LOCUS       DAZFTN010000001       124148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFTN010000001 DAZFTN010000000
VERSION     DAZFTN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 124148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 124148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..124148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..414
                     /locus_tag="ACHKV2_000001"
     CDS             <1..414
                     /locus_tag="ACHKV2_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6937978.1"
                     /translation="GDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIK
                     NSLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPS
                     GHGTVNMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(457..1317)
                     /gene="rsmI"
                     /locus_tag="ACHKV2_000002"
     CDS             complement(457..1317)
                     /gene="rsmI"
                     /locus_tag="ACHKV2_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO6937979.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1381..3417
                     /gene="lpoA"
                     /locus_tag="ACHKV2_000003"
     CDS             1381..3417
                     /gene="lpoA"
                     /locus_tag="ACHKV2_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO6937980.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3375..3770
                     /gene="yraN"
                     /locus_tag="ACHKV2_000004"
     CDS             3375..3770
                     /gene="yraN"
                     /locus_tag="ACHKV2_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO6937981.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            3790..4380
                     /gene="diaA"
                     /locus_tag="ACHKV2_000005"
     CDS             3790..4380
                     /gene="diaA"
                     /locus_tag="ACHKV2_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO6937982.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4390..4965
                     /gene="dolP"
                     /locus_tag="ACHKV2_000006"
     CDS             4390..4965
                     /gene="dolP"
                     /locus_tag="ACHKV2_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6937983.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5079..6119)
                     /gene="yraQ"
                     /locus_tag="ACHKV2_000007"
     CDS             complement(5079..6119)
                     /gene="yraQ"
                     /locus_tag="ACHKV2_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6937984.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6192..6827)
                     /gene="yraR"
                     /locus_tag="ACHKV2_000008"
     CDS             complement(6192..6827)
                     /gene="yraR"
                     /locus_tag="ACHKV2_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6937985.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6955..7473
                     /gene="yhbO"
                     /locus_tag="ACHKV2_000009"
     CDS             6955..7473
                     /gene="yhbO"
                     /locus_tag="ACHKV2_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO6937986.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7453..7896)
                     /gene="yhbP"
                     /locus_tag="ACHKV2_000010"
     CDS             complement(7453..7896)
                     /gene="yhbP"
                     /locus_tag="ACHKV2_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO6937987.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            7947..8249
                     /gene="yhbQ"
                     /locus_tag="ACHKV2_000011"
     CDS             7947..8249
                     /gene="yhbQ"
                     /locus_tag="ACHKV2_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO6937988.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(8236..8739)
                     /gene="yhbS"
                     /locus_tag="ACHKV2_000012"
     CDS             complement(8236..8739)
                     /gene="yhbS"
                     /locus_tag="ACHKV2_000012"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6937989.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8733..9257)
                     /gene="ubiT"
                     /locus_tag="ACHKV2_000013"
     CDS             complement(8733..9257)
                     /gene="ubiT"
                     /locus_tag="ACHKV2_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO6937990.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9466..10461
                     /gene="ubiU"
                     /locus_tag="ACHKV2_000014"
     CDS             9466..10461
                     /gene="ubiU"
                     /locus_tag="ACHKV2_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO6937991.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10470..11348
                     /gene="ubiV"
                     /locus_tag="ACHKV2_000015"
     CDS             10470..11348
                     /gene="ubiV"
                     /locus_tag="ACHKV2_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO6937992.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11429..12436
                     /gene="yhbW"
                     /locus_tag="ACHKV2_000016"
     CDS             11429..12436
                     /gene="yhbW"
                     /locus_tag="ACHKV2_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO6937993.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12553..13797)
                     /gene="mtr"
                     /locus_tag="ACHKV2_000017"
     CDS             complement(12553..13797)
                     /gene="mtr"
                     /locus_tag="ACHKV2_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO6937994.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLALLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13951..15840)
                     /gene="deaD"
                     /locus_tag="ACHKV2_000018"
     CDS             complement(13951..15840)
                     /gene="deaD"
                     /locus_tag="ACHKV2_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO6937995.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15833..15913)
                     /gene="yrbN"
                     /locus_tag="ACHKV2_000019"
     CDS             complement(15833..15913)
                     /gene="yrbN"
                     /locus_tag="ACHKV2_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO6937996.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16020..16904)
                     /gene="nlpI"
                     /locus_tag="ACHKV2_000020"
     CDS             complement(16020..16904)
                     /gene="nlpI"
                     /locus_tag="ACHKV2_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO6937997.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17013..19148)
                     /gene="pnp"
                     /locus_tag="ACHKV2_000021"
     CDS             complement(17013..19148)
                     /gene="pnp"
                     /locus_tag="ACHKV2_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO6937998.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19395..19664)
                     /gene="rpsO"
                     /locus_tag="ACHKV2_000022"
     CDS             complement(19395..19664)
                     /gene="rpsO"
                     /locus_tag="ACHKV2_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO6937999.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(19813..20757)
                     /gene="truB"
                     /locus_tag="ACHKV2_000023"
     CDS             complement(19813..20757)
                     /gene="truB"
                     /locus_tag="ACHKV2_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO6938000.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20757..21158)
                     /gene="rbfA"
                     /locus_tag="ACHKV2_000024"
     CDS             complement(20757..21158)
                     /gene="rbfA"
                     /locus_tag="ACHKV2_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO6938001.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21323..23995)
                     /gene="infB"
                     /locus_tag="ACHKV2_000025"
     CDS             complement(21323..23995)
                     /gene="infB"
                     /locus_tag="ACHKV2_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO6938002.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24020..25507)
                     /gene="nusA"
                     /locus_tag="ACHKV2_000026"
     CDS             complement(24020..25507)
                     /gene="nusA"
                     /locus_tag="ACHKV2_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO6938003.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25535..25987)
                     /gene="rimP"
                     /locus_tag="ACHKV2_000027"
     CDS             complement(25535..25987)
                     /gene="rimP"
                     /locus_tag="ACHKV2_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO6938004.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26194..26270)
                     /locus_tag="ACHKV2_000028"
     tRNA            complement(26194..26270)
                     /locus_tag="ACHKV2_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26234..26236),aa:Met,seq:cat)
     gene            26618..27961
                     /gene="argG"
                     /locus_tag="ACHKV2_000029"
     CDS             26618..27961
                     /gene="argG"
                     /locus_tag="ACHKV2_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO6938005.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(27969..29594)
                     /gene="yhbX"
                     /locus_tag="ACHKV2_000030"
     CDS             complement(27969..29594)
                     /gene="yhbX"
                     /locus_tag="ACHKV2_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6938006.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(30053..30139)
                     /locus_tag="ACHKV2_000031"
     tRNA            complement(30053..30139)
                     /locus_tag="ACHKV2_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30103..30105),aa:Leu,seq:gag)
     gene            complement(30154..30486)
                     /gene="secG"
                     /locus_tag="ACHKV2_000032"
     CDS             complement(30154..30486)
                     /gene="secG"
                     /locus_tag="ACHKV2_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO6938007.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30714..32051)
                     /gene="glmM"
                     /locus_tag="ACHKV2_000033"
     CDS             complement(30714..32051)
                     /gene="glmM"
                     /locus_tag="ACHKV2_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO6938008.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32044..32892)
                     /gene="folP"
                     /locus_tag="ACHKV2_000034"
     CDS             complement(32044..32892)
                     /gene="folP"
                     /locus_tag="ACHKV2_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6938009.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32982..34916)
                     /gene="ftsH"
                     /locus_tag="ACHKV2_000035"
     CDS             complement(32982..34916)
                     /gene="ftsH"
                     /locus_tag="ACHKV2_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO6938010.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35016..35645)
                     /gene="rlmE"
                     /locus_tag="ACHKV2_000036"
     CDS             complement(35016..35645)
                     /gene="rlmE"
                     /locus_tag="ACHKV2_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO6938011.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35771..36064
                     /gene="yhbY"
                     /locus_tag="ACHKV2_000037"
     CDS             35771..36064
                     /gene="yhbY"
                     /locus_tag="ACHKV2_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO6938012.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36220..36696)
                     /gene="greA"
                     /locus_tag="ACHKV2_000038"
     CDS             complement(36220..36696)
                     /gene="greA"
                     /locus_tag="ACHKV2_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO6938013.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36944..38377
                     /gene="dacB"
                     /locus_tag="ACHKV2_000039"
     CDS             36944..38377
                     /gene="dacB"
                     /locus_tag="ACHKV2_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6938014.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38417..39589)
                     /gene="cgtA"
                     /locus_tag="ACHKV2_000040"
     CDS             complement(38417..39589)
                     /gene="cgtA"
                     /locus_tag="ACHKV2_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO6938015.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39605..40570)
                     /gene="yhbE"
                     /locus_tag="ACHKV2_000041"
     CDS             complement(39605..40570)
                     /gene="yhbE"
                     /locus_tag="ACHKV2_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6938016.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40697..40954)
                     /gene="rpmA"
                     /locus_tag="ACHKV2_000042"
     CDS             complement(40697..40954)
                     /gene="rpmA"
                     /locus_tag="ACHKV2_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO6938017.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40975..41286)
                     /gene="rplU"
                     /locus_tag="ACHKV2_000043"
     CDS             complement(40975..41286)
                     /gene="rplU"
                     /locus_tag="ACHKV2_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO6938018.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41545..42516
                     /gene="ispB"
                     /locus_tag="ACHKV2_000044"
     CDS             41545..42516
                     /gene="ispB"
                     /locus_tag="ACHKV2_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO6938019.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42745..43023
                     /gene="sfsB"
                     /locus_tag="ACHKV2_000045"
     CDS             42745..43023
                     /gene="sfsB"
                     /locus_tag="ACHKV2_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO6938020.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43071..44330)
                     /gene="murA"
                     /locus_tag="ACHKV2_000046"
     CDS             complement(43071..44330)
                     /gene="murA"
                     /locus_tag="ACHKV2_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO6938021.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44385..44639)
                     /gene="ibaG"
                     /locus_tag="ACHKV2_000047"
     CDS             complement(44385..44639)
                     /gene="ibaG"
                     /locus_tag="ACHKV2_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO6938022.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44799..45092)
                     /gene="mlaB"
                     /locus_tag="ACHKV2_000048"
     CDS             complement(44799..45092)
                     /gene="mlaB"
                     /locus_tag="ACHKV2_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO6938023.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45092..45727)
                     /gene="mlaC"
                     /locus_tag="ACHKV2_000049"
     CDS             complement(45092..45727)
                     /gene="mlaC"
                     /locus_tag="ACHKV2_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO6938024.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45746..46297)
                     /gene="mlaD"
                     /locus_tag="ACHKV2_000050"
     CDS             complement(45746..46297)
                     /gene="mlaD"
                     /locus_tag="ACHKV2_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO6938025.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46302..47084)
                     /gene="mlaE"
                     /locus_tag="ACHKV2_000051"
     CDS             complement(46302..47084)
                     /gene="mlaE"
                     /locus_tag="ACHKV2_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO6938026.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47092..47901)
                     /gene="mlaF"
                     /locus_tag="ACHKV2_000052"
     CDS             complement(47092..47901)
                     /gene="mlaF"
                     /locus_tag="ACHKV2_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO6938027.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48111..49088
                     /gene="yrbG"
                     /locus_tag="ACHKV2_000053"
     CDS             48111..49088
                     /gene="yrbG"
                     /locus_tag="ACHKV2_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO6938028.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49102..50088
                     /gene="kdsD"
                     /locus_tag="ACHKV2_000054"
     CDS             49102..50088
                     /gene="kdsD"
                     /locus_tag="ACHKV2_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO6938029.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50109..50675
                     /gene="kdsC"
                     /locus_tag="ACHKV2_000055"
     CDS             50109..50675
                     /gene="kdsC"
                     /locus_tag="ACHKV2_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO6938030.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50672..51247
                     /gene="lptC"
                     /locus_tag="ACHKV2_000056"
     CDS             50672..51247
                     /gene="lptC"
                     /locus_tag="ACHKV2_000056"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO6938031.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51216..51773
                     /gene="lptA"
                     /locus_tag="ACHKV2_000057"
     CDS             51216..51773
                     /gene="lptA"
                     /locus_tag="ACHKV2_000057"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO6938032.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51780..52505
                     /gene="lptB"
                     /locus_tag="ACHKV2_000058"
     CDS             51780..52505
                     /gene="lptB"
                     /locus_tag="ACHKV2_000058"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO6938033.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52553..53986
                     /gene="rpoN"
                     /locus_tag="ACHKV2_000059"
     CDS             52553..53986
                     /gene="rpoN"
                     /locus_tag="ACHKV2_000059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO6938034.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54009..54296
                     /gene="hpf"
                     /locus_tag="ACHKV2_000060"
     CDS             54009..54296
                     /gene="hpf"
                     /locus_tag="ACHKV2_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO6938035.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54414..54905
                     /gene="ptsN"
                     /locus_tag="ACHKV2_000061"
     CDS             54414..54905
                     /gene="ptsN"
                     /locus_tag="ACHKV2_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO6938036.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            54951..55805
                     /gene="rapZ"
                     /locus_tag="ACHKV2_000062"
     CDS             54951..55805
                     /gene="rapZ"
                     /locus_tag="ACHKV2_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO6938037.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55802..56074
                     /gene="npr"
                     /locus_tag="ACHKV2_000063"
     CDS             55802..56074
                     /gene="npr"
                     /locus_tag="ACHKV2_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO6938038.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56288..56920
                     /gene="yrbL"
                     /locus_tag="ACHKV2_000064"
     CDS             56288..56920
                     /gene="yrbL"
                     /locus_tag="ACHKV2_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO6938039.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(56917..57645)
                     /gene="mtgA"
                     /locus_tag="ACHKV2_000065"
     CDS             complement(56917..57645)
                     /gene="mtgA"
                     /locus_tag="ACHKV2_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO6938040.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57642..58295)
                     /gene="elbB"
                     /locus_tag="ACHKV2_000066"
     CDS             complement(57642..58295)
                     /gene="elbB"
                     /locus_tag="ACHKV2_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO6938041.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58525..60861)
                     /gene="arcB"
                     /locus_tag="ACHKV2_000067"
     CDS             complement(58525..60861)
                     /gene="arcB"
                     /locus_tag="ACHKV2_000067"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO6938042.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60957..61886)
                     /gene="yhcC"
                     /locus_tag="ACHKV2_000068"
     CDS             complement(60957..61886)
                     /gene="yhcC"
                     /locus_tag="ACHKV2_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO6938043.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62560..67020
                     /gene="gltB"
                     /locus_tag="ACHKV2_000069"
     CDS             62560..67020
                     /gene="gltB"
                     /locus_tag="ACHKV2_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO6938044.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67033..68451
                     /gene="gltD"
                     /locus_tag="ACHKV2_000070"
     CDS             67033..68451
                     /gene="gltD"
                     /locus_tag="ACHKV2_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO6938045.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68635..69684
                     /gene="yhcG"
                     /locus_tag="ACHKV2_000071"
     CDS             68635..69684
                     /gene="yhcG"
                     /locus_tag="ACHKV2_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO6938046.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(69744..70208)
                     /gene="nanQ"
                     /locus_tag="ACHKV2_000072"
     CDS             complement(69744..70208)
                     /gene="nanQ"
                     /locus_tag="ACHKV2_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO6938047.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(70205..71080)
                     /gene="nanK"
                     /locus_tag="ACHKV2_000073"
     CDS             complement(70205..71080)
                     /gene="nanK"
                     /locus_tag="ACHKV2_000073"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6938048.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(71077..71766)
                     /gene="nanE"
                     /locus_tag="ACHKV2_000074"
     CDS             complement(71077..71766)
                     /gene="nanE"
                     /locus_tag="ACHKV2_000074"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6938049.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71814..73304)
                     /gene="nanT"
                     /locus_tag="ACHKV2_000075"
     CDS             complement(71814..73304)
                     /gene="nanT"
                     /locus_tag="ACHKV2_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO6938050.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73413..74306)
                     /gene="nanA"
                     /locus_tag="ACHKV2_000076"
     CDS             complement(73413..74306)
                     /gene="nanA"
                     /locus_tag="ACHKV2_000076"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6938051.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74428..75219)
                     /gene="nanR"
                     /locus_tag="ACHKV2_000077"
     CDS             complement(74428..75219)
                     /gene="nanR"
                     /locus_tag="ACHKV2_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6938052.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75599..76955
                     /gene="dcuC"
                     /locus_tag="ACHKV2_000078"
                     /pseudo
     CDS             75599..76955
                     /gene="dcuC"
                     /locus_tag="ACHKV2_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76998..77495)
                     /gene="sspB"
                     /locus_tag="ACHKV2_000079"
     CDS             complement(76998..77495)
                     /gene="sspB"
                     /locus_tag="ACHKV2_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO6938053.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77501..78139)
                     /gene="sspA"
                     /locus_tag="ACHKV2_000080"
     CDS             complement(77501..78139)
                     /gene="sspA"
                     /locus_tag="ACHKV2_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO6938054.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78534..78926)
                     /gene="rpsI"
                     /locus_tag="ACHKV2_000081"
     CDS             complement(78534..78926)
                     /gene="rpsI"
                     /locus_tag="ACHKV2_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO6938055.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78942..79370)
                     /gene="rplM"
                     /locus_tag="ACHKV2_000082"
     CDS             complement(78942..79370)
                     /gene="rplM"
                     /locus_tag="ACHKV2_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO6938056.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79589..80716)
                     /gene="zapE"
                     /locus_tag="ACHKV2_000083"
     CDS             complement(79589..80716)
                     /gene="zapE"
                     /locus_tag="ACHKV2_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO6938057.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80910..81308
                     /gene="zapG"
                     /locus_tag="ACHKV2_000084"
     CDS             80910..81308
                     /gene="zapG"
                     /locus_tag="ACHKV2_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO6938058.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81462..82829
                     /gene="degQ"
                     /locus_tag="ACHKV2_000085"
     CDS             81462..82829
                     /gene="degQ"
                     /locus_tag="ACHKV2_000085"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO6938059.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82919..83986
                     /gene="degS"
                     /locus_tag="ACHKV2_000086"
     CDS             82919..83986
                     /gene="degS"
                     /locus_tag="ACHKV2_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO6938060.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(84050..84988)
                     /gene="mdh"
                     /locus_tag="ACHKV2_000087"
     CDS             complement(84050..84988)
                     /gene="mdh"
                     /locus_tag="ACHKV2_000087"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6938061.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85423..85893
                     /gene="argR"
                     /locus_tag="ACHKV2_000088"
     CDS             85423..85893
                     /gene="argR"
                     /locus_tag="ACHKV2_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO6938062.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86258..86521
                     /gene="yhcN"
                     /locus_tag="ACHKV2_000089"
     CDS             86258..86521
                     /gene="yhcN"
                     /locus_tag="ACHKV2_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO6938063.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86577..86849)
                     /gene="yhcO"
                     /locus_tag="ACHKV2_000090"
     CDS             complement(86577..86849)
                     /gene="yhcO"
                     /locus_tag="ACHKV2_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO6938064.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86941..88908)
                     /gene="aaeB"
                     /locus_tag="ACHKV2_000091"
     CDS             complement(86941..88908)
                     /gene="aaeB"
                     /locus_tag="ACHKV2_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO6938065.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88914..89843)
                     /gene="aaeA"
                     /locus_tag="ACHKV2_000092"
     CDS             complement(88914..89843)
                     /gene="aaeA"
                     /locus_tag="ACHKV2_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO6938066.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(89851..90054)
                     /gene="aaeX"
                     /locus_tag="ACHKV2_000093"
     CDS             complement(89851..90054)
                     /gene="aaeX"
                     /locus_tag="ACHKV2_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO6938067.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            90237..91166
                     /gene="aaeR"
                     /locus_tag="ACHKV2_000094"
     CDS             90237..91166
                     /gene="aaeR"
                     /locus_tag="ACHKV2_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO6938068.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(91300..92745)
                     /gene="tldD"
                     /locus_tag="ACHKV2_000095"
     CDS             complement(91300..92745)
                     /gene="tldD"
                     /locus_tag="ACHKV2_000095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO6938069.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(92901..96701)
                     /gene="yhdP"
                     /locus_tag="ACHKV2_000096"
     CDS             complement(92901..96701)
                     /gene="yhdP"
                     /locus_tag="ACHKV2_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO6938070.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(96769..98238)
                     /gene="rng"
                     /locus_tag="ACHKV2_000097"
     CDS             complement(96769..98238)
                     /gene="rng"
                     /locus_tag="ACHKV2_000097"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO6938071.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(98228..98821)
                     /gene="yhdE"
                     /locus_tag="ACHKV2_000098"
     CDS             complement(98228..98821)
                     /gene="yhdE"
                     /locus_tag="ACHKV2_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO6938072.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(98830..99318)
                     /gene="mreD"
                     /locus_tag="ACHKV2_000099"
     CDS             complement(98830..99318)
                     /gene="mreD"
                     /locus_tag="ACHKV2_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO6938073.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(99318..100421)
                     /gene="mreC"
                     /locus_tag="ACHKV2_000100"
     CDS             complement(99318..100421)
                     /gene="mreC"
                     /locus_tag="ACHKV2_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO6938074.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            complement(100487..101530)
                     /gene="mreB"
                     /locus_tag="ACHKV2_000101"
     CDS             complement(100487..101530)
                     /gene="mreB"
                     /locus_tag="ACHKV2_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO6938075.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(101835..103775)
                     /gene="csrD"
                     /locus_tag="ACHKV2_000102"
     CDS             complement(101835..103775)
                     /gene="csrD"
                     /locus_tag="ACHKV2_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO6938076.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            103927..104901
                     /gene="yhdH"
                     /locus_tag="ACHKV2_000103"
     CDS             103927..104901
                     /gene="yhdH"
                     /locus_tag="ACHKV2_000103"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO6938077.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            105879..106349
                     /gene="accB"
                     /locus_tag="ACHKV2_000104"
     CDS             105879..106349
                     /gene="accB"
                     /locus_tag="ACHKV2_000104"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO6938078.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            106360..107709
                     /gene="accC"
                     /locus_tag="ACHKV2_000105"
     CDS             106360..107709
                     /gene="accC"
                     /locus_tag="ACHKV2_000105"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO6938079.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            107818..108060
                     /gene="yhdT"
                     /locus_tag="ACHKV2_000106"
     CDS             107818..108060
                     /gene="yhdT"
                     /locus_tag="ACHKV2_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO6938080.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            108050..109501
                     /gene="panF"
                     /locus_tag="ACHKV2_000107"
     CDS             108050..109501
                     /gene="panF"
                     /locus_tag="ACHKV2_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO6938081.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            109513..110394
                     /gene="prmA"
                     /locus_tag="ACHKV2_000108"
     CDS             109513..110394
                     /gene="prmA"
                     /locus_tag="ACHKV2_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO6938082.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            110723..111688
                     /gene="dusB"
                     /locus_tag="ACHKV2_000109"
     CDS             110723..111688
                     /gene="dusB"
                     /locus_tag="ACHKV2_000109"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO6938083.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            111714..112010
                     /gene="fis"
                     /locus_tag="ACHKV2_000110"
     CDS             111714..112010
                     /gene="fis"
                     /locus_tag="ACHKV2_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO6938084.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            112095..112979
                     /gene="yhdJ"
                     /locus_tag="ACHKV2_000111"
     CDS             112095..112979
                     /gene="yhdJ"
                     /locus_tag="ACHKV2_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6938085.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            113063..113242
                     /gene="yhdU"
                     /locus_tag="ACHKV2_000112"
     CDS             113063..113242
                     /gene="yhdU"
                     /locus_tag="ACHKV2_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO6938086.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(113245..113907)
                     /gene="acrS"
                     /locus_tag="ACHKV2_000113"
     CDS             complement(113245..113907)
                     /gene="acrS"
                     /locus_tag="ACHKV2_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO6938087.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            114306..115463
                     /gene="acrE"
                     /locus_tag="ACHKV2_000114"
     CDS             114306..115463
                     /gene="acrE"
                     /locus_tag="ACHKV2_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO6938088.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            115475..118624
                     /gene="acrF"
                     /locus_tag="ACHKV2_000115"
                     /pseudo
     CDS             115475..118624
                     /gene="acrF"
                     /locus_tag="ACHKV2_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            118877..119098
                     /locus_tag="ACHKV2_000116"
     CDS             118877..119098
                     /locus_tag="ACHKV2_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938089.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            119529..120554
                     /locus_tag="ACHKV2_000117"
     CDS             119529..120554
                     /locus_tag="ACHKV2_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6938090.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            120622..121803
                     /gene="yhdX"
                     /locus_tag="ACHKV2_000118"
     CDS             120622..121803
                     /gene="yhdX"
                     /locus_tag="ACHKV2_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6938091.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            121813..122916
                     /gene="yhdY"
                     /locus_tag="ACHKV2_000119"
     CDS             121813..122916
                     /gene="yhdY"
                     /locus_tag="ACHKV2_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6938092.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            122924..123682
                     /gene="yhdZ"
                     /locus_tag="ACHKV2_000120"
     CDS             122924..123682
                     /gene="yhdZ"
                     /locus_tag="ACHKV2_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO6938093.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(123913..124028)
                     /gene="rrf"
                     /locus_tag="ACHKV2_000121"
     rRNA            complement(123913..124028)
                     /gene="rrf"
                     /locus_tag="ACHKV2_000121"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(124068..124143)
                     /locus_tag="ACHKV2_000122"
     tRNA            complement(124068..124143)
                     /locus_tag="ACHKV2_000122"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124108..124110),aa:Thr,seq:ggt)
BASE COUNT        30276 a        33106 c        31011 g        29755 t
ORIGIN      
        1 ggcgatgtta ttaaggttaa ttttggattc atcaatggtc agaagtttac cacccgcaat
       61 gccatgccag acggttttac tccagtagac tttgatatca cttatgactg tggtgatact
      121 tcaaagatta aaaactcgtt gcaaatgcgc atcgacggta caactggggt agtagaccag
      181 tacaacctgg tcgccagacg aagaagttca gacaatgcgc ccgatgtcgg tattcgtatt
      241 gaaaatctcg gcggcggagt tgcaaatatt ccttttcaga acggtatcct tcccgttgat
      301 ccttccgggc atggcaccgt caatatgcgc gcctggccag ttaatctggt cggtggtgag
      361 ctggaaacag gaaaatttca gggcacagcc accattaccg tcatcgtgcg gtaaaagaag
      421 acgttgcgtt ttgattaacg caacgcttcg gcactcttac ccctgctgct ccagcgcata
      481 cttatacagc gcatttttct tcacgccgtg aatttctgcg gccagcgccg ccgctttttt
      541 cagcggcagt tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc
      601 ttcctgtgct ttatgacctt cgacaatcag caccatttcg cctttgcgac ggttttcatc
      661 ttcctttacc cacgccagca gctcgccaac gggcgcgccg tgaatggttt cccaggtttt
      721 ggtcagctca cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc
      781 caggctatct aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc
      841 aatggctttt agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca
      901 gaaacggtca gagggtaaac ccgcggcgct taacgcagtg atagcagcac acggtccggg
      961 tagcggcacc acacgaatcc ccgcttcacg gcaggtacgc accagatggt agccaggatc
     1021 gttaattagc ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagctt
     1081 cgccagcagc gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc
     1141 attaatccca aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag
     1201 atcaacggcc tgtaatacct ctaacgcacg ctgggtgata tccgccagat tgccgattgg
     1261 cgtcggtaca atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt
     1321 gtcgtccgta ttgccgattt aatattgagc attgcgtaaa aaaatatcac tggatacatt
     1381 atggtaccct caacattttc tcgtttgaaa gccgcgcgtt gtctgcctgt tgttctggca
     1441 gccctgattt tcgtcggttg tggcacccat actcccgatc agtccactgc ttatatgcag
     1501 ggcacggcgc aggctgattc tgccttttat cttcagcaga tgcagcaaag ctctgatgat
     1561 accaggatca actggcaatt actcgccatt cgtgcactgg tgaaagaagg taaaaccggg
     1621 caggcggttg agttgtttaa ccaactaccg caagaactga acgattctca gcgtcgcgag
     1681 aaaacactgc tggcggtaga gattaaactg gcgcagaaag attttgctgg cgcgcaaaac
     1741 ttgctggcga aaattacacc cgccgattta gaacaaaacc agcaagcccg ttactggcag
     1801 gcaaaaatcg atgccagcca ggggcgtcct tccattgatt tactgcgcgc gttaattgct
     1861 caggaaccgc tgcttggcgc gaaagaaaaa cagcagaata ttgatgccac ctggcaggcg
     1921 ctctcctcca tgactcagga acaggcgaat acgctggtga tcaacgccga cgaaaatatt
     1981 ctgcaaggct ggctggatct gcagcgcgtc tggtttgata accgtaacga tcccgacatg
     2041 atgaaagccg ggatcgccga ctggcagaaa cgttatccga acaatccggg cgcgaaaatg
     2101 ctgccaacgc agttggttaa cgtaaaagcg tttaaaccag cctcgaccaa caaaatcgcc
     2161 ctgctgttgc cactgaatgg ccaggcagcg gtatttggtc gcactattca gcaaggcttt
     2221 gaagcggcga aaaatatcgg cactcagcca gtggcggctc aggtagctgc cgcacctgcc
     2281 gcagacgtag cagaacaacc tcagccgcaa actgcggatg gcgttgccag cccggcacaa
     2341 gcctcggtta gcgatctgac cggtgatcag cctgcggccc agccggtgcc tgtaagcgcc
     2401 ccggcgacaa gcaccgcagc ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc
     2461 tacgatacct catcacaacc acttagccag atcttaagcc aggttcagca ggatggcgcg
     2521 agtattgtgg ttggtccgtt gctgaaaaat aacgttgaag agttgctgaa gagcaacact
     2581 ccgctgaacg tactagcact gaaccagccg gagaatatcg aaaatcgcgt caatatttgt
     2641 tacttcgcgc tttcaccgga agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag
     2701 ggtaaacaag cgccgctggt gctgatccca cgcagttcat tgggcgatcg cgtagccaat
     2761 gcgtttgcgc aagagtggca gaaattgggc ggcggcaccg ttctgcaaca aaaatttggt
     2821 tccaccagcg aattacgcgc gggtgttaac ggcggttctg gtattgcttt aacgggtacc
     2881 ccgattactc ccagagcgac aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa
     2941 accacgccaa ccgatgacca gttcaccaat aatggcggtc gtgtcgatgc ggtgtacatt
     3001 gtggcaacgc cgggtgaaat cgcttttatt aaaccgatga tcgccatgcg taacggtagc
     3061 cagagcggtg caacgctgta cgccagctcc cgcagtgcgc aagggaccgc tggcccggat
     3121 ttccgtctgg agatggaagg cttgcagtac agcgaaatcc cgatgctggc gggcggtaat
     3181 ctgccgttaa tgcagcaggc actcagcgcg gtgaataacg attattcact ggctcgcatg
     3241 tatgcgatgg gcgtcgatgc ctggtcgctg gcaaatcatt tctcacaaat gcgccaggtt
     3301 cagggttttg aaatcaacgg taataccgga agcctgacgg ctaacccgga ttgcgtgatt
     3361 aacaggaagt tatcatggct acagtaccaa caaggtcagg tagtccccgc cagttaacca
     3421 ccaaacagac cggcgatgcg tgggaagtac aagcgcgtcg ctggctggaa ggcaaaggac
     3481 tgcgatttgt cgccgctaac gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg
     3541 aaggccggac caccgttttt gtcgaggtgc gttaccgccg ttctgcgctt tatggcggcg
     3601 cggcagccag tgtgacccgc agcaaacaac acaaattatt acagactgcc cgcttgtggc
     3661 tcgcgcgtca taatgggagt tttgatactg tggattgccg gttcgatgtg gtagccttca
     3721 ccgggaatga ggttgagtgg attaaggata cctttaatga ccactcataa ttaaggttta
     3781 aggattagcg tgcaagaaag aattaaagct tgcttcactg aaagcattca aactcaaatt
     3841 gcggcggcag aggcgcttcc ggatgccatc tcccgtgcgg ccatgacgct ggttcagtct
     3901 ctgctcaatg gcaacaaaat cctctgttgt ggtaatggaa cttccgctgc caatgcacag
     3961 cattttgctg ccagcatgat caaccgtttc gaaacggagc ggcccagctt acctgccatt
     4021 gcactaaata ccgataatgt tgtcttaacg gcgattgcca acgatcgctt acatgatgaa
     4081 gtgtatgcaa aacaggtgcg ggcgctgggg catgcgggag atgtattgtt agccatttcc
     4141 acccgtggca acagccgcga tatcgtaaaa gcagtagaag ctgccgttac gcgtgatatg
     4201 accattgtgg cattgaccgg ttatgacggc ggtgaacttg caggtttgtt agggccacag
     4261 gatgtggaga tccgcatccc ttcgcatcgc agtgctcgca ttcaggaaat gcatatgctg
     4321 acggtaaatt gcctgtgcga tctgatcgat aacacgcttt tccctcacca ggatgattaa
     4381 ggagaataca tgaaggcatt atcgccaatc gcagtcctta tttccgcgct gctgttgcaa
     4441 ggttgtgttg ccgctgccgt agtgggtact gctgctgtgg gtaccaaagc cgcaactgac
     4501 ccacgcagtg tcggcaccca ggtggacgat ggtaccctgg aagtgcgcgt gaacagcgca
     4561 ttgtcgaaag acgaacagat taagaaagaa gcgcgcatta atgtaacggc ctatcagggc
     4621 aaagtgctgc tggttgggca gtcaccaaac gctgaacttt cggctcgcgc caaacagatt
     4681 gctatgggcg tagacggtgc caacgaagtg tataacgaga ttcgtcaggg ccagccgatt
     4741 ggtctgggcg aagcgtctaa cgatacgtgg atcaccacca aagtgcgttc gcagctctta
     4801 accagcgacc tggtgaaatc gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc
     4861 ctgatggggc tggtgactga acgtgaagcg aaagcggcgg cagatattgc cagccgggtg
     4921 agcggcgtga agcgggtaac tacggcgttt acgtttatta aatagcagcc ccttgtaatg
     4981 cctgatgcga cgcttgccgc gtcttatcag gcctacaacg atgcaaaccg taggtcggat
     5041 aaggcgttta cgccgcatcc gacatgtttt ccctcaaatc agaataacag cgccagcccg
     5101 ccgacaatca cgcctgacac tgccaccatc gcccccgtca gccataaggc tttcgccggg
     5161 aacgctttgc gcagcataat cagtgacggc aaactcaccg ccgggagcgt catcaacaat
     5221 gccagcgccg gagcagttcc catacctgcc agcatcatcg tttgtacaat cggaatttct
     5281 gctgccgtgg gaatgacaaa caagcaccct gctaccgcca tcgccaccac ccacatcagg
     5341 ctgttatcga cagcaccatc ggcatggggg aataaccaga cgcgagcggc acccaacacc
     5401 agtactgcaa ggatgtaaac cgggatcgta ctccagaaaa gcgtccatag cgccctgccc
     5461 cagcgactaa aaaatccgcc ctgtgcttcc ggtatgtcaa tttcgaccgg tgcttgcgtt
     5521 tgcggtgttt cacgcaccca tttttgcacc agcgtcgcaa tcagcaacac catcaccagc
     5581 ccggccacca gacgaatcgc cgcaaaaccc cagccgagga caaaccccat aaacaccagc
     5641 gtcgccgggt ttaacaccgg attgcccatc cagaatgcca gcgcaccgcc catcgacacc
     5701 tgttggcgac gcattcccgc cgcgaccgga gccgcacagc aggtacacat catgcctggc
     5761 agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac
     5821 aaccagtcac gcgggatcag cacctgaatc aacgaaccga ggatcacccc cagcaccgcc
     5881 gctttccata ccgcgaggaa atagatcatc gcgtaatcca acgccgcctg ccatggatta
     5941 gcatccgcct gcgcaaggat agatttaccg atactgtggg tttcggcagc agtaaacgct
     6001 ttgccgtagt aaggttgcca tttcacatac cagagaccgg caatgacaac gagaaagaaa
     6061 agcgcgggtt tccaccactg aatgggcgtt gccgcctgag atgaagactg accagtcata
     6121 gcattcccca ggaataagtt atgataattg agcgcgtgaa tattacgctc actatcaatt
     6181 cttgggaata attattcagc tcttttgcgt aattctgaag agcttaaaat cgtcacacct
     6241 tcatgctcgg gccgcatcgc ctctgccagc atgacccgcg ccacgtctct cgcatcaatg
     6301 gatttccagt taccgggtaa caaacggaac agcggcgcaa aaagcgtttc gttcatccgc
     6361 tgtttgctac gatcgcccag taacatcgac gggcgagcaa tggtcaattt cggccagttc
     6421 tgggcgatta atgcttcttc catctcccct ttgacgcggt tatagaaaaa cggcgagtgg
     6481 gcattggcac ccatagcact gaccaccagc atatgctgcg cgcccagtcg ccgcccggtt
     6541 aatgcggtat ccactaccag cgtgtaatcg gcatgaataa acgcctcttt gctccccgct
     6601 tctcgccgcg tggtgccgag acaacaaaac acaatgtcga tgggatcggt gacctgcgcc
     6661 agcgcgtcag tcagttgcgg atcatgggga ttaaataccc cgggcatatc gcccaacgga
     6721 cgtcgcgtcg gcgcggctat ggcgttaact ttcggttcgt taatcaacat ccgcagcagg
     6781 tgaccgccca ccagccccgt tgcgcctgta atcagtacct gactcatctt cgctccttta
     6841 cagaattgtc tgccttgcgc tccacggctc atgcaccagg cttaatagac catgaggtaa
     6901 ttatccccga ttgtgggaaa ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt
     6961 aagaaaattg ccgttttaat cactgatgaa tttgaggatt cagaatttac ttcacccgca
     7021 gacgagttcc gtaaagccgg acacgaagtg attaccattg aaaaacaagc gggtaaaacg
     7081 gtgaaaggca aaaaaggaga agccagcgtg accatcgata aatccatcga tgaagtgacg
     7141 ccagcggagt ttgatgccct gttgctaccg ggcggtcatt caccggatta tctgcgtggc
     7201 gacaaccgtt ttgtcacctt tacccgtgat tttgtgaata gcggcaaacc tgttttcgcc
     7261 atctgtcacg gtccgcagct gctgatcagc gccgatgtga ttcgggggcg caaactgacc
     7321 gcggttaaac cgatcattat tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa
     7381 gtcgtggtcg ataaagatca gctggttacc agccggacac cggacgatct gccagcgttt
     7441 aaccgcgagg cgttacgcct gctcggtgcc tgagccgcgt agccaaatca tttttttacc
     7501 aaagcccagc gtgttgtcgg tgaatttgat ttcatcgggc cggatttccc acaccggggc
     7561 cgacagcatt ctggcaaccg gaaagcgacg attgtacgcc ttgcgcgcga ggtcgctttc
     7621 ctcaccttcc agcctgcgga tctcaccttt aaactgcacg ccgcgaatta acgctaccgt
     7681 tttcggctga ccgtttaccg ttccggcaac cgcagcctgc ggcccactca tctgcgcgtg
     7741 gcgcgttttt tcttccgtca aaatgtagaa ggcgactttc tgcacatcaa acagataaaa
     7801 ggcattggcg caccagagtt ccccttcctg ctgcacacac caggtgacaa catgttgttt
     7861 tgccagccaa cggctgatgg cggtgagtgt ttccattgct gttctctttt atactgtggg
     7921 cctgacctta acatgcgacg cagacgatga caccctggtt tctttacttg atccgtaccg
     7981 ccgacaataa gctttatacc gggatcacca cggatgtcga acgccgctat cagcagcacc
     8041 aaagcggcaa aggggcgaaa gcactgcgcg ggaaaggaga actgacgctg gcgttttctg
     8101 cgccagtggg cgatcgttcg ctggcgttac gggcggaata tcgcgttaag caactgacca
     8161 aacggcaaaa agagcgtctg gtagcggaag gcgcagggtt tgcaaagctg ttaagcagtc
     8221 tgcaaacccc ggagattaaa agcgattgaa atgctcgtga tactcaacca gaccagttac
     8281 gccattcaac gcgtcatccg ctaaacgatg tacctggaag gcgctttcag tgcccggcca
     8341 gcggcaacgc agatcgtgat gcgccgccag ttcaaagccg aaacggctgt atagcgccgg
     8401 atcgcccagc gtcaccactg cggcatagcc gaactcatta agcgaatcga gtccttcata
     8461 gaccagttgg cgtgccagcc cctgtccacg gtatttttca tctaccgcca gcggtgccat
     8521 gccgacccat tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca catagccaat
     8581 gacctgacct tcgtcatctg tcgccaccag ccccagcgtc agaaagccat cttcacgcag
     8641 atcgtgaacc agcttcgctt ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat
     8701 acccggcgca tcaatgggaa tttctactcg aattagcatg gttcacctac cgatgtctgt
     8761 ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg cagcagcatc
     8821 atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt tttcacatac
     8881 agccccagct ccgtatcgcc ttcaatcacc agccgacgct ggaagaagag cgtatccgga
     8941 tcttgtttac gcgccgcaat catcagcaga tcgctggcgt cggcactaaa actcacatca
     9001 gcttgcgcgt tctggctgac caccagtttg ccattcacca ccgaggtaaa ccattgcagg
     9061 tcaatatcac gcacatgaat acttaaccag cggccttcaa gaaactccag ctcaccatca
     9121 tccagcgcct ggcggaattg ccagcttaag acctgctcaa gaacctggcg ttttagcgca
     9181 aatggcgtca gttttaccgg tacactcaac agagatggcc ccaaatgcac aatacgggaa
     9241 cgcagtttat ccaacacgag ttttactccc tgtttcaaca atcatcctat tttgccatat
     9301 cagaaaaata acatagcggt ataaatcaac aattccatat gaaattgctg ctaccaccaa
     9361 tacaacttta actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt
     9421 cgttaacatt tttgcgtttt gatagcgcaa ccttcaggaa aaattatgga gctgctctgc
     9481 cctgccggaa atctcccggc gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat
     9541 atcgggctaa aagatgatac caatgcccgt cacttcgccg gccttaactt taccgagaaa
     9601 aaattgcagg aagcggtgag ttttgtccat caacatcgcc gcaaacttca catcgcgatt
     9661 aacacttttg cgcatccgga cggttacgcc cgttggcagc gtgccgtgga tatggcggcg
     9721 cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca tgctagagta cgccgccgag
     9781 cgttacccgc atattgagcg tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg
     9841 attaactttt atcatcgcca ttttgacgtt gctcgcgtgg tgctgccgcg cgtgttgtcg
     9901 attcatcagg tgaaacaact ggcacgggtc acacctgtcc cgctggaagt ctttgctttc
     9961 ggcagcctgt gcattatgtc ggaaggtcgt tgctatctgt cgtcgtatct gacgggtgag
    10021 tcgcccaaca ccgtgggcgc gtgttctccg gcccgtttcg tgcgctggca gcaaacgccg
    10081 caggggctgg aatcccgcct gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac
    10141 gcaggttatc cgacgctatg taaagggcgt tatctggtgg acggcgagcg ctatcacgcg
    10201 ctggaagaac caaccagtct caataccctg gaactgctgc cggagttaat ggcggcgaat
    10261 attgcttcgg tgaaaattga aggccgccaa cgtagcccgg cgtatgtcag ccaggtggcg
    10321 aaagtctggc gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa
    10381 agcgcgtgga tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg
    10441 tatcaccgta aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt
    10501 actggccaaa agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg
    10561 tgatttatct tggcgaagcg gtttgcagca agcgccgggc aaccaaagtt ggcgactggc
    10621 tggagatggc aaaatcgctc gccggaagtg gtaagcagat tgtgctctcc acgctggcgc
    10681 tggtgcaggc atcatctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc
    10741 tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg
    10801 tcgccggaca cgcgctgaac tgctataacg cggtgacgct gaaaatattg ctcaaacagg
    10861 gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta
    10921 atcagtgcga tgagctgggc attcgcaacc agtttgaagt tgaagtcctg agctacggtc
    10981 atctaccgct ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag
    11041 atgagtgtga aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa
    11101 accaacaagt gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg
    11161 gtaacgagct ggcatccatg cagggcctgg ttgatgtggt ccgcctgtca ccgcagggta
    11221 ctgacacttt cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcaccactgc
    11281 cgttgacggc gaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc
    11341 aagcctaagt aaatagctca ctttgttaac aactttaact actctttaat gcagtattaa
    11401 agattaatcg gtaacaaagt gagctgttat gactgataaa accattgcgt tttcgctact
    11461 cgatctggcc cccattcccg aaggttcttc agcgcgagat gcattctccc actctctcga
    11521 tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag aacaccacaa
    11581 tatgactggc attgccagtg ctgccacgtc ggtattgatt ggctatctgg cggcgaatac
    11641 caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac cgttggtcat
    11701 tgcagaacag ttcggcacgc tcaatacact ctatccgggg cgaatcgatt tggggctggg
    11761 tcgtgctccg ggtagtgacc agcgaaccat gatggcgcta cgtcgtcata tgagcgggga
    11821 tattgataat ttcccccgcg atgtcgcgga gctggtggcc tggtttgacg cccgcgatcc
    11881 caatccgcat gtgcgcccgg taccaggcta cggcgagcaa atccccgtgt ggttgttagg
    11941 ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgtttg cgtttgcctc
    12001 acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagca acttcaaacc
    12061 gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ctgccgacag
    12121 caaccgcgac gctgaatttc tgtttacctc aatgcagcaa gcctttgtga agctgcgccg
    12181 tggcgaaacc gggcaactgc cgccgccgat tcaaaatatg gatcagttct ggtcaccgtc
    12241 tgagcagtat ggcgtgcaac aggcgctgag tatgtcgctg gtgggcgata aagcgaaagt
    12301 gcgtcatggt ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggttaacgg
    12361 gcagattttc gaccaccagg cgcgcctgca ttcgtttgag ctggcgatgg atgttaagga
    12421 agagttgttg ggatagtgtg tcttaacgcg ggaagcctta tccgagctgg caacgctgtc
    12481 ccacgtaggc ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat
    12541 aaggcccgct gattactgat acaccggcag caaattaaag ctcgataaga tatggaccag
    12601 tgcgttgcca acgccaaata ccagaatcag cgtaatcatc ggcttgccgc cccagacgcg
    12661 gaatttcggg ctgccaaagc gtttacgcga tgcgcgggcc agcagtgccg gaacaattgc
    12721 cgcccagatg gtagctgcta aaccagcata accaatggcg tacaggaatc cgttcgggaa
    12781 cagcagacca cccacaacag gaggggcaaa ggtcagcaat gccgttttca agcggcccac
    12841 agccgagtcg tcgaaaccaa acagatctgc cagatagtca aacaaaccca gcgttacgcc
    12901 gaggaacgaa ctcgctaccg caaagtttga gaacacgacc agcagcagag ccagactacg
    12961 gctgttcagt acgccgctta acgcctgtac cagcacatca atattaccgc ccttctctgc
    13021 aatatcgata aactccggac gcgggatgtt acccatcgtc gccagcaacc agatggtata
    13081 cagcgccagc gccatcagcg taccgtacac cagacatttc acgatggttt tcggatcttt
    13141 gccgtaatac ttcatcaggc ttggcacgtt accgtgataa ccaaacgatg ccagacagaa
    13201 cggcaaggtc atcaacagat acggtgcata agacgcattg ctttcagcga cgttgaacaa
    13261 tgtcgcaggc tgcacatgcc ccagcaggct accgaaggtg aggaagaagg taatgacttt
    13321 cgcccccaat acaatcgctg tcatgcgact gacggcttta gtgctcaacc acaccacaaa
    13381 cgctaccagc aatgcaaaac caaaacccgc cgcccgtgcc gggacgttta gtgacatctc
    13441 tgcgaaggtg tgatgcagaa tcgaaccact ggcagaaata taggcatagg tcaggatata
    13501 gagcacaaag gcaatggaaa tgccgttgac cacgttccag cctttgccca gcaaatcttt
    13561 ggtgatggtg tcaaaactcg aaccgattct gtaattcagg ttagcttcca gaatcatcaa
    13621 gccggaatgc agcatacaga accaggtaaa gatcagcgcc gccattgacc agaaaaacca
    13681 cgccccggac atgaccactg gcagagaaaa catccctgcg ccaataatgg tgccgccgat
    13741 aatcaccacg ccgccaagca gcgacggtga cgtttgggtg gtggttagtg ttgccatgag
    13801 ggcttctctc cagtgaaaaa tagtgcgact gcgttgttat gtattgcact gtaccagtac
    13861 gcgagtacaa aagacagaaa aaaagccccg atggttcaaa tcggggctgt atatattatt
    13921 ttacagactg tgttcgctgt tcagcgatga ttacgcatca ccaccgaaac gacgacgacc
    13981 ggtagagtca tcacgacgcg gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc
    14041 atcaccacga ccaccttcac ggcgttcacc gctgaagtta cgaccacctt cacgacgctc
    14101 gccactgaaa ccacgaccac cgccacgacg ctctccgcca gtatgcggct gtgcatcgcc
    14161 cagtaattgc atattcatcg gcttgttcag aatgcgagta cgtgtaaagt gttgcagcac
    14221 ttcacccggc atacctttcg gcagttcgat ggtggagtga gaagcaaaca gcttaatgtt
    14281 accgatgtaa cggctgctga tgtcgccttc gttagcaatc gcaccaacga tatgacgaac
    14341 ttcaacacca tcatcgcggc ccacttcaat acggtacaac tgcatgtcac caacatcacg
    14401 acgttcacga cgcggacgat cttcacggtc gccacgcgga ccacggtcgt tacgatcgcg
    14461 cggaccacgg tcatcacggt cacggaattc acgtttcgga cgcatcggcg catctggcgg
    14521 aacaatcaga gtacgttcac cctgtgccat tttcagcagt gcggcggcca gagtttcgag
    14581 atccagctct tcaccttctg cagtcggctg aattttgctc agcagtgcgc ggtattgatc
    14641 cagatcgctg ctttccagct gctgctgtac tttagcggcg aatttttcca gacggcgttt
    14701 gcccagcagt tctgcgttcg gcagttctac ttccggaata gtcagcttca tagtacgttc
    14761 aatgttgcgc agcagacgac gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg
    14821 acccgcacga ccggtacgac cgatacggtg aacgtaagac tcagaatcca tcgggatatc
    14881 gtagttaacc accaggctga tacgctcaac gtccaggcca cgggctgcaa cgtcggtcgc
    14941 aatcaggatg tccagacgac catctttcag gcgttccagt gtctgctcac gcagcgcctg
    15001 gttcatgtca ccgttcagcg cggcgctgtt gtagccgtta cgctcaagag cttcagccac
    15061 ttccagagtc gcatttttgg tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc
    15121 caggaaacgt accagtgctt cgtttttacg cataccccag acagtccaat agctctggct
    15181 gatgtcagga cgggtagtca cgctggactg aatgcgcact tcctgcggct ctttcataaa
    15241 gcggcgggta atgcgacgaa tcgcttccgg catggttgca gagaacagag cggtctgatg
    15301 accttccggg atctgcgcca taatagtttc aacgtcttcg atgaagccca tgcgcagcat
    15361 ttcgtcagct tcatccagaa ccagaccgct cagtttagag aggtccagag tgccacgttt
    15421 caggtggtcc agcagacggc ccggagtacc gacaacgatc tgcggcccct gacgcagggc
    15481 gcgtaattgc acgtcataac gctggccgcc gtacagggca accacattta cgccgcgcat
    15541 gtgtttagag aaatccgtca ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc
    15601 tagcaccaga atctgtggtg ctttcagctc aggatcaaga ttttgcagca gaggtaaaga
    15661 gaatgctgca gtttttccgc tccccgtctg ggccataccc agtacgtcgc ggccattcag
    15721 cagatgtgga atacactccg cctgaattgg agatggtttt tcgtaaccca gatcgttaag
    15781 ggcttcaagg ataggagcct tcaggcccag atctgcaaaa gtggtttcga attcagccat
    15841 gtagtacgtg tgcctcaaaa ttaatggcgg ccagtctaca taactcatca tgaaattgat
    15901 cagcaatttt cattgaaaag tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac
    15961 gccctcaccc gttaaggtga tggcaatcaa aaaagattac gggctgatgt gtacgtcagc
    16021 tattgctggt ccgattctgc caggtcatct tggtcctggc ccaggagcga taattccaac
    16081 aatgcgtatc ggtgctcaac aaagttatga acgttgttgg caaccgccag tttgaacagt
    16141 gccgtggcgc tgtccaaatc ccccagactt aggtagtact tacctaaata gaagttggtt
    16201 tcactgagat gctcagcgag cgaggtgtta tccgttgcgt ccgccttgag cctttccatt
    16261 aacgtttgtt cgctaatgtt gcccaggtag aactcgacaa tgttccatcc ccactgttcc
    16321 ttatccgatt tttcgaagtg ctgtttcaac acttctttag cctgcttctc atcgagcttc
    16381 tgctcggcga gataaagcca cagactacgg aaaggatcat tgggatcgtc ttgataaaac
    16441 gccagcagat catcttgcgc taacttgtca cgaccgccgt aatataatgc gatcccgcga
    16501 ttcaagtgcg cgtagttgta agttggatca agctcaagta cagaatcaaa cgcttcatag
    16561 gcagcatcaa aattgcctgc ctgcgttaaa tatatgccta agtaattgaa tacttcaggc
    16621 atatccggtc ggattgccag cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg
    16681 agactatcat acaacactcc gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa
    16741 gcccgactgg caaggatttg ttccatacgt gccagaatca cttcctgctg taaagtcggt
    16801 tgcaatggta ccgcgaggac ttcactttta cgccaggaag tattactgca tcctgcaagc
    16861 gtaagtgctg tcgcaacgaa acaccagcgc aaaaaaggct tcatttccca ctcccgaaga
    16921 ccacggttga atgaacgtcc tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag
    16981 ccccccgccg cagcggaggg caaatggcaa ccttactcgc cctgttcagc agccggagct
    17041 tccggtgctg cagcaggttg agactgctca gtcgcttctt taatgctcag acggatacgg
    17101 ccctggcgat caacttccag aactttcacc ggtacttcct gacccatctg caggtaatcg
    17161 gtcactttct caacgcgttt gtcagcgatt tgagagatgt ggaccagacc ttctttaccg
    17221 ccgccgatgg caacaaatgc gccgaagtca acgatacggg tcactttacc agtgtagacg
    17281 cggcccactt caatttctgc agtgatctct tcgatacgac gaatagcgtg tttcgctttc
    17341 tcgccatcgg tcgctgcgat cttcacagta ccgtcatctt cgatttcgat ggtggtgcca
    17401 gtttcttcgg tcagggcacg gatcacagag ccgcctttac cgataacgtc tttgatcttg
    17461 tccgggttga tcttgatggt atggatacgc ggagcgaact cagagatatc gccacgtggc
    17521 gcgttgatcg cctgttccat tacacccagg atgtgcagac gcgcaccttt agcctggttc
    17581 agtgcaacct gcatgatctc tttggtgata ccttcaattt tgatatccat ctgcagtgca
    17641 gagataccgt cgcgggaacc cgcaactttg aagtccatat cgcccaggtg atcttcgtcg
    17701 cccaaaatgt cagacagtac aacgtagttg tcgccttctt tcaccagacc cattgcgata
    17761 cccgcaacgg cagctttgat tggcacacct gcgtccatca gcgccagaga cgcaccgcac
    17821 acggaagcca tagaagaaga accgttggat tcagtgattt cagacacaac acgtacggtg
    17881 tacgggaatt tgtccatatc cggcattact gccagcacgc cgcgcttcgc cagacgaccg
    17941 tgaccaattt cacgacgctt cggagaaccg accatgccgg tttcgcctac ggagtacgga
    18001 gggaagttgt agtggaacag gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc
    18061 tgcgcgtcac gagcagtacc cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg
    18121 aacagcgcag aaccgtgagt acgcggcagc acgccagtac gcacatccag accacggatc
    18181 atatcttttt cacgaccatc gatacgcggt tcgcctgcca gtacgcggct acgaacaacg
    18241 tttttctcga tcgcgtgcag aatttcaccc agttcgtttt cgtccagggt ttcgtcttca
    18301 gcaagcagcg tcgcgatggt ttcagatttg atgacatcaa cctgcgcata acgctcttgt
    18361 ttgtcggtga tgcggtaagc atcgctcaga cgagcttcag ccagtgcagc aacgcgcgcg
    18421 ttcagcgctt cgtttaccgg ctccggctgc cagtcccaac gcggtttacc ggcttctttc
    18481 accagttcat tgatgttctg aataacaacc tgctgttgtt catgaccgaa cactactgcg
    18541 cccagcatct ggtcttcgct cagcagttca gcttcagatt caaccatcag tacagcggct
    18601 tcagtacccg caacaaccag atccagtttg ctctctttca gctcgtcctg agtcgggttc
    18661 agtacgtact ggtcattgat gtaacctacg cgggcagcac caatcgggcc attgaacgga
    18721 ataccagaca gagacagtgc tgcggaagca ccaatcatcg cgacgatatc cgggttaacc
    18781 tgcgggttaa cagaaaccac ggtggcgata acctgaactt cgttgacgaa gccttccggg
    18841 aacagcgggc gaatcgggcg gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttgga
    18901 cgaccttcac gacggaagaa gctacccggg atacgaccag cagcgtaggt acgctcctga
    18961 tagttaacgg tcagtgggaa gaagtcctga cctggtttgg cttttttctg gccaacaacg
    19021 gtaacaaata ccgcggtgtc atccatgcta accataacag cggcagtagc ctgacgagcc
    19081 atcatgccgg tttccagagt cacggtgtgt tggccgtact ggaatttacg aacgatcgga
    19141 ttaagcaatg taatatcctt tctctttctt agacagtacc ttacggcact ggtgttaata
    19201 cccgatcttc tgcgcatcct cgcgactaat gacaacccta acccagctct atgtgggtaa
    19261 agcctctcat tagccgcgcg aacctctgca acggaagatc attcatagca acaatacatt
    19321 agtttccagt gaattgctgc cgtcagcttg aaaaaagggg ccactcaggc ccccttttct
    19381 gaaactcgca agaattagcg acgcagaccc aggcgctcga tgagccgggt gtaacgtgct
    19441 acgtctttac gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga
    19501 ccacgacggc tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc
    19561 tgtgcagtca gcagtgctac ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca
    19621 aactcagaaa cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt
    19681 atatagaatg aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt
    19741 gttaaacaat ttacgcgacg ttaagcggca gtattctact cgtagcgacc tgttatcgca
    19801 agacggttaa cattacgccg ggtattcaac caccagacga cgaggcgcaa cgcggccttc
    19861 atcgtcaatt tcgcccatac cgataaactt gccgttctca ccttccgtaa cgcgaaccag
    19921 tccttccagc ggcgcgccag atgtacgaac cgggttacca tttttgaagt aaacagaaga
    19981 cgttaacgga agattcacca ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa
    20041 tggatcaagc aactccgcag ccggaatatc ctgctgttcg gcttgttcga ccagttcacg
    20101 caggtgctcc agggtcacca tccgttcaac cggatattta cttaccgcca gacgacgcag
    20161 gtaaatcaca tgcgcgccac agccgagttt ttcacccagg tcatcaatga tggtgcggat
    20221 ataagtgcct tttgagcagt gaatttccag ctccagctca ttgccttcat ggcgaataaa
    20281 cagcaattca taaacggtaa ttggacgcgc ttcacgcgga acttcaatgc cctgacgcgc
    20341 atattcgtac agttttttgc cctggtattt cagtgctgaa tacatcgatg ggatctgttc
    20401 gatatcgcca cggaaagtat ccagtgccgc cgccagttgt tctgcactaa aggttaccgg
    20461 acgctcttca acgatttgtc cgtcggcgtc agaagtatcg gtacgctgtc caagacgcgc
    20521 aatgacccga tagcgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc
    20581 gaggcaaatc ggcaacatgc cggtcgccag cgggtccagc gcaccggtat gcccggcacg
    20641 attggcgtta tatatacgtt tcactttttg cagcgcatcg ttgctggaca taccctgcgg
    20701 tttatccagc aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag
    20761 tcctccttgc tgtcgtccgg gttaacacga cgttcttcgt catgtttgac cacgctggtc
    20821 accaggtttg acatacgcat cccttcaacc agagagttgt cgtagaagaa ggtcagttcc
    20881 ggcacgatac gcaggcgcat cgctttaccc agcaggctgc ggatgaaacc agaagcttcc
    20941 tgcaacgctt tgatgcccgc tttaaccgcg tcttcatctt tgtcgttgag gaaagtcaca
    21001 tacactttgg catacgccag gtcgcgagac atttcgacac cggaaacggt ggtcatcatg
    21061 cccagacgag gatctttaat ttcacgctgc aggatgagag cgatctcttt ttgcatttcc
    21121 tgcgctacgc gctgcgggcg accaaattct ttcgccataa taaattctcc tgacaaaaaa
    21181 ggggctgtta gccccttttt aaaaattaat ttcaggtgga agggctgttc acgttggcct
    21241 gataagacgc gccagcgtca catcaggcaa tccatgccgg atgcggcgta aacgccttat
    21301 cccgcatgga accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata
    21361 cttcgatcac atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca
    21421 taccgttacg gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt
    21481 catagataac cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt
    21541 cggtaaccat acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt
    21601 ccgccagacc gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt
    21661 tcacttcgtc gatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt
    21721 caatcacttt acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg
    21781 acgccgcagc caaggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct
    21841 tcactttaac ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tcgacagaac
    21901 cctgtacgtc tgccttcagg acgatattca cttcgtgaac ttcgccttcg gtcatgttgg
    21961 cgaacatgtt ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac
    22021 cctgacgata gagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat
    22081 cacccgcagc cggaacaccg gacaagccga ggatttccac cggaatggac ggacccgctt
    22141 ccagcacttc ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac
    22201 acagaacgat atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac
    22261 cacgaccttt atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg
    22321 ctttcagctc cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgataccgg
    22381 tacccgcttt cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga
    22441 tgccgtactg ggagagttcg ttcttaacgc gatccggatc ggcttctggt ttatcgatct
    22501 tgttcactgc aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg
    22561 tctgcggcat tacgccgtcg tcggcagcaa caaccaggac tacgatgtca gttgcctgcg
    22621 caccacgtgc acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca
    22681 tgccgttttc agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc
    22741 cagaggccac tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt
    22801 gacccatgat ggtcacaacc ggagcgcgcg gttcagccgc agctcccgtg tcacggtcgc
    22861 tcattaccgc ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt
    22921 cagcaaccag ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca
    22981 gtttcatcat cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc
    23041 caacggtgat agtttcgccg atcacaacgt cacggttaac ggcctgagca ggcttctgga
    23101 agccttgctg cagcgaagaa cctttacgtt ttccgccctt accgccacgt actgctgcgc
    23161 gtgcttcttc acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg
    23221 ctttcgcgtt acgaccacgg ccacggccgc cttcgacttc acgatcgctt tcgtcttctg
    23281 cctggcgagc atgttgagaa gtagtgacgt gataatcgct ggaatcctca gtcggttccg
    23341 cgttatcagt ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg
    23401 cttcttcttc gagtttacga cgcgcttctt cttcggcttt acgcttgagc tctgcagctt
    23461 cctgctcacg gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc
    23521 tcactttgtc tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt
    23581 gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct
    23641 cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag
    23701 cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt
    23761 tgctttttcc accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca
    23821 atttgtccgg gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg
    23881 cagacacaga gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc
    23941 gttccacgga ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt
    24001 tccttcctgc tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca
    24061 taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc
    24121 cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat
    24181 cacgatctac cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt
    24241 cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa
    24301 cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt
    24361 cttccagcgt cgagaagcct tcctctacca gaacagtcgc gaagtcttcg tcgatgtcga
    24421 gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt
    24481 cgtcaacggt catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct
    24541 gaccgttacg gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt
    24601 gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa
    24661 actgcgccgg gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag
    24721 aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga tcgatacgtt
    24781 tatcgttggt tttcaccgcg attttcgcac gagaacccgg gtcgcgagcc gctgctttaa
    24841 tttcaatcac ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt
    24901 ccggcttgga acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataaa
    24961 gcacgccacg aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca
    25021 ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg tttacttttt
    25081 tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac
    25141 gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggtg gtgatacggt
    25201 caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt
    25261 cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta
    25321 accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga
    25381 tctcttgttc atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa
    25441 tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt
    25501 tgttcatcgc gggcttttca cctcatccag actattaaaa gtggggaacc aagttcgcct
    25561 tctggatatt actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt
    25621 caccgtctac cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga
    25681 gaaccagagt cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc
    25741 gatcgagacc cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat
    25801 ccagcacagc acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt
    25861 cactatcaat atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca
    25921 gttcaaaacc cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg
    25981 tggacaagcc cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca
    26041 gataacaaaa aaccccgata aatcggggct ttatataact gaaccctata accgcaactg
    26101 cggtctggag cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc
    26161 acagtatatt tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga
    26221 cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt
    26281 ggcaaattct actcgttttg ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag
    26341 acgggacgta aaatctgcag gcattatagt gatccacgcc acattttgtc aacgtttatt
    26401 gctaatcacg tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc
    26461 tgctctccct taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata
    26521 aaagatgatt aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc
    26581 aggctcatca tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct
    26641 cccggtaggt caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact
    26701 gctgtggatg cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc
    26761 agacgaagag gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc
    26821 acgtctgatc gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg
    26881 cgcatttcat aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc
    26941 cgtgactggt accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga
    27001 cggtagcacc tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa
    27061 tgctgaactg cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg
    27121 tcgtcatgaa atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga
    27181 aaaagcttac tccacggact ccaacatgct tggtgcaacg catgaagcga aggatctgga
    27241 atacctcaac tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga
    27301 gagcgtgaag atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc
    27361 gttgaacggt aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg
    27421 cggtcgtcac ggcctgggca tgagcgacca gattgaaaac cgtatcatcg aagcgaaaag
    27481 ccgtggtatt tacgaagctc cggggatggc actgctgcac attgcgtatg aacgcctgtt
    27541 gaccggtatt cacaacgaag acaccattga gcagtatcac gcgcatggcc gtcagttggg
    27601 ccgtctgctg taccaggggc gttggtttga ttcccaggcg ctgatgctgc gtgactctct
    27661 gcaacgctgg gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa
    27721 cgattattca atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac
    27781 gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt atcggtcaac tgaccatgcg
    27841 caacctggat atcaccgata cccgcgagaa acttttcggt tatgccaaaa ctggcctgct
    27901 ttcctcctct gccgcttcag gcgtgccgca gatggagaat ctggaaaaca aaggccagta
    27961 attcgacatc aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc
    28021 tttctgagca tcacataatc gaaaacatca tggtttgcat ccacaaccag tgagtctcct
    28081 ttatagtgta caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc
    28141 cacgcattaa taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga
    28201 tccacaccat ccccaagaac ggggctatac cagataaaca tcggaacatg atatgcctgc
    28261 tggctggcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg
    28321 ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga
    28381 cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc
    28441 ggttgaaaca cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt
    28501 aaatgaagaa caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc
    28561 ggcaacaaca attcatcaaa tcctctgaca taaactgttt ccatggcacg catggcgata
    28621 ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga
    28681 aatcctgctt gattagccat attaataatg ttgtcggggt aattatgaat gtcatggctc
    28741 aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcaccgctt
    28801 attgcttgat taaacagttt gatctgtttt ctttgtgctt cgacttgcgg tgtcgtagag
    28861 cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg
    28921 taggtatcaa tacctgtatc cctgactgat aattgaaaat acggcactgt gtttgcaatt
    28981 gagagtaatc tttgatgctc tttcgctgct aaagcaaaat aattgagatt gaaaaacggc
    29041 gtataggtag caaatcgcga cgctagtata tatggactga acgcattttt atttttggca
    29101 tctttataag caaattgaca agcggaaaat aaacttcccg aaatgacaat cagcaataat
    29161 attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac agccaaaaaa
    29221 agaagggata aaaaggcaaa agcacataga taagggctat acattcccag cattttggca
    29281 acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca
    29341 aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt
    29401 gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag gatggtcaga
    29461 acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa gttcgtaata
    29521 ccaaaaacaa taacactaag atacaacaac caatgagatt taaaacttct agcaaatttg
    29581 ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt
    29641 gcaattttat atcagacatg tttttcaact ctgatccata tcagaacgaa acactgttag
    29701 ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg
    29761 cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg
    29821 tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg
    29881 ccaattatgt cagcagtagg gaaaagttta taacgaaggc gtaccggcac catcgtttca
    29941 aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca
    30001 gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg
    30061 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
    30121 ccaattccac cacctcggca cggaaactac tttttagttc gggatatcgc tggtcggctt
    30181 agccggagca gctggctgag tttgttcggt tttcgccggc gcactcaggt tttcccattc
    30241 gctaccttta ttggtcttgt tgctattgat gttacccagc accagactga tgatgaagaa
    30301 taacgttgcc agcaacgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
    30361 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
    30421 tagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc
    30481 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
    30541 gggagtttat aatctttccc actgaagcgg gtgtgaatac taaccaaaac gaatgacctt
    30601 cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
    30661 tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg
    30721 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
    30781 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg
    30841 ctacccagcg ccgtttcaac ttctgcggtc accgctttga ctgattcatg ctccagcgga
    30901 tcgccgctac ctgcggtgta acgcacgttt accaggattt gcgggaacat tttcatgccg
    30961 ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac ctgcaaacca
    31021 gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct
    31081 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
    31141 gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc
    31201 aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
    31261 tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg
    31321 ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
    31381 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg
    31441 atagcgataa cattcgctcc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca
    31501 ccgtttgcgc aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg
    31561 gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt
    31621 tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc ttcttcaact
    31681 gcatccggca gcttagtacc gtcaatagaa aagaatttaa tgccattatc gtagaacggg
    31741 ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc
    31801 acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgcagc cgccagcccc
    31861 gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc
    31921 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
    31981 tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
    32041 cgattactca tagcgtttgt tttcctttgc agacagagta gcttccacca ctcgcatcgc
    32101 ttctacggtt tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat
    32161 gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc
    32221 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
    32281 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
    32341 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
    32401 gtagcgattc acttctgcaa agacatcgtc atacttcggg gcttcctgca tggtttttgg
    32461 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc
    32521 gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc
    32581 acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat
    32641 tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc
    32701 tggccgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt
    32761 cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg
    32821 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc
    32881 aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
    32941 ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc
    33001 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
    33061 ggactaccat tgtcgccagc attgttagaa gcgcctggtt cttcccagcc cgctggcgga
    33121 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
    33181 atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca
    33241 cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
    33301 tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc
    33361 gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat gttacgtgcc
    33421 aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag
    33481 atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga
    33541 cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
    33601 cgtgggataa tcgtcacttt gtgcaccgga tcgtgctccg gcaccaggcg accgataatc
    33661 gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag
    33721 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca
    33781 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
    33841 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat
    33901 ggtacgcgac gcatgtgaac cttcaggatc tgctcacgac cgcgaacatc tggcaagcca
    33961 accacaacct gacggtcgaa acggccagga cgcagcagcg ctgggtcgag aacgtccgga
    34021 cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc
    34081 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc tgcgccacgc
    34141 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc
    34201 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
    34261 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc
    34321 gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt
    34381 ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc ttcttttgct
    34441 tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc
    34501 gctttactct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
    34561 aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc
    34621 agcaggctgg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta
    34681 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
    34741 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
    34801 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
    34861 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
    34921 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
    34981 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
    35041 atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta
    35101 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
    35161 acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga
    35221 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
    35281 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
    35341 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
    35401 caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc
    35461 ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
    35521 atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca
    35581 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
    35641 gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
    35701 ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc
    35761 aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc
    35821 gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat
    35881 tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga
    35941 aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt
    36001 catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg
    36061 ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca
    36121 ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gctatgcggc ctttttcctt
    36181 tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat
    36241 tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt
    36301 gccgatcagg ccacgagcaa taggtgagtt tacagaaatc aggttttgtt taaagtcagc
    36361 ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatcgagat tcagcaccgt
    36421 tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg
    36481 cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc
    36541 acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat
    36601 agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt
    36661 ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct
    36721 gatggggcaa gtgttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg
    36781 gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt
    36841 ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc
    36901 gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt
    36961 tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta
    37021 cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc
    37081 ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta cccagaaagt
    37141 gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct
    37201 tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg
    37261 tgccgatccg acgttaaaac gtcaagatat tcgcaatatg gtcgcgactt tgaaaaaatc
    37321 tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga
    37381 taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgt
    37441 cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga
    37501 tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac
    37561 cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtggtgc caggcgacct
    37621 gaaccgcttt acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt
    37681 tgccgtgcag gatggagcca gctatgccgg tgcaattctg aaagatgagt taaaacaggc
    37741 gggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt
    37801 agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc
    37861 ggacaacatg atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc
    37921 tggaacatgg cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt
    37981 cgatattgga aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc
    38041 ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt
    38101 tatctccatg ctgccgctag cgggctatga cggctctttg cagtaccgtg caggtctgca
    38161 tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa
    38221 cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc
    38281 tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga
    38341 aagccgtttg tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg
    38401 gccggggttt tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt
    38461 cccagtcgtc atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat
    38521 gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga
    38581 ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac
    38641 tcgccgcaga gatcagataa tatttatctt cccagcccag cgcctctgcg atcgctttcg
    38701 ctttctcttc ggcttctacc ttatccagca gatcgatctt gttgaagact aaccaacgcg
    38761 gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt
    38821 caaccggatc ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac
    38881 gttccaggtg cttcaggaag cgaatgccca gacctgcgcc ttccgcagcg ccttcaatca
    38941 gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac
    39001 ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcac
    39061 gaataaaggt cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca
    39121 tcagctccag cagcagctca cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg
    39181 tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa
    39241 ccagcagacg ctgaccgtgt ttggtcatat cgcccatggt ttcaccagta ccctggtcga
    39301 ttacacgcgt acctaccggc actttaattg tgacgtcttt accgcgctta ccggtacagt
    39361 cgcggcttgc gccattctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga
    39421 taagcgtgtt caggttctcg tcggcttcca tccatacgtc accaccatca ccgccgtcgc
    39481 cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac
    39541 cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg
    39601 taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc
    39661 aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat
    39721 aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat
    39781 ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct
    39841 gccagcgagc catcgcttcc gccagggcgc catatcctac caaggtattc agtccgcaaa
    39901 aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag
    39961 ggaagagcgc aattgtacat aaagtgtaca gtaaaaacag gatctgcggt gaggccagcc
    40021 gacgcaataa aaccttttgc gccacgccat aactcaccca aaccgtcgca gcaccgaccc
    40081 caaagataac tccccaggtg taatcggtta gctttgtaaa tatctcgacc agactggtgt
    40141 taaaaaacat caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca
    40201 ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa
    40261 tcacctgcga agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc
    40321 caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc
    40381 gtaatggcgg caacctcttc ttcaccgcaa gaatggcccc caggccaata ctcgccatca
    40441 agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcaa
    40501 ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct
    40561 gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca
    40621 tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta
    40681 ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt
    40741 taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac
    40801 ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca
    40861 gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt
    40921 tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc
    40981 gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata
    41041 gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac
    41101 ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt
    41161 tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc
    41221 caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc
    41281 gtacatataa aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat
    41341 attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa
    41401 tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta
    41461 caatcaccct atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc
    41521 ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa
    41581 gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccaactgatc
    41641 aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct
    41701 gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg
    41761 atcgagttta tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg
    41821 cgcaggggta aagctaccgc caacgccgca tttggcaatg ccgccagcgt gctggtaggc
    41881 gattttattt atacccgcgc gttccagatg atgaccagcc tcggttcact caaagtgctg
    41941 gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaat
    42001 gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg
    42061 cgtctgtttg aggctgccgc gcagtgttcc ggaattctgg cgggctgtac gccggaggag
    42121 gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat
    42181 ttactcgatt acaatgccga tggcgaacag ttaggtaaaa acgtcggcga cgatctgaac
    42241 gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca
    42301 cagatgatcc gtaccgccat cgaacagggt aacggtcgcc atcttctgaa accggttctg
    42361 gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgcgccga ggaagaagca
    42421 gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc
    42481 ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagt
    42541 gcgcgggatg attccagtaa actccataag aatacctatt cagcgtaata atgcactgaa
    42601 aaaccagtgc atacatcttt atatattctg aatattcaca ctctttatag gaacttttta
    42661 gagcaatcgg ccatcaggag tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc
    42721 gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca tcccgctgac
    42781 atcattgcgg gtttgcgcaa gaagggaaca tcaatggcgg cggagtctcg cagaaatggt
    42841 ttgagttcct caacgctggc gaatgcatta tcgcgcccat ggccgaaagg agagatgatt
    42901 attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta ccatgatccg
    42961 cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa accgaaaaaa
    43021 tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga ttattcgcct
    43081 ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg ttcgtatcca
    43141 cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca gccagccagc
    43201 accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga aagtttttca
    43261 acaccgtgac aaataacggt attgctttcg atttcggcgt gcgcgcccat acggctcagc
    43321 tctggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc agtcccttct
    43381 gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc
    43441 gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct aatccagtct
    43501 tcgccgacct cgatgtccgc tccagcgtca cgcagtttcg ccagcacggc gtcgagagta
    43561 tctggctgcg tgttacggca gataattttg ccgcgagaaa tcgccgccgc caccaggaaa
    43621 gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa acgttccaca
    43681 ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag cgtaatcagg
    43741 aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat cgtggtgcct
    43801 tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac tttatccatc
    43861 acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc ttcttccagt
    43921 ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg acgcgcaccg
    43981 atcgtacaac cgccaggtag tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc
    44041 agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca gaatacatta
    44101 acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc cagctggctt
    44161 agcagcttca ttgatgtatc gacgtctttc agtttcggga cgttctggat ctctaccggt
    44221 tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc gccggaaatt
    44281 gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat ttagtttgtt
    44341 ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa gccgttcagt
    44401 ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc atgaatgcgg
    44461 ttatccgcaa tatattccat cagcggacca tagaccgtct gctgtttttt aacccgactc
    44521 atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc gccggaaacg
    44581 tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt attttccatg
    44641 ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt tgcgctggca
    44701 taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag gcaggtttgc
    44761 cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag aacatcagca
    44821 ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg aagcgtcacg
    44881 ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag tccacccgta
    44941 tccacgcggg acacacggct aagatcgatg caggtaatcc ccttcaccgc ttcctcacgc
    45001 atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc cagtgtgtca
    45061 cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga gtgattttct
    45121 gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat acctttggta cgtagcagcg
    45181 ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag
    45241 cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc accggcgggc
    45301 ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatca cccagcggct
    45361 gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct
    45421 gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg ttgagctggg gtcgcactct
    45481 tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt
    45541 cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt tgctcattct
    45601 tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga ttggtctggt
    45661 ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt
    45721 taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca gtggtttcat
    45781 tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg cctttactac
    45841 cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttggtg tcctgaattg
    45901 tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg
    45961 ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg gtatcaggaa
    46021 tatggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt ttcgggtcca
    46081 gcgtaatatc cgccacccgg cccacaacaa cgccaccaat actgaccgga gagcgcgctt
    46141 tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca gtacgtattg
    46201 acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagcagtgct gctaataaaa
    46261 agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc aaacatcaat
    46321 gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac ggtgcgagtg
    46381 gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa caacgaaatc
    46441 cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag atccatacgc
    46501 cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc tttccaactg
    46561 acgccgacca gcgatccgcc ccagatcccc acggcgacga agataaccgt caacagtggt
    46621 aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg atccaccgcc
    46681 atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc tgcggttagc
    46741 gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag ttcacgcagt
    46801 agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt cagaaccaga
    46861 taaccttgca gccccaacac cattccgatg aacacgccag aaactacaat aatcagcatc
    46921 gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt gcgaaattcc
    46981 ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccaaacgt tctcagggtt
    47041 ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg cttaactccc
    47101 tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaaccg gcccgtcagc
    47161 tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg cctgggcact
    47221 gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatccgcaa tacttaacac
    47281 ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt tcagctcaga
    47341 aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag gttcatcaaa
    47401 catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc
    47461 accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg cctccagctt
    47521 catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc gcagtggata
    47581 ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa ataacatgct
    47641 catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat tctcaccatc
    47701 gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt
    47761 tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga
    47821 aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca tatcgactaa
    47881 attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc taagcatggc
    47941 gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg gttagcgaaa
    48001 gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt
    48061 tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag atgcttttag
    48121 ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac cgcctggttt
    48181 ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc ggcatgacgg
    48241 tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg
    48301 aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat atattgctga
    48361 tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttccgatgtt ctacgccgtg
    48421 aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat gacggacaac
    48481 ttagtcgcag cgatggtatc tttctcctct ttctggctgt gctatggctg ctgttcattg
    48541 ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga gagcagcttg
    48601 cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc attgcgctta
    48661 tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg gcgaattact
    48721 ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc agcctgccgg
    48781 aactggcaac cgcaatagcg ggcgttcgca aaggtgaaaa cgacattgct gtcggaaata
    48841 tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg ctgataacgc
    48901 caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg ctggtgagca
    48961 ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt gtaggggtat
    49021 tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg ccaatactcg
    49081 ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt tgactttcag
    49141 caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac
    49201 atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc
    49261 gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac gtttgccagc
    49321 accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga tttaggcatg
    49381 gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag cgaaatcact
    49441 gccttaattc cagtgcttaa gcgtcttcac ataccgttaa tctgcatcac cggtcgcccg
    49501 gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc
    49561 tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat gggcgatgcc
    49621 ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc gctctcacac
    49681 ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat gcatacgggc
    49741 gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct ggaagttacc
    49801 cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga aggcatcttt
    49861 accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt
    49921 gccgatgtga tgacgccggg gggaatacgc gtgcgccctg gcattctggc cgttgaggca
    49981 ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg cgaccattta
    50041 ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag attcaaggat
    50101 aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg
    50161 acgttatagc aaaagcagag aacattcgtc tgctaatcct cgacgttgat ggcgtactgt
    50221 cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg
    50281 acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc attaccgggc
    50341 gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac ttgtatcagg
    50401 ggcagtcaaa caaactgatc gcctttagcg atctgctgga aaaattggcg attgccccgg
    50461 aaaatgtggc ttatgtcggc gatgatctca tcgattggcc ggtaatggaa aaagtgggtt
    50521 taagcgtcgc cgtggccgat gcgcatccac tgttgatccc gcgcgccgat tacgtgacgc
    50581 gcattgctgg cggtcgtggc gcagtgcgag aagtttgcga cttattactc ctggcgcagg
    50641 gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt gggttatcat
    50701 tgtgctatca ctggcggttc tggtgatgat cggtattaat atggccgaaa aagacgatac
    50761 cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata cggacacgct
    50821 cgtctataac ccagaagggg cgctaagcta tcgattgatt gctcaacacg ttgaatatta
    50881 ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg ataaggataa
    50941 aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc ggatgctcta
    51001 tttatatgga cacgttgaag tcaacgcact tgtgccggac tctcaacttc gcagaatcac
    51061 gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag acctcgtcac
    51121 gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact tacgcagcaa
    51181 gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca aacaaactca
    51241 gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca tttgccgtaa
    51301 ccggagacac tgatcagccg atacacattg aatcggacca gcaatctctt gatatgcaag
    51361 gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccata aaaattaatg
    51421 ccgacaaagt ggtcgttacc cgtccgggtg gcgaacaagg taaagaagtg attgacggct
    51481 acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt gaaggtcacg
    51541 cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt aatgcttatc
    51601 tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggtg aaagagcaga
    51661 aaatgcaggc tttcagcgac aaaggcaagc gtgtaaccac cgttctggtg ccgtcgcagc
    51721 tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat taattcgtta
    51781 tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc gtggtagaag
    51841 acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca aacggtgccg
    51901 gtaagaccac cactttctac atggttgtag gcattgtgcc gcgcgatgcg ggcaacatca
    51961 ttattgatga tgacgatatc agtctgctac ctctgcatgc acgcgcgcgc cgcggtatcg
    52021 gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat aacctgatgg
    52081 cggtactgca aattcgtgac gacttgtctg ctgaacagcg tgaagaccgc gcgaacgagc
    52141 tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca ctctccgggg
    52201 gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa tttattctgc
    52261 tcgacgaacc gtttgccggg gttgacccga tctcagttat cgacattaaa cgcatcattg
    52321 agcacctgcg cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg cgtgaaacac
    52381 tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc cacggcacgc
    52441 ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa gacttcagac
    52501 tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga acatgaagca
    52561 aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc aacaggcaat
    52621 tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg cgctggagag
    52681 taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg aaacgcaaga
    52741 cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc
    52801 gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca ccagcggtga
    52861 ctacattgac gacgagctgc cggtctatca gggcgaaacg acgcagacct tgcaggatta
    52921 cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta ttgctacctc
    52981 tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag atattctcga
    53041 aagtatgggc gatgaagaga tcgacatcga cgaagttgaa gccgtcctta agcggatcca
    53101 acggtttgat ccggtcggtg tggcggcaaa agatctgcgt gactgcctgc tgatccaact
    53161 ctcccaattc gataaaacca cgccgtggct ggaagaggcc agactgatca ttagcgatca
    53221 tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc gtctgaaaga
    53281 agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc ccgggcaatc
    53341 gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta agcataacgg
    53401 tcactggacg gtagaactca acagtgacag cattcctcgt ctgcaaatca accagcacta
    53461 cgcctcgatg tgcaataacg cgcgcaatga tggtgacagc cagtttatcc gcagcaatct
    53521 gcaagatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt
    53581 gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag aatatatgaa
    53641 accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg
    53701 cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga agtatttctt
    53761 ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct
    53821 ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac
    53881 ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga agtaccgaga
    53941 gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca accgataagg
    54001 aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag gcactgcgcg
    54061 aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc aaccaggtct
    54121 atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg catgtaaacg
    54181 gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt gatggcttaa
    54241 ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa cactaattgt
    54301 ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca tttgtacggt
    54361 taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa attatgacaa
    54421 ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg
    54481 tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac
    54541 ttagcctacc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa atgggcagta
    54601 ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat actctgcgtg
    54661 ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc gacaaccaac
    54721 cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg cacctgcata
    54781 ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag
    54841 cccagagcga tgaagagctg tatcaaatca ttacggatac caaaggtact ccggatgaag
    54901 cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac atggtactga
    54961 tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata
    55021 tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg gctcgaaccc
    55081 tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg ccggagtcac
    55141 cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg caactactgt
    55201 tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc cgactgcatc
    55261 cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc gatctgctgg
    55321 agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc gttcacgagc
    55381 tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg acaatggtct
    55441 ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc tttgacgtgc
    55501 gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt cttgataagc
    55561 ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac cagacgcgaa
    55621 gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac ttgacggtcg
    55681 ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa ctggcagact
    55741 acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa aaacgtaaac
    55801 catgactgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc
    55861 aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac gtaatgacga
    55921 aggcaccgag gctgaagcca acagcgttat tgcgctgctg atgttggatt ctgccaaagg
    55981 acggcagatt gaagttgaag cgaccggtcc acaggaagag gaagcactgg ccgccgttat
    56041 cgccctcttt aattctggtt ttgatgaaga ttaatcttca tcactttgac atacaactcc
    56101 cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca atcaacgtca
    56161 attgtacatt tcgtgcgctt tttactttca gaagaacctt aagaaaacct taagaagcat
    56221 tgtttaggtt tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg
    56281 agatggcatg attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg
    56341 ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg
    56401 tggcgataaa gagatccgtc gtgagttaaa gtactacgcg catcttggtc gccggttaaa
    56461 agactggagt ggaataccgc gctatcacgg taccgtcgag acggattgcg gaaccggtta
    56521 tgtttatgat gtgattgctg attttgacgg caagccctcc attacgctga ccgaatttgc
    56581 tgaacaatgc cgttacgaag aagacgttgc acaactgcgc caattactga aacagctgaa
    56641 gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg
    56701 tcaccgcatt agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagcac
    56761 attgattcca ctggcgacct ggtcaaagtg gtgttgtctg cgtaagcaag agcggttgtg
    56821 gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg
    56881 tgagagcaaa acgctggccc tcgcttcgcg cgaagcttaa tccagctggt gttgttgcat
    56941 aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctgcgcac
    57001 gtagcccgat ggcgcggaga ctttgaaacg aagtggatta ggtaatacag ctgccagtaa
    57061 ggcagcttcc gatcgggtaa gtttgctcgc gggtttgtgg aaataacgtt gtgccgcagc
    57121 ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg
    57181 ctttttgctc cagacagttt ctatccccag cgttaatccg gcttccagcc cttttcgaac
    57241 ccagctacgc ccatcccata aaaagagatt tttggctgtc tgttgagaaa tcgttgaagc
    57301 accgcgaata cggttttcat tgcgctcatt gtgcgccagg gctttctcaa tggaagcgac
    57361 atcaaagccc cagtgctcag gaaatgtctg atcttctgcg gcaatcaccg ccagtcccat
    57421 ccacggcgag atttgatcca tactgaccct gtcagaatgt gccacgtaac gaaaattacc
    57481 atgcagccag gcgctgacct gtcgctcgac cattaccgca gagaagggaa caggtgcaac
    57541 gctaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcagaag
    57601 aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc
    57661 acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata
    57721 tatgccgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacac
    57781 ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg
    57841 gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa
    57901 ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg
    57961 gtgcattcgc gaccaagact ggcaaaattg cttaaattct ttgccgcgcc aaaccccccc
    58021 ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg
    58081 cgcgttattc gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc ttcgccagtt
    58141 aaatgattga taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg
    58201 ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg
    58261 ccgcatccgc tcagaattac gccaattttc ttcattatga tcatcctttc gcaattgact
    58321 gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact gattcatgta
    58381 acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgcagtgccc
    58441 ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat
    58501 tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag
    58561 cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc
    58621 ttcccaggcg ggaaggtcag gagactgaat ttgctgaccc agctgttgca gatggcgcaa
    58681 ccccactgac cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc
    58741 ctgcgccgtc agattcgact ccagcacgtt gacatagcct ggcatcatct tctcaaacac
    58801 cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg
    58861 aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc
    58921 atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaacag aaagcggctt
    58981 actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca gcacgttagc
    59041 ggttaaggcc accagcggcg gtaaatcctc tcgcggataa cgcttcgtca gttcacgaga
    59101 gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc
    59161 cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa
    59221 tttttccagc acagaacgcg cgacaatcac gttcagttca atgtcttcca ccagcagcac
    59281 attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgctac
    59341 cgacggtgcg tggatcgtca acgtaaaggt tgaaccttta ccctgttcgc tggtaaccgt
    59401 aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc
    59461 ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga agattttatc
    59521 cagctcatcc tgcggaatgc cgataccaga atcttccact tcaaaatgca gcatatcgcc
    59581 ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact
    59641 gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa
    59701 tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca atgcggagag
    59761 attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa gctggacctt
    59821 gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca gcgtgacggc
    59881 cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg tatccagcag
    59941 aatgcggctc aggccgacga taccgttcag cggtgtacgc aattcgtggc tgatggtgga
    60001 aataaacgtc gttttgtcgc ggctggcgcg ttcaagcgca tcctgatacc gcttacgctc
    60061 ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg
    60121 cactttacga atttcaaagc aggctttgcg tccgtcggga taatccagcc actgctcata
    60181 ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt tcgcagcggc
    60241 ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc tttttccggt
    60301 cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac
    60361 caggtcgggt gaagcatcaa ggaaggaacg taaaaatgag gattgctgct cgagctgaat
    60421 ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc
    60481 tgcttccgct ttttcacgaa cggcaatttc ctgatttagc tgggcaatat tatcttttaa
    60541 ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga
    60601 cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca
    60661 cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc
    60721 atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc
    60781 cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc
    60841 cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc
    60901 aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc aacgacctac
    60961 tccgttggag gtagccacgg acgtcccagc gccgatccct gtacgccatg ttcattcaga
    61021 tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagagg agcaagcagc
    61081 gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga
    61141 atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa acgccccgct
    61201 tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag ctttatgccg
    61261 tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg cccttcgcca
    61321 ggcaggccga caattaagtg agaacacacc ttcagcccgc gctgacgtgc cagctgggtt
    61381 gtacgctgat aacaggcaaa atcatggccg cgattaatgc gatgcagggt tttgtcgtgg
    61441 gcggtttgta gccccagctc cagccacact tcgtagccct ggtccttata ttcgcaaagc
    61501 agatccagca ccgcatccgg cacgcagtcc gggcgagtac caacacacaa accgacaata
    61561 ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg
    61621 ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg
    61681 tgcgccagtt gctcggcaat ggaacgatgt tgctgcgctt catcggcaaa tgaggcaaca
    61741 ttacagaatg tgcagccgcc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg
    61801 ccgtgcagcg tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac
    61861 atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa
    61921 gcctgccatt tttagctcaa ttcggcgatg acttggatca atcgtcccgg cctgctttta
    61981 tcaactgcat aaccaatcaa aattaccgaa atttcatgca taatcacata aatcactttt
    62041 gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaac agtagcatga
    62101 aacgtcatta ccaattaagg cagtataaaa tgctggtttt ctcgtcagtt caaggcagga
    62161 taagggttaa cacgccttta tgacagtcag gaattggctg tttctctaac gacgcccctt
    62221 ttagccttaa ttagaaaatc cattttaatt tcagtcaatt aataaagaat tttgcgctaa
    62281 agcacatttc tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg
    62341 gaaatccgct ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga
    62401 gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc
    62461 gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg
    62521 gaagtggggt tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt
    62581 gagagggata actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag
    62641 gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc
    62701 gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc
    62761 atcgttgcgc aggagcgcgg ctggcgttta gcgaaaaact acgccgtcgg gatgctcttc
    62821 ctgaataaag atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agagctgcaa
    62881 cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt
    62941 gaaatcgccc tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc
    63001 tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt
    63061 ctcgaagccg acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa
    63121 ggtctgtgta tgccggcgga tctgccgcgc ttttatctag atcttgcgga cctgcgtctg
    63181 gaatcggcca tttgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg
    63241 ctggcgcaac cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac
    63301 cgccaatggg cgcgtgcgcg tacttataaa ttccagacac cgcttatccc tgacctgcac
    63361 gacgccgcac cgttcgtcaa cgaaatcggc tctgactcca gttcgatgga taacatgctg
    63421 gaactgctgc tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc
    63481 gcctggcaga acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc
    63541 atgcatatgg agccgtggga tggcccggcg ggcatcgtga tgtctgacgg tcgttttgct
    63601 gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct acgtcatcac caaagataag
    63661 ctcatcacct gcgcctctga agtcggtatc tgggattacc agcctgacga agtggtcgaa
    63721 aaaggccgcg tcgggccagg cgaactgatg gtaatcgaca ctcgcagtgg gcgtattctg
    63781 cactcggcag aaaccgatga cgatctgaaa agccgccacc cttataaaga gtggatggag
    63841 aaaaacgtcc gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc
    63901 gaactggacg acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag
    63961 ctggactccg taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc
    64021 gatgataccc cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc
    64081 cagcagtttg cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg
    64141 tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg
    64201 caccgtttaa gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg
    64261 atgaaagagg agcactaccg cgcagatacg ctggatatca cctttgacgt caccaaaacc
    64321 acgctcgaag cgacggtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc
    64381 accgtgctgc tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccggct
    64441 ccaatggcgg ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc
    64501 aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggt
    64561 ttcggcgcga cggctattta tccgtacctt gcctatgaaa cgctgggccg cctggtagac
    64621 acccacgcga ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac
    64681 aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc
    64741 tcgaaactgt ttgaagcggt cggtctgcac aatgatgtag tgggcctgtg cttccagggg
    64801 gcggtcagcc gcattggggg ggcgagcttt gaagacttcc agcaggatct gctgaatctc
    64861 tcgaaacgag cctggctggc gcgcaagccc atcagccagg gcggcctgct gaaatacgtc
    64921 cacggcggcg aataccacgc ctacaacccg gacgtggtgc gcacgctgca acaggcggta
    64981 cagagcggcg agtatagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca
    65041 accacgctgc gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgccgat
    65101 gttgaaccgg caagcgaact gtttaaacgc ttcgataccg cggcgatgtc tatcggcgcg
    65161 ttaagcccgg aagcccacga ggcgctggcg gaagcgatga acagcatcgg cggaaactcg
    65221 aactccggtg aaggtggtga agacccggcg cgctacggca ccaacaaagt gtcgcgcatc
    65281 aagcaggtgg cttccggtcg ctttggggtt actccggcgt atctggtcaa tgccgacgtc
    65341 attcagatta aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac
    65401 aaagtcactc cttacattgc caaactgcgc tattcggtgc cgggcgtgac gctgatctcc
    65461 ccgccgccgc accacgatat ctactctatc gaagacttag cgcagctcat tttcgacctc
    65521 aagcaggtta acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgttggc
    65581 accatcgcca ccggtgtggc gaaagcttat gccgatttaa tcaccatcgc aggctatgac
    65641 ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg caggctgtcc gtgggagctg
    65701 ggacttgttg aaacacaaca ggcgctggtt gccaacggct tgcgtcataa aatccgtttg
    65761 caggtcgatg gcggcctgaa aacgggtgtc gatatcatca aagcggcgat tctcggcgca
    65821 gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg gctgtaaata tctgcgtatt
    65881 tgccacctga acaactgcgc aacgggtgtg gcaactcagg atgacaaact gcgtaagaat
    65941 cactatcacg gcctgccgtt caaggtgacg aattactttg agtttatcgc ccgtgaaacc
    66001 cgcgagctga tggcacagct tggcgtaaca cgtctggtgg atctgattgg tcgcaccgac
    66061 ctgctgaaag agctggacgg tttcaccgcc aaacagcaga aactggcgct gtcgaagctg
    66121 ctggagaccg ccgaaccgca tccaggtaag gcactctact gcaccgaaac caacccgccg
    66181 tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgag
    66241 cgccagagca aaaccttctg gttcgatatc cgcaacaccg accgctctgt cggcgcgtcg
    66301 ctttcaggct atatcgccca gacgcacggc gatcaggggc tggcagccga tccgattaaa
    66361 gcgtacttca acggcaccgc aggccagagc ttcggcgtgt ggaacgcggg cggcgtggaa
    66421 ctgtacctga ccggtgatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc
    66481 gccattcgtc ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac
    66541 acctgcctgt atggagcgac gggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt
    66601 ttcggcgtgc gtaactccgg tgcaatcacc gtggtagaag gcattggcga caacggctgt
    66661 gaatatatga cgggcggtat cgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg
    66721 ggtatgaccg gcggcttcgc ttacgttctc gatgaaagcg gcgatttccg caaacgcgtt
    66781 aacccggaac tggtggaagt attaagcgtt gacgatttgg cgatccatga agagcatctg
    66841 cgtggtctta tcaccgagca tgtgcagcat accggttccc agcgtggtga agagattctg
    66901 gcgaactggt caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgtc
    66961 aaagcactgc tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa
    67021 ggggtagcaa caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg
    67081 ccaaagaaac cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc
    67141 gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag
    67201 tggaaatgcc cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt
    67261 atttttgaag cggcagaact gtcgcaccag accaacactc tgccggaagt atgcggacgt
    67321 gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc tgaacgatga gtttggcgcg
    67381 gtgaccatcg gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt
    67441 ccggatatgt ccggcgtgaa acagaccggt aaaaaagtgg cgattatcgg cgcaggtccg
    67501 gcaggtctgg cgtgtgcaga tgtcctgacg cgcaacggcg tacaagcggt cgtcttcgac
    67561 cgtcacccgg aaattggcgg cttgctgacc ttcggtattc cggctttcaa actggaaaaa
    67621 gaggtaatga cgcgccgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat
    67681 accgaagtgg gccgcgacgt acagctggac gatctgctga gtgattacga tgccgtgttc
    67741 cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc
    67801 gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggcgaa
    67861 actcgtgaag aaccgttcgt cagcatggaa ggcaaacgcg tggtagtact tggcggtggc
    67921 gataccgcaa tggactgcgt gcgcacgtct gtacgccagg gggcaaagca cgttacctgt
    67981 gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg
    68041 gaagaaggtg tagagttcaa attcaatgtc cagccactgg gaattgaagt gaacggtaac
    68101 ggcaaagtca gcggcgtaaa aatggtgcgt actgaaatgg gcgaaccgga tgccaaaggc
    68161 cgtcgccgcg cggagatcgt ggcaggttcc gaacatatcg ttccagcaga tgcggtgatc
    68221 atggcgtttg gtttccgtcc acacagcatg gaatggctgg caaaacacag cgtcgagctg
    68281 gattcgcaag gccgcatcat cgccccggaa ggcagcgaca acgctttcca gaccagcaac
    68341 ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg atctggtcgt taccgctatt
    68401 gccgaaggtc gtaaggcggc agacggcatt atgaactggc tggaagttta agctctgtcc
    68461 cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct cctttcgttc tggaaagcgc
    68521 ctcgcataat ctgaagaaaa ccaatgtaat acgcacatct ggattgttac cggcagatgc
    68581 ccggcgtata gctgattcca ttattcaaca ctgcgccata taaagggaaa gataatggaa
    68641 tcgctctcag agagaacctc aacaggctac cagcaaatcc acgacggtat tattcacctg
    68701 gtcgatagcg cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac
    68761 tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat
    68821 ggtgcgcagc taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc
    68881 tctgcaaaaa acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt
    68941 cgccagacag tgtctggcga attaacacca ttgccctggt ccacttacgt ccgtttactt
    69001 tccgttaaaa acgctgacac ccgcagcttt tatgaaaaag agacgctccg ctgtggctgg
    69061 tctgttcgcc agctagaacg gcaaattgcg acccaatttt atgagcgaac actgctgtca
    69121 catgacaaat cagccatgct gcaacaacac gctcctgccg agacgcatat tcttccgcaa
    69181 caggcgatac gcgatccctt tgtgctcgaa tttctggaat tgaaagatga atactcagaa
    69241 tccgattttg aggaggcgct gatcaaccac ctgatggatt tcatgctgga acttggggat
    69301 gattttgcct ttgttggtcg gcagcgaagg ttacgcattg atgacaactg gtttcgtgtc
    69361 gatctgctgt ttttccaccg ccgtttacgc tgcctgctaa tcgtcgatct aaaagtgggc
    69421 aaattcagct atagcgatgc cggacagatg aatatgtatc tcaactacgc caaagagcac
    69481 tggacgctac cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga gaaaggagtc
    69541 ggagaagcgc attatgcact ggctggtttg cctaacaccg ttctggcgag tgaatataag
    69601 atgcaactac ctgatgaaaa acgactcgcg gatgaactcg ttcgaacaca ggcggtgcta
    69661 gaagaaggct atagactccg ttaatttcag cggtttcgaa ttctgcagac actgtctgca
    69721 gaatctttaa aacgaactaa gttttaagcc attaaatcag ccttaacctt caccaccacc
    69781 tttttaatct caccaggttc gccgacaacg catcctggtt tatgcggttc tcccggcata
    69841 aacacggcga acattcccgg ttttaagttg atggtttgct cgttttcaat ggcgctgcaa
    69901 agctggtaat catcttcatg gtggaactct tcacactggc gcgcagtgcc tgccgtgcca
    69961 aacagaatcc gttcctcacc ttttaataac agctggatat caatgtattg ctcgtgcaat
    70021 tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaaaatattg
    70081 tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc taatgccagc
    70141 gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc
    70201 atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcatggc
    70261 ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt
    70321 ccaccagcgc cagataccct tctgccagac caacgctgcc accgaccacc acgcactgac
    70381 aatcagttgt ggctttaata tcagcgatca gccttgccag cacatgtgcg gagcggtgaa
    70441 tcagctgctg cgcctgctcg tcgccctgcc cggcgcgcat gaaaatagtt ttcgcatccg
    70501 cgccagccaa ctccccctgc gctgccgctg caataccgcg accagaggca atcgcttcca
    70561 cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc gtatgcccga
    70621 tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc
    70681 cgacgccagt ggaaacggtg ataaacacca tatcggttat atcgccttcc agcgcctgat
    70741 actctgccca tgctgcggcc tgggcgtcgt taatggcaat ggtcggcaaa tcggtaagtt
    70801 gccccagcgt tttgactaac ggaaagtgta gcaatccacc aagattatgc ggattcagcg
    70861 ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag
    70921 cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg
    70981 ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg
    71041 caagtttagt accgccgata tcaatcgcca gtgtggtcat agcaccgcct ttttcatcgc
    71101 tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc
    71161 ccacgcgccg tggcgcatcg catcagccgc ctgagcaggc gtgttgtaac gcccttcggc
    71221 aatcacccga catccggcgt cgctcaacct tttcaccagc gtcagatccg gctcttctgg
    71281 cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg
    71341 gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatgatg
    71401 aatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc
    71461 gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg ccgtgatgcg
    71521 taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc tcaccaccgc
    71581 acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc ccgcctgctc
    71641 tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt ccggaaccgg
    71701 ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa
    71761 cgacatacat cttcccttag cgaaaggccc gatacagaga ccgggcaaca ggattaactt
    71821 ttggttttga ctaaatcgtt tttggcgctg ccaaacggca cggcaccgct aaatggtttg
    71881 ccgtcgatag cgtcatgagt acgcaacgct tccgggcgca gccaacgctg aacacgagaa
    71941 ggcatatcca gaccaatcag caggatcacc acaaatgtca gactgaacga gagcgatgcc
    72001 agtgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg
    72061 ccacccaatg cgccaacgtt ataggtaaag cccaggcccg ctgcacgctg gtcggtatcg
    72121 aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg
    72181 aagaacagta acagaccgag cacccagacg tttgcaccgc caatagcaaa taccggaata
    72241 atcagcagct gcgaggccaa cagactacaa acgtacgctt tgcgggttcc cagccagtca
    72301 ccgaggaagc caccgacgca gcaccccacc gccgcgccaa agccactaaa gaacagcaca
    72361 ttggcgacag tatgagggtt ataagccaga tcggttttca gatacgttgg cagcagcgcc
    72421 tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgccagtt
    72481 ggccagcgtt tgcccgtact ctgcaccata aagctgataa agattgcggt gcataacagc
    72541 ccaagaacag cgacgatcgc ggcattttgc aggttaccgg cgaagcagaa ccacagcgca
    72601 gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata
    72661 tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc ttctgcttcc
    72721 gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc
    72781 gcacgccagc cccagaccgg aaccaccagg ctgtagacct gagcggcaac gacggcccct
    72841 acagagaagc ctgaaatcag aaagccactg gctttgttac gcagatgttt tggccagctt
    72901 tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga
    72961 cgagcgataa acatggtgat gtagcctggc gcaaagccgc atgccagcgt cccggccgag
    73021 aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg
    73081 agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc
    73141 accgtcgtca gcccgaattc accttgtact tcggtgagta ccagggcgat taaaacgaaa
    73201 tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcccgcca ttgtgcacga
    73261 ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg
    73321 tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt ttatttcccc
    73381 tcacccggta ggggcgagcg aggggaaaca actcacccgc gctcttgcat caactgctgg
    73441 gccagcgcct tcagttctgg cagatatttt tcatctaccg gaccaaacgg tttgcggcac
    73501 agcggcacag aaacgacatc catataatgc agaacagttt tcaggccgcg gaatacaccc
    73561 gttttgatca gtaaatcaat gactttgttg cattcagttt gcagtttctg cgcggtctgg
    73621 atatcgcctt ctttcagcgc cttaacgatc ccctgatagc gccagcccat gatgttgtag
    73681 gtactaccga taccaccatc agcgcccgcc agcaggccag aggcgaagat ttcgtcgtaa
    73741 ccgttataga gcaccagatc aggatgttca cgacggatct gctccatctg atagagatcg
    73801 ccagaggtct gtttcagcgc acctacgcca ggcaatgtaa caagtgtgtt gatctgatcc
    73861 agggtcagtt ttaccccact cagggctgga atgttgtaca ccaccatcgg caaaccatcc
    73921 gccgaatcaa taattgcccg atagtgatcg cagtgttctt caaagctgaa aggatagtag
    73981 aacggcgtga cggcggagac ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt
    74041 tggctttcgg cggtgctgac gcaaccgacg tgggcgatga gtttaatctt acctttcgcc
    74101 tcttcggcga cgatttccag tacctgttca cgctcggaaa ggctttgtac aaaggcttcg
    74161 ccggtcgaac cacccacgta taaaccgtca atgccctgct gaatattgaa ctgaaccagg
    74221 cggcgcagac tcgctttatc cagtgcttgt tgttgatcaa aaggagtcag gagtgcagcc
    74281 attacgccac gtaaattcgt tgccataaat acctctgaag tgatgcttgt ctgataaacg
    74341 atataccttt atacctgtta taccagatca attaagcaac accccataca gaaagcttat
    74401 aatgcgatct gcttcactaa agtggcatta tttctttttg ttggtggtct gaccgaaggc
    74461 gtgccaggta gcagagacgc tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg
    74521 atcgtggcgg cggatcgcgt caacgatcgc aatatgctgt tgatagctaa cgttgttatg
    74581 ctcgtgcaga gcctgatcag ccacggttgg acgtgcggcg atcagccagt cgagcagggc
    74641 aacgtggatc gccatgaaga ttgggttacc ggggatctcc gccagcacgc ggtggaaatc
    74701 aacgtctgag cgaatgaatg ccgcgttgtt atccagcgac tgactgttga tttccagtgc
    74761 ttttgccagc aaatcgattt gctcatcggt ggcgtgttca gccgcatagc gcaccaggct
    74821 ggattcaaag aacagacgta attgttcgaa atgggcaatc ccaccaggat gagaaaggaa
    74881 atctttcgcc atgccggaaa gctcgccgat gatggtgtcc gcagaaggac gtgagacgcg
    74941 ggcacgttcg ccgttgttta tttgcaccag acctttgcgt tttaacgctg ccagcgcttc
    75001 acgcaccgaa ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag acggtaattg
    75061 ttcaccttcg ccaaattcac gacggcggat catctgttcc agctcttctt ccaccatttc
    75121 ggagagtttt ttacgcgcca gcgggcggct acgcaagttg cgaccaattg caggtgaaga
    75181 atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta
    75241 acatcatcag tgacatccta tcacaggatt gaaagtaggg gaaaatggca gggttttctc
    75301 tttgtgcctc atcattacca taattaacgg aataattaac tattgcgaaa aattaatgta
    75361 acgcagataa aaacatcccg tttgaattat ttataagact attcacgagc attatgaata
    75421 ttatgaatgt gttcttacaa aataatcata agcgcatatt ttttaatgaa aaatcacctc
    75481 acctacaatt aaaaacacga catccgcacc ataaatagac ttgcaaaaaa tataacatca
    75541 ttgttttcaa tctgccgttt atgggattga cccttttctt ttgacacgga gttcaacaat
    75601 gttcggcata attatatctg tcatcgtatt aattacgatg ggctatttga tcctgaaaaa
    75661 ctacaaacct caggtggtgc tggctgcagg tatcttcctg atgatgtgcg gtgtctggtt
    75721 agggttaggt ggtgtactcg atcccgccaa aagcagcggc tacttgatcg tcgatattta
    75781 taatgaaatc ctgcgcatgc tgtccaaccg cattgccgga ttggggctgt cgattatggc
    75841 ggtgggcggt tatgcccgct acatggagcg cacaggagcc agtcgcgcga tggtgagctt
    75901 gttaagccgc ccgttaaaac tcattcgctc gccgtatatt attctgtcgg caacttacgt
    75961 catcggccaa atcatggcgc agtttattac cagcgcctcc ggcctgggta tgttgctgat
    76021 ggtcacctta tttccgacac tggtgagtct gggagtaagc cgtctctctg cggtggcggt
    76081 tatcgcaacc acaatgtcca ttgagtgggg gattctggaa acgaactcca tttttgcagc
    76141 ccaggtagcg ggaatgaaaa ttgccactta cttcttccac taccagcttc cggtcgcctc
    76201 ttgcgtcatt atctcggtgg cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa
    76261 agataaaaat atcaatcacg aacaggcaga gcaaaaagct ctcgataatg tcccgccgct
    76321 ctattacgcc attttacctg tgatgccgtt aatcctgatg ctcggctcgc tattccttgc
    76381 ccacatcggg ctgatgcagt cagaactgca tctggtggtg gtgatgttac tgagtttgac
    76441 gatgtttgtt gagttcttcc gcaagcataa cttgcgcgaa acaatggacg atgtgcaggc
    76501 gttttttgac ggcatgggta cgcagtttgc caacgtggta acgctggtgg tcgcgggtga
    76561 aatatttgcg aaaggcttaa cgacgattgg cactgtcgat gcggttatca ggggtgcgga
    76621 gcattctggt ctgggcggta ttggcgtgat gattattatg gcgctggtca ttgccatttg
    76681 tgccattgtg atgggctctg gcaatgcgcc gtttatgtca tttgccagtc ttattccgaa
    76741 tatcgcagcc ggactacatg taccagcggt tgtaatgatt atgccgatgc attttgccac
    76801 gacgctagcg cgcggtttcg ccgattactg cggtggtggt cgttacgtca ggaattgcag
    76861 gcgtttcgcc ttttgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg
    76921 tgaatatgat tgccacaatc acgctatttt attaagtcat taaaagacaa aacaggccgc
    76981 cagggcctgt ttttgtatta cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc
    77041 tgcggtggtt catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata
    77101 accgacataa cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct
    77161 tcatcgtagg cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata
    77221 gccagcacgg cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta
    77281 aagcgcacct catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt
    77341 acgatttgcc cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc
    77401 accaccaggt gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga
    77461 tagggacgac gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca
    77521 tttcacgttc tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca
    77581 tatagccttt cagctctttc gcacccgggc cgctgaactc gatacccagt tgcggcagac
    77641 gccacagcag cggagcaagg tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg
    77701 gcttctgacc gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg
    77761 catctgcttc agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt
    77821 cgatgcgatg catgtacaaa cggctttcac cgcgagctac cgggtaaaca ggcatcagtg
    77881 gcggatgcgg gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca
    77941 gctcacgatc caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag
    78001 gcggattgtc cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa
    78061 tgcggacctg atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt
    78121 tgttggcagc gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc
    78181 caccaggtgg ccagtcagaa gttgtgttac ccagtaagga acgactctct ttgttcgaaa
    78241 atcaaacaaa aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca
    78301 gattttgcga cctttgtggt gagtcgatgc cggaaatggg gaaaaaaaga tgcgctttag
    78361 tctgaaatag ttgacttagt cccttattgg cgatgtggtt tttgttttgc ctgtctgtca
    78421 gacggcagca aaaagcaact ttccattttt tacgctgatt cagaatttaa ccataaaaaa
    78481 acccgccgaa gcgggttttt tcgcaaattg ttttctgccg gagcagaagc caattaacgt
    78541 ttggagaact gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga
    78601 cgagcgtcac gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat
    78661 tccatcagag cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca
    78721 cctttaacgg tgatgtacag gtccagtttc tcaaccatat cgaccagttc cagcggctga
    78781 cgaactacca tgcgagcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt
    78841 acgattttac cgttgcccgg tttgatgaaa acgcgagctg cggaactttt gcggcgacca
    78901 gtgccgtagt attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc
    78961 ggttgctgtg ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata
    79021 gcacgaccca gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca
    79081 ggacggcgag caatcatctc ttcaaaggtc gcttgtttga taccaccgat gtggccggtg
    79141 tggtgatagt acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc
    79201 agaacgatga tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg
    79261 cgcaggcgac gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca
    79321 acataccagt cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta
    79381 cccaataaat agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac
    79441 gacaaagtcc ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg
    79501 gaaaaaaact ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc
    79561 gacccctttt tgtgatttgt ggcaggtttt aacccgctaa atgctcgcgc ttcagatact
    79621 cttcgctttg catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat
    79681 cgccctgata aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct
    79741 cgtaaaactc atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca
    79801 taactggtac atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat
    79861 gctgactgcg ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga
    79921 cgcccattgt cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc
    79981 cccccgccag cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg
    80041 aaagccacag atgcgcctga gtgagtgtac gcagacgata atcaacacca gcgtccacgt
    80101 tcattacatc acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca
    80161 agccatttcg ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc
    80221 gggcgaacag ggctttcatc aggccgccaa gtagcatggc atcggtaata tcagagacaa
    80281 aaaattcgtc aaaacagagc acgtcagttt ctgctttaaa acgatcagca ataatttcca
    80341 gcggatcggt ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt
    80401 gaaagtgcag gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc
    80461 aggttttccc gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg
    80521 tgtcttcccg tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg
    80581 ctggtggcgt gctattgatg agttcctgat aaataatttc caagcggctg acggcctctt
    80641 tttgaacgtc gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg
    80701 gagtaacgct ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac
    80761 ggttgacgaa aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt
    80821 ggaattaacg gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct
    80881 acggaaacaa aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag
    80941 tcgtcggcat cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc
    81001 aacaggcgtt gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag
    81061 agctggttag ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc
    81121 gccagctgta tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg
    81181 aagcaaaccc gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg
    81241 tgcagatgcc tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc
    81301 gcgactaatt tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact
    81361 atcatttaat ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt
    81421 tgaatctctt ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaacccagc
    81481 tgttgagtgc attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgttg
    81541 cgtcgatccc aggccaggtt gccgatcagg cccccctccc cagtctggct ccaatgctgg
    81601 aaaaagtgct tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga
    81661 aaatcccgga agaattcaaa aagttttttg gtgatgattt accggatcaa cctgcacaac
    81721 ccttcgaagg tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca
    81781 acaaccatgt gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt
    81841 ttgatgcaaa actgattggt agcgatgacc agagcgatat cgccctgttg caaattcaaa
    81901 acccgagcaa attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt
    81961 ttgccgtggc ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt
    82021 ccgcattagg acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg
    82081 cttccattaa ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg
    82141 gcatcaacac tgcaatcctt gcgcctggcg gcgggagcgt cggaattgga tttgccatcc
    82201 ccagtaatat ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg
    82261 gtttgttagg catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg
    82321 acgtgcagcg tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg
    82381 gcgtcaaaac gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg
    82441 agttgcgctc tcgtatcgcg accaccgagc cgggcacgaa agtgaagctt ggcctgctgc
    82501 gtaacggcaa accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca
    82561 gcgctgaaat gatcacgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag
    82621 atggcggcaa aggtattaaa atcgatgaag ttgtcaaagg aagcccagct gctcaggctg
    82681 gcttgcaaaa agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg
    82741 aaatgcgtaa agtgctggcg gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca
    82801 atgaaagcat ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg ctgacgtgtg
    82861 atgtccggtt aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat
    82921 gtttgtgaag ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt
    82981 tgccatgcct tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga
    83041 tgagacgcct gccagttata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt
    83101 ttacaaccgt ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc
    83161 cggtgtaatc atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc
    83221 cgatcagatc atcgtcgcct tacaggatgg acgtgtattt gaagcattgc tggtgggatc
    83281 tgactctcta accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat
    83341 tccaattaat gcacgtcgcg taccgcacat tggcgacgtg gtactggcga tcggtaaccc
    83401 gtacaacctc gggcagacca ttacccaggg gattattagt gctacgggtc gaatcggtct
    83461 gaacccgacc gggcggcaaa acttcctaca aaccgatgct tccattaacc acggtaactc
    83521 tggcggcgcg ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga
    83581 taagagtaac gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc
    83641 aaccaaaatt atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggtat
    83701 cggcggacgc gagatcgcac cactgcacgc gcagggcggt ggtatagatc aactgcaagg
    83761 gatcgtggtt aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa
    83821 cgatctgatt atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca
    83881 ggtggcagaa attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca
    83941 gttaacgctg caggtcacca ttcaggaata tccggcaacc aattaaggcc agcgtgaaag
    84001 acaaaaaaac cggagtctgt gctccggttt tttattatcc gctaatcaat tacttattaa
    84061 cgaactcttc gcccagggcg atatctttct tgagcgtatc cagcatacct tccagcgcgt
    84121 tctgttcaaa tgcgctcagg gtaccgatag atttacgctc ttccacgccg tttttaccca
    84181 gcagaagcgg ctgagagaag aaacgggcgt actgaccgtc gccttcaacg taggcacatt
    84241 cgacaacgcc ttgttcgccc tgcagtgcgc gaaccagaga caggccaaaa cgtgcagcag
    84301 cctggcccat agacagggtt gccgacccgc caccggcttt cgcttcaacc acctcagtac
    84361 ccgcgttctg gatacgtttg gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag
    84421 gaacctgtga caacagcggc agaatggtaa caccagagtg accgccaata accggcactt
    84481 caacttcgcc tggctgtttg cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat
    84541 ccagcgtggt aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt
    84601 cagcagcaat cgcaactgtg gtgttaaccg ggttagtgat aataccaatg cacgctttcg
    84661 ggcaggtttt cgcaacttgc tgtaccaggt ttttcacgat gccggcgtta acattaaaca
    84721 ggtcggaacg atccataccc ggtttacgcg ctacacctgc agagataaga acgacatctg
    84781 cgccttccag cgccggagtc gcatcttcac cagaaaaacc tttgattttc acagcagtag
    84841 ggatatggct cagatcgacg gccacaccgg gagtcactgg agcgatatca tacagagaga
    84901 gttctgaacc tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc
    84961 cagcagcgcc gaggactgcg actttcatcc taaactcctt attatattga taaactaaga
    85021 tatgttgctc cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct
    85081 caagaatgtg tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc
    85141 taagttacgt aattaacaat cgcttatgca ttagcgcaac attccaacag gtggtgacaa
    85201 tataccctac cgttcagcca aaacaacatc aatttgataa caattaattt acttttaagc
    85261 agaatttgca tgccgtgacg caggcatatt tctcaataac gaaatttgat aaaatcccgc
    85321 tctttcataa cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg
    85381 tatgcacaat aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag
    85441 caagaagaac tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag
    85501 ggcgaaatcg tcgccgcgtt gcaggagcaa ggctttgaca atattaatca gtctaaagtc
    85561 tcgcggatgt tgaccaagtt tggtgctgta cgtacacgca atgccaaaat ggaaatggtt
    85621 tactgcctgc cagctgaact gggtgtacca accacctcca gtccattgaa aaatctggtg
    85681 ctggatatcg actacaacga tgcagttgtc gtgattcata ccagccctgg cgcggcgcag
    85741 ttaattgctc gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct
    85801 ggcgatgaca ccatctttac cacccctgct aacggtttca ccgtcaaaga cctgtatgaa
    85861 gcgattttag agctgttcga ccaggagctt taatctctgc cccgtcgctt ctggcggcgg
    85921 ggaaaatgtt gcttatccct ctcaaccccc tgctttcccc tgcgattaat ttaacgaata
    85981 gtgcgtttta ctgcgacatg tcattcacac aatgaataca taaggtaaaa aaagcacatt
    86041 atgcaaaatt cattatctaa ttgaaaaaac tagaattaac gataaataac cgtattttta
    86101 attctttttt gttattaaaa ttcacatttt taacacttag tatcaactaa aacagttagc
    86161 gcggtattaa ttagctcaat aattagtgta tacttgattt tgtgatatgg gtcacgaaac
    86221 aaaggcccag ctaaaagatt atgtcgaggt aaaaatcatg aaaatcaaaa ccactgttgc
    86281 tgcattaagc gtactttctg ttctctcttt cggtgcattc gctgccgact ccattgatgc
    86341 tgcacaagca caaaatcgtg aagcaatcgg gaccgtatcc gtaagtggtg tggcgtcttc
    86401 gccaatggat atgcgtgaaa tgctgaacaa aaaagcggaa gagaaaggcg caacggccta
    86461 ccagattact gaagctcgta gcggtgacac ctggcacgct acggctgaac tgtacaaata
    86521 aaccctcatc gtcttgtccg acgatattgc ccccggttcg ggggcttttt tttgcgctaa
    86581 tgacgaacat taaaacgcaa atgcccttcc agctcttcct ctgcctcatc aaacagcaat
    86641 attaacgcgc caaaacggcg acgcgttttc tctcccagat gaacaaattc aatctcaagt
    86701 ggtagcggca ggacatcgtt cattaacaca tcccatagtg agtcgagatc gcgtacctta
    86761 tctttcgcca gaccaaaggt ttggctaaag tcacgataaa aatcctcctg actctcaatc
    86821 tcatcaaaat caaaggtata aatattcatc tgttgccacc gtcacgtttc gcggcgagtt
    86881 ctgccgtgca tcgctaagta tagccatgaa aaaaccgacg cttttggcgt cggttttgac
    86941 ttaactatcg gtcaacgcat gttgatactt atggagcatc cccgccagcc gatgaaccgg
    87001 ttccgtcacc tgcggtggcg cttgccagat gcatagcttt tcctggtaga tttccagttc
    87061 ttccagcaac tgaccaaagt accgacgacg tttatcatcg ctacgggcag atatcacatg
    87121 gtctgctgtg cggcgcattt gtcggtgaaa cgccgataaa tcctcgttaa ccgggatcgg
    87181 cgcatcacgc aggcgctggt gggcgataat catcgtcagc gccaggcgaa atttcggcaa
    87241 atcccctggg aacttattca tcagcaaaaa cagctgctga taaagtgccg ggaggtggtt
    87301 ctctttacga cgtgccacat tggtagtcat cgcggaaaca gcggcagaaa caaactgatt
    87361 aagcagtaca cgtccagtcc tgtcgcgcga tttatcccgc accagcaaaa taacggtgaa
    87421 cgcgagcaca cagccgacga tttgccctaa tgcgctgtcg agaaactgac tgaaatggaa
    87481 agtcatcggg ttatccagca cgataatatt tatggtgctg gccagcgccc ccatcgagcc
    87541 cagtcgccgt ttctgtactt ctattccgag gaagaatccc agcactgcca ggctaataca
    87601 cagcagcaac atgctctgtt gggtattagg gataatcacc aaaaagtaga gcagccctaa
    87661 cggcagcgcg gccagcgtcc cgtagataaa gtcgatcgcc accatgcgtg gattcggcaa
    87721 acgcattgcc agtgacgtca cgaccgcaat catcaccatt gcaccactgc cggaagtcca
    87781 gcccgtccac agccagaaaa gcgttcccag aatgcaggaa agtgtggttc gccagaagtt
    87841 aaccatcgca tgatgacgtt cggctgactc tactttgact tccggttcgc cttgcaggat
    87901 ctcttcttcg gtggcgttga tttttgtgtt actgataacg ccgcgcttga gaagctgata
    87961 acgcgttgcc gccgcgaccc agctataaat ggtgacaggc gtttcccgtt ccccggtcca
    88021 ggcgataact cttcgcaggc gtttgagctg cttgtggacg tcctgcgcgg tttctaccgg
    88081 cgtgtcaaaa aattcgcgga aagtatcagt gatcaattcc gggcgcgtat tctgaataag
    88141 ataagtttcg caggattggg taatcagcgt cagcgatagc gtattgatcg cttttaaacg
    88201 tcgattggcc cgcgcccagc gggaagattc catattcagg ttgctgcgca tgccttgtag
    88261 cgccgtggtg cgtcgcacca ggtcgcccca ggctttatcg acaacttcac catcgccatg
    88321 cttgatacag agttgcatta attgatattg cgcgaccagc aaactttcca gctctcgatc
    88381 cacttcttgt ttgatcgatc gcggagaaaa gagcaaatcc gccataatcg cacacacaat
    88441 accgataacg atctcgctac aacgttcgac ggcaaactgc ggcgtaagca atggttccgg
    88501 ctgaatggtg atcacaatga tcagcgcggt ataaccggcc agcccccacg catacgagtt
    88561 ttctattcgt accagcgagg atatccaggt acaaaaaccg gcccagatac agcacaccag
    88621 aatcatcaat aatggtgcgc ggatcatcgc aatgatgatc accagtccgg caatacagcc
    88681 aataaatgtg ccgatgatgc gcaaaaagcc acgatagcga atagcgccag aatacggttc
    88741 acctcccgca gcaaaggccg gaccggcggc aacaatcgcc gctgtcagta ccgcccagcg
    88801 tggcgtttcc agctggaagt gaaagccaac aaacagcgcc agtacaatgg cggtcgccag
    88861 ttttaccgca aagcgaatat gttggttagc aatggagaaa atatccatcg tgattaacca
    88921 aactcacgca ggcgatgggc cattttacgg aagaacgaat cctggctttc gtcgcgatct
    88981 tgtttgccag tgaccaccac tgtagcagtg gtgcccgcag gccagatgtt ctcttgctgg
    89041 ttgtcgagac gaatacgaac cggaacacgt tgcgccaaac gcacccattc aaggttagag
    89101 tcgatggtcg ccatcccttt gtcgtcacgc gtgctgctgg cgttggtgac cccagcggca
    89161 acactatcaa cagtcccttt cagcactttg ttactgccaa gcggcgtgat ctctgcacga
    89221 tacccagggc gcaccccttc cagcttagtt tcttccatat aggccagtac atagaaagag
    89281 ttctgtttca ccagcgcaac cgccgttgat ccacgagtaa taaactcacc ggtatagacg
    89341 ttgaggttgg tcacccagcc atctgctggc gcgcggatca ccgtgcgttc aagatccagt
    89401 tttgccagat cgcgggtcgc ctgcgctttc gctaactgat gcagaaccgt ttgtagtacg
    89461 ttgttggcct ggtcgatctc ttcgcgagac atcgcctgca caccgagacg gttacgacgt
    89521 ccggcctcct ggcgtttctc ctgtgccagt acctgataat aagcaacatc ggcttgcgct
    89581 tcctcaagcg ccttttgata gcgcggctgg tcgatggtga acagaacctg tccttttttc
    89641 accagctggt tatgaacatt cacctgggta atgagtccag aaacgtccgg cgcgatcgca
    89701 acgacgtcag cgctaaagcg cgcgtcacgc gtccaggggg attcggtgta atagacccag
    89761 gcattaaaaa ttgcgatgaa ggccagaatg actaatacga ccgtgatggc cgtacgggag
    89821 aattttctta ttagtgtttt cacttcaacc tcaaacgaac agtcgcgata tcaaataaaa
    89881 caagcagcaa tagagcgcgg tgttgaacaa cgccggatgc cagacaaagt cgtagatacc
    89941 tgttggcaca agtacccggc acaccagcca gaaaatcgcc agtgataaaa gcaattcaaa
    90001 aaatatcggt gggaaggaca gcccaaacac cacgataacg ggaaacagac tcatgttgac
    90061 cttggttgta tagagagagc aggcgttatt attttcagca tctgtcgccg cagagaaggg
    90121 catggaaagc cgggcgagag caacattgct gtagattgat atttaatata ttagcgtaac
    90181 tgttatgctg ttatctatat tatgtgatct aaatcacttt taagtcagag tgaataatgg
    90241 aacgactaaa acgcatgtcg gtgtttgcca aagtagttga atttggctct tttaccgccg
    90301 ccgccagaca gctacagatg agcgtttcgt ccatcagtca gacggtgtca aaactggaag
    90361 atgagttgca ggtaaagctg ttaaaccgta gcacacgcag cattggcctg accgaagccg
    90421 gtagaattta ctaccagggc tgccgtcgta tgcttcatga agtgcaggat gttcatgagc
    90481 aactgtatgc cttcaataac acccccatcg ggacgctacg cattggctgt tcttcaacta
    90541 tggcacaaaa tgttctcgcc gggctgacag ccaaaatgct gaaagaatac ccaggtttga
    90601 gcgtcaatct ggttaccgga attccagccc ccgacctgat tgccgacggt ctggatgtgg
    90661 tgatccgcgt cggcgcgttg caggattcca gcctgttttc ccgccgtctt ggcgcgatgc
    90721 caatggtggt gtgcgccgcg aaaagctatc tcacacaata cggcataccg gaaaaacccg
    90781 ccgatttaag tagccattca tggcttgaat acagcgtgcg gcccgacaat gaatttgaac
    90841 tgattgcacc ggaagggatc tcgactcgcc tgatcccgca agggagattt gtgactaatg
    90901 atccgatgac gctggtgcgc tggctaacgg cgggtgccgg gatcgcctac gtgccgctga
    90961 tgtgggtgat caacgagatc aatcgtgggg agctggagat cctgctgccg cgttatcagt
    91021 cagatccacg cccggtttat gcgttatata ccgaaaaaga taagctgccg ctgaaggtac
    91081 aggtcgtgat caactcgctg acggattatt ttgttgaggt cggtaaattg tttcaggaga
    91141 tgcacgggcg cgggaaagag aagtaattca tattgtactg ttacgttgta caaacctgtg
    91201 ccaacgggtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gttcgtgcac
    91261 gaggaaggat taattatcct tctgaaaata ctgaaattat tacgcagtac cgccaacagt
    91321 caggttatca actttcagcg ttggctggcc cacgccaacc ggcaaacttt gcccttcttt
    91381 accgcagaca cccacgccgt tatccagttt caggtcgttg ccaaccatcg aaatctgctg
    91441 catggtttcg ataccggaac caatcaacgt tgcgcctttc accggcttcg ttactttacc
    91501 gttttcaatc agatatgctt ctgaagtgga gaaaacgaat ttgccggagg tgatatccac
    91561 ctgaccgcca ccaaagttcg gtgcatagat accgtactca acggattcaa taatttcctg
    91621 cggggtcgat ttacccggca gcatataggt gttggtcata cgcggcatgg gcagatgggc
    91681 gtaggattca cggcgaccgt tgccagtcgg cgtcatcccc atcaaacgcg cgttgagttt
    91741 atcctgcatg tagcctttca gaatgccgtt ctcaatcagc acgttgtact ggcctggcgt
    91801 accttcgtca tcaatcgcca ccgaaccacg gcgatcgacc atcgtaccgt catcaaccac
    91861 ggtacacagt tctgaagcca ccagctcccc gacctgtcca ctaaatactg aagtgccacg
    91921 gcggttgaag tcgccttcca gaccgtgacc taccgcttca tgcaacagca cgcccggcca
    91981 acctgcgcca agtactaccg gcatggtgcc cgctggtgca gcaacggcag aaagattgac
    92041 cagcgccata cgtactgctt cttttgccca tgcatccgca cggacttcgc cgtcgagatc
    92101 ggcaaggaag aactcataac caaaacgacc gccgccgcca ctggcaccgc gttcgcgttt
    92161 gccatcttct tcgatcagaa cgctcacgga aagacgcacc agcggacgga catccgccgc
    92221 tagcgtgccg tcggtggccg caaccagaat taattcatag acaccactga ggctggcagt
    92281 cacttcctgt acgcgcttgt ccgcttcgcg ggcaacctta tcgacgcgac gcaggatatc
    92341 cagcttctct tcacggctca tgctttgcag cggatctacc gaggtataca acgggctatg
    92401 ctctaccgcg cccagcgtct gtactttgcc atcaccgcta tcacgggcga tggtgcgcgc
    92461 cgcttgcgca ctctgttcca gcgccagcag gctgatttgg tcagcgtaag caaatccggt
    92521 tttttcaccg ctgattgcac gcacaccaac gccctgatcg atgttgtaag aaccatcttt
    92581 aataatgcgg tcttctaaaa cccaggattc gtgatagctc gactgaaaat agagatcgcc
    92641 ataatcaagg cgacgttcgg ccagttgacc gaggatcacg aacaagtcct gatgtttcag
    92701 gccgttcgcc gctagcaatt gttcacttac caggttaaga ctcatcgttt ttgctactcg
    92761 ttagttactg cagtagagga ttttttacgg ctgccggatg cggcgtgaac gccctatcca
    92821 gcctacggtt atgttctggc tcgttggcct ttggcaacga ttatcctatg agattggggc
    92881 aattacgcgc cctcgtcaaa tcattgcgct ttttctttac gcggttggcg caacacttcg
    92941 ttgatttgcg gatcgtccag cggacccgaa atgtgatagc gcaaaatgga gactttgctc
    93001 cacagcggcc ccagcacttt actggcggca aacactgccg cgccaacaat ggggttaacc
    93061 gcaaaagccg cagccacgcc cactgtcgca gaaatctctg gtgcgacaac cgcttccata
    93121 ttcaggtcgc gacgtaccag atttaccgac cctttcatgg cgatatccgc ctccaggcca
    93181 tccaccagcg tgtcgtcggt gtgcataacg ccgtctttaa tccacgcggt gctgcgaatg
    93241 gagtcaaaat agaacccttc gccaaaagtg tctctgaaat caaaacgcag cttacgcatc
    93301 agggcatcta cgctcaataa gcgcagcaat tgcccggcat gtccggtatt gatttcggta
    93361 atttcgcctt tacccagttg agtatgaatg atgccattca acgtcgcctc atctggctgc
    93421 cacggtgctt tacgccagtg taaatcgtaa tccacattaa atgacgactg ccgtatgggc
    93481 gtcgtgacac caaaaaattc tgcggcggca tcaattttct gcccgcgcag ttttcctttc
    93541 agcgaggtac gttcattccc cggattgtta atccattcac catcggcagt gagccgcgaa
    93601 aaaccagtat caatcagtcc attggtcagc gttaatgtat tgccagaaat ggtgatatca
    93661 ctgtcaatgc gcccgaattt ttgcccccag aaccagcact ctgcgcatcg tatttgggcg
    93721 tccgaccagc cacggaagtt aatgcgttcc gttgtcggga aaggtgatga cggcgttgaa
    93781 tcaccacgag ttttcgccac gctcgggttg taataaagat atttgatatt cgccagccac
    93841 ggcgcgttat tacgcatcgc cagcgtggcg ttgatttcac gcccctgcgc ctcaacctgg
    93901 gtgccatttg ccgtcggttg cgaaacaata ctcaggttat tccattgctg atttcccagc
    93961 gacaacatag gcgtacgtaa cgttatgtgt tgtgggaaac tcgctgcacc accgacactc
    94021 tccgccgcac ctttctgaaa cagggccagc cactcggcac cattcatcgg cggcatattg
    94081 agttcaacac cactttgttc cggcaacggc gggagcgttt tactgtctgc cgcccaaata
    94141 gcacggtcga gcgtcagctt ttgaccgagc aaccagcggc tattgaaatg attatccgca
    94201 ccagcctgtc cggttaattc aaagctgttg agattgccat caaccttaac gtttaccggc
    94261 agtggttcac ccgcaggttt ggctaacggt gaaggtaagt gactgctcac attcttcaaa
    94321 tcgccgttta gctctacgtt atacgtcgcg ccagcatgat aaggcagaac aatgcccact
    94381 ttaccatccc acgccacgct gccactcaat gcttcgttca ccgctgcagg cagaacgccg
    94441 gttttcgccg gttgccagtt accgttgaga ttcaccgcga cctggtaggc ttttgcccct
    94501 tctttggtgg aaaaatccac gttcaacggc tgattaaacc agcttgctgt cagtggttca
    94561 ctttgcagat cgccattgat aaagctgaat ttaccgctca aatttttcag ggtgctgtcg
    94621 agtggtttga taaacagact gttattacgc agcgtcactt cacctttcgc ggttaccagc
    94681 tcgccgttca gcgggatatc aagatgtaag cgagcattca catcgccgtc gagctggagt
    94741 tcttgcaggg tcgcacccag agaatctttc agcggtgtct catcaaagta agggccaacg
    94801 gctttacccg gacctttaat gtcagcgtca atcagcagtt tttcttttga gtagtcaggg
    94861 atcactgcgg tgagattact cgcgcgcacg ccgcccagat taacgccatc ggttttcatc
    94921 cataaaccgt cgttaataaa gtccagttca ataccaaggt tagttaatgc aggccagtcc
    94981 ggctggaagg caaacttcgc gttgcgcagc ggcaccagca cttcaaactg accttcgttg
    95041 tgtttatagg ggaagagttg aggattgcca ccataaacca gcgtcgcgtt atccgcttca
    95101 ccgccctgaa tcgcgccact taagtaatca accaggtctt tacccatcaa gttttccggg
    95161 aaatagcgcc aggcttgtga accatcatcg gtactgatgc cagccagaat acccagccag
    95221 ggttcatcgt tagcaggttg caggtaacga aaaccgccgc gcgcatggac ggctttggct
    95281 ttaacgtcaa tattacgccc atccagctgg aaacctttgt cattgttcag ccagcttata
    95341 gttgcctggc cgtcggcgat ttccagtggc gcacggaata ccgtttcgta aggcatcttt
    95401 gcctgcttca tcgacgcggt aagcaaacca ttttcaacgc tgccggaaag cgtcccggag
    95461 aagtgttccg cacccggtaa taatttccat tgcttccagg ccagatcgct ccacgatgcc
    95521 tgaaaacggg tcttgtctgc cgcctgaagc gggatatcca gcgccagagt gttaatcttg
    95581 ccgctcggtt gtgtagagcg ccaaacatca cccagtgcag gtgaaagttt cgcggccagc
    95641 gggcgtatgc cctccaggcc tgccagctcc agattactgg cgcgaatccg gagttcgtca
    95701 ctgcgtttat tgtctttgcc gccaacgtcc tgttccggta tccaggccag cgtcaatgct
    95761 ccgctcggcc agggtttgcc gtccatcgtg atccgtgtat cgggaataga gaactgccat
    95821 cccggatttt cacgcgtaat atgcgcggtc agattatcca ccgacagcgt atgcgtttcc
    95881 ttctcgccca accagctggc accaccctgt ttcagccaga cgtcaccgcc ggttacatcg
    95941 cctttgtcga tcgtcatcca gccttcaagg gagaactgcg ccgtttccag cgcaatattg
    96001 tcctgcatcc atttaccgag ccacggcttc aggtcgatgt catccgcctg gagccagacg
    96061 cgaccattgc ttaacaaccc ctcatcatcg cgcaaatcca tgcgcacctg catcacgccg
    96121 tgctgtccgg taaggctgga gaggcttacc aggccttccg cacggtgtcg acgtggatcg
    96181 ttcagccagg tgagttgtgg gatcgccagc tcggcgcgct gaccggatgg cgtcaggaaa
    96241 ctgacttcac tgtcgcggag atcgaaatgg tcaaattgac gaagaaacag atcgctgatg
    96301 tgactggctt ccagactgtc atcgctacca ccgctggtga taggagtgtt ggtgcgaaag
    96361 cgcagctgcc agaaagtgag gtcgcgaaac tgccagcgca tatgtaacag gctctgccag
    96421 acatccagcg ccagagtaac gcgtttaacc gaaaattcgc cgccatcctt tagttctgca
    96481 cggatgtcgt gcgcttcaag cgtcgggcca aaattctgcc agctggctga gagctgactg
    96541 gcttctaccg gcatgccagt cgcggattct attttgttga ggatttccgg acgccaggcg
    96601 tcaagatgcg gtaaagcaat acgcaggccg ctaaccagca gggcagcgat cacaacgagc
    96661 gcggctccag taagcagtaa aatccccggc aatcgcctca cccgtcactc cttgtctgct
    96721 aaaaatgtga ctcaaaaacc ctttgccgga tggcggccca gcatctgttt acatcattac
    96781 gacgtcaaac tgctcctggt tatagagcgg ttcaatttgt actttaacct gtttgccaac
    96841 gaaaatttcc acttccgcca gcgagtgtga ctcttcgcct ttcaaggctt cggctactgc
    96901 cggagaagca tagaccagga aacggtcgga gtcgtaagca tggtgtacac gaacaatctc
    96961 gcgcatgatt tcatagcata ccgtttccac ggttttcacc gttccgcgac cgtggcaggt
    97021 tgggcattcg ttacacagta cgtgctcaat gctttcgcgg gtgcgtttac gcgtcatctc
    97081 caccagcccc agcgccgaaa aaccattaac gctggttttc acccggtctt tgctcaacgc
    97141 ctgctccagc gagtgcagca ctcggcggcg gtgatcttca ttattcatat cgatgaaatc
    97201 aataatgata atcccgccca gattacgcaa ccgtaactgg cgagcgatag cctgcgtcgc
    97261 ttcaatattg gtattgaaaa tggtgtcgtc cagattgcga tgaccgacaa acgctccggt
    97321 attgatgtcc acggtggtca tcgcttcggt ctggtcgata atcagataac caccggattt
    97381 cagttcaact ttgcgttcca gcgctcgctg gatttcgttt tcgacatcaa agagatcgaa
    97441 aatcggctgg cgtccggtgt aatgctccag cttgctggtc atctcgggaa tgtactccga
    97501 ggtgaactca agtaacgctt cgtaagtcag gcgtgagtca acgcgaatgc ggtccagttc
    97561 ggcatcggcg aaatcacgca gaacacgctg cgccaacgcc agttcgccgt acagctgata
    97621 acgggtctgc gggcgctttt tacgctccat aactttggtc cagacgcgtt tcagataagc
    97681 ggcatcggag gccagttcag cctcgccaac cccttccgct gcggtacgga tgataaaccc
    97741 gccctgctcg tcgcaatact ctgcgaccac ttttttcagg cgttcacgtt ctgattcgct
    97801 ttcaattcgt tgggaaaccc caacgtgaga agcccctggc ataaagacca gatagcgaga
    97861 aggcagcgtg atatcggtgg tcaggcgcgc ccccttagtg ccaagcggat ctttcaccac
    97921 ctgcaccatc agatcttgcc cctgacgaac cagttccgag atgtcgcgca ccgtgaattg
    97981 cttttgttct tcacccgcca cacattcggt gtgcggcatg atgtcagatg catgaagaaa
    98041 cgcggcttta tccagcccaa tatctacaaa agccgcctgc atacccggaa gtacacgact
    98101 tacacgaccc ttgtagatat tgcctactat tccgcgtcgc gcctcacgtt caatatgaat
    98161 ttcctgcaga ataccgccat caatatacgc cactcgcgtt tccgaaggcg ttacgtttac
    98221 taacaattca gccgtcatgt ttatcccttt tctcacgcag tgcgttaaaa ttacttaata
    98281 attcatacgt ttcaaccagc ggtaagccga ctacggcttg atagctgcca tttatcttcc
    98341 tgacaaaaca gccacccagc ccctgaatac cgtatgcacc tgctttatct aacggttcac
    98401 cgctggcgac atagcccgcg atgtcttcgt ctgttaacgt tctgaaagtc acatcggtga
    98461 ccaccaggca atcgagaatg tgccggctgt cggccaacgc cactgccgtc atcacctgat
    98521 gggtctgacc cgataatttg cgcaacatct gcgccgcatg ctctgcgtcg cgcggtttct
    98581 ccagcacttc tccgttcagg ataacgatag tatccgcacc cagcaccggg agatccttcg
    98641 ccgtttgcgc gacacctgcc cgtgctttct cgcgcgccag acgcacaaca tactgctgcg
    98701 cgctctcctg cggctgacgc tgctcctcaa tgcccgtaac aatacgttca aaggtcacgc
    98761 caagttgcgc aagtaactcc tgacgacgcg gagaaccgga agctaaatac agagaagtca
    98821 tagaaacctt tattgcactg caaactgctg acggacttta cgcatcagca agaaaatcca
    98881 cggccagagc accccattga ctacactact ccagaacact tccggtctga aagagacgtt
    98941 aatcactaaa aactctgccc agaaaacaat aatatccacc accagcgaaa gcaacatgac
    99001 gaccagcgcc tgctgccata atgcgaggtt gcggaaaagc tggtatttca gcgccaccag
    99061 gtaagcaatg atgctcatcg ccaatacgcg tacgccaagc gtcgagccgc tgatcagatc
    99121 cagtatggca cccatcacaa aacctgtgcc cacatttacg cgatggggca aggccaggat
    99181 ccaatacaac aagatgagta acacccagtt tggccggaaa acaatcaggt tatccggcca
    99241 gggcatgatt tgcagcaaaa gcgcaatgag gaaagagagc cagattaccc agcgtccctg
    99301 gctacgatag ctcgccacta ttgccctccc ggcgcacgcg caggcggttg agcaccactt
    99361 tgcggcggcg tagccctttg tagagagcga ttagcagcgg gctgtgtggg cgcagcaggc
    99421 gcagtagctg catttcctgt cgtgggttgc tgcggagtcg gctgagcgat ccccgttgcc
    99481 ggttcaggta actttggccc catcgcgtct ggcgaaggca atacctgcgg catcatctgc
    99541 atcagacgtt cattagcaac acgatgcacc tcttccggcg tcatcgggtt agcgccgtta
    99601 cgatctgccc cccacagcag cagcagataa cgcaaacgtt gcagccctgc agtcggacgc
    99661 gcctgaatca cagtataagc gcgctgggta tcgagtttta cggaagagac aaccgcgacc
    99721 ggatagcctt ccgggaaacg accgcccaga ccggaggtca ccagcacatc accaacacga
    99781 atatccgtat tcgctggcag atgctcaagc tgcaaatcat ccgtacaacc gttaccggct
    99841 gcaattacgc ggatatcgtt gcgcagcacc tggattggca gcgcgtgggt cgcatcacaa
    99901 atcagcagca cgcgactggt cagtttagcg acggccacca cctgaccaac aacacctttg
    99961 tcgctgatga ccggctggcc ttcataaacg ccattaacgc tacctttatc gataacaact
   100021 tgatcgctat aaggatcgtt aaccgtggag ataacctgag tcaccatttt ctgctcatcc
   100081 tgacgcagcg gggaacccag cagctcgcgc agacgcgcgt tctcctgttt gtattgtcca
   100141 agcatcagca gttcactgtt tttcagcaac agttcctgac gtaacgcccg attttcaagt
   100201 tctagttggt cacgcgaggc cagcgtctgc gatacgccat ccagcaattc acgaggagca
   100261 ttggaaacaa agtagaaagg actgacggcg gtatccatat aagtacggat ttgactgaac
   100321 gtccccaggc ggctgtcggc aataataatg ccgagcgcca ccagcaccgc cagaataagg
   100381 cgaatctgta gcgacgggcc acggctaaaa attggcttca taagttatgc gtattctcgt
   100441 atcagaccag gcagggtaaa cagacgcttc ccctgcctgc atccgattac tcttcgctga
   100501 acaggtcgcc gccgtgcatg tcgatcattt ccagcgcttt gccgccaccg cgcgccacac
   100561 aggtcagcgg gtcttcagca acaacgactg gaatgccggt ttcttccatt aacaaacggt
   100621 caaggttacg cagcagtgcg ccaccaccgg tgagtaccat gccgcgctcg gagatgtcgg
   100681 aagccagttc cggcgggcac tgttccagtg caaccattac cgcgctcaca ataccggtca
   100741 gcggttcctg cagtgcttcg aggatttcgt tggagttcag ggtaaaaccg cgtggaacac
   100801 cttctgccag gttacggcca cgaacttcga tttcacggac ttcatcgccc ggataagccg
   100861 aaccgatttc gtgcttgata cgttctgcgg tggcttcacc gatcagagaa ccgtaattac
   100921 gacgcacata gttgatgata gcttcgtcga aacggtcacc accaatgcgt acggaagagg
   100981 agtaaaccac gccgttcaag gagataacag caacttcagt ggtaccacca ccgatatcaa
   101041 ccaccataga accggtcgct tcagaaaccg gcaggccagc accaattgcg gcagccatcg
   101101 gttcttcaat caggaagact tcacgggcac cagcgccctg cgcggattca cgaattgcgc
   101161 ggcgttcaac ctgggtcgcg ccaaccggta cacaaaccag aacgcgcggg cttggacgca
   101221 taaagctgtt gctgtgcact tgtttgatga agtgctggag cattttttca gtcacgaaga
   101281 agtcggcgat aacgccgtct ttcattgggc gaatggcagc aatattgccc ggcgtacggc
   101341 ccagcatctg cttcgcgtca tgacctactg cagctacgct tttcggtgaa ccggcacgat
   101401 cctgacgaat ggccaccacg gaaggctcat tcaatacgat gccttgtcct tttacataaa
   101461 tgagggtatt cgcagtaccc aggtcaatgg acaagtcatt ggaaaacatg ccacgaaatt
   101521 ttttcaacat actaagggat aatcctgaaa gctggggcgg aaaagaaaat ccgcttactt
   101581 taccaaccac acgcagcagc gacaaggcgc aaaaatcatc tgctacggtg aaaattagtg
   101641 cagttcgttt cctttgttac aaatctctgc ctgagtccag aaaggcttaa tgcatcagca
   101701 gcattcctcg cctgtttgca accgcgtaag gtcattcatc tgcatatgtg ctgcaacaat
   101761 ctggcgagca gacaagcaca ctcccatgag acgcagcgcg cgttattcta cgtgaaaacg
   101821 gattaaacgg caggttaaac cgagtatctt tgtgaatatt ttttcacgtt agtatcaagt
   101881 ggctgtgagg acgcgaaaaa atccccttgc ccgcctgtaa caccgcgctg aatcagggtc
   101941 tgccactcgc ttcgcgaacg cacaccggtg gcgtaaacct gggtgctggt ccctgagcag
   102001 gcttccacca ggctttgaac cagcagctgg ttctccgttc gcttctcaat gtttctgacc
   102061 agccccggat ggagcttgag taactcaaca ttaagttctt tgatccaact ggtgcttacc
   102121 agcgtcaaac cagcctggtt gacggctacc cgtaccccta aagcattcac taaacgaata
   102181 acaggttgta aacgactgat atgttgacct acatcggcct ctgcaagttc aataattatg
   102241 cgttttcgtt gtgatttttc acattgcatt aacgtatcgc gcagccaacg ctgaaaacgc
   102301 gggcgaatca gcgactcaac ggtaacctga atcgccagat tttcctctgg ccagtaacgc
   102361 aatagtggaa taagacggct gatttgcaga cggtcatact cttccgataa gccaaactgc
   102421 aagaccatcg gcatatactc cgccgagcta acctcttcat taccatcaaa gatgcggcac
   102481 atgagttcgc gatgatgaac ctgaccttcg cgagtaaccg ccggtttttg ataaaggcgc
   102541 gggccgccgc gactgagcat ttgctcgata agcgtacgcc agcgaacatt accgcgtcct
   102601 ttttcaggca acgagtcatc gtaaatagcc cagctattgc cgccctgcaa tcccgcatta
   102661 cgcgtggcag actctgcatg ttccattacc tgctcggtat cctgaccact acgccaggcg
   102721 cagataccaa tgtggatcat atcgtcgcga tcgagcattt tattgttcgg caaggtatcg
   102781 acggctttga ttaactgacc ggcgatgctc tctgcctctt ttaacgtccg gtgcggtaac
   102841 agcgcagcaa aatcactgcg gtggtaacgc gccagcagtg cgccagggta gcgcatcata
   102901 aatgtcgaca gcagattcgt cagagtgaag aactgttctt caacctggct gtgcccccag
   102961 gtatcgctca acatattgaa atccggcaga cgaatcatca tcacgatccc gtgggtacct
   103021 actttctcct gatcttccag taacgttgct aactgattat cgaaaaagag tcggttattg
   103081 aggccggttt tcacgtcctg tgcggcataa gagcggatca gcgtatcaag acggctgtgt
   103141 tgttcgcgtg cgttctgaat ttcacgaagc agcgtatcca gcgcactgct ggttctgggc
   103201 ggccattcat agatggttcc caacacatta gagccacgct caccgtttaa gatacgagta
   103261 gcccgggttt ccagcaattc ttgcccggca agttgccgtt gtaaccagcg taccgccaga
   103321 aagagcataa gaatgataaa gccaatcgcc cctgtgagcg gcgcggtggt catcaacgaa
   103381 tggaaatagt tgcccatcgg atcctgataa accagacgca acgacatccc cggatgcttt
   103441 atcaacggaa cgctcagttc acgaaacaga tcgctggtgc caaccggacg ataactacca
   103501 tttctggcca gggtataaac ctgcttatca ccatggagca ggtctacacg aacgatatct
   103561 gccgacatca ttaattcggt aatttgtggc cttaatgcgc tgaagtcatt cgacacaagg
   103621 tgggtatcaa tcgccgtcgc caccgcctga acgcgatgac taaacttata ctgaatggcg
   103681 ttgtagaaac ttagcgaaca gcccagcaaa gtcacaaaaa ttgttaaccc ggtgagcagc
   103741 gtaacaaagg ccgaaaattt cgtcgttaat ctcatccttg tgttaactcc gatagtgagg
   103801 aagcgggcat actagcaaat cagatttatc tcgcaattta ttgcgcttca tcggctttgc
   103861 ttttccatta gcgagtatag tcttcagaaa ttattttcca atccatcatg cacatgagga
   103921 ccacttatgc aggcgttact tttagaacag caggacggca aaactctcgc atcagtacag
   103981 actctggacg aaagtcgcct gccggagggc gatgtcacgg tcgatgttca ctggtcgagc
   104041 ctgaactata aagatgcgct ggcgattacc ggtaagggaa aaatcatccg taattttccg
   104101 atgattcctg ggatcgattt tgccggaact gtacgcacca gcgaagatcc gcgttttcat
   104161 gccggtcagg aggtgttact cactggctgg ggcgttggtg aaaaccactg gggtgggctg
   104221 gcggagcagg cgcgagtgaa aggtgactgg ctggttgcca tgccgcaagg gctggacgcg
   104281 cgtaaagcaa tgattatcgg tactgccggt tttaccgcca tgctgtgtgt gatggcgctg
   104341 gaagatgccg gtgttcgccc gcaggacggg gagattgtcg tgacgggtgc cagtggtggc
   104401 gtcggcagta ccgccgtggc gctgctgcat aagttgggtt atcaggtcgt tgccgtttcc
   104461 ggtcgcgaaa gtacccatga atatctgaaa agtttaggtg ccagccgtat tctccctcgt
   104521 gatgagtttg ccgaatcccg tcctctggaa aaacaagtct gggctggggc aattgacact
   104581 gttggcgaca aagtgctggc aaaagtgctg gcgcaaatga attacggcgg ctgcgtggcg
   104641 gcctgtggtc tggcgggtgg ttttactctg ccaaccacgg tcatgccatt tattctgcgt
   104701 aatgtccgtt tgcaaggggt ggattcagta atgacgccac cagaacgccg cgcacaagcc
   104761 tggcagcgac tggtcgccga tttaccggaa tcattctata cccaggcggc aaaagagata
   104821 tctctgtcag aggcaccgaa ctttgccgag gccatcatta ataaccagat ccagggtcgc
   104881 acgctggtga aggttaacta accatttagc aaggaataat aagagaggga actcattttg
   104941 aaatcattat tcaatcggtt aacgggaaaa gcggttagcc ggacagcttt cgtcgaacac
   105001 cttggtcatg aagttgtaca acatcatcca aactggaaag tcatgatttc gactgaccat
   105061 aaattgatgc gcattgatac tccactaaac agctattatt gatacgcctc cgtcgcctgt
   105121 taggtttatg gtgctttgcc tgggcgacgc tacgcttaac cccttactta tttctggtac
   105181 catgaggtga ataatctgat tttgtttgac tacaaattaa tcactcgaac ctatttaatg
   105241 ctgagcattg tcaatcggtt aattttgcgt gctttagcat tcacatctat ccaggcgatg
   105301 cagtgaaaat tgggtaatcc ccagcaaccg ctgcgtaatg tcgtctatct tgtcgcgatc
   105361 ctggcatccc tacattattt gtggtctgtg acgattatct cattgcagcc cctcatcttc
   105421 gcagggctgg ctttacagct tttcacctta cgttataaga agttccgtcg atgatggcgc
   105481 taatttcgtg aattgtgcgg cttgttgcaa attacacggt gttgaaggtt atttacatgt
   105541 tagttgttga ttatcttccc tgataagacc agtatttagc tgccaattgc tacgaaatcg
   105601 ttataatgtg cgacctcgtc ctccctgacg cagtttttgc gctgcggaaa aggtgacatt
   105661 ggcgcaacga aggtatattt tgttttttgc cggaggatag cagcagatcg ctgcacaatg
   105721 tccgtcaagt ctaacattga cactctgggg caaaatagac cggcgtcccg acctgctgga
   105781 atttatcgct atgcatacag ctgtcggggc atacgcttta cagacggcgg tgaaacgcct
   105841 gtcacaatca cactaaacaa agagtacgga acccactcat ggatattcgt aagattaaaa
   105901 aactgatcga gctggttgaa gaatcaggca tctccgaact ggaaatttct gaaggcgaag
   105961 agtcagtacg cattagccgt gcagctcctg ccgcaagttt ccctgtgatg caacaagctt
   106021 acgctgcacc aatgatgcag cagccagctc aatctaacgc agccgctccg gcgaccgttc
   106081 cttccatgga agcgccagca gcagcggaaa tcagtggtca catcgtacgt tccccgatgg
   106141 ttggtacttt ctaccgcacc ccaagcccgg atgcaaaagc gttcatcgaa gtgggtcaga
   106201 aagtcaacgt gggcgatacc ctgtgcatcg ttgaagccat gaaaatgatg aaccagatcg
   106261 aagcggacaa atccggtacc gtgaaagcaa ttctggtcga aagtggacaa ccggtagaat
   106321 ttgacgagcc gctggtcgtc atcgagtaac gaggcgaaca tgctggataa aattgttatt
   106381 gccaaccgcg gcgagattgc attgcgtatt cttcgtgcct gtaaagaact gggcatcaag
   106441 actgtcgctg tgcactccag cgcggatcgc gatctaaaac acgtattact ggcagatgaa
   106501 acggtctgta ttggccctgc tccgtcagta aaaagttatc tgaacatccc ggcaatcatc
   106561 agcgccgctg aaatcaccgg cgcagtagca atccatccgg gttacggctt cctctccgag
   106621 aacgccaact ttgccgagca ggttgaacgc tccggcttta tcttcattgg cccgaaagca
   106681 gaaaccattc gcctgatggg cgacaaagta tccgcaatcg cggcgatgaa aaaagcgggc
   106741 gtcccttgcg taccgggttc tgacggcccg ctgggcgacg atatggataa aaaccgtgcc
   106801 attgctaaac gcattggtta tccggtgatt atcaaagcct ccggcggcgg cggtggtcgc
   106861 ggtatgcgcg tagtgcgcgg cgacgctgaa ctggcacaat ccatctccat gacccgtgcg
   106921 gaagcgaaag ctgctttcag caacgatatg gtttacatgg agaaatacct ggaaaatcct
   106981 cgccacgtcg agattcaggt actggctgac ggtcagggca actctatcta tctggcggaa
   107041 cgtgactgct ccatgcagcg ccgccaccag aaagtggtcg aagaagcacc agcaccgggc
   107101 attaccccgg aactgcgtcg ctacatcggc gaacgttgcg ctaaagcgtg tgttgatatc
   107161 ggctatcgcg gtgcaggtac tttcgagttc ctgttcgaaa acggcgagtt ctatttcatc
   107221 gaaatgaaca cccgtattca ggtagaacac ccggttacag aaatgatcac cggcgttgac
   107281 ctgatcaaag aacagctgcg tatcgctgcc ggtcaaccgc tgtcgatcaa gcaagaagaa
   107341 gttcacgttc gcggccatgc ggtggaatgt cgtatcaacg ccgaagatcc gaacaccttc
   107401 ctgccaagtc cgggcaaaat cacccgtttc cacgcacctg gcggttttgg cgtacgttgg
   107461 gagtctcata tctacgcggg ctacaccgta ccgccgtact atgactcaat gatcggtaag
   107521 ctgatttgct acggtgaaaa ccgtgacgtg gcgattgccc gcatgaagaa tgcgctgcag
   107581 gagctgatca tcgacggtat caaaaccaac gttgatctgc agatccgcat catgaatgac
   107641 gagaacttcc agcatggtgg cactaacatc cactatctgg agaaaaaact cggtcttcag
   107701 gaaaaataag actgcaaaag cgtcaaaagg ccggattttc cggccttttt tattactggg
   107761 gatcgacaac acccataagg tacaatcctc gctttcttca cccatcaggg acaaaaaatg
   107821 gacactcgtt ttgttcaggc ccataaagag gcgcgctggg cgctggggct gacccttttg
   107881 tatctggcag tttggttagt agccgcttac ttacctggcg ttgcccccgg ttttaccggc
   107941 tttccgcgct ggtttgagat ggcctgcatc ctgacgccgc tgctgtttat tggactgtgc
   108001 tgggcgatgg tgaaatttat ctatcgcgat atcccgctgg aggatgacga tgcagcttga
   108061 agtaattcta ccgctggtcg cctatctggt ggtggtgttc ggtatctcgg tttatgcgat
   108121 gcgtaaacgg agcaccggca ccttccttaa tgagtatttc ctcggcagcc gctctatggg
   108181 cggtattgtg ctggcgatga cgctcaccgc gacttatatc agtgccagtt cgtttatcgg
   108241 cgggcctgga gctgcttaca aatacggcct tggctgggta ttgctggcga tgattcagct
   108301 tccggcagtc tggctttcac tcggtattct cggcaagaag tttgcgattc ttgcgcgccg
   108361 ctacaatgca gtgacgctga acgatatgct gtttgcccgc taccagagtc gtcttctggt
   108421 gtggctggcg agtttgagtt tgctggttgc gttcgttggt gcgatgaccg tgcagtttat
   108481 cggcggtgcg cgcctgctgg aaaccgcggc gggtattcct tatgaaaccg ggctgctgat
   108541 ttttggtatc agcattgcgt tatataccgc ctttggtggc tttcgcgcca gcgtgctgaa
   108601 cgacaccatg caagggcttg tgatgctgat tggcaccgtt gtgctgctta ttggcgtggt
   108661 acatgccgct ggcggcttaa gcaacgcagt acagaccttg caaaccatcg atccgcaact
   108721 ggttacgcca caaggcgctg acgatattct gtcgcctgcc tttatgacgt cgttctgggt
   108781 actggtgtgc tttggcgtta taggcctgcc gcatactgcg gtgcgctgta tctcttataa
   108841 agacagcaaa gcagtacacc gggggatcat catcggtacg attgtagtcg caatcctgat
   108901 gttcggtatg cacctagccg gagcgttagg tcgtgcagtg atccccgatc tcaccgtacc
   108961 ggatctggtg atcccgacgt taatggtaaa agtgctgcca ccgtttgctg ccgggatctt
   109021 tctggctgca cctatggctg cgatcatgtc gacaattaac gcccaattgc tacaaagttc
   109081 cgctacgatc attaaagatc tctatctgaa tatccgtccg gatcaaatgc aaaacgagac
   109141 gcgtctgaag cggatgtcgg cggtaattac gttagttctc ggcgcgttgc tgctgcttgc
   109201 cgcctggaag ccgccagaaa tgatcatctg gctgaatttg ctggccttcg gtgggctgga
   109261 agccgttttc ctgtggccgc tggtgctggg tctttactgg gaacgcgcca acgccaaagg
   109321 cgcgctaagt gcgatgatcg ttggcggcgt gctgtatgcc gtactcgcga cgctgaatat
   109381 tcagtacctg ggcttccacc ctatcgtgcc ctcgttacta ctaagtttgc tggctttcct
   109441 ggtcggaaac cgtttcggta catccgtccc gcaagctacc gttttgacta ctgataaata
   109501 aagagttttg ccatgccttg gatccaactg aaactgaaca ccaccggcgc gaacgcggaa
   109561 gatcttagcg atgcgctgat ggaagcgggt gccgtttcta tcacttttca ggatacccac
   109621 gatacgccag tatttgagcc gctgccgggc gaaacgcgcc tgtggggcga caccgatgtg
   109681 attggtctgt tcgacgctga aaccgatatg aacgacgtgg tggcgattct ggaaaaccat
   109741 ccgctgctcg gcgcaggctt cgcgcataaa atcgaacaac tagaagataa agactgggag
   109801 cgcgaatgga tggataattt ccacccgatg cgctttggcg aacgactgtg gatctgccct
   109861 agctggcgtg atgtgccgga cgaaaacgcc gtcaacgtga tgttagatcc agggctggcg
   109921 tttggtacgg gtacccatcc aaccacctct ctgtgcctgc aatggctcga cagcctcgat
   109981 ttaaccggta aaacagtcat cgactttggc tgtggttccg gcattctggc gatcgcggcg
   110041 ctgaaactgg gtgcagcaaa agccattggt attgatatcg atccgcaggc gattcaggcc
   110101 agccgcgata acgccgaacg taatggcgtt tctgaccgtc tggaactcta cttaccgaaa
   110161 gatcagccag aagaaatgaa agccgacgtg gtggtcgcta acatccttgc aggcccatta
   110221 cgtgaactgg caccgttaat tagcgtcctg ccagtttcag gcggcttact gggcctttct
   110281 ggtattctgg caagccaggc ggagagcgtt tgcgaagcct atgccgacag cttcgctctg
   110341 gacccggtcg tggaaaaaga agagtggtgc cgtattaccg gtcgtaagaa ttaaccttcg
   110401 catcgccata gggtgacgcg gggcgaagtg cgagcaagct cacaaaaggc acgtaaattt
   110461 gccgattatt tacgcaaatt tgcgtgccaa aattttcatt cataaagaaa aattgagaac
   110521 ttactcaaat ttctttgagt gtaaatttta gtcactattt tctaatatga tgatttttat
   110581 gagtaattat cgcaccacgc tcattttaaa tgcaattctt tgatccatct cagaggattg
   110641 gtcaaagttt ggcctttcat ctcgtgcaaa aaatgcgtaa tatacgccgc cttgcagtca
   110701 cagtatggtc atttcttaac tcatgcgcat cggacaatat cagctcagaa atcgcctgat
   110761 cgcagcgccc atggctggca ttacagacag accttttcgg acgttgtgct acgagatggg
   110821 agccggattg acagtatccg agatgatgtc ttctaaccca caggtttggg aaagcgacaa
   110881 atctcgttta cggatggtgc acattgatga acccggtatt cgcaccgtgc aaattgctgg
   110941 tagcgatccg aaagaaatgg cagatgcagc acgtattaac gtggaaagcg gtgcccagat
   111001 tattgatatc aatatgggtt gcccggctaa aaaagtgaat cgcaagctcg caggttcagc
   111061 cctcttgcag tacccggatg tcgttaaatc gatccttacc gaggtcgtca atgcagtgga
   111121 cgttcctgtt accctgaaga ttcgcaccgg ctgggcaccg gaacaccgta actgcgaaga
   111181 gattgcccaa ctggctgaag actgtggcat tcaggctctg accattcatg gccgtacacg
   111241 cgcctgtttg ttcaatggag aagctgagta cgacagtatt cgggcagtta agcagaaagt
   111301 ttccattccg gttatcgcga atggcgacat tactgacccg cttaaagcca gagctgtgct
   111361 cgactataca ggggcggatg ccctgatgat aggccgcgca gctcagggaa gaccctggat
   111421 ctttcgggaa atccagcatt atctggacac tggggagttg ctgcccccgc tgcctttggc
   111481 agaggttaag cgcttgcttt gcgcgcacgt tcgggaactg catgactttt atggtccggc
   111541 aaaagggtac cgaattgcac gtaaacacgt ttcctggtat ctccaggaac acgctccaaa
   111601 tgaccagttt cggcgcacat tcaacgccat tgaggatgcc agcgaacagc tggaggcgtt
   111661 ggaggcatac ttcgaaaatt ttgcgtaaac agaaataaag agctgacaga actatgttcg
   111721 aacaacgcgt aaattctgac gtactgaccg tttctaccgt taactctcag gatcaggtaa
   111781 cccaaaaacc cctgcgtgac tcggttaaac aggcactgaa gaactatttt gctcaactga
   111841 atggtcagga tgtgaatgac ctctatgagc tggtactggc tgaagtagaa cagcccctgt
   111901 tggacatggt gatgcaatac acccgtggta accagacccg tgctgcgctg atgatgggca
   111961 tcaaccgtgg tacgctgcgt aaaaaattga aaaaatacgg catgaactaa ttcaggttag
   112021 ctaattgctt gattaaaagg cgctactcgg catggggaag cgcctttttt ataggtgtca
   112081 caaagggagt gaccatgaga acaggatgtg aaccgacccg gtttggtaat gaagctaaga
   112141 ccattattca cggtgatgcc cttgccgaac ttaaaaagct acctactgaa agcgtcgatc
   112201 tgatctttgc cgacccaccg tataacatcg gtaaaaattt tgatggtctg atcgaagcct
   112261 ggaaagaaga tctgtttatc gactggctgt tagaagtgat tgcagagtgc caccgcgttc
   112321 tgaaaaagca gggcagcatg tacatcatga acagtacgga aaacatgccc tttatcgatc
   112381 tccagtgccg caagcttttt accatcaaaa gtcgcatcgt ctggtcatat gacagttctg
   112441 gagtgcaggc gaaaaaacac tacggctcca tgtacgaacc catcctgatg atggtgaaag
   112501 acgcaaagaa ctacacattc aacggtgatg ctattctggt agaagccaaa accggatcgc
   112561 agcgcgcgtt gatcgattat cgcaaaaatc ctccacagcc ttacaatcat caaaaagtac
   112621 cgggtaacgt ctgggatttt ccgcgcgtgc gttatttgat ggatgaatat gaaaaccacc
   112681 cgacgcaaaa accggaagcc ttactgaaac gcattattct cgcctcttcc aacccaggcg
   112741 atatcgttct cgacccgttt gctggtagct ttactaccgg tgccgtagcc atcgccagcg
   112801 gacgaaaatt cattggtatt gagatcaaca gcgagtacat caaaatgggg cttcgacggc
   112861 tggatgtcgc gtcgcattac tctgcggaag aactggcgaa agtgaaaaaa agaaagaccg
   112921 gcaacctgtc aaaacgaagc cggttaagcg aagttgaccc cgatctcatt acaaagtaaa
   112981 ggatgtgtaa gcctggtttg cagattattc atttcgtgta tatttcctgc cagacttggt
   113041 taaacatgca caggctcagg taatgattcg caagtattgg tggctcgtcg ttttcgctgt
   113101 cttcgttttt ctgtttgata ctttactgat gcagtggatt gaactgctgg caacagaaac
   113161 agacaaatgc cgcaatatga actcagttaa tccactaaaa ctggttaact gtgacgaact
   113221 gaattttcag gacagaatgt gaatttacat gacacttaat tcattcgttt gatgaattaa
   113281 tttcgttatg ttttcatctg gcatgaacat tcttaatacg ttatcgacca gagcgggggc
   113341 ttgtttataa agatcataac ccgccatatt cattaaccag ttttgaacaa ttccgctgaa
   113401 ggcaccatca ataataatca tcacaacatc taaatcgagg ttatttgcta cacaaccttg
   113461 ttgctgacac gcctgcaata cttcgcggag agtctgcgga ttaaagccca tcttttcgcg
   113521 tatcactccc tcggccagca tctcatcatt aaattcacat ttgtgatata agattttcag
   113581 caacgcctgc tggcggggaa ttttggcaat atattgcaag ccgacaatca atttttcacg
   113641 caattgttga aacgggtcat gctctaatcc agccgtcaag tgttcctgga ttaactcccg
   113701 caatgaaggc tgttgcaacc acatctcatt aaacagttgg gtcttgttct cgaagtgcca
   113761 gtagatagcg ccacgcgtaa cgttagcggc atcggcaatg tcgttgagcg tcgtcttgct
   113821 tacgccatgt tgcgcaaact gggcgatggc agtttcaatc agttcttgcc gggtcttcag
   113881 agcttcggct ttggttcttt ttgccatgat taattattca ggaaataaat atcttcgaca
   113941 cagagtgaga aaatagcgaa ggttaatcta tcacctaatg tgtatttata cgagaggcta
   114001 acattgagtt gctataaatc gttaaataaa taatatatat tatttactta agatacattc
   114061 actacatcaa tatatacttc aatttacgat tttttaattc tgcctctttc aacccgcgtc
   114121 aaaataaaac agtagaatat taatcttttt ttgtgtttat gtgccttgag acgcctgtat
   114181 tcataactat tccttacatc gacgaatgat aatttgtagg atagcgaact gtatttttct
   114241 ttctgcgagt taacgcgttg cctttttggg taaataacgc gcttttggtt ttttgaggaa
   114301 tagtaatgac gaaacatgcc aggtttttcc tcctgccctc ctttattctg atctccgcgg
   114361 ctttaatcgc cggttgtaac gataagggag aagagaaagc tcacgtcggt gaaccgcagg
   114421 ttaccgttca tattgtaaaa acggccccgt tagaagttaa gactgaatta ccaggccgca
   114481 ccaatgctta tcgtatagcc gaagttcgcc cacaggttag cgggatcgta ctgaatcgca
   114541 atttcactga aggcagcgat gtgcaagcag gccagtccct gtaccagatc gatcccgcga
   114601 cctatcaggc aaattatgac agcgcgaaag gcgaactggc gaaaagtgaa gccgccgccg
   114661 ccatcgcgca tttgacggta aaacgttacg ttccgctcgt gggtacgaaa tacatcagcc
   114721 agcaggagta cgaccaggcc attgctgatg ctcgccaggc cgatgccgcc gtgattgccg
   114781 caaaagccac agtcgaaagc gctcgcatca atcttgctta taccaaagtg actgcgccaa
   114841 ttagcggacg tatcggcaaa tcgactgtga ccgaaggcgc tcttgtcact aatgggcaaa
   114901 cgactgaact ggcaactgtc cagcagctcg atcctatcta tgttgatgtg acccaatcca
   114961 gcaacgattt tatgaggctg aagcagtccg tagagcaagg aaatttgcat aaggaaaacg
   115021 ccaccagcaa cgtagagttg gtcatggaaa acggtcaaac ctatcccctg aaaggtacgc
   115081 tgcaattctc cgatgtgacc gttgatgaaa gcaccggctc cataacccta cgtgctgtct
   115141 tccctaaccc gcaacatacg cttttgccgg gtatgtttgt gcgtgcacgg attgatgaag
   115201 gcgtccaacc tgacgccatt ctcatcccgc aacaaggcgt tagccgcaca ccgcgtggtg
   115261 atgcaaccgt gctgattgtt aacgataaaa gtcaggtgga agtgcgccct gtcgttgcca
   115321 gtcaggcgat tggcgataaa tggttgatta gcgaaggtct gaaatctggc gatcaagtca
   115381 ttgtcagcgg cctgcaaaaa gcgcgtccgg gagagcaggt taaagccact accgataccc
   115441 ccgcagatac tgcatcgaag taaggtaatc tgacatggca aactttttta ttcgacgacc
   115501 gatatttgca tgggtgctgg ccattattct gatgatggcg ggcgcactgg cgatcctaca
   115561 attgcccgtc gctcagtatc caaccattgc accgcctgcg gtttctgttt cagcaaacta
   115621 tccgggcgct gatgcgcaga ccgtgcagga tacggtgacg caggttatcg aacagaatat
   115681 gaacggtatc gataacctga tgtatatgtc ctccaccagc gattccgccg gtagcgtgac
   115741 aattaccctt accttccagt ccgggaccga tcctgatatc gcgcaagtgc aggtgcagaa
   115801 caaactccag ctcgccacgc cgttgctgcc gcaggaggtt cagcagcagg ggatcagtgt
   115861 tgaaaagtcc agtagcagct atttgatggt ggcaggcttt gtctctgata acccagacac
   115921 cacacaggac gatatctcgg actatgtggc ctctaacgtt aaagatacgc ttagccgtct
   115981 gaatagcgtc ggtgacgtac agcttttcgg cgcacagtat gcgatgcgta tctggctgga
   116041 cgccgatctg ctaaacaaat ataaactgac accggttgat gtgattaacc agttgaaggt
   116101 acagaacgct cagatcgctg ccggacagtt gggcggaacg ccagcgttac cagggcaaca
   116161 gttgaatgcc tcgattattg ctcagacgcg gttaaaaaat ccggaagaat tcggcaaagt
   116221 gaccctgcgc gtaaacagtg acggctcggt ggtgcgtctg aaagatgtcg cacgggttga
   116281 acttggcggt gaaaactata acgttatcgc tcgcatcaac ggaaaaccgg cggcgggcct
   116341 gggtattaag ctggcaaccg gcgcgaatgc tctcgatacc gcgaaagcca ttaaggcaaa
   116401 actggcggaa ttacagccat tcttcccgca gggaatgaag gttctctacc cttatgacac
   116461 cacgccattc gtccagcttt ctattcacga agtggtaaaa acactgttcg aagctattat
   116521 gctggtgttc ctggtgatgt atctgttctt gcagaatatg cgagcaacgc tgatccccac
   116581 cattgcggta cccgtggtgt tgttagggac gtttgccatc ctcgccgctt ttggttactc
   116641 catcaacaca ctaacgatgt tcgggatggt gcttgccatc gggctgctcg tcgatgatgc
   116701 gatagtggtg gtggagaacg tcgagcgcgt gatgatggag gataagctcc cgccaaaaga
   116761 agcgacggaa aaatcaatgt cgcaaataca gggcgcactg gtgggtatcg cgatggtgct
   116821 gtcagcggta tttattccga tggcattctt cggcggttct actggggcaa tttatcgtca
   116881 gttctctatc accatcgttt cggcaatggc gctttctgtt ctggtggcat tgattcttac
   116941 ccctgcgtta tgtgcaacac tgctcaaacc cacctcagct gagcatcacg aaaataaggg
   117001 tggtttcttc ggctggttta ataccacctt cgatcatagc gttaaccact acaccaacag
   117061 cgtcggcaaa atcctcggtt ccacaggacg atatttactg atctatgcgc tgattgttgc
   117121 aggaatggtg gtgttgtttt tacgtcttcc gtcttccttc ttacctgaag aggatcaggg
   117181 tgtctttctg accatgattc agttacccgc tggcgcgacg caagagcgga cgcaaaaagt
   117241 gttggatcaa gtgacggatt actatctgaa gaacgagaaa gcaaacgttg aaagtgtctt
   117301 tacggttaac ggctttagct tcagcggcca ggcacctccg caaccccatg ttttcacata
   117361 actgttgcgt tgaccaattg aatctacagg tatggccttc gtcagtctga aaccgtggga
   117421 agagcgtaat ggtgatgaaa acagtgcgga agcggtaatc catcgtgcca aaatggagtt
   117481 gggcaagatc cgcgacggtt ttgtcattcc attcaatatg ccagccattg ttgaactggg
   117541 cacggcaacg ggtttcgact ttgagttaat tgataaggct gggctgggtc acgatgccct
   117601 aacccaggcc cgtaaccagt tgcttggtat ggcggcgcaa catcctgcca gcttagtcag
   117661 cgtgcgtccc aatggcctgg aagacaccgc gcagtttaaa ctggaagttg accaggaaaa
   117721 ggcgcaggca ttaggtgttt cactttctga catcaatcag accatttcaa cggcgttggg
   117781 tgggacttac gttaacgact tcatcgaccg tggtcgcgtg aaaaaggtgt atgttcaggc
   117841 ggatgccaaa ttccgtatgc tgccagaaga tgtcgataaa ctttatgtcc gcagcaccaa
   117901 cggcgaaatg gtgccattct ctgcctttac cacttcacat tgggtgtatg gctctccgcg
   117961 actggaacgc tacaacggtc tgccgtcaat ggagattcag ggggaagccg cgccaggaac
   118021 cagttccggc gatgccatgg cgttgatgga aaacctagcg tcaaaattac ctgcgggcat
   118081 tggttatgac tggacgggta tgtcgtatca ggaacgctta tcgggaaacc aggctcccgc
   118141 tctggtagca atttcctttg tggttgtttt cctgtgcctt gctgcactct atgaaagctg
   118201 gtcaattcct gtctcggtta tgttggtcgt gccgttaggg attgtcggcg tgctgctggc
   118261 ggcgacactc tttaatcaaa aaaatgacgt ctactttatg gtaggcttgc taacgacaat
   118321 tggcttgtcg gccaaaaacg ctattttgat tgttgagttc gctaaagatc tcatggagaa
   118381 agagggtaaa ggtgttgttg aagcgacact aatggcagta cgtatgcgtc tgcgtcctat
   118441 cctgatgacc tctctcgcct ttattctcgg cgtattaccg ctggctatca gtaacggtgc
   118501 cggcagtggc gcgcagaacg cagtgggtat cggggtaatg ggaggaatgg tctctgcaac
   118561 gttgctggca atcttcttcg taccggtgtt ctttgtggtg atccgccgtt gctttaaagg
   118621 ataaatctga aacataaagg cgctttcggg tgcctttatg ttttccagtg aaacccataa
   118681 aaattaataa gatattcttt tgctcactct ttaaaagctt tctatagctc ccgctccctt
   118741 cactattttt acaattcaca taatttgtca ttattcattc cgcaagattt ataacctccg
   118801 gtgataaaat ggcattcagc tcgttaataa gagagttaac ttattaagcg ttagcgttta
   118861 ttactgaggt aacaccatga aaagattaat tcctgtggca ttgctcaccg cattgctggc
   118921 gggctgtgct cacgattcgc cctgcgttcc ggtttatgac gatcagggtc gtctggttca
   118981 caccaatacc tgtatgaaag gcacgactca ggataactgg gaaacggctg gggctattgc
   119041 tggcggggca gcggctgttg ctggcctgac gatggggatc atcgctctgt cgaaataact
   119101 gaacagggcg cggagtttcg cgctctgttt aacgatacag tatctttaca taagataaaa
   119161 tattgctata ttctgctggc taattctctg ctgatttagc atgttatatc gtgttatttc
   119221 gctgctttcg aaataacgca tcaatttaac tttcgcactt cccaccagaa atatactcac
   119281 gctaacaatc attattctgc ttagtaaaac acgcgtaaat gttttgcgcc aaaatatggc
   119341 gcatgtttca ttttcgcacc attgcggggc gctgttttta ttatttcctg tctacactca
   119401 gcttattgcg acgcgaaatc gtgcgcaaaa gctggcacta cttttgctta taagaggatg
   119461 gccacagaca ggcaaaagac gtttcccaaa cgtcctataa cgataatttt cgcctcacag
   119521 gaagcattat gaaaaagatg atgatagcca cactggctgc cgccagcgtg ctgcttgccg
   119581 ttgcaaatca ggcgcatgct ggcgcgacgc ttgatgccgt tcagaaaaaa ggttttgtgc
   119641 aatgcgggat cagtgatgga ttacctgggt tctcttatgc cgatgcagac ggtaagtttt
   119701 caggtattga tgttgatgtt tgtcgtggtg ttgccgctgc tgtatttggt gacgacacga
   119761 aagtgaaata taccccactc acagcaaaag aacgcttcac ggctttacag tcaggggaag
   119821 tggatttgct ctcccgtaat acgacctgga cttcatctcg cgatgccggg atgggaatgg
   119881 catttactgg cgtcacttat tacgacggca ttggcttcct gacgcacgat aaagcggggc
   119941 taaaaagcgc gaaagaactg gatggcgcta ccgtctgtat tcaggcgggt actgataccg
   120001 aactcaacgt cgccgactat ttcaaggcaa acaatatgaa gtacacacca gtgactttcg
   120061 atcgttctga cgaatcagcg aaggcactgg aatccggtcg ctgcgatacg ctggcttcgg
   120121 atcaatccca actgtatgcc ctgcgcatca aattaagcaa cccggctgaa tggattgtct
   120181 taccggaggt tatctctaaa gaaccgcttg ggccggtagt tcgtcgtggc gatgatgaat
   120241 ggttctcgat tgtacgctgg acgcttttcg ccatgctaaa tgctgaagag atgggcatca
   120301 attcccagaa cgtcgatgaa aaagcggcta atccagcaac gcctgatatg gcacatctgc
   120361 tgggtaaaga aggcgattac ggcaaggatc tgaagttgga taataaatgg gcctataaca
   120421 tcatcaaaca ggtgggtaac tactcggaaa ttttcgagcg taacgtaggt tcagaaagcc
   120481 cgctgaaaat taaacgtggg caaaataatc tctggaataa cggcggtatt cagtacgcac
   120541 cgcccgtgcg ttaagtcggt gtgaggtgac gagcgtcgct acggcggcgc tctcttcagc
   120601 gttatgcttt ccgaggtttc tatgtctcat cgccgctcaa ccgttaaagg ctcactctct
   120661 tttgccaacc ctacggttcg cgcctggtta ttccagatcc ttgccgttgt tgctgttgtc
   120721 ggcattgttg gttggttatt tcacaacact gtaacgaatc tcaataatcg tggcattact
   120781 tcaggttttg cctttctgga tcgcggcgct ggcttcggta ttgtccagca tttgatcgat
   120841 taccagcagg gcgacaccta cggacgcgtt tttattgtcg gcttactcaa tacgctactg
   120901 gtttctgcat tgtgtattgt gttcgcttct gttttgggct tctttatcgg tctggcgaga
   120961 ctttcggata actggctgct acgaaagctt tccacaattt atattgagat cttccgtaat
   121021 attcccccgc tgctgcaaat cttcttctgg tactttgccg tgttgcgcaa tttgcccgga
   121081 ccacgccagg ctgtgagcgc gtttgatctg gcctttttga gcaatcgtgg gctttatatt
   121141 ccgtcaccgc agctgggaga cggatttatt gcgtttatcc tggctgttgt tatggctata
   121201 gtcctttctg ttgggctatt ccgctttaat aaaacatacc agataaagac cggacagctg
   121261 cgccgcacct ggcccatcgc cgcagtgttg atcatcggtt tgcctttact ggcgcaatgg
   121321 ctttttggcg cagcactaca ctgggatgtc ccagccctac aaggctttaa cttccgcggc
   121381 gggatggttt taattcctga actggcagcc ttaacgctgg cactttcggt ttatacctct
   121441 gcatttatcg ccgagattat ccgcgcgggg atccaggcag tgccttatgg tcaacatgaa
   121501 gcggctcggt cactgggatt acccaacccg gttacgctac gccaggtcat tattccccag
   121561 gcactgcggg tgattattcc accgttaacc agccagtatc tcaacatcgt caaaaactcc
   121621 tctcttgccg ccgctattgg ctatcccgat atggtttcgc tgtttgctgg caccgtgctg
   121681 aaccagacgg ggcaagccat cgagacgata gccatgacca tgtcggtcta tctgattatc
   121741 agcctgacta tctcgctgct gatgaatatc tataaccgcc gcatcgcgat cgtggaacgc
   121801 taaggagtca tgatgacaaa agtattgctg tctcatccct cgcgcccggc gagccataac
   121861 tcaagccgcg cgatggtgtg ggtgcgaaaa aatctgttct ccagctggag caatagcctg
   121921 ctgactattg gctgcatatg gttgatgtgg gaactgatcc caccgttgct gaactgggca
   121981 tttttgcagg ctaactgggt tggctcaacg cgtgccgact gcacaaaagc cggtgcctgt
   122041 tgggtcttca tccacgaacg atttggtcag tttatgtatg gactttaccc acacgaccaa
   122101 cgctggcgaa ttaacttggc attactgatt ggccttgtgt caatcgcacc aatgttctgg
   122161 aaaatactcc cgcatcgcgg tcgctatatt gcggtctggg cagtgattta cccactgatt
   122221 gtctggtggc tgatgtatgg cggttttttt gctcttgagc gggttgaaac ccggcaatgg
   122281 ggcgggctga cgctaacttt aattattgca tcagttggga ttgcgggggc tctaccgtgg
   122341 gggatcttac tggcgttagg tcgccgctcc catatgccga ttgtgcgcat cttatcggtc
   122401 atttttatcg aattctggcg cggcgtgccg ctgattaccg ttctgtttat gtcttcggtc
   122461 atgctgcctt tgtttatggc agaaggcacc agtatcgaca aactgatccg cgcgctggtt
   122521 ggcgtgatcc tgtttcagtc agcatatgtt gcggaagtcg tgcgaggtgg attacaggcg
   122581 ctgcctaaag ggcaatatga agcggcagag tcgctggcgt tgggttactg gaaaactcag
   122641 gggctggtta ttctgccaca ggcgttgaag ctggtaattc ctgggctggt aaataccatc
   122701 atcgcactct tcaaagatac cagcctggtg atcattatcg ggttgttcga tcttttcagt
   122761 agcgttcagc aagcaaccgt tgatcccgcc tggttgggta tgtcgacgga agggtatgtt
   122821 ttcgccgcgc tgatctactg gatcttctgt ttcagcatgt cgcgctatag ccagcatctg
   122881 gaaaaacgtt ttaacaccgg gcgtacaccg cattgaggac actatgagcc aaattttact
   122941 gcaacctgct aacgcgatga ttacgctgga aaacgtcaat aaatggtatg gacaattcca
   123001 tgttttgaaa aatataaatt taaccgtgca accgggagaa cggatcgttc tgtgtggccc
   123061 ttcaggttcc ggtaaatcga caaccattcg ttgtattaat catctggaag aacatcaaca
   123121 gggacggatc gtggtagatg gcatcgaact taatgaagat atccgcaata ttgagcgcgt
   123181 caggcaggaa gtgggaatgg tctttcagca tttcaatctc ttccctcatc tgaccgttct
   123241 acagaactgt accctggcac cgatttgggt acgcaagatg ccaaagaaag aggctgaagc
   123301 tctggcgatg cattaccttg agcgggtgag aattgccgaa catgcgcata agtttcccgg
   123361 acagatttca ggtggtcagc agcaacgcgt tgccattgcg cgctcgctgt gtatgaagcc
   123421 gaaaattatg ttgtttgatg agcccacatc ggcgctcgat ccggagatgg tgaaagaggt
   123481 gctggatacg atgattgggc tggcgcaatc gggtatgaca atgttgtgtg taacccatga
   123541 gatggggttt gcgcgaaccg tcgctgaccg ggtgattttt atggatcgtg gcgaaatagt
   123601 ggagcaagct gcaccggatg agttttttgc gcatcctaag tcagagcgta cgagggcatt
   123661 tttatcgcag gtaatccatt aattgaatgt tagttcgaaa agcataaagg ccatcctttc
   123721 ggatggcctt tcgcttgatt tgatgtctgg cagtttatgg cgggcgtcct gcccgccacc
   123781 ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctggttggga
   123841 gagtgttcac cgacaaacaa cagataaaac aaaaggccca gtcttccgac tgagcctttt
   123901 gttttatttg atgtctggca gttccctact ctcgcatggg gagtccccac actaccatcg
   123961 gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg
   124021 ccgccagaca aattcttttc taatctgccg aactttaacc taaaaagtgg tgctgatacc
   124081 cagagtcgaa ctggggacct cacccttacc aagggtgcgc tctaccaact gagccatatc
   124141 agcacgct
//
LOCUS       DAZFTN010000002       101610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFTN010000002 DAZFTN010000000
VERSION     DAZFTN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 101610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            213..839
                     /gene="ydhY"
                     /locus_tag="ACHKV2_000123"
     CDS             213..839
                     /gene="ydhY"
                     /locus_tag="ACHKV2_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO6938094.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            860..2962
                     /gene="ydhV"
                     /locus_tag="ACHKV2_000124"
     CDS             860..2962
                     /gene="ydhV"
                     /locus_tag="ACHKV2_000124"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO6938095.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2975..3613
                     /gene="ydhW"
                     /locus_tag="ACHKV2_000125"
     CDS             2975..3613
                     /gene="ydhW"
                     /locus_tag="ACHKV2_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO6938096.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3677..4345
                     /gene="ydhX"
                     /locus_tag="ACHKV2_000126"
     CDS             3677..4345
                     /gene="ydhX"
                     /locus_tag="ACHKV2_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6938097.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4342..5127
                     /gene="phsC"
                     /locus_tag="ACHKV2_000127"
     CDS             4342..5127
                     /gene="phsC"
                     /locus_tag="ACHKV2_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO6938098.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5131..5943
                     /gene="ydhT"
                     /locus_tag="ACHKV2_000128"
     CDS             5131..5943
                     /gene="ydhT"
                     /locus_tag="ACHKV2_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO6938099.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5955..7559)
                     /gene="ydhS"
                     /locus_tag="ACHKV2_000129"
     CDS             complement(5955..7559)
                     /gene="ydhS"
                     /locus_tag="ACHKV2_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO6938100.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASNEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7685..7990)
                     /gene="ydhR"
                     /locus_tag="ACHKV2_000130"
     CDS             complement(7685..7990)
                     /gene="ydhR"
                     /locus_tag="ACHKV2_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO6938101.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8098..8174)
                     /locus_tag="ACHKV2_000131"
     tRNA            complement(8098..8174)
                     /locus_tag="ACHKV2_000131"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8138..8140),aa:Val,seq:gac)
     gene            complement(8179..8255)
                     /locus_tag="ACHKV2_000132"
     tRNA            complement(8179..8255)
                     /locus_tag="ACHKV2_000132"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8219..8221),aa:Val,seq:gac)
     gene            8563..9819
                     /gene="ydhQ"
                     /locus_tag="ACHKV2_000133"
     CDS             8563..9819
                     /gene="ydhQ"
                     /locus_tag="ACHKV2_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6938102.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9860..11233)
                     /gene="mdtK"
                     /locus_tag="ACHKV2_000134"
     CDS             complement(9860..11233)
                     /gene="mdtK"
                     /locus_tag="ACHKV2_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO6938103.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11448..12089
                     /gene="ribE"
                     /locus_tag="ACHKV2_000135"
     CDS             11448..12089
                     /gene="ribE"
                     /locus_tag="ACHKV2_000135"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO6938104.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(12129..13277)
                     /gene="cfa"
                     /locus_tag="ACHKV2_000136"
     CDS             complement(12129..13277)
                     /gene="cfa"
                     /locus_tag="ACHKV2_000136"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO6938105.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13568..14779)
                     /gene="punC"
                     /locus_tag="ACHKV2_000137"
     CDS             complement(13568..14779)
                     /gene="punC"
                     /locus_tag="ACHKV2_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO6938106.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14892..15824
                     /gene="punR"
                     /locus_tag="ACHKV2_000138"
     CDS             14892..15824
                     /gene="punR"
                     /locus_tag="ACHKV2_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO6938107.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15821..16846)
                     /gene="purR"
                     /locus_tag="ACHKV2_000139"
     CDS             complement(15821..16846)
                     /gene="purR"
                     /locus_tag="ACHKV2_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO6938108.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17145..17234
                     /gene="cydH"
                     /locus_tag="ACHKV2_000140"
                     /gene_synonym="ynhF"
     CDS             17145..17234
                     /gene="cydH"
                     /locus_tag="ACHKV2_000140"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO6938109.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17400..18569
                     /gene="ydhP"
                     /locus_tag="ACHKV2_000141"
     CDS             17400..18569
                     /gene="ydhP"
                     /locus_tag="ACHKV2_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6938110.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18715..19296)
                     /gene="sodB"
                     /locus_tag="ACHKV2_000142"
     CDS             complement(18715..19296)
                     /gene="sodB"
                     /locus_tag="ACHKV2_000142"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO6938111.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19424..20239)
                     /gene="mepH"
                     /locus_tag="ACHKV2_000143"
     CDS             complement(19424..20239)
                     /gene="mepH"
                     /locus_tag="ACHKV2_000143"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO6938112.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20574..20921
                     /gene="grxD"
                     /locus_tag="ACHKV2_000144"
     CDS             20574..20921
                     /gene="grxD"
                     /locus_tag="ACHKV2_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO6938113.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20972..25588)
                     /gene="lhr"
                     /locus_tag="ACHKV2_000145"
     CDS             complement(20972..25588)
                     /gene="lhr"
                     /locus_tag="ACHKV2_000145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO6938114.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25681..26328)
                     /gene="rnt"
                     /locus_tag="ACHKV2_000146"
     CDS             complement(25681..26328)
                     /gene="rnt"
                     /locus_tag="ACHKV2_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO6938115.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26431..26838)
                     /gene="gloA"
                     /locus_tag="ACHKV2_000147"
     CDS             complement(26431..26838)
                     /gene="gloA"
                     /locus_tag="ACHKV2_000147"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO6938116.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26919..28016)
                     /gene="nemA"
                     /locus_tag="ACHKV2_000148"
     CDS             complement(26919..28016)
                     /gene="nemA"
                     /locus_tag="ACHKV2_000148"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO6938117.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28053..28652)
                     /gene="nemR"
                     /locus_tag="ACHKV2_000149"
     CDS             complement(28053..28652)
                     /gene="nemR"
                     /locus_tag="ACHKV2_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO6938118.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28755..28994
                     /gene="ydhL"
                     /locus_tag="ACHKV2_000150"
     CDS             28755..28994
                     /gene="ydhL"
                     /locus_tag="ACHKV2_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO6938119.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29043..29939
                     /gene="ydhF"
                     /locus_tag="ACHKV2_000151"
                     /pseudo
     CDS             29043..29939
                     /gene="ydhF"
                     /locus_tag="ACHKV2_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            30020..30541
                     /gene="sodC"
                     /locus_tag="ACHKV2_000152"
     CDS             30020..30541
                     /gene="sodC"
                     /locus_tag="ACHKV2_000152"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6938120.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30542..32554)
                     /gene="ydhK"
                     /locus_tag="ACHKV2_000153"
     CDS             complement(30542..32554)
                     /gene="ydhK"
                     /locus_tag="ACHKV2_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6938121.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32554..33411)
                     /gene="ydhJ"
                     /locus_tag="ACHKV2_000154"
     CDS             complement(32554..33411)
                     /gene="ydhJ"
                     /locus_tag="ACHKV2_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6938122.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33414..33650)
                     /gene="ydhI"
                     /locus_tag="ACHKV2_000155"
     CDS             complement(33414..33650)
                     /gene="ydhI"
                     /locus_tag="ACHKV2_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO6938123.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33851..34285
                     /gene="slyA"
                     /locus_tag="ACHKV2_000156"
     CDS             33851..34285
                     /gene="slyA"
                     /locus_tag="ACHKV2_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO6938124.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34332..34799)
                     /gene="slyB"
                     /locus_tag="ACHKV2_000157"
     CDS             complement(34332..34799)
                     /gene="slyB"
                     /locus_tag="ACHKV2_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO6938125.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35072..36181
                     /gene="anmK"
                     /locus_tag="ACHKV2_000158"
     CDS             35072..36181
                     /gene="anmK"
                     /locus_tag="ACHKV2_000158"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO6938126.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36279..36608
                     /gene="mliC"
                     /locus_tag="ACHKV2_000159"
     CDS             36279..36608
                     /gene="mliC"
                     /locus_tag="ACHKV2_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO6938127.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36667..37323
                     /gene="pdxH"
                     /locus_tag="ACHKV2_000160"
     CDS             36667..37323
                     /gene="pdxH"
                     /locus_tag="ACHKV2_000160"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO6938128.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37452..38726
                     /gene="tyrS"
                     /locus_tag="ACHKV2_000161"
     CDS             37452..38726
                     /gene="tyrS"
                     /locus_tag="ACHKV2_000161"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO6938129.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38788..39648
                     /gene="pdxY"
                     /locus_tag="ACHKV2_000162"
     CDS             38788..39648
                     /gene="pdxY"
                     /locus_tag="ACHKV2_000162"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO6938130.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39692..40297)
                     /gene="gstA"
                     /locus_tag="ACHKV2_000163"
     CDS             complement(39692..40297)
                     /gene="gstA"
                     /locus_tag="ACHKV2_000163"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO6938131.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40403..41905)
                     /gene="dtpA"
                     /locus_tag="ACHKV2_000164"
     CDS             complement(40403..41905)
                     /gene="dtpA"
                     /locus_tag="ACHKV2_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO6938132.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42516..43151)
                     /gene="nth"
                     /locus_tag="ACHKV2_000165"
     CDS             complement(42516..43151)
                     /gene="nth"
                     /locus_tag="ACHKV2_000165"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO6938133.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43151..43846)
                     /gene="rsxE"
                     /locus_tag="ACHKV2_000166"
     CDS             complement(43151..43846)
                     /gene="rsxE"
                     /locus_tag="ACHKV2_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO6938134.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43850..44470)
                     /gene="rsxG"
                     /locus_tag="ACHKV2_000167"
     CDS             complement(43850..44470)
                     /gene="rsxG"
                     /locus_tag="ACHKV2_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO6938135.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(44474..45532)
                     /gene="rsxD"
                     /locus_tag="ACHKV2_000168"
     CDS             complement(44474..45532)
                     /gene="rsxD"
                     /locus_tag="ACHKV2_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO6938136.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45533..47851)
                     /gene="rsxC"
                     /locus_tag="ACHKV2_000169"
     CDS             complement(45533..47851)
                     /gene="rsxC"
                     /locus_tag="ACHKV2_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO6938137.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARLARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(47844..48422)
                     /gene="rsxB"
                     /locus_tag="ACHKV2_000170"
     CDS             complement(47844..48422)
                     /gene="rsxB"
                     /locus_tag="ACHKV2_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO6938138.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48422..49003)
                     /gene="rsxA"
                     /locus_tag="ACHKV2_000171"
     CDS             complement(48422..49003)
                     /gene="rsxA"
                     /locus_tag="ACHKV2_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO6938139.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(49080..49520)
                     /gene="ydgK"
                     /locus_tag="ACHKV2_000172"
     CDS             complement(49080..49520)
                     /gene="ydgK"
                     /locus_tag="ACHKV2_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6938140.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49606..49821)
                     /gene="ydgT"
                     /locus_tag="ACHKV2_000173"
     CDS             complement(49606..49821)
                     /gene="ydgT"
                     /locus_tag="ACHKV2_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO6938141.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50094..50219)
                     /gene="blr"
                     /locus_tag="ACHKV2_000174"
     CDS             complement(50094..50219)
                     /gene="blr"
                     /locus_tag="ACHKV2_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO6938142.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50462..51502
                     /gene="ydgJ"
                     /locus_tag="ACHKV2_000175"
     CDS             50462..51502
                     /gene="ydgJ"
                     /locus_tag="ACHKV2_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6938143.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51538..52539)
                     /gene="add"
                     /locus_tag="ACHKV2_000176"
     CDS             complement(51538..52539)
                     /gene="add"
                     /locus_tag="ACHKV2_000176"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO6938144.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(52643..53815)
                     /gene="malY"
                     /locus_tag="ACHKV2_000177"
     CDS             complement(52643..53815)
                     /gene="malY"
                     /locus_tag="ACHKV2_000177"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO6938145.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53825..55417)
                     /gene="malX"
                     /locus_tag="ACHKV2_000178"
     CDS             complement(53825..55417)
                     /gene="malX"
                     /locus_tag="ACHKV2_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO6938146.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55592..56620
                     /gene="malI"
                     /locus_tag="ACHKV2_000179"
     CDS             55592..56620
                     /gene="malI"
                     /locus_tag="ACHKV2_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO6938147.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56732..57499
                     /gene="hdhA"
                     /locus_tag="ACHKV2_000180"
     CDS             56732..57499
                     /gene="hdhA"
                     /locus_tag="ACHKV2_000180"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO6938148.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57728..58318
                     /gene="uidR"
                     /locus_tag="ACHKV2_000181"
     CDS             57728..58318
                     /gene="uidR"
                     /locus_tag="ACHKV2_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO6938149.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58709..60522
                     /gene="uidA"
                     /locus_tag="ACHKV2_000182"
                     /pseudo
     CDS             58709..60522
                     /gene="uidA"
                     /locus_tag="ACHKV2_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            60519..61892
                     /gene="uidB"
                     /locus_tag="ACHKV2_000183"
     CDS             60519..61892
                     /gene="uidB"
                     /locus_tag="ACHKV2_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO6938150.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61931..63195
                     /gene="uidC"
                     /locus_tag="ACHKV2_000184"
                     /pseudo
     CDS             61931..63195
                     /gene="uidC"
                     /locus_tag="ACHKV2_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(63240..64748)
                     /gene="ydgA"
                     /locus_tag="ACHKV2_000185"
     CDS             complement(63240..64748)
                     /gene="ydgA"
                     /locus_tag="ACHKV2_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6938151.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64849..66024)
                     /gene="manA"
                     /locus_tag="ACHKV2_000186"
     CDS             complement(64849..66024)
                     /gene="manA"
                     /locus_tag="ACHKV2_000186"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO6938152.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66223..67869
                     /gene="fumB"
                     /locus_tag="ACHKV2_000187"
     CDS             66223..67869
                     /gene="fumB"
                     /locus_tag="ACHKV2_000187"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6938153.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            68012..69415
                     /gene="fumC"
                     /locus_tag="ACHKV2_000188"
     CDS             68012..69415
                     /gene="fumC"
                     /locus_tag="ACHKV2_000188"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO6938154.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69412..70341)
                     /gene="tus"
                     /locus_tag="ACHKV2_000189"
     CDS             complement(69412..70341)
                     /gene="tus"
                     /locus_tag="ACHKV2_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO6938155.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70417..71718)
                     /gene="rstB"
                     /locus_tag="ACHKV2_000190"
     CDS             complement(70417..71718)
                     /gene="rstB"
                     /locus_tag="ACHKV2_000190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO6938156.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71722..72441)
                     /gene="rstA"
                     /locus_tag="ACHKV2_000191"
     CDS             complement(71722..72441)
                     /gene="rstA"
                     /locus_tag="ACHKV2_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO6938157.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72570..72905
                     /gene="ydgC"
                     /locus_tag="ACHKV2_000192"
     CDS             72570..72905
                     /gene="ydgC"
                     /locus_tag="ACHKV2_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO6938158.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72902..73624)
                     /gene="folM"
                     /locus_tag="ACHKV2_000193"
     CDS             complement(72902..73624)
                     /gene="folM"
                     /locus_tag="ACHKV2_000193"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO6938159.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73661..75043)
                     /gene="ydgI"
                     /locus_tag="ACHKV2_000194"
     CDS             complement(73661..75043)
                     /gene="ydgI"
                     /locus_tag="ACHKV2_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6938160.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75229..76173)
                     /gene="ydgH"
                     /locus_tag="ACHKV2_000195"
     CDS             complement(75229..76173)
                     /gene="ydgH"
                     /locus_tag="ACHKV2_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO6938161.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76697..78229
                     /gene="pntA"
                     /locus_tag="ACHKV2_000196"
     CDS             76697..78229
                     /gene="pntA"
                     /locus_tag="ACHKV2_000196"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO6938162.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIIFFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78240..79628
                     /gene="pntB"
                     /locus_tag="ACHKV2_000197"
     CDS             78240..79628
                     /gene="pntB"
                     /locus_tag="ACHKV2_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO6938163.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79653..80687)
                     /gene="tqsA"
                     /locus_tag="ACHKV2_000198"
     CDS             complement(79653..80687)
                     /gene="tqsA"
                     /locus_tag="ACHKV2_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO6938164.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            81099..81464
                     /gene="mdtJ"
                     /locus_tag="ACHKV2_000199"
     CDS             81099..81464
                     /gene="mdtJ"
                     /locus_tag="ACHKV2_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO6938165.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81451..81780
                     /gene="mdtI"
                     /locus_tag="ACHKV2_000200"
     CDS             81451..81780
                     /gene="mdtI"
                     /locus_tag="ACHKV2_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO6938166.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81819..82640)
                     /gene="ydgD"
                     /locus_tag="ACHKV2_000201"
     CDS             complement(81819..82640)
                     /gene="ydgD"
                     /locus_tag="ACHKV2_000201"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO6938167.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82740..82823)
                     /locus_tag="ACHKV2_000202"
     CDS             complement(82740..82823)
                     /locus_tag="ACHKV2_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938168.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82916..83224)
                     /gene="asr"
                     /locus_tag="ACHKV2_000203"
     CDS             complement(82916..83224)
                     /gene="asr"
                     /locus_tag="ACHKV2_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO6938169.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            83495..83599
                     /locus_tag="ACHKV2_000204"
     CDS             83495..83599
                     /locus_tag="ACHKV2_000204"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO6938170.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(83648..84901)
                     /gene="ynfM"
                     /locus_tag="ACHKV2_000205"
     CDS             complement(83648..84901)
                     /gene="ynfM"
                     /locus_tag="ACHKV2_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6938171.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            85008..85901
                     /gene="ynfL"
                     /locus_tag="ACHKV2_000206"
     CDS             85008..85901
                     /gene="ynfL"
                     /locus_tag="ACHKV2_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6938172.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKHFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            86036..87256
                     /gene="mlc"
                     /locus_tag="ACHKV2_000207"
                     /gene_synonym="dgsA"
     CDS             86036..87256
                     /gene="mlc"
                     /locus_tag="ACHKV2_000207"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO6938173.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            87381..88076
                     /gene="bioD"
                     /locus_tag="ACHKV2_000208"
     CDS             87381..88076
                     /gene="bioD"
                     /locus_tag="ACHKV2_000208"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6938174.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(88029..89321)
                     /gene="clcB"
                     /locus_tag="ACHKV2_000209"
     CDS             complement(88029..89321)
                     /gene="clcB"
                     /locus_tag="ACHKV2_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO6938175.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(89479..90093)
                     /gene="dmsD"
                     /locus_tag="ACHKV2_000210"
     CDS             complement(89479..90093)
                     /gene="dmsD"
                     /locus_tag="ACHKV2_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO6938176.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(90136..90990)
                     /gene="ynfH"
                     /locus_tag="ACHKV2_000211"
     CDS             complement(90136..90990)
                     /gene="ynfH"
                     /locus_tag="ACHKV2_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO6938177.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(90992..91609)
                     /gene="ynfG"
                     /locus_tag="ACHKV2_000212"
     CDS             complement(90992..91609)
                     /gene="ynfG"
                     /locus_tag="ACHKV2_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO6938178.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(91620..94043)
                     /gene="ynfF"
                     /locus_tag="ACHKV2_000213"
     CDS             complement(91620..94043)
                     /gene="ynfF"
                     /locus_tag="ACHKV2_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO6938179.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(94104..96530)
                     /gene="ynfE"
                     /locus_tag="ACHKV2_000214"
     CDS             complement(94104..96530)
                     /gene="ynfE"
                     /locus_tag="ACHKV2_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO6938180.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(96729..97034)
                     /gene="ynfD"
                     /locus_tag="ACHKV2_000215"
     CDS             complement(96729..97034)
                     /gene="ynfD"
                     /locus_tag="ACHKV2_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO6938181.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            97142..97852
                     /gene="ynfC"
                     /locus_tag="ACHKV2_000216"
     CDS             97142..97852
                     /gene="ynfC"
                     /locus_tag="ACHKV2_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO6938182.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(97855..98415)
                     /gene="speG"
                     /locus_tag="ACHKV2_000217"
     CDS             complement(97855..98415)
                     /gene="speG"
                     /locus_tag="ACHKV2_000217"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO6938183.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(98450..98791)
                     /gene="ynfB"
                     /locus_tag="ACHKV2_000218"
     CDS             complement(98450..98791)
                     /gene="ynfB"
                     /locus_tag="ACHKV2_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO6938184.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            98926..99252
                     /gene="ynfA"
                     /locus_tag="ACHKV2_000219"
     CDS             98926..99252
                     /gene="ynfA"
                     /locus_tag="ACHKV2_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO6938185.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            99458..99544
                     /locus_tag="ACHKV2_000220"
                     /pseudo
     CDS             99458..99544
                     /locus_tag="ACHKV2_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(100019..100206)
                     /locus_tag="ACHKV2_000221"
                     /pseudo
     CDS             complement(100019..100206)
                     /locus_tag="ACHKV2_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(100241..100492)
                     /locus_tag="ACHKV2_000222"
     CDS             complement(100241..100492)
                     /locus_tag="ACHKV2_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6938186.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQTSARPRRHLT"
     gene            complement(100565..>101610)
                     /locus_tag="ACHKV2_000223"
     CDS             complement(100565..>101610)
                     /locus_tag="ACHKV2_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6938187.1"
                     /translation="ELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIF
                     DGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTST
                     SRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDT
                     MKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRR
                     SYERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVS
                     AIWQKLIPSQADS"
BASE COUNT        25439 a        25021 c        26249 g        24901 t
ORIGIN      
        1 ataatgatca atgttaagag cccagaataa ccacattatt ttttctccta accataaagg
       61 attggtttat cggcgttaaa tattttctca tacactatcg ggttagggaa aatatctggt
      121 gttggggaca gagtcagcag cgtattagct gaatgcttaa tatcaggctg ctgaattgtc
      181 agcactgtgt ttgcgaagag gatgcgaaat aaatgaaccc ggttgatcgt ccactattag
      241 atattggctt aacgcgatta gaattcttgc gtatatcagg aaaaggcctt gctggtctaa
      301 caattgcccc tgcgttactg tcacttttgg gttgcaaaca ggaagatatt gatagcggca
      361 cagtagggtt gataaataca cccaaagggg tgctggtgac ccagcgagcg cgatgcacgg
      421 gttgccaccg ctgtgaaatc tcttgtacca acttcaacga tggctcagta gggacattct
      481 tctcccgtat caaaatccat cgcaattatt tctttggcga caacggggtt ggctctggcg
      541 gcggcctgta tggcgatctc aactataccg cggacacctg ccgtcaatgc aaagagccgc
      601 aatgcatgaa cgtctgcccg attggtgcga ttacctggca gcagaaagaa ggctgtatta
      661 ccgtcgatca taaacgttgt attggctgta gcgcctgtac tacggcatgc ccgtggatga
      721 tggccaccgt aaataccgaa agtaaaaaat cctcgaaatg tgtgttatgc ggtgaatgcg
      781 caaacgcctg cccaacaggg gcgttaaaaa ttatcgagtg gaaagatatt actgtgtgaa
      841 tcttgcaaag gaaaaaatca tgactaacgg ttggacaggt aatatattaa gagtcaatct
      901 cacgacagga aatattaccc tcgaagattc cagtaagttt aaaagttttg tcggtggcat
      961 gggcttcggc tacaaaatta tgtatgacga agtaccgcca ggcacgaaac ctttcgatga
     1021 agcgaataaa ttagtctttg ctaccggccc attaactgga tctggtgccc cctgtagttc
     1081 tcgcgtaaat atcacctcac tttctacttt taccaaagga aatttagtcg tcgatgccca
     1141 tatgggtggc ttttttgcag cgcaaatgaa attcgctgga tacgacgtca ttattatcga
     1201 agggaaggcg aaatcaccgg tatggctgaa gattaaagat gacaaagtta gcctggaaaa
     1261 agcagatttc ttatggggaa aagggacgcg cgcaacgacg gaagaaattt gtcgcttgac
     1321 cagtccggaa acctgtgtgg cggctattgg tcaggctggg gaaaaccttg ttcctctctc
     1381 tggcatgttg aatagccgta accacagcgg cggtgcggga actggcgcaa taatgggttc
     1441 gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg gtcaacattg ccgatcgtca
     1501 ggagatgaag cgtctcaatg attacatgat gactgaactt atcggtgcga ataacaacca
     1561 tgtcgtgcca agtacgccgc aatcgtgggc agagtattca gatcccaagt cacgctggac
     1621 agcacgtaaa gggctgtttt ggggcgcggc tgaaggcggt ccaattgaaa cgggtgaaat
     1681 tccgccaggc aatcagaata cggtcggctt tcgtacctat aaatccgttt ttgacttagg
     1741 tccggcagca gagaaataca cagtaaaaat gagcggctgc cactcttgcc cgatccgttg
     1801 tatgacccaa atgaatattc ctcgggtgaa agagtttggc gtgcccagca caggtggtaa
     1861 cacttgtgta gcaaactttg tccataccac catcttcccg aacgggccga aagattttga
     1921 agataaagac gatggtcgtg tgattggtaa cctggttggt ctgaatttgt tcgatgacta
     1981 cggcctatgg tgtaactacg ggcagttgca tcgcgacttt acttattgtt acagcaaagg
     2041 tgtgttcaag cgtgttctgc ctgctgaaga gtatgcagaa attcgctggg atcaactgga
     2101 agcgggagac gttaacttca ttaaagattt ttactaccgt ctggcgcatc gtgtgggtga
     2161 gctgagtcac ctggctgatg gttcatatgc catcgcagaa tgctggaatt tgggtgaaga
     2221 gtactggggc tacgcgaaaa ataaactctg gtcgccgttt ggctatccgg ttcaccatgc
     2281 caatgaagcg tcagcgcagg tcggttccat tgttaactgt atgttcaacc gtgactgcat
     2341 gacgcatacc catatcaact ttattggttc cggcttgcca ttgaaactgc aacgtgaagt
     2401 ggcgaaagaa ctttttggtt ctgaagatgc ttacgatgaa accaaaaact acacgccaat
     2461 caatgacgca aaaattaaat atgctaagtg gtcgctgttg cgggtctgtt tgcataacgc
     2521 cgtcaccctg tgcaactggg tctggccaat gaccgtttcg ccgctgaaaa gccgtaatta
     2581 ccggggcgat ctggcgcttg aagccaaatt cttcaaagcg atcactggcg aagagatgac
     2641 tcaggaaaaa ttagatttag ctgcagagcg tatctttaca ttgcatcgtg cctacacggt
     2701 gaaactgatg caaaccaaag atatgcgtaa tgaacacgat cttatctgtt cctgggtatt
     2761 cgacaaggat ccgcagatcc cggtctttac tgaaggtact gacaaaatgg atcgtgatga
     2821 tatgcatgcc tcgctcacga tgttctacaa agaaatgggc tgggacccac agcttggttg
     2881 tccgacccgc gaaacattgc agcgtctggg gctggaagat attgccgccg atctggcagc
     2941 acacaatcta ctgcctgcgt aaggaatggg taagatgaac catcaggatg aattgccgtt
     3001 agcgaaagtc agcgaagttg acgaggcgaa gcggcaatgg ttgcagggga tgcgtcaccc
     3061 cgttgatacg gtaaccgaac cagaaccggc tgaaatcctg gcagagttca ttcgccagca
     3121 ttctgtggct gggcagctgg tggcgcgcgc cgtgtttctc tcgccaccct attcagttgc
     3181 agaagaggaa ctgtcagttt tgctggaaaa catcaagcag aacggagact acgcggatat
     3241 cgcttgtatg acgggaagtc aggatgatta ctattactca acgcaggcca tgagtgaaaa
     3301 ctacgcggcg atgtctttgc aagttgtgga gcaggacatt tgtcgcgcca ttgcacacgc
     3361 cgttcgtttt gagtgccaga cgtatcctcg tccttacaaa gtcgccatgc tgatgcaagc
     3421 gccatattac ttccaggaag cgcagattga agccgcgatc gccgcaatgg atgttgcacc
     3481 tgaatatgcc gatatacggc aggtagagtc atcgacggcg gtgttgtact tgtttagcga
     3541 gcgatttatg acctatggca aggcttatgg cttatgcgaa tggttcgagg tagagcagtt
     3601 tcagaacccc tgaaatttca gggagatgtc atggataggg tggacagttg cagccactgc
     3661 attaggggat aatcagatgt ccttcactcg acgcaaattt gttctgggga tgggaacagt
     3721 aatctttttt actggttctg cgtcttctct gttagcgaac acgaggcaag aaaaggaagt
     3781 tcggtatgcc atgattcatg acgagtcacg gtgcaatggc tgtaatattt gcgcccgcgc
     3841 atgccgtaaa actaaccacg cccccgctca gggaagccgt ttatcgatag cacacattcc
     3901 agtcaccgat aacgacaacg aaacgcagta tcactttttt cgccagtcat gtcagcactg
     3961 cgaagatgct ccgtgcatcg acgtatgccc gacaggggcg tcgtggcgtg atgagcaggg
     4021 gatcgtgcgg gtagaaaaat cgcagtgcat cggctgcagt tattgcattg gcgcttgtcc
     4081 gtaccaggtt cgctacctta accctgtcac taaagtggca gataaatgtg atttctgcgc
     4141 tgagtcccga ctggcaaaag gctttccgcc catttgcgtc agcgcttgcc cggaacacgc
     4201 attgatcttt ggtcgcgaag atagccctga aattcaggcg tggttgcagg ataataaata
     4261 ctatcaatat cagctacctg gtgccgggaa accgcatctg tatcgccggt tcggtcaaca
     4321 tttgattaaa aaggaaaatg tatgaacccg tcgcaacatg ctgaacagtt tcagagccag
     4381 ttagcgaact atgtgccaca gttcactccc gaattttggc cggtgtggtt gatcattgcc
     4441 ggagtattgc tggttgggat gtggttggtg ctggggctgc acgccttgct tcgtgctcgt
     4501 ggcgtgaaga aatcagccac cgatcatggt gagaggattt atctttacag caaagcggtc
     4561 agattatggc actggtcgaa tgcgttactc tttgtattgt tgctggcaag tgggctgata
     4621 aatcactttg cgctggtggg cgcaactgcg gttaaaagtc tggttgcggt gcatgaagtt
     4681 tgcggatttt tgttactggc atgctggctc ggctttgttc tgatcaatgg cgttgggggt
     4741 aatggccacc actatcgcat tcgtcgtcag ggttggctgg agcgggcggc aaaacaaacg
     4801 cgattttatt tgtttggcat tatgcagggg gaagaacatc ctttcccggc aacaacccag
     4861 tctaaattta atcccttaca gcaggttgcc tatgttggtg tcatgtatgg attgctgccg
     4921 ttgttactat tgacggggct gctgtgtctc tatccgcaag ccgtgggaga tgtgtttcct
     4981 ggcgtaagat actggttatt gcaggcgcat tttgctctgg catttataag cctctttttt
     5041 atcttcggtc atctttatct ttgcaccacg gggcgtacgc cacacgaaac ctttaaaagc
     5101 atggtcgatg gctatcaccg gcactaacga atgatcatta cacgagcaga cctgcgagaa
     5161 tggcgtatcg gagcggtgat gtatcgctgg ttcctgcgcc atttcccacg cggcggtagt
     5221 tatgccgata ttcatcatgc actgattgag gaaggatata cggactgggc ggagagtctg
     5281 gttgagtatg cgtggaaaaa atggctggcg gatgaaaact tcgcgcatca ggaagtttcc
     5341 tctatgcaaa aactggccac agaccccgga gagagagcat tttgcagtca gtttgcccgc
     5401 agcgacgatc atgctcgcat cggctgttgt gaggataacg ccagaatcgc aaccgctggg
     5461 tatgctgcac aaattgccag tatgggttat agcgtgcgga ttggaagtgt cggctttaac
     5521 agtcacattg gcagctccgg tgagcgcgca cgggttgctg taaccggcaa ttcttcgcgg
     5581 ataagcagcg ctggagatag cagtcggatt gcgaataccg gaatgcgagt acgcgtttgt
     5641 acattgggta aacggtgtca tgtcgccagt aatggtgacc tggtgcaaat cgccagcttt
     5701 ggcgcaaacg ccagaattgc caacagcggc gacaatgtac acatcattgc cagcggtgaa
     5761 aattcaacgg tagtgagcac gggcgtggtc gactcaatta ttctcgggcc agggggcagc
     5821 gccgcactgg catatcatga cggcgaaaga gttcgcttcg ccgtcgccat tgaaggtgaa
     5881 aacaatattc gagccggagt ccgctatcgt cttaatgaac agcatcagtt tgttgagtgc
     5941 tgatagcttt acctttaatc aaacgaaaga cgccgacgag cacgggatgc aggctttacg
     6001 accgcccgag ccatcgcctc accaatttct gcacatgccg taagtccctg aacgaaaggc
     6061 tggtcgtgca tcaaccaggg caacgcgccg aacgctacgc gcccacgaat atcttcgggt
     6121 tgctgtaacg tataatcttc accaacatca gggatttcat cccctgtttt ctgtaattgt
     6181 tcgcgtagtc cagggaaagg aatatctttc actttaagcg gacgctgccc gcgggcatca
     6241 ataaaaacgt caaacgagta gctgttgtct tccgttttca ggacggtgcg cgactcgtta
     6301 atttccattt tgtagtcttc accgagagcg agaatatgaa tgattcccgc ttcacgtaag
     6361 gcaagtaggc gacgaataga ctctgaaggg attgccgcat aattatcgat gaaaacccgg
     6421 gcaaggcctt tactgaaccg tttatggtcg tgttcattca gatgtggaac aatttcctgt
     6481 acggcttcat gcaggcgcag aatgacataa cgccagggaa cagtatgttt ttctcgttta
     6541 ttgcgttcga cttcctggag atttttttct gcccagtcaa attggtcgcg ttgtttgcgc
     6601 tcggcaaacc aggcttgagc aaaggaatcg acattcaggc tctctaaggc tatgcgttga
     6661 ctccagtctg gatcagcaaa cttgatttcc tctactatca atctaaatac ccgatccaaa
     6721 aggccatatt cgcctttttg aatctcagca tttaatgcct gatcagtgac gatgtgtaag
     6781 ggctcgtagg gaatagggca atagaaatcg gcttcgggta aaatacccgt gcgcgacatt
     6841 aacgtaatat ttagcttttc acttgcgtta tcgcggtgaa aaacgacgtg ttgtttatca
     6901 tcttcaatga acgaaccatg ctgaatagcc actgccattg ccgcatccag tccgctcaag
     6961 gatgttccca taatacccac gttacacgca tcgacctttg cttccatcaa gcctgaccac
     7021 gggcttggaa aatacgttcg ggttgcttct tcttcatcag gccagacgtg acccgtggcg
     7081 atcaccgcta aatcaaacgt ctcgctgggt aaatcctgat ttgtagcgag catgacgcca
     7141 gcatttgtaa tttgcagatc ggtaacctgg catgattcat aaacagccac tgcaaatttt
     7201 tgctttcgtg cctggtctac taatcgtaaa aattgatcgc ggaaatattc gcccagcaga
     7261 attcgcggta aaaactgacg atcgtgcaag gtttcttttt taacgccata acgctggaga
     7321 tgactggctt cttgcttttg tagccattcg agatacgtac aataaatcgg cggtatttca
     7381 ttactggcaa tatttgccag catcattttt gagttttcct catcactgta tggcatcccg
     7441 acaccggcct cgtcagcctg ctcgaagata gaaatagaaa gtggagtttg ttgctgtaga
     7501 agcgagaata aggtgtagat ccccgtaggc ccggcaccca caattgcaat ttttttcatt
     7561 gttacttacc tgtacgcatt ctttcgaatt tagtgttatc agataagttt ggttgagatt
     7621 tttagaattt gagaaatttc agacagggat gaaaagggcg ctaaccgccc ttgattaaat
     7681 tctgtcaggc gagttttgcc tgattgattt gactcagtgg ctcattgaca tcgaaaactt
     7741 tggcgacaac ttcctcaacg ccgagttttt tcagttgggc agtgtgtttt tccagatagg
     7801 caagggcgct tttttcatca gtgaaaaggt agatcccacc ggcttcgtgg ttcttttcac
     7861 tttctgtcca taccttccac agaaaaccag gttcctgatt aatcgactct gcaagtggtt
     7921 taagctgctc agccattgcg tcgccaaatg ggccattaaa agcaaaatga agttgtaaca
     7981 aagttgccat agcgattctc cttctgataa cgcatacgaa gacagtgtaa atcttttaac
     8041 gtattggata cagagaagaa gcagagggaa aaaggagaag accagattaa caaaatctgg
     8101 tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc aaggtggtgc tctaaccaac
     8161 tgagctacgg acgcaggatg gtgcgttcaa ttggactcga accaacgacc cccaccatgt
     8221 caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt tgtaggtgac aacggggacg
     8281 aatattagcg gcagagtggg aaggtggcaa gaggcaaaac gtaattttct gcgctatttc
     8341 gaccgtttgc agagctttta agcaaattgg ctatattttg ttgatttgca agggcgattt
     8401 ttattcagga tcgcatttac atctgataca acccaataat tcgtgtatca gagtttaaac
     8461 agagttgttt gccaccatct caaatctgtc gtggataaat cctccgtagc ctttacactt
     8521 aggtagcaat aaaggcacac agatgcagca aaggaataaa tcatgggatc tgatgcgaaa
     8581 aacttgatga gcgacgggaa tgtgcaaatt gttaagaccg gcgaggtcat tggcgcgacg
     8641 caacttactg aaggcgagtt gattgttgaa gctggcggaa gagccgaaaa taccgtggtc
     8701 acgggggctg gctggttgaa agtggcaacc ggtgggatcg ccaaatgcac acagtacggt
     8761 aacaatggca cgctatcggt cagcgacggt gccattgcca cagatattgt tcagtccgag
     8821 ggaggcgcaa ttagtctctc tacgctcgct acggttaatg gccgccatcc cgaaggtgaa
     8881 ttcagcgttg atcagggtta tgcctgcggt ttgttgctgg aaaatggcgg taacctgcgt
     8941 gtactggaag gacatcgcgc ggaaaaaatc attctcgatc aagagggcgg cctgttggtt
     9001 aatgggacaa cctcagcggt cgtggtagat gaaggtggtg aattgttggt gtatccaggt
     9061 ggggaagcca gcaattgtga gattaatcag ggcggcgttt ttatgctggc ggggaaagcc
     9121 aatgatacgt tgcttgctga tggcaccatg aataatctcg gtggtgaaga ctctgacact
     9181 attgttgaga atggagccat ctatcgtctg gggacggatg gtcttcagct ctacagttcc
     9241 ggtaagacgc aaaacctgtc cgttaatgtg ggtggtcggg ctgaagtgca tgccggtacg
     9301 ctggaaaatg cggtaataca aggtggaaca gtgatcctgt tgtcacccac cagcgcggac
     9361 gaaaattttg tcgtagagga agatcgcgca ccggttgaac tgaccgggag tgttgcatta
     9421 ctggacggcg cttcaatgat tattggctat ggcgcagatc tgcaacaatc aacgattact
     9481 gtacagcagg gcggtgtatt gattctcgac ggcagtacgg taaaaggtga cagtgtcact
     9541 ttcagtattg gtaacatcaa tctgaatggc ggaaaactgt ggctgatcac tggtgcggca
     9601 acgcatgtgc agctgaaagt gaaacgcctg cgcggagagg gagcgatttg cctgcaaacc
     9661 agtgcgaaag aaatttcacc tgacttcatc aatgtgaaag gggaagttaa cggggatata
     9721 cgcgttcaga taacagatgc cagtcggcaa actctgtgta acgcactgaa actacagcca
     9781 gacgaagacg ggattggcgc aacgctccag cctgcgtaaa aacacaggcg caccccgtga
     9841 aggttgcgcc ttgtctttat tagcgggatg ctcgttgcag aatgatggct gacggcagac
     9901 gttgcaggta ccgcatacgc agcatcatca taatggctgc cgacgtcagg ccaataataa
     9961 agcctatcca gaagcctgct ggccccatag gttcaacgac cagatcggtc agtgccagaa
    10021 tatagccgct tggcaagccc agcacccagt aagccgtaaa ggtgatatag aaaatggaac
    10081 gcgtatcttt ataaccacgc aaaatcccac tgccaatcac ctggattgag tcagaaatct
    10141 gatataccgc cgccagcaac atcaaatgcg ccgccagcgt tacaacctcg ggattgtcgt
    10201 tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa tatggccgtc agggttgcca
    10261 tacagacacc caccataagc ccggtccgcg cagcggtttg cgcatccagc gttgagccct
    10321 gacccagacg ataacctacg cggatagtta ccgctgccgc cagcgacatt ggaagcacga
    10381 acattagtga actaaagttc agggcaatct ggtgtcctgc gacatcaaca ataccgagcg
    10441 gagacactaa cagagccacg acggcaaaca gtgtcacttc aaagaatagt gccagcgcaa
    10501 tcggcaaacc gagttgaatc agtcgtttca taaccgcagg atcgggtttc gcggtgcctt
    10561 tttcgttacg aatatcgcgc atcgagcggg cgcgtttaat gtaagaaacc atggcaagga
    10621 acatgaccca atacaccgcc gcagtagcca cgccacaacc aacgccaccg agctcaggca
    10681 taccgaaatg accataaata aagatatagt tcaccggaat gttcaccagc aggccgataa
    10741 agcccattac catacccggc ttggtttttg ccagaccttc acattggtta cgggcaacct
    10801 ggaagaaaag atagcccggc gcgccccaca gcaacgcacg cagataaccc acggctttgt
    10861 ccgccagagc cggatcgatg ttttccatgg agcggataat gtaacctgca ttccacagca
    10921 ccagcataat gagaacggaa acaaaacccg ccagccagaa gccctgtcgc acctgatgcg
    10981 caatgcgctc acgtcgaccg gaaccattta attgcgcgat aaccggcgtt aatgccagca
    11041 gcagtccgtg accaaagagg atcgccggaa gccagataga agtaccgata gcgaccgccg
    11101 ccatgtcggt ggcactatag ccgcccgcca tcacggtatc gacaaaaccc atcgcagttt
    11161 gggcgatttg cgcgagaatc accgggattg ctaatgctaa taacagacgc gcttcactga
    11221 tatacttctg cacgtgaaca ccttttattt gtagttatat gagagactaa aaagccgcct
    11281 gaataggcag caaaaagaag aagcagggga aattcagtct attgtagcga ggatttatta
    11341 attctccagt gaaaaaatag ccattcttac cccttcactg gtaagggcgt tttccacctg
    11401 ctattgtgct gaacagaatg tcttaactga tttcaggagt tgtaagtatg tttacgggga
    11461 ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa accaaatttt cgcacacatg
    11521 tggtggagtt acccgaccac atgctggacg gtctggaaac cggtgcctcc gtggcgcata
    11581 acggttgctg cctgaccgtg acggaaatta acggcaacca tgtcagtttt gacctgatga
    11641 aagaaacgtt gcgcattacc aatcttggcg atttaaaagt gggggattgg gtaaacgttg
    11701 agcgtgcggc gaaattcagt gatgaaattg gcggacactt aatgtcaggt catattatga
    11761 ccaccgctga agtggcgaaa atattaacct cggaaaataa tcgccagatc tggtttaaag
    11821 tccaggatag tcagttgatg aaatatatcc tgtacaaagg atttattggc atcgacggta
    11881 ttagcctgac cgtcggcgaa gtcacgccaa cgcgtttttg cgtccattta attccggaaa
    11941 ctctggaacg cacgactctt gggaagaaaa aacttggcgc acgcgtcaac attgaaatcg
    12001 atccacaaac tcaggcagtg gtagatacgg tagaacgtgt gctggcggca cgagaaaatg
    12061 ccatgaatca atcaggtaca gaagcctgat ctgaaatcgc ccccggagag ggggcgatag
    12121 aatagccttt agcgagcgac tcgaaggccg ttttcaacac cgcgtgagaa caccacctgc
    12181 cagagctgaa tatcacgggc gcggaaagca cctgcgcagg cattcagata ataggtaaac
    12241 atccgtttaa agcgttcact gtagttatcc gcaatttctg gccatgcggc gacgaatcgt
    12301 tcataccacg ccatcaacgt agtatcgtaa tcagcaccga agttatgcca gtcttccatc
    12361 acaaagtggg gttcgctgga ctgagcaatc tggcgtacag agggcaggca accgttcggg
    12421 aaaatatatt tattaatcca gggatcaaca ttcagatcgg tttttttcga accgatagta
    12481 tggagcagga atatgccttc cggtttcaaa ttacgatcca ccaccgtaaa ataggtatcg
    12541 taatttttcg gtccgacgtg ctcgaacatc cccacagaaa caatacgatc aaactggtcg
    12601 ttcaggtcac gataatcttg cagcaaaatg gtgacatcca ggccttcaca gcgttcctga
    12661 gccatttttt gctgttcggc agaaatggtg acgcccacca cgcttacgtc ataattagat
    12721 gccatgtagt gtgccagtcc gccccagccg cagccaatat ccagtacgcg catcccaggt
    12781 tttaactgca atttttcaca aatcattttg agcttcgcct gctgggcaga ttccagatta
    12841 tcggcatctt tccagtaagc gcaggaatat tgcatgaagg gatcaagcat gcggctgaac
    12901 aagtcattac ccaaatcgta atgctctttg ccgactatcc aggcacgttt tttactctgc
    12961 agattgaaga gacgggcgcc ggcaatacgc agcgtgtctt tgaaatgatg ggggagttgg
    13021 ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat ccagtcggtc acattcccac
    13081 cagccatcca tataactttc gcctaacccc aaagagcctt cttgcagaac gcgtttaaaa
    13141 aaatcggggt ttttcacacg aatatccgcc ggggcagaac cgttaatggc tataccggca
    13201 cggctaagta attcgttggc gatacggtac cagttgtcat ccggtacact gacttcttct
    13261 atacacgatg aactcatagt ttctccatca ctggtcggtg atcagaacct taaaacagcg
    13321 tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa accgcgagtc tataatccga
    13381 cgtagaacag agggaaagga ggaacccttg ccgaaatgac catccatagt gagtcgggaa
    13441 cgatcctgtc cccgtaaaaa cataatattt atcgtagctt taacaactcg aaattagtat
    13501 aggccgctaa tttcaatcat acaacaaaat tttgttagca gcctaagtat aagtatatcg
    13561 atatagatca gtgtgattcg ctatgagcga cttcggcatt gccatgattc tggcagccaa
    13621 cttcttcaca acgttgcatc atgtaaccca gcgcgaccag cactactgtt gataacatca
    13681 cgctggtggt ggtgagcaat ggcgtgctga tactgatcag ccaggaaact accagacttg
    13741 cgaggaagca cagacccagt tgcagagtgt tctgcaacgc tgcggcgcga ccagttgcgt
    13801 gtgggaaggg acgcagagcc tgggcgacaa caatagggta gatcgcgcca ttagcaatcg
    13861 ccatcacaca gaatgggatc aggatttcga ccagcgacac atggctaatg aagcccgcag
    13921 cccaggtcgc aatgacgctg acagcaaaca gcaccagcaa ccacggtaat aactgcttgc
    13981 cttgccattt ctgcagcgcg gcgcgacagc cataaccacc aatcagaaac gcgatagttt
    14041 gcgggacata acttaaacca ataactgccg ggctgtagcc catttcacta aggatgaacg
    14101 gtgaaccggt cagccatgca aaaaaactgg ctgaacaggc cgcgtatatc agcacattgc
    14161 cgcgataggt tttagaacgt agcaggtcgg taaaggtcag accatcctga ctattgttac
    14221 gggccttcgt cgtgggtttg agccagaaaa taggcagaat cagcaccacg gtaatggcaa
    14281 aaagggtggc gaaaatcgcc tgccaggaaa aatggaccag cagccagctt cctaacagag
    14341 gagccagggc cggagataga cccaccagcg gcatgatggt cgcaaaaatt cggttaactt
    14401 tctgtgaagg ataataatcc gtcactaacg cttgccagat aaccgccgcg gcgcagacac
    14461 ccacagcctg tacaaaacgc aataccagca gcatagcggc gttttctacc cacagcatcc
    14521 ccagactacc taacgcaaaa attgtcaggc cgattaataa taccggttta cgaccataac
    14581 gatcggagag cggcccccac agaagctggg ctgcggcaaa accggcaagg aacagactaa
    14641 ggctggcact gacagcagac gcaggcgttt gcaggtcggc ctgtatggcg gcgaaagcag
    14701 gcagatacat atcggttgcc agaaaaccga gtacgctcaa acccgccagc cagactaaaa
    14761 atcttttccc aggttgcatg tatattctct aaatctctca attaaacacc gccgcaaagt
    14821 gtagggagtg aaattagtgt tgtgaaacgg taatatttga tgactggttt caaaaatttt
    14881 gcaggcagac tatgtggtca gaatattcac tcgaagttgt tgatgcggta gcgcgtaatg
    14941 gtagttttag cgctgcggca caggagctgc atcgcgttcc ttctgcggtc agctataccg
    15001 tgcgtcagct ggaagagtgg ctggcggtgc cgctgtttga acgacggcac cgtgatgtgg
    15061 agctgaccgc tgccggagcg tggtttctca aagaagggcg ctctgttgtc aaaaaaatgc
    15121 agatcacccg ccagcaatgt cagcagatag cgaacggctg gcgcggtcag ttagctatcg
    15181 cagtggataa tattgtcagg ccagaacgta cacggcagat gatcgttgat ttttatcgcc
    15241 attttgatga tgtcgaactt cttgtctttc aggaagtgtt caacggtgtc tgggatgcgc
    15301 tttccgacgg gcgcgtggaa ctggcgattg gcgcaacacg cgcgattccg gtaggcggtc
    15361 gttatgcctt ccgggatatg gggatgctaa gctggagttg cgttgttgcc agccatcacc
    15421 cgctggcgtt gatggatggc ccgttcagcg atgatacgtt gcgcaactgg ccgtcgttgg
    15481 tgcgcgaaga cacctcgcga acgctaccta agcgtattac ctggttgctg gataaccaaa
    15541 aaagagttgt cgtgcctgac tgggaatcat cggcaacctg tatctcggca ggattatgca
    15601 tagggatggt gccaacacat ttcgccaaac cgtggcttaa tgaggggaag tgggtagcat
    15661 tagaactgga aaaccccttt ccggattcgg catgttgtct gacatggcag caaaatgata
    15721 tgtcgcctgc gttaacctgg ttgcttgagt atctcggcga tagtgagacg ctgaataagg
    15781 agtggctgcg ggagccggaa gagactcccg caacgggtga ttaacgacga tagtcgcgga
    15841 acgggccgtc agccacggag cggcgttcaa tcaagcgcgg atgcacttca atggactgcg
    15901 gttcttcacg tttgttgact atacgatcca acaacatgtt gaacgctgtt tcacccaacg
    15961 aatcttttgg ctgatggatt gttgtcagcg ccggcgtaaa atagcgggcg ttgcgcacgt
    16021 tatcataacc gatcagcgaa acatcctgcg ggacgcgcag ccccatttca tcagcagcac
    16081 aaagtgcgcc cattgccatg atatcgccac cacagaagac ggcagtaggg cgatgcggct
    16141 gcgacaggat ttgctgcatg gcgcgataac cggattcagg ttcaaagtca ccctgcacaa
    16201 tccagctttc cggcaccttg atcattgctt cttccatcgc cttcataaaa ccggcaaggc
    16261 ggcctgcgcc agtgttacgt tccagcgggc cggggataac gccgatttcg cggtgaccgc
    16321 gttcaatcag ataacgcccg gccatgtagc cgccttcgaa cgcgttatca atgaccgcat
    16381 cggtgaagtc agcttttgct tcaccccagt ccatcaccac cattgggata tggcgatact
    16441 cttccagcat cgccagcaac ggctctgggt actcagaaca catcaccagc agaccatcga
    16501 cgcgtttttg cgccatcatc gacagataag cccgctgttt ctcaagattg ttccacgcat
    16561 tacccagaat cagggtgtaa cctttctgga agcaattttt ttcaactgct tcaatgatct
    16621 cggcaaaata ggccgcttcg ctgctggtcg ctagcaaacc gatagacttg gtgtggttaa
    16681 ccttcaggct acgcgccacc gcgctagggg agtagtgtaa ttctttgatc gctgcccaca
    16741 cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt gatcacgtgt gacacagttg
    16801 tagtggaaac gtttgctcgt ttcgctacat cttttattgt tgccattcca tttcactcca
    16861 gaccctaact tcagctcctg caaaatcgca aggtaaacgt ttgcctttac acaccttacg
    16921 cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg agcgtcagga aggggtcaat
    16981 ggccagtacg caaatagtgt aagcgtggaa ttttggccga tcttgacgaa aaggggaagt
    17041 ggtaaaaaca cttatcatta taaatgacgg gagatttttt catcacagtg tgtaaaaatg
    17101 cgtaatatca ctttttgtgt ggtaataaaa aaggagaaaa cttgatgagc accgacctta
    17161 aattttcact ggtaacaacg attatcgtcc tcggtttgat cgtggccgtg ggtttgactg
    17221 ccgcgctgca ctgatttcct ctcgagggag cgcgttctcc ctcgcatctc cttcctgttt
    17281 gttagcaaaa tggcaaaagt gttttctctt tttgttattt tcattttttt gtgattgctg
    17341 tcatgctttc gccactttaa tgaatatcgc gtaatgacgc gaagaggtgg agataaggca
    17401 tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc gtttggtatc gggacaacgg
    17461 agttctcgcc aatgggcttg ttgcccgtca ttgcgcgcgg tgtggatgtc tcgattcccg
    17521 ctgccggaat gttaatcagt gcctatgcag ttggcgtaat ggttggcgcg ccgctgatga
    17581 cgcttctact ttctcatcgt gcccgccgca gtgcgttgat tttcctgatg gcaattttca
    17641 cgctcggcaa cgtactttcc gccatcgcgc cggattatat gaccctgatg ctttcacgca
    17701 ttttgaccag cctgaatcac ggagcatttt ttggtttggg ttcagtcgtg gccgcaagcg
    17761 tggtgccaaa acataaacag gccagcgcag ttgccactat gtttatgggg ttaaccctgg
    17821 caaatatcgg tggcgtgccg gcggcgacct ggttgggtga aaccatcggc tggcggatgt
    17881 catttctggc aacggcgggg ctgggagtga tttcaatggt aagtctgttc ttctcattac
    17941 ctaaaggtgg tgcaggggca cgacctgaag tgaaaaaaga gctggcggta ttaatgcgtc
    18001 cgcaggtgct gtctgcattg ctgacgacgg tactgggagc tggtgcaatg tttactctct
    18061 acacctatat ctctccggta ctgcaaagta ttacccacgc aacaccggtg ttcgtcacgg
    18121 caatgctggt gctgattggt gtcggattct ctatcggtaa ctatctcggc ggcaaactgg
    18181 cagatcgttc agttaacggc acgttgaaag gctttttgtt gctgctgatg gtgattatgc
    18241 tggcaatccc gttcctggcc cgcaataagt tcggcgcagc tattagcatg gcggtgtggg
    18301 gcgctgcaac ctttgcggtc gtaccgccgt tacagatgcg cgtgatgcgt gtcgccagtg
    18361 aagcgccagg tctgtcttca tcagtcaata ttggtgcctt taatcttgga aatgcgctgg
    18421 gagcagctgc tggtggtgcg gtaatttccg ctgggctggg atacagcttt gtgccggtga
    18481 tgggggcgat tgtcgcggga ctggcattat tgctggtgtt tatgtcagcc agaaaacaac
    18541 ctgaaacagt ttgcgttgct aacagctaat taaacaaaaa cgcagaagga taaccttctg
    18601 cgttacagtt gctgacaacg tgcgatttat cttgccgaat gcgctgttaa cgcctcatcc
    18661 gcgttggcat ggtaagcgaa gcgcatcagg caatactgca tttgccatca gttattatgc
    18721 agcgagattt ttcgctacga attcccagtt caccagcgcc cagaagtgct caagatagcc
    18781 aggacgtgca ttgcgatagt cgatgtaata tgcgtgttcc cagacatcaa cggttagcag
    18841 cggagtcgca tcggtggtca gcggagtacc cgcgttagag gttgaaacga tagccagttt
    18901 gccatcgctg tttttcacca gccaggtcca gccagaacca aagtttttga tcgctgcatc
    18961 agtaaactga gctttgaaat cggcaaagct gccaaaagat gcggcgatag cttcagcgac
    19021 ttttccagtc ggttcgccac cggcgttcgg tgccaggcag ttccagtaga aagtatggtt
    19081 ccagacctga gctgcgttgt tgaatacgcc accttcagag ctgcgaataa tctcttccag
    19141 tgatttacct tcaaacgcgg tacctttaat caggttgttc aggttagtga cataagtctg
    19201 atgatgcttg ccgtagtgat actcgatagt ttccgcagaa atgtgcggtg ccagagcatc
    19261 tttagcatat ggtagtgcag gtaattcgaa tgacattgct actctccttt attattaatt
    19321 tgcatacgta caatagcctt attgtgcgta tggatagggt agcaaattaa aaggtgagac
    19381 aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa gttttagcga agtgtttttg
    19441 gggtcattac ccggcgagcg ccaacatagt ggcgctgcca gtagtcttca ctgagagaag
    19501 tgatttgaat ttcctgacct gtgcgcggtg actgaataaa tttgccgttg ccgacataca
    19561 cgccgacatg atcggctgta ccgcgtccct gagtacggaa aaagaccagg tcgccgtttt
    19621 tcagttcact acgttcgatt ggcgctgcat cgcgcaggtg atacatttca ttcgccgtac
    19681 gcggaatacg aattttcacc aaatctttat aagcgtaata aaccaggccg ctgcaatcaa
    19741 aaccggtacg cggtgagctg ccaccccaac gatatggctt accaatttgc tgcatcagtt
    19801 tattcattgc cacttttgtg gctttttgca ccttcgcttt gtgtgcgtcg gcaagggttc
    19861 ctgacgctgc atttttgact ttcacacaat gcgatttata gcccttacgt ttggtacact
    19921 tttcggtaaa gctggctgag gccgttttat tgacggtatg tttgctgcga gttttggcgt
    19981 ttttagaagc ggtaatggaa gattttttaa cggaagaagc ggttttgctt ttggtcgtac
    20041 ttttactggc ggcttttttc gctgttttct gggtcttttt ggtggttgcc gtgcttttct
    20101 ttttatctgc ttttttggtg atatgagtgg tagcagaact ctccctggct tgctttgaag
    20161 catgggccat aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa
    20221 tacggtttat ccgcgccact gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc
    20281 tgatttacaa aacttgatga ttctaatgca tagaagaaga gaaagttaat agcttttatt
    20341 gttaaaaaat acgtttcgtt ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg
    20401 tcataaatac gccagtttgc cgtgcatcag ggaagaaacg aatatttgaa atgcaacaaa
    20461 tagagcaaag ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact
    20521 gacccgctac aatgtcagat tactgccgta aagaagtttg aggaagcaag acaatgagca
    20581 ccactatcga aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg tacatgaaag
    20641 gttcaccgaa actgccgagc tgcggtttct ctgcccaggc agtccaggcg cttgccgcat
    20701 gtggcgaacg ttttgcctat gttgatattc tgcagaatcc ggacattcgt gcggagctgc
    20761 cgaaatatgc taactggccg accttcccgc aactgtgggt tgacggcgag ctggtaggcg
    20821 gttgtgatat cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg atcaaagaaa
    20881 ctgccgctaa atacaagtct gaagagccgg acgcggaata atccgtcctg caatactgac
    20941 ggatgcgaac gggcatccgt cagtcctttc tctatcccca atccagccct tgtggcgaac
    21001 ttgaaaatcc cgcttcacgc agcagcgtaa acatcggcgt ttgccagacc ggtagatcat
    21061 tcacttctgt tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggta gtcagcgcgt
    21121 ggaaaacctc tggggcgagt aattcctctt tttcctgcca gaccagcatt tttttgccac
    21181 cttgcgccag atagagtaac aatttgccgc cagaaacgac caccagcgca cccgcccgac
    21241 gcgttggtac cagcgttgcc ggatgtgcag gccagggcag aagatttccc cacacattag
    21301 ccggatcgtt ggcggagagc gccactggtg tatagtggcg cgtttgcgcg gcttgtgtcg
    21361 ccaggtcgcg caagcggtca atagtcagac gttcagcgaa ttgcgcgcca cccagacctt
    21421 ctacaaaacg acctcgcata attcgcccgg aatcttccat acttcgacaa agcgtttgca
    21481 tcgatggaaa cccgccaggg atattttcgg ctatcaccgc ctgacgactg atgatgccgt
    21541 agcggtcgag catattttcc gccagcgcca gcatcctttc ggtatcgttt agtggctcca
    21601 cctgcaataa cgaccagcgt ccagccagat ttggtgtgtt gtaagatacc cgcggcgaga
    21661 cgggttgtgc atagacagga cgcccacgac gagcccggtg actgcggcga gttgaggtgc
    21721 gtgcgttgga actgctgcgg gtgagggcgc gtaacggtgc ccaaatatcg ctggtgatga
    21781 cgccttgcca gaccagcgcc cataacgcct cttgcagtgc cgagggatca accgattcgc
    21841 cgattttgtc gcgtatccgt tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca
    21901 gaacagcgac tatcgcttgt tgcagcgccg aacgattcgc ctgatccgct tcggcgggag
    21961 tgaacgattc tgcagcatat tcctgtagat gcagtgccac caggccgtca tcttcaccca
    22021 gttttttttg ccccgaccag ataaccgcac cggttgccag taattcatcg agcacttctg
    22081 gcgaatagtc gtgtacgcgg gcaggcaaga tctggctttc ccagagtgag gcgggtaagc
    22141 cgactccggc aagctgttcg atcacccgca tcacgccatc caccccctca taaacgcctg
    22201 gcgatgttga ggcaaagagc gccgggctac catcggtggc gggtaatacg ccctgacgtt
    22261 ccagcagcaa tcgcgcatag gtcgtggctg caacgggacg cgtcgcttct ctggcggctt
    22321 gcagcgagcg caaacgcaga cgacgaaata cttcatcgct cacccagatg tcttgttgca
    22381 gattcatcac cagaccctgt tcgcgcagtt gctgaagctg ctcttcgaca atggcaatac
    22441 cgagactaaa ctcatgagcc agttgttcag ccgtgaccag agcatgagcc cggagatagc
    22501 gtagaaagag gtcgcgaagc gggtaactga ctctatgcaa ataaatctct ggcagtgact
    22561 ctggtaatcg tacgccgagg gcatcgcgca gcctggcggc atcatccata caggcaagac
    22621 gctcctgtcc actaatcatc gtcgggaaga tgcgttttac tgtaagaaga ttttccagat
    22681 acgaggcaat ctcttcactg ctgcctgtat gccgttgcgc caggtcttca acggtcattg
    22741 gccccagttc gcgcagcagg tcgaacaatc cttcttcacc tttcgctctt ctgccaggag
    22801 ccagtcgttg caactcttct tccacctggc gaatgacctg cgggtcgagt aattcccccg
    22861 gatcgacctg tccgagtaga ttgcgcagta actcgctgtc cagcgacagt acggatgccc
    22921 ggcgttctgc cagcggggcg tcgctctggt acataaattc cgcgacatag ccaaacaata
    22981 aacttgtggc gaaaggcgag ggcgtagtag tcgttacatc ggatatttga atttcgcctc
    23041 cgttcaggcg acgcatcaaa cgttccagtg cgggaagatc ataaacatct tgcagacatt
    23101 cgcgtagggt ttcgagaatg gccggaaaat ccggatatcc ctgagcgatt tccagcaact
    23161 gactggcgcg cagccgttgt tgccaaagcg gggtgcgatg gcccggcgtg cggcccggca
    23221 ttaataatgc ccgcgcggcg cattcgcgaa aacgggcggc gaaaagtgcc gaactgccta
    23281 ccgcctcgcg gacaatttgc agcaactttt ctggttcaaa caaaaaaatc gcggcgtcgg
    23341 gcaatttacc atcagtgtca ggaatgcgcg caacaatgcc gtcatcactg gcgaccacag
    23401 acgcgtcagc gccccaaagc gcatgtattc gcccggcaat cgccagcgcc cagggttcat
    23461 gcacccgtct tccataggga gagtgcaaaa taatacgcca atcgccaatt tcatcacggc
    23521 accgttcgag gaccagatga cgactccccg gaacaatacc cgtcgcgttg cgctgctcgt
    23581 caatcaaccc ctgaatattg gcgatcgtat tttcttcagc cagccacggg ggaatagtcc
    23641 cggaaaagaa cgctccatcc gccagcaaat gaagaaaatc gccgatcatc tcgcctaatt
    23701 cagccggacg tccgttacct tcaccacgcc agaaggggag ccgggcagaa cgacccggag
    23761 caggagtcac aatcacctga tcgcgggtga tttgctggat ccgccatgag gttgcgccga
    23821 gagtgataat atcgttcacc cgcgactcat ataccatctc ctcatccagt tcacccaccc
    23881 gtcgcgaacc ggcctgttct tccccttcgg gtaataacac gctatacatg ccacgatccg
    23941 gaatggtgcc gccgctggta accgccaaca gctgagcgcc aggtcgggcg gtcaatatcc
    24001 cggtctccct gttccagacc agtttggggc gaaaagcaga aaaatcgcca gagggatagc
    24061 gtccggaaag catatccagc gtggcgtcaa aaacacgtct tggcagatct ttccacggtg
    24121 cggcacggcg tacgcgggag taccattcgt ctacctgtaa tgcatccatc gccgcggcgg
    24181 caacggtgtg ctgcgcaagg acgtcgagtg gattatgcgg aggtgtcagg ttttccagcc
    24241 tgcctgcgaa catacactct acaatgactg cggaatcgac taagtcacgc ctggttcggg
    24301 ggaaaaaaag ccctttggag atacctccta tctggtgtcc ggcacgtcca atgcgttgta
    24361 acccactggc aacagaaagc ggcgttgcca cctgaatcac cagctccaca gcgcccatat
    24421 caatccccag ttcgagactg gaagttgcga ccacgcagcg taactcaccc gatttcagcg
    24481 cctgctcggt gattgctcgt tgttctttag agacggagcc gtggtgtgag cgggcaataa
    24541 aaacgtcgct actttgtaca cggttagagg ttgcgccaga ggtcgactcg aaatgggccg
    24601 catcagcggc gatagacggg gaacgctgta agcgtgcggc gtaaagctca tttaatcgtg
    24661 ccgtcagttt ttccgccagc ccccgcgaat tagtaaagac aatggtcgag cgatggcgca
    24721 acacttcatc aaggataccc gtttcaatat atggccagat ggagccttcc cggccggcat
    24781 ggctgtcttc gccagtgccg ctggcgaccg atgagacatc atccatattg gcgactggta
    24841 cgacaatccg tatctgcgga tgacgcattg cgggcgggtt gactaccgta accgggcgat
    24901 cgccaccaag aaatgctgcc acatcgctgg ctgagcgcac agtggcagaa aggccaattc
    24961 gctgtgctga ggtgtggagc agcgcatcga gccgctccag acttaacgcc agatgcgcac
    25021 cacgtttact tcccgctacc gcgtggactt catcaataat taccgtttcg acgccgcgta
    25081 gcgtttcgcg cgcgcgggag gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa
    25141 tatccggcgg attacgggtg agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga
    25201 tccccacgcg aagattgact tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct
    25261 tcaacgggat ctgcaagttg cgctgaacgt cggtgcccag ggcttttatc ggtgaaatat
    25321 agaggatgcg tgaggtttta cgtttatgcg cctcgcgggt atcttcgccg ccttcgcgga
    25381 agagtcgatc gagggcgtag agaaatgctg ccagcgtttt cccggagcca gtcggtgcaa
    25441 tcaccagcgc atgttcgcct cgcgccgcca catgccaggt ttgcgactgg acagcggtcg
    25501 gctgtttaaa ggcgcgaaga aaccagtcgc gggtcgccgg tgaaaacacg tccggcaggg
    25561 gcgatgaagg gtttggatta tctgccatta acgctattcc ccgtgaatat catgcatgat
    25621 gcgtggaata gcctcactat agatgcctga atcattgcat gtcatcaggc atcgactcga
    25681 ttacacctct tcggcggcag gtagcggcca gcctcccaga cgtttccagc ggttgacgat
    25741 ttcacaaaac agcaccgcag tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct
    25801 gtcgaagtcc atgccagcgg tctggcaagc ctttgacaat acggtttgtc cgagtgccag
    25861 cccggccagt gcagcagtgt caaaagtggc gaaagggtgg aacgggttac gtttcagtga
    25921 ggcgcgttct gcggcggcca tcataaagct gtgatcgaaa ttggcgttgt gcgccaccat
    25981 aatggcgcgg ttacagccgc tcgctttaat acctttacgt acaactttaa aaatttcgtg
    26041 cagcgcctcg tattcgctga ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa
    26101 agcaagggct tctggttgca aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt
    26161 ggtgtccggc atcagccagc cttgttcatc cattttcagg gtgatggcgg caatctcaag
    26221 cagcgcatcg gttttggcgt taaatccagc tgtttcaaca tcgatcacaa caggataaaa
    26281 gccacgaaaa cggtcgcaca gaccggtaag ttgagcgtta tcggacatct gattctctta
    26341 atacggataa attgcagcgc gcattatgac aaatattact tgtcgatgca gtaaagatgc
    26401 gggcgcgatg agttcgcgcc cggcaggaga ttagttgccc agaccgcgac cggcgtcttt
    26461 ctcttcgatt aactcaattt tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt
    26521 accgcctttt accggacccg cttcacgggt cacgttaccc ccgttttgac ggattttttc
    26581 gcacgcttca gcggcattat ctacgctaag cgcgatgtga ccataagcag tgccgagttc
    26641 gtatttatcc acgccccagt tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc
    26701 gtagccaaca aacgccagtg agtatttgta ttccgggttt tcgctggtac gcagcagttt
    26761 catgcccagc actttggtat aaaaatcgat ggagcgttgc agatcgccaa cgcgcagcat
    26821 ggtatgaaga agacgcatct ttttatcctc aaaatggcta acagattcaa aatgttgttt
    26881 tagtatagcg gcggctttac gccgctcgca atgttggatt acaacgtcgg gtaatcagtg
    26941 tagccttccg caccgccacc atagaaactt tcagcacgct gtgggttaag ctcagctttg
    27001 cgctgcaagc gggcgaccag atccgggttc gcaatccagt cacgaccaaa tgccaccgca
    27061 tcaattaacc ctttgccgat cagcgtttca gctttttcta ctgtgtatgc acctgcgccg
    27121 ataatcggac cgtggaaacg ggcgcgtact ttttcgcgga acgcatcagt atacggttca
    27181 ccccccgccc aatctggttc tgacatatgc agataagcaa tgccgcgttt acccagttgt
    27241 tcaatcagat acagtgcatc ggcttcttca ttcgggccgt tatccgtgtt ctggaaagta
    27301 ccgattggcg aaacgcgaat gccaatgcga tcggcacccc attcttcaat cccggcatcg
    27361 accacttcca gtaccaaacg tgcgcgattt tccacgctgc cgccgtactg atcggtacga
    27421 tggtttgaag aaggagaaag gaactgatgc agcaaataac catgagcaga gtggagctct
    27481 accagatcaa aaccggcttc acgcgcgtta gcaatagcct gacggaaatc attgacgata
    27541 cttggaatct cttccagttc aagcgcacgc ggcatggatg tttcaacacg gatcgcctga
    27601 ccattttcat cgcgcagaga agtacgtgtt cccgcgctaa gtgctgaagg cgctactggt
    27661 gcctggccgc caggttgcag gctggcgtga gaaatgcgtc cggtgtgcca cagctgtacg
    27721 gccatatgcc cattttcagc atgaacgcca gcggtaattt ttttccatgc ggcaatttgt
    27781 tccggactat ggatgccagg tgcaccagca tacccttttg cctgggcaga aatttgcgtg
    27841 gcttcgctaa taatcaaacc ggcactggca cgttggcgat agtattccgc catcaacggg
    27901 gtagggatgt cacccggttc aatactgcgc agacgcgtca gcggtgccat aaaaatacgg
    27961 tttgccgcag ttattgcgcc cactttcagt ggggaataca gtttttcaga tgacataaag
    28021 gctcccggtc gtctggtgat aaaaaatgcc tgctaaacgg caggcgtcgc aataatggtt
    28081 tttacatgtg ccagcgcgtt ttccagtggc tcggaattgc gcgaaatttt ggcctgcaga
    28141 ttcgcaccca gccatagtgc gtaaagcact tgtgcctgtt gcagcggttc gccacaaaag
    28201 gttaaacaat ggctatcacg gccattttcc agagcctgcg agagcagggc gatcacgccg
    28261 cgagcgcctt tatccatcgc gctacgcata tcttctgaca gatcgcacac ttcggcagag
    28321 agttttactg tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagtgtttgc
    28381 tggtaataag ccagtatgcg gtcgcggtag ttaccttcgc cggattgcag caactcagtc
    28441 agtcgttggt gatatgtggc gtaatggcgc tcaagcatgg caacgccaaa cgcttcttta
    28501 gagcgaaagt agtgatagaa ggaccctttc ggcacttcag cggtttttag taattcgctt
    28561 agccccatcc cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt
    28621 tcgcgagtat catgttcggt gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc
    28681 tagtgcaagc aaagttgcta ctttgggcat taacagcttc aataatggta ataatcttat
    28741 ttgaggaggg cgaagtggcg gagcaattag agttctttcc cgtccagagc ccgtgccggg
    28801 gaatttgcca gtctgacgaa cgcgggtttt gtcgcggctg ttttcgaagc cgggatgagc
    28861 gttttaactg gaataaaatg agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc
    28921 agcgattaat gcgcaaatta cgtgcaaata agccagcgtc atcagacgaa ccggagcaac
    28981 catcactctt ttgagcctaa tcttgcgtat actcctctca tatctgataa gaggaagcga
    29041 ttatggttca gcgtattact attgcgccgc aaggcccgga gttttcccgt tttgtgatgg
    29101 gctactggcg attgatggac tggaacatgt ccgcccgcca gctggtcagt tttattgaag
    29161 agcatctgga tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt
    29221 gcgaagcggc gtttggcgag gcgctgaaac tggcacctca cctgcgtgag cggatggaaa
    29281 tcgtcagtaa atgcggtatc gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca
    29341 tcactgaccg cgatcacatc attaagagcg ccgaacagtc gctaattaat cttgcgaccg
    29401 atcatctgga tctgctgtta atccaccgcc cggacccgtt aatggatgcc gatgaagtgg
    29461 cggacgcgtt caaacatctg catcagagcg gcaaagtgcg tcattttggc gtatccaact
    29521 tcacgcctgc gcaatttgcc ctgttgcaat cacgtctgcc gtttaccctt gccactaatc
    29581 aggtggaaat atccccggta catcagccgt tactgctgga tggcacgctc gaccaactgc
    29641 aacaactgcg tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg
    29701 atgattattt ccagccgctg cgtgatgaac tggctgtggt ggcggaggag ttaaacgcgg
    29761 gctcgattga acaggtggtt tacgcctggg tattacgttt accgtcgcag ccgctgccaa
    29821 ttatcggctg aggtaaaatt gagcgcgtac gggcagctgt cgaagcagaa acactgaaaa
    29881 tgacccgtca acaatggttt cgtatccgta aagcggcact ggggtacgac gtaccgtaag
    29941 cacttttagg aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg
    30001 cacaatgaac ggaggtccta tgaaacgttt tagtctggcc attctggcgc tggttgttgc
    30061 gaccggcgca caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt
    30121 tgggcagtca attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc
    30181 cgatctgaaa gcattacctc ccggtgaaca tggcttccat attcatgcca aaggaagctg
    30241 ccagccagcc accaaagatg gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga
    30301 tccacaaaat accggtaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc
    30361 tgcactggtc gtcaataatg acggcaaagc taccgatgcc gtcatcgcgc ctcgtctgaa
    30421 atcactggat gaaatcaaag acaaagcgct gatggtccac gttggcggcg ataatatgtc
    30481 cgatcaacct aaaccgctgg gcggtggcgg tgaacgctat gcctgtggtg taattaagta
    30541 attaagaggc cagcgtacct tgcggtggtg cttgctcaag ttgcgaaagc gagcagtaca
    30601 gccgccagac aattgccgcc agctcacggg cggcaggttg atgatggcgg gcaaggctgt
    30661 cgcaaatccg ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct
    30721 cactcatcac tccccgcaac agtgaaatac agttatcccg tactcgcgat aacggatcgg
    30781 aacgcgattc ccagtcgcgt aattgccaga caacatgaga acagttcagc agcactatac
    30841 cccagcgtaa taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt
    30901 gatgataagt gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagttgat
    30961 cgacaaaatc ccggcgcagc gcgcgaatat ggcggcggct tttgcgagca tccgatcctg
    31021 gacgcagaat ggcgaacgct aaccacgcca acgcgacgcc aacgattttt gccagattat
    31081 cgttaagaaa atcagcaaaa ttatacaccg ggggattagt gacggcgata aaagaaccca
    31141 taaaaacaat cagttgcccc cacaatgcgg caaatttggg catctgcaat ttaagaagct
    31201 gcattgtcgc cagcagtgga aagagaaaca gtaaaaattg ccacagatcg ctaatctgga
    31261 ccatcagacc aaatttgacc acaaagctga atagcgaaag taacaccagc gtgcgcatca
    31321 gcagtgacaa cgacttaaac ggtgctgcga cggcggagta gagtacgcag ctaatcgctg
    31381 ccagcgttaa tgcattggca ccggcatccc attgcgaagc aatactccat gcgccaatca
    31441 tcatcaacgt acaaaatgta cgcagtccgc tccacatagc ttcggcgtta tcggtatgac
    31501 gagccaggcc ggatgtccgt gggagtctgg catgatcatc tacatcgctt tgcagacgat
    31561 gtaattcctg actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg
    31621 cgacgtgccg atagtcggcg acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg
    31681 tgtaaacatc tgtttgcgaa ctggcgagcg ccgtcagcaa ctgttcgaga atttctcgcg
    31741 tggcacctgg cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg
    31801 tcatacgtaa ttgctggtgg agcagcgcat taaggcgcgc gttttgctgg cgaaaacgat
    31861 agtggctcca gaaagcctgg atacgcagca aattcatggt cagtatctgc ccaatcaccc
    31921 cttcatgtgc tgcacgaatg gcatcggttg tttcaggctg ccagagtaaa ctggcatgtt
    31981 ccagtaatcg ggcgtgcatg tttttcaatg cggttaaaag ggctgtagca tcggaactgc
    32041 taggcaggat catcatcatc atgccgccgc acaaaatacc gacaatcacc tcgcaaacgc
    32101 gcgcctgagc gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga
    32161 tgatggcagc cgtgtagccc gccagttgaa atgcatacgc gacgttattc gtgaagtgcg
    32221 cacaggccca ggtacaaaag ccaagccacg ccgacatgct caatagaaaa aaccacggct
    32281 cattgagcgt atgcccggca agaagcagtg ccgcaatggc tcccagtaaa ctgccagcga
    32341 tgcgtccgag gcttttgctg atgacaccgc caacggtggg aaagctaacc actgcagccg
    32401 aggtcatcgc ccaatagggt tcatccagat tcaaataata ggcaaccgtc agcgccagac
    32461 acatggcaat ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ctgaaccagg
    32521 gcaaattgcg caaggaccat gacgatgcgt tcattattga cctacggcaa tgctacaggt
    32581 ggtgcccgac accagcgtga catcgcctgg aactttatcc agcgcaaagc ggacgggaac
    32641 gcgttgggcg agacgaaccc aggggacgtt aggtttcaca tccggaataa ggcttgagtc
    32701 actttcaacg ctctgatcat aaatcgcccg accgatactg gaaacgtgac cctgtaacgt
    32761 tttgttatca ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt
    32821 ggtttcttca aaataaccaa tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg
    32881 tttcccggtg tcagcgtaat cgccgatgcg ggtagtgaga ttagtcaccc atccactcac
    32941 cggggcgcga atttccgttt gcgccagttg ccattgtgtc tgtttcagcg tcgcctgtgc
    33001 ggcatcaaca ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc
    33061 agaaataaaa ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag
    33121 gtcagattgc gctttcgcca gctgagcctg tgcattcagc tcggcgatct gaaagggcgt
    33181 tttatcgatc gtgagcagaa gatcccccgc gttaaccagc tggttgtctt ttatattcag
    33241 ctcaacaata cgtcccgaca cctgtggggt gatagaaacc tgttctgcgc gtattttccc
    33301 gtcgcgtgtc cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc
    33361 aactaccgct acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac
    33421 gcaatcagta ttgccagagc aaggcaaacg caaatcgcaa acagcgataa atccattaac
    33481 aagggatgcc agatgtcacc ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc
    33541 cagatgacga atccaaacgc gaaggctttg aaaaacggag gaaagtagac ggacgcaccg
    33601 aacaccagat cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt
    33661 cctttacgac cgaatatatt gcgttagatt aataaatatt ctttaactgc gaaaaattta
    33721 cgcgcaattt ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag
    33781 caatacaatt gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa
    33841 ggagatgaaa ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg
    33901 tgctctgata gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt
    33961 acacaatatc catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg
    34021 catcgagcag ccatcactgg tccgtactct ggaccaactg gaagaaaaag ggttaatttc
    34081 gcgtcaaact tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga
    34141 gccgctgatc agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa ttttacatgg
    34201 catctccgca gaggaactgg aacaactgat tacgctcatc gcaaaacttg agcataatat
    34261 cattgagtta caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca
    34321 cgcaaactta tttagcgcgg agaaacggtc acctgactgc cattgctggc cagtacgaca
    34381 cgttggcccg gagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca
    34441 tcgtctttac gaatttccag ctcgacacct tgcgttttgt tcattgcact ctgtacgccc
    34501 tgaccagcta cgccacctgc aacagcgcct gctgcagtag ccagagaacg cccggttccg
    34561 ccaccaacag tatttcccag gaaaccacca agaacagcac cgccaattgc accgataacg
    34621 ttggaatcat caccgccctg aatctgtacc ggacgtacgt taacgatggt tccatagctg
    34681 acattctgta cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta
    34741 ttaacacaac cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt
    34801 gaaaaatctc ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg
    34861 gcatatgtga atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag
    34921 gatcaaccaa ttataaacaa aacattcatg atgattattg atttgaagat aatgttttac
    34981 taccaacggt aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgt
    35041 gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat
    35101 gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg acaattgatg aacaccgggt
    35161 cgcacagctg gcaagtttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga
    35221 tatttgccag ggccagcagc ttacactttc gcagtttgga cagcttgata ctcaactcgg
    35281 gcgacttttt gctgatgcgg tcaaggcctt gcttaaggaa caaaacctgc aggcgagaga
    35341 tatagttgcg atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc
    35401 acacacttta cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt
    35461 cggtgatttt cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc
    35521 gttccatcat gccttgctgg ctcacccaac cgagcgacga atggtgctca atattggcgg
    35581 catcgccaat ctgtcactgc tcattcctgg gcagccggtt ggcggctacg ataccggtcc
    35641 tggtaacatg ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt acgataaaga
    35701 tgccgagtgg gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga
    35761 cccatatttc tcgcaacctg caccgaaaag caccggacgc gaatacttta actacggctg
    35821 gctggagcgc catttacgcc attttccggg tgttgatccc cgtgatgttc aggccacact
    35881 ggcagaactc accgccgtga ccatttctga acaagttttg ttgagcggtg gctgcgaacg
    35941 attgatggta tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc tggcggcatt
    36001 actgccaggc acagaagtca ccaccaccga tgccgttggc attagtggcg atgacatgga
    36061 agcattggct ttcgcttggc ttgcctggcg gacgctggcg ggattaccag gaaatctgcc
    36121 ttccgtcact ggcgcaagcc aggagacggt tctgggggct attttccccg ccaactcgtg
    36181 atatcagaat taactgaatt ttttcttatc gtgtctgccg ttacacttgg aagattaagg
    36241 aggatatttg ccctccggac caggaaagtc ttcgggatat gactatgaaa aaactgttaa
    36301 ttatcatttt gccggtcctt ctctccggct gtagcgcctt taatcaactg gttgagcgaa
    36361 tgcaaaccga tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata
    36421 atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggcc
    36481 tttcagcctc tggcgcgcgt tactccgacg ggatctatgt tttctggtcg aaaggtgagg
    36541 aagcgactgt ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta caaaatccac
    36601 agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccacagaca atccgtaaag
    36661 aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc
    36721 aaaggcgggt tacgccgccg cgatcttccc gccgatccat taaccctttt tgaacgttgg
    36781 ctctctcagg cttgtgaagc caaactggcg gaccctaccg cgatggtggt cgctaccgtg
    36841 gatgaacatg gtcagcctta tcagcgcatc gttttactca aacattacga cgaaaaaggc
    36901 atggtgtttt acaccaacct cggtagccgt aaagcacatc aaattgaaaa taatccgcgc
    36961 gttagcctgc tgttcccgtg gcataccctt gaacgccagg tgatggtgat cggtaaagca
    37021 gaacgacttt cgactctcga agtgatgaaa tattttcata gccgcccgcg tgatagccag
    37081 attggttcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt
    37141 aaattcctgg agctgaagca gaagtttcaa caaggcgaag tgccattgcc gagcttttgg
    37201 ggcggttttc gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg
    37261 catgaccgct ttttgtacca gcgtgaaaat gatgcgtgga agattgatcg tcttgcaccc
    37321 tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct
    37381 atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca
    37441 tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag
    37501 cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct
    37561 attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat
    37621 gcctgaaacg cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg
    37681 gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg
    37741 ttcaggagtg ggtggacaaa atccgcaagc aggttgcccc gttcctcgat ttcgactgtg
    37801 gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga
    37861 ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg
    37921 ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc
    37981 tgttgcaggg ttatgacttc gcctgtctga acaaacagta cggtgtggtg ctgcaaattg
    38041 gtggttctga tcagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc
    38101 agaatcaggt gtttggcctg accgttccgc tgatcaccaa agcagatggc accaaatttg
    38161 gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct
    38221 accagttctg gatcaacact gcggatgccg acgtttaccg cttcctgaag ttcttcacct
    38281 ttatgagcat tgaagagatc aacgccctgg aagaagaaga taaaaacagc ggtaaagcac
    38341 cgcgcgccca gtatgtactg gcggagcagg tgactcgtct ggttcacggt gaagaaggtt
    38401 tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg
    38461 aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg
    38521 cagacctgat gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta
    38581 aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact
    38641 tctttaaaga agaagatcgt ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga
    38701 attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact
    38761 ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt
    38821 gtttatggtc atgcgggtaa cagtgcggca gagtttccga tgcgccgcct gggcgcgaac
    38881 gtctggccgc tgaacaccgt tcaattttct aatcacaccc aatacggcaa atggactggc
    38941 tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag gcattgccgc cattgataaa
    39001 ttacacacct gtgatgccgt attaagtggc tatctgggat cggcggagca gggtgaacat
    39061 atcctcggta tcgtccgtca ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat
    39121 ccggtaatgg gtcatccaga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat
    39181 gtgcggcacg gtttgcctgc cagcgatatc attgcgccga atcttgttga gctggaaata
    39241 ctctgtgagc atccggtaaa taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt
    39301 gcgcaagggc cacaaattgt attggttaaa cacctggcgc gagctggcta cagccgtgac
    39361 cgttttgaaa tgttgctggt caccgccgat gaagcctggc atatcagccg tccactggtg
    39421 gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg
    39481 aaactgcttc agggggcaac gctgcaggag gccctggaac atgtgaccgc tgcagtctac
    39541 gaaatcatgg tgaccaccaa agcaatgcag gaatatgagt tgcaagtggt tgctgctcag
    39601 gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa tatctcattt
    39661 cggcccacaa cgaactgtgg gccgacggct attactttaa gccttccgct gacagcgcgt
    39721 cttgtacttc cggacgttca gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt
    39781 ccagattcag tttcaccgcg tatgcccagc gcagtaccgt aaacagatag gcatcagcaa
    39841 ttgtaaatct ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca
    39901 gtttcttctc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta tcagggcgaa
    39961 acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt
    40021 tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa
    40081 gatactgcat aatcgctaca ccttccgtca gcaaagtacc gtcatccagc agcaatgcag
    40141 gtacctgtcc cttagggtta acggcaaagt aatcgtcacc gttttcgaga cgttttttca
    40201 ttaaatccac actgacgagc gtaaaatcct ttccgctctc acgcagggtg atatgggaag
    40261 cgagagagca ggcgcccggt ttgtagaaca atttcatcgg taactccttt tgtctgtggt
    40321 ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct
    40381 tgtaaaagag tttccctgaa ttttacgcta cggctgcttt cgccgctttg tctgcagcgt
    40441 catcctgcgt catgcggtgc agtttcggcg cggtcagcag catcagtact gcaataacgg
    40501 cagtagcgac accaatctgc aagaatacgc gaccatagac ttccagtgac atcagcggat
    40561 cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac
    40621 cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt
    40681 gtgcaaccat tgccagaccc agaccagaga tcatcagttc cccgatactc tgcaggccat
    40741 agctcgcgac cagccagctt acagacacga tacctgcgtc agacgcgaat ttcgcaccca
    40801 gcggcagaat caggaacgca ccagaacaca tcaccatacc gattgcaaac ttggttggca
    40861 tcggcagggt atcgcccatc ttgttataga tagcggccag aatcgggcta ccaatgatga
    40921 tccagaacgg gttcagtgcc tgatactgct caggttctac ggccaggccc agaatggtat
    40981 gttcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga
    41041 agaagataat ggcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac
    41101 ctttcatcgc gaaggcttct ttaccgaaga taaccacgat accgaaggca acaacgccca
    41161 gcgccatacg cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg atcagtgcca
    41221 caacaccaat aatggtcagc agcaggttac ggtagttgat aggctcgaag tctggttttg
    41281 aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc
    41341 ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggggtgg
    41401 cgatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt
    41461 ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa
    41521 acaggccgtt accgaccgca atagccgcca tacccatata aacgataccg gcgtcgtgac
    41581 cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc ataattacgc
    41641 gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca
    41701 gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa
    41761 ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa
    41821 tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag
    41881 ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg
    41941 gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac
    42001 ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat
    42061 ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa
    42121 aaaacgtaac ggcagatttt ttcactgcat tgagaggggg atgatggctt gacggcgata
    42181 tgttctgcta tttagcgttt ttcctagtga gatagtccag tttcttaaaa atagccagtg
    42241 taatgttttg caggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctgattc
    42301 ttgcaatgtt tttacattgc cacagcatcg tgatcttgat cactaatgaa tagaaaattt
    42361 cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat tacctgatga
    42421 taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg ataaagaaag
    42481 gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt ctctttgtat
    42541 tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat gcaggtataa
    42601 cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg aaccactttc
    42661 agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt acgattacaa
    42721 acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt gtttaatacg
    42781 acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg atcttccgga
    42841 acctcgctat tatgctgctc cagtaagata cggcaggttt tgatgatatt ttctgctttg
    42901 ctgttataaa gcccaatcgt tttgatatag gttttcaccc cttcaacgcc cagttcaagc
    42961 atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt aacactgaca
    43021 tcggttgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga actgaaatta
    43081 agctcagtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc caggcgtttt
    43141 gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt
    43201 cagtacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc atcagtccca
    43261 ggccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa
    43321 tctccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg tcaaacaatg
    43381 tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag gttgcgccca
    43441 taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggca gcgaaggctt
    43501 cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc cctaatgatt
    43561 gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc accgaggcga
    43621 tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa
    43681 tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc agagcgttag
    43741 tggcagtaga cgtgacagcc aacagaggac aaaggccgag caactggacc agcgcagagt
    43801 tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt tattctccac
    43861 aggcaggaag ttgagaaagt tgttccggta acgtctgagc gtacaatccg gcgcgtttta
    43921 ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg aaattaccac
    43981 catctttctt caccgcccag tgcgcatcat ccgcaccact gattttttta cccgcaaaat
    44041 gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc gtttcgtggt
    44101 gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa
    44161 tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc ggtttgtcat
    44221 cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca gttaccagat
    44281 agcaactctg tgccagcgca ttgttatagc gttcggctgg cagcacctga tcaaataaag
    44341 ccttttgttg cagactggcc tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg
    44401 cagttaaccc tgttgaaccc gctgcaaata gcgccaacgt aatgccgtgt tttcggatag
    44461 ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga
    44521 tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca ggatagccgc
    44581 cgaaactgcg gatcaaccag actaataagc ccgcaagcgc gccgaaaata agacgaccac
    44641 gattggtcgt agaagcggta accgggtcag tcaaaataaa gaatgcgccg agcatggtcg
    44701 ctccagacag cagatgaatt tgcggtgctg ccagtgtttc tggtgagaac aaccagccca
    44761 acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag cgaatcgctt
    44821 tctgccatag caaccatacg ccgccagcca gccaggcgag atttacccat tgccaaccag
    44881 cgcccgccag aataccgctg tagatcggat attgcataat ctgttcaacc gaatgaccgg
    44941 cacggacaga ggttttaaat gtatccagcg gtgtcgcctg actaatgcca tcaataccta
    45001 agcgtagtgt gttcatatca ccaccactgg cggtatgccc gctgaaaata acctgtatgg
    45061 cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct
    45121 gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta aacgggtttt
    45181 gccccagacc gccatacaac tgtttagcga tgataaccgc aaacaccgta cccagcacga
    45241 ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct gtcagcaatg
    45301 ctgagttatc tttcaacgtg gcggctaccg actgcttgcg tagtttgagt acgagagctt
    45361 cggctaacag agcactaacc gatgccaaca ggatctgaac gagagtaccc caaccaaaaa
    45421 accacagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg
    45481 atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc atttagtcct
    45541 cgtttacaac cttttgctgg acggcttttt tggcctgaac gcgggcaata gccgcggcaa
    45601 ctgccgcttt gcgcggatca acctgttctt ctggttctgc attagcctgt tgctgttcca
    45661 gcttgcgtgc tttggcgcgt gcaatagccg ctgcgacggc agctttgcgc ggatcaacct
    45721 gttcttctgg ctccgcatta gcctgttgct gttccagttt gcgcgctttg gcacgggtaa
    45781 tagcggcttc gacggcggct ttgcgcggat cgaccggttc ttctggttcc gcattagcct
    45841 gttgctgttc cagcttgcgt gctttggcac gggcaatagc ggcttcgacg gcggctttgc
    45901 gcggatcgac cggttcttct ggttccgcat tagcctgttg ctgttccagc ttgcgcgctt
    45961 tggcgcgggc gatagcggct tcgacggcgg ctttgcgcgg atcgacctgt tgttctggtt
    46021 ccgcattagc ctgttgctgt tccagcttgc gtgctttggc acgggcaata gcggcttcga
    46081 cggcagcttt gcgcggatcg acctgttgtt ctggttccgc atttgcctgt tgctgttcca
    46141 gcttgcgcgc tttggcgcgg gcgatagctg cttcaacggc agttttacgt ggatcagtaa
    46201 cggttgctgc gtcgttagtt tgctgcagtt ctgcctgttt cgctctggct tgcgctttac
    46261 gggcttcccg tgctgcaata attgcactgt tatccgggcg ttcgcccgct ttaatcacaa
    46321 taggctgtgt agcctgggcc tgtttctctt tcacccgcgc cagagcggca gcaatcgcat
    46381 ctttatcttt ggctgcaggt tgaacggctg cgctcttatg tcgttcaagg cgagccgctt
    46441 tttcgcgctc cagacgagcc aggcgcgctt cgaaacgcgc tttggcttct gcggcgcgct
    46501 tttcttcctg acgaatagcc gcaatttcag ctttttcctg acggaaatat tgcaccaggg
    46561 gaatattgct cgggcaaacc cacgcgcaag ccccacattc aatgcagtcg gcaatgttat
    46621 gcgtggtagc tttatcgtgt tgctgacctt tgctgaacca gtacaactgt tgcggcaaaa
    46681 gatccgcagg gcaggcgtca gcacaggcgc tacaccggat gcagctttgt tcttcctgtg
    46741 gttcgccaag ctcattggca gagggagcca acagacagtt ggtaatcttt acgaccggga
    46801 catccagcca tggcaaggta aagcccatta gcgggccgcc cataatcacc atttgatcgg
    46861 cagaggggca gaatccggcg tcattcaata aatgacgcac tggcgtcccc agccgtgccc
    46921 agacgttgcc cgggcgagcg attgcttcgc cagtcagggt tacgacacgc tcggtaatcg
    46981 gctcgccatc aataacggca cgtttcactg cataagcagt gccgacgttt tgcattaata
    47041 cgccgatatc ggatgaacgc ccgccatgtg gaacctgctt cccggtcaga atgtaggtta
    47101 attgtttagc accgccagaa ggatatttgg ttggaatcac ccgcatagaa atatcgtgag
    47161 agtccgccag caccgcgcgc agcatggaaa tcgcctgcgg tttgttatct tcaatgccga
    47221 taagaatttc gcgtggctgc agaatatgcg caagaatgcg aataccctct acgacctgag
    47281 ccgcgcaatc ctgcatcaaa cggtcatcgg cggtaatgta tggctcgcac tcagcagcgt
    47341 tgataatcaa cgtctcaatc ttatctccgc cgccctgcaa tttaacgcct gtcgggaatc
    47401 ctgcaccacc cagcccggca acaccaaact gatgaatgcg ctcgattaac tcttcgcgac
    47461 ggcgagagcg ataatcggcc cagccgtctc gtgggatcca gcagtcttca ccatcggcat
    47521 caataatcac gcttaattca gctaaagctg aaggatgagc cgtagagtgg ggcgcaatag
    47581 ccgtaacggt acccgaggtg ggcgcgtgaa caggcagcat tttgccgcga ccacgggtaa
    47641 gcggctggcc gcgcaatact ttatcgccga cgctaacgca caactcacct tcagcgccaa
    47701 tatgctgttt cagtggaatg acaaaacgct gcgccagtgg tacctggcgc aggggtgtac
    47761 cgttggactg ggttttcatc tccggtggat ggatgccgcc gttgaaatcc cagattttat
    47821 tttttctgaa tgcagagaat aacttaagca tggtgttcca cgggaatgat acgcacggga
    47881 atggtgttca gatcccattt ccaggagtca ggtgtttctg cgaccggttg caacgagatg
    47941 cagtgcgtcg ggcacggatc aacacataaa ttgcagcccg tacagagatc actcattacc
    48001 gtatgcatgg cacgggtagc gccaacgatg gcgtctaccg gacacgcctg aatacattta
    48061 gtgcagccaa tacagttatt ttcatcaata accgccacca tccgcgcagg cgttagctct
    48121 tgcgcttcgc catccagcgg ctgcggctcg acattaagca actcggcaat ttttagcatc
    48181 acagcttcgc caccaggggc gcaacggttg attttttcac cgttacagct gatggcttcc
    48241 gcgtaggggc gacagccggg ataaccgcac tgaccacact ggctctgcgg taagatttcg
    48301 tcaattttct caacgaccgg atcgtcttcc accgcaaaac ggcgggaggc ataacccaga
    48361 atggcgccaa acgccaggcc cagcaggctc acggcggcaa cggcaatcca gatagcattc
    48421 attacaactt caccaaacca ctaaagccca taaaggccag agacataaga cctgcggtaa
    48481 ttaacgcaat ggcattaccg cgaaaaggtg ccgggacatc agccacagca aggcgttcgc
    48541 ggatggctgc gaagagaacc atcaccagcg aaaaaccgac agcggcggaa aaaccgtaca
    48601 gcgccgactg caagaaattg tgcccgagat tgatattcag caacgccacg ccgagcacgg
    48661 cacagttggt ggtgataagc ggcaaaaaaa tccccagcaa acggtaaagc accgggctgg
    48721 ttttgcgcac caccatctcg gtgaactgca cgaccacagc aatcaccaga ataaatgcca
    48781 gggtgcgcag gtaaatcaga ttaagtggga tcaaaatcca cgtatcgata agccaggcgc
    48841 aaatagacgc cagcgtcatc acaaacgttg ttgccagccc catgcccatc gcggtttcca
    48901 gctttttgga aacccccata aacggacaga gaccgagaaa cttgaccagt acaaagttat
    48961 tgaccagtac agttccgaca aacagtaaca ggtagtcagt catttttcgg cctgaaataa
    49021 aaaagccgcc tattatccgt taatccagag caggcgacaa caggttaact gtaaggttat
    49081 tacggattca caaacgtcgc tttcacccgc gacgagcgtt tgaaataggg gacgatcaat
    49141 gcggttgcca acaaagtaaa cagcaactgg cgaacggcta tgccatcttc cacgggtgaa
    49201 aaagcgaacg cttttaccgc cagcaatacg gaaataagca gccagataat atagtgttta
    49261 ggaacacaac gccgacgttt aaagaaagcg atggtcaacc acagtgtgta ataccacatg
    49321 gcgatagcgg taataaaaga aacgccccat aagatttgtg tcgtcagtgc ttgtgcgccg
    49381 agcgtttgaa atgtttgcgg agaagataac gcagcggtat acagcaacaa cgccagcgtc
    49441 gtacttaaca gagcgactaa aagccaggcc aacgggccaa gcaaccagcc tccaatacgt
    49501 tgtggcgttg tggtggtcat aggttctcca gaatagcgac gcaaacaggc aaatctaatt
    49561 ttaggggcgt tattataagg cttttataac cgatcgctac tttctttatt gaacatagtg
    49621 ccagacggac ttcggtacct ggccgaggtc aaacaaacgc ccgccagaaa ccagctctgc
    49681 gcgacggtga tcggcagcac gatacatatt gatcagttcc tgatcgtccg tcagggtgta
    49741 attaagatgg tcgtagagtt tttccagact ttcgagtgaa ctgattttgc gaaattttaa
    49801 taagtagtcc tgaacagtca taaaaggtcc ataaaataat taataccaaa ggggtaggta
    49861 ttattagtga tgataaaatt tcaccaaaag tgaacgcttg tgttattaca ataataagaa
    49921 tgcgctgatg gcaaatattc agggatgaaa aactgctatg aaaagagtaa cgcgctttac
    49981 tatccctgac aatcaccaac aacaccgaac aagataataa attcctggtt taatatccga
    50041 caagtgaaaa catgcaccct gacgggcagc atgtcgctct acaagcgcag ggcttacttg
    50101 tgttgtaccg tagtactctg ttcaggtggc tgatagttgt caatgtgact cgccacgcca
    50161 agaagaatga ctgaaacgac aagaacgatc caacctgtta attcaataag acgattcatt
    50221 acaactcaca tctctgttga ttgatccatt aacttcaggg ggtaaatgtt acttagcaat
    50281 aatagctcca gcaagatttt tactgaggtt tttgcgatat ttagcttttg tcgttggaaa
    50341 aatcgctatt tttttgactt aagttaaaca acatccctta ttgctggcag gttattaaac
    50401 tgttgagcga gggtaaagga tagtgtcaaa tagccatcat acttcaatga gaggcaatga
    50461 catgagcgac aacatccgtg ttgggttgat tgggtatggt tatgcgagca aaaccttcca
    50521 tgcgcccctg attgcgggca cacccgggct ggaactggcg gtaatctcca gcagtgatga
    50581 aacaaaagta aaagccgact ggccaacggt tacggttgtc tctgagccga agcatctgtt
    50641 taacgatccc aacatagacc tgattgtcat tcctacaccc aacgataccc atttcccgtt
    50701 agccaaagca gcgcttgagg cgggtaaaca tgtggtcgtt gataaaccct ttaccgtgac
    50761 actgtcacaa gcgcgagagc tggatgcgct ggcaaaaagc ctggggcgtg ttctgtctgt
    50821 attccataac cgtcgttggg atagcgattt cttgacgcta aaaggtttgc tcgcggaagg
    50881 cgtgctgggt gaagttgctt actttgagtc tcattttgac cgcttccgtc cgcaggtgcg
    50941 cgatcgttgg cgtgaacagg gcggcccagg cagcggtatc tggtacgatt tagcaccaca
    51001 tcttcttgat caggccatta cgctatttgg tttaccggtc agcatgacgg tagatttggc
    51061 acagttacgg cccggagcgc agtcgaccga ttatttccac gccatcttgt cctatccaca
    51121 gcggcgagtc attttacacg gtaccatgct ggcagccgct gagtcagcac ggtatatcgt
    51181 gcatggatcc cgaggcagtt atgtgaaata tggcctcgat ccacaggaag aacgtctgaa
    51241 aaatggcgag cgtctgccgc aggaagactg gggctacgat atgcgtgatg gcgtacttac
    51301 ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg acggtgcctg ggaattatcc
    51361 ggcttactat gcggcgattc gtgatgcgtt aaatggcgat ggtgaaaatc cggttccggc
    51421 aagccaggca atccaggtaa tggagttgat tgagctgggc atcgaatccg ccaaacatcg
    51481 cgcgactttg tgccttgcat gaaaaatgga tggtgcaatt tgcaccatcc atttttgtta
    51541 cttcgctgcg actttttctc gcagtgcgcg tttttcctct gcgttgagga aagccatttc
    51601 cagaccatta atctgtgcct ggcggatttg ctcgcgggat aacccagcag ctggcgcggc
    51661 aacggtatat tcgtgaatga tatccactcc ctgtacgccg ggatcgtcag tgttaatgct
    51721 ggcacgaatg ccatgctcaa ggaacgtttt cagcggatgt gcagccagct ctgccacggt
    51781 gctggtctga atattggagg tcagacagga ttcaatacca atttgttgct cggcgagaaa
    51841 atccatcagc gcccgatctt caatggcttt tacgccatgt ccaatgcgct ctgcccccag
    51901 ttcacgaatc gcctgccaga tgctttccgg cccggcagct tcgcctgcat ggacggtaat
    51961 atgccagccc gcatcacgcg cgcggttgaa gtgagaaaga aacagacttc ccgggaaacc
    52021 aagttcatca ccggctaaat caagtgcggt aatctggtca cggtgggcta aaaaggcctc
    52081 cagctcttgc tgacaggcgg cttcgccgaa ggttcggctc ataatgccga taagcttcgc
    52141 ctgcacacca aaggtgcggc aaccttcacg tacgccatcg atcaccgctt caacaacacc
    52201 tgcaacaggc agctgatgtg ccattgccat gtagcctggt gaaaaacgca gctcgacata
    52261 gtgcaggccg ttacgggctg catcttcaat gttttcaaat gccacgcggc gacaagcatc
    52321 aagagaggcg agaactttaa cgccccagtc aagtttggtc agaaagctca ccagatcggg
    52381 ttcgttggca atgacctgaa cgtggggaat cagtgtttcc agggattgtg caggaagcga
    52441 gatattatac tggcggccaa gttcaagaat ggtctgggga cgaatgttgc catcaaggtg
    52501 gcgatggata tcagttaatg gcagggtggt atcaatcatg gtcgcactct ttttctggat
    52561 ataaaagtgc gcaccattat aaaaacaaag cagggtaaaa atccacgatt gcgcaacgat
    52621 tttttacgtt gcgcaattgg ggttagcgaa cagcgcggat ggcgttaatt aatccagcca
    52681 cacctttttc cagtttcgaa cgtgggcagc cggcattgag tcggacaaaa ccacgacctt
    52741 cttcaccgta ggtatacccc ggcatgatcg cgactttttc ttgttcgata agtgcttttt
    52801 gcaacgcgtt gtcgtcaata ttcaacggac gtaaatcaag ccaggccaga taagtggatt
    52861 gcgggatctg ccagttgagt tcaggaaacg cggcgttcat tttatctgcg atatacgtca
    52921 ggttatcttt cagatagacg cgtaaggcat ccagccacgg tgcaccttgc tgataggcgg
    52981 cgatatgggc agttaacgcc agtaccgaag gggaagaaag cccatcacgt cctttcagtg
    53041 ccgataaata ggcatcacgg ctgctgctat tttctataat cccgtaagca ccggtcagcg
    53101 cgggaatatt gaaacttttc gatcccgacg tcagcaacgc ccagtctccc cgcgccacat
    53161 tactccaggg aatatgcggc tgctcgcccc aaaccatatc catatggatt tcatcggaaa
    53221 taacccgcac accatgacgc tcgcacaggt cagccatgat ctccagctca tcgcacgtcc
    53281 acacttttcc ggtaggattc tgtgggctac acaggagcat aattttacat tctggtttcg
    53341 ccaacacggc ttccagcttg cccatatcgc aaaaccagcc gtcagcctgc ttctctaaag
    53401 caacgggcat tactgtgcgc tgattacctt caatggcctt gtaaaatgcg tcataggcgg
    53461 gtgtgtggat caccacgcct tcacctgttt cagaccactg acgaatcagt tctgagacca
    53521 tatagataac agaagggcca tacaccaccg actgaggatc gatggcggtg taatgctggg
    53581 tggaaaacca gtgggcaata gccgcgagaa actcatcgtt cttccagcgg ctgtagccaa
    53641 acacgccgtg catcaggcgc tgattcagcg cctcgataat gcagggggca gtggcaaaat
    53701 ccatgtctga aatcgtgaaa ggtaacaggt cagcagtgcc gaaacggtca gcgacataat
    53761 cccactgcgt acaccatgtg ccatgacgat ccacgacctt tgaaaaatcg aacatatctt
    53821 atccttatgc ctggacagta tgcatcagac cggccatttc atcttttact gactgaactt
    53881 gtggcccgat aacaacctgc aggttatgtt gattaagttg tactacgcca attgcccgat
    53941 tgtccttcag tgcctgcaca ttaacaagcg acatatcttt cacagacaaa cgcagacggg
    54001 taatgcagtt atcgaggcta acaatattgt cggcaccgcc taaggcttcg aggattgccg
    54061 gaacgttgta acctgattta cccggcgcac cggcaacggc tttttcgatt gaactggcca
    54121 cttcgctatc gcgccccggg gttttcagat tgaagcgggt gatagcgaaa cggaagatga
    54181 cgtagtaaac gacaaaccag attgccacca ccactggcac catgtaccac ttggttgaca
    54241 gaccatgcaa aataccgaac accacgaagt cgatgatatt gccgtcggta ttaccgatgg
    54301 tgacgccgag cacagacatg acggtgaagc cgaggccggt taacagcgcg tggatgacat
    54361 acagaactgg cgctacgaac aggaacagga attccagcgg ttcggtcgtg ccgccaacga
    54421 cacaggcgat caggccagaa atcagcagcc ctttaatttt atggcgattt tccgggcgcg
    54481 cgcagtgata catcgctaac gctgcacctg gcagaccgcc gagaaacgca ggcattttgc
    54541 cttgcgaaag gaaacgcgtg gcgctttcag aaaaaccgtg agtggtcggg caactcaatt
    54601 gcgcctggaa gatggtcaat gcgccgctga cggtttgacc gcagacttcc tgcgtgccac
    54661 ctgcgtcggt aaagcgaatt aatgccacca gaatgtgatg cagaccaaac ggcaacagca
    54721 gacgttcacc ggtaccaaac agcatcggtc cgaaatcacc cgcgctattg atcatatggc
    54781 ctaagccgct aatacccatg gcgaaaatcg gccagactaa tggaatcacc aggccgacaa
    54841 ggcccatcac cagcgaggag atgattggta cgaagcgcgt accgccgaag aatgccagcg
    54901 catccggcag gcggatatta tggaatcgct catgcagcat ccagacgata ataccggcga
    54961 tcaccgcacc gaggatcccg gtgtcgatcg actggatccc aagaatgctc tggatgttat
    55021 tggctttcag aacggcggca tccgtggttg gcagaatgcc tttattggtc aaccagaagt
    55081 ttaccgcgag gttcattacc gcataaccga cgaagccagc gaatgccgct acgcctttgt
    55141 tttctcgtgc cagacccagc gggatggcga tacagaacat gactggcagg aaactaaaag
    55201 caaacgagcc aatcttactc atccaggtaa agatagcctg caacacgggg ttgcccagga
    55261 ccgggagcag ggtgatgaca tcgtggctac taagggaact accaatgccg agcataatgc
    55321 cgcagaacga caataatgcc acgggtaaca tgaaggtttt gcctaactgc tggaagaact
    55381 cccacagcgt gactttcggt gctgttttcg ccgtcataaa acgactcctc gtagagaaca
    55441 atctggattc agtaaattgc gcgagaagat aaaacgtttt atcaaatttt agtgaggcat
    55501 aaatcacatt acgcaacgat aatagcgggt ataagataaa taaaaggtaa aacgttttat
    55561 ctgtcacaaa atcagggagt aggtcatctg catggctacc gccaaaaaaa taaccattca
    55621 tgatgttgcg ctggctgcgg gcgtgtcggt aagtaccgtt tcgctggtgc ttagtggcaa
    55681 agggcgaatc tctaccgcca caggagaacg cgttaacgcc gccattgaag agctgggatt
    55741 tgtgcgcaat cgccaggcgt cggcgctgcg cggtgggcaa agcggcgtca ttggtttgat
    55801 cgtccgtgat ttatctgcgc cgttttacgc cgaattgacg gccggattga cggaagctct
    55861 ggaagcgcag ggacggatgg tttttttgct tcacggcggt aaagacggcg agcagctggc
    55921 acagcggttt tcactgttac tgaatcaggg tgtcgatggt gtggtaattg ccggggctgc
    55981 aggaagcagc gatgacctgc gacggatggc agaagaaaaa gctatcccgg tgattttcgc
    56041 ttcccgtgcc agttatcttg atgatgttga tacggttcgc ccggacaaca tgcaggctgc
    56101 acagttgttg acggagcatc tcattcgcaa tgggcatcag cggatcgcct ggctgggagg
    56161 gcaaagttcc tcattaaccc gtgcagaacg ggtggggggc tattgtgcaa ctctactaaa
    56221 atttggcctg ccgtttcaca gcgattgggt gttggagtgc acttccagcc agaagcaagc
    56281 cgcggaagct atcacggcgc ttttacgtca taacccgacc atcagtgccg tggtttgcta
    56341 taacgaaact attgcgatgg gggcatggtt tggtttgctg aaagcagggc ggcaaagcgg
    56401 ggaaagcgga gtcgatcgtt actttgagca acaggtttcg ctggcggcat ttaccgatgc
    56461 gacaccaacc acacttgatg atatacccgt tacctgggcc agcacgcctg cgcgggaact
    56521 tggtaccaca cttgcggatc gcatgatgca aaaaatcacc catgaagaga cgcattcacg
    56581 caatcttatt attcccgccc ggctcattgc ggcgaaataa tcctctcttt atctgctata
    56641 cctggtagtg tcctttcctc aagtttaatg ataaaaaatg tggcacacag gcggtgcctt
    56701 ttatttttac tgaagttcac aggaggttta tgtgtttaat tctgacaacc tgagactcga
    56761 cggaaaatgc gccatcatca caggtgcggg tgcaggtatt ggtaaagaaa tcgccattac
    56821 attcgcgaca gctggcgcat ctgtggtggt cagtgatatt aacgccgacg cagctaacca
    56881 tgttgtagac gaaattcaac aactgggtgg tcaggcattt gcctgccgtt gtgatattac
    56941 ttccgaacag gaactctctg cactggcaga ctttgctatc agtaagctgg gtaaagttga
    57001 tattctggtt aacaacgccg gtggcggtgg acctaaaccg tttgatatgc caatggcgga
    57061 ttttcgccgt gcttatgaac tgaatgtgtt ttcttttttc catctgtcac aacttgttgc
    57121 gccagaaatg gaaaaaaatg gcggtggcgt tattctgacc atcacttcta tggcggcaga
    57181 aaataaaaat ataaacatga cttcctatgc atcatctaaa gctgcggcca gtcatctggt
    57241 cagaaatatg gcatttgacc tgggtgaaaa aaatattcgg gtgaatggca ttgcgccggg
    57301 ggcaatatta accgatgccc tgaaatccgt tattacacca gaaattgaac aaaaaatgtt
    57361 acagcacacg ccgatcagac gtctgggcca accgcaagat attgctaacg cagcgctgtt
    57421 cctttgctcg cctgcagcca gctgggtaag cggacaaatt ctcaccgtct ccggtggtgg
    57481 ggtacaggag ctcaattaat acactaacga accggtaaac agccgtatgt gttgtttacc
    57541 gggatagcgt gtgcaattca tcaacgtctc tgctaaacaa ctggcagcca aatcacggcc
    57601 attggttaac caatagctgg atgaaaagca tacgaataga gtgtgccttc gcactattca
    57661 acagcaatga taggcgctca cctgacaacg cggtaaacta gttattcacg ctaactataa
    57721 tggtttaatg atggataaca tgcagactga agcacaaccg acacggaccc ggatcctcaa
    57781 tgctgccaga gagatttttt cagaaaatgg atttcacagt gcctcgatga aagccatctg
    57841 taaatcctgc gccattagtc ccgggacgct ctatcaccat ttcatctcca aagaagcctt
    57901 gattcaggcc attatcttac aggaccagga gagggcgctg gcccgtttcc gggaaccgat
    57961 tgaagggatt cattttgttg actatatggt cgagtctatt gtctctctca cccatgaagc
    58021 ctttgggcaa cgggcgctgg tggtcgaaat tatggcggaa gggatgcgta acccacaggt
    58081 cgccgccatg cttaaaaata agcatatgac gatcactgaa tttgttgctc agcggatgcg
    58141 tgatgcccag caaaaaggcg agataagccc agacatcaac acggcaatga cttcacgttt
    58201 actgctggat ctgacctacg gtgtactggc cgatatcgaa gcggaagacc tggcgcgtga
    58261 agcgtcgttt gctcagggat tacgcgcgat gattggcggt atcttaaccg catcctgatt
    58321 ctctctctct ttccgcgggc tggtgacaac tgagcccgcg tttcatactt taatttccct
    58381 gtacaaaata caatctcgcc ggagcgggga attcctccct aaaatattca ccgtagccat
    58441 atgtcatgag agtttatcgt tcccaacatg ctcgaacgaa cgttcggttg cttattttat
    58501 ggcttctgtc aacgccgttt taaagattaa tgcgatctat atcacgctgt gggtattgca
    58561 gtttttggtt ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt
    58621 tctcacagat aactgtgtgc aacacagaat tggttaacta atcagattaa aggttgacca
    58681 gtatttttat cttaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga
    58741 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
    58801 gcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
    58861 taacgatcag ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg
    58921 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
    58981 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
    59041 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
    59101 agtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
    59161 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactacg ccgggatcca
    59221 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
    59281 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
    59341 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggggcacc
    59401 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
    59461 gaactgtacg tcacagccaa aagccggaca gagtgtgata tctacccgct gcgcgtcggc
    59521 atccggtcag tggcagtgaa gggcgaacag ttcctgatca accacaaacc gttctacttt
    59581 actggctttg gccgtcatga agatgcggat ttgcgcggca aaggattcga taacgtgctg
    59641 atggtgcacg atcacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
    59701 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
    59761 gaaactgcag ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
    59821 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaggc gcacttacag
    59881 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
    59941 gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa
    60001 gcaacgcgta aactcgatcc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac
    60061 gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacggt
    60121 tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg
    60181 gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta
    60241 gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg
    60301 gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat
    60361 ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc
    60421 ttcacccgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc
    60481 atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc
    60541 gcaccatcgt cggctacagc cttggtgaca tcgccaataa cttcgccttc gcaatggggg
    60601 cgctcttcct gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca
    60661 ccatgctgtt actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg
    60721 tggacagtgt gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc
    60781 cgttaatgat cttcagcgtg ctggtattct gggtgccgac cgactggagc catggtagca
    60841 aagtggtgta tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata
    60901 ttccttatgg ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg
    60961 gcgcggctcg tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc
    61021 cgagcattaa gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg
    61081 tgctggcgat tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg
    61141 tggtacgtat cgtggcgcag ccgtcattga agatcagtct gcaaaccctg aaacggaatc
    61201 gcccgctgtt tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc ttcgcggtca
    61261 gcgcctcgtc gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc
    61321 tggtactggt gcaaaacctg gttggtactg tggcatcggc accgttggtg ccggggatgg
    61381 tcgcgaggat cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt
    61441 atctgctgtt cttctgggtt tccgtctggt cgctgccggt ggcgttggtt gcgttagcca
    61501 ttgcctcaat tggtcagggc gttaccatga ccgtgatgtg ggcgctggaa gctgataccg
    61561 tagaatacgg tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct
    61621 catttacccg taaatgcggt caggcaatcg gtggttcaat tcctgccttt attttgggat
    61681 taagcggata tatcgccaat caggtgcaaa cgccggaagt aattatgggc atccgcacat
    61741 caattgcctt agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc
    61801 cgctcacgga taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc
    61861 agcagcaatt aatcagcgat atcactaatt aatattcaat aaaaataatc agaacatcaa
    61921 aggtgcaact atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg
    61981 ccttacctct gcttacgcgg cgcaactggc tgacgatgaa gcgggactac gcatcagact
    62041 gaaaaacgaa ttgcgcaggg ctgataagcc cagtgctggc gcgggaagag atatttacgc
    62101 atgggtacag ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt
    62161 tgaaggcgga gcgtattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg
    62221 gtatcttgat ggtgataaaa gttttggctt tgccctcggg gcagtaaaaa taaaacccag
    62281 tgaaaatagc ctgcttaaat taggtcgctt cgggacggat tatagttatg gtagcttacc
    62341 ttatcgtatt ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg
    62401 agcattaggt tattgggctt taacgccaaa tattgatctg tggggaatgt ggcgttcacg
    62461 agtattttta tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca
    62521 gacgggaaaa tacgataaac atcgcgctcg ttctttttta gccgccagtt ggcatgatga
    62581 taccagtcgc tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag
    62641 tattctcgag aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg
    62701 cttttacgcg caactcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt
    62761 ggttagtgga caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta
    62821 tttgcgccat gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt
    62881 aagtcttgat cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt
    62941 aactcccaat ttacactgac atttgcaccg attgtgactc gcggctatga atccagtaaa
    63001 cgagatgtgc ggattgaagg cgcgggtatc ttaggtggta tgaactatcg ggtcagcgaa
    63061 gggccgttac aagggatgaa tttctttctt gctgccgata aagggcggga aaagcgcgat
    63121 ggcagtacgc tgggcgatcg cctgaattac tgggatgtga aaatgagtat tcagtatgac
    63181 tttatgctga agtaaaaaat aacgccggag agaaaaatct ccggcgtttt ggctgtgaat
    63241 tactgctgcg gaatagcggg tacgaccgga acatttaatg ccggcattgc aaacatacca
    63301 acgaaatctt ccagtggcat tttctgcccg tttaacgtta tctgaccgtt agtatattgc
    63361 aggctggtgg tgatggtatt gtcctgcaag gtggtcagac ggaacatctg ccccattgct
    63421 gatgcacctt caacttgctg tttcgccagt tttttcgctt gatcttcctg ataaccttcc
    63481 agcttcgcta cctgagtcat caactcagtt gccatatcca ccggaatggt cagtttcgca
    63541 tccagagatt taaccgaacg atctacttcc tgcgccagcg tttgcggcgc ttctttagtc
    63601 gttgccggat ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg actgtttttc
    63661 cagcttagcg gcgcgatagt aatcaccgga tcgcctttca acatcagcgg cagggcgcta
    63721 aagaaggctt ccgtcacttt ctcctgataa agttcgggat tgttggcaat ttctggctgt
    63781 gccagcagcg cctgagtttg cgcgttatat tgctggctaa actgatgcca ggcttcgcca
    63841 tcaatttggc cgacttttaa agtcagcttg ccgctgccca gatcctgatt ctgtaccttc
    63901 aggctgttta gcgagtaatc cagttggcta ttgatcgttt taccgtcatt gaccagatcc
    63961 gatttaccgc tgatctccat gccttccagc agtgccagtt ctttgccttc cactgaaatg
    64021 gtcatttttt ccagtgacag tttttggttt cctacgcgct caccaaaact tgccagcgtg
    64081 ctggaaccgt cggttttcag attattaaag gtcaactgta ctttctggtt gtattcgtta
    64141 accgcgtcta tccgaccact ttgcgcctcc ccggaaaggg agatagcttt gccgtctctg
    64201 tccgcattta actggaactc gccgccgcta aaggcgactt tttcatcctt ttgctcgtaa
    64261 tttagtggct tgagcgaaat atcggaactg gaatcaccgc tgtaaccaat gcgcgagtta
    64321 atctcaaaag gcgtttcacc ttttgccata tcaaacagtg gtttgcttac ttcgttatta
    64381 accagcgtgg tttgaattga tgccatcgac gggatcaggt tcagtttttt aagctgggca
    64441 agggggaagg gaccatgatc aaccgattcg ttgaagatga cgctctgacc gcttttaatc
    64501 cacggatttt cttttccggc aatgggtttc accaacagtt gcagctgact gctgaatacg
    64561 ccgcgatgat agttttgata actcacttcc aggttggatt caggagcggt cagtttgagt
    64621 tgcgcgttcg cctgcgcgac catgtcttcg agatgggttt caatcttctt gcctgtatac
    64681 catgcgccgc ctgtccagac tacgcctagc gcaacaatga cgcctaccgc taccagcgat
    64741 ttattcataa tgattatcca taaaatgaaa tcaggcggac tggccgcctg aaggtgttat
    64801 aagcctttaa taagcttagc aagagatgtt aattttttca gtaagctctt acagcttgtt
    64861 gtaaacccgt gctaaacggc cgtggccttt gacagtcacc ggtgattcgt tggcggcaat
    64921 aaacgctgat tcacccggtt taagctgtaa ctgctgagaa cctttccaca acgttgcatc
    64981 gccttcgacg cagaacaaaa tggcggcact ctgctggcta atggtggttt ctttatcact
    65041 aaggtcatgc agcgagaagg caaaatcatc aacaggaatc gggaagtcca gttctgcacc
    65101 ttgtttcacc ggctgggtca acaactggtt agccggtttg gcttcgaact tcacattggc
    65161 aaccagttcc ggaatatcaa tgtatttagg cgtcagaccc gcacgcagca cgttatcgga
    65221 gtttgccatc acttccagcg ccacgccttg caggtaagcg tgcggtgttt cagcgaacag
    65281 gaacatcgct tcgccaggat ttaatttcac cacattcagc aacagcggag agaacaggcc
    65341 gctgtcatcc gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt caccctgctg
    65401 actgtcgagc gccgatttta aaatcgccag cgcgcgggat ttttcttcac cctgcatatt
    65461 caacaggctg gcgaacagtt cgcttaaacg ttcggcatca ggctgttgta aaaagtgagc
    65521 aatcgccgga tgtgcacctg cgaccggctg gagtagggag acaatctcgg aaaattcacg
    65581 aaacgcgttc atcgcaagga aaggcgtcag cgcaaaaacc agctccggct tgtggttagg
    65641 atctttatag ttacgctcgg cggcatccat cgggatacct gcggcatttt ctttggcaaa
    65701 accgatttca gacttgtgtt tgtttggatg aacctgaatg gagagtggct gtgctgcgca
    65761 taatactttg aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct ctccgagcag
    65821 agtcgattta tcactctcaa tcacatcacg cagtgaaacg atatctccgg cggcattctg
    65881 cactcgtgaa ctgcttttcg gatgtgcgcc catccacagc tcggccatcg gctggctgga
    65941 cggattttcc ataccataaa gttcagtcaa cgccgttttg ctgccccagg catagttttg
    66001 cactgagtta atgagttttt gcatgatcaa tccctgtttt aatgtggaaa ttaatcccgc
    66061 tattaaagca agaatcctac gggaagtaac ctggagccgc aaaaagtcgt actagtctca
    66121 gtttttgtta aaaaagtgcg taggatattg ttactcgctt ttaacagggc aacggaacac
    66181 ccgcccagag cataaccaaa ccaggcagta agtgagaaaa caatgtcaaa caaacccttt
    66241 cattatcagg ctccttttcc actcaaaaaa gatgatactg agtattacct gctaaccagc
    66301 gaacacgtta gcttatctga atttgaaggg caggagattt tgaaagtcgc acccgaagcg
    66361 ttaactctgt tggcgcgcca ggcgtttcat gatgcgtcgt tcatgctgcg tccggcgcac
    66421 caacaacagg tggccgacat tctgcgtgac ccggaggcca gcgaaaatga taaatatgtg
    66481 gcgctgcaat tcctgcgtaa ctccgacatc gcggcgaaag gcgttctgcc aacctgtcag
    66541 gataccggca ccgcgattat tgttggtaaa aaagggcagc gtgtatggac cggtggtggt
    66601 gatgaagcgg cgctggcgcg cggtgtctat aacacttata tcgaagataa tctgcgctac
    66661 tcgcaaaacg cgccgctgga tatgtataaa gaagtgaata ccggcaccaa tctgccagcg
    66721 cagatcgatc tttatgccgt tgatggcgac gaatacaaat tcctctgtat cgccaaaggt
    66781 ggtggttcgg caaacaagac gtatctctat caggaaacca aagcgttact gacgccgggg
    66841 aaactgaaaa attacctggt tgagaagatg cgcacgctgg gtacggcggc ctgtcctccg
    66901 tatcatattg cgttcgttat tggtggaact tctgcagaaa cgaaccttaa aacggtgaaa
    66961 ctggcttccg cgaaatacta tgatgaactg ccaacggaag ggaatgagca cggtcaggcg
    67021 ttccgcgatg tggaattgga aaaagaattg ctgatcgaag cgcaaaatct tggtctgggt
    67081 gcgcagtttg gcggtaaata cttcgctcac gacatccgcg tgattcgcct gccacgtcac
    67141 ggcgcatcct gcccggtcgg tatgggcgtc tcctgttctg ctgaccgtaa tatcaaagcg
    67201 aagatcaacc gtcaggggat ctggatcgaa aaactggaac ataatccagg caaatatatc
    67261 ccggaagagc tgcgcaaagc gggagaaggc gaagcggtgc gcgttgacct taaccgtccg
    67321 atgaaagaga tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt atcgcttaac
    67381 ggcacgatta tcgtcggtcg tgatattgct cacgccaaac tgaaagagcg gatggataac
    67441 ggtgaagggc tgccgcagta catcaaagat catccgattt actacgcggg tccggccaaa
    67501 acgccggaag gttatgcctc cggttctctt ggcccaacga ccgccggacg gatggattct
    67561 tatgtcgatc aactgcaagc gctgggcgga agtatgatca tgctggcgaa aggcaaccgc
    67621 agccagcagg tgacggatgc ctgtaaaaaa cacggtggct tctaccttgg cagtatcggt
    67681 ggtccggccg ctgtattggc gcagggaagt attaagagcc tggaatgtgt tgaatatccg
    67741 gaactgggaa tggaagccat ctggaaaatt gaagtggaag atttcccggc gtttatcctt
    67801 gtggatgata aaggaaatga cttcttccag cagatacaac tcacacaatg cacccgctgt
    67861 gtgaaataaa cagagccgcc cttcggggcg gtttttttac atggcacgaa agaccaaaca
    67921 tttgttatca aatagtaaat aatgagtgag ctaaaagtta cttaacgaaa gcaaaacaga
    67981 aagaaaaaat taatcaggtg aggagtaggc catgaataca gtacgcagcg aaaaagattc
    68041 gatgggggcg attgatgtcc cggcagataa gctgtggggc gcacaaactc aacgctcgct
    68101 ggagcatttc cgcatttcga cggagaaaat gcccacctca ctgattcatg cgctggcgct
    68161 aaccaagcgc gcagcggcaa aagttaatga agatttaggc ttgttgtctg aagagaaagc
    68221 gagcgccatt cggcaggcgg cggatgaagt actggcagga cagcatgacg acgaattccc
    68281 gctggctatc tggcagaccg gctccggcac gcaaagtaac atgaacatga atgaagtgct
    68341 ggctaaccgg gccagtgaat tactcggcgg cgtgcgcggg atggaacgta aagttcaccc
    68401 taacgacgac gtgaacaaaa gccaaagttc caacgatgtc tttccgacgg cgatgcacgt
    68461 tgcggcgctg ctggcgctgc gcaagcaact cattccgcag cttaaaaccc tgacacagac
    68521 gctgagtgaa aaatcgcgtg catttgccga tatcgtaaaa atcggtcgaa cccacttgca
    68581 ggacgccacc ccgctaacac tggggcagga gatttccggc tgggtagcga tgctcgagca
    68641 taatctcaaa catatcgaat acagcctgcc tcacgtagcg gaactggctc ttggcggtac
    68701 agcggtgggt actggactaa atacccatcc ggagtatgcg cgtcgcgtag cagatgaact
    68761 ggcagtcatt acctgtgcac cgtttgttac cgcgccgaac aaatttgaag cgctggcgac
    68821 ctgtgatgcc ctggttcagg cgcacggcgc gttgaaaggg ttggctgcgt cactgatgaa
    68881 aatcgccaat gatgtccgct ggctggcctc tggcccgcgc tgcggaattg gtgaaatctc
    68941 aatcccggaa aatgagccgg gcagctcaat catgccgggg aaagtgaacc caacacagtg
    69001 tgaggcatta accatgctct gctgtcaggt gatggggaac gacgtggcga tcaacatggg
    69061 gggcgcttcc ggtaactttg aactgaacgt cttccgtcca atggtgatcc acaatttcct
    69121 gcaatcggtg cgcttgctgg cagatggcat ggaaagtttt aacaaacact gcgcagtggg
    69181 tattgaaccg aatcgtgagc gaatcaatca attactcaat gaatcgctga tgctggtgac
    69241 tgcgcttaac acccacattg gttatgacaa agccgcggag atcgccaaaa aagcgcataa
    69301 agaagggctg accttaaaag ctgcggccct tgcgctgggg tatcttagcg aagccgagtt
    69361 tgacagctgg gtacggccag aacagatggt cggcagtatg aaagccgggc gttaatctgc
    69421 aacatacagg tgcagccgtg gaatgatcaa acgaagcggc tgcgcctgag gtttataacg
    69481 gtgctgcaca ttgtcggcat cgtaatttaa caactcacca acgtccggca cgcccgcacc
    69541 attatcccga ttaatcagtg caatcagcgg tgtagggcag gcgtgttgga cttgtttttg
    69601 atctcctttg taccagacgc gggcaatcgg ctgcaccttc accggacgtt tgatttttaa
    69661 cttcgcattc tgtggcaggg cagcgatatc ctgatactct cgctccagtt ttctttgcca
    69721 ctcctcgcgc gtccacggtg cgacactgcg tggtgatttc aggctttttt ccagctgtgc
    69781 caggacttca tcacgatgca aattcttaat gatatgttta ttagcccaac caaagcgtaa
    69841 agtggcggga tcgtgcagaa cggtgagcgt gcggtaagca ttaagggtga tcagccccgg
    69901 caaatgacga tgcacccatt caaaacgtgc cgcggtgggg agttctgatt caaccgtgac
    69961 gatatgctcg aacgtggtct tgagtttatt gatgtgctga atatgactga ccaacgctgc
    70021 ttgcgaaagg ttatcgacct ggtaacacaa cacgccaggc agacggacag ctgctttgct
    70081 gctgcgattt tcggactgtt gttgaataaa taaatgacgg aaatgacgca acgcctgcga
    70141 ctgcgcgtcg ttgccgagat gttgtttcac ctcaatacga ttaagcggat tatgctcatc
    70201 ctcttttttt acctccggca aagagaacac gcgggcaacc aatagcttgt gttgctcaag
    70261 atgagcggca aatgcagcca gctcttgttc catctggcga aaagtagtgt tgagtcggtc
    70321 tacgagatcg taacgcgcca taatattaac cactttagtt acaacatcct taatttatag
    70381 cacagtggtg gtgacttgac caccgtgcgc gtagtgtcaa gcagaggtaa attgcgggat
    70441 gttatgccat aacggccagc taaacgagaa gcgggcacca cccagttcgc tggtgtcaca
    70501 attaaccgta ccgcccattg ccagtgctat agagtggaca attgccagcc ccagcccgca
    70561 gccgccggtt gagcgatccc ggctgggatc gaggcgaaca aaaggttcaa agatatgttc
    70621 gcggttttct ggggcaatcc ctgggccatc atcctcaaca attaatgtcg ctctattccc
    70681 cgacagtagc aggctggttt caaccgttga atggcagtag cgcagggcat tattgagcaa
    70741 attatccagc acgcgctcca ttaagcgcat atccaacgcc gcataatggc cttgcatgag
    70801 cgtttttatc cgtaccgttt tatcgggcgt tactgcctga atatctgcca gatgcgttga
    70861 cagccacaac ggcaggtctg gttcgctaag atgaagctcg ttttgtgggc gatcgagtcg
    70921 ggcataagtc agcagctctt caattaaagc ttcaagttga ctgatatcac gattcaacgc
    70981 ctgggattcg gcggcgctca ggttatcgct catctccagt cgataacgca ggcgcactaa
    71041 cggtgttcgc agttcgtgag cgataccgtc aataagctgt tttttgctgg caatcaaggc
    71101 gttgatattg tccgccatct ggttaaatgc gataccaagt cgttcaaagc tcgaaccctc
    71161 atcaaagtgg atacgttcat tgagatgccc atcgccaaat cgttgcgccg ctgcttccag
    71221 ttttaacata tcctgccagt gcggacgcat ccagataaac accggaaagg cgagggaaat
    71281 agcaataaaa gcgatcaggg cgatatccag caatcgcatc tgatggagat aataaagata
    71341 aggaacagga ccaactgcca gcacgtagtg gctgcgcggg atacgctgca aaaaagtgta
    71401 ctgatcgtcc agggcgacaa tttcgccgcc acgcagccgg tgcatggaaa tatcatcaag
    71461 atggtattta ctcagtggct cgactcgcag atcgaaagag agatttaaat ccatctcttt
    71521 cagcgtctta ccccagtcat gtggggggat ctcacgcaat tcgctgcgca tcagatacag
    71581 cgaactgttc atcaaatcat ccagcgactg tttgcccgcg cgttcggcgg taaatttgta
    71641 caccaaccca accagcagag acatcacaag gaagcagaca aacaataaca ggtaaaactg
    71701 gataaacagt tttttcatcg cttattccca tgcatgaggc gcaaaaagat agcctttgtt
    71761 acgcacagtt ttaatgcgat aaggttcagc ggcgttatcg agcagttttt ttcttaaccg
    71821 cgaaatagcc acgtccacgc tacgatccag gccgtcataa ctgacgccgc gtaaattttt
    71881 cagcaatgca tcgcggtcca tgatttgccc ggcatgggta gctaattccc acaataattc
    71941 gaaatcagct gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatagg
    72001 atcgatggtc aacgtgccga aatgcagggc tttgtaggga gtcagtgacg tttcctgaag
    72061 acctttggtc agcgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctaacaa
    72121 aacagcaggg ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat
    72181 gtggttcata tcgctatcga gagaggttag aagaacaatc ggtccggacc actttgcgcg
    72241 taaatcacga caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa
    72301 atccggattt tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggct ctacggtaac
    72361 ctgtatatca tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc
    72421 cacaaatacg atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc
    72481 gctgttttta cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg
    72541 acttgttatg gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg
    72601 cgctggtagt gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc
    72661 tgattccact tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg
    72721 gcattgaagc cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg
    72781 tttacctggt gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg
    72841 gttctgttgc gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc
    72901 attaacgcag atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg
    72961 taagtaagta atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcaatg
    73021 atttattcag cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg
    73081 atggcgcaat agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca
    73141 tattatccag tgccgctttg cttgcagcat acgcaatatg tttgtcgcta ccgcgctcca
    73201 ccacataatc ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc
    73261 tttccagcgc atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca
    73321 gtacgtcggc cagtggcgca cccggttttt ccgccatcca cgcactggcg ttatgcaaaa
    73381 tagcacgcag accatgggtg ctttttagta cttcatcggc aaacgccatc acaccgtcgt
    73441 tggtcgaaaa atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg
    73501 gatagtgtgt ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga
    73561 gggcgaggcc gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac
    73621 ccatcgttat ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg
    73681 tcgccggaac tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta
    73741 cattatcatg tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca
    73801 gaacaacgga taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa
    73861 tgcaggcacc gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg
    73921 cgccgaccag gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt
    73981 aatcggaacc ggtcagccag atgagtacca gacaaatttg cacgcagatg tttgtcaacc
    74041 acagtgaggc ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt
    74101 tatgagtggc agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc
    74161 cgcaaacgga aacgatcaaa ccggcagcga tgatgatttc gccccacggt cccatcattt
    74221 ccaccatcag accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca
    74281 ctcccagcga aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca
    74341 gtgtcgcttt gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt
    74401 ccacaccaat gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag
    74461 gtacgccaag tgcaagtccg gtgaagtcga gcttgaagat atccagtttg aacatcatca
    74521 tcgccagcac aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa
    74581 tgctggcagc ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg
    74641 atgcaccgac tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt
    74701 ccgtaaaaaa gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgactg
    74761 cgcacagcca gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg
    74821 cataggtaaa gataccgccg tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa
    74881 aggccagcaa taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc
    74941 tggcaactgc cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta
    75001 ataccagcgc ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa
    75061 aacgtgataa gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa
    75121 gcgcgggatt ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta
    75181 aaaatgcgaa aggcggcatc aagccgcctt ataagagtaa ctgaatagtt atttatacag
    75241 atctgcactg acggtcaggt tattaccacg ttcctgccac tggcgggtga tgtggtaata
    75301 tttagcacct ttcttcgcgg ctcgtttcgc aacctgatag gagacttcgg tcatgttgcc
    75361 gtagttgcca gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt
    75421 cagttcttcg actttagtgc catccggaag cgtgacagtg taacgcccgc cttttgatga
    75481 ctgggtttca aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg
    75541 gatctcaact ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga
    75601 atctgccggg atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat
    75661 gaatgcagta atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa
    75721 agagtaggca cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct
    75781 acgatagaag ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt
    75841 cggcagttcg acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc
    75901 ggctttatag aggtcagcga ccacacgcca gttaccgctg ttcccaaaat cagaagtgtc
    75961 gacaacataa aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc
    76021 ttcgccaata gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc
    76081 cgcttgctcc ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag
    76141 tgccgacgcc aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac
    76201 caggtactgg tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa
    76261 ctgtctgcgt catttttcat atcacattcc ttaagccaat tttaatcctg ctcaaatgac
    76321 cgtctatgct taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat
    76381 aagttaattt aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg
    76441 tcgaagacaa ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct
    76501 gaatcgttaa tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg
    76561 cataattttc agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt
    76621 agctcgtaca tgagcggctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc
    76681 gatggaaggg aatatcatgc gaattggcat accaagagaa cggttaacca atgaaccccg
    76741 tgttgcagca acgccaaaaa cagtggaaca gctgctgaaa ctggggttta ccgtcgcggt
    76801 agagagcggc gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc
    76861 tgaaattgta gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc
    76921 gttagatgat gaaattgcgt tactgaatcc tgggacaacg ctggtgagtt ttatctggcc
    76981 tgcgcagaat ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat
    77041 ggactctgtg ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa
    77101 tatcgccggt tatcgcgcca ttgttgaagc ggcacatgaa tttgggtgct tctttaccgg
    77161 gcaaattact gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgcgggtgt
    77221 tgcaggtctg gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga
    77281 cacccgcccg gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga
    77341 ttttaaagag gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat
    77401 caaagcggaa atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac
    77461 cgcgcttatt ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat
    77521 gaaggcgggc agtgtgattg tcgacctggc agcccaaaac ggcggcaact gtgaatacac
    77581 cgtgccgggt gaaatcttca ctacggaaaa tggtgtcaaa gtgattggct ataccgatct
    77641 tccgggccgt ctgccgacgc aatcctcaca gctttacggc actaacctcg ttaatctgct
    77701 gaaactgttg tgcaaagaga aaaacggtaa catcactgtt gattttgatg atgtggtgat
    77761 tcgcggcgtg actgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt
    77821 atcagctcag ccgcaggtgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg
    77881 tgcctgttca ccgtggcgta aatacgcgtt gatggcgctg gcaatcatct ttttcggctg
    77941 gctggcaagc gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg
    78001 cgttgtcggt tattacgtgg tgtggaacgt atcgcacgcg ctgcatacac cgttgatgtc
    78061 ggtcaccaac gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg
    78121 cggctgggtt agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg
    78181 tggcttcacc gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata
    78241 tgtctggagg attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc
    78301 tggccgggct ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga
    78361 tggcgattgc gctaatcgcc accatttttg gaccggatac gggtaacgtt ggctggatct
    78421 tgctggcgat ggtcattggt ggggcaattg gtatccgtct ggcgaagaaa gttgaaatga
    78481 ccgaaatgcc agaactggtg gcgatcctgc atagcttcgt gggtctggcg gcagtgctgg
    78541 ttggctttaa cagctatctg catcatgacg cgggaatggc accgattctg gtcaatattc
    78601 acctgacgga agtgtttctc ggtatcttca tcggggcggt aacgttcacg ggttcggtgg
    78661 tggcgttcgg caaactgtgt ggcaaaattt cgtctaaacc gttgatgctg ccaaaccgtc
    78721 acaaaatgaa cctggcagct ctggtcgttt ccttcctgct gctgattgta tttgttcgca
    78781 cggatagcgt cggcctgcaa gtgctggcat tgctaataat gaccgccatt gcgctggtat
    78841 ttggctggca tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc
    78901 tgaactcgta ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc
    78961 tgattgtgac cggtgcgctg gtcggttcct cgggggctat cctttcttac attatgtgta
    79021 aggcgatgaa ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt
    79081 ctactggcga tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg
    79141 aactgttgaa aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc
    79201 aggcgcaata tcctgtcgct gaaattaccg agaaactgcg cgctcgtggt atcaacgtgc
    79261 gtttcggtat ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg
    79321 aagcaaaagt accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg
    79381 ataccgatac cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg
    79441 atccgaagag tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga
    79501 ttgtctttaa acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca
    79561 aggaaaacac ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag
    79621 ctctgtaacc ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga
    79681 acggcgatgc tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgttaac
    79741 ggcacggaaa gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa
    79801 aataccacca atgtggaaag tcccagccca cgccccatga tgcgtggctc cagaatattg
    79861 ccgaagacca gattaatcag cagatatccc gccagcacca acaacgcttc gtagaagcca
    79921 ttaaacacca gtacctgagc aatagggggg attgccgcga gaactgaacc aatattaggg
    79981 atgtaattaa gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagggcg
    80041 gcgagcatcg cccatgcgac caggccggtg atgatgctga tggctgtttt cagcaccaga
    80101 taatgagaca cactgtcaat cgcacgctga atcgccgcca tcccttcaac cggacgcgcc
    80161 atcatttgct taaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc
    80221 agcagcaata aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc
    80281 aacgtcatcg ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc
    80341 cctacgcgtt gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat
    80401 tgcggtaacg tccgcgtcaa ctcgttgagc gtggaaccca gataagctaa cagcaacacc
    80461 atagccatca cgatgatggt catcaaaatc gacactgcca gtacacgcgg cacacgccag
    80521 cggaccatgt gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac
    80581 ggcacgatga tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac
    80641 ataatgacga tttttaggcc attgagcgtg atgagcggct ttgccatgct tgtccccgtt
    80701 ttttcttcct ttgctaataa taatgtcttt ttcgctccgt catcataacg aaaatcaaac
    80761 cgctcaggta aagaagtgaa aatattttga gttaattctt aagctatgat acaaatcagg
    80821 cgtgttcaac taccgaggac aattatcatc cgcgatgacg agaagcaaca ctgcggataa
    80881 ttgtaatatt atggacaatt tgttcaggac gttattttct acatttaccc atcttcgtac
    80941 ctcatccact atcttgctcg taggtgagca gcactggcgc aacgcgcttt agtcctttct
    81001 tctgcctaag cttgccctat acgcggggct tgaccacttt actttggttt cgctgaatta
    81061 agcgaaaatt aaaataattc tcttgcagga gaaggacaat gtatatttat tggattttat
    81121 taggtctggc tattgctaca gaaattaccg gtacgctgtc tatgaaatgg gcgagcgtca
    81181 gtgagggaaa tggcggcttt attttaatgc tggtgatgat ttctctgtcg tatatatttc
    81241 tctctttcgc tgttaaaaaa atagccttag gtgtggctta tgcgctgtgg gaaggtatcg
    81301 gtattttatt tattaccttg tttagcgttt tgttattcga cgaaagttta tcgctgatga
    81361 aaattgccgg gttaaccacc ctggtcgccg ggatagtgtt gatcaaatca ggtacccgta
    81421 aagcacgtaa acctgaactg gaggtgaacc atggcgcagt ttgaatgggt tcacgccgcc
    81481 tggctggcat tggcaatcgt gctggaaatc gttgctaacg tctttttgaa attttctgac
    81541 ggctttcgtc gcaaaatatt tggcttgctc tccctggcgg cggtgctggc tgcctttagt
    81601 gcgctttctc aggccgttaa agggatcgac ttgtctgtcg cttatgcatt gtggggcggg
    81661 tttggtattg ccgccacgtt agccgcaggt tggatcttgt ttggtcaacg gttaaatcgt
    81721 aaaggctgga ttggcctggt cttgctgttg gctggaatga tcatggtgaa acttgcctga
    81781 tgaagacgct gcccgcgctg tcgggcagcg tttgaacatt atttttgcga cagttgatcc
    81841 agcttgtcgc gaaaaccggt aacagaaatg gcccggttat cggcgcgcca gcgatctttc
    81901 gcggcaggag ccgaactttg caccccaatt aattgccagc cgtcatcggt atgcaacatc
    81961 agaggcgaac cgctgtcacc cggcaaggta tcgcactgat gtgacatcac cgacgtttgc
    82021 gcccagccag tcacttcaca gttttgatga ctgtacaacg tatcgagatg atcttcaggg
    82081 tatcctgcct gagtcacttt acgacctgcc gcttttaatg cggcggtaag cgcggcttta
    82141 tctccctcaa ataacggcaa cggcgtaatg ccagaagggg gattacgtag cacaatcaat
    82201 ccgaagtccc acggcgcggc tgcgggaggt acaatccaac catccccatc tgcttttaac
    82261 cgctttccca gtgtcggatc aacgcggcct tctatgtcgt ggatgtcata gcgccaaaga
    82321 cctttatttg acacaaaacg cagcgccact gctttatcgg ctttaccctt tggtggcgtc
    82381 aataaacagt gtcctgccgt taatgccaga ttgggtgcaa tcagcgtcgc cgtacataaa
    82441 ttgccgctgg ccgtttccag ttgcccaacc gcatcccacg gtgattgggt cgtgtcattc
    82501 actggcacac gatcatcatg accaaaaaac agggtgctga cctcatcgtt tgccgatttg
    82561 gcaacgtctg gtttatctgc aaacacaaaa gcagacgtca aactaattgc acccaacact
    82621 acagcaatgg ttgtacgcat atcacactct ggtgggtaat tatgattatt aaaagcaaac
    82681 cctcataaat actatagacg ggacgggatg aaagtgggag taaaatcaga taactacgat
    82741 caggaaagat aaaaacgggc ggcaatcagt gcgcataaaa taaggatgat aagaatgaac
    82801 tcaaagcgat aacggcccac catactaccc tccgctaaag gcggtgctaa acgttacccg
    82861 cttagcaccg taattttcag gcgcgagggg gcgcgccagc attactgttg aaaacttacg
    82921 ctgcgggttg tgcagcaggt tttgccggtt gctgatggct gtgtttcttg gcgtgtttct
    82981 tcgctgcctg cgctttttgt tctggggctt tctgttcagc tttcgtattt ttatgatgct
    83041 ttttagccgc ctgcgctttc tgggcaggtg ctgctttatg ctgtttttta tgatgtgtag
    83101 ttttcgccgg cgctgctttg gtggtcgtcg cagtcggagc aggtgtggtc gcagtctctg
    83161 cagcaaaggc ggcagaagac agacccatag cagcggcaac aaccagagct aatacttttt
    83221 tcattgtcat accctcaatt tgttttttca tttaacccca ctgcggggcc gttgaaagaa
    83281 ctatatccct gggaatggca gacttccgtg agtggttggt ttcagcgtgt aacgatatgt
    83341 acaaacgctg aataaattac agcgttgata ataacgtatt gtgacttaag gaaaatttag
    83401 ctaccaataa tagtagtctt gatcgggtta actattttac tacttacaaa gcggtagaat
    83461 aactgcgcat cagtaataaa tgacacacag caaaatgaat ccgtttattt gggtacttat
    83521 aatcctgatg acgctagacg cgctgcggga attggctggc gcttcgtcta ttttaggatg
    83581 gctattaacg ttggtttgag caggcaataa gtccggacgg gtatttaccg cagtccggac
    83641 ttatttttca ggcgtgcaga cgacgatgca aacgcgtccc gaccagcaga gcaatgacca
    83701 gcatcagagc aataaatgcg ccgacgccgt tccagccata gttatgccag aaaacaccac
    83761 ccagcgtccc ggcaatactc gaccccagat agtaactgaa cagatacagc gaggaggcct
    83821 ggcctttagc gcgttttgcg cgggggccga tccagctgct ggcaactgag tgggctgcga
    83881 agaatcctgc tgagaagagt aacattccgg caaagatcag ccacagcgag ctgaataagg
    83941 tcatcagtaa accaaacagc ataacccccg tcgaaaacaa catcactgga ccacgcccat
    84001 agcgggtggt catggttccg gctttgggtg agctccatgt accggtcaaa taagccagcg
    84061 ataataagcc aaccacggcc tgactgacat gccaggggga gagcatcaac cgatagccga
    84121 tgtaattaaa cagcgtgacg aacgacccca tcagcaaaaa gccttctgcg aacaataacg
    84181 gtaatccccg gtcacgccag tgcagacgaa agttgataaa caacgactta ggtcgcagcg
    84241 aagtcgggcg aaaatggcgt gattccggga ggattttcca gaacatcaac gccgaggcta
    84301 gcgcgaaaca accgatagcc gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac
    84361 cactaattaa gcgtccgctc atgccgccaa ttgagttgcc gctgatatac aaccccatcg
    84421 aaaaggccac gaaactgggg tggatttcct cgctaagata agtcatgcca acagctgcca
    84481 cgccacttaa cgaaagccca atcaaggcgc gcataatcaa aatgccgtgc cagctggtca
    84541 tcattgtcga aagtaacgta caaatggagg ccaatagtag cgccgttacc atcactggtt
    84601 tgcgaccaat ggcatcggat agggggccag taaacagcaa accaatagcc aacatcgccg
    84661 tggaaatgga cagtgaaata ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca
    84721 ccggaaggat aggttgcaca caatagagaa gtgcaaatgt tgccagtccg gcagagaaca
    84781 gcgccagggt gacgcgcata aattgcggcg taccgcgttt aataaattga tttggctgag
    84841 aaatgctttg cttgtcagtg tcgcttgccg gagcgccatc aacagttgta gtacggctca
    84901 cttgaaatcc ttgctaaata tgcctgtgga tcaggcttat acataggtta ggaaaatcga
    84961 attgttctgt ctaatatatt aataatctca aataagatgt tttaaatatg aatattgaac
    85021 ttcgtcatct gcgttacttt gttgctgttg cggaagagct gcatttcggg cgcgccgctg
    85081 cccgcctgaa tatttcgcaa ccgccgctaa gtcagcagat tcaggcgctg gagcaacaaa
    85141 ttggtgcccg actgctggca cgaaccaatc gcagtgtatt gctgacggca gcaggaaaac
    85201 actttcttgc agatagtcgg caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg
    85261 aaagactgca tcagggtgaa gcgggggagt tgcgcattgg ttttacttcg tcggctcctt
    85321 ttattcgggc ggtgtccgat acgttatcgc tgtttcgccg agattatccc gatgtccatt
    85381 tacaaacccg cgaaatgaac actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg
    85441 atatgggatt gctgcgtaac acagcgttac cggagacgct tgaacacgca gtcatcgtcc
    85501 atgaaccgct tatggcgatg atcccgcacg atcatcccct ggcaaataac ccgaatgtaa
    85561 cgctggctga actggcgaaa gaaccctttg tcttttttga tccgcacgtc gggacagggc
    85621 tgtatgacga tattctcggg ctgatgcgac gttaccattt gacgcccgtc atcactcagg
    85681 aggtgggcga ggcaatgacc atcatcggtc tggtttccgc cggtctgggt gtttcaattt
    85741 tgcctgcgtc atttaaacgt gttcagctca acgaaatgcg ctgggtgccg attgctgaag
    85801 aggatgcggt ttctgaaatg tggttggtct ggccgaaaca tcatgaacaa agtccagctg
    85861 cgcgtaactt tcgtattcat ctgctgaatg ctctcaggtg agggaaattt cagcgaaaaa
    85921 gcccgaaaaa tgtgctgtta atcacatgcc taagtaaaaa tttgacgaca cgtattgaag
    85981 tgcttcacca tagcctacag attatttcgg agcgcgaaaa tatagggagt atgcggtggt
    86041 tgctgaaaac cagcctgggc acattgatca aataaagcag accaacgcgg gcgcggttta
    86101 tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat ctttcccgtc tggcgcagct
    86161 ggctcctgcc agtatcacta aaattgtccg tgagatgctc gaagcacacc tggtgcaaga
    86221 gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg gtggggctgg tggttgaaac
    86281 tgaagcctgg cactatcttt ctctgcgcat tagtcgcggg gagattttcc ttgctctgcg
    86341 cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa ctggcgttaa aagatgactc
    86401 gccattgctg gatcgtatca tttcccatat cgatcagttt tttatccgcc accagaaaaa
    86461 acttgagcgt ctaacttcga ttgccataac cctgccggga attattgata cggaaaatgg
    86521 cattgtacat cgcatgccgt tctacgagga tgtaaaagag atgccgctcg gcgaggctct
    86581 ggagcagcat accggcgttc cggtttatat tcagcatgat atcagcgcat ggacgatggc
    86641 agaggccttg tttggtgcct cacgcggggc gcgcgatgtg attcaggtgg ttatcgatca
    86701 caacgtgggg gcgggcgtca ttaccgatgg tcatctgcta cacgcaggca gcagtagcct
    86761 cgtggaaata ggtcacacac aggtagaccc atatggtaaa cgctgttatt gcgggaacca
    86821 cggctgcctc gaaaccatcg ccagtgtgga cagtattctt gagctggcac agctgcgtct
    86881 caatcaatcc atgagctcga tgttacatgg gcaaccgtta accgtggact cattgtgtca
    86941 ggcggcattg cgcggcgatc tgctggcaaa agacatcatt accggggtgg gcgcgcatgt
    87001 aggacgcatt cttgccatca tggtgaattt atttaaccca caaaaaatac tgattggctc
    87061 accgttaagt aaagcggcag atatcctctt cccggtcatc tcagatagca tccgtcagca
    87121 ggcccttcct gcgtatagtc agcacattag cgttgagagt actcagtttt ctaaccaggg
    87181 cacgatggca ggggctgcgc tggtaaaaga cgcgatgtat aacggttctt tgttgattcg
    87241 tctgttgcag ggttaacatt ttttaactgt tctaccaaaa tttgcgctat ctcaatttgg
    87301 gccaggaaag cataacttag actttcaagg ttaattattt tcctggttta tatttgtgaa
    87361 gcataacggt ggagttagtg atgctgaagc gtttctttat taccggtaca gacacttctg
    87421 tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt agcctcccag ggaaaaacgg
    87481 ttgcgggata taaacccgta gcgaagggga gcaaagagac acccgaaggg ctgcgtaata
    87541 aagatgccct ggtgttgcag agtgtttcaa ccatcgaact gccttatgaa gcagttaatc
    87601 ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag ttgcccaatc aattacaccc
    87661 tcatttcaaa cggcctggca aacctgaccg aaaaagtcga tcatgtcgtg gtagaaggga
    87721 ctggcggctg gcgtagtctg atgaatgatt tgcgtccact ctctgaatgg gtggtgcagg
    87781 aacaactgcc ggtgttgatg gttgtcggta ttcaggaagg ttgcattaac catgcactgc
    87841 taacagctca ggcgattgcc aacgacgggc tgccgctcat tggctgggtg gctaaccgaa
    87901 tcaacccagg actggcgcat tatgcggaaa tcattgatgt gctcggtaaa aaacttccgg
    87961 caccgctcat tggtgaactg ccttatctgc cgcgcgccga acagcgtgaa ctggggcaat
    88021 acatccgctt agctatgctg cgcagtgtgc tggcggtaga tagagtcacg gtgtaacgtc
    88081 cgcgagatta ccgacgcaat tacgcaggca atcaataaac cggggagtag ctgatactcc
    88141 ccggtcattt cacatatcat caacgtcgac ataatcggcg cgtgcgtggt tgccgccaac
    88201 agtgtcgcca tcccggtcaa tccgagtaaa agcgtaattt cttcgccatc agggaaccat
    88261 aatcccagac tacgaccata caacatgcca atggcaagac cgataaatag cgtcggtgta
    88321 aagacaccac cgggtgcgcc ggaaccgcta ctcgccagca cggcacacag tttacaaagg
    88381 aagatcccgg caatgatcat taacagtggc ggggcggtta aaaaggattg tacggtgcta
    88441 tagccgttgc cccacactgc aggtgtgaac agggaaagca gacccacgat caacccgcct
    88501 agtgccagtt gccagggcgg cgcaagtttg agactcacaa atccacgatg acaggcgttc
    88561 attaacgtta acaacagtgg tccgcacaga cctgccagca cacctgtact gataatcagc
    88621 gcatagtcac gagcctgaac cgtcactgag agttgtacgc tgtagagtaa cgcgtcgcta
    88681 tgattaatca gattgctaac cagcaatgcc acgacggcgg aaataatcac cgggccgaga
    88741 gaggccaaca tcatagtgcc aaacagcact tcggctataa ataaactgcc agcaagcggg
    88801 gcacgatagg ccgcagccat tcccgccgcg gccccacagg cgatccataa tttccactcc
    88861 tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa gggcagctaa aagaatcatc
    88921 gcaccttcgc gaccaattgc actgccgctg gttactacca gcagagaggc aagcgattta
    88981 accaggcttg ctgcgtaatc aaactgacca tcggtttgca acgcttccat gtaatcggtc
    89041 ggcgcatgag ggcgttgctg ggtaaatttc tgccagccca tcaacaacaa acccgccgcc
    89101 agtccgccga gcgccggagt tagcagccgt cgccaggggg aaaggtttgt cgctgcattg
    89161 accagactgc cggagtcatt attgaggaac aaccactcca gtagcagcat cgcatgacga
    89221 aacccggcaa cggcaaaggc cgcgagaata ccgacgactg ttgcgataag cagacggcgg
    89281 aacatagtgc gcagatccgg ataggtgtgt agatgatgca tgagcagaaa tgggcaatga
    89341 aacacgggcg actattttgc ggcaaaagag gccagctgca aaagcagtgc gtgctttaag
    89401 agacaaaaaa cgtgacactg taacgtattg ttgccgaatc gcgcgtaatc gtcaccatcc
    89461 ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa ttaacagttg
    89521 tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt agaaagggtg
    89581 ttctgctttt tcgataaaaa cgtcaagaaa acgtgttgac cagggaaaaa ggtgccatgc
    89641 cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag ccatcaacag
    89701 cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc
    89761 gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt cgcgatccag
    89821 ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca aacgctgcca
    89881 ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga
    89941 cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg caaccagagg
    90001 tgcggcttca gcactctctg gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac
    90061 agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat gtgcgggttg
    90121 ccccgcacca atttgttaac ctgcaatagc cattcctacc gtcatgtgca ggccataaaa
    90181 gagcacacgc ccgatcatct caccgccaag aatgaggatt aaccccagaa taagtccggc
    90241 aacgtgaggt tcacggcgac gtatgagcgg acaaagccag catcctagac ctgcgcataa
    90301 caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc tggcttgttg
    90361 cacggatgag tgaatagacg acagagacaa accttgcagc acaatcaccc cggcgcaagc
    90421 gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc
    90481 ccgcagaatt gcggcagcaa gtattggacc actcaacaat accgtcacga agaaagccag
    90541 cgtagtgtaa ccgttatgcc aggttggcac agtgtcgatt tgatacacgc aggtcatcat
    90601 ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc ccaacgcttg
    90661 cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa aaaatatgga
    90721 accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg agttaaacgc
    90781 ccgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca tcaacagcca
    90841 gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcagttttgg cggcgaacca
    90901 gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg taaagataac
    90961 cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta gccagataac
    91021 cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata ttgggttttg
    91081 tgaaatgcgc gcgcggcagt ggcgcgacgg cagcaagagt gccgtgcttc tggcgcagct
    91141 cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacacat atgggttgtt
    91201 tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct ttttcagcat
    91261 tgtactgtgg cgcgccgtac ggacaagcca tgtggcagta gcggcagccg atacaaacat
    91321 cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg gggcagactt
    91381 ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa gcgaacacgt
    91441 tctggtgcca gacgccatta tcttcctgcc agtcgccgcc agcatattca taaatacggc
    91501 ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg caggttttac
    91561 agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg ggctactcct
    91621 taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc cagcggtgaa
    91681 gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc accaaacata
    91741 tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat acgcggagtc
    91801 acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt atcgccatga
    91861 cggataccgc gagcctgagc atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc
    91921 tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttgtagtg gaagcccgtt
    91981 aactgcagtg gataggtttt ccgcagggga tcgtcccagc catcaaaacc tggggtatac
    92041 gcaggaaggg gatgaataat ttcatctttt ttcaattccc aggtatctgc aatcttcgcc
    92101 agtcgttcag aataaatttc gattttcccc gaaggtgttt tcaacgggtt tgcctgtgga
    92161 tcttcacgga atgcgcggaa agcgacgtag tgttcttccg ggcatttttt cttaaagatc
    92221 ccggtcgttt tcatctcctc gtagtcgggc atctcagggt tacgttcctt cgttttcgca
    92281 tggagatatt tgatccattc atgctgactg cgaccttcag taaaggtttg ataaacgtcc
    92341 ggtcctaagc gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt
    92401 gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag
    92461 ataaggtctt cttgctctgt tggcatcagg tcgggcaaca ggatatcgca atacttagcc
    92521 gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc
    92581 acctcattgg tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac
    92641 cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg
    92701 gtcatttccg taccatggtc gatggcatct gtccatgtaa agacggaaat ctgcgtttta
    92761 acaggattct cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca
    92821 cctgagttgc cgccgtttat gccgacgtta ccggtgagaa cggaaagcat ggcaatagcg
    92881 cgggatgttt gctcgccgtt ggaatgtcgt tgtggccccc aaccctgaca aatataagca
    92941 ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg
    93001 atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag
    93061 gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca
    93121 caatatttgt cgagaaatgg ctgatcgacc atgttttcac taatcagtac ccaggcaatc
    93181 gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg
    93241 gcagctgtgt cgttataacg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg
    93301 gcttgctcga cgtagtaagt gacaccacca ccgctcatcc gcgtttctgc cgggttattt
    93361 ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca
    93421 ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg
    93481 agaaaaccac cgcaagagtt catcagacgg tacgggacgt ttgagttggt gatgtttccg
    93541 ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc
    93601 cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta
    93661 ccttcaccgc gcttgccgac gcgcttcatg ggatatttca acctatcagg atgattcatc
    93721 cgtcggcgga tagagcgccc gcgtaaacac gcacgaacct gatgattacc gtagacgtcg
    93781 tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtaac
    93841 agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgctttctc ttctaccggt
    93901 gcctctgccg ctcggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgca
    93961 agactgccaa gtgcggaggt tttcatcaga cttctacggc tgatttcagc cttcatgagc
    94021 gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg
    94081 ccagaattta tggttgtagt gggttatatt ttttcgatct cgaccagatt cgtgtgctgc
    94141 gggtttccct tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca
    94201 tggtcgattt tatcgccaga catattggcc tcgtgccagg ctccctggcc catagcgcta
    94261 accccaggga gaatgcgtgg tgtcactttg gctggtagcc gaacttcgcc acgatggtta
    94321 tacacccgca ccatatcgcc gttggcaatc ccacgtttct gtgcgtctat agggttgatc
    94381 cacacctcct gacggcaggc agccttcagg acatcaatgt tgccgtaggt cgagtgagta
    94441 cgggatttgt aatggaaacc aaacagttgc agtggaaagg tactacgttc aggggagtcc
    94501 cagccttcaa aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt
    94561 tcccaggtac gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggt
    94621 gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatgacca
    94681 ttgggatctt tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc
    94741 ggatctttgg caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct
    94801 tcagtgaact tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag
    94861 taaatcggtt tgcgttcgaa tttttcgctg gtgacaggct ggaggaaaat gagatatccc
    94921 atgttaccgg cgtagtcgtt aggaataata tcttcctgct caacggtcat caggtctggc
    94981 agcagaatgt cggcatattt tgccgatgag gtcataaagt tttcgatgac cacaatcatt
    95041 tcgcatttcg attcgtcctg cagaatttca tgcgttttgt tgatgtcaga atgctgatta
    95101 accagggtat ttcccgcgta gttccagatg aacttaatgg gcacatccag tttatctttg
    95161 ccccggacac cgtcgcggat tgccgtcatt tgtgggccat gatcgatagc atctgtccag
    95221 ctgaagcagg aaattgacgt tttgaccgga ttatccagca ccggcaggcg ctctatggta
    95281 atggtatagg tcgattcacg cgcgccacta tttccgccgc tgatgccgac attgcccgtc
    95341 agaataggca acatagcaat agcgcgtgcg gtcagctcgc cgtttgcctg gcgttgcggc
    95401 ccccagccct ggcagatata agcgggtttt gctgtgccaa tttcacgcgc cagtttgatg
    95461 atacggtcca ccgggatacc ggtaatttgc gaagcccact gcggcgtttt cgctgtttta
    95521 tcgtcacctt caccaagaat ataggcttta tagtgaccat ttttgggtgc atctgcgggt
    95581 aaggtttttt cgtcatagcc gacgcagtat ttatcgagaa aaggttgatc aacgagattt
    95641 tcgttaatca atacccaggc aatacccgca accagcgcgg catcggtgcc cgggcgaata
    95701 ggaagccatt catcttcacg accagcagcc gtatcggtat atcgcggatc gataacaatc
    95761 atttttgcgt tcgatttttc gcgggatttt tcaagaagat aagtgatgcc accgccgctc
    95821 atgcgggttt ctgccgggtt gttaccaaac atcacgacca gcttgctgtt ttcaatatcc
    95881 gtggtgctgt tgccatcatt actgccgtag gtgtagggca tggcacagga aatttgcgca
    95941 gtgctgtagg agccatactg gttgagtgaa ccgccgtagc agttcatcag gcgtttgacc
    96001 gccgaggctg atggcgaaga gcgggttata ttgccgccaa cgatccccga agagtactga
    96061 atatatacag cctcattgcc atattgttcg acggttttct tcaggctact ggcgatagta
    96121 tccagggctt catcccagct aatccgttcg aatttgcctt caccgcgttt acccacgcgt
    96181 ttcattgggt aattcaagcg atcgggatga ttaatacgcc ggcggatgga gcgaccgcgc
    96241 aaacaggcgc gtacctgatg gttgccatac tcatcgctgc cggtgttgtc agtttccacc
    96301 caggccactt cattatcttt aacatgtaga cgaagtgcac agcggctacc acagttgacg
    96361 gaacaggcac cccagaccac ttgttcgctg gcctgttgta ctgctgccgc tgcactgcgc
    96421 agggtgaacg gcaaagaaaa accgcctgca gccagcgcca gagaacctat cgcggtagat
    96481 ttaacgagtg ttctgcggct gatgcccacc attcgttcat ttttggacat aactcactcc
    96541 ctgttcttta tcgttatata aaagtttata tattgaatat ttagcgcgct aacaatagag
    96601 ggggtctacc cattttgggt taataattat taatccatat caatagaagg gtatgagtaa
    96661 tatggtggga ttatgttgta tgtccaaatc gccggatgtg tcgtatccgg cgtgtagtcg
    96721 ataatgtatt actgcggttc ggcaggcgcg ccatcctggg tagactgcgc gggagcagag
    96781 acgttaccgc tggtggtgcg ggtgtagaga attttatgcg tatcattagc gcaatgcccg
    96841 acgacctggg aatcaggctg atcaacttgg tcattgggta caatacttaa cgtgaagctg
    96901 ctttcgggta cgccattatt gataatgcgc tgtgatatat cgctttgtat gcgctcacag
    96961 gatcccggcg cggcgagtac cgcgggtgag gcgagggcga gcagaagcgc ggcataacag
    97021 gttgagagtt tcatcataag ctcctaacgc gaagataact tctttaagca tagcatttca
    97081 cgtgtaaagt actgtatttg ctactatgat tgagaatcat ctctactctc tggtgactga
    97141 tgtgaaatac aaattactgc catgcttact cgcgatatta ctcacaggat gtgaccgcac
    97201 agaggtaaca ctttcattta ctcctgagat ggccagtttc tctaatgaat tcgattttga
    97261 tccgctgcgt ggtccggtaa aagatttcac tcagacatta atggatgagc aaggtgaagt
    97321 gacgaaacgt gtttcgggga ctttgtcgga agaaggctgt tttgattcac tcgaattact
    97381 ggatctggaa aataataccg tggtcgctct ggtactggac gccaattatt accgtgatgc
    97441 cgagacgctg gagaagagag tacgtttaca gggaaaatgc cagctagcag aattaccttc
    97501 tgccggggtg agttgggaaa ccgatgataa tggcttcgtg attaaagcca gcagcaaaca
    97561 aatgcagatg gaatatcgct atgatgatca gggttatcca ctgggtaaaa ccacgaaaag
    97621 taacgacaaa acattatctg tcagcgccac gccatcaacg gacccgatca aaaaattaga
    97681 ttacacagcg gttactttac tgaataatca acgagttggt aatgtaaaac agagctgtga
    97741 atatgacagt cacgccaatc cggtgggctg tcagctgatc attgttgatg aaggagtaaa
    97801 acccgccgtc gaacgggttt acaccatcaa aaatacgatc gattattatt aatgctattg
    97861 tgctgtcggc ttcaggagag tctgacccgg tgttttgtgc tctgccagat actgatgctg
    97921 gaatatacac atgcgaatgg cattacgata ttgaccatta ataaagaact cgtgcatcaa
    97981 ttcaccttca accgaaaagc caagcttgcg gtaaatgtga atcgcttttt cattctcttt
    98041 atcaacgatc agatacagct tatagagatt gagaacggtg aagccatagt ccattgctaa
    98101 tttggcggca cgggttgcca gacctttccc ctgatactcc ggggagataa ttatctgaaa
    98161 ttctgcgcgg cgatgtacat ggttaatttc caccaactcc accagaccgg ctttttcgcc
    98221 gtcacattcc accacaaagc gtcgttcgct ctgatcgtga atatgcttat catacagatc
    98281 agagagttca acaaaggctt cgtagggttc ctcaaaccag taacgcatca cactggcgtt
    98341 attgtcgagt tgatgtacat agcgtaaatc ttcacgctcc agcgggcgta gcttaacact
    98401 gtgggcgctt ggcataacgt gtccttacat tccttaaatc aataacaggt tagggggtaa
    98461 taacgcggcc agttcgacgg tccaggcagc gcaaagtatt tggctcccag taggcattga
    98521 tgttggcgct ttgctcgcat ttatcgcggt tatcaaaagc ggcgtcggct ttatcccact
    98581 ctttttcagt gcgtttattc actttctggc gcagattgcc cgtgtcattc cattgctctt
    98641 tttccatagc ggcgtgctgg cggctttgtg cactgtcgcc agactcaatc accagtttgt
    98701 tagtttcggc atgaacagtt gtgctcaatg ccagtgcgca aggcagcaga aaagcgagca
    98761 ggtcgattcg tttgctgaga gtgattttca taattcattc cctgtatgaa tgattaaaga
    98821 tgattctaca ccatccactg cgaacgcaaa acgtaccagg agggtgttta tattgatgat
    98881 attatgtcgc cctataacta tacatgatgt caataagtga caaagatgat taaaacaacg
    98941 ttactatttt ttgctactgc gctgtgtgaa attattggat gctttctgcc ctggttgtgg
    99001 ttaaaacgaa acgccagtat ctggctgttg cttccggcgg ggatttcact ggcgctgttt
    99061 gtctggttgt taacgttgca tcctgcggcg agtgggcgtg tttacgcggc ttatggtggc
    99121 gtttatgtct gcacggcgtt gatgtggctg cgcgttgtgg atggcgtgaa actgactctt
    99181 tatgactgga cggggccgtt gattgcgctt tgcggcatgt tgatcattgt tgtgggctgg
    99241 gggcgcacgt aggaacataa atccatttta tcaataagat acgaggaagt gtcagctgac
    99301 aaaaggtatt ctatttcatc ttttgtcaac cattcacagc gcaaatatac gccttttttt
    99361 gtgatcactc cggctttttt cgatcttcat acttatatgg tagtagctca gttgcgtaga
    99421 tttcatgcat cacgacaagc gatgcaagga atcgaacatg aagatcgtaa aggctgaagt
    99481 ttttgttacc tgtccggggc gtaatttcgt cacattaaaa atcaccactg aggacggtat
    99541 tacaccataa atttttggct tgctgatttc tgcacgcatc gataagacgg atgaattcct
    99601 cccgcgaaag aggatccgga atggttcttg attcctttaa tggcgagatc cccttaaacg
    99661 ggttatctgc caggtaaccg ttatcaacac caaactggaa cacggcgtta agatttgtca
    99721 tgtaattatt tacagttaca gccgatctcc ctggttgtgt aacaatatag ttacttttgg
    99781 ggatctggta tccagtcagt agctctttac gaacctccag taatttttct ttattaatcg
    99841 atgaggcaag atttttttca ccgattatgc tcaggatatt tttgatgacg gcacggtatg
    99901 tgttgagtga tgtttttgcg acttcagttt ctttcagtgc cagaaatttt tcagccagtt
    99961 cttttatggt taaatcttgt cgggcctcac caaatttttc cagattgcgt gaggagggaa
   100021 actgttttgc atagtcgaaa acaccagttt ttattgcgta acaaacagag gcgcgtagct
   100081 cacctgcaac gcgcctgttt tttgctgtgt caggaacccc aggttttccc tgactcttac
   100141 gcctttataa acaaaccaga tacgtaattt ccctccatgg ttttccacgc ctgtcggata
   100201 tttcatttca acttctctca ttagttagtg tggcttttag tcaagtaaga tgacgtcttg
   100261 gtcttgctga tgtctggcgc tcaatccagc gatcaatttc ttccaggttg taaaagcatg
   100321 gactgttatc ccatggcata ccgtcatgag cgacatgctt atattccctt ccttccataa
   100381 acgatttttc ccgggccttt tttaacgtac ctttttttat tcctttcagc gcaattaact
   100441 gctcttcgga tacccatttg ccgggagaga caatcatgat tacttcgctc atcgatttct
   100501 ttatctctta catcagacga gcgccggttg cagaatacca gtcacaaccg gcgacagttg
   100561 aacattaaga atcagcctga ctcgggatca gtttttgcca gatagctgaa acgtattttg
   100621 cctggtaacg ggcgtcatca agtgcattat ggcgctcacc ttcgaatgga atagccgttc
   100681 tggcatcgaa gtctatggct ttccccagct caacgattgt gcgtacatcg cgatcgttgt
   100741 agtaacgcca cgggcagggg ctcccctgcc gttcgtatga acggcgcaaa atcgtgttgt
   100801 cgaagttggc tccatttccc cagacatgaa caaaaaattc accggagttt tcgtcgataa
   100861 attcccgcaa ttgtaacagt gcatcatcta acgggatttc atcggtcata atggcagatt
   100921 gcgcttcgcg tgattgttta agccaccatt tcatggtgtc ccgatcaatg actccgccag
   100981 cagtatccag atcgatagtc ttactaaatt ccggtcccat atctccggtt tgcggatcga
   101041 aaaatattgc acctattgag ataatcgggg catcgggatt ttttcccatg gtttcaagat
   101101 cgatcatcag atgaatcccc gctctgctgg tggatgtgag attatgatga ccgttcgcct
   101161 taattaaggg atctgacgcc tcgccagttt cactatcgct ggcatgatgc tgattgccgc
   101221 cagtgttctc cttgtgctga tgtgcagtac cttccatttc ctccggatca ttttcctgaa
   101281 cttcaggctg attctctcca tcgaatattt cctggtatgt tgcgtcaccc atcaccgcac
   101341 cacaatcagg gcagttgccg ccaccgctct gaccgcaggc ggtgcagatc ttttccggtt
   101401 cctgttgcac tactggttca ggttgtttcg tttctggctc gttttgttgc gtatttgggc
   101461 tgttttgttc cgctttctgg tcgttctgtt ccgtttcttg ctggttctgg ttcacagaat
   101521 cgcgggtctg gatcccctta acccatttcg gatcattcgg gtcgctaatc ccttcaacaa
   101581 attcaccacg tgatactgca agcagttcct
//
LOCUS       DAZFTN010000003       100375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFTN010000003 DAZFTN010000000
VERSION     DAZFTN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            57..575
                     /gene="yfgH"
                     /locus_tag="ACHKV2_000224"
     CDS             57..575
                     /gene="yfgH"
                     /locus_tag="ACHKV2_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6938188.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMMNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            591..1130
                     /gene="yfgI"
                     /locus_tag="ACHKV2_000225"
     CDS             591..1130
                     /gene="yfgI"
                     /locus_tag="ACHKV2_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO6938189.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1223..2800)
                     /gene="guaA"
                     /locus_tag="ACHKV2_000226"
     CDS             complement(1223..2800)
                     /gene="guaA"
                     /locus_tag="ACHKV2_000226"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO6938190.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2869..4335)
                     /gene="guaB"
                     /locus_tag="ACHKV2_000227"
     CDS             complement(2869..4335)
                     /gene="guaB"
                     /locus_tag="ACHKV2_000227"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO6938191.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4497..5867
                     /gene="xseA"
                     /locus_tag="ACHKV2_000228"
     CDS             4497..5867
                     /gene="xseA"
                     /locus_tag="ACHKV2_000228"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO6938192.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5864..6079)
                     /gene="yfgJ"
                     /locus_tag="ACHKV2_000229"
     CDS             complement(5864..6079)
                     /gene="yfgJ"
                     /locus_tag="ACHKV2_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6938193.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6148..7620)
                     /gene="der"
                     /locus_tag="ACHKV2_000230"
     CDS             complement(6148..7620)
                     /gene="der"
                     /locus_tag="ACHKV2_000230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO6938194.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7738..8916)
                     /gene="bamB"
                     /locus_tag="ACHKV2_000231"
     CDS             complement(7738..8916)
                     /gene="bamB"
                     /locus_tag="ACHKV2_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO6938195.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8927..9547)
                     /gene="yfgM"
                     /locus_tag="ACHKV2_000232"
     CDS             complement(8927..9547)
                     /gene="yfgM"
                     /locus_tag="ACHKV2_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO6938196.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9565..10839)
                     /gene="hisS"
                     /locus_tag="ACHKV2_000233"
     CDS             complement(9565..10839)
                     /gene="hisS"
                     /locus_tag="ACHKV2_000233"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO6938197.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10950..12068)
                     /gene="ispG"
                     /locus_tag="ACHKV2_000234"
                     /gene_synonym="gcpE"
     CDS             complement(10950..12068)
                     /gene="ispG"
                     /locus_tag="ACHKV2_000234"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO6938198.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12095..13108)
                     /gene="rodZ"
                     /locus_tag="ACHKV2_000235"
     CDS             complement(12095..13108)
                     /gene="rodZ"
                     /locus_tag="ACHKV2_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO6938199.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13393..14547)
                     /gene="rlmN"
                     /locus_tag="ACHKV2_000236"
     CDS             complement(13393..14547)
                     /gene="rlmN"
                     /locus_tag="ACHKV2_000236"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO6938200.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14697..15128)
                     /gene="ndk"
                     /locus_tag="ACHKV2_000237"
     CDS             complement(14697..15128)
                     /gene="ndk"
                     /locus_tag="ACHKV2_000237"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO6938201.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15269..16123)
                     /locus_tag="ACHKV2_000238"
     CDS             complement(15269..16123)
                     /locus_tag="ACHKV2_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6938202.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16123..16944)
                     /locus_tag="ACHKV2_000239"
     CDS             complement(16123..16944)
                     /locus_tag="ACHKV2_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO6938203.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16937..17566)
                     /locus_tag="ACHKV2_000240"
     CDS             complement(16937..17566)
                     /locus_tag="ACHKV2_000240"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO6938204.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17563..19944)
                     /locus_tag="ACHKV2_000241"
     CDS             complement(17563..19944)
                     /locus_tag="ACHKV2_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO6938205.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEIIPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
     gene            complement(20108..22420)
                     /gene="pbpC"
                     /locus_tag="ACHKV2_000242"
     CDS             complement(20108..22420)
                     /gene="pbpC"
                     /locus_tag="ACHKV2_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO6938206.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(22421..27382)
                     /gene="yfhM"
                     /locus_tag="ACHKV2_000243"
     CDS             complement(22421..27382)
                     /gene="yfhM"
                     /locus_tag="ACHKV2_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO6938207.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            27589..28434
                     /gene="sseA"
                     /locus_tag="ACHKV2_000244"
     CDS             27589..28434
                     /gene="sseA"
                     /locus_tag="ACHKV2_000244"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO6938208.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            28777..28902
                     /gene="timP"
                     /locus_tag="ACHKV2_000245"
     CDS             28777..28902
                     /gene="timP"
                     /locus_tag="ACHKV2_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO6938209.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(28934..29710)
                     /gene="sseB"
                     /locus_tag="ACHKV2_000246"
     CDS             complement(28934..29710)
                     /gene="sseB"
                     /locus_tag="ACHKV2_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO6938210.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(29853..31136)
                     /gene="pepB"
                     /locus_tag="ACHKV2_000247"
     CDS             complement(29853..31136)
                     /gene="pepB"
                     /locus_tag="ACHKV2_000247"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO6938211.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(31314..31514)
                     /gene="iscX"
                     /locus_tag="ACHKV2_000248"
     CDS             complement(31314..31514)
                     /gene="iscX"
                     /locus_tag="ACHKV2_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO6938212.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(31526..31861)
                     /gene="fdx"
                     /locus_tag="ACHKV2_000249"
     CDS             complement(31526..31861)
                     /gene="fdx"
                     /locus_tag="ACHKV2_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO6938213.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(31863..33713)
                     /gene="hscA"
                     /locus_tag="ACHKV2_000250"
     CDS             complement(31863..33713)
                     /gene="hscA"
                     /locus_tag="ACHKV2_000250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO6938214.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(33730..34245)
                     /gene="hscB"
                     /locus_tag="ACHKV2_000251"
     CDS             complement(33730..34245)
                     /gene="hscB"
                     /locus_tag="ACHKV2_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO6938215.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(34341..34664)
                     /gene="iscA"
                     /locus_tag="ACHKV2_000252"
     CDS             complement(34341..34664)
                     /gene="iscA"
                     /locus_tag="ACHKV2_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO6938216.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(34681..35067)
                     /gene="iscU"
                     /locus_tag="ACHKV2_000253"
     CDS             complement(34681..35067)
                     /gene="iscU"
                     /locus_tag="ACHKV2_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO6938217.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(35095..36309)
                     /gene="iscS"
                     /locus_tag="ACHKV2_000254"
     CDS             complement(35095..36309)
                     /gene="iscS"
                     /locus_tag="ACHKV2_000254"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO6938218.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(36421..36909)
                     /gene="iscR"
                     /locus_tag="ACHKV2_000255"
     CDS             complement(36421..36909)
                     /gene="iscR"
                     /locus_tag="ACHKV2_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO6938219.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(37210..37947)
                     /gene="trmJ"
                     /locus_tag="ACHKV2_000256"
     CDS             complement(37210..37947)
                     /gene="trmJ"
                     /locus_tag="ACHKV2_000256"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO6938220.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            38066..38869
                     /gene="suhB"
                     /locus_tag="ACHKV2_000257"
     CDS             38066..38869
                     /gene="suhB"
                     /locus_tag="ACHKV2_000257"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO6938221.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            39014..39868
                     /gene="yfhR"
                     /locus_tag="ACHKV2_000258"
     CDS             39014..39868
                     /gene="yfhR"
                     /locus_tag="ACHKV2_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6938222.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            40059..41339
                     /gene="csiE"
                     /locus_tag="ACHKV2_000259"
     CDS             40059..41339
                     /gene="csiE"
                     /locus_tag="ACHKV2_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO6938223.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(41331..42470)
                     /gene="hcaT"
                     /locus_tag="ACHKV2_000260"
     CDS             complement(41331..42470)
                     /gene="hcaT"
                     /locus_tag="ACHKV2_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO6938224.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(42630..43520)
                     /gene="hcaR"
                     /locus_tag="ACHKV2_000261"
     CDS             complement(42630..43520)
                     /gene="hcaR"
                     /locus_tag="ACHKV2_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO6938225.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            43656..45017
                     /gene="hcaE"
                     /locus_tag="ACHKV2_000262"
     CDS             43656..45017
                     /gene="hcaE"
                     /locus_tag="ACHKV2_000262"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO6938226.1"
                     /translation="MTIPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            45014..45532
                     /gene="hcaF"
                     /locus_tag="ACHKV2_000263"
     CDS             45014..45532
                     /gene="hcaF"
                     /locus_tag="ACHKV2_000263"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO6938227.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            45532..45852
                     /gene="hcaC"
                     /locus_tag="ACHKV2_000264"
     CDS             45532..45852
                     /gene="hcaC"
                     /locus_tag="ACHKV2_000264"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO6938228.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            45849..46661
                     /gene="hcaB"
                     /locus_tag="ACHKV2_000265"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             45849..46661
                     /gene="hcaB"
                     /locus_tag="ACHKV2_000265"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO6938229.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            46671..47873
                     /gene="hcaD"
                     /locus_tag="ACHKV2_000266"
     CDS             46671..47873
                     /gene="hcaD"
                     /locus_tag="ACHKV2_000266"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO6938230.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            47970..48392
                     /gene="yphA"
                     /locus_tag="ACHKV2_000267"
     CDS             47970..48392
                     /gene="yphA"
                     /locus_tag="ACHKV2_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6938231.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(48440..49312)
                     /gene="yphB"
                     /locus_tag="ACHKV2_000268"
     CDS             complement(48440..49312)
                     /gene="yphB"
                     /locus_tag="ACHKV2_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6938232.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(49324..50418)
                     /gene="yphC"
                     /locus_tag="ACHKV2_000269"
                     /pseudo
     CDS             complement(49324..50418)
                     /gene="yphC"
                     /locus_tag="ACHKV2_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(50451..51449)
                     /gene="yphD"
                     /locus_tag="ACHKV2_000270"
     CDS             complement(50451..51449)
                     /gene="yphD"
                     /locus_tag="ACHKV2_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6938233.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(51474..52985)
                     /gene="yphE"
                     /locus_tag="ACHKV2_000271"
     CDS             complement(51474..52985)
                     /gene="yphE"
                     /locus_tag="ACHKV2_000271"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6938234.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(53008..53991)
                     /gene="yphF"
                     /locus_tag="ACHKV2_000272"
     CDS             complement(53008..53991)
                     /gene="yphF"
                     /locus_tag="ACHKV2_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6938235.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(54088..57369)
                     /gene="yphG"
                     /locus_tag="ACHKV2_000273"
     CDS             complement(54088..57369)
                     /gene="yphG"
                     /locus_tag="ACHKV2_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO6938236.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            57487..58680
                     /gene="yphH"
                     /locus_tag="ACHKV2_000274"
     CDS             57487..58680
                     /gene="yphH"
                     /locus_tag="ACHKV2_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6938237.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(58743..59996)
                     /gene="glyA"
                     /locus_tag="ACHKV2_000275"
     CDS             complement(58743..59996)
                     /gene="glyA"
                     /locus_tag="ACHKV2_000275"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO6938238.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            60324..61514
                     /gene="hmpA"
                     /locus_tag="ACHKV2_000276"
     CDS             60324..61514
                     /gene="hmpA"
                     /locus_tag="ACHKV2_000276"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO6938239.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(61559..61897)
                     /gene="glnB"
                     /locus_tag="ACHKV2_000277"
     CDS             complement(61559..61897)
                     /gene="glnB"
                     /locus_tag="ACHKV2_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO6938240.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(61958..63292)
                     /gene="glrR"
                     /locus_tag="ACHKV2_000278"
     CDS             complement(61958..63292)
                     /gene="glrR"
                     /locus_tag="ACHKV2_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO6938241.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(63282..63995)
                     /gene="qseG"
                     /locus_tag="ACHKV2_000279"
     CDS             complement(63282..63995)
                     /gene="qseG"
                     /locus_tag="ACHKV2_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO6938242.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(64160..65587)
                     /gene="qseE"
                     /locus_tag="ACHKV2_000280"
     CDS             complement(64160..65587)
                     /gene="qseE"
                     /locus_tag="ACHKV2_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO6938243.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(66163..70050)
                     /gene="purL"
                     /locus_tag="ACHKV2_000281"
                     /gene_synonym="purI"
     CDS             complement(66163..70050)
                     /gene="purL"
                     /locus_tag="ACHKV2_000281"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO6938244.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            70307..71863
                     /gene="mltF"
                     /locus_tag="ACHKV2_000282"
     CDS             70307..71863
                     /gene="mltF"
                     /locus_tag="ACHKV2_000282"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO6938245.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(71860..72363)
                     /gene="tadA"
                     /locus_tag="ACHKV2_000283"
     CDS             complement(71860..72363)
                     /gene="tadA"
                     /locus_tag="ACHKV2_000283"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO6938246.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(72421..73056)
                     /gene="yfhb"
                     /locus_tag="ACHKV2_000284"
     CDS             complement(72421..73056)
                     /gene="yfhb"
                     /locus_tag="ACHKV2_000284"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO6938247.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            73265..74113
                     /gene="yfhH"
                     /locus_tag="ACHKV2_000285"
     CDS             73265..74113
                     /gene="yfhH"
                     /locus_tag="ACHKV2_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6938248.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            74169..74429
                     /gene="yfhL"
                     /locus_tag="ACHKV2_000286"
     CDS             74169..74429
                     /gene="yfhL"
                     /locus_tag="ACHKV2_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO6938249.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(74623..74703)
                     /gene="shoB"
                     /locus_tag="ACHKV2_000287"
     CDS             complement(74623..74703)
                     /gene="shoB"
                     /locus_tag="ACHKV2_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO6938250.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(75125..75505)
                     /gene="acpS"
                     /locus_tag="ACHKV2_000288"
     CDS             complement(75125..75505)
                     /gene="acpS"
                     /locus_tag="ACHKV2_000288"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6938251.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(75505..76236)
                     /gene="pdxJ"
                     /locus_tag="ACHKV2_000289"
     CDS             complement(75505..76236)
                     /gene="pdxJ"
                     /locus_tag="ACHKV2_000289"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO6938252.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(76248..76976)
                     /gene="recO"
                     /locus_tag="ACHKV2_000290"
     CDS             complement(76248..76976)
                     /gene="recO"
                     /locus_tag="ACHKV2_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO6938253.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(76988..77893)
                     /gene="era"
                     /locus_tag="ACHKV2_000291"
     CDS             complement(76988..77893)
                     /gene="era"
                     /locus_tag="ACHKV2_000291"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO6938254.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(77890..78570)
                     /gene="rnc"
                     /locus_tag="ACHKV2_000292"
     CDS             complement(77890..78570)
                     /gene="rnc"
                     /locus_tag="ACHKV2_000292"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO6938255.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(78843..79817)
                     /gene="lepB"
                     /locus_tag="ACHKV2_000293"
     CDS             complement(78843..79817)
                     /gene="lepB"
                     /locus_tag="ACHKV2_000293"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO6938256.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(79833..81632)
                     /gene="lepA"
                     /locus_tag="ACHKV2_000294"
     CDS             complement(79833..81632)
                     /gene="lepA"
                     /locus_tag="ACHKV2_000294"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO6938257.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(81830..82309)
                     /gene="rseC"
                     /locus_tag="ACHKV2_000295"
     CDS             complement(81830..82309)
                     /gene="rseC"
                     /locus_tag="ACHKV2_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO6938258.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(82306..83262)
                     /gene="rseB"
                     /locus_tag="ACHKV2_000296"
     CDS             complement(82306..83262)
                     /gene="rseB"
                     /locus_tag="ACHKV2_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO6938259.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(83262..83900)
                     /gene="rseA"
                     /locus_tag="ACHKV2_000297"
     CDS             complement(83262..83900)
                     /gene="rseA"
                     /locus_tag="ACHKV2_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO6938260.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(83933..84508)
                     /gene="rpoE"
                     /locus_tag="ACHKV2_000298"
     CDS             complement(83933..84508)
                     /gene="rpoE"
                     /locus_tag="ACHKV2_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO6938261.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(84505..84660)
                     /gene="rseD"
                     /locus_tag="ACHKV2_000299"
     CDS             complement(84505..84660)
                     /gene="rseD"
                     /locus_tag="ACHKV2_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO6938262.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            84916..86538
                     /gene="nadB"
                     /locus_tag="ACHKV2_000300"
     CDS             84916..86538
                     /gene="nadB"
                     /locus_tag="ACHKV2_000300"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO6938263.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(86523..87260)
                     /gene="trmN"
                     /locus_tag="ACHKV2_000301"
     CDS             complement(86523..87260)
                     /gene="trmN"
                     /locus_tag="ACHKV2_000301"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO6938264.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            87392..88726
                     /gene="srmB"
                     /locus_tag="ACHKV2_000302"
     CDS             87392..88726
                     /gene="srmB"
                     /locus_tag="ACHKV2_000302"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO6938265.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(88759..89640)
                     /gene="yfiE"
                     /locus_tag="ACHKV2_000303"
     CDS             complement(88759..89640)
                     /gene="yfiE"
                     /locus_tag="ACHKV2_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6938266.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            89743..90330
                     /gene="eamB"
                     /locus_tag="ACHKV2_000304"
     CDS             89743..90330
                     /gene="eamB"
                     /locus_tag="ACHKV2_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO6938267.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(90386..90769)
                     /gene="grcA"
                     /locus_tag="ACHKV2_000305"
     CDS             complement(90386..90769)
                     /gene="grcA"
                     /locus_tag="ACHKV2_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO6938268.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            91074..91763
                     /gene="ung"
                     /locus_tag="ACHKV2_000306"
     CDS             91074..91763
                     /gene="ung"
                     /locus_tag="ACHKV2_000306"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO6938269.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(91811..92848)
                     /gene="yfiF"
                     /locus_tag="ACHKV2_000307"
     CDS             complement(91811..92848)
                     /gene="yfiF"
                     /locus_tag="ACHKV2_000307"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6938270.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            93055..93474
                     /gene="trxC"
                     /locus_tag="ACHKV2_000308"
     CDS             93055..93474
                     /gene="trxC"
                     /locus_tag="ACHKV2_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO6938271.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            93543..94241
                     /gene="tapT"
                     /locus_tag="ACHKV2_000309"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             93543..94241
                     /gene="tapT"
                     /locus_tag="ACHKV2_000309"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO6938272.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            94273..96933
                     /gene="pat"
                     /locus_tag="ACHKV2_000310"
     CDS             94273..96933
                     /gene="pat"
                     /locus_tag="ACHKV2_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO6938273.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            97047..98402
                     /gene="pssA"
                     /locus_tag="ACHKV2_000311"
     CDS             97047..98402
                     /gene="pssA"
                     /locus_tag="ACHKV2_000311"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO6938274.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDYVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            98448..98771
                     /gene="yfiM"
                     /locus_tag="ACHKV2_000312"
     CDS             98448..98771
                     /gene="yfiM"
                     /locus_tag="ACHKV2_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO6938275.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(98768..100066)
                     /gene="kgtP"
                     /locus_tag="ACHKV2_000313"
     CDS             complement(98768..100066)
                     /gene="kgtP"
                     /locus_tag="ACHKV2_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO6938276.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(100183..>100375)
                     /locus_tag="ACHKV2_000314"
     CDS             complement(100183..>100375)
                     /locus_tag="ACHKV2_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938277.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT        24093 a        27299 c        25082 g        23901 t
ORIGIN      
        1 tcctcttttg atgatcttaa atgtcttatt tttcgtaatg tgtataacaa ggaatagtga
       61 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat
      121 gccagtcaaa tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca
      181 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      241 actcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg
      301 gtgttatcgg ccacaacgtc gggtctggca gcaattccgg aacgacggca ggtgcagttg
      361 gcggcggagc tgtaggcgcg gcagcgggtt ctatgatgaa tgataaaacc ttagtggaag
      421 gtgtttcttt aacgtataag gaaggcacca aagtgtatac ctctactcag gtgggtaaag
      481 agtgccagtt tacgacaggt ttagccgttg ttattaccac gacgtataac gaaacgcgta
      541 ttcagccaaa taccaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag
      601 tttttctttg cgccatctta gcctccttaa gctatccggc tatcgcctca tcattgcagg
      661 atcaactctc tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc
      721 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      781 atgcccaggc tgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc
      841 aggataaact ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg
      901 agctacgcag cttagagatt cagaaacaaa aactggcgct ggcaaaagaa gaagcccgcg
      961 ttaagcgaga aaacgaattt atcgatcagg agctgaagca caaagccgcg caaactgatg
     1021 tcgtgcaatc tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga
     1081 aaagcgtggg caaagcagaa gagaataaat cggacagctg gtttaattaa tcgatgttag
     1141 taacttcaag cctataattc ttgaagataa aaaaccctct gtagtaacag agggttttgt
     1201 tcattcatag tgcagggtca aatcattccc actcaatggt agctggtggc ttgccgctga
     1261 tgtcatacac cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac
     1321 cgaggaaatc gtacggcaga tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag
     1381 cacgcagaga gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta
     1441 ctggcaggaa gacggtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt
     1501 cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac
     1561 ccagaacacg aacgccaagg cctggtcccg ggaacgggtg acggtacagc atgtcgtacg
     1621 gcaggcccag ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt
     1681 caaccaggcc catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga
     1741 cgtgtgcttt accggttgca gacgccgcag attcgataac gtcagggtag atggtgccct
     1801 gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa
     1861 cgcgaccgat gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga
     1921 agcgatcttc tgccggtacg tgaacaatgt taagaccaaa gtgatcgcca aacatatcca
     1981 gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt
     2041 ttttaccgat agcgcggtgc agcagcattg cggttacgga ggaatccaca ccaccagaga
     2101 ggccgaggat gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga
     2161 taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca
     2221 gcatgcgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc
     2281 gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga
     2341 agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt
     2401 taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt
     2461 caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca
     2521 ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat
     2581 actgcggcgc acgcgggctg ttttcttcag tagtactttc cgggccgccg gaaagaataa
     2641 tgccgcttgg attgaagtca cgaatttgtg cttctgtcac atcccatgcc cacagttcgc
     2701 agtaaacacc cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt
     2761 ccagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa
     2821 gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag
     2881 acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc
     2941 cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc
     3001 ggtcaaaccc atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg
     3061 acctttataa gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc
     3121 gctctggaaa taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg
     3181 gtaagatttg taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc
     3241 cgccagcatg gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc
     3301 gccggagaag cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc
     3361 aactgcgtca gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt
     3421 acagatagaa ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccagagc
     3481 gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc
     3541 acgggtttca cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag
     3601 cagaacgtca acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc
     3661 gccaactgca gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg
     3721 ttccgctttc tggaaatctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac
     3781 caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc
     3841 accttcacgc acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt
     3901 caggtcggta acaaaacgca cgtcacgacc ggtgataatc cccaccagtt cgttttcttc
     3961 ggtaacgacc ggatagcccg caaaaccgtt acgctcggtc agttctttca cttcgcgcag
     4021 cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac
     4081 acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc
     4141 gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga
     4201 aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt
     4261 cggcagaacg gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt
     4321 agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat
     4381 tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga
     4441 agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt
     4501 taccttctca atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc
     4561 tgcttgagca tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac
     4621 cggcttccgg tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga
     4681 tgttccgcaa cagcaaccgc cgggtgacct tccgcccaca gcatgggcaa caagttttag
     4741 ttcgcgccaa tattacgctc tacgagccgc gcggcgacta ccagataatc gttgagagta
     4801 tgcagccggc cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc
     4861 aggctgaagg attgttcgat ctgcaataca aaaaaccact tccctcccct gcgcattgcg
     4921 ttggtgtgat cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac
     4981 gtcgcgatcc ttctctgccg gtgatcatct accccaccgc cgttcagggc gatgacgcgc
     5041 cggggcaaat tgttcgcgcc attgaactgg cgaatcagtg caatgagtgc gacgtgttga
     5101 tcgttgggcg cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag
     5161 cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg
     5221 tgaccattgc cgattttgtt gccgatctgc gtgcaccaac accgtcggct gccgccgaag
     5281 tggtgagccg taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg
     5341 agatggcgat ggattattat ctcgccaacc gcacacgtcg ctttacgcaa attcatcacc
     5401 gattacagca acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgaacgcc
     5461 tgcaaaaacg gatgagcttt gcgctggaaa atcaacttaa gcgtgccggg caacagcagc
     5521 agcgattaac acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa
     5581 cgcgcattca gcaactggaa tatcgtttag cagaaaccct gcgcgcacag cttagcgcca
     5641 cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc
     5701 tggcgcgtgg atacagcgtt actactgcta ctgacggcaa ggtactgaaa aaagtgaagc
     5761 aggttaaagc gggtgaaatg ctaaccacac gtctggaaga cggctgggta gaaagtgaag
     5821 taaaaaacat ccagccggta aaaaaatcgc gtaaaaaagt gcattaagcc agcacgaatt
     5881 caacgcgttt tttcgatatc aagccatgcc cgtgctgaca gaagtaatct accgcaccgc
     5941 aggcttttaa cacctgcaac ggttgatggc agtccgggca aagagctttc atttcaataa
     6001 ctttcccgca ggatgggcaa tacgcatggc cgttatcctg atcaagcaca tgctgacatt
     6061 gtggacaatg cagttccata tgaattcctc cggtagccac ccctctacat tcatagaggg
     6121 aatggcagat aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg
     6181 cttacgttta cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc
     6241 cccttctttg aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta
     6301 gttcatcaag taacgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac
     6361 cacaatcggc ggattataac caccggcgtg tgcatatttc agcttcacac gacgaccgcg
     6421 taccagcggc ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt
     6481 ccccacacga cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac
     6541 accactgccg tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg
     6601 gaagtccagc gtttctttca cctgctcttt cacttcctga ctcaggccat cccacttatt
     6661 caccacaatg acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg
     6721 atcggaaata ccttcgcgcg catcaatcac taacatcacc acgttggcgt cttcaatggc
     6781 ctgcaacgtt ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg
     6841 tacgccagcg gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat
     6901 actgtcacgc gtcgtaccag gcatgtcata aacaacaacg cgctcttcac caagaatacg
     6961 gttagtgagt gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag
     7021 actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg
     7081 cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg
     7141 cagtagcaca tgctccagca gacttaatac gccacgaccg tgagacgcgg cgatcgggta
     7201 gatttcacct aaaccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc
     7261 agttttgttt gccaccagga aggtcggttt ttcacgggag cgcagatgtt tggcaatcgc
     7321 ttcatctgcc ggcatcaggc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc
     7381 ttcaatcgcc agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc
     7441 aatcccgccg gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt
     7501 acggtcacga gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg
     7561 gttaaataac gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat
     7621 gtttaaagcc tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta
     7681 aaaacaggaa aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta
     7741 acgtgtaata gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc
     7801 aaccggttca gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc
     7861 ttcgacgtta atccagtgca aataaccttc actgtcaccg accaccaggt tgccattata
     7921 cagcaccgga gaagtcagca ggcgatgcag cagatcgctt tgtgtccaca gcgtaacgcc
     7981 gccatcaatg gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc
     8041 gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg
     8101 cagatcaagc gccgtcaggt taccattata ggccagcgcg aaaacaacgc cgttaacaac
     8161 ggcgggagtc gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcttggga
     8221 aatacgctgc tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt
     8281 atcgcccccc acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga
     8341 gagcgaaggc atatcgaggt taactgtcca tttgacagcg ccgtcagctt cgttcagcgc
     8401 ttgtaactga ccgttactgg tgtggattaa caccagaccg tcgctgacca ccgggcgcga
     8461 aagtgcttca cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc
     8521 gtaaacctgc gccttttcgc tgccaatgta gacatgtcca ccagacacgg tcacaccgcc
     8581 agaaagtaat gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca
     8641 gatttctttg ccatcatccg cattcagcgc ttttactaaa ccagcgcggt ccgctgcata
     8701 gacaacgttg tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc
     8761 aacggaagtg ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga
     8821 catctttacc acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga
     8881 aagcagtcct ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat
     8941 tattaatttt catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg
     9001 cttcccatgc actacgcgca ccttgcttat cacctttgct cagcaatgct tcaccacgca
     9061 ggtcggcaac aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg
     9121 catcagcctg cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga
     9181 gattttcatc gctcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt
     9241 catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt
     9301 tattttcagc agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg
     9361 cggtaaccgc attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt
     9421 tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt
     9481 tgccattttc agcaaaaaag cgtttaatcg cttctacctg gtcgttttcg ttctcgtaaa
     9541 tttccacgct gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct
     9601 atcctgcgca actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt
     9661 agccacttca gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc
     9721 aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc
     9781 atcacgcaga cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac
     9841 caggtatata tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa
     9901 tacaagacgt tcgaggccca tcgcaaaacc gacagccggt gttgcacggc cgcccagttg
     9961 ttccacaaga ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgagactgtt
    10021 agtcacccac tcgaaaacgg tacggttgta gtaatccaga ccacgcacca gacgctggtt
    10081 tacggtgtaa gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg
    10141 agattcctcg tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc
    10201 cggatttttt gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc
    10261 gtccagcttt tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg
    10321 tgcttccagc gaaccgatag aattcagctc aagagttacg tgttcggaaa tacccagcgc
    10381 gcgccaccag cgggcggtga gcataatcag ttcagcgtcg atatccggac cttgcagacc
    10441 gaaaacttcg cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg
    10501 acggaacatc ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc
    10561 gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt
    10621 gcgatcctca aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg
    10681 tttgaatagc ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc
    10741 gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta
    10801 atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat
    10861 aaaaatgaac cctcaacgct tccctcaatg tttcgggagc catgcgggtt caatcataca
    10921 cgggaagcga ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga
    10981 cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg cctccagctg gtcgatcata
    11041 tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agagaccgct tttcttgttg
    11101 ccgccggtga cgccgagtgt agaaaccagc gcctcacccg ggccattcac cacgcagccg
    11161 ataatcgaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaact
    11221 gtaccgataa catcaaattc ctgacgcgaa caggtcggac aggcgatgaa gttgatacct
    11281 cgcgaacgga tacgaagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga
    11341 tcggccgcca gtgatacgcg cagcgtgtcg ccgatgcctt cagacagcag cagacctaaa
    11401 ccaatggcgg attttactgc cccgctgcgc gcgccacccg cttcggtgat ccccagatgc
    11461 agcggctgat cgatttgttt tgccagcaaa cgataagact caacagcgag gaagacgtca
    11521 gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatgg
    11581 cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct
    11641 ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag
    11701 tcaaccacca tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa
    11761 tcgacgccgt attccgctac tttcagcgca atgcgatagt cgaagtggat gtcagccacc
    11821 agcggcacgt taacctgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt
    11881 acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt
    11941 gcttcgacat ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg
    12001 ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg
    12061 ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat
    12121 tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc
    12181 cttgatactg gatctgtact gcggctggcg caccaatttt cagtttgtac ggtgcctggc
    12241 cggttaagtt caaattaccg tctttacgct gcataccgct aaacaatttt ttaccggtag
    12301 catcagtgac ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag
    12361 ccaccggcgt ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca tctggcgttg
    12421 ttgctgccgt tggtgccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgaaacaa
    12481 ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacgacaggt gtttgcgtgt
    12541 tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg
    12601 tcgacgtatt taacggaaca ctctgcccct gctcgctatt actgctcagt tccgccgaag
    12661 attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg
    12721 caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt
    12781 cgcgtttttt gcggcgttta ccgagggaaa aactctgcat cggcgcaact tttgcagccc
    12841 gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac
    12901 gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat
    12961 cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct
    13021 gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat
    13081 tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga
    13141 tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca
    13201 tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcactaag gctaacttac
    13261 tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga
    13321 caattgcaac ttaatacttc attcataaaa cattacgcca cggtacataa agtaaccgtg
    13381 gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag
    13441 ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc
    13501 atcaccacgc gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa
    13561 acggtcgata cggctgttcg agctgcgtcc atacggcgca gccgggaacg ggttccacgg
    13621 gatcaggttg atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc
    13681 agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc cctgattggc
    13741 gttggatttc tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt
    13801 gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag
    13861 cgcaacatcg atcatatcgc ccagtttatc cagcgctgga actacaccgg aagtggaaag
    13921 cgtcacgcga cgtttagaca gaccaaaacc gaaatcatca agcatgattt ccatcgccgg
    13981 aacgacgttg ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat
    14041 tgggcgctga ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc acacctggcc
    14101 aataatttcc gatacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt
    14161 acactccagc gcacacccca cctgcgaaga gacgcagagc gtagcgcggt cgtcttccgg
    14221 gatgtacacc gtttcgacac gctggtcacc cacggcaatc gcccatttaa tggtgccgtc
    14281 agatgaacgc tgttcttcaa ccacttccgg tgcgcggatc tccgccacct ctttcagttt
    14341 gccgcgcaac actttgttga tgtcggtcat ctcatcaaaa ttgtcgcagc aatagtgata
    14401 catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc
    14461 ccgcatctgc tgacgattga gatccagcag gttgattttt ccatctttgg tggtgacgtt
    14521 ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg
    14581 cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc
    14641 attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa
    14701 cgggtgcgcg ggcacacttc gccttcgccg aagaaataag cgatttcgcg agcggcagat
    14761 tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc
    14821 agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga
    14881 acggcgtttt caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc
    14941 agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgtgc ctgttcaacg
    15001 gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag
    15061 atattaccaa tgacgttttt tgctaccgcg ttcggtttga taatggaaaa agtacgttca
    15121 atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc
    15181 gcggattata atgagcaaca gagccgttga ctattgatga aggtaacatt tttttaaaat
    15241 aaaatgagtt ttagcaacaa ctcgactttt atggtgttct catcccataa cgatgacgtt
    15301 ggcaataaag acaattttgc gcattttgct gaaaagtaag aaaggatttt tgacaggttt
    15361 tacagacttt gcgattagca tgaagattca caatttttgc cggtagaatc gtcaattcaa
    15421 cggtcattgc ctgatgaaaa caaacagcaa tacaattttg gcatccgtta cacaaggcat
    15481 catttatttg taaatggcct tcgttaagcg aaaagacctg ttgctcgcag gcccgccagc
    15541 atgcaccgca aagctgacac ttttgaggat ccatcatcgg gcgagcatca ctaatctggg
    15601 gccataaata atgtaaccga cgtaatcctg gttgtatttg tgtcatctga acatccgcac
    15661 gctgcacatg gaacaaacga cgccgggaaa cattcacctc actggtatgt ggagatacca
    15721 cagaccagcc ttcctcgcca tattgccgta agcattcgtt taacagcaat aatgcgcgct
    15781 cccatgcctt cgcctgactc agatcaatcg cgatcgctgt gatcccctgc tctttatgcc
    15841 atagcaaaag ttcttccagc gtcggcgcaa tagtggtgaa aggtgcgacc agggtgtgat
    15901 tgcaaaaata acgtaaaggg gaattaacgc cacgaatagc ctcttgcgga caaataaact
    15961 ggcaaaccgc acaatcaata cattgttccg cgtcgaccgc tatgccctct tctgtcagcg
    16021 ttagcgcctg tgccgggcaa gcctcgacac aggcatgaca tgttgcaaaa cgaaagcgtt
    16081 ggcgcgcgca ttccgcgcca atcgttatgt tatgacttaa cattagccga tactaaaaaa
    16141 gacaaaacgc agcataactt cactccccac tagcattacg ctaccgaata acgccgccgg
    16201 gatattcttt ttacaaccac cgacagcaaa gaacatcatc ccaatgacgg aaattcccca
    16261 gcttagtgtc agtatgggtt ttaactgtcc cagcatcgct aatggcgcat gtggcaatgt
    16321 ggctatctgc atcgcatttg ccgttaaatc ccctatccac aacggctgaa ccaacaaacg
    16381 cagcagaacc aggagagcga tcaccaacac gcatccatta cgcagggcat caatctgcgg
    16441 caaaattccg ctaatacgaa acactgctac cagcgctgaa ccgagaattc ccactgtacc
    16501 taagaacata atcagcgtgt tgtaatgctg ccaggtgata atcgacgtat ggatgtaaac
    16561 ttgcgccatg cagaaaacat caacaatccc gaccactgcc gctagcataa gcaggagttg
    16621 ccagccgcct ttgcgtaaca tgaccagtaa tgtggataaa cccaatagcg ccagatacag
    16681 cgcaccaaag ataatttccc gactcagcca tgaactggaa aagtggctca gcgcatgcat
    16741 cgcattaagt ggataaccca ggtgtaaggt agaaatcagt aaccctacgg cacctgccaa
    16801 aaaagccaac agtaatggcg gccgcataac cgggcgggcg ctggaggtga ttaaaatatg
    16861 acgatgaaga aacgccagcc acaacgtcat accgaccgat gcctgtaaca acagcgtaaa
    16921 agccattaac ggtaattcat gcatgatgtg acgcctcctt ttccgcgccc tgatgcggtt
    16981 taatcaccag atttggctgc gtaatcgacg aatcaggtaa acctttcaca tcactgacat
    17041 cgccatactt ttcacgcaac tcatcaatag ggccgaactt aatcgccccc aacgggcacg
    17101 ttgccacgca aatgggttgt tctcctttag cttgcagatc aatacagaaa tcgcatttcg
    17161 acatctgccc ggtctgctca ttcatttgtg gtgcgccgta aggacaggac caggcgcagt
    17221 aaccgcaacc gacgcactta tcagtattca cgcgcacaat accgtctccg ggacgcttat
    17281 gcatcgccat cgtcgggcaa tttttggtac agatcggatc cgcgcaatgg ttacaggaaa
    17341 cagaaagggt ataagcaaag acattatgac tcacaccgcc ctctccagtg gcgatgaagc
    17401 cacctccgtt cacttcatac acgcggcgaa aacgtcgccc aacctcaagg ttgtttttgt
    17461 ctttacaggc cacctgacac gctttacagc ccgaacaacg cgaagagtca ataaagaacc
    17521 ctaattgttt gtcgctgaca gatgcaaatt ctttaaattg gctcatgctt tctcaacctc
    17581 caccagcatt gtttgatgcg aattcccttt cgccagcgcc gtaatgcggg tcgaactcag
    17641 tacattggca cagcctccgc gatcgatacc ttgtgcatcc ggttgccacc atgcccccgc
    17701 ctgcatagca actacgccag ggatgatacg ggggattatt tccaccggga taatgctcac
    17761 accacgatcg ttataaattt ttacgctatc tccttcgcta atcccgcgct gttttgcatc
    17821 ttgcggattc agccagagtt tttgcgtctg aacttcttgc agccacgggt tagcatactg
    17881 ggtggaatta gcacggtttt tccctttcca ggtgatgagc tgcaacgggt atttcgccgt
    17941 cagtttatct tctggccctt caaaggcagg gacatagtgc gaaagcgcgg gaatttctgg
    18001 atcttgcata tcaaaaagac gcttagagaa gatctcaatt ttgccggaag gcgtcgggaa
    18061 aggattattc tgcggatcgc gaatatttgc ttcgaaagcg atgtgcggcg cgcttttgaa
    18121 aagatgtcga cgtgtttttt gcagggttgc gaagtccggc aaattttcat ccggcatcgc
    18181 cagacgagtt tgctcccaga tatgctcgat ccattgcttc tcatcacgtc cttgactgaa
    18241 ctcggcttcg acacccagtt ttttcgccac gtcacgcaac cagtcataat cggtgcgacg
    18301 ttcaaaatcc ggctcaatcg gtttttctga cagaataaga tagctcgcag ttccccatgt
    18361 ttcgccgata ttccagcgtt ccataaagct ggtttccggc agcaacacat cggcatattt
    18421 cgcgctcgga gtcatgaaca gatcgctgag tacgatgaat tctattttag attcgtcttc
    18481 aaggagcttc gcggcctggt ggacgtccgg gttctggttc gccagataat tacctgccag
    18541 tgagaacagc aggcggatat tactgtcgag tttatcgaca cctttaagcc catcttgcgg
    18601 cgtaacttta gaagcatcat ccgccgcctg catccagttc ataatcgaga ttttcgcttg
    18661 taccggattt tctggcatat ctggtccaac gcagaattta cgattagaac taccgccgta
    18721 acctgccgcc cagccacctt tgatgccgac gttaccggta atcgaggcca gtaaagttga
    18781 acctcgggcg gtgcgttcgc cgcaaatatg tcgctgtggc ccccatccct gaatcaacgc
    18841 tgcgggtttt gtagtggcgt aatcgcgtgc cagttgacga atggattgtg ctggaacgtg
    18901 ggtaattttt tctgcccatt ccggcgtttt gtgtatccca tcttttgcgc caaaaaggta
    18961 ggcaaccaac gattcgttgg ctggtacgcc ttccggcatg gagttttcat caaaaccgag
    19021 agtatatgtg tcgataaatg tcttatcgtg gagattctcg ctgataatga cgtacatcat
    19081 cgcgtccatc aacgcattgt ccgtggtcgg caacagcggg atccactgat cggcaagtga
    19141 agatacggtg tcggaatagc gcggatccac cacgataaaa cgtgtaccgt tttgcttcat
    19201 tttttgaaaa tagtaattgg tgtgaccgaa tatggtttcc gttgggttat gcccccagag
    19261 aatcaccagt ttggtatcca gaagagtatc catcgagtta ccgctcgcgg ccacgccata
    19321 cgtgtaaggc gtggccgccg cagtattacc cagactaact gagtgataat attcaagata
    19381 accaccggtt aaatttaaca gccgacgcgc catcgcatcg ccagaaaatg ttccaccaga
    19441 aacagccgtc ccgacatgga cataacgtga agctgggcca tattgttgcg tgatcctttt
    19501 caggttatcg gcaattaagg tcgtcgcctc atcccatgag atacgctcaa atttaccctc
    19561 gcctcgttta cctacccgct tcatcggata tttcaaacgg tctggatgat aaacaaattt
    19621 acgatagccg cgaccacgaa cacaggcgcg cataatcggc atttgtggat ccaattcgtt
    19681 atcgggaagc gtggtaattt gcgtcaccac gccgtcgcgc atatgcgccc gaatatcgca
    19741 cttaccaccg cagtcaaatg tgctacaggt gggcacgact ttatcagcta ctggagctgt
    19801 aacttctggc gacgcatctg gggattgcgc ctttgcggta gaagaaataa aaggcaaagt
    19861 gataagcgcg gaagtcgctt gtaaaaaact acgtcgtgat acttgcggaa atgtaatgcg
    19921 ttccttttcg tctggcgtat ccatcttgcg ttcccgaaaa tgaaaaaata aatattcacg
    19981 tatttattcg agaacaatgc tgataacaat caaataatta gtcgtttaaa aaaacgtgag
    20041 attattaaat tattaatgca ttccaaatat aaataccata attagattaa taaatatttt
    20101 gtatatttca ctgaaatgaa aaatgtactg acgcgatttg ccctacgtca tccatcacca
    20161 gcaattgata atcaccttta tccgtcaaat gcagggtcac gttgcgcccg cgttcagtta
    20221 acggttcgcc attcagaaac caccagcgtt cacctgcccc tccacttgat tgcaacggca
    20281 aagtcgcttc cgctgcgccc ggtaaacgtt taataatctc gccatcgcga acgcctgtca
    20341 gctgcagcgg gagctgggca tcgtgaccgt atggcgggca aattgtcgag gccggtggta
    20401 agcgcacagc gcgacgttct gatgcgggca accagggttc cagcggtagc ggccagacat
    20461 ttatcatttc ttgtcgcgct tgcgggcaat cagcggcaac acgtttgcca ttttcatcca
    20521 gccagatggg gaaacgaatg ccattaatgc cttcctgctc cggcagtaat agagttggcg
    20581 gctgactccc gtccagcagc caggttgcca ggcggcggcg acagttaccg tcaccttccg
    20641 gcaaagactg tccgcccggc cagcaaataa cgccacgagt gactgagttc gggcgcgggt
    20701 cttccggcag attagcactg cgcgacagta agatattatt gacctgattc aacaatggta
    20761 cggcgctggc aaaaccaaac tgaccaacaa cgggcgtgcc gtccggtctg ccagtccaga
    20821 tcccaatgac atagcgagcg ttaaccccaa tcgcccaggc gtcacgatag ccatagctgg
    20881 tgcccgtttt ccatgccagt ggggcgacgc gcggcaaggc accatccggc aagggtagcg
    20941 cttcatcagc cataatccgg cgaatgatcc acgccgctcc cgacgacatt aaaggccgtt
    21001 caagcagcgg atcgtcaggc tgtaagcgca atttgcctgc cttgccgtgg cgagcaaacg
    21061 cggtatacgc tgccgccata tcttccagtt tcgcaccagc gccgccgaga atgagcgaaa
    21121 gattcggcgc agcaccgttg ggcaaatata acggcaatcc aacattgcgt aactttgccg
    21181 caaaccgttt cggtccatag gcttccagca cctgcacagc aggtaaattc agcgagcgca
    21241 ccagcgcctc gctcatgctg atcgggccat gaaaaccgct gtcaaagttg ccaggtcggt
    21301 aatcaccggt acgccggggg acgtcttgca gcagtgatgc cgggtggatc aatccttcat
    21361 ccagcgccag accgtaaaca aacggtttga gcaccgatcc tggcgatcgg atcgcattga
    21421 ccatatcaac atgaccaaag cgtgaatcat cgttgagatc aaccgaaccc acccagccgc
    21481 gaacacgcat atcggtatga tcaaccacga tcatcgccag tgagctgcgc ggtggtaatc
    21541 gtcctttcca gttttgcgcc agttcttcca gacgtcgttg aagaccggca tccagcgtag
    21601 taacgatttt gtcgcttttg cttttaccga gcatcatgcg cgaaaacagc ggtgccagtt
    21661 gcggcatttg tcggggtgcc agccagatgg gttcttccct tgactctttt acctgctcac
    21721 gggaccacac accttgcacg gccatccgtt cgagcacttt attacgcgcg acttcggcac
    21781 gctccggcca acgatccggg cgaagacggc tgggcgcttg cggcaaaacc gccagcattg
    21841 ccgcctcgga atagcttaaa ttcgcaggcg actttccgag ataagcccaa cttgccgcac
    21901 cgatcccctg caacgtaccg ccaaacggag cgcggttaag atacaaggtc agaatttcac
    21961 gcttagacag atgccattcc agttgcaacg cgcgccagag ctggcgaatt ttgccgccaa
    22021 atgttttggg gtgaggatca agcagacgag caacctgcat agtgagcgtg ctgccaccgg
    22081 aaataacccg tcccgaagtg agatcttgcc atgctgcgcg cgccaccgag aatggattca
    22141 ccccaggatg cttccagaac cagcgatctt cataattgat cagcgcttca aggtaacgtg
    22201 gagaaacatc ttcgattgtt acaggataac gccagatgcc gtcagcatcg gcgaagcgcc
    22261 agagcggcgt accatcctgc gccacgacca ctcgtgcggg attgacttca tgcagcggta
    22321 gaggccagag tttatctgct ccccacgctg caagaaaaag gagaaatggc gcagctgcca
    22381 gcgttatcca gcagccgcgt ttggttaaca agcgaggcat ttacggtctg acaatcagca
    22441 aatcttcagc cgcgccggtc gcccgccatt ggggaacata cattgattcc accatcggtt
    22501 gcggtacctg atacgttccc ggcgtcaccg cccgcgccag atacaccagc gttaccggtt
    22561 ggtattcatc aacggcaacc gccgccacaa agcgatcgtc acggaactca atgtgcttaa
    22621 tactcgcctg ctgcatctgg ttcagtaagt tctgcacttc gccaccactt tgctccaggc
    22681 tggcgctacc gttcgccaga ttctggtttt ccagttccag acccgcaggc agcagatcca
    22741 cgactaacgc atccggcacg ctgttactag cttttacctg caaccagacc agcaccagat
    22801 cgccgctacg taacgagtcc agcgatttgc tcttaccatc agtaccaaga atatgacgct
    22861 caatttgcag cacattgttc gccggtaaag gtgcggattg cggataaccg ctggcatcca
    22921 cacgcagcca taacggctgc tcgccactgt tggtcacctg caaggtggca agttgatcgc
    22981 tattcagatt gctgttttgc gctttctcgc ctgtcagcgg ctcagctgag aaagaggttt
    23041 gcgcctgcca tttacctggt aaatcctgaa tcgtacgggc agccaggaac aacgcgttac
    23101 tttcctgcgt cgatagccag cgttcaccaa acgcctgctg cgaaagtgtg ttcagcaggg
    23161 agtactgctc atcgggtagc agtttgttct cttccagcaa ggagagcatc aacgcgttgt
    23221 cgcgcagtgg actaccgtaa tcacccagcc atatccgctc atcactatta cgcggcgttt
    23281 ttagcgccag cgcaatcgct tcttcatcac gcgtcgcatc acccatggtt ttcagcgcaa
    23341 cgccaagttg cagcagcggt aaaccagaag cggcatctgc acgatgctcc cagatttcac
    23401 gcagcgcacc cagcggagcc ttttgctgac gggccaacac cagcgcagcg taagactgta
    23461 cggcgaattt actggctttg agattatccg cgtacgggat cgacatcatg cccggatctt
    23521 gtaaatagcg cagcagacgc tcattacccc ggttaatggc gtctgtcggc acgctgtaac
    23581 cctgttcgcc tgcgcggacc aggaaatcca tcacgtaagc cgtcagccag tactcttcgt
    23641 caccgttttt atcccacagc gcaaagccgc cgttatcacg ttgcatttgc agcaaacggg
    23701 aaatgccgat atcgaccgat gcacggcgtt tctcatcact gtcgcctttg atgcccaacg
    23761 cctgcagttg ggctgcgttg gtataaagtg acgggaacag gccgctggcg gtttgctcaa
    23821 gacagccgta cggatacgct tttaactctt tgatataacg tgcgatgttc agcggtggtt
    23881 tgccgctcaa caacaattgc ccttccagcg taacaggcga gaagttttgc aatccatccg
    23941 ccggaatcgc ccatgtctca ccaggctgta acgccgtacc gtaattaacc gtttgtgccg
    24001 ggaacgccgg acggacgccg attttccact gcttatgctg atcggcaacg gtttcacccg
    24061 gtaacgctaa cccgctaatg gtggcctgaa tttctccatc gccataaccc ggcaatgctc
    24121 gcaccgggat aaacagcgta gtacgcacac ctggcgctaa ttcaacggct gcgggtgaat
    24181 cgctgaccag ttcaagcaaa ccactggcgg tcagggcaac gttcagtttt tgcggtttat
    24241 cggtaagatt agtgatatcc agcgtcagac gcgagttatc gccactcgcc ataaagcgcg
    24301 gcatgttcag ttcagcaatc accggtgcgg caactatcac tttgctttcg ttgctaccga
    24361 attcatctgc cgtccaggct tgcgccatga cgcgcaattc accgttaaaa tcgccaatcg
    24421 gcagtgtaac cgagccttcg ccctgttcgt tgagcgttac cggcagcgcc tgctgcgcga
    24481 caatattgac gtgattgacc ggcggtttac caccacgttt cagctcatca ccatcgccac
    24541 cgaaacgcag agctgccaga cgcccctgac cttcaataac ctgaccgtaa atatcgtaaa
    24601 tgtctgcgcc atagcgtttc tgaccaaaga acgcctgcca cggatctggc gtgacgtagt
    24661 cagtaatatt cagcacacca ctatcaacgg cagacaccag cacattcacc tgtttaggct
    24721 tctcgccatt tttagtgctg gctttaattt tcacggttaa tggttgattg ggacgcattt
    24781 ttgctggtgt ttccagcgcc agatcgaggc gacggttttc atcaccaagc ggcagatgca
    24841 acacaccaac cgcgcgtttt ggcgtcgcgg agcgagattt atcgccagga cgcaccacca
    24901 gcgtactcaa ataaagatca tgacgattcc aggttttatc gaccggaatc gtcagatcca
    24961 gcccttgagc cggaacatca atctcttgcc accacagcgg cccttcactg gactcgacca
    25021 tcgcataacc tttacccgcc gttggcgcgg cgatatgcaa cttaatggta tcgccagggc
    25081 gataaccggc tttatccagt ttcagcgtga cacggtcggg tcgcactgcg ccgctaccgt
    25141 cgctattatc ctgccagcta tagccagccc aaaaacgaac actactgacc gcttcattcg
    25201 gcgctttgac ttccagacga taagcgcccc actctaccgg gaaactgacc ttgccggttt
    25261 cgtccgcttt cagatccaga gtttgttcat tttcaatcag atctttttga tcaaactgtg
    25321 attgccagcc ttcatcttct gaccagttcc agtagtaatc gcggcgttcg cgaatcaggc
    25381 gcacctgcaa gcccgacacg gcttttttca cgccttgcgc atcgctataa acgatgtcaa
    25441 aagcggcgtt actgccttca tcaacaatgg gctgtttcac cgtgctgtca gtgcgataat
    25501 cgtaaaccga tttcgaggcg aactgcggac ggatccccgg caatgcatcg gcaggccaga
    25561 tagcctgctc agcgcggcgc gtcaccgggc gaccgcccga ttccagcaaa ctgccctgga
    25621 aaataacctg caatggggaa tgcgtttcct tccactggct ttctgtagaa acttcgccgc
    25681 gccctttatc atccagcgtc aactgaactt catccagcgt gcgggaaagg ttttcggcag
    25741 ctatatcgcc gaattcaaaa ccaggtaagg ctgacacagc ctcacgcagt gggcgcagga
    25801 aaagttgccc ttgcaaagta ttaccattag caggcgcacc gtacaggtag tatcccacca
    25861 cggagaattt cacttcatca ttcggcgtaa gcggggtttt ctcaccggtc agattcagcg
    25921 ccatacgctc tggcataaaa tcttcgacgt ggaaatccca catccgatac tggttatcgc
    25981 ccgtgttagc gcgaatatgc cacataccgg ttgccgcatt gctatcgagt ggccaggtaa
    26041 agtggtagag gccattttcc ggctgactaa cgacgctcct gagtacctgc ccatcgggtt
    26101 taatcacgtc taacttgatg ggttgattgg gcaacgcttt accgtctgca tcacgcagca
    26161 aaccattgag gattaccgtt tcacccgggc gataaagatc gcgcgggcca aacatgaaaa
    26221 actgtttgct atagcctggc gcgccagcaa tgttaaattc tgctaagtcc agcgccggaa
    26281 gttttaaatc gagtagcgtt gtctgaccgt ctttacgtgc cagcaataat gcggcgtttt
    26341 tatcattttc cagctgcaca tgcccctgag cgtcactggt tgcctgagtc agagtctgcc
    26401 ctttctcatt taataaagag acttcaattc cttgctgggc cgcgccgttt tccagacttt
    26461 gggtaaagat atccagacga ttgtgataac ggtgagctga aacgccgata tcacttaacg
    26521 taaacagcgt cgcgggatta ctgtaatcgt aacgtccagc ctgattcatc acagccagat
    26581 acacgcccgc ctgctgaagc ggtttgatat cgcccagcgg cagcaataat ttttcacggg
    26641 tgttacgcgc aggattgaga tcaaaccgtc cggtgtagac cagatccgcc atctgcagca
    26701 gtttgtctga ctgccagttc gccagcgaat tgcggtattc ccattggcta atgaatgctg
    26761 gcagagattc tggcttaacg cggaagaagt taacatcgac attattaacg ttgagcgcca
    26821 ttaccggcag cccttcaacg actttaccag gcagcagcga accacggctg gcaaaaccga
    26881 cactgggttg gatgtcgcgg gtagttatag ttttttcgta atctttactg aaggttgcgt
    26941 tgttgagtgc tttgacctcc ttgccaattg taacgatcaa atcacgtttc ggttcgaggt
    27001 ggcgtaaacg cagctcttta agattatctg atagctccca ggcaccatcc actttgccgc
    27061 tttttttatc gacgacatga ataacgcgtg agaaatcctg atccgggtcg agagggatgg
    27121 agaacgtcag caccagcgtg gctgcaccat caagttggac ttcagagaga tccagcaatg
    27181 tcagcgcctt accggcactc tgttgggcca gtttttgtcg ctctggcgcg gagagttttg
    27241 ctgaactcgc gttttctgaa gaggccgctt tagtaacttc agaaggcgca tcttttttca
    27301 ccgctgttgg cgcgttatcg ttgttgtcgc accctgccag cgccagcatt agcatgcagg
    27361 cggctacgcg taactttttc atttttcatc cctgccacaa tggcccgtta gcaacgtcga
    27421 ataattatta tgcgtgagaa tttacgttat gtaattcagt atcacgccca tacaatccga
    27481 attttagtga gaattgtggc tcagacagcc aaatcagcct cttttgcttg tcgcttttca
    27541 ctaaacatcc gacaatttag cctaaccccg gcaaaaatgg agatgcctat gtccacgaca
    27601 tggtttgtag gagccgactg gctcgccgaa catattgatg acccggaaat tcagattatc
    27661 gatgcccgca tggcgtcgcc tggacaggaa gatcgtaacg ttgctcagga gtatctgaat
    27721 ggacatattc ccggcgcagt gttttttgat atcgaagcgc tttctgatca cacttccccg
    27781 cttccgcaca tgctgccgcg cccggaaacg ttcgccgtgg cgatgcgtga attaggcgtt
    27841 aaccaggata agcacctgat tgtctatgac gaaggtaatc ttttctcagc cccacgagca
    27901 tggtggatgc tgcgcacctt tggtgtagag aaagtgtcga ttctgggggg tggacttgca
    27961 ggctggcagc gcgatgatct gttgttagaa gaaggtgcag tagagctgcc ggaaggagag
    28021 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
    28081 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
    28141 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
    28201 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
    28261 ggtcgcggcg tcagctatga caaaccaatt atcgtcagct gcggctctgg tgtaacagca
    28321 gccgtggttt tgttggcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
    28381 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccattgaa ataagctttt
    28441 tacaggcaat aaaaaaccgc cgaatttggc ggttttttat tgctggtctg gttcgcggcc
    28501 tttccagcag gttgacttgt gttgccggag caacgcaggc accccacagt gttgaaatac
    28561 tctcttttat gcctggctgt gtcaagataa taaacagagt aattattcaa acatgagagt
    28621 attattctta atggcccttt tcgccgtctc gcaaacgggc gctggcttta ggaaagaatg
    28681 ttccatgacc gtaaatacag gcaccccaca gtgcttgcta tcgcggcaat atcgccagtg
    28741 gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga agatacggtg cttctgtatc
    28801 gtacttattg tttctggtgc gctgttaacc gaggtaaata ataaccggag tctctccggc
    28861 gacaatttac tggtgattaa caaccttcag agcagcaggt aagcccgaat gccgcccttt
    28921 ggggcggcat attttagatt atccgattct gtttaaagtc acgcaaaaaa ccaccccagc
    28981 gacgttcata gaatggcgca atatgttcgg taataaagtg gctaattcct ttttcccctt
    29041 ttttcacctg acaaatatcg attggttcat cgccaggtaa tgtatcggtc gctacacttc
    29101 ccgccacctg aataatttct tcgatatcac catcggcttc aatgccaata agtaaattag
    29161 gctgtgcctc ttcgttctct ttaattgaac aaataaaagc acgcttcaca ggcttaatgg
    29221 ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg cggctctgcg acttccgata
    29281 atatcagcga ttcaccgcct tccaggattt cctggctgct cagcggattt ccctcttcac
    29341 caatcaacaa actgatttca cgcggcataa attctttacc ggtcggcagt ttggcattaa
    29401 ggaagagcgt ttcgccaagg gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg
    29461 cctgttcgtc ttcaaccgcc tgttgaagtg cttctaacga ggtaaaaaaa ggaatgacgc
    29521 tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc gctatcttca accacagcct
    29581 cgccctgcgc cgccgtacca ggcacccaga cggtggattc cagtagagta cggaaaaagg
    29641 ccgggcggtg cgccggttcg gttgctgctt tttccagcag gtcttcaagt tcattttttg
    29701 tttcggacat aagaaccaca attcattcaa cgttcggggc acaagtgcct gatgcgccat
    29761 acttatcagg cctacaaggg attcacaatt tgttgaattt gcaatacttt gtaggccgga
    29821 taaggcgttt acgccgcatc cggcatatta gtttacgccg ttaacagatt agcaatcgtg
    29881 cgcacaccaa gtcccgtagc acccgcagac cactgttcaa ccggcgcttt acggtaagtc
    29941 gccgagcagt cgatatgcag ccagccttgc tgatagttct caacaaagtg cgacaggaag
    30001 cccgctgccg tgctcgcgcc tgccggatac gccgcgcttc cggtattgtt cagttcggca
    30061 aagttagacg gcagctggct gcggtggaat tccgccagcg gcagacgcca gaatggttcg
    30121 ttctcttgtg aggcactcgc cagcaaacga ccggcaagcg catcgtcaaa actgaacagc
    30181 gcgtgataat cattacccag cgcagttttc gccgccccgg tgagggtcgc cgcatcaatg
    30241 atcatttccg gtttctgcgc actggcatca atcagaccat cggcaagcac caggcgccct
    30301 tcggcatcag tgttcatcac ttcaactttt ttaccgttgc gataagtgat gatatcgccc
    30361 agcttgaacg cattgccgct aatcaggtta tccgcacagc agaggaacag cttcacgcgc
    30421 ttgttcagtc cgcgcgtaat ggcaaatgcc agcgccccgg taaccgttgc cgcgccgccc
    30481 atgtccgact tcatcgagtc cataaacgca gtctgtttga tgctgtagcc gccggagtca
    30541 aaagtgatac ctttacctac caggcacgcg tacactggcg cttctttatc gccagttggg
    30601 ttgtaatcca gcgccagcaa taccggagaa cgttctgaac cgcgtccgac ggtgtgcagc
    30661 cccatataac cttgctcacg cagatcttcg cctttggtga tccgataagt cacacgatcg
    30721 ctcgcgacgt tgctgatcag atcaacagca cgctgtgcca gttgcgatgg tcccaattct
    30781 tctgccggtg cgttgatggt gtcacgcacc cagtcgatga tcatcaggcg gttatccagt
    30841 tcctggcgct gggcatcgtc cagatccggc cactccactt tacgcgtgcc tttcggggct
    30901 ttgtaacctt gccagaatgc ccagcatcga tccgcatccc agccttcacc gcttaactga
    30961 acatgcttga tgcccagacc gtcaatcttg cgcgccgcac gctggatcag ccccagatcg
    31021 tctgccccgt tcaaatgcag ggtaatgccg tcattattaa tgctgtaagt tgctttttct
    31081 ccccagcgcg cgtcggcagg ttgggtagag agggtaatct tcatcgcttc tgtcatttta
    31141 gttatccttc tttgtaaaag ggccgccagt tggcagcccc aggatttttc cgctaaatga
    31201 ttcgcgttgc aggaaggcgg caagtgagtg aagccccagg agcatagata actatgtgac
    31261 tggggtgaac gagcgcagcc aacgcatctg cggcgtgaag catgacgcgg aaattattcg
    31321 gcctcgtcca gccagactaa caaaatcgct tcgaggattt tctcgttgga tgcctgcggg
    31381 tcgtcgtcga aatcttccag atcgcaaatc cactgatgca tatcggtgaa tcgaaccgtt
    31441 ttcggatcaa gatcgggata cgcatcgtac agtgcttcgc caatttcgcg gctatcggtc
    31501 cacttaagtc ccatactaac ctctgttaat gctcacgcgc atggttgatc gtgtaacgcg
    31561 ggatttcgac cactaaatct tcgtcggtga cgcgcgcctg gcagcttaaa cggctttccg
    31621 gctccagtcc ccaggctttg tccagcatgt cgtcttcctg ctctgagctt tctggaagcg
    31681 agtcaaaacc ttcacgaacg atgcagtggc aggtggtgca agcacaggat ttttcacagg
    31741 cgtgttcaat ctcgataccg ttacgcagag ctgcgtcgag aatggtttca ccgctattag
    31801 cttccagaac agcgccatca gggcagagat cctgatgagg caaaataaca atctttggca
    31861 tattaaacct cgtccacgga atggcctttc agcgcacgac gaaccgactg gtccatgcgg
    31921 cgagcggcga aatcctgggt ttgtttgtct acgtttttaa tcgcttgttc gatggcgtca
    31981 acgtcatcgc cctgcgccac ttcactcagg tgagcggcag catcgtcaat gacctgacgt
    32041 tctgcggcgc ttaacagcgc ggcatcagca gccagcgcgc cgtgcagact ttccagcaca
    32101 cgcgccgctt ctactttttg ttctgccagc attcgggctt ttacgtcctg ctcggcatag
    32161 ctcattgagt ctttgatcat cgaagcgatt tcgctatcgg tcagaccgta agacggtttg
    32221 acctgaatag acgcctcaac gccggtggat ttctccatcg ccgtcacgct caaaagaccg
    32281 tcggcatcga cctggaacgt cacgcgaata tgcgcaccgc cagccggtag cgccggaata
    32341 ccacgcagcg caaaacgcgc cagtgagcgg cagtcctgca ccagttcgcg ctcaccctgc
    32401 attacatgga tagacatcgc cgtctgacca tctttaaagg tggtgaaatc ctgagcgcgg
    32461 gccactggaa tagtggtatt acgcggaatc actttctcca ccaggccgcc catcgtttcg
    32521 aggcccagcg acagtgggat cacatcaagc aacagcattt cgctgtctgg cttgttaccc
    32581 accagaatat ccgcctgaat cgccgcgcca atagcgacga ctttatccgg gtcgatggaa
    32641 gtcagcggtg gacgaccgaa aaattcgcct acccgttcac gcaccagcgg cacgcgagta
    32701 gaaccgccca ccatcaccac ttccagcact tcatcagctt ctacacccgc gtctttcagc
    32761 gcgcgacgac aagccaataa ggttcgtttt accagtggcg cgatcagttc attgaattgt
    32821 tcacggctga tttcgccctg ccagcccgca acgttaacgg tcacggagtc cgcatcgctc
    32881 agcgcgattt tggctgcaat ggcggcatcc agcagttcac gctgaacgcg gttatcgcta
    32941 cgatcaggaa tgcccgcctg ctcgcgaatg taatccgcca gcagatggtc gaaatcatcg
    33001 ccgccgagcg cggaatcacc gccggttgcc agcacttcaa acacgccgcg acttaagcgc
    33061 agaatggaaa tatcaaacgt cccgccaccg aggtcataaa cggcgatcac gccttcctga
    33121 ccggaatcca gcccgtaggc gatagccgca gcggtcggtt cgttaagtaa gcgcaggacg
    33181 tgaaggcccg ccagacgcgc cgcgtctttg gtgccctgac gctgggcatc gtcaaagtac
    33241 gccggaacgg tgataactac accatccagc tcgcctgcca gggcttcagt tgcccgcgcc
    33301 gccagtgctt tgaggatgtc cgcagaaacg cgcaccgggt tcagcagccc cgccgccgtt
    33361 tcaatcatcg gcaggccgtt ttcgctggcc tggaattgat aaggcagatg cggatagcgt
    33421 tgctggatat cagccagcga gcgtcccatc aggcgtttaa cagaactaat tgtgttggcg
    33481 gtatcgagcg ctgcattagt acgcgcgtca taacccaccg aatgcccttg ctgttgatag
    33541 tgaacaacag atggcagcag gtgacggcct tcatggtcgg ctaacgtttc ggcctgaccg
    33601 ctgcgcactg tcgccaccag cgagttggtt gtgcccaggt caataccggc cgccagacga
    33661 cgctgatgcg gcgcagcact caaaccaggt tcactaattt gtaataaggc catgtttagc
    33721 ttccagaaat taaaaatcga gcagtttttc ttcgagttgt tcggcactgc ttcgcagttt
    33781 atcgagaaaa cgcagcttac gcacggtatc cgccgccgcg tcccaggtct cgttgtctaa
    33841 ctgttcaacc atcaactgat ggcgggtatc aaacatcttt ttcacacgtt tgataaagct
    33901 ttccagccgc gcttcatctt tcgcctgttc gatctcgtcc agctcttcgc gcagctccaa
    33961 ctgttccatc aggaacgcgg tgtcgcgcac agtatgctgc tcgctggcga gatcaaagcc
    34021 gtgcaaagaa agcaaatatt ccgcgcgcat taacggatga cgcagcgttt gccaggcctg
    34081 attaatggtt gcagattgct gtacggcggc gagttgttcc gcctggcttc cgctggcgaa
    34141 tttatcagga tgatactgac gttgtagatc ctgaaaacgc aggctcagcg cctgggtatc
    34201 gagttgataa cgggcaggca agccaaagag ggtgaagtaa tccataacat tctcagggct
    34261 gcgatccgcc gcgataacgg cgaatcaggt aaaacaaccc ccacgcgcag gcgaccacgg
    34321 tggggttatc ggtatgcgca tcaaacgtgg aagctttcgc cgcaaccaca ctcatcttta
    34381 acgttcgggt tggtgaattt aaacccttcg ttcaggcctt cttttacgaa gtccagctgc
    34441 gtaccgtcca gaaattgcag gcttttgcca tcgaccacga ctttcacgcc tttgtcttca
    34501 aacacgatgt cttccggcgt cggttcgtca acaaattcca gtacataagc catacctgaa
    34561 catccggagg ttctcacgcc cagacgtagg ccaaaccctt tgccacggtt agccagaaag
    34621 gtatttactc gcgctgctgc actgtcgcta agtgtaatcg acataaccaa acctcaactc
    34681 ttattttgct tcacgtttgc ttttatagtc cgcaatggcg gctttgatcg cgtcttctgc
    34741 cagaatagaa cagtgaattt tcaccggcgg cagttcaagt tcttcagcaa tatcggtgtt
    34801 tttgatagcc tgcgcttcgt cgagagactt ccctttcacc cattcggtga ccagggagct
    34861 ggaagcgata gcagaaccgc agccgtaagt tttaaaacgc gcgtcttcaa tgataccttc
    34921 atcgttgact ttaatctgca acttcattac gtcgccacag gccggtgcac ccaccatgcc
    34981 gctgccgacg ttctcgtcgt tgttgtcaaa ggaacccacg ttacgcggat tctcgtaatg
    35041 gtcgataact ttttcgctgt aagccattat aaattctcct gattccgata ccgattaatg
    35101 atgagcccat tcgatgctgt tcagatccac gccctgcttg tacatttccc acagcggaga
    35161 aaggtcacgc agacgaccga tggatttacg aactaactcg atggtgtagt cgatctcttc
    35221 ttcagtagta aaacgaccta aagagaaacg gatagagcta tgtgccagct cgtcgttcag
    35281 ccccagcgcg cgcagcacgt aggacggttc gaggcttgct gacgtacagg cggaaccgga
    35341 agaaaccgcg agatctttca gcgccataat cagcgactca ccttcaacgt agttgaagct
    35401 gacgttgaga atgttcggcg caccgtgttc caggtcaccg ttcaggtaaa cttcttcgat
    35461 atctttgatg ccgttccaca gacggttacg caggccgcgc agacgttcca tctcggtcgc
    35521 catctcttct tttgcgatgc gataggcttc gcccattccg acgatctggt gaacaggcag
    35581 agtgccggaa cgcataccgc gctcgtgacc gccgccgtgc atttgcgctt cgatgcgtac
    35641 gcgcggttta cgacgcacat acagcgcacc gatacctttc gggccataga ttttgtgacc
    35701 ggagaaagac atcaggtcaa ctttcaactg gctcaggtcg ataggcagtt tgcccacgct
    35761 ctgggttgca tcaacgtgat agataatgcc acgagcacgg cacatttcgc cgatagccgc
    35821 gatatcctgc accacgccga tttcgttatt tacgtgcatg atagaaacaa ggatggtgtc
    35881 gtcacgcatc gctgcttcga gttctttcag gtcgataata ccgttacgct gcggtgcgag
    35941 gtaggtgact tcgaaacctt cgcgctccag ctgacggcag gtatccaata ccgctttgtg
    36001 ttcggttttg ctggtgatta tgtgcttgcc ttttttctga taaaagttgg ctgcaccttt
    36061 gatcgccagg ttgtcagact cggttgcacc agaggtaaag acgatttcac gcggatcagc
    36121 gccgaccaga tcggcaatct gattacgggc gatatctacc gcttcttcag cctgccagcc
    36181 gaaacggtga gaacgggagg ccgggttacc aaaggttccg tccatcgtca taaactgcat
    36241 cattttctcg gcaacacgcg ggtccaccgg cgtggttgcg gagtagtcga gataaatcgg
    36301 taatttcatt gctctataaa ctccgtacat cactcaatgc aaggaaccag gctaccggct
    36361 ggatgtacga ccgtgtttac ggagcgcgat acgccccggc ctgattctga atacttttta
    36421 ttaagcgcgt aacttaacgt cgatcgcgtc ttgtgtgcgg gtgcgtggcg cgtcgtgagt
    36481 atgctgacga ccagacacat ccagcacttc ctggttgtta accagttcgc ctaaagtaat
    36541 gttattgaga aaaccggtaa gacggtcgct caaatcacgc cacagcgcgt gggtcaggca
    36601 tttatcgccg ccctggcagc cgcctttacc ctgacaacgg gtggcatcta cagattcgtc
    36661 aacggcgcta atcacttcgc caacggcgat gctgctggca tctttgccta acagataacc
    36721 accgcctggt ccacgtacgc tggaaaccag accattttta cgcagacgag aaaacagttg
    36781 ttccagataa gaaagggaaa ttccctgacg ttcggaaata tcagccaacg gtaccgggcc
    36841 cgcttcagag ttgagcgcaa cgtcaagcat tgcggtcacg gcatagcgcc ctttagatgt
    36901 cagtctcatg tcttacttca cctcaaactc gcccctgccc ggggtttttt attgtaaaag
    36961 tgggggtatt gcatagcagg gtcaagtctg acattcccga gtaaattggt caactattta
    37021 cttgactgat ttagtcgggt atttaacctt cagtgccatt tttttatcgt ggcgtttgta
    37081 cctgttatcc ttccagacca tgggcgggtt gagggcaatt attcgcatta gcgcaggcct
    37141 gttcccctca ccctaaccct ctccccgaag gggcgagggg accgtccgtg cacttatctg
    37201 gcttagccct caggccttat tacctttatt ctgctgctct atagaagcca gaatcccgcg
    37261 caggatattc aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt
    37321 catcacctgc cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag
    37381 atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac
    37441 ctgctcgcca ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg
    37501 aaccgccatc gccaggttca gcgagctgta ttcagggtta gcggcaatcg cgacatgata
    37561 atgacacttc tgcaactctt cattggtcag gccgacacgt tcgcgaccaa acaccagcgc
    37621 caccggggta tttgccgctt cagcaacgct tttcagaccg cattcgcgcg ggtcgagcat
    37681 cggccacggc agcgtgcggg aacgtgcgct ggtgcccacc accaggctac aaccggctaa
    37741 cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccccgccgc
    37801 cagggcaatc gcctgggagt caggtttcac cagtggatta accagccaca gattggttaa
    37861 tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac
    37921 cagcacaatt cgaatatttt gcagcattgt ctttcttcgt ctaaagatta ttcgcgcatc
    37981 ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt
    38041 ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc
    38101 gcgcaaggcg ggtaatttga ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag
    38161 ccagaaaggc agtaacgact tcgtgaccaa cgtagataaa gctgccgaag cggtgattat
    38221 cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact
    38281 tgaaggtact gatcaggatg ttcaatgggt tatcgatcca ctggatggca ctaccaactt
    38341 tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga
    38401 agttgctgtg gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg
    38461 cgcacagctg aacggctacc gtctgcgcgg cagcaccgct cgcgatctcg acggtactat
    38521 tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt
    38581 cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct
    38641 ggcttacgtc gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gcccgtggga
    38701 cttcgctgca ggcgagctgc ttgttcgtga agcgggcggc atcgtcagcg acttcaccgg
    38761 tggtcataac tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgttaaagc
    38821 catgctggcg aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg
    38881 ggtgatatca ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc
    38941 tttactttct ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc
    39001 gcaaggaaag gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct
    39061 ctttgttgtt gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta
    39121 tcctgacgat aaaatttacg gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc
    39181 taaggacggt actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa
    39241 cgccatcgca accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc
    39301 gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt atcgcgggtt
    39361 tggtaaatca aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat
    39421 caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag
    39481 tattggcggg gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc
    39541 agtgatcctc gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg
    39601 cagtggctac ttacttgatg agagttacag cggcgaaaat tatatcgcca gcgtcagccc
    39661 gatcccgctt ttactcattc acggtaaagc tgatcacgtt atcccatggc agcacagcga
    39721 aaagttgtat agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca
    39781 cattgatgct ttttccgatc gtcacggcga cgtttatcgc gaacagatgg tgaactttat
    39841 ccttagcgcg ttgaatccgc agaactaacc catgatctct agcacgataa tcattcacaa
    39901 aatcacctta agacatgcta atccactggt caggacagtt taagatgaga aaaattctgt
    39961 gacgcttgcc aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt
    40021 agctattctt atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac
    40081 catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg
    40141 ggttgaccgc caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca
    40201 gtcttgatat cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga
    40261 ctggttatga cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat
    40321 ttcactggct acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc
    40381 ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca
    40441 atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg
    40501 atattcattt cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta
    40561 cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag
    40621 cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtacca cctgatgagc
    40681 cactgtttat ggcactactt ttttccatgt tgcgggttcc cgatccattg cgggatgcgc
    40741 atcagcggga cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc
    40801 tgggaaatgt tcgtttttat gatgaacagg ggttatgtga tcagctttat acccacctcg
    40861 cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt
    40921 tcgccagact gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa
    40981 gtgaatacgg cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg
    41041 cctggctaat gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg
    41101 atagcgagcg agaagcgcag attgagcaac agttacgcga actaacgtta ctgccgctca
    41161 acattaaaca tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac
    41221 tgattattgc tccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg
    41281 acctgacact gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag
    41341 ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat cacccagaac
    41401 acgccgtggc ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg
    41461 ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga
    41521 gcagcaatat agcgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt
    41581 tgcaccacta tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg
    41641 ccgcaaatcg ctgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta
    41701 ctcagtgcaa agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc
    41761 gaggccgagt agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt
    41821 gccccctgca ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac
    41881 cacgctgacc aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga
    41941 cggatgagaa agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc
    42001 cgataatcaa acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc
    42061 gagccccaca gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc
    42121 agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc
    42181 atcagccacg ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcagcagt
    42241 gccagcacgc gcaaggcgga aatcaggcga gaaggatcgc tgacgcgggg cgcgatgagc
    42301 aaactcccga gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc
    42361 gttaaaccaa tccctttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa
    42421 aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc
    42481 cgtcaggagg cgttagtctg gagttaatct aatgttggtg caagttgaaa aataagaata
    42541 ttcgagcctg cttcccgatg tctgtaacgc cagttcacaa gcactcaaaa actgcgatcc
    42601 gtgacgcatg ttgcaaagat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc
    42661 acaatggcga tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt
    42721 aaagcaatgg aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa
    42781 ttattcatat aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc
    42841 accagaatat tcgttgccac ctggacgata tttggctggc tgttttcttg tgcaaaccac
    42901 gctttaacga tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg
    42961 ccatccagtt gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac
    43021 accaccacta atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga
    43081 tggcgcatta agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg
    43141 attaaactca ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact
    43201 tttggcagta aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc
    43261 tgaacaattt tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg
    43321 tcctggagaa aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt
    43381 aacggaacac cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag
    43441 gtatgcagtt tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag
    43501 cgtaaatgcc gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag
    43561 aaattaacta tttcacatat tagcaaccaa ccagcaacat ccttatgaca caaaaataga
    43621 aggtcaatac atcttatctt tcaggattaa aaaatatgac cataccctca gatttgaaca
    43681 tttaccaact gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg
    43741 acatttacca actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg
    43801 aaagccagat cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg
    43861 tcgtggtgcg ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg
    43921 ccatgcgtgt gagttatgca gattgcggca acactcgcgc ctttacctgc ccgtatcacg
    43981 gctggtctta tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc
    44041 cacaagggtt gtgtaaatcc cactgggggc taaacgaagt tccttgtgtg gagagttata
    44101 aagggctgat ttttggcaac tgggatacca gcgcaccggg cctgcgtgat tacctgggtg
    44161 acattgcctg gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg
    44221 gcggcgtaca aaagtgggtg atcaactgca actggaaatt cccggcagag cagttcgcca
    44281 gtgaccagta tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag
    44341 atgatggcag cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg
    44401 ccaaagatgc cctgcaattt ggtcaggatg gtcacggcag cggcttcttc tttactgaaa
    44461 aaccagatgc taatgtctgg gtcgatggcg cagtttccag ctattaccgc gaaacctatg
    44521 ccgaagctga acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata
    44581 ttttcccgac actttcctgg ctcaacggca ctgccacact ccgcgtctgg catccgcgcg
    44641 gccctgatca agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag
    44701 ttaaagccgc ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc
    44761 aggatgactc ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca
    44821 acagcaaact gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc
    44881 ctggcattac taactatatc ttttcagaaa ctgctgctcg tggaatgtac caacgctggg
    44941 ccgatcttct gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc
    45001 aggaggtgat gaaatgagtg cgcaagtttc actagagtta catcaccgca ttagccagtt
    45061 tctctttcac gaagccagct tactggacga ctggaaattt cgtgactggc tggcacagct
    45121 cgacgaagag atttgttaca ccatgcgcac cacggtgaac gcgcaaacgc gcgaccgccg
    45181 caaaggtgtt cagccgccga caacctggat ttttaatgac accaaagacc agctggagcg
    45241 gcgaatcgcc cgtctggaaa cgggcatggc atgggcagaa gagccgccgt cacgcacccg
    45301 ccacttaatc agcaactgcc agataagcga aaccgacatc ccaaacgtat ttgctgttcg
    45361 ggtgaattat ctgctttatc gggcacaaaa agagcgcgat gaaacgttct atgtcgggac
    45421 tcgttttgac aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat
    45481 cgtcctggat caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga
    45541 atttatgcgt gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg
    45601 cccgtcatcg ccttgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc
    45661 catggtaatg cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg
    45721 ctacacgccg ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat
    45781 ccgctcacca cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag
    45841 gcgcagccat gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat
    45901 tagggctggc gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac
    45961 tgtcggcggc gaaagtcgcc agtctgcgtc agcgatttgg tgaacatatt ctggcggtgg
    46021 aaggcaacgt gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt
    46081 ccggcaagct ggattgtttt atcggcaatg cgggcatctg ggatcacaat gcctcactgg
    46141 ttaatactcc cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac
    46201 tcggttacct gctgggtgca aaagcctgcg ctccggcgtt aatcgccggt gaaggcagca
    46261 tgattttcac actatcaaat gccgcctggt atcccggcgg cggtggcccg ctgtacaccg
    46321 ccagtaaaca tgccgcaacc gggcttattc gccaactggc ttatgaactg gctccgaaag
    46381 tacgggtgaa tggcgtcggc ccttgtggta tggccagcga cctgcgcggc ccacaggcgc
    46441 tcgggcaaag tgaaacctca ataatgcagt ctctgacgcc ggagaaaatt gccgccattt
    46501 taccgctgca atttttcccg caaccggctg attttacggg tccgtatgtg atgttggcat
    46561 cgcggcgcaa taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcaa
    46621 ttcgcggcat tcgccacgta gcggctgggt tggatcttta aggaagtacg atgaaagaaa
    46681 aaacgatcat tattgtcggg ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc
    46741 agcaagggtt caccggtgag ctgcatctgt tttccgatga gcaacatctt ccttatgaac
    46801 gccctccgct ctcgaaatcc atgttgctgg aagattcccc acagttgcag tctgtgttac
    46861 ccgctcactg gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacat
    46921 tgggccgcga cacacgagag ttagtgttag ccaacggcga aagctggcac tgggatcagc
    46981 tttttatagc aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac
    47041 gctgctttac cctgcgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg
    47101 aacggtcagt cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca
    47161 cgcagcgcag atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg
    47221 caccaccgcc cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc
    47281 tgctcaataa tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa
    47341 gtggcgagac gcttcaggcc gatgtggtga tttacggtat tggtatcagc gccaacgacc
    47401 aactggctcg cgaggccaac cttgatacta ccaatggcat tgtcattgat gaggcttgcc
    47461 gcacctgcga tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg
    47521 gtgcactaca ccgctgcgaa agctgggaaa acgccaataa ccatgcgcaa attgccgctg
    47581 ccgcaatgtt agggctaccg cttccgctac tgccgccgcc gtggttctgg agtgatcagt
    47641 acagtgataa cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca
    47701 acccggaaac ccagaaagcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg
    47761 taacgctgaa tcagggacgt gagattcgct caatccgcaa atggatccag agcggcaaaa
    47821 cgtttgatgc gaaacagctg acagatgaga acatcgcgct taaatcactg taaccaggat
    47881 aattagcgaa tatctcaacg cctggggcgt ggcgaggtac cagagtgtgt attacgttta
    47941 aatcacatta tcttgcaaag ggattggtta tgaactcact acgttatttt gattttggtg
    48001 ctgcccgccc cgttttgtta ttaattgccc gtatcgccgt ggtcctaatt ttcattattt
    48061 ttggttttcc caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgctgggcg
    48121 caccgatgcc gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat
    48181 taatcgtgct tggctttttc acccgtccgc tggcggtact gtttattttc tacacgctgg
    48241 gtacggcggt gattggtcac cattactggg atatgaccgg cgatgccgtc gggccaaata
    48301 tgattaattt ctggaagaat gtcagtatcg ctggcgcgtt cttgctattg gcaattaccg
    48361 ggccgggggc aatttctctc gatcgccgtt aggaaagatg ccggatgcgg cgtgaacgcc
    48421 ttatccggca ttttataaat tacagccact ctacccgcaa cgacatctct gaggttgttg
    48481 attcccccgg cgcaagcgca atgaggtcac ccccttccgg gcgatgatgg tcatccggcg
    48541 catggctcat cggctccaga cagaagaaat caaacgcata tcctttatca aacgccgggt
    48601 cggaaacaaa gatgaaataa cacggtgcag gtggcgtcgt ttctatgatg atggcataac
    48661 cctcctgcgg ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc
    48721 actggcgcgg caacggcgct agctggttaa aatccagttc ttgcggtagc tgctcgcaaa
    48781 actcacccgc cagccactgc tcccgctcca gccagtaacc gctcgcctgt gcctgaatcc
    48841 gcgtttgcgg cgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg
    48901 ccccttgatt ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct
    48961 gacttacccg atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat
    49021 cgctgtgcga gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat
    49081 cccactcaac attcggttgc agttgatact cacgcccctg ccagacaaaa cggttgccgc
    49141 tcacccggtt tgcaaatggc accagcggaa aacaagaggc atcggttgcc acaccgcttt
    49201 ttttaccagg acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgcctt
    49261 ggtcagaaac gcccagcttt agcgacccgt gggataaggt atagatggtc atccgctaac
    49321 tccttaatcc gggaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcgcata
    49381 ggcatcacct gcctgttcca gcgagaagcg atgggtaatg gcgttacgcg gccacagctt
    49441 ccagtcagtc aaatcgtggg cgcatttttc catatggaac agactggtca cccaggagcc
    49501 aataatccgc cgttgatggt gcatcagatc ggcgctgacc tcgaattcca cttttccggt
    49561 ttcaccaatg taaaccaccc gcccccagtc agcggtggat tgcagtgcca gtaagcgacc
    49621 tgcggcatta ccggaacaat caagcgcaac atccgcgcct ccgtgagtga gttcggcgat
    49681 tatctgcggc agaccttcag tggtcgctaa atagccgtga tccatcactc ctaactgttt
    49741 tgccatcgcc agacgttccg gcagcatatc aacgccgata atccgttttg caccgcgacc
    49801 tttcgccagc atcatcccca tcatgccgac tggccccaga ccgaccacca gcacgttatc
    49861 actgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc cgcaactgat
    49921 aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt tttcttccgc
    49981 cagcaagtat tcggcatgac cgccgtcacg ctgccagccg taagccgctt ttccttcgcc
    50041 agtacaagaa ataggaaaac cgcgacggca gttcgggcaa aaaccacagc cagaaatgtg
    50101 atacaccagc acgcggtcgc cctctttaaa atggcggcag ccttgcccca tcgccacaat
    50161 ctgcccgcac ggttcatgac cgttgataaa tccctagtac aacggtttat cggatgccgc
    50221 cgccgtagcg cggtgctggt ggtagatata gtggacatcg cttccgcaaa ttccggagga
    50281 tttcattttg atcagtacct ggttaatccc cggcgtcggc actgcaactt cccgcagatc
    50341 gacggtcgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc cctcattaac
    50401 aatacgatta attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc
    50461 gctttactgc tacgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg
    50521 ccgcgcacca cctgctggaa aaaggagtta atcccgagca gcaccagacc gttaccgatt
    50581 agcgtaatca ccagtacgcc aagcagcgta ccgaacaacg agccgcgacc accggaaagc
    50641 gccgtaccgc cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt tgcggcacct
    50701 gcgttaccgg aaccgaggcg cgccgccaac aaaatgccgg tcaccgccgc taataatccc
    50761 gaaagggtaa agataagaat gcgtacccga cgaacgttaa taccgcacaa ctgcgccgcc
    50821 gtggcattac cgccaacggc aaaaaccgag cgcccgaagg cggttttgcg gctaatgaac
    50881 acaaacagcg caaacagcac catcatgatc agcgcggata ccggcacacc gagaaattgt
    50941 ccgcccagcc agtccagcac ctcgttttcg tcaatcggca ctggcagagc gttggtcata
    51001 aacaagccca ttccgcgcag ggcgctccac agccccagcg tggccacgaa acttggcacg
    51061 ttaaacacgc cacgcagcac cccggcgagc gttcccatca gcgcgcctaa cagcaacacc
    51121 agcagacaag caatcgccaa cggaacttca aattgcagca aaaatgccag gcataccgag
    51181 acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat aatcagcgtc
    51241 atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc
    51301 aatgagataa agccaggcgc gttcagggag aagaccagat aaagaatggc aatcaccacc
    51361 agcaaaccga tctcattaat atggtgactg acaaattgtt tgagcgagac gctcttgccc
    51421 tgcggcaatg gtaatgacga agcagacatc ttcaggttcc tcggtaaaat tcatcagtgc
    51481 acagacagaa tggcggacat cagctcatcc acattgaccg gagcgtgaaa ctcctgcgag
    51541 aacgtgccgt gctgtaataa caggatgcgg tcacacacca acggcaactc ctccacttca
    51601 ctggagataa acaccacgct ttttccttcg gcagccagct cacggacaat acggtaaatc
    51661 tgctgtttgg cttcgatatc gacgccgcgc gttggctcgt cgagcaacaa aatctggctg
    51721 gcggcataga cccaacgacc gatcactact ttttgctgat tgccgccaga aagcgtgccg
    51781 atgggcgttt cgctactggc ggccttgacc gtcatccgct gcatcacttc ttcggtcagg
    51841 cggcggatgg tggaccattg cagcactccg ttggcgctga ttttttgccg attggtcagc
    51901 actgtatttt cgtcaacgcc caaccaggga atgatccccg cttctttgcg gttttctggc
    51961 gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcgtgat tttctcgccg
    52021 ttgataacaa tttcgccctg ttcatacgtc tccagcccaa caatcgcctt cagcaattca
    52081 ctgcgccctg cccccagcag accagcaatg ccgagcactt cgccacgacg tagcgtaaag
    52141 ctgatatcct ccagcttggg cttatggcgt aacgcacgga cttccagcac ggcctgatcc
    52201 acaatttcct gaggtgcaac cggcgcaata tcaacgtgat cgcgccctag cattagcgac
    52261 acaatatgat gcgtggaagt gttttcgagc atcacatcgc cagccacctg cccgtcgcgc
    52321 ataacggtgg cactggaggc aatgcggcga atttcttcca tccggtggct gacataaatc
    52381 accgccacgc ccagtgctga catctttttc accgctctga tcaccagttc aacttccgca
    52441 ctcgcaagcg aactggtagg ttcatcaaga atgaccacgc gcggctcgcc cttcatcacc
    52501 cgcgcaattt ccaccagctg cttatgcgcc gggcttagtg ttgaaacaag ttgttcagga
    52561 ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat ttgcaggtaa
    52621 tcaatcatgc cgttgcggcg gggccactga ccgaggcaga ggttttccgc caccgtcagc
    52681 ccttccacca gacttaactc ctgataaacc gcgcgtaccc ccagttcagc ggcacggcga
    52741 gtcagcgtag cttcgtcacc ttccagtcgc gtctcgccaa tccagatttc accgctatcc
    52801 ggacgttcac tgccggtaag cattcgaatg agggtcgatt tgcccgcgcc gtttttgcct
    52861 aacagcgcac gaacctcgcc tttattgagc gtgaagttaa cgttatccaa cgcaacgacg
    52921 ccgggataac gcttatttcc tgccaccact tttgctaccg ggactgcctc tgttgccgtg
    52981 aacataggga cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg ttgccagcca
    53041 ctgtttaccg tcttccgttt tggtatagag atcgataggc acctgaatca ctttttcacc
    53101 gtcggcttgt ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc ccattttctt
    53161 accggaaata tccactaccg ctttcagcac ctgattgttt tccagctcct gagcaatttc
    53221 ggttgtcata tccgaaccga aaacagcaat ttttccggcc tgattttgat tacgtaccgc
    53281 ttttaccgcg ccgagtgtcg caccgcccga ttcccccata atggcgttga gatccggcgt
    53341 ggagataatc agtttttcac caacggaaat cgctttatct aaaacagtcc cttcctgatt
    53401 agcgacaatt tgcgcgccgg gaacgcggga ttttaatact tcttcaaatc ctttacgtcg
    53461 ctgcacacaa acttcaaagg cttcgcaatt gatgacggca attttcggct ggtcaatttt
    53521 attggcaata aaataatcgg cggcagcgtt acccagtttt ttaccaaatt ccagcgggtc
    53581 acccaccaga tacgccgaga catatttatc gacccccttt tgattaatac aggtgttgta
    53641 gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac tgccattttc
    53701 agacactgcc gataaaataa tagcatcgac attacgcgcc acgagggtat caacaaaggt
    53761 actttctttc gaaatatcac cctgggcgtt agtttcaatt aactgtacct gtactgaaga
    53821 atcttttgcc gcatcctgaa ctccctgacg cactccagcg taatatccct gagtatcaag
    53881 gtatattgcg ccaatggtca tttctttatc cgcagctctg gcaaatagtg cgctacctaa
    53941 tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca tagagcctcc
    54001 tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg gcagcgcgtg
    54061 ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac
    54121 ggtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc
    54181 agattcgtat tgcgcaatct cgcctaatcc cgcggccgcc agcatcgcca ccatcaggca
    54241 tttttcttta tgctgctgtt gtaaatcgcc atacagcgac aacaagtcag gctgcgagac
    54301 ggcaaagaaa tccgcttcga tactggtttt cgccatctct tgcgcccact gtttcatttc
    54361 actaaacagt tgctgtgcgg tgtgttgttc gcccagtaat cgcagcgcca ttccttgcca
    54421 gaagaggtaa tcaaccggct gatcgttgta atagctgtgg atgttaatgg tgcgatcgcc
    54481 ggtcgccgcc agatgtaaac agcgcgtcgc ttcagtttca tcgccctggg ctttggcgca
    54541 tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa
    54601 attctccgga taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc
    54661 atcaagatgc tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc
    54721 ttccccgcct tcccacgggt ggaatttgcg cgtggcgaga atgtccgccg ctttgtctgc
    54781 ctgccccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc
    54841 aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggttc cacttagctt
    54901 atcaagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt
    54961 atcaagataa cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa
    55021 cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc ataaggcaat
    55081 ggcttcgttg tagctacgtt tgttgtagta gaagcaggcc agtaaatggc gagcaaacca
    55141 gcactcttca atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa
    55201 ctgcgggaag acatcaatgg cttttgcgac cagttcgcca cgttcggctt tcggcagcaa
    55261 gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcattca gcatctctgc
    55321 cgcgagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc
    55381 gcgtccctga cacagaccgc gccactgcgc gagagctgat tcgctacgac catcgaacca
    55441 gttcaggcac cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcaa
    55501 acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc
    55561 ctgattgatt gggcaggcgc gaagactttg ttggcaaaaa tccagacccg cgtcgaagtt
    55621 accattacgc gccgccagtc gtgccagacc ataatagcct ccggctttac tgttgccgct
    55681 ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc
    55741 actggcgcga atcaaactcg cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg
    55801 tttcagagcc tgagtggcat acgccaccgc ttgcgggaaa tctgcgcggt tatactccag
    55861 catcgccagc gccagattac agcggtaatc cagcggatcc agcgccacgc cgcgcaggta
    55921 gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca
    55981 ggcttcatct gtactggtaa tgtcttgtgc tgacagtggc gctttggcga cgtccggcaa
    56041 cggcaactct tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcaga
    56101 gagctcaatg gtcagtcttt caggattgat accgtgtaac acgccctgga tggcggtcgc
    56161 tggtgtcagg gccacggcat catcgagcaa cgcgtcgcat ttgccgattt cgcggatcgc
    56221 caggcggtat ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgctcact
    56281 acgctggagt ttgattaccg catcgcggga ggcgttttgc accatgccca gagaatggta
    56341 aggcaggaaa aactgctcaa aacgcttctc ttcgtaagca tcaagccggg taaaatcagg
    56401 ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat tatcagttag
    56461 gctcttatcc cacgcctggc caaattcact gtgtccccag ctccactgct ttttacctgg
    56521 cgcaatatgg tggttggcaa cgtgtagcaa accgccatct tcatcgtgac accacgcgcc
    56581 aacaaaatcg tactgtgatt tttcagccat atatgaggtt ggaacgggca catttttata
    56641 gcgagaaatg tccactccag cggagtagtc cactttgtag taagtgccgg tggcgatagg
    56701 gaaagcggag acggcccgtt tgccgtgatc aaacaccgcc gttacatccg gcggaaagac
    56761 gctctgatgc ccttcacccc ctttcactgc cgggttggcc caccacaaga aatgacgcgg
    56821 cgtggcgttg ccgttataga cgcggctggc gatttccagc gccgcccggt cagggcgcag
    56881 ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccta cccacaccgt
    56941 ctgtgcgccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg
    57001 atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca gcagccccac
    57061 cagcgcaggt ttaatgactt cattgtgata gacaaaatcg cgctgtttca ctttatccca
    57121 tgcgcgatgc acccgaccgc ccaattccgg caggatcatc actttgatgt agtcgttttc
    57181 cagccacacc gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc
    57241 gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg
    57301 tatatcctgc ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac
    57361 tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg
    57421 tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta
    57481 gtgaagatga gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt
    57541 ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa
    57601 atttcgattc cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg
    57661 aatatcgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg
    57721 gaaggtgact ggacgctgtg tctgaacgtg acgcccacca gtattgagtg tcaggttgct
    57781 aatgcttgtt taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg
    57841 caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac
    57901 cgcaccatca atctggcgct ggcaatccac ggtcaggttg atccagtgac tggagtgtcg
    57961 caaaccatgc cgcaagcgcc gtggacaacg ccggttgagg tgaagtatct gctggaagag
    58021 aagctgggca ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggaaaaa
    58081 tggcaaaata attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc
    58141 tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacagatt
    58201 ggtcacacca tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta cggctgcctg
    58261 gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc gggtatggct aaaatcacaa
    58321 ccggttaata ctcagcttga tcctgaaaaa ctgactacag cgcagttaat cgctgcctgg
    58381 caaagtggag aaccgtggat caccagctgg gttgatcgct ctgccaatgc cattggtttg
    58441 agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt
    58501 tgtgcctttg gtgagcactg gcttaatact attattcgcc agacaggatt taacccgttc
    58561 gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc
    58621 gcacaacagg tgctgggaat tggctatttg tatgttgaga cgcagttacg acagatttga
    58681 tggcgcgata acgtagaaag gcttcccgaa ggaagccttt ttcaacagat taaccgtttc
    58741 gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt gatgcgctcg
    58801 ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc cagttctttc
    58861 gcttctactt ctttgaagcc gcgacgcgta attgccggag tacccacgcg aataccggag
    58921 gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt gatgttagca
    58981 cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcgaccag atcaaccagg
    59041 aacagatggt tatcagtacc gccggaaacc actttgtagc cgcgttccag gaacacttct
    59101 accatcgctt tagcgttttt agcgacctgc tgctggtaag ttttgaactc aggctccatc
    59161 gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc gccctgacca
    59221 ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc tttcgccagg
    59281 atcagaccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac aacgtgagca
    59341 tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg cgccatatca
    59401 acgaacaggt aagcgccgat gctgtcagcg atttcacgca ttttcgccca gtccaccacg
    59461 ccggaatatg cagagaagcc accgataatc attttcggtt tgtgttcttt ggcttgtttt
    59521 tccagatcgg cgtagtcgat atgaccggta gcatcaatac cgtaaggaac aatgttgtac
    59581 agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg cgccaggttc
    59641 atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa gttagcctgg
    59701 gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt cgcacgatcg
    59761 atcgccagtt gttcaacgat atcaacatac tcgcaaccac cgtagtagcg tttgcccgga
    59821 taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac gcgcgggctg
    59881 gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt ttcctgctcc
    59941 atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt taacatccgc
    60001 atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct gttggacaac
    60061 ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga atttacgcaa
    60121 acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac cggaaacgga
    60181 tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct gaaaaacacc
    60241 aaagaaccat ttacattgca gggctatttt ttataagatg tatttgagat acatcaatta
    60301 agatgcaaaa aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca
    60361 ccatcccttt actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt
    60421 ttactcataa cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc
    60481 aacgtgaagc cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg
    60541 cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac cagtttccag atcaaaccgg
    60601 aacagtacaa catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg
    60661 ggcaggaagt gctggacgcg tggggtaaag cctatggtgt actggctaat gtatttatca
    60721 atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc cggtggctgg gaaggtactc
    60781 gcgatttccg cattgtggct aaaacacctc gcagcgcgct tatcaccagc ttcgaactgg
    60841 agccggtcga cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc
    60901 tgaagccgga aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg
    60961 atggcaaagg ctatcgtatt gcggtgaaac gcgaagaggg tgggcaggta tccaactggt
    61021 tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct
    61081 ttatggctgt cgcagatgac acaccagtga cgttaatctc tgccggtgtt ggtcaaacgc
    61141 caatgctggc aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt
    61201 tccatgcggc agaaaatggc gatgttcacg cctttgccga tgaagttaag gaactggggc
    61261 agtcactgcc gcgctttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg
    61321 ctaaaggtca gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca
    61381 gcgatccgac aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttgccgcga
    61441 aacagttagt ggatctgggc gtgaagcagg aaaacattca ttacgaatgc tttggcccgc
    61501 ataaggtgct gtgatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt
    61561 aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat gacccgtgcc acgtcaaaga
    61621 cgaagatttt accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcgacac
    61681 aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca
    61741 ccatatactc cgcgccgcgg tacagctcgg tatggccttt ctggcggcca aaacctttca
    61801 cctcagtgac cgtcatgccg gtaatgccga cttcagccag tgcttcgcgg acatcgtcca
    61861 gcttgaaggg tttaataatc gcatcaatct ttttcatgct attccttgaa aaggtcgcct
    61921 gtcttttgat ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat
    61981 ccagctcgtg tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg
    62041 ccgcgtgggt gacgttgcct ttggtaattt gcagcagctt acgcaaatag ttgagttcaa
    62101 actgattacg tgcctcaaca aaggttggca gcgccgtatt ttcaccctcc agcgcctgct
    62161 ctactaatgc atcgctaatc accggagatg aggtcagcgc cacgcactgt tcaatcacgt
    62221 tgaccaactg gcgcacatta cccggccagc tcgcggtcat caggcgtttc atcgcatcgg
    62281 tggaaaacgc gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg
    62341 ccagtagcgg aatgtcttct gtgcgctccg ccagtgccgg aattttcagg ctgacaacgt
    62401 tgaggcggta atagagatct tcgcggaatt ccccgcgcgc catcgctttt ggcagatcac
    62461 ggtgagtggc agaaataatc cgcacattga tatcaatatc gcggttactg cccagcgggc
    62521 gcactttacg ctcctgcaac acgcgcaaca gtttgacctg caacggcgca ggcatatcgc
    62581 caatctcatc gagaaatagc gttccgcctt ccgccgcctg gaataaacct tcgcgattgc
    62641 tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag ctccgactcc agcaattgct
    62701 caggcaatgc accacagtta atagcaataa atggtttgct attgcgcggg ctggcgttgt
    62761 ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg gccattaatc aaaacgctga
    62821 cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg
    62881 tgacaatcgc ctcgcgccag cgttcgtcgg tggctggcgc ggattgttcc agcgcatcat
    62941 caattgcctg atatagcgcg tctttatcaa caggcttggt gaggaaacta aaaacgccct
    63001 gctgtgttgc agcaacggca tcgggaatag aaccatgcgc ggtaagaata attaccggca
    63061 ttcccggctg cactttctgg atttcagcaa acagctgcat accgtccatt tcatccatcc
    63121 gcaggtcgct gatgactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcgc
    63181 cactttccgc cgtgaccacg ctgtagcctt cgctggtcag gcgcaggcca agcagtttca
    63241 gcaatcccgg atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg
    63301 acctcatggg tggatggcgc gggcttttca ctctcgtgtg gcgtatccgg cgagaaattt
    63361 ccagccggtt tgcgggtcga gagctggcgt tcaatatcag tcaggttttc cagcttgcgg
    63421 gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg
    63481 ctgtcgctgg actgctggag tttactgtag cgttggcgct cttccgccag ttgcagttgc
    63541 agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa cctgtgccgg gatctcggta
    63601 cttaacgctt caatacgcgc gaccagctgg cggcgctcat atggcgtaat tttggcgtcg
    63661 gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc cgtcgtcata ttggcgagct
    63721 tgctgacgcg actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa
    63781 agcggattgg tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa
    63841 agatagtcag ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc
    63901 ttattgtgat tctgcacgca acctagcaat gccagacatg gcagccctgc cagccacagc
    63961 cgtcggggca ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc
    64021 agtgggcgta attgttgcag acagtttgga catggacagc gcggagaaac cggagcgtac
    64081 atgtcgtacg tgaggatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg
    64141 atgggatagg ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac
    64201 aaacgtcttg cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat
    64261 ccctggcaat gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc
    64321 cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat
    64381 tgatgacatc aatataaacc cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg
    64441 attcagcccc gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca
    64501 ttggctccgc caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc
    64561 gtgcgggcag gctatgagca gaaactactg tctccaccag cggtgctaac tcaacattat
    64621 ctagttccac cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt
    64681 tttgcaaatt gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa
    64741 gcggcccgac aacctgatca gccagtaatt cagtgccctc gcgcatactc gccagtggcg
    64801 tttttaattc atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca
    64861 ggcgctcact taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga
    64921 acgaaacgct attgcccaga gaacgccctt cccccaaccg attgatcatc cgttcaatat
    64981 ttttcaccgg cccgataatc atccgcgtga aaagcagtac cattaccaga ctcaccagaa
    65041 atagcaccag cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt
    65101 gctgcccacg agagaacact actgtgcgcg tggcctgtac catttcggta ttggcactgg
    65161 caaaggcttc cagacgcgcg gcagcagcag catcgggacc actgttgttg cactgaagtt
    65221 gggccagatt gttcaagtcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc
    65281 ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa
    65341 gcgtagggtc atccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt
    65401 tggtcattgc ttcgctgcgc cgggcatcga taagcgtggt gcggttgacc agcgccgcct
    65461 ggtcgctaag cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggcagca
    65521 gaatcagcaa aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc
    65581 gtttcaaggt gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca
    65641 gatacaacaa agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga
    65701 cgtttcgccc gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt
    65761 gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa
    65821 tgagccactg attactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt
    65881 actgattata cgttgctttg aggggtttat ggcttccccg ctatttgaat gtaaatcagc
    65941 cactgtgtcg ctaaaaagag acaacttaag aaaaacttat tagattacaa taaaatcaat
    66001 catttaagtg tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac
    66061 aaaaaagctc ccgaagttgg gagcttataa tagcggttgt atttaatgcc ggatgcggcg
    66121 tgaatgcctc atccgggctg caaaaccaat gagtttgacg gcttacccca actgcttacg
    66181 tgcattgcgg aaaatgcgca tccatgggcc atcctcgccc cagttttcag gatgccagga
    66241 gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc ataatggtga ctcgaccgct
    66301 ttcagtcgtg accgccgtaa taccgttcgg agaaccgttc gggttagccg ggtaggtttc
    66361 agtgactttg ccgaagttat cgacatagcg cagtgccacc agccctttgc tttccagtgc
    66421 cgccagatgc gccgcatcac gcacttccac gcgcccttca ccatgagaga cagcaatcgg
    66481 catttgcgag cccaccatcc cctgcaacag cagcgacggg ctttgggtca cttcaaccaa
    66541 gctgaaacgc gcttcaaagc gatcggaggt attgcgcaca aaacgtggcc acaactcact
    66601 acctgggatc agttcacgca gattagacat catctggcaa ccgttacata cccccagcgc
    66661 cagcgtttgc ggacggtgga agaaggttgc aaactcatcg cgtacacggt cattgaacag
    66721 gatagacttc gcccaacctt caccggcacc cagcacatca ccgtaggaga aaccaccgca
    66781 cgcgaccagg gcgtggaaat cctccaggcc cgtgcgtcca gccagcaggt cgctcatatg
    66841 cacgtcgata gcatcgaagc ctgcacggtg gaaagctgcc gccatttcaa catgcgagtt
    66901 cacgccctgc tcacgcagca cagcaacttt cggacgtgcg ccagtggcaa tatacggtgc
    66961 tgccacatct tcgttgatat caaacgacag tttcacattc aggcccggat cggcgtcgtt
    67021 tgatttcgcc tgatgctcct gatcggcaca ctccgggttg tcacgcaggc gctgcatctg
    67081 ccaggtagtt tctgcccacc agacacgcaa cgtagtgcgg ctttcgctga atacagtctg
    67141 cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc tgcccaacat aatggacgca
    67201 atcagcaagc ccatgctgtg ccagtacgga ctcgaccgct tcacggtcag cggcacgaac
    67261 ctgaatgacc gcacccagtt cttcgttaaa caacgccgcc aggcgatcgt cacccagagt
    67321 ggcgatatcc gcgtcaatgc cgcaatgacc agcaaaggcc atttccgcca gcgttaccag
    67381 cagaccgcca tcagagcggt cgtgatacgc cagcaactta cgctgtgcaa ccagcgcctg
    67441 aatcgcgtca tagaagcctt tcagttgcgc gacatcgcgt acatctgccg gtttgtcgcc
    67501 aagctgacga taaacctgcg ccagcgccgt tgcgcccagc gcgttattgc ctttgcccaa
    67561 atcaatcagc agcagtgcgt tatcttcggt agaaagctgc ggcgtgatgg tgtgacgtac
    67621 atcttccacg cgggcaaatg cagaaatcac cagcgacagc ggcgacgtca tttcgcgctc
    67681 ttcgttacct tcctgccagc gggttttcat cgacatagag tctttaccca ccgggatcgt
    67741 caggcccagc gccggacaaa gctcttcgcc cacggcttta acggcttcat acaggcccgc
    67801 atcttcacca gggtggcctg ccgccgccat ccagttggcg gaaagtttaa tgcgtttgat
    67861 atcgccaatc tgtgttgcgg caatattggt taacgcttca ccgaccgcca gacgggcaga
    67921 ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca atcgccatcg cttcaccgta
    67981 gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg accggcacct gccacggccc
    68041 caccatctga tcgcgcgcca ccatgccggt tacgctgcgg tcaccgatgg tcaccaggaa
    68101 agttttctcc gccacggtcg gcagatgcag tacgcgtttc accgcgtctg caatggtgat
    68161 cccttcacgg accagcgcat cgcccttcgc tttcagcgtt tgtacatcgc gggtcatctt
    68221 cggcgtttta ccaagcagga cgtccagcgg cagatcgatc ggctgattat caaaatgacg
    68281 atcgtgcaga gaaagatgca gttcttcggt cgcttcacca atcaccgcgt agggtgcgcg
    68341 ctcacgctta cacagttcgt caaacagcgg taattgatcg gcagcaaccg ccagcacgta
    68401 gcgttcctgg gattcgttac accagatttc cagcgggctc atgcccggtt cgtcgcttag
    68461 aatatcgcgc agttcaaatt taccgccgcg cccgccgtcg ctcaccagtt ccggcatcgc
    68521 gttagaaaga ccgccagcgc cgacgtcgtg gataaacagg attgggttgg catcaccaag
    68581 ctgccagcaa cggtcgatca cttcctggca gcgacgctcc atctccgggt tgtcgcgctg
    68641 tacggaagca aagtcgaggt cggcatcaga ctgacctgac gccatggaag acgccgctcc
    68701 gccgccaaga ccgatgttca ttgccgggcc gccgagaacg accagtttcg caccgacgtt
    68761 gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg atcccgcccg ccagcatgat
    68821 cggtttgtga taaccgcgca gctcttcgcc gttgtggctg ttcacttttt cttcataagt
    68881 acggaagtag ccattcagtg ccggacgacc aaattcgttg ttaaacgccg cgccgcccag
    68941 cgggccttcg gtcatgatgt ccagcgcggt gacaatgcgc tcaggcttac cgaaatcttc
    69001 ttcccacggc tgttcgaagc caggaattcg caggttggaa acggagaaac caaccaaacc
    69061 cgctttcggc ttcgcgccgc gcccggtggc accttcatcg cggatttcac cgccggaacc
    69121 ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg ttgtgagttt cgactttcat
    69181 cagaatatgc gccggttcct gatggaaatc gtagcggccc gtttcgtggt cagcaaagta
    69241 gcggcccact tcagaacctt ccattacggc ggcgttatct ttataagcag agagaacgtg
    69301 atctggcgtg gtttcgaaag tatttttgat catcttgaac agcgatttcg gctgctgttc
    69361 accgtcgata atccagtcgg cattaaaaat tttgtggcgg cagtgctcag agttcgcctg
    69421 agcgaacata tacagctcga tgtcgttcgg gttacgacca agctttgtga aagcatcctg
    69481 cagatagtca atttcatctt ccgccagagc caggccaaga cgcaggttag cgtcgatcag
    69541 cgcctgacgg ccctgcccca gcaaatcaac gctggtaacc ggagtcggtt gatggtgggc
    69601 aaacaactgc tctgcatcat ctaaagcaaa aaagaccgtt tccatcatgc ggtcgtgcag
    69661 ttcagcggta acctgctgcc attgttcatt ggtcagcgta ccggcttcta tatagtaagc
    69721 aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag ttatgagcaa tatcggtcgc
    69781 tttcgaagac cagggagaga tggtgccagg acgcggggtc accagcagga gtttgccttg
    69841 cggggcgtgg ctggcgagtg ccgggccata tttcagcagg cgttcaagtt gtgcgtgctc
    69901 atcatcgttt aacggcgcat tgaggtcagc aaaatggaca tactcggcgt aaatattgtg
    69961 aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg ttgattcgga atgccgacag
    70021 tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg tcttctaagc tttcggtgta
    70081 cccaaggggg gaaacgggcg tcattataaa gaatctgatg cgctgacgaa accgtttgcg
    70141 tggaaataaa atcaccatcg tgaattagca acgcgtgccg ccaatggctg taataagttg
    70201 ccatctggcg caggtttacg caaaatgccg ctcatttatg agtaaacctt tcactattat
    70261 tacatttttt caagctggga cgcgcacgac acagagaatt aactaattga aaaaattaaa
    70321 gattaattat ctgttcatcg gcattctggc actgctgctc gcggtcgctc tctggccatc
    70381 cattccctgg tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt
    70441 gcgtgtgagc accattcata ctcccctgac gtataacgaa atcaacggga aaccttttgg
    70501 cctggattac gaactggcga aacagtttgc cgattacctc ggcgtaaaac tgaaagtgac
    70561 cgtgcggcag aatatcagcc agctgtttga cgacctcgat aatggtaacg ccgacctgct
    70621 ggcggcagga cttgtctata acagtgagcg ggtaaaaaat tatcagcctg gccctaccta
    70681 ttattccgtg tcacaacaac tggtttataa agtgggtcag tatcgcccac gtacgctggg
    70741 caacctgacg gcggagcaac tcaccgttgc accgggtcat gtggtggtta acgatctcca
    70801 gaccctgaaa gaaacaaaat tcccggaatt aagctggaag gtagacgaca aaaaaggctc
    70861 tgcggaatta atggaagatg tcatcgaagg aaaactcgat tacaccattg ctgattctgt
    70921 cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg atatcaccga
    70981 tgaacaaccg gtgacctggt ttagcccgtt agatggcgat aatacccttt ccgccgccct
    71041 gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg aagagaaata
    71101 cctggggcat ggcgatgatt ttgattacgt cgatacgcgc acatttttac gcgccgtcga
    71161 tgcggtactg ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa ttgactggcg
    71221 tttgctggcc gctattgctt atcaggaatc gcactgggat gcacaggcca cttcaccgac
    71281 gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg caaagcctcg gcattacgga
    71341 tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg atatgatgag
    71401 taaagtgccg gaaagtgtgc cggagaacga gcggatctgg tttgccctcg ctgcgtacaa
    71461 tatgggctat gcgcatatgc aggatgcccg cgccctgacg gcaaaaacca aagggaatcc
    71521 tgacagttgg gctgacgtaa aacagcgtct gcctttactt agccagaaac cctattacag
    71581 caagctgact tacggctacg ctcgtgggca tgaagcctac gcttatgtcg aaaatattcg
    71641 taagtatcag attagcctgg tgggttatct gcaagagaaa gagaagcagg ctacagaagc
    71701 ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct acggagttgg gcaaagagaa
    71761 atttcctttt ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc attctccctc
    71821 tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg aggattgcgc
    71881 ttttttctgc gctttaattt cctggcggcg catgcgaaag aagtcactga gcaacgccgc
    71941 gcactcatcc gccagtattc cttccgtaat ttccactcgg tgattcatac ccggatgatg
    72001 cagcacatcc attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac caaagaccac
    72061 gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt acacatggtt caagcgtgac
    72121 atacaacgtg gcgtcgatca gacgataatt ttgcatcacc agaccgccct gcctcagggc
    72181 catgatttct gcatgtgcgg tgggatcatg tcgaccaatc ggacggttcc agccttcgcc
    72241 aataacacga ttgttatgca ctaataccgc gccgaccggt acttcccgct catcccaggc
    72301 acgcttcgcc agcgtcattg catgacgcat ccagtattcg tggctaaatt cgacttcaga
    72361 caaaaagaaa actccggtta taaaagcgcg gcgcattata cacggacgct atgctttact
    72421 ctattccagt tgctggagtt caccgcgcgg ggttactcgc cagcgatgct ggcaaaagta
    72481 aagcagcggg ttgtcctgat tactgtcgct atagccactg tacagccgca gcggcgtgcc
    72541 gattttgcgc tccagttgcg cgaccttttc atgcccgagg caacgcatcg tcaataccca
    72601 accaccatag ccacgctgaa tttggctggc gataagattc acccgcggca gccagggtgt
    72661 atcgaaatag accgcttcaa ccagcggctg tggagaaccg gtaatcaacc agatatcagc
    72721 atcggaactt aacaggtagg tggttaatcg ctcctgaacc agcggaaagg cggtaacatt
    72781 gtcacgaaac cagcgtacga aatcggcctg caacgcctgt aaacgtgctt cgctgtgacc
    72841 aaaggtgcat ccccacagaa gcagactcat cggccagcgt gccgcacgac cttttatcaa
    72901 taacgcaatg gctataatcg gcaacaacgg caggacaagt aacgcattca gcggttggcg
    72961 acgcagtaaa tagcgcagaa aactgccgaa catatcctgc tgatgcaatg ttccatctaa
    73021 gtcaaaaaac accacacgac gctcgtgagt tgccaaacca tactcctctg gactgaaaca
    73081 tcctgattaa ttttcgctac tacatagcct aacagataga tcatcacttt tccggcaaca
    73141 atcgaccgca tcatggctaa ctggaatttt taattcatgg caattagcgg caatggaata
    73201 taaaattcac tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca tcgcgaggaa
    73261 agatatgaac ggcttacttc gtatccgtca gcgttaccag gggcttgccc agagtgataa
    73321 aaaactggcg gattatctgc tgctacaacc tgatacggcg cgccatttaa gctctcagca
    73381 actggccaac gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg
    73441 ctataaaggt tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa gccagccgga
    73501 atcgccctca gtgcccattc ataaccaaat ccgcggtgat gatccgttac ggctggtcgg
    73561 cgaaaaactg attaaagaaa atactgccgc tatgtacgca acgctaaacg ttaatagtga
    73621 agagaaactg catgaatgcg taacaatgtt gcgctctgcg cggcggataa ttctgaccgg
    73681 tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga agattggctt
    73741 caatgctgcc gcagtgcgcg atatgcatgc gctactcgct accgtacagg cgtcgtcccc
    73801 tgacgatctg ttattagcca tttcttacac cggtgtacga cgcgagttaa acctggcggc
    73861 agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt accggcttta ctcccaatgc
    73921 cctgcaacag cgtgcttctc attgcctgta taccattgcc gaagaacaag cgacaaacag
    73981 tgcttcaatc tctgcttgtc acgcgcaggg aatgttaacg gatttgctgt tcattgcgct
    74041 gattcagcag gatctggaac tggcaccaga acgtattcgt catagtgaag cgctggtgaa
    74101 aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga
    74161 gtttccttat ggcgctgtta atcactaaaa aatgcatcaa ttgtgatatg tgtgaacccg
    74221 aatgcccgaa tgaggcgatt tcaatgggag atcatatcta tgagattaac agcgataagt
    74281 gtaccgaatg cgtagggcac tacgagacac caacctgcca gaaggtgtgc ccgatcccca
    74341 atactattgt gaaagatccg caacatgtcg agacagaaga acagttgtgg gataaatttg
    74401 tgctgatgca ccacgcggat aaaatttaat attctactct ggaagtagag tattaattat
    74461 tttactggga agccttaacg ccattatatt tatttaattg atgacattag cataataatt
    74521 cactaagtta atttatatag tatctgccca gacacttatt tatagttatt aaaggcgcgt
    74581 ccgattggtt caccggacgc accttaagta cgtttccttg tgttataaga acagaaggat
    74641 cagctgtaaa acagcaatga tgattttgat gacccgttta atcaggtatc ggcaatcagt
    74701 cattcgtttt tccttaaaca aggaatgcag ccgttcagat tagcccttac atctccccaa
    74761 aactgaacgt gcgagttatt ggaatcgagt gctgcactcc acaccagagc tttgacgaca
    74821 ccactcgttt caatggggga attcagtggc atggtgtaaa gcacagcaaa attgccaata
    74881 acgaaaccga ttttaatgta cttatcaaaa agcggtcacg ttaatctttt catcaatgtc
    74941 gatttcttcg taaaggctcg cgttttttat gtcagagatt gcaagttgct tgtacaggtc
    75001 aacgaaattg atccataatc attttcgttg gaatcgtgtg ctgcacaaaa ttaaagttaa
    75061 aaagtaaaac ccccgttcct taccagttcg ggggttttac tttataaaga gaacggtatt
    75121 atttttaact ttcaataatt accgtggcac aagcatagtg gcgctcatcg gccagcgtca
    75181 catgcatatt tgcaacgccc agcttttccg ccagttttaa tgcctcgccc cacagtcgca
    75241 gccgtggctt accaagctca tcgttaaata cttcaaactg attaaacgcc agaccattgc
    75301 ggatcccggt gccaaacgct tttgctgcgg cttcttttac ggcaaagcgt ttcgccagaa
    75361 aacgcaccgg ctggtggtgc gttttccaaa tttcccattc gttatcgctt aatacgcggc
    75421 gtgccaggcg ctcaccggat cgggcgatca ctgcttctat gcgagcgatc tctacgatat
    75481 ccgtgcctaa acctaatatt gccattagcc acgcgcttcc agcatcaggc gcttcatttc
    75541 tgccaccgca tctttcagtc cggtcatcac tgcacgacca ataatggcat gaccgatatt
    75601 cagttcgtgc atctcaggga tggcggcaat ggcttttacg ttgtgatagg tcagaccgtg
    75661 tccggcgtta actttcaaac cgagactagc ggcaaaggtc gcggctttgg cgatacgcgc
    75721 cagctcttgc gcctgttcgg cgtcagtttt ggcatcagca tagcaaccgg tgtggatctc
    75781 gataaacggt gcgccaacct ctgccgcagc tttgatctgc tcttcatcgg cgtcgataaa
    75841 cagagaaacc tgaatcccgg catccgccag acgtttgcag gcatcgcgca ttttgtcacg
    75901 ctgccctgca acgtccaggc cgccttcggt tgttacttcc tgacgctttt ctggtactag
    75961 gcagcaaaaa tgcggcttcg tctcaaccgc gatcgccagc atctcttcgg tcaccgccat
    76021 ctccagattc atgcgggtat ccagcgtctg acgcaggatg cgcacatcgc ggtcagtaat
    76081 gtggcggcga tcttcacgta aatgcacggt aatgccgtcc gcccccgcct gctcggcaat
    76141 aaacgcggcc tgcaccggat ccgggtaagc ggtaccgcgc gcgttgcgca gcgtagcgat
    76201 atggtcaatg ttgacgccta acagtaattc agccatgaca atcctcatca ttcataatgt
    76261 gttttcaccg ttcgcttagg cataaattgc cggaacagtt ccctgctctt taaaggttta
    76321 ccgccaagat acggcttaag cgccatgcgg gtaaagcgtt tcgcggcgcg cagtgtgtct
    76381 gcgtcaggaa attcccgtgc gtttaacgct tttaactgcc ttccggtgaa cgttttattg
    76441 tcgataacga cgcttgcgat aaaccctttt tcttcgcgat aacgatacgt catggtgtca
    76501 tctaccggct cgccgctacc cgcacaatgg gtaaaattga cgccataacc cagatgcccg
    76561 agcagtgcca gttcaaagcg gcgcagcgcg ggttctggcg taccagtgac ccctgcaaga
    76621 gactgaatgc agtgcaagta atcgaaaaag agttcagaga agcgcgtctc gtattccagt
    76681 acgcgggaga gaagttcgtt gatgtacaga ccgctgtaaa gcgtgatacc gcttaatggc
    76741 agcgccagcg agacagcttc agcactgcgc agcgttttga cttcgccacg cccgccaaaa
    76801 cgtagcaaga gaggggtgaa aggctgtaat gcacctttca gggtagagcg tttagagcgt
    76861 gcgcctttgg caaccagacg cacgcgaccc gattcctccg tgaagacgtc cagcatcagg
    76921 ctggtttcgc tccacgggcg actatgcagg acaaatgcgc gctgccagcc ttccatcgga
    76981 gttactctta aagatcgtca acgtaaccga gactgcgcag tgcgcgttcg tcgtcggccc
    77041 aaccggattt cacttttacc cacagctcaa ggtgaacagg cgcttcaaac atttcctgca
    77101 tgtctttacg cgcttcaatc ccgatggttt tgatcttggc ccctttgttg ccaatgacca
    77161 tcttcttctg cccttcacgc tcaacgagaa tcaaaccgtt gatgtcataa ccaccgcgtt
    77221 cgttagagac gaaacgttcg atctccacgg tcacggagta cggcagttca gcgccgagga
    77281 aacgcatcag tttttcgcgg atgatttcag acgccataaa acgctgtgag cgatcggtga
    77341 tgtaatcttc cgggaagtga tgagtcgctt caggtagatg cttacgcacg attgccgcaa
    77401 tagtggcaac attcagcccg gtttcggcag agattggcac gatatcgagg aagttcatct
    77461 ggcttgccag gaactgcagg tgcggcagca gatcggcttt ctcctgcacg ttgtccactt
    77521 tgttcaccgc gaggattacc ggcgctttgc cttcgcgcag tttgttgagc accatttcgt
    77581 cgtccggcgt ccagcgggtg ccttcaacga caaaaatcac cagctcaaca tcgccaatag
    77641 agctgctcgc cgctttgttc atcaggcggt taatagcgcg tttttcttcc atatgcaggc
    77701 ccggtgtatc gacgtagatc gcctgatacg cgccttcagt atggatcccc acaatgcggt
    77761 gacgagttgt ctgcgccttg cgggaggtga tggagatttt ctgccccagc agtttgttca
    77821 acaatgtgga tttgccaaca ttcggacgtc cgacgatggc aataaatccg cagtaacttt
    77881 tatcgatgct cattccagct ccagtttttt caacgcctgt tcggcggcag cctgctcagc
    77941 cttacgacgg cttgaacctg tgccaaccac cggttcactc aggccgctga cctggcagtg
    78001 gattgtaaat tcctgatcgt gcgcttcgcc acggacctga actaccagat aagtcggcag
    78061 cggcagatga cgaccctgca aatattcttg caagcgcgtt ttcggatctt tttgtttatc
    78121 gcctgggcta atttcgtcca gacgagtttg ataccagttg aggattaatt tctcgacggt
    78181 ttgaatatca ctgtcgagga atacgccacc aattaatgct tcgacggtgt cggcgagaat
    78241 tgactcacga cgaaatccac cgcttttaag ttcacctggt cctaaacgta agcactcgcc
    78301 taactcaaat tcgcgcgcca gttccgccag cgtattgcca cggaccagcg tggcgcgcat
    78361 ccggctcata tcgccttcat ccacacgagg gaaacggtga taaagcgcat tggcgataac
    78421 gtagctcaga atagagtcgc ctaaaaattc taaacgctcg ttatgtttgc tgctggcact
    78481 acgatgagtt aatgcctgct gcaacagttc ctgatgatta aaagtgtagc ccagcttccg
    78541 ttgaagccga ttaattacga tggggttcat gcgataccaa taaataaatg cgtcaacaat
    78601 tcagcacacg aaacagacct gatatacatg gctctgctaa ctgcttcgct gcagtttcag
    78661 tttagcggta tatggggacc aacgctgttt cgtgtgccgt ggcaacctgg aggtgccaac
    78721 cttaaacttc gggggaatat tctatacaca acgacggggg atgtcgttag ccacgggaga
    78781 tttatctcat aaataattca cgttgtcgcc ataacggcga caacgtgaac gaagatgagc
    78841 tattaatgga tgccgccaat gcgacttaag cgcacacctg tcggccattc gccttcttgc
    78901 ttatcgaagc tcatccagat agccgttgcc cgaccgacca gattcgcttc aggcacaaag
    78961 ccccagtaac ggctgtccgc gctgttgtcg cggttgtcgc ccatcatgaa gtattgtccc
    79021 ggaggaacaa tccaggttgc cagttgttgc cctggctgct ggtaatacat ccccacctga
    79081 tcctgcgcaa tcggcactgt cagaatgcgg tgcgtcacat cacccagtgt ctctttacgc
    79141 tcggaaagac gaattccatt ttctttggtt tcgtttttcg gcacttcaaa gaatccgctg
    79201 gtcgcttccc caccattacg gcgtgagaag gtctgaacga aatcgctcgg ttccacgttt
    79261 gagtaggtga ccggcagcgc gttttcacac gcctggccgg aactgcatcc cggttgaatc
    79321 gtcagctctt ttgagaccgg atcgtaagtg actttatcgc ccggtaaacc caccgcgcgc
    79381 ttgatataat caagctttgg atcttccgga tatttaaaga ccacgatatc gccgcgtttc
    79441 ggatgaccgg tttcgatcag cgttttctgg tagataggat ctttaatgcc ataagcaaac
    79501 ttctctacca gaataaaatc accgattaac agagtcggca tcatcgaacc tgacggaatc
    79561 tggaacggtt cataaataaa cgaacgcaca atcaatacga tagccagtac cggaaaaaca
    79621 gaagcaccgg tttccagcca gccaggcttc ggcgcaacct ttttcaacgt tgctttatcc
    79681 agtgagtcac cggcagccgc ctgcgccgct gcctgacgtt cccgccgttt aggtgcgaaa
    79741 aagaatttat ccacgcacca taaaatgccc gtcaccagtg tggcaatcac cagaatcagg
    79801 gcaaacatat tcgccatgcc aactcctaag ggttatttgt tgtctttgcc gacgtgcaga
    79861 atggcgagga acgcttcctg cggcagctcg acgttaccga tctgcttcat gcgtttctta
    79921 ccttctttct gcttctgcag cagctttttc ttacggctga tatcgccgcc ataacattta
    79981 gccagtacgt ttttacgcag ctgtttcacg gtggatcgcg caataatgtg cgtaccaatc
    80041 gctgcctgaa tggcgatatc aaactgctgg cgtgggatca gatctttcat cttctccacc
    80101 aactcgcgac cgcggttttg cgaattatca cggtgggtga tcaacgccag cgcatcaaca
    80161 cgttcaccgt tgattaatac gtctacacgt accatgtcgg acgcctggaa gcgtttgaag
    80221 ttgtaatcca gagacgcata accacgcgag gtagatttca ggcgatcgaa gaagtcgagc
    80281 accacttccg ccatcgggat ctcgtacgtc agcgccacct gattaccgtg gtaaaccata
    80341 ttggtctgca cgccgcgttt ttctacgcac agcgtaataa cgttgccgag atatgcctgc
    80401 ggcagcagca tgtgacactc tgcaatcggc tcgcgcagtt cgtaaatgtt atttaccgca
    80461 ggcagcttgg atgggctgtc gacgtagata acttcccttg acgtggtttc aacttcatac
    80521 actacggtcg gcgcagtggt gatcagatcc agatcgtatt cacgttccag acgctcctgg
    80581 atgatctcca tgtgcagcag gccaaggaag ccgcagcgga aaccaaagcc cagcgcgctg
    80641 gagctttccg gctcatagaa cagtgaggca tcgttcaggc tgagtttacc cagcgcgtca
    80701 cggaaggctt catagtcgtc ggaacttacc gggaacagac cggcgtatac ctgcggtttg
    80761 actttcttaa agccaggcag cgccttttct gccggattac gcgccagcgt taaggtatcg
    80821 ccgactggag cgccgtggat atctttaatc gcacatacga gccagcctac ttcgccacat
    80881 ttcagttcag tgcggtcaac ctgtttcggc gtgaagatgc ccagacggtc ggcgttatag
    80941 gtctgcccgg tactcatgac tttcactttg tcgcccttac gcagggtgcc gtttttaata
    81001 cggataagtg aaacaacgcc caggtagttg tcgaaccatg agtcgataat tagtgcctgc
    81061 aacgggcctt ccggatcgcc ttccggcggc ggaatgtcgc gcaccagacg ttcgagaacg
    81121 tcctgcacgc caacgccggt tttcgctgaa cagcgcaccg cgtcagtggc gtcgatgccg
    81181 acgatatctt caatttcttc cgccacgcgt tcaggatcgg ctgccggcag gtcaatcttg
    81241 ttcagtaccg gcaccacttc gagatccatt tccatggcgg tgtagcagtt tgccagggtt
    81301 tgcgcttcca cgccctgccc ggcgtcgacc accagcaatg caccttcaca ggcagccagc
    81361 gaacgggaaa cttcatagga gaagtctacg tggcccgggg tgtcgataaa gttaagctga
    81421 taggtttcgc cgtcagacgc tttgtagtcc agcgtcacgc tttgcgcttt gatggtaatg
    81481 ccacgctcac gctcaagatc catggaatcg agaacctgcg cctccatttc acggtcagac
    81541 aggccaccgc agatctggat aatacggtca gacagcgtcg atttaccgtg gtcaatgtga
    81601 gctatgatcg aaaagttacg tatattcttc ataaagtatg attattgtgc cttaatgccc
    81661 ggttaaccag gcttttagaa gtcgctgttc tgagcttaac gtctgtatta atagaaacgc
    81721 cgcattctac actacaacat tgaggcgagg aaatgttcat accgtatgga ttgtggtatc
    81781 tggaaacgtc ctcgcatttg ttatgcaaaa tgcaacaaag ccagtgaaat cactggctcg
    81841 cgtcttccga agatgtttca aatcgcacaa ggccaggcgg caatgccacg cttaagatga
    81901 tcggttgcca ttctgcccgg gcagcaaact tacgcgagta gccacgcgca atcaggaacc
    81961 cgccaatacc gccgaggacc gcaccacata atgctgcgac gtcagaagca aagagtagct
    82021 gaaatagcga agcgatgagg aataatccca ccagcggcga catataaacc agtaatgcgg
    82081 agctaagcag gctgccttcg gcgatcccca attccacttt ttgccccggc actaacggct
    82141 catcacaggg tacgacaatg gtatgcgtgg tttgcgggcc aagtttattc aacacgcggc
    82201 taccgcaacc ggcgcgtgaa gcgcagctgc tgcatgaggc tttaacatca caactgacca
    82261 gcgcctgccc gttttgccag gagacgacgg tagcccactc tttgatcatt gcgctgcccc
    82321 gaacttaata ttctcggcaa tgcgtttcgc cgtttgcggc ggcagttcac cgacaatggt
    82381 gatttcggcg ttatcacgta cgcttgtact gacggttctg cgtccggtgc gcaacatctg
    82441 atcggtgctc gatggcgtag cgcggttaac gtttaccgag aagctgaata atccgtcgga
    82501 atagagacgt gattcgatag gcatgttgtc catcgtcggt agcggacgtc gactactgga
    82561 aacttcgcta aaaccctgtg gcaaccaggt tggcgtccag ctgaatttag ctttttcacc
    82621 tacaggaaca gaaagcaacg gcggcaaatt tgccttcgcc agcgtctgca tactgctgct
    82681 gatatcctga ttgacgttaa aagcaatcac gcgaaattgt tccagcgttt caccatcgcg
    82741 atcaagaaga tcaacccgca tcggtaattt cgattcggtg tccatccaca cgatgtagct
    82801 gtagcgtgta ccatctcggg caaccacgcg aatgacttcg caaagacgat cagcaatacg
    82861 cgtgcgcccg acggagataa agtcgtagta aggagaaagg cgtttgaaat cggtatagat
    82921 gagcgatggc agagaatcaa caatgtaatc gccattaagc gtgaacggtt caagtcccgg
    82981 ttcaaaatag ctgatttcat tgccgcgctg taccacttcc cggcgcgggc catccatttg
    83041 caacaattgt gcaagaggac ggttatcgag gcgtgcatgt cgataacgca gagactcaac
    83101 accctgttta ttgatgctga tgaatgacag ctcgtaattc agtgactgac tggccaggtt
    83161 catctgctgt aataacgccc cggacgcggg agtggccgag gcgttagcag agaataacag
    83221 gctacctgtc actaatgaca tggcaaacca aagttgcttc attactgcga ttgcgttcct
    83281 aaagtttgaa ttcctggcac ctgtacagcg gcttgctggg tttgcgcctg ctcaaactga
    83341 agctgttcag agtggagtcg gcgttgcagt tcgtaatcct gcaacattgc attaatgcga
    83401 cgacgctgct gctgttgacc attatttgcg gtcgcttcag aaggtactcc caggctaacc
    83461 gggctggctt tacccatcat cggcagtgta ttaaataccg gcgtttcggg ctgctgggac
    83521 gtttcagatt gtccattata gtgctggacg ccaacgataa ctgcaagcga tacgcatgcg
    83581 gctacgccca tttgggtaag ctgtgccgcc cacggacgta ctttctgcca gaatggcatt
    83641 ttctgccatt catgcggcgc aggctgggct tccgggatca atgtcgccgg ttgacgtact
    83701 ggctcttctt caatggcggc catcacgcgt gaagagatat cgaaatggag cacctcggga
    83761 gtatcacccc gcattgagtc acggattaag tgatagcttt cccaggtttt ctgcatttct
    83821 gggttatgag ccagttcgtt aagcagctca ctatccagcg tttcgccatc cattaaagcg
    83881 gaaagttgtt ctttctgcat gcctaatacc cttatccagt atcccgctat cgtcaacgcc
    83941 tgataagcgg ttgaactttg ttatcaatag cttccctcgc tcggaagata cgtgaacgca
    84001 ccgtacctac cggacaatcc atgatagcgg ctatctcttc atagctcagg ccatccagct
    84061 cccgcaaggt tattgccatg cgtaaatctt ccgggaggga ctcaatagtt cggaaaacta
    84121 tctgtctcag ttcttctgac aacattaagt tctcagggtt cgaaatttct ttcaacgcgc
    84181 cgccactttc gaagttttca gcttcaatgg catccacatc actggaaggt ggacgacgcc
    84241 cctgagcaac caggtaattt ttcgctgtat ttacagcaat ccgatacagc catgtataaa
    84301 aagcgctatc tccccggaac gaatccagcg cacgataggc tttaataaaa gcttcttgta
    84361 ccacatcggg aacatcaccc gacggcacat agcgggaaac cagactcgcc actttatgct
    84421 gatagcgcac taccagtaag ttaaaggctt tctgatctcc cttctggacc cgttcaacca
    84481 ggacctggtc cgttaactgc tcgctcatcc gaggtaaagt ctccccaaac caaatttcca
    84541 cgcgctatcg aaacgccact ccattagctg caatttgagc aagcaaaggg ttagagtgtc
    84601 tcgtttttgt aaagttccgt aacgcatctg tttttgtttg tcatgctgta gacggatcat
    84661 tatctatcat tataagtcta cagaatctga acatcgcatt atctgtgtag aaatgcccat
    84721 ttaactgcct gaagagtaac ccaacggcct ttttatttca ccacctaatc ctccaccagc
    84781 cagtaacttc tctttttctc gccgccctgc gtcagcgtgt ttagtcactg taacaaatat
    84841 taaaatagca ggtgtttatt cgcacaacat gatgctatgc tgaccaaacc gtgtttagta
    84901 aattaaacaa agaaaatgaa tactctcctt gaacattcat gtgacgtgtt gattatcggt
    84961 agcggcgcag ccgggctttc actggcgcta cgcctggctg accagcatca ggtcatcgtt
    85021 ctaagtaaag gcccggtaac ggaaggttca acattttatg cccagggcgg tattgccgcc
    85081 gtgtttgatg aaactgacag cattgactcg catgtggaag acacattgat tgccggggct
    85141 ggtatttgcg atcgccatgc agttgaattt gtcgccagca atgcacgatc ctgtgtgcaa
    85201 tggctaatcg accagggggt gttatttgat acccacattc agccgaatgg cgaagaaagc
    85261 taccatctga cccgtgaagg tggacatagt caccgtcgta ttcttcatgc cgccgacgca
    85321 actggtagag aagtagaaac cacgctggtg agcaaggcgc tgaaccatcc gaatattcgc
    85381 gtgctggagc gcagcaacgc ggttgatctg attatttctg acaaaattgg cctgccgggc
    85441 acgcgacggg ttgttggcgc gtgggtatgg aaccgaaata aagaaaaggt ggaaacctgc
    85501 cacgcaaaag cggtggtgct ggcaaccggc ggtgcgtcga aggtttatca gtacaccacc
    85561 aatccggata tttcttctgg cgatggcatt gctatggcgt ggcgcgcagg ctgccgggtt
    85621 gccaatctcg aatttaatca gttccaccct accgcgctgt atcacccaca ggcacgcaat
    85681 ttcctgttaa cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg
    85741 cgttttatgc ccgattttga tgtgcgcggc gaactggccc cgcgcgatat tgtcgcccgc
    85801 gccattgacc atgaaatgaa acgcctcggc gcagattgta tgttccttga tatcagccat
    85861 aagcccgccg attttattcg ccagcatttt ccgatgattt atgaaaagct gctcgggctg
    85921 gggattgatc tcacacaaga accggtaccg attgtgcctg ctgcacatta tacctgcggt
    85981 ggtgtaatgg ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag
    86041 gtgagttata ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt
    86101 ctggtctatg gctggtcggc ggcggaagat atcaccagac gtatgcctta tgcccacgac
    86161 atcagtacgt taccgccgtg ggatgaaagc cgcgttgaga accctgacga acgggtaata
    86221 attcagcata actggcacga gctacgtctg tttatgtggg attacgttgg cattgtgcgc
    86281 acaacgaagc gcctggaacg cgccctgcgg cggataacca tgctccaaca agaaatagac
    86341 gaatattacg cccatttccg cgtctcaaat aatttgctgg agctgcgtaa tctggtacag
    86401 gttgccgagt tgattgttcg ctgtgcaatg atgcgtaaag agagtcgggg gttgcatttc
    86461 acgctggatt atccggaact gctcacccat tccggtccgt cgatcctttc tcccggcaat
    86521 cattacataa acagataaaa agcctgggtt agcgccgtat acgcttcgga atagttctgg
    86581 tctggcccac gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc
    86641 agcagcaccc gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa
    86701 tgccagccca tacttaatgc cagctccgta aaactattac caatctgctc tggcagcacc
    86761 acgcagaaaa atccctcttc ggtaatgcac tccgccgcgc aagtaagtaa agtctggtga
    86821 tcaagtgagg tggtgtagcg cgcctgttcc cgctgcggcg tcgcgcactc cactccttgc
    86881 tcataatacg gcgggttgct gatgattaaa tcaaagcgcg cggtctgctg tgttacccac
    86941 tgctgaatat ccgccgtatg gacgttaatt ctctctaacc acggggactg ggcgacattt
    87001 tcctgcgcct gagtggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca
    87061 ctggttcgct gcgccagcat cagtgccagc aagccactac ccgcaccgat atcaaggcaa
    87121 cgttttaccc cagccaccgg tgcccatgcg cccagcaata tgccatccgt tcccactttc
    87181 atcgcacagc gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc
    87241 acggatgtag actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg
    87301 tgagaacaat acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt
    87361 ctataatcag cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga
    87421 actcgacgaa agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat
    87481 tcaggctgcc gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac
    87541 aggcaccggt aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc
    87601 gcgtaagaaa tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat
    87661 gcaggtggcc gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat
    87721 caccggcggc gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt
    87781 ggtcgccacg accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc
    87841 ggttgaaacg ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga
    87901 tatcgaacat attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac
    87961 gctggaaggc gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt
    88021 ttctgccaat ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga
    88081 tgacctggaa cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg
    88141 ctcaattgtg tttgtgcgta agcgtgagcg tgtgcatgag ctggcaaact ggctgcgcga
    88201 agcgggcatc aacaactgct atctcgaagg tgagatggta cagggcaagc gtaacgaagc
    88261 gatcaaacgt ttgaccgaag gtcgcgtaaa cgtactggtt gcaaccgatg ttgccgcgcg
    88321 cggtatcgac attcctgacg tcagccacgt ctttaacttc gatatgccgc gcagtggcga
    88381 tacttatttg caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcaatttc
    88441 gctggtagaa gcccatgacc atctgctgct gggcaaagtt ggtcgctata ttgaagagcc
    88501 aattaaagct cgcgtaattg atgagttacg cccgaaaacg cgtgcaccaa gcgagaagca
    88561 gaccggcaag ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagcaaaaga
    88621 gaaagagaag ccgcgggtga aaaaacgcca tcgcgatacc aaaaatattg gtaagcgccg
    88681 taaaccaagc ggaacgggcg tgccaccgca aacgacagaa gagtaatctc aatgccgggt
    88741 ttaagcccgg cattattttt atcccggcaa aaaacaatct tcaacacact gaataaaagt
    88801 gtgcatcgcc gggctaaccg cttttccggc atggtgagcg cacattgccg taatggtctg
    88861 cgagtgttcg ccaaaaggca attcaattaa ttctccgctc tcaagctctt tttccaccgc
    88921 aaagcggggc aagtaactga caccgatatt cgccgcgaca caacgtttga tactttcaat
    88981 gcttaacagc tcaatggtgt tttccaccgt gatccgccgc tggcgcagcg tgctctcaaa
    89041 tatctgccgg aatacacatt gcggttcgtt gataataaag ctacaggcgt tatgtcttcc
    89101 cggctcagta aaatcgacat ctgcaatttg cggtgaagct accagcacca gcgattgctc
    89161 acccaattct cgtcggttca gggcatcatc attccctacg cgataaaaaa ccccgacatc
    89221 ggcctcatca ttcaatagcg catcacggat cacgtaacag ttcagagact gcaacgataa
    89281 acgcactttt ggggcgcgtt gccgaaaacg ctgcaacacc tgcggcattc ggtaagagag
    89341 caacgtttcg cccgaaacaa cgcgtagttc cccgtccgga tccgactcct ttttggcagc
    89401 ctcacgaagg gtatccatca ctctggtgag ttcataaatg tgcggcagca gctttttacc
    89461 ttcgcgagtg aggcacatcc gtcggccaat tttctcaaat aactgtaccg agaactcctg
    89521 ctcaagctgc tgaatatgaa aagtcaccgt cgattgtgta cagcacaatt tttgcgaagc
    89581 tcgcaaaaag gaaccctctt ccaccacggt tttaagcgta ataaaacggc gcagatccat
    89641 aaccccaaac ctatcgaaaa tatcgaattt agaatgtaaa aacattcatt tttttaaata
    89701 ttctgtgtcg ggtactgtct accaaaacag aggagataac aagtgacacc gaccctttta
    89761 agtgcttttt ggacttacac cctgattacc gccatgacgc caggaccgaa caatattctc
    89821 gcccttagct cagccacaac gcacggattt catcagagta cccgcgtgct ggcagggatg
    89881 agtctgggat ttttaatcgt catgttgctg tgtgcgggca tttcgttttc actggctgtt
    89941 atcgacccgg cagctgtgca tctgttgagt tgggcggggg cggcatatat tgtctggctg
    90001 gcgtggaaaa tcgctaccag tccaacaaag gaagacgggc ttcagacaaa accaatcagt
    90061 ttttgggcca gctttgcttt gcagtttgtg aacgtcaaaa tcattttgta cggtgttacg
    90121 gcactgtcga catttgttct gccgcaaaca caggcgttaa gctggatagt tggcgtcagc
    90181 gttttgctgg caatgattgg gacgtttggt aatgtgtgct gggcgctggc ggggcatctg
    90241 tttcagcgat tgtttcgcca gtatggtcgc cagttaaata tcgtgctggc gctgctgctg
    90301 atctattgcg cggtacgcat tttctattaa tgaaaaaaag cggaagaggt cgccctcttc
    90361 cgcttagtaa catgctactt aagccttaca ggctttcagt aaaggtacga gcgataacgt
    90421 cgcgctgctg ttccggagtc agagagttaa agcgaactgc ataaccggat acacggatgg
    90481 tcagctgcgg atatttttcc ggatgcttaa ctgcatcttc cagagtttca cgacgcagaa
    90541 cgttaacgtt caggtgctga ccaccttcaa cgcgaacttc tggtttcact tctactggaa
    90601 cttcacggta ctcaatgtca cccagtttgc ttactgcaac cacttcgtct tctgcataac
    90661 ctgctttcgc aacgatgcaa cgcgcttcgc ctttttcgct gtccagcagc cagaaagagt
    90721 tcagcagatc gtcgttagcg gctttagtaa tctggatacc tgtaatcatg tgatgcctcc
    90781 ccggcaaaat tatttgattt gttcagcctg tcgcggccaa ttggtaaaac cattgttgct
    90841 tgagtgtata tatactcctc aaacaccctt gaatctttga tttaaatcaa taaaaaccac
    90901 acatcaagtg tggtcgcaaa tagattttat tgttttacat caacttatgc gggtgtgaaa
    90961 ttttaccaat ttacattttt ttgcactcgt ttaagtctaa aaaatgagca tgattttgtt
    91021 ctgtagaaag aagcagttaa gctaggcgga ttgaagattc gcaggagagc gagatggcta
    91081 acgaattaac ctggcatgac gtgctggctg aagagaagca gcaaccctat tttcttaata
    91141 cccttcagac cgtcgccagc gagcggcagt ccggcgtcac tatctatcca ccacaaaaag
    91201 atgtctttaa cgcgttccgc tttaccgagt tgggtgacgt taaagtggtg attctcggcc
    91261 aggatcctta tcacggaccg ggacaggcgc atgggctggc attttccgtt cgccccggaa
    91321 tcgccactcc tccgtcactg ctgaatatgt ataaagagct ggaaaatact attccgggct
    91381 tcacccgccc taatcatggt tatcttgaaa gctgggcgcg tcagggcgtt ctgctactca
    91441 atactgtgtt gacggtacgc gcaggtcagg cgcattccca cgccagcctc ggctgggaaa
    91501 ccttcaccga taaagtgatc agcctgatta accagcatcg cgaaggcgtg gtgtttttgt
    91561 tgtggggatc gcatgcgcaa aagaaagggg cgattataga taagcaacgc catcatgtac
    91621 tgaaagcacc gcatccgtcg ccgctttcgg ctcatcgtgg attctttggc tgcaaccatt
    91681 ttgtgctggc aaatcagtgg ctggaacaac atggcgagac gccgattgac tggatgccag
    91741 tattaccggc agagagtgag taaatttgcg ggaaaatgcc ggatggcaga gttgccaccc
    91801 ggctgattta tcaggcttta ttctgacgcc accattcgcc aagaagaacg ccggttgcga
    91861 cagagatatt cagcccggca acgttgccag taccatcaat cttcacgcgc agatcgttcg
    91921 gatcgcgtgc ggcatccggt aacccttcat attcctgacc cagcaccagt accattttcg
    91981 ctggcagact ggttttgaac agcggtttac cctgctcgct ggaagttgtc actacggtgt
    92041 aacccgcctg acggaaatca tccagcacgt taacaatgtt gtcgccagta atcggctgaa
    92101 cgtgctctgc gccgccttct gcggtacgga tagccgcccc cgactccagc agtgccgcat
    92161 cctgcaccac aacacctttc acgccaaagt gcgcgcagct gcgcatcatg ccacccaggt
    92221 tatgcgggtt agattcgttt tccagtgcca gaacacaatc ctgcgcgcct gcctggctta
    92281 cccactgctg cacggttgtg ccattacgct ttttgatcag gaagcaaacg ccaccgtgat
    92341 gttccgtgcc tgacgctttt gtcaattccg cttcatctac cacatggtac gctttgcggt
    92401 ttgctgccat ccagcgcaag gcttctttaa aacgcggcgt tacactctgg ataaaccagg
    92461 cgcgaacaat cgcttccgga cggctctgga acagtgcctg acaggcgttt tcgccgtaga
    92521 cgcgggtttc ttccgcacgc tgacgacgca acacttccgg atcaataaaa cttttaccac
    92581 tgatgccacc gtgatcggcc ttttccggcg tctcatcacc cggcgcgcgg gaaaccgtgc
    92641 gccacggcga atcttcccac ttgcggtcac ggggctgact ctgtttgtca tcgcgggcgg
    92701 ggcgacggcc accgtcagca cgagattttc ctggacgccc gccccctttc ccggtacgcg
    92761 ggttgtgggt acgtttatca gaatcatcat cactgcggac atacatcact ttgaccttgc
    92821 cgcttttacc tttcatttca tcgttcatgc ttttctccac cagcgctgcg cgaagcgcgc
    92881 agattacccg aagtccgcgc ggttcgccat gatttcgtac caaagcctgc gactatcata
    92941 cctattgaat aaaacagatt gttgtctgga acaatgtccc cgataatatg taacatatta
    93001 gaaacatacc ggcgttgttg ccgataagtc tccttactca tcccgaggtt agttatgaat
    93061 accgtttgta cccattgtca ggccatcaat cgcattcccg acgatcggat cgaagatgcg
    93121 gcaaaatgcg gacgctgcgg tcacgacttg tttgacggag aggtgattaa tgcgaccggt
    93181 gaaacgctcg acaaattgct gaaagatgat ctaccagtgg tgatcgactt ctgggcaccg
    93241 tggtgcggcc cctgccgtaa tttcgcacca atttttgaag atgtcgcgca agagcgtagc
    93301 ggtaaagtgc gctttgtgaa agtgaatacc gaagctgaac gtgaattgag cagtcgcttt
    93361 ggaattcgta gtataccgac gatcatgatt ttcaaaaacg gtcaggttgt cgacatgctt
    93421 aatggcgcag taccgaaagc gccgttcgat agctggctga acgaatctct ttaatcttac
    93481 cggggcgcat cttgtgcccc gttttctcct ctgcgacaat ggcgtttttt cgacgctctc
    93541 ttatgaccga aaacgctgtt ctccagttac gcgccgagcg tattgcgcgc gcaacacgtc
    93601 cttttcttgc ccgcggtaat cgcgttcgtc gctgccaacg ctgtcttttg ccagagaaat
    93661 tatgtctctg ttcgacaatt accccagcac aagcaaaaag tcgcttctgt ttgctgatgt
    93721 tcgacaccga gccaatgaag cccagtaata ccgggcgtct gattgctgat attttgcctg
    93781 ataccgttgc gtttcaatgg tcgcgtaccg aaccctcgca ggatttgctg gatctggtac
    93841 aaaacccgga ttatcagcca atggtggtct ttcccgcctc gtatgctgat gagcaacggg
    93901 aagtgatctt cacaccacct gccggtaagc caccgctgtt tatcatgctc gatggtacct
    93961 ggccggaagc tcgcaagatg tttcgtaaaa gtccgtatct ggataatctt cccgtcattt
    94021 ccgtcgatct ttcccggctt tctgcctatc gcctgcgtga agcccaggct gaaggccaat
    94081 attgtactgc cgaggtagcc atcgcactgt tagatatggc cggcgatacc ggggcggcag
    94141 cagggttagg cgagcacttc acccgcttta aaacgcgcta tctggcagga aaaacgcaac
    94201 atctgggtag catcacagca gaacagttag aaagcgttta aaatcattcg gtcacttctg
    94261 cgggagaccg gtatgagtca gcgaggactg gaagcactac tgcgaccaaa atcgatagcg
    94321 gtaattggcg cgtcgatgaa acccaatcgc gcaggttacc tgatgatgcg taacctgctg
    94381 gcgggaggct ttaacggacc ggtactcccg gtgacgccag cctggaaagc ggtgttgggt
    94441 gtgttggcct ggccggatat tgccagcttg ccctttacac ccgaccttgc ggttttatgt
    94501 accaatgcca gccgtaatct tgctcttctg gaagagctcg gcgagaaagg ctgtaaaacc
    94561 tgcattattc tttccgcccc ggcatcgcaa cacgaagatc tccgcgcctg cgccctgcgc
    94621 cataacatgc gcctgcttgg accaaacagt ctgggtttac tggctccctg gcaaggtctg
    94681 aatgccagct tttcgcctgt gccgattaaa cgcggcaagc tggcgtttat ttcacagtcg
    94741 gctgccgtct ccaacaccat cctcgactgg gcgcaacagc gtgagatggg cttttcctac
    94801 tttattgcgc tcggcgacag cctagatatc gacgttgatg aattgcttga ctatctggca
    94861 cgcgacagta aaaccagcgc catcctgctc tatctcgaac agttaagcga cgcgcgacgc
    94921 tttgtttcgg cggcccgtag tgcctcgcgt aataaaccga ttctggtgat taaaagcgga
    94981 cgtagcccgg cagcacagcg actgctcaac acgacggcag gaatggatcc ggcgtgggat
    95041 gcggcgattc agcgtgccgg tttgttgcgg gtgcaggata cccacgagct gttttcggcg
    95101 gtggaaaccc tcagccatat gcgcccgctg cgtggcgacc ggttgatgat tatcagcaac
    95161 ggtgctgcgc ctgccgcgct ggcgctggat gccttatggt cacgcaatgg caagctggca
    95221 acgctaagcg aagaaacctg ccagaaactg cgcgatgcac tgccaggaca tgtggcaatc
    95281 tctaacccgc tcgatctgcg cgatgacgcc agcagcgagc attatgtcaa aacgctggac
    95341 attctgctcc acagccagga ttttgatgcg ctgatggtta ttcattcgcc cagcgccgct
    95401 gctcccgcaa cagaaatcgc gcaagtatta attgaagcgg taaagcatca tccccgcagc
    95461 aagtatgttt ctctgctgac gaactggtgc ggcgagcact cctcgcaaga ggcacgacgt
    95521 ttattcagcg aagccgggct gccgacctac cgtaccccgg aaggaaccat cactgctttt
    95581 atgcatatgg tggagtaccg gcgtaatcag aagcaactac gcgaaacgcc ggcgttgccc
    95641 agcaatctga cttccaatac cgcaaaagcg catcttctgt tgcaacaggc gattgccgaa
    95701 ggggctacgt cgctcgatac ccatgaagtt cagcccatcc tgcaatcgta tgggatgaac
    95761 acgctcccta cctggattgc cagcgatagc accgaagcgg tgcatattgc cgaacagatt
    95821 ggttatccgg tggcgctgaa attgcgttcg ccggatattc cacataaatc ggaagttcag
    95881 ggcgtcatgc tgtacctgcg tacagccaat gaagtccagc aagcggcaaa cgctattttc
    95941 gatcgcgtaa aaatgacctg gccgcaggcg cgggtccacg gcctgttggt gcaaagtatg
    96001 gctaaccgtg ctggcgctca ggagttgcgg gttgtggttg agcacgatcc ggtttttggg
    96061 ccgttgatca tgctgggtga aggcggtgtg gagtggcgtc ctgaagatca agccgtcgtc
    96121 gcactgccgc cgctgaatat gaacctggcc cgctatctgg ttattcaggg gatcaaaagt
    96181 aaaaagattc gtgcgcgcag tgcgctacgc ccattggatg ttgcaggctt gagccagctt
    96241 ctggtgcagg tttccaactt gattgtcgat tgcccggaaa ttcagcgtct ggatattcat
    96301 cctttgctgg cttctggcag tgaatttacc gcgctggatg tcactctgga tatcgcgccg
    96361 tttgaaggcg ataacgagag tcggctggca gtgcgccctt atccgcatca gctggaagaa
    96421 tgggtagaat tgaaaaacgg tgaacgctgc ttgttccgcc cgattttgcc agaagatgag
    96481 ccacaacttc agcaattcat ttcgcgggtc accaaagaag atctttatta ccgctacttt
    96541 agcgagatca acgaatttac ccatgaagat ttagccaaca tgacgcagat cgactacgat
    96601 cgggaaatgg cgtttgtagc ggtacgacga attgatcaaa cggaagagat cctcggcgtc
    96661 acgcgtgcga tctccgaccc tgataacatc gatgccgaat ttgccgtgct ggttcgctcg
    96721 gatctcaaag ggttaggctt gggtcgacgc ttaatggaaa agttgattac ctatacgcga
    96781 gatcacggac tacaacgtct gaatggtatt acgatgccaa acaatcgtgg catggtggcg
    96841 ctggcccgca agctcgggtt taacgttgat atccagctcg aagaggggat cgttgggctt
    96901 acgctaaatc ttgcccaacg cgaggaatca tgagtaaggt actggaaatg ttgaccactt
    96961 aatcgggact ggtgttatta ttgcacgctt atgtcgtctg cattgcacag aggacccttc
    97021 aatgaacaga gaagaaatgc actgtgatgt tgtcaaaatt taagcgtaat aaacatcaac
    97081 aacaccttgc ccaactaccc aagatttctc aatcagttga tgatgtcgat ttcttttacg
    97141 ctcccgccga cttccgggag acgctgctgg aaaaaatagc cagcgcgaag cagcgcattt
    97201 gcattgtcgc cctgtatctc gaacaggatg acggtggcaa aggcattctg aacgcgttgt
    97261 atgaagctaa aaggcagcgt ccggaactgg atgtgcgggt gctggtcgac tggcatcgtg
    97321 cacaacgtgg acgcattggc gctgcggcat ctaacactaa cgctgactgg tactgtcgca
    97381 tggcgcagga aaatccgggc gtagatgttc cggtttatgg cgttccaatc aatacccgtg
    97441 aagcccttgg tgttctgcac tttaaaggct ttatcatcga cgatagcgta ctttatagcg
    97501 gtgccagcct gaacgatgtt tacctgcatc agcacgataa atatcgctac gaccgttatc
    97561 atcttatccg taaccgtaag atgtcagaca ttatgtttga atgggttaca cagaatatta
    97621 tgaatggccg cggcgttaat cgtctggatt atgttaatcg gccaaaaagc ccggaaatca
    97681 agaacgatat tcgtctgttc cgccaggagc tgcgtgatgc cgcttatcat ttccagggcg
    97741 atgccgacaa cgatcagctt tccgtaacgc cgctagtggg gctggggaaa tcgagtctgt
    97801 tgaacaagac cattttccat cttatgcctt gtgccgagca gaaactaacc atctgtacgc
    97861 catacttcaa cctgccagca atccttgtgc gcaatattat ccagttgctg cgcgaaggga
    97921 aaaaggtcga aattattgtt ggtgataaaa ccgcgaacga cttctacatt ccggaagatg
    97981 aacctttcaa gataattggc gcattgcctt atctctatga gatcaatctg cgtcgtttcc
    98041 tgagccgttt gcagtattac gtcaatactg accagctagt ggttcggtta tggaaagatg
    98101 acgacaacac ctatcaccta aaagggatgt gggttgatga taagtggatg ttgatcaccg
    98161 gtaataacct gaacccgcgc gcctggcgtc tggatctgga aaacgccatt ttgatccacg
    98221 atccacaact ggagcttgcg ccgcagcgag agaaagaact ggagctgatc cgcgagcata
    98281 ccaccatcgt taagcactat cgcgatctgc aaagtattgc cgattatccg gtgaaggttc
    98341 gtaaactcat ccgccgtttg cgccgtatcc gcatcgaccg attaattagc cgcatcctgt
    98401 aatcacaacc ccgtcctgta cggggtttgt tttttggagg ccacgttttg cgtattcttt
    98461 ttgtctgttc gctattgtta ctttctggat gcagccatat ggctaacgat agctggagcg
    98521 ggcaggataa agctcaacac tttatcgcct cggcgatgct ttccgccgcc ggaaatgaat
    98581 attcacagca tcaggggatg agccgggatc gcagtgccat gtttggtttg atgttctctg
    98641 tcagtttggg ggcgtcaaaa gagttttggg atagccgccc cgaagggagc ggctggagct
    98701 ggaaggattt ggcctgggat gtcgccggtg caagcaccgg ctataccgtc tggcaactga
    98761 cccgtcacta aagacgcatc cccttccctt tgcgatgtag catcaaagaa accagaaacg
    98821 ccaccacggc catcaaggtc acataccaga agaaggctgt ttccattcct attgatttca
    98881 gcgacaacgc tacgtactcc gccgaaccac caaatatagc attagcgacc gcatatgaca
    98941 gaccaacgcc taatgcgcga acctgtgccg ggaacatctc agccttcagt attccactga
    99001 ttgatgtata aaaactcact atcagcaggg cacacatcac cagaccaaaa gcggcataag
    99061 gcgaggaaac gttttgcaat gctgagagaa taggaacggt aaaaatggct gccagcgaac
    99121 cgaaacataa cattgaggta cggcgaccaa tcttatccga cagcgcgcca atgagtggtt
    99181 gaataagcat gaatacaaac aatgcggcag tcataatgcc actcgccacg ttggcatgca
    99241 ttcccgcagt atttaccaga tacttctgca tataagtggt gaaggtatag aaacaaaggg
    99301 agcccgcagc ggtaaaaccg agaaccatga tgaatgcacg gcgattgcgc cataatcctt
    99361 tcagagatcc agcttctttt aaagcgcgcg tttcttgttg cgaagtttca tctaactgac
    99421 gacgtaacca caacgccaca acagctaaca cagctcctaa cgcgaaagga atacgccatc
    99481 cccactctct gagtgcagag tcttccatgg tgtgttgtaa aaccacgaca accagtaggg
    99541 ctagcagttg tccgccgatc aacgtcacat actgaaatga tgcgtaaaaa cctttgcgcc
    99601 cttcaacggc aacttcactc atataggtgg cgctggtgcc atattctccg ccaacggata
    99661 atccctgaaa taaacgcgcg agaagcaata atgccggagc ccacgtaccg attgtttcat
    99721 aaccaggcaa acaggcgatt accagtgagc cgaaacacat catacacacc gatagcagca
    99781 tcgatttttt gcgaccatgt ttatcggcta tacggccaaa tagccaaccg cctattgggc
    99841 gcatcaggaa tcccgcagca aaaacacctg ctgtttgtag tagttgagtc gtcgtgttcc
    99901 cggaagggaa gaagatgtgg gcaaagtaga gtgaacagaa cgagtagaca tagaaatcga
    99961 accactcgac cagattacct gaagaggccc ccacaatcgc ccaaatgcgg cgacgagtat
   100021 cactacttgt cagtttgctg tctgccgtta cagtactttc agccatgcca ttatgtctcc
   100081 tgccgtaatc cgatgctttt gtcggtcgct tttgtttatt tcttttggca aagaaaatgt
   100141 tatacatttg ttacgtatca ttatgcaacc ttaaccatga atttagttag caggaaaatg
   100201 gttattgagg agcttaagga taaatttctg gtaaggagga cacgtatgga agtaggcaag
   100261 ttggggaaac cgtatccgtt gctgaatctg gcatatgtgg gagtataaga cgcgcagcgt
   100321 cgcatcaggc atttttttca ccaacgcaaa aaggccatcc tgacggatgg ccttt
//
LOCUS       DAZFTN010000004        99615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFTN010000004 DAZFTN010000000
VERSION     DAZFTN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 99615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..1341)
                     /locus_tag="ACHKV2_000315"
     CDS             complement(76..1341)
                     /locus_tag="ACHKV2_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6938278.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHKV2_000316"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHKV2_000316"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHKV2_000317"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHKV2_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO6938279.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ACHKV2_000318"
     CDS             2580..2663
                     /locus_tag="ACHKV2_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938280.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ACHKV2_000319"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ACHKV2_000319"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO6938281.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHKV2_000320"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHKV2_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO6938282.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHKV2_000321"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHKV2_000321"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO6938283.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHKV2_000322"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHKV2_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO6938284.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHKV2_000323"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHKV2_000323"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO6938285.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ACHKV2_000324"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ACHKV2_000324"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO6938286.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ACHKV2_000325"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ACHKV2_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO6938287.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ACHKV2_000326"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ACHKV2_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO6938288.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ACHKV2_000327"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ACHKV2_000327"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO6938289.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ACHKV2_000328"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ACHKV2_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO6938290.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHKV2_000329"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHKV2_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO6938291.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHKV2_000330"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHKV2_000330"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO6938292.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHKV2_000331"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHKV2_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO6938293.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHKV2_000332"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHKV2_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO6938294.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHKV2_000333"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHKV2_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO6938295.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="ACHKV2_000334"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="ACHKV2_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO6938296.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHKV2_000335"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHKV2_000335"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO6938297.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHKV2_000336"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHKV2_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO6938298.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHKV2_000337"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHKV2_000337"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO6938299.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHKV2_000338"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHKV2_000338"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO6938300.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHKV2_000339"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHKV2_000339"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO6938301.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHKV2_000340"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHKV2_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO6938302.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHKV2_000341"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHKV2_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO6938303.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24228..25382)
                     /gene="metK"
                     /locus_tag="ACHKV2_000342"
     CDS             complement(24228..25382)
                     /gene="metK"
                     /locus_tag="ACHKV2_000342"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO6938304.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25438..25689
                     /gene="yqgD"
                     /locus_tag="ACHKV2_000343"
     CDS             25438..25689
                     /gene="yqgD"
                     /locus_tag="ACHKV2_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO6938305.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25800..25943
                     /gene="yqgG"
                     /locus_tag="ACHKV2_000344"
     CDS             25800..25943
                     /gene="yqgG"
                     /locus_tag="ACHKV2_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO6938306.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26037..26168
                     /gene="yqgB"
                     /locus_tag="ACHKV2_000345"
     CDS             26037..26168
                     /gene="yqgB"
                     /locus_tag="ACHKV2_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO6938307.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26177..28153
                     /gene="speA"
                     /locus_tag="ACHKV2_000346"
     CDS             26177..28153
                     /gene="speA"
                     /locus_tag="ACHKV2_000346"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO6938308.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            28299..29030
                     /locus_tag="ACHKV2_000347"
     CDS             28299..29030
                     /locus_tag="ACHKV2_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6938309.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            29166..30086
                     /gene="speB"
                     /locus_tag="ACHKV2_000348"
     CDS             29166..30086
                     /gene="speB"
                     /locus_tag="ACHKV2_000348"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO6938310.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(30292..31050)
                     /gene="loiP"
                     /locus_tag="ACHKV2_000349"
                     /gene_synonym="yggG"
     CDS             complement(30292..31050)
                     /gene="loiP"
                     /locus_tag="ACHKV2_000349"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO6938311.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            31328..33319
                     /gene="tkt"
                     /locus_tag="ACHKV2_000350"
     CDS             31328..33319
                     /gene="tkt"
                     /locus_tag="ACHKV2_000350"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6938312.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRTASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            33633..34076
                     /gene="cmtB"
                     /locus_tag="ACHKV2_000351"
     CDS             33633..34076
                     /gene="cmtB"
                     /locus_tag="ACHKV2_000351"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO6938313.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            34104..35492
                     /gene="cmtA"
                     /locus_tag="ACHKV2_000352"
     CDS             34104..35492
                     /gene="cmtA"
                     /locus_tag="ACHKV2_000352"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO6938314.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            35507..36784
                     /gene="yggP"
                     /locus_tag="ACHKV2_000353"
     CDS             35507..36784
                     /gene="yggP"
                     /locus_tag="ACHKV2_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6938315.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            36781..37746
                     /gene="glpX"
                     /locus_tag="ACHKV2_000354"
     CDS             36781..37746
                     /gene="glpX"
                     /locus_tag="ACHKV2_000354"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6938316.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            37768..38277
                     /gene="fumE"
                     /locus_tag="ACHKV2_000355"
     CDS             37768..38277
                     /gene="fumE"
                     /locus_tag="ACHKV2_000355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO6938317.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            38274..38987
                     /locus_tag="ACHKV2_000356"
     CDS             38274..38987
                     /locus_tag="ACHKV2_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO6938318.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(38959..39636)
                     /locus_tag="ACHKV2_000357"
     CDS             complement(38959..39636)
                     /locus_tag="ACHKV2_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6938319.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(39630..40307)
                     /locus_tag="ACHKV2_000358"
     CDS             complement(39630..40307)
                     /locus_tag="ACHKV2_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6938320.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(40295..41002)
                     /locus_tag="ACHKV2_000359"
     CDS             complement(40295..41002)
                     /locus_tag="ACHKV2_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO6938321.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(41003..41581)
                     /locus_tag="ACHKV2_000360"
     CDS             complement(41003..41581)
                     /locus_tag="ACHKV2_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO6938322.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(41604..42035)
                     /locus_tag="ACHKV2_000361"
     CDS             complement(41604..42035)
                     /locus_tag="ACHKV2_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO6938323.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            42407..43426
                     /gene="epd"
                     /locus_tag="ACHKV2_000362"
     CDS             42407..43426
                     /gene="epd"
                     /locus_tag="ACHKV2_000362"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO6938324.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            43476..44639
                     /gene="pgk"
                     /locus_tag="ACHKV2_000363"
     CDS             43476..44639
                     /gene="pgk"
                     /locus_tag="ACHKV2_000363"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO6938325.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            44854..45933
                     /gene="fbaA"
                     /locus_tag="ACHKV2_000364"
     CDS             44854..45933
                     /gene="fbaA"
                     /locus_tag="ACHKV2_000364"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6938326.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(45997..46095)
                     /locus_tag="ACHKV2_000365"
                     /pseudo
     CDS             complement(45997..46095)
                     /locus_tag="ACHKV2_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            46291..47151
                     /gene="mscS"
                     /locus_tag="ACHKV2_000366"
     CDS             46291..47151
                     /gene="mscS"
                     /locus_tag="ACHKV2_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6938327.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            47290..47925
                     /gene="argO"
                     /locus_tag="ACHKV2_000367"
     CDS             47290..47925
                     /gene="argO"
                     /locus_tag="ACHKV2_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO6938328.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            48018..48758
                     /gene="yggE"
                     /locus_tag="ACHKV2_000368"
     CDS             48018..48758
                     /gene="yggE"
                     /locus_tag="ACHKV2_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO6938329.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            48925..49821
                     /gene="ygfI"
                     /locus_tag="ACHKV2_000369"
                     /pseudo
     CDS             48925..49821
                     /gene="ygfI"
                     /locus_tag="ACHKV2_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(49818..51296)
                     /gene="scpC"
                     /locus_tag="ACHKV2_000370"
     CDS             complement(49818..51296)
                     /gene="scpC"
                     /locus_tag="ACHKV2_000370"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO6938330.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(51320..52105)
                     /gene="scpB"
                     /locus_tag="ACHKV2_000371"
     CDS             complement(51320..52105)
                     /gene="scpB"
                     /locus_tag="ACHKV2_000371"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO6938331.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(52116..53111)
                     /gene="meaB"
                     /locus_tag="ACHKV2_000372"
     CDS             complement(52116..53111)
                     /gene="meaB"
                     /locus_tag="ACHKV2_000372"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO6938332.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(53104..55248)
                     /gene="scpA"
                     /locus_tag="ACHKV2_000373"
     CDS             complement(53104..55248)
                     /gene="scpA"
                     /locus_tag="ACHKV2_000373"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO6938333.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(55452..56345)
                     /gene="argP"
                     /locus_tag="ACHKV2_000374"
     CDS             complement(55452..56345)
                     /gene="argP"
                     /locus_tag="ACHKV2_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO6938334.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            56728..57387
                     /gene="rpiA"
                     /locus_tag="ACHKV2_000375"
     CDS             56728..57387
                     /gene="rpiA"
                     /locus_tag="ACHKV2_000375"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO6938335.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            57642..58874
                     /gene="serA"
                     /locus_tag="ACHKV2_000376"
     CDS             57642..58874
                     /gene="serA"
                     /locus_tag="ACHKV2_000376"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6938336.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            59103..59162
                     /gene="ibsC"
                     /locus_tag="ACHKV2_000377"
     CDS             59103..59162
                     /gene="ibsC"
                     /locus_tag="ACHKV2_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO6938337.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            59496..59555
                     /locus_tag="ACHKV2_000378"
     CDS             59496..59555
                     /locus_tag="ACHKV2_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6938338.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(59656..60258)
                     /gene="fau"
                     /locus_tag="ACHKV2_000379"
     CDS             complement(59656..60258)
                     /gene="fau"
                     /locus_tag="ACHKV2_000379"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO6938339.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(60279..60462)
                     /gene="ssrS"
                     /locus_tag="ACHKV2_000380"
     ncRNA           complement(60279..60462)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKV2_000380"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(60504..60833)
                     /gene="zapA"
                     /locus_tag="ACHKV2_000381"
     CDS             complement(60504..60833)
                     /gene="zapA"
                     /locus_tag="ACHKV2_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6938340.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            61001..61579
                     /gene="ygfB"
                     /locus_tag="ACHKV2_000382"
     CDS             61001..61579
                     /gene="ygfB"
                     /locus_tag="ACHKV2_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO6938341.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            61605..62930
                     /gene="pepP"
                     /locus_tag="ACHKV2_000383"
     CDS             61605..62930
                     /gene="pepP"
                     /locus_tag="ACHKV2_000383"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO6938342.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            62927..64105
                     /gene="ubiH"
                     /locus_tag="ACHKV2_000384"
                     /gene_synonym="visB"
     CDS             62927..64105
                     /gene="ubiH"
                     /locus_tag="ACHKV2_000384"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO6938343.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            64129..65331
                     /gene="ubiI"
                     /locus_tag="ACHKV2_000385"
                     /gene_synonym="visC"
     CDS             64129..65331
                     /gene="ubiI"
                     /locus_tag="ACHKV2_000385"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO6938344.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(65405..65488)
                     /locus_tag="ACHKV2_000386"
     CDS             complement(65405..65488)
                     /locus_tag="ACHKV2_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938345.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            65779..66873
                     /gene="gcvT"
                     /locus_tag="ACHKV2_000387"
     CDS             65779..66873
                     /gene="gcvT"
                     /locus_tag="ACHKV2_000387"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO6938346.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            66897..67286
                     /gene="gcvH"
                     /locus_tag="ACHKV2_000388"
     CDS             66897..67286
                     /gene="gcvH"
                     /locus_tag="ACHKV2_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO6938347.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            67404..70277
                     /gene="gcvP"
                     /locus_tag="ACHKV2_000389"
     CDS             67404..70277
                     /gene="gcvP"
                     /locus_tag="ACHKV2_000389"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO6938348.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(70443..71876)
                     /gene="bglA"
                     /locus_tag="ACHKV2_000390"
     CDS             complement(70443..71876)
                     /gene="bglA"
                     /locus_tag="ACHKV2_000390"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO6938349.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFSEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            71921..72232
                     /gene="yqfB"
                     /locus_tag="ACHKV2_000391"
     CDS             71921..72232
                     /gene="yqfB"
                     /locus_tag="ACHKV2_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO6938350.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            72396..73055
                     /gene="yqfA"
                     /locus_tag="ACHKV2_000392"
     CDS             72396..73055
                     /gene="yqfA"
                     /locus_tag="ACHKV2_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO6938351.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(73132..74112)
                     /gene="ygfZ"
                     /locus_tag="ACHKV2_000393"
     CDS             complement(73132..74112)
                     /gene="ygfZ"
                     /locus_tag="ACHKV2_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO6938352.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            74355..74621
                     /gene="sdhE"
                     /locus_tag="ACHKV2_000394"
     CDS             74355..74621
                     /gene="sdhE"
                     /locus_tag="ACHKV2_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO6938353.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            74602..75009
                     /gene="ygfX"
                     /locus_tag="ACHKV2_000395"
     CDS             74602..75009
                     /gene="ygfX"
                     /locus_tag="ACHKV2_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO6938354.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(75049..75570)
                     /gene="fldB"
                     /locus_tag="ACHKV2_000396"
     CDS             complement(75049..75570)
                     /gene="fldB"
                     /locus_tag="ACHKV2_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO6938355.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            75682..76578
                     /gene="xerD"
                     /locus_tag="ACHKV2_000397"
     CDS             75682..76578
                     /gene="xerD"
                     /locus_tag="ACHKV2_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO6938356.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            76603..77313
                     /gene="dsbC"
                     /locus_tag="ACHKV2_000398"
     CDS             76603..77313
                     /gene="dsbC"
                     /locus_tag="ACHKV2_000398"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO6938357.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            77319..79052
                     /gene="recJ"
                     /locus_tag="ACHKV2_000399"
     CDS             77319..79052
                     /gene="recJ"
                     /locus_tag="ACHKV2_000399"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO6938358.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            79143..80241
                     /gene="prfB"
                     /locus_tag="ACHKV2_000400"
     CDS             join(79143..79217,79219..80241)
                     /gene="prfB"
                     /locus_tag="ACHKV2_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO6938359.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            80251..81768
                     /gene="lysS"
                     /locus_tag="ACHKV2_000401"
     CDS             80251..81768
                     /gene="lysS"
                     /locus_tag="ACHKV2_000401"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6938360.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(81811..82359)
                     /gene="idi"
                     /locus_tag="ACHKV2_000402"
     CDS             complement(81811..82359)
                     /gene="idi"
                     /locus_tag="ACHKV2_000402"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO6938361.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            82414..82485
                     /locus_tag="ACHKV2_000403"
     CDS             82414..82485
                     /locus_tag="ACHKV2_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938362.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(82482..82607)
                     /gene="yqfG"
                     /locus_tag="ACHKV2_000404"
     CDS             complement(82482..82607)
                     /gene="yqfG"
                     /locus_tag="ACHKV2_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO6938363.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(82609..84057)
                     /gene="uacT"
                     /locus_tag="ACHKV2_000405"
     CDS             complement(82609..84057)
                     /gene="uacT"
                     /locus_tag="ACHKV2_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO6938364.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            84493..86412
                     /gene="ygfT"
                     /locus_tag="ACHKV2_000406"
     CDS             84493..86412
                     /gene="ygfT"
                     /locus_tag="ACHKV2_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO6938365.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            86412..86900
                     /gene="ygfS"
                     /locus_tag="ACHKV2_000407"
                     /pseudo
     CDS             86412..86900
                     /gene="ygfS"
                     /locus_tag="ACHKV2_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(86936..88303)
                     /gene="ghxQ"
                     /locus_tag="ACHKV2_000408"
                     /gene_synonym="ygfQ"
     CDS             complement(86936..88303)
                     /gene="ghxQ"
                     /locus_tag="ACHKV2_000408"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO6938366.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(88339..89655)
                     /gene="guaD"
                     /locus_tag="ACHKV2_000409"
     CDS             complement(88339..89655)
                     /gene="guaD"
                     /locus_tag="ACHKV2_000409"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO6938367.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(89673..91073)
                     /gene="xanQ"
                     /locus_tag="ACHKV2_000410"
     CDS             complement(89673..91073)
                     /gene="xanQ"
                     /locus_tag="ACHKV2_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO6938368.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(91238..94108)
                     /gene="xdhD"
                     /locus_tag="ACHKV2_000411"
     CDS             complement(91238..94108)
                     /gene="xdhD"
                     /locus_tag="ACHKV2_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO6938369.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(94105..94884)
                     /gene="ygfM"
                     /locus_tag="ACHKV2_000412"
     CDS             complement(94105..94884)
                     /gene="ygfM"
                     /locus_tag="ACHKV2_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO6938370.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(94934..96262)
                     /gene="ssnA"
                     /locus_tag="ACHKV2_000413"
     CDS             complement(94934..96262)
                     /gene="ssnA"
                     /locus_tag="ACHKV2_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO6938371.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(96265..99363)
                     /gene="ygfK"
                     /locus_tag="ACHKV2_000414"
     CDS             complement(96265..99363)
                     /gene="ygfK"
                     /locus_tag="ACHKV2_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO6938372.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT        24148 a        24994 c        26393 g        24080 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatacccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacgt gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacccg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat
    23881 cgcttacact aatatttatc ttagttatga tttaagtgca tgaaaataat tgagttatat
    23941 catcatagat taaacgctgt tatctgcaat taagacttta ctgaaaagaa atgtaacaac
    24001 tgtgaaaacc gaataataat atggaaatat tacaaatctt tgtctgttgt ggttggtgta
    24061 atcggtttca gttgtgatga taatttgcgg tgaaacgtga tcaggaagaa tgatgttatt
    24121 gtgcaggcgg tttttttgcc ggatgcggcg tgaacgccta tccaccgcgg tgaggtaaaa
    24181 gaaaaaggcc agcgcgaggc tggcctttga aagcaggtga agaaagatta cttcagaccg
    24241 gcagcatcgc gcagcagctg cgctttgtcg gttttttccc acgggaaatg ttcacgacca
    24301 aagtgaccgt atgctgcggt ttctttgtag atcgggtgca gcagatccag catctgaatc
    24361 agaccgtatg ggcgcaggtc gaagaactca cgtaccagca gagtcagttg ttcagaaggc
    24421 actttctcag taccgaaagt ttctaccatg atggaagtcg gttcagccac gccgattgcg
    24481 taggaaacct gaatttcaca acgatcggcc aggccagcag caacgatgtt tttcgcgaca
    24541 taacgtgctg cgtaggctgc ggaacggtcc acttttgatg gatctttacc agagaatgca
    24601 ccgccaccgt gacgcgccat gccgccgtag gtatcaacga taattttacg accagtcaga
    24661 ccgcagtcac ccatcgggcc accgataaca aaacgaccgg tcgggttgat gaagaatttg
    24721 gtggcagaag tcagccattc agcgggcaga atcggcttga tgatctcttc cattaccgct
    24781 tcttgcagcg atttctggtc gatctcttca gagtgctgag tggaaagcac gacagcatcg
    24841 ataccaacga ttttgccgtc gtcatactgg aaagtcacct ggcttttcgc gtccgggcgc
    24901 agccacggca gagtgccgtt tttacgcact tcagcctgac gctgtaccag acggtgtgca
    24961 taggtgatag gtgctggcat cagcacgtcg gtttcattag ttgcgtagcc aaacatcaga
    25021 ccctggtcac ccgcgccctg ttccagcgga tcggcacggt caacgccctg gttgatgtca
    25081 ggagactgtt tgccgatagc gctcagaact gcacaggagt tagcgtcaaa gcccatgtcg
    25141 gaatgcacat agccaatttc gcgaacggtg ttacgggtga tctcttcgat gtctacccag
    25201 gcgctggtgg tgatttcgcc gccaactaaa accatgccgg tttttacgta ggtttcgcaa
    25261 gcaacgcgtg ctttcggatc ctgttcgagg atcgcgtcta aaacggcatc agagatttgg
    25321 tcagcaattt tgtcaggatg cccttcagag acggactcgg acgtaaaaag gtgttttgcc
    25381 atatttaata tcacctaaag agaatttggt tagctcaaac tgttgtgtgg attttctgtg
    25441 gtagcggatc ctaccacgac tctgcaggtt aaaaacactg gcagtctgag tgttaatcgg
    25501 tatggatgga ttaacatctg gatggctatt ttaggtcaat tcttcacccg atttccactt
    25561 ttttttgaat cgtgtctcat tctgttaaaa acgtggctgg aaatttttcc tgacaatgcc
    25621 ggcattctgc gtatttatct tttgcaattt tctgccattg tggggtataa aacgcggcgc
    25681 gcggcttaaa taaaaagcac acgacgtttc tttcgtgttg ccacttccag ccgggttgaa
    25741 atcagagttt tggcttgtgg gttcgtctta acaggcggcc gtggaggtga tacgaaataa
    25801 tgaaccgttg tctgctgctt aacctgtctc accgttctgg tgaagattcg ttccccgcac
    25861 tctgcatctc tgctttgcat acctgccgat gttataccca tctcggcgct tctcaggatt
    25921 caagagctgg ttacagttac tgaggactga acaagggcgc tcttgtaaaa acaagagttt
    25981 tctcgtggtt tcgccgaact gtcacactta cgttcggtta tgtgcttaat aatgttatga
    26041 aaaagaaacc ggttgcgcag ttggagcgtc agcattcact gctggaaaat ccatgtgctt
    26101 atgggttgtt atcgcagttc caggctgcga tagtcgttaa ctgttttaca cttaataaaa
    26161 taatttgagg ttcgctatgt ctgacgacat gtctatgggt ttgccttcgt cagcgggcga
    26221 acacggtgta ctacgctcca tgcaggaggt tgcaatgagc tcccaggaag ccagcaagat
    26281 gctgcgtact tacaatattg cctggtgggg caataactac tatgacgtta acgagctggg
    26341 ccacatcagc gtgtgcccgg acccggacgt cccggaagct cgcgtcgatc tcgcgcagtt
    26401 agtgaaaact cgtgaagcac agggtcagcg tctgcctgca ctgttctgtt tcccacagat
    26461 cctgcagcac cgtttgcgtt ccattaacgc cgcgttcaaa cgtgcgcggg aatcctacgg
    26521 ctataacggc gattacttcc ttgtttatcc gatcaaagtt aaccagcacc gtcgcgtgat
    26581 tgagtccctg attcattcgg gcgaaccgct gggtctggaa gccggttcca aagccgagtt
    26641 gatggcagtg ctggcacatg ctggcatgac ccgtagcgtc atcgtctgca atggttataa
    26701 agaccgcgaa tatatccgcc tggcattaat tggcgagaag atggggcaca aggtctatct
    26761 ggtcattgag aagatgtcag aaatcgccat tgtgctggat gaagcagaac gtctgaatgt
    26821 cgttcctcgt ctgggcgtgc gtgcacgtct ggcttcgcag ggctccggta aatggcagtc
    26881 ctccggcggg gaaaaatcga agttcggcct ggcggcgact caggtactgc aactggttga
    26941 aaccctacgt gaagccgggc gtctcgacag cctgcaacta ctgcacttcc acctcggttc
    27001 gcagatggcg aatattcgcg atatcgcgac aggcgttcgt gaatccgcgc gtttctatgt
    27061 tgagctgcac aagctgggcg tcaatattca gtgcttcgac gtcggcggcg gtctgggcgt
    27121 ggattatgaa ggtactcgtt cgcagtccga ctgttcggtg aactacggcc tcaatgaata
    27181 tgccaacaac atcatctggg cgattggtga tgcgtgtgaa gaaaacggtc tgccgcatcc
    27241 gacggtaatc accgaatcgg gtcgtgcagt gactgcgcat cacaccgtgc tggtgtctaa
    27301 tatcatcggc gtggaacgta acgaatacac ggtgccgacc gcgcctgcag aagatgcgcc
    27361 gcgcgcgctg caaagcatgt gggaaacctg gcaggagatg cacgaaccgg gaactcgccg
    27421 ttctctgcgt gaatggttac acgacagtca gatggatctg cacgacattc atatcggcta
    27481 ctcttccggc acctttagcc tgcaagaacg tgcatgggct gagcagcttt atttgagcat
    27541 gtgccatgaa gtgcaaaagc agctggatcc gcaaaaccgt gctcatcgtc cgattatcga
    27601 cgagctgcag gaacgtatgg cggacaaaat gtacgtcaac ttctcgctgt tccagtcgat
    27661 gccggatgcg tgggggatcg accagttgtt cccggttctg ccgctggaag ggctggatca
    27721 agtgccggaa cgccgcgctg tgctgctgga tattacctgt gactctgacg gtgctatcga
    27781 ccactatatc gatggtgatg gtattgccac gacaatgcct atgccggagt acgatccaga
    27841 gaatccgccg atgctcggtt tctttatggt cggcgcatat caggagatcc tcggcaacat
    27901 gcacaacctg ttcggtgata ccgaagcggt tgacgtgttc gtcttccctg acggtagtgt
    27961 agaagtggaa ctgtctgacg aaggcgatac cgtggcggac atgctgcaat atgtacagct
    28021 cgatccgaaa acgctgttaa ctcagttccg tgatcaagtg aagaaaaccg atcttgatgc
    28081 tgaactgcaa caacagttcc ttgaagagtt cgaggcaggt ttgtacggtt atacctatct
    28141 tgaagatgaa taattaagat tattctgctg gcgggtcgat ttatttacgt aaatcggtcc
    28201 gctttctttt tatatattct taaaaataat ttagctgctt ttcttattaa ataatctccc
    28261 tacaatcgct gcattaatta aatgcagagg gaacggttat gaaaaaatgg aaagtacgca
    28321 gcgcgttggt ggcattaatc gtgctgctgg ccggttgttc cagtaatgcg cagtataata
    28381 gcagtgcatc gggcaatgtc ggcaccgcct ggggtgggga tgtgcactca acggttcagg
    28441 gcgtatctgc tgagcgagcc tggcgcgatc cggcggagat gatcgttatt agttattcga
    28501 ctaatgttcc ttccggctac gatcgcgttt atagcattcg tattaacgaa ctggaatatg
    28561 ctattcgcga tggtaatttt aattctctac ctattacgcg tgtttatgat tcatctaata
    28621 atgaaccacg ctatattgtc catgccagag tcggaatgaa ttatcaatta tatgtccgta
    28681 attacagtcg taacaccaac tacgagatcg ttgctacggt tgatggcctg gatgtgctga
    28741 acggcaagca aggttcgctg aataacaacg gctatattgt gaatgccgga gattcgttag
    28801 cgattaaagg attccgcaaa gataaacata ccgaagcggc ttttcagttc gccaacgttg
    28861 ctgattccta cgcagccaat tcggcgcaag gcgatgtgcg taataccggt gtaatcggct
    28921 ttgccgcctt tgaattacaa ggccaggcgc aaaacgcgct gccaccgtgc tctggacaag
    28981 cctttccggc agataacaat ggctatgcgc cgccaccgtg tcggaaatag caacctcgct
    29041 tgatgcctcg cccatttaag gcgataatta gcacaaactt gtgtttcgca aatccaaccc
    29101 ttcctcgtcg ggcctaacga cgcggaaggg tttttttata tcgactttgt aataggagtc
    29161 catccatgag caccttaggt catcaatacg ataactcact ggtttccaac gcctttggtt
    29221 ttttgcgcct gccgatgaac ttccagccgt atgacagcga tgctgactgg gtgattactg
    29281 gtgtgccgtt cgatatggcc acttctggtc gtgcgggggg acgccacggt ccggcagcta
    29341 tccgtcaggt ttcgacgaat ctggcctggg aacacaaccg cttcccgtgg aatttcgaca
    29401 tgcgtgagcg tctgaacgtc gtggactgtg gtgatctggt atatgctttc ggcgatgccc
    29461 gtgagatgag cgagaagctg caggcgcacg ccgagaagct gctggctgcc ggtaagcgta
    29521 tgctctcctt cggtggtgac cactttgtta cgctgccgct gctgcgtgct catgcgaagc
    29581 atttcggcaa aatggcgctg gtacactttg acgcccacac cgatacctac gcgaacggtt
    29641 gtgaatttga ccacggcacc atgttctata ccgcgccgaa agaaggtctg atcgacccga
    29701 atcattccgt gcagattggt attcgtaccg aatttgataa agacaacggc tttaccgtgc
    29761 tggacgcctg ccaggtgaac gatcgcagcg tggatgacgt tatcgcccag gtgaaacaaa
    29821 ttgtgggtga tatgccggtt tacctgacct ttgatatcga ctgcctggat cctgcatttg
    29881 cgccgggaac cggtacgccg gtaattggcg gtctgacctc cgatcgcgct attaaactgg
    29941 tacgcggcct gaaagatctc aacatcgttg ggatggacgt agtggaagtg gctccggcat
    30001 acgatcagtc ggaaatcacc gctctggcag cggcaacgct ggcgctggaa atgctgtata
    30061 ttcaggcggc gaaaaagggc gagtaagcac cagatgcgat gcgcacgggt aaaacgtgcc
    30121 attaatgtcg gatgcggcgt gaacgcctta tccgacctac gttcggcacc cgtaggccgg
    30181 ataagatgcg ccagcatcgc atccggcaat gcgcacaagg taacaaatgt gccattcatg
    30241 tcagatgcgg cgtgaacgcc ttatctgacc tacgttcgac accaccaggc tttacttaat
    30301 cccatccgcg ctcatgcgat cgcgaatatg ctgggcgcgt tcggcggatg caggatggtc
    30361 gtcaaacatt gagctttggc gaccttcttc cagttttgcc agtttttcaa agctggtggc
    30421 aagacctgcc gggctgatgc cgcgttggcg cagaagatcg taagaataat catcggcttc
    30481 tgcttcctgg cgctgggaga attgcgaatt aactaatttc tcgcccagat caccgagttg
    30541 tgattgagat aaactgccga caatcccgcc cgccgaggcc gcagctactc gtacggcatt
    30601 tgtaccaagt gccacctgca ttcctttttt cacatggcct aatgccacgt gccccatttc
    30661 gtgaccgatc accgcttcga cttcgttatc cgtcatcata tccatcagcc cgctatagac
    30721 acggatacag ccgttagcca ttgcaaaggc gttcacatcc ttcgccatat acactttgta
    30781 atttaccggc tgaccgttga tattgttgcc tagcgcattg gcaatagttg tcagacgttt
    30841 agcgtattcg ctattggctg gcgcaatcgt cgccttgctg tccatctcct gacatgcctg
    30901 atcgctcagg gttttcacct gcgcatcact caaactgtaa gcctgaaaag cttccgctcc
    30961 tgatgagagc agtccgttgg agtccatatt ctggcaaccg gtcagtaccg ttgccacgct
    31021 cattgctacc aataaggcgc gaattttcat gttttttctt ccgcacaagg tcaggattat
    31081 tctaaaaatg cgccgtttgc aggtgaaccg acgctcagtc tcagtataag gaaaagcgca
    31141 gcggacgggc gagtagattg cgcaacatgc gagcatgatc cagagatttc tgaagcggca
    31201 aaaggatgtt ccatgtacat gacgcgcggc ttgcggtaaa ttgttggcaa attttccggc
    31261 gtagcccaaa acgcgctgtc gtcaagtcgt taagggcgtg cccttcatca tccgatctgg
    31321 agtcaaaatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga gcatggacgc
    31381 agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg acattgccga
    31441 agtcctgtgg cgtgatttcc tgaaacataa cccgcagaat ccgtcctggg ctgaccgtga
    31501 ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc tgcacctcac
    31561 cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact ctaaaactcc
    31621 gggccacccg gaagtgggtt acaccgctgg tgtggaaacc accaccggtc cgctggggca
    31681 gggtattgcc aacgcagtag gtatggcgat tgcagaaaaa acgctggcgg cgcagtttaa
    31741 ccgtccgggc cacgacattg tcgaccacta cacctacgcc ttcatgggcg acggctgcat
    31801 gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc tgggtaaact
    31861 gattgcgttc tacgatgaca acggtatttc tatcgatggt cacgttgaag gctggttcac
    31921 cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg acatcgacgg
    31981 tcatgacgca gcatccatca aacgcgcagt agaagaagcg cgcgcagtga ctgacaaacc
    32041 gtccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag ccggtactca
    32101 cgactcccac ggtgcgccgc tgggtgatgc agaaatcgct ctgacccgcg aacagctggg
    32161 ctggaaatac gcaccgttcg aaatcccgtc tgaaatctat gcgcagtggg atgcgaaaga
    32221 agcaggccag gcgaaagaat ctgcatggaa tgagaagttt gcggcttacg cgaaagctta
    32281 tccgcaggaa gcggctgaat ttacccgccg tatgaaaggc gaaatgccgt ctgacttcga
    32341 cgccaaagcg aaagagttta tcgctaaact gcaggctaat ccggcgaaaa tcgccagccg
    32401 tacagcgtct cagaatgcta tcgaagcgtt cggcccgctg ttgccggaat tcctcggcgg
    32461 ctccgctgac ctggcaccgt ctaacctgac cctgtggtct ggttctaaag caatcaacga
    32521 agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga ccgcgattgc
    32581 taacggtatc tccctgcacg gtggcttcct gccgtacacc tccaccttcc tgatgtttgt
    32641 cgaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc aggttatggt
    32701 ttacacccac gactccatcg gtctgggcga agatggcccg actcaccagc cggttgagca
    32761 ggttgcttct ctgcgcgtga ccccgaacat gtctacatgg cgtccgtgtg accaggttga
    32821 atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggtccgaccg cgcttatcct
    32881 ctcccgtcag aacctggcgc agcaggaacg tactgaagag caactggcaa acatcgcgcg
    32941 cggtggttat gtgctgaaag actgtgctgg tcagccggaa ctgatcttca tcgcgaccgg
    33001 ttcggaagtt gaactggctg ttgccgccta cgaaaaactg actgccgaag gcgtgaaggc
    33061 gcgcgtggtt tccatgccgt ctaccgatgc attcgacaag caggatgcgg cttaccgtga
    33121 atccgtactg ccgaaagcgg ttactgcacg cgttgcggta gaagcgggta ttgctgacta
    33181 ctggtacaag tatgttggcc tgaatggcgc tatcatcggt atgaccacct tcggtgagtc
    33241 agctccggca gagttgctgt tcgaagagtt cggcttcacc gtcgacaacg tcgttgcgaa
    33301 agcaaaagaa ctgctgtaac tagcatttcg ggtaaaaagg tcgcttcggc gacctttttt
    33361 attaccttga tatgtccgtt tgcggacaag caatagataa agcgtgttgt agatcgcaaa
    33421 tatttatatg caataaatat caattatgta atatgcatca cgatatgcgt gttgacattt
    33481 gttgttatat ctataactca atgttatata agaaattaat aattcactgt tttcaaaaca
    33541 ccggtttccc tgctcaattg ctttcattaa accgcacgag tattttattt ttaccgccgg
    33601 acggtaccct aaaaataaaa taaggataat ttatgcggct tagtgactat tttccagaat
    33661 catcaatctc agttatacat tcagcaaaag attggcagga agctatcgat ttctcgatgg
    33721 tatcattgct ggataaaaac tatatcagcg agaattacat tcaggcaatt aaagattcca
    33781 ccattaacaa tggcccttat tatattctcg caccaggcgt ggcaatgcct catgcgcgac
    33841 cggaatgtgg ggcgcttaaa accgggatgt ctttgacatt acttgaacaa ggtgtttatt
    33901 ttccggggaa tgacgaacca attaaattac tcatcggact ctctgctgcc gatgccgatt
    33961 cgcacattgg cgctattcag gcgttaagtg aattactgtg cgaagaagaa atactcgaac
    34021 aactcttaac agcatcatca gaaaaacaat tagcggacat tatcagccgc ggataattac
    34081 tctctcctct acaggataat attatggaaa acaagtctgc tcgtgcaaag gtccaggctt
    34141 ttgggggctt tttgactgca atggtcatcc ccaatattgg tgcttttatt gcctggggtt
    34201 ttattactgc gttatttatt cccaccggtt ggctgcctaa cgaacatttc gccaaaattg
    34261 tcggcccgat gattacctat ttattgcccg tgatgattgg ttctaccggt ggtcatctgg
    34321 tcggcggtaa acgcggcgcg gtcatgggcg gaataggtac tattggtgtg atcgtcggcg
    34381 cagagatccc gatgttcctt ggctcaatga ttatggggcc gctcggtggt ctggtcataa
    34441 aatatgttga taaggcactg gaaaaacgca tacctgccgg ttttgagatg gttatcaata
    34501 acttctcatt aggtatcgcg gggatgctcc tttgcctgct gggttttgaa gtgatcggcc
    34561 cggcggtgtt aattgccaat actttcgtca aagagtgtat tgaggcgctg gtacatgcgg
    34621 gttatctgcc tctgctgtca gtcatcaatg aaccggcgaa agtgcttttc cttaataatg
    34681 cgatcgatca gggcgtctat tatccgctgg gaatgcaaca ggcttcggtt aacggtaaat
    34741 ccatcttctt tatggtggcc tctaacccag gtccgggcct ggggctgctg ctggcgttta
    34801 ccttgtttgg taaagggatg agtaaacgtt ctgcgcccgg ggcgatgatt attcacttcc
    34861 tcggtgggat ccacgaactg tatttcccat atgtgctgat gaagccgctg actattattg
    34921 ccatgattgc gggcggtatg tctggcacct ggatgtttaa cttactggac ggtggtctgg
    34981 tggctggccc aagtccgggg tctatctttg cttacctggc gctgacgccg aaaggttcgt
    35041 tcctggcgac aattgccggt gttacggtag gtaccctggt gtcctttgct atcacttcgc
    35101 tgatactgaa gatggaaaaa acggtggaaa cggagagcga agatgagttt gctcagtcag
    35161 ccaatgcggt taaggcgatg aaacaagagg gtgcattctc gttaagcagg gttaagcgta
    35221 tcgcctttgt ttgcgatgcg gggatgggct ccagtgcgat gggcgcgacc accttccgta
    35281 aacgcctgga aaaagcgggg ctggcaattg aagtaaaaca ttacgccata gaaaacgtgc
    35341 ctgcggatgc ggatatcgtc gttactcatg ccagtctgga agggcgcgtg aaacgtgtga
    35401 cggataaacc actgatattg attaataact atattggcga tccaaaactc gacactttat
    35461 ttaatcaatt aaccgccgaa cataaacact gattacagag gtaaaaatga aaaccaaagt
    35521 tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc cagaaattac
    35581 cgataatgaa ttactggtga gtgtgatttc tgacagcgtc tgtttatcca cctggaaagc
    35641 ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc atccggtcat
    35701 taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca ctggcaaata
    35761 taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg gatattcagc
    35821 gggctacagc tacgaatatt ttggcggcaa tgccacttat atgattattc ccgaaatcgc
    35881 cattaatttg ggctgcgtat taccgtatca cggctcttat tttgctgcgg cgtcgctggc
    35941 agagcctatg tgctgcatta ttggcgctta tcatgccaat tatcacacca cgcaatatgt
    36001 ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg cgtgtgcagg
    36061 tccgatgggc attggcgcta tcgattacgc cattaacggc ggcatacaac cgtcgcgggt
    36121 ggtggtggtc gatatcgacg acaaacgtct ggcgcaggta cagaagctgc tgccggtgga
    36181 actggcggcc agtaaaggca ttgagctggt gtatgtgaat accaaaggga tgagcgatcc
    36241 tgtccagatg ctgcgggcgc tgacaggaga tgccgggttc gatgacattt ttgtttatgc
    36301 ggcggtgtct gctgtcgttg agatggctga tgaattactg gcggaagatg gctgtctgaa
    36361 cttctttgcc gggccgacgg ataaaaactt caaagtgccg tttaatttct acaacgttca
    36421 ttacaacagc acgcacgtcg tcggtacatc cggcggttca acggacgaca tgaaagaggc
    36481 gattgccctt agcgccacag ggcagttaca gccctcgttt atggtgaccc atatcggtgg
    36541 cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat atccctggcg gtaaaaaact
    36601 catttataac ggcgtgacca tgccgctcac tgccattgcc gattttgccg aaaaaggcaa
    36661 aaccgatccg ctgtttaaag agttggcgcg gctggttgag gaaacgcacg gcatctggaa
    36721 tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatatcgggg aggccgcgca
    36781 atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt ggctgcctgg
    36841 ccgcaaaccg gatgtggcga caaaaataaa attgatggcc tggcggtcac cgcgatgcgc
    36901 caggcattaa acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg agaaattgac
    36961 catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc agaagtcgat
    37021 atcgcggttg atcccattga aggcacgcgg atggtggcga tggggcagag caatgcgctg
    37081 gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg cgcccgatat gtatatgaaa
    37141 aagctggttg ttaatcgact ggctgccggg gcgattgatc tgtctttgcc tctggcggac
    37201 aacttgcgtg acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg catggttacg
    37261 ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa cacagctggg cgtgaaggtt
    37321 tttgccctgc cggatggcga tgtcgccgcc agcgtgctga cctgctggca ggataatcct
    37381 tacgatgtga tgtacaccat cggcggtgcg ccggaaggcg tgatttctgc ctgtgccgtt
    37441 aaagcattag gcgggaatat gcaggccgag ctgattgact tttgtcaggc caaaggggat
    37501 tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgcaaggc aatgggtgtt
    37561 gacgttaacc gtgtgtactc gctcgatgta ctggtaaggg ggaacgatat cctctttagc
    37621 gctacgggcg tcacgggcgg cgagttggtg aacggtatcc aacagacggc gaatggagtg
    37681 cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat agactccctg
    37741 tattgatggt gatctatcgg aagaccaatg gcgacgctgt cagaagatga tgtgcttgag
    37801 caactggatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc tattttgctc
    37861 caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga gagcgtcgaa
    37921 tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga tgttgcactg
    37981 cgtttgattt atgcgctggg gaaaatggat aaatggctgt acgccgatat cacgcatttt
    38041 tcccagtact ggcattacct gaaagaacag gatgaaacgc tcggatttgc cgatgacatg
    38101 acttgggatt ttatcagcaa cgtcaatagc ataacctgca atgcgacgct ctatgacgcg
    38161 ttgaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt tagcggaatg
    38221 gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac gccgtgaaaa
    38281 ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa gagatagaac
    38341 gcgtgcataa accgttgctg cgtatgctgg cggccttaca gacggtaaat ccgcagcgcc
    38401 gaaccgtggt ttttctttgc gctccgccgg ggacgggtaa atctacccta accaccttct
    38461 gggcatatct cgcgcaacaa gacccggagc tgcccgctat tcagacgctc ccgatggatg
    38521 gttttcacca ttacaatagc tggctggatg cgcatcaatt gcgccccttc aaaggcgcac
    38581 cagagacatt tgacgttgcg aaactggcgg aaaatctgcg ccaggtcgtg gaaggggatt
    38641 gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt tgaagatgcg ttgcacgtta
    38701 ccgcaccaat aatcatcgtc gaaggaaact ggctattgct ggatgacgat aactggcgcc
    38761 agctagcgca attttgtgat ttctcgatct ttatcaatgc gccagctact gcgttgcggg
    38821 aacgactggt ggggcgcaag ctggcgggag ggttatcgct ggcagacgca gaagcttttt
    38881 atgaccgtac cgacgggccg aatgttcgcc gggtgctgga agagagtctg cctgcaaatt
    38941 tgaccttaat gatgaccgct acgggtgaat atcgtctggt ggattaatgt tcctgattag
    39001 accatcttcc agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg cgtcatgact
    39061 gattgctacg acgctcgtgc ctcgctggcg gcacttctcc agccagcttt caaacacgct
    39121 caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc agcaatagtg gcggagagag
    39181 tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ctatgcagat ccaacgggtg
    39241 cgcgctggca gtgtctgcta actgacaaca ttgcagggca gcatgggtac gttgagtgat
    39301 ttcatccgcc gggcattttt gcagtttcaa accaaatgcg atctcgtcag cgacggtgct
    39361 gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca atctttgccg cgcgctggcg
    39421 gtttttcagg tttttcatcg cctgttgttg cagcataacc gtaccggctg tgggcgtaag
    39481 aagacctgcc attactcgta acagcgtcga tttccctgcg ccattgtcgc cggtcaacgc
    39541 caaccattca ccttgtttga gttgcagaga aatgtcgcaa agacaatccg ttgccgcgcc
    39601 aggccagcga taagatatct gatttaacgt taacatagtg gctggagcgc tccgttttgc
    39661 aattgccacg cttgctggca gtagttcagg aaaggtgtgc gatgacgttc aaaaagaata
    39721 atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct gcaacagcat ttcgctggcc
    39781 tgcggcgtca ggcggctaaa ggcttcatca agaattaata gctttggctg catcgcgatg
    39841 gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga gtgttgccgg gtgacgatgg
    39901 cgcaacggct ggcactcggt cagagtcagg gcggcatcaa tccgcgccat aatctctgct
    39961 tcagcaagac acagattttc tggcccaaag gcaacttctt cttccacgct aaaggtacag
    40021 ccggagagct gcaaataggg cgattgctgg acgagctgga tggtggcgga ttgttcatta
    40081 agcgggagat gcccaatggg cgtaccgagg agcgtccccg tgcctgttat ctctcctggc
    40141 agaaagtccg gataccagcc cgccatcagc tgtgccagcg tacttttgcc actgccgttg
    40201 tcaccaaaga tggcgaccat ccccggtttg acatagtgaa agtcataaca gaacggagga
    40261 tgcgttgagt gtgtcgggca ataacggaac tgttctaacg taaccatatc cacgctccga
    40321 attctgtcag catcagcaag atcatggtgt aacgcgctac gcgttgtagg gtgctatcgg
    40381 ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa tgcccgcata tccagcgccg
    40441 caccacggac agcaagatcg tttagcgcat tgtgtgttag cggaatagtt aacgcgggca
    40501 tcgcacgtaa acgctgatac cagccctcat ccagcggcac gccgcgggca cgctgcgctt
    40561 catgaataat cgctaattgc cgttttaact gctcaacgac caataacggc ccggcaaaca
    40621 gataagcgac gcccggcggc aggcgagaag caaacagagc gcggataaaa cattgcacgg
    40681 ggacgtactg catccacaac tgtgaggttg aaacaatcgc cagaatccgc aaccagagcg
    40741 taatagcatg cgtccaacgc tccggcgaac gcggcgtgcc gcttagccat tctgttagcc
    40801 agccaccgtg taccagccac agcccggctc ccagcgagaa catcagccag acgacatatt
    40861 ttgcccgccg ccgcgtggcc tttagcgcaa taaggcaaaa aaaggtcgcc gcgctataga
    40921 tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca ggccgccaat gcccatagtg
    40981 ttaatgaggt aaatggatgc atttagcgca cggcagccat tgccggaaaa tggcgcgtgg
    41041 tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac cgctgtaaga gttttatcga
    41101 ccaggttcgc gcctatcacc gtaatcgcca ccgactccac caggttttgc cccatcgagt
    41161 gcatccaggc aacgaacaaa tcggctccgc taccggtgac accgccgaac agcgcagtac
    41221 gcagcggtac agcgacgacc gttaccgcaa gcgtaataat cacgccgctg acaacgactt
    41281 ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc ggcgaccagg ccgatgacca
    41341 tcgcgacggg ggcaaaagcg gcagcgatcg ggtcggtcag taatccccag agtaaattgg
    41401 tcagtaagcc ggtcagcatc ccgatcaccg gaccaagcag taccgcgcta ataagtgtgc
    41461 cgatggagtc gagaaatata ggtaatttga ccatgctggc cagttgcccg ccgatcatat
    41521 tgatggcgat agatataacg atcaacacca gtgcctgact ggaaaagtgg ctacgcgcca
    41581 taagaaaacc tctctgaaag tagttattta acgggggaaa tatgcaactc gtcgtaacca
    41641 gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa ggctaccgat caccagttca
    41701 agcgtggtcc gcacggtaca gccgggcatc atatgaccgc caagcatcgt gccgtgtgga
    41761 tcggaaacgc agagatgaag atgctcacca ctctgttcca acgtgccgtt tagtgagatc
    41821 acttcgaatg taccgttcag gagcgtggtg ccttcttgcc cggcatagcg caaggcgatg
    41881 tcagtcaggc taccggtaca gcctgcaatc caggcggcat gaagttgctg ctgctgtgca
    41941 aaagcgtgta gctgggaaaa aacttcctgc ccggggagga ggcgcagtgc gtaaaagcgt
    42001 gcggaggaag aatatgggta gggcaatgtc atcattggag tactccgaaa ctcggacgta
    42061 ttacggataa tatcgacgct gcatcagtca tattctgata taagtcaggg tttgtgacgg
    42121 cacaacgaaa agattttgct acgcttattc gtgattctgc aaagtggaat aaattccaca
    42181 ggggcggggc tgaaaacgtg acgtaagtca gataattgtt ttctttgact ggacaatcat
    42241 tccttttatt ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt aaatgttcga
    42301 caattaacca atcagtcgca gtttgcgaca ggtaaggttt ccccggacga tttgctggat
    42361 tactctgtct ggcaaatttg ctcagggaaa accttgcagg agatctatga ccgtacgcgt
    42421 agcgataaat ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt atgaatccgg
    42481 acgccgggcg gaaattaccg tggtggcaat caacgaactg gcggatgctg cgggcatggc
    42541 gcatttgttg aaatacgaca ccagccatgg ccgttttgca tgggaagtac gacaggaacg
    42601 cgatcaactt tttgttggtg atgacgccat ccgcgtattg catgaacgtt cactgcaatc
    42661 gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac tgcaccggcg tatatggctc
    42721 ccgcgagcat ggcgaagcgc acattgccgc cggggctaaa aaagtgctct tttcacatcc
    42781 tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc aatcaggatc aacttcgtgc
    42841 ggagcaccgc atcgtttcta acgcttcctg taccacgaat tgcataattc ccgtcatcaa
    42901 attgttagat gatgcgtacg gtattgagtc cggcactgtg accacaattc actccgccat
    42961 gcacgatcaa caggttattg atgcatacca tcctgacctg cgtcgcaccc gggcagccag
    43021 ccagtcgatc attccggtcg atactaaact ggccgccggt atcacacgat tttttccgca
    43081 atttaacgat cgctttgaag cgattgcggt acgtgtgcca accataaatg tgacggcaat
    43141 cgatttaagc gtgacggtga agaaacctgt aaaagccaat gaagtcaacc tgttgctgca
    43201 aaaagcagca caaggtgcat ttcatggtat agttgactat acggaattgc cgttggtctc
    43261 tgtagatttt aaccacgatc cgcacagtgc cattgtcgat ggcacccaaa cccgggtcag
    43321 tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac gaatggggct ttgctaaccg
    43381 aatgctcgac acgacgttag ctatggctac tgttgctttc aggtaagacg caagcagcgt
    43441 ctgcaaaact tttagaatca acgagaggat tcaccatgtc tgtaattaag atgaccgatc
    43501 tggatcttgc tgggaaacgt gtatttatcc gtgcggatct gaacgtacca gtaaaagacg
    43561 ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa ctggccctga
    43621 aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa ggcgagtaca
    43681 acgaagaatt ctctctgctg ccggttgtta actacctgaa agacaaactg tctaacccgg
    43741 ttcgtctggt taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa ctggttgttc
    43801 tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga cgaaatcctg tccaaaaaat
    43861 acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg tactgctcac cgcgcgcagg
    43921 cttctactca cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg ctgctggcag
    43981 ctgaactgga cgcgctgggt aaggcactga aagaacctgc tcgcccgatg gtggctatcg
    44041 ttggtggttc taaagtatct accaaactga ccgttctgga ctccctgtct aaaatcgctg
    44101 accagctgat tgttggtggt ggtatcgcta acacctttat cgcggcacaa ggccacgatg
    44161 tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc aaaacgtctg ctgaccacct
    44221 gcaacatccc ggttccgtct gatgttcgcg tagcaaccga gttctctgaa accgctccgg
    44281 ctaccctgaa atctgttaac gacgtgaaag ctgacgagca gatcctggat atcggtgatg
    44341 cttccgctca ggaactggct gaaatcctga agaatgcgaa aaccattctg tggaacggtc
    44401 cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg gctaacgcta
    44461 tcgcagacag cgaagcgttc tccatcgctg gcggcggcga cactctggca gcaatcgacc
    44521 tgttcggcat tgctgacaaa atctcctaca tctccactgg cggcggcgca ttcctcgaat
    44581 tcgtggaagg taaagtactg cctgcagtag cgatgctcga agagcgcgct aagaagtaaa
    44641 aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc ttttagagca cgcatttttt
    44701 catactctaa ataattcgag ttgcaggaag gcgacaagag agtgaatccc caggagctta
    44761 cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt gaagtatgac
    44821 gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga tttcgtaaaa
    44881 cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa agaaaacaac
    44941 ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt actggaaact
    45001 gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc ttcctttatc
    45061 gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg ctatcctggg cgcgatctct
    45121 ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat cctgcacact
    45181 gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc gggtgaaaaa
    45241 cacttcgcag ctaccggtaa gccgctgttc tcttctcaca tgatcgacct gtctgaagaa
    45301 tctctgcaag agaacatcga aatctgctct aaatacctgg agcgcatgtc caaaatcggc
    45361 atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt ggacaacagc
    45421 cacatggacg cttctgcact gtacacccag ccggaagacg ttgattacgc atacaccgaa
    45481 ctgagcaaaa tcagcccgcg tttcaccatc gcagcgtcct tcggtaacgt acacggtgtt
    45541 tacaagccgg gtaacgtggt tctgactccg accatcctgc gtgattctca ggaatatgtt
    45601 tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg tggttccggt
    45661 tctactgctc aggaaatcaa agactccgta agctacggcg tagtgaaaat gaacatcgat
    45721 accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc gaacgaagct
    45781 tatctgcagg gtcagctggg taacccgaaa ggcgaagatc agccgaacaa gaaatactac
    45841 gatccgcgcg tatggctgcg tgccggtcag acttcgatga tcgctcgtct ggaaaaagca
    45901 ttccaggaac tgaacgcgat cgacgttctg taagtcattc ctttctgctt atctcaaggc
    45961 ccgctctgcg ggtctttttt tcgccaaaag caaaattgcc tgatgcgcta tgcttatcag
    46021 gcctacacgt atcctgcaat atattgaatt tgcaggtttt tgtaggccgg ataaggcgtt
    46081 cacgccgcat ccggcatgac aaagcgcaca ttgatatctg tctgagcccc gctctgcggg
    46141 gatttttttg ccaaaagcaa ggtgattcag atgagaaatc tgtgatctat ttggcaaaat
    46201 tatgctttat tgtttaccct tgtcagactg cccgtcataa ggcggcggag tgtatttctc
    46261 cattttgagt cagttgaaaa ggaatattga atggaagatt tgaatgttgt cgatagcata
    46321 aacggcgcgg gaagctggct ggtagctaac caggcgctgc tgctaagtta tgcagtaaac
    46381 atcgtggcgg cactcgcgat catcatcgtt ggtttgatta tcgcgcggat gatttccaac
    46441 gcggtgaatc gcctgatgat ctcccgtaaa atcgatgcca ctgttgctga ttttctttct
    46501 gcattagtcc gttacggtat tatcgccttt acgctaatcg cagccctggg acgcgtgggt
    46561 gtacaaacag cgtcagtcat tgctgtactc ggtgccgcag gcttagctgt tggtctggct
    46621 ttgcaggggt cactttctaa cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc
    46681 cgtgccggag aatatgttga cctcggcggc gtagccggta ctgtgctgag tgtgcagatt
    46741 ttctccacca ccatgcgtac tgcagacggt aaaattatcg ttattccgaa cggtaaaatt
    46801 attgccggaa atattattaa cttctcccgc gagccagttc gccgtaacga atttattatt
    46861 ggcgtggcgt atgattccga tatcgatcag gttaagcaga tcctgaccaa tattatccag
    46921 tctgaagatc gcattttgaa agatcgcgaa atgactgtgc gcctgaacga acttggtgca
    46981 tcgtcgatta atttcgtggt ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac
    47041 tgggatgtgc tggagcgtat taaacgtgaa tttgatgccg ccggtatcag cttcccgtac
    47101 ccgcaaatgg atgtgaactt taagcgggtg aaagaagaca aagctgcgta atcaacgctg
    47161 aggccagata atactctctg gcctctctct tattagttat tctgattgcc aattaatatt
    47221 atcaatttcc gctaataaca atcccgcgat atagtctctg catcagatac ttaattcgga
    47281 atatccaacg tgttttctta ttactttcaa ggtcttgctc ttggggcagc tatgattctg
    47341 ccgctcggcc cacaaaatgc ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt
    47401 atgattgcct tactttgcgc tatcagcgat ttggtcctga tttgcgcggg gatttttggt
    47461 ggtagcgcgt tattgatgca gtcgccgtgg ttgctggcgc tggtcacctg gggcggcgta
    47521 gtcttcttgc tgtggtatgg ttttggcgct tttaaaacag cgatgagtag taatattgag
    47581 ttagctagtg ccgaagtcct gaagcaaggc agatggaaaa ttatcgccac catgttggca
    47641 gtgacctggc tgaatccgca tgtttaccta gatacttttg ttgtactggg cagccttggc
    47701 gggcagcttg acgtcgaacc aaaacgttgg tttgcgctcg ggacaattag cgcctctttc
    47761 ctgtggttct ttggtctggc tattctcgca gcctggctgg caccgcgcct gcgcacggca
    47821 aaatcacagc gcattatcaa tctggtagtg ggatgtgtca tgtggtttat agccttgcag
    47881 ctggcgagag acggtattgc tcatgcacaa gccttgttca gttaggcgct atctgatgga
    47941 aaaataaaac agaggcgcta agcttgcctc cagagatcct gaattttggg caagtcaatt
    48001 aacacggaga gactaacgtg aagttcaaag ttatcgccct ggcggcatta atgggtatta
    48061 gcgggatggc agcgcaggct aacgaattgc cggatggacc gcatattgtc acctccggta
    48121 cggcaagtgt ggatgcggtg ccagacattg ccactcttgc gattgaagtt aacgtggccg
    48181 cgaaggatgc cgctacagcc aagaaacagg cagatgagcg cgtcgcacaa tacatttcct
    48241 tccttgaact caatcagatc gcgaaaaaag atatcagctc agcgaattta cgcacccagc
    48301 cagattatga ttatcaggat ggtaaaagta tcctgaaagg ctaccgcgct gtgagaacgg
    48361 tggaagtcac gctccgtcag ttagacaaac tgaattcctt gctggatggc gcgctgaagg
    48421 cgggtcttaa cgaaattcgt tcagtgtcgc tgggcgtggc gcagccggat gcctataaag
    48481 acaaagcgcg taaggcagcg attgataacg cgattcatca ggcgcaggaa ctggcgaacg
    48541 gctttcatcg taaactgggg ccggtatata gcgtgcgcta ccatgtttcc aactatcagc
    48601 ccagcccaat ggtgcggatg atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt
    48661 acgagcaggc cgctattcag tttgatgatc aggtcgatgt ggtcttccag ttagaacctg
    48721 tggatcaaca acccgctaaa acacctgcag cacaataata cttgtagccc gacgtattcg
    48781 tcgggctaat gcctttaccc gatattgcct cctttttctg taattttctg taaaaccatg
    48841 acgtaatcac ctatctttgc tcgcggtttc atcaaggata ggctgactga aacggttaag
    48901 cggacaagga tgcggggagg ggggatggac atttttattt cgaaaaagat gcgcaatttc
    48961 attttattag cgcaaaccaa taatattgcc cgggcggcag aaaaaatcca tatgacggct
    49021 tcgccatttg gtaaaagcat tgccgcgctg gaagaacaaa ttggctatac gctatttacc
    49081 cgcaaagata ataatatcag cctcaataaa gccggtcagg aactgtatca aaaactgttc
    49141 ccggtttatc aacgactttc tgcgattgat aatgaaatcc ataactcagg gcgtcgttca
    49201 cgagaaattg tgattggcat agataatact tatccgacaa ttattttcga tcagttgatt
    49261 agtctcggag ataagtatga aggcgtgacc gcccagccag ttgagttcag cgaaaacggc
    49321 gttatcgata atctttttga ccgtcagtta gattttatta tctccccaca gcatgtatct
    49381 gcacgagttc aggaactgga aaacctgact atcagtgaat taccaccgct gcgattgaac
    49441 tttctcgttt ctcgccgtta tgaagagcgt caagagcaag agctgcttca ggagctgccc
    49501 tagctacaaa tgcgctttca aaaccgggct aacttcgaag caatgattga tgcgaatatg
    49561 cgaccttgtg ggatcaatcc caccattatt tatcgtccct acagttttat ggccaaaatc
    49621 agcgccgtgg agcgcggaca ttttctgacg gtgatcccgc attttgcctg gcgactggtg
    49681 aatccggcaa cgctgaaata ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat
    49741 ctttattcaa tcagaaatca tcgttatacc gccacgatgc ttcagcatat tgctgaagat
    49801 cgtgacggga caaaccatta acccagcatc gagccggttg caattaaatt acggtgtaag
    49861 tcgaagacgt ggctaagatc gtggtgaata tgtccgccgg gcgcattttc cagatagcga
    49921 tgcagataat cccgatacat aggatgtgca caattatcaa tgatagtgcg ggcgcgttga
    49981 agcggggaaa gaccgcgcag atcggcgatc ccttgttcgg tgatgatcac tttgacgctg
    50041 tgttcgttgt gatcaacatg gctgcacatt ggcacgacgg ttgagatctt gccttcttta
    50101 gcaatcgacg gagccataaa gatcgacaga tacgcgttgc gttcaaaatc accgctgccg
    50161 ccgatgccgt tcatcagatc gaccccggct acgtgcgttg agttggcatg cccgtaaata
    50221 tcaaactcca ggccgacgtt cagagcgatg acgcccagac gacggatgat ttccgggtta
    50281 ttggaaatct cctgcggacg caacacaatg cggctggcaa agtaatccat attgtcgtaa
    50341 atcttgcgca gggaatcggc cgagattgtc aggctggagg cgctggcccc gctgattttg
    50401 ccggtttcca gtaaatgcac caccgattcc tgtagcactt ccgaatacat cataaacgga
    50461 ggaatttccg ggttttcccc cagacgcgcc attaccgcat tattgatatt gcccacgcca
    50521 ctttgcagcg gcagaaattc cggcggaata cgcccatgcg ccatttcctg caataagaac
    50581 gtgaccacgt tatcggcaat ctgctggcac atgggatttt gcttatccag catattaccg
    50641 gcgtcgggca agttggtttc cacgacggcg acaatctttt tcggatcgat ttgcacatag
    50701 cgggtaccga cgcgatccat tgcatggaag atcgacacgc tattgcgccg cggtggcgcg
    50761 ccaggaatca caatatccgc cagttctgca acgcgcggat cgtgatagtg attgagttca
    50821 atgatcactt tcttcgctcg cagcagccag gtcggcgcat taccgatccc gctggttaac
    50881 cagactcgac catctggtgc cagtgccgat gcttcaatga cggcaacatc aatgtcgccg
    50941 aagaaaccgt aattgaccat ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc
    51001 gcgccctgat tgatcttttt acgtaaaccg gacgatgttt gatatggcgc acgccaggaa
    51061 acagcatcgg cgtcagaaag tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc
    51121 aggcgaattt gatacggctt tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg
    51181 gtgggtaggg ctttcggcga acccgctggg gtaaagccgc taaatgccac catgtcgtta
    51241 tgctggataa tttctgccgc ttcatcggcg gtcatccttg tccactgagt ttccattttt
    51301 actccttcgt tcgcagggat taatgaccaa cgaaattagg tttacgtttt tcgaggaaag
    51361 cgttcatccc ttcctggtaa tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac
    51421 gttcaaattc atcggagttc atggtgtgtg cttcgcccag tacacgcagc tcttctttga
    51481 taacggcaat ggctaacggc gctttctcag agatgtggtg cgccatttgt aaggtgaaat
    51541 cttccagttc ttccacttcc acaacatggt tgaggatgcc gacagccagc gcgcgctggg
    51601 cggtgatggg cgaagcggta aaaatcagct ctttgacaat gtggaagccc gcgtcgcggg
    51661 tcaggttgtg aatgccgacc aggttatacg ggacgccgag gtttacaggc gtcattgaga
    51721 aggttgaggt actggcggcg ataatcagat cggaactcat gatcatttca aatgcgccac
    51781 cccaaacact accttccacc atcgaaataa tcggtttcgg gaatttttgg atcatgcggg
    51841 tgatttgacg caatggatca tcataggaga gcggatcgcg accgccagac ggcagttcgt
    51901 gaatatcgtg acctgcggag aagactttgg atccactcgg cgcgcgcaaa atgatacagc
    51961 gaatttccgg ccggttgaga tcgcttaacg cctgcataag atcatcaata aagactttac
    52021 ttaaggcatt aagttttcgg ccatagttaa actcaatgac cgccactttg ttgatagtga
    52081 caacgttaac atactgataa gacataaaaa ttcctttaat caaaatattg cgtctggata
    52141 aattcactga gctgccgcag gccggtgcgc ggtgagagcg tattgttttt gaccgctaaa
    52201 agcgtctggc ggtaatagcg atcgaaatct tcattcgcga acaggtgatt cagtacttct
    52261 tcttcggtct gcttacgcag ccattccacc gattgttgtt gccgcacttg ttgtaaacga
    52321 ccactggcag ttagcgcggt tttgaagtcg atgatggcgt gccagatctc atcgattcca
    52381 cgtttttcca gtgcgctaca agtcagaacc cgtggctgcc attcgtcgta tttacgtcgc
    52441 agaatatgca gggcactctc gtacatatgc cgggcaatgg cgacattggt atggttatcg
    52501 ccatcgtctt tgttgataac gatcagatca gccacttcca tcagcccttt tttaatgccc
    52561 tgcagatcat cgccgccacc ggcaatttgc aacgagataa aacagtccac catgcgggcg
    52621 acttctgttt ccgactgccc gacgccaacc gtttcgacaa tcactacgtc ataacccgct
    52681 gcttcgcata acagcattaa ttcccgcgct cgctgactgg caccgcccag atgaccggag
    52741 gatggtaccg ggcgaataaa cgccgcttcg gcacgcgcca ggtcattcat gcgggtttta
    52801 tccccgagaa tgctaccgcc agtgaccggg ctgctgggat cgaccgcaat aaccgcgacc
    52861 tttaatccct ctcgaatcaa caacatgcca aaggcctcaa gaaaggtact tttccccgcg
    52921 ccgggggtgc cggtaacgcc cagtcgcagg gtgttaccgc agtacggcat aatggcatca
    52981 agcagctgcg tacttagtgc ctgatgacgc gggtgacggc tttccaccag cgtcatggcc
    53041 tgggcgagtg tggcatgctc accctgacgt aagcggcgaa tactttctgc cagcgtggct
    53101 tcattaatca tgatgctggc ttatcagatt cagtacgtcg cgcacactgt cgagcatagg
    53161 tgtacctgga ccataaatcg ccgccacgcc gcgctcttgc aggaaggcgt aatcctgcgg
    53221 tggaatgacg ccaccggcga ctacgcagat atcttcgcgt ccccattttt tcagcgcttc
    53281 gaccagttcc gggatcagcg ttttatgacc ggcagccagt gaggatgcgc ccactacgtg
    53341 aacgtcattt tctactgcca ggcgggcgat ctcttcaggt gtagagaaca tcgggcttaa
    53401 atctacgtcg aaaccgagat cggaataggc gctggcgatc actttcgcgc cgcgatcgtg
    53461 tccatccagg cccattttgg cgatcagaat gcgcgggcga cgaccattgt cggcaaggaa
    53521 ctgctccgtt tgcgcaacaa tggcatcgaa ctcggaggcc gatttctcag attgatgata
    53581 gctttgcgca atcacgccgg taacacactg gcttggcacc agataacggt cgaaagcggc
    53641 ttccagcgca tcggaaattt cacccagggt ggcgcgaacg cgagcggcat taacagcggc
    53701 agccagcagg ttttcgttat gctgtgcggc gtgagtcagg gcgttcaacg cggcggttac
    53761 ggcggcatca tcacgggtgg cgcgaatgcg ttccagcgaa gcaatttgct cgttacgcac
    53821 catcacgttg tcgatctcaa gtacatcggt ttcgtcttcg tgatccagtt tgtacttgtt
    53881 gacaccaacg atgacacgct tgccctggtc gatcagcgac tgttcgcgcg ctgaggcctc
    53941 ttcgatcatt cgttttggca gacctgcttc gatcgctttc gccatgccac cggcttcgtc
    54001 gatctgttgg ataatagctc tggcttgttt gacgatttga tcggtcagcg actcaatgta
    54061 ataggatccg gccagtggat cgacggtgcg gcagagttct gattcttcct ggatgatgat
    54121 ctgggtgttg cgggcaatgc gtgctgagaa atcggtaggc aaaccaagcg cttcgtcaaa
    54181 ggcgttggta tgcagtgact gagtaccgcc cagcgtcgca gccagcgctt caatggtggt
    54241 gcggataacg ttgttatacg gatcctgttc agtcaggctc cagcctgagg tctggcagtg
    54301 ggtacgcagc gccagtgatt tcgggtcctg tgcgccaaat ccactgaccg cttcgctcca
    54361 taaataacgt gccgcacgca acatggcgac gttcataaac agatccatgc cgatgccgaa
    54421 gaagaacgac aggcgaggag cgaagtcatc aattttcagt ccggcagaga ttgctgcttt
    54481 gatgtactca atcccatcag cgagcgtaaa tgctacctgc tgcacgcagt tggcacccgc
    54541 ttcacccatg tggtaaccgc tgatactgat ggtattaaat cgcggcatgt tgccggaaca
    54601 ccaggcgatg atgtcggcga taatgcgcat tgacggtttt ggcgggtaaa tataggtgtt
    54661 gcggcagagg tactctttga gaatatcgtt ttgaatggtg ccggtcagtt tatcaggtgt
    54721 aacaccttgc tcttccgcgg cgatgatata aaacgccagt actggtagca ctgcgccatt
    54781 catggtcatc gaaaccgaca ttttatccag cgggatctgg tcgaacagga ctttcatatc
    54841 ttccacggtg tcgatagcga cgcccgtttt gccgacgtca cccgccacgc gtgggttatc
    54901 ggagtcgtag ccacggtggg tggcaaggtc aaacgcaacg gaaagacctt tttgcccggc
    54961 ggccaggtta cggcgataaa aagcgttgga ctcttttgct gttgaaaaac cagcatactg
    55021 acggatggtc cacggttggg cggtatacat agtggcacgc gggccacgaa cgtagggcgg
    55081 caaaccagga agggtacctg tcacctccag attatcgaga tcggcttcgg tatacagcgg
    55141 cttgatggcg atcccttccg cggtttgctg aaccagcgag tcgacagttt tctcccgacg
    55201 gctcaattcc ttgttggcaa gctgttgcca ctcctgctcg ttagacatta tttgctccgt
    55261 aaaacgcaat caaaatgagg gtatttgcgc ttacggtgag ctttttacgt gggtttgtcg
    55321 ccagcaaaaa aaatgcattc agacgtcgtt ttttgccgcc tgagatttgt ctattttgtg
    55381 atgtgattca aggaatacaa aaagaagtga ggataacgcc tgatatgtgc tatcaggcat
    55441 tatttgatgg attaatcctg acgaaggact ttgtgaccat aatcgagcaa cgcatcagtg
    55501 actttacgca tcatgcggct ttcaggagca aagcggtgcc agtagagcat ccggcgttga
    55561 aatagccctg gcgttaagtc aatcagttca ccgctggcta gttctttctc gatttgcagg
    55621 tgcgggatca tacagcaggt ggtgccctgg cgagcaagtt gcacgaacgc ttctgaagaa
    55681 ttaacgatat ggcagggcac gctgcctgga ggcagatcga agttttgctg caaaaaggcc
    55741 tggtgcatat cgtcaagatg gtcaaacgcg accactggcg ctttcagtaa tgccgaacgc
    55801 gttacgccgt tagggaaata tttttcggca aagggttttg agctgacgaa cagatagtcg
    55861 agcgcaccaa gtttatcgac aagacaactc ggcagcgcct gatgttgaat actcaccgcg
    55921 ccgaccactt cgccgcgacg cagacgttcc tgagtgcggg tttcatcttc tacctgcaag
    55981 ttgaggcgga taggcgaatc agccaataca ggagccagtg ccggaagcaa ccacgtcgcc
    56041 agactgtcgg cattgaccgc cagtgaaagc agcagcggag tcgaaccggt ttgttcatcg
    56101 cccagccact cttcttccag caactccacc tggcgcagca gtgccagcag tttttgccct
    56161 tgttccgtcg ggcgcggcgg tacggtacgc accaacagcg gctgcccgaa catattttcc
    56221 agttgcttaa tgcgctgtga gacggctgat tgtgtaatgc acagcttttg tgccgcgcgc
    56281 tcaaatcctc gttcacgtat caccgcatcc agtgcctgta atgttctgta gtccgggcgt
    56341 ttcattgctg ttgtctgctc ctggtaaatg ctttttcctg cactatgaca caattttgtg
    56401 tcaggttgca tataccgcag ccgtcggcgg gtaacatttg tatcaatggt taaacgcgca
    56461 gcttaaaaga tcagggattt gcgctattgc atttcgctta gcaacttcgc gccctggtta
    56521 tgttgaatgg cgtggcgtta ttgcctctgt tcgcaccgtg gagcggcata cccagaccgt
    56581 tgtatgcttt atttgcgggg cgaaagggga tgcccgccat tgcgcgatat tttatggatg
    56641 agttaaccac gcggcttgct aacggggtct gaatcgcttt ttttgtatat aatgcgtgtg
    56701 aaatttcata ccacaggcga aacgatcatg acgcaggatg aattgaaaaa agcagtagga
    56761 tgggcggcac ttcagtatgt tcagcccggc accattgttg gtgtaggtac aggttccacc
    56821 gccgcacact ttattgacgc gctcggtaca atgaaaggcc agattgaagg ggccgtttcc
    56881 agttcagatg cttccactga aaaactgaaa agcctcggca ttcacgtttt tgatctcaac
    56941 gaagtcgaca gccttggcat ctacgttgat ggcgcagatg aaatcaacgg ccacatgcaa
    57001 atgatcaaag gcggcggcgc ggcgctgacc cgtgaaaaaa tcattgcttc ggttgcagaa
    57061 aaatttatct gtattgcaga cgcttccaag caggttgata ttctgggtaa attcccgctg
    57121 ccagtagaag ttatcccgat ggcacgtagt gcagtggcgc gccagctggt gaaactgggc
    57181 ggtcgtccgg aataccgtca gggcgtagtg accgataacg gcaacgtgat cctcgacgtc
    57241 cacggcatgg aaatccttga cccgatagcg atggaaaacg ccataaatgc gattcctggc
    57301 gtggtgactg ttggcttgtt tgctaaccgt ggcgcggacg ttgcgctgat tggcacacct
    57361 gacggtgtca aaaccattgt gaaatgatct gacgggggaa cctcccccgt taaaaaaatt
    57421 ctcttcatta aatttggtga catgtgtcac gtttttacct ggatattgtc gattgctcta
    57481 aataaatcct ctaaaccagc atattcaacc aagaaatacc tttgcgtgat atttcctcaa
    57541 catcgcgacg caaacgttca tattgccgca atattatttt ttgatatgtt gaaaggcgga
    57601 tgcaaatccg cacacaacat ttcaaagaca ggattgggta aatggcaaag gtatcgctgg
    57661 agaaagacaa gattaagttt ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc
    57721 ttcgtgcagc tggttacacc aacatcgaat ttcacaaagg cgcgctggat gatgaacaat
    57781 taaaagaatc catccgcgat gcccacttca tcggcctgcg atcccgtacc catctgactg
    57841 aagacgtgat caacgccgca gaaaagctgg tcgctattgg ctgtttctgt ataggaacaa
    57901 accaggttga tctggatgcg gcggcaaagc gcgggatccc ggtatttaac gcaccgttct
    57961 caaatacgcg ctctgttgcg gagctggtga ttggcgaact gctgctgcta ttgcgcggcg
    58021 tgccggaagc caatgctaaa gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt
    58081 ttgaagcgcg cggcaaaaag ctgggtatca tcggctacgg tcatattggt acgcaattgg
    58141 gcattctggc tgaatcgctg ggaatgtatg tttactttta tgatattgaa aacaaactgc
    58201 cgctgggcaa cgccactcag gtacagcatc tttctgacct gctgaatatg agcgatgtgg
    58261 tgagtctgca tgtaccagag aatccgtcca ccaaaaatat gatgggcgcg aaagagattt
    58321 cgctaatgaa gcccggctcg ctgctgatta atgcctcgcg cggtactgtg gtggatattc
    58381 cggcgctgtg tgatgcgctg gcgagcaaac atctggcggg ggcggcaatc gacgtattcc
    58441 cgacggaacc ggcgaccaat agcgatccat ttacctctcc actgtgtgaa ttcgacaacg
    58501 tccttctgac gccacacatt ggcggttcga ctcaggaagc gcaggagaat atcggcctgg
    58561 aagttgcggg taaattgatc aagtattctg acaatggctc aacgctctct gcggtgaact
    58621 tcccggaagt ctcgctgcca ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc
    58681 gtccgggcgt gctaactgcg ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg
    58741 cgcaatatct acaaacttcc gcccagatgg gttatgtagt tattgatatt gaagctgacg
    58801 aagacgttgc cgaaaaagcg ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc
    58861 gtctgctgta ctaattcccc ttctctgaaa attaacgggc aggtcactga cttgcccgtt
    58921 tttttatccc ttctccacac cgttgccgcg ttgcgcattc tactttgcga gtctgctcca
    58981 gaaaataaat caaagtcaga tcaatgcgtt gtgtttattt aatggggatt tacgatggcg
    59041 ggacagcatg gggctgtaac ggtgaagccc tcaccgaagc gagggcttaa aggagaaggg
    59101 ttatgatgcg acttgtcatc atactgattg tactgttact gataagtttc ggagcttatt
    59161 aacagtcagt ctcaggggag gagtcatcct cccttaccct tactcactaa attaggtcaa
    59221 agaatcaacg atgtcaatca gggcgatgcg gggtgcatcg ccctgaaccg aactttttga
    59281 tagccacttc atcgcccacg tttactgccg taattaatcc cttattcctg caatgtatgt
    59341 ctgccttaca tgacgtttta cgagtgcgct cgaagaaaag atttaaaggt agatcaatgc
    59401 gttgtggtta tttaaagaga gtttacgatg gcagggcagc atggggctgt aacggtaaag
    59461 ccctcaccga agcgagggct tgaaggagaa gggttatgat gcgacttgtc atcatactga
    59521 ttgtactgtt actgataagt ttgccagctt attaacagtc agtctcaggg gaggagtcat
    59581 cctcccttac ccttactcac taaattaggt taaagaatca acgatgtcaa tcagggcgat
    59641 gcaggtgtat cgcccttacc actcccagac tttcgacggt gtaaccaccg caggaagagg
    59701 gatatcccac tcttcaacgg ggagtttttc caccaactga caatcatgcg cataacccac
    59761 cggttgcgtt ttatagtgct gccagttttg taaggtccga tcataaaaac caccgcccat
    59821 tcccaggcgc tgaccgtact catcaaaggc gaccagcggt gtgatcagca cgtctaatcg
    59881 ggaaaggggg agcacgtcac gcacatccag ttttggctca tggattttca acctgttcat
    59941 caccagttcg ctttgcggat ggtaattcag gaacagcaaa ttaccggcac taaagggatg
    60001 caaaactgga agatatacgc gcttaccggc gcgccagagt tgttctataa gcggctgggt
    60061 gtcgagttca ccatcaaaag agaggaatac agcgaccgta tgcgccatca ccaccggggg
    60121 ataagtcatc atccgggtag cggcttgttg acccatttcc tgctgttgtt ccggcgttaa
    60181 cgcacgacga cgttgccgaa tcattttgcg gatttcttgt cgggataatg tcagtgggag
    60241 ttctggtagt tgcgtcatgg ctggtgccag ataagaaggg aatctccgag atgccgccgc
    60301 aggctgtaac ccttgaaccc ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc
    60361 tcggacggac cgagcatgct caccaaccgc ggagcgccac attcttgtgg tatgaaatat
    60421 cggctcaggg gactggcccg cttgcgaaca tctcagagaa attttgtctt cacggttact
    60481 ctaccacagt aaaccgaaaa gtgtcattca aagttttggt tagttttttc ggtgatgcga
    60541 ccttgttcaa gtaacgcttg ttctatggtc tgctgcagca tccgaatacg ctgttccata
    60601 cttgccgcgt agtcacgagt cttcgctttt tcttgcgcta actcatagct gatattcaat
    60661 gcggcaatga agaccaactg ttcagtattt gtgactctag tgcgttcttt cagatcttgc
    60721 aaccgttggt tcagatcgtc cgctgcctga ttcaacgcat ccctttggtc aggcgggcag
    60781 ttcacacgca gtgaacggcc aaaaatttgg atatcgacgg gttgtgcaga catgccacct
    60841 tcctgctgat tgactgcgct gccttcgtct ttagacccat ggtctgcgaa ggggcgacac
    60901 tatagctacc ctgatgagaa gagacaagcc cttttctggt ccaccagggc ccaaagtggt
    60961 agcatatcat gaatattcct ccctttgacg acgaatgctt atgtctatac agaacgaaat
    61021 gcctggttac aacgaaatga accagtatct gaaccaacaa gggacgggtc tgacccctgc
    61081 tgagatgcat ggtttaatca gtgggatgat atgtggcggt aacgatgaca gctcatggct
    61141 accgctactt cacgacctga cgaacgaagg catggctttc ggtcatgagc tggcacaggc
    61201 attgcgtaaa atgcactctg ccaccagcga tgccctgcag gatgacggct tcctttttca
    61261 gctttatctg cctgatggcg atgatgtcag cgttttcgat cgggctgatg cattggcagg
    61321 ttgggtcaat cacttcctgc ttggtcttgg tgttacgcaa ccgaagctgg ataaagtgac
    61381 cggcgaaacc ggtgaagcta tcgacgatct gcgtaacatt gcgcaactgg gttacgacga
    61441 agacgaagat caggaagagc ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt
    61501 tgccgcgctg ttatgccacg acacctttac tcatccgcaa ccgaccgcgc cagaagtaca
    61561 aaaaccgact ctacactaaa aacaaaaaac gtaaggagag tgttatgagt gagatatccc
    61621 ggcaagagtt tcagcgtcgc cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg
    61681 cgctgatttt tgctgcacca gaagtaacac gtagcgccga cagcgaatac ccctatcgtc
    61741 agaacagtga cttctggtac ttcaccggct ttaacgaacc ggaagcggtg ttggtgctga
    61801 ttaaaagcga tgacactcat aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg
    61861 cggagatctg gtttggccgt cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg
    61921 accgcgcact ggcattcagc gaaattaatc agcaacttta tcaactactt aatggtctgg
    61981 atgtggttta ccatgctcag ggcgaatatg catatgctga tgaaatcgtg aacagtgcgc
    62041 tggaaaaact gcgtaaaggt tcgcggcaaa atctcaccgc accggcaacg atgatcgact
    62101 ggcgtcctgt tgttcatgaa atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc
    62161 gccgcgcggg agaaatcacc gccatggcac atacacgggc gatggaaaaa tgccgtccgg
    62221 gaatgttcga gtaccatctg gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc
    62281 gctatccgtc ctataacacc attgtaggca gcggtgaaaa cggctgcatt ttacactaca
    62341 ccgaaaacga gtgtgaaatg cgcgacggcg acctggtgtt gattgacgca ggctgcgaat
    62401 acaaaggtta cgcaggcgat atcacccgta ccttcccggt caacggcaaa ttcacccagg
    62461 cccagcgcga aatctacgac attgtgctgg agtctcttga aaccagcctg cgcctgtatc
    62521 gtccgggaac ctccattctg gaagtcaccg gtgaagtggt gcgcattatg gttagcggcc
    62581 tggtaaaact cggcatcctg aaaggtgata ttgatgaact gatcgctcag aacgcccatc
    62641 gtcctttctt tatgcatggc cttagccact ggttaggact ggatgtccat gacgttggcg
    62701 tttatggtca ggatcgctcg cgcattctgg aaccggggat ggtactgacc gtagagcctg
    62761 gtctgtatat cgcgccggat gcggatgtgc cagaacaata tcgcggtatc ggcattcgta
    62821 ttgaagacga cattgtgatt accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga
    62881 aaaagccgga agaaatcgaa gcgttgatgg ctgctgcgag aaagcaatga gcgtaatcat
    62941 cgtcggtggc ggcatggcgg gcgcgacgct ggcgctggct atttcccggt taagtcacgg
    63001 ggcgctgccg gtacatttga ttgaagcgac tgcgccagag tcacatgctc atccgggctt
    63061 tgatggacga gcgatagcgc tggcggcggg tacctgtcag caactggcgc gcatcggcgt
    63121 ttggcaatct ctggcggatt gcgcaactgc catcaccacc gtgcatgtca gcgatcgagg
    63181 tcacgctgga tttgtcaccc tcgccgcaga agattaccaa ctggcggcgc tgggacaggt
    63241 tgtcgaattg cacaatgtcg ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt
    63301 aacgctgcat tgccctgatc gcgtggctaa cgttgcccgt actcagagtc acgttgaagt
    63361 gacgctggag ggtggcgaga cgctgacggg ccgcgtgctg gtagccgctg atggcaccca
    63421 ttcagcgtta gccactgtgt gcggcgttga ctggcagcag gagccttacg aacaactggc
    63481 tgtgattgcc aacgttgcga cttccgttgc gcatgaaggg cgcgcttttg aacgctttac
    63541 gcaacatggc ccgctggcga tgttgccgat gtctgacgga cgctgttcgc tggtctggtg
    63601 tcatccactg gaacggcgcg aagaggtgct gtcgtggagc gacgagaagt tttgccgtga
    63661 actccagtcg gcctttggct ggcgacttgg gaaaattacc cacgctggta aacgcagtgc
    63721 ttatccgctg gcgttaaccc acgccgccag atctattacc catcgtaccg tgctggtggg
    63781 caatgcggcg caaaccctgc acccgattgc cgggcaaggg ttcaacctcg gtatgcgaga
    63841 tgtcatgagt cttgcggaaa ccctgactca ggcgcaggag cgcggagaag acatgggtga
    63901 ttacggcgta ttgtgtcgtt atcagcagcg tcgacagagc gatcgcgaag caaccattgg
    63961 cgtcacggac agccttgtac atctttttgc caaccgttgg acgccactgg ttgtcgggcg
    64021 caacatcggg ctgatgacga tggaattatt caccccggca cgcgatgtgc tggcgcagcg
    64081 caccctcggt tgggtggcgc gttgaaggcg gttattaagg aataaacaat gcaaagtgtt
    64141 gatgtagcca ttgttggcgg cggcatggtg gggctggcgg ttgcctgcgg cttacagggt
    64201 agcggcttac gcgttgccgt actggagcag cgcgtaccgg aacctctggc ggcggatgca
    64261 ccaccacaac tgcgcgtttc ggctatcaat gccgccagcg aaaaattact cacccgtctt
    64321 ggcgtctggc aggacattct ctctcgccga gccagctgtt atcacggtat ggaagtgtgg
    64381 gacaaagaca gttttggtca catctcgttt gacgatcaaa gcatgggcta tagccatctt
    64441 gggcacatcg ttgaaaactc agtgattcac tacgcgctgt ggaacaaagc gcagcagtcg
    64501 tcagatatca ctcttttggc ccccgcagaa ttacagcagg tcgcctgggg agaaaatgaa
    64561 accttcctga cgctgaaaga tggcagtatg ttaacggcgc gtctggtgat tggcgcggac
    64621 ggcgctaatt cctggttgcg caacaaagcc gatattccgc tgactttctg ggattatcag
    64681 catcacgcgc tggtagcgac catacgcacg gaagaaccgc atgatgcggt ggcgcggcag
    64741 gttttccatg gcgaaggcat tctggccttt ttaccgctta gcgatccgca tctttgctcg
    64801 attgtctggt cactgtcgcc agaggaagcg cagcggatgc agcaggcaag tgaagacgaa
    64861 tttaatcgcg cgttaaatat cgcttttgat aatcgcctgg gcttatgcaa ggttgagagc
    64921 gcgcgtcagg tgttcccact gacggggcgt tatgcgcgcc agtttgccgc gcaccgtctg
    64981 gcgctggtgg gcgacgccgc gcataccatt cacccgctgg cggggcaggg ggtaaatctt
    65041 ggctttatgg atgctgcaga gctgattgct gaactgaaac ggttgcatcg tcagggtaaa
    65101 gacatcgggc agtacattta tctgcgtcgc tatgagcgta gccgcaagca cagtgcggcg
    65161 ctgatgctgg ctggtatgca gggattccgc gatctgtttt ctggtaccaa tccggtgaaa
    65221 aaactgctgc gtgatattgg tctgaaactg gccgacacgc ttcctggcgt taaaccacaa
    65281 cttatccgcc aggcaatggg attaaacgat ttgcctgaat ggctgcgtta aaaatttctc
    65341 ttctgctgtt tatttgatac ccatcacact ttcatctccc ggttttttcg ccgggagatt
    65401 ttcctcattt gaaataaact aatttcacct ccgttttcgc attatatttt ctaatgtcac
    65461 gaatttttga tttagtgttt tttgacattt tttcagctct taatattgcc ttattcaaat
    65521 taactttctc atcacatcat ctttatatag aaacttgtgt attttttggt tttttattct
    65581 gttgcgattt tggcgttttt taaccataag ctaatgtgat gatcaatttt accttatggt
    65641 taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg gggaccggga
    65701 gtggctccga tgctgggttt cgtggtgata atttcaccat gaaaaagttg tcagccccgc
    65761 ttattcaatg aggacaagat ggcacaacag actcctttgt acgaacaaca cacgctttgc
    65821 ggcgctcgca tggtggattt ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc
    65881 gacgaacatc atgaggtacg taccgatgcc ggaatgtttg atgtgtcaca tatgaccatc
    65941 gtcgatcttc gcggcagccg cacccgggag tttctgcgtt atctgctggc gaacgatgtg
    66001 gcgaagctca ccaaaagcgg caaagccctt tactcgggga tgttgaatgc ctctggcggt
    66061 gtgatagatg acctcatcgt ctactacttt actgaagatt tcttccgcct cgttgttaac
    66121 tccgccaccc gcgaaaaaga cctctcctgg attacccaac acgctgaacc tttcggcatc
    66181 gaaattaccg ttcgtgatga cctttccatg atcgccgtac aagggccgaa tgcgcaggca
    66241 aaagctgcca cactgtttaa tgatgctcag cgtcaggcgg tggaagggat gaaaccgttc
    66301 tttggcgtgc aggcgggcga tctgtttatt gccaccactg gttataccgg tgaagcgggc
    66361 tatgaaattg cgctgcccaa tgaaaaagcg gccgatttct ggcgtgcgct ggtggaaggg
    66421 ggcgttaagc catgcggtct gggcgcgcgt gacacgctgc gtctggaagc gggcatgaat
    66481 ctttatggtc aggagatgga cgaaaccatc tctccgttag ccgccaacat gggctggact
    66541 atcgcctggg aaccggcaga tcgtgacttc atcggtcgtg aagccctgga agtgcagcgt
    66601 gagcatggca cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat
    66661 gaactgccgg tacgttttac cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc
    66721 gggactttct ccccgacgct gggttacagc attgcgctgg cacgcgtgcc gaaaggtatt
    66781 ggcgaaacgg cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt gacaaaacct
    66841 gtttttgtgc gtaacggcaa agccgtcgcg tgatttactt ttttggagat tgattgatga
    66901 gcaacgtacc agcagaactg aaatacagca aagaacacga atggctgcgt aaagaagccg
    66961 acggcactta caccgttggt attaccgaac atgctcagga gctgttaggc gatatggtgt
    67021 ttgttgacct gccggaagtg ggcgcaacgg ttagcgcggg cgatgactgc gcggttgccg
    67081 aatcagtaaa agcggcgtca gacatttatg cgccagtaag cggtgaaatc gtggcggtaa
    67141 acgacgcact gagcgactcc ccggaactgg tgaacagcga accgtatgca ggtggctgga
    67201 tctttaaaat caaagccagc gatgaaagcg aactggaatc actgctggat gcgaccgcat
    67261 acgaagcatt gttagaagac gagtaacggc tttattcctc ttctgcggga gaggatcagg
    67321 gtgaggaaat ttacacctca ccctcactct cttcgcaagg agagaggttc acaattcact
    67381 gcacgtttca ggaaccatcg ctcatgacac agacgttaag ccagcttgaa aatagcggcg
    67441 cttttattga acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg
    67501 ttggcgcaca atcgttaaac gcgctgaccg gccagattgt accgaaagat attcagcttg
    67561 cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc cgcgctggcg gaactcaagg
    67621 ctattgccag tcgcaataaa cgcttcacgt cttacatcgg catgggttac accgccgtgc
    67681 agttaccgcc ggttatcctg cgtaacatgc tggaaaatcc gggctggtat accgcgtata
    67741 ctccgtatca acctgaagtc tcgcagggcc gccttgaggc actgctcaat ttccagcagg
    67801 taacgctgga tttaaccggg ctggatatgg cctctgcttc tcttcttgac gaagccaccg
    67861 ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct
    67921 tcttcgtggc ttccgatgtg catccgcaaa cgctggatgt ggtccgtact cgtgccgaaa
    67981 cctttggttt tgaagtgatt gtcgatgatg cgcaaaaagt gctcgaccat caggacatct
    68041 tcggcgtgct gttacagcag gttggcacta ccggtgaaat tcacgactac actgcgctta
    68101 tcagcgaact gaaatcacgc aaaattgtgg tcagcgttgc cgctgatatt atggcgctgg
    68161 tgctgttaac tgcgccgggt aaacagggcg cggatattgt ttttggttcg gcgcaacgct
    68221 ttggcgtacc gatgggttac ggtggcccac acgcggcatt ctttgcggcg aaagacgaat
    68281 acaaacgctc aatgccaggc cgtattatcg gtgtatcgaa agatgcagct ggcaataccg
    68341 cgctgcgcat ggcgatgcag actcgcgagc aacatattcg tcgtgagaaa gcgaactcca
    68401 acatttgtac ttctcaggtg ctgctggcaa acatcgccag cctgtatgcc gtttatcacg
    68461 gcccgattgg cctgaaacgt atcgctaacc gcattcaccg tctgaccgat atcctggcgg
    68521 caggcctgca acaaaaaggt ctgaagctgc gccatgcgca ctatttcgac actctgtgtg
    68581 tggaagtggc cgacaaggcg ggcgtactgg cgcgtgccga agcggctgaa atcaacctgc
    68641 gtagcgatat cctgaacgcg gttgggatca cccttgatga aaccaccacg cgcgaaaacg
    68701 tgatgcagct tttcagcgta ctactggggg ataaccacgg gctggacatc gacacgctgg
    68761 acaaagacgt ggctcacgac agccgctcta tccagcctgc gatgctgcgc gacgacgaaa
    68821 tcctcaccca tccggtgttt aatcgctacc acagcgaaac cgaaatgatg cgctatatgc
    68881 actcgctgga gcgtaaagat ctggcgctga atcaggcgat gatcccgctg ggttcctgca
    68941 ccatgaaact gaacgccgcc gccgagatga tcccaatcac ctggccggaa tttgccgaac
    69001 tgcacccgtt ctgcccgccg gagcaggccg aaggttatca gcagatgatc gcgcagctgg
    69061 ctgactggct ggtgaaactg accggttacg acgccgtttg tatgcagccg aactctggcg
    69121 cacagggcga atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag
    69181 ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg cactaacccc gcttctgcac
    69241 atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa aaacggaaat atcgatctga
    69301 ctgatctgcg cgcgaaagcc gaacaggcag gtgataatct ctcctgcatc atggtgacct
    69361 atccttccac ccacggtgtg tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc
    69421 agttcggcgg tcaggtttac cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct
    69481 cgccgggctt tattggcgca gacgtttcac accttaacct gcataaaact ttctgcattc
    69541 cgcacggcgg tggtggtccg ggtatgggac cgatcggcgt gaaagcgcat ctggctccgt
    69601 ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag ggcgcggttt
    69661 ctgcggcacc gttcggtagc gcctctatcc tgccaatcag ctggatgtac atccgcatga
    69721 tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat cctcaacgcc aactatattg
    69781 ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg tcgtgacggt cgcgtggcgc
    69841 acgaatgtat tctcgatatt cgtccgttga aagaagaaac cggcatcagc gagctggata
    69901 ttgccaagcg cctgatcgac tacggtttcc acgcgccgac gatgtcgttc ccggtggcag
    69961 gtacgctgat ggttgaaccg actgaatctg aaagcaaagt ggaactggat cgctttatcg
    70021 acgcgatgct ggctatccgc gcagaaattg accaggtgaa agccggtgtc tggccgctgg
    70081 aagataaccc gctggtgaac gcgccgcaca ttcagagtga actggtcgcc gagtgggcgc
    70141 atccgtacag ccgtgaagtt gcggtattcc cggcaggcgt ggcagacaaa tactggccga
    70201 cagtgaaacg tttggatgat gtttacggcg accgtaacct gttctgctcc tgcgtaccga
    70261 ttagcgaata ccagtaattc actgattcga ctatcttcta aaggcgcttc ggcgcctttt
    70321 tagattgtta ataaagtgcg ctttgtgtgt ttcggctgag gcgtaatata aaatattgcg
    70381 agctcaatat attaaaagag tgccaggcct gatgagcgta gcgcatcagg cacatcggct
    70441 aattacagat tctcgccgtt gctggcaatc acttctttgt accagttaaa gctcttctta
    70501 cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat tcacatagat aaagccgtag
    70561 cgtttgctgt actgcccggt agtgaacgac acgcagtcga tgcagcccca cggtgtgtag
    70621 cccatcagat ccacgccatc gtaagttact gcttttttca tctcttcaat atgggcgcgc
    70681 aggtagtcaa tgcggtagtc gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg
    70741 ccaaaaccgt tttcgacaat aaacagcggc ttctggtaac gctcatacag ttcgcaaagt
    70801 gcgtaacgca gacccaccgg atcaatctgc cagccccagt cagatgcttt aacatacggg
    70861 tttggtacgc tgccttcaaa accagagatc gcatcgccgg tgccgccttc ggccttcact
    70921 gcgttggtca tgtaatagct gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg
    70981 agatcgctat cttccatttt gatgttaaat ccgcgacgct cccactcgtt caacacatac
    71041 gacgggtaat agccgcgtag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc
    71101 tgagcgaaca tcacatcgtc cgggttacat gagtagggat agagcggcac catcgccagc
    71161 atacagccga ctttcatctc caggttaatg cgacgcgcgg ctttcactgc cagggcgctg
    71221 gcgacaaact ggtgatgcag cacctgatac atcgtctctt ccgggttttc atgctcggta
    71281 tacaccacgc cggagcagca gtaaccgaac agcggtgcac gccagttacg ctggttgtta
    71341 atttcgttga aggtcatcca gtatttgact ttgtgcttat agcgttcaaa taccacttcc
    71401 gcgaaacgta caaagaaatc aaccacttta cggttggtcc agctaccgta ttgctgcacc
    71461 agatgcagcg gcatttcaaa gtgggagagg gtgatcaccg gttcgatgtt gtatttcagc
    71521 agttcatcga acagagagtc gtagaacttc agcccttctt cgtttggctg agcttcatcg
    71581 ccttttggaa aaatgcgcgt ccaggcaatg gatgtacgaa aacatttgaa gcccatttcg
    71641 gaaaatagct tgatgtcttc cttatagtga ccataaaaat caacggcttc atggtttgga
    71701 tagtatttgc ctggcaagac ttctttggtt atttcgcgcg gcacgccgtg tgcgccaccg
    71761 gtcagaacgt cacaaatgct cggccctttt ccgcctttgt tccagccgcc ttcgacctga
    71821 tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta aggtgagttt tttcactatc
    71881 ataggctcca gatgagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac
    71941 tttttttcaa cgtttccagg atgacattct ggctgggcgt aaaaccatca ccatccgcga
    72001 cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt gtcggacgtt ttgaagatga
    72061 cggttatttt tgcacgattg aagtcaccgc aacctcaacc gtaacgctgg atacgctgac
    72121 ggaaaaacat gcagagcagg aaaatatgac cctgactgaa ctgaaaaagg tcattgccga
    72181 catctatccc ggccagacac agttttatgt gattgaattt aaatgccttt aaattgaact
    72241 cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt tttaggagat tagtctcaaa
    72301 tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg
    72361 tatacatgtg cgtgtacgtt tctccggagt aagttatggt tcagaagccc ctcattaagc
    72421 agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt gggttggtgt
    72481 ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca
    72541 cagcgataac cagctacagc ctctatggcg gcagtatgat cctgctgttc ctcgcttcga
    72601 cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccatt
    72661 gcgctattta cctgttgatt gccggaacct acacgccgtt tttgctggtg gggctggatt
    72721 ctccgttagc gcgcgggttg atgattgtta tctggagcct ggcattgctg gggattctgt
    72781 tcaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacttat ctggcgatgg
    72841 gctggctgtc gctggtggta atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta
    72901 ccttactggc ggtaggcggc gtggtttatt cgctcggggt gattttctac gtctgcaaac
    72961 gcattccata caaccatgcc atctggcacg gcttcgtgct cggcggtagt gtgtgccact
    73021 ttctggcgat ctatttgtat attgggcagg cgtaatgggt atgcctttgt aggcctgata
    73081 agacgcgcca gcgtcgcatc aggcgataaa tgcctgatgc gacacagaga tttactcttc
    73141 gagcgaatac ggcagtggct cgatacacaa tgtattcgca tcgtcgcgca cgcggaagat
    73201 gctgtccggt tccatatcgt tattcatcac tacctgcacc acgacctgac catcttccag
    73261 ttttaccgca gccagcaccg taccagtacg acgccagttc tcgcccattt tcagctctaa
    73321 gtcttcacca gcttccggca gtcggctggc actacctgtc agcaaccaga gcgcgcgttt
    73381 gttggcacca cggaattttg ctcgcgccac catctcttgt ccggtatagc agcctttctt
    73441 aaagctgata ccgcccagcg cctggaggtt ggtcgcctgt gggataaact gcccgctgtt
    73501 cgcggcatca atcaccggga aacccgcttc aatgtttaat gccagccact gttggctatt
    73561 gttcagttcc gcttcaccgc gcagtttatc ggtcagcata ttggcagtag cttcatcggt
    73621 tacgatcagg aaacgttctg ccgggtgttc aaaccatagc aaagtggtcg cgccttcttt
    73681 aactacctgt ttttctttcg aaggcagttc actaaagata tttgccagcg cggcgcgcgc
    73741 ctgaaaaccg gcaacaccaa gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga
    73801 gaacaccgca tattttttca gttcagccag ctgcggttca cgcacgctgc gccgttcaat
    73861 ccatgcaaag ccatcgccgt cgcggaacag acgtaaattg ctccacattt tacctttggc
    73921 gtcgcaatgg gcggcgagca ggtgctgatc atctgtcatc tggctgacat ctgctgtcac
    73981 ctggccctgc aaatattttt cgctgtccgc gccagtaatg gtggcaagcg cccagtcatc
    74041 aagcgtcatc agcgtcagtg gcaaacgggc agaagccgta ggctgacggg gaggaaaagg
    74101 tgtaaaagcc ataatagagt cctgagttgc ttaacggcgt attaataact aatggtaaaa
    74161 gagctatgag gcaatgcaag tgctttcact acgcgatctg tgccttctgc gaggcgactt
    74221 cccaaaaaat gaagccctta aattgttgtg aattaatttc gcgatcgcga ttgtgtttac
    74281 tgatattccc gtaaaagaag tcgaaaaaca cgttacaata gccgggtact ggattttcgt
    74341 gagacaggaa gaagatggac attaacaaca aagcccgcat tcattgggca tgccgccgtg
    74401 gtatgcgcga actcgatatt tcaatcatgc cgtttttcga acatgagtat gacagcttaa
    74461 gcgatgacga aaaacgcatc tttattcgtc tgctggaatg tgacgatccc gatctgttta
    74521 actggctgat gaatcacggt aaaccggccg atgcagaact ggaaatgatg gtccgactca
    74581 tccagacacg gaaccgggaa cgtggtcctg tggcaatctg atttgcgcgt ctcctggcgc
    74641 gcacagtggc tttccttgct gattcatggg ctggttgccg ctgttatttt actcatgccc
    74701 tggccgctca gttacacccc gttatggatg gtgttacttt cgctggtggt gtttgattgc
    74761 gttcgcagcc agcggcgtat taatgctcgc cagggggaaa ttcgcttgtt gatggacggg
    74821 cgtttgcgtt ggcaagggca ggagtggagc atcgtcaaag cgccgtggat gattaagagc
    74881 ggcatgatgc tgcgtttacg ttctgatagc ggtaaacggc aacatttatg gctggcagcc
    74941 gacagcatgg acgaagccga atggcgggat ttacggcgga ttttgttgca acaagagacg
    75001 caaagataaa gatgacggca aagcatatgc gctaagccgt cagtgatatc aggcgtaatg
    75061 ctctgccatt tcgttgagga tttgctcgca ccagctctga atacgctcgt cgctaaggtc
    75121 atactggtta gtttcgtcca gcgccagccc cacgaacagt tgcccgtcag caatcaccgg
    75181 tttcgggctg gtaaattcat acccttccgt tggccagtag ccgacgaact tcacgccttt
    75241 ggtcgagagt ttgtcatgca gcataccgag cgcatcgagg aaccactcgc cgtatcccag
    75301 ttgatcgcca agcccataca gcgcaacaat tttaccttcg aggttcaggt cgtcgagctg
    75361 atcccagacg gcttcccagt cttcctggat ttcgccaaaa tcccaggtcg ggatacccag
    75421 aatgagcaca tcgtactgct ccattaattt cggggagtcg tccttgaggt tatgtaaggt
    75481 caccagttct gggccgataa tatcgcggat tttttctgcc gccatttcgg tgtaacaggt
    75541 gctggaaccg taaaaaagac ccatattcat aacgtaagct tctcgattct gctgtggctt
    75601 ttgcggctag tgtatcagaa tcgctttagt cacggcacaa taaggcataa tggatcaaat
    75661 gagtgaccat aaggggcgca agtgaaacag gatctggcac gcatcgagca gtttcttgat
    75721 gctctgtggc tggaaaaaaa tctggctgaa aatacgttga acgcttaccg tcgcgatctg
    75781 tcaatgatgg tggagtggtt gcatcaccgc gggttgacgc tggcgacggc gcaaagtgac
    75841 gatttacagg cattactggc agaacggctg gagggcgggt ataaagccac cagctcagcg
    75901 cgtttgctga gtgcagtgcg ccgattgttc cagtatcttt atcgcgaaaa gtttcgtgaa
    75961 gatgatccca gtgcgcatct cgcttcaccg aaattgcccc agcgtttgcc aaaagattta
    76021 agcgaagcgc aggtcgaacg tttattacag gcaccattaa ttgatcagcc actggagcta
    76081 cgcgataaag ctatgcttga agtgttgtat gctaccgggc tgcgtgtctc tgaactggtc
    76141 gggctgacaa tgagtgatat cagcctgcgt cagggcgtag tgcgggtcat tggtaaaggc
    76201 aacaaagagc gcctggtgcc gttaggtgaa gaggcggttt actggctgga aacctatctg
    76261 gaacatgggc gtccgtggct actgaatggt gtgtcaattg acgtgttgtt tcccagccag
    76321 cgtgcgcagc agatgacgcg acagaccttc tggcaccgca ttaaacatta tgccgtgctg
    76381 gcgggtatcg acagcgaaaa gctgtcaccg catgtgttgc gtcacgcttt tgccactcac
    76441 ttattaaatc atggtgcgga tttacgcgtg gtgcagatgc tactgggcca cagcgatctc
    76501 tccaccacgc aaatttatac gcatgtcgct accgagcgtc tgcggcaact tcatcaacag
    76561 catcacccgc gggcgtgatg tctgaaaaga acgggaagat ttatgaagaa aggttttatg
    76621 ttgtttactt tgttagcggc gttttcaggc ttcgctcagg ctgatgacgc ggcaattcaa
    76681 caaacattag ccaaaatggg catcaaaagc agcgatattc agcccgcgcc cgtagctggc
    76741 atgaagactg tgctgactaa cagcggtgtg ttgtacatca ccgatgatgg taaacatatc
    76801 attcaggggc caatgtatga cgttagcggc acggctccgg tcaatgtcac caataagatg
    76861 ctgttaaagc agttgaatgc gcttgaaaaa gagatgatcg tttataaagc accgcaggaa
    76921 aaacacgtca tcaccgtgtt tactgatatt acctgtggtt actgccacaa actgcatgaa
    76981 caaatggcag actataacgc gctggggatc accgtgcgtt atcttgcttt cccacgccag
    77041 gggctggaca gcgatgctga gaaagaaatg aaagctatct ggtgtgcgaa agataaaaac
    77101 aaagcgtttg atgatgtgat ggcaggtaaa agcgtcgcac cagccagctg cgacgtggat
    77161 attgccgacc attacgcact tggcgtccag cttggcgtta gcggtactcc ggcagttgtg
    77221 ctgagcaatg gcacacttgt tccgggttac cagccgccga aagagatgaa agaattcctc
    77281 gacgaacacc aaaaaatgac cagcggtaaa taattcgcgt gaaacaacag atacaacttc
    77341 gtcgccgtga agtcgatgaa acggcagact tgcccgcgga attgcctccc ttgctgcgcc
    77401 gtttatacgc cagccgggga gtacgcagtg cgcaagaact ggaacgcagt gttaaaggta
    77461 tgctgccctg gcagcaactg agcggcgtcg aaaaggccgt tgagatcctt tacaacgctt
    77521 ttcgcgaagg aacgcggatt attgtggtcg gtgatttcga cgccgacggt gccaccagca
    77581 cggctctaag cgtgctggcg atgcgctcgc ttggttgcag caatatcgac tacctggtac
    77641 caaaccgttt cgaagacggt tacggcttaa gcccggaagt ggtcgatcag gcccatgccc
    77701 gtggcgcgca gttaattgtc acggtggata acggtatttc ctcccatgcg ggagttgagc
    77761 acgctcgctc gttgggcatc ccggttattg ttaccgatca ccatttgccg ggcgacacat
    77821 tacccgcagc ggaagcgatc attaacccta acttgcgcga ctgtaatttc ccgtcgaaat
    77881 cactggcagg cgtgggtgtg gcgttttatc tgatgctggc gctgcgcacc tttttgcgcg
    77941 atcagggctg gtttgatgag cgcggcatcg caattcctaa cctggcagaa ctgctggatc
    78001 tggtcgcgct ggggacagtg gcggacgtcg tgccgctgga cgctaataat cgcattctga
    78061 cctggcaggg gatgagtcgc atccgtgccg gaaagtgccg tccagggatt aaagcgctgc
    78121 tggaagtggc aaaccgtgat gcacaaaaac tcgccgccag cgatttaggt tttgcgctgg
    78181 ggccacgtct caatgctgcc gggcgactgg acgatatgtc cgtcggtgtg gcgctgttgc
    78241 tgtgcgacaa catcggcgaa gcgcgcgtgc tggcaaatga actcgatgcg ctaaatcaga
    78301 cgcgaaaaga gatcgaacaa ggaatgcaga ttgaagcctt gaccctgtgc gagaaactgg
    78361 agcgaagtcg cgacacgcta cccggcgggc tggcaatgta tcaccccgaa tggcatcagg
    78421 gcgttgtcgg tattctggct tcgcgcatca aagagcgttt tcaccgtccg gttatcgcct
    78481 ttgcgccagc aggtgatggt acgctgaaag gttccggtcg ctccattcag gggctgcata
    78541 tgcgtgatgc gctggagcga ttagacacac tctaccccgg tatgatgctg aagtttggcg
    78601 gtcatgcgat ggcggcgggt ttgtcgctgg aagaggataa attcgaactc tttcaacaac
    78661 ggtttggcga gttggttacc gagtggctgg acccttcgct attgcaaggc gaagtggtgt
    78721 cagacggccc gttaagcccg gccgaaatga ccatggaagt ggcgcagctg ctgcgcgatg
    78781 ctggcccgtg ggggcagatg ttcccggagc cgctgtttga tggtcatttc cgtctgctgc
    78841 aacagcggct ggtgggcgaa cgtcatttga aagtcatggt cgaaccggtc ggcggcggtc
    78901 cgctgctgga tggtattgct tttaatgtcg ataccgccct ctggccggat aacggcgtgc
    78961 gcgaagtgca actggcttac aagctcgata tcaacgagtt tcgcggcaac cgcagcctgc
    79021 aaattatcat cgacaatatc tggccaattt agcgtcatct tctctataaa aaagagcgtg
    79081 gattgggtac aatcccgctc ttatcaccgc attttgacta gctcaataaa agaaatcaga
    79141 ccatgtttga aattaatccg gtaaataatc gcattcagga cctcacggaa cgctccgacg
    79201 ttcttagggg gtatctttga ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc
    79261 gagctggaac agccggatgt ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag
    79321 cgttcctccc tcgaagccgt tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat
    79381 gtttctggtc tgctggaact ggctgtagaa gctgacgacg aagaaacctt taacgaagcc
    79441 gttgctgaac tcgacgccct ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc
    79501 tctggcgaat atgacagcgc cgactgctac ctcgatattc aggcggggtc tggcggtacg
    79561 gaagcacagg actgggcgag catgcttgag cgtatgtatt tgcgctgggc agaatcgcgt
    79621 ggtttcaaaa ctgaaatcat cgaagagtcg gaaggtgaag tggcgggtat taaatccgtg
    79681 acgatcaaaa tctccggcga ttacgcttac ggctggctgc gtacagaaac tggcgttcac
    79741 cgcctggtgc gtaagagccc gtttgactcc ggcggtcgtc gccacacgtc gttcagctcc
    79801 gcgtttgttt atccggaagt tgatgatgat attgatatcg aaatcaaccc ggcggatctg
    79861 cgcattgacg tttatcgcgc gtccggcgcg ggcggtcagc acgttaaccg taccgaatct
    79921 gcggtgcgta ttacccacat cccgaccggg atcgtgaccc agtgccagaa cgaccgttcc
    79981 cagcacaaga acaaagacca ggccatgaag cagatgaaag cgaagcttta tgaactggag
    80041 atgcagaaga aaaatgccga gaaacaggcg atggaagata acaaatccga catcggctgg
    80101 ggcagccaga ttcgttctta tgtccttgat gactcccgca ttaaagatct gcgcaccggg
    80161 gttgaaaccc gcaatacgca ggccgtgctg gacggcagcc tggatcaatt tatcgaagca
    80221 agtttgaaag cagggttatg aggaaccaac atgtctgaac aacacgcaca gggcgctgac
    80281 gcggtagtcg atcttaacaa tgaactgaaa acgcgtcgtg agaagctggc gaacctgcgt
    80341 gagcagggaa ttgccttccc gaacgatttc cgtcgcgatc atacctctga ccaattgcac
    80401 gcagaattcg acggtaaaga gaacgaagaa cttgaagcgc tgaacatcga agtcgccgtt
    80461 gctggccgca tgatgacccg ccgtattatg ggtaaagcct cttttgttac cctgcaggac
    80521 gttggcggtc gtattcagct gtacgttgcc cgtgacgatc ttccggaagg cgtttacaac
    80581 gagcagttca aaaaatggga cctcggcgac atcctcggcg cgaaaggtaa actgttcaaa
    80641 accaaaaccg gcgaactgtc tatccactgt accgagttgc gtctgctgac caaagcactg
    80701 cgtccgctgc cggataaatt ccacggcttg caggatcagg aagcgcgcta tcgtcagcgt
    80761 tatctcgatc tcatctccaa cgatgaatcc cgcaacacct ttaaagtgcg ctcgcagatc
    80821 ctctctggta ttcgccagtt catggtgaac cgcggcttta tggaagttga aacgccgatg
    80881 atgcaggtga tccctggcgg tgccgctgcg cgtccgttta tcacccacca taacgcgctg
    80941 gatctcgaca tgtacctgcg tatcgcgccg gaactgtacc tcaagcgtct ggtggttggt
    81001 ggcttcgagc gtgtattcga aatcaaccgt aacttccgta acgaaggtat ttccgtacgt
    81061 cataacccag agttcaccat gatggaactc tatatggctt acgcagatta caaagatctg
    81121 atcgagctga ccgaatcgct gttccgtact ctggcacagg atattctcgg taagacggaa
    81181 gtgacctacg gcgacgtgac gctggatttc ggtaaaccgt tcgaaaaact gaccatgcgt
    81241 gaagcgatta agaaatatcg cccggaaacc gacatggcgg atctggacaa cttcgactct
    81301 gcaaaagcga ttgttgaatc tatcggtatc cacgttgaga agagctgggg tctgggccgt
    81361 atcgttaccg agatcttcga agaagtggca gaagcacatc tgattcagcc gaccttcatt
    81421 actgaatatc cggcagaagt ttctccgctg gcgcgtcgta acgacgttaa cccggaaatc
    81481 acagaccgct ttgagttctt cattggtggt cgtgaaatcg gtaacggctt tagcgagctg
    81541 aatgatgcgg aagatcaggc gcaacgcttc ctggatcagg ttgccgcgaa agatgcaggt
    81601 gacgacgaag cgatgttcta cgacgaagat tacgtcaccg cactggaaca tggcttaccg
    81661 ccgacagcag gtctggggat tggtatcgac cgtatggtaa tgctgttcac caacagccat
    81721 accatccgcg acgttattct gttcccggcg atgcgtccgg tgaaataagc attacgttat
    81781 gcgcataacc ccggcagatg tcggggtttt ttaattgtgc tgcgcgaaag cagacaacaa
    81841 ttttcttgct tcactattgg ctgcctgcat caccatccac ggactgaacg cccacggcgt
    81901 ggcatcaata ccgtgtaata catctgctaa atcacaccat tgataatcca tcacttcatc
    81961 atcgttgatc tgtaacgcac tggtggtgcg tgcggcaaat accggacaca cttcattttc
    82021 cacaatgcca ttcggatcgg tggcgcgata gcgaaagtca ggatagatag attcaggagg
    82081 cgtaatttcc acgccaagct cataacggca acggcggatc accgcgtctt cgttgctttc
    82141 tcccagttgt gggtgcccac aaaccgagtt agtccacacg ccaggccatg cttttttgct
    82201 cagtgcgcgg cgggtaacta ataattgtcc tttggcatta aacagccaac tggagaacgc
    82261 gagatgtaag cgggtgtctg ccgtgtgtgc ggcatacttt tccagcgtac ccgtgggaac
    82321 tccctgtgca ttcaataaaa tgacgtgttc cgtttgcata atttctcaca tgtaattctg
    82381 atcactcatc attatagata attgaagagt gaaatgatta accaagtgag cgtttatcga
    82441 caaccgcccg ttttgagcgg atgccgacag gtaaaaacca tttaaaaaag tagcatcata
    82501 tatttgaagc gactttcaat ggccattgca acacagtcag aaatgacagg aatagagaga
    82561 gtaaaaagca acagcagact gaatatagcg cgcattaaaa aattcatatt attctccatg
    82621 ctcatttttc ttcagcttgc gcatcagaag ccacatacgt actgtcctga cttttaaatc
    82681 tttatcagag acggattcct tcaccaggtc agcatgatgc tgatagccat taaagaagac
    82741 gttcagcaca acggcgctaa gggttgcgag cataatgccg ctatgtagca gcggttgcag
    82801 tacggccggt aacttagaaa agaaatcgtg agagagcgtt ggagtcatgc caacgccgag
    82861 actgatcgcc acaatataga ggttgtaacg gttggtggtg tagttacagc gcgacagaat
    82921 tcgaatccct gtcgccagta ccatgccgaa catcaccaga ccagcgccgc ccagcacaaa
    82981 ttgcggaatg gaggctacca gcaccgccat ttttggcacc atgccaaaca ggattaatat
    83041 aattcccgaa gaaatacaca cccagcggct atgaacgcgc gtcacgctaa ccaggccaac
    83101 gttttgagaa aatgacgtgt gggggaagct gttaaacgtg ccaccaatca ttgtccctac
    83161 gccatcgaca cgcagcccgc gaataatatc gtgcgaagag agtttacgac cgactatttc
    83221 acccagtgcc aggaacatcc ccatcgactc gataaacacg atgattaaca cggcagtcat
    83281 ggtcagaatg gaaacgggat cgaaaatcgg cataccaaac gacatcgggg tgacaatcgc
    83341 aaaccacgag gcatcatgta gcccggataa attgacttca ttcatcatcc acgaaagtaa
    83401 aaagccaaat acaatcccca gtaatacggc gacgttggac ataaatcctt tcgcatagcg
    83461 agtaatgagc aagataaaaa ttaagacggc aaaggagata cctaaataaa cgggattacc
    83521 atattgcgga ttccctttac ctccggcggc ccagtcaata cccacctgaa tgatgctaag
    83581 cccgatagaa gtaataacca caccggtaac cagtggcggg aataaaggca tcaagcgacc
    83641 gataagttgc gctaataatg tggtgataaa acccgcggcg atagtggcac caaatatacc
    83701 cagcaggccg atatccgggt tcatcccaat ggctatcatt ggtgttacag cagcaaaggt
    83761 caccgacata atcaccggca ggcggatccc cataaagcgg ccgataccga tacattgcaa
    83821 taatgtgacg atcccgcagc aaaagagatc cgagctaatg agcatcgcaa tagcttcttt
    83881 tgagaggccc agtcggtcac caatcataag aggaacagcg actgcacctg cgtacatgac
    83941 aaggacgtgt tgcagaccga gtatgatcag ctttcctggt gataatatgc ggtcaacctc
    84001 atcagttggg cggtcttgcc cagaagatga gggaagttgg gaatctatgg cgctcatcag
    84061 tgtttctcct ttcgcccgcc agagtaaaaa gcaaccgttt acgttaatga aactgtctaa
    84121 acacatgctc gtggagtgcc agtttttgcg aggaagggga gttagagaca ggaattgcgg
    84181 aggcaatcac ataatcgtgc ttaaactacg aaaatgacaa gagaacctga caatggaatt
    84241 atctgtaatt ttctcatatt gatatttcat taatcattct ggcatgtcgc actctcgcat
    84301 ttaatcgttt ttatctggat agcgctattt ggggcggcaa tgttgaaata atgaaagcgg
    84361 catagattga ggggcaataa tttgaactca gtcgcaaaat tataattaaa acggtcatgc
    84421 tacctaatat atgcccgttt attgcatcgc gaaatttgag aagacataac tcattaaatg
    84481 aaagggatgc aaatgaataa gtttatcgct gctgaagccg cggaatgtat aggctgtcat
    84541 gcttgtgaaa ttgcctgtgc ggtggcacac aatcaagaaa actggccgct gagtcacagt
    84601 gactttcgac cacgtatcca cgttgtaggg aaaggcctgg ctgcgaatcc ggtggcctgc
    84661 catcactgca acaatgcccc ttgcgttacg gcttgtccgg ttaatgctct gactttccag
    84721 tccgatagcg tacaactgga cgagcaaaaa tgtattggtt gtaaaagatg cgcaatcgct
    84781 tgcccctttg gcgtcgttga gatggtcgat acgattgcgc agaaatgcga cctttgtaac
    84841 cagcgcagtt ccggcacgca agcctgtatt gaagtttgcc caacacaggc gttacgactg
    84901 atggacgata aagggttaca gcagataaag gtggcccgcc agcgcaaaac ggcagcagga
    84961 aaagcgtcat cagacgctca gccatctcgc agtgcagcgt tgctccccgt taactcgcgt
    85021 aaaggcgcag ataaaatttc agcaagtgaa cggaaaaacc actttggcga aatctattgc
    85081 gggctggatc cacaacaagc gacttatgag agtgaccgct gtgtttattg tgccgaaaaa
    85141 gctaactgca actggcattg cccgctgcat aacgctattc cggattacat ccgtctggta
    85201 caggagggaa agattattga agcggcagaa ctttgccacc agaccagttc cttaccagaa
    85261 atctgcggca gggtatgtcc acaggaccgt ctttgtgaag gcgcatgtac tttgaaagat
    85321 cactctggcg cagtctctat cggtaatctg gaacgctaca tcaccgatac cgcgctggcg
    85381 atgggctggc gtcccgatgt cagcaaagtt gttccccgta tcgagaaagt ggcggtgatt
    85441 ggcgctgggc ctgcagggtt agggtgtgct gatattctgg cgcgcgcagg agttcaggtc
    85501 gatgtctttg atcgccatcc agaaattggc ggtatgctga cttttggcat tcctcctttc
    85561 aaactcgata aaacggtatt aagccagcgg cgagagatat tcaccgcaat gggaatcgac
    85621 tttcatctta actgtgaaat tggccgcgat attaccttta gcgatttaac ttctgaatat
    85681 gatgcagttt tcatcggcgt ggggacttac gggatgatgc gagcagatct gccgcatgaa
    85741 gatgcgcccg gtgtcattca ggctctaccg ttcctgactg cccatacccg ccagctcatg
    85801 ggattgccgg agtctgaaga gtatccgctg acggacgtgg aaggtaagcg agtcgtggta
    85861 ttgggcggtg gcgatacgac aatggattgt ttgcggactt ccattcgcct caatgctgcc
    85921 agcgtgacct gcgcgtatcg tcgtgatgaa gtcagtatgc cgggctcgcg caaagaggtg
    85981 gtcaatgcgc gcgaggaagg tgtcgagttt caattcaatg ttcagccgca atatatcgct
    86041 tgtgacgaag atgggcgttt aactgcggtg ggcctgattc gtaccgccat gggtgagccg
    86101 gggccggatg gtcgccgtcg tcctcgtccg gttgcgggtt cagagtttga attgcccgcc
    86161 gatgttctca ttatggcctt tggtttccag gcgcatgcca tgccgtggtt gcagggcagc
    86221 ggaattaaac tcgataaatg gggcctgatt caaaccggtg acgtcgggta tttacctacc
    86281 cagacgcatc tgaaaaaagt ctttgctggt ggtgatgcag ttcatggcgc ggatctggtt
    86341 gtcactgcaa tggccgcagg aaggcaggcg gcgcgcgata tgttaactct gtttgatacg
    86401 aaggcatcgt gatgaaatcg ttaattatcg ttaatccggc tgactgtatt ggctgccgta
    86461 cttgtgaagt ggcctgtgtg gtcgctcacc cttcataaca ggagttgaat gccgatatct
    86521 ttttaccccg gctgaaggta cagcgactgg actgcatcag tgcgccggtg atgtgccatc
    86581 agtgtgaaaa cgccccttgt gttggcgctt gccccgtggg ggcgctgacg atgggcgagc
    86641 aggtggtgca gaccaattct gcccgttgta ttggctgtca gagttgcgtc agtgcgtgcc
    86701 cgtttggaat gatcaccatt cagtcgttgc cgggggatac ccggcaacaa atcgtgaaat
    86761 gcgatctctg tgaacagcgg gaagaggggc ctgcctgcgt tgaatcttgc ccaacacagg
    86821 cgctgcagtt gcttactgaa agagaactca ggcgagtccg ccagcagcgt attgttgcca
    86881 gcggtgagaa tcctctgtga aagaaatctc cgcgctgaaa ggcgcggaga atctattaga
    86941 ttgcccaacc acccgcgtaa aatgcgacca gtgcggcagt aatgatcacc gtaccaatat
    87001 tcagcttttg ccattcgcgt gcaaagacgc ggcctacgac cagtgtcaca aagcccagca
    87061 taataccggt aacgatatta caagtcagaa cgatgaacac ggcacacacc aggccagcca
    87121 tagcgtcaat aaaatcattg aaatccagct tcgagacgtt acttaacatc agcaaaccta
    87181 cgtacatcag agcgggtgca gtggcgtaac cagggatcaa aaatgacagc ggtgataaga
    87241 acagaatcag caggaataac gcccccacta cggttgcggt taaccctgtt ttacccccgg
    87301 cggcggttcc tgccgctgat tcgatataaa ccgctgcggg cgctgcgccc accaggccgg
    87361 agaatattga acttactgag tcactggtca gggctttgcc gccgttgatg atctggttgt
    87421 ctttatccaa caaattcgcc tgaccggcga cggcacggat ggtgccagta gcgtcgaaca
    87481 ctgcggtcat caccaatgcc agtacactcg gaagtacagt tggctggagt gcgcccataa
    87541 tatcgaggct gaaaatcaga gacttaccat cttcgccagt caggcttggc atcgccacca
    87601 gaccgtggta tttcaccgct gggtcaaaga ttaagccgat gatcgaaatt gcaataatca
    87661 ccaacaagat accgccgggt acgcgacact tctccagacc gaagatgacc gccagcccca
    87721 gcaagctcat catcaccggg aaggaggtaa acgcaccgag cgccactggc aagccttcaa
    87781 tcgggttttt gataaccata cccacaccgt tagcagcaat cagcagcaga aacagcccga
    87841 taccgatacc tgtaccgtga gcgataccca tcggcaaatt acgtaagatc caggtacgca
    87901 caccggttac ggaaatggcg gtgaagatga cgcccatcag aaataccgcg cccagtgcga
    87961 caggaacgct aatttgttgc ccgagtacca gactgaatgc ggtaaacgcc gtcaaggaaa
    88021 tcgcgcaacc aatcgccatt ggcaaattag cccataaccc catcagcaac gagccgaagc
    88081 ccgcgaccag acaggtggca acaaacacag ctgcgggagg aaaacctgct ttgcccagca
    88141 ttcccggaac gacgataacg gaataaacca tggccagaaa ggtcgttaag ccagccagta
    88201 cttcctgacg aacggtactg ccacgagcgg taattttaaa ataattatcc agcgcgccct
    88261 gtggctttgg tgcgtccggt gtttgtagga tgtctccaga cataatgatg tcctcacgag
    88321 gatgtaaaag ttttattatt agttgcgttc gtacaccaga cgaccatcaa cgtaggtgcg
    88381 gtagatcgaa cggtcatcgc ccaacgtcat catcacgaac aatttgtcga ctaaagaaac
    88441 agagttgtca tagcgcagct gctgtagcgg agtggcggtg ggttccatca ccacgaaatc
    88501 agcctctttg ccaggtaaaa agttgccaat caaatcgtca aggcccagag atttcgctcc
    88561 gccgagcgtg gccaggtaaa acgcttcata tgccgagagg cgatagcctt gtaattgcaa
    88621 cactttgtag gcttcgttca gcgtttgcag catgttgaaa gtggttccgg caccgatatc
    88681 cgttcccatg cccactttaa ctttcttctg ccatgctttt ttcaagttga ataacccgct
    88741 gccgaggtaa aggttggagg tcggacagaa agcaatgctg gatttggttt cgctgagacg
    88801 atcccactct ttttctttga gatggacgca gtgagcaaag acacagtttt taccggtcag
    88861 gccgtactga tggtaaacat ccagataacc atcatggtca ggataaagcg atttcaccca
    88921 ggcaatttca tctttgtttt cacagagatg ggtatgtacc cacgtatccg gatattcctc
    88981 tttcaggcgt tgcgccatcg ccatctgttc aggagaagaa gtaggggcga agcgtggcgt
    89041 aatcgcatat agcagacgac catttttgtg ccagcgttca atcagttctt tgctttggtg
    89101 atagctgctt tcggcagtgt ctagcagata atccggtgcg ttgcggtcca tcatcacctt
    89161 accggcaatc atacgcatat tgatatgact ggcggcttca aacagcgcat caacggattg
    89221 cggatgaaca gtgccaaaca ccagcgccgt ggtggttccg ttacgtaaaa gctgcttgat
    89281 gaagaacgcc gacatttcgc gggcgtactc taaatcctca taacgacgtt cagtagggaa
    89341 ggtgtgttta ttcaaccact ccagcaattg ttccccatag gcccccacca tttcactttg
    89401 cggataatgg atatgtgtat cgacaaagcc cggtactatc agtttgccgc gatagtcgcg
    89461 cacgcgaata gtgtcaggaa tttgatgctt tccgtcttcc cattcgccaa accattccac
    89521 ttttccctgt ttaatgagta ataaaccatc ctcaataaac cgcagcgcag aggcaatctc
    89581 ttccgggtta tcgaccgtac gggtgacatc aataaaactg cctcgtaccg cttttaacgt
    89641 gtgttctcct gacatcattg actcctttac tgttaatcca tctcttccgc tgaggtaata
    89701 ccaggcagaa cgttttcctg tcggtagcca ccagggagga taatatttaa taaaatcgca
    89761 gttaacccgc cagcacaaat agggttttca actaatacat aaatagaggc tggcaatatt
    89821 ttaaaaattt cgggatcgta ggagacgcca agtcctaaac ctaaagaagt ggcgacaata
    89881 agtgtttctc ggcgctttaa accgttggtg atgatgatgc gaatcccagc gatggcaatc
    89941 atggaaaaca tcaacgtcat tgcgcctccc agaactgccg agggaatggt cgtgaagaag
    90001 ccgccaatca tcggaaacaa accgaggata accagcatta ccgcgatggt tcgcccgaca
    90061 taacgtgaag cgacgccagt catctgaata accccattat tttgcgcaaa cgtggttaag
    90121 ggtaatgacc cgacagcgga ggcgataaca gaaaccagac cgtctgccag cacgccgcct
    90181 ttcagtcggg actgatactc ttctccctga atgggtcggc gggaaaccat tgccgtggcg
    90241 gtgatatcgc cgacagcttc cagcacgcta agcagataaa tcgtgccaac caccaggaac
    90301 tgatggaagc taaaactaaa gccgtatttg aacggatgtg ggatggtgat taacggcaaa
    90361 ttgcgcatac tgctgaaatc caccatgccc aggcataacg acgcgatata accgacgcat
    90421 agcccaatgg cgatccctcc catgcgtagc aacggactgc gacagcagtt aaagccgatc
    90481 accacaatca aaaccaataa accaacgccg agatgttcgt aattgccgaa ggtaccgctg
    90541 cttttggcag caaatcctcc accaaaatcg ataatgccga ctttaatcag gcttaagccg
    90601 atcatcagta ccacaatacc gctgacagta ggcgtaataa cccgacgtaa atagggcaag
    90661 ataaatgaag atccgacaac cagaaatgcg ccaacgaagg agacgccgag gagcgacgac
    90721 atgattaact cttcgtgaaa accgtcgctt ttcatgctgc tgcccagcgc aatcatgacc
    90781 gtaacaaatg aaaaattgac tgactgaatt gagagtaggc cagaaccgac gatgccatag
    90841 cggtttactt gtaaccaggt accaatacca gaggcgatca tcgccattga aacaagatag
    90901 gcagttgttt cagcggaaag ctgtaaggcc gcccccacga ttaacgcggg ggttaccatc
    90961 ggaacgaaaa ttgccaacag atgggtaatg gcaccaacga gagcctgatg aaagggaggg
    91021 cgatcctcca gttcaaatat aaggtcagaa cctgcatggt ttatatcaga catcccttca
    91081 ctccttttaa agtaaatttg atcatctgaa ttagtaaaat aaattcccac gaatttgggg
    91141 cgcaggacgc cttgagaacg gcttatctca ttttcgcagt cactatttag tcatcgtcca
    91201 aacagagaaa caggccgccg aagcggccat ttattattta tatcttttcc agcgcggtga
    91261 gtattttctc cggtgtgaaa tgccattcgc gtaaccagat gccgcatgca tcgtgaattg
    91321 cggtagcaat cgccggagct gcgccattta caccgatttc cgagatcgat ttcgccccga
    91381 acgggccgac tttatcgtcg ctcggcacca gcactgcgcg gaaatcacgc ggaatgtcac
    91441 caattttcgg tgcgccgtag ctgcgtaaat cacgcgttaa cgggtgacct tcggcgtcat
    91501 aaatgatctc ttcgctcata ctgtggccga tagctcgcag ggtagcaccg tagatttgtc
    91561 ccaacgctaa ctctggattg accggtgtac cgcagtccag caaggcgtag aatttatcca
    91621 ggcggatttc acccgtacgc gtgttgacgg caacttcagc gaagtttgcg ccatacggga
    91681 aggcgaaatc aggcgtgata taactgccag ttcccaccag tgaaccaaag ccggtgccgg
    91741 tttcgccttt atgggcaata tccccgaaac taacttcgcc tttcttgccg cgcacgacgc
    91801 ccggcgttgc tagttgaaca tctgccactg gctcacccaa catttgcgcg ccgtggaaca
    91861 gaattttctc ccgcagattt tccgctgcca aacgcgccgc gttacccgag aagcaagtac
    91921 cggacgaggc atatgcgcct ttatcaaaca acgcatgatc ggtatcaccg gagataacat
    91981 gcacgtcctg cggtgggcag tgcagcactt ctgctgccag tttcgtcacc acggtatcca
    92041 gaccagtacc aatatccgca ccgccagaat gaacgataaa ggtaccgtct gattccagtt
    92101 tgatcatgca gttagcctga tcgatatccg ggatccctga tttctgcatg ataatggcga
    92161 caccacgacc gatgtgccag tcaccatttt gtggttttgg tgaagaccat tggatcatct
    92221 cgcgcccctg acgcaggatc tcttccagtg cgcagctggc ggcggaagga acggaggtcg
    92281 gcgctttacc ttcaccgatt gcaccgagaa ttttcagctc ttgcccttcg tgtacccggt
    92341 tacgttcgat aatttccagt tggtcgatct gtaactgttc agccagttcc gctaatgcca
    92401 tggtgatagc gaagttacct ttcggtgcgc cataaccctg ataagcacca tttgggcaaa
    92461 tgttgctgta gtaggtggtg acctggaaat cgacgttatc gcacggatat aacggcagcg
    92521 acagcgccgg tccgttacac ggtacggtga gtgagtggtt gccgtaaggg ccagtgttgg
    92581 cgcggaaatc catcttcact gccgtcaggc gaccatcttt tttcgctccc agtttgacgg
    92641 tgactttcgc gacgtgacga gaggtgttag caataaactc ttcttcacgg gtgtagcgga
    92701 acagtaccgg acgcccggtc acgcaggttg cccaggcgca cacttcttcc agcaggatgt
    92761 cctgtttgga accaaaaccg ccgccaactc gctctttaat gacatgaact ttatgctgtt
    92821 tcatgtccac gaggcgcgcg acctggcggc gtaagtgcca tggtacctgg gtggaggcgt
    92881 ggataaccag acgatcgccg tccatccggg taaagcagat atgtgtttca gtcgggcact
    92941 gctgcgcctg cgttgagtta taggttcgct caatgatcac atcggcatcg gcaaagcctt
    93001 tgtccatatc gccaatatga ccatgaatac tagcggcgat atttttgcga gggcgagaac
    93061 cgatcgggaa gttgatgatc atatgctcgc cgcgctgggc tgcatggctg ttatcgtctt
    93121 ccagagtatc tggcgcacca gcaacataca ccaccggttc atcgtgcacg acaggcgcat
    93181 cttccgccat tgcttcgtcg atcgacatta ccggcttaag cacttcatat tcaacctcga
    93241 tgagtttcaa tgcttcgagc gcaatttctt cactttcagc gaccaccgca gcaacgcgat
    93301 cgccgacgtg acgcattttt ttgccgaaca tacggcggtc aagcggtgac ggttccggtg
    93361 cgctctgacc ccccggggta tagtagatat cagggcaatt caggtgagta ataacgtgaa
    93421 cgacgcccgg taaggcttca gctttgctga catccagatg agtaatcagt gcgtgagcgt
    93481 gtgggctacg taacatttta atgacgcagg cgtcagccgt tacgcggtct tcaacatagc
    93541 agggtttcgc ctgcaccatt ttcgcggcat cagttttagg ataatgcttg ccaatgactt
    93601 caaggtcgtc acggaaagtc ggagcgatat caatggtggc ctgcggatta tttttacgtg
    93661 caaccgccag ttcaatgacc tgatagtatt gctgccagcc agcatcgcgg ctgaacaaac
    93721 cagaaagcgc gtcgtcgatc tcttcgcggg taggtgcggc gatgcgatcg agaagatcgg
    93781 tgataatcag agctgcagct ggatcgttat aaccagactg caccacgcca acatcaacca
    93841 ttgcctgttg aaccagactt aactcgttcc atttgcccag agattctgcg gtacgaatat
    93901 ctgccttctc taactgtgcg gcaataagca aggacgcgtt aacgatatta ccgttaaaga
    93961 tgattgcgtc agaaccggca aacccgaaac catcatcact gttgcgtaca gagtgcattc
    94021 ccatgttaaa caacagcttt tgcacgtttt cgcctggatt aacggttagc tcctgaggcg
    94081 cgccatttaa agtaaagtgg atgatcatac ggcttcctcc ccagcctgtt ggcagtcggc
    94141 atacaggtcg gctaccagaa ctcccgtgat atagcgttta taggccacgc tgccccgcaa
    94201 atcttcctgc gggaaaatgg cgttggcgac agcctgttca agtgcattgc cttccagatt
    94261 ttgtttttcg acatcatgca gacgcagtgc tttactggcg acgccatcca gcgcaattcg
    94321 cataccgtcg tggtctgtca ttgcaacggc tgccgtcacg acggttaaac ctgcctgaga
    94381 acggctaatt ttgcgggtcg cacaggtgcg atacggatct ttaatgataa tttcggttag
    94441 caggcgatcg catgggcagg ccaggtagtc ctcgattgac agcgtttcgc cgttgccaaa
    94501 aaccagttca gcatccagtg ccagcaggac gggaagcagc actgactctt cctgccgggc
    94561 ggcgatttca ccaccaatgg tcgactgatt acgaacatgg cgtgagtaaa caaaaccgag
    94621 ggcttcacgc agtgctgcag gaataaatcg cgcatcacgc agtggctgca agcgagacat
    94681 tgcgccaatc cgcagtgcac cgttatccca gtcaacccag tccagttcca gatcctgtaa
    94741 ggaaatggca atctttttat cggtacgggt tggtgtagca ttgagtttgc tgcccccggc
    94801 gaaccagacg gcttcatcct ggtagcggcg cttcagttcc agcgcctgtt cgacagagtc
    94861 gggcctgaaa aattgttcaa tcatcttctt tccttattta accctcttcc tgcgggaaga
    94921 gggcatctgt catttatgcc agcgcatcca tccgacgcca catactggca gcggcttttc
    94981 tggcttgtgc ataaatggaa tcgcaatcga agttaaactg acggtcttca tagaccatca
    95041 caccattgac catcacgctg tgaacgctgc ctgagcccat accgaaagcg atatgcccgg
    95101 cgatattgtc tgccagcagc ggcgtcggcg agttgtaatc gcaaatggtt aaatcagctt
    95161 tgtaaccggc ttccagaagc ccaaatttcg cgccaaagtt gcggctcatc agttcgttac
    95221 cgttagtcag ggctttggca aaactgtcag gccacagcgg accacccgca tcgcgatgtt
    95281 taaagaaggc aaatttcatc tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg
    95341 ccagattgcg gatgtcgcta aggtgatggt tgtagccgac atggttgttc atgtttgaac
    95401 gggcgttatg caccaggaac gcatcgcgct gattgagtag ggcgatgtca tctttcgaca
    95461 agtacagccc atgagcgacc agcgttttgc tatcgatgag atcgaattgc gccagtcgtg
    95521 ccagcaggtc tttgccgtac cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag
    95581 cgtgaatatg caaaccacgg cctgtggcgt tcacggcttc acgtagcatc tccagaccgg
    95641 catctggcac ggtaaacggc gcgtgagcac cgatatgtgc ttccaccaga tacggctcgg
    95701 ttgctgcttt cttcgcctca tcaatctgac gggcgaagcg gatgttttct tctacacctt
    95761 cctgcaactc tttgatgccg ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca
    95821 ggccaacttt taaaaatgcg tcgcgcaatg tggagagcga cccgccgata tacgccggag
    95881 aggcatggtg atcgataacc gatgtacatc cgctcttaat cgcttccagg gaacaaatca
    95941 gcccgctgta atagagcgac tcttcatcaa gggcgcgatc gagccgccac cagagatttt
    96001 tcagcgttga gatgaaatct gggctggggg cgatgtttgc cataattccg cgggaaagcc
    96061 ccgagtaaaa atggttgtgc gagcagacaa ttcccggcat cacaatccga ccatgcatct
    96121 ctttgtagct ggcatcgggg tagcgttgcg tcagggcatc gccgatagcg acaatcacat
    96181 cattttcgat agcgatatca acgccttcct gcactttcgc cgggtgtaac tgcactgcgg
    96241 tgacattctt cagaatcaac atgattacac ctccacgcgg cccagcagat aatgatgatg
    96301 ctggtggaca tggctgatga tgcggcacat atcgttcagc tccggtggta cgttgttgaa
    96361 ctgaccttcg ctgtcgatgt ttaacaccca gctttggtta ttcagtcgta ctcgtacccg
    96421 gcaatcttcc acaaggaagc ctgggttgct gctgttatca aagtcttgcg ccaggctgaa
    96481 aacggtgatt ttgtctttgt atggtttacc gttccacgga cagaactgag cgcagttgcc
    96541 gcattcgtta cagtaagcgt cgaggtgcag cgtctggaat ctattctgga agcctgggac
    96601 cgcaatggag acgttggcgc ggttcgggca gacatccaca cacttgctgc aaacgtagtt
    96661 acattcaagg cagcgagcag cttcctgcgc gacaaacgca tcacgatcgt cactgttcac
    96721 cagcgtgata gagatatcgc ctttacgttg atagatttcc gccggattga cgttgttcca
    96781 gtatttatcg ttctggtggg aacggatatt ttcccgacta aggatggcat cggtcgcccg
    96841 acgcgcggtt ccgacagcag cgacaatgga ggatggtccg cgctgtacgt cgccgatcat
    96901 aaagacgtca gtcagacgag tttcgccatt atggtcgacg tctggccagc cgtttttgtc
    96961 cagcggcacg cccatcgcat tcagggcttc agtatcctgc tgttcaccaa tggcggtgat
    97021 caggctgtct acatgcagtg ttactgtttc gttggtttca accggacgac gacgaccttt
    97081 ctcatcgggt tcgccaagcg acataacgcg caaggttaag gtgccatcag catcgaaacg
    97141 ttccggatta ttcaggaaac ggaactccac gccgtcgtgc aacgcttctt catactcttc
    97201 gcgccatgcg ggcatctctt gcagtgaacg acggtaaacg accgttgctt tttctacgcc
    97261 tggaacgcgt aacgccgcac gagcgcagtc cattgcggtg ttacccgccc cgacaacgac
    97321 cacatgtttg cccagcttga gcgctgtacc cttgttgtat tcacgcagga aggggagtga
    97381 tttcaagaca ttttggttgt cgcccgccag tttcacaccg ctatttttat ctgtgccggt
    97441 ggcaatcaga acatagtgga agtcctgatt ttttaactgc tcaacggtta aatcgggtga
    97501 gcagccatac tcaaatttca cgccgtgagc ggcaacaaaa tcgatatcgt gctgaattaa
    97561 ctcagccgga attcggaact gaggaatgat atttttcacc acgccgcccg cattggcttc
    97621 gcgttcaaac agcgtaaccg gatggcccgc tctggcaagg aagtaacctg ctgccagacc
    97681 cgccggacct gcaccaatca cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg
    97741 ccagcgttgc ttatattcat cccaaccttt ttccagcgcg acttttttca gttcgcggat
    97801 attcagcgca ctgtcgtaat ccaggcgggt acagttgtat tggcactggt gatcgcaaat
    97861 atgaccggta atggcgggca gagcgttgcg ttggtagatg agttccagcg cgtcggcata
    97921 gcggtgttcg ccaagcagac ggatgtattc cggaatatct tgcttgatag cgcaggcagt
    97981 aacacagggg gcaacgtagc agtcggtcag cggcaggtct tctgccactt caatacgctc
    98041 ttctggcttc cagtgtttct gggtgtattc catggttaac gcatctgctg ccagtctgtt
    98101 cagtcgttcg acgtcaacat ggtcaagtcc ccaggcgtcg gagccttcca gctcgcgcat
    98161 gcaggcactt aagcgcaaat agccgccagg tttcagcagg tcggttgcca tagtaatagg
    98221 gcgaatacct gtatcaaaaa tatcgcggat agtcagctga ctggcaccac cggaataaga
    98281 aatgggcagt ttgccgtcaa aggcgcgaga gagaactgct gcaacattga tggagagcgg
    98341 gaacagcgca cggcctgaca tatacatctc ttcaccaggc agtgcgcctt tattgttgat
    98401 ggtgccgaga gtgttagtca gttttacgcc aaagccgagt gatttttctt ttgccagtgc
    98461 catcaggcgt tccagcattt ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc
    98521 ttcttttaag cctatgtaac cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta
    98581 ccccagtaag gtcgggttaa gtttcacaaa ggtgttgagc cctttttctt ccagcatgta
    98641 gcggcaaatg gcttcgattt catgcggagg acagccgtgc atggtggaga gggtgacgcc
    98701 atgcaccata ctggtgggga tgcgagcggg taaggcttgc aagctttcgc gtttttcctg
    98761 caatccgtgg cgggctaaaa atgcgtcatc ctggagtaat ttattcagcg tatcgcgata
    98821 ttgcgcgaat ttcggatggt cagatgcgtc catcatattg tcgatgaact gttgcattgg
    98881 cggttgctta ataccttcga ggttgtaacc gacgctcata ttaaagatga acgatttacc
    98941 ggaatcagaa ggttggaaca tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta
    99001 ttcatcccag gctttaagca gggtaaattc ggtagaccat tcggtgttaa agcactcgtc
    99061 ttcggcatca atacagggct tttccagctc caggcggtca agaatttgga cggtttttag
    99121 ttcgatgaaa cgtccgccag tcagccagga agtgacaata ttttgcgcga gctgcgtgtg
    99181 cgggccagcg gcagggccga cgggagtggc acaggtttca ccgaagacgc taacagtttt
    99241 accttttaca ggtgagtaaa actgttgctc gggaatacca aagattgagc gttgttgttg
    99301 gtattcatca aatatgcgcg tcaaaagttc ctcaaacgga atgggacgca taatatcccc
    99361 cataaccctc tccttagctt atacagatat cattgagtga ccggtgttga gaaatccggt
    99421 ggtttagggg gatagagcaa caatcgcacc actcttgcca ggtttggcgg gaaatataaa
    99481 aaaatgtgag gcggctcgaa acttaagggc tttttgtgaa tgaactcaca ggtgctaaat
    99541 gagaattaaa atcactcctg aaaattatta ttgccatccc atatatcact ttttctcttt
    99601 tctcaaaatc agtga
//
LOCUS       DAZFTN010000005        97580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFTN010000005 DAZFTN010000000
VERSION     DAZFTN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..697)
                     /gene="emrY"
                     /locus_tag="ACHKV2_000415"
     CDS             complement(<1..697)
                     /gene="emrY"
                     /locus_tag="ACHKV2_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6938373.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDW"
     gene            complement(697..1860)
                     /gene="emrK"
                     /locus_tag="ACHKV2_000416"
     CDS             complement(697..1860)
                     /gene="emrK"
                     /locus_tag="ACHKV2_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO6938374.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            2276..2890
                     /gene="evgA"
                     /locus_tag="ACHKV2_000417"
     CDS             2276..2890
                     /gene="evgA"
                     /locus_tag="ACHKV2_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO6938375.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            2895..6488
                     /gene="evgS"
                     /locus_tag="ACHKV2_000418"
     CDS             2895..6488
                     /gene="evgS"
                     /locus_tag="ACHKV2_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO6938376.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(6544..7685)
                     /gene="yfdE"
                     /locus_tag="ACHKV2_000419"
                     /pseudo
     CDS             complement(6544..7685)
                     /gene="yfdE"
                     /locus_tag="ACHKV2_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(7759..8703)
                     /gene="yfdV"
                     /locus_tag="ACHKV2_000420"
     CDS             complement(7759..8703)
                     /gene="yfdV"
                     /locus_tag="ACHKV2_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO6938377.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(8773..10467)
                     /gene="oxc"
                     /locus_tag="ACHKV2_000421"
     CDS             complement(8773..10467)
                     /gene="oxc"
                     /locus_tag="ACHKV2_000421"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO6938378.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(10521..11771)
                     /gene="frc"
                     /locus_tag="ACHKV2_000422"
     CDS             complement(10521..11771)
                     /gene="frc"
                     /locus_tag="ACHKV2_000422"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO6938379.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(12284..12919)
                     /gene="yfdX"
                     /locus_tag="ACHKV2_000423"
     CDS             complement(12284..12919)
                     /gene="yfdX"
                     /locus_tag="ACHKV2_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO6938380.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            13215..13490
                     /gene="ypdI"
                     /locus_tag="ACHKV2_000424"
     CDS             13215..13490
                     /gene="ypdI"
                     /locus_tag="ACHKV2_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO6938381.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(13567..13809)
                     /gene="yfdY"
                     /locus_tag="ACHKV2_000425"
     CDS             complement(13567..13809)
                     /gene="yfdY"
                     /locus_tag="ACHKV2_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO6938382.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            14162..15082
                     /gene="lpxP"
                     /locus_tag="ACHKV2_000426"
     CDS             14162..15082
                     /gene="lpxP"
                     /locus_tag="ACHKV2_000426"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO6938383.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            15438..15509
                     /gene="ypdK"
                     /locus_tag="ACHKV2_000427"
     CDS             15438..15509
                     /gene="ypdK"
                     /locus_tag="ACHKV2_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO6938384.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(15574..16812)
                     /gene="alaC"
                     /locus_tag="ACHKV2_000428"
     CDS             complement(15574..16812)
                     /gene="alaC"
                     /locus_tag="ACHKV2_000428"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO6938385.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            17189..18886
                     /gene="ypdA"
                     /locus_tag="ACHKV2_000429"
     CDS             17189..18886
                     /gene="ypdA"
                     /locus_tag="ACHKV2_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO6938386.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            18901..19635
                     /gene="ypdB"
                     /locus_tag="ACHKV2_000430"
     CDS             18901..19635
                     /gene="ypdB"
                     /locus_tag="ACHKV2_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO6938387.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            19648..20505
                     /gene="ypdC"
                     /locus_tag="ACHKV2_000431"
     CDS             19648..20505
                     /gene="ypdC"
                     /locus_tag="ACHKV2_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6938388.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(20508..23003)
                     /gene="ptsP"
                     /locus_tag="ACHKV2_000432"
     CDS             complement(20508..23003)
                     /gene="ptsP"
                     /locus_tag="ACHKV2_000432"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6938389.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(23028..24065)
                     /gene="ypdE"
                     /locus_tag="ACHKV2_000433"
     CDS             complement(23028..24065)
                     /gene="ypdE"
                     /locus_tag="ACHKV2_000433"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6938390.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(24065..25150)
                     /gene="ypdF"
                     /locus_tag="ACHKV2_000434"
     CDS             complement(24065..25150)
                     /gene="ypdF"
                     /locus_tag="ACHKV2_000434"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6938391.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(25165..26412)
                     /gene="fryC"
                     /locus_tag="ACHKV2_000435"
     CDS             complement(25165..26412)
                     /gene="fryC"
                     /locus_tag="ACHKV2_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6938392.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(26434..26760)
                     /gene="fryB"
                     /locus_tag="ACHKV2_000436"
     CDS             complement(26434..26760)
                     /gene="fryB"
                     /locus_tag="ACHKV2_000436"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6938393.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(26979..27944)
                     /gene="glk"
                     /locus_tag="ACHKV2_000437"
     CDS             complement(26979..27944)
                     /gene="glk"
                     /locus_tag="ACHKV2_000437"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO6938394.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            28148..29404
                     /gene="yfeO"
                     /locus_tag="ACHKV2_000438"
     CDS             28148..29404
                     /gene="yfeO"
                     /locus_tag="ACHKV2_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO6938395.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            29519..29845
                     /gene="ypeC"
                     /locus_tag="ACHKV2_000439"
     CDS             29519..29845
                     /gene="ypeC"
                     /locus_tag="ACHKV2_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO6938396.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(29985..31223)
                     /gene="mntH"
                     /locus_tag="ACHKV2_000440"
     CDS             complement(29985..31223)
                     /gene="mntH"
                     /locus_tag="ACHKV2_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6938397.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            31559..32761
                     /gene="nupC"
                     /locus_tag="ACHKV2_000441"
     CDS             31559..32761
                     /gene="nupC"
                     /locus_tag="ACHKV2_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO6938398.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(32811..35000)
                     /gene="pdeA"
                     /locus_tag="ACHKV2_000442"
     CDS             complement(32811..35000)
                     /gene="pdeA"
                     /locus_tag="ACHKV2_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6938399.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(35208..35283)
                     /locus_tag="ACHKV2_000443"
     tRNA            complement(35208..35283)
                     /locus_tag="ACHKV2_000443"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35248..35250),aa:Ala,seq:ggc)
     gene            complement(35323..35398)
                     /locus_tag="ACHKV2_000444"
     tRNA            complement(35323..35398)
                     /locus_tag="ACHKV2_000444"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35363..35365),aa:Ala,seq:ggc)
     gene            35619..35978
                     /gene="yfeC"
                     /locus_tag="ACHKV2_000445"
     CDS             35619..35978
                     /gene="yfeC"
                     /locus_tag="ACHKV2_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6938400.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            35980..36372
                     /gene="yfeD"
                     /locus_tag="ACHKV2_000446"
     CDS             35980..36372
                     /gene="yfeD"
                     /locus_tag="ACHKV2_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6938401.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(36412..37620)
                     /locus_tag="ACHKV2_000447"
     CDS             complement(36412..37620)
                     /locus_tag="ACHKV2_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6938402.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            37628..38053
                     /gene="tnpA"
                     /locus_tag="ACHKV2_000448"
                     /pseudo
     CDS             37628..38053
                     /gene="tnpA"
                     /locus_tag="ACHKV2_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(38171..39586)
                     /gene="gltX"
                     /locus_tag="ACHKV2_000449"
     CDS             complement(38171..39586)
                     /gene="gltX"
                     /locus_tag="ACHKV2_000449"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO6938403.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            39845..39920
                     /locus_tag="ACHKV2_000450"
     tRNA            39845..39920
                     /locus_tag="ACHKV2_000450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39878..39880,aa:Val,seq:tac)
     gene            39965..40040
                     /locus_tag="ACHKV2_000451"
     tRNA            39965..40040
                     /locus_tag="ACHKV2_000451"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39998..40000,aa:Val,seq:tac)
     gene            40087..40162
                     /locus_tag="ACHKV2_000452"
     tRNA            40087..40162
                     /locus_tag="ACHKV2_000452"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40120..40122,aa:Val,seq:tac)
     gene            40167..40242
                     /locus_tag="ACHKV2_000453"
     tRNA            40167..40242
                     /locus_tag="ACHKV2_000453"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40200..40202,aa:Lys,seq:ttt)
     gene            40362..40703
                     /locus_tag="ACHKV2_000454"
     CDS             40362..40703
                     /locus_tag="ACHKV2_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO6938404.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(40694..41620)
                     /gene="yfeR"
                     /locus_tag="ACHKV2_000455"
     CDS             complement(40694..41620)
                     /gene="yfeR"
                     /locus_tag="ACHKV2_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6938405.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            41710..42708
                     /gene="yfeH"
                     /locus_tag="ACHKV2_000456"
     CDS             41710..42708
                     /gene="yfeH"
                     /locus_tag="ACHKV2_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO6938406.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(42705..42923)
                     /gene="ypeB"
                     /locus_tag="ACHKV2_000457"
     CDS             complement(42705..42923)
                     /gene="ypeB"
                     /locus_tag="ACHKV2_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO6938407.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(42925..44940)
                     /gene="ligA"
                     /locus_tag="ACHKV2_000458"
     CDS             complement(42925..44940)
                     /gene="ligA"
                     /locus_tag="ACHKV2_000458"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO6938408.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(45011..46009)
                     /gene="zipA"
                     /locus_tag="ACHKV2_000459"
     CDS             complement(45011..46009)
                     /gene="zipA"
                     /locus_tag="ACHKV2_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO6938409.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            46239..47000
                     /gene="cysZ"
                     /locus_tag="ACHKV2_000460"
     CDS             46239..47000
                     /gene="cysZ"
                     /locus_tag="ACHKV2_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO6938410.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            47185..48156
                     /gene="cysK"
                     /locus_tag="ACHKV2_000461"
     CDS             47185..48156
                     /gene="cysK"
                     /locus_tag="ACHKV2_000461"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO6938411.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            48540..48797
                     /gene="ptsH"
                     /locus_tag="ACHKV2_000462"
     CDS             48540..48797
                     /gene="ptsH"
                     /locus_tag="ACHKV2_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO6938412.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            48842..50569
                     /gene="ptsI"
                     /locus_tag="ACHKV2_000463"
     CDS             48842..50569
                     /gene="ptsI"
                     /locus_tag="ACHKV2_000463"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO6938413.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            50610..51119
                     /gene="crr"
                     /locus_tag="ACHKV2_000464"
     CDS             50610..51119
                     /gene="crr"
                     /locus_tag="ACHKV2_000464"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO6938414.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(51162..52013)
                     /gene="pdxK"
                     /locus_tag="ACHKV2_000465"
                     /gene_synonym="thiJ"
     CDS             complement(51162..52013)
                     /gene="pdxK"
                     /locus_tag="ACHKV2_000465"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO6938415.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            52118..52485
                     /gene="yfeK"
                     /locus_tag="ACHKV2_000466"
                     /pseudo
     CDS             52118..52485
                     /gene="yfeK"
                     /locus_tag="ACHKV2_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(52488..53399)
                     /gene="cysM"
                     /locus_tag="ACHKV2_000467"
     CDS             complement(52488..53399)
                     /gene="cysM"
                     /locus_tag="ACHKV2_000467"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO6938416.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(53533..54630)
                     /gene="cysA"
                     /locus_tag="ACHKV2_000468"
     CDS             complement(53533..54630)
                     /gene="cysA"
                     /locus_tag="ACHKV2_000468"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO6938417.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(54620..55495)
                     /gene="cysW"
                     /locus_tag="ACHKV2_000469"
     CDS             complement(54620..55495)
                     /gene="cysW"
                     /locus_tag="ACHKV2_000469"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO6938418.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(55495..56328)
                     /gene="cysT"
                     /locus_tag="ACHKV2_000470"
     CDS             complement(55495..56328)
                     /gene="cysT"
                     /locus_tag="ACHKV2_000470"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO6938419.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(56328..57344)
                     /gene="cysP"
                     /locus_tag="ACHKV2_000471"
     CDS             complement(56328..57344)
                     /gene="cysP"
                     /locus_tag="ACHKV2_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO6938420.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(57502..58293)
                     /gene="ucpA"
                     /locus_tag="ACHKV2_000472"
     CDS             complement(57502..58293)
                     /gene="ucpA"
                     /locus_tag="ACHKV2_000472"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO6938421.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(58422..59279)
                     /gene="murR"
                     /locus_tag="ACHKV2_000473"
     CDS             complement(58422..59279)
                     /gene="murR"
                     /locus_tag="ACHKV2_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO6938422.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            59443..60339
                     /gene="murQ"
                     /locus_tag="ACHKV2_000474"
     CDS             59443..60339
                     /gene="murQ"
                     /locus_tag="ACHKV2_000474"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO6938423.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            60343..61767
                     /gene="murP"
                     /locus_tag="ACHKV2_000475"
     CDS             60343..61767
                     /gene="murP"
                     /locus_tag="ACHKV2_000475"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO6938424.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            61772..63076
                     /gene="pbp4b"
                     /locus_tag="ACHKV2_000476"
     CDS             61772..63076
                     /gene="pbp4b"
                     /locus_tag="ACHKV2_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO6938425.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(63134..64033)
                     /gene="yfeX"
                     /locus_tag="ACHKV2_000477"
     CDS             complement(63134..64033)
                     /gene="yfeX"
                     /locus_tag="ACHKV2_000477"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO6938426.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(64129..64704)
                     /gene="yfeY"
                     /locus_tag="ACHKV2_000478"
     CDS             complement(64129..64704)
                     /gene="yfeY"
                     /locus_tag="ACHKV2_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO6938427.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(64765..65214)
                     /gene="yfeZ"
                     /locus_tag="ACHKV2_000479"
     CDS             complement(64765..65214)
                     /gene="yfeZ"
                     /locus_tag="ACHKV2_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO6938428.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(65201..65626)
                     /gene="ypeA"
                     /locus_tag="ACHKV2_000480"
     CDS             complement(65201..65626)
                     /gene="ypeA"
                     /locus_tag="ACHKV2_000480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO6938429.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            65840..66709
                     /gene="amiA"
                     /locus_tag="ACHKV2_000481"
     CDS             65840..66709
                     /gene="amiA"
                     /locus_tag="ACHKV2_000481"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO6938430.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            66713..67612
                     /gene="hemF"
                     /locus_tag="ACHKV2_000482"
     CDS             66713..67612
                     /gene="hemF"
                     /locus_tag="ACHKV2_000482"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO6938431.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(67618..68670)
                     /gene="eutR"
                     /locus_tag="ACHKV2_000483"
     CDS             complement(67618..68670)
                     /gene="eutR"
                     /locus_tag="ACHKV2_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO6938432.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(68716..69216)
                     /gene="eutK"
                     /locus_tag="ACHKV2_000484"
     CDS             complement(68716..69216)
                     /gene="eutK"
                     /locus_tag="ACHKV2_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO6938433.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(69229..69888)
                     /gene="eutL"
                     /locus_tag="ACHKV2_000485"
     CDS             complement(69229..69888)
                     /gene="eutL"
                     /locus_tag="ACHKV2_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO6938434.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(69898..70785)
                     /gene="eutC"
                     /locus_tag="ACHKV2_000486"
     CDS             complement(69898..70785)
                     /gene="eutC"
                     /locus_tag="ACHKV2_000486"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO6938435.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(70806..72167)
                     /gene="eutB"
                     /locus_tag="ACHKV2_000487"
     CDS             complement(70806..72167)
                     /gene="eutB"
                     /locus_tag="ACHKV2_000487"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO6938436.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(72179..73582)
                     /gene="eutA"
                     /locus_tag="ACHKV2_000488"
     CDS             complement(72179..73582)
                     /gene="eutA"
                     /locus_tag="ACHKV2_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO6938437.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(73579..74805)
                     /gene="eutH"
                     /locus_tag="ACHKV2_000489"
     CDS             complement(73579..74805)
                     /gene="eutH"
                     /locus_tag="ACHKV2_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO6938438.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(74905..76092)
                     /gene="eutG"
                     /locus_tag="ACHKV2_000490"
     CDS             complement(74905..76092)
                     /gene="eutG"
                     /locus_tag="ACHKV2_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO6938439.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(76082..76918)
                     /gene="eutJ"
                     /locus_tag="ACHKV2_000491"
     CDS             complement(76082..76918)
                     /gene="eutJ"
                     /locus_tag="ACHKV2_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO6938440.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(76929..78332)
                     /gene="eutE"
                     /locus_tag="ACHKV2_000492"
                     /pseudo
     CDS             complement(76929..78332)
                     /gene="eutE"
                     /locus_tag="ACHKV2_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(78344..78631)
                     /gene="eutN"
                     /locus_tag="ACHKV2_000493"
     CDS             complement(78344..78631)
                     /gene="eutN"
                     /locus_tag="ACHKV2_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO6938441.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(78738..79031)
                     /gene="eutM"
                     /locus_tag="ACHKV2_000494"
     CDS             complement(78738..79031)
                     /gene="eutM"
                     /locus_tag="ACHKV2_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO6938442.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(79070..80086)
                     /gene="pta"
                     /locus_tag="ACHKV2_000495"
     CDS             complement(79070..80086)
                     /gene="pta"
                     /locus_tag="ACHKV2_000495"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6938443.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(80083..80814)
                     /gene="eutT"
                     /locus_tag="ACHKV2_000496"
     CDS             complement(80083..80814)
                     /gene="eutT"
                     /locus_tag="ACHKV2_000496"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO6938444.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(80811..81512)
                     /gene="eutQ"
                     /locus_tag="ACHKV2_000497"
     CDS             complement(80811..81512)
                     /gene="eutQ"
                     /locus_tag="ACHKV2_000497"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO6938445.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(81487..81966)
                     /gene="eutP"
                     /locus_tag="ACHKV2_000498"
     CDS             complement(81487..81966)
                     /gene="eutP"
                     /locus_tag="ACHKV2_000498"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO6938446.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(81979..82314)
                     /gene="eutS"
                     /locus_tag="ACHKV2_000499"
     CDS             complement(81979..82314)
                     /gene="eutS"
                     /locus_tag="ACHKV2_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO6938447.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(82607..84886)
                     /gene="maeB"
                     /locus_tag="ACHKV2_000500"
     CDS             complement(82607..84886)
                     /gene="maeB"
                     /locus_tag="ACHKV2_000500"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO6938448.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            85175..86125
                     /gene="tal"
                     /locus_tag="ACHKV2_000501"
     CDS             85175..86125
                     /gene="tal"
                     /locus_tag="ACHKV2_000501"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6938449.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            86145..88148
                     /gene="tkt"
                     /locus_tag="ACHKV2_000502"
     CDS             86145..88148
                     /gene="tkt"
                     /locus_tag="ACHKV2_000502"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6938450.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(88243..89286)
                     /gene="ypfG"
                     /locus_tag="ACHKV2_000503"
     CDS             complement(88243..89286)
                     /gene="ypfG"
                     /locus_tag="ACHKV2_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO6938451.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(89412..89987)
                     /gene="nudK"
                     /locus_tag="ACHKV2_000504"
     CDS             complement(89412..89987)
                     /gene="nudK"
                     /locus_tag="ACHKV2_000504"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO6938452.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(90055..92034)
                     /gene="aegA"
                     /locus_tag="ACHKV2_000505"
     CDS             complement(90055..92034)
                     /gene="aegA"
                     /locus_tag="ACHKV2_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO6938453.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            92240..93940
                     /gene="narQ"
                     /locus_tag="ACHKV2_000506"
     CDS             92240..93940
                     /gene="narQ"
                     /locus_tag="ACHKV2_000506"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO6938454.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREILDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            94104..97217
                     /gene="acrD"
                     /locus_tag="ACHKV2_000507"
     CDS             94104..97217
                     /gene="acrD"
                     /locus_tag="ACHKV2_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO6938455.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGVEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(97316..97375)
                     /gene="ypfM"
                     /locus_tag="ACHKV2_000508"
     CDS             complement(97316..97375)
                     /gene="ypfM"
                     /locus_tag="ACHKV2_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO6938456.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        23813 a        25951 c        24080 g        23736 t
ORIGIN      
        1 accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca ccgagcacta
       61 acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct cttcctttaa
      121 gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt aaaaatatcc
      181 aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat atcggagcga
      241 taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt ggataattcc
      301 ttaataataa actctgtgac agtggaatta acggccctgc cattaacccc tgaacgactc
      361 taaaaaatat cagcacatca agattgatcg ataaactaca cattaaggaa gacagcgaaa
      421 aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc agcctgccgg
      481 taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag gtgccttcgt
      541 ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa atagtggaat
      601 ccaacatttg cataaacgtc gctaatgaca atgcaatagt gacgcaccat aacgtcccac
      661 cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat tatgcgaaat
      721 aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct tactggtata
      781 agccggcatg gacgtcacgg ttgaagccag ctcaggcatc tcagcaatgt cttcgttctt
      841 cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca tgagttcttt
      901 tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt tccctgtcgc
      961 attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc ctgtcacccg
     1021 gccatgaaac acaacattct caccataaag atcgctgata atattgaccg attgaccaat
     1081 ccgtacatcc gtgagttgtg tttctttaaa gttagcatta acccacattt gacgtgccgg
     1141 tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa cacttctctg
     1201 ggcaatatag ccggtaaccg gactcttaat atccgtacgt ttaagcgcca accaggcttc
     1261 tttggttgca tccgcagctt caacgacttg tggctgacgg tttaatggtg tgttcattac
     1321 taaagcttta ttcgctttat aagcctggat agcggcattc aatgccgctt tgctacttat
     1381 taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct tcgctaaagg
     1441 cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg aagcgacttc
     1501 ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa tatttgccag
     1561 attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg aaactaaaat
     1621 gtcaccctgt cgaacgtagt tcgtatcttt atgattaacg acagtgacac tacctgagac
     1681 ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac taatcatgtc
     1741 ttctaattcc attgaccaat aggcataagc acctgaaaac gcaataaata aaactatcgc
     1801 caataaagaa aagtattttc ttctgttaga atgtttttta tttgaattaa tctgttccac
     1861 tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag tattcattct
     1921 tcatatcaaa cacagtgcat gtgttgcaca ttatccctgg aacgttggag gcctaattta
     1981 aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata caattcttac
     2041 gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat catcggtata
     2101 atccctgatt ttattgttga catttcatat atgccgacta tttatatggt atacttgtcg
     2161 aattatctta aaggaagctc aaattttctt atttttattg agaaaatgag atgatgcctt
     2221 atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat aatctatgaa
     2281 cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt tattgatcaa
     2341 aaacgatatt gaaatcttag cagagttgac tgaaggcgga agtgccgttc agcgggtgga
     2401 aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta acggtatcca
     2461 ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg tctccgctaa
     2521 aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt tcgtgagtaa
     2581 aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg gctactgcta
     2641 tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc aaaaactcga
     2701 ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca aggataataa
     2761 tgacattgct gaaaaaatgt tcatcagcaa caaaactgtc agcacttata aaagtcgcct
     2821 gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac aacgtaacaa
     2881 aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt ggtctttggt
     2941 cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt agtaacaacc
     3001 gtgtcacact tgatccacta cgtctgagca acaaggaatt acgttggtta gcgagcaaaa
     3061 aaaatcttgt gattgcagta cacaagtccc aaacggctac gttgttgcat accgattcgc
     3121 agcaacgggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga gcgttaaata
     3181 tcaaattaac actccgggaa tacgcagatc atcaaaaagc aatggacgcg ctggaagaag
     3241 gtgaagtcga tatagtgtta tcacatttag ttgcttcgcc gcctcttaat gatgacattg
     3301 ctgcaaccaa accactgata attacctttc cggcgctggt caccaccctt cacgattcaa
     3361 tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat tatccccctg
     3421 acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca aatttatatc
     3481 aggcattagc atccgtctca gccggacaga atgattactt tattggtagt aacatcatta
     3541 ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa tattataact
     3601 caccgcgtca atataacttc ttcttaacca gaaaagaatc tgtcattctt aatgaagtac
     3661 tcaatcgatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca caaaattggc
     3721 ttgatacagg aaacctggcc tttctgaaca agccattaga actcactgaa catgaaaaac
     3781 agtggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcg cccccctatt
     3841 ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt aatattatta
     3901 ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc catgctggaa
     3961 cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt gaagatagag
     4021 aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt gtcatgcaaa
     4081 aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt ccatattatt
     4141 atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata aaagtcgata
     4201 acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg gtcgcgacac
     4261 agttaaattc acgttacatg atcgaccatt actatcctaa tgaactgtat cattttctta
     4321 ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta
     4381 aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg cgcctgaccg
     4441 aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat agcgagcaat
     4501 tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta tggggattct
     4561 acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat
     4621 cattccggaa agcgctctcg gactccttac cgaatccaac ttatgttgta aactggcaag
     4681 gtaatgtcat tagtcacaat agtgcatttg aacattattt cactgctgat tactacaaaa
     4741 atgcaatgtt gccattagaa aacagtgaat caccctttaa agatgttttt tctaatacgc
     4801 atgaagtcac agcagagacg aaagaaaacc gaacaatata cacacaggta tttgaaattg
     4861 ataatggcat cgagaaaaga tgcattaatc actggcatac attatgtaat ctgccagcca
     4921 gcgaacatgc tgtttatatt tgtggttggc aagatattac cgagacgcgt gatttaattc
     4981 atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa agtcagtttc
     5041 tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc ttcctggaac
     5101 ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca ctagcctacg
     5161 ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat
     5221 cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc cagaacactt
     5281 gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc agcagtacat
     5341 ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc ttatcaaatt
     5401 tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg acctccctgg
     5461 ttcacattga tgacaaccac gctgtaatca aaatgacgat tatggattct ggaagtggat
     5521 tatcacagga agaacaacaa caactgttta aacgttatag ccaaacaagt gcaggtcgtc
     5581 agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa aacatgcagg
     5641 gtgatttgtc attagaaagt catccaggca taggaacaac atttacgatc acaatcccgg
     5701 tagaaattat ccaacaagtg gcggctgtcg aggcaaaagc agaacaaccc atcacactac
     5761 ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta ttactcaaac
     5821 gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg caagcgctac
     5881 acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg ccgaatgtgg
     5941 atggttttga gttgactcgc aaactccgtg agcaaaattc gtccttaccc atctgggggc
     6001 ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aaattgcggc atgaacttat
     6061 gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag ttacaccagg
     6121 ttgcgcatat tgtacctcag tatcgccacc ttgatatcga ggccctgaaa aataatacgg
     6181 cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa acacataaag
     6241 atttacccgc tgcgtttcat gcactagaag ctggcgataa tagaactttc catcagtgta
     6301 ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt aatattagcc
     6361 atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt cagttgctaa
     6421 actctgtaaa agaacacatt gcagagctag accaggaaat tgctgttttc tgtcaacaaa
     6481 ataactaaat agtggctccc acaatgttca aatgtgggag ctatttacca gcacatctta
     6541 catttatgat gagaactcct ggcgaatttg ctccccatgc tggtcgagcg ttgccgctcc
     6601 cggcataaca tgcgggtccg cgcagccact gatttttatc ggattacccg gcatcattat
     6661 tcccccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa tggcctcagc
     6721 cacacttaat aacggcgcga cgggcacacc aacttcatgt attctggcta accaaacttc
     6781 agctgcctgt gtttttaacg tccgctcaat atattgttta agaatagtct ggttttgtac
     6841 gcgtaaaata ttgctgctaa atctgggatc attaaccagt tccgtaagct ccagtgcctg
     6901 gcataacgca gagaaaagct tgtcattacc acaacaaatc gtaatcggct tatcctgagt
     6961 atcgaaaaca tcaaaaggtg ccatgtaggg gtggcgattt cccaggcgtt gtggtgactt
     7021 ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat
     7081 cgctatatcg acatgcgccc ctctttggct cttttcgcgg ccataaagtg cactcactat
     7141 tccgctgaat aaataaacac cgccgcatag atccgcaaga gaggtaccaa cacgcactgg
     7201 cggagcatca gggtaccctg tttccatcat tatcccgctc attgcctgaa tgatggtatc
     7261 gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg aagcatatat
     7321 gaggcgcggg ttgatttctt gtaacctttc ccatgaaagc cccagttttt ccattgtacc
     7381 tgggcgaaaa ttctcagcta atacatcagc ttgtttaagc atatttataa atatactttt
     7441 atcgtgatca ttctttaaat caagaaccac actctcttta ccatgattaa taaaactgta
     7501 atagagtgtc catccacata gggaccaaat gtgcgggtat catcaccatg acccggcggc
     7561 tcaactttaa ttacccttgc gcccatatta caaagaagtt gagttccgaa aggtccatta
     7621 aggacatgtg tcatatcgat aactaataag ccttcaaacg gccctttgct ttcattattt
     7681 gtcatttgct tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa
     7741 aatcaataac atgattaatt atgaaaccag tcgactgaca taaatccaca agggagccgt
     7801 gacgacaaaa cccagtacgc tcacagccaa tgacgctgta ccagtgcggg tgtagacatt
     7861 aaaccggctg gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat
     7921 catctgcaga tgttcgctgt tcaaatgaca tgccatacca acaagaagca gcgccagtgg
     7981 catcagaatc agcttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg
     8041 tgcagccaga gtcaaccccg cagcgaatac cgctacccct gagttagcct tcgcaatcag
     8101 attaaaggtt gggtcccatg ctgccggaat ttttacccca accaacacca ggatcgttgc
     8161 cagaacaggt gcccatacaa ctggctcctt tgccgcagaa attaatgcgc tcagattact
     8221 attcttctta ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt
     8281 aattgcgtta acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc
     8341 ataaatagga tcgagaactg caaaccccag gaatccaatg gtaggtgaac ctgcaattaa
     8401 tgcacataca gctgcttctg catgggtacg tttaaaaaat ttgtagcaac caaaccagga
     8461 gaaaaagaaa catccgacaa taaccacgag tgatacaagg gtcagacgag tgtccgcaaa
     8521 aatcatttcc ctgtttgccc gggtaataga aacaaatagg gctgcaggaa gcgcgtagtt
     8581 taataccagt ttattaaatg cccgggcttg atcttctgaa aaagtttctc gtctgccgct
     8641 aaaataaccc aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag
     8701 cataatagta tttccgataa gggtcccctc accatcgcat aatgagttgg caagaggaga
     8761 cgatgtcagt atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact
     8821 ttcagttcct gctgcagggt cgatgaccac attaataatg gtcggtttgc gcgactgaat
     8881 accggtggtt aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg
     8941 aaacgcatcc attaatttgt cataccttgc atggtgcaac agatctgttg gtgatggtgc
     9001 accagcgcca ctgagatcaa caccgtctcc tctgtagatg ccgccattat taaaaataac
     9061 gatcgttacc ggcaggttat atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa
     9121 agcactatca ccttcaatgg cgacaaccgg agaaccagag gtcacgctag caccgatggc
     9181 atagcccata ccgatgccca tgacacccca ggtaccacaa tccagacgac gacgtggttt
     9241 atacatatca ataatatttc gtgcattatc cagggtattt gcaccttcat taactaaata
     9301 aatatcctgg ttctcacgca atacatcgcg cacagcactt aatgcattaa agtaatttaa
     9361 tggttgggta tctgtactta atttttcatg cattttttgt gcattttgct gcttgtggat
     9421 atttaaaata tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca gctctgccag
     9481 cataccttgc atactggatg caatatcgcc aacgactggc acagcaatgg ggcggttgct
     9541 gtcaatttcc tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt
     9601 tttaccgtgt gccagtaacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt
     9661 tgccagggca aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt cgaggatccc
     9721 tttcgccata gacattggca ggaatggaat ctgggcactt tcaataaatt cacgaagctg
     9781 ttcgtcagct tgtgaatacg ccgcgccttt gccaaggata attaatggcc gttcagcttt
     9841 tgctaaaagc gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg
     9901 attttcaact tttacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac
     9961 atttgctggc aaatcaagat aaactccgcc agggcgaccc gatactgaga cccggatagc
    10021 gcgtgccaat gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata
    10081 cggttttgcc gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat
    10141 cgcgcggtcg ctggagccgc taatcatgat catcggaaaa ccatttaccg ttgcgttggc
    10201 caatgcggtc aaaccattga ggaatcctgg cgcagacact gtcaggcaga tccctggttt
    10261 ttgggtaaga aaaccgcttg ccgcagcggc atagcctgcc gactgctcat gacgaaaacc
    10321 aatataacga atgccttccg cctgggcatg gcgcgccata tccgtcacag gaatacctac
    10381 aacaccataa atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc
    10441 atctgtcatt tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga
    10501 gccagaagcc cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg
    10561 tcgctataac ccagctcctg caataccgca gcggtatgtt cacctaatag aggcgcagct
    10621 ttaatatccg gcgtaaaggc agagaatttc attggacaac caactgtcag atattttcca
    10681 cgcaatggtt gttccacttc gacaacactg ccgctttggc gtaaagaggg atcaagtgaa
    10741 atttctttca tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataagccact
    10801 gcttcatgtt tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata
    10861 tgtggctgtc gggcatgggc tgtactgtat gccggatcgg taatccaatc tggtttgccg
    10921 atggctttac aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta
    10981 ggatcagttt cccagccttt acatttcagg atccagccag gctgaccgcc accacccgca
    11041 ttacctccgc ggggaactgc atcaccaaat gtgccattcg gatactgcgg gtattcttcc
    11101 agataaccca atttatcgag acgctgctgg tcgcgtaatt tcacgcggca aaggttcaat
    11161 acggcatcct gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc
    11221 aaagcagcaa gtaaaccgat cagtaaatgc attccggtgt tgctgtcacc caacgctgca
    11281 gcgcttacca gcggcggacc gtcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc
    11341 tgagcaacgt tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg
    11401 atcgaaccaa aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccatg
    11461 tgatcaatgg cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt
    11521 tccattacct ctttgccttc cgctgttttg gtatttaact caatagaacg tttgttactg
    11581 ttaagcatgg tgaagtaaag cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt
    11641 acgtcaccaa cgccggggcg ttcaatttta atgacgtcag cgccaaacca ggccagcatt
    11701 tgagtacaag atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt
    11761 ggagttgaca tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta
    11821 aggcataacg ccctctataa ataatccgca aaatatcgtc atagcggcac tcgatatttt
    11881 taaattcctg tatcaagtgc tgaaaatcat ttcatcgcta cttccaattc ctttctgcat
    11941 ggtaagtagt gaggacgaaa agaagaatat gataaatatt ggttcgtggt aatcagagag
    12001 agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta
    12061 agggtatgat tacgcttttt agagcgtacc ataaaatata acatattaaa tgatttgtga
    12121 tgacacggga ttattgcggt ttactttgag gaatataaaa tgcgagttta tagaacagcg
    12181 agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct
    12241 ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg
    12301 cttcgctatc gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt
    12361 attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg
    12421 gcatcagata ttggttttcc attacaccaa caccggccag gcgaagctct tccattgcgc
    12481 cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg
    12541 gtgtcgctac ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat
    12601 cattgacatt ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg
    12661 acaacaaagc tgaagcttca ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca
    12721 gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct
    12781 ctgctgcagc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    12841 ccggagcatt atcagcagcc catacagttg aagatgccag aatagctgtg accatcgtag
    12901 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    12961 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    13021 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatatgcttc
    13081 ctgtcacaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    13141 atattgatta attatatgta aatacagcca taacatcgta cattctcagt gaaccatttt
    13201 caggataact ttctatgaaa gtaaacttaa tactttttag cttattttta ttggtctcta
    13261 ttatggcatg caatgttttt gcattttcta attctggtgg tggtagtgag acgagctata
    13321 aagagccaga aaaaacgtca gcgatgacga caactcactc tacaaaattt cagccatcac
    13381 aggcgatttt gtttaagatg agagaagatg caccaccatt aaacctcaca gaagaaacca
    13441 cgcccccttt tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    13501 ctggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gaaccatcct
    13561 gacccactat ctcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    13621 aagaaacata aatatccccg tcaccagttc attaccgcca gttaaccaca tggtgaagta
    13681 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    13741 cagtacctga ccgatgtatc cgcttacgca gacaatacac agggcgagaa acatccataa
    13801 ataaaacata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    13861 gtaagacaat ccttaacctc tggcaatttt tgctaagcgt caatgaaaac agcatgttaa
    13921 atgcaatact attgcgtgcg gaaaattttt tactttgcac gattaataat cagtagctga
    13981 aagcagtcag ccagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    14041 gactagtctt tacactcttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    14101 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    14161 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    14221 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    14281 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cggcgtgaat ctatcgcccg
    14341 taaaaacctg gaactttgct tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    14401 cgaaaacttt cgttcactcg gcatggctct ggtagaaacc ggcatggcgt ggttctggcc
    14461 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggctg gataacctta aacgcgcaca
    14521 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    14581 ccgcgtgatg ggactttgtc agccaatgat ggctacctat cgtccacata ataatcagct
    14641 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    14701 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    14761 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    14821 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    14881 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    14941 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    15001 catgcgcgca ccggagcagt acctctggat ccaccgtcgc tttaaaacgc gcccggtggg
    15061 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    15121 aataacctca tggtttttat ggcttttttc ttaagatttt ttccttcgct gaatatctaa
    15181 ttattaccgc cagaaattaa actgtcgccg atatgagaca cctgtaagtt actaaaagta
    15241 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    15301 agtttagctc agaattctgg ggtgatgccc acggtaaaat acaggacttt taaggtcgtt
    15361 ttgcccggtg tcaactcact gtatcaggtg tgatgaagtc attcaggcgt aaccgtaatt
    15421 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    15481 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    15541 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    15601 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatcccac gaatcgcctg
    15661 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtgtcgc cgtagtcacc
    15721 aaagccaatc cctggagaga cacagacctt cgcttcgtta aggagcttct tggcaaattc
    15781 cagcgatccc atggccgcat atggttccgg gattttcgcc cagacataca tcgaagcctt
    15841 cggcatttcg accatccagc ccgcttcatg cagcccttta accaatacat cgcggcggcg
    15901 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    15961 cgccacctgc aacggcgtaa aagtgccgta atcgtgatag cttttgattc gcgccagggc
    16021 actgaccagc gttttgttgc cgaccataaa gccgatacgc cagcccgcca tgttgtagct
    16081 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    16141 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcatgga ccaccagcac
    16201 atcgtagcgt ttcgccagcg ccactacttt ttcgaagaac tccagttcca cgcattgcgc
    16261 ggttgggttt gacgggaagc cgaggatcat catcttcggt ttcggataac tttcaagaat
    16321 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    16381 cgccccggca atcaccgcgc cataaatatg gatcgggtag cttggattcg gcaccagcac
    16441 cgtgtcacca tgatccagcg tcgccagcat caggtgtgcc agcccttctt tcgaaccaat
    16501 ggtgacgatg gcttctgatt ccggatcgat ttcaacgtcg tagcgatcct gataccagcg
    16561 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtccgg
    16621 acgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    16681 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    16741 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    16801 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    16861 cacgatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    16921 catttttatt aatgacgtta aatgaggaac tctgctttaa aaacaggaaa tttatctgcg
    16981 ctaacaagaa aattaccact cattttatta acataataat ttcaataaat ttactaacca
    17041 gaagtgcatt atcaagagat gcattgctaa tacaccattt atctatcaat ccccttttaa
    17101 aattgtggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    17161 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    17221 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    17281 tcgcgaactg ttgcacaagt cggcacactc accgaaagag ttgctcgccg ttaccgccat
    17341 tttctcgctg ttcgccctct tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    17401 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtgggcat
    17461 cattaccggc gtgattgcgg gtattcatcg ttatttaatt gatatcggtg gcgtgacggc
    17521 gatcccctgc tttatcacca gcattctggc tggttgtata tcgggctgga tcaacctgaa
    17581 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgaaacgtt
    17641 gaccatgatt ctggtgattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    17701 taaaatcggg attccaatga tcctcggcag cgtctgtatc ggctttattg tgcttctagt
    17761 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagt tggcgctgga
    17821 tatcgccaac aaaacgctac ctctgtttcg ccatgtcaat agcgagtcat tacgcaaagt
    17881 ttgtgaaatt attcgcgatg acattcacgc cgatgcggtg gcgattacca ataccgatca
    17941 tgtgctggcc tatgttggtg tgggtgaaca taactatcag aatggcgatg acttcattag
    18001 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    18061 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    18121 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    18181 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    18241 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    18301 tttcctgttt aacgctttga acgctatttc atcgtcgatc cgtctgaatc cggataccgc
    18361 tcgccagctg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    18421 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    18481 ggcccgcttt ggcgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    18541 cattcccagc ctgctgatcc agccgttggt ggagaatgcc attgtccacg gtatccagcc
    18601 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggcaatc gggtacgcat
    18661 tgcggtgcgg gataccgggc acggcatcga tccaaaggtg attgagcggg tcgaagcgaa
    18721 tgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta
    18781 tggcgagggg ctgcatatcc gccgcctgga accaggaacg gaaattgcgt tttacattcc
    18841 taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt
    18901 gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt
    18961 aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag
    19021 tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat
    19081 ggtgtgttgc tggcacaaaa catcagccag ttcgcccata aaccgtttat tgtgttcatc
    19141 accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga ctacattcta
    19201 aaaccgtatc aggagtcacg cattaccggg atgctgcaaa aactggaagc agcctggcaa
    19261 caacagcaga ccagcagcac gcctgccgcg acggtaacgc gtgagaatga caccattaat
    19321 ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta cgccgaagcg
    19381 cacgagaaaa tgacctttgt ttatacgcgg cgtgaatcct acgtaatgcc gatgaacatt
    19441 accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg ctcattttgt
    19501 gtcaatctga acaaaatccg cgaaatcgaa ccgtggttta ataacaccta tattctgcgg
    19561 ctgaaagatc tggattttga agtgccggtc agccgcagca aagttaaaga attccgccag
    19621 ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa
    19681 tttttcgccg atctgttcag cggcctggtg cttaacccgc aactactggg gcgcgtctgg
    19741 tttgccagcc agcctgcctc gttgccggtg ggcagtttat gtattgattt tccccgtctg
    19801 gatatcgtgc tgcgcggcga atacggcaat ctgctggaag caaagcagca acgtttggtg
    19861 gaaggagaaa tgctgtttat tccggcgcgc gcggctaatt taccggtcaa caacaaaccg
    19921 gtgatgctgt taagcctggt gttcgctccg acctggcttg ggttatcgtt ttacgatagc
    19981 cgcaccacgt cattgttgca tcctgctcgc cagatccagc ttcccagcct gcaacgcggt
    20041 gaaggtgaag cgatgcttac cgccctcacc catcttagcc gttcgccgct ggagcaaaat
    20101 atcattcagc cgctggtgtt aagtttgctg catctttgcc gtaacgtggt gaatatgccg
    20161 ccgggcaatt cgcagccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag
    20221 gataattatg cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc
    20281 aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt
    20341 tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatttgtc aattcatgaa
    20401 gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag
    20461 tttggtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat
    20521 tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg
    20581 aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct
    20641 tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag
    20701 tcaaccggtt tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg
    20761 gtgtgcggga tcgccacgcc aaaaccaacg ccagtggtga caatctcttc ccgctgccag
    20821 acatcctctt ccagctcgaa cggatgttca gtgcgcccgt taacgccgag gttaccgcac
    20881 aggaactgga tcgcctgctc tttattgcta aaggcctgat caacgaagat attctccagc
    20941 gccagcagcg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc
    21001 tgcgcgctgc gacattcaca cgcctgacgt gccagttccc gacacgcctc gctatccagt
    21061 tgacgaagct ggcttttcac cgccggaatg cgcgggctac tcatactcag ctcatccagg
    21121 cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct
    21181 acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg caggaacgat
    21241 ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac cgcatacaga
    21301 tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtgatcg
    21361 atgatgtagc acaccgacgg cacttccacc atgataccca gcgtaatcgt ctctgcatga
    21421 cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccctt cacccataag
    21481 atctgatcga ggccgtggac catcgggatc atcaactggg cgttgccgaa actggcggcg
    21541 cgcaaaatgg cccgcagttg agtgcggaac aggccagcaa attccgggta aatacgtacc
    21601 gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta
    21661 tcgcctccga tgtccattgt gcggaagata atcggcttgt cgcccgctgc cagtagcacc
    21721 tgctggtagg cttcaaattg ctcctgctcg tccggctcgc tgtcgcgatc catatagagc
    21781 atttcggtac ggaacagccc gacgccttcc gcgccgttgg caaacgcgcc aggcgcttcc
    21841 agagcggtgc caatattcgc cgcgatatca atacgcttgt tatcacggga ataagccagc
    21901 tgcgcggcag cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg cgcgacctga
    21961 taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg ggcgtcaagc
    22021 actgcaggtt gcccggcata acgcgcgatc gcatccagcg gcaggccgct cagtaccggg
    22081 atcgccgagg cgcgggccag aatcagggta tgcgaggtgc gcccggtttt ctccagaatc
    22141 atgcccgcaa gattttttaa atccaggctt aaaaactggc ttggggttaa atcctcggcc
    22201 accagaatag tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc cggccaggta
    22261 atatgcagca actgttcgct aatatcgcga atgtcgctaa cacgttcgcg cagataatcg
    22321 ctggcagaag cagaaagttt ggcgcaaacc tgctccatat ttctgatgat cgccgccccc
    22381 agcccctgat gctgttctgt catcaggcga cggatattgc ctgcaaattc atcatcctga
    22441 atcagcgaca aatgggcgct gaggatagtt ttgctttcgc cgtcacgctc acgcaattgc
    22501 tggttcagtt gctcggcaag cgttgccagg ctgtgctcca gccgggtgga atcttgcgca
    22561 ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc
    22621 acgccgacgc cgcttgccag cacattgccg tacagtaaat ccgggttaag gcggcttaat
    22681 gaacgcggca gcggatgcgc cgtcagttct gcctgcgtag gctgaacgct gtcgctgtcg
    22741 ataaagcgca cctggatgta ctcttccagc acgcgccgcg cctgctcttc atcgctgccg
    22801 ctaatgttca ggctgcaact gtcattaaat agggtgccgg tgccaatcag cgccagcgag
    22861 cttttggcat cggcctttgc gttctggcga tggttaataa aagtgatttc gctttgccac
    22921 tggctgcact gttctttaag ttcccacgcc ggacgagcat gtagaccgtt aggcaaagga
    22981 cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc
    23041 gtcagttgaa caaccgtctc acgcgtaaga cgttgaataa gggcagataa aagttgctcc
    23101 atctgcaaaa tgtcacggca atcggcaatc gacgcggcgc aatgtccgtg gcgggtggca
    23161 ggccccatca ccactgtggg tacgccagta ccggttaagt gcaccgctcc accgtccgtg
    23221 ccgccgttac tgaacatatc cgcctgtaac ggcacgccaa tttctgccgc cacggtttcg
    23281 atccaggcgg tgagttttgg cggcgcaatc agtgacttgt cgcttaacac cagcatcggg
    23341 ccgttaccaa tctggcgatg gttagccgcg ccataattaa agtttttcgc ccagcaggcg
    23401 gtatcaagga caatggcgac gtccggcgac accgcgcggg tggcagtttg cccgccgcgt
    23461 aaccccacct cttcgctgga actggccacc agccacactt ccgcaggcag ttcagcgctg
    23521 tgtagttcgc gcagtaacgt caccagcagg tagcaaccga ggcggtcatc aaaggctttc
    23581 cccatcaccc gctggtgggg gagaacctga aaagtggtat cgaacgtgac gcgatcgcct
    23641 ggacgaattc ccgcctgcat cacttcgtca tacgagcgcg cgccaatatc cacgcgcatg
    23701 gcgctgacat cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca
    23761 cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgtacatt gccaaccggc
    23821 agcacatcaa tcgccccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata
    23881 tgcgcacaga tcatcacctt cggacctgtc gattcattga ggcggatcag caccgatccc
    23941 agcccatcaa atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc
    24001 acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgctttcaa tagcgataaa
    24061 tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc
    24121 cttgcggggt gaccagcaca acatcctcga tgcgcacgcc cccttgccct ggcaaataaa
    24181 tccccggctc cacggtcagt aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa
    24241 aacgcggatc ttcatgaact tcaatgccga tagcatgagc ggtgttatga ccgaaatagt
    24301 ggctaaaacc tgcctcggta atcacccgac gcgcggcttc gtcaacctgc tggcagcgca
    24361 cgccggggcg aattgcagag attgctgcga gctgtgcctg caaaacaatc tgatagacgt
    24421 taaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca
    24481 tatcagagca gtagccctga tagagcgcac cgaaatcgag agtgacaaac tcgcccgctg
    24541 caacaatctt gtcgctggct ttgccgtgcg gcagcgcccc acgccagcca ctggcgacaa
    24601 tggtgtcaaa agaggttttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg
    24661 ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctct gcaccgcgat
    24721 cggcaatccc acaggcaagg cggattttct ccacctcctc tggcgttttg atttgccgca
    24781 gcacatccgg cgtggcgcta accagtttcg cattgagttc agactgccag cgatgcgcgg
    24841 tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga
    24901 tttgcctggc gatggttgta agcgtgtgcg tcgcgtcaag caaatgcagc tggtaacctt
    24961 gcgtgcgggc ttctacatcc gcgtaatagc gcgagtccac cagaatgtgc gcactttcac
    25021 ggctaatcag cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc
    25081 gtgaggagag aagcaccgca tccagttgtt gcgccttaag ccagtcgcgc agcgaagcga
    25141 gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa
    25201 catcatcaga cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc
    25261 cgccatatag acgcccaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa
    25321 ccattgcact gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata
    25381 cgacggaatg gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactgatccc
    25441 cataaaggca aggaacatcg cggtcttacc ctgcggataa agttgtgcgt tgaacaggcg
    25501 tttgccggtt aaacgacggt caataatggt cgccagaccc agaccaatcg gcggaataac
    25561 gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac
    25621 aaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc
    25681 cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacagtac ggataccgcc
    25741 gttgatccag cccccaaagg gggtgatgac gtagtacatc gccagcatga caaaaatagc
    25801 ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat
    25861 tttctggttc atccatttca caaggtaacc ggcaacgata gaaataatca gcgcaccaat
    25921 gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag
    25981 cagttgcgtc ggctgggtag acatcaagcc gccgataaaa ccagccggaa acgccagttt
    26041 gccaccgata gagttcgcca caaaagcagc aaacatcgga atggcaaaac caaacaacac
    26101 gccgccgaag gactgcgaca accaggcaaa tttcagtaag gagaggtcga aaccggagaa
    26161 tttcccgcta ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata
    26221 agcaatcaac tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg
    26281 tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg
    26341 cggattctta actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt
    26401 tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc
    26461 ttcgatttct ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata
    26521 aacgtcgcgt gattcgaaac gttcgttatc ttccggggta actgccacgg agtggatgat
    26581 gatggtcgct tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg
    26641 ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccagcg cctgagcggc
    26701 cataaaagtg tgagccaggc ccattgggca ggcacataag gcaatcagtt tcttactcat
    26761 cgttaattcc tcgttcagtg atagttgacg agtgaaatta tgaacgtgca aaacaaatcg
    26821 ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac
    26881 gcaggtcggc cttgtgaggg gacacaaaga aaagttgaat taactgtatg atttaaaaga
    26941 ttatcgggag agttacctcc cgatataaca ggaaggattt acagaatgtg acctaaggtc
    27001 tggcgtaaat gtgcaccgga accgagaaga cccggattgt catggacgat gagatacacc
    27061 ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcggc acggaaacca
    27121 gaggctttga agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca
    27181 aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc
    27241 gacaacgcac ggcggcaatc ggtgcagctg tcagccagcg cgcgttcggt aatatctttt
    27301 ggcttgagat tttctggcag gcggttgtcg gctttaacaa ttgcgcgata caaattcacc
    27361 agtcctgggc cagaaagcac gcgttccgcc gaaacatgac caatttccgc acgcaatatt
    27421 tcgaggataa tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc
    27481 ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag ccccgttccg
    27541 gcaccgtaaa ccgcaatagg cttaccttct accggttctg cgccaccaaa ctgaatcaga
    27601 tgctcttttt tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc
    27661 agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg
    27721 gtcattgcca cccagtctcc ggtaatcggg caagcgatgg caatacagcc gtctttcacc
    27781 tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc
    27841 cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt
    27901 gcgttggtgc cgcccacatc accgactaat gcatactttg tcattcttca actgctccgc
    27961 taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg
    28021 aaaggcaggc tccctgtaaa tatcgatctg ggtcacacaa ttactttatc gtttcagcac
    28081 caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga
    28141 atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc
    28201 aattgggatt gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca
    28261 gaatttgctc tggcaacgac tgccgggaac tctggggata gcccaggatt cacccctctg
    28321 gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca
    28381 gggtcatgcc ggaccagacc ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc
    28441 tgcgctacca ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg
    28501 gccggaacat ccgatcatga ccgtcaatat cgcccttgcg gttgccattg gcgctcgtct
    28561 gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc
    28621 gctgtttggt acacctgttg cggcggcgtt gatattttcg caaaccttaa atggcagtag
    28681 tgaagttccg ctatgggatc gtctttttgc gccgttaatg gcggcagcag ctggtgcact
    28741 tactaccgga ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat
    28801 ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcagggat
    28861 ggtcgccgta tggtgcttac cacggttgca cgcgatgatg catcaaatga aaaatccggt
    28921 gctcgtgctg ggtattggcg gatttattct cggtattctg ggggttattg gtggaccagt
    28981 ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac
    29041 cagcgattac tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag
    29101 tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat
    29161 gctgcatgag cacgttcccg ccgtaccagc ggcaataacc gtttcctgcg ctattctcgg
    29221 catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg cagtcgttgt
    29281 acccaatacc acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt
    29341 agcaggtaag ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataacgt
    29401 ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg gggctttacc
    29461 tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat
    29521 gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaacgc
    29581 aggcgaaatc accctactgc catcaatcaa attacaaatt ggcgatcgcg atcattacgg
    29641 taattactgg gacggtggtc actggcgcga ccgtgactac tggcatcgca attatgaatg
    29701 gcgtaaaaac cgctggtggc gtcatgataa tggctaccat cgtggctggg ataagcgtaa
    29761 agcatatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg
    29821 ccgcggacat ggacaccgcc attaaacaca ttcgccaatg gagcacaagg cctgatgcga
    29881 cgctagtgcg tcttatcagg cctacaaacc ggctctttaa cgcgtagggc ggataaggcg
    29941 tttacgccgc atcgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    30001 gtccccacca ataaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    30061 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    30121 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    30181 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta
    30241 cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc
    30301 agtgtcccca ccaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    30361 gccgcagcat ggcttaacag cggttgcagc gtcaaataag cctcatcaag atcggcaaca
    30421 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    30481 aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    30541 cgcgaaccgc catgtaaatg ctgagtgagc gaagagtgca aataaatcac atgcggcata
    30601 atcgtcgccc ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg taaactcggg
    30661 atcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    30721 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgg tttttgcccg
    30781 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    30841 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    30901 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    30961 tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    31021 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    31081 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    31141 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    31201 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    31261 gctatgccaa acgagaatga ttatcaaatt catttaaatg ggttgtggtg atttctctga
    31321 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    31381 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagct
    31441 cactattttt gaagcttgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    31501 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    31561 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgatcctcg cactgctggt
    31621 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    31681 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    31741 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    31801 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    31861 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat
    31921 tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag
    31981 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc ttggcaaaat
    32041 ctcccgtaac cgtatgtaca ccatggcagc aacggcgatg tccaccgttt ctatgtccat
    32101 cgttggtgca tacatgacca tgctggaacc aaaatacgtc gttgcagcgc tggtactgaa
    32161 catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgcctgtga
    32221 agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    32281 atacattctg gcaggtttca aagttgccat tatcgttgcc gcaatgctga ttggctttat
    32341 cgccctgatc gccgcgctga acgcactgtt tgctaccgtg actggctgga ttggctacag
    32401 catctccttc cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt
    32461 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    32521 gttcgttgcg atgatggatc ttcagaaaat tgcctctaca ctctctccgc gtgctgaagg
    32581 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    32641 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaagct
    32701 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    32761 agaccataca taaaaaagcc ggggataatt ccccggcttt tttacgcctg ttaatcagct
    32821 aatggctgcg ggcgaccaat caaataccct tgcagatatt gcaccccgag cttatgcaat
    32881 agcgcctgtt gctgttgcgt ctcgacaaac tccgcgacca cactcaatga cttcgctttc
    32941 gccagatcgg taattgatct cacaatcatc gcatccagcg tattcgtgac aatatctttc
    33001 acaaagacgc catcaatttt gatgatatca gcctgcaaac gctttaaccg ttcgtagttg
    33061 gcatagccgg tgccaaagtc atcaatcgca atccggaaac caaacttatg cagctgctcg
    33121 atgttgtaca tgctgctttc tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg
    33181 accgcctgcg gggagatgtg ataacgctta aatagacgaa taatccgccc ggcaatattc
    33241 ttttgcagca gcgtgagcgg cattaaatta actgaaaagc gcgggccttt tttgtcgcaa
    33301 gggtgtgttg ccagccactt caacaaggat tccagcactt gcaaatcaaa acgcgcgcta
    33361 aggttaaact gagcaataag gggcagaaac ttatccgggg tcataatgcc gccgtcatat
    33421 ttcagtcgcg cgaggacctc atcataacct tcaccctctt tgttgcgaat tggctgggcg
    33481 tagagcagca aatcaccctg atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt
    33541 ttagtggtct gcccggaaac catctcctct cggctatcca gcgccagcac atgatgatgt
    33601 gcgcaggatt gctccgccag ccagcttaac tgccccaaca agggttgcag ggtttcctga
    33661 tttccatcaa aacgccccca ggcagcacca tagcccatat ccagcccggt attgttccag
    33721 tgaatttgcc gactattcag gatgttaacc atatgctgga gtcgcccttc cgtttcaggc
    33781 ccgctcagca ctaacagcag ttcactaccc ggcaattgat acaacttttc gttttcctgc
    33841 atcaatggca gcagcgtacg gtaaattgat cggatacagt gaacacgcat catcaacccg
    33901 taatgacgac tcataaactc aagattatca atgcgcaggc agcaaaaact cttgcccgcc
    33961 tcttgctccg gcgcttgttc caacgcacga aagttgggca ggagcgttaa cggatcggtc
    34021 agcgcctgca aatgccagcg gcgattaagc cattcactgc gatgataaat gcgcaccata
    34081 tagagcaggc aaacgctaaa ggaaatcagc accgcgagaa taaatgccag cgaatattcg
    34141 gtttccaccc cttgcaaaaa gttctggttg taattcagca ggcaaagcgt tgaaacagcc
    34201 caagtgagat ttaaaaacgg atagcgaagc ttaccgaccc cgagggtaaa gatgatgaag
    34261 aaaacgggta ccaggtaacc ggcaataaag tcgttttcat aaggtgtgca caacagaagt
    34321 agcagcacgc ttaacgctgc cagccagctt aaggtaaatg cgcgtttctc tttgcccaac
    34381 gacggagcga tatccctgcg ccacaatatc tgcgcaaagt ggggacttac aatcatgcga
    34441 gtgagatagt agaaaagcat gttgtaaatc agcacagcgg tgaaaaggct tagcaaatca
    34501 acgaccgtga aaatggcatc cgcatcgccg aaaaaggtgg atatctttag cgggaaatca
    34561 aagaagctcc ccacaagata catgctgcat ttgatgccga tcggcgtcac caaaccaagc
    34621 cagaacagac gttgccagac atatcggctg gtcaatccat aacgccaacg cgtccccagc
    34681 tgccagcgta atattgcgca ggcacacaac acgacaaacg tctggctacc gagcatgacc
    34741 gcagtctgta aaaaagagag atgaaagttc cacagattgg tgcaaaacat cccgcataaa
    34801 acgggaacga caccgcgcca gccaaagata aacaagattg acagcattac gcacagtggc
    34861 atccacgcca gaaagatata actggaatgg ataatggcaa gtggcgaaat aaaacgggat
    34921 agctgaataa gtaccacggt gagcgtaaac gctagtgtga atatcttgat attttttatc
    34981 aggttatgct ccacaaacat gagtacacta ctctcactga tcggggtttt caggcacaca
    35041 aataataagt ttattcgcat tgcgaagcaa acaggggcca tcctgttgcc gatttttcat
    35101 ccaatgatgc tttcaccagg cggcactaac ccggacgtag actgtattac aaaagcggca
    35161 aaaagcagag acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg tggagctaag
    35221 cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc
    35281 cccacgatgc gtttacgtac caaatttgct gggtgcaaaa tttggtggag ctaagcggga
    35341 tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccgt
    35401 cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga atgtgtcaac
    35461 ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac aattttggaa
    35521 aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt gctataaaat
    35581 gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag aggatgacac
    35641 cagatgaact tgccagactg accggctata gccgccagac cattaataaa tgggtacgca
    35701 aggaaggctg gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc cgactggttc
    35761 acgtcaatga acaggttcgt gaatatattc gtaatgctga acgtccagaa ggtcagggag
    35821 aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt gctggtgaca ctggcgaaag
    35881 agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa gggattatcg
    35941 gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt acgcaataaa
    36001 atgaccaccg aagaactggc tgaatgcctc ggtgtggcta aacaaactgt taaccgttgg
    36061 atcagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg tcgtgccaga
    36121 cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc cttccataac
    36181 acaccgatgc ttctggaagc cgaagaaccc cttgccgaat acgcaccggg gattcgcact
    36241 cctgcttatc ggcaaatcat cagtgcaata gacaacatga ctcacagtga gcaggaaaaa
    36301 gttgcgcaat ttttgtcacg cgaaggaatt cgtaatttcc ttgctcgtct cgacatagat
    36361 gaatcagctt gacatcctcc acgccctgaa tgacgaggat ccctgctacg ttcaggctgt
    36421 cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatctctcc
    36481 acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccgttta catcggcgtt
    36541 cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcggt tttctttcgc
    36601 tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag ccagtacctg
    36661 accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc agccctgatc
    36721 cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt
    36781 acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattgcgtg
    36841 gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcggtaca
    36901 ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttattgctga atttgacctt
    36961 gcggcttaac tgtcgctgaa gtctcgccaa cttcttctgg tttttctgaa aactgtttac
    37021 aggctcaaag actgtgccat ctgacagcgt ggcgagttta gccacgccag catccagtcc
    37081 gaccattgat gctgaagggt gaaccggagt tgatacttca ctttctgtct gaatactgat
    37141 gtaccactta ccgcaggact ggctgacagt gacatttttc acaacacccg tgacctgacg
    37201 gctgttcagg tagcgcatcc agccaagttt cggcagaaaa atacggctgt tttcctgatc
    37261 gagcttaacg ccctgcgggt agcggaatgc atcattctgt ccccgctttt tgaatcgggg
    37321 aaaagcagcc cgcttccgga agaagttttt gtaagcccgc tcaagctctt tcagtgactg
    37381 ttgcaatggc tgtgacgggg catctttaag ccattgcgtt tcagtggcgt ttttccactc
    37441 aaccagccag gaagccattt tcccgtaagg gatgtattta ttaccggcct catgattctc
    37501 attctgaagc gccagagcac gattgaaaac gaaacgacat gcgcctgcga agcgcctcat
    37561 ttcacgctcc tgttgaccac ctggtcttaa ctggaattta aatgcctgta gtcgtttcac
    37621 aggccaaatg atactttggt ctatgaataa agaaaccgat atccgacggg gaaggcattg
    37681 tgttttcctg atgcatgttc atctgttctt tgtcaccaga taccgacgtc agatttttga
    37741 ctatgacgcg acagaaaaac tacgcactta cttttcaaat gtatgtgctg attttgaagc
    37801 tgaactggtt gaaatggatg gcgaaccaga tcacgtccat ttgttaacta actatcctcc
    37861 caaactggcg atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg
    37921 acgagatcga ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg
    37981 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
    38041 acagcaaaca cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggacgggg
    38101 ttttacggcg caccggataa ataaaaaacg gcaggggtat ctctcctgcc gtttatcttt
    38161 tatgcgctaa ttactgctga ttttcgcgtt ccgcaataaa agccagcgct ttgttgatac
    38221 gctcgatact gcgggtctta ccgatcgcgt gaacggtaac gtccagcgcc ggagactgcc
    38281 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    38341 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc tgtccagtca gtaatcgcgg
    38401 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    38461 cggcgtcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    38521 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    38581 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    38641 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    38701 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    38761 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    38821 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    38881 tgccgtcatc gccgttgatc atagaaacgt gcgcgtaaac cggtaccggc gctttcaggg
    38941 ctttaagaat gttgatctgg cgtggcgtgt tgttgatatg gtcttcgcca cggataacgt
    39001 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    39061 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    39121 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtacg acacacggct
    39181 catcatcagc gtgatgctca tgactgtggc ggcagcgacc gtcataacgc ggcttctcac
    39241 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    39301 aagctgtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    39361 tctggaagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    39421 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    39481 caccgccgtg attacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    39541 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    39601 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    39661 ctcccgcctg attcctcaat gtagttcggg ttgcaacctt acgcattatt gttttcgttg
    39721 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    39781 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    39841 gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    39901 gatcccgtca tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag
    39961 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    40021 gatcccgtca tcacccacca ctttctcacc agctaaattt cttgtaaaaa tgtgaagtac
    40081 cgaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    40141 tcgatcccgt catcacccac cacttcgggt cgttagctca gttggtagag cagttgactt
    40201 ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caatgtaaaa aagcgcccta
    40261 aaggcgcttt tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt
    40321 cttctgccga tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa
    40381 cttcaattcc ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg
    40441 cttcacaaat cgcgcggata agtcagcaaa tcatcaaact cactcaacac attaaagaga
    40501 ttgtagacac tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc
    40561 aaatcaccct actggaaaca caactggcgc agttgcagag acagcaggca gaaaaggcgc
    40621 aggagaaaga gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaatacga
    40681 ctcatatcgg tatctatatc tgatacaacg gatccccttc ccgcccagcc attagcgtat
    40741 tgccctggtc tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggtttt
    40801 tcctacgcac cagcatcaac tggcgttcca ccaccggcgt aatgcgcttt accaccagcg
    40861 ggctaccttc aggcaatggt agcgccagtg cgggaagaat actgatgccg atgcctgcgg
    40921 ctaccatcgg gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac
    40981 cattgcgcgc cagcgctgcg tcaatcagcg gtcggctgcc tgacgcataa tcctgcaaca
    41041 ccaatttcgc tccctgtaat gcctgccagg gtacataatc ttccgcggcc aaagcgctat
    41101 cccgatggca aagcagaaag aaaggctcgg aaagaatcac ttcacattgc agatcgccca
    41161 ctgggcctgg atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca
    41221 cccactgctg cggacgatcg tgcaggacaa actgaatatc tggatagcgg cgatggcttt
    41281 cagcaatgca ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt
    41341 ttccgctcag ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca
    41401 gcagtcgctc aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg
    41461 tggttctgtc cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta
    41521 ctgccgactg gctcaggccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta
    41581 ctgtgacgaa aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga
    41641 tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct
    41701 ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg
    41761 gtgttgctgg cctctttctt tcctgccaga ggcgatttcg tccccttctt tgaaaatctg
    41821 accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg
    41881 attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg
    41941 ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg
    42001 ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc
    42061 ttcacgtcaa tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagt
    42121 ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca
    42181 gggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg
    42241 gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg
    42301 aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt
    42361 aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc
    42421 gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact gggcttcaat
    42481 aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc
    42541 ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg
    42601 attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag
    42661 accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc
    42721 aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc
    42781 tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc
    42841 ggcaggtcga ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt
    42901 tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc
    42961 gatgacttca atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc
    43021 tatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag
    43081 tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt
    43141 accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac
    43201 accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg
    43261 aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc
    43321 ggcttccagc gcttccagcg ttccgaaata tgccgccaga cctgctgcgg tggcctcgcc
    43381 gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc
    43441 cagcgcgtta accacgtttt gtgccgattt tggccccata cgctccagtc cggtcagttt
    43501 gcctgcggtg agtttgaaca gatccgccgg agtgtggaca tattcttttt caaccagctg
    43561 atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt
    43621 cagcgactct ttacgctgcg caccgcaaat cagaccacca gtacagcggg caaccgcttc
    43681 accttccaca cgctcaacgt cagaaccaca taccggacaa tacgtcggga atacaacctc
    43741 acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc
    43801 agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc
    43861 attgtgtaag gttgcgttac tcaccagcac gcctgcaaca tggacaggtt ccagacgcgc
    43921 aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat
    43981 ctgttcctgc gccgggaatt taaacgctac cgcccagcgc ggggctcgcg cgacaaagcc
    44041 aagctgctcc tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc
    44101 cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc
    44161 acaaagcgtt acccgatcgc tgaccggcaa cccccacttt ttaaattgca gtaaacggcc
    44221 aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt
    44281 gagcggtcgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt
    44341 acgtgggtta gcaaacactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa
    44401 ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc
    44461 gtgcaacttc agtggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt
    44521 ggtgccatcg ccacgagtcg cggcactgac taaaacgcca ttttcataca gaatactgac
    44581 ggcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgc ttttcagacg
    44641 gtcctgcaca cgtttgttga aggcaagaaa gctttcttca tcaaaaacgt tatccagcga
    44701 cagcattggc acttcatggc gaatctggct gaaagccgcc agcggcgcag cacctacacg
    44761 ttgggtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc
    44821 gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag
    44881 atattcatga tggcgaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat
    44941 atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc
    45001 cttaagtgta tcaggcgttg gcgtctttaa cttcgcggat gatgtcctgg tactcgcgca
    45061 atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg
    45121 caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg
    45181 gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg
    45241 ttcccggttt caccatattc gccaggctaa ataacgccgg gccgctgcca tccgggctaa
    45301 gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa
    45361 gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg
    45421 cttctttgcg cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg
    45481 gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca
    45541 ctggctgcgg cgctggcgcg acctgtggcg aaactggctg ctgcaacggc tgttcaggct
    45601 gcggctgata ggcaggctgc tgcacgggtt gctgcaccgg ctgcgctgga cgcggagcat
    45661 gttgcggcgg tacctgcgct tcaggcggct gctggaccgg ttgacgcggc tgcgcagacg
    45721 cataaggcgg ttggtactgg tgttgcggcg acggacgagc agcctcatgc tcctgggcgt
    45781 tagccggggc atgattcacg cggtgaacac gaacctcacc aacgccctca tcatcttcga
    45841 catcctcgtc ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat ggccgatcgc
    45901 ggaacataga agatcgttct ttacggctgg tccagaaacc atgtaccagt aaagcgatta
    45961 tggcgatcgc gccaacaatg attaatatca gacgcaaatc ctgcatcatt atattctctg
    46021 ttgttctaac accttgccac cacggcaaac atttactcac taagagtatt tgccgattac
    46081 ctcaagtgca agtgcactat taactttcac agcacaaaga tagatgaaat cgtgcttttt
    46141 gctgtttttt cgaacatatc ctaactgtcc attgcgcaat tacccggtaa aatacgcaca
    46201 attttcctgg gattggtcaa aaggagctca tcctgattat ggtttcatca ttcacatctg
    46261 ccccacgcag cggtttttac tattttgcgc aaggctggaa gctcgtctcg caacctggga
    46321 ttcggcgttt cgttatttta ccgctgctgg tcaatatttt gttgatgggg ggcgcattct
    46381 ggtggctctt tacgcaactc gatgtctgga tcccgactct catgagttat gttccggact
    46441 ggttacaatg gctgagctat ctgttgtggc ctctggctgt catctctgtg ctgttagtgt
    46501 ttggctattt cttctccacg attgctaact ggattgccgc tccgtttaac ggtttattgg
    46561 ctgaacaact ggaagcacga ttgactggcg ctacaccgcc agataccggg attttcggta
    46621 tcatgaaaga tgtgccgcga atcatgaaac gcgaatggca aaaatttgcc tggtatctgc
    46681 cacgcgcaat tgtattgcta attctttact tcatccctgg tattgggcaa accgtcgcgc
    46741 cggtactgtg gttcctgttt agcgcctgga tgttagccat ccagtattgc gattacccct
    46801 tcgataacca caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca cgcaaaatca
    46861 ccaatatgca gtttggtgct ttaaccagtc tgtttacgat gatcccgctg cttaatctgt
    46921 tcattatgcc cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc tatcgcgata
    46981 aacacgcgat gtggcggtaa caatctaccg gttattttgt aaaccgtttg tgtgaaacag
    47041 ggggggctta tgccgcccct tattccatct tgcatgtcat tatttccctt ctgcatatag
    47101 atatgctaaa tccttacttc cgcatattct ctgagcgggt atgctacctg ttgtatccca
    47161 atttcataca gttaaggaca ggccatgagt aagatttttg aagataactc gctgactatc
    47221 ggtcacacgc cgctggttcg cctgaatcgc atcggtaacg gacgcattct ggcgaaggtg
    47281 gaatctcgta accccagctt cagcgttaag tgccgtatcg gtgccaacat gatttgggat
    47341 gccgaaaagc gcggcgtgct gaaaccaggc gttgaactgg ttgaaccaac cagcggtaat
    47401 accgggattg cactggccta tgtagctgcc gctcgcggtt acaaactcac cctgaccatg
    47461 ccagaaacca tgagtattga acgccgcaag ctgctgaaag cgttaggtgc aaacctggtg
    47521 ctgaccgaag gtgctaaagg catgaaaggc gcaatccaaa aagcagaaga aattgtcgcc
    47581 agcaatccag agaaatacct gctgctgcaa caattcagca atccggcaaa ccctgaaatt
    47641 cacgaaaaga ccaccggtcc ggagatctgg gaagataccg acggtcaggt tgatgtattt
    47701 attgctggcg ttgggactgg cggtacgctg actggcgtca gccgctacat taaaggcacc
    47761 aaaggtaaga ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc agttatcgcc
    47821 caggcgctgg caggtgaaga gattaaacct ggcccgcata aaattcaggg tattggcgct
    47881 ggttttatcc cggctaacct cgatctcaag ctggtcgata aagtcattgg catcaccaat
    47941 gaagaagcga tttctaccgc gcgtcgtctg atggaagaag aaggtattct tgcaggtatc
    48001 tcttctggag cagctgttgc tgcggcgttg aaactacaag aagatgaaag ctttaccaac
    48061 aagaatattg tggttattct accgtcatcg ggtgagcgtt atttaagcac cgcattgttt
    48121 gccgatctct tcactgagaa agaattgcaa cagtaatgcc agcttgttaa aaatgcgtaa
    48181 aaaagcacct tttcaggtgc ttttttgtgg cctgcttcaa actttcgccc ctcctggcat
    48241 tgattcagcc tgtcggaact ggtatttaac cagactaatt attttgatgc gcgaaattaa
    48301 tcgttacagg aaaagccaaa gctgaatcga ttttatgatt tggttcaatt cttcctttag
    48361 cggcataatg tttaatgacg tacgaaacgt cagcggtcaa cacccgccag caatggactg
    48421 tattgcgctc ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac aggctaaagt
    48481 cgaaccgcca ggctagactt tagttccaca acactaaacc tataagttgg ggaaatacaa
    48541 tgttccagca agaagttacc attaccgctc cgaacggtct gcacacccgc cctgctgccc
    48601 agtttgtaaa agaagctaag ggcttcactt ctgaaattac tgtgacttcc aacggcaaaa
    48661 gcgccagcgc gaaaagcctg tttaaactgc agactctggg cctgactcaa ggtacagttg
    48721 tgactatctc cgcagaaggc gaagacgagc agaaagcggt tgaacatctg gttaaactga
    48781 tggcggaact cgagtaattt cccgggttct tttaaaaatc agtcacaagt aaggtagggt
    48841 tatgatttca ggcattttag catccccggg tatcgctttc ggtaaagctc tgcttctgaa
    48901 agaagacgaa attgtcattg accggaaaaa aatttctgcc gaccaggttg atcaggaagt
    48961 tgaacgtttt ctgagcggtc gtgccaaggc atcagcccag ctggaaacga tcaaaacgaa
    49021 agctggtgaa acgttcggtg aagaaaaaga agccatcttt gaagggcata ttatgctgct
    49081 cgaagatgag gagctggagc aggaaatcat agccctgatt aaagataaac acatgacagc
    49141 tgacgcagct gctcatgaag ttatcgaagg tcaggcttct gccctggaag agctggatga
    49201 tgaatacctg aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc tgctgcgcaa
    49261 catcctgggc ctgaagatta tcgacctgag cgccattcag gatgaagtca ttctggttgc
    49321 cgctgacctg acaccgtccg aaaccgcaca gctgaacctg aagaaggtgc tgggtttcat
    49381 caccgacgcg ggtggccgta cttcccacac ctctatcatg gcgcgttctc tggaactgcc
    49441 tgctatcgtg ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact atctgattct
    49501 ggatgccgta aataatcagg tttacgtcaa tccaaccaac gaagttattg ataaaatgcg
    49561 cgctgttcag gagcaagtgg cttctgaaaa agcagagctt gctaaactga aagatctgcc
    49621 agctattacg ctggacggtc accaagtaga agtatgcgct aacattggta cggttcgtga
    49681 cgttgaaggt gcagagcgta acggcgctga aggcgttggt ctgtatcgta ctgagttcct
    49741 gttcatggac cgcgacgcgc tgcccactga agaagaacag tttgctgctt acaaagcagt
    49801 ggctgaagcg tgtggctcgc aggcggttat cgttcgtacc atggacatcg gcggcgacaa
    49861 agagctgcca tacatgaact tcccgaaaga agagaacccg ttcctcggct ggcgcgctat
    49921 ccgtatcgca atggatcgta aagagatcct gcgcgatcag ctccgcgcta tcctgcgtgc
    49981 ctcggctttc ggtaaattgc gcattatgtt cccgatgatc atctctgttg aagaagtgcg
    50041 tgcactgcgc aaagagatcg aaatctacaa acaggaactg cgcgacgaag gtaaagcgtt
    50101 tgacgagtca attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa caattgcacg
    50161 tcatttagcc aaagaagttg atttctttag tatcggcacc aatgatttaa cgcagtacac
    50221 tctggcagtt gaccgtggta atgatatgat ttcacacctt taccagccaa tgtcaccgtc
    50281 cgtgctgaac ttgatcaagc aagttattga tgcttctcat gctgaaggca aatggactgg
    50341 catgtgtggt gagcttgctg gcgatgaacg tgctacactt ctgttgctgg ggatgggtct
    50401 ggacgaattc tctatgagcg ccatttctat cccgcgcatt aagaagatta tccgtaacac
    50461 gaacttcgaa gatgcgaagg tgttagcaga gcaggctctt gctcaaccga caacggacga
    50521 gttaatgacg ctggttaaca agttcattga agaaaaaaca atctgctaat ccacgagatg
    50581 cggcccaatt tactgcttag gagaagatca tgggtttgtt cgataaactg aaatctctgg
    50641 tttccgacga caagaaggat accggaacta ttgagatcat tgctccgctc tctggcgaga
    50701 tcgtcaatat cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt ggtgatggta
    50761 ttgctatcaa accaacgggt aacaaaatgg tcgcgcctgt agacggcacc attggtaaaa
    50821 tctttgaaac caaccacgca ttctctatcg aatctgatag cggcgttgaa ctgttcgtcc
    50881 acttcggtat cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt gctgaagaag
    50941 gtcagcgcgt gaaagttggc gatactgtca ttgaatttga tctgccgctg ctggaagaga
    51001 aagccaagtc taccctgact ccggttgtta tctccaacat ggacgaaatc aaagaactga
    51061 tcaaactgtc cggtagcgtg actgtgggtg aaaccccggt tatccgcatc aagaagtaat
    51121 tcttgctgca gtgaaaaatg gcgcccatcg gcgccatttt tttatgcttc cgccagcggc
    51181 ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc
    51241 aaccccgcgc ggtgcaccgc atcgtttaat gccttccctt tcagcaaacc actgataagc
    51301 tgagcacaaa acaggtcgcc agtccctttc aggtcggttt ttacccttga atgggaaatg
    51361 acattcacgc tgtcggcagt gaccaccaca accagcatct cctgattttc ttcattaccg
    51421 gaggcgctgg taatcaccac ccattttagt gtgtctgaaa gcagactttt tgcggcagca
    51481 atggcactgt cgagatcgcg gcaattttta ccggtcagga tttccaactc aaagatattg
    51541 ggggtaattc cctgcgccag cggcagtaaa tattgtcgat acgcttcggg aaggtcaggt
    51601 ttgacataaa ttccgctgtc aatatcgcca atcaccggat cgaccatgat caataggtca
    51661 ggatggtctt tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt
    51721 cccatatagc ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagtgca
    51781 cgcaaatagc cgctaaacca ttcatccgga atcgcaccac cgtagaaagt gtcataatgc
    51841 ggcgtattgc tcagcaatac cgtcggcacg gcaaagacat tcaggccgtt ctgtttgata
    51901 gcaggcacgg caatgctgtt gcccacgctg ccgtaaacca cctgcgactg cacggcgacg
    51961 atatccgcct gcagcgccct gctcttatcg ttaaacaaca acaaactact catttaattt
    52021 tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt
    52081 caaatcggct attacaaaac aaggataagg taattcagtg aagaaaatca tttgtctggt
    52141 cattacacta ctaatgacac tccccgctta cgcgaagtta actgcccatg aagaagcccg
    52201 catcaacgcc atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg
    52261 tgatgaacat acctgcgatg aagcggtttc tcatctgcgt ctgaagctgg gcaatacccg
    52321 taaccgcatt gacactgccg agcagtttat tgataaggtt gcttcgtcgt catcgattac
    52381 cgggaagccg tatattgtga agatccccgg taagagcgat gagaatgcac agccttttta
    52441 catgcgttaa ttgcgcagac ggataaaacg gtgcctgcgc aataatctta aatccccgcc
    52501 ccctgactaa aatgctcttc cccaaacacc ccggtggaaa ggtagcgatc gccacgatcg
    52561 cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag tgctccggca
    52621 accgcgccgc cggagctgac gccacagaat attccttccc gcaccgccag ttcgcgcatg
    52681 gtgttttccg catcgcgctg atgaatatcc agcacctcat ctaccagaga agcgttgaaa
    52741 atccccggca gatattccgc aggccagcgg cgaatgccgg gaatgctact gccctcttcc
    52801 ggctgcaggc caacaatggt caccggtttg gattgttcgc gcataaagcg tgaaacgccg
    52861 gtgatagtgc cggttgttcc catgctggag acaaaatagg taatgcgccc gccagtctgc
    52921 tgccagattt ccggcccggt agtggtgtaa tgggcgtaag ggttatcggg attattgaac
    52981 tgatcgagca gctttccttc accacgatcc gccatctcca gcgccagatc gcgcgcacct
    53041 tccatgccct gctctttggt gaccagaatc agttccgcac cataagcacg catcgccgca
    53101 cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc tttaagcgcg
    53161 gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa cacatcaccc
    53221 ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagcgccgc acgatctttt
    53281 accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc gttattcggc
    53341 cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa tgtactcacg
    53401 atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc aggcctacag
    53461 gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg cgtttaacat
    53521 tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatctcggg gttcgatacg
    53581 ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct cgccacgctg
    53641 cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg
    53701 caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta ccggcagcgg
    53761 cgaatcgagg ctggtacggc ggctgatatc cacttcccac gggcgcagga agagatccac
    53821 cggcccctga tacgcaggtg tgtagcccag cggccagcga tgtgcgccaa catggaactg
    53881 cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga gcacaaaacg
    53941 ggtcgccggt tcgcgccata cctgatccgg cgcgtcagcc tgttcaatat tgccctggct
    54001 catcacaact acacgatcag ctacttcggt cgcttcttcc tgatcgtggg tcacaaaaac
    54061 gctggtgaat tttagttctt catggagttg acgcagccat cgacgcagct ctttacgcac
    54121 ctgcgcatcc agcgcgccaa acggttcatc aagcagcaga atttgcggtt ctacagcaag
    54181 cgcacgcgcc agcgctacac gctgtttctg gccgccggaa agctgcgccg gataacgatc
    54241 cgccagatgg gcaagctgga ccatttccag caattttgtc actttcgctt tgatggctgc
    54301 ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca aaagcgatat tgtcgaacac
    54361 cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa ccgactttac gatcacgtgc
    54421 gtgcaggcgg ctcacgtcgg tgccgtggaa gcgaatatgc ccgctggttt gatgctccag
    54481 cccagcgata atgcgcagca gcgtggtttt cccggaaccg gacggcccca gcaacgcgac
    54541 catctgacct gaaggaatat ccagtgagat atcgttcagc acctgggtgc gaccaaacga
    54601 cttcttaata ttggcaatct caatgctcat gattttcctc ctgctgcgcg cgtttttcct
    54661 gattctccag gcgccactgc aacatactct ttaaaaacag ggtgataatc gccatcagcg
    54721 tcaacagcgc ggcggcggta aaggagccga ctgtgttgta gtcctgctcc agcaattcaa
    54781 tctgtaacgg cagcgaaagt gtttcgccac gaatcgagcc ggaaaccacc gacaccgcgc
    54841 caaactcgcc aatcgcgcgg gcgttggtca gcactacgcc ataaagcagc gcccagcgga
    54901 tgttcggcaa tgtgacacgg cggaacatct gccagccgga tgcgccaagc aaaatcgccg
    54961 cttcgtcttc ctgactgccc tggcttaaca tcaccggcac cagttcgcgc accacaaacg
    55021 gacacgtcac gaagatcgtg accagcacca ttccgggcca ggagaacata atttgcagat
    55081 tatgttcatc cagccagccg ccgagcgggc cgttggagcc gtagaacagc agatacacca
    55141 gaccggcaac caccggtgac acggcaaaag gaatgtccag tagcgtcagc agtaactggc
    55201 gtccggggaa gttaaagcgc gtcaccagcc aggccagcag aataccgaac accaggttta
    55261 ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc atatccggat
    55321 cggccagatt ctgtaaaacc ggcatcagcc ctttgctgaa cgcctgtacg aagatataaa
    55381 tcatcggcac cagcaggatg aacgccgata ccagcatccc gatgccaatc agaaaccatt
    55441 tgccccagtt aatcgggcgc gcgtcataac gcttcaattg tgtcacttcc gccattaatg
    55501 acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca gcagcagcag
    55561 agatgccgcg aggatcaccg aagcaatcgc gctcgccgcc gggtaatcaa actcctgtaa
    55621 gcgaacgaaa atcatcagcg acgtcacttc cgtcttccaa gcgatgttcc cggcgataaa
    55681 tatcaccgcg ccaaactcac caagactacg ggtaaacgac aacgccacgc ccgccaccag
    55741 cgccggagaa agttccggca gcaccacttt gcagaaactc tgccagcgcg ttgcgccaag
    55801 cgtttctgcc gcttcttcat attccgggcc taactcttcc agcaccggct gcacggtacg
    55861 taccacaaac ggaatgctgg taaaggccat tgccaccgca atccccagcc aggtataggt
    55921 gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacag aaaagagcga
    55981 ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc
    56041 aagcagcgtg cggcccggaa agcgataacg ggttaaaatc cacgccatca gcaggccgaa
    56101 aacgccgtta aaaattgatg ccacaaacgc cgacagcagc gtcactttgt aggccgcgac
    56161 cacctgcggg ttggtgatca cttcccagta ctgcgcccag ctcatctcag aaagctgcat
    56221 caccagcgcg gagagtggca gcagcaatat caggcacaca aacagcagac tggtgccgag
    56281 gcttaaggta aagcccggca ggacgcgtct tgatgaaact gcaaacatta attacgcccc
    56341 gccgctaaca gcttgtctaa ctcgccgccg ctggtaaagt gggttttcat cacttccggc
    56401 caggagccaa atttgtcttc cacgcggaac agttcggtct gcgggaattt atctttcagt
    56461 ttgtccatga cttccgggtt attcacgcgg tagtaatagt cggtgatgat ggtttgcgcc
    56521 tgcgggctgt aaagccagtt cagataggct tttgctgctt tttcggtacc gttggcttgc
    56581 acgtttttat cgacccacgc caccgggaat tccgccagaa tgttggtttt cggaatcacc
    56641 acttcaaagc cctgcgcttc gtactgtttg cggatgttgt tcacttccga ttcaaagcta
    56701 atcagtacat cgcccaggcc gcgctcggca aaggtggtgg tcgcgccacg accgccggta
    56761 tcgaacactt caacgttttt caggaactga gtcataaact gttcggtttt ggctttgtcg
    56821 ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg cgcgttaccc
    56881 gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc gttccagtcg
    56941 tggatattct tcgggttgcc cttacggacc aggaagccca tggtggagta gaacggcgag
    57001 ctgttattcg gcaggcgcga ctgccagtcc gccgggataa gcttgccttt atcgtgcaga
    57061 atttgtacgt cggtcacctg gttataagtg acaacgtcgg cttttaagcc ctgcaaaatt
    57121 gccagcgcct gttttgatga cccggcatga gattgtttta tcgtcagttt gtcgccgccg
    57181 ttatctttgg cccattgctg ttcaaacggc gggttcaggg cggcaaacag ctcgcgggag
    57241 acgtcataag aactgttcag cagttccgtt gcctgtacat ggcccgccag cagcagagaa
    57301 gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca cccttataaa
    57361 tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt aacggtttta
    57421 tataccgttt ggtgatttgg aagttgaaaa gggaataaga cgaaatcctc cccacaaatg
    57481 cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca gtgtgctgcc
    57541 gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg cgaggaaggc
    57601 cgccagttcg ccgacttcca gcggatcggc gaggcgacgc agcgggattg cttttgccat
    57661 ttcagtcagc accgactctg gatcttccgg gttcgactgg cgggcaatgc tttccgccat
    57721 tggtgtgcga acgtatccag ggcatatggc gttaacgcga ataccagact gcgcatactc
    57781 caccgccagc gattttgtca ggccaacaat cgccgctttc gttaaggcgt acgccgtttc
    57841 acccggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc gaccatcttt
    57901 gcgggcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt taatattgat
    57961 gtcaatatgg aaatcgcgat cgtcatcgct catatcgagg aaactgccca gacgacaaac
    58021 gcctgcgtta ttcaccagga tatcaatgcg cccttctttt tccttcgcgc gtttgatagc
    58081 tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc gatgaccacg
    58141 accacacagt tcgtccgcca gcttttcgat ctcaggggag atatccagca agattaggtt
    58201 tgcgccatga cgcgcaaaag ttctggcaat tccttcgcca attccctgca atgcgcccgt
    58261 aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt taatacagag
    58321 cgttacactc cattccctct gaaaatacag gataaacaaa atattactgc ggatcagctc
    58381 actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag gcgttgtgtt
    58441 agctcactgc tgcgctgaat cattttcaat gactccacat cattgagttg cacaagacca
    58501 acaaacagta aatcggtaac agaattttgc gccgtgcggg tagacatcga cgagctacgc
    58561 cattcggttt caccggaaac ggtatcgagc gtgaaatgcg ccagccgccg cagcggagag
    58621 tcagccagag aagtaatggc aatcaccgtt gccccctgct ttcgtgccgc ttctgcgcac
    58681 aagacaatct cttttttact gccgctgtaa gagatagcga tttgtacatc gccttttttc
    58741 aacgcctggg aaacggtggc ttgtacgtgc gtgtcggctt cgcaggccac gcgatatcca
    58801 attttcatca gtttgaacga cagatcgcac cccaccagcg ccgatccgcc caggcccgtt
    58861 atctggataa acggtgcttt actgatgacc tcaatgattt tttgcagccg cgcgtagtcg
    58921 aacaacgcac aggtttgctc cagcgccagc tctttttcgc gattcaattt gcgggcaatc
    58981 acttccagcg aatcatcact ggtaatcgaa ctgtgcaggt gctgcgccgt ggcattcgtt
    59041 ttctcccggc tggcactgta ttcgcctatt aacgccatac gcaattcagt aaacccttgt
    59101 gcgccgagct tctgggcaaa cttcacaata ctcgactggc tgatgccgag ctgtttcgcc
    59161 atcttgcgcg aagagacgga tttcagctca ctcacgttgg cccgcagaaa gtcagcaatt
    59221 ttttgctcgt tctcggtaaa ttcactttcc gcattgcgta ttttcgtcag gtacaacatc
    59281 ttccactctc ctgggctggc agcaaaaatt ccgcttaagt tcttatgacg ctcttcacac
    59341 tctgcgagtg tagactcaat gattccttta agtctgtcaa ttcgggatat atgattccat
    59401 acatctgcaa ttatttgatc gtaaatagta aggtcaccac cgatgcaact tgaaaagatg
    59461 attaccgaag gctcgaacgc cgcctcggct gaaattgacc gcgtatcaac gctggaaatg
    59521 tgccggatta tcaacgatga agataaaacc gtaccgcttg ccgttgagcg cgtactgccg
    59581 gatatcgccg cggcaatcga tgttatccac gcccaggtaa gcggcggcgg tcgtctgatt
    59641 tatctcggtg cgggaacatc cggtcgtctg gggattctgg atgccagcga atgtccgccc
    59701 acctacggcg tgaaaccggg tctggtagtt ggattaattg ctggcggcga atatgccatt
    59761 cagcacgcgg tggaaggcgc tgaagatagc cgggaaggcg gtgttaatga tttgaaaaac
    59821 atcggattaa ccgcgcagga tgtggtggtt ggcattgctg ccagcggtcg cacgccgtat
    59881 gtgattgccg gactggaata tgcacgccag ctcggctgcc gtacagtggg aatttcctgt
    59941 aatccgggga gcgccgtttc caccaccgct gagtttgcta ttacgccggt ggttggggct
    60001 gaagtggtca ccggttcttc gcggatgaaa gcaggcacgg cgcagaaact ggtgctcaat
    60061 atgctttcca ccgggctgat gattaaatcc ggcaaagtgt tcggcaacct gatggtcgat
    60121 gtggtcgcca ccaacgaaaa actgcatgtg cgccaggtca atatcgttaa aaacgccacc
    60181 ggatgtaatg ccgaacaagc agaagcagcg ttaattgcct gcgagcgcaa ctgtaaaacg
    60241 gccattgtga tggtgctgaa aaatctcgat gccgcagaag ctaaaaaacg cctggatcaa
    60301 cacggcggct ttattcgtca ggttttagac aaggaataac ccatggccaa agagatcagc
    60361 agtgaacttc tgaacaccat tcttacccgt gtcggcggac cgggaaatat cgccagttgt
    60421 ggtaactgta tgacgcgcct gcgtctgggt gtacatgaca gttcactggt tgatcccgat
    60481 atcaaaacgc tggaaggcgt gaagggcgtc attttgacca gtgaccaggt gcaggtcgtt
    60541 tttgggcctg gtaaagcgca tcgtgccgca aaagcgatga gcgagctgct gggagaagca
    60601 ccggtacagg atgccgcaga aatcgccgcc cagaacaaac gtcagttaaa agccaaacaa
    60661 acctccggcg tgcaacaatt tctcgccaaa ttcgccacca tcttcacgcc gcttatcccc
    60721 ggttttattg ccgccggtct gctgctgggg atagcgacgt taattgccac ggtgatgcac
    60781 gttccggcag atgctcaggg cacgctaccc gacgcgctga attttatgaa ggtgttcagc
    60841 aaaggtttgt tcaccttcct ggtgattctg gtgggctata acgccgccca ggcatttggc
    60901 ggcacgggcg tcaatggcgc aattattgcc gcactctttt tgctcggtta taaccccgct
    60961 gcaaccaccg gttactacgc cggttttcac gatttctttg gtctgcccat cgatccgcgc
    61021 ggcaatatta tcggcgtgtt gattgccgcg tgggcctgcg cccgcatcga aggcatggtg
    61081 cgccgcttta tgcccgacga tctcgacatg ctgctgacct cgttaatcac cctactgatc
    61141 accgctacgc tggcctatct gatcattatg ccgctgggcg gctggttatt cgaaggtatg
    61201 tcgtggctgt ttatgcacct gaacagtaat ccgctcggtt gtgcggtttt agccgggctg
    61261 ttcctgatcg ccgtggtatt tggcgtgcat cagggcttta ttcctgttta cctcgcgtta
    61321 atggacagcc aaggattcaa cagcttattt cccatccttt cgatggcagg cgcgggccag
    61381 gtgggcgcgg cgctggcact ctactggcgg gcgcaaccgc acagtgcatt acgcagtcag
    61441 gtacgcgggg cgattattcc cggcctgctg ggcgttggcg aaccgttgat ttatggcgtc
    61501 accctgcccc gcatgaaacc gtttattacc gcctgtttag gcggcgcggc gggcggtttg
    61561 tttatcggat tgatagcctg gtggggtctg ccgatgggct taaacagcgc ctttgggccg
    61621 tctggtctgg tggcgctgcc gctgatgact tcagcacaag gcattcttcc ggcgatggca
    61681 gtctacgctg gcgggattct ggtggcatgg gtttgcgggt ttattttcac cacgcttttt
    61741 ggctgccgta acgtcaatct ggactgatat catgaaacgg acaatgctct atctttctct
    61801 gctggctgtt agttgtagcg tcagcgccgc aaaataccct gttctgacag aaagttcgcc
    61861 agagaaagca gggttcaacg tcgaacggct taaccagatg gatcgctgga ttagccagca
    61921 agtggacgcc ggttatcccg gcgtaaacct gctgattata aaagataatc agattgtgta
    61981 tcgcaaagcc tggggagcgg cgaaaaagta cgatggcagc gtattgatgg cgcagcctgt
    62041 aaaagctacc accgggacgc tgtatgacct ggcctcaaac accaaaatgt atgccactaa
    62101 cttcgctctg caaaagctaa tgagcgaagg caaactgcat cctgacgatc tgattgcgaa
    62161 atatatttcg gggtttgccg atagtccaaa tgacaccatc aaaggcaaaa acacgctgcg
    62221 gatttctgac ctgctgcatc acagcggcgg tttcccggcg gacccacaat atccgaataa
    62281 agcggtcgcg ggcgcgttat attcccagga taaaggccag acgctggaga tgatcaagcg
    62341 cacgccgcta gagtatcagc ccggcagcaa gcatatttac agtgatgtcg attatatgct
    62401 gcttggattt atcgtcgagt cggtcaccgg tcagccgctt gaccgctatg ttgaagagtc
    62461 gatttatcgc ccgctcggcc tgacacatac ggtatttaac ccgctgctga aaggctttaa
    62521 accgcaacaa attgccgcca cggaattgaa cggcaatacc cgcgatggag tgatccactt
    62581 cccgaatatc cgcacctcca ctcactgggg tcaggtgcac gatgaaaaag ccttttattc
    62641 gatgggcggc gtttccgggc acgcaggttt gttttccaat accggcgata ttgcggtgtt
    62701 aatgcaaacg atgctgaacg gcgggggcta tggcgatgtg cagctgttca gtgcggaaac
    62761 ggtgaagatg ttcaccacca gctctaagga agatgccact tttggcctcg gctggcgcgt
    62821 gaatggtaat gccaccatga cgccgacgtt tggcacgctg gcaagcccgc aaacctacgg
    62881 tcacactggc tggaccggaa cggtgaccgt tatcgatccg gtgaatcata tggcgattgt
    62941 gatgttaagc aacaagccac attcgccggt tgccgatccg caaaagaatc ccaatatgtt
    63001 cgaaagcggt cagctgccga ttgcaacgta tggttgggta gttgatcagg tgtatgcggc
    63061 gttaaagcaa aagtaataaa aaggccagtc agtaatgtta ctgcctggcc tttaatcaat
    63121 gaatcagaaa cgcttacagc gccatcaact tgtccagcga cggtgcgaaa taatagccgc
    63181 cggttaccgg tttggtgaaa cgcaacatcg catcacgctt accatcggta tcgccaaaca
    63241 tgctcagcag ttgctgctca atgttatgca gacgcgcgca gtaggcgcag aagtacagac
    63301 cgtgagtgcc actggcggtg ccgtacggca ggctctggcg aacaatcttc agccctttgc
    63361 catcttcttt cagatcaacg cgggtcaggt gagaggtttc cggacgttcg tcgccgtcaa
    63421 tctcttcgtt ggcctctttg gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc
    63481 ggttgagctg cttcaggttg tgctcccaac gctgcacaaa cacatagctg ccgccggcat
    63541 ccacgccgtc tttgataacc gccacttcgc gacgcgtctc ttcacccgcc gggttttctg
    63601 tgccgtcaac aaagccgctc aggtcacgct cttccaccca acggaagccg tggatctctt
    63661 ctttcacttc aatgcagtca ccaaaggctt ccatcgccgc ctgggcgaca gagaagttta
    63721 cgtcgtgacg cagagaaaga atgtggatca acacatcgaa ctgggtggtc ggcgcaaggc
    63781 ctttaccgta gcccggaaaa tctttcagct cttctgcccc aacgccgccg ctcagagcgc
    63841 gccagatgtt gttaccaaag gcaaccaccg caccaagatg cgcgtccggg aatttcgctt
    63901 caaaagttgc cagtttgtcg gcaaatgttt tactggccgc acgcagggcg tcaacttccc
    63961 ctttcacgtt ggcttcgatc caaatcgccg cgcggcaatg ttctggcaaa atgccactct
    64021 gaacctgaga cattgttcct cctgaaaata ataatgccac gcaatccgtg gcattgttga
    64081 gcgctatttt acgtgttttt tcgcggcggc ggtttgttca gacgcaaatt aacgccgcca
    64141 gataatttta ctgactttcc agtttttcag ggtatcgtcc gaaggcatta acccttcagg
    64201 accactccat tccccgctga actggtagct aatatgttga ctgccttcgg ctttacattc
    64261 gacggcacga ttatcatcac catcagcttt ttggcaattg ccaaatgctt tgctgtaaag
    64321 gtcgctaaac ggtgtaccga ttttaacacc ggtgtcagca ggaatatcgc tatccagcac
    64381 gtcaatgcga ctgatcgtgc cttgatcgcc gttaatcacc atcgccacgt tgtcgccctt
    64441 catcacttca aaaaagcgca ccacgttgcc gttcgcggtt ttcattccgc tgcgcaggcg
    64501 ataatcgcca tcaaccgcat cggcaatggc ttgttcttgc agtggtgtgg acgccgttaa
    64561 ttcaccaacg ccctgctcgc tcactttggt ggacgaccca aaccagttcc acgggttagc
    64621 ggcagaccag ttaactgacg acatcgtgga acagccggtc agcatcaatg gcatagcgca
    64681 taacattaaa cgcagcgatt tcatgtcact tcctttggtt attcaataac gttgcttgga
    64741 gtgcaaattc accaaaaagt gccgttattc ttttacttca ttaaaacaag cgcgtaaacg
    64801 tcgattggtc agcagccaga ttaacgccac aatatccgcc actaccagcg ccagaccgat
    64861 accgctaacg gattcaccgt tcagccacag ccacggttgc cagcaaagta aaaccacctg
    64921 cgccaacagc aacagaaaat agagtacacg ccaggtgcga ggaaacgtag cccgccgacc
    64981 gctcaacaaa aacgccagca ccgccggaat gccaggaatc agccccagcc agaaattatc
    65041 gtgatcggga taaaacagat ttagcagcgc agtaccctgc tcgcgcgacg caccggcaat
    65101 gacaaacagc acccaggttc gcgcctgaag caatagcaca agccagaaga gcaagggtaa
    65161 acgcaggcga ccgtgcgcat cataatggac aggatgaaac tcagtactct tcatcttcaa
    65221 tcaaacgctt acccagactc agcacgtcgg cgtgttcata tcccaggcgt tcatacattc
    65281 cgagcaccat gtcgttatct tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa
    65341 tcagcttttt ctccagccgg ttaagcaacg cattggcaat cccacgccca cgaaactctg
    65401 gatgcacgcc aagataataa gcagacccgc gatgcccgtc ataaccgccc atcaccgttc
    65461 cgaccacttc accgttcacc tcagcgacca gaaacaaact gacgtcatgg ttcatcttac
    65521 gctcgatgtc catttccgga tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg
    65581 tgatgacctc ttcgaaatct tcctggcgaa atacgcgtat ctccatggta ttcgttacct
    65641 ttttgcgggt taaaaggctg attatggcgt gaacggtcga attagccaat atctgacgaa
    65701 aatcggttga aaaagtggca taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca
    65761 attctcaaca gcaaacggtc gtaacggatt acgcgatacg atataacatc tggaacttta
    65821 ttattacaac tcaggccgta tgagcacttt taaaccacta aaaacactca cttcgcgccg
    65881 ccaggtgctg aaagccggtt tggctgccct gacgttgtca ggaatgtcgc aagccatcgc
    65941 caaagacgaa cctttaaaaa ccagcaacgg acacagcaag ccgaaagcta aaaaatctgg
    66001 cggcaaacgt gtcgttgttc tcgatccagg ccacggcggg attgataccg gagcgatcgg
    66061 gcgcaacggt tcgaaagaaa aacatgtggt gctggcgatt gctaaaaacg tccgttccat
    66121 tttgcgtaat catgggattg atgcgcgttt aacgcgttct ggcgatacgt ttatcccact
    66181 ttacgatcgc gttgaaatcg cccataaaca tggcgcagat ctgtttatgt caattcatgc
    66241 cgatggcttt accaacccga aagctgccgg tgcttcggta tttgccctct ctaaccgtgg
    66301 ggcaagtagc gcaatggcga aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc
    66361 cggtaaaaag gcgactgaca aggatcatct attgcagcaa gtgctgtttg atctggtgca
    66421 aaccgatact attaaaaaca gcctgacgct cggctcgcat attctgaaaa agattaagcc
    66481 ggtgcataaa ctgcacagcc gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc
    66541 gtcggttcct tcggtgctgg tggaaacctc gtttatcacc aacccggaag aagagcgact
    66601 gttaggcacg gcggcgtttc gtcagaaaat cgccacagcg attgctgaag gcgtgatcag
    66661 ttatttccac tggttcgaca accagaaagc acattcgaaa aagcgataag ttatgaaacc
    66721 cgacgcacac caggttaaac agtttctgct caaccttcag gatacgattt gtcagcagct
    66781 gaccgccgtc gatggcgcag aatttgtcga agatagctgg cagcgcgaag gtggcggcgg
    66841 cgggcgtagt cgggtgttgc gtaatggtgg tgttttcgaa caggcaggcg tcaacttttc
    66901 tcacgtccac ggcgaggcga tgcctgcttc cgccaccgct catcgcccgg aacttgccgg
    66961 gcgcagtttc gaggcgatgg gcgtttctct ggtagtgcat ccgcataatc cgtatgttcc
    67021 caccagccac gcgaatgtgc gcttttttat tgccgaaaaa ccgggtgcag agccagtctg
    67081 gtggtttggc ggcggcttcg atttaacccc tttctatggt tttgaagaag acgccattca
    67141 ctggcaccgc accgcccgtg acctgtgcct gccatttggt aaagacgttt atccccgtta
    67201 caaaaagtgg tgcgacgatt acttctacct caaacatcgc aacgaacagc gcggtattgg
    67261 cgggctgttc tttgatgatc taaacacgcc agatttcgac cactgttttg cctttatgca
    67321 ggcggtaggc aaaggctaca ccgacgctta tttaccaatt gtagagcgac gtaaagcgat
    67381 ggcctacggc gagcgcgagc gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt
    67441 caatctggtc tgggatcgcg gcactctgtt tggcctgcaa actggcgggc gcaccgagtc
    67501 gatcctgatg tcaatgccgc cactggtacg ctgggagtat gattatcagc caaaagatgg
    67561 cagcccagaa gcggcgttaa gtgagtttat taaggtcagg gattgggtgt aactccctca
    67621 cccccattcc cgcatccgct gatgcaacgt caacgacggc ttctcggcaa acagctgctg
    67681 gtaatccgtg gcaaattgcc ccagatgcca gaatccccac tgcatggcgg cgtcttttac
    67741 cgttgtgctt tgcgaccacg ggcttatcag ttcgcggcgt acggcgttca ggcgaatgcg
    67801 tttcagccac gcgttcgggc caattcctaa aatagcgtga aacgcgtttt gtagcgtgcg
    67861 gcggctgaca tgcagttgat tacacaagtc cagcaccgtc accggttcgg acatgttttc
    67921 cagcacatat tcacgggcgc gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc
    67981 cgccgtcacc attggctgcg cttcttccag catcgccccc atcgccatta gcaaattatc
    68041 ccccagcact tttcgcactg caggctgatg gagattttcc gggttctcgc aaaacgtcgc
    68101 cagcgcctgt tggacaaagc cccacagcgc ggctttatgc tgctctttca cttccagcgc
    68161 cgactggcta cgcaacatat gtaatacccg atccggatta tgcaaaaagt tagcctgacg
    68221 ggtgatgaca tcttcagaaa gcactacgcc caggatcgtg taatcgtccg gcgtacttaa
    68281 ttcaaactcg gttccaccag ggcgcgtcgc tatttctgcg cttcccagac attgcgaacc
    68341 gataaatccc tgctcaccgc gcgtcgccgg aatgccaaac cagaacgagt tcggccagac
    68401 cagacacgac tgacgcagcg ccagaccggt gtattcacga aaaacctgaa tatcatcgag
    68461 tagaatttcc gtaaattcac catgaaactt gcccggatgc agctgatcgt aaatctgctg
    68521 ccaggcggta atcgttaaag catgttcata gacatccgtt gtctgtcgtt gatgaacatt
    68581 atccacttcg accttcggcg tgagcttcag gttttcgggt aagggttcat gataaagatg
    68641 gtgcaaattg gctgtacggg tctttttcat gatgttaatg ccgggtgttg taggacaccc
    68701 ggcacctccg acaggttaat ggggcttgag acgataacga ctactgcgtt tacgtaacgt
    68761 cccagcagaa aagagctgtt cgagcgcatt tctggctttt tccagcggcc agccaaagtg
    68821 ggcagccact tctcctgccg tcactccctg acgtactgat gttaacagcg ccaacaactc
    68881 gtcagcagat tccgcgacga taactggcgc gtcgggttcc tttacgggtt gcttcggctg
    68941 gcggttaaag ccagtaacca gccactgggt gtcatcgtct ggccgaccga tctccttacg
    69001 gctgatgaca cgcccggtac gcatcgcggc agcacaacct gcgtccagcg cagcacgaca
    69061 cgccgccaga tcgccttcca ccaccagcgt taagcgacct gggtcaagca cttcgtgact
    69121 gagcagacga acgttagctg ctttgagcat ggcatccgct gcatctatcg cggcgaccat
    69181 gccgtccact tccagcaatc ccagtgcatt gatcattggc aacctccgtt acgcacgctg
    69241 gattggatta cgcgcgattt ccagcactgc atcggtaaag gcgttacagg ccgctttaca
    69301 cgctgcctgg ctaccggtta aaaatgctgc cgagtagtta gtttcagacg gtggcgggac
    69361 ataggtcacc agctgtacgt cggcagattt caacgctgca tcaatgccgt aggtcgcttc
    69421 cagcggcggt gccaccagat acgccatcgg atcgccaagc gtgatcccgg cggttgatga
    69481 gagataagaa ccggtacgcg aaactacatg tgccaggaat gccgtatctt gcgcatcatt
    69541 agcccactgg aaagcagcgc cattttcaat atgcgcaacc atcgcatcca gaccggcacg
    69601 cacttccgcc gggtttggcc caccaagcat aatcagcacc tcaccggcag tcggtgacgg
    69661 gccgtgtgcc gcgcccgcat acagcgagcg accatatacc acttctacca tcgcttgttt
    69721 ggtcgcttcg tcagccgcaa tgtaggttac gtcgtcagaa tcggcagaaa tcagaccgag
    69781 gctacgaata tgtggcggca atttcagttc acgcgcaaaa tcggcgttaa cggaggcaat
    69841 cacccgcatg gcggttaccg acggtcgaat caaatctaaa gctggcatga tgcctcctta
    69901 tcgggtcatg ttgatgccgg atgctttctg ctccagcata cgtttcgcca aatccacaat
    69961 gacggctgcg gcttcaaccg gcggcgtgcc gccctggtga atgttagaga tacaggtgcg
    70021 atcggcctca acggtggtcg ccatacgcgg cgagtaaacg gcgtagcagg agaggctttc
    70081 tgactgaccc aaccccggac gttcgcccac cagcaggatc actactttcg cgccgaggat
    70141 ctcaccgatc tgatcttcaa tcttcacgcg accataacga acaaagaatg gcgtgccgac
    70201 tttcagcccg gcctgtttca ggcccgccat cagcggcggc aggatctctt cgtagttcac
    70261 agtgatggca tcagttgaca ggccatcaga aataacgacc tgtacgtccg gattagcaac
    70321 acactgtgct ttcagcgctt caacagcttc tgcacacagg cggcggccca tatccgggcg
    70381 agtcaggtag agatttttgt cgctaatttc ggagcgtact tccagcaagc cctgcgcttt
    70441 cacccactct tccggcactt ctttcagtac ggtatctttc gaacgagagt gatcggccag
    70501 gaaacgcagc agcgccagcg tacgcggacg cggaccggca cgaccggtac aaacgcgggc
    70561 cacggtgctg cgacgcagtt ctgttaacac atccgcacga tgtgggtttt caacaccaat
    70621 ccacactttt gcttccgcag aacctaaatc cagcgcgcag ctttccgagg tcaccggtgc
    70681 cgcacaggtg gtagtggcgc actttgcttc tgacggcgcg ggggccgttt gtcccattga
    70741 cgccatcacg ctgcgtacaa tttcttcaat ctgtttttga tccatgatgt gttatctccg
    70801 cgtcatcaga agaacagtga cggatcgccc gcccgtttgg tcaggcgacc gtttgccata
    70861 atgcccatgc tttccagcca gcgttcaaac tccggtgacg ggcgcaggtt gagtaactga
    70921 cgcacagtgg cggtatcgtg gaatgcggtg gtctggtagt tgagcatgat gtcatcaccc
    70981 agcggcatcc ccatgatgta gttgcagcct gcggtggcga gcaggatcat caggttttcg
    71041 ttgaggttct ggtcagcgtc agcgtggttg gtgtaacagc agtcacagcc catagagatg
    71101 ccgctcagct tgcccataaa gtgatcttct aagcccgcac ggataatctg gcggtcgttg
    71161 tagagatact ccggcccaat aaagccgacc acggtgttga cgataaacgg atcgtaatga
    71221 cgcgccagcc catagttacg tgcttccatc gtcacctggt ctgcgccgaa gttagcgcca
    71281 gcggatagcg ctgaaccttg tccggtttcg aagtagaggc agttttcccc ggcgatacgg
    71341 ttgaactctg cacccactgc gcgcgcttcg tcgagcatcg ccagttccac gccaaactct
    71401 ttcagccctt tttcgctgcc acagatactc tggaaaatca gtccgccagg cgcgccgcga
    71461 cgaatcgctt cgatctgggt ggtgacgtgc gccagtacgc agccctgagt tgggatgttg
    71521 aatttgtcga tcacgccata gatggtgtcc agcacgcggc ttaagttttc cacgtcgtca
    71581 gtcaccgggt taacgccgat caccgcatcg cccaccccga aggaaagccc ttcgtagatt
    71641 tgcgcggcga tactttgcac gtcgtcacgg gtgtcatttg gctgcaaacg ggcgctgaag
    71701 gtgcccggaa taccgatggt ggtattggcc tttttgatta ccggcatttt cttcgcgccg
    71761 tagatcaggt ccgcgttgga gcaaatcttc gctaccgccg cgaccacttc cgaggtcagc
    71821 cctttgcggg taaaggcaat gtcgtccacg ctggtttcat cgctcagcac atactcacgc
    71881 agttcgctga tgctccagtt tttaatctgg ttgtaggccg tttcgttaac atcgtcctga
    71941 atcagccgcg tcacgcagtc atcttcatag gcaatcaccg gattattgcg gatgtccgct
    72001 acggtcattt ccgacaacac ctgctttgcc gccacgcgct cctgtgagct tgccgctgcg
    72061 acgcccgcca gcacatcccc cgaacgcagt tcgttggctt tagccagcac ctcttttaca
    72121 tccttaaact gatatacatt gccgaacaat gtggtcttta gtttcataag tcgttccctc
    72181 aggaaggaaa tgcgagtgat ttcaccgtca ccggcacaac cgatccgcca aaaagaggcg
    72241 taccaatgtc gatatagtcc cccgcgcgga caatcacttc gtcaatgact gccaacggga
    72301 gttgttgtag ctgtgggcgc aacagcatgc ccagagcttt accaaagtcc tgcccggcca
    72361 ccaccagcag gggatgcgga ttcggaaaac gcgcgacgaa atcgaccaac gcgttgatga
    72421 ccgtcagtac cgcggcgtaa cgcacaggca gcgaggcggg aagcgccagc acgtacgcgt
    72481 cagttttggg atcaagatcc agctgaatca gcgcctgttg ccaggcactc accagatccg
    72541 tttcatcaat cgggatcgcc accggcaaat tgcgcagcgg cagttgtacg ccctccagcc
    72601 agattgtgct gccagagagc gaaagggtat gtgcgcccgc gccaatcacc gtggcgcgca
    72661 cggtttgcgc cggaaactgc acgttcatct cacgcaggcg cggatggtca tgtagcgccg
    72721 tcgccagtag cgggccaata tcggcaaaac agaacgggtc agcgggctgg tggcgataac
    72781 attcgcccac gccgccagaa agcgtaatga tttcgggcgt aacacctgcg ggcagcaaac
    72841 cggtttgcat taatgcctgc gcgagcggcg aaagcgttcc gtcaatcact tcgacaatca
    72901 gctctgccat ccgtcgggta acctgcacca gctgcgcgcc ggtcagcgaa cggacgtcag
    72961 tgcctgcacc gaaacactca tccacaatca tctgccccgg tttatgagcg taaaccacgc
    73021 gcccctggct gtcggtttcc agcaggcgac caccgacgtt gaggcaggca gtgccgctga
    73081 tttttccggc atcgaacagg gcgtagttcg cggtgccacc gccgatgtcg atattcagta
    73141 cccgacacag ccgttgttca gaaagggttt gcgccccggc accgtgaccg gcgatcacgg
    73201 attcgaggtg cggcccggcg ctggcgacga caaaatcgcc cagcgactga gagagcgcca
    73261 tcaccgccgg gcgagcattg cgggttttcg cgctttcacc ggtgatgatg atggcaccag
    73321 aatcaacgct ttccggcgca atgcccgcag cctgatattg ctctaaaatc agcgatttca
    73381 gttccgcttc ttttaaacca ccctgtttat cgacaggggt aaagaacacc gggctttgcc
    73441 agctaatttc gcgtttgatg aattcgtagc gcggcacctg cgacaccgcc gcacggttaa
    73501 ccagttccag gtgggagaaa atcacctggg tggtggtggt gccgatatcg ataccgacgc
    73561 tcagtagctg gcgagtgttc acgattgtgc ctccgcttcg gttttagccg cggtcgcgtc
    73621 gtcttttggc accagcatca tcgccacgcc aatcgccgtt acgccgccga tcaacttgcc
    73681 gacaatcatc gggaagatca tggcattcat gttggcagcg gcgaagccta agtggtcgcc
    73741 cagggcgaaa gcagcggaaa cggcgaaggc gcagttgatg actttgccgc gggtatccat
    73801 ctgcttcatc atgccgaaca tcgggatgtt gttggcaagc gttgccacca tgccggctgc
    73861 cgcgatgttg ttcatattca gcactttacc gacgctcatc agcggttttt caaaccagcg
    73921 ggtcagcagc agcaccatcg gatacgcccc taacagaacg caggagatag aaccgataac
    73981 ttcaatggcg cgcatcacct caccgggttt atcgccaggg gccataaaga taggatccag
    74041 accggggatc agttcccagc caagcaggaa tttcactacc gcagcggcaa gaccgagggt
    74101 gatcaatgca acgaggaatt tggcgaagat ctggaagccg ttgatcattt tttctgggat
    74161 gaatttcagc cccagcgcca ccagaatcgc aacaatgatc accgggatca tgttcatcag
    74221 gatcagggcg aaagtgaatt ccaccggctg gccgttgatc tgaacaccgg agtacatagc
    74281 aaccagacca ccagcgatac aaccaatcgg aatggtcaca atgcccgcca gcacgccgag
    74341 cgccagataa cgacggtcag aaggttcgat aatgccgagc gccaccggaa tggaaaacac
    74401 aatcgttggc cccatcatcg acccgagaat taacccagag tatagccacg cggctacgtc
    74461 gccgcccgcc agctctttgg cgaggaagaa gccgcccata tcgcacgcca gcagtgttcc
    74521 ggcgaacatc gatgggttag cgccgagcat ttcgtaaacc ggaataatta ccggcccgag
    74581 tacgtgagcc agtaccggtg ccagcgcggt cataccgacc atcgccaggc ccagtgcgcc
    74641 cattgccata aagccttctt cgaactgacc gccggatcct tcgatacttt taccgaactt
    74701 accgaggaaa cgagcagaac cgccgaactg tgacaggatc ctgtcaacgg cagctatcag
    74761 cataaagaac atcatgatgt acatgatgat ttcgttaatt cccatcgcct ttactccctg
    74821 ttagttgtta tttattagcg gatgctgcgt gaagccttat ccgcactaca tgtgcaatcc
    74881 cacagaccgg ataagacgcg gcatttattg cgccgctgcg tacaggccga caagctgttc
    74941 caggctggcg gtacgcgggt tgctgcgcag acaaatatct tccagcgcgg cctgcgccca
    75001 tgcaccgtaa tgcgcagatg tcgcgccaac atcgcccagt cgtttaccaa tcccaacttc
    75061 cgcaatcagc tcacttaccg cgttaatagc gtcacgatcg tcggattttt tagttcgcag
    75121 tgcccgacca atctgactaa agcgttcgcg acaaaccatc cggttaaact ccatcaccgt
    75181 tggcagcaac atagcgttcg cgagaccgtg cggaatatgc agcgccgcgc ccggctgatg
    75241 cgccatcgcg tggcacaacc caagacccgc actggaaaac gccattcccg ccatacatga
    75301 agccagcaac atgctctcgc gcgcggcaag gtcgtgaccg tagcccaccg ctttcggcag
    75361 cgatttgcca atcatcgcaa tggcaccaat cgccagactg tcggtaaacg gtgtagcgtt
    75421 cagggcgctg tatgcttcaa tggcatgggt taacgcatca atgccggtca tcgccgtgac
    75481 atgcgacggc acaccttcgg tcaatgcggc gtcgaggatc gccacatccg gcatcagcga
    75541 ggcatgggct aacacctgct tgcgcccgct caccgcgtcg ataatcaccg ttacattggt
    75601 ggtttcagag ccggttccgg cggtagttgg aatggcaatc agcggcaagc gcggttgcag
    75661 aacgctggtt tctgacatct ctgccagcgt gctatccggg ttcgtcacca gcaacgccac
    75721 ggctttcgcc gcatccagca ccgagccgcc gccaaatgcg atcaccccgt cacaacctga
    75781 ctcacgcaac tgcgccacgg ctgcacacac gtcggtaatg cacggttcgc ccaccggaca
    75841 tggccagagc gtcatggcga tacctttaac ggccaggcta cgcgtcagcc cggcggtcat
    75901 ccctgcctga tgcaaaaagc tgtctgccat cacgaacaga tgtttcagcc cacgcgtttg
    75961 cgcttgctgt ccgcaactgc tcaccgagcc cggaccgcaa agcgtcaccg gtggaacgct
    76021 aaatgttttt acccgttgca gattcagggt atcgaacgcc tgaaagagcg cggtctgcaa
    76081 ttcattttgc atatagcccc tccgctttct ctcgcccact actggcgatc gccagcgggg
    76141 tcataaacaa actgtgctgc ggtaaatgca cctgtaacgc cgggaattgt ttgcgaaaca
    76201 gttctgccac gcccggttgc atacaggagc cgcccgccag ccataaatca gtaatgccct
    76261 gcccttcaat atgccgggcg acaatgtccg ccattttttc gtacaccggt ttcaccgtag
    76321 gccaaatctc ttcaccgtga ccgcgcttgt actgctccgc ctcttccagc gaaatacggc
    76381 gatttccggc gaaagtcaga gagatgtgat gtccgccggt ggcttcatcc gccgagtacg
    76441 tcactttgcc ctttttcacg atggcaatgc cggtagtgcc accgccgata tccactacac
    76501 cggcgttgtc cagttgcagc agatccgcga ctgcggttgg ctcatccagc acatggctca
    76561 cttccagccc ggcagactcc agcacgttga tggaaatacg cgggtccgta cctggaggaa
    76621 atgaagtcgc cgcatggcta aaacgacgac cgaattgctg ctcgagcgtg tcgagatggc
    76681 gacgaacaat ggtgaccgcg ccgaagaaat cccagacgat gccgtcgcga accacatcgg
    76741 cccagtcgag gcataccgcc actggctgac cgtcgcggtc gacaaccatc gacaccacat
    76801 cgcaggtgcc cagatccact cccagccaca gcggagattc cgtcgcggcg ggcgtctggt
    76861 tacacagcgt tgccgccgtt tgcagacgtg gggtgagcca ttgttcgtcg tgcgccatct
    76921 gttactcctt aaacaatgcg aaacgcatcg actaatacac agcgacgcag acggacaaac
    76981 gtacgcgcgc tggtcacccc ttcaccggtt ggcgtggtga tggtcatggt ggtccagcct
    77041 tccccgccca gccccagccc ggcaatgcac ggtccgttct taacgaaaat gctggtatca
    77101 atagcattcg ccatctggtt catgttttcg atgttgcgcg agtgcattgc cgccgtgtgg
    77161 tggcaaccgc cttccagttt caccgctagc gcaatggcat ccgccacgtt ggcgacgcgc
    77221 acgacgggca acaccggcat catcagttca gtcacggcaa acggatgttc tgcggtggtt
    77281 tccacaaaca gcaggcgcgt ttcttgcgga actttaaggc cgattgccgc cgcgattttg
    77341 cctgcgtcgc gaccaaccca gtcacggctg acggtgcctt ttccgcgctc gtcgatattt
    77401 ttcagcaaca ccggttgcag ctgctgcgcc tgttctgcgg tcagtttcac cgcgtgctgg
    77461 ccttccatca gacgcatcag ttcatcggct acgctatcaa caacaatcag taccttttcg
    77521 tcggcacaaa tgatgttgtt atcgaaagac gcgcctttga cgatggactg agcagcacgg
    77581 gcgaggtcag cggtttcatc caccactacc ggcgggttgc cagcgcctgc ggcaatcaga
    77641 cgtttattgg tgtgtttacg cgccgcttct actaccgctt cgccgccggt caccaccagc
    77701 aggccgatac ccggaaactt gaacaagcgt tgcgcggttt cgatatccgg atttgccaca
    77761 gtaaccagta agttttccgg cccgcctgcg gcaacaatcg cctggttgag cagcgtgatc
    77821 gcccgctggg agactttttt cgccgccgga tgcggggcaa aaatgacgct gttgcccgcg
    77881 gcaatcaggc tgatggcgtt attaattacg gttgccgccg ggttagtgga cggcgtcacc
    77941 gaagccacca cgccctaggg cgcgttttca attaaagtca ggccgttgtc gcccgtcagc
    78001 acttgcggag agagacactc aacgcctggc gtgccgcgtg cctgagcgac gtttttggca
    78061 aatttatctt caacgcgacc catgccggtt tcactgacgg caagttccgc taaatctctg
    78121 gcgtgttttt cgcctgcttc acgaatggca gcaatggcta actggcgcat tgccacgctc
    78181 tttaaccctt gctgggcaat tttggctgcc gcaacagcgt catccaggga cgcgaaaacg
    78241 cccatctcat gaacggcggc gggcggcgtg tcactgcttt gcattttcag cagtaccgct
    78301 ttcaccacct gttcaatatc ctgttgattc atgatgttct gccttatttg tggaaaatta
    78361 cctgaccgcc agacaccacc tcatcgacga tgccaatcac gcacagatcg accggtgacg
    78421 tttcgctttt atgcgcctgg cgggcggaac tgccactcac cagcaacacc cactccccgg
    78481 ttcccgcgcc aatattgtcg atggcgacgg cacattgccc gtcaggatta ccatgtggat
    78541 caatcatttc caccatcagc aatttgtcat gcgccagtcc gtgatggcgt acggtacaaa
    78601 caatttgtcc agtgacgact gccagtttca tacccgcctc cgtggcgtat ttcaggtaaa
    78661 agctccccct accctccgcg gaaggtaaaa tgaaaaatga gagagcgtga cgcccgaatc
    78721 gacgtcacac agggtgatta caggttgctg ctatcgcctt tcaggccgat cgggaacact
    78781 tcttccaggt cgccgtgcgg acgcgggata acgtgtacag ataccagctc gccgatacgc
    78841 tgtgccgctg cggcaccggc atcggtcgcc gctttacagg ctgcaacatc accacgaacc
    78901 atggcagtac acaggccgcc gccaatctgc ttaacaccaa ccagcttgac gcgcgcagct
    78961 ttcaccatcg cgtcagaagc ctcaatcagt gcaaccaggc cccgggtttc gatcattcct
    79021 aatgcttcca ttgtgctttc ctctttatca gggtccagaa cgggaccgtt cattcaacca
    79081 gtgtttgtaa actgctttcg cggttcactt ctgtctgacg cggcacagct gccaccagcg
    79141 ccagctcgat aatttcctgc acactacaac cacgagagag atcgtgcatc ggcgcggcaa
    79201 gtccttgtat caatggcccg acggcacgat atccgccgag tcgttgtgcg attttgtagc
    79261 caatatttcc ggcttccagc gacggaaaaa ccatcacatt ggccttgccc tgtagcgggc
    79321 tggcaggcgc tttttgcgcc gccacttccg gcacgaaggc ggcgtcaaac tgcaactcgc
    79381 catcgactac cagctttggt gcgcgctcac ggacgatttc tgtcgcctgc tggacattgg
    79441 caacgcaggg gtgacgggcg ctaccgttac tggaaaacga cagcatcgcc acgcgcggct
    79501 cttctccggt gatggcgcgc caggtttcgg cgctggcaag cgcgatatcc gccagctgcg
    79561 ccgccgtcgg ctgtggtacc acgctgcaat cggcaaagcc caacgccgga ccgctgtact
    79621 gtggcagcat caggaaaatg gatgagagcg ttttacagcc tggctgcaag ccgataatgc
    79681 gtaatccggc acgcagcaca ttcgccgtgg aagagaggtt gcccgcgata cagacatccg
    79741 ctttaccggc gctgaccatt gcggcggcga acatcagcgg atcggtaagt ttttccagcg
    79801 catccggcgg cgttttttcg cccgcgcggg ccagccagcg atgagcaaat tcttcccgca
    79861 ttgcgaggtt gccatgcgga tctatcacct gtagcccgtc catcgccacg ccgtgactga
    79921 gcgcaaactg acgaagttca aacggattgg cgaccagaat gggcgttgcc agaccttgtt
    79981 gatgtaaata ttgcgcagct ttcagcacac gttgatctaa cgcatccgga aaaaccactc
    80041 tggcgggcgc tcgcaacgcc agttcacaac aacgttcaat aatcattgcg tttcccccag
    80101 tcgttgttgg atctgcttca ccgttagcgg ctgcttcgtt acgctcagga tcatcatcac
    80161 gtagaccgtg ctggaaaggc ggttcagcgc ctgcaagatg tccgggcgta atacttcaaa
    80221 actgcgggtg ataaacacct gcgccgctac ggtttcggct tcccgcactt tggtgcgtag
    80281 cagattcagc aacgccgcat cgcgcccgtg gcttgcttcc ggcaccagat gatcgtgacc
    80341 aagataacgc aacggctgat gagaaagccg gtgcagatct tcgtcagaaa gcccgacaat
    80401 cgcctggcag cccagcggtt ccgccagttc aatttgcagc cacactgcca gcgcaatggt
    80461 gctgtcgaga actgcgcgaa aacccagacg cggatcgctt ttggcgacca ttttttccgc
    80521 cgacaggtga gtcagcgtat ccggcttttt tgccaccggc tggcggcaca gttcgcagca
    80581 cgcctgcgga tgttcatcgc tactggttag cccatgaacg ggctgcggct gctgctgttc
    80641 atcgtcaaca aacaggcggc cctgctcgtc aataaacttg atgcgcagat ggcggctttc
    80701 cagcaactcc cgggcagagg gcgtcaggcg actgtccgca gggagatgga tctctgcgcc
    80761 ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg atgaaatctt tcatagggat
    80821 tgccagttag ccggccaggc gacatacagg aatttcacgc tggatgtcgt accaaattcg
    80881 atgctggagc ctttcgggat aaacatcacg tcgcctgctt tagcgatcat ggtttcgcct
    80941 tcgtggcgaa catggagctc cccttccagc accatgtcga tttcgtcgta gttcagggtc
    81001 cacgggaaga atgcgttctc ccactgcata aaaccggcgg ccatgctgct gccgtcgtct
    81061 ccggtcacaa gatcagtcaa gcccacgcaa tgcggttgtg cgccatcaaa gcgaccaaac
    81121 ttgacgctgc taccatcaat cactttgatg ccgcctttgc cggtcaccga tttgaagctc
    81181 ggctgcattg caccctgctc cagcgactgt ttttccttca tcactttttc catcagctgc
    81241 gcgaccaggc tttcggtaaa ctggccttcc ggtagctggg cgataatggt ttcgcggatg
    81301 cgctggcttt cggttttatc ggctggcacg ctggcgggca cggacgccgt taccgggatc
    81361 aattcatcac attcggtgat ggtaaaaccc agtagatccg ccacttcacg ggcttccggg
    81421 gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca
    81481 cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg tgagagatgc
    81541 cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg
    81601 ctcctcgaag cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag catccggcat
    81661 gtccgtttta ctgatgacgg cgatttgccg tttgctgacg ccaatatcca gcaacccggc
    81721 aggcaaccga ctttccgggt cattcgcgcc gtggacatag atcagcatgt ccacatcctg
    81781 tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac ccggcgtatc
    81841 aatatcgccc ttatcattaa attccacggc ctgtgttttt ctggcgaggg tataattccc
    81901 ctgcaacgca ttaaataacg ttgttttccc tgccccgacg gtacccacaa acgcaattcg
    81961 tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat ttaataagcg
    82021 acctaaacca ctgacggttt gtgataacgc ttcttctact gcgcccaccg agccataaat
    82081 caccaatgca ccactgaagc ggtcgagaaa tccgatatgc acatcggcgg ctttcagcgc
    82141 taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc caattgcgcc
    82201 cgcatccgga acgccgattt tcttcgccag ttcctcgccg ggatgcgcga tgagatgcgc
    82261 cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat ccatgagtcg
    82321 ccgcctgtga aaaactttcg tgtatcgtga caatttttcg tcagagtaaa taacaaaatt
    82381 cggcaagcgc gtttaaaagg tgaggtagat cactaagaat ggagagatat ttactgttat
    82441 gaataaactt tcatatgcga cgcatgaata taaataaaat aatatcgaga tattttatat
    82501 actgcttttt ttattattcg caaactcagt aataagcttt actaatatat tgcagctaaa
    82561 ccgcttaccc tgaatattca gggtaagcgt gagagttaaa aaagaattac agcggttggg
    82621 tttgcgcttc aaccacggcc agcgccacca tgttgacgat acgacgcacc gatgcgatcg
    82681 gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca gtcacacctt
    82741 ccgagctgga aacgcgcagt aagttgtaac taatgcgggc agcttccatg ttcggcatca
    82801 ccagaatatt ggcagaacct ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt
    82861 ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagttctggc gcacgttccc
    82921 tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt
    82981 tggagtgcga caacagagca acgcgcggtt caataccgaa acgacggaca gtttctgccg
    83041 ccatcaaggt gatctccgcc agctcttctg cgtctggttc gtcattgacg taggtatcgg
    83101 caataaaggt gttaccactc ggcagcagca gcgcgttcat tgcacctgcg gtgtgaacgc
    83161 catcgcgata accaaagaca tttttcacca cgctaaaatg ttcatgataa tcacccaccg
    83221 taccgcaaat cattgcatcg gcttcgccac gctgaaccat gatcgcgccg atcactgtcg
    83281 ggttactgat cagcgcccgc tgcgcctgtt cctgagtgac gccgcgacgc ttcatgatct
    83341 ggaagtattc ggtccagtac tctttaaagc gcggatcgga ttcgttattg acgatctcaa
    83401 aatcaacgcc cgctttgatc tgcaagccca gtttctgaat gcgcatttcg atcacgttcg
    83461 gacgaccgat aaggatcggt ttcgccagtc ccagcgttac cagttcctga gtggcatgca
    83521 gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt cggcgctttg cgagcctggg
    83581 agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctcagtcagc ttgtcgatgt
    83641 agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc gctttcgcga
    83701 ccgcaggagc aatcttgact atcagacgcg gatcaaacgg ttttggaatg atgtattcag
    83761 ggccaaagct cagatcctga tcgccatacg ctgaagccac cacttcactc tgttccgcat
    83821 gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg atggcggttg
    83881 cgccaacgtc cagcgcgcca cggaagatga acgggaagca aaggacgttg ttcacctggt
    83941 tcgggtagtc ggaacggccg gtacagatga tggcatccgg acgcacttct ttcgccagcg
    84001 gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggcgct cgagccattt
    84061 tcttcaccat ttcctgggtc agcactttcg ggccggaaca gccgaggaaa atatctgcgc
    84121 cttcaatcac atcatcgagg gtacgtttgc cgtcatccac taccgcatat gcggctttgg
    84181 tttccgccat gtttggctca cggccctgat agataacgcc ttttgaatcg caaaccacga
    84241 tgttatgttt ttgcagaccc aacgctacca gcaggttcat acaggcgatt gctgcagcac
    84301 ccgcgccgga aaccaccata cgcacgtcag agatgttttt ctccaccacg cgcaagccgt
    84361 tgaggatggc ggcagtgctg ataattgccg tgccgtgctg atcgtcgtgg aataccggaa
    84421 tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt
    84481 cgaggttgat gccgccgaag gtcggttcga gcgcagcgac gacttcaata aatttgtccg
    84541 ggtcgagttc atcaacttca atgtcaaata catcaatccc agcgaatttc ttaaacagaa
    84601 cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct aaccccagca
    84661 ccgccgtacc gttagagatc accgccacca ggttaccgcg cgcggtatat ttgtaggctt
    84721 ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag taggccagcg
    84781 ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc cctggaactg
    84841 gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg ttgttccttt
    84901 cacgtaacgt tcacaaataa agtgtgtggg caacagcccc tgcccacaac gtggcgcaca
    84961 ttattacctt gccggagtct acagactttg agcaagtcca aactctcacc attaatataa
    85021 tgttttggta ataatcctat aacactgatg ttacctgctt aatccagcaa taccatgcct
    85081 gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact
    85141 catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg gcatcaaaca
    85201 gttcaccact gtcgtggcag acagcggcga tattgagtcc attcgccatt atcatcccca
    85261 ggatgccacc accaatcctt cgctgttact caaggctgcc ggattatcac aatatgagca
    85321 tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg aacaacaggt
    85381 ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaatcctca aaatcgtacc
    85441 cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa agagtattga
    85501 gaaggcgcgc catctggtgg acttgtatca gcaacaaggc gttgagaaat cacgcattct
    85561 gatcaaactg gcttcgacct gggaaggaat tcgcgcggca gaagagctgg aaaaagaagg
    85621 tattaactgc aacctgacgc tgctgttttc ttttgcacag gcgcgggcct gtgcagaagc
    85681 gggcgttttt ctgatttcgc cgtttgtcgg gcgtatttat gactggtatc aggcacgcaa
    85741 gccgatggac ccatatgtgg tggaagaaga tccgggcgtt aaatcggtgc gcaatatcta
    85801 cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct tccgtcgcac
    85861 cgaacaaatc ctcgccttaa ccggctgcga tcgactgact atcgcaccga atttgctgaa
    85921 ggagctgcag gaaaaagttt cgccagtggt acgtaaatta atcccacctt ctcagacgtt
    85981 cccacgccca gctcccatga gcgaagcgga gttccgttgg gagcacaatc aggatgcgat
    86041 ggcggtagaa aaactgtctg aaggcattcg tctgttcgcc gttgatcaac gcaaactgga
    86101 agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc cgaaaagacc
    86161 ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca aaaagccaat tctggtcatc
    86221 ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat tttcttaaac
    86281 ataaccctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc aacggtcacg
    86341 cgtcgatgct gctctacagt ttgctgcatc tgaccggtta cgacctgccg ctggaagagc
    86401 tgaagaactt tcgtcaactg cattcgaaaa cccctggcca cccggaaatc ggctatacgc
    86461 ccggagttga aaccaccacc ggtcctcttg gacaaggttt ggcgaacgcc gtcgggctgg
    86521 cgatagcgga acgtacactg gcggcgcagt ttaaccagcc ggatcatgaa attgtcgatc
    86581 acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatttcc cacgaagtct
    86641 gttcgctggc gggcacgctg ggactgggca agctgattgg tttttacgat cacaacggta
    86701 tttcgattga tggagaaacc gaaggctggt ttaccgacga tacggcaaaa cgttttgaag
    86761 cctatcactg gcatgtgatc catgaaatcg acggtcacga tccgcaggcg gtgaaggaag
    86821 caatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc cgtacggtga
    86881 ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca ccgctggggg
    86941 aagaagaagt ggcgctggca cggcaaaaac tgggctggca ccatccgcca tttgagatcc
    87001 ctaaagatat ttatcacgct tgggatgccc gcgaaaaagg cgaaaaagcg cagcagagct
    87061 ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa gagtttaccc
    87121 gtcggatgag cggtggttta ccgaaggact gggagaaaac gactcagaaa tatatcaatg
    87181 agttgcaggc gaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat acgcttaacg
    87241 cttacgggcc gatgctaccg gagctgctcg gcggttcggc ggatctggct cccagcaacc
    87301 tgaccatctg gaaaggttct gtttcgctga aggaagatcc ggcgggcaac tacattcact
    87361 acggggtgcg tgaatttggc atgaccgcta tcgccaacgg cattgcgcac cacggcggct
    87421 ttgtgccgta taccgcaacg ttcctgatgt ttgttgaata cgcccgtaac gccgcgcgga
    87481 tggcggcact gatgaaagcg cggcagatta tggtttatac ccacgactca attggtctgg
    87541 gcgaagatgg tccgacgcac caggctgttg agcaactggc cagcctgcgc ttaacgccaa
    87601 atttcagcac ctggcgaccg tgcgatcagg tggaagcggc ggtgggctgg aagctggcgg
    87661 ttgagcgcca caacggaccg acggcactga ttctctccag gcagaatctg gcccaggtgg
    87721 aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg
    87781 gcggtaagcc agatattatt ttgattgcca ccggttcaga gatggaaatc accctgcaag
    87841 cggcggagaa attagcggga gaaggtcgca atgttcgcgt ggtttccctg ccctcgaccg
    87901 atattttcga cgcccaggat gaggaatatc gggagtcggt gttgccttct aacgttgcgg
    87961 ctcgcgtggc ggtggaagca ggtattgccg attactggta caagtatgtt ggtctgaaag
    88021 gggcaattgt cgggatgacg ggttatgggg aatctgctcc ggcggataag ctgttcccgt
    88081 tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtgctg ggagtaaaag
    88141 gtgcctgatg gtgatcgccg gatgctgatt gccggatgcg acgctgacgc gtcttatccg
    88201 gcctacagcg tcttatccgg cctacatgtc ccgccatttt gtttaacggg tgatccacaa
    88261 cgtgggccag gcgtctggcc catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac
    88321 caggcgaaaa cgctgaccgt caaagcgcca gcgcgcctga atgccacaat cgcttaatcc
    88381 gcgccctttc gctaaggtca ccagttcacg cgatttctca tcaaatgttg cgttcatcag
    88441 ttccagttca ttcgtctcct gaccgttgtt gaacggcaaa cgcagccgga ccgggcgcga
    88501 ggccagcggc tttttacgcg acacaatcca tgccaaatca atggtgttat aggcccctgc
    88561 ctcacagcta atcatcatca gcgctttatc atcagtcagc gcagtgacat tcacctcacg
    88621 acgcaatgga tcaagcgagc agcgcagacc attcatccgc cagtttccat aatccagcaa
    88681 atcgttgcgt tcttcgagtg agagtggcgt cggcgttggg ttaaccacca cgacctcttt
    88741 cagcgcaggc gcgggcggta cgctgagcgg cggttcgtcc cctttcttga tccacgcggt
    88801 ttcactgcca acgcgctttt gctgagcatc aataaacaac aacgccgctt ttaagtcact
    88861 cagagaaatg gtctgattgc cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg
    88921 caaaaacgcg gtgatggttg ccgtatcatc ggttactaat aaccatggtg aaatccgcca
    88981 cttgtcacca cttaacgcca acggctcgcc atctaacagc agccgtggcg caatttcccc
    89041 ttcagacgca tccggcgact tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg
    89101 cgccccggcg ctgcggctca gggtcatcac cagtccatta tgatcgcccg tattacgcgc
    89161 cacgcagaag ttttggttat tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg
    89221 tgccgcccac accaaagacg tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata
    89281 gcgcatggac ggtacgaccc cagaagttca aacaaacagc acaagtcgta tcttcgttgc
    89341 gcaggtagcg cggctcaatc ggatttgtcg gattgagcca agaataaaca acgaaatatt
    89401 attgttattt ttcagtctat taaatgtgac atttgcaaat agttaagcaa taacaccgtc
    89461 ttaccgtcac gtatctcgcc ggttttgatc atctccagcg cctggctgaa cggcagctcg
    89521 agcacttcaa tatcttcatc ttcgacaccg ccaccggcgt tagcgcgctg attgtcactg
    89581 tattcggcga taaaaaagtg gattagctca gtcacaccgc ctggcgacat atacagttca
    89641 aataatttgc gcacttcgcc cacttcatag cccgtctctt caatcgcttc tttgcgaatg
    89701 cacacttccg gttcgtcgtt atccagcagc ccggcgcagg tttcaatcag ctgcccgctt
    89761 tcattgccat taacccaggt agcgacacgg aactgacgaa tcagaaccac ggtctttttc
    89821 ttcgcgttgt acaggaggat cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg
    89881 cggataactt cgccatcttt gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag
    89941 ttatcggaga gaattttgtc tttaatgagg gtgatttgtt gcgtcatacc gactccacag
    90001 cgcgaaatga acaattatat tacgctgtga agtcgggttt gtctgcgcag gctatcagtg
    90061 agatttgact gattttaccc ccagccagtc aataatcccc tgtgccgcat gacgtccttc
    90121 tgccattgcg gtaaccacca gatccgcacc acgcacggcg tcgccaccgg cgaagatttt
    90181 cggattggtg gtctggtaac ggtactggct ttccacatcc gcgatgatgc ggccccattt
    90241 gtctaccgtt acaccgtgcg actccagcca cggcatcccg tgcggattga agccaaacgc
    90301 cataatcacc gcgtcggctg gcatgacaaa ttcactgcct tccaccggca ctggacgccg
    90361 acgcccctgg gcatccggct cgccaagacg cgtgcgcagg aaacgaatcc cgcagacgtg
    90421 accttgttca ttcagctcaa gcgccaccgg ctggacgtta aattcgaagt tggccccctc
    90481 ttcgcgggcg ttcttcacct ctttcttcga gcctggcatg ttagcttcat cacgacgata
    90541 agcgcaggtg acgttactcg cgccgtggcg cagcgcagta cgcacacagt ccatcgcggt
    90601 gtcgccgccg cccagtacca cgacgttaag tccggcggta ttgataaacg gctcttccgg
    90661 tagctcttcg agccccatca cctgtttagt gttggcgatg aggaacggca gcgcgtcata
    90721 aacgcccggc gcatcttcat tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc
    90781 aacgaagacc gcgtcgtatt gttccaaaag cgaatccaaa gagacatctt tacccacttc
    90841 acaattgagt tcgaagtgaa tccccatcgc gctgaagatt tcccgacgac gtgccagcag
    90901 ggatttatcc agtttgaaag aaggaatgcc gaaagtgagc aagccaccga tttctggatg
    90961 gcgatcgtac accgtcaccg ccacgccatt gcggatcaga acatccgcac aggccagccc
    91021 tgccggacct gcaccgataa tcgccacccg cttgtccact ttggtgacat ggcttaagtc
    91081 aggacgccaa cctttcgcca acgcctgatc tgaaatgtag cgttcaatgt tgccgatagt
    91141 taccgcaccg tgctcatcgc gaatagtaca ggcaccttca cacaaacggt cttgcggaca
    91201 aacgcgtccg gtaatttccg gcagggtgtt ggtcaggtga gaaagctcga cggcggcgtc
    91261 gatgtttccg gctttcacca gttcaatcca ctgcggtata tggttatgca gcgggcaggt
    91321 ccattcacaa acgctgtgct cgccgcattt aaggcagcgc gaggcttccc gttgtgcctg
    91381 atcggcacgg aatggcagat aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag
    91441 tttatccggc tcgccacgcg cgggcgttgc ctgcatttgt tcgactttac tcattaccgg
    91501 catttcttgt gcagcggtac tggcatgcca cggttgatgt tcctgacgtg cggtacgcaa
    91561 gcggcgggat ttcgccatgc ctgagagtgc ggcgtcagtg accagctgca gcgcgtccgc
    91621 cgggcaattc tcgacacagg caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg
    91681 cgccgtggct ttaactttgc ccgctgcgac gggtgtcagg acgatttgca tcgtaccaaa
    91741 cggacaggcc accacgcagg atttacagcc aatacacttt tgctgattga cctgaatgct
    91801 gtcatcaacg tggctgattg cgccattagg gcagctacgg gcgcagggcg catcttcaca
    91861 atggtgacag gtcactgcac tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg
    91921 aaaatggtgt tggctcagga catgttgctc atcattgtga gccatgacac aagcgatttc
    91981 acaagcatga caacccagac attgctgact gttggccata ataaaacgat tcataacgac
    92041 cttctttttt ggttgtaaaa accttattct ttatatgagt gttgttatta cccgacttac
    92101 aggggaatcg gcaatgttca tgtgcccaga ataagtaact atttcgctat aaactgtggc
    92161 agatcaaata atcccatcac taactaaatt gcgtttcagc gaactggaac attaatgatt
    92221 ttttgtggag aagacgcgtg tgattgttaa acgacccgtc tcggccagtc tggcccgggc
    92281 ctttttttac attgtgctgc tgtcgattct ttccacgggt atcgctctgc taactctggc
    92341 gagcagtttg cgcgacgctg aggctatcaa tattgccgga tcgctgcgta tgcagagtta
    92401 ccgcctgggc tacgacttgc aaagtggcac tccacaactc aatgcacatc gccagctgtt
    92461 tcagcaggca ctgcattcac cggtattaac caacctcaac gtctggtatg tgccagaagc
    92521 agtaaaaact cgctatgcgc atctgaatgc caactggctg gagatgaata atcggctcag
    92581 caagggcgat ttgccgtggt atcaggccaa tattaataat tatgttaatc agatagacct
    92641 gttcgtgctg gctttacagc actacgctga acgcaaaatg ctgctggtgg tggcgatttc
    92701 cctggctggc ggtatcggta ttttcacgct ggtctttttt actttgggcc gcatacgcca
    92761 tcaggtggtt gccccgctga atcagctggt taccgccagt cagcgtattg aacacggaca
    92821 gttcgactcg ccaccgctgg ataccagcct gccgaatgag cttggtctac ttgcaaaaac
    92881 ctttaaccag atgtcgagcg agctgcataa attgtaccgt tcgctggagg cgtcagtaga
    92941 agaaaagacc cgcgatctcc acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc
    93001 gcaggcgctg aacaccagcc agattgatgt gcattgtttc cgccatattt tgcagattgt
    93061 tcgcgacaat gaagcggctg aatatctgga gttaaatgtc ggtgaaaact ggcggattag
    93121 cgaagggcaa ccaaacccgg aattgccgat gcagatttta ccggtgacaa tgcaagagac
    93181 ggtttacggc gaactgcact ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa
    93241 cagcgtttcg tcgatgctgg gacgcggttt gtactttaat caggcgcaga agcattttca
    93301 gcagttattg ttgatggaag aacgtgcaac catcgcccgc gaattgcacg actcgctggc
    93361 tcaggtactt tcttacttac gtatccagtt gacgttactt aagcgttcga taccggaaga
    93421 taacgccacc gcacaaagta tcatggccga tttttcccag gcgttgaatg atgcttatcg
    93481 gcagttacgc gagctgttga ctacttttcg cctgacgctg cagcaggcgg atctcccctc
    93541 cgcgttgagg gaaattctgg atacgttaca aaatcaaacc agcgccaaac tgaccctcga
    93601 ctgccgtctg ccaaccctgg cactggatgc gcaaatgcag gtgcatttgt tgcaaattat
    93661 tcgcgaagcg gtgctgaatg cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg
    93721 cgtcaccgcg ccggacggca atcacacggt ttatatccgt gataacggga ttggtatcgg
    93781 tgaaccgaaa gaacccgaag gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg
    93841 gctaggtggg acgctgactt tttcacaacc ttccggcggc ggcacgttag tgagtattag
    93901 ctttcgctct gcggagggtg aggaaagtca gttgatgtaa tgcctcctac tgaccaaaga
    93961 atacttgcag ttaaggttca gtataaaagg gcatgataat ttacattaac tccttttttt
    94021 ctccacgatt ggctcgtacc ttgccgctac agtgaagcaa gtcaagccta caacgatacg
    94081 cagaaacacg aggtcctctt ttaatggcga atttctttat tgatcgcccc atttttgcct
    94141 gggtgctggc aatcctgttg tgtctgacag gtaccctggc gattttttca ttgcccgttg
    94201 aacaataccc cgatctcgcg ccaccgaatg tgcgagtgac cgctaactat cccggcgcat
    94261 cggcccagac gctggaaaac accgtgaccc aggttatcga gcaaaatatg accggcctcg
    94321 ataatctcat gtatatgtca tctcagagca gtggcaccgg tcaggcatct gtcactttaa
    94381 gttttaaagc aggcaccgat ccggacgaag ccgtgcagca agtacaaaac cagctgcaat
    94441 cagccatgcg aaagttaccg caggcggtgc aaaatcaggg cgtgactgtg cgtaaaaccg
    94501 gcgataccaa cattctgacc attgccttcg tctctaccga tggttcgatg gataaacagg
    94561 atattgctga ttatgttgcc agtaatattc aggacccgtt aagccgcgta aatggcgtcg
    94621 gggatatcga tgcctatggt tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac
    94681 tcaacagttt ccagatgaca gctaaagatg tcactgatgc cattgagtca cagaacgcgc
    94741 agattgcggt tgggcaactt ggtggtacac cttccgtcga taagcaggcg ctcaacgcca
    94801 ccattaacgc ccagtcactg ctgcaaacac cagaacagtt ccgcgatatc accttgcggg
    94861 tgaatcagga cggctcagaa gtaaggctcg gcgatgtcgc caccgccgaa atgggggtgg
    94921 agaaatacga ttatcttagc cgcttcaatg gtaagccagc ctccgggttg ggggtaaaac
    94981 tggcctccgg cgcgaacgaa atggcgacag cggagctggt gctcaatcgt ctcgacgagc
    95041 tggcgcagta tttcccgcac ggactggaat acaaggtggc gtatgaaacc acctcgtttg
    95101 ttaaagcctc cattgaagac gtggtgaaaa cgctgctgga agctatcgct ctggttttcc
    95161 tcgttatgta tctgttcctg caaaacttcc gcgccacgct gatacccact atcgccgtgc
    95221 cggtggtgtt gatgggaacc ttctccgtac tttacgcctt cggttacagc gtcaacacct
    95281 taaccatgtt cgcgatggtg ctggcgatcg gtctgctggt ggatgacgcc atcgtggtgg
    95341 tggaaaacgt cgaacgtatt atgagtgagg aaggactcac tcctcgcgaa gccacgcgta
    95401 aatcgatggg gcagatccag ggggcactgg tcgggattgc gatggtgctt tcggcggtat
    95461 ttgtaccaat ggccttcttc ggcggcacca ccggtgccat ctatcgccag ttctctatta
    95521 ccattgttgc ggcgatggtg ctgtcagtac tggtagcgat gatcctcact ccggctctgt
    95581 gtgccacact acttaagcca ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg
    95641 cctggtttaa ccagatgttt aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa
    95701 ttctccaccg tagcctgcgc tggattgtga tttatgtcct gctgcttggc ggcatggtgt
    95761 tcctgttcct gcgtttgccg acgtcgttct taccgctgga agaccgtggc atgtttacta
    95821 cctcggtaca attgcccagc ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa
    95881 tcgagaaata ttacttcacc catgaaaaag acaacatcat gtcggtgttt gccactgttg
    95941 gttctggccc tgggggtaac gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact
    96001 ggagcgaacg cgacagtaag accggcacct cgtttgccat tatcgagcgt gcaacaaagg
    96061 cgtttaacca aattaaagaa gctcgcgtta tcgccagcag cccgccagca attagcggtc
    96121 ttggtagttc tgcaggtttt gatatggagt tgcaggacca cgctggagca ggtcacgatg
    96181 cgctgatggc agcacgtaac cagttgctgg cgctggcggc ggaaaacccg gagctaaccc
    96241 gtgtgcgcca taacggcctc gatgacagtc cgcagttgca gattgatatc gaccagcgta
    96301 aagctcaggc gctgggcgtt gctatcgacg atattaacga cacactgcaa accgcctggg
    96361 gttcgagcta tgtgaatgac tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg
    96421 cagctgcgcc gtatcgcatg ctgccagatg acatcaatct ctggtatgtc cgaaataaag
    96481 atggcggcat ggtgcccttc tctgctttcg cgacctcacg ctgggagaca ggctcgccgc
    96541 gtctggaacg ctataacggt tattctgcgg ttgaaattgt tggggaagcc gcaccggggg
    96601 tcagtaccgg tacggcgatg gatattatgg aatcgttagt gaagcagcta ccaaacggct
    96661 ttggtctgga gtggacggcg atgtcgtatc aggagcggct ttccggcgcg caggctccgg
    96721 cgctgtacgc catttccttg ctggtggtat tcctgtgtct ggctgcattg tatgaaagct
    96781 ggtcggtacc gttctcggta atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg
    96841 caacctggat gcgcgggctg gaaaacgacg tttacttcca ggtgggcctg ttaacggtca
    96901 ttggtttatc ggcgaaaaac gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa
    96961 aaggccacga cctgtttgaa gcgacgctcc acgcctgccg tcagcgttta cgcccgattc
    97021 tgatgacctc gctggcattt atcttcggcg tattgccaat ggcaaccagc acgggtgccg
    97081 gttccggtgg tcagcatgcg gtgggtactg gcgtaatggg cgggatgatt tcagccacta
    97141 ttctggctat ttacttcgtg ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga
    97201 agccgcgccc ggaataagca ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact
    97261 ttccgattaa gaacctgctc agcgggttct tgctgtttgt actttgtctc aggaattact
    97321 tacgaagcat aacttcaata aagtctttcc agttccccag ttcacgttca atcataacaa
    97381 cctctcttat aattatgggt attctacgga aacaatatac cgtggtgaag ctaatttact
    97441 cgattgctgc gatgactacc tccgggggac aaaccttatg taaatactat ggtcctgcag
    97501 tgatgatttg tatgtgatac acagcaacat ttcgagatat tcatacggca tctaatactg
    97561 atttaattct ggttaaaata
//
LOCUS       DAZFTN010000006        95054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFTN010000006 DAZFTN010000000
VERSION     DAZFTN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1178
                     /gene="proV"
                     /locus_tag="ACHKV2_000509"
     CDS             <1..1178
                     /gene="proV"
                     /locus_tag="ACHKV2_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO6938457.1"
                     /translation="LYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEE
                     GEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMV
                     FQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPNELSGGM
                     RQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDE
                     AMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSAKDIARRTPN
                     GLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAAL
                     IDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1171..2235
                     /gene="proW"
                     /locus_tag="ACHKV2_000510"
     CDS             1171..2235
                     /gene="proW"
                     /locus_tag="ACHKV2_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO6938458.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2292..3284
                     /gene="proX"
                     /locus_tag="ACHKV2_000511"
     CDS             2292..3284
                     /gene="proX"
                     /locus_tag="ACHKV2_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO6938459.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3476..4660
                     /locus_tag="ACHKV2_000512"
     CDS             3476..4660
                     /locus_tag="ACHKV2_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6938460.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4784..5521
                     /gene="ygaZ"
                     /locus_tag="ACHKV2_000513"
     CDS             4784..5521
                     /gene="ygaZ"
                     /locus_tag="ACHKV2_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO6938461.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5511..5846
                     /gene="ygaH"
                     /locus_tag="ACHKV2_000514"
     CDS             5511..5846
                     /gene="ygaH"
                     /locus_tag="ACHKV2_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO6938462.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5937..6467
                     /gene="emrR"
                     /locus_tag="ACHKV2_000515"
     CDS             5937..6467
                     /gene="emrR"
                     /locus_tag="ACHKV2_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO6938463.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6594..7766
                     /gene="emrA"
                     /locus_tag="ACHKV2_000516"
     CDS             6594..7766
                     /gene="emrA"
                     /locus_tag="ACHKV2_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO6938464.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7783..9321
                     /gene="emrB"
                     /locus_tag="ACHKV2_000517"
     CDS             7783..9321
                     /gene="emrB"
                     /locus_tag="ACHKV2_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO6938465.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9385..9900)
                     /gene="luxS"
                     /locus_tag="ACHKV2_000518"
     CDS             complement(9385..9900)
                     /gene="luxS"
                     /locus_tag="ACHKV2_000518"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO6938466.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10050..11606)
                     /gene="gshA"
                     /locus_tag="ACHKV2_000519"
     CDS             complement(10050..11606)
                     /gene="gshA"
                     /locus_tag="ACHKV2_000519"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO6938467.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11679..12107)
                     /gene="yqaA"
                     /locus_tag="ACHKV2_000520"
     CDS             complement(11679..12107)
                     /gene="yqaA"
                     /locus_tag="ACHKV2_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO6938468.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12104..12670)
                     /gene="yqaB"
                     /locus_tag="ACHKV2_000521"
     CDS             complement(12104..12670)
                     /gene="yqaB"
                     /locus_tag="ACHKV2_000521"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO6938469.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12951..13027)
                     /locus_tag="ACHKV2_000522"
     tRNA            complement(12951..13027)
                     /locus_tag="ACHKV2_000522"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12991..12993),aa:Arg,seq:acg)
     gene            complement(13130..13206)
                     /locus_tag="ACHKV2_000523"
     tRNA            complement(13130..13206)
                     /locus_tag="ACHKV2_000523"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13170..13172),aa:Arg,seq:acg)
     gene            complement(13269..13345)
                     /locus_tag="ACHKV2_000524"
     tRNA            complement(13269..13345)
                     /locus_tag="ACHKV2_000524"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13309..13311),aa:Arg,seq:acg)
     gene            complement(13410..13486)
                     /locus_tag="ACHKV2_000525"
     tRNA            complement(13410..13486)
                     /locus_tag="ACHKV2_000525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13450..13452),aa:Arg,seq:acg)
     gene            complement(13490..13582)
                     /locus_tag="ACHKV2_000526"
     tRNA            complement(13490..13582)
                     /locus_tag="ACHKV2_000526"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13546..13548),aa:Ser,seq:gct)
     gene            complement(13898..14083)
                     /gene="csrA"
                     /locus_tag="ACHKV2_000527"
     CDS             complement(13898..14083)
                     /gene="csrA"
                     /locus_tag="ACHKV2_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO6938470.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14318..16948)
                     /gene="alaS"
                     /locus_tag="ACHKV2_000528"
     CDS             complement(14318..16948)
                     /gene="alaS"
                     /locus_tag="ACHKV2_000528"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO6938471.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17076..17576)
                     /gene="recX"
                     /locus_tag="ACHKV2_000529"
     CDS             complement(17076..17576)
                     /gene="recX"
                     /locus_tag="ACHKV2_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO6938472.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17644..18705)
                     /gene="recA"
                     /locus_tag="ACHKV2_000530"
     CDS             complement(17644..18705)
                     /gene="recA"
                     /locus_tag="ACHKV2_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO6938473.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18785..19282)
                     /gene="pncC"
                     /locus_tag="ACHKV2_000531"
     CDS             complement(18785..19282)
                     /gene="pncC"
                     /locus_tag="ACHKV2_000531"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO6938474.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19427..20512)
                     /gene="mltB"
                     /locus_tag="ACHKV2_000532"
     CDS             complement(19427..20512)
                     /gene="mltB"
                     /locus_tag="ACHKV2_000532"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO6938475.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20767..21329
                     /gene="srlA"
                     /locus_tag="ACHKV2_000533"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             20767..21329
                     /gene="srlA"
                     /locus_tag="ACHKV2_000533"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            21326..22287
                     /gene="srlE"
                     /locus_tag="ACHKV2_000534"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             21326..22287
                     /gene="srlE"
                     /locus_tag="ACHKV2_000534"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            22298..22669
                     /gene="srlB"
                     /locus_tag="ACHKV2_000535"
                     /gene_synonym="gutB"
     CDS             22298..22669
                     /gene="srlB"
                     /locus_tag="ACHKV2_000535"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO6938476.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22673..23452
                     /gene="srlD"
                     /locus_tag="ACHKV2_000536"
     CDS             22673..23452
                     /gene="srlD"
                     /locus_tag="ACHKV2_000536"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO6938477.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23558..23917
                     /gene="gutM"
                     /locus_tag="ACHKV2_000537"
     CDS             23558..23917
                     /gene="gutM"
                     /locus_tag="ACHKV2_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO6938478.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            23984..24757
                     /gene="srlR"
                     /locus_tag="ACHKV2_000538"
                     /gene_synonym="gutR"
     CDS             23984..24757
                     /gene="srlR"
                     /locus_tag="ACHKV2_000538"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO6938479.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            24750..25715
                     /gene="gutQ"
                     /locus_tag="ACHKV2_000539"
     CDS             24750..25715
                     /gene="gutQ"
                     /locus_tag="ACHKV2_000539"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO6938480.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25712..27226)
                     /gene="norR"
                     /locus_tag="ACHKV2_000540"
     CDS             complement(25712..27226)
                     /gene="norR"
                     /locus_tag="ACHKV2_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO6938481.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27413..28852
                     /gene="norV"
                     /locus_tag="ACHKV2_000541"
     CDS             27413..28852
                     /gene="norV"
                     /locus_tag="ACHKV2_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO6938482.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            28849..29982
                     /gene="norW"
                     /locus_tag="ACHKV2_000542"
     CDS             28849..29982
                     /gene="norW"
                     /locus_tag="ACHKV2_000542"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO6938483.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(30110..32362)
                     /gene="hypF"
                     /locus_tag="ACHKV2_000543"
     CDS             complement(30110..32362)
                     /gene="hypF"
                     /locus_tag="ACHKV2_000543"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO6938484.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32515..33042)
                     /gene="hydN"
                     /locus_tag="ACHKV2_000544"
     CDS             complement(32515..33042)
                     /gene="hydN"
                     /locus_tag="ACHKV2_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO6938485.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33191..34201)
                     /gene="ascG"
                     /locus_tag="ACHKV2_000545"
     CDS             complement(33191..34201)
                     /gene="ascG"
                     /locus_tag="ACHKV2_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO6938486.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            34461..35918
                     /gene="ascF"
                     /locus_tag="ACHKV2_000546"
     CDS             34461..35918
                     /gene="ascF"
                     /locus_tag="ACHKV2_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO6938487.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            35927..37351
                     /gene="ascB"
                     /locus_tag="ACHKV2_000547"
     CDS             35927..37351
                     /gene="ascB"
                     /locus_tag="ACHKV2_000547"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO6938488.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37475..37945)
                     /gene="hycI"
                     /locus_tag="ACHKV2_000548"
     CDS             complement(37475..37945)
                     /gene="hycI"
                     /locus_tag="ACHKV2_000548"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO6938489.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(37938..38348)
                     /gene="hycH"
                     /locus_tag="ACHKV2_000549"
     CDS             complement(37938..38348)
                     /gene="hycH"
                     /locus_tag="ACHKV2_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO6938490.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(38345..39112)
                     /gene="hycG"
                     /locus_tag="ACHKV2_000550"
     CDS             complement(38345..39112)
                     /gene="hycG"
                     /locus_tag="ACHKV2_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO6938491.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(39112..39654)
                     /gene="hycF"
                     /locus_tag="ACHKV2_000551"
     CDS             complement(39112..39654)
                     /gene="hycF"
                     /locus_tag="ACHKV2_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO6938492.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(39664..41373)
                     /gene="hycE"
                     /locus_tag="ACHKV2_000552"
     CDS             complement(39664..41373)
                     /gene="hycE"
                     /locus_tag="ACHKV2_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO6938493.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(41391..42314)
                     /gene="hycD"
                     /locus_tag="ACHKV2_000553"
     CDS             complement(41391..42314)
                     /gene="hycD"
                     /locus_tag="ACHKV2_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO6938494.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(42317..44143)
                     /gene="hycC"
                     /locus_tag="ACHKV2_000554"
     CDS             complement(42317..44143)
                     /gene="hycC"
                     /locus_tag="ACHKV2_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO6938495.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44140..44751)
                     /gene="hycB"
                     /locus_tag="ACHKV2_000555"
     CDS             complement(44140..44751)
                     /gene="hycB"
                     /locus_tag="ACHKV2_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO6938496.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(44876..45337)
                     /gene="hycA"
                     /locus_tag="ACHKV2_000556"
     CDS             complement(44876..45337)
                     /gene="hycA"
                     /locus_tag="ACHKV2_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO6938497.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            45549..45899
                     /gene="hypA"
                     /locus_tag="ACHKV2_000557"
     CDS             45549..45899
                     /gene="hypA"
                     /locus_tag="ACHKV2_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6938498.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            45903..46775
                     /gene="hypB"
                     /locus_tag="ACHKV2_000558"
     CDS             45903..46775
                     /gene="hypB"
                     /locus_tag="ACHKV2_000558"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO6938499.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            46766..47038
                     /gene="hypC"
                     /locus_tag="ACHKV2_000559"
     CDS             46766..47038
                     /gene="hypC"
                     /locus_tag="ACHKV2_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO6938500.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47038..48159
                     /gene="hypD"
                     /locus_tag="ACHKV2_000560"
     CDS             47038..48159
                     /gene="hypD"
                     /locus_tag="ACHKV2_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO6938501.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPAVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            48156..49166
                     /gene="hypE"
                     /locus_tag="ACHKV2_000561"
     CDS             48156..49166
                     /gene="hypE"
                     /locus_tag="ACHKV2_000561"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO6938502.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49240..51318
                     /gene="flhA"
                     /locus_tag="ACHKV2_000562"
     CDS             49240..51318
                     /gene="flhA"
                     /locus_tag="ACHKV2_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO6938503.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(51355..51708)
                     /gene="ygbA"
                     /locus_tag="ACHKV2_000563"
     CDS             complement(51355..51708)
                     /gene="ygbA"
                     /locus_tag="ACHKV2_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO6938504.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(51785..51919)
                     /locus_tag="ACHKV2_000564"
     CDS             complement(51785..51919)
                     /locus_tag="ACHKV2_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938505.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            51995..54556
                     /gene="mutS"
                     /locus_tag="ACHKV2_000565"
     CDS             51995..54556
                     /gene="mutS"
                     /locus_tag="ACHKV2_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO6938506.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            54662..55318
                     /gene="pphB"
                     /locus_tag="ACHKV2_000566"
     CDS             54662..55318
                     /gene="pphB"
                     /locus_tag="ACHKV2_000566"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6938507.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(55359..55595)
                     /locus_tag="ACHKV2_000567"
     CDS             complement(55359..55595)
                     /locus_tag="ACHKV2_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO6938508.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(55606..57033)
                     /locus_tag="ACHKV2_000568"
     CDS             complement(55606..57033)
                     /locus_tag="ACHKV2_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO6938509.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(57033..57626)
                     /locus_tag="ACHKV2_000569"
     CDS             complement(57033..57626)
                     /locus_tag="ACHKV2_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO6938510.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            57773..58180
                     /locus_tag="ACHKV2_000570"
     CDS             57773..58180
                     /locus_tag="ACHKV2_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6938511.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(58300..59292)
                     /gene="rpoS"
                     /locus_tag="ACHKV2_000571"
     CDS             complement(58300..59292)
                     /gene="rpoS"
                     /locus_tag="ACHKV2_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO6938512.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(59355..60449)
                     /gene="nlpD"
                     /locus_tag="ACHKV2_000572"
     CDS             complement(59355..60449)
                     /gene="nlpD"
                     /locus_tag="ACHKV2_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO6938513.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(60634..61260)
                     /gene="pcm"
                     /locus_tag="ACHKV2_000573"
     CDS             complement(60634..61260)
                     /gene="pcm"
                     /locus_tag="ACHKV2_000573"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO6938514.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(61254..62015)
                     /gene="surE"
                     /locus_tag="ACHKV2_000574"
     CDS             complement(61254..62015)
                     /gene="surE"
                     /locus_tag="ACHKV2_000574"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO6938515.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(61996..63045)
                     /gene="truD"
                     /locus_tag="ACHKV2_000575"
     CDS             complement(61996..63045)
                     /gene="truD"
                     /locus_tag="ACHKV2_000575"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO6938516.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(63042..63521)
                     /gene="ispF"
                     /locus_tag="ACHKV2_000576"
     CDS             complement(63042..63521)
                     /gene="ispF"
                     /locus_tag="ACHKV2_000576"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO6938517.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(63521..64231)
                     /gene="ispD"
                     /locus_tag="ACHKV2_000577"
     CDS             complement(63521..64231)
                     /gene="ispD"
                     /locus_tag="ACHKV2_000577"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO6938518.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(64250..64561)
                     /gene="ftsB"
                     /locus_tag="ACHKV2_000578"
     CDS             complement(64250..64561)
                     /gene="ftsB"
                     /locus_tag="ACHKV2_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO6938519.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(64755..65078)
                     /gene="ygbE"
                     /locus_tag="ACHKV2_000579"
     CDS             complement(64755..65078)
                     /gene="ygbE"
                     /locus_tag="ACHKV2_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO6938520.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(65128..65733)
                     /gene="cysC"
                     /locus_tag="ACHKV2_000580"
     CDS             complement(65128..65733)
                     /gene="cysC"
                     /locus_tag="ACHKV2_000580"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO6938521.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(65733..67160)
                     /gene="cysN"
                     /locus_tag="ACHKV2_000581"
     CDS             complement(65733..67160)
                     /gene="cysN"
                     /locus_tag="ACHKV2_000581"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO6938522.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(67162..68070)
                     /gene="cysD"
                     /locus_tag="ACHKV2_000582"
     CDS             complement(67162..68070)
                     /gene="cysD"
                     /locus_tag="ACHKV2_000582"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO6938523.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            68322..69359
                     /gene="iap"
                     /locus_tag="ACHKV2_000583"
     CDS             68322..69359
                     /gene="iap"
                     /locus_tag="ACHKV2_000583"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO6938524.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   69441..69653
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=69502..69531
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(69749..70042)
                     /gene="cas2e"
                     /locus_tag="ACHKV2_000584"
     CDS             complement(69749..70042)
                     /gene="cas2e"
                     /locus_tag="ACHKV2_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO6938525.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(70039..70962)
                     /gene="cas1e"
                     /locus_tag="ACHKV2_000585"
     CDS             complement(70039..70962)
                     /gene="cas1e"
                     /locus_tag="ACHKV2_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO6938526.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(70959..71609)
                     /gene="cas6e"
                     /locus_tag="ACHKV2_000586"
     CDS             complement(70959..71609)
                     /gene="cas6e"
                     /locus_tag="ACHKV2_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO6938527.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(71591..72337)
                     /gene="cas5e"
                     /locus_tag="ACHKV2_000587"
     CDS             complement(71591..72337)
                     /gene="cas5e"
                     /locus_tag="ACHKV2_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO6938528.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(72348..73403)
                     /gene="cas7e"
                     /locus_tag="ACHKV2_000588"
     CDS             complement(72348..73403)
                     /gene="cas7e"
                     /locus_tag="ACHKV2_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO6938529.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(73415..73951)
                     /gene="casB"
                     /locus_tag="ACHKV2_000589"
                     /gene_synonym="cse2"
     CDS             complement(73415..73951)
                     /gene="casB"
                     /locus_tag="ACHKV2_000589"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO6938530.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(73948..75510)
                     /gene="casA"
                     /locus_tag="ACHKV2_000590"
                     /gene_synonym="cse1"
     CDS             complement(73948..75510)
                     /gene="casA"
                     /locus_tag="ACHKV2_000590"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO6938531.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(75608..78307)
                     /locus_tag="ACHKV2_000591"
     CDS             complement(75608..78307)
                     /locus_tag="ACHKV2_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO6938532.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAP
                     ELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
                     GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKE
                     DGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
     gene            complement(78499..78651)
                     /locus_tag="ACHKV2_000592"
     CDS             complement(78499..78651)
                     /locus_tag="ACHKV2_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6938533.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(78915..79649)
                     /gene="cysH"
                     /locus_tag="ACHKV2_000593"
     CDS             complement(78915..79649)
                     /gene="cysH"
                     /locus_tag="ACHKV2_000593"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6938534.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(79723..81435)
                     /gene="cysI"
                     /locus_tag="ACHKV2_000594"
     CDS             complement(79723..81435)
                     /gene="cysI"
                     /locus_tag="ACHKV2_000594"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO6938535.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(81435..83234)
                     /gene="cysJ"
                     /locus_tag="ACHKV2_000595"
     CDS             complement(81435..83234)
                     /gene="cysJ"
                     /locus_tag="ACHKV2_000595"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO6938536.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            83553..83915
                     /gene="queD"
                     /locus_tag="ACHKV2_000596"
     CDS             83553..83915
                     /gene="queD"
                     /locus_tag="ACHKV2_000596"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO6938537.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            83993..85264
                     /gene="ygcN"
                     /locus_tag="ACHKV2_000597"
     CDS             83993..85264
                     /gene="ygcN"
                     /locus_tag="ACHKV2_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6938538.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            85255..85515
                     /gene="ygcO"
                     /locus_tag="ACHKV2_000598"
     CDS             85255..85515
                     /gene="ygcO"
                     /locus_tag="ACHKV2_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6938539.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            85532..86107
                     /gene="ygcP"
                     /locus_tag="ACHKV2_000599"
     CDS             85532..86107
                     /gene="ygcP"
                     /locus_tag="ACHKV2_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO6938540.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(86255..87115)
                     /gene="ygcQ"
                     /locus_tag="ACHKV2_000600"
                     /pseudo
     CDS             complement(86255..87115)
                     /gene="ygcQ"
                     /locus_tag="ACHKV2_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(87112..87891)
                     /gene="ygcR"
                     /locus_tag="ACHKV2_000601"
     CDS             complement(87112..87891)
                     /gene="ygcR"
                     /locus_tag="ACHKV2_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO6938541.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(87869..89205)
                     /gene="ygcS"
                     /locus_tag="ACHKV2_000602"
                     /pseudo
     CDS             complement(87869..89205)
                     /gene="ygcS"
                     /locus_tag="ACHKV2_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(89299..90753)
                     /gene="ygcU"
                     /locus_tag="ACHKV2_000603"
     CDS             complement(89299..90753)
                     /gene="ygcU"
                     /locus_tag="ACHKV2_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO6938542.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(90823..91608)
                     /gene="ygcW"
                     /locus_tag="ACHKV2_000604"
     CDS             complement(90823..91608)
                     /gene="ygcW"
                     /locus_tag="ACHKV2_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6938543.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            91927..93204
                     /gene="yqcE"
                     /locus_tag="ACHKV2_000605"
     CDS             91927..93204
                     /gene="yqcE"
                     /locus_tag="ACHKV2_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO6938544.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            93231..94709
                     /gene="ygcE"
                     /locus_tag="ACHKV2_000606"
     CDS             93231..94709
                     /gene="ygcE"
                     /locus_tag="ACHKV2_000606"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6938545.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT        22437 a        25812 c        24301 g        22504 t
ORIGIN      
        1 atctttataa aatatttggt gagcatccac agcgagcgtt caaatatatc gaacaaggac
       61 tttcaaaaga acaaattctg gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc
      121 tggccattga agaaggcgag atatttgtca tcatgggatt atccggctcg ggtaaatcca
      181 caatggtacg ccttctcaat cgcctgattg aacccacccg cgggcaagta ctgattgatg
      241 gtgtggatat tgccaaaata tccgacgccg aactccgtga ggtgcgcaga aaaaagattg
      301 cgatggtctt ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt
      361 ttggtatgga attggccgga attaatgccg aagaacgccg ggaaaaagcc cttgatgcac
      421 tgcgtcaggt cgggctggaa aattatgccc acagctaccc gaatgaactc tctggcggga
      481 tgcgtcaacg tgtgggatta gcccgcgcgt tagcgattaa tccggatatt ttattaatgg
      541 atgaagcctt ctcggcgctc gatccattaa ttcgcaccga gatgcaggat gagctggtaa
      601 aattacaggc gaaacatcag cgcaccattg tctttatttc ccacgatctc gatgaagcca
      661 tgcgtattgg cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac
      721 cggatgaaat tatcaataat ccggcgaatg attatgtccg taccttcttc cgtggcgttg
      781 atattagtca ggtattcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc
      841 gtaaaacccc tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgtg
      901 aatatggcta cgttatcgaa cgcggtaata agtttgtcgg cgcagtctcc atcgattcgc
      961 ttaaaaccgc gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag
     1021 cagtcgatgc acaaacgcct cttagcgagt tgctctctca tgtcggacag gctccctgtg
     1081 cggtgcccgt ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc
     1141 tgcgcgcttt agatcgtgag ggggtaaata atggctgatc aaaataatcc gtgggatacc
     1201 acgccagcgg cggacagtgc cgcgcaatcc gcagacgcct ggggtacacc ggcgactgca
     1261 ccgactgacg gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat
     1321 tttaatattc tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa
     1381 gggatcgact gggtcgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt
     1441 gattatatcc tcaacggttt ccagcaattg ctgctgggta tgcccgcacc ggtggcgatt
     1501 atcgttttcg ctctcatcgc ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg
     1561 gtttcgctga ttgccatcgg cgcaatcggt gcctggtcgc aggcaatggt tactctggcg
     1621 ctggtgttaa ccgccctgct gttctgtatc gtcatcggtt tgccgttggg gatctggctg
     1681 gcgagaagtc cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg
     1741 ccagcgtttg tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc
     1801 gtggtggtga cgatcatctt tgctctgccg ccgattatcc gtctgaccat tctggggatt
     1861 aaccaggttc cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag
     1921 atgctgttca aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag
     1981 acgctgatgc tggccctttc tatggtggtc atcgcctcga tgattgccgt cggcgggttg
     2041 ggtcagatgg tacttcgtgg tatcggtcgt ctggatatgg ggcttgccac cgttggcggc
     2101 gtcgggattg tgatcctcgc cattatcctc gatcgtctga cgcaggccgt tgggcgcgac
     2161 tcacgcagtc gcggcaaccg tcgctggtac accactggcc ctgtcggtct gctgacccgc
     2221 ccattcatta agtaactctg cacttgcccg gtgacgccgg gcatatcacc ctgccaaaaa
     2281 aaggaataac aatgcgacat agcgtacttt ttgcgacagc gtttgccacg cttatctcta
     2341 cacaaacttt tgctgccgac ctgccgggca aaggcattac tgttaatccg gttcagagca
     2401 ccatcaccga agaaactttc cagacgctgc tggtcagccg cgcgctggag aaattaggtt
     2461 ataccgtcaa taaacccagc gaagtggatt acaacgttgg ctacacctcg cttgcttccg
     2521 gcgatgcaac cttcaccgcc gtgaactgga cgccactgca tgacaacatg tacgaagctg
     2581 ccggtggcga taagaaattt tatcgtgaag gggtatttgt taacggcgcg gcacagggtt
     2641 acctgatcga taagaaaacc gccgaccagt acaaaatcac caacatcgca caactgaaag
     2701 atccgaagat cgccaaactg ttcgatacca acggcgacgg aaaagcggat ttaaccggtt
     2761 gtaaccctgg ctggggctgc gaaggtgcga tcaaccacca gcttgccgcg tatgaactga
     2821 ccaataccgt gacgcataat caggggaact acgcggcgat gatggccgac accatcagtc
     2881 gttacaaaga gggcaaaccg gtgttttact acacctggac gccgtactgg gtgagtaatg
     2941 agctgaagcc aggcaaagat gtggtctggt tgcaggtgcc gttctctgca ctgccgggcg
     3001 ataaaaatgc cgataccaaa ctgccgaatg gcgcgaatta cggcttcccg gtcagcacca
     3061 tgcatatcgt tgccaataaa gcctgggccg agaaaaaccc ggcagcagcg aaactgtttg
     3121 ccattatgca gttgccagtg gcagatatta acgcccagaa cgccattatg catgacggca
     3181 aagcctcaga aggcgatatt cagggccacg ttgatggctg gatcaaagcc caccagcaac
     3241 agttcgatgg ctgggtgaat gaggcgttgg cagcgcagaa gtaattttta ttcgggcgga
     3301 taaggcgttt acgccgcatc cgccgttctg tacacaatgc ctgatgcgac gctggcgcgt
     3361 cttatcatgc ctacaaacca caccgcacat ttcaacaatc tatctttcat cccatattca
     3421 tcaacatccg ctattattga tttccagctt aatcatcacc tgatgaacaa aaataatgac
     3481 caaacctaat catgagctta gcccggcgct aatcgtgctg atgtctatcg ccaccggtct
     3541 ggcggtcgcc agcaactatt acgcccagcc attgctcgac accatcgcgc gtaacttttc
     3601 cctttccgcc agttcggcag gctttattgt taccgccgcg cagttgggct atgccgccgg
     3661 tctgctgttt cttgttcccc tcggtgatat gtttgaacgc cgccgcctga ttgtctcgat
     3721 gaccttactg gcagcgggcg gtatgttaat cactgccagc agtcagtcac tggcgatgat
     3781 gatcctcggt acggcattaa ccggtttatt ctcagtcgtg gcacaaattc tggttccgct
     3841 ggcagcgacg ctggcttcac cggataaacg cggcaaagtg gttggcacca ttatgagcgg
     3901 tctgctgttg gggatattgc tggcacgaac cgttgccggg ttactggcaa acctcggcgg
     3961 ctggcgcacc gtcttttggg ttgcctcggt gttaatggct ctgatggcgc tggcattatg
     4021 gcgtggtctg ccacaaatga aatcagaaac ccacctcaac tacccacagt tgttgggttc
     4081 cgttttcagc atgtttatca gcaataagat cctgcgcacc cgcgcgttgc tgggctgcct
     4141 gacctttgcc aacttcagca ttctctggac ctcaatggcc tttttgcttg ccgctccacc
     4201 ttttaactac agcgatggtg taattggtct gtttgggctt gcgggagctg ccggagcgtt
     4261 gggcgctcgt ccggcgggcg gttttgccga caagggcaaa tcgcaccaca ccacaacttt
     4321 cggtctgctt ctgctgttac tttcatggct ggcaatctgg tttggtcaca cttccgtact
     4381 ggcgttgatt atcggcattc tggtgctgga cctcaccgta cagggcgtgc atatcactaa
     4441 ccagacggta atttatcgaa tacatcctga tgcgcgtaat cgcctgaccg caggttacat
     4501 gaccagctac tttattggcg gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca
     4561 tggcggttgg gctggcgttt gtctggctgg cgcgacgatt gccctggtta acttactggt
     4621 ctggtggcga ggttttcatc gtcaggaagc cgcaaattaa gcaatcagca accgttttcg
     4681 tggctttaca cttataaggg tgttaagaag cccatcagtc tgataaggtt aagatattca
     4741 ttcagtctat ttataatatt aacaatcgtt aagcgtacac tctatggaaa gccctactcc
     4801 acagcctgct cctggttcgg cgaccttcat ggaaggatgc aaagacagtt taccgattgt
     4861 tattagttat atcccggtgg cctttgcgtt cggtctgaat gcgacccgtc tgggattctc
     4921 tcctctcgaa agcgtttttt tctcctgcat catttatgca ggcgcgagcc agttcgtcat
     4981 taccgcgatg ctggcagcag ggagtagttt gtgggttgct gcactgaccg tcatggcaat
     5041 ggatgttcgc catgttttgt atggcccgtc actgcgtagc cgtattattc agcgtctgca
     5101 aaaatcgaaa accgccctat gggcgtttgg cctgacggat gaggtttttg ccgccgccac
     5161 cgcaaaactg gtacgcaata atcgccgctg gagcgagaac tggatgatcg gcattgcctt
     5221 cagttcatgg tcatcgtggg tatttggtac ggtaataggg gcattctccg gcagcggctt
     5281 gctgcaaggt tatcccgccg ttgaagctgc attaggtttt atgcttccgg cactctttat
     5341 gagtttcctg ctcgcctctt ttcagcgcaa acaatctctt tgcgttaccg cagcgttagt
     5401 tggtgccctt gcaggcgtaa cgctattttc tattcccgtc gccattctgg caggcattgt
     5461 ctgtggctgc ctcactgcgt taatccaggc attctggcaa ggagcgcccg atgagctatg
     5521 aggttctgct gcttgggtta ctggttggcg cggcgaatta ttgcttccgc tatttgccgc
     5581 tgcgcctgcg tgtgggtaat gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg
     5641 acaccattgg catcgcctcg atatgcgctc tgctggttgt ctctaccgca ccagaagtga
     5701 tgcacgatac acgccgtttc gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt
     5761 tctataaaac acgcagcatt atcatcccaa cactgcttag tgcgctggcc tatgggctcg
     5821 cctggaaagt gatggcgatt atataaccca caagaatcat ttttctaaaa caatacattt
     5881 actttatttg tcactgtcgt tactatatcg gctgaaatta atgaggtcat acccaaatgg
     5941 atagttcgtt tacgcccatt gaacaaatgc taaaatttcg cgccagccgc cacgaagatt
     6001 ttccttatca ggagatcctt ctgactcgtc tttgcatgca tatgcaaagc aagctgctgg
     6061 agaaccgcaa taaaatgctg aaggctcagg ggattaacga gacgttgttt atggcgttga
     6121 ttacgctgga gtctcaggaa aaccacagta ttcagccttc tgaattaagt tgtgctcttg
     6181 gatcatcccg taccaacgcg acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg
     6241 aacgtcgtga aagcgataac gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc
     6301 acgagttttt gcgcgaggtt ttaccaccgc agcataactg cctgcatcaa ctctggtccg
     6361 cgctcagcac aacagaaaaa gaccagctcg agcaaatcac ccgcaaattg ctctcccgtc
     6421 tcgaccagat ggaacaagac ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc
     6481 tcaaaaatcc agatttataa aagaaaaaat gaccggccag catcgcaaca tgctggcctt
     6541 tttggcaagc aggtcggctc agccgatgag ttaagaagat cgtggagaac aatatgagcg
     6601 caaatgcgga gactcaaacc ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc
     6661 tgctcctcct tctcaccttg ctctttataa ttattgccgt agcgataggg atttattggt
     6721 ttttggtact gcgtcacttc gaagaaaccg atgacgcata cgtggcaggg aatcaaattc
     6781 aaattatgtc tcaggtgtct ggcagcgtga cgaaagtctg ggccgataac accgattttg
     6841 taaaagaagg cgacgtgctg gtcactctcg acccgacaga tgctcgccag gcgtttgaaa
     6901 aagccaaaac tgcactggct tccagcgttc gccaaaccca ccagcagatg attaacagca
     6961 agcagttgca ggcgaatatt gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact
     7021 acaaccgccg tgtgccgctg ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg
     7081 cccgcgacgc cgttaccagt gcccaggcgc aactggacgt cgcgattcaa caatacaatg
     7141 ccaatcaggc gatgattctg gggactaaac tggaagatca gcctgccgtg caacaggctg
     7201 ccaccgaagt acgtaacgcc tggctggcgc tggagcgtac tcgtattgtc agtccgatga
     7261 ccggttatgt ctcccgccgc gcggtacagc ctggggcgca aattagccca acgacgccgc
     7321 tgatggcggt cgttccagcc accaatatgt gggtggatgc caactttaaa gagacgcaga
     7381 ttgccaatat gcgtatcggt cagccggtca ctatcaccac cgatatttac ggcgatgatg
     7441 tgaaatacac cggtaaagtg gttggtctgg atatgggcac aggtagcgcg ttctcactgc
     7501 ttccggcgca aaatgcgacc ggtaactgga tcaaagtcgt tcagcgtctg cctgtgcgta
     7561 tcgaactgga ccagaaacag ctggagcaat acccgctgcg tatcggttta tccacgctgg
     7621 tgagcgtcaa taccactaac cgtgacggtc aggtactggc aaataaagta cgttccactc
     7681 cggtagcggt aagcaccgcg cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg
     7741 atatcgtaaa agctaacgct ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg
     7801 ctggaaggcg cgcaactggt cattatgacg attgcgctgt cactagcgac attcatgcag
     7861 gtgctggact ccaccattgc taacgtggcg atccccacta tcgccgggaa tcttggctca
     7921 tcgctcagcc agggaacgtg ggtaatcact tctttcgggg tggcgaatgc catctcgatc
     7981 ccgcttaccg gctggctggc aaagcgcgtc ggggaagtga aactgttcct ctggtcaacc
     8041 atcgcctttg ctattgcgtc gtgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc
     8101 ttcttccgcg tgattcaggg gattgtcgcc gggccgttga tcccgctttc gcaaagtcta
     8161 ttgctgaata attacccgcc agccaaacgc tcaatcgcgc tggcgttgtg gtcgatgacg
     8221 gtgattgtcg cgccaatttg cggcccaatc ctcggcggat atatcagcga taattaccac
     8281 tggggctgga tattcttcat caacgtgccg attggcgtgg cggtggtgtt gatgacactg
     8341 caaactctgc gcggacgtga aacccgcacc gaacggcggc ggattgatgc cgtggggctg
     8401 gcactgctgg ttattggtat cggcagcctg cagattatgc tcgaccgcgg taaagagctg
     8461 gactggtttt catcacagga aattatcatc cttaccgtgg tggcggtggt ggctatctgc
     8521 ttcctgatcg tctgggagct gaccgacgat aacccgatag tcgatctgtc gttgtttaag
     8581 tcgcgcaact tcaccatcgg ctgtttgtgt atcagcctcg cgtatatgct ctacttcggc
     8641 gcgattgttc tgctgccgca gttgttgcag gaggtctacg gttacacggc gacctgggca
     8701 ggtttggcct ctgcgccggt agggattatt ccagtgatcc tgtcgccgat tatcggccgc
     8761 ttcgcgcata aactggatat gcgccgactg gtaaccttca gctttattat gtatgccgtc
     8821 tgcttctact ggcgtgccta tacctttgaa ccaggtatgg attttggcgc gtcggcctgg
     8881 ccgcagttta tccaggggtt tgcggtggcc tgcttcttta tgccgctgac caccattacg
     8941 ctgtctggtt tgccaccgga acgactggcg gcggcatcga gcctctccaa ctttacgcgt
     9001 acgctggcgg ggtctatcgg cacgtcgata accacaacca tgtggaccaa ccgcgagtcg
     9061 atgcaccatg cgcagttgac tgagtcggta aacccgttca acccgaatgc ccaggcgatg
     9121 tacagtcaac tggaagggct tgggatgaca caacagcagg catcaggctg gattgcccag
     9181 cagatcacca atcaggggct gattatttcc gccaatgaga tcttctggat gtcggccggg
     9241 atattcctcg tcttgctggg gctggtgtgg tttgctaaac cgccctttgg cgcaggtggc
     9301 ggcggaggcg gtgcgcacta agtacaacta agccagttca tttgaactgg cttttttcaa
     9361 ttaattgtga agatagttta ctgactaaat gtgcagttcc tgcaacttct ctttcggcag
     9421 cgccagttct tcgttgctgt tgatgcgcac gtcacgttcc agaatgctac gcgcaatatc
     9481 ctgcgcttcc tgcaacgagt gcatctggta agtgccacac tggtagacgt tcagctccgg
     9541 aatctgattc tgatcctgca ctttcagcac gtcttccatt gccgctttcc agacatcagc
     9601 aacacgctgc tcatctggcg taccaatcag actcatataa aaaccggtgc ggcagcccat
     9661 tggcgagata tcgataatct ctacaccatt accgttaaga tggttacgca taaaaccagc
     9721 aaacaggtgc tccagggtat ggatccctct ttctggcatc acttctttgt tcggcacgca
     9781 gaagcgcaga tcgaacacgg tgattgcgtc gccatgcggg gtgttcattg ttttcgccac
     9841 ccgaactgca ggcgcttcca tccgggtatg atcgactgtg aagctatcta acaacggcat
     9901 ttagccacct ccggtaattt ttttaaaaat tttctgaact ctttcttccc aggcgagtct
     9961 gagtatatga aagacgcgca tttgttatca tcatccctga attcagagat gaaattttgg
    10021 ccactcatga gtggcctttt tcttttctgt caggcgtgtt tttccagcca caccgcaaac
    10081 ggttcggtat ctgcggcttc catttcctgc tgacggcgtt cagatgcctc gcgctcggct
    10141 acaaaatcct cttcgcgcag aatttccagc ggctcttcac gcagcagatt acggtacgct
    10201 tcagcaaacg ctttgcctgt tccgccaata ccagtatcaa tcatagacct taagatacgg
    10261 gcagagaaag tcagatcggg attatcgaag caggcaacca gttcatcaca cactttctga
    10321 tacgcttcgc cgccgttaat gctatccagc gtttgcgcga cgcgtttcag atcgcggaac
    10381 agatctttac ccacctgcgg taacgggaac tgtgcggttt cgcagccgat acccagcgtc
    10441 agacccggtt tgcgaccttc gaggatcacc cggttccagt taacgcgtgt acaggcaagt
    10501 tcgctactgc tcatttccgg tgcatcagcc agcgcacacc agaccataaa caggtcgagg
    10561 aatcgcacct gctgttcatc tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc
    10621 acttcaatat attcaatgcc gccacgtaac agcgcatcag aaggcgactc gccgctgcgg
    10681 gtaacgcgtt ttggacgaat cggcgcgtac agttcgtttt caatctgcag cacgttgctg
    10741 ttgatttgca gcctcttacc gtctttatca ataccaatct tcgcgtactc ttccgatggc
    10801 gttttgattg cctgtttcag gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca
    10861 agattgcttt gcgatttatt ggtatagccg agatcgctca aacgaagaga ggtcgcatac
    10921 ggcaggtaat acataccgca ctcggttttc tcaaacggca gcgacgttgg tttaccttgc
    10981 aggaatgaag agcatattgc cggagacgcg ccaaacagat aaggaatgac ccaaccgaag
    11041 cgatagtagt tgcgaataac gcggaaatag cccgcagaga ttttctcttt agcatcagcg
    11101 cccgaaatat cgccacactt cgcttgccag aatgccatag gcaaagagaa gttgtaatgc
    11161 acgccggaga tggtttgcat cagcgcgccg tagcgatttt tcagcccttc acggtacagc
    11221 gttttaaagc gtccggtgtt agaagtgccg tactgcgcca gttcgatgtc ctgaccttct
    11281 gcgatgtagc atggcatact taacggccac atccgctcat cgcccatatt gcgcgccgta
    11341 taacgatgca gatcgcgcat aaaggtcagc atatgttcaa tatcaccatc cactggtgta
    11401 atgaattcca gcaatgcttc cgcaaaatcg gtagtaatcc atttatgcgt cagtgcggaa
    11461 cctaatgctt caggatgacc tgttgttgcc agtgtgccat cagcattaac acgcaaagtt
    11521 tcgcgctcca gcccacgctg tatccccttt aacgcctgag gatgtttttc cagccaggcc
    11581 agcgcctgtg atacgtccgg gatcaaattg acctcccgcc tgtcaaaatc gttttaatta
    11641 gcataactgt aatggtgacc atatgtgcag gcctacaatt agtgccacca catcatgccc
    11701 tgaacggtcg ctgctgcaac tgcaacatag cgtaacgctt taccaaggca caaaaaaaag
    11761 attaccggcc cccaggagat gcgcatccat cccgctaaca gacacagtaa atcgccaacc
    11821 acgggcatcc agcttaataa tagcgtgact gcaccatagc gtttcagcca gccggtagct
    11881 ttctcttgcc agcgcgatgt tttacgcaat ggacagaaac gcccaaggat aacgttagtt
    11941 aaccctccaa ggctattacc cattgttgct gttaaaacta aaacccaggg atgactgatc
    12001 ccagaaagca acattgccac cagcacgact tcggagttgc cgggtaatag tgtagcgctg
    12061 agaaaactac tggcaaacaa cgagaaaagc gataacgctt cactcacagc aagcgaacat
    12121 ccacagcgtc catgcctgct gcacgggccg cctgaatacc gaaatcggca tcttcaaaga
    12181 ccacacactg cgtcggttgc acgcccatac gctgcgcgca caacaaaaat gtgtctggcg
    12241 cgggtttatg gtgtttgacg tgatcggcag cgacgacggc gtcaaaataa cggcgtaatc
    12301 ccaggtgagc cagcaatgcc tcagcgatgg cgctttcact ccccgttcct acagccattg
    12361 ggcgacgacc atgccaactt ttcaccactt caacaagagg aagcggctcg acactatcca
    12421 gcagcatact tcttactgct tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg
    12481 cctgattcag ctcaataatt gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa
    12541 tcatcgcctg aacatcgtac tgaagaccgt agtgccctaa tacttcgcgc cacgctttac
    12601 ggtgcgtagg ctccgtatcc aggattgtgc catccatatc aaaaattaaa cctgcataac
    12661 gctcgtacat ggtcttctcg caagtcgaaa aatatgacgc tactttagcg taattgcttg
    12721 attttgtcgc tgatggcgaa ggagaagaaa attcaggaaa tggataaagt agttatttag
    12781 aaatgagata tttttgaagg aaattttttg gagaagatgg tgcatccggg aggatgactc
    12841 actggcgttc gcccttcggg ccgttgctaa agcaacgtta tccttcctgg tgcctgcggt
    12901 taattccgct acctcttcga tatcttcatt gctgaagatt tcggagaata tggtgcatcc
    12961 gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta
    13021 cggatgcatc gggaaaattt attttactgc tgatattgat atcgctacta aagccatatc
    13081 aaataagaaa atccatctta accatcatca ctgatgatta cggagaatat ggtgcatccg
    13141 ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac
    13201 ggatgcatcg ggaaatttat tttactgctg atattgatac cgctactaaa gctgtatcaa
    13261 gtaagagatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
    13321 ctctatccag ctgagctacg gatgcatcgg gaaacttact ttactgcaga ttttttgata
    13381 ccgctactaa agccgtatca agtaagagat ggtgcatccg ggaggattcg aacctccgac
    13441 cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcaaat ggcggtgagg
    13501 cggggattcg aaccccggat gcagcttttg accgcatact cccttagcag gggagcgcct
    13561 tcagcctctc ggccacctca ccacacgcct cttacgagtg cttcgaagaa cttgtttatc
    13621 gctcatcgtc gctgcgtggc gcacatatta ctttctgaga cttataagtc aaacaatttt
    13681 tcccacactt ttatcgtttg cacacttcac gctcaattag tctgtaaaaa cgtcaaaaag
    13741 agtgttttat ctacagaaaa acggaggtct ggcagatagc agtaatgcaa aaaaatggag
    13801 acttaagttg aatgaacggg agtaaagcga aaagactata gagtgaagga gaaattttga
    13861 gggtgcgtct caccgataaa gatgagacgc ggaaagatta gtaactggac tgctgggatt
    13921 tttcagcctg gatacgctgg tagatctctt cacggtgaac agaaacttcc ttcggggcat
    13981 ttacgccaat acgtacctgg ttgcccttta cccctaaaac tgtcacggtg acctcatccc
    14041 caatcatgag ggtctcacca actcgacgag tcagaatcag cattctttgc tccttgaaag
    14101 attaaaagag tcgggtctct ctgtatcccg gcattatcca tcatataacg ccaaaaagta
    14161 agcaatgaca aacacattac atgtaagcag tcatggcatt acattctgtt aaacctaagt
    14221 ttagccgata tacacaactt caacctgact ttatcgttgt cgatagcgtt aatgcgaatg
    14281 ccgtgaagcg agtccacggc attgcctgac gcttatatta ttgcaatttc gcgctgaccc
    14341 agcctttcac actggctaac gctgcaggta aggccgcagc atccgtacca ccggcttgcg
    14401 ccatgtcagg acgtccacca cccttgccgc ccacctgctg agcgaccata ccaatcagtt
    14461 cccctgcttt cacacgatct gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac
    14521 cttcggctac cgttgccagc acgataattg tcgaccccag ctgatttttt aaatcgtcaa
    14581 ccatggtacg caacattttc ggctcaacac cgctaagctc gctaaccaac agcttaacac
    14641 cattaacatc aattgcctta ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt
    14701 taagctgttg tagttctttt tctaactgac gcgtacgttc cagtactgag cgcactttat
    14761 ccgccagatt attgctatcg cctttcagca gatgcgcgac ttcgcttaag cgatcgctgt
    14821 ctgcatgaac ggtggtgata gcgccttctc cggttaccgc ttcgatacga cgaacgcctg
    14881 cagcagtacc cgattcagag atgatgcgga acagaccaat atcaccagtg cggctggcgt
    14941 gagtaccgcc acacagctcg gtggagaaat cgcccatgct cagcacgcgt acacgctcat
    15001 catacttctc gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg agatccatga
    15061 tgttggtttc gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac
    15121 gaatctcttc cggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt
    15181 taaccagtga acctttctgc gatacatgag tccccagaac ctggcgcagc gcagcgtgca
    15241 tcaggtgcgt tgcggagtga ttcaaacgaa tacgggcgcg acgagcctca tcaacatcag
    15301 cctgcacagc gtcgcccact ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg
    15361 cctggccgta tttctgcgta tcttccacca cgaaggagaa gttagcgcct ttcagttcgc
    15421 ctttatcacc aacctgaccg ccggattccg catagaatgg cgtttgatcc agcacgacca
    15481 cagcttcctg gcctgcattg atggcatcaa ccgctttacc atcaacaaac agcgcggtca
    15541 ctttgccgtt cagttccaga tggtcatagc ctttaaattc agatgcactg tcaacacgga
    15601 tcattgcgtt gtaatcggca ccaaagccgc tggcttcgcg cgcccgacga cgctgctctt
    15661 ccattgctgc ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag
    15721 ccgtcaggtc aaccgggaag ccataggtgt cgtacagacg gaaagcagtt tcaccatcca
    15781 gcgtatcacc agaaagtttt gccagctctt catccagcaa cgccagaccg cgctccagag
    15841 tacgagcaaa ctgctcttct tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt
    15901 tcaggtcttc acccgcagag cccataacgt cgatcagcgg accaaccagt ttgtagaaga
    15961 aggtttcttt cgcgccgagc atattaccgt gacgcactgc gcgacgaatg atacgacgca
    16021 gtacataacc acggttttca ttggacggca ttacgccatc cgcgatcagg aacgcacaag
    16081 aacgaatgtg gtcagcgatt acgcgcagcg atttattgct cagatcggtt gcgccagtga
    16141 ctttcgctac cgcctggatc aacgtgcgga acaggtcgat gtcatagtta gagttaacgt
    16201 gttgcagcac cgcagcaata cgctccagac ccataccggt atctacagac ggcttcggca
    16261 gcggttccat cgtgccatcg gcctggcggt tgaactgcat gaagacgatg ttccagatct
    16321 caatgtagcg gtcgccgtct tcttccggac ttcccggagg gccaccccaa atgtggtcgc
    16381 cgtgatcgta gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc atctgccaga
    16441 agttgtcaga tgcgtatggc gcacctttgt tatcgccgat gcgaataata cgttcgcgcg
    16501 ggatccctac ttctttttcc cagatttcgt aggcttcgtc gtcgctttca tagacggtaa
    16561 cccacagacg ctctttcggc agggcaaacc atttttcgct ggtcagcagt tcccatgcaa
    16621 actgaatggc atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga
    16681 aggtatggtg acgcgcggtg taaccgacgt tttccaggtc gttgtgttta ccacccgcac
    16741 gcacgcagcg ttgggaagtg gtagcgcggg aataattacg cttgtcgagc ccaaggaaca
    16801 catccttgaa ctggttcatc ccggcgttgg taaacaacaa agttgggtcg ttatggggta
    16861 ccagggagct gctggcaact acctgatgtc ccttactatg gaaaaagtcg agaaacgcct
    16921 gacggatctc agcggtgctc ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg
    16981 ccaccagctc cagcgtgtct taaccgccgg gctggtaact gaaaagtggg aataagataa
    17041 gttttcttga ctgggaagta aaataccgta tgcgttcagt cggcaaaatt tcgccaaata
    17101 tcctggatat cttccatcag atagccacga tagagcagaa aacgctggat cttaactttt
    17161 tctgaaaaga cagttggcaa aggttcacca tattttcgcg tcgcctgatc gcgcgccagt
    17221 gcgcaccagt cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct
    17281 ttctgattca gttcctggcg aatacgcgca ggtccataac ctttgcggct acggctggcg
    17341 ataaagcgcg caacaaatcg gctgtcatcg agatagccat gttcatggca ccaggcaata
    17401 acgcgctcgt aatcttctgc cgtagcatca atctcttctg ggccattttt gcccataatc
    17461 ggtgccgcga gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt
    17521 accgcacgat ccaacaggcg agcatatgcc gggcgacggg acgttgattc tgtcatggca
    17581 tatccttaca acttaaaaag caaaagggcc gcagatgcga cccttgtgta tcaaacaaga
    17641 cgattaaaaa tcttcgttag tttctgctac gccttcgcta tcatctacag agaaatccgg
    17701 cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt
    17761 ttccgggtta tctttcagcc aggcagttgc attcgcttta ccctgaccga tcttctcacc
    17821 tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac
    17881 cagttcgccg tagaagttga taccttcgcc gtagaggatc tggaattcag cctgtttaaa
    17941 cggcgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc
    18001 gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag
    18061 cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat
    18121 ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat
    18181 cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat
    18241 ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga
    18301 acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag
    18361 caggttgtcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc
    18421 atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcaatcacct gcaacgtcag
    18481 cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc
    18541 acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat
    18601 ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc
    18661 cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat
    18721 gccgggtaat accggatagt caatatgttc tgttgaagca attatactgt atgctcatac
    18781 agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct
    18841 gacgacgcac cgcatcacgg tcgccgctga agcattcccg cagggtaatg ccttcaccgc
    18901 gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg
    18961 gcccggcgat accactaata gacacggcat aatcagcacg agccgctttc agtgcaccta
    19021 tcgccatttc caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc
    19081 gtacgccgat catttgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc
    19141 aggcggaact gccggcaata tcggtaatca ctttcgctac ccaaccgccg gtacaagact
    19201 cagcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcgcttaact
    19261 gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga
    19321 tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa
    19381 aagctgatta gccagaggga agctcacgcc cccctcttgc aaatagttac tgcacgcgcg
    19441 ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggta ctgtggttat
    19501 aacgggtaat ggtgtagaag ttcggcagac cgtaccagta ctggtaacct gttccaacat
    19561 ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg
    19621 ccgcggcaag ctgcgaaatg ctgtacctgg ttttgaagcc atttggcaag cctggagcct
    19681 gaccgtttgc cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaataat
    19741 tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga
    19801 aatctaccgc atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg
    19861 caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc agcaggaagg
    19921 tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca
    19981 gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa
    20041 tcccgacgat aatttccggc ggtacgccat acacctgcca cgcgcgattc aacgcatctt
    20101 catactgatt ccagaaaacc acaccgttct gcacattgtc cggcgtaata aattttttgc
    20161 gataacgcag ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct
    20221 ggttatccat cagccgcagt accgaatcca gacgtttcgc ctgggagaga atttcctgca
    20281 actgctgacg atcgaaaccg tgtttgttca ccattctgtc gatgaactgc tgggcattcg
    20341 ggttattagc gaaatcgccg cccatctgca tcacattgtg ctgcggctca agcaggaagc
    20401 cgccagacgg cgttccggtg gtcgtttcag tctcagtagg ttttggcttg ctgctacagg
    20461 cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg acgcttgaac atgaggggtc
    20521 catttaacag attcaaccag gggcaagtat ggtaaagcat cacgccccgc acaaggaagc
    20581 ggtagtcact gcccgatacg gactttacat aactcaactc attctcctcg ctatcctttt
    20641 actcaaactt tcaaattaaa atatttatct ttcattttgc gatcaaaata acactttaaa
    20701 tctttcaatc tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag
    20761 agaacaatga tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag
    20821 ggcggagagg tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg
    20881 gttatcatga acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa
    20941 cgttgcgccg gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc
    21001 ttttgcaatc cgatgacctt aagcctgggt cgctttatgc cggaaaagta caaacccagc
    21061 tactacgcgg cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac
    21121 cctggcgaac tgtttgttat cttggcattg ccagcggtct gacaacgctg aacctgccac
    21181 ttggcccact ggcggtgagc tatctgctgg ttggtctggt caccaacttc ttccgcggct
    21241 gggtgaccga tctgaccacc gctatttttg agaaaaagat gggcattcaa cttgaacaaa
    21301 aagttcatct ggcaggagca acatcatgac gcatattcgg atcgaaaaag gaacgggggg
    21361 ctggggcggc ccgcttgagc tggaaaccac gccgggcaaa aaaatcgtct atatcacccc
    21421 gccggtaccc ggcctgcgat tgtcgacaaa ctggcacagc ttactggctg gcaggctatt
    21481 gacggattta aagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt
    21541 ggcggcacat tacgctgcgg catttatccg aaacggcgta ttcccaccat taatatccac
    21601 tcgacgggca agtccggtcc actggcgcag tacattgtgg aagatattta tgtctctggc
    21661 gtaaaagaag aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc
    21721 cgtgactatg acaccagcaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg
    21781 ggaatgggta tgggttctgc cgttgccgtg ttgtttcaat ctggtcgtga caccatcgac
    21841 actgtattaa aaaccattct tccgtttatg gcattcgtct cggcgctcat tggcatcatt
    21901 atggcttctg gccttggtga ctggattgcc cacggccttg ctccgctggc gagccatcca
    21961 ctgggtctgg tcatgctggc gctcatctgc tccttcccgc tgctttcacc tttcctcggc
    22021 ccaggcgcag ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc
    22081 aatattccgc cgcatctggc tttacctgca ctgtttgcca tcaacgcgca ggcggcctgc
    22141 gacttcattc cggtcggttt gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt
    22201 gtcccttctg tactggtgag ccgcttttta accggcgcgc cgactgtact gatcgcctgg
    22261 tttgtctccg gttttatcta tcaatagagg ctgaaacatg accgttattt atcagaccac
    22321 catcacccgt atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt
    22381 tcgtgaaggc gcgccagcgg acctcgaaga gtattgcttc attcattgcc acggcgagtt
    22441 gaaaggtgca ctccatcccg gtttgcaatt tacgctcggg cagcatcgct atccggtgac
    22501 cgctgttggc agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt
    22561 cgatggttta aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtacccga
    22621 cgatatcgcg ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca
    22681 ggttgccgtt gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc
    22741 tgccgagggg tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc
    22801 acaagaaatt aacgccgaat atggtgaagg aacagcgtat ggttttggtg ctgacgccac
    22861 tagcgagcaa agcgttctgg cgctctctcg tggggtagat gaaatctttg ttcgtgtgga
    22921 tttactggtc tacagcgccg gaattgcaaa agcagccttt atcagcgact tccagctcgg
    22981 cgattttgac cgttcgctac aggtgaatct ggtgggttat ttcctgtgtg cgcgtgaatt
    23041 ttcgcgtttg atgatccgcg atgggattca gggacgcatt attcagatca actcgaaatc
    23101 cggcaaagtg ggcagcaaac acaattctgg ttatagcgcc gcgaaatttg gtggcgtagg
    23161 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
    23221 gctcggtaac ctgctgaaat cgccgatgtt ccagtcgctg ttgccacaat atgccaccaa
    23281 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    23341 cggctgcgat tatcaggatg tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc
    23401 gtactgtacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc
    23461 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    23521 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcactgt
    23581 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    23641 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    23701 ctttaaaccg cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgccgacac
    23761 cttgtttatg aaaggactga ccgtcttcgc ccgaccgcaa aaaattcccg caattaccgg
    23821 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    23881 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    23941 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    24001 tcaagccgcc attctggagt atctgcaaaa gcagggaaaa tgctcggttg aagaattggc
    24061 gcaatatttt gacaccacag gcacaactat tcgcaaagat ctggtcattc tggaacatgc
    24121 cggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    24181 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    24241 tggttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    24301 ggtgcctctg ctctcgcgct ttaataacat cacggtgatg accaacagcc tgcatatcgt
    24361 caatgcgtta tctgaactgg ataatgaaca aactatcctg atgccaggcg gaacgtttcg
    24421 taaaaaatcg gcctcatttc acgggcagct ggcagagaat gccttcgagc atttcacctt
    24481 cgacaaatta ttcatgggta ccgacggcat cgatctcaat gcgggcgtaa ccaccttcaa
    24541 cgaggtctat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    24601 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    24661 taagctgatt accgacgcag atatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    24721 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    24781 agacgttaat gctggaattg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    24841 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    24901 gcaaatcggg ccacattggt aagaaaatcg ccgcaacgct tgccagtacc ggcacaccgg
    24961 ctttttttgt ccatccggca gaagcgctgc atggcgatct ggggatgatc gaaagccgcg
    25021 atgtgatgct gtttatctct tactccggtg gggcgaagga actggatctg attattccgc
    25081 gtctggaaga caaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    25141 gcctggcggc gaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    25201 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    25261 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccct gctggagcac
    25321 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    25381 aggtggcgtt aaccgccagc gtgatggatg ctatgctgga actcagccgc accggtctgg
    25441 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    25501 tacgtcgctg gctggttggc ggcggcgcac tcaccacgcc agtcaatgaa gcgatgacgg
    25561 tcggcggcac cacgttgcaa tcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    25621 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaataa
    25681 acctgcagga tttctatcag gccgggatta tttaatcctt caatcccaga cgtttcgcca
    25741 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttgtgat
    25801 gattttgtgc cagtgcctga cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    25861 ggttttgctt aacaacgggc accgccgcca cttctggcgg cggcaacgtc acctcaggaa
    25921 aagcaaaatg ttgcgcctca agaatcactt catcgccgct gcgggtggct ctcgccagca
    25981 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt tcccggaaaa ctgtagtgtt
    26041 gcagtaaatt tcgcgccccg gcacttaata ccacgcggga gagcccctgc cgcaaacgac
    26101 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    26161 gcaccgaaag tggaaacaca ctcaggcgat gaaacaaatc ggcgcggaat cgccctgcca
    26221 gcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    26281 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    26341 gcaatgccaa cgacaactcg ccgatctcat cgagaaacag cgtgccgtta tccgccattt
    26401 caaacttccc actgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    26461 actcactttc cgccacactt tccggcagtg cggcacagtt gagatagacc agcggattca
    26521 ccgcccgtgg cgaggcctca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    26581 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    26641 gcgtcatgcc tggcgacaga ccgatcatct gcgtctgttt caccgcttca aatggagcgg
    26701 catcgcccgg cagcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    26761 ctcccgccgc cagcgcggca atcaggcgta actcttcgtc gctgaaaaca tcgaactgat
    26821 cgggctgcat cccgtcgagc gtcaatgcgc cgatcaggtt ttgcccggca aacagcggca
    26881 gaccaacgca ggcgtgaacc ttcagactct cctgccctgg aatcaaaccg tcatagggat
    26941 cgggcaattc gctgtctgcg ggaaagcgca ccacatcacc cgcgcgggca atcgcttcca
    27001 gccgtggatg accttccagc gcaaagcgtc taccgagtac atccttcgcc agcccgtcga
    27061 tggcaagcgg aataaactgc cgcgagtcgt aacgtagcaa cgcagacgca tcgcactcca
    27121 gcacctgacg cagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    27181 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    27241 caatttgact atagatattg tcatatcgac cattggattg atagtcattt tgactactca
    27301 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgcaat
    27361 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    27421 tgtggtgaaa aataacattc attgggttgg tcaacgtgac tgggaagtac gagattttca
    27481 cggcacggaa tataaaacgc tgcgcggcag cagctacaac agctacctca tccgcgaaga
    27541 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    27601 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    27661 ccacgccggg gcgctgaccg aactgatggc acaaattccc gatacgccga tctactgtac
    27721 agccaacgct atcgactcga taaatggtca tcaccatcat ccggagtgga attttaatgt
    27781 ggtgaaaact ggcgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    27841 accaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    27901 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    27961 tcagacggag ctttttgagc agtgccagcg ttactacgcc aatatcctta cgccgttcag
    28021 ccgtctggta acgccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    28081 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    28141 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    28201 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    28261 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    28321 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    28381 cgccgggctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    28441 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    28501 gggtttcgaa atgtcgctta gcctgaaagc gaaatggcga ccagaccagg acgctctgga
    28561 gttatgccgt gaacacggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    28621 gagcacggtg aatacggtag ttaaagaaga aacctctgcc accacgacgg ctgacctcgg
    28681 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    28741 gcaggacgtt gcgccaggaa cgccgtggag tgaagtcccg gataacttcc tgtgcccgga
    28801 atgctccctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    28861 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    28921 gacgccagta ttccattaac cctgattgcc gccgacagta tggatgagta caacaaacct
    28981 gacctcagcc atgttatcag tcaggggcaa cgtgccgacg accttacccg ccagacggcg
    29041 ggtgaatttg ccgagcagtt taatctgctc ctgtttccgc aaacctgggt gacggatatc
    29101 gatgccgaag cccgtgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    29161 ctggcaaccg gtgccagcgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    29221 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    29281 ttgattgttg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    29341 aaaatggtta cgctaatcga caacgctgcc agtattctgg cgtcgttaat gccaccggaa
    29401 gtaagcagtc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    29461 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgc
    29521 agcatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac cgccctggca
    29581 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    29641 aatgccgata tttacgcgct gggcgattgc gcggaaatta acggtcaggt attgccgttc
    29701 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    29761 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    29821 gcaggcgaaa cccagcgtca ggatttacgc tggcagatta ataccgaacg ccagggaatg
    29881 gtggcgcgcg gtgttgacga tgcagaccaa cttcgcgcct ttgtggtcag tgaggatcgg
    29941 atgaaagagg catttggatt gttgaaaacg ttgccggtgt aggtgggcta ctgtgcctaa
    30001 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccgg
    30061 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    30121 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    30181 catcacccgc cggtaaactc tgtggaaaca gcaatgtgaa atcagcgaga taatgcgcca
    30241 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    30301 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    30361 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagtgg
    30421 cgagatccag ttgattgtcc acccgcggca tcgtcaccgg atgcgtcact ccgtcgcatg
    30481 aggctgctag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    30541 cacagcccag tgccgccgcc acagcatcga acaaacgccc acacgatgac gccagcggcg
    30601 cgttaattcc acgctcaatg gcccgcgcca gtacgctcca gttttgctgt tgcacacttg
    30661 ccgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggttac
    30721 gccacggctg cttcgccgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    30781 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccattct
    30841 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc agcggccact
    30901 gatgctctgc cagacacgcc gctgcgtggg catgatgatg cagcaccgtt tgcgtcggca
    30961 gattcatttc gctcgcccac tggcaggaga catagcccgg atgtgcgtca tgcacaacgt
    31021 attgcggagt gaaattgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcgtct
    31081 ggatgccatc gtcacttaaa tcgcccagat gctggctcaa caccacttgt tcaccgcgca
    31141 ccaggcagaa ggtatttttc agatccgcgc cgagacacag tacaggcgga acatttttaa
    31201 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    31261 cgctttcgcg caccaccgaa tcatccatcc gctgcacaat gtcgcggttg tgtattaaga
    31321 atccgtcggc aatgccctgc aaatcctcca gcgcctgttc gttgctgata gccggtggtt
    31381 taccgctcag gttgccagag gtcatcacca gcgggcattg tagttcctgt aacagcaaat
    31441 gctgaagcgg attcgcaggc aacattaccc cgacttcgtt aaggccaggg gcgatatcat
    31501 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    31561 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgact gccagcggtt
    31621 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    31681 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    31741 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    31801 gatacgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    31861 cgcggtactc tttatcacag gctggacaca gcggaaacgc cgccatcacg gtaaacgggc
    31921 gatcgtaagg catggcgcga ataatggtga agcgcgggcc gcagtgggta cagttgataa
    31981 atggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    32041 tggcggcatc gggaacaatt tgcgtattca tggcgccgcc cgcgctctgg cggatggtga
    32101 actcagtggg cagttgtgac cagataaacg gttcacgctc aacgctatca atacgcgcca
    32161 gcggcgggca gtgctgatgc aattgaacaa gaaacgtttc cgggtcttcc agcagccgga
    32221 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    32281 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc tcgaatacgc agttggacac
    32341 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    32401 agccggacac gacagcgtcg catccggcag tcacaggtcg gcgataccgc ccgctccgta
    32461 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    32521 catcagcgcc gtacggcggc gtttttctgc gctcagttgt tcaagtttat tgcgatcgac
    32581 acaaattagc gcatgggtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    32641 gcacaggtcg catttattgg cttcggcttt gtcagcccgt acattcagcc ctgcgccgct
    32701 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    32761 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    32821 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    32881 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    32941 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacggca
    33001 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    33061 cagttaatgc gccgatacat tcacaaatca tgccagtttt taatttgctg ttatttaagg
    33121 aaattaattt ctgtaatgca ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg
    33181 atgacaatgt ttatcgcgaa agagcaatga gtgagtcgcg gcggatcagt tttccgctga
    33241 aggttttcgg cggtgagaaa tccccgccat cgagcataaa aatcagccgt ccaataattt
    33301 cctgaatcat ctcagtcacc ggaattttta cgctggagag cgccggaacg atataggggg
    33361 caattgcgat atcatcgaat ccgataactg acacctgctc tggcaccgct acgccgcgct
    33421 tgtgtaacgc tttcatcgca cctatcgcca tatcgtcgtt actggcaact aacgcgctaa
    33481 atttagcccc acgttcgagc aacgtttcta ccccttcggc cccgctggcg ggcgtccatt
    33541 taccgttagc gataagtttt tcattgagcg caataccatg ctgcgccagc gcgtctttat
    33601 atccggcaag acgttcaacg ctggtggggg aatccatcga gccggtaaga aaagcaatct
    33661 cctgatgccc ggcgtttatc aactctgcca cggcgttaaa gctggtctgt ttatgatcgc
    33721 accagacgct atggctgcta tttttgcgca ggcggcgatt aagcaccatt atcggctgac
    33781 tgtgcgcgtc aatgatgtca tcgatctcat ccacacttaa aaaacgcggg taaatcataa
    33841 tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg gcgctcttct tcggcgctgt
    33901 gtttaccatc tgccaatagc aactgccgcc ctttctcttc cgccattcgc gcggcatgaa
    33961 acagtaattc actaaaataa atgccgtggt aaagcgtgtt agtcactacc agccccagcg
    34021 tctgggtact tttcgccgac agattgcgcg ccagcaagtt tggacggtaa ccgctctctt
    34081 ctaccgcctg aaacacgcga tctttagtct cctggctgac gtagccatta cctgaaagca
    34141 cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca
    34201 tcattttcat ccctttacac gcaatcaacg cagtgtactg caccgtttac cgattgtcct
    34261 tgcgcaatcg gcgggaaaaa tattcaggtg accggtttca caaatataaa aaatgaacaa
    34321 ttaactctct tgcttatttg gttacaacta ttcatgattt tgtgaaaccg gtttcttaat
    34381 tccgtttcag catcggcatt tttccgtcac gtcgactgat aacaactaca tcttccctac
    34441 tgataacagg ataaaatccg atggccaaaa attatgcggc gctggcacgc tcggtgatag
    34501 cggcactggg cggcgttgat aacatctcgg cggtcacgca ctgtatgacg cgtttgcgct
    34561 ttgttatcaa agatgatgcg cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc
    34621 tcggcgtggt acgtagtgac aaccagtgtc aggtgattat tggcaatacc gtttcacaag
    34681 cctttcagga agtcgtcagc ctgctgccgg gagatatgca gcccgccctg cccgtgggta
    34741 aacccaaact cacgctgcgt cgcattggtg cggggatcct cgatgcgctg atcggcacca
    34801 tgtcaccgct gatcccggcg attatcggcg gatcgatggt caaactgctg gcgatgatcc
    34861 tcgagatgag cggcgtgctg acaaaaggat cgccgacctt aaccattctg aatgtgattg
    34921 gtgacggtgc tttcttcttc ctgccgctga tggtcgcggc ttcggccgcc atcaaattta
    34981 aaaccaatat gtcgctggcg attgccatcg cgggtgtgct ggtacatccg agttttattg
    35041 aactgatggc gaaagcggcc cagggtgaac atgtcgaatt tgccctgatt ccagtcaccg
    35101 cggtgaaata cacctacaca gtgatcccgg cgctggtgat gacctggtgc ctgtcatata
    35161 tcgaacgctg ggtggatcgc attacaccgg cggtgaccaa aaacttcctc aagccaatgc
    35221 tgattgtgtt gattgccgca ccgctggcaa tcctgctgat tggcccgatt ggtatctgga
    35281 tcggtagcgc catttcggcg ctggtttaca ccattcatgg ttatctgggc tggctttcag
    35341 tcgccattat gggcgcgctg tggcctctgc tggtaatgac cgggatgcac cgcgtcttta
    35401 cgctaaccat cattcagacc attgccgaaa ccggcaaaga agggatggtc atgccgtcag
    35461 agatcggcgc taacctgtcg ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa
    35521 acccggaact gcgccagact gcgctggctg cggcggcatc agccattatg gcggggattt
    35581 ccgaaccggc gttatacggc gtggcgatcc gcctgaaacg tccgcttatc gccagtctta
    35641 tcagcggttt tatttgcggc gcggttgccg gtatggcggg gcttgccagc cactcaatgg
    35701 cagcgccggg gctatttacc agcgtgcagt tcttcgatcc ggcgaatcca atgagcatcg
    35761 tctgggtgtt cgcggtcatg gcgctggcgg tggtgctgtc gtttatcctc acactgttgc
    35821 tcggctttga ggatattcct gttgaggaag cggctgccga ggcgcgaaag catcagagcg
    35881 cacaaccgac cgtcgccaaa gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc
    35941 agaaggtttt ttatggggcg gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga
    36001 aggtggcaaa ggactgacaa cggtcgatat gatcccacac ggcgagcatc gaatggcggt
    36061 gaaactgggg ctggaaaaac gttttcagtt gcgcgatgac gagttttatc ccagccatga
    36121 ggcgacgggt ttttatcatc gttataaaga agatatcgcc ctgatggcag agatgggatt
    36181 caaagtgttc cgtacctcaa ttgcctggag ccgccccttc ccgcagggtg atgaactgac
    36241 gcccaaccag cagggcattg ctttttaccg ttctgtcttt gaagagtgta aaaagtacgg
    36301 tatcgaaccg ctggtcacat tgtgccactt cgatgtgccg atgcagctgg tcaccgaata
    36361 tggctcctgg cgtaaccgca agctggtgga gtttttcagc cgctacgcca ggacctgctt
    36421 tgaagcattt gatggtctgg tgaaatactg gctaaccttc aatgaaatca acattatgtt
    36481 gcatagcccg ttctccggcg cggggctggt gtttgaagaa ggtgaaaatc aggatcaggt
    36541 gaaatatcag gccgcgcatc accagctggt tgccagtgcg ctagccacca aaatcgccca
    36601 tgaggttaac ccgcaaaatc aggtgggctg tatgctggcg ggcggtaact tctaccctta
    36661 cagttgcaag ccggaagatg tctgggcggc gctggagaaa gatcgggaaa acctgttttt
    36721 tatcgatgtg caggcgcggg gcgcgtatcc ggcttactct gctcgcgtat tccgcgaaaa
    36781 aggggtaacc atcgacaaag caccgggcga tgatgagatc ctgaaaaaca ccgtcgattt
    36841 tgtctctttc agctattacg cctcgcgctg cgcctcggcg gagatgaaca ccaacaatag
    36901 cagtgcggcg aacgtagtga aatcgctgcg taacccgtat ctacaggtga gcgactgggg
    36961 ctggggaatt gatccactcg gtctgcgtat caccatgaac atgatgtacg accgctatca
    37021 gaagccgctg tttctggtgg aaaacggcct cggcgcaaaa gatgaacttg ctgccaatgg
    37081 cgagattaac gatgactatc gcatcagcta tttacgcgaa catatccgcg caatgggcga
    37141 agcgattgca gatggcattc cgctgatggg ctacaccaca tggggctgta ttgatttagt
    37201 ttccgcctct acgggtgaaa tgagcaaacg ctacggtttt gtctttgttg accgtgacga
    37261 cgcaggcaac ggcacgctga cgcgcacacg taagaaatcg ttctggtggt ataaaaaagt
    37321 gattgccagt aatggggaag atttagagta ggtaacctac tgtcgcacta accaggaagg
    37381 cccgataagc gttgcgcatc gggcaatctg gggtttgcta tcagttctga ttccctcatc
    37441 aagaacatcc ctgtcctgat tacttattga aaaactactc ttcttccacc gctaactgcg
    37501 cgaagccgcc gttcccttcc cagccttcca gtcgttgata aacggtttct accgcatctt
    37561 taatcggctg ggtcatcggg tagtaaaagc cgacgatatc cggctggatg ccgaggaaaa
    37621 tcacttcgcc aatatcttct ttcaactgat cgataaggta gttcaacggc atgttatggg
    37681 tagtcatcat aaacatctcg gcgatatcat ccgggtcgat gatgcggatt tcgccggggt
    37741 ttagccccat atccgtggcg tcgacaatca gcagccgtgt cgggcgcagt tcgcggatag
    37801 cgacaatgtc gttttccggt gcgctaccgc cgtcaatcac cacccagtta cctttcggcg
    37861 cggcggcgca cttttccgcc agcagcggac ctgcgccatc atcacccatc atgctattgc
    37921 caacacagag taaaacgtca gtcacgcagt ctcctcacca tcaaatagat ggcgttttcc
    37981 tgatgaatat catgcagcat acttaacagc attgtactcc attcctgctg ctgcgaagtt
    38041 tgcgctgccc gggctttatc gaatgcattg gccagcatcg gcacatggtt aatgtcgatg
    38101 acgatctcac catatttcgg cacaccttcc attttgcggc gggcttcgct gcccgcctcc
    38161 agagtggcaa tccatgccag atattcatcc cacgggcagg tgagcgccgc ttccaggcaa
    38221 tcgataaccc caaggtggtg accaatcgcc aggctgtaat agaccacctg ctgcgcctcg
    38281 gcgggcgtgg catcgttctc atcaataaat ttacggctca gttgactgaa caccaccttt
    38341 tcactcatcg gatacgcgcc tcttcaacaa catgattcag atggctgaca atctcgttca
    38401 gacgcggatc gttttccgct tccagccagc gtgcaacctg ttcttcgccc tgccctaact
    38461 gtgtaaggta atcatcggca atctgacgac cgtaacgata acccgccagt cgacgtgctt
    38521 cgcgatccac tttcacacgc agcggctgca ccatatcgcc atgcaggatc tccgccggtt
    38581 gttcatcctg ctcacccggc ccacgggcgt gaattttctg ctccagcagg ccgagcgcca
    38641 ttgcaaagcc gtacagcgtg gcggctggcg ttggcgggca gccagggata taaacatcca
    38701 ccgggacgat tttatccgta ccgccccaca cgcagtagag atcgtggaag atcccgccgc
    38761 tgttaccgca ggcaccgtag gagatacaaa ttttcgggtc cggcgcggac tgccacgcac
    38821 gcagcgcagg ggatcgcatt gcacgggtga ccgcgccggt aaacagtaaa atatccgcat
    38881 gacgcggtga aggaacgact ttaatgccga agcgttctgc atcgaacagc ggcgacaaag
    38941 tggcgaaaat ttcgatttcg cagccgttgc agccgccgca gtccacgcgg taaacgtagg
    39001 cagaacgttt gatttttttc agtaacgacg ccttcatgct ggcgatggat tcatccaccg
    39061 tcatcgggac cggaatgccg ttggcgtcac gggggcctaa taaattgctc atcagatggc
    39121 ctctttcata tggcgagtca gttcaatacg gtcggacggc accaggcatt tctggcgctt
    39181 acattccggg caagtctcaa agctttcgcg gtggttttcc gcgcggctgt cgccattgtg
    39241 cttaagcagc gcaatggcgt agtcgatctc tttctggacg gcgaaaggac gattgcagac
    39301 gcggcagttg cacagcgcga agcgggactg ttgcaggaag tcttctttct tccacaccgc
    39361 cagttcgtac tcttgcgaca gtttgattgc cgccgtcggg cagacttctt cgcagcgtcc
    39421 gcagaagatg cagcgcccga gattgaactc ccacgccagc tcaccggtgg cgaggtcagt
    39481 ttcaaccgtt aaggcgtttg acgggcaggc attgacgcag gccgcgcagc cgatgcactg
    39541 ctgcgggttc tgctctggct taccacggaa gtttttatca accgcaatcg gttccagcgg
    39601 ataagacgag gtcgccgtgc cggttttgat gacttttttg ataaaggtaa acatggcgat
    39661 tccttatttc agcggcgagt ttttacgctc aatgctgtaa cgctcgagtt ctttgtacgg
    39721 caccactttg ctctttttct tacgcacatc gaccacggtc atgcggtcgg tacaggagta
    39781 gcaagggtcc agactaccga taatcagcgg cgcatcagaa acggtgttgc cgcgcagcat
    39841 gtagcgcagg gtcggccagt tcgcgtaggt cgcggcacgg cagcgccagc ggtacagctt
    39901 ctggttgtcg ccggtcatgc tccagtggat atcatcgccg cgcggcgctt cggcaaagcc
    39961 cagcgcaaaa cggtgcggaa tgtaggtaaa gccttccacc atcagcgggc cgcccggcag
    40021 gttatccaga ccgtagtcga tcatgttcag cgcggtatag acttcgttaa tacgcacttt
    40081 cagacgcgaa ataacgtcgc agccctgctc gctgtggact tccattggca gcaggccata
    40141 accaacaaac gggtgatcgg cgcgggtatc gcgggcgtga ccgctggcac ggaccatcgg
    40201 gccgacgtta ctgaagtcgc gagcgatttc cgggtccaga cgaccaatgc cgacagtgcg
    40261 ctgttccatg ttcggcgtac tcagcagcac atccaccagc tcctgcactt cacgacgcat
    40321 ctgttgcgcc agttgacggg tctggatcat gtcatctttc agcagatcgc gacgaatccc
    40381 gccgatcagg ttcaggccgt aggttttacg cgcaccggta aggatctctg ccattttcat
    40441 ggaggtttca cgcacgcgga agaactgcat aaagccggag tcaaagccgg taaagtgaca
    40501 ggcgaggccg aggttgagca gatgcgagtg cagacgttct acctccagca gaatggcgcg
    40561 gatcatctgc gcacgttctg gcacctgaat acccatcgcg ttttccaccg acgtggtgta
    40621 ggcggtgctg tgagcaaaac cgcagatccc gcacacacgg tcagagagga aggtcacttc
    40681 gttataaccc atgcgggttt ctgccagttt ttccatgccg cgatggacat agaacaggcg
    40741 gtagtcggcg tcgataatgt tttcgccatc gacgaacaga cggaagtggc ccggttcgtc
    40801 agaagtgacg tgcagcggac caatcggcac gacgttgttt ttcttgtcgc ccagttcgtt
    40861 gatgaactcg taggtttcag catcggtggt cggtgccgga cgctgacgat aatccatgct
    40921 gtctttacgc agcggataaa gttcatccgg ccagtcatcc ggcagcacca gacgacgttc
    40981 atccggcaaa ccaaccggaa tcaaaccgta catatcacgt acttcacgct cgccccacac
    41041 cgcagccgga acgcgcggcg tcacggaagg atactccggt ttgttggcat caacttcgac
    41101 gcgaaccgtt acccagcact tagtcccctt ctccatcgac agcacgtagt aaacggcgta
    41161 atgaccattc agtttgcgtt cgtcgttacc aaaaagcacc gacagccagc ccccctgctt
    41221 gtagtagaga aactccacca cttccggcag atagttcacc tttacggtga cagtcagctg
    41281 atctttggtc tgccaggcgt ggtccagcac gacgcccgga aatgcctcat tcagcgcggc
    41341 gagataatgt tgacctaatt tttcttcaga catgctcaaa ctctctttaa tcacgccgcc
    41401 agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag cccaggtaat gcgcggagta
    41461 atatcaagac gcagacgggc catgctgttt tcgaacagcg cgataaccag gacgccgacc
    41521 accagtttga cgatggcaat caccaacgcc agcagcagcc caccgacggt gaaggtttcc
    41581 atttgtcccc acggaataaa caccccgacg aacatctgca gcaccaccaa ctgtttcagg
    41641 ctgatacccc atttcatgac gccaaagccg ctgccgctgt actcagagag cggaccttcc
    41701 tgcaactcct gctcggcttc cgccaggtcg aacggcagtt tgcccatttc gataaaagtg
    41761 gcgaacgcac aggcacaaag cgccagcacc agcgggatgc tctggctcag cggccagtga
    41821 taaacggtgt cggtgatgtt gctgatgttg gtggaactgg caacctgtgc ggcaacccac
    41881 agaccaagca gcagcatcgg ttcgaccagc acgccaagca tcgcttcacg gctcgcgccg
    41941 atagcggtaa acggactacc ggtatccaga ccagaaatgg caaagaagaa acgcgcgatg
    42001 gcaaagagat acagtaaggt gatcaaatca cccagttgcg gcagcggaga accgacggtc
    42061 accaccggca gcgcagtagc gatagtcagc atgacgccca ccatcacata cggcgtcagg
    42121 cggaacaccc agccggaggc atccgggcca acgctctggc gacccagcag tttgataatg
    42181 tcgcgatact cctgcaacac gcccggcccg cgacggttat gcaagcgggc gcgcgccacg
    42241 cgggtaatac cggagagcag cggcgcaacg gcaaatagca ccagcgcctg aattaacgga
    42301 tataaaacac tcattctcag gctcctcgtg aaacaataat caccaccagc accgccagtt
    42361 caaccagcgc catccggcgg aacagcaacg cactgccttc gcactgccag cccggcacca
    42421 gcgacaccgg attcagccat ttgcgcagtt tcagcaccgg cgcaaacgcc tgtttcaccg
    42481 gcatggcaaa accgtgggcg gtaattacca ttgatttttc atggtcgtag ccgcacaccc
    42541 aggccgcgcc gcgggaacgc gatggcaaac gatcgccttt gcaaatcgcc ataatgatga
    42601 atggcagcag cgggcaggca atcagcagca acgtgatcat cggttgagaa acggtggtgt
    42661 tagcaggttc cagcggcaga ggtacagcag cagagagcat cggcagcagc cacggcgcag
    42721 caacaccgcc aattacgcag caaatcgcca gtgccactac gcttacgctc atcaggagcg
    42781 gtgcacaggt ggcgttttcg gcttctttgg tacgcggcgc gccgaggaac gtgacgccat
    42841 agactttcgc catacacatc accgccagcg caccggtaat tgccagaccc acagcgagca
    42901 gcggccccag cagacgggca acaaacgcgc cactattgct cagtttgaag aaggattgat
    42961 agataaccca ttccccggca aaaccgttca gcggcggcag tgcagccatt gccatcagcc
    43021 cgactaacat agcgatggag ataaccggca ttttcttgcc aataccaccg agtttttcga
    43081 tatcgcgatg accggtacgg aaccagacgc tccccgcccc gaggaacagt acgcttttga
    43141 acaggctatg gttaagcaga tggtacagac caccgaccag gccaagagca atcagcgccg
    43201 gttgttcgag cgcgataccc gttacgccag cgcccagccc cagcaggata atgccgatat
    43261 tttccagggt gtggtaagcc agtagacgct ggatattatg ttccatcaac gcatacagac
    43321 cgccgacaaa cgcggtgatc ataccgagca ccagcagcgc gatcccccac cacagcggtg
    43381 cattaccgcc cagcagtgac agggttaaaa tgcccagcag gccaattttc atgactaccg
    43441 tagaaaacaa cgcggcagct ggcgcagagg cgttcgcatg tgcctgcggc acccagccgt
    43501 gcagcggaat aatcccggcc agcaggccaa agccaatcac actgagcagc cagatatcgg
    43561 aaccgagcgg cagctgttgc atacgcatat ccagcaggcg cagatccagc gtgccgtaac
    43621 gctgccacag cagccagcaa gcaatcgcca gcagcagagt gccaagacgc cccagcgcaa
    43681 accacagttt gccctctttg ctgttgctgg tgaggaacac cgcacacaag gccatgactt
    43741 cggccattac cacgaacatg ccgaggttgc tggcaatgac ggcgcagacg gcggcagcca
    43801 tcaacatatt gatctgcaag ccgttgcact tcacctgcgc gtggcgatgc cagtcaatgt
    43861 tgtagaggct gacaaacaga ccgcacagac cgagcgtaat cagccaaatc gcgttaagcg
    43921 gagagatttg cacatcgtag cttaccagcg acagcgcacc gctcacgcca accgcgccag
    43981 tcagtacagt gaagcccgcg gctgccgtat acagactacc aaccgcgccg ccaattccgg
    44041 ctatccagcc gcttagcgcc ttttgaaaag aaaagagaaa tgccagaaca gcggcggcga
    44101 caaaccacgc cacgccgcta ttgatcaggg aaattgcgct catttagcct ctccactttg
    44161 agcctgctga aacaaggtga gatcgccaaa gtccgtgtta aaggtcagct cacgcttacg
    44221 tttgctgacg cgggcgatat cggtgttatc caccagatgc agggctttag tcgggcacat
    44281 ccgcacgcag gccggacctt gttcatcaaa gctacaaagg tcacatttga cggcgatcgc
    44341 gcgaataccc ggcacccagt caagcagcgt actgatacgc gccggagcag gcggtgccgg
    44401 tggcgctttc ggggtattgg catttgcagg aatatccagc ggacggctgc cggaaaattc
    44461 aattgcgcca aacgggcagg cgatgccgca cagcttgcag cttacgcaca ggctttcatt
    44521 caactgcacg gccccatcaa cgcgggtgat ggcgttaacc gggcagacca ccgcgcaggg
    44581 tgcatcttca cagtggtgac agagctgcgg cgcagattct ttttcattta gcatcactct
    44641 caggcgcggc attgattgca ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca
    44701 agtgtggcaa ccgatacaga gcgtggagtc agcaattaca aaacgattca ccaggcattc
    44761 ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt cgacactcat
    44821 cgacacgccc atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg
    44881 ctgccgggct ttgtcccttt accagttggc ttaaattcac cggcacattg ttttcaatag
    44941 cggtgtataa gctgtcgtaa acgccagcga ttttttcgag atagtgttcc agaacctgtt
    45001 cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg
    45061 caatccgctg tttgtcttcg ccatcttttg tttcgacgta atactcgatg gtgtggctgt
    45121 taaagccatc cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt
    45181 ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac gtgatcccct
    45241 gaaccagcga attgcagtag atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga
    45301 tgtaatcggc tttctcgctt atttcccaaa tagtcattgt caggttaccc gtttaacaga
    45361 gatgccagct ttaagcattt tttgtgccaa cttttaattt attattatta aagcgatttt
    45421 taagattttg gtgaatttcg tcgccgtgtc gacgtgtcat ttcgacatca tcgacattat
    45481 tcactgcagg gataatcaac actggcacaa ttattgcttg tagctggcaa tagttaatgg
    45541 gaggcgatat gcacgaaata accctctgcc aacgggcact ggaattgatc gaacagcagg
    45601 ccgcaaaaca cggcgcaaaa cgcgtaactg gggtctggct caaaattggc gcattttctt
    45661 gtgtcgaaac cagctctctt gccttttgtt ttgatctggt ttgccgcggc agcgtggcgg
    45721 aaggttgtaa actgcacctc gaagaacaag aggccgaatg ctggtgtgaa acatgccaac
    45781 agtatgtgac gctactgacc cagcgcgtcc gccgctgtcc acagtgtcat ggtgacatgc
    45841 tacagattgt ggcagacgac ggtttacaga ttcggcggat agaaatagac caggagtgag
    45901 cgatgtgtac aacatgcggt tgcggtgaag gcaacctgta tatcgagggt gatgaacata
    45961 accctcattc cgcgtttcgt agcgcgccat ttgccccggc ggcacgcccg aagatgaaaa
    46021 tcaccggcat taaagcgcct gaatttaccc ccagccagac tgaagaaggc gacctgcatt
    46081 acggtcatgg cgaagcgggc actcacgcac cgggcatgag ccagcgtcgg atgctggaag
    46141 tcgaaattga cgtgctggac aaaaataacc gtctggctga acgcaaccgc gcgcgctttg
    46201 ctgcctgcaa gcaactggtg ctcaacctgg tttccagccc aggttccggt aaaaccaccc
    46261 tgctgacgga aaccttaatg cgcctgaaag acagcgttcc gtgcgcggtt attgaaggtg
    46321 accagcaaac cgtgaacgat gccgcacgca ttcgcgctac cggcacacca gcgattcagg
    46381 tgaacaccgg taaaggctgc catcttgacg cacagatgat tgccgacgcc gcaccgcgtc
    46441 tgccactgga cgataacggt attctgttta tcgaaaacgt tggcaacctc gtatgcccgg
    46501 ccagcttcga tctcggtgaa aaacacaaag tggcggtgct ttccgttacc gaaggtgaag
    46561 acaaaccgct gaaatatccg catatgtttg ccgccgcctc gctgatgctg ctcaacaaag
    46621 ttgacctgtt gccgtatctc aactttgacg ttgagaagtg catcgcctgc gcccgcgaag
    46681 tcaatccaga aattgaaatc atccttattt ccgccaccag cggcgaaggg atggaccagt
    46741 ggctgaactg gctggagaca cagcgatgtg cataggcgtt cccggccaga tccgcaccat
    46801 tgacggtaac caggcgaaag tcgacgtctg cggcattcag cgcgatgtcg atttaacgtt
    46861 agtcggcagc tgcgatgaaa acggtcagcc gcgcgtgggc cagtgggtac tggtacacgt
    46921 tggctttgcc atgagcgtaa ttaatgaagc cgaagcacgc gacactctcg acgccttgca
    46981 aaacatgttt gacgttgagc cggatgtcgg cgcgctgttg tatggcgagg aaaaataatg
    47041 cgttttgttg atgaatatcg cgcgccggaa caggtgatgc agttaattga gcatctgcgc
    47101 gaacgtgctt cacatctctc ttacaccgcc gaacgccctc tgcggattat ggaagtgtgc
    47161 ggtggtcata cccacgccat ttttaaattc ggcctcgacc agttactgcc tgaaaacgtt
    47221 gagtttatcc acggtccggg ttgcccggtg tgcgtactgc cgatgggcag aatcgacacc
    47281 tgcgtggaga ttgccagcca tccggcagtc atcttctgta cctttggcga cgccatgcgc
    47341 gtgccgggga aacagggatc gctgttgcag gcaaaagcac gcggtgccga tgtgcgcatc
    47401 gtctattcgc cgatggatgc gttgaaactg gcgcaggaga atccaacccg caaagtggtg
    47461 ttcttcggct taggttttga aaccaccatg ccgaccaccg ccatcactct gcaacaggcg
    47521 aaagcgcgtg atgtgcagaa tttttacttc ttctgccagc atattacgct tatcccgacg
    47581 ctgcgcagtt tgctggaaca gccggataac ggtatcgacg cgttcctcgc gccgggccac
    47641 gttagtatgg ttatcggcac tgatgcctat aattttatcg ccagcgattt tcagcgtccg
    47701 ctggtggtgg ctggtttcga accccttgat ctactgcaag gcgtggtcat gctggtggag
    47761 cagaaaatag cggcccacag caaggtagag aatcagtatc gtcgggtggt accggatgcc
    47821 ggtaacctgc tggcgcaaca ggcgattgcc gatgtgttct gtgtcaacgg cgacagcgaa
    47881 tggcgcggct taggcgtgat tgaatcttct ggcgtgcacc tgacgccgga ttatcaacga
    47941 ttcgatgccg aagcacattt ccgcccggca ccgcagcagg tctgcgatga cccgcgcgcg
    48001 cgttgtggcg aagtcttgac gggcaaatgt aagccgcatc aatgcccgct gtttggtaac
    48061 acctgtaatc ctcaaaccgc gtttggtgcg ctgatggttt cctccgaagg agcgtgcgcc
    48121 gcgtggtatc agtatcgtca gcaggagagt gaagcgtgaa taatatccaa ctcgcccacg
    48181 gtagcggcgg ccaggcgatg cagcaattaa tcaacagcct gtttatggaa gcctttgcca
    48241 acccgtggct ggcagagcag gaagatcagg cacgtcttga tctggcgcag ctggtagcgg
    48301 aaggcgaccg tctggcgttc tccaccgaca gttacgttat tgacccgctg ttcttccctg
    48361 gcggtaatat cggcaagctg gcgatttgcg gcaccgcgaa tgacgttgcg gtcagtggcg
    48421 ctattccgcg ctatctctcc tgtggcttta tcctcgaaga aggattgccg atggagacac
    48481 tgaaagccgt agtgaccagc atggcagaaa ccgcccgcac ggcaggcatt gccatcgtta
    48541 ctggcgatac taaagtggtg cagcgcggcg cggcagataa actgtttatc aacaccgcgg
    48601 gcatgggcgc aattccgacg aatattcact ggggcgcaca gacgctaacc gcaggcgata
    48661 tattgctggt tagcggtaca ctcggcgacc acggggcgac tatccttaac ctgcgtgagc
    48721 agctggggct ggatggcgaa ctggtcagcg actgcgcggt gctgacgccg cttattcaga
    48781 cgctgcgtga cattcccggc gtgaaagcgc tgcgtgatgc cacccgtggt ggtgtaaacg
    48841 cggtggttca tgagttcgcg gcagcctgcg gttgcggtat tgaaatttct gaatcagcgc
    48901 tgccggttaa acctgccgtg cgcggcgttt gcgaattgct gggactggac gccctgaact
    48961 ttgccaacga aggcaaactg gtgatcgccg ttgaacgcaa cgcggcagag caagtgctgg
    49021 cagcgttaca ttcccatcca ctggggaaag acgcggcgct gattggtgaa gtggtggaac
    49081 gtaaaggtgt tcgtcttgcc ggtctgtatg gcgtgaaacg aaccctcgat ttaccacacg
    49141 ccgaaccgct tccgcgtata tgctaataaa attctaaatc tcctatagtt agtcaatgac
    49201 cttttgcacc gctttgcggt gctttcctgg aagaacaaaa tgtcatatac accgatgagt
    49261 gatctcggac agcaagggtt gttcgacatc actcggacac tattgcagca gcccgatctg
    49321 gcctcgctgt gtgaggctct ttcgcaactg gtaaagcgtt ctgcgctcgc cgacaacgcg
    49381 gctattgtgt tgtggcaagc gcagactcaa cgtgcgtctt attacgcatc gcgtgaaaaa
    49441 gacaccccca ttaaatatga agacgaaact gttctggcac acggtccggt acgcagcatt
    49501 ttgtcgcgcc ctgatacgtt gcattgcagt tacgaagaat tttgtgaaac ctggccgcag
    49561 ctggccgcag gtgggctata cccaaaattt ggtcactatt gcctgatgcc actggcggcg
    49621 gaagggcata tttttggtgg ctgtgaattt attcgttatg acgatcgccc ctggagcgaa
    49681 aaagagttca atcgtctgca aacatttacg cagatcgttt ctgtcgtcac cgaacaaatc
    49741 cagagtcgcg tcgttaacaa tgtcgactat gagttgttat gccgggaacg cgataacttc
    49801 cgcatcctgg tcgccatcac caacgcggtg ctttcccgcc tggatatgga cgaactggtc
    49861 agcgaagtcg ccaaagaaat ccattactat ttcgatattg acgatatcag tatcgtctta
    49921 cgcagccacc gtaaaaacaa actcaacatc tactccactc actatcttga taaacagcat
    49981 cccgcccacg aacagagcga agtcgatgaa gccggaaccc tcaccgaacg cgtgttcaaa
    50041 agtaaagaga tgctgctgat taatctccac gagcgggatg atttagcccc ctatgaacgc
    50101 atgttgttcg atacctgggg caaccagatt caaaccttgt gcctgttacc gctgatgtct
    50161 ggcgacacca tgctgggcgt gctgaaactg gcgcaatgtg aagagaaagt gtttaccact
    50221 accaatctga atttactgcg ccagattgcc gaacgtgtgg caatcgctgt cgataacgcc
    50281 ctcgcctatc aggaaatcca tcgtctgaaa gaacggctgg ttgatgaaaa cctcgccctg
    50341 accgagcagc tcaacaatgt tgatagtgaa tttggcgaga ttattggccg cagcgaagcc
    50401 atgtacagcg tgcttaaaca agttgaaatg gtggcgcaaa gtgacagtac cgtgctgatc
    50461 ctcggtgaaa ctggcacggg taaagagctg attgcccgtg ctatccataa tctcagtggg
    50521 cgtaataatc gccgcatggt caaaatgaac tgcgcggcga tgcctgccgg attgctggag
    50581 agcgatctgt ttggtcatga gcgtggggct tttaccggtg ccagcgccca gcgtatcggt
    50641 cgttttgaac tggcggataa aagctccctg ttcctcgacg aagtgggcga tatgccactg
    50701 gagttacagc cgaagttgct gcgtgtattg caggaacagg agtttgaacg cctcggcagc
    50761 aacaaaatca ttcagacgga cgtgcgtcta atcgccgcga ctaaccgcga tctgaaaaaa
    50821 atggtcgccg accgtgagtt ccgtagcgat ctctattacc gcctgaacgt attcccgatt
    50881 cacctgccgc cactacgcga gcgtccggaa gatattccgc tgctggcgaa agcctttacc
    50941 ttcaaaattg cccgtcgtct ggggcgcaat atcgacagca ttcctgccga gacgttgcgc
    51001 accttgagca atatggagtg gccgggtaac gtacgcgaac tggaaaacgt cattgagcgc
    51061 gcggtattgc taacacgcgg caacgtgctg cagctgtcat tgccagatat tgctttaccg
    51121 gagcctgaaa cgccgcctgc cgcaacggtt gtcgctcagg agggcgaaga tgaatatcag
    51181 ttgattgttc gcgtgctgaa agaaactaac ggcgtggttg ccgggcctaa aggcgctgcg
    51241 caacgtctgg ggctgaaacg cacgaccctg ctgtcacgga tgaagcgact gggaattgat
    51301 aaatcggcat tgatttaact gcaaattgcc ggacagatct gcctgtccgg catactattc
    51361 acgaggtttt ttcggacgat atttttccgg cagttctggc accggacact tgtcatcgat
    51421 gagatgacgc acggttaaga tcggatgacg ccacagcatt ctcggcccgg cccaacgcat
    51481 aatctgtttc atctcttcac gctttgcagg ctggtaacag tgcaccggac actgcttaca
    51541 ggctggtttc tcttcgccga acacacattt atccagccgc ttttgcgcgt aaacaaacaa
    51601 cgcctcgtaa tgctccggct ccgctgacgc ctgcgggcat ttcgcttgat aaagatcgat
    51661 cattttttta atcgtcagtt tttcacgaga gatacgcttg ccggacatac tgcctccacc
    51721 tcattaagat gcatttatat tacaacttaa tcttaaaggg cactatgact ctaaagaaga
    51781 agggttagcc aaccgataca attttgcgta cttgcttcat aagcatcacg caaaagctgc
    51841 aaaacagcat ctttcccgga accagcatca agaactcgcc gttcgcttct tcccctgaaa
    51901 tgattaactc cggtatcatg tgcgccttat gtgattacaa cgaaaataaa aaccatcaca
    51961 ccccatttaa tatcagggaa ccggacataa ccccatgagt gcaatagaaa atttcgacgc
    52021 ccatacgccc atgatgcagc agtatctcaa gctgaaagcc cagcatcccg agatcctgct
    52081 gttttaccgg atgggtgatt tttatgaact gttttatgac gacgcaaaac gcgcgtcgca
    52141 actgctggat atttcactga ccaaacgcgg tgcttcggcg ggagagccga tcccgatggc
    52201 ggggattccc taccatgcgg tggaaaacta cctcgccaaa ctggtgaatc agggcgagtc
    52261 cgttgccatc tgcgaacaaa ttggcgatcc ggcgaccagc aaaggtccgg ttgagcgcaa
    52321 agttgtgcgt atcgttacgc caggcaccat cagcgatgaa gccctgttgc aggagcgtca
    52381 ggacaacctg ctggcggcta tctggcagga cagcaaaggt ttcggctacg cgacgctgga
    52441 tatcagttcc ggtcgttttc gcctgagcga accggctgac cgggaaacga tggcggcaga
    52501 actgcaacgc actaatcctg cggaactgct gtatgcagaa gattttgctg aaatgtcgtt
    52561 aattgaaggc cgtcgcggcc tgcgccgtcg cccgctgtgg gagtttgaaa tcgacaccgc
    52621 gcgccagcag ttgaatctgc aatttgggac ccgcgatctg gtcggttttg gcgtcgagaa
    52681 cgcgccgcgc ggactttgtg ctgccggttg tctgttgcag tatgcgaaag atacccaacg
    52741 tacgactctg ccgcatattc gttccatcac catggaacgt gagcaggaca gcatcattat
    52801 ggatgccgcg acgcgtcgta atctggaaat cacccagaac ctggcgggtg gtgcggaaaa
    52861 tacgctggct tctgtgctcg actgcaccgt cacgccgatg ggcagccgta tgctgaaacg
    52921 ctggctgcat atgccagtgc gcgatacccg cgtgttgctt gagcgccagc aaactattgg
    52981 cgcattgcag gatttcaccg ccgagttgca gccggtacta cgtcaggtcg gcgacctgga
    53041 acgtattctg gcgcgtctgg cgttgcgtac cgctcgccca cgcgatctgg cccgtatgcg
    53101 tcacgctttc cagcaactgc cggagctgcg tgcgcagtta gaaactgttg atagtgcacc
    53161 agtacaggcg ctacgtgaga agatgggcga gtttgccgag ctgcgcgatc tgctggagcg
    53221 agcaatcatc gacacaccgc cggtgctggt acgcgacggt ggtgttatcg catcaggcta
    53281 taacgaagag ctggatgagt ggcgcgcgct ggctgacggc gcgaccgatt atctggagcg
    53341 tctggaagtc cgcgagcgtg aacgtaccgg cctggacacg ctaaaagttg gctttaatgc
    53401 ggtgcacggc tactacattc aaatcagccg tgggcaaagc catctggcac ctatcaacta
    53461 tatgcgtcgc cagacgctga aaaacgccga gcgctacatc attccagagc taaaagagta
    53521 cgaagataaa gtcctcactt caaaaggcaa agcactggct ctggaaaaac agctttatga
    53581 agagctgttc gacctgctgt tgccgcatct ggaagcgttg caacagagcg cgagcgcgct
    53641 ggcggaactc gacgtgctgg tgaacctggc ggaacgggcc tataccctga actacacctg
    53701 cccgaccttc attgataaac cgggcattcg cattaccgaa ggccgccatc cggtggttga
    53761 acaggtgctg aacgagccat ttatcgccaa cccgctgaat ctgtcaccgc agcgccggat
    53821 gttgattatt accggtccga acatgggcgg taaaagtacc tatatgcgcc agaccgcact
    53881 gattgcgctg atggcctata tcggcagcta cgtaccggcg caaaaagtcg agattggccc
    53941 gattgaccgt atctttaccc gcgtaggggc agcggatgat ctggcttccg ggcgttcaac
    54001 ctttatggtg gagatgaccg aaaccgctaa tattctgcat aacgccaccg agtacagtct
    54061 ggtgctgatg gatgagattg ggcgcggaac gtccacttac gatggtctgt cgctggcgtg
    54121 ggcgtgcgcg gaaaatctgg cgaataagat taaggcgttg acgctgtttg ccacccacta
    54181 tttcgagctg acccagttac cggagaaaat ggaaggcgtc gccaacgtgc atctcgatgc
    54241 actggagcac ggcgacacca ttgcctttat gcatagcgtg caggatggcg cggcgagcaa
    54301 aagctacggc ctggcggttg cagctctggc cggcgtgcca aaagaggtta ttaagcgcgc
    54361 acggcaaaaa ctgcgtgagc tggaaagcat ttcgccgaac gccgccgcta cgcaagtgga
    54421 tggtacgcaa atgtctttgc tgtccgtacc ggaagaaacc tcgcctgcag tcgaggcact
    54481 ggaaaacctc gatccggatt cactcacccc gcgtcaggcg ctggaatgga tttatcgcct
    54541 gaagagtctg gtgtaataat aattcccgat agtcttttgc tatcgggaat attaacgata
    54601 actgacgaat caaataaaaa taccctgtat aataggaaag cttattttac agggtaaaac
    54661 catgccatct acacgctatc aaaaaatcaa tacccatcac tatcgccata tatgggtcgt
    54721 tggtgatatt catggtgaat atcagttatt acaatcccgc ttacatcaac tctctttttt
    54781 ccccgaaacc gacttactta tttctgtcgg cgataatatt gatcgtgggc cggagagtct
    54841 taacgtcctg cgcctgctaa accagccctg gtttatctcc gttaaaggca accacgaagc
    54901 aatggcgctg gatgcgttcg cgaccggcga tggcaatatg tggcttgcca gcggtggtga
    54961 ctggtttttc gatttaaatg attcagagca aaaagaagct acagatctgt tgctgaaatt
    55021 ccatcacctt ccacatatta ttgaaatcac taacgacaac ataaaatatg tcatcgcaca
    55081 tgcagattat ccggggagtg aatatctctt tggtaaagaa atagcggaga gcgaattact
    55141 ctggcctgtt gatcgtgtgc agaaatcgct taatggcgag ttacaacaaa taaacggcgc
    55201 tgattatttt atatttggac atatgatgtt tgataacatt cagacgttcg ctaaccagat
    55261 ttatattgat accggatcgc cgaaaagcgg gcggctgtca ttttataaaa taaagtaatg
    55321 aggccgggta aagcaaagcc actacccggc actttttatt agcgcttacc ttccgccagc
    55381 agcggcggga tgctcggcac cattggcgcg tcatcaatat ctttctgcgt catgcggaac
    55441 gcttcgggat aatgttcgcg gctggtacgg cgcagcggtt cggtatcgcg ccaggtataa
    55501 aggcaatgct ggcactgata taccgtccag acatctttca ccggcgattt cgccatcact
    55561 tcaatctgtt catcggcaca acgtggacaa atcatcttgt tctccttatt tacgtgcggc
    55621 cagcatagcg gtcagttttt cagcccaggc tttggtttcc ggcaaatcca ccaccggctg
    55681 gctgtagtga ccacggttgt ccggggcgac aggcgtggtg gcgtcgataa tcagcttgtc
    55741 ggtgatcccc gccgggcttg agccagggtc gagttccagc acggacatat tcggcaactg
    55801 caccaaatcc cctgccggat ttactttcga ggagagcgcc cacatcacct gcggcaggtt
    55861 gaacgggtca acgtcttcat cgaccataat caccatcttt acgtagccca gaccgtgcgg
    55921 cgtggtcatc gcacgcaggc ccaccgcgcg ggcaaagccg ccgtagcgtt ttttggtgga
    55981 gataatcgcc agcaggccgt gggtgtacat ggcgtttacc gcctgcactt ccgggaactc
    56041 ggctttcagt tgttgataca gcggcacaca ggtggctggc cccatcaggt agtcgatttc
    56101 ggtccacggc atgccgaggt acagcgattc gaaaatcggc ctggtgcggt aagagacttt
    56161 atcgatacgc accacggtca tgttacgccc gccggagtag tgcccggtaa actcaccgaa
    56221 cggcccttcg atttcgcgtt tgcggctttc gataacccct tcgaggatca cttctgaacc
    56281 ccacggcaca tcaaaaccag tcaatggcgc ggtggcgatc gggtacgggc tttcgcgcag
    56341 cgcgcctgcc atttcatact cagactgatc gtatttcagc ggcgtggcgc ccataagggt
    56401 gatgatcgga tcgttaccca gggtgatggc aatcggcaga tcttcaccgc gctcttccgc
    56461 tttatgcaga tgcagggcga tatcgtgcat cggcaccggt tgcaggccga gcttacgctt
    56521 gcccttcact tccatgcggt agataccgac gttctgcttg ccgaagttat ccgggtcgag
    56581 cggatcgcgg gaaaccacgc acgctttgtc gagatagaaa ccgccatcac cgtcgtttaa
    56641 acgaaacagc ggcaggatat cgaacagatt aatctcgtca ccatcgacgg tgttctgcgc
    56701 ccaggctgga ttggcgcggc gctccggggc gatcgggaag ttatcccagc ggcggataaa
    56761 ctcatcaatc tgttttttaa ccggggtgtt tggcggcagg cccaaggaaa tcgcgtggtt
    56821 ctgccaggaa ccgatggtat tcatcgccac gcgggcatcg gtaaaaccgc gaatattatc
    56881 aaaccacagc gccggtgcac catcgccgat acgcccggtg gcgttggcag cggcagccag
    56941 atccggttcc gcgttcacct cttcgctgat tttcagtaac tggccgtggt catcaagcgc
    57001 ctgtaaaaag ctgcgtaaat catcaaatgc cattattcat tctcctgaga aaaattccgg
    57061 gcctgcggca atccttgcca gcgcctggcg tagggatgtt caaggccaaa ttgatccagc
    57121 acgcgggcta ccacgtggtg gacaatgtca tctaccgttt cgggatggtt ataaaaggca
    57181 ggcatcggcg gcaccatcgc cacgcccatg cgtgaaagtg cgagcatatt ttcgagatgg
    57241 atggtgctaa gcggcatttc acgcggcacc agcaccagtt tgcggccttc tttaagcacg
    57301 acgtccgccg cgcgccctac caggccatca gcgtaaccag cgcggatacc ggcgagcgtt
    57361 ttcatactgc acggaataac gatcatgccg tctgtacgaa aggaacctga ggagatggtc
    57421 gccgcctgat ccgccgggtt atggctgaag tcagcgaggg cagcaacatc gcgggcgctg
    57481 taaggcgttt ccagttcaat ggtggttttc gcccacttcg acatcaccag atgagtctcg
    57541 acattcggca tctcccgcag cgcttgcagt aatgccacac caagaggcgc accggtagcc
    57601 cctgtcatcc cgacgatcag tttcattgct tactctctta attgttcgta tacgaacatt
    57661 attattaaaa tacgcctctg ttctcattgc ggcaagcact gaaaagtaac aacatcccct
    57721 tcgtcactga aaaaccgcta tgatagcggc agatagtttg cccggagttt acatgacgtt
    57781 acgaaataaa gcgttccatc agttacgaca gctttttcag cagcacacgg ctcgctggca
    57841 gcacgagtta cctgacctga ccaaaccaca gtatgcggtg atgcgcgcca ttgctgataa
    57901 gcctggtatc gaacaggtgg cactgataga agcagcagtc agcaccaaag caacgttggc
    57961 agaaatgttg gcaagaatgg agaatcgtgg cttagtcaga cgtgaacatg atgccgctga
    58021 caagcggcgt cgctttgtct ggctgactgc cgaaggagaa aagatacttg ctgcggcgat
    58081 accgattggt gacagcgtgg atgaggaatt tttggggcgt ttgagtgcag aagagcagga
    58141 gctgtttgtg cagctggtgc gcaagatgat gaacacatag gatgcaaatt gccgggtagg
    58201 acgctgacgt gtcttatcca ggcgacaaaa acacaagctt cccaaacaaa agaaaaaggc
    58261 cagcctcgct tgagactggc ctttctgaca gatgcttact tactcgcgga acagcgcttc
    58321 gatattcagc ccctgcgttt gcaggatttc gcgcaaacgg cgcaggcctt caacctgaat
    58381 ctggcgaaca cgttcacggg tgaggccaat ttcacgacct acatcttcca gtgttgccgc
    58441 ttcgtacccc agcaaaccga atcgacgtgc cagtacttca cgctgtttgg cgttcagctc
    58501 gaacagccat ttgacgatgc tctgcttcat atcgtcatct tgcgtggtat cttccggacc
    58561 gttctctttt tcatcggcca ggatgtccag caacgctttt tcggaatcac cacccagcgg
    58621 ggtgtctacc gaggtaatgc gctcgttaag acgaagcata cggctgacgt catcaactgg
    58681 cttatccagt tgctctgcga tctcttccgc acttggttca tggtccagct tatgggacaa
    58741 ctcacgtgct gttcgcaggt aaacgttcag ctcctttacg atgtgaatcg gcaaacgaat
    58801 agtacgggtt tggttcataa tcgcccgttc aatcgtctgg cgaatccacc aggttgcgta
    58861 tgttgagaag cggaaaccac gttccgggtc aaacttctct accgcacgga tcagccccag
    58921 gttgccctct tcgataaggt ccagcaacgc cagaccacga ttgccataac ggcgggcaat
    58981 ttttaccacc agacgcaagt tactctcgat catccggcgg cgagaggcga catctccacg
    59041 cagtgcgcga cgcgcaaaat aaacttcttc ttcggccgtt aacagtggtg aataaccaat
    59101 ctcaccaagg taaagctgag tcgcgtccaa cacacgctgt gtggctccct gcgataacag
    59161 ttcctcttcg gccaaatcgt tatcactggg ttcctcttct actaaggcct tttcgtcaaa
    59221 aacctcaact ccgttctcat caaattccgc atcttcattt aaatcatgaa ctttcagcgt
    59281 attctgactc ataaggtggc tcctacccgt gatcccttga cggaacattc aagcaaaagc
    59341 ctggttccgc cgatttatcg ctgcggcaaa taacgcagcg ggtttacgga tttccccttg
    59401 taacgaattt caaaatgcaa gcgtgttgaa ctggttccgg tgctacccat ggttgctatt
    59461 ttttgccccg ccttcacttc ttgttgttcc cggaccagca ttgtgtcgtt atgggcgtag
    59521 gcactcaggt aatcatcatt atgtttgatg ataatcagat taccgtagcc gcgcagcgcg
    59581 ttaccggcat aaacaacacg gccatctgcg gtcgcgataa ttgcctgtcc tttgctgcct
    59641 gcgatatcaa tccccttgtt gcccccctca gaagcgccaa aggtttcgat cactttgccc
    59701 tcagtcggcc agcgccaggt ggagataggc gtactggttg atgtactgct gacagtcggc
    59761 tcggttgtgc ttgctgttgg taccgttaca ggcgctgtga ccgtggtcgc agttggcttg
    59821 ttgttcggca acattttgtt agcactctgt tcacccgaag actcagaata cgtaattgtc
    59881 ggttgcgacg caacagcaac ggtggaattt tgtgcaggct tgatcacaac tccttgctct
    59941 gctgcgtcgg cctgggtaat ggcatttccg ccagtgattg gcgtaccgga agcattaccc
    60001 acctgtaagg tctgaccgac gttcagcgcg tatggtgcct gaatattgtt gcgctgagca
    60061 aggtcacgga aatcgttgcc agtaatccag gcgatataga aaagcgtgtc gccttttttc
    60121 acggtatagg tactgccgct ataactgcct ttcggaatgt tcccatactg acggttatag
    60181 acgatgcgtc cgttttccat ctgtaccggc tgctgagcta ctggctgcac tggctggatt
    60241 tgcggttgtt gagtagcctg aatttgtggc tgctgcaccg gctgaatttg cggttgctgc
    60301 gctgtagacg tcgtccccat tttcggcggc ggcgtaatca acataccaga attggtatgt
    60361 gcaggcgcat tgccattaac ggagctgacc ggtgccggtg gatttgaagt gtcagaacag
    60421 cctgccagcc atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt gaattttggg
    60481 cttcccgcgc tcatttatcc cccaggaaaa attggttaat aaccagtgac ataattaccg
    60541 tgcaaggcac catactgaac actggaaaag atgttcacga tacgctgacc tgcggcaaaa
    60601 taaccaggaa aaatccaggt atttcctcac gttttaagcc agctcaccct tcactaaagg
    60661 gacaaagcgc acggcctcca cggtatcgat aataaattcg cctccccgac gacgcacccg
    60721 tttcaaatac tggtgctcct cccctacggg taagacgaga atcccgcctt cgtccagctg
    60781 cgtcattagc gcagttggaa tttccggcgg tgccgccgta acaatgatag cgtcaaacgg
    60841 cgcacgtgcc tgccaacctt gccatccatc gccatgacgg gttgaaacat tatgtaaatc
    60901 aagatttttc aggcggcgac gcgcctgcca ctgcaagcct ttaatccgtt caaccgagca
    60961 aacatgctgg acaagatgcg ccaggattgc cgtttgatat cccgaaccgg tgccaatttc
    61021 cagcacccgc gactgcggcg tcagctcgag taattcggtc attcgcgcca ccatatacgg
    61081 ctgcgaaatc gtctgcccct gacctatcgg caaggcgata ttgtcccagg ctttttgttc
    61141 aaacgcttca tcaacgaatt tttcacgcgg cacggcggca agtgcattca gcacctgctc
    61201 atcctgaata ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc tgcttaccat
    61261 tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca catcttgcgc gctatgcgca
    61321 gttaaatcca catgcagcgg cgtgatggag acatagccct catctaccgc agcaaaatcg
    61381 gtccccggac cggcatcaca tttaccgccc ggcgggccaa tccagtacag cgtattgccg
    61441 cgcggatctt gctgcgggat cacctgatct gccggatgtc gtgtaccgca gcgcgtcacg
    61501 cgaatacctt tgatttgatc caagggtaaa tccggaacat taatattaag aatacgcccg
    61561 gtgcgcagcg gctctttaca cagtgcgcgc aaaattgaac aggttaccgc cgcggcagtg
    61621 tcgtaatgtt tatgcccgtc aagcgagacg gcaagcgccg gaaaacctaa atgacggcct
    61681 tccatcgcgg cggctaccgt accggaataa ataacatcat cccccagatt cggcccggcg
    61741 ttaattccgg acacaacaat gtccgggcgc ggacgcatca gagcattcac gccaagatag
    61801 acgcaatcgg tcggggttcc catttgcaca gcaatatcac cattttcaaa ggtaaacgtg
    61861 cgcagggagg attccagtgt cagagaattt gaagcgccgc tgcggttacg atcgggggcg
    61921 accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat accgggtgca
    61981 tgtaccccgt catcattact cagcaatatg cgcataatca cctgttgtgt tgataagttc
    62041 cctgacaacg ctggttgcaa aactacccgc cggaagccag aaacggattt ctacggtgac
    62101 gtcatcccac caattccagc ttaattgttg cggatacagc agcatcgctc tgcgcgcggc
    62161 ttcaactttt tcgcgcacca gtaaagcttg taattcagtt tctgcggcga cagctgcctg
    62221 ttcgaatgcc agcgcttcac gctgagttcc ccattcacca ctgcccggca atgcggcggt
    62281 tatcatcagc tctttatcgt tgacgcgacg ctgtaattcc accagttctt cggtggttgc
    62341 aacaaaccag ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa cttgattaac
    62401 gtctgctttt ttgaggcgct cagcaacaat ctgattaaac aacgcactgc gggctgccga
    62461 caaccaaaaa ctccgtttat tgcgatcgcg caccggagta ttggtttgcg cccagcgcag
    62521 cgcgccctgc aagttgctac cgccaatccc aaaacgttgg gcaccgaagt agttcggtac
    62581 accttttacg caaatatcga tcagacgttg ttcaacgtca tcgcgattgc tcacttcgcg
    62641 cagaaccagg gtaaaggcgt tacctttcag cgcccctaaa cgcagcttgc gcttgtgccg
    62701 cgcatactcc agcacctggc agccttccaa ttgaaaggcg ctcagatcgg gcatttcctt
    62761 gcccggcacg cgagcgcata accactgttc tgtaacagca tgtttatctt tttgcccagc
    62821 aaagctgact tcacgggcat gaattttcag gaatttcgcc agtgcatccg ccacaaaacg
    62881 ggtattgcag ccgtttttga ggattctaac cagaatatgc tcaccttcac catcaggctc
    62941 aaagcccaaa tcttccacca ccacaaagtc ttccggattg gctttcagca gcccggtgcc
    63001 ttgcggttta ccgtggaggt aagtgagatt atcaaactca atcattttgt tgccttaatg
    63061 agtagcgcca ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc aagtttttcc
    63121 gtagtagtgg ctttcacgtt aacatcatcc atatggcagc cgagatcttc ggcaataaac
    63181 acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat agtgacatcg
    63241 acgttgccaa gggtataacc cttcgcctga atacgacgcc aggcttcgcg tagcagctcg
    63301 cggctatcgg cacctttaaa tgccggatcg gtatccggga acagtttgcc gatatccccc
    63361 agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc gccatcagaa
    63421 tgcgccagca atcctttttc gtaaggaatg cgtacgccac caatgataat tgggccttca
    63481 ccgccaaagg cgtgtacgtc aaaaccgtgt ccaattcgca ttatgtattc tcctgatgga
    63541 tggttcgggt gaggtaaaac tcggccagtg ccaaatcctc cgggcgcgtg actttaatgt
    63601 tatccgcacg gccttcgacc aactgaggat ggaatccgca atattccagc gccgaggctt
    63661 cgtcggtaat agccgcgcct tcatttagag cgcgcgtcag acagtcatgt aacagctcac
    63721 gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg gtatgagcaa
    63781 tggcattttt gcccggttcg gcacgtttca tagtatcgcg cactggtgcg gctaggatcc
    63841 ctcccgtgcg gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc tgatgcagac
    63901 aaggacgagc ggcgtcatgc accaataccc actgcgcgtc gccagcggct ttcaaacctg
    63961 ccagcacgga atcggcacgc tcatcaccgc catctacaac ggtgatttgc ggatgattcg
    64021 ccagaggaag ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg acacgtttca
    64081 cccggggatg cgccagcagc gcatacaccg agtgttcaag aatggtttga ttaccgattg
    64141 agagatattg cttaggacat tccgtttgca ttcgacggcc aaatccggcc gccggaacca
    64201 cggcgcaaac atccaaatga gtggttgcca tgttaattcc cgggctgatt tatcgattgt
    64261 tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac cagacgataa aaagtttcgc
    64321 ccggcctggt catgctgagt tcattacgcg cacgctcttc gagcgcctcc tggccgccat
    64381 tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc tttaagtttc gcgtttgtag
    64441 cttgctgtgc cgccacatca tcattgacgc gggtatagtc atgtataccg ttcttaccga
    64501 accacagcga atactgtagc cagaccagaa tagccagcaa cagcagcgtt agtttaccca
    64561 tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc gaagacgact ctacatcctc
    64621 tgttggggat accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg ttacgtatac
    64681 ccagggcgtg cagaacataa tctcattatt agttacggtt tgaattatga acagaggaga
    64741 caagaaagta caaattagcc cagtagccac ataaacagtg cgccaaacat aatgcctact
    64801 gtcatcaggg tgaaaacaat actgtagcgt agctttccgt ccatcaatga atgcagcgca
    64861 atccccacca ctaccgcaac gggcatcagc gccagaaaga aaggccaggt gtagataaag
    64921 aagaacagcg tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa taaccaggag
    64981 ataaaaccga ccacggcacc aggcagtgac catgtggttt cttcatcctc agtaaggctg
    65041 tcgttatttg ttagtgtaat gttatggcta ttacgcatat ttgatcctgt tactttgacg
    65101 aaccgggcat ggaaacccgg tggtgtctca ggatctgata atatcgttct gtctcaacag
    65161 atctaataat tgctgtacca aatttgttac taattgttca ccattgagat gaatttctgc
    65221 cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt caccggcacg
    65281 cgctttctta tataagcctt ttggatcgcg ggcttcgcaa atcgccagcg gcgtatcgac
    65341 aaacacttcg ataaagcgcc cttctcctac gcgttcgcga accatctggc gttcggcgcg
    65401 gtgtggcgag ataaatgcgg tcagcaccac cagtccggct tcaaccatca aattcgccac
    65461 ttcaccgacg cgacggatat tctctttacg atcggcatcg ctaaaaccga gatcgctgca
    65521 taatccgtgg cgaacattgt cgccatccag cagatacgta ctgacgccga gtttatgtaa
    65581 cgcctcctcc agcgccccgg cgaccgttga tttaccggac ccggagaggc cggtaaacca
    65641 cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga ccggatggct
    65701 atgccagacg acgttttcgt catgcagcgc cattatttct cccccagcaa atcgcgcgcg
    65761 ccccagtgtg gaaagtggcg gcgaaccagg gcattcaatt ccagttcgaa tgcactaaat
    65821 tcagatggcg cagcagttgc ctggctaact ggctcatgca ccataccggc acctacggtc
    65881 acattgctca ggcgatcgat aaaaatcagc ccaccggtaa ccgggttttg ctgataacga
    65941 tctaacacca gtggctcgtc aaaagtgaga tccaccaggc cgatgccgtt cagcggcagg
    66001 ttttcaactt cgcgttgggt aaggttatta atatcaacct gataacgaat gccatcaaca
    66061 cgagaacgcg tcttcttacc ggcaattttg atgtcgtaac tctggcccgg ggaaagcggc
    66121 tgttccgcca tccataccac atccaccgac gcgctctgca cagctggtaa cgcttcgtct
    66181 gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac cagggtgata
    66241 gcttctccgg caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg cgcgacgttt
    66301 gattccacac cagagggcag cacttttaca cgttgcccga cttccacgcg accggatgcc
    66361 agcgttccgg cgtagccacg aaaatcgaga tttgggcggt taacgtactg caccgggaag
    66421 cgcattggct gggcatccac cactcgctga atctccacgg tttccagcac ttcgagcagt
    66481 gtcggaccgc tgtaccacgg catactttca ctttgcgaag ccacgttgtc accttccagt
    66541 gcggagagcg gcacaaagcg gatatccaga ttacccggca gctgcccggc aaaggtcaga
    66601 taatcttcac gaatacgggt gaacgtctct tcactgtaat ccaccagatc cattttgttg
    66661 atcgccacga ccagatgttt gatccccaac agtgtggaga taaaactgtg acgacgggtt
    66721 tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagttcaca tgtcgatgcg
    66781 ccagtcgcca tattgcgggt gtactgctcg tgccctggag tgtcggcgat aataaattta
    66841 cgcttctcgg tagagaaata gcggtaggcc acgtcaatgg tgatgccctg ttcacgctca
    66901 gcttgcaggc cgtccaccag cagagccaga tccagctttt cgccctgggt gccgtgacgc
    66961 ttactgtcat tatgcagcga tgagagctga tcttcataga tttggcgggt atcgtgcaac
    67021 agacgaccaa tcagggtact tttgccgtca tcgacgctac cacaggtcag aaaacgcagc
    67081 aggcttttat gttgttgcgc aatcatccag gcttcgacgc cgccttcatt ggcgatttgt
    67141 tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcagct ccatagaacc
    67201 agcttggtcg cggtcaatca cgcgcccctg acgttcactg gtggtggaaa ccagcatctc
    67261 ttcgatgatc tccggcagcg tttgtgcatt tgactccacc gcaccggtca gcggccagca
    67321 gcccagcgta cggaaacgca ccatccgttt tttaatcact tcgcccggtt gcaggtcgat
    67381 acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggac gttccgcagc
    67441 gagatacagc ggcacaatgt cgatattttc cagccagatg tattgccaga tatcctgctc
    67501 ggtccagtta gagagcggga aaacgcggat gctttcgcct ttgttaatct gcccgttata
    67561 gttgtgccac agctccgggc gctgattttt cgggtcccag cgatggaagc gatcacggaa
    67621 agagtagata cgctctttag cacgggattt ctcttcgtcg cggcgcgcac caccgaaggc
    67681 ggcatcaaaa ccgtatttat tcagcgcctg cttcaggcct tcggtcttca taatatcggt
    67741 atgtttcgcg ctgccgtgca cgaatggatt aatccccatc gccacgcctt ccgggttttg
    67801 atgcaccagc agctcgcagc cgtaggcttt cgccgtacga tcgcggaact catacatctc
    67861 acggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac ctggataaaa
    67921 cgccttgcgc gccagatgca gcatgacgct ggaatcttta ccgatagagt agagcatcac
    67981 cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg cctccagttg
    68041 ccgcaggtga gtaagtcgta tttgatccat aaccgttcct ttgcaatacc gctattttct
    68101 tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta ccaattagaa
    68161 tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag tgcttaaggc
    68221 accggatttc gggcgtttag gaagatttga aattgtttta gcgcagcggc agtttcatac
    68281 tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc gcattgcgcc
    68341 accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac gcctcgtcac
    68401 ctaaacctgg cgattttgcc aatacacagg cgcgacatat tgccactttc tttccgggac
    68461 gaatgaccgg aacacccgca gaaatgttat ctgccgatta tattcggcaa cagtttcagc
    68521 aaatgggtta ccgcagtgat attcgtacgt ttaatagccg atatatttat accgcccgcg
    68581 ataaccgcaa aaactggcac aacgtgacgg gaagtacggt gattgccgct catgaaggca
    68641 aagcgccgca gcagatcatt attatggcgc atctggatac ctatgccccg cagagcgacg
    68701 cagatgcaga tgccaatctc ggcgggctga cgttacaagg aatggatgat aacgccgcag
    68761 gtttaggtgt catgctggaa ctggcagaac gcctgaaaaa tacgcctacc gagtatggta
    68821 ttcgatttgt ggcgaccagt ggagaagagg aagggaaatt aggcgctgag aatttactca
    68881 agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc gataacttaa
    68941 ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca gtaaggaaat
    69001 taacgcgcga cagggcgctg gcaattgcgc gtagtcatgg aattgccgca acgaccaatc
    69061 cgggtttgaa taaaaattat ccgaaaggca ctggatgttg taatgacgca gaaatattcg
    69121 acaaagcggg cattgctgta ctttcggtgg aagcgacaaa ctggaatctt gggaataaag
    69181 atggttatca gcaacgcgca aaaacagccg cattccctgc gggaaatagc tggcatgacg
    69241 taagactgga taatcagcaa catattgata aagcacttcc tggaagaata gaacgtcgct
    69301 gccgtgacgt tatgcggata atgctaccgc tggtgaagga gttggcgaag gcgtcttgat
    69361 gggttggaaa atgggagctg ggtgttctac cgcaggggcg gggaattcta agtgatatcc
    69421 atcatcgcat ccagtgcgcc cggtttatcc ccgctgatgc ggggaacaca gcggcacgct
    69481 ggattgaaca aatccctggg ccggtttatc cccgctggcg cggggaacac aaaccgaaac
    69541 acacgatcaa tccgaatatg agcggtttat ccccgctggc gcggggaaca ctttggtgac
    69601 agtttttgtc actgttttgg tgacggttta tccccgctgg cgcggggaac acactaagca
    69661 tacatatctg tttttaaaca aatttattcc acatcaacaa tctaccaact aaattcaaac
    69721 atttccttat ttttaaagaa cacataacct attgattatc aacaggaaga aaagaaacca
    69781 aacgtaaccc atccaaatcc accggaatac gtctgttttc tccccaggtc tgaaattcaa
    69841 aacccgactc ggtattggtc gcccaggcca tcaccacatt tccgcaacca gccagttggg
    69901 taatttgctg ccagatcatc tcccgaatac gttttgatgt atcaccaaca tacacaccgg
    69961 cacgcacttc cagtagccag attgcgagcc gtccacgtaa gcgcggaggg acattttctg
    70021 taacaaccac gaccatgctc atccgccgcg cccccggtga ccactatcac ccagcgtttc
    70081 aggttcaggg atggcaggcg gtaacatatc cggcgcgggt tgtggtggtt caatttcacc
    70141 tgcagcaagg actttctcaa ttaacggtat taatttgccc gttaacttag tgctacggaa
    70201 aatatcgcga caggctaatc tgacttcttt atcaggttct gcgggttgcc tcgctgctat
    70261 ttcaaatgcc tttggcacaa ccgaatcaaa tttaatgata tcggctatgt cataaacaaa
    70321 tgaaagcggt ttgccactat gaataaatcc aatagcgggc gcatatcccg cggctaatac
    70381 tgccgcttca gaaataccgt acagacatga tgtggcagca ctgatgcagc gattcacaac
    70441 atcgcctttt tcccagtctt taggatcgta tttgcgacca ttccatttca caccatattg
    70501 tttcgccagt aatgcatagg tctggcgaac gcgggatccc tcaattcccc gtagctgatc
    70561 cactgaacgg cgagctggcg gtggctcacg aaaacgtaat tcatacattt tgcgcaccac
    70621 cttcaggcgt agatcttccg ttaaagccag ctttgcctgg tagagtaatt tatctgcccg
    70681 cgcccctccg ggttgtccgg aagagtaaac gcgaacgccc gcttcaccga cccagaccag
    70741 cagtgttccc accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg ttcccggttc
    70801 gagcataatg caggcgaccg atcccaccgg aatgtgcgtg cggatcccgg ttttgtcgat
    70861 cagcacgaaa gcgccgtcca gtacgtcgat ttgaccgtac tggaggaaga tcatagaggt
    70921 gcgatctttt aacgggatcg gactcagtgg tacaaacgtc acacctctgc tccgggtttg
    70981 atcagcatca gaccacagcc gaacgcgcgg cttttgccat acccctgact gagacgttgc
    71041 agaaataacc cgggatcggt gaccgtaagc atccccgtat aatccacgct actgaactgg
    71101 atcagttgcc gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc aaccgaggta
    71161 tccagcagtg taaaaccgct tctctccccc tgcgctgcca gccagtccag cgccgcctgt
    71221 tgttgatgca accagacatc acttccttcc gcctgccccc tcacctgccg tttcgcctcc
    71281 atcagcagat cgtggcgctt gcccgcttta cagattgttg gattcgcccg caggttgaaa
    71341 caaagttgtt gtccggtacg cagctcgggc acaaatgacc ggcattcgat ggtgaacgtt
    71401 tcactttccg ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc ctggagttct
    71461 tcccgacgat aaagaaattg cctttctttg ccgccaggga agagatccca cagccactga
    71521 tgcatcacat attccccgcg atccaccaaa tgcagcaact gcgcaggcga aagctggccg
    71581 gtatgtaagg ttattcttga gaggtacatg gttcctcctt gctgagccac ggcccctgat
    71641 tgatggtgcg ttccccaaac agccactgct gacgatttaa aggaacatcc cggcgacgta
    71701 atattttgct cgcgaccagg ccgtcgtgtt ccccttccca ccagcattca tcctgaagtt
    71761 tcgggagtga gactttcagt tcgcggaaac tatcctgata ctgttggtat gcgttacgta
    71821 agacatcaga cgcgttgcct tcgagcagta acggcgcaag tggtaacgcc agcggatgac
    71881 tttttcgccc cagataaagc ggaaaaaccg gatgacgtaa accgtcctgc aactgttcaa
    71941 ggctgtaagg cgcatcgggg gttgttgcca ccgccaccat ccaccaggca tcggtgtagt
    72001 agtcgcgccg ggagataatc gcgctcagaa gatcaggggc gctcaactct tcgcgacggc
    72061 tgaaataacg cgctttacgc acctcttttg gcatctggac cgtgtgataa tcccgtgccc
    72121 agcgcgggtt acggctggcg caaaccacca gtgaatagtg gcggttaaac gcgtttaatc
    72181 gttcggtatc atcacgccga atccctaccc cggcagccag cagccccagc aatgctgagc
    72241 gcgaaggcag ttcatgggta tgacgcactt cgccgggggc atcgacgccc caggatgcca
    72301 ttggcccatg aagctgaaaa atcaaatatt ggctcattag ccgcccctta cgcgcagata
    72361 aagtccagca cgtccttcat gcttccctgt ttgttcatca cgtcaaagct tgcgcattcg
    72421 gtcttctgtt catagaccgt attcatattt tcgcgaagcg ttgtaatacg ctgcaccgcc
    72481 acatctaact gccgggtgcc attaatgggt tcatagaaag ccgccgccag agaacgtggt
    72541 tgttcggtgc ctttttctgc cagcgcccag gaggcgtagg cacggctggc aaagctgttc
    72601 tgtttgccgg ttggggagac tttaagtgcg gcttccgtaa aggcgcgcaa ggtctgatta
    72661 gctaacgctt cgtcaccgcc gaggttttcg accagcagat ctttatcgat gcagatatag
    72721 gtgtagaaca gcgcagaacc gaatccggtt tcaccaagat gcccggcacc ggcatcttca
    72781 gaagcctggc gcaaatcatc aacggcggtg aaaaaatcat cttcgacaat cgtttcactg
    72841 acaccaaatg catgcgcgac ctggcaggcg gcttcaacat taaattcggg tttattcgcc
    72901 agcatacgac caaacatagc gatatctact gccatgcgat ctttacgtaa caaagcgaga
    72961 tcttcctctt ttggcgcgcg cttttcttcg gccagttgat gggccagcgc ttttacggcg
    73021 tcaaattctg ccgggctgat atggactaat tgttcagttt cggcgttagt gagcggatct
    73081 tttggttttt tgtcgttttt agctttccca agataatccg caatttttgc cgcccattcg
    73141 atggcttttt tctcttcgat gcctttctca atcaggatag ttgccgcctc acgcgcaata
    73201 cgcccactgc gaataccaat atggcccgcc agtgcctgtt caaaaagtgc agaagtgcgc
    73261 cacgcacgtt tcagactttg cgaggaaacg cgcagtcgcg ttgctccacc caggaccacg
    73321 gttttcggcg ctccggtgtc atcacggtta aggttggctg cagggtaagc ggttaacaaa
    73381 tgaagctgaa taaatgtcgt catgagatag tcctttattg attttgttcg ttatcgacgt
    73441 cgccagcctg gtaatattcc agcgcccagc ggatacggat aaattcggtt ggccgctgat
    73501 gacggcggtg atgattgagc agatcgtcgc tctcctggca ccagcggaag acaccctctg
    73561 ccagagagtc aagattaacg gaaccgttta ataatctgac tacgcggcgt aactggcgca
    73621 gtaattcatc cggtgttttt actgccgaca ggcgagtaaa acgccccttt gacattaccg
    73681 ccgccagctg cgcagcaaaa ggcagccgtt catcaatggc tttaacatta gcgctaagtg
    73741 cggctatcag cgccagtgcg gtaatacgcc attcaggttc atcctgccac tttatttgtc
    73801 tgttcttcag aaacaggcga aatccatccg tcagacaaac atcattaacc gtcgtactac
    73861 gccgcagact ggcacgttcg ccgcgtttct cctgcaattc ctcatgccat ttgcgcagcg
    73921 tggctttgtg ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc
    73981 ggctttagca ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgac
    74041 gcgttccaaa tcaacgggct cataaggatt ggtgaataca cgctcgtcaa aatcctgacg
    74101 tgcgaataac caaatttcct tttgccattt gccgagtaat tcatccgcat cctgaccttc
    74161 ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc
    74221 cacaaaactg aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc
    74281 actccgtaac agactcagaa tccgtgaagc cgtttgcgca gccagccgca gcttcggtat
    74341 ctggccttct tttttattga gcagcagcgg gaaatggtgt tcgtaccaac agcgcgcttt
    74401 catgttgtcg aaatcataac caaatcccca caggcccact tttgcctgtt tcagactgct
    74461 ggcattaaag agtttcacca ccagcgcggg aagttccgta ttgttttctg acttacccgt
    74521 ttcgataagg cctaaccagt cgcgccagat taaaccgccc ggttgtggtt taacggagta
    74581 aaactcaccg ccctctttaa gtggtacacg gtaaggcgtt aagggatgct gccacatggc
    74641 ataattcgca ccgtaatttt tggtagtcat caaactcaga agcgcgtcac tctgctcacc
    74701 gcaaatatcg cagttgccga ctgtcgtggt attaaaatca atacgaatac gccgcggcat
    74761 tccccagtac gcctggagtt tattgacctg atcatcggtt accaccgcac cggccagttc
    74821 gctggtacgc gtcgggccaa gccaggggaa aaccagatcg tcaaattttt tgggtagcgg
    74881 taagtcggct tcatcctgcg gcatcacgtt gagccacagt ttgcgccaca agggggcttg
    74941 ttgattaccc tgatactcct gcaattcaat cagagtcgtc atcggcccac cgccgcgtaa
    75001 accggtgcga tagcctttgc cacctgacgg cgcatttaac tgtagggaga acagagctaa
    75061 cgcagaacaa tgagagcata cgtgttcagt cacgccacgc ttaataaagt ggtctttatt
    75121 aaacttcgtt gtttgagcgc cgggaatctc aggcagtagc gaagcgacct gaactttatc
    75181 gcccatgagc acctcgaaat cctgcataaa tgaaggtgaa tctgggccaa actggaaagg
    75241 gtgttctaat gacagcaatg cttcccgtag cttttcagct tccagcccgt cttcccagat
    75301 atcatcccaa cgacgataat cttttggcgc gaaactgctt tgtagtaacc ccagcaaaaa
    75361 ctgccatgcc gccccctgga gatctgcccg cggcgcagcg atatcgacaa cattttcatc
    75421 cgccagatcg actggcgcca gcttgcctgt tgttccgtct ttaaaacgaa cgggcaacca
    75481 cggggttgtc agaagtgaaa acgagttcat atgtatcctc tcgtaaaaaa gaccatccct
    75541 ggtcggttgt ccatcattcc atccttttcg gtgtatcaac tattcagaga gtcctgtcgc
    75601 aggtatctca atcaaccttg ccaatcaatc cctccctggc cgaatacccg caactttcgt
    75661 catcagtgac taaaatcacg tttgccattt ccggatcttg ccgctgttca atgcaccact
    75721 gcctgaacgc ttccccttcc agtaaagaaa actcatcccg atgttttttc caccagcttc
    75781 gacgcactct gacaacgctc atttcccatg cgtgagcacc ggtggcataa ggcttcacca
    75841 caccggcaat acaggtagcc agccacaggg aaacagattc ctcagccaga cgtgtcgaca
    75901 gcttttccgg aaggtaatcg ttgatattgg cggcatagcc aggcttgaag ttcaggacaa
    75961 actttttagc cattgcgcga tcgcagtaat atttgcccac ttgctcctgc tcgctgcggg
    76021 caaatccttc cggcattacc acgtcctcac cgtagactga ttcaataaga aggcgggctg
    76081 cgtgtggcat ttgaatagcg ccttgctcac gcagtacacg ctgcgtcagc cagattcgtc
    76141 catgatcggg atagacatat gcactgttac gcatggcact gccgaaccat tcgtcaccag
    76201 gagcgtcgtc ccagacgggg gccagaatca gcaattcagg aggggaacgc tcgtcttttc
    76261 cgtcacgctt taactgacca ttaatatcgc ggatatgccg ctgtaatcgc cccgctcgct
    76321 gaatcagcaa atcaacaggg gccaggtcgg agatcatttc gtccaggtca caatcaacgc
    76381 tctgctctaa gacctgagta caaatgagga cttttccggc acgctgtgaa ccgtcttctt
    76441 taccaaagcg tgccagcgtc tccatttcaa ttcgctggcg atcgctaaaa gcaaagcggc
    76501 tatgaaagag tgaaaggctg gaagcgggaa tgacgccgcg ggcaagcagc tgacgatgaa
    76561 ccttaatagc gtcatcgaca gaattccgga tccaggcgat gcattttccc tgacttaccg
    76621 ccgattcgat acgcgcaata ctctcttgtt cactatgaag ccaacccacg ctgacgctac
    76681 gctcaacgtc tttgcgcgtc gctacccggt gtgagttcac atcggatttc gtgacatgcg
    76741 tcagccaggg gtaatcatcc ttttcaagga acggagcttc ttgctggccc tctgtgccac
    76801 gcgcaaaggc ggcgacgagt ttgtcgcgct gctgttggga taacgtagca gaaagcaaaa
    76861 tgacgctgtt tccgccacgc gcctgccgct cgatcagccc ttcaagaatg cacgacatgt
    76921 aagcatcaca ggcatggatc tcatcagcca gcaggatttt gttactcaac cccagaagcc
    76981 gcagattatt atgtttaaac ggcatcactg ccatcatcgc ctgatccagc gtgccgacgc
    77041 caatttcagc cagtagcgcc ttcttgttac tgttggcaaa ccaggccgca catccctgac
    77101 tgaatgtttg ttcatccggt tcttctgacc cgactaaatc accggaccag agtgattcat
    77161 tgaagcggtc cattaatgtg cgggcactgt gtgccagcac caagctgggg cgggactctg
    77221 gcgaatagaa agcaagccag gttttgacca gccgatcgta catggcattg gccgttgcca
    77281 ttgttggcag gccaaaaaac aaaccctgtg ctttcctcgc agccatcaac ctgtgcgcca
    77341 ggataagcgc cgcttctgtt ttacctgcgc cagtcacgtc ttccagaata aataactgtg
    77401 gccctggctg gctgatatcc agatccagta ccttttgctg taatggtgtc gggtgctcaa
    77461 taaaaggaaa cagcgtatta attccggtga aaggtgcggt ttctgctttt ggaggaaaga
    77521 cggttaaggc gttttgagcc tgaactaaag ttttctgcca gtaatcttta atatccattg
    77581 ggtgtgcgac gcgtggaaaa aatcgcgttg acgaacccgt ccagtctgcg agtacgactg
    77641 ttgcagagat ataccaggaa agttgtttta aaagttcaac gccctcgtca tcatcccaga
    77701 atgtgggaat ctctatgagc ggaaacagtg ccttgatttc aaggagaaaa tctcgcgcgg
    77761 cagctttgtc ttcaggcaga aaattatcca gctcatcaat acggtcaggt ggtcgaccat
    77821 gatgcccggt agttatggac atccacatct ctattacacg tgtaagttta cgagaagaga
    77881 gtgaagatga aggaagcaac tcctcacatt cacttaaata ataattccac agccagtaac
    77941 ccagcgttga atgagagatc ttttcgtaat tctttctgga accttccgga atcttgagtt
    78001 caggggccag gtaaagttgc tgaaaagagc gggcaaattt tccaatatcg tgccagcaca
    78061 gcaaccaagc gaaaaattga gccgcctgtt ccttgtcaga aatccctaat tgacgaaagt
    78121 aatcagccag cccgaagcaa tttcttttaa ccattaaata gcccattgcg gccacatcca
    78181 gcgaatgcca gcaaagaagg tgatagccgt cgccaccctc tttctcgcca cgtcgggttt
    78241 ttccccagaa atcaaagaaa gtcacaatat ttttatcctt cagtaaactt aaaggatatt
    78301 tacgcatatt ttaaaaatta tctgtgatat atatcagcca ataaacattt atattatgaa
    78361 taaattaatg attttcattt gaaattcata atgataaata gagactatat attcacataa
    78421 aaaatataaa tttaaaaaag ggtacaggag accaaaccat cacaattcaa tacgcggggt
    78481 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
    78541 accacgctct ctgatactca actcacataa cgagtcgcct accatgagcg tgaatcccag
    78601 tactgtacaa cacagtacta tgattgccac aagggcatat tttgttagca tgtcttgcct
    78661 ccctgcaaag aagagactaa catccgaatt gctacgttcg aacgcgagcc tcaggttgat
    78721 agaaatatcg cctggggctt ttgtccatct ggaacctcgc gaatgcttaa cgccagacag
    78781 cctcaagcac ccgacgccat tctatacctg ataattcttc cagcggcgtt cacgtttaaa
    78841 atcaggaatg ctgctcgcaa aataaaaacg cccccatcat tactgacaga ggcgtttaat
    78901 ttatccggca atatttaccc ttcgtgtaac ccacattccc tttttaagcc aaagaaacgc
    78961 gtttcttctt ccgccatgcc gggttcccat ttacgggttg tatgggtatc gcccaccgat
    79021 aaatatcctt cgtcccataa tgggtgatat ttcaggccat tcttttgcag atactgatat
    79081 atcgttcggt tatcccagtc gataatcggc agcactttaa atacgccacg ctgaattgcc
    79141 agcaccggta aattggcgcg actgccggat tgttcacggc gcaggccagc aaaccaggtt
    79201 tgcgcgttaa gttctttcag cgcccggttc atcggttcga ctttgttgat gtcattgtac
    79261 ttttcaatgc cttcaacgcc ctgttcccac agttttccgt agcgtgcttc ctgccaggct
    79321 gcgctttcgg tagcacggta cactttcagg ttgagcttga gtttgtccgt taactcgtca
    79381 ataaagcggt aggtttccgg gaacaagtaa cccgtatcgg tgaggatcac cggaatatcc
    79441 gggcgaattt gattcaccag atgcaggctc accgccgcct gaatgccaaa gctggaagaa
    79501 agtacatatt caccgggcag attatccagc gcccaggcta cgcggccttc agcgtccagt
    79561 ttttccagtt gggcgttagt ttccgccagc gccagaatgc gatctacctt cggcagttcg
    79621 ttcagggcgt ttagatcgag tttggacata gattcctcac tgtttgcctt gcctgatgcg
    79681 acgctcacgc gtcttatcag gcctacaaga ccgggctgat ggttaatccc acaaatcgcg
    79741 cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc gtaaagtcgc cgaagccttc
    79801 acccgcttcg cgctctttcg cccagcgccc tatcagttca tcaagcgacg ccaggatttc
    79861 cggctcggtg atgttttctt tatacatccg tgggatacgt gtcccaatgc ggttgccgcc
    79921 aagatgcagg ttgtagcgac ccggcgcttt acccaccagg cccacttccg ccagcatcgc
    79981 gcgaccacaa ccgttcgggc agcctgttac acgcatcacg atatgctcat cgctgacacc
    80041 atgtttcgcc attaaattat cgatgttgtc gataaaagac ggcaggaaac gctccgcttc
    80101 cgccatcgcc agcgggcaag tcgggaacga gacgcaggcc attgagtttt cacgctgcgg
    80161 cgtgacggca ttcattaacc cgctctcttt ggcgatcttc tcgatcttcg ctttctcgct
    80221 ttccggcaca ccggcgatga tcagattctg gttcgccgta atgcggaaat cacctttgtg
    80281 gatcttcgcg atctccagca ggccggtttt cagcggacgc cccggataat caaggatgcg
    80341 accattttcg ataaacagcg tcaggtgcca gttatcatca atgcccttaa cccagccaat
    80401 acgatctccg cgtccggtga actcatacgg acggatcggt tcaaatttga tccccgcgcg
    80461 acgctccact tccgctttaa acgtctcaac ccccacgcgc tccagcgtgt atttggtttt
    80521 ggcattttta cgatcggttc ggttacccca gtcacgctga gttgtcacga cggcttccgc
    80581 cactgccagc gtatgctcca gcggcagata gccaaactca ctcgccgtgc gggcgtaggt
    80641 tttcttgttg ccgtgttcga tagaaagtcc accgcccacc aacaggttaa agcccaccag
    80701 cttgccgttt tcggcgattg ccacgaagtt catgtcgttg gcgtgcagat cgatatcgtt
    80761 ctgtggcggg atcactaccg tggttttgaa tttacgcggc aggtaggtct ggccgaggat
    80821 cggttcttca tcggtggtgg cgactttttc ctgatcgagc cagatctccg catacgcgcg
    80881 ggtacgaggc agcagatgtt cagagatttt cttcgcccac tcgtacgctt ctgcgtgcag
    80941 ctgcgactcg taagggttcg aggtgcagag tacgttacgg ttcatgtcgt tagcggtcgc
    81001 cagcgcatca agaccgaccg agtgcagcat ctggtgcacc ggtttgacgt tctttttcag
    81061 aatgccgtgg aactggaatg tctggcggtt ggtcaggcga atgctgccat agatggtgtt
    81121 ttcaccggca aatttatcga tcgcctgcca ctgtttggtg gtgataactc cacccggcag
    81181 acggcagcgc aacagcatcg catggcgcgg ctccagcttc tgttcagcac gttcggcgcg
    81241 gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg atcagcagga agttatcgcc
    81301 cttaaagccg ccggtcaggc cgtcgtttaa atcttccgca atggtgccgc gcaggtagtt
    81361 gctttcaagc ttcatgcgct cggcgtctgt cagttttcct tcgaccacta aaggccctgg
    81421 atgtttttcg ctcattagta gacatctcgc tgataacggc gctctacgcg cagctcactt
    81481 aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt cggcaatcac ttccagaagt
    81541 gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc agacataaat gtgggcaccg
    81601 tcattgatcc agcgccacag ctccgcgccc tgttcgcgca gtttgtcttg tacgtaaact
    81661 ttttcttttt gatcgcgcga ccaggcaaga tcgatacgcg tcagcacgcc ctctttgacg
    81721 taacgctgcc actccacctg gtagaggaaa tcttccgtaa agtgcgggtt gccaaagaac
    81781 agccagtttt tacccggcgc ttcgtcagcg gcgcgctgct gcataaaggc gcggaacggc
    81841 gcgatgccgg tgcctgggcc aatcataatc accggggttt ccgggttagc gggcaggcgg
    81901 aagttatcgt tatgttcgat aaatacgcga acttcgccct cttcttccac gcggtccgcg
    81961 aggaagctgg aggcaccacc ggcacgggcg cggccttcca cgtcgtaacg caccacacca
    82021 acggtgacgt gtacttcgtt ctcgacttcc tcctgcgagg aggcgatgga atacagacgc
    82081 ggcgtcagcg ggcgcagcag attaatcagc gcttcggcgt ccagttgcgc cggagagaag
    82141 cgcaccatgt cgacaatcgg cgtagtcgcg gcgtaatgct gtaacttcgc tttatcgccc
    82201 accagcggca acagcgtttc gctgcgggta agcgtggcat agttctcaac aatattggcg
    82261 gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt tcagaggcaa cgttttgccc
    82321 tcgacggtga caggttcatc gcctttcagc cacagcagtt cgacaagttc tttcaccagt
    82381 gccggatcgt tctgatacca gacacccagc gcgtcacccg gctggtaacg caggcccgag
    82441 tcacctaagt caatttcgat atggcgaacg tctttttcag agttacgccc ggtaattttc
    82501 tggttaaccg aaaggctcgc taccagcggc gcgtctttgc tgtacgggct ggtgtggatt
    82561 tcatttacca cgccagtagc gacggattgc gaaggtgccg cgacaggcgc acgcgattta
    82621 agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag cctggtattc aacgtcggcg
    82681 tcgacacggt cgagcaggcg ttcaccgccc agttccgcca gcttgctgtc gaaatctttc
    82741 ccggactggc agaaaaattc ataagaacta tcgccgaggc taaacacggc aaaagcggta
    82801 ttttccagct tcggcgcttt tttggagaac aggaacttat gcagcgcgac ggcttcttcc
    82861 ggcggttccc cttctccttg cgttgacgtc accacgataa gcagtttttc gctggcgatt
    82921 tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt tcagttttgc cgtcaataaa
    82981 tcatcgcgta atgcttcagc aacccggcgc gcattgccgg tttgcgaagc agagataata
    83041 gttatacccg gcatttctgc ggctggcgct ggcgtcgctg caagcgcagc aggctgctga
    83101 ttgagcacgc cccagaaata gccagaaacc caggcaagct gggtgggagt taaatcggtc
    83161 gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg gaagcaacgc ggaaggtggg
    83221 acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag ctgtttctgc gccctgtcag
    83281 cgcccataaa acagaagaga aggtaaggtt aacggggtga acggtgtgga ttaaagacgg
    83341 gatagcgata actaataacc aaatcgacta acctgtttta gcaatagtct ttaacaacaa
    83401 aatagattaa ccaacctaat gaaaaacaaa tgaatttagc caatcattaa gataaatcag
    83461 cgattttgcg caacaaagtc gttttagata atgcgaaaaa agagcctttc cggtactcta
    83521 cggcggtttt atcgtcctgt agagaaatta tgatgtccac cacgttattt aaagatttca
    83581 ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc
    83641 acgggcattc ctttatggtg cgactggaaa ttaccgggga agtcgatccg catacgggct
    83701 ggattatcga tttcgctgaa ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc
    83761 accattatct caatgatatt ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat
    83821 ggatttggga tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa
    83881 cctgcaccgc tggttgtatc tatcgcggcg aatgataaga gtgtggcggc ggtcaatttc
    83941 ccttaagtaa cgctatgtta gggtgttgtg ttcaggatat ctggggcatg acatggaaga
    84001 cgactgcgac attattatta ttggtgccgg tattgcaggc accgcttgcg cgttacgctg
    84061 cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa
    84121 tctttccggc gggcggttat atacccatgc actcgcggaa ctcctccctc aatttcatct
    84181 gaccgcgcct cttgaacgac gcatcactca cgaaagcctt tccctgttaa cgccggattg
    84241 cgcaacgacg ttttccagct tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg
    84301 gttcgatccg tggctggttg ccgaagccga aaaagaaggt gtcgaatgca tccctggtgc
    84361 gacggtggat gcactgtatg aagaaaacgg cagggtgtgt ggtgtcattt gtggtgacga
    84421 tattctccgc gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg
    84481 tcacgggtta gtgactcgtc ctgctggcga agcgatggcg ttggggatca aagaagtgct
    84541 gtcgctggaa acatccgcta ttgaagaacg ttttcatctg gagaataacg aaggcgcagc
    84601 gttgctgttc agcggcggaa tctgtgatga cttacccggc ggcgcatttc tttatactaa
    84661 tcaacaaacg ctctcgttag ggattgtttg cccactctct tcccttacgc aaagtcgtgt
    84721 tccggcaagc gagctgcttg ctcgctttaa aacgcatccg gcagtgcgcc cgcttatcaa
    84781 aaacacggaa tcactggagt atggtgcgca tctggtgcca gaaggtggct tgcacagtat
    84841 gccggtacaa tacgccggta acggctggct gctggtgggc gatgcgttgc gcagttgcgt
    84901 caataccgga atttctgtgc gcggcatgga tacggcgcta actggcgcgc aggcggcggc
    84961 acaaacactg ataagcgcct gccagcaccg cgagccgcaa aatctgtttc cgctttatca
    85021 tcacaacgta gagcgcagcc tgctgtggga tgttctacag cgttatcagc atgttccggt
    85081 gcttttgcaa cgcccgggat ggtaccgtac gtggcctgcg ttaatgcagg atatttcccg
    85141 cgatttatgg gatcagggtg ataaacctgt tccaccgctg cgccagttac tctggcgtca
    85201 tttacgtcgt catggcttgt ggaatctggc gggcgatgtt atcaggagtg ttcgatgtct
    85261 gtagcccgta atctctggcg cgctgctgat gcgccgcaca ttgttccggc tgactccgtt
    85321 gagcgtcaga tggtacaacg gttgattaac gcctgtccgg taggtctttt ttcgctcaca
    85381 ccggaaggtg acttacgcgt tgattatcac ggctgcctgg agtgtggcac ctgccgtttg
    85441 ctgtgcgacg aatccacact acaacagtgg cgctatccgc cttccggatt cggcatcacc
    85501 taccgatttg gataaataat aaggataatt tatgcccctc ttacacctgc tccgccagaa
    85561 tccggtgatt gctgccgtta aagataatgc cagcctgcaa ctggccatcg attctgaatg
    85621 ccagtttatt tccgtcttgt acggcaatat ctgcaccatt agtcaaatcg ttaaaaagat
    85681 taagaacgct gggaaatatg cttttattca tgttgatttg ctggaaggcg cgtcaaataa
    85741 agaagtggtg attcagtttt tgaaactggt gaccgaggcg gatggaatta tcagcaccaa
    85801 agcatcgatg ctaaaagcgg caagagcgga aggttttttc tgtattcatc gcctgtttat
    85861 tgttgattcg atttcgtttc acaacattga taagcaagtt gcgcaatcga atccggattg
    85921 tattgagatc ctgccaggct gcatgcccaa agtgctgggc tgggtgacgg aaaaaatccg
    85981 ccaaccgctg attgctggcg ggctggtatg cgatgaagaa gatgcgcgta atgcgattaa
    86041 cgcgggtgtc gtggcgcttt ccaccacgaa taccggagtc tggacgttag cgaaaaaatt
    86101 gctttgacgg gataatcgta accaattgaa tttggtttga tttgtaggcc gcacgccaca
    86161 tccgacattc agcgcctgat gcgacgctta acacgtctta tcaggcctac aggtccccag
    86221 tccagaaccg taggcaggat catccggcag cacgttattg gcattctgcg tcaatgtttg
    86281 tcaccagtgc ctccagcatc actttccagt catcaaccac gccaacatct gcctgtgaaa
    86341 ataccgctgc gctggcgtca tgattaatcg ccaccacaaa cttgctattg cgtacgcccg
    86401 ccattaacgc tgctgcgcca gaagcgccaa caacaataca cacctcaggt gccagcagat
    86461 gtccggaaat accaatcact ttttcagcat caacgccacc gttcatcact cgcgcccgac
    86521 tgtagcccac ttcggctccc agcttttatg ccagcatagc gatttcctgg ttatctgcct
    86581 ctccgccctg ccccaccacc aacacccgtc tggcctcagc taatgggtcg cgggtaacat
    86641 ttttcaggtt ctctatgcta accagccagt ccggaagcgc acctgggact atgttcagtt
    86701 gctgcatacc agagggcaac gtggcatttt tatctgctcc cgcctggcgc gccagggaaa
    86761 gacatagcgg gcgtttttct gtttgcagcg tcgccgttag cgcatttccc cagtgggatt
    86821 tgcgcacgct tacgattgga atctctaacg aagtcacctg gcagatgctt gcaccatgta
    86881 aacgccaggc cagccgagtc gacagttcat cgccaaacgt tcccggcgga aagagaacaa
    86941 cgttcgcagg cgttcgctgc cactgttcca ccagcgcatc cagcacctgt tccaccacta
    87001 cgggctgtgg ttctatttgc cagtgcgcca gggtacaacc gctgaaatcc tgcgccgcca
    87061 gccagcccgc taatgcggca ttctcctgat ttattgtcac aattgcgata ttcatggctg
    87121 catcctttgg cgtaaatagt cctgccatag cttttgcgct ttttctgcta ccgtttgccc
    87181 atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc agttgcagac actgcatcgc
    87241 tggcaattcg gcagcaacag ttttacggat aatttccgct ttccccgcag ccattcgctg
    87301 gcgcattccc ggtactggca aggccacttc accgcactga cgcacggcaa tcaccgcagg
    87361 caagcgaatc cgacaacagc gcagcccatg ttcagtacgc tgttcgaggg tgataaacgg
    87421 cgcgtcgaga gtgaaacgct ccacctgggt gaagcaaggc cagcccagca tttccgccag
    87481 caaaaatggc gtttgcccat tctgcccttc gctgctttgg cagccggtaa ttatcagatc
    87541 cagcggattc tgacgctgcc attcggcaat atggcgagcg acaaattccg acgcaaagcg
    87601 cagatctgcc gccgtctcca gcaatacagc ttcctcaaac ccaagagcca tgagatagcg
    87661 cagccagtgc aacgcccgtt catcccccat gctcaacgcg gttaaagaca tcggcgtacc
    87721 gttttttctc tgcgccaaca gcagcgcggc ggcagcctgt tcatcagcac cgagtaaact
    87781 tcgcagtagc gaaatatccg gtccgctatt accatgagcc gccgcctgcc actctttttc
    87841 cgccagcatt ccggcatccg gttcggcttt aaacgctaac agaatgttca ttcgcacctc
    87901 ctacatttcc cgccgccacc agcgggagtg ctttggtttc tggtgcccat agccaggtta
    87961 caacaaaacc agccaacaac actgccgcca ggagcaataa ggtgacttgc attccccact
    88021 gcgccagcac ccacggcagc aggccagtac ttaccgccgc ccccaaccga ctcatggcgg
    88081 tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc
    88141 ccaccagatt actgactgcc gaaatggtgg tgctgaagag aacaaaagca gcagcgttaa
    88201 tgaactgccg gaaggcaaac aggccatcac taccagcgtt gccgccagca gcaaaaaact
    88261 tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc
    88321 cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca gtccaatagt
    88381 ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga ggcagacaaa
    88441 gaagacgctg ttaaacgccg tgcgccgcca gtagcgcgaa gagaacaagg ttttgatgtg
    88501 tttatgggtc gccgttgcca cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg
    88561 cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gcgattctgg
    88621 cgttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg ccagcagcca
    88681 gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg gcaatacttg ccaacacata
    88741 gcctacggtc cacaccacgc tgaatgcgcc cagcaaaaca ccgcgatggc ggcgcgggga
    88801 aaattcagcc agcaaggtgt gacctactga gtaatcgcct cccagaccaa tgccgatcaa
    88861 aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgcaaga acgaagcgag
    88921 cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt cggagatcca
    88981 cccaagaacc aggctgccaa gaaacaaacc aaggtgcgcc gagccgccga tcattcccgc
    89041 cataaacggc gtcagttgca tggcgggcgt aagttgaata atggcgtaac caatgacgcc
    89101 gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaattc gacagtgaaa
    89161 acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt
    89221 gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc ccatcatcgc
    89281 ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat tcataatgcc
    89341 attaggatcg aactgctttt tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag
    89401 cttgctccag tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg aaccaccgag
    89461 gcgaatggtt tcttcgcaaa tgattttgtt gagcggattg tggtacttgt cgatttcctc
    89521 ttccggctta cagttgacaa cgttgtagtc gtagacgaag tacatgttgg tgccgttctg
    89581 atagctatga gaggagtgac cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcggt
    89641 acgaatacgg ttaataacgc tttcgtagat ttcgtggatg cagctccagc agccggacac
    89701 ttcggtagta aagcccatgt tgccggtttt gaggatctgc acacgttcgg cagccacttt
    89761 atccggtccc cagttcaggt tgttaaacca ggtttcgatc agcttgctgt ccacgcgttg
    89821 gcattgcggg tagcgggcaa cgatttccgc aatcccttcg cccgtcgcct tcgcaatgcg
    89881 agggttacct tcagccataa agatcagcac gcattttccg tcggcaaaat gggtgaagtg
    89941 ttgggtgcca tcttcagcgt catacaaacg agcgatcgac ggacgatacc cttccaccat
    90001 gatttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc cgtagaagag
    90061 gttgttttcc ggggtgaatt taaagatttt cactgttact tcagtgatat agcacaatgc
    90121 accttcgttg ccgatgatga tgtgacgaat gtccgggcca gccgcgcggc gtggcacgtt
    90181 tttaatgcgt gtgacggtgc catctgccaa cactgcctcc agaccaacga ccatatcttc
    90241 gattgcgccg tagagtgtgg agaactgccc gatactgcgg gttgctacca ggccgcccat
    90301 ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt cacgcaacgc
    90361 gttttccagc acctccagcg gaacaccaca ttgcgccgtc gcctgcatat tctcaatatc
    90421 aatattaatg atttgattca tggcggaacc gtcgagcacc accgagtttt ctacaacagt
    90481 ttccagccca ccttcggtgg cggaagcacc ggtacgcggc acaccgttaa ttttgtgcgc
    90541 attcataaaa ttcagcacac gggatacttg ctctgtggaa ccgagtttta cgaccgctgc
    90601 cggaatcggc aaagtataaa tgccatgaat atccggaaat ttacgaaaac ggtcaatact
    90661 gttcttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag
    90721 ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat aataaaaatg
    90781 gaataatcat taataagaga aaacagaaac caccgttatg ggttagcgca ctaaataacc
    90841 gccatcgacc actaataaat gcccgttgac ataattcgat gcctgacttg cgagaaacac
    90901 ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat gatcaagaac
    90961 gcgctgattg gtttctggat tactgcgtgt cgccagcgta atgtctgttg cataatagcc
    91021 aggggcgata ccatttacct gaatattata ttgacctagt tcatcacaat aagctttggt
    91081 gaatccggca agagcatgtt tagtagcaga atatgcaggt gaccattgtc cacctaagta
    91141 agagaacaat gaacagatat taatgatttt accgcttttc tgcgggatca taatttttgc
    91201 cgcttcgtag cttaactcga atgcggcggt caggttcaca tcaatcatcg ggtcccagtc
    91261 ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt taaccagaat
    91321 atcaactgta ccgaaacgct cacagcaggc agcgataatc ttctgcggcg cgccttctgc
    91381 ggtgatatcc acctgcatga agtccacctc aacaccctga ttttcaatca tttcctttgt
    91441 ttcgccgtta tctttgacaa aacttggaat aaagacattt gcgcctgctt tggccaacgc
    91501 catggcaaat gcctggccta aaccgctatt cccgccggta acaattgcgg ttttaccttt
    91561 cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
    91621 tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
    91681 cattatctct ggtaactgga gataattttt agcataagga cataactgct aatgtgatcg
    91741 taatcacagt gtgatattcg tattatcaaa tagttttaaa aagcagaagc gtgattactc
    91801 tcacacaaca cctctgcttt ccatgttatt tattatttca attactagag acctgagaaa
    91861 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
    91921 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
    91981 ggcggcgtta gtttcgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
    92041 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggct
    92101 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
    92161 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
    92221 tgggtaatgc tctgtattca ggtcgccttt gcgataacaa cgattttaat gctgtggtcg
    92281 gtgtcgatta aagccgcatc gttgcttggc gatcatagcg agcaagggaa aattatgggc
    92341 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cagtgtttac catgtgggtt
    92401 ttttctcgct ttgcaccgga tgacagcacc agcctgaaaa cggtcattat catctatagt
    92461 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
    92521 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct tgccgttttg
    92581 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gcgtcttcac catctacgcc
    92641 attctgagtt actccaccaa ctatctgacc gaaatgtatg gcatgtcgct ggtggcggcg
    92701 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggcggc
    92761 attatcacca cctacagtaa agtgaaatcc cctacccgcg tgatccaaat cctttccgta
    92821 ctcggcctgc tggcgttaac tgccctgctc gtcacgaact ctaacccgca atcggtcgcg
    92881 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
    92941 tgggcctgcc ctggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
    93001 tgctcggtga ttggattcct gccggatgtc ttcgtttacc caattatcgg ccactggcaa
    93061 gacacccagc ccgcagcaga agcctaccgc aatatgtggc tgatgggcat ggcggcgctt
    93121 gccatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
    93181 cccgcaatgg ccagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaga
    93241 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg tacgatctgg
    93301 aaggtaacgt ggtttgcgaa ggcaaaggtt tattgcagcc gatgcacacg ccagatgccg
    93361 ataccgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc ggtcacgatt
    93421 tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg ggatccatcc
    93481 gttgctgccg tgcgttattg aaagccgatg gcacgcctgc tgcgccgttg attagctggc
    93541 aggatgcacg cgtaacgcgt ccctacgaac acactaatcc tgacgtggcc tatgtcacct
    93601 ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat atcgccaact
    93661 attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat gctgcggtaa
    93721 tggagaagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcca ggcactgttc
    93781 ttggacagat cacaccacaa gccgcgctgg cgacacattt cccggcaggg ctgccggttg
    93841 tttgtaccac cagtgataaa ccggtagaag ctctgggagc cggattactg gatgatgaaa
    93901 cggcggtgat ttcactcggt acttatatcg cgctgatgat gaacggcaaa gccttgccga
    93961 aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
    94021 gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg ttaggcgagt
    94081 cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc aacaaaaaag
    94141 cttcttgtgt gccacctggc tgtaatgggc tgatgacggt gctggactgg ctgaccaatc
    94201 cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg gattacgcat
    94261 ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat tacgacaata
    94321 tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggtggc ggttcgaaca
    94381 gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga cgtaacgcca
    94441 ttaacggttg tgcaagtctg ggggcagcga tcaatacggc ggtaggtctg gggctatacc
    94501 cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa agatatcttt ataccgattg
    94561 agagcaatgc caaacgctac gacgcgatga ataaaggtat tttcaaagcc ctaaccaaac
    94621 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaatgcgg
    94681 attcgatcca gagctggtcg aatgcgtaag tgaggaagac cgggcgggaa actgcccggc
    94741 ctgaacatac ctgaatggtt atccccgctg gcgcggggaa cataagttcg acaacttaaa
    94801 aaatgataac aagtccggtt tatccccgct gatgcaggga acataataaa aactttaact
    94861 agttcggttt atccctgttt ttacaaggaa tattaactac actcgatgtc tttagaataa
    94921 aagacaatac gaatcgcact ttatcaattt tcacttaaag tatgaactgg atatctttat
    94981 tcataagaac tattcatcaa cagcgtatag aggcggttat gtaaaaccac tcattagcct
    95041 caaaacaaag acaa
//
LOCUS       DAZFTN010000007        81552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFTN010000007 DAZFTN010000000
VERSION     DAZFTN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..184
                     /gene="yhfL"
                     /locus_tag="ACHKV2_000607"
     CDS             17..184
                     /gene="yhfL"
                     /locus_tag="ACHKV2_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO6938546.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            480..1817
                     /gene="frlA"
                     /locus_tag="ACHKV2_000608"
     CDS             480..1817
                     /gene="frlA"
                     /locus_tag="ACHKV2_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO6938547.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1838..2860
                     /gene="frlB"
                     /locus_tag="ACHKV2_000609"
     CDS             1838..2860
                     /gene="frlB"
                     /locus_tag="ACHKV2_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO6938548.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2911..3738
                     /gene="frlC"
                     /locus_tag="ACHKV2_000610"
     CDS             2911..3738
                     /gene="frlC"
                     /locus_tag="ACHKV2_000610"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO6938549.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3738..4523
                     /gene="frlD"
                     /locus_tag="ACHKV2_000611"
                     /pseudo
     CDS             3738..4523
                     /gene="frlD"
                     /locus_tag="ACHKV2_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4623..5354
                     /gene="frlR"
                     /locus_tag="ACHKV2_000612"
                     /gene_synonym="yhfR"
     CDS             4623..5354
                     /gene="frlR"
                     /locus_tag="ACHKV2_000612"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO6938550.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5485..6489)
                     /gene="trpS"
                     /locus_tag="ACHKV2_000613"
     CDS             complement(5485..6489)
                     /gene="trpS"
                     /locus_tag="ACHKV2_000613"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO6938551.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6482..7240)
                     /gene="gph"
                     /locus_tag="ACHKV2_000614"
     CDS             complement(6482..7240)
                     /gene="gph"
                     /locus_tag="ACHKV2_000614"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO6938552.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7233..7910)
                     /gene="rpe"
                     /locus_tag="ACHKV2_000615"
     CDS             complement(7233..7910)
                     /gene="rpe"
                     /locus_tag="ACHKV2_000615"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO6938553.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7928..8764)
                     /gene="dam"
                     /locus_tag="ACHKV2_000616"
     CDS             complement(7928..8764)
                     /gene="dam"
                     /locus_tag="ACHKV2_000616"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6938554.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8871..10157)
                     /gene="damX"
                     /locus_tag="ACHKV2_000617"
     CDS             complement(8871..10157)
                     /gene="damX"
                     /locus_tag="ACHKV2_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO6938555.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10249..11337)
                     /gene="aroB"
                     /locus_tag="ACHKV2_000618"
     CDS             complement(10249..11337)
                     /gene="aroB"
                     /locus_tag="ACHKV2_000618"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO6938556.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11394..11915)
                     /gene="aroK"
                     /locus_tag="ACHKV2_000619"
     CDS             complement(11394..11915)
                     /gene="aroK"
                     /locus_tag="ACHKV2_000619"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO6938557.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12316..13554)
                     /gene="hofQ"
                     /locus_tag="ACHKV2_000620"
     CDS             complement(12316..13554)
                     /gene="hofQ"
                     /locus_tag="ACHKV2_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO6938558.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13466..13870)
                     /gene="hofP"
                     /locus_tag="ACHKV2_000621"
     CDS             complement(13466..13870)
                     /gene="hofP"
                     /locus_tag="ACHKV2_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO6938559.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13860..14300)
                     /gene="hofO"
                     /locus_tag="ACHKV2_000622"
     CDS             complement(13860..14300)
                     /gene="hofO"
                     /locus_tag="ACHKV2_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO6938560.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14284..14823)
                     /gene="hofN"
                     /locus_tag="ACHKV2_000623"
     CDS             complement(14284..14823)
                     /gene="hofN"
                     /locus_tag="ACHKV2_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO6938561.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14807..15601)
                     /gene="hofM"
                     /locus_tag="ACHKV2_000624"
     CDS             complement(14807..15601)
                     /gene="hofM"
                     /locus_tag="ACHKV2_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO6938562.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15721..18273
                     /gene="mrcA"
                     /locus_tag="ACHKV2_000625"
     CDS             15721..18273
                     /gene="mrcA"
                     /locus_tag="ACHKV2_000625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO6938563.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18438..18998)
                     /gene="nudE"
                     /locus_tag="ACHKV2_000626"
     CDS             complement(18438..18998)
                     /gene="nudE"
                     /locus_tag="ACHKV2_000626"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO6938564.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19318..21453
                     /gene="igaA"
                     /locus_tag="ACHKV2_000627"
     CDS             19318..21453
                     /gene="igaA"
                     /locus_tag="ACHKV2_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO6938565.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21518..22186
                     /gene="yrfG"
                     /locus_tag="ACHKV2_000628"
     CDS             21518..22186
                     /gene="yrfG"
                     /locus_tag="ACHKV2_000628"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO6938566.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22197..22598
                     /gene="hslR"
                     /locus_tag="ACHKV2_000629"
     CDS             22197..22598
                     /gene="hslR"
                     /locus_tag="ACHKV2_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO6938567.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22623..23501
                     /gene="hslO"
                     /locus_tag="ACHKV2_000630"
     CDS             22623..23501
                     /gene="hslO"
                     /locus_tag="ACHKV2_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO6938568.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23564..25288)
                     /gene="yhgE"
                     /locus_tag="ACHKV2_000631"
     CDS             complement(23564..25288)
                     /gene="yhgE"
                     /locus_tag="ACHKV2_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO6938569.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25667..27289
                     /gene="pckA"
                     /locus_tag="ACHKV2_000632"
     CDS             25667..27289
                     /gene="pckA"
                     /locus_tag="ACHKV2_000632"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO6938570.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27365..28717)
                     /gene="envZ"
                     /locus_tag="ACHKV2_000633"
     CDS             complement(27365..28717)
                     /gene="envZ"
                     /locus_tag="ACHKV2_000633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO6938571.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28714..29433)
                     /gene="ompR"
                     /locus_tag="ACHKV2_000634"
     CDS             complement(28714..29433)
                     /gene="ompR"
                     /locus_tag="ACHKV2_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO6938572.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29661..30137
                     /gene="greB"
                     /locus_tag="ACHKV2_000635"
     CDS             29661..30137
                     /gene="greB"
                     /locus_tag="ACHKV2_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO6938573.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30234..32555
                     /gene="yhgF"
                     /locus_tag="ACHKV2_000636"
     CDS             30234..32555
                     /gene="yhgF"
                     /locus_tag="ACHKV2_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO6938574.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            32993..33220
                     /gene="feoA"
                     /locus_tag="ACHKV2_000637"
     CDS             32993..33220
                     /gene="feoA"
                     /locus_tag="ACHKV2_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO6938575.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33237..35558
                     /gene="feoB"
                     /locus_tag="ACHKV2_000638"
     CDS             33237..35558
                     /gene="feoB"
                     /locus_tag="ACHKV2_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO6938576.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35558..35794
                     /gene="feoC"
                     /locus_tag="ACHKV2_000639"
     CDS             35558..35794
                     /gene="feoC"
                     /locus_tag="ACHKV2_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO6938577.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            35997..36875
                     /gene="rpnA"
                     /locus_tag="ACHKV2_000640"
     CDS             35997..36875
                     /gene="rpnA"
                     /locus_tag="ACHKV2_000640"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO6938578.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36902..37672)
                     /gene="bioH"
                     /locus_tag="ACHKV2_000641"
     CDS             complement(36902..37672)
                     /gene="bioH"
                     /locus_tag="ACHKV2_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO6938579.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37710..38393
                     /gene="gntX"
                     /locus_tag="ACHKV2_000642"
     CDS             37710..38393
                     /gene="gntX"
                     /locus_tag="ACHKV2_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO6938580.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38452..39027
                     /gene="nfuA"
                     /locus_tag="ACHKV2_000643"
     CDS             38452..39027
                     /gene="nfuA"
                     /locus_tag="ACHKV2_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO6938581.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39388..40704
                     /gene="gntT"
                     /locus_tag="ACHKV2_000644"
     CDS             39388..40704
                     /gene="gntT"
                     /locus_tag="ACHKV2_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6938582.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40749..42833)
                     /gene="malQ"
                     /locus_tag="ACHKV2_000645"
     CDS             complement(40749..42833)
                     /gene="malQ"
                     /locus_tag="ACHKV2_000645"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO6938583.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42843..45236)
                     /gene="malP"
                     /locus_tag="ACHKV2_000646"
     CDS             complement(42843..45236)
                     /gene="malP"
                     /locus_tag="ACHKV2_000646"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO6938584.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45848..48553
                     /gene="malT"
                     /locus_tag="ACHKV2_000647"
     CDS             45848..48553
                     /gene="malT"
                     /locus_tag="ACHKV2_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO6938585.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48620..48898
                     /locus_tag="ACHKV2_000648"
     CDS             48620..48898
                     /locus_tag="ACHKV2_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO6938586.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            48889..49374
                     /locus_tag="ACHKV2_000649"
     CDS             48889..49374
                     /locus_tag="ACHKV2_000649"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6938587.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49424..50452)
                     /gene="rtcA"
                     /locus_tag="ACHKV2_000650"
     CDS             complement(49424..50452)
                     /gene="rtcA"
                     /locus_tag="ACHKV2_000650"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO6938588.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50456..51682)
                     /gene="rtcB"
                     /locus_tag="ACHKV2_000651"
     CDS             complement(50456..51682)
                     /gene="rtcB"
                     /locus_tag="ACHKV2_000651"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO6938589.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            51870..53468
                     /gene="rtcR"
                     /locus_tag="ACHKV2_000652"
     CDS             51870..53468
                     /gene="rtcR"
                     /locus_tag="ACHKV2_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO6938590.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(53450..54208)
                     /locus_tag="ACHKV2_000653"
     CDS             complement(53450..54208)
                     /locus_tag="ACHKV2_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO6938591.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(54225..55055)
                     /gene="glpG"
                     /locus_tag="ACHKV2_000654"
     CDS             complement(54225..55055)
                     /gene="glpG"
                     /locus_tag="ACHKV2_000654"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO6938592.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(55100..55426)
                     /gene="glpE"
                     /locus_tag="ACHKV2_000655"
     CDS             complement(55100..55426)
                     /gene="glpE"
                     /locus_tag="ACHKV2_000655"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO6938593.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55616..57121
                     /gene="glpD"
                     /locus_tag="ACHKV2_000656"
     CDS             55616..57121
                     /gene="glpD"
                     /locus_tag="ACHKV2_000656"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO6938594.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(57336..57938)
                     /locus_tag="ACHKV2_000657"
     CDS             complement(57336..57938)
                     /locus_tag="ACHKV2_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938595.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(57938..58930)
                     /locus_tag="ACHKV2_000658"
     CDS             complement(57938..58930)
                     /locus_tag="ACHKV2_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6938596.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(58956..60464)
                     /locus_tag="ACHKV2_000659"
     CDS             complement(58956..60464)
                     /locus_tag="ACHKV2_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6938597.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(60589..63036)
                     /gene="glgP"
                     /locus_tag="ACHKV2_000660"
     CDS             complement(60589..63036)
                     /gene="glgP"
                     /locus_tag="ACHKV2_000660"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO6938598.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(63055..64488)
                     /gene="glgA"
                     /locus_tag="ACHKV2_000661"
     CDS             complement(63055..64488)
                     /gene="glgA"
                     /locus_tag="ACHKV2_000661"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO6938599.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(64488..65783)
                     /gene="glgC"
                     /locus_tag="ACHKV2_000662"
     CDS             complement(64488..65783)
                     /gene="glgC"
                     /locus_tag="ACHKV2_000662"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO6938600.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65801..67774)
                     /gene="glgX"
                     /locus_tag="ACHKV2_000663"
     CDS             complement(65801..67774)
                     /gene="glgX"
                     /locus_tag="ACHKV2_000663"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO6938601.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67771..69957)
                     /gene="glgB"
                     /locus_tag="ACHKV2_000664"
     CDS             complement(67771..69957)
                     /gene="glgB"
                     /locus_tag="ACHKV2_000664"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO6938602.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(70230..71333)
                     /gene="asd"
                     /locus_tag="ACHKV2_000665"
     CDS             complement(70230..71333)
                     /gene="asd"
                     /locus_tag="ACHKV2_000665"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6938603.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            71525..72118
                     /gene="yhgN"
                     /locus_tag="ACHKV2_000666"
     CDS             71525..72118
                     /gene="yhgN"
                     /locus_tag="ACHKV2_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO6938604.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(72164..73459)
                     /locus_tag="ACHKV2_000667"
     CDS             complement(72164..73459)
                     /locus_tag="ACHKV2_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO6938605.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(73366..75490)
                     /locus_tag="ACHKV2_000668"
                     /pseudo
     CDS             complement(73366..75490)
                     /locus_tag="ACHKV2_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(75630..76970)
                     /gene="gntU"
                     /locus_tag="ACHKV2_000669"
     CDS             complement(75630..76970)
                     /gene="gntU"
                     /locus_tag="ACHKV2_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6938606.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76974..77501)
                     /gene="gntK"
                     /locus_tag="ACHKV2_000670"
     CDS             complement(76974..77501)
                     /gene="gntK"
                     /locus_tag="ACHKV2_000670"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6938607.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(77640..78635)
                     /gene="gntR"
                     /locus_tag="ACHKV2_000671"
     CDS             complement(77640..78635)
                     /gene="gntR"
                     /locus_tag="ACHKV2_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO6938608.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(78859..79554)
                     /gene="yhhW"
                     /locus_tag="ACHKV2_000672"
     CDS             complement(78859..79554)
                     /gene="yhhW"
                     /locus_tag="ACHKV2_000672"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO6938609.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(79677..80714)
                     /gene="yhhX"
                     /locus_tag="ACHKV2_000673"
     CDS             complement(79677..80714)
                     /gene="yhhX"
                     /locus_tag="ACHKV2_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6938610.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            81048..81536
                     /gene="yhhY"
                     /locus_tag="ACHKV2_000674"
     CDS             81048..81536
                     /gene="yhhY"
                     /locus_tag="ACHKV2_000674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6938611.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT        19675 a        22160 c        20462 g        19255 t
ORIGIN      
        1 tacataggga atgtaaatga acaaatttat taaagttgca ctggtaggtg cagtactggc
       61 tacgttaact gcatgtactg gtcatattga aaaccgtgat aagaactgct cttacgacta
      121 cctgctgcac ccagcaattt ctatttctaa aatcattggc ggttgcggtc ctactgcaca
      181 gtaagtctct gacagaaatc agctaacgct gatttaattg cctgacgccc tcgggattac
      241 ccgagggcgt atttgtttct atatccttct tgctccccac ccgtaaaaat attttcactt
      301 taatttcaat tggataacca cattcatcag ccacagcgcg atcctgtgcg aaattatgtg
      361 atcttcctcc acattacata acatcatatg ttgttatatt catcatgcat tgtcatgtca
      421 ccttttaaat gactgcaaac tctcccctac aacgtgttgg acaagaaaaa ggtatcgcta
      481 tgggaagcca ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg
      541 ggacaaccgt cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcggcgggca
      601 cgccgtggct gacggtgctc gcatttgtca tcggcgggtt aattgtgatc ccgcaaatgt
      661 gcgtatatgc ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga
      721 aaaatgccgg aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg
      781 atgcgccgtc attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc
      841 ctatcgatcc gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgctgc
      901 tgcacctgcg ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa
      961 ttatcccgtt cactatcgtc attggtcttg ggatcttctg gtttaaagcg gagaattttg
     1021 ccgcccctgc taccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga
     1081 tctctgccac cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa
     1141 ttaaaaaccc cggcaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg
     1201 tgctctacac cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca
     1261 attctgaaac gccgatttcc gacgccctga cctggatccc cgcactcggc agcaccgctg
     1321 ggatctttgt tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga
     1381 tgtaccagcc gcggctggaa tacgcgatgg cgaaagacaa cctgttcttc aaatgcttcg
     1441 gccatgtgca tccgaaatac aacacgccgg atgtctccat cattttgcaa ggggcgctgg
     1501 ggatcttctt catcttcgtt tccgatctca ccagcctgct gggctatttc accctggtaa
     1561 tgtgtttcaa aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt
     1621 acaaaccgct gtggcgtact ccggctttcg ggctgatgac ccccctcgcc attgcgtcaa
     1681 gcctcattct ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg
     1741 tcatcgttat tgccactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc
     1801 tcaacgcttt gtcgtaatgt tatctggaga aaataaaatg ttggatattg ataaaagcac
     1861 tgtggacttt ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga
     1921 cgttccgctg gtgcacacca tcgtggaaga gatggtgaag cgcgacatcg atcgtattta
     1981 tttcgttgcc tgcggatcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcagatcg
     2041 cttttccgat cttcaggttt acgccatttc cggctgggag ttctgcgata acaccccata
     2101 tcgcctcgac gctcgttgcg cggtgattgg cgtttctgac tacggtaaaa ccgaagaggt
     2161 aatcaaagcg ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg
     2221 cgcggatagc ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat
     2281 ctgggaaatt catctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc
     2341 gccgcacgcg gaaatcggca agatcaaaaa cgatctcaaa cagctgccga atgcgctcgg
     2401 ttatctggta cgcacttggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg
     2461 gccgatgatt tacacggttg ctgcaggccc gctgcgcccg ctgggttaca aagaaggcat
     2521 cgtgacgctg atggaattta cctggacgca cggctgtgtg attgagagcg gtgagttccg
     2581 tcacggcccg ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga
     2641 tgaaagtcgc cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt
     2701 gatcgtcatc gattacgccg caatttcgca aggactgcac ccgtggctgg caccgttcct
     2761 gatgttcgtg ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc
     2821 ggatgaacgc cgctattacg gtggtctggt ggaatattaa tcctctctcc cggcccggca
     2881 acgggccgga tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac
     2941 cagcggctgc ctattgaaca tgcattccgt gatgcaagcg agctgggtta cgatggcatc
     3001 gaaatttggg gcggccgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa
     3061 caaatcaagg cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc
     3121 aacggctatc cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac
     3181 atgatcaagc tggcgatgga tatggcaaaa gagatgaacg cgggttatac gctgatttct
     3241 gcggcccacg cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac
     3301 ttaagcgaac tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta
     3361 acgccgtatg aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg
     3421 gtgccttcgc cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa
     3481 ccggtgatga gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac
     3541 agcgacgggg ccagcgacac gcattacatt cccggcgaag gcaaaatgcc gctgcgggaa
     3601 ctgatgcgcg atattattga tcggggctat gagggatact gcacagtgga actggtaacg
     3661 atgtatatga acgagcccag actttacgcc cgccaggcgc tggaacgctt tcgcgcgctg
     3721 ctgccggagg atgagtgatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct
     3781 acccgcaact gaataaagcg ttttctggcg gtaatgcggt gaacgtggcg gtgtactgca
     3841 ctcgctacgg tataaagccg ggatgcatta cctgggttgg tgacgatgac tacggcacaa
     3901 agcttaagca ggatctcgcc agcatgggcg tcgatatcag ccatgtccat acgaaacacg
     3961 gcgttaccgc acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca
     4021 ccgaaggcgt gatggccgac tttgccctga gtgaagagga ttacgcctag ctggcgcagt
     4081 atgacattgt gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg
     4141 ctgcgggcaa acttaccgct ttcgacgtct ccgacaagtg ggacagcccg ctctggcaga
     4201 cactggtgcc gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc
     4261 gtctgaagat gaaagcgatt gttgcccgtg gcgcaggcgt ggtgattgtc acgttgggtg
     4321 aaaacggcag tattgcctgg gatggcgtgc agttctggcg tcaggctcct gaacaggtga
     4381 cggttatcga caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt
     4441 ctgcggggat gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcacaaacta
     4501 ttcagtacca cggtgcctgg taggcataac gttggcgtga gcatcttcac gccaacgtgc
     4561 tgttacttgc cggaaaacga ccctataatc cgtgtaattc attctttatt tcagggtcga
     4621 ttatgtcagc tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc
     4681 tgctggatga tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaatg
     4741 agctttgtac acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag
     4801 tggcagacgg cgtactgatc cgctggcagg gcaaaggcac ctttgtacaa agccagaagg
     4861 tcgaaaacgc cctgctgacc gtcagtggtt ttaccgattt tggcgtctca caaggcaagg
     4921 cgacgaaaga gaaagtgatc gagcaagaac ggatcagcgc cgcgccgttt tgcgaaaagc
     4981 tcaatatccc cggtaacagc gaagtgttcc atctttgccg ggtgatgtat ctcgataaag
     5041 agccgctatt tattgatagt tcgtggatcc cgctgtcgcg ttatcctgac tttgatgaga
     5101 tttacgtcga aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg
     5161 tcagcgacaa aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga
     5221 aatgcgaact gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca
     5281 aaccggtgca cgtctccgaa ctcttctgcc gcgccaaccg catcacctta acaattgata
     5341 ataaaagaca ttaaccgtag gccggataag atgcaccagc atcgcatccg gcgatgccgg
     5401 cgcactgaat tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga
     5461 ggggaccgtc cgagcgtttt gatattacgg cttcgccaca aaaccaatcg cttcgtacac
     5521 cgcttttagc gtacgggaag cgtgcgcgct ggctttttct gcgccgtctt tcatcacctg
     5581 ttgcaggaag gcttcatcgt tgcggaaacg gtggtaacgt tcctgcaatt cagtcagcat
     5641 accggaaacg gcatcagcca cttcgccttt cagatgacca tacatcttgc cttcgaactg
     5701 tttttccagt tccgggatgc tctggcccgt taccgcagaa aggatatcca gcaggttgga
     5761 aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg agtcggtgac
     5821 cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccaa taacgttatt
     5881 gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca ttacgcgcgc
     5941 gccggatttc ggaataaacg gctccggcac cttaaagata tcgccgtaca gcgcgttgaa
     6001 acgctgggcg atatcacggc tcagttccag gtgctgtttc tggtcttcac ccaccggtac
     6061 cagattagtt tgatacagca ggatgtccgc tgccatcagc accggatagt caaacagacc
     6121 agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgcgtca tgcggctcag
     6181 ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt ccggtacgtg
     6241 ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat acaaggccag
     6301 cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga tcgcgtgttg
     6361 gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact gacgcagcgc
     6421 acccatgtag ttaccaatag tcaattcacc tgagggctgt gcgccactaa aaacgatggg
     6481 cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg ggcagaaggt
     6541 catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg ccgtagttat
     6601 atccgtaggt taagccaact gatgggcaac ctgccgcttt tgctgcctga atatcattgc
     6661 gtgagtcgcc aacaaacagc atctgttgtg gagcaattcc catccgctca gccaccagta
     6721 acagcgggtc cggatgcggt tttttgtttt gcacatcatc accaccaatc accacgctga
     6781 agtatttggc gatatctaag gcttcgagca gcggcgcgac gaacggcgtc ggtttgttgg
     6841 tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg gcaacgtgcg
     6901 ggaacaaaaa cgtcccctct tcggcaacct cgccatagta acgatcgaac agtttacgca
     6961 gaatacgtac ctgttcttct gccggaatgt cgtcatcaac gggcggttta cccattgttt
     7021 tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc agaacatctg
     7081 cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacgggcaac tccagcgcat
     7141 acagcgccat atctaccgca gcagcaagac cgggagcact gtccaccagc gtaccgtcga
     7201 gatcaaaagc gacgccgcga atatcttcaa acttactcat gacttacctt tgccagttca
     7261 ctgcgcattt catcaatgac ttttttgtag tctggctggt cgaagattgc cgaaccggca
     7321 acgaacatat ccgcgcccgc cgcggcgatt tcgccaatgt tgttcacctt cacgccaccg
     7381 tccacttcca gacgaatgtc aaagccagac tcgtcgatac gacgacgtac ttcgcgcagt
     7441 ttatccagtg tttgaggaat gaaagattga ccgccgaaac cagggttgac ggacatcagc
     7501 aggatcacat ccagcttatc catcacgtaa tccagatagc tcagaggtgt cgccgggtta
     7561 aataccagac ccgctttaca gccattttct ttaatcagtt gcagcgtgcg gtcaacatgc
     7621 tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa atcaggcaca
     7681 atgcgatcga ccggtttcac catcaggtgt acgtcgatag gggcggtaat gccgtagtta
     7741 cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacgtagtg gttatccatg
     7801 acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc ttcacccagg
     7861 cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat ccgcttctcc
     7921 ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc agcagttcgt
     7981 ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg acgacatgca
     8041 attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag atcagcacag
     8101 gaatatggcg ctcaaccaga ccttcggcga tctccgccag atgcgcttgt tgttcaagcg
     8161 taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt gcataaggtg
     8221 gatcgcaata gacgacagat gcatcatctg cgcgcgccat gctatcggcg taagactcac
     8281 aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct tccgggaaat
     8341 agggtttttt gtagcggcca aacggcacat taaactcacc gcgcagattg taacgacaca
     8401 ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg aacggatcct
     8461 ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt gtttcgggaa
     8521 caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca atgttataga
     8581 gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg gtgttgagaa
     8641 acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc aaatgccgtt
     8701 taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg cgatttttct
     8761 tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga cagcatccag
     8821 cgcttcaggc aactcccgca acgataaacc attgcgggag ataaccttga ttacttcaga
     8881 tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctgaac atctgctggc
     8941 aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga aaccaggaca
     9001 taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag attctctttc
     9061 ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag agtgtaatgg
     9121 ctggacggtg ccgatttcaa cgaacccaca ttacctgcgg tcttccctcc agcggctggt
     9181 gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc cgtctctttt
     9241 ggtgctggcg tagaagtcgc agccggtgcc ttcgtgctcg ccacaggagc agctggttca
     9301 gtacgcttcg gcgtctgtgc taccggcttc ggttccgttt tcacggttgc ttgcggtttt
     9361 ttcggttcaa tcaccgcctg ctgacgagct ggacgcgtag tggccggacg ttcagcagtt
     9421 tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgta ctggcgcgac agttgctggt
     9481 tcagtcggca atgtagaatt gaccgccaca ttgttcaact gttgctgatt ttgtggctgg
     9541 gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc cgttgccgcc
     9601 ggggtttgcc cttgagtcgg cgtagaagag atcggtggca gagaaacatc ctgctgagta
     9661 ttttccgcag acgtggttcc cggcgctggc tgcacgccat tcgcctgatc ggtcgcattg
     9721 ccagcaagat caatactctt ctcgccagac gcggtttgat cgctggaagt ggtcgagggg
     9781 gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat gccgacgccc
     9841 atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt acgcggacga
     9901 cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc ttcttcgatt
     9961 tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc agtgtcatca
    10021 agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc agaacgttca
    10081 gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag ctcgtcttct
    10141 ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac gttgcaatta
    10201 acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt acgctgattg
    10261 acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa cttcactctt
    10321 accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct tgtcacgcag
    10381 catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg gtaacccagc
    10441 ccgcgtgagc agagttataa tacgctgcgt ttcggcagaa ctaaactgcc cgagacgttc
    10501 cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta accaattgcc
    10561 ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca gtaaagcacg
    10621 taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac aacaacggcg
    10681 aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat tctcttccag
    10741 ccagttaaaa aacgtaccgt caagaataat gccgtatttg atgacttctg ccagccccga
    10801 cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca ctgaagcagg
    10861 ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag ttttgccgcc
    10921 aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac gaacaccgcg
    10981 ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc caagcgccac
    11041 cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg tatcgagtac
    11101 agccaggctt ttatactgct cgccgtcagg aaggataacg ctatcgacgt taacacccgc
    11161 ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt cgttggtgac
    11221 caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat taaacaaacc
    11281 agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc tctccataac
    11341 gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca gaattagttg
    11401 ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc atcagtacga
    11461 atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa cgcttccaga
    11521 acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg ctgcgtgcgt
    11581 gcaagttgct tttcgatcgt cgtttcaaga taaacgacaa cgccacgagc ggaaagacgg
    11641 ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat accctgtttc
    11701 tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc gccttctaaa
    11761 tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc ggaatcgtaa
    11821 aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc ggcacccata
    11881 ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact aagactattc
    11941 gttaatgata aacccgcttc gctcagagag ctccgcagga catgaactga aacctcataa
    12001 gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg gcaactgaat
    12061 aaatcaccca tccctctgca tatctggtcg ctgcaagcgc gctgccttgc taccaccgct
    12121 ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc cgcctgaatg
    12181 ataaatcatc atcatggaag gcaggcagcg aagagcacca actctcaaat cggtactcct
    12241 tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcgta aaaacattac
    12301 cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca ccactaactc
    12361 gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg gaatgtcgcc
    12421 aagcaacggc acgctatcct gacccgattt atttttacgg gtaaaaatgc cgcccagcgc
    12481 caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat caatcgccag
    12541 cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga tgtgtaattt
    12601 cagccggatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca ggacggcctc
    12661 tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg gaatttcgct
    12721 cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac tggcgataat
    12781 atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagcaaac gtccgttgat
    12841 gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt cgctaccaag
    12901 cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc atttcacgcc
    12961 taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct caacctgccc
    13021 gaccggcaga tccatttgcg ctacccactg ttcaagggcg cttaacgccg ttttgttatc
    13081 tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg cactcagtag
    13141 cttctcccca gctttcgcca gctctccggc gtcggcgtat tgcagagtaa tattgcgatt
    13201 ttccagcggc agatttgcct gcgcccgcgc ctgctccgct tcctgacggg cgatattgtc
    13261 attctgccag gcaatggaat gcactgagag aatgttgccc tcctggcgcg ttatcagtcc
    13321 ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta gatgtaacga
    13381 caccgtaccg ctgacgtctg gcgacaccac caggttcaac ttctcctgtt cagccagcgc
    13441 ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt gcggctttgc
    13501 cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct tcatttgtat
    13561 ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggta cccagcgtta
    13621 gtacgtctag cgtcagctgt aaaattgtcc agccgttttc cagcacatcg ttttgctgca
    13681 cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgcgctcg cctcgcccta
    13741 ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct tccggcggtt
    13801 taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac aaccagcgtt
    13861 taaccctcat gcggcgtctc cagttgtagg gtgaacaaaa gatcatcacc ttccacgctt
    13921 aatgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc cgacggcact
    13981 gcttcccaca gcgttttcaa cgccaactcg cctccctgcg cggatggatg ccagaaaacc
    14041 agttgtgcgc cgggcaactg aaaatccagt ggcgaaaagg gcagcgtttt ttcttcgctg
    14101 aagggagtgg tgtcaaccag gcgatacagt gtggcccaat gctgatgatg acttgcccgt
    14161 agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg acagaaaaat gagcgtaact
    14221 aacatcagca accagaatgc ccagcaaaac tggcggaggc ggggtgatgt ggcgaaccac
    14281 cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa attgccagcg
    14341 tccctgcgca tcctgctgcg tggccccccg ctgattgaga tgaaaagatg catcctggcg
    14401 gagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt gtaagcccct tgatctccag
    14461 cgttccctgc tgccagctta tcgttgtcag ccaggcgtgc tcgggtaaaa gcgccgccag
    14521 tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc tttgcaaacg
    14581 ctgctcacga atttgttggc gctccagcaa acgtggtttg gttatctgta agctgcgggc
    14641 gagttgttgt tccccctgaa gcaaaacggc gtctacgcga gcttcggcgc tgcttgtcag
    14701 acgcagtatt agcgttatcc cgacggccag cagcagaggc gcaacgaaca tcagcaacca
    14761 gaaacgaaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa ttggcgggtt
    14821 catcagtacg ccttcccaag cgccagccca gcgcgatggc aaagtctcca cctggcggcg
    14881 gtaacggcgg ctgacgaaca gaaacggcct cccaaggatc aaatccgcct tcgccacata
    14941 tcgcgacgct ttcgggatca acggataacg ctgccgccag ctccttcgcg ctagtcatcc
    15001 ccaccgccag tttgcgcccc cagcgatagc gcgtcgccca cagccactgt tcgttatcac
    15061 gccaggccag acattgctga tgagaaggta aaaaaggcag gaatcgctgt aatgcactgg
    15121 catccggggt gatcgcacta acatgaacac gcaacctttc tgccaaagta agcagcgttg
    15181 ccagctcttt gctttgcgcg gcagtcacgt tataggcggg actgagtgag tcttcgctgt
    15241 aatcgaagcg cagggagtcc ggatccatat ccagctcgcg agccatcgtc ccggacagcc
    15301 aggccgtttg ctcccgctca ccgagggaca tcgacgggcg cggaaatgac cgctgtaatg
    15361 tgcgactggc gggaaacgcc aacatgatgt gatggcgctg cggcagttcg cgactccacg
    15421 gtaacaacgt tttagccagc cgctgcgcat caacaatccg cccatctttg ataatgtcgt
    15481 gcgccagcgg caaccgccac cagcgttgca aaaagcattc ttttgcgccc cgcacaatag
    15541 caaccgctac cgcttcttgc tgttgtaaat gcaaaccaat ttgccagatc ttaaatgcca
    15601 ttgtgatgat ctccttatca cccgtcactc tgacgggtat atcaatgcgt ctggcttgcc
    15661 tttatactac cgcgcgtttg tttataaact gcccaaatga aactaaatgg gaaatttcca
    15721 gtgaagttcg taaagtattt tttgatcctt gcagtctgtt gcattctgct gggagcaggc
    15781 tcgatttatg gcctataccg ctacatcgag ccacaactgc cggatgtggc gacattaaaa
    15841 gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg atggcgagct gattgctcaa
    15901 tacggtgaga aacgtcgtat tccggttacg ttggatcaaa tcccgccgga gatggtgaaa
    15961 gcctttatcg cgacggaaga cagccgcttc tacgagcatc acggcgttga cccggtgggg
    16021 atcttccgtg cggcaagtgt ggcgctgttc tccggccacg cgtcacaagg ggcaagtacc
    16081 attacccagc agctggcgag aaacttcttc ctcagtccag aacgcacgct gatgcgtaag
    16141 attaaggaag tcttcctcgc gattcgcatt gaacagttgc tgacgaaaga cgagatcctc
    16201 gagctttatc tgaacaagat ttaccttggt taccgcgcct atggtgtcgg tgctgcggca
    16261 caagtctatt tcggaaaaac ggtcgaccaa ctgacgctga acgaaatggc ggtgatagcc
    16321 gggctgccga aagcgccttc caccttcaac ccgctctact cgatggatcg tgccgtcgcg
    16381 cggcgtaacg tcgtgctatc gcggatgctg gatgaaggat atatcaccca gcaacagttc
    16441 gatcagacac gcactgaggc gattaacgct aactatcacg cgccggagat tgctttttct
    16501 gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata accgttatgg cgaaagtgcc
    16561 tatgaagacg gttatcgcat ttacaccacc atcacccgca aagtgcagca ggccgcgcag
    16621 caggcggtac gtaataacgt gctggactac gacatgcgcc acggctatcg cggcccggca
    16681 aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca acaagattac cgatacgctg
    16741 aaggcgctgc cgacctatgg tccgctgctg cctgccgcag tcaccagcgc caatcctcag
    16801 gaagcgacgg caatgctggc ggacgggtcg accgtcgcat tgagtatgga aggcgttcgc
    16861 tgggcgcgtc cttaccgttc ggatactcag cagggaccga cgccgcgtaa agtgaccgat
    16921 gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg gcgatgcatg gtggctggca
    16981 caagtgccgg aagtgaactc ggcactggtg tcgatcaatc cgcaaaatgg tgccgttatg
    17041 gcgctggtcg gtggctttga tttcaatcag agcaagttta accgcgccac ccaggcactg
    17101 cgtcaggtgg gttccaacat caaaccgttc ctctacaccg cggcgatgga taaaggtctg
    17161 acgctggcaa gtatgttgaa cgatgtgcca atttctcgct gggacgcagg tgccggttct
    17221 gactggcagc cgaaaaactc accaccgcag tatgccggtc caattcgctt acgtcagggg
    17281 ctgggtcagt cgaaaaacgt ggtgatggta cgcgcaatgc gggcgatggg cgtcgactac
    17341 gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa acattgtcca caccgaatcg
    17401 ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg cgcgcggcta cgcggtcatg
    17461 gcgaacggcg gcttcctggt ggacccgtgg tttatcagca aaattgaaaa cgatcagggc
    17521 ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg aatgcgatat tccggtgatt
    17581 tacggtgata cgcagaaatc gaacgtgctg gaaaataacg atgttgaaga tgtcgctatc
    17641 tcccgcgagc agcagaatgt ttctgtacca atgccgcagc tggagcaggc aaatcaggcg
    17701 ttagtggcga agactggcgc gcaggagtac gcaccgcacg tcatcaacac tccgctggca
    17761 ttcctgatta agagtgcttt gaacaccaat atctttggtg agccaggctg gcagggtacc
    17821 ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg gcgggaaaac cggtaccact
    17881 aacagttcga aagatgcgtg gttctcgggt tacggaccgg gcgttgtgac ctcggtctgg
    17941 attggctttg atgatcaccg tcgtaatctc ggtcatacaa cggcttccgg agcgattaaa
    18001 gatcagattt caggttacga aggcggtgcc aagagtgccc agcctgcatg ggacgcttat
    18061 atgaaagccg ttcttgaagg tgtgccggag cagccgctga cgccgccgcc gggtattgtg
    18121 acggtgaata tcgatcgcag caccgggcag ttagctaatg gtggcaacag ccgcgaagag
    18181 tatttcatcg aaggtacgca gccgacacaa caggcggtgc acgaggtggg aacgaccatt
    18241 atcgataatg gcgaggcaca ggaattgttc tgattaaaaa ggcgcttcgg cgccttttca
    18301 gtttgctgac aaagtgcact tgtttatgcc ggatacgggt gaacgcgtta tccggctaac
    18361 aaaatcgtga aaactcaata tattgcagga accccataag cctgataagc attgtgcatc
    18421 aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg cacgaggaac
    18481 agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc
    18541 gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg
    18601 tagagatctt gcgctaccac gatattcatt ttgctggaaa agtaagacgg tgccatgctg
    18661 agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg
    18721 ttagcggctt cgaagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg
    18781 taggattcag ttcccactgc atattcgcgg atcaggatca ggtgatcgtc cacaatcggc
    18841 acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca
    18901 ttgctgaact ctagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca
    18961 acattcagaa tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat
    19021 cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca
    19081 tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata
    19141 actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt
    19201 gatgtgctta atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca
    19261 tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg
    19321 agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg
    19381 ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa
    19441 acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg
    19501 caggtattac aggtgcctgg cccaacggga gccagcgcgg caccgatctc tctggcgctg
    19561 aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacgcgtta cggcatctct
    19621 accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgccccct
    19681 ttctgggagc agtacatcaa cgatgagaat accgttgaac tgattcatac cgattcgctg
    19741 ccgctggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga aactcgcggc
    19801 tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa
    19861 atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag tcgtccgcgc
    19921 gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctgatt
    19981 accccggatg tatttgttcc gtggctggca ggcggcgcgt tactgctgct gggcgcaggt
    20041 ctgtgggggc tattcgcccc cccggcaaaa tcctccctgc gggaaattca ttgtctgcgc
    20101 ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt
    20161 tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat
    20221 ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acctagtgcg tcaggggcga
    20281 tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg
    20341 attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctggat cccgctggat
    20401 atgccgctga aattcactct ctcatggatg aaaggtgcgc agaccattga agccaccagc
    20461 gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg
    20521 ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta
    20581 ccgtttgact gctcgcagat catctggaac gacgcccgct cattaccatt accagaatct
    20641 gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa
    20701 ccggaagatg aatctcgcgt cagcgcctca ttacgttcag caattcaaaa atccggcatg
    20761 gtattgcttg atgattttgg cgacattgta ctgaagacag cggatctgtg ttctgccaaa
    20821 gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac
    20881 gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg
    20941 gtgagtgcgg aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat
    21001 gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc
    21061 agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac
    21121 ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg
    21181 tttaacgccg agggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatata
    21241 ggccttcatc cgatcccgga tcgttccggc ctgtggcgct atctcagcac cacgctgctg
    21301 ctactgacga tgctgggtag cgccatttat aatggcgtac aggcctggcg gcgttaccag
    21361 cgtcatcgca ctcgcatgat gaagattcag gcctattatg aaagctgcct gaacccgcaa
    21421 ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc
    21481 ctgtcgcgca aattgcttca ctctggagat tttcctcatg catatcaaca ttgcctggca
    21541 ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg ccttcgataa
    21601 ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg ttacgccaca
    21661 ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta
    21721 ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat
    21781 gggaccgcgt gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag
    21841 cggtaagcag cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttga
    21901 gcataccggt ctggacgcac accttgattt attactttcc acccacacat ttggttatcc
    21961 gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag
    22021 aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg
    22081 ttactgcctc ggcgtgacta atcctgactc agggattgcc gagaaacagt atcaacgcca
    22141 tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga
    22201 aagaaaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata
    22261 aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc
    22321 gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg
    22381 acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag
    22441 ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgctggcac
    22501 gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc
    22561 gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga
    22621 ttatgccgca acatgaccaa ttacatcgct atctgtttga aaactttgcc gtgcgcggcg
    22681 aactggtaac cgtttcggaa accctgcaac agatccttga gaatcacgat tatccgcagc
    22741 ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga
    22801 agtttgatgg tgatatcacc gtacagctgc aaggcgacgg tccgatgaat ctggcggtta
    22861 ttaacggtaa caacaaccag cagatgcgcg gtgtggcgcg cgtgcagggc gaaattccag
    22921 aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc attaccccga
    22981 gcgaaggtga acgctatcag ggcgtggtgg gtctggaagg tgataccctg gcggcctgcc
    23041 tggaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg
    23101 acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct gcgcaaaatg
    23161 cccagcagga cgactttgac cacctggcga cgctaaccga aaccatcaaa accgaagaac
    23221 tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg
    23281 tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg
    23341 cgctgaaaac gctgcctgat gaagaagtcg atagcatcct ggcggaagat ggcgaaattg
    23401 acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa
    23461 tccgcaacaa cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga
    23521 gagctctgcc ggattttacg actaatacac ctgatgcgct ttgtcactca ttcagattca
    23581 catccagcct ttcaccatcc actacaagat cgcgccacgc tttaggcttc gttcccagtt
    23641 ttccatcctt cgcagctgtg agtaattttt ctttcgttat atcacgcgga agtaaactca
    23701 tcgtaagctc ttgccattct ttcttgtcag gatcaaagcc atagacaatg tatctttcat
    23761 catcaaaggc aaagaggatc cgctctgccc tgccatcact attcagatct tgctcgacca
    23821 gtacgcaggc gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg
    23881 accagaatgt cgcatccggt ttaccagaac caggggcaat taacacattt tcggctaata
    23941 ctttttccga tatctgttgc tgaagatgtt gctctccatc taacgccatc agtagatccc
    24001 gcgcgcgttt cgggtctttc atatactcgg catcgctttt cagcgactca agcgccgcac
    24061 gtccatagcg accgctgtgt tcgagcatgt agatagttac ctggtctggc gtgtttttgc
    24121 cgctctgata acgcgccata tgactgttca cactaatgcg catactgtcc agcaccggcg
    24181 aattaagaag caccagtatt accaacacca ataacgaaac cgcaaggttc actttgccct
    24241 gaagaacaag gggattttgc cctttacgcc agacgatact gacaaaatac cccagcgacc
    24301 ataccagtaa caccagcacc gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact
    24361 gagcgacccg cagccataac gcccaggcgg cgatgaacac atacagcgga gcaaccagca
    24421 gagcggtttt aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat
    24481 cgcggacaat agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg
    24541 cggagatgtg gcgagaaatc gcactcaggc ctgtaaacgg cagggtgatg ataaacatca
    24601 gggttagcaa tgatactaac ggcagcaacc ccgtggcgat taacgtgaac aacttttgaa
    24661 tagagtcgat taaacgtgac tgtgttcgcg cgaggatcac cgccagcgcg gtaaccagac
    24721 ctaacgtgag ataaataaac cagtcggttg caaaaaagag cgttttgaaa aacgtgatgc
    24781 caacaagttt aaacaactca ctccacagta aaagcaccag ccacgtcaga ccattggcga
    24841 gaaaaatcac cagtaatata agtacgttat gccatactga ctggtaaaaa tagcgatagc
    24901 ggctactgtc attgcggatg cgaaggcttt gttgtatcca cggtagcaac aacattgcca
    24961 tcagcagcag atagcaacca aaatcccaaa gcgccttttc agctctccag ggggtcatgc
    25021 catcagtttg ccacttcagc cagccgctca tccccagcgt ggcaataaac accagcgcca
    25081 gccatcccca aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa
    25141 acgcgacggt cgcaggcacg ccataaacaa tccaactgtg attttttccg gaaagccagg
    25201 cgatcagcag gtagcaaacc agaccctgaa gtaatccggt agcaatcatc ccccagcgtg
    25261 ttgcgggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat
    25321 tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt
    25381 ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat gcgattccac
    25441 tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta
    25501 aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc
    25561 aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa
    25621 aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa
    25681 tggtttgacc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta
    25741 caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga
    25801 gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg
    25861 ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc
    25921 agacaaaggc aaaggtaaga acgataacaa acctctctct ccggaaacct ggcaacatct
    25981 gaaaggcctg gtgaccaaac agctttccgg caaacgtctg ttcgttgtcg acgccttctg
    26041 tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc
    26101 gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagttggcag gtttcaaacc
    26161 agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct
    26221 gaattccgaa aacttcgtgg cttttaacct gaccgagcgc atgcagctga ttggcggcac
    26281 ctggtatggc ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct
    26341 gaaaggtatc gcttctatgc actgctccgc caacgttagc gagaaaggcg atgttgcggt
    26401 gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga aacgtcgcct
    26461 gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaacttcg aaggcggctg
    26521 ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg
    26581 tcgcgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga
    26641 tggttcaaaa accgagaaca cccgcgtttc ttatccgatc tatcacatcg ataacatcgt
    26701 taagccggtt tcaaaagcgg gtcacgcaac gaaagttatc ttcctgaccg cagacgcctt
    26761 tggtgtgctg ccaccggttt ctcgtctgac tgccgatcaa acccagtacc acttcctctc
    26821 tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga cgccaacctt
    26881 ctccgcttgc ttcggcgcgg cattcctgtc gctgcacccg actcagtacg cagaagtgct
    26941 ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg gctggaacgg
    27001 cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg
    27061 ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga tcccaaccga
    27121 actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt ccccggaaca
    27181 gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaactttg ataaatacac
    27241 cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat gatttgaagc
    27301 tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt
    27361 ttatttaccc ttcttttgtc gtgctctgcg cccgcgttac cggcacaggc agccaggcgc
    27421 gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta tggttatcca
    27481 cgatacgctg cacaatcgcc agccctaatc ccgtgccgct aatggtgcgt gcactgtcgc
    27541 cgcgaacaaa cggctggaaa aggtgcttac gttgttccgg cgcaattccc ggaccgtcat
    27601 cttccacctg gaaccaggcg cgattcggct ccgtcccgct gctgactttg atccagccat
    27661 tgccgtaacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc gacagcgggt
    27721 gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc tcatagccac
    27781 tttcggcagc aataacctca ccgagtactg cgttgagatc cgccatttcc atcggcatct
    27841 cctgtccggt gcgcaggtag tcgataaact gctcaatgat ggcattgcac tcctcgatat
    27901 ctttattgat cgattctgcc aggtagccat cctgctcgct catcatctcg gtcgccaggc
    27961 gaatacgcgt cagcggtgta cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt
    28021 catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcgcgggta acggaacgca
    28081 cctcggacgc gccatactca cgcagcggcg gcggaataat ccctttacca acctgcaagg
    28141 ctgcgtgttc gagatcgacc aacggtcgat tctggatacg aataaacagc cacgccccgc
    28201 ctatcgccaa tagcataatc gccaacgtat agcggaacag cggagagaaa tcgccctgat
    28261 gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt ttcagccaga
    28321 cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc agttgctgtg
    28381 ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag
    28441 cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag
    28501 gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt
    28561 cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca
    28621 ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg
    28681 caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa
    28741 gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat cttcttccac
    28801 catgcggcgc agacgagaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg
    28861 accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag
    28921 tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcgtctt cgcggaacat
    28981 ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga
    29041 cggtgcgcct ggtagctcgt tcgcctgacg acgcagcacc gcacggatac gggccagcag
    29101 ttcacgcggg ttaaacggtt ttggaatgta atcgtcagcg ccaatctcca ggccaacgat
    29161 acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt tgctctgact
    29221 acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat ccagtaccat
    29281 aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg
    29341 aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc
    29401 atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag gttcgcaaca
    29461 atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa
    29521 tttcagccta aattgttaca aagcatatta aacagcagct taagtataca atttattcgg
    29581 cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac
    29641 agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac
    29701 tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga
    29761 cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc
    29821 gcctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcatggaa aatctcaaaa
    29881 tcgtcgatta ctccccgcag caggaaggca aagtcttttt tggcgcgtgg gtggagattg
    29941 aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc
    30001 gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg
    30061 gcgatctggc tgtggtgaat actcctgccg gggaagcgag ctggtatgtt aatgctatcg
    30121 agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag
    30181 ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga
    30241 atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa caggttgacg
    30301 ctgccgttcg cctgcttgac gaagggaata ccgtgccttt tattgcacgt tatcgtaagg
    30361 aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgggatacc
    30421 tgcgcgagct ggaagagaga cgtcaggcga tcctcaagtc catttccgag caaggcaaac
    30481 tcaccgatga tctggcgaat gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc
    30541 tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt gaagcagggc
    30601 ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg
    30661 cgcaatatat tgatgccgat aaaggcgtgg cagataccaa agccgcgctg gacggcgcgc
    30721 gctatatcct gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt
    30781 atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa gaggaagggg
    30841 cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg
    30901 cgctggcgat gttccgtggg cgtaacgaag gcgtacttca gctttcgctg aatgccgatc
    30961 cacagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg gatcaccttg
    31021 gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aggcgtggtg agctggacgt
    31081 ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggcaccgtg cgcgaacgtg
    31141 cggaaaatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggctgccc
    31201 ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg
    31261 cggtggtcga tgccactggc aaactggtag cgaccgatac catttacccg cacaccggac
    31321 aagccgcaaa agcagcgatg actgttgctg cgctgtgtga aaagcataac gttgaactgg
    31381 tggcgatcgg caacggtaca gcttcccgcg aaaccgaacg tttctatctc gacgtgcaga
    31441 agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt
    31501 actcggcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg ctgcgtggcg
    31561 cggtttctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga
    31621 aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg gcccgcaaac
    31681 tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg
    31741 ttccgctatt aactcgcgtg gcgggcctga cgcgaatgat ggcgcaaaac atcgtggcct
    31801 ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc agccgtctgg
    31861 ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt gacaacccgc
    31921 tggacgcctc taccgttcac ccggaagcct atccggtggt ggaacgcatt ctggcagcaa
    31981 cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcgt
    32041 ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg
    32101 aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg
    32161 agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgctgtcacc aacgtcacca
    32221 actttggcgc gtttgtcgat atcggcgtgc atcaggacgg cctggttcac atctcttcat
    32281 tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg
    32341 tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg cgtctggacg
    32401 agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa cgatcgcccg caaaacaacc
    32461 gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaacagc gcgatgatgg
    32521 acgcgctggc ggcagcaatg ggcaaaaaac gttaaacgcc cggactggcc cctaaacctg
    32581 cgcgataagg cgttcacgcc gcatctggca acgaacgcct tgcctgacat aaaagtgccg
    32641 gagaatatct ccggcatatt tattccacag ccaaactcac aatatatttc gttaatattt
    32701 atcacttcat taacaactga aaccttaatt aaacattagc cagtcctggt aattcgctat
    32761 tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag tcagattatt
    32821 attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt ctcattatca
    32881 ttgtactatt gattatttaa tctctccttc gttggcaaat catctggtct catgtcgctg
    32941 tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata
    33001 cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc cggcatatcg
    33061 ccaaaaactg ctttctcttg gcatgttacc tggctcctct tttaatgtgg tgcgcgtcgc
    33121 tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa
    33181 agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga
    33241 aaaaattaac cataggctta attggtaatc caaattctgg caagacaacc ttatttaacc
    33301 agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag
    33361 aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt
    33421 ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt
    33481 tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc
    33541 tgtacctgac gctacaactg ctggaactcg gcattccctg catcgtggca ctgaacatgc
    33601 tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctgtcg gcgcgtcttg
    33661 gctgcccagt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg
    33721 cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca cagccgctac
    33781 tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc
    33841 gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag
    33901 cgtcgcagca tcttgatgcc gccctcgccc gtctgcgtaa tgagatggac gatccggcgc
    33961 tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca
    34021 ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt
    34081 tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg gctatcaaca
    34141 tcggcggggc gttacagccg ctgtttgacg tcggctccgt ggctctattt gtgcatggta
    34201 ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccagg
    34261 gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc atgatgtacc
    34321 tgttcctctc cttccttgag gactccggct atatggcgcg tgcggcgttt gtgatggacc
    34381 gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc gtcggtttcg
    34441 gttgtaacgt accgtcggta atgggtgcac gtacgcttga tgcaccgcgt gaacgtctga
    34501 tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat
    34561 tcgcggctgc cttcttcggg cagaacggtg cgctggcggt cttctcgctg tatatgctcg
    34621 gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag
    34681 cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa agcctgatta
    34741 tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc tggtaaagtg attatcatcg
    34801 tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc gtcgataaca
    34861 tcaacgactc ggcgctggct tccgtcagcc gggtgatcac cccggtcttc aagccgattg
    34921 gcgtgcatga agataactgg caggcaacag ttggcctgtt tacgggtgca atggcgaaag
    34981 aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt
    35041 tcaatccggc ggaatttaac ctcggtgaag agttgttcag cgcggtagat gaaacctggc
    35101 agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa gctagcaaag
    35161 gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag
    35221 ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc tcggtgatgg
    35281 gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga
    35341 atatcgctta ctcactggca acattgttct atcaagtcgc cagctacagt cagcacccaa
    35401 cttacagcct ggtgtgcatt ctggcggtta tcctgtttaa catcgtggtt atcggtctgc
    35461 tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag tcggtaagta
    35521 gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta ttcaggtgcg
    35581 tgatttgctg gcgttacggg gccgtatgga agcggcccag ataagccaga cattgaacac
    35641 tccacagcca atgattaacg ccatgctgca acaactggaa agtatgggca aagccgtgcg
    35701 gattcaggaa gaacctgacg gctgcctctc cggcagttgt aaaagctgcc cggaaggaaa
    35761 agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa
    35821 gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa aatgcaattc
    35881 ttgcagcgcg acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact ggctaatgca
    35941 caggtgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg aacgccatga
    36001 gcaaaaagca gagttccacc ccacacgatg cgctgttcaa actcttttta cgccaaccgg
    36061 agacggcacg cgattttctt gcgtttcatt taccagcacc cattcacgcg ctctgtgata
    36121 tgaaaaccct caagctggag tcgagcagct ttattgatga cgatctgcgt gaaagctatt
    36181 ccgatgtgct gtggtcggtg aaaacagaac aaggaccagg atacatctat tgtctgattg
    36241 aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac gctattgccg
    36301 caatgcaaaa tcaccttgat gccggataca aaacgttgcc gatggtggtg ccattgttgt
    36361 tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat tgtttcgccg
    36421 atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat atcaccgtca
    36481 tgcctgatga tgaaatcatg cagcaccgac gcatgacgct gctggagtta attcaaaaac
    36541 atattcgtca acgcgacctg atggggctgg tagagcaaat ggcctgctta ttaagtagtg
    36601 gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa accggcgatg
    36661 cggtacgttt taacgatttt atcgacggcg ttgccgaacg atcaccgaaa cacaaggaga
    36721 gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa gccctgcata
    36781 tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcgc tttaccgggc
    36841 tgtcagaaga agagttggct gcggcgagtc agtaaagttc tgtctcgcca ttcaaaagtg
    36901 cctacaccct ctgcttcaac gccaccagca cgtgacgaaa ctcgaccgga tgcgaaataa
    36961 acggcgcatg agccgctttg gcgaagatgt atgactcgct gtgtggccag agtttatcca
    37021 gcatcggcac cactttgcgc ggcaccagac catcgagata gccatacagt cgcaaaaacg
    37081 gcatgggtac gttttgcagc ggcaagcgga gatcaaccgt tttgaggatt tccagcccac
    37141 cattgagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
    37201 catcctgacg cgccgtttca gtacccatgg tttgtaacgc aaggaaccgt tccaccgtac
    37261 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
    37321 gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
    37381 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
    37441 aaatggcttt atcaggtgcc tgtcgcagca cggcttcggc catctccgca agtgacagcg
    37501 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aagatgcagc gtaaaatgcg
    37561 agctaagttc ctcgtcaatg caacgccaca cttcagcatt cagtccccat ccgtgcagca
    37621 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
    37681 gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta tgctggctat
    37741 gccgaatgcc actggcatta ggtcattggg ggatttgttc ggtctgctca cgcgccgccc
    37801 gtacagataa aacgttatgc ccacaatgtg gattacccgc cacacactcc catcttccct
    37861 gcggtcgctg cctgcaaaaa ccgccgccct ggcaaagact ggtcacggtt gccgactatg
    37921 cgccgccgtt aagtctgctc atccaccagc ttaagttttc ccggcgcagc gaaatcgcca
    37981 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc
    38041 aattgccgga tcgtatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat
    38101 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgcactgc caatgggata
    38161 gcgaagccat cacacgtaca cgggccactg cgacccagca ttttcttagc gcccggctac
    38221 gcaggcgcaa cctgaaaaat gcctttcgcc ttgaattgcc tgtgcaaggt cgccatatgg
    38281 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
    38341 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
    38401 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
    38461 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga agaagggaca
    38521 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat
    38581 tgtccgccgg atgccgtgga agccaccgac accgccctga aatttgacct gctgaccgcg
    38641 tatgttgatg agttaagcgc accatacctg gaagatgcag agatcgattt tgttaccgac
    38701 cagttgggtt ctcagctaac actgaaagcg cctaacgcca aaatgcgtaa agtggcagac
    38761 gatgcaccac tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt
    38821 gctggtcacg gtggccgcgt ttcactgatg gaaatcaccg aagacggtta cgccattctg
    38881 caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc
    38941 gagaagcagc tactaaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac
    39001 cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga
    39061 cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggcg cagcattgct
    39121 ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat
    39181 ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca cgtgggtacc
    39241 aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc
    39301 gcaatatctc tgttcccgat tgggataatt agagcttgtc gtcagaaaat tgacgttacc
    39361 cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc
    39421 ttgttattac tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg
    39481 gcgcttgcgg ttggattaat gcaagggatg ccgctggata aagttattgg ctccatcaaa
    39541 gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg
    39601 ggcaaaatgc tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa
    39661 tttggtaaaa aacacatcca gtgggcggtg gtactgaccg gttttaccgt tggttttgcc
    39721 ctgttctatg aagtgggctt tgtgctgatg ctgccgctgg tgtttaccat cgcggcttct
    39781 gcgaatattc cactgctgta tgttggtgta ccaatggcag ctgcactgtc tgtgacccac
    39841 ggcttcctgc caccgcatcc gggtccgact gcgattgcca ccattttcaa tgccgatatg
    39901 ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg
    39961 gtttacgctc gcgtgttgaa aggtatcgat aagccaattc cggaaggtct ctacagcgcg
    40021 aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg
    40081 ccggtagtac tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct
    40141 ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg ctacgctgat tgccgtgctg
    40201 attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg
    40261 ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg
    40321 ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa
    40381 accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg
    40441 ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg
    40501 acgggtgtta gcccggagct gatggttatt gcggtgggtt ccggtagtgt gattttctct
    40561 catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag
    40621 accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt
    40681 ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca
    40741 accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac
    40801 ttgttcacgc catcatcggc aaacatcgtc tcaagcgttg cggaaagctt gcgtcgccag
    40861 tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct
    40921 tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacga
    40981 ttaagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
    41041 ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga
    41101 ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg
    41161 ctctcccagt agccgcgtag cgtaggcagg tcatgtgtcg ccgcaaccgc catcgactgc
    41221 tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact
    41281 ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
    41341 agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc
    41401 agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc
    41461 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
    41521 ttggcacgca acagctcgat aaacggttca taagcacgcg cggtgatgat atgcgggtcc
    41581 attggcggca atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc
    41641 gacgctttca ggcaatacag ttcacggtcg caccaggttt ccgccccacc ttccgctacg
    41701 ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag
    41761 caggcggcaa actggctgta agcctgccac tgcaaccaga gataaaaatc aacgtcatcg
    41821 cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttctgata catctctggc
    41881 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca
    41941 aaggctgcct gccagaacag gctgtcgcct tgatctgcaa caaactggcg aaacgcggcc
    42001 atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    42061 agggcggtaa ccgtggagta atcgacccag tcggcatccc gcgcctgttg cagcgtctgt
    42121 tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcgacg
    42181 gcgttaacgt cgatataaat cacattcagc cagcggcgag aagacgggct atacgggctg
    42241 gcgctctccg gatttgccgg atagagcgca tgaatcgggt tcagaccaat gaacgatccg
    42301 ccacgttttg ccacatccac tagcatcgct ttgagatcgc caaaatcccc aataccccag
    42361 tttttttccg atcgcagcgt ataaagctgg acgcaggcac cccacagctt ttgtttattc
    42421 agcaacgcct gcggttcgta acagcgtttc ggggcgacaa tcaccctgca atgcccgcgc
    42481 tggtcttcct gggtaagcgt cagcgtgtga taaccttccg gcagcttcgt cggtagattg
    42541 aacgctttgc cccccgttat atggcctttg tactgcgttc cttcttcggt ggtcagcagc
    42601 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    42661 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    42721 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa
    42781 tgagggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt
    42841 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    42901 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    42961 tacagcacat ccacctgctt ttgtgcctct acataggctg cgaaatccgc catcaccaga
    43021 tacggatcgc cgccctgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    43081 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    43141 ttccgccatt tcaccgggtc gtagcctttg gccagaattg ccttcacttg ttccacggta
    43201 tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcccca
    43261 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    43321 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    43381 gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagcgga
    43441 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattttt cgccaggtag
    43501 tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgtggg
    43561 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg
    43621 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg
    43681 gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat
    43741 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg
    43801 ttagcccact cttttttcag tgatttatcc aacagagccg ccagtgccgg gttgcactgt
    43861 ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc
    43921 tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg
    43981 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt gtcgtgcacc
    44041 accgccagtt tagcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta
    44101 aagcgagtat taatttcgtt aataatctgc atgtggcgcg gcagtaagcc tttcaccagt
    44161 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    44221 ttgctggtaa tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    44281 aacagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac ttcgtaatcc
    44341 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa
    44401 caggcacact ggaagtactg ctgcatcaag cgcagctttt taccggcagt atggttgtcg
    44461 tttggataga gaactttggt cagtttttcc gcattgatgc cctgctgttc ggcacgcagg
    44521 aaatcaccgt cgttaaattt agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga
    44581 cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    44641 gtaatggtaa actccggctc ccagcgtccg tctttcgtca ctttaccgcc aatccctacc
    44701 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc
    44761 ggcgcttcaa cctgtttgcc atccacaaaa gactggcgga acaaaccata ttgatagttc
    44821 agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg aatcgaggaa gcacgccgcc
    44881 agacgtccca gaccaccgtt acccagcgca gggtcgatct cttcttccag caggtcagtc
    44941 agattgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    45001 aggttgttgc ccgtcagacg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    45061 tgattcgcca ccggcttggc gaatggctga gcacgcagca tttcggccag tgcttcactc
    45121 actgccagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    45181 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    45241 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    45301 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    45361 tgatctcgac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgccatac
    45421 agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgaa
    45481 aggtaactct ataaattaag taaaggagtg aaacagtttc acaagtaaaa tatccagtgt
    45541 gctccatctc attcttaata gatttattaa gatcatcttt ttagatagca ctttcatcag
    45601 gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac ataagggaaa ccagtattca
    45661 cgctggatca gcgtcgtttt aggtgagttg ttaataaaga tttggaattg tgacacagtg
    45721 caaattcaga cacataaaaa aacgtcatcg cttgcattag aaaggtttct ggccgacctt
    45781 ataaccatta attacgaagc gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg
    45841 attaactatg ctgattccgt caaaattaag tcgtccggtt cgactcgacc ataccgtggt
    45901 tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac ttccggctgg cgctgatcac
    45961 aagtcctgcg ggctacggta agaccacgct catttcccag tgggcggcag gcaaaaacga
    46021 tatcggctgg tactcgctgg acgaaggtga taaccagcaa gagcgtttcg ccagctatct
    46081 cattgccgcc gtgcaacagg caaccaacgg tcactgtgcg atatgtgaga cgatggcgca
    46141 aaaacggcaa tatgccagcc tgacgtcact cttcgcccag cttttcattg agctggcgga
    46201 atggcatagc ccactttatc tggtcatcga tgactatcat ctgatcacta atccagtgat
    46261 ccacgagtca atgcgcttct ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt
    46321 gtcacgtaac cttccgcaac tgggcattgc caatctgcgt gttcgtgatc aactgctgga
    46381 aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg
    46441 tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt tgcgatgacg tttccggttg
    46501 ggcgacggca ctacagctaa tcgccctctc cgcccggcag aatacccact cagcccataa
    46561 gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga
    46621 ggttttggat aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg
    46681 ctcaatgaac gatgcactca tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgcct
    46741 cgaagagatt gagcgtcagg ggctgttttt acagcggatg gatgataccg gcgagtggtt
    46801 ctgctatcac ccgctgtttg gtaacttcct gcgccagcgc tgccagtggg aactggcggc
    46861 ggagctgccg gaaatccacc gtgccgccgc agaaagctgg atggcccagg gattccccag
    46921 cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg atattctgct
    46981 taatcacgcc tggagtttgt tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa
    47041 ggccctgccg tgggacagtt tgctggaaaa tccgcagttg gtgttattgc aggcgtggct
    47101 gatgcaaagc caacatcgct acggcgaagt taacaccctg ctggcccgtg ctgaacatga
    47161 aatcaaggac atcagagaag gcaccatgca cgcagaattt aacgctctgc gcgcccaggt
    47221 ggcgattaac gatggtaatc cggatgaagc ggaacggctg gcaaaactgg cactggaaga
    47281 gctaccgccg ggctggttct atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct
    47341 gcactgcaaa ggcgaattga cccgctcact ggcgctaatg cagcaaaccg aacagatggc
    47401 acgccagcac gacgtctggc actacgcctt atggagttta attcagcaaa gtgaaattct
    47461 gtttgcccaa gggttcctgc aaaccgcgtg ggaaacgcag gaaaaagcgt tccagctgat
    47521 caacgagcag catctggaac agttgccgat gcatgagttt ctggtgcgca tccgagcaca
    47581 actgttatgg gcctgggcac ggctggatga agccgaagcg tcggcgcgta gcgggattga
    47641 agtcttatcg tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg
    47701 ctcgctggcc cgtggtgatt tagataacgc ccgtagccag ctgaaccgtc tggaaaacct
    47761 gctggggaat ggcaaatatc acagcgactg gatctctaac gccaacaaag tccgggtgat
    47821 ttactggcaa atgaccggcg ataaagccgc cgctgccaac tggttgcgtc atacggctaa
    47881 accggagttt gcgaacaacc acttcctgca aggtcaatgg cgcaacattg cccgcgcaca
    47941 aatcttgctg ggcgagtttg aacctgcgga aattgtcctc gaagaactca atgaaaatgc
    48001 ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg ttgctgctaa atcaactgta
    48061 ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat taaaactggc
    48121 gaatcgcacc ggatttatca gtcattttgt catcgaaggc gaagcgatgg cgcaacaact
    48181 gcgccagctg attcaactca atacgctgcc ggaactggaa cagcatcgcg cacagcgtat
    48241 tctacgagaa atcaatcaac atcatcggca taaattcgcc catttcgatg agaatttcgt
    48301 tgaacgtctg ctaaatcatc cggaagtgcc agaactgatc cgcaccagcc cgttgaccca
    48361 acgtgaatgg caggtcctgg ggctgattta ctccggttac agcaacgaac aaatagcggg
    48421 tgaactggaa gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt accaaaaact
    48481 cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag aaactgctga agatgatggg
    48541 ttatggggtg tgagtttgta tagcgttttt atgtacactc tttatactgt atataaaatc
    48601 gcactacagg ctcatgaata tgaaaccgga aagcaaagaa gcgccaatca atatcagagc
    48661 gaaagcctca cagcgtgacc tgattgatat ggcagcgaat ctggtcgcaa aatcgcgcac
    48721 tgattttatg ctggatgccg catgccgtga agcgcaagac atcttattag atcaacgact
    48781 attcatcctt gatgacgagc aatacgatgc gtttctcgct gccctggacg cccccatcac
    48841 tgctgagcgt caggccaaaa ttaacgcgtt gatgaatcgt aaatcgccat gggaataacg
    48901 gctcctactc ccctaacgtc tgaacataat ctggctgact tttgctgttc cgatcacggg
    48961 atgaatgaat ggctaaaaaa gaaagcgtta aaaaatcaca gctccggtct gtctcgtgtt
    49021 tacgttatct gtattgccaa tacccgccag gtaatcggct attactgcct ttctacaggc
    49081 agtattcaac gtaatttggc ccctggagcc atgcgacgta atgctcccga atcgttgcct
    49141 gttgtcgtat taggcagact ggcaattgat caagcctggg caggtaaagg gttgggcgtc
    49201 gcattgttga aagatgcagt ctatcgcaca atgtccatag ctcaacaggt aggggttcgt
    49261 gcgctcattg ttcatgcctt agacgattca gtacgcaatt tctatctgaa gtatgccttt
    49321 gtaccttcac cattccagtc acttacctta ctgtatccca ttactctgga gtaatttacc
    49381 tctaccggat agtcacactg atgagtctga tccgggaggg atttcaatca gtgagtttgg
    49441 ttatcatcac ccgcgttacg ccatctgttt cagccagagt aaaccgcacc ggcaagaaac
    49501 gctccaccac ggcgatattg gtcagcagat ggcatgaggg atgggcgacc gtaaattgtc
    49561 ccgcgcccgc cagcgccatt ggcaacacta gctggtcagc aagatattcc cccactgccg
    49621 ccgggcttgc caggtagcgt ttcacctctt tcaccaactg tgccgcaacc acctcggcac
    49681 tgacgcgctt ttcaccgacg acaaaaaagc gttcggtgat attttcactt tcgacttcaa
    49741 gcgagacggt attacccggc ccctggtcgc gcggcaggtt atgaatattc tgctcatgca
    49801 gggaaaaact tgccgccagc gtagcgattt cacgctcagc gacatgccgc ggcacgccag
    49861 ctaataacac ctctccacgc atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt
    49921 taaacgatgc caccggcgag acttccgttg ccaccacgcc gcctccggca ggataaaaac
    49981 cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc ggctccaaca
    50041 cccggcggat aaaatccgca ggcggggccg acgggttatc ggtaccgccg ctcacttcaa
    50101 cacgcgaagg tccatcggca aaccacagcg cgggcagcac cgtttgcagc accagcgtac
    50161 aacttcctgc gctaccgata gcaaagcgat aatcgccgcc acgcacggtg ccgggccgga
    50221 agaccagacg ctgcgacccc agctccgcgc cttccaccgt cgccccgcaa atttccgtcg
    50281 ccgcctttac cgccgtcaga tgctggcgca acagtcccgg ttttgcccgc ccggcacgaa
    50341 tgccggtgat ggtaaatggc tggccggtta tcatcgacag actcagcgcc gagcgcatga
    50401 tctgcccgcc gccttcgccc tgtgcgccat ccagcgcaat catccttttc atcatttatc
    50461 cttttacgca caccacctga cgtagggtgt agataacttc caccagatcg ctttgtgccg
    50521 ccatcaccgc atcaatatct ttatacgcca tcgggatttc gtcgatcact tcggcatctt
    50581 tacggcattc cacatgcacg gtggcacgaa tttgatcttc cacgctgaac atttttttcg
    50641 ctttagtacg gctcataacc cgcccggcac cgtggctgca cgaacagaac gactcttcat
    50701 ttcccagccc acggacgata aagcttttcg ctcccatcga ccctggaata attccatatt
    50761 gaccagcacg cgcagacacc gcgcctttac gcgtcacgta gatctcttca ccaaagtgct
    50821 gttctttttg cacatagttg tggtgacagt tgatctcttc catcgccagc gtttgtggct
    50881 gtctgaccgt tttctgcgta atgctctgca acgccgttac tacgttttcc atcatcacat
    50941 cgcggttaag gctggcaaaa agctgcgccc aggccacggc tttcaggtaa tcatcaaagt
    51001 attccgtacc ttccataaag tacgccagat cacgcgacgg caatgtttca agcgtttcct
    51061 gcatctcttt ttgcgccaga tcgataaagt aagtcccgat ggcattacca attccgcgtg
    51121 aaccggagtg cagcataatc cacacctggt ccgactcatc aaggcagatt tcaataaagt
    51181 ggttacccgt tcccagcgtt cccaggtgtt tatagttgtt agtgttaagg aaacgcggat
    51241 atttttgcgt taaccactga taaccggcct ccagctcagc ccatttcgca tcgacgttaa
    51301 caggcgggtt ttcccatgcg cctttatcac gtttacaacg gccagtggta cgcccgtgcg
    51361 gcaccgcagt ttcaatcgcc tgacgcagct ccgccaggtt ttccggcagg tcagccgccg
    51421 ttaaagcggt acgtagtgcg ttcattccac agccaatatc cacgcccacc gccgccggaa
    51481 taatcgcccc tttggtcggg atcacgctac caatggtgga acctttaccc aggtgtacat
    51541 caggcattac cgcaatatgt ttgaaaataa acggcatctt cgccgtatta ataagttgct
    51601 gacgcgcatc ggcttctacc ggcacgcctt tggtccacat ttttaccggg gcattttcag
    51661 tggtcagtaa ttcgtaattc attttgtttt tctcttttcg ttgtttgcgg tcctgataat
    51721 tgcaaccgtc gtgccagaaa attaaataag cagattaatt ttttaattca ttgttttaaa
    51781 aagattatat ctttacgtcc gtaaacggag atttcccgca aagccaattt accgataatg
    51841 aaatatcgtc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    51901 gaacagtgct ggattatgcc gggcgcggca gtcagcgctg gtcgaaatgg cgtccgtcac
    51961 tctgtatatg ccagcaagaa tcgttagtca tcgatcgact ggaattattg catgacgccc
    52021 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaaccg
    52081 aagtggtgag cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    52141 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    52201 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggct gaagcgcgtt
    52261 acctgcccgc ccggttgata caatcttcac ctccgcgcaa aaaagaacag ccgcgcggcg
    52321 caggtgaagt gacgattatc gatctcgatt taagccttta taacgccatc gccagccgct
    52381 ttgccgagga acgccagcaa acgcttgatt ttcttaagtc cggtattgcc acgcgtaacc
    52441 cccacttcaa ccgcatgatt gagcagattg aaaaagtagc gattaaatcc cgcgcaccga
    52501 tcctgctaaa cggcccaacc ggtgcgggca agtcatttct ggcgcgacgt atatttgaat
    52561 taaaacaggc gcggcatcag tttagcggcg cgtttgtgga agtgaactgc gcaaccctgc
    52621 gcggcgatac cgccatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcac
    52681 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    52741 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agccattgaa gaaaaaacct
    52801 tttatccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    52861 tgcgtgattt gcgccagctg gttgccgatg gcaaattccg cgaagatcta tacgcgcgga
    52921 tcaatctctg gaccttcacc ctgccggggc tacgccagcg tcaggaagat attgaaccta
    52981 acctggatta tgaagtggag cgccacgctt cactcaccgg cgacagcgtg cgttttaaca
    53041 ccgaagcgcg gcgcgcctgg ctggcctttg cgacatctcc ccaggcaaca tggcgcggta
    53101 actttcgcga gctttctgcc agcgtcacgc gaatggcaac ctttgcctct agcggacgca
    53161 ttactctgga cgtggttgaa gatgagataa accgtctgcg ctataactgg caggagagcc
    53221 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    53281 gtatgcaact ggaacacgtt atcgcaatct gccgccaggc aaagtcgctt tccgcagccg
    53341 gacgccagct ttttgacgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    53401 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    53461 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggtgtcgg
    53521 tgtagacggc atctaccatg ctgatgctac ccatattgac catcgcgtta cggccaaatt
    53581 tcgagtgatc gacaaccagc ataacgtggc gcgagttctc aataatggcg cgtttggtgc
    53641 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct atcgatgccg cttatcccca
    53701 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    53761 cgccatcgcg gctgcgtaat tcgccaccgg cgagaataat acggaaatcc tcttttacca
    53821 tcagcgtgtt agcaacgtta agattgttgg tgacaatgcg caaattgctg tgattgagca
    53881 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    53941 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    54001 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcaccgcca tgatggcgca
    54061 ggatcaggtt ttgctccgcc agctcattga ggtcgcggcg aatagtctgc gggctgacgg
    54121 agaagtgctc caccagctcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    54181 taccgttgtg acgttgtgtt tgtttcattt ctaaatccct ggaattattt tcgttttcgc
    54241 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaacccggc gatgtgtgct
    54301 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    54361 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    54421 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    54481 aatttttgct gcacatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    54541 ccgctaccga ggcgtttttc caccgcaccg ccgagatacc accaccagag caggttaaag
    54601 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    54661 ttcagtgctg gatcgaatgg ccaagccagc cataacatca cttcctgatc gccgagaatt
    54721 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    54781 cgttcacgca aggcggcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    54841 cctgcctgcc agctcgccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    54901 agctccgccc gtacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    54961 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    55021 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    55081 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgacgttgcc
    55141 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    55201 gcgccgcgcc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    55261 cgttattacg cataaaagcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    55321 gtcccattgc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    55381 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    55441 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    55501 aatgttatac atatcactct aaaatgtttt ttcaatgtta cctaaagcgc gattctttgc
    55561 taatatgttc gataacgaac atttatgaac tttaacgaaa gtgaatgagg gcagcatgga
    55621 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    55681 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    55741 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    55801 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggccc cgcatatcgc
    55861 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    55921 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    55981 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    56041 cgactgttgg gtagacgatg cccgtctggt gctcgccaac gcccagatgg tggtgcgtaa
    56101 aggcggcgaa gtgcttactc gtactcgtgc tacttctgct cgtcgcgaaa acggcctgtg
    56161 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    56221 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gacgggatgc atctgccttc
    56281 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    56341 aaagcaggcc tacattctgc aaaacgaaga taaacgtatt gtgttcgtga tcccgtggat
    56401 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggcgatc cgaaagcagt
    56461 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    56521 acagcttagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    56581 tgagtccgac tcgccgcagg ctattacccg tgattacacc cttgatattc atgatgaaaa
    56641 tggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    56701 ggaacatgcg ctggaaaaac taacgccgta ttatcagggt attggcccgg catggacgaa
    56761 agagagcgtg ctaccgggtg gcgccatcga gggcgaccgc gacgattatg ccgctcgctt
    56821 acgccgccgt tatccgttcc tgaccgaatc gctggcacgt cattacgcgc gtacttatgg
    56881 cagcaacagc gagctgctgc tcggcaatgc gggagcggta agcgatcttg gggaagattt
    56941 cggtcatgag ttctacgaaa cggagctgaa atatctggtc gaccacgagt gggtccgccg
    57001 cgccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    57061 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    57121 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    57181 aattgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatatg ttgaatttgc
    57241 atgattttgt aggcctgata aggcgttcac gccgcatcag gcatgaacaa agcgccacgt
    57301 tgtcaacaat ctgacctact ttttgattca gggaattacc gcaaagccca cgccatcata
    57361 cccaacgtaa caaaacttaa gcacgttccc cactcactcc acttccgtat ctggcttcta
    57421 tttttgctac gccactggca ataatatccc aacgcaaaac caagctgtgc acttatcgga
    57481 ccgacaaagt cgatccactg ttgagaccaa aatggcgaaa aattcagtgg gagccagaca
    57541 actgtcagaa agaaactgcg catattttcc agcaaagcaa tttgcgctgg cgcgacataa
    57601 aaaatgatat tccacaggag caaccagaaa taaactggtg caaataccag ccaacaatgc
    57661 agtctgcttt tcaattcagg tggcgttctg cgcgcaaatt gccaaccaac gcccaccatt
    57721 gcacagaaga aaagcgcata ttcagccgat aaccatagtt gtaattgcag aagggaaata
    57781 cgcctgtgca tgagcgggat tgagaacagt atttctccca gaaaactcag cgtgattaca
    57841 caggcagcca gcgcaaccac cgtcggccac accaacaacg gtacaataac aaagccgaga
    57901 aataacggat aaatataaag tctgattata ttcatcatta ttgtcctgat gattcggggg
    57961 atgcttcata cagcggcaca taccccacat cctctaccaa ctgttgcccc tgctgactta
    58021 taaaccagtc aacaaatttc tgcgtttccg gcgtgggatt ttcacgcgtc accatatagg
    58081 catccacaat gtatggataa gtgccgttgc ggatattttc ctcggtaggc gcaacgccat
    58141 ttatagccaa aagtttaatc tgcttgcgca ttttgagcat attgtcattc atacgagtga
    58201 cgtaatagtg gaaggtgtac ccaatggaag atggcgtgtt ttgatagtcg gcaacttcag
    58261 taattaaacc gcccataaga tcaatcacag tggatttttt agctggcagc attggggtgt
    58321 cttgcataac caaccccttc ataatcgttt ggcttccaga atcttcaggg cgctgccaga
    58381 cttttatatg ttcgtcacta ccacctactt tattccagcg gctagttttg ccgctaaaaa
    58441 tatctttaac ctggtgctca gagagagaat ttaccgggtt attaatatca acgataaaga
    58501 caaaggcttc gcgggcgaaa gcggtatatt tcagtttaac acccgcttct tctgcacgtt
    58561 ttttctgtcc ttccgacggt tgtgcaacaa aaataaccgt cgcggaacct ttaatcagac
    58621 tatcgtaggc ttcagctgtt cgggtttgct gcaaaccata cgcctgccag gccagcgagg
    58681 ttcgatcctt atcttttggc tcgggtacta atttataata cgcggaggca tacagcgggt
    58741 ataaggctgt agcgccgtac actctcggcc atcttgacca atcatcgctg taacgtaatg
    58801 caggttgccc ccgcagagac gtcagtccat ggctctcttc ttccctgacg ctgttatagt
    58861 acccttcccg gtcgacacat cccgccagta acgccgtgcc taatagtaaa acaccggtga
    58921 atttattcat ctcgctgatt cctttctctc gagcgttatt ccattgttag atacagcggc
    58981 acatatccca catcttctac cagactctgc ccctgcggcg ttaaaaacca ttcgaccagt
    59041 ttttgtgttt ctgacgttgt attttctctc gtcaccataa atgcatcgac gatgtacgca
    59101 tatttgccgt tgcgaatgtt ttccgccgtc ggtgtaatac cgttaatcgc tagcaatctt
    59161 atatttttat cagcattcat ttgcgtcgcg taatagcgga aggtatagcc tattgcgttg
    59221 tttgtattac ggtattcggc aacgacttta atcattccct ccatcacgct tgccacttcc
    59281 gtttcctgcg gcgagatcat gcggactttt ttcatgacct gtgattgcat cactgtctgg
    59341 ctgccagagt cttccgggcg ctgccaggtc tggatctcct gatcgttacc gccaacagta
    59401 cgccaattgg taattgcacc actgaaaatg tcacgcacct gttgttcagt cagggaatta
    59461 accggattat ccgcattgac gatgaaaaca aatgcttcac gggcaaatgg cgtgtatagc
    59521 aaagtgacgc ccgattcctc agcgcgtttt ttctgcccgc cggagggttg cgccacaaaa
    59581 ataatatcgg catcaccttt aacaatccgg ttatatgcat cgggggtacg agagttctcc
    59641 agatattccc aaacgtgaaa atcctctggt attacactta atgcataaaa tgcagaagca
    59701 taaattgggt acgcagccgt ggcgccatcg attcgcggcc agttttgcct aaactgaatt
    59761 tgcggtttgc cacgcagcgg cgtcagtcga ttattgagtt tatcgggtcg ccaggccctg
    59821 atatcaatat tttcgcgtac accgacacca ggattttgct gcttatataa atgggcctga
    59881 tacccggcga caataccaag taagagaagt agaaacagga gtaagttacg gcaacgtagt
    59941 ccggacgtgc cggataaagg atatctggtc ggccaggcgt atcccagcga aaaactgata
    60001 tgcgccccaa cgggaataat gaccatccac cagtactcac cacccactaa tgagactaaa
    60061 aaaacgccca accagggaag cataaggttt gccaaccaac cagctcccat ctggcgaaat
    60121 tgataatctg ccagatgtag cgtgattatc cagccaacca gggttaatag cgcgggtaag
    60181 agaaaaaagc attgccgcca gacggctatt tctctgtttt ctggcgcgct atcaccggcc
    60241 cacacgcgac cagagaaaaa tattattatc gcccccagcg tcagcataaa gacgttgagt
    60301 agaagataaa ttgttggcgt gataaggata ttacaaactt caataatacc ggtaagcccg
    60361 acacccagca tggcaacaac cgctgccaaa aattgcgcca gtatggggat gccgaaaaaa
    60421 gtcattatca gcgaggtcaa aatccatttt ctatttggca tcattctgtc cattcttcct
    60481 gaatgacgga attatactcc ccgagaaccc tcgccccctc tggacacttt tccgaaataa
    60541 aggcgaaaaa aacgggacct tgtggccccg ttctgttttt tagcgaactt acaatctcac
    60601 cggatcgata tgccagatat gatcagcgta ctctttgata gtacggtcag aagagaagta
    60661 gcccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag
    60721 ttcatcgact ttatcctgac aatcgacata gctgcgataa tccgccagta cctggtagtg
    60781 atcgccgaag ttgatcagcg aatcaaccag atcgcgatag cgacccggat cttccggact
    60841 gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta
    60901 ttcacgcggt ttgtagccct gacgacgcag ttcttccact tcttccgctg tgttaccaaa
    60961 aataaagata ttgtcagcac cgacatgatc cagcatctcg acattcgcac cgtccaacgt
    61021 accgatagtc agcgcaccgt taagcgcaaa cttcatgtta ctagtgccgg aagcttccgt
    61081 ccctgccagc gaaatctgtt cagacagatc tgccgccgga atgatcaact gcgccaggct
    61141 aacgctgtag ttcgggatga acacgacttt cagcttatcg ccaatctgcg gatcgttgtt
    61201 gatcactttc gctacgtcat tgatcaaatg gataatatgc ttcgccatgt aataggccga
    61261 agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatcca ggtcggcctt
    61321 gatgcggtta tagcgggtaa tcacatgcaa cacattcatc aattgacgtt tgtattcgtg
    61381 aatacgtttg atttgcacat cgaacaacgc ctttggattc accaccacat tcagctgctg
    61441 ggcgatatac tccgccagac gctttttatt ctccagcttc gcctgatgca cagcgtgatt
    61501 aaccattggg aaatcacagt gttgttgcag ctcattaagc aggctaaggt cggtgcgcca
    61561 gttacggccc aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta ccgccagcca
    61621 gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa
    61681 gtcggcaaac aacgattgca ccatcagatt agagtgcagt tccgatacac cgttaacttt
    61741 gtggctcaca acaaccgcca gccaggccat acgcacacga cgaccgttgg attcatcaat
    61801 gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag
    61861 gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat
    61921 atcaaccggc caggtttcca gcgcctcgct catcagcgtg tggttagtgt aggagaagac
    61981 ctgacaacac acctcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg
    62041 catcatctca ggaatcgaca gtaccggatg ggtatcattg agatgaatcg cgattttatc
    62101 cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt
    62161 cgaggaaacc aggaagtatt cctgacgcag gcgcagctca cgcccggagt aggtggagtc
    62221 atccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa
    62281 gtagtcaccc tggttgaatt taccgaggtt aatttcgcta ctggcttgcg cactccacaa
    62341 acgcagcgtg ttggtcgcgt cggtgtcgta accagggatt atctgatcgt aagcgactcc
    62401 cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc
    62461 aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac catattccag
    62521 ccagtagtca ggcgactctt tctggctacc gttaacgatg ttctgcttga acataccgta
    62581 gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcgaggaa
    62641 gcaagccgcc agacgtccca ggccaccgtt accgaggccc gggtcatttt cttcatcaat
    62701 cagctcttcg agatttaacc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc
    62761 tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta
    62821 aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag
    62881 acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg
    62941 gtcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct
    63001 aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt
    63061 caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc
    63121 catagcctga cgttgcacaa accgccacag agaaggacgg gaccacagta caaaagcacg
    63181 tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac
    63241 gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg
    63301 ccgcactaac ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc
    63361 aaaacggctg ggcaccagaa tgacgtccgc accgcccata atgcgatgcg aaaatgcttc
    63421 gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg caaggaaacc
    63481 ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag
    63541 acccggtaag gcctccagca ccaggtcgag gcctttctgg ctggtcagac ggctcaccac
    63601 tgcaaaaagc ggcactttat cgtcaacctt aagccccatt gcgatttgta actggcgctt
    63661 attttccgct ttatcttcca acgtatcgcg ggtgtaacgc gaggccaata gtaagtccgt
    63721 ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg
    63781 atgacgctgt tgcaacaaac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg
    63841 agcgtaggtt ggactgaccg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa
    63901 agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat
    63961 gtcattcatg tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca cagtaaacac
    64021 cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc
    64081 gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc
    64141 aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc
    64201 gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac
    64261 cccgttgtaa tgaccgaaca acagcgtgat atgcccggcg aaggtatcac gacgggatac
    64321 tacctgcgca tcggtcacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc
    64381 gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc
    64441 ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt
    64501 ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga
    64561 tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cggaataaca
    64621 caagcacgat cgatgacgca gcggcgcaga cggcacgagc gacctaccca tacttccggt
    64681 aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg
    64741 gactgcacca ccaccgaacc ggagatcaca caaccgccgg aaaccagtga gttaagggtc
    64801 atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag
    64861 gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg
    64921 aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc
    64981 ggatcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg
    65041 atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcgcg atcgtcttct
    65101 tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat
    65161 ttgctcggat cgttcggcat tgacggcggg ttagcaggtt tttcaacgaa ttcgataatt
    65221 ttatcgttct catcaaccgc cataacgcca aatgcggagg cttcttcaat cggtactggc
    65281 atacaagcaa cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat acgcgagtag
    65341 tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcga
    65401 ataatatcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc
    65461 attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt gaagaatgac
    65521 cagccgcgct gaatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata
    65581 cgacggatcc cggagttgat gcagttagac agcgcaaagt cgataatgcg gaacttaccg
    65641 ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt
    65701 cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgcgccaa cattaagtga
    65761 tcgttcttct ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca
    65821 atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg
    65881 ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt
    65941 taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc
    66001 actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt
    66061 cttcccacca gcgattctcc accaaagcgg gaatacgctt gcgcagatgg attaacgcgg
    66121 cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat
    66181 cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta
    66241 acatcggcgt accctgggag agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc
    66301 gccgttcaac caggtcaagg gtaccgccta ccccttcttt accatgattg ttactgtaat
    66361 tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaaac
    66421 aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg
    66481 gcagacgacc attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc
    66541 ccagaggcaa atcataatgt agccagaaac gacgggcagc atcgcggaaa tgatcgttcc
    66601 actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg
    66661 gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg
    66721 gcgcatcctg acggaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac
    66781 catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa tccaccaccg
    66841 ccgggtgact caaattgagc gtgttgccac aaccggtcca gttgtgataa tcgccgtctt
    66901 ctcttatcca ataatagcta cggttgtcga tcccacgcag cgagaataac gggccgtcga
    66961 ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat
    67021 gcagtgcttt gattgcatcg cgaaactcat gcagcgccgt ttctggcgag caggcatacg
    67081 ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttactt agccccatgc
    67141 gttgcagacg tggttcactg gcaaactgcg ccactggcag cagttccagc gcggtaatgc
    67201 ccaattgttt caaatagttg atcatcaccg gatgcccgag ggctttataa gtgccacgga
    67261 tctcgaccgg gatctccggg tgcaggtacg ttaatccttt aacatgggct tcataaatga
    67321 tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaacca
    67381 ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg
    67441 cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca
    67501 acaacttcgc cgggttaaag cgatgcccct cggcgggttg ccaggggcca tgaacgcgat
    67561 aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt
    67621 gccctggcaa gtcatagcga tgttcctggc cattggcgtc aaagacacac agttctaccc
    67681 gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga
    67741 ggggagcggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt
    67801 ggccagcggt ggtagcgtca ggcttagtga atgctgacga ccgtggctgg caatctcatc
    67861 gctgtgtacc gtgccgccat tacctgcatt actgccgtga tagtgcatgg aatcggtatt
    67921 gaggatctca cgccatttgc ctggctgatt aatgccgaag cgataatcat gacgcggcac
    67981 cggcgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat
    68041 cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc
    68101 cagttcatgc atcgctttat ggtggcggta ggtgaggttc agatcgcgca ccagacgctg
    68161 gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg
    68221 gttccactcg cggccctggg caaattcgtt ccccatgaac agcagtttct tgcccggaaa
    68281 tgcccacatc cagccatagt aagcgcgcag gttagcgaat ttctgccatg cgtcgcccgg
    68341 catgcggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac
    68401 gaagttttca gtgtagttgt agagcatccc gaaggtcagt ttatcgtgat gatactgacg
    68461 ataaatcggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt
    68521 gtaccagaag cccagaccgc ccatatcctg cggacgagaa acgccaggga aatcggtaga
    68581 ctcctcagcc attgtcaccg caccggaaac ctgctcacca agaatacggt tggtattacg
    68641 caagaattca atcgcttcaa gattctcgcg cccgccaaat tcgttcggga tccactcccc
    68701 ctctttacgg ctgtagtcac gataaatcat cgacgccacc gcatcgacgc gcagcgcatc
    68761 aataccaaaa cgttcaatcc agtaaagcgc gttaccgacg aggaagttac tgacttcacg
    68821 gcgaccatag ttgtaaatca gcgtgttcca gtcctgatga tagccttcgc gcggatcgct
    68881 gtgttcatac aagttcgtac catcaaattc ggcaagcgca aagtcatcag tcgggaagtg
    68941 gcctggcacc cagtcgagaa tcacgttcag accagctgcg tgtgcagcat caatgaaata
    69001 acggaagtcg tcgcgagtac caaaacggcg ggttggtgca tacaggccgg ttggctgata
    69061 accccaactg ccatcgaagg gatgctcgtt aaggggcagt agttcgaggt gggtaaagcc
    69121 catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt
    69181 gttgtcggtg tggcgacgcc aggagcccag gtgaacttca taaatagaga ttggcgcatc
    69241 aaactgattc gcttttttgc gctcttcagt ctgtacaacc ttttccggca gcccgcaaat
    69301 aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg
    69361 caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg
    69421 gataaacagt tcccagatgc cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc
    69481 ccagtagttg aattgcccaa ccaccgagac ccgacgggcg tttggagccc agacagagaa
    69541 acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaagttt catacgggcg
    69601 caggtgagta ccttcagata atagccaggc atccatttcc tggattagcg gaccaaaacg
    69661 gtaaggatca tcaatcaggt tttgctgccc atgccagaca acagccaact gatagcggaa
    69721 aaaattctta cgtcgcggaa tgacgccact aaagaatccc cgtgagtcga gacactccag
    69781 ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa
    69841 aagggcacgg acttccagtc ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc
    69901 cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct
    69961 tgtcttcctg ttttattgtg tcaccccatc caattggatt tttgacttct ggttgtgaca
    70021 tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag
    70081 gtaaggctgt gaatactcat gtattcagcc acccttaaag aatagccaat gctctattta
    70141 actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta acaggggcgg
    70201 catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc
    70261 ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag gaactctggt
    70321 cccatattca gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt aacggcagct
    70381 ggggttagct cacgcatagt gatttcccga tcgttcggaa cgactttcgc ccacggattg
    70441 tgcgcagcca gcagttcttc cacggtcgga atcgacacat cttttttcaa tttaatagtg
    70501 aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc taccggaatt
    70561 acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc
    70621 tgaccgttat cgagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca
    70681 aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg
    70741 agaatagcag aggacggggt cgcgagttca tctgctacat ggccatacag atggcccatc
    70801 tgggttaata actcacgcat atgccgcgca ccaccgccgg aagcggcctg gtaggttgca
    70861 acggacaccc aatcaacaag atcattggcg aataaaccac ccaacgacat caacatcagg
    70921 cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg
    70981 acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagacgat
    71041 gctgcgtcaa tccagtaacc ctgccatccg ctttcacgaa gctttggata gatttcgttg
    71101 gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca
    71161 aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg accaagctga
    71221 gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc
    71281 atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt
    71341 ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca catctttcac
    71401 catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta
    71461 atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg
    71521 agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg atccgctcgg
    71581 aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat
    71641 catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga
    71701 gaaaattctg gcatttctca gcctgcgagc agaaaccgtc tccatttctg gcggcatcat
    71761 tctgtttcta attgccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct
    71821 tccggcaggt gaagagccat ttatcgtgcc gctggcaatt ccgctggtcg ccgggccaac
    71881 tattctcgcc acgctgatgt tgttgtctca tcagtacccg aatcagatgg gacatctggt
    71941 gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt
    72001 tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct
    72061 ggtgatgatg gcaacccaga tgttcctcga cggcattcga atgtggatga aggggtaagg
    72121 ttcaaacagg tgctgcacac aaccagacgc ggcacctgtt ttattacctg aactgataat
    72181 gaccttcgcc caatcggata acccttccct cttcttctgc gcggttcagc cacacactaa
    72241 tttgttcgat ggttaaacca gtacaagacg ccagcctttc aatacttatc gattcctttg
    72301 caaggatcgc cagttccgcc acaaagagtt gataaaaatc gacaggcgta tacgcgggca
    72361 ttactgcgta atttgcgtca tcttcatgca gcgaaaacag ttctggttga gcgttacgga
    72421 gtgctctgtc atacatcagc ccaggaacgt ctggcagtct gatcgtcaca ttctcagcct
    72481 gttcagcact ccatgacgcc ccattttcta ctaatcgtga attggctgat tgcatatcct
    72541 gatttggttt aacctgcaca ggcagccact gatgttttaa tgcctccatc gccccggtta
    72601 tcgttccacc ggtcattccc gcacgtacaa ccattgcgct ttcagcaagg caataaatat
    72661 atttatttcg cgccatcgca ttgccgacgg tgaaacgtac ctctgggtaa aacggcgaaa
    72721 tcaacaccag gttgcctgct ataagccctt cacgccattt cactaacgta ctcgttttta
    72781 acaagctatc agctaatacg ccaacggcag ttcccccggc ctccagtgcc gacgccattg
    72841 cacattcatc aatacctcgc gcaccaccag agataacgca aatcccctgt tgggccagtt
    72901 tcgcggccag ttgttgggta tagcgcaaat cgtcagtcgg agcatctcgc gagccaacaa
    72961 tcgccatacc ttccgcttgc agtaatgctt tattcccgca gccaaataaa acgggaggtg
    73021 catccgttcg caatcggttt ttcaagcgaa cgggataatc agcatctccg cgggttaaga
    73081 tccataaacc ggcgcgttgc cacttatcta ccgccaacgc cagactatga ccacgcgcca
    73141 gcaagccaag aatacgctcc tgagaaatac gcggatcgct ccagcctgta agtttctctt
    73201 gcggttgcgg caccagaagc tcggcgggac tgatacgttg gtgcttcagc cataatgcaa
    73261 acttccccca ttcactatta ctaagaggtt tatacttact cgccgccgcg cgagaaaaat
    73321 cgctggttag caggagagtt gcttgtgcat tggctgaaag attcatcaat tttttaccga
    73381 ggtagaagca agggcaagag gataaaccgt cgggcaacct gcctggcgta acagtgctgt
    73441 tagaactgtc agcgtccatg cagaatccac gatatcgtca accagcaacg ccggaccagg
    73501 catcaaagga ggggtaatca caaacgcccc gtcgagattt tgacactggt ggaaacggtt
    73561 ttgctgcatt ttctgcggtg gattgtccac gactttttca atggcatcaa taaaaggtaa
    73621 gccaagtttc gccgccagtc gccgggcaaa atcaggaacc aggtcgaggt gctttcaaaa
    73681 tcaggaacca ggtcgaggtg ctttaatgaa ggaacgcagc atacccaggt tggttcagga
    73741 tgcggattcc agcgttggcg aaccatttcc gcacatgcct ccaccagttc atcactgaat
    73801 cgccccgttt ttttctcctg tgctacctgc tttccccacc cggaatatcc ccaacgagaa
    73861 aggattcgcc ccgtagatcc ttgtaaactc gcaggcaagt tccctttaaa tccgtattgc
    73921 gtaaaagccc cagctgccac ctgtttattg agatttaatg acaggtcagc gtgtttaata
    73981 aataagtttg ccgcatggag taagccgcta tcaatgtcgg gcgacaacaa cggatgctga
    74041 agacaactgc tacatttacc acaatgttcg gcacctggct catcgagtgc gcggcgcaga
    74101 aattgcattt tgcactcctt ggtcgtgata tagctttcaa cgtctgccag ctcacttttt
    74161 ctctgattca tcaaatggtt aattcgttca tggtcaggag aaaaactgac aattgttctg
    74221 cgccataatt tctcggtata caccacaggc gatggatttt cagctaccaa taatttcagt
    74281 gctttttcta tttgcccata acggagattc gtccgttgtt caatgcctcg taaggtaaga
    74341 ccgtcattca cgctaagtac gttgagtatt tcatgaattt gcgcctccgc aggaaaggcg
    74401 ctttcacgga agaatttatg aatagcacgg tcttcactgc cgcaaagcag tatgccaaca
    74461 gcagaatcga taccgcgacc ggcacgcccc acctgttagt agtaaccaac tatcgaacct
    74521 ggcatctggt aatgaataac aaaccccaaa tccggtttgt caaagcccat tcccagcgcc
    74581 gttgtcgcaa ccagcacttt aattttattt gccagcaacg cctgctccag atattccctg
    74641 gcagtattag aatcttccgc gccttcacaa gtcacgccgc tgtagtaagc aaatgcactg
    74701 ataccatttt tccttaacca ctcggcgaca agttcggcat cgcgagtagt cagggtataa
    74761 acaattcccg atttggaaaa ctgagggata accgttgcta accatgccag acgggatgag
    74821 tgttcaccta agactaaggc atcaagcgcc aaactttcgc gagccagcgt tccacgctga
    74881 atcacaatgt cacccaattg ctgacggata tcctcaacga cacggttatt cgctgtcgcg
    74941 gttgtaccca gaacaggggt attcgcaggt agttgcgcaa aatatctaat atacgtcggt
    75001 aatccgggcg aaaatcgtgg ccccaatcag agatgcagtg cgcctcgtcg accacaagca
    75061 agccaatatg atcggctata ggatataaaa ccgtttcgat aaagtcctgg tttgccaaac
    75121 gttccgggga aaccagcaag caatcgacct caccttgcag caacttatcg ctaatgcgct
    75181 gccattcttc tctgtttgta gaattcaacg tttcagccgt aatacctaac cgttcagcgg
    75241 cggcaacctg gttacgcatc agggcaagca aaggagagat gataattgtc gggccagcgc
    75301 cgcgatcgcg gaagatttta ctggcgatga agtacacagc acttttcccc cagccagtgc
    75361 gttggacaac cagcagtttt cgccgttggt tcaccaccgc gtctatggct tcccattgcc
    75421 cttccctgaa agtagcggtg gtatttccta acatccgctg taaaagcaat tcagctgcgt
    75481 gcttttccat ctttatctcc tgcgatcttt aggcagtgat tagaacggat tatttgcagg
    75541 taaatttgcg agagagatca aaaagatgaa gagaggagaa aagagaaatg aattactgcg
    75601 agcaagctcg cagtaatttg tcgcagtaat taactcaaca gctggaacgc aatcatccca
    75661 acgatggcac cgacagtgcc gaggatggtt tccatcatgg tccaggtttt cagcgtttcg
    75721 gcttcggtcg cgccggtaaa tttaccgaac aaccagaaac cagcgtcgtt aacgtggctg
    75781 acaacaatcg aaccaccggc gatacaaatc gacagcgccg ccatttgcgc accggagtag
    75841 ttcagttgtt caataaccgg catcaccagt cccaccgccg ttaaacaggc aacggttgca
    75901 gaaccctgaa tgatgcgcac tgcagcggcc agcacgaagc aggtgatggc aatcggcagg
    75961 cccatgccgg ttaacgcttc gcccagtgcc ggacctacgc cagagtcaac cagcacctgt
    76021 ttgaacacgc cgcccgcacc aatcaccagc agaatgatcc ccgccggttg cagcgcgtga
    76081 ccgcaaatct ccatcacttt gtcttttggc atgccctgac gcattgccag gccataaatc
    76141 gctaccagac aagcaaccag aatcgcggta aacggatgac caataaactc gaaccattcg
    76201 taagcggtag agccttccgg cacaaaacgc gcggcaatgg ttttcagccc taccagcacc
    76261 agcggcagca ggatcagcga caggctgaat ccgaaggatg gcattttgcc ttcgccgaga
    76321 tgcggttcgc tgatgtcgtc aggaatatgc agctcaacgt aacggctgat gaagttaccc
    76381 cacagcggcc cggcaataat cattcccgga attgccgcac acaggccaat caggatcatc
    76441 cagccaaaat cggcgttcat ctgcgatgcc agcagcattg gcgctggccc tggcaccagg
    76501 aacgccgccg cggccgccac gcctgcgaat aatgggatta ccagcttcac caggttcgta
    76561 ccggtgtggc gcgccattga gaaagcaacg ctaatcagca gaacaatcgc cacttcaaag
    76621 aacagcggta gcgcacagac cagccccgca aggccgatgg cataatgcgc gcggctgtga
    76681 ccgaaggatt taagcatttt gacggcaatc tgatcgactg cgccggtttc atgtaagatc
    76741 ttgccaaaca tagctcccag ggcgacaacc accgccagga agccgagggt gcctcccatc
    76801 cctttttcca tcgtcgctgc gattttatcg agcggcatac cggaaaaaag gccagccccc
    76861 atggacacca ccattaaagc caggaaagcg tgcatacgcg ccttcatgac taaaaacagc
    76921 agcagtaaaa cagaccctac tgctgttaaa acaagcgtta atgtagtcac tacttatttg
    76981 ccttttttaa taacctcaat ggtgcttgcc acaacacctt ccagcggttg atcgatatcc
    77041 accaccagta catcggtttc gtccgcaccc ggctcctgca gcgtttcaaa ctgcgtcacc
    77101 aacatttggg ttttaaagaa atggcctttg cgcgctttca ggcggctttc aatcacatca
    77161 aaatcgcctt tcaggtagat gaaagagaga ttcggattac cttcacgcag caagtcacga
    77221 tagtgttttt tcaatgcaga acagacgatc agcgacactt tattagtgcg ctgcatagca
    77281 aacgcggcgt cgttcagcgc ctgcaaccac ggtttgcgat cgtcgtcatt cagcggttcg
    77341 ccagacgcca ttttttcgat attgcgccgt ggatggagga aatcgccatc aagaaacgcg
    77401 gcatgaagtt gatgcgccac ttcacttgcg accgcagatt tgccgctgcc cgatacgccc
    77461 atcaagacgt aaatgtggtg atcatggtta gtcgtgctca aagtggtgcc cccacaatac
    77521 aagaattaaa aatgttacgg gtaactgtta tcggtaacat tgtccagccg gacaatatca
    77581 gaagcaatat ccatccgtgc cttaagtgta taagtgtgag ctacttcaaa tttgtgggct
    77641 taaatagatc cgcccggtga caaggtgaaa cctaaatcta acattttcgg tgtcacagat
    77701 tcgccacgaa tacgcgccag caggcgttca gcgccaatac tgcccatccg ctcacgcggc
    77761 gtcagcacgc tcgcaagtcg tggctccatc acctgaccaa tgtcatgacc gtggaaaccg
    77821 gcaatcgcca tatcgtcagg aacttttaac cccagacgct gacattcaaa cgccgcgccg
    77881 accgccagat catcattagt acagaacacg ccatccagct gcggatattc ccgccgtgcc
    77941 tggcgaatca gttcaatacc ggaagagtaa gaagaagatt gctcaaccat cacgctatat
    78001 ggcaccaggc ctgcatccag catcgcctgt tcgtatccct tctgtttgat gatagtacgt
    78061 tcgtcgagac gtgcgccgag ataggcaatg tggcgatgcc cgcgagcaat aatggctgtg
    78121 gtcatctggc gtgccgcttc aaagttatca aaaccgacgg cgatatcgag acacggcgac
    78181 tggctgtcca tcaactccac caccgggatc cccgccactt caatcatctt taaggtgcgc
    78241 ggcgtgtggg tacgttcggt gaggatcagt ccgtcgatat tccatgagag cattgattcg
    78301 agacgttctt gctccatttc cggtttataa ccgtagtggg ccagcatggt ctgataaccg
    78361 tgcgcgtcgg tgacgctttc gattccgcgt aatacttccg cgaaaacctg gttggtgaga
    78421 gaaggtaaca ggacgccaat cgcccggctg gtggcgttag agaggatatc gggcgcacga
    78481 ttgggaatat agcccagttc atcaagagcc gcggcaatct tgccgcgtag agcgacggaa
    78541 acctgctccg ggttgcgtaa aaaacggctg accgtcattt tggtcacgcc tacacggtca
    78601 gccacatcct gaagtacggg tcttttcttt ttcatcgtcc tgaaggtaca aaagagatag
    78661 attctcttag tttaacacgg acgcatcaga accttcccgg ggaaaacagt gcttgtttac
    78721 aattattaga tatacactaa atcaattgca tcgcattgtg ctaacgacgc tgcaaaaccc
    78781 cggaagcgta catcagcacg cgaccggagc gagcgaggaa gcctgcaaaa aggcaggctt
    78841 caaagatgcc gttgagcttt aaaccggcgg cagatcgaac agtaacactt cgctgtcgct
    78901 atccgcatgg atggagattg cctgctcatc ccagattgcc agaccatcgc tgatcgaggc
    78961 tttcacgccg ttaatggtga cattgccttt caccacctgg atccagacgc ggcgttcagc
    79021 ggcaatctga tgcaccgact gctcatcttt cagcaacgcc cagcggtaca gttccatatc
    79081 ctgatgcact ttcaatgaac catcacgcgc atctggcgaa agcaccagtt gtttgccctg
    79141 cactgcatcg aagcgacgct gttcataacg cggcgtaatg ccgttttctt ccggcatgat
    79201 ccagatctga tacaggtgca gacgctcggt gctgcttggg ttgtactctg agtgacgaat
    79261 acccgtaccc gcactcataa tctggaactc gcccgccgga acctgctctt tattgcccat
    79321 gctgtcctgg tgctcaacag taccttccag cacgtaggtc aaaatttcca tatctttatg
    79381 cgggtgagtg ccgaagccct gccctgcttc aatcacgtcg tcgttaatca cgcgcagcgc
    79441 ggagaagccc ataaagttcg gatcgtaata gttggcaaaa gagaaagtat gccaggagtc
    79501 cagccagcca tgatttgcat gaccacgttc atttgctttg cgtaagtaga tcatttgtat
    79561 tcgccccctg aatgatttcg atgggcttag tgtggacccg atctgcctgg gatgatagag
    79621 ggtgaaaatt gacccctctg ttcaaaaaaa ttgaactatt cgaggggcca tcaaacttac
    79681 ttcgcgagag tcactgtgga gggagaggct tgctcgaatc cacgttcaag tatttccaga
    79741 ttcgtaagaa cttcagattc cttgacgtaa tttggcgcac cgtgggtgat ggtttgatac
    79801 aacgcatcat aaacgcgccc gtaatcgccc atctccggct tcatctcttc tctgaccgtc
    79861 acgccctcgt cattgacata ctccagcaca ccgaccgaat catccgctgc gaatcccggt
    79921 tcgcccggca taatattagc cttcaggctg gtttcctgct ggtcgatacc atatttaata
    79981 aacgaacctt tcttaccgtg aacgataaat ttcggataat cgattttcac cagatggctg
    80041 gttttgacga tggcttttag atcgccataa aacagttgcg cttcgaaagt atcgtcagga
    80101 ttggctttat tgcgcaggct gcggatgtca taagcgacgt gatccgggcg accgaacaga
    80161 gaaataatct ggtccatcgt atgcacacca aggccataga acgcgccatc ctgcggcagc
    80221 ccaggtttgg tttctgccac cgggcggtaa tagtcaaaat ggctttccac ttcgacaatc
    80281 tctcccagct tgccgctttc aatcgctttt ttcgccgtca ggaagcagga atcaaagcga
    80341 cgattctgat atggcgtgac ggtcagccct ttgcttttcg ccagtgcaaa cagctctttc
    80401 gcctgcgcaa gtgtcggagt gaacggtttt tcgaccagca catttttccc ggcttccatc
    80461 gcgcgcttcg cgtactcgaa gtggctgtcc gcatgggtgc agacaacaac cagcttaaca
    80521 tcgggatcgt ttagcacttc gttgagatcg ctggtgaaat ggatatgaga gtaaatggga
    80581 gcttgttctt ccggctttgc atggcggcga aaaatatgcg cgacatgcca gctatccttg
    80641 cggttaagta catacggcag atggtaacgg gtggtgcttt tgccgaagcc aataaaggcg
    80701 cagttgatga ccatgatttc gtccttttta aggtggttat ttacacctta gcgcaaagcg
    80761 gacgtggttc ctactggagt gcgcataacg aacacaagca cttcccgagg ataaattgag
    80821 aacgaaagat caaaaaaaaa gccagcaccc ggctggctaa gtaatactgg aagcaatgtg
    80881 agcaatgtcg tgctttcagg ttctccgcga gggtcttcct gatcgcgaga caataataat
    80941 cattctcatt cgcacttgtc caacactttt tgcaaaaaaa tgcatttgac tcgcattcga
    81001 aagtcaatga tgttgaaagg gacatttacc ccaaagagga caaaggaatg agtgagatag
    81061 tgatacgcca tgctgaaaca cgggattacg aggccatcag gcagattcac gcccagccgg
    81121 aggtgtatta caacacacta caggtgcctc atccttccga tcatatgtgg caggagcgac
    81181 tcgccgatcg tcccggcatc aagcaactcg tcgcctgtat tgatggagac gtcgtgggcc
    81241 atctcaccat tgacgtgcaa cagcgcccac gccgcagtca tgttgccgat tttggtatct
    81301 gtgtcgacgc ccgctggaag aatcgcggcg tcgccagcgc cctgatgcga gagatgattg
    81361 aaatgtgcga caactggttg cgggtagatc gtattgagct aacggtgttc gtcgataacg
    81421 cgccagcaat taaggtctat aaaaaattcg gctttgagat tgaagggact ggcaagaagt
    81481 acgcattacg taatggtgaa tatgttgatg cgtattttat ggcgcgggtg aagtaaagaa
    81541 tattctcaaa tt
//
LOCUS       DAZFTN010000008        80077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFTN010000008 DAZFTN010000000
VERSION     DAZFTN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(168..839)
                     /gene="queE"
                     /locus_tag="ACHKV2_000675"
     CDS             complement(168..839)
                     /gene="queE"
                     /locus_tag="ACHKV2_000675"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO6938612.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            994..1614
                     /locus_tag="ACHKV2_000676"
     CDS             994..1614
                     /locus_tag="ACHKV2_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO6938613.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1628..2302
                     /locus_tag="ACHKV2_000677"
     CDS             1628..2302
                     /locus_tag="ACHKV2_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6938614.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2536..3684
                     /locus_tag="ACHKV2_000678"
     CDS             2536..3684
                     /locus_tag="ACHKV2_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6938615.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            3681..4595
                     /locus_tag="ACHKV2_000679"
     CDS             3681..4595
                     /locus_tag="ACHKV2_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6938616.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4655..5953)
                     /gene="eno"
                     /locus_tag="ACHKV2_000680"
     CDS             complement(4655..5953)
                     /gene="eno"
                     /locus_tag="ACHKV2_000680"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO6938617.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6041..7678)
                     /gene="pyrG"
                     /locus_tag="ACHKV2_000681"
     CDS             complement(6041..7678)
                     /gene="pyrG"
                     /locus_tag="ACHKV2_000681"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO6938618.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7906..8697)
                     /gene="mazG"
                     /locus_tag="ACHKV2_000682"
     CDS             complement(7906..8697)
                     /gene="mazG"
                     /locus_tag="ACHKV2_000682"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO6938619.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8768..9103)
                     /gene="mazF"
                     /locus_tag="ACHKV2_000683"
     CDS             complement(8768..9103)
                     /gene="mazF"
                     /locus_tag="ACHKV2_000683"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO6938620.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9103..9351)
                     /gene="mazE"
                     /locus_tag="ACHKV2_000684"
     CDS             complement(9103..9351)
                     /gene="mazE"
                     /locus_tag="ACHKV2_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO6938621.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9429..11663)
                     /gene="relA"
                     /locus_tag="ACHKV2_000685"
     CDS             complement(9429..11663)
                     /gene="relA"
                     /locus_tag="ACHKV2_000685"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO6938622.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11711..13012)
                     /gene="rlmD"
                     /locus_tag="ACHKV2_000686"
     CDS             complement(11711..13012)
                     /gene="rlmD"
                     /locus_tag="ACHKV2_000686"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO6938623.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13069..15825
                     /gene="barA"
                     /locus_tag="ACHKV2_000687"
     CDS             13069..15825
                     /gene="barA"
                     /locus_tag="ACHKV2_000687"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO6938624.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16058..17398)
                     /gene="gudD"
                     /locus_tag="ACHKV2_000688"
     CDS             complement(16058..17398)
                     /gene="gudD"
                     /locus_tag="ACHKV2_000688"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO6938625.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17419..18759)
                     /gene="gudX"
                     /locus_tag="ACHKV2_000689"
                     /pseudo
     CDS             complement(17419..18759)
                     /gene="gudX"
                     /locus_tag="ACHKV2_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(18761..20113)
                     /gene="gudP"
                     /locus_tag="ACHKV2_000690"
     CDS             complement(18761..20113)
                     /gene="gudP"
                     /locus_tag="ACHKV2_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO6938626.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20548..20997)
                     /gene="yqcA"
                     /locus_tag="ACHKV2_000691"
     CDS             complement(20548..20997)
                     /gene="yqcA"
                     /locus_tag="ACHKV2_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO6938627.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21015..21797)
                     /gene="truC"
                     /locus_tag="ACHKV2_000692"
     CDS             complement(21015..21797)
                     /gene="truC"
                     /locus_tag="ACHKV2_000692"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO6938628.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(21797..22126)
                     /gene="yqcC"
                     /locus_tag="ACHKV2_000693"
     CDS             complement(21797..22126)
                     /gene="yqcC"
                     /locus_tag="ACHKV2_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO6938629.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22748..23293)
                     /gene="syd"
                     /locus_tag="ACHKV2_000694"
     CDS             complement(22748..23293)
                     /gene="syd"
                     /locus_tag="ACHKV2_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO6938630.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23361..24209
                     /gene="queF"
                     /locus_tag="ACHKV2_000695"
     CDS             23361..24209
                     /gene="queF"
                     /locus_tag="ACHKV2_000695"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO6938631.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24321..25685
                     /gene="ppnN"
                     /locus_tag="ACHKV2_000696"
     CDS             24321..25685
                     /gene="ppnN"
                     /locus_tag="ACHKV2_000696"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO6938632.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26242..27531
                     /gene="sdaC"
                     /locus_tag="ACHKV2_000697"
     CDS             26242..27531
                     /gene="sdaC"
                     /locus_tag="ACHKV2_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO6938633.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27589..28956
                     /gene="sdaB"
                     /locus_tag="ACHKV2_000698"
     CDS             27589..28956
                     /gene="sdaB"
                     /locus_tag="ACHKV2_000698"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO6938634.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            29068..29823
                     /gene="xni"
                     /locus_tag="ACHKV2_000699"
     CDS             29068..29823
                     /gene="xni"
                     /locus_tag="ACHKV2_000699"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO6938635.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(29878..31026)
                     /gene="fucO"
                     /locus_tag="ACHKV2_000700"
     CDS             complement(29878..31026)
                     /gene="fucO"
                     /locus_tag="ACHKV2_000700"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6938636.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(31054..31701)
                     /gene="fucA"
                     /locus_tag="ACHKV2_000701"
     CDS             complement(31054..31701)
                     /gene="fucA"
                     /locus_tag="ACHKV2_000701"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6938637.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            32248..33564
                     /gene="fucP"
                     /locus_tag="ACHKV2_000702"
     CDS             32248..33564
                     /gene="fucP"
                     /locus_tag="ACHKV2_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO6938638.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            33597..35372
                     /gene="fucI"
                     /locus_tag="ACHKV2_000703"
     CDS             33597..35372
                     /gene="fucI"
                     /locus_tag="ACHKV2_000703"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO6938639.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            35481..36899
                     /gene="fucK"
                     /locus_tag="ACHKV2_000704"
     CDS             35481..36899
                     /gene="fucK"
                     /locus_tag="ACHKV2_000704"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6938640.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQFRYFYPQTEPEFIEEV"
     gene            36901..37323
                     /gene="fucU"
                     /locus_tag="ACHKV2_000705"
     CDS             36901..37323
                     /gene="fucU"
                     /locus_tag="ACHKV2_000705"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO6938641.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            37381..38112
                     /gene="fucR"
                     /locus_tag="ACHKV2_000706"
     CDS             37381..38112
                     /gene="fucR"
                     /locus_tag="ACHKV2_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO6938642.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(38156..39256)
                     /gene="rlmM"
                     /locus_tag="ACHKV2_000707"
     CDS             complement(38156..39256)
                     /gene="rlmM"
                     /locus_tag="ACHKV2_000707"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO6938643.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(39249..39644)
                     /gene="ygdD"
                     /locus_tag="ACHKV2_000708"
     CDS             complement(39249..39644)
                     /gene="ygdD"
                     /locus_tag="ACHKV2_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO6938644.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(39663..40580)
                     /gene="gcvA"
                     /locus_tag="ACHKV2_000709"
     CDS             complement(39663..40580)
                     /gene="gcvA"
                     /locus_tag="ACHKV2_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO6938645.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(40931..41158)
                     /gene="ygdI"
                     /locus_tag="ACHKV2_000710"
     CDS             complement(40931..41158)
                     /gene="ygdI"
                     /locus_tag="ACHKV2_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO6938646.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            41350..42555
                     /gene="csdA"
                     /locus_tag="ACHKV2_000711"
     CDS             41350..42555
                     /gene="csdA"
                     /locus_tag="ACHKV2_000711"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO6938647.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            42555..42998
                     /gene="csdE"
                     /locus_tag="ACHKV2_000712"
     CDS             42555..42998
                     /gene="csdE"
                     /locus_tag="ACHKV2_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO6938648.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(43049..43855)
                     /gene="tcdA"
                     /locus_tag="ACHKV2_000713"
     CDS             complement(43049..43855)
                     /gene="tcdA"
                     /locus_tag="ACHKV2_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO6938649.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(44094..45191)
                     /gene="mltA"
                     /locus_tag="ACHKV2_000714"
     CDS             complement(44094..45191)
                     /gene="mltA"
                     /locus_tag="ACHKV2_000714"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO6938650.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            45400..45476
                     /locus_tag="ACHKV2_000715"
     tRNA            45400..45476
                     /locus_tag="ACHKV2_000715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45434..45436,aa:Met,seq:cat)
     gene            complement(45550..46803)
                     /gene="amiC"
                     /locus_tag="ACHKV2_000716"
     CDS             complement(45550..46803)
                     /gene="amiC"
                     /locus_tag="ACHKV2_000716"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO6938651.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            47035..48366
                     /gene="argA"
                     /locus_tag="ACHKV2_000717"
     CDS             47035..48366
                     /gene="argA"
                     /locus_tag="ACHKV2_000717"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO6938652.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(48428..50254)
                     /gene="recD"
                     /locus_tag="ACHKV2_000718"
     CDS             complement(48428..50254)
                     /gene="recD"
                     /locus_tag="ACHKV2_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO6938653.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(50254..53796)
                     /gene="recB"
                     /locus_tag="ACHKV2_000719"
     CDS             complement(50254..53796)
                     /gene="recB"
                     /locus_tag="ACHKV2_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6938654.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(53789..56677)
                     /gene="ptrA"
                     /locus_tag="ACHKV2_000720"
     CDS             complement(53789..56677)
                     /gene="ptrA"
                     /locus_tag="ACHKV2_000720"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO6938655.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(56853..60221)
                     /gene="recC"
                     /locus_tag="ACHKV2_000721"
     CDS             complement(56853..60221)
                     /gene="recC"
                     /locus_tag="ACHKV2_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO6938656.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDHELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(60234..60557)
                     /gene="ppdC"
                     /locus_tag="ACHKV2_000722"
     CDS             complement(60234..60557)
                     /gene="ppdC"
                     /locus_tag="ACHKV2_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO6938657.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(60542..60949)
                     /gene="ygdB"
                     /locus_tag="ACHKV2_000723"
     CDS             complement(60542..60949)
                     /gene="ygdB"
                     /locus_tag="ACHKV2_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO6938658.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(60946..61509)
                     /gene="ppdB"
                     /locus_tag="ACHKV2_000724"
     CDS             complement(60946..61509)
                     /gene="ppdB"
                     /locus_tag="ACHKV2_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6938659.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(61500..61970)
                     /gene="ppdA"
                     /locus_tag="ACHKV2_000725"
     CDS             complement(61500..61970)
                     /gene="ppdA"
                     /locus_tag="ACHKV2_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6938660.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(62154..62948)
                     /gene="thyA"
                     /locus_tag="ACHKV2_000726"
     CDS             complement(62154..62948)
                     /gene="thyA"
                     /locus_tag="ACHKV2_000726"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO6938661.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(62955..63830)
                     /gene="lgt"
                     /locus_tag="ACHKV2_000727"
     CDS             complement(62955..63830)
                     /gene="lgt"
                     /locus_tag="ACHKV2_000727"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO6938662.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(63981..66227)
                     /gene="ptsP"
                     /locus_tag="ACHKV2_000728"
     CDS             complement(63981..66227)
                     /gene="ptsP"
                     /locus_tag="ACHKV2_000728"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6938663.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(66240..66770)
                     /gene="rppH"
                     /locus_tag="ACHKV2_000729"
     CDS             complement(66240..66770)
                     /gene="rppH"
                     /locus_tag="ACHKV2_000729"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO6938664.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(67123..67269)
                     /gene="ygdT"
                     /locus_tag="ACHKV2_000730"
     CDS             complement(67123..67269)
                     /gene="ygdT"
                     /locus_tag="ACHKV2_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO6938665.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            67455..68144
                     /gene="mutH"
                     /locus_tag="ACHKV2_000731"
     CDS             67455..68144
                     /gene="mutH"
                     /locus_tag="ACHKV2_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO6938666.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            68213..68926
                     /gene="ygdQ"
                     /locus_tag="ACHKV2_000732"
     CDS             68213..68926
                     /gene="ygdQ"
                     /locus_tag="ACHKV2_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6938667.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            69064..69282
                     /gene="ygdR"
                     /locus_tag="ACHKV2_000733"
     CDS             69064..69282
                     /gene="ygdR"
                     /locus_tag="ACHKV2_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO6938668.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            69390..70430
                     /gene="tas"
                     /locus_tag="ACHKV2_000734"
     CDS             69390..70430
                     /gene="tas"
                     /locus_tag="ACHKV2_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO6938669.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(70462..71655)
                     /gene="lplT"
                     /locus_tag="ACHKV2_000735"
     CDS             complement(70462..71655)
                     /gene="lplT"
                     /locus_tag="ACHKV2_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO6938670.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(71648..73807)
                     /gene="aas"
                     /locus_tag="ACHKV2_000736"
     CDS             complement(71648..73807)
                     /gene="aas"
                     /locus_tag="ACHKV2_000736"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO6938671.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVHFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            74393..75424
                     /gene="galR"
                     /locus_tag="ACHKV2_000737"
     CDS             74393..75424
                     /gene="galR"
                     /locus_tag="ACHKV2_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO6938672.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(75431..76693)
                     /gene="lysA"
                     /locus_tag="ACHKV2_000738"
     CDS             complement(75431..76693)
                     /gene="lysA"
                     /locus_tag="ACHKV2_000738"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO6938673.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            76815..77750
                     /gene="lysR"
                     /locus_tag="ACHKV2_000739"
     CDS             76815..77750
                     /gene="lysR"
                     /locus_tag="ACHKV2_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO6938674.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(77737..78429)
                     /gene="ygeA"
                     /locus_tag="ACHKV2_000740"
     CDS             complement(77737..78429)
                     /gene="ygeA"
                     /locus_tag="ACHKV2_000740"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO6938675.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(78558..79976)
                     /gene="araE"
                     /locus_tag="ACHKV2_000741"
     CDS             complement(78558..79976)
                     /gene="araE"
                     /locus_tag="ACHKV2_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO6938676.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        19663 a        21441 c        19570 g        19403 t
ORIGIN      
        1 gcaataattt tcactccagc gaaaaattaa cgccacagaa tttgtagaga atgataatga
       61 ttagtccggg ataggcaaga tggatgagtt ttcagagacg acaaagaata taaatgagta
      121 gaagtcttta caggttccac ccatttataa cgcttataaa tgtttaatca ggcaatattt
      181 aaatatttat gggtttgcat cgacaaacgc caattacgcg caatgcaggt ttcaatgcac
      241 aaacgtgtgg catcatcctt ttggctaatc ggctgcagtg caatgactcg cggtttatca
      301 tcggtcagcg tcgccaaaag ttcatccagt gcttcaatat cacgtacgcg tcccactgga
      361 tgcttgattt cgttggctcg ctccagtgcc tgtgacaaca cttcatagcc gccgcgcatg
      421 ttcagctttg gcgatacggt aacccaggta ttcggtgtac agcgtacttc atgagtacca
      481 ctggtttcga tctggcagct aaaaccgttc ttttcgagca ggtcagtcag tggcagcaaa
      541 tcatgaatgc aaggctcacc acccgtaatc accacatgcc gcgcggtata tccctggcga
      601 ccaatgacag ccagcaagtc ttcactgctc gccgcccccc acttatcact ctctttggtc
      661 ttcgccagaa tgctgaaaag ggagacttcc cgatcctcaa gcttttccca ggtgtgtttg
      721 gtgtcgcacc aggcacagcc aaccgggcat ccctgtaaac gaataaaaat ggcggggacg
      781 ccggtaaagt aaccctcacc ttgcagggtc tggaacatct cgttaatcgg gtactgcata
      841 gcattctctg tgaagtggat aattgttaat tattgcagat cctgccacaa caatcatgtc
      901 ttattaacat tctgttacag gcaggtttat ggcaaaaagt ggattagatc ataaagaata
      961 aaatgaagca gagtattatg ttttaaggaa ttaattaaaa gggatttaaa cacgctcaga
     1021 atattcatcg taattatatt tattttcaat ttatcaggct gtggatataa cgatattcaa
     1081 acctatgatg agcaggttaa cgcctcctgg tctgaggtat tgaatcaata tcaacgtcgc
     1141 gccgatctta ttcctaatct tgtcgccagc attaaaggtt attccagcca tgaacgagat
     1201 gtactggaag ccgtaacatt ggctcgtagc caggctaatc gcgccagtag cgatcttcag
     1261 caaactccgg gagatgaaca aaaattacaa gcctggcaac aggctcaagc gcaggtgact
     1321 cgcactctcg gtcagttaac gataattagt gagcgttacc ctgagttaaa atctcaggaa
     1381 ctctatcaaa atttaatggt acagcttgaa ggaagtgaaa atcggattgc cgttgcgcga
     1441 ggaagatata ttaaagccat tgaacaatat aatgtcacta tccgcaaatt ccccgctgta
     1501 ttaaccgcaa aggtcatgga ttacacacca aagaaaaatt atttgccgga tgatgtcgcc
     1561 gcagtaagta aagccccaac aatagatttt agccagaatg ctaatgctca ttaagcgggg
     1621 aagttctatg cgaaatttta tcttccttat ggctttcttc tgttcatctg tgtttgccac
     1681 acaaattcca gtacctgaat cacccaagta tgtgaatgac ttaaccggca cattaacaaa
     1741 cagcgaagta aacaccctga ccaaccagat taaagccctg acgcaaaaaa actacgcaca
     1801 gttggtcgtg ttagtggttg aaacaacagg cgatgaaacc atcgagcagt acgcgacgcg
     1861 ggtattcgat agctggaaac cgggtgataa agaccgcgat gatggcgttc tactgttagt
     1921 cgcctggcag gatcataccg tgcgtattga aatcggttac ggactggaag gtattattac
     1981 ggatgcgcag tccgggaaaa ttattcgcaa cagtattatt cctgcattta aaaaaggcga
     2041 cctggctggt ggtttgcaaa agggaatcag cgatattgaa agtcgtctga cggggaataa
     2101 tttagcaacg ataacaccga ccgatcaccc tttgcccttt agcggttggt gggcattgtt
     2161 agtctgggcg atagtactga cgtttatttc tgccagagga tatatcaaaa cgctgggagt
     2221 aatttgcttc gcagctattg tactggcctt tgttttacct atagcaggct tcagcggaag
     2281 ctggggagta ctcgcaacat gactctgctt cgccacaccg ttcctggctg tcgccatcgc
     2341 ttttaccccc ttcggcaaaa aagttcggga tttgatgcgt gatgccaacc agccatcacg
     2401 ccatacaaga agcaattctt cctcccacta ttcatcatcg tcatcctctt cattcgacaa
     2461 tgacaatttt agcggtggtg gcggttcatc tggtggcggt ggcgcttccg ggcgctggta
     2521 acaaaaggaa ttgatatgcg tatttttctc ttacttctga tgctatgcgg ttttcaggta
     2581 ttcgctgaac gattgccttc agtgactgac ttaaccggga cattaacgac agaagaacaa
     2641 actgctttaa ctcaacaatt acagactctc gagcagcaaa accacttcca ggtggccgtg
     2701 ttggttacgc cgaccacagg gggcaaaaac attaaacttg cagcctcgca gagatacggg
     2761 gtatatcact ggaaacccgt tggagagaag cgatatggcg aaggtatctt aattctcgtc
     2821 gtatggccgg aagggctggc gtcaatgaag attggacatg gactggaaga gatgttgccg
     2881 ccggaacaag ctgcgcaaat tgttcgttac catatgcagc cagaattcga aaaaaacaat
     2941 ctctttgctg gattaacggg cggaattgaa agcatcgcac agtttaccca tatcaaagcg
     3001 aaccttagcc ctttggatgc tttagccaat catctgtttg ctaatccgca acgttcgctg
     3061 ccatgtcttg cctggacact gctgatgatc gttgccatcg ttgtcctctg gctatttgcc
     3121 agccgcccac ggcgtggcat atggatgata tcgatgatta cccctatggt ctggtttttc
     3181 tcctttcagg acgatgtcat cattcggcgg gtatcggtcg tttgttttag tttattgtta
     3241 gcaacaacct gcaagcagcg tctcgcggta gtttttaata tgtgccgcgt atttcctatg
     3301 atgctgaccc caaaaaataa aaacgctaaa gtgaaaaaac aaaagaagac tccgcaggtt
     3361 cgtgaatcag gacgaaattc attgtttgtc gtgatgctga taattattgt gggatggtgc
     3421 ctggtgttcg ccagtaataa agatattgca ttcatctcaa caattattgg gttaattgat
     3481 cattttatcg gcccaataac cattgctggc atcgttctgc tgatcattgt ggccaaattt
     3541 actggcaatc ttaagctggg gtcaggagaa aaaagcaaca ggaaaacggg gaatcgtaat
     3601 gctcattctc gctcttcgtc gagaaattct ttccgcggtg gtggcggttc ttcaggaggc
     3661 ggcggttcat ccgtccgttg gtgatcgtaa tgcgcttaat ctttattcta ttcactttgt
     3721 ggtgccttcc tgggcttgcc cagcaaattg ctgtgccgga actgcgccaa caggtgaccg
     3781 atattactgg tacgttaagc acttctgaac aacaatcact gacacagcaa ctgcaggata
     3841 ttacacataa aacacgggcg caggtcgctg tattagtagt gccgtccacc ggagacgatt
     3901 ccattgaaca atatgctaca agggtttttg atagctggaa actgggagat aagcagagaa
     3961 acgatggtat tttacttctg gttgcctggg aggatcatgc tgttcgtatt gaggttggct
     4021 acgggctgga aggggtggtt accgacctgc aagcggcgaa gattatcagg gatatattga
     4081 tccctgcctt caaaagtgat gacctgatgg gtggattaac actggcaagc gaaaatatcg
     4141 gcgcgcttct gttgaatggt gaattaccgg aagacagggg ggattattac agtatcaatc
     4201 cccctattcc attatcgctt gctgtaatta tattgctggc ggtactttca tattttattg
     4261 tttttaccga tccgtcgaat ttaccctgga tcactctcac tggcgccatt tacgggatgg
     4321 tattcctcta tgttgccgag ccaggaccat ggaccaattt aatcgttgcc tgcggtatgt
     4381 taacgccttt tgcgatcgta ccactggtta ttttttggct tatcgtcaat aaaaagctac
     4441 gcgccaaata caaaaaactc agtaaagata gagcttcaag aaaaggttct tcttcatcct
     4501 cttccggtgg cggctcaagt ggcggaggct caggcggcgg atttagcggc gggggcggtt
     4561 cttctggcgg tggcggcgcg tccggtcgct ggtaactcaa ccataaaaaa cgccagcccg
     4621 aaggctggca tttttaaatc agataaagtc agacttatgc ctggcctttg atctctttac
     4681 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
     4741 tagcaacgcg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
     4801 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
     4861 tgtagccagc gtctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
     4921 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
     4981 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
     5041 tctggtatgc gaaaccatcc cagtcagatt cgtccagacc gtcttcgatg gaaacgatcg
     5101 ggtactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aatgctttgt
     5161 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
     5221 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
     5281 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaacag
     5341 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
     5401 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
     5461 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
     5521 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atacctttag
     5581 cagctgcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     5641 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     5701 tagcatcttt gccaatcagc gcctgagcga tcgggccgtt taccgcagca acagctttgg
     5761 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     5821 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     5881 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     5941 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     6001 cgcgtaccca gggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     6061 ggaactcgct ggcggctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     6121 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     6181 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     6241 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     6301 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     6361 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     6421 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     6481 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     6541 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     6601 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcatccaga cctttaagga
     6661 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     6721 gattcttcag cccaccgtgt ttcagtgctt caatcacgga tttgtaagcg tccggcagtt
     6781 caatgtactt gccgaccata ccaatggtga cttcacttac cggattcgct tcttcgaaga
     6841 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     6901 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     6961 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     7021 cgttcgccgg aacggcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     7081 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     7141 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     7201 tctggcgaat cgcttcgagg aacggcaggg attcgatatc acctactgta ccgccgattt
     7261 cgaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     7321 tgatgtgcgg aataacctgt acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     7381 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgga
     7441 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     7501 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     7561 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     7621 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     7681 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     7741 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     7801 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     7861 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     7921 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     7981 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     8041 cgctttttgc aatgcgattt ccgcttttgt ccctaaatga cgagccagat tgaccgtggc
     8101 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     8161 ttcgtacatc acctcgtcga tctcttcgta gactttatcg actaccggac caagcgtcgt
     8221 ccaatcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcacgca ttaaagccgg
     8281 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     8341 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tgtcagcaaa
     8401 aacatgcgga tggcgacgct ctaatttatc gctaatcgca gcgcaaatat cattaaagtc
     8461 aaagcgccct tcttcctgag ccatttgggc gtaaaacacc acctggaaca gcagatcgcc
     8521 gagttcaccg cgcagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     8581 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     8641 gttttccggg tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattag
     8701 aatgtcctga aaattgcggg tccgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     8761 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     8821 caaccgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     8881 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     8941 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaacggac
     9001 tcaggacaac agccggacga tgtccggctt gctcgctacc ttttgtcggg tcaaaatcaa
     9061 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     9121 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     9181 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     9241 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     9301 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     9361 ctcaaaccac tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     9421 atttcggcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     9481 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     9541 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     9601 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     9661 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     9721 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     9781 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     9841 tggtgcatca ggttaccaac accttcaacc actacacgac cgttatcttt actgcggttt
     9901 tgcggcgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     9961 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
    10021 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
    10081 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
    10141 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
    10201 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
    10261 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
    10321 atgcgcccgc caatttttgc cccgatacag cggtgtccga catcactgtg gatgtggtaa
    10381 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt aggcgtaaag
    10441 acgtacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
    10501 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
    10561 gaacgtgcgc cgccagcagc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
    10621 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
    10681 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
    10741 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
    10801 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
    10861 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
    10921 tcagctttaa cgccttcagc tttcatctca gcgcgcagat gaccgacaaa ctcttcgata
    10981 tagtgttcgc ggtcgagacg gcgttcatgc aacagttttg cgatgcgttt gtattcggtc
    11041 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
    11101 cggtttgcca acggcgcgta gatattggtg cactcttttg ccgccagtac gcgttcatct
    11161 tccggcgcat cttttacttc acgcagatga gcaatacgct ccgccagttt gatgaccacg
    11221 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
    11281 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
    11341 tgaataaggt taacgaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
    11401 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
    11461 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
    11521 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
    11581 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
    11641 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
    11701 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
    11761 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
    11821 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
    11881 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
    11941 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
    12001 acgtcacatt ctgtaagcag ttaagacgcg cattctgctg gcctttttcc accagcgccg
    12061 gaacaccttc tacacccacc acactggcag cttgtgtcgc caatggtagt gtaaagttgc
    12121 ccataccgca gaacagatcc agtacgcgat cttcaggttc cacctccagc cattccaacg
    12181 cacgcgctac cattttttgg ttcacacccg cattaacctg aataaaatcg cgcgggctaa
    12241 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
    12301 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
    12361 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
    12421 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccaggcatg
    12481 ccctgacttt gggcagcaat gcttcaagtt ggggcactaa aatgggacat tgtttaacgt
    12541 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
    12601 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
    12661 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
    12721 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
    12781 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
    12841 tatcctcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
    12901 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
    12961 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
    13021 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
    13081 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
    13141 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
    13201 ggtgccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
    13261 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
    13321 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
    13381 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
    13441 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
    13501 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
    13561 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
    13621 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
    13681 ccgattcgca acatggtgaa tactgtcgac cgcatccgtc gcgggcaact cgacagccga
    13741 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
    13801 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
    13861 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
    13921 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
    13981 cgtacaccac tgaatggtgt tattggtttt acccgcctga cgctgaaaac agaattaaca
    14041 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
    14101 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
    14161 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
    14221 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
    14281 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
    14341 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
    14401 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    14461 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    14521 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    14581 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    14641 attattgaag ggccatccac ccagtgcctc gccggtaaac gcctggccta tgtcgaacca
    14701 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    14761 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    14821 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    14881 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc tgaaaaactc
    14941 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    15001 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    15061 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    15121 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    15181 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    15241 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    15301 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    15361 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    15421 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    15481 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    15541 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    15601 gaggaacagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    15661 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact tatcgaacaa
    15721 cagctacgta gcggtactaa agaagaagat ttggaaccgg agctgctgga actgttggac
    15781 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    15841 gtagcaatcg gctttttatg acgctgtttt cagattggtg acaaagtgcc tgtgtttatg
    15901 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca ataaattaaa
    15961 aagcatgtag gcctgataag cgtagcgcat caggcaattt tgtgtttggc atcagtctca
    16021 aaccggctcc agatagagcc ggttttggtt ttttgtctta acgcaccatg cacgggcgct
    16081 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    16141 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    16201 cacccagccc cggtttttct ggcacctgta ccagcccgcc tttgatctca aacggttctt
    16261 tggtcaggcg ctgattacct tcctgccaga tccagtgcgt atcaatagca gtaattttac
    16321 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgttag
    16381 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    16441 gcattgtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    16501 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    16561 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt
    16621 ctgcataagc cagcgaacct ttcaggtatt tgccgatttt aatcgcttcg ttcagcgacc
    16681 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    16741 tagactcggc ctcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    16801 catatttttc atacgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt
    16861 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    16921 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    16981 gaccatcgcc cagcagggat gccacgttaa cccccagatg ctgccccagc agatccagca
    17041 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    17101 cgcgcccgcc ggcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    17161 attcacccag cgttttaccc actaccagcg gaattgcatc ttccagcgtt ttacggattt
    17221 tctcgccgcc gggaatttcc cctacgccag tgtgaccaga attatctttg ataatcacaa
    17281 tattacgcgt gaagaacggt gcgtgtgcac cactcagatt catcagcata ctgtcatggc
    17341 ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat
    17401 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    17461 gccggggatc aggtactgca tcgggcctgc gtcgttacgc gcaccgccag gcagacgttt
    17521 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    17581 atcaggaacg gcaatttttc cgtttttaat ctccagcgga ttttgggtca ggcgacaatc
    17641 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    17701 cacatgggta aacatcgcca gagagatatc gaaatggttg ttggaatggc agccccaggt
    17761 cagcccccag tcgtcgcaaa gctgcgccac tcggactgca ccggaaagcg tccagaagtg
    17821 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    17881 gttggtggcg atcatattag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    17941 cacttcacgt ccggagaagc cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    18001 atcattcagc cctttgcaca gagaaatggc ttcatcaagc agccatgcac cgttgggatc
    18061 aacggtaatc cgcgcatccg ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    18121 ctcgccaggt aacacgccgc ccttaagttt gaaatcttta aagccgtagc gatcctgtga
    18181 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    18241 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    18301 gatataaaaa agataaccga ggacggtaat agcatcgcgt tgcttgcctg gccctaacag
    18361 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    18421 cgccacggcg ttaacgcgca attcaaaggt ccaggcccct ttaccgaagg tatcaaaatc
    18481 ggctgcttga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttccta
    18541 gcccagcacc atcggaatag catcgaccaa tgtctgataa atcacctctc cgcctggcgc
    18601 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gagtgaaata
    18661 tgcgttatgc gccccaccaa tattaagcaa catgctgtcg tacccggcga ccggaatgac
    18721 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc acgacaggtt
    18781 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    18841 catgaacacc aacataaatc agcgccccat taaacgagcc agtcgtgcca acgatataac
    18901 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    18961 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccaa
    19021 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    19081 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    19141 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataatccca cccagcacac
    19201 caccgataaa accacaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    19261 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    19321 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    19381 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    19441 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    19501 ttggctcgtg gatgactttc aaccagataa agctgatggc aatgcccaga ccgcccataa
    19561 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    19621 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    19681 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    19741 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    19801 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    19861 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    19921 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    19981 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    20041 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    20101 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    20161 gacatcgcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    20221 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    20281 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    20341 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    20401 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    20461 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    20521 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    20581 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    20641 aacccgctga gcgctctgtt cctgcagcaa ggcatcgaac tgtttgccgc cattgcagaa
    20701 attcacataa ctactgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    20761 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    20821 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    20881 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    20941 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    21001 gttttctccc tggattagga acttctctct ccatcctgac ccgatggcgc actgaactca
    21061 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    21121 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcaccag taaaaggatg cgtcaatgac
    21181 aactgactgg catgcagcat taaccgctga agaccaaaat gctcagcacc gctgcgattc
    21241 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    21301 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    21361 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    21421 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    21481 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    21541 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    21601 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    21661 tgctggccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    21721 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    21781 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    21841 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    21901 caacggcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    21961 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    22021 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    22081 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    22141 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    22201 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    22261 ttttcctctg gccatgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    22321 cccgatgtga tacttcatcc tgaagtgtcc caggccttcc tgacccaccg aatcatcctg
    22381 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    22441 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtccttcaga agtgtcatca
    22501 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    22561 gaactcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttagga
    22621 attaacaagc aacatgttgt ttttaaatga aaataacgat tcttatcttt ttaagtattc
    22681 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    22741 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt ggggctaaat
    22801 gcgtccgttt acgtgttccc agcgtctctt tacacacttc accggagaga ttacacaccg
    22861 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggtggc agttttagcc
    22921 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    22981 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    23041 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    23101 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcag
    23161 tagttgaaat aatgcacggt gaaggaacgc cgtacagttc ctcacttaac ggccagcttt
    23221 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    23281 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    23341 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    23401 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    23461 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcaaggta
    23521 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    23581 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    23641 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    23701 cgctggagcg cgacttaagc acttgcgctc agggtgaggt tagcgtggcg ttatatcgtc
    23761 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    23821 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagtggtg
    23881 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    23941 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    24001 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    24061 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    24121 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    24181 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    24241 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    24301 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    24361 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    24421 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    24481 tgctgtctcg ttttgaaaat ttcgatatta acgtcttgcg ccgtgaacgc ggcgtaaagc
    24541 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    24601 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    24661 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    24721 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    24781 ggggcggtca ctcaattaac gaaaatgagt atttgtatgc ccgtcgcgtc ggaaaccagc
    24841 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    24901 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    24961 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    25021 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    25081 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    25141 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    25201 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    25261 attaccgcat aatcattgat gacgccgctg aagtcgcccg tcagatgaaa aaatcgatgc
    25321 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    25381 gcattgcgcc agatttgcaa atgccatttg agccgtctca cgagaatatg gctaatctga
    25441 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    25501 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    25561 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    25621 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    25681 cgtaatctcc cctcccccgg tgacgtttgt tgccgggttt tctcgttttt ggtcacttac
    25741 tcatcaactc atttcatttg ttatatgaat gtttcttacc accctcacgc gacaaatatc
    25801 atcacagtta atatgtcata caatttatat tgcaacgcaa acgtttccct attttcataa
    25861 accgtatttt atcgctatga aaaagaaatt atcgccatga ttaactaaaa gtattgattt
    25921 tttcagttca acctacatat attgcgcgcc ccggaagaag tcagatgtcg tttaatgggc
    25981 aaatattgcc cttaaattct cttttacttt tgatttacag agtaaagcgt tgggataatc
    26041 tatcttccaa gtagattatt gtatttggga tcaagatcac tgatagatac ataacttgtg
    26101 tgtatctttc cgccctcaaa ttattacggc ggtaaatgat taagccatcg ccgatagaca
    26161 gatttcattt ttacggtcag gcaccttccc gggctgaact ggctaaaagc tgaattattt
    26221 gcattcctcc aggagaaata gatggaaacg actcaaacca gcacgattgc gtcgaaagac
    26281 tctcgtagtg cctggcgcaa gacagacacc atgtggatgc tgggccttta cggcacggca
    26341 atcggcgcgg gcgtgctgtt cctgccaatc aacgccggtg ttggcggtat gatcccgctg
    26401 atcatcatgg ctatccttgc gttcccgatg acgttttttg ctcaccgcgg cctgactcgc
    26461 ttcgtactgt ctggtaaaaa cccgggcgaa gacattaccg aggttgtaga agaacacttt
    26521 ggtattggcg caggtaaact gattaccctg ctctacttct tcgctatcta cccgatcctg
    26581 ctggtttata gcgtggcaat caccaataca gttgaaagct tcatgtctca ccagctgggt
    26641 atgacaccac cgccgcgtgc gattctgtcg ctgatcctga tcgtgggtat gatgaccatc
    26701 gttcgctttg gtgaacagat gatcgttaaa gcgatgagta ttctggtatt cccgtttgtt
    26761 ggcgtactga tgctgctggc tctgtacctg atcccgcagt ggaacggcgc agcactggaa
    26821 acgctgtctc tggacactgc atctgcaacc ggaaacggtc tgtggatgac cctgtggctg
    26881 gcaattccgg taatggtgtt ctcgttcaac cactctccga tcatctcttc tttcgccgtt
    26941 gcgaagcgtg aagagtacgg cgatatggca gaacagaaat gctcgaagat cctggcattc
    27001 gcacacatca tgatggtgct gaccgtaatg ttcttcgtct tcagctgcgt actgagcctg
    27061 actccggcag acctggctgc ggctaaagag cagaacatct cgattctgtc ttacctggct
    27121 aaccacttta acgcaccggt tatcgcgtgg atggctccga ttatcgcgat tatcgctatc
    27181 accaaatcct tcctcggcca ctacctgggc gcacgtgaag gcttcaacgg tatggtgatt
    27241 aaatctctgc gtggtaaagg taagtctatc gaaatcaaca agctgaaccg tatcactgcg
    27301 ctgttcatgc tggtaacgac ctggattgtt gccaccctga acccgagcat cctgggtatg
    27361 attgaaaccc tgggcggccc aatcatcgcg atgatcctgt tcctgatgcc gatgtacgca
    27421 attcagaaag taccggcaat gcgtaagtac agcggtcaca tcagcaacgt attcgttgtc
    27481 gtgatgggtc tgattgcaat ctccgcaatc ttctactctc tgttcagcta agtcctttcg
    27541 cgccgctttc gggcggcgct tcctccgttt taacgcgatg tatttcctat gattagcgta
    27601 ttcgatattt tcaaaatcgg cattggccct tccagttctc ataccgttgg accaatgaaa
    27661 gcgggtaaac aatttaccga cgatctgatt gcccgtaacc tgcttaaaga cgtgacccgc
    27721 gtggtggttg acgtgtacgg ctcgctctct ctgactggta aaggccacca cactgatatc
    27781 gccattatta tgggcctggc gggtaacctg ccggataccg tggatatcga ttccatccct
    27841 ggttttattc aggatgtgaa tactcatggt cgcctgatgc tggcaaacgg tcagcatgaa
    27901 gtggagttcc cggttgatca gtgcatgaac ttccatgccg acaacctttc tctgcatgaa
    27961 aacggtatgc gcattaccgc gctggcgggt gataaagtcg tttacagtca gacttactac
    28021 tctattggcg gtggctttat cgttgatgaa gagcattttg gccagcagga tagcgcaccg
    28081 gttgaagttc cttatccgta cagttcagca gccgatctgc aaaaacattg tcaggaaacc
    28141 gggctgtcac tctctggcct gatgatgaaa aacgagctgg cgctgcacag caaagaagag
    28201 ctggaacagc acctggcgaa cgtctgggaa gtgatgtgtg gcggtattga gcgcggtatt
    28261 tccaccgaag gtgtgttacc tggcaaactg cgtgttccac gccgtgctgc ggcactacgc
    28321 cggatgctgg tcagccagga taaaaccacc actgacccga tggcggttgt tgactggatc
    28381 aacatgtttg cactggcagt gaacgaagag aacgctgctg gcggtcgcgt ggtgactgcg
    28441 ccgactaacg gtgcgtgcgg gattatcccg gcagtgctgg cgtactacga taagtttatc
    28501 cgcgaagtga acgctaactc actggctcgt tacctgctgg tagccagcgc cattggttct
    28561 ctttataaga tgaacgcgtc gatttctggt gccgaagtgg gttgccaggg tgaagttggt
    28621 gtggcgtgct caatggcggc ggctggtctg gcagaactgt taggtgcaag cccggcgcag
    28681 gtgtgcatcg cggcggaaat cgccatggag cacaacctcg gtctgacgtg tgacccggtc
    28741 gccggacagg tacaggtgcc atgcatcgag cgtaacgcca ttgcggcagt aaaagcggtg
    28801 aacgccgcac gtatggcgct gcgccgtacc agcgagccgc gcgtctgcct cgataaagtt
    28861 atcgaaacca tgtacgaaac aggtaaagat atgaacgcca agtaccgcga aacctctcgc
    28921 ggcggcctgg caatgaagat cgttgcctgc gattaatcgc tctccagagg cctcgttttg
    28981 cgaggcctct tcccgatttc tcatccagcc atagcctgtt ccagtatcga atgttaccct
    29041 tatcgcctga tctttaaggg ggttatcgtg gctgttcatt tgcttattgt cgatgcactg
    29101 aatcttattc gtcgcattca tgccgttcag gggtcgccct gtgtcgaaac ctgccagcat
    29161 gcgctcgatc agctcattat gcacagccag ccaacccacg cggtcgccgt ttttgacgat
    29221 gaaaaccgca gtagcggctg gcgtcatcag cgtttaccag attacaaagc gggtcgaccg
    29281 ccaatgccgg aagagttgca cgacgagatg cctgcattac gcgccgcctt tgagcaacgc
    29341 ggcgtcccgt gctggtcaac cagcggcaac gaagccgatg acttagccgc cacgctggcg
    29401 gtcaaagtga cacaggccgg gcatcaggca acgattgttt cgacagataa aggctactgt
    29461 cagttacttt caccgacatt acgtattcgt gattacttcc agaaacgttg gctggatgcg
    29521 ccatttatcg ataaagaatt tggcgttcaa ccgcagcagt tgcccgatta ctggggactt
    29581 gcggggatca gcagttcaaa ggtaccgggt gttgcgggaa tcggaccaaa aagcgccacg
    29641 cagctgctgg tcgagtttca gagtctggaa gggatatatg agaatctgga tgcggttgcc
    29701 gaaaagtggc gcaaaaaatt agaaacccat aaagagatgg cgtttctgtg ccgcgatatt
    29761 gcccgcttac aaaccgattt gcatatcgac ggcaatttac agcaattgcg gttggtacgg
    29821 taacggcgtg ccggatacgc cgcaaacgtc gcatccggca ttatcacatc agcgcattta
    29881 ccaggcggta tggtaaagct ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc
    29941 ggtacaaaca tcatccagtg ccgcctgcgc cagtgccgga atgtcttcct tgcgtacacc
    30001 aacatcacgc aaatgtggcg gaataccgac atcacggttg agagcaaaca ccgcttcaac
    30061 agcggcatta cgcgcctctt ccaggctcat accttccact ttcacgccca taacgcgcgc
    30121 gatatcgcgg tacttctcac cggtaaagtc agcgttataa cgcatgacat gcggtaacag
    30181 gatggcgttt gcaacaccgt gtggagtgtt ataaaacgcg cccagtggat gcgccatacc
    30241 atgcaccaac cctaacccca cattcgagaa gcccataccc gcaacatact gcccgagcgc
    30301 catttcttct ccggcatcct tatcaccagc aaccgatcct cgcagcgccc cagcaatgat
    30361 ttcaatcgct ttaatgtgca gtgcatcggt tagcgcccac gcgccacggg taatataccc
    30421 ctcaatagca tgagtgagcg catcgacacc cgtcgcagct ttcagcgctg gaggcatacc
    30481 atccatcatg tcagcgtcaa taaacgccac ctgcgggata tcatgcggat caacgcaaac
    30541 aaacttgcgc cgtttttctt cgtcagtgat cacgtagtta atggtcactt ctgccgcagt
    30601 gcctgctgtg gtggggattg ccagaatcgg tacactgggt ttattggtcg gggaaagtcc
    30661 ttccaggcta cgcacatcgg caaactccgg gttgttgctg ataatgccaa tcgctttaca
    30721 agtatcctgt ggagaaccac caccaatagc gatcaggtaa tccgcgccgc tattctggaa
    30781 tacaccgagc ccttctttga cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat
    30841 cgcccatgcc agccctgcag catccatctt atcggtcact ttcgccacca cgccgcattg
    30901 caccagcgtt ttatcggtga cgatcagcgc cttctgataa ccacggcgtt tcacctcatc
    30961 ggttaaagcc ccaacagcac cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt
    31021 agccatcatc cttctccttg ttgctttacg aaattactct tcaatccgta acccataggt
    31081 tttgaatttc tccagcacta cggcaatctc ttcatcgctc agcactggca ccgggtccgt
    31141 aatcgccagg gtcgtcaggt aaagttgcgc cagcacttca acttcatgcg ccagccataa
    31201 cgctttttcc agattcacct cacaagcgat aagcccatga tgttgtaaca aagttgcctt
    31261 acgatttttg agagccagcg caacatgttc agaaagttcg cgtgttccaa aggtcgcata
    31321 aggcgcgcaa ggaatagaat taccgccagc cgccgcaatc atgtagtgaa tagcggggat
    31381 cggtcggtta agaatggaaa ctgctgtgca atgaacggca tgattgtgaa caaccgcgtt
    31441 ggcatccggt ctgctttgat aggccgccat atggaaacgc cactcgcttg aggggagctt
    31501 tccttcctca tgtttaccgt tgccatcaat aaagacaata tgcgactccg tcagtttttc
    31561 atatggaatg cctgtaggcg taatcagcat cccatcctga taacgtacac tgacgttccc
    31621 cgctgtcccc tggttcagtc ccaggcgggt catttccagg caagtgtcaa taatctgacg
    31681 agcaagttta tttcgttcca ttagctacct ctctctgatt caaaacaggg caataatgtt
    31741 gttcctttca cactattgaa ttagccgttt aattacccac catcttcttc ctgattaaca
    31801 agaaagaaat tcacaagctt atattttgtg acctagttca actaatcaca gtaaataact
    31861 gcaagttctc tttttataac cccattaaaa atgaccgctc ttaaaaatat ttatcaaaac
    31921 ggtcattttt ctattcaccc aagccaggaa tgcccgtttt cggcataaac aattaatacg
    31981 gtcatctggt ttgtgttttt tatgatttat tttctgaaac gggcatgaaa tttcgattat
    32041 taaagtgatg gtagtcacat aaagtcacct tccagctaat aagtgtgacc gccgtcatat
    32101 tacagagcgt tttttatttg aaaatgaatc catgagttca tttcagacag gcaaatattc
    32161 actgatatga agcccgaact cgctagtttt gcacttttga aaacataacc gattacgtgc
    32221 ttaagcttct gaacctaaga ggatgctatg ggaaacacat caatacaaac gcagagttac
    32281 cgtgcggtag ataaagatgc agggcaaagc agaagttaca ttattccatt cgcgctgctg
    32341 tgctcactgt tttttctttg ggcggtagcc aataacctta acgacatttt attacctcaa
    32401 ttccagcagg cttttacact gacaaatttc caggctggcc tgatccaatc ggccttttac
    32461 tttggttatt tcattatccc aattcctgct gggatattga tgaaaaaact cagttataaa
    32521 gcaggaatta ttaccggatt atttttatat gccttgggtg ctgcattatt ctggcccgcc
    32581 gcagaaataa tgaactacac cttattttta gttggcctat ttattattgc agccggatta
    32641 ggttgtctgg aaactgccgc aaaccctttt gttacggtat tagggccgga aagcagtggt
    32701 cacttccgct taaatcttgc gcaaacattt aactcatttg gcgcaattat cgcggttgtc
    32761 tttgggcaaa gtcttatttt gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa
    32821 atgtctccag agcaattgag tgcgtataaa cacagcctgg tattatcggt acagacacct
    32881 tatatgatca tcgtggctat cgtgttactg gtcgccctgc tgatcatgct gacgaaattc
    32941 ccggcattgc agagtgataa tcacagtgac gccaaacaag gatcgttctc cgcatcgctt
    33001 tctcgcctgg cgcgtattcg ccactggcgc tgggcagtat tagcgcaatt ctgctacgtt
    33061 ggcgcacaaa cggcctgctg gagctatttg attcgctacg ctgtagaaga aattccaggc
    33121 atgactgcag gctttgccgc taactattta accggaacca tggtgtgctt ctttattggt
    33181 cgtttcaccg gtacctggct catcagtcgc ttcgcaccac acaaagtcct ggccgcctac
    33241 gcattaatcg ctatggcact gtgcctgatc tcagccttcg ctggcagtca tgtgggctta
    33301 atagccctga ctttatgcag cgcctttatg tcgattcagt acccaacaat cttctcgctg
    33361 ggcattaaga atctcggcca ggacaccaaa tacggttcgt ccttcatcgt tatgaccatc
    33421 attggcggcg gtattgtcac tccggtcatg ggttttgtca gtgacgcggc gggcaacatc
    33481 cccactgctg aactgatccc cgcactctgc ttcgcggtca tctttatctt tgcccgtttc
    33541 cgttctcaaa cggcaactaa ctgaacatat tttccgaata aagtgaggaa tctgtaatga
    33601 acaaaatcag cttaccgaaa attggtatcc gcccggttat tgacggtcgt cgcatgggtg
    33661 ttcgtgagtc gcttgaagaa caaacaatga atatggcgaa agctacggcc gcactgctga
    33721 ccgagaaact gcgccatgcc tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta
    33781 tcgcgggtat ggctgaagcc gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc
    33841 tcaccattac ggtaacgcct tgctggtgct atggcagtga aaccatcgac atggatccaa
    33901 cccgcccgaa agccatttgg ggctttaacg gcactgaacg tcccggcgct gtttacctgg
    33961 cagcggctct ggcagctcac agccagaaag gcatcccggc attctccatt tacggtcatg
    34021 acgttcagga tgccgatgac acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct
    34081 ttgcccgcgc cggtttggcc gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg
    34141 gcgtttcgat gggtatcgcc ggttctattg ttgatcacaa cttctttgaa tcctggctgg
    34201 gaatgaaagt ccaggcggtg gatatgaccg aactgcgtcg ccgtatcgat cagaagattt
    34261 acgacgaagc cgaactggaa atggcactgg cctgggctga taaaaacttc cgctatggcg
    34321 aagatgaaaa taacaaacag tatcaacgca atgccgagca aagccgcgca gttctgcgcg
    34381 aaagtttact gatggcgatg tgtatccgcg acatgatgca aggcaacagc aaactggccg
    34441 atattggtcg cgtggaagaa tcacttggct acaacgccat cgctgcgggc ttccaggggc
    34501 aacgtcactg gaccgatcaa tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt
    34561 catttgactg gaatggcgtg cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta
    34621 acggcgtggc aatgctaatg ggtcaccagc tcaccggcac cgctcaggta tttgccgatg
    34681 tgcgtaccta ctggtcacca gaagcaattg agcgtgtaac ggggcataaa ctggatggac
    34741 tggcagaaca cggcatcatc catttgatca actccggttc tgctgcgctg gacggttcct
    34801 gtaaacaacg cgacagcgaa ggtaacccga cgatgaagcc gcactgggaa atctctcagc
    34861 aagaggctga cgcttgcctc gccgctaccg aatggtgccc ggcgatccac gaatacttcc
    34921 gtggcggcgg ttactcttcc cgcttcctta ccgaaggcgg cgtcccgttc accatgactc
    34981 gtgtcaacat catcaaaggc ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg
    35041 aattgccgaa ggatgtgcat gacatcctca acaaacgcac caactcaacc tggccaacca
    35101 cctggtttgc accgcgcctc accggtaaag ggccgtttac ggatgtgtac tcggtaatgg
    35161 cgaactgggg cgctaaccat ggggttctga ccatcggcca cgttggcgca gactttatca
    35221 ctctcgcctc catgctgcgt atcccggtat gtatgcacaa cgttgaagag accaaagtgt
    35281 atcgtccttc tgcctgggct gcgcacggca tggatattga aggccaggat taccgcgctt
    35341 gccagaacta cggtccgttg tacaagcgtt aatacattac tccctgatgt gatgcccggt
    35401 cgctccggct gccgggcctg aacaagcaag agtggttagc cggataagca atgttatccg
    35461 gctatattgc aggagcgatc atgaaacaag aagtgatcct ggtactcgac tgtggcgcga
    35521 ccaatgtcag ggccatcgcg gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc
    35581 ctaatgccag cgatatcgcg atggaaaaca acacctggca ccagtggtct ttagacgcca
    35641 ttttgcaacg ctttgctgat tgctgtcggc aaatcaatag tgaactgact gaatgccaca
    35701 tccgcggtat cgccgtcacc acctttggtg tggatggcgc tctggtagat aagcaaggca
    35761 atctgctcta tccgatcatt agctggaaat gtccgcgaac agcagcggtt atggacaata
    35821 ttgaacggtt aatctccgca cagcggttgc aggctatttc tggcgtcgga gcctttagtt
    35881 tcaatacgtt atataagttg gtgtggttga aagaaaatca tccacaactg ctggaacgcg
    35941 cgcacgcctg gctctttatt tcgtcgctga ttaaccaccg tttaaccggc gaattcacta
    36001 ctgatatcac gatggccgga accagccaga tgctggatat ccagcaacgc gatttcagtc
    36061 cgcaaatttt acaagccacc ggtattccac gccgactctt ccctcgtctg gtggaagcgg
    36121 gtgaacagat tggtacgcta cagaacagcg ccgcagcaat gctcggctta cccgttggca
    36181 taccggtgat ttccgcaggt cacgataccc agttcgccct ttttggcgct ggtgctgaac
    36241 aaaatgaacc cgtgctctct tccggtacat gggaaatttt aatggttcgc agcgcccagg
    36301 ttgatacttc gctgttaagt cagtacgccg gttccacctg cgaactggat agccaggcag
    36361 ggttgtataa cccaggtatg caatggctgg catccggtgt gctggaatgg gtgagaaaac
    36421 tgttctggac ggctgaaaca ccctggcaaa tgttgattga agaggctcgt ctgatcgcgc
    36481 ctggcgcgga tggcgtgaaa atgcagtgtg atttattgtc gtgtcagaac gctggctggc
    36541 aaggagtgac gcttaatacc acgcgggggc atttctatcg cgcggcgctg gaagggttaa
    36601 ctgcgcaatt acagcgcaat ctacagatgc tggaaaaaat cgggcacttt aaggcctctg
    36661 aattattgtt agtcggtgga ggaagtcgca acacattgtg gaatcagatt aaagccaata
    36721 tgcttgatat tccgttaaaa gttctcgacg acgccgaaac gaccgtcgca ggagctgcgc
    36781 tgttcggttg gtatggcgta ggggaattta acagcccgga agaagcccgt gcacagattc
    36841 attatcagtt ccgttatttc tacccgcaaa ctgaacctga atttatagag gaagtgtgaa
    36901 atgctgaaaa caatttcgcc gttaatttct cccgaactat tgaaagtgct ggcagagatg
    36961 ggacatggag atgaaattat tttttccgat gctcactttc ccgcccattc gatgggaccg
    37021 caggtgatcc gcgctgatgg cctgttggtg agcgacttgc tccaggcgat tatcccgtta
    37081 tttgagctgg acagttatgc accgccgctg gtgatgatgg cagcggtaga aggtgacact
    37141 ctcgatcctg aagtagaacg acgttaccgt aatgcgcttt cactacaagc cccgtgtcct
    37201 gacatcatcc gcatcaatcg ttttgcgttt tatgaacggg cgcaaaaagc ctttgcgatc
    37261 gttatcacag gcgaacgagc gaagtacggg aatattcttt taaaaaaagg ggtaacaccg
    37321 taatctcata ccggtacgcc cgcatgacgc gggcggttat cgaatgatgg ggtgaaaaat
    37381 atgaaagcgg cacgccagca agcgatagtc gacctgctgc tgaaccatac cagcctgacc
    37441 acggaagctc tctctgaaca gctaaaggtc agtaaagaaa ccattcgtcg cgatctcaat
    37501 gaattacaga cgcagggtaa aattctgcgc aatcatggac gcgctaaata tatccaccgt
    37561 caaaatcaag acagtggcga tccctttcac atcaggctga aaagccatta tgcgcataaa
    37621 gcagatatcg cgcgcgaggc gctcgcgtgg attgaagaag ggatggtgat agccttagac
    37681 gccagttcaa cttgctggta tctggcacgc cagttgcctg acatcaacat tcaggtcttc
    37741 accaatagcc atccgatttg ccatgaactc ggtaaacgcg aacgcattca actgatcagt
    37801 tccggcggca cacttgagcg caaatatggc tgttacgtca atccctcgct gatttcccaa
    37861 cttaaatcgc tggaaatcga tctgtttatt ttttcttgtg aagggatcga tagcagcggc
    37921 gcactgtggg actccaatgc tatcaacgct gattacaaat caatgctatt aaaacgcgcc
    37981 gcgcaatcgt tgttattgat tgataaaagt aaatttaatc gttcagggga agcccgcatc
    38041 gggcatctgg atgaggtaac gcacattatt tctgatgaac gccaggttgc aacttctttg
    38101 gtaacaggct gacggaaagg gtagcaggcc ggagacgacg ccccggcctt gcctgttatc
    38161 gctcgtcgcg acgaccaccc accgcagccc agatacggcg gacgtgcacc gtcacttctt
    38221 cgcgatcgtg atacaactgc cgcgcctgaa tctgagcatt tatgccatgt tcatcaagct
    38281 gtgcctgaat atacgccaga ttgtgtgaca cttcttcgta gcgttttttc atcggcagtt
    38341 tgaggttgaa aatggtttca cgacaccagc cattaaccag ccactgcgcc atcaatgccg
    38401 caactttcgc cggtttttca accatatcgc ataccatcca ggagatattg ctgcgcgtcg
    38461 gacggaattt gaaaccgtct tcccgcagcc acgtcacctg tccggtatcc atcagacttt
    38521 gcgccatcgg gccgttgtcg acggaataaa cccacatgtt gcgcttcacc agttggtagg
    38581 tccagccgcc agggcaagcg cctaaatcca ccgcccacat cccgttcgcc aggcgttcat
    38641 cccactcatc cgcaggaata aacacatgaa atgcctcttc cagtttgagc gtggaacgac
    38701 tcggcgcatc tgccggaaat ttcaggcgcg gaatgcccat atagaacggc gaattattgt
    38761 tgctgtatga gtaaccggta tagcagcagc ctggtgcaat aaagaataca tgcacaaccg
    38821 gacgcttcgg cgtttcatag ttcgccagca ccccggcatc gcgcaaggca gcgcgtagcg
    38881 gcacggtgaa tttacggcag aatttcagca actctttgct ttcgttggta tcggcaactt
    38941 caacacgcag ttcaccgccc ttctctacta cgccctgcaa catgccgaca atgggggtaa
    39001 tacgatcttc tggcggcaaa tgctgcaaga gttcccccac cacaaaccac tggcgggcaa
    39061 aaattaatga actgaacggc agctcacgga ttaacttatc gccatcatca ggttgataac
    39121 attcataaat gacataaccc gcattctctt tcacgcgggc aaaaccgaaa atctcccgct
    39181 ggccggcttt atcggtaatt tctgcggcgc actctttttc aaagcccgga cggcacagca
    39241 atacaacctt attcatgact tacgcccttg cgctttaaac ggatagcacc aactaacatt
    39301 aacgcccagc ccgcaaggaa gctcacgccg ccaaccggag tgacaaacgc ccacaaacgc
    39361 agatgggaca gtgccaggca ataaaggctg ccgctgaaca gcaccgtgcc taacgcgagg
    39421 aaaacgctac tccagtaaaa ccagatactg atgcgacgct gcattgccac cgccagacct
    39481 aagatcgcca gcgtatgaaa cgcctggtat tcgaggccgg tctggatcca gcccatctca
    39541 acggccccca tggttttact taacacatgc gcgccaaaag cgcccagtgc cacaaaaatg
    39601 aagccgctaa tggcggcgaa aatcagcata aaacggctgg tcatggtcgt accctacgta
    39661 aattattgtt cataacgaaa gcggaatttt tcttgttcag cagcggcttt cgccaggatc
    39721 cattggcgaa aggcggctat tttacccagt tctgcctggc tgtcatgaca aaccagataa
    39781 aaagcgtttt tactgaccag aacatcatta aacgggcaaa caagacgtcc ggcttcaatt
    39841 tcagattgcg ccatcacgtt atttgccagc gccactccct gcccgtgaat agccgcttgc
    39901 agcaccatgg cgctatggct aaaaattggc ccttgctgaa cgttgatatg atttaacccc
    39961 aattgacgag tatatgtctg ccagtcacgg cgtgacgcat catgtaataa cgtatgttta
    40021 gccagatctt ccggggtctt caagggtttt tcgccagtca gcagtagcgg cgaacacacc
    40081 ggcaataaat attcggcgta cagtttttcc acccgtagcc ccggccagtt gccccgacca
    40141 taaaatatcg ccacatcaac atcatccgcc agcttatctt cctgacgatc aaccgcctgg
    40201 attcgaacgt caattcccgg ataagctgaa ttaaagctgg aaagtcgcgg aaccaaccaa
    40261 tgaatggcga aactggggag taaactgacc gtcaacgccc ccttggcgct acgggcctgg
    40321 agtttacgcg tcgcttcggt taattgcgaa aatatctctt tgatatcgag gaaatagctt
    40381 tgcccttcct cggtcagcag gagtgaacga ttacggcggc ggaacagttt tagccccaaa
    40441 aaatcctcaa gagacttgat ttgatgactt actgcggcct gggtcacaaa aagctcttct
    40501 gctgcgcgag tgaaacttaa atggcgtgct gcggcatcaa aaactcgtaa ggcatttagc
    40561 ggtggtaatc gtttagacat ggcttttaaa ctttgatgtt aaatgaattt aacaattaga
    40621 tcacactgtg taacctatta gtttttttaa tctgagccat tataaattgt ccgttgagct
    40681 tctaccagca aatacctata gtggcggcac ttcctgagcc ggaacgaaaa gttttatcgg
    40741 aatgcgtgtt ctggtgaact tttggcttac ggttgtgatg ttgtgttgtt gtgtttgcaa
    40801 ttggtctgcg attcagacca tggtagcaaa gctacctttt ttcacttcct gtacatttac
    40861 cctgtctgtc catagtgatt aatgtagcac cgcctaattg cggtgctttt ttttaccttg
    40921 cgatcgcgaa ttactgatcc agttcgacca tctctttcac gtcggtacgg ttgatctgct
    40981 gtttgttgcc attagcgtct ttatacgaaa tcataccggt atcgttatca gtctgtggtt
    41041 tgccgtcaga gacgatggta cgtccgtcat tggtgtgcat cacatagttc gaaccggaac
    41101 aggcgctcag ggcaaaagtc agcatacagg cagaaataat tgcggcagtc tttttcatca
    41161 tcttctcctt aaagcgatta ataaatctta atagtattga ttaacaggct aaaattaacg
    41221 cctaacacta ttcagcatat gttactgacg cggcttcgcc aggatatcca gataattctg
    41281 atggttagca ctctccttgt atcaaagtga attttgcgtc acgatcggtg catcaagccg
    41341 aggagtacca tgaacgtttt taatcccgcg cagtttcgcg cccagtttcc cgctctacag
    41401 gatgcgggcg tctatctcga cagcgccgcg accgcgctta aacctgaagc cgtggttgaa
    41461 gccacccgac agttttacag tctgagcgcc ggaaacgtcc atcgcagcca gtttgccgaa
    41521 gcccaacgcc tgaccgcgcg ttatgaagct gcacgagaga aagtggcgca attactgaat
    41581 gcaccggatg ataaaactat cgtctggacg cgcggcacca ctgaatccat caacatggtg
    41641 gcacaatgct atgcgcgtcc gcgtctacaa ccgggcgatg agattattgt cagcgtggca
    41701 gaacaccacg ccaacctcgt cccctggctg atggtcgccc aacaaactgg agccaaagtg
    41761 gtcaaattgc cgcttaatgc gcagcgactg ccagatgtcg atttgctgcc ggaactgatt
    41821 actccccgta gtcggattct ggcgttgggt cagatgtcga acgtaactgg cggttgcccg
    41881 gatctggcgc gagcgattac ctttgctcat tcagccggga tagtggtgat agttgatggt
    41941 gctcaggggg cggtgcattt ccccgcggat gttcagcaac tggatatcga tttctatgct
    42001 ttctctggtc acaaactgta tggcccgacg ggtatcggcg tgctgtatgg caaatcagaa
    42061 ctgctggaag cgatgtcgcc ctggctgggc ggcggcaaaa tggttcacga agtgagtttt
    42121 gacggcttca cgactcaatc tgcgccgtgg aaactggaag caggaacgcc aaatgtggct
    42181 ggtgtcatag gattaagcgc ggcgctggaa tggctggcag attacgatat caaccaggcc
    42241 gaaaactgga gccgtagctt agcaacgctg gcggaagatg cgctggcgaa acgtccaggc
    42301 tttcgttcat tccgctgcca ggattccagc ctgctggcct ttgattttgc tggcgttcat
    42361 cacagcgata tggtgacgct gctggcggag tacggtattg ccttgcgggc cgggcaacat
    42421 tgcgctcagc cgctactggc agaattaggc gtgaccggca cactgcgcgc ctcttttgcg
    42481 ccatataata caaagagtga tgtggatgcg ctggtgaatg ccgttgaccg cgcgctggaa
    42541 ttattggtgg attaatgacg aacccgcaat tcgccggaca tccgttcggc acaaccgtaa
    42601 ccgcagaaac gttacgcaat accttcgcac cgttgacgca atgggaagat aaatatcgcc
    42661 agttgatcat gctggggaaa cagcttccgg ctttgccaga cgagttaaaa gcgcaggcta
    42721 aagagattgc cggatgcgaa aaccgcgtct ggctgggata taccgtggct gaaaacggta
    42781 aaatgcactt ctttggcgac agcgaaggac gtatagtgcg cggcctcctg gcggtgctgt
    42841 tgactgccgt tgaggggaaa accgccgccg aattgcaggc acagtcgccg ctggcattgt
    42901 ttgatgagct gggattgcgt gcgcaactta gcgcctcacg cagccagggg ttaaatgcgt
    42961 taagcgaggc gattatcgct gcgacgaagc aggtttaagc acagaaccgg gtcggataag
    43021 acgtttgcgt cgcatccgac ctgattgttt acccctgacg cgccgctttc gccatcatct
    43081 tcttcagcgc atgagaaacc gcaacaaaac caaaggtggc ggtcaccatc gttgccgcgc
    43141 caaatccaga tgcacaatcc atccgctttg gtccttccgc cgtggctttc atcgcacaca
    43201 ccgtaccgtc tgactgcgga tacaccaatg cttcggtaga gaacacgcaa tccacaccga
    43261 gcttaccttt actgtttttc actacgccaa aatcgctttt caggcgctca cgcaacttcg
    43321 ccgccagcgg gtcctgaatc gttttcgcca gatcggtaac ctgaatctgc gttgggtcaa
    43381 tctgcccacc cgcgccaccg gtcgtgacca gcgggatttt attgcgccga caataagcaa
    43441 tcagcgccgc tttgggccgt acactatcaa tggcatcaat cacgtacgaa taacccacgc
    43501 tcatatgctg cgctacgtta tccggcgtca cgaaatcatc caccaccgtt acacagcact
    43561 ccgagttaat ctggcgaata cgttccgcca tgacttccgc ttttgccagc ccaacgttat
    43621 cgcgcagggc atgaatttgc cgattggtat tggtgacgca cacatcatcc atatcgataa
    43681 gcgtgattgc gccaatcccc gtgcgcgcca gcgcttccgc cgcccaggaa ccgacaccgc
    43741 caataccgac cacacaaata tgcgcgtcag caaacagttg caacgctttt tcaccataca
    43801 gacgcgctgt gccaccaaaa cgctgacgcc atgcatcact aattaccaca gacatagcac
    43861 ctcagaatta aaaagggcga ggttatccct caccctaaac gcataaatgc ctgatgcgct
    43921 acgcttatca ggcctacata actcctgcaa tattttggat ttgcatgatt tgtaggccgg
    43981 ataaggcgtt cacgccgcat ccggcatgaa caacgcgcac ttagtcaata atcaggatgt
    44041 gaggttaccc ctcaccctgt catatccgta aaaacggcat acagaatatc acatcagccg
    44101 ctaaagacgt tacctgcgcc cggggcggtt ttcagcaccc agacacgtcc atagtggttg
    44161 taccaacctg cgcggtgtcc ggcttccggc ccgatccctt gataaatatc gaagtgttgg
    44221 cctttgattg cgccaccgac atccaacgcc accatcagac gcaattcgta ctgaccgtta
    44281 aatttgccgt tattatccag cagcggcact tctgccagca aggttgtacc tggtgggata
    44341 atggaacgat cagaggcaac agaagcacgg ccaaccagcg gcaccgcact tgcccctttc
    44401 accggtgcaa aagattgcgg tttaaagaaa acgaaagacg ggttctgttc cagcagctcg
    44461 cgaacctcgg cttcactgtg tgtttcgccc caatgacgaa tcgcctgcat cgacatatct
    44521 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
    44581 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatataccca
    44641 ctaccctgca catccataat gaaattatcc atcagagagt tgctgtaagc gaggatgtac
    44701 ttatcgctca atgcacccgc gtagatctcc gcacgagacg gcagacgacc acgttttggc
    44761 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
    44821 ggcgtgtaat agcctgtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
    44881 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
    44941 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
    45001 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctggttc
    45061 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
    45121 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
    45181 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
    45241 accgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct
    45301 ttagtgattt gtttttgttc aaaatcatgc cagatccgtg atcggggtaa aaaaaaggtt
    45361 gcatgaaaac gcgagcggag tatagtgcgc atccacggac gcggggtgga gcagcctggt
    45421 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatta
    45481 ttgaacaccc taacgggtgt ttttttgttt ctggtctcct ataaaaaagc gccattcagc
    45541 gcctttttat catccccttc tcgccagggt cgccccatcg gcaaaatacg ctttaattcc
    45601 cgcaagaata gactccgcaa cttcctgctg gaaggtcgcc gttttcagtt tacgctcttc
    45661 ctcaacgtta ctgataaacg ccgtttcgac cagaatggag ggaatatctg gtgcctttag
    45721 tacggcaaac ccggcctgtt caacttgatt tttatgcagc ttgttgattt taccgagctt
    45781 attcagcacc gctttaccaa acttaaggct gtcagcaatg gtcagcgact gcaccatatc
    45841 gaacatggtg tggtcgacat agcggtcacc gcttttgctc acgccaccaa tcaagtcgga
    45901 ggcgttctgg gtttgtgcca gatattttgc cgcagtactg gttgcacctt tggttgagag
    45961 cgcaaacacc gaggaaccgc tcggctggcg actggtaaag gcgtcggcat ggatagagac
    46021 aaacaggtca gcacgctgtt tctgagcttt tgctacgcgc acttgcaacg gaatgaagat
    46081 gtcttcattg cgcgtcatgt acaccttcat attgccctct ttctcgatca gagagcgcaa
    46141 acggcgagct atttgcaata ccacatcttt ttcgcgcgtt ttgtatttcc ccaccgcacc
    46201 ggagtcttcg ccaccgtggc cagggtcaag cataatgaca atcggacgat cgcgccctgc
    46261 tttacctggt tgtggaccac tttgcgctgg cggcacctgc ttttcgaggt cgcctttgtt
    46321 gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc tgtgcattgg ccggatagag
    46381 gtccatcacc agacgctctt taaacccggc gaccggcgca agggcaaaca gctgcggttt
    46441 tacgttttgc tttaattcaa aaaccatccg tacggtttgc gggtcaaatt gcccgacgcg
    46501 cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc gccatcccct tgagtaccga
    46561 gttcaggttt acatcttcga tatccaccac cacgcgttcc gggttactca acgcgaactg
    46621 cttatatttc agctgacgat ttgattctac cgtcacgcgg gtgtagctgg acgcaggcca
    46681 gacgcgcacc gccacgacct ggctgaccgc agccagactg acctgactta cgctcaatag
    46741 ccacatggca cccgcgcctt gcagtaaacg acgacggctg attgcagtgt tggatcctga
    46801 catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca agtaaacgac taatttgacc
    46861 ggaaacttta gcgaaagacg cataatctgt catctaataa acggtaaaca ttctttttat
    46921 attcacgcca ttactgataa aaaagtcgct ctcgcataaa atttacactt gcaccctgcg
    46981 aaaaaacaga ataaaaatac aataaattcg aataataatg caaagaggtg tgccgtggta
    47041 aaggaacgta aaaccgagct ggtcgaggga ttccgccatt cggttcccta tatcaatacc
    47101 caccggggaa aaacgtttgt catcatgctc ggcggtgaag ccattgagca tgagaatttc
    47161 tccagtatcg ttaatgatat cgggttgttg cacagcctcg gcatccgtct ggtggtggtc
    47221 tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc atcaccacga accgctgtat
    47281 cacaagaata tacgtgtgac cgacgccaaa acactggaac tggtgaagca ggctgcggga
    47341 acattgcaac tggatattac tgctcgcctg tcgatgagtc tcaataacac gccgctacag
    47401 ggcgcgcata tcaacgtcgt cagtggcaat tttattattg cccagccgct gggcgtcgat
    47461 gacggcgtgg attactgcca tagcgggcgt atccggcgga ttgatgaaga cgcgatccat
    47521 cgtcaactgg acagcggtgc aatagtgcta atggggccgg tcgctgtttc agtcactggc
    47581 gagagcttta atctgacctc ggaagagatt gccactcaac tggccatcaa actgaaagct
    47641 gagaagatga ttggtttttg ctcttcccag ggcgtcacta atgacgacgg tgatattgtc
    47701 tccgaacttt tccctaacga agcgcaagcg cgggtagaag cccaggaaga gaaaggcgat
    47761 tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg
    47821 cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc tgttgcaaga gttgttctca
    47881 cgcgacggta tcggtacgca gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca
    47941 atcaacgata tcggcggtat tctggagttg attcgcccgc tggagcagca aggtattctg
    48001 gtacgccgtt ctcgcgagca gctggagatg gaaatcgaca aattcaccat tattcagcgc
    48061 gataacacga ctattgcctg cgccgcgctc tatccgttcc cggaagagaa gattggggaa
    48121 atggcctgtg tggcagttca cccggattac cgcagctcat cacggggcga ggttctgctg
    48181 gaacgcattg ccgctcaggc gaagcagagc ggcttaagca aattgtttgt gctgaccacg
    48241 cgcagtattc actggttcca ggaacgtgga tttaccccgg tggatattga tttattgccc
    48301 gaaagcaaaa agcagttgta caactaccag cgtaaatcca aagtgttgat ggcggattta
    48361 gggtaagcga tgaaaatcgt cggatgcgac atgcgtaaca ctcgtacgtc gcatccggca
    48421 attacgttta ttcccgtgaa ctaaacaacg ccgccagacc actgcgccgc tcagtacgag
    48481 tggcgattgc cgcacttaat atgcgctcat cggcatacag cgacagacgg cgacgcgcgc
    48541 gggtcaccgc ggtataaacc agctctcgcg ttactaccgg cgtgcgttgg ctcggtaaaa
    48601 tcaacgccgc atggtcgaac tccgatccct gcgatttatg taccgtcatc gcccacgtag
    48661 tttcgtgctc tggcaagcga ctcggttgca cagacttaat attgccgtcc ggcatcgcaa
    48721 accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca ttaaacaacc
    48781 caagcgcgct gtcattacgg gcaatcatca ccggtcggcc ttcgtaccaa cgagagtgcg
    48841 gattacgatg aattttgcgc ttctgttgca taaactgctc aattcgctca ttcagtccag
    48901 ccacgccaaa tggcccttcc cgcagggcgc acaaaagttg gtactcattg aacgcctgaa
    48961 tgattaaatc cggctcggca cgcgcttgca gcagatccag ataacgtcca taacccgcaa
    49021 gagcttcctc aagcatcgca atataatctt cgccgctctg taaaagccgt ttttcaatat
    49081 cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt atcaccacgg ttgatcgccg
    49141 cagctaactg accaatgcca gaatcgctgc cgaaacgata gcttttttgc agcaggcaga
    49201 gactgtcgcg caaagatgcc gcttctgtgc cagttcctgc cggaacgtga gttcccgtca
    49261 ggcggcttag ctgcccggca cgctcggcgg taaagccggc attggcataa gcgcagatat
    49321 cgcccagcac agccccagcc tcaaccgagg ccagttgatc acgatcgccg agaaagatca
    49381 ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat cataggcaga tcgatcattg
    49441 acgcttcatc taccaccagc acatcaagat gcagcgggtt accggcatga tgacgtaaac
    49501 gctggctacc cggctgcgca cccagcaatc ggtgcaaagt actggcatct tccggaatac
    49561 gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc cttgccgaga gattcggtta
    49621 agcgcgcggc agctttaccc gttggtgcag ccagacggat acggcagcgt tcgccgtcgg
    49681 ccatttgaat taacgctgcc agcaacttcg ctacggtggt cgttttaccg gtgccagggc
    49741 cgccggaaat caccgagatc cgccgcgtca gcgccactgc tgccgccact ttttgccagt
    49801 taatttcatc accagtcgga aagagtttat ccagggtttg cgccaacaag gcttcatcca
    49861 cctcgatggc atgattcacc tcattgaaga agcgcgctac cgtccgctcg ttacaccaca
    49921 tgcgattcaa ataaagacga tcgccacaga ggatcatcgg cgtgggttca tctccccggc
    49981 tgaccgcttg tgaagccagc aagcattctt cccaattttg tagctcaccg atttcactga
    50041 cacaggtcgc caacagcgga tgcgactcct cattattttc cagtcgtgaa agcggcaaac
    50101 aaacgtgccc ctctccggca tcatgactta acagtgccgc cgcgagggtg acggcaggat
    50161 gttcatctcc cgccacggtc agggcaaact gcacgtccag cgggcgtagc tgtttgtgct
    50221 ccacagcttc cagtaattgc ttttgcaatt tcattacgcc tcctccagag tcataccggc
    50281 aaacatctca tccatcaggt caatcaaccc ggcgttgggg cgggttgtgt aaatcccttg
    50341 ttgcggatgt tctttatcaa caccacgcag gaacagataa ataacgccgc caaagtggcg
    50401 ctcatagtcg taatcagcaa tgcgatggcg cagataacgg tgcagcgcca gggtataaag
    50461 ctgatattgc agatcatagc ggtgtgcctg cattgccgct gccatagcct gttgggtgta
    50521 agccgaacta tcttcaccca accagttgga tttatagtcg agcaggtaat aacgcccttc
    50581 gtggcggaac accaggtcga taaagccttt taacatgcca cgtgcctgca tgaactccag
    50641 cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt agtgcatcaa gctggctggc
    50701 gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc acctgtttat cgcggtcgga
    50761 aagctgactc aggctaacac ccgtttcatt gagaggtgcc tggaggacag ccgtgatcca
    50821 ctcggtcaac accggttccc actgcgattc aaagccgccg agttccagtt tttcctgtac
    50881 ccagttcggg tcaaccggct gggtaaaatt gaggtcttca aacaaactgt gcaagaatgt
    50941 ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac gtcggttctt caacgacgct
    51001 gaccacgcct gcggcatcaa catccagccg tggcatcaga tcctgagcga taccgtgacc
    51061 acgctgctgc aagccggagt agctggtgac acgccagtta tcgccgggca atcgttgtag
    51121 cgtcctcgca ttcagttctg cagttaaagc atcattaacc tgccagggct ggttatcacc
    51181 agtttgtgcc gtttgccagg caatatcatc atcgcataac gcttcaatac aggtgcgaag
    51241 ccctgccgca tcttgcggtt cccctttttg cagcaaacgc ccgagggcac tttggtggac
    51301 gtcggtgtca ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc cgagactgca
    51361 atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc agatcttccg ccagacgttc
    51421 gacctccgca agatcgacgc tttctggcgc agcgttaaga tccagaacag cctcaaacga
    51481 gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa ttggtaataa atggcaacca
    51541 gaccagcgga tattccagcc cttttgattt atggatcgtg acaatctgta ccagatgttt
    51601 atcactttcg aggcgcattt gttggctgga ggcattactg tctggctcga ggatatgttg
    51661 cgataaccag cgtaccagcg catgttcact ttccagctgc gttccggctt cttgtagcag
    51721 ttcgctgata tgcaagatat cggtaagacg ccgctcaccg cctgccgttg ccagcaagtt
    51781 ttctgcaatg ttacgcgccg acatcagcgc ccgcatcatc ggcataacgc cacgtttgcg
    51841 ccagatttgc cgataaccat cgaactcttc gaccaccgcg tcccacgcat gttcgtcatt
    51901 attcagcgtt tcgatatcca gcgcgttcag ccccatcatt gacgttgcca gcgcactacg
    51961 cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac aaccaaagca tttcctgcgc
    52021 ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa acggaaggga tttccagcaa
    52081 cgttaaggca tcgcgcacct gggcggcctc ctggcggctg cgcaccagca cactgatgtc
    52141 cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc cccgctgtcc
    52201 ggcttgtagc cagtcgcgaa tttgcgcagc acttacctgc gccatggtac tttgatagtc
    52261 gccaacgccg caactttcgc cttccatcag ccacattttc atcgcaggct gcgtttcacc
    52321 tttaaagaca aaacgtaacg cctgattttt cccggctgat ttcactggaa taaacggtat
    52381 ttcgcgaaac atgaacgcgt catcagtctg gctgaaaagc ttgttcacac tgttcaccat
    52441 tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg gcgtgaactt cactacgcgc
    52501 cttcatataa gtgaagatat ccgcaccccg gaatgcatat atggcctgct tcgggtcgcc
    52561 aatcagcaac aatgcggttt ccggctgatg gtgccagata cggcgaaaaa ttcggtactg
    52621 ctgggggtcg gtatcctgaa attcatcgat cattgccacc gggaatcgcg tacggatcgc
    52681 cgctgccaac acctcaccgc tttcgctacg cagcgcggaa tcgagccgac ttaacatgtc
    52741 atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct actgtttcgc ggatctcagc
    52801 caatgcgcgg gtgatcacca gatcgcggat cgacaatggt tctgcaagca gttgatcgat
    52861 cgcctcaaac agtggatgtc gcggggtttc ccccccggcc ttcgtgcgat cttctaagaa
    52921 acgctgggag aatttttcca gcgactccgg caactgataa ctgtttctct cttcttctgc
    52981 ccaggcgctg atcttgtcga tccatttagc ctgattgcta cggttaaact tgcgtcgatc
    53041 aataccagaa gattcgatca gcgcatccag ttcacccact gcgtcgcgcc actgctgttt
    53101 taccgtatca atacgcgcca caatttgcgc gtgacgggaa gccagcgttt catcatcggg
    53161 cggcggtgct ttgataaccg gcgcttcacc ttgcagataa cgattaatat cgcgcagcaa
    53221 cgcctgcggc cctttccagg tttcaaagac gaccagggct atttcacgcg gcagcgggta
    53281 gcagtggcga cgccagaaat cggcgcaggc ctggtaacgt agcagagact catcttcaat
    53341 cagctgctgc tcaaacagca tgccggattc aaaggcattc aggttgagca tgcgctggca
    53401 aaaaccgtga atagtaaaga ctgccgcttc atccatctgc cgttcggcta acaacaacca
    53461 cttcgcggct tgcgctttat cgtcgatctc ttccagcaga cgttcgtaca gtggattgtc
    53521 ggtggtttca cgcagacagg cgatgcgcaa ctcatggata ttgctacgga tacgaccacg
    53581 caattctgcc gtggcagcct cggtaaaggt gaccaccagc agttcttcaa cggtcagcgg
    53641 gcggggaaag gcggcggaac cgcctagtcc aagtaacagg cgcaaataga gcgccgcaat
    53701 cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg cgctcgccct gaaagggcaa
    53761 gcgcaaagga tctagtgtct cggcgacatc actcattctt ttcactcatc aggggcattg
    53821 tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcaggatgc acatattcgg
    53881 ctttcccgtt ctggctgccg gaaatctgcg acagaatagc catgccttgc ggctcgacca
    53941 ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag cagttttatc tgggccacga
    54001 ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt aacttcaatg
    54061 cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc tgctggattt
    54121 gcgcaaactc atctggcttc atcgctcgca attttgcctc tgcggttggg aaaaacgcct
    54181 tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc
    54241 cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaat tgttcttcgg
    54301 tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag ctataggctg
    54361 agcttgtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg gagtcggtgc
    54421 tgttaccggc tttttcaaag atgacggatt gttttttatc gaccactaca tctttgtttc
    54481 gacaccactc tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc agcgttgttg
    54541 cctgggcctc ggtcatgttg ccgataacca taaactctgg tcgagcccct gattttaagg
    54601 cgtcacgata agccagcacc tctttcaacg taatggaggg caaaatttta cgccgttcat
    54661 ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc tgctcaaacg
    54721 ctttaccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc tgctcaagct
    54781 gatcttccgt agcggtatag ctaaagtatc cctcgagcaa tgcctggaac agctgcggca
    54841 gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg gtggaaaaac
    54901 ttatgccacc aaccgacgcc tggttgctta actgatcaag cgccagccct gcgagataat
    54961 cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa
    55021 tcaggctgac atcagctttg ggttcgctgg aaaaataacg gcttggcgca tacaccacgc
    55081 gcagattcga ctcatcaaca atcagctctg gatggtcata tttcttctct gacttaatca
    55141 gcgagaaatc gtcaggaata taggggttaa gctccggtaa ggagagcgca atattggcag
    55201 ctttttgctg ccagtcagcg aaagtttgtt cgctgatttt atcgacctga tacggcgcat
    55261 cgacaaaata agccgtttta ttgtgcggct ctttcgggct gatataccag atacgcgcat
    55321 tctgcggcgt catcatcgcc agacgttctt ttactgcttt agcatcgtac cgatcggcaa
    55381 tattgactgc atccagcgta tgctcaacag gaacgcgaat catggtatct gccagccatt
    55441 cgacgtaatc catatcacgg gtgattgacg gataacggaa gtcgatatcc agcacattcg
    55501 ccagttcatc gaagtattgt ttatcgatcc ctttttcacg taacaaattg aggtagctaa
    55561 aaatagccgc cacaacctga tcgcgattcg ccagaccttt atcggttaaa gacgcagaga
    55621 tcgctaatac gccgctgttg ccgttgacga taggatcgga gttggcgcta atgccctcaa
    55681 ctaacccctg cttttgcagc cagtcagaca gtgtacctgg gctgcgatta ccaatcagat
    55741 aggtaatcaa ttcatccgtt ttactgcgga acttcgctga gttgttatcg atgcgaaact
    55801 caacgcgcag aactttacgc ggcagcgctg ggacgtaatg aatgataatg cccttttgcg
    55861 cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc ggcacgcgac
    55921 caaaagtgtc cgccgccatt tttgccaact ccggcagcgg tttattactg taaataaccg
    55981 ccttcatcaa attggcggaa tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg
    56041 gattaccagg tttgtcgctt aaagtttcga ggttaccacc agaaaacttt gaaccggggt
    56101 gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca cgtgtacgcg
    56161 ccatggttaa ttcagcgttc actgcattac gctcacgttc ggcgtatttc ttgtcgagca
    56221 agggttctgc aatagcatcg gccaggcggt ctaccgcacc gggcaaggcg tcgttctcaa
    56281 cttccagata gaaagccgtg cgatacggcg cagtgctggc attgtgacta ccgccgtgca
    56341 ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc atcagactca
    56401 tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc gacccaacgg
    56461 gcaccaccag cgccgatagc gatttaactg cctgcggatc agaaaccagc aagaccacca
    56521 taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt ttacggatgg
    56581 tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa agggcaacta
    56641 acaacaataa tgctttgaac caggtgctgc ggggcattca cggacctcat aatcaacttt
    56701 attttctggc cagattcaac aggttaacgc aaatcatctg ccagaattta atcttgtgct
    56761 gcacgagtca gcctatgttt atataaccat cagtccgtga ctggtgcgca tcataaagta
    56821 agcggataga ttgcgcaatt tttatacagc gctcatgact gattaaagcg aaacagcggt
    56881 aacaggaaac gttgcgactg ttcaacgatt gcctccattg tctctggtgt taattgccgc
    56941 cagagccttt gataccagat atcatcacct tcgccactca ccatcatgtt gccttcgtaa
    57001 gcctgaagga atttcgtacg ggctttttgc aacgtggaat cgtcatccag catggcatcg
    57061 ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa caccagcaat
    57121 ggcgcggaca tcccttcacg atacccctca atcagttgtg agaggtaatg caaagcctgt
    57181 tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc gtagaaaaag gcgactttca
    57241 ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat tccctgcgcc
    57301 acacttaata aagagggacg ccagcgcaac aagccatccg gctgcacctg cggcaaccag
    57361 ccagttatct gcacaccgtt gcaagcgaga tcaatttcca tactctgccc cggctggcga
    57421 caggcaatga ctctgtcggc aagctgctgc atctcctggc actgtgtttc ccagaaaatt
    57481 tcaccaaaag cgccatacgg taaatcccct gccgcccgga agcggcggaa caagcgttcg
    57541 gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata acggctaagt
    57601 ccttccagaa taaatggctc ggtgtcgggg atttcgctgt cttcagtacg gaagttcacc
    57661 tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc agaatcgttg tagcgtttcc
    57721 agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga atgtgcttta
    57781 ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc gcctggctgg
    57841 tagttttgcg gatcaaacgg catccgggta tggaggcaag taagatgcgc ttttaccctt
    57901 gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatgact ttgcccgatg
    57961 tagtcgatca gttcctgcac cagtaccgac gggaaacgtt cactgttatc ctgaatggaa
    58021 cgcccgatat agctgatata gagtttttgc tgcgcggaaa ttaacgcttc caggaacagg
    58081 tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg gtttctggct catcaggtca
    58141 aagcccaatg gcgcaagctg acgtggataa acgccgtcgt tcattcccag caggcaaacc
    58201 actttgaacg gaattgaacg cattggcatc agagtacaaa tgttaaccgg cccggcgaga
    58261 aaacgctggc tgatacgttc ttgatccagg cgctgtgcca gttcatcacg caatagtgac
    58321 agcggcaccg catcgccata ctgcgcacct aaaccttcgg cgataatcgc ctgccattgt
    58381 tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagag ggcgttgagc
    58441 atatcgcgac aaaccggcaa ccactcttcc agcggacgct cctgtgccag cccgcgacgc
    58501 cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc aattaagccg
    58561 ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc catcgcgtag
    58621 cccaacaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc ggggagttcc
    58681 agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc cggattcgtt gacccactga
    58741 cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac cggcacatcc
    58801 agtaatgcca gcacgtcttc cgacacaaag cggctgtctg gcaacgataa caggctgata
    58861 aacgcttcaa gtacaggatg cgactgccgc gcccgacggt cggaaatggc gtaaggcagg
    58921 taacgatccg caggtgcgct accaaacaca gcctgaataa acggactgta gctgtcgata
    58981 tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg ggtcttcttc cagcatcgcc
    59041 agcaggcgat cgtgtaaaac ttcaacttca cgctgcgggc tatggcaaac gtggaaggtg
    59101 atactgctat ccagtggatc aagcggacgt ttgttatcgc tacgggaaaa ctcttcgatg
    59161 ttcacaccag caacggcgcg gttttccagt tccagaatgt cagactgaat gttatgcagc
    59221 aggttatctg gcgtcacatc gacaaaggcg tccagctcct ggctgctctc caggtcagaa
    59281 aggagataaa tgtagtcgcg cccaagcttg ccccatgagg ccagcagcgg gttgccgaca
    59341 tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctgtcgcg aaatagcggt
    59401 aattcgtgat cttcaaaact gtgtcggcgc tggcgagtca gtagtttcgc cagataagca
    59461 ggatctttaa tgtcgcccca gtaataacgg caggggttgg taaacaggag atggatttca
    59521 atatgtttac ccagcgcctg gagcgcctgg agataaacag gcggtaacgc ggaaataccg
    59581 catataaaga cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc cagcgtttcg
    59641 ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gtccgagttc atgggtatat
    59701 tccaccagcg ccttccacaa cggcgcttgc caggcctgtg cctctcccag cccttcaacc
    59761 aaatgtcctg tttcccactg tgccagccag tccggacgat agaccagata ctggtcaaac
    59821 aggtccgccg cttttgagga aagctggaac agctttcgct tgtcgctatc gtcagtcaga
    59881 taatgccgca acagggtaaa gtcttcgcgc tccagcaatt gcggcagcag agtcatcagt
    59941 ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatctccgg taacacccgg
    60001 acgaacatat cccagataaa gctcgcaggc agcggaaaat caatgtttgc cgcaatacca
    60061 aacttttgcg acagggtcat ttgcagccac tgtgccatac cggtactttg caccagaatc
    60121 atctctggtt cgaaaggatc gtccagccgt tcgcgttcga caataaactc catcaacgct
    60181 tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc tgactactga
    60241 cgattcgggc aatgcaggcg ggtcatctcg cctgctctgc ccccgggtga aactagcgta
    60301 acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg ccagttggca
    60361 ggtggcgaaa tcgcgcgcaa ttgcgtttgc tgccaaccat gccgccagag ctgttggtac
    60421 tggtttctgc tggtaagact gttcattaat gttcgctgga aacccgataa cgcagtgaca
    60481 atcatcacca tcaacaccat cgccaacatt acctccggca ggctaaagcc ttgttgattc
    60541 ttcagggaag ctgacataac gccccctctt tcagcggaca aaaatcgctc cagccgcgtg
    60601 gcgaaaaaac aatttttcca tcaatgactt cgcctgttcg ccacaacgaa acgccttcat
    60661 aaccggcaat taataaggct tcattatctt ccagtaaacg caaacaaacc cgggcaccag
    60721 tggcagcgta cagtaagcac tgaactgcgg gctgcgtctg ccaggagtgc attttccccc
    60781 acgccaacgc cgactgaacg atggcctggc ggcgcagtga ctgactttcc atgctcacgc
    60841 gagaggcaaa actgcgatcc tgctggctca ttccttgtaa tagcaagcta cccaaaacca
    60901 gcagcatcag gaccagagcc agtgacgaaa cacccttttc gcggttcaca ggttgaatcc
    60961 tgtcacgcta tagctggcat ccaccacggt ttgcggttca gacttactgg cagcacgcat
    61021 attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct gaaaagtgtc
    61081 gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac aggatgtcgc
    61141 accgcgtagc gtttccaaca cttgctcctt cagacgaaat ccaatctggt cggactcttt
    61201 taccggttcg cgatcccaga taccgttact gttcgcatcc cactgcacaa tgatacagtc
    61261 accctgtccg acaatttcca gcccttcgcc ggtacagatg ccatgacaat aacccgccct
    61321 ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca gtgccagctt
    61381 acgggtgctc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgcag cccccaacaa
    61441 caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac cttgctcttt
    61501 tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca cggcgaaacc
    61561 accagccacc actcgcccgt tgagtttttg aagccaatat gcccggccca tgcggtattg
    61621 cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc gggccagcgt
    61681 ggcacaaaga ccaatggtga actgccatga caggtattcg ccccagcaac ggaactcaca
    61741 aggcaccata acgtcccctc cctgataaaa ctgatactgt ggtcgcggtt atgccagttg
    61801 gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg ccaaagccgt
    61861 tgcgactgct gccagtattg ccagccatag agtccacttg cgcttagcat gaccaaaatc
    61921 agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat gccggcagta
    61981 tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcgca tcttccggac
    62041 tatttacgcc gttgcaggac gttgcaaaat ttcgggaagg cgtctcgaag aatttaacgg
    62101 agggtaaaaa aaccgacgca cactggcgtc ggctctggca ggatgtttcg taattagata
    62161 gccaccggcg ctttaatgcc cggatgtgga tcgtagcctt caatctcaaa gtcttcgaaa
    62221 cggtagtcga agatggattc gggtttacgt ttgataatca acttcggcag cggacgcggt
    62281 tcacggctta attgcagatg agtttgatcc atatgattac tgtacaggtg cgtatcgcca
    62341 ccggtccaga caaaatcacc cacttccaga tcgcactgct gcgccatcat atgcaccagt
    62401 aacgcgtagc tggcaatgtt gaacggcagg ccgaggaaga cgtcacagga gcgctgataa
    62461 agctggcaag agagtttgcc gtctgccaca tagaactgga agaatgcatg gcacggtgcc
    62521 agcgccattt tatccagttc gcctacgttc cacgctgaaa caataatgcg gcgggaatcc
    62581 gggtcgtttt tcagctggtt cagtaccgta gtgatctggt caatatgacg accatctggc
    62641 gttggccagg cgcgccactg tttaccatac actggtccga ggtcgccgtt ttcatcggcc
    62701 cattcgtccc agatggtgac attgttttcg tgtagataag caatgttagt gtcgccctgc
    62761 agaaaccaca gcagttcatg gatgatggaa cgcaggtggc aacgtttagt tgtcaccagc
    62821 gggaatccat cctgcaggtt aaaacgcatc tgatgaccaa aaatggaaag cgttccggtt
    62881 ccggtacggt cgtttttctg tgtgccttcg tcgagcactt tttgcatcag ttctaaatac
    62941 tgtttcatgg ttcctcagga aacgtgttgc tgtgggctgc gacgatatgc ccagaccatc
    63001 atgatcacac ccgcgacaat catcgggatg gaaagaattt gccccatgct gatgtactgc
    63061 acccaggcac cggtaaactg cgcgtcgggc tggcggaaaa actcaacaat gatgcgaaac
    63121 gcgccgtagc caatcaggaa caagccagac acggctccca tcgggcgcgg tttacgaata
    63181 tacaggttga ggataataaa cagcaccaca ccttccagca gcagctcgta aagctgtgat
    63241 gggtggcgcg gcagcacacc gtaagtgtcg aaaatggatt gccactgcgg gttggtttgc
    63301 agcagcaaaa tatcttctgt acgggagcct gggaacagca tggcaaacgg gaagttcggg
    63361 tcaacgcggc cccacaattc accgttaata aagttgccca aacgcccggc accaagacca
    63421 aacggaatga gtggtgcgat aaaatcagaa acctggaaga aggaacgttt agtacggcgg
    63481 gcaaagataa tcatcaccac gataacgcca atcaggccgc cgtggaaaga catgccgccg
    63541 tcccagacac ggaacagata cagcggatcg gccataaact gcgggaaatt gtagaacaga
    63601 acataaccaa tacgtccccc gaggaagacg ccgaggaagc ccgcatagag taagttttca
    63661 acttcatttt tggtccagcc gctgcccgga cgattcgccc gtcgtgttgc cagccacatt
    63721 gcaaaaatga aacccaccag atacatcagg ccgtaccagt gaagcgccac gggtcctatt
    63781 gagaaaatga ccggatcaaa ctccggaaaa tgcagatagc tactggtcat ctgtcaccac
    63841 aagttcttgt tatttcgctg aaagagaaca gcgattgaaa tgcgcgccgc aggtttcagg
    63901 cgctccaaag gtgcgaataa tagcacaagg ggacctggct ggttgccgga taccgttaaa
    63961 agatatgtat atgatccgcg ctataaccct ccgcgaatca gcccgcccat gccgcgacgc
    64021 tccataaagg ctgcaacctg atggcgaact tcggtcgcca gttgcgcttc cagactacgc
    64081 tgcgcaagat tttctgcttc ggcataatca atgcgccgca gcaggtattt tacccgcgct
    64141 acagaacgtc cgttcataga cagatggcga tacccaagcc caatgaggat tgccacgcac
    64201 atgggatcgc ccgccatttc accgcacaaa cggagatcga ttccatgtat ttccgcttcc
    64261 cgggcgatca tcgccagagc tcgtaacatt gcaggatgaa gactgtcata aatgttcgcc
    64321 acccgggtat tgttgcgatc aacggccaga atgtattgag tcagatcgtt ggtgccaaca
    64381 gagatgaaat cgacccgctt tgccagatgc ggcagcataa ataccattga cggcacttcc
    64441 agcatgatgc cgatacgtgg tttgggaatt tcgtaaccga tcatctcctc gacttcacgt
    64501 ccggcacgtt caatcaggcg gcgtgcttcg tcaacttcat cgaggcttgt gaccatcggc
    64561 aacagaatat tcaggttgcc cgtagcggca ttagcacgca gcatcgcccg cacctggatc
    64621 aagaagatct ccggctgatc gagcgtaatg cgaatcccac gccaacccag gcatggattc
    64681 tcttcgctaa tcggcatgta aggcagctgc ttatctgctc cgacatccag cgtacgcaag
    64741 gtgacgggtt tatcattgaa catttgcagc atcccctgat actgcgccac ctgttcttct
    64801 tccgacggaa aaccactttg cagcatgaat gggatttcag tgcgataaag accgatgcca
    64861 tcaatacggc tgcccagttt ttcttcatgt tccgggctta aaccagcatt gagcatgact
    64921 tttatacgct caccgctttt taactgcgcg ggtaaattga cgtcatcttc cgccagacgg
    64981 ctaagctcaa tctcttcact aattagccgc tgatattctt gcagcagtac cggctccgga
    65041 tcgaccagca attcaccgcg atagccatcg acgatcagcg tccgacgatg cagcaccgaa
    65101 ggctgaatat ccgcgcccat cacggtaggg atccccagcg cacgtaccat gatcgcagca
    65161 tgggagttgg ctgcgccatc tcgcacgaca acaccgacta agcgatcctg gggcagctca
    65221 gcaagagttg tcgctgacag ttcatctgcc accagaatga aacgttccgg ccaggcgttc
    65281 ggcccttgat tagcgtcatc aagatgaaac agcaatcgct gacccagcgc acgtaaatcg
    65341 ccagcccgct ctttgagata gttatcgctt agcgcggcaa actgttcggc aaatttttca
    65401 atgaccgttt ttaccgccca ttctgccacc gagcctttat caacctcggc aaacaattcg
    65461 cgacgcagcc gggtgtccga aagcaggtgc gagtaaagat cgaaaatagc cgccgtttct
    65521 ttttgtgcac cggcggcaaa gcgtttgctg tagcggcgaa actcgtttgc cgcttcttcc
    65581 agcgccccgg tcagtcgttc gcgttccaga gccggatcca gcgttgatgc ctgatacacc
    65641 tgttccatta aaggtaacgt ggcatcctgc cagccttcgg caatcgctac accaggtgct
    65701 gccggtaatg cgcggattcg cgtctggcga tattgcccaa acaaggcagt caactgcgac
    65761 tgagaaagaa tcgctgccat ctgggtggca agcgtcacca ggaaggattc ttcactttcg
    65821 tcatactggc gcaactctcg ttgctgtacc accagtacac caagcaactg gcgacgttga
    65881 ataattggta cgcctaaaaa cgcgcggaaa cgttcttctt ttacggaggg gatgtatttg
    65941 aagctggggt gcttttgcgc atctgcaagg tttatcggtt ccgccagcct gccaaccagg
    66001 ccgacgatcc cttcatcaaa ggcgagcgtt acagtgcgac cgcgtggttt tttcagcccc
    66061 cgggtcgcca tcaggtagta acaacgtcga tcatgatcgg ccaggtagac cgaacagacc
    66121 tcggtatcca tcgcaagaca gatgtcggta accagaatat ttaacgcctc attcaggcgt
    66181 ggtgcgctgg ctaccttttc gactatttcg cgcaggcgag tgagcataat tggcgtgact
    66241 taacctcttt tacgtcgata agcagatgcg ttttgtggtt ttggcgtatt ttcctgcagt
    66301 gacatcacca cactcgcgaa ctctttcatt accctacggt agacatcacg tttaaatgac
    66361 accacctgtc tgaccggata ccagtaactt acccatcgcc agccgtcaaa ctctggtgta
    66421 ctgctggttt gcatattgat ttctgcatcg ccgctcacca gctgcaagag aaaccatttt
    66481 tgtttttggc cgatacaaac cggcttcgtg tcccaacgca ccaaacgttt cggtaatttg
    66541 tagcgcaacc agttacgcgt tgaagcaagg attcgaacgt ctttgcggct taatcctact
    66601 tcttcaaaca attcacggta catcgcctgc tctgcggatt ctccggggtt gattccgcct
    66661 tgcggaaatt gccaggagtg ctgaccaaat cgccgggccc acattacctg cccctggcga
    66721 ttacaaatca cgatacctac gtttgggcgg tagccatcgt catcaatcac cggactacct
    66781 caaaataaag ctttatatac gaatgattgt ttcatactcc aggaagacgg taaaccactc
    66841 tctgcagggc attacacact aacaacaatt gaataactca cagttatgtg cagagttata
    66901 aacagaggaa ggggtggata gccgttttta ttcacctttt ctgtggatag agttgtgaag
    66961 aactacggaa ttactacggg aaaacccgga gcattctgaa taagcccgat atagacattt
    67021 taaaatattc attattagtc aatgcattac atgtaattta agtaatgaaa aagagtaatt
    67081 cgtgacccag gtcacacctc tcatttacgg gttggcgaaa gatcaaccaa tgccgtattt
    67141 atccacagaa tgtgccacta agttaagcac tgaaccacta aaaactggag tttcgtcgca
    67201 cgtcaaggct gtaaatggaa acagtagtgg aggtttttca cagttatccc agctttctgt
    67261 ggataacatg gtgtaagatc ctgtttattt tcagtgacca gatttggaaa acccgttgca
    67321 gtgttgcgca actcgattac cggcaaccta aaaagcaata taaatcagat aattgaacaa
    67381 cgcatgtgga aaaagttaca ctgcgaatat tcggcacata attgctgttt gttttttaac
    67441 caaggtatca tgacatgtcc caacctcgcc cactgctctc tcctcccgaa actgaagaac
    67501 agttgttagc gcaagcacag caactttctg gttatacatt gggagaactg gcggcacttg
    67561 ccgggctggt tacgccagag aatttaaaac gcgataaggg ctggattggc gtgttactgg
    67621 agatctggct aggtgccagc gcagggagta aacctgaaca agattttgct gctctgggcg
    67681 tggaacttaa aactatccct gtggatagtc ttggtcgtcc gctggaaaca acattcgttt
    67741 gtgttgcccc gttaacgggc aatagcggag tgacctggga aaccagccac gtgcgccaca
    67801 aactcaaacg cgtactgtgg ataccggttg aaggcgagcg cagcatcccg ctggcgcagc
    67861 gtcgcgttgg atcaccgttg ctgtggagcc cgaatgaaga ggaagaccgg cagctacgcg
    67921 aagactggga agaattaatg gatatgattg ttctcggtca ggttgagcgg attaccgctc
    67981 gtcacgggga gtatttacag atacgaccga aagcagcgaa tgcgaaagcg cttaccgaag
    68041 ccattggtgc acggggcgaa cggattctga cgctgccgcg cggcttttat ttgaagaaga
    68101 atttcaccag tgcactactg gcccgtcatt ttctgatcca gtagccatcg ctttgacctg
    68161 ccgctttccg ggcatataat taccgcttca tttttttggc agggcttttt agatgttatt
    68221 tgcatggata accgatccta acgcctggct tgcgctcggt acgctgacgc tgctggagat
    68281 cgttcttggg atcgacaata ttattttcct ttctctggtg gtggcaaagc tccccacagc
    68341 acaacgtgct catgcgcgcc gtctggggtt ggcgggagcc atggttatgc gtctggcgct
    68401 gctggcatca atcgcctggg ttacgcgcct gacgaatccg ctttttacaa tattcagtca
    68461 ggaaatttct gcccgtgact tgattctgct tctgggtggc ttgttcctta tctggaaagc
    68521 cagcaaggaa atccacgaat ccattgaagg tgaagaagaa gggctgaaaa cacgcgtttc
    68581 atcattcctc ggcgctatcg tgcagattat gctgctggat attatcttca gcctcgactc
    68641 ggtgattacc gctgtgggtc tgtcagatca cctgtttatt atgatggccg ccgtggtgat
    68701 tgccgtaggc gtgatgatgt tcgccgcgcg ctcgattggt gattttgtcg agcgccatcc
    68761 ttcggtaaaa atgctggcgc tctctttcct gattctggtg ggctttaccc tgattctgga
    68821 aagtttcgac atccacgtac cgaaaggtta catctacttc gcgatgttct tctctattgc
    68881 ggttgaaagc ctcaacctga ttcgcaacaa aaagaacccg ctctgatact ccgtacgctc
    68941 tcctgcggga gggcgtactc acttctgtta ccctcacagt ttaagatttt cctgctttca
    69001 agatatataa cgtcagttta taaacagact attatcatag gtgggccaaa tgaggataac
    69061 cggatgaaaa aatgggcagt aataatttct gcagtcggac tggcgtttgc tgtttccggg
    69121 tgttccagtg attacgtcat ggcgaccaaa gatggccgta tgattttgac cgatggaaaa
    69181 cctgaaattg atgatgatac cgggctggtg agttatcacg atcagcaagg taacgcgatg
    69241 caaattaacc gtgatgatgt ttcgcaaata attgaacgtt aacaaataag gtcagcatcc
    69301 ggctggcctt aagatttttc tctttccctt ttcccttccc tctgccattt ttatattcct
    69361 tatgtcgtga ttataaaaag gaaacggcta tgcaatatca ccgtataccc cacagttcgc
    69421 tggaagtcag cacgctgggg cttggcacga tgacgtttgg tgaacagaac agcgaagccg
    69481 acgcccacgc acaactcgac tatgccgtcg ctcagggcat taaccttatc gacgttgccg
    69541 aaatgtaccc agtacctccg cgccccgaaa cgcaagggtt aaccgaaacc tacgtcggca
    69601 actggctggc gaaacatggc agccgcgaaa agttaattat cgcctccaaa gtgagcggac
    69661 cgtcgcgcaa taatgacaag ggcatccgcc cggatcaggc gctggatcgg aagaatatcc
    69721 gcgaagcgct gcatgacagc ctcaagcgtc tgcagactga ttacctcgat ctttatcagg
    69781 tgcactggcc gcagcgccca accaactgct tcggcaaact cggttatagc tggacagatt
    69841 ctgcgcctgc ggtttcgctg ctggatacgc tggacgcact ggcagagtac caacgagcgg
    69901 gaaaaattcg ttatatcggc gtgtcgaacg aaactgcatt tggcgtaatg cgctacctgc
    69961 atctggcgga caaacacgat ctgccgcgta ttgtcaccat tcagaaccct tacagtctgt
    70021 taaaccgcag ttttgaagta ggtctggcag aagtcagcca gtatgaaggg gtcgaactgc
    70081 tggcctattc gtgcctgggt ttcggcacgc tgaccgggaa atatctcaat ggtgcaaaac
    70141 ccgctggcgc acgtaatacg ctctttagtc ggttcacccg ctatagcggt gagcaaacgc
    70201 aaaaagccgt cgcggcgtat gttgatatcg ccagacgtca tggcctggcc cctgctcaga
    70261 tggcgctcgc gtttgtacgc cgtcaaccgt ttgttgccag cactctgctg ggcgcaacca
    70321 cgatggatca gctgaaaact aacatcgaaa gtttgcatct ggagttaagc gaagacgtat
    70381 tagctgaaat tgaagcggtg catcaggttt atacttatcc ggcaccataa gattaacgcc
    70441 ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg ccgttattgc
    70501 cagcgcaaat agcgcaccga agccaatgcc aatgggcacg accgggatgc ctaccattac
    70561 cgccagcgag taaatgccca gcatcaacaa catggcgctg ttttcgccaa ggttttgtac
    70621 tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca acgcattgag
    70681 cggaacgaca aaaaagcccc ccaacacgcc aatcagcatc aacaaggcat aggctggcag
    70741 ctgctcgtgt tgcagggaga aaatcagtac caccacgcca atcaaaattc cggctggcat
    70801 acagcgtgac acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc caaccacgat
    70861 accaatcgct accatcgcgt tgagataggt cggcgtagcg ttatcggtaa tgcccagcgc
    70921 caccggtacc cacagcacca acaggaaacg cagcgtgaca cccgctcccc agaataaact
    70981 ggtgcccacc agcgaaaaac gcgtttcacc attgcgccat agcgaggtgc aggcattcag
    71041 gaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg ccgccagttt
    71101 gggaatgtag atattggcaa cgaccgcacc accgtaggcc agtgcgcatg cggccagggc
    71161 gaccaggaca tgccagtcag ccagcacacc accggctacg gaaccgagca aaatcgccgc
    71221 tatggtagaa gcttccatta aaccgttagc ttttactaac ttactacccg tggttaattc
    71281 gccgagaata ccgtatttcg ccggtgaata ggctgcagca ccaacaccca ccagcgtata
    71341 gccgagaaac ggattgatac caaagcagat actggctgcg cccagcagct tcaggccgtt
    71401 ggcaaacatc atcacccggc ctttggcgaa gctatccgcc acctgcccga caaacggcgc
    71461 aaaaagaatg taagcaccta caaacaccat ttgcaggatg ggctggctcc actccggata
    71521 gaactgcgct ttcagtaacg ccagagtggc aaacagtagg gcattatcgc caaacgcaga
    71581 gagaaactgc gccacgataa ccgctttcat ccccttcgac cacaacgaag tgttagtgtg
    71641 cactgactca ctcatcgtgt tgttccactt cgtctaccca gcttttcaac gtgacaaagt
    71701 caggtttgcc gctgccaagt aatggcatct gtttcaaata gcgaatatca cgcggtacag
    71761 caagctccgg cacgccgtgc tcacgggcat actgttgcaa cttatcgcgc gtcagttcgt
    71821 tatctgtggt gaaaagcacc agtgcctcgc ctttgctggc atcgctctta atcgcagtgg
    71881 catggacttt atctggcgaa acaccaagtg ccagttgttc caccatttcc agcgacacca
    71941 tttcgcctgc aattttggca aagcgttttg cgcggccctg aatctgcaca aagccctgct
    72001 cgtcaaaatg cacaatatcg ccagtgtcat accagccgcg ctccatttcg ccgcgaacat
    72061 tctcggcggt gggcacttcc agtacacctg gcttctccac ccgcagatag ccgttcatta
    72121 tgttcggccc tttcagttgc aggcgtccgc cctcttcgat accagggacc gacaacaggc
    72181 gcgcatccat tcctggtaga atacgcccta ccgtaccggg tttcgccgcc atcggtacgt
    72241 tgatagaaac gacaggcgcg cattcggtca caccgtagcc ttcaaggatg cgcaggccaa
    72301 atttatcctg ccaaagctgt ttggtacttt cttgtagttt ttctgcgcct gccaccacat
    72361 agcgtagacg atagaagtca tacgggttgg cgaaacgcgc gtagtgaccg aggaaagtcg
    72421 aagtgccgaa caacacggtg caactgcggt catacaccaa ctccggcaca atacggtaat
    72481 gcagcgggct tggataaaga aacacttctg cacctgtgag cagtggcgta aacaggccta
    72541 ccgtcagccc aaaggagtga aacagcggta acgccgacat aaagcgatcg ttggtggtga
    72601 agtcggcaat cgttttaatc tgctcgacat tcgccagaat gcttttatgg ctatggacga
    72661 cgccttttgg atggccttca gaaccggagg taaaaaggat cagcgcctct tcttccggct
    72721 gctgtttaac ctgtgccaga cgcggcatca gcaaatgagc gaagatccat actttgtcgg
    72781 cagtggtgac atctgctttt aaatcttcca gatagaccca gcgcacctga gtaagttgct
    72841 ccggcagatg ccagagtttg cctttatcga gaaactggcg ggaagtgaag atggttttga
    72901 tttcagccgc cgtaatagca ctggtcagcc cttttacccc agcagtgtag ttcatcattg
    72961 cgggaatgcg gcgacgggcg atggccccaa aaatcactgc cgcactgatg cctgcattgg
    73021 gcagcattaa gccgatgcgt tcgccttcaa cactgtattt ttcaaggatg cgtccaacaa
    73081 acagcgtttt cgtaagcaat ttgcgatagg agtctggggt aaaattgacg tcttcgacac
    73141 atttcttccc tgctccgaag cggtacattg cactcagtaa agattcgtac agcgtttcac
    73201 gcgggcgcac cgccattcgc gcttccatca ttatttgatg cagcatttcg ccagcgattt
    73261 tgcgacggtc acgggcacgc ggcgcatccg gcatctccac ctgcgttggt ggcaaaatat
    73321 gcagagtaat ttgcgggaac aagcgacgtt taaccagacc tttcaggcgg ctgaagtgcg
    73381 taagttccgc cccttcaata cgcacaggaa taaccgttgc accagacttc gccgcgacaa
    73441 aacccgcgcc atcgtagatt ttcatcagcg agcctgtggt ggtgatgcgt ccttcaggga
    73501 aaatcaccac tggtcggccc tgttccacca gacgtaccag atgtttaatc gccataggtt
    73561 gcgtcgggtc gagaggaaca aagtcgataa atgatttcag ccaacgcata taccactgtt
    73621 ggcttattga ggtgtaaacg gcaaacactg gacgcacagg taaaaacagt ccaagcaaaa
    73681 tgccatcaat aaaagagacg tgattaggcg taattagaac gcgctcgccc ttcagtgcct
    73741 gggggtcacc cgtaacgcga acgcgataca aaacacggca caaatttcga aaaaagctaa
    73801 aaagcattcc aactcccttt gctctgattc agtaaaagcg aatggaggga gattacacga
    73861 gataaagaac gcgagcgaca gtaaattagg tgcgaaaaaa aacctgcgca tccgcgcagg
    73921 ttggtgcaag agacagggta cgaagagcgt accgaataat ctcaccaatc aatacctctg
    73981 ggatcttgat tgtggtctgc acgacgactc ttcgccagcg agaaaacgca aaggaatgaa
    74041 gggaaatgca acgaggtgtg taaattgtcg gttactgtta cagattgatg accggcaaaa
    74101 aaaaacctgc gcatctgcgc aggctggtgt aattcatgtg ctcaacccga agttgacttc
    74161 acctatcaat acctctggga tcaccacttt agcaacctga agccaaacgc caccagcggt
    74221 caatcgcaac agcgtttcgc aaagtgtaac caaaggtttg caatctcttt ttttgtctta
    74281 cttatttgcg tttggctcac attcccacga tgaaaacacg ccgccccttg aaccaacggg
    74341 cgttttccgt aacactgaaa caatgtaagc gtttacccac taaggtattt tcatggcgac
    74401 cataaaggat gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa
    74461 taattcaccc aaagccagcg aagcttcccg gctggctgtg catagtgcaa tggagtctct
    74521 tagctatcac ccgaacgcca acgcccgtgc gctggcgcag cagaccactg aaacggtcgg
    74581 tctggtcgtt ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca
    74641 ggtggcttat cacaccggta atttcttatt gattggcaat ggttaccaca acgaacaaaa
    74701 agagcgtcag gccattgagc aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc
    74761 caaaatgatc ccggatgccg atttagcctc attattgaaa caaatgcccg gtatggtgct
    74821 gatcaaccgt atcctgcctg gctttgaaaa ccgttgtatt gctctggacg atcgttacgg
    74881 tgcctggctg gctacgcgtc atttaattca gcaaggtcat acccgcattg gttatctgtg
    74941 ctctaaccac tctatttctg acgccgaaga tcgtctgcaa gggtattacg atgcccttgc
    75001 tgaaagtggt attgcggcca atgaccggct ggtgacattt ggcgaaccag acgaaagcgg
    75061 cggcgaacag gcaatgaccg agcttttggg acgaggaaga aatttcactg cggtagcctg
    75121 ttataacgat tcaatggcgg cgggtgcgat gggcgttctc aatgataatg gtattgatgt
    75181 accgggtgag atttcgttaa ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc
    75241 gcgcctgacc accgtgcgtt acccaatcgt gacgatggcg acccaggctg ccgaactggc
    75301 tttggcgctg gcggataatc gccctctccc ggaaatcact aatgtcttta gtccgacgct
    75361 ggtacgtcgt cattcagtgt caactccgtc gctggaggca agtcatcatg caaccagcga
    75421 ctaaccgcag ttaaagcaat tccagcgcca gtaattcttc gatggtctgg cgacggcgaa
    75481 tcaaccgcgc ctgaccatta tcaaacagaa cttctggtaa cagcggacgg ctattgtagt
    75541 tggatgacat tgatgcgcca tatgcccctg tatcatgcag taccagataa tcacctgcct
    75601 tcacttccgg caaggcgcgg ggttcaacat ttcccccttc ctgctgggta aagacatcgc
    75661 ccgattcaca taacggtccg gcgacgacgg tttccaccgt tggtgcgtgt tccagagaac
    75721 gaccatcagc tgccagggca ctgatatggt ggtaactacc gtacattgcc gggcgcatca
    75781 gatcgttaaa cccggcatca accagcacaa agtggcggct acccatttgt ttgacgctcc
    75841 gcacctgagt aattaacacg ccagactgcg ctaccaggaa gcgacccggt tcaatttcca
    75901 gtttcacagg gtggcccaaa tggcgggcga tttgctcacg cgcggcattc cacagaccat
    75961 aataatgttc ggtatcaacc gcctcttcac cctgttgata aggaatagaa agcccaccgc
    76021 ccgcagaaat agcctgtaaa tcctgaccga attcgatgac ctgacgcacc atagcaccac
    76081 acacctgttc cagatgggca taatcaacgc cagaaccaat gtgcatgtga atgccgacca
    76141 gctgcagatg atgacgttgt atcacgtcca gtgcggcggg cagatcggtg taccagatac
    76201 cgtgcttgct gttttcgcca ccggtattgg ttttttggct atgtccgtga ccaaaccccg
    76261 gattaacgcg cagccatacc cgatgccctg gcgaaacctg gcccagttgg tcgaacatat
    76321 caacagaacc cgcattcacc ggaatttgca attcactgac gcgttcaagc gtcgcctgat
    76381 cgataacatc tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg taacccgccg
    76441 ccaacgcacg ctctatttcg cctaacgaga cggaatccac tttcacgccc tgctcacgca
    76501 ttaagcgcaa aatatgaata ttggaacagg ctttctgtgc aaagcgcacc acatcaaact
    76561 gtttcagcgc tgcaatctgc cgacgaataa tttgcgcatc gtagacccac accgggcagc
    76621 caaattcagc gggcaaacgc agcagatttt cggcggtgag atcggtatcg gtgctgaaca
    76681 gtgaatgtgg cataacaaac tccagatgag tgctttttta tgattacgcc acatcataaa
    76741 aagaataaaa aatatcgatt tatgtcgagt ctatgcaaaa atgatatgga ttaccggatt
    76801 gcgagagagc gctaatggcc gccgttaact tacgtcatat tgaaattttt catgcggtaa
    76861 tgaccgccgg aagcctgact gaggcagcac acctgctaca cacctcacag ccaaccgtca
    76921 gccgcgaact ggcgcgcttt gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg
    76981 gacgattaca tcctaccgtg caaggactgc gtctgtttga agaagtgcaa cgatcctggt
    77041 acggactgga tcgcattgtc agcgccgcag aaagtctgcg cgagtttcgc cagggagaac
    77101 tgtctattgc ctgcctgccg gtcttttcgc aatctttttt accgcagctc ctgcaaccct
    77161 ttctggtacg ttatcccgat gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg
    77221 aagagtggct ctcggcccag cgtcatgatt taggactcac tgagacactc catacgcctg
    77281 cgggaacaga acgtaccgaa ttactctctt tagatgaatt gtgtgtgtta cctccgggtc
    77341 atccgctggc ggtaaaaaag gtattaacgc cggatgattt tcagggtgag aactacatca
    77401 gcctttcccg tactgacagc tatcgccagt tgctggatca gctatttact gaacatcagg
    77461 ttaaacgacg catgatcgta gaaacccaca gcgccgcgtc agtctgcgca atggtacggg
    77521 cgggggtagg tgtttcggtg gttaacccgc tcaccgcact ggattatgcg gcaagcggtt
    77581 tagtggtgcg gcggttcagt attgcggttc cgttcaccgt cagcctgatc cgccccctgc
    77641 accgcccgtc atcagcgctg gtgcaggcgt ttagtgagca tttacaagcg gggttaccga
    77701 aactggtcac ttctcttgac gctattttgt cgtcagctac gacagcataa aagcgacagc
    77761 atcctcggca tggatcgccg cagtatcaaa cacaggcaaa acactgcgct cttctggcac
    77821 cagtaaacca atttctgtgc agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg
    77881 agcaatcact tgcgcataat aagcgcgtga cgcttcggta aattgcccca gacacagttc
    77941 ttcaaaaata atctgattaa ttttcgcccg ttcatccgct tcaggaataa gacaattgat
    78001 ggaaaattgt tccgtcagcc gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc
    78061 cagcagcgcc acacgagtca ttccggcccc ggtaattgca cgtccggtgg catccgcaat
    78121 gtgtaagaaa ggcagagtgc aacgtgactc aatggcatcc gccactttat gcatcgtatt
    78181 ggtacataac acaatacctt ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag
    78241 aatgtccccg gttttatccc attccccgcg acgctggcac tcttctattt catgaaaatc
    78301 gacgctatgt agcagcactt gcgcagagtg aagcccacca agccgctgtt taatgccttc
    78361 atttatcaga cgatagtaag gaatggtgga ttcccagctc attcctccca gcaaaccaat
    78421 tgttttcatt cctctctcct gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac
    78481 aaacgcctca acggccaggt gcccgatctc tattaacgaa aaaagggccg gatgtacagc
    78541 acatccggcc cgtgaaatca gacgccgata tttctcaact tctcgcctgc catcagtttg
    78601 cgttcgatat gttccagcgt gacatttttg gtttccggaa tgagccagaa agtgatgccc
    78661 acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac cggcagcgcc aatgctatca
    78721 agcagtgtca ggaaggtcgc gccgataatc atattcgaca cccagtttgt cgtcgtcgaa
    78781 caggtaatac cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc
    78841 actggcgcgg cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc
    78901 caggacaagc cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc
    78961 agagtgccta acgccatcac gctgaaacca attttcagag ccggtttacg acctgcttta
    79021 tctaccgtaa agaccgcaat gaaggtcgcg aacataaagg tcagtcccac gaccagagtc
    79081 gcaatcatct gttgttctgt ggtcgtaaag cccgccattt tgaagatacg cggcgcatag
    79141 tacatgatga tgttcatacc ggtaaactgc tgcatcgcct gcaacaacat accgagaaac
    79201 acagcacgac ggacgttacg gttgatctta aacagtgccc aaccgccttg ttttaacttc
    79261 aggctttcac gaatttcgtt gagttcttct cgcgcttttt ccgacgtatc gcgcagcata
    79321 cgcaacactt cttccgcctc aatatgacgc cccttttccg ccagccagcg cgggctattt
    79381 ggcaggaaga ccaccagaat aatcagcagg actgctggta aagcaagaac ccccaacatt
    79441 gcgcgccagt taccgctata actgaacgct gtatcggata aaaacgccag cacgatgccg
    79501 agtgtgacca tcaactggta catactgatc atcttaccgc gaacgttttc acttgccatt
    79561 tcagaaagat acagaggagc ggtgtaagac gcgatcccga cagcaatgcc cagcaccaca
    79621 cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg accctataga accgagtaca
    79681 aacaggatgg cccccgccat caggctgtat ttacgcccca ggcggaacga cagccaacca
    79741 ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca ttcctgcaaa
    79801 cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat tacgccgata
    79861 tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat attcatacgc
    79921 cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt aaccattttt
    79981 tcctgccagc agagagtaag acatagtgaa aaaatacgtg aacaattcac acaggtgtca
    80041 ggtcggaaac agcataaata tggattaaat tgctgcg
//
LOCUS       DAZFTN010000009        78939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFTN010000009 DAZFTN010000000
VERSION     DAZFTN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(90..1040)
                     /gene="dusC"
                     /locus_tag="ACHKV2_000742"
     CDS             complement(90..1040)
                     /gene="dusC"
                     /locus_tag="ACHKV2_000742"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO6938677.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(1200..2393)
                     /locus_tag="ACHKV2_000743"
     CDS             complement(1200..2393)
                     /locus_tag="ACHKV2_000743"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO6938678.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2408..3052)
                     /gene="maiA"
                     /locus_tag="ACHKV2_000744"
     CDS             complement(2408..3052)
                     /gene="maiA"
                     /locus_tag="ACHKV2_000744"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO6938679.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3061..3762)
                     /locus_tag="ACHKV2_000745"
     CDS             complement(3061..3762)
                     /locus_tag="ACHKV2_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6938680.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3777..4805)
                     /gene="gtdA"
                     /locus_tag="ACHKV2_000746"
     CDS             complement(3777..4805)
                     /gene="gtdA"
                     /locus_tag="ACHKV2_000746"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO6938681.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4817..6175)
                     /locus_tag="ACHKV2_000747"
     CDS             complement(4817..6175)
                     /locus_tag="ACHKV2_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6938682.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6302..7210
                     /locus_tag="ACHKV2_000748"
     CDS             6302..7210
                     /locus_tag="ACHKV2_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6938683.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7341..7739
                     /gene="yohJ"
                     /locus_tag="ACHKV2_000749"
     CDS             7341..7739
                     /gene="yohJ"
                     /locus_tag="ACHKV2_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6938684.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7736..8431
                     /gene="yohK"
                     /locus_tag="ACHKV2_000750"
     CDS             7736..8431
                     /gene="yohK"
                     /locus_tag="ACHKV2_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO6938685.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            8561..9445
                     /gene="cdd"
                     /locus_tag="ACHKV2_000751"
     CDS             8561..9445
                     /gene="cdd"
                     /locus_tag="ACHKV2_000751"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO6938686.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9595..10314
                     /gene="sanA"
                     /locus_tag="ACHKV2_000752"
     CDS             9595..10314
                     /gene="sanA"
                     /locus_tag="ACHKV2_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO6938687.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10317..10556
                     /gene="yeiS"
                     /locus_tag="ACHKV2_000753"
     CDS             10317..10556
                     /gene="yeiS"
                     /locus_tag="ACHKV2_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO6938688.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10750..11988
                     /gene="preT"
                     /locus_tag="ACHKV2_000754"
     CDS             10750..11988
                     /gene="preT"
                     /locus_tag="ACHKV2_000754"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO6938689.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            11982..13217
                     /gene="preA"
                     /locus_tag="ACHKV2_000755"
     CDS             11982..13217
                     /gene="preA"
                     /locus_tag="ACHKV2_000755"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO6938690.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13460..14470)
                     /gene="mglC"
                     /locus_tag="ACHKV2_000756"
     CDS             complement(13460..14470)
                     /gene="mglC"
                     /locus_tag="ACHKV2_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO6938691.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14486..16006)
                     /gene="mglA"
                     /locus_tag="ACHKV2_000757"
     CDS             complement(14486..16006)
                     /gene="mglA"
                     /locus_tag="ACHKV2_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO6938692.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16067..17065)
                     /gene="mglB"
                     /locus_tag="ACHKV2_000758"
     CDS             complement(16067..17065)
                     /gene="mglB"
                     /locus_tag="ACHKV2_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO6938693.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17344..18384)
                     /gene="galS"
                     /locus_tag="ACHKV2_000759"
     CDS             complement(17344..18384)
                     /gene="galS"
                     /locus_tag="ACHKV2_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO6938694.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18526..19683)
                     /gene="yeiB"
                     /locus_tag="ACHKV2_000760"
     CDS             complement(18526..19683)
                     /gene="yeiB"
                     /locus_tag="ACHKV2_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO6938695.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19700..20368)
                     /gene="folE"
                     /locus_tag="ACHKV2_000761"
     CDS             complement(19700..20368)
                     /gene="folE"
                     /locus_tag="ACHKV2_000761"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO6938696.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20626..21462
                     /gene="yeiG"
                     /locus_tag="ACHKV2_000762"
     CDS             20626..21462
                     /gene="yeiG"
                     /locus_tag="ACHKV2_000762"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6938697.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21494..23473)
                     /gene="cirA"
                     /locus_tag="ACHKV2_000763"
     CDS             complement(21494..23473)
                     /gene="cirA"
                     /locus_tag="ACHKV2_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO6938698.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLAREDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23765..25234)
                     /gene="lysP"
                     /locus_tag="ACHKV2_000764"
     CDS             complement(23765..25234)
                     /gene="lysP"
                     /locus_tag="ACHKV2_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO6938699.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25439..26320)
                     /gene="yieE"
                     /locus_tag="ACHKV2_000765"
     CDS             complement(25439..26320)
                     /gene="yieE"
                     /locus_tag="ACHKV2_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO6938700.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26419..27468
                     /gene="yeiH"
                     /locus_tag="ACHKV2_000766"
     CDS             26419..27468
                     /gene="yeiH"
                     /locus_tag="ACHKV2_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO6938701.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27542..28399
                     /gene="nfo"
                     /locus_tag="ACHKV2_000767"
     CDS             27542..28399
                     /gene="nfo"
                     /locus_tag="ACHKV2_000767"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO6938702.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28402..29490
                     /gene="yeiI"
                     /locus_tag="ACHKV2_000768"
     CDS             28402..29490
                     /gene="yeiI"
                     /locus_tag="ACHKV2_000768"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6938703.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29546..30796)
                     /gene="nupX"
                     /locus_tag="ACHKV2_000769"
     CDS             complement(29546..30796)
                     /gene="nupX"
                     /locus_tag="ACHKV2_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6938704.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30896..31837)
                     /gene="rihB"
                     /locus_tag="ACHKV2_000770"
     CDS             complement(30896..31837)
                     /gene="rihB"
                     /locus_tag="ACHKV2_000770"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO6938705.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            31967..32665
                     /gene="yeiL"
                     /locus_tag="ACHKV2_000771"
     CDS             31967..32665
                     /gene="yeiL"
                     /locus_tag="ACHKV2_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO6938706.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32736..33986)
                     /locus_tag="ACHKV2_000772"
     CDS             complement(32736..33986)
                     /locus_tag="ACHKV2_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6938707.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34080..35018)
                     /gene="psuG"
                     /locus_tag="ACHKV2_000773"
     CDS             complement(34080..35018)
                     /gene="psuG"
                     /locus_tag="ACHKV2_000773"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO6938708.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(35006..35947)
                     /gene="psuK"
                     /locus_tag="ACHKV2_000774"
     CDS             complement(35006..35947)
                     /gene="psuK"
                     /locus_tag="ACHKV2_000774"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO6938709.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36371..38062)
                     /gene="fruA"
                     /locus_tag="ACHKV2_000775"
     CDS             complement(36371..38062)
                     /gene="fruA"
                     /locus_tag="ACHKV2_000775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO6938710.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38079..39017)
                     /gene="fruK"
                     /locus_tag="ACHKV2_000776"
     CDS             complement(38079..39017)
                     /gene="fruK"
                     /locus_tag="ACHKV2_000776"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO6938711.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(39017..40147)
                     /gene="fruB"
                     /locus_tag="ACHKV2_000777"
     CDS             complement(39017..40147)
                     /gene="fruB"
                     /locus_tag="ACHKV2_000777"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO6938712.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40515..41696
                     /gene="setB"
                     /locus_tag="ACHKV2_000778"
     CDS             40515..41696
                     /gene="setB"
                     /locus_tag="ACHKV2_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO6938713.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41693..41947)
                     /gene="yeiW"
                     /locus_tag="ACHKV2_000779"
     CDS             complement(41693..41947)
                     /gene="yeiW"
                     /locus_tag="ACHKV2_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6938714.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42102..42674
                     /gene="yeiP"
                     /locus_tag="ACHKV2_000780"
     CDS             42102..42674
                     /gene="yeiP"
                     /locus_tag="ACHKV2_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO6938715.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42897..44363
                     /gene="yeiQ"
                     /locus_tag="ACHKV2_000781"
     CDS             42897..44363
                     /gene="yeiQ"
                     /locus_tag="ACHKV2_000781"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6938716.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            44481..45467
                     /gene="yeiR"
                     /locus_tag="ACHKV2_000782"
     CDS             44481..45467
                     /gene="yeiR"
                     /locus_tag="ACHKV2_000782"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO6938717.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45506..46219
                     /gene="lpxT"
                     /locus_tag="ACHKV2_000783"
     CDS             45506..46219
                     /gene="lpxT"
                     /locus_tag="ACHKV2_000783"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO6938718.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46632..47198
                     /gene="mepS"
                     /locus_tag="ACHKV2_000784"
     CDS             46632..47198
                     /gene="mepS"
                     /locus_tag="ACHKV2_000784"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO6938719.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47379..48935
                     /gene="pdeN"
                     /locus_tag="ACHKV2_000785"
     CDS             47379..48935
                     /gene="pdeN"
                     /locus_tag="ACHKV2_000785"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6938720.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            49017..50831
                     /gene="yejA"
                     /locus_tag="ACHKV2_000786"
     CDS             49017..50831
                     /gene="yejA"
                     /locus_tag="ACHKV2_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO6938721.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            50832..51926
                     /gene="yejB"
                     /locus_tag="ACHKV2_000787"
     CDS             50832..51926
                     /gene="yejB"
                     /locus_tag="ACHKV2_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO6938722.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51926..52951
                     /gene="yejE"
                     /locus_tag="ACHKV2_000788"
     CDS             51926..52951
                     /gene="yejE"
                     /locus_tag="ACHKV2_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6938723.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52953..54542
                     /gene="yejF"
                     /locus_tag="ACHKV2_000789"
     CDS             52953..54542
                     /gene="yejF"
                     /locus_tag="ACHKV2_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO6938724.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54546..54890)
                     /gene="yejG"
                     /locus_tag="ACHKV2_000790"
     CDS             complement(54546..54890)
                     /gene="yejG"
                     /locus_tag="ACHKV2_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO6938725.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55223..56413)
                     /gene="bcr"
                     /locus_tag="ACHKV2_000791"
     CDS             complement(55223..56413)
                     /gene="bcr"
                     /locus_tag="ACHKV2_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO6938726.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56441..57136)
                     /gene="rsuA"
                     /locus_tag="ACHKV2_000792"
     CDS             complement(56441..57136)
                     /gene="rsuA"
                     /locus_tag="ACHKV2_000792"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO6938727.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57285..59045
                     /gene="radD"
                     /locus_tag="ACHKV2_000793"
     CDS             57285..59045
                     /gene="radD"
                     /locus_tag="ACHKV2_000793"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6938728.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59170..59454
                     /gene="rplY"
                     /locus_tag="ACHKV2_000794"
     CDS             59170..59454
                     /gene="rplY"
                     /locus_tag="ACHKV2_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO6938729.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59593..60600)
                     /gene="yejK"
                     /locus_tag="ACHKV2_000795"
     CDS             complement(59593..60600)
                     /gene="yejK"
                     /locus_tag="ACHKV2_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO6938730.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60782..61009
                     /gene="yejL"
                     /locus_tag="ACHKV2_000796"
     CDS             60782..61009
                     /gene="yejL"
                     /locus_tag="ACHKV2_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO6938731.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61029..62789
                     /gene="yejM"
                     /locus_tag="ACHKV2_000797"
     CDS             61029..62789
                     /gene="yejM"
                     /locus_tag="ACHKV2_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO6938732.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62864..62940
                     /locus_tag="ACHKV2_000798"
     tRNA            62864..62940
                     /locus_tag="ACHKV2_000798"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62898..62900,aa:Pro,seq:ggg)
     gene            complement(63041..65632)
                     /gene="yejO"
                     /locus_tag="ACHKV2_000799"
     CDS             complement(63041..65632)
                     /gene="yejO"
                     /locus_tag="ACHKV2_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO6938733.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            65952..66599
                     /gene="narP"
                     /locus_tag="ACHKV2_000800"
     CDS             65952..66599
                     /gene="narP"
                     /locus_tag="ACHKV2_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO6938734.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66634..67686)
                     /gene="ccmH"
                     /locus_tag="ACHKV2_000801"
     CDS             complement(66634..67686)
                     /gene="ccmH"
                     /locus_tag="ACHKV2_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO6938735.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67683..68240)
                     /gene="dsbE"
                     /locus_tag="ACHKV2_000802"
     CDS             complement(67683..68240)
                     /gene="dsbE"
                     /locus_tag="ACHKV2_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO6938736.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(68237..70180)
                     /gene="ccmF"
                     /locus_tag="ACHKV2_000803"
     CDS             complement(68237..70180)
                     /gene="ccmF"
                     /locus_tag="ACHKV2_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO6938737.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(70177..70656)
                     /gene="ccmE"
                     /locus_tag="ACHKV2_000804"
     CDS             complement(70177..70656)
                     /gene="ccmE"
                     /locus_tag="ACHKV2_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO6938738.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(70653..70862)
                     /gene="ccmD"
                     /locus_tag="ACHKV2_000805"
     CDS             complement(70653..70862)
                     /gene="ccmD"
                     /locus_tag="ACHKV2_000805"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO6938739.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(70859..71596)
                     /gene="ccmC"
                     /locus_tag="ACHKV2_000806"
     CDS             complement(70859..71596)
                     /gene="ccmC"
                     /locus_tag="ACHKV2_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO6938740.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(71638..72300)
                     /gene="ccmB"
                     /locus_tag="ACHKV2_000807"
     CDS             complement(71638..72300)
                     /gene="ccmB"
                     /locus_tag="ACHKV2_000807"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO6938741.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(72297..72920)
                     /gene="ccmA"
                     /locus_tag="ACHKV2_000808"
     CDS             complement(72297..72920)
                     /gene="ccmA"
                     /locus_tag="ACHKV2_000808"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO6938742.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(72933..73535)
                     /gene="napC"
                     /locus_tag="ACHKV2_000809"
     CDS             complement(72933..73535)
                     /gene="napC"
                     /locus_tag="ACHKV2_000809"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO6938743.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(73545..73994)
                     /gene="napB"
                     /locus_tag="ACHKV2_000810"
     CDS             complement(73545..73994)
                     /gene="napB"
                     /locus_tag="ACHKV2_000810"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO6938744.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(73991..74854)
                     /gene="napH"
                     /locus_tag="ACHKV2_000811"
     CDS             complement(73991..74854)
                     /gene="napH"
                     /locus_tag="ACHKV2_000811"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO6938745.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     VASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(74841..75536)
                     /gene="napG"
                     /locus_tag="ACHKV2_000812"
     CDS             complement(74841..75536)
                     /gene="napG"
                     /locus_tag="ACHKV2_000812"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO6938746.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(75543..78029)
                     /gene="napA"
                     /locus_tag="ACHKV2_000813"
     CDS             complement(75543..78029)
                     /gene="napA"
                     /locus_tag="ACHKV2_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO6938747.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(78026..78289)
                     /gene="napD"
                     /locus_tag="ACHKV2_000814"
     CDS             complement(78026..78289)
                     /gene="napD"
                     /locus_tag="ACHKV2_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO6938748.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(78279..78773)
                     /gene="napF"
                     /locus_tag="ACHKV2_000815"
     CDS             complement(78279..78773)
                     /gene="napF"
                     /locus_tag="ACHKV2_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO6938749.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        19244 a        21313 c        19506 g        18876 t
ORIGIN      
        1 aatatgtggc ttagctcatg aaaggagcgc aacaagatga tgaagctgaa taagcgattc
       61 attgtttctg ataaactgcg attccactat tatcgcagct taccgatatc aattgcctga
      121 atagcccttg caatatcagg ggaattattc aacacacgaa catgctgaaa taattctgtt
      181 gcttcatcgt attctttacg caaataactc aaccactgtt taatccgcgc tacgtgatat
      241 aacccggtat cgccctgctt ttccagacgg gtatattttt gcagcaaagc aaccacctcc
      301 ggccacggca ttcgcggttc gttatatttt actacccggc tcaggttggg aatattgagc
      361 gccccgcgac caatcatcac tgagtcgcag ccgctgatcg ccatgcattc ttgcgcgctc
      421 tgccagtccc agatttcgcc gttggcaatc accggaatat tcagccgctg gcgaatctcg
      481 ccaatcgcct gccagtcaat atgctccgcg cggtaaccct gctcttttgt ccgtccatgc
      541 accaccagct ccgtagcacc agcctgttga accgcatcgg cgatttcaaa tttcttctca
      601 ccgctgtccc agcccagacg cactttcacg ctgacgggca aatgcgccgg tacagcttca
      661 cgcatcgctt ttgcaccctg gtagatgagt tcaggatctt tgagtaacgt cgccccacca
      721 ccgctaccgt taaccgtttt cgacgggcag ccgcaattga gatccacgcc ccaggaacct
      781 aattccaccg cacgggcggc gttctctgcc agccattgtg ggaactgacc caacaactgc
      841 acgcgcacca gcgtaccgga tggtgtccgg ctggcgtttt gtagctcagg gcaaatgcga
      901 tgaaagactt tcaccggcag cagttgatcc accacgcgga cgaactcggt gatgcacaga
      961 tcgtagtcgt taacttcggt cagcaattca cgcaccagag agtcaagcac tccctccatc
     1021 ggagccagta acacacgcat atcatcaccc gcaaaaaaat gaggcgctat gttagcgcct
     1081 cctgtgtttg cagacaaact gacttactga ttaattcggg caagattttt accctcaccc
     1141 taaccctctc ccgaaggggc gaggggaccg cccgtgcgcc tgattgggtg aagaagtgct
     1201 tagcgtggtt gcaaacagtt atcttctttc cagccgtaca accactccat gccgcgataa
     1261 aattctgcct gcgatttccc tttccacagc agattacgta cctggcgttc tacccctgcg
     1321 gcgtggtaaa cccgccccat ttcacgggtc gaccagacga tgcgcgcggt gcgcggaatt
     1381 cgtaccgact catacagcgc aaaggcttgc gccgcatcac cctcacactg cgccagcgcc
     1441 ttacccagcg ttactgcatc ttccagcgcc atacaggctc cctgcgccat atattgcgcc
     1501 accggatggg ccgaatcgcc cacaagcgtt atgcgcttcg ttccccactt cgccaccggt
     1561 tcacggtcag cggttgacca gcgtcgccat gaagtcggtt tatccaacat ctggcgcgga
     1621 cgggggtgaa tcccttcaaa gtaagagagc acttcctctt tactaccatc cttcacgccc
     1681 cactcttcct gctggcggct gtggaatgtt accaccaggt tatactgctt gccgccgcgc
     1741 aaagggtaat gcaccaagtg acaatgcggc cccgcccaca gtaccggtgc gttaatgcgc
     1801 aaatcttccg gcatatcggc acgatcgata accgcacggt agacaacatg tccggtgaca
     1861 cgcggcgcat cgcccaataa gctctggcgc accactgact tcacgccgtc acagccaatc
     1921 agaatatcgc cggtccagct attgccgcga tcgtcaaaca cggtgacatc gtcttccgtc
     1981 tcgcgaatat ccacgacgtt ggtggaggtg tgatattcca cgtccggatg cgtcagtacc
     2041 gcttcccaca ccgaggcgtg aatatcaacc cggtggatca ccgcgtaagg gccgccgaaa
     2101 tggtcacgaa aggcctgccc ggtttcaatg cacaccactt cttcggcgtt caccgcatcc
     2161 atcatggtaa tgtgatcggt aaacaccgcg cgctggcggg caacttcgcc gacgccgagg
     2221 ctgtccagcg ccgaaaacgc atttggcccc aactggatgc ccgcgccaat ctcaccgatc
     2281 tcatgcgcct tttccagcag caccactttg atccccaggc gggccagtga cagcgcagtt
     2341 gccgcaccgc cgatcccgcc tcccacaatt actgcgcgtg tcactttcgt cattatagtt
     2401 ctcctggtca ggctgaaatt ttatcttgtt gattttctgg cgctgcggca atgaacgctg
     2461 gcagctgtgt gcaggcgtcg tacaccgctt tgcagcgtgg atacccgctc aaatcgcagt
     2521 tcatgcgtag cgcgttcgcc cactgcggga caaggcagca atctgccagc gtcggcgtct
     2581 cgcccacaca aaattgcccc gactggttct ggcgcaacag ttgttccacc gcgcttaacc
     2641 cttgctggat ccagtgcgca taccaacgtt ttttttcttc ttcactgacg ttcagttctt
     2701 cggtcaggta gcgcagcacg cgcaagttat tcaatggatg aatatcacaa gcgatggcat
     2761 agacaatttc cagcaccttg ttgcgggccg gctcttcctg cggcaccagc ggaacttgcg
     2821 ggtagtgcct ctccagccag tcaataatcg ccagcgattg tccgagtgat tcgccctcgt
     2881 cagtcagcag cgtcggcacc agcccgaccg gattcatccg ccgataggcc agttcatttt
     2941 gctgaccaat acggatattc accccttcgg tctggtaatt aatgcctttc agcgccagcg
     3001 caatgcgtac ccggtacgac gctgaactat taaaaaaact gtacagcttc atcatgcacc
     3061 tcagacaatc ttcaccgcga taggcgttaa gccctcgacg ttgcctgtga tcacatcacc
     3121 tttcactacc gggccaaccc cttccggcgt accggtgaag atcagatcgc ccggttgcag
     3181 ctcaaagaag cctgacagat agcagatggt ttcattcacc gaccagatga gatggcgaat
     3241 atcgctgcgc tgatgatctt cgccgttaac ctgtaaccag attggcgcat tatcaatacc
     3301 gccgatatcg cgggctttgt gcagcggcgc gatgggcgct gagagatcaa acgctttacc
     3361 gatttcccac gggcggccca tctggcgcat ttccatttga cggtcacgcc gagtcatatc
     3421 aagacccgtg gcataacccc agacatattc atgggcgtgt tccagcggga tatcgctgcc
     3481 ttttttacca atcgccacca ccagttcaat ttcgtaatgg tagttatcgg tctgtgccgg
     3541 atacggcagc gccagcgttt caccagcggc aaccggaaca acggcatccg ccggtttgca
     3601 gaagaagaat ggcggttcac gatccggatc aaagcccatt tcgcgggcgt gagcggcata
     3661 gttacgtccg acgcagtaaa cgcggcgcac ggggaattgt tcatcactgc caacaaccgg
     3721 tacggtaacg ggcgcttgcg gttcgaatac gtattgagtc atttttctct cctaaattaa
     3781 taacgcgctt cgcggaacag ccccagggct tcttgtaccg gtctgtccga aaaactaaat
     3841 aaaacggaat cttgcgtggt ctggaacgac acgccatgcc aggtcggcac cacgaagata
     3901 tcttttgcac taaagctgaa ggtttcattg ccgatgatga cctgcccgct accttccacc
     3961 acatggtaga tggtgctgtc agtggtacgc gcaacgcgtg aggcaaaccc tttcggcagc
     4021 agttgcagga aagcgcccat cgacggcatc ggatagccgc cggtgaccgg gttgacgtag
     4081 cgcattttgt agccatccca ctcgtcggca tcgcccagtc gggttaaatc gtgcagcact
     4141 tcgcggctgc ggtcataacg atagttaaag atcggcgagg agttccccgt ctgatgccgc
     4201 agcggcaaca tattggcagc gtaacgcggc agataatctc cctctttacg cgttaccggt
     4261 tgttgctctt ccggataatc ttcggcaaaa ccgcagccca gaatattcac taatggcaga
     4321 tccagcccat cgagccagat aaccggttcg tcgccagggt taccgtgatc gtgccagcgc
     4381 cactgcgggg tcaggataaa atcgccttca ttcattggcg tgcgttcgcc atcaacggcg
     4441 gtaaatgccc ctttgccttc aacaataaaa cgcagcgccg actgattatg acgatgactc
     4501 ggtgccactt cgcccggcat gatcagttgc aaaccggcat ataaagtcgc ggtaatggaa
     4561 gactgcccgc gcagcgccgg gttttccagc accagcacgc ggcgcacggc ctctttcgca
     4621 ccaatcaggc cgccgctctc cagtagcagc gggcggatct cctgataatt ccagtacgct
     4681 ggcgcacagt tagcgttggg cgttttcggc accagatggt gcagtgactc ccacagcggg
     4741 gtcaggtttt gcccggaaat atgctgataa aattgctcgc ggctattttg attgttatca
     4801 gtcattgaga tgctccttat tcgttcacaa cgcctgcgcg aggcagtgag tcagggacag
     4861 aggtcgactg gcgaaccacg gtaatcagca gggttaacat caccgcactg atcgccgcag
     4921 gcacagcgat aatgaaaaac agggtgtcga aagagaaatt catcgccatc atcacgccgc
     4981 cagacagcga accaacaatc gcgccgcagc ggccgatagc atttgaccag ctcacgcccg
     5041 tggctcgact ttgtgttgga taaagtgttg cggtcagcgc attcagcccc acctgcgatc
     5101 cgctaatgcc aatgccagtg ccgaaaatcg ccagcgccat caaccataat ccatcctggc
     5161 ttaagccaat catcacgatg catatagcgc ctatggcata gctcagcgtc agcacgcgga
     5221 aaggattgaa cttatccatc agcacaccca gcgccagcgc gcctaacgta ccaccaatct
     5281 ggaacgcggc ggtgacccag gacgcgtgtt gcaggtcgat gccgcgatgg ttcagtagcg
     5341 tcggcatcca gcttgagagc aggtaaataa tcagcaggct cataaagaac actacccaca
     5401 gcatcaacgt gatgggtaac tgacgtccca taaacagttg acgaatgctg ccttttgtaa
     5461 cggaggctga ttcaatgaga taaaagtggg tatcgacata acgctcgcgg gtgatggcgc
     5521 tgacggtttt cgcaatcacc gcctgcggta gctggcggcg cacctgccag cgtggcgatt
     5581 ccggcagaac taccagcagt gcgacaaaca gcatcagcgg taacacgccc cccaacacca
     5641 gaatgccgtg ccagccaatc accggcacca gctgcgcact gacaatcccg ccaaacgccg
     5701 aacccagcgt aaagccgcaa aacatcagcg tcaccagcgc gccgcggcgg cgggcaggca
     5761 gatactccga ggtcatggtg atggtgttcg gcatcgcgcc gcccagaccc aggccggtga
     5821 gaaagcgcag gaagaccagc gtttgcagat ccggtgagaa agccgaggcg aggcttaacg
     5881 cgccaaacaa aaagacgcac agctcaatca cccgcttgcg accaaagcga tcggacaacg
     5941 ggccacaaag cagtgcgcct gcggttaatc ctagcagacc cgcgccaaaa agcggggcga
     6001 gatcgcctgc ggtgagttgc cagtgagtgc gaatatcagg ggcgataaat ccgatagccg
     6061 cggtgtcaaa accatcgagc attaccacca gaaaacagca gatgataacg cgccactgca
     6121 tcttgccgac cggagcagca tcgataagtg cttgtaaatc acgtcgttga gtcatagtaa
     6181 atgcctcagt gcaggtaagg tagatgtttc ttttgttttg tatatgttgc gatattgtga
     6241 ttaaagagat gagatgtaca atggcatttc gggcagagag ataaccttga ggttatggca
     6301 aatggcgaac tgggcgcaaa aattaaaact acatcacctt gaaatgctgg tggcgctggg
     6361 tgaacagggc aatctaaccc atgtcgcacg aatgatgaat atcacccaac ctgcgctttc
     6421 gaaatggttg tcacaactcg aagatgagat tggaatcaca ctttttgagc ggcacagcaa
     6481 aggattacgt ccgactgaag gcggcaaatt gttattgcaa catgcccaac ggttaatcaa
     6541 tgacatggag cgatcgcaac atgaactggc ccgttttaaa caaggtggac tggtcggcag
     6601 tctgaaaatt ggctgttctc ccgtcgccac tgactgcgta tcccaggcga ttttgcattt
     6661 gcttacgaag atgccaactc tacacctgaa tatcgaagag aaggtgatga ccccattgct
     6721 gcaagatcta ttggccggtt ctgtcgatgt ggtcgttggg cgaattggcg gtcgcgcatt
     6781 gcagctgcca ctgaattatc aggtgctgta caccgagcca gtatgctttg tcgcccgccc
     6841 tgagcaccca ctggcgaaat acgccaccct cagctggaat gatcttgcca actggcgctg
     6901 gatcgtctgg ccgacgggta cccccattcg cattagtatt gataatgcgc tggtagacca
     6961 cggcgtaatg ctgccagaaa acacgattga gtcggcgtcg atgaacgtca gtaccaacct
     7021 gttacaaagc agcgatatga tctctatttt gtcattacgc ctggcgcaac gttatgccag
     7081 ccagggacaa ctggtgatac tcaacctgcc aaagatagaa cagaaaggta gcgtcggtgt
     7141 gttctggcgc aataacgaaa cgccttctta tgctttaagt cagtttctgc actttttaca
     7201 gcagaattag tgtgctgaac ggggcgctcg cccgaggtta cctccctaaa attcaactat
     7261 gctgagaatt cttaatgtga tcggtagcac gttttacgaa ttaattgtat gatgaatcca
     7321 tctcatctgg ggtgttgatt atgagtaaga cactgaacat tatctggcaa tatttacgcg
     7381 ctttcgtcct gatttatgcc tgcctgtatg caggcatttt cattgcttcc ctgctaccgg
     7441 taaccattcc gggcagcatc atcgggatgc tgatcctgtt tgtcctgctg gccttgcaaa
     7501 ttcttccggc gaaatgggtc aatccggggt gctacgtact gattcgctat atggcgctat
     7561 tgtttgtgcc gattggcgta ggcgtcatgc aatattttga tttgctccgc gcacagtttg
     7621 gcccggtagt ggtttcctgt gcaatcagta cgctggtggt ttttctggtg gtgagctgga
     7681 gttcgcaact ggtacacggt gaacgtaaag tcgtaggtca gaaaggatca gaagaatgat
     7741 ggcgaatatc tggtggtcat taccgctgac gttgattgtc ttttttgccg cccgcaaact
     7801 ggcggcacgg tataagtttc ccctgcttaa cccgttgctg gtagcaatgg tggtgatcat
     7861 tcctttttta atgctgaccg gcatctctta cgacagttac tttaaaggta gcgaagtgct
     7921 aaacgatctg ctgcaaccgg cggtcgtcgc gctggcctat cctttatatg agcagctaca
     7981 ccagatccgc gcgcgctgga aatcgatcat caccatctgt tttatcggca gcgtggttgc
     8041 gatggtgacg gggacttccg tggcattgtt gatgggcgct tcaccggaaa tcgccgcgtc
     8101 aatcctgccg aaatcagtca ccacgcctat tgcaatggcg gttggcggca gtattggcgg
     8161 tattccggca atcagcgcag tttgcgtgat tttcgtcggc atcctcggcg cggtatttgg
     8221 ccatacattg cttaatgcga tgcgtattcg taccaaagct gcgcgcggtc tggcaatggg
     8281 gactgcctcg cacgccctcg gtacggcgcg ctgcgccgag ctggattatc aggaaggtgc
     8341 atttagttcg ctagcgctgg tgttatgcgg gataattact tcgctgatcg caccgatcct
     8401 tttcccgatt attctggcag taatgggcta aaatttgcga tgcgtcgcgc atttttgatg
     8461 taagtttcac gcgttgcata attaatgaga ttcagatcac atataaagcc ataacgggtt
     8521 cgtaaactgt tatcccatta catgattatg aggcaacgcc atgcatccac gttttcaaac
     8581 cgcttttgcc caacttgcgg ataacttgca atctgcactg gaacctattc tggcagacaa
     8641 gtacttcccc gctttgttga ccggggagca agtctcatcg ctgaagagcg caacggggct
     8701 ggacgaagac gcgctggcat tcgcactact tccgctggcg gcggcctgtg cgcgtacgcc
     8761 attgtcgaat tttaatgttg gcgcaattgc gcgcggtgtg agcggaacct ggtattttgg
     8821 tgccaatatg gaatttattg gtgcgacaat gcagcaaacc gttcatgccg aacaaagcgc
     8881 gatcagccac gcctggttga gtggtgaaaa agcgcttgca gccatcaccg ttaactacac
     8941 gccttgtggt cactgccgtc agtttatgaa tgaactgaac agcggtctgg atctgcgtat
     9001 tcatctgccg ggccgcgagg cacacgcgct gcgtgactat ctggcggatg cctttgggcc
     9061 gaaagatctg gagattaaaa cgctgctgat ggacgagcag gatcacggct atgcgctgac
     9121 tggtgatgcg ctttctcagg cggcgattgc ggcggcaaac cgttcgcaca tgccttacag
     9181 taagtcgcca agcggtgtcg cgctggaatg taaagacggt cgtattttca gcggcagcta
     9241 cgctgaaaac gccgcattca acccgactct gccaccgttg cagggagcgt taattctatt
     9301 gaatctcaag ggttatgatt acccggatat ccagcgcgca gttctggcag agaaagccga
     9361 tgcgccgttg attcagtggg atgccacctc cgcaacactg aaagctctcg gctgtcacaa
     9421 tatcgaccgc gtgcttctcg cttaagcctg gtgccggatg cggcgtgaac gccttgtccg
     9481 gcttgccagc cctctcctgg tgtcgaaaat cccggcaaac agtttgtcgt ttcttgcgca
     9541 aaaccagcgg gtaaagtagc ctgatggaaa ttttccttag atcgagtctc ctgcatgtta
     9601 aagcgcgtgt tcctcagcct gttagtcctg atcggcttgc tgctgttgac tgtgctcggc
     9661 ctcgatcgct ggatgagctg gaaaaccgcg ccttatatct acgacgaatt gcaggatctc
     9721 ccctaccgcc aggtcggtgt ggtgctcgga acagcaaaat attatcgtac tggcgtaatt
     9781 aatcagtatt atcgctaccg cattcaagga gcgattaatg cctataacag cggtaaggta
     9841 aattatctat tactgagcgg cgataacgca ttgcaaagtt ataatgagcc gatgaccatg
     9901 cgcaaagatt taatcgctgc tggtgtcgac ccatcagata ttgttctcga ttacgcaggc
     9961 tttcgtacgc tggattccat cgtgcgtaca cgcaaagttt tcgatactaa tgatttcatt
    10021 attatcaccc aacgtttcca ctgtgagcgg gcattattta ttgcgctgca tatggggatt
    10081 caggctcagt gttatgccgt accgtcaccg aaagatatgc tgtcagtacg tattcgtgaa
    10141 tttgccgccc gtttcggtgc gctggctgac ctttatattt ttaaacgtga accgcgtttt
    10201 ttagggccgc tggtccctat tccggctatg caccaggtgc cggaagatgc gcaggggtat
    10261 cccgccgtca cacccgaaca gttacttgaa ttacaaaaga aacaaggaaa gtagttatgg
    10321 atgtacagca gttttttgtc gttgccgttt ttttccttat cccgatattt tgtttccgcg
    10381 aagcatggaa aggctggcgc gcaggcgcga ttgataaacg ggttaaaaat gcaccggaac
    10441 cggtgtatgt ctggcgagca aaaaatcccg gactcttttt cgcttatatg gtggcatata
    10501 tcggcttcgg aattttatct atcggcatga ttgtttatct tattttctat cgttaatatt
    10561 ttctcttttc tgttaattat tgagaataat tattacttca ctcaataagc tgcggatata
    10621 acattctgaa ccgcagctat ttgtgaatcc tttcacagtt taaatttccc cacgcgcata
    10681 gccttaatat cagcacgcaa tatttattta gcgttcgcct taattactca tagcattaag
    10741 gaagatcaca tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgccgtta
    10801 ctggcgatta aagaagcctc tcgctgttta ttatgtcacg acgctccctg tagtcaggct
    10861 tgcccggcac agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa
    10921 ggcgctgccg agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc
    10981 ccgacggaga aattatgcca aagcggttgt acccgtgccg gtgttgatgc tcccattgat
    11041 atcggccgct tacagcgttt tgttactgat tttgaacaac aaaccgggat ggaaatttat
    11101 cagcccggta ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg
    11161 caggccagtg tgacgctaac aaaccagggt tatgacgtca cgatttatga gaaagaagcg
    11221 caccccggtg gctggttgcg taacggtatt ccgcaatttc ggttaccgca atcagtgctg
    11281 gatgcagaaa tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa taacgaaatc
    11341 ggtaaaacac tcaccctgga gcaactgaaa gcggaaaacc gcgcggtact ggtcaccgtg
    11401 gggttatcaa gcggttccgg gctaccgctg tttgagcata gcgacgttga gattgccgtc
    11461 gacttcctgc tacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta
    11521 attatcggcg gcggtgatgt cgcgatggac gtagccagca cgctgaaagt tctcggctgt
    11581 caggcggtaa cttgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag
    11641 tttgccagcg cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg
    11701 gaaggcaata aagtcacgtt taagcatgta cggttatcgg gcgaactgac gatggcggca
    11761 gataaaatta ttctcgccgt cggtcagcat gccagactgg atgactttgc gaagttagag
    11821 ccgcagcgta acaccatcaa aacacaaaat tatcagaccc gcgatccgca agtctttgct
    11881 gctggcgata ttgttgaggg tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa
    11941 gccgccgagg cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt
    12001 cgattacttt ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgccggtag
    12061 gcaactgcta tgagatgtgt gccaaagcct acgacacagg ttggggcggc gtggtgttta
    12121 aaacgatcgg cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag
    12181 aagataccgg ttttatcggc ttcaaaaata tggaacagat tgctgaacat ccgttggaag
    12241 agaatctggc cgccctgcgt cggctgaagg aagattaccc ggacaaagta ttgatcgctt
    12301 cgatcatggg ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg
    12361 gcgcggatat gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta
    12421 gcgatgtcgg gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt
    12481 ccacgctgcc aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc
    12541 tggcggcgaa gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca
    12601 ccaatatcga tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcgagtattt
    12661 ccggatattc cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag caaatgcgca
    12721 cccatccaga actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg
    12781 atgcggcgga gtttttattg ctcggcgcag caacgttaca ggtcaccacc ggcattatgc
    12841 agtacggcta tcgcatcgta gaagatatgg cgagcgggtt atcgcattat ctcgccgatc
    12901 agggatttga ttcgttgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg
    12961 aagatttaga ccgcagttat attgtctatc cccgtatcaa tcttgataaa tgtgttggct
    13021 gtggacgctg ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga
    13081 aaacccgcac accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg
    13141 tctgcccggt gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac
    13201 acccggtaac gttgtaaaac cagagacgca tccggcattt ggttcctgtc gccggatgcg
    13261 gcttgaacgc cttatccggc ctacaaaacc atacaaatca gtatgttgag ctcattgcag
    13321 gccggataaa acgcgccagc gttgcatccg gcatttggct cctgtcgccg gatgcggcgt
    13381 gaacgcctta tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg
    13441 aatgcggacc acaatcattt cacttcttac gcgcgtactt cagtgaatcc agcgctacgg
    13501 cgaagataat aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatgtacg
    13561 tcaggccata gttgatgacg gtaaaaataa ttaccccggt caccacgcca atcaccgtcc
    13621 ccacaccgcc gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagttcat
    13681 acataaagcc gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa
    13741 aggcatagaa cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac
    13801 cagatacttt tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg
    13861 ttttgttcca caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag
    13921 agagacggaa actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa
    13981 aaccagaaat tggcgacgcc ccgacaaagt cgtaataaag cgagttgatg ccatagacga
    14041 tgatcatcgt gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac
    14101 cgttgatcaa accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg
    14161 gcatcgtcgc catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg
    14221 ctgccaccac tgccgccagc cctacttgac gccccgcaga aaggtcggtc ccctgggtga
    14281 caattaaccc tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat
    14341 tacttaagtt caacagactt aaaaatgttg gggcctggaa aataataatc gcaagcaaaa
    14401 ctaaaagaac gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta
    14461 acgcactcat gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgt agaatttcgt
    14521 tttgcgttgt tgtttttgta tcgacaattc cggaaacgag gccattgctc ataaccagaa
    14581 tacggtctgt tatccctaac aactctggca tttcagagga gataataata atccccttgc
    14641 ctttcttcgc cagttcggca attaactgat aaatctcaaa cttcgcccca acatcaatac
    14701 cgcgcgtcgg ttcatcgagc attaatattt ctggttgcgt taacagccag cgcccaataa
    14761 tcaccttttg ctgattacca cctgagagcg aaccaatttg cgtccgatgc cccggtgttt
    14821 ttacccgcat cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta
    14881 aaccaacttt atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca
    14941 gataggcata aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta
    15001 tggcttcgtt ggcattatgg ttattgatct ttttgccgtg caacgtaatg gtgccagccg
    15061 atttctcgcg aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagac
    15121 cggcaatacc gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct
    15181 ggcgcagtga cgtcaggtta cgtacctcga ggatgacttc gcccggcttg ttttctttgt
    15241 caggaaaacg ctggttaaga gaacgcccaa ccatcatggc gataatcttg tccatcgtca
    15301 gccctgccag cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac
    15361 ataactggaa gatttcctcc attttatgcg agatataaac aataccgcag ccgcgctctt
    15421 ttaatttacg aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg
    15481 gttcatccat aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct
    15541 gcatttgcga aacagataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt
    15601 catcaaaaat cgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg
    15661 tggggtatcg cccaagccac atgttgtcca tcaccgaacg ttgtaatacc agatttaact
    15721 cctggtgtac catcgaaata ccattttcca gtgcttcttt ggccgaatgg aaatcgatct
    15781 ctttaccctg gaataaaatg gtgccggagt cttttttata aataccaaac aggcatttta
    15841 ataatgttga ttttcctgcg ccgttttccc ccattaatgc atggatagaa tgtggccgga
    15901 cttttaaatt aacgttatca agtgccttaa caccaggaaa agacttgttg ataccgctca
    15961 tttccaacaa gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg
    16021 ttggccatac aataagggcg cagtaataga ctgcgcccaa tcagtcttat ttcttgctga
    16081 attcagccag gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga
    16141 ttttccagtt ggtgccatca gccgcacctt taccatcggc caggtttttc gccagatcaa
    16201 aggtcgcttt cgcctggttg ttagcatcgt tcagtacggt gcccgccagt gcaccggatt
    16261 tcaccagcgc cagcgcttct ggcagagcat cgacgccaaa caccggaatg ctggacttgt
    16321 tgtgtgcttt cagtgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca
    16381 cttcgatttt gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag
    16441 cggtatccca catagcggta tctaactgta actgttcagt tttaatgcct ttgtcgttca
    16501 gctctttaat cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca
    16561 gcagtacgaa ttgaatctga ccgtctttat tcagatccca accctgattc gccgcccagt
    16621 gtttagcaat caaatcgcct tgaataatgc cggactcttt ggagtcagtg ccaacgtagt
    16681 aggctttgtc gtagctatcc agcgccttac gagacggttc tttgttaaag aataccaccg
    16741 gcacattttg cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt
    16801 tgattgccag tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg
    16861 actggtcatt ctgtgaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt
    16921 gctcaatagc cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac
    16981 caatgcgagt atcagcagcg tgtgcagtgg caccgaataa catgctggcc atcacagcag
    17041 acagggttaa caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt
    17101 gagataaatg ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac
    17161 tttttaaaat tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt
    17221 atgacgcatt acctcggtga aagtgattat tcactgttac atacgggtta caacgttaaa
    17281 acggtgcaat catagctatc acattgttaa gatactgtga aatcactcac agattgaaag
    17341 cggttacatc gcctgatttg ttgagttagt gatcgccgcc gcattctggc gtgttgcgac
    17401 agaatggcga cgcactaacg tcggcataaa acagtggctg gcacgaggat caatattgcc
    17461 tgctgccccc tgcaaggcca gttcggtggc taatttcgcc attgaagcaa tgggataacg
    17521 cacggtcgtt aattgcgggt cggtgtaacg ggcgatggga atatcatcga aaccgatgat
    17581 tgagagatgt aacggaatcg caatgccatt atcttttaat gctgtcagtg cgccagcagc
    17641 catattgtcg ttataagcaa atacagcggt aagttgtaga ttgcgcccca gcagttcaac
    17701 catcgccgcc tcaccgcccg gcatgtccgg cgtaccagtg ccaatccagc tttccggcgg
    17761 aataatatcc tgctctttca acgcactcat ccagcctgct ttacgcatgg cgtcatcttc
    17821 aatgccgtgg ctggaagaaa gataaccaat acgttgatga ccgttattca gcaacatgcg
    17881 cgtcgccatt cgggcaccgc tgagattatc caggcaaacg caacgatggg tgtaccccgg
    17941 cacaacgcgg ttgattaaca ccataccggg aatgttatcc ataaattgcg ccagttcatc
    18001 gtcactcaat gcttttgagt gaacaatcaa cgcattacaa cgctggcgaa ttaacacctc
    18061 aatggcgtga cgctcttttt ccgcttcatg atagctattg ccgattagca cgtatttctg
    18121 atgctgctga gcgaccagat ccaccgcttt taccagcgcg ccgaaaaacg catcagaaac
    18181 gtccatcacc accacgccaa tggtgtcgct aacctgagtt gccagcgcct gggcattggc
    18241 gtttggccga taatccagct cactcacggc tttcattact gcttcacgcg tgtcggcact
    18301 gaccagcgtg ctgttattga gcacccggga aaccgttgcc acagagacgc cagcctgacg
    18361 cgctacatca cgaatggtga tcatattcac tacctgttat gagattgcag taaatgactg
    18421 cttgctgccg gctattttgt cagcactgaa tacaggactt cgtgaatcga gtcacagcaa
    18481 tggaaacggt tacagccgtt ttgttaacga atgtgatcca gatcgttatc ttgatgtttt
    18541 agatattgcc ggtccggcag cacgcaaagt taactgacgc cagagccatt ccaccggccc
    18601 ctggcggaag taacgcagcc agataacaga gaagagaata ttcgccagcc ataccggaat
    18661 aacaaacgcc agcaattcca gacggtcaaa ctgcataaac agaccgaggt ggtaaaaaag
    18721 cgtggtacaa atcagcgttt gcaatagata gttggtcagc gccatccgtc cgacgcaggc
    18781 gatcgccagc accagcttaa agcggctcaa ttgcggccag aagccataaa acagcgacgc
    18841 atagccaatc gcctgaaacg gcgcactcag ttcccgcggc atttgaagta agaaggcaca
    18901 ccagcgatac gcccagtcca gctgccattg cagggcgatg gcaggaaggt taatgatcac
    18961 cccaatcgcc actagcacaa aaccagtacg acgatagtga cgtaagctga actgcccttt
    19021 cagccagcca ctgcgcatca atgcggcacc aatgagcatc atcccagcca gttgccagcc
    19081 atactgtgcg cccagtgcca ataaactgtt cccaacacca tcggcacgat tactgatcgc
    19141 ttcaacgccg ccgtgaagct tccagtattt ttcatataaa atagccgatg catccggcgt
    19201 ccaggcgcgg ctagtctggc tatcggaaat caaccccaat aacagcaaaa cgccaagccc
    19261 caccagataa agcatgacgc cggtattaaa aaggcttttt accgatggcg catcgcgcac
    19321 cagccgccag cagattaagc ccaccagccc gtaagccagc agaatatcgc cgtcccagaa
    19381 caataaaccg tgaataaagc ccagcaagac taacagcgtt aaccgcgact ggatccagcg
    19441 tctgccacgg ggcagcaaca tttgcaggcc agcaccaaac agcagcgcaa aaagcgtgag
    19501 gaatttcacc tggccgatga gatcgagaaa tgcccaggtc catgcatcct gcggcgtaat
    19561 agcgccgtac caggcgggat taagataagc cgcctttggt agcccaaagg cgctgatgtt
    19621 taatagcagg atccccagaa tggcaacgcc gcgaacaaag tcgagcgtga cgttgcgctc
    19681 catggttcct gcctttaaat cagttgtgat gacgcacagc gcgcagaaac tcgtggcgcg
    19741 tattctgact ggatttgaac aatccaccaa gagaggtcgt tgtcgtggca ctggttgcat
    19801 cgcggatgcc acgcgccttc acgcaataat gcaccgcgtc gatagagaca gccacgttat
    19861 tggtgcccag cagcgtttgt agcgcaatga gaatttgctg cgtcagacgt tcctgcacct
    19921 gcggacgctg ggcaaagaac tgcacaatac ggttaatttt tgacagaccg atcaccgaat
    19981 ctttcgggat ataagccacc gtcgctttgc catcgatggt aacaaaatgg tgttcacagg
    20041 tactggtcag agtgatatcg cgcacggtga ccatttcatc gaccttcatt ttgttttcaa
    20101 tgagggtgat tttcgggaag ttggcgtaat ccagaccgga gaaaatttca tcgacataca
    20161 ttttagcgat gcgatgcggc gtttccatca aactgtcatc agccaggtcg agattcagca
    20221 gctgcatgat ttcggtcata tgaccagcaa taaggctttt gcgcgtttcg ttatccattt
    20281 catgcacggg cgggcgcagc ggtgtttcca gtcctcgcgc aactaacgct tcatgaacca
    20341 gggccgcttc tttactgagt gatggcattt atgatttctc ctgcaggtgt gacgcctccg
    20401 ccctgcgtgg gggcaaagtt attaagctga tttacagcct gattattgtg cgtgaggcgg
    20461 cgcacataat ccagtattca cagcgataat tattgtaatt gccgctgcct ttcatcagca
    20521 gatgttaaaa catcgttatg caaatacgga agtgaaagtt actcacagca cattgaataa
    20581 acggtatgat gaagaaattg caaacaacac aacaaggagc cacgcatgga aatgctcgaa
    20641 gagcaccgct gttttgaagg ctggcagcaa cgctggcgac acgactccag taccttaaac
    20701 tgcccgatga cgttcagtat ctttctccca ccccctcgtg atcacacccc gccaccagtg
    20761 ctgtactggc tttccggatt aacctgcaat gacgagaact tcaccaccaa ggcgggtgcc
    20821 cagcgcgtgg cggcggaact ggggattgta ctggttatgc cagacaccag cccgcgcggc
    20881 gaaaaggttg ccaacgacga tggctacgat ttaggccagg gcgcaggctt ttatcttaat
    20941 gccacgcaac cgccgtgggc gacgcattac cggatgtatg attatctgcg tgatgaatta
    21001 ccggcgctgg ttcagtcgca atttaatgtc agcgaccgct gcgctattag cggtcattca
    21061 atgggtggtc acggtgcgct gattatggcg ctgaaaaacc cgggcaaata caccagcgtt
    21121 tcggcctttg cgccaattgt gaatccgtgc agcgtcccgt ggggaatcaa agcgtttagc
    21181 cgctatttag gtgaggacaa aaatgcatgg ctggaatggg acagttgcgc gctgatgtat
    21241 gccagtaacg cgcaggatgc gatcccgacg cttatcgatc agggtgataa cgatcagttt
    21301 cttgctgacc aactgcaacc tgcagtactt gcagaagccg cgcgccagaa agcatggccg
    21361 atgacgctgc gtattcagcc gggatatgat cacagttact acttcatcgc ctcttttata
    21421 gaggatcacc tgcgcttcca tgcgcagtat ttactgaagt gaaagtccgc ccggttcgcc
    21481 gggcatcttc tcatcagaag cgataatcca ctgccataaa gtaacgacgt ccgtcttcgt
    21541 tatagctgta gtcgtcacga ctgagatcct tgtcgccaag gttcagcacg cctgcgcgca
    21601 gtttgacgtc tttagtcacc tgccaggccg cgccggtatt ccagatggta taaccgcccg
    21661 gtgttttagc cgtcgcgctg tcggctcgtt tctgcccggt atagtgacca gaaacataga
    21721 atgaccagtc ttcccgcgcc agcggtttcc agtccagcgt accgttagcg gtatggaacg
    21781 gcagatcgga tagcggtttg ttttcgccgt tgctgacatc acgaccatcg ttgtaggtgt
    21841 agttgagtga cagtttccat tcatcgttga acggaatttt cagctcggtt tccacgccct
    21901 gaatacgtgc tttgttaacg ttgtagtagc taaataccgg cacaccgttc gctgtaaaac
    21961 caacaaagtt ttggtagccc ggtgcagcat tgacgtcaga cgtacggctg atgctgatac
    22021 gatctttcac atcgttacgg aaaacggtaa cgctggattc aaccccttcc agccagcctt
    22081 cttcacccat gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg
    22141 ggctacccac aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt
    22201 gcaacagaga aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg
    22261 tggcgttata aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt
    22321 ccatacgcac gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa
    22381 acagcgcgta ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat
    22441 cgctaagttt gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca
    22501 gcggcaacgt gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc
    22561 cagggttttt gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc
    22621 aacgcccatt atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt
    22681 cggaatcacg atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg
    22741 gtgtccaggc aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt
    22801 ctccggtatc ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc
    22861 cgtaagcttt cattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt
    22921 tataggtatc accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg
    22981 accatttctg accgattttt ttggtgatga tattcactac accgccgagc gcatcggaac
    23041 cgtacagcga cgacatcggg ccacggacca cttcaatacg ttcgatggaa tcgaccggga
    23101 tccagttcag atcgaaatca ttgtggcgga agacggcatt gcgggagtta acgcgtttac
    23161 cgtcgacaag aatcagggtg tagctgctgt ccagaccacg aatactaacg cccttacggt
    23221 tatccccttc gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct
    23281 gtaccggttt tcgctgcagg tcttcctggg taatgacgct gatactggcg ggtgcatctt
    23341 taaggttttg ttcaacggaa gatgcagtga caaccatcgt ttcgccatcg tcatcgaccg
    23401 ctaacacagg ccatgcacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt
    23461 tcaacctaaa cattccatat ctccatgagg taactacgaa aataaaatgg gttatcgctc
    23521 acatcttctt cacgtcccct tgcgtacggc agcatcgcgg tggacttatt tgtatgcagg
    23581 tgatcatccc agaaagccgg accagcctct gatttgtcag ccttttttga ttgcggcgta
    23641 acgataatgc aaacgataac aattatcaat ccaaatgtta aattttatat ccgcatgatt
    23701 tgtcagggaa atggaattga aaaagccctc tcggttgaga gggcttagca aggaagggag
    23761 aaacttattt cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgggttc
    23821 ctttaatcag cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg
    23881 ccgctacgcc gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag
    23941 tgataatcag acacagaatg aatgcgaaga tcggccccaa tgggaagaaa cctgaacggt
    24001 acggcagatc gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc
    24061 taatggcaat ccccagccag gcgataaaac cggtcatccc ggaggtattc agcagccaca
    24121 ggtataccgt ctggttgcca aacatggagg tcaggaagca cagaccggca atcaccgtcg
    24181 tcgcatacag cgcattacgc ggcacgccac cacgcgacag tttagcgaaa atgcgcggcg
    24241 ctttaccgtc acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac
    24301 ccgctgacag caccgccgtc agaataactg cgttcatcac cgccgcagca gagagcagac
    24361 ccgcgtgctg gaacaccagg gtgaacggac taacgctgat gtctttaaca tcgttacgca
    24421 gcaggctcgg atcggtgtac ggaataatca ggctgataat caggatcgcg aacacataga
    24481 acaacaggat tcgccagaac acctgacgta ccgcgcgtgg aatgtttttc cccggatctt
    24541 cggactcgcc tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca
    24601 tagctacgcc gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt
    24661 tgctccagcc cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga
    24721 tgataaagac aataactgtc gtgactttga tcagtgagaa ccagtattcc gcttcaccaa
    24781 agccgcgaac agagatgtag ttcagcagga agataacgcc gaggaacaac gcactccaga
    24841 tccagcccgg tgtatccggg aaccaccagc tcatgaccag ctgagctgca accaggtcaa
    24901 cggcaatagt caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagccct
    24961 cttcaacata gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca
    25021 gttcaccgag actggtcatc aggaagtaaa ccatcaggcc aatcaacata tacgagagca
    25081 atgccccgcc cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac
    25141 cgatggaacc gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc
    25201 ccggcgcttc tgtggtttta gtttcggaaa ccatacaaaa atgctatcca tctaaaaaat
    25261 gaggcgcgat tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac
    25321 accttcatga tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca
    25381 tgctgaaggc gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcaactt
    25441 aacgcggcac atttgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt
    25501 gtttttgccg atgatgtata cgccacaacg tacgcatcag gcgcggcaga gggaccgcaa
    25561 cttcacttaa tgtgcctgcc tgcaattgat cttcaatcac acgtcgcgac aggcaactaa
    25621 ttcccaaccc atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca
    25681 tctcaaactt cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac
    25741 cgcgttcacg caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacggg
    25801 ccaacggcga agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa
    25861 tgatttcagt gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca
    25921 ccgcttgcat cacgtcctgg ctattcccaa cgctaagttc aatcggcaac tgcggataat
    25981 catggcgata acgggcgata actgcaggca gaatgtagtt accgatggta ctactggcat
    26041 agatacgaat cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa
    26101 gcaatgccaa cgcacgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc
    26161 ccacgcgatc aaacagttgc acgccaagct gcccttccag gtcggtcaag gctgcgctca
    26221 ctgctgattg cgacaacgcc agcatcaccg acgcttgggt ggttgatcca cttttcaata
    26281 cttctgcaaa aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa
    26341 gattaattat aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc
    26401 cggttaaagg agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg
    26461 cattttattc cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt
    26521 tccattcccg cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg
    26581 atggttttag gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg
    26641 tttgccaagc aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc
    26701 tcgcaaattg ccgatgtcgg tatcagtggg atcatcattg acgtgctgac gctgtccagt
    26761 accttcctgc tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc
    26821 tggttgatcg gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagccg
    26881 gtagtgaaag cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg
    26941 accgtcgcga ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg
    27001 gaaactttcg gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca
    27061 ggtcatgcca tcagcccgga tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg
    27121 atgatgctgg ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg
    27181 aacagcggcg agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtagtt
    27241 gccatcttta actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg
    27301 gataccttcc tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg
    27361 ctgaaaaaag ctggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt
    27421 gttggtggtg gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct
    27481 tgctcctgtt aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg
    27541 catgaaatac attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg
    27601 cgccgccgaa atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg
    27661 tgccgcaccg ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca
    27721 ctacacatcg gcgcaaattc ttccccacga cagttatctg attaacctcg gacatccggt
    27781 cactgaagct ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca
    27841 gctggggctt tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga
    27901 ggattgcctt gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt
    27961 gacagcggtg atagaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca
    28021 tctcgcggcg attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac
    28081 ctgccatgct ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg agaaaacatt
    28141 cgcggatttt gcccgtactg tcggctttaa gtatctgcgc gggatgcacc ttaacgatgc
    28201 gaaaagcacc tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg
    28261 tcatgatgcg ttccgctgga tcatgcagga cgaccgtttc gacggcattc cgctgatcct
    28321 cgaaaccatc aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac
    28381 tgaaaaagcg gtagcctgaa gatgaataac cgggaaaagg agatccttgc aattttacgg
    28441 cgtaacccgc tgattcagca gaacgaaatt gcggacatgc tgcaaatcag ccgttcgcgc
    28501 gttgcggcgc atattatgga tttaatgcgc aaaggccgga ttaaaggcaa aggttacatt
    28561 ctcaccgagc aggaatactg cgtagtggtg gggacaatca atatggatat tcgcgggatg
    28621 gcggatatcc gttacccgca atcggcttct catcccggta caattcattg ctcagcgggc
    28681 ggcgtgggac gcaacatcgc ccacaatctg gcgctgttag gccgtgacgt ccatttgctt
    28741 tcagtgattg gcgatgactt ttatggcgaa atgctcctgg aagaaacgcg ccgcgccggc
    28801 gtgaatgtct ccggctgcgt tcgtttacat ggtcaaagca catcgacgta tctggcaatt
    28861 gccaatcgag acgatgaaac cgtgctggcg attaacgata cccatctgct ggaacagtta
    28921 tcgccgcaat tattgaacgg gtcgcgcgat ttacttcgtc atgcgggcgt ggtactggct
    28981 gattgcaacc tgacagccga ggcgctggaa tgggtcttta ccctcgctgg tgagatcccg
    29041 gtgtttgtcg ataccgtttc agaattcaaa gcgggcaaaa tcaaacactg gctggcgcat
    29101 attcacaccc tgaaacccac tttaccggag ctggaaattt tatggggaca ggcgatcacc
    29161 agcgatgctg accgtaatgc tgcagtgaat gcgttgcatc agcaaggtgt tcagcaactg
    29221 tttgtttatt tgcccgatga gtcagtttat tgcagcgaaa aggatggaga acaatttttg
    29281 ctgaccgcac cagcgcatac gacagtagac agttttggtg ctgacgatgg ttttatggcg
    29341 ggcctggtat atagctttct ggaaggaaac aatttccgtg acagcgcccg ttttgcgatg
    29401 gcctgcgcgg caatttcacg cgccagcggc agcttaaaca accctaccct gtctgccgat
    29461 aacgcgcttt cattagtgcc aatggtgtaa caatgttgcc ggattcgacg cttgaccgca
    29521 tcttatccgg ctcataaatc gcacactacg ctaaaccaat aaagaacccg gcaatggtgg
    29581 cactcatcag gttagaaagt gtcgccgccg ccagcgcgcg taaaccaagc tgggcgattt
    29641 ccggcgcacg gtgtggcgca accgcagaaa acgcccccac caccaccccg atagaaccaa
    29701 agttagcgaa accgcacaac gcgaaggaaa taatcgccac ggttttagca tccagagtgc
    29761 cagccgtttg cagatagggt gagaaattga gataagcgac aaattcattt attgccagtt
    29821 tctgtccaat caaactcccg gcaagattcg catcactcca gtcaacaccc attacccacg
    29881 ccagtggggc cagcagataa cctacaatgg actccagcga ggcatgttca aaaccaaacc
    29941 agccgccaac gccgccgata ataccgttaa ttaacgcaat gatggcgacg aatgccatca
    30001 ctactgtcgc cacacctgcg gcgattttca gcccggtcat tgcccctgtc gcggcggctt
    30061 caataatgct ttttggcggt gtttcggtga aagagaggtt attaaaagaa acctgcgaag
    30121 attccgtagc cgggcttagc aggcgggcaa acaagatccc cccggggatc gccattaacg
    30181 atgccgccag caaatattcc acaggcacgc ccagtgcggc ataaccaatc attgtcgaac
    30241 cagcaatcga ggccatgccg ctacaaatcg ctgtaaataa ttcattgcga ttcagccgat
    30301 cgataaaggg cttcacaatc gccggaattt cgttttgccc gaggaaaatg gtagtgaccg
    30361 cgacgaatga ctcaatcttg ctgatattta atgctttctg gaatataccg ccgagaatgc
    30421 gaattaaaat ccccatcaca ccgatgtagt agagaatact caccagtgca gtgacgaaga
    30481 taattgccgg taataccctg aaaccaaaga taaatcctgc gccatcaaat aaggtgtcca
    30541 ttttcggtcc gaccagagaa ccgaagataa atgcgctacc cgcgtcgctg tacgccatca
    30601 ctttatgcac accaaaagcg actttttcag cgacccaacg ccctggcggt aaccaaagca
    30661 taatgccgcc aatcacgacc tgtaacacta acgccgcgcc aacggtacgc aggctgatct
    30721 tcttcttgtt tactgacagt aaaaacgcaa tcgtcagcaa taccaccatt cccagaacac
    30781 ttctcatgac atccatagtg attatctctt catgtcatat tgggcgacgg ctttctgccc
    30841 atcagaaata gccaggcctg catcttgcga taccggcccg gcttgttatc aacacttaat
    30901 gggttttgat gtagccgcgc acgcactctt cgactaagcc ccagaaccag tcgctatcaa
    30961 tagtgatgcc gactttggta ttagcgggct tgccaagaac gcccagctcg tcgcagacgg
    31021 tacgcccata acaagggcca ctgttgacgt ccacttcgac gtacatctcc tgggttttaa
    31081 tgccatcagg gttaatcaga taaccgatgc aggtggcgtc gtgcaccggg ccgccagcca
    31141 ggccgtagtt ttcgaactgg gttttgagag tgaagttcat gatgtcgctg aacagctctc
    31201 cggcagggcc gcctgccctt tccattcgtg caatcacgtc cggggtgcaa acggtctggt
    31261 tggtgagatc gaggcccatc atcactaatg gaacgccaga ggtgaacacc acgcgtgcgg
    31321 cttccgggtc agcaaagatg ttgaactcgg cagatggcgt gaagttgcct gtaccgtaag
    31381 cgccgcccat tagcacaatt tcacggattt tgggcaggat cgcgggttgc atacgcattg
    31441 ccaccgcgat atttgaaagc ggaccaaccg gcaccagagt gatatcgcca tcgcttgcca
    31501 tcagggtatc aatgatgtat ttcaccgcat gagtgctttc tgcctggcgg gtcagcggct
    31561 cgaataccgg gccatccagt ccggtatcac cgtgaatatt atcggcaacg atttgttgcc
    31621 gcataatggg ttgtggcatc cccgcataaa ccggaacatt aatctccagt ttctggcaaa
    31681 cattcaggcc attaattaat gttttatcaa gcgtctgatt acccgctaca atagtgatgc
    31741 ctaataaatc tattgccgga tgtttcgccg ccatcattat agcaatagca tcatcatgac
    31801 ccggatcaca atccagaata atttttctct tttccattgt ttatttcctc tgtttccagt
    31861 tgcgttattt tttctacagc aaagaaattt cgatcgggcc gatgattgaa tcttaacaac
    31921 agcgtacgta tgctaaatat gagaaatctc atagcggata aacatcgtga aagaaatcca
    31981 caataatgat cttaagcagc aattgatgag tgaatccgcg tttaaggatt gctttttaac
    32041 ggatgtttca gccgatacgc ggctgtttca ttttttagcg cgtgactaca ttgtgcagga
    32101 agggcaacag ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc tttacgccac
    32161 gctagctaat ggtcgcgtgt cgctgatcga tttctttgcc gccccctgtt ttattggtga
    32221 gattgagtta atcgataaag accatgaacc gcgtgcggta caggctattg aagagtgttg
    32281 gtgccttgcg ctccctatga aacattaccg tccgctgtta ttaaacgaca ccctattttt
    32341 acgaaaactt tgcgtcacct taagtcataa aaattatcgt aatattgttt ctttaactca
    32401 gaatcaatca tttccgttag tgaatcgcct ggcagcattt atattactct cacaagaagg
    32461 tgatctttat cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt cttatcgaca
    32521 tcttttatat gttctcgcgc aattcattca cgacggttta ttaattaaaa gcaagaaagg
    32581 atatctaatt aaaaacagaa agcagttgtc agggctggca ctggagatgg acccggagaa
    32641 taaattctcc gggatgatgc agtaaaaatt atttgcaata gcgcgattgc cggatgcaac
    32701 gcttgatgcg ttttatccgg tctacaaatt gagcattacg ccagaccaat aaagaacccg
    32761 gcaatggtcg cgctcatcag gttggaaagc gttgctgctg ccagcgcccg taaaccaagc
    32821 tgagcgattt ccggtgcgcg ttttggcgaa atagccgaaa atgccccaac gacaacaccg
    32881 atagaaccaa agttagcaaa accacaaagc gcaaaggaga taatcgcaat ggttttcact
    32941 tccagcgtgc cgcccgtttg caggtatggg gagaaactca ggtaagcgac gaattcgtta
    33001 atcgccagtt tctgcccaat caggctaccc gcaaggttgg catcactcca gtccacaccc
    33061 atgatccacg ccagcggtgc cagcacatag ccaaaaatac tttccagaga agcattggcg
    33121 aagccaaacc agccgccaat tccgccgata atgccgttga tcagcgcaat aattgccaca
    33181 aatgccatta ccaccgtcgc cacaccagcg gcgattttca gcccggtcat cgcaccgctc
    33241 gccgctgctt cgataaagct ttttggcggc gtttcgctga acgacagatt ttcaaacgtg
    33301 acctgcgaaa gctcggttgc tggactaaga atacgtgcaa acaaaatacc gccggggatc
    33361 gccatcagcg atgccgccaa caggtagtca attggtacgc ccattccggc ataaccaatc
    33421 atcatcgaac cagcaatgga cgccatcccg ctacaaatag ccgtaaacaa ctcgtttcga
    33481 ttcatgcgat cgataaacgg tttaacgatc gccgggatct cattttgccc gaggaaaata
    33541 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg gaaaatgctg
    33601 ccaaggatgc gaatcagcag ccccatcacg ccaatgtagt acagcagact aatgagcgca
    33661 gtgacgaaga taatcgccgg aagtacgcga aaggcgaaga taaaacccgc accgtcaaag
    33721 aggacatcca ttttcggccc gaccagcgaa ccaaaaatga aggcgctacc agcatcactg
    33781 taagacatca ctttatgaac gcctaatgcc gcctgttcta cagcccactt tcccggcgga
    33841 aaatagagca tgatgccacc aagagcaatt tgcagcagta gtgcggctgc aaccgtacgc
    33901 aaactgatgc tctttttatt cactgacaac agaaatgcta ttgccagtaa caccaccatc
    33961 cccacaacac ttctcattat gtccataatg attttccctt catgccagta aacccggcgt
    34021 cagcgccagg tttcggtatg tttgatgagt acgggcgacg gctttctgcc cgtcagaaat
    34081 taacccgcga gacgctgata ttctttggca atttcgctcg ccagaatggc gttgttgaac
    34141 acaagctgga tgttggattt caggctgtca ccgccggtca gctcagcaac gcgcgccagc
    34201 aggaatggcg tactttcttt accaataaca ccctgcgctt cagcttcagc taccgcctga
    34261 tcgatcaccg cattgatagt gtgttctggc atcgcaaact gttccgggat cgggttcgct
    34321 accacgaggc caccgttcag cccgctttgc catttcaccg ccattgcacg ggcaatttca
    34381 ctggcgctgt cgagacgaat gctgacgtca aacgggctgg tacggcagaa aaacgcaggc
    34441 agcgctttag tctgatagcc aattaacggc acaccgaagg tttctaaata ctcagtggtt
    34501 aatcctaaat cgagaataga tttcgccccg gcacaaacaa cggtgacatt agtatttgcc
    34561 agttcttgca aatcggcaga aatatcgaag gtatgttccg cccctcgatg aacaccacca
    34621 attccgccgg tggcaaatac tttaattccg gcaagcgccg caataatcat cgttgaagcc
    34681 acggtggttg caccattttt tcctgcggca acgacaaaag gtaaatcgcg acgactaact
    34741 ttagtcacgt tatgcccttc acgacccagt aattcaattt cttctttgct taaacccact
    34801 ttcatcacgc cgccaataat ggcgatagtg gcaggtactg cgccctgttt acgaatagtt
    34861 tcttctactt caattgcggt ctgggcattt tgtgggaacg gcatcccgtg agaaataatg
    34921 gtcgattcca gcgccacaac cggttttttg ttttttaaag cgtcctgcac ttccggggaa
    34981 atttgtaata attcagggga aatttttaat tcagacattc tgcgttctcc actaacgata
    35041 taacgttggc aatcgataaa tcggggttat tggtgtattc acaggagagc gccattgacg
    35101 aacatccctg tgcgaaacga acagattcgg caaacggcat tccgtctacc caacacgaag
    35161 caagtcccgc catcatggca tcgcccgctc cggtaacatt aataacattg gttttgatcg
    35221 gcgcagacca gccactttca ccgctgatat cgctgtaata aacgccgtcg ccgcccatgc
    35281 tcaataccag tcggttcagg ccatgttgat ggaaccaggc agcaactttt gccacatctt
    35341 cacgccctga cagcgcaatc ccactcaggg tttccgcttc aaggcggttt ggcttgagag
    35401 tatggatttg acttaatcgc tcgcgtactt tgacgcattt ccacgcggaa accggatcga
    35461 caaatacggg tacgttggca gcattatcca gaatccatgc cagtgcctct tcactgatat
    35521 tacagtccgc gacaatgacc tttgccctct gaataaattc acggtgctgt gcgagatatt
    35581 cagctgtaat agcgttgcta atattcatgt catttatagc aaccagcatt tcaccggtat
    35641 tatcgagtaa tgataaatag ctcgacgtat tttctcccgg cacaatcagg catttatcga
    35701 cataaacgcc agattgattg gtttgcgtta gcagcgattg gccataaaaa tcactgccta
    35761 cggcgctcag tagccaggct ttattaccca gcaacgccag gttttgtgca atattgcgcc
    35821 ctaccccacc aggcgtaaat tttattttac ctggatttga atccgcataa tttaatgatt
    35881 catgtgaata tccggcgaca tcaatattcg ccgagcctat aattacgaca taatcctttt
    35941 cgcgcatagc acgtcctctg gtaaaaaatt accaaccctg tcaatccaca aggatataga
    36001 tactgcaaag acggtttaaa catgtgttca tatttaaaca tgtgctcata gtatgcatgt
    36061 tttacgtaaa gtaaattgcc gccagagagg caaagcgggc agatttgagc aggctcacaa
    36121 aatgcgatta aatgcctgaa ttacaaaagc gaggaatttg tgacgggatg cacagaacat
    36181 gaacactaaa aaagggcaga aaagaatctg cccttgagga aataacctta gtgtttgatg
    36241 aacgtttcag gtttctactg aaagataaaa ccatttaaat tcataaaatt acattcacaa
    36301 accggtcatt ctctttgcgc tcatccaatg atacggggca gtaaatttct gccctgtaac
    36361 acacctttta ttacgcggct ttcgctactg cgtccacttc cggacgtttc aggaaggcat
    36421 aggccaaacc cgccaccagc gtaccggcaa taattgctac caggtaaccc agtaccggcg
    36481 taatagcgcc agggatcagc agaacaaaca gaccaccgtg cggtgccatc agtttcgcac
    36541 caatcgccat tgagattgcg ccagtcagcg ccccaccaac gatacaacac ggcagcacac
    36601 gcatcggatc acgagcagca aaaggaattg caccttccga aataaagcac agaccaagaa
    36661 ccagagcggc tttgccacct tcctgctgcg ctttgtcaaa tttgcgacgc gccaccattg
    36721 ttgccagacc cattgccagc ggtggcacca tacctgccgc cataatcgcc gccatcgggc
    36781 cataggtttg agtactcagc agacccacac cgaatgcgta cgctgcttta tttaccggac
    36841 cgcccatgtc agtacacatc atgccaccga ggatcgcccc cagcagaacc gcattcgcag
    36901 tccccatggt ctgcagccag tgagtcaacc cttcgagaat gccagcaacc ggtttaccga
    36961 tcaggtagat catcgccaga ccgaccacca gactggaaat tagcggaatg atcaggatcg
    37021 gtttcagcgc ctccatactc tgtggcagtt tcagttgcgt actgattaac ttcgcaatgt
    37081 aaccagccag gaagcccgca ataataccgc caatgaagcc agaaccggtg ctgaccgcca
    37141 gcataccgcc aatcagaccc ggcgtgagac ccggacgatc ggcaatggag aaggcaatat
    37201 aacctgccag taccggcacc atcagcgcaa aggctgaacc accaccaatc tgcatcagcg
    37261 ccgctgccaa cgtacccggc tctttaaacg cttcgatacc aaaagcaaaa gaaagcgcga
    37321 tacacagacc acctgcaacg accatcggca gcatgtaaga gacgcccgtc agcaagtgac
    37381 ggtatgcgcc tgcactctcg ttcttacctt cagaggtcgc cgtttgagct ttgcccgccg
    37441 gttcatacgg cgttgcttca gcaaccgctt tatccagttc ctgcgcggtt ttcttcagcg
    37501 ccagaccggt agaggtgcga tacatcggtt taccagcaaa tttcgccaga tccacttcga
    37561 tatctgccgc cacaatcacc agatccgctg cggctacttc ttcgggagtg attgcattac
    37621 ccgcgccaac agaaccacgg gtttcaactt tcacccacca gccacgtttt ttcgcttcgg
    37681 tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc agtcgggcaa gcagtcaccg
    37741 caactacgcg tttcggaccg ctggcggcaa ccggtgctgt cgcagcgacc ggcgcagtgt
    37801 aaggtttcgc atggcctttg gcttcactca ggaacagctc agggtgcgca actgcccggg
    37861 aaatatcgcc cagccagaca tttttaccgt tcagcgcgct gtcattcggg atggaatcac
    37921 cgagaacaat cgccatttct gcgtcgttcg gattgtcgat gatttccagt tttgcttttc
    37981 gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg tgcctgaccg agattagcgt
    38041 caataatcag cagcgttttc attatgcctc tcctgctgtc agttaaaagg ttgtaagtcg
    38101 acgcgcgcca tcattgcggc caactgcgga cgatcggtaa tacccacatt gctttgactt
    38161 accgccaggg ctgcaacagc agtcgccaga cgcagtgtgt gttcactgga ttcacgcatt
    38221 agcaagccat aaatcaggcc accaaccata gaatcccctg cgccaacggt gcttacgaca
    38281 tcgactgacg gtggtttggc gatccattcg ccggaggcat taacccaaag cgcgccttcg
    38341 gcacccagtg aaataacgac atgcgcaata ccttgttcac gcagcgcatg cgcagcttca
    38401 atcacatctt tcatttcagg cagtttacgg cctgcccaga tttccagctc gcggcggtta
    38461 ggtttcacca gccacggtgc cgctttcaaa cctgctacta acgcttcacg gctactatca
    38521 aagataatgc aagggcactg actacgcagg cgagtcatcc agtcggtgaa cgcttccggg
    38581 ctgacgcctg acggtaagct tccgctgaca cagaccatat cgaactgacc gagccagctc
    38641 agagaatcag tcacaaagcg ttcccagtcg gcgggggtga cttcaaaacc cgagaagttg
    38701 aagtcggtca cttcgccgtc tttttccgtc agcttaacgt taattcgggt gcgcccctgt
    38761 acaacctgga aacggttggc aatgcccagc tcgctgaaca gttgctgaaa accatcctga
    38821 ttgtctttac cgaggaagcc gccaacggtg acatcaattc ccaggtcttt taatactttg
    38881 gccacgttga tgcctttacc cgccgcatgc agaccggtgg ttttcaccag gttcacttcg
    38941 ccgcgttcaa tttccgggca gaaaccaaca aggtcataag ccggattaag ggtgatagta
    39001 gcaacacgtc tgctcattat gcgccctccc caagaccagc agcgatagcg tcgccgattg
    39061 ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa gcgtaggcga tgacctttct
    39121 taacgccaag tgccacaact ttcatcagac tacgtccgtt tgccggtttg ccggtgccat
    39181 caaggtttgt cacggtaata tcactgttaa attgtttaat ggtgttgacc agcatggtac
    39241 ctggacgagc atgcaggccg tgttcattgc gcaccacaaa ctccgcgctt aacacgtcgt
    39301 cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt tgccgcatcc gctttcagca
    39361 agcggtcagc tttattgtcg agcaacaaat cagcgagacg cttaagaacc gcaatgggct
    39421 gatcgtcatt catcgccaca ctcaccagca tggctgccgt ttcgccgtcc acatcaaaag
    39481 catttgccgc acggcttacc gcaattgcgc tacgcagatt gccttcggcg ctatcgctca
    39541 gccagatacc ctgtccgagg ttcagcggtt gttcattgat ggctttggtg acgaaagtgg
    39601 cgtcaactgc ccccgcctct ttcagacgcg cagcgttcag cgcctgaaga gtcagcagat
    39661 cgctggcgac gatatccagc gtcagcattt cgttgtcgag cttcagctgc tcactctgct
    39721 tttcgcccat cagcaatgcg cgaagttctt ctgctgttgt tgctgacttc agttgttcag
    39781 caacggaatc atcgctcagt acgtgggtca gctggcgtag caggcccaga tgctcatccg
    39841 agctggcagc aataccgatc gctacgtacg ctacctgacc gtcaccccag gtgacgcctt
    39901 ccgggaactg aaatacctga acgccggttt tcagcacctg atcgcgggtg tcggtagtgc
    39961 cgtgtggaat agcaatacca ttgccgagga acgttgaggt ttgctgttcg cgcgccagca
    40021 tgccattgac gtagccttct gctacattac cggcctgcac cagcgccgca gcgacctggc
    40081 gaatcgcctc ttctttgtct ccggcctttt cgcccggatg gatgtcctgt acggataact
    40141 ggaacatagt tctcctctct tgctgaattg aaacgattca gcttctatga gaaaaaaagc
    40201 gccaacctgg cttagggtta aagacaagat cgcgctgaaa cgtttcaaga aagcataata
    40261 cttctgtttc agcacgcaag gaaagccgta aagttatgag caaaagtttg atgtgctgca
    40321 caattttttg tcatttttcc taattgctga tgggaaaatc cgttatcagc aatttcattt
    40381 cagcagcgtc agcagccaat cagcaacagt gaaatggcat tttgattatt cgtggcgctt
    40441 ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa actccgcgtc ttcctcttcc
    40501 agtgatcgac cagcatgcat aactcccccg cagtctccag cgcgaaatcg tttgacctga
    40561 cctcgacggc gtttttaatc gttgcctttc tcaccggtat tgcgggcgct ctgcaaaccc
    40621 cgacactcag tatttttctt accgatgaag tacatgcccg tccggcgatg gtgggattct
    40681 tctttaccgg cagcgctgtc attggggttc tggtgagtca gtttctcgcc gggcgctctg
    40741 ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg cctgttaggc gtgctggcct
    40801 gcaccctttt tgcctggaat cgcaactact ttgttttgct attcgttggc gtctttctta
    40861 gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct tgcccgtgaa catgccgaca
    40921 aaaccggacg tgaggcggtg atgttcagct cttttttacg cgctcaggtt tcactggcat
    40981 gggtcattgg cccaccgctg gcttatgcct tagcgatggg tttcagcttt acggtaatgt
    41041 atctgagcgc agcggtagca tttattgttt gcggcgtgat ggtgtggctg tttttaccgt
    41101 cgatgcaaaa agagcttccg ctggcgaccg gcacggttga agcgccgcgc cgtaaccgtc
    41161 gcgatacgct gctgctgttt gtcatttgta cattgatgtg gggctcgaac agcctgtaca
    41221 tcatcaacat gccgctattt attatcaacg aactccatct tcccgagaaa ctggccggtg
    41281 tgatgatggg gaccgccgcc gggctggaaa tcccgaccat gttgattgcc ggatatttcg
    41341 ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc cgtgggtggc gtctgttttt
    41401 acgcaggaat gctgatggcg cattctcctg tcattctgtt gggcttgcag ctgctaaatg
    41461 ctatttttat tggcattctg ggcggtatcg ggatgctcta ttttcaggat ctgatgcccg
    41521 gtcaggcagg ttcagccacc acgctctata ccaacacgtc gcgcgtgggc tggatcatcg
    41581 cgggatcagt ggcgggcatc gtcgccgaga tctggaatta tcacgctgtg ttctggtttg
    41641 cgatggtgat gattatcgcc actctgtttt gcttactgcg gattaaagat gtttaaggcg
    41701 cggtcagcat ttccagatcg ataagccagg tcattgcctg ctgacgcgag ttaccgcaca
    41761 tttcagcgct ggcttgtaag cctgcacaga ctttcggccg cagtggcgag gtgaaaattt
    41821 tacagcgttg ctgttcatca agctgaatgc agggcgtatt ggcgggcttg ccatctggca
    41881 taccgggaat agggctggaa attgaagggg cagtgcaaca cgccccgcaa cccggacggc
    41941 attccatacg ttgttctctt aaggttaacc accggattga tgagcgcgca cagtaccacc
    42001 ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa aggctcgtcg cgagtacttt
    42061 gtcgcgatat ttttgacatt ttcgactaca ggaatttttc gatgccaaga gcgaacgaaa
    42121 ttaaaaaagg tatggtactg aattacaacg gcaaactgct gctggttaag gacattgata
    42181 ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa aatgcgtttt tctgatgtcc
    42241 gtaccgggct gaaagtagaa gagcgtttca aaggagatga tatcgttgac accgtgacgc
    42301 tgacccgccg ttacgttgac ttctcctatg tcgatggcaa cgaatatgtc tttatggata
    42361 aagaagacta taccccgtat accttcacca aagatcagat tgaagaagag ttgctgttta
    42421 tgccagaggg cggcatgccg gacatgcagg tgctgacctg ggacggtcaa ctgctggcgc
    42481 ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac cgcaccaggc atcaaagggg
    42541 catctgccag cgcccgtaac aaaccggcga cattgagcac tggtctggtg attcaggtac
    42601 cagaatactt aagcccgggc gaaaaaattc gtatccatat cgaagaacgc cgttatatgg
    42661 ggcgtgctga ctaacttcag ccgcatgcag aaaagggata gctcaggctg tccctttttt
    42721 aatttattat accaatcttc tattttgcgc ttcctgaaca ccttacgccc tcaattttca
    42781 ctcgttgatt gatcgccctc acacttcatc gcattaacaa tccagaccaa tttcaattgc
    42841 tgtcatataa ctttacactg tcattgttaa ttaatcgtta ctaagacatg actcctatga
    42901 atacaatcgc ctccgttacg ctcccgcatc atgtacacgc tccacgctac gatcgccagc
    42961 agttgcaatc acgtatcgtt cattttggct ttggagcctt tcaccgcgca catcaggcgt
    43021 tactgaccga tcgtgtgctg aatgcccagg gcggcgactg ggggatctgt gaaatcagct
    43081 tgttcagcgg tgatcaactg atgagccagc tccgcgcaca gaaccattta tataccgtgc
    43141 tggagaaagg ggcggacggc aatcaggcga taattgtcgg tgccgttcac gaatgcctga
    43201 atgcaaaact ggattcctta gcggcaatta ttgagaaatt ttgcgagcca caggtggcaa
    43261 ttgtttccct gacgattacc gaaaaaggct attgtattga cccggccacc ggtgcactcg
    43321 acaccagcaa tccgcggatt attcacgatc tacaaacccc tgaagaacct cactccgcac
    43381 cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg cggccttaca ccgtttaccg
    43441 tgctctcctg cgacaatatt cccgacaatg gccatgtggt gaaaaacgcg gtgctgggaa
    43501 tggcagaaaa acgttcgcca gaactcgccg ggtggataaa agaacacgtc agttttccgg
    43561 gaaccatggt cgaccgcatt gttccggctg caaccgacga atcactggca gaaatcagcc
    43621 agcatctggg ggtaaatgat ccctgcgcga ttagctgcga accgtttatc cagtgggtgg
    43681 tggaagataa cttcgtcgct gggcgtcctg cctgggaagt cgcaggtgta caaatggtga
    43741 atgatgtcct gccatgggaa gagatgaaac tgcggatgct taatggcagc cactcttttc
    43801 tcgcttatct gggttacctc tcaggattcg cccatatcag tgattgcatg caggatcgcg
    43861 catttcgcca tgccgccaga acattaatgc tggatgagca agcgccgaca ctgcggatta
    43921 aagatgtcga tttaacacaa tatgcggata agttaattgc acgttttgct aatccggcgc
    43981 tgaaacataa gacctggcaa atcgcgatgg atggcagcca gaaattaccg caacgcatgc
    44041 tggcaggtat tcgcatacat ctggggcgcg aaacggactg gtcgttgctg gcattaggcg
    44101 ttgcaggctg gatgcgttac gtcagcggcg ttgatgatgc cggaaatgcc attgatgttc
    44161 gcgatccgct tagcgataaa attcgcgatc ttgttgcggg cagcatcagt gagcaacgcg
    44221 tcaccgccct gctttccctg cgtgaagttt tcggtgatga tctgccagat aacccgcatt
    44281 ttgtgcaggc catcgaacaa gcccggcaac aaatcgcaca attcggcgcg catcaggcgc
    44341 tattaaacac cctcaaaatt taacgatttc tgcggttaaa gcggatgaag ctcgccttcg
    44401 tccgctctcc ccttctcttt tctgccgttt ttagccagga ttaacgctca gttaacttac
    44461 cagagtattg cggagctgtt gtgaccagga ccaacctcat caccggtttt ctcggcagcg
    44521 ggaaaaccac gtcgattctt catctgttag cccataaaga tcccaacgaa aaatgggcgg
    44581 tactggttaa tgaatttggg gaagtcggaa ttgatggtgc tttgctcgcc gatagcggcg
    44641 cactgctgaa agagatcccc ggcggctgca tgtgctgcgt taatggttta cccatgcagg
    44701 tagggttgaa taccttactg cgtcagggaa aaccagaccg cttgttgata gagccgactg
    44761 ggctgggcca tccgaaacag atcctcgatc tgttaaccgc accagtctat gaaccgtgga
    44821 tagatctgcg cgccacgttg tgcattctcg atccacgcct gctgctggac gaaaaaagcg
    44881 ccagcaatga aaacttccgt gaccagctgg ctgccgcaga catcattgtc gccaataaat
    44941 ccgaccgtgc gacacctgaa agcgagcaag cgctacagcg ctggtggcag caaaatggtg
    45001 gcgatcggca attaattcac agtgagcatg ggaaagttgc cggtcatctt ctggatttgc
    45061 cgcgtcacaa tttagccgag ttgcccgcca gcgccgcgca ttctcatcag catgtcgtga
    45121 aaaaagggtt agcagcgtta agcctgccag agcatcaacg ctggcgtcgc agtctgaaca
    45181 gtggacaagg acatcaggcc tgcggctgga tattcgacgc tgatacggta ttcgacacca
    45241 ttggcattct ggaatgggcg cgacttgcac cggtggaacg cgtcaaaggc gtgctgcgta
    45301 ttcccgaagg gctggtgcga atcaaccgtc agggcgatga cctgcacatt gaaacgcaaa
    45361 acgttgcgcc accggacagc cgtattgagc tgatttccag cagcgaagct gactggaatg
    45421 ccttgcagag cgcgctgttg aagcttcgtt tagcgactac cgcgtaaggt tgcctgcgtt
    45481 tttcagtaag ataattagag aaaatatgat taaaaatttg ccgcaaatag tgttgttgaa
    45541 tattgtcggc ctcgcgctgt ttctttcctg gtatattccc gttaatcatg gattctggtt
    45601 gccgattgat gcggatattt tttatttctt taatcagaaa ctggtcgaaa gtaaggcctt
    45661 tttgtggctg gttgcattga ccaacaatcg cgctttcgac ggttgttcac tgctggcaat
    45721 gggaatgttg atgctgagtt tctggctgaa agaaaacgcc ccaggcagac gacgtatcgt
    45781 gattattggt ctggtcatgc tattaactgc agtggtatta aaccagctgg gtcaggcatt
    45841 aattcctgta aaacgggcca gcccaacatt gacttttacc gatattaacc gcgtcagcga
    45901 actgctctct gtccccacga aagatgcctc acgagatagc tttcccggcg atcacggcat
    45961 gatgctgctt attttttcgg cattcatgtg gcgttatttc ggcaaagttg caggtcttat
    46021 cgcccttatt atttttgtgg tttttgcgtt tcccagagta atgattggcg cacactggtt
    46081 tactgacatc attgtcggtt cgatgaccgt gatattgatc ggtttgccct gggtgttgct
    46141 gacgccatta agtgatcgat taatcacctt ttttgacaaa tcactaccgg gaaaaaacaa
    46201 acatttccaa aacaaataac tcacagtact taacatcatc aggtttattt ttatagtgag
    46261 gataatcctg atgatgcgca ccgtgctttc atccatcgaa agcaaaaatc attctctaag
    46321 taaatgaatg gattgcatgc atttcactca atcgtacttt aattgaccaa tcccgcttat
    46381 taactttctg tatcactttt tcttataaaa aatcatgtaa aaccgctcgc caagaccgca
    46441 ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag attatcctca cagcatataa
    46501 ttttgtgcgt tagtccacag atttggcctt aaggaattgt ttcaacatgc cctggtaatt
    46561 agtctcgtgt cgcttggcat tttttttata acgatatttg tcgttaagga cttcaaggga
    46621 aaacaaacaa catggtcaaa tctcaaccga ttttgagata tatcttgcgc gggatccccg
    46681 cgattgcagt agcggttctg ctttctgcat gtagtgcaaa taacaccgca aagaatatgc
    46741 atcctgagac acgtgcagtg ggtagtgaaa catcatcact gcaagcttct caggatgaat
    46801 ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat tatggatcag tatgctgact
    46861 ggaaaggcgt acgttatcgt ctgggcggca gcactaaaaa aggtatcgat tgttctggtt
    46921 tcgtacagcg tacattccgt gagcaatttg gcttagaact tccgcgttcg acttacgaac
    46981 agcaggaaat gggtaaatct gtttcccgca gtaatttgcg tacgggtgat ttagttctgt
    47041 tccgtgccgg ttcaacggga cgccatgtcg gtatttatat cggcaacaat cagtttgtcc
    47101 atgcttccac cagcagtggt gttattattt ccagcatgaa tgaaccgtac tggaagaagc
    47161 gttacaacga agcacgccgg gttctcagcc gcagctaata aaccgtttgg atgcaatccc
    47221 ttggctatcc tgacgagtta actgaaagca ctgtttaggc agtgcttttt tgttttcatt
    47281 catcagagaa aatgatgttt ccgcgtcttg atccaggcta tagtccggtc attgttatct
    47341 tttaaatgtt gtcgtaattt caggaaatta acggaatcat gttcatacgc gctcccaatt
    47401 ctggacgtaa gctcctgctt acctgcattg ttgcaggcgt aatgattgcg atactggtga
    47461 gctgccttca gtttttagtg gcctggcata agcacgaagt caaatacgac acactgatta
    47521 ccgacgtaca aaagtatctc gatacctatt ttgccgacct gaaatccact actgaccggc
    47581 tccagccgct gaccttagat acatgcaagc aagctaaccc cgaactgacc gcccgcgcgg
    47641 cgtttagcat gaatgtccga acgtttgtgc tggtgaaaga taaaaaaaca ttctgttcat
    47701 ctgcgaccgg tgagatggac attccactca atgaattgat tcctgcgctc gacattaata
    47761 aaaatgtcga tatggcgatc ttacccggca cgccgatggt gccgaacaaa cccgcaatcg
    47821 tcatctggta tcgcaaccct ttgctgaaaa atagcggcgt ctttgccgct ctgaatctca
    47881 acctgacgcc ttctctcttt tatagctcac ggcaggaaga ttacgatggc gtcgccctca
    47941 ttattggcaa tactgcgcta tctacctttt cttcacgttt gatgaacgtt aacgaattaa
    48001 ccgacatgcc agtccgtgaa actaaaattg cgggcattcc tctgaccgtt cggctttatg
    48061 cagatgactg gacatggaac gatgtgtggt acgcattttt actgggcggc atgagtggaa
    48121 ctgtcgttgg cctgctctgc tattacctga tgagcgtacg tatgcgcccc ggcagagaaa
    48181 tcatgaccgc catcaagcgc gaacaatttt acgtggcgta tcaaccggtg gtggatacac
    48241 aagctttgcg agtaacgggc ctggaagtac tgctacgctg gcggcatcct gtcgcgggag
    48301 aaattccccc ggatgccttc attaactttg ccgaatcgca aaagatgatt gtgccgctga
    48361 ctcagcacct gtttgagtta attgcccgcg atgccgcaga attagaaaaa gtgctgccgg
    48421 taggcgtcaa atttggcatt aacattgcgc cggaccatct gcacagcgaa agctttaaag
    48481 caaatatcca gaaactgctc acttccctac ccgcacacca tttccagatt gtgctggaaa
    48541 ttaccgagcg cgatatgctg aaagagcaag aagccacaca actcttcgcc tggctgcact
    48601 cggtcggcgt agaaattgct attgatgact tcggcaccgg gcacagcgcg cttatctatc
    48661 ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg atttatcaac gccatcggta
    48721 cggaaacgat cacttcaccc gtacttgacg cggtgctgac gctggcgaaa cgcctcaata
    48781 tgctgacggt tgctgagggg gtcgaaacgc cggaacaggc gcgatggcta agcgaacgcg
    48841 gcgttaattt catgcaaggc tactggatta gtcgcccgtt accgctggac gattttgtcc
    48901 gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc cttattattc
    48961 atagtgaaag catgccggat tgcggctaat gatgagtaaa aggaaatccg ttgcagatga
    49021 ttgtgcgcat actgctgctg tttatcgctc tgttcacctt tggtgcgcag gcgcaggcta
    49081 tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg gtacgcgttt aatttcaacc
    49141 attttgatta tgtgaacccc gccgcgccaa aaggtgggca gataacgttg tcagccctcg
    49201 gtaccttcga taatttcaac cgctatgcac tgcgcggcaa cccgggcgca cgcaccgagc
    49261 agctgtacga cacgctattt acgacttccg atgacgaacc aggcagttat tacccgctga
    49321 ttgctgaaag cgcacgctat gctgacgatt attcctgggt ggaggtcgct attaatcctc
    49381 gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga tgtagagttt acttttcaaa
    49441 aatttatgac tgaaggcgtg ccgcaatttc gtctggtcta caaaggcacc accgtcaaag
    49501 ccattgcgcc gttaaccgtg cgcattgagt tagctaaacc cggcaaagaa gatatgttga
    49561 gtctgttttc gctgccggta tttccagaaa agtactggaa ggatcacaaa cttagcgatc
    49621 cgctcgccac gcctccgctt gccagtggtc cgtaccgaat tacgtcctgg aaaatggggc
    49681 aaaatattgt ctattcccgc gtgaaagatt actgggcagc aaacttaccg gtaaaccgtg
    49741 gacgctggaa tttcgacacc attcgctacg attattacct cgatgataat gtcgcctttg
    49801 aagcgtttaa agcaggtgcc tttgatttgc gtatggaaaa cgacgccaaa aactgggcca
    49861 cacgctatag cggtaaaaat ttcgataaaa aatacatcat caaagatgag caaaagaacg
    49921 aatcagccca ggatacgcgc tggctggcgt ttaatatcca acgtccggta ttcagcgatc
    49981 gccgggtccg ggaagcaatc accctcgcct ttgactttga atggatgaac aaggcgttgt
    50041 tttacaatgc ctggagtcgc acgaacagtt attttcagaa taccgaatac gcggccagaa
    50101 attaccccga cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat ttaccgccag
    50161 aagtcttcac acaaatctac cagccgccgg tatcgaaagg cgatggctac gatcgtgaca
    50221 acctgttaaa agccgacaaa cttctcaacg aagcgggctg ggtgctgaaa ggtcagcaac
    50281 gcgttaatgc cactacgggt cagccgctca gctttgaatt attgcttcct gcaagcagca
    50341 atagtcagtg ggtattgccg ttccagcaca gcctgcaacg gctgggtatc aacatggaca
    50401 ttcgcaaggt ggatcactcg caaatcacca accgcatgcg cagtcgcgac tatgacatga
    50461 tgccgcgcct atggcgggcg atgccgtggc ccagttccga tttacagatt tcctggtcat
    50521 cggaatatat caattccact tataatgccc ccggcgtgca aagcccggtt atcgactcgc
    50581 tgatcaacca aattattgcc gcgcagggaa ataaagaaaa attactgccg ttagggcgag
    50641 cactggatcg cgtattaacg tggaattatt acatgctgcc aatgtggtac atggcggaag
    50701 accgtctcgc ctggtgggat aaattctccc aacccgctgt gcgccctgtt tacagtctgg
    50761 gtatcgatac ctggtggtat gatgttaata aagcggccaa actgccgtca gccagacaac
    50821 agggagagta gatgggcgct tatctgattc gccgtctgtt gctggtgatc ccaacgttat
    50881 gggcgattat cactatcaac tttttcatcg tgcaaattgc gcctggcggt ccggtcgacc
    50941 aggccatcgc cgccattgag tttggtaatg ccggagtatt acccggcgca ggcggtgaag
    51001 gcgttcgcgc cagccatgcg caaacgggcg tcggtaatat cagcgacagt aattaccgtg
    51061 gcggacgcgg attagatcca gaagtgatcg ccgagatcac tcatcgctac ggtttcgata
    51121 agccgatcca cgaacgttac ttcaaaatgc tctgggacta catccgcttt gattttggcg
    51181 atagcctgtt tcgcagcgcc tcggtgctga cgctgattaa agacagtctg ccggtttcca
    51241 tcaccctcgg attgtggagc acgctgatta tctatctggt gtcgattccg ttaggcattc
    51301 gcaaagctgt ttataatggg agccgctttg acgtctggag tagcgcattt atcatcatcg
    51361 gctacgccat tccggccttt ttgtttgcca tcctgctgat tgtcttcttc gcaggcggca
    51421 gctatttcga cctgttccct ctacgcggcc tggtttccgc taactttgat tcgctgccgt
    51481 ggtatcagaa aatcaccgat tatctgtggc atatcacgct gccggtgctg gcgacggtga
    51541 ttggtggctt tgcggcgctg actatgctga caaaaaactc attccttgat gaagtacgca
    51601 agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga aaaaaatatt ctctggaaac
    51661 atgtgttccg caacgccatg ctgctggtga ttgccggttt tccggcgacg tttatcagca
    51721 tgttttttac cggctcgctg ctgattgagg tgatgttttc actcaatggt cttggcttac
    51781 tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc ttgtatattt
    51841 tcaccctgat tggcctgctg ctgaatattg tcagtgatat cagctatacg ctggtcgatc
    51901 cgcgtataga ttttgaggga cgttaatgtc gcgactcaac cccgtcaatc aggcccgttg
    51961 ggcgcgtttt cgccataacc gtcgcggcta ctggtcgtta tggattttcc tcgttttgtt
    52021 tggtttgagt ttgtgttctg aacttatcgc caacgataaa ccgttgctgg tgcgttatga
    52081 cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa agcgattttg gcggcccgct
    52141 ggcaagtcag gctgattatc aggacccgtg gctgaaacaa cggctggaaa ataatggctg
    52201 ggtactgtgg gcaccgattc gctttggtgc taccagtatc aactttgcta ccgataagcc
    52261 cttcccttct cccccctccc ggcaaaactg gctgggaacg gatgccaacg gcggcgatgt
    52321 gctggcacgt attctctatg gcacgcggat ctcggttctg tttggcctga tgctgactct
    52381 ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa ggctattacg gcggtaaggt
    52441 cgatctctgg gggcagcgct ttattgaagt atggtcgggg atgccaacgc tgtttttgat
    52501 tattctgctt tccagcgttg tgcaacctaa cttctggtgg ctgctggcaa ttactgtctt
    52561 atttggctgg atgagtctgg tcggcgtggt gcgggcggag tttttacgta cccgcaattt
    52621 cgactacatc cgcgcggcac aggcgcttgg cgtcagcgat cgcagtatta ttctgcgtca
    52681 tatgttgccg aatgcaatgg tcgctaccct caccttttta ccgtttattt tatgtagttc
    52741 gatcaccacc ctgacctcac tcgatttcct cggcttcggt ctgccgctcg gttcaccgtc
    52801 actcggtgaa ctgctgttac aagggaaaaa taaccttcag gctccgtggc ttgggatcac
    52861 cgccttcttg tcggtggcga tattattgtc tttgctgatc tttattggtg aagccgtccg
    52921 cgacgcattt gatcctaata aggcggtgta gcatgacgca aactctgtta gcgattgaag
    52981 atttgtcggt gggttttcgc catcagcaaa ccgtacgtac agtagtcaat gatgtttcac
    53041 tacagattga ggctggcgaa acgctggcgc tggtgggtga gtcaggttca ggcaaaagcg
    53101 ttaccgcgct gtcaatttta cgcctgctcc cttccccgcc ggttgaatat ctctccggcg
    53161 atattcgttt tcatggcgaa tcgctgcttc atgccagcga ccaaacgttg cgcggtgtac
    53221 gcggtaataa gatcgccatg atttttcaag agccgatggt gtcgttaaat ccattgcata
    53281 ccctggaaaa acagctttat gaagtgcttt cactccaccg cgggatgcgt cgggaagcgg
    53341 ctcgtggcga aattcttaac tgccttgatc gcgttggtat ccgccaggcg gcaaaacggc
    53401 tgacagatta tccgcatcag ctctccggcg gcgaacgcca gcgggtgatg attgcgatgg
    53461 cgctgttaac gcgaccggaa ttattaattg ccgatgaacc gaccaccgca ctggacgtct
    53521 ctgtccaggc gcagatttta cagctgttgc gcgaactgca aggcgagttg aatatgggca
    53581 tgctgtttat tactcataac ctcagcattg tcagaaaact ggcccaccgc gtggcggtaa
    53641 tgcaaaacgg tcgctgtgtc gagcaaaatt acgccgctac gctatttgcc tcacccactc
    53701 atccttacac acaaaagcta ctcaacagtg aaccgtcagg cgatccagtg ccgttgccag
    53761 aacctgcctc aacgttgctg gatgttgaac agcttcaggt tgccttcccc attcgcaaag
    53821 ggattttgaa gcgcattgtg gatcataatg tggtggtgaa aaacatcagt tttacgctac
    53881 gagcgggtga aacactgggt ttagtgggcg agtccggttc cgggaaaagc acgacgggac
    53941 tggcgctgct gcgactgatt aattctcagg gcagcatcat ctttgacggt cagccactgc
    54001 aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg gtatttcagg
    54061 atccaaactc ctcgctcaac ccacgactca acgttttgca gattattgag gaaggcttac
    54121 gggttcacca gccgacgctt tctgccgcac aacgcgaaca acaagtgata gacgtgatgc
    54181 atgaagtggg attagatcct gaaacacgcc accgttatcc ggcggagttc tctggtggtc
    54241 agcgacaacg tattgcgatt gccagggcat taattcttaa gccctcgctg atcatacttg
    54301 atgaaccgac atcatcactc gacaaaacgg tacaggcgca aatattgacg ctattgaaat
    54361 cattgcaaca aaagcatcaa ctggcctatt tgtttatcag ccacgatttg cacgttgtcc
    54421 gcgcgttatg tcatcaggtt atcgtactgc gacaagggga agtagtggaa caaggaccgt
    54481 gcgcgcgcgt gtttgccaca ccgcagcagg agtatacgcg tcagctactg gcgttgagct
    54541 gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa ttgccacacc aaaatttttc
    54601 aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa acaggcacgg ctcaccttca
    54661 cactccagca ccgaacatgg aacttcgatg tcgcctaacg cctggcttag tttatacatc
    54721 accgaccatg cattatcacc atccgggcta agcaatttaa gcgccaccag gctgttgtca
    54781 ccgcacggtc cattttgcgg aatcggttca accttcgaac ctgcgaaacc agcagaccag
    54841 cgatagttct gcggcagtcg atggacgatt gagagttgta atgaagtcac ttattttccc
    54901 cggaagcaca tttacttcac aatttgttta catcaatttt aacacatcat caacaatccg
    54961 tctttataca gatcgtaaag attgatgcct cgcctgcgtg gcctctatgg ctctgattta
    55021 agtaatatct gcgggcgtgg tcacgtttgc ggggctatat gtacccgttt ctgcgatcta
    55081 actcaacctt tttaactaca atgatgtgac tttttacata aattgatttt acataaaata
    55141 aacatatatc gggggaatga tagatttgtg gttgacatgc atcaacaaag gaagcctttt
    55201 agcttcctcg ttgtgcaata gatcaccgtt ttttcggccg actggcgtac agacagaaga
    55261 gaatggagct ggttgcgcag aatgcaattg accaaatcat cggccatgca gagttaaagg
    55321 tcgcaagaga aagcaatgcg ccaacaattg cccctatgcc aaaacggaag gttcctgcca
    55381 gcgaagatgc cgttcccgcc atatggggaa actcatcaag aatgaccgcc atcgcattgg
    55441 atgacaccat cgacacgcag cccacaaacg ccgcaacgcc aaccaccagc gaccaaaatc
    55501 ccagacccag cagcgcactg atgaccatcc acgctgccat aataaattgt atccacaacc
    55561 ccgagcggaa catatttaac gcgccaatac ggcggacgaa gcggctatta aagatggtca
    55621 tcacgaacag gaaaacaatg tttagtgcaa agtaataacc aaagttttcc ggcgcgacgt
    55681 ggttaatttc aatgtagaca aacggtccgg cgcttaagaa cgagaacatc ccggcaaagc
    55741 tgaaaccact ggccagcata taactcagga cacgcttatg acggaacagc gccgcaaagt
    55801 taccaatagt ggtacgaatg tgaaaaggct ggcgacgctc cggtggtaag gtttctttga
    55861 tcagaaagaa gatcattgcc gaagccagaa tcgccgccac ggcgaggatc cagaagatgt
    55921 aatgccagct cagccacacc agcacccagc cgccaactat cggtgccatc agcggtgcaa
    55981 tggtcgtcac cagcatgaca aacgacatca tccgcgagaa ctcttctttc gggtagatat
    56041 cacgcatcaa agcgttgata accacgctgg ccgcagccgc cgccagcccg tggaagaaac
    56101 gcatcacaat cagctgatcg atggtttgtg ccaacgcaca cgccaccgcg gcggcggcaa
    56161 acaccagcgt accgccaaga accaccggct tacgcccgaa gctgtctgcc atcggcccgt
    56221 agattaactg ccccaacgca aagcccagaa tataagtact gagggtcatc tgcgtactgc
    56281 ccgccggtac gccaaactgc gctgaaatta ccggtagcgc gggcagatac atatcaatcg
    56341 acagcggcat caacatggcc agcaggccaa ggataaaaac aatagcaaac gacgaatgct
    56401 gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag ttagacgacg ctggcaattt
    56461 cttcttcagt taacggacga tattcaccgg gggctaaatc agcatccagc gtaatgccgc
    56521 caatacgttc acgatgcagc tcaaccacat ggttacccac ggcggcgaac atgcgtttca
    56581 cctgatgata acgcccttcg ctgatggtca gacgaacctg cgttggggta atcacttcaa
    56641 gcaccgctgg cttagtgaga tctttttcgt tatgcagctg cacgccttta gcaaattgct
    56701 ctgccgtatc gtcagctacg ggtgattcca gcgtcaccag ataggtcttc tcgcaatgat
    56761 ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc tgtcattagt accagaccgg
    56821 tggtatcgat atccagccgc cctgcggcgt gcagtttcca cgccactggt tcatctagaa
    56881 aatagagcac cgttgggtga tcagggtcgt ccgtggagca aacatagccc tgaggcttat
    56941 tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg gttgccatcg taagcgacat
    57001 catgttcagg aagcagtttg aacgctgcat tacggacgat ttcgccatcg acggtgacac
    57061 gattgccgcg gatttcacgc ccggcaatag cacggctaac gccgagttgc tgtgcgataa
    57121 atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaac ggaggtcggg cattgacccg
    57181 aaaatctgaa ctgttgtctg cccagtatag cggtctaata acttccctca agggaaaaag
    57241 aaccgtggca tactattagc agaataatct acctacgcga gaccatgatt tttacactcc
    57301 gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt cataaaaccc
    57361 ctgccgttat cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg gaacttgcgc
    57421 ggctggctcg tggtcgcgtg ctggtgctgg cacacgttaa agaactggtg gcgcaaaacc
    57481 atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgccggg ctaaagcgca
    57541 aagagagcca cggtaaagtg gtatttggca gcgtgcagtc ggtcgcccgt aatcttgatg
    57601 cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt ggtgacgatg
    57661 aagagagcca gtatcagcaa atcctcactc acctgacaaa agtgaatccc cacttacgcc
    57721 tgctggggct gactgccacg ccttttcgac tgggcaaagg ctggatctac cagttccatt
    57781 atcacggcat ggtacgcggc gatgagaaag cccttttccg tgactgcatt tatgaactgc
    57841 cgctgcgtta tatgattaaa cacggctatc tgacgccgcc agaacgactg gatatgccag
    57901 tagtgcaata cgatttcagc cgcttgcagg cacagagtaa cgggctattc agcgaagccg
    57961 atctcaaccg cgagctaaaa aaacaacaac gtattacccc gcacatcatc agccagatta
    58021 tggagtttgc tgcaacgcgc aaaggggtga tgattttcgc cgccaccgtt gaacacgcaa
    58081 aagagattgt gggattacta cccgccgaag atgctgcact gattactggc gacacccccg
    58141 gcgctgagcg cgatgtgtta attgaagatt ttaaagccca gcgttttcgc tatctggtca
    58201 acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc gccattctgc
    58261 gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg cgtctcgctc
    58321 ctggcaagac tgattgctta attcttgatt atgcgggtaa tcctcacgat ctctacgcgc
    58381 cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt ttctgccctg
    58441 cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca ttgattgaac
    58501 actttggtcg ccgctgtcag ggatggtttg aagatgacga cggtcatcgc gaacagtgtg
    58561 acttccgttt ccgttttaaa aattgcccgc aatgtaacgc agaaaatgat attgccgctc
    58621 gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta aaagcggcgc
    58681 tacgactgaa agatgcgctg gtattacgct gtagcggcat gtctttgcag catgggcacg
    58741 acgagaaagg cgaatggttg aaaatcacct attacgatga agacggcgcg gatgtgagtg
    58801 agcgtttccg tctgcaaacg cccgcccagc gaactgcctt cgagcagctt tttatccgcc
    58861 cgcatacgcg cacaccgggc atccctctgc gctggatcac cgccgccgat atcctcgccc
    58921 agcaagcctt attgcgacac ccggattttg tcgttgcccg catgaaaggt cagtactggc
    58981 aagtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac gaattacgcg
    59041 gttaatggta gttttgattg atgtataagg cgattgagta tagaatctcg cccgcttttg
    59101 catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat taatttaaga
    59161 gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa gggtgcgagc
    59221 cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa agaagcaccg
    59281 ctggctatcg agctggatca cgacaaagtc atgaacattc aagctaaagc tgaattctac
    59341 agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc tcaggacgta
    59401 cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc ttaattgctg
    59461 aataagttgt aaaaaacccc gctccggcgg ggttttttgt atctgcagat tatgcctgat
    59521 tacgatattg ctattttttg caggccagat aaggcgttca cgccgcatct ggcattcata
    59581 acacaaaacc gattaattgc cgccggacgt ccggcgctgc aattgatcgc gcaaattcgg
    59641 cggcgtgcct ttaatagtca gggtatcggt cgccggatcc cagaaaatcc gttcgcccaa
    59701 cagcatcgca tcaaaattaa tagtcaagcc accgccgcta ccggcaaact tcgtcaactg
    59761 acgcagtgtg ctacggtctg ccgggaagct ctcttccagt tcgtagcctt tttcagccgc
    59821 aaactccgtg aagctcactt cgctgacgcc tgccagctct ttcgacagcg attccagttc
    59881 gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct ggcgcacgtt
    59941 ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg cttgcaacag
    60001 cccacggttt tgcgctttcg cgttcaatcc ttcgctggcc ccgaggaaat ccataaagaa
    60061 atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg tcgactctgg
    60121 attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat tgatatcaag
    60181 ataatgggtt gggttgatgt cgagattttc gttaacgcgc atactgctca ggttgctcag
    60241 gactgccacc agcagatact ccaccgccag ataacggtag tggcaaaaca gcacgaatcc
    60301 accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgaccggttg ccgcgcggct
    60361 aaatgccaga aagtcctctt caccctgacg ctgtaagcgc agcgtctgcg ccagttcact
    60421 ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat gcagttctgc
    60481 taccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca ccagctcaag
    60541 gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat ccagactcat
    60601 gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc gacgcaataa
    60661 aagatggcgg tcattttaca gaaggcggcg taacgagaat gatttaacgc ataaaaaagc
    60721 agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact gatttcgatt
    60781 tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg cagagctgct
    60841 caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt taggcaacat
    60901 ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa ttgccaactc
    60961 ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag ggaaacagat
    61021 aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag atggtcagtt
    61081 gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc agccgttacc
    61141 tgtttatcgc cgactggccg accacgcttg ctggtcgcat ttattcctac gtaagcatta
    61201 tcggccattt cagcttcctg gtgttcgcca cctacttgct gattctcttc ccgctgacct
    61261 ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca acggcgggaa
    61321 tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat cttaatccca
    61381 tcgtctggca actggttatt aacccagacg aaaatgagat ggcgcgcgac tggcagctga
    61441 tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg tggagctggc
    61501 aaaagctgcg cagcctgacg cgtcgtcggc gcttcgcgcg tccgctggcc gcattcttat
    61561 ttatcgcctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac ttctatcgcc
    61621 ctatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg cgacgttttc
    61681 ttgagaagca tggcctgctt gatgcgcagg agtatcaacg ccgtcttatt gagcaaggta
    61741 atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc gatatgggca
    61801 ccgggcagaa tgtcttgttg attactgtcg atggcctgaa ctactcacgc ttcgagaagc
    61861 agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc catatgagct
    61921 ccggcaacac tacagacaac ggcatctttg gcctgttcta tggcatctcg ccgagctata
    61981 tggacggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt aatcagcaag
    62041 gctatcagct ggggttattc tcatcagatg gctttaccag cccgctgtat cgccaggcat
    62101 tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag accgccacgc
    62161 agtggatcaa ctggctggga cgctacgcac aagaagataa ccgctggttc tcgtgggtct
    62221 ctttcaatgg tactaacatt gacgacagca atcagcaggc atttgcacgg aaatatagcc
    62281 gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg cgtgattctg
    62341 gcaaactgga caatacggtg gtgattatca ctgccggtcg gggtattcca ctgagcgaag
    62401 aggaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg attcactggc
    62461 caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg atgacgacgc
    62521 tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa ggtcaggatt
    62581 tgttcaaccc tcaacgccgt cattactggg ttaccgcagc ggataacgat acgctggcaa
    62641 ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc acttacaact
    62701 tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg caagtactga
    62761 cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg aaatatctta
    62821 cttgcaatcg gtgtggaaaa cggtagtatt aacaaccacg agtcggcacg tagcgcagcc
    62881 tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc gtgccgacca
    62941 aaaatcccaa aaaaaccaac cgtaaaggtt ggttttttta tatctgcatt taattcgata
    63001 aacagaccgc gacacatcag ggcctgttta ttgtctgtta tcagaacgtc cagaccacac
    63061 ccgcctgggt attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat
    63121 gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccacgggttc
    63181 cagccacatc attattaaat ttgccatcgt cattaacttt cacctggtta gaatcggcgt
    63241 attcctgacg cacggcggct ttcagccagg gttccagcgt cgtaccgttt tgcaggtcca
    63301 tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg
    63361 cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca
    63421 gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctcg acatgagcac
    63481 ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga
    63541 tggtgttggc aaaacggtca actttcacca ctccatcaac ataggcaccg ttctgatgct
    63601 cccaaccggc ataagccccc agggtatagc tatcgacatt gcctttgccg ccgcgatcaa
    63661 aaccaatatc agaatgagag taaccaaaga acaagccgcg aattgtgctg ctttcttcac
    63721 gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc
    63781 ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc gccgtatcgt
    63841 aactaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata
    63901 ccagcggttg tgcggccgcc atattcagca catcagtggt tgaaggggta atttgcgcgc
    63961 gattctctgc cagactccag ctatggttac cattatccag caaggtatat tcatacgtac
    64021 cgatatcaac aacgcctccg gcattgccca acgtaaatgc agcatcaccg ccgcccgttg
    64081 ttaccagtgt aaggctatct cctgctgccg ggctggcacc ggtgtccgtc acgaatattt
    64141 tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc gctctgatga ttcgccatat
    64201 cggtacgcat ataaaaattg ccgtttcccg acagggtatt ggtggtcaac gtagtgaaat
    64261 tttccgcact tgccgtcaca gatgaacgag tcactgactc atccatcata atcacatttg
    64321 cgccagacat cgcaaggttg gctaagttaa ggcgggtgtt ttggttcgtc gtagtgatat
    64381 cggcaatgat ggtccatgcg ctattttcga gcgaaacatc cgctttgctg gtatccacgg
    64441 caccatgcgt tcttaaagaa gcgccctcag aaaccactac cttacccacc aacattgcgg
    64501 gtgcataatt tatttgcggc ttcataacct caagaatgcc atcatttcct ctgactgaca
    64561 cgttaaccat tgtgccagcc cagacgttag cgcggccatt gttgataacc atattttcag
    64621 agatggcttt agaggaatac gttatacttc cattctgatc tgatcgaccg agatcatata
    64681 ccgcatttgc ctgcactctc gtaccagtat cctttcccag cgattgcata gtggcatgct
    64741 catcgaggat agtgtcaatg gcctgcgtgt cctccataac aataagcccg gaaccatcat
    64801 ccagttcata attttttgac acaccatctt ttatactaaa ttggccttta ctgtttgtac
    64861 cactcacttc cagcgcattc gttgagacaa ttaattttcc gcctgctttt ttatcaacgc
    64921 cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatctaca gtagtattat
    64981 aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacagc atattgttag
    65041 caataccatt cttgatttcg aactgaccga gacggtttgt tccgaatacc tccatgaccc
    65101 gcgtgcttgc tttaatagca ccgcctgctt tctgatctac cgcaatcgct aatcccccct
    65161 ctttaacctc cagtaatcct cccttttcga tagcggcaag gacggtaatc ccatcttttt
    65221 caacgattaa gcgcccgcct ttaatcgtgg gatcattcgc tgcgccataa acctgtagca
    65281 caccattatt gagcgtagtc tggtttgccg cgccacccat ttgaacaatt tgataaccat
    65341 catttaatac tgagtattct gcggtgccat tcatttcaat gtactgatac ccgccgttaa
    65401 tttcagtatt actacttata ccaaattctt taatatgctg ttctccgcca ggattgattt
    65461 tcgtattatt ggcggtccca taaaccagct ggatatcttt ttccaggaca acaccatcaa
    65521 ctgtctcacc atactcaact gttgatgcca atgctatggg tgaatataac gctgtagcta
    65581 ccagaacaga tattgcggaa cgacaaagag aaacagaacc agattgatgc attgagcttt
    65641 catcctatga aattaattgc agttaaaagc attgggtaca gaaaataccc atagctccat
    65701 acccggagtc agtttttaaa aactgtttaa agaaatgcac aagtattgtg attgattttt
    65761 tagttgtttt tcttgatgag aagctgatgc aaaattccgt ctttataatg aaaatgatgc
    65821 caaagcgaac gacaaggttg tagttttcgc tacatgtcca tacataaaat ggggtaacat
    65881 tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat aatgattcta ataaaacctc
    65941 aggagactac tatgcctgaa gcaacgcctt ttcaggtgat gattgtggat gatcatccac
    66001 ttatgcgacg cggtgttcgt cagttactgg agcttgatcc tggctttgaa gtggtcgccg
    66061 aagcgggcga cggcgcgagt gctatcgatc tggcgaatag actggatatc gacgtgatct
    66121 tgctggatct caatatgaaa ggtatgagtg gcctggatac cctcaatgcc ttgcgcaggg
    66181 atggcgttac cgcgcaaatt attatcctga ccgtatcaga tgcctccagc gatgtctttg
    66241 cactgataga cgcaggcgca gacggttatc tgttgaaaga cagcgacccg gaagtgttgc
    66301 tggaagcgat tcgtgccgga gcgaaaggca gcaaagtctt tagcgaacgc gtcaatcagt
    66361 acttacatga acgtgaaatg tttggcgcgg aagaagatcc cttcagcgtg ctgacggagc
    66421 gcgagctgga tgttctgcac gagctggcac aggggctgtc aaataaacag attgcctcgg
    66481 tgttgaatat ttccgagcag acagtaaaag tacatattcg caatctactg cgcaaactca
    66541 atgtccgctc acgcgtggca gccaccattc tgttcctgca acaacgcggg gcacaataaa
    66601 aatagcccga tggcatcacc atcgggcctc tttttattta ctctcctgcg gcgacaaatg
    66661 ttgcatcgcc tgcgcgatac tacgttcaat caccgcacgg cgagtatcgt tggcaggtaa
    66721 gagtttcaac atcatctccc acgcggcaac ggcttcgcca aatcgctgct gctcaaaggc
    66781 attaaacgca tacatgctta acacacgaat attgctatgg tccgttctca ccaactggcg
    66841 cagcagttcg ccgccgaggc ggttgtcgtt gggatcagat gaacgtgtca acgcttcagc
    66901 ataacccagt gtggcatcgc tgtttttcgg atcgaggcga tacgcagtag cgtatgcatc
    66961 ggtggcgata ctggcgttac ccagcgccat gccaacgcga cccaacataa tccaaccttc
    67021 tatatctccc ggattttttt gcagttgagt acgcatcccc agcgcaaggc gcgacatctc
    67081 ttcttcgttg agcggatcgg ctttcggatc cagcgccctg tccagcaacg ccggagcctg
    67141 tgccgtggcc tgctgccaga ttttcacctg ctgataattg ccagtctggt agtagctgac
    67201 gccagccaca attaacgcca ccacaatacc cggcagataa acaacatatc cggcacgctt
    67261 accttccggc acgctttgtt caggaaacgc ttccggtacc acgcgtaccc gacgccgcga
    67321 acgggcgtat atgacccaac cgccaatacc aatagccact accggaagca cccacagcag
    67381 cacggtcagc ggcgttaacg gcggatcgta agtgacgaag ttgccgtaac gcgccaccat
    67441 ataatcgaca atctctttct tacttttgcc ttcctgcatc agttcataca ctttctggcg
    67501 caggtcggtg gcaatcatcg agttggaatc ggcaatgctg ttattctggc atttcgggca
    67561 gcgcagttct tcagtgagct gacggaactg ttgttcctgt gcttcatctt taaactgcaa
    67621 cacgtcgatg gtcgccagcg ctgagccgga gatcatcagc atcagcacgc ccaataaaaa
    67681 cctcattgtg cggcctcctt actgtatttc tcccacagcg gcttgatctc ttcttcccag
    67741 acgcgaggat tgagatcgcc cgcatggcga tagcgaatga tgccgttgcc gtcaataaga
    67801 aacgtttctg gcgcgccata gacaccgaga tccagcccta acatgccatc gccatcaaaa
    67861 aggcttagcg cgtaaggatt gcccagctct ttcagccagc tgattgcctt ctggcgatcg
    67921 tctttatagt tcatgccgac cacgcggatg ccctgcgcag aaagctgatt cagatattga
    67981 tgttccgcac ggcaggtcgg acaccaggtc gcccagacgt taagcagcac tggtttgccc
    68041 tgagtcagca catccgcctg ataaaactgc cccggattgt ccagtgattc gagacgaaac
    68101 ttcggcacag gcttgccaat gagcgccgat tccagattgg tcggatcatc cccttcggca
    68161 ttacgcgcca gctgccacag cagcgccgcg gcaatcgcca ggaagataat caacggaatt
    68221 aacaatactt tgcgcttcat acagcctccg gcgcagtttt ttgcgggctc acgcgcttac
    68281 gatagcgagg atcaaacaga cacagcagtc cgcccaacgc catcatcagc ccgcccgccc
    68341 aaatccagcg aacaaatggt ttgtagtaaa gacgcacagc ccacgcgccg ttttccagct
    68401 cttcaccgag cgcggcgtac aggtcacgcg tgatgccgcc gtcaattgcc gcttcggtca
    68461 tcatcgaccc ggcagtgttg taataacgtt tttccgcata cagcaccgtt tccggtttac
    68521 cctctcgcgt tacgccgata gtcgccacac cgccacgcca gttcgggcca gtcacctctt
    68581 tgacatcacg gaaggtgaag cgatattcat gaatatcgac gctatcgccg gacttcatgc
    68641 gcacatcacg ctcaacgcta tagttctggc taaaggcaat gccaacaatt gtcactgcca
    68701 gccccaggtg agccgccacc atcccccaat aactgaaggt ggttttcgtg ccgcgtgaaa
    68761 tacgtagcgc agcttccgca attgccagca ccgcaatcca gcaggccatt gccaggccga
    68821 gcaccgtcat cgccacaact ttgctttcga acagccacgg caacagcaga gacagcacca
    68881 gcgtagagat gaaggcgata atcaataaat tgcggatttt acgtgggcga tcgcgccccc
    68941 agcgcaccag aggaccgaca cccagcagta gcgcaaacgg caccatcagc caggtaaaca
    69001 tggtgttgaa gaacggttcg ccaatcgaaa tactgcccag tcccagttgc ttgtgcacca
    69061 gcggcagcag cgtccccagc aacaccacca gcatcgcggc gaccagcaaa acgttgttcg
    69121 ctaacagcaa agattcccgc gaccacagcg cattgtttac gcgtgagcga actttgtgtc
    69181 cacgcgcggc aaacagcagc agcgaaccgc caatcaccag caccataaag gcgaggataa
    69241 acataccgcg cgccggatca gacgcaaacg cgtgtaccga taccagcacg ccggaacgca
    69301 cgaggaaagt ccccaacaga cacaacgaga aggcactgat tgccagcagt aatgtccacg
    69361 ctttgaagct ggcgcgttgt tcagtgaccg ccagtgagtg catcagcgca gtccccacca
    69421 gccacggcat aaacgaggcg ttttctaccg gatcccagaa ccaccagcca ccccagccga
    69481 gttcgtaata ggcccatgcg gaaccgagca cgatgccgag cgtcaggaag atccacgctg
    69541 ccagcgtcca cggacgagta aaacgcgcat aagtgctgtc cagacgcccg ctcagcaaag
    69601 aagcaatggc aaaagcaaac gccaccgaga aacccacgta ccccatataa agcagaggcg
    69661 gatggaagat cagccccgga tcctgtaaca gcgggttaag atcgcgccct tcaatcggga
    69721 agttcggcag cgtgcgtgag aatgggttag aggtaaagag aatgaacagc aaaaagccga
    69781 cactgaccat ccccattatc gccagtacgc gggccacaat atccagcgga atacgctgac
    69841 taaaaatcgc caccgcaaag gtccagccgc tcatcagcag cacccacagc agcagtgagc
    69901 cttcatgtgc gccccaggta gccgccacgc gataccacac cggaagctgg gtattggagt
    69961 tgctggcaac ataggtgacg gtaaagtcgt tgaccacgaa agcattgacc agcaccagaa
    70021 atgcgccagc cacagacata aacagcagcc aggcaaacaa gcgggaagac gccatcatgc
    70081 gcgcatctcc gcgcgccacg ccccatagcg gatacacgga cagcagcagc gcaattccca
    70141 gcgccaggca cagcagcccg ttaccaattt ccggcatcat gatgctgggt ccttataaac
    70201 actcgccggg cgacggtggt tagcttccat cgctttctca acttctggcg gcgtgtagtt
    70261 ttcatcatgt ttcgccagca cttcttttgc gaggatatga ttgccttttt ccagctcgcc
    70321 ctgcaccaca acgccctgcc cttcacggaa cagatccggc aaaatgcctt cataagagac
    70381 atccactgag ccttcagcgt cgtaaatggt gaaggtcact ttcagcgaat tgggatcgcg
    70441 ctgcacacta cccggcatca ccatcccgcc aacgcgcaga cgctgaccga cttccggcat
    70501 ctgctgagtt tcacgcttgc cgtagagaat ttcccccggc gtataaaaga gatcgatatt
    70561 cgagcgcagc gcatatagca ccagaccgat agtcagcgcc agccctgcca acacggcaca
    70621 ggcaatccac aagcggtttt tacggcgaat attcatgcag cctcctgctg ttgcgcagca
    70681 cgtaaacgcg cctcacgcgc ccgctgttgc gccacaccac gcagaattgc gcgatgctgc
    70741 atcaccgagt gcacgaccaa aaccaccagc ggaataacgg tcatcaccac cgccagccag
    70801 acaaaaaagg cgtaaccgcc cattgcgaaa aattcattcc aggaagcaaa tgcaggggtc
    70861 atttacggcc tcttttcagt atcagttcac tcacccacgg acggcgtttt tccatcagca
    70921 aaatcaaatt acgcatccgc atcagcgtca gcgtggcaga gaggagcagg aagccaaaaa
    70981 tcgaccagcg cagcggcgaa cgcatcgccg gatcgatact ttgctgcatc cgcgttgatc
    71041 cctgatgcag ggtgttccac cactccacgg agtaatgaat aatcggcaga ttcaccacgc
    71101 caatcagcac caggatacct gccgcacggc ccgccagacg gcggtcgtcg aaggcgtgcc
    71161 acagggcaat cacacccaca tacaaaaaca gcagcaccag ttcagaagtc agacgtgcat
    71221 cccataccca ccaggtgccc cacatcggtt ttccccatgc agagccggta accagggcaa
    71281 taaaggtaaa cacggcacca atgggggcca tcgccgccac cgccaggttg gccattttca
    71341 tctgccagac aaggccaata aacgccgcca ccgccattga tgcataaatg cccatcgacc
    71401 agatcgccgc aggcacatgc aggtagataa tgcggtagct atttccctgc tgataatcag
    71461 ccggagcaaa gccgaatccc cagatccagc cgacggtaag cacgactaca ctggcaattg
    71521 ccagccacgg tataaaccag ccacagattt gatacagccg tggtgggatc gccagttgat
    71581 gcagtgtttt ccacatagtt tcgataccag actcgaacaa aaatcagtaa tccagcgtta
    71641 ttgaatgctg attcgtaacg ctgccgccgt cgcaaaagga cttaatgtcg cggtgcctgc
    71701 cagcaacgcg cctaaaattg ccagataccc gtcaacgggc aaatgcatag aagccgcgtc
    71761 catcgcggcg gtggcaaaga tgagtaatgg gatagtcagc ggtaacacca gtatgctgag
    71821 cagcacacca ccgcgcttaa gtcccactgt cagcgccacg cccggtgcac cgagaaagcc
    71881 aagcgtaggc gttcccagca gtagcgttaa cgccatcact tgccagccat aaacatccat
    71941 tcccagtagc attgctacca gtggcgaaag gatgagtaac ggcagaccgg ttaccatcca
    72001 gtgcgccatc accttcgcca gcacaacggc gggcaagggt aacggcaaca acatcaattg
    72061 ttcaagactg ccgtcctgca aatcgtcacg gaacagtcgt tccagcgcca gcaaggatga
    72121 aagcagcgcg gcaacccaga taatgcccgg tgcaatacgt gccagcagtt gcggctccgg
    72181 accgatactg agcggaaaaa gggtaattac aatcaggaag aaccacagcg ggttggcgat
    72241 ttcagcgcta tggcgaaacg ctacgcgcag ctcaagacgg aaaatgcgcc agaacatcat
    72301 acggccctcg tttgcgtcag tgaaatgcgg cgaattttac tttcagcaac gttgagcggc
    72361 tggtgggtag tgagaatcac aatccccccc tgctctgtat gctgcgccat acgctgggtc
    72421 agacgatcga cgccgttaac gtcaatcgcg gtaaaaggct cgtcgaggat ccataacgtg
    72481 gcacgggtca gccacagacg cgctaaagcg acgcggcgtt gttgcccggc agaaagttga
    72541 tttaccggaa tatcttcgaa tccggcaagc ccggcctgcg ccagggcttc cagacattgt
    72601 gcggtatcgc catcgcgatg ataaaagtgc agattttcta acgccgtcag ccgggttttg
    72661 atccccggct gatggcctat ccataacagg ttttgatggt agctgtcgcg tacctgatgc
    72721 aagggctgcc cttgccagag aacctcgcct gcgtcagggc gagacaaccc cgtcagcaaa
    72781 cggagaagcg ttgtcttccc cgcgccgttg ctaccggtga tttgtaccca ctctcctgcg
    72841 ttcagcgtaa atgacaagcc actaaataag gttcgttcat cccgctcaca aagtaactct
    72901 ctgacttcaa gcatacccac gcaataaccc tgttaaaaac ctggctcgac ttcacgcata
    72961 tcgggcagct tgtgcgctat ccctttatgg caatcaatac aggtttgccc atctttcacc
    73021 gcctggtcat gcatcttcgc ggcaaccgat ttctgggcgg ttgtatccat atactcgaag
    73081 ttgtgacagt tacggcactc ctgcgagtta ttgtccttca tgcgccgcca ctcattctgt
    73141 gccatcgtca gacgatgagc ttcaaatttc tgcggcgtgt caataacgcc aaaaatttta
    73201 ccatacagct ctttacttgc tttgagcttg cgtatcatct tcggcacaaa ctcgtgcgga
    73261 acgtgacaat ccggacaggt cgcacggacg ccgctacggt tgttgtagtg cacggaatcc
    73321 atgtattcct gatacaccgt gttacgcatt tcgtggcagc taatgcagaa ctcttcggta
    73381 ttggcttttt ccatcccagt gttaaagccg ccccagaaga cgatgccgcc gacaaaaccg
    73441 atcaacagca gcgtccccag cgccagacgg ctgggggtac gccaccattt ccacaggcgc
    73501 ttaatcagac caggcttacg gtcagaattt cccataataa cctcttattt cccgtaacct
    73561 tttgatgggg taaaggtatt ccccacgatt ggcgcggtat cggcctgcgg tacgtgacat
    73621 tgcagacaga aataacgacg cggagccact tccgcgccca ctttgccgtc gctgtccata
    73681 aagtgagtag gactgatacg cggcgcgcca gtggtgcgat agctttcgac accgtggcac
    73741 tgcaagcagc ggttggtatt ggtcgttacc tgataacctt caacgctatg cgggatcatt
    73801 ggcggctgat tcacatagtt cagcggcatc cgatcctgct cttttggcat ccgaatggcc
    73861 ccttcctgcg tcccggatac ttccggcgac tggctaaaat cgacgccatt agccgcccaa
    73921 accgcgccgc ttaccaccag ggccagcatc gccgtccatt gacacagcgc tttcttcagg
    73981 tcatggcttt tcatgatttc gctcccgaac tccatcgtgt agttattgta aatacatcct
    74041 cagaacagac atccacgcag cgaccgcacg tcatgcaatc gcggctggtg acctgcaccg
    74101 ggctttgttc atccagcacc ggggcacgta gcacatgcgg ttccgggcaa acatgaaaac
    74161 aatccataca gcggttacat ttctgccgat cgctggccac aacggtaatc acacctttgc
    74221 tacccagcac gccatacagc gcgccaaccg gacaaatgtg cccgcaccag ccgtgttcaa
    74281 cgaccagtag atcaaataaa aacagcgcga gaataagcag cgcgccgctg ccgaagccca
    74341 ttaccaggct acgccccatc agggaaacgg ggttgatcca ttcccagatg agcgtgccag
    74401 ttaaggctga tcccaccaga atgaccacca acagcacgta gcgaatgtgg cgagggatcg
    74461 tcgcagactg attgaggtca aagcgcctgc gtaaccagtt cgctaagtcg gtaatcgggt
    74521 tcagcgggca gacccagctg caaaataatc gctttcctgc cagggcgtag agcacggtga
    74581 taatcaccgc accggtcaac gccaccgtgg caggcagatg accactggca agactttgca
    74641 gcgtcatcag cggatcggtc agcggaacgg tgtcgaataa caggctactg ctatagttgc
    74701 cgtgcaagat ccacacacca aaccacggac cgctcaaaaa catccccagc acgaagaact
    74761 gacaaaggcg acgtaacacc agccaacggt gactgcgcca ccagcctttt ttctccagcg
    74821 cctcgcgccc ggcgtcacgt ttacgatttg ccattgttcc cctccagcca gccgaagcgg
    74881 taatggtgac ctaactcccc tttcgccagt gacagcggta acaccttgat tgccggttgt
    74941 tccagcacgc atactttttc gcacttaccg caaccggtac aggcgtcgct gtgaaccgtc
    75001 ggcagaaagc gggcgtgctt accggtacgc gtgttgcgct ccagctccag ggtgatggcc
    75061 tcatcaattt tcgggcattc gcgataacaa acatcgcagc gcagcccctg aaagttgaga
    75121 cagttttcct ggtccaccag taccaccagc cccatccgcg cgtcgtcgat cgattcaatc
    75181 tcacgatcca gcgcaccgct cgggcacact ttggcgcacg gaatgtcctc acacatttcg
    75241 caaggaatat cccgtgcgac aaaatatggc gtgcccgccg acaggccaga ggccagcgtc
    75301 gccagtttta aggtgtcgta agggcaagcc tgaacacact gaccgcaacg cacacaggca
    75361 ctggcaaagg cgttctcgtt tatggctccg ggcgggcgca accgcacgcc agatgcgcgt
    75421 gcggtttgct gttgtaaccc cagcgccaca cccacggcag ccagcccgcc tgctgtgcga
    75481 acgacatcgc gcagaaagcg gcggcgacca ttttgaggtt tcgctgaccg ggacataatg
    75541 gcttacacct tctccagttt gaccgcgcac ttcttgaagt ccgtctcttt cgagagcgga
    75601 tcggtcgcgt ccagcgttag tttgttaacc agctgtgcgg cgtcgaagaa cggcatgtac
    75661 accaggccct gcggcggacg gttacgaccg cgcgtttcga caatcgagat cacttcgcca
    75721 cggcgagaaa ccactttcac tttgtcgcca cggcgcagat cgcgcgcttt cgcatccagc
    75781 gggtgaataa acaggaccgc ttccgggaag gcacggtgca gttccggcac acggcgagtc
    75841 atactgccgg tgtgccagtg ctccaggacg cgtccggtag agagccacag gtcgtactct
    75901 tcatccggtg cttccgccgc cggttcgaac ggcagcgcga agatcaccgc tttaccatcc
    75961 ggtttaccgt agaacttata gccttcgccc gctttcacgt acgggtcgtt accttcgctg
    76021 taacgccact gcgtttcttt accgttcacc accggccagc gcagaccgcg cgctttgtgg
    76081 tagtcatcga acggcgcgag atcgtgaccg tgaccgcgac caaaccatgc gtactcttcg
    76141 aacagccctt tttgcagata gaagcccagc tcgcgggatt catcgttcag ctgatcttcc
    76201 gccagttcgg ataccgggaa tttgctcact tcgggggtgg catacagaac ttcgtacagc
    76261 gttttgccac gcagttccgg tttcttcgcc agcagctctt ccggccatac ttcttcagtt
    76321 ttgaagcggc gggagaactg gactaactgc cagaggtccg atttcgcttc gcctggcgcc
    76381 tgtacctgct gacgccagaa ctgagtacgg cgttcggcgt taccgtaagc cccctctttt
    76441 tctacccaca ttgcggtcgg caggatcaag tcggcggcca gcgcactgac tgtcggatac
    76501 ggatcggaga cgatgatgaa gttgcgcgga tcgcgccagc ccggcatacg ctcttcatta
    76561 atgttcggcc cggcctgcat gttgttggta cacatggtcc agtaaacatt cagcttgccg
    76621 tctttcagcg cacggtcttg tgctaccgca tgcagaccga ttttcgccgg aatggtgccg
    76681 ctcgggatat tccacttctt ctcgcagata tcacgatgtt tctcgttagt taccaccatg
    76741 tccgcaggca gacggtgagc aaaggtgccc acttcacgcg cagtaccaca cgcggaaggc
    76801 tgcccggtca gggagaacgg accgcaaccc ggctgggaaa ttttgccggt cagcaggtgc
    76861 aggttgtaga ccaggttgtt ggcccacacg ccacgagtat gctggttgaa gcccatcgtc
    76921 cagtaggaga tgactttctt gttcggatcg gcatacagct gcgccagttg ttccagctga
    76981 tcttttggta cgccagtcat ttcagcggtt ttttccagcg tatactcagc aacgaaggct
    77041 ttgtaatctt caaagctcat cggttcggag gcgtcagaac ccggattctt cgctgctttt
    77101 tccagcggat gggtcggacg taaaccgtag ccgatgtccg tcgccccttt gcgcaggtta
    77161 acgtgcttgc tgaagaagtc ctgatttatc gcattgtttt gaatgatata gttggcgatg
    77221 tagttcagga tcaccaggtc agattgcggc gtaaagatga tgccgttatc cgccagctcg
    77281 aagctacgat gctggtaggt tgaaagcacc gccacggtga cgtcctggtt agagagacga
    77341 cggttagtga tgcgtgacca gaggatcggg tgcatctccg ccatgtttgc gccccacagc
    77401 acaaacgcgt cagcctgctc gatgtcgtca tagcagccca tcggctcatc cataccaaag
    77461 gttcgcataa agccaactac tgccgacgcc atacagtgac gcgcattcgg gtcgatgttg
    77521 ttcgaacgga agcccgcttt aaacagcttg gacgcggcat aaccttccca gatagtccac
    77581 tgaccagaac cgaacatacc gatcgattcc ggcccttttt ctttcagggc ggttttgaat
    77641 ttctcttcca tcacatcgaa ggcctgatcc caggtgattg gggtaaattc gccttctttg
    77701 tcatatttac cgtttttcat acgcagcagc ggctgcgtca aacggtcttt accgtacatg
    77761 atcttgggca ggaaatagcc cttaatgcag ttcaggccac ggttaaccgg tgcgtccggg
    77821 tcgccctgac aggccaccac acgcccctgc tgcgttccga ccagaacgcc gcaaccagta
    77881 ccgcagaaac ggcacggcgc tttatcccat ttaatggctt cctgctgacc aacaacggcg
    77941 cgggcaacgc ccggcacgct gagaccggca gccgccgcag cggccgcaac ggcgttagct
    78001 ttcataaagc tacgacgact gagtttcatg gtgtttcctc accttgctct tcctgctggt
    78061 gataaaccag cgacaccgcc agcacgccct ctacgttgcg tactgactca atggtttgga
    78121 tcagcgtttc gctgtcttct gcttccacca ccacaatcaa ctgaccgctc ggcgcgtcgc
    78181 tgacagcaac ttcacagccg ggaaaggcgt tcagttgggt gctgatgtct gaaattcgtt
    78241 cgcttttggc ctgcacaacc aggctgcaaa cttgccagtt agtgtgcatg gagatactcc
    78301 gcagttatgg ctgataccgg acagctggct gcacatgctc cacatccgtt gcaaagttgg
    78361 ctattaagtt gcggctggta gatcccggag agcgtcgggc gaaagataat tgccattggc
    78421 tcacagctat cctgacagcg gcggcattca actgactgat acgccaggca cgcgtcccca
    78481 atggtgaact gcaaatccca ggccctggtg tggcgcggag aaaatagcga ttcggggcaa
    78541 gcctgcgcgc aggcgtaaca gaagctgcac tcgttatttt tgaaattaac gctcggatag
    78601 ccgcccgcgc cgcgttgcag aatgttgttt tcacaagcat taatacaggc gtcgcagcgg
    78661 gtgcaatggg tcagaaaatg agattcatca ccggaccagg gcggacggat accgttactg
    78721 gctttgcgcc agcgaccagt gagtatgccc cgacgggatg catcaatctt cacattgacc
    78781 ttccatcatt aacgcgctct gaaaattgag agcgaccaaa taaaccgcat aattaataag
    78841 ccatttttat agccgctaag atattaaagg atgtgtcaaa gatgcatacc ccgatcgagg
    78901 taaaacctgt agcaggatca aaagagtggc gggaagcgt
//
LOCUS       DAZFTN010000010        74089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFTN010000010 DAZFTN010000000
VERSION     DAZFTN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..574
                     /gene="lpfA1"
                     /locus_tag="ACHKV2_000816"
     CDS             50..574
                     /gene="lpfA1"
                     /locus_tag="ACHKV2_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO6938750.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            639..1325
                     /gene="lpfB"
                     /locus_tag="ACHKV2_000817"
     CDS             639..1325
                     /gene="lpfB"
                     /locus_tag="ACHKV2_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO6938751.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1354..3927
                     /gene="lpfC"
                     /locus_tag="ACHKV2_000818"
                     /pseudo
     CDS             1354..3927
                     /gene="lpfC"
                     /locus_tag="ACHKV2_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3942..4997
                     /locus_tag="ACHKV2_000819"
     CDS             3942..4997
                     /locus_tag="ACHKV2_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6938752.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5002..5532
                     /gene="lpfE"
                     /locus_tag="ACHKV2_000820"
     CDS             5002..5532
                     /gene="lpfE"
                     /locus_tag="ACHKV2_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO6938753.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5787..7478
                     /gene="eptB"
                     /locus_tag="ACHKV2_000821"
     CDS             5787..7478
                     /gene="eptB"
                     /locus_tag="ACHKV2_000821"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO6938754.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7570..7646
                     /locus_tag="ACHKV2_000822"
     tRNA            7570..7646
                     /locus_tag="ACHKV2_000822"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7604..7606,aa:Pro,seq:cgg)
     gene            8391..9998
                     /gene="dppA"
                     /locus_tag="ACHKV2_000823"
     CDS             8391..9998
                     /gene="dppA"
                     /locus_tag="ACHKV2_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO6938755.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10149..11168
                     /gene="dppB"
                     /locus_tag="ACHKV2_000824"
     CDS             10149..11168
                     /gene="dppB"
                     /locus_tag="ACHKV2_000824"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO6938756.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11178..12080
                     /gene="dppC"
                     /locus_tag="ACHKV2_000825"
     CDS             11178..12080
                     /gene="dppC"
                     /locus_tag="ACHKV2_000825"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO6938757.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12091..13074
                     /gene="dppD"
                     /locus_tag="ACHKV2_000826"
     CDS             12091..13074
                     /gene="dppD"
                     /locus_tag="ACHKV2_000826"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO6938758.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13071..14075
                     /gene="dppF"
                     /locus_tag="ACHKV2_000827"
     CDS             13071..14075
                     /gene="dppF"
                     /locus_tag="ACHKV2_000827"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO6938759.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14105..15376)
                     /locus_tag="ACHKV2_000828"
     CDS             complement(14105..15376)
                     /locus_tag="ACHKV2_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO6938760.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15852..15959
                     /locus_tag="ACHKV2_000829"
     CDS             15852..15959
                     /locus_tag="ACHKV2_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6938761.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16046..17725)
                     /gene="bcsG"
                     /locus_tag="ACHKV2_000830"
     CDS             complement(16046..17725)
                     /gene="bcsG"
                     /locus_tag="ACHKV2_000830"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO6938762.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17722..17913)
                     /gene="bcsF"
                     /locus_tag="ACHKV2_000831"
     CDS             complement(17722..17913)
                     /gene="bcsF"
                     /locus_tag="ACHKV2_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO6938763.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17910..19481)
                     /gene="bcsE"
                     /locus_tag="ACHKV2_000832"
     CDS             complement(17910..19481)
                     /gene="bcsE"
                     /locus_tag="ACHKV2_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO6938764.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19754..19942
                     /gene="bcsR"
                     /locus_tag="ACHKV2_000833"
     CDS             19754..19942
                     /gene="bcsR"
                     /locus_tag="ACHKV2_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO6938765.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            19954..20706
                     /gene="bcsQ"
                     /locus_tag="ACHKV2_000834"
                     /pseudo
     CDS             19954..20706
                     /gene="bcsQ"
                     /locus_tag="ACHKV2_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            20703..23321
                     /gene="bcsA"
                     /locus_tag="ACHKV2_000835"
     CDS             20703..23321
                     /gene="bcsA"
                     /locus_tag="ACHKV2_000835"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO6938766.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            23332..25689
                     /gene="bcsB"
                     /locus_tag="ACHKV2_000836"
     CDS             23332..25689
                     /gene="bcsB"
                     /locus_tag="ACHKV2_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO6938767.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            25696..26802
                     /gene="bcsZ"
                     /locus_tag="ACHKV2_000837"
     CDS             25696..26802
                     /gene="bcsZ"
                     /locus_tag="ACHKV2_000837"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO6938768.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGALWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            26784..30257
                     /gene="bcsC"
                     /locus_tag="ACHKV2_000838"
     CDS             26784..30257
                     /gene="bcsC"
                     /locus_tag="ACHKV2_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO6938769.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADEPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDFPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            30339..32327
                     /gene="hmsP"
                     /locus_tag="ACHKV2_000839"
     CDS             30339..32327
                     /gene="hmsP"
                     /locus_tag="ACHKV2_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO6938770.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            32510..33796
                     /gene="dctA"
                     /locus_tag="ACHKV2_000840"
     CDS             32510..33796
                     /gene="dctA"
                     /locus_tag="ACHKV2_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO6938771.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            34017..35513
                     /locus_tag="ACHKV2_000841"
     CDS             34017..35513
                     /locus_tag="ACHKV2_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6938772.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(35609..36538)
                     /gene="kdgK"
                     /locus_tag="ACHKV2_000842"
     CDS             complement(35609..36538)
                     /gene="kdgK"
                     /locus_tag="ACHKV2_000842"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO6938773.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            36770..37537
                     /gene="pdeH"
                     /locus_tag="ACHKV2_000843"
     CDS             36770..37537
                     /gene="pdeH"
                     /locus_tag="ACHKV2_000843"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO6938774.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            37607..39667
                     /locus_tag="ACHKV2_000844"
     CDS             37607..39667
                     /locus_tag="ACHKV2_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6938775.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(39849..41171)
                     /locus_tag="ACHKV2_000845"
     CDS             complement(39849..41171)
                     /locus_tag="ACHKV2_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6938776.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(41583..42596)
                     /gene="yhjD"
                     /locus_tag="ACHKV2_000846"
     CDS             complement(41583..42596)
                     /gene="yhjD"
                     /locus_tag="ACHKV2_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO6938777.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(42645..43544)
                     /locus_tag="ACHKV2_000847"
     CDS             complement(42645..43544)
                     /locus_tag="ACHKV2_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6938778.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            44064..44666
                     /locus_tag="ACHKV2_000848"
     CDS             44064..44666
                     /locus_tag="ACHKV2_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6938779.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(44717..46366)
                     /locus_tag="ACHKV2_000849"
     CDS             complement(44717..46366)
                     /locus_tag="ACHKV2_000849"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6938780.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            46771..48168
                     /gene="ccp"
                     /locus_tag="ACHKV2_000850"
     CDS             46771..48168
                     /gene="ccp"
                     /locus_tag="ACHKV2_000850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO6938781.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            48379..49779
                     /locus_tag="ACHKV2_000851"
     CDS             48379..49779
                     /locus_tag="ACHKV2_000851"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6938782.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            49935..51470
                     /gene="gadC"
                     /locus_tag="ACHKV2_000852"
     CDS             49935..51470
                     /gene="gadC"
                     /locus_tag="ACHKV2_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO6938783.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            51601..52920
                     /locus_tag="ACHKV2_000853"
     CDS             51601..52920
                     /locus_tag="ACHKV2_000853"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO6938784.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            53297..54679
                     /gene="dosC"
                     /locus_tag="ACHKV2_000854"
     CDS             53297..54679
                     /gene="dosC"
                     /locus_tag="ACHKV2_000854"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO6938785.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            54704..57093
                     /gene="dosP"
                     /locus_tag="ACHKV2_000855"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             54704..57093
                     /gene="dosP"
                     /locus_tag="ACHKV2_000855"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            57351..57932
                     /gene="ddpX"
                     /locus_tag="ACHKV2_000856"
     CDS             57351..57932
                     /gene="ddpX"
                     /locus_tag="ACHKV2_000856"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO6938786.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            57946..59496
                     /locus_tag="ACHKV2_000857"
     CDS             57946..59496
                     /locus_tag="ACHKV2_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6938787.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            59498..60520
                     /locus_tag="ACHKV2_000858"
     CDS             59498..60520
                     /locus_tag="ACHKV2_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6938788.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            60517..61413
                     /gene="ddpC"
                     /locus_tag="ACHKV2_000859"
     CDS             60517..61413
                     /gene="ddpC"
                     /locus_tag="ACHKV2_000859"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO6938789.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            61410..62396
                     /locus_tag="ACHKV2_000860"
     CDS             61410..62396
                     /locus_tag="ACHKV2_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6938790.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            62389..63315
                     /locus_tag="ACHKV2_000861"
     CDS             62389..63315
                     /locus_tag="ACHKV2_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO6938791.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(63371..63802)
                     /gene="osmC"
                     /locus_tag="ACHKV2_000862"
     CDS             complement(63371..63802)
                     /gene="osmC"
                     /locus_tag="ACHKV2_000862"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO6938792.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            64147..64362
                     /gene="bdm"
                     /locus_tag="ACHKV2_000863"
     CDS             64147..64362
                     /gene="bdm"
                     /locus_tag="ACHKV2_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO6938793.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            64464..64601
                     /gene="sra"
                     /locus_tag="ACHKV2_000864"
     CDS             64464..64601
                     /gene="sra"
                     /locus_tag="ACHKV2_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO6938794.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            64758..66455
                     /gene="maeA"
                     /locus_tag="ACHKV2_000865"
     CDS             64758..66455
                     /gene="maeA"
                     /locus_tag="ACHKV2_000865"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6938795.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            66589..67599
                     /gene="adhP"
                     /locus_tag="ACHKV2_000866"
     CDS             66589..67599
                     /gene="adhP"
                     /locus_tag="ACHKV2_000866"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO6938796.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            67745..68029
                     /locus_tag="ACHKV2_000867"
     CDS             67745..68029
                     /locus_tag="ACHKV2_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6938797.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(68257..68910)
                     /gene="fdnI"
                     /locus_tag="ACHKV2_000868"
     CDS             complement(68257..68910)
                     /gene="fdnI"
                     /locus_tag="ACHKV2_000868"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO6938798.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(68903..69787)
                     /gene="fdnH"
                     /locus_tag="ACHKV2_000869"
     CDS             complement(68903..69787)
                     /gene="fdnH"
                     /locus_tag="ACHKV2_000869"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO6938799.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(69800..72847)
                     /gene="fdnG"
                     /locus_tag="ACHKV2_000870"
     CDS             complement(69800..72847)
                     /gene="fdnG"
                     /locus_tag="ACHKV2_000870"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(72260..72262),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6938800.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            73079..73960
                     /gene="yddG"
                     /locus_tag="ACHKV2_000871"
     CDS             73079..73960
                     /gene="yddG"
                     /locus_tag="ACHKV2_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO6938801.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT        17963 a        18555 c        19658 g        17913 t
ORIGIN      
        1 aatacatcaa gattttcttt ttaatgtaat tttttaaatg gagtttttca tgaaaaaggt
       61 cgtttttgcc ttaaccgcac tggcgttaac ttctggcact gtattcgcag cagagtctgg
      121 tgacggcacg gttaaattta caggcgagat cgtggattca ccttgcgtac tgtccgttga
      181 ctctcagaac caggaagttg tgttgggtca ggtacaaaaa tctgtatttg ctgcggttgg
      241 tgacaaatcc ccggcaaaac ctttcgaaat caaactggaa gattgcgata ccaccacgat
      301 gaaaaaagcc aatgtgagct ttagtggtgt tggtgatgca gataaatctg atctgatttc
      361 tgttagcact gaagcgggcg cggccaaagg tgttggtatc ggtatttatg ataacagcaa
      421 taccctggtc gctcttaacg gtggtaaagc aagtgttgat ctgagtaaag gccagacggt
      481 tctctacttt accgctaact acgtttctac tctggcaacg gtaacgaccg gttacggcaa
      541 tgcgcaggtc gatttcaacc tgtcttacga gtaatcgtcc gttttacaaa tggcggcgta
      601 attgccgcca ttagtcgtca aagttcatgg atcgaatgat gaaaagtaaa tttgttgcgt
      661 tggctctctc tctgttcctt tcacagtcag ttctggcggg tggcgtcgga cttagctcta
      721 ctcgcgtaat ttatgatggc agtaagaaag aagcctcgct gaccgtacag aataaaagta
      781 agacggaaga gtttcttatc caatcctggg tggatgatgc cgcgggcagc aaaaaaacac
      841 cgtttattat tacgccccca ctattcaagc ttgatccaga aaagaacaat attttgcgta
      901 ttgtgaatat tacgcccggc ctgcctcagg atcgcgaatc ggtctactgg gttaatgtga
      961 aagccattcc atctaaaagt gacgacagcg aaaacaaaaa tgttttgcag attgccgttc
     1021 gcacccgaat caaacttttt tatcgcccgg caggtttaaa gggtgatgta aaaaccgcgc
     1081 caaatgaact gcgatttacg cgtaacggta atcagctccg cgttgataat ccgacagttt
     1141 ttaatattac ctttaatcaa ttttttgcga atgacaaaga aattgaaaaa gcaggcatgg
     1201 tacccgctaa aggggcatta aatatcacat tacccgccgg ggtaggtagc gtttctaaaa
     1261 ttaaatataa caccatcaat gactttggat cgtcagcgga aatgatcact aaaaatgttg
     1321 attgattaat atcttttcag gagaaatagc tcaatgtcac ggaagaccgt ttcacgtact
     1381 ttttcttcct tttctatctc tgtggtggcg gttgctgttg ccagcacgtt ttctgcacat
     1441 gccggaaagt ttaaccccaa attcctggag gacgttcagg gtgtgggcca gcatgtcgat
     1501 ttaacgatgt tcgaaaaagg ccaggaacag cagttacccg gcatctatcg tgtctctgtt
     1561 tacgtcaatg aacagagaat ggaaacccgc acactggaat ttaaagaggc cacagaagca
     1621 cagcgcaaag cgatggggga atcccttgtg ccttgcctta gccggacgca actggcagag
     1681 atgggcgtgc gcgttgaaag ctttccggcc ctcaatctgg tgtctgccga agcctgcgta
     1741 ccgtttgacg aaatcattcc cctggcctca agccatttcg acttcagtga acaaaagctg
     1801 gtattaagtt tccctcaggc ggcgatgcac caggtggcgc gcggcacggt gccggaatct
     1861 ttatgggacg aaggtatccc tgcgttgctg ctggattaca gttttagcgg gagcaacagt
     1921 gaatatgact cgacggggtc ttccagcagt tatgtcgatg ataatggcac tgttcatcat
     1981 gatgatggta aagatacgct aaagagcgac agctactacc tcaacttacg cagcgggctg
     2041 aaccttggcg cttggcggtt acggaattac agtacctgga gccattccgg tggcaaagcg
     2101 cagtgggata atatcggtac gtctttgagt agggccatca tccctttcaa agcgcaatta
     2161 acaatggggg atacggcgac cgcgggggac atttttgaca gcgttcagat gcgtggcgcg
     2221 atgttagact ctgatgaaga gatgctgccc gacagccagc gaggctttgc acccatcgtt
     2281 cgagggatcg ccaaaagcaa cgccgaagtc agtattgaac aaaacggcta cgttatttat
     2341 cgcacctatg tgcagcctgg tgcctttgaa ataaacgatc tttaccccac tgcaaacagc
     2401 ggtgatctca cggtcattat taaagaagcg gatggcagtg aacaacgctt tatttagcca
     2461 ttctcatcgg tgccaatttt ccagcgtgaa ggccatctca aatatagctt tgccgcaggt
     2521 gaatatcagg ccgggaatta tgacagcgcc tcgccgcgtt tcgggcagct tgatctgatc
     2581 tacggtttac cgtgggggat gacggcctac ggcggcgtat taatctctaa taattacaat
     2641 gcatttacat tagggatagg gaaaaacttt ggttatatcg gggcgatttc cattgatgtg
     2701 acgcaggcta aaagcgaact gaataacgat cgcgatagcc agggacaatc ttatcgtttc
     2761 ttatattcca agagcttcga aagcggcacc gatttccgcc ttgcgggcta tcggtactct
     2821 accagcggtt tctatacctt ccaggaagcc accgatgtgc gcagtgacgc tgacagcgac
     2881 tataaccgtt atcacaagcg cagcgaaata cagggtaacc tgacgcagca attaggggcc
     2941 tatggctctg tttatttaaa tttaacgcag caggattact ggaacgacgc aggtaaacag
     3001 aacacggtat cggcgggtta caacggacgt attggcaagg tcagttacag tattgcatat
     3061 agctggaata aaagccctga atgggatgaa agcgatcgct tgtggtcttt caatatttcc
     3121 gttccactag gccgggcctg gagtaactat cgcgtcacga ccgaccagga tggtcgtacc
     3181 aatcaacagg ttggggtcag cggaacgctg cttgaggatc gcaacctgag ctacagtgtc
     3241 caggaaggct acgccagcaa cggtgtgggt aacagcggta acgctaacgt tggctatcag
     3301 ggtgggtccg gtaatgtcaa cgtaggctat agctacggga aagattaccg gcagctcaac
     3361 tacagcgttc gcggcggcgt gatagttcat agcgaaggcg tgacgctttc ccaaccgcta
     3421 ggcgaaacca tgacgctcat ctccgtaccc ggtgcgcgca atgcccgcgt ggtgaataac
     3481 ggcggcgttc aggttgactg gatgggtaac gcgatcgtgc cttatgccat gccgtatcgt
     3541 gaaaacgaaa tctcactgcg tagcgattcg ttgggtgacg atgttgacgt tgaaaatgcg
     3601 ttccagaaag tggtgccaac gcgtggagcg attgtcagag cgcgttttga tacccgcgtt
     3661 ggttaccgcg tattaatgac gctgcttcgt tccgcgggca gcccggtgcc ctttggagca
     3721 acggcaacgc taatcaccga taaacaaaac gaggtgagca gtatcgttgg tgaagaagga
     3781 cagctctata ttagcggaat gccagaggaa ggacgggtat tgattaaatg gggtaatgac
     3841 gcgtcgcagc aatgcgtggc gccttataaa ttatccctgg aattaaaaca gggcggaatt
     3901 attcctgttt cggccaattg ccagtaagca aggagagcga aatgaaagcg gcaatagcat
     3961 tatccttatt aggatgcgta ttcggatttt ctggcaaggc atttgccgga gatgcgtggg
     4021 ggccatgtac ccccgcggat ggcacgactt atcactataa tgtcgatgtg gatgttggta
     4081 tacctgatgc ggcaaaaaac gtcgcaggaa cggttttacc ggatgtcctt aactggtcta
     4141 atggacaaaa cgtatcgcta atttgtgaat gtcctgattc ttataagaat gaaaaagata
     4201 ctctagtgca gggggtgagt atgcttcccc cttctggccg tacggttgac agtatgaaat
     4261 attatacttt gacggaggaa ctggaggttg cgacgaacat tcgcatcagt accagtgtgt
     4321 atgggtttgt tcctttcaaa aaccagcagg cgctgcaaac aaccggatgc aacaaagtca
     4381 ttactacgcc ctatatgggc ggcgcaggtc tactttcctt tgctattact aaacccttta
     4441 tcggcgattc cgtcatccct ctgacgttaa ttgccgaact gtatgcctcg aaaacaaata
     4501 aagattacgg aacaataccc atatcttccg tatctattca ggggcgtgtc accgtaaccc
     4561 aggattgcga aattaaaccc ggcacggtgc tggatgtgcc atttggtgaa ttcccctcct
     4621 cggcgtttaa aaacaggcaa gggcaaatgc ctgaaggtgc gacagagcaa gaaattaacc
     4681 tcagcttcga ttgcaacaat atctctgatg gtatcaaggt tgcgttgcgc cttgaggggg
     4741 caaccaatgc tgacgatccc cgagcggtgg atatgggcaa ccccgatatt ggcgtactgg
     4801 tgaaagattc cagcggcaaa attttggtac cgaatgattc aagcagcacc acgttattaa
     4861 atttgagcag cctggattct aaaactcacc gcaatgccgc gattcgactg ctggctttac
     4921 cgattagcac gacgggtaaa gcgcctaaag gcgggacgtt tgaaggcgta acgaccatat
     4981 atcttgaaat ggagtgatgc catgaagttc aaacgattgc tgcatagcgg catcgccagt
     5041 ttgagtctgg ttgcctgcgg ggtgaatgcg gcgacggatc ttggcccggc aggggatatt
     5101 catttctcca tcactatcac cactaaagct tgcgagatgg aaaaaagcga tctcgaagtc
     5161 gatatgggaa caatgacgct gcaaaaacct gcggcagtcg gtacggtgtt gagcaagaaa
     5221 gatttcacca ttgaactcaa agagtgcgat gggatatcca aagcgaccgt tgagatggac
     5281 agtcagtcgg acagcgatga tgattccatg tttgcccttg aggctggtgg cgcaacgggt
     5341 gttgcgttga agatagagga cgataaagga acgcagcaag ttcccaaagg ctccagcgga
     5401 acgccgattg aatgggcgat tgatggcgaa accacgtcgc ttcactacca ggcgagttat
     5461 gtggtcgtca acactcaggc cactggtggc acagcgaatg cccttgtaaa tttttccatc
     5521 acctatgagt aagttactgg gccagcatcc tggcccaacc tatctctgaa tagtcgttaa
     5581 tccgttaaaa tggtctattc cggacttgca cttttgtaaa tttgtgcttc atgcacactc
     5641 tttccccaca ctttttccct ttgctgtggt ctacttattc gcgcgtgtag attttactta
     5701 tctgactacc tccgcacttt tcccctgctg ggcctgaaaa gccgctaagc agggtgttat
     5761 cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc
     5821 tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttaccgcc
     5881 gcttcggcag ctatgcgcac gattttaccg tctggaaagg catttctgct gttgttgaac
     5941 tggccgccac cgtactggtg accttctttt tactacgtct tctttcgctg tttggccgcc
     6001 gcagctggcg tattctggca tcgctggttg tgctcttttc cgcaggtgcc agctattaca
     6061 tgaccttcct taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata
     6121 tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagcg
     6181 cattgcctct tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc
     6241 gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt actggcggga attatggttt
     6301 gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg
     6361 atttgccgag ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg
     6421 cgttggggct gtacgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta
     6481 acccggcgaa gaaattcact tatcaggcac cgcaaaatgt taatgatact tatgtcgtgt
     6541 ttatcatcgg tgaaaccacg cgttgggacc atatgggcat tttcggctat gagcgtaata
     6601 ccacgccgaa gctggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata
     6661 ccgcaaccaa actctctctg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc
     6721 cgcagcgcac attaaaagaa cagaacattt tcgcggtact gaagcagtta ggattcagtt
     6781 ctgacctcta cgctatgcag agcgaaatgt ggttctacag caatacgatg gcggacaata
     6841 ttgcttaccg tgaacagatt ggtgcggagc cacgtaaccg tggcaagccg gtagatgata
     6901 tgttgctggt agacgaaatg cagcaatcgc tggggcgcaa cccggatggt aagcatctaa
     6961 tcattctgca taccaaaggt tcgcacttta actacaccca gcgttacccg cgcagctttg
     7021 cgcagtggaa gccggaatgt attggtgttg atagcggatg taccaaagcg cagatgatca
     7081 actcctatga caactcggtg acctatgtgg atcactttat ctccagtgtg attgatcagg
     7141 ttcgcgataa gaaagcgatt gtgttctacg cagctgacca cggcgagtca attaatgaac
     7201 gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga
     7261 tgatggtctg gatgtcagat aaatatctgg aaaatccggt caatgcgcag gcgtttgcgc
     7321 agctgaaaaa agcagccgac atgaaagtgc cacgccgtca cgtagagctg tacgacacca
     7381 tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt
     7441 gtcacatccc gcagacaaaa gaggcagcgg ctaactaaga ggcctgctga ctttctcgcc
     7501 gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg
     7561 ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
     7621 aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     7681 tgcatctgca gcccatgagg aagagaacct ccgggggcag ggaggttcga ctcgacgtag
     7741 gcctgataag acgcgtcagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg
     7801 gcgtgaacgc cttattcgac ctacacgcca cacatttccc tgccaaattt gcgccccata
     7861 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     7921 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     7981 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcacta aatgcataaa
     8041 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggtcatgt tggagtagca
     8101 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8161 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8221 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8281 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8341 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8401 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8461 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8521 cgcagctgtt tacctccggt accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8581 tagttgaatt taaaatcggc accaccgaag tgattccggg cctcgctgaa aagtgggaag
     8641 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     8701 ataaagaatt caaaccgacg cgcgaaatga atgccgatga cgtggtgttc tcgttcgatc
     8761 gtcagaaaaa cgcgcaaaac ccgtaccata aagtttctgg cggcagctac gaatacttcg
     8821 aaggcatggg cttgccggag ctgatcagcg aagtgaaaaa ggtggacgac aacaccgttc
     8881 agtttgtgct gactcgcccg gaagcgccgt tcctcgctga cctggcaatg gacttcgcct
     8941 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9001 acctcaaccc aatcggcacc ggtccgttcc agttacagca gtatcaaaaa gattcccgta
     9061 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9121 tctctattac ccctgacgct tccgtgcgtt acgctaaatt gcagaaaaat gaatgccagg
     9181 tgatgccgta cccgaacccg gcagatatcg cccgcatgaa gcaggataaa tctatcaacc
     9241 tgatggaaat gccggggctg aacgtcggtt atctctcgta taacgtgcag aaaaaaccac
     9301 tcgatgacgt gaaagttcgc caggctctga cctacgcggt gaacaaagac gcgatcatca
     9361 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9421 gctataacga cgacgttcag gactacacct acgatcctga aaaagcgaaa gccttgctga
     9481 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9541 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtcg
     9601 gcgtgcaggc caaaattgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
     9661 gcgagcacca gacggtaatg atgggctgga ctggcgataa cggggatccg gataacttct
     9721 tcgccactct gttcagctgc gccgcctctg aacaaggctc caactactca aaatggtgct
     9781 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
     9841 ttgaactgta caagcaagcg caggtggtga tgcacgatca ggctccggca ctgatcatcg
     9901 ctcactccac cgtgtttgaa ccggtacgca aagaagttaa aggctatgtg gttgatccat
     9961 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata cccggtggtg
    10021 ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attccctgcc cgacctgggc
    10081 gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgggtca ctacagagaa
    10141 tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc acgtttatcg
    10201 gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatcct gtgatgatca
    10261 tggcgggcga acgtgggatc tccccagagc gtcacgcgca gttgctggct gaactcggct
    10321 tagataaacc gatgtggcag cagtatctcc attacatttg gggcgttatg catggcgatc
    10381 taggcatttc aatgaaaagc cgaattccgg tatgggaaga gttcgtgccg cgctttcagg
    10441 ccacgctgga acttggcgtc tgcgcgatga tttttgcgac cgcggtcggc attccggttg
    10501 gtgtgctggc cgcggttaaa cgcggttcca ttttcgatca cacagcggtt ggcctggcgc
    10561 tgacaggtta ttcaatgcct atcttctggt ggggcatgat gctgatcatg ctggtttcgg
    10621 tgcactggaa cctgacaccc gtctccggtc gcgtgagcga tatggtgttc ctcgatgatt
    10681 ccaatccgtt aaccggtttt atgctgatcg acaccgccat ctggggtgaa gacggtaact
    10741 ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact attccgctgg
    10801 cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggtgaa gattacatcc
    10861 gcaccgcgcg cgccaaaggg ttgacccgca tgcgggtgat tatcgtccat gcgctgcgta
    10921 acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg ctggcggggg
    10981 cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg attgacgcac
    11041 tgcaacggcg cgattatccg gtggtgcagg gcggcgtatt gctggtggcg acgatgatta
    11101 tcctcgtcaa cctgctggtc gatctgctgt atggcgtggt gaacccgcgt attcgtcata
    11161 agaagtaagg ggtcatcatg tcacaggtta ctgaaaataa agtgattagc gcgccggtac
    11221 cgatgacccc tttacaggag ttctggcact attttaaacg caacaaaggc gcggtcgtcg
    11281 ggctggttta cgtcgtcatc gtgctgttta tcgcgatctt tgccaactgg attgctccct
    11341 ataacccggc ggaacagttc cgcgatgcac tgctcgcccc gccagcctgg caggaaggcg
    11401 gcagcatggc gcacttgctg ggtaccgatg atgtaggccg tgatgtgctg tcgcggctga
    11461 tgtacggcgc gcgcttgtcg ctgctggttg gctgtctggt ggtggtgtta tcgctgatta
    11521 tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggtcgat aacatcatta
    11581 tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg gtgctggtgg
    11641 cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gacctttgtt gccttgccgc
    11701 actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg
    11761 cttctcgcgt ggcaggagcc ggggcgatgc gccagatgtt tattaacatc ttcccgaact
    11821 gccttgcacc gctgattgtt caggcgtcgc tcggtttctc taacgccatt ctcgatatgg
    11881 ctgctcttgg tttcctcggc atgggggcgc agccgccaac gccggagtgg ggcaccatgc
    11941 tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc ccgggtctgg
    12001 cgatcctgct gacggtgctg gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg
    12061 atcccaaact gaagcagtaa gaggttcgag atggcgttat taaatgtaga taaattatcg
    12121 gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcattag ctacagcgta
    12181 aaacagggcg aagtagtcgg gattgtgggt gagtctggct ccggtaagtc ggtcagttcg
    12241 ctggcgatta tggggctgat tgattatccg ggccgcgtga tggcggaaaa gctggagttt
    12301 aacggtcagg atttgcagcg tatctcggaa aaagagcgcc gcaacctggt gggtgccgaa
    12361 gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac cgtgggtttc
    12421 cagattatgg aagcgattaa ggtgcatcag ggcggtaata agagcactcg tcgtcagcgg
    12481 gcgattgact tgttgaatca ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac
    12541 ccgcatcagc tttccggcgg catgagccag cgcgtgatga ttgcgatggc cattgcctgt
    12601 cggccaaaac tgctgattgc cgatgaacca accactgcgc tggacgtgac cattcaggcg
    12661 caaatcattg aactactgct ggagctacag cagaaagaga acatggcgct ggtgttaatt
    12721 acccatgacc tggcgctggt ggcggaagcg gcacataaaa tcatcgtgat gtatgccggt
    12781 caggtggtgg aaacgggcga tgcgcacgcc atcttccatg cgccgcgtca cccgtatact
    12841 caggcattgc tgcgtgcgct gccggaattt gctcaggaca aagaacgtct ggcatcgttg
    12901 cctggcgttg ttcccggcaa gtacgaccgc ccgaacggct gcttgcttaa cccgcgctgc
    12961 ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct cgctgacggg
    13021 cgtcagtcca aatgccatta cccacttgat gatgccggga ggccgacact atgagtacgc
    13081 aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa cattatccgg
    13141 tgaagaaagg tatgttcgct ccggaacgtc tggtgaaggc gctggacggc gtttcgttta
    13201 accttgaacg tggcaaaacg ctggcggtag taggtgaatc tggctgcggt aaatcgaccc
    13261 tcggtcggtt gctgacgatg attgaaacgc ccaccggcgg cgagctgtat taccaggggc
    13321 aggatctgct taagcacgat ccgcaggcgc agaagctgcg tcggcagaaa atccagattg
    13381 tcttccagaa cccttacggt tcgctgaatc cgcgtaaaaa aatcgggcaa attcttgaag
    13441 agccgctgct gatcaacacc agcttaagca aagaacagcg tcgggaaaaa gccctgtcga
    13501 tgatggcgaa agtcggcctg aaaaccgagc actatgaccg ctatccgcat atgttctccg
    13561 gcggtcagcg tcagcgtatc gccattgccc gaggtctgat gctcgacccg gacgtggtga
    13621 ttgccgatga accggtctcc gcgctggacg tctcggtgcg tgcacaggtg ctcaacctga
    13681 tgatggattt gcagcaggaa ctggggcttt cttatgtctt catctcccac gacctgtcgg
    13741 tggtggagca cattgctgat gaagtgatgg tgatgtacct gggccgctgc gtggagaagg
    13801 gaacgaaaga ccaaatcttc aataacccgc gccatccgta cactcaggcg ctactttccg
    13861 cgacgccgcg cctgaatccg gacgatcgcc gcgagcgcat caaactcacc ggtgaactgc
    13921 caagcccgct gaatccaccg ccgggctgcg cgtttaacgc ccgctgtcgt cggcgcttcg
    13981 gcccctgcac ccagttgcag ccgcagctaa aagactacgg cggtcaactg gtagcttgtt
    14041 ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg ccatgcgctc
    14101 cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg taaagcagaa
    14161 tcagccaggt tttaaatccg cgaagttttt ccagttgtgc gactttatag atcaggaaaa
    14221 acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag aacaccagca
    14281 ccgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg cagatcgcca
    14341 gagtcagcac gcgtgagtta attttcttgg tatcaataat tcggcttaac agattgagaa
    14401 taatgccttt aatggcctcg tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg
    14461 caaagatatt cagcaccgta gaggtgatat gaatgatatg cccagggatc acctgcgcgg
    14521 ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg tggctaattg
    14581 agaaggtaaa cgaaaaggca aaaaacagta tcaccgcgat gagcgtgata taactaattc
    14641 ggtgggtacg cagcgcgagc cgggttgcca gtactttatc cgcttcccgt ttacgatagg
    14701 caatattcat tgggttaagt acctgaataa atactgcaga aaagaagcaa aatggaatgg
    14761 taagcaggac atcgcgaaaa aagacggagg cttgcgggaa ggcggttata ttggcgaaat
    14821 tccagtgcgg gatcatcgta aaaccgaaca cgacaataat ccctactttg accaccacca
    14881 ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct accagtacgg
    14941 cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg
    15001 ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa atcccgtgga
    15061 taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc aggtaatgac
    15121 tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt aaataaatgt
    15181 cctgcactac ccaggtggca ggataagcaa tgatcgctgc ggtaataaat acccaaatgc
    15241 ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc atgcctatgc
    15301 ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgatcg tgtttcggta
    15361 gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa
    15421 cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc
    15481 aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg catttcacta
    15541 ccagtctgga gtcagcccgg atatgaactg caactattgc aaattaagaa attaagctgg
    15601 aaggcatcac gcaatgagga agattgccga gtgctgacag taaaaagcaa tcgtaaaatt
    15661 agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg
    15721 cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacaccac
    15781 gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt cattacaaca
    15841 caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat ttagcggctc
    15901 cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
    15961 gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag accgcagggg
    16021 ggaaactctg cggccttttt cgtgcttact gcgggtaagg cacccagtcg ccgccgttca
    16081 ggcgaacgta cggtttatcc tggtattgaa taactactgc atttgagttc tctgagaccg
    16141 gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa
    16201 tcttgccatc aagaacgcga accaccagat cggagatagc caggaagctg ctcggttggt
    16261 cgatgacaat cggtgccccc tgatgtggtg ccttcatgcc gaagaatttc accccaacgg
    16321 ggacgtcggt gatagacggg ctagggatat cacgtaggcc agatacctgc attctgtcgc
    16381 ccttcagcgc gccgccgtgt tccggcacca cgaccaccat cactttacga cccgatttct
    16441 ccagttcagt aaagaaggcg tccagttcat caaagaattt ctgcgcccgc gctttgtaat
    16501 ccgctgtttt gctgacgccc ggataatggt tgccgtcatg cagtggaagc gtgttgtaga
    16561 acgtggcact acggctgttt ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg
    16621 cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa aataaccggc agatttgttt
    16681 gatccatcaa ttcagtctgc atgccgccat tttcgcgaac ttctttcaaa aaaccgccga
    16741 actggccgtt atgtcccatc atcaggtgct gggtaaagcc cagtttcgac aggttatcaa
    16801 acagatagca gtcgttattt gccggttgat acagattagt gtgcgaagtc tgcccgcagc
    16861 tggcgcgcag taaacggatc gccgccgggc cactgtagga ggtggcggag ttaaagttct
    16921 tgaactcaat atcgaaatgc gaccacagtg gatgcgacat caacccagcg gcttctatat
    16981 ccgaccagga aagcgaacag atgttaatca ccagtagttc aaatggctga gcatcagcgg
    17041 gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc gttatagaaa ttattcagcc
    17101 aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc gggcatatca cccactaccg
    17161 gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc acccgtcgtt gttacagtgg
    17221 tcgtcggttg tccggctggc cacaaggaga aacttggtcc cgccagggta agtacgttca
    17281 gccatagcag tatggcaacc acaaaaacgg taatgcgaat ccattgtgac aggaataacc
    17341 aggccactaa taaaacaaaa atggccccaa tcatctgcca gttaataaag cgtgtgacaa
    17401 ggtcgattaa ataatcggta ctgaaccccg ccacctgcga accctggctc attatgcttt
    17461 ccgggccagg caaccaggtg tcatgccaga acaaagcaaa gccgatcggc agggcaatcc
    17521 agtggcgcaa gcgatgcagg ctgtagcgcg gaatgggcat cagcagaaac gcggcaaaca
    17581 ccaaattgag gagcggatgg aagttaagat atcccgccca caacaggccg aacttaacca
    17641 gaaaatagaa gttccagccg gaaaggccgc gccagtattg ccagagggaa gaaggcatgg
    17701 cggtattttg cgtaaattga gtcatttttt ggttgccctg gctttttccg tgcggcgtaa
    17761 cgtcccagcc ggtttaacat aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat
    17821 gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa aatatcagtg cgcaaacgac
    17881 aataatttca atgatatcgc tgatggtcat catgatgagc gctccacagc atcatctaac
    17941 agtcgcatgg gttctggtat tcgtcgccag tggcgaccat cgtgctcggc attgatgacc
    18001 ggtttagaac tttgcgttaa aggcagcggc atgccccatt gttctggggc aagcaggcgc
    18061 atctgcacca gctcggcact gatttgatca tcttcaaacc agaccatacg gtttgagaaa
    18121 atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg tatccagatc gttaatccga
    18181 cagaatgaga gaaacagcac cagccgatta ccgccaatgg tcatgatatc gccggtacgg
    18241 ttagggcgac acagcgtcag ggcttgttca acgcggatac ccggtaccgg acgtagggca
    18301 accagaacgc ctttaccgtg ggcaggtaat agagggttat tcatcatgtt gttgacggca
    18361 ttacagaaca catcccactt ctggaaacca cgcagtttga gcggctgggt cattgacagc
    18421 aaggtagtga tatcttccgg cacatagcga ctaaacttct gcccttgcac gctttcgatc
    18481 atcgtcagac aacgggagag tggcgcattc cacggaataa ccatatttgc accgcaggcc
    18541 aataacaaac gttcatcggt ggcgcgcagg ctagcggtat tttcccgcac gaggattttc
    18601 atcgcactac cgcgctggcg acgcagggta tgaatgctgc gggccagtgg ctcgatttgc
    18661 gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag cggtacgggc ttcattgaac
    18721 aggacttcgt tattgttgaa cagttgccag tgttccgata gcggcggcgc accttccagt
    18781 acagcaacat tactcagaat gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg
    18841 ctttgaacta atgtccagat accattttgt tgctgaacgc taagctgctg acgggcgctg
    18901 accccttttt cgttgcacca gaaggcaata tccagcaaat gttggtcgcc ctgaaaacgc
    18961 aaactggcaa gaccaaaaag tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg
    19021 ttattatttc cgggattaat taccaaaagc gaacaatggt ttaacctgct ccatttattc
    19081 attttatcca accatgagcg cagccgctcg gcaggaatgt tttgccatgc gttatttgcg
    19141 caaacaagaa taaaaaggta gttatgggga tcaatagaac actgcaaatc acgggccaaa
    19201 tagtatagac ctttttcatg atttagcatt gaaaataatt ttattttttc cggcccttga
    19261 gaatcatcta attgaaagat tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt
    19321 gcggtttcag cctgggatgc aattgtttga ttcgccagac tgatagcatc ttcatggcga
    19381 tcgacgttaa accaccagac gccgcctgct ggcatatgtc gcagctcatc ccataatgat
    19441 gagataccga tagagaatac agggtccaca atgtccctca tgtcaccatt ttgtctgatt
    19501 atcagtttac tagcgaaagc taagaaataa acctaccatt gaaattaaaa cttatcagat
    19561 ttagcatgtg aatcaattta gcaggtcaca agacgtgtga ttttagtctt ttcgttttta
    19621 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
    19681 aataaaccgg cgaaatgctc acggttagaa ttgtctcatg aacggtacgg ttatttcata
    19741 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
    19801 tatcttccag aatgatattt tggcgttaaa gcaggcattt tcactgcctg gtattgatta
    19861 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
    19921 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
    19981 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
    20041 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
    20101 gttgatttta cccaccgtca gggctgggcc agagctatgc tggatggcta ggactggcgc
    20161 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
    20221 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
    20281 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
    20341 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
    20401 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
    20461 attaataact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
    20521 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
    20581 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatattg
    20641 acgctggcga actggtgcct gttgaactat tccgggctga aaacgccagt cgggagtgca
    20701 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
    20761 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
    20821 ggatgatcct ggcctggatt tttattccac tggagcaccc gcgctggcag cgtattcgcg
    20881 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
    20941 tccgttatct cattcaaaca tgctggttac tgatcggtgc atcgcgcaaa gaaacgccga
    21001 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaatgga
    21061 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
    21121 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
    21181 tgattctggc gttaatctgc gttactcagc cgtttaaccc gctggcgcag tttatcttcc
    21241 tgatgctgct gtggggggga gcgctgatcg tacggcggat gccggggcgc ttctcagcgc
    21301 taatgttgat tgtgctgtcg ctgactgttt cttgccgtta tatctggtgg cgatatacct
    21361 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
    21421 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
    21481 gtcagccggt gccattgccg aaagatatgt cgctgtggcc gtcggtggat atctttgtcc
    21541 cgacttacaa cgaagatctc aacgtggtga aaaataccat ttacgcctcg ctgggtatcg
    21601 actggccgaa agacaagctg aacatctgga tcctcgatga tggcggcagg gaagagtttc
    21661 gccagtttgc gcaaaacgtg ggggtgaagt atatcgcccg taccactcat gaacatgcga
    21721 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
    21781 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac cgtgggctgg ttcctgaaag
    21841 agaaacagct ggcgatgatg cagaccccac accatttctt ctcgccggac ccgtttgaac
    21901 gcaacctggg gcgttttcgt aaaacaccga acgaaggcac gctgttctat ggtctggtgc
    21961 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctgtgcg gtgattcgcc
    22021 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac tgtgactgaa gatgcgcata
    22081 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
    22141 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
    22201 ggatggtgca aatcttccgt ctcgataacc cgctcaccgg taaagggctg aagtttgctc
    22261 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
    22321 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
    22381 tgatgatcgc cctgttcgtg ctgccgcata tgatccatgc cagcctgacc aactccaaga
    22441 tccagggcaa atatcgccac tctttctgga gtgaaatcta cgaaacggtg ctggcgtggt
    22501 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
    22561 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
    22621 tcttccttgt tctgctcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
    22681 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtattc tacaacctga
    22741 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
    22801 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
    22861 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
    22921 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
    22981 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
    23041 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
    23101 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
    23161 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
    23221 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccgcccg gagcggagcg
    23281 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
    23341 aaactattct ggatttgtgc agtggctatg gggatgagtg cgttcccctc tttcatgacg
    23401 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
    23461 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
    23521 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
    23581 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
    23641 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
    23701 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
    23761 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa tccactgttt
    23821 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
    23881 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
    23941 tatcagaccc tgaatgtgaa gaatgacctg tcacacttcc cggtgccatt ctttgacccg
    24001 cgtgataacc gcaccaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
    24061 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
    24121 aacttcccgg tgctctataa ccaactgccg gatcgtaatg ccattgtctt tgcaaccaac
    24181 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
    24241 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
    24301 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
    24361 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
    24421 gtgcgtaccg atcgtccggt cacctttggt gaactgaaaa cctatgaaga acagttacaa
    24481 tccagcggtc ttgagccagc agcgattaat gtttcgctaa acctgccgcc agatctctac
    24541 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
    24601 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
    24661 agcagcaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
    24721 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
    24781 ttcgactttg agtatatgaa cccgatgcct ggcggttcgg tggataactg tattaccttc
    24841 cagccggtgc agaatcatgt ggtgattggt gacgactcca ccatcgactt ctcgaagtat
    24901 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
    24961 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
    25021 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
    25081 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
    25141 atcggtggta tcccggacaa actgaaagac gataagcaga tcgacctatt ggtgcaggcg
    25201 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
    25261 gagagcgatc gcgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
    25321 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
    25381 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
    25441 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
    25501 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
    25561 ccgattctgc tggcggtgct ggcggtgctg gcggtgctgg cggctatcag tgtgatattg
    25621 ctggcatggg tactgtggcg tttgctgcgt attatcagcc gtcgtcgtct taacccggat
    25681 aacgagtaat tgaagatgaa tgtgttgcgt agtggactcg tgacgatgct gctgttggct
    25741 gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa aaaggattac
    25801 atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac cacctctgaa
    25861 gggcaaagtt acggcatgtt ctttgccctg gcggctaacg accgtgcagc tttcgataat
    25921 attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg tttgcccgcc
    25981 tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc
    26041 gatggtgatg tctggatggc ttggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag
    26101 cgttataccg acatcggcag cgcgttgcta aaacgtattg cgcgggagga agtggtgacg
    26161 gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga ggataacagc
    26221 tggcgtttta accccagcta cctgccgccg acgctggcgc agtatttcac ccgctttggc
    26281 gcgctgtgga ctacgctgcg cgaaaccaat caacgtttat tgctggaaac cgccccgaaa
    26341 ggcttttcgc cagactgggt gcgctatgag aaagacaaag gctggcagct aaaagccgaa
    26401 aaaacattga tcagcagcta cgacgctatc cgcgtttaca tgtgggtagg catgatgcct
    26461 gacagcgatc cgcagaaagc gcggatgctc aaccggttta aaccgatggc gacattcact
    26521 gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa
    26581 ggaccggtcg gtttttctgc cgccatgctg ccctttttac aaaaccgtga tgcgcaggcc
    26641 gttcagcgcc agcgcgtggc cgataacttt cccggcagcg atgcctatta caactatgtg
    26701 ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag
    26761 ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc acgctttccc
    26821 tcggtctggc cgtcatgccg atggtcgagg cagcaccaac cgctcagcaa cagttgctgg
    26881 agcaagttcg gttaggcgaa gcgacccatc gtgaagacct ggtgcagcag tcgttgtatc
    26941 ggctggaact tattgatccg aataacccgg acgtcgttgc cgcccgtttc cgttctttgt
    27001 tacgtcaggg cgatattgat ggcgcgcaaa aacagctcga tcggctgtcg cagttagcgc
    27061 cgagttcaaa tgcgtataaa tcgtcgcgga ctacgatgct actttccacg ccggatggtc
    27121 gtcaggcact gcaacaggca agattgcagg cgactaccgg tcatgcggaa gaagctgtgg
    27181 cgagttacaa caaactgttc aacggtgcgc cgccggaagg tgacattgct gtcgagtact
    27241 ggagtacggt ggcgaaaatt ccggctcgcc gtggcgaagc gattaatcag ttaaaacgca
    27301 tcaatgcgga tgaaccgggc aatacgggcc tgcaaaacaa tctggcgcta ttgctgttta
    27361 gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca gatggcgaaa tcgaacgccg
    27421 ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa agacatgccc gtcagtgatg
    27481 ccagtgtgtc ggcgctgaaa aaatatcttt ctatctttag tgatggcgat agcgtggcgg
    27541 ctgcgcaatc gcaactggca gaacagcaaa aacagctggc cgatcctgct ttccgcgctc
    27601 gtgcgcaagg tttagcggcg gtggactctg gtatggcggg taaagccatt cccgaactac
    27661 aacaggcagt gcgggcaaac ccgaaagaca gtgaagctct gggggcgctg ggccaggcat
    27721 attcacagaa aggcgatcgc gccaatgcag tggcgaatct ggaaaaagcc ctcgcactgg
    27781 atccgcacag cagcaacaac gacaaatgga acagtctgct gaaagtaaac cgctactggc
    27841 tggcgatcca gcagggcgat gctgcgctga aagccaataa tcctgaccgg gcagaacgcc
    27901 tgttccagca ggcgcgtaat gtcgataaca ccgacagtta tgcagtgctg gggctgggcg
    27961 atgtggcgat ggcgcgaaaa gattatcccg ccgccgaacg ttattatcag cagaccttgc
    28021 gtatggacag cggcaacact aacgccgtgc gcgggctggc aaatatttac cgccagcaat
    28081 cgccagaaaa agctgaagcg tttatcgcct cactctctgc cagtcagcgg cgtagcattg
    28141 atgatatcga acgcagcctg caaaacgacc gtctggcaca gcaggcagag gcactggaaa
    28201 accagggcaa atgggcgcag gcggcagcac ttcagcggca acgactggcg ctggaccccg
    28261 gcagcgtatg gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc
    28321 aggccgatac gttaatgcgc aatctggcgc agcagaagcc gaacgacccg gagcaggttt
    28381 acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg gcgcatatca
    28441 acagcctgcc gcgtgcgcag tggagcagca atattcagga gctggttaat cgactgcaaa
    28501 gcgatcaggt gctggaaacc gctaatcgcc tgcgagaaag cggcaaagaa gctgaagcgg
    28561 aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
    28621 cgctgcagcg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
    28681 agccaactaa cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca
    28741 cagcggcggc acgtagccag ctggcgaaac tgcccgctac tgataacgcc tcgctgaaca
    28801 cacagcggcg cgtggcgctg gcgcaggcgc agcttggaga taccgcagcg gcgcagcaga
    28861 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
    28921 tgctgcgcga cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa
    28981 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
    29041 cctttacccg actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca
    29101 gcgatgcggc ggacctctat cgccagcagg atcttaacgt caccctcgag catgattatt
    29161 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
    29221 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
    29281 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
    29341 tacgggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
    29401 ggcgaaatga cgtctggagc tgggatatcg gcaccacgcc gatgggcttc aacgtggtgg
    29461 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
    29521 cccaccgtcg tcccatctcc agttctttgc tggcctttgg tgggcaaaaa gacttcccga
    29581 gcaataccgg gaaaaaatgg ggcggcgtgc gtgccgacgg cgtggggcta agcctgagct
    29641 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    29701 aaaatgtcga agataactgg cgtgtgcgct ggatgacggg ctattactat aaggtcatca
    29761 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    29821 acctgagtgg ctactcactc ggtcagggcg gttactacag cccgcaggaa tacctgtcgt
    29881 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    29941 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    30001 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    30061 gcttcggcta tacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgtcg
    30121 gcacggcaat tgatatccag caggcgaaag attacgcccc cagccatttc ctgctctacg
    30181 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    30241 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    30301 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    30361 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    30421 ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa
    30481 gcattgctcg ctctgtccgc gaacctttat cttcagccat tttgaaaggt gatattcccg
    30541 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    30601 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg
    30661 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg
    30721 aacgtccggc aaatccgcag ccaattgcct atctggtgct acaggcggat tccttccgta
    30781 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgt
    30841 taatattgac ggtggcgatt agctggtgca ttaaccgcct gattttgcat ccgttacgca
    30901 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    30961 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    31021 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    31081 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    31141 aaaccaccgc gctgatgatc atcacctgtg aaactctgcg tgatactgcg ggcgtgctga
    31201 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    31261 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    31321 tacatgaacc gtggcacgca atcactttgg gtcagcaagt gctcactatc atgagcgagc
    31381 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattagcgtg gcgatgttct
    31441 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    31501 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    31561 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgctattt
    31621 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    31681 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    31741 gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    31801 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gtgctgcaac
    31861 tgatgcaccc gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    31921 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    31981 tggcaatcct ccgtccgctg cgtaatgccg gagttcgggt ggcgctggat gatttcggca
    32041 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgttctga
    32101 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    32161 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    32221 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgtc
    32281 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    32341 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    32401 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    32461 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    32521 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    32581 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    32641 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    32701 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    32761 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    32821 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    32881 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    32941 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    33001 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    33061 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat
    33121 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    33181 ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    33241 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    33301 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    33361 ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    33421 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    33481 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    33541 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    33601 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    33661 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    33721 actggaccac aaaaaactgg acgatgcgct gaataatcgt gcgccggatg gcaaaacgca
    33781 cgaattatcc tcttaatctc atcacttatg cccgcactcc cttccccgag tgcgggtgtt
    33841 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    33901 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    33961 gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc
    34021 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    34081 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    34141 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    34201 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    34261 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    34321 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    34381 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaggcg ctctcttatc
    34441 tggcaaatgc cactggcaaa ctgactatca cgccagaaac catcaaccac gcgctgcaaa
    34501 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    34561 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    34621 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctgctgg
    34681 tggtgggaaa cgtggatgcg cgctcggtcg tcgaccaaat caacaaaacg tttggcgaac
    34741 tgaaaggcaa acgtgaaaca ccggctccgg tgccgacgct ttctccgctg cgtgcggaag
    34801 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    34861 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    34921 gtgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    34981 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    35041 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    35101 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    35161 tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg ggccagcgga
    35221 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    35281 aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt cagcagttgt
    35341 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    35401 cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg accacctctg
    35461 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    35521 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaactc
    35581 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    35641 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgct gcgttttccg
    35701 cactgccgcc cgtcaaacgt accgccagat aaccggcact gaaagagtcg ccagctgcgg
    35761 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    35821 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    35881 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    35941 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    36001 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    36061 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    36121 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    36181 ccgcggattg atcgctctcc agccagaatt tggcggcggc ttcgttacgc cagtagtaga
    36241 acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    36301 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atctgctggc
    36361 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    36421 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    36481 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    36541 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    36601 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    36661 ttgtggagtg gggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    36721 atctttgtcg agtccgggct gcatcacttt taaacacagg acatctttga tgataaggca
    36781 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    36841 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcgggcggtt
    36901 aatggccgtg gagctattaa cggcggtcac gcatcccttg aacccttcgc aacgcctgcc
    36961 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    37021 gattgatttg ctggcgcaaa aagccgactt ctttatagag cacggcctgc tggcatcggt
    37081 caatattgat ggtcctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    37141 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    37201 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat
    37261 ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact
    37321 gtttgtgatg ctgcgtcagt cgccggaagg acgcacactc ttttctcagc ttttacatct
    37381 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacgccgg aagagtggcg
    37441 tgatgtacag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    37501 gatagaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgcctggct
    37561 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    37621 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    37681 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    37741 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaagca
    37801 agaaactggc tggcgcagct gggtgccgtg gccccatgta cacgcggaag acatcattct
    37861 tggcaatcca ccggatattc ccgaagtcac gatggtgcat ttgccacgcg tagaagcaac
    37921 gctggccccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    37981 gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatctcgc
    38041 caacgatgat aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    38101 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    38161 gatttttgtt gatcccttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    38221 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    38281 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    38341 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    38401 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    38461 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    38521 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    38581 tcgcggcttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    38641 cggcaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    38701 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    38761 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    38821 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    38881 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    38941 cttgcgcctg caaccgctga aatttggcat tgcgggtggc agcattgcgg cgaatattca
    39001 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    39061 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    39121 tgacgcggaa ctgcgcggta gcggtaactc ggtggcggca cttttaggca acagtaacgg
    39181 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    39241 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    39301 ctgcgcggcg gcgaatctga atattgccaa cggcgtggca cgcccgcaga tttttgcttt
    39361 cgatactgag aacgcgttga ttaacgttac cggcacggca agttttgctt cggaacagct
    39421 ggatttgact attgatccgg agagtaaagg tattcggatt atcacactgc gttcgccgct
    39481 gtatgtgcgt gggacgttta aaaatcctca ggctggggtg aaagccgggc cgttgattgc
    39541 ccgtggtgct gttgctgcgg cactggcaac gctggttacg ccagcggcag cgttgctggc
    39601 actgatctca ccttccgaag gggaggctaa tcagtgccgg acgattttgt cgcagatgaa
    39661 gaagtgaaga taattgccgg atgtctgaca tccggcaatt aaacagattt agattgttgg
    39721 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    39781 tgcgaattca atatattgta tggaaattca ggcctgataa gcgtagcgca tcaggctttt
    39841 cactcttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    39901 aagccagcca tcgccgccag atataacccc accgcactta gcccgtagtt agtctgcaac
    39961 caggctgcga tatatggcgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaac
    40021 gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccattggc
    40081 ccgaaggtca gacccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    40141 ttgccagaac cgagcagtgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    40201 atgattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccagc gactggcacc
    40261 atcacgccaa aaccaataac tgccatcatc aacatccaca acacttcgtt acgcggcagg
    40321 ccaagcccaa ctggcgcggc ggcggtacta aaggtcatag agtagaccgt catgatgtaa
    40381 aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg
    40441 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    40501 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag
    40561 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    40621 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgcgggaag
    40681 gagccgtaca atgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    40741 ccccattcac cacctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    40801 gcgaaaatac caatcgtggc atagcctggc agcagaccaa tcaccacggt cgaaatcccc
    40861 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    40921 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    40981 agcgttgctg ccgtaggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    41041 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    41101 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    41161 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    41221 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    41281 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    41341 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    41401 agttggcatg aaattgtttg ctcctgaata gatggctgaa gaggctctgg gaaagagttt
    41461 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat tcggcctacg
    41521 gggagccagg ccgaataaat tcgtatgccg gataaggcgt tttgaactgc atccggccta
    41581 ttttaaggct gcgttttccc cggcattcgc gggtcgtctt tatattcggc ggtggcaatc
    41641 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    41701 acggaaccaa atgctgcgcc agagggggat ttcatcaacg atggcagggt gtaggtcatc
    41761 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    41821 ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag atagttggcg
    41881 aagatggaaa tcgccaggcc aatcaaccgc cacgtcggtt tcagccactc aatgctgttc
    41941 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    42001 gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa aatcacgcag atattttacc
    42061 cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg ggcacgaatc
    42121 gcttcacgca ggttacccat ccagttgatg ccggaataaa gcgccaccgc caggccgaca
    42181 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtagcggct
    42241 agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    42301 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    42361 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    42421 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    42481 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    42541 atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    42601 tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc caggtactct
    42661 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    42721 tgcggataaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa cacttcaata
    42781 agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    42841 ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat gccgccagac
    42901 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    42961 tgcggtgtat aacgcactat tgcgtgtgaa gtgagatcgt cggggttttg cggataacca
    43021 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    43081 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    43141 agaatatcta ccggacggtc atggctactc agttccagct caattcccgg atgctgatag
    43201 agaaattccg acaggtgcgg cagtaacagg ctttttgcga ttcctggcgg gatgtcgatg
    43261 cgtaatttac cgctgatact ggtggcatcc tgctgaaaca gaccgtccag ttcgttgaga
    43321 ttactcaaca catcttttgc tcgttgataa taggtcatgc cttctggcgt gagtttgacc
    43381 cgtcgcgtgg tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    43441 cgcgaaacac ttccttttgg caaagcaaag aaatccgctg cgcgggaaaa actttccagc
    43501 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    43561 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttattttttc taacatctaa
    43621 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    43681 aatagctttc tgagaaaggt gaatcccctt aaacagagcg tatttttcag cgattccctt
    43741 taaagtgaat agaggacata ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    43801 attttggctt gtggtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    43861 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    43921 gtaactattt atatcggtaa ctattttcta cgcgccatct ctgtagaatg gccaccgcca
    43981 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    44041 cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    44101 ttcatggaat gaaaatcagt ttacagcagc gtattccagg agtgagtatt cagggggcca
    44161 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    44221 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcaatttc
    44281 ctgaggttaa ggtgttaatt acggcgacgg actgcaataa acggtggtta caggaagtta
    44341 tccattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    44401 cggtaaatag tgcggcgatg gggataatgt ttctgcctgg agactggcgg acgacgccgg
    44461 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    44521 tagcggcggg cgaatcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    44581 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    44641 caatgatgtt aaatatctcc tcctgaagag gataatttta cagccagcag gaagttagcg
    44701 gctggctgta aaaatattat ggttcgccgt acaaaccaat taaacggcgt accacaccgt
    44761 tagtccagcc aaacccatcc tgcaacggat actcgccacc gccgccttcg cggggaacac
    44821 catcggcaat atggtatttt tcgatcagtt tgtgctgttc cagataaaac tgattcaccg
    44881 tcttcagcca gctgcgcgcg atttcatcgc ccagaaggtc atcgccatac attttaaatc
    44941 cctgaattgc catccattgt aacggtgccc agccgttggg tttatcccac tgttcaccgg
    45001 tttcgtactc gcttgccaga atcccgccag gtgtcagtaa ccgactgcgc acggcgtttg
    45061 ccagacgatc ggcctgttca tggttcgcca taccgacata gagcggcaca atggcggcag
    45121 cggaaaacag cgccagttgt tcgcgtcgcc agtcgtaatc gcggtagatg ccgttttcat
    45181 catcccagag gtaacggttt accgcatcgc gacgggcact ggccttctgg cggaacagtg
    45241 cttctgtctc tttctcacct ttcagcgccg agatgttggc gatggcgctc tccagtttaa
    45301 acaggaaggc attcagatcg atggggatga actgggtggt acgaatgctc gccagacgac
    45361 cagtatcacg gagccaacgg gaagagtaat cccaaccgga ggccgccccc gcgcgtaaat
    45421 cgcggtacac ctcgttgggc gggcgaccag aatgtttcgc ggtttcaacg tcctcaagcc
    45481 aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg gttgagcagc gatccgtccg
    45541 gcatccgcac aacatggcga taggcctgat tagggatcaa cgattctgca ccgtccatcc
    45601 agaaggcata ttccattttc aggtggtcga gatagcggcg cgcaccgcgt acaccatctt
    45661 cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg cgagcggctc aaataatagg
    45721 tgcggttgcc gttggggatg tgaccgtagt tttcgatcat ccaggcgaag ttatcggcca
    45781 tgcatttcag caaatcttcc cgaccacttt ccgccagccc cagcatggtg aaataggaat
    45841 cccaatagta ggtttcgcta aaacggccgc ccgggacaat atatgactgc ggcagcgcca
    45901 gcagagaaga ccacggaatg tgatcctgtg gttcgcgggt tagcaccggc cacagctggt
    45961 cgatatgctc tttcagggaa ttttgcgggt ccgatacata ctcgctggag tagacctccg
    46021 gcagccagaa gtgattttca acaaacttgc gcaagtcaaa atcacgatga cggcgcactt
    46081 tacggtagcg gattaaaata tccagcgggt ccattttggg tgcgcagtcg ggaaaggttt
    46141 tactgtcggg gaaaattttc gccgactgaa catgctcgaa cagttcgaga tagcgatcgg
    46201 caggcgtcag cgcatcagag gcgggcagcc cttcaatcat ctcgggttcc ggttctgcct
    46261 cgatcatctc atccagtttt aattcatacg ggtccagttc atagcagaga tcgacctcga
    46321 tcatcagttc gtctggatta gggttttgaa ttttctgatt gagcataagg atccaacctc
    46381 cgaaattcgt cgctaattat cgggcttttc tttaagggta gtcattcgct ccgcctggtg
    46441 ggcgacaaag cgtgcggtgg atcacgttta ttaacctgtc aaaaaatata acgcactaat
    46501 atcaatggaa tttataaaaa tacagatttc gtgttgtttt tcattttcag aacattcaat
    46561 cctgctcttg ccgcaatcat gatatgccat tcctgatctg ggtaataggc acaggctatc
    46621 ttattgatag tttatattca tgtaattgat tgattcctat caaaaagcgt atctccctgt
    46681 tttttatgat ggctttgtct cgcagagacg tttgcagcat tctggaaaat tttctgggtc
    46741 acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat taccgcgatc
    46801 ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg gtaccacgat
    46861 aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa taataaggtt
    46921 ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc agaattaccc
    46981 gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa gcttggatat
    47041 aaatctttta acctcgaggc cgtgcgtgct gctctgctgg ctgataaacc cgtttcgcaa
    47101 agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccgcc aacgcgttat
    47161 accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat actggcctgg
    47221 attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga gcatcgcaat
    47281 gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt ggcgttgggc
    47341 tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg cgctcattgc
    47401 catgcgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt tggtggcgca
    47461 gttgggccga ttaacgcgcc gacggtattt aactcagtat ttaacgttga gcagttctgg
    47521 gatggtcgtg cggcaacatt gcaggatcag gctggtggac cgccgttgaa cccgattgaa
    47581 atggcgtcaa aatcctggga cgaaattatt gctaagctgg aaaaagatcc gcagcttaaa
    47641 gcgcagttcc tcgacgtcta tccgcaaggt ttcagtggcg aaaatattac tgatgccatt
    47701 gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg gttgcgtggg
    47761 gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa agataataaa
    47821 tgtgcaactt gtcatggtgg aattattctc ggcggacgtt ccttcgaacc gttggggctg
    47881 aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat gaatgtgact
    47941 aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt tgctttaacg
    48001 gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa actgatgttg
    48061 cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt agctttcctg
    48121 cacagtctga acggggttta cacgccgtat atgcaggata aacaataatt aatttgatcg
    48181 cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt ttaatattat
    48241 catattaaat gtttatatta taaaagatca tttttctgct taggattttg ttatttaaac
    48301 acgagtcctt tgcacttgct tactttatcg ataaatccta cttttttaat gcgatccaat
    48361 cattttaagg agtttaaaat ggataagaag caagtaacgg atttaaggtc ggaactactc
    48421 gattcacgtt ttggtgcgaa gtctatttcc actatcgcag aatcaaaacg ttttccgctg
    48481 cacgaaatgc gcgacgatgt cgcatttcag attatcaatg acgaattata tcttgatggc
    48541 aacgctcgtc agaacctggc cactttctgc cagacctggg acgacgaaaa cgtccacaag
    48601 ttgatggatt tatccattaa caaaaactgg atcgacaaag aagaatatcc gcaatctgca
    48661 gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc tgcgccgaaa
    48721 aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat gctcggcggg
    48781 atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc aacggataaa
    48841 ccaaacctgg tgtgcggccc ggtgcaaatc tgctggcata aattcgcccg ctactgggat
    48901 gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggatcc gaaacgcatg
    48961 attgaagcct gcgacgaaaa taccatcggc gtggtgccga ctttcggcgt gacctacacc
    49021 ggtaactatg agttcccgca accgctgcac gatgcgctgg ataaattcca ggccgacacc
    49081 ggtatcgaca tcgacatgca catcgacgcc gccagtggtg gcttcctggc accgttcgtc
    49141 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
    49201 cacaaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga tgaagaagcg
    49261 ctgccgcagg aactggtgtt caacgttgac tacctcggtg gtcagattgg tactttcgcc
    49321 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
    49381 ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
    49441 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta ccggtcgccc ggacgaaggc
    49501 attccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtatgac
    49561 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
    49621 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
    49681 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagtgatca cccgaaactg
    49741 cagggtattg cccaacagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
    49801 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
    49861 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
    49921 tttaggggga taatatggct acattagtac agacaggtaa agctaagcag ctcacattac
    49981 tcggcttttt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
    50041 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
    50101 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
    50161 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
    50221 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
    50281 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
    50341 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
    50401 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatt gcattagcgg
    50461 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
    50521 ctgacttctc taaagtgggc accctggttg tatttgttgc cttcattttg agttatatgg
    50581 gcgtagaagc ttccgcaacc cacgtcaatg aaatgagtaa cccagggcgc gactatccac
    50641 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttgtcta
    50701 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta
    50761 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
    50821 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
    50881 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
    50941 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
    51001 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
    51061 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
    51121 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ctggtcgtgg
    51181 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
    51241 acatccaggg tgactctacc gatatgtatg ttgaattact ggtcgttagt ttcctggtgg
    51301 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
    51361 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
    51421 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ctatgtctga
    51481 atggttataa gataagggag cgataattca tcgctccctt tttcgtgctt gctgtcgtat
    51541 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa actagcacat
    51601 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actggttgca
    51661 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
    51721 gcgggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
    51781 ctggccacgg tgtctcgcct cgactggcca ccggtttcct cggttaacat tagtaacccc
    51841 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tttgcaacgt
    51901 ctcggtataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
    51961 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
    52021 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
    52081 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
    52141 ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg actggatcag aacgtctggc
    52201 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
    52261 gcagaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatact
    52321 gagtcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
    52381 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
    52441 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
    52501 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
    52561 tacgctgaca ctcgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
    52621 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
    52681 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
    52741 ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggagct gaaaaagcag
    52801 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
    52861 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
    52921 actcccattc ttactttgta acaagccggt gctttaccca ctggcttgtt accctcttga
    52981 actatcccac cagcaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacgctt
    53041 agttaaacag ccataaatcc cctaataaaa atgtatgaat atgaaaaacc atacgatatt
    53101 cgcagtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat
    53161 tgaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg
    53221 gtgcagttag tcgatctcgc acgtcgcgtt tcattttgat tccgtctgat ttcccagcct
    53281 tataagggtg tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact
    53341 tgtcgagcag gcagatccgc ccattcgtgc taaagccgcg gaaattgccg ttgcgcatgc
    53401 tcattatctg agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt
    53461 cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa
    53521 cgtgctttct gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc
    53581 ggaagtgcat gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct
    53641 gaaaaagatc ctctatccgg taatcttctc ttcggattat tctgccgcag aaaaacttca
    53701 ggtctaccat ttctcgatta acagtattga tatcgctatg gaagtgatga cccgcgcgtt
    53761 tacctttagt gacagtagtg cctcaaaaga agatgaaaac tatcgtatct tctcgttact
    53821 ggaaaacgcc gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga
    53881 tattatctat aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc
    53941 tgattttggc ctgtggttta accataaagg tcgacattat ttcagcggca ttgctgaagt
    54001 gggccatatc tctcgtctga ttcaggattt cgacggtatt ttcaatcaaa ccatgcgtaa
    54061 caccaggaat ttgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac
    54121 cgtatcgcaa attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt
    54181 cggtatggat gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg
    54241 cgaaattgcc catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga
    54301 taaattcaaa gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa
    54361 agtctctcag gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg
    54421 cgatgaattt atcattgttt tgactgaagc ttctgaaaac gaaacgttac gtaccgcaga
    54481 acgtattcgc agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aagatattgc
    54541 cctctcactt tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat
    54601 tcaaatagcc gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact
    54661 ctggaaagcc agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc
    54721 ggataatgcc gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt
    54781 gttaattaac gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg
    54841 gtacaaacgt gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg
    54901 tcctgcgcat cctgaataca ttcgtcacaa ccgtgaaggc ggtaaagcgc gcgtcgaggg
    54961 gatgagtcgg gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc
    55021 gctatcgaaa gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag
    55081 cgtagaaatg gcgcaaaaag aacagaccag gcaattgatt attgccgttg accatctcga
    55141 ccgaccggtg attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac
    55201 cgaaatgttt ggttactgca ttagcgaagc cagcggtatg cagcctgata cactcctgaa
    55261 cattcctgaa ttccctgccg ataaccgcat tcgtttacaa cagttgcttt ggaaaaccgc
    55321 ccgcgatcag gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc
    55381 ttctatcagc ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgaccttctc
    55441 ggatatcacc gaagaacggc aaatccgcca gcttgaaggc aatattctcg ccgccatgtg
    55501 cagcagcccg ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgttct
    55561 caacgaatcg catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc
    55621 atcttcgcac ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg
    55681 tgatggcgcg cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc
    55741 ctttatcgaa cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga
    55801 aaaaagccgt cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa
    55861 tcgcaataac ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccagtatc
    55921 ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca
    55981 ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt
    56041 atcgaaggta cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa cattacccag
    56101 atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga taaccccttc
    56161 ccgcttacct tgagtattgg catcagctac gacgtgggta aaaatcgcga ttacctgctc
    56221 tccactgccc ataacgcgat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc
    56281 ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa
    56341 gaagcgatta gcaataacca actgaaactg gtttaccagc cgcaaatctt cgcagaaacg
    56401 ggtgaactct acggcatcga agcccttgct cgctggcacg atccccagca tggtcatgtg
    56461 cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc
    56521 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
    56581 ccggcgttat ccgtgaactt gtcggcgctg cactttcgca gtaatcagct gcctaatcag
    56641 gtgtctgatg caatgcaagc ctggggtatt gacggccacc agctgacggt agaaatcacg
    56701 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat
    56761 atgggcgtgg gtttatcggt agatgatttt ggcacgggct tttccggatt atcccgctta
    56821 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacg
    56881 gaaaaacgca tcctggcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
    56941 accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg aaagatccac
    57001 tgtcgcgtta ttcagggata tttcttttcc cgccccctac ccgccgaaga aattccaggc
    57061 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctctcccg cactcgcggg
    57121 ttttcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga
    57181 ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
    57241 aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga
    57301 tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata
    57361 ccaccgaact ggttgattta gccgtgatct tccctgatct ggagatcgaa ttgaaatacg
    57421 cctgcgctga taacatcaca ggtaaagcta tttaccagca agcgcgttgt ctgttacaca
    57481 aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctatca ggcttacaac
    57541 tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc
    57601 cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga
    57661 tcgacctgac gcttcgtgat gagcacggga acatcctgga tatgggcgca gggttcgatg
    57721 aaatgcacga gcgctcccat gcctatcacc cttccgtccc gcccgccgct cagcgcaatc
    57781 gcctgttgct gaatgcgata atgactggtg gcggttttgt cggtatctcc agcgaatggt
    57841 ggcacttcga attaccccag gcagcgagtt accctctgct cgctgatcaa ttcacctgtt
    57901 ttatatcccc tggcacacag cacgtcagtt aatcaggaaa ctgccatgaa gagatcgata
    57961 tcgtttcgtc ccacattgct cgcgctcgtc cttgccacaa ctttcccggt tgcgcacgcc
    58021 gccgtaccga aagatatgct ggtgattggt aaggccgccg atccacaaac cctcgacccg
    58081 gcggtaacaa tagataataa cgactggaca gtgacctacc cgtcttatca gcgactggtt
    58141 cagtacaaaa cggacggtga taaaggctca accgacgttg aaggcgatct ggcaagtagc
    58201 tggaaagcgt ctaacgatca aaaagagtgg acgttcaccc tgaaagataa tgctaaattt
    58261 gccgatggca cacctgtcac tgccgaagca gtaaaactct cttttgagcg gttactaaaa
    58321 atcggccagg ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa
    58381 catacggtga agtttaccct tagccagcca tttgcaccgt tcctctacac gctggcgaat
    58441 gacggtgcat ccattatcaa tccggcggta ttaaaggaac atgcggcgga tgatgcccgc
    58501 ggcttcctcg cgcaaaatac cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa
    58561 ggtcagcaat tagttctggt gccaaatccg cattaccccg gcaataaacc gaactttaag
    58621 cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc
    58681 gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaac
    58741 aaagtcaatg tggcagagta tccgtcactg cgcgtcacct atctgtatct gaataacagc
    58801 aaagcgccac ttaatcaggc ggatctgcgg cgggccattt cctggtctac cgattatcag
    58861 ggcatggtta acggcattct gagtggtaac ggaaaacaaa tgcgcggccc gattccggaa
    58921 ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa
    58981 gccgaatggg ataaagtgac gagcaaaccc accagcctga cgtttctcta ctccgataac
    59041 gatccgaact gggaaccgat cgctctggca acacaatcca gcctcaacaa gctgggcatc
    59101 aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat
    59161 tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat
    59221 tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt
    59281 gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccga
    59341 gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag
    59401 aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg
    59461 gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg
    59521 ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat
    59581 ctcgcatctg atccccggcg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc
    59641 tatcgtggaa aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta
    59701 ccgctacgtc agcgacctgt ttcatggtga cctgggaaca tccattcgta ctgggcgtcc
    59761 ggtgctggaa gagttgcgta tatttttccc ggcgacgctg gagctggctt tttgtgccct
    59821 gctgctggca ctcttgattg gcatcccgct gggcatactc tctgcagtct ggcgaaatcg
    59881 ctggctggat catctggtgc gaataatggc gattaccgga atctccacac ctgcgttctg
    59941 gcttgggctg ggcgtcatcg tgctgtttta tggtcatctg caaatacttc ccggcggcgg
    60001 aaggcttgat gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc
    60061 gttgcttgaa ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc
    60121 attaacgctg gcgtttgttc acctggggat tgtcgcccgc cagatccgct cagcgatgct
    60181 ggaacaattg agtgaagact acattcgtac cgcccgggcc agcggcttgc ccggctggta
    60241 tatcgttcta tgttatgcgc tacccaatgc gttgatccct tcgattaccg tattggggct
    60301 ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg
    60361 aatgggggca tgggtagtga catcaataca ggcgctcgac ttcccggcag tgatgggctt
    60421 tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt
    60481 gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc
    60541 gccgtacgcc cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa
    60601 ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgctact gatgatgatt
    60661 ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg
    60721 ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc
    60781 gtactggtcg gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt
    60841 atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc
    60901 atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg
    60961 gctgccgctc tcgggccgag tttgtttaac gccatgctgg ctattgctat tgtgcgaatt
    61021 cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt
    61081 caggcggcga aaacctttgg cgcgtctcgt tggcatctga tcaactggca tattttacgt
    61141 aactccctac cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg
    61201 gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg
    61261 atggtagcaa atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg
    61321 gcagcgattt tgcttaccgc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg
    61381 cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacaac
    61441 tgcatttgag tttccccggt tttaacggcg acgttcacgc gctcaacaat gtgtccttgc
    61501 agattaaccg cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca
    61561 ccgcaatgct gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga
    61621 tttcactgct gggagacgat gttcttaacg cgcgggaaaa acagcttcgt cagtggcgcg
    61681 gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa
    61741 taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca
    61801 gagctaaagc gattgccctg ctggaagaga tgcaaatccc ggataccgtg gaagttatgt
    61861 cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat
    61921 tctcctgcga accgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg
    61981 tacagttgca ggtactgcgt ttgcttaaac ataaagctcg cgacagtgga actgcggtac
    62041 tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt
    62101 atgccggaag cgtgattgag agcggcgtga cggcagacgt tatccatcat ccccggcatc
    62161 cgtataccat tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagccattac
    62221 ccgccattcc agggacggta ccaaatctca cccatttgcc tgacggctgc gcttttcgcg
    62281 atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg
    62341 acaacaacca gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt gtctgacacg
    62401 ttattaacgt tacgcgacgt ccatatcaat ttcccggcgc gtaaaaactg gcttggtaaa
    62461 actacggaac atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc
    62521 ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt
    62581 atgctgcaac cgagccacgg gcagtacatc cgttctggct cacaacgcat tatgcagatg
    62641 atgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg catcatcaca
    62701 gaaccgctct ggatagctaa gcatagtagt gaacaacagc ggcgagcgtt ggcagaggag
    62761 ctggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc
    62821 ggcgggcagc ggcaacgcat cgccattgcc agagcactct cttcgcagcc tgacgtgatt
    62881 gtgcttgatg agccaacctc tgcgctggat atctccgtgc aggcgcagat cctcaattta
    62941 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    63001 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtggaatta
    63061 ggggaagcgc aacaggtgct aacggctccg gcacatccat acacccgatt attgctggat
    63121 tccctccccg ccattgataa accactggag gaagaatggg cattacgtaa aacggatctg
    63181 ccaggaaacc gcacgttgcc acagggctgt tttttccgcg aacgttgccc ccttgcaacc
    63241 agcggatgcg aagtccggca atcattaacg acgcgggctg acggacgtga gatccgttgc
    63301 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt catgcgcctg acgcgtcatc
    63361 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    63421 agaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    63481 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt
    63541 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    63601 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    63661 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    63721 cgccactctc ggtggatact gttcccttcc cgcgtttgat atcgctttcc cagtgtgcct
    63781 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    63841 tacgtgagaa accgcacgag gataagcagg atattccgaa taaaatacgg caaaaatatt
    63901 acttctgcac ttaaattaaa ttcccacaat agataaccgg aaatatatat ctcaagagag
    63961 attgcatcaa tattaatcaa ctcatcacaa taataatacc tatcaatatc atggggttaa
    64021 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    64081 tgaatgtgct ttataaatct gcgatccgta gcagacacta taaatacaca gacacggaga
    64141 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    64201 gccattgagc aaggccttcg ggcagagcac ggtgttgtca ctgaagatga cattctcatg
    64261 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    64321 acgatcaatt atgtggtcag tggccagcac cctacgcttt aagatgctat gcttgatcgg
    64381 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    64441 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    64501 tggaccataa aatatctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    64561 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    64621 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    64681 tggggccgac gccctggcgg taaagcaaag acgataaaag ccccccaggg atggatattc
    64741 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    64801 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    64861 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    64921 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    64981 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    65041 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    65101 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    65161 tggacgatat tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg
    65221 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    65281 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    65341 tggatgtcgg aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta
    65401 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    65461 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    65521 cgttacttaa ccgctatcgc aatgaaattt gttcctttaa cgatgacatt cagggcaccg
    65581 cggcggtaac agtcggcacc ctgatcgcag caagccgcgc ggcaggtggt cagttaagcg
    65641 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    65701 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    65761 tcgatcgctt tggcctgctg accgacaaga tgccgaacct gctgcctttc cagaccaaac
    65821 tggtgcagaa gcgcgaaaac ctcagtgaca gggataccga cagcgatgtg ctgtcactgc
    65881 tggatgtggt gcgcaatgta aaaccagata ttctgatcgg cgtctcagga cagaccgggc
    65941 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    66001 cgctgtccaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggactg
    66061 aaggtaacgc gctggtcgcc acgggcagtc cgtttaatcc agtggtatgg aaagataaaa
    66121 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggg ctgggtgtta
    66181 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    66241 cccagtattc gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctaaaagata
    66301 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    66361 tggcggtgaa aacgtctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag
    66421 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    66481 cgggctattt ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    66541 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    66601 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    66661 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    66721 aatggcgatt ttggtgacaa aaccggcgta attctgggcc atgaagggat tggtgtggtg
    66781 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    66841 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    66901 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
    66961 gccgattacg cggtaaaagt gccagatggt ctggactcgg cggcggccag cagcattacc
    67021 tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa aaattcgtcc cgggcagtgg
    67081 attgctatct acggtcttgg cggtctgggt aacctcgccc tgcaatacgc gaagaatgtc
    67141 tttaacgcga aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
    67201 atgggtgcag atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag
    67261 aaaaccggtg gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg
    67321 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct
    67381 atgagcctgg atatcccacg tcttgtgctg gatggcattg aggtggtcgg ttcgctggtc
    67441 ggcacgcgcc aggatctaac tgaagccttc cagtttgccg ccgaaggtaa agtggtgccg
    67501 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc
    67561 aaaatccgtg gccgtatggt gattgatttc cgccgctaag aggcctttgc tgcgactccc
    67621 atgttcgggt cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg
    67681 cgtaggaagg aacaataatg atagtgttcc cagagaagga acgctactga gagtaaagac
    67741 actaatgaaa atggccaatc atccccgccc gggggacatt attcaggaat cactggacga
    67801 acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag
    67861 ccgattgctg accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt
    67921 gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc
    67981 tgcaaaaacg gtggatgtgt cgcgattacg ccgtctggtt gcgcaataaa aaatggcgcc
    68041 ttttacagcg ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat
    68101 caggcctaca cgttcactgc aatctattgg atttgaacgg tttgtaggcc ggataaggcg
    68161 ttcacgccgc atccggcatg aacaaagcct acgttgtcta caatatgaaa atggcgcctt
    68221 ttacagcgcc attttcagct taaagttcaa aagagattat atcccttctt cactctcttt
    68281 tttcgcttct gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg
    68341 acggctcacc ttcccttcga tcatcccttt aatcgatcct ttcacccaga atgccatata
    68401 catatggatc aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct
    68461 gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc
    68521 ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc
    68581 gttgtactta ccgacatccg ccactttatg ctcattgcct ttcaatactt cgacaatgtt
    68641 taacagccac ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat
    68701 aaacatcagt gcgacgaaaa tcgcaatgcc gaagaatggg tgcaaaatgc gtcccatctg
    68761 cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga
    68821 cagcgccacc aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt
    68881 ggtgcgcaca atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct
    68941 tattcgggcc aataccgatg tagtggaaaa tcaaccctgc aaaagtggca ataaagccag
    69001 ccgctgccag cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat
    69061 ctttcggcag accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt
    69121 gcgtaccacc gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca
    69181 gtttcgccac gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag
    69241 ccccggtcgg acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc
    69301 agagcgtgca tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac
    69361 acccggcaat gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt
    69421 tagcgtactg aatgattgca ccggcagacg ggcacgcctt caggcagccc ggatcttcac
    69481 agtgcataca gccatcttta cggatcagcc actccagctt gccgctctgt tcggtttcgc
    69541 taaagcgcat caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga
    69601 cgcagtgccc cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt
    69661 tacagccgat acaggtggaa acgtcgataa gttttgcgac ttctgctttg taatcacgca
    69721 cctgagaagg cggcgtgatg gagttagttg cggacctttt gataatgtcc tgcgtttcca
    69781 tagccatctg ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg
    69841 gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt gttagctata taacctttac
    69901 gcgcgacacc ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccat
    69961 ttacattcag cggtttcaga cgacgcgtta ccacagccac cgcgcggata aagccacgct
    70021 tgcttgagac agtgacacga tcgccattat taatgccttt cgccgccgcc agcgtttcgc
    70081 tgatttccac aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg
    70141 tgtggaagtg ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac
    70201 ccatccgcag cgcgtcttgt tcatacagac gaaccaccgg gttagaaacc acgttcggat
    70261 gcagcgggtt agttcccagc ggcgtttcaa ttggctcgta gtgttccggg aacggacctt
    70321 ccgccatttt gttgatggca aacaggcgtc ccatcccttc cggctgcatg ataaacggcc
    70381 cggttggcgt acccggtgcg gcattgccga agtcagggat atcgttaccc gtccacttgc
    70441 tgccgttcca ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatctgccg
    70501 aagcacggtt gtagagtacg cgacggttaa gcggccacgc ccaggcccat cccagcgtat
    70561 tccccagacc ggacgggtcg gagttatcgc ggttagccat ctggttgccc tgctctgtcc
    70621 agctaccggt gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa
    70681 agctactcag caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt
    70741 ccagcgcata gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct
    70801 tgtagttcca gctcatcttc atcagcggtt ctacgccttt accaccttca gcctggtaca
    70861 gctcgcgcag atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg
    70921 gcgcgtcctg acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat
    70981 cttcttctgc aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat
    71041 cgacatcgtt cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat
    71101 cgataaccac catgtacttc agcttgctca ggcagctcac cactttgttt ttgtccggga
    71161 aggacgcaac cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca
    71221 tgttgaaata cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc
    71281 agttgttctc tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact
    71341 tcggatagtt gctccagtag ttcacctgac cagccagcgt cgctttcggc gtgttcgctt
    71401 ccagatacga ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc
    71461 tggtagagag cagacctaag tcagtcagac cctgaatgtt ggagtgacca cgcaatgcat
    71521 tcacgccgcc accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac
    71581 ggatgttctg cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg
    71641 tgcgatccgg tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg
    71701 gcgtaccaca gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc tctttcagca
    71761 ggttccacac acagcgcgga tgagtcagtg tttcatcgcg ttttgcatag ccgttttcat
    71821 cgaactgata gttccaggac gacttatcgt actggcgttt ttcagcgtcg tagccgctga
    71881 acagaccgtc ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgct
    71941 taacgtattc ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga
    72001 acgtaatgtc cgtaccggaa cgaataggcg cgtagatatc cgccacagaa gcggtacgcg
    72061 taaaacgggg atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc
    72121 agcggaaacc gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag
    72181 cgtttttgat atccacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
    72241 ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg
    72301 agcgggcaaa tttctgggtc agcatcccgg tttcgttgct ggcaccggaa gcacacagca
    72361 taccggtaga aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag
    72421 cgtcacggtc agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc
    72481 gctgccattt gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcgctgt
    72541 tgacgtaatc cagcaaaccg gcccctttcg ggcacagcgc cccacggctt accggatgat
    72601 ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc
    72661 tatacatcaa tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag
    72721 cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg
    72781 ccgctactgt tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga
    72841 cgtccattgc tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat
    72901 tctgaatctt tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg
    72961 cgcaaattga cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta
    73021 aaaaaatgct ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat
    73081 gacacgacaa aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt
    73141 aggattgatt cgcggtgtca gtgaggggct cggcccggtc ggtggcgcag ctgctatcta
    73201 ttcattaagc gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcaaatccc
    73261 gaaaggctat ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct
    73321 ttccttaggg tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct
    73381 gtggcccagc ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct
    73441 gattgtacca ggattattat tagccctcgt cggcgtctgc tgggtgttag gcggtgacaa
    73501 tgggttacat tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct
    73561 ggcgttcatt ggtgcgttta tctgggcagc ctactgcaca gtaacgaata aatacgcacg
    73621 cggatttaac ggaattaccg tttttgtcct gctaacggga gcaagtctgt gggtttacta
    73681 ttttctcacg ccacaaccag aaatggtatt tagcacgcca gtcatgatta aactcatctc
    73741 tgcggcattt accttaggat ttgcttatgc tgcatggaat gtcggtatac tgcatggcaa
    73801 cgtcaccata atggcggtag gttcgtattt tacgcccgta ctttcctcag cgcttgcagc
    73861 cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa ggcgcgctga tggtctgcgg
    73921 cggttccctg ctctgctggc tggcgacacg tcgtggctaa aataattgcc gggtctacgg
    73981 cccggcaatt aatcagataa accccgacaa tattaaaaaa taaatttcaa ataccagtaa
    74041 ttcaccttat atctcatttc gactccgatc acatatcgat aaataatat
//
LOCUS       DAZFTN010000011        66378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFTN010000011 DAZFTN010000000
VERSION     DAZFTN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..654)
                     /locus_tag="ACHKV2_000872"
     CDS             complement(<1..654)
                     /locus_tag="ACHKV2_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO6938802.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     "
     gene            complement(723..1148)
                     /locus_tag="ACHKV2_000873"
     CDS             complement(723..1148)
                     /locus_tag="ACHKV2_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6938803.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1132..1455)
                     /locus_tag="ACHKV2_000874"
     CDS             complement(1132..1455)
                     /locus_tag="ACHKV2_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6938804.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            1580..2056
                     /gene="racR"
                     /locus_tag="ACHKV2_000875"
                     /gene_synonym="rarC"
     CDS             1580..2056
                     /gene="racR"
                     /locus_tag="ACHKV2_000875"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO6938805.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            2372..2524
                     /locus_tag="ACHKV2_000876"
     CDS             2372..2524
                     /locus_tag="ACHKV2_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6938806.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(2639..3154)
                     /locus_tag="ACHKV2_000877"
     CDS             complement(2639..3154)
                     /locus_tag="ACHKV2_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938807.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            3278..3676
                     /locus_tag="ACHKV2_000878"
                     /pseudo
     CDS             3278..3676
                     /locus_tag="ACHKV2_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            3738..4007
                     /locus_tag="ACHKV2_000879"
     CDS             3738..4007
                     /locus_tag="ACHKV2_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6938808.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            3976..5094
                     /locus_tag="ACHKV2_000880"
     CDS             3976..5094
                     /locus_tag="ACHKV2_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6938809.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            5261..6055
                     /gene="potC"
                     /locus_tag="ACHKV2_000881"
     CDS             5261..6055
                     /gene="potC"
                     /locus_tag="ACHKV2_000881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO6938810.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            6052..7098
                     /gene="potD"
                     /locus_tag="ACHKV2_000882"
     CDS             6052..7098
                     /gene="potD"
                     /locus_tag="ACHKV2_000882"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO6938811.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(7254..8075)
                     /gene="cobB"
                     /locus_tag="ACHKV2_000883"
     CDS             complement(7254..8075)
                     /gene="cobB"
                     /locus_tag="ACHKV2_000883"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO6938812.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(8091..9002)
                     /gene="nagK"
                     /locus_tag="ACHKV2_000884"
     CDS             complement(8091..9002)
                     /gene="nagK"
                     /locus_tag="ACHKV2_000884"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO6938813.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(9031..10275)
                     /gene="lolE"
                     /locus_tag="ACHKV2_000885"
     CDS             complement(9031..10275)
                     /gene="lolE"
                     /locus_tag="ACHKV2_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO6938814.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(10275..10976)
                     /gene="lolD"
                     /locus_tag="ACHKV2_000886"
     CDS             complement(10275..10976)
                     /gene="lolD"
                     /locus_tag="ACHKV2_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO6938815.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10969..12168)
                     /gene="lolC"
                     /locus_tag="ACHKV2_000887"
     CDS             complement(10969..12168)
                     /gene="lolC"
                     /locus_tag="ACHKV2_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO6938816.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            12430..13503
                     /gene="ycfT"
                     /locus_tag="ACHKV2_000888"
     CDS             12430..13503
                     /gene="ycfT"
                     /locus_tag="ACHKV2_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO6938817.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            13631..17077
                     /gene="mfd"
                     /locus_tag="ACHKV2_000889"
     CDS             13631..17077
                     /gene="mfd"
                     /locus_tag="ACHKV2_000889"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO6938818.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            17221..18183
                     /gene="ldtC"
                     /locus_tag="ACHKV2_000890"
     CDS             17221..18183
                     /gene="ldtC"
                     /locus_tag="ACHKV2_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO6938819.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(18266..18523)
                     /gene="bhsA"
                     /locus_tag="ACHKV2_000891"
                     /gene_synonym="ycfR"
     CDS             complement(18266..18523)
                     /gene="bhsA"
                     /locus_tag="ACHKV2_000891"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO6938820.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18764..19396
                     /gene="comR"
                     /locus_tag="ACHKV2_000892"
     CDS             18764..19396
                     /gene="comR"
                     /locus_tag="ACHKV2_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO6938821.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(19458..19997)
                     /gene="ycfJ"
                     /locus_tag="ACHKV2_000893"
     CDS             complement(19458..19997)
                     /gene="ycfJ"
                     /locus_tag="ACHKV2_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6938822.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(20224..21528)
                     /gene="ndh"
                     /locus_tag="ACHKV2_000894"
     CDS             complement(20224..21528)
                     /gene="ndh"
                     /locus_tag="ACHKV2_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO6938823.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(21928..22470)
                     /gene="ycfP"
                     /locus_tag="ACHKV2_000895"
     CDS             complement(21928..22470)
                     /gene="ycfP"
                     /locus_tag="ACHKV2_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO6938824.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(22493..23518)
                     /gene="nagZ"
                     /locus_tag="ACHKV2_000896"
     CDS             complement(22493..23518)
                     /gene="nagZ"
                     /locus_tag="ACHKV2_000896"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO6938825.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(23529..24353)
                     /gene="thiK"
                     /locus_tag="ACHKV2_000897"
     CDS             complement(23529..24353)
                     /gene="thiK"
                     /locus_tag="ACHKV2_000897"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO6938826.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(24334..24975)
                     /gene="lpoB"
                     /locus_tag="ACHKV2_000898"
     CDS             complement(24334..24975)
                     /gene="lpoB"
                     /locus_tag="ACHKV2_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO6938827.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(24989..25366)
                     /gene="ycfL"
                     /locus_tag="ACHKV2_000899"
     CDS             complement(24989..25366)
                     /gene="ycfL"
                     /locus_tag="ACHKV2_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO6938828.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(25369..25728)
                     /gene="hinT"
                     /locus_tag="ACHKV2_000900"
     CDS             complement(25369..25728)
                     /gene="hinT"
                     /locus_tag="ACHKV2_000900"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO6938829.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            25859..26041
                     /locus_tag="ACHKV2_000901"
     CDS             25859..26041
                     /locus_tag="ACHKV2_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938830.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAS
                     KIIRTLNITAHHPPPA"
     gene            26074..28263
                     /gene="fhuE"
                     /locus_tag="ACHKV2_000902"
     CDS             26074..28263
                     /gene="fhuE"
                     /locus_tag="ACHKV2_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO6938831.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(28323..29756)
                     /gene="ptsG"
                     /locus_tag="ACHKV2_000903"
     CDS             complement(28323..29756)
                     /gene="ptsG"
                     /locus_tag="ACHKV2_000903"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO6938832.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(30051..30848)
                     /gene="ycfH"
                     /locus_tag="ACHKV2_000904"
     CDS             complement(30051..30848)
                     /gene="ycfH"
                     /locus_tag="ACHKV2_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6938833.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(30859..31863)
                     /gene="holB"
                     /locus_tag="ACHKV2_000905"
     CDS             complement(30859..31863)
                     /gene="holB"
                     /locus_tag="ACHKV2_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO6938834.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(31860..32501)
                     /gene="tmk"
                     /locus_tag="ACHKV2_000906"
     CDS             complement(31860..32501)
                     /gene="tmk"
                     /locus_tag="ACHKV2_000906"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6938835.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(32491..33513)
                     /gene="yceG"
                     /locus_tag="ACHKV2_000907"
     CDS             complement(32491..33513)
                     /gene="yceG"
                     /locus_tag="ACHKV2_000907"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO6938836.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(33516..34325)
                     /gene="pabC"
                     /locus_tag="ACHKV2_000908"
     CDS             complement(33516..34325)
                     /gene="pabC"
                     /locus_tag="ACHKV2_000908"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO6938837.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(34445..35686)
                     /gene="fabF"
                     /locus_tag="ACHKV2_000909"
     CDS             complement(34445..35686)
                     /gene="fabF"
                     /locus_tag="ACHKV2_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO6938838.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(35774..36010)
                     /gene="acpP"
                     /locus_tag="ACHKV2_000910"
     CDS             complement(35774..36010)
                     /gene="acpP"
                     /locus_tag="ACHKV2_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6938839.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(36221..36955)
                     /gene="fabG"
                     /locus_tag="ACHKV2_000911"
     CDS             complement(36221..36955)
                     /gene="fabG"
                     /locus_tag="ACHKV2_000911"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO6938840.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(36968..37897)
                     /gene="fabD"
                     /locus_tag="ACHKV2_000912"
     CDS             complement(36968..37897)
                     /gene="fabD"
                     /locus_tag="ACHKV2_000912"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6938841.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(37913..38866)
                     /gene="fabH"
                     /locus_tag="ACHKV2_000913"
     CDS             complement(37913..38866)
                     /gene="fabH"
                     /locus_tag="ACHKV2_000913"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO6938842.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(38934..40004)
                     /gene="plsX"
                     /locus_tag="ACHKV2_000914"
     CDS             complement(38934..40004)
                     /gene="plsX"
                     /locus_tag="ACHKV2_000914"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO6938843.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(40085..40258)
                     /gene="rpmF"
                     /locus_tag="ACHKV2_000915"
     CDS             complement(40085..40258)
                     /gene="rpmF"
                     /locus_tag="ACHKV2_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO6938844.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(40310..40831)
                     /gene="yceD"
                     /locus_tag="ACHKV2_000916"
     CDS             complement(40310..40831)
                     /gene="yceD"
                     /locus_tag="ACHKV2_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO6938845.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            41030..41614
                     /gene="yceF"
                     /locus_tag="ACHKV2_000917"
     CDS             41030..41614
                     /gene="yceF"
                     /locus_tag="ACHKV2_000917"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO6938846.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(41715..42674)
                     /gene="rluC"
                     /locus_tag="ACHKV2_000918"
     CDS             complement(41715..42674)
                     /gene="rluC"
                     /locus_tag="ACHKV2_000918"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO6938847.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            43247..46432
                     /gene="rne"
                     /locus_tag="ACHKV2_000919"
     CDS             43247..46432
                     /gene="rne"
                     /locus_tag="ACHKV2_000919"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO6938848.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSTHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(46627..47580)
                     /gene="flgL"
                     /locus_tag="ACHKV2_000920"
     CDS             complement(46627..47580)
                     /gene="flgL"
                     /locus_tag="ACHKV2_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6938849.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(47592..49235)
                     /gene="flgK"
                     /locus_tag="ACHKV2_000921"
     CDS             complement(47592..49235)
                     /gene="flgK"
                     /locus_tag="ACHKV2_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6938850.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(49301..50242)
                     /gene="flgJ"
                     /locus_tag="ACHKV2_000922"
     CDS             complement(49301..50242)
                     /gene="flgJ"
                     /locus_tag="ACHKV2_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO6938851.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(50242..51339)
                     /gene="flgI"
                     /locus_tag="ACHKV2_000923"
     CDS             complement(50242..51339)
                     /gene="flgI"
                     /locus_tag="ACHKV2_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6938852.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(51351..52049)
                     /gene="flgH"
                     /locus_tag="ACHKV2_000924"
     CDS             complement(51351..52049)
                     /gene="flgH"
                     /locus_tag="ACHKV2_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6938853.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(52102..52884)
                     /gene="flgG"
                     /locus_tag="ACHKV2_000925"
     CDS             complement(52102..52884)
                     /gene="flgG"
                     /locus_tag="ACHKV2_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6938854.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(53022..53777)
                     /gene="flgF"
                     /locus_tag="ACHKV2_000926"
     CDS             complement(53022..53777)
                     /gene="flgF"
                     /locus_tag="ACHKV2_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO6938855.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(53797..55002)
                     /gene="flgE"
                     /locus_tag="ACHKV2_000927"
     CDS             complement(53797..55002)
                     /gene="flgE"
                     /locus_tag="ACHKV2_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6938856.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(55027..55722)
                     /gene="flgD"
                     /locus_tag="ACHKV2_000928"
     CDS             complement(55027..55722)
                     /gene="flgD"
                     /locus_tag="ACHKV2_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO6938857.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(55734..56138)
                     /gene="flgC"
                     /locus_tag="ACHKV2_000929"
     CDS             complement(55734..56138)
                     /gene="flgC"
                     /locus_tag="ACHKV2_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6938858.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(56142..56558)
                     /gene="flgB"
                     /locus_tag="ACHKV2_000930"
     CDS             complement(56142..56558)
                     /gene="flgB"
                     /locus_tag="ACHKV2_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO6938859.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            56713..57372
                     /gene="flgA"
                     /locus_tag="ACHKV2_000931"
     CDS             56713..57372
                     /gene="flgA"
                     /locus_tag="ACHKV2_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6938860.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            57448..57741
                     /gene="flgM"
                     /locus_tag="ACHKV2_000932"
     CDS             57448..57741
                     /gene="flgM"
                     /locus_tag="ACHKV2_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6938861.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            57746..58162
                     /gene="flgN"
                     /locus_tag="ACHKV2_000933"
     CDS             57746..58162
                     /gene="flgN"
                     /locus_tag="ACHKV2_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO6938862.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(58202..59737)
                     /gene="murJ"
                     /locus_tag="ACHKV2_000934"
     CDS             complement(58202..59737)
                     /gene="murJ"
                     /locus_tag="ACHKV2_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO6938863.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(59847..60770)
                     /gene="yceM"
                     /locus_tag="ACHKV2_000935"
     CDS             complement(59847..60770)
                     /gene="yceM"
                     /locus_tag="ACHKV2_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6938864.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(60772..61419)
                     /gene="yceH"
                     /locus_tag="ACHKV2_000936"
     CDS             complement(60772..61419)
                     /gene="yceH"
                     /locus_tag="ACHKV2_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO6938865.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(61430..62014)
                     /gene="rimJ"
                     /locus_tag="ACHKV2_000937"
     CDS             complement(61430..62014)
                     /gene="rimJ"
                     /locus_tag="ACHKV2_000937"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6938866.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            62250..63458
                     /gene="mdtH"
                     /locus_tag="ACHKV2_000938"
     CDS             62250..63458
                     /gene="mdtH"
                     /locus_tag="ACHKV2_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO6938867.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            63522..64169
                     /gene="grxB"
                     /locus_tag="ACHKV2_000939"
     CDS             63522..64169
                     /gene="grxB"
                     /locus_tag="ACHKV2_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO6938868.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            64303..64863
                     /gene="yceB"
                     /locus_tag="ACHKV2_000940"
     CDS             64303..64863
                     /gene="yceB"
                     /locus_tag="ACHKV2_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6938869.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            64969..66015
                     /gene="pyrC"
                     /locus_tag="ACHKV2_000941"
     CDS             64969..66015
                     /gene="pyrC"
                     /locus_tag="ACHKV2_000941"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO6938870.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPIAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            66089..66334
                     /gene="dinI"
                     /locus_tag="ACHKV2_000942"
     CDS             66089..66334
                     /gene="dinI"
                     /locus_tag="ACHKV2_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO6938871.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        16237 a        17706 c        16517 g        15918 t
ORIGIN      
        1 tgcagcccgc tgtcgaaaat ccagcgacgg ccgccagaca ccagtcatcg gaaatttccc
       61 gatcggttcg ctcaggccat ccgggtattc aggttctgct gtctgcaacg gcgcgccatt
      121 cgactcactg gctggagcac tctcgcgcat gcgcgcgtta tgtgtggggt ttaattcttt
      181 atctgtatct gtatctgtcg tgacttgtcg tgacatatgc gtgacgcgtc gtgactcatc
      241 gtgacaatca gtgttctgct cccgcagtct ttcccgctcc cgctgcgctc tcttgcgctc
      301 tgccggggat tttgcggttt gcgaaacatt accgttatcc tccttcatca cctggcgttt
      361 ttcccatccg gaaataagat cgccatccag aactcgcccc tgcattgcat gcaaaattga
      421 atcaatcacg tcttccgtca catcaagcgc acttgctaaa tcttccgtcg tgacatcaat
      481 gtgaccacgt agtgactcgc cgtgacattt cgtgacattt cgtgacgcac tcaccagaag
      541 gtggatatac accgccatca ctgttgcgat tggctgtcct gagaccctgg caatggttcg
      601 ccatttggga tcatttggca tgtcatgcca caatctgagc caggcattag ccatactcac
      661 ctcatctgat accgaacttt accctcgaac atccggaaga aatccggcat gaatattgtt
      721 ggtcaatgca cgacaacagc attaccaggc tgacccccac tgttagtcag ggtgccccag
      781 gcgatcgccg ctgcgacaaa atcatccaca tctttcacca gccgatccct ccgttcgacg
      841 atctcacggt aatattcaga gctgtgactg cgcatacggg ccaccagcag aggcggcatt
      901 gccttttcga tcgccggtaa cagagcctgc attttttcaa cagcatcagg ggtgtcttta
      961 tccagccaac ggaaaatttt ctgggtatta cgagccaggg cttccggatg gctgtcgtcg
     1021 tacagttccg ggaacgtcat ccccagttcg aaataagtcc gggctatttc agctgcagga
     1081 actttctcac catcaggata tgcccaggca ttcatcgcca tgcggatgtg ctcatgtttg
     1141 attttcatga atcatttgcc tcttgatgct tcgggtatga tcgttttcgt catttggttg
     1201 cttcatcgac atattctgcg aataacatga cgagcgtcgt aagtatgtcc aatcaacatc
     1261 aggacgaagt tcttcacaca ggacaccacc ttttgttgct cgttcaatcg caggacatct
     1321 ctcagcaggc aactgacgta cacctttgat ccattgattt acgcttggag gagatacacc
     1381 taaaagccta gccattgctg attgcccacc gacaacagca caagctcgtt tgaatgaata
     1441 gttatctttt ttcatcgaat gaactccaaa aaacacacaa taatattagg cttagcctaa
     1501 tgcaattgtc aataggctat gcctaacgct tcgagagtag gtattgccta atgcgatgcg
     1561 cataggagac tattaagcaa tgcttagtgg taaagactta ggccgagcga tagagcaggc
     1621 cattaacaaa aaaattgcat caggagccgt caaatcaaag gcggaaatcg cacgtcattt
     1681 caaagtccaa ccaccatcaa tccatgactg gattaagaaa ggttcgataa gtaaagacaa
     1741 acttccagaa ctatggcgtt tcttttctga tgtggttggt ccagagcatt gggggcttaa
     1801 cgaatacccc ataccaaccc catccacttc agatacaaaa agtgaacttt tagacataaa
     1861 cagcctttat caagccgcct ctgatgaaaa aagagcaatt gtggctttcc tcttatctgg
     1921 aaatgctacg gagcctagtt gggttgatca tgacgttcgt gcctacattg ccgcaatgga
     1981 aatgaaggta gctaactatc tgaaaaatca agaatcaaaa cggaaaagcc agaacatcac
     2041 caagacagga acttaaactt atatggtccg acgggaaatt cctagttccc gttagttaac
     2101 tcctactacc tctcccacaa accatcacct attaggtcgc acccaaatca ttaggcgtag
     2161 cctattgaca agcaattagg catttcctat agttttccca taccaaccca tcccgtccca
     2221 cacaatacag ggcaatacct agagttaccc ggcagtggtc agggattaag tagccagccc
     2281 gaggcgtaag aacatgacgg cagggttcaa ctttaactat gcagcaggtt tttgttccgc
     2341 taccccggcg ttaaggggat atgaggtcag catggatact cttgatcttg gcaacaacga
     2401 atctctggtg tgcggcgtgt ttcccaacca ggacggcacg ttcaccgcga tgacgtatac
     2461 caaaagcaaa acgtttaaaa ccgaatctgg cgcgcgtcgc tggttagcaa gaaactctga
     2521 ctaatgaaat ctggtaatta aggaatcctc cacgggggga gagtggtgca cacgcgccgg
     2581 acacaagcaa gcatccggca tgctctttaa caatctggat atccataaca gtaaaaatct
     2641 acagattgcc gttcagtttt ctggccaact cttcaataga tggaggtgat acgtaatccg
     2701 gatttttatt cataagaaac tgattttcac agtacaggca cctgctttta tgaaaaaatt
     2761 catattctct aaccgggaat ggctgaagta tcgatactat cttttgtcca aaacattttg
     2821 gacaaagatg catgattatg ctgccgccgt tcacggttac ctccttcgag tacacaaaag
     2881 ttcccgactc aagttggcta aggatataac cttccgtctg agcctcaaag ttttcaaatt
     2941 ctgcaatttt agctttgaga gaagcattta tttcttgata agtgcccacc agctcaacga
     3001 gagacacgca ttcgcgctga atagatgcaa gctttgagtt cagttcacca atagccgcat
     3061 ttacttcagc ttgagttttt gcctcgttca ttagttttgc aatctgggcg gtttcacgaa
     3121 tagccgtcat tgctgccgtt aattcagcga tcacattcaa tactcttatt gttgttgggg
     3181 atatccagat taacaagatc cttgttgttg gggaataacg gggaccacct cgcctgacgt
     3241 ggtaaaaagc aggcacacaa cacgaaagca cacggcgagg gacaccatta aatggtggct
     3301 taagcaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt tagatgatgc
     3361 actgttacaa ttgcgggaat ttatcgacga aaactccggt gaattttttg ttcaggtctg
     3421 gggaaatgga gccaacttcg acaacacgat tttgcgccgt tcatacgaac ggcaggggat
     3481 cccctgcccg tggcgttact acaacgatcg cgatgtacgc acaatcgttg agctggggaa
     3541 agccatagac ttcgatgcca gaacggctat tccattcgaa ggtgagcgcc ataatgcact
     3601 tgatgacgcc cgttaccagg caaaatacgt ttcagttatc tggcaaaaac tgatcccgag
     3661 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
     3721 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
     3781 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
     3841 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
     3901 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
     3961 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
     4021 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
     4081 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
     4141 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
     4201 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
     4261 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
     4321 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
     4381 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
     4441 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
     4501 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
     4561 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
     4621 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     4681 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     4741 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     4801 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     4861 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     4921 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     4981 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     5041 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     5101 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     5161 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     5221 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     5281 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     5341 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     5401 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     5461 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     5521 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     5581 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     5641 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     5701 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     5761 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     5821 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     5881 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     5941 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     6001 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     6061 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     6121 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     6181 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     6241 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     6301 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     6361 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     6421 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     6481 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     6541 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     6601 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     6661 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     6721 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     6781 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     6841 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     6901 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     6961 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     7021 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     7081 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     7141 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     7201 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     7261 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     7321 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     7381 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     7441 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     7501 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     7561 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     7621 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     7681 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     7741 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     7801 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     7861 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     7921 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     7981 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     8041 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     8101 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     8161 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     8221 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     8281 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     8341 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     8401 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     8461 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     8521 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     8581 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     8641 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     8701 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     8761 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     8821 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     8881 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     8941 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     9001 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     9061 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     9121 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     9181 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     9241 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     9301 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     9361 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     9421 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     9481 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     9541 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     9601 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     9661 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     9721 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     9781 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     9841 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     9901 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     9961 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
    10021 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
    10081 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
    10141 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
    10201 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
    10261 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
    10321 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
    10381 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
    10441 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
    10501 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
    10561 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
    10621 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
    10681 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
    10741 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
    10801 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
    10861 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
    10921 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
    10981 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
    11041 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
    11101 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
    11161 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
    11221 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
    11281 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
    11341 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
    11401 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
    11461 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
    11521 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
    11581 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
    11641 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
    11701 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
    11761 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
    11821 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
    11881 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
    11941 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
    12001 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
    12061 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
    12121 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
    12181 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
    12241 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
    12301 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
    12361 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
    12421 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
    12481 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
    12541 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
    12601 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
    12661 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
    12721 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
    12781 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
    12841 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
    12901 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
    12961 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
    13021 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
    13081 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
    13141 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
    13201 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
    13261 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
    13321 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
    13381 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
    13441 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
    13501 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
    13561 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
    13621 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    13681 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    13741 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    13801 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    13861 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    13921 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    13981 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    14041 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    14101 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    14161 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    14221 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    14281 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    14341 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    14401 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    14461 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    14521 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    14581 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    14641 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    14701 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    14761 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    14821 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    14881 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    14941 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    15001 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    15061 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    15121 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    15181 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    15241 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    15301 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    15361 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    15421 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    15481 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    15541 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    15601 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    15661 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    15721 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    15781 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    15841 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    15901 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    15961 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    16021 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    16081 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    16141 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    16201 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    16261 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    16321 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    16381 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    16441 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    16501 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    16561 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    16621 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    16681 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    16741 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    16801 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    16861 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    16921 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    16981 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    17041 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    17101 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    17161 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    17221 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    17281 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    17341 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    17401 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    17461 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    17521 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    17581 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    17641 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    17701 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    17761 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    17821 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    17881 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    17941 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    18001 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    18061 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    18121 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    18181 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    18241 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    18301 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    18361 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    18421 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    18481 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    18541 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgatag atgtgatctg
    18601 gatcacatac aagatatttt ttataacaat cattaattaa tttaaaaatc tttaaatttc
    18661 agttaaatag aaagcaatta tttttatggg atctggactg gtgaacggag gtgattattt
    18721 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
    18781 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
    18841 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
    18901 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
    18961 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
    19021 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
    19081 ctgctttacc ggcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
    19141 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
    19201 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
    19261 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
    19321 cgaaggtgca tcgttggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
    19381 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
    19441 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
    19501 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
    19561 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
    19621 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
    19681 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
    19741 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg atgttcatcc
    19801 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
    19861 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
    19921 ttcagactgg ccactgccgc tacgccaagc gcagctgcga caccaatccc gatacccgcc
    19981 aacattgatt tattcacggg aatacctcct tctttgcagt tatgccaatc atgcaagcga
    20041 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
    20101 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
    20161 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
    20221 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
    20281 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
    20341 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
    20401 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
    20461 atttttcagc agcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
    20521 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
    20581 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtca gttccaccac
    20641 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
    20701 gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
    20761 gccgccttca tcagcactgg tgaccatggt ttgcgtcaga acgcgaacgc caagtttcgt
    20821 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
    20881 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
    20941 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
    21001 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
    21061 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
    21121 ctctttgaca cctggtgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
    21181 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
    21241 aatagtgatt gttttcgcgt cacgatcaat atcaatgacg gaacccagct ggaactggaa
    21301 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
    21361 agtcgctact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
    21421 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
    21481 accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt
    21541 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
    21601 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
    21661 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
    21721 aaaaaacaga gtttattgtt tattatttga gcgaatttgt gagagtgata tagcgtgagg
    21781 ggtgtatgta gggcgttcgg taggtctgat aagaggcgca tcaggcattg atgctcgact
    21841 gtcggatgcg gcgtgaacgc cttatccgac ttacagaatt cggagcgcat caggtaccgg
    21901 caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
    21961 caaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
    22021 ataatgatgc aactcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa
    22081 aatcaccagg caacgatcgc gattcttctc gcggaagttg gtcacacact tagtggcaat
    22141 atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt
    22201 agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc
    22261 gcccagacca acgccgcaaa tcaggggacg ctcgtcgacg ttcagttgca acattttgtc
    22321 cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa
    22381 gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
    22441 agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac
    22501 ctgctttctc ttcctgccag cgttcatgta actgattcag acgggcgctg atcgctttcc
    22561 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
    22621 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
    22681 tgcagaccag gatcatatcg caacccgcat ccagtgatac ctgcccgcgt tcggcataac
    22741 tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
    22801 ccagttcctg acgcaaaacg gttttcagcc agtaggaaga accgctcgcc ggacgcggat
    22861 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
    22921 acgtgctgaa aaccgacata tctttggcac gaatttccgc ttgcgggcgt gggtcgcacg
    22981 gcgtctcttt gtgtgaatct gcagttactg caccgtgtcc tgggaagtgt ttcccggtcg
    23041 ttttcattcc ggcttcatgc atcccatcaa taaaccgact ggcgattgcc agggcttttt
    23101 ctgggtcggc atgataagaa cgctcgccaa tcgccgcgct gatatgtcct acatccagca
    23161 ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg
    23221 cctcttgcgc cagtttgccg ccctcttcca ttcccaacag cgcagcaaag gattgtgctg
    23281 ccggtaagcg ggtaaaacct tcgcgaaaac gctggacgcg tccaccttcc tgatctaccg
    23341 ccaccaccag atgattgcgc gatgctgcgc ggatctggcg caccagttca cgcaactggg
    23401 caggatcatg atagtttcgc gtaaagagaa tcagccctcc caccagcgga tgcgccagta
    23461 tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact ggacccacac
    23521 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
    23581 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
    23641 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
    23701 gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac cgccgccagt
    23761 tccagcgcga tatcaccatc tcctgcatac tcccagtcga tgagttttaa ccctgacgcg
    23821 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt
    23881 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
    23941 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
    24001 cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
    24061 gtttttacct cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc
    24121 ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
    24181 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
    24241 agaaaactac cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
    24301 aagcgcgaca gcaattcgtc gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc
    24361 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
    24421 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc
    24481 tatggcttta ctacgggtgc ctaaactatc ctgcggcgac aaacctaact gttgcttagc
    24541 catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca gcgcatttcg
    24601 cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc
    24661 aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg
    24721 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg cagtttgatc
    24781 ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac
    24841 aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
    24901 ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg
    24961 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
    25021 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atggtaacgc
    25081 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
    25141 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
    25201 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
    25261 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
    25321 gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
    25381 atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
    25441 ctcttgtccg ccatggcggt tggtgttcat gatcagacga tagccatctt cggcaatacc
    25501 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
    25561 agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
    25621 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
    25681 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
    25741 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
    25801 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
    25861 gctgccgtat atatctccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
    25921 tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat ttgcatttgc
    25981 atttgcaagc aaaattattc gcacactaaa tataaccgct catcaccctc ctccggcatg
    26041 agcctgacga caacataaac caagagattt caaatgcttt caacacaatt taacagggat
    26101 aatcaatatc aagccatcac caaaccgtca ctacttgccg gttgcatagc actggcacta
    26161 ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt tgagggttca
    26221 gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc
    26281 aaaatgcaga tgactcaacg tgatattcct cagtcggtca ctattgttag ccagcagcgg
    26341 atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct ggggatcagc
    26401 aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca gatcgataac
    26461 tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg cgacgcactt
    26521 tctgatatgg cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc
    26581 gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac cagtcgtgaa
    26641 tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta tgtggcggat
    26701 ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg cggctaccag
    26761 aataacgact catggctgga ccgctacaat agtgaaaaga ccttcttctc cggcattgtc
    26821 gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca gcgcattgat
    26881 gttaatagcc caacctgggg cggtttaccg cgctggaata ctgatggcag cagcaacagt
    26941 tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca acgataaaga gatcaacaag
    27001 gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact gaatgccacc
    27061 cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg
    27121 gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatta tgtcggcggc
    27181 accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt
    27241 tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag caaacaaaac
    27301 aatcgttact tcagttcatg ggccaacatc ttcccggatg aaattggcag tttctacaac
    27361 tttaatggca atttcccaca aaccgactgg tcaccacaga gcctggcgca ggacgatacc
    27421 acacatatga aatcgttata tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg
    27481 atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag catggagaaa
    27541 aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg gtcgacctac
    27601 gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg caaatatctg
    27661 gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat gaatagccgt
    27721 ctgaccacca cgttagccat cttccgtatt gagcaggata atgtcgctca gtccaccggt
    27781 acacctatcc ccggcagcaa cggcgaaacc gcctataaag cggtggatgg gacagtcagt
    27841 aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct gacatttggc
    27901 gcaacgcgct atattgcaga ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc
    27961 accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt gacagtcggc
    28021 ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta tggcaccttc
    28081 cgcgccgagc aaggtagcta cgcgttggtg gatctcttca cccgctacca ggtgacgaaa
    28141 aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga taccaacgtg
    28201 gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac gtatcaattc
    28261 tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca acgtcttacg
    28321 gattagtggt tacggatgta ctcatccatc tcggttttca ggttatcgga tttagtaccg
    28381 aaaatcgcct gaacaccaga accagcaacc actacgcccg ctgcgcccag tttcttcagg
    28441 ccagcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt aatacatgcg
    28501 tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg tgccatttcg
    28561 ctggtacctg tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt tttcagatcc
    28621 agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc gataccgacg
    28681 atcgggaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc gatcagaccg
    28741 tgcgaaaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa tgccaggcct
    28801 gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagga gaactcgatc
    28861 ggctcggtga taccggtcag gaacgaggtc agcgccgcgg agatcataat accgcccact
    28921 ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc tggcagaccg
    28981 tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc cgccatataa
    29041 cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc aatctgcatc
    29101 tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa
    29161 ccgtaaatgc caaacgcaac taccgggttc tggtaagcag cccactgaga gaaggtctgg
    29221 attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc
    29281 aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata ctcaggcagc
    29341 ttaatacggt agaaacggtt aaacatgtac gctgcgatcg caccggagat aatccctccg
    29401 agtacgccag tatccgccag gtgtttagag gcgatttctt cagcaggtaa atgcagtacc
    29461 agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac tgcagccagc
    29521 gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc
    29581 atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac ggcgggcagc
    29641 cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggcag tacggatacc
    29701 ggcagcatca gcgatttacc gaccttttgc aggttagcaa atgcattctt aaacataatt
    29761 gagagtgctc ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa ggggggagag
    29821 cctcgccgtg tacagggcat ctaagcgccc tttatttatt acacagagta aaataattca
    29881 gtgccaattt gtttgacggc tatcacgttt caacttcaga tcattggaga agttgcacat
    29941 tttgacaaaa catatgtcac aatatttcaa acgtggttat tcaccgccca attttcggcg
    30001 gtgaacttta ctcgttttag tcattaatta cgagctttaa aaaaactcat tcaacggatg
    30061 gattgaaggc gggaagcgtc gatgtgaaac agacgggcga agttatcggt ggttacctgc
    30121 gccagttctt caacggcaac acctttcaac acagccatgt attctgcaac gtcacgaacc
    30181 atcgcaggtt gattctcttt tcctcgatgc ggcaccggcg caaggtaagg tgagtcagtt
    30241 tccaccagta accgatccag ggggacataa cgcgcagcat cgcgcagttg ctccgcatta
    30301 cggaaggtca caatgccgga aaaggagatg taaaatccga gatccagtaa tttgcccgcc
    30361 gtttctctgt cctctgtaaa acagtgtagt acgccaccgc aatccgtcac tttttcttcg
    30421 cgcagaattg ccagcgtatc ggcgcgggcg tcacgggtat ggacgataac cggcttgttc
    30481 agttcacgac caatctggat atgatggatg aaggactctt gctgacgtac tttagtttcc
    30541 ggtgtgtaat aataatccag cccggtttca cccagcgcta caacaccctc ttctgccgcc
    30601 agacggcgta aatcttctac atcgtaggga tcattctggt tgagtggatg cacgccacaa
    30661 gaaaatacga cgttgtcacg ttcgcctacc agatcccgca tgtgtaaata acccggtaat
    30721 gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca
    30781 tccacgtcct tatgcaaaga ttcataatcc agaccatcga gatggcagtg tgagtcgact
    30841 aaaaacatga tgtctctctt aaagatgagg aaccggtagc acaacgcccg gttgcaggta
    30901 atgctcaata cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc ctgtgacaga
    30961 cattaactgt tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc gcgagggaga
    31021 aagatggttt gccagttcgg cgaccaggcc cggcacatca acattggtca cctgcgcagc
    31081 gccatgatgg cgttttagcg catccatcag caacgttgcc agccagtgta aacgcgccgg
    31141 agcttgttca tgattaaggg ccgccagcag cgaataccag tcgcccgatg gcacgctata
    31201 tgccaacgcc tgacacaatg tttcacgagc ctgccagtta tctccctgaa acaacgccag
    31261 tgccgcgcca ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat cctgtgacat
    31321 tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc ggcggcggcg caaggtaatg
    31381 taaccgacaa cgactacgta acgttgccag taaacgttca ggctcgcggg tagccaggaa
    31441 aaaccaggtt tctgctggtg gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc
    31501 ggtcagtaag gcagcatcgg ttacccagac aactttcgca ccacctaagc gtgcgtgctc
    31561 attcagcttt tcggtgacct cacgtaccgc atcaatgccc agcgtatttt tccctttttc
    31621 gggagccagg gtgtagtaat cgggatgcgt gccagcctgc atcaactgac atccacgaca
    31681 gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt aaataacggc tcagggcgta
    31741 gattaaagca tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct
    31801 tccggcctga tagctggcta ccagtttttc gaaatcaggt cgtaaccatg gataccatct
    31861 catgcgtcca actccttcac ccagcgggtc acggtagtgc ggattgcatc catcacggcc
    31921 tccagcggct gggtggcatc aatggtatga atacttttat cttgtgcagc cagttccaga
    31981 tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt gctcaatacg atccagctcg
    32041 ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa catcgagata gagcgttaag
    32101 tcggggcgaa aatccccgag aacagcatca cgcagtgttg ccagcatatg ttggtcaata
    32161 ccacgtcctc cgccctgata cgcctgagtg gagagatcgt ggcgatcgcc aataacccag
    32221 gtgccgttag ccagcgcagg tttgatgacc gtttctacca gttgaacgcg cgcggcataa
    32281 aacatcagaa cttcggcttt atcggtaatg atttcatcgc ctaccgattt gatatccagc
    32341 accaggcttc ttaacttttc ggcaagttgc gtaccaccag gttcccgagt gaaaaccatg
    32401 tcgcggatac ccagttgctc gagcgtctca accaccacat tacgcgcggt agttttgcct
    32461 gcgccttcca gcccctcaat gacgatatac ttactgcgca tttttttcct taagcacttt
    32521 cagataatcc tgcacagact tgttatgact ggcaagattg gtattaaacg tgtgaccacc
    32581 tttaccatcg gccacaaaat agagatacgg cgtttttgcc ggatgcgcag cagccttcag
    32641 cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt
    32701 atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg ccattataac gctctcccat
    32761 cccgtaaatc acggttgggt cggtctgcag gcgcatacca atgcgtaaac ggttgataaa
    32821 taccgaggca acctgatcgc gttcactggc aatggcggtt tctttttcga taattgatgc
    32881 catcgtcacc agctgatttt tatctttata aggcagaccg tccgcacgcc cttcccaggc
    32941 gctatcgacc gctttcacca ttttcttgtg cgctcgcttg agtaacgcga catcggtggt
    33001 attggcggta tacatccagg tgtctggcca gaaccaacct tcaatccact ccgggttttc
    33061 cagttcaagt gcctgcgcta cggtgatgta cttatcatcg ctcagcgtgt gcttgatata
    33121 cggggcctca cgcaattgct tgaggtagtc gctcagacgc atcccttcta ccagtcgcag
    33181 agggaactgt gcttctttac cgctttccaa caatttcagc atctcgcgca cggtcatctg
    33241 cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga tccggttcga tacgcagcaa
    33301 ccattgaaaa acccgtgggc gattgatgat cttatcggca taaagctgtt caccaagcgc
    33361 cagacgtccg gtccctggct tcagggtaaa tatcgtctct tctttgataa gcaatttgct
    33421 gtcggcaaga tagcgaacct tccagacgcc cacaccagcg gcgataccca gtaccaccag
    33481 caataacaag attatcaata acactttttt catgactaat tcgggcgctc acaaagtggg
    33541 gctaaatatt catataacgt tgctgacgaa aaggagacat cgccacaggc acgtacgggc
    33601 atcactggca ttaacgcatt acaaataacc atctcatctg cctgcaacgc ctcttccaga
    33661 gaggcttgca cttcgacaag ctgataagag gattgtgcca gcaaacggat acagaattgt
    33721 cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg tataaactac gttgcccttc
    33781 cgccagaaca aattagctgc acagcattcc gtaacccccc cttcgctgtc aaggaccagc
    33841 gcctcatcag cgtttgtctg ctcaagatga gagcggatca atacttgctc aagccgatta
    33901 agatgtttaa tacctgcaag atgaggattg cgccccagcc gcaccgggct tagcgccaac
    33961 gtgatccctt cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc
    34021 cgcgttgccg gtccactgtt caatgtgctg taccctcgcc cgccactacc gcgactgatc
    34081 acgactttca gtacaccatt ttgctgttct gctgccagcg ttttcatctc ttgttcaagc
    34141 tgaggccaga agtcacagga aatcatcaac cgctgacaag catcctgtag tcgctggata
    34201 tgcgccgata acaaactgac tttaccgtcg ataactctgg cggtggtaaa acaaccatca
    34261 ccaaactgcg ttgcccgatc gcttactgac agcgattcct gcttataacc gttaattaag
    34321 aacataccgg ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag
    34381 tcggaataaa agctaagaaa aaaggcccgc aagcggacct tttatatggg tggaaaatga
    34441 caacttagat ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac
    34501 acagcgtgta ttccattccg ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc
    34561 aaccttcatc cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca
    34621 ggatggagta gatagattct actgcacccg ccgcacctaa caggtgaccg gtcatagatt
    34681 tcgtggagct taccaacaca cggcttgcag cttcaccgaa gatggttttc accgcctgcg
    34741 cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac
    34801 tcgcttcaat gcctgcatca cgcagagcat ttgccattgc cagagctgcg cctgcgccat
    34861 tttctggcgg tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag
    34921 cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg
    34981 caccatcgcc cagtacgaaa ccatcacgct ctttatccca cgggcggctc gccgcttgcg
    35041 ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa accaccaaca cccagcggcg
    35101 tactggcttt ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac
    35161 gcgcagcatg gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg
    35221 ggccacgcag gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg
    35281 gaacgaagaa tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt
    35341 cgatcagtcc gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct
    35401 cttccgttat ttcaaggcca gaatcctgca tggcctgaac gccagcgaca attccatatt
    35461 gaatgaaggc atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat
    35521 cctttactaa gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc
    35581 tgatgccact ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag
    35641 gagacaacat gcccagtccg gtcacaacta cacgacgctt agacacgttt gtcctccagg
    35701 gagggaaaaa atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc
    35761 tggagatgtt cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg
    35821 tgattttctc agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta
    35881 ccagctcaac ggtgtcaaga gaatccgcgc ctaggtcttc aacgaaagaa gcattgttgg
    35941 taacttcttc ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga
    36001 tagtgctcat actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt
    36061 ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat
    36121 tttgaggcct taccccccaa taacgcaaat cattttgcac taattattac tcataaccac
    36181 gcagactgcg cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc
    36241 cgttcacatg caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa
    36301 ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc aggaacctgc accaggatac
    36361 ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa
    36421 caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga
    36481 tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca
    36541 cagaaccgat agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt
    36601 ttgacagacg gaaaacagat gaaaggttgg tttcgataat atcgttccac tcttcatctt
    36661 tcattcgcat taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt
    36721 caccaaattc tgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat
    36781 tcaacatcag acctttgccg ttagcaccta aataatcact gatcgcctga gcgccatttt
    36841 cactggtcgc agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc
    36901 ggccaattcc gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgatgt
    36961 tcctctttta aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg
    37021 tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac tttgcccggg ccgacttcat
    37081 agagatgttc tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt
    37141 tatacaactg acgtaccagt gcgtcacgaa tggcatcacc attggtttcg catttcacat
    37201 caacgttatt cacaacagga actgttggtg cgttaaaggt gattttcgct aattctactg
    37261 ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg
    37321 gcagagcgcg ttttgcgccc gcagctttac aggcagcgcc agcacgctca accgcttctt
    37381 tatgaccggc aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac
    37441 cttctgcagc ttcttcacac gcttttccaa tagacgcatc atccagaccg atgattgccg
    37501 ccatagcgcc cgttccttcc ggtacggctt cttgcatgaa cttgccgcgc atctcaacca
    37561 gacgcaccgc atcagcgaaa tcaatcacac cagcgcaaac cagcgcggag tattccccca
    37621 ggctgtgacc ggccatcatt gccggtgctt taccgccatg ctgctgccat acgcggtaca
    37681 gcgcaacaga ggcagtcaac agcgctggct gggtttgcca ggttttattc agttcttcag
    37741 ctggcccctg ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa
    37801 acgtttcttc gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag
    37861 aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac
    37921 gaaccagcgc ggagccccag gtgaatccac cgccaaaggc ttcaagcaga accaactgcc
    37981 ccggcttgat gcgcccgtcg cgtacagctt catccagcgc gcacgggaca gaggccgcag
    38041 aggtattacc gtggcgatcc agcgtcacca cgacattgtc catagacata ccgagttttt
    38101 tcgccgttgc actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag
    38161 aacggtcaag attattcgcc gccagcgtct catcaacgat gtgcgccagt tccgttaccg
    38221 caaccttgaa gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacgc
    38281 ggtcggcgtt tggcagcgtc agcaattcac cataactacc gtcggcatgc agatgggtgg
    38341 aaatgattcc cggctcttca gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa
    38401 taataatagt cccacgatct gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga
    38461 ccagagcata cttcactgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg
    38521 tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca acctttaatg cccaacatgc
    38581 tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga
    38641 tcaggccaat ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa
    38701 agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac
    38761 gagtgacaat ccactcgtca gaggtgtcca ccattttttc caaatcggcg tttgtccgca
    38821 cttgttcggg cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac
    38881 ttttcggtta tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc
    38941 agagttccgc ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg
    39001 cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg
    39061 accgcaaaag ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct
    39121 aacagacagg cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc
    39181 ttttgtagcc aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga
    39241 gatttcagca gagaaaggaa catcctgaca acaccttcca tcgtctttaa tgtgacattt
    39301 cctgtaaagc cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca
    39361 agatagccga tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata
    39421 ctatcgagac cctttacttc ttcttcacca atattgagca acgccacgcg aggattggga
    39481 atttccacca cctcttcagc caggactgag cccataatgg caaattgcac caacattgtg
    39541 ctgtcacaat cgacgttggc ccctaagtca aggaccaccg ttttgccctt ttgctgatgt
    39601 ggtaataccg tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt
    39661 gccagcccca tcagtgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct
    39721 ttcaccagct ccagggccac gcgcattgaa ctcccacgac tggcgcggat agcttgcgaa
    39781 ggccgggcat cactggcgat aactgactgc gcaggaataa tctgcagacg cgaacgttgt
    39841 tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga
    39901 gtgagttgcg aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaaggacca
    39961 aaatcccctc ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc
    40021 ggtttggtct ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc
    40081 gtgattactt agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac
    40141 gcaggtgttt ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt
    40201 catgggaacg acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg
    40261 accttactcc tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct
    40321 ttaagctggc taatacggca aatgggtttg gcttttgcgc ttcttcaggc agttcaccaa
    40381 agaccatgtc cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca
    40441 aggcgaggat gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa
    40501 cctcaatcgg ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac
    40561 taaaacaata cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga
    40621 gcgttaccgt caccttcgca tcgccgttta acactgcgag acgttggtta tcgatagcga
    40681 acgacatgga gcattccaca tcactgtcca cactgactac ggattcggcg acgcgctcaa
    40741 cctgatcagg agtatagata ccctggtaat caaggcgttt ttgagccgta cgaaccggat
    40801 cgagagtcag gggtaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca
    40861 tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg
    40921 ggttactgct ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc
    40981 atagtttaaa atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact
    41041 tattttagcc tccacctcgc cctggcgtcg cgctcttctg gaaaaactgc aaatctcttt
    41101 tgaatgtgca gcacccgagg tcgacgaaac tccacacagc gacgaatcac cgcgacagtt
    41161 ggtgcttcga ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt
    41221 aattattggt tcagaccagg tatgtgttct tgatggcgaa atcaccggta aaccgttaac
    41281 ggaagaaaat gcccgtctgc aattacgcaa agccagcggc aatatcgtca ctttctatac
    41341 tggactggcg ttgtttaatt cggcgaatgg gcatctgcaa acagaagtgg agccttttga
    41401 cgtccatttc cgtcacctga gcgaggcgga gattgataat tacgtgcgta aagagcatcc
    41461 cctgcactgc gcgggtagct ttaagagtga aggatttggc attactctgt ttgagcgttt
    41521 agaggggcgt gatcctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt
    41581 acgtcgggaa gggaaaaacc cgctgatggg atagtttttt gcgccggatg cggttacgtc
    41641 gcatccggca atcggtgctg gcggcctgat gcggcgtaaa cgccttatcc cattatcatg
    41701 cttatatgct cgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccatca
    41761 tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa cttcaacgct
    41821 gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat
    41881 tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ctgcatactg tgtatgcaca
    41941 cggatctgat gagtgcgccc tgttaccgga ctacaacgca ccagggtggc aaatgcatag
    42001 cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactcac acgcacgata
    42061 cgttcgccgc tttgcagaat atttttcaat aacggcgcct gaacgctctt cacatgcgac
    42121 tgccactgac cgcgcaccag cgccaggtaa tctttttgca tccctttttc acgtaattgc
    42181 tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc tgaggtgtcc
    42241 cggtcaagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct
    42301 tcaataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc
    42361 agcaccagga tgtgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc
    42421 agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg aatacgcacc
    42481 tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttcg
    42541 cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa
    42601 aagttgtcga tacgttgccc cgcttcgtcg gcggtgatag caacaatttt tacggatgga
    42661 gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc actcattttt
    42721 ctatgcttat atttagtttt gcaccttatt acttcactac gtgatcactt tattgatggt
    42781 tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta
    42841 aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc
    42901 cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag
    42961 caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta
    43021 ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct
    43081 gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc
    43141 gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact
    43201 ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt
    43261 aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta
    43321 tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa
    43381 aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca
    43441 cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtactca
    43501 tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga
    43561 taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag
    43621 ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc gtatcgaagg
    43681 cgacgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg
    43741 gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat gggatttaag
    43801 cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggccccgtt
    43861 cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact tacgtcagga
    43921 catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc
    43981 tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct
    44041 gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct
    44101 gccatccggt ggttcgattg ttatcgacag caccgaagcg ttaacggcca tcgacatcaa
    44161 ctccgcacgc gcgacccgcg gcggcgatat cgaagaaacc gcgtttaaca ctaacctcga
    44221 agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat
    44281 cgacttcatc gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga
    44341 agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct
    44401 ggaaatgtcc cgccagcgcc tgagcccatc actgggtgaa tccagtcatc acgtttgtcc
    44461 gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg
    44521 tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt
    44581 gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca ttgaaactcg
    44641 tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc cgcactacca
    44701 cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc tgccgaagct
    44761 gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtccgga
    44821 acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa cgccagctga
    44881 acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac cagcaactcc
    44941 tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg
    45001 tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac cggaacgtca
    45061 acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc gccgcgacac
    45121 ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca
    45181 agcacagcag cagactgccg agacgcgtga gggccgtcag caggctgagg taacggaaaa
    45241 agcgcgtacc gccgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga
    45301 tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca
    45361 ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct
    45421 caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa acggtagtcg caccggtagc
    45481 tgaagaaact gtcgctgccg aaccaattgt tcaggaagcg ccagctccac gcacagaact
    45541 ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa
    45601 tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct
    45661 gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccaatcgcc
    45721 aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg
    45781 ctatccgatt gtacgtccac aagatgtaca ggttgaagag cagcgcgaac aggaagaagt
    45841 gcaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac cggttgttag
    45901 cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc
    45961 gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca
    46021 gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagttgcag aacacgcaga
    46081 accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg aaactgctga
    46141 ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg tcgctgaagt
    46201 cgcagcagaa gttgaaacgg taaccgcggt taaaccagag atcaccgttg agcataacca
    46261 cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg caccgcgtca
    46321 cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac
    46381 ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt aataattagc
    46441 tcaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg aatcaacata
    46501 ttaaaattca tcgaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    46561 cgccccggcg atccctgagc gggcagacaa aacataccca gtttcatgat atgttttaaa
    46621 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    46681 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    46741 atcaaccaga tcgctcatct gctgcgtttg ccctaatgcg cgatcgctac ctaatgaatc
    46801 cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaacggtca ggacattgtt
    46861 cagcgagttt ttcagtccgc ggttggtttt atctaacgcc gcagcggcgg tttctttatc
    46921 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    46981 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    47041 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt
    47101 aatactttct gttccgccga cataatcacc gttaacctca ctaaacggcg cagtctctgt
    47161 tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa
    47221 ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcattgt cactcaaggt
    47281 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcggtgg tgacctggct
    47341 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg
    47401 gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc
    47461 tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc
    47521 ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    47581 tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    47641 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    47701 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    47761 gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    47821 taagcgtcgt taaaggattt cgctccgccc accgttttac tgttgctttg cagatccagc
    47881 agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgctatt
    47941 ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc
    48001 gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    48061 actttaccgt tggcatctgg cgtcaccgta aaagtggtat tgctggcaag gcgggtgacc
    48121 tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccgc ggaggcatca
    48181 gttaccgtgg caccgatcgc aacgttaccg ttgtttttag tgttttgcag aaccgcgggc
    48241 ttaccgatag caaagaaatc ttcaccctca tcgccgttgg catcaaatcc ggctttgtgt
    48301 tggctgttga aagcctcagc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    48361 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    48421 tccgggatct caatattgcc tgccgtccca tcaacataag cgacagtcgt acgagaaggg
    48481 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    48541 ccattggcca tcgtgatgtt ataagtgccg ccatcctgaa cgctgacttc tacaccaaca
    48601 atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt aggtgacgcc
    48661 cctgccccca cgcctgtcag gcgggagatt tgatcgttca ggctggcgat ttgtttagcg
    48721 taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt gtcctggtcg
    48781 cgaagatact gatcggtggt tttaaactga ttcaccaacc cttctgattt cccaatcagc
    48841 gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa
    48901 tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc gattttcgac
    48961 atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgccgcacg taactggttg
    49021 gtaataaacg catcatactc acgctgcaca ccagaaacgt agacaccatt accaacccag
    49081 ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg ggtatatccg
    49141 gcaacgttat agctggagat attattactt gccgtattta acgccgcctg ggccgcgttc
    49201 agtccgctca tggcgttatt gatcaagctg gacatggagg ttccttataa gccttcaatt
    49261 acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga ttatcaatgt
    49321 tcatgctgta ggttttgctc accttgtcgc ttatcgattt catctgctga atcatgttgg
    49381 tgagtttacg ggcatagtga ggatcggtgg catagcccgc gtcctgtagg gcctgcgccc
    49441 cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgt gttaacagcc
    49501 caacgtaatc cgacaatgct tccagatacg agctgtagac ccgaaacttc gcttttactt
    49561 tcttcgcttc gccattttca tattcagtcg tggtgatttc agtgactggc cctttccagt
    49621 tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg cggcggattt
    49681 gccgttgccc ccagccagat tccagcgccg cctgagcgag gatcaaatga tgtggcacac
    49741 cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat gctttactgt
    49801 cacctggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg
    49861 cctgattttg ataacgcacc acggtttcga gcgggaattt catcggtgct gctggcgtgg
    49921 actcctctgg caatggttgt tctggcgtca tctgcttaac catcatctct gcaagcccca
    49981 gacctttgcc cgtcgtcatc tgttgggcaa tctgctggtc atacatactg gtatacaggc
    50041 gagtgtgctc gctgctgaac aggccatctt ttggtaacgc gtcgcgcatg cttttcaaca
    50101 tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt gccgccggat
    50161 cttcgctcgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc gcacttgcca
    50221 gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac atcccgcact
    50281 ttgcattgat tgcaaaatag acatcagatc catcggcgta gcgcccagcg cattgagcgc
    50341 gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac cgccgctctg
    50401 gcgtaagtcg atctgcgttt gtggcgttac cacggtctgt ccgccaccaa acggtgtatc
    50461 tggttggctg acattggcct gacgattgac tgttactgag agatttccct gcgctatcgc
    50521 gcagctgtcg agagtcactt cgcgattcat caccaccgaa ccggtgcgcg agttaatcac
    50581 tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatcgg caaggaaacg
    50641 gacctgggaa ctgttgccac tcggtacgcg cacctgaata gtccgcgcat ccaacgcggt
    50701 ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa
    50761 atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc tgggtaattc
    50821 acgttcaata actgcaccat tggtgatccg tccaccgttc agttggttca cctgaacact
    50881 gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata
    50941 cacctgactg tcaacgccct taagcggagt catcaacaat gtgccgccac gcaggctttt
    51001 ggcatttccc atggaagaaa ccaccacgtc aatggtttgc ccctgacgtc caaacggtgg
    51061 aagtgacgcc gtcaccatta ccgcagcaac gttttttagc tgcatattgg tgcccgtcgg
    51121 aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg
    51181 ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa tcagtgagtt
    51241 ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt
    51301 aaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac ttacattggc
    51361 gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca
    51421 ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt attgctgccg
    51481 ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc ctgattaatg
    51541 gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc
    51601 accgtcaacg tgccgctaaa ggtattactg gcattggccc cgccttttcc gttgaacgtg
    51661 ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg caaatagcgc
    51721 ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc agaggagctt
    51781 ttgctggcgc taacgttctc ctgcaacacg atggtcagcg tatcgccaat attgcgtggt
    51841 cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga ctggaaaata
    51901 gaaccgttgg cgacgggcgt cggaccggga accggttgtg cactggtcgc cccctgcacc
    51961 agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg
    52021 gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca ataaaccggt
    52081 gaacctgcca ccggttatgc cttagagttg cgtcagtttt tgcagcatct gatcggtggt
    52141 ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc
    52201 ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt
    52261 caggcccggc gtgctttcgt tcggtgcacc ggaggattgc gtttcggtgt agaggttttc
    52321 gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct gcccaacctg
    52381 aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat
    52441 acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac
    52501 cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg atgagccatc
    52561 tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc tgttgttggt
    52621 ctgcgacagg ttgccttggc tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc
    52681 gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg gctggcgaat
    52741 ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac
    52801 gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt
    52861 ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattgctgac aagcagagca
    52921 gaagcacgcg gcggtgcgaa tttcccctca ccctaaccct ctccccaaag gggcgaggga
    52981 acagtccgct cgcttttatt gcaatgtcat caatcgttca attaactcat cgacagcagt
    53041 tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa
    53101 cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc
    53161 agaacccccg acatcacacg caaggttgga tctgcctgca gtaccggtcc acgcgtggcc
    53221 tgggtttctg cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg
    53281 gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc gcccggattc
    53341 agcgccgaga ttgttccatc ggcagctatc gtgatttccg ccccttccgg cacagcaatt
    53401 ggccccgcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt gggatcaacc
    53461 tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggtctgcac ggccaaccag
    53521 ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtt
    53581 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc
    53641 accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc
    53701 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcggta
    53761 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt
    53821 gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac gctgggcaac
    53881 gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg caccgttggt
    53941 cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    54001 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    54061 tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    54121 atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac
    54181 aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc
    54241 gggttctgcg ccgttaattg cgccggtggt aatattctct gttggatttg aggtcagaac
    54301 gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct
    54361 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc
    54421 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    54481 attgctggca tcaaatgcgt taacagaggg aagcggatcg ctggaattca ggttgatctg
    54541 catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatgttagt
    54601 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg
    54661 taaaccttgc atattcacca ggttacgatt ttcatccagc ttaaattgtc cgttacggct
    54721 gtagaacacc gaaccgttgc tatctaccag acggaaaaaa ccgttctggc tgatagcaac
    54781 gtccagacca cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    54841 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    54901 tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    54961 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    55021 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    55081 cagcgtatta ccgttgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    55141 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acgttgtaag aaccgttcgg
    55201 cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtgaactc cggcggtcag
    55261 ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac
    55321 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc
    55381 ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag
    55441 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt
    55501 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    55561 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa
    55621 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    55681 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    55741 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc
    55801 tgatagctgc gtgacgccga catggtgtta accatctctc cgacaacatc aacgttcggc
    55861 atttttacgt agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc
    55921 ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    55981 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    56041 ctatcagcat tcgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    56101 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    56161 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    56221 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    56281 ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccgga
    56341 atgtgttgcg ttgaggtcat cgtcagagca accacactgg ttgcattccg tccacgttgc
    56401 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    56461 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    56521 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    56581 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    56641 gtcgctattt atgccgttga tggtcattgt ggacaggtac aattcacgtt gtagaaatgg
    56701 ctgggggcga aaatgctggc aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    56761 ccgttaagtg cggcgagcaa tctcacatcg caattgcaaa ccttttttag ctcccagctc
    56821 gcagggataa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgccg
    56881 tgcgagcagc cattgctttc gatgagcaat aattcccgct tgtggggcaa tgtgaatgtg
    56941 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    57001 tatgtggttg ccgcgatgcc cattgtgcga ggaggaaagc tgggagctgg caatgtcaaa
    57061 ctgaaacgcg gacggctgga taccttgcca ccgcgtacgg tgctggatat caatcaactt
    57121 gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    57181 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag cggtgatggg
    57241 tttagcgcca acgcagaagg tcaggcgcta aacaatgcag ccgtcgcaca gaatgcgcgg
    57301 gtgcgcatgg tatctggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    57361 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    57421 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagccc
    57481 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacac ccgagcggca
    57541 aaaacaacag cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    57601 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    57661 aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag
    57721 caagacttgc agagtaatta atcgtatgac gcgtcttgca gagatcctcg accagatgtc
    57781 cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg
    57841 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcaac
    57901 gttggattac ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgttgatat
    57961 tagtcaacgc tggcaggaaa ttgctgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    58021 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    58081 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg
    58141 cagtaaaaag atttcgatct gaagattatc tccggcctgc actgcaggcc ggaatgcatt
    58201 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    58261 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagcaaacgc cagggcatgg
    58321 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    58381 gtaccgccac caccagacgc aacagaaacg ccatccagcc aggttgcggg gtaaagattt
    58441 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    58501 aaagtgacag cccggcatgt ttcaacggac cgataaacgc caggttcatc aattgcgtta
    58561 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    58621 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    58681 cccgctgggt catcagcgca tcaaaggcgg taaatttacc gtactggaac agcgaaacgg
    58741 ttaacggacc ggaaagaatg cccaacgcaa ccgcactcgg caacgccaac aggaaacaaa
    58801 gacgcagtcc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    58861 tcgacagcga cggtagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    58921 ccattaagcg gtcggcgtaa tacatccatg acaccgaacc ggaagcaaga aacgaggcaa
    58981 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatt gccggtccca
    59041 tctgtttcac cacacgcatt gctccggcat cgtggaagtt aatgcgtggc aaaaccagca
    59101 tgccgatctt cttcagatgc ggtagctgat acaccagctg caggacgccg ccgaccgtca
    59161 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    59221 caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg
    59281 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    59341 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    59401 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa
    59461 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    59521 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    59581 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    59641 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    59701 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    59761 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    59821 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    59881 acgatacgtt gcgccagcac tgcctgttcg ccggcggttt gcggaactgt ctgattttgc
    59941 acgcattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag cgtgctctgc
    60001 caaccaggaa tcggtttatt cactacaccc tgcccacgct cctcacgcca ttcgcgcatt
    60061 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    60121 gcacggcgat gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    60181 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    60241 cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    60301 agatcgtgtg gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg
    60361 gcgagttgcg tttttaactc acagtagagt ggtgcgaaac gacggttaaa accgaccatc
    60421 aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    60481 gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca
    60541 aagtgactgg cggtgctgga atgtacaaaa accgcatcgc aactggcggc aaggctggat
    60601 aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta
    60661 ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    60721 ttttgcgcaa tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc
    60781 caggtgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    60841 ttcgacgcgg gcctgtaaat caccgtcaac cgcgttcgac atatccgtta ccgccggctg
    60901 atcctctacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    60961 ggccagacgc accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcaactc
    61021 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg
    61081 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt
    61141 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    61201 accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    61261 agattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac
    61321 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc
    61381 aatcacacga gcttccaggg cagtaagttg atatttcatg agattctcct tagcggccgg
    61441 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    61501 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    61561 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    61621 tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc
    61681 caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg
    61741 caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt
    61801 gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga taacagtggc
    61861 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    61921 ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    61981 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    62041 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa
    62101 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    62161 taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    62221 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg aggaacctgg
    62281 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    62341 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    62401 ccctcggcct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    62461 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgtgcc gccggattcg
    62521 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    62581 tcggtggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    62641 aacagcgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    62701 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    62761 ccgttttgtt tgtgctgtgt gcagcgttca atgcgtggtt gttaccggca tggaaactct
    62821 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgcgac aagcgttttg
    62881 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    62941 tgccaattat ggtcaacgat gtggctggcg cgccctctgc cgttaaatgg atgtatgcca
    63001 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    63061 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    63121 tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    63181 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    63241 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata
    63301 tcggtggcgg ctggctgttt gacctgggca aatcggtgca ccagccagag cttccgtgga
    63361 tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag tttagccaga
    63421 aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc ccctgactcc
    63481 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    63541 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    63601 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    63661 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc
    63721 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    63781 aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca
    63841 aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    63901 aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata
    63961 tcagcgatga tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    64021 aactttcgga agatgatatt cagctattcc cgctactgcg taatctgacg ctggtagccg
    64081 gaattaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    64141 tcaatttgtt atcatcaatg gcgatttaat cctgactcgc cgcggcagcc tgtattcgcg
    64201 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    64261 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    64321 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    64381 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatt
    64441 ggtttacccg gtgtggctga cgcccatatt gttctgacaa acctgaccag ccaaattggt
    64501 cgcgaagagc cgaataaggt tgccctaacc ggagacgcca atctggacat gaactccctg
    64561 ttcggtagcc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    64621 gaaaaaggcg cgatcttcct gaaagagatg gaggtggtcg atgcgacggt acaaccggaa
    64681 aaaatgcaaa cggtgatgca aacgttgctt ccctatctga accaggcatt acgcaattac
    64741 tttaaccagc aacctgctta cgtcctgcgc gaagatggca gccagggcga agcaatggcg
    64801 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc atttactgat
    64861 taatcacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    64921 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    64981 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    65041 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc
    65101 aatctggctc cgcccgtgac caccgttgag gctgccgtgg cgtatcgcca acgtattctt
    65161 cacgccgtac ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg
    65221 ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt
    65281 tacccggcaa acgcaaccac taactccagc cacggcgtga cgtcagttga cgcaatcatg
    65341 ccggtattag agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    65401 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct
    65461 ctgcgccagc gcctgaccgc gctgaaagtc gtttttgagc acatcaccac caaagacgca
    65521 gccgactatg tccgtgacgg aaatgaacgg ctggctgcta ccatcactcc gcaacatctg
    65581 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtctaccc
    65641 atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tagtcgccag cggttttaat
    65701 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagctgc
    65761 ggctgcgcgg gctgcttcaa cgccccaatc gcgctgggaa gttacgctac cgtctttgaa
    65821 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat
    65881 gggttaccgg tcaacgacac attcatcgaa ctggtacgtg aagagcatca ggttgctgaa
    65941 agcatcgcac tgactgatga cacgctggtg ccattcctcg ccggggaaac ggtacgctgg
    66001 tctgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa
    66061 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    66121 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    66181 tgcgtttcct gataatgaag gccacgtatc agtacgttat gccgcagcga ataatttatc
    66241 ggttattggc gcgacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    66301 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg
    66361 gtcgccccgg ctttttta
//
LOCUS       DAZFTN010000012        66184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFTN010000012 DAZFTN010000000
VERSION     DAZFTN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(144..1043)
                     /gene="pgrR"
                     /locus_tag="ACHKV2_000943"
     CDS             complement(144..1043)
                     /gene="pgrR"
                     /locus_tag="ACHKV2_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO6938872.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1181..2101
                     /locus_tag="ACHKV2_000944"
     CDS             1181..2101
                     /locus_tag="ACHKV2_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6938873.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2101..3031
                     /locus_tag="ACHKV2_000945"
                     /pseudo
     CDS             2101..3031
                     /locus_tag="ACHKV2_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            3052..3690
                     /locus_tag="ACHKV2_000946"
     CDS             3052..3690
                     /locus_tag="ACHKV2_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6938874.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3981..4709
                     /gene="mpaA"
                     /locus_tag="ACHKV2_000947"
     CDS             3981..4709
                     /gene="mpaA"
                     /locus_tag="ACHKV2_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO6938875.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4684..5649)
                     /gene="ycjG"
                     /locus_tag="ACHKV2_000948"
     CDS             complement(4684..5649)
                     /gene="ycjG"
                     /locus_tag="ACHKV2_000948"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO6938876.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5768..6274
                     /gene="tpx"
                     /locus_tag="ACHKV2_000949"
     CDS             5768..6274
                     /gene="tpx"
                     /locus_tag="ACHKV2_000949"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO6938877.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6318..7859)
                     /gene="tyrR"
                     /locus_tag="ACHKV2_000950"
     CDS             complement(6318..7859)
                     /gene="tyrR"
                     /locus_tag="ACHKV2_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO6938878.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(8007..9068)
                     /gene="ycjF"
                     /locus_tag="ACHKV2_000951"
     CDS             complement(8007..9068)
                     /gene="ycjF"
                     /locus_tag="ACHKV2_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO6938879.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9065..10462)
                     /gene="ycjX"
                     /locus_tag="ACHKV2_000952"
     CDS             complement(9065..10462)
                     /gene="ycjX"
                     /locus_tag="ACHKV2_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO6938880.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10617..11615
                     /gene="ycjW"
                     /locus_tag="ACHKV2_000953"
     CDS             10617..11615
                     /gene="ycjW"
                     /locus_tag="ACHKV2_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6938881.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(11726..12631)
                     /gene="ompG"
                     /locus_tag="ACHKV2_000954"
     CDS             complement(11726..12631)
                     /gene="ompG"
                     /locus_tag="ACHKV2_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO6938882.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12676..13758)
                     /locus_tag="ACHKV2_000955"
     CDS             complement(12676..13758)
                     /locus_tag="ACHKV2_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6938883.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13772..14431)
                     /gene="pgmB"
                     /locus_tag="ACHKV2_000956"
     CDS             complement(13772..14431)
                     /gene="pgmB"
                     /locus_tag="ACHKV2_000956"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO6938884.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(14428..16695)
                     /gene="ycjT"
                     /locus_tag="ACHKV2_000957"
     CDS             complement(14428..16695)
                     /gene="ycjT"
                     /locus_tag="ACHKV2_000957"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO6938885.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16692..17747)
                     /gene="ycjS"
                     /locus_tag="ACHKV2_000958"
     CDS             complement(16692..17747)
                     /gene="ycjS"
                     /locus_tag="ACHKV2_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6938886.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17757..18545)
                     /gene="ycjR"
                     /locus_tag="ACHKV2_000959"
     CDS             complement(17757..18545)
                     /gene="ycjR"
                     /locus_tag="ACHKV2_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6938887.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(18563..19615)
                     /gene="ycjQ"
                     /locus_tag="ACHKV2_000960"
     CDS             complement(18563..19615)
                     /gene="ycjQ"
                     /locus_tag="ACHKV2_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO6938888.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19646..20488)
                     /gene="ycjP"
                     /locus_tag="ACHKV2_000961"
     CDS             complement(19646..20488)
                     /gene="ycjP"
                     /locus_tag="ACHKV2_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6938889.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20475..21356)
                     /gene="ycjO"
                     /locus_tag="ACHKV2_000962"
     CDS             complement(20475..21356)
                     /gene="ycjO"
                     /locus_tag="ACHKV2_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6938890.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21377..22669)
                     /gene="ycjN"
                     /locus_tag="ACHKV2_000963"
                     /pseudo
     CDS             complement(21377..22669)
                     /gene="ycjN"
                     /locus_tag="ACHKV2_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(22683..24361)
                     /gene="ycjM"
                     /locus_tag="ACHKV2_000964"
                     /pseudo
     CDS             complement(22683..24361)
                     /gene="ycjM"
                     /locus_tag="ACHKV2_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(24571..24885)
                     /gene="pspE"
                     /locus_tag="ACHKV2_000965"
     CDS             complement(24571..24885)
                     /gene="pspE"
                     /locus_tag="ACHKV2_000965"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO6938891.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(24960..25181)
                     /gene="pspD"
                     /locus_tag="ACHKV2_000966"
     CDS             complement(24960..25181)
                     /gene="pspD"
                     /locus_tag="ACHKV2_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO6938892.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25190..25549)
                     /gene="pspC"
                     /locus_tag="ACHKV2_000967"
     CDS             complement(25190..25549)
                     /gene="pspC"
                     /locus_tag="ACHKV2_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO6938893.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25549..25773)
                     /gene="pspB"
                     /locus_tag="ACHKV2_000968"
     CDS             complement(25549..25773)
                     /gene="pspB"
                     /locus_tag="ACHKV2_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO6938894.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25827..26495)
                     /gene="pspA"
                     /locus_tag="ACHKV2_000969"
     CDS             complement(25827..26495)
                     /gene="pspA"
                     /locus_tag="ACHKV2_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO6938895.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26662..27639
                     /gene="pspF"
                     /locus_tag="ACHKV2_000970"
     CDS             26662..27639
                     /gene="pspF"
                     /locus_tag="ACHKV2_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO6938896.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27770..29035)
                     /gene="puuE"
                     /locus_tag="ACHKV2_000971"
     CDS             complement(27770..29035)
                     /gene="puuE"
                     /locus_tag="ACHKV2_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO6938897.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(29073..30353)
                     /gene="puuB"
                     /locus_tag="ACHKV2_000972"
     CDS             complement(29073..30353)
                     /gene="puuB"
                     /locus_tag="ACHKV2_000972"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO6938898.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     GVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30355..31842)
                     /gene="puuC"
                     /locus_tag="ACHKV2_000973"
     CDS             complement(30355..31842)
                     /gene="puuC"
                     /locus_tag="ACHKV2_000973"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO6938899.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(31992..32549)
                     /gene="puuR"
                     /locus_tag="ACHKV2_000974"
     CDS             complement(31992..32549)
                     /gene="puuR"
                     /locus_tag="ACHKV2_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO6938900.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32576..33340)
                     /gene="puuD"
                     /locus_tag="ACHKV2_000975"
     CDS             complement(32576..33340)
                     /gene="puuD"
                     /locus_tag="ACHKV2_000975"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO6938901.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33552..34970
                     /gene="puuA"
                     /locus_tag="ACHKV2_000976"
     CDS             33552..34970
                     /gene="puuA"
                     /locus_tag="ACHKV2_000976"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO6938902.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35119..35283
                     /locus_tag="ACHKV2_000977"
     CDS             35119..35283
                     /locus_tag="ACHKV2_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6938903.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            35273..36658
                     /gene="puuP"
                     /locus_tag="ACHKV2_000978"
     CDS             35273..36658
                     /gene="puuP"
                     /locus_tag="ACHKV2_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO6938904.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            36792..37037
                     /gene="ymjA"
                     /locus_tag="ACHKV2_000979"
     CDS             36792..37037
                     /gene="ymjA"
                     /locus_tag="ACHKV2_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO6938905.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37350..38993
                     /gene="sapA"
                     /locus_tag="ACHKV2_000980"
     CDS             37350..38993
                     /gene="sapA"
                     /locus_tag="ACHKV2_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO6938906.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            38990..39955
                     /gene="sapB"
                     /locus_tag="ACHKV2_000981"
     CDS             38990..39955
                     /gene="sapB"
                     /locus_tag="ACHKV2_000981"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO6938907.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            39942..40832
                     /gene="sapC"
                     /locus_tag="ACHKV2_000982"
     CDS             39942..40832
                     /gene="sapC"
                     /locus_tag="ACHKV2_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO6938908.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40832..41824
                     /gene="sapD"
                     /locus_tag="ACHKV2_000983"
     CDS             40832..41824
                     /gene="sapD"
                     /locus_tag="ACHKV2_000983"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO6938909.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41826..42632
                     /gene="sapF"
                     /locus_tag="ACHKV2_000984"
     CDS             41826..42632
                     /gene="sapF"
                     /locus_tag="ACHKV2_000984"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO6938910.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(42684..43847)
                     /locus_tag="ACHKV2_000985"
     CDS             complement(42684..43847)
                     /locus_tag="ACHKV2_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO6938911.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(43856..45229)
                     /locus_tag="ACHKV2_000986"
     CDS             complement(43856..45229)
                     /locus_tag="ACHKV2_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO6938912.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(45233..48335)
                     /locus_tag="ACHKV2_000987"
                     /pseudo
     CDS             complement(45233..48335)
                     /locus_tag="ACHKV2_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(48335..49456)
                     /locus_tag="ACHKV2_000988"
     CDS             complement(48335..49456)
                     /locus_tag="ACHKV2_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6938913.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(49605..50171)
                     /locus_tag="ACHKV2_000989"
     CDS             complement(49605..50171)
                     /locus_tag="ACHKV2_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6938914.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            50648..51436
                     /gene="fabI"
                     /locus_tag="ACHKV2_000990"
     CDS             50648..51436
                     /gene="fabI"
                     /locus_tag="ACHKV2_000990"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO6938915.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            51580..52707
                     /gene="yciW"
                     /locus_tag="ACHKV2_000991"
     CDS             51580..52707
                     /gene="yciW"
                     /locus_tag="ACHKV2_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO6938916.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            52775..54709
                     /gene="rnb"
                     /locus_tag="ACHKV2_000992"
     CDS             52775..54709
                     /gene="rnb"
                     /locus_tag="ACHKV2_000992"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO6938917.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLYSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(54828..54890)
                     /locus_tag="ACHKV2_000993"
                     /pseudo
     CDS             complement(54828..54890)
                     /locus_tag="ACHKV2_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54944..56929
                     /gene="pdeR"
                     /locus_tag="ACHKV2_000994"
     CDS             54944..56929
                     /gene="pdeR"
                     /locus_tag="ACHKV2_000994"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6938918.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(56911..57039)
                     /gene="ymiD"
                     /locus_tag="ACHKV2_000995"
     CDS             complement(56911..57039)
                     /gene="ymiD"
                     /locus_tag="ACHKV2_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO6938919.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            57077..57250
                     /gene="yciZ"
                     /locus_tag="ACHKV2_000996"
     CDS             57077..57250
                     /gene="yciZ"
                     /locus_tag="ACHKV2_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO6938920.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            57340..58089
                     /gene="yciT"
                     /locus_tag="ACHKV2_000997"
     CDS             57340..58089
                     /gene="yciT"
                     /locus_tag="ACHKV2_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO6938921.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            58358..58576
                     /gene="osmB"
                     /locus_tag="ACHKV2_000998"
     CDS             58358..58576
                     /gene="osmB"
                     /locus_tag="ACHKV2_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO6938922.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(58702..59028)
                     /gene="yciH"
                     /locus_tag="ACHKV2_000999"
     CDS             complement(58702..59028)
                     /gene="yciH"
                     /locus_tag="ACHKV2_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO6938923.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(59028..59765)
                     /gene="pyrF"
                     /locus_tag="ACHKV2_001000"
     CDS             complement(59028..59765)
                     /gene="pyrF"
                     /locus_tag="ACHKV2_001000"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO6938924.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(59958..61127)
                     /gene="lapB"
                     /locus_tag="ACHKV2_001001"
     CDS             complement(59958..61127)
                     /gene="lapB"
                     /locus_tag="ACHKV2_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO6938925.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(61134..61442)
                     /gene="lapA"
                     /locus_tag="ACHKV2_001002"
     CDS             complement(61134..61442)
                     /gene="lapA"
                     /locus_tag="ACHKV2_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO6938926.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(61591..62355)
                     /gene="pgpB"
                     /locus_tag="ACHKV2_001003"
     CDS             complement(61591..62355)
                     /gene="pgpB"
                     /locus_tag="ACHKV2_001003"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO6938927.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            62525..63115
                     /gene="ribA"
                     /locus_tag="ACHKV2_001004"
     CDS             62525..63115
                     /gene="ribA"
                     /locus_tag="ACHKV2_001004"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO6938928.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(63179..65854)
                     /gene="acnA"
                     /locus_tag="ACHKV2_001005"
     CDS             complement(63179..65854)
                     /gene="acnA"
                     /locus_tag="ACHKV2_001005"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO6938929.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(66018..66113)
                     /gene="ymiC"
                     /locus_tag="ACHKV2_001006"
     CDS             complement(66018..66113)
                     /gene="ymiC"
                     /locus_tag="ACHKV2_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO6938930.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
BASE COUNT        16066 a        17562 c        16284 g        16272 t
ORIGIN      
        1 cgggcttcac acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
       61 gaagaataaa aaataaccaa aacgttgaaa ggaactgaga atacgttaag agaagaatta
      121 aattaatgta tcagcaagta gtgctaaaca gcacctttat acttcagcct atctataaat
      181 aatgagaatg ccgagccagc atgtcgacgg tgtggatagt acaggtgata accgggtaaa
      241 tctggtgtga atttatccaa aacacgtatc agtttttttt ctttaatcgc ccgctcaacc
      301 tgatcataag gtagatacgc caatccatgc ccatcaattg cagcatcaat gatcaggtct
      361 atcgtattca gtaaaagctg accttccatg cgaacacgaa cttcacgtcc accgcgtatt
      421 aatctccagc gatttgctgt acccgatgtg ggaagataca aattaattgc ctgatgatct
      481 attaattgtg acactgacgt tggaacactt cggcgagaaa aataatccgg tgagccaaca
      541 atagccattg gtatatctgg cccgattcga atggcgatca tatctttatc catctcccca
      601 cccagacgga cgcctgcatc aaaacgttca gaaacgacat cagtcaaacc ataatcaatg
      661 ataagctgaa tatcaatttc agggtgtgat ttcaggaatg tgcgcatggc tggtaacaat
      721 atcgtttttg ctgcatgttc tacagtagta atacgtattg tcccggatgg gcggttctgc
      781 agatcgctca gggatgccag ggcagaatct atgtcatgca gcatagggcc aagaacagac
      841 aaaagatgct cgcccgcttc agtgggtaca acactgcgcg tggttcgcgt cagaagccgc
      901 aatcccaatc gttcttctat acggcgcact atctggctta aagctgactg cgccatgctc
      961 aggcgggctg ctgcgcgagt gaagctacgc tcctctgcaa cgacgacaaa cgccatcaga
     1021 tcagcgatct cttcacgctt cattcagtat ctccatacaa tctattaatc atttaaatga
     1081 taggtctaat catgatttag ggtctaatca acttaatgat caatatctat agtgatatca
     1141 ccaaccgtta aaacgaacca acaactccat gaggaacgga atgatgaata ataaagtcag
     1201 cttcactaac agcaataatc caaccatctc tttgtctgca gtgatctatt ttcctccaaa
     1261 atttgatgaa acccgtcaat atccggcaat tgtggtatct cacccaggtg ggggcgttaa
     1321 agaacaaacg gccgggacct atgccgaaaa actggcggaa aagggatttg tcactattgc
     1381 ttatgacgca tcttatcagg gtgaaagtgg cggcgagccg cgtcagttag aaaacccata
     1441 cattcgtact gaagacgtca gtgccgtgat tgattacctg acaacgcttt cttatgtcga
     1501 caatacccga attggtgcga tgggaatttg tgccggtgca ggatatacag ccaatgctgc
     1561 aattcaggat cgccgcatca aggccatcgg taccgttagt gcggtcaata ttggttcgat
     1621 gttccgtaat ggttgggaaa acaatgtgaa atctattgat gccctgccat acgttgaagc
     1681 gggttcaaac gccagaacca gcgatattag cagcggagaa tacgccatta tgccactggc
     1741 accaatgaag gagtccgatg cgccgaacga agaactgcgt caggcctggg agtattacca
     1801 cacccctcgc gcgcagtatc caacagcacc gggttacgct actttgcgca gccttaacca
     1861 gattattacc tatgatgctt accatatggc ggaagtgtac ctgactcagc cgacgcagat
     1921 tgtggcgggc agccaggcag ggagcaaatg gatgagtgac gacctgtacg atcgggcatc
     1981 aagccaggat aaacgctatc acattgttga aggcgcaaac catatggatc tttatgacgg
     2041 caaagcctat gtggctgaag caatttcagt attagctccg ttctttgagg aaactctgta
     2101 atgaaacata ttcaaatccg caatagtgat atggcctggc atattgctgc caatattcag
     2161 tttccaccaa attttgatga gtcaaaacag tatccggcga ttatcagcgt ccatcctttt
     2221 ggcagttgta aggagcagac gtctggcaat atctatggca aggcattggc tgaaaaaggc
     2281 tatgtcgttc tcgcttatga cgccagtttc cagggagaat ctggtgggga gcctcgttgg
     2341 atagaagatc cgacacagcg cgtagaggat attagccgtg ttatcgatta tgcagtaact
     2401 ctgccatatg ttgatgctga acgcattggc gtgctgggtg tgtgtggggg ggggtggcta
     2461 tgccattaat gccgcgctga cagaaaaacg aattaaagct gtagtgagta ttacgggtgt
     2521 taatatcggg cgtctttttc gggaaggttt cagcaattac gatccgattg gcgcattgaa
     2581 tgcaatggcg tcgcaacgaa ccaaagaggc tcgtggtgga gaattacaga tcaacgaatt
     2641 acttcccgcc agcctggatg cagcaaaagc ccatggactg accgaacgcg atgtttatga
     2701 agcaactgac tactataaaa caccacgcgg gcaacaaccg ggaggtgcga caaaaatgtt
     2761 gttctcacat gcacagaaaa cgctggcctg ggatgccttc gcatttactg aagtgttact
     2821 gactcagccg gtgatggtag tagttggtga aaaagtcggt gccttcggtg cttatcgtga
     2881 tggactggaa gtctatggcc gggcgatggt ttcgcaggat cgtcagttag tttcattacc
     2941 cgatttctca cattatgaac tgtatgacaa accggaagca gttcaggagg cgctggaaaa
     3001 agttattcct tttttcaaca cccatctgtg ataagtttca tgagaaatat tatgaaaaat
     3061 gtactcattc ttggtgctgg cggtcagata gcccgccatg tgattaatca actggcagat
     3121 aaacaaacca ttaaacaaac tttatttgct cgtcagccag caaaaataca caaaccctat
     3181 ccaactaata gccagattat tatgggggat gtactgaatc atgcagcact gaagcaggcc
     3241 atgcagggac aggatattgt gtatgccaac cttacggggg aagatttaga tattcaggca
     3301 aattcagtga tcgctgcgat gaaagcctgt gatgttaaac gtctgatctt tgttctttcc
     3361 ctcggcattt atgatgaagt tccaggaaaa tttggcgaat ggaataatgc agttattggt
     3421 gagcccctaa aaccattccg cagggctgcg gatgcaattg aggcttctgg actggaatat
     3481 acaattcttc gccctgcctg gctgacggat gaagatatta ttgattatga gcttacctca
     3541 cgcaatgaac cattcaaagg tacgatcgta tcacctaaga gtgtcgcagc attaattaca
     3601 gatatcatcg ataaacctga gaaacacatt ggtgagaata taggcatcaa tcaacccggg
     3661 actgatggcg acaagccatt cttcatgtaa atcaattata taaccaccat cgtactgtac
     3721 tatgtgcagt acgatttatt aaacttatta aggtgaactg atcaatcaga tgcgattaac
     3781 atgtgtagaa ccagtaagaa taaaatacgc catgtatata acattacgac aattctaata
     3841 tctgcacatc aatgaaaata caaacaactt ccggcaaaaa agtgatacaa gatcgatgac
     3901 atagcgttga agaaaaggat ttgataatcc gcaaaatttg cacaacactg cctgtaatac
     3961 ttcctgaatc agagacgatc atgaccgtaa cccgcccacg cgccgaacgc ggcgcatttc
     4021 cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc tggtttccgg
     4081 cccctgcagc cagtcgcgaa agtggtttga ttctggctgg cactcacggt gatgaaaact
     4141 cttcggtcgt cacgctctcc tgtgctctgc ggactttgac accttctttg cgccgccatc
     4201 atgtggtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc aatgctaatg
     4261 gtgtggattt aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa acggtttatc
     4321 gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac aaacctggct
     4381 ccgaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct gcctgggtgg
     4441 tctcattcca cgatccactg gcctgtattg aagatcccag acacagcgaa ttaggcgaat
     4501 ggctggctca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa acgcctggct
     4561 ctttcggcag ttggtgtgca gatctgaact tgcattgcat caccgcagag tttccgccca
     4621 tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc
     4681 atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag
     4741 ccaggtcggt ccgtcaagat cggcgaaact gacctgcggc accagcggta aagcggcgct
     4801 aatggcccga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc
     4861 ttcagtcgcc agcgccagcg cttccgtcag acccccggtt ttatcgagct taatattaac
     4921 catctcatag cgccctttca gcgccttcaa attgctacga gtatgacaac tttcatcagc
     4981 acaaatcggc aacggatgaa taaaattctc cagcgccgca tcgtcctgcg caggaagcgg
     5041 ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgtgccg ccaacccttc
     5101 tgcatgccag gattcatttg catcaacgat cagcgtggta tcgggcacag ctgtgcgaat
     5161 tgccaccatc cgctcactga taagatggtt atccagcttc actttcagta atttcgcgcc
     5221 ggcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga tgactaccgt
     5281 ctgtgcagtg ataactgtct cgggaagcgt tatgccgatc aaatcagcca gcgattgctg
     5341 ctgttttcgc gcggccagat cccacaatgc acaatccagc gcattacgtg ctgcgccggc
     5401 agggagaatt ttttgcaact cctcccgtgt cagccctttc tctaattgcg gcacgacgct
     5461 cataatttgc gccattaccg aggcatcact ttctccataa cgcggatacg gcgtgcattc
     5521 gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg cttcactgcg
     5581 acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcaa ataccttaac
     5641 ggttctcatc tttactcctt ggtgtcgcgg tggtctgcgg attaagcgca aatagcgttt
     5701 gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt aaacaggaaa
     5761 gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg ccaattccat
     5821 cccgcaggcg ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc tgtctgacgt
     5881 caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga gtattgatac
     5941 cggtgtttgc gccgcatcag tacgtaagtt taaccaactg gcaaccgaga tcgacaacac
     6001 cgttgtgctg tgtatctctg ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga
     6061 aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaatttc tgcaagccta
     6121 cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg ttgtggttat
     6181 tgatgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacca ccgagccgga
     6241 ttacgaagca gctctggctg tactgaaagc ataagttaat atgcctgatg gtgttgcacc
     6301 atcaggcata ttcgcgctta ctcttcgttc ttcttctggc ttaagccata ttcccgcaac
     6361 ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg cgtgctggga
     6421 taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat ttcgtccagc
     6481 gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc cggcaacaaa
     6541 atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata gatagcgttc
     6601 tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt caggtcagcg
     6661 gccagtttcg gacgcggcac gccctgctcg tcggcaaagc gggcgacgaa cagctcagtt
     6721 aacggcatga tgtcctgcgg acagtcacgt agcggcggca ggttgagcgt caacacgttc
     6781 agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac cagattcttc
     6841 tgcgtagcgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg
     6901 aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga catctcacct
     6961 atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt cttcccttcc
     7021 ggagcatgac caaacagctc actctcgacc gcatcttccg gtatagacgc acagttcagc
     7081 gccaggtaag gtttgctcgc tcttgggctt gcctgatggc aggcgtaggc aaaaagatct
     7141 ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc cagtttctgc
     7201 gcctgttcga caacatgctt cattttcggg ctgacagcga caatttgact gaaggcgctg
     7261 acgtcctggg cggcgacatt ttgcaactga cggcccatac gaatcgttga tcgcaacatc
     7321 accaccgcac ctgtcaggac gtgttgatca ttttcatcct gaagataaac aggcgtaatc
     7381 tccatcagga agttctgtcc attaataaca acatgctcgt tatgggaatc ttgcggttcg
     7441 ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt atggttgcgc
     7501 agtcgatcca atttttgccc aaaaagctga cagctcgccg ggttcgccat atccactttg
     7561 cttttcatat cgacagagag cacaggttca ggcaacgcct ccagtaacgc gctcaacgcc
     7621 agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac acctgcaata
     7681 cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc aaaattgagg
     7741 tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct tagcacgagt
     7801 agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc cagacgcatg
     7861 ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt tgaatagcag
     7921 gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt cggcgtaaag
     7981 atatccggtc agcacccgat attgcattat ttttcgctgg gcgttttgcc tttttgcagc
     8041 gtttctttca cctgaccgat aagctgacga cggaaatccc cgaggcgagg tttgtcatcg
     8101 tcaatccacg gcagcgggcg gcaaagctcc atagctttaa tccccagtcg tgccgtcaga
     8161 agtcctgcac caatcccctg agctgcgcgg gtagacaaac gagcagcgag atcttgcgac
     8221 atccagtcca ttcccacttc gcgcaccaat tcgctggctc cggcgaaagc gatattcagc
     8281 aataccagct tgaacaggcg caaacggctg taatatccca gttcaatgcc atacagcgtg
     8341 gcgatgcgat taatcaaacg cagattgcgc caggcgataa atgccatatc caccagcgcc
     8401 agcgggctga ccgcaatcat caacgttgat tcagctgctg agcggctgat ttcgcgccgc
     8461 gcctgggcat ctaaaaccgg ctggaccaga tgagcataca ggctgaccac ttcacgatcg
     8521 ttctgcgttt catggattga ggcataccag cgttgcagcg ctggatgaga ctgatcaata
     8581 cccgcctgct gcgccagttt ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctgtgc
     8641 aacaaatcgc gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcgcca taagcgccgc
     8701 cactctgtta ccacagaacc tacgccagcg ccgataatca atgccccagc agcacatcca
     8761 cccagcgcca cccagtcctg agtttgccag gcattcattg tccactgtac accctgcccg
     8821 acaacgcttg cgccaaacag agccagcccg cccatcacca ttttgcgcca caggctgcgt
     8881 ttcggacgta acgctgcgtc cattaccgct tcgacttgcc cctcttcttc ctgcgcttcg
     8941 tcgagcgtgg ccggggcaaa attttgcgcc tgattttcgt caaaggtctg ctgcgccctg
     9001 aattttggat tctgttcgac ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt
     9061 tcggtcatcg caatttatct cctattaaaa attccagcgc ggcatcaaga cgaatatgcg
     9121 gcagcggttt gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct
     9181 tatcccagaa cgcctgacca ggcaaacgtg cgggaacttc gccaggataa acagtgagcg
     9241 gtgcgccatc gctaagtcga ttaccgcgca gcgccgggat tttctcaccg ttaacatcaa
     9301 taatgccact ggtggtcgcc tgaactgacg ccagccccag gcaatccatg ctgatccctt
     9361 cgaacgccgc attttgccag gcatcctgaa ttagttgttg cagcaatgaa accatattgg
     9421 cgtgctgatc gatggtcaca tggtccgctt tagtggcagc aaacaatagc ttatcgataa
     9481 ccggcgaaaa caaacgcctg aacagggtac gctgcccata gtggaaactt tgcatcagct
     9541 gcgtcagcgc cagacgcata tcattaaatg cctgtggccc actgttgaga ggttgcaggc
     9601 aatccaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag aaccccttca
     9661 ccaccttctc gcagtaataa ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat
     9721 cggcctgcgc cagtttggac tcgccccagg catcgacatc cggccacggg aagaattgca
     9781 gcgcgggcgc acctgccata tcccccggca agacaaagcg cccaggctga ataaagtgca
     9841 gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg tctgccagcc
     9901 ggttttcgtc ggcaggcgct agcgggtcca gcccttcgca catcattcgc catttcgccg
     9961 accattcgcc gcgctgacca ttgagtaagc ccgtcatctg gcgcgaccag cttaaatagt
    10021 cctgcgccag catcggcagg tcgagcaacc attcgccagg gtaatccaca atctccagat
    10081 acagcgtgga ggtatcctta aagtggcgca gcagcgaatc gttcgattta tagcgtagtg
    10141 ccaggcgaat ttcgctgacg ccgcgcgttg gcgtcggcca ggcgggagga tcgccataca
    10201 gctgcgccaa cccttcgtcg taggtaaaac gcggaatgcc aaagtcacgc tgaggaatgc
    10261 gttttacgcc cagcagacgc tcttcacgca ccgcacttaa tagcggcaaa cgtgctccgg
    10321 catgaatatt gagcaactga ttgaccatcg cagtgataaa cgccgttttg ccgctgcggc
    10381 taagtccggt tacggcgagg cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa
    10441 gttcattttt aagtcgcttc attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca
    10501 ttcataagga tatgaatgtg ggggccaggc cattgattat caactacccg tgttgtcatc
    10561 gacgggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc tgacctatgt
    10621 cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc gtatcacgcg
    10681 tgctgaataa gcaaaccaat atctccccgg aagcgcgcga aaaagtgtta cgggccattg
    10741 aagaattaca gtatcaacca aacaagctgg cccgcgcgct gacctcttcg ggttttgatg
    10801 ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg tttttctctg
    10861 aagttttgca tgccatcacc gccaaagctg aagaagaagg tttcgacgtg atattgcaga
    10921 cgtcgcacac cccggcagaa gacttacaaa aatgtgaaag caaaattaag cagaaaatga
    10981 ttaaaggcat tattatgcta agttcgccag cggatgagtc attttttgcc caacttgata
    11041 aatacgatat tcctgtggtg gtgattggca aagttgaagg tcaatatgcc catgtttatt
    11101 ctgtcgatac cgataatttt ggtgacagca ttgcgttgac cgatgcgtta attgaaagtg
    11161 gacatcaaaa tattgcctgt ctgcatgcac cgcttgatgt ccatgtttca gtggatcggg
    11221 taaatggtta taagcagagt ctggaagatc ataatattgc agtgcgtgat gaatggattg
    11281 ttgatggcgg ttatacccat gaaacagcac tgcaagcagc acggcaatta ttaagtcagt
    11341 caccgttgcc tgaggcagtg tttgccactg acagcctgaa attaatgagc atttatcgtg
    11401 cggcggcaga gaaaaacatt gctattccgc agcagttagc ggtggtgggt tatagcaatg
    11461 aaacgctgtc atttatttta acgcctgcac cgggcggcat cgatgttccg acgcaggagt
    11521 taggtcgaca aagctgtgag ttattattcc gcttaattgc cggaaaaccg tcaccacaaa
    11581 atattaccgt tgccacgcat atgacgctga aataaatacc agcgaccttg cctgaatgcc
    11641 ggatgcgatg ctggcgcatc ttatccggcc tacatttata gatcggataa agtactcgcg
    11701 ccgcatccgg caatttagcc cattatcaga acgagtaatt tacgccgaca cctgcataat
    11761 ggaatttatc gctgtcgcct tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa
    11821 tggaaagacc attctggaaa tcataaccgt aaaataaacc aacacggtta aaatcatggc
    11881 cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc ccaatgcgcg
    11941 tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg
    12001 tggaaaactc accgttatta cggctgtcgt ccatattgaa gccgcgctcg agataatagt
    12061 tcactcgcaa ggcaaccgtt tcgttgaagg tatattgcag acctgtttcc gtttcgacac
    12121 gggtatcagc gtaaccggtc gtgttcaaat cgttggcaaa tttatacatc gacaaccaac
    12181 cgttgaaacg taaatcgtca gtcagtttca catcccagtc tggcgcgatt ttccagcgct
    12241 gcatattcgc cgtgtcttta cccggttcat caacgtagtg ataaccataa ttacggaaac
    12301 cgccggtcag gccgaaactg aaatcatcgc tttcgaggaa ctgataatgt acctccagct
    12361 ccgggcgatc aaaccacgtt ccacgtttac ccgcgctata atctaccggc ccttcctgat
    12421 aataggccag agaaattctc cacggcccgt tggcggcatt aaaatagaca gaaggctccg
    12481 ccagcccatc catatcttcg ccataaccct cgacgttttc tatttcgtac atcgcgccga
    12541 tattaaagtg ccagtcgttc ttttcctcgg cctgtgcgca ggccattccc gcacacatca
    12601 ccagtgcggt acagggtaat aactttttca ttattgttcc ttgtattatc ctgggggaat
    12661 gccttccccc tgtatttagc gaattgctat ttccgtttct gcatcaaaga aatgacattt
    12721 agtcatatca aaatgaatag tgatattttc tcctgcatga taatcattta acgcaccagc
    12781 acggaccact aactcgtgcc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    12841 ttctaccacg ctaattttgg cggaaatatt attttcctct tgcgcgtccg gatgaatatc
    12901 ttccgggcgc attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    12961 taatttctct tcgggaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    13021 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    13081 gggttggtta taaacggttt tcggcgcacc cacttgctga acaatcccgt ctttcataat
    13141 cacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    13201 ggtgttcagt ttctgatgca gcttgctgat ctctgcgcgc atttgtacgc gcagcttggc
    13261 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    13321 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gcttcaggta
    13381 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    13441 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    13501 cgcgtagttc tggaacacca ttgcgatatt gcgtgctttg gctggaacgt cattcattcg
    13561 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    13621 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    13681 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    13741 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    13801 gcggccaggt cagggagtcc gttgagggca acaataattg cgccccggtt aagcctgcgc
    13861 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    13921 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    13981 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcgc
    14041 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacagaagcc agccctaccg
    14101 ggatctgctg tgcgcggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    14161 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgtcc
    14221 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    14281 catcgcggct gatccctttt agggattcgt taaactgcgc atcaatgctg atgccaattt
    14341 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    14401 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    14461 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    14521 ctgcccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    14581 gagcgtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    14641 ctccggtaac gccggattga gatgtaattc accgtcacgc acgctcaccc cggcaaaacc
    14701 ctgaatcgcc cccagccaga tagcgccagt tgcggcagcg tggataccat catcacaact
    14761 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    14821 cagcaggcca cagcgtgcgg caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    14881 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gataccgctg agaactgctc
    14941 cggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    15001 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttagc
    15061 cagattaatc gccggcttag ccataaacga atcatcctgc ggcaaaacgc catcgggctg
    15121 cgtttctggc atccatagct ctttgaggaa catttcggcg cgatggataa acgcatcgtc
    15181 gctacagccg aactggcggg caatattcag cgcctgttga acgttgtagc gggccatata
    15241 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    15301 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    15361 aagtagcgcc atgccttcat gagcaatgaa actttcatcc cccgtggtct gccagtattg
    15421 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    15481 ttgccgcagc ccggtacgaa tgttaatggc ggcaaattct ggcgtctctt cttcgccgct
    15541 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ctgcgccgtg ctttctcctg
    15601 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    15661 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    15721 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg tcgtgagccg gcgtcatgat
    15781 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    15841 cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg caagaagttg
    15901 gtcgtaactc tgctgcgcgc atatttcaag ctggcgaagc gacgcgctgc cccactcgtc
    15961 taaaacggct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    16021 ctcgcctgca tgaagctgcg cactggtatg ttgcagcaaa cggcgctctt tggcggtata
    16081 gcattgctgc acatcaccgc tcaccttaca gcaacagctg atggccacat cactgcgtcc
    16141 atcctgggtg gtgtagatcc cctgcatcag atgctgacca aacacccgca cctgggtttc
    16201 gtcgagatgt tggcgaccgt ggttggtttg cgtggcgtcg atacctgttg aaatcagcac
    16261 tgacgcgtcg gcgtccagtg gcgtaatagt gatttccagc gcaatgagcg gcagttggtc
    16321 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    16381 aacgttgcgg cgtaattcgc cactggcaaa gtcaagctca cgctgccagg cttcgtggga
    16441 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcaggttcac
    16501 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    16561 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    16621 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    16681 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    16741 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    16801 acgtgattga taaaggcttc catgctgccc aaatggcggt tgtcgtctgc catttcccgt
    16861 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    16921 gcaccggctt tatcaccaca aaagctgacg ttcataatcg actgttcgcg gatgttgagc
    16981 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    17041 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    17101 gtgccgatct tttgaaagct atgcgcattc acgcttttca ccgcaggata gcccagcaca
    17161 tacatcgcag catccagcat atgaatgccg atgtcgatca gcgggccacc accctgcagt
    17221 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    17281 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    17341 agcgcaaaac gatggtgaaa gtcgtaggcc agcaccttgc cctgtttgcg cgcggtatcg
    17401 cacatttccc gcgcctgttc tggcgtcatg gcgggcggtt tttcgcacat cacatggcag
    17461 cccgcttcca gcgccatcag ggtatgttcg taatgaaaac ggttaggtga gcagacgcta
    17521 accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac ggatgcattc
    17581 ccgtattttt ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac agccaccagt
    17641 tccagatcgt tgcgggtgca gtagtacgaa gcatgaacct tatccgccac ctggcctgcg
    17701 cctattatcg cgacccgcag tggagagctt gtcattgcac ttttcacaaa gacctcttag
    17761 caggtacgca accaggtcaa cgaatcacgg tacgcctggg caggatcttc tgcccggata
    17821 cgcccttcat acaccacata accctgatag ttatcggcgc gcagctgttc aaacagcgcg
    17881 tggaaatcga gggtgccgct gcccggctgg taacgatggt tatcggcaat atgcacatga
    17941 ccgagcaagt cgcggttgtc atgcagcgcc tgcgccaggt tatcttcttc gatgttcatg
    18001 tgatagaaat cgccgataat ctgcacatgt ttaagattgt tttcgacgct gtaacgacga
    18061 gcatcggcga gggtgttgat catatgatcc tgatagcggt ttaacggttc gagatacacc
    18121 acggttccgg tacgcgcggc gacctgttcc agtacgcgca gggaatcact caccattttg
    18181 cggtcgccgt ccaggctacg cggcgaggtc atcggcggta agcggaaggt aaacatgccc
    18241 cacgctgccg gaacgacgat acctttaccc cccacttctg ccagcgcttc gagaatgcgt
    18301 tcgatctgct ttaagccatt aagacgacgc tcttcgataa agtcaccaat ccagccgtca
    18361 tagccgccgc aggcggtggt caccggtaaa ccggtttctt tgatcgccga tttgacttct
    18421 tcgatgttgt tgaccagcag tttgccgtca atctcaaaac catcgaagcc catctctttg
    18481 atataacgaa atttctccag aatattttcc gggaagaacg cctgattctg tgtgcctatt
    18541 ttcatttttt cattcctttg aattaaaacg tgacgcccat tttgatgctc tgttccggat
    18601 gctgatcgac atactgcatg tagctttccg ggctgttggc aaaggtcact accgggtcga
    18661 ttaaatcttc gcaattgaga taaccgttca tcagcagttt ccagcaggtt tcttcaatac
    18721 gcttacggct ccagcgcgga taatccgggt tcggttcact gcacgcgcga gagaagacaa
    18781 ttttggcgtt attgaaatgc gcttcgcgtc cgaggttaaa accttcagca aacggtttag
    18841 caaacgcgac ataggagatg gtgccaccgt aagccaggcc gcgcagcgcc gactgcagcg
    18901 cgtcggcgta accgctggtt tcgataatca catcggcacc ctgcttgccg gtcagcgttt
    18961 tgatctcttt gcctacgtca gtgccaatgg gattaaggca gaaatccgca ccgtgacgac
    19021 gagcaatatc acaacgatgg gcaataggat cgacgccaat taccaccgac gcgccagcgc
    19081 gtttagccag ttggatggca atttgaccga tcgcgccaag ccctaccacc actacaaaat
    19141 cccctacgcg tacgttggca tcgcgaacgc cactcatggc aaactgcgcc gggtcgtagc
    19201 agacagcgtt tttccaggag ctgccttccg gcattttgcg cagcttgtag ttattcactg
    19261 cgttaatgat gatcgtctcg gagagcgggc cgtagccgca tacgctgtcg cccaccgcgt
    19321 agtcggtaac gtcgctgccg cactcgataa tgtcgccaac caccatgttg ccaagctgga
    19381 atttgccaaa ctcaatgccg cgcggcgcat ctgccggacg cggggtgaac atctgccatt
    19441 cgccgttaaa gtcttcatcg ataaacgggc tggcggcgcg gaagtcgacc acttccgttc
    19501 cgtgtttcgg tgcaccgaaa cgcacgcgga tcttcacttc attggctaaa atcgcccgat
    19561 cttcatactc aaccagcgca gcaacgcgcg gtgctgtggc tactaacttt ttcatgtaaa
    19621 actccttgta aagaaactgg ccgctttagc ccttcacgcc accggcggtc aaaccactct
    19681 taataaaacg ttccgaaagc gcatacataa tcaccaccgg caatgcggtc accagcgagg
    19741 ccgccatcat ccgtccccag atgtaatctg gcgtactgaa cagcgcgttc aggccgactg
    19801 gtaaggtgaa attgctggcg ctggagagga aaatcgacgc aaacagatag tcgttccatg
    19861 ccaccataaa gcagtagacg aatacggata tcagcccgga catcgccagg ggcacggtga
    19921 tacggaagat aatttgcaga cggttgaggc catccatcat cgccgcttct tcgatctcat
    19981 cagggatggt gtcgaagtaa cttttcagca taaacacggc ggtgggcaga gtttgcgtca
    20041 ccatggtaat gatcagcgcc atctcggtgt cgtaaatgcc aagtgcggtg ataattttga
    20101 acagcggcac caccagcaaa atgccggaga acatatacac cgtgtaaaag ctggcgttga
    20161 tggtcatccg gcctttaaag cgcaggcgag aaagcgcgta agctccgaga atgccgagaa
    20221 ataccgccac cacggatgaa accaccgaca ccaccatgct gttacggaag tagtcgacaa
    20281 acgggaaaat caccgggtta aaaatgtcga cataatgctc cagcgtccac tgttgcggca
    20341 gcagcgtagg atgcagcgag atcgcctctt ttgcgctctt gaacgaggtc atcagcatca
    20401 caaaaaacgg aaacagcgtg atgatgagaa acagcgccag cccgcggtaa aaaccgatac
    20461 gactgagtgt gcgtttattt gttgccattg aggttcaccc gtttcctggt cagcagaatg
    20521 acagcgaaaa taatgatgaa gagcaccacc gagatcgccg ccgctttccc taagtcatta
    20581 aaggcaaagg cggttttgta gagatagaca ccaagaatat cgaccttggt tgtcagcaga
    20641 taaacatccg cgaacatgta gaacatccag atggtgcgaa gggtcaccac cgtcgccagg
    20701 accggcataa tcgcgggcag cgtgacgata cgaaaccgtt gccaggcatt agcgccatcc
    20761 atctctgccg cttcgtacag cgatttgtcg atggtctgca aaatcgccag aaacgagata
    20821 aaggcatacg ggaagtagcg ccagatggcg aagatcacca ccagcacaaa actactgccc
    20881 ggattatcga accacagcgg tgcctgctca taaagatgca gaagatcgac gccgaggtag
    20941 ttcacaatgc cgtagccgtt gttgaacatg tatttccagg cgaacaccag cgaaatagac
    21001 ggcgttacgt aggagaggat caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg
    21061 ttgaaaaaca tcgccactgc cagcccgagc acggtgctgc ccgccaccac caacgcggta
    21121 taccagaccg tcatccacag cgagtgccag aagccgggat cggagagaat acgcacatag
    21181 ttgcttaccc caacaaacgt tgactcgatg ttgggattga gcggcagacg taaaaaacta
    21241 atttcgatat tcgacaccat tggccacgca accagaccgc ccagcagtaa taagctgggt
    21301 gcgagaagca acagcgcaaa gggcatatcg gaacgacctg aaaaaagcct gttcattgcg
    21361 gattcctgtc ctgggtttag tgctgttcga tcagttcatc cagctttttc tggctcgttt
    21421 gcagcgtgcc agggagatcg gctttaccta cggtgacgtt atgcaccatg gagctcacca
    21481 ccccagaccc cgtcacatcg cccatgcggg taaatttttt atcccctact gcgccaaaga
    21541 cctgaatatt cggcagttca ccgattaact gatttggcag ttcacccagc gccttaataa
    21601 cgtcgttgtc tttccaggtg gaagtagtga caaccgcttt gttcaccggc agcgccgcgc
    21661 ctggcgacat catcacccag tcggcaatgt tgtctgcctg ctccataaag gtgacaaatt
    21721 tttcagccgc ttccgtttct tcagtctttt gtccggcggt aatggtcagc gaggtcaaca
    21781 tgccgtagac ggcagagttt ttctcggttg gcaccacgaa accgacgttt ttcggatcgc
    21841 cttctttaat cacagccgga aggatatagg tggagtaaat cgccatcggc gcggtgccgt
    21901 tcataaaggc gtctttcact tccatgatgt cgttagaacc cggcatggtg ttggcagcaa
    21961 ggtcgcggta ataggtcagc gcctgcatca tctctggtga atcaagggtg attttgcctt
    22021 cggcgttaaa gacgttggcc tagttggata acgcaaactg ggagaaggat tgttccgtca
    22081 gcacgctttc tgccgtaggc agcgcaatac cgtatttttt attcgccggg tcattcagtt
    22141 tctgtgcaac gtccagcagc tgttgccagt ttttcggctc ctccagcccc gcttttgcca
    22201 gcacatcttt gcgataccag atgccgccaa tccaggcgct gacaggaaca ccggtccatg
    22261 cgctaccatc ttcggtacgc acaatacgca gtacgccatc gtaaaacgcg ccttcaccaa
    22321 cgttgctgat gaccgtggca accgccttgc gatcgataag ctgctctttg tccataactt
    22381 tggcgtagtc atgactggtt tcaatcactt ccggcagcga gccgctacgt gaaagagtga
    22441 tgactttggt gttataagcg tcctcttcga cgggcacttg tttaacggtg atacctgggt
    22501 tttctttttc aaaacgggca atcaatttgc tgataactgc ctgacgctcc tgctccaccg
    22561 aagaatgcat aaattcgatg gatacatggg gtttattttc ttctttacag cctgaaatca
    22621 gggcgcagga aaccagtgct gataacagca cgatttttga tttaatcatg agatgttcct
    22681 ttttatttaa tccacataac ctgccagggg cgcagcgtta ttttattacc caatatatca
    22741 acttcactaa tcagatcccg accgtgcagg ccagtaatat ttacatgctg aatattttta
    22801 ctgacattaa acaatccggt cagacaatta ccctctgcgt tacttctttg aatacgcatt
    22861 acggatgaat taaccgtatc gatggtaaaa ttattatccg gatgaaactc gttatggctg
    22921 cgacgaagtg taattaaacg cgacaactca tgatataccg catgccttaa tgtagcttca
    22981 tcgttcagtt ctcgggttat ctcttcactg tgatattttt tacggttaat cgcacggtta
    23041 tatccgagtt tttcgacacc tgcgtaatca ttacgcgagc ccagaatact ttgaatatat
    23101 atcgctggca caccggggaa acttaacaaa atcgcatggg caaggataaa cctggcgcaa
    23161 cgttcttcat cgctactctc gcggcggctt aacgcatcca tataggtgac atttatttca
    23221 tagggactgc gcgtaccgtc gggattattt ttccagttta ctaatgcacc ttcctgctgt
    23281 aatgcctcga ccagcgctaa tatttcgctt tctggtaaca agccccgcag tgggttcagc
    23341 ccgatgccat cgtgagaggc gaggaagtta aaccaggtcg ttttaccgga gggcaagctc
    23401 agactttgcg cccactggca cagcgcctcg acgttctgtt tttgcaccgc atgtaacacc
    23461 agcggcggca gcgagaactg gtacaccata tgcgcttcgt catcgccttc gccaaagtaa
    23521 gcaatgttat ctttatgcgg aacattcgtc tcagtaataa tcaccgtacc tggcgcgatg
    23581 tcatcaataa tcgaccgtaa cagtttgata atcagatgtg ttttttccag atggatgcag
    23641 cttgttcccg gctctttcca cataaagcca acggcatcca gacggacata ttctgcgccc
    23701 ttttccaggt aacaaagcaa aacatccacc atcgccagca acacgtcagg gctacggtaa
    23761 ttcaggtcaa tttgatcgtc gctaaaggtg gtccataaat ggcgcgtgga attatcatcc
    23821 atttgaaacg gcgttaatag cggtaatgca cgcggacggg tgacagcgct gagatcggtt
    23881 tgcggatcaa ccgcaataaa aaaatcttca aaacctggct gctgttgtaa atagttttta
    23941 aaccattcac tttttgccga catatggttg cagacaaaat caaacattaa atgactgcat
    24001 tcaccgagct gctgaatatc ctgccactac ccgcttcact ggcgacctga tgataatcaa
    24061 ttaccgaaaa tccatcatca gatgaccatg gataaaaagg taataaatga acatgtgaaa
    24121 aaatgctttg cagccattga tggtaaaact gattaaatgt gggtagtggt tttaaatcat
    24181 tactgtgaaa ttgatcggca taggtaatta acacgacatc actttcatcc cagtgttttt
    24241 tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat
    24301 gagttgcggt aaatgttcca ccgtagattt cgtccaggta atccgtaatt ttctgtttca
    24361 tgttgccttt cctggtagcg gtcccatgaa atgagagact actccgcaga aaataaactg
    24421 tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt
    24481 atttgtgagc tgacgcaaat aatctatagg gatgtaaata aggtgtgaca gaaaaaaacg
    24541 gcgcataagc gccgctcatg gtgaattctt ttaacctttg accttcggca ttgcgatgtc
    24601 tttcaggcca ccggcgttct caacgtgggt atatcccatc tcgctaagga tctcttttgc
    24661 ctgccctgat tggcgtccgg cattgcaata cactttcacg gtgtcgtttt tatccggaac
    24721 gacggtggca atgcgctctt tcacttcctt caggggaata ttgatggccc cctgaacgtg
    24781 ctcttgctga tactgctctg gaacacgaac atcgatccag tgttcagcgg cgaaaacggg
    24841 cagtgaaaac accagtgcca gagcaagtaa gcctttttta aacatgtaag cgtccttcta
    24901 tggatgaata ttgctatcca ttatacgcct gagatttaac aatttttgcg aaactcccct
    24961 caccttttat aacgctgcgc cagtttattg gcagcccgac ttaacagcgg ttccagcgcc
    25021 acagccagca acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca
    25081 cccgctgggc catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca
    25141 ggctttacct tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac
    25201 ggaaacggct acgtaacgtg aatgtatcgg aagtgacata acgttccatc tcgcgtaaac
    25261 gcgtttcact tgccgccagt tcacggtcga cttcatccag caattcactg ctggaaggta
    25321 gctgctcacc aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt
    25381 aagcaaccag ggtaaacagc gccagaccga agaaaatcga caacaccacc aggatacgca
    25441 ccagttttac cggtacatca aaatagttgg caatcccggc gcagacgcca cggaccatgc
    25501 cctgctgtgg aatacgccat aattttttat tgagattaat gcccgccatt agcgatccct
    25561 ccagttcgga tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg
    25621 ttttgcttca tcagccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg
    25681 accagaacga ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat
    25741 ggttaatgga atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg
    25801 cgcgttcgga cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat
    25861 tgtgccagtt gctcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc
    25921 gatttttgtt taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt
    25981 tcgaaagatt cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc
    26041 acatcgcgcg acgagtttgc cgcctgatgg cgtaacatca atgcctgctg gcgagcgcgt
    26101 gtttcgctca atttgttttc cagctcgcca atctctttct tcatgcgtgc cagcgtatcg
    26161 tccaccagcg tcacttcatg ttccagggac ttaatcagat cggtcagttt ctgtttttca
    26221 attaacgccg cacgcgccaa atcctctctc tctttcagca gcgccagttc ggctttttcc
    26281 tgccattcaa cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct
    26341 gccagcgcac gcgccgaagt agaacgtact tcaaccaatg tatcttccat ctcctggatc
    26401 atcagacgca ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca
    26461 ttcacgatgt cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcat
    26521 tcaggattgt cctgctgaac tgttaataca atttgcgtgc caatttttta tctttttgat
    26581 ttataaagat ctgattgaag aatcaacagc aacatgccag gatgaattag ctaattacac
    26641 taacaagtgg cgaatttcat catggcagaa tacaaagata atttactcgg tgaggcgaac
    26701 agctttctcg aagtgctgga acaggtttcg catctcgcac cgctggacaa accggtgctc
    26761 atcatcggcg aacgcggcac cggtaaagag ctgatcgcca gccgcctgca ttatctctcc
    26821 tcccgttggc aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg
    26881 gattccgaac tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcca
    26941 gggagatttg aacgtgccga tggcggtacg ctatttcttg atgaactcgc tacggcaccg
    27001 atgatggtgc aggagaaatt attgcgcgtg attgagtacg gtgaactgga gcgcgttggc
    27061 ggcagccagc cgttgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg
    27121 gcgatggtca atgaaggtac ttttcgcgct gacctgctcg accggctggc tttcgatgta
    27181 gtgcaactgc cgccactacg cgaacgtgaa agcgacatta tgttaatggc ggaacacttt
    27241 gccatccaga tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc
    27301 agagaaacat tgctgaatta tcgttggcca ggaaatattc gtgaattgaa aaacgtggtg
    27361 gaacgttcag tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat
    27421 ccctttaaac ggcgtccgcc tgaagacgct atcgccgttt cagaaaccac ctcgcttcca
    27481 acactgccgc tggatttacg tgagtttcag atgcagcagg aaaaagagtt gctgcaactc
    27541 agtttgcaac aagggaaata taaccagaag cgcgcagctg aattgctggg cttaacctat
    27601 catcagttcc gcgcgttgtt gaaaaagcac cagatttagc gaagttgggc aatacaactt
    27661 gccggatgcg gcgtgaacgc cttatccggc atacataatc aatacgtcgt ttgcaggtct
    27721 gataagcgca gcatatcagg cattattgcg ttgcccctgc ttctgcgtat taatctctca
    27781 gcgcatcctg caaaattttc attgccgcat cgaattgcgc atccgggatg gtcagcggat
    27841 acaggaaacg aatcacgttg ccgtatgcgc cacaggtcag tagaagcagt ccctgcgcca
    27901 gcgcgcgttg ctgaattttt tgcgcaatgg cggctgacgg ctcgcccgtt tgcgggtcgt
    27961 taaattctgc cgcaatcatc gagcccagac cacgtaccgc cgcgatggcc ggaacgtttt
    28021 ctttggcatc aatcagggtt ttcgtcagac gctgccccaa ctgcatggcg cgctcacaga
    28081 gtgactcttt gtcgataatg ttgagcaccg cgtgcgcggc agccaccgcc agcggattcc
    28141 cggcgtaggt gccgccaagc ccgcccggcg cgggcgcgtc cataatattc gcgttaccga
    28201 ccacacccga aagcggcatc ccgcccgcga ggcttttcgc catcgtcatt aagtccggct
    28261 tatcggcgta atgatccatc gcaaacagtt tgccggtacg cgcaaagccg ctttgcactt
    28321 catcggcaat catcacaata ccgtgctcgt cgcacaggcg gcgaatggcg gcaaccagct
    28381 cttttggcgc aacgttgaaa ccgccctcgc cctgcaccgg ttcgaaaata atggccgcga
    28441 cctgcttcgc ttcgatgtct gatttaaaca agcgttcgat ggcgtcgagg gagtcctgtg
    28501 ttgaaatgcc gtgtaaatct gacggataag gtacgtgata caccgaacca gggaacgggc
    28561 cgaagccgat tttgtacggc gcgacctttc cagttaacgc catagtcata tacgtacgac
    28621 cgtggaagcc gccgctaaac gcaatcacgc cagggcgtcc ggtatgggcg cgggcgattt
    28681 tcaccgcgtt ttccaccgct tccgcaccgg tggtgaagaa cgcggttttg gcctgcccgc
    28741 tcaccggggc aagggcgttg attttctccg ccagggtgac gtagctttca tatggcacaa
    28801 tctgatacgc ggtgtgggta aactgttgca gctgctgctc caccgccgcg accagatcag
    28861 ggtggcgatg tccggtattc agcaccgcaa tgcctgcggc gaaatcgatg tactcgttgc
    28921 cctcaacatc cttcagcgtg gcgttttcag ccgactgggc gaagaagtta cacatcacgc
    28981 caaccccgcg cggggtggca gaaagacgac gctgatggaa ttcattgttg ctcatgtttc
    29041 acaccttttg actcttcact gttaatcgga aattaaacgc ccagacgatc gcgcaggctg
    29101 taatacgccg cgcccatcgc ggtaaacggt acgcgcagcg tgcgcccgcc ggggaacggg
    29161 taatgcggca gattggcgaa ggcatcgaaa cgttcggcgt cgccgcgcag cagttcggca
    29221 atcaaacgtc cggcgagatg agtacaggtg acgccgtggc cgctgtaacc ctgcatgtaa
    29281 tagatgttgg tatcgaggcg accgaactgc ggcatacgcg acagggtcag caggaagttg
    29341 cccgtccagc gatagtcgat tttcacgccc ttcaactgcg ggaaggtttt cagcagtttc
    29401 ggcaccacaa gacgctcaac gtcatccggg tcgcgcgcgc cgtacaccac gccgccgccg
    29461 tacagcaaac ggttgtcggc ggtaagacgg tagtaatcaa gcagataatt acagtcttcc
    29521 acgccgtagt ttttcgggat cagcgaacgg gctaaatcat cggaaagccg ctcggtggtg
    29581 atcacctgcg taccgcacgg catgctgcgt ttcgccagtt ccggctccac tttatcgccc
    29641 aggtacgcat tcccggcgac aatcacatac ttcgccgtca cctgacctgt ggctgtacgc
    29701 actacggcgg gcgtggtgtg ctggatctgc gtcactgcgg aaagctcata cacgcgcccg
    29761 ccgttcaggc ggatagcgtc cgcttcgcca atggcgaggt tcagcgggtg aatatgcccg
    29821 ccgctgtgat ccagcagcgc accagtgtag cgatcgctgg cgacctcgcg gcgaatggcg
    29881 ttggcgtcca gcatctccag ctgtttattg ccgtagcgtt cccagttctc tttctgctct
    29941 tcaagtgtgg cgagctgttt atcattcatc gccacaaaca gaccgccggg gcggtagtcg
    30001 cagtcaatct gataacgttt gatacgttcg cggatgatct caccaccttc gaacatcatg
    30061 ctgccgagca tacgggcggt gtccatgccg tagctttttt cgataacgtc gatgtcgcgg
    30121 ctgtaggagt tcacaagctg cccgccgttg cgcccgctgg cgccaaagcc gatgcgtgag
    30181 gcttccagaa ccaccacgtc aaagcccgct tccgccagat gcagtgcgga agacagcccg
    30241 gtatagccgc cgccaaccac gcaaacgtcg caggaaattg actcattcag cgtgtcgaat
    30301 ggcgcgtatt tattcgcact ggcggcgtag taactgctgg tatgttctgt catttcaggc
    30361 ctccaggctt atccagatgg ttttcagttc ggtgaatttt tcaagggcat gcagggattt
    30421 gtcgcgaccg ttgccgctct gcttatagcc gccaaacggc acggtcatat cgccgtcgtt
    30481 gtagttattg acgaagacgg aaccggcttt caggcgtcgg ctcatgcggt gcgcgcggga
    30541 gaggtcgcgc gtccataccg ccgcgccaag gccgtactgg ctgtcgttgg caagctgtag
    30601 cgcctgttct tctgatgtga aacgcgtgac caccagcacc ggaccgaaaa tctcttcgcg
    30661 gcttaaggac gcattcgggt ctacatccac aatgatggtc gggccgatgg cgacagccag
    30721 cccggcgtta cggccatcca acaacagttg ccctttgctt tcgccttcct gaataaagct
    30781 atggaccgag tcggcgtggg cgcagtcgat taaggtgccc atggtggttg cgggatcaag
    30841 tggatggccc ggctgccagt tttgcgcctg ctgttttaac agggctaaga attcatcggc
    30901 tatgctctct tccagcaaca ggcgcgttcc ggcgatgcac acctgcccct ggttgtagaa
    30961 gatgcctgct gcggtggcgc ttgccgcctg ttgcaaatcc gggcagtcag cgaaaacgat
    31021 gttggcgctt ttgccgcccg cttccagcca gacgcgtttc atgttgctgt cgcctgcatc
    31081 tttcagcagc tgtttcccgg tacgggtcga accggtaaag gcaatggcgt cgatatcgtt
    31141 atgacgcgac agcgcctgcc ccgcttcatg accaaaaccc gtcaccacgt tcaacacacc
    31201 atccggcaag cctgcttctt tcgccagccc cgcgagacga atcgcactga gcggtgattt
    31261 ttcagacggt ttcaggacaa cgctgttccc tgctgccagc gccgggccaa gcttccagca
    31321 agtcagcagc agcgggaagt tccacggcac gatggcggca atcacgccga ccggttcacg
    31381 cacgatcatc gccagctcat ggctactggt ggtcgccact tcgccataca ctttgtcgat
    31441 cgcttcggcg taccagcgaa tggcgcgcgc cgcgccgggt atatcatcac gcagactgtg
    31501 acgaatcggt ttgccggtgt cgagagtttc cagcagtgcc agctcttcgg cgttggcttc
    31561 tattaaatcg gcgagtttat tcagcaccgc tttacgtttt gccggagaag agagtgacca
    31621 gtcgccgcgt tcaaatacgc cgcgtgctgc gctcaccgca cggtcgatat cgacgctctt
    31681 gccgcgcgca atattcgcca gcggtgcctg ggtaaccgga tcaacggttt caaaggtttc
    31741 attttccgcc gcagcagtat attcaccgtt aataaataag cggttttcaa tggcgagact
    31801 taacgcttta tcctgccagt aagccagatg atgaaaattc attatgactc ctgtttcacg
    31861 tctatcagat atatgcagtt tgtgaacgtc agggcgcaga gatccccggg ccgtatgccc
    31921 gtttatttct ggcgacattc tttatggatt gccgggcgca ttgcgcttac ccggcttaca
    31981 gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga taattcggca aatacctgcc
    32041 gacgtattac tgaaactgtg cgggatgccg gtattaatgg cataactttg ccccgcgacg
    32101 aggtggtaat cctgaccatt aatcgtcaga ataatttcac cttccagtac agtgcctatt
    32161 tcctcaccct gatgcttaat tctttcccca gtggttgtgc ccggctggta cgtttcaaag
    32221 atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca gcttcattga cacaccctga
    32281 ctgcccatct caattaaatc gtcctgatta atgacgacct gcggctcatc aggtttttcc
    32341 ggctcggaaa agaattccga gagtgacaga ccatacacct tcagcagctt ttgcagcgta
    32401 ctgatggcag ggctgacttt atcttgttct atcgtactga tagcactgtg agtcagcccg
    32461 gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct ggcggatttc cgacaagcgt
    32521 tttcctggcg ccagtccctc atcactcata tttgcatttc cttaaactgt agtggtcaga
    32581 gtcgctgttt ttcagcgata tggtgctgac aagcggtgat aaagccctcg aacaatatac
    32641 gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca ctgtacgccc agcgcaaaag
    32701 gatgattgat gacactaacc gcctcaacta agccatccgg cgagcgagct tcaacacgca
    32761 accgtgggct aacgaccttc gccccttgtc catgcagaga gtttacccaa aagttgctac
    32821 attcaggtaa caatgcagac agcaatcccc cctcttcaac ctgaacttca tgcgacggtg
    32881 catattgctg ttccaccggc agttcgggat cttcccgatg ttccagcaat tcaggctgtt
    32941 cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcctgt aaaccccggc
    33001 agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattaacgcc atgctcagaa
    33061 gatcacgctc gggatcggcg tcaggctcat cgccgttttc accatatagg tgcggctgca
    33121 cattgctggg actaccagga aggtaaatgc catcgagttt cggcaaaagt tgttcaagta
    33181 acgacggttc cgccagcgca tgtggcagcg caattggcaa gccgcctgcg tggatgatgg
    33241 cattcaggta cttttcttgc agagtctggg tcgcatgccc cttaagcctg ttcctgcaca
    33301 ttacgacacc gataaccgga ttgttcatta tattttccat tctcaatctc acaaagtgga
    33361 ctaaattatc gccattactg cggtttaaat gacctttatg ttcaatattt tttcaatcta
    33421 gcagtgggtt ttcatttttg caaactcaat ttaacatttg acaaacattt agtttgcata
    33481 cagattcgaa tggtggtcat tatattttac gctttgataa cgagcggaaa acaaaccaaa
    33541 ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag cagtcagaag
    33601 agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc tacccgaata
    33661 cgcaatatgt tgatgtttta cttaccgatt tgaatggttg cttccgtgga aaacgtattc
    33721 ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt cccggcctcg gtatttgcaa
    33781 tggatattct gggcaacgta gtagaagaag caggtctggg tcaggaaatg ggcgagccgg
    33841 atcgtacctg tgttcctgtt ctcggttcct taaccccttc tgccgccgat ccagagttta
    33901 tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac gttgagccgc
    33961 ggaacgttct taaccgcctg tggcagcagc tgcgccagcg cggattgttc ccggtcgtag
    34021 cggtagagct ggagttctat ttactggatc gccagcgcga cgctgaaggg tatctgcaac
    34081 cgccctgcgc gcccggcact gatgaccgca atacacaaag ccaggtttac tccgttgata
    34141 acctcaacca cttcgccgac gtgctcaatg atattgatga actggcgcag ttacagctga
    34201 ttccggcaga tggcgcggtc gctgaggcct cgccgggtca gtttgaaatc aacctttacc
    34261 atactgataa cgtgctggaa gcctgcgatg atgcgctggc actaaaacgc ctggtgcgtc
    34321 tgatggcaga aaagcataag atgcacgcca cttttatggc gaagccgtat gaagagcacg
    34381 cgggcagcgg aatgcatatc catatcagta tgcaaaataa tcgtggcgag aacgtgcttt
    34441 ctgacacaga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg atgattgacc
    34501 tgatgccgtc atcaatggcg ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc
    34561 cggggatgta tgtaccgacg caggcgtcgt ggggccataa taaccgtacc gtcgccctgc
    34621 gtattccatg cggcgatcgt cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg
    34681 ccaacccata tctggtgatg gcagcgattt ttgccggtat tttgcatggc cttgataacg
    34741 agctgccgtt gcaggaagaa gtcgaaggta acgggctgga acaggaaggc ttacccttcc
    34801 cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt
    34861 taggcgaacg gttttgccat gtgtatcacg cctgtaaaaa tgatgagctg ttgcagtttg
    34921 agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcatag taccccttgc
    34981 ggggttgttt catcggcctg agcggagcca ggcctgacat ttgctggtaa tccagacatt
    35041 tcccggatgt cgcgttatgg agcgcaggca gcaacgggct tccgttaacg attaagactg
    35101 tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgcggg aggctgcgta
    35161 tgcgcccctc ctgcgactga ctggactccg ccccctttgc ttcccttgct aaacaggttt
    35221 gatttcaggt cgacacgacc gcaaacctta ttacgcaggg gaggcagcaa ttatggctat
    35281 taattcacca ctgaatattg ctgcgcaacc cggcaaaacc cgtctgcgaa aatcactgaa
    35341 attgtggcag gttgtgatga tgggtctggc ctatctcacg ccgatgactg tgttcgatac
    35401 ctttggcatt gtatctggca ttagcgacgg tcacgtccct gcgtcctatt tgctggcgct
    35461 ggcgggcgtg ctgtttaccg ctatcagcta cggcaaactg gttcgccagt ttccggaggc
    35521 cggttcggcc tatacctacg cgcaaaagtc gattaacccg cacgtcggat ttatggtcgg
    35581 ctggtcatca ctgctggatt atctcttttt gccgatgatc aacgttttgt tggcgaaaat
    35641 ctatctctcc gccctcttcc cggaagtgcc gccgtgggtg tgggtggtaa ccttcgtcgc
    35701 cattttaacc gccgcgaatc tgaagagcgt caacctggtc gctaacttca ataccctgtt
    35761 tgtactggtg caaatctcca tcatggtggt gtttatcttc ctggtggttc agggactgca
    35821 taaaggagaa ggcgttggca ccgtctggtc acttcagccg tttatcagcg agaacgcgca
    35881 cctgatcccg attattaccg gggcgacgat tgtctgtttc tcgttcctcg gtttcgatgc
    35941 ggtgaccaca ctttcggaag agacaccaga cgccgcacgc gtgatcccga aagccatctt
    36001 cctgacggcg gtctatggtg gcgttatctt tatcgcggcg tcgttcttta tgcagctgtt
    36061 cttccccgat atcagccgct ttaaagaccc ggacgccgca ctgcctgaaa ttgcactcta
    36121 cgtcggcggt aagctgttcc agtcgatttt cctctgcacc acgtttgtga acacgttagc
    36181 gtctggcctg gcctcacatg ccagcgtgtc acgtctgctg tatgtgatgg ggcgcgacaa
    36241 tgtgtttccg gagcgcgtgt ttggctatgt gcacccaaaa tggcggactc cggcactgaa
    36301 cgtcattatg gtcggaattg tcgcgatgtc ggcattgttc tttgatttag tcaccgcgac
    36361 agcattgatt aacttcggtg ctctggtggc gtttaccttc gtgaatctgt cggtgtttaa
    36421 tcatttctgg cggcgtaaag gaatgaataa aagctggaag gatcacttcc actatttgct
    36481 gatgccgctg gttggcgcgc tgacggtggg tgtgctgtgg gttaacctcg agtcaacgtc
    36541 actgacactc ggtctggtat gggcttcgtt aggcggcgca tatttgtggt atttgatccg
    36601 ccgctatcgc aaagtgccgc tgtacgaagg tgacagaacg ccagtgagcg aaacgtaata
    36661 gtcggatgcg cgccgcatta tcctgcctga gggtaatgcg ccgcgccatt aactataaaa
    36721 agatgtatga cccggatcac gagtgctatc atccgccctt atgcttagcg cattaacccc
    36781 aggggcaaat aatgaaccac gatatcccgc taaaatattt tgatattgcc gatgagtacg
    36841 cgaccgaatg cgcagagcct gtcgcagacg ctgaacgcac accgctggcc cactacttcc
    36901 agttactgct cacccgtttg atgaacaatg aagagatcag cgaagaagcc cagcatgaaa
    36961 tggctgctga agcggggatt aatcccgtac gcattgatga gatcgcagag tttttgaatc
    37021 aatggggtaa tgagtaatta tttaattgca gacgatcggt cagtatcgtt gtccggataa
    37081 agcgttcacg ccgcatccgg ctaacaatga cacgcactgc agcgtgcttt aatgggtttc
    37141 tcacgatcgt accgctctgc tcctcgctaa gttgttctgt gagttatagt tatagagaaa
    37201 tcacgggtac gaatgttgaa cagatggcga cgccatctgt tcaacgacca accacacggc
    37261 cctcattacg acccctgaac acaaacggta atggaaaatg tcattaaata ttagtaagat
    37321 agcctcttta cataaaatcc cttaatatta tgcgccaggt attatcgtct cttttggtga
    37381 ttgctggact tgtgagtggt caggcaatcg ccgcgcctga atcccccccg catgctgata
    37441 tccgcgacag cggttttgtc tattgcgtca gcgggcaagt caacaccttt aacccatcca
    37501 aagcgagcag tgggttaatt gtcgataccc ttgccgccca gttttatgat cgactgctgg
    37561 atgtcgatcc ctatacctat cgcctgatgc cagaacttgc cgaaagctgg gaagtactcg
    37621 acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt cccgtttcaa aaaaccgcct
    37681 ggtttactcc cactcgcaaa atgaatgccg acgatgtggt gtttaccttc cagcgaattt
    37741 ttgaccgcaa caacccgtgg cataacgtca acggcagcaa cttcccctac ttcgacagcc
    37801 tgcaatttgc cgataacgtg aaaagcgtcc gtaaactgga taatcatacc gttgagttcc
    37861 gtctggctca gccggatgct tcttttttgt ggcacctcgc aacccattat gcttcggtca
    37921 tgtcggcaga atatgcccgg aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc
    37981 aaccggtcgg cactggaccg tatcagttgt cggaataccg cgccgggcaa tttattcgcc
    38041 tacaacgtca tgatgacttc tggcgcggta aaccgttaat gccgcaggtg gtggtggatt
    38101 taggctccgg cggcaccgga cgtctgtcga aactcctgac cggggaatgc gacgttctgg
    38161 cctggcctgc tgccagccag ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc
    38221 tgcgtcctgg gatgaacgtc gcctatctgg catttaacac cgccaaaccg ccgctaaata
    38281 atcccgctgt ccgccatgcg ctggcactgg cgattaataa ccagcgcctg atgcaatcca
    38341 tctattatgg tacggctgaa acggcggcct ctattttacc gcgcgcctcg tgggcctatg
    38401 acaacgaggc taaaattact gaatacaatc cggcgaaatc gcgcgaacag ttgaagttgt
    38461 tggggctgga aaatttaacg ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc
    38521 ccagtccact gaaaactgcc gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag
    38581 tggtgattgt gccggtagaa ggtcgctttc aggaggcgcg gttgatggat atgagccatg
    38641 atctgacgtt atccggttgg gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt
    38701 tactgagctg cgcggcaatt cattcacaga ccaacctcgc ccactggtgc gatccgaaat
    38761 tcgacagcgt gttgcgtaag acgctctcct cgcagcagct ggcggcgcgt attgaagcct
    38821 atgacgaagc gcagagtatt ctggcgcagg aattgcccat tttgccgctg gcgtcgtcat
    38881 tgcgtttgca ggcctatcgg tacgatatca aaggtctggt acttagcccg tttggtaacg
    38941 cctcctttgc tggggtgtat cgcgagaaac aggatgaggt gaaaaaacca tgattatctt
    39001 caccttacgc cgcattttgt tattgattgt caccttgttc ctgctgacct tcgttggctt
    39061 cagcttgagc tatttcactc cacacgcgcc gttacaaggc gcgtcactgt ggaatgcctg
    39121 ggtgttctgg tttaacggcc tgatccactg ggattttggt gtttccagca ttaacggtca
    39181 accaattgcc gaacagttaa aagaggtctt cccggcgacg atggaactgt gcatcctcgc
    39241 cttcggtttt gcgctgattg ttggcatccc cgtggggatg attgccggga ttacgcgcca
    39301 taagtggcag gataacttga tcaacgccat cgccttactg ggcttttcaa tcccggtgtt
    39361 ctggctggcg cttctgttga cactgttttg ttcactcacg ctaggttggt tgcccgtttc
    39421 agggcgtttc gatctgctct acgaagtgaa accgattacc ggttttgcgt tgattgatgc
    39481 ctggctttcg gactcaccat ggcgggatga aatgatcatg agcgcaatcc gccatatgat
    39541 attgcccgtg atcaccctgt cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat
    39601 cagcactatc gaagtgtatg accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc
    39661 gcgctttact attttgcgtc gccacgttct gcataacgcg ttaccgccgg ttattcctcg
    39721 cctgggctta cagttttcca ccatgctgac gctggcgatg atcaccgaaa tggtttttag
    39781 ctggccgggt ttagggcgct ggttaattaa cgccatccgc cagcaggatt acgcagccat
    39841 ttccgccgga gtgatggtgt gtggctcact ggtcattatt gttaacgtga tttctgatat
    39901 tttgggtgcc atggctaacc ctctgaaaca taaggaatgg tatgccttac gatagcgtat
    39961 acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg
    40021 cctctgcgat ggtcggcctg tacggctgcg cggggctggc tgtactgtgt atgtttggcg
    40081 gctggtttgc gccttacggc atcgaccagc aatttctcgg ttaccaatta ttgccgccat
    40141 catggtcgcg ctatggcgaa gtttctttct tcctggggac tgacgacctc ggacgcgatg
    40201 ttttaagccg tttactgagc ggagctgcgc caaccgtggg tggcgcattt gtggtgacac
    40261 ttgccgcgac gatctgtggc ctggtactgg gcaccttcgc cggggcgacg cacggcctcc
    40321 gctcagcggt gcttaaccat attctggata ccttactggc gatcccttcc ctgctgttgg
    40381 caattatcgt tgtagcgttt gccggaccga gtttgtccca cgctatgttt gctgtctggc
    40441 tggcgctgct gccgcgtatg gtacgttcga tttacagcat ggtgcatgac gaactggaaa
    40501 aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac gctgaatatt ctctggtttg
    40561 ctgtgatgcc taacatcacc gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg
    40621 caattctcga tatcgccgcg ctgggctttc tcgatcttgg cgcacaactc ccctcgcctg
    40681 aatggggagc gatgctcggt gatgcgctgg aactgattta tgtcgccccg tggactgtca
    40741 tgctgccagg tgcggcaatt atgattagcg tgctgttggt taacctcctt ggcgacggtg
    40801 tccgtcgtgc aattattgcg ggggtggaat aatgccatta ctcgatattc gtaacctgac
    40861 tattgaattt aaaaccggtg atgagtgggt taaagccgtt gaccgggtaa gtatgacctt
    40921 aaccgaaggt gaaatccgcg gtcttgttgg tgaatccggt tccggcaaaa gtttgattgc
    40981 gaaagcgatt tgtggggtga ataaagataa ctggcgtgtt actgcagacc gtatgcgttt
    41041 tgatgatatc gatttgctgc gtctctccgc acgcgaacgg cgcaaactgg ttggccacaa
    41101 cgtgtcgatg attttccagg aaccgcagtc gtgtctagat ccttcagaac gtgtgggtcg
    41161 ccagttgatg caaaacatcc cagcctggac ctataaaggc cgttggtggc agcgttttgg
    41221 ctggcgcaaa cgccgtgcca ttgaactgct gcaccgcgtg ggaattaaag atcataaaga
    41281 tgcgatgcgc agttttccct atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc
    41341 cattgcactg gcgaatcaac cgcgtctgct gattgctgac gaaccgacaa actcaatgga
    41401 gccaacaacc caggcgcaaa tctttcgcct gctgacgcgt ctaaaccaaa acagcaatac
    41461 cactattttg cttatcagcc atgacttaca aatgcttagc caatgggcgg ataaaattaa
    41521 cgtgctttac tgcggtcaaa cggtggaaac cgcgccgagt aaggagttag tgacgatgcc
    41581 acatcatcct tatacccagg cgctgatccg cgcgatacca gacttcggaa gcgcgatgcc
    41641 gcataaaagt cgcctcaata cgctacccgg cgctatccca ctgcttgaac agttaccgat
    41701 tggttgtcgt ctggggccac gttgcccgta tgcacaacga gaatgcattg tgacgccacg
    41761 tttgacgggg gcgaaaaatc atctctatgc ctgtcatttc ccgctgaaca tggagaaaga
    41821 gtgagatgat cgaaacgctg cttgaagtgc gtaatttaag taaaacgttc cgctaccgga
    41881 ccggttggtt tcgtcgtcag accgtagaag cggtaaaacc cttgagcttt acgctacgtg
    41941 aaggccagac actggcgatt attggcgaga atggttcggg taaatccacg ctggcaaaaa
    42001 tgctggcggg aatgatagaa cctaccagcg gcgagttatt gattgacgat catccactgc
    42061 attttggcga ttattccttc cgcagtcagc gtattcgcat gatttttcag gatccttcga
    42121 cctcgttgaa tccccgtcaa cgtatctcgc aaattctgga ttttccactg cgcctgaaca
    42181 ccaacctgga accggaacag cgtcgcaaac agattattga aacgatgcgt atggttgggc
    42241 tgctaccgga tcacgtcagt tactatccgc atatgttggc ccccgggcaa aaacagcgtc
    42301 tgggtctggc gcgcgcgctg atattgcgcc caaaagtcat tattgccgat gaagccctcg
    42361 cttcactgga tatgtcgatg cgttcgcagt tgattaatct gatgctggaa ttacaggaaa
    42421 aacagggcat ttcgtatatt tatgttaccc agcatatcgg aatgatgaag cacattagcg
    42481 accaggtgct ggtgatgcat cagggcgagg ttgtcgagcg aggcagcacc gcggatgtgc
    42541 tggcatcgcc gctgcatgaa ctcaccaaac ggctgattgc cgggcacttt ggcgaagcat
    42601 tgacggcgga tgcgtggcgt aaggaccgtt agtgttctgc acctcatcct gacaggggaa
    42661 cccccctcag gatgaggtat tcattacttt tgattgtcga ttctggtgag agcaaccagg
    42721 aatgccagtg tataacacac cgtaattgcc agactcattt ttaacagtgt ttctccgcca
    42781 aatcccgaca atggcgcggc tataccgccg aagacaaaca tgctcatccc catcaacgca
    42841 gacgccgttc cagattcctg cgtccctact gcactcatag cctctgcccc gccaacagtg
    42901 ccgatgccgc tacaaaatgc aatagtgaaa aataatgcca ccagtgccgg aatgggcaat
    42961 tgtgtccatg cgcataagac cgtgagcaat gcacacaaaa ttgccgctat caggccacct
    43021 cgcaacaatg tcatcgcatt aatccgtcgc gccagacgcg aaaagatcca tgaagcaatg
    43081 ataagtccga tgccgttaag gccaaacacc aggctaaatt gcattggact aaagccaaat
    43141 tccttctgca acacgaacga cgaagagccg atatacgcaa acaagcctgc cagcataaaa
    43201 ctttgaatca ggcaaaagcg cataaaacgg cggttctgca ccacgcttcg tccggttaat
    43261 agcaatgatg agcccctttt attttctggc aaggtctcat taataaataa cacgcagccc
    43321 agcaacaata cggtgctaat ttcagccatt acccagaata aagtgcgcca gtcaaaagtg
    43381 ctaacaatgt acccgcccag cactggcgac aacaccggtg ccaggccatt caccgtcatt
    43441 aacagcgcaa aaaactgggt caacgttact ccctgatatt tgtcacgagc aatagaacga
    43501 gagagcaccg aaccacccgc ccccgcgatc ccttgaataa aacgccagac caccagccag
    43561 taaatattgt tcgttgtcgc gcacaaaatg gaagaaacaa taaacaatag caacgacaag
    43621 attaacggac gtttacgccc tattttgtcg ctcagagggc caaataacag ttgtccgacg
    43681 ccaagaccga tcagtgatgc agtcagcgtt aattgcgtta tggtcgtggt tgccgccagt
    43741 tgctcagaca tctccggcaa agccggcaaa taaagatcgg tacacatcgg gccgataccg
    43801 gctaaaagac caagaatcaa tgcccatgaa agagagactc tagccatatt attccttact
    43861 tgagtgatga catgccgccg cccagcgact gccagagcgt gattcggttt gtaaaatcag
    43921 tcagttgcag tgccagcagc tcctgctgtg cgctccacaa gctgcgctgc gccgtcaaca
    43981 cggtcaggta atcaccaaca cccgtctgat agcgacgcaa gccaacatcg accgtttgtt
    44041 gctcagcttt aacatactgg cgctgtgcat ccagttgctc ttcaagcgtg gtccgccgcg
    44101 ccagtgcgtt agcgacatct ttaaatgctc tctgaacggt tttttcatag gtggcaatga
    44161 gtccacgctt ttgtgcttct gcgtaacgaa gctgcgccag attacttccg ccagtgaaca
    44221 gcggtaatgt gaccgatggc gtaaatgacc agatctgcat tccgtggctg aacaacgacg
    44281 acaatgcgtc actgccaaca ccagcgctgg cagtcagcga aatcgtcggg aaaaagttag
    44341 cccgcgccgc gccaatatcg gcattggcgc tcttcaaatt atgttccgct tcctggatat
    44401 ccgggcgacg cagcagaacg tcagatgaca ctcccgcagg aaccagactt atcatctgtt
    44461 ccggcaggct ttccagtgtt cccggtagca aattttcctc aagcgttgta cctgccagta
    44521 agttcagcgc atttttatcc tgcatcactt gtgtctgata gctggcaacg ctggcgcgcg
    44581 cttgctgata aacactcatc gcctcactga catctgtcgc tgccgccgtg ccaacctgtt
    44641 gctggcgctg gataattttc aatgagtttt ctgcactggt catcgtttct tttgccaggg
    44701 ccaggttgct gttatcggcc gccagtgtca gccaggcggt gctgatttca gcaatcagcg
    44761 ttaaacgggt gttctgggct gtaaattcac tggcaagcca ggtttctcgc gccgcccgag
    44821 ataaactctg attacgacca aacagatcta acgtaaaact ggacaccgtg ccgtcagcct
    44881 cggcagtggt ttccgtacca ttcgccagcg aacgactgcg ggttgaactt aaggccgcat
    44941 tcaccgtcgg gaataacgac gcattagttt gcccatagag cgcccgtgct gagtcaatat
    45001 cagcaatcgc tttttgcaca tcgcggttac tgttcagtgc aatagtcacc acctgctgca
    45061 atcggggatc gtggatcacc tgctgccagt catggctgat tgcctttccc tggccctgtg
    45121 ctcccggcaa ggtcgccggg attggggatt caggcgtgct gtagtgggga tcgagcgaga
    45181 cacatcccgc actcaacagc accagaaaaa taagagaacg acgcaacata acttactcct
    45241 gacggcgcga tttaccggca aacaaacgct tcaccagtac aaaaaacaga ggaacaaaga
    45301 aaatagccag caacgtagcg gtcagcgtac cgccaataat gcccgtacca atggcgatgc
    45361 ggctgttcgc ccctgagccg gttgctatcg ccagcggcat taccccccgc aataaacgcc
    45421 agcgaggtca tgatgattgg gcgtaaacga gtctgcgccg cgcgtaatgc cgcacgactc
    45481 aaggaataac cctctgcaac cgccgcttca gcaaattcca caatcagaat ggcgtttttc
    45541 gacgacaagc cgatagtggt taatagcgcc acctggaagt aaacatcgtt gtttaaatca
    45601 cgcatccagg ccgccagcgc cgcgccaaga agccccagag ggatcaccag aatgaccgag
    45661 aacgggactg accagctttc gtacagtgct gcaaggcaga ggaaaaccac cagaatagaa
    45721 actgcataca gactcaatgc ctgaccgctg gccagtttct cctgcaatga caaaccactc
    45781 caggcccagg ttgttcccgc aggaagactg tttgccagtt cctccatttt tgtcattgca
    45841 tcgccggaac taaagccagt cgcattttcc ccctgaattt cataggctgc agaaccgttg
    45901 tagcgcacca gtctttccgg tccatacagc cagcgggtgg tcgcaaaagc agagaatggt
    45961 gtcatggcgt tatcactacc gcgcacaaac catttaccta agtctgacgg cgcggaacga
    46021 aattcactgt cgccctgaat gtagactttt ttcacacgac cacggtcaat aaagtcattc
    46081 acataagtac cgccccacgc actggacagg gtgtcggtga catcattcaa actcaaccct
    46141 aatgacaccg ctttattact atcaatatct acctgcaatt gcggcatttg tggcaaatca
    46201 ttggcacgaa cagaatgtaa ctccggactt tgattcgctt tttctatcaa ctgattacgc
    46261 atttgcagta gtgtttcgcg atcggttcca ccgttagcta acaattcaaa cgtaaaacca
    46321 ttgctttgcc ccagcccatc aacggctggc ggcgtcatcg caaataccgt ggcatcacga
    46381 attgtgccca gctctttggt tgcccgtagg gcgatagcct gggcggtgtt ttctgcccct
    46441 ttacgttgag accagttttt caacgaaaca aacgccatcc cggtgttctg tccgctgccg
    46501 ctaaaactga aaccatcaac ggtaaaaatg acatcggtat ttgctttctc gttaatcagg
    46561 aaccagtcaa caatctggcg attcacttct gctgtacggg cagcggtcgc cccggccggc
    46621 agcgtgtact gcaccatgat ttcgccctgg tcttcagtgg gtaagaaact gcccggcagt
    46681 ttccacatca tcagggccat tccaccaccc agtacgagat aaagccccat cgttcgagca
    46741 gcacggcgca ggacataaat tacgcctcgc tgatatttat cttcagtacg gcggtaaaag
    46801 cggtcaaatg cgccgaaaaa gcctttttta tgtggcggaa catgctggag gacggaaccg
    46861 cacagggcgg gagtcaaggt caatgccacc accacggaaa gcagcattgc ggagataatg
    46921 gttatcgaaa actgacgata aattaccccc gtagatcccc caaagaaggc cataggcagg
    46981 aatacggctg acaacaccag cgcaatggca accagtgcgc cagaaatctc gcccattgat
    47041 ttttccgtgg cttcacgcgc gggcaaccct ttgtcacgca taatgcgttc gacgttctcc
    47101 actaccacaa tggcatcgtc caccagcaag ccgatcgcca gcaccatagc aaacaacgtc
    47161 agggtattaa tcgaatagcc aaacaacgca agaacgccga atgtgcccag caaaacgacg
    47221 gggacagcta acgccggaat cagtgtggca cgcaggtttt gcaggaataa atacatcacg
    47281 caaaccacca atacgatggc ttcaaacagc gtctgaatta cgtcctctac agagatttta
    47341 ataaattcag tgctgtcttt cgggtaagcg atgtcgtatc cctgcggcat gttccgctgg
    47401 aattcggcaa tcttatcctt gaccagcgtc gccgtattca gcgcattcgc accgggcgaa
    47461 agcattacag caaccccggc agacgggtga ccgttaagtt tgccgatagc ggtataatct
    47521 tcactgccca tctcaacgcg agccacatct tttatacgaa caactgcgct gtctgactgg
    47581 cttttcacga taatattttt gaactgatcc accgtctgca agcgagactg cgcacgaacc
    47641 gttgcggtca gttgctgagt attcggtgaa ggcaatgccc ctattttccc ggcagtaacc
    47701 tgcacgtttt gcgcttcaat agcactttgc acgtctgaag gcattagcga gtaagacgcc
    47761 agtttggccg ggtcaagcca gatgcgcata gcgtattccg cgccaaagac ttgcagactc
    47821 cccacacctt caacacgcgc cagcgggtcc tgaacgttac taaccaacca gtcggcgata
    47881 tccgaactgg aggctttgtc agtggtgtca tacacggcag caatcagcaa aaagttgctt
    47941 tgtgatttct ccaccgtaac acccgtttgc tgcacttcgc tgggtaggcg cgactccgcc
    48001 tgctgaattt tattctgcac ctgtgcagta tctggatcgg taccttgttc aaaggtcaca
    48061 ttaatactga ccgaaccatc agagctgctg gttgagctga aatagagtaa attatcaagc
    48121 ccagtgagtt gttgctcgat gacctgagtc acgctgttct ccagcgtttc ggcagaagca
    48181 ccagtataag tggctgaaat tttaatggtc ggtggcgcaa cgtcaggata ttgcgcgacg
    48241 ggcaatgtgc gaatagccag aatccccgcc aacataatca aaatggcgat aacccaggca
    48301 aagaccgggc ggcgaacaaa aaagcgcgaa aacatcaggc gttgcctcca ttagcctgaa
    48361 cttcaacagc cttgaccgtc tgccctgaag tgactttggc agaaccttca acaatcagtc
    48421 ggtcgccgct gtgcaggccg tttagcacca gccatttatc accatatgtt tctcccgttt
    48481 cgagcgttcg ctgctctact ttattgtctt tattcaccac cagcgcagtt gcattgcctt
    48541 tagcatcgcg cgtgacgccc tgttgcggcg cgagaatagc gtcttccatc acgccttcat
    48601 cgacacgagc gcgaacaaac attcccggta ataattgctg ttgtggattg gggaaaatcg
    48661 cccgtaatgt caccgaaccg gtagactcat ctacagcgac ttcggtgagt tccagacgcc
    48721 ctttttcgct gtaggttgtg ccatcttcca gaataagtga gacgctcatg gtgtcactgt
    48781 tggtcgccag tgactgttta cgcagacgta ataaatcgac actggagcga gtgaggtcga
    48841 catacattgt atccagacca cgaatagtcg ttaacgctgt atcttgcgac gcggtcacca
    48901 gtgcgccagg ggttaccgac gaaatgccaa tgcgccctga aatcggtgcg gttaccgtgg
    48961 tccagtcaag attaatgcgc gcagtttcca gtgcggcttt tttcgccgct acactggctt
    49021 tatcttgtgc acaggtagac tgagcatcat cagcatcctg ttgtgaaaca ccgttctctt
    49081 tcaccagtcg ggcataacgc tgcgctttct ggcaatcggc ttttaccagt gcctgtgctt
    49141 gttgtaatgc tgcccgagct tcattccatg cagcctgata actggccgca tcaatctggt
    49201 agagcggctg tccagccttg accagatcac cttccttaaa taagcgtttc tggataattc
    49261 ccccaacctg cggacgtact tcggcactga gcgcagcact ggtgcgtccg gttaattcac
    49321 tgactaccga gaccggttga gatttgactg taacaacgcc gacttctgtt tctgacgggg
    49381 atgaattgtt actttgcgta ttatcacaac cagataagag cagcattgcc actacagatg
    49441 ttgctatata cttcattatt ttctccaggg tgagaaatac cctcctcatt aaaattaatg
    49501 aggatatgtc agggatattc agttataacg aacccgcatc gcgaaatgaa tttcgcaatg
    49561 aaataagggg gattaatatc ccccgctatt ggttaattct gttcttattt atttatcaac
    49621 atactaacaa tatcctgata tatttcctgc agatgttgtg gatcgctttt ttgcggtgtt
    49681 aaacgacgat aaaccgtacc ttccatcatg gtagcaaaca cttcaacgca gcaacgaatg
    49741 tgctcctcgc taagatgggg atgcattctt ttcatatgtg cacaaccgtt agcgaacatt
    49801 ctggcgtcag cttcttcaag cattgccata acccgcgggt tgcgtgtggc ttctgcagcc
    49861 acttccagca tcagcgcgtc gtcgtcctca tttaaggatt tacgcaaggc aaggacttcc
    49921 gggaagtgat cggtacgcgc gtcaatgtcc atttgggcaa tacgaaagtc gatgatacgg
    49981 cgaaccatct cttcaataat ggcgtctttg ttggcgaagt aacgatatat ctgacccacg
    50041 ctaagctgag cttcgctggc aatttgcgac atactggcgg catgaaaccc gcagcgacga
    50101 aaacaccgac gggcggcgtt aatgatttca tcctgacgct gtttgtggcg tatttccagc
    50161 ttgctggtca tttctgtctc ctggcctatc acttggtgag aatgatcgtt ctcattttgc
    50221 tgattatacg tttctccaaa gtaatggaaa gtatttttat cttattcatg gtgcatattc
    50281 tgataatgaa gagattgtgg agattctgtg aagatagttg acaatattaa gttggggtca
    50341 attcatcaga aaggctgcta cttgttgtcg ccagttctgt tttcatcatc aaacttatcg
    50401 gagctaatac gaatagtttc atagtcagat tctataagat atttccccaa cctacccctt
    50461 gcaggcgtca agagtagata acgcaaaatg ctctgtaccg cagcttctct ccggtacaga
    50521 aagcgcaact atagccaccc acagccaggt tgattataat aaccgtttat ctgttcgtac
    50581 tgtttactaa aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat
    50641 taaagctatg ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact
    50701 gtccatcgcc tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac
    50761 ctaccagaac gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga
    50821 catcgttctg caatgcgatg ttgccgaaga tgccagcatc gacaccatgt tcgctgaact
    50881 ggggaaagtt tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga
    50941 tcagctggat ggtgactatg ttaacgccgt tactcgtgaa ggcttcaaaa ttgcccacga
    51001 catcagctcc tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg
    51061 ttctgccctg ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt
    51121 tatgggtctg gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg
    51181 tccggaaggt gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcctc
    51241 cggtatcaaa gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg
    51301 taccgttact attgaagatg tgggtaactc tgcggcattc ctgtgctccg atctctctgc
    51361 cggtatctcc ggtgaagtgg tccacgttga cggcggtttc agcattgctg caatgaacga
    51421 actcgaactg aaataatcgt tctgttggta aagatgggcg gcgttctgcc gcccgttatc
    51481 tctgttatac ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg
    51541 cgtcaggata acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc
    51601 ggcaaaagcc actggtatca tgaaacgcaa tccagtacta cggagtatga cgttctgcct
    51661 ctggtcccgg aagccgcaaa ggtcagcgat ccctttctac tcgacgtgat ccttgaaaaa
    51721 gaaacgctgg cccccttcct ttcatggctg gaccctgcgc gtgttcttgc agtggagttg
    51781 ttccctgacc agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtcg
    51841 acggccctga cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg
    51901 cgacttacgc cgctccccgg gcctgattcc accagggaaa gtaatcatcg gttggcacaa
    51961 atcacgcaat atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc
    52021 cagcatctga atgacgtcgg tcttactgcc tgggactgtg tgatcattaa ccaaatcatt
    52081 ggttttattg gctttcaggc gcggacaatt gcgacatttc aggcttatct cgggcatccg
    52141 gtacgctggt tacccgggct ggagatacaa aactacgccg acgcgtcact gtttgctgat
    52201 gaatcattac gctggcgaag cagctatgag gtggaaaaac tacctgaaga acacacaaaa
    52261 agttcaactg cagaactttg ccaactggcc gaaatactct ctctccaccc tatttcactt
    52321 tcccttctcg aaaggttgtt aaacagcaca cgggttaata cacagccgga taatcagctt
    52381 gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat
    52441 tcatcaaatg aatataaaaa aatcagcccc cttctgcgca agggcgaaaa tgaaattaac
    52501 caatgggctg accgtcattc tgttgagcgc gctaccgttc aggcgataca atggctgacc
    52561 cgagcacccg atcgctttag cgccgcccag ttcagccctt tactcgaaca cgaaaaatca
    52621 tcaacgcaga ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta
    52681 aaaatcgcgt tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg
    52741 tcagccgctc taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc
    52801 gcagcttaaa cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac
    52861 agaaaaaggt tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc
    52921 gcagatgaaa aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga
    52981 acgtgaatcc gcagagccag aagaactggt tgaaccgttt ctgactcgtt tcgtgggtaa
    53041 agttcagggc aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc
    53101 cattccttgc cgcgcagccc gtggtctgaa ccacgagttt aaagaaggcg actgggcagt
    53161 tgccgaaatg cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aactgacaca
    53221 atacatcact tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa
    53281 tctggaaaaa gaagcaccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg
    53341 taaagatctg accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga
    53401 tgacgccctt ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc
    53461 cgatccaacc gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc
    53521 attcaccaac tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga
    53581 tctctgctca ctgcgcgcca atgaagtccg cccggtactg gcatgccgca tgacgctctc
    53641 cgctgatggc accattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc
    53701 gaagctggtg tatgaccagg tttctgactg gctggagaat accggtgact ggcagcctga
    53761 aagtgaagca attgccgaac aagtccgttt gctagcgcaa atttgccaac gccgcggcga
    53821 gtggcgtcat aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg
    53881 tgaaaaaggt gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt
    53941 cgaagaagcg atgattgccg ctaacatttg tgcagctcgc gtactgcgcg ataagctcgg
    54001 ttttggtatc tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc
    54061 gttgctgaaa acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt
    54121 ctgcaaactg cgtcgtgaac tggatgcgca accaactggt ttcctctaca gccgcattcg
    54181 tcgcttccag tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct
    54241 ggaagcatac gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg
    54301 tctgctgaaa gcggttatca aaggcgaaac tgcgacgcgt ccacaggatg aaatcactgt
    54361 ccaaatggcc gagcgtcgcc gtctcaatcg gatggcagaa cgtgatgttg gtgactggtt
    54421 atacgcacgc ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt
    54481 cgatatcagc cgtggcggca tgcgtgttcg tttggttgat aacggcgcta tcgcctttat
    54541 tcctgcacct ttcttacacg ctgtgcgcga tgaactggtt tgcagccagg aaaacggcac
    54601 cgtacaaatt aaaggtgaaa cggtttataa agtaactgac gttattgacg tcaccattgc
    54661 cgaagtccgc atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac
    54721 ggcccattcc ttatgaatgg gccgtttatt tccccgctct accttcatca tatcccggca
    54781 gttttttaac tgactttcgt ttgaaaactg gcgtttttcc aaaactagat tacaaataaa
    54841 tatataaaaa aaacaatgtg tttagccgcc gttaatttca tgtaagtcat gttgttcctt
    54901 ttatttaacc gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga
    54961 gtccacaacg ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag
    55021 cagcgatgtt ttgcgctttt ctaccaccga aaccacagaa cctgaacgta cgttgcagtt
    55081 atctgccgaa caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat
    55141 gagtctgacc gtcgatgaga gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa
    55201 acgggaatgg gctggcaacg cgtctgcatg gcatgacaca ccagcagttg ctcgtgattt
    55261 atcacacgga ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat
    55321 tctcgacagc cgggggaata tccaacgctt caatcggtta tgtgaagatt acacagggtt
    55381 gaacgaacac gacgtcattg gccaaagcgt gtttaaactg tttatgagcc gtcgtgaagc
    55441 tgcggcatcc aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga
    55501 actgtggata ccaacacgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca
    55561 cagcggcagt ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga
    55621 gcgccgcgct caggaacgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc
    55681 gaatcgtaac gcaatgcagg agttaatcga tcacgctatt aatcaggcag ataacaataa
    55741 agttggggtc gtgtatcttg atttggataa ttttaaaaag gtcaacgacg cctatgggca
    55801 tttatttggt gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca
    55861 tgaccaggtg ttggcgcgtc caggtggcga tgagtttctg gtactggcat ccaacacctc
    55921 acaaagcgcg ctggaagcaa tggcatcacg aattttgacc cgcttacggc tcccctttcg
    55981 cattggttta attgaagttt ataccagctg ttcagtaggt attgcactct ctcccgaaca
    56041 tggttcggac agcgcggcta ttattcgtca cgccgacaca gcaatgtaca cagcaaagga
    56101 aggcggacga ggacaatttt gcgtttttac cccagaaatg aatcaacggg tatttgaata
    56161 tctctggctg gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta
    56221 tcaacccaaa atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    56281 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgcag aagagtcagg
    56341 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcgaagtg
    56401 gcgggataaa ggcattaacc tgcgagtggc ggtaaacatt tctgcacgtc agctcgccga
    56461 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    56521 tattgatgtt gaattgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
    56581 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccgccta
    56641 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    56701 tgttcgagat attcacaaac aacctgtctc gcagtcactg gttcgggcaa tcgtcgctgt
    56761 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    56821 ttttttaacc aagaatggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    56881 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
    56941 tatatactta gcgctatatt ccgtcattat atatcgtatt cccggatgat aattagccgt
    57001 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc
    57061 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    57121 tgtgttagat atccttgtgg cagacgatta ccactctgcg atccataatc tcgagatact
    57181 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg
    57241 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    57301 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    57361 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    57421 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    57481 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    57541 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    57601 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    57661 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    57721 gcgccttgtg aagttatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    57781 ggcccgttga cacgccagtg catccaacag gtacatttca gcaaagcatt tattggtatt
    57841 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtg
    57901 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    57961 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    58021 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    58081 gacatttaac catttccctt ctctacggat gatttgcagt ttgacaaatc atccgctcta
    58141 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa
    58201 aattattatc agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc
    58261 gagcggattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    58321 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    58381 gaccgcggct gttctggcaa ttaccctggc aatgtctctg agtgcctgtt ctaactggtc
    58441 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    58501 gaccgatggc agtacgttgg gtacattagg tggcgcagct gtcggtggtg ttattggtca
    58561 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgtaatta taatacgctt
    58621 gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg
    58681 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    58741 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    58801 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    58861 atcgagatcg ataccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    58921 ctgaatacgc accacaccat cgcctttaga gcgtactggg gccgctttgg gctcatcaat
    58981 acgcccggtt tctgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    59041 tgtaaagagg cgttgatcgc tttcagcgtc tgcgctggat ctaccgattg cgttaccggg
    59101 cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg
    59161 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc
    59221 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    59281 ccacattttt gcgtcagtgc tgccagacgt tctgcataat ctgcaggtga cagtgtcacg
    59341 ccaagatcgg ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    59401 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    59461 ccagaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    59521 gctgcagtgt tggggatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    59581 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttgcc gaccttcaga
    59641 cgacaatcgc gtgggtcgat cttgtcgaca aaggccagcg cggcatcacg attatgataa
    59701 tcaagggcaa caaccacagg agaattcgta acagcgcggg aagaagatga agcagttaac
    59761 gtcatgacca gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg
    59821 agcattctac ccgtggaaat aaaaaattaa cagttgcgat ttctcccctg gcaggctttt
    59881 tctgcgccgc cgttgccgga gcacaatggg acataaataa ctagtatgtt gtaactaaag
    59941 tcggtttttt taaaaaatta caggccatca agaccgcgaa tcggtttaat agttgaccag
    60001 gcacgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag
    60061 cgataacgag gcttactacg taccttctcg ccaaccatgt cacgcagcac catcaggctc
    60121 tctttggcac gcccttcttc cgcttcattt aagtggtaat ccattaactt atggaacaca
    60181 cgcatggtcg gatgacgctg aagctggcgc gtaatataga cctgtgcggc ctcactaccg
    60241 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    60301 accgcgcgct gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    60361 tgcagcattt ccagcgtttc gctgaccagt tctctgtcct gggatatcac gcgttgcaaa
    60421 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    60481 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    60541 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    60601 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    60661 tcactggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    60721 tcatcggtca gctgattgaa catgtcttcc gcgcggtcat ataacccggc ggccatgtaa
    60781 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    60841 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    60901 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    60961 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    61021 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    61081 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    61141 ttcgccgctg acgagtgctg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    61201 agtcgcttta ttttacgttc agcgcgcacc agggaaacac gaactcgcag ccagaacagg
    61261 ccacaaatca accaaccgat agcaaaaccc gcagcaaaca ataccgccag caatgtggaa
    61321 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    61381 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    61441 acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc
    61501 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    61561 taagggctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gcgctatttc
    61621 gcgattttct tccgcaggtg gtgttaatgg cccgcaaatt cgttgcgcaa gccacgttgc
    61681 caccgccacc agtgcccacg aaatcaacgt agctaccacc agatcgcgtg gccaatgcat
    61741 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accagcaaga tagcaatggt
    61801 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    61861 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    61921 ttgtggaata tttttctctt cagccaactg ttctttcact agatttccgc gttctgctcg
    61981 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccagcc agataacaaa
    62041 aggtcgtggt tcctggactt tgtctttgat ccaggattta acgccttgtc ccacaaggat
    62101 tgcggccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    62161 gagaaaccag ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    62221 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    62281 agaaatccat acggctactg gcataacaag caatagtgca gctcccactg cggtacgtct
    62341 tgcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaatgcc
    62401 agttccagga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    62461 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    62521 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    62581 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    62641 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    62701 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    62761 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    62821 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gatactgtag aggctaacca
    62881 ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    62941 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    63001 cgaagcgggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    63061 cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc
    63121 ttgcattgtg taattaattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt
    63181 acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag taagtcaact
    63241 ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca tccgcgcgcg
    63301 taagcgtcac cggaaccgtc gcgccgggtt gtagattttg cagatcgcca atatcaatct
    63361 tctcttcccc ggttagccct aacgttttac gcgttacgcc ttgcggaaat tccagcggca
    63421 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    63481 cacgaatacc aagcagacgc ggaccttttg ccgcccagtc acggctggag cctgatccat
    63541 actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc atcgcagcat
    63601 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    63661 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    63721 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtaggt
    63781 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    63841 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccaca ggtgctggcg
    63901 ttgcctgcat ttcatcaaag aaaggcgata aacgaatata ggttgaatcc tcctgccatc
    63961 cgtaggtatc ggatcgtgcg acgttaattt ctttccactc tgcagtgcct tcaaagactt
    64021 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    64081 gtgccgatgg ccagatatct ttcagatata ttggatcgcc tttacgatca tgaccaatag
    64141 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggcg
    64201 gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    64261 cggacagcac tgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    64321 gcagcggccc agagttacca atacaggtgg tacaaccgta tcccacaagg ttaaacccca
    64381 gttcgtcgag atacggtgtc agtttcgctt ttgccagata gtcagaaacg actttcgaac
    64441 ccggtgccag cgacgctttg acccacggtt gccgcttgag gccaagagtt acggcttttt
    64501 tcgccagcaa gcctgcggcc atcagcacac ttgggttaga agtgttggtg cacgaggtta
    64561 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    64621 cgaccggctg gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg
    64681 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg tttaggccct gccaggctcg
    64741 cttcaacgtc attcatatcc agatctaacg tactggtaaa aatcggttca tcgcccgggt
    64801 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga tcttcgctgc
    64861 gcccgcttaa acgcatgtaa tcgagggtta cagcatcgat tgggaagaag ccacaggtgg
    64921 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccaacggt agtgaatcca
    64981 gaccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg
    65041 taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg gtaagtttaa
    65101 agcccactac gtccgggata agcatggaaa ccggctggcc taacattgcg gcttctgctt
    65161 cgatgccacc cacgccccac cccagcacgc caaggccgtt gatcatagtg gtgtgcgagt
    65221 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcgctccaca
    65281 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    65341 cgctaaaccg actgaacgct tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    65401 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    65461 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    65521 caccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    65581 taaagtcctg catcagcacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    65641 ttttcagcca tcccgccagc gcgtggatat cctcttcggt aaccgagtta ccatcctgcc
    65701 agcgcagcag attttcgagc aaaactttga gtgacttggg tagacgggtg atatcgccca
    65761 gtgatttggc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    65821 cgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    65881 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    65941 ataacaaccc aaattgataa aagaggagaa accttgtggc aaagcaaatg acaaccccgc
    66001 cgcagcgggg tcaaggatca gtgagtaatg atccagagga gttcggccca gaagagcatc
    66061 gacagagaaa atgcacccat ccaggaccaa tatttcatat ttgtgttgat cataggctac
    66121 acccttaacg gttattactt tgtgttgaga taactcaacc tgttattgct gcggtgtatt
    66181 aaaa
//
LOCUS       DAZFTN010000013        65325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFTN010000013 DAZFTN010000000
VERSION     DAZFTN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..504)
                     /gene="folK"
                     /locus_tag="ACHKV2_001007"
     CDS             complement(22..504)
                     /gene="folK"
                     /locus_tag="ACHKV2_001007"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO6938931.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            complement(501..1898)
                     /gene="pcnB"
                     /locus_tag="ACHKV2_001008"
     CDS             complement(501..1898)
                     /gene="pcnB"
                     /locus_tag="ACHKV2_001008"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO6938932.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1958..2854)
                     /gene="gluQRS"
                     /locus_tag="ACHKV2_001009"
     CDS             complement(1958..2854)
                     /gene="gluQRS"
                     /locus_tag="ACHKV2_001009"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO6938933.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2921..3376)
                     /gene="dksA"
                     /locus_tag="ACHKV2_001010"
     CDS             complement(2921..3376)
                     /gene="dksA"
                     /locus_tag="ACHKV2_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO6938934.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3554..4258)
                     /gene="sfsA"
                     /locus_tag="ACHKV2_001011"
     CDS             complement(3554..4258)
                     /gene="sfsA"
                     /locus_tag="ACHKV2_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO6938935.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4273..4803)
                     /gene="thpR"
                     /locus_tag="ACHKV2_001012"
     CDS             complement(4273..4803)
                     /gene="thpR"
                     /locus_tag="ACHKV2_001012"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO6938936.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            4877..7306
                     /gene="hrpB"
                     /locus_tag="ACHKV2_001013"
     CDS             4877..7306
                     /gene="hrpB"
                     /locus_tag="ACHKV2_001013"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO6938937.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7502..10036
                     /gene="mrcB"
                     /locus_tag="ACHKV2_001014"
     CDS             7502..10036
                     /gene="mrcB"
                     /locus_tag="ACHKV2_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO6938938.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10256..12499
                     /gene="fhuA"
                     /locus_tag="ACHKV2_001015"
     CDS             10256..12499
                     /gene="fhuA"
                     /locus_tag="ACHKV2_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO6938939.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12550..13347
                     /gene="fhuC"
                     /locus_tag="ACHKV2_001016"
     CDS             12550..13347
                     /gene="fhuC"
                     /locus_tag="ACHKV2_001016"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO6938940.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13347..14237
                     /gene="fhuD"
                     /locus_tag="ACHKV2_001017"
     CDS             13347..14237
                     /gene="fhuD"
                     /locus_tag="ACHKV2_001017"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO6938941.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14234..16216
                     /gene="fhuB"
                     /locus_tag="ACHKV2_001018"
     CDS             14234..16216
                     /gene="fhuB"
                     /locus_tag="ACHKV2_001018"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO6938942.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16251..17531)
                     /gene="hemL"
                     /locus_tag="ACHKV2_001019"
     CDS             complement(16251..17531)
                     /gene="hemL"
                     /locus_tag="ACHKV2_001019"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO6938943.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17697..17744
                     /gene="yadX"
                     /locus_tag="ACHKV2_001020"
     CDS             17697..17744
                     /gene="yadX"
                     /locus_tag="ACHKV2_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO6938944.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17756..19177
                     /gene="clcA"
                     /locus_tag="ACHKV2_001021"
     CDS             17756..19177
                     /gene="clcA"
                     /locus_tag="ACHKV2_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO6938945.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19201..19266
                     /gene="yadW"
                     /locus_tag="ACHKV2_001022"
     CDS             19201..19266
                     /gene="yadW"
                     /locus_tag="ACHKV2_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO6938946.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19259..19603
                     /gene="erpA"
                     /locus_tag="ACHKV2_001023"
     CDS             19259..19603
                     /gene="erpA"
                     /locus_tag="ACHKV2_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO6938947.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19650..20273)
                     /gene="yadS"
                     /locus_tag="ACHKV2_001024"
     CDS             complement(19650..20273)
                     /gene="yadS"
                     /locus_tag="ACHKV2_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO6938948.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20311..21111)
                     /gene="btuF"
                     /locus_tag="ACHKV2_001025"
     CDS             complement(20311..21111)
                     /gene="btuF"
                     /locus_tag="ACHKV2_001025"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO6938949.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21104..21802)
                     /gene="mtnN"
                     /locus_tag="ACHKV2_001026"
     CDS             complement(21104..21802)
                     /gene="mtnN"
                     /locus_tag="ACHKV2_001026"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO6938950.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21886..23403
                     /gene="dgt"
                     /locus_tag="ACHKV2_001027"
     CDS             21886..23403
                     /gene="dgt"
                     /locus_tag="ACHKV2_001027"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO6938951.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23533..24957
                     /gene="degP"
                     /locus_tag="ACHKV2_001028"
     CDS             23533..24957
                     /gene="degP"
                     /locus_tag="ACHKV2_001028"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO6938952.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25112..26269
                     /gene="cdaR"
                     /locus_tag="ACHKV2_001029"
     CDS             25112..26269
                     /gene="cdaR"
                     /locus_tag="ACHKV2_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO6938953.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26358..26744)
                     /gene="yaeH"
                     /locus_tag="ACHKV2_001030"
     CDS             complement(26358..26744)
                     /gene="yaeH"
                     /locus_tag="ACHKV2_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO6938954.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27059..27883)
                     /gene="dapD"
                     /locus_tag="ACHKV2_001031"
     CDS             complement(27059..27883)
                     /gene="dapD"
                     /locus_tag="ACHKV2_001031"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO6938955.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27914..30586)
                     /gene="glnD"
                     /locus_tag="ACHKV2_001032"
     CDS             complement(27914..30586)
                     /gene="glnD"
                     /locus_tag="ACHKV2_001032"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO6938956.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30648..31442)
                     /gene="map"
                     /locus_tag="ACHKV2_001033"
     CDS             complement(30648..31442)
                     /gene="map"
                     /locus_tag="ACHKV2_001033"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO6938957.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31810..32535
                     /gene="rpsB"
                     /locus_tag="ACHKV2_001034"
     CDS             31810..32535
                     /gene="rpsB"
                     /locus_tag="ACHKV2_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO6938958.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32793..33644
                     /gene="tsf"
                     /locus_tag="ACHKV2_001035"
     CDS             32793..33644
                     /gene="tsf"
                     /locus_tag="ACHKV2_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO6938959.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33791..34516
                     /gene="pyrH"
                     /locus_tag="ACHKV2_001036"
     CDS             33791..34516
                     /gene="pyrH"
                     /locus_tag="ACHKV2_001036"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO6938960.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34666..35223
                     /gene="frr"
                     /locus_tag="ACHKV2_001037"
     CDS             34666..35223
                     /gene="frr"
                     /locus_tag="ACHKV2_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO6938961.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35315..36511
                     /gene="ispC"
                     /locus_tag="ACHKV2_001038"
     CDS             35315..36511
                     /gene="ispC"
                     /locus_tag="ACHKV2_001038"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO6938962.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36700..37458
                     /gene="ispU"
                     /locus_tag="ACHKV2_001039"
                     /gene_synonym="uppS"
     CDS             36700..37458
                     /gene="ispU"
                     /locus_tag="ACHKV2_001039"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO6938963.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37471..38328
                     /gene="cdsA"
                     /locus_tag="ACHKV2_001040"
     CDS             37471..38328
                     /gene="cdsA"
                     /locus_tag="ACHKV2_001040"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6938964.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38340..39692
                     /gene="rseP"
                     /locus_tag="ACHKV2_001041"
     CDS             38340..39692
                     /gene="rseP"
                     /locus_tag="ACHKV2_001041"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO6938965.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39722..42154
                     /gene="bamA"
                     /locus_tag="ACHKV2_001042"
     CDS             39722..42154
                     /gene="bamA"
                     /locus_tag="ACHKV2_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO6938966.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42275..42760
                     /gene="skp"
                     /locus_tag="ACHKV2_001043"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42275..42760
                     /gene="skp"
                     /locus_tag="ACHKV2_001043"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO6938967.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42764..43789
                     /gene="lpxD"
                     /locus_tag="ACHKV2_001044"
     CDS             42764..43789
                     /gene="lpxD"
                     /locus_tag="ACHKV2_001044"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6938968.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43894..44349
                     /gene="fabZ"
                     /locus_tag="ACHKV2_001045"
     CDS             43894..44349
                     /gene="fabZ"
                     /locus_tag="ACHKV2_001045"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO6938969.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44353..45141
                     /gene="lpxA"
                     /locus_tag="ACHKV2_001046"
     CDS             44353..45141
                     /gene="lpxA"
                     /locus_tag="ACHKV2_001046"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO6938970.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45141..46289
                     /gene="lpxB"
                     /locus_tag="ACHKV2_001047"
     CDS             45141..46289
                     /gene="lpxB"
                     /locus_tag="ACHKV2_001047"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO6938971.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46286..46882
                     /gene="rnhB"
                     /locus_tag="ACHKV2_001048"
     CDS             46286..46882
                     /gene="rnhB"
                     /locus_tag="ACHKV2_001048"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO6938972.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46919..50401
                     /gene="dnaE"
                     /locus_tag="ACHKV2_001049"
     CDS             46919..50401
                     /gene="dnaE"
                     /locus_tag="ACHKV2_001049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO6938973.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50414..51373
                     /gene="accA"
                     /locus_tag="ACHKV2_001050"
     CDS             50414..51373
                     /gene="accA"
                     /locus_tag="ACHKV2_001050"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO6938974.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51472..53613
                     /gene="ldcC"
                     /locus_tag="ACHKV2_001051"
     CDS             51472..53613
                     /gene="ldcC"
                     /locus_tag="ACHKV2_001051"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO6938975.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53670..54059
                     /gene="yaeR"
                     /locus_tag="ACHKV2_001052"
     CDS             53670..54059
                     /gene="yaeR"
                     /locus_tag="ACHKV2_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6938976.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54124..55419
                     /gene="tilS"
                     /locus_tag="ACHKV2_001053"
     CDS             54124..55419
                     /gene="tilS"
                     /locus_tag="ACHKV2_001053"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO6938977.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55472..55732)
                     /gene="rof"
                     /locus_tag="ACHKV2_001054"
     CDS             complement(55472..55732)
                     /gene="rof"
                     /locus_tag="ACHKV2_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO6938978.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55719..55919)
                     /gene="yaeP"
                     /locus_tag="ACHKV2_001055"
     CDS             complement(55719..55919)
                     /gene="yaeP"
                     /locus_tag="ACHKV2_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO6938979.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56085..56630
                     /gene="yaeQ"
                     /locus_tag="ACHKV2_001056"
     CDS             56085..56630
                     /gene="yaeQ"
                     /locus_tag="ACHKV2_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO6938980.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56627..57037
                     /gene="arfB"
                     /locus_tag="ACHKV2_001057"
     CDS             56627..57037
                     /gene="arfB"
                     /locus_tag="ACHKV2_001057"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO6938981.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57051..57761
                     /gene="nlpE"
                     /locus_tag="ACHKV2_001058"
                     /gene_synonym="cutF"
     CDS             57051..57761
                     /gene="nlpE"
                     /locus_tag="ACHKV2_001058"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO6938982.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57961..58785)
                     /gene="yaeF"
                     /locus_tag="ACHKV2_001059"
     CDS             complement(57961..58785)
                     /gene="yaeF"
                     /locus_tag="ACHKV2_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO6938983.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58838..60556)
                     /gene="proS"
                     /locus_tag="ACHKV2_001060"
     CDS             complement(58838..60556)
                     /gene="proS"
                     /locus_tag="ACHKV2_001060"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO6938984.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60667..61374)
                     /gene="tsaA"
                     /locus_tag="ACHKV2_001061"
     CDS             complement(60667..61374)
                     /gene="tsaA"
                     /locus_tag="ACHKV2_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO6938985.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61371..61775)
                     /gene="rcsF"
                     /locus_tag="ACHKV2_001062"
     CDS             complement(61371..61775)
                     /gene="rcsF"
                     /locus_tag="ACHKV2_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO6938986.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(61893..62708)
                     /gene="metQ"
                     /locus_tag="ACHKV2_001063"
     CDS             complement(61893..62708)
                     /gene="metQ"
                     /locus_tag="ACHKV2_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO6938987.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62748..63401)
                     /gene="metI"
                     /locus_tag="ACHKV2_001064"
     CDS             complement(62748..63401)
                     /gene="metI"
                     /locus_tag="ACHKV2_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO6938988.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63394..64425)
                     /gene="metN"
                     /locus_tag="ACHKV2_001065"
     CDS             complement(63394..64425)
                     /gene="metN"
                     /locus_tag="ACHKV2_001065"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO6938989.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64613..65188
                     /gene="gmhB"
                     /locus_tag="ACHKV2_001066"
     CDS             64613..65188
                     /gene="gmhB"
                     /locus_tag="ACHKV2_001066"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO6938990.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15397 a        16248 c        18039 g        15641 t
ORIGIN      
        1 taagaaaata aaataataca gctaatacca gatggcaggt tgtagtactg ctagcgaagt
       61 gagtatttca cgtaatgtct ccccatcagg aaacacgagt tccggggcaa tctcaaacag
      121 cggccacagc ataaagccgc gattcttcat atcgtaatgc gggacggtga gtcgttcggt
      181 attaatggtt tcattaccaa acaacatgat atcaagatcg agtgtgcgcg gtccccagcg
      241 ttctgcttta cgaacgcgac cttgctgcaa ctctatttgc tgtgtgtgat taagtaattc
      301 ttcaggcgca agtgttgttt ccagcgccac ggctgcgttt aagtaatcgg gttgatcttg
      361 cggccccagc ggtggggtgc ggtaaaacga agaaacggta agaatgtggc tttcagggat
      421 atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca gattgctgcc
      481 tatggcaata tacgccactg tcatgcggta ccctcacgac gtggtgcgcg tttgcgtgga
      541 cgacgagtac gacgacgcgg tgacggttct tcatccagct cgttgagcat ccctttttgg
      601 tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgctg cagttcagcg
      661 ttacgctcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa cttaggatgc
      721 tccagcaatt tccatgcgcg tttaccctga cgtcgggaca tacgcaactg caactgccag
      781 atatcgcggg ttaacgtcgt cagacgtttc gggattgcca gtgaacggca ggcttcgtcc
      841 agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg
      901 atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacag gaacgccggg
      961 ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg ttcaatgatc
     1021 cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg gaacagcggc
     1081 tggaacagat gatattcaca caacagctta taggtttcgt aaccgtagcc cgcttgtagc
     1141 agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcagcag ggtagcgagg
     1201 cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc ggcaaaacgt
     1261 actgcgcgca gcatacgtac cggatcttca cggtagcgcg tttccgggtt accaatcaga
     1321 cggataacac cgtccttcag atccttcata ccgccaacgt aatcacggac ggtaaaatcc
     1381 gctacgctgt aatacaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg
     1441 gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg
     1501 tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca
     1561 aacatcacat gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg
     1621 cgcacctgct caggcgtggc gttagtggtt acgtcaaaat ctttcggctt tttgccgagt
     1681 aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt attgagcctg
     1741 tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc
     1801 gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt
     1861 agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa
     1921 catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg
     1981 ttgaatttac aattgccgac tcaggcactg cggtaagccg ccagtttttg acggctgact
     2041 gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt
     2101 gaagtgccgc gattagtacc gggcgtggat cgcctttcgg caacgcaggc gcatgattct
     2161 gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg taatctggca
     2221 ctttccagcc aaaaagctgg tacagcgaga tttgccgcac tgtcggttcg atcaaatctg
     2281 ccccgcggac aatttcgctc acgccctgga agtgatcatc caccaccacc gccaggttat
     2341 aggcaaacaa accatcacgg cgatgaataa taaaatcttc ccgtgccagt ttttcgtcgg
     2401 cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc tggcggatac
     2461 gcactgcggc gttgtctggt ccatgatgca acacccggca atgaccgtcg taaataccgc
     2521 caatgctttg aatacgcgca cgcgtgcagg tgcagtaata acttagtcct tgttcatgta
     2581 accaggcgag tgcttcacga taggcgtggt gacgttgcga ttgccagaga acatcgccgt
     2641 cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca ccgggaactt
     2701 cacgaggcgg gtcgatatct tcaatgcgta ccagccagcg accgtgccgg gcgcgagcct
     2761 gcaaatagct gccaagcgca gcaatcagag aaccaaaatg aagctcgccg gaaggagagg
     2821 gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga
     2881 gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt
     2941 cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca
     3001 gacggcgaat accaatttca acaccgcagg attcacagta gccgaaatct tcgtcttcca
     3061 cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca
     3121 gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg aaattggctg
     3181 cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg
     3241 cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct
     3301 tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat gttttacggt
     3361 tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg
     3421 aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac
     3481 acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca
     3541 gttacttact ttactacaat gtaaccggca gtgatttttt aagagccatg ccttcagcag
     3601 aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt
     3661 gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca
     3721 cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac tcccgaaggt
     3781 gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg
     3841 taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa
     3901 tacggctgcg ttctgcgccg tattttactt cgcttttcag cgagctatag cctgacagtt
     3961 ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa agcgtgttga
     4021 cgcaaataaa tgcgccgctc tggctttgag ttaattccca ggtgtgtggg tatttccgtt
     4081 tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa ccggtcatcg
     4141 cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta
     4201 aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagaa aattccattg
     4261 cgacaatcct tgttattgcg ttagcaccca gcgttttagc ggcgtgtagc gtgtgcgtcc
     4321 acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcatatgacc agttaaaacc
     4381 tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa
     4441 cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca tattcgccag
     4501 ctggattaag ccgcgtggcg gctgacgcat ccctaaccac accacacgcg aacgcagcca
     4561 ttggccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag
     4621 agaaagcgcc ttctctttct ctgcgctcac ttcgcctaaa aatgccagag tcagatgcaa
     4681 attatcggcg gcgaccggac gtcccgcctc aagtgggaag tgtgtggcgc gccagtggat
     4741 aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt gcgggtcaga
     4801 catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa
     4861 ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtgttacctg aattacttac
     4921 cgctctcgat tatgcgccgc aggtattatt aagtgcgccg accggggccg ggaaatcaac
     4981 ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta tcctcctgga
     5041 gccacggcgt ctggcggcgc gtaacgtcgc gcaacggctg gcggagctgc ttaacgaaaa
     5101 gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg
     5161 cctggaagta gttaccgaag gcgtgctgac gcggatgatc cagcgtgacc cggaactgag
     5221 cggtgttgga ttggtgatcc ttgatgaatt tcatgagcgt agcttgcagg cggatttggc
     5281 gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat
     5341 gtcggctacg ctggacaacg accgcttgca gcaaatgctg ccagaagcgc ccgtcgtcat
     5401 ctcagaaggg cgctcgtttc cggttgaacg ccgttattta ccgctgccca cgcatcagcg
     5461 ttttgacgat gccgttgcgg tagccaccgc tgaaatgctg cgtcaggaaa gcggatcatt
     5521 actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac tggcttcgcg
     5581 catcggcagt gatgtattgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg
     5641 aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc
     5701 tgaaaccagt ttaaccattg aaggtattcg tctggtggtg gattgtgccc aggaaagggt
     5761 ggcgcgtttt gatccgcgaa cagggcttac gcggttgatt actcagcgaa tcagtcaggc
     5821 gtcgatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatct gcctgcattt
     5881 aatcgccaaa gaacaagcag aacgcgctac cgcgcaaagt gaaccggaga tcttacaaag
     5941 cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc cggcgcagat
     6001 gagctggctg gatcaaccgc caacggtgaa tctactggcc gcgaaacgtc tgttacaaat
     6061 gctgggggcg ctggacggtg aacggcttag tgcgcaaggg caaaaaatgg cagcgctggg
     6121 taacgatccg cgtttagcgg caatgctggt tagcgcaaag agcgacgacg aagctgctac
     6181 cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg
     6241 cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg
     6301 cttaaacgta cgcggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccggggc
     6361 gtttgccgat cgcattgctc gccgccgtgg gcaagatgga cgctatcaac tggcaaacgg
     6421 catgggggcg atgcttgatg ccgacgacgc gctaagtcgc cacgaatggt tgatcgcgcc
     6481 gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgt taccggtcga
     6541 tattgatgaa ttagtacaac gctgtccgca gctggtgcag caatctgaca ccgtggaatg
     6601 ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc agttgacggt
     6661 gaaagtgcag ccgttggcga aaccgtcaga agacgagttg catcaggcga tgcttaacgg
     6721 catccgtgat aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt
     6781 gcgtttgtta tgcgccggaa agtggttgcc agaatatgac tggccagcgg ttgatgatga
     6841 aagtttgttg gcgacgctgg aaacgtggct gctgccacat atggccggtg tacattcact
     6901 acgcggcctg aaatcactcg acatttatca ggcactacgc ggattacttg attggggaat
     6961 gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat
     7021 cgccattcgt tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc aggagatgtt
     7081 tggtgaggcc actaatccga cgatcgccca ggggcgcgtg ccgctggtgc tggaattgct
     7141 ttcacccgcc caaaggccgc tgcaaatcac gcgtgatttg ggggccttct ggaaaggggc
     7201 gtatcgtgag gtgcaaaagg agatgaaagg gcgttatccc aaacacgtct ggccggacga
     7261 cccggcaaac acggcaccga cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc
     7321 ctctccctgt gggagagggc cggggtgagg gcaccagcgc gcacgttcac cctcacccta
     7381 accctctccc ttaagggaga ggggaccgat cgagctcaaa ttttgagaga tatcttcttc
     7441 tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag
     7501 catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa
     7561 acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta
     7621 tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg
     7681 tggcaaacgc ggctggctat ggctgctgtt aaaactggct atcgtttttg ccgtgctgat
     7741 cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg
     7801 gcaactgcct gcggcagttt atggtcgaat ggtcaacctt gagccagaca tgaccatcag
     7861 caagaacgag atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac
     7921 ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgattcgcc gtccgtttga
     7981 tttcccggac agtaaagaag ggcaggtgcg cgcgcgtctg acctttgatg gcgatcatct
     8041 ggcgaccatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg
     8101 tctgatcacc atgatctcct caccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg
     8161 tttcccggat ttgctggtgg atactttgct ggcgacagaa gaccgccatt tctacgagca
     8221 tgatggaatc agtctctact ccatcggacg tgcggtgctg gcaaacctga ccgcaggtcg
     8281 cacggtgcag ggggcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag
     8341 cgagcgctct tattggcgta aagcgaatga agcctacatg gcgctgatca tggacgcgcg
     8401 ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg
     8461 cgacaacgaa atccgcggct tcccgctggc gagcttgtat tactttggtc gcccggtaga
     8521 agaattgagc ctcgatcagc aggcgctgtt agtcggtatg gtgaaaggag cgtccatcta
     8581 caacccgtgg cgtaacccga aactggcgct ggagcgacgt aatctggtac tgcgtctgct
     8641 gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgcgc gtccgctggg
     8701 ggttcagccg cgcggtgggg tgatctctcc tcagccagcc tttatgcaac tggtgcgtca
     8761 ggagcttcag gctaaattag gcgataaggt aaaagatctc tccggcgtga agatcttcac
     8821 tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gtattccggc
     8881 actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt
     8941 tagtggtgaa gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg
     9001 tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc
     9061 cttaagccag ccgaaaatct atcgtctgaa tacgtggatt gcggatgcgc caattgcgct
     9121 gcgtcagccg aatggccagg tctggtcacc gcagaatgat gaccgtcgtt atagcgaaag
     9181 cggcagagtg atgctggtgg atgcgttgac ccgttcgatg aacgtgccga cggtaaatct
     9241 ggggatggcg ctggggctgc ctgcggttac ggatacctgg attaaactgg gcgtaccgaa
     9301 agatcagttg catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga
     9361 agtggcgcag gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcgct
     9421 gcgttcggta atcgcggaag atggcaaagt gctgtatcag agcttcccgc aggcggaacg
     9481 cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg
     9541 cggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg
     9601 gactaccaac aataacgtag atacctggtt tgcgggcatt gacggcagca cggtgaccat
     9661 cacctgggtc ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat
     9721 gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc
     9781 agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat
     9841 gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gtgagatgca
     9901 gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca
     9961 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga ttaaggatat
    10021 gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa
    10081 ataacatccc acccaaagta ttaacccttt cttttcatct ggttgtttat taacccttca
    10141 ggaacgctca gattgcgtac cgcttgcgaa cccgccagag tttcgaatat tatcttatct
    10201 ttataataat cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc
    10261 gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac
    10321 agcggttagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
    10381 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc
    10441 ggcgcgacag tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc
    10501 tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc
    10561 gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca
    10621 cctgatcatt cgcggttttg cggcagaagg ccaaagccag aataactatc tgaatggcct
    10681 gaagttgcag ggcaacttct ataacgatgc ggtcattgat ccgtatatgc tggaacgcgc
    10741 tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggcctgtt
    10801 gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg
    10861 tactgacagc ctgttccaga ctggttttga ctttagcgat gcgctggatg atgacggcgt
    10921 ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga
    10981 agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt
    11041 caccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa
    11101 agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaacgaagg
    11161 ggcgaagaac aacacctatt ctcgtaatga gaagatggtg ggctacagct tcgatcacga
    11221 atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca
    11281 aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg
    11341 tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga
    11401 tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga
    11461 tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc
    11521 ctggtttggt tacgacgact ccgtaccgct gctcgatctg tacaatccgg tgaataccga
    11581 tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataagca
    11641 gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct
    11701 gggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgccg ggacgaccga
    11761 taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg
    11821 cgtaacacct tactttagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga
    11881 tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc
    11941 ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct
    12001 gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg
    12061 cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac
    12121 ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt
    12181 gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg
    12241 tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc
    12301 ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt
    12361 cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt
    12421 cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac
    12481 cgcaaccttc cgtttctaat ttctcttttg gggcacggat ttccgtgcct gtttcacaag
    12541 ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc
    12601 tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg
    12661 aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt
    12721 ggccgtcatc agccgccgtc ggaaggggag attcttcttg atgcccagcc gctggaaagc
    12781 tggagcagca aagcgttcgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca
    12841 gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcacta
    12901 gggcgctttg gtgcggcaga tcgcgaaaaa gttgaggaag ctatctcgct ggttggctta
    12961 aaaccgctgg cgcatcggct ggtagacagc ctctctggcg gcgaacgtca gcgggcgtgg
    13021 atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg
    13081 ttggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggagcgt
    13141 ggcctgacgg tcattgccgt gctgcacgat atcaatatgg ctgcgcgtta ctgtgattat
    13201 ctggtcgccc tgcgcggcgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc
    13261 ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttctgccgca tccggcgggt
    13321 gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac
    13381 tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg
    13441 ctattgaccc aaatcgtatt gtggcgctgg agtggctgcc ggtggaatta ctactggcgc
    13501 tcggcatcgt gccttatggc gtggcagaca ccatcaacta tcgactgtgg gtcagcgaac
    13561 caccactacc tgaatcggtg attgacgtcg gtttgcgtac agaacctaac ctcgaactgc
    13621 tgaccgaaat gaaaccgtcg tttatggtct ggtcggcggg atatggtcct tcgccagaaa
    13681 tgctggctcg tattgcgccg gggcgtggat ttaacttcag cgacggcaaa cagccgctgg
    13741 cgatggcgcg taaatcgctg acggaaatgg cggatttact taacctgcaa agcgcagcag
    13801 aaacgcattt agcgcaatat gaagacttta tccgcagcat gaacccccgc tttgtgaagc
    13861 gtggtgcgcg tccgttattg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg
    13921 gtccaaacag cttgttccag gaaattcttg atgagtacgg catcccaaat gcctggcaag
    13981 gggaaaccaa cttctggggc agtaccgccg tcagcatcga tcgtctggca gcgtataaag
    14041 acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa
    14101 cgccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc gtacccgcag
    14161 tctggtttta tggtgcaact ctctcggcaa tgcactttgt gcgcgttctg gataacgcca
    14221 ttggaggtaa agcgtgagta aacgtattgc gcttttcccg gcattattgc tggcgttgct
    14281 ggtgattgcc gctacggcgc tgacctggat gaacttctcg caggcgctgc cgcgtagcca
    14341 gtgggcgcag gctgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta
    14401 cagcttgctg ccgcgtctgg cgatttcgct gctggtgggc gcaggcctgg ggctggtggg
    14461 cgtgctgttt cagcaagtgc tgcgtaatcc gctggcggaa ccgacgacac ttggcgttgc
    14521 tacaggcgcg caactgggga ttaccgtcac tacgctctgg gcgatcccag gggcgatggc
    14581 gagtcagttt gctgcgctgg cgggcgcttg cgttgttggc ttaatcgtct ttggcgtcgc
    14641 gtgggggaaa cggctttcgc cggtaacgct gatcctcgcg gggctggtag tgagccttta
    14701 ttgtggggca atcaatcagt tactggttat cttccaccat gaccaactgc aaagcatgtt
    14761 cctgtggagc accggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc
    14821 gcagttgctg ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa cactgatggg
    14881 gcttgatgat ggcgtggcgc gcaatctcgg gctagccttg tcgctggcgc gtctggcggc
    14941 gctgtcgctg gctattgtca tcagtgcgct gctggtgaac gcggtgggga ttatcggctt
    15001 tatcgggttg ttcgcgccgt tgctggcaaa aatgctgggg gcgcggcgtc tgctgccgcg
    15061 actgatgctg gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct
    15121 ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc
    15181 gccgctgcta ctgtggctgt tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt
    15241 caacgatcgt gtcgcggctg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt
    15301 gctgctgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac
    15361 gtgggcgagc ggggcgttgc tcgaggattt gatgccctgg cgctggccgc gaattatggc
    15421 ggcgctgttt gcgggcgtca tgctggcggt ggcgggctgt attattcagc ggctgaccgg
    15481 aaatccgatg gcaagcccgg aagtgctggg gattagctct ggcgcggcgt ttggcgtggt
    15541 actgatgctg tttctggtac cgggtaatgc ttttggctgg ctgttacccg cgggaagtat
    15601 cggtgcggcg gtgacgctgt tgattattat gatcgccgcc gggcgcggtg gattttcccc
    15661 acaccgcatg ttactggcgg ggatggcgtt aagtaccgca ttcaccatgc ttttaatgat
    15721 gttgcaggcg agtggtgacc cgcgaatggc gcaagtgctg acttggatct ctggttcgac
    15781 ctacaacgcc accgatgcgc aggtctggcg caccggaatt gtgatggtga ttttgctggc
    15841 gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcgcg
    15901 ggccgtgggg gtggcgttaa cgccgacgcg aattgcgctg ctgctgttag cggcttgcct
    15961 gacggcgacc gcgacgatga ccattggacc gttgagtttt gttggtttaa tggcaccgca
    16021 tatcgcgcgg atgatgggct ttcgacggac gatgccgcac atcgtgattt cggcgctggt
    16081 gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccgttcca
    16141 gatcccggcg gggctgctgt cgacctttat cggcgcgcca tattttatct atttgttgag
    16201 aaagcagagc cgttaataaa atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc
    16261 gcaaacaccc gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccaca
    16321 gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata
    16381 tggaagaaac gcttaaaacg ctcgacgtca caggccatca catcctgata gcacgtcacg
    16441 gactcggcgt cggtaaagaa aataccgaac atgccgccaa cgtggtttac caccagcgga
    16501 attccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc
    16561 agcgtttcgt gaacgcccgg ctgcgcgact tcattcagac aggcgaaacc ggctgccatc
    16621 gcaatcgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca
    16681 tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttg
    16741 cccaggcaag tgagatccgg ttccacaccg taataatcct gtgcgccagc tagcgctacg
    16801 cggaaaccgg tcattacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgcg
    16861 cgcagacctg gcaggaagtc tggcagcggt ggaacacagt tcatattgcc tgccaccggc
    16921 tcgacgataa tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc
    16981 agatcgttat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag
    17041 tttggctggc ctaacgtgag tgcgccagag ccggctttca ccagcaggca gtcagcgtga
    17101 ccgtggtaac aaccttcaaa tttaataatt ttgtcgcgac cggtaaaacc acgggccagg
    17161 cggatggcgc tcattgtcgc ttcagtgccg gagttcacca tgcgcaccat atccatggtc
    17221 gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag
    17281 cttaaaccac gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc
    17341 agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac
    17401 agataagcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg
    17461 cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca
    17521 gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta
    17581 ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt
    17641 tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg
    17701 aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa
    17761 aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat
    17821 tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt
    17881 cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa
    17941 ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgctactgt taaccgttgc
    18001 ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc
    18061 cgaagcaggg gggtcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt
    18121 tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg
    18181 catggtgttg gggcgcgaag ggccaaccgt gcagatcggc ggtaatatcg ggcggatggt
    18241 actcgatgtt ttccgcctga aaggtgatga agctcgccat acgctgttgg caaccggtgc
    18301 ggcggcgggt ctggcggcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga
    18361 agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt
    18421 cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg
    18481 taaactttct gacgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt
    18541 tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg
    18601 tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg
    18661 tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat
    18721 cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg cgcgggtcat
    18781 taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc
    18841 gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata
    18901 tcaccttgag gcggggacgt ttgctattgc cggaatgggg gcgttactgg cggcatctat
    18961 tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact accagctcat
    19021 tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa
    19081 accgctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc
    19141 gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata
    19201 atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat
    19261 gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag
    19321 cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg
    19381 ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac
    19441 catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg
    19501 cggttccgtt gactataccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa
    19561 cgcgaaaagc acctgcggtt gcggttcttc ctttagtatc taatctgttg ttcatgtgcc
    19621 ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc
    19681 ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc
    19741 atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata
    19801 ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg
    19861 gccagaacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg
    19921 attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc
    19981 agaccaacgg cgtccagcac cggcaacatc cattttggta agtgtcttgg ctggcgcacc
    20041 agcacgatgg tcagcatgct ggttaccatt gcaacgacca gatcggtggg atctttcacc
    20101 caaaataccg ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc tacagcggta
    20161 accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg
    20221 ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa
    20281 tccccactga gcacctaaaa aggtcaggcg ctaatctacc tgtgaaagcg cattacagag
    20341 ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg
    20401 aataacggga attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg
    20461 tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg
    20521 cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg
    20581 gttttgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac
    20641 acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtattgat ccagcaggga
    20701 ttgcgcggct tgttcggcct tatccggttg cggactccag ggagccagtt gacgtaacgc
    20761 attggcaatt tgttcaatgt ttgtcgcatc gacccacatc acttttattc ccagcgaagc
    20821 cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt
    20881 cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg
    20941 cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa
    21001 ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag
    21061 tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgccagtt
    21121 tctgcactag tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat
    21181 cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg
    21241 ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg
    21301 cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc
    21361 cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg
    21421 cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt
    21481 cataaccaaa tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat
    21541 cgcccacttt cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg
    21601 gcttgcagtg ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg
    21661 atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccca
    21721 gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg
    21781 caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt
    21841 taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat
    21901 ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa
    21961 catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt
    22021 cgtcgtctgc aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg
    22081 cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa agaaatttta
    22141 agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggacct
    22201 tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt
    22261 cattttggcg aagcggcgat aaatgactgg tttcgccagc gtttgcaccc ggaagatgcc
    22321 gaaagccagc ctctgactga cgatcgctgc agcgtggcgg tactacgttt acgggacggg
    22381 gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg
    22441 aatgcacaag gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ctgggcgcag
    22501 gttggcggta ttttaaaata tacccgtcca gcgtggtggc gtggcgaaac gcctgagaca
    22561 catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg
    22621 ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa
    22681 gctgccgacg acatctccta ttgtgtggca gatcttgaag atgcggtaga gaaaagaata
    22741 tttaccgttg agcagcttta tcatcatttg cacgaggcgt ggggccagca tgagaaaggt
    22801 tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc
    22861 cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa taaactggta
    22921 ccctacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat
    22981 catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc
    23041 gctgtaaaac atgtgtttag ccatccagat gtcgagcagc ttgaattgca gggctatcgg
    23101 gtcattagcg gattattaga gatttatcgc cctttattaa gcctgtcatt atcagacttt
    23161 actgaattgg tagaaaaaga acgggtgaaa cgttccccta ttgaatctcg cttattccac
    23221 aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat
    23281 tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc
    23341 agcggtatga ccgacctcta tgcctgggat gaataccgac gtctgatggc cgtagaacaa
    23401 taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg
    23461 gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg
    23521 agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt
    23581 ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag
    23641 cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac
    23701 gtagaaggta gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc
    23761 ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag
    23821 ggtggccagg gcggtaatgg tggcggccag caacagaaat tcatggcgct gggttccggc
    23881 gtcatcattg atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg
    23941 acggtcatta aagttcaact gagcgatggc cgtaagttcg acgcgaagat ggttggcaaa
    24001 gatccgcgct ctgatatcgc gctgatccaa atccagaatc cgaaaaacct gaccgcaatt
    24061 aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg
    24121 tttggtctgg gcgagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg
    24181 aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc
    24241 ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctagca
    24301 ccggacggcg gtaacatcgg tatcggtttt gctatcccga gtaacatggt gaaaaacctg
    24361 acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat tatggggact
    24421 gagctgaact ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta
    24481 agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc
    24541 acctcactga acggtaagcc gatcagcagc tttgccgcac tgcgtgctca agtgggtact
    24601 atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg
    24661 aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc
    24721 attgaaggcg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg
    24781 aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg
    24841 aaccagcagg ctgtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct
    24901 gtgctggcac tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct
    24961 ccctcaaccc cttcctgaaa acgggaaggg ggtctcctta caatctgtga acttcaccac
    25021 aactccatac atcttcatca tcctttaggc atttgcacaa tgccgtacgt tacgtacttc
    25081 cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat
    25141 ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga
    25201 tgcccgtggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg
    25261 tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg tggcacgtca
    25321 tctgcacggt gtgcggcaag ggattaatct accgttacgg ctggaaggtg aaattgtcgg
    25381 cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat
    25441 gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc aggatagccg
    25501 tttgcgggag gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttac
    25561 tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtggtgg ctattgttga
    25621 ggtcgacagc ggtcagcttg gtgtggacag cgcaatggcg gagttacaac aactgcaaaa
    25681 cgcgctgact acgcccgagc gtaataatct ggtggcgatt gtctcgctaa ccgaaatggt
    25741 ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gtagaagatc atcgtaagcg
    25801 agttgaacaa ctgattaccc gcatgaaaga gtacggccag ctgcgttttc gcgtttcact
    25861 gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac
    25921 gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat
    25981 gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaacgaac tggcgcgacc
    26041 gctggcgcgg ctgaaagcga tggacaataa cggcttgctg cgacgaacgc tggcggcgtg
    26101 gtttcgtcac aatgtgcaac cgctggcaac gtcaaaggcg ttgtttattc atcgtaatac
    26161 cctggagtat cggcttaatc gtatatcgga actgaccggg cttgatttgg gcaattttga
    26221 tgacaggttg ctgctgtatg tggcgttaca actggatgaa gagcggtagg ttatgcgtta
    26281 aggttgaggc ggcgctggct cattcccctc agcccgaagg ggagagggga atgcccgagt
    26341 taaccgtcaa ttcgggatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg
    26401 atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga
    26461 tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga
    26521 ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt
    26581 ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc
    26641 tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt
    26701 tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat
    26761 gagtcagtaa aatgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc
    26821 gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct
    26881 ttattgtggc gatattccat gggatggatt agcacattta tcttcgtttt gcgaattaca
    26941 tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg gggaggaaga atgagtagga
    27001 cgaattattc tgaaccagaa agcaatcggg cagcgtcgtg ctgcccgtgt gcatactttt
    27061 agtcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt
    27121 tcttaacgat aaccgcacag tagaggctgt atttgccatc tttcgacggc agattacctg
    27181 aaacaaccac agaccccgcc ggaacgcgac cgtagtggat gtcgccggtt tcacggtcgt
    27241 aaatacgggt gctctgacca atgtatacgc ccatggaaat gacggaacct tcttcgacaa
    27301 tcaccccttc aactacttca gagcgcgcgc cgatgaagca attatcttca atgatggttg
    27361 ggttagcctg cagcggttcc agcacaccgc cgatgcccac gccaccggaa aggtggacgt
    27421 ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt atcgaccatg gtgccttcat
    27481 caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg gcaataaacg
    27541 caccctgacg taccgccgca ggtggtacaa cgcggaagcc ttctttctgg aaacgtgctt
    27601 cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta gcggctttct gccccttcga
    27661 tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac cactgatgcg
    27721 tcacccactg accgtcaatt ttttccgcta cacgcagtgc gccggaatcc agcagggcga
    27781 tcacctgatt taccgcttcg cgggtaacgg tgtctgcatt ggctggcgtg atctcggcac
    27841 ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa ctcttttcat
    27901 atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac cgctgatgca
    27961 cttcctgctg caactcgtta ttaagtgcac gccggtcagc ggtggcaatt atgaataaat
    28021 cttctactcg ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt cccagatcgg
    28081 caaaaatttt cccgactctc gccagcagtc ctggctggtc gagcgcgata agctcaagga
    28141 acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc ggtttcaaca gtaaagtgac
    28201 gtagtttagc cggttgacga cgcggctgtg gtggctgcca gctactttgt gtcagtactt
    28261 gctccagacc aaaccgaata acctcatgac gatctgcgga tagcgggcta ccatcgggtt
    28321 ccagcacgat aaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt
    28381 gaacgcttaa gttgcgtcta tctaactcgg cacaaacagc agcaaacaag taagggcggt
    28441 ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca
    28501 gcggtttgct taaatcatgc tgtaataaat ggcgcgcatg ccaggccagt tgatttgggc
    28561 tatgacggac aaaatagttc gcgcgacagc gtgaccaaat ttggtgcagc acctcttcgt
    28621 cgatgttatc catgcgcagt agtgccagcg cctggagttg gtgatggcga acccgttcgc
    28681 gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggcg aaatagagtt
    28741 cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg caaatgtcag
    28801 ccactgtcag gcataccaga tagcgcagac gattttccgt ttgaacttct tcggcaaact
    28861 gcttgatgac ttccgggtcc tgaatatcgc gacgctgagc tgtcaccgac atcagcaagt
    28921 gctggcgaac cagccaggcg accagctgtg tttcgcgtga gttcagcccg tggagttcgg
    28981 caaaatgcac tacatcctga gcaccgagaa tagagtggtc gccgccgcgt cctttggcga
    29041 tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggtaggcgc ggccagacgt
    29101 tcacacacaa cggatggcgc tggcgcgttt cttcactggc aaaactctcc agtttcagca
    29161 tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc
    29221 cgacgatatg tgaccattgc ggcatatacg cgccgagtac gctatggcga tgcattggca
    29281 atagcccgcg ccgcaccgct ccggggtgac gtagaatgct caaaaacagt tttcgtgctt
    29341 gcggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgtaac tggcgcagtg
    29401 tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga
    29461 tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc gtaccgcgta
    29521 gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc gggaagggcg aggatggctt
    29581 catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgtc acgcggaagt
    29641 aatccttcat catccgctcg accggttcgt taccttcgcc actgtaattc agacgctggg
    29701 cgacgctaag ctggcgatcg aataacaggc gattatcgta acggctgacg accagatgca
    29761 gggcaaagcg aatacgccac aatatatgca gacattcgtt taattccgcc cgctccgctg
    29821 aggttaagaa gccaaaccca accatttcat ccagcgatgt tgcgccaaaa tgacggcggg
    29881 ccacccattg cagagtgtgg atatcgcgca agccgccagg gctacttttg atgtctggtt
    29941 caagattgta gctggtgccg tggtaacgct gatggcgctg gttctgttct tcaactttcg
    30001 ccgcgtagaa cttgtcggaa ggccagaatc cttcgctgaa aatatgtttt tgcagttcaa
    30061 gaaatagcgc aacatcgcca attaataagc gggattcgat caaattggtg gcgacggtta
    30121 aatccgataa cccttccagc atgcactctt caagcgtgcg cacactatga ccgacttcca
    30181 gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga tcgtccggga
    30241 gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc tcgccacgac
    30301 cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc gcttcaatcc
    30361 ataatcgttg caggagctgg tcgataaact cggtgcgcgc ctcaatcaac tgttctgcag
    30421 agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata tgggctttta
    30481 tcccaccgac ggttaattca tcacggggcc agacgcatgg attttgcggt tgaccgggca
    30541 gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg cgccacccat
    30601 aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatttta ttcgtcgtgc
    30661 gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca gccgttatca
    30721 gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt ttttaccgtc
    30781 cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg ctcgatggtg
    30841 aacgtcatcc caggtttcag tacgacgttg gtttcacggg agtcatagtg cagcacctgc
    30901 ggttcttcat ggaagccgcg accaataccg tgtccgcaat attcacgaac gacggagaag
    30961 ccttctgctt cgacaaattt ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt
    31021 tttaccatgc gtagcgccag gtacaggctt tcttgcgtga tacggcacag acgttcgccc
    31081 atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa accatctttg
    31141 attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttagc atcgtccgga
    31201 ataccgtggc acaccacttc attaatagaa atgcaaacgg atttcggata accgtgatag
    31261 ccgaggcagg ctgaaaccgc gtgttgttca ttaacaatgt aatcattaca gatgcgatcc
    31321 agctcgccgg tgctgacgcc cggtttaaca tatggttcga tcatctccag cacttcggca
    31381 gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat tgagatagcc
    31441 attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg
    31501 tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc actgaaggtt
    31561 ttctataata gaaaattcga cgtctgatgc tgtacacagc gccaacaatt attggtgtcc
    31621 acggcgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat acacagactg
    31681 gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca tattccgggg
    31741 tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt ttatatagag
    31801 gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt tcacttcggt
    31861 caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa
    31921 gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct ggctgaactg
    31981 aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc
    32041 gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca tcgctggctg
    32101 ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg
    32161 gaaactcagt ctcaggacgg tactttcgac aagctgacca agaaagaagc gctgatgcgc
    32221 actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat gggcggtctg
    32281 ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa agaagcaaac
    32341 aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga cggtgttgac
    32401 ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt
    32461 gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga agaaagcttc
    32521 gtagaagctg agtaataagg cttgataact cccccaaaat agttcgagtt gcagaaaggc
    32581 ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga gcgagcgaag
    32641 atgacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca ggtagtacac
    32701 gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt gcggtttaat
    32761 gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca tccctggtaa
    32821 aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca ctgactgaag
    32881 ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag
    32941 cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact
    33001 acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac gctggtttcc
    33061 aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact gacgttgaag
    33121 ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca
    33181 tcaacattcg ccgcgttgct gcgctggaag gcgacgttct gggttcttat cagcatggtg
    33241 cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt aaacacatcg
    33301 ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc ggaagacgta tccgctgaag
    33361 tggtagaaaa agaataccag gtacagctgg atatcgcgat gcagtctggt aagccgaaag
    33421 aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa gtttctctga
    33481 ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg aaagagcata
    33541 acgctgaagt gactggcttc atccgcttcg aagtgggtga aggcatcgag aaagttgaga
    33601 ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc
    33661 gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct tgtggggctg
    33721 cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt gacagtctca
    33781 ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag
    33841 tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac tggatcgtat
    33901 ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg tgattggtgg
    33961 gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga
    34021 ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg cactgcaccg
    34081 cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta
    34141 cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg
    34201 tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga
    34261 agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg atccggcgaa
    34321 agatccaacc gcaaccatgt acgagcaact gacttacagc gaagtgctgg aaaaagagct
    34381 gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac cgattcgtgt
    34441 tttcaacatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac
    34501 tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc gtaagtatcg
    34561 cgggggatct tacccgtaat atgtttaatc agggctatac ttagcacact tccactgtgt
    34621 gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat tagcgatatc
    34681 agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac ccaaatcagc
    34741 aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt ggaatattac
    34801 ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc ccgtactctg
    34861 aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat tatggcgtcc
    34921 gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct gccgccgctg
    34981 acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga acaagcgcgt
    35041 gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag tgaaagcact gttgaaagat
    35101 aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa actgactgat
    35161 gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact gatgcagttc
    35221 tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc tggcggcgtt
    35281 ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca ttctgggctc
    35341 gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg aacacttccg
    35401 cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt gcctggaatt
    35461 ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta aaacgatgct
    35521 acagcaacag ggtagccgca ccgaagtctt aagtgggcaa caagccgctt gcgatatggc
    35581 agcgcttgag gatgttgatc aggtgatggc agccattgtt ggcgctgctg ggctgttacc
    35641 tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag aatcactggt
    35701 tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat tgttaccggt
    35761 cgatagcgaa cataacgcca tttttcagag tttaccgcaa cctatccagc ataatctggg
    35821 atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt ctggtggccc
    35881 tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag cctgccgtca
    35941 tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga tgaacaaagg
    36001 tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga tggaagtgct
    36061 gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca gtgttctggc
    36121 gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat ggccgaatcg
    36181 cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga catttgccgc
    36241 accggattat gatcgttatc catgcctgaa actggcgatg gaggcgttcg aacaaggcca
    36301 ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt ttcttgcgca
    36361 acaaatccgc tttacggata tcgctgcgtt gaatttatcc gtactggaaa aaatggatat
    36421 gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgagcgcgc gtgaagtcgc
    36481 cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag agtcgcgcta
    36541 tttgttagcg tagggcttca gtgatatagt ctgcgccatc tgatcgtaag tagttggctt
    36601 tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct tcagtattcc
    36661 tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc tactcaacca
    36721 cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat ggacggcaat
    36781 ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc cggagcaaaa
    36841 tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaggcgtt aacgctgtat
    36901 gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg
    36961 tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt gcgtctgcgt
    37021 attattggcg ataccagtcg ctttaactcg cgtttgcaag aacgtattcg taaatctgaa
    37081 gcgctaacag ccgggaatac cggtctgacg ctgaatattg cggcgaacta cggtggacgt
    37141 tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa
    37201 ccagatcaga tagatgaaga gatgctaaac cagcatgtct gtatgcatga actggcccct
    37261 gtagatttag taattaggac tgggggggag catcgcatta gtaacttttt gctttggcaa
    37321 attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga tgaacaagac
    37381 tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg caccgagccc
    37441 ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat atctgctttt
    37501 gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg gttcgccatt
    37561 gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag cggttttacc
    37621 actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc gctgatgctt
    37681 tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat ctcactttgg
    37741 gcttcgctgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc aggttccgca
    37801 gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac cattgttccc
    37861 ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca ttacagtggc
    37921 gcaatatggc tgctctatgt catgatcctg gtatggggcg ctgactccgg cgcatatatg
    37981 tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg taaaacctgg
    38041 caaggcttta tcggtggact cgctactgca gcggtaatct catggggtta tggcatgtgg
    38101 gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc agcgttagcc
    38161 tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat taaggacagc
    38221 ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct gacggctgcg
    38281 gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg gaaggtaata
    38341 tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta cttatcaccg
    38401 tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct
    38461 caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc gaatatgtta
    38521 tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca gaaccggtcg
    38581 ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga gcggcgatta
    38641 ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg ctggttttta
    38701 ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat tcgatagctg
    38761 cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc gaaacgcctg
    38821 attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc accaccatta
    38881 cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta cgtcactggg
    38941 cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct cgtgggccgc
    39001 aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag gcaggtttgc
    39061 aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg
    39121 tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa aggcagggga
    39181 gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa gcgattggtt
    39241 ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt gtacgccagt
    39301 atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg atgaagctga
    39361 cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc
    39421 cgatctctat cgccaagggg gctgggatga cagcggaact cggggtagtt tattacctgc
    39481 cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg ttgcccgtac
    39541 ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caaaggcgga ccggtatccg
    39601 agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg ttaatggggc
    39661 ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac gcataataac
    39721 gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg
    39781 tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg tcgccgttgg
    39841 tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg gttaatgatg aagatatcag
    39901 taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg tccttcgtga
    39961 tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca ttactttctc
    40021 cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac ctcgaggctt ctggtgtgcg
    40081 tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc tggaagactt
    40141 ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc cgctgccgcg
    40201 caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg tcagctgaaa tccagcaaat
    40261 taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt tccaactgcg
    40321 tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa taccagaaac agaaactggc
    40381 gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc gtttcaacat
    40441 agactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca cggtgaacat
    40501 caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc ttgccgggca
    40561 ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata acggcaccaa
    40621 agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt atgcctatcc
    40681 gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat tacgcgtgaa
    40741 cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta acgatacctc
    40801 gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggc tgggaagcga
    40861 tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac
    40921 cgatacccaa cgtgttccgg gtagcccgga ccaggtagat gtagtctaca aggtgaaaga
    40981 acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg gcgtgagctt
    41041 ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg gtatcaacgg
    41101 gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt acttcaccgt
    41161 agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag atgacgccga
    41221 cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct tcccgattaa
    41281 cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt ccaacatgca
    41341 gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga gcacctctga
    41401 tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga cctataacaa
    41461 gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac
    41521 cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga cttatgtgcc
    41581 gatcgatgac gatcacaaat gggttgttct ggggcgtacc cgctggggtt atggtgatgg
    41641 tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt ccagcaccgt
    41701 gcgtggcttc cagtccaata caattggtcc gaaagcagtt tacttcccgc atcaggccag
    41761 taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga aagacctgtg
    41821 taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg agttcatcac
    41881 cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcct tcttctggga
    41941 tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat atccggacta
    42001 tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga tgtccccatt
    42061 ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag acaaggcaga
    42121 acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg
    42181 tagtgtagcg atgactttag gcgatcaata taaatcgccg ggccacgcaa agaactgcac
    42241 cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa aagtggttat tagctgcagg
    42301 tctcggttta gcactggcaa catctgctca ggcggctgac aaaattgcaa tcgtcaacat
    42361 gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt tctaacacgc tggaaaatga
    42421 gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc gatctgcagg ctaaaatgaa
    42481 aaagctgcag tccatgaaag cgggcagcga tcgcactaag ctggaaaaag acgtgatggc
    42541 tcagcgccag acttttgctc agaaagcgca ggcttttgag caggatcgcg cacgtcgttc
    42601 caatgaagaa cgcggcaaac tggttactcg tatccagact gctgtgaaat ccgttgccaa
    42661 cagccaggat atcgatctgg ttgttgatgc aaacgccgtt gcttacaaca gcagcgatgt
    42721 aaaagacatc actgccgacg tactgaaaca ggttaaataa gtaatgcctt caattcgact
    42781 ggctgattta gcgcagcagt tggatgcaga actacacggt gatggcgata tcgtcatcac
    42841 cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt acgttcatgg ttaacccaaa
    42901 ataccgtgag catttaggct tgtgccaggc gtccgcggtt gtcatgaccc aggacgatct
    42961 tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc tacctgactt acgcgcgcat
    43021 ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac attgcaccca gtgcggtgat
    43081 cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc gctaacgcgg tgattgaatc
    43141 cggcgttgaa ctgggcgata acgtgattat cggtgccggt tgcttcgtag gtaaaaacag
    43201 caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc atttaccatg agatccagat
    43261 cggtcagaat tgcctgatcc agtccggaac agtggtaggc gcagacggct ttggttatgc
    43321 caacgatcgt ggtaactggg tgaagatccc acagattggt cgcgtaatta ttggcgatcg
    43381 cgtggagatc ggtgcctgca ctaccatcga tcgcggcgcg ctggatgaca ctgttattgg
    43441 caatggcgtg atcattgata accagtgcca gattgcacat aacgtcgtga ttggcgacaa
    43501 tacggcggtt gccggtggcg tcattatggc gggcagcctg aaaattggtc gttactgcat
    43561 gatcggcgga gccagcgtaa tcaacgggca tatggaaata tgcgacaaag tgacggttac
    43621 gggcatgggt atggtgatgc gtcccatcac tgaaccaggc gtctattcct caggcattcc
    43681 gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg gtgatgaaca ttgatgacat
    43741 gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa caagactaac gttccatctt
    43801 ttgttcgcca aactttacgg cctgtctcat tcttacgatt gcggcaggcc gtgttattat
    43861 tgtcgtttct tatattttga caggaagagt atcttgacta ctaacactca tactctgcag
    43921 attgaagaga ttttagaact tctgccgcac cgtttcccgt tcttactggt ggatcgcgtg
    43981 ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa atgtctctgt caatgagcca
    44041 ttcttccagg gccatttccc tggaaaaccg attttcccgg gtgtgctgat tctggaagca
    44101 atggcacagg caacaggtat tctggcgttt aaaagcgtag gaaaactgga accgggtgag
    44161 ctgtactact tcgctggtat tgacgaagcg cgcttcaagc gcccggtcgt gcctggcgat
    44221 caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg gcctgacccg ttttaaaggg
    44281 gttgctctgg tcgacggtaa agtagtttgc gaagcaacga tgatgtgtgc tcgtagccgg
    44341 gaggcctgat acgtgattga taaatccgcc tttgtgcatc caaccgccat tgtggaagag
    44401 ggcgcgtcga ttggcgcgaa cgcacacatt ggtccttttt gtatcgttgg accccatgtc
    44461 gaaattggtg agggtaccgt actgaaatct cacgttgtcg tgaatggtca tactaaaatt
    44521 ggccgcgata atgagattta tcagttcgcc tccatcggcg aagttaacca ggatctgaaa
    44581 tatgctggcg aaccgacccg cgtggaaatc ggcgatcgta accgcattcg cgaaagcgtc
    44641 accattcatc gtggcacagt ccagggcggt ggattgacga aggtgggcag cgacaactta
    44701 ctgatgatca acgcgcacat tgcgcacgat tgtacggtag gtaaccgctg tattctcgcc
    44761 aacaacgcaa cgctggcggg tcacgtatcg gttgacgact tcgcgatcat cggcggcatg
    44821 gccgcagtcc atcagttctg catcattggt gcgcatgtga tggttggcgg ctgctccggt
    44881 gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta accacgccac gccgttcggt
    44941 gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg aggcgattac cgctatccgc
    45001 aatgcgtata agctgattta tcgtagcggt aaaacgctcg atgaagtgaa accggaaatt
    45061 gctgaactgg cggaaacata tccggaagtg aaagccttta ccgatttctt tgcacgctca
    45121 acgcgcggtc tgattcgtta atgactgaac agcgtccatt aacgattgcc ctggtcgccg
    45181 gagaaacctc cggcgatatc ctgggggccg gtttaatccg cgctctgaaa gaacgtgtgc
    45241 ccaacgcccg ctttgttggt gttgccgggc cacgaatgca ggctgaaggc tgcgaagcct
    45301 ggtacgaaat ggaagagttg gcggtgatgg gcattgttga agtgctcggt cgtctgcgtc
    45361 gcttactgca tattcgtgcc gatctgacaa agcgttttgg tgaactaaag ccagatgttt
    45421 ttgttggcat tgatgcgcct gacttcaata ttatcctcga aggtaacctt aaaaagcagg
    45481 gtatcaaaac cattcattat gtcagtccgt ccgtctgggc gtggcgacag aaacgcgttt
    45541 tcaaaatagg cagagccacc gatctggtgc tcgcatttct gcctttcgaa aaagcgtttt
    45601 atgacaaata caacgtaccg tgccgcttta tcggtcatac catggctgat gccatgccat
    45661 tagatccaga taaaaatagt gcccgtgatg tgctggggat cccttacgat gcccactgtc
    45721 tggcattgtt gccgggcagc cgtggtgcag aagtcgaaat gcttagtgcc gatttcctga
    45781 aaacggccca gcttttgcgc cagacatatc cggatctcga aatcgtggtg ccactggtga
    45841 atgccaaacg ccgcgagcag tttgaacgca tcaaagctgc agtcgcgcca gacctgtcgg
    45901 ttcatttgct ggatggaatg ggccgtgagg cgatggtcgc cagcgatgcg gcactactgg
    45961 cgtcggggac ggcagccctg gagtgtatgc tggcgaaatg cccgatggtg gtgggatatc
    46021 gcatgaagcc ttttaccttc tggttggcga agcggctggt gaaaactgat tatgtctcgc
    46081 tgccaaatct gctggcgggc agagagttag tcaaagagtt attgcaggaa gagtgtgagc
    46141 cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa tgggaaaacc agccacgcga
    46201 tgcacgatac cttccgtgaa ctgcatcagc agatccgctg caatgccgat gagcaggcgg
    46261 cacaagccgt tctggagtta gcacaatgat cgaatttgtc tatccgcaca cgcagctggt
    46321 tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc gcggtcgtca ccgctgcggt
    46381 gattctcgac ccggcgcgcc cgattgccgg gctgaatgat tccaaaaagc tgagcgaaaa
    46441 acgtcgtctg gcgctctgtg aagagatcaa agagaaagcg ttgagctgga gtctgggccg
    46501 cgcggaacct cacgaaatcg acgaactgaa tattttacat gcgaccatgc tggcgatgca
    46561 gcgagccgtc gctgggctgc atattgcgcc ggaatatgtg ttgattgatg gtaaccgctg
    46621 cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggc gatagccgcg taccggaaat
    46681 cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc gaaatggcgg cgctggatat
    46741 tgttttcccg caatatggtt ttgcccaaca caaagggtat ccaaccgctt ttcatctgga
    46801 aaaactggct gaacacggcg cgaccgaaca ccatcggcgc agctttgggc ctgtcaaacg
    46861 cgcactggga cttgcgtcct gattcttgtg tcgagattaa gtaaaccgga atctgaagat
    46921 gtctgaacca cgtttcgtac acctgcgggt gcacagcgac tactcgatga tcgatggcct
    46981 ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg ggtatgccag cactggcgat
    47041 caccgatttc accaaccttt gcggtctggt gaagttctac ggagcgggac atggcgcagg
    47101 gattaagcct atcgtcgggg cagattttaa cgtccagtgc gacctgctgg gtgatgagtt
    47161 aacccacctg acggtactgg cggcgaacaa taccggctat cagaatctga cgttgctgat
    47221 ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg atcatcgatc gcgactggct
    47281 tatcgaatta aacgaagggt tgatccttct ttccggcggg cgcatgggcg acgtcggacg
    47341 cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt gtcgcgtttt atgaagaaca
    47401 cttcccggat cgctattttc tcgagctgat ccgcaccggc aggccggatg aagaaagcta
    47461 tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg cccgtcgtgg cgactaacga
    47521 tgtgcgcttt atcgacagca gcgactttga cgcacacgaa atccgcgtcg cgatccacga
    47581 cggctttacc ctcgacgatc ctaaacgccc gcgtaactat tcgccgcagc aatatatgcg
    47641 tagcgaagag gagatgtgcg agctgtttgc cgacatccca gaagcccttg ccaacaccgt
    47701 tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt gaatacttcc tgccgcagtt
    47761 cccgaccggg gacatgagca ccgaagatta tctggtcaag cgtgcaaaag agggcctgga
    47821 agagcgtctg gcctttttat tccctgatga ggaagaacgt gttaagcgcc gcccggaata
    47881 tgacgaacgt ctggagactg aacttcaggt tatcaaccag atgggcttcc cgggctactt
    47941 cctcatcgtt atggaattta tccagtggtc gaaagataac ggcgtaccgg tagggccagg
    48001 ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg aaaatcaccg acctcgatcc
    48061 gctggaattt gacctgctgt tcgaacgttt ccttaacccg gaacgtgtct ccatgcctga
    48121 cttcgacgtt gacttctgta tggagaaacg cgatcaggtt atcgaacacg tagcggacat
    48181 gtacggtcgt gatgcggtat cgcagattat caccttcggt acgatggcgg cgaaagcggt
    48241 gatccgcgac gtaggccgcg tgctggggca tccgtacggc tttgtcgatc gtatctcgaa
    48301 actgatcccg cccgatccgg ggatgacgct ggcgaaagcg tttgaagccg agccgcagct
    48361 gccggaaatc tacgaagcgg atgaagaagt taaggcgttg atcgacatgg cgcgcaaact
    48421 ggaaggggtc acccgtaacg ccggtaagca cgccggtggg gtggttatcg cgccgaccaa
    48481 aattaccgat tttgcgccgc tttattgcga tgaagagggc aaacatccgg tcacccagtt
    48541 tgataaaagc gacgttgaat acgccggact ggtgaagttc gacttccttg gtttgcgtac
    48601 gctcaccatc atcaactggg cgctggagat gatcaacaag cggcgggcga agaatggcga
    48661 gccgccgctg gatatcgccg cgatcccgct ggatgacaag aaaagcttcg acatgctgca
    48721 acgctcggaa accacggcgg tattccagct tgaatcgcgc ggcatgaagg acctgatcaa
    48781 gcgtctgcaa cctgactgct tcgaagatat gatcgcactg gtggcactgt tccgccctgg
    48841 tccgttgcaa tcagggatgg tggataactt tatcgaccgt aaacatggtc gcgaagagat
    48901 ctcctatccg gacgtgcagt ggcagcatga aagcctgaaa ccggtactgg agccaaccta
    48961 cggcattatc ctgtatcagg aacaggtcat gcagattgcg caggtgcttt ctggttatac
    49021 cctcggtggc gcggatatgc tgcgtcgtgc gatgggtaag aaaaagccgg aagagatggc
    49081 taagcaacgt tctgtatttg ctgaaggtgc agaaaagaac ggaatcaacg ccgaactggc
    49141 gatgaaaatc ttcgacctgg tggagaaatt cgctggttac ggatttaaca aatcgcactc
    49201 tgcggcctat gctttggtgt catatcaaac attatggttg aaagcgcact atccggcaga
    49261 gtttatggcg gcggtaatga ccgccgatat ggacaacacc gagaaggtgg tgggcctggt
    49321 ggatgagtgc tggcggatgg ggctgaaaat cctgccacca gatataaact ccggtcttta
    49381 ccatttccac gtcaacgacg acggcgaaat cgtgtatggt attggcgcga tcaaaggggt
    49441 aggtgaaggt ccgattgagg cgatcatcga agcccgtaat aaaggcggct acttccgcga
    49501 actgtttgat ctctgcgccc gaaccgacac caaaaagttg aaccgtcgcg tgctggaaaa
    49561 actgatcatg tccggggcgt ttgaccgtct tgggccacat cgcgcggcgc tgatgaactc
    49621 gctgggcgat gcgttaaaag cggcagatca acacgcgaaa gcggaagcta tcggtcaggc
    49681 cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt gaacaatcct atgccagctg
    49741 ccaaccgtgg ccggagcagg tggtattaga tggggaacgt gaaacgttag gcctgtacct
    49801 gaccggacac cctatcaacc agtatttaaa agagattgag cgttatgtcg gaggcgtaag
    49861 gctgaaagac atgcacccga cagaacgtgg taaagtcatc acggctgcgg ggctcgttgt
    49921 tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc ggtatctgca cgctggatga
    49981 ccgttccggg cggctggaag tgatgttgtt tactgacgcc ctggataaat accagcattt
    50041 gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc agctttgatg acttcagcgg
    50101 tgggcttaaa atgaccgctc gcgaagtgat ggatattgac gaagcccggg aaaaatatgc
    50161 tcgcgggctt gctatctcgc tgacggacag gcaaattgat gaccagcttt taaaccgact
    50221 ccgtcagtct ctggaacccc accgctctgg gacaattcca gtacatctct actatcagag
    50281 ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg cgtgtctctc cgagcgatcg
    50341 tttattaaac gatctccgtg gcctcattgg ttcggagcag gtggaactgg agtttgacta
    50401 atacaggaat actatgagtc tgaatttcct tgattttgaa cagccgattg cagagctgga
    50461 agcgaaaatc gattctctga ctgcggttag ccgtcaggat gagaaactgg atattaacat
    50521 cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa ctgacgcgta aaatcttcgc
    50581 cgatctcggt gcatggcaga ttgcgcaact ggcacgccat ccacagcgtc cttataccct
    50641 ggattacgtt cgcctggcat ttgatgaatt tgacgaactg gctggcgacc gcgcgtatgc
    50701 agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat ggtcgtccgg tgatgatcat
    50761 tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc cgtaactttg gtatgccagc
    50821 gccagaaggt taccgcaaag cactgcgtct gatgcaaatg gctgaacgct ttaagatgcc
    50881 tatcatcacc tttatcgaca ccccgggggc ttatccgggc gtgggcgcag aagaacgtgg
    50941 tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct cgcctcggcg taccggtagt
    51001 ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg gcgattggcg tgggcgataa
    51061 agtgaatatg ctgcaataca gcacctattc cgttatctcg ccggaaggtt gtgcgtccat
    51121 tctgtggaag agcgccgaca aagcgccgct ggcggctgaa gcgatgggta tcatcgctcc
    51181 gcgtctgaaa gagctgaaac tgatcgactc catcatcccg gaaccgctgg gcggcgctca
    51241 ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa ctgctggcgg atctggccga
    51301 tctcgacgtg ttaagcactg aagatttaaa aaatcgtcgt tatcagcgcc tgatgagcta
    51361 cggttacgcg taattcgcaa aagttctgaa aaagggtcac ttcggtggcc cttttttatc
    51421 gccacggttt gagcaggcta tgattaagga aggattttcc aggaggaaca catgaacatc
    51481 attgccatta tgggaccgca tggcgtcttt tataaagatg agcccatcaa agaactggag
    51541 tcggcgctgg tggcgcaagg ctttcagatt atctggccac aaaacagcgt tgatttgctg
    51601 aaatttatcg aacataaccc tcgaatttgc ggcgtgattt ttgactggga tgaatacagt
    51661 ctcgatttat gtagcgatat caatcagctt aatgaatatc tcccgcttta tgccttcatc
    51721 aacacccact cgacgatgga tgtcagcgtg caggatatgc ggatggcgct ctgttttttt
    51781 gaatatgcgc tggggcaggc ggaagatatc gccattcgta tgcgtcagta caccgacgaa
    51841 tatcttgata acattacccc accgttcacg aaagccttgt ttacctacgt caaagagcgg
    51901 aagtacacct tttgtacgcc gggtcatatg ggcggcaccg catatcaaaa aagcccggtt
    51961 ggctgtctgt tttatgactt tttcggcggg aataccctta aggctgatgt ctctatttcg
    52021 gtcaccgagc ttggttcgtt gctcgaccac accgggccac acctggaagc ggaagagtac
    52081 atcgcgcgga ctttcggcgc ggaacagagt tatatcgtta ccaacggaac atcgacgtcg
    52141 aacaaaattg tgggtatgta cgccgcgcca tccggcagta cgctgttgat tgaccgcaat
    52201 tgtcataaat cgctggcgca tctgttgatg atgaacgatg tagtgccagt ctggctgaaa
    52261 ccgacgcgta atgcgttggg gattctgggt gggatcccgc gccgtgaatt tactcgcgac
    52321 agcatcgaag agaaagtcgc tgccaccacg caggcacaat ggccggttca tgcggtgatt
    52381 accaactcaa cctatgatgg cttgctctac aacaccgact ggatcaaaca gacgctggat
    52441 gtcccgtcga tccacttcga ttccgcctgg gtgccgtaca cccattttca tccgatctac
    52501 caggggaaaa gtggtatgag cggcgagcgt gttgcgggaa aagtgatctt cgaaacgcaa
    52561 tcgacccaca aaatgctggc ggcgttatcg caggcttcgc tgatccacat taaaggtgag
    52621 tatgacgaag aggcgtttaa cgaagccttt atgatgcata ccaccacctc gcccagttat
    52681 cccattgttg cttcggttga gacggcagca gcgatgctgc gtggtaatcc gggcaaacgg
    52741 ctgattaacc gttcagtaga acgagctctg cattttcgca aagaggtcca gcggctgcgg
    52801 gaagagtctg acggttggtt tttcgatatc tggcaaccgc cgcaggtgga tgaagccgaa
    52861 tgctggcccg ttgcgcctgg cgaacaatgg cacggcttta acgatgcgga tgccgatcat
    52921 atgttcctcg atccggttaa agtcactatt ctgacgccgg ggatggacga gcagggcaat
    52981 atgagcgagg aggggatccc ggcggcgctg gtggcgaagt tcctcgatga gcgtgggatc
    53041 gtggtagaga aaaccggccc atataacctg ctgtttctct ttagtattgg catcgataaa
    53101 accaaagcaa tgggattatt gcgtgggttg acggaattta aacgctctta cgatctcaat
    53161 ctgcgaatca aaaatatgct tccggatctg tacgcagaag atcccgattt ctaccgcaat
    53221 atgcgtattc aggatctggc gcaagggatc cataagctga ttcgtaaaca cgatcttccc
    53281 ggtttgatgt tgcgggcatt cgatactttg ccggagatga tcatgacgcc acatcaggca
    53341 tggcagagac agattaaagg tgaagtagaa accattgcgc tggaacaact ggtcggtaga
    53401 gtatcggcaa atatgatcct gccttatcca ccgggcgtac cgctgttgat gcctggagaa
    53461 atgctgacca aagagagccg cacagtactc gattttctac tgatgctttg ttccgtaggg
    53521 caacattacc ccggttttga aacggatatt cacggtgcga aacaggacga agacggcgtt
    53581 taccgcgtac gagtcctaaa aatggcggga taacttgcca gagcggcttc cgggcgagta
    53641 acgtgctgtt aacaaataaa ggagacgtta tgctgggttt aaaacaggtt caccatattg
    53701 cgattattgc gacggattat gcggtgagca aagctttcta ctgcgatatt cttggtttca
    53761 cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg gaaaggggat ttggcgctta
    53821 atgggcaata tgtgattgag cttttctcat ttccgttccc gccggagcgc cccagccgac
    53881 cggaagcttg cggcctgcgt catctggctt ttagcgttga tgacatcgat gcggcagtgg
    53941 cgcaccttga aagccataac gtgaagtgtg aagccatccg tgtcgatcca tacacgcaaa
    54001 aacgcttcac cttctttaac gatccggacg ggctgccgtt ggaactgtat gagcagtaag
    54061 gcttgtcatc gccgcatttg cccggtaacg tgccgggcat tgctactgta aaatcgcacc
    54121 atcatgacac tcacgctcaa tagacaactt ctcacctcac gccagattct ggtggccttt
    54181 agcggcgggc ttgactccac cgttctgctg catcagttgg tgcagtggcg gacggaaaat
    54241 ccggggggcg ctctgcgcgc tattcatgtg catcacggtt taagtgccaa tgccgatgcc
    54301 tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg tgccgctggt ggtcgaacgc
    54361 gtacaacttg cgcaagaggg gctggggatt gaggcccagg cgcggcaggc gcgttatcag
    54421 gcatttgccc gcaccttgtt gcccggtgaa gtgctggtca ccgcgcaaca tctcgacgat
    54481 caatgtgaaa cttttctgct ggcgctaaaa cgcggtagcg gccctgccgg gctttcggct
    54541 atggcggaag tttcggagtt tgccggaaca cggcttatcc gcccgttgct tgcccgcacg
    54601 cggggggaac tggcgcagtg ggcattggcg catggtttac gctggattga agacgaaagt
    54661 aatcaggacg actcatacga tcgtaacttt ctgcgcctgc gcgtagtgcc gttattgcag
    54721 cagcgttggc cgcattttgc cgaaacaacg gcccgcagcg ccgcactttg tgctgaacaa
    54781 gagagcctgc tggatgaact gctggcagat gatttagcac actgtcaatc gccgcagggg
    54841 acgctgcaga ttgtgccaat gctggcgatg agtgatgccc gccgcgcggc gattatccgc
    54901 cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg acgcgttggt gaggatctgg
    54961 caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt tacgtttggg cgcgtttgaa
    55021 atccgacgct atcagtcgca actgtggtgg attaaatccg tcaccgggca aagcgaaaac
    55081 attgtgccgt ggcagacatg gcttcaaccg ctggaattac cggcggggca gggaagtgta
    55141 cagcttaatg cgggaggcga tattcgccct ccgcgtgcag acgaagcggt cagcgtgcgt
    55201 ttcaaagcgc caggattgct gcatattgtc gggcgtaacg gcggacgtaa gctaaagaaa
    55261 atctggcaag agctgggcgt gccgccgtgg ctacgtgaca ccacgccact gctgttttat
    55321 ggcgaaacgc tgattgcggc ggcaggggga tttgtgacgc aagaaggtgt ggctgaaggt
    55381 gagaatggca taagttttgt ctggcagcga aacgcttagt ttagtgaagg ccggatatga
    55441 cgcattaagc gtcgcatccg gcgaaagtca atcaggactc gctcaccacc accgtaccga
    55501 tttccgggtg gctaaagctg gtaattttat ccagacgtag ctcacgggtt tcgccagcgg
    55561 cctcgacgac cagatactcc acatttttgc gggagactaa atcgctggct ttggcctgca
    55621 atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag gcgagctcaa
    55681 gattatcgta atcatcacaa ttgattggtt gatacgtatc attcattgac ataatcgctc
    55741 accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga aagggcgtcg
    55801 tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg aacgtatcct
    55861 aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata tttttccaca
    55921 tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta cgaagataaa
    55981 ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc cgcaactgca
    56041 cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg cttaaagcga
    56101 caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt ctcgatgcct
    56161 ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg cgcctgctgg
    56221 cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt gccgatgatg
    56281 agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt gagctggggc
    56341 tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg gcgctgttta
    56401 cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa tgtgcgcagt
    56461 ttgccaatct ttccgtctgg tatctggacg acgagcagct ggcgaaagtg agcgcctttg
    56521 ccgatcgtac catgacgctg caggcaacga ttcaggatgg cgtgatctgg ttatcggatg
    56581 ataagaataa tctggaagtg aacttaaccg cctggcaaca accttcatga ttgtgatttc
    56641 ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg cgcagggcgc
    56701 gggcgggcag catgttaata agacctcaac ggccattcat ctgcgttttg acattcgggc
    56761 atccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc atttgatcag
    56821 cagtgatggc gtaattgtca ttaaggcaca ggaataccgc agtcaggaac tgaaccgcga
    56881 agcggcgctg gcccggctgg tggcagtgat taaagattta acaacagaac aaaaagcccg
    56941 acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga aagcacaaaa
    57001 atcaagcgtg aaggcgatgc gcagcggtcg ggaataaaaa gaaggaatgg atggtgaaaa
    57061 aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatgggt tgtaataatc
    57121 gggccgaagt cgatacgctt tctccggcgc aggctgccga attgaaaccg atgccgcaaa
    57181 gttggcgcgg cgtgctgccg tgtgccgatt gcgaaggaat cgaaacctct ctgttcctcg
    57241 aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct
    57301 ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta accgacagca
    57361 aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag
    57421 gcaatccgat tgaatcgcag ttcaactata cgctggaacc ggcacaatcc agtttaccca
    57481 tgacgccgat gaccctgcgg ggcatgtatt tttatatggc tgatgcggcg accttcactg
    57541 attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag cgtggctacc
    57601 tggctgcgcg cggccacagt gaaaaaccgg tgttactgtc agtagaaggt cactttacgc
    57661 ttgaggctaa tccggatacc ggtgcgccaa ccaaagtatt agcacccgat acggcaggga
    57721 aattttaccc aaaccaggat tgcagtagtt tggggcagta acccgtcttg agacagaaac
    57781 aaacgcaaaa caggccagaa ggatatattt caacattttg aatttgcacg ttttttgtag
    57841 gccggataag gcgtttacgc cgcatccggc aatggtgctc aacgcctgat gcgacgctgg
    57901 cgcgtcttat catgcctaca acccccctca taccttaagg cggataaggc atttacttta
    57961 tcaccgggtc aaaccaacaa agcgacccgc cttgatataa atccctggct tcaggtgccc
    58021 aacatactgt aactgcgttt ctggcttaaa cgcagagaca tcaccaatac gcatatgcat
    58081 cagatcggca gggctaatcc agcccgattg cgccagcgta agcggatgcc cggctttggc
    58141 aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgaccacact
    58201 gcttaactgc gctttcgcca ggccgctgac gcactgttgg cgaaaatcct cggagaacgg
    58261 attcagtgag cacatctggc gagtcaccat aaagggaata aattcgacaa tgccgcgata
    58321 gttataaccg ctatctttga ttttattggc gaacgcggtg atttctgtgg cttgttgcgg
    58381 ggtaagatcc gggactcgta agacgaaaag tttatcacta tgcttcatcg cttttttaag
    58441 ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg ttattttcac ccagatagat
    58501 tgcaacgtga ctcacagagg aagtgctgaa aacgcggatc ccaaatgagg ttacctccag
    58561 gcttgaggag aacagcaaat cgccgggttt gagatcaggt accgttattt cttttattga
    58621 ttgttcggtg aaagagcttt gatgctggaa tttgacagcc catgttttcg cctcggcatc
    58681 tactgctgtg gctgatgagt ctggctggct gatatcaacg gtacaggccg aaagtaaaag
    58741 aaagcagggg agaagcaggc ggcagtacgc ctttggttta tccattttat acaatccatg
    58801 taaaaaaggg ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca
    58861 gatattcgac gatgtcacca gtcttaatta actgtttctc gccgttgcga cgatatttat
    58921 attcgatatc gtcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga
    58981 tcagttccat atcggcaaac atcacgcccg gacgttcttt tcggtcatcc agcagcactt
    59041 cgataccttg tgcacgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg
    59101 atttgtgcat gttcatcggc agaattgcca cctggaacgg cgcgatagcg tcaggccata
    59161 cgatgccgcg ctcgtcgtag ttctgctcaa tcgccgcagc cactacgcgc gttaccccga
    59221 taccgtagca acccatagtc aggatttggt tacggccatc ttcaccctgt acggaggctt
    59281 tcagtgcttc ggagtacttg gtacccagct ggaagatgtg accaacttcg ataccacgtt
    59341 tgatcagcag cgtaccctgg ccatccgggc ttggatcgcc agccaccacg ttacggatat
    59401 ccgcaacttc cggggtagcg acatcgcgat cccagttgat gccgaagtag tgtttaccat
    59461 cgatgttagc accagcagtg aaatcactca tcgccgcaac ggtacggtca atcaccaccg
    59521 gaatcggcat gtttaccgga cccagtgaac ccggaccggc tttaaccacg gcacgaattt
    59581 cttcttcggt cgcgaaagtc agcgggctgg caacctgcgg cagtttttct gctttaactt
    59641 cgttcagctc gtgatcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa
    59701 ccgctttaac cagcagagtc ttaaccgttt tcttaatcgg cagattgaac tgttcaacca
    59761 gttccgcgat ggttttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag
    59821 cgcgcggttc tttcggcgcg atagcttctg ccagctcaat gttcgctgca tagtcagagg
    59881 tgtcggagaa gaccacatcg tcttcaccgc tctgcgccag cacctggaat tcgtgagagg
    59941 cgctgccgcc gatagaaccg gtgtcggcct gtacggcgcg gaaatccagc cccatgcggc
    60001 tgaagatttt gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag
    60061 aagtatggaa agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac
    60121 gcggacgcac ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt
    60181 tgtaagagct aagctcgtta cgaatcaggt cagtgataac ttcttcatga gttgggccga
    60241 gtacgaacgg acgctcgcca cggtcaacaa aacgcagcag ttccggaccg tactgttccc
    60301 aacgaccact ctcttgccac aaatcggctg gctgaaccac cggcatcgac acctcgattg
    60361 caccggcgtt gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc
    60421 cggtcggcag ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca
    60481 tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt
    60541 attggctagt acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag
    60601 cctgggacaa aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc
    60661 gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg
    60721 aacgttaaaa tcatgtagcc agacggcata ggttttgccc gtttcctcac ctttacgata
    60781 ggccgggcgc gggtcctgcg ccagtacttc gcggataaac agcgttaact gcggataacg
    60841 cttctccagc gtcaaaagct gcttttcgac ctctgcggta aaactcaccg ccatctctgc
    60901 agctggcgca ctttgcgcat agctggcact ggcatcggga agcgattcgg caaaggggag
    60961 atacggtttg atatccacta ccggcgtacc atcgaccaga tccaggctgc cgagcttcag
    61021 aatcacgcag tctttatggc aaacaacctc tttcagctct accagcgaca taccaatcgg
    61081 gttggggcgg aaagtggagc gcgtagcgaa aacacccatt ctggcattac cgccaagacg
    61141 aggcggacgc acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat
    61201 ccataaatgg ctgaatgctt ccaggccgcg aacggcgtca gcctggttgt agggagcaat
    61261 gagatgtagt tcgccgttgg cgctttttac cagacctggc tggcgcggaa cggcgaattt
    61321 ttctttatag ggcgagcgaa taacacctat ttgctcgaac tggaaactgc tcatttcgcc
    61381 gtaatgttaa gcgcagaacc gatacataaa gcctgacgat agcagcctgg cgtaccgctg
    61441 gtaacttcgc agctatgcag taataccgca ttggctttca ttttcgaggc gttgatttgc
    61501 atccgcttac gcgcggttgg aatgctcggc ggagagtcct gattagaggc ctggcaagag
    61561 tcgccactga cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta
    61621 taaattcgga ccggcgtggc gcgcggcgct ttcggttttg caggctccgc tttcggctgg
    61681 ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta acatggaaca gccgcttagc
    61741 atgagtgcta ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa
    61801 acgtcaatat tgaatcagga gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa
    61861 tgatattaca aattgtggaa acagcctaaa aattaccagc ctttaacagc gccgccgtta
    61921 aacactttgt ttgctgcttc gtaaacttcg tcagactgat aagcctggac gaatttcttc
    61981 acgttctcgg cgtctttgtt atcttcacgc gttacgatca ggtttacgta cggggagtct
    62041 ttatcttcga caaagatacc gtttttcgct ggagtcaggc caatctggct ggcataggtg
    62101 gtgttaataa ctgccagagc gatttgcgcg tcgtccagag aacgcggcag ttgcggtgct
    62161 tccagttcaa caattttcag attttttggg ttctcaacaa catcaagaac ggtcggcagc
    62221 aggccaacgc catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga
    62281 ccaaggttag ttggatcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt
    62341 gatttgattt tcttggagta accagcaatc ggataaacaa aagtgttgcc tactgcgacc
    62401 agtttgtagc cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg
    62461 gcgtcgatat cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaaggttacc
    62521 agctcaacgt ccaggccata tttgtctttc gcaactttct gcgcgacttc tgcaacctgc
    62581 tgttcggcac caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg
    62641 cagcctacca gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg
    62701 aacgccatac cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg
    62761 acagcccgga cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat
    62821 accagtaccg tattcatcac cgtcgcgtta tagccgatgt agccatactg atagccaatc
    62881 tgacctaaac caccggcacc gactgcacca cccatcgcgg aataaccgac cagggtaatc
    62941 agggtgatag ttgccgcatt caccagaccc ggcaacgctt ccggtaacag cactttacgg
    63001 acgatctgca ttggcgtggc acccattgcg cgggaagctt caattaaccc ggttgggatc
    63061 tccagcagag cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga
    63121 acaattgctg cctgcaaacc aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc
    63181 cataccagca agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaacggta
    63241 cgatacagct tcgcgttagc aataatttgc cccggacgcg tgacataaag caggacgcca
    63301 accggcagac caaccacaaa cccaaaaaat ccggatacga aggtcattgc cagcgtttcc
    63361 catacgccac gaaccagcag ccacatcatc ggctcagaca taacccagta cctctacttt
    63421 tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat
    63481 ttcagtcagc atgatgccga acttaacgcc accggcgtaa tccatctgcg cgctaataat
    63541 gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc agtggggcat cgaccgattg
    63601 accggtaaac tccagacgca gcatcggcac gcagtcagta aatggctccg cttgcagacg
    63661 ttcctggtaa tcttccggga tatccagatg cagggttgac tgaataaact tctgcgccag
    63721 cggcgttttc ggatgtgaga acacttcact taccgtgtcc tgctcgatca gttcgccatt
    63781 gctgatgacc gcaacgcaat cacaaatacg cttcacaacg tccatttcgt gggtgatcaa
    63841 cagaatcgtc aaacccagac ggcggttaat gtctttcagc agttcgagaa tagaacgtgt
    63901 cgttgccggg tctagcgcgc tggtggcttc atcacacagc aatactttgg gattgctggc
    63961 taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg gaaagattcg acgggtagct
    64021 atcatgctta tctccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc
    64081 gtctttcggt gtattgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga
    64141 agagagcagg ttaaaatgct ggaaaatcat accaatctgg cggcgagctt tggtcaactc
    64201 ggattctgac agcgtggtca gttcctgtcc atcgaccagc acgctaccct cggttgggcg
    64261 ctccagcagg tttacacaac gtataagcgt actcttaccc gcgcctgagg caccgataac
    64321 gccataaatt tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg
    64381 ggtgccctgg tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc
    64441 gtcattaagt tagtcgtggc atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg
    64501 gatgttaagg catccagacg tctaaatcaa tcaggtttat gcgaagagca ctttcttgca
    64561 ggtcgaaaca tgcgatacta gcgtcacatg ccttattaag gagctataaa aggtggcgaa
    64621 gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggctatgt
    64681 ccatgagatc gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa
    64741 aaaaatgggc tttgcgctgg tggtagtcac caaccagtct ggcattgctc gcggtaaatt
    64801 taccgaagca cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgaga
    64861 tgtcgatctg gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt
    64921 tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg cttttgtcag cacgcgatta
    64981 tttgcatatt gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc
    65041 agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctattacacc
    65101 tgaagcagaa aacgcggcag attgggtgtt aaatagcctg gcagacctgc cgcaagcgat
    65161 aaaaaagcag caaaaaccgg cgcaatgatt aaaagatgag cggttgaaat aaaaatgcat
    65221 ttttccgctt gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcgaatg
    65281 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaaat
//
LOCUS       DAZFTN010000014        64954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFTN010000014 DAZFTN010000000
VERSION     DAZFTN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..295
                     /locus_tag="ACHKV2_001067"
     CDS             <1..295
                     /locus_tag="ACHKV2_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6938991.1"
                     /translation="HLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPR
                     KNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            557..2179
                     /gene="yhiJ"
                     /locus_tag="ACHKV2_001068"
     CDS             557..2179
                     /gene="yhiJ"
                     /locus_tag="ACHKV2_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6938992.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2369..2482
                     /gene="yhiY"
                     /locus_tag="ACHKV2_001069"
     CDS             2369..2482
                     /gene="yhiY"
                     /locus_tag="ACHKV2_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO6938993.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2545..3612
                     /gene="yhiI"
                     /locus_tag="ACHKV2_001070"
     CDS             2545..3612
                     /gene="yhiI"
                     /locus_tag="ACHKV2_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6938994.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3609..6344
                     /gene="rbbA"
                     /locus_tag="ACHKV2_001071"
     CDS             3609..6344
                     /gene="rbbA"
                     /locus_tag="ACHKV2_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO6938995.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6344..7468
                     /gene="yhhJ"
                     /locus_tag="ACHKV2_001072"
     CDS             6344..7468
                     /gene="yhhJ"
                     /locus_tag="ACHKV2_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6938996.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7541..7816
                     /locus_tag="ACHKV2_001073"
     CDS             7541..7816
                     /locus_tag="ACHKV2_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO6938997.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7813..8172
                     /locus_tag="ACHKV2_001074"
     CDS             7813..8172
                     /locus_tag="ACHKV2_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO6938998.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8222..9082)
                     /locus_tag="ACHKV2_001075"
     CDS             complement(8222..9082)
                     /locus_tag="ACHKV2_001075"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO6938999.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9114..9383)
                     /locus_tag="ACHKV2_001076"
     CDS             complement(9114..9383)
                     /locus_tag="ACHKV2_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO6939000.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9373..10881)
                     /locus_tag="ACHKV2_001077"
     CDS             complement(9373..10881)
                     /locus_tag="ACHKV2_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6939001.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(10874..12232)
                     /locus_tag="ACHKV2_001078"
     CDS             complement(10874..12232)
                     /locus_tag="ACHKV2_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6939002.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(12309..12590)
                     /locus_tag="ACHKV2_001079"
     CDS             complement(12309..12590)
                     /locus_tag="ACHKV2_001079"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6939003.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(12587..13060)
                     /locus_tag="ACHKV2_001080"
     CDS             complement(12587..13060)
                     /locus_tag="ACHKV2_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6939004.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13085..13831)
                     /locus_tag="ACHKV2_001081"
     CDS             complement(13085..13831)
                     /locus_tag="ACHKV2_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6939005.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(14030..14431)
                     /gene="nikR"
                     /locus_tag="ACHKV2_001082"
     CDS             complement(14030..14431)
                     /gene="nikR"
                     /locus_tag="ACHKV2_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO6939006.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14437..15243)
                     /gene="nikE"
                     /locus_tag="ACHKV2_001083"
     CDS             complement(14437..15243)
                     /gene="nikE"
                     /locus_tag="ACHKV2_001083"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO6939007.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(15240..16004)
                     /gene="nikD"
                     /locus_tag="ACHKV2_001084"
     CDS             complement(15240..16004)
                     /gene="nikD"
                     /locus_tag="ACHKV2_001084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO6939008.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(16004..16837)
                     /gene="nikC"
                     /locus_tag="ACHKV2_001085"
     CDS             complement(16004..16837)
                     /gene="nikC"
                     /locus_tag="ACHKV2_001085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO6939009.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(16834..17778)
                     /gene="nikB"
                     /locus_tag="ACHKV2_001086"
     CDS             complement(16834..17778)
                     /gene="nikB"
                     /locus_tag="ACHKV2_001086"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO6939010.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(17778..19352)
                     /gene="nikA"
                     /locus_tag="ACHKV2_001087"
     CDS             complement(17778..19352)
                     /gene="nikA"
                     /locus_tag="ACHKV2_001087"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6939011.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKIWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19463..20050)
                     /gene="acpT"
                     /locus_tag="ACHKV2_001088"
     CDS             complement(19463..20050)
                     /gene="acpT"
                     /locus_tag="ACHKV2_001088"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO6939012.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(20052..21281)
                     /locus_tag="ACHKV2_001089"
     CDS             complement(20052..21281)
                     /locus_tag="ACHKV2_001089"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO6939013.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(21278..22009)
                     /locus_tag="ACHKV2_001090"
     CDS             complement(21278..22009)
                     /locus_tag="ACHKV2_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO6939014.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(22009..22473)
                     /locus_tag="ACHKV2_001091"
     CDS             complement(22009..22473)
                     /locus_tag="ACHKV2_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO6939015.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(22470..23639)
                     /locus_tag="ACHKV2_001092"
     CDS             complement(22470..23639)
                     /locus_tag="ACHKV2_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO6939016.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(23641..24225)
                     /locus_tag="ACHKV2_001093"
     CDS             complement(23641..24225)
                     /locus_tag="ACHKV2_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO6939017.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(24222..26540)
                     /locus_tag="ACHKV2_001094"
     CDS             complement(24222..26540)
                     /locus_tag="ACHKV2_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO6939018.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(26509..27114)
                     /locus_tag="ACHKV2_001095"
     CDS             complement(26509..27114)
                     /locus_tag="ACHKV2_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO6939019.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(27111..27533)
                     /locus_tag="ACHKV2_001096"
     CDS             complement(27111..27533)
                     /locus_tag="ACHKV2_001096"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO6939020.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(27537..29213)
                     /locus_tag="ACHKV2_001097"
     CDS             complement(27537..29213)
                     /locus_tag="ACHKV2_001097"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6939021.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(29204..29557)
                     /locus_tag="ACHKV2_001098"
     CDS             complement(29204..29557)
                     /locus_tag="ACHKV2_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO6939022.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(29544..30905)
                     /locus_tag="ACHKV2_001099"
     CDS             complement(29544..30905)
                     /locus_tag="ACHKV2_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFO6939023.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(30902..31483)
                     /locus_tag="ACHKV2_001100"
     CDS             complement(30902..31483)
                     /locus_tag="ACHKV2_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939024.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(31488..31739)
                     /locus_tag="ACHKV2_001101"
     CDS             complement(31488..31739)
                     /locus_tag="ACHKV2_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6939025.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(31751..32008)
                     /locus_tag="ACHKV2_001102"
     CDS             complement(31751..32008)
                     /locus_tag="ACHKV2_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO6939026.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(31983..32804)
                     /locus_tag="ACHKV2_001103"
     CDS             complement(31983..32804)
                     /locus_tag="ACHKV2_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO6939027.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(32801..33523)
                     /locus_tag="ACHKV2_001104"
     CDS             complement(32801..33523)
                     /locus_tag="ACHKV2_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO6939028.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(33564..34622)
                     /locus_tag="ACHKV2_001105"
     CDS             complement(33564..34622)
                     /locus_tag="ACHKV2_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6939029.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(34691..35068)
                     /locus_tag="ACHKV2_001106"
     CDS             complement(34691..35068)
                     /locus_tag="ACHKV2_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6939030.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(35460..36509)
                     /gene="yhhT"
                     /locus_tag="ACHKV2_001107"
     CDS             complement(35460..36509)
                     /gene="yhhT"
                     /locus_tag="ACHKV2_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6939031.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            36641..37858
                     /gene="yhhS"
                     /locus_tag="ACHKV2_001108"
     CDS             36641..37858
                     /gene="yhhS"
                     /locus_tag="ACHKV2_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939032.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWGGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(37862..38419)
                     /gene="dcrB"
                     /locus_tag="ACHKV2_001109"
     CDS             complement(37862..38419)
                     /gene="dcrB"
                     /locus_tag="ACHKV2_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO6939033.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(38492..39157)
                     /gene="yhhQ"
                     /locus_tag="ACHKV2_001110"
     CDS             complement(38492..39157)
                     /gene="yhhQ"
                     /locus_tag="ACHKV2_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO6939034.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            39378..39623
                     /gene="tusA"
                     /locus_tag="ACHKV2_001111"
     CDS             39378..39623
                     /gene="tusA"
                     /locus_tag="ACHKV2_001111"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO6939035.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(39725..41923)
                     /gene="zntA"
                     /locus_tag="ACHKV2_001112"
     CDS             complement(39725..41923)
                     /gene="zntA"
                     /locus_tag="ACHKV2_001112"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO6939036.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41997..42623)
                     /gene="yhhN"
                     /locus_tag="ACHKV2_001113"
     CDS             complement(41997..42623)
                     /gene="yhhN"
                     /locus_tag="ACHKV2_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO6939037.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            42764..43123
                     /gene="yhhM"
                     /locus_tag="ACHKV2_001114"
     CDS             42764..43123
                     /gene="yhhM"
                     /locus_tag="ACHKV2_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO6939038.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(43126..43395)
                     /gene="yhhL"
                     /locus_tag="ACHKV2_001115"
     CDS             complement(43126..43395)
                     /gene="yhhL"
                     /locus_tag="ACHKV2_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO6939039.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(43385..43981)
                     /gene="rsmD"
                     /locus_tag="ACHKV2_001116"
     CDS             complement(43385..43981)
                     /gene="rsmD"
                     /locus_tag="ACHKV2_001116"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO6939040.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            44131..45627
                     /gene="ftsY"
                     /locus_tag="ACHKV2_001117"
     CDS             44131..45627
                     /gene="ftsY"
                     /locus_tag="ACHKV2_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO6939041.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            45630..46298
                     /gene="ftsE"
                     /locus_tag="ACHKV2_001118"
     CDS             45630..46298
                     /gene="ftsE"
                     /locus_tag="ACHKV2_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO6939042.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            46291..47349
                     /gene="ftsX"
                     /locus_tag="ACHKV2_001119"
     CDS             46291..47349
                     /gene="ftsX"
                     /locus_tag="ACHKV2_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO6939043.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            47594..48448
                     /gene="rpoH"
                     /locus_tag="ACHKV2_001120"
     CDS             47594..48448
                     /gene="rpoH"
                     /locus_tag="ACHKV2_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO6939044.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            48719..49822
                     /gene="livJ"
                     /locus_tag="ACHKV2_001121"
     CDS             48719..49822
                     /gene="livJ"
                     /locus_tag="ACHKV2_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO6939045.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            49972..50238
                     /gene="ataR"
                     /locus_tag="ACHKV2_001122"
     CDS             49972..50238
                     /gene="ataR"
                     /locus_tag="ACHKV2_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO6939046.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            50245..50772
                     /gene="ataT"
                     /locus_tag="ACHKV2_001123"
     CDS             50245..50772
                     /gene="ataT"
                     /locus_tag="ACHKV2_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO6939047.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(50769..51152)
                     /gene="panM"
                     /locus_tag="ACHKV2_001124"
     CDS             complement(50769..51152)
                     /gene="panM"
                     /locus_tag="ACHKV2_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO6939048.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            51576..52685
                     /gene="livK"
                     /locus_tag="ACHKV2_001125"
     CDS             51576..52685
                     /gene="livK"
                     /locus_tag="ACHKV2_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO6939049.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            52733..53659
                     /gene="livH"
                     /locus_tag="ACHKV2_001126"
     CDS             52733..53659
                     /gene="livH"
                     /locus_tag="ACHKV2_001126"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO6939050.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            53656..54933
                     /gene="livM"
                     /locus_tag="ACHKV2_001127"
     CDS             53656..54933
                     /gene="livM"
                     /locus_tag="ACHKV2_001127"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO6939051.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            54930..55697
                     /gene="livG"
                     /locus_tag="ACHKV2_001128"
     CDS             54930..55697
                     /gene="livG"
                     /locus_tag="ACHKV2_001128"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO6939052.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMIDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            55699..56412
                     /gene="livF"
                     /locus_tag="ACHKV2_001129"
     CDS             55699..56412
                     /gene="livF"
                     /locus_tag="ACHKV2_001129"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO6939053.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            56531..57277
                     /locus_tag="ACHKV2_001130"
     CDS             56531..57277
                     /locus_tag="ACHKV2_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6939054.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            57637..58953
                     /gene="ugpB"
                     /locus_tag="ACHKV2_001131"
     CDS             57637..58953
                     /gene="ugpB"
                     /locus_tag="ACHKV2_001131"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO6939055.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            59051..59938
                     /gene="ugpA"
                     /locus_tag="ACHKV2_001132"
     CDS             59051..59938
                     /gene="ugpA"
                     /locus_tag="ACHKV2_001132"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO6939056.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            59935..60780
                     /gene="ugpE"
                     /locus_tag="ACHKV2_001133"
     CDS             59935..60780
                     /gene="ugpE"
                     /locus_tag="ACHKV2_001133"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO6939057.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            60782..61852
                     /gene="ugpC"
                     /locus_tag="ACHKV2_001134"
     CDS             60782..61852
                     /gene="ugpC"
                     /locus_tag="ACHKV2_001134"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO6939058.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            61849..62592
                     /gene="ugpQ"
                     /locus_tag="ACHKV2_001135"
     CDS             61849..62592
                     /gene="ugpQ"
                     /locus_tag="ACHKV2_001135"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6939059.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(62579..63019)
                     /gene="yhhA"
                     /locus_tag="ACHKV2_001136"
     CDS             complement(62579..63019)
                     /gene="yhhA"
                     /locus_tag="ACHKV2_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO6939060.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            63139..64884
                     /gene="ggt"
                     /locus_tag="ACHKV2_001137"
     CDS             63139..64884
                     /gene="ggt"
                     /locus_tag="ACHKV2_001137"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO6939061.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT        15354 a        16792 c        17719 g        15089 t
ORIGIN      
        1 ccatctaatt agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa
       61 cactactgat ttcagaccag atcctgaaga tatcttcaaa tgtggacaga cgctcgaatg
      121 tggcgacccc cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca
      181 gaatcaacaa tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga
      241 aaataaaaaa ataataggtt cttcctcagg actaagttgt tttcaaccac attaaaactc
      301 tatgagtgct gtggtatatc tttttaatcc tgccagtatt tcatagtttt tcttaatcga
      361 ttgtagaggc atgtactgat gacgcctcta caatctttag ggactttctc tactatgaga
      421 taagaaattt catattatgc tttaaggctg aatgatttgt gcaaaatata attatttttc
      481 catcgaacat gaggtgtatg tcataagatt aaatgataac tatgtattcc tcgcggtgaa
      541 ttatatgagg taaaagatga aaattggaac tgtggcaggt actaacgaca gtacaacgac
      601 aatcgcaaca aatgatatgg tgcaagagca tgttactaac ttcactaaag aactgtttgg
      661 gtatattgca aatggtatag gtgatgatat atcaagtatt gccaggacaa tgctcggtga
      721 ggttctggaa aaagtagatg attggcaaat agaacggttc caacaatcta ttcaagatga
      781 caaaatttca tttaccatac aaacggatta ttcagaaaaa tatagtatgc tatcgggcat
      841 gcgtgctcat attctacgta ggaataacca ttatcagttt attgtgacga ttaatagcaa
      901 aaattacggt tgccctcttg ataataccga tattaattgg tgcggcattg tttatttact
      961 taataatatg acagtaaatg ataatgctaa tgatgttgca gttactgagt cgtacaaacc
     1021 catctggaac tggaaaatat cgcaatacaa tgtatctgat attaaattcg aaacaattat
     1081 aaaaccccag ttcgctgaca gaatctattt ctccaattgt tcacctgttg atcccacaag
     1141 taccagacca acctattttg gtgataccga tggctcagtt ggtgcagttt tgtacgcgct
     1201 ttttgcaacg ggacatttgg gaatcatggc tgaaggagaa aatttcctta gccagttgct
     1261 taatatagaa gatgaagttc tcaatgtatt gctaagggag aactttaatg agcaagttga
     1321 tactaatgtt aatacaataa tcagtatcct gaacagaaga gacattattt tagaaagtct
     1381 tcagccttat ttagttatta ataaagacgc agtgacgcca tgtacgtttt tgggtgacca
     1441 aaccggcgat cgatttagta atatctgtgg cgatcaattt atcatcgatt tgttaaaacg
     1501 cataatgagc atcaatgata acgttcatgt attagctgga aatcatgaaa cgaattgcaa
     1561 tggtaactat atgcagaact tcacgcgtat gaaaccactt gatgaggata cctacgacgg
     1621 gataaaagat tatcctgtat gtttctatga ctccaaatac aaaataatgg caaatcatca
     1681 tgggataact tttgatgatc agcgaaaacg ttacattata ggaccgataa cggtctctat
     1741 tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatta ataaaaagca
     1801 tcatactata attaatagta aaaagtttaa aactagccgg gctatctctt gtcgatcctt
     1861 taatcgttat ttttctgttt cgacagatta cagacctaaa cttgaagcac tattagcatg
     1921 ctcacaaatg ttaggtataa atcaagttgt tgcacataac ggtaatggtg gaagagaatg
     1981 cataggtgaa acggggacag ttttaggtct taacgcccgt gacagtaaac atacgggaag
     2041 aatgttcagc atgcataatt gccaaattaa ccctagtgcg gggcctgaga taaccactcc
     2101 ctggaagtct taccaacatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg
     2161 cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag
     2221 actgttgata aaacgtaata aggaatgctc tgttcctgca actgaacagg cgtgaaacgc
     2281 tatccaacag gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg
     2341 cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa
     2401 gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt tccttcttat
     2461 cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct
     2521 tacagggatt atggcgggtc gattatggat aagagtaagc gccatctggc gtggtggggt
     2581 gtcgggttac tggcggtggc tgctatcgta gcgtggtggc tgttgcgccc ggcaggtgtg
     2641 ccggaaggct ttgccgtcag caatgggcgc attgaagcga cggaagtgga tatcgccagc
     2701 aaaattgccg ggcgtatcga caccattctg gtgaaagaag gccagtttgt tcgcgaaggc
     2761 gaagtgctgg cgaagatgga tactcgcgtg ttgcaggagc agcgactgga agccatcgcg
     2821 caaatcaaag aggcacaaag cgccgttgcc gccgcgcagg ctttgctgga gcaacgacaa
     2881 agcgaaactc gcgccgcaca gtcgctggtt aatcaacgcc aggccgaact ggactccgtt
     2941 gccaaacgtc atacgcgttc ccgctcgctc gcccaacgag gggctatttc tgcgcaacag
     3001 ctggatgacg atcgcgccgc cgctgagagc gcccgagctg cgctggaatc agcaaaagcc
     3061 caggtttcgg cttctaaagc cgctatagaa gcggcacgca ccaatatcat tcaggcgcaa
     3121 acccgcgtcg aagccgcaca agccactgaa cggcgcattg ccgcagatat cgatgacagc
     3181 gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggggaagtg
     3241 ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgaccttc
     3301 ttcctgccaa ccgaacaggc gggcacgctg aaacttggcg gtgaagcccg gctgatcctc
     3361 gatgccgcgc cagatctgcg tattcctgca accatcagtt ttgtcgccag tgtcgcccag
     3421 ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa
     3481 gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac gggcttgccg
     3541 ggcgtagcgt gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct cgtggtgagg
     3601 ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg
     3661 tgagccagca ttatggaaaa accgttgcgc tgaacaatat cacactcgat attccggccc
     3721 gctgtatggt cgggctgatt ggtccggatg gcgtcgggaa gtcgagttta ttgtcattga
     3781 tttccggtgc tcgtgtcatt gagcagggca atgtgatcgt gctgggcggc gatatgcgcg
     3841 acccgaagca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca
     3901 aaaacctcta ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct cgcctgttcg
     3961 gtcacgacaa agcggagcgg gaagtgcgaa tcaatgagct gctgaccagc accgggttag
     4021 caccgtttcg cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa aaactggggc
     4081 tgtgctgtgc gttaatccac gacccggaac tgttgattct tgatgagcct acgacggggg
     4141 ttgacccgct ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga
     4201 gcaatatgag cgttctggtc gccacggcct atatggaaga ggcggaacgc ttcgactggt
     4261 tggtggcgat gaatgccgga gaagtgttgg caactggcag cgccgaagag ctgcggcagc
     4321 aaacgcaaag cgccacgctg gaagaagcat ttataaatct gttaccgcaa gcgcaacgcc
     4381 aggcgcatca ggcggtagtg atcccaccat atcaacctga aaacgcagag attgccatcg
     4441 aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc
     4501 gcattccacg cggggagatt tttggttttc ttggttcgaa cggctgcggt aaatccacca
     4561 ccatgaaaat gcttaccgga ttgctgcccg ccagcgaagg tgaggcgtgg ctgttcgggc
     4621 aaccggttga tccaaaagat atcgatactc gccgtcgggt gggctatatg tcgcaggcgt
     4681 tttcgctcta taacgagctc accgtgcggc aaaaccttga gttacatgcc cgtttgtttc
     4741 acatcccgga agcggaaatt cccgcaagag tggctgaaat gagcgagcgt tttaagctca
     4801 acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag cggctttcgc
     4861 tggcggtggc ggtgattcat cgcccggaaa tgttaatcct cgatgaacct acttctggcg
     4921 tcgatccggt ggcgagggat atgttctggc agttgatggt cgatctctcg cgccaggaca
     4981 aagtgactat cttcatctcc acccacttta tgaacgaagc ggaacgctgc gaccgtatct
     5041 ctctgatgca cgccggaaaa gtgctcgcca gcggtacacc gcaggaactg gttgagaaac
     5101 gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcaggacaga
     5161 gcaacgaagc cgaagcgcct cccgtgatac acgacaccac acacgcgccg cgccagggat
     5221 ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc gctggaactg cgacgcgatc
     5281 cggtacgttc gacgctggcg ctgatgggaa cagtgatcct gatgctgata atgggttacg
     5341 gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct tgaccgcgac cagaccgtca
     5401 gtagccaggc gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagccgctgc
     5461 ttaccagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg gtggcaattg
     5521 agatcccgcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggtgtct
     5581 ggatcgacgg tgcgatgccg agccgcgccg aaactgtaaa aggttacgtg caggccatgc
     5641 accagagctg gctacaggat gtggcgagcc gacaatccac accctccagc caaagcgggc
     5701 tgatgaatat tgagacgcgt tatcgctata acccggacgt aaaaagcctg ccagcgattg
     5761 ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gcccttagcg
     5821 tggtgcggga aaaagagcta gggtcgatta tcaaccttta cgtgaccccc accacccgta
     5881 gcgaattttt gcttggcaaa cagctgccgt acatcgcgct ggggatgctg aactttttcc
     5941 tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc
     6001 tcaccctggc ggcgctgctg tatatcatca tcgccaccgg aatggggctg ctgatctcca
     6061 cctttatgaa aagccagatt gccgccattt tcggtacggc gattatcacg ttgatcccgg
     6121 cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggccg ggacgttgga
     6181 tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaaag
     6241 cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc ataccgctgg
     6301 tgatgggctt aagcatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa
     6361 tatttttaat ctgggtatca aagagttgcg cagtctactc ggtgataaag cgatgctgac
     6421 attgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtca cgccaggatc
     6481 gttgaacctg gcaccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat
     6541 cgttaacagc ttctatcgcc cgtggttttt gccaccggag atgatcaccg ccgatgagat
     6601 ggatgccgga ctggacgccg ggcgttatac ctttgcgata aatattccgc ctaattttca
     6661 gcgtgatgtc ctcgccggac gccagccaga tattcaggtg aacgtcgatg ccacgcgcat
     6721 gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag
     6781 ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg
     6841 ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg atggcgatca tcaacaacat
     6901 taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac
     6961 ggtggaacac ttactggtga tgccgataac gccgtttgag atcatgatgg cgaagatctg
     7021 gtcgatgggg ttggtggtgc tggtggtatc gggattatca ctggtgctga tggtaaaagg
     7081 cgtgttgggc gtaccgattg aaggctcgat cccgctgttt atgctgggcg tggcgcttag
     7141 tctgtttgcc accacgtcaa tcggcatttt tatggggaca atagcgcgtt caatgccgca
     7201 actggggctg ctggtgattc tggtgctgct gccgctgcaa atgctttccg gtggttccac
     7261 gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgatcatgc cgacgacaca
     7321 ctttgttagc ctcgcgcagg ccattctcta ccgtggtgcc ggattcgaaa tcgtctggcc
     7381 gcagttcctg acgctgatgg caattggcgg cgcgtttttc accattgcgc tgctgcgatt
     7441 caggaagacg attgggacaa tggcgtaagg aagggaaact tgacttcata ctgtgatgtc
     7501 atactattga tgtcataaag tgaagtcata aggacatgcc atgagaacca aggtacaggc
     7561 tttacggaag aaacaaaaaa atacattgga tcaaatattt aaaactcctg ttcctcaagg
     7621 gatcaagtgg tctgatatag agtcactggt taaagcatta ggcggagaaa ttaaggaagg
     7681 aagaggttcg cgttgtaagc tcatactaaa tatgagcgtt gcgtgtttcc atcggcctca
     7741 tccgtcgcca gataccgata aaggcgctgt agaaagcgtg cgtgactggt tactaagtat
     7801 aggggtaaaa ccatgatcaa actgaagacg ccaaactcta tggagattgc cgggcaacct
     7861 gctgttatca cttacgtgcc agaactgaat gcatttcgtg gcaaattcct gggcttgtca
     7921 ggttattgtg attttgtctc tgacagcatt caggggctgc aaaaagaagg ggaactttcc
     7981 ctgcgagagt atcttgagga ttgtaaagct gcgggtattg agccgtatgc gcgtacagaa
     8041 aagattaaga cctttacgtt acgctatcca gaatcgctga gtgaacgcct taataatgcg
     8101 gctgcgcagc aacaagtctc tgttaatacc tatattattg aaacactgaa tgagcgtttg
     8161 aatcatttat aacttatagc ggcccaacaa tttctgttgc gccgctatat ttttgtggct
     8221 cttaagatgc ctttctaacc aaccgatgcc cctcattcat catcaggatt ttgctataca
     8281 catcttcctc gacggcgttt tttgctgatg ccatgactcg tgccagattc gcctcgttgt
     8341 gattattcac atacgctttg ccgacggcgg tgataaacgc tttgcgcaga tcgctggcga
     8401 tatttacttt agcgacgcga taattaacaa agctgcgtaa tatctccggg gcgataccgg
     8461 agccgccgtg aataaccaac ggcaccggac agacttcggc aatgcgcttc agcaggggga
     8521 tatcgatacg cggtatatcg tccaggccat gaacgttgcc aatagaaact gccagcatgt
     8581 cgcaaccggt acgctcgaca aaggtcttca ccttttccgg ttccgttttg caatcggctt
     8641 cgctgacgtg atcatcttct ttgccgagaa tcgcccccag ctccgcttca accggtacgc
     8701 cataggattt acagaaatcg acagcttcct gggtgaaagc gatattctct tcaaaagaga
     8761 atgcggcacc gtcaatcatg accgaggtaa agcccgcgcg caccgcctgt ttaacatcct
     8821 cctgggtttt cccgtgatcc agatgaagac tgaccggaac atccatttta tcggcgtgac
     8881 gtttcacgat ttcataaatg taatcgtatc ccgagagttg tgcattggtt ggcgcaatct
     8941 gaatgaagtt gggtaatccc gaacgctcaa tagcgtttaa aattgaaatt gtcgtctcca
     9001 gattggtggt attaaatgcg cccgctaata cccgtttttc ctgaatgcgg tcgagcagta
     9061 tccgtccatt aactaatggc atattgaaaa cctcatcttt taatatcagt taattattca
     9121 gcttcaccga agcggtcgcg aaataagtcg gtgagagtct gtatggcctc gttttcatca
     9181 tcaccgtcgc agcgtatttc caccgttttt ccgcctttta ttccggcgcg cataatattc
     9241 atcatgctgg tggcattaat atcattaccc tcatagtgca gcgtcacttt acagctatat
     9301 tttttggcgg ctttcgccag acaggccgcc ggacgtgcat gcaggccagt actgtttctg
     9361 acctccacgg ttttattcaa catagtgttc ctctcgatga tgtaatggca tcacgccttc
     9421 ttgttgtaat ttcaactgat accagctttt ccatttgtgc tgataacgct gtaattgatg
     9481 gtatctcgcc cgattcggta acaaacgttg ggctatagcg actggcggcg gtggaatatc
     9541 cggtgcggcg gttaataatg ctccgacaga aaccgcctcg ttaaatgcaa ttgcttcaac
     9601 cggacgctgt aaaatatccg cctttaattg cagccaaaga ggattatggg ttgccggacc
     9661 gatcactttt aacgcgctgg tgccaggcac gttaaaacag gcgaagcagt tggcaaattc
     9721 catggttaat cccagtgata cgctggcaat taacatgtcg atacttaacg tatcgccaag
     9781 cccataaaat aatccacggg tatggcgatt tttatagggc gaacccgaac cgcgaagatg
     9841 tggaataaag acgggaatgt catcgagcaa ccaattcccc gccagataat cagcatgtcc
     9901 gcgagtaagt gatgcggcga tctcctcatc ggttagccgg aacgtgttgc gcagccactc
     9961 cagcgcgaaa ccgcccacgg gcagcgacgc aaacagggtg aactggttgg caagtgagta
    10021 acagccgttt gccagcttgt cacgtttggc ctgttcatcc agcgtcggtt gtgtatccag
    10081 ttgcagaatg ccttccgtgg tccccgtcga gttaaggata tcgcctggca tcatctgcaa
    10141 cgctcgcgca cccaccatat ggtcatgtcc ggccagcgtc acgctgaccg gttgcgagaa
    10201 cccaagcgtc ttgcaaagtg actcgctgac ccatccggcg tgctcgcctg gctgcaccaa
    10261 tggcgcaaat gccgtacagg gaacgtgtaa caacgccgcc gctttcgctg accattcgcc
    10321 tttgcgcacg tccagacaca gcgtgcggct ggctaaggtg atatccgtgt gctgttcgcc
    10381 agtcagccgc cagagcagca cttccggcgc atgtagccag cgcaggccgc ggcgattacg
    10441 cagcgggtaa tgttccagca accagcgcat tttgaaggca gagtaattgc tgtgtagtgg
    10501 tagaccgcag atgtcataaa gtgccgcact gtctgcctcg ctaagcgttg ccagatactc
    10561 ttcaccgcga cggtcatacc atgccagcat tggcgtcagg atctcgccat gctcgtcaag
    10621 gaacacgcct gattcgccaa aactggcaat gctgatccgt ctgactggca gcggcgaagc
    10681 ggcgttcagt tgcgctatcg cctggcggac ctcctgccag agggcgtcga tatcgaaatc
    10741 gacatcgccc tgtggggaga tctgctttgc cgtcacgaat ttatgcgcgc ccaacgtcgt
    10801 tgcgtccagg caggaaaagc aggtgacttt gcaattggtg gtgccaatat cgataacgat
    10861 agctgcgctg ttatcaggca taaaccgtgc tccgcgtcat tcggaaaatg ccatacagta
    10921 ccagggcgat aaccggcacc agaccaatgg cgatgagatt gccgttgaag atttccagca
    10981 gccagaagcg gaacgggtta ccgccatcaa gaaaactgga aacctggccg ctggagcctt
    11041 cggcaaattt gaagttaacg tctttcgcca ttccggtaat aaacggcgcg attttggtag
    11101 caatccatag gtcggcaata atcaccggga tcgccgccag aacggtgcgg aaaatattgc
    11161 cacggctggc taaagcaatc atcgacgcca ttaccgccag gttggcaagg tcgccaagcg
    11221 gcaggacttc attacccgga aggacaaacg cgataaagac cgagattggc gttagcagca
    11281 gggcggtgga gataatcgcc ggattaccta cggcaacggc gatatccagg ccgatataga
    11341 gatcgtcacg gtcagggtat ttggcattga gatacttttt aatagcttca gacagcggta
    11401 ataaaccttc catcaggatg cgtaccatgc gtggcaggat gaacatcaca ccgccaatgc
    11461 tgatgccaag cagcaatact tttttgaaat cgtatccggc aattacgccg agcagaatgc
    11521 ccagaatagt gccaaccatc atcggttcgc cgaagatgcc aaactttttc tgtacggttt
    11581 ccgggtcgat atgaatacga ttgaggccag ggatatggtc gatgattttg tcgaccagca
    11641 gaccgaccgg gaagaacacc accgaagaga gcgtcggcaa tgagatccct tccagtccaa
    11701 agtatttttg taccagtggc gcagaccagt cggcgagttt aagtactacc accgcagcaa
    11761 tcgccgccgc cagcaaacca aagaacaggc tgccggtgct gtaataaacc aacgtaccag
    11821 caagggcaaa atgccagtag ttccacatat ctacgtcaac ggtacgggtt ttatttaacg
    11881 ccagcatcgc cacgttaatc agaatggtca ggggaataac aaacggggca attggcgaac
    11941 cccatgtaat agcggaaagc ggcggccagc ctaaatctac caccggtaaa ttaattccgg
    12001 tgcgttcaac catcgcctgg gccgctggcc cgacattgct ggtcagaata tcgaaaatgg
    12061 cataaatacc gacaaaacca atgccgactg ttaatgacga acgtaaggct ttagcgggtt
    12121 ttactttaaa acagagagca agaataaata acaccaacgg caacattacc gtcgggccaa
    12181 atcccaatat atattgcaca atattataga gggtatgcgc gatatcgttc ataataaacc
    12241 tctttttcag gcgagagcgc acctcgcgtt agcgcctgat ggagtgggtt gaaattaatt
    12301 attattaatt actttttaag gatagaaaca atttgcgcca gtactttttc ttcgccaata
    12361 ccggtaataa tcggcaagcc gttcaccacc gggacgtcga gttcataagg cactttagtt
    12421 gttgaaacta ccagtgctac gccgtcagta tttccaggta aagaggcaac gttggtttgg
    12481 gaataattga cgttaagacc ttgctctttg caatattcca tgactttttc tgttaccgcc
    12541 gtggatgtgg caatacccgt ggcgcagaca aataaaatgg tttgactcat atttcacctc
    12601 gttctttcat tttgtttaag attactgtgt gtatttctgt tgtttccatc gcaagcaggg
    12661 cttgcataaa gggcgtatcc tgaatcattt ccataatcca gcccagggca tttaattgtt
    12721 tattgctttc gcttaaggcg agcaaaaaaa tcaccctgac aggtacgggg tcaggatcgc
    12781 cgcccatatc ggcaaactct accggttccg gcaggatgcc gacggcaatt gcattgtgcc
    12841 tgacatgttt atggtccgtg tgcggaatag cgacgcacgg tggttcggta ggcagccccg
    12901 ttgggtacgt cgcctcacgt ttcagtacgt gatcggcaaa atcagggacg accagctcgg
    12961 cggcaaccaa cggttgcgct aactggcgga tgatctgttc aacgcttaac cctgccggaa
    13021 gtcgttgaat ataattttcc tgaaaatgta tgccttgcat tttattcctc ttaaaaaagt
    13081 gtacttaact cacttcccgc cgttgcagta cggtgccaag ataatattta tttcctttta
    13141 accagacgtt gccaaactca atgggtaatc ctcgggagaa aaagactaac tgctccagat
    13201 gcaaaacggg tgcgtcttca ctgatatcta aaaaatgacc acgttcatta ccaattaatc
    13261 gcgcggcata gcgactttcc gagtaacgaa tttttctttg cgacaaactt tctattgttg
    13321 gaaataaatt gtgttgatta aaatcgattt cagcaatacc ggggcatagc tcaatattga
    13381 tacggttctc tatcagcatc gctttttcat caccaattga gcgtaagcgt tcaaggtaaa
    13441 gaatatcctg accgggcgtt attcttaatt tctctgccac ataacgatta gccgattcaa
    13501 tccgtgacgt aataacttca gtggtaaaat gtattttctg gctttccagc gattccgcga
    13561 atgacaataa tccttcacct aacggatatg ccacgttctc ttttttgaca aatgttccct
    13621 taccctggac ctgtattaac acgccttctt ttaccagttg ggcgaccgct tttttaacgg
    13681 ttccccgact cacgtccagc atatccataa tctggtattc cgaaggaatg cgatcgtctt
    13741 ccaccagatc tccggtataa atactttctc ttatccactc caccacttgt tgatagagcg
    13801 gtatttgtga cgacttactt aatgatttca tggcgcgcac ctgaacattt acagttgtct
    13861 atggacaatc atggacaact tcaagcgtat agtaggtgaa cgggagaaaa agaggatcga
    13921 agcgtctcac attttgaggg accaaatcgt gatcaaaaaa atgtgacgtg agtcgatatt
    13981 tgtcagaagt ttactgcgcc gtaaggaaat aacggcgcgt tggtaggtgt taatcttcct
    14041 tcggcaggca ctgcaaatgc ccgtgccgca cgccgcgctg ggcgataacg tcatcggcaa
    14101 aatgctgcac gtcacccatg tcacctttca acacggcgat ttccagacag tcgtcgtggt
    14161 tgatgtgcac atgtagcgtg gcgacggaga ggtcgtggtg atgatgctgg gtggaaacaa
    14221 tacggctggc taagtcgcgt ttttcgtgtt catacacata cgacagcacc gcgaaacctt
    14281 gtgtgccgtg ctgctgggta gcttcctgcg ccagggcgct acgcagaata tcgcggatag
    14341 cttcggaacg gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat
    14401 cgtcatcaag cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg
    14461 gcgcacgggg aatgcgggta atatcgcgtt ttgtagcaca cgtccggcgt cagaggaaaa
    14521 ggttaatttc tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac
    14581 tcgctggcaa aagcgttcca ccaggcgtaa gtcatgagtg atgaacaggc aggcagtgcc
    14641 aaactgttgt tgtagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag
    14701 gttagaaacg gcttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag
    14761 gcagacgcgc tggagctggc cgccgcttaa ctgcggtggg cgcttgtcga gcacgctgtc
    14821 gtcgagatcg accgcgtgca gcatttcgct ggcacgcgcc agttgttcgg attttttcag
    14881 cgatagcagg tggcgcatcg gttcccgcag gatctcgcgc acggttttgc gcggattcac
    14941 ggcgctgatg gagtcctgaa ataccatctg aatatcgcgg cggaacgctt tacgctgggc
    15001 gcgattgagc tttgccaacg attcgccacg ccagctaata tttccctgtg agggcgattc
    15061 taagcctacc agtagccgcg agagagtgct ttttccgcag ccgctgcgtc ccagcagggc
    15121 gacggtttca ccgcttttca gggtcaggga aacgttattc agcaccgcct ggtgctgatg
    15181 ttttccgcta aacccaccgt gggcatagtg atgggaaagg tcggagacgt taagtaaagt
    15241 catgatgcca gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgttacc
    15301 gcatgtttgg gggcgttaaa cagcgtttcg acatcgccct gttcgacaat cttaccgtca
    15361 gacattaccg ctacgtcatc cgccagacgc gccaccacgc ccatatcatg ggtgaccagc
    15421 agcatccccg gcgcctgttt ttgcataatg ctttccagca gatcgagaat gcgtgcctgt
    15481 gctaccacgt cgaggtcggt ggttggttca tcggcgatga taaacggtga ttcgcaaagc
    15541 accgccatcg caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc
    15601 ttcagcacgc gtgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg
    15661 tcatcggcgg gtttccccag cgccaggcac gtctcccgcg cgtgggtgtg catggtgtgc
    15721 agtggattaa aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg
    15781 gcgcagggag aaaccggttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcct
    15841 gcgggcagaa ttcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg
    15901 actaacgcca gcacgcgccc gcgtttcagg gttaacgaca cgccgtgcac cagcggctgc
    15961 gcggcctgta gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg
    16021 tcaccagatg aggatccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca
    16081 cgctgataaa cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact
    16141 ggcgcgcgtc gttaatcatc acgccccatt cggcggtcgg cgcggtcacg ccgaggccga
    16201 ggaaggacat cccggcaacg tgcagcatca tatggccgat atccagcgtt gccagcacca
    16261 gcagcgaagg gatcaccgca cctgccagat gatcaacaaa cactcgcaca tggcccgcgc
    16321 cagaaagccg tgatgccagc acaaactcgc gctggcgcag tgaaatcacc aggctgcgca
    16381 ccatgcgtgc ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc
    16441 cagtgccgag cacgccaacc ataaagaatg acagaatcga ggtcgggaag gtcataaaca
    16501 tatcggcgac gcgcatggta gcctgatcaa cgcgcccgcc aatcagcccg gcgctgccgc
    16561 caataaccag ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca
    16621 cacgggtcgc tgccatcagc cgcgagaaaa tatcgcgccc taagtgatcg gtgcccaacc
    16681 agtgctgcgc atccggcgaa agcaggcgcg acggcaaatc aatcgcctgg gggtcatacg
    16741 gcagccacca ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca
    16801 ggcgtaccga ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg
    16861 aatgcgtggg tccagtgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac
    16921 caccaccatc atcagcgtaa agcactggat caccggatag tcacggttaa aaatcgccga
    16981 caccgcatag cgcccgacgc cgggccaggt aaagatgttt tcgataatca tcgtcccgcc
    17041 aatcagttcg ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag
    17101 aatatgacga cgctcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg
    17161 acgctgaccg gcgacgtcca gcatactggc gcgcagcagc cgtgcgttaa tcgccagcga
    17221 cataaaggca atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc
    17281 gggtagccat tgcagataca ccgaaaacgc catcaccagt aaaaatgcca gccagaagtt
    17341 gggcatcgac acgccgagaa acgcgatgaa acgtacggcg aaatccggca gacggtcacg
    17401 atggcgcgcc gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc
    17461 acccgccagt tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgctg
    17521 cgtggcaaat gagatgccga aatcaagatg cagcgccttc cacaaccagg tgccgtactg
    17581 cacatacagc ggctgatcca gtcccagcat ggtgcgggta gaggccagca tctccggcgt
    17641 cggcggcagg ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag
    17701 catcagaaaa atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg
    17761 taatacgtaa cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg
    17821 gcgatcggcg cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg
    17881 taactgatag gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagt
    17941 gcctgacgtt gtgcttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc
    18001 ggcttgtcgg ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa
    18061 ctcaggaagg cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa
    18121 cgaccgtcgc gctgacgcgc atagatactg ctctcttctt cgccaatcag cgagacatct
    18181 gcgccaatct ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg
    18241 gtgccgatga acgaaagttc aatgtgcagc ggctgaccat ttttctcgcg gatgtctttg
    18301 cccgcaggca gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac
    18361 tggcgcggtt tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg
    18421 acctgctggg tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta
    18481 agagcttcac gtactgccag ctcattggtg ggggctttgg cggtattaag cgccagcatc
    18541 acggtttcga tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg
    18601 aaggtatcga gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca
    18661 aacgccaccg cgcgggtagt cgggtctggg atgacgttaa aagtgatctt cttaattgcc
    18721 ggcttttcgc cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat
    18781 tcctgcaaaa cccacggtcc ggtgccaatc ggcgctttaa tcccgttcat ggtttcatgg
    18841 tttttaaact gcgagggggc gataaagcgg aaagggcgcg gcagggccag ttcttgcagg
    18901 aaaggatagt aggcgctttt cagggtgatt tgcagctctg ttttactgag tgctttaaca
    18961 tcaacaatct ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg
    19021 cggaagtttt ctgccgccgc ctctgcatca aacggttcgc cgttagagaa tttcacgtca
    19081 tcacgcaggg tgaaggtcca gattttacca tcttctgaat gagtccagct ttttgccagc
    19141 cacgggatca ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg
    19201 gcgaacatct ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag
    19261 gcggtggtga tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc
    19321 gcaaatagag tgcggcggag tgtggagagc atggtaaccc caatggatta aaatagatgg
    19381 cggaaataag tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata
    19441 gatgggcagt cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg
    19501 tgagcgtaaa gggagtaggg gtgcagatcg ccaggcttaa attttctagc tgacaatggc
    19561 tgactgacag cgaggagtga taggtgctgt ctacgctgac gatttgccag gcgctgccac
    19621 cacgctgttt aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct
    19681 gatcaggatg cacggcgtcc atctcagcat gttccccgag gctgaacacg gcgttcgcca
    19741 gccagcgcca gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat
    19801 cactcaacag cagggcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt
    19861 ccggcgcaaa tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg
    19921 tgtgcgaaag caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt
    19981 gctcgcgtaa acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta
    20041 tccgatacat atcagggcca acgtttaatg atgatggatg tattaatgcc gccaaaggca
    20101 aagttgttac tctgcaaaaa ctcacaatca accttgcggg cttcatgcat gatgtaatcc
    20161 agcgcgccac aattcgggtc tggtttattc aggtttagcg taggcgcaaa ccaaccttcg
    20221 cgcatcattt gcagactcat ccaggcttcg agcgcgccac aggcaccaag cgtatgacca
    20281 aaataacttt tcagcgagga gagcggcaca ttatcaccgt aaatggttgc tgtcgccaga
    20341 ctttctgcca tatcaccgcg atcggtcgcc gtaccatgcg ctgaaatata gccgatatct
    20401 tgtgcgctta acccggcgat tttcagcgat tgctccatac aatactgcat ggtttcgcgc
    20461 tgcggctggg taatatgtgc cgcgtcgcag ttagtcgcaa agccgacgat ttcaccgtaa
    20521 atggtcgcgc cgcgcgcttt ggcgtgctcc agttcttcga gaatgagcgt acctgcgcct
    20581 tcaccaatca ccagcccgtc gcggttttca tcgaacggtg aaggtgtggt tttcggcgca
    20641 tcattgtgct ggctggtggc gaaaagcgta tcaaataccg ctgcttctga aggacataac
    20701 tcttcggcac caccagcaac catcaccgtt tgataaccgt ggcgaatggc ttcccacgca
    20761 taaccgattg cctggctgcc ggaagtacag gcgctggagg ttgggatcac ccgcccgcgc
    20821 aggccgaaaa agaggccagt attaacggcg gtggtgtgcg gcatcatctg cacataggtg
    20881 gtgccagtga tgttattggt gtgcttttcc gtcagcatgg tggcgaactc gcttaccggg
    20941 ccggtactgc cggtcgatga accgtacgca atgcccgttt cgccgctggt taatatcgga
    21001 tcgccaatca accctgcctg ctctaacgcc agctcgctgg cacgggtcga catttgcgac
    21061 acgcggccca tcgcacggat acgcttgcgg gtgtagtgct ccggtagcgt gaaatcatca
    21121 acaggcgcgc caagcagggt gtgcagccca tcgtagacct gccactccgg cattttgcgc
    21181 accgcatttt catatgccag taagcgtgca gaaacgtcct gccagttttc accaaaggca
    21241 gtcacgccgc ccattcctgt aatcactacg cgacgtgtca taacatccct ccgttgatgg
    21301 aaataacctg gcgggtgacg taacccgcaa tatcagacat taaatagctg gcaagcccgg
    21361 caacttcttc cgcctggccc atgcgtttca tggggatcat cgacattgcc tctttcaggg
    21421 cgctctcttc catttcgatc atcccggtat caataagccc cggcgcgatg cagttgacgg
    21481 tgattttgcg cttcgcgagt tcgatagcca gcgcttttgt tgcgccgata attccggctt
    21541 tggcagcgct gtaattcacc tggccgcgat tgcccattac gccggaaacc gaggatagcg
    21601 tgatgatgcg cccaccctgg cgtgcgccga tcatcggcat aatgcacggc tgaatgacgt
    21661 tgtagaaact gtcgaggttg gtgtggatca ccgcatccca gtcgtcatcg cttaaagccg
    21721 gaaaagcggc atcgcgggcg atcccggcgt tactgaccac gccgtaccag gcaccgtgtt
    21781 gcgcgatttc gtgctccagc acttcccgac actgttcgcg gtttgcgacg tcgaaactga
    21841 gcaaacgccc gttaccccca ttggcgacaa tggcattcag cgtttcctga gcgcctgtgg
    21901 cgtcacggtg atagtgaacg ccaatgttaa agccgtctgc cgccagttgg caggcgatgg
    21961 cgcgaccaat gcctttgctg gcaccagtaa ccagaactga acgactcatc aggacgctcc
    22021 ttgttgaaac agggtggtaa gttcttccgc ggtcggctgg aaggtgttaa tgcggcccgt
    22081 cgcctcgccg tcgttgatgc tgcactcaaa actgccgaag cgatcgtctt gcatcagcag
    22141 tttgacggta atggtcagtg tctggcctgc gggcagaatg ccagtggtgc aaagcaattc
    22201 acgcgcgccc agtatcatcc ctaactcaat actgtttttg ccctgctggt ggcgatgcca
    22261 gccagaccag acgccgacgg tttgcgccat cagttcaagg gtaaaccagc cggggagatt
    22321 gccgtccggg tcgagaaacg gtgccagtac gccactgggc gaaaccgtca cgcggcacac
    22381 cgcgctgtca tcgctgacgc tcaccacgtc ttccagcagt aacatgggcg cgtcgtgggg
    22441 cagataagcg ccgggggata aatagtggct cacgaaaccc tcccgagcag aatgctggcg
    22501 ttattgccgc caaaggcgaa ggaattggac aggatcaccg gacgtgccag cggctgcggt
    22561 ttttcgataa tgccacacgg tggcagcgtg ggatcgcgcg gcgacaggct gaagtcctga
    22621 gcgggcagcg gtaaatcacg ctgtaaaatc aacatactaa ttgccgcctc ggtaatgccc
    22681 gctgtgccca gcgtgtgtcc ggtcaaatgt ttggtggagc tgcacggtac gcgttcgcca
    22741 aacagggcgt taaccaccat tgattcaatc tgatcgttga gttgtgtggc ggtgccgtgc
    22801 aggttgatat agccgacgtc atctggcgta agctgcgcat cggtcagcgc ctgattaata
    22861 gcgcggatcg ctccttcgcc ctgcggatgc ggggctgaaa tatggtaagc gtcgctcgat
    22921 tcaccaacgc ccagcaacgc gatgggctgc ggttcccggg tcagcagcat tagccccgcc
    22981 ccttcaccaa tggtgatacc tgcgcggtca cgaccaaacg gctgacataa ggttggcgac
    23041 agcgattcga ggctgtgaaa accgttaatc ggcatccggc tcagggtgtc tgcgccaccc
    23101 acaatggcgg catcgaccag ccccgcttcg ataagcctgc gcccgctgat tatcgctctg
    23161 gcgctggaag agcaggcggt tgaaagggta taggccgggc cgtcgagcgc cagccagtgg
    23221 ctgagaaaac gcgacggatc gccgagttcc tgttgcgcgt agtgccatgt ggtgctcggc
    23281 tgcccgtggg tgcgcaggtt aacgtgcgtg tcgccttcat gcaggccgga ggtgctggta
    23341 ccaagcacaa tcgcaatgcg ctcgcggcca tatttcgcaa tggcgtcatc aacctgcggc
    23401 tggatctgcg ccagcgccgc cagcaggatc tgattgttgc gcgagcggtg cgcagcgaat
    23461 ttttccggga taagcggaag ctcgccatcc acgccagcca ggaccgcctg cggatgcccc
    23521 tgcaaccagc ctgcgcgcgg gcgcatgccg ggagcaacac cgcgagtcag gttggttgcg
    23581 atttcgtcaa ggttatttcc cagggcgttg atcatgccga cggcggaaat ataaatcata
    23641 tcagtcacct aagtattgaa tggtgatgtg gtatttaaag acatgctgct caatgctgat
    23701 tggcacgcgt ttgcccttgc gattcagata ggtgatttcc gtgaccagtt tgccgctggc
    23761 gttacgcagc tcgcgtttgt caccgttgtc gcgaagtgtc catcctgcgg gaagttgcgg
    23821 ttgccaggcg ctaatcggcc agtggctgag catcacgtca gccagtacct gacttgcggg
    23881 cggtaactgt gggacgacga tggattgctc ggcgcgtagc ccttgcgcat cgtaggtcac
    23941 cagaaacagg cgaatgccga ccgacgacag cccggcaagg gtgattttct gatcgtcggc
    24001 gttaagcatc actaacagcg actgggtttt gccgttgaag ctgccggtca acagttgctg
    24061 cgaattgact gcgggtgaaa tccctggtgc cggcagcgtg atgcgcgtgc caggttgcag
    24121 ccacgcctgc gggcggcctt cctgttctgg ttgcgagtgg ctacagccgg tgaggataag
    24181 cgtagcggtc agggcgaacg ctcgccagaa tgtggatttg atcatttttt tgttctcttt
    24241 ttatcgggca tagccagcgg cgaaagcagg aaggcggtga aaataccgct caccagcaca
    24301 atgccaaaac tgctgatggc ctgggtggcg ctgaatacca gcatacccag cgtcagcaag
    24361 gtggtgagca ttgccagcgc gatcgccagt agcgaagtta acggtgtacc gcgcggatta
    24421 ctgaaaaaca gcgtgtagtt gataccgatg ccaagcacca gcaccagcgc cagcagcgaa
    24481 aagagattca ccgcctgccc gctcatcgcc agcaccgcca gaccacagcc cagcgaaagc
    24541 accgaaggca ccaggctgat aagccctttg cgccagccga gacgggccac tgcgccgcag
    24601 gcaatcactg ccagcgccac cagcaacaag ccggttaaga cgtagcggta aagtgcgaac
    24661 aattcatcaa aggtgctttt gcgatcaacc caggcaatgc cgcaagggta atatgtggcg
    24721 atttcctgca tcaacgcgct acttttaacc ccttcaaccg gcaccagtac gccgctttcg
    24781 ccgttttcca gcgtcagcca cagcagacgc cagccttcac tggcggggct tgccagccat
    24841 tcatcaacgt tcactggcat ggcgttgaga tccgggttca ctgccgtcag cccggcattt
    24901 tgcagcgctt ttgttactgt cggggccgcc gttttcagca gttgtaaatc ttcctcctgc
    24961 cgcgcaaggg agttcagcgg aatggtgcgg tagttgctga taagcccctc ttttttcgca
    25021 tactcaagtg aggcggtata tttctccagt cgccgcaatg tttgctgtgg cgaatcgccg
    25081 taaaccacaa accatttttg atcgacgctc tgcccggtca gggcggtaat ggctttttcc
    25141 tgcgccagaa tatgctgcgg tagcgcctgt aactgcgaga tatcgtcatc gacgcgtagc
    25201 attgacatcc ccgccagcga aaacagcgcc agcgcgacgg gcagacccag cgacagtttt
    25261 ttattacgcc gccacgctgc cagccagcgt agcatcagcg ccatcgccgg aaccggacgc
    25321 accggcaggc cacggcacag ccacggatgc cagaacagga cggtcagaca ggaggcgctc
    25381 aacccgacgg cggcaaaaat cgccatctgg cgaatgccgg ggaagggggc gagcatcata
    25441 atcagatacg ccgccacggt ggtgagcagc gccagtagca aggcattgcg tactttcgcc
    25501 aggctttgcc acggcgaaac gtcgttgccg tgaaccatcc gctcggtgag ataatagagc
    25561 gtgtagtcag cggaaatgcc gataacgctc atgctcatca ccagcgtcat caggtgtaat
    25621 tcaccgaaaa tcaataaagt ggcgaccgtt cccgccagcg cgccgatgcc gatggaaatc
    25681 acgcaaagca gcaacgggcg taaagagcgg aacaccgcca caatcagcaa tatcaccccc
    25741 agcagcgtag ccacgcccag ggtggagata tcctgcttcg cctgttggct ggcgtaatcg
    25801 ctgtaaaaca ccgtgccgcg cgagagcaac tgtgcctgcg ggtaacgcgt tttcagatcc
    25861 ttttccagcg tattcagggt cgtgatcagc tggtgggttt gctgcatatc aaacgacgat
    25921 cccgccagtt cgccgtgcag cagataccag tagttgccct ggggatcctg cgtcaccagc
    25981 caaccgtcca tcagccgcaa acgctggccg tttttcgcca ttgccagctg cgagccgcgc
    26041 attaacatca gcggatcgtt ttgcagctcc ttgccgctta cgccggagaa tgcggaataa
    26101 agctgggaga ggatccactg tgcctgcgct tcgccgccgt tttgcaggcg ggcgcgggtg
    26161 ttggggtcaa tcaggccgtt gcgatgctgc cagaaaaacg ctccccacgc ctgctggctg
    26221 gcggcatcca ttggcccttt aacgtcgccg agcgcagcgg atttttgcag cagcgtcagc
    26281 cactcctgag cgaccaaagg attagcctct ttaccggggc tgaccagcca caccagttgg
    26341 cggtcaagac gctgcataaa gccgtcattc agcgccggag gaatatcgcc catcgtctgt
    26401 ttgggtaaca tagccagcac gctactgttc agccgtgatt gcggcagcag gatcagcaac
    26461 gccaccgcca tgaccaggca gactagcccc cataacagcg cggggcgttt actgggcggc
    26521 aaaacgttgg cgttcgtcat cggtcagttg cgctggcgtc agttgatgtt gggtaagagc
    26581 aatatcggtg cgatcgccct gtttatcatt aagttgaatg ctctccagat aggttttccc
    26641 ggcgagatcg atggtgttga aaattttatc cagcggcgtg gtggtcggcg tcaggcgcag
    26701 cgtccagcgg ccttcgcctt tgtcagcaaa ttcgacgcgg aagttttgtt ccagcacttt
    26761 gcgatcggcc tggaacaacg cgcgcagcag gtggttaaac tggaacatct gcgggttgtt
    26821 ttctgccgtg atgatttgcg gcggctgacc gttgatcacc tgcaccatgc gtttatcgtc
    26881 cagtaatagt tgcatgggga acggtgaggt ttgatcccac aacagcccct ggtcgcgggc
    26941 gatcaacatc tggccctgag atcgcagcgg ctgcggcaga tctttaatcg tccgggtttg
    27001 atcgaaatgg gcgcggatca ccggttgttc ggtaaagcgt tgctgaagat cgtccagggt
    27061 cagggcgctc acaaacgggc taatcagcag cgccagcagc ggtaaaaatt tcatggtttg
    27121 actcccatgc gttcaaacag aatatcgggg ctgacaaagc acagttcgcg actttgctcg
    27181 tcgaccgcga cctgaatggt gtatccggtg gtggcgcgtt tgcccgtttc ggcatcgaaa
    27241 atctgatagc caatccgcag acggttttcg aactcttcga tatgcgcacg cacgcggatg
    27301 cgttgctcaa aggtgagggc gtggcgatat ttcacccgcg catcgaccac tggccacaaa
    27361 taaccggact ctttcatttg ccgatagccg taattgaact ggttgagcag cgcctcgcga
    27421 gccacttcaa agtagcgaaa atagttaccg tgccaggcca cgcccatcat atcgacgtcg
    27481 tgaaagggaa tggtcagctc gacttctgcg gtaaatcggg gatcgttcag caccctttac
    27541 tccttgtcct gaatttccgg cagttgccag aaatcgaaaa aattaaacca gtcgagaggc
    27601 gactggagcg cgtaatgttc cagacgcgcg gcgtaatgat cgatagcgtt ttgcagcgcc
    27661 tgttggcgtt cgccgcgcgg cagctgcagt gggtcggcaa agctttcgca gtgaatatgc
    27721 agcttgccgt gctggcgcag ggcgaaaatc agattcaccg ggcagcgcaa aatagaggcg
    27781 agaataaacg gcccctgtgg gaaaggcgca ggctggccca taaacgaact ccagcagacg
    27841 cgccagtcgc cgccgcgttg cgggttgacg gcgatgcggt caccgacaat cgccacccat
    27901 tcgccgttat ccagcttctc tttcagcagg atggcggttt ctgggccgat atctgttacc
    27961 ggcatcaggt taatgcctgc ctgaggagcc atctcctgca ttatctgttt aaagcgttgg
    28021 gcgttttcgc taaacaccag cgcgttaatg gttttgtagc cctgaatttt tgccagcgcc
    28081 cggcacactt ccacatcgcc aagatgcgag gccagcagca atttgccgcg cggatcgcgg
    28141 acgtcaagcg ctgcttctgc gcctggcgca aacagcacat cacgccctgg ttgtagctcg
    28201 ccgcgccagc tggcgatttt gtcgagcatg gcattaccga aacgtagaaa gtgctgatag
    28261 ctgttgagtt ttgccgcctg tggttgatgc tgtcgtacac gggcgagcca gtcttgcgac
    28321 gctttgcgcg cacgagaagc agtgagccag tagacgccca ccaccgggta aagcagcgcg
    28381 ctaaacgccg ttcttcccag cagacgccag accagcagca tcaggcgcat tccccataat
    28441 cctttcactt cactctgccg cgcccagtgg caggaagagc ggcgcatcag cagtgaagga
    28501 atacgcggca acatgccgaa aaacagacgc gtgtgcatga gcgagatgcg gacgttatct
    28561 ttcagggcat caaaatgcga aagcccgtcc agtggatagg tgacgcgggt cggcacgaaa
    28621 tagctggtat ttccctgcca gtagaggcgc accatcactt cggtgtcgaa atccatccgc
    28681 ttgccgatgg tggcgtgttt tgccagttgc agcgttggcg caaccggata aacgcgaaaa
    28741 ccgcacatgc tgtctttcag ttgcagggag agcgtttcga tccataccca gacgtgggtg
    28801 acccagcgcc cgtaaagccg tgagcggggg atggagtcat cgtaaattgg ctggccggag
    28861 atcagtgccg caggttgttg ttcagcgaga gccagcagtt tagggatatc ttcaatcgcg
    28921 tgctgaccgt cagcatccac ctgcaccgca tggctgaacc ctgcgtctgc cgctgcctgt
    28981 aagccacgca tcaccgccgc gcctttgcct gcgttttctg ccaggcgaat taaggtcacg
    29041 ccaggctgtt cggcaagcag actgtccagt tgctgttgtg tggtggcatc gctgccgtca
    29101 tccaccacaa tacagggcag attaaatggc ttaagacgcg ccagcacgcc cggcatcatc
    29161 gcgccgtggt tgtagcaggg tatcaacacg cagggagaaa agtttaccga cataggcgga
    29221 tcttcccgct actggcggtg tggcgggcgt caccgtcgtg acgctgatag ctaaagctca
    29281 gaatctggcg ttcttcctgc cagttaagcg cgagcgtgac gcggttttcc ggcagcaacg
    29341 gagactggaa ttttatgttc tgaatggagt gaaaacgcca gccaggcgcg agcagattga
    29401 gcgcgtagct catcgcccag tcgatttgcg caactcctgg gagtaatggt tgcacggcaa
    29461 aatgaccgcg aaaccagaac agatccgccc tgagatgcag aacaatttca aggtgattcg
    29521 tttgtgcctg atggcgctcg atttcatggg gcttcatgaa ataactcctg taattgcgca
    29581 tagacacgct tgttcatact gtttactggc atttcatcaa taacgcgcca gtaacgcgga
    29641 atagcaactg gctccagcgt cgggcgcagt aaacgccgcc aggtcatttc ctggctatgc
    29701 ccgccgccgt tctgccattg cagatgagct tcgtcatcca gcaccagcaa aacgcctatg
    29761 ctctgacgcc cgccgcgcgt aacgggaact gccgccgcct cgcgaatgcc gtccagcgcc
    29821 agtagacgtt gttctacttc ctggagtgaa atacgttttt cttcaatctt aacgatgcgc
    29881 ccacgacgcc ccatcagatg gaactgacca tcttcgctaa actgtaagat atcgtcgagt
    29941 aacatgccgt tatcttccgc tatcagcggt gaaaagaggc gaaaggcatc gccttcggcc
    30001 tggaactgca tacctgggag gggtagccag cggcgttgct cttgttcgcg gtagcgccag
    30061 gcgatgacgc cagtttccgt gctgccgtag atttcatcag gccagacgcg cagccagctc
    30121 gcggtatgtt gtacatcctg ccacggcagc tcaccgccag cagagagaag cacctgaacg
    30181 ggtggcggag aaagctgggt atccaggcgc ttcaaaaatg ccgggctgct gatgaagata
    30241 tagcgatgct gatgactcaa cgcggcaaac tgttcgacat accagagcat ggcggcgtgc
    30301 agcggtaatc ccaacgccat gggcaggaaa acgcgaaaag tcaggccgta caaatgctgc
    30361 ggcaatacgg aaccgacgac acgacaaccc gccagacgtg cgcctaatcg ttcagccagc
    30421 agttctgctt cacgatccag caaatgaaca ggtttaatcg cccgtttcgg ctgtcccgtg
    30481 gaaccggagg taaacagctc gataaaagcc tctggcgcta tagcggcaaa ggtgaaggat
    30541 tgtgtcgcga tttgtggaga acttgctacc aacagcaatg aaccttgcca gttaagttcg
    30601 ctgtcgctaa gcacaccatc aaaaagttca cgttgctcgt taagctggat aacacggtta
    30661 tgccccggaa gcaccggcgt ttttccggcg tgtagcgtgg cgagcagggc aacgataaac
    30721 agatagctgt tctcaaagca cagcgcccag cgttcacccg gctgttgctg caagcggcag
    30781 ataagttgcg cgacatcgtg acgcaaatcg cccagcgtcc aggtactttc gttcagccac
    30841 gcaatgggtg tgtcatcagg gcgtggcgca gtcagccagc gtgcaagcgg taaggtctgc
    30901 ttcatgcgcg atctcttttc atcacctgac ggcgcagcag ccattcacca gccattaacg
    30961 tccccatcaa caaataagca atcatgccgt tccaggcggt ccataacgac atatcggcat
    31021 aaagcgcggt aaagagggca attccaccgt taatgatgaa aaagccgcac cagatttgcg
    31081 tgacgtggcg ggtatagcgc acgccctttt ccgggaggtc tggctcctgc aaccgcgcca
    31141 gccgctcaac tatcggcatt gccgaccaca gcgaaccgcc aaaaaccgcc agcatgaccg
    31201 cgttaaccac taccgggtaa aacagcagta gctggtgcgt tttcaacata aagctggcga
    31261 cacataatgc gatgcccacg acagcgacga gttgcgtgac ggcttgtaaa ggccccgcct
    31321 ggcgacgggt ctggcgcaga cgcaacaaaa gcagcaacgc cattagcggt aacagccagt
    31381 gcaggccgtt atgcgctaag ccaaaccaaa tcacaaacgg ccaggcgagc agcaataatc
    31441 cagtcagcag cttaatcatc ggtagcgagc gaatgcccga catcagatca ggcttcttgt
    31501 agcaggcgtt ctacagcctc gacgacatcc tggactgtac gcactgcttt aaactcttcc
    31561 ggcttgattt tcttaccggt tttcttttgc aggtgcacaa tcatgtcaac ggcgtcgatg
    31621 ctgtccagtt ccagatcttc gtacaggcgc gtttcaggtt tgatgtcctg cggatcgatt
    31681 tcaaacagct taaccagcag ggctgagact tcctgataga cggtttgttg ttctgtcatg
    31741 atggctttcc tcaggcacgt tgcgcagcaa tgaaagaggc cagagtggcg acggagaaaa
    31801 agtgctgacg catctcttcg ctttccgcag agagcacgat gccgtactca ttttttactg
    31861 ccagtcccag ttccagggca tcgatagagt ccagccctaa accgtcgcca aacagcggcg
    31921 cgtcggtatc gatatcatca ggcgtcaact cgtccagatt gagggtgctg atgatgaggt
    31981 ttttaatttc cagataaagc gcttgcatta tttattcctg agagaggtaa acaaccttgt
    32041 tgtaattgaa gcaaaagatg ccggtttaac tgccttgctg ccagcgccgg ttcttgtgaa
    32101 gttgcatcgt aaaatttatt gatttcaacc cgaccatgga ctgcaaccgt aaaaaacggc
    32161 cggtcaggtg gaacgtcata ccatttactt tgtttatcca gcagatgctc gctacagccg
    32221 attgtcacga tgcgtaaatc gctggcgcag cgcacggcaa tattcgccgc cccgcgctgc
    32281 agcgtcattt tttcaccggg cttcgttcgt gtgccttcgg ggaaaatcag gatagtgtca
    32341 ccctgtgcca gtcgctgttg gcagcggggt aacagagttt cagcttcgct gttgattaaa
    32401 taatctgtcg cccgcacgac gccgccaaga aaaggatttt ttaacagcgc gcttttcacc
    32461 aggcagtcag tttctggcat gactgacgcc agcagcacgt aatcgatcag cgtgggatga
    32521 ttggctacga cgaggcaacc gcgttcctga tgtaagattt cagcgccatc aatacgataa
    32581 tccagtacgc caagcccttt cgcgacagtt aaaaacaggc gaaaactggc ggcaatgctg
    32641 cgacgcgcca aacgacggcg gcgggaagta tcccacacca aaactaacaa tatgttaaac
    32701 cacacgacag aaagcagcag gccgccgaga ccaaacaggg caaaacataa cccggtcatg
    32761 accagtcgcc aggtccactc cagacgtgac atcagcttac tcatctgtgg ctccagcgcc
    32821 attgcacgcg ctcgccaggc agtgaaaatg tgtctacatt ttgtaaatag tgctgtaaaa
    32881 acaacatgct ttgcggcagt gttgtttcat tgcccgcaat ggctgactgc gtttcacatt
    32941 gccagtcatc gccagcttca atcaccagcg cgacggcgta cggccaggtc ggcatttctg
    33001 atggcagttg tgggtgataa aactcgggca ggaaaccgtc aaaatcgacc atcagcacgc
    33061 gctggtatcc cgcttgtagc aggctaagca cttcacacaa accttgctgg aaactatcgc
    33121 gaccagccga aagcgatgac gaaacgctcg gctgtttggc cgcaatggtc aggttaccca
    33181 cggaagagtt gtgtacggag agcgcgaagt ccgtcggaga aagcgcctgt tcggttgcca
    33241 gtgcatgaac aatgcggtaa ttgcgctcca gttcgccatg acgactggta tacaaaacgg
    33301 cgtctggttg atggcggcgt agcattgcca gaccgcattc aacagccagt ttgctcccgg
    33361 agctgaggcg gcgggcggtc atcattggca gttcacttaa ttttgccaac ggtgctgtcg
    33421 ggtcaattgc ctgaggctga cgtgaccact cctgccattg cagcgtttca ctaagtccag
    33481 gcgctcttgc ctgccagtcg agaatgttta atgcaaattt catcaacgcg cgcccgcggt
    33541 aaaccaaaat tattaagtga cgattatttc ttttggcata tcaaaagtgt atgcccaacg
    33601 cctaaattat cgtggcgctc tgctacctgg aatcctgctt tattcagata gtcgtaaaat
    33661 ttctctgcac tatagaaacg gctgttaccg tttgccatgc aggtaaaata aagtgaggac
    33721 gcgttcaggc taaacgatgc tgcttcaaac cgctgcgcat cccagaacaa ctccatgatg
    33781 caaacttttg cgccaggctt cattacgctg gcaattttgc tgagtatgct aattatttgt
    33841 tcgggtgaaa aacagtctaa aaattggctc atccaccaga tatcagcctc gcccggtaat
    33901 ggggcatcgc ttagcatatc caccgcatgg aaatctatgc gatcagaaaa acctgcattc
    33961 gcgatatttt ctttcgctag tacgatttgt tgtggcagat ctaataacgt aacggcgatg
    34021 ttttcattgt atttgcagca acgcaaagcc cacttacccg tattgccgcc tacatcgtac
    34081 aatgttgtgg ggttgttggc gaatacataa ggcagtgcgg cgttaaatgc gccatctgaa
    34141 tagtaatgat caaacgcaaa ccagctttca cgagcggcgt ctggtaattg cgaaagtgcc
    34201 ggatatatcg tcgaccagtc gccaaatact tttaacccgg aaggttttcc ttcattgagt
    34261 gaatcggcga ggaagaagag cccctggtaa cagacatcct gggtgaaatc catattcacg
    34321 cgagtcatag tgtcatgcag gaggtaatga ccgattttcg ccaggtaata aattccctct
    34381 ttacaggtaa taattcgccc gcttaagccc atatctaaca gaacgctggt agcatattcg
    34441 ttgatatggc tatgttcggt gatatcagcc agcgttgcgc catgtttgcc ttgccgatcc
    34501 agatatgaaa gtacacccgc attacgtagg cagagtgctg tttgaaaaag cataggggca
    34561 aaggcaatgc gttgagcttc ggtaatcgca tccagtgcgc taagagaatc ctgttcgtac
    34621 atgttgggca caacctgtta agagggaatt aaattattat cggaccgcaa cggcccgact
    34681 ttaggaagaa ttataaatct gcgcccgctg acaaattcag ttgaatatct ttgtcgagat
    34741 tgcggaccca gcgattgtag tttttatgaa tttgtccgcc tgacgcttgt aggttctggc
    34801 tattttcata attgatatcg tagctggatg ccgtgtagtt aatgcgtatt tccgctgaat
    34861 gatcgcgtga ctgttgacgt gctttaatta tgccttgtcc ggcttcggac ataatccatt
    34921 tgcgttcaac gccagctttc agaatcgctt ttttcacttg ttcctgggta tgaccagcgc
    34981 tgacggttga gtgaacctga tcaattggcg cagtacgcgc gcaacctgct aaagcaccca
    35041 caacagcaat agccgcaaac catttcatgt aatgtctcat tagaacccca ttaaagtatt
    35101 gtttttacga gtaaagcaag ttttaactgt aattgctggc atcactattc tttatttaga
    35161 gaaaaaatta cttgtgagat aagtcagtaa gtgcacatca gagtaaaaat ctctgaaatg
    35221 actaaattac tgccagcagg gaccaactat aagtgagtga gaaaaatgag gcaataaaaa
    35281 taacacaatg ttatcagtgc attctttgag aaggatttaa tcgctattcg gcgtttatat
    35341 atatttgact taaggaatgg tttttttaaa ttaattttct gaataatgtt tcatcatttc
    35401 ctcaaatcaa atggccactg ttagataaac ggtggccatt gcctgctcag gaggtgctat
    35461 taacccggta accgactttt cggtctgccc ggccccagta aaatcgccag tttgctgccg
    35521 cctttcgtcg tttccatcca gattttacac acgctggtta atggcaccga aagtagcatc
    35581 cctaccgggc cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac
    35641 atccccaggc gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc
    35701 actaaaaaca atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga
    35761 atcattggcg gtacggcgga aattaccgcg ccgatattgg gcacgtagtt aagcaaaaac
    35821 gccagtactg cccacatcag cgcaaactgt acccccatca gctccagccc cagccagacg
    35881 atgacacccg tccataaact gagtagcgtc tttaacgcca gataatgcga aacgccttta
    35941 agtgcgcggt gtaatcccgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg
    36001 taagggacgt ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg
    36061 ctcgccattg ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc
    36121 gagtccatcc gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc
    36181 tcctgcaatt taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata
    36241 aattcgttaa acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc
    36301 accactacaa tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtgacg
    36361 agcgggttga ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc
    36421 gctgcgtgaa tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg
    36481 cccgttttat cgggttgagg ggtttccatt ggggcttcct tgtgactttt tgtattaagt
    36541 gtagcgggag tcacgacggc aatattctga ttcttactgc tgattttcac gtcagcacgt
    36601 ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga
    36661 acccgcgcta aacggattgc gcctgaattt gcgcattctc tccattgtca tgtttaactt
    36721 cgccagctac ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt
    36781 gatgggcttt agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt
    36841 gctgagccgt cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt
    36901 cttcggttta tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc
    36961 cagtctgccc gtcatcagcc tgttattact ttgcctggga cgcgtcatcc ttgggattgg
    37021 gcaaagtttt gccggaacgg gatcgaccct gtggggtgtt ggcgtggttg gctcgctgca
    37081 tatcgggcgg gtgatttcgt ggaacggcat tgtcacttac ggggcgatgg cgatgggtgc
    37141 gccgttaggc gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taattattat
    37201 gggcgtggcg ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa
    37261 aggcaaaccg ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcgct
    37321 ggcactggct tccgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgt
    37381 taaaggttgg ggcggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac
    37441 gcgtttgtta ttccctaacg gcattaaccg tatcggcggc ttaaacgtag cgatgatttg
    37501 ctttagcgtt gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc
    37561 gaaaatcggc gtcttactgg cgggggccgg gttttcgctg gtgttcccgg cattgggtgt
    37621 agtggcggta aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cctacaccgt
    37681 atttatggat ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagttgggc
    37741 gggcgtaccg gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac
    37801 gtggcgatta aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa
    37861 cttactgaat aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat
    37921 cgtcagctgg cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa
    37981 cggaagagta cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt
    38041 gacctttcag ctcaatggct ttattggtga ccacttgcag ctgtggatcg cggctacgtt
    38101 gctgatcttc cagacgcttc gccagcaccg ccagatcttc tttcggatca tcgcccatga
    38161 tgacgatgac tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg
    38221 ttcccagctt accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat
    38281 caagcaggtt gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag
    38341 cgttagtgtc tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat
    38401 atttaaccag attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca
    38461 tccatcttat cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg
    38521 atttatctgc cagtcttttc aacagcatat tgagtaatac gccatacatt ggcaggaaga
    38581 aaacgatact gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt
    38641 cagccataaa ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc
    38701 tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca
    38761 ggcggttaaa aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg
    38821 tggcgatacg ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccatgaac
    38881 ccatatagaa tagcgacgag atgacgtagg agatcagcaa tgccgggatc atcaccgcaa
    38941 agatgatacg acgggccagc ggtgcgccaa aaatacgtac ggtcaggtcg gtagcgagaa
    39001 agataaacgg aaagctaaac gcgccccagg tggtatggaa accaaaaatg gagacgggaa
    39061 gctgaaccag atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca
    39121 acgccttata gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat
    39181 tggggtgagg gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta
    39241 cccttttttc gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc
    39301 acgcaatcgt taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt
    39361 tatgtgatga gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac
    39421 ttggcctgcg ctgcccggaa ccagtcatga tggtgcgcaa aacagtgcgc aatatgcagc
    39481 caggtgaaac attgctgatt attgctgacg atccggcgac gacccgcgat atccctgggt
    39541 tttgtacctt tatggaacac gaactggttg ccaaagagac ggatggactg ccttatcgtt
    39601 atttgattcg taaaggcggt tgacgggggc agattggctt cgatgccgcc ttttcccctc
    39661 accctaaccc tctccccaga ggagcgaggg gaccgatcgc gctcaatgtt gcgatcggtt
    39721 tgccttatct cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg
    39781 tatctgccag cactgccagc catagtccgg tcatccctaa cagcgtggtg acgaggaaga
    39841 tccctttcag ccccagcgca atagtgatgt tctggcggat attggcgtga gtggcgcgtg
    39901 ccagttcaat catttgcacc agaccgcgca ggtggttatg ggttaatgct gcgtcggcgg
    39961 tttccagcgc cacgtctgtg ccgctgccca ttgcaatccc gatggcggca gctttcatcg
    40021 ctggcgcgtc gttaataccg tcaccgacca tcgccagcgg cgcatgttga ttcagctcgg
    40081 tcaccgcttt gactttatct tccggcaaca ggcccgcttt aaactccagc cccagctccc
    40141 cggcaattgc cgccgctgcg cgtggattat cgccggtgag gatcacccct ttgacgccca
    40201 gcgcgttcag ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa
    40261 tgatacccag cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca
    40321 gttcattaat caaaccagca aatgcatcag cgggatgttt cccggcagcg catatcaaca
    40381 cgcgctcacc gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg
    40441 cggcgggaat ggcgagtgca gcaacctgtg cttcgcgcac gatggcctgc gccagtggat
    40501 gcgtcgcgcc ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg
    40561 ttgccggatg aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa
    40621 acgccacctg ggtaacacga cccagctgtt ccagcgccgc tccgccttta atcaacgccc
    40681 cacgacgcgc tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg
    40741 cacacgggca gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg
    40801 tggcaaacag cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg
    40861 tgtaaatacg gctgaaacgg tcgataaacc gctcaatggg tgcgcgacgc tcttcggctt
    40921 cttcgatcag tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca
    40981 gcgttaccag acggtctacg ctggtggctc ctgcaggaac tttatcgccc gttgcgcgct
    41041 ccaccggaat agattcgccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca
    41101 gtttaccgtc ggcaggcaaa cgtccacctg cggcgacttc aatcacatcg ccagggcgca
    41161 ggctgttaat cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt
    41221 tcagcgccat taacgcgctg accccttgac gcgcgcggct ggcggcccag ccttccagtc
    41281 gttcaccaat caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca
    41341 gtgcaccaat agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga
    41401 tcagccgtaa tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg
    41461 ccagttgccc gaacggatga ttaaattgct ccaaacccca gctgattgcc atcatcacga
    41521 taagcgtaat cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct
    41581 gctcatcgcg cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacggatgt
    41641 cattgtcggc atcaaccacc agtttttcgg tggcgaacaa cacctgtacc tgattcacgc
    41701 ctgcaagctg gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccgc
    41761 tgactttcca gctatagcgg gtgccggaga cgttttcaga gagagttggc gtgctggaac
    41821 atgcgccgtc gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag
    41881 caaattgagg ggctttcttg ccgtgattgt caggagtcga catggcatcc tccggttaag
    41941 ttttttctca ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag
    42001 agatacagcg agcggacgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg
    42061 ttgtccgcac ggaagcgtcg gcgatagtga ctacccagcc agacaaagtt actgataaac
    42121 agcaacgaag cgccgacaaa cgcagagagt gccggagcgg tcggacggaa gaaccacagt
    42181 tcacctgcca gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga
    42241 tactcttcca gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc
    42301 agtggcagag gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc
    42361 aggtgcgaga ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt
    42421 agcgcatcgc ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg
    42481 aacatcggcg cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc
    42541 cagcgttgcc aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag
    42601 acagcgataa acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga
    42661 atgcctacgg tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact
    42721 ggcagggtat gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct
    42781 tttctttatt gttatcattg gcttaatcat cgtcgccgca tcgtttcgtt ttatgcaaca
    42841 gcgacgggaa aaagctgata atgatatggc accactccag caaaagctgg tgttggtgag
    42901 caacaagcgg gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc
    42961 aggcaccagt atgcgctatg aggcaagctt caaaccgcaa agcggaggaa tggagcagac
    43021 gtttcgcctc gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag
    43081 ctataaagga acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt
    43141 aaatttcttc tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gcaccttttc
    43201 ggcggtggtc atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagttgcat
    43261 accatgcatc agcacggtaa aaatcagcgc cacgttaacg aaaatattca gcgggcgcgg
    43321 gaagggatgc accaggttga ggattaaaaa tccccaaaca cagagcatta acaaacgacc
    43381 aatattaatc agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct
    43441 gacccgccac tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt
    43501 cgacttcgct ttcgacataa atcagggttt cgtcagccag ccagccgtta tcttccagta
    43561 aatttatcgt ctcttctaac aaaccacggc ggaacggtgg atcgacaaac acgatattat
    43621 gcggtgtgcc tttttgcgcc aggaatgaca ttgcgttgct gttcaccacg cgtgcattgc
    43681 ctgcttttag tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa
    43741 ttaacgtggc ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg
    43801 cgaagcaatc cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt
    43861 cgcgtacgcg gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc
    43921 ctcgccactg cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca
    43981 tgataaattg ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt
    44041 aagtgataga ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc
    44101 atgaagcaac agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt
    44161 tcctggctgg gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat
    44221 gaacaaacgg ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttctgaacaa
    44281 gccgtcgaag agcagccgca ggcgcatact gaggccgagg cggaaacttt tgctgccgac
    44341 gttgtggaag tcactgaaca ggttgctgaa aatgaaaaag cgcagcctga agcggaagtc
    44401 gttgcacaac cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa
    44461 gagctgccgt tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct
    44521 gaagcggaaa ccgtagagat tgtcgaagcg gcggaagaag aagcggctaa agaagaaatc
    44581 accgacgaag agctggaagc acaggcgctg gctgccgaag cagcagaaga ggcggtgatg
    44641 gtggttcctc cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa
    44701 ccgaccaaag aaggtttttt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat
    44761 ctcggttccg gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag
    44821 gagctggaag agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc
    44881 accaatctga cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc
    44941 ctgctgaaag aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa
    45001 ggtaaaacgc cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg
    45061 attggtaagc tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc
    45121 gatactttcc gtgcagcagc ggttgaacag cttcaggtct gggggcagcg caacaatatt
    45181 ccggtgattg cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag
    45241 gcggctaaag cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac
    45301 aaatcgcacc tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt
    45361 gaagcgccgc atgaagttat gctgactatc gatgccagca ccgggcagaa cgcggtaagc
    45421 caggccaaac tgttccatga agccgttggc ttaaccggca tcacgctaac gaaactggac
    45481 ggcacggcga aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc
    45541 tacattggtg tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata
    45601 gaggcacttt ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt
    45661 atctcggtgg gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg
    45721 cgtttctgac cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga
    45781 ttgagcggcc cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa
    45841 accgtgaagt tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac
    45901 tgatggaccg tactgtctac gataacgtgg cgatcccgct gattatcgcc ggtgccagcg
    45961 gtgacgatat tcgccgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag
    46021 cgaagaactt ccctattcag ctttctggcg gtgaacaaca gcgtgttggc attgcccgcg
    46081 cggtggtgaa caagcccgcg gtactgctgg cggacgaacc gactggtaac ctggacgacg
    46141 cgttgtcgga agggattcta cgtctgtttg aagagtttaa ccgcgtaggg gtaaccgtat
    46201 tgatggcaac gcacgacatc aacctgattt cacggcgttc ctatcgcatg ctcaccctga
    46261 gcgatggtca cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt
    46321 cggcagtttg gcgggcgtct tgatcgcttc cgtaaatcgg tcggcggctc aggcgacggc
    46381 ggtcgtaacg caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac
    46441 gttttcaacg aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa
    46501 ccgttcgcca catttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc
    46561 tgttatatgg tgtacaaaaa cgttaaccag gcggcgacgc agtattatcc gtcaccgcaa
    46621 atcactgttt atctgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag
    46681 ttgcaggccg agcaaggcgt agagaaagtg aactatcttt ctcgtgaaga cgcactgggc
    46741 gagttccgta actggtctgg ttttggtggc gcgctggata tgctggaaga aaacccgctt
    46801 ccggcagtgg cggtggtgat cccgaagctc gactttcagg ggacggagtc tctgaatacg
    46861 ctgcgtgatc gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg
    46921 tttgcccgtc tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg
    46981 ttgatggtgg cggcagtgtt cctcgtcatc ggtaacagtg tacgcctgag tatctttgct
    47041 cgtcgtgact ccattaacgt gcagaaactg attggtgcaa cggatggttt catcctgcgc
    47101 ccgttcctgt acggtggcgc attgctgggg ttttctggcg cattgttgtc attaatttta
    47161 tcagaaattc tggtactgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga
    47221 acgaagtttg atatcaatgg tttatcgttc gatgaatgcc tgctgttgct gctggtatgc
    47281 tcgatgattg gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg
    47341 cctgaataat aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga
    47401 gaccccgttg tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc
    47461 acaagcctgc attgaacttg tggataaaat cacggtctga taaaacagtg aatgataacc
    47521 tcgttgctct taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat
    47581 tgagaggatt tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct
    47641 ggattcctac atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc
    47701 gctggctgaa aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc
    47761 tcacctgcgg tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc
    47821 ggatttgatt caggaaggta acatcggcct gatgaaagca gtgcgccgtt ttaacccgga
    47881 agtgggtgtg cgcctggtct ccttcgccgt ccactggatc aaagcagaga tccacgaata
    47941 cgttctgcgt aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt
    48001 cttcaacctg cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat
    48061 ggtggcccgt gaactgggcg taaccagcaa agacgtacgt gagatggaat cacgtatggc
    48121 ggcacaggac atgacctttg acctgtcttc cgacgacgat tccgacagcc aaccgatggc
    48181 accggtgctc tatctgcagg ataaatcatc taactttgcc gacggcattg aagatgataa
    48241 ctgggaagag caggcagcaa accgtctgac cgacgcgatg cagggtctgg acgaacgcag
    48301 ccaggacatc atccgtgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact
    48361 ggctgaccgt tacggtgttt ccgctgagcg tgtgcgccag ctggaaaaga acgcgatgaa
    48421 aaaactgcgc gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg
    48481 agtccggggt ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc
    48541 caatccccac gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc
    48601 gggtatgttt tagcagagta tgctgctaaa gcacgggtag tcatgcataa aacgaaataa
    48661 agtgctgaac aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat
    48721 gaacataaag ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc
    48781 tctggcagaa gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta
    48841 cggtgaccag gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg
    48901 cattaaaggc aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc
    48961 ggttgcggtg gcgaacaaag tcgttaacga cggcattaaa tacgtgattg gtcacctctg
    49021 ttcttcatca acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac
    49081 cccagcggca accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac
    49141 cggcctggac tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc
    49201 gcagcgtatt gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt
    49261 gcaggacggc ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg
    49321 ggaaaaagat ttctcaacgc tggtggcgcg tctgaaaaaa gagaatatcg acttcgttta
    49381 ctacggcggt tatcacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggtt
    49441 gaaaactcag tttatggggc cggaaggcgt ggctaacgtc tcgctgtcta acattgcggg
    49501 cgaatcagcg gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa
    49561 caaacccatt gttgatgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg
    49621 gaccacctac gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc
    49681 tgaaatcgcc aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacctg
    49741 ggatgagaaa ggcgatctga aaggctttga gttcggtgtg tttgactggc acgccaacgg
    49801 cacggcaacc gatgcgaagt aacagataga taaatgaaaa tgcccgatgc gctgaactgc
    49861 catcgggttt ttttgtgttt gtcgaaatgg ttgtagaaag attaaacaat ccaggatatg
    49921 cttaatgtac ggtgacatac cgtacatatt atacggagca ggagagttca catgtctgct
    49981 gttaaaaagc agcgtatcga tctgcgttta actgacgatg acaaaagtat gattgaggaa
    50041 gcggcagcga tatctaatca gtccgtcagc caatttatgt tgaacagcgc ttcgcagcgg
    50101 gctgcggaag tgattgaaca gcatcggcgg gtgatcctca atgaggaatc ctggacgcgg
    50161 gtgatggatg cgctgagtaa tccaccgtca ccaggtgaaa agctaaaacg tgcggcaaaa
    50221 cgtcttcagg gaatgtaata atttatggat gatctgacga tagagattct gaccgatgat
    50281 gcagattatg atctacagcg attcgactgc ggcgaggaag cgttaaatct ctttctgacg
    50341 acacatctcg ttcgtcaaca tcgcaacaaa attctgcgag cgtatatcct ttgtcgcaac
    50401 actccagaac gtcaggtgct gggatattac acattatgcg gcagttgttt tgaacgagcc
    50461 gcattgccct cgaaatcgaa acagaaaaaa attccctaca aaaatattcc cagcgttact
    50521 cttgggcgtc tggcaattga tcgttcatta caggggcagg gatggggagc aacactggtt
    50581 gctcatgcca tgaaagtcgt ctggtcagcc tctttagcgg taggtattca cggtcttttt
    50641 gtcgaggcgc tgaatgaaaa agcccatacg ttttataaat cgctgggctt tatcccttta
    50701 gtcggggaaa acgaaaatgc gttatttttc ccaaccaaat ccattgaact gctttttaca
    50761 cagagcgatt aacgcttctc ccagccgccc tgctgtgccg taaatcccag tgcctgcata
    50821 aacgccgtca tcacaccgcg atcttccacg cctgcatccg ccatccacca gcatgaaacg
    50881 ccaggattgt tacgcaaaac ctcttccagc agatattgcc ccacaccgcg acgtcgagtg
    50941 acttcccgca cgcgcaggga atccagtgcc ccctcggtgc cgcttaaggt tacccgcacg
    51001 gcagcaagca ggcgctcgtt aaaacgcgcg gcgtagatgc ggtggttatc gtcaacctgt
    51061 aaagaggaag gggaatactc cggccagatc ttttgcaggt caatccggtc ctggtcgcta
    51121 aatttttcta atcgaatgat ggtcagcttc atgggtaacc cgtgtaaatc acaaaagtgg
    51181 aaccagtgta gcgaaataat ttaatcggag gctttctctt ttttatttct tttggcaggt
    51241 gattaatttc ttaacagcaa taattacaaa gttaaaacat tagagaatga aaaataccca
    51301 gcataatccc ctgaatggta gtgaattatt ccgccctttg tgccgttatt ttatgctgac
    51361 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
    51421 ctgaaaaata gggactttaa tcgctattat cacaaaatac tgcgctaaac ccttaatcag
    51481 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
    51541 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
    51601 tcgcagggat gattgcactg gcaatttcac acaccgcttt agccgacgat attaaagttg
    51661 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
    51721 cgcgtcaggc gattaaagac attaatgcca aagggggaat taaaggcgat aagctggttg
    51781 gcgtagaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
    51841 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc cagcctgcgt
    51901 cagatatcta tgaagacgaa ggtattctta tgatctcgcc gggagcgaca aacccggagc
    51961 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
    52021 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
    52081 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
    52141 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
    52201 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
    52261 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aacccagttt atggggccgg
    52321 aaggtgtggg taatgcgtcg ttgtcgaaca ttgccggtga tgccgccgaa ggcatgttgg
    52381 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
    52441 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
    52501 ctctggcaac tgcccttgaa cgtaccggca gcgatgagcc gctggcgctg gtgaaagatt
    52561 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
    52621 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
    52681 agtgaaaatc ccaccgcccg tgaaatgcgg gcgggtttag aaaggttacc ttatgtctga
    52741 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacttacgc
    52801 gctgattgcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
    52861 cggcgaggtt tacatgatcg gcagctacgt ctcatttatg atcatcgccg cactgatgat
    52921 gatgggcatt gataccggct ggctgctggt agcggcggga ttcgtcggcg caatcgtcat
    52981 tgccagcgcc tacggctgga gtatcgaacg ggtggcttac cgcccggtgc gtaactctaa
    53041 gcgcctgatt gcactcatct cggcaatcgg tatgtccatc ttcctgcaaa actacgtcag
    53101 cctgactgaa ggttcgcgcg acgtggcgct gcccagcttg tttaatggtc agtgggtagt
    53161 gggtcatagc gaaaacttct ctgcctctat taccaccatg caggcggtga tctggattgt
    53221 caccttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
    53281 ctgccgtgcc tgcgccgaag atctgaaaat ggcgagcctg cttggcatta acaccgaccg
    53341 ggtgattgct ttgacctttg tgattggcgc ggcgatggcg gcggtggcgg gcgtactgct
    53401 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
    53461 taccgctgcg gtgctcggtg ggattggcag cattccggga gcgatgattg gcgggctgat
    53521 tctggggatt gcggaggcgc tctcttctgc ctacctgagt acggaatata aagatgtggt
    53581 ctcattcgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
    53641 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgttgctctc tgccgcgatg
    53701 ttttttgtcc tggcgggcgt ctttatgggc gtacaactgg agctggatgg caccaaactg
    53761 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
    53821 tttttcttcc agcttttgcg accggctttc cagaaagggt tgaaaagcgt ttccggaccg
    53881 aagtttattc tgcccgccat tgatggctcc acggtgaagc aaaagctgtt tctcgtggcg
    53941 ctgctggtgc ttgcggtggc gtggccgttt atggtctcac gcgggacggt ggatatcgcc
    54001 accctgacca tgatctacat tatcctcgga ctcgggctga acgtggtggt agggctttct
    54061 ggtctgctgg tgctggggta cggcggcttt tacgccatcg gcgcttatac ctttgcgctg
    54121 ctcaatcact attacggctt gggcttctgg acctgtctgc cgatagccgg gttgatggcg
    54181 gcggcggcgg gcttcctgct cggcttcccg gtgcttcgct tgcgcggtga ctatctggcg
    54241 atcgttaccc tcggtttcgg cgaaattgtg cgcatattgc tactcaataa caccgaaatt
    54301 accggcggtc cgaacggtat cagtcagatc ccgaaaccga cttttttcgg ccttgagttc
    54361 agccgtaccg ctcgtgaagg cggctgggac acgttcagta atttctttgg cctgaaatac
    54421 gatccctccg atcgtgtcat tttcctctac ctggtggcgt tgctgctggt ggtgctaagc
    54481 ctgtttgtca ttaaccgcct gctgcggatg ccgctgggtc gcgcctggga agcgttgcgt
    54541 gaagatgaaa tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgactgcc
    54601 tttaccatca gcgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgtcagggc
    54661 tttgtcagcc cggaatcctt cacctttgcc gaatcggcgt ttgtgctggc gatagtggtg
    54721 ctcggcggta tgggctcgca atttgcggta attctggcgg caattttgct ggtggtgtcg
    54781 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
    54841 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
    54901 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gttaacggcc
    54961 tgatgatgcg cttcggcggc ctgctggctg tgaataacgt caatcttgaa ttgtacccgc
    55021 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
    55081 tgaccggatt ctacaaaccc accggcggca ctattttact gcgcgatcag catctggagg
    55141 gtttgccggg gcagcaaatc gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
    55201 tgttccgcga aatgacggta attgaaaacc tgctggtggc gcagcatcag caactgaaaa
    55261 ccgggctgtt ctctggcctg ttgaaaacgc cttctttccg tcgcgcccaa agtgaagcgc
    55321 tcgaccgcgc cgccacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
    55381 cgagtaacct ggcctacggt gaccagcgcc gtctggagat tgcccgctgc atggtgacgc
    55441 agccggagat tttaatgatc gacgaacctg cggcaggtct taacccgaaa gaaacgaaag
    55501 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
    55561 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
    55621 cgccgctggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
    55681 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
    55741 tacggcaaaa tccaggcgct gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
    55801 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
    55861 ccgcgcgcca ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
    55921 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
    55981 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgtga tcagtttcag
    56041 gagcgcataa agtgggtgta tgagctgttt ccacgtcttc atgagcgtcg tgttcagcgg
    56101 gcaggcacca tgtccggcgg tgaacagcag atgctggcga ttggtcgcgc gctgatgagc
    56161 aatccgcgtt tgttgttgct tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
    56221 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
    56281 cagaacgcca accaggcgct gaagctcgct gatcgcggct acgtgctgga aaacggccat
    56341 gttgtgctct ccgatactgg tgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
    56401 ttaggcgggt aaattatcga taacccggca gctaagttct gccgggttat tcgtccgtat
    56461 acagacttgt ttcaactgac cgggaggatt aaaaccagac cacttatttg ccacggaaag
    56521 gttccctccc atgcattatg ccgttgttgt catgcttgaa ctgctttgct gtattcacgc
    56581 ttaccgtacc ggacaagagc ggtactggat ttttatcatt ttctgttttc cggtgattgg
    56641 ctgcgtggcg tatttcgtca tagtgatgct gcctgaaacg ggcgctgacc gtcacgggca
    56701 tactttgctg atgcgcctgc aggacaaact caaccctgag cgccacctgc gcaaattaac
    56761 cgaagaactg gcgattgccg agacgaatca aaaccattat gcgttggcta atgaactggc
    56821 gcgtctgggg cgttatcacg aagctgttcc ccattatcag caggcgttaa gcgggatatt
    56881 cgcccatgaa gcggcaatga tgctaagcct ggcgcaggca cagtttgcta ttcaggagtt
    56941 cgccgcctgt cagcagacgc tggaagatgt gatgcgttat aaccctgatt ttcagtcggc
    57001 agacgggcat ttgttatttg ccagaacgtt agccgcacaa gaaaaatacg ctgatgcgga
    57061 aagcgagttt gaagtattga tttcttatta ccccggcccg caggcgcgta tttactatgc
    57121 ggaaatgttg gcgaaaatga gtcggttacg tgaagccaac gagcagtatg ttgccgtggt
    57181 tgatacggct aaacgctcaa gacctcatta tagaaaacac caccgggaat ggattaagac
    57241 cgccaatgag cggctgaagc agagtgtggt gcaatgaaaa tcttctttgc cgttagacca
    57301 gtgtcgcggc aatcttttgt taaaattgaa aaatgtagct ctcatcgggc agcaaaattg
    57361 gacgcagaat ttgctgccat ttttgctctt catggtaagg agttctggcg attagctggc
    57421 cgttaatcgc ctcttttcag tgcattctcg ggatatccgt gccccatctc ccattttccc
    57481 tcacccgtac cgtcaccgcc ttgtcatctt tctgacacct tactatctta caaatgtaac
    57541 aaaaaagtta tttttctgta attcgagcat gtcatgttac cccgcgagca taaaacgcgt
    57601 gaattcgcgc attcggtaca acaagagaga taaacgatga aaccgttaca ttatacagct
    57661 tcagcactgg cgctcggact ggcgttaatg gggaatgcac aggcagtgac gaccattccg
    57721 ttctggcatt ctatggaagg ggaactgggt aaagaggtgg attctctggc ccaacgtttt
    57781 aacgccgaaa acccggatta caaaattgta ccgacctata aaggcaatta cgaacagaat
    57841 ttaagcgcgg ggattgccgc atttcgtacc ggcaatgctc cggctatttt gcaggtttat
    57901 gaagttggca ccgccaccat gatggcgtcg aaagccatta aaccggttta tgacgtgttt
    57961 aaagaggcgg ggattcaatt cgatgagtcg cagtttgtgc cgacggtttc cggttactac
    58021 tccgacagca aaacgggcca cttactctcc cagccattca acagctcgac tcccgttctc
    58081 tattacaaca aagacgcctt caagaaagcg gggttagacc cggaacaacc gccgaaaacc
    58141 tggcaggatc tggcggacta tgccgcaaaa ctaaaagcct ccggcatgaa gtgcggctac
    58201 gccagcggct ggcagggctg gatccaactg gaaaacttta gcgcctggaa cggtctgccg
    58261 tttgccagca aaaacaacgg ctttgacggc acggatgcgg tgctggagtt caacaagccg
    58321 gagcaggtga aacacatcgc catgctcgaa gagatgaaca agaagggcga tttcagctac
    58381 gtcgggcgta aggatgaatc caccgagaag ttctataacg gtgattgcgc tatgaccacc
    58441 gcctcttccg gttctctcgc taatattcgc gagtacgcca aattcaacta tggcgtaggc
    58501 atgatgcctt acgacgccga tgcgaaagat gcaccgcaaa acgccattat cggcggggcc
    58561 agcctgtggg tgatgcaggg caaagataaa gaaacgtata ccggcgtggc gaagttcctc
    58621 gacttcctcg cgaagccaga aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca
    58681 atcaccaaag cagcgtatga cctgacccgt gagcagggct tttatgagaa aaacccaggg
    58741 gcggataccg cgacgcgtca gatgctgaat aagccgccgt tgccgttcac caaagggctg
    58801 cgtctgggca acatgccgca gatccgcgtg attgtggatg aagagctgga gagcgtgtgg
    58861 accggtaaga agacaccaca gcaggcactg gataccgccg ttgagcgtgg aaatcagttg
    58921 ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt aatgtcggat gcgtttcgct
    58981 tatctgacct ggcatcgcgt gtaggccgga taagcgaagc gcatccggca cagttcagga
    59041 attaaccgta atgtcatcat cccgtccggt gttccgctcg cgctggctgc cttatctgct
    59101 ggtcgcgccg cagctcatca tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt
    59161 gtggtactcg ctgcaaagcg tcgatccgtt tggtttctcc agccggtttg tcggcctgga
    59221 taacttcgtc gcgctgtttc atgacagcta ctatatcgac tccttctgga cgacgataaa
    59281 attcagcacc tttgtcaccg tcagcggttt gctggtatcg ctgttctttg cggcgctggt
    59341 ggagtacatc gtgcgcggca gtcgtttcta tcaaaccttg atgctgctgc cgtatgccgt
    59401 ggctcccgcc gttgccgccg tattgtggat cttcctgttt aaccccggtc gcgggctgat
    59461 cacccatttt ctcgctgagt tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc
    59521 aatgtttctg gtggtgtttg cctcagtatg gaagcaaatc agctacaact tcctgttctt
    59581 ctatgccgcg ctgcaatcca ttccccgttc gttgatcgaa gccgcagcca tcgacggtgc
    59641 agggccgatt cgccgcttct ttaagattgc gctgccgctt atcgccccgg tgagtttctt
    59701 cctgctggta gtgaacctgg tgtatgcctt cttcgacacc ttcccggtga tcgacgccgc
    59761 cacgtccggc gggccggttc aggccaccac tacgctgatt tataagatct accgcgaagg
    59821 ctttaccgga ctggatctgg cttcgtctgc cgcacagtcg gtggtgttga tgttcctcgt
    59881 catcgtgctg acggtgatgc agttccgcta tgttgaaagc aaggtgcgtt accaatgatt
    59941 gagaaccgtc cgtggctgac gatattcagc cataccatgc tgatcctcgg gatcgcggtg
    60001 atcctattcc cgctgtacgt ggcgtttgtc gcggcgacgc tggataaaca ggccgtctac
    60061 gccgcgccga tgacgctcat ccccggcacg catctgctgg aaaacatcca caacatctgg
    60121 gtaaacgggg taggcacaaa tagtgcgccg ttctggcgga tgttgcttaa cagcttcgtg
    60181 atggcgttca gcattacgct cggcaaaatt accgtctcga tgctctcggc attcgccatt
    60241 gtctggtttc gttttccgct gcgtaacctc tttttctgga tgatttttat caccctgatg
    60301 ttgccggttg aagtacgtat cttcccgacg gtggaagtca tcgccaacct gaagatgctc
    60361 gacagctacg caggtttaac gctgccgctg atggcctcgg cgaccgcgac gtttcttttc
    60421 cgccagttct ttatgaccct accggatgaa ctggtggaag ccgcgcggat cgacggcgcg
    60481 tcgccaatgc gcttcttttg cgacatcgtt tttccgttgt ccaaaactaa tctggcggcg
    60541 ttgtttgtga tcacctttat ttacggctgg aaccagtatt tgtggccgtt gttgattatt
    60601 accgacgtgg atctcggcac caccgtggca gggatcaaag ggatgatcgc cactggcgaa
    60661 ggcaccacag aatggaactc agtgatggcg gcgatgttgt taaccctgat cccaccagta
    60721 gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa
    60781 gatggcagga ctgaaattac aggcagtaac caaaagctgg gatggcaaaa cccaggtgat
    60841 taaaccgctg acccttgatg tggcggatgg cgaatttatc gtgatggtcg ggccgtcagg
    60901 ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg gagcgggtga cggaaggcga
    60961 tatctgtatc aacgaccagc gggtgaccga aatggagccg aaagatcgcg ggattgcgat
    61021 ggtgttccag aactacgcgc tttatccgca tatgagtgtt gaagaaaaca tggcgtgggg
    61081 gctgaaaatt cgcggcatgg gcaagcagca aattgccgag cgcgttaaag aggcggcgcg
    61141 cattctggaa ctggacggtc tgcttaagcg ccgcccgcgc gagctttccg gcggtcagcg
    61201 tcagcgtgtg gcgatgggcc gggcgattgt gcgcgatccg gcggtgttcc tgtttgatga
    61261 gccactctct aacctcgatg ccaagctgcg cgtacagatg cgtcttgaac tgcaacagct
    61321 gcaccgtcgc ctgaaaacga cttcactcta cgttactcac gatcaggttg aggcgatgac
    61381 cctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc gaacagattg gcacaccagt
    61441 tgaagtctac gaaaagcccg ccagcctgtt tgtggcgagt tttattggca gcccggcgat
    61501 gaatctgctg acaggccgcg tgaataacga aggcacgcac ttcgagctgg acggcggcat
    61561 tgcgctgccg ctaaacggtg gctaccgtca gtatgcaggg cgtaaaatga ctctcggcat
    61621 tcgcccggaa catatcgcgc taagctcgca ggcagaaggc ggcgtgccgc tggtgatgga
    61681 cacgctggag atcctcggcg cagataacct ggcgcacgga cgctggggcg aacagaagct
    61741 ggtggtacgg ctggcgcatc aggagcgccc gacggcaggc agcacgctgt ggctgcatct
    61801 gccggaaaat cagctacatc tttttgatgg tgaaacagga caacgagtat gagtaactgg
    61861 ccttatcccc gcatcgtcgc ccaccgtggc ggcggtaaac tggccccgga aaacaccctg
    61921 gcggcaatcg acgtcggggc aaaatacggt cataagatga tcgaatttga cgcgaagttg
    61981 tcgaaagatg gcgaaatctt cctgctccat gacgacaatc tcgaacgtac cagcaacggc
    62041 tggggcgtcg cgggtgaact gaactggcag gatttactac gtgtggatgc aggcagttgg
    62101 tacggcaaag cgtttaaagg tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt
    62161 cgcgaacatg ggatgatggc gaatatcgaa atcaaaccca ccactggcac tgggccatta
    62221 acgggcaaaa tggtggcgct ggcggcacgc gagctgtggg ccggtatgac gccgccgcta
    62281 ctgtcatcgt ttgagattga tgctttagaa gccgcacaac aggcagtacc agaactgccg
    62341 cgcggtttgt tgctggatga gtggcgcgac gactggcgag aactgaccgc gcggctgggc
    62401 tgcgtctcta ttcatctcaa tcataagttg ctcgataaag cgcgagtgat gcagttgaaa
    62461 gacgccggat tacggattct ggtttatacc gtcaataaac cccagcgcgc ggcagagttg
    62521 ctgcgctggg gcgtggattg catctgtacc gatgcgattg atgtgattgg tccgaacttt
    62581 acggcccaat agttttcaac ggtatatcag gctgcggcgt gccgggctga ttcagcatgt
    62641 cgccgtttct cctctccggc aacatatgct gctgattcaa cgaactatcc ggattacggt
    62701 tgctgtttaa catcccgcca ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct
    62761 cccccggctg tgactgcaac acccgctgag aattattgtt tatctggttt tctaaatgct
    62821 gttgttgcaa ctgagtttgc gttttcagtt gctgattcag catccctttt tgctggattt
    62881 gctgcgtttg catctgggtt tgcatccgct gctgactggg gatctgatac cccggctggt
    62941 tagggttgtt cagagtatta atgggctgtg caaagccgac aaacggcagg agtgccgtaa
    63001 gaatcagaag tcgtttcatc gcgtatcctc ctctgaagat attctttaag tttactcctt
    63061 tcctgcaatc atcaggatta attcagagtt atataccagg cttagctggg gttgcccctt
    63121 aatctctgga gaataacgat gataaaaccg acgtttttac gccgggtggc cattgctgct
    63181 ctgttcacag gaagttgttt tagtacggta gccgcgccac ctgcgccgtc tcctgtttct
    63241 tacggcgtgg aggaagatgt ctttcatccg gtacgcgcga aacaggggat ggtggcatct
    63301 gtagatgcta ctgccactca ggtgggggtg gatatcctca aggagggcgg gaatgccgtt
    63361 gatgccgccg tggctgtggg ctacgcgctg gcggtaacgc atccgcaggc agggaatctg
    63421 ggcggtggtg gttttatgct gatccgctcg aaaaatggca ataccaccgc tatcgatttc
    63481 cgcgaaatgg cacccgccaa agcgacccgc gatatgttcc ttgatgacca gggcaacccg
    63541 gacagcaaaa aatcactcac ttcgcatctg gcttccggca cgcccggtac ggtcgctggt
    63601 ttctcgctgg cgttggataa atacggcaca atgccgttga acaaagtggt gcagccagcg
    63661 tttaaactgg cacgcgatgg ttttatcgtt aacgacgagc tggctgacga tctcaaaacc
    63721 tacgggagcg aagtgctgcc gaatcacgaa aacagcaagg ctatcttctg gaaagagggc
    63781 gagccgctga aaaagggcga caagctggtg caggcgaacc tggcaaagag cctggagatg
    63841 attgccgaaa acggcccgga cgaattctac aaaggcacga tagcggaaca gattgcccag
    63901 gagatgcaga aaaacggtgg cctgatcact aaagaagatt tagcggcgta taaagcggtc
    63961 gaacgcacac cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca
    64021 tcctccggcg ggatccatat cgtacaaatc ctcaatattc tggaaaactt cgatatgaag
    64081 aaatacggtt ttggcagcgc cgacgcgatg cagatcatgg cagaagcgga gaaatatgcc
    64141 tacgctgacc gctcggaata tcttggcgac ccggattttg tgaaagtccc gtggcaggcg
    64201 ctgaccaata aagcctatgc caaatctatt gccgagcaaa tcgatatcaa taaagcgaaa
    64261 ccgtccagcg agattcgccc cggcaagctt gcgccttatg agagtaatca aactacccat
    64321 tattcggtgg tggataaaga cggtaacgcg gtggcggtga cctatacgct gaacaccacc
    64381 ttcggtacgg gtattgtcgc gggcgagagc ggtattttgc tcaataacca aatggatgat
    64441 ttctctgcca aaccgggcgt accgaacgtt tacgggctgg tgggcggtga tgccaacgcc
    64501 gtcgggccga acaaacgccc gctgtcatcg atgtcaccaa ccattgtggt gaaagacggt
    64561 aaaacctggc tggtcaccgg tagccctggc gggagcctga tcatcactac agtgcttcaa
    64621 atggtggtga atagcatcga ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt
    64681 ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg
    64741 ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca
    64801 caaagcatta tggttgggcc ggacggtgag ttgtacggcg catccgaccc gcgctcggtg
    64861 gatgatttaa cggcggggta ctaaggttag cggccctctt cgtgggaaga gggctgtttt
    64921 cttgaacaag ataaattagt gtctggattg cttt
//
LOCUS       DAZFTN010000015        59836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFTN010000015 DAZFTN010000000
VERSION     DAZFTN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..1071
                     /locus_tag="ACHKV2_001138"
     CDS             55..1071
                     /locus_tag="ACHKV2_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6939062.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1156..1578
                     /locus_tag="ACHKV2_001139"
     CDS             1156..1578
                     /locus_tag="ACHKV2_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6939063.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1575..1778
                     /locus_tag="ACHKV2_001140"
     CDS             1575..1778
                     /locus_tag="ACHKV2_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939064.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1782..2189)
                     /gene="cueR"
                     /locus_tag="ACHKV2_001141"
     CDS             complement(1782..2189)
                     /gene="cueR"
                     /locus_tag="ACHKV2_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO6939065.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2186..3361)
                     /locus_tag="ACHKV2_001142"
     CDS             complement(2186..3361)
                     /locus_tag="ACHKV2_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO6939066.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3358..5520)
                     /locus_tag="ACHKV2_001143"
     CDS             complement(3358..5520)
                     /locus_tag="ACHKV2_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO6939067.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5524..21261)
                     /locus_tag="ACHKV2_001144"
     CDS             complement(5524..21261)
                     /locus_tag="ACHKV2_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO6939068.1"
                     /translation="MDINTIATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNY
                     TATTDAAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTI
                     NPVATDDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPA
                     ADIQAIGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQ
                     ALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVS
                     GTNTAGTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQT
                     VTVTFGGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYS
                     VDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQAD
                     GSWSVSLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDD
                     IINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALG
                     SGPQTITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGT
                     SNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNS
                     NSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGN
                     TYTATVQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGL
                     RVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGV
                     PAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEK
                     GETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQ
                     ASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQ
                     TLTVTLNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHAL
                     VVDITAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLD
                     ASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSG
                     DDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQ
                     ALGNGELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVI
                     TGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNT
                     AGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVI
                     FGGKSYTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDAT
                     APSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWS
                     VSVPTGDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINA
                     TEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDV
                     TITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQP
                     AGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSAS
                     HNVQVNTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTA
                     TVQANLSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDT
                     VAGDDVVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVD
                     VSAWPAGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAAL
                     TLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVS
                     NVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTV
                     TLNGTNYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDT
                     SVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGN
                     WSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNIL
                     NADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQA
                     NYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVT
                     RAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTG
                     SGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVA
                     YSATVQADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAIS
                     INTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVP
                     AADLATLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAG
                     SPLTISGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSAT
                     VNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDV
                     VSVVLNGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVT
                     VSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDAS
                     GNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDD
                     IINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALG
                     NGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGS
                     SSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGT
                     QTSITHPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGG
                     KTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPT
                     VTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTL
                     PASDLTALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEH
                     IQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTIS
                     ATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATG
                     TVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASR
                     SVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATV
                     AANGTWALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVA
                     GDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAG
                     LSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVIS
                     GTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAI
                     GNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNE
                     TAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVI
                     DTTAPDPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGG
                     VTWTTLTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAK
                     SITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAA
                     DSLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTD
                     TGSSATDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRT
                     LTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQ
                     ATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYS
                     ARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVE
                     VVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNA
                     NISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWS
                     KTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQV
                     TVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSA
                     TVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNT
                     TNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPV
                     AVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQS
                     GTTSGVAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYG
                     NTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASN
                     IDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSG
                     GWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSN
                     FNDILTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVG
                     TWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTI
                     VQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(21298..25683)
                     /locus_tag="ACHKV2_001145"
     CDS             complement(21298..25683)
                     /locus_tag="ACHKV2_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO6939069.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25786..27141)
                     /locus_tag="ACHKV2_001146"
     CDS             complement(25786..27141)
                     /locus_tag="ACHKV2_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO6939070.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            complement(27479..28771)
                     /gene="ybaT"
                     /locus_tag="ACHKV2_001147"
     CDS             complement(27479..28771)
                     /gene="ybaT"
                     /locus_tag="ACHKV2_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6939071.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYASNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKRMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(28774..29706)
                     /gene="glsA"
                     /locus_tag="ACHKV2_001148"
     CDS             complement(28774..29706)
                     /gene="glsA"
                     /locus_tag="ACHKV2_001148"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO6939072.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            29968..32472
                     /gene="copA"
                     /locus_tag="ACHKV2_001149"
     CDS             29968..32472
                     /gene="copA"
                     /locus_tag="ACHKV2_001149"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO6939073.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(32687..33028)
                     /gene="ybaQ"
                     /locus_tag="ACHKV2_001150"
     CDS             complement(32687..33028)
                     /gene="ybaQ"
                     /locus_tag="ACHKV2_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6939074.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            33166..33960
                     /gene="ybaP"
                     /locus_tag="ACHKV2_001151"
     CDS             33166..33960
                     /gene="ybaP"
                     /locus_tag="ACHKV2_001151"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO6939075.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            34164..34643
                     /gene="ybaK"
                     /locus_tag="ACHKV2_001152"
     CDS             34164..34643
                     /gene="ybaK"
                     /locus_tag="ACHKV2_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO6939076.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(34680..36332)
                     /gene="ushA"
                     /locus_tag="ACHKV2_001153"
     CDS             complement(34680..36332)
                     /gene="ushA"
                     /locus_tag="ACHKV2_001153"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6939077.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            36550..37770
                     /gene="fsr"
                     /locus_tag="ACHKV2_001154"
     CDS             36550..37770
                     /gene="fsr"
                     /locus_tag="ACHKV2_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO6939078.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            38007..39683
                     /gene="ybaL"
                     /locus_tag="ACHKV2_001155"
     CDS             38007..39683
                     /gene="ybaL"
                     /locus_tag="ACHKV2_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO6939079.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39816..41120)
                     /gene="gsk"
                     /locus_tag="ACHKV2_001156"
     CDS             complement(39816..41120)
                     /gene="gsk"
                     /locus_tag="ACHKV2_001156"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO6939080.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            41272..42231
                     /gene="aes"
                     /locus_tag="ACHKV2_001157"
     CDS             41272..42231
                     /gene="aes"
                     /locus_tag="ACHKV2_001157"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO6939081.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(42228..43190)
                     /gene="hemH"
                     /locus_tag="ACHKV2_001158"
     CDS             complement(42228..43190)
                     /gene="hemH"
                     /locus_tag="ACHKV2_001158"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO6939082.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(43322..43966)
                     /gene="adk"
                     /locus_tag="ACHKV2_001159"
     CDS             complement(43322..43966)
                     /gene="adk"
                     /locus_tag="ACHKV2_001159"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO6939083.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(44147..46021)
                     /gene="htpG"
                     /locus_tag="ACHKV2_001160"
     CDS             complement(44147..46021)
                     /gene="htpG"
                     /locus_tag="ACHKV2_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO6939084.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(46131..46736)
                     /gene="recR"
                     /locus_tag="ACHKV2_001161"
     CDS             complement(46131..46736)
                     /gene="recR"
                     /locus_tag="ACHKV2_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO6939085.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(46736..47065)
                     /gene="ybaB"
                     /locus_tag="ACHKV2_001162"
     CDS             complement(46736..47065)
                     /gene="ybaB"
                     /locus_tag="ACHKV2_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO6939086.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(47118..49049)
                     /gene="dnaX"
                     /locus_tag="ACHKV2_001163"
     CDS             complement(47118..49049)
                     /gene="dnaX"
                     /locus_tag="ACHKV2_001163"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO6939087.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    complement(47721..47785)
                     /gene="dnaX"
                     /locus_tag="ACHKV2_001163"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(49178..49729)
                     /gene="apt"
                     /locus_tag="ACHKV2_001164"
     CDS             complement(49178..49729)
                     /gene="apt"
                     /locus_tag="ACHKV2_001164"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO6939088.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(49882..50259)
                     /gene="ybaN"
                     /locus_tag="ACHKV2_001165"
     CDS             complement(49882..50259)
                     /gene="ybaN"
                     /locus_tag="ACHKV2_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO6939089.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            50329..50856
                     /gene="priC"
                     /locus_tag="ACHKV2_001166"
     CDS             50329..50856
                     /gene="priC"
                     /locus_tag="ACHKV2_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO6939090.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            50870..51034
                     /gene="rsmS"
                     /locus_tag="ACHKV2_001167"
     CDS             50870..51034
                     /gene="rsmS"
                     /locus_tag="ACHKV2_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO6939091.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(51073..54435)
                     /gene="mscK"
                     /locus_tag="ACHKV2_001168"
     CDS             complement(51073..54435)
                     /gene="mscK"
                     /locus_tag="ACHKV2_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO6939092.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(54563..55210)
                     /gene="acrR"
                     /locus_tag="ACHKV2_001169"
     CDS             complement(54563..55210)
                     /gene="acrR"
                     /locus_tag="ACHKV2_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO6939093.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPSDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            55352..56545
                     /gene="acrA"
                     /locus_tag="ACHKV2_001170"
     CDS             55352..56545
                     /gene="acrA"
                     /locus_tag="ACHKV2_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO6939094.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            56568..59717
                     /gene="acrB"
                     /locus_tag="ACHKV2_001171"
     CDS             56568..59717
                     /gene="acrB"
                     /locus_tag="ACHKV2_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO6939095.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
BASE COUNT        12611 a        16576 c        16492 g        14157 t
ORIGIN      
        1 tatttgtttc ttatagaaac attagtacga cttattttga acaagagaaa atgaatgaaa
       61 actgtaaacg tagctttact ggcactcata atttcagcaa catccagccc tgttgtttta
      121 gctggtgata ccattgaagc ggcggcaaca gagctttcag ccattaactc tggcatgtcg
      181 caatcggaga ttgagcagaa gattacccgc tttttagaac gcacagacaa cagccccgct
      241 gcgtatacct atttgactga acatcactac atcccttctg aaacacctga taccactcag
      301 actcccactg tccagacaga tcctgacgca ggacaaaaaa ccgttgccgc tacaggtgat
      361 gtacagacaa ctgcccgtta tcagagcatg atcaacgccc gacagtctgc ggtaactgac
      421 gcccagcaaa cgcaaattac agagcaacag gcgcagatcg tagccacaca aaaaacgctc
      481 gccgcgactg gagatacgca aaataccgcg cattatcagg aaatgattaa tgccagactg
      541 gcggctcaaa atgaggctaa tcagcgcacc gccactgaac aagggcagaa aatgaatgcg
      601 ctgacaaccg atgtggcagt acaacagcaa aatgaaagga ctcaatacga taaacaaatg
      661 caaagtctgg cgcaggagtc tgcccaggca catgaacaaa ttgacagcct gtcacaagac
      721 gtaacccaaa cgcaccaaca gttaaccaac acccaaaaac gggttgcaga taacagccag
      781 caaattaaca cgctcaataa ccatttcagt tcgctaaaaa acgaagttga tgacaatcgt
      841 aaagaagcca atgcgggaac tgcatctgcc atcgctatcg cctcacaacc acaggttaaa
      901 accggtgacg tgatgatggt gtcagcggga gcgggaacct tcaacggtga atctgcggtg
      961 tctgtcggaa catcatttaa tgccggaacg catacggtac ttaaagccgg tatttctgcg
     1021 gatacacaat ctgatttcgg cgcaggtgtc ggcgtgggat attcgttcta atatttcaat
     1081 cctcaatata aataagagca aggaagcttg ccgggttcac ctcttcatta atttgtacat
     1141 tatttaaggt taacaatgat gaatagctcc attaaatcgt tttccctgct ggcggttata
     1201 ttactggctg gctgtagttc acccacttcc cgcatcgcag attgccaggc gcagggcgtc
     1261 agtcatgaca cctgttacct cgcagaacag cagcgtcagg cggctatttt aagtgcatcc
     1321 gaggcacagg catttaaaaa tgcagaagcc gcacaacacg cccaggcggc aaagaaagcc
     1381 atttataaag gatttggcat gacctttaga atgagcagta aaaactttgc ttatctcaat
     1441 gattcattat gtgcaattga tgaagacaat aaagatgcca ctgtttatca gtcaggtcta
     1501 tataacgtca ttgtttatca tcacacagga aaagtcgcct taatgaaaga aggccagttt
     1561 gtgggttatt taaaatgaag gagcaaagga aaatacccct gacgcatatt atgattatcg
     1621 gtgcgtttat ttttgccttc ttgcaagtag tattattagc ctccctggtt cacgctgtga
     1681 atgttaacaa cgaaatccag gaaggcttat ttcagtcggg gcgcattatg gtagaaagtt
     1741 tgcagcatat tctttcggtg caaacgggga ttcactgatt ttcaccccgc ccgatgatga
     1801 cagcagccgg agagattttc gataatcggg cagtcggcgc tgtcatcacc ggggcaggca
     1861 ttcgccagcg ccagtagctg gttgcgcata gattgcagtt cttcgatatg ccgttcaatc
     1921 tccgccacct tctccagcgt gcgacgtttg acgtcggcac tgtgacgctg cgggtcgtta
     1981 aacagattca ccagctcgcc gctctcttcc aggttaaagc ccacctggcg cgcctggcgc
     2041 agtaaggtca attcgttgag atgctgctgc gtgtaggttc gataaccatt ttcgctgcgc
     2101 atcggcggcg tcaccagccc cttctcttca tagaagcgaa tggctttgct ggtcaggccg
     2161 gtaatttttg ctacatcgct aatgttcatc gttcgcgcaa cgcctcctgt gctttgttaa
     2221 agggtttcaa cagataatcc agcacgcttt ttttgccggt gcggatatcg accgtcgcca
     2281 ccataccggg gaatatcgga aactgctgtt tgcttttgtt ttccagatgg ttgctgaaag
     2341 tgcgaatgta cacccgatag taataaacat cgcgccgcac ttcatcctgc acggtgtctg
     2401 gtgatatcac cgccacttct cccggcaggc cgccgtagat ggagtaatcg taggcagtta
     2461 ttttcaccag cgatttttgc cccgggtgga taaacgccac atcgcgcggg gagatttttg
     2521 cctcaatcag cagttgctca tccagcggca cgatggtcat cagtttacca ccgggggcga
     2581 tgacgccgcc aaccgtggtg acatcaatat cctgaacaat gcctcgcacc ggcgcggtaa
     2641 agttgagcct ggtgagagag tcttcccgtc cacggatcac cgaacgctgg gtttccactt
     2701 ccgcattggc ttttgctaac tcttcgcgcg cctgaacgta gtattgcgtg cgcacgtcgc
     2761 tgagtttgtt ttccagttca ttggcctggc gttgcaggcg cagtacttcg acgctgctcg
     2821 ctgccccctg ttttaccagc ggctgggtca tcgccagttc gcgattgacc aggctaatcc
     2881 cctgcgtcag cccggtggtg gctttgttca acccgtcgcg gcgagtgttg tacaacgccg
     2941 tttccgactc gataagttcg gggaatttat tcagttcgtc agggaagacc agcggtttat
     3001 tgctcacttc cgcccgcagc cgcgccgagg ttgccagcgc cgcgtacagc cgcgacatcg
     3061 cttcctggac gtcggattcc gttttggtgc ggttaagctg cgccagcggc tggccctgtt
     3121 cgacaatatc gccaactttc acgtcgagat gatacagaat tccgccttcc agcgactgca
     3181 ccacctgttc gcgcccggaa ggttctaccg tgccgctgcc ggtggtgact tcatcaagct
     3241 ggaagtacca cgcccagccg agaaaacaga gcagcatcac agcggtaacg cgtaccgcca
     3301 gcgccgaacg cggcaggcgg ggttcagtca gaccacggtt atattgcagt tgtccgttca
     3361 tgccgcagac cccttattct tctcctgcaa caccacccgt cgcgctgtgg gctcgccctg
     3421 ttcacgcaaa atggcctctt tcgcgccgtc catcacaatc cgcccctgat ccatcacgat
     3481 tatcctatcc accagttgca gcatcgccgt gcggtgagtg gcgataatca gcgtgcgatg
     3541 cccaatccac tgtttcagtt gttcgattaa atacgcttct gccatttcgt ccagtgaggc
     3601 gctgggttcg tcgagcagga cgatattcgg ctgagagatg agtagccgcg ccagcagcag
     3661 cgtttgccgc tgaccaccag agaggccaaa tcccccttcc tgaatcatgt agttgaggcc
     3721 gtttttctgc ttctgcacaa agggcagcgc tccgctgagg gttaacgcgc gttcgatatc
     3781 ttcatcgctg gcttccggca tccccatggt gaggttttcc cgcagcgaac caaagaacag
     3841 tcgcccggtc tggctgagta gccccatgtc tcgccgcaga tctgccggat caagctgccc
     3901 gatactgatg ttatccagca acacctgccc ctgctgggcg atacgcattc cggcgagcat
     3961 ttgcagtagc gtcgatttac ccgcgccgtt gcgcccgaga atggcgattt tctcaccagc
     4021 ctgaatgttg agtttgccaa tcgctacatc ggcaatcttc tcttcttcgt cgtaataaaa
     4081 caccgcgttg ctgaactgat aattaccgtg cagcaccgct ttatgcacca gtttgccgtg
     4141 ttccggctga tccaccgggc gcttcatcag ttcatcaagg ccgttacgtg ctactttcgc
     4201 ctgctgccag cgcgagagca cgccggatat ctgcgacagc ggcgcaatgg tgcgcgaagc
     4261 caggatcgtc gtcccgacca gcgcgccggt ggtcatgtcg ccgttcatca ccataaagca
     4321 gcccaccagc agcaccacca cgtagacgat ggactgcact tcctgggtcc aggtgagcag
     4381 cagcccggtg agaaaacgtt gcttcatgct gatggacgag gccacgtcgt tggtgtggtt
     4441 ccactgattc tgaaaacgct gttcggcgcg cagcagttta atatcttcaa tcgactgcac
     4501 ggcttccacc agcgtagcgt tgcgtaccgc tgactcgcgc attccttcgt tcgccagccg
     4561 cgccagcggg cgttgcacca gcaggccggg gatcaccagc aacggcagcg ccagcaaaac
     4621 caccagcacc agccagccgc cgatcatcca caaaataaag acaaacagca ggaagaatgg
     4681 caggtccgcc accgcgccga tggtggtgga cgtaataagc tcccgcactg attccagttc
     4741 gcggatctgc gagataaacg atccggtgga tttcgatcgc acgttgtttt tcagccgtag
     4801 cgcatgacca aaaacgcgat ccgaaatacg caggtctgcc cgctttccga tcacatcaga
     4861 tagatgcgta cgcaccatgc gcatacagaa ctcaaacagg atcgccatca tcaccccggc
     4921 gaaaagcacc catagcgtcg ggtacgattg cgccggaacc acccgatcgt aaacctgcat
     4981 cgagaaaatc atcgccgcca gcgccagcac gttcgccacc agtgacgcca gcataatatc
     5041 gccgtagcgc cgccagtctt ttagcgaaag actccagaac cagttcgcct gatacgggcg
     5101 aatatagtca tccacccgcg cgtcagggat ggcgctgaga gggcgcagca gcgccagttc
     5161 gacgatttta tcttcaatat ccgcgacgtt aagtgtagtt tccagccctt cgtcaccgct
     5221 gaactgaacg ctgatattgt catgattgtc gcgacgggtg atcacgccaa tctgctgatt
     5281 atcaaacacc gcgatcagcg gcagacgcca ggggttaagc gaatcggcgg aaaactccac
     5341 caaccgcaac cccatcccta actgccgcgc catgtcggtg agtaactcct cccgcttact
     5401 gtcgcgctcc cagttgacgg tcgcgcgcac attctcctga gaaaaatcga gccgataata
     5461 gcgcgccacg gcgatcatcg cctccagcca ggcttccacg gactgggcat tttttttcat
     5521 tatctacacc accatcaact gatgattcgc cagcaaggtg gcgaggtcgg tgtgcacgcc
     5581 cgtcagcgtg accacgttag ttgtcgcaaa agtgccgccc gtgccgtcgc ggtcgatctg
     5641 cacgatggtg tcgctgccgc tctgggtgac tttgacaaag tcaccgatgt ttccggcctg
     5701 cgcatccagc gttgccacgc cgttgacgta gctggctttg ccgttgccgg tgtagccgct
     5761 gccctgtaac agttcacgaa tatcaatgcg atcggtgtcg gcggtgcctt cccatgtccc
     5821 taccgtaaag ccattcacca cgtctgagcc gttgccgcca gtggcgtcgc tggcgttgag
     5881 cagtttatag agcaaggtgt cgtggccgcc gttgccgatg ttgagcgtgt cgttgccgcc
     5941 gcgcccttcc agttggttat cgccgctgct gccggtcaga atgtcgttaa aattcgagcc
     6001 ggaaatgcct tcgatattgg taaatgtcga ggtgttaaaa ccggtggcct gcgcggcagt
     6061 gctgcttaag ttagccgtca cgcccaccgt cgaaagccgg aaatcaacca cgtccatccc
     6121 gccgttcgcc agccacgttc cggtgccgga gctgtacacc cagccgccgg aaccgtcgta
     6181 agtgtcggtg cctgcggtgg cgaagaaggt gtcgttatag ccggtgccga cgaacttgcc
     6241 gttgttgctc gcggtacccg cttcagcgct gagatcgtaa gcgtgagtgg catcggtggc
     6301 ctgtaaaccg ccccagctgg tgttgaccgt ctggtcgata ttgctggcgg tggtgccggt
     6361 ggatttgatc agcaccgcgt tgtaggtttc tccggcattc agcccgtaga agttgaccag
     6421 cgccgaggtg tcgttaaccc ccatacccga ctgcgggttg atgatttgcg tggcgaccag
     6481 cactcccgcc gagttgtaca gcttcacggt attgccgtaa tagacgttaa tgccgttggg
     6541 atcggtgatg cgcaggtgta gcgaagtgcc gtagctcacc gtgttggtgt tgcggctcag
     6601 gaacacgctg cccgactgtt tggacaccag cacatccacc gcgccgtccc agtcgtagtc
     6661 cattgccgcc acgccgctgg tggtaccgct ctggctgccg ccgagggccg attgcgtcca
     6721 gttgctggcg ttgctgacgt tagtataaag atacgactgc ccggtctggg cgatacgggc
     6781 gatatccatc aggccgtcgt ggttccagtc caccgccagg ctaaactggc tggcgtaggt
     6841 ggttgccgtt gcgcccaccg ccaccgggct acccagtacg ccgttggtgt tgaataacag
     6901 cgagccgtag ttagctcttc cctggctggc gggcaggaag agatccatat cgccgtcacc
     6961 atcgaaatcg gcccaggtca tggaaacgct gctgctcata gcgccactgc ccgccccgct
     7021 caggaaggta ttggtggtgt tctgccccca gacaaacgtc ccattcccct ggttgatgag
     7081 cgaagacagc gtgtaatagt tgttcaggtt ataggtgtgc tgaaccaggt cgatcctgcc
     7141 gtcattgttc agatcgacgc cagaaccttc gttgagcgaa agataccccg tcaccgcccc
     7201 gcccaccgtg gcgctaccgc cgctgttcga cgtggtggag ttgcctacca gcgtgcctgc
     7261 gttgttccac aggaaggtgt tggagtccgg cccgccagcg tcaccaatcc agaagtcgag
     7321 atagccgtcg ccctctttat caaatgccac aatcgagccg taccacgtca gggtgccgac
     7381 ggtgacctga atagcgctgg aaaaggtgcc atcgccgtta ttggtaaagc cgttaatata
     7441 gcccgtaccg tagtcatcac gggtgataaa cagatcgccc gtgccgttac ggtcgtaatc
     7501 ggcaatggag ctggtggcgt agttgttgcc gctctgatac agcgtcagcg cggtcttcga
     7561 ccaggtgagt gggtcagtcg attgcattac ctgctggttt gccagcaccg tccacatccc
     7621 gtgcgagtcg aggccgtagg tcagccccca ggcggtggtt ttgctggcgg tagtccaggt
     7681 cggtgtgtaa tcaatcgccg cgttaaccgt caccgtgccg ttgctaatat tggcgttatt
     7741 gccgttaccc gccgaacttt ttacctgcgc agtaacggta taggcggtcg cggaaactgt
     7801 cagcgcatcg gtatccggca actgtacata ccaggtgttg tgcgccggat cgaccactac
     7861 cgcgccgccc ggttcagagg tgtaggtttt gccgttgatc accacttcaa catactgccc
     7921 actggcaagc gcggcggtga tcaccccgct aataatcggc gtagtatcgc gcgtggtctg
     7981 gctggtgatt tgcgccagcg tggtcggaat agaggtatcg accgtcagga caaaatcact
     8041 ggaggaggtg atattccccg ccaaatccac cactcgcgcg ctgtaggtat aggcaccgct
     8101 caccagcccg ctgtcgctgt aggtccagtt cagagcgccg accatcgtca ccgcccctaa
     8161 cagcagcccg ttacggtaga ggtaaaccac ttcaccgctg gcaagcggcg cggaaagtac
     8221 accgttcagc agcggcgtag tgtcgtcggt ggcctgcgaa ctgcttaatg tcccctgccg
     8281 ctgcccgaca tcatcggtat acgacacaat cgttggtgtg gcggtggggt taatcgtatc
     8341 gaccaccacc gttttcgaca ccaccgggcc ggtgttcccc gccagatcca gcacccgcac
     8401 ctgataaacg tagctgccgt cggtcagcgt gcggctgtcg gtgtaagtcc actgtgtacc
     8461 gttggtggtg agcgttgtcc aggtcgcgcc gctatcaagg ctaatctgcg ccacttcgcc
     8521 gctggcaagc cccgccccca gcgaaccggt cagcgtgagc atggtgtcgc tggtgataaa
     8581 gtcagttgcc gaactccccg tgtcggtgct gatgctggcg atggtgagca cctgcgccgg
     8641 gttaacggta tcgatcagcg ccgactggct gctcgttgcg ccaacgttgc ccgccagatc
     8701 gaccacccgc acctgccagg tggtggtgcc gttggtgagg gttcgtccgt caacgtaact
     8761 ccagttcagg ctgtctgcgg cgacggtcac gttcacccag gttgcgccgt tgtcggtact
     8821 gatttgcgcg aactcattag cgccgagcgc cgcgcccaat acgccgcgca cggtaagcgt
     8881 ggtgtcgctg gtaataaaat cgctgctgct ggttccggtg tcatcgctga taccggtaat
     8941 ggtgatactt ttcgccgctt ccgggctggt ggtgtcgacc accacgtttt gcgttgcggt
     9001 ctgcccaacg ttccccgcca gatccaccac ccgcaccgtg tagttccagg tgccgtcagt
     9061 aagcgtgtga ccatcggcat agctccagct ggtgccaacg acggttaacg tagtccaggt
     9121 gacgccgccg tcgaggctga tttgcgcgaa ctcaccggca gaaagcgtcg cccccagcgt
     9181 gccgctcacc gcaagcgtcg tgtcgctggt gacaaaatcg ttagtgatca agcccatatc
     9241 ggtggtgatc gcgctgatgg cgatggtttt caccgcggga tctggcgcgg tagtgtcgat
     9301 caccacattc tggctgtcgg tcgcgccaac gttgcccgct gcgtcaatca cccgcacctg
     9361 atagagatag ttgccatcgg tcagcgtgcg gctgtcgtta tagcgccagg tcgtgccggt
     9421 aacggtaagc gtggtccagg ttgtgccgcc gtcgatactt atttgcgccg tctcgttact
     9481 ggcaagcgcg gcggtgagcg agccgttgac cactaccgga ctgacgctgg tgataaagtc
     9541 gctggcgcta aggccagtgt cggcttgcag ggaatcgata ttaatgccca tcgccggagc
     9601 gctgagatcg acggtaatgg tgtgattact gctaccggtg ttgccaatgg cattgctcac
     9661 cgaggcgtta atcacgtagg cgttgccgtc cgccagcgcg gtgacatcgg cgctgccgag
     9721 ggtgtagctc cagctaccgc ccgtctgcac cgtggtggtg taggttttgc cgtttaaggt
     9781 cagggtcagc gtctgcccga cgggcgcggt ggtggtgccg ctgatcacca gcgatgagcc
     9841 gtgttccgct gcattcacca catcgtcgcc cgcaaaggtg ttgatggtga cagtaggtac
     9901 atcgccgttc agcgtcacgc cacggctggc gctgccgggg tttccggcct gatcgctaac
     9961 cgtcgcgcta atggtgtagc tgccgtcgct taatccggca aactgctgcg ccggaataaa
    10021 caccgaccag cttccgccgc tgccaaccgt tgcgctccag gtttgcccgt taaaggtaac
    10081 ggtcaccgtc tggcccactt cggcggtggt ggtgccgcta atggtctgcc cggcaagctg
    10141 ctcggcgttg ttgataatat cgtcacccgc caccgtggcg atggtgaccg ttggcgcaac
    10201 ggtatcgacg gtcagggcgt gcgtggcctg tccggggtta ccggcgcgat cgttcacgct
    10261 ggcggtaatg gtctgcgcgc cctgcccgag cgcggccaga tccaccgcag gcacgttcag
    10321 cgcccaggta ccattggcgg caaccgtggc ggtataggtt ttgccgccaa aggtgacggt
    10381 gaccgtctgc cccacttccg cgctggtcga gccgttaagg gttaacggct gctgctgttc
    10441 ggcggcgttg aggcggtcgt ccgtcgaaac ggtgttaatg ctgataaccg gcgcggtgag
    10501 atccaccgcc acgctgcgcg aggccgtggc gctgtttccg gcactgtcct gagcggaggc
    10561 gctaaccgtg tagctggtgc catcactgag tgccgcaaca tccgccgcag gcacgttcac
    10621 gctccagctc ccgttgctct gaatggtggc gctgtagcct ttgccgttaa gcaacacggt
    10681 caccactgta cctgtcgcga aattagtgcc agtgccgctc agggtcagcg cactgcccgc
    10741 ttcggcggcg ttaataaggt tatcgccgct gatagtgctg accgagagcg acactgccgc
    10801 cgtgttcacc tgtacgttgt gcgtctgcgt gctgctgttg cctgcgctat cggtaatggt
    10861 ggcgctgatg gtcaccgtgc catccgcaag gccggaaatc acgctggcag gcacgccgac
    10921 gctccagttg ccgctggcat cggtgctggt gacatactgc tgcccggcga tggtcaccac
    10981 caggcggtcg cccgccaccg cgcccgtggc ggtgccgctg ataatctgcg cctgaatgtg
    11041 ttcgacgttg ttaatcacat catcgcccgc cacggtgtta aaactgatca ccggcgcagt
    11101 ggtatcgacg gtcacgcctt tgctggcgct gccaaggtta cccgccagat cgctgaccgt
    11161 ggcggtcagg gtgtagccgt tattagcaag tgcggttaag tcgctggcgg gcagcgttaa
    11221 actccagctg ccgtctgtct gcaccgtggt ctggtagtta gtgccgttaa gcgtgacggt
    11281 gagcgtctgc ccggtttgcg cggtgctggt gccggacacc gttacgcccg ccgccgcttc
    11341 gctggcgttg atgatgttat cgctggcaat ggtgttgatg gtcaccgttg gcgcggcgct
    11401 atcgacgctg tattcgtgcg ttgctgtggc gctgttgccg ttgacgttag tcacccgcac
    11461 ctgggcgctg gcatcgccat cacgcagggc cgccaaatcc gccgccggaa cggtggtgct
    11521 ccagctaccg tttgccgcca ccgtggtggt gtaggttttg ccgccaaagg tgacggtgac
    11581 ggtttgcccc gcttcgacgc cggacgtgct gccggagagc gttaacgccg cgcctttttc
    11641 ggcggcgtta atcgcatcat cgctggtgat gctgttcatg ctgatggcga cggcggtgag
    11701 atcaacagtc agcggatggg taatgctggt ttgcgttccg gcactgttcg ccccgctaac
    11761 ggtaatattc agcgttcccg caggccagtt actgacatcc gtcgcaggaa cgccgacgct
    11821 ccagctaccg ttcgccaaca ccgtggcggc gtaagtttgg ttgttaatca ccaccgtcaa
    11881 cgccgtgcct gcggcaaagc cggagctgct gccggtgatc acctgcgcct gagcgtgttc
    11941 gatgatattg accacatcat cacccgcgac ggtatccacc cgcaggccag gcagactggc
    12001 atcgatggtg atatcgcgcg tgccgctacc ggtgttgcca acggcgttgg tcaccgaggc
    12061 attaaccgtt aactcaccgt tacccagagc ctgccagtcg gcggcaggta cgctcacatt
    12121 ccagcttaaa tcagcctgga cggtggcggt gtaagtttta ccgcccagtt caacggtgac
    12181 ggtgctgccc gccgccgcac cggttacctg gccgctgata gtttgcgcgc tgcctgcctc
    12241 tgaagcgttg ataatatcgt ccgtcgccac cggattaatg gtgatgcccg gcagcgcggt
    12301 attaacctgc acgttatggc tggcggtgct gctgttaccc tgagcattgg tgacgctcgc
    12361 cgtcacgctg taggtcgctt cgcccagcgc actgaccgcc gaggcaggca cggtaacact
    12421 ccagttgccg gaggcatccg tggtggcgct gtagttttgt ccgttcaggg tgacggtgat
    12481 cgccgtaccc gcaggctgat cgctggtgcc agaaagcgcc agatcagatc ctttttccgt
    12541 cgcgttgatc acatcatcgc ccgcgatggt gttgatgcta atcactggcg cgctgaggct
    12601 gacggtcacg gtgcggctgg cgtcgtcgct gttgcctgcc cgatcgctga cgctggcggt
    12661 gatggtctgc gcaccgctac ccagcgccgt aacatctgcc gccggaacgc caacgctcca
    12721 gttgccggag gcatccaggg tggtggtgta ggttttgccg ttgagcacaa cgctcaccac
    12781 atcgcccgct tccccgccgc tactggtgcc ggagatcacc agcgcctgcg catgttcggc
    12841 atcgttgatg atgtcatcgc ccgccacggt gttaatggtg agaaccggcg cggtggtgtc
    12901 taccgccaga ttatggctgg cgctgccggg gttgcccgct ttgtcattga ccgtggcgct
    12961 gacggtgtaa ttgcttgcgt tgagcgcgcc cagggctgaa gtgggaacgc tgacgctcca
    13021 gctaccgtcc gcctgcacag tgccggtgta ggttgcgccg ttaagggtga cggtcaccgt
    13081 ctgcccggtt tcggcggtgc tggtgccgct gatggtgagc gggcttccgg cttctgcggc
    13141 gttaaggata tcgtcgctgg cgatggtgtt aatggtgagc gtcggcgcgc tggcgtcaac
    13201 gctatacgca tgggtcgccg aggcactgtt accgctcgcg ctgctaacgc tggcctgcac
    13261 attcgccgcg ccctctggca gagttgccag atctgccgcc ggaacgttta cactccagct
    13321 cccgttggcg gcgacgcttg cagtgtaggt tttaccgcca aaagtgacgg tgacggtttg
    13381 cccgctctca atcccgctgg tactgccgga aagagtcaga ttggtgcctt tttctgcggc
    13441 gttaatcacg tcgtcgctgg caacggtgtt gatgctgatt gccaccgccg ttaaatcgac
    13501 ggtgaacgga tggctgacgc tgactgggtt attggcgctg ctttgcccgt ctacctccac
    13561 ggttaacggc cccgcaggcc aggcgctgac gtttgccgcc ggaatgccaa cgctccagct
    13621 tccgtccgcc tgcaccgtgg cggaatacgc cacactgttg atggtaaccg tcagcacagc
    13681 acctgcattc aggccgctac tgccgccggt gatcaccagc gcctgcccgt gctcgatgct
    13741 gttgacgata tcatcgcccg ccacggtatc tacgcgcagc cccggcaggt tggcatcaat
    13801 ggtgatatcc cgcgtgccgc tcccggtgtt gccattagcg ttggtgaccg aggcggtaat
    13861 ggtcaaatca ccattgccca acgcctggag atcggctgtc ggaacgctga cgctccagct
    13921 taagttgctc tgtaccgtag cggtgtaagt gttcccgccc agcgtcacgg tgaccgtatc
    13981 gccagcggcg gcgcgagtca ccaccccgct gatggtttga tcggcccccg cttcggcggc
    14041 gttgataata tcgtcgcctg ccacggtgtt gatggtgacg tccggcaggc cgctgtcgac
    14101 cagtaaattc gcctggctgc tggcggtgtt gcctgctgca ctggtggcgc tggcgctgac
    14161 cgtatagttg gcctgaccta atgccgccag atcgctcacc ggcacggtta aggtccagtt
    14221 gcccgatgcg tcggtggtgg cgctgtagtt gtgaccgttg agcgtgacgg tgacctgcgc
    14281 gcccgtcgcc agccccgtac tgccgccgct gatggtcaac ggctggcctt tttcatcggc
    14341 gttcaggatg ttatcgccgc tgatggcgtt aaaggtaatg gtcggcagcc cggtattgac
    14401 cgtcacctga tgggtagcgc tgccgctgtt tcctccggca tcggtgacgc tggcattgat
    14461 ggtcaccgtg ccattcgcca gtgccgagac gacgcttgcc ggaacgccga cgctccagtt
    14521 gccgctggca tccagcaccg tggtaaaggt atttgtgccg atagtcaccg tcacggtgct
    14581 accggttgcc gctccagtgg cggagccgct gatgatctgc gcctgggcgt gttccgtcgc
    14641 gttgatcaca tcatcgcctg ccaccgtgtt gatggtgacg accggaacgg acgtatccac
    14701 cgtcaggttg tgattaaccg aggccgggtt tccggctttg tcgctcaccg ccgcgttcac
    14761 ggtgtagttg ctggcggtca gggtgcttaa gtcggctgac ggtacgctga cgctccagct
    14821 gccgtccgtc tgtacggtgc cggtgtagtt tgtgccattg agcgtgacgg tcaccgtctg
    14881 ccccgcttcc gccgtgctgc tgccggtgat ggtcagagcc gctccggctt cggcggcgtt
    14941 aagaatatca tcgcccgcaa tggtattaat ggtcaccgtt ggcgcgctgg catcaacgct
    15001 gtaagcgtgg gtcgtggtgg cgctgttgcc gttaacattg ctgacgctgg cctgtgcgct
    15061 ggcatcgcca tcacgcagag ccgccatatc tgccgccgga accgaggtgc tccaggaacc
    15121 attcgcagcc accgtggcgc tgtaagtttt gccgccaaag gtgacggtaa ccgtttgtcc
    15181 ggcttcaacg ccagaggtgc tgccggagag cgttaacgcc gcgccttttt cggcagcgtt
    15241 gatcacatca tcggcggtaa tggcgttgat gctcaccgcc accgccgaga gatcgaccgt
    15301 caccggatgc gtaacgctta ccggatttcc ggcagaggtg ctaccgctcg ccgcaatcgt
    15361 caccgatccc gcaggccagg cgctgacatc caccgccgga acgccgacgc tccaggtgcc
    15421 atccgccagc accgccgcaa cataggtttg cccgttaatg gtcagcgtga cgttgctgcc
    15481 cgccgccaga ccggagctgc tgccggtaat caccagcgcc tgcccgtgct cgataatatt
    15541 aaccacatca tcgcccgcca cggtgtcgac ccgcagaccg gggagattcg cgtctatggt
    15601 gatttcgcgc gtgccgttac cagtattgcc tacgctgttc gtcaccgaag ccgaaatggt
    15661 cagttcgccg ttgcccaact cctgtagcgc ggaggccgga acatcgacgc tccagcttaa
    15721 attcgcctgc accgtggcag tgtaagtcgc cccgccgagc gtgacggtca ccgtgtcgcc
    15781 tgccgccgca cccgtcacct gcccgctgat ggtctgttcc acgcccgctt cggcggcgtt
    15841 aataatatcg tccgttgcca ccacgttaat ggtgacgccc ggcagcgcgg tattaacctg
    15901 tacgttatgg ctggcggaac cgctgttgcc atcggcgtca gtggcggctg cggtcaccgt
    15961 gtaggtggct tcaccgaggg cgctcacccg tgacgccgga acggtcacgc tccagttacc
    16021 ggaagcatcg gcagtggtgg tgtaattttg cccgttgagc gtaacggtaa tctgcgtgcc
    16081 cgcaggctgg ttgctggtgc cggtaatcgc cagatccgcg cctttctccg cggcgttgat
    16141 gatgtcatcg acggcaatgg tgttaatggc gagcaccgga gcgccgagcg ccacggtgac
    16201 agtgtgcgag gccgtgccgc tgttgcctgc ggagtcggtg accgtagcgg taatggtcac
    16261 atcgccctgc gccagcgcgg aaatcacgct ggcaggcacg ccgatgctcc agttgccgtt
    16321 ggcgtccagc acggtggtat aggtggtcgt gccaatcgtc acggaaaccg tattaccggt
    16381 agtcgcgccc gttgccgagc cgctgatgat ctgcgcctgt ccgtgttccg tggcgttaat
    16441 gatgtcatcg cccgccaccg tgttgatggt gacgaccgga gcggcaaggt ccaccgtcag
    16501 attatgcgtt gccgaagccg aatttctggc tttgtcgctg accgaggcgt taacggtata
    16561 cgagctggcg gtaaggctag ctaaatcgcc ggttggtacg ctgacgctcc agctcccgtc
    16621 tgcctgcaca ttgccgctgt aattaacgcc gtttagcgtt acggtcaccg tctgcccggc
    16681 ttccgccgtg ctggtgccgc tgatggtcag cgccgatccc gcttcggcgg cgttaagaat
    16741 atcgtccgtg gcgatggtgt tgatggtaac ggagggcgca gtggcatcaa cgctgtaagc
    16801 atgcgttgcc tgggtgctgt tccctgcgac attgctgacg ctggcctgga cgtttgctgc
    16861 gccgtctggc aaggttgcaa catcgacggc agggatcgtc agcccccagg tattatccgc
    16921 ggcaaccgtg gtggtgtagc ttttgccgcc gaagataacg gtgatggttt gccccgcttc
    16981 cacgccggag gtggtgccgg agagctgcaa atcagagcct ttctcggcgg cgttaatcac
    17041 atcgtcagtg gaaagggtgt tgatggtgat cgccacggca gcgagatcga ccgtgaccgg
    17101 atggctaatg ctggttgtgg ttccggcggt gttggtgcca gagacggcaa tattgaccgt
    17161 ccccgcaggc cagtttgtga catctgccac cggaacacca acgctccagc taccatccgc
    17221 taaaaccgtt gcgccgtagg tgacgctatt gataaccacc gtcaacgccg cgcccgccgc
    17281 cagtccgcta ctgctaccgg tgatcaccaa cgcctgagtg tgctcgatgc tgttaatcac
    17341 atcatcgcct gccacggtat cgacgcgcag acctggcagg ttggcatcta tcacgatatc
    17401 gtgcgtcgcg gtgccggtat tatttgcgga attggtcaac gaggcgctga tggtcagttc
    17461 accgttaccc agcgcctgca aaacgttcgc cggtacgctg acgctccagc ttaaatctga
    17521 ctgcacggtg gcgttatatt gattgccgcc aatggtgaca atcaccgtat tcccggcaac
    17581 cgccgttccc gtgacctgac cgctgatggt ctgcgccaca acgatttcag cggcgttgat
    17641 aatgtcatcg ccggagacgg tgttaatggt cagcgtcggg ccgctaagat caacggtcac
    17701 atcgtgagtc gtgctgtcgc tgttgcccgc gcgatcgctc aggctggcgg tgatggtctg
    17761 gctaccggtc gccagcgccg tgacatccgc cgccggaatg cccacgctcc agttaccgga
    17821 ggcatccagc gtggtggtgt agtttttacc gttcagcgtg acggttacca catcacccgc
    17881 cgccgcgccc gtactggtgc cgctgaccac caacgcctga ccatgttcga tggcgttgat
    17941 aatgtcatcg cccgcgacgg tgttaatggt gagatccggc gcagtgatat ctaccaccag
    18001 cgcgtggtcg gcgcttgccg ggttacccgc tttgtcgctc accgtggcgg tcacggtgta
    18061 actgctggcg gttaatccgc ttaaatccgc tgccggaaca ttcacgctcc aggtgccatc
    18121 cgccagaacg gtggtctggt acgtgttgtt attgagcgtg acggtgagcg tctgcccggc
    18181 ctgcgccgtg gtagtgccgc tgatggtgat ccccgcgcca gcttcgctca cgttaaggat
    18241 atcgtcgctg gcgatggtgt tgatggtcac aagcggcgcg gtggcatcaa cgctatacgc
    18301 gcgatcggcc tgggcgctgt tgccgttaat attactgaca ctcgcctgca cgttgcccgc
    18361 gccgtccggt agcgcagcga gatcggcagg cggcacattc accgtccagc taccgttagc
    18421 ttccactgtg gtggtgtaat ttttgccgcc aaaggtgacg gtcaccgtct gcccggcttc
    18481 gacaccgctg gtgctgccgc ttacgaccag cgtttcgcct ttttcgacgg cgttgatcac
    18541 atcatcacca gagagagtgt taatggtgat ggcgacccca gccagatcga cggtaaccgg
    18601 atgggtaatg gtggaggttg ttccggcact gttggtgccg cttaccgtga tattcaccgt
    18661 acccgctggc cagttgctga cgtctaccgc cggaacaccg aggctccacg tgccgtcggc
    18721 taataccgtc gcgccataag taacgttgtt aatctcgacc gttaacgccg ttcccgccgt
    18781 taatccgctg ctgctgccgg tgatcaccag cgcctgattg tgctcaatgc tgttaataac
    18841 atcatcgccc gccacggtat cgacccgcag gccaggcaga ttggcgtcga tcgtgatatc
    18901 gcgcgagccg ctgccggtgt tgccgacgcc attggtgacc gaggcattaa ccgtcaggtc
    18961 gccgttgccc agcgcctgaa tatccgccgc cggaacgtcc acactccagc ttaaattaga
    19021 ctgcaccgtg gcggtgtagg tgttgccgcc cagcgtaacg gtaaccgtat ccccctgcgc
    19081 tgcgcccgtc acctgcccgc tgatggtttg cgcatttccc gattcggcag cgttaataat
    19141 atcgtcagtc gccaccgcgt taatggtaac ggcaggcaag gcgctgttga ccagcacatt
    19201 atgactggcg gaattactgt tgcctgccgt atcagtgacg tttgccgtta ccgtgtagtt
    19261 ggcttcaccc aatgcgctaa ccgctgacgc tggcaccgtg gcgctccagt tgccgttgct
    19321 atcggtagta gcggtgtaat tttgcccgtt cagcgtcacc gtaatggtgg tgcccgcagg
    19381 ctgattactg gtgccggtga tttgcaggtc cgcgcctttt tccgtggctt taatcacgtc
    19441 atcggtggcg atggtgttga taccaatggt tggcgcggcg agattcacgg tgaccgtgcg
    19501 gctggcgtca tcgctgttgc ctgccgcgtc ggtaattgcc gcagtgatag tttgcgggcc
    19561 gctgccaagc gcagtgacgt cagccgccgg aacgccgacg ctccagttgc cggaagcgtc
    19621 cagcatggtg gtgtaggttt tactgtttag tgtgacggtg atgacatcac ccgcttcgcc
    19681 gccagtgctg gagccggaga tcaccagcgc ctgtccgtgt tcggcggcgt taataatgtc
    19741 atcgccggag acggtgttga tggtcagcac cggcacggtg agatccaccg ccagcccgtg
    19801 gttagcggac gccgggttac ccgctttatc gcttaccgag gcactaacgg tgtactggct
    19861 ggcggtcaga ttgctgagat ccgccgtcgg taagctgacg ctccagctgc cgtccgcctg
    19921 gacggagccg ctgtaagtca caccattaag cgtgacggtt accgtctgcc ccgcttcggc
    19981 ggtgctgcta ccgctgatgg ttaacggatt gcccgcctcg gcagcgttca gaatatcgtc
    20041 ggtggcgatg gtgttaatgg caagcgtcgg ggccgtggca tcgacgctgt aggcgtgggt
    20101 tgccgaagcc gtgttgccgt taatagtgct gacgctggcc tgcacggtgg cgtcgccgtc
    20161 gcgtaacacg ctgagatcgg cggcgggtac ggtggttgtc cagctaccat cgcccgctac
    20221 ggtagcggtg taggttttcc cgccaaaggt aacggtgacc gtttgcccca cttccacgcc
    20281 ggaggtgctg ccggaaaggg ttaaatctgc ccctttttcg gcggcgttaa tcacatcgtc
    20341 gccggaaacg gtgttaatgg aaatcgccac cgccgccaga tcgacggtga ccggatgggt
    20401 gatggtggat gttgttccgg ctgtgttagt gccacttacc gtgatattca ccgtacccgc
    20461 aggccagtta ctcacgtctg ccgccggaac accaacgctc catgtgccat cggctaatac
    20521 tgttgcagcg taagtgaccg tgttaatcac caccgtcagc gccgcgcccg ccgccagccc
    20581 gctgctgcta ccagttatca ccagcgcctg agcgtgctcg atgctattga ccacatcatc
    20641 gcccgccacg gtgtcaacgc gcagacctgg caggttggcg tcgatagtaa tatctcgcga
    20701 accgctgcca gtattgccaa cgccgttggt caccgaagcg ttaaccgtca gattgccatt
    20761 gccgatggcc tgaatatccg ccgccggaac gtccacgctc cagcttaaat tcccctgcac
    20821 ggtagcggtg taagttttcc cgccaagcgt tacggtaacc gtatcgcccg ccgccgcccc
    20881 cgtcacctgg ccgctgatgg tttgcgcatt gcccgattcg gcggcgttaa taatatcgtc
    20941 ggtcgcaact gggttaatgg tgacgccagg cagcgcggta ttgacctgca cgttatggct
    21001 ggcggaattg ctgttgcctg cgctgtcggt gacgttcgcc gtcaccgtgt agctggcttc
    21061 gcccagcgca cctactgcgg aagctggcac cgtagtgctc cagttgcctg cagcgtccgt
    21121 cgtcgcggtg taattttgcc cgttaagcgc cagcgtaatc gtggtgcctg ctggctgatt
    21181 actggtgccg gaaatttgca gatccgcgcc tttttcgctg gcgttaatta tgtcatcggt
    21241 ggcgatggtg ttgatgtcaa tggttggtgc ggtgaggtta acggtgaccg tgtgtgtcta
    21301 attgtcgctg ttgcctgccg catcggtaac cgtggcggtg acagtttgcg ggccgctgcc
    21361 aagcgccgtg acatccgccg ccggaacgcc gacgctccag ttgccggagg cgtccagggt
    21421 ggtggtgtag gttttactgt ttagcgtgac ggtgacgaca tcccccgctt ctccgccggt
    21481 gctggaaccg gaaatcacca gcgcctgccc gtgttcggtg gcgttaataa tgtcatcgcc
    21541 cgcaatggtg ttgatggtca gcaccggaac ggtgagatcc accgccagat tatgcgtggt
    21601 gctcgccgga ttgcccgctt tgtcattgac cgtggcggtc agggtgtggc tgccatcggt
    21661 caatgcctca agatctgccg ccggaatatt gatgctccag ctgccgtccg cctgcaccgt
    21721 tgcctgatag gtctgcacgg tcgggctgtt aagcgttatc gtaacaatct gccccgcttc
    21781 ggcggtggta ctgccgctca ccgtcacgcc cgcatcggct tcgctggcgt tgacgatatt
    21841 gtcgctggcg acggtattga taatgatggt tggcgcgctg ctgtcgacgc tgtagttgtg
    21901 caccgccgag gcgctattgc cgttgatatt gctgacgctg gccagtgcgg aggcgctgcc
    21961 ctcgggtaat gacgccagat cggcggcggg tacggtggca gtccagctac catcgctcgc
    22021 tacgctggca gtgtaatttt tcccgccaaa ggtgacggtg acggtttgac cgggttctac
    22081 gttagtggtg gtgccggaaa gggttaaatc agcgcctttt tctgcggcgt taatcacatc
    22141 gtccgtggcg atggtgttga tggtgatcgc tgccggagtg agatccaccg tcaccggatg
    22201 cgtaatgctc accgagtttc cggcgctact ttcccctgaa acggcaatat taaccgtccc
    22261 cgcaggccag gcgctaacct gcgccgccgt gacgcccacg ctccagctac catcggcctg
    22321 caccgcagtg gtgtattcca cattattaat cgtaacggta agcggcgtgc tttcagccag
    22381 ccccgagctg ctgccggtga ccaccagcgc ctgcccgtgc tcgatgatat tgaccacatc
    22441 atcgcccgcc accgtatcga cccgcaggcc gggcagattg gcgtcgatgg tgatatcccg
    22501 cgtgccgctg ccggtgttgc cgttttgatt ggtgactgag gcattaaccg ttaaatcgcc
    22561 atttcccagc gcctgaatat ctgccgctgg aacgtccacg ctccaggtga gattgctgcc
    22621 caccgtcgcc gtataagtat taccacccaa cgtaatagtt accgtgtcgc catcttccgc
    22681 gccagtcacc tgcccgctga tggtttgcgc cacgcccgct tcggcggcgt taataatatc
    22741 gtcggttgcc accggattaa ttgtcacacc gggcagcgcg ctgtcgacca gcacgttatg
    22801 gctggcagtg gcgctgttgc cgatatcgct ggtcaccgcc gccgttaccg tatagttggc
    22861 ctggcctaat gctgtaaccg ccgatgccgg aacggtgacg ctccagttgc cggaggcgtc
    22921 ggtcgtggtg gtgtaatttt gcccgttcag cgtcacggtg atggcggtgt taacaggctg
    22981 atcgctggtg ccggtaatct ggagatcggc ccctttttcg ctggcgttaa tcacatcatc
    23041 gcccgcaatg ctgttaatgc cgataagggg cgcggcggta ttgaccgtga cggtcaatga
    23101 ctggctgccc gtgtttccgg ctttgtcggt taccgagacg ctgacaggat aactgccgtc
    23161 cgccagccca ctgaccaccg aggccggaac gcccagactc cagttgccgg aaccatccac
    23221 caccgtggtg taatccacat tattcagcgt cacggtgacg atatcgcccg cctgagcgcc
    23281 agtaaccgtg ccgctgatga tttgcgcctg agcatgttcc ggcgtgttga tgacgttatc
    23341 ggtcgccacc ggattaatgg ttaccaccgg cggcgtggca tccaccagcg ccacgcgtga
    23401 aacgctgctc ggattaccgg cgacatcgtt cacgctggcg gtgaccgtag cgttgccgtc
    23461 agccagtgcg cccacgttgg cggcgggcac agtcacgctc caggtgccgt ctggctgcac
    23521 gacgccctga tacgtctgcc catttaacgt aacggtcacc gtctgccccg gctgagcgtc
    23581 ggtggtgccg gtgatgggca gatcctgccc gctttcggcg gcgttaatga tgttatcagc
    23641 ggtcagtgga tcaatgctta aggccggggc ctggctatcg acggtaatgg tgcgggaagt
    23701 gttgccggta ttgccgctga gatccgtcac agaagcggta atcgccaccg cgccgtcagg
    23761 cagatttccc atcgcggcgg caggtaccgt cagactccag gagccatcgg cctgcacggt
    23821 agtggtaaaa ctctgtccag ccagggttac tgtgacggtt tgtcctgtct cagcctgttg
    23881 agtgctaccg gtaagggtca gtggcgcacc tttttctgcg ccactgataa tgtcatcgcc
    23941 ggagacggtg ttaatggtga tcaacggaga aacaccgacc agcgtgattg ctcgagacgc
    24001 cgcgccagta tttcctgcag catctgtcac ggtgacgaaa atcgtattgg ctccgcgatc
    24061 cagcgtgcgg gttaccgctg ggtccagcgc cacattccag ctgccatctg ccagcacgat
    24121 gcccgtcagg acatgagtgc ccaatttcac cgttaccgta tcgcctggag aagcaccgct
    24181 gacttgtccg gagatgatct gcgcgacggc ctgctcggca ttattaagaa tatcgtcgcc
    24241 cgccacggta ttaacactga ccacgggtgc tgaggtgtct accgtgaaat tcgcgctgtg
    24301 gctggtggtg ttccctgccc gatcgctgac actggcattg acagcgtaat taccctccgc
    24361 cagtgcctgg acttccgtgg caggcagcgt cacttgccag gttccgtcgc taccgacggt
    24421 tgctgtatgg cttttcccgt tgacggtcag tgttacggtt tgccccgctt ctgcattcga
    24481 cgtgccgctc agtaccagcg cgacgttatg ttccgcagca ctgataatgt tgtcctgagc
    24541 gatggtgttg atggcaagcg ttggcggttg ggtatcgacc gttagtagct gcgcgccggt
    24601 gaccgtattg cctgtggcgt ctttcccact gaccagcacc gcctgattcc cttcgcctaa
    24661 cgcctgtgca tcggcggtcg gcacctgcac gctccaggca ccgttagcgc ctacgctggt
    24721 cgtgtaggtt ttgccgttta gcgtaacggt gagttctgtt ccctgcgcca gatggctact
    24781 ggtgccagaa agcgtaaacc cggcggcctg ttcgctggcg ttaataatgt tatcgcctgc
    24841 ggtggtggca acctgcacgc tggcctgact attgtcgatg gttaacgtta tcgaaccggc
    24901 gtcagtgatg gtactgccga caatttgcac cactgaccag gtatgttcac ctgcactttg
    24961 cgtgggtaac gagacactcc agtgaccgtt gttgtctacc tgagtactgg caatagtatt
    25021 gccgttactg tctttaatct gaatagtcgc ccccgcctgc ccgctgccgc taaaggttgg
    25081 cgtggtgtca tcggtgatgt cattggtggc tagaatgccg cgctgatcgc cctgattatc
    25141 ggtaactaaa aatgatggtg tggcgttgcc aggctcagca ggtggcgtag ggttattaat
    25201 cacttctgtt ttcgagtcgc catcgccacc gcttgccagc agtgcaccaa gcgcgccacc
    25261 acctaccgcc gcccccgcca gccaacccca cgggaaggcg ctggtctgag caacggtatc
    25321 aagaaatggc gcaatgcttt caatcgcagt ggtctgggca gtcagttcta cgggggctaa
    25381 tccacccgca gcagtatcgg caaaggtgat atgggttagc tgttgaccat cggcaaacac
    25441 cagttccgat tgttcagctg tattcgccgc ttgcaggaaa taaccgttgc agcggatcac
    25501 cgtgccgtcc tgcatataga tcagcaggtc atttccttca cggacgtaac gcgcaacggc
    25561 agaggcctga gcatgaacct gcaccacgga aggctcataa ataacaaccg tgacgtcgcc
    25621 aggattaatc agcgtttgtt tgacggatgt tttacgcgaa ataacatcaa taatcaggct
    25681 cataatggca ttcctgataa atggcgcaca tccttagcga gtccatttat ctaaataaga
    25741 caaaggtaat cacttaacaa cggcccacta ttaataatgg caggattatt tcaagtcggg
    25801 taagtcgttg tataagccct gtttaattcc cgctaatgga ataaggttat tcaccgcagc
    25861 ggcataattt acggcggcga cccagccatc ataattggca tttatttcag ccgattgcgc
    25921 ctgaaagaca tcttgttcga cggttaatag atcattcaga ctgcgtttac ccaatttata
    25981 ttcattttgg tacacatctc gcgttttgtg cgcactttcg gattgcgcta aaccggcttc
    26041 ttcacgaccc cgtgcgccgg tccagttcgc atatgccaca gacgctcgtt gcagcacatc
    26101 cagttttgcc tgttcgacct gcgaggcaga gattttttgt tgcccttcgg cctgttgcac
    26161 ctgggcagaa accgcgccgc cctgatacag cggcgcgtta acgttcagtt gtagctgatc
    26221 atcccaatac gagcggtcgc tggtctggta gcgcgtttta cccccctgaa tactgagcgt
    26281 tggccagtat tgcgctttcg ttttttccac gccgtactgt gctgactggc gtaagttttc
    26341 tgccgccagc accagcggga tggactggta atcaatattc ttcagcgata ccggctgctc
    26401 ggctaattca gcaggtggcg cggctatcgc ctccggctgt acgccagtga gcaccgccag
    26461 ttgcgctttg gcgctcgcca tctgcgcctg atattgctcc agcgttgagc gcattccggc
    26521 aatacgcgtc tgggcctgta attcatccga cgatgagttc aggcctgcgt tagcgcgcaa
    26581 tgccgccatg ttgtagacgt tttccagcga gtgaatattg cgttgagccg catcgcacaa
    26641 agcctgataa cgactgactt ccatgtaggc agtcgccgtt ttctctgcaa catcggttaa
    26701 ggttgccatc aatttaaagc ggtagctgtc acgggcggca gtttgcagat tgatatcgtt
    26761 gttagtttta ccaaagtcat acaccagttg tgtcagggtt atgccatacg aaacgttgtt
    26821 atcaagcctg ccgctggagt ccgtttgtcg ggacggcccc gcgttgccgg ttaatccaac
    26881 gtgcggatac caggcacttt tggcttcatc aatttgcgct tcgccaatac ctatctgcgc
    26941 cgcctgttga gtgacaccgg gatttcgcgc aaaggcgcgt aatatacttt cctgtaaaga
    27001 taattgggca actggcgtta ccgctggggc attcgcccat tgtggcagcg gtgcagcaat
    27061 tgcagcttct cccatcacca taaaacttat aaaaaaaata agtccattat ttttacggtt
    27121 tttaaactgg aatcgcgcca tagataagtc acttccttcc aggtcattat tttaactggt
    27181 ttttataacc agtctcgttt tattaagaag ggtaataact tgtgcatctc ttttgtcgat
    27241 cattgatatg ggcaaagaaa tgcagctaaa aagcttatac gttatttatt tagaatacac
    27301 tacgactttt agattaccat catacacatt aaaaataatc cacggtaata agtgattagc
    27361 atcacagatt tatcgcgtat atatcatttt tttgtgactt atttattaaa ggaataagaa
    27421 aggtaatttg aattaaagac aaaggcgcag tacaggcagt actgcgcgca gagaggtgtt
    27481 atacggtttt attgcgcttc atgaccattg ccacaataag gctgagtaag attgcaccaa
    27541 taatccatat caacgcacgg ctgccctggg accacaggct gtagataaag ccaacgatca
    27601 ccaacaacat caccaacgta cctacgataa gaatcggcaa cgaggcgtga atatcatgac
    27661 gcaggcggat cgccaccaca aacaccgcca gatagcaaat caaaaaggtg gcgctggcaa
    27721 cgctggcgag tgagcctaaa tttagtgccg ccgtcatcag cataatcaac accacgacaa
    27781 tgatgttgcc ccaggtactc tgccgccaca gggatttatt cattcgcttc ggcagttcgc
    27841 gttcgctgcc catgttgtcc atgatgttaa acacggcgaa caggttcgcg ttgatggctg
    27901 aagccgtcgc cagtaaagcg ccgatgacga cgatcacata acccacatgc ccgagcagcg
    27961 gagaagcagc ctgcgctacg gcggtatcgg catatttttc taactctaat gccgatacat
    28021 cgctaagcaa aaccagcgcc agcgagatat aaagcaacgt ggtaacgcca attgccacca
    28081 gaaacgcccg tggcataatg acctgcggat ctttcacttt atccgccgcg ttcgccatca
    28141 tgccaaagcc cgcataggca aggaaggtta tcccgataca ggagaagaac gcaccggagc
    28201 tggggggcgc agagacggaa atatgcgccg gttgcagcga ccagacaccg gcaataatca
    28261 gcaataacag gatcatcatt ttaatgccga cgaggatcac ctccagccgc cctaccgcat
    28321 ggttgcttaa ggagttgaaa agcgtcatca ccgcaatgat ccccaacgcg tagagcaaaa
    28381 taaggtgctc ctcctggctg ccttcatgca aaaactgcac ggcataagcg ccaaaagcac
    28441 gggcgaccat ggcgatgctc accgccagcg tcaacaggta cagtaacgag agcgccagcg
    28501 aaaagacgcc gttgcctaat ccgcgacgaa agaagtcgat aatgccgcca ttgctggcat
    28561 agctcgcccc cagacgcgca taggcataac cggaaaacat cgccacaata ccgccaaaag
    28621 caaaggcgac ccaggtcgag gcttccatta gcaacgcagc ctgccccagc agcgcgaaga
    28681 tccccgcccc caccattgcc ccaatgccga tggaaacgac gttccataga ccgaggggtt
    28741 tgttaccgtt attaccttcc gtgttcatca tgatcagccc ttaaacacgt tatagccgag
    28801 ttgcttagcg accgatgcca ccattttttg accgcgaaca ctgttgcctt cttcgtccag
    28861 cggtggtgag aacgcggcaa ttcccatcac tccagggacg accgccagaa taccgccacc
    28921 tacaccgctt ttgcccggta aaccaacacg atacgcccag tcaccggagc gaccatacag
    28981 cccttccatc atcatttcgg ccagaatgta cggcacgttg tcggcctgaa gaacgcgttt
    29041 atgcgtcaac ggattcacac cacccgccgc cagcgtcgcg ccaagcgttg ccagttcaac
    29101 agtattgatg agcgtggagc actgacgggt gtacacgtca caggcttcca ttgcatcaca
    29161 atagagatat ccggcggagt acagcagcca ggcaatggcc cggttatgga agttggttgt
    29221 ttgttccgac tggttgactt cgtcagagag cgctaccagc tcaccagcca gttgctgttg
    29281 gatatgtaaa attcgctgcc agcgttgttc ggcattttca gcgttaatca ggctggtggt
    29341 ggcaatagcg ccagcattta ccagtggcga cagcggtttg ccgccatgca actctaaggc
    29401 gataactgag ttaaagggca atccggtcgg gtcagcgcca attttgtcct gtaccgcctg
    29461 cgggccgaca tcttctaacg caagggctaa cgtacagact ttcgagatgg attccagcgc
    29521 aaagcggtaa tcactgtcac ccgcactata gacgttgcca tcgctggtca cgatagccac
    29581 tgctgccagt tgaccaggca cattcgccag aaagggaatg taatcggcat tttgtccgcc
    29641 gttaagtgag tgaaattggg tgtaagcctg atccactgcc tgctgtaatt tgtttgcatc
    29701 taacatcttt tgttaactcc tttttataga tgcgggaggt aattcctcac cccggtgccg
    29761 attttcaggc atcctgattt aacttagcac ccgcaactta actacaggaa aacaaagaga
    29821 taaatgtcta atcctgatgc aaatcgagcc gattttttaa tctttacgga cttttactcg
    29881 cctgggttat taatttcttg accttcccct tgctggaagg tttaaccttt atcacagcca
    29941 gtcaaaactg tcttaaagga gtgttttatg tcacaaacta tcgacctgac cctggacggc
    30001 ctgtcctgcg gtcactgcgt taaacgcgtg aaagaaagtc tcgaacagcg cccggatgtg
    30061 gaacaagcgg atgtgtctat cactgaagcg cacgttaccg ggactgccag tgcagaacag
    30121 ctgatcgaaa ccatcaaaca agcgggttat gacgcatctg taagccaccc aaaggctaaa
    30181 ccgctggcgg agtcatcaat cccgtcggaa gcactgacag cggtttctga ggcgcttccg
    30241 gcagcgaccg ccgatgacga tgacagccag cagttgctgc tgagcggcat gagctgcgcc
    30301 agctgtgtca cccgcgtaca aaatgcgctg caaagcgtac cgggcgtcac tcaggcacgg
    30361 gtaaacctgg cggagcgtac tgcgctggtg atgggcagtg cctccccaca agatttagtg
    30421 caggcggtgg aaaaagcggg ctacggcgcg gaagcgattg aagatgacgc gaaacgccgc
    30481 gagcgccagc aagaaaccgc cgtcgctacg atgaagcgct tccgctggca ggcaattgtt
    30541 gcactggctg tgggtatccc ggtaatggtc tgggggatga tcggcgataa catgatggtc
    30601 accgctgaca accgcagcct gtggttggtt atcggcctga taaccctggc agtgatggtt
    30661 ttcgccggcg gccattttta ccgcagtgca tggaaaagcc tgctgaacgg tgcggcgacg
    30721 atggatacgc tggtggcgct gggtacgggt gtggcgtggc tctattcgat gagcgtcaac
    30781 ctgtggccgc agtggttccc gatggaagcg cggcatcttt actatgaagc cagtgcgatg
    30841 attatcggtc tgattaatct cggtcatatg ctggaagcgc gcgcacgtca gcgttcttca
    30901 aaggcgctgg agaaattact cgatttaact ccgccgacgg cgcgcctggt tactgacgaa
    30961 ggtgaaaaaa gcgtgccgct ggcggaagtt cagccaggca tgctactgcg cctgacgacg
    31021 ggcgaccgcg tgccagtaga tggcgagatt acccagggcg aagcatggct ggatgaagcg
    31081 atgctgacgg gcgaacctat cccgcagcaa aaaggcgaag gcgatagcgt ccatgccggg
    31141 acagtggtac aggacggtag cgttctgttt cgcgccagtg cggttggcag ccataccacg
    31201 ctatcaagga ttattcgtat ggtgcgccag gcccagagca gcaagccaga aatcggtcag
    31261 ctggcggata aaatctcagc cgtatttgtg ccggtagtag tggttattgc gcttgtcagt
    31321 gcggcaatct ggtatttctt tggtccggca ccgcagattg tctataccct ggtgattgcc
    31381 accacggtgc tgattattgc ctgtccgtgt gcgctggggc tggcgacgcc gatgtcgatt
    31441 atttccggcg tcgggcgggc ggctgagttt ggcgtgctgg tgcgggacgc cgacgcgctg
    31501 caacgcgcta gtacactcga taccgtagtg ttcgataaaa ccgggacgct gaccgaaggg
    31561 aagccgcagg ttgtcgcagt gaaaacattt gctgattttg acgaagcgca ggcgctgcgt
    31621 ctggcggcgg cactggaaca aggttccagc catccgctgg cgcgggcgat cctcgataaa
    31681 gcaagcgata tgcagctgcc gcaggtcaac ggtttccgca cattgcgcgg gctgggcgtg
    31741 agcggtgaag ctgaaggtca tgcgttattg ctgggcaatc aggcgctgtt aaatgaccag
    31801 caagttgata ctaaagcgat tgaagcagat atttcagctc aggcatcgca aggggcaacg
    31861 cctgtgctgc tggcggttga cgggaaagcg gtagccctgc tggctgtacg cgatccgttg
    31921 cgtagtgata gcgtggcggc gctgcaacgt ctgcataaag cgggatatcg tctggtgatg
    31981 ttgaccgggg ataacccaac caccgccaat gcgatcgcca aagaagcagg gattgatgag
    32041 gtgatcgccg gggtgctgcc ggacggtaaa gccgaagcga ttaaacgcct gcaaagtgaa
    32101 ggacgtcagg tggcaatggt gggcgacggc attaacgatg cgccagcgct ggcccaggcg
    32161 gatgtcggca ttgcaatggg cggcggcagt gatgttgcca ttgaaaccgc ggcgattacc
    32221 ctgatgcgcc atagcctgat gggcgttgcg gatgcgctcg ctatttcccg cgcaacgctg
    32281 cgcaacatga agcagaacct gctcggcgcg tttatctaca acagtatcgg tattccggtc
    32341 gccgccggta ttttgtggcc gtttactgga acactgctta acccggtagt tgccggagcg
    32401 gcaatggcgc tctcgtcgat taccgtagtg agtaacgcca accggttgct gcggtttaaa
    32461 ccgaaggaat aagtacgtca ttgcggatgc gctgctttag ggtggcgcat ttggcatcga
    32521 cgcatgactg ccggatgcgg cgtaaacgcc ttatccggcc tacggttcag gcacaggttt
    32581 gtaggcctga taagacgcgt aagcgtcgca tcaggcaatg gtgctcggat gccggatgcg
    32641 gcgtgaacgc cttatccggc ctacgagatg aagcgatgtt ttggccttac gcgacctttt
    32701 ttgcacgctc ttcacggcgt gccaaatatt cagccactgt ttcaatccgt cccaattctt
    32761 cctgggtgcg catgttgttt tcaacttccc aaagatcaac tgccgcctgg aggtttagcc
    32821 aaaaatcgac tgtggtatca aaaactttcg ccagacggaa tgccatctca gtggtgagtt
    32881 tacgattgtt attgatcagt gcactgacgc tattacgatg aacatgcagc aactctgcta
    32941 actcattgat tttcaaatct agcggttcca gatattcata gagaagaata tctccgggtg
    33001 tcgtcggttt tcttgttgcc tgtttcatag tttttaactg ttgcttccct gtaaatagcg
    33061 atacaagaac atactgaatc atacaaacac attaaacaag tccccaactc catttgcgcc
    33121 atccctcccc gcgcgctagc atgatatttc acaaagggag gtcgtatgga tctgttgtac
    33181 cgggtaaaaa cgctttgggc cgcgctgcgc ggtaatcatt acacctggcc tgccattgat
    33241 atctctcttc ccggcaatcg ccattttcat ctgattggca gtattcatat gggtagccac
    33301 gatatggctc ccctacccac ccgtttgctc aaaaagctca aaaacgccga tgcgctgatc
    33361 gtcgaggcgg atgtttccac cagcgatacg ccttttgcta atttgcctgc ctgcgaggcg
    33421 ctggaagagc gcattagcga ggagcaactc caaaaactgc agcacattag ccaggagatg
    33481 ggcatttctc cctcactctt ttctacccaa ccgctgtggc aaatcgcgat ggttcttcag
    33541 gcgacgcagg cacaaaaact ggggctgcgg gcagaatacg gtatcgatta tcagctattg
    33601 caggcggcga agcaacaaca taaacccgtg attgaactgg aaggggcgga aaaccagatt
    33661 gccatgttgc tccagctccc tgacaaagga ctggcgctgc tggacgatac gctgacccac
    33721 tggcatacca acgcacggtt gctgcaacaa atgatgagct ggtggctgaa tgcgccgccg
    33781 caaaataatg aaataacgct gcccaatacg ttcagtcagt cgctgtacga tgtgctgatg
    33841 catcagcgaa atctcgcctg gcgggataaa ttacgcgcca tgccgccggg gcgatatgtg
    33901 gtcgcggtcg gtgcactaca cctgtatgga gaagggaatt taccgcaaat gttgcgctaa
    33961 aaaaatggcc aatatcgcta ttggcccgtc aaagaggaat ttcatttttt tattattatg
    34021 ccgtcacttt aagcgacggt gtaactcgat tgtcgctcaa tctcaccatc ctgccaatac
    34081 tcttaggcaa gatgactctt ttttttggac cgccatcagg cgtatgcttg cttcgttttt
    34141 ttgtttaagg caggaaaatt accatgacac ccgcagttaa atcactcgaa aaaaacaaga
    34201 tttcgtttca aatccatacc tacgagcacg atccggctga aaccaatttt ggcgatgaag
    34261 tcgtcaaaaa attaggtttg aatccggatc aggtctacaa aacgctgctg gtcgcagtga
    34321 acggtgatat gaaacacctt gccgtggccg ttacgccggt cgcaggtcaa ctggacctta
    34381 aaaaagtagc aaaagcgctg ggtgccaaga aagttgagat ggccgatccg atggtcgcgc
    34441 agcgttcgac gggatacctg gttgggggga ttagcccact ggggcagaaa aaacgtctgc
    34501 caacgattat cgacgccccc gcacaagaat ttgccactat ttatgtttcc ggcggcaagc
    34561 gcggactgga tatcgaactg gcggcaggcg atctggcgaa gatcctcgat gccaaatttg
    34621 ctgatatcgc ccgccgcgat taagcattgt gccggatgcg aacatccggc actttcggat
    34681 tactgccagc tcacctcacc tttcggttca taaacactca catccagcgg cgagcttttc
    34741 tggatatacg ctttcagcac ttcggcatca ataaagccgg tattcacata gcccggtttg
    34801 ttatcaaggc gcggatatcc atcaccgccg gtggcattga agtttaatgt cgccatacgg
    34861 taagttttcg ctggatcgac cggttcgcct ttgattttaa ggtcgttcag tttgccgtct
    34921 ttcgccacaa agctaacgtt ggcaaattgc gggtaggcac ctgaatctgg cttcatctgc
    34981 gcgacggcgg tcaggtaatc aatcacctct ttaccggtca tgtcggcata caccaccaca
    35041 ttgccgaatg gctgcacttt cagcacgttt ttatagctga tatcgcctgc ttcgatagaa
    35101 tcacgaattc cgcctccgct catcaccgca aagtcggcac cagtgcgatc catttgtgct
    35161 gccagaatca accgccccat attggtctgt acaaaacgca ctttgtcacg atcgccttcc
    35221 agacgaccat tggtttcgcc tattttcact tccagctgcg ctttgccttt gttctggaac
    35281 ggcgataaca gcgagatcat ttgctggttt tcagcgattt caggggtgta cagcacgcgc
    35341 tcgcttttcc cgtcttccca ggtcactttc ttcttcaggt tcaccggaat cagctggtag
    35401 ttaaccattt tcatttcgcc gttgcgaaac tcaaagtcgg cccgaccaac gtacttgccc
    35461 cactcatgcg cctgtacaat ccagatgccg ttttgttgat ccggtttgca tggcgtacct
    35521 ggaacgtaat ctacctgctt tttattttcc gctgccatac agactggatc ttgcgagtga
    35581 ccgccgacaa tcatcgccag cgatccggca ggcaacgcgc gtgccatctc cacatcgccc
    35641 ggtgcgttag aaccgtgatc gccgttatcg taatgcccca tatgtgtcgc cgcgataata
    35701 atgtctggct tttctgtctg ttgcagctcc tgaatcacca gcttcgcttc atcggcgggc
    35761 ttacggaact caatatcggt gaagttttct ggattgccta acttcgctgt gtcatccgtc
    35821 gtcagaccaa taacggcaat tttcagatcc tgacgcttaa acagcgccca tggtttaaac
    35881 aggcgctcgc cggtactttt ctggtagata ttggcagaaa gtaacgggaa ctttgcccac
    35941 ttttcctgct ggcgtaatac ggtgagcggg ttatcaaatt catgattacc gatcgccatc
    36001 gcgtcatagc ccaccagatt cataccgcga aaatcaggtt cggcatcctg taagtcagac
    36061 tcgggcacgc cagtgttaat gtcgccaccg gaaagtagca gcacgctacc gccttcagcc
    36121 gcaacctctt tgcggatacc atccaccagc gttttttgcg ccgccagacc atattcgcca
    36181 tattcattgc gccaaaaatg cccatgatga tcattggtat gcagaactgt aattttgtag
    36241 gttttatcct gctcatacgc cagagcagct tcactcgcca gtgtaaatgt ggttaacagt
    36301 gctaacgcca cgccccgctg caataatttc atacttctct ccctgacctg atttcaacgt
    36361 gttgaaaaat agtatgacgc gacatcatag acaagtctgc gtccggtgaa gataaatctg
    36421 catccagatt agaaattctg tattcagcgt tgagacttcc ttcaggcggc gcatacaggt
    36481 atgttagtcg ggtaataatc acaaataaga tcattcttaa catcgcaacc ctgaaaaagt
    36541 gactttccta tggcaatgag tgaacaaacc cagcctgtgg cgggcgcggc tgcgtcaacg
    36601 accaaggccc gaacatcgtt tggtatttta ggtgctatca gcctctcaca tctgctgaac
    36661 gacatgatcc aatcgctgat tctggcgatt tatcctctgc ttcagtcaga attttctctg
    36721 acatttatgc agattggcat gataaccctc accttccagc tcgcctcttc gctactgcaa
    36781 ccagtggtcg gctactggac cgataaatat ccgatgccgt ggtcgttgcc aattggcatg
    36841 tgctttacct taagtggtct ggtgctgctt gcgctggcgg gcagttttgg cgcagttctg
    36901 ctggcggcgg cgctggtcgg taccggttca tcggtctttc atccggaatc ttctcgcgtg
    36961 gcccgtatgg cttccggcgg gcggcatggc ctggcacaat ctatctttca ggtcggcggc
    37021 aactttggca gttccctggg acccttgctg gcggcggtga ttatcgcgcc ttatggcaaa
    37081 ggcaacgttg cctggtttgt gcttgcggca ctgctggcga tcgtggtgtt ggcgcaaatc
    37141 agccgttggt actcggcaca gcaccgaatg aataaaggaa aacccaaagc gacgattatc
    37201 aatccactgc cgcgcaacaa agtggtactg gcggtcagca ttctgttaat cctcattttc
    37261 tcgaaatatt tctatatggc gagcatcagc agctattaca ccttttatct gatgcaaaaa
    37321 ttcggattat ctatccagaa tgctcagctt catctgtttg ccttcctgtt tgccgttgcg
    37381 gcaggtacgg tgatcggcgg gcctgtaggg gataaaattg ggcggaaata tgtgatttgg
    37441 ggctctatcc tcggcgttgc gccgtttacg ctgattttac cctacgccag cctgcactgg
    37501 acgggggttt taacggtgat tattggattt atcctcgctt cggcattctc tgccattctg
    37561 gtctacgctc aggagctgct tccaggacgt atcggtatgg tttctggact ctttttcggt
    37621 tttgctttcg gcatgggagg tctgggagcg gcagttctgg ggcttatcgc cgatcacacc
    37681 agcatcgagt tagtctataa aatatgtgct ttcctgccac tattggggat gttgaccata
    37741 ttcctgcctg ataaccggca taaagactga tttcattgca gccaaaggca aactttggct
    37801 gcatcgtttt acagtcgcca taagcctttc ctctgttaaa ccgccttctg ttttccgatc
    37861 cgtgcttttt tgcagctaat tccatttttc tgcaaaattc aataattatt catgaacatc
    37921 atcaatgaaa ttgtcataaa ctattacctg taattttggt tttcccggcc aaaaatggac
    37981 aataaccacc aggaaaagga gacggaatgc atcacgccac cccgcttatc accaccattg
    38041 ttggcggcct tgtgctcgcc tttatcctcg gcatgctggc caataaacta cgtatttctc
    38101 ctctggtggg atatctgtta gcgggtgtgc tggcaggacc attcactccg ggctttgttg
    38161 ccgataccaa gcttgcgccg gaactggctg aactgggcgt cattctgttg atgtttggcg
    38221 tgggtctgca cttttcgctc aaggatttga tggcggtaaa ggccatcgcc attcccggtg
    38281 cgatcgccca gatagccgtg gcgacgctgc tgggtatggc gctctctgcc gtgctgggct
    38341 ggtcgttaat gaccggtatc gtgttcggtt tatgtctttc caccgccagt accgtggtgt
    38401 tactgcgcgc acttgaagaa cggcaattaa ttgacagtca gcgtgggcaa atcgccatcg
    38461 gttggttgat tgtggaagac ctggtaatgg ttctgacgct ggtgttgctg cccgcagtgg
    38521 caggaatgat ggaacagggc gatgtgggct ttgccactct tgcagtcgat atggggatca
    38581 ccatcggcaa agtgatcgcg tttatcgcca ttatgatgct ggtaggtcgc cgtctggtgc
    38641 cgtggattat ggcacgcagc gcggcaaccg gttctcgcga gctgtttacc ctgtcggtgc
    38701 tggcgctggc gttagggatt gcctttggtg cggtagagct gtttgatgtc tcctttgcac
    38761 tcggtgcgtt ctttgccggg atggtactga acgagtctga actgagtcac cgtgccgccc
    38821 acgatacgct gccattgcgc gacgcgtttg cggtgctgtt ttttgtctcc gtcgggatgt
    38881 tgtttgatcc gttaattctg attcagcaac cgctggcagt gctggcgacg ctggcgatta
    38941 ttctgtttgg taagtcgtta gccgcgtttt tcctggtgcg actgtttggt cactcccaac
    39001 gtacggcatt aactatcgcc gccagcctgg cgcagattgg tgagttcgcg tttatcctgg
    39061 cgggactggg aatggcattg aatttactgc cgcaggccgg acaaaacctg gtactggcag
    39121 gggcgatcct gtcgattatg ctcaacccag tactgttcgc actactggag aaatatctgg
    39181 cgaagaccga aacgctggaa gagcagacgc tggaagaggc aatcgaagaa gagaagcaga
    39241 tcccagtgga tatttgcaac catgcgctat tggtgggtta cggtcgtgta ggcagcctgc
    39301 tgggggagaa attgctcgcc tctgatattc cgctggtggt gattgagacg tcacgaaccc
    39361 gtgttgatga gctgcgagag cgcggggtcc gcgcagtatt gggcaatgcg gcgaacgaag
    39421 aaattatgca actggcgcat ctggaatgtg caaaatggct gatcctgacg atccccaacg
    39481 gttatgaagc gggtgagatt gtggcatctg cccgcgcgaa aaatccggat attgagatta
    39541 ttgcccgcgc ccattatgac gatgaagtgg cgtatatcac cgaacgtggt gcgaatcaag
    39601 tagtgatggg cgagcgtgaa attgcccgta ctatgctgga actgctggaa acgccaccgg
    39661 cgggtgaagt ggtgacgggg taaacggtgg ttgccggatg taaaatatga aaaatatgta
    39721 ggcatgataa gacgcgccag cgtcgcatca ggcattgaga gcaaaatgcc agatgcggcg
    39781 tgaacgcctt atctggccta cgaaccgacg ataacttaac gatcccagta agactcttcc
    39841 aggctgtctt cacgctccgg caagccgcgc gttaaacgag gtgaatgctg gttcagtacc
    39901 tgatagctca cacggttagc atatttacac acctgcgcta acgatgaata agttaaccag
    39961 gtgaatttat gtttgctgga gtttggtacg ttgctacgat ggtagctgtt ggcggtaatg
    40021 tcatgcagca acgctgccag tgcgccatcc cctgctccgt tggtgttcat gattttttcc
    40081 ggcccgccca tgtacggcgc aatgtgcgaa tagacacgca gcggattctg acaatccttg
    40141 tggcgcatgg cgcggctaaa ctcatactgg ttgaattcgg ctatagcgcc cggcagcaac
    40201 ggatgctggg ttttacgttt cgcttcgtct tcggtaaagc ccgccatata caagccgatt
    40261 ggcccggcgg tgcacagcac cagatctacc cagtccagcg ccttgtcaga tgccaacaac
    40321 ggatcgcttt ctccggtcaa cgcttcggct tcatcttcgt tcatcgcaag gatagagacg
    40381 tggtctttga ggaattgctg ccaccactgt ggattctcgg caatgacaaa cttagttccc
    40441 agcgtcagca ccaccggtac gttatatttc ttcgcgtact caatggcttt catggttgct
    40501 tccggcatgg gttcacccgg cttgcaacgc accagataag aggtgagaac cagagccgag
    40561 gctccggcaa tcacatcttc cggaatactt tcagcccgca gctggttcat gtggccaggg
    40621 ctgatagcaa aggtacgttc cccggactcg ccaatcagcg taaagcaacg accaatcggg
    40681 ccatccacgc cttgtagata gttaagatcg gtacggctgg aggtgttaca caggtaacga
    40741 taggcatagc tgccaatttc aatattgctg cacatgacgc ccagcagcac cgaacggtcg
    40801 tccgcgagca ccgagtagtt atgcatggtg ttaccaatgg tgccacccgc aaactgatgg
    40861 gtaatcaggt ttttctgttt tagttcctga taaagcgctt cggctacatc atcctcaatc
    40921 accagtgaat gcccggcgct taatccataa cgctcaatga attcatcatc cactttcgct
    40981 tcaatatcga ccagcgtttg atcgataccc actacccagg cggcgctggt ttcgttttct
    41041 ggctggaatt gctgaagcag cggatcgcgt gcatttacgg ggaagtaatg tttggattta
    41101 cgtttaccgg gaaatttcat ggttgttttt acgggtggtt aactgagcgg cgaatgttaa
    41161 cacaatctga cgggagcgcg cgagacgcag ccagtttcaa tatcagcagg cgggatcgca
    41221 ttttcatggc agtggttcct tacaatgacg taatttgaaa ggagtttttg tatgaagccg
    41281 gaaaacaaat tacctgttct ggaccttatt tctgctgaaa tgaagaccgt tgtgaatact
    41341 cttcagccgg atttaccgcc ctggcccgca acgggaacga ttgctgagca acgacagtat
    41401 tacacgcttg agcgccgatt ctggaatgcg ggcgctccag aaatggcaac caaagcttac
    41461 atggttccaa caaaatatgg gcaggtggaa acacgtctct tttgtccgca gccagatagc
    41521 ccggcaacgc tattttattt gcatggaggc ggttttattc tcggcaatct cgatacccac
    41581 gatcgcatca tgcgcctgct ggcaagctac agccaatgta cggtgattgg tattgattac
    41641 accctttcac ctgaagcgcg ttttccgcaa gtgatagagg aaattgtggc tgcttgttgt
    41701 tatttccacc agcaggcgga ggattatcaa atcaatatgt cccgcattgg ctttgccggt
    41761 gattccgcag gtgccatgct ggcgctcgcc agtgcgttgt ggttgcgtga taaacagatc
    41821 gattgcggta aagttgcggg cgttttgctg tggtatgggc tttacggatt acgggattcc
    41881 gtgactcgtc gtctgttggg cggtgtctgg gatggcttaa cgcaacagga tttgcagatg
    41941 tatgaagagg cgtatttaag caacgacgcg gaccgcgagt cgccgtatta ctgtctgttt
    42001 aataatgatc tcactcgcga agttccgccc tgttttattg ccggggcgga gttcgatccg
    42061 ctgctggatg acagccgtct gctttaccag acgttagcgg cgcatcagca gccctgtgag
    42121 ttcaaactct acccaggcac gctgcacgcc tttttgcatt attcacggat gatgaaaacc
    42181 gccgacgagg ctcttcgcga cggcgctcag ttctttaccg ctcagcttta gcgatacgcg
    42241 gcaacaagat tagccatcat ttcgatatgt tccggtgtgg cattaagcgc cggaatatat
    42301 tcatattttt tcccgccggc accgaggaag acctcacggt tttgctcggc aatctcttcc
    42361 agcgtctcca gacaatccgc agcaaagccc gggcacatca cctgaatatg acctacgcct
    42421 ttttctccga gcattttcag cgtttcgtcg gtataaggca tcagccaggg ttcccgacca
    42481 aagcgcgact gaaaggtcat catcactttt tccggtgcca tccctaatgc ggaagccagt
    42541 tcacgagtcg ttgtgcggca acgttgcggg taatcatcgc cttcatctgc ataacgctgg
    42601 ggaatgccat gataagagag cagcagcaga tccggttcgc catgtttggc aaaagaagcg
    42661 cgtacgctgt tcgccagtgc attaatgtaa tcgtggttat cggcgtaatc acgtataaac
    42721 gatatccccg gaatgctacg tttgcgcgcc agaatgcgtg ccagttcatc ccataccgca
    42781 ccgaccgtag aacaggagaa ttgcggataa agcggcagca ccacaatatg atctacatgc
    42841 tctgccagga gttcatctac ggcgctttcc agtgatggcg agccgtagct cattcccagc
    42901 gctacgggcg tctccggtaa acgttgtgcc agcgcctgct gttgctggcg gctgtaaacc
    42961 atcagcggcg agccaccttc catccagaca gaggcataca gcttcgccac acgcggcgag
    43021 cgcagcggca aaatcacgcc gcgcagcaat ggccaccata acaaccgtga ggtatcaacc
    43081 acgcgtctgt cgcttaaaaa ttgtttcaga tagcgtttta ccgcttcagg tgtgggggca
    43141 tcgggcgtac ccaggtttgc cagcaggata ccggttttag tctgacgcat taccgcctct
    43201 tatcgattca acttgttgat aattgtagcg gaaaagtgag gaagaagaac taattgctgt
    43261 gagaggtatt gcctgaggtg gatgagcaga cctgcccgaa agcagatctg ctgcggcttg
    43321 attagccgag gattttttcc agatcagcgc gaacttcagc aaccggcttg gtgccgtcaa
    43381 ctttcgcgta tttggtgtta cccgcttccg cttctttgga gtagtagccg atcagcggtg
    43441 ctgtcatctg atggtattca accagacgtt tacgtacggt ttcttcctga tcgtctttac
    43501 gggtagtcag ttcttcaccg gtaacgtcgt ctttaccttc tactttcggc ggattgaatt
    43561 taacgtgata aacacgacca gacggcgcgt gaacgcggcg accgacgata cggtcaacga
    43621 tcagttcgtc cggtacgtcg aattccagaa cgtaatcaac attgatgccc gcttctttca
    43681 tcgcgtctgc ctgcggaatg gtacgcggga agccgtccaa caggaaacca ttacggcagt
    43741 cttcctgagc aatgcgctct ttaaccagcg cgatcaccag ttcgtcggtg accagtttgc
    43801 cagcatccat aatgtctttt gcttgtttac ccagctcgga gccagatttg accgcagcac
    43861 gcagcatatc gccagtggag atttgcggaa taccatattt ctccatgatg aactgagcct
    43921 gagtcccttt ccccgcgccc ggagcgccaa gcagaatgat acgcattgcg aaaatcccct
    43981 taaaatgtgt cgaatttttt gaaaaagcga taaacgatac caccatcagg cgattgcctc
    44041 aagaaaggca ccaggggctg aaacggttaa tggcgagata atttgcggaa agtgcagata
    44101 atgaaaagaa aaatgccgga tgacacgaag gtcatccggc attacatcag gaaaccagca
    44161 gctggttcat acgacgaata aacaggttcg gatcttccag cgtgccgcgt tctgccagca
    44221 gtgcctgatc cagcagcagt tctacccact cgctgaactt cgcttcatct tcagtatctg
    44281 ccgcacgttt caccagtacg tgatccgggt tcagttcgaa gatgtatttc acttctggca
    44341 ctttctggcc cgccgcagcg aacagtttcg ccatctgagt gctcatttcg tccgcgtcgg
    44401 tcgaaacgat cgctggcgta tcggtcagac ggtgagtcag acggacatct ttcacgcgct
    44461 cgccgagcag ggctttcaca cggtcgatga acggagtcag tgctttctcc gcttctttcg
    44521 cgctctcatc aacttcgtca gccagttttt ccagcgactc gtcaacttta gacaccgact
    44581 ggaacggttt accgtcgaac tcagtcagat agttcatcat ccactcatcg atgcggtcgg
    44641 aaagcagcag aacttcgatg cctttcttac gcagcagttc caggtgcggg ctgctcttcg
    44701 ctgccgcata gctgtctgca gtgatgtagt agattttctc ctgcccttct ttcatgcggg
    44761 aaacgtagtc ttccagagac acggtctgcg cagaagaatc ggtatgggta gaagcaaaac
    44821 gcagcaattt ggcgatcgct tcctggttag cgaaatcttc cgccggacct tctttcagta
    44881 ccaggccaaa ctgttgccag aaggtctggt atttttccgc gtcgtctttc gccagttttt
    44941 ccagcatttg cagcacacgc ttggtcagcg cattgcgcag gttacgcgtt accgtgctgt
    45001 cctggaggat ttcacgggaa acgttcagcg gcagatcgct ggagtcaatc agaccacgca
    45061 cgaagcgcag atagttcggc atgaactgtt ctgcgtcgtc catgatgaac acacgctgaa
    45121 catacagttt caggccgtgt ttatgatcgc ggttccacat atcccacgga gcctgggacg
    45181 gaatgtacag caggctggtg tactcctgct taccttcaac acggttgtgg ctccaggtca
    45241 gcggatcgtt aaaatcgtgg gcgatgtgtt tgtagaactc tttgtactct tcatcggtga
    45301 tttccgactt gttacgagtc cacagcgcct gcgctttgtt aattttctcc caggagataa
    45361 cggtttcgcc gtctttctct tcgcgttttt cgatctctac cggcagcgcg atatggtcgg
    45421 agtatttgct gatgatggaa cgcacgcgcc agtcatcgag gaactcgtct tcgccttcac
    45481 gcagatgcag ggtgatttca gtaccacgat cttctttggt gatgtcggca acggtatatt
    45541 caccttcgcc agccgattcc cagaagacgc cgttttctgg tttttcgcct gccgcgcgag
    45601 tacgcacggt cactttgtcg gccacgataa acgcagagta gaaaccaaca ccaaactgac
    45661 cgatcagctg gctgtctttc gcctggtcag aacccaggga ttcgaggaat gatttggtac
    45721 cggatttagc gatagtcccc agatggtcaa tcacttcgtc gcgggtcatc cccacgccgt
    45781 tatcggagat ggtcagcgtg cgcttgtctt tatcgaaaga gacacgaacg cgcagttcgc
    45841 catcaccttc gtacaggtcc gggttagaga gcgcacggaa acgcaacttg tccgccgcat
    45901 cggaggcgtt agagataagc tcacgcagga agatttcttt attggaatag agagaatgga
    45961 tcatcaggtg cagaagctgt ttcacttctg actgaaaacc acgagtttct tgtcctttca
    46021 tgtaggtcta cctcaataat gccattttaa ggttaaaaac aggatgaggg agagatgggg
    46081 acaagggaga ataatttcaa gcgagagcag gtgatcctgc tctcgtttgc ttaaaaacga
    46141 atcttatgac gcccggcaag ggagtgtgac agcgtggtgc catcgaccat ttccagttcg
    46201 ccgccaaccg gtacgccatg agcgattcgg ctggcttcca cgtcatattg cgcgcaaagc
    46261 tcggcaatgt agttagcggt agcttcacct tcaaccgtag ggttggtggc gaggatcact
    46321 tcagtgattt tttcctctgc cagacgctgt tccagacgat caaggccgat atcatccgga
    46381 ccgatgccgt ccagcggtga cagatgtccc atcaacacaa aataacgacc tgaaaactgc
    46441 cccgtctgct caatggcgta aatgtccgcc ggactctcca ccacgcagat ttgaccgttt
    46501 tcctgacgac gcggattcga acagatgtta cagacttcct gttcggtgaa ggtgcggcaa
    46561 tcggcgcagt ggccgatttc cgacatcgcc cgggtgagcg cctgcgccag acgcatcccg
    46621 ccgctacgat cgcgctgaag cagcgtgaac gccatacgct gcgccgactt cgggccaacg
    46681 cccggcagac agcgcagtgc ttccataagc tgtgttaaca gcgggctggt ttgcatcaga
    46741 acggcatctt aaagccaggc ggcagctgca ttccggagga tacagaagcc attttttctt
    46801 tctgcgtttc ttcaatacga cgtgctgcgt cgttgaatgc tgcagccacc aggtcttcca
    46861 gcatctcttt gtcgtcttcc agcaggctcg ggtcgatctc tacgcgacgg cagttatgtg
    46921 caccgttgat ggtcactttt accagacctg cgccagattc gccggtaact tccagctgcg
    46981 cgatctcttc ctgcattttc tgcatttttt cttgcatctg ctgggcttgc ttcatcaggt
    47041 tacccagacc gcctttacca aacataggtt tctctctcaa tcacgttaag gatgacgaac
    47101 gtaagctgtg cttacgatca aatggggcgg atactgtctt catccagctc cgcatcgaag
    47161 aaccgacgca gggtctgaat attattatcc gcaataatgg actcgcgcgc ctgcgcaagt
    47221 ttttcttcgt atatcgcctg acgccactcc agcggcgtac gcaccgcggg attatcatct
    47281 tcaactatag tcagttcaac cgttgaacct tttaacatgc tcaacgcttc agccagtttt
    47341 tgctgtgcac cgcggttgtt caaatgccgc tgagaggagc gcaaatgcag acatactgcg
    47401 ttgtcgctct cctctttcca ggcatttaac gccacctgtt cgaccagttt tggtagcgaa
    47461 agttgactca cctgagccgc ccacgcgtcg cgctcaatgg cttctgccgc cagcttcgcc
    47521 gccagttccg gcgttttttc atgttccagc gcttttttca gcgccttcgg tgtggcgacc
    47581 acttcttttt gctgcatcac tggagtggtc gccttccagc gatacgcttc ttttttagct
    47641 ggcgcttttt ccagcgccga tggcaccgga cgcgcctgaa cgcgatcggt gaccgaagcc
    47701 agtctttcca gcgcagcgtt attcaccggc cgcgcgcggg tagcggctgc cggttcactc
    47761 ttttttgctt tggttgctcc ctgcacgcgc tgcaactgct ggcgcgccgc cagcacctgg
    47821 ctggtggttt ccgggagcgg tacagtcggt gcctgctgcg gcgctgattg cggttgcggc
    47881 ggcacctggg ttggcgtcat tactgccgtt ggcgcgacgg gtgcaaagga ctggcgtggc
    47941 acttctggct caggcagcgg catacgcgga tggaacgcca gcgcgcgcag cagcgtcatc
    48001 tcaacgccca tgcggcggtc cggcgcatac ggtaattctt tgcgaccaat caacagcgtc
    48061 tgatagtaaa gctgaatatc cgtcggcggt atggtgcgcg ccagttcacg catccgcagc
    48121 tcgatggcgg ccatgtcgtt gccaagtgca gcaggcgaaa gttgtaccat cgcaatacga
    48181 tgcaacaggc cgagcatttc caccagcaac gcttcccact cgataccacg ggcagcggct
    48241 tcattaatca gcgccattac gcgctcgccg ttggcctcga ccatcgcttc aaccagcgac
    48301 agcgcctgat cgtcgtcaag cgtacccagc atcgcactga ccgcctgggt tgaaacctgg
    48361 ccgtcaccgc tggcaatcgc ctggtcggtc agacttaagg catctcgcag gctgccttca
    48421 gcggcgcgcg ccagcaattg cagcgcccgc ggctcgtgag cgatatgttc ttcattgagg
    48481 atgtgctcaa gttgatgacg aatttgctcg acatccagcg ccttgagatg aaattgcaaa
    48541 cagcgcgaca aaatcgtcac cggcaatttc tgtggatcgg tcgtcgccag cagaaactta
    48601 acgtgctccg gcggctcttc aagggttttt aacagtgcgt taaagctgtg gcgcgacagc
    48661 atatgcactt cgtcgatgag ataaactttg aaacgaccac gcgccggagc gtactggacg
    48721 ttatccagca ggtcgcgggt atcttcaact ttggtgcgcg aggcggcgtc gatttcaatc
    48781 agatcgacaa agcgcccctg ctcgatttca cgacagttat cgcacacgcc gcacggcgtc
    48841 gcggtaatgc cggtttcgca gtttagcccc ttcgccagca gtcgggcgat agaggttttt
    48901 ccgacgccac gggtgccgga aaaaagataa gcatgatgaa tacgccctaa cgacaagccg
    48961 ttcgccagtg cggtcagcac atgttcctgg ccgacgacgt cagcaaaggt ttgtgggcgc
    49021 cattttcggg ctaagacctg ataactcatt ggcaggctct gaaacgctgg aaggtggatt
    49081 ctcgaagggg taatgctaac acagccccgt cagaacggcg aggctgaatg cacatcttgt
    49141 gctgtccgta gcgtgggcag cacaagactg gcgataatta atggcccggg aacgggacaa
    49201 ggctgtagct ggtaatgccc tgtttttcga gacgctgttc gccgccgaga tcgaacaggt
    49261 tgataatgaa cgcagcgtca gccacttcac cacccagacg acggatcagt ttaacggtcg
    49321 cttcgatagt gccgccggtt gccagcagat cgtccaccac cagaactttg tcgcccggtt
    49381 tgatggcatc aacgtggatc tccagctgat cggtgccgta ttccagatcg taagtttcac
    49441 tgatggtttc acgcggcagt ttgcccggtt tacggaccgg tacaaagcca acgcccagac
    49501 ccagagctac cggagcgcca aacaagaagc cacgcgcttc ggtgccgaca actttgttaa
    49561 tgcccgcatt tttgtaacgc tcaaccagca agtcgatgct gagagcgtaa gctttcgggt
    49621 cttccagtaa gctggtgaca tcgcggaaaa gaatgccggg ttttgggtag tcctgaatgc
    49681 ttttgatgct atttttgaga tactcaagct gctgtgcagt cgcggtcata agtgtatgcc
    49741 tgcttgttac ggtggtactc acggcgcgtt ttaaacgtat caaaagtaac agttgtttaa
    49801 cctttgaccg cctgcgcctg tgctcgaaaa cgccagaatg tactggctgt acgcaataat
    49861 tgcaactgcg attgttgtgc ttcagtgctt ttcttgcttt tcatcaatca ccggaattcg
    49921 ccacatatag aaaagcaaac aggcgagaat taccagcaac atgatgcgca cccatggcat
    49981 ctggacgaac cacagagaaa tggcaaacgt gagcaaaata agcaaaatcg cccgcggttt
    50041 gacgccgcgc ggcatcgcat gatgtttctg ccagaaacgt agatagctgc caaaccatga
    50101 gcggtacagc aaccaggcgt gaaagcgcgg ggaagaacgg gcaaagcacc aggccgccag
    50161 caggataaac ggcgtcgtcg gtaataccgg taataccacg cccagcgtgc ccagcactac
    50221 cgccagccag ccaatgatga ttaaaatgat tcgttgcata ttgagtgttg tcagcttaca
    50281 gagtggctac tttagcataa caattatcat tttcattgag gtcttatcgt gaaaaccgcc
    50341 ctgctgctgg aaaaactgga aggacagctc gctacgctgc gtcagcgttg tgccccggtg
    50401 tcacagttcg ccacgctaag tgctcgtttc aacaggcatc tttttcagac tcgtgcgaca
    50461 acactacagg cttgtctcga caaggcgggc gataatctgg ctgcgcttcg tcatgcagtt
    50521 gagcaacagc aactgccgca agtcgcctgg ctggcagaac atctggcggc gcaactggag
    50581 gctatagcgc gtgaagccac agcctggtcg ctacgcgagt gggatagcgc accaccccaa
    50641 attgcccgct ggcagcgtaa acgtattcag catcaggatt ttgagcggcg gctacgtgag
    50701 atggttgccg aacgtagagc ccgcctggcg cgggtgaccg atctcgtgga acagcaaacg
    50761 ctgcatcgtg aagtggaagc ctatgaagcg cgcctggcac gctgccgcca tgcgctggaa
    50821 aaaatcgaaa acaggttagc gcgtttaacc cgctagcaat ggagagagta tgtcactgga
    50881 aaatgcccct gacgatgtca aactggctgt cgatttgatt gtgctgctgg aagaaaatca
    50941 gatcccagcc cgcaccgtgc tgcgcgcgct ggatattgta aagcgtgatt atgaaaagaa
    51001 gttacagagc gatgaggctt cacaaagcga gtaaaaaata aagggcggca tcactgtcgc
    51061 cctttattgt ccttacccta ccgctggcgt cggatcgtcg cctttgtagt cgcgttttac
    51121 ttccgtcacc tcatcgccct tctcgttgtg cagatgcact tcaagctggt taaaggcaat
    51181 gttgatgtcg ttttcacggc acagctgatc gatagtacgg ttcagctcat cgacagtacg
    51241 actacggtca cgcagttcac gcacatacag acgcagctca tgatccaacg tgctggcacc
    51301 aaatgccgta aagaagactt ccggcattgg ttcgtgcatc acccttgggt gctcagtcgc
    51361 cgccttcagt aacactttac gcactttttc cagatcggag ccataggcca cgccgagacg
    51421 gatcaccaga cgcgtagtag tgtcagtcaa cgaccagttg atcagacgct cggtaacaaa
    51481 cgctttgttc gggatgatca cttctttgcg atcgaaatcg gtaatcgttg tcgcacgaat
    51541 acggatttta cttaccgtcc ccgagaagct accaatggtt accgtatcgc caatacgcac
    51601 cggacgttcg aacagaatga tcaaaccgga gacgaagtta ccgaaaattt cttgtaaacc
    51661 aaaaccaaga cctacggata atgctgcggc cagccactgg agtttatccc aagagacgcc
    51721 cagcgatccg aacaccgtca tcgcaccaac agcaataatg atgtagttaa ggatggtagt
    51781 aatggcatac gacgcgccct ggcgcatatt cagtcgcgag agcaccagca cttccagcaa
    51841 accaggcagg ttgcgaatca acgcccaggc caccattgag gcgataatcg caaacaacag
    51901 actacccatg gtgacgtttt tcaccaccgc agcaccagct tcagtaccgt tgtaatgcca
    51961 cagcgtaatg ctgtcgagat agctgaacac ggtgatcaaa tcggaccaaa ttgcccagaa
    52021 catgacaccg aacagcgcaa acatcagcaa catggtaata cgcagcgtct gctggttaac
    52081 ctgttccagt gcaatggtgg gttcttccgg cggttcggca ccttctgcgc cctctttcac
    52141 cagattctgc cgacgcgcca gcgcacgacg ccaggcgata cgccgcgccg ctacgcttaa
    52201 gccgcgcaat accgtctggt agagcaagtt ccagatgatc accagataaa cggtttcaat
    52261 ccaacgcccg gagaggcgca gcgtggtgta gaaataaccc gtggcagtca gcaccatcaa
    52321 tgcaatcggg ataatcgaca gcacggtaat ggtgaccagt cgcatggtgt gcgactcttt
    52381 atcacgccag ctttcgcggc acattggcca taccaggaag gcaatcagca gcaggttgaa
    52441 gaaaatcatc gcctgcccca gcacatcatc catcagatgc agcggggaaa gttctgccac
    52501 cacagaccag aaatggatag gcagcaacgc gagactgatg cggacaattt gccgacgcca
    52561 gtggctggtc tgctgttccg gcatgccgaa gtgacgcacg gcaacgccgt tcttctccag
    52621 cactttccag cacaggccaa acaccagcca gaaaatcgcc agttttttgc tgaacgacca
    52681 tagcagttcg ctgatgttga gctgcatggt caacagaatc aggccaaccg cgagaataat
    52741 caggcacacc ggcagtgcac ggatcaggtc gataaggatc gcttttggtg tgttgagctg
    52801 gctgtcgtta cgcagggaac ccacagcgga agccagtttt tgttgatacg ctttcagcca
    52861 ccccagacgc cagtggatca gcccggcaat caacagcagc ggcaaaccag cgaggaaagc
    52921 gataaaaacg gcgggccagg ctttttccca gttaaccgta attttcatcg acttaaattc
    52981 atctttcagg ctttgcggga acgctttgat ccagtcccag tccattggac ggttactgtt
    53041 cacccaaaag atttgctgag tcaggatgga tttcaggttt ttcgacacac tcattaactg
    53101 ctgctggttg atttgcaggt taatggccat catcagctgg ttacccaact gtttgttgag
    53161 ttgatccagc aattcgcgac gcatatcaac cacttgcaat aacgcatcgt gaacttcgct
    53221 gttgacttcg ttggtgtgac cttcttccag tttgttgacg aacgcatcgc tctggaagag
    53281 tgcatcacgc tgctggttaa cttcaaactg ttcgagacgc aaatccgcga tgcggttggt
    53341 catgttttcc agttcatccg ccgagggcag cgtttgttgt tgctggtaaa ggatacgaga
    53401 caacagcagg ctgcccttca ggacggcaat ctgctcttta atattgcgtt ccgattgcag
    53461 cgcccgctcc agccagtttt tgactttaat gttttgctgc atcaactgat taccgttttc
    53521 agtcgcggta atcagacgct gacttaactg ctggttaatt tccagttcct gcttcaccag
    53581 cggattagcc tgaatacgcg cggcttcatc cggggagacg gcttcctgcg ccgttttttc
    53641 ggttaaagtc aggcgcttgc tgtttaccgc ttcttgcaac agctgcaact ggtgctccag
    53701 acgggcgctg ttcgccgtca cgtaatcacg ttgcttttgc aaggtatcct gcaagacggt
    53761 attcccttcc aggcttttac gctgctggtc aatctccgca ttcagcaacg cctgctgagc
    53821 ctgcattaac actttctggc tgggacgtaa ggccgtctcg ccgacatcag tcccatccag
    53881 acgactgcga atttgttgca gctgctgcga agcgttatac atcgcatttt gcacgcgttc
    53941 gggctgcgtc tgtaacgaaa ccagctggct gttataagac gccagatcgt tttgtgcgtt
    54001 ttgcaaatcg tccagcgcct gggcaacgcg agtttccagc tggcgcaacg aaagcgtgct
    54061 cagaattttg cgcgtttctt cgtcgttatc gacatcgcta agtgctgtta acgccgcggt
    54121 cgcctggcgc attttttccg gcgcttcagc gactttttgc cgtagctgaa cggtctcttc
    54181 ttttacgcga tcgattttat cgagggtggc taatgtatct gtcagatcct gctgcaccag
    54241 tttgtcctga gcagaaagat ctttttgttt atttagtgag tcaagttgcg cctgcaggtc
    54301 cgcttttgtc ggcagatcac cattcgatga cgcccgcgca aacgccgtgt tctggcataa
    54361 caagacaaaa acaaaagcaa taaatgctga aaaaacaaaa tgccgtgatc gtttgtaata
    54421 ctggaacata gtcatgatga atgaaggttt ctgaacctga agaacgacct gaaaaagtca
    54481 aaccgcaaga atatcacgac gcagtgaacc agaatagcaa cgacgaaaat gtccaggaaa
    54541 aatcctggag tcagattcag ggttattcgt tagtggcagg attacgaagc gtggggcaca
    54601 ggagatacat ctccagtaag atggcgacgt aatcgcgggc ttctttttta agatcaaaag
    54661 attgcggggc aaagagccag ttttccatca ggccggaaat atagccgcgc ataataattg
    54721 ctgcccgacg cgtcattaaa tccgcaggca acattttcgc ttcaatacaa tgttttaacg
    54781 tttgttctat acggtcataa ctttccaaac agagattacg ttgtgcctgt tgcacaacag
    54841 ccatttctcc gacaaattcg catttgtgga atataatctc catcaataat cgacgccgtt
    54901 cttctgtcac cgtggattca agaacatgaa ttaatatctc tcttaatact gagagtggat
    54961 cgctagggaa ttttgcctga tattcaagct ctagttcacc aatattggat tctgatagtt
    55021 cccagatctc actgaacaaa tccgacttgt ctttaaaatg ccagtagatt gcaccgcgcg
    55081 taacgccagc tgcttttgca atctcaccca gcgaggtgga tgataccccc tgctgtgaga
    55141 aaagacgtag agccacatcg aggatgtgtt ggcgcgtttc ttgcgcttct tgtttggttt
    55201 ttcgtgccat atgttcgtga atttacaggc gttagattta catacatttg tgaatgtatg
    55261 taccatagca cgacgataat ataaacgcag caatgggttt attaactttt gaccattgac
    55321 caatttgaaa tcggacactc gaggtttaca tatgaacaaa aacagagggt ttacgcctct
    55381 ggcggtcgtt ctgatgctct caggcagctt agccctaaca ggatgtgacg acaaacaggc
    55441 ccaacaaggt ggccagcaga tgcccgccgt tggcgtagta acagtcaaaa ctgaacctct
    55501 gcagatcaca accgagcttc cgggtcgcac cagtgcctac cggatcgcag aagttcgtcc
    55561 tcaagttagc gggattatcc tgaagcgtaa tttcaaagaa ggtagcgaca tcgaagcagg
    55621 tgtctctctc tatcagattg atcctgcgac ctatcaggcg acatacgaca gtgcgaaagg
    55681 tgatctggcg aaagcccagg ctgcagccaa tatcgcgcaa ttgacggtga atcgttatca
    55741 gaaactgctc ggtactcagt acatcagtaa gcaagagtac gatcaggctc tggctgatgc
    55801 gcaacaggcg aatgctgcgg taactgcggc gaaagctgcc gttgaaactg cgcggatcaa
    55861 tctggcttac accaaagtca cctctccgat tagcggtcgc attggtaagt cgaacgtgac
    55921 ggaaggcgca ttggtacaga acggtcaggc gactgcgctg gcaaccgtgc agcaacttga
    55981 tccgatctac gttgatgtga cccagtccag caacgacttc ctgcgcctga aacaggaact
    56041 ggcgaatggc acgctgaaac aagagaacgg caaagccaaa gtgtcgctga tcaccagtga
    56101 cggcattaag ttcccgcagg acggtacgct ggaattctct gacgttaccg ttgatcagac
    56161 cactgggtct atcaccctac gcgctatctt cccgaacccg gatcacactc tgctgccggg
    56221 tatgttcgtg cgcgcacgtc tggaagaagg gcttaatcca aacgctattt tagtcccgca
    56281 acagggcgta acccgtacgc cgcgtggcga tgccaccgta ctggtggttg gcgcggatga
    56341 caaagtggaa acccgtccga tcgttgcaag ccaggctatt ggcgataagt ggctggtgac
    56401 agaaggtctg aaagcaggcg atcgcgtagt aataagtggg ctgcagaaag tgcgtcctgg
    56461 tgtccaggta aaagcacaag aagttaccgc tgataataac cagcaagccg caagcggtgc
    56521 tcagcctgaa cagtccaagt cttaacttaa acaggagccg ttaagacatg cctaatttct
    56581 ttatcgatcg cccgattttt gcgtgggtga tcgccattat catcatgttg gcaggggggc
    56641 tggcgatcct caaactgccg gtggcgcaat atcctacgat tgcaccgccg gcagtaacga
    56701 tctccgcctc ctaccccggc gctgatgcga aaacagtgca ggacacggtg acacaggtta
    56761 tcgaacagaa tatgaacggt atcgataacc tgatgtacat gtcctctaac agtgactcca
    56821 cgggtaccgt acagatcacc ctgacctttg agtctggtac tgatgcggat atcgcgcagg
    56881 ttcaggtgca gaacaaactg cagctggcga tgccgttgct gccgcaagaa gttcagcagc
    56941 aaggggtgag cattgagaaa tcatccagca gcttcctgat ggttgtcggc gttatcaaca
    57001 ccgatggcac catgacgcag gaggatatct ccgactacgt ggcggcgaat atgaaagatg
    57061 ccatcagccg tacgtctggc gtgggtgacg ttcagttgtt cggttcacag tacgcgatgc
    57121 gtatctggat gaacccgaat gaactgaaca aattccagct aacgccggtt gatgttatta
    57181 ccgccatcaa agcgcagaac gcccaggttg cggcgggcca gctcggtggt acaccgccgg
    57241 tgaaaggcca acagcttaac gcctctatta ttgctcagac gcgtctgacc tctactgaag
    57301 agttcggcaa aatcctgctg aaagtgaatc aggatggttc ccgcgtactg ctgcgtgatg
    57361 tggcgaagat tgagctgggt ggtgagaact acgacatcat cgcagagttt aacggccaac
    57421 cggcttccgg tctggggatc aagctggcga ccggtgcaaa cgcgctggat accgctgcgg
    57481 caatccgtgc tgaactggcg aagatggaac cgttcttccc gtcgggtctg aaaattgttt
    57541 acccgtacga caccacgccg ttcgtgaaaa tctctattca cgaagtggtt aaaacgctgg
    57601 tcgaagcgat catcctcgtg ttcctggtta tgtatctgtt cctgcagaac ttccgcgcga
    57661 cgttgattcc gaccattgcc gtaccggtgg tattgctcgg gacctttgcc gtccttgccg
    57721 cctttggctt ctcgataaac acgctaacaa tgttcgggat ggtgctcgcc atcggcctgt
    57781 tggtggatga cgctatcgtt gtggtagaaa acgttgagcg tgtgatggcg gaagaaggtt
    57841 tgccgccaaa agaagccacc cgtaagtcga tggggcagat tcagggcgct ctggtcggta
    57901 tcgcgatggt actgtcggcg gtattcgtac cgatggcctt ctttggcggt tctactgggg
    57961 caatttatcg tcagttctct attaccattg tttcagcaat ggcgctgtcg gtactggtgg
    58021 cgttgatcct gactccagct ctttgtgcca ccatgctgaa accgattgcc aaaggcgatc
    58081 acggggaagg taaaaaaggc ttcttcggct ggtttaaccg catgttcgag aagagcacgc
    58141 accactacac cgacagcgta ggcggtattc tgcgcagtac ggggcgttat ctggtgctgt
    58201 atctgatcat cgtggtcggc atggcctatc tgtttgtgcg tctgccaagc tccttcttgc
    58261 cagatgagga ccagggcgta tttatgacca tggttcagct gccagcaggt gcaacgcagg
    58321 aacgtacgca gaaagtgctc aatgaggtaa cgcattacta tctgaccaaa gaaaagaaca
    58381 acgttgagtc ggtgttcgcc gttaacggct tcggctttgc gggacgtggt cagaataccg
    58441 gtattgcgtt cgtttccttg aaggactggg ccgatcgtcc gggtgaagaa aacaaagttg
    58501 aagcgattac catgcgtgca acacgcgctt tctcgcaaat caaagatgcg atggttttcg
    58561 cctttaacct gcccgcaatc gtggaactgg gtaccgcaac cggctttgac tttgagctga
    58621 ttgaccaggc tggccttggt cacgaaaaac tgactcaggc gcgtaaccag ttgcttgcag
    58681 aagcagcgaa gcaccctgat atgttgacca gcgtacgtcc aaacggtctg gaagataccc
    58741 cgcagtttaa gattgatatc gaccaggaaa aagcgcaggc gctgggtgtt tctatcaacg
    58801 acattaacac cactctgggc gctgcatggg gcggcagcta tgtgaacgac tttatcgacc
    58861 gcggtcgtgt gaagaaagtt tacgtcatgt cagaagcgaa ataccgtatg ctgccggatg
    58921 atatcggcga ctggtatgtt cgtgctgctg atggtcagat ggtgccattc tcggcgttct
    58981 cctcttctcg ttgggagtac ggttcgccgc gtctggaacg ttacaacggc ctgccatcca
    59041 tggaaatctt aggccaggcg gcaccgggta aaagtaccgg tgaagcaatg gagctgatgg
    59101 aacaactggc gagcaaactg cctaccggtg ttggctatga ctggacgggg atgtcctatc
    59161 aggaacgtct ctccggcaac caggcacctt cactgtacgc gatttcgttg attgtcgtgt
    59221 tcctgtgtct ggcggcgctg tacgagagct ggtcgattcc gttctccgtt atgctggtcg
    59281 ttccgctggg ggttatcggt gcgttgctgg ctgccacctt ccgtggcctg accaatgacg
    59341 tttacttcca ggtaggcctg ctcacaacca ttgggttgtc ggcgaagaac gcgatactta
    59401 tcgtcgaatt cgccaaagac ttgatggata aagaaggtaa aggtctgatt gaagcgacgc
    59461 ttgatgcggt gcggatgcgt ttacgtccga tcctgatgac ctcgctggcg tttatcctcg
    59521 gcgttatgcc gctggttatc agtactggtg ctggttccgg cgcgcagaac gcagtaggta
    59581 ccggtgtaat gggcgggatg gtgaccgcaa cggtactggc aatcttcttc gttccggtat
    59641 tctttgtggt ggttcgccgc cgctttagcc gcaagaatga agatatcgag cacaaccata
    59701 ctgtcgatca tcattgatac aacgtgtaat cactaaggcc gcgtaagcgg ccttttttat
    59761 gcataaccta cgaacattaa ggagtaattg aaccaccaac tcaggatctc atacgaaaac
    59821 cagtattaac cacgta
//
LOCUS       DAZFTN010000016        54866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFTN010000016 DAZFTN010000000
VERSION     DAZFTN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            255..341
                     /gene="leuL"
                     /locus_tag="ACHKV2_001172"
     CDS             255..341
                     /gene="leuL"
                     /locus_tag="ACHKV2_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO6939096.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            434..2005
                     /gene="leuA"
                     /locus_tag="ACHKV2_001173"
     CDS             434..2005
                     /gene="leuA"
                     /locus_tag="ACHKV2_001173"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO6939097.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            2005..3096
                     /gene="leuB"
                     /locus_tag="ACHKV2_001174"
     CDS             2005..3096
                     /gene="leuB"
                     /locus_tag="ACHKV2_001174"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO6939098.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3099..4499
                     /gene="leuC"
                     /locus_tag="ACHKV2_001175"
     CDS             3099..4499
                     /gene="leuC"
                     /locus_tag="ACHKV2_001175"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO6939099.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4510..5115
                     /gene="leuD"
                     /locus_tag="ACHKV2_001176"
     CDS             4510..5115
                     /gene="leuD"
                     /locus_tag="ACHKV2_001176"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO6939100.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5223..5354)
                     /gene="sgrT"
                     /locus_tag="ACHKV2_001177"
     CDS             complement(5223..5354)
                     /gene="sgrT"
                     /locus_tag="ACHKV2_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO6939101.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5443..7101
                     /gene="sgrR"
                     /locus_tag="ACHKV2_001178"
     CDS             5443..7101
                     /gene="sgrR"
                     /locus_tag="ACHKV2_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO6939102.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7330..7899)
                     /gene="espY2"
                     /locus_tag="ACHKV2_001179"
     CDS             complement(7330..7899)
                     /gene="espY2"
                     /locus_tag="ACHKV2_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO6939103.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8141..8240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8271..9254
                     /gene="thiB"
                     /locus_tag="ACHKV2_001180"
     CDS             8271..9254
                     /gene="thiB"
                     /locus_tag="ACHKV2_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO6939104.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9230..10840
                     /gene="thiP"
                     /locus_tag="ACHKV2_001181"
     CDS             9230..10840
                     /gene="thiP"
                     /locus_tag="ACHKV2_001181"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO6939105.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            10824..11522
                     /gene="thiQ"
                     /locus_tag="ACHKV2_001182"
     CDS             10824..11522
                     /gene="thiQ"
                     /locus_tag="ACHKV2_001182"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO6939106.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11609..12373)
                     /gene="yabI"
                     /locus_tag="ACHKV2_001183"
     CDS             complement(11609..12373)
                     /gene="yabI"
                     /locus_tag="ACHKV2_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6939107.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12459..13337)
                     /gene="araC"
                     /locus_tag="ACHKV2_001184"
     CDS             complement(12459..13337)
                     /gene="araC"
                     /locus_tag="ACHKV2_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO6939108.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13676..15376
                     /gene="araB"
                     /locus_tag="ACHKV2_001185"
     CDS             13676..15376
                     /gene="araB"
                     /locus_tag="ACHKV2_001185"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO6939109.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15387..16889
                     /gene="araA"
                     /locus_tag="ACHKV2_001186"
     CDS             15387..16889
                     /gene="araA"
                     /locus_tag="ACHKV2_001186"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO6939110.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17089..17784
                     /gene="araD"
                     /locus_tag="ACHKV2_001187"
     CDS             17089..17784
                     /gene="araD"
                     /locus_tag="ACHKV2_001187"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6939111.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17859..20210
                     /gene="polB"
                     /locus_tag="ACHKV2_001188"
     CDS             17859..20210
                     /gene="polB"
                     /locus_tag="ACHKV2_001188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO6939112.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20374..23280
                     /gene="rapA"
                     /locus_tag="ACHKV2_001189"
     CDS             20374..23280
                     /gene="rapA"
                     /locus_tag="ACHKV2_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO6939113.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23292..23951
                     /gene="rluA"
                     /locus_tag="ACHKV2_001190"
     CDS             23292..23951
                     /gene="rluA"
                     /locus_tag="ACHKV2_001190"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO6939114.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24164..24342
                     /locus_tag="ACHKV2_001191"
                     /pseudo
     CDS             24164..24342
                     /locus_tag="ACHKV2_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24369..25121)
                     /gene="espY1"
                     /locus_tag="ACHKV2_001192"
     CDS             complement(24369..25121)
                     /gene="espY1"
                     /locus_tag="ACHKV2_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFO6939115.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNATLDGATLDGATVDGATVDGATHLYDEVI
                     IINKITPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(25416..26231)
                     /gene="djlA"
                     /locus_tag="ACHKV2_001193"
     CDS             complement(25416..26231)
                     /gene="djlA"
                     /locus_tag="ACHKV2_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO6939116.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26486..28840
                     /gene="lptD"
                     /locus_tag="ACHKV2_001194"
     CDS             26486..28840
                     /gene="lptD"
                     /locus_tag="ACHKV2_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO6939117.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28893..30179
                     /gene="surA"
                     /locus_tag="ACHKV2_001195"
     CDS             28893..30179
                     /gene="surA"
                     /locus_tag="ACHKV2_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO6939118.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30179..31168
                     /gene="pdxA"
                     /locus_tag="ACHKV2_001196"
     CDS             30179..31168
                     /gene="pdxA"
                     /locus_tag="ACHKV2_001196"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO6939119.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31165..31986
                     /gene="rsmA"
                     /locus_tag="ACHKV2_001197"
     CDS             31165..31986
                     /gene="rsmA"
                     /locus_tag="ACHKV2_001197"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO6939120.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            31989..32366
                     /gene="apaG"
                     /locus_tag="ACHKV2_001198"
     CDS             31989..32366
                     /gene="apaG"
                     /locus_tag="ACHKV2_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO6939121.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32373..33221
                     /gene="apaH"
                     /locus_tag="ACHKV2_001199"
     CDS             32373..33221
                     /gene="apaH"
                     /locus_tag="ACHKV2_001199"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO6939122.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(33218..33532)
                     /locus_tag="ACHKV2_001200"
     CDS             complement(33218..33532)
                     /locus_tag="ACHKV2_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO6939123.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33535..33768)
                     /locus_tag="ACHKV2_001201"
     CDS             complement(33535..33768)
                     /locus_tag="ACHKV2_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO6939124.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33854..34333)
                     /gene="folA"
                     /locus_tag="ACHKV2_001202"
     CDS             complement(33854..34333)
                     /gene="folA"
                     /locus_tag="ACHKV2_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO6939125.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34525..36387)
                     /gene="kefC"
                     /locus_tag="ACHKV2_001203"
     CDS             complement(34525..36387)
                     /gene="kefC"
                     /locus_tag="ACHKV2_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO6939126.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36380..36910)
                     /gene="kefF"
                     /locus_tag="ACHKV2_001204"
     CDS             complement(36380..36910)
                     /gene="kefF"
                     /locus_tag="ACHKV2_001204"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO6939127.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37018..38349)
                     /gene="yaaU"
                     /locus_tag="ACHKV2_001205"
     CDS             complement(37018..38349)
                     /gene="yaaU"
                     /locus_tag="ACHKV2_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939128.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38407..38694)
                     /gene="fixX"
                     /locus_tag="ACHKV2_001206"
     CDS             complement(38407..38694)
                     /gene="fixX"
                     /locus_tag="ACHKV2_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO6939129.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38691..39977)
                     /gene="fixC"
                     /locus_tag="ACHKV2_001207"
     CDS             complement(38691..39977)
                     /gene="fixC"
                     /locus_tag="ACHKV2_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6939130.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40028..40969)
                     /gene="fixB"
                     /locus_tag="ACHKV2_001208"
     CDS             complement(40028..40969)
                     /gene="fixB"
                     /locus_tag="ACHKV2_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6939131.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40984..41754)
                     /gene="fixA"
                     /locus_tag="ACHKV2_001209"
     CDS             complement(40984..41754)
                     /gene="fixA"
                     /locus_tag="ACHKV2_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO6939132.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            42228..43742
                     /gene="caiT"
                     /locus_tag="ACHKV2_001210"
     CDS             42228..43742
                     /gene="caiT"
                     /locus_tag="ACHKV2_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO6939133.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43773..44915
                     /gene="caiA"
                     /locus_tag="ACHKV2_001211"
     CDS             43773..44915
                     /gene="caiA"
                     /locus_tag="ACHKV2_001211"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO6939134.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            45044..46261
                     /gene="caiB"
                     /locus_tag="ACHKV2_001212"
     CDS             45044..46261
                     /gene="caiB"
                     /locus_tag="ACHKV2_001212"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO6939135.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46335..47888
                     /gene="caiC"
                     /locus_tag="ACHKV2_001213"
     CDS             46335..47888
                     /gene="caiC"
                     /locus_tag="ACHKV2_001213"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO6939136.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47997..48782
                     /gene="caiD"
                     /locus_tag="ACHKV2_001214"
     CDS             47997..48782
                     /gene="caiD"
                     /locus_tag="ACHKV2_001214"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO6939137.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48788..49378
                     /gene="caiE"
                     /locus_tag="ACHKV2_001215"
     CDS             48788..49378
                     /gene="caiE"
                     /locus_tag="ACHKV2_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO6939138.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49497..49892)
                     /gene="caiF"
                     /locus_tag="ACHKV2_001216"
     CDS             complement(49497..49892)
                     /gene="caiF"
                     /locus_tag="ACHKV2_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO6939139.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(50153..53374)
                     /gene="carB"
                     /locus_tag="ACHKV2_001217"
     CDS             complement(50153..53374)
                     /gene="carB"
                     /locus_tag="ACHKV2_001217"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO6939140.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53392..54540)
                     /gene="carA"
                     /locus_tag="ACHKV2_001218"
     CDS             complement(53392..54540)
                     /gene="carA"
                     /locus_tag="ACHKV2_001218"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO6939141.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13122 a        14779 c        14311 g        12654 t
ORIGIN      
        1 gcaacgcgtg cattttgtcc cctttttcct cgtggattag atgcaaaaat ttattctgaa
       61 atatgtcaac cgatgaaaag catcggtagt taagcagaaa ttaatatcgc ttgttttaac
      121 cacagcagca caattagcta atcttacgga tgcagaactc atgctggcgg gacgttttta
      181 ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata tcgcattcat
      241 ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta ctaaacgcat
      301 cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg cagtcaaaca
      361 aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc taaaagagac
      421 aaggacccaa accatgagcc agcaagtcat tattttcgat accacattgc gcgacggtga
      481 acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc tggcccttga
      541 gcgtatgggt gttgacgtga tggaagtcgg gttccccgtc tcttcgccgg gtgattttga
      601 atcagtgcaa accatcgctc gccaggttaa aaacagccgt gtatgcgcgt tagctcgctg
      661 cgtggagaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag ccttccgtat
      721 tcataccttt attgccactt cgccaatgca catcgccacc aagctgcgca gcacgctgga
      781 cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt
      841 tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc gagtggtcga
      901 agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg gctacaccat
      961 gccgtttgag ttcgccggaa tcattagcgg cctgtatgaa cgcgtgccta acatcgacaa
     1021 agccattatt tccgtacata cccacgacga tttgggcctg gcggtcggca actcactggc
     1081 ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc
     1141 cggtaactgt tccctggaag aagtcatcat ggcgattaaa gttcgtaagg atattctcaa
     1201 cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag ttagccagat
     1261 ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcggcgcat tcgcacactc
     1321 ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc
     1381 agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc
     1441 ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt tagacaattt
     1501 gtacgacgcc ttcctcaagc tggcggacaa aaaaggtcag gtgtttgatt acgatctgga
     1561 ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc tggattactt
     1621 cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg cctgcggcga
     1681 agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct atcaggcgat
     1741 taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca
     1801 cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc gccgcttcca
     1861 cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg tgcacgtact
     1921 taacaatatc tggcgtgcca cagaagtcga aaaagagttg caacgcaaag ctcaacacaa
     1981 cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt attgccgggg
     2041 gatggcattg gtccggaagt gatgacccag gcgctgaaag tgctggatgc cgtgcgcaac
     2101 cgctttgcga tgcgcatcac taccagccat tacgatgtag gcggcgcagc cattgataac
     2161 cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg
     2221 tttggctcgg taggcggccc gaaatgggaa catttaccac cggaccagca accagaacgc
     2281 ggcgcgctgt tgcctttgcg taagcacttc aaattattca gcaacctgcg tccggcaaaa
     2341 ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc aaacggcttc
     2401 gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc aaaaggccgc
     2461 gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg ttttgagatc
     2521 gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccataa agtgacgtcg
     2581 atcgataaag ccaacgtgct gcaatcctct attttatggc gtgaaatcgt caacgagatc
     2641 gccacggaat acccggatat cgaactggcg catatgtaca tcgacaacgc caccatgcag
     2701 ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt
     2761 ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc cgccagcctg
     2821 aacgagcaag gttttggact gtatgaaccg gcgggcggct cggcaccaga tatcgcaggt
     2881 aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct gcgttacagc
     2941 ctggatgccg atgatgcggc ttccgccatt gaacgcgcca ttaaccgcgc attagaagaa
     3001 ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga tgaaatgggc
     3061 gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaagacg ttatacgaaa
     3121 aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg
     3181 accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg
     3241 gtcgcccggt acgtcagccg ggcaaaacct tcgccaccat ggatcacaac gtctctaccc
     3301 agactaaaga cattaatgcc tgcggtgaaa tggcgcgcat ccagatgcag gagctgatca
     3361 aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccctat caagggatcg
     3421 tccacgtaat ggggccggaa cagggcgtga ccttgccggg gatgaccatt gtctgcggcg
     3481 actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg
     3541 aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgtgcgaag accatgaaaa
     3601 ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg ttggcaatta
     3661 tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa
     3721 tccgtgattt aagcatggaa ggtcggatga ccctgtgcaa tatggcaatc gaaatgggcg
     3781 cgaaagccgg tctggttgca cctgacgaaa ccacctttaa ctatgtcaaa ggccgtctgc
     3841 atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg
     3901 acgaaggcgc aactttcgat accgttgtca ctttgcaggc agaagagatt tcaccgcagg
     3961 tcacctgggg aactaaccca ggccaggtga tttccgtgaa cgacaatatt cccgatccgg
     4021 cttcgtttgc cgatccggtt gaacgcgcgt cggcagaaaa agcgttggcc tatatggggc
     4081 tgaaaccggg tattctgctg accgaagtgg ctatcgacaa agtgtttatc ggttcctgca
     4141 ccaactcacg tattgaagat ttacgcgcgg cggcggaaat cgccaaaggg cggaaagtcg
     4201 cgccaggcgt acaggcgctg gtggttcccg gctctggtcc ggtaaaagcg caggcggaag
     4261 cagaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg cctggctgct
     4321 caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt gcctccacca
     4381 gcaaccgtaa ctttgaaggc cgtcaggggc gcggcgggcg cacgcatctg gtcagcccgg
     4441 caatggctgc cgccgcggct gtgaccggcc atttcgccga cattcgcaac attaaataag
     4501 gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc gctggatgcc
     4561 gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt gacccgtact
     4621 ggttttggcg cgcatctgtt taacgactgg cgttttctgg atgaaaaagg ccaacagcca
     4681 aacccggact tcgtgctgaa cttctcgcag tatcagggcg cttccatttt gctggcacga
     4741 gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac cgactacggt
     4801 tttaaggtgg tgattgcgcc gagttttgct gacatcttct acggcaatag ctttaacaac
     4861 cagctgctgc cggtgaaatt aagcgacgca gaagtggatg agctgtttgc gctggtgaaa
     4921 gccaatccgg ggattcattt tgatgtagac ctggaagcgc aagaggtgaa agcgggtgag
     4981 aaaacctatc gctttaccat tgatgccttc cgccgccact gcatgatgaa cggtctggac
     5041 agcattgggc ttaccttgca gcacgacgac gccattgcgg cttatgaagc gaagcaaccc
     5101 gcgtttatgc gttagtttgt acaccgtccg atactcaatc gccaaaaaga aaaaagccag
     5161 cagataatca ctgctggcgt ggcgattaac accaatactc agtcacacat gatgtccgca
     5221 aatcagtagt cagaatgact cccctcccac tgcatcagtt cttcaagcat ggttaatcgt
     5281 tgtgggacgc ttaaccaacg caaccagcac aacttcgctg tcgcggtaaa atagtgctga
     5341 taaaactgac gaatggggca cctccttgct tcatcgttag gaattattga cttaatatag
     5401 ggaaaataaa attgctgtct tttgcgcagg agttcccctt ttatgccatc tgctcgtctg
     5461 caacaacagt tcatccgcct gtggcaatgc tgcgagggta aatcgcagga cacaacgctc
     5521 aacgaactgg cagcgttatt gagctgctcg cgtcgtcata tgcgtaccct gctcaacacc
     5581 atgcaggatc gcggctggct gacgtgggaa gcggaagtcg ggcgtggtaa acgctcgcgt
     5641 ctgacattcc tctataccgg gctggcgctt cagcaacagc gggcggaaga cctgctggag
     5701 caggaccgta tcgaccaact agtgcagtta gttggcgaca aagcgactgt gaggcaaatg
     5761 ctggtttctc atctgggccg cagcttccgc caggggcggc acatcctgcg cgtgctctac
     5821 tatcgtccgt tgcgtaatct gctacctggc agcgcattgc gccgttccga aacccatatc
     5881 gcccggcaaa tcttcagttc gctaacgcgc ataaatgagg aaaatgggga actggaagca
     5941 gacatcgccc accactggca gcaaatatca ccgcttcact ggcgtttctt tttgcgtcca
     6001 ggagtccatt ttcatcatgg tcgtgaactg gaaatggacg acgtgatcgc ctctttaaaa
     6061 cgaatcaata cgctgccgct ctattcgcat attgctgaca ttgtgtcgcc gacgccctgg
     6121 acgctggata tccacctcac gcagccggat cgctggttgc cgttactgct gggacaagtt
     6181 ccggcgatga tcctgccgcg cgaatgggaa accctcagta actttgccag ccatcccatc
     6241 ggcaccggtc cgtatgcggt gattcgcaac agcaccaatc aactgaaaat tcaggcattc
     6301 gatgacttct tcggttaccg ggcattaatc gatgaagtta acgtctgggt tctgccggaa
     6361 attgccgacg agccagccgg agggctgatg ctaaaaggtc cacagggcga ggaaaaagag
     6421 attgaaagcc gcctggagga aggttgctac tatttactgt tcgatagccg cacccatcgc
     6481 ggggcgaatc agcaagtcag ggactgggta agctatgtgc tttctccaac taatctggtc
     6541 tatttcgctg aggaacagta ccagcaactg tggttcccgg cttatggact gctcccccgt
     6601 tggcatcatg cccgcaccat aacgagcgaa aaaccggctg gtctggaaag cctcaccctg
     6661 accttttatc aggatcacag tgagcatcgg gtgattgccg ggatcatgca gcagattctg
     6721 gcaagccacc aggtcacact ggaaatcaaa gagatcagct acgatcagtg gcatgaagga
     6781 gagatcgaga gcgatatctg gcttaacagc gccaacttta cgctgccgct ggatttttcg
     6841 ctgttcgcgc acctgtgcga ggtaccgctg ctccaacact gtattccaat cgactggcaa
     6901 gtcgacgccg cccgctggcg caatggcgaa atgaacctgg cgaactggtg ccaacaactg
     6961 gtcgccagca aagcaatggt gccacttatc caccactggc tgatcattca gggacaacgc
     7021 agtatgcgcg gcctgcgcat gaacaccctc ggctggtttg attttaaatc agcgtggttt
     7081 gcgccgccgg atccagagta gcttgcgcct tttggcggtc atgagcgcat tttttatatt
     7141 cagacggaat gataaaatac attactttta tattttctct gttttatgca tcgtctgatt
     7201 agcgtcgcaa ataagattaa agaaggggca ttccagcccc tctttcatat tctgagttaa
     7261 tcaataacgg cgcagtctag ccttgttctg gccggaaagg tcagcttttt caggttaacc
     7321 tcgcgcaggt cagtcatacc aacggctatt gttcgtttca gcattgctca ggtcaacctc
     7381 gagacagtca ataccacaaa tgacaacccc gttaaagtaa gcattactca gatcaatccc
     7441 cccggtcctg gtgctctttt tcagctgcaa ctccagagta aatatcgcct catcgccaat
     7501 aaaaaaactg acgcataacg taccttctgg cgagagtgag gccttcgcgg ccatattttc
     7561 cggatctttt gcgtattctt tgagttcatt aaattttatc agccagtgag ttaacttatc
     7621 acgcgccttc ccgtcgacag ccccggtaaa gtattttgag tagtcatcac tattctggaa
     7681 ataaaatgca tccttcagaa tttgccttgc cgcagtctgg gaatgtttat ctgactggaa
     7741 gagattgcgt aacgtctccc atagctcttc aaagatattc atattctcgg tctttttgac
     7801 caagttagcc atcctgataa tctgagaatt agtaaggcct ggatctttag tggtattact
     7861 agcagggata ctaatctgtt ctgggtttct tactttcatg ctgatagctc cactctgaat
     7921 aaaaaactca gaataagcgt cctgaataag aatactgtca aaaatcgctc attcacgcaa
     7981 taaacggtta cgttacctat tttgactgta gcgaagggat tctttcggat caacgctctc
     8041 cagctatgca aatacgctct tgatagctga acgaatattg gcttttttaa tacgccttct
     8101 ctatggattg ctggttgata acaaaatcac tacactaacg ccgttctcaa cggggtgcca
     8161 cgcggacgcg tgcgctgaga aaatacccgt cgaacctgat ccggataacg ccggcgaagg
     8221 gatttgaggc tccttctcaa gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa
     8281 aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg
     8341 tttataccta cgattccttc gccgccgact gggggcctgg tccggtggtt aaaaaagcct
     8401 ttgaagccga ctgtaattgc gaactgaaac tggtggcgct ggaagatggc gtttcgcttc
     8461 tcaaccgtct acggatggaa ggcaaaaaca gtaaagccga tgtggtgctg gggctggata
     8521 acaatctgtt agacgccgcc agtaaaaccg gactgtttgc caaaagcggt gtggcagcgg
     8581 atgccgttaa cgttcccggc ggctggaata atgacatttt cgtaccgttt gattatggct
     8641 acttcgcctt cgtttatgac aagaacaaac tgaaaaaccc gccacaaagc ctgaaagaac
     8701 tggttgagag cgatcaaaac tggcgggtga tttatcagga tccgcgcacc agtacaccgg
     8761 ggctgggtct gttgctatgg atgcaaaaag tctatggcga tgacgcccca caagcctggc
     8821 agaaactggc gaagaaaacg gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt
     8881 ttttaaaagg tgaaagcgat ctggtactga gttacaccac ctctccggct tatcacattc
     8941 tcgaagagaa gaaagataac tacgccgccg cgaacttcag cgaaggtcac tatctgcaag
     9001 tggaagtcgc cgctcgcacc gctgccagca agcagccgga gctggcgcaa aaattcctcc
     9061 agtttatggt ttctccggct ttccagaatg cgatcccaac cggcaactgg atgtatccgg
     9121 tggcaaacgt cacgctgcct gccggttttg aaaaattgac caaacccgca accacgctgg
     9181 agttcacgcc agccgaagtg gcggcacaac gtcaggcatg gattagcgaa tggcaacgcg
     9241 ccgtcagccg ttaattcccg gctggttaat tccaggtgta agcgccgcca cgctggtggt
     9301 agcggttgcg ctggcggcgt ttctcgccct gtggtggaac gcgccgcagg gtgactggtc
     9361 ggcagtctgg caggacagct acctgtggca tgtggtgcgc ttctccttct ggcaggcgtt
     9421 tctctcggcg ctactctctg tcgtacccgc gatattcctc gcccgcgcgc tctatcgcag
     9481 gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca atgaccttga tcctcccggt
     9541 gctggttgct gttttcggca ttcttagcgt ctatggtcgc cagggctggc tggcatcgct
     9601 ctgccaatcg ctcggtctgg agtggacctt ttcgccctac ggcctgcaag gcattttgct
     9661 ggcccatgtg ttttttaatc tgccgatggc gagccgctta ttactccagg cactggaaaa
     9721 cattcccggc gaacaacgtc agcttgccgc ccagcttggg atgcgcggct ggcatttttt
     9781 ccgcttcgtc gaatggccgt ggttacggcg acaaatcccg ccggttaccg cgcttatctt
     9841 tatgctctgt ttcgccagct tcgccaccgt gttatcgctg ggcggcggtc cgcaggcaac
     9901 cactatcgag ctggcaatct atcaggcgct gagttacgac tacgatcctg cccgcgcggc
     9961 gatgctggcg ctgatccaga tggtgtgttg ccttgggctg gtgctgttga gtcagcgact
    10021 gagtaaggcc attgcgcccg gcaccacgct gctgcaaggc tggcgcgacc cggacgatcg
    10081 tctgcatagc cgcatttgcg acacgatgtt aattgtgctg gcgctgctgc tgttgctgcc
    10141 accgttgctg gcggtgatcg tcgatggggt aaatcgccag ttgccggaag tgctggcaca
    10201 accggtgctg tggcaggcgc tgtggacctc gttgcgtatt gcgctggcgg caggtgtatt
    10261 gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc gaactgcggg cgcggcaaaa
    10321 aatgctggcg ggccaggcgc tggagatgag cggcatgttg atcctcgcta tgccggggat
    10381 tgtgctggca acgggcttct ttttactgct caacaacact atcggcctgc cacaatctgc
    10441 tgacggcatt gtgattttca ccaatgcgtt aatggcgatc ccctacgccc tgaaagtgct
    10501 ggaaaacccg atgcgcgata tcaccgcccg ctacagcatg ttatgtcagt cgctggggat
    10561 tgaaggctgg tcacgcttaa aagtagtgga gctgcgcgcc ctgaaacgcc cgctggcgca
    10621 ggcgctggcc tttgcctgcg tgctgtcgat tggtgatttt ggcgtggtgg cgttgttcgg
    10681 taacgatgat ttccgcaccc tgccgtttta tctctaccag caaattggct cctatcgcag
    10741 ccaggacggc gcggtcaccg cgttaattct gctgctgctc tgttttctgc tgtttagcgt
    10801 gattgaaaaa ataccggggc gaaatgttaa aactgactga tatcacctgg ctttatcacc
    10861 atttgccgat gcgttttagc ttaacggtag aacgcggcga gcaggtggcg atcctcgggc
    10921 caagcggcgc gggtaaaagt accctgctga acttgatcgc cggttttctg acaccagcca
    10981 gcggtgcgct gactatcgac ggcgttgatc acaccacgac gccgccgtca cgccgtccgg
    11041 tgtcgatgct gtttcaggag aacaacctgt tcagccacct gacggtcgcg cagaacatcg
    11101 ggctggggct taatccagga ttgaagctga acgcggtaca gcaggagaaa atgcacgcga
    11161 ttgcccgtca gatggggatt gataatttaa tggcgcggtt accgggcgag ctttccggcg
    11221 gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtacg cgaacagccg attttattgc
    11281 tcgatgaacc gttctctgcg ctcgatccgg cgctacgtca ggagatgttg acgctggtga
    11341 gcactagctg ccagcagcaa aaaatgacgc tattgatggt gtcgcacagt gtggaagatg
    11401 cggcgcggat tgccacgcgc tcggtagtag tcgccgacgg gcgaatcgcc tggcagggta
    11461 tgaccaatga gttgttgagc ggtaaggcga gtgcttcggc gctgttggga attacaggtt
    11521 aatatgcact gtcggatgcg gcgtgaacgc cttatccgac ctacgggtgc gttttgtccg
    11581 catcaagcgc cccacacaag actatgccct aaactccaac cactttacgc aaaatatcga
    11641 tatacaccgg cattaacgga tggcgaacta acaccaccag cgccaccacg ccgatggcag
    11701 aaatcaacgg cgtcagccac aacaaacgac cgcgggacaa ataatgactc aaacgatcgg
    11761 ttgctttgcc gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg
    11821 ccgtcgccag cagcaaccat ttaaactcac cgctctgcat tccggctgga atatcgatcg
    11881 ccgcacccgc cagaatcccc ggcaggaagt aaaacggtgg ccacagcagg cagccgataa
    11941 tattcggcgt gataaatttt gccaccggga gatccagcat tcccgccacc attggcacca
    12001 gcggacgcgt cgggccaaca aaacgaccga ccagaatggt gaacatgctg tgttgatgca
    12061 acgcatgttc agttttatcg agtaatgctt tgttcttctt cagaaatgac caacgatgca
    12121 atggcttttt aaaacgccag cccagccaga aggagatcca gtcgcccagc agacaaccga
    12181 cgatccctgc cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca
    12241 gccccgccat cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa
    12301 aggccaccaa caccaccgcc atcaatgaat acacggtgga ttgggtaata aagtgttcca
    12361 gcaatgcttg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc
    12421 tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca
    12481 tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt
    12541 ttaaatactc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg
    12601 ataggcatcc gggtagtgct caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc
    12661 cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac
    12721 aggcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg
    12781 atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc
    12841 gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag
    12901 cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg
    12961 tgcgcttcat ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca gttaagccat
    13021 tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc
    13081 ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg
    13141 cagacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga
    13201 atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc attggcctca
    13261 atcggcgtta aacccgccac cagatgggcg ttaaacgagt atcccggcag caggggatca
    13321 ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca
    13381 tattgcatca gatattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg
    13441 gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg
    13501 cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgataattt gcacggcgtc
    13561 acactttgct atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt
    13621 ttatcgcaac tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc
    13681 gattgcaatt ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcac
    13741 caccggtgaa gaaatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt
    13801 ttgtgatgcc ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga
    13861 agcggcactg aaaaccgtgc ttgcagaact tagcgtcgaa cagcgcgcag ctgtggtcgg
    13921 gattggcgtt gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct
    13981 ggcgctgcgc ccggagtttg ccgaaaaccc gaacgcgatg ttcgtattgt ggaaagacca
    14041 cactgcggtt gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaatgttga
    14101 ctactcccgc tatattggcg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca
    14161 tgtgactcgc caggacagcg ccgtggcgca atctgccgca tcgtggattg agctgtgcga
    14221 ctgggtgcca gctctgcttt ccggtaccac gcgcccgcag gatattcgtc gcggacgttg
    14281 cagcgccggg cataagtcgc tatggcacga aagctggggc ggcctgccgc cagccagttt
    14341 ctttgatgag ctggatccga tcctcaatcg ccatttgcct tccccgctgt tcactgatac
    14401 ctggactgcc gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct
    14461 gcctgaaagc gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggtgc
    14521 aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat
    14581 tgccgacaaa cagagcgttg gcgagcgggc agtgaaaggt atttgcggtc aggttgatgg
    14641 cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta
    14701 cgcctggttc ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga
    14761 actaaaagag caaatcgacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg
    14821 ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggccgccg
    14881 cacaccgaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga
    14941 cgctccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat
    15001 ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc
    15061 gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat
    15121 tgttgcctct gaccaatgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa
    15181 agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct
    15241 gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct atcagcaatg
    15301 ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc
    15361 cgttccgact ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg
    15421 taattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg
    15481 agcacgttgt gaaagcgctg aatacggaag cgaaactgcc ctgcaaactg gtactgaaac
    15541 cgctgggcac cacgccggat gaaatcaccg ctatttgccg tgatgctaat tacgacgatc
    15601 gttgcgctgg tctggtggtg tggctacaca ccttctcccc ggccaaaatg tggatcaacg
    15661 gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac gcggcgctgc
    15721 cgtgggacag catcgatatg gactttatga acctgaacca gaccgcgcac ggcggtcgcg
    15781 agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc
    15841 aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggta tctaaacagg
    15901 atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg gcggtcaccg
    15961 atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg
    16021 gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg
    16081 agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga
    16141 acgtgctgga agcggcgcgt attgagctgg ggatgaaacg tttcctggaa caaggtggct
    16201 tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt cctggtctgg
    16261 ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaactg
    16321 ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta
    16381 tggaggacta cacctatcac ttcgagaaag gcaatgacct ggtgctcggc tcccatatgc
    16441 tggaagtctg cccgtcgatc gccgcagaag agaaaccgat cctcgacgtt cagcatctcg
    16501 gtattggtgg taaggacgat cctgcccgcc tgatcttcaa tacccaaacc ggtccagcga
    16561 ttgtcgccag cttgattgat ctcggcgatc gttaccgtct actggttaac tgcatcgaca
    16621 cggtgaaaac accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc
    16681 aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg
    16741 tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg cacgacattg
    16801 aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgcg ctgcgctgga
    16861 acgaagtgta ttacgggttt cgccgctaag tagccgcatc cggtatgtaa cgcctgatgc
    16921 gacgctggcg cgtcttatca ggcctacacg ctgcaatttt gtaggccgga taagcaacgc
    16981 gcatccggca ttcagcgcct gatgcgacgc tgacgcgtct tatctggcct acacgctgcg
    17041 attttgtagg ccggataagc aacgcgcatc cggcacgaag gagtcaacat gttagaagat
    17101 ctcaaacgtc aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc
    17161 acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc
    17221 ggcgtcgatt acagcatcat gaccgctgac gatatggtcg tggtcagcat cgaaaccggt
    17281 gaagtggttg aaggtgcgaa aaagccctcc tccgatacgc caactcaccg actactctat
    17341 caggcattcc cgtccattgg cggcattgtg cacacgcact cgcgccacgc caccatctgg
    17401 gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc
    17461 accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa
    17521 accggtaacg tcatcgtaga aaccttcgaa aaacagggta tcgatgcagc gcaaatgccc
    17581 ggcgttctgg tccattctca cggcccattt gcatggggca aaaatgccga agatgcggtg
    17641 cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg tcagttagcg
    17701 ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg taagcatggc
    17761 gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca aatgaaacca
    17821 ggctataatc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct
    17881 taacccgaca ctggcgggat accccgcaag gaacagaggt ctccttctgg ctggcgacgg
    17941 acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcccgccg
    18001 atcaggttcc ccgcgctcag catattttgc ggggtgaaca aggctttcgc ctgacgccgc
    18061 tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc
    18121 agttgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac gaggccgatg
    18181 tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg
    18241 gtgatatgca caatggcgct atcgttaatg cccgtctgaa accgcatccc gactatcgtc
    18301 cgccgctcaa gtgggtttct attgatattg aaaccacccg ccacggagag ctgtactgca
    18361 tcggcctgga gggctgcggg cagcgcatcg tttatatgct gggaccagag aatggcgacg
    18421 cctccgcgct cgattttgaa ctggagtacg tcgccagccg cccgcagcta ctggaaaaac
    18481 tcaacgcctg gtttgcaaac tacgatcctg atgtgatcat cggttggaac gtggtgcagt
    18541 ttgatctgcg aatgctgcaa aaacatgccg agcgttatcg tattccgctg cggctggggc
    18601 gcgataacag tgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc
    18661 aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt
    18721 tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg
    18781 ataacccgtg ggatcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc
    18841 tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccat aaaactgaaa
    18901 tcatgccgtt tttgctcgaa cgagcaacgg tgaacggcct gccggtggat cgacacggcg
    18961 ggtcggtggc agcgtttggt catctctatt ttccccgtat gcatcgcgct ggttatgtcg
    19021 cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcgc
    19081 gacctgggct ttatgattcg gtgctggtgc tggactacaa aagcctgtac ccgtcgatca
    19141 tccgcacctt tctgattgat cccgtcgggc tggtggaagg catggcgcag cctgatccag
    19201 agcacagcac cgaaggcttt ctcgatgcct ggttctcgcg agaaaaacat tgcctgccgg
    19261 agattgtgac taacatctgg cacggacgcg atgaagccaa acgccagggt aacaaaccgc
    19321 tgtcgcaggc gctgaaaatc atcatgaatg ccttttatgg cgtgctcggc accaccgcct
    19381 gccgcttctt cgatccgcgg ctggtatcgt cgatcaccat gcgtggtcat cagatcatgc
    19441 ggcaaaccaa agcgttgatt gaagcgcagg gctacgacgt gatctacggc gataccgact
    19501 caacgtttgt ctggctgaaa ggcgcacatt cggaagaaga agcgacgaaa atcggtcgtg
    19561 cactggtgca gcacgttaac gtctggtggg cagaaacgct gcaaaaacaa cagctgacca
    19621 gcgcactgga actggagtat gaaacccatt tctgccgttt tctgatgcca accattcgcg
    19681 gagccgatac cggcagtaaa aagcgttatg ccgggctgat tcaggagggc gacaaacagc
    19741 ggatggtgtt taaagggctg gaaaccgtgc gtacggactg gacgccgctg gcccagcagt
    19801 ttcagcagga gctttacctg cgtatcttcc gcaacgagcc atatcaggag tacttccgcg
    19861 aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac cgtaaacgcc
    19921 ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc gccgctcgtc
    19981 ttgccgatga agaaaaccaa aagcgcggtc gccccttgca atatcagaac cgcggcacca
    20041 ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg
    20101 attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta
    20161 ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaag
    20221 agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc
    20281 ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat
    20341 tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg
    20401 atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact
    20461 gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc
    20521 gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg
    20581 caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat
    20641 actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc
    20701 aaaccgcagg accgcctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt
    20761 tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt
    20821 cagcgtacca gcctgatccc gcaccagctc aacatcgctc atgatgttgg ccgccgccac
    20881 gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg
    20941 atccttcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa
    21001 accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgcgctg
    21061 tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt cgacaccgaa
    21121 cagctggtga tttgctcgct ggattttgcc cgccgtagca aacagcgtct ggaacatctc
    21181 tgtgaagccg agtgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa
    21241 gacgcgccga gccgcgaata tcaggctatt gaacaactgg cagagcacgt gccgggcgtt
    21301 ctgctgctga ccgcaacccc ggaacagctg ggaatggaaa gccacttcgc ccgtctgcgt
    21361 ctgctggacc cgaaccgttt ccacgatttc gcgcaattcg ttgaagagca gaaaaattat
    21421 cgtccagtag cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa
    21481 ctgaacatgc ttggcgagat gatcggcgag caggatatcg agccgctgtt gcaagcagca
    21541 aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat
    21601 cgccacggca ccagccgcgt gctgttccgt aacacccgta acggtgtgaa aggcttcccg
    21661 aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa
    21721 gtctccggca ttatgggcgc acgtaaaagt gcggaagacc gcgcccgcga tatgctctac
    21781 ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg
    21841 cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc
    21901 tgcgctaaag ctgccactgc gctgcaactg gagcaagtac tgcgcgaacg tgaaggtatt
    21961 cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg
    22021 tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga
    22081 cgtaacttcc agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta
    22141 ctggagcagc gtattggtcg tctggatcgt atcggccaag cgcacgatat tcagatccat
    22201 gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg
    22261 gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat
    22321 ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaaac
    22381 tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg
    22441 gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag
    22501 gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat cggtatcaat
    22561 caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg
    22621 gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg
    22681 gcgcgtgaag atgcgcagtt tattacctgg gaacatccgc tgatccgcaa cggtctggat
    22741 ctgattcttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg
    22801 ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag
    22861 ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc
    22921 aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac
    22981 cgtcacaccg gcagcaaact ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa
    23041 ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc acgtaacgaa
    23101 gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg
    23161 aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc
    23221 ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa
    23281 cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc
    23341 tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg tctgtgccgg
    23401 gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgagat tatccgcagg
    23461 cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca
    23521 aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgtgccgaaa aagcagtatg
    23581 tgacccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg ccgctgattt
    23641 gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga
    23701 cggaatatga agtggtggag tatgcggcgg ataacacggc aagagtggtg ttaaaaccga
    23761 ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg
    23821 gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg
    23881 cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag
    23941 cggattttta acctttcctg tacgtttata ccctcatcct aagggctgcc tcttgtacat
    24001 aaatccccga gtccggggat tttttgtttc tgccaaacag ctttcagttg ttgcgtgtgg
    24061 ctacgctttc cattcggtaa ttcccttcct gctccacagc gaatttttga actttttctt
    24121 cacccgttga ccgtctctta tatttcattt tatcgggtct gttatggtcc tttctgattg
    24181 ttattcatgg gctaattagc agtctggttg gcgatgccca tctcggtgat ccgcgtcgta
    24241 cacgacgcct tgcgtcacta gcctcttctc tagctcatca tgcaggtctt tctaattgtg
    24301 aaatcatccc acgctactgc taaggttgaa ggggcgttcc agccccttca accttagctt
    24361 attctggctc attcaataat tgcgttgtca gtaatttgtt cagcagtact ttgtttagta
    24421 gcaacttctt cagtatcaat ttttttgggg gtgattttat taataataat tacctcatca
    24481 tataggtggg tagcaccgtc cacggtagca ccgtccacgg tagcaccgtc cagggtagca
    24541 ccgtccaggg tagcattcca cccaggttcc aagtaaagga ctttattgtg acctttcatt
    24601 gttttgagac caaggtaaca atccctcatg tcaacgccgg gaaggtaagc ctctctaaga
    24661 tcaacgccca agttacctgg gcctttatgc agctgcaacg tctgagtaca tatctcctta
    24721 ttttcaataa aaaaagtgac acttaactcg atatcaccga aggtctgtgc gacccagctc
    24781 atattgccct ttgcgaccat ctcatccgga ttttttgcgc atgttttgag ttcattgaat
    24841 aattccagac tatggataaa cttttctttc tcattctcgt tgacagcccc agtaaagtat
    24901 tttgaggcgt tgatgttgcc agccttttta ttcacgaaat tgaacagaag tttataagcc
    24961 tcagtctgtt tatctgacca gaacagatga cgtaacgtct cccacaagcg ttgaaagata
    25021 ttcatcttca tgtccatctt gtccccgctc accatcttat aaatatcgtt attgctcata
    25081 tttaaaagtg tagccggcat gcctggaact gatactttca tggtaatagc ctcgcattga
    25141 gttaaaaact catgatgcgc gctctgaata aatatacttt caaaaatcgc tcattcagat
    25201 atgtgttttt tatttttaag acgtaaaaat ataattccgc tcgccgtaaa gctctcaacc
    25261 ttaagcaggg tcaacgcgat aaaaccaaca gaaaccctcc cgtaaaccgt atattgagcc
    25321 tgtccaccat tctgagtaaa cgccttttct caaagaagtg accgccaggg cggccacctt
    25381 ttatcgtggg cagttactca gcatcattta cagggtcatt taaacccttt ctgctgctta
    25441 atcagctcat aggcctgctg aatttcctgc gctttctgct tcgccatctc catcatctca
    25501 ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt cactcatcag cttacggtag
    25561 gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt acaggcatct
    25621 tccagcgttg ggccacgctg cgcttgctgc cagttaccac cgccagattg ctgctgataa
    25681 ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg
    25741 gagatcccta attcttcagc aatgacatac agcaccgccc gttcattcgg gtgcagtgaa
    25801 ccatcagcaa acgccgcctg aatctggatc tccagaaaca tacgaattaa gtcaaaacga
    25861 ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt
    25921 cccacccgga acgcattttg cgccgcagta cgggaagcgc catgaagatt cattcggtcc
    25981 atcaactggc tggcgatatg aatatcagcc tccgtgacgc gacctttgga tttggttaaa
    26041 tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg
    26101 aaccacgcca ttttacggct acgggcttta tcaaacatat ggccaattaa caggcctaac
    26161 actacgcccc aaaagccgcc gcccatcagt aaggccacgg ccacgccaat gatttttccc
    26221 caatactgca tatattcccc aaatcgacac acggatatca gggctatctc ccacaatata
    26281 aaggtgcttt taccgttttc cggctgcggt caattgtggg acatcgccta taatttgcat
    26341 tatcatacct gtcattcaat gccgtgccta acaccactga cgctattcgg acaggattaa
    26401 cactagcgta gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc
    26461 gatgatggaa caataaaatc aacgtatgaa aaaacgtatc cccactctcc tggccaccat
    26521 gattgccacc gccctttata gtcaacaggg actggcagcc gacctcgcct cacagtgcat
    26581 gttgggcgtg ccaagctatg accgtcctct ggtacagggc gataccaatg acttacccgt
    26641 gactatcaat gctaaccacg cgaaagggga ctacccggat gacgccgtgt ttactggcag
    26701 cgtggatatc atgcagggta acagccgtct gcaggccgac gaagtgcagc tccatcaaaa
    26761 agaggcacca ggacaaccgg agccggtacg taccgttgat gcgctcggta atgtccatta
    26821 cgacgataac caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga
    26881 taccaacgtc tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga
    26941 cctgatgaaa caacgtggcg aaaaccgcta taccattctg gataacggta gctttacctc
    27001 ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc gaaattattc atgaccgcga
    27061 agaacaagtt gcggagatct ggaacgcccg ctttaaggtg ggtccggtat cgatctttta
    27121 tagcccctat ttgcagttgc cggtgggtga caaacgtcgc tctggtttct tgatcccgaa
    27181 cgccaagtac accaccacca actactttga gttctacctg ccatattact ggaacatcgc
    27241 gccaaatatg gatgccacca tcacgccgca ttatatgcat cgtcgtggca acatcatgtg
    27301 ggagaacgaa ttccgctacc tctcccaggc gggcgctggc ttgatggaac tggactatct
    27361 gccttcagat aaagtctatg aagatgaaca cccgaacgat gacagttcac gtcgttggtt
    27421 attctactgg aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac
    27481 caaggtcagc gatcctagct acttcaatga tttcgataac aagtacggtt ccagtactga
    27541 cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca atgccaccgt
    27601 ttcaaccaag cagttccagg ttttcagcga acagaacacc agtagctact cggcagagcc
    27661 gcagttagac gttaattact accagaatga tgttggtccg tttgatacgc gtatttacgg
    27721 ccaggcagtg cactttgtta acaccagaga cgacatgcct gaagcaaccc gtgttcacct
    27781 ggaaccgacc atcaatttgc cgctctctaa taactggggc agcatcaata ccgaagcgaa
    27841 gttgctggca acccattatc agcaaaccaa tcttgactgg tataactcca gaaacacgac
    27901 caagctggac gaatccgtta accgcgtaat gccgcaattc aaagttgacg gcaaaatggt
    27961 ctttgaacgc gatatggaaa tgctggctcc gggttatacc caaacgctgg aaccgcgcgc
    28021 gcagtatttg tacgtgccgt atcgcgatca gagcgacatc tataactacg actcgtctct
    28081 gctgcaatct gactactctg gcctgttccg ggaccggact tacggcggtc ttgaccgtat
    28141 tgcctccgct aaccaggtga cgaccggtgt cacatctcgc atatatgatg atgctgccgt
    28201 tgaacgtttt aatatttccg ttggtcaaat ctactatttc acggagtctc gcactggcga
    28261 tgacaacata acatgggaga atgacgacaa aacgggttca ctggtgtggg caggcgatac
    28321 ttactggcgt atctccgagc gttggggatt gcgtggcggg attcagtacg atacacgtct
    28381 ggataacgta gcgaccagta actccagcat tgaataccgt cgggatgaag accgtctggt
    28441 acagctgaat taccgttacg ccagcccgga atatattcag gctacgctgc ctaagtacta
    28501 ttccactgct gagcaatata agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc
    28561 aattgccgat cgttggtcca ttgttggggc ctactactac gacaccaatg cgaacaagca
    28621 agccgactct atgttaggtg tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta
    28681 cgagcggaag ctgaacggtt gggataacga taaacaacat gcggtatatg acaacgcaat
    28741 cggctttaac atcgaacttc gcggcctgag ctccaactac ggtctgggta cgcaagagat
    28801 gctgcgttcg aacattctgc cgtatcaaaa cactttgtga tttgttgatt taccacgtaa
    28861 tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg
    28921 gtatcgccat gatcgcgaat accagtttcg ctgcccccca ggtagtcgat aaagtcgcag
    28981 ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga tggattaatg cagtcggtaa
    29041 aactgaacgc tgctcaggca aggcagcaac ttcctgatga cgcgacgctg cgccaccaaa
    29101 tcatggaacg tttgatcatg gatcaaatca tcctgcagat ggggcagaaa atgggagtga
    29161 aaatctccga tgagcagctg gatcaggcga ttgctaacat cgcgaaacag aacaacatga
    29221 cgctggatca gatgcgcagc cgtctggctt acgatggtct gaactacaac acctatcgta
    29281 accagatccg caaagagatg attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca
    29341 tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg
    29401 ccagcactga gctgaacctg agccacatcc tgatcccgct gccggaaaac ccgacctctg
    29461 atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg
    29521 ctgatttcgg taagctggcg attgctcatt ctgccgacca gcaggcgctg aacggcggcc
    29581 agatgggctg gggccgtatt caggagttgc ccgggatctt cgcccaggca ttaagcaccg
    29641 cgaagaaagg cgacattgtt ggcccgattc gttccggcgt tggcttccat atactgaaag
    29701 ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat gctcgccata
    29761 ttctgctgaa accgtcgccg atcatgactg acgaacaggc tcgtgtgaaa ctggaacaga
    29821 ttgctgctga tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc
    29881 aggatccagg ctctgctaac cagggcggtg atctcggctg ggctacacca gatattttcg
    29941 atccggcctt ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc
    30001 actcttcatt cggctggcat ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg
    30061 acgctgcgca gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc tcggaagaag
    30121 cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg agcaactaat
    30181 ggttaaaacc caacgtgttg tgatcactcc cggcgagccc gccgggattg gcccggactt
    30241 agttgtccag cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac
    30301 tctccttacc gaccgggcag cgatgctcgg tttgccgctc accctccgcc cttattcccc
    30361 caactcccct gcacaaccgc aaactacggg cacattaacg ctacttcctg tcgcgctacg
    30421 tgaatctgtc actgcggggc agttagcgat tgaaaatggg cattacgtgg tggagacgct
    30481 ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct gcgctgatca caggccccgt
    30541 gcataaaggc gtcattaacg acgcaggcat tccgtttacc ggtcataccg agtttttcga
    30601 agagcgttcg caggcgaaaa aagtggtgat gatgctggcg accgaagaac ttcgcgtggc
    30661 gctggcaacg acgcatttac cgctgcgcga tatcgcagat gctatcaccc ctgcactttt
    30721 gcacgaagtg attgctattt tgcatcacga tttgcgtaca aaatttggta ttgccgaacc
    30781 gcgcattctg gtctgcgggc tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga
    30841 agagatagac accattattc ctgtgctcga cgagctgcgg gcgcagggga tgaaactcaa
    30901 cgggccgctg cctgccgata ccctgtttca gccgaaatat ctcgataacg ccgacgccgt
    30961 gctggcgatg taccacgatc agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg
    31021 tgtgaacatt acgctgggcc tgccctttat tcgcacatca gtggaccacg gcaccgcact
    31081 tgaactggcg ggacgtggcg aagccgatgt cggcagtttt attacggcgc ttaatctcgc
    31141 catcaaaatg attgttaaca cccaatgaat aatcgagtcc accagggcca cttagcccgt
    31201 aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga tcgacagcat tgtttctgcc
    31261 attaacccgc aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc ggcattgacc
    31321 gagccggtcg gcgaacgtct ggaccagctg acggtcatcg aacttgaccg cgatctggcg
    31381 gcacgtctgc aaacgcatcc attcttaggc ccgaaactga cgatttatca gcaggatgcg
    31441 atgaccttta actttggcga actggccgag aaaatgggtc agccgctgcg tgttttcggc
    31501 aacctgcctt ataacatctc cacgccgttg atgttccatc tgtttagcta tactgatgcc
    31561 attgccgaca tgcactttat gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg
    31621 aacagcaaag cgtatggtcg attaagcgtc atggcgcaat actattgcaa cgtgatcccg
    31681 gtactggaag ttccgccgtc agcctttaca ccaccaccca aagtggattc cgccgtcgtg
    31741 cgcctggttc ctcatgcaac gatgcctcac ccggttaaag atgttcgtgt gttgagccgc
    31801 atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg
    31861 tttagcgtcg aggtgttaac gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc
    31921 tctgtcgcgc aatattgcca gatggcgaac tatctggcgg agaacgcgcc ttttcaggag
    31981 agttaacgat gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg
    32041 cacaatcttc acctgataat gaacgttacg tttttgctta taccgtaacc atacgcaatc
    32101 tggggcgagc gccagtgcag ttgttggggc gttactggct gatcaccaat ggcaatggcc
    32161 gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg
    32221 aagagtacca gtacaccagc ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc
    32281 actacgaaat gatcgatgaa aatggcgtcc ctttcagcat cgacattccc gtattccgac
    32341 tcgccgttcc cacactcatt cattaaaaga atatggcgac ataccttatt ggcgacgttc
    32401 atggttgtta cgatgaactg atcgcattgc tgcataaagt agaatttacc cctgggaaag
    32461 ataccctctg gctgacgggc gatctggtcg cgcgcggccc gggttcgctg gatgttctgc
    32521 gctatgtgaa atccttaggc gacagcgtac gtctggtgct gggcaatcac gatctgcatc
    32581 tgctggcagt atttgccggg atcagccgca ataaaccgaa agatcgcctg acaccgctgc
    32641 tggaagcgcc ggatgccgac gagctgctta actggctgcg tcgccagcct ctgctgcaaa
    32701 tcgacgaaga gaaaaagctg gtgatggccc acgcagggat cacgccgcag tgggatctgc
    32761 agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct gtcgagtgac tcctatccct
    32821 tctttctcga cgctatgtac ggcgatatgc caaataactg gtcaccggaa ttgcgggggc
    32881 tgggaagact gcggtttatc accaacgcct ttacccgtat gcgtttttgc ttcccgaacg
    32941 gtcaactgga tatgtacagc aaagaatcgc cggaagaggc ccctgcccca ctgaaaccgt
    33001 ggtttgcgat tcctggacct gtcgctgaag aatacagcat cgcctttggt cactgggcat
    33061 cgctggaagg caaaggtacg ccggaaggta tttacgcgct ggataccggc tgctgctggg
    33121 gtgggtcatt aacctgcctg cgctgggaag ataaacagta ttttgtccag ccgtcgaacc
    33181 ggcataagga tttgggtgag ggagaggcgg tcgcgtctta aatcccgtcg agcataaaat
    33241 ctaatgctcc tttaatcaac gttcggtgcg cgctggcatc gcaaaatttc gttcccagcg
    33301 cgttaactgg aacagttgca gtctcatgcg tcatgagcac atattctttg ccgtctacca
    33361 gtaaaaggat tgggttaagc cgttctggtg ggcgaatacg gctaaatcgt tcaataggtg
    33421 ttaatggaat aacaatacgg cgattaatca caccaataat gtcgctggta acatcgataa
    33481 caaagggaaa cgcggcgttc ctgctgcgac tgcggtatac cgtaaattgc atcgctagaa
    33541 gctccggtac tcatcagaga aacatccggt ttcatcagcc aattgattta acgcagcgat
    33601 agcttcagcg ttctcttcac gccaacgtgt tgctgcatgt tttttaagtt cagcattgag
    33661 cgcaaccgta agggtggcgc tcatatttaa gcctgcatca cgagcgcgat tgactaactc
    33721 acggtcgacg gtgacggtca cactttgtgt ggtacgtttt gcagtcatga tatcgtgctc
    33781 ctgtacaatt tatacgtcta gtatacacgg atatctgcac gtatcagtga tgccggaatt
    33841 ctaatataca aaattaccgc cgctccagaa tctcaaaaca atagctgtga gagttctgcg
    33901 catcagcgtc gtggaattcg ctgaataccg attcccagtc atccggctcg taatccggaa
    33961 aatgggtgtc gccttccact tctgcgtcga tatgcgtcag gtacagtttt tgcgctttcg
    34021 gcaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt acgtcaccac
    34081 acgccgcgat ggcttcatcc accgacttca cccacgttac gcgatcgtcc gtacccggtt
    34141 gactgctgag gataatattt ttgcgtcctg gcaacggacg accgatggat tccctggtat
    34201 ggcggcccat aattacgggt ttatttaagg tgttgcgttt aaaccaggcg agatcggcag
    34261 gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct aacgccgcaa
    34321 tcagactgat cattgagatt tcccgataaa aaaaattgtc gccactatac gtaaagcgta
    34381 aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct gctggcgagt
    34441 ccacgctctc tccctggact cgccgcatta caatgaaaca aaaacaaaca gttagctgta
    34501 aagtgtgatt tacgtcactc tttattagga tgagggtttc gtttccggtt catccgccat
    34561 gttgccggta tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag
    34621 atgttcgcgg tcctcggtga tgatctcact taacatcgcg ctggtgcgtt tatagaccgc
    34681 cgcgcgggct ttggtgtcat tctcaaccat tgccatctct tccaccatct gaatattaaa
    34741 gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag
    34801 cgccagacgc ccggttttca gcgcaccttc aaaggtttca cgctctggct tttcaacgcc
    34861 tgcctgacgc aaacaaatgt agtgatcgac atcgcgggcg cgggcaataa tctgcaaatg
    34921 cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat cgtcaatggc
    34981 gttaatcagc acttccgctt tcgccgctcc cgcagattcc agtaaatcca tccgcgtggc
    35041 atcgccataa aacactttca taccaaattt acgcagcgtt tcgatatggt ccggatcgtg
    35101 atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtga tctgcccgaa
    35161 acgaccgaat ccggcaatga ttacccgcgg ctgttcttcg tcgatctcat cggcttcacg
    35221 cgcctcttca gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttgc
    35281 cgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt
    35341 cgccatctgc gccgcgccaa ataccacaaa ggcaaactca ctgccctgcc ctaacaacac
    35401 cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt cgggcaatca gccacagcat
    35461 ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaat
    35521 cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc
    35581 tttaaagggt tcgatatcgc tttccagcgc atgacggtat tccgagcttg ccagcagtac
    35641 gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa
    35701 gccaaacaca aggaataacg ccacggcact aaacacttcc cgcaagccag agcgggcaac
    35761 aaaacgcagc gccggacgcg tgacatagcg ccccagcagt accaccagca ccagcgcgcc
    35821 cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc
    35881 cagtagcggg atcatcgcca ccagcgggat tgccgcgata tcctggaaca gcagcaccgc
    35941 aaaggcactg cggcccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc
    36001 aatcgccgta gaggagagcg ccagcgtcat gccgatcaat tccgcgacct gccagcgcag
    36061 cccaagtaac atacagaaca gccccagcag gccgccgcaa atcaccatct gcaatgcgcc
    36121 accgccaaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt cgaggccgat
    36181 aataaacagc atcagcacca cgccaatctc ggcaaagtgc agaatagatt cggcatcggt
    36241 caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc caagtaccga
    36301 tcccagacca agacgtactg caatgggtac aatcagcgcc gccgaaccga gataaatcag
    36361 cgcctgaacc agcgtatggc tatccatgat gggcctcctg ccattccagc agacgttgct
    36421 tatagtgacg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg
    36481 caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca
    36541 gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg ccggtcgtca
    36601 ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt
    36661 gcgagaagac tttatcgatc caaagtttaa ggagcggagg aatgctgtac cactgcatcg
    36721 ggtgctgcca gacgatcaga tcggcgcgag acagcgcctc ctgctcggcg gcaatatcga
    36781 tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc agcgtccttg
    36841 cctgttcaaa catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatta
    36901 taagaatcat aggtagcccg cgtgtttacg cctttgtttt cccacaaagt gtagtcatat
    36961 attcacgcgg cttatagtca ataatattga ttcagaaaag agataaatct gaacaaatta
    37021 ccccattctc tgcccgcgga tcgtcatatt gctggtctgc gccagtgaca tccctcgagt
    37081 ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg cccccattag
    37141 catcgtgtta ctgataccgt aattattgat aaagatcggt agtgcccagg tcgaaacaat
    37201 ggtgccaata cgacttaagg acataatcac gcccacggca gaggcgcgga tatccgtcgg
    37261 gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga aaaaggcata
    37321 caccgcaaag gccatcacta ccagccagat ccccatatcc gggatcagcc ccaaaaccgc
    37381 cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt
    37441 tagccacagc atcggcggaa tacagccgag cataaagaac aggctaatca ccacgttccc
    37501 cagtgccgcg tttttgccaa cacccaatcc caacaaacca acgatttgcg ggccaaaggt
    37561 gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa acagaacaaa
    37621 aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct gctcttcatc
    37681 gaaagccacc ggttcgccaa acagtttgat catcatctct tcgcactctt ttactcgccc
    37741 tttgcgtaat aaccagcggg gagattcagg cagttcgaat cgaccaatca aaatcaacaa
    37801 acaggggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat cataaagcca
    37861 gtagccgacc agatcggcac aggtcgcgcc gacataccac atggcggcga taaagctgat
    37921 ggaaaacgcc cgctgacggg tactggagaa ctcagtgatc atcgaagtgg cgatgggata
    37981 gtctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga cgggggatga
    38041 aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga ggaacatttt
    38101 gcgccgtccg actttatcgg aaatataacc aaacagcgat gtgccaacga acagcccggc
    38161 gagcgttccc gcgcccagca agccaatcca gtcagcgtcc agttttagcg ccggcgtcag
    38221 ttgctccagc gccacgccaa tcattaccag tatataacca tccagaaacg gaccaccgct
    38281 tccccacagc aaaatgcggc ggtgaataga ggaaaattta agatcgtcaa agtttctgga
    38341 cggttgcatg ggttattcct gttttttgca atgagagtca gtgcggcgtt acgtatcaaa
    38401 ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc
    38461 cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc
    38521 gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag
    38581 cagctccagc gcctgtttat cggcatcggc tttcacaaca atgtgcggat gctcttcatc
    38641 gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg
    38701 gtcactcctt tcatgccatc cttgatcaga ttgatgaagc ccactttctt gccgtggcgg
    38761 aggattttct tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc
    38821 acgcctaccg ccagttccgg gtagccgcta aacatgcgtg ggttatcaag gaaggccggt
    38881 agtttctggt acatacgcat atcgcgcagc gggccactct caagatgctg acgatattcc
    38941 gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca
    39001 gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac
    39061 attccggcgg catcaccggc aatcaatacg ccgtcaccga ccagctccgg cagcatgttg
    39121 atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccacc cgcgatcagc
    39181 ggtgcaacgg ccggatgttg tttgaaatct tccagcattt gcggcaccga ttttttcgcg
    39241 tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtgttttc attggtataa
    39301 agaaaaccgc cgcccatcag gccatcggtg ggtgatcccg caaataggca agccgccccc
    39361 tgattaccct gcaactgaaa acggtcttca ataaccgact tcggtaactc gatcagttcc
    39421 ttcacgccaa ccgccacatt cgtcggttta acgcgttttg ccatccccag cttttcggca
    39481 aggatggagt tcactccatc agcaaggatc accgttttcg cttcaatcac atcgccatcg
    39541 gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta
    39601 attaactgcg cgcccgcttc ttcggcctgc tccatcagcc aggcatcaaa tttactgcgc
    39661 aaaacggagt aagaacgctg ggatggcgag gtttcgtcac cgttgcagta gtccatagtc
    39721 atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctaccggg
    39781 gcggattcgg cgaaaccagg aataatgtgt tccaggctgt gggcatagag acgcccgccg
    39841 gtgacgttct tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgccccttcg
    39901 cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg caccgacgat gatggcgtca
    39961 aagatatctt cggacataag agctccctgt cagcggcagg accaaaatgc agccctgcca
    40021 gagtggatca acgcgctaaa gctgcggtca gcgccggaag gatcttcacg gcgtcgccaa
    40081 caatgccgta atccgcgtac tggaagatcg gcgcattttt atctttattg atggcgaaaa
    40141 tggtttgcga cgcgttagcg ccaaccatgt gctggatctg cccggagatc cccacagcca
    40201 ggtacagttc aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc
    40261 atttttcgtt ttctgccacc ggacgagaac aggccaattc cgcacctatc gccttgcaaa
    40321 gctgttctgc cagcgcaatg ttctctttgc tgccaatacc gcgaccgacg ctgaccacca
    40381 ggcgggcttt gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa
    40441 tcgccacagc cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg
    40501 gctgagccgc atcgaacgtg ccgctgctga tggtcagtac cgcatacggc gtggcaatgc
    40561 gttcttcgcc aatcgccaga ccaccgtaaa ccatatgttt cactgttgct ttaccgtcct
    40621 gtacgctgac ggtgctggca tcgttagaca ccgccgcttt aaggcgataa cccagttttg
    40681 ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac caggccgtct gcaccgtgct
    40741 ggcgaatagt gtcagccatg acactggcgt aatcttcgat catccgatcg tccggtttgc
    40801 cgtttaattt ccagacatga ttagcgccga gctggattgc ctgtgcgccg tcggcatcat
    40861 tgaggacaaa ggcattgatt tgattagcta aagcctgcgc accgttcatc agttccggca
    40921 gacgagaagg ggtatcgctg aatacccaga cttgagaaaa cgtgttcata gcatcccctg
    40981 taattaaatg actttgcgaa gattttcagc aaatgcggcg atctgttctt cgccgtcgcc
    41041 ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg gcaacctgtt gttctgacca
    41101 tgctgcctct gcgttaaaac cgatatccgc cgccgaccat acctggactg gctttttcgc
    41161 cgcgccgaga atggctttca tcgaaggaat ttgtggggag ttgatatcag tggaaacagc
    41221 aacaaccgca ggcagtggaa tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac
    41281 ggtgagggta tctgccgtca gggagataat tttgctgacg ccgttaactg ccggaatatt
    41341 gaggatttcg cctaccagca aaccaacctg ctgggcataa aggtcggaag aaccatcgcc
    41401 acagaggatc aaatcaaagc ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg
    41461 ttgcggcagt gcctgctcga actggtcatc aatcaccaca atcagttcat ccggaccgcg
    41521 cgatagcaca tctttacgcc ctttggcgtt ggtcagggct ttaccgccca cacttagggc
    41581 cgtcacctgc gcctctgctg cctgttgctt tagctggcaa gccgcttcaa tagcgttgag
    41641 atcgtattgg cttattttgg catcggcttt actgaagtct aacgaaccat cagcattatt
    41701 gaccgcaata tcctgttcat caggcacgca cttatagcaa gtaataatct tcattgcatc
    41761 tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg ttaattacag atatcatgcc
    41821 ctttaatttg gaaataaaac tttaatcacc aatattgaaa atgtcacacg cagataaatc
    41881 caactttcaa tattgttaag ttcctcacca atattgaaaa cacggcgtag caaaaagaaa
    41941 ttttcaatat tgttttatgg atcaccaata ttgaaagtca gatgctattt ttttactcgc
    42001 aacataacat aacaaactga ttaataatca aaattaacga gaaaagcgcc ctctattcac
    42061 ccgaaacaaa aatgtgataa caatcacaga atgcagctta ttgaataccc attatgagtt
    42121 agccattaac gcgtccatga ggttaataat aattatatta aatgttaaca aaaataaaac
    42181 aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa
    42241 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc attaataatc gtcggcatac
    42301 tttgttggct tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca
    42361 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
    42421 gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccacctg
    42481 aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
    42541 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
    42601 cgaactcgac aggagcgaaa gagttggggc tggcttacag cttgttcctc tggggacctc
    42661 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
    42721 aaatggaagt gattcgcccc agctccaccc tggtgccgct ggtaggtgaa aaacacgcca
    42781 aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
    42841 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
    42901 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
    42961 tttgcgtcgc ctgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
    43021 tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact
    43081 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg
    43141 atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg
    43201 tgatttacgc catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc
    43261 gtgaactgtg cttcggcatg gtgatgggac tgacagcatc cacctggatc ctgtggactg
    43321 tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt ccaaatctga
    43381 tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccgctcagca
    43441 ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg
    43501 cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt gaagaaccac
    43561 cgctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttttgctgg
    43621 cgctcggcgg cctgaaaccg attcaaactg ccattatcgc cggaggatgc ccgctgttct
    43681 tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt
    43741 aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg
    43801 aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg
    43861 ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg
    43921 gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc
    43981 tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagt
    44041 tgccgggcgg gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta
    44101 tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg
    44161 gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta
    44221 atggtagtaa gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc
    44281 gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac
    44341 cgggcatcaa agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa
    44401 tcacctttga cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct
    44461 ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg
    44521 gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc gtgcagtttg
    44581 gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat
    44641 taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca
    44701 cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg
    44761 atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct
    44821 tctggcgtga cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga
    44881 cgctgggtcg tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc
    44941 tgacgcgtct tatcaggcct acgggagcac gaatgtaggc cggataaggc gtttacgccg
    45001 catccggcag tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat
    45061 gccgaaattc gggccgttgg ccggattgcg cgttgtcttc tccggtatcg aaattgccgg
    45121 gccgttcgcc gggcaaatgt tcgcagaatg gggtgcggaa gttatctgga tcgagaacgt
    45181 cgcctgggcc gacaccattc gcgttcaacc gaactacccg caactctccc gccgcaattt
    45241 gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat
    45301 ggaaaccacc gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat
    45361 taccgatgaa gtactgtggc aacacaaccc gaaactggtt atcgctcacc tgtccggttt
    45421 tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc
    45481 cttcagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta
    45541 taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa
    45601 agcgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg
    45661 tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag
    45721 caaaggtaaa gatccctact atgccggttg cggtctgtat aaatgtgccg acggctacat
    45781 cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc
    45841 acatctactc ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg
    45901 cccttacggc ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc
    45961 agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc
    46021 ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat
    46081 ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca
    46141 aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg
    46201 ctacagcgaa aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta
    46261 aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa
    46321 tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc
    46381 tggcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta
    46441 accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata
    46501 cgctggggat tcgcaaaggc gacaaggttg cactacatct cgacaactgc ccggaattta
    46561 tcttttgctg gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc
    46621 tgttacgcga ggaaagtgcg tggatcctgc aaaatagcca ggcgtgcctg ctggtgacca
    46681 gtgcgcaatt ctatcctatg tatcaacaga ttcagcaaga agatgccact caattgcggc
    46741 acatttgcct gacagatgtg gcacttcccg ctgatgatgg cgtgagttcg tttactcaac
    46801 tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg
    46861 cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt
    46921 acaacctgcg cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg
    46981 tctacctgac ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg
    47041 cgttttctgc cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg
    47101 gacaggtaca gaagtaccgc gccaccatta ccgaatgtat tccgatgatg attcgtacgt
    47161 tgatggtgca gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt
    47221 atctcaactt gtcggagcag gaaaaagaca cattttgtga acgcttcggt gttcgcttgc
    47281 tgacgtctta tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctggcgata
    47341 aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta cgacgcggag atccgcgacg
    47401 atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag
    47461 ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcaaaa gtgctggaag
    47521 ctgatggctg gctgcatacc ggcgataccg gataccgcga cgaagaaggc tttttttatt
    47581 tcatcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc
    47641 tggaaaatat catcgccacc catccaaaaa ttcaggatat cgtggttgtg ggtattaaag
    47701 attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga
    47761 gcgaagagga atttttccgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt
    47821 atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc
    47881 tgaaataaat gaaacggcag ggaactaccc tgccagctaa taatcacgct ctaaaacaat
    47941 atgcattttt tgcagggatg ttgtcgtccc tgaaaaagca aaaatggaga aaaggaatga
    48001 gtgaatcatt acatctgacc cgcaatggat caattctgga aattactctt gatcgcccaa
    48061 aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc
    48121 gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg
    48181 cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg
    48241 gtggttttgc gggattaacc gaaattttca atctcgacaa accggttatc gcagctgtga
    48301 acggctacgc ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg
    48361 ccgataacgc cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg
    48421 gtgtgctgcg tctgccgaag atcctgccgc ctgccatcgt caatgaaatg gtgatgaccg
    48481 gcagacgaat gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc
    48541 aggcggaact gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc
    48601 tggcgattgc ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaat
    48661 cgtatagcta tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag
    48721 atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt
    48781 aacaaccgtg agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt
    48841 tgtccatccc agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg
    48901 cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat
    48961 tcaggatggc tgcattatgc atggctacac cgacactgac accatcgttg gggaaaacgg
    49021 ccatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg
    49081 gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg ttgccgccat
    49141 gagctttgtc aaagcaggct ttcacggcga gaaacgccag ttgttgatgg gtacgccagc
    49201 ccgcgctgta cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga
    49261 gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacacagc cactgaggca
    49321 aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc
    49381 ttgccggatg cggcgtaaac gccttatcca actctgctgg agagcaaccg taggccggat
    49441 aagatgcgcc agcatcgcat ccggcgacca ggtcgcacgg tatcatcgaa atgacattaa
    49501 cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag ttccgtgagc
    49561 ggaacggcag gaatacgcgg tgtctttctt acgccgacta atttttcccg actgttattg
    49621 cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt taaccagtcg ctgacactgg
    49681 catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat ttctgtgact
    49741 tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc gaaatgaata
    49801 gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc gattaacaag
    49861 tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca aattaaaagt
    49921 ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt ggatatatcg
    49981 atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt catcaaaaat
    50041 atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt
    50101 attagttatt cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga
    50161 tctgtgcgtg catttcctgc accgaaatta ctttttcagt cgcatcggca ttcagcgcca
    50221 tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac
    50281 tgcgacggat cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg ttgatgatgt
    50341 aggtatattc gccattcttg atacggtcct gaatgtgcgg acggccttca tgcaccttgt
    50401 ttaccagacg cggattgata cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat
    50461 ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc
    50521 cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg ctgcccagct
    50581 gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catgacttcc ccggtagaac
    50641 gcatttctgg ccctaacagc gggtcaacgc ccgggaattt attgaacggc agcaccactt
    50701 ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt
    50761 tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga
    50821 acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca
    50881 ccgcaaactg cacgttcatc aggccgcgca cctgcaattc gaaggccagt ttctgcacct
    50941 gctggcgcat tacatcctga atttcctgac ttaaggtgta cgctggcagc gaacatgcgg
    51001 agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca
    51061 tttcgccgtc gcagatggca tccacgtcaa cttctactgc gtcatcaagg aaatgatcca
    51121 gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga cgcaggtcag
    51181 cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgcaccacca
    51241 gcgggtagcc aatctctttt gccttctcaa ctgccatttc aatagcagta acggtggcgt
    51301 tcgccggttg tttcagtttc agacggtcaa ccgcatgctg gaagcgttca cggtcttccg
    51361 cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcggct tccagtgcac
    51421 gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa
    51481 tacgcacgat ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag
    51541 tgtcgtagtc ggtagagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt
    51601 cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc gaattcgata ccctgaccga
    51661 tacggtttgg accgccgcca agcaccatga ttttttcacg gtcggtagac ggattcgctt
    51721 cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac tctgccgcac
    51781 aggtatccac gcgcttgtag accgggtgca ggtcatattg gtcacgcagc ttacggattt
    51841 ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct ttgcgtttca
    51901 gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgccact ttctcttcca
    51961 gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatgttagtc aggttgaaga
    52021 cgccgtccac agacaggccc gcgcggaaag catcggcgat gtaccagata cgctctgcgc
    52081 ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcc aggctcactt
    52141 tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct
    52201 gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt
    52261 cgttagcacc ggcgaatttt tcgaagttga agcgaggaat tttggtaacc acgtaatcga
    52321 tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcatcga
    52381 gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg
    52441 acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt
    52501 ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca
    52561 gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg
    52621 gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc
    52681 agacgatgat gcagttgtcg tttttatcgc gcaccacttc catctcgtac tctttccagc
    52741 cgatcagcga ctcatcaatc agcaactctt tggtcggaga gagatccaga ccgcgggcgc
    52801 aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa
    52861 aggatgggcg aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca
    52921 tcgtatgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga
    52981 aacgacggcg gtcttctgct ttatcaatcg catcggcagt ggcaccaatc atggtgacgc
    53041 cgaactcttc caacacgccc tgacgttcca gctccagcgc gcagttcagc gccgtctgac
    53101 cgcccatggt tggcagcacc gcgtccgggc gctctttttc aataatctta cgtaccactt
    53161 cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg
    53221 ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt
    53281 gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa
    53341 tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc
    53401 ggttttacgg tactgctcaa ttaactcgat aaagtggtcg aacaacggcg cggcgtcgtg
    53461 tggaccaggg ctggcttcag ggtgaccctg gaagctgaac gccggtttat cggtgcgatg
    53521 aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa
    53581 tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc
    53641 cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc
    53701 accgctcgcc agcgccagca gctgatgacc gaggcagatg ccgaagaccg gaatatcggt
    53761 ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc
    53821 gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc
    53881 cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgtttggc
    53941 accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag
    54001 gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc
    54061 ttttgccaga tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccagcgc
    54121 cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa
    54181 acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta
    54241 agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac
    54301 cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc
    54361 aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc
    54421 ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc
    54481 tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa
    54541 aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta
    54601 aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg
    54661 ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta ttttgtggct catgcatgtt
    54721 ttacaggcat taagcgacaa aaagatcatc taacctgttc tggaaaacgc ttgcgcagca
    54781 agaagattgc atgaaataaa aacgagcagg caaaagtgga ccaaatggtc aaaatttaca
    54841 ttaagacaac aaaaatagaa gacaaa
//
LOCUS       DAZFTN010000017        47582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFTN010000017 DAZFTN010000000
VERSION     DAZFTN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            670..2013
                     /locus_tag="ACHKV2_001219"
     CDS             670..2013
                     /locus_tag="ACHKV2_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939142.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(2162..3670)
                     /gene="putP"
                     /locus_tag="ACHKV2_001220"
     CDS             complement(2162..3670)
                     /gene="putP"
                     /locus_tag="ACHKV2_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO6939143.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3829..4038)
                     /locus_tag="ACHKV2_001221"
     CDS             complement(3829..4038)
                     /locus_tag="ACHKV2_001221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939144.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            4093..8055
                     /gene="putA"
                     /locus_tag="ACHKV2_001222"
     CDS             4093..8055
                     /gene="putA"
                     /locus_tag="ACHKV2_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6939145.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(8095..8733)
                     /gene="rutR"
                     /locus_tag="ACHKV2_001223"
     CDS             complement(8095..8733)
                     /gene="rutR"
                     /locus_tag="ACHKV2_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO6939146.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            9021..10112
                     /gene="rutA"
                     /locus_tag="ACHKV2_001224"
     CDS             9021..10112
                     /gene="rutA"
                     /locus_tag="ACHKV2_001224"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO6939147.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            10112..10804
                     /gene="rutB"
                     /locus_tag="ACHKV2_001225"
     CDS             10112..10804
                     /gene="rutB"
                     /locus_tag="ACHKV2_001225"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO6939148.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10816..11202
                     /gene="rutC"
                     /locus_tag="ACHKV2_001226"
     CDS             10816..11202
                     /gene="rutC"
                     /locus_tag="ACHKV2_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO6939149.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            11210..12010
                     /gene="rutD"
                     /locus_tag="ACHKV2_001227"
     CDS             11210..12010
                     /gene="rutD"
                     /locus_tag="ACHKV2_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO6939150.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            12020..12610
                     /gene="rutE"
                     /locus_tag="ACHKV2_001228"
     CDS             12020..12610
                     /gene="rutE"
                     /locus_tag="ACHKV2_001228"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO6939151.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12621..13115
                     /gene="rutF"
                     /locus_tag="ACHKV2_001229"
     CDS             12621..13115
                     /gene="rutF"
                     /locus_tag="ACHKV2_001229"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO6939152.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            13136..14464
                     /gene="rutG"
                     /locus_tag="ACHKV2_001230"
     CDS             13136..14464
                     /gene="rutG"
                     /locus_tag="ACHKV2_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO6939153.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14547..14654)
                     /locus_tag="ACHKV2_001231"
     CDS             complement(14547..14654)
                     /locus_tag="ACHKV2_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFO6939154.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            15422..15495
                     /locus_tag="ACHKV2_001232"
                     /pseudo
     CDS             15422..15495
                     /locus_tag="ACHKV2_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(15585..23966)
                     /locus_tag="ACHKV2_001233"
     CDS             complement(15585..23966)
                     /locus_tag="ACHKV2_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO6939155.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYNDSAVWVAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTVSAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     TKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADRERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDITHNVRMPQPDQLPRTVRNSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLASEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDKASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGTNLFFSESRDELLTKAK
                     VWFAELEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSVLLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGAGASSQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKL
                     SRDNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKSMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRSGAVDITPESLKYWVDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYVDQQKMYDRMTEVAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKS
                     VERDMKIAVDRFNREYNKKVGVD"
     gene            complement(24036..25301)
                     /locus_tag="ACHKV2_001234"
     CDS             complement(24036..25301)
                     /locus_tag="ACHKV2_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939156.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            complement(25312..25563)
                     /locus_tag="ACHKV2_001235"
     CDS             complement(25312..25563)
                     /locus_tag="ACHKV2_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO6939157.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(25573..26019)
                     /locus_tag="ACHKV2_001236"
     CDS             complement(25573..26019)
                     /locus_tag="ACHKV2_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939158.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(26022..26678)
                     /locus_tag="ACHKV2_001237"
     CDS             complement(26022..26678)
                     /locus_tag="ACHKV2_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939159.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(26772..27173)
                     /locus_tag="ACHKV2_001238"
     CDS             complement(26772..27173)
                     /locus_tag="ACHKV2_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939160.1"
                     /translation="MAPVSGERMNDKILWYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTHLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(27230..27370)
                     /locus_tag="ACHKV2_001239"
     CDS             complement(27230..27370)
                     /locus_tag="ACHKV2_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6939161.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(27603..28337)
                     /locus_tag="ACHKV2_001240"
     CDS             complement(27603..28337)
                     /locus_tag="ACHKV2_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6939162.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(28428..29045)
                     /locus_tag="ACHKV2_001241"
     CDS             complement(28428..29045)
                     /locus_tag="ACHKV2_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939163.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(29051..29329)
                     /locus_tag="ACHKV2_001242"
     CDS             complement(29051..29329)
                     /locus_tag="ACHKV2_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO6939164.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINDNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(29344..30612)
                     /locus_tag="ACHKV2_001243"
     CDS             complement(29344..30612)
                     /locus_tag="ACHKV2_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6939165.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(30609..32234)
                     /locus_tag="ACHKV2_001244"
     CDS             complement(30609..32234)
                     /locus_tag="ACHKV2_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939166.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPASSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVINFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(32468..32980)
                     /locus_tag="ACHKV2_001245"
     CDS             complement(32468..32980)
                     /locus_tag="ACHKV2_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939167.1"
                     /translation="MGITSGWVGSSAKSETGEQWMGAAGTKLGLDKPFMMSQMVGRAM
                     GCKIETAYYKWNSSDQVENWGAVGADWPLEEKSKGTITNAENCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLSGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVTQDLKIS"
     gene            complement(33067..35304)
                     /locus_tag="ACHKV2_001246"
     CDS             complement(33067..35304)
                     /locus_tag="ACHKV2_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6939168.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLANIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDTGERGPKGDQGDMGPKGEKG
                     DPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGPAGPRGERGETGPQGPRG
                     EPGPAGSAANVADATTAQKGIVQLSSATDSDDEAKAATPKAVKAAMDVANEAKTKAEE
                     AAAGGGVPGPKGDKGDSGPAGPAGPRGVQGPKGDPGPQGPKGDTGAAGAKGEKGATGA
                     TGPQGPKGDTGAAGPAGPQGPKGDTGAAGPAGPQGPKGDTGAAGPAGAQGPKGDKGDP
                     GVAGPAGPAGAPGPKGDKGDPGVAGPAGPEGPQGPKGDTGAPGQGTELLTTANTWTQA
                     QTFNGGINGNLTVNGNGSFNDVQIRSDKRNKRNLVKLDNALDRLEALTGYLYEIQYSA
                     DGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGIIALLVEGFKTLRHEIK
                     ELREK"
     gene            complement(35301..35951)
                     /locus_tag="ACHKV2_001247"
     CDS             complement(35301..35951)
                     /locus_tag="ACHKV2_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO6939169.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTVKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADRALSARKKVFNGGGV"
     gene            complement(35951..36514)
                     /locus_tag="ACHKV2_001248"
     CDS             complement(35951..36514)
                     /locus_tag="ACHKV2_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939170.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            complement(36498..36959)
                     /locus_tag="ACHKV2_001249"
     CDS             complement(36498..36959)
                     /locus_tag="ACHKV2_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939171.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            complement(37009..37398)
                     /locus_tag="ACHKV2_001250"
     CDS             complement(37009..37398)
                     /locus_tag="ACHKV2_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939172.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            complement(37454..38668)
                     /locus_tag="ACHKV2_001251"
     CDS             complement(37454..38668)
                     /locus_tag="ACHKV2_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO6939173.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            complement(38692..39699)
                     /locus_tag="ACHKV2_001252"
     CDS             complement(38692..39699)
                     /locus_tag="ACHKV2_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939174.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            complement(39857..42001)
                     /locus_tag="ACHKV2_001253"
     CDS             complement(39857..42001)
                     /locus_tag="ACHKV2_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6939175.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            complement(42001..43707)
                     /locus_tag="ACHKV2_001254"
     CDS             complement(42001..43707)
                     /locus_tag="ACHKV2_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6939176.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            complement(43688..44494)
                     /locus_tag="ACHKV2_001255"
     CDS             complement(43688..44494)
                     /locus_tag="ACHKV2_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6939177.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            complement(44787..45338)
                     /locus_tag="ACHKV2_001256"
     CDS             complement(44787..45338)
                     /locus_tag="ACHKV2_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6939178.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            complement(45541..45978)
                     /locus_tag="ACHKV2_001257"
     CDS             complement(45541..45978)
                     /locus_tag="ACHKV2_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6939179.1"
                     /translation="MNRMLSAFTVILLVVCGALSLGLNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(45981..46130)
                     /locus_tag="ACHKV2_001258"
     CDS             complement(45981..46130)
                     /locus_tag="ACHKV2_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939180.1"
                     /translation="MEWWLMISDKLITLAKGLCVIVGISFSLMLVVLFLSINWMALTS
                     AGLVG"
     gene            complement(46130..46699)
                     /locus_tag="ACHKV2_001259"
     CDS             complement(46130..46699)
                     /locus_tag="ACHKV2_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO6939181.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRDKVIQYQEEC
                     DDVLYEYWTKGGVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            complement(46973..47506)
                     /locus_tag="ACHKV2_001260"
     CDS             complement(46973..47506)
                     /locus_tag="ACHKV2_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6939182.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATQVDGKLVVPGMKLSKEKCDQVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(47511..>47582)
                     /locus_tag="ACHKV2_001261"
     CDS             complement(47511..>47582)
                     /locus_tag="ACHKV2_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO6939183.1"
                     /translation="LLTYLTNLYFKIREDRRKAARGE"
BASE COUNT        10913 a        13486 c        11262 g        11921 t
ORIGIN      
        1 taaaacgaag caggatgcgc attaataaag tcacacataa taaaaataga aatcttaacc
       61 agaagtaata gatcatcgtg aaaatcaatc accaaaaaca ccaacacaca aaaatcaata
      121 atttaataat tgttcagggc ttttttctca cgatttttct actatgacat tatcacaaac
      181 ccactagcat gaatacagaa agagaagtaa taagatgaga cgccactgct ttcagatgaa
      241 ccctgtaatt agggtcgaga atataattta cccattcccc taacgataaa ttaaaaaatt
      301 tgcgagttaa tttttctctg gctgaaggct aacgtcgagt tatcgataaa attcacgagt
      361 aattgtaact atatacttta tgaaacacag gaagaaatta ttatttaacg cgatgataaa
      421 aggcgtaaaa aaaaataata atcttcatga aactcacccc gatggcagca ttgtaaattc
      481 gccaataact ggcattcctc ctgcttttta cgggtgtttc cattaacacc agaatgagcg
      541 gattgtttac tacatcaaga acgcatccga agtgtacatc ctggtgcgcc gactgaatcg
      601 cccggagcca tgcggatggc tacgacttaa tacgaatcat taaccctgta aggatacgga
      661 gagcattaca tgagaccaat atccaacctc aatacgagcg cagcaaccta tattccaccg
      721 caacaactcc cgagtagttg tctggaaact ttgacgcttc tggtgcagat gaataattat
      781 atcaaaaggg atgcggacta cagcacgggc atggctgtag ctccactggt tcctgaagag
      841 gttcacttgc tggctgctgc gatgacaacc gaactgcatc gacatcagtt ccaaccggtg
      901 gtaagtgcaa atgatctgca acttcctgaa ccgttcacct tcgatgttgc cggtttcacc
      961 atcaccttca cgaagacgca agagcatgac aacacgggca atctcatgaa aattgcagtc
     1021 agcaagagcg gtgtgtgcac cagtacaaat attactcttg agctgtttca ctccattgtc
     1081 acaaccctga tgtcacgcag tcaatatgga acctttgatc tctggtccgt acggccaata
     1141 ctcacggatg aaagtcaaaa cagggtgcat gaagcagctc gctactcccc ggcccagcaa
     1201 tacggaagag aagaaacaca ctttaccaac aatggcatgc gagaatttgg aaatatgtct
     1261 ccgctggcgt ggcgtaacga tgtggaatta cagcaatcgt gtaatacaag caacatccca
     1321 ccaaatgctg caaatgacga cataaacaac accaggcaga ccatcgagga ccagccagag
     1381 gcggacgagc cacagcaata tgttaaattg actgtagacg acatgcgaaa atgggcggct
     1441 atggaccagc aggcgcgaaa cgctctacag ggagtttcag gatggtgtac gcgcaatcat
     1501 tttaatatca aaaatgccag aaattacctg accgatcacg gcctgaacta cgcgggacag
     1561 gtaaaggtaa acaggccgca tgaatatgcc aaattcaccc ttgaacatat ccgccagtgg
     1621 gcagccttaa acaagcatgt gcgaaaaccg gttggttatt tagaaaagtg gtgtaaggaa
     1681 agaaacctcg cgccaactac tgccaggaat tacctgaaga acgacgggct taccgccctg
     1741 ggtgagttga agctgacagg gccgcagaaa tgggtcacgt tcaccttcgg agacatcgta
     1801 caatgggcga atatgaccca ggaggagcgt aacagcgcag ggggagccga aaagtggagt
     1861 aaaaaacgcg gcttccagtg gtctactgcc agatcctacc tgaaatcgag cggagtgacc
     1921 aagcaaggtg cgagaaaact ggcgtggtta aaaaacagcg ggaatatgtc gaatccattc
     1981 tatctcgccc aaccaaccag ccgcaggaca taagctgaag gtaccgatag cggcaatttt
     2041 gttcatctgt gccgggtggg caaatagcaa ttgcccccga acactccctg ccaggcaact
     2101 tataccgcat ggcagggaac attcgggtta aaaagcaaaa gataactaat tactcttcag
     2161 cttagccttc ctgcaaccgt gacggcggag ccgaatgata gtgcgcatcg gcctcggcaa
     2221 agcgtttttg catcgccgct gacggtgctt tacccagcaa actaaacact acaatcccaa
     2281 tactgccaaa gataaagccc ggaataattt cgtacagtcc cagccagccg aactgtttcc
     2341 agacgatcac cgtcagcgca ccgatgatca tccccgccag cgcaccgttg cgcgtcatgc
     2401 gtgaccacat caccgaaaac agcaccaccg gaccaaacgc cgcgccaaag cctgcccacg
     2461 cgtagctcac taagcccagc acgcggtttt ccgggttcgc tgccaacgca atcgccacca
     2521 gcgccaccac cagcaccatc acacgcccta cccacaccag ttctttctgg ctggcatgtt
     2581 tacgcagaaa cgctttgtac aaatcttcgg taatcgcact ggaacatacc agcagctggc
     2641 aacttaacgt tgacattacc gccgccagaa tcgccgacag cagaatcccg gcaatccacg
     2701 ggttaaacag aatttgcgcc agttcgataa acacgcgctc ggcgttctgg tttaccgcac
     2761 cagctaccga cgggtgctcg ttaaagtaag cgataccaaa gaagccgaca gccaccgccc
     2821 ctgccaggca gaggatcatc caggtcatac tgatacgacg cgcatggacg atgctgtggt
     2881 gagaatccgc cgccataaaa cgcgccagga tgtgcggttg cccgaagtaa cccagccccc
     2941 accccatcag cgagataatg gcgacaaagt tcagcccttt gagcatatcc acgttttcga
     3001 tgctcttttg tttgatcact tccagcgagt caccaaagcc accgacactg ataatgacga
     3061 taaccggcgt caggatcagg gcaaaaatca tcaggctggc ctgtacagtg tcagtccagc
     3121 tcaccgcgag gaaaccgcca ataaaggtgt aaaggatcgt cgccgcagct cccgcccaca
     3181 gagccgtttc gtagctcatg ccaaaggtac tttcaaacag acgcgcgcct gccacaatgc
     3241 ccgaagcgca ataaatggtg aagaacagca aaatcaccag cgcggagata atgcgcaaaa
     3301 tgcggctttt atcttcaaaa cgcccggtga aataatccgg taatgttaag gcgttattgt
     3361 tgtattcggt atgcacacgc aaccgcccgg ccaccagctt ccagttaatc cacgcgccta
     3421 atgtcaggcc aatggcgatc caactttcgg aaatcccgga aagaaaaacc gcgcccggca
     3481 accccattaa cagccagccg ctcatatccg atgcacccgc cgataatgcc gtcacgaatg
     3541 gcccaaggct acgtccgccc agaatatagt cgtcaaagtt tttcgttgat cgccaggcga
     3601 taaacccaat caatatcatg ccaaagatat agacacaaaa tgtcaccaac atcggtgtgc
     3661 taatagccat ctaaagtctc caaaaaatta ttatcggcaa tgtcgaaact tgccgttata
     3721 tctgccaccg gaacggggta acagagttta tgttttacca gggcgaccgt atcctgccgg
     3781 aagcgctggt tattcacaat cgatttaaca caccatttac attaaatttt agtgctcagc
     3841 gacactattt ttcatcaggt tgcactctct cacatttttt gcggttgcac ctttcaaaaa
     3901 tgttaactgc cgcagagaaa aagtctgagt tattttttta atccctgtca tatcgatttc
     3961 ttttattaac atttcattca tttttaagct tgctacgcag gtcacattta acatggttgc
     4021 acaaagttgc aacatcatgg atatttcacg ataacgttaa gttgcacctt tcagaacaac
     4081 aggagtaatg gcatgggaac caccaccatg ggggttaagc tggacgacgc gacgcgtgag
     4141 cgtattaagt ctgccgcgac acgtatcgat cgcacgccac actggttaat taagcaggcg
     4201 attttttctt atctcgaaca actggaaaac agcgatactc tgccagagct acctgcgctg
     4261 ctttctggcg ctgccaatga gagcgatgaa gcaccgactc cggcagagga accacaccag
     4321 ccattcctcg actttgccga gcaaattttg ccccagtcgg tttcccgcgc tgcgatcacc
     4381 gcggcctatc gccgcccgga aaccgaagcg gtttctatgc tgctggaaca agcccgcctg
     4441 ccgcaaccag ttgctgaaca ggcacacaaa ctggcgtatc aactggccga taaactgcgt
     4501 aatcaaaaaa atgccagtgg ccgcgcaggt atggtccagg ggttattgca ggagttttcg
     4561 ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag aagcgttgtt gcgtattccc
     4621 gacaaagcca cccgtgatgc gttaattcgc gacaaaatca gcaacggtaa ctggcagtca
     4681 cacattggtc gtagcccgtc actgtttgtt aatgccgcta cctgggggct gttgtttact
     4741 ggcaaactgg tttccaccca taacgaagcc agcctctccc gctcgctgaa ccgcattatc
     4801 ggtaagagcg gtgaaccgct gatccgcaaa ggtgtggata tggcaatgcg cctgatgggc
     4861 gagcagttcg tcacgggcga aaccatcgcg gaagcgttag ccaatgcccg caagctggaa
     4921 gagaaaggtt tccgttactc ttacgatatg ctcggcgaag ctgcactgac tgccgcagac
     4981 gcacaggcgt acatggtttc ctatcagcag gcgattcacg ccattggtaa agcgtctaac
     5041 ggtcgtggca tctatgaagg tccgggcatt tcaatcaagc tgtcggcgct gcatccgcgt
     5101 tacagccgcg cccagtatga ccgggtaatg gaagagcttt acccgcgtct gaaatcactc
     5161 accctgctgg cgcgtcagta cgatattggt atcaacattg acgccgaaga ggccgatcgt
     5221 ctggagatct ccctcgatct gctggaaaaa ctctgtttcg agccagagct ggcaggctgg
     5281 aacggcatcg gttttgttat tcaggcttat caaaaacgct gcccgctggt gattgattac
     5341 ctgattgatc tcgccacccg cagccgtcgc cgtcttatga ttcgcctggt gaaaggcgcg
     5401 tactgggata gtgaaatcaa gcgcgcgcag atggacggcc ttgaaggtta tccggtttat
     5461 acccgcaagg tatataccga cgtttcttat ctcgcctgtg cgaaaaagct gctggcggtg
     5521 ccgaatctaa tctacccgca gttcgcaacg cacaacgccc acacgctggc ggcgatttat
     5581 caactggcag gtcagaacta ctatccgggt cagtacgagt tccagtgcct gcatggtatg
     5641 ggcgagccgc tgtatgagca ggtcaccggg aaagttgccg acggcaaact taaccgtccg
     5701 tgtcgtattt atgccccggt cggcacacat gaaacgctgc tggcgtatct ggtgcgccgc
     5761 ctgctggaaa acggtgctaa cacctcgttt gttaaccgta ttgccgatac ctctttgcca
     5821 ctggatgaac tggtcgccga tccggtcact gctgtagaaa aactggcaca acaggaaggg
     5881 caaactggat taccgcatcc gaaaattccc ctgccgcgcg atctttacgg tcacgggcgc
     5941 gacaactcgg cagggctgga tctcgctaac gaacaccgtc tggcatccct ctcttctgcc
     6001 ctgctcaata gtgcactgca aaaatggcag gccttgccaa tgctggaaca accggtagcg
     6061 gcaggtgaga tgtcgcccgt tattaacccg gcggaaccga aagatattgt gggctatgta
     6121 cgtgaagcca cgccgcgtga agtagagcag gcgctggaaa gtgcggttaa taacgcgcca
     6181 atctggtttg ccactcctcc ggctgaacgc gcggcgattt tgcatcgcgc tgccgtgctg
     6241 atggaaagcc agatgcagca actgattggt attctggtgc gtgaagccgg aaaaaccttc
     6301 agtaacgcca ttgccgaagt gcgtgaagcg gtcgattttc tccactacta cgccggacag
     6361 gtgcgggatg atttcgctaa cgaaacccat cgtccattag ggcctgtggt atgtatcagc
     6421 ccgtggaact tcccgctggc gattttcacc gggcagatcg ctgccgcact ggcggcaggt
     6481 aacagcgtac tggcaaaacc ggcagaacaa acgccactga ttgccgcgca agggatcgcc
     6541 attttgctgg aagcgggcgt accgccaggc gtggtgcaat tgctgccagg tcggggtgaa
     6601 accgtgggcg cgcaactgac gggtgatgat cgcgtacgcg gggtgatgtt taccggttca
     6661 accgaagtcg ctacgttact gcagcgcaat atcgccagcc gcctcgacgc tcagggtcgc
     6721 cctattccgc tcatcgctga aaccggcggc atgaacgcga tgattgtcga ttcttcagca
     6781 ctgaccgaac aagtcgtaat agatgtgctg gcctcggcgt tcgacagtgc gggtcagcgt
     6841 tgttcggcgc tgcgcgtgct gtgcctgcaa gatgagattg ccgaccacac gttgaaaatg
     6901 ctgcgcggcg caatggccga atgccggatg ggtaatccgg gtcgcctgac caccgatatc
     6961 ggtccggtga ttgatagcga agcgaaagcc aatattgagc gccatattca gaccatgcgt
     7021 agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca gcgaagatgc ccgtgaatgg
     7081 caaagcggca cctttgtcgc cccgacgctg atcgaactgg atgactttgc cgaattgcaa
     7141 aaagaggtct ttggtccggt gctgcatgtg gtgcgttata accgtaatca gctgccggag
     7201 ctgatcgagc agattaacgc ttccggctat ggtctgacgc ttggcgttca tacgcgtatt
     7261 gatgaaacca tcgcccaggt cactggctca gcccatgttg gtaacctgta tgttaaccgt
     7321 aatatggtgg gcgcagtggt tggggtgcag ccattcggcg gcgaagggtt gtccggtacc
     7381 gggccgaaag caggcggtcc gctctatctc taccgtctgc tggcgaatcg cccggaaagt
     7441 gcgctggcgg tgacactcgc gcgtcaggat gcagagtatc cggtcgatgc gcagttgaaa
     7501 gccgcattga ctcagccgct aaatgcactg cgggaatggg cagcaaatcg tccagaattg
     7561 caggcgttat gtacgcaata tggcgagctg gcgcaggcag gaacacaacg attgctgcca
     7621 gggccgacgg gtgagcgcaa cacctggacg ctgctgccgc gtgagcgcgt attgtgtatc
     7681 gcggatgatg aacaggatgc gctgactcag ctcgccgccg tgctggcggt gggcagccag
     7741 gtgctgtggc cagatgacgc gctgcatcgt cagttagtga aggcattgcc atcgactgtc
     7801 agcgagcgta ttcaactggc gaaagcggaa aatattaccg ctcaaccgtt tgatgcggtg
     7861 atcttccacg gtgattcgga tcagcttcgc gcattgtgtg aagcagttgc cgcgcgggat
     7921 ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa gcaatatcct gctggaacgg
     7981 ctgtatatcg agcgttcgct gagtgtgaat accgctgccg ctggcggtaa cgccagctta
     8041 atgacgatcg gttaaacagg tagccggagg atgttacacc ctcctccggc atcattagcg
     8101 tggtcgaatc ccctcaataa taatccgctg cacgttttca accgtttgat tgaaaaatac
     8161 ctcatcgcgc aacgttgcgc ctgtcaccgc ctccacctga ggggcgaaat cggcgtaatg
     8221 ttgagtggaa gcccaaatca taaaaatcaa atgctgcgga tcaatcggcg cgagtttgcc
     8281 gcttttgacc caaccggcaa tcagcgccga tttctcatca attaatgact tcaaatcgcc
     8341 cgtcagttca tccattaaca gcggcgcgcc tgccagcatc tccatacaga acaggcgcga
     8401 agcctgcgga taatcgcgtg agacttccag cttcagacgg atgtactctt tgatcgccgc
     8461 cagcggggcg aaatcttcac gaaacgcttt taacggtgcc agccagatat cgagaatctg
     8521 ccgcagcacg gcaatataca gcgcctcttt tgacggaaag taatacagca gattggtttt
     8581 tgaaacaccc gccaactctg caatttgctc cagccgtgtg ccgtgaaaac cgaattgtga
     8641 aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc gcgcttactg cgcgcgaacg
     8701 tttacccgtt gttttcactg cgccttgcgt catgcgctct cccctcttgt agattggcaa
     8761 caggatagca aaacccccgc ccggcctcga ccttctgcac tctcatcgcg cacagatgca
     8821 tgttttatgt gcaactgttt tgaccgttta gtccactttt taccagacat tttagataac
     8881 gcgatgttaa cacattaata acactccctt tttaaaaact ggcatccgct ttgcaaacaa
     8941 gccaatacgc ggctgaaaag gatatgcaga atgcagcgcc ccgtctgact ttcactttac
     9001 gagacgaaga gaggttgatg atgaaaattg gcgtattcgt acctattggc aacaacggct
     9061 ggctcatttc gacccacgcg ccgcagtaca tgccgacctt tgaactgaat aaagccatcg
     9121 tgcaaaaagc ggagcactac catttcgatt tcgccctgtc gatgatcaaa ctgcgtggct
     9181 ttggcggcaa aactgagttc tgggatcaca accttgagtc gttcaccttg atggcggggc
     9241 tggcggccgt gacctcgcgc attcagattt acgccaccgc cgccacctta acgttacctc
     9301 cggcaatcgt cgcccgtatg gccgcaacca tcgactccat ctctggcggg cgttttggcg
     9361 tcaacctcgt gactggctgg caaaagcccg agtatgagca gatgggtatc tggcctggcg
     9421 atgactattt ctcccgtcgt tacgactatc tcaccgagta tgttcaggtg ctgcgcgacc
     9481 tgtggggcac gggaaaaagc gattttaaag gcgatttttt caccatgaat gattgtcgcg
     9541 tcagtccgca accgagtgtc cccatgaaag tgatctgcgc cgggcaaagc gacgctggca
     9601 tggcgttctc cgctcagtat gccgatttca acttctgttt cggcaaaggc gtaaatacac
     9661 ccacggcttt cgccccgacc gctgcgcgga tgaaacaggc cgcagagcaa accgggcgcg
     9721 acgttggctc ttatgtattg tttatggtga ttgccgatga aaccgacgat gccgctcgcg
     9781 ccaaatggga acactacaaa gcgggcgcgg atgaagaggc gttaagctgg ctaaccgaac
     9841 aaagtcagaa agatacccgc tccggtactg acaccaacgt ccgtcagatg gccgatccca
     9901 cttcggcggt aaacatcaat atggggacgt tagtcggttc ttacgccagt gtcgcgcgca
     9961 tgttagatga agtcgcaagc gtgcctggtg ccgaaggcgt gctgttaacc ttcgacgatt
    10021 ttctgtcggg aatcgaaacc ttcggcgagc gcattcaacc actgatgcag tgccgcgccc
    10081 atctccctgt gctgactcag gaggtggcat gatgacgaca ttaaccgctc gaccggaagc
    10141 cattaccttc gatccgcagc aaagtgcgct gatcgtggtg gatatgcaaa acgcctatgc
    10201 cacgccaggc ggctacttag atctcgccgg gtttgatgtc tcaaccactc gcccggtcat
    10261 tgccaacatt caaaccgccg tgaccgcagc gcgagcggca gggatgttga tcatctggtt
    10321 tcaaaatggc tgggatgaac agtatgtcga ggctggcggc cctggctcac cgaattttca
    10381 taaatcgaac gccctcaaaa ccatgcgtaa acagccgcag ctgcagggga aattgctggc
    10441 gaaaggctcc tgggattatc aactggtgga tgaactggtg ccgcagcctg gcgatattgt
    10501 gctgccgaag ccgcgctaca gcggtttctt caatacgccg ctggacagca ttttgcgcag
    10561 ccgcggaata cgccatctgg ttttcaccag catcgctacc aacgtctgcg tcgaatcgac
    10621 gctacgcgat ggcttttttc tggagtattt cggcgtggtg ctggaagacg caacacacca
    10681 ggcggggccg gaatttgtac agaaagccgc gttgttcaat atcgaaacct tttttggctg
    10741 ggtcagcgac gtcgaaacgt tctgcgacgc gctttctccc acgtcctttg ctcgtatcgc
    10801 ttaaggagtt taacgatgcc aaaatccgta attattcccg ctggcagcag cgcaccgctg
    10861 gcccccttcg ttcccggcac gctggctgat ggcgtggtgt atgtctccgg tacgctggct
    10921 tttgatcaac acaataacgt gctgtttgcc gatgacccaa aggcgcaaac ccgccacgtt
    10981 ctggaaacta tccgcaaggt aatcgagaca gcgggtggca cgatggcgga tgtgaccttc
    11041 aacagcatct ttattaccga ctggaaaaat tacgccgcga ttaacgaaac ctacgccgag
    11101 ttttttccag gtgataaacc ggcgcgattc tgcattcagt gcggactggt aaaacctgac
    11161 gcgctggtgg aaatcgccac aattgcgcat atcgccaagt gaggccgcga tgaaactttc
    11221 actctcacct cccccttatg ctgatgcgcc cgtagtggtg ttgatttcgg gtcttggcgg
    11281 tagcggcagt tactggttac cgcaactggc ggtgctggag caggagtatc aggtagtctg
    11341 ttacgaccag cgcggcaccg gcaataatcc cgacacgctg gcagaagatt acagtatcac
    11401 ccagatggca gcggaactgc atcaggcgct ggtagccgca gggattgagc attacgcagt
    11461 ggtcggccat gcgctcggtg cgctggtggg aatgcagctg gcgctggatc atcccgcgtc
    11521 ggtaactgtg ctggtctgcg ttaacggctg gctacgaata aacgcccata cgcgccgctg
    11581 ttttcaggtt cgcgaacgat tactgtatag cggtggcgcg caggcatggg tggaagcgca
    11641 gccgttgttc ctctatcccg ccgactggat ggcggcccgc gcacctcgcc ttgaggcaga
    11701 agacgcgctg gcactggcgc attttcaggg caaaaataat ttactgcgtc gacttaacgc
    11761 cctcaaacgc gctgacttta gtcaccatgc ggatcgcatc cgctgcccgg tgcaaatcat
    11821 ctgcgccagt gatgatctgc tggtgccatc agcatgttcc agtgaacttc atgccgccct
    11881 gcccgatagc cagaaaatgg tgatgcgcta tggcggacac gcctgcaacg tgaccgatcc
    11941 cgaaacgttt aatgctctgt tactcaacgg gcttgccagc ctgttacatc accgtgaagc
    12001 cgccctgtaa ggaattgcta tgaacgaagc cgttagccca ggtgcgctta gcaccctgtt
    12061 caccgatgcc cgcactcaca acggctggcg ggagacaccc gtcagcgatg agacgttacg
    12121 ggagctttat gccctgatga aatgggggcc gacatcagct aactgttctc cggcacgtat
    12181 cgtgtttatc cgcactgcag agggaaaaga acgtctgcgc ccggcgcttt ccagcggcaa
    12241 tctgcaaaaa accctgaccg cgcccgtcac cgctatcgtc gcatgggaca gtgaatttta
    12301 tgaacggtta ccgcaactgt ttcctcacgg cgatgcccgc agttggttta cctccagccc
    12361 acaacttgcc gaagaaacag cgtttcgcaa cagttccatg caggcggctt atctgatcat
    12421 cgcctgccgt gcgctggggc tggataccgg cccgatgtcg ggttttgatc gtcaacacgt
    12481 agacgacgct ttttttgcag gcagcacgct gaagagcaat ctgctgatta atatcggcta
    12541 tggcgatagc agcaaacttt ttgcgcgcct gccacgtctg tcctttgaag aagcctgcgg
    12601 gctgttgtaa ggagcaaact atgaacattg tcgatcaaca aacttttcgc gatgcgatgt
    12661 cctgcatggg cgcggcggtc aatatcatca ccacggacgg tccagccggg cgcgccgggt
    12721 tcaccgccag cgccgtctgc agcgtgaccg acacgcctcc cacattactg gtgtgcctga
    12781 atcgtggggc gtccgtctgg ccggtattca atgaaaatcg aacgctgtgt gtaaatacac
    12841 ttagcgccgg gcaggagccg ctttcaaacc tttttggcgg caaaacgccc atggaacacc
    12901 gctttgccgc cgcccgctgg cagaatggtg tgaccggatg cccgcaactg gaagaggcgc
    12961 tggtttcgtt tgactgccgt atcagccagg tggtgagcgt cggcacccac gacattttgt
    13021 tttgcgccat cgaagcgatt catcgtcacg ccacccccta cgggctggtg tggttcgatc
    13081 gcagttatca cgcgctgatg cgccctgctt gttaacctcc ttaaggagac agctgatggc
    13141 aatgttcggt cttcctcact ggcagttaaa atcgacctct acagagagcg gcgtggtcgc
    13201 gccggatgaa cgattgccgt ttgctcagac ggcagtaatg ggcgttcaac acgcggtggc
    13261 gatgtttggc gcgacggtgt taatgccgat tctgatgggg ctggatccca atctttccat
    13321 tttaatgtca gggatcggca cgttactgtt tttctttatt accggcgggc gcgtgcccag
    13381 ttatctcggc tccagcgcgg cttttgtcgg cgtggtgatc gccgcaacgg gctttaacgg
    13441 tcagggcatt aacccgaata tcagcattgc gctgggcggg attatcgcct gtgggctggt
    13501 ttataccgtc attggcctgg tggtgatgaa aatcggtacg cgctggattg aacggctaat
    13561 gccgccggtg gtgacgggtg cggtggtgat ggcgattggc ctgaacttag cgcctatcgc
    13621 ggtaaaaagt gtatcggctt caggctttga tagctggatg gcggtaatga cggtgctgtg
    13681 tattgggctg gtggcggtgt ttacccgtgg catgatccag cggttattga ttcttgtagg
    13741 attaattgtc gcctgtctgc tgtatggcgt gatgaccaac gttttagggc tgggaaaagc
    13801 cgtcgatttt actctcgtca gccatgctgc ctggttcggt ctgccacatt tttccactcc
    13861 tgcttttaat ggtcaggcaa tgatgctgat tgcgcccgtg gcggtgattc tggtggcgga
    13921 aaacttaggt catctcaagg cggtcgccgg gatgaccgga cgcaatatgg acccgtatat
    13981 ggggcgagca ttcgtaggcg atggtctggc aacgatgctt tctggttctg tcggcagtag
    14041 cggcgtcacc acctatgctg aaaatatcgg tgtgatggcc gtgacaaaag tctactcgac
    14101 gctggtgttt gttgctgctg ccgtcatcgc catgctgttg ggcttttcac cgaaatttgg
    14161 cgcgctgatt cataccattc cggccgcggt gattggtggt gcatcaattg ttgtgttcgg
    14221 gctgattgcc gtcgcaggcg caaggatatg ggtacaaaac cgtgtcgatt taagccagaa
    14281 cggtaatttg attatggtcg cagtaacctt agtgctgggc gcgggtgatt ttgctctcac
    14341 gctgggcggt tttacgttgg gaggaatcgg tacagcaacc tttggcgcga ttttactcaa
    14401 tgcgttatta agcagaaggt tggttgacgt tccaccgcca gaagttgttc atcaggagcc
    14461 ataaacagaa aaccgccggg gttaccggcg gtgatggctt tttgacgagc ttaagaaaaa
    14521 aatgcattcg tcattgatta gcccggctag ttgtcgcttt tgccgtgact gctcttgcca
    14581 ccttttttac ctgcttctga tgctctttcg cggtcttctg caaaattgcc ggaaccgcct
    14641 cgatggtttg ccattactga cctccgtctg ggtatagaca tcatattgct agaattaacg
    14701 cggcgtatgg cggctgccat attttcgcta cgtgggacta agcttagtga actgacgtgt
    14761 ttctacccgc gtttggtaat atcctgcaac agcttcttag cgagaataga tatcatctcc
    14821 aataattatc cataagccgc tcttatgatt aaaaaaacgc gatgagataa aaatgtgtca
    14881 ttttgcgaca aaattgcgtg cttgtgaaag ttgttataaa tcaaataagt ggttgtgaaa
    14941 tttgcactct gaaaaggacg tcttatcttt aaataagtgg tagcgaatcg ctacggaata
    15001 gagataacac gaggagtggt tagaaatggc taaagttctg gtgctttatt attccatgta
    15061 cggacatatt gaaaccatga agcattaaaa acacagaatt accgaagatc gtccaagagc
    15121 atatactttt caatatgtta gacctcattt tgaccataaa gaaatcgatt ttacaccgtt
    15181 ctttatacgt tcttttttgc cccaaatatg ccccaaacaa ccacactttg ccccaatagc
    15241 gccccaaaga tttaagggga tttaagtata tatttcagat aattaacgca agtttacgat
    15301 gtgaatttac gttaattttc tgggtagggt atttaccgtc attcgttcat caatatttct
    15361 gcatattgac aaatacgcct gagctatcgc gggagaaatg caatgaaagc agcaaaaggc
    15421 gatgttaatc accctgctca tctctgtatt accgtcattg tgacggcact gataacgaag
    15481 aaagacctct gcaagggagg aatccctcgc cacctctgat gtgtcaggta tcctcaccct
    15541 ccccaatcac attagccccg cacttccgta cggggccatt tctgttaatc cactcccact
    15601 tttttgttgt actcgcggtt aaaccgatcc acggcaattt tcatatcccg ctctaccgat
    15661 ttcaccatcg ccgactgttg cgccagactg agagtactgt cggcgtaaat ggcatcacgc
    15721 tgtttacgca aatccttcag tctcttccgg gtatcctgca taagcccgtt catcgacaat
    15781 tttccgttgt tctcgtcaat gaacgccgtt ctttctgcgc cggtcagact cttcagctct
    15841 gcgtgatact gcgcaacctc tgtcatccgg tcgtacatct tctgctggtc aacatacggc
    15901 atcacctcac ctgaaatttt ccccaggaaa ggcacctgct gttccggtat atcaataccg
    15961 ttcagcgatt tcaccgccgc atccgtggtt ttggaaatga agcgccctgt accaccagag
    16021 atatagtcca cccagtattt cagcgattcc ggtgtgatat ccaccgcgcc tgaacggtac
    16081 tggctgccac ctgagaacgc attcagccag gatgcaaacg ccttgtacgc ttctggcgtt
    16141 gaacgtcttc ccagctggct gtcaggtttt ggtgtaccaa acggcatgtt ctcctggtaa
    16201 atctgcgccc ccatgaagtt ttcattcatg gcaaggttcg caaacggacg cagaatggtc
    16261 ggcgctgcat ttttcagcaa tgccccggac agtgtttccg acgtctcact gccaatcggg
    16321 ctgaatgcac caagcacacc accaacaaca ttaccggcag cacgggacgc cgtcaggtca
    16381 cccgccgcca caccttcagc ggtatgcccg agcaggaaga aaacgttgta cccgtaaggc
    16441 agaggaatac tccagtactc tccggccttg cccccgaaca ccgatttcat aatgacgagg
    16501 ttacgctctt tcacatgaga cggcaccttg tcataccagt taaccccgtc atcatcctcc
    16561 cccgcaacac tgcggttaag cgagccaagc agataacccg ctcccacagc tgcaagcgcg
    16621 attttctgcg gtacattgag attcttccag cgaaggcgct ccagtaacgg cccgtcgcca
    16681 ttaagatgtc cgagcgttct caccaggttt gcggtcccct gaatgctggc gttggcgaac
    16741 atgtacagcg agttcatcag cgctccctgc tcaccacgac ggttaaagtt caccgtcatg
    16801 tttttggcaa gagacgccgc ctgctggcgt gacaaaccgg catcacgggc gtgcttatag
    16861 gcagaaagac gcagagcgtt ttcaaccgca ccgttggcat cctcgacaag gttaaggaac
    16921 gaattccatg caccgatact ctggcctttc catcccccct tcgccagtga gacaaggcga
    16981 tccatttcct tctgctggcc ttcaaggtca cccatgttaa accagccggt tttccctccg
    17041 tcctcaacaa actctttcca caccttctgc cactgtgcac cttttcccgt gagggtttta
    17101 ccacgcagac tggcgtatac ggctgacatg gcagagcggc tgtctttcac cacagccagg
    17161 gcggataagt tatccagccc tttcagttta ccgtcgctcc ttccctgctc cgccttcagg
    17221 ttcatcaccg ccgtctgcac gtcacggatg aagttactga ccaggaattc cgggttatac
    17281 gacgtgttca ccgttgccag gaagcggtta actttcccca gcgtacgaac aaaggcattg
    17341 ctggtttccg gtcccatact cttcatcgca cgcatcaggc gcggatcatg gagtttgatg
    17401 tagtacgttt tgccgttctt tttggtggtg aagtaccggt ctgccatcat tgccatcggt
    17461 acagggcgtt cgacaacttc gcgaatggtt tcaccagttt cctggtcctt gcgctctgca
    17521 atcgtccgca tggtatccgg tctgtcatcg gtgaatacct gccagtaatc cttgtcggga
    17581 ttatcctgca ccagtttcag gaaggcgtta cccacttcgt ttttgcgatg gcggatcagc
    17641 gattcgctca ggtcctgtat cgcctgagtg gacggagact gtgcccggga tgcacgcccc
    17701 atggcctgcc tgctttcacg tccgccgatg gtgaagccct tacctgtacg gggcagtgac
    17761 accacaccgt caacatcctg acctttcagg ggaacgtagt aacggtaggc gttctgccag
    17821 gcatccacca caccgttctc ttcaagtccg gcctcacgga taagctcacg gcgacgggcc
    17881 agcatatcgt caataatccc tgccagacgg tcatactgtg cctgtttgcc actgttacgt
    17941 acacgctgca tgatttccgc cgcttccgcg ttggtcatcc ccgaaccgcc gtccggcatt
    18001 ttcgggttga ttttcgcgat atgcgcgtta cgttccggcg cgtgacgggc gtagaggtac
    18061 tcatccagat cggcctgcgc aattttgtag tccgccagta atttagccag tggctgaacg
    18121 tagcgctcct tcatcacgtt caggtcgttt tccgccttcc cgtggaagag ttcttccgcc
    18181 atataagcgt tgttactgtc gtccactttg ccgccagttt tacggatatt ctcctgaaca
    18241 gctttcagca cctggaattt atcctgcatc tggcgcacaa aacgcgatgc aattgtctct
    18301 tccggtgtca gactgctggt acgggagtaa tacggcccct tgcgaatatc ttcaggatag
    18361 agtattttat ccgcagagcc gcgataagaa tgttcccctt tgggcaattg gagccctctg
    18421 tgcagaaggt tatcgcggct taatttttct ctccggtaca acgttaaccc ggtcatttcc
    18481 atactcttca gtttttttcc attttctgta ccgtaaacag aagctacctt gttgatttcc
    18541 agacgcgacc ggacagcctt catgtgtacc gctgacacca ccggatcacc atttttatcc
    18601 acggcatcaa gcagcatcac aaccgcattt ctttctgtcg ctgagcggta aattgcatcc
    18661 ggatcgtgca tcagttccgg tagtctctcg ataacatcca tcggcaccac atgtttcaca
    18721 ccattggtgg ccttccgcac agtatcgcgg gaaataacca gcggcaaatc cggtgcacca
    18781 aggtgacgca ataccggcgg cgtacgcccg atgtttaccg ttaaatctgt ggtgcgcaga
    18841 gatttcatca ttctggcaag gtcatcacga taacgctcgc cctcaccttc cggcactttg
    18901 aacggatcac gtttaccact acgggagtac tgagacgatg cgcccgcgcc atcctcacgc
    18961 ggcgtgtaac cttcccgcac acgctggcct aacgtacgga tggtctcgcg aacaagtctg
    19021 atatcgttca gttccgtcgg cttcagtaac cccgtacgac gcagtacccc tttgaccagg
    19081 gcaacaacac gctcccatgc cgccacgaat ttattcggct gtttctccgc catatgtgcc
    19141 agaaattcac ccgcctgtac ttccggtgat tccttaccat aggaagcatc aaccttacgc
    19201 caggcttcac ggatggtgac gttatcactg tcgcgggttt tcagcacggt tttgataatc
    19261 gtctgatatt ccgctggtgt gacaacatgc tccatggcat ggtgaatgat ctcgtgacgc
    19321 agcttctcgc gaacggtccg cccgtcaggg atgttatccg ccaccaggac aatttctcgt
    19381 ttatccggac gatagaatgc gtgcaccctg ccgtaaccat cgaacaattc acccgccagc
    19441 gcttcagcct ctttctgtga cttcaccaca cggaccttca ggtcactgtc cttaatgccg
    19501 ctcatcacgc cacgggcaac cgcttcaacc tgcgggaccg ggctgccttt ggcttccgca
    19561 ctacggttaa catccgaaat gagattacct tcaggtgtgc gggtaacgcc cttacgggaa
    19621 taaaacgcaa cgcccttgtc cgtctcacgg gttttcaggg tgcggaacag gtgatcgaat
    19681 gcctcacgaa taccgccatc cagttccgca ttcgtcggat aagcccaggt gttatctgtg
    19741 ttgtgctcag gtgccttacg gatattgacc agataatcat tctccacgcc agccatacgc
    19801 gccttatcct gaacataacg ctcaaaggca cgtgccgcca tttcaacatc cgttgaccag
    19861 tacggttttg agcgcacctc atcgagaagc actgaacgac gcggcatgtc actgttttta
    19921 atggcctgaa tcacaccttt aaaagcatcg taaacctcct gacgtaccgg atattcagca
    19981 tcaacatacc tgccgtcttt aaatatgcgc ctgacacgct gtgcttccgt cataaagtcg
    20041 ccacctgacg taattttccc gtcattggaa acgtcataac gaccaaaata attatccaga
    20101 gaatggaacc attcgtgcgc cagcgcaccc ggtccgttac cttttgtcag gttgattgcc
    20161 acctcacccg gctcatagtg tgctgccgcc ttacccttac cacgggcacc aaatgccagg
    20221 ccaagacgac cgttcaggga aagcgctttt gtcggtacat tcaatacttc cgcaaggtca
    20281 tgcagcgagt cataagcccg gttcaaatca gcctgacgac gcggaccttc cacgtagtta
    20341 ccaaactgca caccacggaa accaaacgca tcactgaact gctccggtga aacatcccct
    20401 ttacggcgtt ctggtccggt acggtcgcgg ttggtggcgt tacgctgctc ctcacgcgaa
    20461 atctcccgca tctccttcac atgacgaaca agctcatcac gatgtgaatc aatgtactta
    20521 cgcgcatcac tggctgactt aaagccacct ctcacccgca ttttgttttt gccataagcg
    20581 ataaaaatat cgccactgcg ggtattccgg taaacgtcaa agcggatttt gtcatccggt
    20641 gacggtgccg ttttttcatc tcctttcgcc tgtgactttt cctccagctc tgcaaaccag
    20701 actttcgcct ttgtcagtaa ttcatccctg ctttccgaga aaaagaggtt agtcccctta
    20761 ttgtccttat tgcgcagtga ataaagtttc tgtggcggat cgtaacgctt ccctcctgcc
    20821 gcctgataca cacccgatac cacccgataa gcagaagcct tgtccatctg tgagggtggc
    20881 agagtgcgta acagttgcca ggtatccgcg taacgggagg gcattctgcc ttccatccat
    20941 tctgcgaggc gtttcgcgct gaccgttccg ttaagcattt ccgatacact gtgtcgtact
    21001 ttttttacgc tctcccccca gccagccgta ttgtgtttcg tcttcgccgg aatatcgcta
    21061 cgatacagcg ctatcattgc cagggtgtca gcatcagccc cttcgctcgc cagttttgcg
    21121 tagtccggtt tcgggaacag tttgctcagc ggctgcgtgg cataatccct gtcttccagc
    21181 gttttaccca gtgtttcagc aagctgtgca taacggtgtt tggctgcccc tttaatttcc
    21241 tcgccaaagt cttcaatttt tttgcccctg acttcacctc cgcgcacagt agcttccctg
    21301 tcagtagaga cagttgtttc agattcaggt gcgattgtct caggctgcgg gatgtcacga
    21361 tttcctccgg cctgtcgacg tacttctgcc tgctgtgcga gctcaggcaa tgagttacga
    21421 acagttcggg gaagctgatc aggctgaggc atacggacat tgtgtgtaat gtccggagcc
    21481 ggtagccctt cacgtaccgg tgctgtcgcg ttctgcgcat ccggtgacgg cagaccacga
    21541 cgaaccattt caccctcgag tgtttccccc tggcgaccag cggcgttttc cggtgccgga
    21601 gcttgtcctt tctggaaact ctggccctta acctcaccgg ttgtggtaaa acgaccacca
    21661 cgtccggcct gattttcatc cggcgtacgc gccacttctc ccggtaacgg atatccctgt
    21721 ccgggatgaa tatcgccggg agcgggcagg cgcggacgct cagttaactc ctgtgctgtc
    21781 ggaccggcat caccttcagc catctgtgaa cgtacaagct cctccgccgt cggcgcgttg
    21841 ccacgggcaa ggcgggcctg tacctccgta tcatcccccg tgaaaccacg gaatcgcggg
    21901 tcacgcatga acgcgggctg ctccatcggg tcagcatcag caatacgctc tctgtcagcc
    21961 agggtgttaa tggtttccgt tgccacatct tcagagaacg caggattatc catttcaacg
    22021 gcatactgat tctcgccttt cttcactacg gacggtttca ggccggtggc ggcggcatta
    22081 cggaaaacat cactccccat ggcgcttttc tcatccgtga aataacggtt gtccgggcgc
    22141 accttctcaa tgcgttccgc tggtctggac gcaccattcc cgttaatttc aatgcggact
    22201 tcattcgggt cccggaaccg caccgtcggg taaatgctcc cgttaccatc ctccgcattt
    22261 tcaggctgtg actgtgcttc cggcgtcact tcctgttgtg ggcgaatgac ttctcgtatt
    22321 gcctgaaact gttgttcttc agtctcggtg cgcccctctt tctggctgag tctgcggtat
    22381 tcctcaagca gttcagaacg cggcttcgcc ttcagctcat tcatcacagc ctgtcgcttc
    22441 gtctgttcgt ccagttcatt caacaactgg ctggcagctt cccggcgatg agctgcggat
    22501 gcgtcaccct ctgttgccat atccgcatca gcatactgct ccagaagctg ctcgcgattc
    22561 atgccctgca tggattcacg ctgctgcgcc accggatcaa cagattctgg ttgcggggct
    22621 gcgtcgtcct gctgcacggt ttcagcatca cgcatggcgg tttcttctgc ggcctgacgt
    22681 ctgccacgat atccggcaac cgcaccgaat ggagccccca tcgcagcacc aagggcagca
    22741 ccttcgatcg ttgcgtcagc cacgccctcc catggcgata catccattcc ggcggtctca
    22801 cgcaatgccg tgttttcctg atagcgtgaa tagccgccct gcgccgcatt aatcgccccc
    22861 tgtgcagtgg catttctgac aatgccgctt ttaacggtct tcgctgtgcc tcgtgtcacc
    22921 agattaaaca gttgtgcgtc acccagtttt gccgccatgg cattcacagc cagcaattca
    22981 ggatcggttg ccagctgcgc gcgcacctca tcggcaacac gctcttttgc cagatccatt
    23041 ttctggcgat cagtaagctg tgcgtgctgc gggtcggcgt caatggacaa aaacgtctgc
    23101 tgaaatttcg gtgactgcgc cagctcagag taatcagcat taagaacagc atctgctgcc
    23161 gccattgcac tctgcccctg tgcacttaca gtggaatggg tgatcaggcc cgcctggaat
    23221 aaatccggca tttttttatc gacagcttct gctgccagtg ccgtggctct ttccggctgc
    23281 atccctgccg cgatgtattt tttctccagc ccggcggtca gcatttttcg cagtgtgaca
    23341 tcacccacct ttctagccac accgccagca accatatcag gtacaagcgc accaatcagg
    23401 tttacaccct tcgccaccca gaccgcagaa tcattatagc cttcggtcat cggcgtattc
    23461 agcgcacgaa ctgcacccgg cgacatctta ccggtcagcc attcatcagt ggctttggca
    23521 ccatcactga cagccttacc ggttgccttc agacctttac cgacagtatc agtaaccgcg
    23581 tttttgccat caggcagggt atcgatgacc tcatcagccc ccctgctgcc accggcaaaa
    23641 atatcctgca cagttgcgac acccggcagc cccatacggc taaactcatt taaaatacgc
    23701 gcccctgttt ttaccgggct ctgaatcatc gcatcaccga gtccacgggc catttcccct
    23761 gttccccgga cggactgggc gaaaccttta cccattgttg gcaatacatc gcccaggctg
    23821 aacgacgtac tgttatcctt ccagcgattc ggatcagaga aaaatgcttc atagctgtca
    23881 gtttcgccgg gttgctgaat gttcaggctg ttacgattct ggttaccgag ttgcgcctca
    23941 ggacgctgtt cctctgaata ggccatacag actccataaa aaaacccggc acaatggccg
    24001 ggcatcagga aatggtatta atggagtatc gtgaattact gggcgtaatt ctgtttcagt
    24061 ccacggataa actgagatgc agatgaagca ctttcatctc tggcctgttc ccccctggcc
    24121 ttttgaatac ggagaaaatt cgcatacccg ttctccagca atttctgatt ctgaggttcc
    24181 agcatttcag gctgacgggc cgctacattt ctggcaaatg acagcttatc aggatcatct
    24241 cccgcccagt tgataacctg ttgctgaagt ttttgttgct gaattttctg ttgatgcggt
    24301 tgtgaaccag tcgcagcata atattcatcc accgcagcct gagcattacc gccattcttg
    24361 atcgcgtccg ccgctacgtt gctggccccc ttctgaagct ctttcagaga aagtccctgc
    24421 tgcttcggca taaaataacc ataatttttc gatatctctg caaatttgct gcgatccctg
    24481 acctgagcga tagccttatc aacgggtatc gccagcacgg tttgatcatc agggtgcgca
    24541 ctgccgtatt ctgttacagg tttatgcgcg gtggagccat cgctgtatgt gagatcaagg
    24601 ccaataagta cgtacccttc ctgctgcgct ggtacgatac tgccaatcct ggcctctttt
    24661 atcgtttttt ccccggttga atcaggcatg ccaatacgct gttgtagttc cggggcaaac
    24721 acgccggaaa gcacatcgag attttccggg gtattcagcg aatcgatcgc catgtccggc
    24781 ttatcaccaa agattttttg caggttgatc acggcctgac ccgctttcgc tgcatagcct
    24841 tcgtttgaca taagaattaa tggatgtccc tttggcagct gcccgaacag acgcgttgca
    24901 gcctcctgat cgccagcctc aattgctttt ccaagcgctt ccattagtgg ctggctatgg
    24961 gcaatcatat cgttatactc aagccgtcgc tggttgtatt tctgcaactg gagtcgctgc
    25021 tgctccatac ccagtgacgc attccggtaa ttctggttag cgttaaactc cctttccttc
    25081 agtgcataat tacggtcgtc aactttggct ctgtaatcaa agttccgctg atcaacgttt
    25141 ttattatgtt caaactgaga ctgcgcaaac tcaaaatcgc gctcgttatt tttctgttgc
    25201 cgggcaagct gtgcttcacg cagaccaagc tccttacgac gggtcattgc ctggtcaacg
    25261 gtgctgaatc cggcaagtaa cccctgtgca aatccgctca ttcaccactc cttaaaacaa
    25321 agaaccagca atgccgccaa ttactgctcc agcaacagca ccaacaggac caccaacgga
    25381 tgcgccaata gccgcccccg taccaatgcc cgtaccgata ttctgcttgt tctgcgcttt
    25441 ctgttgcgcc gccatctgtt tgttcgcagc ctcaatttct tcacgtcgtc tgtctgcgtc
    25501 acttattccc tgtaatgcct cacgccgcga ctgatttgca atatccagta aaccgtatcc
    25561 catattgccc ccttacgctg ccaccagttg gccgccaaca ctcagtttct gtcttgccgg
    25621 tgcagaagcc cccgtcagaa tattcatctg gcgatcctgt tcggcttcac ggataccatt
    25681 tttcgcgcca gcaattgcca gggcattacg taaccccagc gtattactgt cgggattatc
    25741 cgggcggttt accccgtatc tcgccatctg gttatcctgc gccatctgcg ctgtacggag
    25801 actggaagtg gcaaggcctc ccacccgtgc aagctgtgca ttcatcagac tgttgttctc
    25861 cccaaggtca gccagccttg ctacacgggg taaatatctg gtgcgccagt cgtcgtattg
    25921 ctggcgggtc agcgctgcgg atgtctgcca gtccccctga gggcgggctg ctccggtata
    25981 accgtatctt gcaagtgttt cgtatttacc gtactccata atcacagtct ccagttctga
    26041 gcctgatgct gaatggcatt agcgccggtg ccaggtgttt tagcaccgcc acttcctccg
    26101 ctaccgccag ccttatgcat cgcatatgca cctgccgcac ccagaccagc gccaacaaga
    26161 gaggctcgcc cctgctgttt cgtaaacgcc gcctgtgcat ccgatttagc ttttgccaga
    26221 ctgctgtctg ccagagagtt aaaactctgt aacgcatccg ccttctgacc agaaccgaga
    26281 gcggcaacat cctgtagccc ggcaacatac ttatctgcct gcgatacctg cccccgtgtg
    26341 gttgtgtcaa tctgcccggt tacctgatca ctctggttcg cattcattac cgcgttaaag
    26401 cgaccactgg acgggtcaac accggactga gcaagattac ccgccagctc ccttcgcgct
    26461 tcaccaaact gtttctgata accaagattt gttgtgccag cgatattgtc gtactgctgc
    26521 tcactgttaa ggtcatcgac cttttccatg aagttatctt cagccgggcg gaggatattt
    26581 ttgtaatcct gccacccttt ccaggccact tcttcctgtg ctatttgcgc tgctgttggt
    26641 tttactttgg tatcaccacc gcctttactt ccacccataa tggccccctg gataacaaaa
    26701 aaccctgccg gagcagggtc agaatgtgaa ttacaatgtt ggtttaaaca caatgatgaa
    26761 aactgtagca gtcagaccgc tatcctgaac accatgaagc catcctcatc atccggcatt
    26821 cgctcaaagc ccagtcgttt tcccagccgg ataaatcccc gccttgccgt atggaactca
    26881 gcccagcgtc cgccagccag atgggttaat gtcttcacct ccggcaggta acgctcaacg
    26941 ctgttactcc ccgtacacac gcccagcaac accagaacat aagggatacc atcagcactg
    27001 agcacagaac gcagcaccaa aaagccatca ggtgcctcaa aacaaaacgc ctgcttttta
    27061 aggcaggcgt ctttaacttc attcataaat tcagggttgc gggaatttct cacaacacgc
    27121 tgcatatacc agagaatttt atcgttcatt ctctcacctg aaacgggtgc catatcggct
    27181 gaacctcagc aaccagttga cggggactct cgtccccgtc gcggttttcc tactgcttac
    27241 actgtaagaa cgccgcaaac tccgctcccc acaaattcag ccggaactca cacagtgaac
    27301 cgtgcaacat ccagatggtg aagataaccg tcatgcaaat tgtgacggtg ataagcgatt
    27361 tttgcgacat agcgcttgac tccttttaca gagaggcgct aaccttctac ttgcttaagg
    27421 tacgaagtgt tagggcctcg ggttaacgtt aagttgactc ggggcctttc cacatcaggc
    27481 cttcaggttc accctccagc catcagccga aaggcatcca cgcataattt acggtttttg
    27541 ccctgtacgg gcaataaaaa acccgccatc acagcgggta gtaaacggtt accggataca
    27601 ggtcagaatt tcaggccaat gccagcagta acgccactcg tgcgccagtc gccagagccg
    27661 gaaccttcgt aagccacatc cagtgtaaca ctctcattca ggttaaactg tgcacctgca
    27721 gcccacgcaa gggaggtttt tttcgtgctg ttgctttcag aaaatccgcc actactgtta
    27781 atattgtcct taattttcag gtcagcgcta actttagcaa cgcccatccc tgccatcgca
    27841 tataaactca tgtactgatt aacccgccat gaaggccccg ctaacagact ccagtaattc
    27901 gccctgatat ccgttctggc agaagctgcc ggattcttaa tcttcctggt atgatcggct
    27961 gactgcacat caataaatgt ctgtgagtta gtgagagaac gcgtccatgt aaaagaggtg
    28021 ataacgccaa aatcatccgt tatctcgtag cgatacctga tattaatgcc ctgtggattt
    28081 ttatccttgc cttcatatcc actgacccga ccatctgtat attcgcccaa tgaagaaaag
    28141 tagtttctgt tgacgaaatg actgaaagtc tccctgttat gagcagtcgc atcctttaca
    28201 aaatccttca gtcccggaaa ctgaaagtgc gcatacccca gagaaattgt atgctctcct
    28261 tctgctgcat gtgcagataa atttacacag gcaatcccgg agattgcaca cacaaccagt
    28321 gttgctatac tcttcattta agatatcctg tattcagata acacgctaaa ctttttaata
    28381 atacatcttt ttagacattt taaatgaata ttaaagactc gcttcaacta atcatatact
    28441 gatgtattaa taaccaggac cttatctata tacggctttc gaatatccca tgttgcacca
    28501 ccggaataat gctcacagga atatatttta tttcctgtag caccagtgga tgtggtataa
    28561 atcggtcgat cataaggagt tcttttccag ttataatacc cgaccagtgc aggcatgatt
    28621 gcgcatggat atccaaggtc cttttcaaat ttgatatcaa caggtattaa cttcgcgtca
    28681 agcagcatca tttcgccatg ataaatcatc tcaccgtccg ggttatacat ggcgatacca
    28741 tactcagaag gtggagtaac catattcgcg aaagcataaa ccgtcgtaac accagggttc
    28801 gttcccctga caattacatg aagccttagt gcatgatatc catcaatctg ttcatgcgtg
    28861 tacatgacat cggccttctt ctctgttctg ataaagaaaa aacaactttt gcctgacggg
    28921 attgatgttt taaaaaaaga cttttcagtc gccggtatgg tgcctttgtt gatcagacac
    28981 tgcggcgtaa aacctggact tatccatacg ctgccatccg gcttcataat gctcaaaccg
    29041 tacataacac ttatccccag aatgtataaa tataagatcc cataccctgc tcaagattcg
    29101 accacgtcac cgtattgtca ttaatggtta tcttcggtac tttccgatcc gcaaatacat
    29161 tattccaggg aaataaacaa catacagcct gcaatgattt cccgtcgggt ttattagtgt
    29221 acgtctttga gccagactcc gctgtaaatc tgtccaggaa aaatactgga gtcagcacgc
    29281 ccgtaacatt aacgttattt ctgttataaa tggcaaaacc gtattccaat actcacctcc
    29341 tgatcagcgt aatctgccta tgcgaacagc cagtcgtcca ttctgatcat aaacctcaat
    29401 tttatcattg cggatcacca gtcctacatt ctgattagag taacgaattg tcagttgccc
    29461 ttgtgacgta acactgaaaa ggcctccaat attcaggtta ccctgagaat caacctgaaa
    29521 gtttccgttc tgaataacgg cactccggat aactggcgaa gtgatactta ccccggcttt
    29581 tacctcatcc gccacaacct tccgcgacac cagtgtttca atcaccgcgt cataaatcat
    29641 cgctttcggg atcacaacct tgccacctga taccgcaaac ggataggcgg tgttatccgg
    29701 gttgttcggg tcaaagacaa acaactgcga cgcagaaatt gcaacctgac ttacaggcct
    29761 gccttcactg tcttttccgg cgacaatccc gatccccgca gtgataccat caactcccgc
    29821 tttttttgac cacattgcca gaaacgcctc accgccttct ttatccagtt tagtgatgcg
    29881 cttgtcgacc tcattaagcg attcactggt tgacgaatcc agtgtgctaa tgcgggtttc
    29941 aataccacca atcgttcttg tcgtttcttc cctgagagtc cccacaactt cggttgtctt
    30001 aattgcagca tcctttacag cctgcccctg cgcgtttttt atttctttac gcagctcgga
    30061 cacaaccggc gactttgcag cctcatcgcg gatctggtca atgatggcct tcacgccgat
    30121 ctgtgtttgt gcctgagtgc ctttttcagc attccatgga cctttcactc ctgccgcgtt
    30181 aacaaaacgt atccagtaaa atcccgacca gccagggtca accggatcgc cgtaaacctg
    30241 ccccggcgtc gtggcaacca gcactgcatc agcaaggtca tcctccgtac cccgccagat
    30301 ttcagtcagt gaatgtccgc gataattagg catatcccat tcaagaagaa ccgagccaaa
    30361 tcctcctgtc gccttaaaat tcagcggttt tgtgggaaaa tcaacagtca ttaaagtact
    30421 gtcaatctca atacccggat tcagtgcata tgaggcacca cccgatgttc gacgccgggc
    30481 gagtttaaga ccaaccagtt cctcacgggt cacaaatgcg tggcgtccgt caccacgctg
    30541 cccggtgcca atttccatgt tctccacaac tgtggataaa tccttccccg cacgccacgg
    30601 ttttctggtc ataccggcat ctccgacatc gatgtactca gggttattcg ttccacctgc
    30661 ccgaatccgg ataccatcac ctgccagttt tgcccggttg ctgccggaag tctcaccaca
    30721 cttcccttaa acgtacccgg cgcaaaatta atcacaggaa catcatcagc cataatggtg
    30781 atccccaccc gctcaggcgc cggagatttc accctgatac aggaaaaaga ggttctttca
    30841 ggtaatgaaa aaatttttga atgccacctt atcgtggagg gcagagagcc cccggcaagc
    30901 actgacattt tgtctcctgt caccacgcgc atcatatctt tcgcgagatc aacccatgcg
    30961 cagtcaaacg gtgtactgag ataacggata tccatgttca ccggactgaa tacaaacaca
    31021 tcctgcttac catccggttt cgtgtaacag gcaatgtact caccacgcca ggaataagcc
    31081 acaatggacg ccgggttaaa ctgactctgc cactgttcag gtgaaataat cttttccgtt
    31141 gccagtgctg tattaccgtt tacatcaaca gataccagac cgtttgtccc ggcatagagt
    31201 acgaatccct ccatcgccac catacttctt cggctcaggc atgcctgcat ggaaggaatt
    31261 ctggagccag aaattgtgga cggtgatacc ccactgaaca gataaggctc cccctttgtc
    31321 gccaccacca gtgacgttcc cagcggacag atagccacaa tatcttctgc cgtcgtgtga
    31381 cgattcactt ccggccatgc atacggcaga tacgcttccg aaaacatcac ttcattaccg
    31441 gcaaacccgg cggcaatacc attagccatc aggcaaaggc ctgtcatatt ctctggcggc
    31501 ggcaggtaat cccatgtcgc cagagaaggt ccaaggtttt tccccggtat tttgtccgtg
    31561 taactgagca cggatgcatc cagttcagcc acaagtaaaa aatccgcctc ccctccacct
    31621 gatgcagagc gataaatccg gcggcgttta atactggcat tctgcaatgg caccggagac
    31681 agcgtcagct gtaccgcagt ccccggagta cggagtgtta cctccagaga cgccggacct
    31741 ggcggacctt cttcaccata atctgagaca aaggtttccg tataaaaccg ggtttcgtca
    31801 tcattcgggt tatcgtcaga aacatcaccg ccctgctgaa cagtacagac aggtgctgtc
    31861 gtcggcgcgg ggatccccag acgatacgat gatgccggat gattcccgtc cccttttgtg
    31921 gcaatggttg catccgtcac tttaggaaaa cgcccgtcag tgtagtaaat acgcccgtgg
    31981 gggtcctgag cgatcggact gcggatcaca tccactacat ccgtccatgc aaaccagaaa
    32041 tcgtcacggt aatgaaaaat ggttttcggc ttaatcgcga atgttttctc agcctctgac
    32101 atctggtgtt caggcgtgat cactccatag cgaaaatgac agttttctgc cagtacagca
    32161 gaatgatctg gcagcataga tgcaataacg cctggcatca tcccgcgcat agttgttata
    32221 tcgatataag gcatggttgt ctggtatccg aaaatttgaa tggcaggtga gtcactgaat
    32281 tgaagtttta aaggcggcat acaattgcgc ctgtgcagaa tacagatttc cagaaatctt
    32341 gaaaaattac atcattatta tcagcatccg tccggaaaaa tttctctggc aacttaaaac
    32401 aatatcctat atatccggcg tctccggtat gtagaacgtg aagtttttaa aattattcat
    32461 ttaccattta actaattttt agatcctggg ttactcccgt ctttttcatt gcctccacga
    32521 aagcggaact aacagaacct gaccagtaat actggaaccc actaacaacg ccctgataaa
    32581 tcatggtttg tgtagcacca cctacgttta cggtgatgtt acctgcttta ccaccggata
    32641 aataaacagc agctgtcggt gtagagttcg tcaaaaagtg agaaagtgta acgacagccc
    32701 ccaccagtct cccagaacca cagttttcag cgtttgtaat tgtccctttg cttttttctt
    32761 ctaatggcca atcagctcca accgcccccc agttttcaac ttgatccgaa gagttccatt
    32821 tatagtaagc ggtttctatt ttacaaccca tagctcgccc caccatttga ctcatcataa
    32881 aaggtttatc caatcctagt ttagtgccag cagcccccat ccattgctca cctgtttcgc
    32941 tcttagccga agaacctacc catcccgatg ttatccccat ttcatatatc cttctgaaat
    33001 tcgtgaggtg agtatcatcc ccacaattta aattcagtag aaaccagaaa ctacaacagc
    33061 tgtcgtttac ttctcccgga gttctttaat ctcatgacga agtgttttaa agccttcgac
    33121 taacaatgca attatgccgt tgtagttaag acgcagacgt ttttcaccag atataacgtc
    33181 tgcgtcttca gttaccagtt caggcaaggc tttttgtgca tcctgagcaa ttaaaccaac
    33241 cgacgtttgc caaccgtcgg cagagtactg tatctcgtaa agataaccag taagtgcctc
    33301 cagacgatct aacgcattat ccagttttac cagatttcgc ttgttgcgtt tatccgagcg
    33361 gatctgaaca tcgttaaatg atccgtttcc gtttaccgtc aaatttccat taataccacc
    33421 attaaaagtt tgtgcctgag tccatgtatt ggcagtagta agcagttctg ttccttgccc
    33481 gggggctcca gtgtctccct tcggtccctg cggcccttcc ggacctgctg gacctgctac
    33541 tcccggatca cctttatcgc ctttcggccc cggcgcacct gccggacctg ctggtccagc
    33601 cacccccgga tcgcctttgt caccttttgg tccctgtgcg cctgccgggc ctgcagctcc
    33661 cgtatcccct ttaggtccct gcggtcctgc cgggcctgcg gctcccgtat cccctttagg
    33721 tccctgcggt cctgccgggc ctgcggctcc cgtatcccct ttaggtccct gtggcccggt
    33781 tgcgccggtg gctcctttct caccctttgc cccagcagcg ccagtatccc cctttggtcc
    33841 ctgggggccg ggatcacctt tcggtccctg aacgcccctc ggcccagccg gacctgctgg
    33901 ccccgagtcc cctttatctc ctttcggacc gggaacacca cctcctgctg cagcctcttc
    33961 tgcctttgtt ttcgcttcat ttgccacatc cattgccgct ttcaccgctt tcggggtggc
    34021 tgccttcgct tcatcatcac tgtccgttgc gctgcttaac tgcacaattc ccttctgtgc
    34081 cgtcgtcgca tcagccacat ttgcagcgct gcctgccgga cctggctctc cacgaggtcc
    34141 ctgaggtccg gtctctcctc gttcgcctct cggacctgca ggacctggtt cacctcgggg
    34201 gccagtctcc ccacgctcac ctcgtgctcc catcggtccc tgtggtccgg cttctcctcg
    34261 ttcaccttta ggaccttgcg ggcctgcagg acctcccgga tcacctttct cgccttttgg
    34321 ccccatatcc ccctggtccc ctttaggccc ccgctctccg gtatccccct tcaggcctgg
    34381 tattccctgc ggtcctcgct ccccctgttc gcccttctca ccacgcggac cagcgggccc
    34441 tacagccccc tgagcaccaa cgtcaccacg ctcacctttc ggccctgcgg gcccttgagg
    34501 gcccactgga cctgtttcgc ctttaggacc gacatccccc ttcggaccag tttctccctg
    34561 aggcccccgg ggcccccgtg cattctcagc catacgtctg gcctcttcag cactgacagt
    34621 ggcagcctct gcccgcttaa ggatctctcc ggcgctctcc tgcgccagcc tggccttttc
    34681 agcatgctgt ctggcttttt ctgcatcagc tccggcggct ttttcagact ctccggcacg
    34741 ggtcgagctt tcctctgcat tccccgctgc tgtgactgca cgggtcgcag cctcagtggc
    34801 atcagtcgct ttttgtccgg cttcagccgc cctgctggtt gccgtctttg cactgtcaga
    34861 tgcactcttc gcactggctg ctgcactttc ttttgactgt gtggcctgag tgttttttgt
    34921 cgccgtgtct tcattcaggc gacgaatatt ggcaaggtca tcagccacat tattctgtat
    34981 ctgccggaaa tctgtcagca gttctccggg tatgctaacc tcaacaagac tgcggcgtaa
    35041 cagcatattg agcgtcaccg tactttcggt cccctcaata cgcacacgtc cgtagacagc
    35101 agtcttccct ttcaccgtca ccgaaaccgc atactccccc ggatccatcg tcattccgta
    35161 atatccacct tcacgggtca ctgccgacgc actggtgccg ctgagcgcat ccggtgaaac
    35221 tgtcagcgcc gtcagggtaa tatttgctcc tgatatcgcc tcaccatcag gagatttcag
    35281 cgtccccgaa acaacaacac tcacactcca cctccgttaa acactttttt acgggcagac
    35341 aatgctctgt ctgccccctg tttgatccca agttgctcaa caaaactctg ataatgctgc
    35401 gcagccagcc ccgattctgc accaccggca gcatccttac tgaaagcacg aaacaacatc
    35461 cagtccacca gtgggttaac ataagcctct tccagtggaa ctggcgtatc atcgtcctgc
    35521 gtcagaacat acactgcctc cggtatccgg cttaccactg catcaatact tatctctttg
    35581 tcagggacag gaaacagcca gaatacgcgc ggggacaggt cgttgctgat aaaacattca
    35641 ggaatgccct tcactgtggg ccactcagga tactgcgcat ccagcacctc ccgggataat
    35701 ggtctgactg cactaccgtc actgaggcat atcacgtcaa gaagttgtat tacaccatcg
    35761 ggcaaaacct gacgggcgcc aggaacacaa ctgattgttt ccaggcttgc gccagcatcc
    35821 ggtctcgcca gaatcactgc cctcacagca tcattgtaat aatcgcacaa ttcctgcagg
    35881 ggccagcgaa ccatcatcgg gtcaaccagt tgtgtattca cacgtccgat gatttctgta
    35941 atcgtcgtca tcagaaaaac ctctgcctgc gtacagggtt gcggtatgaa gagtacgggc
    36001 ttgtcgccag tgtatgacga tatgcccgac ggatcccctc agaaaactgc acagaaaaat
    36061 actgtgcgcg taacggatct gaccatgaaa caccagtctg catgaacaac cgctcaagtg
    36121 cccccgcagc cacttcttca ggccatgtga ggagttcatc cggtatctgg ctgcgtccgg
    36181 ctttcggagc gacggcataa agcacgctca cctcaccggg agaacaggca aatcgcaggg
    36241 agcgtccgga gctgatatcc acatcccgac cgacaaaaag ctcatgatta tcgtcagaga
    36301 tacggatgat atgaacgcac tcctcatcat ctttgtcata cggaagcacg atttcttttc
    36361 ctgctactgg tacaacagta acctcccgac ggcacaccaa cgactggcgg ctgaatgcca
    36421 cggcagccat tgacagagca tccgtcatca taatgttcag tggaccgctg atatgacgac
    36481 ggacatacgg taaaaaatca ctcagttccg ccatgctgtt cagtctccgc aacacgacgg
    36541 cgaaatgcct cacgcacccg gatacggaat gcctcagccg tttctttcgg gtctttgtga
    36601 atatccagct cttctgcctc acacagcgtc gccagccgtg ctgaggtgag cttacttaaa
    36661 tccacctcct gcccgttaac agaaacaaca aaactgttct ccgcttctgc ccgcgcagca
    36721 agcactcttt cctgcgcctg ctgtgcctgc cgcaactgct cattctgttg ttgctttttc
    36781 agaacatcat caagctcttc atgacgaacc cagacatccg gaaaccccag cagttgccag
    36841 gccatcgcac tgtcaacatg caccggctca agacgtggga acaatgtgcg gcttccggta
    36901 atggtgtcct ttttcacggg ttttgggccg atatagacaa cggcaatttt ctcactcata
    36961 taattccccg gataaaaagc ccgcatgacg cgggccggaa ggttttaatc agtatcccac
    37021 cacggtataa cgcagcagaa cattcagggt gccggttgca gcggcagtct taatggtgac
    37081 agtaaccagc tccccgtcac gctgtgtggt gtacggctcc actggcacat atctggcaaa
    37141 ttttgcagaa acagcttcgc tgttatcgat gagagcatgc tcaccggact taatgctgac
    37201 ggttgcagta cccagaccac ccgttgaaac cagttggagt gagttgatac ggatgcccac
    37261 tggcagtgag agaagatgaa taacactgtc cgcttccgca gcattcaccg taaatacgcc
    37321 ttctgccacc gactcattac cgtgcgtacc cgtatagacc cgttcactca gtgacggggc
    37381 aaggatagtc tttgccataa ttaatgactc ctgaaaaagc cgggcgaaaa cccggcatag
    37441 ggaaaggaaa aaatcagagc ttcactgctg tatcaacggc aatcacgccg tgatcctgca
    37501 tcttgccgct cttctcgggg aaacggattt ttttcagacc gttgatccag ctgattgcta
    37561 tctcagtacg gttatccata tccgttttct tctcaaccat gttgaagtga ccgcccgcct
    37621 tctgaccgta agcatttgcc agcgcctgag cccccagtaa catggcgcgg tcaatattgg
    37681 ttgcagcagc gacctctttc gtggttgccg tcaggttatt ctctgatacc agaacctttg
    37741 acccctgata gaaacggatc ggcatacccg catacttacg aaccaggata ttgcgccaca
    37801 tcgcacattc acctttgaac agcggatgat taaaaccttt tgcacggttc acggcacgaa
    37861 ccatcatctg gttccagtcc ttaccggacg tcgaggtgta ccagtcattc cactgacgcg
    37921 gcgtgacgta caggacgtaa tatggatctt ctccgtgaag ttcatcaccg gacagacgaa
    37981 ccggctgtaa cggatgcgcc atttcgtcaa tgaacaggga gagattgtcc accaggccaa
    38041 tagaaaaaat atctgccgct tcaatctgct caaagcttgt cgcatcaccg ccaaaaaagt
    38101 gacggtcatg tgtcggaggc agtacatcgt tgatcatgat ttttttgaat tcagggtgct
    38161 ccgctgtcgg cagaatagtg tcgtcagcaa caaaatcacc acgagctcca gcaagatgca
    38221 ctatcgcaca ctggtcctgc aggtcattaa agtacgtccc cagaagcgtt ctggctgagg
    38281 atgccaagtt aaacttcgtg cgctgctgac tcatacgtcc gcctgcatcc accaggtgac
    38341 gtccctgatt gattttcagg gagaagtcag catggctgag atcctcacca cgaccttcaa
    38401 cacgctcatc tcccatcgtc gggcgttttg agagtttgtg catgatgctg aaggtcactt
    38461 catcaccggc ctgtttgtta aggtctgtga tacggacaac cggcgcaccc gcgctggtct
    38521 gcttcgtgct tttcttgtcc ggcgaaaccg cttttggcgc ttcctgctgt tcagtgagga
    38581 tattgaccat cgagcggttg cggttggcag cggtaaaaag cgccacctga tacagcttat
    38641 tcgcctgggc tgatgttaca gtcgtcatta cttcagttct ccttcagtaa gttacccgag
    38701 cttctccaga agcgcgtcta tttcagcatt cgtcataccg cgcataatcg cctctgcctc
    38761 tgaatgagaa gcgccaagta accgttcaaa attatcaccg gttccgacgg aagccgtggt
    38821 gcctaaatct gacggggaag caggtactgc ctgctcctgt tcagcggtct tcactttctc
    38881 ttccgccgtt ttccggatat ccgttttgtc tgccttgttg tcagcagacg actcactgac
    38941 ttcaccgaaa gcaacctgcg tacgacgggc cacttcagcg aaacgttcag tgagcgtttt
    39001 gtctttccat gcggggtcat tctggagctt cccgtcgatg gatacagcaa ccgagaagcg
    39061 atctggatcg gactcctgcc acgttttcag caccggcacg gcattcatcg catcaagaac
    39121 cggtgataaa tcctcaccac cattaccttc tgcctgctgt gctgattgct gaacacggga
    39181 ctggagatag ttatttttac ggatgagcga agccaccgcg tcaccaattt ccggatacat
    39241 ctccctgata cgggcaatct gctcatcaga aattttttcg ttttccggta acggtgtggg
    39301 cttcataccg gcctggtgga tctgagacgt cagcagttcc accctgcgtt tttcttcagc
    39361 tatctgccca cgaagaagtg cggcttcctg ttcggcccgt tgcttaccgg aacgttcagc
    39421 ctcaaggact tcatagggaa tgacgtgttt accgtcgcgg gtgagcaccc ccttcgcttc
    39481 cggctccttc acgtcctgcg tctgctccac actggcatcc ggcgtcggtg ccacattgtt
    39541 atcgcccgtc tgagtctgtg cttcctcatc cgcatgtttt tccgtggtat cttccgtcac
    39601 gacgtcctgt gcgtgactgt caatatccac atccccaagt ccttccagca ttttttccag
    39661 ttgttccggg gtttcttcac ccgtaaattc aaaatccata aataactccg catggtctgt
    39721 ttatcggaca gatccgaatg gttgagtaaa taaggcttat cgctgccccc gcgaataagc
    39781 gcaccgctcc cggaacgctt acctccggaa acaaaaaacc ccgtacgatg acggggttca
    39841 gttgaagcca gagttttcag agcgacattt cattcatccg ctgttgtaac gtatacagca
    39901 tctgttgctg aagaacgtcc tgctcctgtt ccatattctg tacgccggta atgatttctg
    39961 ccgtatgtgc ctggttaagc gcatccacat aacgctgccc ctgtgtcagg gcgacttccc
    40021 gctgtgcact ggcattatcc cgttgtgcag ctgcatgtgc cctggcggcg tcagcttcca
    40081 gttttgccac tctgccagcc atctcgcgca tctggagttc tgcctgttgt tgctgaagtg
    40141 cctgttgttg tgccgctact tcctgttctt ccggcgtcat ttcatccggt gattttggcg
    40201 tccccagcgc agcacgaata cgctcaacaa actcctgttt ctgcggcaca tccagaagat
    40261 taacccacag gtcgagcaca acagcctgca cctgaggcgg cagcccctga ataacctctg
    40321 acattctctg tgcaagctgt gccttaaacg ccggtgtctg ctgaacaggc gccagcgcaa
    40381 tatgtgtatt taaccttgaa atatcattgg tcagttcacc attatcacct tcagcattga
    40441 ggacaatggt ctggcgacgc tggcgatcat cgcgattaat caccactgca tgattacggc
    40501 gttttttcag gtcatcgaga agataagcca gcaacagtct tcccacctgc tggcaggcaa
    40561 actggtagtt atcgttgatt tccgcaaggg ttgtggcccc ctgctccacc aggttactga
    40621 tagccacgcc tgacgtcgca cctgaatcct gcccgagaaa tgcggaatac actcccatgg
    40681 tatcctggat aagtttttcc gattcctgca tgacctgaaa ctgctggctg gcaacctgaa
    40741 aatcctgctc aacccgaaaa acatctgcga cacttttctg attttttcgg accggattca
    40801 gtttaataat gccatccgga cgttcgatct gctccatcag gtcgttgtct gacaactggg
    40861 tggcatcctc gtccataatc acgcgtttgg cctgaagcaa ccaggtcagc ttgatacgac
    40921 gaaaattcac ctcatcctgt gccggaatgg cgcgggaaat tagcccgtat ggctccccgg
    40981 ttttatcctt tcggtatccc cagaaaggaa ccagcggaaa catcccctgc ggagcactac
    41041 aggggcgatc cacaataaag tgcggcccga caaaccaggc ttcacgaata cggcttaccc
    41101 gcccgacttt cacctgaacc cgcccggatg ccacagctac cgcctgcatc agattatttt
    41161 tatcaaaggc caccacccgt ccattactga gttcaatcac cggaagacgc tcgaatgtac
    41221 ggtaataaac cacctgaagc agcacacgac ggcgttcacg ctgaagccat tcgttctgct
    41281 gtcgatccca tgactgatac tcttcccatg cactcatcaa cggactgggc tggccttcag
    41341 taaccgtggt atcgacaaaa ccacgccagt catcaatggc ataatcgata acctgagcca
    41401 ttcccgggaa tgtagctttt gcctcatcgg tatccatcca gcggcgacgc atcagccagc
    41461 ggcagtcact taaatcagcc tcccggctca gccagtccca gaaaacctca ttccggctga
    41521 cagtagacac cttaaattca ggcccgaacg gatcactgtt tcgtctgacc tccacccaac
    41581 tgaggcccgc cttgatttgt tccgcatagg catcggagcg ggccttattc atgttgccaa
    41641 gacggcacgc atcagcaaac tccgcattaa tggcctctgc cagtttctct gtttcatcgt
    41701 tcggatcgtc tgacatcact atcaggtccg ttcttgtttt tgcctccatt cccagtacac
    41761 catctaccgt gggggcaatg aggttatgga tggtcatggg ctgaccgcga tctttcagta
    41821 cctggataac ttccggtgcc agctggtcgc catcataata cgcacaggcc ttgtttgcgg
    41881 catcacgcca gagaggctga ctgtcaatat cagaacagag agacagtaac tgacgctgag
    41941 aaaaacgcgg cgtggatcca tgatcgtttt tcatcgctgt ggtgttaatt tcatttttca
    42001 tcagtgtgcc atccagtgtg tggttctgcg tttatccgtt ttctgtttta ccctcaccgg
    42061 cattctggcg cgcatctcct gggcaatcat gtagctcatg agctgatcat caaagcagcc
    42121 ttcctgtgca ttcatggagc ctttcgcgtc ataaacgtag gtgttcattt ccgataatgt
    42181 gcctgaccag cggatccctg atattccatt attcagaagc gttttcattc cttcggtcag
    42241 aacaggtttg ctctgacggg ttgtcagcca gccaaggcgg ggcgtatcgt cgtcatatgc
    42301 ctggtcaaga tgctgttcgt tgtagatata acgtgtcgga tagagttccc ggagtttcag
    42361 gataactgca tgtccgtgat tattacgctc cggccccaca aacgcgttgt tatacatacg
    42421 acagacctgc gaaatgagat gagcaaaaag ttcagcatcg agatgcccga accagtgagc
    42481 cacctgctcg ccattactgc gtttgacaac atccagcgat gagcggtctc cgtgctccag
    42541 cccttcggca gtatctgccc cacaaacata ctcttcatcc ggatccggca gttcccatac
    42601 cagcagataa ttcatcagcg tccgctgcaa ctcgttttta tttccttcac gcagagactg
    42661 agctttagtc ttcgctcctg taacaggttc aatgtcataa acaatcatcg gtggcgaaca
    42721 gaatgattct gcctgcaacg tactttcggc actgaacaca cgtcgtccgg acgtcagaaa
    42781 cgcctcctgt ggcgttgagg gaaactcctg cttcatttcc tcacgctgtt cagtttcctt
    42841 attgatgtac cactgcttct gttcatcagt aagcgtgatg ttcattgcct tctcaaccgc
    42901 agaaaaatac gtcatttttt cccgtgacag cttcagcccg ctttccggca ctctggcgct
    42961 gtatttagga tcctgccacc aggcgtaaaa atggaattta taatcctgtg ccgtcagcaa
    43021 taagcctgat gcagtgatct cctgtgctcg gttactcatc tcgtaaaaat caccacccac
    43081 gccttcagca gtggactcat caaaaataat gcattcatca gagacggcat taagcgtacc
    43141 ggttcgcagt tctttcgcct tagccggata tttcgcgcaa attttgccgt gctctgagat
    43201 atgcaggcgc tgcaccgtac ctgaacggaa tgaggttgcc acctggatac tcgagccgtg
    43261 accaaacagg atatagccac cgctggcacc gctacgacgt tcaacgatgg tgaatgaggc
    43321 tctcagccag tcagggagat gatcaaacgg tacagcaatt tttgtgcgga aaatttcact
    43381 ggcagcctgt ttatcctgag cgacgatccc gcatttgaga tgcggaatga ataatgcctg
    43441 gtcgagaaga taaatatcaa tggctgtgga aaatcccagc tggcgcgctt tcaggataat
    43501 atttttattg tgcatgctcc ggaacaactg gcgctgcgcc ggtcgcattc tgaaggtgac
    43561 cagttcacct ttttcgttct gtattttgta gagatgattg agccgccacc agggattgct
    43621 cagtttagtc atgatgaaca gacgttgttc agcctcggtc atttctgacg gctcatcaca
    43681 tcgcggttca ttcttccgga atgtcatcca gtctccccga attactcatt tcatgcagcg
    43741 atgacacgat gtcactgaca ggcgtaacaa cgccccgacg ctggctggtc agaatatcgg
    43801 tttccgctct gagtttatct ctggcggcgt tgattctttc ccggtcagca cgaagttttg
    43861 gtgctgtctc agccaggacg tccagcgtca gcaatgagcg ttcaattgac tcgatacggg
    43921 caatattccg gtcaagggcc tgttcagctt tgagtatttt gtcgtaaaga gcaacgcggg
    43981 tttccacgtc agttgcctct tccaggtcgg cgaacatccc tttaagtgcc ttagttactg
    44041 aaagtgcgcg ggcccgggtg aacaccagtt catcgaacag caccatgtcg gacgcatcat
    44101 ccatgaggtt atctgcctca agatacttcg catatccacg gtgtcttacg gcgtgggtgt
    44161 ttcgctgaga aaaagcgttt gaaggtggta aaagtcggga accacgaatc cgtttcgttt
    44221 ctgccgaatt tgcgcagttt ttttcagagt tttttgcgca tttttcatcg ccggaacccg
    44281 cgtcattgca gggttcttca tctgagatgt catgatcgat ttcatgatcg gttttatgat
    44341 caatttcatg atcgattttg cccattttta tacgggttct ggcggtgttg taattaatct
    44401 ttttcttccg gcaccagtcc agtaatgtta ttcccgtttc ggcatgttcg cgtcggaatg
    44461 cctgctccag ctttttccag tccagctttg ccatgtcact ttctgacgtc ctgtgttaaa
    44521 aactgatgca taatgaccgc tgtgattttt cagatttcac acagcagcac catatttgat
    44581 cgatatttgc acaatgcggt tgttttatcc ggtttcttcc accaccgcac cggacaggcg
    44641 gcttcgcggg aaatcgctcc catctcgtga aaaatgagaa aacccggtgt gcatcgtttt
    44701 tgattatccc cgcacactca cgcagataag gtggctgcag tctctgttaa tgcgggaata
    44761 cggcgacgat acggcgcatg gctatgtcag gctgaaatgc ctttatcaaa tccgggtaac
    44821 gcaatctgcc cctgctgctc cagcctgtcc agccttgcca gcaactgagg cttcttcacc
    44881 ctgccccagc gattgagcaa acgacctgac atactggcga catctttctc tttcatgtac
    44941 tccagcatta cagcgttgcg ctccgcctcc agattcgcca gaccttgctg aataagctca
    45001 gccatccagt tgaaagcctg aatgtacgct tccttaaagg tcatagcagc cttccccgta
    45061 aaaccaaata ccaacatcgt ccaaccgtct tttgtcattc gatatatggg ctggggttta
    45121 ccgttctgta actcattgtt ttcatagcaa agcgcaaaat tgcgctctgc aaattctttt
    45181 gagcattgtt gaacaacggc tcttgttttc cgcaatacat ctttgtgttc tttgccaaat
    45241 gctacggcaa cctttcgggt atccgtcacc ggctcgttgc ctgtcacaaa aacaagatct
    45301 cgaaaatcga tgccgttaac gatagttgga taattcatca gtgcacacct tttagtgatg
    45361 aaccttgtca cacaggattc cggcccacag aaaggcaccg atcaccaaac cggcatcctc
    45421 aagggtcatc ctgaaaggtt ctgtgttcat aagtcgcgcg tgtgaagcgc gtttactgcg
    45481 gacataaaaa agccccgtat cgcgaggctc attaaattga ctttgtgatt tgcaaaaaaa
    45541 ttatttcagg cattgcgtcc tgatgtattc ctgcaggtag ttaacctgcg cagttatctt
    45601 gtcgattcca cttctgagac ggtaataatt gagttcagca tctgctgtaa gtcctgggct
    45661 ttctccatcg cccatgctgc tggctccggt cgttgacttt gcacaggtgg cggcgacttg
    45721 caggcgctta cgcccagcag aaacatcagc acggagactt tcgatagtcg cattagcatc
    45781 agcaagctcc tttgtgtatc tggcgtcaag ttctgctaca tcacgttgcc gcttctgcat
    45841 atctgcgatg atggatgtgg ctttatcgcg ctgctctttg taagcgatgg cgttatcacg
    45901 gtaatgattc agccccagac taagcgcacc acagaccacc agcagaataa cggtaaacgc
    45961 ggaaagcatt cggtttatgc tcaccccacc agccctgccg aagtcaacgc catccagttt
    46021 atggaaagaa aaagaacaac cagcattagt gaaaatgaaa taccgacgat tacacaaagg
    46081 cccttcgcca gcgttatgag tttatccgat atcattagcc accactccat caatccgcct
    46141 ttgttatttt ccctttgcct gtatcagcca ggacaaaatc aatcagcaga ttcgcttcgt
    46201 ttatcaatgt gcggattttt gatacatgcg cggctttaac ctgtttccac tcattcagcc
    46261 cggtagcaaa cacactggca atgtttttat cccgtttcat gtcagcgcag gcctgattga
    46321 gttcttccat cacactcatt cgacggggat taacgacacc ccccttcgtc cagtattcat
    46381 agagaacatc gtcgcactct tcctgatact ggattacctt gtcgcggatt tcgggtttta
    46441 ctttgttggg attgatggtt tgtaaccagc ctgcaagttt tcgaagtggc agggacacca
    46501 tattgcgtcg tttcccatcc tcagcaacca taacgatttc cgttatagtt gacgcaaaac
    46561 gctgtcttaa cttagccaac tgtgattgcc aggccagccc catccccgca acgacaggtt
    46621 tcatgggaac gtatggctca ccgttatggt taaccacata aagagaatcg ccgtgaaacg
    46681 gcacggtcat catattcatc ggttatttcc ttttagtgat gaaccctgcg cacaggaata
    46741 accagcccaa agagggttaa ccagaccact gccggttatc caccagggct catcctgaaa
    46801 ggttctttgg tttatttacg cttgtgcgaa gcgcagaaat gacaaagaca ccattacggt
    46861 gcctcagcgt gaaacaatct tcctgacttt attcacttgc attttgccag ttcgcaggat
    46921 ttcgtgttat ccgtccgcgt gagcaaacgt catttttcag caaaatattc tgctacctgt
    46981 cgatacccca gcatgccagc gcactctcct ggtcgcgacg ggatacctga ccgtagcaat
    47041 tatttgagcg gatacggcag tctctgccac cgtccttaat ccaccagcga atcgcttcac
    47101 acgctcccct gcgatcgcct gcattaattc gtttataaaa cgtcgacggg aaacacttac
    47161 cggggccaat gttgtaagga cagaatgacg cgatccccgc tttctgaggt tcggtcagtg
    47221 gcactctgat gtttttcgcc acccatgcca gcgccttatc acgttcaatg gcgttaacct
    47281 ggtcgcattt ttccttcgac aacttcatgc cggggacgac aagcttacca tccacctggg
    47341 tggcaccacg gcagatggtc cagataccca caccatcacg gtatgccgtg gtgtggttgc
    47401 cttccttttc atccagaaac tggtcgagga tttcaggcgc agaagcacct gcggcaatca
    47461 gcgccagaac ggcagccgat aaaccatagc ggagtttcct gctcatcagc ttactctccc
    47521 cgtgccgcct tacgcctgtc ctctctgatt ttgaaataca ggttcgtcag gtacgtcagc
    47581 ag
//
LOCUS       DAZFTN010000018        46307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFTN010000018 DAZFTN010000000
VERSION     DAZFTN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..1682
                     /gene="yegH"
                     /locus_tag="ACHKV2_001262"
     CDS             99..1682
                     /gene="yegH"
                     /locus_tag="ACHKV2_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6939184.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1757..2095
                     /locus_tag="ACHKV2_001263"
     CDS             1757..2095
                     /locus_tag="ACHKV2_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6939185.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2085..2375
                     /locus_tag="ACHKV2_001264"
     CDS             2085..2375
                     /locus_tag="ACHKV2_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6939186.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2428..4281)
                     /gene="asmA"
                     /locus_tag="ACHKV2_001265"
     CDS             complement(2428..4281)
                     /gene="asmA"
                     /locus_tag="ACHKV2_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO6939187.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4303..4884)
                     /gene="dcd"
                     /locus_tag="ACHKV2_001266"
     CDS             complement(4303..4884)
                     /gene="dcd"
                     /locus_tag="ACHKV2_001266"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO6939188.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4976..5617)
                     /gene="udk"
                     /locus_tag="ACHKV2_001267"
     CDS             complement(4976..5617)
                     /gene="udk"
                     /locus_tag="ACHKV2_001267"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO6939189.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5935..9253
                     /gene="dgcE"
                     /locus_tag="ACHKV2_001268"
                     /pseudo
     CDS             5935..9253
                     /gene="dgcE"
                     /locus_tag="ACHKV2_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9354..10202)
                     /gene="alkA"
                     /locus_tag="ACHKV2_001269"
     CDS             complement(9354..10202)
                     /gene="alkA"
                     /locus_tag="ACHKV2_001269"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO6939190.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10336..11688
                     /gene="yegD"
                     /locus_tag="ACHKV2_001270"
     CDS             10336..11688
                     /gene="yegD"
                     /locus_tag="ACHKV2_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6939191.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11701..13641)
                     /gene="yegI"
                     /locus_tag="ACHKV2_001271"
     CDS             complement(11701..13641)
                     /gene="yegI"
                     /locus_tag="ACHKV2_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO6939192.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13638..14399)
                     /gene="pphC"
                     /locus_tag="ACHKV2_001272"
     CDS             complement(13638..14399)
                     /gene="pphC"
                     /locus_tag="ACHKV2_001272"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO6939193.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14396..15055)
                     /gene="yegL"
                     /locus_tag="ACHKV2_001273"
     CDS             complement(14396..15055)
                     /gene="yegL"
                     /locus_tag="ACHKV2_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6939194.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15276..15335)
                     /gene="ibsA"
                     /locus_tag="ACHKV2_001274"
     CDS             complement(15276..15335)
                     /gene="ibsA"
                     /locus_tag="ACHKV2_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO6939195.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15607..15663)
                     /locus_tag="ACHKV2_001275"
     CDS             complement(15607..15663)
                     /locus_tag="ACHKV2_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6939196.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15935..15991)
                     /locus_tag="ACHKV2_001276"
     CDS             complement(15935..15991)
                     /locus_tag="ACHKV2_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6939197.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16271..17518
                     /gene="mdtA"
                     /locus_tag="ACHKV2_001277"
     CDS             16271..17518
                     /gene="mdtA"
                     /locus_tag="ACHKV2_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO6939198.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17518..20640
                     /gene="mdtB"
                     /locus_tag="ACHKV2_001278"
     CDS             17518..20640
                     /gene="mdtB"
                     /locus_tag="ACHKV2_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO6939199.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20641..23718
                     /gene="mdtC"
                     /locus_tag="ACHKV2_001279"
     CDS             20641..23718
                     /gene="mdtC"
                     /locus_tag="ACHKV2_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO6939200.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSIAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23719..25134
                     /gene="mdtD"
                     /locus_tag="ACHKV2_001280"
     CDS             23719..25134
                     /gene="mdtD"
                     /locus_tag="ACHKV2_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939201.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25131..26534
                     /gene="baeS"
                     /locus_tag="ACHKV2_001281"
     CDS             25131..26534
                     /gene="baeS"
                     /locus_tag="ACHKV2_001281"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO6939202.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIAPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26531..27253
                     /gene="baeR"
                     /locus_tag="ACHKV2_001282"
     CDS             26531..27253
                     /gene="baeR"
                     /locus_tag="ACHKV2_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO6939203.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27444..27776
                     /gene="yegP"
                     /locus_tag="ACHKV2_001283"
     CDS             27444..27776
                     /gene="yegP"
                     /locus_tag="ACHKV2_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO6939204.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27924..29285
                     /gene="yegQ"
                     /locus_tag="ACHKV2_001284"
     CDS             27924..29285
                     /gene="yegQ"
                     /locus_tag="ACHKV2_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO6939205.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29615..29932)
                     /gene="yegR"
                     /locus_tag="ACHKV2_001285"
     CDS             complement(29615..29932)
                     /gene="yegR"
                     /locus_tag="ACHKV2_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO6939206.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            30338..31237
                     /gene="yegS"
                     /locus_tag="ACHKV2_001286"
     CDS             30338..31237
                     /gene="yegS"
                     /locus_tag="ACHKV2_001286"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO6939207.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(31319..32092)
                     /locus_tag="ACHKV2_001287"
     CDS             complement(31319..32092)
                     /locus_tag="ACHKV2_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6939208.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(32198..33238)
                     /gene="gatD"
                     /locus_tag="ACHKV2_001288"
     CDS             complement(32198..33238)
                     /gene="gatD"
                     /locus_tag="ACHKV2_001288"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6939209.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(33286..34641)
                     /locus_tag="ACHKV2_001289"
     CDS             complement(33286..34641)
                     /locus_tag="ACHKV2_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6939210.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(34645..34929)
                     /gene="gatB"
                     /locus_tag="ACHKV2_001290"
     CDS             complement(34645..34929)
                     /gene="gatB"
                     /locus_tag="ACHKV2_001290"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO6939211.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(34960..35412)
                     /gene="gatA"
                     /locus_tag="ACHKV2_001291"
     CDS             complement(34960..35412)
                     /gene="gatA"
                     /locus_tag="ACHKV2_001291"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO6939212.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(35422..36684)
                     /gene="gatZ"
                     /locus_tag="ACHKV2_001292"
     CDS             complement(35422..36684)
                     /gene="gatZ"
                     /locus_tag="ACHKV2_001292"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO6939213.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(36713..37567)
                     /gene="gatY"
                     /locus_tag="ACHKV2_001293"
     CDS             complement(36713..37567)
                     /gene="gatY"
                     /locus_tag="ACHKV2_001293"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO6939214.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(37866..38918)
                     /gene="fbaB"
                     /locus_tag="ACHKV2_001294"
     CDS             complement(37866..38918)
                     /gene="fbaB"
                     /locus_tag="ACHKV2_001294"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO6939215.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            39175..40452
                     /gene="yegT"
                     /locus_tag="ACHKV2_001295"
     CDS             39175..40452
                     /gene="yegT"
                     /locus_tag="ACHKV2_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO6939216.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            40449..41453
                     /gene="yegU"
                     /locus_tag="ACHKV2_001296"
     CDS             40449..41453
                     /gene="yegU"
                     /locus_tag="ACHKV2_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6939217.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            41450..42415
                     /gene="yegV"
                     /locus_tag="ACHKV2_001297"
     CDS             41450..42415
                     /gene="yegV"
                     /locus_tag="ACHKV2_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6939218.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(42389..43135)
                     /gene="yegW"
                     /locus_tag="ACHKV2_001298"
     CDS             complement(42389..43135)
                     /gene="yegW"
                     /locus_tag="ACHKV2_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6939219.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(43187..44005)
                     /gene="yegX"
                     /locus_tag="ACHKV2_001299"
     CDS             complement(43187..44005)
                     /gene="yegX"
                     /locus_tag="ACHKV2_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO6939220.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(44070..44870)
                     /gene="thiD"
                     /locus_tag="ACHKV2_001300"
     CDS             complement(44070..44870)
                     /gene="thiD"
                     /locus_tag="ACHKV2_001300"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO6939221.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(44867..45655)
                     /gene="thiM"
                     /locus_tag="ACHKV2_001301"
     CDS             complement(44867..45655)
                     /gene="thiM"
                     /locus_tag="ACHKV2_001301"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO6939222.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(45690..45786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45989..46228
                     /locus_tag="ACHKV2_001302"
     CDS             45989..46228
                     /locus_tag="ACHKV2_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFO6939223.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT        11006 a        12234 c        11834 g        11233 t
ORIGIN      
        1 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
       61 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
      121 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
      181 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
      241 ttaccgggct gttgctggcg atgttaatgc gcctgttact gctggcgtca atctcctggc
      301 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
      361 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
      421 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctgggggg
      481 tggtgacaca aattgtggta ctggacgcca tcttctcact cgactcagtg attaccgccg
      541 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
      601 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
      661 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
      721 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcgctca
      781 atcagttggc tatctttaac cggcgacgtt ttctttccgc taaccagaca ctgcgccagc
      841 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaggatgcg gaactggacg
      901 ccgaaaccgc gtccatgctg gtggatcatg gtaaccagca gatctttaat ccgcaggaac
      961 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
     1021 cgcgccacga tattgagcat atcgatctca acgcgccgga agacgagatc cgccagttgc
     1081 tggagcgaaa tcagcatacg cggctggtgg ttactgatgg cgatgacgcg gaagatttgc
     1141 tcggtgttgt tcacgttatc gacctgttac aacaatcact gcgcggcgaa ccgctcaacc
     1201 tgcgggtgtt aattcgccag ccgctggtgt tcccggaaac cttgccgttg ttacctgccc
     1261 tggagcagtt ccgtaatgcc cgcacacact ttgcttttgt ggtggatgag tttggctcgg
     1321 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     1381 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     1441 cgaatggtca tatgccgctg gaagatctgg tgcaatatgt gccgctgccg ctggatgaaa
     1501 aacgtgaata tcacaccatt gccgggctat tgatggaata tttgcagcgt attccaaaac
     1561 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     1621 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     1681 gataaaacat caaatgcgca ggttcaaact gcgcatttga tttatacaaa atttagtata
     1741 atctaaggat ggagtaatga gaaaaaagct ggcttttctt gataccagtc ttgacgattt
     1801 acgcgcattt ccagaatcgt ctcgacagga aattggctat caactcgaca ggatccaaca
     1861 agggttaaac ccatacgact ggaaaccttt ttcaactatc ggccctggtg tgcgagagat
     1921 tcgtactcgc gatgctgatg ggatctatcg cgtcatgtat gtcgcaaaat ttgaagaagc
     1981 ggtttacgta ttgcattgtt ttcaaaagaa aacacagact accagtcaat cggatattga
     2041 tttagccaaa cgacgctata aagaacttgt tcaggagcgg aaaaatgaaa attgaaacct
     2101 ttgatagtgt gtgggatgcg gtgagtgaca cccctgaaca agcggaaaat atgcggatcc
     2161 gtgccgagct tgtaacgatc attaataact ggattgaaca acaagggttt agccaggctc
     2221 aggcagcttc tgctttaggg gtcactcaac ctcgcatctc tgaacttgcc agaggtaaaa
     2281 tccagatctt tagtatcgat aaattgatca ccatgatggc tcatgcagga ctacatattc
     2341 aacgtattga agttcaatat ccacatgctg cttagtgcta taaatacaga tggcgacaaa
     2401 ttgatgccgc catctatata acttgtctta catcttctcc agcaacttct tcacatcttt
     2461 gccattgcgg gaatctttat tccgctcggc ccagtcattc aggcgacgtt tcgcttcgtc
     2521 ctgtagatgt ttgcgcagta actgatccac ttgcagactg taattgagtt gctgccagtt
     2581 gccataaacc cgcagcggta ctggcgtttc tttgaggaaa tcaatcagtt tgctttcccc
     2641 gttccagcca cccacgaccc gaatatcaaa ctgggtgtcg caggtttgat ctgccagatt
     2701 caacatgcct tcccctgtaa gcgccagcac aggcgattga ccttgcatgt cgtttaacgt
     2761 cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc
     2821 gaagttttca gcggccttca catcaccacc attacgttct accgcttgct gaatcatctg
     2881 ctggaagttc atcccttcca tgcgcgtatc ggccatttcg acatgtgcct gtccttgcca
     2941 gttgtggcgg aatgcgtcgg catctatgtc agcaccggag aaatcaccag ccaatgacat
     3001 ttttccggtc aacgaaatcg gatagttaaa cgccttcaga atggtgccaa tctcaacgtt
     3061 ttccagccgt ggctggaagt ttatccgcgg atttattgat gtcgcgtcca gcgtgcccgg
     3121 cagtgaaacc tgtccaccgt taagcttgcc ctgtagttga gtaatttcca gcaaacccga
     3181 cttgttggtc atttgcgtgg caacatctgt aaaattcatt ccgcgccagc gcacgttact
     3241 ggcctgcaac aaaatatcag ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat
     3301 acgcgaagaa ataaccgggc gcggcaacgt tgattggctc tgcccctgct gcgcggcacc
     3361 gttttcacca ttcgcggttt cattaagcgg gatgaggttg tcaagattca gttgcgggaa
     3421 ttgcagcctc agctgccatt ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag
     3481 cgtactatca ttggcggtca aactaatttg gttaaatgaa aggcgtttac gtgactcctg
     3541 ccactgggcc tggaaactcc cctgcccctg aataccttgt tttggtaaat cggcaccctg
     3601 caactgccag ttaatttgtt caatagccgc cgttaaatca tgcggataat cagaagcatc
     3661 taccgtaccg ttaagggata tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa
     3721 ctcaaatgag ccacgatgtt ggggatcttg ttccatttgc aggcggatat tgcggattgt
     3781 cacctgctcg tcatcttcat gctggaacac cagcacgctg tccgccacct taagactgga
     3841 tatatcaaac gaccatccgc gatcgtctga cagatccggc aaggtattgt cgcgcggtgc
     3901 aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc gtcagttgga tcactgcccc
     3961 ttttagcatc acctgcttaa cgctcagttg atgactcagt agtggtaaaa gcgccacgtc
     4021 caggcgcatg ttgtcggcgc gaaccagtgg ctggctcgcg ccctgggcgg tgagagacat
     4081 tcgcccggag aggatactaa gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg
     4141 caattgataa ccgctacgtg cagcaacttg cttgaccata tagtcgcgga aatcattcgg
     4201 attcaccagc aacactaacg cagataaccc ggccaccagc acgaccagga gtatcatcag
     4261 cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg aattagtctt tatcgattcg
     4321 gctggctacc gcgccctgct ggttgcgata cttcgcatct tcacggcggt tgtaaggtcg
     4381 cgccgccggg ccggaaagcg gctcaaagct cagcgcacca attaacatgc ccggacgcag
     4441 cgccagcggc agcttaccgg agttgtagaa ctccagcaca atgcaaccag accagcccgg
     4501 atcgatacgg tgcgcggtga cgtgcaccat cagccccaga cgcgccagtg aggaacgccc
     4561 gtccagccag cccaccagat cggctggcag cgtcaccgac tccagcgtca ccgccagcgc
     4621 cagctctcct gggtgaagat agaacgcctc gctttcatcg agaacgatct catcgctcat
     4681 cacgcggtca agcgcggcgc tcacttcatc tttgggaccg ctcagatcga taaacgctgc
     4741 cgtgtgacca cggaaggtac gaaatttatt gcccaggcgt acatccaccg tcgcgccgtt
     4801 aatacgctcc actggcggac gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc
     4861 aatatctcgg tcacacagac gcatggcatt ttctcctttc gcgcatcact cccttaacgc
     4921 caattacgtc aagggcatac caggttatca ctgaacggta cacaatttat caagcttatt
     4981 caaagaactg acttattttc gctttcaata tatcgatcgc gatgcggttt ttcccgccgc
     5041 gcggcacgat aatgtccgca tattgtttag aaggctcaat gaattgcagg aacatcgggc
     5101 gcacggtttt ttgatattgc gccatcacgg aatccattga acgcccacgc tcgttaacgt
     5161 cacgcttgat gcggcgcatc aggcagatat ccagcggggt atcaacgaaa atggagaagt
     5221 taagttcgtc acgcaaacgt gcatccgtca gcaacaaaat gccttcgaga atgatgacct
     5281 tcttcggctc aaccgtcacc gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg
     5341 gcaggtcaat tgccgagccg cgtttcaacg cttgtaaatg ctcaagcagc aggctgtgat
     5401 ccatcgcgct gggatggtcg tagttggtct taacgcgttc ttccatcgac agatggcttt
     5461 gatctttgta atagcagtct tcgggaatta cgccgatgtg ttcatcaccg acttgctcac
     5521 gtaattcacg ataaagggta ctggcaataa gactcttgcc ggaagccgat gcgccagcga
     5581 taccgataat gacgcactga tgagactgat cagtcatata tttagcgacc tgattaacct
     5641 ggatgttagg aagggggcga cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc
     5701 tgataccagt cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacga agaattcccg
     5761 gtggcaaatt acgttgatca gttttataca aggtaaaaat tgttatacgc agttgcgcaa
     5821 attatccgcc tttacgtcac tttatgagca attcgcatat aaaatgtaaa acttttgtac
     5881 tagcataaac acagaaacga atactggcga cctggccttg cggataaagc ggtaatgagc
     5941 aaacaatcac agcatgtatt aattgccctg ccccacccgc tgcttcacct ggtcagttta
     6001 ggtttagtct cgtttatctt cacccttttc tcgcttgagc tttcgcagtt tggcacccaa
     6061 ctcgccccac tgtggttccc gacgtccatc atgatggtgg cgttttatcg ccatgccggg
     6121 cgcatgtggc cagggattgc gctgagctgc tcgctgggaa atatcgccgc atccatcctg
     6181 cttttttcca ccagctcgct gaacatgacc tgcacgacca tcaatattgt tgaagccgtg
     6241 gtcggggcag tgctgctacg taaattgctg ccgtggtata accccttgca aaatctggct
     6301 gactggctgc gtctggcact cggcagcgcc attgttccac ctctgttggg gggtgttctg
     6361 gttgtcctgc tgacgcccgg agacgatcct catcagggca tttttgatat gggtactgtc
     6421 agaatccatc ggcgctctgg cactggtgcc gctgggattg ttatttaaac cacactatct
     6481 gctgcgccat cgcaacccac ggttgctttt tgagtcgctg ctcacgttag ccatcacact
     6541 gacgttaagc tggctttcga tgctgtatct gccgtggcct tttactttca ttattgtgct
     6601 gttgatgtgg agcgccgtgc gcctgccacg aatggaagcc tttttgatct tccttaccac
     6661 ggtgatgatg gtgtcgctga tgatggccgc ggatccctcc ctgcttgcta cgccgcgtac
     6721 gtacctgatg agccatatgc cgtggctacc gtttttgctg atcctgctgc ccgccaacat
     6781 catgacgatg gtgatgtatg cctttcgtgc ggaacgcaaa cacatttccg aaagcgaaac
     6841 ccgttttcgg aacgccatgg aatattccgc catcggcatg gcattagtgg gcaccgaggg
     6901 acaatggctg caatccaaca aagcactctg ccagtttctc ggttacagtc aggaagagct
     6961 gcgcggactc acctttcagc aactgacctg gccggaggat ctcaataaag atctccaaca
     7021 ggttgaaaag ctgataagcg gtgaaataaa cacctattca atggaaaaac gttactacaa
     7081 ccgcaatggc gatgttgtct gggcgttgct tgccgtctct ctggtgcgcc acacggatgg
     7141 cacgccgctc tattttatcg ctcagattga agacattaac gagctaaaac gcaccgaaca
     7201 ggtgaatcag caactgatgg aacgcatcac gctggctaac gaagcgggcg ggattggcat
     7261 ctgggagtgg gaactgaagc cgaatatttt tagctgggat aagcggatgt tcgagctgta
     7321 tgaaattcct ccgcatatca aaccgaactg gcaggtgtgg tacgagtgcg tgctgccgga
     7381 agatcgccaa cacgccgaaa aagtgattcg tgattcgttg caatcacgct cgccctttaa
     7441 actggaattt cgcattgccg tgaaagatgg catccgccat atccgcgccc tcgccaaccg
     7501 ggtactgaat aaagaaggcg aagtcgaacg cctgctcggc attaatatgg atatgactga
     7561 ggttaaacag cttaacgagg cattgtttca ggaaaaagag cgcctgcaca ttacgctgga
     7621 ctccatcggc gaagccgtgg tctgtattga tatggcgatg aaaattacct ttatgaatcc
     7681 agtggcggag aagatgagcg gctggacgca ggaagaagcg ttaggtgttc cgctcctgac
     7741 ggtgttgcat attacttttg gcgacaacgg accattaatg gagaacattt acagtgccga
     7801 cacctcacgt tccgcgatcg aacaagatgt ggtgttgcac tgtcggagcg gcggcagcta
     7861 cgacgtgcat tacagtatta cgccgttaag tactctggac ggcagcaata taggctcggt
     7921 actggtgatt caggacgtta ccgaatcacg caaaatgctg cgccagctga gctacagcgc
     7981 ctcccatgat gcactgacgc atctcgccaa ccgcgccagt tttgagaaac aactgcgtat
     8041 cctgctgcaa acggtaaaca gtacgcatca gcgacatgca ctggtgttta tcgatcttga
     8101 tcgctttaaa gcggtgaatg acagcgccgg gcatgcggcg ggcgacgctt tgctgcgcga
     8161 actggcgtcg ttgatgctga gtatgctgcg ctccagtgac gtgctggcgc ggctcggcgg
     8221 tgatgaattt ggtctgctgc tgccagactg caatgttgaa agcgctcgtt ttatcgctac
     8281 acgtattatc agtgctgtga atgactatca ctttatatgg gaaggccggg tacatcgggt
     8341 aggtgccagc gccgggatta ccttgatcga tgacaacaat catcaggcgg cagaagtgat
     8401 gtcgcaggct gatatcgcct gttatgcctc caaaaatggt ggccggggcc gggtgacggt
     8461 ttacgaaccg cagcaagctg ccgcacatag cgaacgggcg gcgatgtcgc ttgatgaaca
     8521 gtggcggatg attaaagaga atcagttgat gatgctcgcc cacggtgtcg cttcgccacg
     8581 gatcccggaa gcgcgtaatt tgtggctgat ttcacttaag ctctggagtt gcgaaggcga
     8641 gattattgat gaacaaacat ttcgtcgtag cttcagcgat ccggcgctta gccatgctct
     8701 tgaccgccgg gtattccacg aatttctcca gcaggccgca aaagcggttg ccagtaaagg
     8761 cataagcatc gccctccccc tttccgttgc cggtttgagt agcgccacgc tggtgaatga
     8821 tctgcttgag cagctggaaa atagccctct accaccacgg ttattacatc tgattattcc
     8881 ggcagaagcg attttcgatc acgcagaaag cgtgcaaaaa ctgcggctgg ggggatgccg
     8941 gatcgtattc agtcaggtgg gccgcgatct gcaaatcttc aactcgctga aagcgaatat
     9001 ggcagattac ctgctacttg atggtgagtt atgcgccaac gtgcagggta atttgatgga
     9061 tgagatgctg attacgatta ttcaggggca cgctcagcga ctcgggatga aaaccatcgc
     9121 cgggccagtc gttttaccct tagtgatgga tacgctttct ggcatcggcg tcgatctgat
     9181 ttatggtgag gtgattgccg atgcccaacc gctggatttg ctgatgaata gtagttattt
     9241 cgcgattaac tgagggagcg tttgcccatt gcctgatgcg acgctaacgc gtcttatcag
     9301 gcctacaaat cgctcattcc gcaaggcgct cgcaccgcat ccggcgacca acgtcatgct
     9361 tcgtctggtt gccagccttc cgtataccag atatgcaaca gcgcataaga acgccagggc
     9421 ttccagcgct cggcataacg gcggatttgc gccggtgtca ttcccggaaa tcgctgttta
     9481 atcagataat catccggcag gaaaacatct ttcgcctgcc agccacgcaa agcaaaataa
     9541 ttcgccgtcc agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc ctgctccaca
     9601 tcgcccggta ttgtcattgg taaggtgccc tccagcgccg catttgccag atgaatcagc
     9661 gcctctgccc gtttcaacgg catacctaac gcttttaatg cctgcgggtc tgctgctgcc
     9721 agccgctgag gcgtggggaa gcagatatac tccggaaaat catccagccg ttcgccataa
     9781 agctgtacca ctttggcagt caattttgcc gccatcgcca cgctcaccag ttggcctaaa
     9841 atcgcccgca cgccttgctc aaaagcatca acactgccgg gtaaacgcaa tccgggccgc
     9901 gccgcgccta acttgcccaa cgcaccgtta acaatctgcg ggttacattg cagatcaagc
     9961 aggcggctca ttttcgccag acactcagcg gcaacgggtt ctaaatctgc acttaaattt
    10021 atgtgcagag tatggcgggc tatatccgga atagcagtca ccacgccgcg atattcgccc
    10081 accgccagac tacgggcata ataatcgtca gcgaccgttt ccacaccact cactgcacgg
    10141 gcggcgagaa atcccaacat ccacgaccag tcatacggcg gctgccagtt cagggtatac
    10201 atcgcatctc cttattcatc cgctttcagc ataaacgtta ttcagacgct gcgctttgct
    10261 ttcatattcc ggttgtcgcg acggcaacat ttcgctaaag tcacgcccct tcttcaccgg
    10321 catggggatt atttcgtgtt tattggtttt gattacggta cagcaaactg ttcagtagcg
    10381 gtcatgcgtg acggtaaacc gcacttgcta aaaatggaaa acgacagcac gctgctgcct
    10441 tcaatgcttt gcgcgccaac gcgtgaagcg gtaagcgaat ggctgtaccg ccatcatgat
    10501 gttccggcag acgacgatga aacgcaggca ctgttgcgtc gggcgattcg ttataatcgc
    10561 gaagaagata tcgatgttac ggcgaaaagc gtgcagttcg gtctttcctc actggcacag
    10621 tacattgatg atccagaaga agtgtggttt gtgaaatcac caaaatcgtt cctcggtgcc
    10681 agcggcttaa aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc aatgatgttg
    10741 cacattcgcc agcaggcgca agcgcagctg ccagaagcga ttactcaggc ggtgattggt
    10801 cgcccgatca acttccaggg gctgggcggc gatgaagcaa acgcccaggc gcaagggatt
    10861 ctggaacgcg cggcgaagcg tgccggattc agggacgtgg tattccagta cgagccggta
    10921 gcggctgggc tggattacga agccacctta caggaagaaa aacgggtact ggtggtggat
    10981 atcggcggtg gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt
    11041 ctcgatcgtg aagccagcct gctgggtcac agtggttgcc gtattggcgg taacgatctg
    11101 gatatcgcac tggcgtttaa aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa
    11161 aaaggcatcg ccctgccgat tctgccgtgg tggaatgcgg ttgccatcaa cgacgtacct
    11221 gcgcagagtg atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat
    11281 gcccgcgaac cggaaaaagt ggccctgcta cagaaagtct ggcgtcagcg tttaagctat
    11341 cgcctggtgc gtagcgcaga agagtgcaaa attgcgcttt caagcgtagc ggaaacccgc
    11401 gcctcactgc cgtttatcag cgatgaactg gctacgctga ttagccagca agggctggaa
    11461 agcgccctca gccagccact ggcgcggatt caggaacagg tgcaactggc gctggataac
    11521 gcccaggaaa aaccggacgt tatctacctg accggcggaa gcgcccgttc tccgctgatt
    11581 aaaaaagcgc tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc
    11641 tccgtcaccg ccgggctggc acgctgggcg gaagtggtgt ttcgttaatt gaaaaatggc
    11701 tcagcagcgg cttaaatctg cctgcgcctg agccagtttt tctgccgcct gacgtaacgg
    11761 ttccatcaac ggcatggtcc gtgctggagc atgaaggcgg aatcgctgca actctgtcgc
    11821 gccgacagtc aacgccgatt ccagccgatg tcgtttggcg gccattttcg ccattaccgc
    11881 ttgtctttct gcaggcgtta ccgcttcgtt cggcctgaac acaaaacggc gttcacagct
    11941 cgctttccag tcgataaccg cctgggtcag atgatcacca aaccctttaa cttgcttaac
    12001 cccacgacgg gtaacatccg cggctgtttc aataccaaaa gaccgtaacg ccgctttacg
    12061 cgcagggcca acgccgggaa tagaggcagc atcaataaaa aatccctcca gaaacttctg
    12121 cttctgccgt tcccttgcag tgtcatgaag tgctgccaga gcgcgtttct cctcttccgg
    12181 taacccgaga atttcgtcct tcattttttc gagcatcgcc cgtttggcga taaaaccttc
    12241 cagcccgccc aactgttgga tctggctgac taaatggtca tagtccattt tcgcgcgatt
    12301 aaatgcctca cggcgttgct ggatttctgc tttataaact ttgcttgtca gactgccaat
    12361 aatccagata gccgccagca caggaaccaa aataatataa cgcggttctg cctgaaggcc
    12421 gcaaagcaac agcgataacg ctgacagtgc gatctcaatc agaatgatgt attcgcgccg
    12481 taacaggcct aaaggaagcg gcctgccagt cggttggaaa tgatcgggta atggcaattg
    12541 caatgctggc ggtgctactg acgccatcac catcgcccag actttcgcca gcacaaaatc
    12601 accactggtg gtaatgacct cttcgccgag atcaataaaa tagataacgc cttgattatc
    12661 cagcgcacac cacgggcagt cagtcaaatg accggggtaa acatgcattg ccgaaacggt
    12721 acatttcttt aactgttggc gtagtaaatc cagtgccgct acccacgctt ttgccgtcgg
    12781 acgcgcggtt gccacgccac tttccgtgaa tgcctgctga aacatggctt caacatcgcc
    12841 cggtaacatc gacagcggaa tcgatcgtgg cggcggtttt aaaccacgtc gctggttgtc
    12901 tgacgcgtag gcataacgga aatgggcaat atccgtctcc agcgcattac ccgcatcaga
    12961 gataagcggc acaccagaat aaggatgccg cccaccaaac aagacgtgaa aaatcagcaa
    13021 cgcaaggcca aaattatcgt gattcgcggt acgttcaaaa ccgacaaatg atggcaaggt
    13081 ttgtagctct ggcggcgtaa aatgcgacac gccgacttcg cataaatgca gtgtgccatt
    13141 ggcgttaatc tgaaaggagt cactatcgat caacaccact ttgctgtcgc gacctaccat
    13201 aaagctgttc tggtttacgt cccccacgac gtgcccgtgc tcgtgaaccg tagcaaaaga
    13261 tgaagcaata ttgcgcgcaa catagagtag aaaatcccac gcacaatgag gataactctg
    13321 gcgacgatgt gccgggctat agatcatatg aatcggttct ttaccagaaa cttttggcat
    13381 cataaaaccg ataacttttc cgcctcgtcc accgtgaagc gttgcctgcg gccaggcgac
    13441 ataattcaac aactgcgcgt cagctgtcgc agccataaag gcaagtttgt cctgttttaa
    13501 ggcgggtggc ggcgtgtgat aaatcttggc gacgctatcg acaaactcat tgatatcata
    13561 aaccgcgcct tcgccacctt tgcccagttc acggcctggc gtaacgcact caccagtagc
    13621 agtatataaa gtgggtttca tggtaaccac agcgccagtg tcaacgtttt atcatcatcg
    13681 gtacgctcgt ttaccgctgg gctcgacaaa aactgcttta acaattcagg taataaatcg
    13741 agttgttcct gcgttgctgc cgccagtcca ttgaaaaacg gggtaaagaa aggcacatgc
    13801 ggagaattat ccagcatatt taacgccagt cgctgaatac catccgtaaa tgctgcaact
    13861 ttatgcgcac gctcagtgct ggtgaaagtt tccagtctgg aaacgacatc ttcatcggta
    13921 atgaaatgcg tcatattggc atattcaccc accatcggcg taagcggtaa ttgcagaccg
    13981 tgaccaaaat ccaccaccac gccgccatcg ccaatctgca taatcaaggt gccgttagcc
    14041 gatgaaatca atcccagaaa cgtgcaggca aaatcgcgaa ctgctaattc tttagcttcc
    14101 gcctcggcaa aaagtcgctg acgaacggtc agcacaatat ccgtggcgag aatatcattg
    14161 agtccaaatt caccgtgctg cactttttgc gacacataag ccatcgcttc attgatggca
    14221 agcatcgcgc cttcgccacc ctgcgagaca ctgcctgcgc catcagcaag gaacatcacc
    14281 agtaatggct gctggtcatt tagccaggca acctgcatct ggcaggcatc ctgacaaggt
    14341 aaatcagcgc taatgtgcga cgttccaaca gcggatgcat aaaccagacg ccagctcaca
    14401 ctgacgtcca gccttttggc gcttccagca caacttccgt tcccggcgtg gagcgggaaa
    14461 ccgaacgcag cgaactggat aaccagctaa acagttcacg gaattgtaat ccctgcagtg
    14521 gcaaaggctg acgaacgctg atttgtgcca gagtcttaat atccgccccc tgaacaccga
    14581 tggaaaagaa ggcaaacttc ttatcttctt ccccctgaaa cactttgttg gcggcagcct
    14641 gccactcatc ggttggtgca ccatcggtga tcaggaaaat ccacgggcga taatacgaaa
    14701 taccatttgc acgatactca cgtttccgct cctctaccat atccagggct ttggtaatgg
    14761 cggcacccat tggtgtatcc ccctgggcaa acaggatggg cgggaaaaaa ttagcggcgc
    14821 tggtaaaggg ttgttccaca tgcaccgggc cgaacgtcac aataccaagt tccactcttt
    14881 ttaaggccag cgggtcggca agcagctcat cgcgaaaggt aaccaatccg gcgttaagtt
    14941 cattaatggg tctaccgttc atcgaaccag aaacatccag cagcaaaatg caggggcaac
    15001 gtggttccgg gttactggca aaatcgctgg tggcaaacgt gatttgttcg ctcataagaa
    15061 atcctttctt agagaattat tgaggtccgg tatcataccg attgcaataa tgaaataaga
    15121 gcaaacgaag aaactttcag aattaggccc ggcggcgcag aaaattttgc tattcgcctc
    15181 gcaagaacgg agagccgtcg attgacacta agggcggagt gacataattt caggagtgag
    15241 ggttagggaa aggtttcccc ctccccctgg tgttcttagt aagcctggaa gctaatcact
    15301 aagagtatca ccagtatgat gacgtgcttc atcataaccc ttttcttatt taaggcccct
    15361 tcctcgggag gggctttccc gtttcagcat cccgctgaaa tcgtcggctt acctcctttc
    15421 gccatgcatg cagtctatcg ctgacttgcg gataaaatag tttccagtgt gattattgcg
    15481 taacgtctcg caaagaatat ttgctggcag gatcgcagac tgcaaagcct gcagattgac
    15541 aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
    15601 gtagtcttag taagccggga agcttatgac taagagcacc acgatgatga tccacttcat
    15661 catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc
    15721 cgctgaaatc gtcggcttac ctcctttcgc cctgaattca gtctatcgct gacttgcgga
    15781 taaaatagtt tcctctgtga ttattgcgta acgtctcgca aagaatattt gctggcagga
    15841 tcgcagactg caaagcctgc ggattgacat tctgaacgtg aaggcatact ttcaggagtg
    15901 agggtagagc ggggtttccc ccgccctggt agtcttagta tgctgggtag ctaatcacta
    15961 agagcaccac gatgatgagt atcttcatca tgaccctttc cttatttatg gccccttcct
    16021 cgggaggggg ctttcccgtt tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc
    16081 atgcaagcag tctatcgcta acgcgtagat aaaatagttt cctgtgttat tactggatgc
    16141 gtgctcgcaa atgtgcccgc cattcagacg attcccgata gtgtttcata attcctccat
    16201 ttttctccct tattggctgg ctacactagt atcattccgc gaaacgtttc aggaagagaa
    16261 actcttaacg atgaaaggca gttataaatc ccgttgggta atcgtaatcg tggtagttat
    16321 cgccgccatc gccgcattct ggttctggca aggccgcaat gactcccaga gtgcagcccc
    16381 aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt cgccgtggta tgcgttccgg
    16441 cccattagcc ccggttcagg cggcgaccgc cgtagaacag gcagttccgc gttacctcac
    16501 cgggcttggc accattaccg ctgctaatac tgttacggtg cgtagccgcg tggacggtca
    16561 actgatggcg ttacatttcc aggaaggcca gcaggtcaaa gctggcgatt tactggcaga
    16621 aattgacccc agccagttca aagttgcatt agcacaagcc cagggccaac tggcaaaaga
    16681 taaagccacg cttaccaacg cccgccgtga tctggcgcgt tatcagcaac tggcaaaaac
    16741 caatctcgtc tcccgccagg agctggatgc ccaacaggcg ctggtcagtg aaaccgaagg
    16801 caccattaag gctgatgaag caagcgtcgc cagcgcgcag ctgcaactcg actggagccg
    16861 tattaccgca ccagtcgatg gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat
    16921 ctccagtggt gataccaccg ggatcgtggt gatcacccag acgcatccta tcgatttgct
    16981 ctttaccctg ccggaaagcg atatcgctac cgtcgtgcag gcgcaaaaag ccggaaaacc
    17041 gctggtggta gaagcctggg atcgcaccaa ctcgaagaaa ttaagtgaag gcacgctgtt
    17101 aagtctcgat aaccaaatcg atgccactac cggtacgatt aaagtgaaag cacgctttaa
    17161 taatcaggat gatgcgctgt ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac
    17221 cgaacaaaac gccgtagtga tcccaacagc cgccctgcaa atgggcaatg aaggccattt
    17281 tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat ctggtgacgc cgggcattca
    17341 ggacagtcag aaagtggtga tccgcgcagg tatttctgcg ggcgatcgcg tggtgacaga
    17401 cggcattgat cgcctgaccg aaggggcgaa agtggaagtg gtggaagccc agagcgccac
    17461 cactccggaa gagaaagcca ccagccgcga atacgcgaaa aaaggagctc gctcctgatg
    17521 caagtgttac ccccgagcag cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtg
    17581 gccaccacgc tgctgatggt ggcgatctta ctcgccggga ttatcggtta tcgcgccctg
    17641 cccgtttcgg cgctgccgga agtggactat ccgaccattc aggtggtcac gctctaccca
    17701 ggtgcgagcc cggatgtcat gacctctgcc gttaccgcgc cgctcgaacg ccagttcggg
    17761 cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg gcggtgcgtc agttatcact
    17821 ttacagttcc agctaacatt accgctggat gtcgccgagc aggaagtgca ggccgcgatt
    17881 aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc cgccggttta cagcaaagtg
    17941 aacccggcag atccgccgat catgacgctc gccgtcacct caacagccat gccgatgacg
    18001 caagtggaag atatggtgga aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc
    18061 ggcctggtga cgctttccgg cggccagcgt ccggctgttc gcgtcaaact taacgctcag
    18121 gcgattgccg ccctcggcct gaccagcgaa accgtgcgca ccgccattac cggcgctaac
    18181 gttaactcgg ctaaaggtag ccttgacggc ccttcccgtg cggttacgct ttccgctaac
    18241 gaccagatgc aatccgccga agagtatcgc cagctaatca tcgcctacca gaacggcgcg
    18301 ccgattcgtc tgggcgatgt cgcaactgta gagcaaggtg cagaaaacag ctggctcggc
    18361 gcatgggcga ataaagaaca ggccattgtg atgaatgttc agcgccagcc cggtgctaac
    18421 attatctcca ccgccgacag catccggcag atgctgccac agctcaccga gagtctgccg
    18481 aaatcggtga aggtgacggt actttctgac cgtaccacca atatccgcgc atccgtcgat
    18541 gatactcagt ttgaattgat gatggctatc gcgctggtag tcatgattat ctacctgttt
    18601 ttgcgcaata ttccggcgac catcattccc ggcgttgcag tgccgctgtc gttaatcggc
    18661 accttcgcgg tcatggtgtt tctcgatttt tcaatcaata acctgacgct gatggcgtta
    18721 actatcgcca ccgggttcgt ggtcgatgac gccatcgtgg tgatcgaaaa tatttcccgc
    18781 tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca aaggcgcagg tgaaatcggc
    18841 tttaccatta tctcgctgac cttctcgctg attgcggtgt tgatcccact gctgtttatg
    18901 ggcgatatcg tcgggcgact gttccgcgaa tttgctatta ccctggcggt agcgatttta
    18961 atctcagccg tggtgtcgct aaccctgaca ccgatgatgt gcgcacggat gctcagccag
    19021 gagtcgttgc gtaaacagaa ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata
    19081 atcgccgcct atggtcgtgg actggcgaaa gtgctgaatc atccgtggct gaccttaagc
    19141 gtggcgctca gcacgctgct gcttagcgtt ctattgtggg tgttcattcc gaaaggtttc
    19201 ttcccggtac aggataacgg cattattcag ggcactttgc aggcaccgca atccagctcc
    19261 tttgccaata tggcccagcg gcaacgccag gtcgcggacg tgattttgca ggatccggca
    19321 gtgcaaagtc tgacctcatt tgttggcgtt gatggcacta acccgtcgct gaacagtgcg
    19381 cgtttgcaga tcaacctcaa accgttggat gaacgtgatg atcgggtgca aaaagtcatc
    19441 gcccgtctgc aaacggcggt ggataaagtg ccgggcgtcg atctcttcct gcaaccaacg
    19501 caggacctga ccatcgatac tcaggtcagc cgcacccagt accagtttac cttgcaggcc
    19561 acgtcactgg atgcgctcag tacctgggtg ccacagttga tggaaaaact ccagcagctg
    19621 ccacagattt ctgatgtctc cagcgactgg caggacaaag ggctggtggc gtatgtcaat
    19681 gttgatcgcg acagcgccag ccgtctgggg atcagcatgg cggatgtcga taacgccctg
    19741 tacaacgcgt ttggtcagcg actgatttcc actatttata ctcaggctaa ccagtatcgt
    19801 gtagtgctgg agcacaatac cgaaaatacc ccaggcctcg cggcgctgga taccattcgc
    19861 ctgaccagca gcgacggcgg cgtggtgccg ctaagctcaa ttgccaaaat tgagcagcgt
    19921 tttgcgccgc tttccatcaa ccatctggat cagttcccgg taacgaccat ctcctttaac
    19981 gtgccggata actattcgct gggcgatgcg gtgcaggcaa ttatggacac cgagaagacg
    20041 ctgaatctgc cggtggatat caccacgcaa ttccagggca gcaccctcgc cttccagtcg
    20101 gcgctgggca gcactgtctg gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc
    20161 ggcattctgt acgagagctt tattcacccg atcaccattc tctcgacgct acccacggca
    20221 ggtgttggcg ccctgctggc gttaatgatt gccggtagcg aactggatgt gattgcgatt
    20281 atcggcatta ttttgctgat cggtatcgtg aagaagaacg ccatcatgat gatcgacttc
    20341 gcgctggctg ctgagcgcga gcaaggcatg tcgccgcgcg atgcaatcta ccaggcttgt
    20401 ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg ctctgcttgg cgcgctgccg
    20461 ctgatgttga gtaccggggt cggcgcggaa ctacgtcgtc cgttaggtat cggcatggtc
    20521 ggcggcctga ttgtcagcca ggtgctgacg ctgttcacca cgccggtgat ttatctgctg
    20581 tttgaccgcc tggcattgtg gaccaaaagt cgctttgctc gccatgaaga ggaggcgtaa
    20641 gtgaagtttt ttgccctctt catttaccgc ccggtggcga cgattttact gtcggtcgcc
    20701 attaccctgt gcggcatact gggtttccgt atgctgccgg tcgccccgct gccgcaggtc
    20761 gattttccgg tgattatggt cagcgcctcg ctgcccggtg cgtcgccgga gacaatggcg
    20821 tcttccgttg ccacgccgct ggagcgctca cttgggcgca ttgccggggt cagtgaaatg
    20881 acctccagca gttcgctcgg cagcacgcgt attattttgc agtttgattt tgaccgggat
    20941 atcaacggtg cggcgcgtga tgtgcaggcg gcgatcaacg ccgcacaaag tctgctgccc
    21001 agtgggatgc ccagccgccc gacttatcgc aaagcgaacc cgtcggatgc gccaattatg
    21061 atcctcacgc taacctccga tacttattcg cagggtgaac tgtacgattt cgcctcgacg
    21121 cagctggctc cgacgatttc gcaaatcgac ggtgtcggtg atgtcgatgt cggcggcagc
    21181 tcgctgcccg ccgtgcgcgt cgggctgaac ccgcaggcgc tgtttaatca gggcgtgtcg
    21241 ctggacgacg tacgcactgc catcagcaat gccaacgtgc gtaaaccgca gggggcgctg
    21301 gaagatggca ctcaccgctg gcagatccag accaacgatg aactgaaaac cgccgctgaa
    21361 tatcagccgc tgattattca ctacaacaac ggcggcgcgg ttcgtctggg cgatgtggcg
    21421 acggtgaccg actcagtgca ggatgtgcgc aacgccggga tgaccaacgc caaaccggct
    21481 attttactga tgatccgcaa attgccggaa gccaatatta tccagacggt agacagcatc
    21541 cgggcaaaat taccggagtt gcaggaaacc attccggcgg cgattgatct gcaaattgcc
    21601 caggatcgct cccccaccat tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc
    21661 tcggtggcgc tggtgattct ggtggtcttt ttattcctgc gctcgggtcg cgccactatt
    21721 attcccgccg ttgcggtgcc ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc
    21781 ggattcagtc tcaataacct ttcgttaatg gcgctcacca tcgctactgg tttcgtggtg
    21841 gatgacgcca tcgtggtgct ggaaaacatt gcacgtcatc tggaagcggg aatgaaaccg
    21901 ttgcaagccg cactgcaagg tactcgcgaa gtcggtttta cggtgctgtc gatgagtctg
    21961 tcgctggtgg cggtgttcct gccgctgctg ttgatgggcg gattgccggg ccgactgtta
    22021 cgcgagtttg ccgtgacgct ttctatcgcc attggtattt cgttgctggt ttctctgaca
    22081 ttaacgccaa tgatgtgtgg ctggatgctg aaagccagca agccgcgcga gcaaaagcga
    22141 ctgcgtggtt ttggtcgcat gttggtagcc ctgcaacaag gctatggtaa gtcactgaaa
    22201 tgggtgctca atcatacccg tctggtgggc atggtgctgc ttggcaccat tgctttgaat
    22261 atctggctat atatctcgat tccgaaaacc ttcttcccgg agcaggacac tggcgtgttg
    22321 atgggcggga tacaggcgga tcagagtatt tcgtttcagg cgatgcgcgg taagttgcag
    22381 gatttcatga aaattatccg tgacgatccg gcagtggata atgtcaccgg ctttaccggc
    22441 ggttcgcgag tgaacagcgg gatgatgttt atcaccctca agccacgcga cgaacgcagc
    22501 gaaacagcgc agcaaattat tgaccgtcta cgcgtgaagc tggcgaaaga accgggggcg
    22561 aatctgttcc tgatggcggt acaggatatt cgcgttggcg gacgtcagtc gaacgccagc
    22621 taccagtaca cgttgttatc cgacgacctg gcggcactgc gcgaatggga gccgaaaatc
    22681 cgcaaaaaac tggcgacatt gccggaactg gcggacgtga actccgatca gcaggataac
    22741 ggcgcggaga tgaatctggt ttacgatcgc gacaccatgg cacggctggg aatcgacgta
    22801 caggccgcca acagcctgtt aaataacgcc ttcggtcagc ggcaaatctc gaccatttac
    22861 cagccgatga accagtataa agtggtgatg gaagtggatc cgcgctatac ccaggacatc
    22921 agcgcgctgg aaaaaatgtt cgtaatcaat aacgaaggaa aagcgatccc gctgtcgtat
    22981 ttcgctaaat ggcaaccggc gaatgcccca ctatcggtga atcatcaggg attatcggca
    23041 gcctcgacca tctcgttcaa cctgccgacc ggaaaatcgc tctcggacgc cagtgcggcg
    23101 atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg tgcgcggcag ttttgccggc
    23161 acggcgcagg tgttccagga gacgatgaac tcacaggtga tcctgattat cgccgccatc
    23221 gccacggtgt atatcgtgct gggtatcctt tacgagagtt acgtacatcc gctgacgatt
    23281 ctctccaccc tgccctcggc gggcgttgga gcgctgctgg cgctggagct gttcaatgcc
    23341 ccgttcagcc taatcgccct gatagggatc atgctattaa tcggcattgt gaagaaaaac
    23401 gccattatga tggtcgattt tgcgcttgaa gcccaacggc acggtaacct gacgccgcag
    23461 gaagctattt tccaggcctg tctgctgcgt tttcgcccga ttatgatgac taccctggcg
    23521 gcgctgtttg gcgcgctgcc gctggtgttg tcgggcggtg acggctcgga gctgcggcaa
    23581 cccttgggga tcaccattgt cggcggtctg gtgatgagtc agctcctgac gctgtatacc
    23641 acgccggtgg tatatctctt tttcgaccgt ctgcggctgc gtttttcgcg taaatctaaa
    23701 caaacggtaa ccgagtaaat gacagatctt cccgacagca cccgctggca attgtggatt
    23761 gtggctttcg gcttctttat gcagtcgctg gacaccacca tcgtaaacac cgcccttccc
    23821 tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc acatggtcat tgtctcttat
    23881 gtgctgaccg tggcggtgat gctgcccgcc agcggctggc tggcggacaa agtcggcgtg
    23941 cgcaatattt tctttaccgc tatcgtgctg tttactctcg gttcactgtt ttgcgcgctt
    24001 tccggcacgc tgaacgaact gttgctggca cgcgcgttac agggcgttgg cggtgcgatg
    24061 atggtgccgg tcggcaggtt gacggtgatg aaaatcgtac cgcgcgagca atatatggcg
    24121 gcgatgacct ttgtcacgtt acccggtcag gtcggtccgc tgctcggtcc ggcgctcggc
    24181 ggtctgctgg tggagtacgc gtcgtggcac tggatctttt tgatcaacat tccggtgggg
    24241 attatcggtg cgatcgccac attgatgtta atgccgaact acaccatgca gacgcggcgc
    24301 tttgatcttt ccggcttttt attgctggcg gttggcatgg cggtattaac cctggcgctg
    24361 gacggcagta aaggtacagg tttatcgccg ctggcgatta ctggcctggt cgcagttggt
    24421 gtggtggcac tggtgcttta tctgctgcac gccagaaata acaaccgcgc tcttttcagt
    24481 ctgaaactgt tccgcactcg taccttttcg ctgggcctgg cggggagctt tgccggacgt
    24541 atcggtagcg gcatgttgcc ctttatgacg ccggttttcc tgcaaattgg ccttggcttc
    24601 tcaccgtttc atgccggact gatgatgatc ccgatggtgc ttggcagcat ggggatgaag
    24661 cggattgttg tacaggtggt gaatcgcttt ggttatcgtc gggtactggt agcgaccacg
    24721 ctgggtctgt cgctggtcac cctgttgttt atgactaccg ctctgttggg ctggtactac
    24781 gttttgccgt tcgtcctgtt tttacaaggg atggtcaact cgacccgttt ctcctccatg
    24841 aacaccctga cgctgaaaga tctcccggac aatctggcga gcagcggcaa cagtctgctg
    24901 tcgatgatta tgcaattgtc gatgagtatc ggcgtcacca tcgccgggct gttgctggga
    24961 ctttttggtt cgcagcatgt cagcgtcgac agcagcacca cacaaaccgt ctttatgtac
    25021 acctggctta gcatggcgtt tatcatcgcc cttccggcgt tcatctttgc cagagtgccg
    25081 aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa ggagcgcgca atgaagttct
    25141 ggcgacctgg tattaccggc aaactgtttc tggcgatttt cgccacctgc attgtcttgc
    25201 tgatcagtat gcactgggcg gtgcgtatca gttttgagcg tggctttatt gattacatca
    25261 agcatggtaa tgaacagcga ttacaactgt taagtgatgc gcttggcgag cagtatgcgc
    25321 agcatggcaa ctggcgcttc ctgcgcaaca atgatcgctt tgtctttcag atcctgcgtt
    25381 catttgaaca cgataattcg gaagataaac ccggcccggg tatgccacca cacggctggc
    25441 gtactcagtt ctgggtggtt gatcaaaaca acaaggtgct ggttggtccg cgagcgccga
    25501 ttgcacctga cggtacacgg cgacccattc tggtcaacgg tgcggaagtg ggcgcggtga
    25561 ttgcctcccc tgttgagcgg ctgacccgca atactgatat caatttcgat aaacaacagc
    25621 ggcaaaccag ctggctgatt gtcgccctgg caacgttact cgcggcactc gctacttttc
    25681 tgctggcgcg cggtttgctg gcaccggtaa aacgacttgt cgatggcacg cacaaactgg
    25741 cggcgggcga tttcactacc cgcgtaacgc ccaccagtga agatgaactg ggcaaactgg
    25801 cgcaagactt caaccagctc gccagcacgc tggagaaaaa ccagcaaatg cggcgcgatt
    25861 ttatggccga tatttctcac gaactgcgta cgccattagc ggtgctgcgc ggtgaactgg
    25921 aagccattca ggatggcgtg cgcaaattca cgccggagac ggtggcgtct ttacaggcgg
    25981 aggtcggtac actgaccaaa ctggttgacg atcttcatca gttgtcgatg tctgatgaag
    26041 gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc actgctggaa gtggcaggcg
    26101 gcgcatttcg cgaacgattt gccagccgtg gcctgaaact gcaattttcc ctgccagaca
    26161 gtatcaccgt atttggcgat cgcgaccgtt taatgcagtt attcaataac ttactggaaa
    26221 acagcctgcg ctacactgac agcggcggca gcctgaaaat ctctgccgag cagcacgaca
    26281 aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt cagtgacgat cagctacaaa
    26341 aattgtttga acgtttttat cgcaccgaag gttcccgcaa ccgcgccagc ggcggttccg
    26401 ggctggggct ggcgatttgc ctgaacattg ttgaagcaca taatggccgc attattgccg
    26461 cccattcgcc ttttggcggg gtaagcatta cagtagagtt accgctggaa cgggatttac
    26521 agagagaagt atgaccgagt taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga
    26581 agatgaaccg aagctgggac agttgctcat tgattatctg cgtgctgcga gctatgcgcc
    26641 gacgcttatc agccacggcg atcaggtact gtcgtacgtg cgccagacgc cgccggatct
    26701 gatcctgttg gatctgatgc tccctggcac cgatggcctg acgctgtgcc gggaaattcg
    26761 tcgtttttct gacattccta tcgtgatggt gacagcaaaa atcgaagaga tcgaccgcct
    26821 gctagggctg gagattggcg cagatgacta catttgcaag ccgtacagcc cacgggaagt
    26881 ggtagcgcgt gtcaaaacca ttttgcgccg ttgcaaaccg cagcgtgagt tgcagcaaca
    26941 ggatgctgaa agcccgttga ttatcgacga aggtcgtttt caggcttcat ggcgcggtaa
    27001 aatgcttgac ctgacgcctg cggaatttcg tctgctgaaa acgctctctc atgaaccagg
    27061 aaaagtgttc tcccgcgagc aattgctcaa tcatctttat gacgactacc gcgtagtaac
    27121 cgaccgtacc atcgacagcc acattaaaaa cctgcgccgc aagctggaat ctctcgacgc
    27181 cgaacagtca tttatccgcg ccgtttacgg cgtcggttac cgctgggaag ccgacgcctg
    27241 ccgcatcgtt tagttttagc gacattactt tgttagccgg agatggcatt cagaccagat
    27301 ccggcaacat tatcccacgc atggtcagca aactgacatt atctctccgc cctggcaggc
    27361 atcagaaaat cgctcatact ttaatcggta aacagcacct ttagatgctg ttttcgatac
    27421 gcaatttcaa tcaaggagtc attatggctg gttggtttga actcagtaag agcagtgata
    27481 gtcagttccg gtttgtgcta aaagcgggca atggtgagac gatcctcacc agcgagctgt
    27541 atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag
    27601 aagaacgcta tgagaaaaaa acggcaagca acggcaaatt ctatttcaat ctgaaagccg
    27661 ccaatcatca aattatcggc tccagccaga tgtatgccac cgcgcaatct cgtgaaaccg
    27721 gaattgcctc cgttaaagcc aatggcacaa gccagacggt gaaagacaat acgtaattac
    27781 gatgccgggc gtgggttgtt ggcgtccggc gctttaccag atccccccta cagcgctaca
    27841 atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa gcggatctga
    27901 cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg gcgggaacgc
    27961 tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc
    28021 gttactccct gcgtgtgcgc aacaacgaat tcaaccacga aaaccttcag ctcggcatca
    28081 atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca ccgcacaacg
    28141 ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc
    28201 tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct gaaatgccga
    28261 tccatctctc ggtacaggct aacgccgtga actgggcgac ggtgaaattc tggcagcaaa
    28321 tgggactgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt gaagagatcc
    28381 gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggcgcactg tgcatggcct
    28441 actccggtcg ctgcctgctc tctggctata tcaacaagcg cgatccgaac cagggtacct
    28501 gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat gacgttggca
    28561 acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg ctgggtatcg
    28621 gcgcgccaac cgacaaagtg tttatgatcg aagaagccca gcgtccgggc gagtatatga
    28681 ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg cgcgccatcg
    28741 cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa ggccgtacta
    28801 aatctttcta ctattgcgca cgcaccgcac aggtttatcg taaagctatc gatgatgccg
    28861 ctgcgggcaa accgttcgat accagcctgc tggaaactct ggaaggtctg gcgcatcgtg
    28921 gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac tacgaatacg
    28981 gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag cgcaaggggg
    29041 acctcgcggc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt gagctgatga
    29101 cgccgcaagg caacattaac tttacccttg agcacatgga aaacgccaaa ggcgaagcta
    29161 tgccggtagc accaggcgat ggttatactg tgtggctccc ggtgccgcag gatcttgagc
    29221 taaattacgc gctgctgatg cgtaatttct ccggggaaac tacgcgtaac ccccacggta
    29281 agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga tcacatacag
    29341 ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa aatgctagct
    29401 gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta ttttttatgc
    29461 ataagcccta tccctgataa ccgtcttcca ttgaccacat cgatagaatc tcccttcata
    29521 gcacgatgcc tttcacgtaa cggcatcgtg ctcgcacagg ttccggctaa gcacaaccag
    29581 aacgcgcatg tttgacgctt accaaaaaat attctcactc tccacatttg aatgtcagac
    29641 gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc atttttttgc
    29701 cctgagtaac acagaaatgg gctgcatcat tttttactat attttctgca ccagatattc
    29761 tgcccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa
    29821 tattactttt atatgcaaga ccaaatttac cgatacttgt ctcatcatta tgcacagcac
    29881 aaccagacat aagaaaaaca ctaattaatg atatagcagc tatctttttc atctcacctt
    29941 cccccattaa ataccaacga cactctctag tgtttaaata taataatggc atgattatta
    30001 taattgaata ggattataat aaatgttctg taaaacattt cctacataag taggaattac
    30061 ggacatggag gcccttccgg gtaactccat ggaggcttta atatattata ttgaagaagc
    30121 cactgtttag ttgaatatta ggtatatgct cttttttgaa atttatcggt ggctgccgtt
    30181 agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc catacttgaa
    30241 ctgactattt atcctccgac tttcatcctg ttctgactcc accttttctt ttctgctcta
    30301 cactatctat agaccaatca taaaggcaca tacgatcatg gcagaatttc ccgccagctt
    30361 actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa ttatgctgtt
    30421 gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggcg atgccgcacg
    30481 atatgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg gtggcgatgg
    30541 caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca tacccgcgct
    30601 gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga ttcctgaggc
    30661 actggataag gcgctgaaac tggcaattgc cggtaacgcc attgcgatag atatggcgca
    30721 ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga cgcgtattac
    30781 cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca tcattcatgg
    30841 cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg aaaactttca
    30901 ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg gtggcggtca
    30961 acaattgtgc ccgaacgcgt taattaacga tggcttgctg caactgcgca tttttaccgg
    31021 cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata acccgaatat
    31081 tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca cttttaatct
    31141 tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa atacttccgg cggcgttgcg
    31201 ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc tcttgaacca
    31261 gggagaatcc gtaatgccgg atgcggtgta aacaccttat ccggcataca gaacaatact
    31321 acaccgtaat taattttacc cgtgtcttct gcaatgccag tttatccaca tctgacacgg
    31381 catcatcggt aataataatg tcaatttttt cgattggcag tacctgatta aaaccgcgac
    31441 gattaaattt cgatgaatcg agaacggcaa tcactttatt tgcggcggtg accatcgccc
    31501 cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca ataccatcag
    31561 caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca atggaaccat
    31621 gcatcgagcg cgttttatgg cggaccgtgc caccacagac gaccagagta atgtctttat
    31681 tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata tttttggcgg
    31741 tcattaatcc ttcggcgata agcattgtcg tacttccgct gtcgagaata acggtcatcc
    31801 cttcgtggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg ctggcaagct
    31861 gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattacca gacattattt
    31921 tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag cgcaaatcgg
    31981 cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca agcacggttc
    32041 cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc atcgtcattt
    32101 acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat gatctgtttt
    32161 gcacaatacc gggccagcat cacgctggcc cgcggtttca gggaatgagc aacactttgc
    32221 ccggcatagc attacgagcg atgtcacgca ccgcctgggc gaagctttca aagcttccac
    32281 ggtgagcgat taatggctcc aggcttaact tacgttctgt cagcaaccgg ctcgccgttt
    32341 cccactcctg ccccggccaa gggctggagt agttcatcca actgccgata accgtcagct
    32401 ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga tgcaacgtgc
    32461 ccaccagtgc cagttgggca tgaggaccgg caatctctac cgccagttcg acagtttgcg
    32521 gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttcgcgtaaa acgctctgca
    32581 tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa gattttgcca
    32641 gtgccagttt ttctgagcta atgtcgatag ccgtcacact ctttgctccc agcgcgacag
    32701 cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg tttttattct
    32761 cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggctca ataaaagccc
    32821 catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta acgacaatat
    32881 attcggcaaa tccaccatca cgccgtgagc caataaaatc atattttgcg cactgggaat
    32941 aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca caggcaaccg
    33001 catcgccagg gtgtaaatca tcaacaccgg atcctaccgc atcaatatag ccgctaaatt
    33061 catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg ggtaaatcgg
    33121 aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt ttaatttcag
    33181 gaatgacgct ttctgcaacg cgcacgatac catcagtatc attcaccact gatttcataa
    33241 aaactcctaa ttgttaaggg ggataacctc cccctcaggt aaaaattatt ctgcgagaac
    33301 gactttctct tgtttaataa agccacgcgc tctacgccag gtcatgaaaa taccggtaag
    33361 ataaattgcg ccgataataa tgaaaccggg aatattttgc ggggagaaaa cctgaatcag
    33421 taaccaggta atgggagaac cgccctgatc cattgaagcc accatacccc cggcttttaa
    33481 cgccccagca ttagccgcca gttgggtgtg caaaccaata gtttgcgtcg cgatccacag
    33541 ggtgatgctc ataatgatga cacccgagat taaggtgcgg aacagatttc cacgatgcac
    33601 cgcgaccgcc atcgccacga agaagccgat agtggcaaga tcgccaaacg gcagtacctg
    33661 attaccagga acacaaacag caattaaaat ggtgagcgga ataaaaatca ggcttgccga
    33721 taccaccgcc gtatgcccca gcagtaatgc gggatccagg ccaatcagga actcctgacc
    33781 gccgaacttc gcctgtaaac gactacgggc ctgcttagcg atgggcgtta aaccatccat
    33841 gatgggttta atcacccgtg gcattagcag catcaccgcc gccgttttta ccgccagctg
    33901 caatacacct ttgacatcgt aacccgcgag gatgccgata atcagcccca tcacaaaacc
    33961 cacggtgaca ggctcgccaa acgggccaaa tttgcgctga atatcgtcgg cgctaaattt
    34021 aatacggtta acgcctggga ttttctcgat gatagcatcg accagcaccg caatcggccc
    34081 catatacgcc gacgtaccgt gcggaatagc aatgccttcc agctcaaaga aatttcgggt
    34141 atcgcgggca aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta ccacacctgc
    34201 catccctatc atccatgaac cggttgccag atgcagcaac gcgccggtga aggtcatatg
    34261 ccagatattc cagatatcaa catttaccac ccgcgtcata cgggtcagta acatcgccac
    34321 gttaaccaga atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc aggtcattgg
    34381 tgaagagccc ggccagccga catcgaccac atgcagattc aggtcgaaat tttccgccat
    34441 cgctttcgcc gccgggccaa tggaatccag cattaagcca atcacaaggc caatgccaac
    34501 aaagccaatc ccgatatgca gacccgcttt aaagcaatcg cctgccttca tgcctaaaat
    34561 tttagaaaaa ataatgatga caatcggcaa catcaccgta gggccgagat cgagaatata
    34621 acgcatgact tctgaaaaca taggtcaccc ctgtaagata gtcagaattt tattttgtaa
    34681 tgcttcgata ccgacaccag aaacaaaagg catgccgtga actaacggaa tatcgccaaa
    34741 actacgatcc accctggcag tggtgcatat caaatgcacg ccatccatat aggtttctat
    34801 ttcattaacc cgacactgga ttaattcaac aggaatatta tgactctgac acaactcttt
    34861 aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga caataatctt
    34921 gcgtttcata aatagtcctt tttattgtta cagagggatt tattcgtctg aatctaaaac
    34981 atactttgtg aagtattcct ttaactgagt ttcaggaaga gtgattagtg tctcaacgat
    35041 atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct gctgcggatt
    35101 ttccacaatc aacgcaataa ccaacgatac cgccacgtca ttatcatcat ccgcttgctg
    35161 aaaatgaact ttatttgttg gccttaacag ataaatggct gacgacttag catgaatcgc
    35221 ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag ggaattctgc
    35281 ttctctggca attaatgcct gtggccaggt atcatgaacc acacctttgg cgagcatctc
    35341 attaccgata tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc tacgaacaaa
    35401 caggttagtc atataccgtc cttattccgc acagccgtag cgataggcgc gcaaaacatc
    35461 ataaatttta tccataatca gctgatgcgg tattgctgat aattccccgg actgaatgcg
    35521 ttcaaattgt ttgggaagat actgactaat catgcccagt gggatgtcca cgccttgcag
    35581 attcaccatc atcgtttcaa cgctattttt aatccgacta tgcggccagt aataacgaat
    35641 acgatccgac aggctgtaac gaatatccag taatgaatcg ttaaaacccg tgcgataata
    35701 ttttttccag tattgcggtt cgtcgagcat cacttcttca attaccgcca ggcaaccgct
    35761 gcgattttcg ggggcgataa gttcctgctc aatttgcgcc agcgcaaata tcgcctcacg
    35821 taaagcaaag gttaatgcgg gaccgacttt caatattgca aagtgatcgc ggactaattc
    35881 ccaataagcc gtccgggtct ggtaatcggt agaatgtgct tcataaacca ttcgggtatt
    35941 ttctatccat tgcgccagcg cctgcgcttc ctgcggctga taatggataa tattgctgtg
    36001 atcaaattcc acacccggct gcaccacgat ggcaatcacg cgtgttaatg cctctgtcag
    36061 cccacgggca ataaaggcct tttgatgcgt acgtaaagta ttggcggcat cttcaacatg
    36121 ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc ggaaccggaa cttcggtgcc
    36181 aatgacatag ctcagttgct cacgctggca atctgtcgcc acactttccg cagcaaagca
    36241 aagcacagca gctcgttccg caaccgtttc tggtgctaac ggtatgggat cccccgcgca
    36301 ggacattgac gcatcaagat gaattttact gaagccagca cgaacatatg cctttaccag
    36361 ctcgacggat ttttccatcg ccgcatccac attttcttgc tgccagcagt ttggccccag
    36421 atggtcaccg ccgagaataa tacgctcgcg tgcaaatccg actttatcgg caatcgcaaa
    36481 aacaaattcg cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt gattgacctg
    36541 gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg ccagcgccgc
    36601 ttcgataacc aacggatggg cagaacagac tgaacatatg ccgatatgtt caccagcttt
    36661 atgccgggca attaacgttt tcatgttttt tccttgttaa atggcaggtg cgttatgccc
    36721 tgccctcgca gccacaatcg gcaatcactt tgctcaccac atcgcgcatt gcggatttag
    36781 ccgactgcaa ataatcccgg ggatcggtcg cttcggggtg ctcggtcagg taatttttta
    36841 acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt gattttgcat atccccagtt
    36901 tgatggtttg ctgaatatcc ttagtcgata accctgacgc gccatgcagc accagcggta
    36961 agttcaccca ctggcgaatg ttctccagtc gagaaaaatc aagcgccggt gcgctggcat
    37021 acataccatg agccgtgccg atggcgaccg ccagggaatc aattccggtt gcctcggcaa
    37081 attcacgcgc ctgagcgggg ttggtataaa aagcatcggc ttcattgact tgcacatcat
    37141 cttcctggcc gccaagttgc cccagctccg cttcgacgct gacatcaaag cgatggcaaa
    37201 aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc gaggcgtcaa
    37261 tcatgactga gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat ttcgtgtgat
    37321 ggtcgagatg aatcgccagt ggatggtgat aatgcttcgc catcgcgctg accagcgcca
    37381 acagattttc tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg atgaccggcg
    37441 catgcaggtt ggcagcggtt tctaccacca cttgcatcgt ttcgagattg tgaatattga
    37501 atgccggaac cgcgtaaccg ccgcgctgcg cgttgttcag catctgcttt gtcgatacca
    37561 cgtacatttt cacatcctgt cgattgcttt cgatttcaaa acataatgaa atcatttgtt
    37621 tttaaatgtc gagataacga tcacaaaaac gacaatatga aaattaatag aggaatgaaa
    37681 ggcaaaaaaa acggcctccc ggagggaagc cgcaacaaag tgcgcgtggt tttatgcagg
    37741 atgcggcgta aacatatgtg gcagcgctgc gtaggcatga taagacgctt cagcgtcgca
    37801 tcaggcatag gttgccggat gcggcgtaaa cgccttatcc gtcctacggg gtagtgccgt
    37861 tcgtatcagg cgatagtaat tttgctatcg agataaacgt cctgcacggc gttaatcagt
    37921 ttcacgccgt cagccatcga tttcttgaac gccttacgtc caagaatcag ccccattccg
    37981 ccagcgcgtt tgttgataac cgcagtacgc actgcatcac tgaggtcagt ttcaccgcct
    38041 gcagcaccac cggagtttat caacccggcc cggcccatat agcagttagc taactgataa
    38101 cgcaccagat caatcgggtt ttcgctggtt aacttgctgt acacgcgatc gtcggtataa
    38161 ccgtaattaa ttgctttata gccgccgtta ttttccgcca ttttttgttt gacgatatct
    38221 gcacctatgg ttgccgccag atggttcgcc tgaccagtca ggtcggcgga gacatggtaa
    38281 tcaacgccat ctttcttaaa ggcggagtta cgcaaatagg cccacagcac tgtcaccatg
    38341 cccagctcgt gcgcacgttc aaaagccgca gaaatttctt caatctggcg acgtgactct
    38401 tccgagccaa aatagatagt cgcaccaacc gccaccgcgc ccatgttgaa ggcctgctcc
    38461 acgctggcat acagcgtttg atcgtaggta ttcgggtaac ttagcgtctc gttgtgatta
    38521 agttttacga gaaatggaat gcgatgcgca tagcgccgcg ataccgacgc caacacgccg
    38581 taagttgatg ccacacagtt acagcccgct tcgatcgcca gttcaacaat gtttttcggg
    38641 tcaaagtaga gcgggttagc agcaaatgaa gctccggcag agtgctcaac gccctggtca
    38701 accggcagaa tagaaagata tcctgtgcca gccagacgcc cggtgttgta caacgtctgc
    38761 atattacgta acaccgctgg cgggcgatta ttgtcaatca tcacgcggtc tacgtagtca
    38821 tgtccgggga gataaagctg gtcagaagga atagtcatac aacggtgctg taaaaggttg
    38881 tcggcgtctt tgccaagcaa ctgcgcaata tctgtcatta ctatgctccc gtaaattccg
    38941 attggatatc ggctatggat tgtcctggcc cgccttttgc gggcaatcat aatcctggtc
    39001 gttacgggcg agtttttcca tcttttggat gttttttcag cgttttctgt tggctcgatt
    39061 catcagaaaa aatgttagcg cggtcaagtt ttcaccgcaa aggtatttaa aaggtattat
    39121 taagtggtat tgtcatcgcg taccttacat tacctgtcat gaaggaatta aaagatgaaa
    39181 acaacaacaa agctgtcgtt catgatgttt gttgaatggt ttatctgggg cgcgtggttt
    39241 gtgccattgt ggctgtggtt aagtaaaagc ggttttagtg ccggagaaat tggctggtcg
    39301 tatgcctgca cagccattgc ggcgatcctg tcgccaattc tggttggctc catcactgac
    39361 cgctttttct cggcgcagaa agtgctggcg gtattgatgt tcactggtgc ggcgctgatg
    39421 tatttcgctg cgcaacagac cacttttgcc gggttcttcc cgttactgct ggcctactcg
    39481 ctaacctata tgccgaccat tgcgctgact aacagcatcg cttttgccaa cgtgccggat
    39541 gtggagcgtg atttcccgcg cattcgtgtg atgggcacta tcggctggat tgcctctggt
    39601 ctggcatgtg gtttcttgcc gcaaatgctg gggtatgccg atatctcacc gactaacatc
    39661 ccgctgctga ttaccgccgg aagttctgct ctgctcggtg tgtttgcgtt tttcctgccc
    39721 gacacgccgc caaaaagcac cggcaaaatg gacattaaag tcatgctcgg cctggatgcg
    39781 ctgatcctgc tgcgcgataa aaacttcctc gtctttttct tctgttcatt cctgtttgcg
    39841 atgccactgg cgttctatta catctttgcc aacggttatc tgaccgaagt tggcatgaaa
    39901 aacgccaccg gctggatgac gctcggccag ttctctgaaa tattctttat gctggcattg
    39961 ccgtttttca ccaaacgctt tggtatcaaa aaggtattat tgcttggtct ggtcaccgct
    40021 gcgatccgct atggcttctt tatttacggt agtgcggatg aatatttcac ctacgcgtta
    40081 ctgttcctcg gcattttgct gcacggcgta agttacgatt tttactacgt taccgcttac
    40141 atctatgtcg ataaaaaagc ccccgtgcat atgcgtaccg ctgcgcaggg actgatcacg
    40201 ctctgctgcc agggcttcgg cagtttgctc ggctatcgtc ttggcggtgt gatgatggaa
    40261 aagatgttcg cttatcagga accggtaaac ggactgactt tcaactggtc cgggatgtgg
    40321 acttttggcg cggtgatgat tgccattatc gccgtgctgt tcatgatctt tttccgcgaa
    40381 tccgacaacg aaattacggc tatcaaggtc gatgatcgcg atattgcgtt gacacaaggg
    40441 gaagttaaat gaaaacagaa cgtattctcg gtgctcttta tgggcaggcg ttaggggatg
    40501 cgatggggat gccctccgag ctttggccac gcagccgcgt taaagcacac tttggctgga
    40561 ttgaccgttt tctgcctgga ccaaaggaga acaacgcggc ctgttatttt aaccgcgccg
    40621 aattcaccga cgatacctcg atggcgctgt gtctggcgga tgcgttactg gaacgtgaag
    40681 gcaagatcga cccgaatctg attgggcgta atattctcga ctgggcgctg cgtttcgacg
    40741 cctttaacaa aaacgtacta ggtccgacct cgaagattgc gcttaacgcc attcgcgacg
    40801 gtaaacccgt tgctgaactg gaaaacaacg gcgtgaccaa cggcgcagcg atgcgcgtct
    40861 cgccattagg ttgtttgctt ccggcgcgtg atgttgattc ctttattaat gatgtggcgc
    40921 tggcgtcgag cccgacccat aaatccgatc tggcggttgc gggcgcggta gtcatcgcat
    40981 gggcgatttc tcgtgctatt gacggagaaa gctggtcagc gattgttgat tcattgcctt
    41041 caattgcgcg acatgcacag caaaaacgca ccaccacgtt cagcgcctca ctggcagcgc
    41101 ggctggagat tgcgctgaaa attgtgcgca atgccgacgg cactgaatcc gccagcgaac
    41161 agctttacca ggtcgttggc gcaggtacca gcactattga gtccgttccg tgcgccattg
    41221 cgctggttga actggcacaa accgacccga atcgctgcgc cgtcctgtgc gctaaccttg
    41281 gcggcgatac agacaccatc ggtgctatgg cgacggcaat ctgcggcgcg ttgcatggca
    41341 ttaacgctat cgatcctgcg ttaaaggcgg agttggatgc ggtaaatcag cttgatttca
    41401 accgctatgc cacagcgctg gcgaaatatc gtcaacaacg ggaggcggca tgagcggcgc
    41461 tcgattacac acgctgctgc ctgaattaac cacgcgtcag ccggttatgg tgattggcgc
    41521 ggcagtcatt gatgtgatcg ccgacgctta tgccctcccc tggcgtgggt gcgatatcga
    41581 actgaaacag cagagcgtta acgttggcgg ctgcgcactg aatattgccg tggcgttaaa
    41641 gcgcctcggc atcgaagcgg gtaacgcctt gccgctcggt cagggcgtgt gggcggagat
    41701 tattcgcaac cggatggcaa aagagggctt aatcagcctg atcgataacg ccgaaggtga
    41761 taacggctgg tgtctggcgc tggttgagcc ggatggcgaa cgcactttta tgtcattcag
    41821 cggtgtggaa aatcagtgga atcgccagtg gctggcgcga ttaaccgttg cgcctggcag
    41881 cctgctctat ttttccggtt atcaactggc ctcgccctgc ggcgaattat tagtggaatg
    41941 gctggaaaag ctgcaagacg tgacgccgtt tatcgatttt ggcccacgta ttggcgatat
    42001 cccggaggca ttactggcgc ggatcatggc ctgtcgacct ttagtgtcgc tcaatcgtca
    42061 ggaggctgag attgccgccg aacgttttgc tttatccgca gagataacaa cacttggcaa
    42121 gcaatggcag gagaaatttg ccacgccgtt gatcattcgt ctcgataaag agggcgcatg
    42181 gtatttcagc aacgacgctt ctggctgcat tccggcattt cccacgcaag ttgtagacac
    42241 cattggggcg ggcgacagtc atgcaggtgg cgtacttgcc gggttggcct ctggtctgcc
    42301 actggcggat gccgtattac tgggcaatgc agtggcgtcg tgggttgtcg ggcatcgggg
    42361 cggtgattgt gcgccaacgc gcgaggaact actcctcgca cacaaaaacg tatagatcgc
    42421 tgcgacagtg gctaatgctg tactcaatag gccgttgctg ttggtcaagc gccacttgct
    42481 tgatcaccag caccggtatt ttgctatcta actgaatatg tgactgaaac tcggcatctg
    42541 gcatccgggc gctaacgcgg gaacgtgtac gttgtgggta aatgtgctgg ctgcggaaat
    42601 agtcataaag agaaattccg atggcatcaa catcatgaat taaatgcgcc ggaacccacg
    42661 attcctcaat cgataccgct tcttcatcga catagcgaat acgcttaagc aaaaagacat
    42721 cacttcccgc ttcgaccgcc agctgctgcg cgacttcttc gggacatttc acgacacgct
    42781 tgttaaccca tagcgtatcg ggctttttcc cgcgcaatac cacctgctga gaaaaacctc
    42841 gcgcttcttt caacgagtat tcgaagatgt tgttgatctg cgtgccgtaa ccgcgcgaac
    42901 gcgtcaccac accctcttct tccagcgcct gcatcgcctt gcgcaccgta atgcgtgaaa
    42961 cgccggttaa ctgacttaaa tcacgctcac cgggcaaaat attgccatgc tccagcaccc
    43021 cgctgcgcac ggcatttttt accgtttcgg caaacttaat ataaagcggc gtgttatccg
    43081 ccgctaaaat acgctcgttc agttgggcaa tcagctgggt atgcgcttgt tccatttgtc
    43141 tttttcctgc cagggagtct ggtgggagta tatcactgct tcttatttaa ggcgtttctt
    43201 taatcccatc aacgaatgcc tgcaactcct cctctgtgcc attaaacaca ttaaaatcca
    43261 ccgaaccatt aatgccatct acctgcccac ggtcggaatg ctgccagaaa cgccaggcca
    43321 tgccgtcatt gtccgggcga cgttgatagt agtgagccac ccaccacgga tattcattga
    43381 aatagcccgc cagattggtg tgataaaaaa cggctcctga gtaaataacc ggcttttttc
    43441 ccgtactttt ttcgaccatt tttagccact gacttacccg cttgcgtaat tcttttgccg
    43501 ataattttcc ccgttcttct acgtccagca cggcagggaa atcgccttgt gagaaatcca
    43561 ccgtttgcag aaataatcgc gcctgaacag gcgctgctac cgacggggag aaataatgat
    43621 acgccccgcg cagcaggcca ttttcgcggc ttagttgcca gttacgcgaa aaataggggt
    43681 ccaccagctt ttcgccttcc gtcgccttga taaaggcaaa ttgtaagcgg ataccgttat
    43741 cgcgcatttt tgccacccgc tgccagtcga tccgctcctg ccagcgcgaa acatctattc
    43801 cgtgaatggt gtaactggca ggaatatgaa tagcgaaaga tttaaccggg cgataaccgt
    43861 agaagtggat gtagtctgta atctgaactg ctgtcgaata gaaaaaattc accgtctgac
    43921 gcggataaat tgcaacgatg gaaattagcc caagagcgca taatagagcg gtgagttttt
    43981 ttcgactggt aattcttagc tgcattggtt tcatccctga atgtcagtgc caaaggctga
    44041 caataaccaa agcagctata gtacggtgct caccaccagg cgtggaagtg gtgaaccgga
    44101 ccaataccgt gaccaacttc cagcgtgtcg gcctgggcta acgccgatga aagccagctt
    44161 tttgcctcct gtacggtgtc agcccagttt gtatggcgcg ggcgtagtgc agccaacgcc
    44221 gcagagagtg tacaaccagt gccgtgggtg tttttggtca taatgcgcgg tgcggtaaac
    44281 cgttgttcac cctcgcgggt aaacagccag tccgggcttt gctcatcatc cagatggcca
    44341 cctttcatta gcactgcgcc acagcccatc gccaacagcg ctcgcccttg ttccagcatt
    44401 tcctgttcgg tgcgcgcgtg tggcgcgtcg agcaaggcag cagcttcggg caagtttggc
    44461 gttattaatg aaacctgtgg caataatcga ctgcgtagcg tggcaactgc cgaaggtgaa
    44521 agcagtgggt cgccactttt tgccagcata acggtgtcga gtaccacgtt ttggatctga
    44581 taacgttgca accgttctgc caccgcttca acaatatcgg tttccgccaa cataccgatt
    44641 ttagtggtat cgattcgcac atcgctgaac accgaatcga gctgggcggc gacaaaatca
    44701 ggctcaatgc gatacaccga ctgtacgcca cgggtatttt gcgccaccag tgcagtaata
    44761 actgagcaac cataagcgcc aagtgccgag aaggttttaa gatcggcctg aatccccgca
    44821 ccaccactcg gatcagtacc ggcaatcgtc agagcgttaa ttcgtttcat gcctgcacct
    44881 cctgcgtcaa ttgccagagc gcatcaagga aatgtggaac aaaactgcct ggcccctcgc
    44941 ttctggcgac tgcgcgttct ccggcttgtt tcatccagtg acaggcagat gcgacatttt
    45001 ccagcatatc gcctggtaac gcacaacagg cagcgacaac cgccgataat gcacagccag
    45061 ttcctaccac tttggtcatt aacggatcgc caccgtgaat gccaacggcg cgatggccat
    45121 cggtaacata atccacctcg ccagtgacca cgacgattgc gccagtttcc cgtgccagtg
    45181 tttgtgcagc gggtatcgcg ttaactgcgg cgtcagtggt atccactccc cgtccaccat
    45241 tagcaacgcc agctaatgcc atgatttccg aagcattacc acgtatcgct gccggtttaa
    45301 aagataaaag ttcatgacaa aaatggcggc gataatcgag cgcaccaacc gctactggat
    45361 caagcgtcca gggtgtttga gagctttttg cttgctcaac ggcagcacgc atcgcctgtg
    45421 cgcgtggctg cgtcagtgtg ccaacgttaa tcaacaaggc actggcgata gccgcaaact
    45481 gactggcctc ttcggtttcg ataaccatcg ctggcgatgc accgagcgcc agcaaggtat
    45541 tggcagtaaa ggtttgcacc acatcgttgg tcatgcagtg cacaagaggg gaatgttggt
    45601 gaaaaaggtg taacgtgtgc gcagattgcg cggaactcag caggtcgact tgcatagttt
    45661 gctcctgcca taacgtgaag aagcaatgac ctggtggtcc gtgacttccc tacgctggca
    45721 ttatccagat caggtgatac gggtatttct cagccttcac gcagaagggc accccgagtc
    45781 gtttggttga atctctatat tttgtgccag cttaggggaa gaggcttgca gatgcaagct
    45841 cgcctccatt cattacgtct tcactgtgta ccccatatca gtaatgaaaa agaaacaatt
    45901 cacacccatc attcaggtgg taaaaactgt agactatctt aattagctaa tttatattat
    45961 ttatctaaat ttgataggtg agataaaaat gacagaaacc cagatctacg agaacattaa
    46021 gcaagccatt agcagcgcac cacgaaatag ccaaacgatg gaaatgcacc tccagatgat
    46081 caaatacgct gaccatctga aaaacgtcac ggcaaaagag ttttgcgaag gtgtaggact
    46141 taaagccagc tttgctacag agttcagcaa aatgagaaat cttaccgagc gactcaaagc
    46201 cgccggactt gataccacga agttataaca aacgaaatca tcaatatatt actttcgttt
    46261 tccagggttc cctgtatatg tacctatgat cataaaaacc agttacc
//
LOCUS       DAZFTN010000019        46013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFTN010000019 DAZFTN010000000
VERSION     DAZFTN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2790)
                     /gene="rhsB"
                     /locus_tag="ACHKV2_001303"
     CDS             complement(<1..2790)
                     /gene="rhsB"
                     /locus_tag="ACHKV2_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO6939224.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYDRD
                     YTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPDPE
                     LHPDSTLSMWPDNRIA"
     gene            complement(2950..3162)
                     /gene="rpmE"
                     /locus_tag="ACHKV2_001304"
     CDS             complement(2950..3162)
                     /gene="rpmE"
                     /locus_tag="ACHKV2_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6939225.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            3365..5563
                     /gene="priA"
                     /locus_tag="ACHKV2_001305"
     CDS             3365..5563
                     /gene="priA"
                     /locus_tag="ACHKV2_001305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO6939226.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            5719..6744
                     /gene="cytR"
                     /locus_tag="ACHKV2_001306"
     CDS             5719..6744
                     /gene="cytR"
                     /locus_tag="ACHKV2_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO6939227.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            6836..7795
                     /gene="ftsN"
                     /locus_tag="ACHKV2_001307"
     CDS             6836..7795
                     /gene="ftsN"
                     /locus_tag="ACHKV2_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO6939228.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            7888..8418
                     /gene="hslV"
                     /locus_tag="ACHKV2_001308"
     CDS             7888..8418
                     /gene="hslV"
                     /locus_tag="ACHKV2_001308"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO6939229.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            8428..9759
                     /gene="hslU"
                     /locus_tag="ACHKV2_001309"
     CDS             8428..9759
                     /gene="hslU"
                     /locus_tag="ACHKV2_001309"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO6939230.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            9826..10752
                     /gene="menA"
                     /locus_tag="ACHKV2_001310"
     CDS             9826..10752
                     /gene="menA"
                     /locus_tag="ACHKV2_001310"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO6939231.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            10845..11330
                     /gene="rraA"
                     /locus_tag="ACHKV2_001311"
     CDS             10845..11330
                     /gene="rraA"
                     /locus_tag="ACHKV2_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO6939232.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            11390..12064
                     /locus_tag="ACHKV2_001312"
     CDS             11390..12064
                     /locus_tag="ACHKV2_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939233.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            12187..12813
                     /locus_tag="ACHKV2_001313"
     CDS             12187..12813
                     /locus_tag="ACHKV2_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939234.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(12852..13097)
                     /gene="zapB"
                     /locus_tag="ACHKV2_001314"
     CDS             complement(12852..13097)
                     /gene="zapB"
                     /locus_tag="ACHKV2_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO6939235.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            13523..14368
                     /gene="glpF"
                     /locus_tag="ACHKV2_001315"
     CDS             13523..14368
                     /gene="glpF"
                     /locus_tag="ACHKV2_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO6939236.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            14391..15899
                     /gene="glpK"
                     /locus_tag="ACHKV2_001316"
     CDS             14391..15899
                     /gene="glpK"
                     /locus_tag="ACHKV2_001316"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO6939237.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            16129..17139
                     /gene="glpX"
                     /locus_tag="ACHKV2_001317"
     CDS             16129..17139
                     /gene="glpX"
                     /locus_tag="ACHKV2_001317"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6939238.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            17236..17982
                     /gene="fpr"
                     /locus_tag="ACHKV2_001318"
     CDS             17236..17982
                     /gene="fpr"
                     /locus_tag="ACHKV2_001318"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO6939239.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(17987..18415)
                     /gene="uspD"
                     /locus_tag="ACHKV2_001319"
     CDS             complement(17987..18415)
                     /gene="uspD"
                     /locus_tag="ACHKV2_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO6939240.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(18442..18741)
                     /gene="yiiS"
                     /locus_tag="ACHKV2_001320"
     CDS             complement(18442..18741)
                     /gene="yiiS"
                     /locus_tag="ACHKV2_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO6939241.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(18953..19393)
                     /gene="yiiR"
                     /locus_tag="ACHKV2_001321"
     CDS             complement(18953..19393)
                     /gene="yiiR"
                     /locus_tag="ACHKV2_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6939242.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            19494..20093
                     /gene="yiiQ"
                     /locus_tag="ACHKV2_001322"
     CDS             19494..20093
                     /gene="yiiQ"
                     /locus_tag="ACHKV2_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO6939243.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            20201..20968
                     /gene="tpiA"
                     /locus_tag="ACHKV2_001323"
     CDS             20201..20968
                     /gene="tpiA"
                     /locus_tag="ACHKV2_001323"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO6939244.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(21023..21778)
                     /gene="cdh"
                     /locus_tag="ACHKV2_001324"
     CDS             complement(21023..21778)
                     /gene="cdh"
                     /locus_tag="ACHKV2_001324"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO6939245.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(21885..22874)
                     /gene="sbp"
                     /locus_tag="ACHKV2_001325"
     CDS             complement(21885..22874)
                     /gene="sbp"
                     /locus_tag="ACHKV2_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO6939246.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(23193..24155)
                     /gene="pfkA"
                     /locus_tag="ACHKV2_001326"
     CDS             complement(23193..24155)
                     /gene="pfkA"
                     /locus_tag="ACHKV2_001326"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO6939247.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(24336..25238)
                     /gene="fieF"
                     /locus_tag="ACHKV2_001327"
     CDS             complement(24336..25238)
                     /gene="fieF"
                     /locus_tag="ACHKV2_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO6939248.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(25387..25887)
                     /gene="cpxP"
                     /locus_tag="ACHKV2_001328"
     CDS             complement(25387..25887)
                     /gene="cpxP"
                     /locus_tag="ACHKV2_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO6939249.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            26037..26735
                     /gene="cpxR"
                     /locus_tag="ACHKV2_001329"
     CDS             26037..26735
                     /gene="cpxR"
                     /locus_tag="ACHKV2_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO6939250.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            26732..28105
                     /gene="cpxA"
                     /locus_tag="ACHKV2_001330"
     CDS             26732..28105
                     /gene="cpxA"
                     /locus_tag="ACHKV2_001330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO6939251.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(28211..28885)
                     /gene="yiiM"
                     /locus_tag="ACHKV2_001331"
     CDS             complement(28211..28885)
                     /gene="yiiM"
                     /locus_tag="ACHKV2_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO6939252.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(29034..30017)
                     /gene="kdgT"
                     /locus_tag="ACHKV2_001332"
     CDS             complement(29034..30017)
                     /gene="kdgT"
                     /locus_tag="ACHKV2_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO6939253.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(30277..30897)
                     /gene="sodA"
                     /locus_tag="ACHKV2_001333"
     CDS             complement(30277..30897)
                     /gene="sodA"
                     /locus_tag="ACHKV2_001333"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO6939254.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            31182..32216
                     /gene="rhaT"
                     /locus_tag="ACHKV2_001334"
                     /pseudo
     CDS             31182..32216
                     /gene="rhaT"
                     /locus_tag="ACHKV2_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(32213..33061)
                     /gene="rhaR"
                     /locus_tag="ACHKV2_001335"
     CDS             complement(32213..33061)
                     /gene="rhaR"
                     /locus_tag="ACHKV2_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO6939255.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(33135..33971)
                     /gene="rhaS"
                     /locus_tag="ACHKV2_001336"
     CDS             complement(33135..33971)
                     /gene="rhaS"
                     /locus_tag="ACHKV2_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO6939256.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            34259..35728
                     /gene="rhaB"
                     /locus_tag="ACHKV2_001337"
     CDS             34259..35728
                     /gene="rhaB"
                     /locus_tag="ACHKV2_001337"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO6939257.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            35725..36984
                     /gene="rhaA"
                     /locus_tag="ACHKV2_001338"
     CDS             35725..36984
                     /gene="rhaA"
                     /locus_tag="ACHKV2_001338"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO6939258.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            37159..37983
                     /gene="rhaD"
                     /locus_tag="ACHKV2_001339"
     CDS             37159..37983
                     /gene="rhaD"
                     /locus_tag="ACHKV2_001339"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO6939259.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            37993..38307
                     /gene="rhaM"
                     /locus_tag="ACHKV2_001340"
     CDS             37993..38307
                     /gene="rhaM"
                     /locus_tag="ACHKV2_001340"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO6939260.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(38348..39742)
                     /locus_tag="ACHKV2_001341"
     CDS             complement(38348..39742)
                     /locus_tag="ACHKV2_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6939261.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCCIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            40220..40666
                     /gene="frvA"
                     /locus_tag="ACHKV2_001342"
     CDS             40220..40666
                     /gene="frvA"
                     /locus_tag="ACHKV2_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO6939262.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            40677..42116
                     /gene="frvB"
                     /locus_tag="ACHKV2_001343"
     CDS             40677..42116
                     /gene="frvB"
                     /locus_tag="ACHKV2_001343"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO6939263.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            42106..43176
                     /gene="frvX"
                     /locus_tag="ACHKV2_001344"
     CDS             42106..43176
                     /gene="frvX"
                     /locus_tag="ACHKV2_001344"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6939264.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            43176..44924
                     /gene="frvR"
                     /locus_tag="ACHKV2_001345"
     CDS             43176..44924
                     /gene="frvR"
                     /locus_tag="ACHKV2_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO6939265.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(44974..>46013)
                     /gene="yiiG"
                     /locus_tag="ACHKV2_001346"
     CDS             complement(44974..>46013)
                     /gene="yiiG"
                     /locus_tag="ACHKV2_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO6939266.1"
                     /translation="SSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPEAKPTE
                     APVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQ
                     GPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREY
                     NEMVDDYNSLR"
BASE COUNT        11089 a        11936 c        11839 g        11149 t
ORIGIN      
        1 ggcgatacgg ttatccggcc acatgctgag ggtgctgtcc gggtgcagct ccgggtcggg
       61 caggcggtta cctgccgggt ccgtggcata cgggatgcgg atatccagat tcgctgcggt
      121 ggtgtgaacg ccggtcagcc tgccggagtc gctgtagctg taactccggg tctggcgcgg
      181 gctgctgatg cggatgagtt cgccgttgtc gttccaggtg taatcgcggt catactgcag
      241 gctgttcagg tgctggctct gtaactgccc ggcaggggta taagcggtgg tgagttcata
      301 acggccgaat ctgcgcagcg ttttccggtg caggcggtcg cgggtgaaat ccaccagcgg
      361 tgtgtcgccg agcttcatgc ctgccagcca gccgctgcca taggtcagcc attccacggc
      421 gggcaggctg tccggtatac agcggttcgc cagcccctgc gcgttgtaag cgtgtctggt
      481 ctcatgctgc cagagcagtg cttccgtctg cgggtgatgc accgtctgac gctcaccggt
      541 cagccggcct ttctcatcat acccgtaatg caccgtcacc cggtgccctt cgctgatatg
      601 gctgatgtct gtcagccagc cgcgttcgtc atactgccag cgctctgcgg tttcaccctt
      661 cacggtgcgg tgcgtgaggc ggtctgcttc gtcatagtgc cagtgggtga ccagcccctc
      721 atcctcgctg cggataagtt tgccggtcag gtcgtggtga taacgctgtg tgcggccgtc
      781 aaagccggtt tcctgtatca gccggtcgag tacatcgtaa cggaaggtgg tgtggctgcc
      841 gttttcactg gtcaggcgga tgacccgtcc ggcagcatcg tattccatac tgcgcgtcag
      901 accgccctgc gtggtacaga tggcttttcc ccacgcatcg tactgtgtcc cgtttctgct
      961 gccgtccggg gcaatgacgg tggtcaggtc accggcggcg ttgtattcat accgcgtttc
     1021 atggccctgc gtgtctttca cggcaattaa ctgtccacgg ctgtcgtatg cgcggtactg
     1081 actcagccct tcctcgcggt gcaccgccgt catctgcccg aagcggtcat ggtcataacg
     1141 ggttacataa ccggaacagt cggtgaagct cagcaactga ccgtaacggc tccacgtcat
     1201 ggttttccgg ctgccggtgg catcttccgt tgcgcagggt aagtcactgt gtggattatc
     1261 ataacggtag cgggtgatat cgccgtcagg ggcagtttcc tgaatcagac ggccccattc
     1321 atcatattcc cggcgtattt ccagcccgtc aggcccggtg gctgacgtta actggctgtg
     1381 gtggttatag taaaacgccg atgccctgcc atccggcgtg gtgatgcgcg tgatgaggcc
     1441 cgtcaccaca tccgggctgt attctgttgt cctgcctgcg gcatccgtct gtgccctgag
     1501 cctgcccacc gcgtcaaact gactctgcgt gacgctgccg tccgcgtgtt cctttttcac
     1561 cacccgcttc agcccgcctt caccctgcgt gtgcaggact tcacggcggt tcaggctgtc
     1621 ggtgatggtg atgcggtctt tctcatactg atacgtgtag cttaagcctg ccgggtttag
     1681 ctgttctgtc acccgcccgt cgctgtcgta acggtaacgg atttccggcc ggcccgtgtg
     1741 acggtgcgcc accatccggc cccggtattt atcatcgtaa gtaaagctgc gcacctgttt
     1801 gccactacgg tcatacacca ccgccagttc gccgcgcggc gtccagccat agcgcaccag
     1861 cggcgcggca ggtaaattct ccgggtattc cgggtcgtgc gtcagccaca cggcagacag
     1921 acggatgccg ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga
     1981 cggttccgtc ccgccggaaa tggcctgctg ccgggcttct tctgcccgct gcgcctgcgt
     2041 ggtcagtacc agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct
     2101 gaattcaccg gcggcttcgc ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc
     2161 ggtcagtacc cggtacggcg gcagcggcgc aggcagcacc tcatccgctt ccggcacccg
     2221 ctcacaccag ccgagcagcc accacggccc ctgcggactg tttgtcgcca gataacgatg
     2281 cggacttaag cggagttctt ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc
     2341 atccagtttc gccacgccgc cgcgcaccag ccagagtgac tcgctgcggc tgtaaccgtc
     2401 ctcaccggga aacaggtgct caaaatacag gcttctgccg ccgttatcac tgagtatcag
     2461 tgtgttatcg cgcagctgta agcggatatc cgcaggcatt ttccagccgg ggccgaggct
     2521 ccccaccggc gcgggcgttt ttgtccggta actgctgtag gtgcgggaga ggatgaacgg
     2581 cagcgggccg ggcagggcga tgtcggtttc accgggaagg acctttgcac cgagcagggg
     2641 attgaccgga tggccggacg tcactccgcc ggggcacacc gaacaggcca cgccggtggg
     2701 ggcaccaatg cgcaccccgg ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc
     2761 gccctgacgc gccgccggtt ttccgctcat cgtaaaatcc ttttgaaata aataagaacc
     2821 agaagcctag cgccagtcaa caacaggaaa tgataatgag ttaacacccc ggaatttttc
     2881 agaatttata aaaggcaaat tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
     2941 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
     3001 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
     3061 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
     3121 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
     3181 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
     3241 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc atatgcaaac
     3301 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
     3361 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
     3421 cctgccagaa ggcatggcgg ttaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
     3481 gcaggagcgc atcggggttg tggtgtcagt tagcgacgtc agtgagctgc cgcttaacga
     3541 gctaaaagcg gtagtcgaag tgctggatag tgagccggtg tttactcact ccgtctggcg
     3601 attgctgctc tgggcggcgg attactatca tcatccgatt ggcgatgtgc tgtttcatgc
     3661 cttgccgatt ttactgcgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
     3721 tgccactgaa caaggccatg cggtggatct gaacagcctg aaacgttcgc caaagcaaca
     3781 acaggcactg gcggcattgc ggcaaggcaa aatctggcgc gaccaggttg ccacgcttga
     3841 atttaatgat gccgcgttac aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
     3901 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgaac ggttgcgatt
     3961 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcggata ccttctctgc
     4021 ttggctgctg gcgggcgtta caggttccgg taaaacagag gtttatttga gcgtactgga
     4081 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
     4141 gcaaactatc gcccgttttc gtgaacgttt taatgccccc gtagaagttc tgcattccgg
     4201 cctgaacgac agcgagcgtc tttcggcgtg gttgaaagcg aaaaatggtg aggcggcgat
     4261 tgtcatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggtg tgattgtcat
     4321 tgatgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
     4381 cctggcggtt tatcgtgcgc acagcgagca aatcccgatt attcttggct ccgcaacacc
     4441 cgcactggaa acgttatgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
     4501 tcgggcgggg aatgcgcgtc cggcaattca acatgtgctg gatttaaaag gtcagaaggt
     4561 gcaggcgggg ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccgacaa
     4621 ccaggttatc ctctttctta accgccgtgg ctttgcgcct gccttgctgt gccacgactg
     4681 cggctggatt gccgaatgtc cacgttgcga tcactactac acgctgcatc aggcgcaaca
     4741 gcatttgcgc tgtcaccact gtgacagcca acgcccggtg ccgcgccagt gcccttcctg
     4801 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgctcgc
     4861 gccgttattc cctggtgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
     4921 agcgctggaa caacaactgg cggaagtgca tcgcggcggc gcgaggatat tgattggcac
     4981 gcaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtagcgt tactggatgt
     5041 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgttttgccc agctatacac
     5101 ccaggtagca ggccgtgccg ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca
     5161 tcatccggaa cacccgctat tgcaaacgtt gctctataaa ggctacgacg cttttgccga
     5221 acaggcactg gctgagcggc gaatgatgca actgccgccg tggaccagcc atgtgattgt
     5281 gcgtgcggaa gatcataaca atcagcacgc gccattgttc ctgcaacaac tgcgtaatct
     5341 gatcctctcc agcccgctgg cagacgacaa attgtgggtt ctcggtccgg ttccggcact
     5401 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
     5461 gcgcttgcaa cacatcatta acggtacgct ggcactcatc aatacaatac cggattcccg
     5521 taaggtgaaa tgggtgctgg atgttgatcc gattgagggt taaaccgctc acgatgcgag
     5581 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
     5641 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
     5701 gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
     5761 gttgccctca aggcaaaagt ctctacagcg accgtctccc gagcattaat gaatcccgat
     5821 aaagtctccc aagccacccg taatcgggtt gaaaaagcgg cccgtgaagt gggttattta
     5881 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
     5941 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
     6001 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
     6061 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
     6121 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
     6181 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
     6241 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
     6301 gagatgccgc tgtgtcacta ccgcctgcaa ggttacgttc aggcgctgcg tcgctgcggc
     6361 attatggttg atccgcaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
     6421 gcgatgcagc agctgcttga tcttccacaa ccgcctactg ctgtcttctg ccatagcgat
     6481 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
     6541 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
     6601 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
     6661 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
     6721 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
     6781 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
     6841 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
     6901 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
     6961 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
     7021 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
     7081 ctggcgctac atcaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
     7141 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
     7201 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgaag ttccgtggaa
     7261 tgaacagacg ccggaacagc gtcagcaaac gctacagcgc caacgtcagg cgcagcaatt
     7321 agcggaacag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
     7381 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc
     7441 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
     7501 gccgcagcaa gctgcgccgg ttactcgtgc cgctgacgcg ccaaaaccga cggcggagaa
     7561 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
     7621 gacggtacgt gctcagctgg cgttcgaagg ctttgactca aaaattacca ccaacaatgg
     7681 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
     7741 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
     7801 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
     7861 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
     7921 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
     7981 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
     8041 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
     8101 ctggtcaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gttgcgcaaa
     8161 cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt
     8221 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
     8281 gctgcggcgc gtgcactgtt agaaaatact gaacttagtg ctcgtgaaat tgctgaaaag
     8341 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
     8401 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
     8461 gaactggata agcacatcat cggccaggac aacgccaagc gttccgtggc gattgctctg
     8521 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
     8581 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
     8641 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aattcaccga agtgggctac
     8701 gtcggtaagg aagtggattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
     8761 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
     8821 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agaccgaaca gcagcaggaa
     8881 ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
     8941 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccaggc
     9001 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg tcagaagcaa
     9061 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
     9121 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
     9181 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
     9241 gtttctcgtg aaggcgttca gcgagacctg ctgccgctgg tagaaggttg caccgtttcc
     9301 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
     9361 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc gactgccaat ccgcgttgaa
     9421 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
     9481 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
     9541 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
     9601 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
     9661 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
     9721 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
     9781 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
     9841 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
     9901 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcattttga tccgctggtc
     9961 gccctgctgg cactgattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    10021 tacggcgatg cggtaaaagg cagcgataaa cctgaccgta tcggaccgct acgcggcatg
    10081 caaaaagggg tcattaccca gcaagagatg aaacgggcgc tcattattac cgtcgtgctc
    10141 atctgtctct ccgggctggc actggttgca gtggcgtgcc atacgctggc cgattttgtc
    10201 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    10261 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggtta
    10321 agcgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    10381 gcgaccgcat gcggcctgct ggcaacggca gtactgaaca ttaataacct gcgtgatatc
    10441 aatagcgacc gcgaaaacgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    10501 cgtcgttatc atgcctgcct gctgatgggc tctctggtat gtctggcgct gtttaatctc
    10561 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccgtt gctggtgaag
    10621 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccgat gctggaacgc
    10681 actgtcaagg gagcgttact gactaacctg ctgtttgttg tagggatatt cctaagccag
    10741 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agaccacata
    10801 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    10861 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    10921 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    10981 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    11041 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    11101 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    11161 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    11221 aaagcgatgt ccgcgtcaat tttggcggtg ttaccttctt ctccggcgac catctttatg
    11281 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaataa gtatctgcca
    11341 tgcgttaaaa ggggaattaa attccccttt tttatacatg gacgtattca tgcagaatat
    11401 tattctttta tttatatatt tattacttat agccgttaac catttccgct acaaatttct
    11461 tttatctggc aacgcgggtg aaatgatttt atattttgct aacgtatttt ttaacccact
    11521 tgccttatca tttattatcg ccacaatcat ctgtataacg ataaaaaaaa gaagcagccg
    11581 cagctttatt cgcggcactt gttggttaat gggattattt cttatctata gcagctatac
    11641 ggtgtgggaa cgacacagca tatgggatta tactttcccg gagccaggta tcaccgtcac
    11701 ggttccgagc aaacagtggg ttgccaatat agtaaaacaa ggaccaaccc tggtgacccg
    11761 ggatgcccat gctattttag ctattgttgc cttctcacaa aatgaactcg gtgtgcattc
    11821 aatcgaggaa cttaccgcga ctcaaaacgg aactgcagaa atgcatgttt gtgatgttca
    11881 gggatttgcc tgcgcttatc aggaaggagt gcaaacaatg agtgatggca aagtaagaca
    11941 tgctattttc atgactctac tggataacac taatttgata caactggtcg cagctatcga
    12001 cccagattat ttggacgaat atcaagaaga agtaatgaaa atgatgcttt ctgctcgtca
    12061 atgatctgat gtgcatgtta ataataatgg attgatatga gatacgttat cttattaata
    12121 ctatggataa taactgtttt tcttagtcat cttcagcaca gtaacttgct tactggagat
    12181 ccagggatgg ttggcgagta tatcggttca gtacttcttg gaccgttatt acttcccgtg
    12241 cttgtgtcag gtattctgtg cacattcact aaaaaaacgc gtaacttcgc cagttttact
    12301 cgcgggtgct gttgggtatt gggagtcctg cttttatcca acgtcggcaa tacgtttcgt
    12361 ctgttcaccc cgtggcacta tacgtttgag aaggcagcta tttccgtgac tgtcccaaac
    12421 cgacactgga atacggtttc tattagtact gataaaacca ttgatataag aagtgaagat
    12481 aatagcgtct ttatttctgc tttccgcctg cccgcaggac gtagcgccga tgactcgctg
    12541 gaagagttaa agaagatgca gcgagacaat ctcaaggatc aatacaatga agaaaccttc
    12601 cagtttcatg attgtaatgc caaacatttc acctgtaagt atcaggatgt gttgataaat
    12661 tttgatggtc agcagaaaag aaccatcagt gtttatcttg aagacacccc cagggctgtg
    12721 gggatcattg cattaatgga gccagatacg gcagataaat atcgccaaca ggcgatggag
    12781 attatgttgt ctgctaaaaa tacagtgaag taaaaaataa ggcgccttgc ggcgcctctg
    12841 cgtggaagag attaaacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag
    12901 ccgttctgct gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg
    12961 gcattttgaa cttcctgcga caatgagttg tttttttctt tcagctcttc gatttccatc
    13021 tgcaacagag tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact
    13081 tctaatgaca ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaaatcctcg
    13141 tcccgattac cggtgacgcc ttaataaata cgagcgcact ttagttagct ccgattgtat
    13201 gaagccgcgc catcgctgtc cagcggcacg ccttgcagat tacggtttgc cacacttttc
    13261 atccttctcc tggtgacata atccacacca atcgaaaatg ttaataaatt tgttgcgcga
    13321 atgttctaac aaacatgcat catgtacaat cagatggaat aaatggcgcg ataacgctca
    13381 ttttatgacg aagcacacac attttaagtt cgatatttct cgtttttgct cgttaacgat
    13441 aagtttacag catgcctaca agcatcgtgg aggtccgtga ctttcacgca tacaacaaac
    13501 attaactctt caggatccga ttatgagtca aacatcaacc ttgaaaggcc agtgcattgc
    13561 tgaattcctc ggtaccgggt tgttgatttt tttcggtgtg ggttgcgttg cagcactaaa
    13621 agtcgctggt gcgtcttttg gtcagtggga aatcagtgtc atttggggac tgggggtggc
    13681 aatggccatc tacctgaccg caggggtttc cggcgcgcat cttaatcccg ctgttaccat
    13741 tgcattgtgg ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta tcgtttcaca
    13801 agttgccggc gctttctgtg ctgcggcttt agtttacggg ctttactaca atttattttt
    13861 cgacttcgag cagactcatc acattgttcg cggcagcgtt gaaagtgttg atctggcagg
    13921 cactttctct acttacccta atcctcatat caattttgtg caggctttcg cagttgagat
    13981 ggtgattacc gctattctga tggggctgat cctggcgtta acggacgatg gcaacggtgt
    14041 accacgcggc cctttggctc ccttgctgat tggtctactg attgcggtca ttggcgcatc
    14101 tatgggccca ttgacgggtt ttgccatgaa cccagcgcgt gacttcggtc cgaaagtctt
    14161 tgcctggctg gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca ttccttactt
    14221 cctggtgccg ctttttggcc ctatcgttgg cgcgattgta ggtgcatttg cctaccgcaa
    14281 actgattggt cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg agaccacaac
    14341 tccttcagaa caaaaagctt cgctgtaata tgactacggg acaattaaac atgactgaaa
    14401 aaaaatatat cgttgcgctc gaccagggca ccaccagctc ccgcgcggtc gtaatggatc
    14461 acgatgccaa tatcattagc gtgtcgcagc gcgaatttga gcaaatctac ccaaaaccag
    14521 gctgggtaga acacgaccca atggaaatct gggccaccca aagctccacg ctggtagaag
    14581 tgctggcgaa agccgatatc agttccgatc aaattgcagc tatcggtatt acgaaccagc
    14641 gtgaaaccac tattgtctgg gaaaaagaaa ccggcaagcc tatctataac gccattgtct
    14701 ggcagtgccg tcgtaccgca gaaatctgcg agcatttaaa acgtgacggt ttagaagatt
    14761 atatccgcag caataccggt ctggtgattg acccgtactt ttctggcacc aaagtgaagt
    14821 ggatcctcga ccatgtggaa ggctctcgcg agcgtgcgcg tcgtggtgaa ttgctgtttg
    14881 gtacggttga tacgtggctt atctggaaaa tgactcaggg ccgtgtccat gtgaccgatt
    14941 acaccaacgc ctctcgtacc atgttgttca acatccatac cctggactgg gacgacaaaa
    15001 tgctggaagt gctggatatt ccgcgcgaga tgctgccaga agtgcgtcgt tcttccgaag
    15061 tgtacggtca gactaacatt ggcggcaaag gcggcacgcg tattccaatc tccgggatcg
    15121 ccggtgacca gcaggccgcg ctgtttggtc agttgtgcgt gaaagaaggg atggcgaaga
    15181 acacctatgg cactggctgc tttatgctga tgaacactgg cgagaaagcg gtgaaatcag
    15241 aaaacggcct actgaccacc atcgcctgcg gcccgactgg cgaagtgaac tatgcgttgg
    15301 aaggtgcggt gtttatggca ggcgcatcca ttcagtggct gcgcgatgag atgaagttga
    15361 ttaacgacgc ctacgattcc gaatatttcg ccaccaaagt gcaaaacacc aatggtgtgt
    15421 atgtggttcc ggcatttacc gggctgggtg cgccgtactg ggacccgtat gcgcgcgggg
    15481 cgattttcgg tctgactcgt ggggtgaacg ctaaccacat tatacgcgcg acgctggagt
    15541 ctattgctta tcagacgcgt gacgtgctgg aagcgatgca ggccgactct ggtatccgtc
    15601 tgcacgccct gcgcgtggat ggtggcgcag tagcaaacaa tttcctgatg cagttccagt
    15661 ctgatattct cggcacccgc gttgagcgcc cggaagttcg cgaagtcacc gcattgggtg
    15721 cggcctatct cgcaggcctg gcggttggct tctggcagaa cctcgacgag ctgcaagaga
    15781 aagcggtgat tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt aattaccgtt
    15841 acgcaggctg gaaaaaagcg gtcaaacgcg cgatggcgtg ggaagaacac gacgagtaat
    15901 gtcaggcacc tgaatcgatt gcctgatgct gatcgcctga tgcgacgctt acgcgtctta
    15961 tcaggcctac gagccaatgc cattttgtgt aggcgggata aggcgttcac gccgcatccg
    16021 gcatcacgca gactctgaaa ccgtgcgggg gcaaccccgc acacatcaat aatcccaccc
    16081 ttcccctgtg ctacacttcg cgccattcct tactgcttag agtttgctat gagacgagaa
    16141 cttgccatcg aattttcccg cgtcaccgaa tctgcggcgc tggctggcta caaatggtta
    16201 ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa acgccatgcg tattatgctc
    16261 aaccaggtca acattgacgg caccatcgtc attggtgaag gtgaaatcga cgaagcaccg
    16321 atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg acgcggtaga tattgctgtt
    16381 gatccgattg aaggcacgcg catgacggcg atgggtcagg ctaactcgct ggcggtgctg
    16441 gcagtaggcg ataaaggctg tttcctcaac gcgccagata tgtatatgga gaagctgatc
    16501 gtcggaccgg gagccaaagg caccattgat ctgaacctgc cgctggcgga taacctgcgc
    16561 aatgtcgcgg cggcgctcgg caaaccgttg agcgaactga cggtaacgat tctggctaaa
    16621 ccacgccacg atgccgttat cgctgaaatg cagcaactcg gcgtacgcgt atttgctatt
    16681 ccggatggcg acgttgcggc ctcaattctc acctgtatgc cagacagcga agttgacgtg
    16741 ctgtacggaa ttggtggcgc gccggaaggc gtggtttctg cggcggtgat ccgcgcatta
    16801 gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg tcaaaggcga caacgaagag
    16861 aatcgtcgca ttggcgagca ggagctggca cgctgcaaag cgatgggcat cgaatccggt
    16921 aaagtattgc gtctgggcga tatggcgcgc agcgataacg tcatcttctc tgccaccggt
    16981 attaccaaag gcgatctgct ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa
    17041 acgctgctga tccgcggcaa atcacgcacc attcgccgca ttcagtctat ccactatctg
    17101 gatcgcaaag acccggaaat gcaggtgcac atcctctgat tgatttgatc gattgagcct
    17161 tccagtcctt cgggactgga atttttttgt tcggagaacg aagataaggc aattcaatca
    17221 aaacaggaga aaaacatggc tgattgggta acaggcaaag tcactaaagt gcagaactgg
    17281 accgacgccc tgtttagtct caccgttcac gcccctgtgc atccgtttac cgccgggcaa
    17341 tttaccaagc ttggccttga aatcgacggc gaacgcgtcc aacgcgccta ctcctatgtt
    17401 aactcgcccg ataatcccga tctggagttt tacctggtca ccgtccccga tggcaaatta
    17461 agcccacgat tggcggcact gaaaccgggc gatgaagtgc aggtggttag cgaagcagct
    17521 ggcttctttg ttctggatga agtaccagat tgcgaaacgc tatggatgct ggcaaccggt
    17581 acagcgattg gcccttattt atcgattctg caactaggta aagatttaga tcgcttcaaa
    17641 aatctggtcc tggttcacgc cgcacgttat gccgccgact taagctattt gccactgatg
    17701 caagaactgg aaaaacgcta cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa
    17761 acggcagcgg ggtcgctcac cggacggata ccggcattaa ttgaaagtgg tgaactggaa
    17821 agcgcgattg gcctgccgat gaataaagaa accagccatg tgatgctgtg cggcaatcca
    17881 cagatggtgc gcgatacgca acagttgctg aaagagaccc ggcagatgac gaaacattta
    17941 cgtcgccgac cgggtcacat gacagcggag cattactggt aagcggttac ttatcgataa
    18001 acggcacgat gagcaaatcc gcactcaact tattgatcat cccgcgatat gccggcatca
    18061 aacggttgat aaatgagtgg tgatgaccac agacaaggag gtcgcactgc tctttttgca
    18121 taatttccag cagtgtttcc ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc
    18181 attgaatatt tttcgtcagt ttatacagct tgttatccga cttattcttc aacaattgaa
    18241 gaatatcttc tgttgcaggg aagtagatac ccggatacaa ctcgcttaag ccatcatcaa
    18301 tatgaattaa cgtcaggtga gcgtcattat gtctggcgag ctccaaggct ttattcacca
    18361 gtaaggcatc ttcttcattc ccggaaattg ccacgccaat gtgtttataa gccatgttta
    18421 actccttcta aagcaactcc atcaagctaa cgaacgcagt gataactcaa aaataatcat
    18481 ctcggcctgg catgagaagg tgaaggccgc atcaagctca acttgcccct ccagatcctt
    18541 aatcttatag gccacgctgg ccccttcatc tgcagaggat gcagcggcgg caagctcttt
    18601 cagtttggtc ataaaggact ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact
    18661 ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc gcacatcctt tagtactgca
    18721 tttatctacg acatctttca tagggcacct ctgtatattc gcctctctgt cgattacgac
    18781 ttaagaataa tgcgccgtta agggaagaac aaacaagtaa tgatcttatg gattaccatt
    18841 ttttttgata ttgcgtaagg tgaagagttc agaaggattt tccgggtatt gtcatttgca
    18901 tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag atatattaaa ggttaattac
    18961 ttgatttatt gtcggcttta tacttcacat cctgagtatc tttaccatat ttattttcgc
    19021 cttgtgtgcc aacaaacgca ccaagatcga taagcatcat caccagaatc aacgtcggga
    19081 caaaacgccc taccgcccat tgccagacac ccggtaaaat cgcccagtta cccgccagta
    19141 gcatccacgc cacaatcatc agaaatgccc atgcgccgga acgcccgcga tcatgcaagc
    19201 gcttaacggt tactgccgct gttggccaga gcaagcacac aaggcaaaac gccgcggtct
    19261 gaatatcgag taaattttta cccgccagtg agaaaagcac cagcatgcct gcgaaccaca
    19321 ggcctatcca aatccagaaa tcacggcgtc caatacgccc tttaaatgag aataaccatt
    19381 gctgtatggt catgtaagtt ccttgatggt tgtcttttcc aggattctac ccttttgaca
    19441 agggcgacag atatcgtttt aatcggagcc agtcataaca aaaggtactg tcaatgaagc
    19501 cagggtgtac gctgtttttt ctcttatgtt ctgcattaac cgttacaaca acggcgcatg
    19561 cacaaacacc agatacggca acgaccgcgc cttatctgct ggctggagcc cctactttcg
    19621 atctctccat cagccagttt cgagaagact ttaacagcca gaatcccagc ctgccactga
    19681 acgaatttcg tgccatcgac agcagtcccg acaaagccaa tctcactcgt gctgccagta
    19741 aaattaacga gaacttgtat gcttctacag cgctggagcg cggtacctta aaaatcaaaa
    19801 gcattcaaat gacatggcta cccatccagg ggccagagca aaaagccgcg aaagcgaaag
    19861 ctcaggaata catggcagcg gtgatccgca cactcacccc attaatgacc aaaacacaaa
    19921 gccagaaaaa actgcagtcg ctactaacgg cggggaaaaa caaacgttat tacaccgaga
    19981 cagaaggtgc actgcgctat gttgtcgcgg acaacggcga aaaggggctg accttcgctg
    20041 ttgaaccgat taagctggcg ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag
    20101 caaagccttt gtgccgatga atctctatac tgtttcacag acctgctgcc ctgcggggcg
    20161 gccatcttcc tttattcgct tataagcgtg gagaattaaa atgcgacatc ctttagtgat
    20221 gggtaactgg aaactgaacg gcagccgcca catggttcac gagctggttt ctaacctgcg
    20281 taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat
    20341 cgatatggcg aagcgtgaag ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga
    20401 cctgaacctg tccggcgcat tcaccggtga aacctctgct gctatgctga aagacatcgg
    20461 cgcacagtac atcatcatcg gtcactctga acgtcgtact taccacaaag aatctgacga
    20521 actgatcgcg aaaaaattcg cggtgctgaa agagcagggc ctgactccgg ttctgtgcat
    20581 cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa gaagtttgcg cacgtcagat
    20641 cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga
    20701 acctgtatgg gcaatcggta ctggcaaatc tgcaactccg gctcaggcac aggctgttca
    20761 caaattcatt cgtgaccaca tcgctaaagt tgacgctaac atcgctgaac aagtgatcat
    20821 tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa ctgtttgctc agccagacat
    20881 cgacggcgcg ctggttggcg gtgcgtctct gaaagctgac gctttcgcag taatcgttaa
    20941 agctgcagaa gcggctaaac aggcttaagt ctgacaggtg ccggatttca tatccggcac
    21001 ttactttcct taactcttcg ccttaacgca aaatctcaca ctgatgatcc tgaatttcct
    21061 cggctgaagc acggttaagc gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt
    21121 tatcactctg ccgtaccatc gccagcccgt agcttcccat atgttcccgc gcctcaggta
    21181 cttcttctgc cagcatcata aatgggctgc gttgtaccag ttcgctttcc gttacccgac
    21241 gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt
    21301 tcgccagatt gttatccagc tgttcgcgca catcaggacg aatacaagag atatgaatat
    21361 gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg
    21421 gaaccggctg gccgtatttt ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga
    21481 agttcggcgt tgaaggatcg gtcaacaaag gactttcagt accgttaata cgatacgttg
    21541 gcatcaacag atattgcagt gggccattaa gatcttttaa aaccacgtat ccggcattgg
    21601 gtttgacttc cgcacatggc gaaggatttt gattttgctg ctgattgggc aaacattcct
    21661 caaggacaat cttacgtaat gtatccgact cttcaccggt taatttccag taaccaatac
    21721 cggcagccac aacggcgata actatcatca ccaaaaaaag aagacccgct tttttcatct
    21781 ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac
    21841 ccggtcgcga gaaaccaccg ggtttgataa ttatcctggg gaaatcagcg tttgctgatc
    21901 tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac
    21961 tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat tttcgtactt tttcgccacc
    22021 tcagcgtcgc gcggacggta gtagtttttc gcggcaattt cctgaccttc tggcgagtag
    22081 agatatttca ggtaggcttc cgccacctct ttagtacctt ttttctcgac cactttatcg
    22141 accaccgaca cggttggctc tgcgaggata gactcactcg gcgtgacgat ttcgaattta
    22201 tctttcccca gttcattcgc tgccagcaga gcttcgtttt cccaggaaat cagtacatcg
    22261 ccaattccgc gctcgacaaa agtgttagtg gagccgcgcg cgccagaatc cagaacttcg
    22321 acgtttttat acagtgcccg aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga
    22381 tgcagcgcgt agccccaggc tgccaggtag ttccagcgcg cgccaccaga gcttttcgga
    22441 ttaggcgtga tcaccgaaac acccagttta atcagatcgt tccagtcatg gatctgcttc
    22501 ggatttccct tacgtaccag gaaaacaatg gtggaagtgt acggtgcgga gttatccggc
    22561 agacgtttga tccactcttt atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag
    22621 gccagagcca gcgtgacaac atcagcttca ataccgttga ttaccgacgt tgcttgtttg
    22681 cctgaaccac cgtgtgactg acgaatcacc acgttatcgc cagtttgctg tttccagtgg
    22741 gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta
    22801 agaagctgaa tatcctttgc cataacgctg gttgccgcca gcaaaaatgt taaccctacg
    22861 ccccacttgt tcatcgcccg actctcttat gttgtgttgt gatgagcaaa gcgtgccaga
    22921 aggttaacca aacattaaag aataaaaaaa gattggctat aacttgcggg tatatgttgg
    22981 gggttaaaaa ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttatcgatgc
    23041 cggatgcggc gcgaacgcct tatccggcct acaaaagttt gtaaattcaa taaattgtag
    23101 aattcacgta ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc
    23161 aggctaaagg aatctgcctt tttccgaaat cattagtaca gttttttcgc gcagtccagc
    23221 cagtcgcctt tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc
    23281 agctgttcgt tctgtatacc tacgcaacga ccgccgtaac ctgccagcag cagatcgata
    23341 gcgtaagcgc ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc
    23401 tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc
    23461 gccagttcgt caacatcaca catatgttcg gtaatcgcca cgatcgcgtg ttttttacct
    23521 ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac ggctgaattc aacttccgga
    23581 accacaacga attcacagcc accggcaatg gccgcagcca gcgtcagatc gccacaataa
    23641 cggcccatca cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg
    23701 tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct
    23761 ttgatgtcgt tgtcgatagt gcccggcagg ccgatgcacg ggaagcccat ttcggtcaga
    23821 cgcattgcac ccatgtagga accgtcaccg ccgataacca ccagcgcatc gatcccacgt
    23881 tttttcaggt tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc
    23941 gcagaaccga ggaacgtacc gccacggttg atcatgtcag aaacgctgta acggtctagc
    24001 tgtaccatac ggtcttcata cagacccaga tagccgtcat aaatgcccat tacttccaga
    24061 ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca
    24121 ccgccgcttg tcaacacacc gattttctta atcatgacta cctctgaact ttggaatgca
    24181 aaataaaatc tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa
    24241 tagtataaca atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat
    24301 acacaaaatg cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc
    24361 tccctaggta cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc
    24421 cgtaaaatag cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag
    24481 tcttccattt ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga
    24541 tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttcc
    24601 tcatcaggta atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg
    24661 ctatataaaa tatagatgcc gattcccaat gcaaacagag catcggcgcg atgccagccg
    24721 taccaggaca accccagcgc cagcagaatt gcgccgttca tcataacatc agactggtaa
    24781 tgtagcatat cagcccgcac cgcctggctt tgcgtccggc gcaccaccca acgctgaaac
    24841 gagacaagga taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga
    24901 tctgtcatcg gtgttggaga tatcagatgt tgaatgcctg tcaaaaacag gaatagtgcc
    24961 gaaccggaga taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca
    25021 aacgagtgat tatcgtcggc aggttgcagg gaatatcgca ccaccaataa attcgtcaac
    25081 gacgcgccga tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta
    25141 taccaccatg caaaaatttt aatcagcaat agcagtgaag ccatcgccgt cgcagcaatc
    25201 gccgcccgac tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga
    25261 cgctagtata acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata
    25321 aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag
    25381 ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg
    25441 ccattgcgtc acgtcacgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc
    25501 ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg
    25561 acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa
    25621 attttctgcg gtgacaagac gatgcattgt ctccagttcg ctaacattaa caggaggctg
    25681 ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact
    25741 tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca
    25801 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc
    25861 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    25921 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    25981 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    26041 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    26101 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    26161 acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca
    26221 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    26281 gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt
    26341 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    26401 agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttagtactga
    26461 atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt
    26521 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa
    26581 gccaggaagt gctgggcaaa cgcctgacgc ctttcgaccg cgctatcgat atgcacattt
    26641 ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc
    26701 gtggtcgcgg ctacctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    26761 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    26821 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcagggtc tgatgattga
    26881 gcagcatgtt gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    26941 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac
    27001 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    27061 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    27121 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    27181 cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac tgctgattgt
    27241 caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    27301 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga
    27361 actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc agatggtcac
    27421 cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct ctcacgagct
    27481 gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagtggtga
    27541 aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca tgattaacga
    27601 cctgttggtg atgtcacgta atcagcaaaa aaacgcgctg gttagcgaga ccatcaaagc
    27661 caatcagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa
    27721 gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg tacggcaacc cgaacgccct
    27781 ggagagtgcg ctggaaaaca ttgttcgtaa tgccctgcgt tattcccata cgaagattga
    27841 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    27901 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcgcg
    27961 cgatcgtgaa tctggcggta caggtttggg actggcgatt gttgaaaccg ccattcagca
    28021 gcatcgtggc tgggtgaaag cagaagacag cccgctgggc ggtttacggc tggtgatttg
    28081 gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc
    28141 cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac
    28201 actccggggg ttattttccc cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc
    28261 ggcgcttttg catcgttcgc gtccagcttt tcgataaccc ggcagcggag agtaaacggt
    28321 gatactgatc gtcatcaaac ggcatatgcc atgcgatggc agcagcctcc tgcacggtca
    28381 catcgctgac acgggaaacc aactccagcg gcgcgtccgc agacactttc cctggcgcta
    28441 tcacgctata caaccatccc actttacccg tgttttgcat caactgcgct atatcgctga
    28501 tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg
    28561 cctctcccca gcggaaaata tcgccaatat aaacattact ttccgtgaga ccgtcggttg
    28621 agagattttc gccaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgcg
    28681 cccagtagag ataatgctca cgaggataat gacacagcgc tctgtctggt ccgccgtgaa
    28741 cctttttctc cgcctgctcg tcaccttcca gccccagctc cgttagcatc aactcaccat
    28801 caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg
    28861 tgtatacatc aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac
    28921 catatttatt ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca ggaactcaat
    28981 agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc
    29041 accgtaccta aaatttcgat tttcgccgct ctggctttga ctttacgtga ccagatagag
    29101 gtaagaattg gcaccagaat cgaagtcaca atgactgccg tcgctaccag cgaagttgct
    29161 gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc
    29221 gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat
    29281 ttatcggcga taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc
    29341 cccagcaaac ctgtctgggc aattacagtc aaatcaatgg tattgcctag tgcgaaggca
    29401 aagaatggaa tcagcgtctg caccgctttg ctgaaaaatt ctcgtaattc agggtcaagg
    29461 ttcccaaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca
    29521 aacgaggcaa tcccggcagt gcccagaata atcatcgtca tgagcggccc ggactccaac
    29581 gacatcaaca caaatgcccc agcttcttct tttgtgccgt actgctgcat gatggaagcg
    29641 taaagtccgc cgttggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca
    29701 aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc
    29761 cacgcgacgg caattttagt taccaccagc gtaccggatt tacgcagtac cgttcccgtc
    29821 gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta
    29881 ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg
    29941 tggcacagtg cgccaaggaa tagcgggacg agcatcatcc cccccgggat tttctcaatc
    30001 aagcgtttta tctgcatttc catcacctgt cggcatattt ttaagggctg atgcgtaatt
    30061 cctcaatgga gcatcagtgt tgatggaaac gaagatagac attcatttaa tcaataaaag
    30121 tgatcaaaac cacatcaact gaaacgctgt tttattttta tcggatcatt gtttttattt
    30181 tgaaattgat cacaaaaaaa ccaccgccat tggcgatggt tcatttttta agctgatatg
    30241 cggcgtaaac gcctcactgc agcagacggc aaatgattat tttttcgccg cgaaacgtgc
    30301 cgctgcttcg tcccagttca ccacgttcca gaactctttg atgtagtccg ggcggcggtt
    30361 ctggaatttc aggtagtaag catgctccca cacatccagg cccagaatcg ggaagccgga
    30421 tgcgccagaa atagcttcac ccatcagcgg ggagtcctgg ttagcagtag aaaccaccgc
    30481 cagtttatcg cctttcagca ccagccatgc ccagccggaa ccaaagcggg aagctgccgc
    30541 tttctcaaat tctgctttga agttatctac ggaaccgaaa tcacgttcga tagccgcttt
    30601 caggtcaccc tgcagggtgg tgcctttttt cagacctttc cagaacaggc tgtggttagc
    30661 gtgaccgcca gcgttgttgc gcagtacggt tttcttgtct gctggcagct ggtccagttt
    30721 ggtgatcaac tcttcaaccg gcaggttggc aaattctggc aggctttcca acgccgcgtt
    30781 ggcgttgttg acgtaggtct gatggtgttt ggtgtggtgg atttccatgg tctgcttatc
    30841 gaagtgcggt tccagggcat cgtaagcata cggcagggat ggcagggtat agctcatatt
    30901 catctccagt attgtcgggc ggccgattgt taatgccgcg taagcagttg gttcattata
    30961 gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcgtaag ggtatggttt
    31021 tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa
    31081 atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc acgattttta cactcatctc
    31141 gtcggagatg tgacgcgacg aaaaatgatg aggataagaa gatgagtaac gcgattacga
    31201 tggggatatt ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac gctccgttca
    31261 aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga
    31321 ttattctgcc gtaggccatc agcgccctgt tactaccgaa tttctgggcg tattacagct
    31381 cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta
    31441 atatcaacta cggcctgacc atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca
    31501 ttggcattac gttgattgtc ggtacgctga tgacgccaat tatcaacggc aatttcgatg
    31561 tgttgattaa caccgaaggc ggacgcatga cgttgctcgg cgttctggtg gcgctgattg
    31621 gcgtagggat tgtaactcga gccgggcagt tgaaagagcg caagatgggc attaaagccg
    31681 aagagttcaa tctgaaaaaa ggactggtgc tggcggtgat gtgcggcatt ttctctgccg
    31741 ggatgtcctt tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc gcactaggcg
    31801 tcgatccact gtatgtcgct ctgccaagct atgttgtcat catgggcggc ggcgcgatca
    31861 tcaacctcgg tttctgtttt attcgtctgg caaaagtgaa ggatttatcg ctaaaagccg
    31921 acttctcgct ggcaaaaccg ctgatcattc acaatgtgtt actctcagca ctgggcgggt
    31981 tgatgtggta tctgcaattc tttttctatg cctggggcca cgcccgcatt ccggcgcagt
    32041 atgactacat cagttggatg ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg
    32101 ggctggtgct gaaagagtgg aacaatgcag gacgccgtcc ggtaacggtg ttgagcctcg
    32161 gttgtgtggt gattattgtt gccgccaaca tcgttggcat gggcatggcg aattaatctt
    32221 tctgcgaatt gagatgacgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca
    32281 ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc
    32341 ggctatgctg gagaagatat tgcgcatgac acactctgac ctgccgcaga tattgattga
    32401 tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct
    32461 catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccaac cgggtaatca
    32521 gcttatccag caacgtttcg ctggatgttg gcggcaacga atcactggtg taacgatggc
    32581 gattcagcaa catcaccaac tgcccgaaca gcaactctgc catttcgtta gcaaacggca
    32641 catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgt gccatcccca
    32701 tgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgccccct
    32761 gccagtcaag attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat
    32821 cgttaacgga agcgtaggag tgtttatcgt cagcatgaat gtaaaagaga tcgccacggg
    32881 taatgcgata agggcgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca
    32941 gctcacaaaa atcatgtgta tgttcagcaa agacatcttg cggataacgg tcagccacag
    33001 cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca
    33061 ccgtggctac ctcggccaga gaacgaaatt gattattcgc aatatggcgt acaaatacgt
    33121 tgagaagatt cgcgttattg cagaaagcca tcccgtccct gacgaatatc acgcggtgac
    33181 cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccgcaatga
    33241 taggcgatgt cagtaacgct ggcctcgctg tgacgtagca gatgtcgggc tttcatcagt
    33301 cgcaggcgat tcaggtatcg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt
    33361 agcgtacgca gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca
    33421 aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc
    33481 tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg
    33541 gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc
    33601 acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagctct
    33661 tgtggcagca actgattcag tccggcgaga aactgaaatc gatccggcga gcgatacagc
    33721 acattagtca gacacagatt atcggtatgt tcatacagat gccgatcatg gtcgcgcaca
    33781 aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg
    33841 ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc
    33901 gggagccggg gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc cacactatgt
    33961 aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca
    34021 ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac
    34081 agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaattcagc aaattgtgaa
    34141 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
    34201 ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat
    34261 gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc
    34321 gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg
    34381 gctgcatagt cagaacggct atgtcacctg ggatgtggat agccttgaaa gtgccattcg
    34441 ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac
    34501 ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc
    34561 ttatcgcgat agccgcacca atggcctaat ggcgcaggca caacaacaac tcggcaaacg
    34621 cgatatttat caacgtagcg gcatccagtt tctgcccttc aatacgcttt atcagttgcg
    34681 tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc
    34741 ggattacttc agttatcgcc tgaccggcaa gatgaactgg gaatatacca acgccacgac
    34801 cacgcaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg
    34861 ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcatag gtcactggat
    34921 ttgcccgcag ggtaatgaga ttccagtggt cgccgttgcc agccatgata ccgccagcgc
    34981 ggttatcgcc tcgccgttaa acggttcacg tgctgcttat ctctcttctg gcacctggtc
    35041 attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcactgg cagccaacat
    35101 caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg
    35161 gctgcttcag cgagtgcttc aggagcggca aatcaacgat cttccggcgc ttatctccgc
    35221 gacacaggca cttccggctt gccgcttcat tatcaatccc aatgacgatc gctttattaa
    35281 tcctgagacg atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc aaccgatccc
    35341 ggaaagtgat gctgaactgg cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga
    35401 tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc tcgcaactgc atattgtcgg
    35461 cggaggctgc cagaacacgc tgctcaacca gctatgcgcc gatgcctgcg gtattcgggt
    35521 gatcgccggg cctgttgaag cctcgacgct cggcaatatc ggcatccagt taatgacgct
    35581 ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg cgaatctgac
    35641 cacctttacc cctaatcctg acagtgaaat tgcccactat gtggcgcgga ttcactctac
    35701 acgacagaca aaggagcttt gcgcatgacc actcaactgg aacaggcctg ggagctggcg
    35761 aaacagcgtt tcgcggcagt agggattgat gtcgaggagg cgctgcgcca acttgatcgt
    35821 ttacccgttt caatgcactg ctggcagggt gatgatgttt ccggttttga aaacccggaa
    35881 ggttcgctga ccggagggat tcaggctact ggcaattatc cgggcaaagc gcgtaatgcc
    35941 agtgagctac gtgccgatct ggaacaggct atgcggctga ttccggggcc aaaacggctt
    36001 aatttacatg ccatctatct ggaatcagac acgccagtct cgcgcgacca gatcaaacct
    36061 gagcacttca aaaactgggt tgaatgggcg aaagccaatc agctcggtct ggattttaac
    36121 ccctcctgtt tctcgcatcc gctaagcgcc gatggcttta cgctttccca tgccgacgac
    36181 agcattcgcc agttctggat tgatcactgc aaggccagcc gccgcgtttc ggcctatttt
    36241 ggtgagcaac tcggcacacc gtcggtgatg aacatctgga tcccggatgg catgaaagat
    36301 atcaccgttg accgtctcgc tccgcgccag cgtctgctgg cagctctgga tgaggtgatc
    36361 agcgagaagc tggatccggc gcaccatatc gacgccgttg agagcaaatt gtttggcatt
    36421 ggcgcagaga gctacacggt tggctccaat gagttttaca tggggtatgc caccagccgc
    36481 cagactgcgc tgtgcctgga cgccgggcat ttccacccga ctgaagtgat ttccgacaag
    36541 atttccgccg ccatgctgta tgtgccgcag ttgctgctgc acgtcagccg tccggttcgc
    36601 tgggacagcg atcacgtagt gctgctggat gatgaaaccc aggcgattgc cagtgagatt
    36661 gttcgtcacg atctgtttga ccgggtgcat atcggcctcg acttctttga tgcctctatc
    36721 aaccgtattg ctgcgtggat cattggtaca cgcaatatga aaaaagccct gctgcgtgcg
    36781 ttgctggaac ctaccgctga gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt
    36841 ctggcactgc tggaagagca gaaatcgttg ccgtggcagg cggtctggga aatgtattgc
    36901 caacgtcacg atacgccagc aggtagcgaa tggctggaga gcgtgcgggc atatgagaaa
    36961 gaaactttga gtcgccgcgg gtaaacactg ccggatgcgg cacaagtgcc ttatccggcc
    37021 tacaggccgg caatagttgt aggcctgata agacgcgaca gcgtcgcatc aggcattgat
    37081 tgccggatgc ggcacgagcg ccttatccgg cctacgggtc gtgcatctga caacaccgaa
    37141 tttacaggaa cacagaacat gcaaaacatt acccagtcct ggtttgtcca gggaatgatc
    37201 aaagccacca ccgatgcctg gctgaaaggc tgggacgagc gcaacggcgg caacctgacg
    37261 ctgcgcctgg atgacgccga tatcgcgctt tatcacgaca acttccaccc acaaccgcgc
    37321 tatattccgc tcagccaacc catgccttta ctggcaaata cgccgtttat tgtcaccggc
    37381 tccggcaaat tcttccgtaa cgtccagctc gatcctgcag ctaacttagg cgtcgtaaaa
    37441 gtcgacagcg atggcgcggg ctaccatatt ctctgggggt taaccaacga agccgttcca
    37501 acttctgaac ttccggctca ctttctctcc cactgcgagc gcattaaagc caccaacggt
    37561 aaagatcggg tgatcatgca ctgccacgcc accaacctga tcgccctcac ctatgtactt
    37621 gaaaacgaca ccgcggtctt cactcgccaa ctgtgggaag gcagcaccga gtgtctggtg
    37681 gtattcccgg atggcgttgg cattctgccg tggatggtac ccggcacgga cgaaatcggc
    37741 caggcaactg cccaagagat gcaaaaacat tcgctggtgt tgtggccctt ccacggtgtg
    37801 ttcggttccg gaccgacgct ggatgaaacc ttcggtttaa tcgacaccgc agaaaaatca
    37861 gcggaagtgt tagtgaaggt gtattcgatg ggcggcatga aacagactat cagccgtgaa
    37921 gagttgatag cgctcggcca gcgtttcggc gttacaccac tcgccagtgc gctggcgctg
    37981 taaggagcaa acatgatccg caaagccttt gtcatgcagg taaaccccga cgcccacgaa
    38041 gagtatcagc gtcggcataa tcccatctgg ccggaactgg aagcagtgtt gaaatctcac
    38101 ggtgcgcata actacgccat ttacctcgac aaagcgcgta atctgctgtt tgccacggta
    38161 gagattgaat ctgaagaacg gtggaatgcg gtagccagca ctgaaatctg ccaacgttgg
    38221 tggaaatata tgaccgatgt tatgcccgct aacccggata acagcccggt gagtagcgag
    38281 ctgcaagaag tgttttacct gccttaatga gttgcggtgc cggaaaatat ccagcaccgc
    38341 gacttcatca gaagaagtat ttaaagcgaa cgcgtgcgcc gtaacggtca tcagtatcat
    38401 caccgtgttt tttatcgccg tcgctatcca gaatggagta atacgtcccg aggtaaatat
    38461 taaagttctc catatccatc acgttagcga actgataaga cgcatgaatg gtgtgaatgg
    38521 tgtacgtccc ttcatcatca atgcagcagt cgttatcaca aactacaccg ctgaattcat
    38581 caatcttatt gtgcgcgtag atataaccga gctcgaaacg tttccacagc gcgttaatcc
    38641 cggcagtgaa atctttctca ttattagcat ctaaataggc ggtatttaga ttaaccacaa
    38701 tgccattgtc cggatccgtt ttcaggccgt tccaggtcat gctcatacca tagccggtac
    38761 gatcggactg atcgacaaaa ttacccttgc tatcggtata accataagca ttatttacca
    38821 cgttcgcttc catcgctgcg gaaacggtga attcttccgt cggcgaccag gcaattactg
    38881 ggcgcagata agcaacattt ttctgctgct ccatatcgcg tccatgataa ttaccgtcat
    38941 tatataaaga tgttccgtct tcaagtaacg tgtttaattc aaaataccag ttatcgagtt
    39001 gtttgctgac gaggaaatta ccgccagcgt tagagcgccc gcggccctct ttcatcatat
    39061 agatataacc gctgccatcg tcataaagat cgttcgcagt attaccggaa tgttcaacaa
    39121 aggtatcctg attcaatggg aacatatcgt aggcttcaaa acggcctact ttgaccttcc
    39181 agtcgttttc tttgccaaag aagaataccg catcatcgat atttactgaa ccgtgcatat
    39241 cacccagcgg ttgtgcggag aacccggcga aataaccgtt gtccatcttt cgcataccgt
    39301 caaaacctaa cagaatacga ccattgagat tccactgttc attagtggga tcgctattca
    39361 catcagcgtt ggtcattgcc agcaggccat gattactttc cgcatccata ttaaattcta
    39421 cgtcgccgta gatttttaaa tcgccataac ctgaaagcaa aagattaggt gaggtggcat
    39481 ttttctttgc agattccgcc gttgcctgtc catttaccgg cgtattgccc tgagcggcct
    39541 taatttcgcg gatctcagcg gcttgttgtg tctgcagctg tttaacctgg ctctctgctt
    39601 ttgccgcccg catttctgcc gcttccaggc gttcttccag caatgccatg cgttgttcca
    39661 gcattaaatg ctgtgttttt gccataccgg tactacatgc caataataaa ccgatggcta
    39721 aagataatgt cgattttttc attatttacg ttccctgaaa taaaggagac cctacgcttt
    39781 ccaaaaggaa tacgtatata gagaaatata aggtgtgcac tttcgaattt ttaatccaac
    39841 ttcagcaggc cgatggatag tatcccgccg cataatatga gtaaatccgc tgatttcctg
    39901 gaacgtgagg aatataacaa tagtgccgtt tttaactaaa tatcgtaaaa aatattagat
    39961 atgattcaag ctggctcata agccacaaat ataaataacg tttagacaaa ttatctgaga
    40021 catttaaaca ttcacaaaat cattaaataa taactcttta ttcacaagat ttattttacc
    40081 tactaatatg tgccttacgc cgcaaaattt atccttcact ctgctttata ttgtcacgac
    40141 ttcacgacat atccgcgctc atcacaaacc cgccccgcta ttacaattaa ctgaaatatt
    40201 ttttcatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc tgaatattca
    40261 gggcaatggc gcttattccg ttctgaagca gttggcgaca atggcgttac aaaacggttt
    40321 tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa tgcactccac
    40381 cggttttggt tctggtgtcg ccgtgccgca cggtaaaagc acctgcgtta aacaaccgtt
    40441 cgtgttattc gcccgcaaag cgcaggctat tgactggaaa gccagcgacg gcgaagacgt
    40501 caattgctgg atctgcctcg gcgtaccgca aagtggcgaa gaggatcagg tcaaaatcat
    40561 cggcacactg tgtcgcaaaa ttattcacca ggaatttatt catcaactgc aacagggcga
    40621 taccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa gtggcgatgg
    40681 agtcatcctt acgtattgtc gcgatcacca actgccccgc cgggatcgct cacacctaca
    40741 tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccatt aaagtggaaa
    40801 ctcaagggtc cagcggcgtt gaaaaccgct tatccagcga agagattgcc gctgccgatt
    40861 acgtgattct cgccaccggg cgtggcctga gcggtgacga tcgcgcacgg tttgccggga
    40921 aaaaagttta tgagattgcc atctcccagg cgctgaaaaa tatcgatcag attttcagtg
    40981 aattaccgac aaattcgcag ctttttgccg cagatagcgg cgtgaagcta ggcaaacagg
    41041 aagtacaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg gcgctgccgt
    41101 ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag ttcggtttgc
    41161 cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa tccatcggtt
    41221 accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg tcgattgccg
    41281 ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac aaagcgctgc
    41341 tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg gcgattggct
    41401 atttcgtctt ctggttccgt aaagtccgtc ttggcaaagc attacaaccg ctgctcggct
    41461 ccatgcttat cccattcgtt actctgctgg tttttggcgt gctcacttac tacgttatcg
    41521 ggccggtgat gtccgacctc atgggcgggc tactgcactt cctgaatacc attcctccat
    41581 caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat atgggcggcc
    41641 ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac atttatgact
    41701 ggtacgccat cgtcggcgtt gtccccgtcg cggcgggtct tgcgacgttc attgcgccca
    41761 aactgtttac ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc ggtgccacag
    41821 tcgcaaccga accggctatt ccttacgctc tggcagctcc gttgccgatg attaccgcca
    41881 atacgctggc gggcggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg
    41941 cacctggttt gggtatcttt gacccgctga tcggcctgat gtcgccggta ggttcgtttt
    42001 atctggtgct ggcgattggc ctcgcgctga acatttcatt cattatcgtc ctgaaaggat
    42061 tgtggttacg ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa cattgagtta
    42121 ctgcaacagt tgtgcgaagc cagcgccgtc agcggcgatg aacaggaagt tcgcgacatt
    42181 ctgataaaca cgctggaacc ttgcgttaat gagatcacct ttgatggtct gggcagcttt
    42241 gttgcccgta agggaaataa aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc
    42301 ggctttatgg tcacccacat cgacgagagc ggttttctgc gctttaccac cattggcggc
    42361 tggtggaatc agtcgatgct caaccaccgg gtaacaatac gcacacacaa gggagtgaaa
    42421 atccctggtg tgattggttc cgtagcgcct catgcgttaa cggaaaagca aaagcaacaa
    42481 ccgctgtcat ttgatgagat gttcattgat attggcgcga acagtcgcga agaagcgaaa
    42541 aaacgcggtg cggaaattgg cgattttatt agcccggaag ccaattttgc ctgctggggc
    42601 gaagataaaa tagtcggcaa ggcgctggat aatcgcatcg gctgcgcgat gatggctgag
    42661 ctactacaga cagtaaataa cccaggaatt acgctgtacg gcgtcggcag cgtggaagaa
    42721 gaagttgggc tacgcggggc acaaacctcg gctgaacaca ctaaaccgga tgtggtgatc
    42781 gtgctggata ccgccgtcgc gggcgatgtt ccgggcattg ataacattaa atacccgctg
    42841 aaactgggca acgggccggg gctgatgctg tttgacaagc gctacttacc caaccagaaa
    42901 ctggtggcgg cgttaaaaaa ctgtgccgca cataacggtt taccgctgca attttccacc
    42961 atgaaaaccg gagcgacgga tggcgggcgc tacaacgtaa tgggcggagg gcgtccggtt
    43021 gtcgcgctgt gtctgccaac tcgttatctg cacgctaaca gcggtatgat ttcaaaagcc
    43081 gattatgatg ctctgctcac gctgatacgg gattttctga cgaccttaac tgcggagaaa
    43141 gtcaacgcgt ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga
    43201 ttgtcgatct gctggagcaa cagccgcgca cgcctggcga gctggcgcaa cagactggcg
    43261 tttcaggcag gaccatcctg cgtgatattg actatctcaa ctttaccctt aacggcaaag
    43321 ctcgtatctc tgccagtggc agtgcgggct atcagttgga aatcttcgag cgccgcagct
    43381 tttttcagtt gctgcaaaag cacgataacg acgatcggct gctggcgctg ttattactga
    43441 atacctttac tccccgtgcg caactcgcct cggcgcttaa tttgccagaa acttgggtcg
    43501 cagagcgtct gccccggtta aaacagcgtt atgaacgcac ttgttgcctg gccagtcgtc
    43561 ccggtttggg ccatttcatt gatgaaacag aagagaaacg cgttatcttg ctggcgaact
    43621 tgctacgcaa agatccgttt ttaatacctt tggctggcgt aacacgagac aaccttcagc
    43681 atttatccac ggcctgcgac aaccaacacc gctggccgct catgcagggg gattatctct
    43741 ccagcctgat tctggcgatt tacgccctgc gtaatcaact gaccgatgag tggccgcaat
    43801 atcccggtga cgagataaaa caaatcgttg aacataacgg tctgtttctt ggtgataacg
    43861 ctgtaagaac cctgacaggt ttgatagaga aacagcatca gcaagcgcag gtaatttctg
    43921 ccgatcatgt gctggggttg ctgcaaaggg tgccaggtct cgcgtcattg aatattattg
    43981 atacgcagct ggttgagaac attaccggtc atttattacg ttgccttgcc gcaccagtgt
    44041 ggattgctga gcatcgccaa agcagcatga ataacctgaa agccgcctgg cctgcggcct
    44101 ttgatatgag tctgcacttt attacgctac tgcgtgaaca gctcgatatt ccccttttcg
    44161 acagcgatct gatcggtttg tattttgcct gtgcgctgga acgacatcaa aacgaacgcc
    44221 agccgatcat tttgctctcg gaccagaacg cgattgccac tattaatcag ctcgccattg
    44281 agcgcgatgt tttacattgt cgggtgatta ttgcccgtag cttaagcgaa cttgttgcca
    44341 ttcgcgaaga gattgagccg ttattgatca ttaacaacag ccattattta ctggatgacg
    44401 cggtaaataa ttacatcacc gtaaaaaata tcattacggc tgccggtatc gaacaaataa
    44461 aacattttct ggcgacggca tttattcgcc agcaaccgga gcgttttttc tctgcccccg
    44521 gaagttttca ttattcgaat gtacgcggtg aaagctggca acatattacc cggcaaattt
    44581 gtgcgcaatt agtcgcacaa caccatatta ccgccgatga agcgcaacgg atcatcgccc
    44641 gcgaaggcga aggtgaaaac ctgattgtta atcgcctcgc catcccacat tgctggagcg
    44701 aacaggagcg acgttttcgt ggatttttta ttaccctcgc ccaaccagtt gaggtgaata
    44761 acgaagtcat taaccatgtc ttgatcgcct gcgccgccgc cgatgcgcgt catgagctaa
    44821 aaatatttag ctatctggca agcgtattgt gtcagcatcc ggcagaagtt attgccgggc
    44881 taacaggata tgaggcattt atggagttac ttcacaaggg gtgaactgtg tcggatgcgc
    44941 cgcatccgac actgcattta tcatcaaaaa cgtttaacgc aggctattat agtcatcaac
    45001 catttcgttg tactcgcgta acgctcgtgg aaatgagtct tcgaccatcc agcttgtgtt
    45061 tgcatcctga agatgctctt tatcccagtc agagtacggg actttatctc tgacgcggcg
    45121 aacatatttt ttagtctcga gttgatactg gtctgccgaa ttaatgaacg agaaattcat
    45181 gccgccttta tccgcttctt tggcctgcgc caccagcgtt tccagttcag acactttctt
    45241 cattgccgca tcaacatcaa acttctcttg cgatatcagg ttattaattt gtttcgctga
    45301 aatcatgact gccagagaat agtagtggaa tgttttccct tctctttctt caatattttt
    45361 cagttccgca agctggcgct tatcattaat ttcctgaatc gccgcatgat aagattctgc
    45421 tacaggttca aaggcttcca gattttttaa gaatgtctgg tgcagcgttt tccctttcgc
    45481 aaacgcatcg tctttataat tttcctgggt gtaatattta tccatttcgt taatggtatt
    45541 acccaacgcc acggcagcat cgatataact caccgccacg ccatcaattg gttgcagcgc
    45601 aggcgttaac gccacagcac tttttacgcc tttttcacac tcagcgaggt tggattcact
    45661 aatgccgtag atgccataaa cagtactctc tttaccggta ggcccctgtt taaaatcttt
    45721 cagccagtca gcataacgcg ccagactgcg ctgtaccggg atctgtaact tgttgtagca
    45781 cttgatatag acatccattt tttcgtcgaa aaccgcttgt tcatcgacca gcggttgcgc
    45841 aggtgtttca ggtttagcat ctgcttttgc aacgggagct tcagtaggtt tcgcttcagg
    45901 agctggtgcg gcaggttgac tattggcagg caggggcgtt tctgtttcgg cttttttatc
    45961 atcacaaccc gtcagaccga gggccatgat ggcgactata atagcggaag ata
//
LOCUS       DAZFTN010000020        45076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFTN010000020 DAZFTN010000000
VERSION     DAZFTN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1081)
                     /gene="espY3"
                     /locus_tag="ACHKV2_001347"
     CDS             complement(<1..1081)
                     /gene="espY3"
                     /locus_tag="ACHKV2_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO6939267.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKI"
     gene            complement(1312..2286)
                     /gene="yajO"
                     /locus_tag="ACHKV2_001348"
     CDS             complement(1312..2286)
                     /gene="yajO"
                     /locus_tag="ACHKV2_001348"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO6939268.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2341..4203)
                     /gene="dxs"
                     /locus_tag="ACHKV2_001349"
     CDS             complement(2341..4203)
                     /gene="dxs"
                     /locus_tag="ACHKV2_001349"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO6939269.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(4228..5127)
                     /gene="ispA"
                     /locus_tag="ACHKV2_001350"
     CDS             complement(4228..5127)
                     /gene="ispA"
                     /locus_tag="ACHKV2_001350"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO6939270.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(5127..5369)
                     /gene="xseB"
                     /locus_tag="ACHKV2_001351"
     CDS             complement(5127..5369)
                     /gene="xseB"
                     /locus_tag="ACHKV2_001351"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO6939271.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5575..7023
                     /gene="thiI"
                     /locus_tag="ACHKV2_001352"
     CDS             5575..7023
                     /gene="thiI"
                     /locus_tag="ACHKV2_001352"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO6939272.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(7077..7667)
                     /gene="yajL"
                     /locus_tag="ACHKV2_001353"
     CDS             complement(7077..7667)
                     /gene="yajL"
                     /locus_tag="ACHKV2_001353"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO6939273.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7630..8541)
                     /gene="panE"
                     /locus_tag="ACHKV2_001354"
     CDS             complement(7630..8541)
                     /gene="panE"
                     /locus_tag="ACHKV2_001354"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO6939274.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8709..9200
                     /gene="yajQ"
                     /locus_tag="ACHKV2_001355"
     CDS             8709..9200
                     /gene="yajQ"
                     /locus_tag="ACHKV2_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO6939275.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9328..10692)
                     /gene="yajR"
                     /locus_tag="ACHKV2_001356"
     CDS             complement(9328..10692)
                     /gene="yajR"
                     /locus_tag="ACHKV2_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939276.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(10841..11731)
                     /gene="cyoE"
                     /locus_tag="ACHKV2_001357"
     CDS             complement(10841..11731)
                     /gene="cyoE"
                     /locus_tag="ACHKV2_001357"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO6939277.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11743..12072)
                     /gene="cyoD"
                     /locus_tag="ACHKV2_001358"
     CDS             complement(11743..12072)
                     /gene="cyoD"
                     /locus_tag="ACHKV2_001358"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO6939278.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12072..12686)
                     /gene="cyoC"
                     /locus_tag="ACHKV2_001359"
     CDS             complement(12072..12686)
                     /gene="cyoC"
                     /locus_tag="ACHKV2_001359"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO6939279.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12676..14667)
                     /gene="cyoB"
                     /locus_tag="ACHKV2_001360"
     CDS             complement(12676..14667)
                     /gene="cyoB"
                     /locus_tag="ACHKV2_001360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO6939280.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14689..15636)
                     /gene="cyoA"
                     /locus_tag="ACHKV2_001361"
     CDS             complement(14689..15636)
                     /gene="cyoA"
                     /locus_tag="ACHKV2_001361"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO6939281.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(16096..17571)
                     /gene="ampG"
                     /locus_tag="ACHKV2_001362"
     CDS             complement(16096..17571)
                     /gene="ampG"
                     /locus_tag="ACHKV2_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO6939282.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(17615..18193)
                     /gene="yajG"
                     /locus_tag="ACHKV2_001363"
     CDS             complement(17615..18193)
                     /gene="yajG"
                     /locus_tag="ACHKV2_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6939283.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            18498..18815
                     /gene="bolA"
                     /locus_tag="ACHKV2_001364"
     CDS             18498..18815
                     /gene="bolA"
                     /locus_tag="ACHKV2_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO6939284.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            19159..20457
                     /gene="tig"
                     /locus_tag="ACHKV2_001365"
     CDS             19159..20457
                     /gene="tig"
                     /locus_tag="ACHKV2_001365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6939285.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20702..21325
                     /gene="clpP"
                     /locus_tag="ACHKV2_001366"
     CDS             20702..21325
                     /gene="clpP"
                     /locus_tag="ACHKV2_001366"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO6939286.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            21451..22725
                     /gene="clpX"
                     /locus_tag="ACHKV2_001367"
     CDS             21451..22725
                     /gene="clpX"
                     /locus_tag="ACHKV2_001367"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO6939287.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            22913..25267
                     /gene="lon"
                     /locus_tag="ACHKV2_001368"
     CDS             22913..25267
                     /gene="lon"
                     /locus_tag="ACHKV2_001368"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO6939288.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            25458..25748
                     /gene="hupB"
                     /locus_tag="ACHKV2_001369"
     CDS             25458..25748
                     /gene="hupB"
                     /locus_tag="ACHKV2_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO6939289.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25940..27811
                     /gene="ppiD"
                     /locus_tag="ACHKV2_001370"
     CDS             25940..27811
                     /gene="ppiD"
                     /locus_tag="ACHKV2_001370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6939290.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27962..28333
                     /gene="ybaV"
                     /locus_tag="ACHKV2_001371"
     CDS             27962..28333
                     /gene="ybaV"
                     /locus_tag="ACHKV2_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO6939291.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            28439..28837
                     /gene="fadM"
                     /locus_tag="ACHKV2_001372"
     CDS             28439..28837
                     /gene="fadM"
                     /locus_tag="ACHKV2_001372"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO6939292.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(28889..29584)
                     /gene="queC"
                     /locus_tag="ACHKV2_001373"
     CDS             complement(28889..29584)
                     /gene="queC"
                     /locus_tag="ACHKV2_001373"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO6939293.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(29649..31349)
                     /gene="ybaE"
                     /locus_tag="ACHKV2_001374"
     CDS             complement(29649..31349)
                     /gene="ybaE"
                     /locus_tag="ACHKV2_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO6939294.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            31449..32267
                     /gene="cof"
                     /locus_tag="ACHKV2_001375"
     CDS             31449..32267
                     /gene="cof"
                     /locus_tag="ACHKV2_001375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO6939295.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            32419..32877
                     /gene="decR"
                     /locus_tag="ACHKV2_001376"
     CDS             32419..32877
                     /gene="decR"
                     /locus_tag="ACHKV2_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO6939296.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            32907..34679
                     /gene="mdlA"
                     /locus_tag="ACHKV2_001377"
     CDS             32907..34679
                     /gene="mdlA"
                     /locus_tag="ACHKV2_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6939297.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            34672..36453
                     /gene="mdlB"
                     /locus_tag="ACHKV2_001378"
     CDS             34672..36453
                     /gene="mdlB"
                     /locus_tag="ACHKV2_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6939298.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            36634..36972
                     /gene="glnK"
                     /locus_tag="ACHKV2_001379"
     CDS             36634..36972
                     /gene="glnK"
                     /locus_tag="ACHKV2_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO6939299.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            37002..38288
                     /gene="amtB"
                     /locus_tag="ACHKV2_001380"
     CDS             37002..38288
                     /gene="amtB"
                     /locus_tag="ACHKV2_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO6939300.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(38337..39197)
                     /gene="tesB"
                     /locus_tag="ACHKV2_001381"
     CDS             complement(38337..39197)
                     /gene="tesB"
                     /locus_tag="ACHKV2_001381"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO6939301.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            39415..39987
                     /gene="ybaY"
                     /locus_tag="ACHKV2_001382"
     CDS             39415..39987
                     /gene="ybaY"
                     /locus_tag="ACHKV2_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO6939302.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(40020..40331)
                     /locus_tag="ACHKV2_001383"
     CDS             complement(40020..40331)
                     /locus_tag="ACHKV2_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO6939303.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            40494..40590
                     /gene="ffs"
                     /locus_tag="ACHKV2_001384"
     ncRNA           40494..40590
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKV2_001384"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40710..41063
                     /gene="ybaA"
                     /locus_tag="ACHKV2_001385"
     CDS             40710..41063
                     /gene="ybaA"
                     /locus_tag="ACHKV2_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO6939304.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(41105..42655)
                     /gene="pdeB"
                     /locus_tag="ACHKV2_001386"
     CDS             complement(41105..42655)
                     /gene="pdeB"
                     /locus_tag="ACHKV2_001386"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO6939305.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42819..43289)
                     /gene="ylaC"
                     /locus_tag="ACHKV2_001387"
     CDS             complement(42819..43289)
                     /gene="ylaC"
                     /locus_tag="ACHKV2_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO6939306.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(43405..43956)
                     /gene="maa"
                     /locus_tag="ACHKV2_001388"
     CDS             complement(43405..43956)
                     /gene="maa"
                     /locus_tag="ACHKV2_001388"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO6939307.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(44128..44346)
                     /gene="hha"
                     /locus_tag="ACHKV2_001389"
     CDS             complement(44128..44346)
                     /gene="hha"
                     /locus_tag="ACHKV2_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO6939308.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(44372..44746)
                     /gene="tomB"
                     /locus_tag="ACHKV2_001390"
     CDS             complement(44372..44746)
                     /gene="tomB"
                     /locus_tag="ACHKV2_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO6939309.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        11346 a        11082 c        11856 g        10792 t
ORIGIN      
        1 taatttttgc taatgggcta atgtcttttt cttctgttga ttctgcttct gtttttaata
       61 tcggattaaa aaggtcggta cggtaaagtt tcgtgccttt catcctgacg atgtcaggaa
      121 gaatagtgtt tgaaagatta gcctcgctaa agtccacatt ttggcaatca ctttctgata
      181 aatctgccca attgaaagtg gttttcacac aggagcagtt agttagaatc atccctttcg
      241 tttcattccc atctgatttc tgataagtta tcctttgtat tatttgatct gcttctggat
      301 attgcatctc aggttctttt tctttaccat atatcatcgc attatcaaga attgcgccag
      361 tgcaattcat atttgtcagg tcgcatttct taaatctaca attgttaaaa ttcgcatttg
      421 tacaattcgc atttgtaaac ttgacgccat aaaaataagt atgttcgaag ttgacatttg
      481 agcagttaca gtttcccata tctataccga aatctatatc gggatcttta ttgggatctt
      541 taccggaaat attgctgtgt gctagatata gaccgctgaa atcgtaatat ccattgtcat
      601 ccttttctat tgggagtgaa taagtcttta ctacctcgtt aacaatagag atagttccac
      661 gtatcatcag attttctccg tctttataaa aaataatgtt atgtttgatg gtactgttgt
      721 atggctctgg caatgaggat gcaattttat tccagaaaca acaaagcgtg gtctgttttt
      781 gtttgggcga gaggcgatca aaatccagct ttaaatatga gtctaaatct gacgttgcat
      841 ctatattaaa gttgggatct ggagttaacg ctgaattata atttagaact gacaatgtat
      901 tgatgtattt atgaatcgat ttaaatgcct tttgtttatc actgtccaga aaaaaatcac
      961 gaattttgtt ccacagcctt tcaataagat tttgatttaa atcttgcttc attgagtttc
     1021 ttaaatctgc aaccggtaca tcagtaaaca ggatcggttg tttggtgatg gttttcatca
     1081 tgattgtaat cctcttacgt tgactaaata agacagtttt aggggatatt acctggagaa
     1141 actatcaaaa atcgctcaaa gggatgaaga cgatgaatag ctgcaaactc attcacgaag
     1201 aatttctctt gcagaggtgg attatggtta cagaaggtga tgggaggtac gttgtcccca
     1261 tcatcgcaga cgatggggaa ttcagcagat aaaatgggtg aaaagcgaaa tttacttaaa
     1321 tcctacgacc ggatgcggtt tatacggtgt ttccagttca gcaatctgtt ccggcttcaa
     1381 agtgatatcc accgcgttca gcagctcatc aagctgttct tcccgcgaag ttccgataat
     1441 cggtgcggca atgcccggtt tactcaacaa ccaggccagc gcaacttgtg ctcgtgttgc
     1501 accgagttct tcactgacgc ccgttaaccg ctctgcgatc tgcgcgtcat tttcatcgct
     1561 ttctttatag agatttttcc ccacctcatc agacaccagt cgtgcggtag tttctcccca
     1621 cggacgcgtc agtcgccccc gcgccagcgg actccacgga atgaccgcca cgccttcctg
     1681 ataacacagc ggcagcatct cgcgctcttc ttcgcgataa atcagattgt agtgatcctg
     1741 catactgaca aactgcgccc agccgtgctg tttttgtagt tccagtgcct gagcaaactg
     1801 cgaagcgtgc atggatgacg cgccgatata acgcgctttc ccggctttta ccacgtcgtt
     1861 gagggcttcc agcgtctctt cgatcggcgt gttgtaatcc cagcgatgaa tttgcaggat
     1921 atcgacataa tccatgccga gacgtcgcag gctgtcgtcg atagagcgca aaatttgtgc
     1981 acgggataat ccttccggta aatcaccaac gcgatggaac actttggtgg caacgaccac
     2041 gtcttcacga cgggagaaat cccgcagtgc gcgaccgacg atctcttcgc tgctgccatc
     2101 ggaatagcta ttggcggtat caaagaaatt tatgccgcct tccagtgcgc gtttaatgat
     2161 gggacggctg ctctcttccg gcagtgtcca tgcgtgatta ccacgatctg gctcgccaaa
     2221 ggtcatacag ccgaggcaaa gtcgggaaac gcgaaggtcg gtttttccta aggggttgta
     2281 ttgcatgctg ccactcctgc catgaataat ttcttaagca tagcaggagt ggagtaggga
     2341 ttatgccagc caggccttga ttttggcttc cataccggcg gcatcgaggc cgagttcggc
     2401 gcgcatttct tcctgagttc cttgcggaat aaagaagtcc ggcaggccaa tgttcagcac
     2461 gggtactggt ttacgatggg ccatcagcac ttcgttcact ccgctgcctg caccgcccat
     2521 aatggcgttt tcttctacgg taaccagtgc ttcatgactg gcggccattt ccagaattaa
     2581 cgtttcatca agcggtttca caaaacgcat atcgaccagc gtggcgttca gcgattcggc
     2641 gactttcgcc gcttctggca tcagcgtacc aaagttaagg atcgccagtt tctcgccacg
     2701 acgcttcaca atgcctttgc caattggtag tttttccagc ggcgtcagtt ccacgccgac
     2761 cgcgttgcca cgcgggtagc gcaccgctga cgggccatcg ttatagtgat agccggtata
     2821 gagcatctgg cgacattcgt tttcatcgct cggggtcata atgaccattt ccggtatgca
     2881 gcgcaggtaa gagagatcaa aagcgccctg gtgggtttga ccgtcagcac caacaatgcc
     2941 cgcgcggtcg atggcgaaca ggaccgggag tttttgaatt gccacgtcat gcagcacctg
     3001 atcataggcg cgttgcagga aggtggagta aattgccacg atcggtttgt agccaccaat
     3061 cgccagaccg gcggcaaagg tcaccgcgtg ttgctcggca atcgctacat caaaatagcg
     3121 atccgggaat ttacgtgaaa actcgaccat gccggaacct tcacgcatcg ccggagtaat
     3181 cgccatcagt ttgttatctt tcgctgccgt ttcgcacaac cagtcgccaa agatttttga
     3241 atagcttggt aaaccaccgc tacttttcgg caaacaaccg ctggagggat caaatttagg
     3301 cacggcgtgg aaggtgattg ggtctttttc tgccggttca taaccacgac cttttttggt
     3361 catgatatgc aggaactgcg ggcctttcag gtcgcgcatg ttcttcagcg tggtgataag
     3421 ccccagcaca tcgtgaccgt caaccgggcc gatgtagtta aagcccagct cttcaaacaa
     3481 cgtgccaggc accaccatgc ctttaatatg ttcttcggta cgtttgagca gctctttaat
     3541 tggcggaacg ccagagaaaa cttttttccc gccttcgcgc agtgaagagt aaagcttacc
     3601 ggaaagcagc tgcgccagat ggttgttaag cgcgccaaca ttttcggaaa tcgacatttc
     3661 attgtcgttg aggaccacca gcatatcagg acgaatatcg cccgcgtgat tcatcgcttc
     3721 aaacgccatg cctgcggtaa tcgcgccgtc gccaatcaca cagacggtgc ggcgattttt
     3781 gccttctttc tcggcagcaa ccgcaatacc aattccggca ctgatggagg ttgatgaatg
     3841 cccgacgctt aatacgtcat attcgctttc gccgcgccac gggaacgggt gtagaccgcc
     3901 tttctgacgg atggtgccga ttttgtcgcg gcgtccggtc aaaattttat gcggataagc
     3961 ctgatgcccc acatcccaaa tcaattggtc aaacggggtg ttatagacat agtgcagcgc
     4021 cacggtcagt tcgaccgtgc ccagcccgga ggcgaagtgc ccgctggaac ggctcacgct
     4081 gtcgagtaaa tagcggcgca gttcgtcgca gagtttcggt aaactctctt ttggcaacag
     4141 tcgtagctcc tgggtggagt cgaccagtgc cagggtcggg tatttggcaa tatcaaaact
     4201 catcaggggc ctattaacac ttattgttta tttattacgc tggatgatgt agtccgctag
     4261 cgcttccagt gccgaagtat cgatcgactg ttcagccagt tgtttcagcg actggcgggc
     4321 atcgtcgatc agatcctggg ctttcttccg ggcttgctca agacccagga gtgcagggta
     4381 ggtacttttg ccaagctgct ggtcggcacc ctgacgtttt cccaacgttg cagtatctcc
     4441 caccacatcc aggatgtcat cctgaacctg gaaggcaagg ccgatgctct ctgcgtactt
     4501 gtcgagtact ggtagagcac gacgtccttt atctccggcg cttaatgcac caaggcgaac
     4561 ggcggcgcga attaatgcgc ctgttttatg acgatgaata cgctcaagcg cgtccagagg
     4621 tacgtgtttg ccttccgcgt ctaaatccaa cgcctgacca ccacacattc cggcaatacc
     4681 gctggcgctc gccagttcag aaatcatcga aattctgtcg cgatccgaca cttctggcat
     4741 atcagcatcg cttaaaatcg agaacgccag cgtttgtaaa gcgtcgccag cgagaatcgc
     4801 gttcgcttcg ccaaacttca catggcaggt cggcaagccg cgacgcagat cgtcatcatc
     4861 catcgccggt aaatcatcat gaattaatga gtaagcgtgg atacactcta cggcggcagc
     4921 gggtgcgtcc agcgtgtttg tgctaacgcc gaacatatga ccggtggcat aaaccaggaa
     4981 aggtcgcagg cgcttaccac ctaataatgc gccatactgc atggtttcga ccacgggagt
     5041 gttctgaaag ggcagtgggg cgataaaacg gctcagcgcc tggttggcct gcttaacgca
     5101 ggcttcgagt tgctgcggaa agtccattac tcattgtccg gtgtaaaagg ggttagagag
     5161 gcgtcttcgt tgtcagacag cagaatttgt acgcgctgtt cggcttgttg taatttggcc
     5221 tgcccctgac gtgccagctg cacgccgcgt tcgaactcgt tcagcgcctc ttccagcggc
     5281 aggtcgccac tttccagacg ggttacaatc tgttccagct cgctcagcgc cttttcaaag
     5341 ctggcgggcg cctcattttt cttcggcata atgaatgtct gactctcaat atttttcgcc
     5401 ccgtcatggt aacggactca gggcaaatag caaataacgc gcaatggtaa ggtgatgtgc
     5461 gcagcaaagc gatgttagtg gtatacttcc gcgcctggat gcagccgcag gtgtgggctg
     5521 ctgtattttt ccctatacaa gtcgcttaag gcttgccaac gaaccattgc cgccatgaag
     5581 tttatcatta aattgttccc ggaaatcacc atcaaaagcc aatctgtgcg cttgcgcttt
     5641 ataaaaatcc ttaccgggaa cattcgtaac gttttaaagc actatgatga gacgctcgct
     5701 gtcgtccgcc actgggataa catcgaagtt cgcgcgaaag atgaaaacca gcgtctgact
     5761 attcgcgacg ccctgacccg tattcctggt atccaccata ttctcgaagt cgaagacgtg
     5821 ccgtttaccg acatgcacga tattttcgag aaagcgttgg ttcagtatcg cgatcagctg
     5881 gatggcaaaa ccttctgcgt acgcgtgaag cgccgtggca aacatgattt tagctcgatt
     5941 gatgtggaac gttacgtcgg cggcggttta aatcagcata ttgaatccgc gcgcgtgaag
     6001 ctgaccaatc cggatgtgac tgtccatctg gaagtggaag acgatcgtct cctgctgatt
     6061 aaaggccgct acgaaggtat tggcggtttc ccgatcggca cccaggaaga tgtgctgtcg
     6121 ctcatttccg gtggtttcga ctccggtgtt tccagttata tgttgatgcg tcgcggctgc
     6181 cgcgtgcatt actgcttctt taacctcggc ggcgcagcgc atgaaattgg cgtgcgtcag
     6241 gtggcacatt atctgtggaa ccgctttggc agctcccacc gcgtgcgttt tgtcgctatt
     6301 aatttcgagc cggtagtcgg ggaaattctc gagaaaatcg acgacggtca gatgggcgtt
     6361 atcctcaaac gtatgatggt gcgtgccgcg tctaaagtgg ctgaacgtta cggcgtacag
     6421 gcgctggtca ccggcgaagc gctcggccag gtgtccagtc agacgctgac caacctgcgc
     6481 ctgattgata acgtctctga tacgctgatc ctgcgtccgc tgatctctta cgacaaagag
     6541 cacatcatca acctggctcg ccagattggc accgaagact ttgcccgtac tatgccggaa
     6601 tactgcggtg tgatctccaa aagcccgacg gtgaaagcgg ttaagtcgaa gattgaagcg
     6661 gaagaagaga aattcgactt cagcattctc gataaagtgg ttgaagaagc gaataacgtt
     6721 gatatccgcg aaatcgccca gcagaccggg caggaagtgg tggaagtgga aaccgtcaat
     6781 gacttcggcc cgaacgacgt gattctcgat atccgttctg tcgatgaaca ggaagataag
     6841 ccactgaaag tcgaagggat tgatgtggtt tcactgccgt tctacaagtt gagcaccaaa
     6901 tttggcgatc tcgaccagaa cagaacctgg ctactgtggt gtgagcgcgg ggtaatgagc
     6961 cgcctgcagg cgctctatct acgcgaacag ggctttaaga atgtgaaggt gtatcgtccg
     7021 taatttgtgg tttttacgtc gcatctggtc agatgcgacg tttgccgcat ccgacactac
     7081 tcgtaataat tataaatccc tgccgccatc accagttgtg atgccacttc atgggctttc
     7141 tcgcgcccaa ccagcaggtc gataattttc agaccaaagt cgatagctgt acccggcccc
     7201 tggctggtca gcaattttac ccgtgcatcc cagacgacgc gcttgtcctg ccattgttca
     7261 gcgggaattt tgtctttcag cgtcgggaag ccggtcatat tgccaatcgg aaagatatcg
     7321 tgcggcacca gcacggtggc tggcgcggcg caaatagccg cgacgatacg cccggaacgg
     7381 tggaactgtt taacggtttc aaccagcagg gtactatcgc gaaaacactc cgcgccttta
     7441 atgccaccag gcagcacgat cacgtcatat tcgccatcag ccacttcgac cagcggcgca
     7501 tccgccagca gcttcacgcc gcgcgagcag gtaatcgcca ggttgccatc gctggctacg
     7561 ctggcagtgg tgactttgat accgccgcga atcaagaggt cgatcgtggt gacggcttca
     7621 gtctcttcac taccaggggc gaggcaaacc agtgccgatg cgctcatatt cactctcctt
     7681 tctttttacc atttcaaaca ggcgggtgtt ttccggtacg gcaatcccat gcgcgcgggc
     7741 gcggcgtaag agaaaaccat tgatatagtc gatttcggtg tggcgcagcg tgcggatatc
     7801 ctgcaacatc gacgagatat tttccgctgt ggcatcaatc acctgcatca cgtaatcacg
     7861 taaatcttcc gctgaagtat gatgcccttc gcgttcgatc actgccgcga cttcttcgca
     7921 tatttgcata atttcttgcg gatgatgacg taattcaccg ttcgggcaat tccagatggc
     7981 agtcagtgga ttaatcacac agttgactgc cagcttgcgc cacagctcgg cgcgaatatt
     8041 gttatgccag gcaacgtcag gcaacacggt ttgcaatata tccgccagat aactgtaatc
     8101 gccgtcctgt tgccgtgctg gaccaatatg cgtgataccg tttgccacat gaataatgac
     8161 attgccgtcg cggcaggcgg catgggtggt ggtgcccatc agtaatggct gctgaatgtt
     8221 ttgcaattct tcgatggtgc ccatgccgtt gtgaattaac agtattggcg tagttacagg
     8281 cagtgtggac gcgaggcttt tgacggcatc ggaaacctgc catgctttca gcgtcaccag
     8341 gagcagatcg ctggtggcaa gaaaatcggg atcgttggcg gtcagcgatt cgttaaatat
     8401 cgaaccatca gtctcaacca gattcacact acaataaggt tgcggtacgc gcagccagcc
     8461 ctgaacttcg tgaccctgtt tacaaagtgc tgtaagccat aattgcccta aggcaccgca
     8521 tcccaatacg gtaattttca ttgttcctcc tcacccgcaa ccactccggg tgttcaataa
     8581 ggctatccct taattgtgca tgctgttgcg actatgcaca attaagggat acgtcctggt
     8641 gcaggactgt tggttattta actttgcagg tattatgctt cgcatcaaaa atgaagggag
     8701 aggaaaagat gccatctttc gatattgtct ctgaagttga tcttcaggaa gcacgtaacg
     8761 cggtcgataa cgcgagccgc gaagtggagt cccgttttga cttccgtaac gttgaagcct
     8821 catttgagct gaacgacgcc agcaaaacca tcaaagtgtt gagcgagtcc gacttccagg
     8881 tcaatcagtt gctggatatt ctgcgtgcca agctgctgaa gcgcggcatt gaaggcagtt
     8941 cactggatgt accggaaaat atcgttcata gcggtaaaac ctggtttgtg gaagcgaaac
     9001 tgaaacaggg cattgagagc gccactcaga aaaaaatcgt caagatgatc aaagacagca
     9061 aactgaaagt gcaggcgcaa attcagggcg atgagatccg tgtgacgggc aaatctcgtg
     9121 atgatttaca ggctgtcatg gcgatggtgc gcggtggcga tctcggtcag ccgttccagt
     9181 tcaaaaactt ccgcgattaa tcgcgacaac ctgcgctttg ttcatgtcag atgcggcatg
     9241 aacgcctggt tcggcctaca aagttttgta aatcaggaac ttagtaggcc tgataagcgt
     9301 agcgcagcag gcaatttcgc ctcgctttta tgcctggcga attgcctgtt caacatcaaa
     9361 gcgattcgtc actttgctgt cgattttcac ataagctgaa tgttcttctt ctgcaatcaa
     9421 cacttctttg atgccttcag tttccagcaa acgcactttt aacgcctcgt ttgcggcaat
     9481 gttcgccggg atttcaatgc gcaaactgct gacatacggc ggttctttca tggtactggc
     9541 gactgccagc cacactgcgg ccagcattgc gccagcgaga aataccccct gaccgtcaaa
     9601 catgccgtca atccagccgc ccagcgaacc gccaatcgcc acgccaagaa actggctggt
     9661 ggagtaaaca cccatcgccg tacctttgta acctgctggc gactctttac tgataagtga
     9721 gggcaggagg gcttccatca aattaaacgc cacaaagaaa agctgcacgc cgaccaccag
     9781 ttgccagaac tgcgtttgcg cgttcctcaa cacaatttcc gcaaccacga tcaacccgac
     9841 gcagaagaca aagacttgct tcattttgcg cttaacttca gcgtagataa tgaaaggcac
     9901 gaccgagcca aaggcgatta gcattgtcgc cagatagacc ttccagtgtt cagccgccgg
     9961 gaaccccgca tcagccagtt gtccgggcag ggcaacaaac gtcgacatca gcaaaatatg
    10021 cagacacata atgccaaagt tgagtttcag cagccgcggt tccgccagca ccttactgaa
    10081 actgcctttc accattccgg actcacgatt aagtacgtga gtgctactgt tgggcacaac
    10141 ccaaatggtc aacgcaatgc cggtcgttgc cagaatagcg atcatccaga acagcgcgtg
    10201 cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa aggtaatgcc
    10261 aaagctcacg ccgataaacg ccattgcttt ggtgcggttt tgttcgcgcg tgagatcgga
    10321 aagcagcgcc ataacggcag cggcaatcgc accggagcct tgtagcgccc gacccagaat
    10381 aattccccag atggagtcag agagcgcagc gataacgcta ccggcggcaa acactgccag
    10441 cccaccgaca attaatggtt tgcgaccaat gcggtcggaa agcaggccaa acggaatctg
    10501 aaaaacggcc tgagtcagac cataaatacc aatggcaata ccgattaatg cttcgctggc
    10561 accttgcaga gccatgccgt acgtggtcag aaccggcaga accatgaaca tgcccagcat
    10621 gcgcaacgag aatacggtcc ctaaacccca ggtcgcgcgc cgctcacctg gcgtcatttt
    10681 ataatcgttc attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg gcaaacgaat
    10741 ggggtaaaca tgcggatttt ttagtaaata tttcgagagg gatatgtttc taatgctaag
    10801 aaaaaaggtg ccgtagcacc tttttaatag agaggttgtg ttaccacaca gcagccagca
    10861 gcgtatgcga gtccggcacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg
    10921 cgatgataga gaagccgaac agcttgcgcg cccagattct gtcatcagca actttataac
    10981 cgcgcagagc catacctaac caccagacgc taaccgccgc ggcgaccacc agatatttat
    11041 atccagcgta accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca
    11101 gcgtgatgtg attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg
    11161 ccgcctggta atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca
    11221 ggctgaagat agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt actgcacagt
    11281 agccaatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagacggagt
    11341 ggcgtttcat gtacaggcta taaacgccga cataaaccac aaagcccatc acccccagcc
    11401 agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg
    11461 tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg
    11521 tcctttccat ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg
    11581 ccacaaccag tgacacccca accagcgtgt agataaacag gggataatca atgctgccct
    11641 ttgaggccag caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt
    11701 tcgttacttg caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt
    11761 gtagttgagg ttccacataa tccagatgga gcctacaact aggatagcga tgattagcac
    11821 ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa
    11881 gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac
    11941 ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga
    12001 caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt
    12061 agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc
    12121 acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt agtgctggtc
    12181 aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc ggaagtgacg
    12241 tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct gcgatccgga
    12301 cccatgccgt taacaatcag gtgatggaat tcgtagattt ccatcccgat aaatccggcg
    12361 ccaaacaacc aggtcaacgc cagccaggag ataacctggc ttttgttgtt tttgtacatg
    12421 gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga
    12481 acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc gttcaccaga
    12541 acggcatagg tagcaaacaa gatagagaac agaatgcagt cgctcatcag gtagatccaa
    12601 aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtggacagtc
    12661 gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg
    12721 ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa
    12781 gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat
    12841 atgccagatc atggcgaaac cgaagatggt ggagaaagcc gcaatgacga tacctgcacc
    12901 gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg cttcgccttt
    12961 ctctttcatt tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg caaagttata
    13021 gaacggaggc ggggaagagg ttgcccactc cagcgtacgg ccaccccacg ggtcgccagt
    13081 caggtcacgg ttctggtcgc ggtcacgaat agaaacgtac atctgaataa cgaggcagag
    13141 aatacctagc gcaatcagta ctgcaccgct ggctgcaatc atcagcatgg tgtggaactg
    13201 cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg cgtacagtgg
    13261 cataaaggca acgaagaagc cgatgatcca gaaccaaaac gcgcgtttac cccaggtttc
    13321 gttcagtttg aaaccgaacg ctttaggcca ccagtaggtc atccctgcga agcagccgaa
    13381 gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc tgttatgtag
    13441 aacgaagtcc gcgcccggta ctgccagcag cacgccagtc ataccgccca ccgagaaggt
    13501 gacgataaaa ccgatagtcc acagcatcgc agaatggaac acgatgcggc cctgatacat
    13561 ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca ttgtggtgat
    13621 accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt gcagccaaac
    13681 gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat aaccaaacag
    13741 acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag gcaggatcag
    13801 gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca tcatgttgcc
    13861 acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca acaacgcgac
    13921 ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca gtgatgccca
    13981 ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca gaatggtaac
    14041 gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac tccatatcca
    14101 gtaatcgaca ccgactcccg gactgtactc tattcccgat agcggtggat aggccagcca
    14161 gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca caccaacaac
    14221 ggtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg cgccgatctg
    14281 cagcggaacc accaagttca tcagaccgat aacgaaaggc atcgccacga agaagatcat
    14341 aatcacgccg tgcgcggtga agatctgatc gtagtggtga ggtggcagga agcccgcttc
    14401 gcccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa aaccacgcag
    14461 caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt cgacggaggt
    14521 cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc caacgagcgc
    14581 cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat ggaacgggac
    14641 tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt aatgggcgga
    14701 ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcgctgtgct caccttctgg
    14761 ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaattacat cggcaaacaa
    14821 gtttggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg cggccagttt
    14881 ttcgaacgct gccatgtcag acatggagtt cggcgactgt ttcgcttttg cgacccactg
    14941 gtcgaatgcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg agaagcccgg
    15001 gccgctgtag ctggcggaga taccgtcata agtgccgggt tcgttggcga tcagatgcag
    15061 gcgagtctgc ataccggcca tggcataaat ctggctaccc agacgcggaa tgaagaagga
    15121 gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg ggaaagcgat
    15181 ttcattcacg gtagcaaagc cctgttccgg gtagatgaag aaccatttcc agtccatgga
    15241 aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag gctcaagagc
    15301 gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg attaagatag gtaccgtcca
    15361 gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag catctttatt
    15421 gctcgcacgg tacttccagg cgaaaccaac agccatcaag attgcgggaa taacgacaat
    15481 caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa tctgtccttt
    15541 gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa ataatgacaa
    15601 ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat tccacgggat
    15661 ctggtggcgt ttaaaatgtg tgggcatttt acgggaaggt tacattactg taaacatgat
    15721 taaattcctg ttacttggat ttggctgtca ggtcacatat gttgcaaaac atatcaggtt
    15781 gattttaaga agctgttgca aaagggcgtt ataacaaaag gggagtgcag aagaagtctg
    15841 gacgggcaat tggtataatc aatgcgaaat aaaacaatta tttaacaaat aattatcatt
    15901 ggagtgacaa cttggtggtg agaaaataaa gactcattag ctgaatcgtg taagaaaaat
    15961 agaatttata aatggcgcca ataatttcca aacgctaagc cgcacaaaag aacaaatatt
    16021 aataagcgat gctgatgttt atttagccgt aataattacg gcgagtgatt actacagcta
    16081 aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat ccagcaaacc
    16141 accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag caggcaggaa
    16201 agagaagtgc gtcaaatcca gcgcgtccat cgtcaatagc agtaaccaca cggccaacag
    16261 gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat atgcggtacg
    16321 ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa ccagcagcaa
    16381 aataagccct ggtacggcag cggcgacgga gaatagatag aatgtcgacc agccgtgtgc
    16441 ttcaacaaac caacccgcca cggggccgac ataaactcgc cctacagcag aaagcgctga
    16501 gagcagggca aattgagtag cggaaaatga cttattacat agcgtcatta acagcgcgac
    16561 aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg cgcccatgct
    16621 gtagagatgc ttatcagtaa tcgacagcag ccagtaacca gcgttagacg caccttgtaa
    16681 aatgccgaaa atcagcagtg cccggaacag tgacaggcgc tgcatcaaaa tcccaccgta
    16741 caatgcgcca acaatggtcg ctagtaagcc aagcgttttg tttaccacgc ctacttcacc
    16801 cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg cgaatgcgtc
    16861 gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc gaccaaagaa
    16921 atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttg ggcacaggaa tggtgtcggt
    16981 tggttctggt gcaagcaacg tcgcgataat acaggggatc aacagcgccg ccatcaacca
    17041 gtacatgccc tgccagccca gccatttatc tgccagccac agggctaggc cgccggaaac
    17101 cagcatccct aaacggtaac ccagaacgct gattgccgcg cccgcaccac gttcttctgc
    17161 cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggcag agcaaaaagc
    17221 gatcaccacc gccagcgccg ccatccagcg gagttgggtg cctggttcga gaaaacccat
    17281 cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc gccgccgccc
    17341 aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa aaacgtaagc
    17401 ctggcctacc agagagaaga aaccaatggt tttgagatcg atattctcga ccgtcatcca
    17461 ggcctgtaag gtgccggatg tcagggcgag cggtagcccg gaagcaaaac ccaggatcag
    17521 caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca tgggcgtcta
    17581 caggcccggc ttgcaccggg ccagagggca gaaattaacg cgcgttctgc ttgatgaatt
    17641 cgtgaatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca ctgttaaccg
    17701 catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag ctggcacgat
    17761 agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata tccgctttgg
    17821 tagcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagctgg ctaacgatga
    17881 tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc atctgttttt
    17941 ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta acgatttgat
    18001 tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca ttaatgctta
    18061 cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg gaaacttcaa
    18121 tagttgttgg cggtcttgcg catcctgcaa gcataaacag agcaactaac gggaagagga
    18181 tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa aaattcccgc
    18241 catcataaca ttgccaacgg cgaggggaag taggtaaggc atgtaaattc atcatgttga
    18301 cgaaataatc gcccctggta aaagaaacgc tgatgcgagg cctgtgtttc aatctttaaa
    18361 tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat ttgttgttaa
    18421 gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca tctcagcgtt
    18481 gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta agggcggcgt
    18541 tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc ccagccggct
    18601 ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa cgttttctga
    18661 atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc gttcatgcgc
    18721 tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc gtctttgcct
    18781 ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac tgtcggcgct
    18841 tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt tgttttgtct
    18901 ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg tgaaaaaata
    18961 cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat aatgccgcgt
    19021 cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat tataagaaca
    19081 tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc gagcactgtg
    19141 attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct tggccgccgt
    19201 gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga gctggtcaac
    19261 gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat gaatatcgtt
    19321 gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat gagccgtaac
    19381 ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac ttatgttccg
    19441 ggcgaataca agctgggtga agacttcacc tactctgtag agtttgaagt ttatccggaa
    19501 gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga agtgaccgac
    19561 gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg gaaagaaaaa
    19621 gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc tgtagacggc
    19681 gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca gggtcgtatg
    19741 atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt caccatcgac
    19801 gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc gaaattcgct
    19861 atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga attcatcaaa
    19921 cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg taaaaacatg
    19981 gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat cgaaggtctg
    20041 gtaaaagcta acgacatcga tgtaccggct gcgcttattg acagcgaaat cgacgttctg
    20101 cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga actgccgcgc
    20161 gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct gggcgaagtt
    20221 atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat cgaagagatg
    20281 gcttcagcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa caaagaactg
    20341 atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt actggcgaaa
    20401 gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca ggcgtaattt
    20461 acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg tgggcttttt
    20521 ttgtcatgaa ttttgcatgg aaccgtgcga aaagcctctt tcggtgttag cgtaacaaca
    20581 aaagattgtt atgcttgaaa tatggtgatg ccgtacccat aacacaggga ctagctgata
    20641 atccgtccat aaggttacaa tcggtacagc aggttttttc aattttatcc aggagacgga
    20701 aatgtcatac agcggcgaac gagataactt tgcaccccat atggcgctgg tgccgatggt
    20761 cattgaacag acctcacgtg gtgagcgctc ttttgatatc tattctcgtc tacttaagga
    20821 acgcgtcatt tttctgactg gccaggttga agaccatatg gctaacctga ttgtggctca
    20881 gatgctgttc ctggaagcag aaaacccaga aaaagatatc tatctgtaca ttaactcccc
    20941 aggcggggtg atcactgccg ggatgtctat ctatgacacc atgcagttta tcaagcctga
    21001 tgtcagcacc atctgtatgg gccaggcggc ctcgatgggc gctttcttgc tgaccgcagg
    21061 ggcaaaaggt aaacgttttt gcctgccgaa ttcgcgcgtg atgattcacc aaccgttggg
    21121 cggctaccag ggccaggcga ccgatatcga aattcatgcc cgtgaaattc tgaaagttaa
    21181 agggcgtatg aatgaactta tggcgcttca tacgggtcaa tcattagaac agattgaacg
    21241 tgataccgag cgcgatcgct tcctttccgc ccctgaagcg gtggaatacg gtctggtcga
    21301 ttcgattctg acccatcgta attgatgcca gaggcgcaac tgtgccgcta tacttatcca
    21361 gggcggcaca acgctgtaag cggcttgcgc ctgagaatgg catttgcgtc gtcgtgtgcg
    21421 gcacaaagaa caaagaagag gttttgaccc atgacagata aacgcaaaga tggctcaggc
    21481 aaattgctgt attgctcttt ttgcggcaaa agccagcatg aagtgcgcaa gctgattgcc
    21541 ggtccatccg tgtatatctg cgacgaatgt gttgatttat gtaacgacat cattcgcgaa
    21601 gagattaaag aagttgcacc gcatcgtgaa cgcagtgcgc taccgacgcc gcatgaaatt
    21661 cgtaaccacc tggacgatta cgttatcggc caggaacagg cgaaaaaagt gctggcggtc
    21721 gcggtataca accactacaa acgtctgcgc aacggcgata ccagcaatgg cgtcgagttg
    21781 ggcaaaagta acattctgct gatcggtccg accggttccg gtaaaacgct gctggctgaa
    21841 acgctggcgc gcctgctgga cgtcccgttc accatggccg acgcaaccac gctgaccgaa
    21901 gccggttatg tgggcgaaga cgttgaaaac atcattcaga agctgttgca aaagtgcgat
    21961 tacgacgtac agaaagcgca gcgcgggatt gtctacatcg atgaaatcga caagatttct
    22021 cgtaagtcag acaacccgtc tattacccgt gacgtttccg gtgaaggcgt acagcaggca
    22081 ctgttgaaac tgatcgaagg tacggtagct gctgttccac cgcaaggtgg acgtaaacat
    22141 ccgcagcagg aattcttgca ggttgatacc tctaagatcc tgtttatttg tggcggtgcg
    22201 tttgccggtc tggataaagt gatttcccat cgtgtagaaa ccggctccgg cattggtttt
    22261 ggcgcgacgg taaaagcgaa gtccgacaaa gcaagcgaag gcgagctgct ggcgcaggtt
    22321 gaaccggaag atctgatcaa gtttggtctt atccctgagt ttattggtcg tctgccggtt
    22381 gtcgcaacgt tgaatgaact gagcgaagaa gctctgattc agatcctcaa agagccgaaa
    22441 aacgccctga ccaagcagta tcaggcgctg tttaatctgg aaggcgtgga tctggaattc
    22501 cgtgacgagg cgctggatgc tatcgctaag aaagcgatgg cgcgtaaaac cggtgcccgt
    22561 ggcctgcgtt ccatcgtaga agccgcactg ctcgatacca tgtacgatct gccgtccatg
    22621 gaagatgtcg aaaaagtggt tatcgacgag tcggtaattg atggtcaaag caaaccgttg
    22681 ctgatttatg gcaagccgga agcgcaacag gcatctggtg aataattaac cattcccata
    22741 caattagtta accaaaaagg ggggatttta tctccccttt aatttttcct ctattctcgg
    22801 cgttgaatgt gggggaaaca tccccatata ctgacgtaca tgttaataga tggcgtgaag
    22861 cacagtcgtg tcatctgatt acctggcgga aattaaacta agagagagct ctatgaatcc
    22921 tgagcgttct gaacgcattg aaatccccgt attgccgctg cgcgatgtgg tggtttatcc
    22981 gcacatggtc atccccttat ttgtcgggcg ggaaaaatct atccgttgtc tggaagcggc
    23041 gatggaccat gataaaaaaa ttatgctggt cgcgcagaaa gaagcttcaa cggatgagcc
    23101 gggtgtaaac gatcttttca ccgtcgggac cgtggcctct atattgcaga tgctgaaact
    23161 gcctgacggc accgtcaaag tgctggtcga ggggttacag cgcgcgcgta tttctgcgct
    23221 ctctgacaat ggcgaacact tttctgcgaa ggcggagtat ctggagtcgc cgaccattga
    23281 tgagcgagaa caggaagtgc tggtgcgtac tgcaatcagc cagttcgaag gctacatcaa
    23341 gctgaacaaa aaaatcccac cagaagtgct gacgtcgttg aatagcatcg acgatccggc
    23401 gcgtctggcg gataccattg ctgcacatat gccgctgaaa ctggctgaca aacagtccgt
    23461 tctggagatg tccgacgtta acgaacgtct ggaatatctg atggcaatga tggaatcgga
    23521 aatcgatctg ctgcaggttg agaaacgcat tcgcaaccgc gttaaaaagc agatggagaa
    23581 atcccagcgt gagtactatc tgaacgagca aatgaaagct attcagaaag aactcggtga
    23641 gatggacgac gcgccggacg aaaacgaagc cctgaagcgc aaaatcgacg cggcgaagat
    23701 gccgaaagag gcaaaagaga aagcggaagc agagttgcag aagctgaaaa tgatgtctcc
    23761 gatgtcggca gaagcgaccg tagtgcgtgg ttatatcgac tggatggtac aggtaccgtg
    23821 gaatgcgcgc agcaaggtca aaaaagacct gcgtcaggcg caggaaatcc ttaataccga
    23881 ccattatggt ctggagcgcg tgaaagatcg catccttgag tatcttgcgg ttcaaagccg
    23941 tgtcaacaaa atcaagggac cgatcctttg cctggtaggg ccgccggggg taggtaaaac
    24001 ctccctgggt cagtccattg ccaaagccac cgggcgtaaa tatgtccgta tggcgctggg
    24061 cggcgtgcgt gatgaagcgg aaatccgtgg tcaccgccgt acttacatcg gttctatgcc
    24121 gggtaaattg atccagaaaa tggcgaaagt gggcgttaaa aacccgctgt tcctgctcga
    24181 tgagatcgac aaaatgtctt ctgacatgcg tggcgatccg gcttccgcac tgcttgaagt
    24241 gctggatcca gagcagaacg tggccttcag cgatcactac ctggaagtgg attacgatct
    24301 cagcgacgtg atgtttgtcg cgacgtcgaa ctccatgaac attccggcac cactgctgga
    24361 tcgtatggaa gtgattcgcc tctccggtta taccgaagat gaaaaactga acatcgccaa
    24421 acgtcacctg ctgccgaagc agattgaacg taatgcactg aaaaaaggtg agctgaccgt
    24481 cgacgatagc gccattatcg gcattattcg ttactacacc cgtgaggcgg gcgtgcgtgg
    24541 tctggagcgt gaaatctcca agctgtgccg taaagcggtt aagcagttac tgctcgataa
    24601 gtcattaaaa catatcgaaa ttaacggcga caacctgcat gactaccttg gtgttcagcg
    24661 tttcgactat ggtcgcgctg ataacgaaaa ccgtgtcggt caggtaaccg gtctggcgtg
    24721 gacggaagtg ggcggtgact tgctgaccat tgaaaccgca tgtgttccgg gtaaaggcaa
    24781 actgacctat accggttcgc tcggcgaagt gatgcaggag tctattcagg ctgcgttaac
    24841 ggtggttcgc gcgcgtgcgg aaaaactggg gatcaaccct gatttttacg aaaaacgtga
    24901 catccacgtc cacgtaccgg aaggtgcgac gccgaaagat ggtccgagtg ccggtattgc
    24961 tatgtgcacc gcgctggttt cttgcctgac cggtaacccg gttcgtgccg atgtggcaat
    25021 gaccggtgag atcactctgc gtggtcaggt actgccgatc ggtggtttga aagaaaaact
    25081 actggcagcg catcgcggcg ggattaaaac agtgttaatt ccgttcgaaa ataaacgcga
    25141 tctggaagag attcctgaca acgtaattgc cgatctggac attcatcctg tgaagcgcat
    25201 tgaggaagtt ctgactctgg cgctgcaaaa tgaaccgtct ggcatgcagg ttgtgactgc
    25261 aaaatagtga cctcgcgcaa aatgcactaa taaaaacagg gctggcaggc taattcgggc
    25321 ttgccagcct ttttttgtct cgctaagtta gatggcggat cgggcttgcc cttattaagg
    25381 ggtgttgtaa ggggatggct ggcctgatat aactgctgcg cgttcgtacc ttgaaggatt
    25441 caagtgcgat ataaattata aagaggaaga gaagagtgaa taaatctcaa ttgatcgaca
    25501 agattgctgc aggggctgat atctctaaag ctgcggctgg ccgtgcgtta gatgctatta
    25561 ttgcttccgt aactgaatct ctgaaagaag gggatgatgt agcactggta ggttttggta
    25621 cttttgccgt taaagagcgt gctgcccgta ctggccgcaa cccgcagacc ggtaaagaga
    25681 tcaccatcgc tgccgctaaa gtaccgagct tccgtgcagg taaagcactg aaagacgcgg
    25741 taaactaagc gttgtcccca gtggggatgt gacgaagttc aagggcgcat ctactgatgt
    25801 gcctttttta tttgtattcg gtgactttct gcgtcttgtg ggctgacaat tgcccccgtt
    25861 tcttgtcaca ataggccttt gcgcgcatcg atacgttgcg tgaggtacac agtcatctac
    25921 agcggagtgt tgttacacca tgatggacag cttacgcacg gctgcaaaca gtctcgtgct
    25981 caagattatt ttcggtatca ttatcgtgtc gttcatattg accggcgtga gtggttacct
    26041 gattggcgga ggcaataact acgccgcaaa agtgaatgac caggaaatca gccgtgggca
    26101 attcgagaat gccttcaaca gcgagcgtaa tcgcatgcag caacagctgg gcgatcaata
    26161 ctccgagctg gcagcgaacg aaggctatat gaaaaccctg cgtcaacagg tgctgaatcg
    26221 tctgatcgac gaggcgctgc tggatcagta cgcacgtgag ctgaaactgg gtatcagcga
    26281 tgagcaggtt aaacaggcga ttttcgcgac cccagccttc caggttgatg gcaaatttga
    26341 taacagccgc tataacggta tcctcaatca gatggggatg accgccgatc agtacgccca
    26401 ggcgctgcgt aaccagctca ctacccaaca gctgattaac ggcgttgctg gtaccgattt
    26461 tatgctgaaa ggtgaaaccg acgagctggc ggcactggtc gctcaacaac gcgtggtgcg
    26521 tgaagcgact atcgatgtta acgcgctggc ggcgaagcag cctgtgaccg aacaggaaat
    26581 tgccagctac tacgaacaaa acaaaaacaa tttcatgacg ccggaacaat tccgcgtgag
    26641 ttacatcaag ctggatgccg caacgatgca gcaaccggtt agcgatgcgg atctccagag
    26701 ctactacgat cagcatcagg atcaattcac ccagccgcag cgtacccgtt acagcatcat
    26761 ccagaccaaa actgaagatg aagcgaaagc ggtacttgat gagctgaata aaggcggtga
    26821 ttttgctgca ttagccaaag aaaaatctgc cgatattatc tctgctcgta acggcggcga
    26881 tatgggttgg ttagaagatg ccactatccc ggatgaactg aaaaatgctg gtctgaaaga
    26941 aaaaggccaa ctgtctggtg tcatcaaatc ttcggtcggt ttcctgattg tacgtctgga
    27001 cgacattcag ccagcgaaag tgaaatcgtt agacgaagta cgtgacgatg tcgcggcgaa
    27061 agtgaaacac gaaaaagccc tcgatgcgta ctacgcgctg cagcagaaag tgagcgatgc
    27121 ggcaagcaac gacaccgagt ctctggccgg tgcagagcaa gctgccggcg ttaaagccac
    27181 tcagacgggt tggttcagca aagagaacct gccggaagag ttgaacttca agccggttgc
    27241 tgacgctatc tttaacggcg gtctggtagg tgaaaacggc gcgccgggca tcaactctga
    27301 catcatcacc gtagacggcg accgcgcatt cgtgctgcgc atcagcgagc acaaaccgga
    27361 agcggtgaaa ccgttggcag atgttcagga acaagttaag gcactggttc agcacaacaa
    27421 agctgaacaa caggcgaaag tggatgctga gaaactgctg gttgatttga aagccggcaa
    27481 aggtgcggaa gctatgcagg ctgccggtct gaaatttggc gagccgaaaa ccttaagccg
    27541 ttccggtcgt gacccgatta gccaggcggc gtttgcactg ccactgccag cgaaagacaa
    27601 accgagctac ggtatggcga ccgatatgca aggtaatgtg gttctgctgg cgctggatga
    27661 agtgaaacaa ggttcaatgc cggaagatca gaaaaaagcg atggtgcagg gtatcaccca
    27721 gaacaacgca caaatcgtct ttgaagctct gatgagtaac ctgcgtaaag aggcgaaaat
    27781 caaaattggc gatgcgttgg aacagcaata atcctgaagc cgcctcgcaa aaaaatgctt
    27841 tgcagctgta acaagaaaag gtcgctttcg cggccttttc catttctgaa cattgccatt
    27901 tgtttactgt tttcactgcc gttaaggtga ttccactgtt aacaaacaag gagaaaacag
    27961 tatgaaacac ggaattaaag cactgctcat taccctgtcc ctggcctgtg ccggaatgtc
    28021 tcatagcgcg ctggcggcag cttctgtggc gaaaccgacg gcggtagaaa ccaaagcgga
    28081 agctcctgca gcacaaaata aagcagcagt accggcgaaa gccagtgacg aagaaggctc
    28141 ccgggtcagc attaataatg ccagcgcgga agagctagcc cgcgcgatga atggcgttgg
    28201 cctgaagaaa gcgcaagcga ttgtcagtta tcgcgaagag tacggtccgt ttaaaacggt
    28261 ggaggatcta aagcaggtgc cggggatggg caattcgctg gtggaacgta atctggcggt
    28321 attaaccctg taattaattt gcatagtggc aattttttgc cagactgaag aggtcatacc
    28381 agttatgacc tctgtactta taacagtttt tataaaaaca acgtaaggtt attgcgctat
    28441 gcaaacacaa atcaaagttc gtggatatca tctcgacgtt taccagcacg taaacaacgc
    28501 ccgctacctt gaatttctcg aagaagcccg ctgggatggg ttggaaaata gcgacagttt
    28561 tcactggatg acggcccata acatcgcttt cgtggtggtg aatatcaata ttaactatcg
    28621 tcgtccggcg gtattaagcg acttgttaac cattacgagc cagttacagc aattaaacgg
    28681 taaaagcggc atcttaagcc aggtcattac actggagccg gaagggcagg tggtagcgga
    28741 tgcgcttatt acgtttgttt gtattgatct taaaacgcag aaagcattag ctctggaagg
    28801 ggaattgcgc gaaaagctgg agcagatggt taagtaaacg ttttgtggtg ccggatgctc
    28861 aagccgcatc cggcgacacc cggaataatt acttcaaccc ggttttctgc ttcatcgctg
    28921 ccatcaccgt cggtttatcg gccagataat gattcaaacc gttggcgcgt aaattacatg
    28981 ccgcacaatg accgcaaccg tcgcctttaa tgccgttata gcaggtcaac gtttcgttac
    29041 ggactaaatc cagtttgccg taataatctg ccagcgccca ggtttccgct ttatcaatcc
    29101 acatcagcgg cgtttcaaaa cgaatatctt tcgccatgcc caaactgacg gcatggttta
    29161 gtactttcac aaactcatcg cggcaatccg ggtagccgga gaaatccgtt tcgcagacgc
    29221 cggtaattac ggcttctgct tttacctgat acgcatatat tgccgccagc gtcaggaaca
    29281 aaatattacg ccctgggaca aacgtattcg ggatgccatc ggcttcaggt tcataatcag
    29341 gcaccggaat gctgtcacgc gtcaggctgc tgaccgccag ctcgttgagc agcgttacat
    29401 caagaacctt atgcgcgcgt gcccccagtt tcagcgccag ttcgcgtgcc acgtcgattt
    29461 ctgcgcgatg gcgctgaccg taatcgaacg tcacgcaatg gacttcatca tattgttgta
    29521 atgcctgcac cagacaggtg gtggaatcct ggcctccact gaacacaacg acagcacgtt
    29581 tcatagataa tcctgcttga acaataaaag cgttatggta acgcctgcga ttaacccgga
    29641 ccagctattc attgcgacgg cgcgggaagc caggcttcgg taaattcaaa ccagccgcgc
    29701 ggtgtcagtc gcacaccgtt cacgccggga ggggcactaa tgcgatagtg atagttgaac
    29761 agcggcgtca gcgtcgcatc tgtcattaac tggctaaaaa ccgctttcag ggcattaaat
    29821 cggttttcct catcaggcat tacttgcacc gcgtccagtg tcgattgtag atgtgcgtat
    29881 gctggagcgt cgaaaacatg tggccacagc ggatcgcagc gcagccattg ctccagagta
    29941 tattccggtg cttcgccaat taatctgtcg cccatcatga ggtctgcgtg tgccagtagg
    30001 gtcgtgtcgt cccagttttt tgcgttatga aaaataattg tgagttcaca gccttccgct
    30061 gccagtgtcg cctgtaggcg ttctgccatg gtatgaagtt ctatcgggag gtgataaacc
    30121 agcgtcaagg ttttcggtag tttgacttca tccggtactt gccaatgcgg aatagtccag
    30181 cctggcagta atgcatgact ggcggtgatc aggttttctc cgacttctaa cgtttgtaat
    30241 aaaccggatt gatgaataat ggagatcact tttcgcgcct gccagaggga gagtcgggga
    30301 cttttgcgca acgttaaata gcaaaaacct aaactgatgc cactactgac ctggctgacc
    30361 cgttgcagct cctccggttt gccgatggtg atttgcacgg gatgccgaca actggttccc
    30421 aaatcttttt cgaaaagcgg cggagttatc cagtactcaa ccgctttaag cagcggatga
    30481 cgtaaatggt aataatcatg gctttccagg cgcaccagtt cagctgtgaa ttgtgttaag
    30541 cgaaaaggac ccgtgccaat cagtgggaat tgcggatgcg ccagatggct gcaatagctc
    30601 gccagccggt gcgcaagcca gtaatcgggg cgatgtaaaa agaaggtcag acactgcgga
    30661 tgggtgacct caatacgctt cacgctaata aataattgat ccagtgctgg cagttgtaac
    30721 agcatcaata atcgctggtg taagtgtgag gcttttactg catcgccgtt atgccagtgt
    30781 agggttgaac gaagataaaa gtcccagcgt aacccgtcag cagaggtttc ccagtgatgc
    30841 gctaaatcgc caatcgggcg ctgggtatta ttatcgaagc gggtcaggcc ggaaaatatc
    30901 tgtccggcga gatgctgctc ggcacggccg ggcaaaaagc ccggttgtag cggttcgagc
    30961 gggcgatagt agggaatacg caacgtgggt gtatcgtttt gccattgtcc gcccataaac
    31021 ggctgtaaca gagtgcgcag ctcacctggg gccagttgcg ccagctccag cacatcttgc
    31081 tgctttccgg tttccagtgc cttttccatc atcgcattgc gtagcgattc tggcgtgacc
    31141 agaaagcgta attgtccgcg ctttccgcgt cctgactgcg cctgccactc cagccatccc
    31201 gcttcctgtg cctgacgcaa cagcgtacga acatggcgtt cgctgcaaaa acagcgttcg
    31261 gccagttcgc tgacggtgac ggtttgcggc tttccggcgg aaggttgcca cagacgttga
    31321 tactggttaa ggcggttgag cagtcgcata aaaacccgga acaatattat ttaactattc
    31381 actattactt ccgtatatat caggtgatac tcaatcacca ttaaccgtgt cacagagtgg
    31441 agaaagaaat ggctcgtctg gcagcatttg atatggatgg cactttattg atgcctgacc
    31501 atcatttagg tgagaaaacc ctctctactt tggcgcgact gcgtgaacgc gacattaccc
    31561 tcacttttgc cacggggcgt catgcgctgg agatgcagca tattctcggg gcgatatcgc
    31621 tggatgcgta tttgattacc ggcaacggaa cgcgcgtgca ttctctggaa ggtgaacttt
    31681 tacatcgtga tgatttacct gcggatgtcg cggagctggt gctgtatcag caatgggata
    31741 cccgagccag catgcatatt ttcaatgacg acggttggtt taccgggaaa gagatccctg
    31801 cgttgttgca ggcatttgtc tatagcggtt ttcgttatca aataatcgat gtcaaaaaaa
    31861 tgccactcgg cagcgtcacc aagatctgct tctgtggcga tcacgacgat cttacacgtt
    31921 tgcagatcca gctacacgaa gcattaggcg agcgtgcaca tttgtgtttt tccgccacgg
    31981 attgccttga agtgctgccg gtgggctgca ataaaggcgc tgcactgacg gtgctgaccc
    32041 aacatttagg tttatcgttg cgcgattgca tggcctttgg tgatgcgatg aacgatcgcg
    32101 aaatgttagg cagcgtcggt agcggattta ttatgggcaa tgcgatgccg caactgcgcg
    32161 cggagctccc gcatttaccg gtgattggac attgccgaaa tcaggctgtc tctcactatt
    32221 tgacgcactg gctggactat ccacatctac cttattcccc cgaataacga gatcccttcc
    32281 agcaccgggc aattgcccgg tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt
    32341 aaaccgcaca gaataaattg tcgcgatttc acctttaaaa tagaattaaa agagaaaaaa
    32401 attctccgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactacag
    32461 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccaccccc
    32521 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    32581 ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa aacgcaacat
    32641 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    32701 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    32761 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    32821 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    32881 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    32941 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    33001 aactggttcc accaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    33061 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
    33121 tcctgcgtta cgtctggcgc gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    33181 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    33241 gcacgggtga cctcatggct cgtgcgacta atgacgtcga tcgcgtcgtg tttgccgccg
    33301 gagaaggggt gctgacgctg gtggattcac tggtaatggg ctgcgctgtg ttgattatga
    33361 tgtctacgca aattagctgg cagttgacct tatttgccct gttaccgatg ccagtgatgg
    33421 cgatcatgat taagcgcaac ggcgatgctt tgcatgaacg ctttaagctg gcacaggcgg
    33481 cgttttccag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
    33541 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    33601 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
    33661 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
    33721 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    33781 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
    33841 gcgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    33901 gtggcgaact ggatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    33961 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
    34021 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    34081 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    34141 tggcggtagt tagccagacg ccattccttt tttctgacac cgtggcgaat aacatcgcgc
    34201 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    34261 atgacgatat tttgcgtcta ccgcaaggtt acgatacaga ggtgggcgag cgcggtgtga
    34321 tgctttccgg cgggcaaaaa cagcgtatct ccattgctcg tgcgttatta gtcaacgcgg
    34381 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcactgag caccagatcc
    34441 tgcataacct gcgtcagtgg gggcagggaa gaacggtaat catcagtgcc catcgccttt
    34501 ctgcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
    34561 gcaatcatga tgtgctggca caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    34621 aactggaggc ggcgctcgac gacgctccgg aaaatcgcga ggaggccgtc gatgcgtagt
    34681 tttagccaac tgtggccgac tctcaagcgc ctgttagcgt acggttcacc gtggcgtaaa
    34741 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcgg cggcagaagt cagtgggccg
    34801 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaagtg
    34861 gttgcagggc tggctgcggc gtatgttggg ctgcaactgt ttgccgccgg gctacattac
    34921 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    34981 gtgatggatg ctgcgttacg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    35041 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    35101 gcaactgtcc tgcgcagtgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc
    35161 gactggcgaa tggcgctggt ggcgataatg attttcccgg tggtgctggt ggtaatggtg
    35221 atataccagc gttacagcac gccaattgtc cgtcgtgtgc gcgcctatct ggcggatatc
    35281 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt tcgtcagcag
    35341 gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac actatatggc gaggatgcaa
    35401 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    35461 ctctgtggct tgttgatgct gtttggcttc tccgccagcg gcaccattga agtgggcgtg
    35521 ttgtatgcgt ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
    35581 cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga gctgatggac
    35641 ggaccgcgcc agcaatatgg caatgatgat cgcccgttac agagtggcac catcgaagtc
    35701 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    35761 gtgccttcgc gtaactttgt ggcgctggtc gggcataccg gcagtggtaa aagtaccctc
    35821 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    35881 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    35941 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tggggcggga tatctccgaa
    36001 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcacg cagtatgagc
    36061 gacgggattt atacgccgct gggcgagcag gggaataatc tctctgtcgg gcaaaagcaa
    36121 ctgctggcac tggcgcgcgt gctggtcgag acgccgcaaa tcctgatcct tgatgaggca
    36181 accgccagca ttgactccgg tactgaacag gcgattcaac atgctctggc ggcggtgcgt
    36241 gaacatacca cgctggtagt gattgctcac cgcttatcga ctattgttga tgccgacact
    36301 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg
    36361 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gcttgcagcc
    36421 agcgtgcgtg aagaagaatc attgagcgcc tgaatagcgc aatatctcat cgttggtgca
    36481 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc
    36541 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
    36601 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
    36661 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    36721 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    36781 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
    36841 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    36901 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    36961 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    37021 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
    37081 ccgataaagc cgacaatgcg tttatgatga tttgtactgc actggtgctg tttatgacta
    37141 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    37201 tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
    37261 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctgaaaa
    37321 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgta gcgtttcagg
    37381 gatcgtttgc ctgcattacc gtaggcttga tagttggggc gctggcggaa cgaatccgct
    37441 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt cctattgcgc
    37501 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
    37561 cggtggtgca tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
    37621 gcgtgggctt cggtaaagag gcgtttaaac cgcacaacct gccgatggtc ttcaccggga
    37681 ctgccattct ctatatcggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    37741 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    37801 ggatcttcgg tgaatgggcg ctgcgtggta agccttcact gctgggagcg tgttctggcg
    37861 cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggtgtt ggcggcgcgt
    37921 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttaccatg ctcaaacgct
    37981 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    38041 gctgtatcat gactgggatt tttgccgcca gctcgctggg cggcgtgggc ttcgctgaag
    38101 gtgtgacgat gggccatcag ttgctggtac agttggaaag cattgccatt acgattgtgt
    38161 ggtctggtgt ggtggcattt atcggctaca aactggcgga tctgacggtt ggtctgcgtg
    38221 tgccggaaga acaagagcga gaagggctgg acgtcaacag tcacggcgag aatgcctata
    38281 acgcataaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    38341 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagtacgcc gtcctgggta
    38401 taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg aggtgctctc cacgctatac
    38461 agcagccatt cattcaaatt aaacgggcga tggaaccaca tggaatggtc aatggtggca
    38521 atctgaatcc ccggttcgag aaaaccgata ccgtgcggct gtagagctac cggcaggaag
    38581 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    38641 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    38701 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttaagcact
    38761 ggcggcagca ggtgcgccag cgattgggcg atttgcgttt ccgaaggcag gccatcaggc
    38821 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttctggtgc ctggaaagag
    38881 gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    38941 ctgttaccgt cacgcagcgt ttcgacatca taaataatcg gcttcttact atcgccaggg
    39001 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt cagggacggt ctcttttgca
    39061 gcatacaagg cctgacccac gacctggccg ccaaatacct ggcgtaaacc taaatcttca
    39121 ctctggccgc gaaagagtcc ttcctcaatt ttttccagat ttaacaatgt cagtaaattt
    39181 tttagcgcct gactcatata actctccagt aacaaagctg ccgcagcaag ccaaagtgag
    39241 ttgagtataa cgcaaatttg ctactggtcc gatgggtgca atggtctgaa ttacgggcta
    39301 attacaggca gaaatgcgtg atgtgtgcca caattgttga cgttactatt ttgttaacca
    39361 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa atgaatgaaa
    39421 ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    39481 agcgcggata ttcagacgcc agccccggct gcaaatacgt ctatttcagc aacacaacaa
    39541 cctgctatcc agcaaccgaa tgtctccggt accgtctgga tccgtcagaa agtcgcactg
    39601 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcaccgtca
    39661 aaagtgctgg cgcagaaagc ggtgcgtact gaaggtaaac agtcaccatt cagctttgtt
    39721 ctgccattta acccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    39781 accgtgaatg acaaactggt atttattacc gataccgttc agccggtgat caaccagggg
    39841 ggaaccaaag ccgacctgac attggttccg gttcagcaaa ccgccgtgcc tgtccaggcc
    39901 agtggtggcg caacaactac cgtaccatcg acttcaccga cccaggtgaa tccatcttcg
    39961 gcagttcctg cccctacgca atactaagcg ttactaaccc ctttccatct ggagaggggt
    40021 tagtagttcc agcgatagcg ctgtaagtcg atttgcccgc ttcccgatac catcacacct
    40081 tctgccagta atgcctgtcg ttgacgctgt aaatccggtc cggttagcga aatggtgccg
    40141 tggcgattaa ccacccggtg ccagggcaag gtgctgcctt cggggagacg ctttaacaca
    40201 ccgccaacct ggcgcgcggc gcggggcgat cccgccagtt tcgccacatc accgtaagtg
    40261 gtgacatacc cttcgggaat agcggctacg atttgccaga cgcgttgggg aaatgaatct
    40321 tctttttcca tcttttcttc ctgagataat ttttcagcat aatctggaaa aacgcccgag
    40381 tgaagccgca ttgcgcaaga aaccagcatc tggcacgcaa tgggttgcaa ttagccgggg
    40441 cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt
    40501 tctcccgcaa cgctactctg tttaccaggt caggtccgga aggaagcagc caaggcagat
    40561 gacgcgtgtg ccgggatgta gctggcaggg cccccaccca tttctgcctc ccaccgtttc
    40621 gtcaaaaaat cccaacatga ctaaacttta accacaactg acgtcgcaag aattgtctgg
    40681 ctgcgcagta cgcttcggag gtatgtctga tgaagtatgt tgatggtttt gtggttgccg
    40741 ttcctgccga taaaaaggat gcctatcggg aaatggccgc taaggccgcg ccattgttta
    40801 aagagtttgg cgcgcttcgt attgtcgaat gctgggccag cgatgtaccg gatggcaaag
    40861 tgaccgattt tcgtatggcg gtgaaagcgg aagagaatga agaggttgtc tttagctgga
    40921 ttgaataccc ttcaaaagag gtccgcgacg cagctaatca aaagatgatg tcggacccac
    40981 ggatgaaaga gttcggcgag tccatgccgt ttgacggcaa gcgaatgatc tatggcggat
    41041 tcgagtcaat catcgacgaa taggtagcgt gacgggctgt gcggcgtgcc gtcagcccaa
    41101 aaattcacaa atgttgctcg gcccagcgta agaaatcttc tttcggtaat gccttgctgt
    41161 agagccagcc ctgaccgtag tgcacgccat gctggcgtag ccactcttct tgtttactgg
    41221 tttcgattcc ctccgctacc attttcagtt tcagtgtttt tgccatttcg atgatatgcg
    41281 gcgtgacatt tttatattcc agcgcatcaa cgaaagattt atcgatcttc agaatgtcga
    41341 catccagatc ctgtaaataa cttaaacttg aataccccgt accaaaatca tcaagataaa
    41401 tttcatggcc cgcctcccgg tagcgagaaa ttatcggggc gctggttttc ggatcggcaa
    41461 actcgcgttc agtgagttca agcgcgatct gtctgggatt aacctgatag tgattgatca
    41521 tttcacgcag caattgcggg attttttccg aggtgagcac ggtggattca agattgatcg
    41581 aaatatgctg ctgtggatgc tgacgcagcc agtcgcccat atcttcaaag acgcttctta
    41641 taatcagtag cgtcaatggc tcagaaaggc ccgtttgctg tgccagcgga ataaaactat
    41701 ctggtgacaa ccaactaccg tctgtctgcg gccagcgcgc cagtgcctca gcaccgacaa
    41761 ttttgccatt ggctaaggag acaatcggct gatagtgcac gcaaatatca cgattttcga
    41821 tagcatcctg cagccgatga tgcggtgact ggatgcggcg caaaatgcgc agcacaaaca
    41881 tcgctgccag caggccaatc accaacccga gcggtaacca aataaagact tgccgatgcc
    41941 agcctttctg caacattttc gatgaagccc aggtgataat tgaaatattc atctccggga
    42001 agggcaggat atcgtaaatg attccattat tttcgatatg ctcaccgggt gttttttgta
    42061 gcctggtaat aattccctga gcaatttcat cgctgctggt tatgacaacg ttatgggcat
    42121 tgccaataat ggcggcatca atttgccatg agctataggg aatgacatca ataaaggaag
    42181 cggggtcgat catgacgaca taatgtgccg ttcccatggc gaccatgtaa cggataatgc
    42241 ctaaatcgtt atgtgatgtt aaccagacac gataaccatc tttcgaaatt ttacctggct
    42301 cggggaaggt atcgggcgga ctttcatgct ccagagacga acactgggga acgttgttat
    42361 cgatataaac cacttcctga atatagcgat aactgtaaga tacccgacgc atttccatga
    42421 gatgggcttc actacagtca gcgccttgcc atctttccag ctcttgcagc gcatctttcc
    42481 cttgtgtcgc caccttattg gcccgaatag cgacgcggga ggaataggta tccagctctt
    42541 caataaacga tgtttctacc tgctgatggg ccagccagat gcttaagccg acaggcagca
    42601 atactgaaag aatcagtact cccgaaataa ggccgaccag atgtcgtgtt ctcaccagca
    42661 tatccttatt aacacagtgt agggaaagta tatctgattg tggaaaaagg ctggcaggag
    42721 ataaggaggt agagctggta tttcatgaag tgatgggtgg aaacgatcgt tgtcatttga
    42781 ccaggcgcag agctttacgc ctggtatgta atcctaactt aagatgttga ttcggcgcga
    42841 gcgagggtaa aaatatcgta aaaagacaat tcacctttac ggacgatcat tttttgcagt
    42901 tgttccttat gaacatccgc gacacgcgga gagttcaaga tggcttcaac cagcgcggca
    42961 ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa cgcggaaatc caacacgtcg
    43021 atatcttcat agcggtagcg tgggtagaca tcgaaaaaga agaaaccatt aagcaggata
    43081 aataatacaa ccagtagcca gacagagcct gaaagcgttt cggactgcaa catcaccgcc
    43141 agggtggcaa aaaaagcaac gtacataccg ataaacagcc ccggatgttt acggataaaa
    43201 ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg tattcagaga ctcaatcgtt
    43261 tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc gtggtatctg caactttcca
    43321 gggaacaccc tattttaacg tgggaatagt agagggaaag taacagatct gccacaattt
    43381 tgcataacag ttgcgaaaaa ccgattacaa ttttttaatt attctggctg gattaccgcc
    43441 cacgacaacg ttgtccggga catcttttgt gacaactgca cctgaggcta ccacgacgtt
    43501 atcaccaatg gtcacaccag ggttaatgac cgcgcgtccg ccaatccaga cgttattacc
    43561 gatggtgacg ggtttcccca gttcagcacc gctattacgt gctacagggt cgatgggatg
    43621 tgttgccgtg tagatatgaa cgcctagtgc caacatacag ttatcaccga tgcgaatagg
    43681 gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa ttattaccga gaaaaatgtt
    43741 atagccatag tcacagcgaa acgttggctc aatataagcc tctgtcacct gaccgaatag
    43801 atcagcgaga atttgctggc gtaatgtgtg ctcttccgcc agggaatgat tgtatcggtg
    43861 aataagctga cgagcgcgca ggcgatcgcg agataacgtc tcatctgccg agcgatacaa
    43921 ctcaccagca atcatctttt ctttttctgt gctcattact gacctccggt tgctgaaaat
    43981 gcggcaacac agtaagacga aaagtcagtg aagagaacgt tcccattaca tctgtgtgat
    44041 aaagatcaca tagggtatgt cttcatggcg aaaaagtata aaattcttaa taaacagccg
    44101 gttatagctc cgaaagcgaa ttatttatta gcgaataaat ttccatactg aggaagggat
    44161 cttgtcgtac agtttattca tggtcaattc ggcgaggcgg tgatctgcgg ctgagtaaaa
    44221 taccgccagt tcattatctg ataattcgta tttatttttc tcgataacac gctccagcgt
    44281 gtcaattgtc tggcaacgac gtaaacgcat taaataatcg gttttcgtta aaggtttttc
    44341 ggacatactt ctacccatgg ttgtaataat tctaacaaga taaactcgca ggattctctt
    44401 tcgtcgcatt gacaaaacaa cggaatagtc gattacctga cttccgccat ttctgaagat
    44461 cctgcatatt aataccataa ctactgaaca acataaaggt gtcatccaga tattcgtcga
    44521 tctgctcaat gagcttattg tcttcattat acttaatttt gtaattaagt gcgaaggtcg
    44581 caatatgctc aatcagttca ttcaactgga ggttgatcgc cgaggttggg tcgttaaccc
    44641 agccatgatt gctttcttca aggtttgcaa ggcagtcatg atacagagtt tcacagagaa
    44701 acttaagctg tgcgatatca tgtcttttgg gtgagtattc atccataacg cgtccccttc
    44761 ttagcggttg aactaacgga cacctttcgg gatggaaaaa acttactgac ctggacttgc
    44821 cctctcgttt gttagcttaa ctataagcca ctctttgcag gttttcatcg cattattacg
    44881 aaaaattaca attaggaagg aagcgggaca gggctcactt tttcatacta gcaccaaccc
    44941 gcagcaggta aaagcagttt gctcgaactg ttcataacat ttcaggatta tcgccagatg
    45001 aaattggtga cactaatacc aggattgctc tgaatatgac gtaataaccg aggaatgaat
    45061 aaagaattac cgcaaa
//
LOCUS       DAZFTN010000021        45007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFTN010000021 DAZFTN010000000
VERSION     DAZFTN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..1708)
                     /gene="actP"
                     /locus_tag="ACHKV2_001391"
     CDS             complement(59..1708)
                     /gene="actP"
                     /locus_tag="ACHKV2_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO6939310.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1705..2019)
                     /gene="yjcH"
                     /locus_tag="ACHKV2_001392"
     CDS             complement(1705..2019)
                     /gene="yjcH"
                     /locus_tag="ACHKV2_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO6939311.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2220..4178)
                     /gene="acs"
                     /locus_tag="ACHKV2_001393"
     CDS             complement(2220..4178)
                     /gene="acs"
                     /locus_tag="ACHKV2_001393"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO6939312.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4571..6007
                     /gene="nrfA"
                     /locus_tag="ACHKV2_001394"
     CDS             4571..6007
                     /gene="nrfA"
                     /locus_tag="ACHKV2_001394"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO6939313.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6052..6618
                     /gene="nrfB"
                     /locus_tag="ACHKV2_001395"
     CDS             6052..6618
                     /gene="nrfB"
                     /locus_tag="ACHKV2_001395"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO6939314.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6615..7286
                     /gene="nrfC"
                     /locus_tag="ACHKV2_001396"
     CDS             6615..7286
                     /gene="nrfC"
                     /locus_tag="ACHKV2_001396"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO6939315.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7283..8239
                     /gene="nrfD"
                     /locus_tag="ACHKV2_001397"
     CDS             7283..8239
                     /gene="nrfD"
                     /locus_tag="ACHKV2_001397"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO6939316.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8259..9977
                     /gene="nrfE"
                     /locus_tag="ACHKV2_001398"
     CDS             8259..9977
                     /gene="nrfE"
                     /locus_tag="ACHKV2_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO6939317.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9970..10353
                     /gene="nrfF"
                     /locus_tag="ACHKV2_001399"
     CDS             9970..10353
                     /gene="nrfF"
                     /locus_tag="ACHKV2_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO6939318.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10350..10946
                     /gene="nrfG"
                     /locus_tag="ACHKV2_001400"
     CDS             10350..10946
                     /gene="nrfG"
                     /locus_tag="ACHKV2_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO6939319.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11288..12601
                     /gene="gltP"
                     /locus_tag="ACHKV2_001401"
     CDS             11288..12601
                     /gene="gltP"
                     /locus_tag="ACHKV2_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO6939320.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12779..13468)
                     /gene="yjcO"
                     /locus_tag="ACHKV2_001402"
     CDS             complement(12779..13468)
                     /gene="yjcO"
                     /locus_tag="ACHKV2_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO6939321.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13562..15709)
                     /gene="fdhF"
                     /locus_tag="ACHKV2_001403"
     CDS             complement(13562..15709)
                     /gene="fdhF"
                     /locus_tag="ACHKV2_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15290..15292),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO6939322.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15907..17373)
                     /gene="mdtP"
                     /locus_tag="ACHKV2_001404"
     CDS             complement(15907..17373)
                     /gene="mdtP"
                     /locus_tag="ACHKV2_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO6939323.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17370..19421)
                     /gene="mdtO"
                     /locus_tag="ACHKV2_001405"
     CDS             complement(17370..19421)
                     /gene="mdtO"
                     /locus_tag="ACHKV2_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO6939324.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19421..20452)
                     /gene="mdtN"
                     /locus_tag="ACHKV2_001406"
     CDS             complement(19421..20452)
                     /gene="mdtN"
                     /locus_tag="ACHKV2_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO6939325.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20471..20746)
                     /gene="ytcA"
                     /locus_tag="ACHKV2_001407"
     CDS             complement(20471..20746)
                     /gene="ytcA"
                     /locus_tag="ACHKV2_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO6939326.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20955..22940)
                     /gene="yjcS"
                     /locus_tag="ACHKV2_001408"
     CDS             complement(20955..22940)
                     /gene="yjcS"
                     /locus_tag="ACHKV2_001408"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO6939327.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARIINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23231..23938
                     /locus_tag="ACHKV2_001409"
     CDS             23231..23938
                     /locus_tag="ACHKV2_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6939328.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            complement(23935..24936)
                     /locus_tag="ACHKV2_001410"
     CDS             complement(23935..24936)
                     /locus_tag="ACHKV2_001410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6939329.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(24933..25793)
                     /locus_tag="ACHKV2_001411"
     CDS             complement(24933..25793)
                     /locus_tag="ACHKV2_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO6939330.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            complement(25808..26491)
                     /locus_tag="ACHKV2_001412"
     CDS             complement(25808..26491)
                     /locus_tag="ACHKV2_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO6939331.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            complement(26546..27487)
                     /locus_tag="ACHKV2_001413"
     CDS             complement(26546..27487)
                     /locus_tag="ACHKV2_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6939332.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(27506..28480)
                     /locus_tag="ACHKV2_001414"
     CDS             complement(27506..28480)
                     /locus_tag="ACHKV2_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6939333.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            complement(28477..29997)
                     /locus_tag="ACHKV2_001415"
     CDS             complement(28477..29997)
                     /locus_tag="ACHKV2_001415"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6939334.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLIDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30111..32390
                     /locus_tag="ACHKV2_001416"
     CDS             30111..32390
                     /locus_tag="ACHKV2_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6939335.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVAT
                     LSGQRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAH
                     GEADVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLE
                     EALLHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGA
                     LLTQRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDI
                     SQLENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQ
                     AVKSQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRAL
                     TAAKNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDL
                     LAASAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVA
                     LLRKPFTRAQLAQALGQEN"
     gene            32459..32788
                     /gene="yjdP"
                     /locus_tag="ACHKV2_001417"
     CDS             32459..32788
                     /gene="yjdP"
                     /locus_tag="ACHKV2_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO6939336.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(32865..33623)
                     /gene="phnP"
                     /locus_tag="ACHKV2_001418"
     CDS             complement(32865..33623)
                     /gene="phnP"
                     /locus_tag="ACHKV2_001418"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO6939337.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(33625..34060)
                     /gene="phnO"
                     /locus_tag="ACHKV2_001419"
                     /pseudo
     CDS             complement(33625..34060)
                     /gene="phnO"
                     /locus_tag="ACHKV2_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            complement(34047..34604)
                     /gene="phnN"
                     /locus_tag="ACHKV2_001420"
     CDS             complement(34047..34604)
                     /gene="phnN"
                     /locus_tag="ACHKV2_001420"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO6939338.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(34604..35740)
                     /gene="phnM"
                     /locus_tag="ACHKV2_001421"
     CDS             complement(34604..35740)
                     /gene="phnM"
                     /locus_tag="ACHKV2_001421"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO6939339.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(35737..36417)
                     /gene="phnL"
                     /locus_tag="ACHKV2_001422"
     CDS             complement(35737..36417)
                     /gene="phnL"
                     /locus_tag="ACHKV2_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO6939340.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(36528..37286)
                     /gene="phnK"
                     /locus_tag="ACHKV2_001423"
     CDS             complement(36528..37286)
                     /gene="phnK"
                     /locus_tag="ACHKV2_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO6939341.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(37283..38128)
                     /gene="phnJ"
                     /locus_tag="ACHKV2_001424"
     CDS             complement(37283..38128)
                     /gene="phnJ"
                     /locus_tag="ACHKV2_001424"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO6939342.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(38121..39185)
                     /gene="phnI"
                     /locus_tag="ACHKV2_001425"
     CDS             complement(38121..39185)
                     /gene="phnI"
                     /locus_tag="ACHKV2_001425"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO6939343.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(39185..39769)
                     /gene="phnH"
                     /locus_tag="ACHKV2_001426"
     CDS             complement(39185..39769)
                     /gene="phnH"
                     /locus_tag="ACHKV2_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO6939344.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            complement(39766..40218)
                     /gene="phnG"
                     /locus_tag="ACHKV2_001427"
     CDS             complement(39766..40218)
                     /gene="phnG"
                     /locus_tag="ACHKV2_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO6939345.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(40219..40944)
                     /gene="phnF"
                     /locus_tag="ACHKV2_001428"
     CDS             complement(40219..40944)
                     /gene="phnF"
                     /locus_tag="ACHKV2_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO6939346.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(40965..41744)
                     /gene="phnE"
                     /locus_tag="ACHKV2_001429"
     CDS             complement(40965..41744)
                     /gene="phnE"
                     /locus_tag="ACHKV2_001429"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO6939347.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(41850..42866)
                     /gene="phnD"
                     /locus_tag="ACHKV2_001430"
     CDS             complement(41850..42866)
                     /gene="phnD"
                     /locus_tag="ACHKV2_001430"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6939348.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            complement(42891..43679)
                     /gene="phnC"
                     /locus_tag="ACHKV2_001431"
     CDS             complement(42891..43679)
                     /gene="phnC"
                     /locus_tag="ACHKV2_001431"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO6939349.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(43812..44255)
                     /gene="yjdN"
                     /locus_tag="ACHKV2_001432"
     CDS             complement(43812..44255)
                     /gene="yjdN"
                     /locus_tag="ACHKV2_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO6939350.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(44514..44849)
                     /gene="yjdM"
                     /locus_tag="ACHKV2_001433"
     CDS             complement(44514..44849)
                     /gene="yjdM"
                     /locus_tag="ACHKV2_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO6939351.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         9687 a        12267 c        12728 g        10325 t
ORIGIN      
        1 tgatgcattt gggagaggaa gagtattccc cggtcagacg accggggaag gggagagatt
       61 aatgcgcgcg gccttgctca acgccaaagc cggtctggga gcggataaac tgcgcgcgga
      121 acagttcacg ctcacgcgcg ccttccgctg agttatcggt tgccgagaag aaccagatgc
      181 cgaggaatgc cacggtgata gagaacagcg ccgggtattc atacgggaag atggcttttt
      241 cgtgaccgag gatctgtacc caaatcgtcg ggccgaggat catcagtacc actgcggtaa
      301 tcagccccag ccagccaccc atcatcgcgc cacgcgtggt cagtttcgac cagtacatag
      361 aaagcagaat gatcgggaag ttacagctcg ccgcgatggc aaacgccagc cccaccataa
      421 aggcgatgtt ctgattctca aacagcacgc cgaggataat cgcaatcaca cccaaaatca
      481 ggacggtgat tttcgatacc cgcagctctt cacgttcggt cgcgcctttt ttgaagacgt
      541 tagcgtacag gtcatgcgaa accgctgatg cgcccgccag cgtcagaccc gcaaccaccg
      601 cgaggatagt ggcgaaagca accgctgaaa taaagccgag gaacaggttg ccgcccaccg
      661 cattggccag gtgaactgct gccatgttgt taccaccaat cagatggccc gccgcgtctt
      721 tatattccgg attcgcaccg accagcatga tcgcgccgaa gccgataata aaggtcagga
      781 tatagaagta gcccataaat ccggtagcgt agaacacgct tttacgcgct tcgcgggcat
      841 cgctgaccgt aaagaagcgc atcagaatgt gcggcaagcc agccgtacca aacatcagtc
      901 ctagccccag agagagtgcg gagatcggat ctttcaccag tccgcccggt ttcatgatgt
      961 cgacaccttt cgggtgtacc gccatcgctt cgctgaacag attgttgaag ctaaagccga
     1021 cgtgtttcat caccataaag gccataaagc tggcaccgaa cagcaacagc acagctttga
     1081 taatctgtac ccaggtggtc gccagcatgc cgccgaacag gacgtacatc atcatcagca
     1141 cgccgaccag caccactgca atgtgatagt taaggccaaa cagcagctcg atcagtttac
     1201 ctgcacccac catctgggcg ataaggtaaa gcgccaccac caccagagaa ccacaggccg
     1261 aaagaatacg aatcggccct tgtttcagac gataagaggc tacatcggca aaggtgtaac
     1321 gccccaggtt acgcagacgt tcggcaatca ggaacaaaat gatcggccag cccaccagga
     1381 agcccagcga gtaaattaag ccgtcatagc cggaggtaaa caccagtgcg gagatcccca
     1441 aaaatgaggc ggcggacata tagtcaccgg caatcgccag cccgttctga aaaccggtga
     1501 tattgccgcc tgcggtgtaa tagtcattac gcgaacgtac gcgttttgac gcccagtagg
     1561 taatgccgag cgtaaacacg acgaaaatca ggaacataat aatcgcctgc cagttcgttg
     1621 gctggcgctc tacggccccg ctaatagcat ccgcggcgtt agctgcgaaa gggagtgtgg
     1681 cggcaagcgc cgtcagaact cttttcatga tgcttgtacc tcatgcagga cttcattatt
     1741 aagacggtcg aattcgccgt tcgcccgcca gatgtagata ccagtgagaa caaaggagat
     1801 cactattacc ccaacaccga ctggaatacc gcgtgtgacg ctggtgttcg gattcagcgg
     1861 ggtgcccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat aaactgccag
     1921 cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact ccctgaaatg
     1981 cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc cagaggtaat
     2041 gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg ggtaaccctt
     2101 gccgaatgta ggccggataa ggcgttatac gccgcatccg gcaatcaatg cctgatgcga
     2161 cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc aattgtgggt
     2221 tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac gccaggatcg
     2281 gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt gcgcagaata
     2341 cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg cagcacgtct
     2401 ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc gtacagttct
     2461 ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg acctttaata
     2521 ttgtgcggaa taccgacgac agcggcttcg gcgattttcg gatgcgccac cagcgccgac
     2581 tcaatctccg ccgttcccag acggtgaccg gagacgttca gcacatcgtc cacacgcccg
     2641 gtgatccagt aatagctatc ttcatcacga cgcgcgccgt cgccgctgaa atacatattt
     2701 ttgaaggtgg aaaaataggt ctgctcaaaa cgttcgtgat cgccaaacag cgtacgcgcc
     2761 tgacccggcc aggagtcggt gatcaccagg ctaccttcgg tagccccttc cagcgggtta
     2821 ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc cgaaccggct
     2881 ttcagctcgg tagcgccagg cagcggggtg atcatgaaac cgccggtttc ggtctgccac
     2941 caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca ctcccacgct
     3001 tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg gtcggtgcct
     3061 tcgatcgctt tatcgccttc cgccatcagc gcgcgaatcg ccgtgggcgc ggtatagaga
     3121 atattgacct gatgcttgtc caccacctgt gccatacggg caggcgtcgg ccagttcggt
     3181 acgccttcaa acatcagcgt ggtcgcgccg caggccagcg ggccgtacag caaataactg
     3241 tgtccggtca cccagcccac atcggcggtg caccagtaga tatcgcccgg atgataatca
     3301 aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt gtgcagtacg
     3361 ccttttggtt ttccggtaga accggaggta tagagaataa acagcggatc ttcggcgttc
     3421 atctcttccg cctggtgctg atcgctggct tgctcaacct ggtcgtgcca ccacaggtcg
     3481 cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac atgctctacg
     3541 ctggtgacgt tcgggttttt tagtgcgtca tcaacgtttt tcttcagcgg aatactacgc
     3601 ccggcgcgca cgccttcgtc ggaagtgatc accagtcgtg agttggaatc gataatgcgc
     3661 ccggcaaccg cttccggcga gaagccaccg aaaattaccg aatgcaccgc gccaatacgg
     3721 gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat cgccaccaca
     3781 tcaccttttt taatgcccag cttgagcagg gtattggcga agcggcagac gtcgcggtgc
     3841 agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca gatgatggcg
     3901 gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag attcagcgtg
     3961 ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt tttcaccttc
     4021 tggtacggtt tgatccagtc gagaattttt ccctgttcgc cccagaaggt atcaggtgcg
     4081 ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca acggtctgcg
     4141 atgttggcag gaatggtgtg tttgtgaatt tgactcatgc ttttgttctc cttgtaggat
     4201 gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg tttagtattt
     4261 gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa tttttgaagg
     4321 tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcatgc tgttgaaaaa
     4381 gaggaagata ctgactaact ctaaagtggt attttacatg cacttacaat tgattaaaga
     4441 caacattcac agtgtggtta tttgttacac ttaggggcga gcaatgtcat gacagtgtag
     4501 gtgcggttac tgtcgtgaaa aacaataaaa accgccattg caacaatggc gcaattcgga
     4561 tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct tattgattcc
     4621 ttttttcttt ttcacttctg ttcacgctga acaaacggct gctcccgcaa aacctgtaac
     4681 tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc tctcctggaa
     4741 agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac ggctggtgat
     4801 cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac atgcctttgc
     4861 tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg aagatggtcc
     4921 gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga tccagaaaga
     4981 cggcgaagat ggctacttcc acggtaaatg ggcgcgcggc ggcccggaaa tcgtcaacaa
     5041 cttaggttgt gccgattgcc ataacaccgc ctctccagag ttcgccaaag gcaaaccgga
     5101 gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta aaccttttga
     5161 gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg tggagtatta
     5221 cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga aagtcgaaaa
     5281 tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc tgtcgaaaac
     5341 gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca ttcacggtaa
     5401 aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg aaggcaaact
     5461 ctacaccgac cataaaattg gtaatccgtt tgataacttc gcccagactt gtgcgaactg
     5521 ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc agtcgattaa
     5581 cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag cgaaagcggc
     5641 gctggacgca ggtgcgacgg aagccgaaat gaagccgatt caggacgata tccgtcatgc
     5701 ccagtggcgt tgggatctgg cgatcgcttc ccacggcatt catatgcacg caccggaaga
     5761 aggtctgagg atgctcggta cggcgatgga taaagcggcg gatgcacgca ccaaactggc
     5821 gcgcctgctg gcgactaaag gcatcaccca tgaaatccag atcccggata tctcgaccaa
     5881 agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg agaagcagga
     5941 cttcatcaaa acggtgatcc cgcagtggga agagcaggca cgtaaaaacg gtctgttaag
     6001 ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa tatgagcgta
     6061 ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag cctgaacgga
     6121 attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac ccagcagcgt
     6181 aacccggatg ccgcctgtct ggactgtcat aagccagata ccgaaggtat gcatggcaaa
     6241 catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa ctgtcacggc
     6301 cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa cgagccgatg
     6361 tacaaggttg gagagcagaa cagcgtctgt atgtcctgcc atctgccgga acagttgcaa
     6421 aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag ctgccattcc
     6481 ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat taagatttgc
     6541 gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc tgttccgttg
     6601 cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc ggcgtgctgg
     6661 cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc aatggggtgc
     6721 gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt atggatgctt
     6781 gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt cgtagcgagc
     6841 cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc cagcactgcg
     6901 atcatgcgcc gtgcgttgac gtctgcccga ccggtgcatc ttttcgcgat gctgccagtg
     6961 ggattgtcga tgttaatccg gatctctgcg tcggttgtca gtactgcatc gccgcctgtc
     7021 cgtaccgcgt gcgctttatt catccggtca cgaagacagc ggacaaatgc gatttctgcc
     7081 gtaagaccaa tttgcaggcc ggtaagcttc ccgcgtgcgt tgaggcttgc ccgacgaagg
     7141 cgctgacgtt tggcaatctg gacgatccca acagtgagat ttcgcaactg ctgcgccaga
     7201 agcccactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac cgcgtaccgt
     7261 ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg aatcgctggt
     7321 gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg gtctggtgac
     7381 gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca gcacgttgct
     7441 gcgcaccacg ctgattgtcg gacctggcgc ggtgatcctc ggtctgttga gcctcgtctt
     7501 ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt ttacttcggt
     7561 gatgtcgatg ggggtgatgc tgtttcagct ttacatggtg gtgctggtgc tgtggctggc
     7621 gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga agctggggat
     7681 cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa cattgatgct
     7741 ggtgctggcg gtgttgttgg gggcttatac cggttttctg ctgtcggcgc tgaaatcgta
     7801 tccgttcctt aataacccga tcctgccggt gctgttcctc ttctccggca tctcgtccgg
     7861 tgcggcggtg gcactgatcg ccatggcgat acgccaacgc agtaacccgc attccacgga
     7921 agcgcagttt gtacaccgta tggaaatccc cgtggtatgg ggtgaaatct tcctgctggc
     7981 ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gtgcgtgcgc tggtggcggc
     8041 attgggcggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc tggggctgat
     8101 tgtgccaatg ttgttcaaac cgtgggtcaa tcgcagttcc ggcattcctg ccgtgctggc
     8161 ggcgtgtggg gccagtctgg tcggcgtgtt gatgttgcga tttttcattc tctacgccgg
     8221 gcagttaacg gtggcgtaag ccagaacaga ggtggtttct ggacgtattc cttcctgaag
     8281 tcggttttct ggcgctgttg ttaagtctcg gggtcaacgt gttgaccccg ttgacggcct
     8341 ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc ggcattctgg
     8401 cacagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt ctcatcagcg
     8461 atttctcggt catttatgtc gcccaacata gctacagctt gctgtcgtgg aaactcaaac
     8521 tggcggcggt gtggggcggt catgaaggtt cgctgctgct ttgggtgctg ctgctttccg
     8581 cctggagcgc gctgtttgcc tggcaatatc ggcagcaaac cgatccgcta tttccgctga
     8641 cgctagccgt tttatctctc atgctcgccg cactgctact gtttgtagtg ctgtggtccg
     8701 atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat ccgatgctgc
     8761 aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc ggtttgatgg
     8821 tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatggt gcctgcgccc
     8881 gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aactgcgggg atcatcctcg
     8941 gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg gatccggtgg
     9001 aaaacgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt ttatccctga
     9061 cacgccagcg ggggattttt cgccactggt cgctgttgtt ggcgatagtt actctgatgc
     9121 tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt catgcgttcg
     9181 ctctggataa cgttcgcgct gtgccgttgt tcagcctgtt tgcactgatt agccttgcgt
     9241 ctctggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt ttttcggggt
     9301 tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg ctactgatcg
     9361 tgctggtggg aacgctttat ccgatgattt acggcctgct gggctgggga cgtctctccg
     9421 ttggcgcacc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg ttggtggtga
     9481 ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg ctggcgcatg
     9541 cgggcatact gttatttgcc gcagggatcg ttgtctccag cgtcagccgt caggagatca
     9601 gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc cgttttgagc
     9661 gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg gcgctgtttg
     9721 accatcagca acgcattggt gaattaacgc cggagcggcg tttttacgaa gctcgtcgtc
     9781 agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat gcggtcatgg
     9841 gggagaaaac tggagcggat cgttacgctt ttcgcttgta tgtacaaagc ggtgtgcgct
     9901 ggatctgggg gggaggattg ttgatgattg cgggcgcatt gttaagcgga tggcggggga
     9961 agaagcgcga tgtataaagg gcttctcacg ctactgctgt tatttacctg ttttgcgcgc
    10021 gctcaggttg tagacacctg gcaattcgcc aatccgcaac aacagcaaca ggcgttaaat
    10081 attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactgga atccaacgcg
    10141 ccggtggcag tcagtatgcg ccatcaggtt tacagcatgg tggcggaggg gaaaagtgaa
    10201 gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta taacccaccg
    10261 ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttact gctgatggca
    10321 ctgatcctct ggcgagtgag ggcgaagcga tgaaacagcc tcaaataccg gtgaaaatgc
    10381 tgacaacact cacgatattg atggtattcc tctgtatcgg tagttatctg ttaagcccaa
    10441 aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat cagtttgcca
    10501 gccagcaaaa cccggaagcg cagcttcagg cattgcagga taaaatccgc gctaatccac
    10561 aaaacagcga acagtgggcg ttactgggcg agtactatct gtggcaaaac gattacagca
    10621 attcgctgct ggcgtaccgt caggcgttgc aactacgtgg tgagaacgct gaactgtatg
    10681 cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc cagactcgcg
    10741 caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg atgctgctgg
    10801 cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg caaaaagtga
    10861 tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt aatatggcga
    10921 aattgttgca gcggagatcg gattaatcat ctggtgatga agattatgtt attgagctgc
    10981 ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat tattgcagat
    11041 taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc ataaactcgc
    11101 gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca acataccgcg
    11161 caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca caattcatgc
    11221 cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca ttgaggaagt
    11281 cattcatatg aaaaatataa aattcagcct ggcctggcag attctgtttg ctatggtgct
    11341 gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact ggctggtcgt
    11401 caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga ttgttgtgcc
    11461 gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca aacagctcgg
    11521 gcgtattggc gcgaaaacca ttatctactt cgaggtgatc accaccgttg ccatcatttt
    11581 ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt cgcagctggc
    11641 gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca gttcccacgg
    11701 cattatgggc acgattttgt cgctggtgcc gacgaatatt gtggcgtcga tggcgaaagg
    11761 cgaaatgttg ccgatcatct tcttctcggt gctgtttggt ctggggcttt cctccctgcc
    11821 cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa ccatgtttaa
    11881 agtgactcac atggtgatgc gttatgcgcc ggtgggtgtg tttgcgctga ttgcagtgac
    11941 ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc tgctggtgca
    12001 tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc tatgcgggtt
    12061 aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt actccactgc
    12121 cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg gagcaccggc
    12181 gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg gttcgacgct
    12241 gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc tgtccatctg
    12301 gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg ctggcgtgcc
    12361 gggcgtgtcg tttgtggtgt tgctggcaac gctgggtagc gtaggtattc cgctggaagg
    12421 tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg cactgaacgt
    12481 ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat ttgaccgcaa
    12541 aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg cggatcaata
    12601 attgaagatt gccggggata tcccccggca atgtgtggat gcctgatgcg acgctggtgc
    12661 gtcttatcag gcctacgtca gacagcgcta gcgtgatttt gtaggtctga taaggcgttt
    12721 acgctgcatc cgacatcaac gcctgatgcg acgcttaacg cgtcttatca ggcctacctt
    12781 aaccgttagt taatttttca aactcttcac acccggtatc aaacccttcc atacagctca
    12841 ggttcaacca gtgcagcgcc ttttgcttgt tcttctcgat aaaaccttct tcaccgttta
    12901 agaacatcat tcccgcccag tactcggaat aaccggttcg ggaaattgca gagctgcgtt
    12961 tgaaatacca ggttgccttg tcatcgtcgg ccttaatgcc cacgccgttg gcgtaaatca
    13021 aaccaagcag catttgggca tcgacggcag agtcgttctc cagatcttcc gaggcgtttt
    13081 ctaacagcga aatcgccttt ggataatccg gtttacccgc ttgagtattt accagaatat
    13141 gcgccagcgt tacttcacct tctttactgc ccgcttgcgc cgctttctct gcaagaactt
    13201 ttgcttgtgg atagtccaga ctgaccggat tggtgatttt gatctgcgcc aacagcgcgc
    13261 aggcatcagc atcaccgctg tcggcggctt tctgtgccca atattcggct ttgctcaaat
    13321 cgccggagct aaaccagctg tcggcaagaa aatattgtgc gcgtcggtcc ccggcctctg
    13381 ctgcctttaa atactggctg ccaggctcgg agtcgttggc gtgggcaaag aatgtcaaaa
    13441 acaacatcaa tgcgataatt tttttcattt tgataatacg attgggtagg gagtaaccag
    13501 tataaagagt tcgaatggat aaaaaaacag cctccgaaag gaggctgtag aaaggactgt
    13561 attacgccag tgccgcttcg cgcaggcgag ttttcaactt gttgtactcg tcaatcacgt
    13621 actgctcggc ggcgcgctga tcggcgatcg gctcgacgcg cacggcgcag tatttgtatt
    13681 ccggcgtttt cgtaatcggg cttaagtttt cggtaaccag ctcgttacag gcaccaatcc
    13741 accactggta ggtcatgtaa atcgcccctt tgttcggacg atcgctgacc tgcgcacggg
    13801 tgataatttt gcctttacgc gagtgcaccc aaaccaatgc ctcatcttca atactcagac
    13861 gtttggcgtc ttcggtattg atttgtgcgt agccaggttc atcagccagc gccgccagtg
    13921 ccgcacagtt accggtcatc gaacggcaag agtagtgacc aacttcacgc accgttgaca
    13981 gtaccatcgg gtactcgtcg gtgagtttgt cgattggcgc tacccagtcg caggtgaaga
    14041 actgcgccag accgttcggg gtgtcaaact tctctttaaa cagataagaa gtcccctgat
    14101 cggcatctga agtatcgcgg caaggccact gaatgaagcc cagttcgccc attttctcgt
    14161 aagtcgcacc gtagaaatcc gggcacagat gacgcaactc atcccagatc tcctgggtgt
    14221 tgttgtagtg catcggataa cccatacggg tggcgatttc actgatgatt tgccagtccg
    14281 ttttcagatc ccatttcggt tcaactgctt tgaagaaacg ctggaagcca cggtcagccg
    14341 cagtaaacac gccttcatgc tcgccccacg acgttgacgg taaaataaca tccgccgccg
    14401 acgcggtttt ggtcataaag atgtcctgaa cgataaccag ttccagatct tcaaaggctt
    14461 tacgtactgc cgacagctcc gcgtcagttt gtagcggatc ttcgcccata atgtacgcgg
    14521 cacgcacttc gccatgcgct gcgcggtgcg gcagctcgct gatgcgataa ccggtatgag
    14581 caggcaggct ttccacgccc caggctttgg cgaatttctc gcggttagcc ggatctttta
    14641 cgtactggta tcccggatac gtatccggca gcgcgcccat atcgcaggca ccctgcacgt
    14701 tgttctgacc acgaaccggg ttaacacccg catgtggctt accgaggtta ccggtcagca
    14761 tcgcgaggct ggtcagagaa cgcacggttt ccacgccctg gtagaactgg gttacaccca
    14821 tgccccacag gatggcggcg cttttcgcct gggcatacat ccgtgccgcc tgacgaatct
    14881 cactggcgct gacgccggtg atatcttcaa ccgactccgg cgtgtagcct tcaacgattt
    14941 tacgatactc ttcaaagcct tctgtacggg aagcgacgaa tgctttgtcg tacagatttt
    15001 cttcaataat gacatggccc atcgcattca acagcgcgat gttcgagccg tttttcagtg
    15061 caatgtgcat gtcagcaatg cgcgcggttt caattttgcg cggatcgcag acgataattt
    15121 tcgccccgtt acgtttagcg ttaattacgt gattcgccac gattgggtgg gaatccgccg
    15181 ggttgtaccc gaaaacgaac actaaatcgg tattatcaat ttcgttaata gcattgctca
    15241 ttgcgccatt accgaccgat tggtgcagac ctgcaaccga tgggccgtgt cagacacgag
    15301 cgcagcagtc aacgttattg gtaccaataa cggcgcgcgc aaatttttgc attacatagt
    15361 tggtttcgtt acccgtacca cgcgaggagc cggtcgtctg gatggcatcc ggaccgtact
    15421 tctctttgat ggcgctcagg cgctcggcaa cgtaattcag tgcctcatcc caggaaacag
    15481 gttcgagttt gccgccacgc tggcgacgga tcatgggggt tttcaggcgc ggggtcagga
    15541 tctgggtatc gttaatgaag tcccagccat aataaccctt cagacacagg gtaccctggt
    15601 tggttttccc ctgcgctgcc tccgcccgga cgattttgcc gttatcgacg accaggttga
    15661 ttttgcaacc tgatgcgcaa taggggcaaa ccgtgacgac ttttttcatc ggtctcgctc
    15721 cagttaatca aatcacgcat acgcgctctc gactacagta tgcatctttt atgccacatt
    15781 ttatttgggg tcattccctg atattacggg cactatttat tcaaaactct gacgaaaaac
    15841 aggctgtcgt cagttttgac gtgacgaaac gaaataccgc gtgacagcca tcacacggca
    15901 gacattttat tttttctcga cgacgggagc cgcctgatac ccgctgccca gcgatttcat
    15961 caactgaatg ctttggatca cccggcggct gtccagcatc agtaatgaca tctcttcggc
    16021 aagcaccggc aaccgggctt cggtagcctg taagcggctg gttaagccgc gctgataggc
    16081 ggcctcggca gcgcgctggg tgaagcgcgt tgcttccacg cgttcagcct gcatttctcg
    16141 ctcgtcgttg agcgtttgca gacgcgtgcc gttgacggca acgtcacgca ccgcgttcag
    16201 tactgactgg ttgtaacgtt caatcatcat gttgctggcg gcgcgagtgc cttcgagatt
    16261 ggcattcaac cgtccaccgt caaacagcgg caatttcaga cccgggatga agttgaactg
    16321 gcgactggtt tttttgaata aggtatccag gtggatggag tccagaccga aaaacgcttt
    16381 gatatcaaag cttgggtaga acagcgcccg cgcggaatcc acctgatcta atgacgcctg
    16441 aacataccag cgcatggctt gcagatccgg gcgtctggcg agcaactcat aagagagtgt
    16501 cgccggaata ccggtctgga ctcgtggtaa tgccactggt ttgatctccg gcatatcgct
    16561 ggctcccgcg ccaatcaatg cacgcagaga ttctcgcgtt tcggtgattt gccctttaac
    16621 ggcagcaatt tgtttatcga ccgccagaat ctgtgcccgc gcgccgtgga aaggcacttg
    16681 cgcttccaga ccgtgcgcca ctttactttg gtgcgctttc accgcgtaat caatcacatc
    16741 gcgagtttgt tctaacagat cgagcatctg atagctggcc tgcatactgt aataaagctg
    16801 cgctacgccc gtggtcagcg atagctctac tgctgcggtt tctgccagcg cggcattatg
    16861 cgcgccaatg gcggcggtaa ccgctgagcg atgcacaccc cacaaatcaa gatccagtcc
    16921 ggcaaacaaa ccgactgtgg cttccgtata gtacggcccg tccataccca gtgctggcgc
    16981 atccatcgca taagggctta aaaagccgtt cgccgagacg cgttggcggt tgagcatccc
    17041 taacgctgcg acctgtaatt gtgaaccggc atctaacaaa tcggcctggg actgcgcttt
    17101 ttcttcccgc agtttcgctt cggcgagggt gtgtgaacca cttagcgtcc gttggatcag
    17161 cgcatccagc tgcgggtcat tgagttgttt ccaccactgc gcctgcggcc agccggagct
    17221 ggcaagatga atatcatcgg ccagtttgat ttgttccggt ttgagctgtt gatgaggcgc
    17281 agaatccttg cgtaccaggg cacagccgga aatcagcgtc gtgctgccga gaatgctgca
    17341 caacagcaga cgtgaaagtt gacgattgat cattgcgtgg ctccttgtgc ctgttccgtg
    17401 gcgggcgtta atgccggggc tgtccagtct ggcaagcggg cgaaaagctg gcagacgtgt
    17461 tgctcctgtt ttaataaggt gggcaggatg gcctgcgagg cgggtgtctc ttcaagcgtt
    17521 atttgaggag aacggctgag tgcggttgcc agaccggcag cgtatttttc cagggcgtcg
    17581 gcaaactgac ctgctttgtc cggctgtagt gcgttatcca gcgcctgcgc cgagttccag
    17641 gtggcatccc aggcgtgaag aagatcgcgg ccctgatgaa taaccgtttg cgaacgttca
    17701 atcagtaagg cgcgttcttc gctgtccagt tgacgctcca gcgccacgcg ttggcacatc
    17761 tcttcacagg cattaaacgc cgcatgcaga cctatacgaa tttgcagata agtgcgcaga
    17821 gccgtgactt cctgctggcg tgggatccgc attactttac ttagcatacc cagcgcgcca
    17881 gccagttttt gcggcagcgt gcgcgcttca ctttcaggcc agacaaaggt gtaaatcacc
    17941 gcagacacca cggtaccaat gatgataccc agggcgcgat cgcgaatttc caccaggtcg
    18001 tacaccggac caaaaacgtt ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg
    18061 ccgatataag aagagcgttc agagctggtg gcaatccatg cgcccaacag gaaaatcggt
    18121 gccagcacaa acagcaattc gacaatattg tccagccagg gcatgaccag tagcgtgaat
    18181 aacagcgcca gaatcgcgcc gcaaaaggcc ccgccaaaac gcagcaccat cttctggtac
    18241 gatgaaccga cattcggatt agcgacgatc acgcaggtca gcatacaggt gtgaatgcct
    18301 tcccagtcca cgccgctgta gaaggtgtaa cagatcaaac aggcgagcag cgtttttacc
    18361 gcgtagcgca tatagtctgg attggtaaaa gcatcagcga ccattgatgg cggtttggcg
    18421 gcgggcgttg gcggcgtatt cgggtccatc tgacccagtt gtaacaacgt ctggcagata
    18481 ttctccaggt tacattcccg tgccgccatc gcttcacttt cactgatccg ccagtcgctt
    18541 tgccagcact gaccttcggt aatggcatgc tgcagcttgt tgatttctga agctaatttt
    18601 tgtcggaatt caataattgc ctgagaatca gcaaaagagg tgggatcgta gcgattcagc
    18661 gtcgagtaaa tgtaggttac cgttgccacg cagctttgcc accatgcgct ttgagttcgc
    18721 cagttggcat cgtccgcgag gcaaaagaca ttgagttttt gcagcgccag cgcctctctt
    18781 tcaatccgcg tttcgggtag cggtgcgagg ctgtccgtca ggtggctaat ggcatcatca
    18841 agccgatcat taagcgcttg atgcatttgc gaaatggcac gactgggaaa ccacagtacg
    18901 ccgattaacg tcattaataa cgtcgggtag aggccaacaa cgatacacca cagcgttaag
    18961 cgcacgacca cttccggata gtcgagcatg gcggggaagg tttgcccata aatggcgaca
    19021 atggcgacag cgaaaaagac cagtcccaac cgatgggtgc gcatcaaaaa catgcagccc
    19081 atcaggatcg gcccggcgat gatcaagcgg atcaacggtt cgccgtatga ccatttgtag
    19141 atcaaaaaca ggctgccgat ctccagcacc gtggcaacca caaacaagat cgcgacaaat
    19201 ttggtgtaaa acgcgttcga ctgaataccg taaaacagca ctgccagcga taacgccaca
    19261 aaagggatct caaaagtcat cgagatcaga atcaccagca ggcagcctac ccagagttgc
    19321 acggtctgcg gcacgcgacc tggtcgccgc tcgcttaact cttcatgaaa gaacgccagc
    19381 agcctgacca ccggtaatgg cagggagttg agcgcgctca ttattgcggc tcaagattag
    19441 cgactgccga agcgccgatg cggaacattt ccgggtcagg tttatcaacc atgattttga
    19501 ccggaaaacg ctgggcaaca cggacccagt taatagaacg agaaactttc ggcaggccgc
    19561 ccagcaccag gccgccgtca tccggtagca cgccgtagcc aattgaatcc actttaccct
    19621 cgaaagtttt gccgctgtca ctcatcaggc gaatcgttgc aggtgtacct gagcgaatat
    19681 ttttcagatc ggtttcgcgg aagttggcga tcacgtacca gtgacgagtg tcgattaggg
    19741 taaaaatagg gcgcatggca gaagcaaatt gcccgacgga ggttttgagg gaaatgaccc
    19801 ggccatcaaa cggtgcgcga acggtcgcca tttccagatg cagtttggtc agggcaatat
    19861 ccgcttcgac cgccgcacgc tgggcaacta atgcatccac gccgctgacg gcgctggcgg
    19921 ctgactgcgc ctgcaacaat acagcattaa gatcggcttc tgcagcgcgc tgcgcggtcc
    19981 tcgcacggtc aacgtcttcc gctgagacaa aaccttcttt cagtaatggc tcggtgcggc
    20041 gtaatgtatc tgtggcctgt ttagcggcgg cacgggcttt ttctaccgtg gcgttaaccg
    20101 agtcggcacc aaactgttgc gcgtcaacgc tacgctgggt gagcataatt tgcttatcca
    20161 gtgccgcgag ggaggcttca gatttcgcca gattggcttc gtacgggcgc gggtcaatgc
    20221 ggaacagcaa atcgccctgt ttgactgcct ggttgtcggt gaccgccagt tctacaatgc
    20281 ggccgctgac ttccggcacc acatcaatgg tatctgctga cacgtaagcg tcattagttg
    20341 atggcgcact gtctacgcgc cagataacga aaacaagggc aaccagcgcc aacgcaacca
    20401 ctaacagagc agggaattta ctgcgaggag cttttttcgg cgtactttcc atgagcgcgt
    20461 ctcaaataga ttagaaaaat gccagccaga acagcatggc gtagagagca aaaagggcgg
    20521 tataaataat tccgacaaat gccagcttga tatttgcccg ctgaataact cgcctcgtga
    20581 tgagcgtcaa tataaggctg gcaatggcgc agaaaaacca gctgggataa taagcgccga
    20641 tcaccgggat tgccggagat aatgagcatc ccgaaaccat gaagacggga attatccagg
    20701 ctgttttttt gagttgcatc gccatgcgag agagaactgt tggcatatta aatacatcct
    20761 gtctgctgcg aacaatatct gtagtgaata tcaatatcca ttgaatgcgt cagtatggct
    20821 attttcttga tgaataaaaa tagctttatt cagccaccgc attcataact tatatatttt
    20881 atttattaat tggctgtata tatttttaaa ataaatgctt ttgggggctt cagcgccccc
    20941 aaaagcgagt gacattaatt tggggttacg atattcaccc acaaatcgaa attatccaga
    21001 caggcgataa tttcttccag tttcgcgcca ttgccaataa ttttggcttt cttcgctttc
    21061 accagatccg ccatttttgc ctgtccggtc agcacggcgt gcagatcttc acggctgatg
    21121 tagaaagagg cgtcggcttg cggttgcagt gttttacggt agttaagcac gctatcgttc
    21181 agcgtcaggt tgaggttatc gccgttgctc atattgaagt tcaggctgat atttttaccc
    21241 gcagctttcg cgctatcgag gcgaacggcc ataaagtcga acagcatttc gaccgacatt
    21301 ccgcgaatgg tgtccgggga accggtggtg ccgtggctga acttatgcac cccttcgcgc
    21361 agctctttcg cgccggtcag gtagaaaccg cgccatgtgg cggactcggc ctgatagccc
    21421 agctgttcaa agttattcgc ttgcagattc ttcgccacct ggtcacccgg gttggcggca
    21481 atcacctgtt tcagtagttc tgccgaccag cggtaatcac cttgcttatt cgcttcttgc
    21541 gccaggttga tgatacgggc agagccgccc agcgctttca cgtaacgttt gcccatctct
    21601 acctgaccat acggatgcag gttagccggg ttaccgtcgt aatagccaag atagaagtta
    21661 tacaccgcgc gggcgttgtg gctgacagaa ccgtaatagc cacggctggc ccagttattg
    21721 gcaagtgcag gcggcagctt aatcatgtcg ccgatttcat tcatggtgta gccctggttc
    21781 gccaggtgca gggtctggtc gtgaatgaac gtgatagtat cgcgatattt accaatataa
    21841 tcattgatat gcttattgcc ccagaccggc catgtgtgcg gcataaacag tacttccgcg
    21901 tcgttacccc acatatccag cgtttcgttc aggtactcgg tccacttgct ggtatcgcgg
    21961 gttttcgcgc cgcgcagagt gtagaagttg tgcagggtat gcgtggcgtt ctcggcggta
    22021 cacagggctt tcagggccgg aatatagaag tgcatttcgg ctggcgcttc gctacctggg
    22081 gtcatcagga agtcaaactc caggccgtcg ataatcatct tctcgccagt tctgacgata
    22141 gttttagtcg gtgcaataat gctcgggtcg cccgttgcca gcgtcacgcc aaggccatta
    22201 cccacgttgc cttgcgcgtt gtgcggcagt aacagaccgt aagagtagag cgcacggcgg
    22261 ctcatgatgt tacccgccag cacgttttcg ctgatggctt cgtccataaa gccagcaggg
    22321 gcaatcacct gaactttgcc ggatttaaca tcggcttcag agataatgcc tttcacgcca
    22381 ccatagtggt cggtgtggct gtgagtgtag ataacggcaa caatcggttt ttgcggacga
    22441 tgctggaagt aaaggtcaag tgcggctttt gcggcaggcg gcgtcaccag cgggtcgatg
    22501 acaataatgc ctttctcccc ctcaacgaac gtaatgttag agatatcctg gccgcgcacc
    22561 tgatacattt tatcggtgac tttgaacagg ccagaaatgc cgttgatctg cgactgacgc
    22621 cacaggctgg ggtttacggt ttccggcgca gcggcattaa tatcaaattt gtaatcgtct
    22681 gcgcggtagt aaactttacc gttcgcatca cgcagaatac cttcatccag cagcggggcg
    22741 ataaatccac gctgggcatc gtcaaaatcc tgacgatcgc taaacggtaa tgatttggcg
    22801 taattctgat taacctgttg ggtatattga gtggcggctt tcgcttcctc tttcgcgtta
    22861 gcggtattta ccatcatgcc gctggcggca gttaacgcaa taacaatcct gctcaaacgg
    22921 aataaccgag agttattcat ataaatctcc atttatcatt tatgtcgtaa atatgtaatc
    22981 catgcaaaat acgtcattta aagctggcgt tatcataatt gggaaattgc gactttaata
    23041 agtggaagtg tgagcggaac gatccatttt attaggcatt tgtgctgaag agggaaaagt
    23101 acgacatatt tattactatc atcaataatg tgacctgact tatattttta ttatttatat
    23161 tactttcagc tttcacttcg acaagaagta cgcacaatga ttaatcagat gaatgatgcg
    23221 gagtgtaacc atgaaacccg tcgtgctggt ggtggatgat gacacggcta tctgcgcact
    23281 gttgcaggac gtgttaagtg aacatgtctt tacggtttca gtctgccata ccggacagga
    23341 ggccatattg cgtattgagg gcgatcctga cattgcgctg gtggtgctgg atatgatgct
    23401 acccgatacc aacggcctga gggtattgca gcaaatccag aaactgcgtc cgacattgcc
    23461 ggtggtgatg ttgaccggca tgggcagtga gtctgatgtg gtggtcgggc tggaaatggg
    23521 cgcggatgat tacatctgca aaccgttcac cccgcgggtg gtggtcgcac gactgaaagc
    23581 ggtgttgcga cgggttggcg cgctggcggt taatgacgag aaaagcgccg ggctgtcatt
    23641 caatggctgg tatctcgata ccatgcgctg ccagttgcac aatccgcttc agcaacatgt
    23701 cgagttaact cagggcgaat atggcttgtt actggctctg gcgcaaaacg cgcgtcgggt
    23761 attgagccgc gagcagttgc tggcgttcac ccacagcgac agcgtggagg tgtttgatcg
    23821 caccatcgac gtattaatca tgcgcttacg acgcaaaatc gaactcaacc cgcatcagcc
    23881 gatgttgatt aaaactctgc gcggtctggg gtatgtcttc gctgccgacg tgcattaatc
    23941 cgacatttct gtttgcagct cttccagcgt tttcgccccg tcgatggcat tggcgcataa
    24001 caggcttgcc cgcgcgcagg cgtgtgccag tttgatgctc tcggtaagcg tccagcgttc
    24061 atggcagcca tataaaaatc cggcacaaaa tgcatcgccc gcgccgacgc tgccgataat
    24121 ttcccgttgt tctaactttt gcgaagggat ccacacgccg ccctgttctg gggtctctcc
    24181 ccacgcccct tccgggtagt ggatcaccac ccgctggcgc acgcccgcat ctaacagttc
    24241 acgcgcggcg gcagcaatat gcgcgatatg cggttcgccg ttgggtaacc gaatttccag
    24301 cccgctaaac tccccggcct ccagttcgtt aattaccaga taatccagat ggcgcaacgc
    24361 gggcagcacc agcggctgat aacgcggatc gcccttacgg gagaccaggt cgagtgaggt
    24421 ttcaaagcca gtatcacgca tttgcgccag caggcgtgcg ctacgggtgc cgtaaacatc
    24481 gtccggcata tccaggctgt cgagcaacag taaatagccg agatggaaaa tcttcatggt
    24541 gttatcgagc tgatcgaacg ccggaagatc caaaagtctg tttgcacccg gcgaatggaa
    24601 aaacgtccgc tgcccgcttg ggtctgtcat cacctgcgac atcgacgtcg gggcaaaggt
    24661 agtgcgctgc acatgctgac gattgacgtg atattgctca agcatcgcca tgatgtaatc
    24721 gccgtcgctg tcctgaccga ttagcccaac cgcctgtaac ggcagcccgg tgcgcatttt
    24781 cgccagcgtc agcaggacgt taagcggcgc accgccggtg gagcgttcgc tgtgggtgat
    24841 ctccgccagc cagccgcgct ctggccactg gataatctga tggacgtggt caaccaacat
    24901 attgcctgcg gcgataatcc ctttacgctc catcatgcct gccccgcact gccaaaaata
    24961 cgcatctgtt gtgcaaccgt atcgctgatg gcggcttcca ctgccagcag taactcggca
    25021 aactcgtcat acatcggctg acggtgcgcc attcgggcct caacggcagc cagcgccgct
    25081 tgcgacatgc cagtgtagaa attgattttg tggataccca gttcgatggc gcggcggaaa
    25141 tcggcatcgc taatccctga gccgccgtgt aataccagcg ggatagcagc ctgctggcgg
    25201 atagcgtcga ggcgcgggaa atcgagtttc ggttcgccgc gatatttgcc gtgtgcattg
    25261 ccaatcgcca cggccagcgc gtcaatgccg gtacggtcga caaaatcacg cgccagttgc
    25321 ggatcggtaa acagggattc atcggcatga ccatagagtg caccgccttc gtcgccaccc
    25381 accgcgccca gttcagcttc taccgatacg ccaacggcat ggcacatttt caccacttcc
    25441 cgcgtctggc ggacattttc ttcatacccc agcgtggagc catcaaacat taccgagctg
    25501 aatcccagcc gcaacgcttg caccaccgat tcaaaatgca gtccgtgatc gagatttaac
    25561 accaccggaa tgtcatgacg cgccgcttca aatttcactg tctcgaccag ggaatccagc
    25621 gagatatatt taaaatgtac ttcggcaatg ttgataataa agggcgaacg ttcttgtctg
    25681 gcagcagcaa acaaggcgcg taaaaagtgc gagtccagca cattaaacgc acctaacgaa
    25741 tagtgatgtt cgcgggcatg tgccagccct tcagcgagag aaatcaatgg cataacgtta
    25801 tccttattca gcggaactgg cggtattcgt tacacaatac caactgtgca ggttcgtctt
    25861 cgtcaatcag gttgtagcgg tccagtggct gtaaaaagtg gttatcgtgg tcgtcgtcat
    25921 taacagaaga gacttcgcca accagcacat cgccaaaacc ggcttctgcc caaaagctgt
    25981 gatacaggcc gggcggcagg cagatacttt cgccgggaga caggcgcaac tgagagcctg
    26041 cagtatgttt ctggcggcag ccgtctatca ccaccgtaat gtcactatca gccgtttgct
    26101 cgttactatc ggcattccac agttcaacaa ttaaattacc gccgccgcga ttgataatgt
    26161 cctcacgctt acgccagtga aaatgcattg gcgtgacctg cgcatcgcgc acatgcatga
    26221 tcttctcggc gtaacatttc acatacggca ttccttttgc tgagccgtta cgtagggtaa
    26281 acagcgtcag cccgtgtgct gcaaagttgt tgcgaccaaa agcggtaaca tcccagccga
    26341 gtttaagatc aaaaacctcc tcccacgcgg cggtgtcgag ctgctgccat tgagcaggcg
    26401 agaaactggc aaagggcggc agatgtacgt catgttgcga aaagaattgc cgtgtatggc
    26461 cgagaatgtc gtttatagcg gagcgtttca tcgaaagcgt tctccttagc ggtgaccggg
    26521 cagctgcgcc gcccggcgtg tggacttatt tcaccgtcca gcctttgtaa ttgccgacgt
    26581 tgtttttatc gatcatcgtc accgggatca gcaccggctc tttcggcgca ggtttgccct
    26641 gcaaaatgtc gtagccaatt tccaccgctt tcgctgccat cacctgggga tcttgcgccg
    26701 gagtagcgac aaacaacgag ttctcacgct tgagcgcctc ttcggcgtcc gggcttccgt
    26761 ccacaccgac aataaagaac tcgttacgtt gtgcctgttt tgctgccaga tcggcaccga
    26821 ttgcggtggg atcgttaatc gcaaatacgc cgtcgatctt cggatttgcc gccagcagtc
    26881 ccgtcattac ctccagcccg ccttcacgac tgcctttcgc gttctggttg tcagagagca
    26941 ctttgatgtc aggatggcgt ttaaattcag tctggcatcc ttcgacgcgg ttttgcacgg
    27001 cggataccgg cgggccgttg atgatcacca cgttgccttt gcctttcagg cgatcggtga
    27061 tgtatttaca ggccatttcg cctgcctggg tgttatcgga agtgatggtg gcatcggcac
    27121 cttctgccgc tacgtcaacc gcgacgacca cgatcccggc atctttcgcc cgtttcaccg
    27181 ccgggccgat cccttttgaa tcggcagcgt tgaggataat catatcgacg ttagcggcga
    27241 taaagttgtc tatctgcgac acctgctgac ccagatcgta gccgctggaa accagcgtca
    27301 ctttgacgtt atcgcctgcc agtttgcgtg cttccagctc cgcgcctttg gtgatctgca
    27361 cgaagaacgg gttcgccaga tcgcccaccg ttacgcctat tgatttcagt tctttcgcct
    27421 gggcgaacgg tgctgcggcc agcagcgcgc ctgcacagag cgcggtcact aagggcttca
    27481 atctcatgct gtattcctca tgtgtttatg cactttgatg gtgtcgggta cggtatttgt
    27541 cgatcagcac cgcaatgatg atcatcgccc ctttgatcac cagttgccag aagtaagaga
    27601 cgcccattaa ggtcatgcca ttgttgagcg tggcgataat tagcgcgccg accagcgtgc
    27661 cggtgatggt gccgatgccg ccgacaaaac tggtgccgcc gaggatcacc gctgcaatgg
    27721 catccagttc gtaacccatg cctaagttgc cgttggcgct gtacagccgt gaggcgctca
    27781 taatcccacc aagcccagaa agcaggccgc tcatgccata aacaaacagc agcaccagcc
    27841 agaccttaat gccggtaaga cgcgccgcct gcatattgcc gccgacggcg tagatatgaa
    27901 cgccaagcgt ggtgcggcgc agaataaacc agcacaccac gatcaccgcc agcgcaatca
    27961 ccaccagcca tgggaccggc ccgagatagt tgttgcctat ccactcgaaa ttaatgttgg
    28021 agttgataac cgtggtgccg tccgccagca gatacgccgc cccgcgcagc gccgtatagg
    28081 tgccaagcgt aacgataaac ggtggcaatc cggcaaaggc caccagcgcg ccgttgaaca
    28141 gccccagcag caggccgagc atcagcgctg ccgggatcga cagcatggcg aactcagggc
    28201 taagggagac gaccatcgcc gccaccgctg tggtgccgag gatcgaaccc acggaaagat
    28261 cgatcccgcc ggtcaaaatg atgaaggtca tccccgccgc cagaacaatg ttgatcgatg
    28321 cctggcgggt aatattcagc aggttgcttt cggtaaaaaa gtttggcgtg ataaagccaa
    28381 ataccgccac gatcagaatc aaaatgggca agatgccgac cgtttgcatc agatcgctca
    28441 tcagcatttt tttggcagag gtttttgcag aggtcgtcat gaggttaccg cctgggaatg
    28501 ggcgtcgttg acgccagtcg ctaaggtcat aatgttttcc tgggtgatgt ttttgccgtt
    28561 taactcaccg gcaatgctgc cttcgcgcat cacatagacg cgatcgctca ttcccactat
    28621 ctccggcagt tcgctggaga tcatcaggat cgccacgccc ttgcgcgcca tctcgttcat
    28681 gatccggtag atctcacttt tggcgccaac gtccacgccg cgggcgggtt catcgagcag
    28741 taaaatgcgc gggccaatcg ccacccagcg ggagatcaac agtttttgct gattgccgcc
    28801 ggaaagcccg cccgcgcgta cctgggcatg aggcacgcga atgttgagca acttaatggc
    28861 gtcatcggaa atggtttgcg cttttttgcg attgagcatt ccccagcggg catcgcgctc
    28921 cagggttgcc atggtgatgt tttcggctgc tgccatttca aggaataagc cttgttcttt
    28981 gcggttttcg gtgagaaaac caatgccaag atcgatggct tcgcgcgggg agtggatcac
    29041 caccggttca ccatcgactt caatcattcc gcctgtcgct ttccgcacgc cgaagatcaa
    29101 ctgcgccagt tcggaacgtc cggcacccac cagtccggcg aggccaacaa tttcgccgga
    29161 acgcaccagc aggctactcg gctggacttt gccgccgtcg atcaggtggt gaacattcag
    29221 gcgggcttta ccgagcggga tatcgcgctc tttattgaac agatcgctca gtggcctgcc
    29281 gaccatcatc cgcaccagct ccccggcatt gaggttatcg cgggtcaggc tgccaacgta
    29341 ttgcccgtcg cgtagcacgc tgacgcgatc ggaaagctca tacacttccg ccatgcggtg
    29401 gctaatgtag ataatcgcca tcccttcatc gcgcagccgc ataatcagtt caaacaggcg
    29461 gtgagtttcg cgggaggaga gggcagcggt gggttcgtcc atcaccagaa tgcggctgtt
    29521 gcgatgtagt gcgcgggcga tttcgacctg ctgttgctcg gcaatggtca gcgtcatgac
    29581 cttatcgctg gcgttaaact gcgcgccgag gcggtcgatc actttttgcg cctgcactag
    29641 catctcttta cgctgcacca gcccgccgtg ggaaagctcg ctgccgagaa aaatattttc
    29701 tgccaccgac agattcggtg ccagctgcat ctcctgataa atcagcgtaa taccggcgct
    29761 taaggcatct tttggcgtgc gaatgtgaaa gggcttgccg tctatgagaa tctcgccgct
    29821 ggtggcggta tacgcccccg ccagcacctt catcagcgtg ctttttcccg cgccgttttc
    29881 ccccatcagg gcatggatct cgccagggta gaccgtcaaa tccacccctt tgagtgcata
    29941 aaatttgccg aaggctttgg caatattgcg catctccaga actggcgtgc tgttcatggt
    30001 ggactcctgc gaggttgtcg atatcagcac tccatcacag gaaagtaaat gcaaaatgtc
    30061 agaagccgtt catatttgtc atattgcgat ttttcttcac ggagcgtgat atggcgcacc
    30121 ccggacgtca cttttttgcc agcgcgcgcg ggcgattgct gcttttgaat ttgctggtgg
    30181 tggcggtgac gttgatggtc agcggcgtgg cggtaatggg ctttcgtcat gccagccaga
    30241 tgcaggagct ggtgcagcag caaacggtgg atgatatgac cggcagcctg aatctggcgc
    30301 gcgacacggc aaacgtggca acggcggcgg tgcgattgtc gcaggtggtg ggagcgctgg
    30361 aatataaagg cgaagccgag cggttgcagg agacacagcg ggcattaaaa agctcgcttg
    30421 cacaactggc gaacgcgcct ttagcgcagc aggaagccgg gctggtgacg cgaatcatca
    30481 cgcgtagcaa cgaactgcaa accagcgtcg gcggtatgct ggagcgcggg caaaggcggc
    30541 atctggagcg caacgcgctg ttaagttcgc tgtatcaaaa cttaagttat ctgcggcatc
    30601 tgcaaaaagt cactcacgcg caagatgata ttttgcttaa cgagatgaac cgactgattg
    30661 ttgccgctat tgccaccccc gcgccgcagg cgattattca tcaactggtc ggggtaatgt
    30721 ctgcgttgcc gacccacagc gatacgccat tggttaatac gttactcaat gatttcaatg
    30781 atttcaatga tttcaatgat gaaatgcgta aactcgcgcc gctttctgcc gctctggagc
    30841 aaagcgatct ggcaatcagc tggtatatgt ttcacatcaa agcgctggtg gcgatcctca
    30901 atggcgacat caaccgttac gccgaacagg tggcgacgct ctccggtcag cgtgtggcgc
    30961 aaagttacca ggatttgcgc tccggcgaga gggtgatcat gattttcgcc ctgttagcgg
    31021 tggtgatcac cgcgtttgcc ggctggtata tctatcgcaa cctcggcact aacctgacgg
    31081 cgatttcccg cgccatgagc cgcctggcgc acggcgaagc ggacgtcact tttccggcgt
    31141 tacagcggcg tgatgagttg ggtgaactgg cgcgcgcctt taacgttttt gcccgcaata
    31201 ccgcgtcact cgaacgaacc tcacggctgc taaaagagaa gaccacgcag atggagattg
    31261 cccaaactga gcggcagggg ttggaagagg cgctattaca cagccagaaa ctgaaagctg
    31321 tcgggcaact gacgggcggg ctggcccatg attttaataa tctgctggcg gtgattattg
    31381 gcagcctgga gctggtgaat ccggactcgc cagacgcgcc gcgaatacag cgggcgctgc
    31441 aagctgccga acgtggggcg ctactgacac aacgactgct ggcgttttcc cgcaagcagt
    31501 ccttacatcc gcaagccgtt gaactgaaaa cattgctgga agagttaagc gaattaatgc
    31561 gccactcctt accggcaacg ctggcgctgg agattgaagc gcagtctccg gcgtggcccg
    31621 cgtggattga catcagccaa ctggaaaatg ccatcatcaa cctggtgatg aacgcccgtg
    31681 acgcgatgga agggcagacg ggaatcatca aaattcgtac ctggaatcag cgcgttactc
    31741 gcagcagtgg gcagcgtcag gacatggtgg cactggaggt catcgatcat ggctgcggta
    31801 tgtcgcaggc ggtgaaaagc caggtatttg agccattctt taccaccaaa cagacgggca
    31861 gcggcagcgg gctgggtttg tcgatggtct atggttttgt gcgccagtcc ggtgggcgcg
    31921 ttgaaattga aagcgcgccg gggcagggga caacggtcag attacagctt ccccgcgcgc
    31981 tgacggcggc gaaaaaccta tcgccagcgg cagtagaaca ggcttctgtc agcggcgata
    32041 aactggtgtt agtgctggaa gatgaagccg cggtgcgcca gaccatctgc gaacagttgc
    32101 acctgctggg ctatttgacg ctggaagcaa gcagcggcga acaggcgctg gatctgctgg
    32161 cggcatcagc agaaatcgat atttttatca gcgatttaat gctccccggc ggcatgagcg
    32221 gcgcagaagt ggtcaatgcg gcccgcaaac tttacccgca cctgacgctg ctactcatca
    32281 gcggtcagga tttacgcccc agccataacc ccgcgctgcc agacgtggcg ctgttgcgca
    32341 aaccctttac ccgtgcgcag ttggcgcagg cgctggggca agagaattga gaatcctcct
    32401 ctactcgtaa caggcacctt tgcctacctt gtattaactt tgttgcgagg ccgagtttat
    32461 gaaacgccac tccacgcttt ttcttttcac cctgctcacg ctgaccaccg taccggccca
    32521 ggccgatatt atcgatgaca ccattggcaa tattcagcag gcgattaacg acgcttctaa
    32581 ccctgaccgt gggcgagatt atgaagattc gcgcgacgac ggctggcagc gtgaagtgag
    32641 cgacgatcgg cgcagacaat atgacgaccg ccgtcgccag tttgaagatc gtcgcaggca
    32701 gctggacgat cgccagcacc agcttaatca ggagcgtcgg cagctagaag atgaagagcg
    32761 gagaatggaa gatgagtatg ggcaatgatg ttgggtatgg ggcaggcatc gcccctctgg
    32821 ggagagggtt agggtgaggg ggccagcacg cacattccct ctcatcacgc caccccaatc
    32881 tccatcccat caaaccccgc ctcaaacccc gacggcagtg cgttttccat cagccacgca
    32941 tcaaactggt ggctgatatg ggtcagaacc acgcgcggcg agcggataac ctgattcagc
    33001 gcaagcacgg tatttaaatc acagtgatta cgcggtgcat cctcgcgcgg cgggtggctg
    33061 caatcaatca ccattacctg cggctgatta ttgcgtaaaa atttcagcgt tttttccggc
    33121 aagccagcgg tgtcagacag ccacgccacc cggctgtgcg ccgtttccag cagataaccg
    33181 aaggtcagtt ttgagtggtt gagcggcagg ggtgtgacct gtaacccctg caaatcaaac
    33241 accacaaacg gttccaccgt gtggctgaaa tcaagcaggc ccggatgttt aaacagatcg
    33301 tcgcagccct gttcatccgg tgggccgtaa accgggatcg gatcgcccac gccccagcgc
    33361 agcgggaata gcccctgcac gtgatccata tggtagtgcg tcagcaaaaa ctgctggaac
    33421 gatccgggcg accagcgatc ggcgagatcg tgccgcccgg cgtcgatcag ggtgatcgcc
    33481 tcgttaaact tcactacgcc gctgcatggc tggcggcgat agtgcggcga gcgccgcgct
    33541 ctggcgcagg ccgcacactc gcagccccat gccggaacgc cctgtgcgcc gccggagccg
    33601 gtgagcgtga gggtcaggct catgttacag caccttggtg aagcggaagt ggctctgctc
    33661 gtagccttcg cgcagataga aacggtgcgc gtcgtggcgc ttcacgttgg tcgaaagttc
    33721 ggtcatttcg gccccggcct ggcgggcttc ttcttctgcc cacgccagta acttactgcc
    33781 gacgttcaga ccgcgcgcct gcggcattac caccaactcc tgaatttcgc cgatccagtt
    33841 gacatgatgc agatgaaact gcaaatgcag gccgatcata ccgacaactt cgccatccag
    33901 cagcgccaga tggtagcgca tgttcgggtc gcgcaggttg gcgttaaagc ccacgcgaaa
    33961 cgcgtggtgg tcaaactccg cctgttttag ctcgcaaatc aacgcgtaaa ccgcgtcggt
    34021 gtcgtactgc gtggccgggc gaaagctcac aagcaggcat ggtgtttctc cttctgatgg
    34081 attagcgtca gcagcgtgtc gaccgactgg cgcaggctgc cgtcattatt gagcgtatgg
    34141 caatcctgtg gagtatagcg ggcggcgcgc gccagccggg cgttaatttc actggcgttt
    34201 tcgcggccac ggttttccag gcgctggcgc aggatctctg gcgaaacctg taagcaaatg
    34261 ggcagcagcg ccgattgata gcgcgcccgc gcctgcggca gatgggcgcg tgagccgttg
    34321 accagcacgt cgaatccagc gtgcagccag aggtcaatct cgacgccgac gccataatac
    34381 agaccgttag cgtgccagct taaggccagc agattttgcc ccgcgcgggt aaaaaattcc
    34441 tgctcgctca gggcgatatg gttttcgctt ccggcgctgg cgtcgcgcgt gatgtagcga
    34501 tgcgccacca gtagctgagt ttgttcccgc aggcggagtt ccgccagcag gctatctttc
    34561 ccggagccgg acggccccat taaccaaatc agttttccca tcatcagaac acccttttac
    34621 cctgacgcca gacgtggtcg atatgaatgt gattgccctt gcgatgcgcc agcaccaggt
    34681 cggcgcgttt gccctcgcca atcaccccgc gatcctggag attaagcgcc tgcgccggat
    34741 ttttggtcac cagcttcacc gcctgcggca gcgtaaagcg gttgctctgg tcatcggcga
    34801 cgcggaacgc cgcatccagc aggctggcgg ggtagtagtc ggaagagagg atatccagca
    34861 ggccaagctg tgccagttca ctggccgcca cgttgccgga gtgcgaaccg ccacgcacga
    34921 tatttggcgc gcccatcagc acgttcatgc catgcttgcg cgaggcttcc gctgcttcaa
    34981 acgtggtggg aaattcggcg atcacgctgc caagctggtg agattcggca acgtgggcgt
    35041 gcgttgcgtc atcgtggctg gcgagcgcaa tttgtcgcgc gcggcacatt gcggcgatgg
    35101 attcgcgatt cggctgcgac cagcgtgcgg caagcgccag ctgttcctct tcatactgct
    35161 gcatctgcgc atcggtgagc gagtatttgc cctgataata ttcgcgatac ttctcgcggt
    35221 tggcgaactg gcgctggccc ggcgagtgat ccatcagcga caccagcgtc accggctcgc
    35281 gctgcaccag cttttcaaac agcggcagcg tggtgttatg cggcagctcg cagcgcaggt
    35341 gcaggcggtg ctcggcgcgg ttgacgccgc gtttctgcgt ctcttcgatg gcgttgatca
    35401 tcttctccag attctccagc cgatcgccgc cgtctcgcac gtcgccaatc gccacggcgt
    35461 ccagtacggt ggtgatgccg ctcgccacca tcagcgcgtc atggctgctc atcgccgagt
    35521 gggcgggcca gtcaactttc gggcgcgggg tgaagaattt atcgaggttg tctgtatgca
    35581 gctcaatcag ccccggcagc agccagccgc cttcgccgtc catcgcctcc ggcaggcggc
    35641 tctggctttc ggcaaaggcg cggatttcgc cgtcctgcat ctccagcgaa ccgtttacca
    35701 cttcgttttc cagcaccagt ttgacgttat tgataatcat gaagaggctc ccattgggtg
    35761 caggcggtcg gcgacgtcat tacgtacggc ttcgtcatgg aaaatgccga cgatggctgc
    35821 gccgcgggct ttggcttctc ggatcagttc gactacggcg gcgctgtttt tggcgtccag
    35881 cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg acgataaagc cacgggcgat
    35941 gttgacgcgc tgctgttcgc cgccggagaa ggtcgatggc gcaaggcgcc acaggcgttc
    36001 cggcacgttc agacgggtga gcagacgcgc ggctttagcg gcgcaggctt cacgcggtac
    36061 gccggtatcg agcagcggct gcattactac ttccagcgcc gaaatgcgcg ggataacgcg
    36121 cagaaactgg ctcacccagc caacggtggt tttgcggatt tccaccactt tgcgcgctgg
    36181 cgcagtgacc aggtctaccc actcgtcacc gtgtttgatc tggatttgcc cttcatcggg
    36241 cagatagttg gcgtacagcg agcgcagcag ggttgatttg ccgctgccgg agtggccgtg
    36301 gagcaccacg cattcgcccg cgttgacggt gagcgaggcg cggttgagga cgggcaagcg
    36361 cacgccgttt tgctggtgca ggatgaaggt tttactgacg ttttgtacgt taatcatttt
    36421 tcgcctcgtg gttcgggtgt cggatgcgac gctgatgcgt cttatccgac ctacggggag
    36481 cgcatttgta ggccggataa ggcgtttacg ccgcatccgg caccggctca attctgcaaa
    36541 accgatgaca ccagcagctg tgtatacgga tgatgcgggt cgtcgagcac gcggtcggtt
    36601 aacccactct ccaccacttg cccctgcttc atcaccagca aacggtccgc cagcaggcgg
    36661 gcgacgccta aatcgtgggt gacaatcact accgcgaggt tcagctccac caccaggccg
    36721 cgcagcaggt cgagcaggcg ggcctgcacc gacacatcca gcccgccggt cggttcatcc
    36781 ataaacacca gcttcggatg cgtcaccagg ttgcgggcaa tctgcaaacg ctgctgcata
    36841 ccgccggaaa aggtggtcgg caggtcgtcg atccggttgg cgggaatttc cacctcttcc
    36901 agccacttct gcgcggtggc gcgaatatcg ccgtaatgac gcgccccggt cgccatcagc
    36961 cgctcgccga tattgccgcc tgccgagacc tggcggcgca ggccgtcgag cggatgctga
    37021 tgcactacgc cccattcggt gcgcagcagg cgacggcggt cggcctcgct catgccatag
    37081 agggaacggt tttcatagtg aatctccccc tgctgcggcg tcagacgcgc ggaaattgat
    37141 ttcagcagcg tggtcttccc ggagccggat tccccgacaa tgcccagcac ttcccccggc
    37201 cataaatcaa aagagacatc gctaaagcct ttgcccggcg cgtaaaggtg ggtcaggtta
    37261 ttgaccgaaa gtaacggttg attcattggc tttttgcctc gctctgttgg cggcaataat
    37321 cggtatcgga gcagacaaac atgcggtttc cggcgtcatc cagcaccact tcatcaagat
    37381 agctgtgggt cgatccgcag atggcgcatg gctcatccca ctgctgaacg gtgaacgggt
    37441 gatcgtcgaa atcgaggctt tccacgcggg taaacggcgg caccgcatag atgcgcttct
    37501 cacgcccggc gccgaagagt tgcagggcgg gcatcatgtc cattttcggg ttatcgaatt
    37561 tcgggatcgg cgacgggtcc attacgtagc gcccgttcac cttcaccgga taggcgtagg
    37621 tggtggcgat atgaccgaag cgggcgatat cttcatacag cttcacctgc atcacgccgt
    37681 attcctccag cgcgtgcatg gtgcgggttt ccgtttcgcg cggctcgata aagcgcagcg
    37741 gttcggggat cggcacctgg aaaataatga tctgatcttc ggtcagcggc gtttcgggga
    37801 tgcggtgacg cgtctggatg accgttgcat cgtccgtgcg ctcggtggtg tttaccccgg
    37861 tcacgcgctt aaagaagttg cgaatcgaca cggcgttggt ggtgtcatcc gcgccctggt
    37921 caatcacctt cagcacgtcg ctttcgccaa tcacgctggc ggtaagctga atgccgccgg
    37981 ttccccagcc gtacggcatt ggcatctcgc gcccgccaaa cggcacctga taaccgggga
    38041 tcgccaccgc ttttaagatg gcgcggcgga tcatgcgttt ggtctgctcg tcgaggtagg
    38101 caaagttgta gccgctcaga ttagccatgg ttctgctcct gttgcagacg tttgagtagc
    38161 tccagttcgg cctggaaatc gacgtagtgc gggagtttga ggtgcgagac aaagcctgcg
    38221 gcttcgacgt tgtcggcatg tgccagcacg aactcttcat cctgcgccgg gcctgtcgcg
    38281 tgctcgccgt actccggggc ttgcagagcg cggtcgacca gcgccatcgc catcgctttg
    38341 cgctcgctca tgccgaacac cagcccgtag ccgcgcgtga agtgcggaga ttcatccggt
    38401 gggtcgataa aaccgttgac catttcacac tcagtcatca gcagttcgcc gacgttcacc
    38461 gcaaatccca gctcttcggg cacaatcgac acgtcgatgt aaccactgcg gatttcaccc
    38521 gcgaacgggt gattgcgccc gtaaccgcgt tgggtggagt aggccagcgc cagcaaatag
    38581 ccttcgtcgc cgcgcatgag ctgttgcagg cgggaagaac gtgagcaggg gtaaaccggt
    38641 ggcgtgcggg tgatgtcatc cggctgtgcg ccgctatcct cttcaaactt cgccagcccc
    38701 tgacgcgcca gcaggctgaa aacgtgcggc gacggctgct gttcgctgtc ggcggtggtc
    38761 agcgtcggcg cttcgccgtt tgccagcagg gtgaaatcga gcaggcgatg ggtgtagtcg
    38821 taggttgggc caagcagctg gccgccggga atatctttat aaaccgctga aatacgccgt
    38881 tcgagacgca tctccgtggt gtcgagcggc tcgcttaccg cgagcttcgc cagcgtggtg
    38941 cggtaggcgc gcagcaggaa aattgcttcg acgttatcgc cgctggcctg tttcagcgcc
    39001 agcgccgcca gctcgcggtc ggcaatgccg ccttcggtca tcacgcgatc caccgcaaga
    39061 ttaagctgct gttcaatctg ggcgacgctc agttcaggca aatcggtatc gccccggcgt
    39121 cggctctctt gcagggcgtg ggcggcgtcg atcgccttct cgcccccttt cacggcaacg
    39181 tacatcagca cacctccaca tgagtggttc gcggaacagc cagcaggcgc tcgccgcagg
    39241 tcaggatcag gtcgatgccg agcgggaacg ggtgcgggcg ctcggtgagt tcgtgcagaa
    39301 tgcacttcgg cagctgcggg gcgatcattc gttcttcggc aatacccgcg ccggtgagac
    39361 gcagcatgcg cccgccgctc aggctggcga cctgtaaaat cagcgttgcg cccgcttccg
    39421 gcgcaacggc ggtgccggtg gaaagggcgt taagctgttc gctggaaatc gcctcatccg
    39481 ttaccgcgaa ggtcgcctgt tccggctggc tgaccagcgg cgcgttggta tgaaaacgca
    39541 ggctctggct gacgatatcg ttgctcaatg gcgcggcaag ccacaccggc gtgtcgttat
    39601 cggccagcgt cagcagcacg ctggtggtgg cgatattcag cggctgccag ccgcgtttga
    39661 gctgatgcag ggcgacaatc acgcccggct cgctcatggc ctttaacagg cgacgaaaac
    39721 tgtgctgggc atcctgcacg ggaagcataa aagcggtttc cagggtcatg cgttgtctcc
    39781 gcgaaccatc gtaaagaagt cgacccggct ggcgttcact tcggcctggc gtgcggcaat
    39841 gcgtgccata cggtcagcgt ccagcggggc aataagcgtt tctgataagt tttgaaaatg
    39901 gcgggtttgc tgcatcagcg cgtcaatcag cgcgcagcgt tcggcgtgct gtttgtcgcg
    39961 ccccagcacc cagctgtagc cgagggtgcc gtcagtcaga cgtacggcgg cgcgggtcag
    40021 cgtggcgtcg ccggcaaaaa agcgttcgcc ggtgccgccc atccgcgcct gaatctgtac
    40081 caggccagtt tcagcggcgc ggatcacctc atagttggcg gtgatgttca gcgcgttcag
    40141 gcgggctgcc agttctgccg gttggctgtg tgccagtacg gacatccagt gctggcgggt
    40201 cgcggtatct gcgtgcattc agtgctccat agtgaattca atcatgtcgg cgcgcgtcag
    40261 gctgacggag tactccgccg ggctgctttc accgtcacgg tggttaaggg tgcgcacgca
    40321 cagcagcggg gacatattcg gaatttcaag acgctggcac tctttggcct gggcgcggcg
    40381 ggcgctgatc cgcgtctggc tgcggcgcag tgcaatcccc gtttgctcgc gcagaaaatc
    40441 gtgcagcgag ccgctgtcga agcgttgcag cgtcggccag agggtgaggt ccgcgaagta
    40501 gtggtcgatt aaacagagcg cgatgccgtt gacccggcgc agggtgcgca gatggatgac
    40561 gttctccccc tcggttatcc ccagcgcgtc agcgacgtga ccggaggccg ggcgcagcac
    40621 agacagcagt ttttcgctgg tgggatggct gccctgatcc agcagattct ggctaaaacg
    40681 cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc accagcacgc cgacgccctg
    40741 acggcgctgt acccagcctt tttccaccag ttggtcgatg gcgcggcgca gggtgtggcg
    40801 atttacctca aagcgcgccg ccagctgctg ctcggcggga agatagtcgc cgcagcggta
    40861 gtgttgacga agctcctgct caagttttgc ggctatctcc tgatagcgtg ttgggtagct
    40921 ggtcggatgt gtagacaagt gcatagctat caatgcctcg ctaatcagat aaagtgctta
    40981 cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga cgataatcag caccatcagg
    41041 gcgcaggttt gctggaactg gaaaccgcga atcgcttccc acagcgtgac gccgatcccg
    41101 cccgcgccga ccatgccgac gacggtcgcc gagcggacgt tggattcgaa gcgatagagg
    41161 gagtaggaga tcagcagcgg catcacctgc ggcagcacgc cgtagaggat ctcttcgagc
    41221 ttgttggcgc cggtggcgcg aatgccttcc accgggccgg gttcaatcgc ttccaccgct
    41281 tcggaaagca gtttggagag cacaccggtg gtgtggataa acagcgccag cacaccagcg
    41341 aatgggccga ggccgacggc gaccacgaac agcatggcga agaccatttc gttaatggcg
    41401 cggcaggcgt ccatcaggcg gcgcacgggc tggtaaaccc accacggcac caggttttcg
    41461 gcgctcatca ggccgaaggg gatggagaga accaccgcca gcgcggtgcc ccagacggcg
    41521 atttgcagcg tgacgagcat ttcggtgagg taatcctgcc actggctgaa atcgggcggg
    41581 aagaagtcgg cggcgaaggt cgccatgttg ccgccgtctt tgatcagcgt gagcggggcc
    41641 atttccgcgc cctgccacga gacgaccaac acggcgagta caacggccca gctcagaagc
    41701 gagaaccagc tgcgcttggg tggggcgatg gtgatggttt gcatgtttgg ctccggtctg
    41761 taggccggat aaggtgcttg caccgcatcc ggcatcaacg cctgcacatt gtcggatgcg
    41821 acaccggggc gtcttatccg acctacgatt tactgcatcg ctttacttac cgaactcatc
    41881 gcgcttaacg cgttgttcag gcggtccagg tcatccagct gcgcttgaat ctcggtggtt
    41941 tttgccagct tgtcctgctc attcagccct ttattgcctt tcacgctttg catctcttta
    42001 aacagtgcga gctggcgaat cggcaccagt tgcaggtcgc tggaggcgcg gaacggcgcc
    42061 cagcccaggc gttccagcac cgctttctct tccggcgtct tgccgtaatt cataaagaag
    42121 tcgtagatct tgtctttggt ggtttcggaa aggtttttac gccagacgat cggatcgcct
    42181 gggatcagcg gtgacttcca gatcactttc agttctttca gcttctccgg cgcggaggtt
    42241 ttcagcttgt cgaggttttc ggtgttgttg gtcgccacat ccacctgctt gttggcgacg
    42301 gccagcgcgt tggtttcatg cccggcgttg acggtgcgct tgaagtcact ggcggagata
    42361 ttgtttttgg agaagacgta gtagccgggg acgaggaagc cagaggtgga gttaggatcg
    42421 ccattaccga aggtgagatc tttccgcttc gccagcagat cgttcaggtt gttgatcggg
    42481 ctgtctttgt tgacgatcaa cacgctccag taacccggcg atccatccgc cgcgacggtc
    42541 tgagcgaaga cttggccgtt ggcgcgatcc actgcttcca tcgccgacag gttgccgtac
    42601 caggcgatat ccactttgtt gaaacgcatt ccctggataa tgcccgcata gtccggggca
    42661 aagaaggcgt tgaccttcac gcccagcttc ttctccatat cctgtaaaaa cggcgtccat
    42721 tgcggtttca ggttttgctg tgattccgtt gaaataatgc cgaaattcag cgccttttcc
    42781 tgctcttcgg cgtgtgccgg gcttaacagg gtgctgaggc tgaacatgct ggtgaaggcc
    42841 agcgaggcaa ttatcttagc gttcattcgt tttcctcaat gatggggatg tcaggcagct
    42901 ttcgcgttct cttcgacgcg gttgatgctg cggtagagat ggtcaaaacg ttcgttatca
    42961 aactgttggc tgctgccgtc gtagaagacg tgcccctggc gcagggcgac gatgcgttcg
    43021 cagtagcgca gggcgtaatc cacctgatgc agcgtgacga ccacggtgat gccgtcgttc
    43081 tggttgatgt cgcgcagggt gtccatcacg atgcgcgccg attccgggtc cagcgaggcg
    43141 atcggttcgt cagcgagaat cactttcgcc tgctgcatca gcgcgcgggc aatcgccaca
    43201 cgctgctgct ggccgccgga gagcgtggaa acacgctgat acgcaaagtg cgccataccg
    43261 acgcgggtca gcgcctgtaa tgcgcgctgt ttctgctcgc gggtgaacca actaaaacag
    43321 gtgcgccaga acggcgtgct gccgagcgcg ccaatcagca cgttctccag tacgctcagg
    43381 cgattcacca ggttgaattg ttggaagatg tagccggtgt tggcgcggct tttgcggata
    43441 tcgcgggcca ggcggccttc gcgctggaca gtgcggccca gcagctcgat atggctaccg
    43501 gcggatttat cgccggtaat caaaccgctt aagtgacgta aaagggtgga tttgccggaa
    43561 ccggacggcc caagcagagc caccatttca ccatgatgga tattcagatc aaccgcgtgc
    43621 agcgcctgat gctggttgaa ggttttgctc agttgttcga cgcagataat cgtttgcatg
    43681 atgcagcctc cctaaaaaag tggctccatc gtggcggatt attgtgacga tttggttaat
    43741 taaaagtgac taacagatga agagttaacg ggaattcgat gacagtgcgg gagggcgggc
    43801 ctcccggcgg attattgcgt tggttgttgt ttgacgacat taatcatcca cggtacgcca
    43861 aatttatcgg tgactttgcc aaagccatgc gcccagaaag tttcctgcca ggccatttcg
    43921 atttttccgt tagcggcaag gttatcaaac cagcgttttc cttcttcgac ctgttgcgaa
    43981 tcgagcacca gcgtaaaacc ggagtagctg gcttttcctg acggtatagc atcgctcatc
    44041 atgatatcgc ttccggcaat gcgcacattg gcgtgggcga tggcggtatc gggaaattgc
    44101 attccggaag ggcagttctc ggcgctgtcc tgcgctgatt ttggcatttc gccgaagctg
    44161 attttataga gcagttcagc gcccaacgta cgttgataat aggcaatcgc gtcggagcag
    44221 ttaccggcaa aagagaggta gggacttaac ggcatgatgg tgacctcagt taagagaagc
    44281 cagttaagtg tagttcgaaa tttatacaga tgagagggaa gcctgataag acgcgcgagc
    44341 gtcgcatcag gcagtcggca ctgttgccgg atgcggcgta aacgccttat ccggcctacg
    44401 gagggtgcgg gaatttgtag gcctgataag acgcgcgagc gtcgcatcag gcatctggca
    44461 ctgttgccgg atgcggcgta aacgccttat ccggcttacc gatcacaata caatcagttc
    44521 tttttcacaa actcagattt cagtttcatc ggaccaaaac cgtcgatttt gcaatcgatg
    44581 ttatggtcgc cttcaaccag gcggatgttt ttcactttgg tgccaatttt cagcatcgaa
    44641 gagctacctt tcaccttcag atctttaatg atggtaacgc tgtcgccgtc agccagcaga
    44701 ttgccgttag catctttaac gatcagctcg tcgctttcct gtgcaggttc tgcgtcgttc
    44761 cattcgtagg cacattccgg gcagatgtac atgccgttat cttcgtaagt gtattcggag
    44821 ttgcattttg ggcagtgtgg taatgacatg tggatttcct caaaagtcag cagggcaaaa
    44881 gcgccaaaaa acggcagatt gccgaaaaag gccgcaatta tacacaaaat ccctgagttt
    44941 gtcgggggta ctgctgattt tagacgacga tcgttttcat ttgaaaggaa acgcaaaaag
    45001 aaaataa
//
LOCUS       DAZFTN010000022        44376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFTN010000022 DAZFTN010000000
VERSION     DAZFTN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..185
                     /locus_tag="ACHKV2_001434"
     CDS             <1..185
                     /locus_tag="ACHKV2_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6939352.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHKV2_001435"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHKV2_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6939353.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHKV2_001436"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHKV2_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHKV2_001437"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHKV2_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="ACHKV2_001438"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="ACHKV2_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHKV2_001439"
                     /pseudo
     CDS             complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHKV2_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(4262..6121)
                     /gene="gss"
                     /locus_tag="ACHKV2_001440"
     CDS             complement(4262..6121)
                     /gene="gss"
                     /locus_tag="ACHKV2_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO6939354.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            6326..7192
                     /gene="yghU"
                     /locus_tag="ACHKV2_001441"
     CDS             6326..7192
                     /gene="yghU"
                     /locus_tag="ACHKV2_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO6939355.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(7315..7563)
                     /gene="hybG"
                     /locus_tag="ACHKV2_001442"
     CDS             complement(7315..7563)
                     /gene="hybG"
                     /locus_tag="ACHKV2_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO6939356.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(7576..7917)
                     /gene="hypA"
                     /locus_tag="ACHKV2_001443"
     CDS             complement(7576..7917)
                     /gene="hypA"
                     /locus_tag="ACHKV2_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6939357.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(7910..8398)
                     /gene="hybE"
                     /locus_tag="ACHKV2_001444"
     CDS             complement(7910..8398)
                     /gene="hybE"
                     /locus_tag="ACHKV2_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO6939358.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(8391..8885)
                     /gene="hybD"
                     /locus_tag="ACHKV2_001445"
     CDS             complement(8391..8885)
                     /gene="hybD"
                     /locus_tag="ACHKV2_001445"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO6939359.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(8885..10588)
                     /gene="hybC"
                     /locus_tag="ACHKV2_001446"
     CDS             complement(8885..10588)
                     /gene="hybC"
                     /locus_tag="ACHKV2_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO6939360.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(10585..11763)
                     /gene="hybB"
                     /locus_tag="ACHKV2_001447"
     CDS             complement(10585..11763)
                     /gene="hybB"
                     /locus_tag="ACHKV2_001447"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO6939361.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(11753..12739)
                     /gene="hybA"
                     /locus_tag="ACHKV2_001448"
     CDS             complement(11753..12739)
                     /gene="hybA"
                     /locus_tag="ACHKV2_001448"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO6939362.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(12742..13860)
                     /gene="hybO"
                     /locus_tag="ACHKV2_001449"
     CDS             complement(12742..13860)
                     /gene="hybO"
                     /locus_tag="ACHKV2_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO6939363.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(14049..14336)
                     /gene="yghW"
                     /locus_tag="ACHKV2_001450"
     CDS             complement(14049..14336)
                     /gene="yghW"
                     /locus_tag="ACHKV2_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO6939364.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(14455..15342)
                     /gene="yghX"
                     /locus_tag="ACHKV2_001451"
     CDS             complement(14455..15342)
                     /gene="yghX"
                     /locus_tag="ACHKV2_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO6939365.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            15499..16539
                     /gene="gpr"
                     /locus_tag="ACHKV2_001452"
     CDS             15499..16539
                     /gene="gpr"
                     /locus_tag="ACHKV2_001452"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO6939366.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(16579..17073)
                     /gene="yqhA"
                     /locus_tag="ACHKV2_001453"
     CDS             complement(16579..17073)
                     /gene="yqhA"
                     /locus_tag="ACHKV2_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO6939367.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            17264..18148
                     /gene="yghA"
                     /locus_tag="ACHKV2_001454"
     CDS             17264..18148
                     /gene="yghA"
                     /locus_tag="ACHKV2_001454"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6939368.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(18420..18845)
                     /gene="exbD"
                     /locus_tag="ACHKV2_001455"
     CDS             complement(18420..18845)
                     /gene="exbD"
                     /locus_tag="ACHKV2_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO6939369.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(18852..19586)
                     /gene="exbB"
                     /locus_tag="ACHKV2_001456"
     CDS             complement(18852..19586)
                     /gene="exbB"
                     /locus_tag="ACHKV2_001456"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6939370.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            19838..21025
                     /gene="metC"
                     /locus_tag="ACHKV2_001457"
     CDS             19838..21025
                     /gene="metC"
                     /locus_tag="ACHKV2_001457"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO6939371.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            21165..21824
                     /gene="yghB"
                     /locus_tag="ACHKV2_001458"
     CDS             21165..21824
                     /gene="yghB"
                     /locus_tag="ACHKV2_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO6939372.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(21864..22763)
                     /gene="yqhC"
                     /locus_tag="ACHKV2_001459"
     CDS             complement(21864..22763)
                     /gene="yqhC"
                     /locus_tag="ACHKV2_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO6939373.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            22957..24120
                     /gene="yqhD"
                     /locus_tag="ACHKV2_001460"
     CDS             22957..24120
                     /gene="yqhD"
                     /locus_tag="ACHKV2_001460"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO6939374.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            24225..25052
                     /gene="dkgA"
                     /locus_tag="ACHKV2_001461"
     CDS             24225..25052
                     /gene="dkgA"
                     /locus_tag="ACHKV2_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO6939375.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            25252..26174
                     /gene="yqhG"
                     /locus_tag="ACHKV2_001462"
                     /pseudo
     CDS             25252..26174
                     /gene="yqhG"
                     /locus_tag="ACHKV2_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            26223..26480
                     /gene="yqhH"
                     /locus_tag="ACHKV2_001463"
     CDS             26223..26480
                     /gene="yqhH"
                     /locus_tag="ACHKV2_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO6939376.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(26523..28742)
                     /gene="ygiQ"
                     /locus_tag="ACHKV2_001464"
     CDS             complement(26523..28742)
                     /gene="ygiQ"
                     /locus_tag="ACHKV2_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO6939377.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(28853..30265)
                     /gene="ftsP"
                     /locus_tag="ACHKV2_001465"
     CDS             complement(28853..30265)
                     /gene="ftsP"
                     /locus_tag="ACHKV2_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO6939378.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(30340..31077)
                     /gene="plsC"
                     /locus_tag="ACHKV2_001466"
                     /gene_synonym="parF"
     CDS             complement(30340..31077)
                     /gene="plsC"
                     /locus_tag="ACHKV2_001466"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO6939379.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(31311..33569)
                     /gene="parC"
                     /locus_tag="ACHKV2_001467"
     CDS             complement(31311..33569)
                     /gene="parC"
                     /locus_tag="ACHKV2_001467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO6939380.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(33707..35314)
                     /gene="ygiS"
                     /locus_tag="ACHKV2_001468"
     CDS             complement(33707..35314)
                     /gene="ygiS"
                     /locus_tag="ACHKV2_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6939381.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(35423..35905)
                     /gene="ygiV"
                     /locus_tag="ACHKV2_001469"
     CDS             complement(35423..35905)
                     /gene="ygiV"
                     /locus_tag="ACHKV2_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO6939382.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(35958..36350)
                     /gene="ygiW"
                     /locus_tag="ACHKV2_001470"
     CDS             complement(35958..36350)
                     /gene="ygiW"
                     /locus_tag="ACHKV2_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO6939383.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36502..37161
                     /gene="qseB"
                     /locus_tag="ACHKV2_001471"
     CDS             36502..37161
                     /gene="qseB"
                     /locus_tag="ACHKV2_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO6939384.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37158..38507
                     /gene="qseC"
                     /locus_tag="ACHKV2_001472"
     CDS             37158..38507
                     /gene="qseC"
                     /locus_tag="ACHKV2_001472"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO6939385.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38617..39198
                     /gene="mdaB"
                     /locus_tag="ACHKV2_001473"
     CDS             38617..39198
                     /gene="mdaB"
                     /locus_tag="ACHKV2_001473"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO6939386.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            39229..39543
                     /gene="ygiN"
                     /locus_tag="ACHKV2_001474"
     CDS             39229..39543
                     /gene="ygiN"
                     /locus_tag="ACHKV2_001474"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO6939387.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39588..40475)
                     /locus_tag="ACHKV2_001475"
     CDS             complement(39588..40475)
                     /locus_tag="ACHKV2_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6939388.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(40472..41419)
                     /locus_tag="ACHKV2_001476"
     CDS             complement(40472..41419)
                     /locus_tag="ACHKV2_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6939389.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(41430..42467)
                     /locus_tag="ACHKV2_001477"
     CDS             complement(41430..42467)
                     /locus_tag="ACHKV2_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6939390.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(42476..43459)
                     /locus_tag="ACHKV2_001478"
     CDS             complement(42476..43459)
                     /locus_tag="ACHKV2_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6939391.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(43456..44265)
                     /locus_tag="ACHKV2_001479"
     CDS             complement(43456..44265)
                     /locus_tag="ACHKV2_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6939392.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT        11146 a        11858 c        11173 g        10199 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgataaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     3961 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     4021 tttaacggtg tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
     4081 gtgtatcaga caataagcat tcattaaaaa tctttagaaa taattagaac acaggcattc
     4141 tgctggttaa tgtattcaca tatgaaatga ataaatattt gaacatataa atattgaata
     4201 ttttgattaa tccccgtact gactattctt cataatcagt acgggattgc tacaacatgt
     4261 attactcttt caccacaatt aacggttcaa tatcactctc ttttttaata accagtgatt
     4321 catcaccgcg caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa
     4381 tgtatttacc gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg
     4441 caaatttacc actggttttg tccagcacct cttcatgatg gctgacgagg tcgatgttgc
     4501 tgccacagcg accggcgatc ggtttcactg cgtaacctgt tttcaccaat tcatcattaa
     4561 cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca
     4621 gaatcgcttt gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc
     4681 gcagcaatac gtcgataaga cgcacttcat tttgcggatg accggtacgg atcggcaccg
     4741 cagcaaactc acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg
     4801 ttttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc
     4861 agcccagttc atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct
     4921 gctccataaa ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa
     4981 acggacgtgc acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg
     5041 gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt
     5101 cggtatgaca ggaggcggag tcggcgttgt actcgtaaac cttcagacca cgctcatcca
     5161 tgcagaaatc catacgaccg gtgatcatat ggtgacggcg acgctgccag gagagacgca
     5221 aacgtggcca gaggattttc ggaatgtcga acagcgccag caggttgtca tctttcagca
     5281 ccttgtcggt tgcgtgaaga tacatcaggt gcagctcgtt ggtggcttta atcagctcct
     5341 gctcggcgct ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt
     5401 tggcctgcac ataggcgttt tgcagcggat ctttttcatc cagccattta ccgtcaaact
     5461 ggcctttgtt ttccagacgc gctccgctga ttttcagcag ctcgcctgca atttccggct
     5521 gcggtaagct gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat
     5581 caaaagtgtc tttaagggta tagcagccgt tttcgaccac catctccagt tcgcgcgtcc
     5641 actgttgccc ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata cggactttgt
     5701 tgccatgtaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat
     5761 cccagataag aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca
     5821 atggcaggat gttgtcatta accacttcac gcaggaagcg cagcgagaaa atctcccacg
     5881 ccatacccac gtcagtaaag accacaccgt agttcagaaa gagaaaacgg cgagcaaatt
     5941 caacgcattg ccatttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt
     6001 catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac
     6061 ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca
     6121 tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc
     6181 tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg
     6241 gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac
     6301 actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg
     6361 gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgg tttctggtcc
     6421 gacgcatgaa aaaacgctgc cggtgggcaa acatccatta caactttatt cgctgggaac
     6481 gccgaacggt cagaaagtaa cgattatgct tgaggagctg ctcgcgctgg gcgttactgg
     6541 cgcagagtac gatgcctggc tgatacgcat aggcgatggt gatcaattct ccagcggctt
     6601 tgtcgaagtg aacccaaatt cgaagatccc ggcgctgcgc gatcataccc ataatccgcc
     6661 gatccgcgtg tttgagtcag gatcgattct gctttatctg gcggagaaat ttggctactt
     6721 cctgccgcag gatctggcaa aacggactga aacgatgaac tggctgttct ggttacaggg
     6781 cgcggcaccg ttcctcggcg gtggttttgg tcacttttac cattacgcgc cggtgaaaat
     6841 tgagtacgcc atcaatcgct ttaccatgga agccaagcgc ctgctcgacg tgctggataa
     6901 gcaactggcg cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat
     6961 ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat gatgccgcag agtttctgga
     7021 tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa gtgggcgaac gtccggcagt
     7081 gaagcgtggg cgtattgtta accgcaccaa cgggccgctg aacgagcagt tgcatgagcg
     7141 ccatgacgcc agtgattttg agacgaatac ggaagataag cgtcaggggt aaggattggt
     7201 gttcgtcgca gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgag
     7261 cacaatgcct gatgcgacgc tgccgcgtct tatcagacct acgaaagcta atcatcacgc
     7321 gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc
     7381 gtcgatgatg ctcatggcaa atccgacgtg taccagcacc cactggccca gcagatcggc
     7441 agggttacct tcacaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac
     7501 ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca
     7561 cattgttaac tccggttatt caacttcaat acttttgacg atcagcgaat cgccggtatc
     7621 gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaacctccac
     7681 cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca
     7741 cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc
     7801 aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg
     7861 ctccgcctgc cgctggataa tttcaacggc gctctggcaa agcgacaact catgcatttt
     7921 cgccactcct gcgaccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattcg
     7981 tgactggcag cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccctcttcaa
     8041 tcgacatact gtgcgaaagc ggtgacatca gcgaacagga gaggtattgc gaaacaccgt
     8101 ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac
     8161 tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc
     8221 acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca
     8281 ccggcattga aggatgcaga aaagagagag cgtgcatcga acgccgggca atttcttcaa
     8341 acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga
     8401 atcgcctccc gtgggatggc ttccacgcca gattcacgca gcgcagccag aacctgctca
     8461 agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat
     8521 tccggaatta cgccgaccag ggtcagcttt ttcggaaact cgccggtgaa gcgcagggcc
     8581 gacaagacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga
     8641 acttcttcat cccgcaggat catcatcgtt cccggcgcgc tcttcttcga cacaatggca
     8701 tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg
     8761 ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca
     8821 atccgcacac cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata
     8881 cgcattacag aaccttcact gaaaccactt cgttgccgtc agcatccact acgtgtaccg
     8941 cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg
     9001 gatcagcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt
     9061 tacgcgggcc agagttccag gttgatggaa caactgcctg gtagttgctg atgataccgt
     9121 ctttgatcac catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt
     9181 tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga
     9241 tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa
     9301 cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc
     9361 cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac
     9421 ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga
     9481 aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa
     9541 tggtggtacc ttcccacggt gcctgcggcg cttcgtcttt ataccaggag tgcttcgcgc
     9601 tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg
     9661 acagatccgc attctcaatg tagccgcccg ggaacaggaa gctgccgttt ttactgtcgg
     9721 tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg
     9781 ggtagaacgc agcgataacc gcagtatcga ccttgtaaac ctgctcaaca aagtcactca
     9841 gcttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcagcacg cccagaccgt
     9901 cgaggttgat tgggttagcg accccaccta ccgccaggtt ctgaatgtgc ggcgttttac
     9961 cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc tgcaggtagt
    10021 gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc
    10081 cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt
    10141 tggtgaactc ttccggactg ttcaggtgcc aggtcgaaac acctttcagc atttcggagg
    10201 ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct
    10261 gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt
    10321 attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca
    10381 gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg
    10441 gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt
    10501 tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta
    10561 ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt
    10621 ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc
    10681 aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc
    10741 gccgcccggg ttgaatgcca ccagcgaata ggtcagacgc caggttgcac aacctaacag
    10801 cgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag
    10861 aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc
    10921 accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcacgat
    10981 gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaataggc tcttttcatc
    11041 cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa
    11101 gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccacag
    11161 cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt
    11221 cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gctgtagcga
    11281 caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat
    11341 gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg
    11401 aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc
    11461 cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc
    11521 acggttaaag acgtataccg cccacgcaag cgcccagccg ccacaggcaa agccggtgcc
    11581 aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac
    11641 agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg
    11701 tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact
    11761 catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtca
    11821 agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt
    11881 cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca
    11941 cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat
    12001 aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt
    12061 ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac
    12121 gcgtaccaaa aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt
    12181 tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg
    12241 ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac
    12301 cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag
    12361 agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt
    12421 acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacttgaa
    12481 tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg
    12541 ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc
    12601 acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag
    12661 ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa
    12721 taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg
    12781 acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc
    12841 gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt
    12901 ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc
    12961 gataccttct tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc
    13021 aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag
    13081 gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc
    13141 agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt
    13201 cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt
    13261 cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg
    13321 cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc
    13381 ccacgcagag caggaaccga tagcgataat ggctgctgcg ccttccgccg ctttgcggat
    13441 gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat
    13501 ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt
    13561 ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatactcca gagagatagt
    13621 ctccagtacg aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc
    13681 ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc
    13741 ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag
    13801 cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat
    13861 ttatagttat tccgttgcga agacctggca tatattttgc ctcaatcgca aaatcaataa
    13921 tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg
    13981 cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt
    14041 atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa
    14101 ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga
    14161 atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag
    14221 tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg
    14281 tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt
    14341 cacctcattc gttatatatc gtaatatata gcgatatttt aggcaaagaa cgacttttgc
    14401 gcacgctatt ttttcatgct tgtttaagac gtaatcccgt aagagataaa cctatcagga
    14461 gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc
    14521 ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga tatacgcctc
    14581 ataaacctta ttattggctt tcaacgccgc ctcgtaagca ggccagccct cgttgattcg
    14641 ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt
    14701 gggtgcctga cgaccataaa acggcactgc gcaggccagt tccgggtaag cgaccgctgc
    14761 cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttgc ctgtcgcttg
    14821 cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc
    14881 aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc caacggaatt
    14941 taagccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc
    15001 gatatacgga ttcagtccac gattctcatg caccaccacc acggctggcg ttttgccgct
    15061 catttttgcg ggtttaacca gataaccccg tacctcgccg tgaccatttg gcgaaggata
    15121 cgtgatgtac tcagcaaaaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata
    15181 atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcctaccg catacttcgc
    15241 cgcaaggttg aggaactcac gtttcgagat tttcccgtga gcgtaatagt cgtagtaatc
    15301 cagcaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcac tcctcagttg
    15361 gtgttttttt aagcaaagca taagcacgtg tttttgccca gtttttcgtc actctgtgag
    15421 ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa
    15481 cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcaa
    15541 taccgctatt gcggaaaaag cggtttacgc ctgcccgcgt tatcgctcgg tttatggcac
    15601 aatttcggtc acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa agcgtttgat
    15661 ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa
    15721 gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga actgattatc
    15781 tctaccaaag ctggctacga tatgtggccc ggcccttacg gctctggcgg ttcacgtaaa
    15841 tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc
    15901 ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct
    15961 catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc accagagcgg
    16021 acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgctgttaat tcatcaacct
    16081 tcgtacaatt tactaaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat
    16141 aacggcgtgg gctgcattgc ctttactcct ctggctcagg gattgctgac cggaaaatat
    16201 ctcaacggca ttcctgaaga ttcacggatg catcgtgaag ggaataaagt tcgtggtctg
    16261 acgccgaaaa tgctcaccga agccaacctc aacagcctac gcttattgaa tgaaatggca
    16321 cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgagcga
    16381 gtgacgtcgg tattggttgg tgccagccgc gcggagcaac ttgaggagaa cgtgcaggcg
    16441 ctgaataatc tgacatttag caccgaggag ctggcgcaga tcgatcagca tatcgccgat
    16501 ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa cgggcgcgag
    16561 aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccattac
    16621 aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatcagg
    16681 gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga
    16741 cgctgctact ttgtttttca gcgacgttgc atccattttc cccagccagt tcagcttctc
    16801 tttgttctcg gagatatcga gttgtgagac gaaattctca taaccggaaa acatcaccat
    16861 caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc
    16921 tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag
    16981 cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga
    17041 agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg
    17101 caacagtata tcgcaattga gtaaacagcg cgcaacagca aatcaacata tccgttaaat
    17161 ctttgtcgca taattctgag ggttagggat cttcctggtt cccttatcaa cgcatcgaga
    17221 gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga
    17281 cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat
    17341 ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg gtagcggtcg
    17401 cctgaaagat cgtaaagccc tggtgacagg gggcgattcc ggaatcggtc gcgctgccgc
    17461 catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga
    17521 agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc
    17581 aggcgattta agcgatgaga aatttgcccg ttcgctggtt cacgaagcgc ataaggcctt
    17641 aggtgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc ctgatattgc
    17701 agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg
    17761 gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtattatca ccacttcgtc
    17821 aatccaggca taccagccaa gcccgcattt actggactat gcagctacga aggcggcgat
    17881 tctgaactac agccgaggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat
    17941 tgtcgcgcca ggcccgatct ggacagcact gcaaatttcc ggcggacaaa cgcaggataa
    18001 gatcccgcag tttggtcagc aaacgccgat gaaacgtgcg gggcaaccgg cggaactggc
    18061 ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg
    18121 cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca
    18181 tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaatgcat
    18241 gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt
    18301 gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg cacgaagatt
    18361 caacatgttg caatagattt tgtaggcctg atgaacgtag cgcatcaggc aattctactt
    18421 tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc
    18481 agcgtgtcca ttaccttcat caacgtctcg taatcgacag ttttatccgc tcggaagaag
    18541 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    18601 gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt
    18661 ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttaacctt cacatctacc
    18721 gtcgctaacg gtgccgccac cataaagata atcagcagga ccaacatcac gtcgataaac
    18781 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt
    18841 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg
    18901 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc gacatcaccc
    18961 agcatcgctt taaagccgcc aatctgacgc gcgaatacgt tatagataac gaccgccgga
    19021 atcgctgcca ccagaccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg
    19081 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    19141 accgtaccaa acagcccaac gaacggcgaa atcgcgccga tggttgccag atagccgtta
    19201 ccgcggccca tttgacgacc tactgcggcg acccgacgct ccagacggaa gctggtacgt
    19261 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    19321 ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    19381 tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgatta
    19441 aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc
    19501 ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg
    19561 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc
    19621 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    19681 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    19741 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    19801 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    19861 tcaactggtg aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat
    19921 tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa
    19981 tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca
    20041 acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc
    20101 agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    20161 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    20221 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    20281 taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc
    20341 gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg
    20401 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaagccgc
    20461 caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg
    20521 ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac
    20581 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga
    20641 aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca
    20701 ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag
    20761 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    20821 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    20881 ggcaaatcaa ccagaacata tcgcagccat tcgcccacaa ggcgagatcg attttagcgg
    20941 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    21001 cgccggtttt gcgcggattg tataacattg ccacttttgg acaattttgc agacatttta
    21061 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    21121 caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    21181 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta
    21241 gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct
    21301 catttttgcc aggcgacagc ttgttgatat tggctggcgc attgattgcc cagggggtta
    21361 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    21421 gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    21481 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    21541 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    21601 ccggtctgcc aaaccgccgt ttccagtttt tcaactggtt aagcggattg ctgtgggtca
    21661 gcgtggtaac cagtttcggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag
    21721 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag
    21781 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccctgttcc
    21841 cggttgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    21901 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    21961 tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc
    22021 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    22081 aaattatggt ggaacgccga tacgctcata ttagcttctg ccgccagttg ctcgacgctc
    22141 aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag actgaagtga
    22201 gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    22261 agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg cgccatcacg
    22321 tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg
    22381 ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tatccatcaa cagttcctgt
    22441 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    22501 gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
    22561 aacacgcgtt cattgatata accgatttta tgtccggaaa agagaattat gatgccaggc
    22621 tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc
    22681 aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag
    22741 cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat
    22801 tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga
    22861 aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc
    22921 tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc
    22981 ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac
    23041 gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg cgttctcgat
    23101 caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac
    23161 ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc
    23221 ctgctggggg ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct
    23281 aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa
    23341 agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgcc
    23401 ggtgcggtga tctcccgtaa aaccacaggc gataagcagg cgttccattc agcccatgtt
    23461 cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg
    23521 gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt
    23581 gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat cgaagatggt
    23641 ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg
    23701 acgcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc aacgcatatg
    23761 ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc
    23821 ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct gcaatatgct
    23881 gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc
    23941 gcaacccgca atttctttga gcaattaggc gtgcctaccc acctctccga ctacggtctg
    24001 gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg
    24061 ggcgaaaatc atgacattac gctggatgtc agccgccgta tatacgaagc cgcccgctaa
    24121 gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac
    24181 ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg
    24241 ttattaagct acaggatggc aatgtcatgc cccagttggg actgggcgtc tggcaagcaa
    24301 gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgtttg
    24361 ataccgccgc ggcctataag aatgaagaag gtgtcggcaa agccctgaaa aatgcctcag
    24421 tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac aatcgccccc
    24481 gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa
    24541 tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat
    24601 tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc
    24661 aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc
    24721 tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct
    24781 ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg
    24841 cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc
    24901 tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg
    24961 atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc
    25021 tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt ggcggtaatg
    25081 ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc
    25141 ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa
    25201 agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata
    25261 atacttctgt ttttagcagc cctggcaagt tttaccgtgc acgcacagcc cccctcacag
    25321 accgtagaac aaacagtccg gcatatttat cagaactata aatcagatgc cactgcccct
    25381 tattttggtg aaaccggaga gcgtgcgata acttctgtgc gtattcaaca ggcgcttacc
    25441 ctgaacgaca atcttacgct gccgggtaat attggctgga ttatgatccg gtttgtgatt
    25501 gtcaggattt tggcgatctg gtgctagaaa gcgtagcgat aaccgaaact gacgccgatc
    25561 atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacgcaga
    25621 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg
    25681 gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt
    25741 tgcaaaaaga acagccggaa gcctttgttg ccgaactctt tgaacatatt gccgattata
    25801 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggtcaat gccttctata
    25861 aaaccacctt caagacggcc aacaatcccg atgaagatat gcaaatagaa cggcaattta
    25921 tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc
    25981 gagtcctgga gaaaaccgcc gattccgccc gcattcatat tcgttttacg ctgaccaatg
    26041 gcaacaacga agagcaagaa ctggttttac aacggcgcga aggtaagtgg gaaatcgctg
    26101 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca
    26161 gattaaagca atgagctgaa ttaaatagca attagccgga acaataaaaa agggaacact
    26221 atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcgtg ctcagtggct
    26281 gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt gcaaacatta aatgccaaaa
    26341 tcgcccggct tgagcaggat atgaaagcac tacgcccaca aatctatgct gccaaatccg
    26401 aagctaacag agccaatacg cgtcttgatg cacaggacta ttttgattgc ctacgctgct
    26461 tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc cggggatttt
    26521 ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac cagcattcac cggacgagat
    26581 tgcgtagacg acgctttttt tgccgtagca ggcgtctgac actgggtcgc catcggcgta
    26641 tgtttcgtca acgccggacg ggtattgcgg ttctggcgac gagcctcacg catctcttca
    26701 atggttggcg caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc
    26761 gcttccagcg cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct
    26821 ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga cgtcttcact cttataacca
    26881 atcttcgcca gcgggttttt cccggtgtaa tacatggtgg tcgagttagc cagtggcgat
    26941 gggtagaagt tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaaa
    27001 ttcaccatat cttcatcacg cgtaccgggg tgcgcggaga tgaaataggg gatcagatac
    27061 tgctctttac ctgcctgttt cgagtaggta tcgaacagct ctttaaagcg gtcataactg
    27121 cccatgcccg gcttcatcat cttcgacaac ggcccttctt cggtatgttc cggagcaatc
    27181 ttcagataac cgccgacgtg atgggtcgcc agttctttga tatagcgcgg atcttccacg
    27241 gcgatgtcat aacgtacgcc tgaggcaatc agaatctttt taatgccttt cagatcgcga
    27301 gcgcggcgat agaggttgat cgtcggttcg tggttagtgt ccatatgcgg acaaatatcc
    27361 gggtaaacgc aggacaaacg acggcaggtt tgttcagcgc gtggcgattt gcagcgcaac
    27421 atatacatgt tggcagttgg cccaccgaga tcggaaatta cgcccgtaaa acctggaacg
    27481 gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt cggaacggct ctgaataatg
    27541 cgcccttcgt gctcggtgat agaacagaaa gagcagccgc caaagcagcc gcgcataatg
    27601 ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg cattgccata ggccggatgt
    27661 ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct cttcggtaga aagcgggata
    27721 gcaggcgggt tgatccacac atagcggtca ccgtgttttt gcatcaatgc gcgggcgcaa
    27781 cctgggttag tttcgtggtg cagaatacgc gaagcatggg cgtacagcac tttatcgccc
    27841 ttcactttct cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt
    27901 ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg
    27961 cacggcaaat cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga
    28021 cgggtggaat ccacgccgct ccagccaggc agcgcctctt tcacgataat cgcggtatta
    28081 cgcacatcgc ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc
    28141 agcggacgct caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa
    28201 cggcgcacgg tatcggacca gtaatcataa tgcgcggtac ggcgcagact agcctcaata
    28261 ccaccgagga tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt
    28321 gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt
    28381 aaacggcgat cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg
    28441 aaaaacagat tcggtttacc cagacgcatg aaatcgtctt tgctgctcca gtctggctgg
    28501 gcgatgatcc cgacgcgaaa gccctgtgct tccagcatac gaccgcaaat cgccatcccg
    28561 aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag
    28621 ccaagttgat ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg
    28681 gcccagtact gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc
    28741 attttgcttc cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga
    28801 aagagaaatc gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta
    28861 ccggattgac taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
    28921 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
    28981 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcaccat
    29041 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
    29101 gtaccgtcca gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc
    29161 acagttgtcc attaataccc gggtcatcac ccagactgat atcgcgactg cgaatcggcg
    29221 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
    29281 gaaggccggt tgggcgcagc gttagcacca gggtagaaac cagaatgctg gatggctcaa
    29341 agaagccacg aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat
    29401 caccgttgct catatccacc agaatctcgc ggcgctcgcc cggtgccaac gaaagttgct
    29461 tcaccgatac aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
    29521 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca
    29581 cccagccacg cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac
    29641 caacaaagcc gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt
    29701 tatcctggat aatgaccgga aaatcatcca caccataatg gttggggata ggcagcgact
    29761 tgctgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
    29821 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga ataggcagta
    29881 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggcctg
    29941 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
    30001 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
    30061 cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
    30121 acagcggttg cccacggcga gattcaagca gcggcggaac gggtagcggt tgctgttgcc
    30181 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
    30241 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
    30301 tgcaggaaag tgtttcccgc aaaaatacag attgccgact taaacttttc cggcggcttc
    30361 gcgctctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
    30421 ggcagccagc tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
    30481 ttcgacaatc accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtgga
    30541 gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ccccagtctt
    30601 gaacggtagc aggccgcgac cacggctgcg ggtaccttcc gggaacatcc agatggaaat
    30661 acggcgtttt ttgaaatgat tcactacttc cgcaatagtg ccgtgagctt tagtgcgatt
    30721 gtttctgtcg atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca
    30781 cagcaagctc tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac
    30841 catgtcatag ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc
    30901 tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc gcaagacgac caaacatatg
    30961 cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa
    31021 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
    31081 accctctgaa ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg
    31141 ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt
    31201 tcaagggaat ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt
    31261 tcatccggcg ttccttgcaa gcgggaggaa acagcgccct ccccggcata ttactcttcg
    31321 ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa cacgatcgat acgctgcaaa
    31381 ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga ctttctgtag ctcttccgga
    31441 cgcagtttaa ttttgcgttt cccaacatga atggtcagcg tgctttgcgg cggcagaacg
    31501 tacagttgcg ccagaccatc ctcgccacgc gcggcttctg ccgatggaat gttgataatc
    31561 ttgttgcctt tgcccttcga cagctgcggc agatcactta ccgggaacat caacatacgg
    31621 cctgcctgag tgattgccag cagcatatcg gaagcatctt caattaccac cggcggcata
    31681 acatgggcat tttccggtaa ggtgatcaaa gccttacctg cacggttacg cgccaccaga
    31741 tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag ccatcagcag tttctgatcg
    31801 tcgctttcca tcagcatatg gtcaacggtc gccccaggcg gcaacgttaa tttgccggtg
    31861 agcggttcgc cctgaccacg cgccgacggc agcgtaatcg gatcaatggc atagctacga
    31921 ccggtggaat caacaaacac taccggttgg ttgctcttac ctttcaccgc cgctttgaag
    31981 ctatcacccg ctttataatt caggcccggc gcgtcgatat catggccttt agcgctgcgt
    32041 acccagccca tctgcgacag cacaatggtg acaggttcag acggcagcat gtcgtgctcg
    32101 ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac gacgatcgtc accgtaggct
    32161 tgcgcgtctg cctgcagttc tttcttcagc aggttattca ttttacgctc ggaagccaaa
    32221 atgccctgca actggtcgcg ctctttttcc agctcactct gctcaccgcg aatcttcatc
    32281 tcttccagtt tggcaagatg acgcagtttc agttcgagga tcgcttccgc ctgggtttcc
    32341 gtaaggccaa accgcgacat cagcgccggt ttcggttcat cttcattacg aatgatctca
    32401 atcacttcgt cgatattgag aaacgccacc agcaaacctt cgaggatatg caggcgcttg
    32461 aggactttct ccagacgata gttcagtcgg cggcgcacgg tatcgcggcg gaacaccagc
    32521 cattcggaga gaatttccag caggtttttc accgccggac gaccatccag accgatcata
    32581 ttaaggttaa tgcgatagct cttttccaga tcggtggtag cgaagaggtg gttcatcacc
    32641 tgatccatat ccacgcggtt ggaacgcggc acaatcacca gacgggtcgg gttctcgtgg
    32701 tcagattcat cgcgcagatc gtcaaccatc ggcagctttt tgttgcgcat ttgcgcagca
    32761 atttgctcca gtacgcgcgc acctgaaacc tgatgcggca atgcgctgat aaccaccgcg
    32821 ccatcttctt tcttccacac cgcgcgcata cgcactgaac cacgtccgtt ctcgtagatt
    32881 ttacggatct cggcgcgcga agtgataatt tccgcttcag tcggataatc cggcccctgc
    32941 acgatatcca gcagctgatc gagcgtggtt ttcggctggt cgattaatgc gattgccgcc
    33001 tgagccactt cacgcaggtt atgcggtgga atatcggtcg ccatgccgac ggcaataccg
    33061 gtggtgccgt taagcaaaat gtttggcaga cgggcaggta gcattttcgg ctcctgcaaa
    33121 gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc ccaactcgct caatagcagc
    33181 tcggaatatt tcgacaaccg ggattcggtg taacgcattg ccgcgaacga tttcggatcg
    33241 tccggcgcgc cccagttccc ctgaccatca accagcggat aacggtaaga gaacggctgc
    33301 gccatcagga ccatcgcttc ataacaggcg ctatcgccgt gcggatggta tttacccagt
    33361 acgtcaccga cggtacgggc cgatttttta aatttggcgc tggcattcag gcccagttca
    33421 gacatcgcat acacaatgcg gcgctgaaca ggtttcagac catcaccaat aaacggcaac
    33481 gcacggtcca tgatcacgta catggagtag tttaagtagg cgttttccgt aaattcatgt
    33541 agcgcaaggc gctctgccat atcgctcatt aattctgatt cctcaactta ttcgccagcc
    33601 ttcaaagggg caatattgcc ggagatacta cattatctgc cgcgtcgagt cacaggagat
    33661 cagcgtgaat agcgccgccg gacgtgctcc gacggcgata aaggcatcaa tgtgccttga
    33721 tatacaactc tttgctgtag acataatcct ggggattatg cagcggaaaa ccgccaacgt
    33781 agggtttaag cagtttgatt aacggctgat agtagatagg aatcagcggt gcctgctggt
    33841 tgacgatcac ttctgcctgc tgatacaacg cattacgctt tgtcgcatca gtgatctgca
    33901 tggcctggtt tagtaaggcg tcatactgcg catttttcca gtgaccgacg ttttcttcac
    33961 tatcgctttt gagcgtgttc aggaagctgg aagcatcatt gtacgtcgca tcccacgact
    34021 gccgagacag catgaaatca ccggctcgtc gggcatcaag ataggttttc cactccattg
    34081 tgcgcagcgt cacctgtgca cccagccatt ttttccattc ggaagacaac gctatcgcgg
    34141 tcttttcatg cagatcgtac ttgttgtaga acagctcaaa gcgtagcgga tgagaggcgt
    34201 cgtatcccgc ctgtttcagc aaggcttttg ccatcgcgac gcgctcactc attggctttt
    34261 gcagttcatc gaacgtcgtc gcgctaaagc cttttacctc tggcggcgtc agcgtggttg
    34321 cgggcgttct caaccccagt accttttgcg caataagctg tcgatcaacc gtaagatata
    34381 gcgcccgacg cactcgcaca tcgttaaatg gcggtttctc aaggttgaag ttgtaatatt
    34441 cgctgttcag acgcggaata attcgtatct cgccaggcag tgatttttca atggcgggaa
    34501 tttgctgcgc cggaacccag gtgagatcga cctctcccgc gcgatagcgg ttatagccgg
    34561 tgaccgaatt atccagcgcc agatactcaa cctgttgcaa tactgtatgt tgcgcatcgc
    34621 ggtactttgg atttttgcgt gcagtaatct tttcgttaac tacccactga tcaagcacaa
    34681 aggcaccgtt gtaaaccatg ttctctggct tactccagct atcgccatgt ttagcgatga
    34741 catgatgagg aaccgggaac agcgtcggcc aggcgagcat cgtcgtgaac caaggaaccg
    34801 gctgctcaag cgtaacttca agagtacgat catccgtcgc tttgacaccc agcgatgtaa
    34861 catctgcttt acccgcaaca atagccgcgg cattgttaat gtgcgcctgt gccagatagc
    34921 cagcaaaagg gcttgccgtt ttcgggtcaa ccgcgcgctg ccagccgagg acaaaatcct
    34981 ctgccgtcag aggctgaccg tcggaccact gcaaaccgct acgcagatgg aaaatatagc
    35041 gcctgccgcc gtccagtatc tcccagcgtt cagcctgagc gggctgcacc tggccttcac
    35101 cgtccatcca taccagacct tcaaacagat ccagcacaat ctgcgcggca gtattctcct
    35161 ccaccttttg cgggtcgagc gtacctgggt cgctatgatt gttgtaacga aagacttgtt
    35221 gcggggcgag ctgtgtgttg gtgggaacgt cagcagcgta gagaggagcc gcacttacca
    35281 ggctgaccag ccataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa
    35341 gcccaacaac aatacgcctg tggcattgtt cgctcaaact tatcgcgagt gatttggctc
    35401 acactccggt aaagattccc catcacgcta acggcacata aacattcgtt tccagtcgct
    35461 gctccgtcac cccttcggca agattggtgt agtgaaacag aatcggtgct ttacgcattt
    35521 tctcgccgct tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt
    35581 ccagctcgcc aacgtggcga gccacggcat aacgtccacc ggtaagttca ccattgctca
    35641 caccataacg attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg
    35701 cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa
    35761 caggagaaag ccccgtctct ttacgccaca tgataaattt cgccacgctg tagttgagca
    35821 actcaggcct acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt
    35881 ttacatccag tgtcaggttt gtcatgttgc ctccctgcga aattattccc gggaacgcat
    35941 cccgggagcg gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca
    36001 gaattccagt ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc
    36061 acgccgttcc agcgtttgtg gtcgatatca acattgatag taccgctggc atctttgaac
    36121 acgtagagat cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg
    36181 tcacgcaggg attttgcgct ttctacagtc gttacgctgc cgttcggccc ctggaatcct
    36241 ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc
    36301 ggtgcgctgc acagggccat tactgcgatt actgctgcga attttttcat gtttattact
    36361 ccctttaatg tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa
    36421 aaaaataaaa tttagtgctg tacagagcgc gttacaacac ggtttactag cagcaaatac
    36481 ggttatcgca gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggc
    36541 gacggcatca aaacgggcct tagtaaaatg ggttttagcg tcgactggtt tacacaaggt
    36601 cgtcagggaa aagaggcgct ttatagcgca ccttatgatg cggtgatcct ggatttaacc
    36661 ttaccaggca tggatggtcg cgatattttg cgcgaatggc gagaaaaagg tcagcgtgag
    36721 ccggtactga tcctgaccgc gcgcgatgcg ctggaggaac gtgtagaagg gctgcgtctg
    36781 ggagctgacg attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggaa
    36841 gctctgatgc gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgtcatg
    36901 ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa
    36961 gaatttgccc tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg
    37021 attgaagaga aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat
    37081 gtgcatcatc tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc
    37141 tacacattag gtgagaaatg aaatttaccc aacgtcttag tctgcgtgtc aggctgacgc
    37201 taatcttttt aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac
    37261 aaacaacgga taacgtcgat gaattgttcg acacccaact gatgctgttt gccaagcggt
    37321 taagtacgct cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata
    37381 aattaaaaca cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg
    37441 gcagaatggt ccttaacgat ggcgataacg gagaagatat tccctatagc tatcaacggg
    37501 aaggttttgc tgacgggcaa ctggtgggcg ataaagatca atggcgtttt gtctggatga
    37561 cctcacctga tggcaaatat cgcatcgttg ttggtcagga atgggaatac cgtgaagaca
    37621 tggcgctggc gattgttgcc gggcaattga tcccgtggtt ggtcgcactg ccggttatgt
    37681 taatcatcat gatggtacta ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg
    37741 cactacgtat gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg
    37801 aagtgcgacc actggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg
    37861 ttcgtgaacg acgctttacc tccgacgcag ctcacgaact tcgtagcccg ttaacggcgc
    37921 tgaaagtgca aaccgaagtt gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag
    37981 cactgctcca attacattcc gggatcgatc gcgctactcg tctggttgat caactgctca
    38041 cgctatcgcg gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag
    38101 atctcctgca atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg
    38161 tgcgactgac actcaatgtc cagggcatca aacgcaccgg acaaccgcta ttgctaagtt
    38221 tgttggtgcg aaatttgctg gataacgccg tgcgttacag tccacagggc agcgtggtgg
    38281 acgtcacgct gaatgccgat aatttcatcg taagggataa cggccccggt gtgacaccag
    38341 aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc gctaccggca
    38401 gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatggcatg aatgttgagt
    38461 ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaaggg taagactttt
    38521 gctaaattcg caaaagactt tgcacatttt gctaatttca ccgtaccgct ctgtgacgta
    38581 ctatagtcgg caaacgtctc accttgaggt taaaaaatga gcaacatcct gattatcaac
    38641 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    38701 gatggcacac tgcgcgacct cgggcatgat gttcgcatcg ttcgcgccga cagcgactac
    38761 gatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    38821 ggctggtgga tgggcgcgcc gtggacagtg aaaaaataca ttgatgatgt attcaccgaa
    38881 ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac
    38941 ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt ctctgacctg gaacgcgcca
    39001 atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat
    39061 ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct
    39121 aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt
    39181 gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta
    39241 atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt
    39301 gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg
    39361 gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag
    39421 cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa ccccgcacat gaaggcgtat
    39481 agcgaagccg taaaaggtga cgtgctggag atgaatattc gtattctgca gccagggatt
    39541 taacattcct gaaaagaggc cggtgttgct accggcctca ggtcagatta gccattcttt
    39601 cttcccgttt tcagcccccg atggaaatcg ttaattcgct taatggtttt aaccgcacgc
    39661 tgcggcaccg cggaagccac cgaccagaca atcatttcca ggcacaacaa taccgtacca
    39721 tgtagtggcg ttttcccctt ttcatccccg cgcggaacat gaatcacgat actggcgtct
    39781 ttgctaaaac gtgaatccag cgcgtttgtc agcaaaataa cagggatccc cagccgtctc
    39841 gcttcacgca gcgttgtcag cccttcccgg tgcgcagatt tttgcgccat catgatcaga
    39901 acatcgccac gttgaagcgc aatcagttgc tcggcaaggc caatacccgt acggtttagt
    39961 gccgtggcag gcaaacctat gcggttgaat agtcgtgcgg tatagtccgc cagaataccc
    40021 gaagcgccaa tgccaaaaat tgcgacttgc cgtgcctgta ccaacaacgc taccgcttgc
    40081 gcaagcgcgt ggcggttttc aggctctgac aggacattac aggtatacaa atgcccttca
    40141 agaacaaagt cgattgcggt gttaacatcg ctcgtcaagt tactcaccgt cgtagacatc
    40201 ttttcgctgg aactaagggc tggaccaagc cactgttcca gagtgcgttt cagatcacgc
    40261 agcccggcaa accccagggc ctgtatagcc ctgataactg tggcatcgga agtgttcaac
    40321 gtggccgcaa tttccatcgc cgtttgatcg agtaccactt cacgatgctc attaatatag
    40381 ctggcgacag ctcttaaccc aggcgttaac tgactcgcgc gagtgcgaaa acgctcacca
    40441 taaatatcaa cccgcgtttt gtctttctga atcatttggc ctccggtaaa ggtcgccccg
    40501 caatttgtga ttgtatctgc gcagccatca gccccagaga tgcaaaggag ttagaaattg
    40561 cctcttcacg agaaatcagc acataacgcc cggaacgaca ggcagtcaga aactgacacc
    40621 atcccggcat gactttttcc attgctgcca gctcatcctg aggtttacca ccggtgtcac
    40681 cgcgccaggt tgcaaaaaca aaatcggcat ccagttcagg taaacgctcc gcgctcacat
    40741 ccattcgccc accttcagga atgctctcaa tcagcggcgg aaagcgaaaa ccagcatcgc
    40801 gtaatacccg ccccagcgaa tgatagctat gcatcacatt aatttttccc tgatttgcct
    40861 gaatcaccga taccgttatt ttttggctgt ccagcgtcgc cttcagcgca ttgatttgtg
    40921 cctgatagcg gcgttcaagt atcgccagct gcgactgcgt tcctgtcagt tctgccagct
    40981 tgcgatagat ttctggcgcg ccccctttga gatgatcaat gctcaccgtg ggcgcaattt
    41041 tttccagtcg ttcaatgggt gtattacggg tcggttctgt gatgatcagg tcaggctttg
    41101 ccgccacaat cgcttcaata tcaatatcag cggtgccaat aaaagcgatc gatgaattat
    41161 caaagtcgac acctgtcaat aacgcgcctg atcgaatgaa atggctaccg tcaggccgag
    41221 ttcgcccgtg gctggcgaca ggaggtacac cgagctcaat cagaggaata gtgatatcaa
    41281 gatcgtgtaa agagacaatt ctctttggat gagcaggaac agtgactttg cgccctaaat
    41341 catcagtaaa gacctgaaca ggttctgtcg cgcgggcaaa gaatgacaac agtatcagca
    41401 gagaaaaaaa taaacgcata taactcccat taaaaactgt tcctgcgctg ccagagcagt
    41461 agcaaaaaga atggtccacc aatcaatgaa ataatgattc cggcaggcag ttgcaaaggc
    41521 aaaaaagcca aacgaccaat gctatccgcc aggatgacta acaacgcccc cgatacggcg
    41581 ctccccagca gtaatgtcgt ctgcccgcca cgtagaataa aacgtgatat atggggtgct
    41641 atcaatccga caaaaccgat gttaccaaca cacgaaacgc agacggctgt caggattatt
    41701 ggtgcggcga cacgtaacaa cgccagacga gaactattca cccctaaccc ggttgcaacc
    41761 tgatgaccaa gcagagcgat atccgccgtc ctggcggtga acagcaataa aagagcggct
    41821 ggcaacatcc aacaggctga aataccgact aacatccagt tagcagcatg caggcttccc
    41881 gataaccaca tcaatgcagt ttgtacatct cgaacatctg cagtggtcat aaatacaccg
    41941 ataccggcgg cgaaaaacca ggaaacacca ataccaatca gaacaaatcg cgggcgggaa
    42001 atttcgcgtg cacaaaaaat cacgattaaa gccaccaaca agccaccagc aagaccagcc
    42061 actggacgcc agaacattcc cagcattgga aactggaaaa tcagccacag caccgccacg
    42121 ctacatcctt ctttgacgcc gattaaacca ggatcggcaa ggccgttacg cgcaatggat
    42181 tgcatcgcgg cccccgccat acctaacatg gctccgcata gcaatgccat gatgactctc
    42241 ggcaagcgaa tatcctgcac aatataacgt gcgtctgtgc ttacgttttc tggagcaaac
    42301 aacgctcgtc ctacttccga tgccgggaca aaaaaagaac catgcatcag accgaatatc
    42361 agtattccaa ccgcaaacag aaacagccca ccaagcaaca ccagattttt agggcgtacc
    42421 agtgtcgaaa acttaccaac acgtagtgga cgtagcccga cgttcattat ccgactcatt
    42481 tgaacattct cgtcgccata atgacaaaaa caggcgcacc aactaacgcc gtcatgacac
    42541 cggtcgctaa ctcatggggc gtgaaaagcg tccgagcaat aatatctgcc agtaataaca
    42601 aaagcgcgcc gacacaagct gaaagaggga gcagaacgcg caaatccgct gataccagac
    42661 gcctgacaat ctgcggtact agcagaccga caaaaccaat ggggccagca atcgatacgg
    42721 ctgcgccaca gagcaacgcg atcgcaagca gagtgaatgt tcgcgtgcgg agtaccgaga
    42781 cgcccagccc ctgcgccatg cgatcaccca gtgccaacat gttaagagag ggcgcaaggt
    42841 aaatggctaa cacaaccccc cccagagaaa accaggcgga cgtacccagc gtcgcccaat
    42901 cctgccccgc taaatcacct gctagccatg tccgcatcgc cagcagtgtt tgttcgtcaa
    42961 gaatcaggat tgcagccgtt attgaggata cgaacgccga tagcgccacg ccgcataaag
    43021 taactttcat tggcgttagc cctgaacgcc ctgcggagga aagcagcaat atcaacagga
    43081 acaataacgc gccgcccgta gatgccagca gtgggcgtcc aaccggaaag gccagcccaa
    43141 gcgcactggc agcaacgaca gcaagagcgg ctccggcatt taagcctaaa atatgtggct
    43201 cccccaatgg gttacgaatg acagcctgca acaaagcacc agcaactcca agagaagcac
    43261 cggtaagcaa tgctgccgct aaacgcggca aacggagtct tacaattacg ttgtgatcga
    43321 agttttgcgg ttcaaaatga agaaatgcct gcaacaccgt ttgtggtgca ataaatcggg
    43381 caccaatccc tggatatacc acgcatccca caagcagtaa ggcgaggaaa attacagtaa
    43441 ccagccctgt gcgcatcata ctttttctac accacgaaac ggcatgaaga acggctttcc
    43501 cgtcagagta tttattgatg cgtgaacatc aacgtcaaag acagctttta ccagctcagg
    43561 cgtgcaatgc tccccctcat tcaacacacg caccactttc ccctggcgta aaaatatcaa
    43621 cgtatcacca taattgacgg caaaattcag atcatgcaac accacgacga ctgtacgccc
    43681 gtgatgacgc gttaaatcat gtaataactc aagaatttca acctgataac gtaaatcaag
    43741 ccaggttgtg ggttcatcca gtaggatata aggcgtcttt tgcgccaaca ccatcgcaat
    43801 ccagcaacgc tggcgctgcc caccagagag tttttcaaca ggtaaatgag caaactcctg
    43861 cgtaccggtt aatttcaggg cctcttctac cgcctgctcg tcggcgtcgc tccattgacg
    43921 tatgaaattt tgccagggaa aacgcccacg cgacaccagt tcataaacgg ttaagccctc
    43981 cggcaataat ggcgactgcg gcaagatccc aagtctacgg gataacgctt tcgttggttg
    44041 ctcgtgaatc gctttaccat ccagcaatat tgagccgccc atgggttgca agacacgtgc
    44101 aatggtgctg agcaacgtcg actttccaca gccgtttgcc ccaaccagaa cggtcatttt
    44161 ttctgtggga acggagaaag aaacaccatc aacaatgatt ttcttgtgat acccggcaga
    44221 aacattgtcg aggatcagcc cctgcccttt tgcgttatgt gccacgtgta cgccaactta
    44281 atcaaagaaa aacaaaaata aaactcattc tcttttgaga gtttgacgcc acaaaatgta
    44341 gtagtcaaga agttaaaagc catacaaaaa caaggt
//
LOCUS       DAZFTN010000023        43466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFTN010000023 DAZFTN010000000
VERSION     DAZFTN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            178..1395
                     /gene="alaA"
                     /locus_tag="ACHKV2_001480"
     CDS             178..1395
                     /gene="alaA"
                     /locus_tag="ACHKV2_001480"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO6939393.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1479..2078
                     /gene="yfbR"
                     /locus_tag="ACHKV2_001481"
     CDS             1479..2078
                     /gene="yfbR"
                     /locus_tag="ACHKV2_001481"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6939394.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2137..3969)
                     /locus_tag="ACHKV2_001482"
     CDS             complement(2137..3969)
                     /locus_tag="ACHKV2_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6939395.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4056..4706)
                     /gene="hxpA"
                     /locus_tag="ACHKV2_001483"
     CDS             complement(4056..4706)
                     /gene="hxpA"
                     /locus_tag="ACHKV2_001483"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO6939396.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4717..5211)
                     /locus_tag="ACHKV2_001484"
     CDS             complement(4717..5211)
                     /locus_tag="ACHKV2_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6939397.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5294..5749)
                     /gene="yfbV"
                     /locus_tag="ACHKV2_001485"
     CDS             complement(5294..5749)
                     /gene="yfbV"
                     /locus_tag="ACHKV2_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO6939398.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6087..7289
                     /gene="ackA"
                     /locus_tag="ACHKV2_001486"
     CDS             6087..7289
                     /gene="ackA"
                     /locus_tag="ACHKV2_001486"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO6939399.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7364..9508
                     /gene="pta"
                     /locus_tag="ACHKV2_001487"
     CDS             7364..9508
                     /gene="pta"
                     /locus_tag="ACHKV2_001487"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6939400.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9698..11218
                     /gene="yfcC"
                     /locus_tag="ACHKV2_001488"
     CDS             9698..11218
                     /gene="yfcC"
                     /locus_tag="ACHKV2_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO6939401.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11251..11793)
                     /gene="yfcD"
                     /locus_tag="ACHKV2_001489"
     CDS             complement(11251..11793)
                     /gene="yfcD"
                     /locus_tag="ACHKV2_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO6939402.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11851..12402)
                     /gene="yfcE"
                     /locus_tag="ACHKV2_001490"
     CDS             complement(11851..12402)
                     /gene="yfcE"
                     /locus_tag="ACHKV2_001490"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO6939403.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12458..13102)
                     /gene="yfcF"
                     /locus_tag="ACHKV2_001491"
     CDS             complement(12458..13102)
                     /gene="yfcF"
                     /locus_tag="ACHKV2_001491"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO6939404.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13238..13885
                     /gene="yfcG"
                     /locus_tag="ACHKV2_001492"
     CDS             13238..13885
                     /gene="yfcG"
                     /locus_tag="ACHKV2_001492"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO6939405.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13942..14304
                     /gene="folX"
                     /locus_tag="ACHKV2_001493"
     CDS             13942..14304
                     /gene="folX"
                     /locus_tag="ACHKV2_001493"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO6939406.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14325..15218
                     /locus_tag="ACHKV2_001494"
     CDS             14325..15218
                     /locus_tag="ACHKV2_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO6939407.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15266..16156)
                     /gene="rpnB"
                     /locus_tag="ACHKV2_001495"
     CDS             complement(15266..16156)
                     /gene="rpnB"
                     /locus_tag="ACHKV2_001495"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO6939408.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16353..17126)
                     /gene="hisP"
                     /locus_tag="ACHKV2_001496"
     CDS             complement(16353..17126)
                     /gene="hisP"
                     /locus_tag="ACHKV2_001496"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO6939409.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17134..17850)
                     /locus_tag="ACHKV2_001497"
     CDS             complement(17134..17850)
                     /locus_tag="ACHKV2_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6939410.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17847..18533)
                     /gene="hisQ"
                     /locus_tag="ACHKV2_001498"
     CDS             complement(17847..18533)
                     /gene="hisQ"
                     /locus_tag="ACHKV2_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO6939411.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18623..19405)
                     /gene="hisJ"
                     /locus_tag="ACHKV2_001499"
     CDS             complement(18623..19405)
                     /gene="hisJ"
                     /locus_tag="ACHKV2_001499"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO6939412.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19626..20408)
                     /gene="argT"
                     /locus_tag="ACHKV2_001500"
     CDS             complement(19626..20408)
                     /gene="argT"
                     /locus_tag="ACHKV2_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO6939413.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20674..21243)
                     /gene="ubiX"
                     /locus_tag="ACHKV2_001501"
     CDS             complement(20674..21243)
                     /gene="ubiX"
                     /locus_tag="ACHKV2_001501"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO6939414.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21338..22855)
                     /gene="purF"
                     /locus_tag="ACHKV2_001502"
     CDS             complement(21338..22855)
                     /gene="purF"
                     /locus_tag="ACHKV2_001502"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO6939415.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22892..23380)
                     /gene="cvpA"
                     /locus_tag="ACHKV2_001503"
     CDS             complement(22892..23380)
                     /gene="cvpA"
                     /locus_tag="ACHKV2_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO6939416.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23912..24574)
                     /gene="dedD"
                     /locus_tag="ACHKV2_001504"
     CDS             complement(23912..24574)
                     /gene="dedD"
                     /locus_tag="ACHKV2_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO6939417.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24564..25832)
                     /gene="folC"
                     /locus_tag="ACHKV2_001505"
     CDS             complement(24564..25832)
                     /gene="folC"
                     /locus_tag="ACHKV2_001505"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO6939418.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25902..26816)
                     /gene="accD"
                     /locus_tag="ACHKV2_001506"
     CDS             complement(25902..26816)
                     /gene="accD"
                     /locus_tag="ACHKV2_001506"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO6939419.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26972..27631)
                     /locus_tag="ACHKV2_001507"
     CDS             complement(26972..27631)
                     /locus_tag="ACHKV2_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6939420.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27714..28526)
                     /gene="truA"
                     /locus_tag="ACHKV2_001508"
     CDS             complement(27714..28526)
                     /gene="truA"
                     /locus_tag="ACHKV2_001508"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO6939421.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28526..29539)
                     /locus_tag="ACHKV2_001509"
     CDS             complement(28526..29539)
                     /locus_tag="ACHKV2_001509"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6939422.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29605..30741)
                     /gene="pdxB"
                     /locus_tag="ACHKV2_001510"
     CDS             complement(29605..30741)
                     /gene="pdxB"
                     /locus_tag="ACHKV2_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO6939423.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30840..31835
                     /gene="flk"
                     /locus_tag="ACHKV2_001511"
     CDS             30840..31835
                     /gene="flk"
                     /locus_tag="ACHKV2_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO6939424.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31832..33010)
                     /locus_tag="ACHKV2_001512"
     CDS             complement(31832..33010)
                     /locus_tag="ACHKV2_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939425.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33285..34505)
                     /gene="fabB"
                     /locus_tag="ACHKV2_001513"
     CDS             complement(33285..34505)
                     /gene="fabB"
                     /locus_tag="ACHKV2_001513"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO6939426.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34664..36670
                     /gene="mnmC"
                     /locus_tag="ACHKV2_001514"
     CDS             34664..36670
                     /gene="mnmC"
                     /locus_tag="ACHKV2_001514"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO6939427.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36791..37069)
                     /locus_tag="ACHKV2_001515"
     CDS             complement(36791..37069)
                     /locus_tag="ACHKV2_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO6939428.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37103..37651)
                     /gene="epmC"
                     /locus_tag="ACHKV2_001516"
     CDS             complement(37103..37651)
                     /gene="epmC"
                     /locus_tag="ACHKV2_001516"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO6939429.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37651..38460)
                     /locus_tag="ACHKV2_001517"
     CDS             complement(37651..38460)
                     /locus_tag="ACHKV2_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6939430.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38460..39284)
                     /gene="mepA"
                     /locus_tag="ACHKV2_001518"
     CDS             complement(38460..39284)
                     /gene="mepA"
                     /locus_tag="ACHKV2_001518"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO6939431.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39288..40373)
                     /gene="aroC"
                     /locus_tag="ACHKV2_001519"
     CDS             complement(39288..40373)
                     /gene="aroC"
                     /locus_tag="ACHKV2_001519"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO6939432.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40408..41340)
                     /gene="prmB"
                     /locus_tag="ACHKV2_001520"
     CDS             complement(40408..41340)
                     /gene="prmB"
                     /locus_tag="ACHKV2_001520"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6939433.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41506..42057
                     /gene="smrB"
                     /locus_tag="ACHKV2_001521"
     CDS             41506..42057
                     /gene="smrB"
                     /locus_tag="ACHKV2_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO6939434.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42228..43070)
                     /locus_tag="ACHKV2_001522"
     CDS             complement(42228..43070)
                     /locus_tag="ACHKV2_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO6939435.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43072..>43466)
                     /locus_tag="ACHKV2_001523"
     CDS             complement(43072..>43466)
                     /locus_tag="ACHKV2_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6939436.1"
                     /translation="NLLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKV
                     TLMGTEDTDLPGFLSIDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNF
                     NAWLQTVNGRNVTSGDFTATMTVTFEYF"
BASE COUNT        10745 a        11740 c        10853 g        10128 t
ORIGIN      
        1 aaagcatatg cacctcatta ttttgtcatt attaagttat taacagcaca atcgagcctt
       61 cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg
      121 ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg
      181 tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg
      241 ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc
      301 aacccagccc cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac
      361 ctgcctaccg ctcaagggta ttgcgattcc aaaggtcttt actccgcgcg taaagccatc
      421 atgcagcact atcaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc
      481 aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa
      541 atgttggttc ctgcaccaga ttacccacta tggaccgcgg cggtttcgct ttccagcggt
      601 aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt
      661 cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa taacccaacc
      721 ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat
      781 ctcattatct tcgccgatga aatttatgac aaaattctct acgacgacgc tgagcatcac
      841 tcaattgcgc cgctggcacc tgacctgctg accattacct ttaatggact gtcgaaaacg
      901 taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc
      961 aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct
     1021 gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc
     1081 cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aattgatcaa cgatattccg
     1141 ggcgtttcct gcgtgaaacc ttgtggtgcg ctgtacatgt tcccgaaaat cgacgccaaa
     1201 cgctttaaca ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt
     1261 ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg
     1321 ctaccgcgtg tcgatgatat cgagctgtcg ttgagcaagt tcgcgcgttt cctttctggt
     1381 tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt gttatcccgc
     1441 cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg
     1501 cccatctctc ccgcctgaaa ctcattaacc gctggccgct catgcgcaac gtgcggacgg
     1561 aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca
     1621 aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc
     1681 acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc
     1741 aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg
     1801 ttccggaaga gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg
     1861 aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag
     1921 aactcgcggc gggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg
     1981 aagcgcgccg cagccaggag atggactact tcatggaagt atttgttccc agcttccatc
     2041 tttcgctcga tgagattagc caggattcac cgctgtaagc agctggagtc tgcgtcgcat
     2101 caggcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat
     2161 catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccagct tcacaaagtc
     2221 actgaagctg taatttcccg gacctaaaac caatgtgtta acaggtgaag aaaccggtgt
     2281 cataaaggcg gcggatgctg ccatcgccac gaccatcgcg aatggataag gcgacacccc
     2341 catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga
     2401 gataaatagc ccaataaccg ccgacaagac aaacaaacaa cccagcatca tatgtggccc
     2461 gtaaccaccg ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg
     2521 taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga
     2581 tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat
     2641 agcggcaaca ggattaggaa tttcatctgt cagcattaac gccaccatta gcaccagaca
     2701 gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa
     2761 gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc
     2821 cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc
     2881 cacgccattg cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc
     2941 gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac
     3001 atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc
     3061 actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg
     3121 cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg
     3181 ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg
     3241 accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata
     3301 ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt
     3361 atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac
     3421 caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc
     3481 actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc
     3541 ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct
     3601 taacaccacg ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc
     3661 cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg caactttgac
     3721 cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa
     3781 caaggcggca atcaggacaa cgttaggatc agaaaagcca gaaaatactt ctgggactgt
     3841 cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac
     3901 tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc
     3961 accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc
     4021 cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt
     4081 ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg
     4141 cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa
     4201 agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc
     4261 tgcgcgccta acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt
     4321 acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc taccggcatg
     4381 gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg
     4441 attgcccccg gaagcgcggt aataccttcg gtttccgtgg cctcgatttg ctccaaacgc
     4501 gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg cagagaggtg
     4561 atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg
     4621 gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga
     4681 tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat
     4741 ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg
     4801 atatttttcc cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt
     4861 atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc
     4921 ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt
     4981 agaccaggaa acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt
     5041 ttcttctttc agctcgccaa actcgcgatc cagttcacgc atctgtaatc cataaccccg
     5101 ctcaatgatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat
     5161 catcttgtac tggttagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc
     5221 ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt
     5281 aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcacgct
     5341 taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc
     5401 cagactcctg caatttaccg cgaacttcat aaaaccagtt gaggattgca gggggtaatg
     5461 gcgtgacaga acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca
     5521 gggcagtggc aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa
     5581 atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt
     5641 cgacaaagac cggggcaagg cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc
     5701 ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca
     5761 acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca
     5821 aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta
     5881 gtacatattc attgagtcgt caaattcata tacattatgc cattggctga aaattacgca
     5941 aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg
     6001 ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta
     6061 gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg
     6121 gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg
     6181 gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata
     6241 aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta
     6301 atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta
     6361 tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg
     6421 gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg
     6481 aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg
     6541 cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca
     6601 aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg
     6661 aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg
     6721 gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc
     6781 tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca
     6841 tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca
     6901 aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca
     6961 actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga
     7021 aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcaccg
     7081 gtggtatcgg tgaaaatgcc gcgatggtcc gtgaactgtc tctgggcaaa ctgggcgtgc
     7141 tgggctttga agttgatcat gaacgcaatc tggctgcacg tttcggcaaa tctggtttca
     7201 tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg
     7261 cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg
     7321 ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat
     7381 gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc
     7441 aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg
     7501 tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc
     7561 cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt
     7621 gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct
     7681 ggttgaaggt ctggtcccga cacgtaagca ccaatttgcc cagtctctga actacgaaat
     7741 cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga
     7801 acagctgaaa gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa
     7861 catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg
     7921 cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc
     7981 ggcgaagctg caagaatcca gcccgctgcc ggttctcggc gctgtgccgt ggagctttga
     8041 cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga
     8101 aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca
     8161 catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt
     8221 gctggttgcc gcttgcctgg ctgccatgaa cggcgtagaa atcggtgccc tgctgctgac
     8281 tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg
     8341 cctgccggta tttatggtga acaccaacac ctggcagact tctctgagcc tgcagagctt
     8401 caacctggaa gttccagttg acgatcacga gcgtatcgag aaagttcagg aatacgttgc
     8461 taactacatc aacgctgact ggatcgattc tctgactgcc acttctgagc gcagccgtcg
     8521 tctgtctccg ccagcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg
     8581 tatcgtactg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga
     8641 acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc
     8701 gtctcagggt gtagaactgg gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga
     8761 aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc
     8821 ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt
     8881 tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca
     8941 gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc
     9001 ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg cagaacagct
     9061 ggcagaaatc gcgattcagt ccgctgattc cgctgcagcc ttcggtatcg aaccgcgcgt
     9121 tgctatgctc tcctactcta ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg
     9181 cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca
     9241 gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc
     9301 aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc
     9361 ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc
     9421 ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac
     9481 tgcgattcag tctgcacagc agcagtaacc tcgtcatcat ccgcagcttt gcgctgcgga
     9541 tatctgaacc ggaaatagtc actatttccg gttttttatt ctcttaattt gcattaatcc
     9601 tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata
     9661 cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa
     9721 accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt
     9781 aaccagcctt gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta
     9841 tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgccttct
     9901 gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga
     9961 acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga aatacgggac
    10021 agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg
    10081 tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgtg ggaatgaaat
    10141 tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg
    10201 ggaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga
    10261 cagtattacc accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg
    10321 gatgaacccg ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg
    10381 ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat
    10441 ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg
    10501 cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct
    10561 ggtattgatt gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc
    10621 ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat
    10681 cggcgttgtt ttccgcctta acggtatgac ggttaatacc atggcttcat ccttcaccga
    10741 aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct
    10801 gctggtcggt aatggtgaag cgggtgatgc cagcgtgtta aatactatcc tcaacagcat
    10861 tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca
    10921 ggcggtattt aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt
    10981 actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggctttcca
    11041 gtttggtgat ggcttcagtc acatcattta cccgacctca gcttcgttaa tggcgacgct
    11101 cggtgtttgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact
    11161 gctgtttatt atgtccagcg tcgtggtgat cggcgctcag ttgatgggct accactaaaa
    11221 atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc
    11281 atttttggca ttgcgcttca tccacaacgc tagcgctttc agcgagtctg gagtgaactc
    11341 atcgcagcgt gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc
    11401 ctgtagggcg aagggaccgt gagagacgca gctgaacaat gcgccccaga cacggcaatt
    11461 tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc
    11521 ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgga ctacaccgcc
    11581 agcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag
    11641 aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc
    11701 ccgcatttgc tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac
    11761 ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc
    11821 tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat
    11881 actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc
    11941 cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg
    12001 ctgttctgct actggcagat gggtatgacc gtaaaccagc acatcgttct ggtttaaagc
    12061 aggcagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag
    12121 taatacctgt tgccacggcg cggttatcgg gaaatgcagc agcatttgat ccacttcgct
    12181 gtcgcagttg ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac
    12241 tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc
    12301 aaggatcacc agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc
    12361 cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa
    12421 aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc ttcgctgaaa
    12481 gtgcaataaa acgctggaca gacgctcgct gccactggaa tgttgcataa tccaccaggc
    12541 attctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat
    12601 cagcaatgca ccattcacca aataaattcg gctgacccag tactaacaaa tgttctgcca
    12661 tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc
    12721 ccgcaaagac aacatctgtc ggacgctctt cgcggatggg catcagatcg ctgcgcaacc
    12781 aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc
    12841 aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact
    12901 caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg
    12961 gttgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacaac aggccttttt
    13021 cctgcaacgc cacccaagcg gataacacat aaggggagaa aaaatgggca tctgaccaaa
    13081 gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta
    13141 tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata
    13201 attgtggtgt tcagatgatt agacaaccgg agctccaatg atcgatctct atttcgcccc
    13261 gacacccaat ggccacaaaa ttacgctgtt tctcgaagaa gcagggctgg attatcgctt
    13321 gataaaggta gacctgggga aaggcggtca gtttcgcccg gaatttttgc tcatttcgcc
    13381 taacaacaaa attccggcaa ttgttgatca ttctccagcc gatggcggcg aaccgctaag
    13441 cctctttgaa tctggtgcca ttttgttgta tctggctgag aaaacggggc tctttttgag
    13501 tcatgaaacg cgtgaacgcg ccgccacatt acagtggtta ttctggcagg taggcggact
    13561 ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta
    13621 cgctattgaa cgttatcagg ttgaaactca gcgtctgtac catgtactga acaagcggct
    13681 ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc
    13741 gtgggttaat gcctggactc gccagcgaat agacctcgct atgtatccgg cagtcaagaa
    13801 ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact
    13861 cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag
    13921 taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt
    13981 cgtttacgta catttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt
    14041 atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat
    14101 gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc
    14161 tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg
    14221 acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc
    14281 atgaccttaa gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca
    14341 ccggagggac gggattaatt ggtcgccatt tgattccacg tttgctggag ctgggccatc
    14401 aaatcacggt aatgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga
    14461 cactatggca agggcttgcc gatcaaagca acctcaacgg cattgatgcg gtaatcaacc
    14521 tggccggaga accgattgct gataaacgtt ggactcacga gcaaaaagag cgtctctgcc
    14581 aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccac
    14641 cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg
    14701 ttaccgaaga ggaaccgccg cataacaaat ttacccataa actctgcgcc cgctgggaag
    14761 aaattgcctg tcgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcgt accggcgtag
    14821 tgctggcacc ggatggcggt attctcggta aaatgctgcc gccgtttcgt cttggcctgg
    14881 gcgggccgat tggttccggt cggcagtatc tggcctggat tcatatcgat gatatggtca
    14941 acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct
    15001 acccgatacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta
    15061 ttttgcgcgt ccccgcaacc gccattcggc tgttaatggg cgaatcttca gtactggtat
    15121 taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt
    15181 acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttactg caaacatccg
    15241 ccagttaact cccggtgtta cagggttagt ggctttgcgc gataagatcg tctggtgaaa
    15301 gtcgggtcag cgtcaggatg acttcgtggt caaatccttt ttccagcatc tcctgagcaa
    15361 tacgcagagc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa
    15421 tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac
    15481 ggaaacgctg ggcatctccc gtttgtaata cataattaaa cagggctttt agctgtctgt
    15541 cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc
    15601 gctgacgaat atgtttctga attaactcca acagcgccat tttacgatgt tgcataatct
    15661 cgtcatccgg caccacggta atatccacca acggaaaagc cgatgaatat attttacggg
    15721 caatcgcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc
    15781 aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc acatcaagat
    15841 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
    15901 tactttggtg ctctatcact acataaagat aacccactcc ctcctgcgtt ttcacagacc
    15961 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt
    16021 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
    16081 cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
    16141 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
    16201 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
    16261 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa
    16321 tactccggca gcgttagcca ccggagaggc aattatttca gcgaaccttt aaggaactgt
    16381 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccttc ttcctctatt
    16441 ttcccctgat ggaggaaaat gacgtgagta gatacatggc gagcaaagcc catttcgtgg
    16501 gtcaccacca ccatcgtttt tccctcttcc gccagctgct gcataatgcg cagcacttca
    16561 cccaccagct ctgggtcaag ggccgatgtc ggttcatcaa acaacagaac ttccggttcc
    16621 attgccagcg cccgcgctat ggaaacacgc tgttgctgac caccggacag gtgcaccggg
    16681 tatttcccct gcgcacgttc gtctatcccg acttttgcca gatacttcac cgcccgctcg
    16741 cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc
    16801 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
    16861 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
    16921 atcgtctggc cattgaccac gatcgaccct tcactcggtt tttcgaggaa gttaatgcag
    16981 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
    17041 ttcgcttgca gtgacacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg
    17101 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
    17161 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
    17221 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
    17281 gcgggctatt ttcagcagat ccggcaccgt ggcagtaaat gccaacgcag tagagtgcag
    17341 catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
    17401 aatgcagcga tacattttaa aagtcgagaa gccataggct ctggcggctt caatttcccc
    17461 atgcggcacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
    17521 cgtcagcgcc agcacggtac agttcaggcc actgcggaaa aaggcgttaa gaaactcggt
    17581 ccccttaaca atctccagcg tatacatgcc ggaatagaac accagcaact gaacatacag
    17641 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
    17701 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
    17761 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
    17821 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacagagtag
    17881 cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg
    17941 ccacagacga tagcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct
    18001 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
    18061 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
    18121 aacacttgcc cacgagtaaa accgaacgcc ctcgccgcct ctatatgtcc tttcggcact
    18181 gccataaaag caccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
    18241 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
    18301 gtgtttagcg caatctgcaa accgtagaaa atcagcagca tcaacactaa gtccggcacg
    18361 ccacgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
    18421 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
    18481 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct
    18541 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
    18601 ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg
    18661 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc
    18721 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
    18781 ctttaacaga cgggccaccg aatttgtaat ctttaccgac aggttgtttg aggaaacctt
    18841 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
    18901 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc caatgttcat
    18961 taccgaacgt ctcctgggta gtgccctgca atacgcctac ccgtttgcct ttcagcgact
    19021 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca
    19081 gtttgtcggt gaaggctatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
    19141 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcgga ttttcgacaa
    19201 acgtacattg cgtattgatg cgtttgcata attcctttgc cagatcgata tcgaagccaa
    19261 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
    19321 ggatgttttg cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc agaaccagag
    19381 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
    19441 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
    19501 cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
    19561 tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag cttccctgcg
    19621 acgtatcagt caccgtagac attaaagtca aaatactttt tcgccatctt gtcgtaggtg
    19681 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
    19741 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
    19801 gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
    19861 tcctgcagtg cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg
    19921 gcataggcca ccacatccac acctttgcta cgccaggtct cgttagcgta agcttcctgg
    19981 gttgatccct gcagcacacc aacatgttta cctttcagtg agtccagcgt tggctgaatc
    20041 ggcgaacctt tggcggcaat cagacgggaa tctgcggcgt acagcttgtc ggagaaggca
    20101 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc gatttttttc
    20161 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc
    20221 atccgtttgc acatctcgtt accgagatcg atatcaaagc cgacaaagtc acctttggca
    20281 tctttcgacg agaacggtgc ataggtggta tcggttccga tacgtaccgt ctccggcaat
    20341 gccgcatagc tggaagccgc tgcggaaata ccgactaaca aagacagagc gagaatcgac
    20401 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
    20461 tttgcaggct tgttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
    20521 taaatatgtt aataagacgt tgcattattg tcctaaagtt gaagatagca ggtatagcgg
    20581 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
    20641 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
    20701 atcttcagga agggttatcg caaactggtc aagaacacga ttaaccgtct gatttatcac
    20761 atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc
    20821 gatttctgct gcctgcgtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
    20881 gcagagcacc agtgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
    20941 atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat
    21001 ccccagtgtc tggaaagaac cggaagagat gctggcggca atatcgcgcg catcatgcgt
    21061 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
    21121 gcgcgccgcc tggctcatta ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
    21181 taataagcgc acaccataaa tcgcgccgct ggcaccgctg atgcccacaa taagtcgttt
    21241 cataaatagt tgccctgtca gacttgcagg cagactttgc aggatttcgg cgggagttgc
    21301 aagtcagggt gccagaccgg caccctgagc gaaggcatca tccttcgtta tgcatttcga
    21361 gattttccac ttcgttctga cgttgcactg ctttggcgtc gtcattacgt aaagtatcga
    21421 ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
    21481 attcaaactg ctggatatcc gggttttcag cgcgaacggc ttcgatcaga tcgttcagat
    21541 cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatctact tcgcgaccgt
    21601 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
    21661 ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
    21721 tctgctcaga agtggtgcca cggacgatag agtcgtcgac cagcaggacg tttttatcgc
    21781 ggaactcggc gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctgac
    21841 ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt
    21901 tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttccggg atcgggatca
    21961 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagc ttcgtgccca
    22021 tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgagcaaagt
    22081 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
    22141 actgcccttc ttcagtgatg taaatcgctt cgccaggcgc gacgtcacgc aggaaatcaa
    22201 agcccagtgt atcgagcgca acgctttcgg aagcgaccat atattctgtg cggttctcgt
    22261 caatatcacg ttttcccaga accagcggac gaataccgtt tggatcgcgg aaagcaacca
    22321 taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtag
    22381 cagcgatggc ggcgaaaatg ttgtcggctt caagcgggta gtggcggaag ttgtccagct
    22441 cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
    22501 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
    22561 gcgtaatgcc atacggagag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
    22621 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
    22681 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
    22741 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
    22801 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
    22861 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
    22921 tttgcagata atcaaaaaac catctgatga taaaactgaa ctgcggaatc agttgtgatt
    22981 tactccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatagcag
    23041 caacaatcaa cacaccgcgc aatgcaccga aacagacgcc cagcacccga tcggtgcctg
    23101 acaaccccgt tttctccacc agcaggccta tcacgaagtt cacgatagcg ccaacgatca
    23161 gggtggcgat aaacaggacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc
    23221 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
    23281 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
    23341 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
    23401 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
    23461 ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
    23521 gcctttgcgg catcggacaa tgcgtgtcgg atgcggcgta aacgtcttat ccgacctacg
    23581 gttctacccc tgcgtaggcc tgataagacg caccagcgtc gcatcaggca acggctgtcg
    23641 gatgcggcgt gaacgcctta tccgacctac ggttctaccc ctgcgtaggc ctgataagac
    23701 gcaccagcgt cgcatcaggc aacgcctgtc ggatgcggcg tgaatgcctt atccgaccta
    23761 cggttctacc cctgcgtagg cctgataaga cgcgccagcg tcgcatcagg caacggctgt
    23821 cggatgcggc gtaaacgcct tatccgacct acggttctac ccctgcgtag gcctgataag
    23881 acgcgccagc gtcgcatcag gcaagaccgt attaattcgg cgtatagccc attaccacgc
    23941 cacttaagcc agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg
    24001 catccggccc aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat
    24061 aaacccgata accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt
    24121 tcagcgcgcc cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga
    24181 ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac cttcggctta ggcggctcca
    24241 ccggtttcgg cttcggtggg acgaccggtg caggttccgg ttcaaactcg gtgttattgg
    24301 ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg
    24361 ctgcgccttc cggcggttgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct
    24421 catcgcgatc gcccgctttt ggcaccagcg ggatagccgc aaactcatcc tgataatgtt
    24481 ttttctgccc gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg
    24541 tgcccactaa ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc
    24601 atgacatgtg cgaccgtgtg gaacgaacca cacaccagca cggtgtcttc cgctttagcg
    24661 tccgccattg cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa
    24721 tgctcaagca gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag
    24781 tcatcaacca cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata
    24841 ccgataaccg ccagcacgcg cccgtttttc ggtagcgttt tcatacgccc ggtgagatat
    24901 tccgccgcat gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc
    24961 tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgacc
    25021 tccagcccgc tggcacgcag tgccgccagc gccgttgcgg cattcggttg cgggacaagc
    25081 ggcaacggca ggttttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg
    25141 acggaatagt tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca
    25201 tcagcaatgg tagaaggcat ttccggctca ccgacaattg ccggtttttc gctgcgaaag
    25261 atgcctgctt tctcgcgacc aatactttcg cgatctggcc ccagccagtc ggtatgatcc
    25321 agcgcaatac tggttactac cgcgacatcg gcgtcgacaa tattggttgc gtccagacga
    25381 ccgcccagcc ctacttccag aatcaccacg tcaagttgtg cctgtttgaa cagccacaac
    25441 gccgacagcg taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc
    25501 tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct gaccctgcac acgtacgcgc
    25561 tcggtataac acaccagatg aggcgaactg tagacgccca ctttgtaccc tgccgccatc
    25621 agaatcgact ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac
    25681 acaaatggcg ctggtttcag aacgccaaga cgcgccgcga cctggctcac gcgctcaagg
    25741 ccgagatcga tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc
    25801 gacgcggctt gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa
    25861 tagcaaaagg gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga
    25921 tccggtaccg ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga
    25981 ttcatcaact tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc
    26041 atgtcgatcg cgcctttctc gatcaggaat tcactgcgct ggaatcctgg cggcagtttt
    26101 tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttcggttca
    26161 gcgatgttga gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg
    26221 gtcagcacgg agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt
    26281 ttcgccatct gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag
    26341 aagcagatca gcgggcagtt atcttccagc gcctgctcaa cggcacgcac gaaacgtgca
    26401 cccacaacag accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc
    26461 ggcattccat acagagtgcc tttcatcacc accagcgcat ctttttcgcc ggtttctttc
    26521 tgcgcagatg ccagacggtc tttatacttc ttggagtcac gaaacttcag cacatctttc
    26581 ggctcaagct cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga
    26641 ttacgcgctg tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc
    26701 agctcagcgc ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga
    26761 atgctcgcct tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg
    26821 gacctttctg tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat
    26881 ttttgccccc aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga
    26941 taaaaaagtg gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt
    27001 tgtgacggat tatttcgatg acgcccggca aaatagaaac cacaataatc ccgacgatca
    27061 gcagcttaag gttatcctga accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa
    27121 aaagcagtac ccacaacagt gcgccgatca cgttataagc agcgaaatga cggtacgaca
    27181 tgtggcccat tcccgcaaca aacggggcga acgttctgac gatcggcaca aaacgggcga
    27241 gaataatcgt tttgccgcca tgtttctcat aaaactgatg ggttttatcg agataactgc
    27301 gacggaaaat tttcgaattg ggattactga ataacttttc accgaacaag cgcccgatag
    27361 tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat cagcaccacc atcatatgga
    27421 cgttgagatc gttggtttcc agtgatgcca acgctccggc aacaaacagc aatgaatcac
    27481 ccggtaaaaa cggcgttacc accagaccgg tttcacagaa caaaattaag aacaaaatgg
    27541 cataaaccca gacgccgtac tccgcgacca gctctgccag atgcacgtca atgtgcagga
    27601 taaaatcgat gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt
    27661 tcaggctgta actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc
    27721 gccagaaata gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc
    27781 gcgaccagat acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc
    27841 gccagcaact ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg
    27901 ccgacaatat tcctgaccat atgatgtaca aaggcattcg ctttgatatc aaccaccaca
    27961 taaggaccgt gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg
    28021 cactgcaccg cacggaacga ggtgaaatca ttctcgccca gcaagcactg cgcagcccga
    28081 tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc
    28141 gggcgcagcc gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg
    28201 gcatgaaaat catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc
    28261 gcatttacgc ccaacgtcca cgcagcgtct ttgcgctgcg cggtagtttc gaaatgcaca
    28321 acctgcccgg taccgtgtac ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt
    28381 tcgttcgcca cctgggagag cgccttttcc agcttctcct gcacactgcg gacttcattc
    28441 tgccgttgcc agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa
    28501 actggcggtt gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga
    28561 tttttactgc catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagaactgga
    28621 cttgctccgg cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg
    28681 aagcatcacc tacctgagtt gggaattcgt tctcttcaga gagcacaata tcttcccctt
    28741 gaacaaacgc atcacgcgct tcttctgctg ccagtggacg cagagcttca aagttgacca
    28801 tctgggcatg accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagtcctt
    28861 cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt
    28921 cgctatccgg cagtaacggc agcatgttga acgcgagctg acgcccgaag aaatcttctt
    28981 cgtcaatcgg aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt
    29041 ttttgccctg ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct
    29101 gatcgattaa cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga
    29161 tgacattccg gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg
    29221 gtacgtcggg ttcaagagca aacaatccgc tgctgtcgat caccaggcaa cctgagttgg
    29281 tcgcttcttc aacccaggct gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg
    29341 cctgcgtcca gtcgaattca gcagcatcct gcacggtgat tgtcttacca ccaaagcgca
    29401 attgttcgcc tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac
    29461 gttcagccag cgtttcaagc agggcttcgc ccacagcgcc agttgtgccc aggacggcaa
    29521 tgttccagcc ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt
    29581 atagcagaga gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag
    29641 tttacacagc aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc
    29701 acggcgctca agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt
    29761 acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt
    29821 cggttgatcg agcggtccat gcagagtaat gcgaccaaac tctggcgcag gcagtaatgt
    29881 atccagcgca acgtgctgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt
    29941 agtaccgcgt gctttacctt ccagggtata gcctgcaata tgcggcgtgc cgatatccac
    30001 ttttgtcagc aattccacat ttagttccgg ttcgccttcc cagacatcca gcactacgct
    30061 taacttctgg ccttcattca ggcaggtcag cagcgcggta ttatcgacga ctgcgccacg
    30121 gcaggcgtta atcagaatcg ctccgggctt caggctacgg atcagttttt catccgccag
    30181 atgcagcgtt ttgtacggcc cgtctttgaa gagtggcgta tggaaagtca gaatatccgc
    30241 gcgctggact aactcatcca gcgagcggaa atcaccctca tccccacagt cggcgcgagg
    30301 cggatcgcaa agtaaggttt taatccctaa cgcttccagt cgtgcctgta aacgccgacc
    30361 aacgttacca acgcctacga tccccacggt acgctcgtgc agtgaaaatc catcgcgttc
    30421 ggcaagcatc agcagggagg agaaaacata ttccaccacc gcaatcgcat tacagccagg
    30481 tgcagcggaa aaaccaattc ccgcctgctt caaccatgct tcatcgacat ggtcggtccc
    30541 cgcagtggca gtgccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt
    30601 cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg
    30661 cccgggaacc gcgatcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt
    30721 ttcatcaaca aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa
    30781 gtgtgccata atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa
    30841 tgatacaacc tatttccggc cctcctcctg ggcaaccacc aggtcaggga gataacctgc
    30901 cgtctggcgc gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa
    30961 tgacgaaagt gacctcactg acgcaacagc aaagagcaga actgtgggcg ggtatcaggc
    31021 acgatattgg tctgtcggga gattcaccgc tgctttcgcg tcactgccct gccgctgagc
    31081 ataatctggc gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag
    31141 cgcaattagg ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc
    31201 gtcataactt tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc
    31261 tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg
    31321 atcgtcctct attaccggcg gagcacaatg cgctcaaaca gctggtgacc aaacttgcgg
    31381 cggcaacggg ggagcccagc aaacagatct ggcaatcgat gctggaactt tccggggtga
    31441 aagatggcga gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ctgcaggcgc
    31501 gtcagacgct aagccagcaa aatacgccga cgctggaatc actacaaatg acgctaaaac
    31561 aacctttaga tgccagtgaa ctggcggcgt tatcggcata tatccagcaa aaatatggcc
    31621 tttctgcgca atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc
    31681 aacggcgggt taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac
    31741 cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg
    31801 cgattatcct gatgctggtc tggctggttc gttaaccccg acgaaacgac agtatcgtga
    31861 caataattcc cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg
    31921 tggtcgccag cattcccgca agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg
    31981 cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacgc
    32041 ccagcgcagg aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga
    32101 cccacgcccc tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga
    32161 caatcgccac tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca
    32221 cgacaaatgc gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg
    32281 caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagac
    32341 ctaaccctgg tttccagata agtccgacaa cgctccacag agatggacgt tctcccgcca
    32401 gggctggtac tttgcgcact gtgccgttac aggcccacgc cagtaagggt aatgccattg
    32461 tggtgagcgc cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggagcac
    32521 caacagcgag ggcaccgtaa atcgccattc cattccatga catcactttg ccagagtgtt
    32581 ttggccctac gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa
    32641 acccaagaat caaacgcccg atgaccaaca gggcaaattt gaacggtgcg gagacaggca
    32701 aaatcgccgc cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg
    32761 ccgaacgttt tgcgccatat tgatcggcca gtcgcccggc atagccacgc gtcagcaccg
    32821 tagccagaaa ctgaatgccg acggcaatgc cgaccatggt attgccatag cccagatcat
    32881 gatgaacaaa caacgggata accggcaacg gcaaccctac ggtcatgtag gtgagaaaaa
    32941 ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta
    33001 cagcagtcat gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc
    33061 tctggtttca gggttacagt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa
    33121 atcgacaaag cggggagttt gcctggaact ggcggcgatt gtcaatgatg tgaaaaaggg
    33181 aaccatcagg ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg
    33241 cgacgctggc gcgtcttatc cgacctactc cggcctactg cgaattaatc tttcagcttg
    33301 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc
    33361 agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct
    33421 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    33481 tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc
    33541 gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga
    33601 gtcgaagtgc cgtgggagtt caggtaatcg attggggtat caacgccatg catcgccatc
    33661 tgcatacaac gtactgcgcc ttcgccagag ggagcaacca tgtctgcgcc atcagacgtt
    33721 gcgccgtagc caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc
    33781 tcttcaacca ccaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcatcg
    33841 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccattgcg
    33901 tcgaattcgc aagccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    33961 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    34021 gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag gcaggcagaa
    34081 acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    34141 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc agaacctgca
    34201 atcaggccaa cacgcggatt attctggtaa gcttccggag agaggcccgc atctgcgatt
    34261 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    34321 cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg
    34381 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    34441 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    34501 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    34561 cttgtacgcc gaacaagtcc gatcagccaa ttaatagaga aatttgcgca gccttacaca
    34621 catcgctaag atcgagctac cgcctgtaag acgagtaact tacgtgaaac actactccat
    34681 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    34741 tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa
    34801 ccaattagag gcacgctttc ctgaacatcc acatcctctg tttgtggtag cagagagcgg
    34861 cttcggcacc ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc
    34921 gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    34981 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    35041 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattac ttgatgaagg
    35101 ccgcgtgacg ctggatttat ggtttggcga tattaatgaa ctgatcagcc aactggacga
    35161 ttctctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag cgaaaaaccc
    35221 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac
    35281 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac
    35341 gatgcaaaaa cgtaagggct ttggacgcaa acgggaaatg ctttgtgggg tgatggaaca
    35401 gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga
    35461 agtagcgatt atcggcggtg gtattgccag cgcattgttg tcgctggcgc tattgcgtcg
    35521 cggctggcaa gtaacgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa
    35581 tcgccagggg gcgttgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    35641 ctctaatggg tttacttttg ctcgtcgact ttatgattca ttacccgtta aatttgatca
    35701 tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca
    35761 gatgttgtca atggatttac ccgaagaact ggctgtagcc gttgaggcaa atgcggttga
    35821 acaaattacg ggcgttacga caaattgcag cggtattact tatccgcaag gtggctggct
    35881 gtgtccggca gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat
    35941 ttattatcaa tatcagttac aggatttatc ccgtaaggat gactgttggt tgttgacgtt
    36001 tgcaggagat cagcaagcga cacacagtgt agtggtactg gcgaacgggc atcaaatcag
    36061 ccgtttcagc caaacatcgt ctctgccagt gtattcggtt gccggtcagg tcagccatat
    36121 tccgacgaca cccgaactgg caaaactgaa acaggtgctg tgctatgacg gttatctcac
    36181 gccgcaaaat ccggcgaatc agcaccattg tattggtgcc agttatcatc gcggcagcga
    36241 agagacggcg tacagtgaag acaatcagca gcagaatcgc cagcgattga ttgattgttt
    36301 cccacacgca caatgggcga agacggttga tgtcagtaaa aaagaggcgc gctgcggtgt
    36361 gcgttgtgcc acccgcgatc atctgccaat ggtaggtaat gttcccgatt atgaggcaac
    36421 actcgtggaa tatgcgtcgc tggcggagca gaaagataag gcggtaagcg cgccggtttt
    36481 tgacgatctc tttatgtttg ctgctttagg ttcgcgcggt ttgtgttctg ccccgctgtg
    36541 tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct
    36601 ggcggcgtta aatccgaatc ggttatgggt gcggaaattg ttgaaaggta aagcggttaa
    36661 ggccgggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccgacct
    36721 acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag
    36781 acgccgcaac ttactgctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca
    36841 gcgcctggtc acgcggcgac aattcaccag caccaatggc tttttccaga ctctggctaa
    36901 cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta
    36961 atgtaaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa
    37021 ccataccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc
    37081 attgtaaaac gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg
    37141 taagttccgg cgtgtgataa taattttgta gtgctttaat gaaacgtgcc gggcgttccg
    37201 gaatgccatt tgttagataa tccatcacct gcgcatgtac tcgccgttga aacaccacgc
    37261 ggtccggttc gaagtcgcct tccagattgt cgcaactgac gttaaacggg tatcccgccg
    37321 ccacgcagaa caaccagtcg agcgcctgcg gcttcacttc aacatcttca aactggcttt
    37381 gcgtttgggc atcgcgaccg tccgggcaat accagtagcc gaaatcaacc tgctcgcggc
    37441 gcgctttccc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccgt
    37501 gggcaaagac gattcggtta tacggtactt ccgcatcagc gggaagatag atcggttcgt
    37561 cgtcgccttt aatcagacgg gtattaaaat catcggcaaa gcagctatta aaaatttcaa
    37621 tcaactgctc atagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc
    37681 ctgtccgtgg ctatcataaa gtaatttggc actcatcacc gccgagacga taacgatcat
    37741 cgggcggatc agtttttgtc ctttgctcaa caccagtctt gaacccatgc gcgcgccaag
    37801 gaactgcccc accagcatca caaagcccgt cgcccaaatc actttgccgc cgagaataaa
    37861 cagcagtaaa ccgccgatgt ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt
    37921 ggcgaggttg aatccgcaca gcgtaacaaa ggccagcgcg taaaacgatc cggctgccgg
    37981 accaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga agggcaaacc
    38041 gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc
    38101 gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac
    38161 caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattaagtt tctgatcgct
    38221 taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc
    38281 ttgcagttta ttggttgcca gagcattagc gggagacatc cccgctgcca tcaatgccgg
    38341 aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac
    38401 aaaaaagaga actcccagca acagcgggga aaccataaac aggctattaa acgtttccat
    38461 tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc
    38521 ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg
    38581 caaccatcgc ctggtggcgg taaaggttga tcttcacact ccagactatc ggcaggacaa
    38641 cgtaatcgta catgcatatg cgcacgatgc tggaaccagg gtcgcacttt gcgcaaccag
    38701 tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg
    38761 cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac
    38821 agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag
    38881 agctgcgcgg aggtccggcg tgttttcggc agttgcagaa agatatcgac atccagtccg
    38941 gtctggtggc tggcgtgacc gccgttgaaa cgcccaccag caggcattcc catatcgcca
    39001 atcaacaccg tacccatgcc cagattgctc acctgactac tcaggcgctg gataaacatc
    39061 accaggtccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg
    39121 gactgtatcg gcaacgtgtc agcgccgacg atacaaccat tggaaaaact gcctatcgat
    39181 tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctgacg
    39241 ctactggcaa gcagggccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga
    39301 atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg
    39361 atcgccagca tcgcttctgc tatcggcacc gcgcggatcc cgacacaggg atcgtgacgg
    39421 cctttggtga tcatctcaac ttcttcgcca aagcggttaa tggtgcgccc cggcacggta
    39481 atactggagg tcggtttcag tgccatatga gcaatgattt gctgcccgct gctaattccg
    39541 ccgagaatgc cgcccgcatg gttactctgg aaaccgtctt tggtgatttc gtcgcggttc
    39601 tggctgccac gcagcgccac cacgtcaaaa ccatcaccaa tttccacgcc tttcaccgcg
    39661 ttaatgctca ttagcgcatg ggcgatgtcg gcatccaggc gatcaaagac cggctcacca
    39721 agcccggcag gaacgccact ggcaacaacg gtgactttcg cgccaataga gtcgccctct
    39781 tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtccgggtc cgggcagaag
    39841 aatggatttt gctcgacctg cgaccagtct ttgatttcca gcggaatgtc gcccatctgg
    39901 gtcaggcagc cgcgaatttc gatgccaaat ttctcagcga gatatttttt ggcaatcgcc
    39961 cctgctgcaa cgcgcatggc ggtttcacgg gcggaagaac gtccaccgcc gcgataatcg
    40021 cgcagaccgt atttttgttc gtaggtgtaa tcggcatggc caggacggaa aacgtcctta
    40081 atcgcactat aatcctgaga acgctgatcg gtattttcga tcaacaaacc aatgctggtg
    40141 ccggtagtaa caccttcaaa aacaccggag agaattttga cctgatctgg ctcacgacgc
    40201 tgggtggtat agcgcgatgt cccagggcga cgacggtcga ggtcatgttg caggtccgct
    40261 tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc
    40321 gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct
    40381 ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttcacgggc
    40441 cgccagcagc tgcgctttgg tgagcataaa cacaccatcg ccgccgttat caaactccag
    40501 ccaggtgaac ggaacatcgg gatattgttc cataagatgt accatgctgt tgccgacttc
    40561 acaaatcagt acgccatcat cagcaagata atctgccgcg tttccgagaa tgcgacgcgt
    40621 cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtggc ggtattcatt
    40681 tggcaggtcg gacatatctt ccgcatcgac atacggcggg ttagtgacaa tcaggtcgta
    40741 ctgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggtatgacgt tgtggatcag
    40801 accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc
    40861 atcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt
    40921 acacatatct aaaatatgct gcggttgctt gctgataagt ccggcaaatt tattgttgat
    40981 caattcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatggcc
    41041 gcagaaccac gctttgttgg tcaggtaagc taccggaatg cgttcgttga cgcggcggat
    41101 cacgcgctca acaatacgat gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc
    41161 cggaatatcc agcggcaggt agagcgacgg caataccagc tgtacggctt catcccacgg
    41221 gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctca ccgaccagcg
    41281 caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac
    41341 gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga
    41401 taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgcgct
    41461 acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca
    41521 acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt
    41581 aagcaggaca ccattgtcca tcggccacag cgtaaaaaag tcagcgaagt gcccgtcaaa
    41641 cggttgattc aggagcaggt tgacgccagt cactatttct ctgacgaatt tcaaccgtta
    41701 ttaaatgctg atggcccggt gaagtatgta cgccctggtg tagaccattt tgaggcgaaa
    41761 aagctgcgcc gcggcgatta ttcgccggaa ttgtttttgg atttacacgg tctgacgcag
    41821 ctgcaggcca agcaggaact gggcgcgttg atcgccgcct gtcgccgcga acatgtgttt
    41881 tgcgcctgcg tgatgcatgg tcacgggaaa catatattga agcaacaaac accgctgtgg
    41941 ctggcacaac atccgcatgt catggcgttt caccaggcac caaaagagta tggtggtgat
    42001 gccgcattgc tggtgttgat tgaagtggaa gagtggctac cgccggagtt gccatgaata
    42061 ttagggggcg gatgcgtttt gccatttcgc cctgaatgct ggtatcgaat gccggatgcg
    42121 gcgtaaacgc ctgatccggc ttatcaaaat gtgcaaattc aatacgttgt gattcagttg
    42181 taggcatgat aagacgcgtc agcgtcgcat caggcatcaa cgcacaacta cagttcattc
    42241 agtacatact ggaaacggca tgtagaatca attggctcac ggctactggt attcacctgt
    42301 accacaatgt cgtgtagctt tctatccgtc aatggttgac gtgagggggt gactgtcacc
    42361 tttgcattat cacaaccata cgaggtaaag gttaataagt cagaaacagc acgacaatat
    42421 gcccctctgg aatcaaaaat actgccatcg cagagtttgc cgctttccat caggtaggtc
    42481 gaaagcgtag tcgtgattgt cgaaccatcg gattgcaatc gcagcgcttt attatggcta
    42541 acataattat cactccagga tatatcaaac gaagcgacgg gaattgtcgg gcaaatagaa
    42601 tcatgaactg ttgggcttgt atacgtacca aatccaaatg cctcagatac tgaataaaat
    42661 gaggcaatcc acggacaacc ataattatca gattctacag tgacgttaaa agtcatatat
    42721 gcacagacaa agacacgagt attgatacca gggcaatttg aggtgtccgt acgtctataa
    42781 tcagtcaaaa ccagtgtcgg tgccgggcca gatccgctcc aggatattaa cttgagggtg
    42841 tcgccttttc ttgcattgga gttgtattgt ccgtagctta cttctcccgg cgtcactacc
    42901 gtgacataga aatataaact gccggaagca gtaacgtttt tcatcgcata ataaatttta
    42961 gtactggaaa agcttgcact catgagttta tcaccggcat agcactttgg tgtttctaaa
    43021 ctgagagcaa gtaatatcag acttgtcaaa aatatcgtcc attttttcat tttaaaaata
    43081 ctcaaaagtt accgtcattg tggcggtgaa atcacccgat gtaacatttc gtccatttac
    43141 ggtctgtaac caggcattaa aattgacact gttatttccc tgatttaatg gaaatgaggc
    43201 acctgtggta ctgttaatag gtacagccgc ccctccggca gtttcaatgc caatcccaac
    43261 acccgttgca gaagatgacg aatcaatcga cagaaatcct ggtaagtcgg tatcttctgt
    43321 tcccatcaag gtcaccttga cattaaacgc cgtggtgctt ttgcaatctt tcaactgaaa
    43381 aactaacggt acgcgatctg actgaccacg ttgcattaaa tcgctggcag caattgtggg
    43441 gaaatgaatt tctgcaagta agttgc
//
LOCUS       DAZFTN010000024        43414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFTN010000024 DAZFTN010000000
VERSION     DAZFTN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            136..1704
                     /gene="cydA"
                     /locus_tag="ACHKV2_001524"
     CDS             136..1704
                     /gene="cydA"
                     /locus_tag="ACHKV2_001524"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO6939437.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1720..2859
                     /gene="cydB"
                     /locus_tag="ACHKV2_001525"
     CDS             1720..2859
                     /gene="cydB"
                     /locus_tag="ACHKV2_001525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6939438.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2874..2987
                     /gene="cydX"
                     /locus_tag="ACHKV2_001526"
     CDS             2874..2987
                     /gene="cydX"
                     /locus_tag="ACHKV2_001526"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO6939439.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            2987..3280
                     /gene="ybgE"
                     /locus_tag="ACHKV2_001527"
     CDS             2987..3280
                     /gene="ybgE"
                     /locus_tag="ACHKV2_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO6939440.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3430..3834
                     /gene="ybgC"
                     /locus_tag="ACHKV2_001528"
     CDS             3430..3834
                     /gene="ybgC"
                     /locus_tag="ACHKV2_001528"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6939441.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3831..4523
                     /gene="tolQ"
                     /locus_tag="ACHKV2_001529"
     CDS             3831..4523
                     /gene="tolQ"
                     /locus_tag="ACHKV2_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO6939442.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4527..4955
                     /gene="tolR"
                     /locus_tag="ACHKV2_001530"
     CDS             4527..4955
                     /gene="tolR"
                     /locus_tag="ACHKV2_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO6939443.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5020..6234
                     /gene="tolA"
                     /locus_tag="ACHKV2_001531"
     CDS             5020..6234
                     /gene="tolA"
                     /locus_tag="ACHKV2_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6939444.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAE
                     ADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESK
                     FYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVY
                     EVFKNAPLDFKP"
     gene            6367..7659
                     /gene="tolB"
                     /locus_tag="ACHKV2_001532"
     CDS             6367..7659
                     /gene="tolB"
                     /locus_tag="ACHKV2_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO6939445.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7694..8215
                     /gene="pal"
                     /locus_tag="ACHKV2_001533"
     CDS             7694..8215
                     /gene="pal"
                     /locus_tag="ACHKV2_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO6939446.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8225..9016
                     /gene="cpoB"
                     /locus_tag="ACHKV2_001534"
     CDS             8225..9016
                     /gene="cpoB"
                     /locus_tag="ACHKV2_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO6939447.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9181..9256
                     /locus_tag="ACHKV2_001535"
     tRNA            9181..9256
                     /locus_tag="ACHKV2_001535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9214..9216,aa:Lys,seq:ttt)
     gene            9292..9367
                     /locus_tag="ACHKV2_001536"
     tRNA            9292..9367
                     /locus_tag="ACHKV2_001536"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9325..9327,aa:Val,seq:tac)
     gene            9370..9445
                     /locus_tag="ACHKV2_001537"
     tRNA            9370..9445
                     /locus_tag="ACHKV2_001537"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9403..9405,aa:Lys,seq:ttt)
     gene            9497..9572
                     /locus_tag="ACHKV2_001538"
     tRNA            9497..9572
                     /locus_tag="ACHKV2_001538"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9530..9532,aa:Val,seq:tac)
     gene            9576..9651
                     /locus_tag="ACHKV2_001539"
     tRNA            9576..9651
                     /locus_tag="ACHKV2_001539"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9609..9611,aa:Lys,seq:ttt)
     gene            9798..9873
                     /locus_tag="ACHKV2_001540"
     tRNA            9798..9873
                     /locus_tag="ACHKV2_001540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9831..9833,aa:Lys,seq:ttt)
     gene            10151..11194
                     /gene="nadA"
                     /locus_tag="ACHKV2_001541"
     CDS             10151..11194
                     /gene="nadA"
                     /locus_tag="ACHKV2_001541"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO6939448.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11232..11951
                     /gene="pnuC"
                     /locus_tag="ACHKV2_001542"
     CDS             11232..11951
                     /gene="pnuC"
                     /locus_tag="ACHKV2_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO6939449.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11948..12889)
                     /gene="zitB"
                     /locus_tag="ACHKV2_001543"
     CDS             complement(11948..12889)
                     /gene="zitB"
                     /locus_tag="ACHKV2_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO6939450.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13003..13383)
                     /gene="ybgS"
                     /locus_tag="ACHKV2_001544"
     CDS             complement(13003..13383)
                     /gene="ybgS"
                     /locus_tag="ACHKV2_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO6939451.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13699..14751
                     /gene="aroG"
                     /locus_tag="ACHKV2_001545"
     CDS             13699..14751
                     /gene="aroG"
                     /locus_tag="ACHKV2_001545"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO6939452.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(14917..15669)
                     /gene="gpmA"
                     /locus_tag="ACHKV2_001546"
     CDS             complement(14917..15669)
                     /gene="gpmA"
                     /locus_tag="ACHKV2_001546"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO6939453.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15871..16911)
                     /gene="galM"
                     /locus_tag="ACHKV2_001547"
     CDS             complement(15871..16911)
                     /gene="galM"
                     /locus_tag="ACHKV2_001547"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO6939454.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(16905..18053)
                     /gene="galK"
                     /locus_tag="ACHKV2_001548"
     CDS             complement(16905..18053)
                     /gene="galK"
                     /locus_tag="ACHKV2_001548"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO6939455.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18057..19103)
                     /gene="galT"
                     /locus_tag="ACHKV2_001549"
     CDS             complement(18057..19103)
                     /gene="galT"
                     /locus_tag="ACHKV2_001549"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO6939456.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19113..20129)
                     /gene="galE"
                     /locus_tag="ACHKV2_001550"
     CDS             complement(19113..20129)
                     /gene="galE"
                     /locus_tag="ACHKV2_001550"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6939457.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20391..21863)
                     /gene="modF"
                     /locus_tag="ACHKV2_001551"
     CDS             complement(20391..21863)
                     /gene="modF"
                     /locus_tag="ACHKV2_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO6939458.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(21931..22719)
                     /gene="modE"
                     /locus_tag="ACHKV2_001552"
     CDS             complement(21931..22719)
                     /gene="modE"
                     /locus_tag="ACHKV2_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO6939459.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22848..22997
                     /gene="acrZ"
                     /locus_tag="ACHKV2_001553"
     CDS             22848..22997
                     /gene="acrZ"
                     /locus_tag="ACHKV2_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO6939460.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23164..23937
                     /gene="modA"
                     /locus_tag="ACHKV2_001554"
     CDS             23164..23937
                     /gene="modA"
                     /locus_tag="ACHKV2_001554"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6939461.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            23937..24626
                     /gene="modB"
                     /locus_tag="ACHKV2_001555"
     CDS             23937..24626
                     /gene="modB"
                     /locus_tag="ACHKV2_001555"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO6939462.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24629..25687
                     /gene="modC"
                     /locus_tag="ACHKV2_001556"
     CDS             24629..25687
                     /gene="modC"
                     /locus_tag="ACHKV2_001556"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO6939463.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25688..26506)
                     /gene="ybhA"
                     /locus_tag="ACHKV2_001557"
     CDS             complement(25688..26506)
                     /gene="ybhA"
                     /locus_tag="ACHKV2_001557"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO6939464.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26661..27656
                     /gene="pgl"
                     /locus_tag="ACHKV2_001558"
     CDS             26661..27656
                     /gene="pgl"
                     /locus_tag="ACHKV2_001558"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO6939465.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27697..28650)
                     /gene="ybhD"
                     /locus_tag="ACHKV2_001559"
     CDS             complement(27697..28650)
                     /gene="ybhD"
                     /locus_tag="ACHKV2_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6939466.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28834..29886
                     /gene="ybhH"
                     /locus_tag="ACHKV2_001560"
     CDS             28834..29886
                     /gene="ybhH"
                     /locus_tag="ACHKV2_001560"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO6939467.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            29962..31395
                     /gene="ybhI"
                     /locus_tag="ACHKV2_001561"
     CDS             29962..31395
                     /gene="ybhI"
                     /locus_tag="ACHKV2_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO6939468.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31578..33839
                     /gene="ybhJ"
                     /locus_tag="ACHKV2_001562"
     CDS             31578..33839
                     /gene="ybhJ"
                     /locus_tag="ACHKV2_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO6939469.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(33980..35263)
                     /gene="ybhC"
                     /locus_tag="ACHKV2_001563"
     CDS             complement(33980..35263)
                     /gene="ybhC"
                     /locus_tag="ACHKV2_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO6939470.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35398..36464)
                     /locus_tag="ACHKV2_001564"
                     /pseudo
     CDS             complement(35398..36464)
                     /locus_tag="ACHKV2_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(36442..36660)
                     /locus_tag="ACHKV2_001565"
     CDS             complement(36442..36660)
                     /locus_tag="ACHKV2_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6939471.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36700..36867)
                     /locus_tag="ACHKV2_001566"
     CDS             complement(36700..36867)
                     /locus_tag="ACHKV2_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939472.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            37110..37712
                     /locus_tag="ACHKV2_001567"
     CDS             37110..37712
                     /locus_tag="ACHKV2_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939473.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(37923..38144)
                     /locus_tag="ACHKV2_001568"
     CDS             complement(37923..38144)
                     /locus_tag="ACHKV2_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6939474.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(38243..38458)
                     /locus_tag="ACHKV2_001569"
     CDS             complement(38243..38458)
                     /locus_tag="ACHKV2_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6939475.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(38535..38726)
                     /locus_tag="ACHKV2_001570"
     CDS             complement(38535..38726)
                     /locus_tag="ACHKV2_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO6939476.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(38699..38881)
                     /locus_tag="ACHKV2_001571"
     CDS             complement(38699..38881)
                     /locus_tag="ACHKV2_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO6939477.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(38878..39558)
                     /locus_tag="ACHKV2_001572"
     CDS             complement(38878..39558)
                     /locus_tag="ACHKV2_001572"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6939478.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(39555..40226)
                     /gene="bet"
                     /locus_tag="ACHKV2_001573"
                     /pseudo
     CDS             complement(39555..40226)
                     /gene="bet"
                     /locus_tag="ACHKV2_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            40256..40438
                     /locus_tag="ACHKV2_001574"
     CDS             40256..40438
                     /locus_tag="ACHKV2_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6939479.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            40435..40605
                     /locus_tag="ACHKV2_001575"
     CDS             40435..40605
                     /locus_tag="ACHKV2_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO6939480.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            40598..41218
                     /locus_tag="ACHKV2_001576"
     CDS             40598..41218
                     /locus_tag="ACHKV2_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO6939481.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            41215..41880
                     /locus_tag="ACHKV2_001577"
     CDS             41215..41880
                     /locus_tag="ACHKV2_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO6939482.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            41877..42500
                     /locus_tag="ACHKV2_001578"
     CDS             41877..42500
                     /locus_tag="ACHKV2_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6939483.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            42753..>43414
                     /locus_tag="ACHKV2_001579"
     CDS             42753..>43414
                     /locus_tag="ACHKV2_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939484.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SF"
BASE COUNT        10528 a        10469 c        11238 g        11179 t
ORIGIN      
        1 ttatcattgt cttttcctct tttgatgaaa tggcaacgat agaggcgagc aaataatttt
       61 gtattggggc atgcgtgtga ccctttctaa cggggttcac tctcggagtc ttcatgcgat
      121 gagcaaggag tcatgatgtt agatatagtc gaactgtcgc gcttacagtt tgccttgacc
      181 gcgatgtacc acttcctttt tgtgccactg acgctcggta tggcgttcct gctggccatt
      241 atggaaacgg tctacgtcct ttccggcaaa cagatttata aagatatgac caagttctgg
      301 ggcaagttgt ttggtatcaa cttcgctctg ggtgtggcta ccggtctgac catggagttc
      361 cagttcggga ctaactggtc ttactattcc cactatgtag gggatatctt cggtgcgccg
      421 ctggcaatcg aaggtctgat ggccttcttc ctcgaatcca cctttgtagg tctgttcttc
      481 ttcggttggg atcgtctggg taaagttcag catatgtgtg tcacctggct ggtggcgctc
      541 ggttcaaacc tgtccgcact gtggattctg gttgcgaacg gctggatgca aaacccaatc
      601 gcgtccgatt tcaactttga aactatgcgt atggagatgg tgagcttctc cgagctggtg
      661 cttaacccgg ttgctcaggt gaaattcgtt cacactgtag cgtctggtta tgtgactggc
      721 gcgatgttca tcctcggtat cagcgcatgg tatatgctga aaggtcgtga cttcgccttc
      781 gctaaacgct cctttgctat cgctgccagc ttcggtatgg ctgccgttct gtctgttatt
      841 gttctgggtg atgaatccgg ctacgaaatg ggcgacgtgc agaaaaccaa actggctgct
      901 attgaagctg agtgggaaac gcaacctgcg cctgctgcct ttactctgtt cggcattcct
      961 gatcaggaag aggagacgaa caaatttgcg atccagatcc cttacgcgct gggcataatt
     1021 gcaacgcgtt ccgtggatac tccagttatc ggcctgaaag aactgatggt gcagcatgaa
     1081 gaacgcattc gtaacgggat gaaggcgtac tctctgctcg aacaactgcg ttctggttct
     1141 accgaccagg cggttcgtga ccagttcaat agcatgaaga aggacctcgg ttacggtctg
     1201 ctgctgaaac gctatacgcc aaacgtggct gatgcgactg aagcgcagat tcaacaggca
     1261 accaaagact ccattccgcg tgtagcgccg ctgtacttcg cgttccgtat catggtggcg
     1321 tgtggcttcc tgctgctggc aatcatcgcg ctctctttct ggagtgtcat ccgcaaccgc
     1381 attggcgaga aaaaatggct cctgcgcgcc gcgctgtacg gtattccgct gccgtggatt
     1441 gctgtagaag cgggctggtt cgtggctgaa tatggccgcc aaccgtgggc tatcggtgaa
     1501 gtgctgccga cagctgtggc gaactcgtca ctgaccgcag gcgatctcat cttctcaatg
     1561 gtgctgattt gcggcctgta taccctgttc ctggtggcag aattgttctt aatgttcaag
     1621 tttgcacgcc tcggcccaag cagcctgaaa accggtcgct atcactttga gcagtcttcc
     1681 acgactactc agccggcacg ctaagacagg agtcgtcaaa tgatcgatta tgaagtattg
     1741 cgttttatct ggtggctgct ggttggcgtt ctgctgattg gttttgcagt cactgatggt
     1801 ttcgacatgg gggtgggcat gctcacccgt ttcctcggtc gtaacgacac cgagcgtcga
     1861 attatgatta actccatcgc accacactgg gacggtaacc aggtttggct gatcaccgca
     1921 ggcggcgcac tgtttgctgc ctggccgatg gtctatgccg ctgcgttctc cggcttctat
     1981 gtggcgatga tcctcgtgct ggcgtctttg ttcttccgtc cggtcggttt tgactaccgc
     2041 tccaagattg aagaaacccg ctggcgtaac atgtgggact ggggcatctt cattggtagc
     2101 ttcgttccgc cgttggtgat tggtgtagcg ttcggtaacc tgttgcaggg cgtaccgttc
     2161 aacgttgatg aatatctgcg tctgtactac accggtaact tcttccagct gcttaacccg
     2221 ttcggcctgc tggcaggcgt ggtgagcgta gggatgatca ttactcaggg cgcaacctat
     2281 ctgcaaatgc gtaccgtggg cgaactgcac ctgcgtaccc gtgcaacggc tcaggtggcg
     2341 gcactggtga cactggtctg tttcgcactg gctggcgtat gggtgatgta cggtatcgat
     2401 ggttatgtcg tgaaatcgac aatggaccat tacgcagcct ctaacccgct gaataaagaa
     2461 gtggttcgtg aagctggcgc atggctggtt aacttcaaca acacgccaat tctgtgggct
     2521 attccggcac tgggtgtggt tctgccgctg ctgaccatcc tgactgcacg tatggataaa
     2581 gccgcgtggg cgtttgtgtt ctcctccctg acgctggcct gcatcatcct gactgccggt
     2641 atcgcaatgt tcccgtttgt gatgccgtcc agcaccatga tgaacgcaag tctgacaatg
     2701 tgggatgcaa cttccagcca gctgacactt aacgtcatga cctgggttgc ggtggttctg
     2761 gtaccgatca ttctgctcta caccgcctgg tgttactgga aaatgttcgg tcgtatcacc
     2821 aaagaagata ttgaacgtaa cacccactct ctgtactaag taaggagcta aaaatgtggt
     2881 atttcgcatg gattctggga acgcttcttg cctgttcgtt tggggtaatc accgcgctgg
     2941 cgcttgagca cgtcgaatca ggcaaagccg gtcaagaaga catctgatga gtaagattat
     3001 cgcaaccttg tatgcggtaa tggacaagcg ccccctgcgg gcgctttcct tcgtgatggc
     3061 gcttctgtta gcaggatgta tgttttggga cccatctcgt ttcgccgcga agaccagtga
     3121 actggaaatc tggcatggtt tattgctgat gtgggccgtc tgtgctggtg tgattcacgg
     3181 cgtgggcttt cgtccgcaga aggttctttg gcaagggatt ttttgcccat tgcttgccga
     3241 tattgttctc attgtcgggc tgattttctt cttcttttaa atcagaattc tctttaaaaa
     3301 attatgggcc gtgccaggcc cataaatttt tacgctccct taacttgccc tcattcccaa
     3361 acctcaatcg cgcgcgtata gtagcagcgt ttaaaagctc taacttttgt tgcattaccg
     3421 ggatgtaaag tgaatacaac gctgtttcga tggccggttc gcgtctacta tgaagatacc
     3481 gatgccggtg gtgtggtgta ccacgccagt tacgtcgctt tttatgaaag agcacgcaca
     3541 gagatgctgc gtcatcatca cttcagtcaa caggcgctga tggctgaacg cgttgccttt
     3601 gtggtacgta aaatgacggt ggaatattac gcacctgcgc ggctcgacga tatgctcgaa
     3661 atacagactg aaataacatc aatgcgtggc acctctttgg ttttcacgca acgtattgtc
     3721 aacgccgaga atactttgct gaatgaagca gaggttctgg ttgtttgcgt tgacccactc
     3781 aaaatgaagc ctcgtgcgct tcccaagtct attgtcgcgg agtttaagca gtgactgaca
     3841 tgaatatcct tgatttgttc ctgaaggcta gccttctggt taaacttatc atgttgattt
     3901 tgattggttt ttcaatcgca tcctgggcca ttattatcca gcggacccgt attcttaacg
     3961 cagcggcgcg cgaagccgaa gcgtttgaag ataaattctg gtctggaatc gaactctctc
     4021 gcctctatca agagagccag gggaaacggg ataatctgac tggttcggaa caaatctttt
     4081 acagcgggtt caaagaattt gtgcgcctgc atcgtgccaa tagccatgcg ccggaagccg
     4141 tagtggaagg ggcgtcgcgt gcgatgcgta tttccatgaa ccgcgaactt gaaaatctgg
     4201 aaacgcacat tccgttcctc ggtacggttg gctccatcag cccgtatatt ggtctgtttg
     4261 gtacggtctg ggggatcatg cacgccttta tcgccctcgg ggcggtaaaa caagcaacac
     4321 tgcaaatggt tgcgcccggt atcgcagaag cgttgattgc gacggcaatt ggtctgttcg
     4381 ccgctattcc ggcggtaatg gcttataacc gtctcaacca acgtgtaaac aaactggaac
     4441 tgaattacga caactttatg gaagagttta ccgcgattct gcaccgccag gcgtttaccg
     4501 ttagcgagag caacaagggg taagccatgg ccagagcgcg tggacgaggt cgtcgcgatc
     4561 tcaagtccga aatcaacatt gtaccgttgc tggacgtact gctggtgctg ttgctgatct
     4621 ttatggcgac agcgcccatc atcacccaga gcgtggaggt cgatttgcca gacgctactg
     4681 aatcacaggc ggtgagcagt aacgataatc cgccagtgat tgttgaagtg tctggtattg
     4741 gtcagtacac cgtggtggtt gagaaagatc gcctggagcg tttaccacca gagcaggtgg
     4801 tggcggaagt gtccagccgt ttcaaggcca acccgaaaac ggtctttctg atcggtggcg
     4861 caaaagatgt gccttacgat gaaataatta aagcactgaa cttgttacat agtgcgggtg
     4921 tgaaatcggt tggtttaatg acgcagccta tctaaacatc tgcgtttccc ttgcttgaaa
     4981 gagagcgggt aacaggcgaa cagtttttgg aaaccgagag tgtcaaaggc aaccgaacaa
     5041 aacgacaagc tcaagcgggc gataattatt tcagcagtgc tgcatgtcat cttatttgcg
     5101 gcgctgatct ggagttcgtt cgatgagaat atagaagctt cagctggagg cggcggtggt
     5161 tcgtccatcg acgctgtcat ggttgattca ggtgcggtag ttgagcagta caaacgcatg
     5221 caaagccagg aatcaagcgc gaagcgttct gatgagcagc gcaagatgaa ggaacagcag
     5281 gctgctgaag aactgcgtga gaaacaagcg gctgaacagg aacgcctgaa gcaacttgag
     5341 aaagagcggt tagctgctca ggaacagaaa aagcaggctg aagaagccgc aaaacaggcc
     5401 gagttaaagc agaagcaagc ggaagaggcg gcagcgaaag cggcggcaga tgctaaagcg
     5461 aaggccgaag cggatgataa agctgcggaa gaagcagcga agaaagcggc tgcagacgcg
     5521 aagaaaaaag cagaagcaga agccgccaaa gccgcagccg aagcgcagaa aaaagccgag
     5581 gcagcagctg cggcgctgaa gaagaaagcg gaagcggcag aagcagctgc agctgaagca
     5641 agaaagaaag cggcagcaga gaaagctgca gccgacaaaa aagcagcaga gaaagctgca
     5701 gccgacaaaa aagcagcaga aaaagcggct gctgaaaagg cagcagctga taagaaagca
     5761 gcggcagaaa aagccgccgc agacaaaaaa gcggcagctg caaaagcagc agctgaaaaa
     5821 gccgctgcag caaaagctgc cgcggaggca gatgatattt tcggtgagct aagctctggt
     5881 aagaatgcac cgaaaacggg gggaggggcg aaagggaaca atgcttcgcc tgccgggagt
     5941 ggtaatacta aaaacaatgg cgcatcaggg gccgatatca ataactatgc cgggcagatt
     6001 aaatctgcta tcgaaagtaa gttctatgac gcatcgtcct atgcaggcaa aacctgtacg
     6061 ctgcgcataa aactggcacc cgatggtatg ttactggata tcaaacctga aggtggcgat
     6121 cccgcacttt gtcaggctgc gttggcagca gctaaacttg cgaagatccc gaaaccacca
     6181 agccaggcag tatatgaagt gttcaaaaac gcgccattgg acttcaaacc gtaatcgcga
     6241 tgttgactgt tccgacggtc aacatcaggc accggttgcc acggggttct ggtagttttg
     6301 tgtattttag tttgttaaca ttctgctaaa ttatcgtggg ccatcggtcc agataaggga
     6361 gatatgatga agcaggcatt acgagtagca tttggttttc tcatactgtg ggcatcagtt
     6421 ctgcatgctg aagtccgcat tgtgatcgac agcggtgtag attccggtcg tcctattgga
     6481 gttgttcctt tccagtgggc gggccctggt gcggcacctg aagatattgg cggcatcgtt
     6541 gctgccgact tgcgtaacag cggtaaattt aatccgttag atcgcgctcg tctgccacag
     6601 cagccgggta gtgcgcagga agtacaacca gctgcatggt ccgcgctggg tattgacgct
     6661 gtagttgtcg gtcaggtcac tccgaatccg gatggttctt acaatgttgc ttatcaactt
     6721 gttgacactg gcggcgcacc gggtactgta cttgctcaga actcgtacaa agtgaacaag
     6781 cagtggctgc gttatgctgg tcataccgcc agtgatgaag tgtttgaaaa actgaccggt
     6841 attaaaggtg cgttccgtac ccgtattgcc tacgttgttc agaccaacgg cggtcagttc
     6901 ccgtatgaac tgcgcgtatc tgactatgac ggttacaacc agtttgtcgt tcaccgttca
     6961 ccacagccgc tgatgtcccc ggcgtggtca ccagacggtt ctaaactggc ttatgtgacc
     7021 ttcgaaagcg gtcgttccgc gctggttata caaacgctgg caaacggcgc tgtacgtcag
     7081 gtggcttcat tcccgcgtca caacggtgcg cctgcattct cgccagacgg cagcaaactg
     7141 gcattcgcct tgtcgaaaac cggtagcctg aacctgtacg taatggattt ggcttctggt
     7201 cagatccgcc aggtgactga tggtcgcagt aacaataccg aaccgacctg gttcccggac
     7261 agccagaacc tggcatttac ttctgaccag gccggtcgtc cacaggttta taaagtgaat
     7321 atcaacggcg gtgcgccaca acgtattacc tgggaaggtt cgcagaacca ggatgcggat
     7381 gtcagcagcg acggtaaatt tatggtaatg gtcagctcca atggtgggca gcagcacatt
     7441 gccaaacaag atctggcaac gggaggcgta caagttctgt cgtccacgtt cctggatgaa
     7501 acgccaagtc tggcacctaa cggcactatg gtaatctaca gctcttctca ggggatggga
     7561 tccgtgctga atttggtttc tacagatggg cgtttcaaag cgcgtcttcc ggcaactgat
     7621 ggacaggtca aattccctgc ctggtcgccg tatctgtgat aataattaat tgaatagtaa
     7681 aggaatcatt gaaatgcaac tgaacaaagt gctgaaaggg ctgatgattg ctctgcctgt
     7741 tatggcaatt gcggcatgtt cttccaacaa gaacgccagc aatgacggca gcgaaggcat
     7801 gctgggtgcc ggcactggta tggatgcgaa cggcggcaac ggcaacatgt cttccgaaga
     7861 gcaggctcgt ctgcaaatgc aacagctgca gcagaacaac atcgtttact tcgatctgga
     7921 caagtacgat atccgttctg acttcgctca aatgctggat gcacatgcaa acttcctgcg
     7981 tagcaacccg tcttacaaag tcaccgtaga aggtcacgcg gacgaacgtg gtactccgga
     8041 atacaacatc tccctgggtg aacgtcgtgc gaacgccgtt aagatgtacc tgcagggtaa
     8101 aggcgtttct gcagaccaga tctccatcgt ttcttacggt aaagaaaaac ctgcagtact
     8161 gggtcatgac gaagcggcat actccaaaaa ccgtcgtgcg gtactggttt actaagagaa
     8221 ttgcatgagc agtaacttca gacatcaact attgagtctg tcgttactgg ttggtatagc
     8281 ggccccctgg gccgcttttg ctcaggcacc aatcagtagt gtcggctcag gctcggtcga
     8341 agaccgcgtc attcaacttg agcgtatttc taatgctcac agccagcttt taacccaact
     8401 ccagcaacaa ctctctgata atcaatccga tattgattcc ctgcgtggtc agattcagga
     8461 aaatcagtat caactgaatc aggtcgtgga gcggcaaaag cagatcctgt tgcagatcga
     8521 cagcctcagc agcggtggtg cagcggcgca atcaaccagc ggtgatcaaa gcggtgcggc
     8581 ggcatcaacg acgccgacag ctgatgctgg tactgcgaat gctggcgcgc cggtgaaaag
     8641 cggtgatgca aacacggatt acaatgcagc tattgcgctg gtgcaggata aatcccgcca
     8701 ggatgacgca atggtggcat ttcagaattt catcaaaaat taccctgatt caacttacct
     8761 gccaaacgcc aattattggc tgggtcagtt aaactacaac aagggtaaaa aagatgatgc
     8821 ggcgtactat tttgcttcgg tagtgaaaaa ctatccgaag tcaccaaagg ctgcagatgc
     8881 gatgtttaaa gtcggcgtca tcatgcagga caaaggtgac accgcaaaag cgaaagccgt
     8941 ataccagcag gttatcagta aataccctgg tactgatggc gctaaacagg cacaaaaacg
     9001 tctgaacgcg atgtaatgca taacacacga ccagaagtcg cattatttct ggtcgtgtcg
     9061 tgcgaatcat aagcagttga gtgatctaca tcgaaatttt tgttgcgctc aagtctgaaa
     9121 tcagtaatat atgccgccgt tgccacggga tatcaaacaa accgaaagca acgaaaaagt
     9181 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
     9241 cctgcacgac ccaccactaa cctagttagt tgtagtatcc agcgtaatat cgggtgatta
     9301 gctcagctgg gagagcacct cccttacaag gaggggggcg gcggttcgat cccgtcatca
     9361 cccaccaccg ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca
     9421 ggttcgaatc ctgcacgacc caccactaat tacggtgggt tcggtggaag tagtttgtag
     9481 tatccagcgc agtatcgggt gattagctca gctgggagag cacctccctt acaaggaggg
     9541 ggtcggcggt tcgatcccgt catcacccac cactcgggtc gttagctcag ttggtagagc
     9601 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aattttaaca
     9661 tcaaactcag atgttaagcg tgaaggataa cgttgcgtca gcaacggccc gtagggcaag
     9721 cgaagcgagt catcctgcac gacccaccaa ttttaaagat tggttccgag tagaaatctt
     9781 tcaggtaaca cccgtatggg tcgttagctc agttggtaga gcagttgact tttaatcaat
     9841 tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt
     9901 ttttgctatt caggcatctc tcaatttcaa ttcttgaccc tgatgacata ctccgcgctt
     9961 gctgcgtaaa caactttacg tcggaggaat aagacgcaaa gtgtggcatt cggcatctca
    10021 tacgtaattc gagtgacttt tccccaccat tcgactatct tgtttagcat ataaaacaaa
    10081 ttacaccgat aacagcgaat atcacgctaa tgtcggtttt aacgttaagc ctgtaaaacg
    10141 agatggtaag atgagcgtga tgtttgatcc agacacggcg atttatcctt tccccccgaa
    10201 gccgacgccg ttaagcattg atgaaaaagc gtattaccgc gagaagataa aacgtctgct
    10261 aaaagaacgt aatgcggtga tggttgccca ctactatacc gatcccgaaa ttcaacaact
    10321 ggcagaagaa accggtggct gtatttctga ttctctggaa atggcgcgct tcggtgcaaa
    10381 gcatcccgct tctacgttgt tagtcgctgg ggtgagattt atgggagaaa ccgccaaaat
    10441 tctcagtccg gaaaaaacaa ttctgatgcc gacacttcag gctgagtgtt cactggatct
    10501 cggctgccct gttgaagaat ttaacgcatt ttgcgatgcc catcccgatc gtactgtcgt
    10561 cgtctacgcc aacacttctg ctgcggtaaa agcgcgggca gattgggtgg taacttcaag
    10621 cattgccgtc gaacttattg atcatctgga tagtttgggt gaaaaaatca tctgggcacc
    10681 cgacaaacat ctggggcgtt acgtgcaaaa aaagacgggc gcagacattt tatgctggca
    10741 gggtgcctgc attgtgcatg atgagtttaa gactcaggcg ttaacccgct tgcaagaaga
    10801 atacccggat gctgccatac tggtgcatcc agaatcacca caagctattg tcgatatggc
    10861 ggatgcggtc ggttccacca gtcaactgat cgctgctgcg aaaacattgc cacatcagag
    10921 gcttattgtg gcaaccgatc ggggtatttt ctacaaaatg cagcaggcgg tgccagataa
    10981 agagttactg gaagcaccaa ccgcgggtga gggcgcaacc tgccgcagct gcgcgcattg
    11041 tccgtggatg gccatgaatg gcctccaggc catcgctgag gcattagaac aggaaggaag
    11101 caattacgag gttcatgttg atgaaaggct gcgagagagg gcgctggtgc cgctcaatcg
    11161 tatgctggat tttgcggcta cactacgtgg ataacgaata ataaggcgta acgttacgct
    11221 ttgggggaaa gatggatttt tttagtgtgc agaatatcct ggtacatata ccaataggag
    11281 caggcggtta tgatctctca tggatcgaag cggtaggcac aatcgccggg ttgctgtgta
    11341 ttggccttgc cagtctggag aagatcagca actacttctt tggcctgatc aacgtcacct
    11401 tgtttggcat tattttcttt cagattcagc tgtatgccag cctgctatta caggtgtttt
    11461 tctttgccgc gaatatttac ggctggtatg cgtggtcgcg acaaaccagt cagaacgagg
    11521 cggagttaaa aattcgctgg ttgccattgc cgaaggcact cagctggttg gcggtttgcg
    11581 ttgtttcgat tggtctgatg acggtattta tcaatccggt gtttgcattt ttgacccgcg
    11641 tggcagtcat catcatgcaa gcattaggat tacaggttgc gatgcctgaa ctacaaccgg
    11701 acgctttccc gttctgggat tcttgcatga tggtgttatc tatcgtggca atgattctga
    11761 tgactcgtaa gtatgtggaa aactggctgt tgtgggtgat tattaacgtg attagcgtcg
    11821 ttatttttgc acttcagggc gtttacgcca tgtctctgga gtacatcatc ctgaccttta
    11881 ttgcgctcaa cggcagccgg atgtggatca atagcgcacg cgaaagaggc tcacgcgcgc
    11941 tgtcccatta atggtgatga tgtgaatggc cagacacccc ttcattcagg tggcagtctg
    12001 gcccatgaca aggttgatat tccatctgaa tggtggcgtg ctcaatctga tagtgatcca
    12061 tcaggtagtg ttggatctga tccaacaagg catcgtgatc gtgtggcggg atcacctgca
    12121 catgcaacgt catcaccggc ttctcgccta ccatccatac atgcacatgg tgcacattgc
    12181 ggacttccgg gatttcccgg cacatacgac gcttcagttc agcgatatcc agcgataccg
    12241 gtgcaccctc aagtaattca ttcacgctat ctttcaacag tcgccacgcg ctacgcagaa
    12301 ccagaagcga caccagtatc gagagaatgg ggtcagcagg tgtccagccg gtccagataa
    12361 taatcaacgc ggcaataata gccccaaccg aacccagcag gtccccgagc acatgcagtg
    12421 ccgctgctcg tacgttgagg tttttctctt cgctgccgtg atgaagtaac cagaaagaaa
    12481 gtatatttgc cagcaaccca gccacggcaa ttgccatcat catgccgccc tcgaccggac
    12541 gcggcgtgcg gaagcgttct atcgcctccc agacaatcaa aatggtaatt accacgaggg
    12601 cgatggcatt cacaaacgcc gcgagcgtgg tcaatcttag ccaaccgaaa gtgtggcgaa
    12661 tggtgggagg acgacgtgaa aattgcacgg cgagtagggc gaaaagcagg gcggcagtat
    12721 cggtcaacat atgacccgca tcggccagca aagccagaga accggaaaga aaaccaccaa
    12781 tgacttctac cagcataaac ccggcagtca cgccgaaagc atacaacaag cgacgagcat
    12841 tattatcttc tggcaggtgt gaggacgtgt gtgagtgtga gtgcgccatg acgagtcatc
    12901 ccttaattat tctcacttaa ctttatgaca tcatactgct tttagaaggg aaaaattaaa
    12961 agggagagac tccgctctcc cattattggc tattttgcag ggttactgcg tggtaccgtc
    13021 ggttttggta tcgacatcat tattgatgcc atcaccggtt tgtacctttt tattgatatc
    13081 cgggcagcga ccatctttgc acatggtgtt cttgtgctct tcatctttgg tcatgccgtc
    13141 attgttcatt gaagaaccat ccggatgcag catagtgccg ccagaaccgg tatttacccc
    13201 gttattgtcg acgttatttg gcgcgacatt ttcacgggcg tcaggggcta cctggcccgc
    13261 atcagctgcg gcgtttgcct ggccgttatt ggtttgcgct ccgctatcgg cagccagtgc
    13321 ggcaccgctg gcaaggctta gagtggcagt cagaaatagt gtggccagtt ttgtcatttt
    13381 cataggatgc tcctgttatg gtcgttatgt cggataacct cttccaacag tgcatttgca
    13441 ggtgaatata aggcattggt ttaagatttc agccaggcaa agaaatccag cagagaatct
    13501 tgaaataatt aacaaacaaa ggagttacag ttagaaattg taggagagat ctcgtttttc
    13561 gcgacaatct ggcgtttttc ttgctaattc caggattaat ccgttcatag tgtaaaaccc
    13621 cgtttacaca ttctgacgga agatatagat tggaagtatt gcattcacta agataagtat
    13681 ggcaacactg gaacagacat gaattatcag aacgacgatt tacgcatcaa agaaatcaaa
    13741 gagttacttc ctcctgtcgc attgctggaa aaattccccg ctactgaaaa tgccgcgaat
    13801 acggttgccc atgcccgaaa agcgatccat aagatcctga aaggtaatga tgatcgcctg
    13861 ttggtggtga ttggcccgtg ctcaattcat gatcctgtcg cggctaaaga gtatgccact
    13921 cgcttgctgg cgctgcgtga agagctgaaa gatgagctgg aaatcgtaat gcgcgtctat
    13981 tttgaaaagc cgcgtactac ggtgggctgg aaagggctga ttaacgatcc gcatatggat
    14041 aacagcttcc agatcaacga cggtctgcgt atagcccgta aattgctgct tgatattaac
    14101 gacagcggtc tgccagcggc gggtgaattc ctcgatatga tcactcctca gtatctcgct
    14161 gacctgatga gctggggcgc aattggtgca cgtaccacgg aatcgcaggt gcaccgcgaa
    14221 ctggcgtctg gtctttcttg tccggtaggt tttaaaaatg gcacagacgg tacgattaaa
    14281 gtggctatcg atgccattaa tgccgccggt gcgccgcact gcttcctgtc cgtaactaaa
    14341 tgggggcatt cggcgattgt gaataccagc ggtaacggcg attgccatat cattctgcgc
    14401 ggcggtaaag agcctaacta cagcgcgaag cacgttgctg aagtgaaaga agggctgaac
    14461 aaagcaggtc tgccagctca ggtgatgatc gatttcagcc atgctaattc gtccaaacaa
    14521 ttcaaaaagc agatggatgt ttgtgctgac gtttgccagc agattgccgg tggcgaaaag
    14581 gccattatcg gtgtgatggt ggaaagtcat ctggtggaag gcaatcagag cctggagagc
    14641 ggggagccgc tggcctatgg taagagcatc accgatgcct gcattggctg ggaagatacc
    14701 gatgctctgt tacgtcaact ggcgaacgca gtgaaagcgc gtcgcgggta aagtttaatt
    14761 gtcggatgcg ccgtcagagt ggcgtatccg atgaatcacc gtagccctga caagacgcgc
    14821 agcgtcgcat caggcaatgt gctccattgt tagcgacgac aaacaaaaaa gccgactcac
    14881 ttgcagtcgg ctttctcatt tttaaacgaa tgacgtttac ttcgctttac cctggttcgc
    14941 aacggctgct gctttcgccg cgatctcgtc agcattaccc agataatagc gtttcagcgg
    15001 tttgaagttc tcgtcaaact cataaaccag cggcacgccg gtcgggatat tcagctcaag
    15061 aatctcttct tcgctcatgt tatcgagata tttcaccagc gcacgtaacg agttaccgtg
    15121 tgcagcgatg attacgcgct caccgctctt catacgcggc agaatggttt cattccagta
    15181 agggatcacg cggtcaatgg tcagcgccag gctttccgtc agcggcagtt ctttctcgct
    15241 cagtttcgcg taacgcggat cgtgacccgg ataacgctca tcatctttag tcagttccgg
    15301 cggagtcact gcaaaaccac gacgccactg tttcacctgc tcgtcgccat atttttcagc
    15361 agtttccgct ttgttcagac cctgcaacgc accgtagtga cgttcgttca gtttccagga
    15421 tttctcaacg ggcagccatg cctgatccag ttcgtccagc acattccaca gggtatggat
    15481 agcgcgtttc agcacagagg tgtaagcaaa gtcaaagctg taaccttcct ctttcagcag
    15541 cttacctgct gcttttgctt cgcttacgcc tttctcagac agatccacgt cgtaccaacc
    15601 ggtgaaacgg ttttctttgt tccactgact ttcgccatga cgaaccagaa ccagcttagt
    15661 tacagccata tacttactcc tcaaatcatc ttttaatgat aataattctc attatattgc
    15721 cgtgacgaag caacagcaat gcttacgcat aaccatagcg aaaatagtgg cgcagtgtaa
    15781 ggttgttgtg aatattgagt tgcaaatatg tcggtgtttg ctggtgattt gaacaatatg
    15841 agataaagcc ctcatgacga gggcttaaca ttactcagca ataaactgat attccgtcag
    15901 gctggaatac tcttcaccag gatgcaggaa acagtccggt tgcggccatt cagggtggtt
    15961 cgggctgtcc ggcagaaact cgctttccaa cgccagccct tgccagtcgg cgtaaggttc
    16021 ggttccccgc gacggtgtgc cgccgaggta gttgccggag tagaattgca gagccggagc
    16081 ggtggtgtag accttcagct gcaatttttc atctgctgac cagacatgcg ccgccacttt
    16141 cttgccatcg cctttggcct gtaacaagaa tgcgtgatcg taacctttca ctttgcgctg
    16201 atcgtcgtcg gcaagaaact cactggcgat gattttggcg ctgcggaaat caaaagacgt
    16261 tccggcgaca gatttcaggc cgtcgtgcgg aataccgcct tcatcaaccg gcagatattc
    16321 gtccgccaga atctgcaact tgtgattgcg cacgtcagac tgcttgccgt caagattgaa
    16381 atagacgtga ttagtcatat tcaccgggca aggtttatca actgtggcgc gataagtaat
    16441 ggagatacgg ttatcgtcgg tcagacgata ttgcaccgtc gcgccgagat tacccgggaa
    16501 gccctgatcg ccatcatctg aactcagggc aaacagcacc tgacgatcgt tctggttcac
    16561 aatctgccag cgacgtttgt cgaacccttc cgggccgccg tgcagctggt taacgccctg
    16621 acttggcgaa agcgtcacgg tttcaccgtc aaaggtataa cgactattgg cgatgcggtt
    16681 ggcataacga ccaatagagg cacccagaaa cgcggcctga tcctgatagc attccgggct
    16741 ggcacagccg agcagcgcct cgcggacgct gccatcggaa agcggaatac gggctgaaag
    16801 taaagtcgca ccccagtcca tcagcgtgac taccatccct gcgttgttac gcaaagttaa
    16861 cagtcggtac ggctgaccat cgggtgccag tgcgggagtt tcgttcagca ctgtcctgct
    16921 ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac ctgtttttgc ttcatattgt
    16981 tctgcgacag cttgctgtac ggcaggcacc agctcttccg ggatcagcgc gacgatacag
    17041 ccgccaaatc cgccgccggt catgcgtacg ccacctttgt cgccaatcac agctttgacg
    17101 atttctacca gagtgtcaat ttgcggcacg gtgatttcga aatcatcgcg catagaggca
    17161 tgagactccg ccatcaactc gcccatacgt ttcaggtcgc cttgctccag cgcgctggca
    17221 gcttcaacgg tgcgggcgtt ttcagtcagg atatgacgca cgcgtttcgc cacgattggg
    17281 tccagctcat gtgcaacagc gttgaactct tcaatggtga catcgcgcag agctggctgc
    17341 tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg tgttgtattc gctgccaacc
    17401 agggtacgtt tgaagttact gttgatgatg acgacagcca cacctttcgg catggaaact
    17461 gctttggtcc ccagtgagcg acagtcaatc agcaaggcat gatctttctt gccgagtgcg
    17521 gaaattagct gatccatgat cccgcagtta cagccaacaa actggttttc tgcttcctga
    17581 ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat aaagctgctg caatacggtt
    17641 ccgaccgcga cttccagtga agcggaagaa cttaacccgg caccctgcgg cacattgccg
    17701 ctgatcacca tgtccacacc gccgaagctg ttgttacgca gttgcagatg tttcaccacg
    17761 ccacgaacgt agttcgccca ttgatagttt tcatgcgcga caatgggcgc atcaagggaa
    17821 aactcatcaa gctgattttc ataatcggct gccattacgc gaactttacg gtcatcgcgt
    17881 ggcgcacagc tgatcacggt ttgataatca atcgcgcagg gcagaacgaa accgtcgttg
    17941 tagtcggtgt gttcaccaat caaattcacg cggccaggcg cctgaatggt atgagtggca
    18001 gggtagccaa atgcgttggc aaacagagat tgtgtttttt ctttcagact catttcttac
    18061 actccggatt cgcgaaaatg gatatcgctg actgcgcgca aacgctctgc tgcctgttct
    18121 gcggtcaggt ctcgctgggt ttctgccagc atttcataac caaccataaa tttacgtacg
    18181 gtggcggagc gcaacagagg cggataaaag tgcgcgtgca gctgccagtg ttgattctct
    18241 tcgccattaa acggtgcgcc gtgccagccc atagagtagg ggaaggagca ctggaagagg
    18301 ttgtcataac gactggtcag ctttttcaac gccagtgcca aatcgctgcg ctgggcgtcg
    18361 gtcaaatcgg tgatccgcaa aacgtgggct ttgggcagaa gtagcgtttc gaacggccag
    18421 gcagcccagt aaggcacaac tgctaaccag tgttcggttt cgacaacggt acggctaccg
    18481 tctgccagct cgcgctgaac ataatccacc agcattggcg atttctgctc ggcaaaatat
    18541 tctttttgca ggcggtcttc gcgctcagct tcgttaggca ggaagctatt tgcccaaatc
    18601 tgtccgtgcg gatgcgggtt agagcagccc atcgccgcac ctttgttttc aaagacctgc
    18661 acccacgggt atgttttccc cagttctgcg gtttgctcct gccaggtttt gacgatttcc
    18721 gtcaatgccg caacgctcag ttctggcagc gttttactgt gatccggtga aaagcagatc
    18781 acccggctgg taccgcgcgc gctctggcaa cgcatcaaag gatcgttgct ttctggcgca
    18841 tctggcgtgt cagacatcaa ggccgcaaag tcattagtga aaacgtaagt cccggtgtaa
    18901 tcggggtttt tatcgcctgt cacccgcaca ttacctgcgc agaggaagca atctggatcg
    18961 tgcgcaggta acacctgttt ggctggcgtt tcctgcgccc cctgccaggg gcgcttagcg
    19021 cggtgcggtg aaaccagaat ccattgcccg gtgagctggt tgtagcggcg atgtggatga
    19081 tcaacgggat taaattgcgt catggtcgtt ccttaatcgg gatatccctg tggatggcgt
    19141 gactgccagt gccaggtgtc ctgcgccatt tcatcgagtg tgcgcgttac gcgccagttc
    19201 agttcacggt cggctttgct ggcgtccgcc cagtaggccg gaaggtcgcc ctcgcgacgc
    19261 ggtgcaaaat gatagttaac cggtttgccg caggctttgc tgaaggcatt aaccacgtcc
    19321 agcacgctgc tgcctacgcc agcaccgagg ttgtagatgt gtacgcctgg cttgttcgcc
    19381 agtttttcca tcgccacgac gtgaccgtcc gccagatcca ttacgtggat gtaatcgcgt
    19441 acgccagtac cgtcttcggt cggataatcg ttaccaaaaa tcgccagcga gtcgcgacgg
    19501 cctacagcaa cctgggcgat gtatggcata aggttattcg gaatgccttg cggatcttcg
    19561 cccatatcgc ccgacggatg tgcgccaacc gggttgaagt agcgcagcag ggcaatgctc
    19621 cagtccggct gggctttttg cagatcggtg aggatctgtt ccaccatcag cttgcttttg
    19681 ccgtaagggc tttgcggtgt gccggtcggg aagctttcaa cgtatggaat tttgggctga
    19741 tcgccataaa cggtggcgga ggagctaaaa ataaagtttt tgacgttagc ggcgcgcatg
    19801 gcgctaatca ggcgcagagt accgttgaca ttgttgtcgt aatattccag cggtttttgt
    19861 accgattcgc caacggcttt cagcccggcg aagtggatca cggtgtcgat agcgtgatcg
    19921 tgcaggatct cggtcatcaa cgcttcgtta cggatatcgc cttcaacaaa cgtcggatgt
    19981 ttgccgccta aacgctcgat aacaggcagt acgctgcgct tactgttaca gaggttatca
    20041 agaatgatga catcatgacc gttttgcagt aattgcacac aggtatgact tccaatgtaa
    20101 ccgctaccac cagtgaccag aactctcata attcgctcca ttaggcttat ggtatgaaat
    20161 aaccatagca taacaaagat gcgaaaagtg tgacatggaa taaattagtg gaatcgttta
    20221 cacaagaatt tagccgtttt ttatgcgcga ttaagtgatt ataaaacaga gggattgtga
    20281 atgattgcgc tttttatctg aaaaaaagac gcagtttcat gcctggatgc gtctgaccgt
    20341 gtgccggata aagcgtttac gccgcatccg gcacagtcag cattaccgac ttagtttatt
    20401 tttgtcagcg cataatgata gaggccaccg tccggcacga actccagacg atgggtaata
    20461 caagccggcg catcttcagc atggtgcgaa acaaacaaca attgcgtttc accttcgcta
    20521 atcagcacat caacaaaacg gcgaataagc tggcgattga gcggatcaag cccctgcaat
    20581 ggttcgtcga gaataagcaa cgtcggatgt ttcaccagtg cgcggacgat cagcgccaga
    20641 cgctgctgtc cccaggaaag actatggaac ggagcgtcag ccgtgcgttt atcaatgccg
    20701 agaatatcca gccactgctg caccagtttt tgctggcgat ccgaaacggc ctgataaatg
    20761 ccaatcgaat caaaatagcc agaaagaatc acattacgca cagtagtact gacccggtaa
    20821 tccagatgca aactactgct gacgtaaccg atatgctttt tgatatccca gatggtttcg
    20881 ccgctgccac gacgtcgtcc gaaaagcgtc aaatcgttgc tgtaaccttg cggatgatcg
    20941 ccagtaatca ggcttaataa cgtcgatttt cccgcgccat ttggcccgac aatttgccag
    21001 tgttcacctg gattcacctg ccagctaagg ttattaagaa tggggcgatc gttataagaa
    21061 accacgccat tattcagcac aatgcgcggt tcgttggcgg gtaaggcgtg acgtgctgaa
    21121 ggttcatccg gctccggcag ttgcacacct tcaagctgtt cactatgcgc cagttgcgcg
    21181 acgagtgctt gttggagcag ttcctcttta gcgccagttt ccgctaacgt gcaatccgcc
    21241 agcacgccag caaactggac aaactccggg atctcatcga agcgattgag caccagtacc
    21301 agagtaatac cggactgatg taacgaggcg agtcgctcag ccagctgctg acgtgaggca
    21361 acatccaggc catcgaacgg ctcatcaaga atcaacaagt caggctccga catcagcgcc
    21421 tgacacagca gggtttttcg cgtctcgcca gtggaaaggt atttaaagcg tcggtcgagg
    21481 aggggggtaa taccgaactg ctgtgccagt tgcatgcagc gcggtgcatc ctttacttca
    21541 tcctgaataa tctcagccgt agtgcgtccg gtgtcatctt cgccagggcc gagcatatcg
    21601 gtgttattcc gctgccattc gtcgctgacg agcttttgca attgctcgaa ggagagacga
    21661 gtgatgtggg aaaactggct ttgccgttca cctttcaaaa gcggaagttc ccccgccagc
    21721 gcgcgggcca gggccgattt cccgcttcca ttcgaaccga caaacgccca actatcaccc
    21781 gcgtttaacg ttagctgagg caattgcagc gtttttgtgt cgctaagacg aaacgtgcct
    21841 tgcaaaattt gcaacgatga cattttatat cccattttgt gcagcgatta ctgacaggga
    21901 tacgtgtttc ataacaaatt gtcaacacgc ttagcacagt gtggcgataa tcacgctgtc
    21961 ggcattaaag taggccgtga cattctgtcc ttgctgaaga gaagtcgctt catttaccgg
    22021 cactgtggcg cacagtgttt gcccgtccgg tagcgccatt aatacttcgc actgctctgc
    22081 gccgcgctca atatgactaa taatacccgg taattggttg tcagcgtttt gcgcgaccgc
    22141 ttcgtcctga gtaatgccta cccacggcgc ttttagcaat atcaacactt ctttgccttc
    22201 atccagcccc agacgcgcgc cgctttgtgc ggtaattgcg actttcaggc gtgtttttcc
    22261 atcagccagt aagacatcaa catgctgttg aacgtcatca tgatcgcggg cggtgatggt
    22321 accgaaccac tggttacggg cgctggtttg gagtgaaaaa cgtgagatcg cggccagcag
    22381 gctgttcagc ggcagggcgt catcgtcact taacacatca aaggcttttt gctggatttg
    22441 cgccagtaag tcatagagct gaatcagtcg ctgaccatag cgggtcagta ctgcgccgcc
    22501 gccaccttta ccgcctgttg cgcgctcgac cagaatatgc tcacttaact gattcatctc
    22561 gttaatggca tcccaggcgc ttttatagct gataccggca tctttcgctc cctggctaat
    22621 ggaaccggaa agcgcaatgt gttttagtag cgaaatgcgg cgcgggtcgg cgaataattt
    22681 ttgttggagc ttaagggtga gaaggatttc ggcctgcata acaatgtcct ggcaaaagtc
    22741 ttattgtgac ggaaaacgaa cgccacgcaa agctgaccgc acaaaagggg agtgcttttc
    22801 tgtgcttagc ggttagaata gtctcatgac tatatctgga gttgaccatg ttagagttat
    22861 taaaaagtct ggtattcgcc gtaatcatgg tacctgtcgt gatggccatc atcctgggtc
    22921 tgatttacgg tcttggtgaa gtattcaaca tcttttctgg tgttggtaaa aaagaccagc
    22981 ccggacaaaa tcattgattc cctgaatgcc cgcttagtcg ggcattttct ttttctcaac
    23041 ttcctgcttt tcctgccgat attttttctt atctacctca caaaggttag caataactgc
    23101 tgggaaaatt ccgagttagt cgttatattg tcgcctacat aacgttacat taaggggtta
    23161 ccaatggctc gtaaatggtt gaacttgttt gctggggcgg cactctcttt cgctgttgct
    23221 ggcaatgcac tggcagatga agggaaaatc acggtgttcg ccgccgcatc actgactaac
    23281 gcaatgcagg acattgctac gcagtataaa aaagagaaag gcgtggatgt ggtttcttct
    23341 ttcgcttcgt catctactct cgcccgtcag attgaagcgg gtgcccctgc ggatctgttt
    23401 atttctgccg atcagaaatg gatggattat gcggttgata aaaaagcgat cgatacagct
    23461 acgcgtcaga cactgctcgg caatagcctg gtcgttgtag caccgaaagc cagcgagcag
    23521 aaagatttta ccatcgacag caaaaccaac tggacttcac tgctgaatgg cggtcgcctg
    23581 gcggttggcg atccggaaca tgttcccgct ggcatttatg caaaagaagc actgcaaaaa
    23641 ctgggcgcat gggatacgct ctccccgaaa ctggctccgg cggaagatgt tcgtggcgcg
    23701 ctggcgttgg tcgaacgcaa tgaagcgccg ttgggcattg tttacggttc tgacgcagtt
    23761 gctagcaaag gggtaaaagt ggttgccacc ttcccggaag attcacataa aaaagtggaa
    23821 tatccggttg ctgttgtgga agggcataac aatgcgacag tgaaagcatt ttatgattat
    23881 ctgaagggac cgcaggcagc ggaaatcttt aaacgttacg gatttacaac caagtaatga
    23941 tactgaccga tccagaatgg caggcagttt tattaagcct gaaagtttct tccctggctg
    24001 tgctgtttag cctgccgttt gggatctttt ttgcctggtt actggtgcgt tgcacgtttc
    24061 cgggcaaagc tctgctcgac agcgtactgc atctaccgct ggtgttaccg cccgtggtcg
    24121 tcggttactt attattagtt tcgatggggc ggcgcggatt tatcggtgaa cgtctgtatg
    24181 actggtttgg tattaccttc gcctttagct ggcgcggcgc ggttctcgct gccgccgtca
    24241 tgtcgtttcc gctgatggtg cgggcaattc gtctggcgct ggaaggggtt gatgtcaaac
    24301 tggaacaggc cgcaagaaca ctgggggccg ggcgctggcg cgttttcttt actatcacgt
    24361 taccgctgac cttaccggga attattgttg gtacggtact ggcttttgct cgttctctcg
    24421 gtgaatttgg cgcaaccatc acctttgtgt cgaacattcc tggtgaaacg cgaaccattc
    24481 cttctgccat gtataccctg attcagaccc ccggcgggga aagtggcgca gcgagactgt
    24541 gcattatttc tattgcgctg gcgatgatct ccctgttgat ttcagaatgg ctggccagaa
    24601 tcagccgtga acgggcgggg cgctaatcat gctggaactg aatttttccc agacgttggg
    24661 caaccattgc ctgactatta atgaaacgct gcccgccaat ggcatcactg ctatctttgg
    24721 cgtctccggt gccggaaaaa cttcgctgat taacgccatc agtggactga cgcgcccgca
    24781 aaaagggagg attgtcctca atggtcgggt actaaatgat gccgaaaaag gtatctgcct
    24841 gacgccggaa aagcgtcgcg ttggctatgt ttttcaggat gcgcggctgt tcccgcatta
    24901 caaagtgcgt ggcaatctgc gctacggcat ggcgaaaagc atggtcgatc agttcgataa
    24961 gctggtggcg cttttaggca ttgaaccgtt gcttgaccgt ttaccaggca gcctgtccgg
    25021 aggcgaaaaa cagcgcgtag cgattgggcg ggctttgctg acggcaccag aattgctgtt
    25081 gctggatgaa ccgctggcgt cactggatat tccgcgtaaa cgcgaactgt tgccttatct
    25141 gcaacgtctg acgcgggaaa tcaacatccc gatgttgtat gtcagccatt cgctggatga
    25201 gatcctccat ctggcagaca gagtgatggt actggaaaac ggtcaggtga aagcctttgg
    25261 cgcgctggaa gaagtctggg gtagtagcgt gatgaatccg tggctgccga aagagcaaca
    25321 aagtagcatt ctgaaagtga cggtacttga gcatcatccg cattacgcga tgaccgcgct
    25381 ggcgctgggc gatcagcatt tgtgggtcaa taagctggac gaaccgctgc aagctgcgct
    25441 acgtatccgc attcaggctt ccgatgtttc tctggtttta caaccgccgc agcaaaccag
    25501 cattcgtaac gtattgcggg caaaagttgt taatagttat gacgacaacg gccaggtgga
    25561 agtggaactg gaagtcggcg gtaaaacgct gtgggcgcgt atcagcccgt gggccaggga
    25621 tgaactggcg atcaaacctg gcctgtggct gtacgcgcaa attaaaagtg tgtcgataac
    25681 cgcctgatta aatcaggtgg ctataaatga actgggcaat gctgtcggtg gtgttatcac
    25741 caatcacaat gttggcgcgc gcttttaccg cgtcatcggc gttgcccatc gccacgcctg
    25801 taccagcggc ctccagcata ctgatatcat taaagttatc gccgaacgcc acgacgtttt
    25861 ccatcgacca gccttgcgcc tcaacccatt tcgtcaaacg tttacctttg ctgttgccgc
    25921 cgcgtgcaat atcaacctga tcgtgccagg accattcaca ctccagtccc agttcatgtt
    25981 cgacatgctt accaaaatgc tgcaattgcg gcaggtcatc gtgcgtgagg gcgaacttcc
    26041 atacggcgtt aacttgttgc gccgtttcag ccagagaagc gacttgtgtg aaagtcggac
    26101 gctgttccgg cggcagggtt tgcgcccagt tagacgtgcg aatgacatgc ccggtcgggt
    26161 gctcataaac catctcgtca tcgacataca tcagaccgtg aatgtggtgt tcattcagca
    26221 tctcaatgag ttgtagggct ttattaacgg gcattgggtc cgcttccagc acggtttttg
    26281 catgataatc atacaaatag gtgccattac agcaaatagc aggtgtatcc agcgccagcg
    26341 cctgataaaa aggatgaata gcgacgtgat ggcgacctgt gacgatgatt aattgataac
    26401 ctgcttcgcg agcgcgggcc agggcttcta tcgatgaagg tagcagggtc tttttcgggg
    26461 tcaataaggt gccgtctaag tcgagagcaa tcacgcgtgt ggtcatggcg tattccagat
    26521 taaggttaag aattttcttc tgcgcgaatg gtacaccgat accactatcg gacaaaattc
    26581 tgcgttttaa ttcagcattc accgccaaaa gcgactaatt ttagctgtta cagtcagttg
    26641 ctaaatgcaa aggagcattc atgaagcaaa cagtttatat cgccagccct gagagccagc
    26701 aaattcacgt ctggaacctg aatcatgaag gcgcactgac gctgacacag gttgtcgatg
    26761 tgccggggca ggtgcagccg atggtggtca gcccggacaa acgttatctc tatgttggtg
    26821 ttcgccctga gtttcgcgtc ctggcgtatc gtatcgcccc ggacgatggc gcactgacct
    26881 ttgccgcaga gtctgcgctg ccgggtagtc cgacgcatat ttccaccgat caccaggggc
    26941 agtttgtctt tgtaggttct tacaatgcgg gtaacgtgag cgtaacgcgt ctggaagatg
    27001 gcctgccagt tggcgtcgtc gatgtggtcg aggggctgga cggttgccat tccgccaata
    27061 tctcaccgga caaccgtacg ctgtgggttc cggcattaaa gcaggatcgc atttgcctgt
    27121 ttacggtcag cgatgatggt catctggtgg cgcaggaccc tgcggaagtg actaccgttg
    27181 aaggggccgg cccgcgtcat atggtattcc atccaaacga acaatatgca tattgcgtca
    27241 atgagttaaa cagctcagtg gatgtctggg aactgaaaga tccgcacggc aatatcgaat
    27301 gtgtccagac gctggatatg atgccggaaa acttctccga cacccgttgg gcggctgata
    27361 ttcatattac cccggatggt cgtcacttat atgcctgtga ccgtaccgcc agcctgatta
    27421 ctgtcttcag cgtttcggaa gatggcagcg tgttgagtaa agagggcttc cagccaactg
    27481 aaacccagcc gcgcggcttt aatatcgatc accgtggtaa gtatctgatt gccgccgggc
    27541 aaaaatcgca ccacatctcg gtatacgaaa ttgttggtga gcaggggcta ctgcatgaaa
    27601 aaggccgcta cgcggtcggg cagggaccaa tgtgggtggt ggttaacgca cactaaccgc
    27661 tgatttaccc ggcgcagtct ctcctgcgcc ggtgtattaa cctatctcct gtaacgcatg
    27721 tctctggcgt tcgacgatat tggtccacaa attgtcttta tcgtcagtcc acaaattaat
    27781 cagcaaggca aaaaagcgtt ctgctgccgg agaaagcacg gcatctttgc ggcgaataat
    27841 ccccaatgtc cgacgtataa cgggttccac cagtgggata ccaataatag aagaataggg
    27901 cgcatggggc attgccagac caggaagcgc cgaaattccc agtcccgcct ctaccagtcc
    27961 taatgacgtc gaaagatgac gcacttcgta aaaccagtcc agcttccagg gcttgtcggc
    28021 tagctgttgt tctatcaaca atcggttgcc actggaggaa cgtacgccaa tcattttgta
    28081 acccactaac tcctgccatt ctacaagctg ctttttagcc aatggatggt cacgccgaca
    28141 ggccagcacg aacggctcgt tgaccagtgg ggtaaaatca atggatgaat ttgtgacgtt
    28201 gttcatgttt atgccaaagt ctgattcgtt acacagcacc gattccatgc aattattcgt
    28261 gccttgttcc agaatccgca ctttaatatt gggatacagc tcattaaatt taccgattgc
    28321 cagcggtaaa aaataaaata ctgcggtcgg aatacacgct aacgtcacca taccacgatg
    28381 ataagcattc atatcacgaa tattaaaaag cgtttcatcg aatttcttta ttaattcccg
    28441 cgcctctggg agcaaccttt ttcctgcttt tgttaacgta accttgcgag ttgtacgctc
    28501 aaatagttga atatgtaaat cttcctccat cttttttatt cttcgtgtta atgcaggttg
    28561 cgtaatattg agtaatttag cggcattgtt aaatgaactg gactctgcca gtatgacaaa
    28621 tgccttcata cttgataatt catgcttcat tattactccg ggaaatggaa gcgacgattt
    28681 tgggtggctg gccgttaaaa attttaactg catttagcca acttaaatta atgaaaaaat
    28741 gttattaatc gttgagctaa agtcattaga gatgctttgc ccttaatgta accatatcgc
    28801 aataagttat gtttttaaat tgagggcatt attatgaaaa aaataccctg cgtgatgatg
    28861 cgaggtggaa cctcgagggg cgcgttcctg ttagcggaac atttacccga agatcaaacg
    28921 cagcgcgata aaatattgat ggcaattatg ggttccggta acgatctgga aattgacggt
    28981 attggcggcg gtaatccact gaccagtaaa gtcgccatta ttagccgttc cagcgatccg
    29041 cgtgctgatg ttgattatct gtttgctcaa gttatcgtcc atgagaaacg tgtcgatacc
    29101 acgcctaact gcggcaatat gctgtctggc gttggggcat tcgccattga aaatggtttg
    29161 attgcagcga cttcgccagt tacccgcgta cgtatccgca acgtcaatac gggtacgttc
    29221 atcgaagctg atgtgcaaac gccaaatggt gttgtcgagt acgagggtag cgccagaatt
    29281 gacggcgtac cgggtactgc cgcaccggtt gcgctcactt tcctgaatgc cgctggaacg
    29341 aaaaccggaa aagttttccc gactgataat cagattgatt attttgacga tgtcccggtg
    29401 acctgtatcg atatggcgat gccagtcgtc attattccgg ctgaatatct gggaaaaaca
    29461 ggctatgaat taccggcgga actggatgcc gacaaagcat tattagcccg cattgaatct
    29521 atccgtctac aagcgggtaa agcaatgggc ttaggcgatg tcagtaatat ggttatccct
    29581 aaacctgtgc ttatttctcc agcgcagaaa ggcggggcaa ttaatgtgcg ttattttatg
    29641 ccgcattctt gccatcgcgc gctggcgata accggtgcta ttgctatttc cagtagttgt
    29701 gcattggaag gtaccgtcac ccgacaaatc gtcccttctg taggatacgg caatatcaat
    29761 attgaacacc ccagtggtgg gctcgacgtt catttaagta atgaaggtca ggatgccacg
    29821 acgttacgcg catctgttat tcggacaacc agaaaaatat tttccggtga agtttatctt
    29881 ccctgaaaag attcgttgtc aggataagga caatcaataa aggacttctg tatgagtcat
    29941 acagaaagaa caggatttta aatgaataag aaatcgttat ggaagctaat tctgatatta
    30001 gcgatcccat gtattattgg ctttatgcca gctccggcag gattaagcga actggcgtgg
    30061 gtgctttttg gtatttacct ggcggccatt gtggggctgg ttatcaagcc tttcccggaa
    30121 cctgtcgtac tgttaattgc cgttgctgcc tccatggtgg tggtcggtaa cttatccgac
    30181 ggtgcgttta agaccaccgc cgtattaagc ggttactctt caggtactac ctggctggtg
    30241 ttctcggcgt ttaccttaag cgccgcattt gtgaccaccg gtttaggtaa acgtattgcc
    30301 tatctgctga ttggtaaaat cggtaacacc acgctgggtc tgggttacgt tacggtattc
    30361 ctcgatctgg tattggctcc ggcaacaccg tctaacaccg cgcgtgcggg cggcattgtg
    30421 ttaccgatca tcaacagcgt ggcggtggct ttggggtccg aaccggaaaa aagtccgcgt
    30481 cgtgtcggac attacctgat gatgtccatt tacatggtca ccaaaaccac cagctatatg
    30541 ttctttaccg caatggcggg gaacattctg gcgctgaaaa tgatcaacga cattctgcac
    30601 ctgcaaatta gctggggtgg atgggcgctg gccgccggat tgccgggcat cattatgctg
    30661 ctggtcaccc cgctggtgat ttacaccatg tatccaccag aaattaagaa ggtggataac
    30721 aaaaccatcg ctaaagcggg ccttgccgaa ctgggaccga tgaaaatccg cgaaaaaatg
    30781 ctgctcggtg tctttgtgct ggcgctgctg ggctggattt tcagtaagtc tctgggggtt
    30841 gatgaatctt cagtggctat tgttgttatg gcgaccatgc tgctgctggg tatcgttacc
    30901 tgggaagacg tggttaaaaa taaaggcggc tggaatacct taatctggta cggcggtatt
    30961 atcggcttaa gctccttatt atcgaaagtt aaatttttcg aatggttagc tgaagtcttt
    31021 aaaaataacc tggcatttga tggtcacggt aacgttgctt tcttcgttat tattttcctc
    31081 agcatcatcg tgcgttattt cttcgcttcc ggtagtgcct atatcgttgc tatgttaccg
    31141 gtatttgcca tgctggcgaa cgtctccggc gcaccgttaa tgttaaccgc gctggcactg
    31201 ttgttctcca actcctatgg cggcatggtt actcactatg gcggcgcggc aggtccggtc
    31261 atctttggcg tgggttataa cgatattaaa tcctggtggt tggtcggtgc ggtactgacg
    31321 atattaacct tcctggtgca tatcaccctc ggcgtgtggt ggtggaatat gctgatcggc
    31381 tggaacatgc tgtaaatata ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg
    31441 ctcaccccag tcacttactt atgtaagctc ctggggattc actcgcttgt cgccttcctg
    31501 caactcgaat tatttagagt atatccattt attttcttgc tgcgcacttc acggtgcgca
    31561 gatatctgga gcatttgatg atcaagttat ctgaaaaagg cgtgtttctc gccagtaata
    31621 acgaaataat tgccgaagaa catttcaccg gcgaaattaa aaaagaagaa gcaaaaaaag
    31681 gcactattgc ctggtctatt ctctcttctc ataatacgtc cggaaatatg gataaactta
    31741 aaattaagtt tgattcatta gcctctcacg atattacctt tgttggtatt gtacagaccg
    31801 ctaaagcgtc cggtatggaa cgtttcccgc tgccgtatgt gctgaccaac tgccataact
    31861 cactctgcgc cgtcggcggc accattaacg gtgatgacca tgtttttggt ttatcggcag
    31921 ctcagcgtta tggcggtatt tttgtgcctc cgcatattgc ggtcatccat caatatatgc
    31981 gtgagatgat ggcaggtggc ggcaaaatga tcctcgggtc agacagtcac acccgttacg
    32041 gtgcattagg gacaatggca gtcggtgagg gcggcggcga gttggtaaaa caattgctta
    32101 atgacacctg ggatatcgac tatccgggag ttgttgcggt gcatctgacc ggaaaaccag
    32161 cgccgtatgt ggggccgcag gatgtggcgt tggctatcat cggtgccgtg ttcaaaaacg
    32221 gctacgtcaa aaacaaagtg atggaattcg taggtcccgg tgttgctgcg ctctctaccg
    32281 atttccgtaa cagcgttgac gttatgacca ctgaaacgac ctgtttaagt tctgtctggc
    32341 aaaccgatga agaagtccat aactggctgg cgctgcacgg tcgcggccag gattactgcc
    32401 agcttaaccc tcaaccgatg gcgtactacg atggctgcat cagcgttgat ttaagcgcca
    32461 tcaaaccaat gattgcgctg ccgttccacc cgagcaacgt gtatgaaatc gacacactga
    32521 accagaactt gaccgacatt ctgcgtgaga ttgaaattga gtccgaacgc gtggcgcacg
    32581 gtaaagccaa actctcgctg ctggacaaag tggaaaatgg tcgcctgaaa gtgcagcagg
    32641 ggattatcgc gggctgttct ggcggtaact acgaaaacgt catcgcggcg gcgaatgcac
    32701 tgcgcggtca atcctgtggc aatgacacct tctcgctggc ggtttacccg tcatcacagc
    32761 cggtgtttat ggatctcgcc aaaaaaggtg tggtagcaga tttgattggc gcaggcgcaa
    32821 tcatcagaac cgcgttctgc ggcccatgct ttggcgcggg cgatacgcca atcaacaacg
    32881 gtttgagtat tcgccacacc acgcgtaact tcccgaaccg cgaaggctct aagccagcta
    32941 atgggcagat gtcagcggtg gcgttgatgg acgctcgttc tatcgctgcg actgcggcaa
    33001 acggtggcta tttaacctct gccagcgaac tcgattgctg ggacaacgtg ccggagtacg
    33061 ccttcgatgt aacgccgtat aaaaatcgcg tctatcaagg ctttgtgaaa ggggcggccc
    33121 agcaaccgct gatttacgga ccgaacatta aagactggcc ggaattgggt gcgctgactg
    33181 acaatatcgt cctcaaagtg tgctcgaaga tcctcgacga agtgaccacc accgacgaat
    33241 tgattccttc cggtgaaacc tcttcttatc gttcaaatcc gattggtctg gcggagttta
    33301 ccctgtcacg ccgcgatcca ggttatgttg gcagcagtaa agcgactgct gagctggaaa
    33361 atcagcgtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga
    33421 ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg gtctatgcgg
    33481 tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ttgtcagcgt gtgattggcg
    33541 gtctggcgaa tattgccgag gagtacgcga ctaaacgcta tcgttctaac gtcatcaact
    33601 gggggatgtt accgctgcag atggtggaag cgccaacctt tgaagtgggg gattacattt
    33661 acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa ggttatgtga
    33721 tccatgaaga tgcgccggta acggaaatta cgctctatat ggaaagtctg actgctgaag
    33781 agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa
    33841 aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat gctggcgcat
    33901 cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta cgccgcatcc
    33961 ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgcta cccacgccgc
    34021 ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt
    34081 cgctatcgtc aacgctgccg gtgttacccg caaacggacg attagagatc accgcatcgc
    34141 cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac
    34201 cgttggtatt ggcatcaaca tccagcgagc ggcccagttg tgccacgcca tcaccggaag
    34261 cattgaaacg gctgtttacg gcgaggaaac cgtagtaaat gttggacagc gtagccggtg
    34321 caaacacata cgcttcttgc tgagtacgtg agttcaccac gcggaattcg gtgttatcga
    34381 acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatgtag ctgttggtca
    34441 ccagcgtacg cggctgacga ttcgtttcca gacggttctg cacaccgctg ttggtgacaa
    34501 agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg tcaccgtcag
    34561 tacgcagtgc caccgccgga tggttacccg catctacgct atcacccagc gtgttttcga
    34621 tggtcaggtt ttgcagttgc aggccattgt tttgtgacca gaagaccgca gaacacagaa
    34681 caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac
    34741 ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc
    34801 catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtaa
    34861 ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc atcacggcaa
    34921 tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg
    34981 tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg
    35041 ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgag
    35101 catcgaaatt ctgcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcag
    35161 tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa
    35221 aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat
    35281 gaaatagaaa aatgaatccg ttgaagtctg cttttttata ctaagttggc attataaaaa
    35341 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
    35401 tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg atgccatggt
    35461 gtccgactta tgcccgagaa gatgttgagc aaacttatcg cttatctgct tctcatagag
    35521 tcttgcagac aaactgcgca actcgtgaaa ggtaggcgga tccccttcga aggaaagacc
    35581 tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa gcggttcacg
    35641 acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat caagtgtttc
    35701 cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa tttttacgcc
    35761 tgttttgctt tgttcgacat aaagatatcc atctacgata tcagaccact tcatttcgca
    35821 taaatcacca actcgctgcc cggtaacaac agccagttcc attgcaagtc tgagccaaca
    35881 tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa gtcttgatct
    35941 ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta tatggccttc
    36001 agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg ctgacgccgc
    36061 cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg tgatgtcttc
    36121 aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt aatttatgag
    36181 tgtcttctgc ttgattcctc tgctggcgag gattttttcg tagcgatcaa gccatgaatg
    36241 taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt tgtgtcctga
    36301 aaataactca atattggcct gtattgcttc agtgattgct atcctcctgt ctcggcctaa
    36361 accaaactct ttacccgtcc ttgggtccct gtagcagtaa tattgtttct tatataaagg
    36421 ttagggggta aatcccggcg ctcatgactt cgccttcttc ccatttctga tcctcttcaa
    36481 aaggctacct gttactggtc gatttaagtc aacctttacc gctgattcgt ggaacagata
    36541 ctctcttcca tccttaaccg gaggagggaa tatcctgcat tcgcgcaccc atcgacgaac
    36601 tgtttcaagg cttcttgggc gtcgctggcg tgcgttccac tcctgaagtg tcaagtacat
    36661 cgcaaagtct ccgcaattac acgcaagaaa aagccgccat caggcggctt ggtgttcttt
    36721 cagttcttca attcgaatat tggttatgtc tgcatgtgct atctgcgccc atatcatcca
    36781 gtggtcatag cagtcattga tgttctctgc ttcgataact ctgttgaatg gttctccatt
    36841 ccattcacct gtgactcgga agtgcattta tcatctccat aaaacaaaac ccgccgtagc
    36901 gagttcagat aaaagaaatc ctcgtcagtg cgaggatgct gttcattgct gctatacact
    36961 tttttgctct caacgtaagc ggtagctcat tctgttgggt tggtgcagtt gcttttagga
    37021 aatgctattt accccttaaa cgtcggctga aagagctaaa atccatgcaa aaaatttacg
    37081 caattttgtg tattattgtg cagtaagtaa tgagctattt tctgcgcaaa aaatggatgg
    37141 taaatttgtc cggggcagga aaaattttat gggcgctaaa catgaaaaaa gattcgtatc
    37201 cttatttgat ttgcatgaca gttttaggcc tgatctttat tttccttttc ttctggtggc
    37261 gggcagatat ttacagggtc acgtttctta atcagagtat atcccactat tacattctgt
    37321 ttagcatggg aatagctttt ctgttatctc tgttttgggt taagaagggg atagtaaaac
    37381 aaagcggctg gaagagtctg tcagcatacc ttaaggttta tgcagggatg tgcatatttg
    37441 ctggattttt tctgattata ccccttacaa cactaactta ttttttgcct ggagagacat
    37501 cgtcttatgt tgcaccgtat cggtatactt ccggtagttc aaaaagttgt tctggagctg
    37561 aggtggatga ccccgatcta catgagaata ttcgcatttg ctatccgtat ggcaattatg
    37621 agtacgataa tattatctat gttgaaaaga aaattaatac attaggtgcg gtagtaacat
    37681 atgcacagac cgcgcgtgat gatactgaat gagatagtat atagcgggca agttttagtt
    37741 aatttatcga ggtaatataa tttacctcga ctcgtttatt ctggtattaa tatttcgctt
    37801 tacgatcgat ttttatctga tgatatcatg cggttttcat atactgactt actgtctttt
    37861 ctccgttagc gattttctcc tgctcagcga tgattttatc tttggcttct agttaatttc
    37921 gctcacttcg aacctctctg tttactgata agttccagat cctcctggca acttgcacaa
    37981 gtccgacaac cctgaacggc caggcgtctt cgctcatcta tgggatcgcc acactcacaa
    38041 caatgagtgg cagatatagc ctggtggttc aggcggcgca tttttattgc tgtgttgcgc
    38101 tgtaattctt caatttctga tgctgaatca atgatgtctg ccatcttcca ttaatccctg
    38161 aattgttggt taatacgctt gagggtgaat gcgaataata aaaaaggagc ctgtagctcc
    38221 ctgatgattt tgcttttcat gttcaccgtt ccttaaagac gccgtttaac atgccgattg
    38281 ccagacttaa gtgagtcggt gtgaatccca tcagcgttac cgtttcgcgg tgcttcttca
    38341 gtacgctacg gcaaatgtca tcgacgtttt tatccggaaa cggctgtctg gcttttttga
    38401 tttcagaatt agcctgacgg gcaatactgc gaagggcgtt ttcttgctga ggtgtcattg
    38461 aacaagtccc atgtcggcaa gcataagcac acagaatatg aagcccgctg ccagaaaaat
    38521 gcattcagtg gttgtcatac caggtctctc tcatctgctt ctgctttcgc caccatcatt
    38581 tccagctttt gcgaaaggga tgtggctaac gtatgaaatt cttcgtctgt ttctactggt
    38641 attggcacaa acctgactcc aatttgagca aggctatgtg ccatctcaat actcgttctt
    38701 aactcaacag gagatgcttt gtgcatatcg cctcccgttt attatttatc tcctcagcca
    38761 gccgctgggc tttcagcgga tttcggataa cagaaaggcc gggaaatacc cagcctcgct
    38821 tcgtaacgga gtagacgaaa gtgatcgtgc ctacgcggat attatcgtga ggatgcttca
    38881 tcgccattgc tccccaaata caaaaccaat ttcagccagt gcctcgtcca ttttttcgat
    38941 gaactccggc accatctcgt caaaacccgc catgtacttt tcatcccgct caaccacgac
    39001 ataatgcagg ccttcacgct tcatacgcgg gtcatagttg gcaaagtacc aggcatcttt
    39061 tcgcgtcacc cacatgctgt actgcacctg ggccatgtaa gccgacttta tggcctcgaa
    39121 accaccgagc ctgaacttca tgaaatcccg ggaggtaaac gggcatttca gctcaaggcc
    39181 gttgccgtca ctgcataaac catcgggaga gcaggcggtg cgcatacttt cgtcgcgata
    39241 gatgatcggg gattcagtaa cattcacgcc ggaagtgaat tcaaacaggg ttctggcgtc
    39301 gttctcgtac tgttttcccc aggccagcgc cttagcatta acttccggag ccacaccggt
    39361 gcaaacctca gccagcaggg tgtggaagta ggacattttc atgtcaggcc acttctttcc
    39421 tgagcggggc tttgctatca cgttgtgaac ttctgaagcg gtgatgacgc cgagccgtaa
    39481 tttgtgccat gcatcatccc cctgttcgac agctctcacg tcgatcccgg tacgctgcag
    39541 gataatgtcc ggtgtcatgc tgccaccttc tgctcagtgg ctttctgttt caggaatcca
    39601 agagctttca ctgcttcggc ctgtgtcagt tctgacgatg cgcgaatgtc gcggcgaaat
    39661 atctgggaac agagcggcaa taagtcgtca tcccatgttt tatccagggc gattagcaga
    39721 gtgttaatct cctgcatggt ttcatcgtta accggagtga tgtcgcgttc cggctggcgt
    39781 tctgcagtgt atgcagtatt ttcgacaatg cgctcggctt catccttgtc atagatacca
    39841 gcaaatccga aggccagacg ggcacactga atcatagctt tatgccgtaa catccgtttg
    39901 ggatgcgact gccacggccc cgtgatttct ctgccttcgc gggttttgaa tggttcgcgg
    39961 cggcattcat ccatccattc ggtaacgcag atcggatgat tacggtcctt gcggtaaatc
    40021 cggcatgtac aggattcatt gtcctgctca aagtccatgc catcaaactg ctggttttca
    40081 ttgatgatgc gggaccagcc atcaacgccc accaccggaa cgatgccgtt ctgcttatca
    40141 gggaaggcgt aaatttcttt cgtccacgga ttaaggccgt actggttggc gacgatcaac
    40201 aatgcgatga actgcgcatc gctggcatga aagcgcgatg gggagacagg gcggcatgag
    40261 acgacagcga cgaagtttca ccgacatcat ctgcgaaaac tgcaaatacc ttccaacgaa
    40321 acgctccaga aataaacgca agccaatccc aaaagaatct gacgtaaaaa ccttcaacta
    40381 cacggctcac ctgtgggata tccggtggct aagacattgt gcgaggaaaa caaggtgatt
    40441 gaccaaaatc gaagttacga acaagaaagc gtcgagcggg ctttaacgtg cgctaactgc
    40501 ggtcagaagc tgcatgtgct ggaagttcac gtgtgtgagc actgctgcgc agagctgatg
    40561 agcgattcga atagctcgat gcacgaggaa aaagatgatg gctaaaccag cgcgaagacg
    40621 atgtaaaaac gatgaatgtc gggaatggtt tcaccctgca ttcgctaatc agtggtggtg
    40681 ctctccagag tgtggaacca agatagcact cgaacgacga agcaaagaac gcgaaaaagc
    40741 ggaaaaggca gaaaaggcag cagagaagaa acgacgacga gaggagcaga aacagaaaga
    40801 taaacttaag attcaaaaac tcgccttaaa gccccgcagt tactggatta aacaagccca
    40861 acaagccgta cacgccttca tcagagaaag agaccgcgac ttaccatgta tctcgtgcgg
    40921 aacgctcacg tctgctcagt gggatgccgg acattaccgg acaactgctg cggcacctca
    40981 actccgattt gatgaacgca atattcacaa gcaatgcgtg gtgtgcaacc agcacaaaag
    41041 cggaaatctc gttccgtatc gcgtcgaact gattagccgc atcgggcagg aagcagtaga
    41101 ggaaatcgaa tcaaaccata accgctatcg ctggactgtc gaagagtgca gggccatcaa
    41161 ggcggagtat caacagaaac ttaaaaaact gcgaaacagc agaagtgagg ttgcatgaat
    41221 atctacgaaa gaattgatgg cagcaaatac cgaaatattt gggtagttgg cgatctgcac
    41281 ggatgctaca cgaacctgat gaaaaaactg gagacgatag gattcgacac caaaaaagac
    41341 ctgcttatct cggtgggcga tttggttgat cgcggtacag agaacgtaga atgcctggaa
    41401 ttaatcacat tcccctggtt cagagctgta cgtggaaacc atgagcaaat gatgattgat
    41461 ggcttatcag agcgtggaaa cgtcaatcac tggctgctta atggcggtgg ctggttcttt
    41521 aatctcgatt acgacaaaga aattctggct aaagctcttg cccataaagc agatgaactt
    41581 ccgttaatca tcgaactggt gagtaaagga aaaaaatatg tcatctgcca cgccgattat
    41641 ccttgtgata aatacgagtt tggaaagcca gttgatcatc agcaggtaat ctggaaccgc
    41701 gaacgaatca gcaactcaca agacgggatc gtgaaagaaa tcaaaggcgc ggacacgttc
    41761 atctttggtc atacgccagc agtgaaacca ctcaaatttg ccaaccagat gtatatcgat
    41821 actggcgcag tgttctgcgg aaacctcaca ttgattcagg tacagggaga aggcgcatga
    41881 gactcgaaag cgtagctaaa tttcattcgc caaaaagccc gatgatgagc gactcaccac
    41941 gggccacggc ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc
    42001 aatcacaagc cggattcgga atggctgtat tctgtggtaa gcacgaactc agccagaacg
    42061 acaaacaaaa ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc
    42121 gtggcgtggc aaagcttgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat
    42181 tcgcttatgc ggattattgc cgtagtgccg ctacgccggg cgcaagatgc agagattgcc
    42241 acggtacagg ccgtgcggtt gatatagcca aaacagagca gtgggggata gttgctgaga
    42301 aagagtgcgg aagatgtaaa ggcgtcggtt attcaagaat gccagcaagc gccgcatatc
    42361 gcgctgtgac gatgctaatc ccaaacctta cccaacccac ctggtcacgc actgttaagc
    42421 cgctgtatga cgctctggtt gtgcaatgcc acaaggaaga gtcaatcgca gacaacattt
    42481 tgaatgcgat cacacgttag cgccatgatt gccacggatg gcaacatatt aacggcataa
    42541 tattgacttt ttgaataact ttggggaaac ttgacaccaa taatgggcgt tttttacatg
    42601 tcattgatga gtctcaataa cctgccgccg agtagttttt atgctctgaa ttgtatttgt
    42661 gtagtaaaca tgctgactgc aatgtaatag agttttttta gcctgtaacc tcttgacggc
    42721 attgaattgc ttttgttatg agttgtaagc caatgttatc atcttgtatt ggggtggtta
    42781 tgaaggatgg tgcgctgctc aggagttctt cactttttat tgcctacatg ggatgccttg
    42841 gatgggggag tgcttatttc tatggatggg gtacttcttt ttactacggc ttcccatggt
    42901 ggattgtagg tgcaggtgtt gatgatgttg ccagaagttt attttttgca gttatcgtca
    42961 ttgctatatt tcttatcggt tggggtattg gtgttgtatt ctttttcgca gtgaaaagaa
    43021 aacattctat gcaagagcta aatgtatttc gcctttattt tgctgtggaa ttattgtttg
    43081 tgccggcaat tattgagttt tctatattga gacagaagat tcaggtacct cttttgctac
    43141 tgtcagcagc gattgcgctg gcggtcacaa tttcgataag atcttatggg cgatttttat
    43201 cggtatcatg cttctatgat aagccattta taaaaaaaca tttttttgag attgtgatga
    43261 ttgcttttgt ggcatatttc tggctttttt catttctgac aggatattac aaaccgcagt
    43321 ttaagaaaga atatgaaatg attaattata atgatggttg gtattatgtt cttgctcgtt
    43381 atgataattg tctggttttg tctacttctt tcaa
//
LOCUS       DAZFTN010000025        42712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFTN010000025 DAZFTN010000000
VERSION     DAZFTN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHKV2_001580"
     CDS             complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHKV2_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO6939485.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1955..2647
                     /locus_tag="ACHKV2_001581"
     CDS             1955..2647
                     /locus_tag="ACHKV2_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO6939486.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2846..3934
                     /gene="serC"
                     /locus_tag="ACHKV2_001582"
     CDS             2846..3934
                     /gene="serC"
                     /locus_tag="ACHKV2_001582"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO6939487.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4005..5288
                     /gene="aroA"
                     /locus_tag="ACHKV2_001583"
     CDS             4005..5288
                     /gene="aroA"
                     /locus_tag="ACHKV2_001583"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO6939488.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHKV2_001584"
     CDS             5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHKV2_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO6939489.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6394..7077
                     /gene="cmk"
                     /locus_tag="ACHKV2_001585"
     CDS             6394..7077
                     /gene="cmk"
                     /locus_tag="ACHKV2_001585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO6939490.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHKV2_001586"
     CDS             7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHKV2_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO6939491.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHKV2_001587"
     CDS             9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHKV2_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO6939492.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9512..11771
                     /locus_tag="ACHKV2_001588"
                     /pseudo
     CDS             9512..11771
                     /locus_tag="ACHKV2_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            11808..13556
                     /gene="msbA"
                     /locus_tag="ACHKV2_001589"
     CDS             11808..13556
                     /gene="msbA"
                     /locus_tag="ACHKV2_001589"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO6939493.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13553..14539
                     /gene="lpxK"
                     /locus_tag="ACHKV2_001590"
     CDS             13553..14539
                     /gene="lpxK"
                     /locus_tag="ACHKV2_001590"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO6939494.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14576..15808
                     /gene="ycaQ"
                     /locus_tag="ACHKV2_001591"
     CDS             14576..15808
                     /gene="ycaQ"
                     /locus_tag="ACHKV2_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO6939495.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15860..16042
                     /gene="ycaR"
                     /locus_tag="ACHKV2_001592"
     CDS             15860..16042
                     /gene="ycaR"
                     /locus_tag="ACHKV2_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO6939496.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16039..16785
                     /gene="kdsB"
                     /locus_tag="ACHKV2_001593"
     CDS             16039..16785
                     /gene="kdsB"
                     /locus_tag="ACHKV2_001593"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6939497.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16939..17832
                     /locus_tag="ACHKV2_001594"
     CDS             16939..17832
                     /locus_tag="ACHKV2_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO6939498.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17809..18588)
                     /gene="elyC"
                     /locus_tag="ACHKV2_001595"
     CDS             complement(17809..18588)
                     /gene="elyC"
                     /locus_tag="ACHKV2_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO6939499.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18724..19509
                     /gene="cmoM"
                     /locus_tag="ACHKV2_001596"
     CDS             18724..19509
                     /gene="cmoM"
                     /locus_tag="ACHKV2_001596"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO6939500.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19506..20828
                     /gene="mukF"
                     /locus_tag="ACHKV2_001597"
     CDS             19506..20828
                     /gene="mukF"
                     /locus_tag="ACHKV2_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO6939501.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20809..21513
                     /gene="mukE"
                     /locus_tag="ACHKV2_001598"
     CDS             20809..21513
                     /gene="mukE"
                     /locus_tag="ACHKV2_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO6939502.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21513..25973
                     /gene="mukB"
                     /locus_tag="ACHKV2_001599"
     CDS             21513..25973
                     /gene="mukB"
                     /locus_tag="ACHKV2_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO6939503.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26234..28081
                     /gene="ldtD"
                     /locus_tag="ACHKV2_001600"
     CDS             26234..28081
                     /gene="ldtD"
                     /locus_tag="ACHKV2_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6939504.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28262..28810
                     /locus_tag="ACHKV2_001601"
     CDS             28262..28810
                     /locus_tag="ACHKV2_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO6939505.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28837..29484
                     /gene="gloC"
                     /locus_tag="ACHKV2_001602"
     CDS             28837..29484
                     /gene="gloC"
                     /locus_tag="ACHKV2_001602"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO6939506.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29535..30725)
                     /gene="aspC"
                     /locus_tag="ACHKV2_001603"
     CDS             complement(29535..30725)
                     /gene="aspC"
                     /locus_tag="ACHKV2_001603"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO6939507.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30910..31998)
                     /gene="ompF"
                     /locus_tag="ACHKV2_001604"
     CDS             complement(30910..31998)
                     /gene="ompF"
                     /locus_tag="ACHKV2_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO6939508.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(32601..34001)
                     /gene="asnS"
                     /locus_tag="ACHKV2_001605"
     CDS             complement(32601..34001)
                     /gene="asnS"
                     /locus_tag="ACHKV2_001605"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO6939509.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(34170..35372)
                     /gene="pncB"
                     /locus_tag="ACHKV2_001606"
     CDS             complement(34170..35372)
                     /gene="pncB"
                     /locus_tag="ACHKV2_001606"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO6939510.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            35638..38250
                     /gene="pepN"
                     /locus_tag="ACHKV2_001607"
     CDS             35638..38250
                     /gene="pepN"
                     /locus_tag="ACHKV2_001607"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO6939511.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(38293..39060)
                     /gene="ssuB"
                     /locus_tag="ACHKV2_001608"
     CDS             complement(38293..39060)
                     /gene="ssuB"
                     /locus_tag="ACHKV2_001608"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6939512.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(39057..39848)
                     /gene="ssuC"
                     /locus_tag="ACHKV2_001609"
     CDS             complement(39057..39848)
                     /gene="ssuC"
                     /locus_tag="ACHKV2_001609"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO6939513.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(39860..41005)
                     /gene="ssuD"
                     /locus_tag="ACHKV2_001610"
     CDS             complement(39860..41005)
                     /gene="ssuD"
                     /locus_tag="ACHKV2_001610"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO6939514.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            complement(41002..41961)
                     /gene="ssuA"
                     /locus_tag="ACHKV2_001611"
     CDS             complement(41002..41961)
                     /gene="ssuA"
                     /locus_tag="ACHKV2_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO6939515.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(41954..42529)
                     /gene="ssuE"
                     /locus_tag="ACHKV2_001612"
     CDS             complement(41954..42529)
                     /gene="ssuE"
                     /locus_tag="ACHKV2_001612"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO6939516.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT        10487 a        10653 c        11316 g        10256 t
ORIGIN      
        1 aaaaaacgcc ccgtaatata actcagacga atcattaagc ctacattacg taggctattt
       61 gattttattt tgcccagaat gctgctttgg cgcgctgcag cttttcgtag gccttcaaca
      121 gcgactgatg tgcagcaaac gcgtgcagat cgctatctac tggttgcaag ccataaaacg
      181 ccgcttcgcc gctcattgcc gcactggcgg cttccacggc atctgcgccg tacatgcgaa
      241 caaacgcatt cagatattgc agcggctggc gatcttcttc ctgcgccagt aataacaacg
      301 tttgcaggca gcgataatag ttggcgcgtt ccgggctgaa taccgatgag ttaaactcca
      361 tcgtccattc ggtccagacc agagcctgtt ccagatcgcc accagccagc gccagcatgg
      421 cttttaattc accaatacgc agggtgtacc aaccgttatc cgacccggtc gccagaccca
      481 acagctcacg cacgcgggta aagtcatcaa aaccttcttc atccagttgc tcgatgaggt
      541 tcaggtaatc ttctttttcc cactcgctgc ctggtagcga aagaatcgtt tcacgtaaat
      601 ggctgcccat actgttattc gcgagccaca gatcttcagc cggataaata tcggacatgc
      661 caggcacgat aatacggcaa gcataaacgc ccagatgctc gtaatcggca atataaactt
      721 ctttatcttc tttgttgaag atagccatca gcgtagcaaa ctcttcttcc gtggtgccgg
      781 agaaattcca gtccacaaac ggataatcgg catcctgctt gaacaggtcc caggagatta
      841 aaccgctgga atcgataaag tgcgtttcga ggttggtatg ttcagcgact tcttcatcat
      901 cgaaggttgg cggagtaaac acatccaaat ctttcaggcc acgaccttgc agcagctcgg
      961 tcacggtacg ttccagtgct acgccaaaat caggatgcgc accgaaagag gcaaagcagg
     1021 tgccgttagc aggattaaac agtaccacgc aaatcaccgg atactggccg ccaagggaac
     1081 catcatatgc gaagatcggg aaaccttctg cttccagtgt ttcgatcgct tcaaccaccg
     1141 ctgggtaacg cgccagcacg tctgccggga tttccggcaa gctgatgctt tcagcaataa
     1201 tgcggttttt cacgtagcgt tcgaaaactt cggacaaccc ctgaacgcgt gcttcgttac
     1261 gggtattgcc agcagacata ccgttagaaa cgtacaggtt accaatgata ttcatcggaa
     1321 tataaacggt ctgattatca gactgacgtg taaacggcag accgcaaata ccacgatctt
     1381 cgttaccgga ttgtaggtca atcagcatac tgccggtcaa ttcattctcc ggatcgtaaa
     1441 atgcgcgcag acgttcatcg agcagccctt ctggcacatc gtcattttcg gtcagtggga
     1501 accatttttc gttgggataa tgcacaaatg gaccgttggc gatggtttcg cccagccaga
     1561 aatcagcaaa aaagtagttg gttgagagac gctcgaaata ttcaccgagt gcagaagcca
     1621 gcgccgcttt cttggttgcg cctttaccgt tggtaaaaca cagtgcgcac tctttgtcgc
     1681 gaatatgtac agaccagacg ttaggcacgg gattcagcca ggaggcctct tcaatctgaa
     1741 agccgaggtc tgaaagtttt tgctggaagc gagcgatgga atcttccagt gcggcatctt
     1801 tgccggggat aaatgtttgc gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata
     1861 atacgggttt tatctcaaag gcgctatcac cgccgccatg ccgatgaact gttgactatg
     1921 cttttagcgg ataaaccacg ttaagagcat aaaaatgaag gcgttcgatc tccaccgtat
     1981 ggcatttgat aaagtgcctt ttgatttcct tggcgaagtt gcactacgta gtctttatac
     2041 ctttgtactg gtctttttgt tcctcaaaat gaccggaaga cgcggtgtgc ggcagatgtc
     2101 gctgtttgaa gttttaatca ttctgacgct gggatcagcg gcgggagatg tggcgtttta
     2161 tgatgatgtg ccgatggtcc cggtacttat cgtctttatt actctggcgt tgttataccg
     2221 cctggtaatg tggttgatgg cgcacagtga aaaactggaa gatcttctgg aaggcaagcc
     2281 agttgtcatt attgaagatg gcgagctggc ctggtcgaaa ctcaataact ccaacatgac
     2341 ggaatttgag ttctttatgg agctacgatt gcgtggcgtg gagcagctgg ggcaggtacg
     2401 tctggcgatt ctcgaaacca acgggcaaat cagtgtctat ttctttgaag atgacaaggt
     2461 gaaatcgggt ttacttattt tacccagtga ttgtacgcag cgttacaaag tggtgccgga
     2521 gtcggcggac tatgcctgta ttcgttgtag tgaaatcatt catatgaaag cgggggaaaa
     2581 acaattatgt ccgcgctgtg caaatccaga atggacgaag gcaagtcggg caaaacgggt
     2641 gacctgacag taaaaacatc ggctttttgc taataatccg agagattctt ttgtgtgatg
     2701 caagccacat ttttgccctc aacggtttta ctcattgcga tgtgtgtcac tgaatgataa
     2761 aaccgatagc cacaggaata atgtattacc tgtggtcgca atcgattgac cgcgggttaa
     2821 tagcaacgca acgtggtgag gggaaatggc tcaaatcttc aattttagtt ctggtccggc
     2881 aatgctaccg gtagaggtgc ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct
     2941 tggtacgtcg gtgatggaag tgagtcaccg tggcaaagag ttcattcagg ttgcagagga
     3001 agccgagaag gattttcgcg atcttcttaa tgtcccctcc aactacaagg tattattctg
     3061 ccatggcggt ggtcgcggtc agtttgctgc ggtaccgctg aatattctcg gtgataaaac
     3121 caccgcagat tatgttgatg ccggttactg ggcggcaagt gccattaaag aagcgaaaaa
     3181 atactgcacg cctaatgtct ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa
     3241 gccaatgcgt gaatggcaac tctctgataa tgctgcttat atgcattact gcccgaatga
     3301 aaccatcgac ggtatcgcca tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc
     3361 cgccgacttc tcttcaacca ttctttcccg tccgattgac gtcagccgtt atggtgtaat
     3421 ttacgctggc gcgcagaaaa atatcggccc ggcaggcctg acaatcgtca tcgtccgtga
     3481 agatttgctg ggcaaagcga atgtcgcgtg tccgtcgatt ctggattatt ccatcctcaa
     3541 cgataacggc tccatgttta acacgccgcc gacatttgcc tggtatctat ctggtctggt
     3601 ctttaaatgg ctgaaagcga acggcggtgt agctgaaatg gataaaatca atcagcaaaa
     3661 agcagaactg ctatatgggg tgattgataa cagcgatttc taccgcaatg acgtggcgaa
     3721 agctaaccgt tcgcggatga acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt
     3781 gttccttgaa gagtcttttg ctgcaggcct tcatgcactg aaaggtcacc gtgtggtcgg
     3841 cggaatgcgc gcgtctattt ataatgccat gccgctggaa ggcgttaaag cgctgacaga
     3901 cttcatggtt gagttcgaac gccgtcacgg ttaatgccga aattttgctt aatccccaca
     3961 gccagcctgt ggggttttta tttctgttgt agagagttga gttcatggaa tccctgacgt
     4021 tacaacccat cgctcgtgtc gatggcacta ttaatctgcc cggttccaag agcgtttcta
     4081 accgcgcttt attgctggcg gcattagcac acggcaaaac agtattaacc aatctgctgg
     4141 atagcgatga cgtgcgccat atgctgaatg cattaacagc gttaggggta agctatacgc
     4201 tttcagccga tcgtacacgt tgcgaaatta tcggtaacgg cggtccatta cacgcagaag
     4261 gtgccctgga gttgttcctc ggtaacgccg gaacggcaat gcgtccgctg gcggcagctc
     4321 tttgtctggg tagcaatgat attgtgctga ccggtgagcc gcgtatgaaa gagcgcccga
     4381 ttggtcatct ggtggatgcg ctgcgcctgg gcggggcgaa gatcacttac ctggaacaag
     4441 aaaattatcc gccgttgcgt ttacagggcg gctttactgg cggcaacgtt gacgttgatg
     4501 gctccgtttc cagccaattc ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag
     4561 atacggtgat tcgtattaaa ggcgatctgg tttctaaacc ttatatcgac atcacactca
     4621 atctgatgaa gacgtttggt gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa
     4681 aaggcgggca gtcttatcag tctccgggta cttatttggt cgaaggcgat gcatcttcgg
     4741 cttcttactt tctggcagca gcagcaatca aaggcggcac tgtaaaagtg accggtattg
     4801 gacgtaacag tatgcagggt gatattcgct ttgctgatgt gctggaaaaa atgggcgcga
     4861 ccatttgctg gggcgatgat tatatttcct gcacgcgtgg tgaactgaac gctattgata
     4921 tggatatgaa ccatattcct gatgcggcga tgaccattgc tacggcggcg ttatttgcaa
     4981 aaggcaccac cacgctgcgc aatatctata actggcgtgt taaagaaacc gatcgcctgt
     5041 ttgcgatggc aacagaactg cgtaaagtcg gtgcggaagt agaagagggg cacgattaca
     5101 ttcgtatcac tccaccggaa aaactgaatt ttgccgagat cgcgacatac aatgatcacc
     5161 ggatggcgat gtgtttctcg ctggtggcgt tgtcagatac accagtgacg attcttgatc
     5221 ccaaatgtac ggccaaaaca tttccggatt atttcgagca gctggcgcgg attagccagg
     5281 cagcctgaat gaataacgtc caataaatag ccaaaacttt ctttatcaaa acgtcggcac
     5341 attgccggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
     5401 caatgtaagt tgttgataca taatgtttat atatgattaa tcaacggatg attcacatga
     5461 agaatactaa attactgctg gcgattgtga cctctgcagc attactgacg gggtgtcaaa
     5521 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
     5581 caacgttaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
     5641 gcggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
     5701 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
     5761 acgtcaacgc atgggcgatg gcgaacggct gtgttcgtgt ttacagtggc ctgatggaca
     5821 tgatgaacga taacgaaatt gaaggcgttc tgggccatga actgggccac gtcgcgttgg
     5881 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
     5941 cagctaccag cggtgtggct tcccagcttt cccgctcaca attgggtgat atcgcagaag
     6001 gcgctatcaa tgcgaaatac tcccgcgata aagagtccga agcagatgat ttctcctttg
     6061 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
     6121 ctagcctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
     6181 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
     6241 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
     6301 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     6361 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     6421 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg
     6481 caatggcatc tgctggattc gggtgcaatt tatcgcgtac tggcactagc ggcattacat
     6541 caccatgttg atgttgcatc ggaagatgcg ctggtgccgc tggcatccca tctggatgta
     6601 cgttttgtgt caaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     6661 gaaattcgta ctcaggaagt ggcgaatgca gcttcacaag tcgcggcatt cccacgcgtt
     6721 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat
     6781 ggccgcgaca tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc
     6841 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     6901 aactttgagc gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     6961 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     7021 gagcaagtga ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg
     7081 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     7141 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     7201 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     7261 gtggcgttgt tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg
     7321 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     7381 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     7441 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     7501 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     7561 acggtattcg tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc
     7621 tgcacctgga aggcaaagag cttgaattca aagtaatcaa gctggatcag aagcgcaaca
     7681 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     7741 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     7801 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct
     7861 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     7921 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag
     7981 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     8041 ccaacctgac cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac
     8101 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     8161 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg
     8221 gtctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg
     8281 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     8341 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     8401 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     8461 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     8521 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     8581 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     8641 gtgaccgcgt tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     8701 tcaccggcgt tgatcgtaaa aaccgtgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     8761 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     8821 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     8881 ttttattctg aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     8941 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     9001 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     9061 aatcgcacat tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     9121 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     9181 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag
     9241 gaaaatacgt tcctcacttt aaacctggta aagaactgcg cgatcgcgcc aatatttacg
     9301 gttaagtttt tactcaaact tgaacgagag aaaagcacct gtcgggtgct tttttcattt
     9361 atctaatctg gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     9421 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     9481 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     9541 aatttgcgga atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc
     9601 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     9661 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgttggc
     9721 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg ctactgacgg
     9781 catgaccact cattacggtc aaattactta tctacaaggt caacgtatat tccctgcgcc
     9841 aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgccggac aactgtggtc
     9901 aatgaaactc aaagttcgtg ccgttcatgg tcaacttaat gatggcggct ttgatagcca
     9961 gcgttatgcc attgcccagc atcagccgct caccggccgc tttctgcagg caagtgttat
    10021 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
    10081 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccctaa
    10141 agaaatcaaa aatatcatgc gtgatactgg aacggcgcat ttaatggcga tatcgggatt
    10201 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
    10261 gcctgggcgc tggatccact ggcaaatgcc attaattggc ggaatctgct gtgctgcttt
    10321 ttatgcctgg ctgacgggaa tgcaacctcc tgcattgcgt accgttgtgg cgcttgctac
    10381 gtggggtatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
    10441 tctggcggca attttgctga tggatcctgt tgtcattctc tcgcaaagtt tatggctctc
    10501 tgccgctgcg gtcgcggcat tgatattttg gtatcagtgg tttccctgtc ctgagtggca
    10561 actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg gaatcacact
    10621 tctgcttatg cccgtgcaaa tcgtcatttt tcatggcatt agtctgacct cgtttattgc
    10681 aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat
    10741 ggttgtgcat ttaagcgggc cgttaatcct ggatcaaggg ttatggtttc ttgccgaccg
    10801 gtctttggct ttacttttct tggggttaaa gagtttgcca gaagggtgga tcaacattgc
    10861 tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa
    10921 cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct
    10981 gtggcaaaaa cctcgacctg acgagtggca agtgtacatg cttgatgtcg ggcaagggct
    11041 ggcaatggtg atagccagaa acggcaaagc gattctctat gacacgggac tggcctgaag
    11101 gggatagtgg gcaacaactg attatcccct ggctccactg gcataatctt gaaccggaag
    11161 gcgttatcct gagtcatgaa catctggatc accggggagg gctggactca atattgcata
    11221 catggccgat gttatggatc agaagtccgt taaactggga acatcatcag ccctgtgtgc
    11281 gtggcgaagc gtggcaatgg caaggattgc gtttcagcgc gcactggcct ttacaaggtc
    11341 gcaacgataa aggaaataac cattcctgtg tggttaaggt tgatgacggg acgaatagca
    11401 ttcttctaac cggtgatatt gaagccccag ctgaacaaaa gatgctaagc cgttactggc
    11461 agcaagtgca ggcaacattg cttcaggtac ctcaccatgg cagtaatacc tcatcatcat
    11521 tgccattaat tcagcgagtg aatggaaaag tggcactcgc atcggcatcg cgctataacg
    11581 catggcgact gccctctaac aaagttaagc atcgctatca acaacaaggt tatacgtggc
    11641 ttgatactcc tcatcagggg caagtaacgg tcgatttttc agcgcaaggc tggcggatta
    11701 gcagcctcag ggagcaaatt ttacctcgtt ggtatcatca gtggtttggc gtgccagtgg
    11761 ataacgggta gaatatgcgg ctatttcaac aaatgctggt tttttgaatg cataacgaca
    11821 aagatctctc tacgtggcag acattccgcc gactgtggcc aaccattgcg ccttttaaag
    11881 cgggtctgat cgtggcgggc gtagcgttaa tcctcaacgc agccagcgat accttcatgt
    11941 tatcgctcct taagccactt cttgatgatg gctttggtaa aacagatcgc tccgtgctgg
    12001 tgtggatgcc gctggtggtg atcggactga tgattttgcg tggtatcacc agctatgtct
    12061 ccagctactg tatctcctgg gtatcaggaa aggtggtaat gaccatgcgt cgccgcctgt
    12121 ttggtcacat gatgggaatg ccggtttcat tctttgacaa acagtcaacg ggtacgctgt
    12181 tgtcacgtat cacctacgat tccgaacagg ttgcttcttc ctcttccggc gcactgatta
    12241 ctgttgtgcg tgaaggtgcg tcgatcatcg gcctgttcat catgatgttc tattacagtt
    12301 ggcaattgtc gatcattttg attgtgctgg caccgattgt ttcgattgcg attcgcgtag
    12361 tatcgaagcg ttttcgcaac atcagtaaaa acatgcagaa caccatgggg caggtgacca
    12421 ccagcgctga acaaatgctg aaaggccata aagaagtatt gattttcggt ggtcaggaag
    12481 tggaaacgaa acgcttcgat aaagtcagca accgaatgcg tcttcagggg atgaaaatgg
    12541 tttcagcctc ttccatctct gatccgatca ttcagctgat cgcctctttg gcgctggcgt
    12601 ttgttctgta tgcggcgagc ttcccaagtg tcatggatag cctgactgcc ggtacgatta
    12661 ccgttgtttt ctcttcaatg attgcactga tgcgtccgct gaaatcgctg actaacgtta
    12721 acgcccagtt ccagcgcggt atggcggctt gtcagacact gtttaccatt ctggacagtg
    12781 agcaggagaa agacgaaggt aagcgcgtga tcgagcgtgc gactggcgac gtggaattcc
    12841 gcaacgtcac ctttacttat ccgggacgtg acgtacccgc attgcgtaac atcaacctga
    12901 aaattccggc agggaaaacg gtcgctctgg ttggacgctc tggttcaggt aaatcaacca
    12961 tcgccagcct gatcacgcgt ttttacgata ttgatgaagg cgaaatcctg atggatggtc
    13021 acgatctgcg cgagtatacc ctggcgtcgt tgcgtaacca ggttgctctg gtgtcgcaga
    13081 atgtccatct gtttaacgat acggttgcta acaacattgc ttacgcacga actgaacagt
    13141 acagccgtga gcaaattgaa gaagcggcgc gtatggccta cgccatggac ttcatcaata
    13201 agatggataa cggtctcgat acagtgattg gtgaaaacgg cgtactgctc tctggcggtc
    13261 agcgtcagcg tattgctatc gctcgagcct tgttgcgtga tagcccgatt ctgattctgg
    13321 atgaagctac ctcggctctg gataccgaat ccgaacgtgc gattcaggcg gcactggatg
    13381 agttgcagaa aaaccgtacc tctctggtga ttgcccaccg cttgtctacc attgaaaagg
    13441 cagacgaaat cgtggtcgtc gaggatggtg tcattgtgga acgcggtacg cataacgatt
    13501 tgcttgagca ccgcggcgtt tacgcgcaac ttcacaaaat gcagtttggc caatgatcga
    13561 aaaaatctgg tctggtgaat cccctttgtg gcggctattg ctgccactct cctggttgta
    13621 tggcctggtg agtggcgcga tccgtctttg ctataaacta aaactgaagc gcgcctggcg
    13681 tgcccccgta ccggttgtcg tggttggtaa tctcaccgca ggcggcaacg gaaaaacccc
    13741 ggtcgttgtc tggctggtgg aacagttgca acagcgcggt attcgcgtgg gggtcgtatc
    13801 gcggggatat ggtggtaagg ctgaatctta tccgctgtta ttgtcggcag ataccaccac
    13861 agcacaggcg ggtgatgaac ctgtgttgat ttatcaacgc actgatgcgc ctgttgcggt
    13921 ttctcccgtt cgttctgatg cggtaaaagc cattctggcg caacaccctg atgtgcagat
    13981 catcgtaacc gacgacggtt tacagcatta ccgtctggcg cgtgatgtgg aaattgtcgt
    14041 tattgatggt gtgcgtcgct ttggcaatgg ctggtggttg ccggcggggc caatgcgtga
    14101 gcgagcgggg cgcttaaagt cagttgatgc ggtaatcgtc aacggcggtg tcccccgcag
    14161 cggtgaaatc cccatgcatc tgctgccggg tcaggcggtg aatttacgta ccggtacgcg
    14221 ttgtgacgtt gctcagcttg aacatgtggt ggcgatggca gggattgggc atccgccgcg
    14281 cttttttgcc acgctgaaga tgtgcggcgt acaaccggaa aaatgtgtac cgctggccga
    14341 tcatcagtct ttgaaccatg cggatgtcag cgcgttggta agcaccgggc aaacgctggt
    14401 aatgactgaa aaagatgcgg tgaaatgccg ggcctttgca gaagaaaatt ggtggtattt
    14461 gcccgttgac gcacagcttt caggtgatga accagcgaaa ctgcttgcgc aactaacctc
    14521 gctggcttct ggcaactagt tacgccgcgg cagcgttcga ttgatggagt catgaatgtc
    14581 gctgccgcac ctctcccttg ctgatgcgcg taatcttcac cttgccgcgc aaggcctgtt
    14641 aaacaaaccc cgccgtcgag cgtcgttgga ggatattccg gcaacgatct cccgcatgtc
    14701 cttgctgcaa atcgatacca tcaatattgt tgcccgtagc ccatatctgg tgcttttcag
    14761 tcgtctggga aattatcctg cccagtggct ggatgagtct ctggcgcgtg gcgaattaat
    14821 ggaatactgg gcgcatgaag cctgctttat gccacgcagt gactttcgtc ttattcgcca
    14881 ccgcatgctg gcacctgaaa aaatgggctg gaaatacaaa gacgcctgga tgcaggaaca
    14941 tgaggcggaa attgcacagt taattcagca tattcatgat aaggggccgg tacgttcagc
    15001 cgattttgag tatcctcgta aaggtgcaag cggctggtgg gaatggaagc cgcataaacg
    15061 gcatctggaa ggtttattta ctgccggaaa ggtgatggtg attgaacggc gcaacttcca
    15121 gcgcgtttat gatttaaccc accgtgtcat gcctgactgg gatgatgagc gcgatctcgt
    15181 ttcgcaaaca gaagcagaaa tcatcatgct ggataacagt gcgcgtagcc agggaatatt
    15241 ccgcgaacag tggctggcag attactatcg gctgaaacgt ccggcactgg cagcgtggcg
    15301 cgaagcgagg gctgaacagc ggcaaatcat tgctgtgcat gttgaaaaat tgggcaatct
    15361 ttggctgcat gctgatttgc tgccgctact cgagcgagcg ctggccggaa agctcactgc
    15421 aacgcacagc gcggtacttt cgccttttga tcctgttgtc tgggatcgca aacgcgcaga
    15481 gcagcttttt gattttagct accggctgga gtgctatacc ccagcgccga aacgccagta
    15541 tggctatttt gttctgccgt tattacatcg tgggcaatta gttgggcgaa tggatgccaa
    15601 aatgcatcgc cagacaggca tccttgaagt tatctctctg tggttacagg aaggtattaa
    15661 accaacgaca acgctgcaaa aagggttacg tcaggcgatt actgatttcg ctaactggca
    15721 gcaggcaacg cgggtgacat taggacactg cccgcaaggt ctctttacgg attgccgcac
    15781 cggctgggaa atagaccccg tcgcataaat gattatgata agatttatgc attcataacc
    15841 cgatccatct ggaggaacta tggatcatcg tctgcttgaa atcattgcct gcccggtttg
    15901 caacggaaaa ctttggtata accaggaaaa acaagaactt atttgcaaac tggacaacct
    15961 ggctttcccg ctgcgcgatg gcattccagt gttgctggaa acggaagccc gcgtgctgac
    16021 tgctgatgag agtaaatcat gagttttgtg gtcattattc ccgcgcgcta cgcgtccacg
    16081 cgtctgcccg gtaaaccatt ggttgatatt aacggcaaac ccatgattgt tcatgttctt
    16141 gaacgcgcgc gcgaatcagg tgccgagcgc atcatcgtgg caaccgatca tgaggatgtc
    16201 gcccgcgccg ttgaagccgc tggcggtgaa gtatgtatga cgcgcgccga tcaccagtca
    16261 gggacagaac gcttggcgga agttgtcgaa aaatgcgcgt tcagcgacga cacggtgatc
    16321 gttaatgtgc agggtgatga accgatgatc cctgcgacaa tcattcgtca ggttgctgat
    16381 aacctcgctc agcgtcaggt gggtatggcg actctggcgg tgccaatcca caatgcggaa
    16441 gaagcgttta acccgaatgc ggtgaaagtg gttctcgacg ctgaagggta tgcactgtac
    16501 ttctcacgcg ctaccattcc gtgggatcgt gatcgttttg caaaaggcct tgaaaccgtt
    16561 ggcgataact tcctgcgtca tcttggtatt tatggctacc gtgcaggctt tatccgtcgt
    16621 tacgtcacct ggcagccaag cccgttagaa cacatcgaaa tgttagagca gcttcgtgtt
    16681 ttgtggtacg gcgaaaaaat ccatgttgct gtcgcgcatg aagttcctgg cacaggtgtg
    16741 gatacccctg aagatctcga gcgcgttcgc gctgaaatgc gctaatttca cttcacgaca
    16801 cttcagccaa ttttgggagg agtgtcgtac cgttacgatt ttcctcaatt tttcttttca
    16861 acaattgatc tcattcaggt gaaatctttt atattggcgc tcattatgaa agcagtagct
    16921 tttatgaggg taatctgaat ggaacagctg cgtgccgaat taagccattt actgggcgaa
    16981 aaactcagtc gtattgagtg cgtcaatgaa aaagcggata cggcgttgtg ggctttgtat
    17041 gacagccagg gaaacccaat gccgctaatg gcaagaagct ttagtacgcc cggaaaagcc
    17101 cgacaactgg catggaaaac caccatgctg gcaagaagtg ggactgtccg tatgccgact
    17161 atttatggtg tgatgacgca tgaagaacac cccggccctg atgtcctgct actggagcgg
    17221 atgcgtggtg tttcggtaga ggcaccagcc cgaacaccag aacgttggga acagctcaaa
    17281 gaccaaatcg ttgaagcctt actggcctgg caccgtcagg acagtcgcgg ttgcgtcggc
    17341 gcggtcgaca atactcagga aaatttctgg ccctcatggt accggcaaca tgttgaagtg
    17401 ctatggacca cgctcaatca gttcaataac accggtctga cgatgcagga taagcggatc
    17461 ctgtttcgca ctcgcgaatg tctcccggca ttatttgaag gttttaacga caattgtgtg
    17521 ctgattcacg gtaacttctg tttacgcagc atgttgaaag attcgcgcag cgatcagtta
    17581 ctggcgatgg tcgggccggg actaatgctt tgggcaccgc gagaatacga actgttccga
    17641 ctaatggata attctctggc ggaagatttg ctctggagtt acctgcaacg cgcgccagtg
    17701 gcggagtcgt tcatctggcg gcgttggttg tatgtgttat gggatgaagt tgcgcaactg
    17761 gttaataccg gacgatttag tcggcgcaac ttcgatctgg catcaaaatc actcttgccg
    17821 tggctcgcct gacgaacctt ttagccactg ccagatgcgg ccaagcgttt catagccaac
    17881 acgatcgcta tgcatcaacc agaccggaga agggattgcc cgttcccacg ggttaagcgg
    17941 cgagtcgatg gccaactgat tcgccggggc tggtagtgga tttaaccctt cctgctgaaa
    18001 aaagatcatc gcccgcggca gatgtgatgc ggaggtcacc agcaggaaag gggcgtcacc
    18061 aatcgcctgt ttcactgccg cagcttcttc ttcggtatct tttggcaaat ccagggtgat
    18121 aatttgctcg cgcggtacgc ccagcgattg cgcaactctg gcacctactt ccgctgtact
    18181 caccgtattg gtttttgcta cgcctcccgt gaagatcagt tttgaacccg gattttcgcg
    18241 ccataaacgg ataccctcat tcaggcgtgg cagactgtta ttgattaaat tagagctcgg
    18301 tgcccactgc gggttccagg tataaccacc tccgagcacc acgatatagt ccactttttg
    18361 cgaattattc catgtcggat aggtgctttc gatgggacgc agcaggcggt cggctaccgg
    18421 ttgcaaactc aataacaaga gtgccagcca cccaatgcta ataaaaatct tcccggtttt
    18481 ttgaaaacgg ctaaaccaca gtagcgccag gccagcgcca ataattaaca acatcagcgg
    18541 aagtggcagc aacatattgc caatcacttt tttcagtgta aaaagcatcc tttctggttc
    18601 cttttttaac catatagcaa gggatcgcca gcgatattac accagacggg ttcattctcg
    18661 ccgtggctgt gacaaaatag cgtctttatc agaaaacgtc ttagccaacc ggtggagaaa
    18721 gcaatgcagg atcgcaattt tgatgatatt gcggaaaagt tttcccgtaa catttacggc
    18781 accaccaaag ggcagcttcg acaggctatt ctgtggcagg atctcgatcg cgtgctggcg
    18841 gaaatgggtc cgcaaaaact gcgtgtgctg gatgcgggcg gtggagaagg gcagaccgca
    18901 atcaaaatgg ccgagcgcgg gcatcaggtc attttatgcg atctttctgc gcagatgatc
    18961 gaccgcgcaa aacaggcggc agaagcaaaa ggtgtgagcg acaacatgca atttatacat
    19021 tgcgccgctc aggatgttgc ttcgcatttg gaaacgcccg ttgatctgat attgttccat
    19081 gcggtgctcg agtgggtggc tgatccccgc agcgtattgc agaccctgtg gtcagtgttg
    19141 cgtccaggcg gcgtgttgtc gttaatgttc tacaatgcgc atggtttgtt gatgcataac
    19201 atggtcgccg ggaattttga ttacgtgcag gcgggaatgc cgaaaaagaa aaaacggacg
    19261 ctttcgccag attatccacg cgacccgacg caggtttatc tgtggctgga agaagctggt
    19321 tggcaaatta tgggtaagac aggcgttcgc gtgtttcatg attatctgcg cgagaagcac
    19381 cagcagcgcg actgctatga agcattactt gaattagaaa cgcgttattg ccgtcaggaa
    19441 ccgtatatta ccctggggcg ttatattcat gtcaccgcgc gcaaaccgca gagcaaggat
    19501 aaagtatgag tgaattttcc cagacagtcc ccgaactggt tgcctgggcc agaaaaaatg
    19561 acttctccat ttcgctgccg gtagaccgac tctcttttct gctggcagtt gccacgctga
    19621 acggcgagcg tctggatggt gagatgagtg aaggcgagct ggtggatgca ttccgccatg
    19681 tgagtgatgc gtttgagcaa accagcgaaa ccatcggcgt gcgcgccaac aacgcgatca
    19741 acgacatggt gcgtcaacgt ctgctgaacc gctttaccag cgagcaggca gaagggaacg
    19801 caatttaccg tctgacgccg ctcggcatcg gcattactga ctattacatc cgtcagcgcg
    19861 agttttctac gctgcgcctt tctatgcagt tgtcgattgt ggcgggtgag ctcaaacgcg
    19921 cagcagatgc cgccgaagag ggcggtgatg aatttcactg gcaccgtaat gtctatgcgc
    19981 cactgaaata ttcggtagca gaaattttcg acagtatcga cctgacgcaa cgtctgatgg
    20041 acgaacagca gcagcaggtg aaggacgata tcgcccagtt gctgaacaaa gactggcggg
    20101 cggcgatttc cagctgtgaa ttgttgcttt cggaaacttc cggaacgctg cgtgaattgc
    20161 aggatacact ggaagcggca ggcgacaaat tgcaggctaa tctgttgcgc attcaggatg
    20221 cgacgatgac tcatgacgat ctgcatttcg tcgatcgtct ggtgttcgat ctgcagagca
    20281 aactcgaccg tattatcagt tggggccagc aatccatcga tttgtggatt ggctacgacc
    20341 gccacgtaca taaatttatt cgtaccgcga tcgatatgga taaaaaccgc gtctttgctc
    20401 agcggttacg tcagtcggta caaacctatt ttgatgagcc gtgggcgcta agttatgcca
    20461 atgccgatcg tctgctggat atgcgtgacg aagagatggc actgcgcgat gaagaagtga
    20521 ctggggaact tccgccagat ttggaatacg aagagtttaa cgagatccgc gaacagctgg
    20581 cggcgatcat cgaagaacaa cttgccgtgt acaaaaccag acaagtgccg ctggatcttg
    20641 gtctggtggt acgcgaatat ctgtcacagt atccgcgtgc acgtcacttt gacgttgcgc
    20701 gcattgttat tgatcaggcg gtacgtcttg gcgtagcgca agcagatttc accggactgc
    20761 cagcgaaatg gcagccgatt aatgattacg gagccaaggt acaggcgcat gtcatcgaca
    20821 aatattgaac aagtgatgcc ggttaagctg gcgcaggcgc tggcgaatcc gttatttccg
    20881 gcgctggaca gcgccttacg ttcaggccgc catattggcc tcgacgaact ggataatcat
    20941 gcattcctga tggattttca ggaatatctg gaagagtttt acgcgcgtta taacgttgag
    21001 cttattcgtg caccggaagg gttcttctat ttacgtccac gttccaccac gctgatccct
    21061 cgttccgtct tgtcggaact ggatatgatg gtcgggaaaa tcctctgtta tctctatctc
    21121 agcccggaac ggctggcgaa tgaggggatt ttcacccagc aggaactgta cgacgaactg
    21181 ctcaccctgg ccgatgaagc gaaactgctg aaactggtga acaaccgttc aaccggttca
    21241 gacattgacc gtcagaagtt gcaggaaaaa gtacgttctt cgctcaaccg tctgcgtcgt
    21301 ttaggtatgg tgtggtttat gggccacgac agcagcaagt tccgcattac cgaatcggtg
    21361 ttccgcttcg gagccgatgt gcgtgctggc gacgatcccc gtgaagcaca gcgtcgcctg
    21421 attcgtgatg gcgaagcaat gccgattgaa aatcatctgc aactcaacga tgaaaccgaa
    21481 gagaatcagc cagatagcgg agaggaagaa taatgattga acgcggtaaa tttcgctcac
    21541 tgacgctgat taactggaac ggcttctttg cccgaacttt tgaccttgac gagctggtca
    21601 cgacgctttc tggcggtaac ggggcgggta aatccaccac catggcggcg ttcgttacgg
    21661 cgctgatccc cgacctgacc ctgctgcatt tccgtaacac tacggaagcc ggggccacca
    21721 gcggttcgcg cgataaaggt ctgcacggta agctgaaagc gggtgtctgt tattcgatgc
    21781 tcgacaccat taactcgcgc caccagcgcg tggtggtcgg tgtgcgtctg caacaggttg
    21841 ccggacgcga tcgtaaagtg gatatcaaac catttgccat tcagggactg ccgatgtcgg
    21901 tgcagccgac acagctggtg accgaaactc tgaacgaacg ccaggcgcgc gtgctgccgc
    21961 ttaacgagct gaaagacaag ctcgaggcga tggaaggcgt gcagtttaaa cagttcaact
    22021 ccattaccga ttaccactcg ctgatgttcg atctgggcat cattgcgcgt cgtctgcgct
    22081 ccgcatctga ccgtagcaaa ttctatcgtc tgatcgaagc ttcgctgtat ggcgggatct
    22141 ccagtgccat tacccgttct ctgcgcgact acctgttgcc ggaaaacagc ggcgtgcgta
    22201 aagcgttcca ggacatggaa gcggcgctgc gtgaaaaccg catgacgctg gaagcgattc
    22261 gtgtcaccca gtcggaccgt gacctgttta agcatctgat cagcgaagct accaactacg
    22321 tggcggcgga ctacatgcgt cacgccaacg agcgccgtgt ccatctcgat aaggccctgg
    22381 agtttcgtcg cgaactacat acttcgcgtc agcaactggc ggcggagcag tacaaacacg
    22441 tcgatatggc gcgtgagctg gcagagcaca acggtgccga aggcgatctg gaagcggatt
    22501 atcaggcggc cagcgatcac ctgaacctgg tgcaaaccgc actgcgtcag caggagaaga
    22561 tcgaacgcta cgaagcggat ctcgatgagc tgcagatccg tctggaagag caaaacgaag
    22621 tggtggcaga agccatcgaa cgccaggaag agaacgaggc tcgcgcggaa gctgccgaac
    22681 tggaagtgga cgagctgaaa agccagcttg ctgactacca gcaggcgctg gacgtccagc
    22741 aaacgcgcgc gatccagtac aaccaggcga ttgctgcgct taatcgtgcc aaagaactgt
    22801 gccatctgcc ggacttaacc gccgacagcg ccgccgaatg gctggaaacc ttccaggcga
    22861 aagagctgga agcgactgaa aaaatgctct ctcttgagca gaaaatgagc atggcgcaaa
    22921 ccgcgcacag ccagtttgag caggcttatc agctggtggt ggcaatcaac ggcccgctgg
    22981 cgcgtaacga ggcgtgggat gtcgctcgcg aactattgcg cgaaggggtc gatcagcgtc
    23041 acctggcaga gcaggttcag ccgttgcgga tgcgcttaag cgaactggaa cagcgtctgc
    23101 gcgaacagca agaagctgag cgtctgctgg cagatttctg caaacgtcag ggcaagaatt
    23161 ttgacattga cgagctggaa gccctgcatc aggagctgga agcacgcatt gcctctcttt
    23221 ccgatagcgt gtctaacgcc cgtgaagagc ggatggcact gcgccaggag caggaacagc
    23281 tgcagtctcg cattcagagt ttgatgcagc gtgcaccggt ttggctggca gcgcaaaaca
    23341 gtctcaacca gttgagcgaa cagtgcggcg aagagttcac ctccagccag gacgtcactg
    23401 aatatctgca acagttgctg gagcgtgagc gcgaggccat tgttgaacgc gatgaagtag
    23461 gcgcgcgcaa aaacgccgtc gatgaagaga tcgaacgttt aagccagcct ggcggctctg
    23521 aagatcagcg tctgaacgcg ctagcggagc gttttggtgg tgtgctgctg tcagaaattt
    23581 atgacgacgt tagcctggaa gatgcgccgt acttctcagc gctgtatggc ccgtcacgcc
    23641 acgccatcgt ggtgccagat ctgtcacagg taaccgaaca cctggaaggt ttgaccgatt
    23701 gcccggaaga tctctatttg atcgaagggg atccgcagtc attcgatgac agcgtgttca
    23761 gcgttgatga gctggaaaaa gcggtagtgg tgaaaatcgc cgatcgtcag tggcgttatt
    23821 cacgtttccc ggaagtgccg ctgtttggtc gtgctgcgcg tgaaagccgt attgaaagcc
    23881 tccatgccga gcgtgaagtg ctttccgaac gcttcgccac gctctccttt gatgtacaga
    23941 aaacccagcg tctgcatcag gcgttcagcc gctttatcgg cagtcatctg gcggtggcgt
    24001 ttgagtctga cccggaagca gaaatccgtc aactgaacag ccgtcgcgtc gaactggagc
    24061 gggcgttgag taatcatgaa aatgataacc agcagcagcg tattcagttt gagcaggcga
    24121 aagaaggcgt tacggcgctg aaccgcattc tgccgcgtct caacctgttg gctgatgaca
    24181 gcctggcgga tcgcgtcgat gaaatccgcg aacgtctgga tgaagcccag gaagccgcgc
    24241 gttttgttca gcagtttggc aatcaactgg cgaaactgga accgatcgtt tcggtattgc
    24301 agagcgaccc ggaacagttc gaacagttaa aagaagatta cgcgtactct cagcagatgc
    24361 agcgcgatgc ccgtcagcag gcgtttgccc tgacggaagt ggtgcagcgt cgtgcgcact
    24421 ttagctactc tgactcggca gaaatgctta gcggtaacag cgatctcaac gaaaaactgc
    24481 gtgaacgtct ggaacaggcg gaagcggagc gtacccgcgc tcgcgaagcg ttgcgcggtc
    24541 acgcagcgca gttgagtcag tacaaccagg tgctggcttc gctgaaaagt tcttacgaca
    24601 ccaaaaaaga gctactcaac gatctgcaac gtgaattgca ggatatcggc gtgcgtgctg
    24661 atagcggggc agaagagcgg gcacgtattc gccgtgacga gctgcatgca caactgagca
    24721 ataaccgttc gcgccgcaat caactggaaa aagcgctcac cttctgcgaa gcggagatgg
    24781 ataacctgac ccgcaaactg cgcaagctgg agcgtgatta ctttgagatg cgcgagcagg
    24841 tagtgaccgc gaaagcgggc tggtgtgcag tcatgcgcat ggtgaaagat aacggcgttg
    24901 agcgccgctt acaccgtcgt gagctggctt atctctccgc tgatgatttg cgttccatgt
    24961 cggataaggc gttaggtgcg ctgcgtctgg cggtggcgga taacgaacat ctgcgcgacg
    25021 tgctgcgcat gtcggaagat cccaaacgtc cggagcgtaa aattcagttc ttcgtggcgg
    25081 tttatcagca tctgcgtgaa cgtattcgtc aggatattat tcgtaccgat gatccggtgg
    25141 aagctatcga acagatggag atcgaactta gccgtctgac cgaagagtta acctcccgtg
    25201 aacagaaact ggcgatcagt tcccgcagcg tggcgaacat cattcgcaaa accattcagc
    25261 gcgagcagaa ccgtatccgt atgctcaacc aggggttgca gaacgtatcg tttggtcagg
    25321 tgaacagcgt gcgcctcaac gtgaacgtgc gtgaaacgca cgccatgctg ctggatgtgc
    25381 tctctgaaca gcatgagcag catcaggatc tgtttaacag caaccgtctg accttctcgg
    25441 aagcgctggc gaaattgtat cagcgtctta atccgcagat tgatatgggg cagcgcacgc
    25501 cgcagaccat tggcgaagaa ctgctggatt accgcaacta tctggaaatg gaagttgagg
    25561 ttaaccgtgg ttccgatggc tggttgcgcg cagagtctgg tgcattgtcg accggtgagg
    25621 cgattggtac cggtatgtcg attctggtga tggtggtaca aagctgggaa gatgaatctc
    25681 gccgcctgcg cggtaaagat atctctcctt gccgcctgct gttcctcgat gaagcagcgc
    25741 gactggatgc tcgttctatc gccacgctgt ttgaattgtg tgagcgtttg caaatgcagc
    25801 tcatcatcgc agcgccggaa aatatcagcc cggagaaagg caccacctat aaactggtgc
    25861 gtaaagtctt ccagaatacc gaacatgttc atgtcgtcgg cctgcgagga tttgcgccgc
    25921 aactccctga aacgcttcca ggaagtgacg aagcgccttc tcaggcgagt taaaattaag
    25981 gcggcagcaa tgccgccttt tctttttccg aaaactccgt ttctgcacta aaaaagtggc
    26041 acattacggc gcgcttttct ttaaacttct ttacattagg ttatgtaaaa acgtatcggc
    26101 gtttatatac tgaagataag cctgatgagt aacaggcttg ctcgtcatac tttcgtgagt
    26161 attggcgttg tacaggcaag tcgtaaaata acagcctggc tattcagagt atgataaaaa
    26221 cagggggcaa gggatgttgc ttaatatgat gtgtggtcgt cggctgtcgg caatcagttt
    26281 gtgcctggcc gtaacattcg ctccactgtt caatgcgcag gccgatgagc ctgaagtaat
    26341 ccctggcgac agcccggtgg ctgtcagtga acagggcgag gcactgccgc aggcgcaagc
    26401 cacggcaata atggcgggga tcctgccatt gcctgaaggt gcggcagaaa aagcccgcac
    26461 gcaaatcgaa tctcaattac ccgcaggtta caagccggtt tatcttaacc agcttcaact
    26521 gttgtatgcc gcacgcgata tgcaacccat gtgggaaaac cgtgatgctg ttaaagcctt
    26581 ccagcaacag ctggcagagg tggcgattgc cggtttccag ccgcagttta ataaatgggt
    26641 agagttactg accgatcctg gtgttaacgg gatggcacgc gacgtggtgc tctctgatgc
    26701 gatgatgggc tatctccatt tcattgcaaa tattccggtc aaaggcactc gctggctata
    26761 tagcagtaaa ccttatgcgc ttgcaacgcc gccgctttcg gtgattaacc aatggcagca
    26821 ggcgctggat aaaggtcaat tgcctacgtt tgttgcagga ctggcaccgc agcatccgca
    26881 atatgcggtg atgcatgaat cgttactggc cttactcagt gacaccaaac cgtggcccca
    26941 actgaccggc aaagcaacgt tgcgcccagg gcagtggagt aacgacgtac cggcgttgcg
    27001 tgaaatattg caacgcacag gcatgttgga cggggggccg aaaattactc tacctggcga
    27061 tgacacgcca actgacgcgg tagtcagccc atccgctgtt actgttgaaa cagcagaaac
    27121 taagccgatg gataagcaaa cgacgtctcg tagtaaacct gcgcctgccg ttcgcgccgc
    27181 ctacgataat gaactggtgg aagccgttaa acgttttcag gcatggcaag gattgggggc
    27241 agatggtgct attggcccgg caacgcgtga ctggttaaac gtaacgcccg cccagcgtgc
    27301 tggcgtgttg gctctcaaca tccagcgatt gcgcttgctg ccaacagagc tttctaccgg
    27361 gatcatggtt aacattccgg cctattcgct ggtctactat cagaacggca atcaggtgct
    27421 ggattcgcga gtcattgtcg gtcgccccga tcgcaaaacg ccgatgatga gcagtgccct
    27481 taacaatgta gtggtaaacc cgccgtggaa cgtacctcca actctggcac gcaaagatat
    27541 tctgccgaaa gtgcgcaacg atccgggata tctcgaaagc catggctata cggtgatgcg
    27601 cggctggaac agcagagaag cgattgaccc atggcaggtt gactggtcta caatcacggc
    27661 ctcgaattta ccgtttcgct tccaacaggc tccaggccca cggaactcgc tggggcgcta
    27721 taaattcaat atgccgagtt cagaggccat ttatttgcat gacacgccga accacaatct
    27781 gttcaagcgt gatacacgcg cattgagctc aggctgtgta cgagtgaata aagcttccga
    27841 tctggcgaat atgctgttgc aggatgcagg ctggaatgac aaacgtattt ctgatgcgct
    27901 gaagcagggt gatacacgtt acgtcaatat tcggcagtcg attccggtga atctctacta
    27961 cctgacggcc tttgttggtg cagatggtcg tacccagtat cgtacagata tttacaatta
    28021 tgatctgcct gcgcgatcca gctcgcaaat cgtatcgaaa gcggaacaat taatcaggta
    28081 aatgaagtag ttcggggaaa tcagttgtcg taataattag catgattggg ggcaattctc
    28141 tgcagccccc gtcactgctg gggttgagtc atcttgacgt ctgctttacg ggcggttaag
    28201 gtgcctcttg tgcgccagaa gtgcatataa acgataacat tgacctgtag acttgattat
    28261 catggacaaa ttcgacgcta atcgccgcaa attgctggcg cttggtggcg ttgcactcgg
    28321 tgccgccatc ctgccgaccc ctgcgtttgc aacactctct accccacgcc cgcgcatttt
    28381 gacactcaat aatcttcata ccggagagtc aatcaaagcg gagtttttcg atggcagagg
    28441 ctatattcag gaagaattgg caaaacttaa ccattttttc cgcgattacc gcgcgaacaa
    28501 aataaagtcc atcgacccag gattattcga ccagttgtat cgcctgcaag ggttattagg
    28561 cacccgcaaa ccggtgcaac tcatttccgg ttatcgttcc attgatacca acaatgaact
    28621 acgcgcccgc agccgtggag tagcgaagaa aagctatcac actaaaggcc aggcgatgga
    28681 tttccatatt gaaggtatcg cgttaagcaa tattcgcaaa gccgcgttat ctatgcgcgc
    28741 aggtggtgta ggatattacc cacgtagtaa ctttgtgcat attgataccg ggccagcacg
    28801 gcactggtag taattgctta acgaaacagg ggcagtatga actatcgtat tattccggtc
    28861 accgcattct cccagaactg ttcattgatc tggtgtgaac aaacccgcct ggccgcactg
    28921 gtcgatcctg gcggcgatgc ggaaaaaatc aaacaggaag ttgacgccag cggcctgaca
    28981 ctgatgcaga tcctgctgac gcatggtcat ctggaccacg ttggcgcagc ggcggaactg
    29041 gcgcaacatt acggcgtgcc ggtttttggc ccggaaaaag aagatgagtt ctggctgcaa
    29101 ggcttgcctg cgcaaagtcg tatgtttggt ctggaagagt gccagccgct gacgccagat
    29161 cgttggctga acgaaggcga taccatcagc atagggaatg tgactttaca ggtgttacat
    29221 tgccctgggc atacgccggg tcatgtcgtg ttttttgatg atcgggcaaa gctgctgatt
    29281 tctggcgatg ttattttcaa aggcggagta gggcgcagtg acttcccgcg tggcgatcat
    29341 aatcaactga tttcttcaat caaagataaa ttgctgccac tgggtgatga cgtgacattt
    29401 attccgggtc acggaccatt atccacactt ggttatgaac gcctgcataa tcccttcctg
    29461 caagacgaaa tgcccgtctg gtaaggcaca cataaaaaag cccgcttttc agcgggcttc
    29521 attgttttta acgcttacag cactgccaca atcgcttcgc acagcggagc catgttatct
    29581 ggtgtcatcc cggccacgtt tacgcgacca gaagcaacag catacacgcc aaactcttcg
    29641 cgcagacgca gcacttgttc ttttgtcagg ccactgaagg agaacatgcc gttctgtttg
    29701 atgataaagc tgaagtcgcg gtttgcgcct ttttcctgca gcgtattgac gaacaactga
    29761 cgcatacgct gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaac
    29821 gcatcgttgc tcaggatggt ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag
    29881 ttagcgcgaa tcgccgcttt catttggctg aatgcgcgat caacggtttc actgtcggca
    29941 gcaaccagag tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa
    30001 ctggcaacaa tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct
    30061 tccagaccac gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca
    30121 acggagagtt gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggttg
    30181 tggcagcagc catggaacag cactacgtcg ccagcctgag cttcgttcag gctgttaatc
    30241 agtgcatcga agtcaagggt gtggttttcc gcatcataat aagcgtattc acgaacttcc
    30301 agatctgcag agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacact
    30361 cgcttaacgc tggtattttt tgccaggaaa tcggcagcta tgcgtagtgc gccagtgcca
    30421 cccggagtct gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac
    30481 agcagttcct gagtgcagcg accaaattca gggatgccgt caatgccgag gtaatttttg
    30541 gtggtttcat tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt
    30601 ttgcccgtct catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg
    30661 gcacgaaaca gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca
    30721 aacatgacga ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga
    30781 tttacaaaat tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt
    30841 gctctggaag gcataaaaaa aacaggacca aagtcctgtt ttttcggcat ttaacaaaga
    30901 ggtgtgctat tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc
    30961 cagtttgttg tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt
    31021 gttgaagtag taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc
    31081 ttctacgtct ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa
    31141 ctggtattgc gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tgatgtttgt
    31201 aaatttatta gtgatcggcg tagcgttacg cgtttcaccg tagttcgctg ccaggtagat
    31261 gttgttcgca tcatacttca gaccagtagc ccactgttca gcttttttac cgttgccaag
    31321 aagttgagct tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa
    31381 gccttcgtat tcgtagctga tagaaccgcc aacaccgtcg ccgttagagc ggcgtgcagt
    31441 gtcacgctcg tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa
    31501 gaagttggag ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct
    31561 gtatgcagta tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac
    31621 cacaccgtag ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa
    31681 tgccagacgc gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa
    31741 gttatattcc cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa
    31801 accaagacgg gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt
    31861 ggagaaataa tgcagaccga cagctttacc gtacagatct actttgttgc catctttgtt
    31921 atagatttct gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag
    31981 aatattgcgc ttcatcatta tttattaccc tcatggtttt ttttatgaca cctgccactg
    32041 ccgtcaataa tttctgtcaa taaaatttta cggaactatt gatgagagtt tggtgtcttt
    32101 atgtgtctgc aggcatcttt ccattcaaac taacgtttcg ctaccgtgaa agtgctacaa
    32161 agataaagat ttagtttcaa aaagaaaaaa tatgtaacta aaagtaaaaa ttaaggaact
    32221 ttgtgaacac cgtcatattt ccatagaggc gtgatgatat ttacagcaat ttttatctat
    32281 ttatatgatt tccttatatt taaattaact aaacggaaat tttgtttctg atggaaactt
    32341 tatcgacctg gcccaaaatc ctctttcaga catccagaat gccaaaaaaa tagtatgaaa
    32401 attgtgctat cagctaagaa aaaactaatc cgcaataaga atgcgggtta ttttttgtgc
    32461 gtaaatgttg caatgtaata ttccgtaaca ggatgatcgt tatggcgggg ttgcaaatag
    32521 attgattgac ttaacaaaca gataacttga cagaaaggat aaaaaagagc cagcgggtgc
    32581 tggctcttgt agacgctaaa ttagaagctg gcgttacgcg gagttcgtgg gaacggaatc
    32641 acatcacgca cgttttgcac accagttacg taagcaatca ggcgttcaaa accaagaccg
    32701 aaaccggaat gcggaacagt accgtagcga cgcagatcgc gataccacca gtaatcttct
    32761 ttgttcaggc ccatttccag catacgctcg tccagcacgt ccagacgctc ttcacgctgg
    32821 gagccaccaa tgatctcacc gatgcccgga gccagaacat ccatagccgc aacggtttta
    32881 ccgtcttcgt tgaggcgcat atagaacgct ttaatatctt tcggatagtt tttaaccact
    32941 accggtgctt taaagtgttc ttccgccaga taacgctcat gctcagaaga gagatcgacg
    33001 ccccagtaaa ccgggttttc aaacttcctg ccgcagtttt cgagaatggt tactgcttcg
    33061 gtgtaatcca cctgcgcgaa atcggcctca ataaagcgtt ccagacgtga aacggcatct
    33121 ttatctacgc gttcagcgaa gaatttcatg tcgtcagcgc gttcttcgag aaccgctttg
    33181 aagacatatt tcagcatggc ttcagccaga cccgcgatat cgttcaggtt agcaaacgcc
    33241 acttccggct ccagcatcca gaattccgcc aggtgacggc tggtgttgga gttttcagca
    33301 cggaaagtcg ggccgaaggt ataaattttg gataatgcgc aagcgtaggt ttcgccgttc
    33361 aactggccag atacggtcag gaaagactct ttaccaaaga agtctttgtc gaaatccact
    33421 ttgccctgat cgttacgcgg cagattttcc agatccagcg tagaaacgcg gaacatttcg
    33481 ccagcccctt cggtatcaga cgcagtaatc agtggcgttg aaacccagaa gaatccctgc
    33541 tcgttaaaga agcgatgcag cgcctgcgcc agcgtatggc gaacgcgcgc gacggcacca
    33601 atcaggtttg tgcgcggacg caggtgagcg acttcacgca gatactcaat gctgtggcgt
    33661 tttgccgcca tcgggtaagt gtctggatct tcaacccaac cagcaacttc aaccttgctg
    33721 gtctgaattt caaattgttg cccctggccc ggcgacgcca cgactttacc cgtcacaatg
    33781 accgagcagc cggtggtcag acgcaggacg tcttcattgt aattgggcag agaattattg
    33841 atgacagcct gtacaggatc aaagcaggaa ccgtcataaa cggcgaggaa ggagatgcca
    33901 gcttttgaat ctcggcgggt acgtacccat ccgcgtacgg tgacttcgct gtcaacggct
    33961 acgcggccct ggagtacgtc ggctacaggc acaacgctca taatattctc tctgttaata
    34021 gtcggaaaaa ataaacactt gtccacccgt aaatgggggg atacctatgt tacctggcat
    34081 ctgcaatcag acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa
    34141 aaagggagcc gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg
    34201 aacgctttgc gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg
    34261 tcagaaagtt tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg
    34321 ggttttacct gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg
    34381 cgggaagaga agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa
    34441 accagcgttt tactctgtgg atcaatttcc agcttttcat aatgtgcaat ggctttttca
    34501 ccccattcaa ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg
    34561 acaccgaaat cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt
    34621 tggtcgggat actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc
    34681 gggctgattt gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg
    34741 gaaagccgac gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga
    34801 cgcttaacga tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc
    34861 agatggaagc gcgacatatc aagaccagcg gttaacgccg agaagtcgac taatttgctt
    34921 tccagcgtgt cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt
    34981 tcactgataa ccgccagcaa aggaacctcc cagaggatga cttcacgcca cgggccgctt
    35041 aaacgaatat ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg
    35101 aactcgcgta accagttaag atagtcggcc ttaaagaaag gcagggcaga aagccactga
    35161 tattcatcat cctgcaggcg caggtgctgc atcgcctgaa cctgttcacg aatagcatcg
    35221 gcataaatac ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg
    35281 taataatgat gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac
    35341 gagtgcagaa caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga
    35401 gcgtttagta caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat
    35461 tgaaccccga tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta
    35521 taaaaatgta gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat
    35581 ttacgcaaca ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg
    35641 actcaacagc cacaagccaa ataccgtcac gattatcgtg cgccggatta ccagattact
    35701 gatattgact tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag
    35761 gctgtccgtc atggtgcatc agatgctccg ctttgtctca acggcgaaga cctcaaactg
    35821 gtttctgttc atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg
    35881 gtcatcagta atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg
    35941 gcgaatacgg cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa
    36001 gccgaaggtt tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt
    36061 accaccaaaa ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc
    36121 gttgcgcaag gcgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg
    36181 aaaccgtgct acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt
    36241 accacgcgtt ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat
    36301 cgcgcgccgt gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt
    36361 ggcctggagt atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc
    36421 gcaatggaga ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgat
    36481 accgccaccg acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat
    36541 aactggaccg gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt
    36601 ttaaccgtct tccgcgatca ggaattcagc tctgaccttg gttcccgcgc ggtaaaccgt
    36661 atcaataatg tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg
    36721 cacccaatcc gcccggacat ggtcattgag atgaacaatt tctacaccct gaccgtttac
    36781 gagaagggcg cggaagtgat tcgtatgatc cacactctgc ttggcgaaga aaacttccag
    36841 aaagggatgc agctctattt cgagcgccat gatggcagtg cagcgacctg cgacgatttc
    36901 gttcaggcga tggaagatgc gtcgaatgtc gatctctccc atttccgccg ttggtacagc
    36961 cagtccggta cgccggttgt gaccgtcaaa gacgactaca atccggaaac cgagcagtac
    37021 accctgacca tcagccagcg cacgccagcc acgccggatc aagcagaaaa acagccgctg
    37081 catattccgt ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa
    37141 ggcggtcatc cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt
    37201 gataatgtct acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa
    37261 ctggaatata agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat
    37321 ttttcccgtt gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc
    37381 cgtcatcagc aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg
    37441 gtgctgctcg atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct
    37501 gtcaatgaaa tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc
    37561 gaagcactca ctcgtactct ggcgactgaa ctggcggatg agctactggc tatttacaac
    37621 gcgaattacc agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt
    37681 aatgcctgcc tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc
    37741 aagcaatacc atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt
    37801 gctgcacagc tgccttgccg tgacgcgctg atgcaggagt acgacgacaa gtggcatcag
    37861 gatggtctgg tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg
    37921 ctggagacgg tgcgcggcct gttgcagcat cgctcattta ccatgagcaa cccgaaccgc
    37981 attcgttcgt tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat
    38041 gggagcggtt accagttcct ggtggaaatg cttaccgacc tgaacagccg taacccgcag
    38101 gtagcttcac gcctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag
    38161 gagaaaatgc gcgcggcgct ggaacagttg aaagggctgg aaaatctatc tggcgatctg
    38221 tacgagaaga tcaccaaagc actggcttaa taaatccccg aatggcggca atagcgccgc
    38281 cattcgggga ttttacccct gttttctcag gcgagtttca gattcaccac gctgcatcac
    38341 ccgttgcaac acttccgctt ccagctccgc taatcgcacc gaccctaacc ggcgcggacg
    38401 gggaatatcc accgtcaaat ccagaccaat ttttccctct tcaattaaca acacccgatc
    38461 agccattgct accgcttcgc tcacatcatg cgtcaccaac agtacggtaa aaccgtgctc
    38521 ctgccaaagt gacacaatca aatcctgcat ctcgagtcgc gttaaggcat ccagcgcacc
    38581 gagcggttca tcaagcaaca ataaacccgg tcgatgaatc aacgctcttg ccagcgccac
    38641 tcgctgtttc tgcccgccag aaagtgcggc aggccattcc ccggcgcgat tctccagtcc
    38701 taccgcagcc aacgcccgac gcgcggcatc gcgccactgg cctttaaggc ctaacccaac
    38761 gttatcaatc accgatttcc acggtagcag acgcgcatcc tgaaacatca ttcgcgtgtc
    38821 ttcctgaatt tcagccagcg gtgtggtgcc cgctaacaca tcgccagcgg ttggcgtttc
    38881 cagacctgcc agcaggcgca gcagggtact tttgccacca ccgctgcggc ccaccaccgc
    38941 cacaaactga cctgccggaa tatgtaaatc cagttggttc aggacgatat tttccgcgta
    39001 atgtttgctt actgcattga gcaacaatgg tgtgccctgg ttcagacgag cagtattcat
    39061 accgtggcct ccttcaaatg ataagccggg ttccagcgca accagaggcg ctctaacagc
    39121 tgcgcgctga cgtcagcaag tttgccgagc agggcgtaaa gaataatagc gactaccacc
    39181 acgtccgttt gcaaaaactc ccgcgcattc atcgccagat aaccaatgcc tgaattggca
    39241 gaaatggttt cggcaacaat cagcgtcagc cacatcaggc ccaacgcaaa acgcacgccg
    39301 accataattg agggcagggc accaggcagg atcacatgga taaacagcgg tatgccggat
    39361 aatccatagc tacgcgccat ctccaccaac ccgcgatcga tattacggat gccatgccag
    39421 gtgttgatat aaatggggaa tagcgtaccg agcgccacca gaaagatttt tgcggattca
    39481 tcaatgccaa accacaaaat caccagcggg atcagcgcca gatgcggcac gttgcgcaac
    39541 atctgaattg aggtatccag cagcctttct ccccagcgcg acagtccgct aatcagtccc
    39601 aaaatcaacc ccagcgagcc gccaattgaa aaaccaatca gcgcccgcca ggagctgatc
    39661 gccagatgct gccacaattc gccgctggct gagagcgtcc agaacgccat caccacccct
    39721 tccggtgagg gcaaaatacg cgtcgacaac cagccaaccg aggaggccag ttgccacacc
    39781 gtcacgatgc ccaccggtaa aaaccagggg gcaacgcgca ataaccactt cttcactggc
    39841 gttgccatta tcttcttcct tagctttgcg cgactctgcg agggataaaa tcattctcca
    39901 ccgcttcgcc ttgcggattc agcggctgcg gctggggaat ttccggaatg gcgacatcca
    39961 gatgtgggaa cagcaactcg ccgacccgat acgcttcttc cagatgcgga tagcccgaaa
    40021 gcacaaagct gtcgatgcca agcgcggcat attcgttgat tcgcgcagcg accgtaggac
    40081 catcgcccac cagcgccgtc ccggcaccgc cgcgcactaa gccaacgccc gcccataaat
    40141 tggggctgat ctccagattg tcgcgcttgc cgttatgtag cgctgccatt cgctgttgcc
    40201 ccacggaatc cgtgcgggcg aatgcggctt gtgctttggc gatagtctca tcatcaagat
    40261 gcgagattaa ccgctcggcg gcctgccacg cttcgtcgtt agtttcacga acaatcacat
    40321 gcagacgaat gccgaaacga attttgcgtc catgcgcggc agctttcgcc cgcacttgtt
    40381 cgattttctc tttaaccagt tccggcggtt cgccccaggt gaggtagaga tcaacctgtt
    40441 ctgccgccag ctcctgggcg acgtctgacg atccgccaaa gtaaagcgga ggatacggct
    40501 gttgaatcgg cgggaagagc agtttggctc cccgcacatg aatatgttta ccgttgaaat
    40561 cgacggtttc ccccagcaat aaacgccgcc agacctgggt aaattccgcc gaggcttcgt
    40621 agcgctcact atgatcaagg aacacgccgt cgcctgccag ttcttgtgga tcgctgcctg
    40681 tgaccaggtt aaacaacgca cgtccatttg agagacggtc aagcgtggcg gcctggcggg
    40741 cggcaacggt aggtgaggtt acgctgggac gcagggcgac aagaaacttc agccgctgcg
    40801 tcaccgggat catcgatgcg gcaaccagcc acgcatcttc gcaggagcgc cccgttggga
    40861 tcagcacacc ggtatagcca agacgatccg ccgcttgcgc aatttgttgc agataaccgt
    40921 gatcaaccgg gcgtgaacct tcttccgttc ccagataatg cccgtcacca tgggtcggta
    40981 aaaaccagaa catattcaga ctcataattg ttttccttcc agttgagtgg gctgccagat
    41041 gcgttggcga atatcgactt ttttcggcac cagacgattt tcataaaaca gatctgccgt
    41101 ttgctgctgt aaggcggcaa cctcggcgtt aaccggtttg atggtggtag gagggcgatg
    41161 atctaagtaa gaggcaatca ccggtgccgg taagcccatc gtttttgcca gtaaagcgat
    41221 actttgctcg cgctggctgc gggttaacgc atcggcctca ctaaaggttg ccagtacacc
    41281 ctgaataaaa gcgccgtttt tttctgcata ggggcgagct gccagataaa acgatccggt
    41341 ttgattgaga tcggtgccgt ctttcaacac ccgcacgccg ccctgtaata atgcagcgga
    41401 gtagtaggga tcccagatag cccaggcgtc aacgttacct tgctggaacg cggcgcgggc
    41461 atcagctggc gtcagataag tgggctggat gtcagtaaac ttaagtccgg cctggcgtag
    41521 tgcgcgcagt aaaagattgt gtgaactgga acctttctgg aacgcaactt tgtggccttt
    41581 aagatcggct gcggttttga tcgggctgtt ttctgccacc agaatcacct cggcttttgg
    41641 cttcggcggc tcgacgccca cgtacagcaa atcagccccg gcagcctggg cgaagattgg
    41701 cggaatatcc ccggtactgc cgagatcaat actgccaacg tttaacgcct caagcatttg
    41761 cgggccagcg gggaactcca cccacgagat ttttgattgc ggatagcgtt tttccagtaa
    41821 ctggtggctt tttgccaata ccataccaat actgcctttc tgatagccta tacgtaacgc
    41881 ttcaggcgag gattctgcag caaccacaat cgtagagacg ctaagcaatc ccaccagcgc
    41941 cagtttaatg atgttacgca tgggcattac ctcgcagaga atgaagatca ggaacctgaa
    42001 catcgcggcg gtgcaatgcc tgccagaaag tttctagcgc ggtatcaaga cgggtttgta
    42061 aattcggcgt gaactggggt ttgtgatggt aatcaattac ttgtgagtca tcggcaaaca
    42121 cgccgtgcag gatctcctgg gctttcagcg cacttaaaac tggcttaagg gcataatcga
    42181 ccgccagcag atgggccacc gtaccgcccg tcgccagcgg tagcaccact ttgccttgca
    42241 acgcacgttc tggcagcagg tcgagcaggg ttttcagcgc accggaataa gcggctttat
    42301 acacaggcgt ggcgacaatc agcccgtcag cctgttgcaa ctgttcggtg aaggtatgga
    42361 gtgccggact atcgaaacga gcataaagca gatcttccgc ggcgaagttt tgcagattcc
    42421 agtgataaac ctcaacatcc aggccattta gtttttcccg cgcatattcc agcaaggagc
    42481 tggagcgaga aggaaagcga ggactacccg ccagggtgat gacacgcata ctctctcctt
    42541 ataaccaatt gttcttgttt tgttaacatt gataacaatt cgctcagtct gtcggagaga
    42601 caagaaaatg ccaaatataa attttgtgta tctttttctg aaaataaact aaagaaaggg
    42661 ttttatatat cacgcatatt tatttattgg atagtcatta gatgttgaat ca
//
LOCUS       DAZFTN010000026        42540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFTN010000026 DAZFTN010000000
VERSION     DAZFTN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..199
                     /locus_tag="ACHKV2_001613"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHKV2_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(286..961)
                     /locus_tag="ACHKV2_001614"
                     /pseudo
     CDS             complement(286..961)
                     /locus_tag="ACHKV2_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1106..1714)
                     /gene="yiiX"
                     /locus_tag="ACHKV2_001615"
     CDS             complement(1106..1714)
                     /gene="yiiX"
                     /locus_tag="ACHKV2_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO6939517.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1774..2091)
                     /gene="metJ"
                     /locus_tag="ACHKV2_001616"
     CDS             complement(1774..2091)
                     /gene="metJ"
                     /locus_tag="ACHKV2_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO6939518.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2368..3528
                     /gene="metB"
                     /locus_tag="ACHKV2_001617"
     CDS             2368..3528
                     /gene="metB"
                     /locus_tag="ACHKV2_001617"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO6939519.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3531..5963
                     /gene="metL"
                     /locus_tag="ACHKV2_001618"
     CDS             3531..5963
                     /gene="metL"
                     /locus_tag="ACHKV2_001618"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO6939520.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6102..6236)
                     /locus_tag="ACHKV2_001619"
     CDS             complement(6102..6236)
                     /locus_tag="ACHKV2_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939521.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6312..7202
                     /gene="metF"
                     /locus_tag="ACHKV2_001620"
     CDS             6312..7202
                     /gene="metF"
                     /locus_tag="ACHKV2_001620"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO6939522.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7531..9711
                     /gene="katG"
                     /locus_tag="ACHKV2_001621"
     CDS             7531..9711
                     /gene="katG"
                     /locus_tag="ACHKV2_001621"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO6939523.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9805..10710
                     /gene="yijE"
                     /locus_tag="ACHKV2_001622"
     CDS             9805..10710
                     /gene="yijE"
                     /locus_tag="ACHKV2_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO6939524.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10737..11354)
                     /gene="yijF"
                     /locus_tag="ACHKV2_001623"
     CDS             complement(10737..11354)
                     /gene="yijF"
                     /locus_tag="ACHKV2_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO6939525.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11628..12731)
                     /gene="gldA"
                     /locus_tag="ACHKV2_001624"
     CDS             complement(11628..12731)
                     /gene="gldA"
                     /locus_tag="ACHKV2_001624"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO6939526.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12742..13404)
                     /gene="fsa"
                     /locus_tag="ACHKV2_001625"
     CDS             complement(12742..13404)
                     /gene="fsa"
                     /locus_tag="ACHKV2_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6939527.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13416..15917)
                     /gene="ptsP"
                     /locus_tag="ACHKV2_001626"
     CDS             complement(13416..15917)
                     /gene="ptsP"
                     /locus_tag="ACHKV2_001626"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6939528.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16226..17305
                     /gene="frwC"
                     /locus_tag="ACHKV2_001627"
     CDS             16226..17305
                     /gene="frwC"
                     /locus_tag="ACHKV2_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO6939529.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17320..17640
                     /gene="frwB"
                     /locus_tag="ACHKV2_001628"
     CDS             17320..17640
                     /gene="frwB"
                     /locus_tag="ACHKV2_001628"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6939530.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17691..19988
                     /gene="pflD"
                     /locus_tag="ACHKV2_001629"
     CDS             17691..19988
                     /gene="pflD"
                     /locus_tag="ACHKV2_001629"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6939531.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19954..20832
                     /gene="pflC"
                     /locus_tag="ACHKV2_001630"
     CDS             19954..20832
                     /gene="pflC"
                     /locus_tag="ACHKV2_001630"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO6939532.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20834..21175
                     /gene="frwD"
                     /locus_tag="ACHKV2_001631"
     CDS             20834..21175
                     /gene="frwD"
                     /locus_tag="ACHKV2_001631"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6939533.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21162..22013)
                     /gene="yijO"
                     /locus_tag="ACHKV2_001632"
     CDS             complement(21162..22013)
                     /gene="yijO"
                     /locus_tag="ACHKV2_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6939534.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22228..23961)
                     /gene="eptC"
                     /locus_tag="ACHKV2_001633"
     CDS             complement(22228..23961)
                     /gene="eptC"
                     /locus_tag="ACHKV2_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6939535.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24144..26795)
                     /gene="ppc"
                     /locus_tag="ACHKV2_001634"
     CDS             complement(24144..26795)
                     /gene="ppc"
                     /locus_tag="ACHKV2_001634"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO6939536.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27147..28298)
                     /gene="argE"
                     /locus_tag="ACHKV2_001635"
     CDS             complement(27147..28298)
                     /gene="argE"
                     /locus_tag="ACHKV2_001635"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO6939537.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28452..29456
                     /gene="argC"
                     /locus_tag="ACHKV2_001636"
     CDS             28452..29456
                     /gene="argC"
                     /locus_tag="ACHKV2_001636"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO6939538.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29464..30240
                     /gene="argB"
                     /locus_tag="ACHKV2_001637"
     CDS             29464..30240
                     /gene="argB"
                     /locus_tag="ACHKV2_001637"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO6939539.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30301..31674
                     /gene="argH"
                     /locus_tag="ACHKV2_001638"
     CDS             30301..31674
                     /gene="argH"
                     /locus_tag="ACHKV2_001638"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO6939540.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31941..32858
                     /gene="oxyR"
                     /locus_tag="ACHKV2_001639"
     CDS             31941..32858
                     /gene="oxyR"
                     /locus_tag="ACHKV2_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO6939541.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32841..34241)
                     /gene="sthA"
                     /locus_tag="ACHKV2_001640"
     CDS             complement(32841..34241)
                     /gene="sthA"
                     /locus_tag="ACHKV2_001640"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO6939542.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34571..35737
                     /locus_tag="ACHKV2_001641"
     CDS             34571..35737
                     /locus_tag="ACHKV2_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO6939543.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            35780..37096
                     /locus_tag="ACHKV2_001642"
     CDS             35780..37096
                     /locus_tag="ACHKV2_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO6939544.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            37200..37850
                     /gene="fabR"
                     /locus_tag="ACHKV2_001643"
     CDS             37200..37850
                     /gene="fabR"
                     /locus_tag="ACHKV2_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO6939545.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            37850..38209
                     /gene="yijD"
                     /locus_tag="ACHKV2_001644"
     CDS             37850..38209
                     /gene="yijD"
                     /locus_tag="ACHKV2_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO6939546.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38249..39349)
                     /gene="trmA"
                     /locus_tag="ACHKV2_001645"
     CDS             complement(38249..39349)
                     /gene="trmA"
                     /locus_tag="ACHKV2_001645"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO6939547.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39463..39653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            39718..41562
                     /gene="btuB"
                     /locus_tag="ACHKV2_001646"
     CDS             39718..41562
                     /gene="btuB"
                     /locus_tag="ACHKV2_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO6939548.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41507..42364
                     /gene="murI"
                     /locus_tag="ACHKV2_001647"
     CDS             41507..42364
                     /gene="murI"
                     /locus_tag="ACHKV2_001647"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO6939549.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9689 a        10579 c        11779 g        10493 t
ORIGIN      
        1 aaatatttcc agatggagaa aatattttaa tatataaagt aaatataatt aatgctagtt
       61 caaaaaagat gtattttttt atgaaagccc aacatcaacc cacaatgaca taatgggggg
      121 cgggggaatg tcatatgcct atgaatatga tgtaaaatat aatatatgtc atttgagaaa
      181 aaagagcaga atgaggtaaa aatctttggc tgtgtattca gtctggtaat catccaagga
      241 aagctcatca gaagggtgat gactccaatt agtagaacat atattttcga taaaagcacc
      301 gatgactttt tcatgaagtt ctacagaact cgagaaacag atggttctgc gggccagacg
      361 tttgattcgg gttctcaggg tcagattgtt acgctcaatg tgctgagtaa aaatcttacc
      421 agtcagatgc ttctcctttg gtaactctct agcgtaacta ccccattcgt cagtggtcaa
      481 catgccgatg gtaaaagggt aagcagcgcc agcaattcac gacaggtttc atcagtccgg
      541 gggccaaaag tgtaggccag aacaccacca gttttcgtgt tgtacacgta ccagagccag
      601 tgctgtcgcg ctttactgcc gacaaagctc cattgctgac taagtttgca gatgagtgcc
      661 acatcagcat gagcgaccgg ggatgacgtt attcttcgtg gcgagagttt tttaaagttc
      721 ggatgactgt attaatgccg attttcagcg tacgggcagt atagcgaacc ccggcaccgt
      781 tgaaagccat ttcagcgatt tggtctttaa ctcccggttt acggggctcg taacagtaac
      841 tgagctgaaa tacccggtga cagtcacggc cgcggaatcg atcatgtctt ttggggttct
      901 gcccgcgacg gtaaacctga acggactggc aacggggaca atgaaggtta actgtgacca
      961 tgaaacatcc ttaaaagcga gtattatatc cttaattcaa ctaattggag gcatcaccgt
     1021 ttaccggagc gacagcttgc ggaaaggtcg tgcaactaca aagcaacaaa cagactacgg
     1081 cgggaattat ttttttcatc atccattacc acgaaaacag cggccattct ttggcaaccg
     1141 tggtgagttg tggcgcatcg aaaacggctt gtggtgagac caccgtttcc tcaagcggaa
     1201 tatttttgcc gtagcgttct ttgagcttcg cctggaccag cgggttgctg agatcaaact
     1261 ctttcaactt ctgctgttcg cccacacgca ttcccagcgc attctgataa accttccaca
     1321 ccacttccga acagtactgg cgatcgtcgc tccatgagaa gctgaaatcg tacggtttgc
     1381 cgagataacg tttcgccgtt tgcgccagtt tttgctgttg ttcaacactc agtccgcctt
     1441 caacgcggcg aacaacgtat ttgccctttt caccatgggc aatccactgc ttgagcgggg
     1501 tgtatttcac cgggccgacc gcttcaaaaa cgtagggctt tttgttacgc atcaccagca
     1561 taccggtgtg gctgtaatcg gaatgggtcg ccagttggat cgctttactt tgcgatgagc
     1621 gagagatttg aaagatgatg tcgccagttt gcggctgcca ggcaaaggca ggcacagaaa
     1681 ccagcaggct gaggatcagt agcctgattt tcatcgttaa acgtccttgt atgaaaaaag
     1741 cggctggtta actccgcgcc gctcttttcc gctttagtat tcccacgtct ccgggttaat
     1801 ccccatctca cgcatgatct cttttgccgc ttccgggatt tcgtcgctgc gctctttacg
     1861 cagatcggca tcatccggca aaggttgccc ggtaaaggca tgcagaaacg cttcgcacag
     1921 cagctcgctg ttggtagcgt gacgcaggtt gttcacctga cgacgcgtgc gttcatcggt
     1981 gaggattttt aacaccttaa gaggaatgga aaccgtaatc tttttgactt gttcactctt
     2041 cttgccgtgc tcagcgtatg ggctgatata ttcgccgctc cattcagcca tgagatactt
     2101 aatcctcttc gtcaataaat tgagaccaga ccacacaatt tatgtgggta ctgaccgtaa
     2161 acccgcatag tttaccgtac aggcgttacc gtgacatcgt gtaatgcacc tgtcggcgtg
     2221 ataatgcata taattttaac ggctatttgg gatttgctca atctatacgc aaagaagttt
     2281 agatgtccag atgtattgac gtccattaac acagtgttta ctctggtgcc tgacatttca
     2341 ccgaccaagc ccagggaact tcatcacatg acgcgtaaac aggccaccat cgcagtgcgt
     2401 agcgggttaa atgacgacga acagtatggt tgcgttgtcc ctcccatcca tctttccagc
     2461 acctataact ttaccggatt taatgaaccg cgcgcgcatg attactcgcg tcgcggcaac
     2521 ccaacgcgcg atgtggttca gcgtgcgctg gcagaactgg aaggcggtgc tggtgcagta
     2581 cttactaata ccggcatgtc cgcgattcat ctggtaacga ccgtcttttt gaaacctggc
     2641 gatctgctgg ttgcgccgca cgactgctac ggcggtagct atcgcctgtt cgacagcctg
     2701 gcgaaacgcg gttgctatcg cgtgttgttt gttgatcaag gcgatgaaca ggcattacgg
     2761 gcagcgctgg cagaaaaccc caaactggta ctggtagaaa gcccaagtaa tccattgtta
     2821 cgcgtcgtgg atattgcgaa aatctgccat ctggcaaggg aagtcggggc ggtgagcgtg
     2881 gtggataaca ccttcttaag cccggcatta caaaatccgc tggcattagg tgccgatctg
     2941 gtgttgcatt catgtacgaa atatctgaac ggtcactctg acgtggtggc tggcgtggtg
     3001 attgctaaag acccggacgt tgtcactgaa cttgcctggt gggcaaacaa tattggcgtg
     3061 acgggcggcg cgtttgacag ctatctgctg ctacgcgggt tgcgaacgct ggtgccgcgt
     3121 atggagctgg cgcagcgcaa cgcgaaggcg attgtgaaat atctgcaaac ccagccgttg
     3181 gtgaaaaaac tgtatcaccc gtcgctgccg gaaaatcagg ggcatgaaat tgccgcgcgt
     3241 cagcaaaaag gctttggcgc aatgttgagt tttgaactgg atggcgatga gcagacgctg
     3301 cgccgtttcc tgggcgggct gtcgttattt acgctggcgg aatcattagg gggagtggaa
     3361 agtttaatct ctcacgccgc aaccatgaca catgcaggca tggcaccaga agcgcgtgct
     3421 gccgccggga tctccgagac gctgctgcgt atctccaccg gtattgaaga tggcgaagat
     3481 ttaattgccg acctggaaaa tggcttccgg gctgcaaaca aggggtaaaa atgagtgtga
     3541 ttgcgcaggc aggggcgaaa ggtcgtcagc tgcataaatt tggtggcagt agtctggctg
     3601 atgtgaagtg ttatttgcgt gtcgcgggca ttatggcgga gtactctcag cctgacgata
     3661 tgatggtggt ttccgccgct ggtagcacca ctaaccagtt gattaactgg ttgaaactaa
     3721 gccagaccga tcgtctctct gcgcatcagg ttcaacaaac gctgcgtcgc tatcagtgcg
     3781 atctgattag cggtctgcta cccgccgaag aagccgatag cctcattagc gcttttgtca
     3841 gcgatcttga gcgcctggcg gcgctgctcg acagcggtat taacgacgca gtgtatgcgg
     3901 aagtggtggg ccacggcgaa gtgtggtcgg cgcgcctgat gtctgcggtg cttaatcaac
     3961 aagggctgcc agcggcctgg cttgatgccc gcgagttttt acgcgctgaa cgcgctgcgc
     4021 aaccgcaggt tgatgaaggg ctttcttacc cgttgctgca acagctactg gtacaacatc
     4081 cgggtaaacg tctggtggtg accgggttta tcagccgtaa caacgccggt gaaacggtgc
     4141 tgctggggcg taacggttcc gactattccg cgacacaaat cggtgcgctg gcgggtgttt
     4201 ctcgtgtaac catctggagc gacgtcgccg gggtatacag tgccgatccg cgtaaagtga
     4261 aagatgcctg tctgctgcca ttgctgcgtc tggatgaagc cagcgaactg gcgcgtctgg
     4321 cggctcccgt tcttcacgcc cgtactttac agccagtatc cggtagcgaa atcgacctgc
     4381 agctgcgctg tagctacacg ccggatcaag gctccacgcg cattgaacgc gtgctggcct
     4441 ccggtactgg tgcgcgtatt gtcaccagcc acgatgatgt ctgcttgatt gagtttcagg
     4501 tgcccaccag ccaggatttc aaactggcgc ataaagagat cgaccagatc ctgaaacgtg
     4561 ctcaggtgcg cccactggcg gttggcgtgc ataacgatcg ccagttgctg caattttgct
     4621 acacctcaga agtggccgac agtgcgctga aaatcctcga cgaagcggga ttacctggcg
     4681 aactgcgcct gcgtcagggg ctggcgctgg tggcgatggt cggggcgggt gtcacccgta
     4741 acccgctgca ttgccaccgc ttctggcagc aactgaaagg ccagccggtc gagtttacct
     4801 ggcagtccga tgacggcatc agcctggtgg cagtactgcg caccggcccg accgaaagcc
     4861 tgattcaggg cctgcatcag tccgtcttcc gcgcagaaaa acgcatcggc ctggtattgt
     4921 tcggcaaagg caatatcggt tcccgctggc tggaactgtt cgcccgcgag cagagcacgc
     4981 tttcggcgcg taccggcttt gaatttgtgc tggcaggcgt ggtggacagc cgccgcagtc
     5041 tgttgagtta tgacgggctg gacgccagcc gcgcgttagc cttcttcaac gatgaagcgg
     5101 ttgagcagga tgaagagtcg ttgttcctgt ggatgcgcgc ccatccgtat gatgatttag
     5161 tggtgctgga cgttaccgcc agccagcagc tggctgacca gtatcttgat ttcgccagcc
     5221 acggtttcca cgtcatcagt gccaacaaac tggcgggagc aagcgacagc aataaatatc
     5281 gccagatcca cgacgccttc gaaaaaaccg ggcgtcactg gctatacaac gccaccgtcg
     5341 gtgcgggctt gccgatcaac cataccgtgc gcgatctgat cgacagcggc gatactattt
     5401 tgtcgatcag tgggatcttc tccggcacgc tctcctggct gttcctgcaa ttcgacggta
     5461 gcgtgccgtt taccgagctg gtggatcagg cgtggcagca gggcttaacc gagcctgatc
     5521 cgcgtgacga cctctccggc aaagacgtaa tgcgcaagct ggtgattctg gcgcgtgaag
     5581 cgggttacaa cattgaaccg gaccaggtac gtgtggaatc gctggtgcct gctcattgcg
     5641 aaggcggcag catcgaccat ttctttgaaa atggcgatga acttaacgag cagatggtgc
     5701 aacggctgga agcggcccgc gaaatggggc tggtgctgcg ctacgtggcg cgtttcgatg
     5761 ccaacggcaa agcgcgtgtg ggcgtggagg cggtgcgtga agaccatccg ctggcatcac
     5821 tgctaccgtg cgataacgtc tttgccatcg aaagccgctg gtatcgcgac aacccgctgg
     5881 tgatccgtgg gcctggcgcg gggcgcgacg tcaccgccgg ggcgattcag tcggatatca
     5941 accggctggc acagttgttg taatttcaga aatttaataa tgcccggtac tcatgttttc
     6001 gggtttatgg tttctaatga aatatattga attatcatag gattaggccg gatgaagcgt
     6061 ttacgacgaa tccggcaaga agcaataagt acatggttag tttatatttg cagtccggtt
     6121 tgctttgcat accggatttt ctttttctta ccatcctgaa gttttttcat cttccctgat
     6181 ttttcctcac catcattggt catttttcgg ttgacgccct tcggcttttc cttcatcttt
     6241 acatctggac gtctaaacgg atggatgtgc acaacacaac atataactac aagcgattga
     6301 tgaggtaagg tatgaacttt tttcacgcca gccagcggga tgccctgaat cagagcctgg
     6361 cagaagtcca ggggcagatt aacgtttcgt tcgagttttt cccgccgcgt accagtgaaa
     6421 tggagcagac cctgtggaac tccatcgatc gccttagcag cctgaaaccg aagtttgtat
     6481 cggtgaccta tggcgcgaac tccggcgagc gcgaccgtac gcacagcatt attaaaggca
     6541 ttaaagatcg cactggtctg gaagcggcac cgcatcttac ttgcattgat gcgacgcccg
     6601 acgagttgcg caccattgca cgcgactact ggaataacgg tattcgtcat atcgtggcgc
     6661 tgcgtggcga tctgccgccg ggaagtggta agccagaaat gtatgcttct gacctggtga
     6721 cgctattaaa agaagtggca gatttcgata tctccgtggc ggcgtatccg gaagttcacc
     6781 cggaagcaaa aagcgcccag gcggatttgc ttaacctgaa acgcaaagtc gatgcaggag
     6841 ccaaccgcgc gattactcag ttcttctttg atgtcgaaag ctacctgcgt tttcgtgacc
     6901 gctgtgtgtc ggcgggcatt gatgtggaaa tcattccggg catattgccg gtatctaact
     6961 ttaaacaggc gaagaaattt gccgacatga ccaacgtgcg tattccggcg tggatggcgc
     7021 aaatgtttga cgggctggat gacgatgccg aaacccgcaa gctggtgggc gcgaatattg
     7081 ccatggatat ggtgaagatt ttaagccgtg aaggagtgaa agatttccac ttctatacgc
     7141 ttaaccgcgc tgaaatgagt tacgcgattt gccatacgct gggggttcga cctggtttat
     7201 aaatagtgtg gcttttatga aaatcacaca gtgatcacaa attttaaaca gagcacaaaa
     7261 tgctgcctcg aaatgagggc gggaaaataa ggttatcagc cttgttttct ccctcattac
     7321 ttgaaggata tgaagctaaa accctttttt ataaagcatt tgtccgaatt cggacataat
     7381 caaaaaagct taattacgat caatttgatc tacatctctt taaccaacaa tatgtaagat
     7441 ctcaactatc gcatccgtgg attaattcaa ttataacttc tctctaacgc tgtgtatcgt
     7501 aacggtaaca ctgtagaggg gagcacattg atgagcacgt cagacgatat ccataacacg
     7561 acagccactg gcaaatgccc gttccatcag ggcggtcacg accagagcgc gggggcgggc
     7621 acaaccactc gcgactggtg gccaaatcaa ctccgtgttg acctgttaaa ccaacattct
     7681 aatcgttcta acccgctggg tgaggacttt gactaccgca aagaattcag caaattagat
     7741 tactacggcc tgaaaaaaga tctgaaagcc ctgctgacag aatctcagcc gtggtggcca
     7801 gccgactggg gcagctacgc cggtctgttt attcgtatgg cctggcacgg cgcggggact
     7861 taccgatcaa tcgatggacg cggtggcgcg ggtcgtggtc agcaacgttt tgcaccgctg
     7921 aactcctggc cggataacgt aagcctcgat aaagcgcgtc gcttgttgtg gccaatcaaa
     7981 cagaaatatg gtcagaaaat ctcctgggcc gacctgttta tcctcgcggg taacgtggcg
     8041 ctggaaaact ccggcttccg taccttcggt tttggtgccg gtcgtgaaga cgtctgggaa
     8101 ccggatctgg atgttaactg gggtgatgaa aaagcctggc tgactcaccg tcatccggaa
     8161 gcgctggcga aagcaccgct gggtgcaacc gagatgggtc tgatttacgt taacccggaa
     8221 ggcccggatc acagcggcga accgctttct gcggcagcag ctatccgcgc gaccttcggc
     8281 aacatgggta tgaacgacga agaaaccgtg gcgctgattg cgggtggtca tacgctgggt
     8341 aaaacccacg gtgctggtcc gacatcaaat gtaggtcctg atccagaagc tgcgccgatt
     8401 gaagaacaag gtttaggttg ggcgagcact tacggcagcg gcgttggcgc agatgccatt
     8461 acctctggtc tggaagtggt ctggacccag acgccgaccc agtggagcaa ctatttcttc
     8521 gagaacctgt tcaagtatga gtgggtacag acccgcagtc cggctggcgc aatccagttc
     8581 gaagcggtag acgcaccgga aattatcccg gatccgttcg atccgtcgaa gaaacgtaaa
     8641 ccgacgatgc tggtgaccga cctgacgctg cgttttgatc ctgagttcga gaagatctct
     8701 cgtcgtttcc tcaacgatcc gcaggcgttc aacgaagcct ttgcccgtgc ctggttcaaa
     8761 ctgacgcaca gggatatggg gccgaaatct cgttacatcg ggccggaagt accgaaagaa
     8821 gatctgatct ggcaagatcc gctgccgcag ccgatctaca acccgaccga gcaggacatt
     8881 atcgatctga aattcgcgat tgcggattct ggtctgtctg ttagtgagct ggtatcggtg
     8941 gcctgggctt ctgcttctac cttccgtggt ggcgacaaac gcggtggtgc caacggtgcg
     9001 cgtctggcat taatgccgca gcgcgactgg gatgtgaacg ccgcagccgt tcgtgctctg
     9061 cctgttctgg agaaaattca gaaagagtct ggtaaagcct cgctggcgga tatcatcgtg
     9121 ctggcaggtg tggttggcgt tgagaaagcc gcaagcgccg ctggtttgag cattcatgta
     9181 ccgtttgcgc cgggtcgcgt tgatgcgcgt caggatcaga ctgacattga gatgttcgaa
     9241 ctgctggagc caattgctga cggtttccgt aactatcgcg ctcgtctgga tgtttccacc
     9301 accgagtcac tgctgattga taaagcccag caactgaccc tgaccgcgcc ggaaatgacc
     9361 gcgctggtgg gcgggatgcg tgtactgggt gccaacttcg atggcagcaa aaacggcgtc
     9421 ttcactgacc gcgttggcgt attgagcaat gacttcttcg tgaacttgct ggatatgcgt
     9481 tacgagtgga aagcgaccga cgaatcgaaa gagctgttcg aaggccgtga ccgcgaaacc
     9541 ggcgaagtga aatacactgc cagccgtgcg gatctggtat ttggttctaa ctctgtcctg
     9601 cgtgcggtgg ctgaagttta cgccagcagc gatgctcacg agaagtttgt taaagacttc
     9661 gtggcggcat gggtgaaagt gatgaacctc gaccgtttcg acctgctgta atctgacctt
     9721 cttcagcgac tgcctttcag gcagtcgctg aagtttcttt accggcgtat agtgtccaca
     9781 ggaaaactac acactggatc tctcatgtct gccgcaggaa agagcaaccc actggcaatc
     9841 agtggcctgg ttgtgctcac acttatctgg agttatagct ggattttcat gaagcaagtc
     9901 accagctaca tcggtgcctt cgactttacc gccttacgct gcattttcgg cgctctcgtt
     9961 ttattcatcg tccttttatt acgtggtcgc ggaatgcgcc cgacaccgtt taaatacacc
    10021 ttagccattg ccctgttaca aacctgcggg atggttggcc tggcacagtg ggcgctggtc
    10081 agcggcggag cggggaaggt ggcgatcctg agctatacca tgccgttctg ggtggtgatt
    10141 ttcgccgcgg tgtttctcgg tgaacgcctg cgacgtgggc aatatttcgc gattctgatt
    10201 gccgctttcg gcttattttt ggtgttgcag ccgtggcaac tcgatttctc ttcgatgaaa
    10261 agtgccatgc tggcaatcct ctccggtgtt agttgggggg cgagcgcgat tgttgctaaa
    10321 cgtctgtatg cccgtcatcc gcgcgtggat ttattgtcgt taacatcctg gcagatgctc
    10381 tatgcggcgc tggtgatgag tgtggtcgct ttactggtgc cgcaacgtga aattgactgg
    10441 cagcccactg tgttctgggc tctggcctac agtgcgattc tggcgacggc gttggcgtgg
    10501 agcttatggc tgtttgtgct gaaaaacttg cctgccagta ttgccagctt aagcacactg
    10561 gccgttcccg tttgcggcgt actcttttcc tggtggctgc tcggtgagaa tccgggggcc
    10621 gttgaaggta gcggtattgt gctgattgtg ctggcactgg cgctggtgag ccgtaagaaa
    10681 aaagaagccg ttagtgtaaa aaggatctga attttttctt catgtggggc gatcgcttat
    10741 ttaacaaaat aacgataatg ccccaccatg cgccagctaa acagcacatc ttcttcctgc
    10801 gcacctgcgc caatgttatg tatcaccagc ggcgtaccgt cgcgggcgaa gccatctgaa
    10861 accaccccaa tatgtgccag cccgttatct aatcgccagg agacaatatc gcccgcttga
    10921 tagtcactgg ggttcttgct gatggggcgt gttttatcgt ggcgggtaaa ccaggtttcc
    10981 agattaggca cccgacggtg atcgatgttg ctgtccgggc gctttaactg ccatttttgc
    11041 gggtactcgg caaaattctt cgccatatct tcgtgcacca gtttctgcaa atcgaccttc
    11101 tggctgcgca atgcacggat caccacatcg gaacataccc cgcgttcttg cgggacatca
    11161 ccgccaggat aggtaagctg cacatacgcc ggatcgtaaa atagcgtgct gccgatttgc
    11221 tgtctggcac cgtctgcgat ggcaaggttg gtattggcct ggatttgtac cacggttggt
    11281 ggaatggcgg gagattttaa ggagtagctg gtaaatgccg ttagcaggct gaccagcgcc
    11341 agtgaagctt tcatctgttt cgtccgtgaa aataagagtc acagattcga ccttccgggc
    11401 taacgattgt tctgtagaga aaatgtccag aagcggataa aaaattcctg aacaacgtgg
    11461 gagtttcaga ttactggcac agagtgccgg atgcggcgtg aacgtcttat ctggcctaaa
    11521 aagcacgcaa attcaatata ttgcagagat tgcgtaggcc tgataagcgt agcgcatcag
    11581 gcaattttgc gttcaaactc ccggacaagc cgggagtttg gagtaggtta ttcccactct
    11641 tgcaggaaac gctgaccgta ctggtcggct accagcagag cggcgtaaac ctgatctggc
    11701 gtcgcgccgc caggcatgtt gtggatggtt tcaccttctg cacatgccgc ttctgccaca
    11761 attcgcattt tcgccgggac atcttcttta atatccagtt gagcgagagt tattggcaaa
    11821 cctaccgcat ggctaagcgc agctacggtt tcgatttcct caaccggcga gttttccagt
    11881 accagctgcg tcagtgtacc aaatgccact ttttcaccgt gataatagtg atgggcatca
    11941 ggaatggcag tcaggccatt atgcaccgcg tgcgctgccg ccagaccacc gctttcaaag
    12001 ccaacaccgc ttaaataagt gttcgcttca atcacgcgtt ccagtgccgg agtcactaca
    12061 tgctgttcgg cggcgagcat cgctttttcg ccttcttcca gcaaggtgtt gtagcacagt
    12121 tcagccagcg ccagcgccgc ctgagtgcac ttgccgcccg ccatggtggt cgcaccgcta
    12181 cgagagcagg cacgcgcttc aaaccaggtc gccagcgcat cgccgatacc cgccgctaac
    12241 agacgcgcag gtgcgcccgc gacgatttta gtatcgacaa tcaccatatt cgggttattt
    12301 ggcagcagca gatagcggtc aaattcacct tcgtcagtgt agataacgga aagagcgcta
    12361 caaggtgcgt cggttgaagc aatggtcggg gcaatggcga ccggaacacc catgaaatgt
    12421 gccagtgctt tggctgtatc gagggttttt ccgccaccga taccgagaat tgcgccacac
    12481 tgcgcagtct ccgcgatgcc acgcagacgg tcgatctcat tttgcgaaca ttcaccgcca
    12541 aacggcgcaa tctctactac cagtccagca tctttaaagc ttttctcgac agtggattga
    12601 gcaaaaccta aaacaaattt gtcacccacc actaaccagc gttctgccag cggcttcagg
    12661 tattcgccca gacgattaat cacatcagcg ccctggatgt atttacccgg tgattgaata
    12721 atgcggtcca taattgctcc tttagagatg agtagtgcca aatgcggcat tccagtcgtg
    12781 ttcgaacttc tctatagctg actctaccgc aggggtgttg agcatttgtt gcgctacatc
    12841 taagggcagg gtgatggatt cacatcctgc cagtaaacag tccagcgcct gacgcggcgt
    12901 tttaaagctg gccgccagca ccatgctttc tggtgcgtgc atttctaaca gcgcttgcag
    12961 ctcctgaacc gtacgaatgc cgtccccgcc ctgggcatcc acgcggttaa catacggagc
    13021 aacgtatttt gcccctgcca gtgcggctaa taacccttgt gcggcgctat atacagcagt
    13081 gccaagtgta gtaataccct cttttttcag tattttaatt gctgccagac cttcggaagt
    13141 caccgggatt ttcaccacaa tgcccggaat ggcatcgcgc agacgcttcg cttcttccac
    13201 catcccctgc gcgtcgcggc tcatggtctg agcaaacaga atgccctcat caccaatcgc
    13261 tttttgcaga cgcggcagca cttcccatat ggactccttg ctggcagcga taatgctcgg
    13321 gttagttgtc accccggcaa tcgggaatat gtgtgccaga cgttcgactt ctgcgacgtt
    13381 agcggtgtcc agatacagtt ccatgatgtt tccttttaca gttccagttc atgttgcagc
    13441 aggctggcga tagcgtcggc agaggcggcg ttaaccagcg cgttacggaa ttcttcgtgc
    13501 atgatgcggc gagcgaggcg cgagaaaatg cgcatatgct gatcgcccgc agcgtgtttg
    13561 ttcagggtta acataatgat gaattgcgct tcatcatcgc cccagcgcac cggcgcttgc
    13621 agacgcgcta cgctgatggt ggattgctca atgtgttctg atttgctgtg gggaatggca
    13681 aaactaaagc ccagaccggt agagaaaacg gcctcgcgcg cccacaagtc ggcttccagt
    13741 ttacgcggat agcggcagcg gcccgccagc agcaggttat cggtcatgcc tttgagcact
    13801 tcttctttgc tgcgccagtc gctttccagt gtgatgcact cggcggtgac cagcggtgcg
    13861 tcttgttggg tcatgcggaa ttgcgccagc aggtgttcca cttccagcga agtacggcag
    13921 gccattgcct ggttgagcaa cttgcggcac tcacggctat caagttgcgc catccgcgct
    13981 ttcgccgccg gaattgatgg tgcgctcatg ctgagttcat ccagccctaa gccgaccagc
    14041 aacggcagca cggaaccttt cgctcccagc tcaccgcaca gaccaatcca tttgccctgg
    14101 cgatgcaccg cttgcacggc gtaatcgagc gcccgcaaga atgccggatt caggctgttg
    14161 tagtgacgag taaccttagc gttatcgcga tccactgcca gcagatattg cgtcaggtca
    14221 ttactaccaa tactaaagaa atcaatctct tcgcagcatt gatcgatgat gaacatcacc
    14281 gacggcactt ccagcatgat gccgagctgg atcttctcat caaacggaat gtgttcgtta
    14341 cgcagttgct gtttggcttc cgccagtttt tctttcaccc ataagatctc ttccattgag
    14401 gagatcatcg ggatcatgat tttcaggctg ccgtgagcag aggcacggag gatcgaccgt
    14461 agttgtgtgg tgaacagcga ggcgtattct tcataaatac gcaccgcgcg ataaccgagg
    14521 aacgggttag cttctgccgg aatgttcaga taatcaacgg gtttgtcacc gccaatatcc
    14581 atagtgcgca caataatgct gcgtccgttg gcggactcca gcgcttgaca aaaaatgttg
    14641 tacaactcgc tttcgcccgg tgcgctggtg cgatccatat agagcatttc agtgcggaac
    14701 aaaccgacgc cttccgcacc attgccgaat gcggcctgcg cttccacgga gtgagcgatg
    14761 ttagcggcaa tttcaatgcg gataccgtca gcggtacggg cttgttgggt cagccagaca
    14821 cgctgttgct cacgcagggc gtcctgtacg cgggcttctt gctgataata acgagctacg
    14881 gcttcccctg gctcaaccac aatcgccccg gcgttgccgt cgatataaat cgtttgatgc
    14941 tgccacggag taagggcatc aatatctaca ccaaccagtg tcggaatgtt gaacgaacgg
    15001 gcaagaatta cggtatgtga ggtggtgccg ccacttttca gtaacagacc tttgaggtga
    15061 tttttatcca gttcgaggaa ctggctgggg gttagttcat cagccataca aatggcgggc
    15121 tgcgtcagtt tgcccggtgc cgggaagcgt tgctcaccgt agatttgctg aagcagctgg
    15181 aagcagacat cgcgtacgtc cagggcacgt tcttgcaggt agctgctgct ggaacgggaa
    15241 aactcttcac aaaagtgatt cgcactggcg acaatcgctt cggcgcagct taatccggcg
    15301 ctgacgcctg ccagtaaatg ttcgtgcagg gaagtatcgc cagccaggga tcggtgcgct
    15361 tccagaatcg cgctggtggc accgtcgcta tccagcagac gaaactcaat gtttttcagt
    15421 accagcgtca ggccgttttc cagtgcggat tgctcggcgt caacgtcttt ggctgcggga
    15481 agattaccca gcgcattgag atctaaagaa gagatcggcg tcagaatgcc gcctgcgcta
    15541 ccgctgcaca cggtgcgggc gcggataatt tgcggattca gattggtcag tgaaaccggc
    15601 agtggttcca gttcgtcaga tttaacttcc gccagcggcg catcgcagtg ggggaattca
    15661 tcgcgcagcc attggcttaa acgctggtgc gcttcctgtt cgtcggcccc ggaaatcagt
    15721 aactggcagt tatcgcccgc cagcgtgtcg gtgccaatca gcgcaagggc gcttttggcg
    15781 ttgcccttgc ggtcagtgcg caggttatgc cactcaattt gtgatgaaaa agtattacac
    15841 agcgtttcaa cgtggcttgc cggacgtgca tgtacgccgt taggtagctc acaaataaat
    15901 tccacaatca gggccattgc ctctctcccg gtacggtttt ctgtttacag gataagagcg
    15961 cacggcaacg gcctgccatg tgacaaatct gccaaaagct ggacaaatgt aatgtaaccg
    16021 tcaatttgcg acgcgtctca caagacgccg ttttgcggca tgcttccgga ttatcgcaag
    16081 ttatgaggcg gatcgcattt ttgtactgat attacaaaag tccagtaaat ggccttttta
    16141 tccactgttt gctccgcctg cgattgccta ttgtttcctc catcaaaaat atggacatgg
    16201 gctttcgcaa gcccgggagc agcttatgaa tgagttggtg cagatcctga aaaatacccg
    16261 tcagcattta atgacgggcg tttcacacat gattcccttc gtggtatcgg gcggtatttt
    16321 gctggcggtt tccgtcatgt tgtatggcaa aggcgcagtg ccggatgccg tagccgatcc
    16381 gaatctgaaa aaactgtttg atatcggcgt tgcaggtttg acgctgatgg tgcctttcct
    16441 cgccgcttac atcggttact ccattgcaga gcgttctgcg ctggctccgt gcgctatcgg
    16501 tgcctgggtt ggtaacagct ttggtgcggg cttctttggt gcactgatcg ccgggattat
    16561 cggcggcatc gtggtgcatt acctgaagaa aattccggtg cataaagttc tgcgctcggt
    16621 gatgcccatc ttcatcattc cgatcgtcgg cacactgatt accgcaggca tcatgatgtg
    16681 gggtctgggc gagcctgtag gggcgttgac caacagcctg actcagtggc ttcaggggat
    16741 gcagcagggc agcattgtta tgctggcggt gatcatgggt ctgatgctgg cgttcgatat
    16801 gggcggtccg gttaacaaag tggcctatgc cttcatgctg atttgcgttg ctcagggtgt
    16861 ttataccgtg gtggccattg ctgccgtggg tatttgtatc ccgccgctgg ggatggggct
    16921 ggcgacgctg attggtcgta aaaatttctc cgcagaagag cgcgaaaccg gtaaagcggc
    16981 gctggtgatg gggtgcgttg gggttactga aggggcgatt cctttcgccg cagccgatcc
    17041 gttacgtgta attccttcca tcatggttgg ttcagtttgt ggtgcagtaa ctgcggcgct
    17101 ggtcggtgcg cagtgctatg caggctgggg tggtctgatt gtgctgccgg tggttgaagg
    17161 taagttgggt tatatcgcag cagtggctgt cggggcggtt gtgacggcag tctgcgtgaa
    17221 cgtgctgaaa agtctggcgc gtaaaaatgg atcttctact gatgaaaaag aagacgacct
    17281 ggatttggat tttgaaatta attaattgag gaacgagcca tgacgaaaat tattgcagta
    17341 accgcgtgtc catctggtgt tgcccatact tatatggctg cagaggcgct ggaaagtgcc
    17401 gcgaaagcga aaggctggga agtgaaagta gaaacccagg gctcgattgg tctggaaaac
    17461 gaactgactg cggaagacgt ggcgagcgcc gatatggtga ttctgactaa agatatcggc
    17521 atcaagtttg aagagcgttt tgctggcaag accatcgtgc gcgtcaacat cagcgatgcg
    17581 gtaaaacgtg ctgatgccat tatgagcaaa attgaagctc acctggcgca aaccgcttaa
    17641 gttcttctcc cgctcgcaag ggcgggttcg ctttcccaca ggagttcctc atgacgaatc
    17701 gtatctctcg cctcaaaact gcactgtttg ccaatacccg tgaaatctcg ctggagcgtg
    17761 cgctgcttta taccgccagc catcggcaaa ccgaaggcga accggtgata ttgcgccggg
    17821 cgaaagcaac agcgtatatc cttgaacatg ttgaaatttc gattcgtgat gaagaactga
    17881 ttgccggtaa ccgcaccgta aaaccgcgcg ccgggattat gtcgccggaa atggaccctt
    17941 actggctgct gaaagagctg ggtcaattcc cgacgcgtcc gcaggaccgt tttgctatca
    18001 gcgaagaaga taaacgtatc taccgcgaag agttgttccc gtactgggaa aaacgttcga
    18061 tgaaagattt catcaacggg cagatgacgg atgaagtaaa agccgcgact agcacgcaga
    18121 ttttcagcat caaccagacg gataaaggcc aggggcacat tattattgat tacccgcgcc
    18181 tgctgaatca cgggctgggg gagctggtgg cacagatgca gcaacattgt cagcaacagc
    18241 cggagaatca cttttatcag gcagcgctgt tactgctcga agcctcgcag aaacacattt
    18301 tgcgttacgc cgaactggcg gaaacgatgg cggcaagctg cactgatgcc cagcgtcgcg
    18361 aagaactgtt gactattgcg gagatctccc gccataacgc cgaacataaa ccgcagacgt
    18421 tctggcaggc gtgccagtta ttctggtaca tgaacatcat tctgcaatac gaatccaacg
    18481 ccagttcgct gtctttaggg cgcttcgacc agtatatgtt gccgttctat caggcatctt
    18541 taacccaggg cgaagatccg gcgttcctga aagaactgct cgaatcttta tgggtgaaat
    18601 gcaacgacat tgtgctgttg cgctccacca gcagcgcgcg ttatttcgca ggtttcccga
    18661 ctggctatac cgcactgctc ggcgggttaa ccgagaacgg acgtagcgcg gtgaacgtgc
    18721 tttcgttcct ctgcctcgat gcctatcaaa gcgtacaatt accgcaaccg aacctagggg
    18781 tgcgcactaa cgccttgatc gacacgccgt tcctgatgaa aaccgcagaa accattcgcc
    18841 tcggcaccgg tattccgcaa atctttaacg atgaagtggt ggtgccagcg ttcctcaacc
    18901 gtggcgtttc gctggaagat gcgcgcgact attccgtagt gggctgtgtg gaattatcta
    18961 ttcccggcag aacctacggc ttgcatgaca tcgcgatgtt taatctgctg aaagtgatgg
    19021 aaatctgcct gcatgaaaat gaaggcaatg ctgcgctgac ttatgaaggt ttactggagc
    19081 agatccgcgc caagatcagc cactacatca ccctgatggt tgagggcagc aatatttgtg
    19141 atatcggcca tcgcgactgg gcacctgtac cgctgctctc gtcttttatc agcgattgtc
    19201 tggaaaaagg tcgcgatatt accgatggcg gcgcgcgtta taacttctcc ggcgtacagg
    19261 gaattggtat cgccaacctg agcgattctc tccatgcgtt gaaagggatg gtttttgatc
    19321 aacagcgttt aagttttgac gaattgctgt cggtattaaa agccaacttt gcaacgccag
    19381 aaggcgaaaa agtccgcgct cgcttaatta accgctttga gaaatacggt aacgatatcg
    19441 acgaggtggg taacattagc gccgaactgt tgcgccacta ctgcaaagaa gtggaaaaat
    19501 accagaaccc gcgcggcggt tacttcacgc cgggatcgta taccgtttct gctcacgtcc
    19561 cgttgggatc ggtggttggc gcgacgccag acggtcgttt tgccggagaa cagttggcag
    19621 acggcggctt gtcacccatg ctgggccagg acgcacaagg gccaacagcg gtactgaagt
    19681 cagtcagtaa gctcgataac acgctgctgt ctaacggtac gttgctgaac gtgaaattca
    19741 ctccggcgac cctggaaggt gaagcgggat tacgcaaact ggccgacttc ttacgggcgt
    19801 ttacccagct taagttgcag catattcagt ttaacgtggt gaacgccgac acgttgcggg
    19861 aagcgcaaca gcgcccacaa gattatgccg ggctggtggt gcgcgttgcc ggatacagcg
    19921 ccttctttgt cgaactgtcg aaggagatcc aggatgacat catccgccgg acagcgcatc
    19981 agctgtaacg ttgtggaaac gcgccgcaat gatgtggcgc gcattttcaa cattcagcgt
    20041 tattcactga atgacggcga gggcattcgt acggtggtct tttttaaagg ctgtccgcat
    20101 ctttgcccgt ggtgtgctaa tccggagtcg atctccggca aaatccagac ggtacgcaga
    20161 gaggcgaaat gtctgcactg tgcgaaatgt ttgcgtgatg cggatgaatg cccctccggg
    20221 gcgtttgaac ggattggtcg cgatatcagc cttgacgcgc tggaacggga agtgatgaaa
    20281 gatgacattt tctttcgcac gtccggcggc ggcgtcacgc tttctggcgg cgaagtgtta
    20341 atgcaggcgg agtttgctac ccgtttttta cagcggctgc ggttgtgggg cgtgtcatgc
    20401 gccattgaaa ctgccggaga cgcgccagcc agcaagctgt taccgctggc gaaattgtgc
    20461 gatgaagtgt tgttcgattt aaaaattatg gacgcgactc aggcgcggga tgtggtgaag
    20521 atgaacctgc cacgcgtgct ggagaatctg cgtttgctgg tgagtgaggg cgtcaacgtg
    20581 atcccgcgtt taccgctgat ccctggtttc acgctcagcc gggagaatat gcagcaggcg
    20641 ctggatgtac tgatcccgct gaaaatcagg cagatccatc tgttaccgtt tcatcagtac
    20701 ggcgaaccga aataccgcct gctggggaaa acatggtcga tgaaagaggt gcctgcgccg
    20761 tcgtcagccg atgtgacaac gatgcgcgaa atggcagaac gggccggatt tcaggttacc
    20821 gtgggaggtt aaaatggcat acctggtggc agtaaccgcc tgcgtaagcg gcgtggcgca
    20881 tacatatatg gcggcggaac ggctggaaaa gttgtgcctg ttagagaagt ggggagtcag
    20941 cattgaaact cagggcgcgc tgggaacgga gaatcgttta gcggacgagg atatccgtcg
    21001 ggcggatgtt gctctgttga ttacggatat cgagcttgcc ggtgccgagc gatttgaaca
    21061 ttgccgctat gtgcaatgca gcatctacgc attcctgcgt gagccgcagc aggtaatgag
    21121 cgcggtgcgc aaagtgcttt ctgcgccgca gcaaacccat cttattctgg agtagtcggt
    21181 ttttctgtca gttggctgtg gtactgccgg cgatattccg acggcgagcg ttcggtgttt
    21241 ttacgaaaca gacggcagaa gtagttgctg tcgacaaagc cgcaggcgtg cgccacttct
    21301 tttaccttca ggtcatagcc tttcaataac gtcttagcgt gctccagtcg cgtgtgattc
    21361 aggtattcgt taaaaccaat ggcccccgtt ttttgaaaca ggtgcgagag atagtttggt
    21421 gaaatataga atgcctgtgc gacagattcg cgggtaagcg cggaggcata gcgttcgtcg
    21481 atataatcgc gaatagcttc gaatagtgcc tggctgcgtg aggcggtctg gatttggctg
    21541 ccaagcaaat cgcggcagtg gctaagcagg ctggcgacga taagccttgc cgtttgctgc
    21601 tcctgcggct gcatttgcat ttcattgagc gtttgtagca gaaaagaacc tatgcgtggg
    21661 ccgcgtcggg cgacgtgttg cttcgccaga ttttgatatt gtttgccatc ccattgcacg
    21721 acgctgaagc cgagttgctg tttgccaaac agcacgctaa aggtagtagc aggggcttgc
    21781 cattgtggaa aattccagcc accagcggca acgtacagca catcgccggg aactaacgtt
    21841 gctccagcgc cggtatcaac aaactcacct tccagcacaa tttccagccg tggaaaatcg
    21901 acctgatacg caagatcggg caccgggccg ttactgctgg caaagtaaat ctggcgcagg
    21961 gacagcgggc cgttgatcag gcgggagagc agatagctga cgtcgtgata catgttattc
    22021 ccttaggtat ggagacggga aaacagtaac tggcattatt aaaaaaggcc acctgaaaag
    22081 tgtggcctga ggggagttcg atgtaaacat tgcggcagtt gtattgccgg atgtggcgta
    22141 aacgccttat ccagtctacc gccgcgatct gtaggccgga taagacgcgt caagcgtcgc
    22201 atccggcaaa tagcgcctgg ctgataatta ctgattacct acctggtcgc catacggcag
    22261 cgtgtcgtaa tcgatcagcg cgtttttctt gtacgggtta ccaatccagc gggtattttc
    22321 tttgaactgc ggattcacca ctgaacgggt tggatcgtaa ccgtcgtaag ataagcccgc
    22381 taaatctgac caggtgtgga tcagttccgc aaggctgtat ttacgatcaa catcctgcga
    22441 gaaatcacgc ggatgagtcg cttgccattt ttccgacgtc cacagcagga acggaatggt
    22501 gtacatatga cgcgtcgggt tgtcctcatt acgcccctgg gttttatgag gcggcgtgtc
    22561 gtaaacctct tcaccgtggt cagagaagta aaccaggaaa ccgttcgggt ctgctgcttt
    22621 aaagtcttta atcaggctgg caaccacatg atcgttatac aggttagcgt tgtcataatc
    22681 gttatatgac tccagctctt ccgcgtttaa tcccggcgga acatgatcgg tattgccatc
    22741 aaacttgccc tgattttccg ggtagcggta tttgtatttg atatgcgtac ccagcagatg
    22801 aacgatgatc agtttcttcg gcgcagggtc cttcagcact tcctggaacg gcttcagcac
    22861 gttagtgtcg tattcacgcg cactctgcgt acgttgctgg ttcatgtagt actgcttgtc
    22921 ggtctggcgc gaaaataccg tcagcatggt attgcgggcg gtcatcgtct gctggttggt
    22981 gatccagaag gttttataac ccgcctgttt catcatgttc atcagcgacg gctgcgtcag
    23041 atacagatcc gggttctttt cattggcaaa ggtcagcgcc tgttgcagga tttcaatggt
    23101 gtacggacga gacgtaacta cgttattaaa cacggtcaga ttcggatcgg ttttatgcag
    23161 cgcattcagc tccggcgtgg tttcacgcgg ataaccgtac agactcatgc gtccacgctg
    23221 ggttgactcg ccaatcacca gcactaaggt gcgcggttcg ttacccgatt catctttgaa
    23281 attagccagc ggcggcaagg cattattttc attcagtagc tttgttagcg agtttagttg
    23341 ctgacgatac tgataatagc cggtcaggaa ttgccacggt gcggcaggct ccatacgcga
    23401 ggccaggtta tccaacgttt tctcaaacgg cttgtttttg ataaacatat tcatggcgat
    23461 cggatgcaga atcaagccat aaagcagggc aaaagagaca acataacgcc acggctttgg
    23521 aatatagacc gggcgcaggc gtgtccacag cagaactgcc accgccgtat aggccagcgc
    23581 gataagcaca attttcaggc tgaaatactg gcttaaatac tcgctggctt cgttggtgtt
    23641 ggtttcgaac atcacaaaca gaacgctctg cgagaactcc tgaccgtaga tgacgtagta
    23701 gcacagcgcc gccagagagg ccgcccatag caccacgccg atcactgcgg caataatttt
    23761 gatccgcttc ggaaagagga ataccggaat taaccacagc gagctgaata acagcgagtc
    23821 gcgaatgccg ttggtgccac tataaccact gatgtaaata atggcctgta gcagagtaga
    23881 gaaaaaccaa aagtagagca atgcccaacc cagggctttc cagctaaaaa gaggtttagc
    23941 ctggacttct gtggaatgca tagtaagaac ctgtcttgaa aaaaatatcg ccgaatttac
    24001 cgacaattcc ttaaggatat ctgaagggat atccagaatt tgaataaaat gcagacagaa
    24061 atatattgaa aacgagggtg ttagaacaga agtatttcag aaaaccctcg cgcaaaagca
    24121 cgagggtttg caggagagga agattagccg gtattacgca tacctgccgc aatcccggca
    24181 atagtgacca ttaacgcctg ctcgacgcga ggatccggtt cctggccttc tttttctgcc
    24241 tggcgggagc ggtgcagcaa ctcggcttgc aatacgttca gcgggtcggt gtaaatattc
    24301 cgtagctgaa tagactctgc aatccacggc agatcggcca tcagatggga atcgttggca
    24361 atcgccagca ccactttgat gtcttcttct tgcagattgc gtaactcttt acctaacggc
    24421 cacagtgttt tgtctaccag gcgttggtca tagtattccg ccagccacag gtctgctttg
    24481 gcgaagacca tctccagcat gccgagacgc gtcgagaaga atggccaatc gcggcacata
    24541 gcctccagct cgctctgttt gccatcttcg accacttttt gcagcgccgt acctgcaccc
    24601 agccaggcgg ggagcatcag acggttttgc gtccaggcga agatccacgg aatggcgcgt
    24661 agtgactcga cgccgccggt tgggcgacgt ttcgccggac gtgaacccaa cggcagtttg
    24721 cccagttcct gttccggcgt agcggagcgg aagtaaggca caaaatcttt gttttcacgt
    24781 acgtagccac ggtagagatc gcaggagatg actgatagtt catccataat gcgacgccag
    24841 ctctctttcg gctcaggtgg tggcagcagg ttggcttcca gtatcgcccc ggtgtaaagc
    24901 gacaggctgc tgacggtgat ttctggcaga ccatatttaa agcggatcat ctcgccctgt
    24961 tcagtcacgc gcaggccacc tttcaggctt cctggcggtt gtgacagcag cgccgcatga
    25021 gcaggtgcgc cgccgcgacc aatggaaccg ccgcgaccgt ggaacaacgt cagctcaata
    25081 cccgcttttt cgcaggtttt gattaatgca tcctgtgcct gatattgcgc ccaggacgct
    25141 gccatcacgc ccgcatcttt tgctgagtcg gaatagccaa tcatcaccat ctgtttgccc
    25201 tgaatcaggc cgcgatacca gtcgatattc agcaactgag tcatgacatc gttggcgttg
    25261 ttcaggtcat cgagagtttc aaacagcgga gcaaccggca tcgcaaaccc gatacccgct
    25321 tctttcagca gcaggtggac agccagcacg tcggacggcg ttttcgccat cgagatcacg
    25381 taggcggcaa tggagccttg cggtgcttcg gcaatcacct ggcaggtatc gagcacttcg
    25441 cgcgtttcgg cgcttggttg ccagttgcgc ggcagaagcg gacgtttgga gttcagttcg
    25501 cggatcagga acgcctgttt gtcggcctct gaccagcttt cgtagtcgcc gataccgagg
    25561 tagcgggtca gctcgcccag cgcttcggta tgacgcgtgc tctcctgacg gatatcaata
    25621 cggaccagcg gtacgccgaa acatttcacg cggcgcaggg tgtcgagcag atcgccgttg
    25681 gcgataatac ccatgccaca cgcctgaagt gactggtagc aagcgtagag cggttcccac
    25741 agttcttcgt tttgtgtcag caggccttct ggttttggca gttcttcgcc tttcaggcgc
    25801 gcttccagcc atgcctgtgt cgccatcagg cgagaacgca ggtttttcat cagatagcga
    25861 tacggttctg cggcaccttc ttcgccaacc agcgccagca gttcaggggt cgcttcaacc
    25921 atcgacagtt cagaaaccag aacctgaata tctttcagga acagatcggt ggctttccag
    25981 cggctgagca gcaggacgtg gcgggtgata tcggcagtga cgttcgggtt gccgtcgcgg
    26041 tcaccgccca tccacgaggt aaaacgtacc ggaacaaatt cgacgggcag tttgtagccg
    26101 aggttctctt ccagttgttc gttcagttcg cgcaggtaat tcggtacgcc ttgccacagg
    26161 ctgttttcta ctacggcaaa gccccatttg gcttcatcta ccgggcttgg acgcagctta
    26221 cggatttcat cggtatgcca tgactgggcg atcaactggc gcaggcgacg catcagctgg
    26281 ttgtgttcgt agtcagcgat atctttgtta tcgagctgtt ttaagcaggc gttcacttcc
    26341 accattttgt ggatcagtgt acgacgggta atttcggttg ggtgagccgt gaggaccagt
    26401 tccagcgaca gcgattccac tgcttttttg atggtgtctt cgctcagttc cggctggttt
    26461 ttcagtttac gcagggtgcg ggcgatcact tccgggttgc tggcagcttc gcctttcggc
    26521 gaaatgctgt ggtattgctc ggcggtgttg gccaggttca ggaactgact aaacgcacgc
    26581 gcaacgggca gcagctcgtc gttcgacaaa ttttgtaagg tggtgagtaa ctcctggcgg
    26641 ttagcatcat tgccagcgcg tgaagatttg gacaacttac ggatagtttc tacgcgttca
    26701 agaatgtgtt ctcccaacgc atccttgatg gtttctccca gcactttgcc gagcatactg
    26761 acattactac gcaatgcgga atattgttcg ttcatattac cccagacacc ccatcttatc
    26821 gtttgatagc cctgtatcct tcacgtcgca ttggcgcgaa tatgctcggg ctttgctttt
    26881 cgtcgtcttt tataaagcca cgtaaaagcg gtgacgtcaa atgctgcgaa atcgcttcag
    26941 caaacgaata aatagcagga atttacgtca ttaaattcac gacgctttaa ataagcgtaa
    27001 cttatgggaa tgttaaaaaa tcgcctcaag taacaccaaa ggtgtaggtc ggataagatg
    27061 tgcaagcatc gcatccgaca ttattgcggc actggagttt ggcaacagtg ccggatgcgg
    27121 cgcaagcgcc ttattcggcc tacgttttaa tgccagcaaa aatggtgaat gacctgggtt
    27181 atcagttcgc gggtgggctt gataaaccgt gtttccagat attcatcagg ctgatgagcc
    27241 tgattaattg agccaggccc cagaaccagc gtcgggcata acgtttgaat aaacggcgct
    27301 tcggtacagt aattcaccac ttcggttttt gctccgagca atttctcaac cacttcaacc
    27361 agttgatgat tcggtgggca ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc
    27421 agacgacccg gccagcgttc gctcaccgga gccaatgcat cgttgagcaa accattaagt
    27481 tcattgagtg tcatgccagg cagcggacga atatccatat gcaactcaca ccaagcgcaa
    27541 atacggttag aagcgtcgcc accgtgaata tgcccgaggt tgagcgtagg gtatggcacg
    27601 gtaaacgctt cgtagtgata acgttctttc aggttatcgc gcaattgcaa aatatgcccg
    27661 atggcgtcgt gcattagttc tatagcgtta actccgcgtg ctggatcgct ggagtgcccc
    27721 gactggccct gaatacggat ggcgttagag atatgacctt tatgtgcgcg taccggttgt
    27781 agtgacgtcg gttcgccaat gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa
    27841 taacgcgctc cggccatact ggtttcttca tcagcagtcg ccagaatgta gagcggtttt
    27901 ttcagtttcg tgacgtcgac atcgcgtagc gcatcaagga taaacgcaaa aaagcctttc
    27961 atgtcggcgg tgcctaagcc gtaaagcttg ccgtcatgct ccgtcagtgt aaacggatcg
    28021 cgcgtccagc gaccgtcatc aaatggcacc gtatcggtat gccccgccag caacaagccg
    28081 ccagccccct gtccggtact tgccagcata ttaaatttgt tgcgagttcc tggtacaggc
    28141 tgcacttcca cattgaagcc taaatcttta aaccagtccg ccagcagagt gattaaatct
    28201 gcattgcttt gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc aatcagagca
    28261 cggtaaatct cgataaatgg cggtaatttg tttttcattg ttgacacacc tctggtcatg
    28321 atagtatcaa tattcatgca gtatttatga ataaaaatac actaacgttg agcgtaataa
    28381 aacccaccag ccgtaaggtg aatgttttac gtttaacctg gcaaccagac ataagaaggt
    28441 gaatagcccc gatgttgaat acgctgattg tgggtgccag cggctacgct ggcgcagagc
    28501 tagtgacctg tgtaaatcgc catccgcata tgaacataac cgctttgact gtttcagcgc
    28561 aaagcaatga tgcgggaaag ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg
    28621 atctgccgtt gcagccgatg tcggatatca gcgagtttag ccctggggtg gacgtggtgt
    28681 ttctcgccac cgcccatgaa gttagccacg atttagcgcc gcagtttctt gacgcgggct
    28741 gcgtggtgtt cgacctttcc ggcgcgtttc gtgttaacga cgccaccttc tatgaaaaat
    28801 attacggctt tacccatcaa tacccggaac tgctggaaca ggcagcctac ggtctggcgg
    28861 agtggtgcgg taataaatta aaagaagcga atttgattgc ggtgccaggc tgttatccga
    28921 cggcagcaca gttggcgctg aaaccgttga ttgatgccga tcttcttgac ctcaatcagt
    28981 ggccggtgat caacgccacc agcggcgtga gcggtgcagg acgtaaggca gcgatttcaa
    29041 atagtttttg tgaagttagc ctgcaaccgt atggcgtctt tacccatcgc catcaaccag
    29101 agatcgccac acacctcggt gctgacgtta tcttcacccc gcatctgggc aatttcccgc
    29161 gcggcattct cgaaaccatt acctgccgcc tgaaaccggg tgtgacccag gcgcaagtgg
    29221 cgcaagcgtt acagcaggcg tatgcccata aaccgctggt gcggctgtat gacaaaggcg
    29281 ttccggcgct gaaaaatgtc gttgggctac cattttgcga tatcgggttt gccgttcagg
    29341 gcgaacatct gattattgtg gcgaccgaag acaacttact gaaaggcgcg gcggcacaag
    29401 cggtacagtg cgccaatatt cgtttcggct atgcggaaac gcagtctctt atttaagggt
    29461 gcaatgatga atccattaat tatcaaactg ggtggcgtac tgctggatag tgaagaggcg
    29521 ctggaacgtc tgtttagcgc actggtgaat tatcgtgagt cacatcagcg tccgctggtg
    29581 attgtgcacg gcggcggttg cgtggtggat gagctgatga aagggctgaa tctgccggtg
    29641 aaaaagaaaa atggcctgcg ggtgacgcct gctgatcaga ttgacattat caccggagca
    29701 ctggcgggaa cggcaaataa aaccctgttg gcatgggcga agaaacatca gattgcggcc
    29761 gtaggtttgt ttctcggtga cggcgacagc gtcaaagtga cccagcttga tgaagagtta
    29821 ggtcatgttg gactggcgca gccaggttcg cctaagctta tcaactcctt gctggagaac
    29881 ggttatctgc cggtggtcag ctccattggc gtaacagacg aagggcaact gatgaacgtc
    29941 aatgccgacc aggcggcaac ggcgctggcg gcaacgctgg gcgcggatct gattttgctc
    30001 tccgacgtca gtggcattct cgacggcaaa gggcaacgca ttgccgaaat gaccgccgcg
    30061 aaagcagaac aactgattga gcagggcatt attactgacg gcatgatagt gaaagtgaac
    30121 gcggcgctgg atgctgcgcg cacgttgggc cgtccggtag atatcgcctc ctggcgtcat
    30181 gcggagcagc ttccggcact gtttaacggt atgccgatgg gtacgcggat tttagcttaa
    30241 gttttgttgg ccggaggcgc agctttccgg cattgaattt caaaataagg aaacagagtt
    30301 atggcacttt ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac
    30361 gactcactgc gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg
    30421 tccaaagccc tggtcacggt cggcgtgtta accgcagaag agcaggcgca actggaagag
    30481 gcgctgaacg tgctgctgga agatgttcgc gccaggccac aacaaatcct tgaaagcgac
    30541 gccgaagata tccatagctg ggtggaaggc aaactgatcg acaaagtggg ccagttaggc
    30601 aaaaagctgc ataccgggcg tagtcgtaat gatcaggtag cgactgacct gaaactgtgg
    30661 tgcaaagata ccgttagtga gttactgacg gctaaccggc agctgcaatc ggcgctggtg
    30721 gaaaccgcac aaaacaatca ggacgcggtc atgccaggtt acactcacct gcaacgcgcc
    30781 cagccggtga cgttcgcgca ctggtgcctg gcctatgttg agatgctggc gcgtgatgaa
    30841 agccgtttgc aggatgcgct taagcgtctg gatgtcagcc cgttaggttg tggcgcgctg
    30901 gcgggaacgg cttatgaaat cgaccgtgaa cagttagcgg gctggctggg atttgcttca
    30961 gcaacccgta acagtctcga cagcgtttct gaccgtgacc acgtgctgga actgctttct
    31021 gctgccgcta tcggcatggt gcatctgtcg cgttttgctg aagatctgat tttctttaac
    31081 accggcgaag cggggtttgt ggagctttct gaccgcgtga cttccggttc atcattaatg
    31141 ccgcagaaga aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag
    31201 ggcgcgttaa ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat
    31261 atgcaggaag acaaagaagg tctgttcgac gcgctcgata cctggttgga ctgcctgcat
    31321 atggcggcgc tggtgctgga cggcattcag gtgaaacgcc cgcgttgcca ggaagcggcg
    31381 caacagggtt acgcgaacgc cactgaactg gcggattatc tggtggcgaa aggtgtgccg
    31441 ttccgcgagg cacaccatat tgtgggtgaa gctgtggtgg aagccattcg tcagggcaaa
    31501 ccgctggagg agctgccgct caccgagttg cagaaattta gtccggtgat tggtgaagat
    31561 gtctatccga ttctgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa aggcggcgtc
    31621 tcaccacagc aggtggcgca ggcgattgct tttgcaaggg cgcggttggg gtaagaacat
    31681 ttatatgtac aaatttgagc ctggcttatc gccgggcttt tttatggcaa aaaaaagcgg
    31741 atcctggaga tccgcaaaag ttcacgttgg ctttagttat tcgagttgag aaactctcga
    31801 aacgggcagt gacttcaagg gttaaaagag gtgccgctcc gtttctgtga gcaattatca
    31861 gtcagaatgc ttgataggga taatcgttca ttgctattct acctatcgcc atgaactatc
    31921 gtggcgatgg aggatggata atgaatattc gtgatcttga gtacctggtg gcattggctg
    31981 aacaccgcca ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg
    32041 ggcaaattcg taagctggaa gatgagctgg gcgtgatgtt gctggagcgg accagccgta
    32101 aagtgttgtt cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg
    32161 aggtgaaagt ccttaaagag atggcaagcc agcagggcga gacgatgtcc ggaccgctgc
    32221 atattggttt gattcccacg gttggaccgt acctgctacc gcatattatc ccgatgctgc
    32281 accagacctt tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg
    32341 cgcaactgga cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag
    32401 cattcattga agtgccgttg tttgatgagc caatgttgct ggctatctat gaagaccacc
    32461 cgtgggcgaa ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc
    32521 tggaagatgg tcactgtttg cgcgatcagg caatgggttt ttgctttgaa gccggggcgg
    32581 atgaagatac acacttccgc gcgaccagcc tggagacgct gcgcaacatg gtggcggcag
    32641 gtagcgggat cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg
    32701 ttgtttatct gccgtgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc
    32761 ctggctcacc gctgcgcagc cgctatgagc agctggcaga ggccatccgc gcaagaatgg
    32821 atggccattt cgataaagtg ttaaaacagg cggtttaaac cgtttaacgc agctacccga
    32881 taggcttccg ccatcgtcgg gtagttaaag gtggtgttga cgaagtactc aatagtgttg
    32941 ccgccacctt tctgttccat aatcgcctga ccgatatgaa taatttcggc agcgcgctcg
    33001 ccaaagcagt gaatgcccag aatctctttt gtttcccgat ggaacaaaat tttcagcgtg
    33061 cccacgttca tgccgacgat ttgtgcacgt gccagatgtt tgaactgggc gcggcccact
    33121 tcatatggca ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga gctgatttcc
    33181 gggatggtgt aaatgccggt agggatatct tcaatcagat gcgcggtggc ttcgcctttt
    33241 accagcgcct gcgcggcaat gcgcccctgg tcataggccg ctgatgccag gctcggataa
    33301 ccaatcacgt cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat gctgttgacc
    33361 ttcagctgtc cgcgactgtc agtttccagc ccaatgttct gtaacgccag cgaatcggta
    33421 ttaccggtac gaccgttggc atagagcagg cagtcagctt tcagtttttt acccgacttc
    33481 agatgcatga tcacaccgtc gtcacagcct tcgatcttct cgtactcttc gttgtgacga
    33541 atcactacgc cactgttcca gaagtgatag gagagagaat ctgacatctc ttgatcgaga
    33601 aatgccagca ggcgatcgcg ggtgttgatc agatccactt ttacatccat accgcggaag
    33661 atcgacgcat attcacagcc gatcactcca gcaccataga taagtacatg gcgcggttcg
    33721 tggtgcatgc tgagaattga gtcgctgtcg taaatgcgtg gatgggtgaa atcaacatct
    33781 gttggatgat atggacgaga gccgcaggca ataacaaatt tttcagcggt tagtgtttca
    33841 acgctgccgt ccgggcaatc cagcgccaac gtatgctcgt caacaaagcg agcgttcccc
    33901 tgcaatattt cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta
    33961 atcacgttat cggcatggtt aaggatatcg gcaaaagaag agcggagcag tcgggaatgg
    34021 tcgctgtata gtgggttttg attgaattct ataatgcggc tgacggcgtg acggagagct
    34081 ttcgacggga tggtgcccca gtgggtgcaa ccgccgccaa cattttgata acgctcgata
    34141 actgcgacgc gcgcaccttg cttaaccagg cccattgcag cgccttcgcc gccggggccg
    34201 gaacctatta ctatggcatc gtaatcgtag gaatgtggca tggtagggct tacctgttct
    34261 tatacataaa agcaacagaa tggtaacatt ttatcgcggg taagccaatt gatccccgtc
    34321 attaatctgg ctatagcgtg agcggccttt gctttgtctg cctccttctt aacccattta
    34381 ttcactatta atcgcttttt attatggctg atagttttat cttgccgaag cgtgcttttt
    34441 tgttattgtc tttgtgaaat cttcttataa aacaaacata taaatatttt atattgatga
    34501 gttatttgta tttacaccat caataaaatc cgttaattag cctgtttata agtgaataaa
    34561 ggaatagccg atgatcgaac actatattcg agggtttgag gaagaactgc gtgagatccg
    34621 ccatcagatc cacgaaaacc cggagctggg attacaagaa tttaaaacca gtgctttggt
    34681 tgcggaaaag ctgcgtcagt ggggctatga agtagagcaa ggactggcga cgacggggat
    34741 agtggccacg ttaaaagtgg gcgacggaga gaaaagtatt ggcctgcggg cggatatgga
    34801 tgcgctgccg atttatgaaa atagtggcaa gccctgggcc agtaaacatc ctggtttaat
    34861 gcacgcctgc gggcatgacg ggcataccac catactgctg ggggccgcgc gttattttgc
    34921 cgaaacccgc cgttttaacg gcacgttacg tcttatcttc cagcctgcgg aagagatgat
    34981 taatggcggt gagatcatgg ttaaagaggg gctttttgat catttcccct gcgatgtcat
    35041 tttcggcatg cataacatgc ccggtctgcc ggtgggtaag ttttatttcc agccaggggc
    35101 actgatggcg tcaatggatc agttccatat tacagttcgt ggttgtggcg gacacggtgc
    35161 gatcccgcac aaagccatcg atccggtgct agttgccgca catatcacca ccgcattaca
    35221 aagcattgtg tcacgcaatg tcgatccgct ggaagcggcg gtaattaccg tcggcagcat
    35281 tgttgcgggt gaggcggcta acgtcattcc tgacagtgct gagatgaaaa tcagcgtccg
    35341 ctctcttagc cgcgataccc gacaacttct tctgacccgt attcctgctc tggcacaagc
    35401 ccaggctgcc agctttggcg ctacggctga ggttacgcat gttaacggta caccagtatt
    35461 agtaaatgac gaggagatgg cgcgcttcgc ctggcaggtg gcgtgtaaaa cgtttggtga
    35521 agatcgggcc gagtttggca tcaagcccct gatgggcagt gaagatttct ctttcatgct
    35581 ggaagcccaa cccaaaggcg gtttcctgtt attcggtaat ggcgatgtcg gggaaggttc
    35641 ctgcatggtg cataacccgg gttacgactt caacgatgca agtctggtcc cggcaagcag
    35701 ctactggggc gcactggtag aagcctggct gcaataaata aaacaacaca caacaacact
    35761 ttttggcaag gaacatggaa tgacggcgat cgtaaatgaa accgtgagtg tgaagaaaaa
    35821 gcccagcggc aggcgggtga tttttgcctc ggcgtttggc aatgcgctcg aattttttga
    35881 ttttggcgtt tacaactttt ttgtggtcta tatcagcacg ctattttttc cgcccagtgc
    35941 agataaaaac gtcgcgctac tgctggcgtt tgccactttc ggcgtgagct tttttatgcg
    36001 cccgctcggc ggcattattg tgggggcctg ggccgatcgt ttcggacgca aacccgcgat
    36061 ggtgttcacc attgcgctga tgagtcttgg tacactgatg atcggcatcg caccgacgta
    36121 tgaaacggcg ggctattggg gaaccgcgac gctggtgcta gcgcgtctaa ttcagggcgt
    36181 tgcggcagga ggcgaagtcg gtgcctcaat gtctttgttg gttgaatcag ctccggcaaa
    36241 tcgtcgcggt ttttacagca gttggtcact ggcgactcaa ggcctggcaa caacttttgg
    36301 aggtgttgtc gctttaggtt taagtgcatg gctgcccttt gcgaccggtt cagaaaccgt
    36361 catggctgaa tggggctggc gtgtgccgtt ttttattggc gtgttattag cgcctgtcgg
    36421 ctgttggttg cgtttgagtc tggaaaatga tgttcctgag ccagcacata ataagaaggc
    36481 cgcagccagc gaaagcgcct tctccttact tatgcaacat aaagcgacta tcgctaacgg
    36541 tatcttgctc gctattggta gtacagtcgc aacctatatc tccctctttt attacggtac
    36601 atgggcggcg aagtatttag gaatgaacca aaactattct catgcagcga tgttactggc
    36661 gggcgttatt acgtttgtgg gtgcgctgct ggtggggatg ttgtgcgatt cggtcgggcg
    36721 taaaaagctg attttaatct cccgtgtgat ggtgctgatc tgtagctggc cgtcattctg
    36781 gctgttggtg aattacccaa gcccaggcat gttgctgacg gtggttttcg tgatggtcag
    36841 ctttaccacg cttggcggtg tgccagtgat gttgttgatc tctgaactgt tgccgaaacg
    36901 gattcgggca ttgggttttg cgctggttta tagcattggt gtggcaatct ttggcggttt
    36961 tgctcaatat ttcgctacgc aatccatcgt gttgctggat agtttgacgg ctccggcgtg
    37021 gtatctgggt ggaggcacgt tgctgtcgat gttggcgttg ttgtacgtaa aagaaccggc
    37081 caaagaattg cagtaaatat gttttattgc gttaccgttc attcacaata ctggagcaat
    37141 ccagtatgtt cattctctgg tatagtgcca gcagtacttt tggcaaggat tcagacatcg
    37201 tgatgggcgt aagagcgcaa caaaaagaaa aaacccgccg ttcgctggtg gaagccgcat
    37261 ttagccaatt aagtgctgaa cgcagcttcg ccagcctgag tttgcgtgaa gtggcgcgtg
    37321 aagcgggcat tgctcccacc tctttttatc ggcatttccg agacgtagac gaactgggtc
    37381 tgaccatggt tgatgagagc ggtttaatgc tacgccaact catgcgccag gcgcgtcagc
    37441 gtatcgccaa aggcgggagt gtgatccgca cctcggtctc cacatttatg gagttcatcg
    37501 gtaataatcc taacgccttc cggttattat tgcgggaacg ctccggcacc tccgctgcgt
    37561 ttcgtgccgc cgttgcgcgt gaaattcagc acttcattgc ggaacttgcg gactatctgg
    37621 aactcgaaaa ccatatgccg cgtgcgttta ctgaagcgca agccgaagca atggtgacaa
    37681 ttgtcttcag tgcgggtgcc gaggcgttgg acgtcggcgt tgaacaacgt cggcaattag
    37741 aagagcgact ggtactgcaa ctgcgaatga tttcgaaagg ggcttattac tggtatcgcc
    37801 gtgaacaaga gaaaaccgca attattccgg gaaatgtgaa ggacgagtaa tgaaacaagc
    37861 aaatcaagat agaggtacgc tgctgctggc gttggttgct ggcttatcga ttaatggtac
    37921 tttcgcagcg ctgtttagct ccattgtgcc attttctgta ttcccgatta tttccctggt
    37981 gctgacggtt tactgcctgc atcaacgtta tcttaatcgc accatgccgg taggcttgcc
    38041 gggtctggca gctgcctgtt ttattctcgg cgtactgctg tacagcacgg tagttcgtgc
    38101 ggaatatccg gatatcggct ctaacttctt cccggcagta ctctccgtca ttatggtgtt
    38161 ctggattggc gcgaagatgc gtaaccgtaa gcaggaagtt gctgagtaat cggtatcatg
    38221 ccggatgctg gcgcatccgg catgggtttt acttcgcggt cagtaatacg ccgcactcca
    38281 tatggtgcgt gtaggggaac tgatcaaaca gagccagacg ttcgaccttg tgcgtctggc
    38341 ttaatgtttc cagatttttg cataacgttt ccggattgca ggagatatac agaatacgtg
    38401 gatacgcctg caccattttc tcggtttcac tgtccagacc gctgcgaggt gggtcgacaa
    38461 aaatggtttc gcactgataa ctctttaagt cgatcccttg caggcggtta aactcgcgca
    38521 cgccattcat cgcctgagta aattcttctg ccgccatacg aataatttgt acgttatcaa
    38581 tatggttagc ggcaatgttg tattgcgcgg cagcgactga tggcttggcg atttcggtgg
    38641 ctaatacccg atcaaaattg cgcgccagcg ctaatgagaa gttaccgttg ccgcagtaca
    38701 gctccagtaa atcgcctttt gagcctttgg ttacgtccag cgcccattcc agcatctgaa
    38761 tattcatcgc cgcgttcggc tgggtaaagc tgttttctac ctgacgatag atcatctctt
    38821 tccctgcgac cggcagacgc tcgtcgatgt aatcttgatc cagcgcgatt ttggttttcg
    38881 ttgcccgacc aatcagatgc acattcagat tctgcgcgcg cagtgcatcg cgcagggctt
    38941 ccgcttcctg acgccactca tcatccagct ttttatggta cagcagggaa accaccgcct
    39001 gattactcag cgtggtgagg taatcaatct ggaacaactt gtggcgcaga acgggattat
    39061 tacgcacacc cgcaatcatc gccgtcatca actggttgat gagttcactg gcggcgggga
    39121 agctatccac ccggatacgg cttttggttt gttgatcgaa aatgatgtgg tacaggtcat
    39181 cgccatcgtg ccagatgcgg aactccgcac gcatccggta atgactgacc ggcgagcgaa
    39241 acacttccgg aaccaggtca gaaaacggtg ccatcatact ttgcaaacgt accacttttt
    39301 cggctaactg cgcttcatac tgttctgttg gaaggtgttc gggggtcatg atgtatcctg
    39361 aaaaattaaa gtacgcggcg attgtaggga ttgctcatca gatgtccaga tcttgatgaa
    39421 ttcctatttg tgagctacgt ctggacagta acttgttaca acctgtagca tccacttgcc
    39481 ggtcctgtga gttaataggg aatccagtgc gaatctggag ctgacgcgca gcggtaagga
    39541 aaggtgcgat gattgcgtta tgcggacact gccattcggt gggaagtcat catctcttag
    39601 tatcttagat acccctccaa gcccgaagac ctgccggcca acgtcgcatc tggttctcat
    39661 catcgcgtaa tattgatgaa acctgcggca tccttcttct attgtggatg ctttacaatg
    39721 attaaaaaag cttcgctgct gacggcgtgt tctgtcacag ccttttccgc ttgggcacag
    39781 gataccagcc cggatactct cgtcgttact gctaaccgtt ttgaacagcc gcgcagcact
    39841 gtgcttgcac caaccaccgt tgtgacgcgt caggatatcg accgctggca gtcgacctcg
    39901 gttaatgatg tgctgcgccg tcttccgggc gtcgatatca cccaaaacgg cggttcaggt
    39961 cagctctcat ctatttttat tcgcggtaca aatgccagtc atgtgttggt gttaattgat
    40021 ggcgtacgcc tgaatctggc gggggggagt ggttctgccg accttagcca gttccctatt
    40081 gcgcttgtcc agcgtgttga atatatccgt gggccacgct ccgccgttta tggttccgat
    40141 gcaataggtg gggtggtgaa tatcatcacg acgcgcgatg aacccggaac ggaaatttca
    40201 gcagggtggg gaagcaatag ttatcaaaac tatgatgtct ctacacagca acaactgggg
    40261 gataagacac gagtaacgtt gttgggcgat tatgcccata ctcatggtta tgatgttgtt
    40321 gcctatggta ataccggaac gcaagcgcag ccagataacg atggtttttt aagtaaaacg
    40381 ctttatggcg cgctggagca taactttact gatgcctgga gcggctttgt gcgcggctat
    40441 ggctatgata accgtaccaa ttatgacgcg tattattctc cgggttcacc attggtcgat
    40501 acccgtaaac tctatagtca aagttgggac gccgggctgc gatataacgg cgaactgatt
    40561 aaatcacaac tcattaccag ctatagccat agcaaagatt acaactacga tccccattat
    40621 ggtcgttatg attcgtcggc gacgctcgat gagatgaagc aatacaccgt ccagtgggca
    40681 aacaacatca tcattggcca cggtaatgtt ggtgcgggtg ttgactggca gaagcagagc
    40741 acggcaccgg gcacagctta tgttaaggat ggatatgatc aacgtaatac cggcatctat
    40801 ctgaccgggc tgcaacaagt cggcgatttt acctttgaag gcgcagcacg cagcgacgat
    40861 aactcacagt ttggtcgtca tggaacctgg caaaccagcg ccggttggga attcatcgaa
    40921 ggttatcgct tcattgcttc ctacgggaca tcttataagg caccaaatct ggggcaactg
    40981 tatggcttct acggaaatcc gaatctggac ccggagaaaa gcaaacagtg ggaaggcgcg
    41041 tttgaaggct taaccgctgg ggtgaactgg cgtatttccg gatatcgtaa cgatgtcagt
    41101 gacttgatcg attatgatga tcacaccctg aaatattaca acgaagggaa agcgcggatt
    41161 aagggcgtcg aggcgaccgc caattttgat accggaccac tgacgcatac tgtgagttat
    41221 gattatgtcg atgcgcgcaa tgcaattacc gacacgccgt tgttacgccg tgctaaacag
    41281 caggtgaaat accagctcga ctggcagttg tatgacttcg actggggtat tacttatcag
    41341 tatttaggca ctcgctatga taaggattac tcatcttatc cttatcaaac cgttaaaatg
    41401 ggcggtgtga gcttgtggga tcttgcggtt gcgtatccgg tcacctctca cctgacagtt
    41461 cgtggtaaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact
    41521 gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct
    41581 ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc
    41641 ggatctccat tacatttatg ctttcgataa cgtcgctttc ccgtatggcg aaaaaagcga
    41701 agcgtttatt gttgagcgag tggtggcaat tgtcaccgcg gtgcaagaac gttatcccct
    41761 tgcgctggct gtggtcgctt gcaacactgc cagtaccgtt tcacttcctg cattacgcga
    41821 aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac
    41881 ggcaaatggc attgtcggat tactggcaac ccgcggaaca gttaaacgtt cttatactca
    41941 tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat
    42001 ggttgagttg gctgaagcga agctgcatgg cgaagatgtt tctctggatg cactaaaacg
    42061 tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac
    42121 ccatttccct ctactacaag aagaactgtt acaagtgctg ccagagggaa cccggctggt
    42181 ggattctggc gcagcgattg ctcgccgaac ggcctggttg ttagaacatg aagccccgga
    42241 tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag aagctgaaca
    42301 attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg
    42361 ctgatttggt tgaatgttgc gcggtcggaa aattatttta aatttcctct tgtcaggccg
    42421 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
    42481 cagcggggtt ctcctgagaa ttccgacaga gaaagcaaaa ataaatgctt gactctgtag
//
LOCUS       DAZFTN010000027        41969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFTN010000027 DAZFTN010000000
VERSION     DAZFTN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..594
                     /locus_tag="ACHKV2_001648"
     CDS             61..594
                     /locus_tag="ACHKV2_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6939550.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            644..1327
                     /locus_tag="ACHKV2_001649"
     CDS             644..1327
                     /locus_tag="ACHKV2_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6939551.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1340..3790
                     /locus_tag="ACHKV2_001650"
     CDS             1340..3790
                     /locus_tag="ACHKV2_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6939552.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3803..4763
                     /locus_tag="ACHKV2_001651"
                     /pseudo
     CDS             3803..4763
                     /locus_tag="ACHKV2_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(4801..5700)
                     /gene="nhaR"
                     /locus_tag="ACHKV2_001652"
     CDS             complement(4801..5700)
                     /gene="nhaR"
                     /locus_tag="ACHKV2_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO6939553.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5766..6932)
                     /gene="nhaA"
                     /locus_tag="ACHKV2_001653"
     CDS             complement(5766..6932)
                     /gene="nhaA"
                     /locus_tag="ACHKV2_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO6939554.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            7518..7670
                     /gene="mokC"
                     /locus_tag="ACHKV2_001654"
     CDS             7518..7670
                     /gene="mokC"
                     /locus_tag="ACHKV2_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO6939555.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(7774..8904)
                     /gene="dnaJ"
                     /locus_tag="ACHKV2_001655"
     CDS             complement(7774..8904)
                     /gene="dnaJ"
                     /locus_tag="ACHKV2_001655"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6939556.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8993..10909)
                     /gene="dnaK"
                     /locus_tag="ACHKV2_001656"
     CDS             complement(8993..10909)
                     /gene="dnaK"
                     /locus_tag="ACHKV2_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO6939557.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            11286..11690
                     /gene="yaaI"
                     /locus_tag="ACHKV2_001657"
     CDS             11286..11690
                     /gene="yaaI"
                     /locus_tag="ACHKV2_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO6939558.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11716..12429
                     /gene="msyB"
                     /locus_tag="ACHKV2_001658"
     CDS             11716..12429
                     /gene="msyB"
                     /locus_tag="ACHKV2_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6939559.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRPHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            12578..13144
                     /gene="satP"
                     /locus_tag="ACHKV2_001659"
     CDS             12578..13144
                     /gene="satP"
                     /locus_tag="ACHKV2_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO6939560.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(13179..13766)
                     /gene="mog"
                     /locus_tag="ACHKV2_001660"
     CDS             complement(13179..13766)
                     /gene="mog"
                     /locus_tag="ACHKV2_001660"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO6939561.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(13881..14834)
                     /gene="tal"
                     /locus_tag="ACHKV2_001661"
     CDS             complement(13881..14834)
                     /gene="tal"
                     /locus_tag="ACHKV2_001661"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6939562.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            15113..16543
                     /gene="yaaJ"
                     /locus_tag="ACHKV2_001662"
     CDS             15113..16543
                     /gene="yaaJ"
                     /locus_tag="ACHKV2_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO6939563.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            16613..17389
                     /gene="yaaA"
                     /locus_tag="ACHKV2_001663"
     CDS             16613..17389
                     /gene="yaaA"
                     /locus_tag="ACHKV2_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO6939564.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(17542..17838)
                     /gene="yaaX"
                     /locus_tag="ACHKV2_001664"
     CDS             complement(17542..17838)
                     /gene="yaaX"
                     /locus_tag="ACHKV2_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO6939565.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(18052..19338)
                     /gene="thrC"
                     /locus_tag="ACHKV2_001665"
     CDS             complement(18052..19338)
                     /gene="thrC"
                     /locus_tag="ACHKV2_001665"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO6939566.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(19339..20271)
                     /gene="thrB"
                     /locus_tag="ACHKV2_001666"
     CDS             complement(19339..20271)
                     /gene="thrB"
                     /locus_tag="ACHKV2_001666"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO6939567.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(20273..22735)
                     /gene="thrA"
                     /locus_tag="ACHKV2_001667"
     CDS             complement(20273..22735)
                     /gene="thrA"
                     /locus_tag="ACHKV2_001667"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO6939568.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(22761..22874)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(22816..22899)
                     /locus_tag="ACHKV2_001668"
     CDS             complement(22816..22899)
                     /locus_tag="ACHKV2_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO6939569.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(23113..23799)
                     /gene="yjtD"
                     /locus_tag="ACHKV2_001669"
     CDS             complement(23113..23799)
                     /gene="yjtD"
                     /locus_tag="ACHKV2_001669"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6939570.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(24199..24339)
                     /gene="yjjY"
                     /locus_tag="ACHKV2_001670"
     CDS             complement(24199..24339)
                     /gene="yjjY"
                     /locus_tag="ACHKV2_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO6939571.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            24435..25151
                     /gene="arcA"
                     /locus_tag="ACHKV2_001671"
     CDS             24435..25151
                     /gene="arcA"
                     /locus_tag="ACHKV2_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO6939572.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(25211..26563)
                     /gene="creD"
                     /locus_tag="ACHKV2_001672"
     CDS             complement(25211..26563)
                     /gene="creD"
                     /locus_tag="ACHKV2_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO6939573.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(26621..28045)
                     /gene="creC"
                     /locus_tag="ACHKV2_001673"
     CDS             complement(26621..28045)
                     /gene="creC"
                     /locus_tag="ACHKV2_001673"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO6939574.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(28045..28734)
                     /gene="creB"
                     /locus_tag="ACHKV2_001674"
     CDS             complement(28045..28734)
                     /gene="creB"
                     /locus_tag="ACHKV2_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO6939575.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(28747..29220)
                     /gene="creA"
                     /locus_tag="ACHKV2_001675"
     CDS             complement(28747..29220)
                     /gene="creA"
                     /locus_tag="ACHKV2_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO6939576.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            29431..30300
                     /gene="robA"
                     /locus_tag="ACHKV2_001676"
     CDS             29431..30300
                     /gene="robA"
                     /locus_tag="ACHKV2_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO6939577.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(30297..30944)
                     /gene="gpmB"
                     /locus_tag="ACHKV2_001677"
     CDS             complement(30297..30944)
                     /gene="gpmB"
                     /locus_tag="ACHKV2_001677"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO6939578.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            30996..31508
                     /gene="yjjX"
                     /locus_tag="ACHKV2_001678"
     CDS             30996..31508
                     /gene="yjjX"
                     /locus_tag="ACHKV2_001678"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO6939579.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(31655..31981)
                     /gene="trpR"
                     /locus_tag="ACHKV2_001679"
     CDS             complement(31655..31981)
                     /gene="trpR"
                     /locus_tag="ACHKV2_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO6939580.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(32071..34008)
                     /gene="sltY"
                     /locus_tag="ACHKV2_001680"
     CDS             complement(32071..34008)
                     /gene="sltY"
                     /locus_tag="ACHKV2_001680"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO6939581.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            34219..35886
                     /gene="ettA"
                     /locus_tag="ACHKV2_001681"
     CDS             34219..35886
                     /gene="ettA"
                     /locus_tag="ACHKV2_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO6939582.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(35993..36160)
                     /gene="nadS"
                     /locus_tag="ACHKV2_001682"
     CDS             complement(35993..36160)
                     /gene="nadS"
                     /locus_tag="ACHKV2_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6939583.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(36194..37426)
                     /gene="nadR"
                     /locus_tag="ACHKV2_001683"
     CDS             complement(36194..37426)
                     /gene="nadR"
                     /locus_tag="ACHKV2_001683"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO6939584.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(37447..38829)
                     /gene="radA"
                     /locus_tag="ACHKV2_001684"
     CDS             complement(37447..38829)
                     /gene="radA"
                     /locus_tag="ACHKV2_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO6939585.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(38878..39846)
                     /gene="serB"
                     /locus_tag="ACHKV2_001685"
     CDS             complement(38878..39846)
                     /gene="serB"
                     /locus_tag="ACHKV2_001685"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO6939586.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            39952..40593
                     /gene="ytjB"
                     /locus_tag="ACHKV2_001686"
                     /pseudo
     CDS             39952..40593
                     /gene="ytjB"
                     /locus_tag="ACHKV2_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            40624..41640
                     /gene="lplA"
                     /locus_tag="ACHKV2_001687"
     CDS             40624..41640
                     /gene="lplA"
                     /locus_tag="ACHKV2_001687"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO6939587.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            41672..41935
                     /locus_tag="ACHKV2_001688"
     CDS             41672..41935
                     /locus_tag="ACHKV2_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6939588.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT        10458 a        11012 c        10156 g        10343 t
ORIGIN      
        1 gatcacataa tcgggtttga aaacgagtga ccttccatac cagttaatta aggatgattt
       61 atgaaaaagt atattattcc cttcgttgcg acaacgttga tgtttagcgc aacaaccttc
      121 gccaaggatg tcgatcaagg aacactaacc attagaggag aagttgttga tacaacgtgt
      181 tactttgtta atggtgattc gacaacagat atcgttgtcg aagatgttgt aaccagtgca
      241 atgaatcaac ttatcaataa tgaaatctat actttaaaaa ccggttcaac ctctcatgac
      301 ttagaaattc agtgtgacgg aaatacagcc ccaagtatcg aagtactagc atcagagttt
      361 gatgctaata actttacgct gaataaagga actgctgaga acattggctt tgccattttc
      421 ctcgatgatg ggactttgac cagaatgaga ccagggttaa aaacaccgct gaaagataat
      481 ggctctggtc aatattttct caatttaaat gcacaatatg cccgcacaag tggcaatcct
      541 gttacaaaag gctccgtaga gtcaaccgta acgattaaaa tcagtgcaga ttaacgtttt
      601 ttgccggtag ctctgctacc ggtattattt acggagtgat tacgtgagaa taataacagc
      661 attactgatg tcatttttta ttttacctgt tcatgccggt gtggtcatct atggtacgcg
      721 cattatttat ccggaaaaaa acagagaagt tctcgtacag ttaatgaatc aaagtaaaaa
      781 tgcctctttg attcaggcat ggatcgatga tgggaacacc actatcgcgc cagaaaaaat
      841 tcaggtcaca tttattctca caccgccagt aagtcgtgta gcaggtggtt ctggccaaca
      901 acttaaaata agaaaaatgc caaacaattt gcctcacaat aaagaaagtt tattttatct
      961 aaatgttctg gatatccctc caaacaatcc acaaaatgca gggaaaaaca aaattaaact
     1021 tgcattgcaa aaccggataa agttactttg gcgtccaagc ggaatagctc cggtagataa
     1081 aaaaagcctt tctcaattaa atatcaaaaa gaaaaataac gccatctcaa taaacaacga
     1141 aacagccaac tggataacgg ttacaactat caaagcacaa aacgtgaaag ttaataatga
     1201 aagtatctta ctgcctccct tttcgcataa cgacattaca ttaaaaaata accacgcatc
     1261 agaatatgaa ctaacggtag ttgatgatta cgggaataac atccatagta aaatcgccgc
     1321 gagataacag gaaaaagtca tgttaaggac gacacgatgg gtcgctgcta ttattttttt
     1381 gtattctttt ccgggttatg ctgaagaaac ttttgatacg cactttatga tcggcggcat
     1441 gaaaggagaa aaagtttccg aatatcattt cgataataaa caaccgcttc caggaaacta
     1501 cgaacttgat ttttatgtaa ataaccagtg gcgtggaaaa caagatatca cgatcccaga
     1561 gtcaccagtc aaaccatgtc tgcctaaggt gctactaacc aaactggggg taaagaccgg
     1621 taacctcaat acagaagata attgtatttt actggataaa gcggttcatg gtggacaata
     1681 tcagtgggat atcagtgaac accgactaaa tttaacagta ccacaggcgt atatcaatga
     1741 acttgaaaga ggttacgttc ccccggaaag ttgggatcgc ggcattgatg ctttttatac
     1801 ctcctataat ttaagtcagt atcgctctta tgacagcaat aataacagta atacagccag
     1861 ttatggccga tttaacagtg gattaaattt atttagttgg cagttacact ctgatgccag
     1921 ctatagcaaa cccgatgata tgaaaggcac atggcaaagt aatactcttt acctggaaca
     1981 tggctggtcg cagatattaa gcacagttca gataggagaa aattacacat cctcactcat
     2041 attcgactct ctgagattta gcggaatacg cttatttcgc gatatgcaaa tgctacctga
     2101 ttctatgcag agctttacac ctttagtaca aggtgttgcg caaagtaatg ccttgataac
     2161 ggtttcgcaa aatggctaca tcatttacca aaaagaggtt cccccgggac catttactat
     2221 cgcagatttg caactctcgg gcagcggctc tgatctcgat gtcagcataa aggaagcaga
     2281 tggcagtgtt cgttcctttt tggtgcccta ctcttccgtg cctaacatgc tgcaacctgg
     2341 tatttcaaat ttcgatttta tcgccggacg cagtaagata tatggtgtaa agaatcagga
     2401 ggactttctc gaggcaaatt atatatatgg cctcaacaat cttttgacac tttatggcgg
     2461 tacgatttta tccgataact ataatgctat aactctggga aatggttgga atacaccgct
     2521 gggtgccatc tcttttgacg ctacacgatc gagtagtaag ttaaataatg atataacaca
     2581 cgaaggtacg agctatcagg tcgcctataa taaatatcta gtacaaacgg ccactcgatt
     2641 tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg accatcttta
     2701 cgaaaatgat aaaataaatc atcagagtga ctatgacgat ttttatgata ttggcagaaa
     2761 aaatagcctt tctgccaata tcatgcaacc tttatccaat aatctgggaa atgtatcatt
     2821 aagtgcgctt tggcggaatt actgggggcg aagtggaaat gctaaagatt accaattcag
     2881 ttattccaat agctggcaac gcatcagtta tactttctct gccagccaat cctatgatga
     2941 aaacgacaaa gaagaggagc gttttaatct gtttatctcc attcctttct actgggggga
     3001 tgatattgcc aaaacacgtc accaaattaa cttatcgaat tcgacctcat tttccaaaga
     3061 tggctattcc tccaacaata ctggaattac tggcatagcc ggtgaacatg atcagttaaa
     3121 ttatggtata tatgttaatc agcaacaaca aaataatgat acatcgcttg gtacgaattt
     3181 aagctggaga actcccatcg ccacaataga tggcagctat agccattcta aaaacgcctg
     3241 gcaaagtggt ggaagtatta gtagtggatt agttgtctgg cccggtggta ttaatatcac
     3301 taaccagcta tccgatacat ttgcaattct ggatgcgcct gggttagaag gcgcgcatat
     3361 taatggacaa aaatacaacc gaacaaacag caaaggccag gttgtttacg acctgatgat
     3421 acctcatcgt gaaaaccatc tggtacttga tacagcaaac agtgaaagtg aaacagaatt
     3481 gcagggcaat cgtcaaatta ttgcgcctta ccgtggagca gtttcttatg tgcagtttac
     3541 aactgaccaa cgtaagccct ggtatataca ggcactgcgt cccgatggtt cgccattaac
     3601 ttttggctat gacgtactgg atctccagga aaacaatatt ggagtcgttg gccagggtag
     3661 tcgccttttt attcgcgtag atgaaattcc aactggcata aaagttgctc tcaatgatga
     3721 acagaattta ttctgtacta ttacttttca acacgttatc gatgaaaaca aaacatatat
     3781 atgccagtaa aaggagttta ctatgaagtg gcttttatta atcacattga gtctatattc
     3841 ttttattgtg caaagtgctc cctgtggatt gaccaatgtc ggggaacaac gtggaacata
     3901 tatactcaaa ccactgagta tgaaaggtaa tctcaccgcc gaaaaaattg aaaatactgg
     3961 gaatatcatt gagtgtgcag acttgcaaga gacgaccaac gccattaaag ttcatttttt
     4021 aacacagaac gctttaaggt ttaaagtcgg aagtcgtcat tatattatta atttcccctt
     4081 tgaatctgac ggtaaaaaaa atagcgcttc cagggaagtc atcttataat ttagaagaaa
     4141 tgctatctgc gataaatttc accattccat actctatcgc atctgtatca gatagttcca
     4201 acaccgcaga tatttttcca ggaactcctt ttcctttgtt gttcgaaatt gaagtgagtt
     4261 catgtacagg gaataatact aattgtaata gcgtccgctt taattttaat ttaaatacta
     4321 ttttacaggt aaatttaatg acctgtggtt ttgaagatca atccatcgac actggacgat
     4381 ttgtactgtc tcgtatcaat aatgaaaatt atcagtttca tcatataaga tttgactgca
     4441 tccagggcga gcaaggtgaa ttagtttttg aacccagtga cgttgaatat tactttgaac
     4501 cagtgacgat acaggagtca ggaaccagca tattaaaaaa tgagttagaa aacagtgagg
     4561 acggtgcggg tcaaataggc tttcaactct ccaatgatgg tacgaatgaa atccagtatg
     4621 gtaaaagcaa ttactactct tttcagcatc cccatgaagg aagtaatcag attcctctat
     4681 ttattcgccc gcgtacatat ggaaacaatg tctcaagcgg gcaaataatg agccgagtca
     4741 aaatcgtggt aatgtacaac taatctatct cccccacctt aactttgggg gagctgccga
     4801 ttaacgcacc gctggactaa aaagcgcaga ataatccgta ttgcagattc gctgtaccgc
     4861 cgggtgctga atcatccgct cagcaaaaat agcatggtac tcttccatca cattctcgac
     4921 gcgaccaatt tctacgacag ttttatcggc ataaaagtca tatgcataaa gcgttggggc
     4981 aacgaagatt gcattgtgca tcgcaccaaa agctttcatc aaagcggcat catcaaactc
     5041 gccaaggatt tctacgttta atccctggga gttaaaccag ttaagtaatt tgcgccctaa
     5101 cattgaacgt cgcccaggaa tcaaaagccg ccgttcttca agacaagctg ggaacggttt
     5161 ttccggcggt ggatttgtac accagaaact cacgccacat tcgccaattc tcacggagaa
     5221 caggccttcc tgctgcgtag aatctatcgg acagtcggaa atgatcatat ccagtttatg
     5281 ctggcttaat tgctccagca gcatttcgtg ggtggattcg aagcagcgaa gatgaatcgg
     5341 ctcgccttct actactgcag cattcagcac gctactgacc agacgtttgg aaagtacatc
     5401 agccacgcca acgtcaaaca ataaattgga ttctttgcga tagttcacga tatccagcat
     5461 ttcctggctc aaggtaaaca ttttatcggc atagcgatag accagctccc ccagctcgct
     5521 gggctcgaga ccacgtccct tgcgcttaaa taatttgcct tgcaggcgct cttccagcgc
     5581 ccgaatctgt ccggtaatgg tttgtggtgt taaataaagc gcctccgctg cgccaaccac
     5641 agaaccttct ttataaacat gccagaagta atacaagtgg ttgtaattga tatgagacat
     5701 gctcatttct ctccctgata acaatgaaaa gggagccgtt tatggctccc cggtaaaccg
     5761 tcctgtcaaa ctgatggacg caaacgaacg cgtaaccagc tgtatccaat taccgccgaa
     5821 gagatagaac cgaccaggat accgagtttc gcccagttaa tcagttctgg atctacgcta
     5881 ccaaaggcca ggctggcaat aaagatagac atagtaaaac cgataccgca caggatcccc
     5941 accgccataa tttgctgata agtcgttccc tcaggcagat gcgccagttt caaacgcagc
     6001 gccaaccagc agaacagact aatccccagc ggtttgccaa tcagcaagcc agcgatgatc
     6061 cccaatggca gaatggaggt caagccatcc agcgtgacgc cttgcagtga aacgccagca
     6121 ttagcaaatg caaacagtgg taaaatcaga tacgccaccc acgggtgcaa cacatgctcc
     6181 agtcgcttcg ctggagaacg cccatgcttc tctttcaaag gaataaagaa gccgacaatt
     6241 acccccgcca gggttgcgtg aacccccgat ttcaacactg cagtccacaa cactacgcca
     6301 accagaatat agacgcccgt gcggcgtaca ccacacagat tcaataccgc gagtacagca
     6361 attgctacag ccgcgacgcc aagagaggcc atcgataagt cattagtgta gaacaatgcg
     6421 atgataatga tggccccaag atcgtcgata atagccagag ccatcaaaaa gatcttcagc
     6481 gctaacggaa cacgacttcc caatagcgcc agcacaccaa gtgcaaaggc aatgtcagta
     6541 gccgccggga tcgcccaccc ttcgcgggta atcggatcgg catagttaaa ggccagatag
     6601 agtaatgccg gcacaatcat cccaccaata gcagcgataa ctggaaatgc ggcctggcgt
     6661 aagctggcta gcgatccttg catcagttca cgtttaactt ccagaccgac taacaggaaa
     6721 aataccgcca tcagcgcgtc atttatccat aacagcatgt ttttgttgat ttcgagtgaa
     6781 ccaacccgga gctgaaccgg cgtctccaga aagtcgtgat accatccact ggttgcgccg
     6841 ctgttggcca tcatcatcgc caggatagcg gcaatgataa gaataatgcc tcccgaggca
     6901 tcactgctaa agaatcgatg cagatgtttc actttttatt tctctttcag gtgaatagat
     6961 cgtttttact attttacccc gcacgaatca tcgttaaaat cagattatag tgtaggaata
     7021 attcgttttt tacgatctat agaagaattt gcgccatcag cggtgagatg cgtttttgac
     7081 ggctgtaagc gctgacgggt gaccctaagc cctgataatc atagcttaag gtgaggattt
     7141 ctgagggatt aattaggaaa agtaataatt aatttgttgt cagtcggcga tgtttgttag
     7201 gccaatattt caaaaataat ccagcttgtc gtcaataatt gcgatgaaga gataaattaa
     7261 agttaaataa ttaaatcatt aaaaaattac gccataaaaa tgcgaggcgt cttccggatt
     7321 agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg tgcttgaggc
     7381 tgtctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg atattttacc
     7441 atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact gaccgtaagg
     7501 tcaaggagaa gagagcaatg aagcagcata aggtgatgat tgtcgccctg atcgtcatct
     7561 gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag gttcacatcc
     7621 gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa gagcaacggc
     7681 ggggagtgat ccccgccact ttacaggtgc tcgcatatct tcaacgcacc ctatttttac
     7741 ccaggcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt caaaaaactt
     7801 cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcgccgg ttgggccacc
     7861 gaagctttct tgcagctctt gcagcagctg tttctgcttc tcgttcaggc ctaccggtgt
     7921 ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag acttgacgcc
     7981 tttaccgcgc atacggaata gcttaccggt ctgggtttcg ccaggcactt tcagtttgac
     8041 gcgaccatca agggtcggta cttcgatttc accacccagc gccgccatag cgaagttgat
     8101 cgggacttcg caatacaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg
     8161 aacctgaacg tacagatcgc ctgccggtgc gccgtgttca cccgcttcac cttcgcccgc
     8221 aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct
     8281 gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg
     8341 gccctgacag tgtggacagg tctgctgcac ggcaaagaaa ccctggcgca tctgcacctg
     8401 accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg caccgctacc
     8461 gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca cgccacgtac
     8521 agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg ccgcacgttg
     8581 acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat cgctgaagtc
     8641 tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag catgaccata
     8701 ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt ccttgatctc
     8761 tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt atttcatggc
     8821 caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa cgcctaaaat
     8881 ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat gcacgggcgg
     8941 agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc gattattttt
     9001 tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg ttgtttgcag
     9061 aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt tccatcagtt
     9121 tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg tcttcacctt
     9181 tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg tcagccggca
     9241 gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg tcgccctggt
     9301 tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct tcggcgtcgc
     9361 gtaccatttt ctggatttca tcttcgttca gaccagaaga cgccttgatg gtgatcttct
     9421 gctctttacc gctgttttta tctttcgcgg aaacgtgcag gataccgtca gcatcgatat
     9481 cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca tccaggttga
     9541 actgacccag agatttgttg tcagccgcac gtttacgttc accctgcagc acatggatgg
     9601 ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg gtcgggatag
     9661 tggtgttttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata cccagagaca
     9721 gcggggtaac atccagcagc agtacgtctt ttacgtcacc agtcagaaca ccaccctgaa
     9781 cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc tctttaccaa
     9841 agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccacca acgaggataa
     9901 cgtcgtcgat atcagatacg gacaggccag cgtcctgcag cgcaactttc agcggctcga
     9961 tggaacggtt aaccagatct tcaaccaggc tttccagttt cgcacgagtc actttgatgt
    10021 tcatgtgttt cggaccggtc gcgtctgcag tgatgtacgg caggttaacg tcggtctgct
    10081 gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc tgcattgcca
    10141 gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc agatagttga
    10201 tcagacggct gtcgaagtct tcaccaccca ggtgggtatc accgttggtt gccagaactt
    10261 cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg aaagtaccac
    10321 cacccaagtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga ccgtaagcca
    10381 gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg atacggcctg
    10441 cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg ataacagctt
    10501 cagttaccgg ttcacccagg taatcttcag cggttttctt catttttttc agcacttcag
    10561 cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg tcgccgttat
    10621 cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct tcgtcctgga
    10681 agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc gtcactgcct
    10741 gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca atgatagaag
    10801 gcgtggtgcg atcgccttcg gcgttctcca gcacacgagg agtggtgcca tccataatcg
    10861 ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc taaacgtctc
    10921 cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa cgtctggttt
    10981 caactgccca atttcatttt tttgcagact cactcactgc ggttgactac taaatggggt
    11041 cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc gcagaaattt
    11101 cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata aagaatgaat
    11161 cgcagtcaag gtattcatca aaaaattgta ataaaaagaa aagattacgt gcctgaatct
    11221 tctctttatc agcagtaaac tagtgggtat tcatccccct acctcttccc actaagagaa
    11281 tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg atgctgtgct
    11341 gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg cgtaacgaaa
    11401 ccaacgctct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata caactctctg
    11461 ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc gcccgtagcg
    11521 ccagtcagag cctgaatatt ccttcagaaa taaaagaagg gcaaaccact gactggatca
    11581 acattaacag cgataacgac aataaacgct gcgtctcaaa aatcaccttt tccggtcata
    11641 cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa cgccagaata
    11701 tagaagccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga ttttctccag
    11761 cattgtagtg aggaacagtt agcaaatttc gcccggctgc tcacccataa tgaaaaaggc
    11821 aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat ggaaggacat
    11881 cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca ttttggtggc
    11941 gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat tttgctcgat
    12001 gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga aattgagcaa
    12061 cagttactgg aacaatttct gcgtaatacc tggaagaaaa tggacgagga acataagcag
    12121 gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct gccgctgctg
    12181 atgaaagaca aattattggc aaaaggcgtg tcgcatctgc tttccagcca actgacccgc
    12241 attttacgcc cccacgcagc aatgagcgta cttgggcatg gtttgctgcg cggcgcgggg
    12301 ctgggaggcc ctgtaggcgc ggcactaaat ggggttaaag cggtcagcgg cagctcctat
    12361 cgcgtgacga ttccagccgt actgcaaatc gcctgcctgc gccggatggt tagcgccact
    12421 caggtctgat accggaaacc ggggaaaatt ttcattttcc cctcaaaaga tcgtagacac
    12481 tgccccactg gctgattatt atgccgcgcc ctgaaaacac tacagttatt cagggaaatt
    12541 atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta agttggctaa
    12601 tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta acctgcacaa
    12661 cgtaggttat ttcgctctgg acggtattat tcttgccatg ggcattttct acggcggcat
    12721 cgcgcaaatt tttgctggtc tgctggagta caaaaaaggc aacactttcg gtttaaccgc
    12781 attcacctct tacggttctt tctggctgac gctggttgcg attctgctga tgccgaaact
    12841 gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc tgtggggcgt
    12901 atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc aattcgtttt
    12961 ctttagcctg accgtgctgt ttgccctgct ggcgatcggt aacattgccg gtaacgccgc
    13021 aatcatccac tttgccggct ggattggtct gatctgcggt gccagcgcaa tctatctggc
    13081 gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg gtgaatccca
    13141 ctaattgtct tgagatcccc ccgctcgggg ggattttttt attcgctaac gtcgcgtctt
    13201 gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta tggcccttcc
    13261 agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac gttaccctca
    13321 gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg taagttaagg
    13381 atcagcgcct gtttgcgaat cacacccacc tgacgcgaaa ggatcgcagt tggtacaaaa
    13441 tgcaggctga tctggcgcat ctgttcacca aagccaggca tctcgcggtc cgctactgcc
    13501 agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc
    13561 agatggcaac tcatttcatc caccagctca cacaacgttt gctcgatgat cgcctgctca
    13621 tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt cagccattct
    13681 tccagcgcag ggataccttt atcctgataa acgccgctgg atgcgcgatc agagatggaa
    13741 actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc tcgttaaaca
    13801 gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg tgaccgactt
    13861 cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag tttttcctgg
    13921 tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg atcctggttg
    13981 tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac ttcgccggtg
    14041 taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag cagtgccggt
    14101 gcgatggtca gacggtcgca gcctgccagt tccagaattt cgccgatgtt acggaagctt
    14161 gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat ttcagataca
    14221 gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcggtatt cgctttgtac
    14281 cagtcaagaa tacggccaac aaacggcgag atcaggaaca cgcccgcttc cgcacaagca
    14341 cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgataccttc tttttccagc
    14401 tgttccgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat acgatcgttg
    14461 ctaatacctg catcgttgta gagtttgatc aggcgttttg ctttcgcaat tgacgcttct
    14521 gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac cagtttcagg
    14581 atttccagac caatatttac tgccagtttg tcagtcgcgt ccacgatctg ctgcgcgcga
    14641 tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg gtattccgga
    14701 atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg ttgatacagc
    14761 ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg aagggaggtc
    14821 aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat gtaaccggtc
    14881 tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag
    14941 gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt taaacaaaac
    15001 ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt cttaaacaat
    15061 tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat gcatgccaga
    15121 ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc tgctcttcgg
    15181 cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt
    15241 tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct catttcagtc
    15301 attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc
    15361 tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt ttatcggcat
    15421 ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg acgtcaatgg
    15481 gcagtttcgt ggcggaccgg catggtatat ggcgcgcggg ctggggatgc gctggatggg
    15541 cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg gagttcaggc
    15601 aaacgctgtt gcccgagctc tgagtttttc ttttgatttt cccccgctgg tgacaggcat
    15661 tattcttgct gtctttgctc tgctggcaat cattcgcggt cttcatggcg tcgcccggct
    15721 catgcagggg tttgtcccgt tgatggcgat aatctgggta ctgaccagcc tggtgatttg
    15781 cgtaataaat atcgggcaac ttccccacgt catttggtct atttttgaga gtgcttttgg
    15841 ctggcaggaa gcggcaggcg gcgcggcggg atatacctta agccaggcga ttactaacgg
    15901 ttttcagcgc agtatgtttt ccaatgaggc gggaatgggg tcgacgccaa acgcggcagc
    15961 ggcagcggcg tcctggcctc cgcatccggc agcgcaagga attgtccaga tgattggcat
    16021 ttttatcgac accctggtca tctgtacggc aagcgccatg ctgatattac tggcgggtaa
    16081 cggcacaacc tacatgccgc tggaaggtat tcagcttatc cagaaggcga tgcgggtgct
    16141 aatgggttcc tggggtgctg aatttgttac cctcgtggtt attctgtttg ccttcagctc
    16201 catcgttgcc aactacattt atgccgaaaa caatctcttc tttttacgcc tgaacaaccc
    16261 taaagcgatc tggtgtttgc ggatctgcac cttcgcaacg gtcatcggcg gcaccttgct
    16321 aagtcttccg ctgatgtggc aactggcaga tatcataatg gcctgcatgg ctattaccaa
    16381 tttgactgcc atattactgc tctcgcctgt ggttcatacc attgccagtg attatctacg
    16441 ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg atatcggtcg
    16501 ccagctttct cgggacgcgt gggatgatgt ttcgcaggag taatcacaac tatcgatcaa
    16561 ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg atatgctgat
    16621 tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca cgcgatatac
    16681 actgccggag ctgttagaca attcccagca gttgatccat gaagcgcgga aactgacgcc
    16741 tccgcagatt agcacgctga tgcgtatcag cgacaaactg gcaggtatca acgccgctcg
    16801 ctttcatgac tggcagccag atttcacgcc ggagaatgcc cgccaggcga ttctggcgtt
    16861 taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg atttcgattt
    16921 tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactccgcc cgctcgattt
    16981 aatgcagcct tatcgtctgg aaatggggat ccgtcttgag aatgcccgag ggaaagatct
    17041 gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg cagcacaagg
    17101 cgataatgtg gtgattaatc tggcgtcaga tgagtatttt aaatcggtga agccgaagaa
    17161 attgaatgcc gagattatca agccggtgtt cctcgatgag aagaacggca aatttaagat
    17221 aatcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta ttgaaaatcg
    17281 gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct ttgatgaagc
    17341 ttcctccagc aatggcgaac tggtgtttaa acgctacgag cagcgttaag tttgtagtca
    17401 atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcatgcagat tcaatatatt
    17461 gcagaattca cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct gaatgccgga
    17521 ttgttacccg gcatttgtca tttagcggtg atgtttgcct ggaccatgac cgccgtgatg
    17581 atcatgatga gctttcttat ggtgacgcgg cggtggcggc ggtccgtgtg ggtgccagcg
    17641 attgcctcgc cattcataat gttgtttcca ccagccgtgg tcgcgccagt gaccgccatc
    17701 ccagtaataa ccgcgattat cacgatcgcc tatctgtaat tttactgacg ggactaacgt
    17761 aatttccgca gcctgcgttg ccatgggagc gaccagaacc agggaaagtg cgagtacgat
    17821 agattgcatc tttttcacag gttactcctt ttatcattgc ccgttatggg tcgatatccg
    17881 gagattacgc gatgagagcg gcggttgtta ttctccgcaa tcctaatctc taagttaccg
    17941 catttattga gaatttctcc tttttccctg tcgtttctct ccatattttc ttcacaaaaa
    18001 agccgggctg cataaaagca aacccggcct gattgagata atgaatagat gttactgatg
    18061 attcatcatc aatttacgca acgcagcaaa atcggcgggc agattatgcg aaagcaaggg
    18121 taaatcggca cgttctgcca gctcttttgg cagatccaac gtttcaccga gaatcgcttc
    18181 cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat attcgcctgg
    18241 attcaactgg tcacgcagcg cacgatacgc tacggcagcg tgcggctccg aagtgtagcc
    18301 cagttctttt aactcacgca ttgtctgttg cgtggtttca tcatccacgg cggcataacc
    18361 cagctctttc agttgccaga ttttgcggcg gaacaactct tccacacgcg gccagttgtt
    18421 cggctgactc acgtccatcg cgttggataa cgtcgcctga gtcgctttgg gtgaccactg
    18481 accgtcatgc aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa taaaacgttt
    18541 caccggcaga ccgagtgact tcgccagcag acccgccgtc aaatcgccga agtttccgct
    18601 tggcaccgag acaaccagct ggttgcgcgc ttcctgcggc agctgcgcaa ctgcttcaaa
    18661 gtagtagcaa atctgcgcca gcaaccggct aatgttaatc gagttagctg agtttaaccc
    18721 cagcgccact ttcagctctt catcatcaaa cgcctgcttc accagcgcct gacaggcatc
    18781 gaaatcgccg tcgatggcaa cagtttcgat attgccgccc aatgtacaga acagtttttc
    18841 ttgcagtgga ctgattttgc ctcgtggata aaggataacc actttcacat tcggtaaacc
    18901 gtagaaagca tgagccactg ccgctccggt atcaccggag gtcgcggtca gaatggtcac
    18961 tggcttatcg cccgcaatat gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt
    19021 aaatgccagc gttggcccgt ggaacaattc cagacaaccg acatcgcttt caacattggc
    19081 gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct tccaggattt cctgcgggat
    19141 ttcatcacca ataaacgccg agaggatctt cgcactgcgg gtgacaaaat ccagcttcag
    19201 catctcatca atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa acagcccctg
    19261 atttttgccc aacccctggg ttacggcttg cgcaaagctg acctgctcat tgtgatcttt
    19321 aagattgtag agtttcattt agttttccag tactcgtgcg cccgccgtat ccagccggca
    19381 aatatgaaca aaaccttcct gattttgcag gtagttctta cccaaccagt cggcaacgcg
    19441 ctgggcggta tccggcttgt cacacagagc aaacaaggtc gggccggagc cggagatacc
    19501 gctcgctacc gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc caggcagtaa
    19561 ccgttcacgg tagggttctg cgataacatc tttcatcagc ttcgcggcaa gctcaggctg
    19621 acgggaatag caggcgtgaa tgaagcctgc caagtgtcgc ccgtgcgcaa tgcaatcctg
    19681 gcggcgatac tgcgccggta aaatagcccg ggcttctgcc gtcgagactt taatccccgg
    19741 atacgccagc acccacagcc actcatcaaa ccctggcact tgctggctga tgatgtcgtt
    19801 ttcttcgatc atcaactgca taccaccaag aaaacacggt gccacgttgt cgtaatgaat
    19861 gctgccggag attcgtcctt ccagctcgcc catcaaagcc agcaaacggg tgtcattaag
    19921 cggcttgccg cagtgttcat tcatcgccat cagagccgcg accaccgaac aggcgctgga
    19981 gcctaagccc gaaccgatcg gcatattctt ttccagagtc atcgccactg gaatttgctt
    20041 gcccagctcc tggcaaaaac gctcccagca ctgataaacg atattttccc gtggttctga
    20101 cggcagctta tcggcaaagc gtccgaggtt gttgagactg aatgtctctg ccgactcaac
    20161 cgtgactaca tctccgagca atgcaccatc aacgggtgtc accgccgccc cgagcacatc
    20221 aaacccgacg ctcatattgg cactggaagc cggggcataa actttaacca tgtcagactc
    20281 ctaacttcca tgagagggta cgtagcagat cggcaaagac accggcagcg gtaacgtcat
    20341 tgcccgcacc atatccgcgc agcaccaacg gcagcggctg ataatagtgg ctataaaagg
    20401 ccagggcgtt ttcgccattt ttcactttga acagcggatc attaccatcc acttcggcaa
    20461 tcttcacgcg gcagacgcca tcttcatcaa tattgccaac atagcgcaaa acttttcctt
    20521 catcacgggc cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac agattcgcca
    20581 taaaagcggc aacatcaccc tcagcgttaa actctgcggg cagcacaggt tcaatttcaa
    20641 tatccgccag ctccagttca cgtcccgttt cacgagcgag aattaatagt ttacgcgcta
    20701 catccatacc agaaagatca tctcgcggat ccggttcggt ataacccatt tcccgcgcca
    20761 gcgtagtcgc ctcggagaaa ctcatgcctt cgtctaactt gccgaagata taagaaagcg
    20821 aacctgaaag aatgccggag aacttcatca attcatcacc agcattgagc agattttgca
    20881 ggttctcaat aaccggtaat ccagccccaa cgttggtgtc atagaggaat ttacgccgcg
    20941 atttttcagc cgcatgacgc aacagatggt agtaatccat cgacgaggtg ttggcctttt
    21001 tgttcggcgt gacaacgtgg aaaccttcgc gcaggaagtc ggcatattga tccgccactg
    21061 cctggctgga ggtgcagtca acaatgaccg ggttcagcag atgatattct ttcacgaggc
    21121 gaattaagcg cccgagatta aacggctctt tggcttgcgc cagttcttcc tgccagtttt
    21181 ccagatttag gccatgcaca ttggtgagca gagccttcga gttagcaaca ccgcagacac
    21241 gtaagtcgat atgtttattc ttcaaccagc tttgctgacg cttcagttgc tccagcagcg
    21301 caccgccaac gccaccgacg ccaatcacaa acacttcgat aacctgatcg gtattgaaca
    21361 gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc gttatttacc acgacagaga
    21421 ttgagcgttc agaagatccc tgagcaatag cgacaatgtt gatattggcg cgggccagcg
    21481 cggcaaagaa tttcgccgag atcccacgca aggtgcgcat accatcacct accaccgaga
    21541 taatggccag ccgttccgtc accgccagcg gctccagtaa gccttctttc agttccaggt
    21601 agaactcttc ctgcattgcc cgttcagctc gcacacagtc gctttgcgga acgcagaaac
    21661 tgatactgta ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca
    21721 ttgcagcaaa gacgcgcgcc gccatgccga ccattccttt catccccggg ccggaaacgc
    21781 tgaacattgc catattattc agattggaaa tgcccttgac cggtaattcg tcttcatcac
    21841 gactggcacc aatgagcgta cctggagctt gaggatttcc ggtattttta atcaggcaag
    21901 ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta gcgccgaagt
    21961 aggaaagctc catcgcttcc tggtaggaca tcgatttcaa caacctcgca tcgggcacct
    22021 gacgcgggtc gcaggtatat accccgtcaa cgtccgtcca aatctcgcaa caatcggcgc
    22081 gtaaacaggc agccagcacc gccgcggagt agtcggaacc gttgcgtcca agtaccacca
    22141 gttcgccttt ttcattaccg gcggtgaaac ctgccatcag caccatgtga tcagccggaa
    22201 tacgacttgc cgcaatacgg cgggtggact ctgcaatatc gacagtagat tcgaggtaat
    22261 gccccactgc cagcagtttt tcgaccggat cgataacggt aacgttgtga ccgcgcgctt
    22321 ctaatacgcc ggccataatg gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt
    22381 taatgctatc cgggcactgc cctaacaaac taatgccatg caggacatgt tttatttggg
    22441 caaattcctg gtcgacgaaa gttttcaatt gcgccagcgg gaatcccggc tgggcggcgg
    22501 cgagtcccgt cagaagttcg gcaaaaatac gttcggcatc gctgatattg ggtaaagcat
    22561 cctggccgct aatggttttt tcaatcatcg ccaccaggtg gttggtgatt ttggcggggg
    22621 cagagaggac ggtggccacc tgcccctgcc tggcattgct ttccagaata tcggcaaccc
    22681 gcagaaaacg ttctgcattt gccactgatg taccgccgaa cttcaacact cgcatggttg
    22741 ttacctcgtt acctttggtc gaaaaaaaag cccgcactgt caggtgcggg cttttttctg
    22801 tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtaatgg tgatggtggt
    22861 ggtgatggtg gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt actctgtaat
    22921 ttttatctgt ctgtgcgcta tgcctatatt ggttaaagta tttagtgacc taagtcaata
    22981 aaattttaat ttactcacgg caggtaacca gttcagaagc tgctgtcaga gactcttttt
    23041 ttaatccaca cagagacata ttgcccgttg cagtcagaat gaaaagctga aaaatactta
    23101 ctaaggcgtt ttttatttgg tgatattttt ttcaatatca tgcagcaaac ggtgcaacat
    23161 tgccgtgtct cgttgctcta aaagccccag gcgttgttgt aaccagtcga ccagttttat
    23221 gtcatctgcc actgccagag tcgtcagcaa tgccatggct cgttcgcgta aagcttgcag
    23281 ttgatgttgg tctgccgttg tgtcactttt cgtcggttgt tgtattaata ttgctaattg
    23341 atagcaatag accatcaccg cctgccccag attgagcgaa ggataatccg ccaccatcgg
    23401 cacaccagta agaacgtcag ccaacgctaa ctcttcgtta gtcagcccgg aatcttcgcg
    23461 accaaacacc agcgcggcat ggctcatcca tgaagatttt tcctctaaca gcggcaccag
    23521 ttctactggc gtagcgtagt aatgatattt cgcccgactg cgcgcagtgg tggcgacagt
    23581 gaaatcgaca tcgtgtaacg attcagccag tgtcgggaaa actttaatat tatcaataat
    23641 atcaccagat ccatgtgcga cccagcgggt ggctggctcc aggtgtgcct gactatcgac
    23701 aatccgcagt tcgctaaacc ccatcgtttt cattgcccgc gccgctgccc caatattttc
    23761 tgctctggcg ggtgcgacca gaataatcgt tatacgcata ttgccactct tcttgaccaa
    23821 atgaccgcga acagggtgat cactgtcaac ttattacgcg gtgcgaattt acaaattctt
    23881 aacgtaagtc gcagaaaaag ccctttactt agcttaaaaa aggctaaact atttcctgac
    23941 tgtactaacg gtttaattgt taaaaaatgc tacatatcct tctgtttact taggataatt
    24001 ttataaaaaa taaatctcga caattggatt caccacgttt attagttgta tgatgcaact
    24061 agttggatta ttaaaataat gtgacgaaag ctagcattta gatacgatga tttcatcaaa
    24121 ctgttaacgt gctacaattg aacttgatat atgtcaacga agcgtagttt tattgggtgt
    24181 ccggcccctc ttagcctgtt atgttgctgt taaaatggtt aggatgacag ccgtttttga
    24241 cactgtcggg tcctgaggga aagtacccac gaccaagcta atgatgttgt tgacgttgat
    24301 ggaaagtgca tcaagaacgc aattacgtac tttagtcatg ttacgccgat catgttaatt
    24361 tgcagcatgc atcaggcagg tcagggactt ttgtacttcc tgtttcgatt tagttggcaa
    24421 tttaggtagc aaacatgcag accccgcaca ttcttatcgt tgaagacgag ttggtaacac
    24481 gcaacacgtt gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa gcgacagatg
    24541 gcgcggaaat gcatcagatc ctctctgaat atgacatcaa cctggtgatc atggatatca
    24601 atctgccggg taagaacggt cttctgttag cgcgtgaact gcgcgagcag gcgaatgttg
    24661 cgttgatgtt cctgactggc cgtgacaacg aagtcgataa aattctcggc ctcgaaatcg
    24721 gtgcagatga ctacatcacc aaaccgttca acccgcgtga actgacgatt cgtgcacgca
    24781 acctgctgtc ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt agcgttgaaa
    24841 gctacaagtt caatggttgg gaactggaca tcaacagccg ttctttgatc ggccctgatg
    24901 gcgagcagta caagctgccg cgcagtgagt tccgcgccat gcttcacttc tgtgaaaacc
    24961 caggcaaaat tcagtcccgt gctgaactgc tgaagaaaat gaccggccgt gagctgaaac
    25021 cgcacgaccg tactgtagac gtgacgatcc gccgtattcg taaacatttc gaatctacgc
    25081 cggatacgcc ggaaatcatc gccaccattc acggtgaagg ttatcgcttc tgcggtgatc
    25141 tggaagatta atcggcttta ccaccgtcaa aaaaaacggc gctttttagc gccgttttta
    25201 tttttcaacc ttatttccag atacgtaatt catcgtccgt tgtaacttct ttactggctt
    25261 tcattttcgg cagtgaaaac gcataccagt cgatattacg agtcacaaac atcatgccgg
    25321 ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca gagttgagga
    25381 gtccccacat cacaccatcc agcaacaaca gcgcgagggt aaacaacatg ctgttgcgcc
    25441 aacctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc agactggcga
    25501 ttatccatgc cacggtaaaa ccggtatgtt cagaaagcgc cagcaagagc aaataaaaca
    25561 tcaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc gcggtgagcg
    25621 tttcaaaaac aaagaacgcc ataaaagtca gcgcaatcag cagaatggca tacttagtcg
    25681 cccggtcagt taattggtat tgatcggcgg gcgtcgtcac tgcgacgcta aacgcgggga
    25741 agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta ttagcaaacc
    25801 agctgctttg ccattgcgcc tgaaaacctg actcactaac ttcccgtttg gctggtagaa
    25861 aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg ctattacgcc
    25921 ccgcaggcac cacagaaaga tcgccggtac cgcttaaatt cagtgccata ttcagcttca
    25981 ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc ccgccttgct
    26041 ctaacccgat gccgggttca atagtcagcg ccgttccgtt aacttcaggc gctttcacca
    26101 caccaatacc acgcgcatcc ccgacgctaa tcacaataaa tggcttgcca aaggtgatat
    26161 ttggcgcgtc gagttcgcta agacgcgaaa catcgaaatc ggcttttagc gttaaatcac
    26221 tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc tgattgccat
    26281 caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc tccacggttt
    26341 tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataaga ggcccaacga
    26401 gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta cggtaatcgg
    26461 cacgttcgac aatcacctgc cgaatcagca ttatcggaat caacaacagc aatactgcac
    26521 caaacagggt ggtcattttc cagaacaggg gggatttcaa catagtcttc tcctttgcaa
    26581 tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag tgacggtgaa
    26641 gccgaagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact tcgccgttaa
    26701 acaaacgggc gacctcactg acaaacgcca accccagacc gctgcttttt tgcccatttg
    26761 cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca ggaataccac
    26821 taccggtatc cagcacctta agggcgacgt gttcctgatc cacttcggcg cttagcgtta
    26881 tgcgaccgct cttgggggta aaatcgatgg cgttatccag taaattcccc agcgcctgct
    26941 ccagtaacgc cggttcagca gcaacgttaa cctcagtagg cataacatgc aaagtgatgt
    27001 ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat gccgccacat
    27061 caacagcagt cagaacgact tcctgacgat tctccagtct tgcctggcgt agtaacgttt
    27121 ctaccaatgc ctgcatacgc gcattttgcg tcagaatgtt gtcggtaaaa cgagccacca
    27181 cttccggtgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc gccagtgggc
    27241 tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc ccttccagct
    27301 tcacgcgcat actttccagc gcctgcgcaa gcttacgcaa ctcgctactg ccgagttcag
    27361 gaagaggaac gggcttattg tcagtgacgg aatcggcata gcgagtgagc cgggcaatgg
    27421 agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc agcaaaatgg
    27481 cgctggccca taaaattcgc cgctcgctgc gcttaatgac cggagccatc gccgcgttcg
    27541 gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc gcggcaacat
    27601 acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca ccatactgac
    27661 cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgcccaacg gctttatttg
    27721 ccgaatcgaa caatactttg ccctgcgcat cggtcatata gacatgatat tcattgcgca
    27781 ctttgttaat gccaccgata ttagcgcgaa acgggcgatg ttgtagctga ttaaacgcct
    27841 gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc agctccgcca
    27901 gcaacgttgc ggtgtcgatc agcgtcccct ccgttgctct tcgcacgccc ggtttaactt
    27961 ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa aaatagccca
    28021 gcaacaaccg catgccgata cgcattacag gctcctcagg ctgtatccca tgccgcgatg
    28081 agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca gcgttttaat
    28141 gtgggtatcg acggtgcgat cgtaggtgtc ctgcgcatct tcccacacga tatccatcag
    28201 ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca ataaaaactc
    28261 ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa
    28321 ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag aacttcttca cccgacgcag
    28381 caaggtgcgc accctagcgc acacttcgcg gggtgaaaac ggtttagcca catagtcgtc
    28441 agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg tcaggaacag
    28501 tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc cactgatatc
    28561 aggcaaacca acgtcgagaa tcatgacatc gggaacctgc tggcgagctt tatccagcac
    28621 cggcaagcct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag
    28681 cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt gcataaatcg
    28741 cctctgttat tgtcgccacg gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc
    28801 ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg cgtcataaaa
    28861 gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata ctacctcgcc
    28921 ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt gctgacaaga
    28981 aatagccgca tcggaggtat cttccgccag acccaatccc cctttaatac cacctgtttt
    29041 cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg cttccacaac
    29101 aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa tctcttctgc
    29161 atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct tgtatttcat
    29221 attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa taacatattg
    29281 attaagaaca aaatctcaat acttttattt ccgtcaagac ctaaaacata ctctactaag
    29341 aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc tgatgtcagg
    29401 tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat tcgcgacctt
    29461 ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa
    29521 gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg ccatgctatt
    29581 ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg
    29641 cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac atttacccgc
    29701 gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc tgaatggagc
    29761 gcctttggta ttcgcccgcc gctgcgtctg ggtgaattca ctatgccaga gcataaattt
    29821 gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg ttcgctggag
    29881 caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt tctcggcaac
    29941 gcgccgacca ttccgccagt gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa
    30001 gacgacgaac aagaggtatt ctataccacc gcgttagccc aggatcaggc agatggctat
    30061 gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa
    30121 ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat acggaacgtg catgccaatg
    30181 ctcaacctga cgcgccgtaa aggtcaggat attgagcgat actacccggc agaagacgcc
    30241 aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattcctat ccgccgttaa
    30301 cgctgcagct catctaatgc aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg
    30361 acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat agaacagtta
    30421 cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact caccagacat
    30481 cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa
    30541 gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc ttcaggaata
    30601 cgcccatcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc ttcggtcaga
    30661 gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg agaatcaaag
    30721 atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt gcgtcctaaa
    30781 tcgctactga tgatatgcgt aatgccaagc tctttggcac gcgtcgccac ctgcatcgcc
    30841 tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg tcgctcggcg
    30901 ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt tttccgtata
    30961 ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca
    31021 ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc ggcgaaggat
    31081 cctgccatat tgcatccatt gccgtcgaga gcggtgtacc ggaacagccc tttggcagtg
    31141 aggaaacgcg cgctggcgca cgaaaccggg tagccaatgc ccgccgttta cttccagagg
    31201 ctgatttttg ggtggcgatt gaagctggca ttgatggcga cagcactttc agttgggtgg
    31261 tgattgaaaa caccacccag cgcggcgagg cgcgttcggc aactttgccg ttgcctgcgg
    31321 tgatattgga aaaagtacgt gaaggtgagg cgcttggtcc cgtgatgtcg cgttataccg
    31381 gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc gggaaactca
    31441 ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt cataatgcgg
    31501 tgtattaagc gcctgatgcg acgctgccgc gtcttatcat gcctaccaaa catattgaaa
    31561 ttacgggtat ttgtaggacg gataaggcgt tcacgccgca tccggcattc ggtgcacgat
    31621 gcctgatgcg ccacgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
    31681 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
    31741 tcgcgatgcc tgcgccgagt tcatttttta actcacgctg gctcatttcg ccgcgcaaca
    31801 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    31861 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    31921 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    31981 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    32041 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
    32101 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
    32161 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
    32221 acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt tataagctgc
    32281 tgaggagaaa atacggttat taccaaattg ctgataaaca tattgcaggt aactggtgcc
    32341 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctgctataac caggaataga
    32401 gaacatcttc accgtatggg tcgccgtgcc cggcataatc tgcatcaggc cgctggcccc
    32461 taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg ccatcgcata
    32521 gctttgcggg atctccttcc cactggtgta acgtttgaaa agatcgttgt aagccagcgg
    32581 gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa cgctaagatc
    32641 ccaccattgg ttgttgaaag cataccgcgc cagttgagcc tgctctgttt ttgacttgct
    32701 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    32761 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    32821 atcaatcttc agctcatact cttcgccgat gcgttgtgca gcaaccatcg ggtagaaacc
    32881 acgctgttgc atgagttgat gcaaaatctc ttttgcttca gcttcacgtc cgcgttccag
    32941 caataaatcc gcctgccagt aacgccattc atctttctct ttcgcttcca taggcagacg
    33001 cgccagccag gtattcaggc cgcgacgatc gccagtgcca agcgccattc gcacacggcg
    33061 ttcaataagc gaagtagatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
    33121 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
    33181 atcttcatta agctgctgcg cctgggcaag cgatgggatc atcagccgtg cattttcagc
    33241 atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa aatcggtcgc
    33301 tccggttgta cgcgcgaagg tcagtaccgt attggggttg ttcgccagtg aaatgattgc
    33361 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
    33421 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
    33481 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    33541 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    33601 gtagtaatta cattgtgctt cggtagttcc gggcttttcc gggctaaagg ctaacaggcc
    33661 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    33721 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    33781 gtcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
    33841 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    33901 acgactacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    33961 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
    34021 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    34081 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgagggtg atcgaccggc
    34141 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    34201 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    34261 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    34321 attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc tgctgcgcat tatggcgggc
    34381 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttatctg
    34441 ccgcaggaac cgcagctgaa cccggaacac accgtacgtg agtccattga agaagcggtt
    34501 tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    34561 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    34621 cacgacggtc ataatctgaa cgtacagctg gagcgtgcgg cggatgcgct acgtctgccg
    34681 gactgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt ggcgttgtgc
    34741 cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    34801 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac cgtggtggcg
    34861 attacccacg accgttactt cctcgataac gttgcgggct ggatcctcga acttgaccgc
    34921 ggtgaaggta ttccgtggga aggtaactac tcctcctggc tggagcagaa agatcagcgt
    34981 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    35041 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    35101 gaagaactga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    35161 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctatggc
    35221 gatcgtctgc tgattgatga cctgagcttc tcgatcccga aaggagcgat cgtcgggatc
    35281 atcggtccga acggtgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    35341 ccggacagcg gcaccatcac tttgggtgaa acggtgaaac tggcgtcggt tgatcagttc
    35401 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatc
    35461 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    35521 ggggttgatc agggtaaacg cgttggtgaa ctctccggtg gtgagcgcgg tcgtctgcat
    35581 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    35641 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    35701 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat tctggattac
    35761 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ccgagtacga agagtacaag
    35821 aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta tcaagtacaa gcgtattgcg
    35881 aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta tccggcctac aaaaccagca
    35941 atttcaatac gttgtaaaac tgtagacctg ataagacgcg gtaagcgtcg catcaggcat
    36001 tgagcaccga atgccggatg cggcgtgaac accttttccg gtctacaaca gccttagcat
    36061 cctcaatttc gtggcgggcc acccgtgcag gttttttcaa accttgttta atctcgacag
    36121 cctcttccag agaggttttc aactcatcaa aaaaactcat tttatacctc gcctttgagc
    36181 agtttcatca cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    36241 ggaaacggct gtcgtaatct tcctcttcaa cccgcacaaa ttcgatattg ttctcttcca
    36301 gcatctccac cagcagattc tggaactctt tacgatccac cgaactgccg aggctgcgta
    36361 aaccatccac cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    36421 catcaatcag cgcctgcacg aacggatgtt cccgcccttc gtactttttg cagaacgcct
    36481 gagtggtgac aaaatcggta tcgataaatg ccactttatt ggcatatttc accgcaaaat
    36541 caatgtattg agcgtggccc agcgcgattt tatcgtagtc agaatactgc aatgcgatct
    36601 catcaccgcc gaggtgtgaa aagacataat cgcggccata ttcccacgca ctggtggtgt
    36661 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccgccaagga
    36721 tcgccacggt acgcacaaaa aacggcttca cttcggtagg aatatattcc cagtagcgga
    36781 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    36841 gcaccgtctc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    36901 agatcagatc cggctggatc cccttttcag ccataaactt tttgataccg ttgctccaca
    36961 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    37021 ttttctgata tttaaaagtt tgcaataacc agcgcagacg atccggcacg gtcggctgct
    37081 gcgacatggc actgtcttcg aacaacgcgc ggtcacgggt atcgtcaaaa cccataatga
    37141 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    37201 gtgggtagaa cttaccgaat acgacaccga tcgttttctt ctgccgggga aactcaagcc
    37261 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttgatt ttggcattca
    37321 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    37381 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    37441 ttctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    37501 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    37561 accgtgtttc gccgcttcag agattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    37621 tgccagcccg acttcaccaa acaccaccag atcctgcggt agcggtctgt cacgcaggct
    37681 ggaaaccatc gccagcagta acgctaagtc ggcgctggtt tcggttacct ttacgccgcc
    37741 gaccacgttc acaaacacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    37801 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    37861 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    37921 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    37981 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    38041 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcgccatc
    38101 taaaagcacc gaacagtcga tacagtgttc cagcactttc gggccagcca gcgaaccatc
    38161 tttggttacg tgccccacca tgacaatcgc cacaccgcgc gttttggcga agcgtgtcaa
    38221 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgactgta catccgccat
    38281 atgcatcacc tggatcgagt caattaccat cagcttcggt tgctcttctt cggcaatcag
    38341 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    38401 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccggtgacat acagcgtttt
    38461 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcaccagg
    38521 gttaccgcca atcagaatgg cacttcctgg caccacgccg ccgcctagta cgcggtcgaa
    38581 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    38641 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    38701 aggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    38761 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    38821 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    38881 cttctgattc aggctgcctg agaggatgca gaataccccc atcaggtcag cgtgacggat
    38941 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    39001 cgctttgatc atcggcaggt cattggctcc atcgccaatc gccacggtct gcgccagcgg
    39061 gatttcatac tcctgcgcga ggcgagtcag agttttcgct ttgtactgcg cgtctacgat
    39121 gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    39181 ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag aaagtaaagc cgccggaggc
    39241 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    39301 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    39361 cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    39421 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    39481 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    39541 tcccagcggc gcgacatcca gctgcgcttc gtgggccagg cgtgtagccc gtgcggtgag
    39601 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    39661 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    39721 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    39781 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    39841 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    39901 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    39961 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    40021 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    40081 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    40141 atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    40201 gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcacgttct
    40261 ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg gtaaaaaagc aggcggctat
    40321 tttaaccagc agattgtcga gccaattgcc gggaaaaacg gaccgctcgg ctatctgcgc
    40381 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    40441 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    40501 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    40561 gtgccggaag aggaagaaag cgagaaaaaa gagtgcccca ttactacaag aaaggaaatc
    40621 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    40681 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    40741 aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    40801 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    40861 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    40921 atctccacgt cgattgtgct caatgcactg aatgcgctcg gcgtcagtgc cgaagcgtcc
    40981 ggacgtaacg atctggtggt gaaaaccgcc gaaggcgacc gcaaagtctc aggatcggcc
    41041 tatcgcgaaa ccaaagatcg tggcttccac cacggcacct tgctgctcaa tgccgacctt
    41101 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattact
    41161 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatccc ccatgagcag
    41221 gtttgcgagg ccataaccga ggcctttttc gcccattatg gcgagcgcgt ggaagcggaa
    41281 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgccag
    41341 agtagctggg agtggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    41401 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    41461 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    41521 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    41581 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    41641 ctcgctgatt aaaatatcca aggaaggaaa tatgatcgat ctggaaaacc aggaacggga
    41701 gattatcaat ttaatgttca gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    41761 taaattatcg cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaaca
    41821 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatggcag aaatttatgg
    41881 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    41941 ttcgtgaata gatacttgaa tggatttta
//
LOCUS       DAZFTN010000028        41963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFTN010000028 DAZFTN010000000
VERSION     DAZFTN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHKV2_001689"
     CDS             <1..266
                     /locus_tag="ACHKV2_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Shufflon protein B"
                     /protein_id="HFO6939589.1"
                     /translation="PTCQSGTWKSSSASIWTNIKTFTLYPKNTQVLGRFKLCINTYRI
                     DGREMAETEVIPIDMPDSNGEMTWQAKNYTQYSSYFMKITCLK"
     gene            complement(802..1086)
                     /locus_tag="ACHKV2_001690"
     CDS             complement(802..1086)
                     /locus_tag="ACHKV2_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilV"
                     /protein_id="HFO6939590.1"
                     /translation="MLTSKEAKNNANNHSLQACQSGRWSGGNKINYSACNWYKSSVAM
                     NHFIGGKSGGSIYYKPIQCPTGYIMTGTRMYGIGDGVDEEHVDAYCCPFN"
     gene            1085..2239
                     /locus_tag="ACHKV2_001691"
     CDS             1085..2239
                     /locus_tag="ACHKV2_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6939591.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTINEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2390..3214
                     /gene="traE"
                     /locus_tag="ACHKV2_001692"
     CDS             2390..3214
                     /gene="traE"
                     /locus_tag="ACHKV2_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO6939592.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYYGVILRQKKEINGSVIYPALSLS
                     PTILNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3300..4502
                     /locus_tag="ACHKV2_001693"
     CDS             3300..4502
                     /locus_tag="ACHKV2_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6939593.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4562..5146
                     /locus_tag="ACHKV2_001694"
     CDS             4562..5146
                     /locus_tag="ACHKV2_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFO6939594.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5541..5999
                     /gene="traH"
                     /locus_tag="ACHKV2_001695"
     CDS             5541..5999
                     /gene="traH"
                     /locus_tag="ACHKV2_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFO6939595.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            5996..6814
                     /gene="traI"
                     /locus_tag="ACHKV2_001696"
     CDS             5996..6814
                     /gene="traI"
                     /locus_tag="ACHKV2_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFO6939596.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            6811..7959
                     /gene="traJ"
                     /locus_tag="ACHKV2_001697"
     CDS             6811..7959
                     /gene="traJ"
                     /locus_tag="ACHKV2_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO6939597.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            7956..8246
                     /locus_tag="ACHKV2_001698"
     CDS             7956..8246
                     /locus_tag="ACHKV2_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6939598.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIYSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8261..8812
                     /locus_tag="ACHKV2_001699"
     CDS             8261..8812
                     /locus_tag="ACHKV2_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFO6939599.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            8902..12669
                     /locus_tag="ACHKV2_001700"
     CDS             8902..12669
                     /locus_tag="ACHKV2_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO6939600.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVLWDKEHKLWF
                     ARPGADLSMLDRWLPRPQEVSMNGSDPVTEFAQVLENAGLVLKELPAMDGKIHRVPTT
                     DDKNGQKSGAYRGFLDGRPAGWYRDYRSADDSPVNWTFSGGEQTDPRARLHLKAHSLQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRINDKNELVIP
                     FRNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDAGNMIAVAEDARQKWKQSPFIFCADNDHARQVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPTASSSNI
                     ADETLSFTSHATENNGKDERHPDNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNELQQESGTSPEIPAPAAAPAAPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPF
                     LPESDGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGPVQADESELQATTATIE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGLFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            12687..13034
                     /locus_tag="ACHKV2_001701"
     CDS             12687..13034
                     /locus_tag="ACHKV2_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6939601.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            13031..13723
                     /locus_tag="ACHKV2_001702"
     CDS             13031..13723
                     /locus_tag="ACHKV2_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO6939602.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            13734..14717
                     /gene="traN"
                     /locus_tag="ACHKV2_001703"
     CDS             13734..14717
                     /gene="traN"
                     /locus_tag="ACHKV2_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFO6939603.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            14720..16009
                     /gene="traO"
                     /locus_tag="ACHKV2_001704"
     CDS             14720..16009
                     /gene="traO"
                     /locus_tag="ACHKV2_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO6939604.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGVGYIAFSKISGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            16009..16713
                     /gene="traP"
                     /locus_tag="ACHKV2_001705"
     CDS             16009..16713
                     /gene="traP"
                     /locus_tag="ACHKV2_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFO6939605.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            16713..17240
                     /gene="traQ"
                     /locus_tag="ACHKV2_001706"
     CDS             16713..17240
                     /gene="traQ"
                     /locus_tag="ACHKV2_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO6939606.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17291..17695
                     /gene="traR"
                     /locus_tag="ACHKV2_001707"
     CDS             17291..17695
                     /gene="traR"
                     /locus_tag="ACHKV2_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFO6939607.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGALLVAGPEMMSRSQKQLGISTISIG"
     gene            17759..17947
                     /gene="traS"
                     /locus_tag="ACHKV2_001708"
     CDS             17759..17947
                     /gene="traS"
                     /locus_tag="ACHKV2_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFO6939608.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            17931..18731
                     /gene="traT"
                     /locus_tag="ACHKV2_001709"
     CDS             17931..18731
                     /gene="traT"
                     /locus_tag="ACHKV2_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFO6939609.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            18821..21865
                     /gene="traU"
                     /locus_tag="ACHKV2_001710"
     CDS             18821..21865
                     /gene="traU"
                     /locus_tag="ACHKV2_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFO6939610.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            21865..22479
                     /gene="traV"
                     /locus_tag="ACHKV2_001711"
     CDS             21865..22479
                     /gene="traV"
                     /locus_tag="ACHKV2_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFO6939611.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22446..23648
                     /gene="traW"
                     /locus_tag="ACHKV2_001712"
     CDS             22446..23648
                     /gene="traW"
                     /locus_tag="ACHKV2_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFO6939612.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            23677..24261
                     /gene="traX"
                     /locus_tag="ACHKV2_001713"
     CDS             23677..24261
                     /gene="traX"
                     /locus_tag="ACHKV2_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFO6939613.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24358..26526
                     /locus_tag="ACHKV2_001714"
     CDS             24358..26526
                     /locus_tag="ACHKV2_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO6939614.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            26600..27250
                     /gene="excA"
                     /locus_tag="ACHKV2_001715"
     CDS             26600..27250
                     /gene="excA"
                     /locus_tag="ACHKV2_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO6939615.1"
                     /translation="MQMVKTINRYPAWWTLYYALRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(27322..27531)
                     /locus_tag="ACHKV2_001716"
     CDS             complement(27322..27531)
                     /locus_tag="ACHKV2_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO6939616.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            27923..28099
                     /locus_tag="ACHKV2_001717"
     CDS             27923..28099
                     /locus_tag="ACHKV2_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939617.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(28164..28259)
                     /gene="dqlB"
                     /locus_tag="ACHKV2_001718"
     CDS             complement(28164..28259)
                     /gene="dqlB"
                     /locus_tag="ACHKV2_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO6939618.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELVD"
     gene            28758..29009
                     /locus_tag="ACHKV2_001719"
     CDS             28758..29009
                     /locus_tag="ACHKV2_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939619.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRGNETIPDTLH"
     gene            complement(29081..29233)
                     /locus_tag="ACHKV2_001720"
     CDS             complement(29081..29233)
                     /locus_tag="ACHKV2_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6939620.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            29525..30733
                     /gene="trbA"
                     /locus_tag="ACHKV2_001721"
     CDS             29525..30733
                     /gene="trbA"
                     /locus_tag="ACHKV2_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO6939621.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            30752..31822
                     /gene="trbB"
                     /locus_tag="ACHKV2_001722"
     CDS             30752..31822
                     /gene="trbB"
                     /locus_tag="ACHKV2_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFO6939622.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPPEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            31815..34106
                     /gene="trbC"
                     /locus_tag="ACHKV2_001723"
     CDS             31815..34106
                     /gene="trbC"
                     /locus_tag="ACHKV2_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO6939623.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMARLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(34143..36842)
                     /gene="nikB"
                     /locus_tag="ACHKV2_001724"
     CDS             complement(34143..36842)
                     /gene="nikB"
                     /locus_tag="ACHKV2_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFO6939624.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTD
                     TDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(36853..37185)
                     /gene="nikA"
                     /locus_tag="ACHKV2_001725"
     CDS             complement(36853..37185)
                     /gene="nikA"
                     /locus_tag="ACHKV2_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HFO6939625.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            37419..37754
                     /locus_tag="ACHKV2_001726"
     CDS             37419..37754
                     /locus_tag="ACHKV2_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO6939626.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(37840..38688)
                     /locus_tag="ACHKV2_001727"
     CDS             complement(37840..38688)
                     /locus_tag="ACHKV2_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6939627.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            39037..39234
                     /locus_tag="ACHKV2_001728"
     CDS             39037..39234
                     /locus_tag="ACHKV2_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939628.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            39268..39519
                     /locus_tag="ACHKV2_001729"
     CDS             39268..39519
                     /locus_tag="ACHKV2_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6939629.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(39550..39747)
                     /locus_tag="ACHKV2_001730"
     CDS             complement(39550..39747)
                     /locus_tag="ACHKV2_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939630.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGALEVAREIACRLRDMGWTPE
                     RIQEATGLSGEELKKLFPDEQ"
     gene            complement(39770..40684)
                     /locus_tag="ACHKV2_001731"
     CDS             complement(39770..40684)
                     /locus_tag="ACHKV2_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6939631.1"
                     /translation="MSKKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(40749..41015)
                     /locus_tag="ACHKV2_001732"
     CDS             complement(40749..41015)
                     /locus_tag="ACHKV2_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939632.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(41109..41543)
                     /locus_tag="ACHKV2_001733"
     CDS             complement(41109..41543)
                     /locus_tag="ACHKV2_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO6939633.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
BASE COUNT        11101 a        10815 c        10180 g         9867 t
ORIGIN      
        1 ggcctacgtg ccaatccggt acctggaaat catcatcagc gtcgatatgg acgaatataa
       61 aaacatttac gttatatccc aaaaacacac aggtcttagg gaggtttaaa ctgtgtatta
      121 acacctatcg tattgacggg cgtgaaatgg cagaaacaga agtcattcct attgacatgc
      181 ctgactctaa cggcgagatg acttggcaag ccaaaaacta cacacagtat tcgtcctact
      241 ttatgaagat tacttgcttg aaatagctta ttggcaaatg gcatatacag ttattgaacc
      301 attctgatct tcggttgtat cgcacgcagc cgaccatgag ttattggctg atgggaatga
      361 cctggtaagc catatgtaac cagttcgtga ggtgcatatt ccgccgccac ctgtaacacg
      421 ttttccagtt ggacaggtag ctgttgctcc aggaaagcgc caacctgtgg cctgtgctgt
      481 agcaatttga agctcaccag atcctacctt tctccaggta ccggattggc acgtaggcca
      541 gtgccaatcc ggtacctggg gcgctcctaa aattcaattc acaacgcaga cctacaacat
      601 agctaaaaac actcgtaatt taaggctcgg ggtccatgca tattgctcat ggacttacct
      661 gaatggctca ccctttggtg gttttcaaca ggtatattcc gaccaaaaca acgtttggta
      721 tgtgagtaat tatgcttggg gaaattatga gtctggtggg accatatcag tcacatgcct
      781 caatcttcct ggtgctggag cttaattaaa ggggcaacag taggcatcga catgttcctc
      841 atcaacacca tcaccaatac cgtacatgcg tgttccagtc ataatgtatc cagtcggaca
      901 ttgaatcggc ttgtaataga tcgaaccacc actcttaccg ccgataaagt gattcattgc
      961 tacggatgat ttgtaccaat tacatgctga gtaattaatt ttattacccc ctgaccaccg
     1021 accggattgg cacgcttgca gagaatgatt gttggcatta ttttttgcct cttttgaggt
     1081 tagcatgccg tctccacgca tccgtaaaat gtccctgtca cgcgcactgg ataagtacct
     1141 gaaaacagtt tctgttcaca agaaagggca tcaacaggag ttttaccgga gcaatgttat
     1201 caagcgatat cccattgccc ttcggaatat ggacgaaata acaaccgttg atattgctac
     1261 atacagagac gttcgtttag cagaaatcaa cccccgaacg ggtaaaccca ttacaggtaa
     1321 tactgtacgt cttgaactcg cccttctgtc atctctgttc aatattgctc gtgttgaatg
     1381 gggaacctgt cgtactaacc cggttgaact ggttcgcaag ccgaaagtat cctcgggacg
     1441 agatcgccgg ctaacgtctt cagaagaacg tcgcctttct cgctatttcc gcgaaaaaaa
     1501 tctgatgttg tatgtcattt tccatcttgc ccttgagaca gccatgcggc agggcgaaat
     1561 actggcctta cgttgggagc acattgattt gcgccacggt gtggctcatt tacctgaaac
     1621 caaaaacggt cactcacggg atgttcctct gtccagacgt gcccgtaact ttcttcagat
     1681 gatgcccgtt aatctccacg gcaatgtttt tgattacacc gcatccggct ttaaaaatgc
     1741 ctggagaata gccacacaac gacttcgcat cgaggacctg cattttcacg atctacggca
     1801 tgaagcaata agccgcttct tcgaactggg tagcctgaat gtaatggaga ttgctgcaat
     1861 atcaggacat cgttccatga atatgctgaa acggtatact catcttcgtg catggcaact
     1921 ggtcagtaaa cttgatgccc gccggcggca gacacaaaaa gtggcagcat ggtttgtgcc
     1981 gtatcctgcc catatcacga ccatcaatga agaaaatggg cagaaagcgc atcgtattga
     2041 gatcggtgat tttgataacc ttcacgtcac tgccacaacg aaagaggaag cagttcaccg
     2101 cgccagtgag gttttgttgc gtacactggc cattgcagca cagaaaggcg aacgtgtccc
     2161 atctcccgga gcgttacctg ttaacgaccc tgactacatt atgatttgcc ctctgaaccc
     2221 gggcagcacc ccgctgtaaa catcattcaa aacacataca gaaaggcgag ttgcaacaac
     2281 tcgccaaaca cattcgatct gcatgatcga aattattcgt ttcaccagaa aaaacaccga
     2341 agatacaata accacatgag ctgaaaagac tttttcagtc ggaggaacaa tgagactgaa
     2401 tacgaccggt attgcggccc gaatgatgct ctcgctggac aaaaaagcaa tcggcgaaaa
     2461 actcatcaac ggtcgtcaga taaacccgac acctctgcag gtccgttacc cccaaggggt
     2521 aagagaagtt ctggggataa tgagcgaaca attagccata tccaccgctg atttaacccg
     2581 aatcctgctt gaggatgctc ttcataatat gtttatgcct gcggataaca ccacaggaaa
     2641 cataataagc agaattgaat acatcatgct gtcgcatgat ataaatgcgc cattactggc
     2701 aacattgctg tcgccatgga atatccgctc cacggttata caggacccgg ccagacttgc
     2761 agactatctt tcagctgacg ctctcgaaca tcttgctgaa tgttttaatc tcaatccgga
     2821 ctggctgaat ggccatgaaa actatccgat agcgttgtcc ggtgaatggc cagatactgc
     2881 tgataatttc agaatgctta ttaatgatag cagcaacact gaagttattt tctggcatag
     2941 tttccccttc gcaggaaaca cgaaaagaga atattacggg gtaatattga gacagaaaaa
     3001 agaaataaat ggttctgtaa tatatccggc actatcatta tcaccaacaa ttctgaacga
     3061 tgaaaaaaga aaatggttaa ctgaatatac aaccaggcag aatacaacaa tgtctttgcg
     3121 acgcgtcacg ttgagacctg gccttgccgg gaaccttatt acagggcaaa tactacctgt
     3181 tagcttattt aatacgtctt tattaccctg gtaaatacca gatagtacca aagtaaaaaa
     3241 ttacggcaca tccgtgtcat agcgttgttt tgtctaaatt ctgataaaca ggaaattaaa
     3301 tgatgaagaa aaatcatatt acccgcacca tcattgcatc tgctgtatta ttttccttta
     3361 acgctgcagc agcgacgtca tattttgaag cacgtaacga tgcaatgggg ggcaccgggg
     3421 tagcatcttc ccattatggt gtggcacctc tggcaaaccc ggctttactg acaaagcaca
     3481 acagcaatga tgatttcagc cttcttctgc cgtctgttgg ggcacaagtt gctgatcctg
     3541 atgatgtatc aaataaagct gatgatgtta aagatgactg ggatttgttt gatagtgctg
     3601 ttgataacca gcatggcgta caacaggcag cagcaaatct taaacatcgt ctgcaggaat
     3661 tccgcaatat caatgcagat gctcaggtag gtgtatcagc tgtagcagcc atggcaaatg
     3721 acactctgcc gtttgccctt atggtcaagt cttacggaac tgtcagcgta aacggaaaag
     3781 taaacgatgc agacctggat tatctggaca aagttgccaa tggaacaatt accgacgtcg
     3841 ataagaatgc cctgacctca cgcgcattcg ggcgtgcagc tgtaattacc gacgtcggaa
     3901 tttcatttgc caaagaactg gaaacggccg gacaaaaatg gtctcttggc gtcacaccaa
     3961 aatatcagcg tgtggacctg tttaactaca acgtaacggt tcgtgattac gacaaagacg
     4021 attttgacgg tgacaagtac cacaacacga aaaacggatt caacgccgat attggtgctt
     4081 atacagacct gaatgacaac tggactgtcg gtcttgttgc ccagaacatc attccacgca
     4141 gtattgatac taaagtcgtc aatggcttta aagaaacctt caaagttcgt ccacaggcaa
     4201 ccgccggtgt atcctggcat aatgacctgt ttaccactgc tcttgacgtt gatctgactc
     4261 ctgccagtgg cttcacttct gacagcaaac gtcagtttgc aagtgttggt gctgaattta
     4321 acgcatggaa atgggcgcaa ttgcgtgccg gttatcgtca gaacatggca tcaaactccg
     4381 gaagcgcctt caccgctggc gttggtatct ctccatttga cgtcgtacat atcgatgttt
     4441 ccggcctggt aggaactgac cacgattacg gtgcaatggc acaacttcag ttcactttct
     4501 gattactgtc cccgcccccg gcactaaacc cgggggtatt tttatattca gtttaaataa
     4561 catgatgaaa ccacgttctt cttattcaaa aacagcattc attcttttat tttctgtttt
     4621 cctggtagcg gcagtgacaa aagcaaaaag ctcgcttccc gatatcaccc tggaacaggc
     4681 aaaagaaatt aatgctgata acaccgttat tttccttttc cgccatggag aacgttgcga
     4741 ccgttcagat atgccatgct attcggataa aagcggaatc actattaccg gtacagagaa
     4801 agcacagcag gaaggtatta agttcgcaac aatcttcagt gaatatgata tttactccag
     4861 caatgcggta agaaccatac aaactgcaaa attcttctcc ggtaaagaac ccgtcgttat
     4921 ggacagttta tcagactgca ataatgatct ttataagacg cttgaatcaa tcgcaagaga
     4981 atctcataaa aggaacatag taataatgac acataatcat tgtttatcat ttctggccag
     5041 agataggctc ggcaagaaat tcaaaccggc gtaccttgat gccctgatca tgcattacga
     5101 tggtacccga ctcatacttg acggaaaata caacaaagag gcatagagga tatttgaatt
     5161 tacataaatt ctgatatact cagtaagata caagagtgta cgggcacatg tgcctgtctg
     5221 aattgcaggt tatccggacg agggccggga actgtttcag ggacaagcgc cggtacgttc
     5281 gattcataaa tcgtcgagtc atccatcatg gatcgattcg gcgcggagcg ttaaagatta
     5341 cgctcatgtt cgttttatgt ctgacgacac atcaaccagc cagtatcagg aataatcacc
     5401 ttcagtaaac tatgaggcta tttacctggc cacactggca tttatttccg gtaacgcaag
     5461 tatagccgga aacaaacatc atcctgtctg taccggagca ccggtatatc ccattctgat
     5521 ttaactgctc aggaattatt atgaaccgta ttatacctgc ggggtgtctt gccgcccttt
     5581 ttctggctgg ctgcaactcc ccttctcagc atactcctgt tccgcgtaac tacggtgctg
     5641 cctctgatgt ggcggttacc cggcacactc agtacacatt atgggacaaa ggtgtgtttt
     5701 ccggccaact tccggtttca acggggtctc atcttctgac agctaacagc gatcgccttt
     5761 cctttgactg ggaaggtgat gcaattgaat tactgaacga actggccaga gtcaggggga
     5821 tgcagtttaa ctacaatggt gtccgcctgc cgttaccagt caaccttcac gtccgggata
     5881 tgacgttttc caatacgttg agacttattg aagcccagac agcctggaga gcaacaatcc
     5941 atcaatatcc gggcttgtta caggtcagct tcatgcagcc ggagaaccgg aaaaaatgaa
     6001 agcactcacc tctctcattt tgccagtaat actgcttact ggttgttctt cttcccctac
     6061 catctcgcca ggtggtgtca atacgccacc acctgacatt aatgcatggc ttaaaccacg
     6121 aagtgaaaag ccggatagtc tgtcagaaac acgctggaaa atgctgaccg acgcaggtaa
     6181 aacgcttgga tttcgtggtg gcaaggctca gcgttcatgg gagctgattc aggcactcaa
     6241 cgcccgggaa agcacactta atgccttata cgatttccgg ccactgatta gcccggaagg
     6301 ctggctacca ccggttattg atgaagcaca ggacgtagct catatcacgc cagatcaaat
     6361 ccggacctct tcccgggtct ggaccatcat tcgccctgaa cgctttgtca gcaatccacc
     6421 gggctggcgt gactggcttc tccgggggct ttcaacaaca gccactccgg gtacagaagg
     6481 cagcgtcgtt ccagaagaca gcgtgcaacg aaaagtgtgg gaaaccgcat tacggcaggg
     6541 atggcaggaa ggaagacaaa acgccgacct gacgcttgaa gccaaccaga aaaccctcac
     6601 ccgtgattac cggggcatga tgctgtactc acttctctgg cgtcagggca tgatcacccg
     6661 tccggatgtt tctgatcaaa tgcaaaccgt tacaggtgac gggaaaaaac tggtcactgg
     6721 agatcgtgtt cgtcgcctta aaaatcatgc ggaattcaat cttcagaaat ctcactggcg
     6781 tccgcttata ggcactgaag gaggctcccg atgaatattc ctgatgagtt tggtcttttt
     6841 ccgtttcagc gtttcactgc agatgaacta cggcatttct ttgtctggtg tgccgcacat
     6901 aaagtcagtg atgtggatct gaccggcggg tcacctgtat ctgtcagtcg ctttggccga
     6961 cgggtgcgtt gttccagtgc aacgttacca accacactca tgtcctccct gattgatgaa
     7021 ctgttcggtc gggaagtcat tccccgcgtt ctggcaggca atccggttga caggacgata
     7081 cagattaacg gggatgcatc cgggcgctat ggtcttaaac gcggagaacg catccggtta
     7141 cgctctcacc tcattcaggg cacatctggt gcggaggaga aagcaatctc aataaccatg
     7201 cgtgtcatcc cgacagaaat accggacatt ctctccatga acattgaacc agacctgctt
     7261 gaagcaatgg tctgcaaaag cggtctggga tttgtatgtg gtgaaaccgg gtcaggaaaa
     7321 tcaacgctgt gttcagcact gtaccgttac attatggaca atttccccga cgccaaaatc
     7381 gtgacatatg aagacccggt ggaatatatc ctgggaaatg aaaacgatct gctcccaccg
     7441 catcaggcag aaatcgggcg tgatgtggtc agttttgctg ccggactgcg ttctgctgta
     7501 cgacgtaatc ccgaaattat cggtgttggt gagatcaggg ataacgaaac cgccgacgca
     7561 gcggtacagg caggcaacac cggacattac tgcctgagca ccatgcatac gaaatctcct
     7621 ggtgaaacgc tggcacgtct tcttgggctg tttccccctg tcatcaggga ttcgatggcc
     7681 tgggctgtcc tgtcactgtt gcagttcatt ctggttcagg ttctggtcag aacaaatgac
     7741 ggtggtcgta aagccgtccg ggaatatatc gtcattaacg atgagcttcg cgacaatctt
     7801 tccggaatgc cacatgcaga gtgggggcat cacattgatg ccataatccg gcaggagaag
     7861 cgccgtatcc gggaccagat tctggaaatg tatatccgga atgaagttga ccgaagagaa
     7921 gcgattcttt ttatcccgcc aggggagtta cggtcatgaa aagagaaggt ttctggcgat
     7981 atgcaacacc gccagtcaac atccagggcg tacctctgcc gttcaccatg atttatctgt
     8041 ttatatgccc ctttccctcc aaaacaacct tctggatttg caccggcatc attttatttt
     8101 tcgttattct cgaccgttca ggctggactg tcaggacgat ctgtactcgc atttacagca
     8161 ttctgagagg ggccatcgcc tccggtcgtc catggtggta tcgccaccat acggaatcac
     8221 cacgagactg gactggcctc gattaaattc tggagtcttt atgtcaggta aaaaaactat
     8281 cgttactctg ctccgggtat cgctactggc atgcccgtta ctgttcacga caccatcgtt
     8341 tgcaatgatc gacacgccat ccgtgaaggt cggcttttct cctgaaggaa gcgcatccgc
     8401 tctggtgctt gacacaatca acagtgcgga aagctccatc aggatgatgg catattcctt
     8461 caccgacccg gatgtaatgc atgcgctggc aaaagcgaaa aaacgcggag tggacgtccg
     8521 tattgttgtt gatgacaagg ggaatacgaa ccgggcaagt caggaagcga tgaaatatat
     8581 caacctgctg gacattcctc tccggactgt agatgccttc cccatccatc acgacaaagt
     8641 catcattgtt gacggaaaca cggttgaaac cggctcctat aacttctctc gtgctgctgc
     8701 acgcaagaat tcagagaatg tcgttgtgct taagaatatg ccggatgtag ccgcacaata
     8761 tcttgaacac tggcaggatc gctggaataa gggtacagac tggagaccct gacaaacatt
     8821 tctcccccga ttttctatcc ccccagccac ccccggcttc cggcggtggt ttttttccgt
     8881 cttttttcag gagaagcact catgccgtct gtatcccgct accgcacctg gcttgccgtg
     8941 cctgcagatg aaatagaaga tctcaaaaaa gcacatcccc cgatgaacgg gcacacgccc
     9001 gtcctctggg ataaagagca caaactctgg tttgcccgcc cgggggcgga cctgtccatg
     9061 ctggaccgct ggctgccccg accgcaggaa gtctccatga acggcagtga tccggtcacc
     9121 gagtttgccc aggtactgga aaatgccgga ctggttctga aagaactgcc ggccatggac
     9181 ggtaaaatcc accgggttcc gaccaccgat gacaaaaacg ggcaaaaaag tggcgcatat
     9241 cgcggcttcc tggacggcag acctgccggc tggtacaggg attatcgcag cgcggatgat
     9301 tcgccggtca actggacctt ctccggcgga gagcagacag atccccgcgc aaggcttcac
     9361 ctgaaagcgc attcgctgca acgcagggag gacgctgagc gggaactgaa agcacagtac
     9421 aaccgacagg ctgcttatgc ccgccgctat gtcaacaaat ggccgcaggc cacggcacat
     9481 gaatacctga cccggaaagg cattcaggct gcgccggggg tgcgaataaa cgacaaaaat
     9541 gagctcgtta ttcctttccg caaccgtaac ggcgcaatac gctcctacca gcgcattccg
     9601 gtcaccggcg ggaaggatgc ccgcatcctg aaagattccg agaaaaccgg taactggttt
     9661 gcgctgggta caccgcgcaa cggccagccg gtgctgtttg ccgagggcta tgccactgcc
     9721 gcctccctgc acgaagccac cggccttccg gtgctgatga cagtggatgc gggcaacatg
     9781 attgccgtgg ccgaagatgc ccggcaaaaa tggaagcaaa gcccctttat cttctgtgcc
     9841 gacaacgatc atgcacgcca ggttaacaaa ggtatcgtca gtgcaacaaa agccgcagaa
     9901 ctgacaggag gatcggtcat ctttcctgcg ttcaccgatg ccgaaaaagc gcagggactg
     9961 accgatttca atgacctgga cgccagccgg ggacgggccg cttttcagca tgtcatcaac
    10021 gcccagttag aacatatcgg ggtttccacc cccaacagta acaccccgga aatccgggag
    10081 gcactggtga tcggcaacct cgtttttaca ccggtccaca ctgaggaaaa aaccatgact
    10141 ccgacagaat atcctgaaac atcacccgat acagggcaca gccatgacca gggaccttct
    10201 ctgccgtccg ccacgcagca ggaacaacca actgcttcca gcagtaacat tgctgatgaa
    10261 acactgtcat ttaccagtca cgccactgaa aataacggca aagatgagcg gcaccctgat
    10321 aacgttcagg cgcaaacgga aagctctgta gttcaggact cccctgcaga atcaccggca
    10381 tccccggaaa gtacaacggc gtctgcccct gatgaacccg ctcatccggc ggaacctccg
    10441 gaaaaagttg tctctgtggc cacagataaa gactggcgtg aatttgaagc cgaactgtca
    10501 caaccggaaa aaaacgagtt acaacaggag tccggcactt caccagaaat accggcccct
    10561 gctgctgccc ccgccgcccc tgaagacagc ccctcatctg aaaccatgaa tgaatcagcc
    10621 gaaccatcac cttcccctga tgcagtcaaa gaggaaatga ccgtgatgac ggagcctgat
    10681 gacaaacaaa gcaccggaat atccccggat actccccggg aagaggcggt cagccatgta
    10741 actgaagaca ttccccttca gccagagccg ttccttcctg agtcagatgg ccctgaagaa
    10801 tacgaagact acagcgcata tcaggagctg atgaacaatg aaaatcctga gcatctgcag
    10861 gaaaacagtg atatgccgtc gcaaccggac acaggccccg ttcaggcaga tgagtcagaa
    10921 cttcaggcca caacagccac tatagaaccg gctgtccggg atgaacccac tccgcagcaa
    10981 cctgttcaga gcacatcacc ttctgataat acttcatcgt ttctcgacaa agcgcgtggc
    11041 ctttttacgc gtaaaaaaac ggattcacag gcgcatgaga acagtgatcc cacgccctca
    11101 gcggaaacca cgacgactcc accgatacct gacagtattg tctacgcccc ggaacgttct
    11161 gatgcaccga tatcgctcaa catggacgaa attcttaaag agcttgactg ggaaaaacga
    11221 gcagacagaa ccgtcctgta caaactggat ggcaaacctg ccttcattga ccgggtaaac
    11281 cggctggaaa tggtcaacgg tgccagcaat gacgatcgca gcgttctggc cgcacttgct
    11341 gtggccacca aattttatgg gggcgtcata gagctgacgg gcagtgatgc ctttaagcaa
    11401 aaagcaatgc agttgattat cgagcacaac atcgatgtcc ggatgaaatt gccagatcaa
    11461 cgggctgaac tagaaaaatt acgcaaggaa atggctgtca gtaaggatgc agttgtcacc
    11521 catcagccaa caccggaact taaccggaat accccagaac aaccggctgt ccctgacccc
    11581 gttcaggaaa aagaagccac acaatccccg gcgtccccag tagcccctga agcatcaacc
    11641 gtcagcaccg cctctgcacc atcaccggct gaaccaggca aaacggcaga tgtggcacca
    11701 gacgaagaac caccaggaaa acttcgcccg ggagagagcg tgactgccgt actgcacaat
    11761 tttggtcgtg ctgaatatgc acccggcaaa ggagagagtt tctttgttga gcttaaaaac
    11821 cgcagtggca gcaaactcta ctggggggaa caactggaaa gtctggtcaa aaaccatcag
    11881 aaaggtgacg tggtgacact gacacttcag aaccgcgaac aattcatcct cccgggagag
    11941 caaaaagcac gtttccggaa taagtggtcc atggagtcag ttaccaacgg gatctcggta
    12001 tcacatgata atccggataa aggtcagcgt attcaggcga ttccggtcga aacctttatg
    12061 aaagtggctg cgcaaatcag ccaggggtgg cccgaagaga tgaaagcgtt acgtatgcca
    12121 gaaaacgtcg gctcacatct gtttatcggt gaagaccgac atccggtctc cgcgccacag
    12181 aacgcaaatc aggtaacaga aataaccagc gcagcccctg acaagctgac acctgttctg
    12241 ggatcagtgg ataaggatac gcgtgaactc aacctgctac ttgtccagag tgccgatgaa
    12301 catctgcagg gtgtggtccg gctgaacggc acattgtatc cggcactggc caccccatca
    12361 gccgacaaca gtcagctggt tatcaatgcg ctgaccgaca aaggtttacg ctttgcaggt
    12421 tatggtgaag cggtaaacca cgatgctgac agcacgaatc gccctgcacc ggagcttatg
    12481 cagtttcatc tgaaaacccg ggaagaaccg ctttttgctg ctgtctacac gccggaaaaa
    12541 caacctgatg cgttgtaccg gaacctgggc ttcgagcagt catggcagca gtggtccaac
    12601 agccaaaagc cggaggacag gcaggaaaaa actctgcatc aggatctctc ccacagcccc
    12661 ggccgatgag ccggtaagga agccatatga acatgcataa gtctctggtt gccgtgctcg
    12721 gccttatcct gatttcatcc acggcaagtg ctgcaacctg tcgtgcgggt agcgcggcac
    12781 aggccggtag taatacagga tatgagcgag cacgtgcagc tgcggatgca tggtcacaac
    12841 gtgaaaacga tgtttcctca tccctgcagt cctgtctgtc acgaatcaaa aaaatcagca
    12901 taaacctccc ccagttcccc agccttgacg acattctgtc gcagctggaa aaccaggtct
    12961 gcgacgcggt cgtggacaag gtgaatgaaa agctgccggg gaatattgat ccctggaagg
    13021 actacaacct atgaccgaaa ataccgagcc atcctcgcca tcacatccgg cacctgaccc
    13081 ggaaatactg aagagcacaa tcagggctat gaagcagtca gaacagcggg caaataatgt
    13141 ccctgcgctg ataaaagcgc ttttatgtac gggcacatgt cttcttatca gtattactgg
    13201 taacgccatt cagtactggc actcaacgaa tgtggagcgc gaatatttcg ctacagacaa
    13261 tggtcgtctg gtcaggctgg caccaacaag tcagccagca tggagtcaga atgatgcaat
    13321 ggcctttggc agccaggcac tggcgaccgc attcaacctg gattttgtgc actatcgttc
    13381 acagatctcc tctctgtctc cgcgtttttc tgacgaaggt tttgccggat atgtgaatgc
    13441 attacaggca tctaacattc tggagacgat caagaaagag aagatgaatc tgaccgccac
    13501 gacaggcgct ggcgttctgg ttcgacaggg acaaatgtcc gacggcgtat ggttctggac
    13561 atttcagtat cctgtccgta tgcgtctggt cggccagaca accagcaagc cagaacagtc
    13621 ttttgttttc gaaatcacca tccagcgtgt tgaccccaga ctgaaaccat ccggtatgga
    13681 aatccggcag atgatctccc gtaacgccgg accgaactcc tgagataagt aatatgcaat
    13741 cacgttattt actgagcaca ctgctcctgg tgtgcagcgc cgccacgtct gcagataacg
    13801 caggatggca gaacgcacgc acaccacaaa cgacaaattc tgccagcgat cacgttcaga
    13861 acgcctccca aaacaccgga ggtgttccgg caacactggt aaaaggtgag cttcctgccc
    13921 ccgggcaggc cagcccgctg gtacaggatg cggcaagact tgattctgag ctctccgccg
    13981 atgagattcg ctcactacgc agtctcatgg cggataacga gcgggccatc aatgcgccca
    14041 tcaccagtgt tgtaccccgt atcagctctc tgacagttaa tctgtccccc ggtgccagcc
    14101 ttcctcttgt tcgcacagcc atgaacaacc tgagcgtagt gacatttacc gatatcaacg
    14161 gttcaccatg gccacaatcc gatcctcctt ataacgcggc accaaaactc tttgatgtgc
    14221 agtacaacga gaacatggtg acaatcacac cattgcggcc atgggcatcc ggaaacattt
    14281 ctgtctacct gaaaggtctg tcggtaccgg tcatcctgaa cgtaaccagt ggcgaaacag
    14341 atacgccgtc atccagtcag gaaatggaca gtcgtctgga tctgcgaata ccacggcagg
    14401 gaccaaccag cccggttgtc tccatcccga cggataaaat cgccctgcat gatgcaacat
    14461 tacaggcatt tctggatggt attcctcccc gggacccttc agttaagagg ctgaaattca
    14521 caggtaatgt cccggataca acgatctggc aacacggcga tgacctactg gttcgttccc
    14581 gggcgatgtt gcgcgatgaa tttgaacaga ccttgtcctc tgccgacgga actcatctct
    14641 ggaaacttcc cgtcacccca ctactgactt tctctgtgaa tggccagtcc gttcacgtga
    14701 caccagaact ggagtaatca tgagtgctga acaggatgcc ggtaaatccg gaaagaaact
    14761 ggctgccctg cttggactgg gcagcatcat cttatttggc gtcggctata tcgcgttttc
    14821 caaaatatca gggagtaaca gcgatatgca gtcagccgtg aacattaaca gtgcagcaag
    14881 cggcggaacg cgcagcgtta ccgaaacgcc tcactatcgt gaactgttaa gagctgataa
    14941 tgaacgaggg gccgcagctg cagcccgcaa taatcagacg ttcattgcca gtcttccaca
    15001 ggggcttgat attccggaca ctccggaaaa gcaacaacag cctgcagcaa aaccggaaaa
    15061 ctatgctcat cgccaggctt ccggcacacc acaggaggat cgagcagcca gtgaaaaacg
    15121 tatggagcgg ctgcaaaagc tgattgtaag aataaaagat cagcatcctg ccggtagtac
    15181 accaaccata gcaacggcta tgtggaataa atctccggca gaaacaacag ggcagaatgg
    15241 tacgcagcaa ttcgcattac agaatgccag cctgtccacg ccggtagcag aaaaagggat
    15301 acaactgata cctgctctga cccgtatacc agcatatatt gatacggcag tggattctga
    15361 taacccgtca tccaaggtta tcgcaaccat tcccgctggc ccctgggcag gggcaacgct
    15421 gttcagtccg ggagtcaaac tggtgggtaa cggcgtggag atccatttcg accggatgag
    15481 ctggaacggc atggacctta aggtcaatgc atatgctcaa cgggaagaca acctgatgtc
    15541 atccgtggcc agcaatgtga atacacgctg gtttaaacat atcattctgc cgtcagtgtt
    15601 aggaggtgtg ggcagtattg gcacacttta taaagatgcc aacacgcagg ttattcaggg
    15661 taactatggc acggttaccg gacgcgtggg aatgcccagt ggtgaagctg ttgcgggcgt
    15721 tattgccggt ggtatggcgg aaagaggaag tcagatatta acccgccagg cagaaagtga
    15781 gccctataaa caggtagaag tgtatcagca tgaagtcgta tcaattctgt ttgttgaccc
    15841 ggtaatgaca aacgacgcca ggtcatcttc cctttccagt ggcatttccc cttcagttaa
    15901 tcgtacatca cagacggaac aacgctcgca ggcacgaatg cagacagcca tggagcaaag
    15961 aaaagctgtg atgcaacgtc ggtatgatga acaaccggag acaccataat gaaacctgaa
    16021 acagaaatag atgaaaccgg cagcttcggt gaacccgaag agaaacccgc ccctttctgg
    16081 aaacgttccg tctgggggat atctgtagca acatgggggc tatgtgcagt cgtattactc
    16141 gcagccatat ggtatctgtt cctgagagca ccatcggaaa caggcatgcc tccgtttaat
    16201 gatgcggatg ctggcgtaca gacctggcaa accacacaag aatcatcacc ttccgttcag
    16261 tccactgaga ccatgactgt ccggacagga gaagatatga gccagcttgc ccgggatgta
    16321 aaaacagagc tggataaccg cgatgaaaaa attcaggcaa cactgaatat gcttcacgac
    16381 agcattaaca agctgggcga agctataaaa aaggatgaag aatacgcaca ggaaacgcgc
    16441 cgtcagctgg atgatatccg gagccacctg aacggaatga tgacacagaa aagcgtcacc
    16501 gaatcctctt ccacaccgca tccagcaaag aaaaaaacgt cgtctgtact taacggaatg
    16561 aaaatcatgt cgatggaaac tggcatggcc tggattcgct ggcagggtag tacctgggct
    16621 gtcagagaag gtcagactct gggcaatgtg gtgatccagc ggattgatcc gacgacccgg
    16681 accatcatca ccagtgcagg gacgttacgc tgatgaacat ggatgccctg accgccattg
    16741 agaactttgc cagcagtatt ttcagtgccg gaatggattt cctgtttacc tggggagaat
    16801 tcatcggggt gatcagcatg attaccctgt ttgcccgcgc ccgttcagcc ggtccggtaa
    16861 aaatgtctcc cggaaaattt attgcgggga tgttaaccag ctgcatgctc gtatcactcc
    16921 cggcaatgat taacgccgga ggcgtacaaa tgggattcag ggctgactca tttggcccga
    16981 ttgcatatgt acaacctcag acgtttggcg ctgctgctgg cgcagcaaat gcagttctgt
    17041 cgctggccaa gctggccggt gttggatttg taatgaacgg gatatccatc tggcgcaagg
    17101 ccggactcga tggacatacc gcactgagtg ccagtgaatc cgtcagtaag ggaaacgtca
    17161 aatttattgc cggcgtcctg ctggtcttta tcgatcgtgt cctcaatgcc ctgctggcaa
    17221 gcattggaat cgtcttctga tttttttcac cttcatataa accactgata tatccattta
    17281 aggagaaact atgtcacgta ttcgtgtcgt ggcggcccgt ctgtacgccg catttatcca
    17341 cgcacaactg tttgtaatgt catgccggag caaactgact aagttcctgc tgctcattcc
    17401 catgatgttg ctgcccaaag ccgttcttgc cgatggagat cttgccgata tggttcgcaa
    17461 tgtcgaacaa ggtgccaaaa cagcacagtc atcatcactg actattgcgc agttcatcgg
    17521 cgtaattctg tttctcgggg gcctgattgg cctgaaaaaa gttgggaaac aaggggggat
    17581 gggactggct ggttgcattg tatctatcgt cattggggcg ctacttgtcg ccggacctga
    17641 aatgatgagt cgcagccaga aacagctggg gatttccaca atttccattg gctaatcttg
    17701 tccagggcac tggcatttat ccggtgccct tcctgttcgg caatacaagg aggcacatat
    17761 gtctgcctgg agcccggagc acataacgtt acgacgaaca gaaaacggca atgtgattgc
    17821 ccacgacagt cgggacgatc aggaattcat ttttgcagaa tgctgttccg atgaaatcgc
    17881 aaacatcatt ctggaagccg tgaaacgtta caccagtacg gagcctgctc atgcaacaca
    17941 gcattaaaga cttgtggttg tatccctttc cagaaattga tgtggttcat acacaggagc
    18001 cactgttacc ggaaccggaa ttaacaacgc ccggtcgatg tatctgctgc cggcaaaacg
    18061 tcaggcaccg tttcagactg gatgactcat ggccactcag acagcttacc gacacaatca
    18121 gtgacacacg agttcgtctt aataaggcca cagaacatct ggataagtta aaaaagcgtg
    18181 gtgaaccagt cgcaaccggc gaaaaggaaa agtacaacac ggcagtaaaa gccgctgaac
    18241 gggcgcttga gcaggcccgt ctgtctgcca gacgtctttc actccgccat gtacagaaag
    18301 ctgaaatcac cagtacagag tcgttgtcag agaaagagca ggaactgttt catgaagacg
    18361 gcccgcctta cagcttatgt gctttctgcc acgcctggca ttcactgaat ggttatgccg
    18421 cagcacaggg cgttatggtg tggctacccg accttcatcc atccacggtc gtggccctta
    18481 accgtcgttc attacaggaa gttttcagta atgataagtt ccgggtccgg cggggacggg
    18541 aagcgctctc tgcactgatg caaaacagac tggcggttga ggataaattc cgcagtttcc
    18601 gcccggccga tttcgctgac gtttttcgtc gctatccacc ttccggtcgt tcgcccctgc
    18661 gtgaaaaaat gaacgggatt gccctgattc tgacaccaga ttcattcatc aaaaaagaat
    18721 acgttgattg atttttcttc atgccactcc agcgcggggt ggcattaaaa aaatcaaact
    18781 gcacaaacct ccctcctgta agtccggagt tccctcacct atgaacataa accgtttaat
    18841 cttcactatc gaagactgtc tgaacacgct atcccgtttc tctgtcgcaa gcagttttgt
    18901 tgagtattgc gatttacgta ctgtaattgg tctggacaga caggatcgtg aacgtcgccc
    18961 atggctgaat tcgccctata ttgcagccac aaagcgtgga gaatatctct ctgtctttga
    19021 agtcagcggt gctttcaggg aaatggatga agcatccgac cagactggcc caggttcact
    19081 ggaatcgctg atcaccagca tgagtgacag cctgaatacg gcatataaaa acagcggtca
    19141 taaaatcagc ttcgtttttg agcgtgatcc tgacatgggg aaagaagaaa tcgaagacat
    19201 ggtcgcccca caaaagcgct cactggctaa tacaggtatc cagttacagg atgtggtcga
    19261 tgagaaagtc acaacactgt ccccatggct ggtccgggag cgttgctggc tggcgatctg
    19321 gagtggaccc gaccttatca gcaacagcga tcgcaccgcc catgatgagc ttgtcagaag
    19381 actggctgaa cgggtaccga aagcacgctt tgcccagtcc ccctggcagt ggaccttatc
    19441 tgcactgaaa atccgccacg aagcttttct tgataatgtg gaacaggcac tgagacattc
    19501 atctgatggc ctgattctcc ggctactgga tatacacgag gtcgggcgtg aaatacgtcg
    19561 tcagacagaa cggcacagca caccgcgtaa ctggcaaccc cacctgcctg aagatgccca
    19621 gccagcggga tatcgctgga ctgacgatga atccgtacta catgctcctt cattgcattt
    19681 gcagttattc aacactcagg tgaccacaca gggcaacctg gttcaggcgg gtggactctg
    19741 gcacggaatg gtatcgatta ctttacctcc gcaaaatctg cagacgttta acgaactggt
    19801 ccgtgccgtc ccccgcgcag tcccctggcg catcagaatg gatctgatgc ccggcgggat
    19861 gaaggctctt aatctgaaga aaacgcttct gacttacagc agcttcattt ctgccgttcg
    19921 tccaatgtat gaatccgtta tgaccctggc ggcaacggat gaaaaagaac ctgtctgcat
    19981 aatgaccatc atggcctcca cgtggggaaa aacacgtgaa atatgcacac gaaaccaggc
    20041 cattctgaaa tcggccattg aaggctgggg cgtatgtggc accacgacaa catttggcga
    20101 tccccgacgc gcctgggtga ataccattct cgcggcatcc ggaggctccg gcccggttcc
    20161 cctgtatccc ccgttgtcac atgccatctc gcttttcccg cttaacaggg caggctctgt
    20221 ctggcgaggc aaagggaatc tgatgttaca tacagaagac ggttccgcat ttgaggttgg
    20281 tctggcgtca tcccaacaaa ataaacatac agaactggca ccaggcgatc cgggactggg
    20341 taaatcagtt ctgattaata cgttgtcgga aatacagata tcatctgctc agaaaaacct
    20401 gcccttcatt gcctatatcg ataagggata cagtgctcag ggactggttc agcttattcg
    20461 cgacagtctt cctccggaaa gaaaagacga agctgtcggt attattctga gcaatgatcc
    20521 ggaatacacc cgtaaccttt ttgacgtgat gtacggcgct aaaaagccta ttacgccgga
    20581 aaagaatttc atgtcatctg tgctgtgtgc tctctgtgtc gataccggta ccggtcaacc
    20641 ctgtaacccc ggagacaccc gacaaattat caaccagctt atcgagctgg cttttaaaga
    20701 gtacggtgaa aataatccgc gactttatcg cgcctcaacg gaagaactgg tggattcagc
    20761 actgcaggac tcgggactgt atgaaaaaca tgatgcaacc tggtgggcca gatcaacctg
    20821 gttcgaagtc cgggatatgc tccacaatgc cgggtatatc atggcggctc agcgggcaca
    20881 ttatcaggca atgccccagc ttcccgaagt gtcctcgatg ctgggccata ccagtctcag
    20941 ggatgttttc ggtactgttc agcgtgatgg ttcaaacgaa cttctgctgg actacatccg
    21001 ccgcgctctc gagcagggac ataacgacta ccccatgata tccgggtata cccggtttat
    21061 gatcaacccg gaaacccggg tcatcgctgt cgatctgaat aatgttgccg gtgacaaaac
    21121 gcctgcaggc aggcttaaaa ccggcatcat gtacctgctt gcaggtcaga tcgccggtgg
    21181 tgacttcacc ctgccacaat acagggatga agtgttaaag cagcttccac gggaatacca
    21241 tgaaatcgct cttaaacgta ttaaccaact tgatcaggag gttaagacaa aagtttatga
    21301 tgaactgcat aacgcccggg gcattgactt catctgggaa aatctggata cccaggagcg
    21361 tgagcaacgc aagtttgcta tccgcacggt tctgagtacg cagtatctga gggactaccc
    21421 ggaaagcgtt cttaagtcag caaatactct ctggttactg cgctacaaac ccgaagatat
    21481 ccccgtactg agggataact tcaacgttcc tgaatttatg ctgaaacgct tcctgaaaat
    21541 gccggaaggc ccggcaccag atggtagtgg tgttcctgtt ctgggagtat tcagggtaaa
    21601 aagtggcacg cttgcgagaa tactcaaatt taccgtcggt ccactggagc tatgggcgct
    21661 gaactcatcg ccaaaagaca gtgccctgcg taaaacactg acaaataagt taggttcagt
    21721 tcgggcacgt aagattctgg ccgaaaactt ccccagaggt tctgcaacgt cattaattga
    21781 gcaccgggcg ggccaacata acagtgacaa tgttattgaa gaactggcct cggagctcat
    21841 ccgaaaacag ggatacaatc tctgatgtgt acaattcata tcaccccacg ctgccggaga
    21901 cctgccagag ggcaggtatt tcttaaaaaa cttgaggtaa aaggtgacag tttctcattc
    21961 actctgccgt cacgtgtctt caggttacat ccggccccca atcccagagt gatatacgcc
    22021 tcggatgcca gaacacaggg aaaggtcaca ggcatgcggc tgttgttgcc gtccagcaca
    22081 gatgagctga taacaacggc aatatggcag gtttctgtcc cggatgaagc accattcgac
    22141 gttcagcatg tcatccagtg gatactccct tcaccgctgg cttcgctgat gtccgatgat
    22201 acctggttat ggccatccgt tcccggaacg ccggcactaa gtgccggtga ctggcctgtt
    22261 atggatactg acccttccac cttacggcgc gtcagacgtc gttctttcag catcatcgat
    22321 aaccggaaca ggaaaggtta tatcaccagg cgatatcagt gtttcccact ggccccacta
    22381 cctgaagaca gaaaatacga cagccttcct gaccttgtgc tccatcccga gcaggagaca
    22441 ataaaatgca aagaaaaaca ttactcgcag cccttatagc caccctgagt ggaacggcat
    22501 gccaggctca tgcttattca gtcaccgtcg tcgccagccg gcctgttgaa gagcaggtca
    22561 tccccagaat ggaagccatc aaagatgtcc ttggcaacat actgagtacc cagacagcca
    22621 cgggtaccgc tatcaatcag aactctgaaa agctggcaag tgtcattgcc cagaacggac
    22681 aggcgacacg tcagcagatg attttcagta atgaaactca gcgcctcgag gaggcacgga
    22741 aaagcttcac cgtgccagac tccatctgca gtgaatccgc atcagggatt gcaacagaaa
    22801 gcaaaagtgc ctcagcatcc gcagcctcaa aactcagtaa aggtggcggt gtgagcaacc
    22861 gtagcatccg cgaccgtctg gccagcgcag caaactctcc cgtgcgggaa gcttatgatg
    22921 gcgcggccat acacgccagt tactgtacag aagcagaata tgcacgtttt ggaggaaccg
    22981 ccgtttgtcc ttctgtgggc gaaataccag gtggagacag ccaggtacgc tcgatttacc
    23041 acggcgcagg tacagcggac acacctgcgg cactgacatg ggatcagaag caaatagatg
    23101 cagcgactgc ttacatgaaa aacacttccc gcccctctgc cggtcgtgct ctgggtaaag
    23161 gagaagtgaa tacccagagt gggcgaacct acgtcggact ccagaacgaa tacaacggca
    23221 tcattgattc tgcgtcgaat ccccaactga cgctgattgc tgacagcacg cctaatgaaa
    23281 gcacacgtaa agcactggct gaaacactgc agtcagactc agcggccgct tacttcgacc
    23341 aggtggcttc accagaagca aaagcacggg gatatatgag tacccgtgag tttgaagctt
    23401 ttgaggctgg ccggcgctac gccaacacag catacctggt ggatttacag gaaatgcagg
    23461 gggataacct gctccgtgaa ctggtaagaa tcactgcgca aatgaactgg cagctgaacg
    23521 atctgaaaga acagatccgc cagggtaatg ttatctctgg ccagcaactg gctctgactg
    23581 cccgccagta ttatgaaaaa caactcggta gccttgagaa aacaatcaat caggcaaacg
    23641 cacgttgaaa gctgacgtca gaacaggact gaaaaaatga ataagttacc ggaaaattca
    23701 gaccagacga aaaacgtcaa aaaatcacca tccataaccc gcatagccac ccgggggatt
    23761 attaacattt ttgttcctct gatggagact cgcctgataa cgaattctgt acagcatatg
    23821 ctggaacaac aaaaacgcag gctggacaga tttcgtcagg taaataataa aaaggcgcaa
    23881 ctccacctca cgtgggaaga agctctgcag gccagccata tgtcagtaga cgatctggat
    23941 cgtcgtttcc gccggcgcag aacagtatgg cgattctgtt gctggagtct gctggccatc
    24001 gctttgtttc tcagtggtat gctctttgcc gcctcttcat tacctcttac gactctggta
    24061 cgcgccatca gcactcttgt gttgatactg tcaggcgtcg ctctttgtgc ttccagagca
    24121 ctgattgtta cataccgcct ctggcagttg catgaacgaa aagtcagcga acccgagcag
    24181 gggactttcc gtgattttct gaacgaccgc aatggctggc gcaatgccac attaattgcc
    24241 gtaaccagta aacaatactg atcacgtcca gatgccagta ccactggcat gtcgccatcc
    24301 cccttaatta aatcctgtgt ggcctttgcc catacgcaat tctattccgg agttactgtg
    24361 aaaattttac ttcgcgcact ctgtgcaggc cttgctatat cctcactgcc ggcaatggct
    24421 tcagtgacct atcaggatat tgtgtctgcc gcaaccaatc ctgacgatct gtcccgtcag
    24481 gcgctggtca cgatattcgg tgatgtagtc accaatccac tgtcaaccag cgcccccact
    24541 ctgatcggaa gcatgttcgg ggcgttcaac agtatcatcg ccgtacttgc cgttgtctgg
    24601 tttatgttca ttggtatccg tcatgttgtg cgttcggggc atcagggaca ggtattcagc
    24661 accggacgcg atatcgtcgg cacactgagt gtcgttgcag gttttctgat gatcgttcca
    24721 accggaaatg gctggagtat cgctcagctc attatgttat ggggcgcatc gataatgggg
    24781 gttgggtccg ccaatgtgat ggtgcaactt gccgctgata atatcgcaaa tggttattca
    24841 atgactgtac agccagttca ggcatccaca cgaacagcag cgcgtggcat ttttgaaatg
    24901 gaactgtgta agtatgcggt taacgcagga ctaaatgatt ttaatcagac ggtcaaatcc
    24961 tcgacttcac tgatgacaga atcagctaaa accgccagtg gtaattacac tgtcacggta
    25021 tccaatggca gcggtatctg tggttcagcc agcctgtcag tagaaggaaa cggaactaca
    25081 gatcaatcga cgattggaaa attctttaat cccttcagta aaaacgaata cagcggtgtt
    25141 atctcagcac aacgtgcagc catggataat atgattagcg acatggacaa cgctgccagt
    25201 gagtttgtga caacatttct ggaaaaaaga aattctggca acggaacatt gccggatatc
    25261 gagacacgga ttcagagagc agccgacgag tatgaacgcg ctgtacaaaa atcgctacct
    25321 attgataacg gagaacaatc caggaaagaa gctctcaagt cttatctcac aacctatgga
    25381 tgggtgacgc ttggcgcgtg gtatcagacc tttgccactg ctaaccagcg tctggcagag
    25441 ctggctgaca gagctccagc tgtaacttca atgtcatcac ttggggaagt tggcgatact
    25501 gatttattta gtgctgttat gggggcatac aagactcaat tacagaactc cacatatact
    25561 ccaacgatag gaacgataac aactcaagat gaaagtaatg cagctaatag cacagaccca
    25621 caaagtgtgg tcatgaaaag tatagcgcca accatattaa aatggacaaa ccaagctgct
    25681 actgaatggt caggaacagg aacaaccagc aaccaagtta acccattaat taagatgaaa
    25741 aacatcggcg attatacatt gggtacaaca gaaattctct ggacaggata tacaactgtc
    25801 agagttctcg cgacaatggg tgataactct attttcggaa aggctgttaa tttgttctct
    25861 ggattaccta aaggatttgt tgctctgctg gatgctgcag ctcccccaat atatttcttg
    25921 ttgttcctgt tgttttgtgc gggtttttcc ttatcaattt acctaccatt tataccattc
    25981 attttctgga tgacaggcat tgggaactgg atagtaagcg tattaatagg atgcaccgcc
    26041 gggcctctgt gggcagcaac acatctagga acatcagagg acagaggaag tcgagcagct
    26101 tacggatata tatacttaat tgacagcatg ataagaccac ctctcatggt attcggtttc
    26161 ttttttgcct ctgtcgccat cattgccgtc ggcacaatat taaactcact gtttggcgca
    26221 gcactagtta acgtacaggt aaaatcatta acagggatat tcagccttgc cgggttcctg
    26281 ctaatttacg cccgtatgtg tacaacgtct gtagctgcaa tatttgcttt acaagcatat
    26341 cttcctgacc atgttatcaa tttcttgggt ggtagagatg gtgtaaatac tcttggcaac
    26401 ctcacctcat cagtaaaaga tatttttgca ggttctaata gaaatattag acacagccct
    26461 ggagttagag aggacagact aaaaaatgtc acaaaggatg ataataaaga tggaataaaa
    26521 ggataatttt aataaaacaa ccttgccata aacactcaat cgcattaaaa tatcgatgag
    26581 tgtttatgga gggtttatta tgcaaatggt aaaaacaatc aatagatatc ctgcttggtg
    26641 gacattatat tacgccctta gatttatata tttcataagc ataccagtgt tatccatttt
    26701 tattcttttc ggagtgctct ccattacatc ttcaagatat gtgacgcaag aagattatat
    26761 ttatacttgc gtctgtctgt ttttactaat tgcccctgca atattgatgt attcccgagc
    26821 aagctcaaga aaagacaaaa taaaaaaaat tgtcgcggag ataaaaaaca ccggttttta
    26881 ttcacctgac aaagaatatg agggactgtc ttttactcag ggcgtctatt ttggcgtgga
    26941 taccaaaaaa ggcactatgc tgtatgctcg tgcttaccct ggaaatataa tggatattat
    27001 cggctttgac attgacaact tcacacgcac tgtgaccgac gccaagacac tagagattta
    27061 tacaaaatac ataaatatac caatggtctc aattccgtca ggatgcatcc atcccaaaat
    27121 gatggcggat acaatgcatg caatggcaga aagaggatat gattaccctg tcgactttcc
    27181 tcgcctaatt caggagaagc gtaaggagtg ggagcagatt gcaggcatgc ctgtagccga
    27241 agtattttaa gataagctcc ctccataaca gccggttcaa atgagccggc ttttgttcat
    27301 tacaacagat aacttatgcc gttaccgata aattcgacca agtgctttaa tcgcagcaat
    27361 ttttatttcc atatcatcac tttgactcaa tctatgtaat tcacgagtaa tgttctccgc
    27421 ctgacaccgg ccttcaccta atgcatagat cgcagcaagt gtaacgtctt ttgatttact
    27481 cttcatcatt tcaaacaaga aagtattagc tatcgcagga ggcatgctca taatcaatcc
    27541 tcttcatgaa ataaacaatg gcactcactt acaaacagga gtccactatc gcaagcgact
    27601 caacttagca atatctctca atttgaatga aaaaaccaga tggcatgtga cagggaaccg
    27661 tccgcatgat gtccgtgacc atttaacccc ataagctaac cttgtcataa acgggcgcag
    27721 aacagcctgc cagacagaca acaaaacaaa agtccaagat aaaacatgaa taccggtatc
    27781 tcaatgcgaa gaaataatct ccgtagaaac ccccatgaga aaaatatcat atgggagaaa
    27841 atcgcaatta tcacagccag cgcattcatt attttcgctg tgctctggca ttaattattt
    27901 tcaacacaaa aaggaaaaaa caatgaatct ggtagacgcc tttgtcaaaa aagtaatatc
    27961 cgagccatat gaagaatatg gaaaatggtg gatagatgtt gaatacattt catggggtgt
    28021 tcccgggaaa acaagactga tgtttgagtc aaaggagcag gcactggaag ttaaagaagg
    28081 gtacaaattt ttaacgtgag atctcttttt gttaaagggc aggaagcggt aactccctgc
    28141 ccttagttac gtttccaggc ttatcagtcg acaagttccc ggatcagttc cagaacagca
    28201 atcagcgcgt ttgcaacggc aactactgcc ctgagaacga tgattaacag attaatcatc
    28261 cgcttctcca tcactggagg aaatcaccgt tgcttactct tacattgcct gtcacaacgg
    28321 cactttcagt gttagttgaa aagagacttc ctcctgccag agcactgaac acagcaccac
    28381 ctgtttgttc agccaggact taaaatcttg catccatagt cccgatggat atcacggcag
    28441 aagtgtcgcc agacagagct aacctgcgga ttataaggat ggttgctatc aaatgaacag
    28501 ctcgctatta taagaacgtt agttgaaaag agatttcctc cggtcagggc cacccactct
    28561 gtaacggaac agtaaaaaaa ccgacctcac cagtcggttt ttttacatct gttgtttctc
    28621 aaaatccgcc cgacatgccc gtcacaccgg ggaaactcat gctctgaatc agaaccaatc
    28681 atctcaactt tgccgtctcc tgaaaaaact ggcacattca gttcgataac tacccataaa
    28741 tacagagaga aaatccgatg tcagaaaagt ggagtaaaga attcgactct tgctttcagg
    28801 tggggagcat agaagaaatt gccgatgctc tcgagaaacg cagcggaagc gaggaaaacg
    28861 cgttagccat gctggcaatg acagccttca cactcatgac ccgacgtggt atctgcgaaa
    28921 tgcagaacgt ggcccccgat ggcaaaggca ttcggctgga gctaatcggc ttcaggggaa
    28981 atgaaacaat accggatacg cttcattgaa gaagaaaggg cgcaatgagg tcagccttcg
    29041 caacaaagcg gcggcagaaa acctgccgcc gtgttgcccg ttaacgttta acttcgtagg
    29101 ctaacgttgc cacaagcact gtgtttccct gctggagccg gagtacgcaa agcgaatccc
    29161 tcaccatcca gacaaaacac agaatcgtca cacagatgac gattaacatc attaaaaacg
    29221 ttcgctgtgg catcatagcc cctccattgc cttacggcgg ttatcgggct actataatgt
    29281 tgtctaggca ttacagtaga gcccttggtt gattgataaa aaaatctcca ggggctttct
    29341 gctgtatacc gtccacaaaa cgctttcggc atacagcctc aagcgcccgc agctattcta
    29401 acgactttcg tatctgcaac aatcctttta tcgcacggct aattcctctg gttagctctt
    29461 atcgcctgca aataaatttt taacctttcc gtttgattta aattccagaa atggagactt
    29521 tgtcatgtct tacaatcgac agcctgtcgc agaagatcct atgcagatat ggggcgctgt
    29581 tggtgtcctt cttatcctgc tgctattcgt catatggtta ttcttgccgg aagtggttta
    29641 cgccagctgc ctgattcttc ataccttatg gggactggtc gactggggac catttcataa
    29701 ctatgcagca ccacgataca acctgcttgc catgaccggt aacaacgccg ccaacataag
    29761 ctatagccag tgggttaacg tcatggagca gaccataggt attctgtgga tgtacctctt
    29821 acctgtgaca ctctggtgtt tgtgggaatg gtatcaacat ccgggacaaa gccgtttcac
    29881 ccgcagacca gtagatatta cccgactgcc acacatcttt gcttcgttgt cccctgctat
    29941 cgcccctgta ctggcagatg gtgatccgga aaaactcttt catggtggca aacgacctga
    30001 acggcgggtt gctctcaccc cggaagcatt tgtcgagcag catactctca tcaccaatat
    30061 gcagctcgat gttgcagctg caagacgatg tttcatggcc cagttgggta aacctttaac
    30121 gtcctggaaa gacatggcac cacatgaaaa agcgctcttt gccatatttg gtcttcagta
    30181 tttcctggat gatcgtaagg cagcattaaa gctcatggat acactgaacc tctcatgcag
    30241 aattaaaagc aaacgggaca gtgggaaatt ctgtacccct gtttattcac tggccaaatc
    30301 agctttccag agagtgatta aaagcaacgg agcacagcaa tggctgaaac aacatcgcta
    30361 cgtccgcagt gggctggtct ggctgtatgc acatgacctg cggcttacgc cacccaactg
    30421 gatttggctg aaaggcgtgg acaggacgct gttttatgca ttacaccgcg ccaataccac
    30481 gaaaggattt attgaaggcg ctggcgtggt ggccgttgca cgtgcagagg ccgaagccat
    30541 gcgttttggt cttccctgcc ctgaaccctg tgttgatgaa gcggtggaag gcttacggcg
    30601 ggatatgctc agtctcggtc ttatctggga tgaaccacaa ccagatcgcg accggaaacg
    30661 ccgcatcctg accaactggt ccctcaccga cgacatcctg ccccgcacac ctgctacaga
    30721 caacgaattc tgattatccg gagaactgaa aatgacaatc gagtactttg caacacgcat
    30781 tgcaaaacat atttccgtgc aggcaatctg gccggatggc aggacagaaa ttattgccgt
    30841 ccttccacgg gatgccctgt cagggctggt aacacgaaat aatcaactga ttcatatagc
    30901 ctttgacgcc atgggcaccc gtaacagaga aaccgtcctg attgataaac aggagcataa
    30961 cgctgaccag atacttacag ctattgattc ctgcctgcgc cttgaatatc tgatggaacg
    31021 ccgcttcagc agaacacctt tgttccgtgg cataatcgcc agcgttgtgc tgttcgtaat
    31081 ggcaacgatt ggcttctctc tgttccgcta tgttgatcgt gtattctggg atgatacaac
    31141 gcctgaagca gtacagacag caggtgagcc acgactgcta ccccctcacc tgaatcacac
    31201 tgtgccatta aatgaaggca ttcaactccc ggttcccccg aaaaaagacg tccaggtccc
    31261 ggagaaaaca atcacaagca aaaatccgga agccgcagct gcgcgacata atctggctac
    31321 tgttctgaag cggaacgccg atcggggcat gtttaccatc aacctgtcat ccggtcatga
    31381 acgaaccctg ttcgcatttc tcgatcctgc ctgtcctaac tgcagacttc tggagccggc
    31441 cttaaaacgc ctggccagcg acttcaatgt cgttatctac ccggtttcag ttattggtgg
    31501 agaggaatcc accgaccgtg tggcaccact gctgtgtgaa aaagatgctc agaaacgtgc
    31561 tgccggctgg caccgactgt attccgcaga taacggaatg atgacgccac cagaagaaac
    31621 aacgcctgca gatgaaacat gcctgaaagc ggccagagct gcaattgatg taaacaatgt
    31681 cgccttccgt aagttcggat ttgccggtac cccatgggtg ctgtccgaca ccggctggca
    31741 tctgcctaca ggaattctgc aggaaactgg cacgcttaat ttattcctga aaaccaccga
    31801 cagcgagtct ggccatgagt gaacatcgcg ttaaccccga gcttttacac cgtacagcct
    31861 ggggaaatcc tgtctggaat gcactccaga gtctcaatat ctatggcttt tgcctggtgg
    31921 caagtcttgt ggccagtttt atctggccac ttgcattacc ggcatgtctg ttgttcacac
    31981 tgataacaat gctggtattt agtctgcagc gatggcgctg ccctcttcgt atgcccatga
    32041 cgctggaatg tgctgacccg tcacaggaca gaatgattaa aagaagtctt ttcagcttct
    32101 ggccaaccct gttccagtat gaggtcattc tggaatcacc ggccagcggc atattctatg
    32161 ttggttatca acgcgtcagg gatattggca gggagttatg gctgagtatg gacgatctga
    32221 cccgccatat aatgtttttc gccaccaccg gtggcggtaa aacggaaacc atctttgcct
    32281 gggcgattaa cccactttgc tgggcaagag gattcaccct tgtcgatggc aaggcacaga
    32341 atgatacggc aagaacgatc tggtatctgg ccagacgctt tggacgtgaa gacgatgtgg
    32401 aagtcattaa cttcatgaac ggaggaaaat cccgcagtga aatcattctg agtggtgaaa
    32461 aaacgcgtcc tcagtcaaat acctggaatc ccttctgtta cagcactgag gcattcacag
    32521 cggagacaat gcagtcaatg ctgccccaga acgtccaggg gggcgagtgg cagtcacggg
    32581 ccattgccat gaataaggca ctggtatttg gtactaaatt ctggtgtgtg cgcgaaggca
    32641 aaacaatgtc gctgcagatg ctccgtgaac atatgactct cgaagggatg gccagacttt
    32701 actgtcgtgg cctcgatgat cagtggccgg aagaagctat cgcaccgtta cgtaactacc
    32761 ttcaggacgt tcccggtttc gacctgtcgc tggtcaggac accatcagcc tggacggaag
    32821 aacccagaaa acaacatgct tatctctccg ggcagttttc tgaaactttc agcacattca
    32881 cggaagcatt cggggatatt tttgctgaag attccggtga tatcgatatc cgtgacagca
    32941 ttcacagcga caggattctg atggtaatga tccccgctct ggatacatca gcgcatacaa
    33001 cgtctgctct tggacgaatg tttatcaccc agaaaagtat gattctcgcc cgtgatttgg
    33061 gctaccgcct cgaaggaact gattccgatg cactggaagt caaaaaatat aaggggcgtt
    33121 tcccttatct gtgtttcctt gatgaagtgg gggcatacta tacggaccgt attgctgtgg
    33181 aagccactca ggtgcgttca ctggattttg ctctgatttt gatggcacag gaccaggaac
    33241 gtatagaagg acagacaaca gccacgaata ctgcaacact gatgcagaac acggggacaa
    33301 aatttgccgg caggattgtc agcgagggca gtacggccag aacgctgaaa agcgccgccg
    33361 gtgaagaagc cagggcgaga atgaacaacc tgcagcgtca ggacggcata tttggtgaat
    33421 catggattga cagcccacaa atcagcatcc tgatggaaag caagataaat gttcaggagt
    33481 tgattgaact tcatcctggc gaattctttt ctattttccg gggagaaacc gtaccatcag
    33541 catcattctt catccctgac gatgagaaaa gctgcagcag cgatcccgtt gttataaaca
    33601 ggtatatcag cgtggatgcg ccacgcctgg accgtctccg ccgtcttgtt ccccgtacca
    33661 ctcaacgtcg aataccgtca ccggaaaatg tcagtgccat cattggcgtc ctgactgcaa
    33721 aaccctccag aaaaagacga aaaattcgca cagagccgca cacgatcgtg gatacattcc
    33781 agcaacgcat tgcaggacgt caggcggcta tggcaatgct ggaagagtac gacacagata
    33841 tcaacgcacg tgaaagtgcc ttgtgggaaa cagcggtgaa tacgcttaaa acgaccacac
    33901 gggaagagcg acgaattcgc tatatcacgc tgaacagacc agaactacct gaaacaaaag
    33961 aggaaaatca gatatcagtg agagcagaaa gagcaggtat aaacctgtta acgcttccac
    34021 aggacaataa ccacccgaca gggcgtcctg taaacgggtt tcatcataaa aaaaccaacc
    34081 gtccggactg ggacggaatg tactgaacag tggccgcgcc ggattctggc gcggcctttt
    34141 ttttaacctg gagaaggcgg ttcccagcgt ttatcctgct ccggcggagg cagtgatgcg
    34201 tcatcgttgc tgttcgccgc gatataatcg cggtagtagc gttcacttcc ttcacggtga
    34261 tgatccacgt ccggtcgcgt aatcctgtag tcttcgtgac cggtacggcc tgtcacatta
    34321 tgccaggcaa ccatttcagc aaagacctgg ttaaattgtt tgtcgtttcc ttctggttca
    34381 aaacccatgc gtggatcacg cccgaataat accggtgcaa tcaaatccag tttatctgcc
    34441 agcgcattgc ggtcatggtg attacggaaa acaaccctgt caccataatc cgtacagaca
    34501 aactctcccg tccgacggtc acggaaacga acactcccgt tcttctgcag acgggcctcg
    34561 agatcaccgg tattaccgta taccggcgtt ccgcccggtt cacaaagcac gacacagcgg
    34621 tctgtcgtcg ttgtgcgtga cctgtcttta cggcgatcgc gataatccca gcctctcagc
    34681 tgcgatacag cagccctgtc tccctgagca gcctgaattt cgacccactg acggtaagag
    34741 ggtggatacc atttcccatc agcaataagt ttctgtcgct catccctgat gtcttctttc
    34801 agcctgatca atgcctgcat tcgctgaact tctgcaatgt gataatgcag cttacgtaat
    34861 gccggatcat cgtactgcgc acgaatgtgt gacttccgca gacggcatgc ctgatgaatt
    34921 tcccgacaac gttccccata acgcagatct ggtttacgcc attgttcacg ccatgccaga
    34981 tagcgggcac gcaggtcagc acgggcctca gctctggctt cacgtcgctg ccggcgcagc
    35041 attgggtcac gtttactgct gaccccgtac ctgtcactga cagccagctc cgggttatag
    35101 cgactttcag gttgcaccct gtcaaacaaa tctgctgggg cactcacaaa aggtccggcc
    35161 tgtggctctg cacgccccag tgtcagatca ggatgaatgc tgctggcctt caccggcgtt
    35221 tgctcatgat taaaggcatc aacgacaaca agcccgtgat gttgtttctg tagcatcagt
    35281 ccctgttgtg caaacagctc atgacaacgc tgccaggtaa actcaccctg tgtgatcatt
    35341 tcttcaatct gagggctaca atggctgatg ctgtatcccc acagactttc tttgtcagaa
    35401 aacagttcgc gtttgatagc cccctgcggc cccgtgcctg tacgcatacg gtgccattct
    35461 ttatcggtcg caggacagct ctccatacga cgaccacgga agcgttctgt tggcgtaacc
    35521 tggcggaaaa tatctgttgg tacaggctgc cagccaccat cgagttgatt cacattgtcg
    35581 agcgtcagca gcgaccatac gctgtcagca cgaacgggag tctggttatg atcataaccg
    35641 tcacagataa ccaggtgacc atgctgaaca cgcatccata atcccgcttc agccagtgtg
    35701 cggtgaatag cctggcagtt ttccagccgt ccttcccgtg ccatttccgg cagacgttct
    35761 ccaagatgac gacaggcata ctgctgcaga ctttctgttt cagcgatttt ttccgggcga
    35821 acatcagcgg caatgaagtc cggattatag cgtcctggtg cttccacctg gctgaaaatg
    35881 tcttttggta ctggcgtgta gtcacccatt tttttcatga gtttttctgc acaccaggag
    35941 ggaccaaacg agtcaagctg tacaccttcc ctgttgcgat cccagccatc catcaccaga
    36001 aattttccgt ccatctgaga caggtacaga ccatcttccg caagacgacg atgcagggat
    36061 aaccaatcat ttacaggttc actgcgtaaa ccagcgaccg ctgtctgcgc gacatactcg
    36121 cgaaaagttt gtttttttcc gcgtgtccaa gcgttctggc gatcacgttc aacggcagtt
    36181 ttgcgaacga tacgatttcc cggcgcatgg acccagcaac cgttatccgg agcaaaacca
    36241 tgcttcagtt caagctcacg gcaggcacgg ctgagttttt cctgactcca tgaaaggcaa
    36301 ttgagataac ctgtaaccgg atgtacccgg ttgaccgcaa cgtgaacatg caggttatca
    36361 gtatcggtat gtacggcaga aacatactga tgttcaccca gcccgaggga tttcagggta
    36421 tggcgtacac tgtcataaat ctgttccgga cgcgggcttt catgggcctg ccaggaaaga
    36481 atgtagtgaa aaacaggatc agtattatct ttcgcatagt gtgcctgctg tgcaatgtac
    36541 tccatatctg cagcggcagt ttcaagagat gtacagttgt gaaaacaggt cacaccataa
    36601 aagttaaccc attcgcagcc gtccttcatg acatcaacca gcgccacgaa tgactcattt
    36661 cgaagacgtg tcgcataatc aacaaggcga ctgaaacggc ttcggtgcgg ttgttcagcc
    36721 tgcgaagaag aagataaatc cagttcctca tcggtcatgt catcccggac agagacgtag
    36781 gataccaggt cttcgaacga agacttgccg tcccttctct ttttcggaat gactgcattc
    36841 accggagaat cctcaacctt ccattaactg tttacgcaat gtatttgtaa gttcggttat
    36901 ggcaacaagt acttctgcgt attccttgtc accggttctt tttccctgaa caaacagatg
    36961 cttctgcatc cttcccagtc tcattaactc cttcagaaaa gcatcatctg tacgggagtt
    37021 aattcgctta ttcagtgctg catttcggat gtaagcagat acagtaagtc cggaatcttc
    37081 agcttttttc ctgatggttt catcctcaac aggagagaat ctgagtgtcc tgacaactgt
    37141 cttttgccgt acctcacttt tctttcttac cgcactatca ctcacttcag gctccttacg
    37201 gggtgtcggg gcgaagccct gaccagatgg taattgtaat agcgtcgcgt gtgacggtat
    37261 tacaattaca catcctgtcc cgtttttcag gccattatag cctgttattt ttgtgcatca
    37321 tagtcttatg cagacggcag aggcagggcg cagccctgcc aggagtcagt ttttgatgat
    37381 aaaatcagac ttcaacatga aacggagaga aagtgccaat ggcttctaaa aaattttact
    37441 cagatgatga catccagctg gcaaaagctg ctttatcgga attgccagat ctcaccgctc
    37501 aacggaaaac attgcgtgat tttcttgatg ccattcgtga tgacattatt gtcctggtaa
    37561 ggacaaaggg ttatacactg gcagatgttc gtgacacgct caaaaatgca gggtatgaag
    37621 taggagagaa ggcacttcgt gacattattc gggaagcaga atcgaaaaaa ccatcccgcc
    37681 gttcttcctc taaaacagca cctaaaaaga cagattcagg caggaaagat ggcatcgata
    37741 tgaacaacaa ttgatgtgac aatgtcacat cgtgttctga gattatgatg attacagaaa
    37801 aaaaagcccc ggcataaata ccagggcttt tctgtttaat cagactcgtg gcggcaatgc
    37861 acctggataa tgccgtgcaa taatatcatt cagtttttca acaaggtcag aacgcttaaa
    37921 gacaatgtgt acggtccctt tctggtagcc acgcaccgta aaatattccc cttctaacag
    37981 cttaccgaca ttgtcgccgt tcccgagaaa ttcaccatac tgcgccccga tactgttcct
    38041 gaagtcagga acgttgcgcc cctccagcag ataaaacgga cgtaccaggt catccagtgc
    38101 ctcccgtccg ctgcagtcca gcgtcacacg cccgttactc cagcggtaca gcaggttttc
    38161 aataatgatt ttcttaccca gacggcaggg attattggtt ctgtaatccc atgacaattt
    38221 ccggtagaca tcaatcagtc cctgttcaaa tgtctgcatc ttgcaggcat tcagatggcg
    38281 gaatgttgcc agtacattgt ccagggtgat ttcaggacag gtgtcgctca tcagctggct
    38341 gttccactca tcacgctggc atgaactcat gaaggtgtac atgcccgtat ctgtcatcag
    38401 gcggtcccag attttccggt cggcaagacg ggtgatattc cttttcgctt cttccggttt
    38461 atctatgcga cgaacggaat cagtcacgca gtcttcaaac ccgtaaagaa aaatatcacc
    38521 tgacgcatcg agcagaatct tgcgcgctgt cgccaggcat tccatcgcct gcatgaacag
    38581 tgcaatacct tcgttacgct gcgcgatgat gcggtcgata gccacagacg gaataacatc
    38641 tgtcgtgccg gcagtattca ggtcagcagt gggtaaagtc tgattcatca cattgtctcc
    38701 tcatcgtcac gactgccggg acagccaggt catttcccgg aggagcgaag ctccttctgc
    38761 cgtccgggtc ttcggggatt tccccgcagt ggacctttcc gacgggggac gctcaccacg
    38821 tcacggggca gttgtccggg ggcagacgca aaagtttttg acgcctgcgg caaaaagttt
    38881 tgtggctggt acatctctcc cctggacgac tgaaacggga tgtggtcatg tatgtacccc
    38941 cggaaagtcc gtctgcggag aacaggccgc agtgcggcgg cagacaggca gagacagctg
    39001 cgccatcagg cgcagtggcc ttacacaccg taaggtgtgc agggagcgcc gcatacggcg
    39061 cgactgaaga aaaacgacgg cacaacaatg gcatgtggtg gtcactggca caccactccc
    39121 gcacaggtga aaacagagaa aaccgcacga catttacaag caaaaaaagt gaaatttctt
    39181 cactttttta cacttattta tcgcgctgat ttggcaattg tgatgataaa ataaagtgaa
    39241 gttttttcac tttttcaaag gtgttttatg accggctggg aactgcgaat ctggcgaaaa
    39301 agcatgctct ggtcacgcga aaaagcggcc agagaatttg gcgtgacgca gagaacatgg
    39361 cacgcctggg aaaatgcgga acaggtggac gtcaccgtct ggaggaccac ccaggcactt
    39421 tcagtccgtg acctgctgcc tcacatgcag gggatgcgta aggcagatat catcaggcga
    39481 ctggaaaatg aactggggga aacggcagag gatgtgtgag cgtgattcac agcctggctg
    39541 aatgccaggc tactgctcat caggaaacag ctttttcagt tcttcaccgg aaagtccggt
    39601 tgcctcctga atccgttccg gcgtccagcc catatcccgc agccggcagg ctatttcccg
    39661 cgccacttca agtgcgcctt tttgaataag acgttctgca atcgtcatgc tgtttgctct
    39721 gctcctgctg acaaaaaagc ccggcatttt gccgggctgt gatgtttact cagtgagtga
    39781 ttttcttcag ttcgtcatcg gaaagtccgg tagcctgctt aatctgctcc ggcgtcatcc
    39841 ccattttctg aagctggcac gcaatagcca gggctttttc aagcgctcct tccatgcgtc
    39901 cttccatcct gccttcctgg cgaccttttt gctcaagacg ttctgcgata gtcatcagtt
    39961 tatcctcatg ctgcggcaga cgctcagcca gcgcccgcat aaattccggt gatacagagt
    40021 cccctgcgtt aacgatgtaa tgaatcagaa cacgcacctg ctcgtctgaa atttccacca
    40081 tgacctgcgg gagtttgtcc agcagctcca tcatgtcacg atggcggata tgcttcatca
    40141 gcagcgtcag tgcggccatc cggcgatggt tcatgatttc attgtcatca acgacagtga
    40201 tatcaaccag cggaaacggc tttgtgtata ttttagccgc aagcgccgga ttctcaaaac
    40261 agtccagcca gttcatgctg tacgggtacg ggctgcgctc accgtgatag aacagcaccg
    40321 gaatgaccag tggcaacgtt ttgtgctgct ccagatgtct ctgcatcgcc gccacactgt
    40381 aacgtaacat gcggaaaggc atgtacagat tttcggtgct ctggtgctcg atgagcgtat
    40441 aaacatagcc atcttcaccc ccggtggttt tcacactcca gaggatatcg ctggcgtact
    40501 gatgcaggtc cggctcaaca aaggtggcgg gttccagctt cagcgtggac aggtcgcaca
    40561 actgccggta ctccgccgga agatgcagtt ccagaaaatc tctggcgacg tcgggattcg
    40621 ccaggaacga ccggaacgcg gcatcatgcg gcgtgggcgt cgtggtggtt ttcttcttac
    40681 tcacggttcc tcctggtgag atgtctgaac gtatcctgcc gcctgcacag gcagacggca
    40741 agggttaatc agtggcagac ccgctcaatt tcaagcgcgc ggagcaggtg gccccattcg
    40801 ctgactttca tagcggggat acgcacccgc ttctgctcac tgcagcgtct ggcgatatgg
    40861 ccgctcatcg ccaggacgtc cgggttaatc tcatcctgtg ccagctcctg acgtctgaag
    40921 cgggtatggc ggtgcagggg tttattctcc cgcaggcact cttcacgcag acgctttaca
    40981 tactcggcgg ttgcgaagat aattgatggt ctcatggcat aatctccata gatagtgatt
    41041 gagatgccgg acttcctggc ggggaagcgc cggcggagag aggcagggtt tatcgccctg
    41101 cctttttttc atttcggaca gcgacgcagt gccagcgcga gcgctttcat cttccagcct
    41161 tccggcgtgc ggtacatcac cttccagtag ccgtcgatga acacataatg gaagcgcacc
    41221 tcttcagtgc tttcagtggc aatagcctcc ctgaaagaac ggtactcacg cgcctcactg
    41281 tccatccgga aaccactgtc acgtccgtaa taagccgtca caccgtccac cgggttctga
    41341 agcgtatgcc cggcgggttt gtcgcaacgg ggaccgaggc aacggatatc accgtgttca
    41401 accagggctt cggcctgctg accggtggca tagtgggtat gcaggatgcg accgttccag
    41461 gtcggctccc cgtgcaggtg gcagtaaacg gtacggaaca ccccgtcgcg gcaaaaaacg
    41521 gaaatcgtgc tgctgactgt catgaaaacg tctccttctg ctgaatcagg tcgccgggcc
    41581 gaccggttgc tccccggagg ggcgaaagcc ccttctgccg tccacttctt cggggtttct
    41641 ccccgcagtg gacctttccg atggggcgtc cgtcaccacg tcacggggca gctgtccggg
    41701 ggcggacgca aaattttttg ccgggtttat ggctaaaaat tttgtggacg gtatatcttc
    41761 ccctggactg ctgaaacggg atgtggttac tccagccccc ggaaagtcca cagcgggaaa
    41821 caggccgcag gggtggcggc ggaagggttg actgcgccat caggcgcagt ggccttacac
    41881 accgtcaggt gtgcagggag cgccgtttcc ggcgcgacag gggcggcgcg accggaacgg
    41941 tcgctgcaag cctggttttt cag
//
LOCUS       DAZFTN010000029        41689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFTN010000029 DAZFTN010000000
VERSION     DAZFTN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..692
                     /gene="yqiJ"
                     /locus_tag="ACHKV2_001734"
     CDS             78..692
                     /gene="yqiJ"
                     /locus_tag="ACHKV2_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6939634.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            734..2395
                     /gene="yqiK"
                     /locus_tag="ACHKV2_001735"
     CDS             734..2395
                     /gene="yqiK"
                     /locus_tag="ACHKV2_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6939635.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            2634..2693
                     /locus_tag="ACHKV2_001736"
     CDS             2634..2693
                     /locus_tag="ACHKV2_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6939636.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3010..3069
                     /gene="ibsE"
                     /locus_tag="ACHKV2_001737"
     CDS             3010..3069
                     /gene="ibsE"
                     /locus_tag="ACHKV2_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO6939637.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(3189..4622)
                     /gene="hldE"
                     /locus_tag="ACHKV2_001738"
     CDS             complement(3189..4622)
                     /gene="hldE"
                     /locus_tag="ACHKV2_001738"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO6939638.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4670..7510)
                     /gene="glnE"
                     /locus_tag="ACHKV2_001739"
     CDS             complement(4670..7510)
                     /gene="glnE"
                     /locus_tag="ACHKV2_001739"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO6939639.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(7533..8834)
                     /gene="ygiF"
                     /locus_tag="ACHKV2_001740"
     CDS             complement(7533..8834)
                     /gene="ygiF"
                     /locus_tag="ACHKV2_001740"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO6939640.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9076..9696
                     /gene="ygiM"
                     /locus_tag="ACHKV2_001741"
     CDS             9076..9696
                     /gene="ygiM"
                     /locus_tag="ACHKV2_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO6939641.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9760..10998
                     /gene="cca"
                     /locus_tag="ACHKV2_001742"
     CDS             9760..10998
                     /gene="cca"
                     /locus_tag="ACHKV2_001742"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO6939642.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(11179..12000)
                     /gene="bacA"
                     /locus_tag="ACHKV2_001743"
     CDS             complement(11179..12000)
                     /gene="bacA"
                     /locus_tag="ACHKV2_001743"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6939643.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12091..12459)
                     /gene="folB"
                     /locus_tag="ACHKV2_001744"
     CDS             complement(12091..12459)
                     /gene="folB"
                     /locus_tag="ACHKV2_001744"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO6939644.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12565..13182
                     /gene="plsY"
                     /locus_tag="ACHKV2_001745"
     CDS             12565..13182
                     /gene="plsY"
                     /locus_tag="ACHKV2_001745"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO6939645.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13195..14127)
                     /gene="ttdR"
                     /locus_tag="ACHKV2_001746"
                     /gene_synonym="dan"
     CDS             complement(13195..14127)
                     /gene="ttdR"
                     /locus_tag="ACHKV2_001746"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO6939646.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14334..15245
                     /gene="ttdA"
                     /locus_tag="ACHKV2_001747"
     CDS             14334..15245
                     /gene="ttdA"
                     /locus_tag="ACHKV2_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO6939647.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLTRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15242..15847
                     /gene="ttdB"
                     /locus_tag="ACHKV2_001748"
     CDS             15242..15847
                     /gene="ttdB"
                     /locus_tag="ACHKV2_001748"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO6939648.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15896..17359
                     /gene="ttdT"
                     /locus_tag="ACHKV2_001749"
     CDS             15896..17359
                     /gene="ttdT"
                     /locus_tag="ACHKV2_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO6939649.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17402..18415)
                     /gene="tsaD"
                     /locus_tag="ACHKV2_001750"
     CDS             complement(17402..18415)
                     /gene="tsaD"
                     /locus_tag="ACHKV2_001750"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO6939650.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18653..18868
                     /gene="rpsU"
                     /locus_tag="ACHKV2_001751"
     CDS             18653..18868
                     /gene="rpsU"
                     /locus_tag="ACHKV2_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO6939651.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18979..20724
                     /gene="dnaG"
                     /locus_tag="ACHKV2_001752"
     CDS             18979..20724
                     /gene="dnaG"
                     /locus_tag="ACHKV2_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO6939652.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20919..22760
                     /gene="rpoD"
                     /locus_tag="ACHKV2_001753"
     CDS             20919..22760
                     /gene="rpoD"
                     /locus_tag="ACHKV2_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO6939653.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22839..23345)
                     /gene="mug"
                     /locus_tag="ACHKV2_001754"
     CDS             complement(22839..23345)
                     /gene="mug"
                     /locus_tag="ACHKV2_001754"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO6939654.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            23470..23545
                     /locus_tag="ACHKV2_001755"
     tRNA            23470..23545
                     /locus_tag="ACHKV2_001755"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23503..23505,aa:Ile,seq:cat)
     gene            complement(23599..24363)
                     /gene="nfeF"
                     /locus_tag="ACHKV2_001756"
                     /gene_synonym="yqjH"
     CDS             complement(23599..24363)
                     /gene="nfeF"
                     /locus_tag="ACHKV2_001756"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO6939655.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            24651..25274
                     /gene="nfeR"
                     /locus_tag="ACHKV2_001757"
                     /gene_synonym="yqjI"
     CDS             24651..25274
                     /gene="nfeR"
                     /locus_tag="ACHKV2_001757"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO6939656.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(25428..26948)
                     /gene="aer"
                     /locus_tag="ACHKV2_001758"
     CDS             complement(25428..26948)
                     /gene="aer"
                     /locus_tag="ACHKV2_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO6939657.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            27366..28745
                     /gene="ygjG"
                     /locus_tag="ACHKV2_001759"
     CDS             27366..28745
                     /gene="ygjG"
                     /locus_tag="ACHKV2_001759"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO6939658.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(28787..29119)
                     /gene="ygjH"
                     /locus_tag="ACHKV2_001760"
     CDS             complement(28787..29119)
                     /gene="ygjH"
                     /locus_tag="ACHKV2_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO6939659.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            29338..30321
                     /gene="ebgR"
                     /locus_tag="ACHKV2_001761"
     CDS             29338..30321
                     /gene="ebgR"
                     /locus_tag="ACHKV2_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO6939660.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30505..33597
                     /gene="ebgA"
                     /locus_tag="ACHKV2_001762"
     CDS             30505..33597
                     /gene="ebgA"
                     /locus_tag="ACHKV2_001762"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO6939661.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            33594..34043
                     /gene="ebgC"
                     /locus_tag="ACHKV2_001763"
     CDS             33594..34043
                     /gene="ebgC"
                     /locus_tag="ACHKV2_001763"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO6939662.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            34106..35539
                     /gene="ygjI"
                     /locus_tag="ACHKV2_001764"
     CDS             34106..35539
                     /gene="ygjI"
                     /locus_tag="ACHKV2_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6939663.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            35673..36743
                     /gene="ygjJ"
                     /locus_tag="ACHKV2_001765"
     CDS             35673..36743
                     /gene="ygjJ"
                     /locus_tag="ACHKV2_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO6939664.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            36760..39111
                     /gene="ygjK"
                     /locus_tag="ACHKV2_001766"
     CDS             36760..39111
                     /gene="ygjK"
                     /locus_tag="ACHKV2_001766"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO6939665.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            39637..41655
                     /gene="fadH"
                     /locus_tag="ACHKV2_001767"
     CDS             39637..41655
                     /gene="fadH"
                     /locus_tag="ACHKV2_001767"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO6939666.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT        10053 a        11294 c        10767 g         9575 t
ORIGIN      
        1 attaacaaag acaaatgtaa gcaaggaaac tatcacaaag taaatattgc gtcactaaat
       61 ggacattgga gtgacatatg attttattcg ccgactataa caccccttat cttttcgcta
      121 tttcctttgt actgttgatc ggccttttag aaatttttgc gcttatctgc ggtcacatgc
      181 tctccggtgc tcttgatgca catcttgacc attatgattc cataaccaca ggtcatatta
      241 gccaggcact tcattatctc aatatcggaa gattgccagc tctcgtcgtt ctttgcttac
      301 tggctggttt ttttggtctt attggcatcc tgttgcagca tacctgcatc atggtctggc
      361 aatcaccact ctcgaacctg tttgtggttc ctgtcagttt actatttacg attattgcgg
      421 tgcattatac cggtaaaata gttgcgccct ggattccacg cgatcatagc tcagctatta
      481 cagaagaaga atatattggt agcatggcgt taattaccgg tcatcaagcc acttcaggta
      541 atccctgcga aggaaaactc accgatcatt ttggccaaat acattattta ctactggagc
      601 ctgaagaagg aaaaattttc actaaaggag acaaagtgtt aattatttgc cgactttctg
      661 caacgcgata tttggcggaa aacaatccct agccccaaat attgtaattt attaatcaaa
      721 aggaaattta ataatggatg atattgttaa ttccgtgccc tcctggatgt ttaccgcgat
      781 tattgccgta tgcattctgt ttattattgg aattattttc gccaggctct atcgtcgcgc
      841 ttcggcagag caagcatttg tccgtaccgg cttagggggg caaaaagtgg taatgagcgg
      901 tggcgcaatc gtgatgccga tctttcatga aataatcccc atcaatatga atactctgaa
      961 gctggaagtc agccgctcaa ccattgatag cctgattacg aaagatcgta tgcgcgtcga
     1021 tgtagtagtc gctttctttg tgcgggtaaa accttcagta gaagggattg ccaccgctgc
     1081 ccagacgctg gggcaacgca ccctgtcgcc tgaagactta cgtatgttgg ttgaagataa
     1141 atttgtcgat gccctccgtg caacagctgc gcaaatgacc atgcatgagt tacaggatac
     1201 ccgcgagaac ttcgtgcagg gagtacaaaa taccgtagct gaagatctgt cgaaaaacgg
     1261 cctggaactg gaaagcgttt cacttaccaa ctttaaccag acctcgaaag aacatttcaa
     1321 tcctaacaat gcctttgacg ccgaaggttt aaccaaactg actcaggaga cggagcgccg
     1381 tcgccgcgag cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg agaaaaaccg
     1441 tgatgcactt tcgcgcaagt tggagattga acaacaagaa gcgtttatga cgcttgagca
     1501 ggagcagcag gttaaaaccc gtaccgctga gcagaatgcg aaaattgcgg cttttgaagc
     1561 tgaacgtcgt cgtgaagcag agcagacgcg aattctggct gaacgacaga ttcaggaaac
     1621 agaaatcgac cgcgaacagg ccgtccgctc aagaaaagtt gaagctgaac gtgaagttcg
     1681 cattaaagag atcgaacagc agcaggtcac cgaaatcgct aaccagacga aatcgatcgc
     1741 tattgccgcc aaatcggaac aacagtccca ggcagaagcg cgtgctaacc ttgcactcgc
     1801 agaagcagta agcgcccaac aaaacgtaga aaccactcgc cagacagccg aagccgatcg
     1861 tgccaagcag gtggcgctga ttgccgcagc acaggatgca gaaaccaaag cggttgaact
     1921 gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg cgatcgttga
     1981 gttagccgaa gctacgcgta aaaaaggcct ggcggaagca gaagcacaac gtgcgctgaa
     2041 cgatgctatc aacgtacttt ctgacgaaca aaccagcctt aaattcaaac tggccttgtt
     2101 gcaggcgcta cctgcggtaa tagagaaatc cgtggaacca atgaagtcca ttgacggtat
     2161 caagattatt caggtcgatg gtctgaatcg tggtggtact gcgggggatg caaacacggg
     2221 taatgtgggg ggcggaaacc tggcggaaca agcattatca gccgcactct cttaccgcac
     2281 acaggcacca ctgattgact ccttgctcaa tgaaattggc gtttcaggcg gctcactggc
     2341 tgcattgact tcactcttaa ccccaacaac tcccgtcgcc gaaaacgtag aataaatttt
     2401 cagctatgaa catccctggc tcaaaaagcc ggggattttt tatatctgcg ttccgctaaa
     2461 aggtgcaaat gatcaggctg ttgcagcgtt ttgcgtgatt gctcggggaa ggcaaaactg
     2521 cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag taagcaaagg
     2581 gctgaaacgg gaaagcccct cccgaggaag gggccttaca gaaggaaagg gttatgatga
     2641 agctcgtcat catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa
     2701 tcagaggggg gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc
     2761 agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata
     2821 tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgaccgctc
     2881 ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc atcaacaatc
     2941 ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttatagaag
     3001 gaaagggtta tgatgaagct cgtcatcata ctggttgtgt tgttactgtt aagtttcccg
     3061 acttactaac aactcatcag aggggggaga aatcctccct tacccttgtt cctttactct
     3121 aggttgaaaa gacaacagcg tcaataggcc tgccatgtac gaagcgagat ctgtgaaccg
     3181 ctttccggtt agcctttttt atcctgttgg atcttcttga taatgttagt ggtcgagcaa
     3241 ccgtcttcaa agttgagcac caacacttcg ccgccgttgg cccagacttc tttactcccg
     3301 gcgatctctt ccggtttgta gtcaccgcct ttcaccagca ggtctggcag gatcccggcg
     3361 atcagacgct gcggcgtgtc ttcttcaaac gacactaccc agtcgaccgc ttccagtgcg
     3421 cccagcacaa tcatacgctg ttcgagtggg tttaccgggc gggaatcccc tttcagccgt
     3481 ttggtggagg catcgctgtt gacggcaaca atcaagcggt cacccagctt gcgggcattt
     3541 gccagataag agacgtgccc ggcatgtagg atatcgaaga caccgttggt cattaccact
     3601 ttttcaccac gtttacgcgc tgccactaca gccagcttca gttcctcttc ggtcatcacg
     3661 ccaaagccgg tatctgcgcg tccacgtaca gcgttttcca gctcgatcgg cgaaaccgtg
     3721 gacgtcccca gtttgccgac caccacacca gccgccgcat tggcaaagaa gcaggcttct
     3781 tccagcgaat tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca
     3841 ccggtaacgt catacacttc ctgcgcctga gttggcatat gcagcggcgc tttacccggt
     3901 tgcagcagcg acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg
     3961 gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca
     4021 gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg
     4081 gtaccttttg gatcaatcag caccggaaca cccgctttac gcgccagttg gatcatctgc
     4141 tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc
     4201 agcgcctgat taatccgctc gtgcagcggc tgcggatcaa caccttcgaa accttcttca
     4261 aaatccagac ggatcagctg ttggttgcgg gaaagtaccc gtaatttggt aatggtcgga
     4321 tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt cggccagaga tttactcagc
     4381 gcgcgcgctg catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa
     4441 gcgatattca tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta
     4501 accacgggca ccggcgcttc cggcgagata cgactggagg ggccgtacca gtaacgatcc
     4561 agcatcacat caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc
     4621 attcctgtct cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca
     4681 ccagccactt ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caatcctccg
     4741 gcacatggcc cggcaattcc tgtaatgcca gatgatgaag ttcatcgcga agcgtagtgt
     4801 aagcacgggt cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagtagtt
     4861 ccagaatgcg cacgttgtct gaccagcgcg ttaacttcgg tttttcatga gcgtagcgca
     4921 acaccagata ttgggtaata aattcgatat cggtaattcc cccttcatca gctttgatat
     4981 caaagcgatc gcgatgttta ttgccgagat gagcgcgcat tttctcgcgc atttcccgca
     5041 cttccgtttg cagagtttta ccttcacgcg gcagcgtcat aatctcgcga cgcactgcgt
     5101 caaagtgcgc ggtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat
     5161 gctcccacgt ccaggcctcg tttttctgat aatcggcaaa tgcttctgtg gaggtaacca
     5221 gcattcccgc cgccccggac gggcgcagcc gcgcatccac ttcgtacaaa atgccggaag
     5281 aggttcgcgt gctgaacagg tgcataatgc gttgcgccag acgcagataa aactgtcgtc
     5341 cgtcgatttc ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca
     5401 ggtcaagatc ggaactgtag cctaactccc agccgcccag cttgccgtag ccgaccactg
     5461 caaaaccacg cccttcgcgt tcgttcaggt gattcggctt accgtagcgg gcaaccattt
     5521 gaacccacgc ctgctgaacg acggcatcta tcatggcttc cgccagccag gttaagtgat
     5581 cgctcacttt catcaccggt agcgtaccgg cgatatccgc tgcggcgatg cgtaacagct
     5641 gcgcctgttt gaactgacgc agcgcctcaa gctgttgctc ttcgtcatct tccggcacgc
     5701 gcagcaaata ctggcgcaac tcatcgcggt aggcatcggt cgccgtcggc tggtaaaggg
     5761 tgtttggatc gagcaattca tccagcaata atggataacg cgccagctgg ctggcaatca
     5821 tcggcgacgc tgcacacaga gaaatcaaat gtttaagcgc cgcggggaat tcactgagca
     5881 attctaaata ggtggtgcgg gtaacaatcc ccgccagcaa ggcggtaatg cgcaacagcg
     5941 taacggcagc gtcttcacgc gcacagacat cacttagcag atgcggcatc agatggtcga
     6001 gcacctgacg cccgcgtggc ccgattgtgc gcttatccag ctctttgcgg aaatcggcaa
     6061 tcagcgtcag cacctgtttg cgatcgtcct cgctaagatg cgccagcact ggtgtagtgt
     6121 catcttcctg caacgcatcc tgccacagct cgcgccactg ttccgacagc gactcttcct
     6181 gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca ttggtcatat
     6241 gtgcggtcag cgccccggtc aattgcggcc agtcagcaaa gtccatcgcc cacgccagcc
     6301 gcgcacggtt aagctcatca gaaggaagcg tctgggtttg ttcgtcgtta atgctttgca
     6361 gcaggttttc cagacgccgc aggaacagat acgccactcg caattgttca gcatcgtttt
     6421 cagaaagcag atgcagcgcg gcaatgacgc tgagcgttgg cagtaaagag cgcgactgca
     6481 gcgacggttc gcgcccgccg cgaatgagct ggaacacctg aacgataaat tcaatttcgc
     6541 gaatgccgcc tgcgccgagt ttgatattgt cggtcaaacc acgtcgacgc acttcacggg
     6601 caatcattcc tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga
     6661 aaacaaacgg gcgcagcatc gcacgcaact cgttagcata gacgccttcg ctatcgccca
     6721 taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc tggtaataat
     6781 cttccaacgc ggcaaagctc agcaccagcg ggccactttc gccaaacgga cgcagccgca
     6841 tatccacgcg atagacgaag ccatcctgcg ttggttgatc cagcacttta atcagccgct
     6901 gccccatgcg ggtaaaaaac tgcgcgttat ccagttccca gcgtccaccc tgcgtacaac
     6961 catgttccgg ccaggcaaaa atcagatcga tatcagagga gaaattcagt tccccgccgc
     7021 ccagcttacc catgcctaaa atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg
     7081 ttccccactc gcggcagcag gcgtcatata gccagtcacg cgccgcaaca atcagcgtct
     7141 ccgccagata gctgagctgc tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg
     7201 cccaggcgat acgtaccatg atgcgccgac ggaacagccg cagctcgcgc attaacccgg
     7261 cttcgtcact cacattaatg agcgcctcct gcaaccatgc aacgtaatgc tgccattcgt
     7321 cggcctgcgg cggttggctt tccagttccg tcagccactc tggatgcgca cttatgctgt
     7381 cctgcacaaa atcactgaaa gtaagtactg acttcgcctg tgcactaagt gattcctcgg
     7441 ctaaaggctc tggcagccgc tcaacaatgg tctgccagta ctgctgtaac ggtgaagaga
     7501 gcggcttcat aagcgatttt atccttgcct gattaacgtt ttccgctgtg caaccagaac
     7561 ggttcctgat tgtttgcctc attacggaaa tgttcaattt cgatgcgttg cccggtcgca
     7621 atagcgtgat gcagcccctg ccagttctcc agccaggctt gcgcgacgac aggatcatag
     7681 taacccgcca gcaacagtat tgagtcaata tcacgcgtca ggcgtggcaa ctggtcacgg
     7741 tagcgatcgc ctaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga
     7801 tggatatcgg caaagcgttt gaaggagtcg ctgattttgc cctgcgcttt ggcatctaaa
     7861 aatggctgcc atgctttgct taccaaccat tcggtcaacg ccagcttcgc cattgccgtt
     7921 tcggtagagt agaccgccgt cacggcagaa accgcagaag caatggtcgc ttcgcattgg
     7981 gtcagcaaat cacgtaagtg agtgctcgct ttacgcggca caataccacc gaacagcatc
     8041 agcgtatgac ggaccaagct aatggctgcc agcacctgtt ctttcgccgc atcgttgccg
     8101 cgtacccaca gttcttcatg atactgccat tgcgctaacg ccagctcgag cgccgcttcc
     8161 agcccctgtt ccacatcggc ttttgccgca acatgcaaaa tggtggtcgg tttgatttca
     8221 cgcgccggat tgccctgcgc cagatgatag ccacgcgccg ctttgctcag gctgccctgg
     8281 cgtaatccgg tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg
     8341 ctaagcagct ccagttccag ctcacagata ggttcagcaa attcccccgc tttcacctca
     8401 ccctgatcga gagcgatttc aattcggctg ccatcgaccg ccaccagcca tttttcgcga
     8461 taaaaatcgg tgctgaacag cggttgtacg cgggaagcga gatcggcggg caattcgccg
     8521 ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg
     8581 ttatattccg ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc
     8641 tcatagcgac cgttttcgcc acgaatacgt aagcccatat cgtgcccacg cagccagtta
     8701 tccggcgttt cgtagtaaat attcagtaac tgcacggggt catggtgctc gccgcccagc
     8761 gtattgagat ggtcacgcaa cgcctcaacg gcgctgtgat taacaataaa ctttaattcg
     8821 atttcctgag ccatggcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga
     8881 acttaatgcg atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg
     8941 caatttgacg ggcgtaaaag tttgaagcag tggcaagacc tgacacagag gattccgatt
     9001 gatgtcgaat gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac
     9061 aataacgaca gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc
     9121 gcgactgccg tctcacacgc tgaagaaacg cgctatgttt ctgacgaact gaatacctgg
     9181 gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa
     9241 gtgaccttat tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc
     9301 cgtaccgcct ggatcccgtt gaaacaactt agcactgagc caagcctgcg ctcccgtgtg
     9361 ccagatctgg aaaatcaggt caaaaccctg accgataaac tcaccaatat cgataacacc
     9421 tggaatcagc gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac
     9481 gggttaaaag aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc
     9541 gatgccgcca gcgtgcagct ggatgacaaa cagcgcacca tcatcatgca atggtttatg
     9601 tatggcggcg gcgtgctggg gcttggcttg ctgctcggtc tggtactgcc gcacctgatc
     9661 ccaagccgca aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct
     9721 tattattagg aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt
     9781 ggtgctgttc gggatgcatt gttagggcta ccggtcaaag acagagattg ggtagtggtc
     9841 ggcagtacgc cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct
     9901 gtgtttctgc atccgcaaac gcatgaagag tatgcgctgg cacgtaccga acggaaatcc
     9961 ggctccggtt acaccggttt tacctgctat gccgcaccgg atgtcacgct ggaagatgat
    10021 cttaagcgtc gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc
    10081 gacccgtaca acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct
    10141 tttggcgaag atccgttacg cgtactgcgc gtggcgcgtt ttgctgcgcg ttatgcccac
    10201 ctcggttttc gtattgccga tgaaactctg acgctgatgc gcgagatgac ccatgcgggt
    10261 gaactggaac acctgacgcc tgaacgggta tggaaagaga cagaaaacgc cctcaccacc
    10321 cgcaatccac aggtgttctt ccaggtactg cgcgattgcg gcgcactgcg cgttttattc
    10381 ccggaaattg acgccctgtt tggtgttccg gcccctgcca ggtggcatcc ggaaatcgac
    10441 acgggtattc ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggtcgat
    10501 gtccgtttca caactttatg ccacgatctc ggtaaaggac tgacgccgcc agaactctgg
    10561 ccgcgtcatc atggtcatgg cccggcgggt gttaagttag tggaacaact atgccagcgt
    10621 ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg tggctgagtt tcacgatctc
    10681 atccacacct tcccaatgct gaacccaaaa accatcgtca aattatttga ttccatcgat
    10741 gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgacgtgcgc
    10801 ggcagaaccg gttttgaatc agcggactac ccgcaaggcc gctggttgcg cgaagcctgg
    10861 gaagtggcgc agtcagtgcc gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag
    10921 attcgcgagg agctgacccg acggcggatt gcggcggtag ccagctggaa ggaacaacgt
    10981 tgcccaaagc ctgagtgagt attgggcatt ttgccggatg cggcgtaaac gccttatccg
    11041 gctacatgtc aacgacagtt gtaggcctga taagacgcgc aagcgtcgca tcaagcgttg
    11101 attgccggac gcggcgtgaa cgccttattc ggcctacgca atcgtggaca acgccaggca
    11161 tccgacacta ttcctcaatt aaaagaacac gacatacacc gcagccgcca caataaagcg
    11221 ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc
    11281 tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga tatcgccgct
    11341 tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca tcatcggcac
    11401 cgccagcagg aacgaaaact cggaagcagc gtaacggctc acccccatca gcatcccacc
    11461 tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact ggaaacaacc
    11521 aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg
    11581 cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat acatcacatt
    11641 tatcgggtta aacaatgact taatcgtgtc gtggaacaac agccccagta ccaccgccgg
    11701 aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttctc cttcgtgctg
    11761 caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga acatcactac
    11821 taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg tgtcgccctc
    11881 aaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg gcagaaattc
    11941 tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca tatcgctcat
    12001 caataaaccc ctaaattata aaaatgtaac gcttgtccga actactgcag tatgaccagg
    12061 ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat tattgccacg
    12121 ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc tgagtttgat
    12181 acgcacccac ggcgagttga agcgtgctaa cagcagctct gccacctctt ccgccacgcg
    12241 ctccaccagc gcaaaacgtg ccccctcgac gtggctgacc accgtttctg caatgtcagc
    12301 gtagctgagg caatccgcca cattatcgct ttttgccgct ttacggttat cccacgccat
    12361 ttcgatatcg aacactaatt tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt
    12421 ggtgattacc gaaagttgct ctataaatac aatatccatc acgacctgct cgctttttgg
    12481 ctaacccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgtttaaca
    12541 cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg
    12601 tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcctgtgtgg gctgcccgat
    12661 ccgcgaacca gcggctccgg taatcctggc gcaactaacg tgttacgtat aggtggcaaa
    12721 ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc
    12781 gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt
    12841 ggacacatct ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt
    12901 ggtgccatcg cccccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg
    12961 accgtgctat tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg
    13021 ttttatgtct ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg
    13081 atcctgctgc gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc
    13141 tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat
    13201 gacctgatag ccttcatcgg gtttgcccag ccgttgctgg caccatgccg ccagaaattc
    13261 cacgcagaca cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt
    13321 ggcgctttgt gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa
    13381 cacatcccac tcggaacgca gcataatccc tttgccctcc agcgcccatt gcagcacaat
    13441 ctcgccgcta ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt
    13501 ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg
    13561 acttaattcc tgtagggatt gtggctgcgg atatttttgc agatactcag gtgctgcgca
    13621 taatattctt ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt
    13681 aatacgaata tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc
    13741 aaaatacacc tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg
    13801 gctgcgccca aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat
    13861 ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta
    13921 acaacgttgg ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt
    13981 tgccagcgta ttttcaagga tttggatccg cttggtgaca aatgcaggcg tttgcccaag
    14041 cgtcgccgct gcagcactaa aactcccgct gtggacgatt tctaccagta cctgcaagtc
    14101 tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg
    14161 tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt
    14221 gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag
    14281 tggtagtttg tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga
    14341 gcgaaagtaa taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg
    14401 ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg
    14461 ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag
    14521 cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag
    14581 tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag
    14641 aggcgaccat caaagcgccg ctgcgtcaca atgcggtaga aatttttgac gaagtaaaca
    14701 ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggatatcgtc cccgacggtg
    14761 acgatgcgga aatcgaagtt tacatggcag gcggcggctg cacgctacca ggccgctcga
    14821 aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct
    14881 ccaccctcgc agtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg
    14941 tggaaaccgc cgccgtactc acgcgtaaag ccattttgcg cccgattggc agccgccacc
    15001 ccaatccaaa agcggcagag ctggagctac gcctggaaga aggactcaac cgtctgggga
    15061 ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtgcatatc gaatctgccg
    15121 cccgccatcc gtcaaccatc ggcgttgctg tttctaccgg ttgctgggcg catcgtcgcg
    15181 gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    15241 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcacgtcgg
    15301 cgatgtgatc tatctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgatt
    15361 gatcgaactg aaacgtccgc tcccttacga tctcaacggc aaagcgattt tccacgctgg
    15421 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggtc cgacaaccag
    15481 tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt
    15541 cggcaaaggc ggaatggggc cgctgaccga agaaggctgt cagaaattca aggcgctaca
    15601 tgtaattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga
    15661 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    15721 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    15781 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    15841 caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa
    15901 accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct
    15961 aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg
    16021 cgtaatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat
    16081 ctccatcatc gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc
    16141 aggctttaaa ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt
    16201 tatctggctg attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggctggg
    16261 gcgacgtatc gcgctgattc tggtgaaaaa gatggggcat cgcacgctgt ttcttggcta
    16321 tgcggtgatg ttctccgagc tgatcctggc acctgtcaca ccgtccaact cggcgcgtgg
    16381 tgcggggatt atctatccca tcatccgtaa cctgccgccg ctctatcaat cacaaccaaa
    16441 cgacagcagt tcgcgcagta ttggctcgta catcatgtgg atggggattg ttgccgactg
    16501 cgtgaccagc gccattttcc tgacggcgat ggctcctaac ttgctgttaa ttggactgat
    16561 gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct
    16621 cagtatttta ctggtcctgc tggttccctg gctggcttac gtgctgtacc cgcctgtact
    16681 gaagtcaggc gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct
    16741 ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc tgtggatttt
    16801 cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcgc tgatgctgct
    16861 tctgcgcatt atcagttggg acgatattgt cagtaataaa gcggcgtgga acgttttctt
    16921 ctggctggcc tcgcttatca ccctcgcgac cggactcaac aacaccggtt ttattagctg
    16981 gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc
    17041 gttgattgtg gtgttttatc tactgcgcta cttttttgcc agcgccacgg cgtatacctc
    17101 cgctctcgca ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt
    17161 gttctgcctg atggttggtg cggcaattgg tctggggagc attcttacac catacgccac
    17221 cggccccagt ccgatttact acggcagtgg ttatctgcca acggcggatt actggcgact
    17281 gggggcgatt tttgggctga tattcctcgt attgctggtg attaccggct tactgtggat
    17341 gcccgtggtg ttgctttaag taaggacggt gccggaagta actctccggc acccgatagt
    17401 tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg
    17461 ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag
    17521 tacaaaattc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca
    17581 gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc
    17641 ccgtctgatc cagcgcacgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa
    17701 aggcgcgggc gatgtcagca cgcgtctggt cgtcggtgcc gttgtcacga atggtatttg
    17761 ccgcgaaggt tttcaggccg gagaagctga aatccagccc cggacggtcg gtcatcggac
    17821 gcgggaaaac aaagcgcccg gcagtaccct gcgccgccat tttcgacagt aacggtccgc
    17881 caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat
    17941 cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat
    18001 gaccgccgga aaccagcagc gcaacaaacg gaaattccgg cgggttatct tccagcatcg
    18061 gcgctaacag atgcccttcc atatggtgta cagggatcgc cggaacgttc caggcaaacg
    18121 ccagagaacg ccccacggtc gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg
    18181 tataggccac agcatcaatg tcttttgccg ttaaaccaga ctcctttagc gccgcctgga
    18241 tcaacggtac ggttttacgc acatgatcgc gggaggccag ttcaggcaca acgcccccgt
    18301 agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt
    18361 cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt
    18421 gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg
    18481 ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa
    18541 aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc attttgaaat
    18601 aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc acatgccggt
    18661 aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca agcgttcctg
    18721 cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa aaccgactac
    18781 cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac tggctcgcga
    18841 aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc gatcagaccg
    18901 agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc gtttatgaat
    18961 tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa tgatctgctg
    19021 gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa gcagggcaag
    19081 aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtccttcac cgttaacggt
    19141 gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat cgacttcctg
    19201 atgaactacg acaagcttga gttcgtcgaa acggtcgaag agctggcggc aatgcacaat
    19261 cttgaagtgc catttgaagc aggtagcggc cccagccaga tcgagcgcca tcagcggcaa
    19321 acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt acaacaacct
    19381 gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga ggttatcgct
    19441 cgctttgcga ttggttttgc gcctcccggc tgggacaacg tcctgaagcg gtttggcggc
    19501 aatccagaaa atcgccagtc attgattgat gcggggatgt tggtcactaa cgatcaggga
    19561 cgcagttacg atcgtttccg cgagcgggtg atgttcccca ttcgcgataa acgcggtcgg
    19621 gtgattggtt ttggcgggcg tgtgctgggc aacgataccc ccaaatacct gaactcgccg
    19681 gaaacagaca ttttccataa aggccgccag ctttacggtc tttatgaagc gcagcaggat
    19741 aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt ggcgctggcg
    19801 caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc cgatcacata
    19861 caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga ccgtgcaggc
    19921 cgtgatgccg catggcgagc gctggaaacg gcgctgcctt acatgacaga cggccgtcag
    19981 ctacgcttta tgtttttgcc tgatggcgaa gaccctgaca cgctggtacg aaaagaaggt
    20041 aaagaagcgt ttgaagcgcg gatggagcag gcgatgccgc tttccgcatt tctgtttaac
    20101 agcctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt gagtacgctg
    20161 gcactgccat tgatatcgca agtgccgggc gaaacgctgc gaatatatct tcgtcaggaa
    20221 ttaggcaaca aattaggcat acttgatgac agccagcttg aacgattaat gccaaaagcg
    20281 gcagagagcg gcgtttctcg ccctgttccg cagctaaaac gcacgaccat gcgtatactt
    20341 atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct tgagaatctg
    20401 gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac ttgtctctcc
    20461 cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa taatgctgcc
    20521 acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc tgagcaaacc
    20581 ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca ggaagagtta
    20641 atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct ctggacatta
    20701 aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg atcgggaagc
    20761 ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg taattatcgt
    20821 tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg gtcaatcgcc
    20881 caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac ccgcagtcac
    20941 agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat gccgaggtca
    21001 atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc atccaaatga
    21061 tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat ctgatgctgg
    21121 ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt tccagcgtgg
    21181 aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa atgggcaccg
    21241 ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa gacgggatca
    21301 accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg ctggaacagt
    21361 acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc tttgttgacc
    21421 cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag ctttcccagg
    21481 aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac agcgccgatg
    21541 atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaactg cgcgctcagt
    21601 acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc gctcaggaag
    21661 agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag cagtttgact
    21721 acctggtcaa cagtatgcgc gtcatgatgg accgcgttcg tacgcaagaa cgtctgatca
    21781 tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattact ctgtttaccg
    21841 gcaacgaaac cagcgatacc tggttcaacg cggcaattgc gatgaacaag ccgtggtcgg
    21901 aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcagaaactg cagcagattg
    21961 aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt atgtccatcg
    22021 gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta cgtctggtta
    22081 tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg attcaggaag
    22141 gcaacatcgg tctgatgaaa gcggttgata aattcgaata ccgccgtggt tacaagttct
    22201 ccacctacgc aacctggtgg atccgtcagg cgatcacccg ctctatcgcg gatcaggcgc
    22261 gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac cgtatttctc
    22321 gccagatgct gcaagagatg ggccgtgaac cgacgccgga agaactggct gaacgtatgc
    22381 tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca atctccatgg
    22441 aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag gataccaccc
    22501 tcgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaaca cacgacgtgc
    22561 tggctggcct gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt atcgatatga
    22621 acaccgatca cacgctggaa gaagtgggta aacagttcga cgttacccgt gaacgtatcc
    22681 gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct gaagtgctgc
    22741 gtagcttcct ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca cccggcacct
    22801 gtccctttgc acaaacgcca ccttttcggt ggcgtttttt atcgcccacg caccaccagc
    22861 gcctggtcca gctcgcgata cgcttcaacc agtttctcca gtgaaacgcg acttaaaccg
    22921 ctgggatttg gcagcaccca aatctgcgtc gaaccaatgc tgagcgtttg tttcccccac
    22981 tgtgcaccac gctggctgaa tccctgttca tatgcttgtt tgcccagaat cgccaacgct
    23041 tgcggctgat aatcttcaat tttttcaatc agcttacgcc cacctgcgtg tagctcttgc
    23101 tttgaaattt cattggcttg caccgttggc ctgtctacca gtttggtgac gccacaacga
    23161 taatccagca gatgctgtgc ctcctgcggc ttcaactgat ggtcggtaaa cccggcctga
    23221 tatatcacct tccagaagcg atttgccgga tgagcaaagg gaaaaccagt cccggcggat
    23281 gaaagcccag ggttgatacc gcaaaacacg acccgtaacc ctggagccaa aatatcctca
    23341 accatattta ctcctttcta acatcaccag gaaaagtata acggattgaa aatacattgt
    23401 ttataaaaac agcaggcgcg cggtaatggc tggattgcga cacggagtta ctttataatc
    23461 cgctaccatg gccccttagc tcagtggtta gagcaggcga ctcataatcg cttggtcgct
    23521 ggttcaagtc cagcaggggc caccagatat agcaaaggct gacgagaaat cgtcagcctt
    23581 tttcttttta tatatcagtt actttgcgtg ccagtaggcc gctgcacgta cccgctgtgg
    23641 gtcatactgt tccgcttcaa agcggcggct taaattctta acgactttac cttcgccggt
    23701 tatccagatg aagtaatcat cggcagggat ttgcatctgc gccagacgcg catctaccgc
    23761 ctgctcatca tgtgccagcc attcgatatt aaaaccatca aggtgcgcga gataatcctg
    23821 acaggcgtta tcccgcacgc taaccagcgc actaacttgc ggtttaacgg caagtttgct
    23881 caacgtttcc aggcggcggc gcaatgcggg cattccggat tcatcgcaga catacagctg
    23941 atacgcgtaa tcttccggca ccaccagcga accgcgcgga cctgccaccg taagtttatc
    24001 gcccggttgc gcctgcatcg cccagccgct ggcgacccca ccgtcgtgaa taaagaaatc
    24061 gatcgccagt tcatggcgta gttcgtcata cagcggcgta tagtcacgcg acggtgggcg
    24121 tggtccttcc ggccagacga tgccctcttc cgttaccgtt ggcggcacaa agtgagcgtc
    24181 aggttgagga aagaagagtt tgctgtgatc gtcaaagccg cgcgatgtaa aaccgtccag
    24241 cgcctcgccg ccaaggacaa tgcgctgaaa accggcgctg atacgctcaa cgcgtagcac
    24301 agtcaattca cggaagcgca gatcattgcg aacgcgctgc gggtagcggg gggagttatt
    24361 cattgttatc gccttcgtga tggtaatcag atatatctaa ataattttcg caaatgataa
    24421 tgattgttaa tcatgataaa tgcaagcgat ttgtagaact gatatgtcta tagtctgaca
    24481 agacgaaccg cctcttctca ggcatcatta ctcaacgccg gatgcggcgt gaacgcctta
    24541 tccggcctac gtgtgagatg agtccacatt taaaaatcat ttttattctt gcaagagtgc
    24601 tcacatcaga tataaattag atatatctaa ttaagcaaaa ggaggctgat atgagccatc
    24661 atcacgaagg gtgttgtaaa catgaaggcc agccacgcca tgagggctgc tgcaaaggtg
    24721 agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt caatgttgcg
    24781 gtggtcgcca cggtcgcggc ggcggtcgtc ggcaacgttt ctttggtcac ggtgaattgc
    24841 gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgaa ttgattaaag
    24901 cgattgagaa tctaacccaa gggaattaca ccccaagccc gggcgtcatc tacccgacgc
    24961 tggattttct gcaggagcag tcgctgatta ccatccgcga agaggaaggt ggtaagaagc
    25021 agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa caggtggaga
    25081 tgattgaaga acgcatcaaa gcgcgttgcg ttggcgctgc gctgcgccag aacccgcaaa
    25141 tgaagcgggc gctagataat tttaaagcgg tgctgaattt acgcgtcaac cagagtgata
    25201 tcactgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct tttgatatta
    25261 cgcaactgga ttaatcgccg catccgccag tggcgcggtg caattgccgg atgcgacgct
    25321 tgacgcacct tatccggcct acacccgcta cacaccccgt aggcctgata agatgcgcca
    25381 gcatcgcatc aggcatcgtg tatcaaatac cggatccggc ataccgatta atgcagcacc
    25441 gtcaccgcgt cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc
    25501 tcttccacca gctcggcatt tttctgggtg atgaggttaa gctcatccac tgcacgggtc
    25561 aggctggaaa gaccatcggc ctgttccagc gttgaatggc taatctgggc aatcaactgg
    25621 gtgacgtttt tcacctgtgc cacaatatct tccatcgtac gtccggcggc gtgtacctgc
    25681 tgcgaaccgg attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca
    25741 gcattggcgc tgcggctggc taaatgacgc acttcccccg ccaccaccgc aaaacctttg
    25801 ccctgttcgc cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga
    25861 aacgcaatat cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata
    25921 tcgtccattg tcttgatcac cgtggtcatc gcctccccgc cctgcactgc cgcattactg
    25981 gcggtgattg acagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc
    26041 gccatttggt tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt
    26101 tcgttcagtt catcggtgcc tttcgccagc gtctcactgc cgtttctgac actggacacc
    26161 tggcttgaga catcgttaat taaccaacgg cacatcaggc caagctgccc taccgcgcgt
    26221 aatgtcagcc ccagctcatc gctgcgattc agatgctcaa cactattacg ctctccggtc
    26281 gccaccttca gtgcctgacg ggcgacattt tctatcgggc ggacaatctg ccattcaaaa
    26341 caggcgcttg ccaacaatac cactaacgca cagaggatat acgtcaccac cggggcagca
    26401 acaaaccaaa gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc
    26461 cagcgaagcg gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta
    26521 tgaatacgct taccggtacg tccggcgttc agcgctttgt acagcggctc caccgccgcg
    26581 atctcttcat ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc
    26641 accatcggta ccgcattggc ccgcacccaa taatggtcac catttttgcg gcgatttttc
    26701 acgatgccgc tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct
    26761 tttggcatat ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta
    26821 aagccgctca cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta
    26881 gtggacatca gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga
    26941 gaagacatgt tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg
    27001 tcggccccgc tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta
    27061 aagcacaaat ctaattcgtt gatttaaaat actttcactc tgttactata cgaaaacgtt
    27121 aattatcttg cccaaaaatc aggcagttaa tgccctgaaa gcgtgcattt gcgcagcaat
    27181 tatcaaatcc atacccgaca aaaaccgtgc aaaataacaa cagatgttaa caaatagcat
    27241 taaatattgc gcaaatgata accacatttg tgtttatccc gattttcgcg atcgcagccg
    27301 gagtggcgca atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc
    27361 atattttgaa caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga
    27421 atctcattga aaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga
    27481 ttgaatactt caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg
    27541 ccggcgggga ttacggagcc gtagagtggc aagcgggagg tttaaatacg cttgtcgaca
    27601 cccagggaca ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc
    27661 gtaatccagt tgtggtttcc gccgtacaga atcaacttgc gaaacaacca ctgcacagcc
    27721 aggagctact cgatccgtta cgggctatgc tggcgaaaac ccttgctgcg ctaacgccag
    27781 gtaaactgaa atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcggcgctga
    27841 agctggcgaa ggcttatcag tcgccgcgcg gcaaatttac ttttattgcc accagcggcg
    27901 cgttccacgg taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac
    27961 cgtttatgcc actgctgcca gggttccgtc atgtgccgtt tggcaatatc gaagccatgc
    28021 gcacggctct taatgagtgc aaaaaaaccg gtgatgatgt ggccgcggtg atcctcgaac
    28081 cgattcaggg cgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtgcgta
    28141 agctgtgcga tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc
    28201 gcacgggcaa gatgttcgcc tgcgaacatg agaacgtaca gccggacatc ctgtgcctgg
    28261 caaaagccct cggcggcggt gtgatgccga ttggcgcaac catcgccact gaagaggtgt
    28321 tctcagttct gttcgacaac ccgttcctgc ataccaccac ctttggcggc aacccgctgg
    28381 cctgtgcggc ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg
    28441 ctgagcaaaa aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg
    28501 atctggtaca ggaagcgcgt ggtaaaggga tgttaatggc gattgagttt gttgataacg
    28561 aaatcggcta taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc
    28621 tcaataacgc caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg
    28681 aactcgtgat caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag
    28741 cgtagtatca aatcggatgg caatacgacg tcgccatccg atctttttat acgatacgta
    28801 cgcccgcagg catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt
    28861 ccgcacacaa cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc
    28921 ataagaccac caccgttttt cccatcaact cttcttcgct gtagtacggc accaggctgg
    28981 tcacggtttg cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt
    29041 tttcatggcg tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag
    29101 cgtaagccac ggtttccatt gcttcctccc ttcgagtaaa attttactaa actatagcaa
    29161 aagtttttct caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtgag
    29221 ccgctgtgtt accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc
    29281 cgataagcac ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg
    29341 gcaacactaa aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc
    29401 ttaaatgacg atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc
    29461 gccgaaaagc tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac
    29521 caacaccata ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac
    29581 tatctggcga tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc
    29641 aactgttatg aacacagcgg attaccagac attaaaaacg tcaccggtat tttaattgtc
    29701 ggcaaaccca cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt
    29761 atcgactttc acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc
    29821 agtaaagaaa tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc
    29881 ggtgaagatg agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga
    29941 ctgaaacaag tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt
    30001 tatgaactgg caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct
    30061 tccgattcca ttgctatcgg cgtactgcgg gcgattcatg aacgtggtct gaacatccca
    30121 caggatattt cgcttatcag cgttaacgat atccccaccg cgcgatttac ctttccgccg
    30181 ctctccaccg tgcgcatcca ttccgaaatg atgggaagtc agggcgtaaa cctggtgtat
    30241 gaaaaagccc gcgatggtcg cgcgctgccg ctgttagtct tcgttcccag caaattaaaa
    30301 ctgcgcggca cgacccgtta aatcccctta cacactgtcc ggcaatcgtt tttgccgggc
    30361 agtgctgccg tttattttcg tgatccagtt aaagtaaatg catttacccg ctacttttta
    30421 gtaaaaattt tactaaactc cccagcaatt acacaaacta ccatcaccat gaatggttcc
    30481 gatttctctc taccgggagg ccctatgaat cgctgggaaa acattcagct cacccacgaa
    30541 aaccgacttg cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca agcgcgtacc
    30601 tttgcccgcg aaacgagcag cctgtttctg cccttaagcg gtcagtggaa tttccacttt
    30661 tttgaccatc cgctgcaagt gccagaagcc ttcacctctg agttaatggc tgactggggg
    30721 catattaccg tccccgccat gtggcaaatg gaaggtcacg gcaaactgca atataccgac
    30781 gaaggttttc cgttccccat cgatgtgccg tttgttccca gcgataaccc aaccggtgcc
    30841 tatcaacgta ttttcaccct cagtgacggc tggcagggta aacagacgct gattaaattt
    30901 gacggcgtcg aaacctattt tgaagtctac gttaacggtc agtatgtggg tttcagcaag
    30961 ggcagtcgcc tgaccgcaga gtttgacatc agcgcgatgg ttaaaaccgg cgacaacctg
    31021 ttgtgtgtgc gcgtgatgca gtgggcggac tctacctacg tggaagacca ggatatgtgg
    31081 tggtcagcgg ggatcttccg cgatgtttat ctggtcggaa aacacctaac gcatattaac
    31141 gatttcactg tgcgtaccga ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa
    31201 gtggtgctgg aaaatctcgc cgcctcccct gtcgtcacga cgctggaata taccctgttt
    31261 gatggcgaac gcgtggtgca cagcagcgcc attgatcatt tggcaattga aaaactgacc
    31321 agcgccagct ttgcttttac tgtcgaacag ccgcagcaat ggtcagcaga atccccttat
    31381 ctttaccatc tggtcatgac gctgaaagac gccaacggca acgttctgga agtggtgcca
    31441 caacgcgttg gcttccgtga tatcaaagtg cgcgacggtc tgttctggat caataaccgt
    31501 tatgtgatgc tgcacggcgt caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt
    31561 ggaatggatc gcgtcgagaa agatctccag ttgatgaagc agcacaacat caactccgtg
    31621 cgtaccgctc actacccgaa cgatccgcgt ttttacgaac tgtgtgatat ctacggcctg
    31681 tttgtgatgg cggaaaccga cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc
    31741 cgtattaccg acgatccgca gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatt
    31801 cacgcgcaga aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga atccggctat
    31861 ggctgtaaca tccgcgcgat gtaccacgca gcgaaggcgc tggatgacac gcgactggtg
    31921 cattacgaag aagatcgcga tgctgaagtg gtcgatatta tttccaccat gtacacccgc
    31981 gtgccgctga tgaatgagtt tggtgaatac ccgcatccga agccgcgcat catctgtgaa
    32041 tatgctcatg cgatggggaa cggaccgggc gggctgacgg agtaccagaa cgtcttctat
    32101 aagcacgatt gtattcaggg acattatgtt tgggagtggt gcgaccacgg gatccaggcg
    32161 caggatgaca acggcaatgt ctggtataaa ttcggcggcg actacggcga ctatcccaac
    32221 aactataact tctgtcttga tggtttgatc tattccgatc agacgccggg accaggcctg
    32281 aaagagtaca aacaggttat cgcgccggta aaaatccacg cgctggatct gactcgcggc
    32341 gagctgaaag tcgaaaataa actgtggttt accacgctgg atgactacac cctgcacgca
    32401 gaggtgcgcg ccgaaggtga aacgctcgcg acgcaacaga ttaaactgcg cgacgttgcg
    32461 ccgaacagcg aagccccctt gcagatcacg ctgccgcagc tggacgcccg cgaagcgttc
    32521 ctcaacatta cggtgaccaa agattcccgc acccgctaca gcgaagccgg gcattctatc
    32581 gccacttatc agttcccgct gaaggaaaac accgcgcagc ctgtaccttt cgcaccgaat
    32641 aacgcccgtc cgctgacgct ggaagacgat cgtttgagct gtactgttcg cggctacaac
    32701 tttgcgatca ccttctcaaa aacgagtggt aaaccgacat cctggcaggt aaatggcgaa
    32761 tcgctgctga cccgcgagcc aaagatcaac ttcttcaagc caatgatcga caaccacaag
    32821 caggagtacg aagggttgtg gcagccgaat catttgcaga tcatgcagga acatctgcgc
    32881 gactttgccg tagaacagag cgatggtgaa gtgttgatca tcagccgcac ggttatagca
    32941 ccgccggtgt ttgacttcgg gatgcgctgt acctacatct ggcgcatcgc tgcagatggc
    33001 caggttaacg tggcgctttc cggcgagcgt tacggcgact atccgcacat cattccgtgc
    33061 atcggtttca ccatggggat taacggcgaa tacgatcagg tggcgtatta cggtcgtgga
    33121 ccgggcgaaa actacgccga cagccagcag gctaacatca tcgatatctg gcgcagcacc
    33181 gtcgatgcca tattcgagaa ctatcccttc ccgcagaaca acggcaaccg tcagcatgtc
    33241 cgctggacgg cactgactaa ccgccacggc aacggtctgc tggtggttcc gcagcgccca
    33301 attaacttca gcgcctggca ctatacccag gaaaacatcc acgctgccca gcactgtaac
    33361 gagctgcagc gcagtgatga catcaccctg aatctcgacc accagctgct tggcctcggc
    33421 tccaattcct ggggcagcga ggtgctggac tcctggcgcg tctggttccg tgacttcagc
    33481 tacggcttta cgttgctgcc ggtttctggc ggagaagcta ccgcgcaaag cctggcgtcg
    33541 tatgagttcg gcgcagggtt cttttccaca aatttgcaca gcgagaataa gcaatgagga
    33601 tcatcgataa cttagaacag ttccgccaga tttacgcctc tggtaagaag tggcaacgct
    33661 gcgttgaagc gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg
    33721 actcattgac ttaccgcgtg gagacagact ccgcgaccga tgcgctattt accgggcatc
    33781 gacgctattt tgaagtgcat tactacctgc aagggcagca aaaaattgaa tatgcgccga
    33841 aagagacatt acaggtcgtg gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag
    33901 gctgcggaga aaccgttgag gtcgacgaag ggcaaatcgt tatttgcgat atccatgaag
    33961 catatcggtt tatctgcaat aacgcggtca aaaaagtagt tctcaaagtc accatcgaag
    34021 atggttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc gccaccgcta
    34081 ccctactcat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa tcggcaaatt
    34141 cggcttgctc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta tcaataataa
    34201 tattgagctt ggactggcct cggcaccgat gtttttcctc gcgacgattt tttattttat
    34261 tcccttctgt ctgatcatcg cagaatttgt ttcgttaaat aaaaactcag aagccggtgt
    34321 ctacgcgtgg gtaaaaagtt cgctgggcgg acgttgggca tttattactg cctataccta
    34381 ctggttcgta aacctgttct ttttcacctc gctgttgccg cgcgttattg cttatgcttc
    34441 gtatgccttc ctcggctatg aatatattat gacgccggtt gccaccacca ttatcagtat
    34501 ggtgctgttc gccttctcca cctgggtttc caccaacggg gcgaaaatgt tggggccaat
    34561 tacctccgtc acttcaacgc tgatgctgct gttaacgctc tcctacattt tactggcagg
    34621 tacggcgctg gttggcggcg tacagcctgc tgaccccatc accgttgacg cgatgatccc
    34681 gaacttcaac tgggcgttcc tcggcgttac cacctggatc tttatggccg caggtggcgc
    34741 ggagtccgtc gctgtgtacg ttaacgacgt caaaggcggt tcgaaatcgt tcgttaaagt
    34801 gatcatcctc gccgggattt ttatcggtgt actatattcc gtctcctcgg tgctgattaa
    34861 cgtcttcgtc agcagcaaag agttgaaatt tactggcgga tcggtgcagg tattccacgg
    34921 catggcggcg tattttggtc tgccggaagc gttgatgaat cgctttgtcg gtctggtgtc
    34981 ctttaccgca atgttcggtt ccctgctgat gtggaccgca acgccggtga aaattttctt
    35041 ctccgaaatc ccggaaggca tctttggtaa gaaaaccgtc gaacttaacg aaaacggcgt
    35101 tccggcgcgc gcagcgtgga tccagttcct gatcgtcatc ccgctgatga ttatcccgat
    35161 gctcggttcc aatactgtgc aggatctgat gaatactatt attaatatga ccgccgcagc
    35221 gtccatgctt ccgccgttat tcatcatgct ggcttacctg aatttacgcg ccaaattaga
    35281 tcacctgcca cgcgatttcc gtatgggctc ccgccgcacc ggtattatcg ttgtttcaat
    35341 gctgattgcg atatttgccg tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat
    35401 tctgaccatc attttttata acgtcggcgg tattgttata ttccttggct ttgcgtggtg
    35461 gaaatacagt aaatatataa agggattaac ggctgaagag cgccatattg aagcgacgcc
    35521 agccagcaat gttgattaaa cagactaata aaaaatgagc aggcattttc cctcttcctg
    35581 gatgaggaga gggaaaatag tttctgcctt tatattttta taattacaac gataaaaggc
    35641 tgtacttttt ctttagctca tggattaaca caatgaaatt aatcactgca ccatgcagag
    35701 cattacttgc tctgccgttt tgctacgcct tttctgcggc aggagaagaa gcacgtccgg
    35761 cagaacatga cgacacaaaa acccccgcaa ttacctcgac atcttctcct tcatttcgtt
    35821 tttacggcga attaggggtt ggtggatata tggatttaga gggtgagaat aaacataaat
    35881 acagcgacgg cacctatatt gaaggtggcc tggagatgaa gtacggctcc tggttcggcc
    35941 tgatttacgg cgaaggctgg accgtgcagg ccgaccacga cggcaatgcc tgggtgcctg
    36001 accatagctg gggcggtttc gaaggtggga ttaaccgttt ctatggcggt tatcgaacca
    36061 atgatggcac cgaaatcatg ctcagtctgc gtcaggattc ctcgctggat gacctgcaat
    36121 ggtggggcga cttcacaccc gatctgggct acgtcattcc caatacccgc gacattatga
    36181 ccgcgctgaa ggtgcagaac ttaagcggca actttcgtta tagcgtcacc gccacacctg
    36241 ccggacatca tgacgaaagc aaagcatggc tacattttgg caagtacgat cgctacgacg
    36301 acaaatacac ctatccggca atgatgaacg gttacatcca gtatgacctt gccgaaggca
    36361 tcacctggat gaatggtctg gaaattaccg acggcacagg acaactctat ctcacgggac
    36421 tgcttacccc taactttgcc gctcgcgcct ggcaccatac cggacgtgcc gacgggctgg
    36481 acgtaccggg aagtgaaagc gggatgatgg tgagcgccat gtatgaagcg ttaaagggcg
    36541 tttatctctc caccgcttac acctacgcca aacatcgccc tgaccacgct gacgatgaaa
    36601 ccacctcttt catgcagttt ggtatctggt acgaatatgg cggcggacgt ttcgcaacgg
    36661 cttttgatag ccgcttctac atgaaaaatg cctctaacga tcccagcgac caaatcttcc
    36721 tgatgcaata tttctactgg taataaggac tgtaatttca tgaaaatcaa aactatttta
    36781 acgccagtaa cctgcgctct gctgataagt ttttccgccc atgccactaa cgccgacaat
    36841 tataaaaacg tgattaaccg tactggtgcg ccgcagtaca tgaaggatta cgattacgac
    36901 gatcaccagc gttttaatcc gtttttcgat ctcggagcct ggcatggtca tctgttgcca
    36961 gacggcccga acaccatggg cggctttccg ggcgttgcgc tgctgacgga agaatacatc
    37021 aactttatgg ccagcaattt cgaccgcctg accgtctggc aggacggcaa gaaagtcaac
    37081 ttcacgctgg aggcatacaa tattcccggc gcgctggtgc aaaaactgac agcaaaagat
    37141 gtgcaggtcg aaatgactct gcgcttcgcc acgccgcgca cgtcactact ggaaactaaa
    37201 atcatcagca ataaaccgct ggatctggtg tgggatggcg aactgctgga aaaactggaa
    37261 gcgaaagaag ggaaaccgct ttccgataaa accattgctg gcgaataccc tgactaccag
    37321 cgcaaaatca gcgccacccg tgatggcctg aaagtcacct ttggcaaagt gcgcgccacc
    37381 tgggatctgc tgacctccgg tgaatcagaa tatcaggtgc ataaatccct gccggtgcag
    37441 actgaaatca acggcaatcg ctttaccagt aaggcgcata tcaacggttc gacgacgctc
    37501 tataccacct attcccatct gctgacctct caggaagtta gcaaagagca aatgcagatc
    37561 cgcgatattc tggcgcgtcc ggcgttttat ctcaccgcct cgcagcaacg ctgggaagag
    37621 tatctgaaga aagggttaac caatccggat gcgacgccgg aacagacacg cgtcgcggtg
    37681 aaagcgatcg aaacgctcaa cggtaactgg cgctcgcctg ggggtgcggt gaaatataac
    37741 accgtcacgc cgtcggtgac cgggcgctgg ttctccggca atcagacctg gccgtgggat
    37801 acctggaagc aggcgtttgc gatggcgcat ttcaatccgg acatcgccaa agagaatatc
    37861 cgcgcggtct tctcctggca gatccagcct ggcgacagcg tgcgtccgca ggatgtgggc
    37921 tttgtccccg acctgatagc gtggaatctt agccccgagc gtggtggcga tggtggcaac
    37981 tggaacgaac gaaataccaa acccagcctt gccgcctggt cggtgatgga agtgtacaac
    38041 gtcacccagg ataaaacctg ggtggcagag atgtacccga aactggtggc ctatcacgac
    38101 tggtggttac gtaaccgcga tcacaacggc aacggcgtgc cggaatatgg cgcgacccgc
    38161 gacaaagccc acaacactga gagcggcgag atgctgttta cggtgaagaa aggcaacaaa
    38221 gaagagacgc agtctgggct gaacaactac gcccgcgtgg tggagaaagg ccagtacgac
    38281 agtctggaaa ttccggcaca ggttgctgca tcgtgggaat cggggcgtga tgatgccgcc
    38341 gtctttgggt ttatcgacaa agaacagctg gataaatatg tcgcaagcgg cggcaaacgt
    38401 agcgactgga cagtgaaatt cgccgaaaac cgcagtcagg acggaacgtt gctggggtac
    38461 tcgctattgc aggagtcggt ggatcaggcc agctatatgt acagcgataa ccattatctg
    38521 gcggagatgg cgacgattct cggtaaaccg gaagaggcta agcgctatcg ccagttggca
    38581 cagcagctcg cggactacat caacacctgt atgttcgacc cggctacgca gtacttctat
    38641 gacgtgcgta ttgaagataa accgctggcg aacggctgcg cgggcaaacc gattgttgag
    38701 cgcggtaaag ggccggaagg ctggtcgccg ctgtttaacg gtgcggcaac gcaggccaat
    38761 gccgacgcgg tggtgaaggt gatgctcgat cctaaagagt tcaatacctt tgttccgctg
    38821 ggaacggcgg cgttaaccaa tccggccttt ggcgctgata tctactggcg cgggcgcgta
    38881 tgggtggatc agttctggtt tggtctgaaa gggatggagc gttacggtta tcgcgatgat
    38941 gccctgaaac tggcggatac gttcttccgg cacgccaaag ggttaaccgc cgatggccca
    39001 attcaggaaa attacaaccc gctgacaggc gcacagcaag gcgcaccaaa tttctcctgg
    39061 agtgccgcac atttttatat gttgtataac gattttttcc gtaagcaata atttgcaacg
    39121 gctggcggat gcggcgtgaa cgccttatcc gccctacata ttcgttcccg taggctggat
    39181 aagacgcgac aagcgtcgca tccggcatct gcacctcgcc accggcggat gctgcgtgaa
    39241 cgccttatcc gccctacatg ttcgttcccg taggctggat aagacgcgac aagcgtcgca
    39301 tccggcatct gcacctcgcc actggcggat gcggcgtgaa cgccttatcc gccctacatg
    39361 ttcgttcccg taggctggat aagacccgac aagcgtcgca tccggcgtct gcaccgcgcc
    39421 actggcggat gcggcgtgaa cgccttatcc gccctacatg tgcaatcccc caggtcggat
    39481 agggcccggc aagcgtcgca tccggcgtta ccaccgggcg tattcttttt gaatcccatc
    39541 acaaaccccg cactcccctt ttcccttttc tccggcgacg gctaaattag aactcatccg
    39601 accacataac aattatttta catactggac aattttatga gctacccgtc gctgttcgcc
    39661 ccgctggatt taggttttac cacgttaaaa aaccgcgtgt tgatgggttc aatgcacacc
    39721 gggctggagg aatacccgga cggtgctgaa cggctggcag cgttttatgc cgaacgcgcc
    39781 cgtcacggcg tggcgctgat tgttagcggc ggcattgcac cagatttaac aggcgttggc
    39841 atggaaggcg gcgcaatgct caatgacgcc agccagatcc cacaccatcg caccattacc
    39901 gaagcggtac atcaggaagg cggcaaaata gccctgcaaa ttttgcatac cgggcgctac
    39961 agctaccaac cgcatctggt cgcaccgtcc gcattgcagg cccccatcaa ccgtttcgtt
    40021 cctcatgaac tcacccatga agagatcctg caactgatcg acgatttcgc ccgctgcgcg
    40081 caactggcac gggaggcagg atacgacggt gtagaggtaa tgggttccga agggtatttg
    40141 atcaacgaat ttctgacgct gcgcaccaat cagcgcagtg accagtgggg cggcgattac
    40201 cacaaccgga tgcggtttgc cgttgaagtg gtgagtgcgg tacgcgaacg cgttggcaac
    40261 gacttcatta ttatctaccg actgtcgatg ctcgacctgg tcgaaggcgg cgggactttt
    40321 gccgaaacgg tagagctggc gcaggccatt gaagcggcgg gcgcgaccat tatcaacacc
    40381 ggcattggct ggcatgaagc gcgtattccg accatcgcca cgcccgtgcc acgcggcgca
    40441 tttagctggg tcacgcgcaa actgaaaggt cacgtctcgc tgccgctggt gaccaccaac
    40501 cggattaacg atccgcaggt tgccgacgat attctctcgc gcggcgatgc cgatatggta
    40561 tcgatggcgc gaccgtttct tgcggatgcg gaactgctgt caaaagcgca atcagggcga
    40621 gccgatgaga tcaacacctg cattggctgc aaccaggcgt gtctcgatca gatcttcgtt
    40681 ggcaaggtca cctcgtgtct ggtgaatcct cgcgcctgcc atgaaaccaa aatgccgatc
    40741 cttcccgcca tgcagaaaaa aaatctggcg gtggtcggtg cgggacctgc tgggctggcg
    40801 tttgccatta acgcggcggc gcgtggtcat caggtaacgt tgtttgacgc gcacagtgag
    40861 atcggcgggc agtttaatat cgccaaacag atccccggca aagaggagtt ttacgaaacg
    40921 ctgcgctatt accgccggat gatcgaagtg acgggcgtga cgctaaaact caatcacacc
    40981 gtgacggcgg atcagttaca ggcgttcgat gaaacgatcc tcgccagtgg gatcgtgccg
    41041 cgcactccgc ccatcgacgg gatcgatcat ccgaaggtat tgagttatct cgatgtactg
    41101 cgcgacaaag cgccggtggg caacaaagtt gccatcatcg gttgtggcgg gattggtttt
    41161 gatacggcga tgtatttaag tcagccgggc gaatccacca gccagaatat cgccgggttc
    41221 tgtaatgaat gggggatcga cagcagccta caacaggctg gtggcttaag cccgcaggga
    41281 atgcagatcc cgcgtagccc acggcagatt gtgatgctcc agcgcaaagc cagcaaaccg
    41341 ggacaggggt taggcaaaac caccggctgg atccatcgca ccaccctgct ctcgcggggt
    41401 gtgaaaatga tcccaggcgt aagttatcag aagattgacg atgacgggct gcatgtggtg
    41461 atcaacggcg aaacgcaggt attagcagtg gacaatgtgg tgatctgcgc agggcaagag
    41521 ccaaaccgcg cattggcgca gccgctgatt gatagcggga aaacggtgca tttaattggc
    41581 ggctgcgatg tggctatgga gctggacgca cgacgggcaa ttgcccaggg aacacggctg
    41641 gcgctggaga tttaaatcgt gcattgaagt gccggatgca acgcatccg
//
LOCUS       DAZFTN010000030        41375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFTN010000030 DAZFTN010000000
VERSION     DAZFTN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(187..834)
                     /gene="ychE"
                     /locus_tag="ACHKV2_001768"
     CDS             complement(187..834)
                     /gene="ychE"
                     /locus_tag="ACHKV2_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO6939667.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(865..918)
                     /gene="ychE"
                     /locus_tag="ACHKV2_001769"
     CDS             complement(865..918)
                     /gene="ychE"
                     /locus_tag="ACHKV2_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO6939668.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1311..3986
                     /gene="adhE"
                     /locus_tag="ACHKV2_001770"
                     /gene_synonym="adhC"
     CDS             1311..3986
                     /gene="adhE"
                     /locus_tag="ACHKV2_001770"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO6939669.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4287..4904)
                     /gene="tdk"
                     /locus_tag="ACHKV2_001771"
     CDS             complement(4287..4904)
                     /gene="tdk"
                     /locus_tag="ACHKV2_001771"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO6939670.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5508..5921
                     /gene="hns"
                     /locus_tag="ACHKV2_001772"
     CDS             5508..5921
                     /gene="hns"
                     /locus_tag="ACHKV2_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO6939671.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6065..6973)
                     /gene="galU"
                     /locus_tag="ACHKV2_001773"
     CDS             complement(6065..6973)
                     /gene="galU"
                     /locus_tag="ACHKV2_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO6939672.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7175..8188)
                     /gene="rssB"
                     /locus_tag="ACHKV2_001774"
     CDS             complement(7175..8188)
                     /gene="rssB"
                     /locus_tag="ACHKV2_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO6939673.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8280..9185)
                     /gene="rssA"
                     /locus_tag="ACHKV2_001775"
     CDS             complement(8280..9185)
                     /gene="rssA"
                     /locus_tag="ACHKV2_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO6939674.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9298..9756
                     /gene="ychJ"
                     /locus_tag="ACHKV2_001776"
     CDS             9298..9756
                     /gene="ychJ"
                     /locus_tag="ACHKV2_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO6939675.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9806..10648
                     /gene="purU"
                     /locus_tag="ACHKV2_001777"
     CDS             9806..10648
                     /gene="purU"
                     /locus_tag="ACHKV2_001777"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO6939676.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10808..10892
                     /locus_tag="ACHKV2_001778"
     tRNA            10808..10892
                     /locus_tag="ACHKV2_001778"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10842..10844,aa:Tyr,seq:gta)
     gene            10937..11067
                     /locus_tag="ACHKV2_001779"
     ncRNA           10937..11067
                     /ncRNA_class="other"
                     /locus_tag="ACHKV2_001779"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11102..11186
                     /locus_tag="ACHKV2_001780"
     tRNA            11102..11186
                     /locus_tag="ACHKV2_001780"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11136..11138,aa:Tyr,seq:gta)
     gene            11233..11364
                     /locus_tag="ACHKV2_001781"
     ncRNA           11233..11364
                     /ncRNA_class="other"
                     /locus_tag="ACHKV2_001781"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11411..11542
                     /locus_tag="ACHKV2_001782"
     ncRNA           11411..11542
                     /ncRNA_class="other"
                     /locus_tag="ACHKV2_001782"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11726..12403)
                     /gene="narI"
                     /locus_tag="ACHKV2_001783"
     CDS             complement(11726..12403)
                     /gene="narI"
                     /locus_tag="ACHKV2_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6939677.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12403..13113)
                     /gene="narJ"
                     /locus_tag="ACHKV2_001784"
     CDS             complement(12403..13113)
                     /gene="narJ"
                     /locus_tag="ACHKV2_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6939678.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13110..14648)
                     /gene="narH"
                     /locus_tag="ACHKV2_001785"
     CDS             complement(13110..14648)
                     /gene="narH"
                     /locus_tag="ACHKV2_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6939679.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14645..18388)
                     /gene="narG"
                     /locus_tag="ACHKV2_001786"
     CDS             complement(14645..18388)
                     /gene="narG"
                     /locus_tag="ACHKV2_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO6939680.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(18781..20172)
                     /gene="narK"
                     /locus_tag="ACHKV2_001787"
     CDS             complement(18781..20172)
                     /gene="narK"
                     /locus_tag="ACHKV2_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO6939681.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            20511..22307
                     /gene="narX"
                     /locus_tag="ACHKV2_001788"
     CDS             20511..22307
                     /gene="narX"
                     /locus_tag="ACHKV2_001788"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO6939682.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22300..22950
                     /gene="narL"
                     /locus_tag="ACHKV2_001789"
     CDS             22300..22950
                     /gene="narL"
                     /locus_tag="ACHKV2_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO6939683.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(22951..24345)
                     /gene="ychO"
                     /locus_tag="ACHKV2_001790"
                     /gene_synonym="iatA"
     CDS             complement(22951..24345)
                     /gene="ychO"
                     /locus_tag="ACHKV2_001790"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO6939684.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            24531..24884
                     /gene="ychN"
                     /locus_tag="ACHKV2_001791"
     CDS             24531..24884
                     /gene="ychN"
                     /locus_tag="ACHKV2_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO6939685.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(24928..25623)
                     /gene="chaC"
                     /locus_tag="ACHKV2_001792"
     CDS             complement(24928..25623)
                     /gene="chaC"
                     /locus_tag="ACHKV2_001792"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO6939686.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(25784..26014)
                     /gene="chaB"
                     /locus_tag="ACHKV2_001793"
     CDS             complement(25784..26014)
                     /gene="chaB"
                     /locus_tag="ACHKV2_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO6939687.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26284..27384
                     /gene="chaA"
                     /locus_tag="ACHKV2_001794"
     CDS             26284..27384
                     /gene="chaA"
                     /locus_tag="ACHKV2_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO6939688.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            27783..27890
                     /locus_tag="ACHKV2_001795"
     CDS             27783..27890
                     /locus_tag="ACHKV2_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6939689.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            28319..28426
                     /locus_tag="ACHKV2_001796"
     CDS             28319..28426
                     /locus_tag="ACHKV2_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6939690.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(28574..29428)
                     /gene="kdsA"
                     /locus_tag="ACHKV2_001797"
     CDS             complement(28574..29428)
                     /gene="kdsA"
                     /locus_tag="ACHKV2_001797"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO6939691.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(29464..30273)
                     /gene="sirB1"
                     /locus_tag="ACHKV2_001798"
     CDS             complement(29464..30273)
                     /gene="sirB1"
                     /locus_tag="ACHKV2_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO6939692.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(30277..30669)
                     /gene="sirB2"
                     /locus_tag="ACHKV2_001799"
     CDS             complement(30277..30669)
                     /gene="sirB2"
                     /locus_tag="ACHKV2_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO6939693.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            complement(30666..31499)
                     /gene="prmC"
                     /locus_tag="ACHKV2_001800"
     CDS             complement(30666..31499)
                     /gene="prmC"
                     /locus_tag="ACHKV2_001800"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6939694.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(31499..32581)
                     /gene="prfA"
                     /locus_tag="ACHKV2_001801"
     CDS             complement(31499..32581)
                     /gene="prfA"
                     /locus_tag="ACHKV2_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO6939695.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(32623..33879)
                     /gene="hemA"
                     /locus_tag="ACHKV2_001802"
     CDS             complement(32623..33879)
                     /gene="hemA"
                     /locus_tag="ACHKV2_001802"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO6939696.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34093..34716
                     /gene="lolB"
                     /locus_tag="ACHKV2_001803"
     CDS             34093..34716
                     /gene="lolB"
                     /locus_tag="ACHKV2_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO6939697.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            34716..35567
                     /gene="ispE"
                     /locus_tag="ACHKV2_001804"
     CDS             34716..35567
                     /gene="ispE"
                     /locus_tag="ACHKV2_001804"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO6939698.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            35718..36665
                     /gene="prs"
                     /locus_tag="ACHKV2_001805"
     CDS             35718..36665
                     /gene="prs"
                     /locus_tag="ACHKV2_001805"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO6939699.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            36790..38469
                     /gene="dauA"
                     /locus_tag="ACHKV2_001806"
     CDS             36790..38469
                     /gene="dauA"
                     /locus_tag="ACHKV2_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO6939700.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(38524..38802)
                     /gene="ychH"
                     /locus_tag="ACHKV2_001807"
     CDS             complement(38524..38802)
                     /gene="ychH"
                     /locus_tag="ACHKV2_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO6939701.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            39080..39664
                     /gene="pth"
                     /locus_tag="ACHKV2_001808"
     CDS             39080..39664
                     /gene="pth"
                     /locus_tag="ACHKV2_001808"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO6939702.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            39781..40872
                     /gene="ychF"
                     /locus_tag="ACHKV2_001809"
     CDS             39781..40872
                     /gene="ychF"
                     /locus_tag="ACHKV2_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO6939703.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT        10333 a        10742 c        10123 g        10177 t
ORIGIN      
        1 aggattatga tgtggcgaaa ataacatgat gatgaggaga agcagttaag gaaatcctta
       61 taactaactg taatttgaga agaatatttt tacctattcc ttttgctttt gataactgcg
      121 cgggaacaaa gtaaaaataa tgacgaagta aacggagcat ggcagctccg tttcatttga
      181 aaggaattaa ttaagcaggc cggggaaaat ccccttaata ccagtaacga taaattcaat
      241 ccccaatgcc atcagcaata gccccataat acgcgtaatc acgttgatgc cggtctggcg
      301 taaaacccgt accagccacg gtgccatgcg gaacaatccc caacaacata aagcgaacaa
      361 tgcaatagcc acaaagaaac caaacagata gctaatgctg tgataacgcg taccccagac
      421 gatggtagaa ctgatcgccc ctggccccgc catcaacggc aacgccaatg gcaccacacc
      481 aatactttca cgtaccgcgg tttctgattt ttcttgcttg ttctgtttat cctcgccaag
      541 cttgccgctg atcatcgaca tcgctatcgt caccaccagg atgcccccgg cgatacggaa
      601 cgaatcaatt gatataccaa aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca
      661 caagataatg gccacagaca ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt
      721 ctgataactg gtcatgctga taaagacggg aataatccct accgggttga ccagcgcaaa
      781 taacccgatg aaaaatttga agtaaacggg aaaatcaaaa agggtctgaa tcacggttag
      841 ctccgaagca aaagccggat aatgttagcc ataaataagg ttgaaaagac gcgctgacaa
      901 tacgcctttt gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt
      961 gttatctagt tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac
     1021 tgttatctat aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aatttgattt
     1081 ggatcacgta atcagtaccc agaagtgagt aatcttgctt acgccacttg gaagtgacgc
     1141 attagagata ataactctaa tgtttaaact cttttagtaa atcacagtga gtgtgtgcgc
     1201 gagtaagctt ttgattttca tcggttaagc aaatcatcac cgcactgact atactctcgt
     1261 attcgagcag atgatttact aaaaaagttt aacattatca ggagagcatt atggctgtta
     1321 ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg
     1381 ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag
     1441 atgctcgaat cccactcgcg aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag
     1501 ataaagtgat caaaaaccac tttgcttctg aatatatcta caacgcctat aaagatgaaa
     1561 aaacctgtgg tgttctgtct gaagacgaca cttttggtac catcactatc gctgaaccaa
     1621 tcggtattat ttgcggtatc gttccgacca ctaacccgac ttcaacagct atcttcaaat
     1681 cgctgatcag tctgaagacc cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag
     1741 atgcgaccaa caaagcggct gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga
     1801 aagatctgat cggctggatc gatcaacctt ctgttgaact gtctaacgca ctgatgcacc
     1861 acccagacat caacctgatc ctcgcgactg gtggtccggg catggttaaa gccgcataca
     1921 gctccggtaa accagctatc ggcgtaggcg cgggcaacac tccggttgtt atcgatgaaa
     1981 ctgctgatat caaacgtgca gttgcatctg tactgatgtc caaaaccttc gacaacggcg
     2041 taatctgtgc ttctgaacag tctgttgttg tcgttgactc tgtttatgac gcagtacgtg
     2101 aacgtttcgc aacccacggc ggctatctgt tgcagggtaa agagctgaaa gctgttcagg
     2161 acgttatcct gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca gcctataaaa
     2221 ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc ggtgaagtga
     2281 ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact ctggcaatgt
     2341 accgcgctaa agatttcgaa gacgcggtag aaaaagcaga gaaactggtt gctatgggtg
     2401 gtatcggtca tacctcttgc ctgtacactg accaggataa ccaaccggct cgcgtttctt
     2461 acttcggtca gaaaatgaaa acggctcgta tcctgattaa caccccggct tctcagggtg
     2521 gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt tgtggttcct
     2581 ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg
     2641 ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc tacttccgcc
     2701 gtggctccct gccaatcgcg ctggatgaag tgattactga tggccacaaa cgtgcgctca
     2761 tcgtgactga ccgcttcctg ttcaacaatg gttatgctga tcagatcact tccgtactga
     2821 aagcagcagg cgttgaaact gaagtcttct tcgaagtaga agcggacccg accctgagca
     2881 tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg
     2941 gtggtggttc cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa catccggaaa
     3001 ctcacttcga agagctggcg ctgcgcttta tggatatccg taaacgtatc tacaagttcc
     3061 cgaaaatggg cgtgaaagcg aaaatgattg ctgtcaccac cacttctggt acaggttctg
     3121 aagtcactcc gtttgcggtt gtaactgacg acgctactgg tcagaaatac ccgctggcgg
     3181 actatgcgct gaccccggat atggcgattg tcgacgccaa cctggttatg gacatgccga
     3241 agtccctgtg tgctttcggt ggtctggacg cagtaactca cgccatggaa gcttatgttt
     3301 ctgtactggc atctgagttc tctgatggtc aggctctgca ggcactgaaa ctgctgaaag
     3361 aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc
     3421 acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt gtatgtcact
     3481 caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca aacgccctgc
     3541 tgatttgtaa cgttattcgc tacaatgcga acgacaaccc gaccaagcag actgcattca
     3601 gccagtatga ccgtccgcag gctcgccgtc gttatgctga aattgccgac cacctgggtc
     3661 tgagcgcacc gggcgaccgt actgctgcta agatcgagaa actgctggca tggctggaaa
     3721 cgctgaaggc tgaactgggt attccgaaat ctatccgtga agctggcgtt caggaagcag
     3781 acttcctggc gaacgtggat aaactgtctg aagatgcatt cgatgaccag tgcactggcg
     3841 ctaacccgcg ttacccgctg atctccgagc tgaaacagat tctgctggat acctactacg
     3901 gtcgtgatta tgtagaaggt gaaactgcag cgaaaaaaga agccgctccg gctaaagctg
     3961 agaaaaaagc gaaaaaatcc gcttaatcag tagcgctgtc tggcaacata aacggcccct
     4021 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
     4081 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
     4141 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaactgccga
     4201 gaagggtgta tagcccggaa gaagtgcgta aaacgaactg acaggataaa agtgccctgc
     4261 tcaccctgtc agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg aatagccgtt
     4321 aatgagccga cttgtaacgc ctctttatag tgtttacggc atacagaaac gtatcgttca
     4381 ttaccaccaa taaccacctg ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc
     4441 agcaccatgc ttgctttacg gccacaaaaa cagatggttt ttaattcaac cagtttgtcg
     4501 gaccatgcca gtaagtattg gctgccaata aataattcac ctcgaaaatc ggtacgtaaa
     4561 ccataacaaa gtacgggtat atcgagttga tcgacaacct ccgataattc atatacttgt
     4621 tgtctggtta aaaactggca ttcatcaacc agtacgcaat gaattgcctg ctgctcatgt
     4681 tccgcacgaa tctcatcaaa taatgatgaa ttttggttaa ataattttgc aggcgatgac
     4741 aaacctatac gcgaactgac tttcccggca ccaaagcgat catcaatttc tgccgtatat
     4801 acgacagtgc gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac
     4861 ttacccgcat tcattgcgga atagtagaaa tatagctgtg ccatcagcca caggccctca
     4921 atgatatgaa ataaagttgg ctggagttta tcataattcg tcagtttttc agctcaacag
     4981 tatgcacaga agtggtatat gcgttctccc ttacgaagcc ttgcataatc cttctgagct
     5041 atcattacaa ctgcctcgct tgttataagc gggtaaatga ctgctggtaa ctattcacaa
     5101 tctttaacct gttgcgcaag taatagccct ctgttgacct ccaggagata gtgcaatact
     5161 aagtccctgc tcttattgcg actgttctac ttttcatcat tcgcttaata gggaattctc
     5221 gtaaacacga ctaatacaga agactgaaag gtcgtcagcc tacgataatc tccccataaa
     5281 atgtgacatg aatcaggaag ttttaacctc acgtgctgcg aaatcatcgg tacaaatagg
     5341 gctatatgcc gcgtcttttc tggctaattt tatgaaaaga tatttattgg cggcacaaaa
     5401 taaagaacaa ttttgaattc cttacattcc tggctattgc acaactgaat ttaaggctct
     5461 attattacct caacaaacca ccccaatata agtttgagat tactacaatg agcgaagcac
     5521 ttaaaattct gaacaacatc cgtactcttc gtgcgcaggc aagagaatgt acacttgaaa
     5581 cgctggaaga aatgctggaa aaattagaag ttgttgttaa cgaacgtcgc gaagaagaaa
     5641 gcgcggctgc tgctgaagtt gaagagcgca ctcgtaaact gcagcaatat cgcgaaatgc
     5701 tgatcgctga cggtattgac ccgaacgagc tgctgaatag ccttgccgcc gttaaatctg
     5761 gcaccaaagc taaacgtgct cagcgtccgg caaaatatag ctacgttgac gaaaacggcg
     5821 aaactaaaac ctggactggc cagggccgta ctccagctgt aatcaaaaaa gcaatggatg
     5881 agcaaggtaa atccctcgac gatttcctga tcaagcaata atcttttgta gattgcactt
     5941 gcttaaaatc ccgccatcgg cgggattttt tattgtccgg tttaagacaa tttaataaga
     6001 ataagctata aaaaaacggc gtcgattgct caacgccgtt tcgtggataa caccgatacg
     6061 gatgttactt cttaatgccc atctcttctt caagccaggc tttaaattcc gtgccaaggg
     6121 tgttatgacg aataccgtat tcaacgaagg cctgcatgta acctaattta ttaccgcagt
     6181 catggctctt ccctttcata tgataggctt caaccgtttc tttttcgatt agcatatcaa
     6241 ttgcgtcggt gagctgaatt tcatcaccag ctcccggagg ggtttttgcc agcaacggcc
     6301 aaatatccgc actaagtacg taacgaccca caatagcgag attagacggc gcaacatccg
     6361 cttttggttt ttcaaccacg ccaaccatcg gtacgctttc acccggcgct aattcaacgc
     6421 ctttgcaatc cacaacgcca tatgcggtca catcagcaac cggttcaacc atgatctggc
     6481 tatgacccgt ttcatcgaag cggcggatca tctctgccag gttatcctgt gacaaatcgg
     6541 attcatattc atccagaata acgtcaggca aaataacagc cacaggttca tcacccacta
     6601 ccgggtgagc acacaatacc gcgtgtccca ggcctttcgc cagcccctga cgaacttgca
     6661 taatagttac gtgcggcgga caaatagact gcacttcatc aagcagttga cgttttacac
     6721 gtttttccag cattgcttcc agttcaaaac tggtatcaaa gtggttttca atagagtttt
     6781 tagatgagtg tgtaaccagc acaatttcag taatgccagc cgcaatacat tcattcacga
     6841 cgtattgaat taatggctta tcgacaagtg gcagcatctc tttcgggatg gctttcgtcg
     6901 ccggcaacat cctggttcct aatcccgcaa cggggataac ggcttttttg actttcgtat
     6961 taatggcagc catttaaatt ctcctggact gttcgtgttt tgaacgtgtt catatttctg
     7021 tatcgcatcc cagtatatca gcaccccgga ggggctaagt tctgaaaagg atgcgagtta
     7081 gcataattaa gacaattact tatacgaatg gcgacgatag taccaccagc cgacattagc
     7141 tggtaatgca aatttagccc gcgttatcgt ttgctcattc tgcagacaac atcaagcgca
     7201 gtcgaccacc ggttccccat atttggcatt gccaggcatc gcatcgctgg ctcaattgat
     7261 tcaaataagc gttacctaaa gtgcctaacg gaacaccatt actgatttgc acctggtgtt
     7321 cgccggtatt taacgtcgca ttcagacccg cagaaaccag aatgagattt ttcagttcgc
     7381 gatgataata gccaactaat agcggaaact gccccggcag attggcctga cggagtaaat
     7441 ggtttacctg cttcaataac gcgcccaact ctggcaaccg ttgattttgg tgtgcaagct
     7501 gttcctgtaa taatccgtta aacaatgcgc gtaataacaa ggcagcaagt acgccattat
     7561 gtccagctcg ggttacatca aggcaataaa atgccagatc gttttccgaa agtgcggcaa
     7621 tatcaagcac caggccgggt ttgtccgcgg caaccaattg acggtaatta acccggcaat
     7681 gggaaatcac ctgctgaacc ggcggttgta gttcctgtaa taatttcgcc gctgcggcag
     7741 ggttatcaac cattgcatcc cagtcgcgaa aaagcctttc ctcttcctca acgcgcgaat
     7801 taaacatgct gggatagaga caggcaaaaa ccatctcgcg caagcgattc agatctttaa
     7861 ctggtttcag caaaacatct tcaacgccca gacgtaacgc tttggcaata tctgccatat
     7921 tttcagtggc agatatcacc agaactgggg tctggtcgcc tctgttacgt atatgctcca
     7981 gcagtttaag cccgttcatt cgtggcatcg cgatatcaca tatcatcagg tctggagtga
     8041 aacctcccag caactcaagg gcatccaccc catcagccgc cagtaccgtt gtcgctccca
     8101 atgaggaaaa ccacgaatcc agaagcgagc gaaatacctg ctcatcttca acaatgagaa
     8161 tctgttttcc gaccaatggc tgcgtcatgt tctctcccct gactggcttt actcaatagt
     8221 ggcatgctat tgccacctgc gcctgtcaga attaacttaa gtgtagataa aaattctggt
     8281 caaatgttgg tgcgtaccaa cggcaaaagt tcgtccattt tcttttccac tgccagctgc
     8341 ccggccgcaa tggcagcgtg cgcgcgatgg aaatcaagcg tagatatttg cgggcaaaca
     8401 ggttgaatca gaatatcggg cggatcacct gccatgcggt tccttttaag gcggttctcc
     8461 agcacctgga tagaagtggt cataatctct gttgccgttg gtgctgtcac cgcccgacgt
     8521 gtcgttatac tgcctaacct ttctttcaga cgcgcatgcc acggcagaga atcaccattc
     8581 tcgctatttt cttcactgac attaaaggag agcaaatctt gttgcatcaa atgagcatcg
     8641 tgctgcaggt cgaccgctat cacaatatca gcacccaatg cacgcgtgag cgaaatagga
     8701 attgggttaa cgactgcccc atcaaccagc cagtagccgt tatgcgcaac gggtgccatt
     8761 agtcctggaa tactgcatga tgcgcgaata gcaagatgga gatcgccttc agtaaaccat
     8821 aattcacgcc cagtacttaa attggtggca acagccgcga agcgacggga acaattttcg
     8881 atctctgttt ccggcattat ttcgcggtat tgattgaaga cacgctcgcc gcgcagtaac
     8941 ccaccgcgct gccaggagag atccatcagt cgtaaaacat cccaataact gaaagaggtc
     9001 acccaatctt ccagcgcaga taatcgatcg catgcatagg ccgcgcccac cagcgaacca
     9061 attgaacatc ctgcaacgat atcaatttca atacccactt tttttagcgc attaataacg
     9121 ccaatatgcg accaacctct cgccgcgcca gatcccagcg ccagccctat ttttatcttt
     9181 ctcattattc ccgcaagatt cccctggaat gcaatcgtag ccacatcgct actgctcggt
     9241 taacatagtg ctactcaggc ggttaatacg ccgttattgt ttccagggag attatttgtg
     9301 tctcagcttt gtccctgtgg tagtgctgtc gagtatagcc tatgttgcca cccttatgtg
     9361 tctggtgaaa aggttgcacc tgatccagaa catctcatgc gttcgcgtta ctgcgctttt
     9421 gtgatgcaag acgcagatta tttaataaag acctggcatc catcttgtgg agcagcggcg
     9481 ttacgtgccg aattgattgc cggatttgcg catactgaat ggttgggatt gaccgtattt
     9541 gaacattgtt ggcaggacgc cgacaatatc ggatttgtca gttttgttgc tcgatttacc
     9601 gaaggtggca aaacaggtgc aataattgaa cgttcgcgat ttttgaaaga aaacggtcaa
     9661 tggtactata ttgacggtac acgtccgcag tttggccgca acgatccctg cccttgtggt
     9721 tcaggtaaaa aatttaaaaa gtgctgcggc caataatggt tgacggtacg gtttagcaaa
     9781 cactctcaac aaggtttttc cagcaatgca ttcactccaa cgtaaagttc tgcgtactat
     9841 ttgtccggac caaaaaggtc tgatcgcacg tattaccaat atttgctaca agcacgagtt
     9901 aaatatcgta cagaacaatg aatttgttga tcaccgtacc gggcgctttt ttatgcgcac
     9961 ggaactggaa gggattttta atgattccac cctgctggcg gatctcgata gcgcattgcc
    10021 agaaggctcc gtgcgtgagc tgaatcctgc cggtcgtcgc cggatagtga ttctggtcac
    10081 taaagaagcg cattgccttg gcgatttgtt gatgaaagcc aattatggcg gcctggatgt
    10141 cgaaatcgcg gcagtgattg gtaaccacga tactttacgt tctctggttg agcgttttga
    10201 tataccgttt gagctggtaa gccatgaagg gttaagccgc aacgagcacg atcaaaagat
    10261 ggcggatgcc attgatgctt atcaacctga ctacgtggtg ctggcgaagt atatgcgggt
    10321 attaacaccg gaatttgtgt cacgcttccc gaataagatc atcaatattc accattcctt
    10381 cctgccagcg tttatcggcg cacgtcctta tcaccaggcc tatgaacgtg gcgtgaagat
    10441 tattggcgca accgctcact atgtgaatga caatctggac gaaggcccaa tcatcatgca
    10501 ggacgttatt catgtcgatc atacctacac agctgaagat atgatgcgcg caggtcgtga
    10561 cgtcgagaaa aacgtcttaa gtcgcgcgct ctacaaagta ctggcgcagc gcgtctttgt
    10621 ttacggtaat cggacgatta ttctttaatc gccagcaaaa ataactggtt acctttaatc
    10681 cgttacggat gaaaattacg caaccagttc atttttctca acgtaacact ttacagcggc
    10741 gcgtcatttg atatgatgcg ccccgcttcc cgataaggga gcaggccagt aaaaagcatt
    10801 accccgtggt ggggttcccg agcggccaaa gggagcagac tgtaaatctg ccgtcatcga
    10861 cttcgaaggt tcgaatcctt cccccaccac catgattcac cacagcgatg tggtaacttc
    10921 ggtgagattg tgaaagtttg cactcatcgg ggaagggtga gaaccttcga ctaaggttcg
    10981 actcgagcga aagcgagaga acgttttcac aggcaacggc ccgaagggtg aggagcgaag
    11041 cgacgaataa tcctttcccc accgccatct caagcattac ctcaaaattt gaattacccc
    11101 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
    11161 aggttcgaat ccttccccca ccaccatcac tttcaaaagt ccctgaactc tcaagcgaat
    11221 ccgtaaataa atattctgcc caagcggcga aggatgagaa gcttcgacta aggttcgact
    11281 cgagcgccag cgagagagcg ttgccgcagg caacgacctg aagggcgaag cgcgcagcac
    11341 tgagtaatcc ttcccccacc accatcactt tcaaaagtcc ctgaactctc aaacgaatcc
    11401 gtaattaaat attctgccca agcggggaag gatgagaagc ttcgaccaag gttcgacttg
    11461 agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg
    11521 agtaatcctt cccccaccac catcgcttcc tgaaataatc aaaacctgtt actccgagct
    11581 aattttgccc aatggcacca cgtttaccgg acctacaata tcctcccttg taggtcggat
    11641 aagcgaagcg catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc
    11701 agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg tacttacgtg
    11761 tcagatactc caccggtacg ctccagatgt gtaccagacg cgagaacggg aacagcagga
    11821 ataacgtcat ccccagcacc aggtgtagac ggaagataaa cgccacacca tcgaggtgtt
    11881 gagaagcgcc accgtggaag gtcactaccg actgcgccca gccaaccagt ttcatcatct
    11941 cgctaccgtc catatgttgg gcggagaacg gaatggtcaa caggcccagc gcgcactgga
    12001 taacgagcag cgacaggatc agtatatccg ctccggtagt cgttgcacgc acgcgtgggc
    12061 tgaacagacg acgtttaagc aaaagcacgc cgccaatcag acacagcacg ccgctggcac
    12121 caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc
    12181 agtgcggcgt caacataccg aagaagtgac cgacaaaaat ccccagaata ccgatatgga
    12241 acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc
    12301 aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta
    12361 tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt
    12421 ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat
    12481 tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccaaacagc
    12541 atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca attttttccg ccactttgtc
    12601 gctgtcgata gcggtattgg ccaatttcag cagcagatca aacagcacgg cataacggct
    12661 ttcacgctgt tgcagacgcg cactcagcaa tgccagaatc ggcgcgatat ctttcaaacc
    12721 ttccacggct tcgctttgcg gcagctgcgc caggtactcc agatacagcg gcagatgatc
    12781 cggcaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac
    12841 catcgcctgg ccgcggtcgc gggattcgcc gtgcacatgt tcaaacagca acagtgacgt
    12901 ggcgcggcca cggtcgaaca gttcgctgta ctgcgcctgg gcatcgagcg gatccatcgt
    12961 cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatcc tcttttggca gatttttcga
    13021 cgcggcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat ccggatattc
    13081 aaggagacgc gatacaatca cgagttcgat catggatgcg gctccgtttt gctggtcaca
    13141 tcgatggcat cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag
    13201 ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca
    13261 cgatgactac tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac
    13321 atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcaacttta
    13381 ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagcgcgcgc
    13441 agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc
    13501 aggctttcga cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgctgcagac
    13561 tgaatcggag agagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc
    13621 agcggcagcg ccagtttcca ttccattgcc attttataaa ccggcgactg ctgtgcagct
    13681 tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc
    13741 ggatcgagga acacctccag ctgacgctgg tagagatctt tctcgttctc ggtgctggct
    13801 gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa gataacggat acgaccgaca
    13861 caggtttctg agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac
    13921 ttctcagact taccgctctt ccagttgaag tagatttttt tgtacgggca tccggtgatg
    13981 cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc
    14041 ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac
    14101 aggcgcggca aatacatcat gaaggtgttt tcgaactggc tatacatcgc cttctggatg
    14161 ttgtcgaagt tcttgtcttt cgccagtttg tcaaactcac cgcccaggtc atcttcccag
    14221 ttcggccctt tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca
    14281 atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc aaaatcgaac
    14341 ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc
    14401 atggcacggt tacccatgcg cggttgcagt ttgccgttga ttttacggat ccagccgcct
    14461 ttgtattttt cctggttttc ccagtcagtc gggaagccct ggcccggctt ggtttccacg
    14521 ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact
    14581 gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga
    14641 attttcattt tacgctctcc tgtacctgat cattgccttc gccatctaac cagtcaatgt
    14701 tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt
    14761 taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac
    14821 gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa
    14881 cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa
    14941 ccgcacgggc agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga
    15001 tacccagatc tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag
    15061 tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca
    15121 ggaagttgag cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg
    15181 aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact
    15241 ggtgatcctg atacagttgc tgacgaccag agagcgtacg ccatgggatc agctcgtgaa
    15301 cgttggtgta accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc
    15361 tggagataat tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt
    15421 tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca gcccaggctt
    15481 tcacggctac ctgaccgttg gtttccggtg ccagtgtcag gatcatctct gccgcatcaa
    15541 ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct
    15601 tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccgttaccga
    15661 ttttctccat cagcgggccg atagaggtaa agcgttcgta agttgccgga taatcgcgct
    15721 ctacgaccat aatgtgcggt gcagttttgc ctgggatcag gtcgcattcg ccttttttcc
    15781 agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcgtgctgg ataggcagcg
    15841 tgacgatgtc ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga
    15901 tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca
    15961 gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg
    16021 ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa
    16081 ccaccagatc cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca
    16141 cgccgccctg ctgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact
    16201 tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagataaaca
    16261 ggttacgcgg gtggtttttg ccgttttccg gctgttccgc agcgaaacgg atagaaccct
    16321 ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttct gcttcgccag
    16381 cgatagtcag cgggttagtg cctaactgcg gcgcagacgg cagccagccc atgcgttctg
    16441 cacggacgtt aaagtcgatc agatgtccgg tatagcggga tttatccgcc atcggcgaca
    16501 gcagttcttc cgcagtcacg gtttcataac gccactggct ggagtggtta tagaaataag
    16561 aggtgctgtt catgtgacgc gccggacgct gccagtcgag ggcaaacgcc agcggctgcc
    16621 agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct
    16681 gaccgacaca gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt
    16741 gataccagtg gttcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag
    16801 cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct
    16861 gctcggccca ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt
    16921 cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag atcataaacg gtggtcacca
    16981 gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt
    17041 tttccagttc cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta
    17101 cctcagcgat ctcatcctgg ctacccagca ggctcagttg cagctcggtt tcttcgccag
    17161 ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag
    17221 aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat
    17281 tgttttcctg gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt
    17341 agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat
    17401 agtcggtgaa atactggctt gggttgtcga gatggaattc acgcagcatt acgtggccca
    17461 tcgccagcgc cattgccgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt
    17521 tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggtgcctttg taacgcactt
    17581 cagtaaagaa gtgagcatct ggggtacgcg tctgcggcac gtttgaaccc caggcgatga
    17641 tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt
    17701 gcggagacgc aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa
    17761 tcagcgagag atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg
    17821 agaaaccagc aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga
    17881 tcagttcgtt cacctcctgc caggaagaac gaacaaaacc accgcgtcca cgcgcctgct
    17941 taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc
    18001 tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt
    18061 tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag
    18121 gttcatggtt tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg
    18181 tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt
    18241 gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac
    18301 ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt
    18361 agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc
    18421 tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat
    18481 tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctaaga taccccttta
    18541 ggataattaa cagttacagc ataacatgct gtaaattaag acgttttata cctgtagtta
    18601 tttaaactat taagattttt atgcaaatgg gtattaagga gtattcccca tgggtaacat
    18661 attaatggtt cttaatgaaa tccggcgaaa taaaccatat aacattagta tgttgtaact
    18721 aaatgacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa atattaagat
    18781 ttatttttta gaatgccgac catataccgc ccaggtaatc actacgcagg cgatatagaa
    18841 aatcaaaaat actttcattg cgccgactgg cgaacccgtt aatgccagcg agctaccgaa
    18901 cgctttcggg ataaagaagc caccaatcgc gccaatcgca gagatgaaac ccaacgccgc
    18961 cgccgtgtcg gttgccgctt cacgcatcgc acgttcgtca gaaccccctt ctgctttcac
    19021 gcgatccatt gtcagtttac ggaagatcac ggaaatcatc tggaaagtgg aaccactacc
    19081 cagcccagct gtcaggaaca gcgccaggaa gaccgcgaag aacgccatga agcttccgcc
    19141 ctgcccgtca gtcggtaagg tcaggaacag caggccgctg aaaatcgcca tcagaataaa
    19201 gttcaccagc gtgacacgag ttccgcccag acggtcagat aatgcaccac ctgcagaacg
    19261 cgccagcgca ccaataaacg gcccgaagaa agcgtattgc agaatctgaa catccgggaa
    19321 ctgcgttttt gacagcatcg caaagcccgc ggagaagccg atgaaggaac cgaaggttgc
    19381 cagatacagc aggctcataa tccacagatg accccgtttg agtaccggca actgctcctt
    19441 gatggaggct ttcgaggtag caagatcgtt catgccaaac cacgccgcaa tggtgaagat
    19501 ggcaaggaac ggcacccata tccaggacgc attcgccaga tacagctcag tcccatccgg
    19561 ctgtttgacg ccctggctac caaatactgc gaaaatcgac agtgatacca ccagcggagc
    19621 aaccaactgc ataacgctaa cgcccatgtt gcccagacca ccattcaggc ccagcgcgcc
    19681 accctgcttc tgtttcggaa agaagaagct gatgtttgcc atactggatg cgaagttcgc
    19741 gccagcaaag ccgcacagca gagagatgat gatgaagacg ctataaggcg tggaggtatc
    19801 ctgcacggca aaacccagcc agacacaagg aataatcaga ataccggtgc tgaacgccgt
    19861 ccagcgacga ccaccgaaga taggaaccat aaaggagtat ggaacacgta ataacgcgcc
    19921 agaaaccgaa ggcaacgcag tcaacataaa tagctgatcg gtcgtaaaat taaaaccgac
    19981 tttcggtaag ttcaccgcaa cagcgctgaa caacatccat acgcaaaacg ccagcagcag
    20041 acagggaacg gaaatccaca ggttgcggct ggcaatacgt tgaccgcgtt gttgccagaa
    20101 cgcaggatct tccggtcgcc aatctgtaat gacagctcca gtagcccttt cgggggcgga
    20161 tgagtgactc atagacacct ctgatactcg tttcgatgtc tgccacctta gtgtctgtag
    20221 ctaaaggcaa tttgatgtaa atcaaacgat aagataactt tatctatcat tgatatttat
    20281 cattacccat agtgagtaca gtgatttcat aaaaattatg agatttttca cggtgctgta
    20341 aaatccctac ccttaccgat gtaaagctac taaccacacg gcaaataagg agtaactctt
    20401 tccgggtatg ggtatacttc agccaatagc cgagaatact gccattccag aatgtatcgt
    20461 cacattcatt aaggttattg ctcatttaaa gcctgaagga agaggtttac atgcttaaac
    20521 gttgtctctc tccgctcacc ctggttaatc aggttgcgct tattgtgttg ctttctactg
    20581 ctattggact ggcagggatg gcggtttctg gctggctggt gcaaggcgtt cagggcagcg
    20641 cccatgcgat caacaaagcg ggatcgctgc gcatgcaaag ttaccgtctg ttggcagcag
    20701 tgccattaag cgagaaagac aagcccttaa ttaaagagat ggaacaaacg gcatttagcg
    20761 ccgagttgac tcgagcagca gaacgagacg gacaactggc gcaattacag ggtttacaag
    20821 attactggcg taatgaactc atccctgcac tgatgcgtgc acaaaaccgc gaaacggtgt
    20881 cagcggatgt cagccagttt gttgccgggc ttgatcagct ggtatctggt tttgaccgca
    20941 ccacggaaat gcgcattgag acagtggtac tggtccatcg ggtaatggcg gtatttatgg
    21001 cacttttact ggtgttcact attatctggt tgcgggcgcg actgctacaa ccgtggcggc
    21061 aactgctggc aatggcgagt gccgtcagcc atcgcgattt tacccaacgc gcaaacatca
    21121 gcgggcgcaa cgaaatggcg atgcttggaa ctgcgttgaa caatatgtct gcagaactgg
    21181 ccgaaagtta tgccgtactt gagcagcggg ttcaggagaa aaccgccggg ctggagcata
    21241 aaaatcagat cctctctttt ttatggcagg ctaaccgccg tttgcattcc cgcgccccgc
    21301 tgtgtgaacg cctgtcacct gtactcaacg gcttacagaa tttaaccctg ctacgtgata
    21361 tcgaactgcg ggtgtatgac actgatgatg aagagaatca tcaggagttt acctgccagc
    21421 cagatatgac ttgtgatgat aaaggctgcc agctctgccc gcgcggcgta ttacccgttg
    21481 gtgatcgcgg cacgaccctg aagtggcggc tggctgactc tcatacgcag tacggtattt
    21541 tgctggcgac cctaccgcag gggcgtcatc ttagccatga tcaacaacaa ctggtggata
    21601 ccctggttga acaactcacc gccacgctgg cgctggatcg gcatcaggaa cgtcagcaac
    21661 agttgatcgt gatggaagag cgtgccacca ttgcgcgcga actgcatgat tctattgccc
    21721 aatctctctc ttgcatgaag atgcaggtga gttgtttaca gatgcagggc gatgcgctgc
    21781 cagaaagcag ccgcgaactg ttaagtcaga tccgtaacga actgaatgca tcctgggcgc
    21841 agttgcgtga attgctcacc acattccgct tgcagctcac cgagcctgga ttacgtccgg
    21901 cgctggaggc gagttgcgaa gagtacagcg ccaaatttgg cttcccggtg aagctggatt
    21961 atcaattgcc gcctcgtctg gtgccttcgc atcaggcaat ccacttgttg caaattgccc
    22021 gtgaggcatt aagtaacgcc ctcaaacatt cgcaagcgag tgaggtcgtg gtgacggtgg
    22081 cgcaaaacga taatcaggtc aaactgaccg tccaggataa cggctgcggc gtgcctgaaa
    22141 atgccatccg cagcaatcac tacggcatga taataatgcg cgaccgtgcg caaagtttac
    22201 gaggcgattg ccgcgtccgc cgtcgtgaat caggtggcac cgaagtggtg gtcaccttta
    22261 ttcccgaaaa aactttcaca gacgtccaag gagataccca tgagtaatca ggaaccggct
    22321 actatcctgc tgattgacga tcacccgatg ctgcgaactg gcgtaaaaca gcttatcagt
    22381 atggcaccag atatcaccgt ggttggcgaa gcgagtaatg gcgaacaggg tattgaactg
    22441 gcggaatctc ttgatcccga tctgatcctg ttagatctca atatgcccgg catgaacggt
    22501 ctggaaacgc tggataaact gcgcgaaaag tccctctcag ggcgcattgt ggtattcagc
    22561 gtctctaacc atgaagaaga tgtggtcacc gcactgaaac gcggcgcgga tggctatctg
    22621 ttaaaagata tggaaccgga agatctgctg aaagcattgc atcaggcagc tgctggcgaa
    22681 atggtattaa gcgaagcatt aacgcctgtt ctggccgcca gcttgcgcgc taaccgtgcc
    22741 acgactgagc gcgatgttaa ccagttaacc ccacgcgagc gcgatattct caagctgatt
    22801 gcccagggtt tgccgaacaa gatgattgcc cgccgcctgg acatcaccga aagcacagta
    22861 aaagtgcacg tcaagcacat gctgaagaaa atgaagctca agtctcgcgt ggaagcagcg
    22921 gtatgggtgc atcaggagcg cattttctga ttacggttcc cagcgcagtt cgtcgtttga
    22981 caatgcgtcg aacggttcga caagcgttag cgtgatctca ttggaggaga cacgctgccc
    23041 ctggttatct tccaccacca ccgacaatcg ccagtgattg ctcgcccctt ccccgttctg
    23101 ccagtcaggc atgatcagcg tccagccctc cgcgctgttg gcttgtgcgc ccggcgtcaa
    23161 acttaatatc tgcgtatcgc cctgccaaat cagttgccga ataccgtaac gactgcggat
    23221 ttgtaatttc agcggcactg tttcgccagg ttttagatcc cacggcggtg tcgcaagaaa
    23281 caccgttaac gtttttcgct gtcggtactc aagagtcggt agattatttc gctgcggatt
    23341 atcatagcga ctaccacgta acgactgact ttcggcaacc tcgcccgcag aaagttgttt
    23401 tttgagcggt acaccaaagc ggtaattaag attcagcccg aggttatttt gattctcgcc
    23461 actttcaccc tgtttatgct gggccgtcac agtgactaat ggcacagggg tgtaatttaa
    23521 tcccagactc aacgcgacgg gattgtgata acccgtacca gagttaaaca aatcaacacg
    23581 atcaccaaaa tactgttcta cgctgacact ggtattgagg tgttgataga acggcatgcg
    23641 catccgggct gtcaggtcgt acccgcgcgc catccgttgt tcctgcgtgg ctgtctgttc
    23701 atgccatgca gcaaacggct gataaaagtt tgccgatagt cgcaaatatt cgccccacgc
    23761 ttcggcacca aagcccgctc gctgaagatt ttcatccagc aagttgtcat aaaaagtgtt
    23821 ataacccacc agccagttgc cgcgcgccca gcgttgccca acgcccacat tgctcaccag
    23881 cccatcatcc tgctgagtaa gaccaagctg gctccaggtg agataacgat cattatcttg
    23941 taacggcaca aaccagcttc cacgactgcc ggtaaaatga ccttcgttat ccactttgac
    24001 gtcaacactg gcatttcccc acggtgatag ccacgactct acgtgctgat taacctgttg
    24061 actaagcgcg tcgcggactt ttcccaatgc gaaagctttt gcctgctcgc cagtatccag
    24121 cccgttatca ttcatactgg tttcgccgaa atctttcaca atttcagcaa agtgtttttc
    24181 cccatcatga ttttcaggcg ccatgcctaa atccggcaga ccatcattgt tattatcaaa
    24241 gggatttgcc gctttttgct cgaaggtaga ttgtgcgtta gctgtaccgc ctgccgccag
    24301 caagagtaaa taaaacggaa taatgcgagg aacgaaacgg ctcaaaacgt cggtaacttc
    24361 cggtaaaaaa tgaaaaaaca tagccttaaa gaataacgat tactggcaac ttttgtcgta
    24421 ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga cgctgcagaa
    24481 gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata atgcaaaaaa
    24541 tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac agcttgcggc
    24601 tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc ctgatgtctg
    24661 atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac atacagcaaa
    24721 tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg
    24781 ggcgcgggat tagtacactt cctctgattg atggggtgga aatcggtact cttgtggaac
    24841 tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg agggggcgtc
    24901 tgacgcccca cgatgaggga agtttactca ggcgaatccc ggccgcaaca caccatccgg
    24961 ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc catcgtcctg
    25021 cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt tggttcccag
    25081 cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt cagattcata
    25141 ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc catcatcaag
    25201 atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct tccacaacag
    25261 ggttagttcc tgctccagcg tctcttctgg cagtcgatag gcgacgcctg tggtacgtcc
    25321 gccctctttt agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag
    25381 gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcacgact cggtaaactc
    25441 cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc tctcatcagg
    25501 tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc ataagagtga
    25561 ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat cacgcgttat
    25621 caccttctta cacctccgcc actgcatgct tccctgcgac aactttttgg tgtcttccgg
    25681 acatttttac tgctcatttt gaaaccacta acgggacaat atgcaatccg cggaccactt
    25741 gcaaggcaac accgcacgaa tagctttaac taagctaccg gttttacgat tttttatgcc
    25801 atttatcatc atcccctttg gcatattcat gcttcacagc agcccaggcc actttatgcg
    25861 cggtttcttc gcgactggcg tcatcacgcc gatcttcttt atctttatat tgatcccatg
    25921 cgctgttgaa cgcttcttta tagatatcct gggcatgaga cggtagaacg tgctttacgc
    25981 tttccggcag gtcgcttttc gttttatacg gcatcgttga ctccttttac aaagttgaac
    26041 attaagtgtg gtcaacaata tctactcttg caaaaacata agaattttca tgtcagactg
    26101 ttcttaagaa acgctaaaac aaacattgcc taaacgtaat acttattttt atgccccaat
    26161 gagtaaaaaa tcgacaacgg ggtaaaaatt cgtaaaatgt taccgatatc cagacacttt
    26221 ctgttatttt tcctacctat tttttacatt ttcgataaat tcatctgcaa agggataacc
    26281 ataatgtcaa atgctcaaga ggcggtaaaa acccgccaca aggagacttc gcttattttc
    26341 ccggttctgg cgctggtagt gctgttcctg tggggaagca gccagacact accagtggtc
    26401 attgccatca atcttcttgc gcttattggt attttaagta gcgcctttag tgttgtccgt
    26461 catgcggacg tattagccca tcgcctggga gaaccttacg gttcgcttat tcttagcctt
    26521 tcagtggtta ttcttgaagt tagtttgatt tcagctttaa tggcaaccgg cgacgccgcg
    26581 ccaacgctaa tgcgtgatac gctctattcg atcattatga ttgttaccgg tgggctggtc
    26641 ggcttttcat tattgttggg cggtcgtaag tttgccaccc aatatatgaa tctgtttggt
    26701 attaagcagt atttaattgc gctgttcccc ctggcgataa tcgtactggt atttccaatg
    26761 gctctgcctg cggcgaattt ttcaaccggt caggcgttac tggtagcatt aatttctgcg
    26821 gcaatgtatg gtgtattttt gctgatccag accaaaacgc atcaaagttt gtttgtctac
    26881 gagcacgaag atgacagtga tgacgacgac ccgcaccacg gtaaaccgtc tgcccatagc
    26941 agcctgtggc atgctatctg gttgattatc cacctgattg ccgttattgc agtcaccaaa
    27001 atgaacgcca gcccgctgga gactttgctc gacagcatga atgcccctgt cgcctttact
    27061 ggcttcctgg tggcactgtt gattctgtcg ccggaaggtt taggtgcatt aaaagcagtg
    27121 ttgaacaacc aggtccagcg cgcgatgaat ctgttctttg gttcagtgtt agcaacaatt
    27181 tcgcttaccg tacctgtcgt cacgctaatt gcctttatga cgggtaacga attgcagttt
    27241 gcacttggtg cgccagaaat ggtggtgatg gtggcctctt tagtgctgtg ccatatctcc
    27301 ttctccaccg gacgtactaa cgtgctcaat ggcgcagcgc atctggcact gtttgccgcc
    27361 tatttgatga cgatatttgc ctgacagtta tatctcctcc gatgacggaa accgggggag
    27421 ataatataaa ttcctgctta ttgtatttct tcatactacc tggacaggtc gttaatcaga
    27481 attgcgagcc tgtaccacaa cctgcacgaa accactgcaa cagctgttga aaagccggaa
    27541 tgtactcttt attgcaaaca tttcagggat gaagtgaaga tgaattgaga cgtagtatcc
    27601 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggttttatc cctggtggca
    27661 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagggcta
    27721 atcttgaaac cagacacttc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct
    27781 atatgacgct cgcgcagttt gccatgactt tctggcacga cctggcggca ccgatcctgg
    27841 cgggaattat taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggtgaaaacg
    27901 ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg
    27961 cggccatttt tgtctttatc acttccacaa gtaaatattg gcaacccgag ctggaagcat
    28021 catccagagc actcatgaaa cgactgtaac agctgttgaa aaaccggaat gcactcttca
    28081 ttgcaaacat ttcagggatg aagtgaagat gaattgagtc gtagtatcca caccaagccg
    28141 gcgttatccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg
    28201 aaagagaaaa cccccgcacg ttgagaggta aaacctgaca acagaacggg ggctaatctt
    28261 gaaccatcaa caattcaaga atagccgcaa aatgttgtca ttacaacaag gcggctatat
    28321 gacgctcgcg cagtttgcca tgattttctg gcacgacctg gcagcaccga tcctggcggg
    28381 aattattacc gcagcgattg tcggctggtg gcgtaaccgg aagtaaggcg aaaacgggcg
    28441 tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg cagggtaaca gcctgcggcc
    28501 ttttttgtat ccggcaccca tgaaaaaagt cttaacgcag aacgctaaga ctttattttt
    28561 caagcaaaaa agattacttg ctggtatcca gttcttcgaa acctttcacc agatcatcaa
    28621 tcgctttcat ctgcctgagg aacggttcca gtttagccag cggcagcgcg gatggaccat
    28681 cacatttcgc atgttccgga tccggatgcg cttcaataaa cagccccgcc agacctaccg
    28741 ccataccggc tcgtgccagc tcagccacct gagcacgacg accgccggaa gctgcgccaa
    28801 aaggatcgcg gcattgcaat gcgtgggtca cgtcgaaaat caccggcgag ttaccagaca
    28861 ctttcttcat aatgctgaag cccagcatat cgacaaccag gttgtcatag ccgaagttag
    28921 caccacgatc gcaaagaatc actttttcgt tgccgccttc tttgaattta tcaacgatat
    28981 tacccatctg tcccgggctg acaaactgtg gtttcttgac gttaattacc gcaccggttt
    29041 tcgccatggc ttcaaccagg tcagtctggc gagcaagaaa cgccggcaac tgaatcacat
    29101 ccacgacatc agcaacgggc tgtgcctgac ttggttcgtg aacgtcggtg ataattttca
    29161 cgccaaaagt ctgcttcaac tcctggaaga ttttcatccc ttcttccagg cccggtccac
    29221 gataagagtg gatggaggag cggttggctt tgtcaaaaga ggctttgaac acgtaaggga
    29281 tacccagttt ctgggtcacg gttacgtagt gctcgcaaat gcgcatcgcc agatcgcgag
    29341 attccagcac gttcataccg ccaaacagta cgaacggcag gtcatttgct acgttgatgt
    29401 cgccaatgct aaccactttt tgtttcatag gatcgcctta accttgagta aaatgtcgat
    29461 taattaatgc agcacaatat gtttatgcgc gatgttattt atttgcgcac ggatcatttc
    29521 gctgatcggg tcttccggac actgttcaac gaaataactt aaatcgttca acgcaacgtg
    29581 ttcgcaatcc agttgcgcat aaatcaaccc gcgatcgcga atttcatagg gatcttccgg
    29641 gttgaattgt aataaagctt cgctggtgcg taacgccagc tccatctgat tttcttccat
    29701 caacgaggct ttgagtgtat ccagcaattt gcggattacc tcaatgttat cagcttcatc
    29761 aaggtcttca taaaacagtt ccgccgacgg gctgatattt ccctttaacc atacgtccag
    29821 catatgttcg cttaacgatt caccgttaaa aggattaatc agccaaattt cgccatccgg
    29881 acattcaatg cgcaatatca gctgcgtagg gaaaatcacc ggcagcagcg gcaaatcgag
    29941 acgattcgcg acccataata aaaccgcacc taatgatacc gcactgccct gtcgattctt
    30001 taacacctgg tccagccata atgcatcgga aagacgataa acgccgcgtg aggctttaaa
    30061 tccccagtcg ccgtaaaaca gcgcgatcag tttttccagc tgctcttcta aaggcagaag
    30121 ctggctgatt tcttccttcg ccagactaac gagacgctcc agttcgtcgt aaacatcttg
    30181 cgagggaaaa tcgcggcgga ttgcttcgca agccaggatc atgccttcgc acaatggcgc
    30241 tttattaaat tcgaaatcag ctaacgatct catgacttac cccagtaacg gtacttttgt
    30301 ggtggcgagt ttaatgatga tgtacagcac caccaacgtc agcgggaagg caataatgcg
    30361 cgcctgctga ctgtgcatac gacgataatc gagtgcaata aaacccaaaa cgatataaat
    30421 gataactcca aacagctttt cagtcagcca ctgtgcctgc ccggaaaatg gcaggatgtg
    30481 cgctttagcc atcaacgcaa tgccgcttaa caataacacc gtatcgacaa ccggcggcac
    30541 aatgcgcgtc cagcgagcaa atgcctgtgg atgcttctga taacgtagcc agaaacgtaa
    30601 ggttaatagc ccaacagaaa gcgcgatact aataagatga acactaagca gtgtagaaaa
    30661 acttgtcatt gataatagcg gccgagcgtt acgcgctcgt tatcaccata gtcacggcag
    30721 gtttcgacgt catgataccc cgcgaggata aatgcctgtc gcaccgcttc gccctgctgc
    30781 cagccatgtt ccagaagcag aaagccgccg gataccagcg cgttacgcga ctgttcgatg
    30841 atatgcacga tgtctgccat tccactgtct gccgcaacca gcgcagtgag cggctcaaag
    30901 cggacatcgc cttgttgaag atgagggtcc tgctcgtcaa tatacggcgg attgctgaca
    30961 atcatcgcaa actgctgccc ggctagcgcg ctaaaccagt cgctttgcag aatgtggata
    31021 tttttgatcg ccagatgctg ggcattacgt tgtgccaggg cgacagcatc gggcatacga
    31081 tctacagcgg taatttcgca gtccgggcgc tcgcgagcca gcgcaagcgc aatcgccccg
    31141 gtacccgtcc cgagatcgag aatacggcaa ggttgttcag gcaaccgcgc cagtgcctgc
    31201 tccaccagac actccgtatc cgggcgcgga attaaggtcg ctggcgaaac aaataacggc
    31261 aacgaccaga attctcgcac cccggttaaa tgagcaatgg gttcaccatc gcgacgacgt
    31321 gtcagtagcg catcaagttg ctgacattgt tcgtcagtca gctgcgtttc accaaaggcg
    31381 agaataaaag tacgccctct gccggtaaca tgctccagca ggatttcagc atcacgccgc
    31441 gggctttcgc tcgcctgaag ttggcttatt gcttcacgta accagtgttg atattccatt
    31501 attcctgctc ggacaacgcc gccagttggt cggcctgatg ttcctggata atcggttcaa
    31561 tcagcatatc cagcttacct tccatcactt catccaggcg gtagagcgtc aggttgatgc
    31621 ggtgatcggt aacgcgcccc tgcgggaagt tgtaagtacg gttacggtcg ctgcgatcgc
    31681 cactccccag caggttacga cgggtagacg cttcggcctg ttgccgtttt gccatttcag
    31741 cagcgtggat gcgagcaccg agtacagaaa gtgctttagc tttgttttta tgttgtgaac
    31801 gttcgtcctg acattcaaca acaatcccgg tcggcaagtg agtaatacga attgccgaat
    31861 cggtggtgtt aacgtgctga ccacccgccc ctgacgagcg gaaagtatca atgcgtaaat
    31921 ctgctgggtt gatgtccggc agttctgcgt caggcagttc tggcattacc gcaacggtac
    31981 aagcagaagt atgaatacga ccctgcgatt ccgtagcagg aacacgctgc acgcgatgac
    32041 cgccagattc aaatttcaga cgaccataca caccatcacc gctaattttg gcgatgatct
    32101 ctttataacc accatgttca ccctcgctgg cgctcatgat ttctacccgc cagcggcggg
    32161 cttcggcgta acggctgtac atacggaaca gatcgcctgc gaacagcgct gcttcgtcgc
    32221 cgccggttcc ggctcggact tcgaggaagg cgttacgttc gtcatcagga tcttttggca
    32281 gtaacagaac ctgtaattgc tgttccagtt gctcgctttt ttctttagct tcgcgcagtt
    32341 catcctgcgc catctcacgc atttcaggat catcgagcat catctgtgcg gtttcgatat
    32401 cttcctgaac ctgttgccag tcggtaaaac agcgcgaaac atcacttaac tgcgcatatt
    32461 cgcgtgataa tgcgcgaaaa cgttcctggt cggcgatagt ttgcgcgtca cccagcaacg
    32521 cctgaacttc ttcatggcgt tcatgcaggg cttccagttt ggcaacgata gaaggcttca
    32581 taggcgtaaa tgcaccctgt aaaaaaagaa aatgatgtac tgctactcca gcccgaggct
    32641 gtcgcgcaga atattcaggc gttcgttatc cccgtcacgg gcggcctgtt gaagtgattt
    32701 cgttggcgca tggatcaagc ggttagtcag tttccatgcc agatcctgca taatggcttg
    32761 cgcgtcgccg ccctgctcaa gggccgctaa cgctttggcg gttaactcat cgcgaacttg
    32821 ctctgcctgg ctgcgatact cgcgaatggt ttcgctggcg ctttgtgctc gcagccacgc
    32881 cataaattcg ctggtttcct gagcgacaat agtttccgcc tcaaccgctg cggctttacg
    32941 ctgcgccagg ttgtgcgaaa tgatgctttg cagatcatca acgctataaa gataagcatt
    33001 cgccagtttg ccaacttccg gctcaacatc gcgcggaacg gcaatatcca ccaacagcat
    33061 tggttggttg cgacggcttt ttaatgcgcg ctccaccatg cctttcccga taatcggtaa
    33121 cgggctggcg gtggaactga tgatgatatc ggcttcgcgc agacgttcgt cgatatcact
    33181 cagggcaatc acttccgcgc cgacttcatc tgccagaatt tgggcacgtt cgcgagtgcg
    33241 gttggcgata atcatcttct gtactttatg ttcgcgcaga tgacgcgcta ccagctcgat
    33301 ggtttcgccc gcgcctacca gcaacactgt gaccgtagag agcgattcaa agatctgccg
    33361 cgccagcgta caagccgcaa aagcgacaga cacagcgctg gcaccgatat ctgtttcagt
    33421 gcgtacgcgt ttcgctacag agaaagattt ctggaacatg cgttccagtt cgctggcctt
    33481 catatgacct ttttgcgaat cggcaaacgc ttttttaacc tgaccgagga tctgcggctc
    33541 cccaagaacc agtgaatcca ggccgctggc aacacgcatt aaatggctaa ccgcgtcgtt
    33601 atcctgatgc cagtagaggc ttttacgcag atcttcttca ttaagattgt gataatcgca
    33661 aagccagcgg attaacgcct cttgcaagtt gtcctgctct tcgacgctaa gataaagctc
    33721 agtgcggtta cacgtcgaca gcaccacccc gccctgcacc atcggctgtg cgagcagact
    33781 gtccagcgcc tgatcaagtt tgtccggcga aaacgataca cgttctcgca gcgatacagg
    33841 tgccgtttta tggttaatac cgagcgctaa aagggtcatg tctgcgggaa ataataccaa
    33901 cgttgataag gttagtctcg gcgcatcata ccgtatgcgc gagatcaata aaagggagcg
    33961 cccccttttg gagtaattgc ccgaacccgt tagatttcta caattaagac aacttgaaca
    34021 tagacgatga ctaatggtga cgctagcatt aagggttata actgcaacgt atctcaagga
    34081 cttgtcatca ctatgcccct gcccgatttt cgtcttatcc gcctgctacc gctggctgct
    34141 cttgtgctca ctgcctgttc cgttaccacg cccaaaggtc ctggcaaaag cccggattcg
    34201 ccacaatggc gtcagcatca gcaagacgtg cgcaatctta atcagtatca gactcgcggc
    34261 gcgttcgctt atatttctga ccaacaaaaa gtgtacgccc gctttttctg gcagcaaacc
    34321 ggccaggatc gctaccgtct gctgctcact aacccattgg gcagcacgga actggagctg
    34381 aatgctcaac cgggtaacgt gcagttagtc gacaataaag gtcagcgtta taccgccgat
    34441 gacgccgaag agatgattgg caaattgact ggaatgccaa ttccgctcaa cagcttgcgc
    34501 cagtggattt taggtttacc gggtgatgca accgactaca aactggacga ccagtaccgc
    34561 ctgagcgaaa ttacctacag ccagaatggc aaaaactgga aggttgttta tggtggttat
    34621 gacaccaaaa cgcaacctgc gatgccagcc aatatggaac tcaccgacgg tggtcaacgc
    34681 atcaagttaa aaatggataa ctggatagtg aaataatgcg gacacagtgg ccctctccgg
    34741 caaaacttaa tctgttttta tacattaccg gtcagcgtgc ggatggttac cacacgctgc
    34801 aaacgctgtt tcagtttctt gattacggcg acaccatcag cattgagctt cgtgacgatg
    34861 gggatatccg tctgttaacg cccgttgaag gcgtggaaca tgaagataac ctgatcgttc
    34921 gcgcagcgcg gttgttgatg aaaactgcgg cagacagcgg gcgtcttccg acgggaagcg
    34981 gtgcgaatat cagcattgac aagcgtttgc cgatgggcgg cggtctcggc ggtggttcat
    35041 ccaatgccgc gacggtcctg gtggcattaa atcatctctg gcaatgcggg ctaagcatgg
    35101 atgagctggc ggaaatgggg ctgacgctgg gcgcagatgt tcctgtcttt gttcgggggc
    35161 atgccgcgtt tgccgaaggc gttggtgaaa tactaacccc ggtggatccg ccagagaagt
    35221 ggtatctggt ggcgcaccct ggtgtaagta ttccgactcc ggtgattttt aaagatcctg
    35281 aactcccgcg caatacgcca aaaaggtcaa tagaaacgtt gctaaaatgt gaattcagca
    35341 atgattgcga ggttatcgca agaaaacgtt ttcgcgaggt tgatgcggtg ctttcctggc
    35401 tgttagaata cgccccgtcg cgcctgactg ggacaggggc ctgtgtcttt gctgaatttg
    35461 atacagagtc tgaagcccgc caggtgctag agcaagcccc ggaatggctc aatggctttg
    35521 tggcgaaagg cgctaatctt tccccattgc acagagccat gctttaagcc gggcaagctg
    35581 agtttcggtg acaacgtcac cttgttccag acgttgcatc gcgctcttta atacaccgcc
    35641 tggaaaggat catgcctggc ccgcacagtt ttcggcagat tctttccacc aatggacgca
    35701 tgcctgaggt tcttctcgtg cctgatatga agctttttgc tggtaacgcc accccggaac
    35761 tagcacaacg tattgccaac cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct
    35821 ttagcgatgg cgaagtcagc gtacaaatta atgaaaatgt acgcggtggt gatattttca
    35881 tcatccagtc cacttgtgcc cctactaacg acaacctgat ggaattagtc gttatggttg
    35941 atgccctgcg tcgtgcttcc gcaggtcgta tcaccgctgt tatcccctac tttggctatg
    36001 cgcgccagga ccgtcgcgtc cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag
    36061 acttcctctc cagcgtcggt gttgaccgtg tgctgacagt ggatctgcac gctgaacaga
    36121 ttcagggttt cttcgacgtt ccggttgata acgtatttgg tagcccgatc ctgctggaag
    36181 acatgctgca gctgaatctg gataacccaa ttgtggtttc tccggacatc ggcggcgttg
    36241 tgcgtgcccg cgctatcgct aagctgctga acgataccga tatggcaatc atcgacaaac
    36301 gtcgtccgcg tgcgaacgtt tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg
    36361 actgcgtact ggtcgatgat atgatcgaca ctggcggtac gctgtgtaaa gctgctgaag
    36421 ctctgaaaga acgtggtgct aaacgtgtat ttgcgtacgc gactcacccg atcttctctg
    36481 gcaacgcggc gaacaacctg cgtaactctg taattgatga agtcgttgtc tgcgatacca
    36541 ttccgctgag cgatgaaatc aaatcactgc cgaacgtgcg tactctgacc ctgtcaggta
    36601 tgctggccga agcgattcgt cgtatcagca acgaagaatc gatctctgcc atgttcgaac
    36661 actaatcgaa cccggctcaa agacccgctg cggcgggttt ttttgtctgt aatatccatt
    36721 tgtatgacct atgcctcctt cacctgccat ttagttgaca gatgatgcgc tcacggatga
    36781 aacattattg tgaacaaaat attttcctca catgtgatgc ctttccgcgc tctgatcgac
    36841 gcttgctgga aagaaaaata tactgccgca cggtttaccc gtgacctgat tgccgggata
    36901 accgtcggga ttattgctat cccgctggcg atggcgttgg ctattggtag tggtgtggca
    36961 ccccagtacg gtttatatac cgcagctgtt gcggggattg tcattgctct gacgggtggg
    37021 tcacgcttta gcgtttccgg tccgactgcg gcatttgtgg taattctcta tcccgtttcg
    37081 caacagtttg ggctggcagg actgctggtt gcgaccttgc tgtcggggat ctttttgatt
    37141 ctgatgggtc tggcacgctt tggtcgcctg attgagtata ttccggtttc cgtcacctta
    37201 ggtttcacct cgggtatcgg gatcaccatc ggtaccatgc agattaaaga ttttctcggt
    37261 ctgcaaatgg cccatgtccc ggaacattat ctacaaaaag tcggcgcatt atttatggcg
    37321 ctgccgacca ttaatgtggg tgatgctgcc attggcattg tgacgctagg tattcttgtt
    37381 ttctggccgc gtctgggcat tcgtttaccc ggtcaccttc cggccttgct ggctggttgc
    37441 gcggtgatgg ggattgttaa cctgctcggc ggacatgttg ctaccatcgg ttcgcaattc
    37501 cactacgtcc tggccgatgg ttctcagggt aacggtattc cgcaactgct accgcaactg
    37561 gtgctgccgt gggatctgcc taattcagaa ttcacgctaa cctgggattc tattcgcaca
    37621 ctgctgcctg cggcattctc aatggcaatg ctcggcgcaa tcgaatctct gctctgcgcc
    37681 gtggtgctgg atggtatgac cgggacgaag cacaaggcga atagcgaact ggttggacag
    37741 gggctgggga atatcatcgc tccgttcttt ggtggtatta ccgctaccgc tgccatcgcg
    37801 cgttctgccg ctaacgtccg tgccggggca acttccccta tctcggcggt gatccactct
    37861 attctggtta ttcttgccct gctggtactg gcaccgctgc tctcctggct gccgctttcc
    37921 gctatggcag ccctgctgtt gatggtggcg tggaacatga gtgaagcgca taaagtggtc
    37981 gacttgctgc gtcatgcacc gaaagatgac atcattgtca tgctgctgtg catgtcgctg
    38041 accgtgctgt ttgatatggt tattgccatc agcgtgggga tcgtgctggc atcgctgctg
    38101 tttatgcgtc gtatcgcacg tatgactcgc ctggcaccgg tagtcgtaga tgttccagac
    38161 gatgtcctgg ttctgcgcgt tattggcccg ctgttttttg ctgctgctga aggcttattc
    38221 acggacctgg agtcacgtct tgaaggcaaa cggattgtga ttctgaagtg ggatgccgtt
    38281 ccggtacttg atgctggtgg tcttgatgcg ttccagcgtt ttgtgaagcg tctgcccgaa
    38341 ggatgtgaac tgcgcgtgtg caacgtggaa ttccagccac tgcgcactat ggctcgcgca
    38401 ggcattcaac cgatcccggg acgcctcgcg ttcttcccga atcgtcgcgc ggcgatggcg
    38461 gatttataag tacgagattg accagtcagc gcaaactgac tggtcagcaa actgcattat
    38521 ttgttagcta tgacggcgat tatcgctacg gcggcaacgt ttgtcatagt ggcgaaccca
    38581 ccagtaacgg tcgcacacct gttcatgccc gccaacacgg gcacccgcca gccagagaat
    38641 ggcaccgacg aaaatactta gcactgcacc atgtgcgaaa tactggggca tattaaactg
    38701 tggtaactgg ttgaggattg aataccccac gccgaccacc attaccacca gacccaaccc
    38761 catgagcacg ttaccgagta acgaagcgtt tttgcgtttc atatgtcacc tccggaactt
    38821 tctgggttgt aacagggaat acccctcttc cttatgtgta aagtatagac aacaccctgt
    38881 gggttatgtg cgggcgtgat cacaattaca acccttattt caacaaaact ttacaaataa
    38941 acgcctgaac accttgactt tctaatagtc cactcaggca attattacgt tatctgcatt
    39001 tgatacgcag ttttttgtca agcggggccg caaccagtaa actacgcgcc agttatgtac
    39061 acactcagga caaaaaaacg tgacgattaa attgattgtc ggcctggcga accccggtgc
    39121 tgaatacgcc gcaacgcgac ataatgctgg tgcctggttc gttgacttac tggcagagcg
    39181 tttgcgcgct ccgctgcgcg aagaggctaa attctttggt tatacttcgc gagtcaccct
    39241 tggaggcgaa gatgtccgcc tgttagtccc gactacattt atgaatctca gcggcaaagc
    39301 cgttgcggcg atggccagtt ttttccgcat taatccggac gaaattctgg tggcccacga
    39361 cgaactggat ctgcctcctg gcgtcgccaa atttaagttg ggcggtggcc atggtggtca
    39421 caatggactg aaagacatca tcagtaaatt gggtaataac cctaactttc accgtttacg
    39481 catcggaatc ggtcatccgg gcgataaaaa caaagttgtt ggttttgtgt taggcaaacc
    39541 gcctgttagt gaacagaagt taattgatga agccatcgac gaagcggcgc gttgtactga
    39601 aatgtggttt acagatggct tgaccaaagc aacgaaccga ttgcacgcct ttaaagcgca
    39661 ataagtcgtt gtctgcggca tttttgccga gtgccgtgta taataggcaa agttatttcc
    39721 atttctgcaa tctgttagca ataacaggtt gattattaag atattaaggt gatttaaatc
    39781 atgggattca aatgcggtat cgtcggtttg cccaacgtcg ggaaatctac cctgttcaac
    39841 gcgctgacca aagccggtat tgaagcggcc aactttccat tctgcaccat tgagccgaac
    39901 acaggcgtcg taccaatgcc tgatcctcgc ctggatcaac tggctgaaat cgtaaaaccg
    39961 caacgtacgc ttcccacgac catggaattt gtcgatatcg ccggtctggt aaaaggcgca
    40021 tcgaaaggcg aaggtctggg taaccagttc ctgaccaaca tccgtgaaac cgaagcgatc
    40081 ggtcacgttg ttcgctgctt tgaaaatgac aacatcattc acgtttccgg caaagttaac
    40141 ccggctgacg atattgaagt tatcaacacc gaactggcgc tggcagacct cgacacctgc
    40201 gaacgtgcga ttcatcgcgt acagaagaaa gccaaaggtg gcgataaaga cgcgaaagct
    40261 gagctggcgg tcctggaaaa atgcctgccc cagctggaaa acgcaggtat gctgcgcgcg
    40321 ctggatttaa gcgctgaaga gaaagcggct attcgttacc tgagcttcct gacgctgaaa
    40381 ccaacaatgt acatcgccaa cgtcaacgaa gacggttttg aaaacaaccc atatcttgac
    40441 caggtgcgtg aaatcgcggc gaaagaaggt tctgttgtgg ttccggtttg tgctgctgtt
    40501 gaagcagaca ttgccgaact ggacgacgaa gaacgtgacg agtttatgca ggagcttggg
    40561 ctggaagagc cgggtctgaa ccgtgtgatc cgtgccggtt ataagctgct gaacctgcaa
    40621 acttacttca ccgctggggt gaaagaagtg cgtgcatgga ccattccggt tggagcaacc
    40681 gcgccgcagg cagcgggcaa aatccatact gattttgaaa aaggctttat ccgtgcacaa
    40741 accatctcgt ttgaagattt catcacttac aaaggtgaac aaggcgcgaa agaagcaggc
    40801 aaaatgcgtg cagaaggtaa agattacatc gtgaaagatg gcgatgtgat gaacttcctt
    40861 ttcaacgtct aattaattta ttgtctcatg aggtttcgct aaatctcatg aagagcgcaa
    40921 aagtatataa aatccacgca attgcgtgga tttttcattt caccgagtcc taatcattct
    40981 tgtaacatcc tacacttttg tgcttattgc ctacagtgaa ccacgagaat aattaaagac
    41041 atcagtgttc agatgacgga tatctcatta gcagtgatat aacgtcagtt tttgttccac
    41101 tacacatcag cccccttcac agccgatcaa tattgaccac gctccaatat gctggcgaga
    41161 aaaggactta cctgaagggt aatatcccgc agataaagtc cccgaataac gtgattacgt
    41221 gcgatcctgg caagatgaaa atgataacca gtagcacaat aacaacgata gaccagaaaa
    41281 cccattgacc aaaaatgaat atcgatgttt tttgcttttt cttattagta atgcaaaaat
    41341 ggcgcatatt gtttttgtta tgcagataca tcaag
//
LOCUS       DAZFTN010000031        41361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFTN010000031 DAZFTN010000000
VERSION     DAZFTN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(313..576)
                     /gene="cedA"
                     /locus_tag="ACHKV2_001810"
     CDS             complement(313..576)
                     /gene="cedA"
                     /locus_tag="ACHKV2_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO6939704.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            759..3020
                     /gene="katE"
                     /locus_tag="ACHKV2_001811"
     CDS             759..3020
                     /gene="katE"
                     /locus_tag="ACHKV2_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO6939705.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3067..3825)
                     /gene="chbG"
                     /locus_tag="ACHKV2_001812"
     CDS             complement(3067..3825)
                     /gene="chbG"
                     /locus_tag="ACHKV2_001812"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO6939706.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSYGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3838..5190)
                     /gene="celF"
                     /locus_tag="ACHKV2_001813"
                     /gene_synonym="chbF"
     CDS             complement(3838..5190)
                     /gene="celF"
                     /locus_tag="ACHKV2_001813"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6939707.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEMINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5296..6138)
                     /gene="chbR"
                     /locus_tag="ACHKV2_001814"
     CDS             complement(5296..6138)
                     /gene="chbR"
                     /locus_tag="ACHKV2_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO6939708.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6146..6496)
                     /gene="chbA"
                     /locus_tag="ACHKV2_001815"
     CDS             complement(6146..6496)
                     /gene="chbA"
                     /locus_tag="ACHKV2_001815"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO6939709.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6547..7905)
                     /gene="chbC"
                     /locus_tag="ACHKV2_001816"
     CDS             complement(6547..7905)
                     /gene="chbC"
                     /locus_tag="ACHKV2_001816"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO6939710.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7990..8310)
                     /gene="chbB"
                     /locus_tag="ACHKV2_001817"
     CDS             complement(7990..8310)
                     /gene="chbB"
                     /locus_tag="ACHKV2_001817"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO6939711.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8610..8948)
                     /gene="osmE"
                     /locus_tag="ACHKV2_001818"
     CDS             complement(8610..8948)
                     /gene="osmE"
                     /locus_tag="ACHKV2_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO6939712.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9150..9977
                     /gene="nadE"
                     /locus_tag="ACHKV2_001819"
     CDS             9150..9977
                     /gene="nadE"
                     /locus_tag="ACHKV2_001819"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO6939713.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10207..11094
                     /gene="cho"
                     /locus_tag="ACHKV2_001820"
     CDS             10207..11094
                     /gene="cho"
                     /locus_tag="ACHKV2_001820"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO6939714.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11054..11629)
                     /gene="ves"
                     /locus_tag="ACHKV2_001821"
     CDS             complement(11054..11629)
                     /gene="ves"
                     /locus_tag="ACHKV2_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO6939715.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11832..12317)
                     /gene="spy"
                     /locus_tag="ACHKV2_001822"
     CDS             complement(11832..12317)
                     /gene="spy"
                     /locus_tag="ACHKV2_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO6939716.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12647..13615)
                     /gene="astE"
                     /locus_tag="ACHKV2_001823"
     CDS             complement(12647..13615)
                     /gene="astE"
                     /locus_tag="ACHKV2_001823"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO6939717.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13608..14951)
                     /gene="astB"
                     /locus_tag="ACHKV2_001824"
     CDS             complement(13608..14951)
                     /gene="astB"
                     /locus_tag="ACHKV2_001824"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO6939718.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKNLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14948..16426)
                     /gene="astD"
                     /locus_tag="ACHKV2_001825"
     CDS             complement(14948..16426)
                     /gene="astD"
                     /locus_tag="ACHKV2_001825"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO6939719.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16423..17457)
                     /gene="astA"
                     /locus_tag="ACHKV2_001826"
     CDS             complement(16423..17457)
                     /gene="astA"
                     /locus_tag="ACHKV2_001826"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO6939720.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17454..18674)
                     /gene="astC"
                     /locus_tag="ACHKV2_001827"
     CDS             complement(17454..18674)
                     /gene="astC"
                     /locus_tag="ACHKV2_001827"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO6939721.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            19120..19926
                     /gene="xthA"
                     /locus_tag="ACHKV2_001828"
     CDS             19120..19926
                     /gene="xthA"
                     /locus_tag="ACHKV2_001828"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO6939722.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20093..20803
                     /gene="ydjX"
                     /locus_tag="ACHKV2_001829"
     CDS             20093..20803
                     /gene="ydjX"
                     /locus_tag="ACHKV2_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6939723.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20808..21485
                     /gene="ydjY"
                     /locus_tag="ACHKV2_001830"
     CDS             20808..21485
                     /gene="ydjY"
                     /locus_tag="ACHKV2_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO6939724.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            21500..22207
                     /gene="ydjZ"
                     /locus_tag="ACHKV2_001831"
     CDS             21500..22207
                     /gene="ydjZ"
                     /locus_tag="ACHKV2_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO6939725.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22207..22755
                     /gene="ynjA"
                     /locus_tag="ACHKV2_001832"
     CDS             22207..22755
                     /gene="ynjA"
                     /locus_tag="ACHKV2_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6939726.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22765..23931
                     /gene="ynjB"
                     /locus_tag="ACHKV2_001833"
     CDS             22765..23931
                     /gene="ynjB"
                     /locus_tag="ACHKV2_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6939727.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23904..25439
                     /gene="ynjC"
                     /locus_tag="ACHKV2_001834"
     CDS             23904..25439
                     /gene="ynjC"
                     /locus_tag="ACHKV2_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO6939728.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25439..26092
                     /gene="ynjD"
                     /locus_tag="ACHKV2_001835"
     CDS             25439..26092
                     /gene="ynjD"
                     /locus_tag="ACHKV2_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6939729.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26159..27466
                     /gene="ynjE"
                     /locus_tag="ACHKV2_001836"
     CDS             26159..27466
                     /gene="ynjE"
                     /locus_tag="ACHKV2_001836"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO6939730.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27475..28095)
                     /gene="ynjF"
                     /locus_tag="ACHKV2_001837"
     CDS             complement(27475..28095)
                     /gene="ynjF"
                     /locus_tag="ACHKV2_001837"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6939731.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28182..28589
                     /gene="nudG"
                     /locus_tag="ACHKV2_001838"
     CDS             28182..28589
                     /gene="nudG"
                     /locus_tag="ACHKV2_001838"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO6939732.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28555..28827)
                     /gene="ynjH"
                     /locus_tag="ACHKV2_001839"
     CDS             complement(28555..28827)
                     /gene="ynjH"
                     /locus_tag="ACHKV2_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO6939733.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29063..30406
                     /gene="gdhA"
                     /locus_tag="ACHKV2_001840"
     CDS             29063..30406
                     /gene="gdhA"
                     /locus_tag="ACHKV2_001840"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO6939734.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30523..31563)
                     /gene="ynjI"
                     /locus_tag="ACHKV2_001841"
     CDS             complement(30523..31563)
                     /gene="ynjI"
                     /locus_tag="ACHKV2_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6939735.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31691..33652)
                     /gene="topB"
                     /locus_tag="ACHKV2_001842"
     CDS             complement(31691..33652)
                     /gene="topB"
                     /locus_tag="ACHKV2_001842"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6939736.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33657..34700)
                     /gene="selD"
                     /locus_tag="ACHKV2_001843"
     CDS             complement(33657..34700)
                     /gene="selD"
                     /locus_tag="ACHKV2_001843"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO6939737.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34817..35368)
                     /gene="ydjA"
                     /locus_tag="ACHKV2_001844"
     CDS             complement(34817..35368)
                     /gene="ydjA"
                     /locus_tag="ACHKV2_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO6939738.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35529..37385
                     /gene="sppA"
                     /locus_tag="ACHKV2_001845"
     CDS             35529..37385
                     /gene="sppA"
                     /locus_tag="ACHKV2_001845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO6939739.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37501..38211
                     /locus_tag="ACHKV2_001846"
     CDS             37501..38211
                     /locus_tag="ACHKV2_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO6939740.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            38343..39359
                     /gene="ansA"
                     /locus_tag="ACHKV2_001847"
     CDS             38343..39359
                     /gene="ansA"
                     /locus_tag="ACHKV2_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO6939741.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            39370..40011
                     /gene="pncA"
                     /locus_tag="ACHKV2_001848"
     CDS             39370..40011
                     /gene="pncA"
                     /locus_tag="ACHKV2_001848"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO6939742.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(40104..>41361)
                     /gene="ydjE"
                     /locus_tag="ACHKV2_001849"
     CDS             complement(40104..>41361)
                     /gene="ydjE"
                     /locus_tag="ACHKV2_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939743.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT        10282 a        10874 c        10164 g        10041 t
ORIGIN      
        1 ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat tattatctca
       61 tttaaatcta agtcatttac aatataagtt taagagcgac gccacaggat gaactatcaa
      121 aaatagctca tcatgattag caaaacttaa ccattttaaa ataaataaac aattaaagaa
      181 aaaagatcac ttatttatag caatagatcg tcaaaggcag ctttttgtta caggtggttt
      241 gaatgaatgt agcaatgaaa tagagaattt caggccatgt aactcccggc aaaaccggga
      301 ggtacgtaat ctttactcag tcacttcccc ttcctggcgg atctgatttg cccaacgctg
      361 ggcggattca ggcacagtaa acgccggtga gcgcagaaat gactctccca tcagtacaaa
      421 cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct ttatcgcatg
      481 ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt tttgctggcg
      541 gagcggtttc ttcattactg gcttcactaa acgcatatta aaaatcagaa aaactgtagt
      601 ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac cgtttccaga
      661 atagtctccg aagcggggtc tggctggtgg tctatagtta gagagttttt tgaccaaaac
      721 agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacaa aacgaaaaga
      781 acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg gggatggact
      841 cactggcacc tgaggacggc tctcatcgtc cagcggctga accaacaccg ccaggtgcac
      901 aacctaccgc ctcagggagc ctgaaagccc ctgatacgcg taacgaaaaa cttaattctc
      961 tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca
     1021 tcgccgacga tcaaaactca ctgcgtgccg gtagccgtgg tccaacgctg ctggaagatt
     1081 ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa cgtattgttc
     1141 atgcacgtgg atcagccgct cacggttatt tccagccata taaaagctta agcgatatca
     1201 ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt aggttctcta
     1261 ccgttcaggg tggtgctggc tctgccgata ccgtgcgtga tatccgtggc tttgccacca
     1321 agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca atcttcttta
     1381 tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa ccacactggg
     1441 caattccaca agggcaaagc gcccacgata ctttctggga ttatgtttct ctgcaacctg
     1501 aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc agttaccgca
     1561 ccatggaagg ctttggtatt cataccttcc gcctgattaa tgccgaaggg aaggcaacgt
     1621 ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg gatgaagcac
     1681 aaaaactaac cggacgtgac ccggacttcc accgccgcga gttgtgggaa gccattgaag
     1741 caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa gatgaattca
     1801 agttcgactt cgatcttctc gatccaacta aacttatccc ggaagaactg gtgcccgttc
     1861 agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc gaaaacgaac
     1921 aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac gatcccctgt
     1981 tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt gggccgaatt
     2041 tccatgagat tccgattaac cgcccaacct gcccttacca taatttccag cgtgacggca
     2101 tgcatcgtat ggggatcgac actaacccgg cgaattacga accgaactcg atcaacgata
     2161 actggccgcg cgaaacaccg ccgggaccga aacgcggcgg ttttgaatca tatcaggaac
     2221 gcgtggaagg aaataaagtt cgcgagcgca gtccatcttt tggcgaatat tattcccatc
     2281 cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc gatggtttca
     2341 gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg
     2401 cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg
     2461 acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag gatccatcct
     2521 taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg attttactta
     2581 atgatgaagt gagatcggca gaccttctgg ccattctcaa ggcgctgaag gccaaaggcg
     2641 ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt
     2701 tgcctatagc cgctaccttt gccggtgcgc cttcgctgac ggtcgatgcg gtcattgtcc
     2761 cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactac ctgatggaag
     2821 cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt aaagcaacaa
     2881 tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc tgacagcgcc gacggtagtt
     2941 ttatggatga actgttaacg ctgatggcag cacaccgcgt gtggtcacgc attcctaaga
     3001 ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt gaccttacca
     3061 ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa ttgcatattt
     3121 caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt aagcactctg
     3181 gcgaatcgta ttatcgacaa acgccggatg gcacatgact tccagcgagc gttccccgcg
     3241 atggctggaa tcatcaagca cttgcaggaa cagtgcctcg ctaatctctt caccataaaa
     3301 tgcgctactg aatccgtacg aactgcgcag attagccggt aaatcaccag cgttagatag
     3361 cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg caacaatagg
     3421 gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag gtttgcgtcc
     3481 aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt cttccagcgg
     3541 taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat cgcgggttaa
     3601 ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagttcatcc ctatggccag
     3661 actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat tcaccagcgc
     3721 cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt tctgaccttt
     3781 gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca gcctcggtta
     3841 atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt tctcgtgcgc
     3901 cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca acggactaag
     3961 gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc tggcagcaat
     4021 ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat gcgtaatgcg
     4081 cggatgtggc gtcgcgccat cgcgccccag cgtacaggtc atttctaccg cccagtctgc
     4141 cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttctg cttgcttatc
     4201 gttgtagata gcgttgatca tttcgcacgc tgcatcagag taataagccc caccgcgctg
     4261 ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa aaagttgttt
     4321 ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc ccatttcaat
     4381 agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca acagattcag
     4441 agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc cagatgcttt
     4501 taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc catttaccag
     4561 cacatcctta atgaacacca tatggttgag gccgaacaga tcgatagata aatcatcgct
     4621 gtctttcagc atcagaacat cgcgaataaa catcttcatg ccgatcggaa tattacacac
     4681 gccgataaag cgtttaaatc cggtatgacg ataaacggct tcagtgacca ttcccgccgg
     4741 gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt ttacgatgtc
     4801 aaaaatcacc ggaatggtac gcagaccttt aaacagaccg cccgcgccgt tggtttcctg
     4861 accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg gtaattggcc
     4921 aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc gatccagcgt
     4981 tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga gatcgaaaat
     5041 aatatccagt ttagctttgc caccttcgac atccaccagc cataattcgc tgaccggcaa
     5101 ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc tcccgccacc
     5161 aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag aatactgata
     5221 tctggcatat ctgccccccc cggacataaa taatccagca acaggacaga tatgtgaatt
     5281 gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatagc tcttaggcgt
     5341 aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat aaccggcctc
     5401 aaacgcaata tccgtcactg aatagttggt catttccagt tgttttttgg caaaattaat
     5461 acggatttca ttaataatct gcattggcgt tttgccataa tatcgttgag tcgcccgcgt
     5521 caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg attcactaaa
     5581 ctgctcttta tcatgcatct tttctaccgt acttttcagc cactgcggta catcatcaat
     5641 cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac
     5701 aaactcttcc agccctgttt cgcggaaatt caatgaagaa atcactgttt ccacataggt
     5761 gagaaaagcg ttattggtcc ggtatacctg cgaagctaca aagcaataag gcaacaatgg
     5821 caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc gcgtggcacc
     5881 aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat cgccccgttc
     5941 cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata ccagagtaaa
     6001 ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat agataaacac
     6061 atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt ccggcgcgtt
     6121 aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc tcaattaatt
     6181 cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg tggaccagca
     6241 ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc gtctgtacca
     6301 gatgcgcttc attcaatgcc atccgtgact gatccatcat ggcttttgct gcggcaaaat
     6361 cgccctgctt cgcctgtttc agtgcggcat acgccaggct gcgcgcttgt ccggagttga
     6421 tgataagccc catcaccact tcttccagct cttcagcttc cgtttgcgta tcgggaatat
     6481 tatcgagatc catcatacat cgttcctctc ttttcttacc ggcacgatta cccgtaccgg
     6541 catcgattaa aatttcaggg cgttagcgat atcttcttcg ctctcttctt tatcaatcgc
     6601 attttgtgct ttgttagcca ccacaacaaa gggcagataa attaacgttg cgataccaag
     6661 gttgaagagt gcaaccagca atgcgacgac gctaccgttg gtgttaaaga aggctcccag
     6721 accggttggc atggtccacg gtgcaatatt ggtcaccgga ggaataatgc ccatgtagta
     6781 cgctgccagg gtgattgccg ccagaatcgg ttgtaccagt acaaacggga taaacatcac
     6841 cgggttcatg ataattggca gaccaaacag aatcggttcg ttaatctgga agatgccgga
     6901 cggcagcgcc agttttgcca cctgacgata atcagcacgg cgagaggcaa taaagatagc
     6961 caggatcagg cctaaagtcg caccactgcc tccaaggaaa ataaaggagt ccagcatcgg
     7021 cttggcccag atatggaagg tcttaccggc tgccagcgcc gcttcgacgg aaccatattg
     7081 ctgataggtc gcgatatttt ccagcgccca cggcgtcata atgccgttgt ccagtgcggt
     7141 cagcgccagc gcgccatgaa taccgaagaa ccagagcagt ggaacaaaga tcacataggc
     7201 ccagcccacc acgctaccca acgatgccag tggggttgag atggtatcca taatgatctg
     7261 atggaagttg gtgccccagg tattcaacgc ccaggcaata atccccatca cggaaagaat
     7321 aataaagccg ggaattaatg ccgagaagga acgcgatact gaagcaggta cgctgtcggg
     7381 cagtttaatg acccaattgc ggcggacaat aaaggtaaac atttctgcca ccaccaggcc
     7441 aataataatc ccggagatga tattcgcccc acctaaccag tttgcgccaa ccgcataggc
     7501 ctcaccgaca ctatatgggg tgacggtcat aaatgctgca acggataaca acccagccgc
     7561 cagcgcatcg actttacgct cttctgccag cgccatgcca ataaagaacg gtgccattaa
     7621 agacattatc cctaatgttc cgttatatac gttgccgcca atacctttca gaccattaag
     7681 tgtttcaatg gttgaggcgt cgaggcgaat acctaaggaa taaaaaaacg acccctcccc
     7741 aaagcttaga aaaacgttgt taattaatac aaacatggcc cccgcaaggg ttaacggcat
     7801 taagcgaata aagccatttt tgattgcatt aacgtgtggc tgctttccta ttttaactgc
     7861 aaaagggagg agtacctttt caagcgatgc aataacatta ctcatagaaa aataccctta
     7921 aaaaccgcaa tttaaatatt gcggtattga tttatgaaat aactctttga cgggaaaatt
     7981 taaaataaat taatttgctg cggctttttt aatcgctgca accgcagcct taagcacgcc
     8041 taaaccatcg actttgccat aaagcagcga gtcaattact tcaaccggtt tgttgggtaa
     8101 caaacgctgg atttcgggca acatataagc aatctgcggc cctaataaca cgacatcggc
     8161 attctgacct ttttcaccag ccagtgtttc cggaaatgct tcaataatga ccggaacttc
     8221 atatttttct gcctgtgcgc gcatttttga taccagtaaa gaggtagaca tgcccgcaga
     8281 acaaaacaga taaatgtgtt tcttttccat aaaactgccc tcatcgacga ttatctgtca
     8341 gccagacact ccgcaagcct taacctgctt ccatgctctg ggtaacttgc gaaaccaaca
     8401 tgactttttt tgtggctgaa aggagtatac ggtatagacc tgagcaatag tatttctttg
     8461 accttataaa ttgtctctcc ttgacctgcg gttatgaccc tcttcacatt tcgggcaaat
     8521 aattcgcgaa gataattaag aactcaggcc aaaaaaaaac cggcgcaatg gccggtttcc
     8581 gttgttactc aagctttcag acgaagtgat tacttcgcag cctgtggatc agtgtcgtat
     8641 tcagcacagg tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag
     8701 taggtttctg ctttaccatc acgttgaccc aggatgtagg tctggcaagt accacgagca
     8761 tggatcatgc tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc
     8821 atgccttttt tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa
     8881 gccgtacaac ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc
     8941 ttgttcatat tccgtcctat tgtttatcag cgtgttagat aagcctggaa tacattgggc
     9001 gctttttcaa gcccatgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt
     9061 aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa
     9121 gttttgtctt ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc
     9181 tgggcgcaaa accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga
     9241 aaagctacct gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc
     9301 aggactccac gcttgccggt aagctgtgcc agatggcgat taatgagctg cgccaggaaa
     9361 ccggcaacga atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggccgacg
     9421 aacaagattg ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata
     9481 tcaagggcgc ggtattggct agcgagcagg cattgcggga agcatgcatt gaactgagcg
     9541 attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg
     9601 gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct
     9661 tcactaaata tggtgacggc ggtacggaca ttaatccact gtatcgtctc aacaaacgtc
     9721 agggtaaaca gttactggca gcattaggtt gcccggaaca cctttataag aaagcgccaa
     9781 cggccgatct ggaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct
     9841 atgacaatat cgacgactat ctggaaggga aaaacgtacc tcaacaggtc gccagaacaa
     9901 tagagaactg gtatctgaaa accgaacata aacgccgtcc gccaattacc gttttcgatg
     9961 atttctggaa aaagtaataa tttgcaccta ttggattgtg caaaaaagag taatttgttc
    10021 acgccggatg cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt
    10081 gcaggagtcg aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcga
    10141 cccgcctctt ttttaatcgc ttcccggctg ttacactgga tagataacca gcattcggag
    10201 tcaacagtgg tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat
    10261 gaatatcccg aacatttacg ttcattcctt aatgacttac ccacccgacc aggggtgtat
    10321 ctgtttcatg gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc
    10381 agtcgggtgc tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg
    10441 cgaattagct ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta
    10501 atcaaagaac aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc
    10561 ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga
    10621 gcccccaacc tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc
    10681 atcgctgatg aacaaaaact ttgttatggt ctgttgggac tggaaccgtt aagtcgcggt
    10741 cgtgcgtgtt ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc
    10801 catgaggaac atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg
    10861 ccttggcaag gggcggtggc gctgaaagaa cagcacccgg aaatgactca atatcatatt
    10921 attcaaaact ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt
    10981 cggacacccg ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc
    11041 ggtaactatg aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac
    11101 tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc
    11161 acgcgccctg gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa
    11221 acaccacccc gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg
    11281 ccttacagac atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca
    11341 gtttcgcttt taccacctgg tccgccgcaa aggcaaaagg ctgtaacggt tttaaggtat
    11401 ggttaaagtg gtctgcgctt tcaaggaaca tctcgccgcc ttccagcaac gtcactatcc
    11461 tttccatacc aggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac
    11521 gccagtaaaa atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag
    11581 cgtttcgcca caaattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg
    11641 gcccgttctt ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg
    11701 atttacgtta gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg
    11761 ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt
    11821 tggctgaaag attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc
    11881 agacgcttct caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa
    11941 attttgttct gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt
    12001 gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt
    12061 gcgcgacgtt cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg
    12121 cgaatctgct gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc
    12181 ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg
    12241 gtgtctgcgg catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt
    12301 gcagttaatt tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa
    12361 gtagcgtgcc gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa
    12421 cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa
    12481 tttgatggag agtgatgaga gaatattaca acaccatgat tttgcagaga ttatgaagaa
    12541 ctataccgga tgactggtga taaataaagc aaataaccag gattaatctg tattaattta
    12601 taagaaggta acttaatacc cgcagaaaga tttctgcggg taagtattag cttatttttt
    12661 cgagcattaa tcccgcgcgt aatcccagcg ctaccaacgg attagggaat aacacatact
    12721 ctacatcatg ggttacggta aaacgttcct caccgtcctg cgccagcaac gttcctttct
    12781 taaacggcat aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc
    12841 gagtaatttg cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct
    12901 caccagatag cagcgcagcg attgcgctgg cagttactgc aaactggcga agatcgtttt
    12961 gcccaaaggg caacgctttg ccaagttcca gcgtacaggc cagcgcgcca aaatgtctgg
    13021 cgctgaaatg ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg
    13081 ccgcacccag ccacgtcagg aatttctcgt cccagggaat gtcgcgttgc ggtaatacac
    13141 cgaactgcgg atgcaaggag ccacgaattg cggtatgtag atcaaggtgc cagcgcacag
    13201 attctttgcc ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg
    13261 tttccccgct ttcagcaaac agctgccaac gaccgccaaa cattcggttc atatcgctat
    13321 ggcaataacg tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc
    13381 accgtaacgg gatctcgccg tgagatatcg cgccaagcaa tgcatcaagc atctccacag
    13441 gtgccgtctc attaccgtgg atccccgcag aaatcaccag agcgccttgc ggtggcgtta
    13501 atggcgtcag ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc
    13561 gctcggtgat gacaggtttt ttactcgtta aggtcagagc aagaaaatta tccattgccc
    13621 cctccctcgc gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc
    13681 agtgcttccc gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga
    13741 tcgcggtagt aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc
    13801 accgccggat tcaccgcccg gcgttcttct tgtgtcaata ccacccgcaa ccgcaggcac
    13861 gccgggccac cgccattcgc catgctttca cgtaaatcaa agacttttag ttcgctaatc
    13921 gggttgtcag cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac
    13981 tcctgaggca gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac
    14041 agatatgtag acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata
    14101 aaaccgttca cccgcgcacg caggtttgcc agtaactgtg actggcgagc gaacgcctgt
    14161 tggtggcaaa acagcacctg gcggttactc acggcaatca cgtcattatg aaaaacgccc
    14221 tggtcgataa cgtccgggtt ttgctgggcg aaaatcacct gttggggatt cacctgattc
    14281 agccttgcca ccgcctcgct ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg
    14341 gtatcattgc cctcttctcg cccgtagaca aaaagttgca tacccggttc accgtaatga
    14401 ccgccgagac gattgtggtt tgccgccccc tcatcaccga gcaaggctac ctgcggcaac
    14461 gccgaatgga cactaaattt ctcttcgtcg ttaaaaatcg cttttaacag cgattcagtg
    14521 acgggcgctt ccagcgaacg gtgaaattta ttgttcaggt tagcaacggt gagatgcact
    14581 ttgccatcca gcgtatcggc agatggcgcg atcgttgccg cattggctac ccacattggc
    14641 gaagcggaac tgacgctgga aagccagtgc ggtgcctggc gtacaacctt ttccagtacc
    14701 tgctcatcgc taccactgaa tcccaactga cgcagcaccg gaataaacgg acgctcgtga
    14761 ggcgggatca cggcctgggg gaatcccgca tcggcaaggt ttttcatttt cagtaagccc
    14821 tgttttgccg ccagtcgcgg gttagagacc tgaaaacggt gacgggtaga ggctttatta
    14881 ccaaacgaca ggcccgcgta atgatgcgtc agccctacca gcccgtcgaa attaacttcc
    14941 caggcgttca tcgcaccacc tcatcggaaa aatccagccc ggggttaagc gttgcgggca
    15001 aagttaacga gtccgactcc aggctcgcca taggccatgc gcagtaatct gcggcatacc
    15061 aggcgctggg gcgatggtta ccggatgcac caatgccgcc gaatggcgcg gtactggcag
    15121 caccagtaag cggtttgttc cagttaacaa tccccgcccg cgcctccagc aacagttgat
    15181 cgaatttttc ccgctcgggg gaaaccagac cgcaagagag tccgaagcga gtgttattcg
    15241 ccattcgaat cgcttcctcg aaagaatcat aacgccagac gcgcaataac ggtccgaaca
    15301 cctcttcatc tggtacgcca gcaacgcctg tcatttcaat gatccccggc gtcagcaacg
    15361 atgtccctgc ttgtaataag cgcggcgcaa gcagggttcg tccacccatc gcttccagtt
    15421 gctgccaggc agtaaacacc tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg
    15481 gctgcggttc gtcatcccag ttgcccggcg ttaatcgctg gctgacggca accagacgag
    15541 caagaaatgc atcgccctgc gccccgcttt tcagcaatac acggcgggcg caggtgcagc
    15601 gttgcccggc tgtaacaaac gccgactgaa tggtcagatg gacagccgcg tcgatatccg
    15661 ccacctcatc gataatcagc gggttattgc cgcccatctc aagggcgaga attttctccg
    15721 gctgaccgga gagctggcga tgcagctggt agccggtatt ggcgctgccg gtaaacagca
    15781 agccgtcgag atcctccagc gcactcagcg cctgacccgt ttcacacccg ccctgcacca
    15841 ggttcagcac gcctggcggc aagccagcct gctgccataa acgcattacc gcttcgccac
    15901 tccacggtgt cagttcgctg ggtttaaaga taatggtgtt acctgccagc aatgcgggaa
    15961 cgatatgtcc gttcggcaaa tgaccaggga aattatacgg cccaaacacc gccagcacgc
    16021 cgtgcgggcg atgtcgcagg ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg
    16081 tacgaacgtg atacgcctta attgatatcg cgattttatt gatcatcgcc gtcaattcgg
    16141 ttgccgcctc ccagcgcggc ttgccggttt ctcgggcaat gattgcggtt aattcgcctt
    16201 tattcctttc cagcaatccg gcaaagcgtt cgacaacggc ctgacgttca gcaaatgaga
    16261 gccgcgccca gcgcggaaac gccgcacggg ctgcccgaca agcctgcccg acctgagcgg
    16321 catcggcatc attgccttgc cataacacct cgcccgatac cggattacgc ttcacacgcg
    16381 atgcaccctg gcccgttatc cagtcaccgt taatccataa agtcatgctg ttttctcctc
    16441 tgcgcacagg cgcaccagac gaacgcgatc ccctgcatgg catttgaggg catccagttg
    16501 tgcggcggtt aaaatcaaac gctcggttgc cggatcggta cgcaccagca ccacgcggaa
    16561 atggtgataa ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg caggctgccc
    16621 ttctgccact tccaccagcc gacttttacg gatggcgcgc acgcggtcga tgtcacactc
    16681 aagcgtcggc ccaccgtcga agatgtcgat atagttgcgg tagcgaaaac cttctttctc
    16741 cagcaccgcg cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt cctgggcttc
    16801 ctgggataaa aagtgcgtat agatcggatg tttcggcatc agttctgcaa taaatgcctt
    16861 ttgcccggtg ccgcagagaa aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc
    16921 gaggctttgc cagaacggtg aatagccgtg ttcgtcaatc accccgcgca tttcagcaac
    16981 cactttgtca ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg atttcgacag
    17041 caaatagccg ttgccctctt tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct
    17101 gctgccggtg tgatcgttac tgagaaacaa cgtcggcaat gcgttataga cattcagctc
    17161 ttttgaggcg tgaaccaacg tgccgacgcg atagttgtac cagggatcgt tcagcccaac
    17221 tgccacctca atggcgcaaa tccccgccac ggtgcctgtc tcgctatctt ccagtacaaa
    17281 cacataaccc tgctcacttt tgggcagttc accttgccag gttttgattg ccctttcgat
    17341 acgcgccgaa agcgtggctt cattggcggg aagcgacgtc aggccgccgc ccgttttgct
    17401 ggcaagctgc atcagcgccg agacatctga gcgctcaacg ggacggatga ccatcatgat
    17461 gaacctcggc taacaaagtg ttcgcaagca actgcaaagc gatccagtcc ggtcgtcacc
    17521 tcttcttcgc tgacattgag cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt
    17581 accatcacgc ctgctttcgc cgcttcctga gagatctgtt tcgcttgccc ggcgtaatcg
    17641 gcattcagta cacagccaat cagcaaacct aagccgcgaa cttcattgaa caaaccatag
    17701 cggtgattaa taatattaag acgctcaaca aaccagtcgt gacgttgctt aaccccgtta
    17761 agcatctctg gcgtattgat gagctccagc actttgcctg ccaccgccga ggccagcgga
    17821 ttgccgccat aggtggtgcc atgagtgcca acggtcatca cgctggcgca ctcttcggtt
    17881 gctaacaacg caccgaccgg gaaaccgccg cccagagctt tggcggtagt taacagatca
    17941 ggcgtcacgc cgtagtgcat ataggcatac agttccccgg tacgcccgac gccggtttgt
    18001 acttcatcaa aaatcaacag cgcattgtgg cgatcacaca attcacgcag accttgtaaa
    18061 aatgcgttgc tggctggcac cacaccgcct tcaccctgga tgggttcgac aatcaccgca
    18121 caggtagagt cgtcaatcag cgcgctggca gagttaatat cgttatatgc agcatgacgg
    18181 atatctggtg gcagtggcgc aaaatcctgt gaataggctg gctgcccacc cgcactaaca
    18241 gtaaacagcg tgcgaccatg aaacgcattt ttaaacgcca cgataccgct cttatggctg
    18301 ccgtagcggt cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca
    18361 ccggagttac aaaagaaaac gcgatcggca aacgtggcgt cgatcaattt tttcgccagt
    18421 cgcagtaccg actcgttggt gtaaccgttg ccggtatgcc agaacttact cgcctgttcg
    18481 ttcagcgcct cacgcagttc cggatgcgca tggcccagcg cgttcaccgc aatgccaccc
    18541 gcgaagtcga tatactcttt cccctgctga tcccacaagc gcgaaccttc gccacgtacc
    18601 ggtataaacg gtgccggagc gtaaacaggt atcatccatt catcaaagtt ttcacgcgta
    18661 attggctgag acatagcgac ctctacagta aatagctcgt tatttatatg ttaataataa
    18721 gtaatgtttt cgctgtaaat gtagattgca gggttcgtgc cagccagtga tgaaagtgca
    18781 taaacggcgg aggctaactg gaaatcaagg agttataacc aaaccatatg catttaaagt
    18841 gcatattaag tgaatacgtt tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt
    18901 atgcagaagt aaaatataat tctggaattg tgatcattga cgaaatttac tggaaattac
    18961 tgcgccattc tgacgcagcg cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc
    19021 attaacaacc atcgatcaaa tcacttaaca acaggcggta agcaaagcga aattctgcta
    19081 ccatccacgc actctttatc tgaataaatg gcagcgacta tgaaatttgt ctcttttaat
    19141 atcaatggcc tgcgcgccag accccaccag cttgaagcca tcgtcgaaaa gcaccaaccg
    19201 gatgtgattg gcctgcagga gacaaaagtt catgacgata tgtttcccct cgaagaggtg
    19261 gcgaagctcg gctacaacgt gttttatcac gggcagaaag gccattatgg cgtggcgctg
    19321 ctgaccaaag agacgccgat tgccgtgcgt cgcggctttc ccggtgacga agaagaggcg
    19381 cagcggcgga ttattatggc ggaaatcccc tcgccgctgg gcaatgtcac cgtgatcaac
    19441 ggttacttcc cgcagggtga aagccgcgac catccgataa aattcccggc aaaggcgcag
    19501 ttttatcaga atctgcaaaa ctacctggaa accgaactca agcgtgaaaa tccggtgctg
    19561 attatgggcg atatgaatat cagtcctggc gacctggata ttggcattgg cgaagagaac
    19621 cgtaagcgct ggctgcgtac cggaaaatgt tctttccttc cggaggaacg cgaatggatg
    19681 gagagactga tgagctgggg attggttgat accttccgcc atgcgaatcc gcaaacagca
    19741 gatcgtttct catggtttga ttaccgctca aaaggttttg acgataaccg tggtctgcgc
    19801 atcgacctgc tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac
    19861 tatgaaatcc gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc
    19921 cgctaattta gcagctctcc tggctcaaac tgggtcagga gaattaacct tgagaaaaat
    19981 caacaaactg tcagtaatga tttgttgcct gccgtccttt gttataccgt cactgcgttt
    20041 ttagttgtct gaccacttct ctattatcaa gtttgatata ggaaactcca cgatgaacgc
    20101 tgagcgtaaa tttctttttg cctgtcttat ttttgcgctg gccatttacg ctatccacgc
    20161 tttcggttta ttcgatctgc tcaccgattt accccactta cagacactca ttcgccagag
    20221 cggattgttc ggttatagcc tctatattct gttattcatc attgccaccc tctttctgtt
    20281 accaggaagc atattggtga tcgcgggagg aatagttttt ggcccgttct tagggacact
    20341 actctcatta attgccgcca cgctggcctc ctcgtgctca ttcctgctgg cgcgctggat
    20401 gggacgtgat ttactgctga aatacgttgg ccatagccat accttccagg ccattgaaaa
    20461 aggcattgcg cgtaacggta ttgattttct tattctgacc cgcttaatcc cgttgtttcc
    20521 ttacaatatt caaaattacg cttacggatt aaccacaatc gccttctggc cttataccct
    20581 tatttcggca ctcacgaccc tgcccggtat tgttatttat accgtgatgg caagcgatct
    20641 cgccaatgaa ggcattacgc tgcgctttat tttacaactc tgtctggcgg gcctggcgct
    20701 gtttattctc gtccagctcg caaaactcta cgcccgacac aaacatgtgg atctgtctgc
    20761 ttcgcgccgc aacccactta ctcacccaaa aaatgaagga tagaacgatg ttgcaacatt
    20821 attcagtgtc atggaaaaaa ggactgactg cactctgctt actggctgtt gctgggctta
    20881 gcggctgcga tcaaaaagag aatgcggcgg caaaagtgga atacgacgga ctttcgaaca
    20941 gccaaccgct gcgcgtcgat gccaataacc atacggtaac catgctggtg caaattaatg
    21001 gtcgtttcct caccgacgac actcgtcacg gtattgtgtt taaagatggc tccaacggac
    21061 ataaatcgct gtttatgggt tatgcgaccc cgaaagcatt ttatgaagcc ctgaaagagg
    21121 caggtggtac accgggcgaa aacatgacga tggataataa agaaacgact catgtcacag
    21181 gcagcaaact ggatatttcg gtcaactggc aaggggcggc aaaaacgtat tccttcgatg
    21241 aagtgattgt tgacagtaat ggcaagaaac tggacatgcg ctttggcggt aatttaacgg
    21301 cagcagaaga gaagaaaacg ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg
    21361 tcagcaatgc aacatacact tatggtgcgg ttgaaaaacg aggtgaagtt aaattcaaag
    21421 gcaatgcctc ggttctcccg gcgaataaca cgctggcaac ggttaccttt aaaatcaccg
    21481 aataaagcca ggataaagga tgatgaagat gcaatcgcgt aaaatctggt actaccgtat
    21541 aaccctcatc atcctgttgt tcgctatgct gctggcatgg gcgctgcttc ccggcgtcca
    21601 tgagtttatc aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg
    21661 ctttattcag tcttacggcg cactggcggc agttgtctcg ttcttgttga tgattttaca
    21721 ggccattgct gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg
    21781 cgcgttctgg ggcggctcgc tgtcgtggac cagttcgatg gccggcgcgg cactgtgctt
    21841 ttttatcgcc agagtgatgg gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtact
    21901 tgacagtatg gacggctttt tcactcgcta cggcaaacac accatcctgg tctgtcggtt
    21961 attgcctttt gtccctttcg atccaatcag ctatgctgcc ggtttgactt caatacgttt
    22021 tcgctcgttt tttatcgcca ccgggcttgg tcagttaccg gcgactattg tttattcctg
    22081 ggcgggcagc atgttaacag gcggtacctt ctggtttgtc accggactgt ttattctgtt
    22141 tgccctgacc gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa
    22201 tgcctgatgg gtttaccgcc gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg
    22261 ctgcatcgtc gccattacgg cgaggtgcta agccccattc gctggtgggg gcggatcccg
    22321 tttatctttt atctggtgtc gatgtttgtt ggctggctgg agcgcaaacg ctcaccgctc
    22381 gatccggtag tacgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt
    22441 tgtgtggata tcaccagtat gaaagtcgcc gagcgcaccg gcagcagcga taaactgctg
    22501 gcagtggctg actggcggca aagcccgctc tttagcgatg aagaacggct ggcgctggag
    22561 tacgccgaag ccgcaagcgt aacgccgcca acggtcgatg atgccctgcg tacccgactg
    22621 gctgcgcatt ttgacgctca ggagctcacc gaactgacgg cattgatcgg cctgcaaaat
    22681 ctgtcagccc gttttaattc tgccatggac attcccgctc aggggctgtg ccgtattccg
    22741 gaaaaacgtt cttaaggaga gatgatgcgc cattgtgggt ggttgctggg attgttatcg
    22801 ctgttttctc tggcaacaca tgccagtgac tggcaagaaa ttaaaaatga ggccaaaggg
    22861 caaactgtct ggtttaacgc ctggggcggc gataccgcaa ttaaccgcta tctcgactgg
    22921 gttagcggcg agatgaaaac ccattacgct ataaacctga agattgttcg tctggcggat
    22981 gccgcagacg cggtgaagcg cattcagacc gaagctgctg ccggacgtaa aacgggcggc
    23041 tcggtggatc tgctctgggt gaacggcgaa aacttccgca ccttaaaaga ggccaattta
    23101 ctgcaaaccg actgggcaga gactctgccc aactggcgct atgtcgacac acagctgccg
    23161 gtgcgggaag atttttcagt gcctacagaa ggggctgaat cgccctgggg gggcgcacaa
    23221 ctgacattta tcgcccgccg cgatgttacg ccacagccac cacaaacgcc gcaagcctta
    23281 ctggagtttg ctaaagccaa tcccggcacg gttacctatc cgcgcccacc ggactttacc
    23341 ggcacggcgt ttcttgaaca gttgctgatt atgctgacgc ccgatcccgc cgcattaaat
    23401 gaagcgccga acgatgcgac tttcgcccgt gtcactgctc ccttgtggca atatcttgat
    23461 gcgctgcatc cgtatttgtg gcgcgaagga aaggatttcc cgccttcgcc cgcgcggatg
    23521 gatgctctgc tgaaagccgg aacatttcgc ctgtcgctga cctttaaccc cgcgcatgcg
    23581 cagcaaaaaa tcgccagcgg cgatttgcct gcaagcagtt acagttttgg ctttcgcgag
    23641 gggatgattg gcaacgtgca tttcgtcacc attcctgcca acgcgaatgc cagtgctgcg
    23701 gcgaaggtag ttgccaattt cttgctctca cccgatgcgc aattgcgtaa agcagatccc
    23761 gctgtctggg gcgatccttc tgttctcgat ccgcaaaaac tgcctgatgg gcagcgcgaa
    23821 acattgcaat caagaatgcc gcaagatctg ccgccggtac tggctgaacc gcacgcaggt
    23881 tgggtaaatg cgctggaaca agaatggcta caccgttacg gtacgcatta atctttttgc
    23941 tgtgggcgat ggtggcggtg atttatgcac cgctgatccc ggcagctctc acgctgatat
    24001 cgcctgcttt gtcgttgaca cactggcagg cgttatttgc cgatccacag ttaccgcagg
    24061 cattactggc aacgctggtg tcgacaacca tcgcggcggt cggggcgttg ttgattgccc
    24121 tactggtgat tgtggcgctg tggcctgggc cgaaatggca gcgtatgtgc acccgtctgc
    24181 cgtggctgct cgccattccc catgtggctt ttgccaccag cgcccttctg ctctttgctg
    24241 acggagggct gctttatgac tatttcccgt atttcactcc gccaatggac aaattgggta
    24301 ttgggctggg cttcacgctg gcggtgaaag aaagcgcatt tctgctgtgg atcttagcag
    24361 cggtattgag tgaaaaacgg cttttacagc aagtcatcgt gctggattcg ctgggctaca
    24421 gccgctggca atgcctgaac tggctgctgt tgccctccgt cgcgcctgcg ctggcaatgg
    24481 cgatgctggc gattgttgcc tggtcgctgt cggtcgtaga tgtggcgatt attcttgggc
    24541 caggtaatcc accgacgctg gcagtaatta gctggcagtg gttaactcag ggcgacgccg
    24601 atcaacaaac gaaaggcgca ctcgccagcc tgctgctgat gttgttactc gccgcctacg
    24661 ttttgctggg ctatctgcta tggtgtacct ggcggcgtac tattccccgc gtagatggcg
    24721 ttcgtaagcc tgccacgcct ttattgccag gcattacgct ggcgagtttt ttacccttga
    24781 ccagtgtgct gtgtgtggtt ctgctggcga tcctcgcgga tcagtcgacg atcaatagtg
    24841 aagcgctcat caacagcctg acaatggggc tggtggcgac attcatcgct ttgctcctgt
    24901 tactgctgtg gctggaatgg gggccacagc gtcgccagtt gtggctatgg ttgccgattt
    24961 tattaccggc actgccgctg gtggcgggcc agtacacgct ggcgctatgg ctgaacatgg
    25021 atggaagctg gacggcggtg gtctgggggc atctgctgtg ggtaatgccg tggatgctgt
    25081 ttatcctgca acccgcctgg cagcgcattg attcacggtt aattttgatt gcacaaacac
    25141 tgggctggtc gcgggccaaa atcttctttt acgtgaaatg tccgcttatg ctacgcccta
    25201 cgctgattgc cttcgcggtg ggattttcag tcagtattgc gcagtatatg ccgacgctgt
    25261 ggctgggcgc ggggcgtttt ccgacgctca ccactgaggc ggtggcatta agcagcggcg
    25321 gcagcaacgg tattctcgcc gccctggctt tatggcaact gctattaccg cttattattt
    25381 ttgccctgac cgcgttagtc gcaaaatggg taggttatgt cagacaagga ctccgctaat
    25441 gctctgcgtg aaaaatgttt cgctacgttt accagaaagt tgcttgctga caaacgttaa
    25501 ctttacggtg gataaaggtg acattgtcac gttaatgggg ccgtctggct gtggaaaatc
    25561 cactctgttt tcatggatga ttggtgcact ggccggacag ttttcttgta caggtgagct
    25621 atggctcaat gagcaacgga ttgacatgct acccaccgca cagcgtcaga ttggcattct
    25681 ttttcaggat gcactgttat ttgaccagtt cagtgtcggg caaaatttac tgctggcgct
    25741 accggcgaca cttaagggga atgcccgacg taatgccgtg aatgatgcac ttgagcgttc
    25801 aggccttgac ggagctttcc atcaggatcc tgctactttg tctggcggtc agcgagcgcg
    25861 cgttgctctg cttcgcgccc ttctcgctca gccaaaagcg ttgctactgg atgagccatt
    25921 cagccgtctt gatgtggctc tgcgcgataa ttttcgccag tgggtgttca gcgaagttcg
    25981 cgaactggcg atccccgtcg ttcaggtaac gcacgatctc caggatgttc ctgctgatag
    26041 ttctgttctg gatatggcgc agtggtcaga aaattacaac aaactgcgat aacgcaaagt
    26101 ttttctcaat gcgtcagttc agaatggcgc actcaaaact acaatgtcgg gattttcgat
    26161 gaaacgtgtt tctcaaatga ccgcgctggc aatggcttta gggctggctt gcgcttcttc
    26221 gtgggccgct gaactggcga agcctcttac acttgaccag cttcaacaac aaaatggcaa
    26281 agcgatagat actcgctcca gcgcgtttta taacggctgg ccacaaacct taaatggccc
    26341 ttctggtcat gaacctgccg ccttaaacct ctctgctagc tggcttgaca aaatgagcac
    26401 cgaacagctc aacgcgtgga tcaagcaaca taacctgaaa gccgatgccc cggtggcgct
    26461 gtacggtaat ggcaaagatg tcgacgccgt caaaacgcga ctgcaaaaag caggttttac
    26521 gcatatctcc attctgagtg acgcgctaag cgaaccttcc cgtctacaaa aactgccgca
    26581 ctttgagcag ctggtttatc cgcaatggct gcacgacctg caacaaggta aagaggttac
    26641 ggcgaaacct gccggtgact ggaaagtcat tgaagcggcc aggggcgctc ctaagcttta
    26701 ccttatcagc catattcccg gtgctgacta catcgatacc aacgaagtgg aaagcgaacc
    26761 gctgtggaac aaagtttctg atgaacaact aaaagcgatg ctggcaaaac acggcattcg
    26821 ccatgacacc acggtcattc tgtacgggcg tgacgtatac gctgcagcgc gtgtggcgca
    26881 gattatgctt tatgccggcg tgaaagatgt gcgcctgctg gatggcggct ggcaaacctg
    26941 gtccgacgcg ggtctgcccg ttgagcgcgg tacgccaccg aaagtgaaag cggaaccgga
    27001 tttcggcgtg aagatcccgg cacaaccgca gttgatgctc gatatggaac aggcccgtgg
    27061 actgctgcat cgccaggatg catcgctggt gagcattcgt tcgtggccag aatttatcgg
    27121 tacgaccagc ggttacagct atattaaacc aaaaggtgaa atagccggag cacgttgggg
    27181 acacgctggt agcgactcga cgcatatgga agatttccat aatccggatg gcaccatgcg
    27241 tagcgccgat gatattaccg ctatgtggaa agcatggaat atcaaaccag atcagcaagt
    27301 ttcattctac tgcggcaccg gctggcgcgc gtccgaaacc tttatgtacg cacgagcaat
    27361 gggctggaat aacgtctccg tttacgacgg cggctggtac gaatggagca gcgatccaaa
    27421 aaatccggta gcaaccggtg aacgcggccc ggacagcagc aaataacatt gcaattactg
    27481 acgctggagc gacttcagcg tcagataccc actccacacc cgcgtaaagg tcgtcatcca
    27541 gcacaacgca ccgaagatcc acgcaaacca cgcaaaccat gcagggaata aacatcccag
    27601 cacaaacagt aagatcgttt ctgtgccttc ggttaatccc cccagataat aaaacgattt
    27661 atgcgcataa ccgggattat caatctgata tttcgccgcc agcgcagcaa aagcgagaaa
    27721 actgctaccc gtgccgataa acgcaaacag cagccagccg cctgccaggg cgttttgctg
    27781 cggtgcggcg aggataaacc caaaaggtac cagcgcgtaa aaaagaaaat cgagagaaat
    27841 atcaagaaag ccgcccgcgt cggtaagccc tctgcgccgc gccagcgcac cgtccagacc
    27901 atcaagcagc ctgttcaaca aaatgacgac aagcgccgcc agataccagc ccagcgccag
    27961 aaaaggcagt gccagcacac cgatggcaaa tcccactaat gttaaaccat ctggcgtaat
    28021 gcccggttta tcaagaaccc gcacacactg atgcaacaac ggtttaatcc ggggatgaag
    28081 atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag caacagtgcg
    28141 gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg attgaagttg
    28201 ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc gcccagagcg
    28261 atcaggcggg attatgggag tttgccggtg gtaaagtcga gccggatgaa agccagcagc
    28321 aggcgctggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg ggtgagtatg
    28381 ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc tggcacgtac
    28441 ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg tgctcacctg
    28501 aagaggcgct gcaatatccg ctggctcctg ctgacattcc attattagag gcgtttatgg
    28561 ctttacgcgc cgccagacca gcggattagt gctaagggtt ttgtcatccc gctggcattg
    28621 cagcagtatt ccttcggctt taattaccgc cccttcagaa taatttttat cctgataaac
    28681 gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaccactt ctggcggtac
    28741 gtttacctcc acgtccggac cataatgcgg gttagccagt gcaattaatg gaaatgctaa
    28801 tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt ctctgctact
    28861 gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg gttatgagat
    28921 tactctcgtt attaatttgc tttcctgggt catttttttc ttgcttaccg tcacattctt
    28981 gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca caatcgtatt
    29041 aatatataag ggttttatat ctatggatca gacatattct ctggagtcat tcctcaacca
    29101 tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac
    29161 cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat tactggagcg
    29221 tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg atcgcaatca
    29281 ggtacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc cgtacaaagg
    29341 cggtatgcgc ttccatccgt cagttaacct ttccattctt aaattcctcg gcttcgaaca
    29401 aaccttcaaa aatgccctga ctactctgcc gatgggcggt ggtaaaggcg gcagcgattt
    29461 cgacccgaaa gggaaaagcg aaggcgaagt gatgcgtttc tgccaggcgc tgatgactga
    29521 actgtatcgc cacctgggcg cggataccga cgttccggca ggtgatatcg gggttggtgg
    29581 tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata ccgcctgcgt
    29641 cttcaccggt aagggccttt catttggcgg cagtcttatt cgcccggaag ctaccggcta
    29701 cggtctggtt tatttcacag aagcaatgct aaaacgccac ggtatgggtt ttgaaggcat
    29761 gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga
    29821 atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg
    29881 attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa tccagccgcg atggtcgagt
    29941 ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt
    30001 accggttgat atcgccctgc cttgcgctac ccagaacgaa ctggatgttg acgccgcgca
    30061 tcagcttatc gctaatggcg ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat
    30121 cgaagcgact gaactgttcc agcaggcagg cgtactattt gcaccgggta aagcggctaa
    30181 tgctggtggc gtcgctacat cgggcctgga aatggcacaa aacgctgcgc gcctgggctg
    30241 gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc accatgcctg
    30301 tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga acattgccgg
    30361 ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg taaatgcctg
    30421 atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta cgctctaatg
    30481 taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt atttaacgga
    30541 tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt agtgctccca
    30601 aaactgcttg tcgtaacgat aacaagcttc aagttcagca tccgttaact ttctgcgata
    30661 gcagcagata tgccagtaaa gaaatcccat tagactattt ttttgataat cttcttcgct
    30721 ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc
    30781 ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag atcatccatg ataattccgc
    30841 cgcccatatt agcttcgccg aggatttacc ggagctatga ttagcgcaat cagagatata
    30901 gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc
    30961 catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaaggc
    31021 acaataactt tcgctatctt cgatacccca ttgttcctct aaagactcgc gtcttttact
    31081 tatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt tgccaggttc
    31141 aggctttcgc ggccagaact ccagcgtttc agaccattgc ttatgataga atcgataagg
    31201 tgcgatcaat tgtagcgcct gtaatttctc gatactgagc ggctcaatac ctttagcctg
    31261 ataataatgc agctgttctt tttttgcttt aaaaccggcc cgaacaataa gccccatcat
    31321 aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat tcaaaccgtt
    31381 ggatgttatc gctgcgaata caaacattac agcgacaaca catgttaaat aaaaccccca
    31441 cttacaaagc agcatggtct tattttcttt aatcatccgt tcaaaattac tattaaatat
    31501 ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata aaactttttt
    31561 catcacattc cctgttatta catactttag aggcgataat tttatccatg caaaaaaaat
    31621 atccaacgaa ttttcttgat ccgctggata tttcagaata tgactcgaat agcacgaaag
    31681 attcgctcgc ttacgctatc gccccgcttc cggcttcatc tgctggcgga ctttttttcg
    31741 cactacgttt acgcggtgca gcctttttct tatcagcact gccaccactg cccggagcca
    31801 caatgccgcg aaactgccgc accggagtac gtttggcttg atcaataagc tgatatagcg
    31861 tccccaccag cggctgcata aagtcctgat agcgacactg cttttcgctg atttgcgtca
    31921 gcaccgattc ccagtgcgcg gtcatgtccg gtcgcgtagc catttctggc agcgaatgga
    31981 ataacgcttt tccggcgtcg gtggagtgga tatagcgccc ttttttgatg aggaaaccac
    32041 gcttgaacaa cagttcaata atcccggcac gcgttgcttc tgtccccaga ccatcggtcg
    32101 cacgaaggat ctttttcaga tctttgtcct gcacaaagcg cgcgatcccg gtcatcgccg
    32161 aaagcagtgt tgcatcggta aaatggcgcg gcggctgggt ttgccgctct accacttcac
    32221 ctttttcaca cagcaactca tcgcctttcg ccaccacagg caatggcgta ccgtcgtttt
    32281 cttcatcgcg ctctttgctg cctaacagcg tgcgccagcc tgcttcagca agaaaacgcg
    32341 ctttagcgac aaatttgcct ttggcaatgt ccagttcgat aacacacttg cggaacaccg
    32401 catccgggca gaactgcatc agatactgac gggaaatcag gttatagacc ttcgcttcgt
    32461 tctccgtcag gttgatcgca gaactccgtg cggtcggaat gatggcgtgg tgcgcatcga
    32521 cctttttgtc atcccaacag cggttgcgta tatctggatc taccactggc tgcggcaaca
    32581 gatctggtgc atgaacgctg atggcattca tcaccgcgtg gcgtccggca aaatgttctt
    32641 ctggcaaata gcgacaatcg gaacgcggat aagtgattag cttgtgcgtt tcgtacagct
    32701 tctggcagat atcaagcacg ttctgcgcgc tcagaccaaa gcgttttgcc gcttcaatct
    32761 gcaacgccga aagcgaaaac ggcagcggcg cggattctga ttcccgttta tcgttatagc
    32821 tggtgacaat agccggttga ccactaatgc ggttaaccac atgctccgcc agtggacgat
    32881 gtaacaagcg cccttcttca tcctggtacg gttcacacgc ttcgctcggt tgccagatag
    32941 cggtaaaccg ctcatcggca ggtgtcacga tatgtgcttt gacttcaaag aagtctttcg
    33001 ccacgaagtt ttcaatctct tcatcgcggc gcaccaccag cccaagcacg ggcgtctgca
    33061 cgcgtcccac ggaaagcacg ccctgataac cggcattgcg accgagaatg gtatacgcac
    33121 gggtcatatt gatgccgtac agccagtcgg cacgcgctcg cgccagcgca gaaacgcaca
    33181 gcggtacaaa ctcactgttg gaacgaagac ggtcgatcgc ccgctcaacc gcctgcgggt
    33241 tcaggtcgtt tatcaagcaa cgctgcacct gctggcgctt ttccggtgcc agttgtagat
    33301 agtccagcac ttcatccacc agcagttgcc cttcgcgatc cgggtccccg gcgtgaacga
    33361 tttcgctggc ttcatgcagg taccgtttga tgacgttaag ttgtttggtc acggagggtc
    33421 ggggctgtaa ttgccacttt tccgggacaa tcggcaaatc cgcaagattc cagcgcgcat
    33481 agcggctgtc gtaggcgtct ggctgcgcct gctcaagcag gtgaccgata caccaggtca
    33541 ccacctgacc attaccgcac tcgataaagc catcgccttt ccggtgcggt ttgggcagga
    33601 catcagcaat ggcgcgcgcc agactcggtt tttcggcaat aaacaaccgc attgaattaa
    33661 cggatctcaa ccatggcacg accgccgcgt gctggcacca gttcgccaat cgccgtcagt
    33721 tcaatgccaa actcggcggc tgtagctttg acctcatttt cggcttccgg catgaccgcc
    33781 agcagcaaac cgccagaagt ttgcggatcg cacagcaaat cgcgcacttc acgcggcatt
    33841 tcacccatca gatgaccgta gctggcaaag ttacgttcgg tgccgccagg cactgcaccc
    33901 aacttaatgt actcttcaac ccccggcaat ttcgggatcg cgtcatagtc aacgcgcgcc
    33961 tgcacacctg cgccctgaca catttcgctc aaatggccca gcaggccaaa gcccgttacg
    34021 tcggtcatcg cttttacgcc ttcaatgttg gcaaaggacg cgcctgcgat gttcatccgg
    34081 cacatcactt ccgtcgccag tccctgatgt tctggtttca gcagtgattt tttctcagcc
    34141 gtggtaagaa cgccgatccc cagcggtttc gtcaggaaca gtttacatcc ggcttgtgcg
    34201 gtactgtttt tcttcacccg ctcggtcggt acgatccccg ttaccgccag accaaaaatc
    34261 ggctccggcg catcgatgga gtgaccgcca gccagcgcaa tacccgcctg acggcatgca
    34321 tagcgtccac cttcggttac ttcgcgggca atttccgggg aaagtttgtt aatcggccag
    34381 ccgaggatcg caattgccat aatcggtttg ccccccatcg cgaagatatc gctgatggcg
    34441 ttagtcgccg caatgcggcc aaaatcgaaa ggattatcaa cgatcggcat aaagaagtcg
    34501 gtggtactga taacgctggt gccattgccc agatcgtaca ccgccgcatc gtcgcgggtt
    34561 tcattaccca caagcaaatt cggatcaaca aacttcgcct gctcactgtg caggatggtt
    34621 tccaacactt ttggggaaat tttacagccg caaccagctc cgtggctgta ttgggtcaaa
    34681 cgaatcgagt tctcgctcat ggacatctcc tgtcaatgca atccgggtat ggtaaccctc
    34741 attccgtgaa gtgataagtg agagtgtctg aattcctgcg cctttgctca caatccagac
    34801 agtttcgcga caattatcag aaataagtga caaacggcgt cgggtccggg acgtttatcg
    34861 acgtagatgc tttcagctgc ggcgtgccga ggtagagaaa accgacaatt ttatcctgct
    34921 cacggcaacc gaatgcttca cgcactaccg gactttcagt taatgcgcca ctgcgccaga
    34981 tgccgccaaa cccctgggca acggctgcca tttgcatcgc catgaccgcg catccggcag
    35041 acatttcctg ttcccatcgc gggactttat gattttcttc gcatttcgcc accaccgtga
    35101 tgatgagcgg tgcgcggaac ggcgcattac gggctttgtc gatagctttg tcatcactac
    35161 cggcagcaat cgccccctgt tccagtacgg cgctgaaacg ctcgcgccct tccccttcaa
    35221 tcacaaaaaa atgccacggt tgcatggact tatggtccgg cgcgcgcata cccgcacgca
    35281 ggatgttttg cagttgttca cccgttggtg cgggttcagc caggcgggag gcgctacggc
    35341 gattgatcaa tagttcgagt gcatccattt gattaactcc tgtcgtgata tttattcaca
    35401 aaattaacac gagagtggat tttgttacag cacagtccgc aattcctgct gacaagtgcc
    35461 ggttgggtca ttacgataac cacatctatt gcgcctgtga caggtgtgac cttaagttgg
    35521 gagaatacat gcgaaccctt tggcgattta ttgccggatt ttttaaatgg acgtggcgtc
    35581 tgctgaattt cgtccgtgaa atggtactta acctgttctt tatttttctc gtgctggttg
    35641 gtgtggggat ctggatgcag gtcagtggtg gtgattcgaa agaaacggcc agtcgtggtg
    35701 cactgctgct ggacatttct ggtgtgatcg tcgataaacc cgacagttct cagcggttta
    35761 gtaaattaag ccgccaacta cttggtgcca gttccgatcg tctgcaggaa aactcactgt
    35821 ttgatatcgt caataccatt cgccaggcga aggatgaccg caatatcacc ggtatcgtga
    35881 tggatctgaa aaacttcgca ggcggcgacc aaccgtctat gcagtacatc ggcaaagccc
    35941 tgaaagagtt tcgtgacagc gggaaaccgg tttatgccgt tggcgagaac tacagccagg
    36001 ggcaatatta tctcgccagt ttcgccaata aaatttggct gtcaccgcaa ggcgtggtgg
    36061 atttgcacgg ttttgctacc aacggtctgt actacaaatc gttgctggat aagctgaaag
    36121 tttccaccca tgtgttccgc gtgggtacgt ataaatctgc cgttgaaccg tttattcgtg
    36181 atgacatgtc accggcagcc cgcgaagctg acagccgctg gattggtgag ctgtggcaaa
    36241 actatctgaa tactgttgcc gctaaccggc agatccctgc tcagcaggta ttccctggcg
    36301 cgcaagggtt gcttgagggt ttaaccaaaa ccggtggcga taccgcgaaa tatgcactgg
    36361 aaaacaagct ggtcgatgca ctggcatcga gtgcggaaat cgaaaaagca ctgaccaaag
    36421 agttcggctg gagtaaggct gataaaaatt atcgcgccat cagttattac gattacgcat
    36481 tgaaaacgcc ggcagatacc ggtgacagca tcggtgtcgt cttcgctaat ggcgcaatta
    36541 tggatggcga ggaaactcag gggaatgttg gcggtgatac cactgcggca caaatccgcg
    36601 acgctcgcct tgacccgaaa gtgaaagcga ttgtcctgcg tgttaatagc ccaggcggca
    36661 gcgttaccgc gtctgaagtg attcgcgctg aactggcagc agcccgggca gcgggtaagc
    36721 ctgtggttgt atcgatgggc ggcatggcgg catctggtgg ttactggatt tccacgccag
    36781 ctaattacat tgtggctaac cccagcaccc tgaccggttc tattggtatc ttcggcgtga
    36841 tcaccaccgt agaaaatagt ctggattcga ttggtgttca tactgatggt gtctcaactt
    36901 caccgctggc ggatgtttct atcaccaggg cactgccgcc ggaagcgcag cagatgatgc
    36961 agttaagcat tgagaatggc tataaacgct ttatcacgct ggttgctgat gcgcgtcatt
    37021 cgacgccgga gcagattgat aaaatcgccc agggccacgt ctggaccggt caggacgcaa
    37081 aagctaacgg gctggtcgat agtctcgggg atttcgatga tgcggtcgcc aaagcagcag
    37141 agctggcaaa agcgaaacag tggcatctgg aatactacgt tgatgaaccg accttcttcg
    37201 acaaagtgat ggacaacatg tctggttctg tccgggcaat gttgccagat gcgttccagg
    37261 ccatgttacc tgcaccgctg gcctcggtag cctctaccgt taaaagtgaa agcgacaagc
    37321 tggcggcgtt taacgaccca caaaaccgtt atgcgttttg cctgacctgc gccaacgtgc
    37381 gttaaatcct gcaaaaatgt ggccgatatg ctgtcggcca cactcctgaa tcggactgtc
    37441 tttcactccc tctctccact acattccctt gctcatttag catctgatat ggagtcatca
    37501 gtgaacaagg ccccctcttt aattgccgct atcgtcctcg gactggggat tagcgcctgt
    37561 ggttattttg ttggcgatgg tgttaaacat ttaaaaacca acaaccgtta tgtcaatgtg
    37621 cgcgggcttt ctgaaaaaga agttcgcgca gatacggcgg aattaacaat tgcgattaac
    37681 tttaagggta atgttcccgg cgaactgttc ccgaaactgg aagaagcgca gaaaaaaatt
    37741 gttgccgaac tcaatgctca ggggattaac gagaaagaga taattctggg ccaatggaca
    37801 agcaagcgaa cagattcctt ttatctgaaa gatgatccca caatgccacg ctataacgca
    37861 gacgggtcag tgacgataaa aacccataat gttgctgctg tcgaaaaagt cgtcgcgaaa
    37921 ttaaacgaac tgcaagtcgc tacggatggc gctatcgctg agagcaaagt cgcctatcgc
    37981 tttaatggta ttggcgcact gcgcgctgag atgattgctg ccgccaccaa agatgcacgc
    38041 aacgccgcac tacaatttgc aacggactca ggtagccagg tggggtcaat tagcgatgcc
    38101 tcacaaggcg tattccagat ctttgccagt ggcagtgatg aagatgatcc tacggcgatt
    38161 aataaaacgg tacgcgtagt cacgaccgta acctatgcgt tgcaggatta accacttttc
    38221 tccggcggga cactaatccc tccgtcctgc cggacttttc tcatatccac agagtatcca
    38281 ggtgcgctcg ctgcccctca cgtatatact tttgctcttt cgatatcatt catatcaata
    38341 tcatgcaaaa gaaatcaatt tacgttgcct acacgggcgg gaccatcggg atgcagcgtt
    38401 ccgagcaggg ttatataccg gtgtcaggtc atctacaacg ccaactggcg ctgatgccgg
    38461 aattccatcg cccggagatg ccagatttca ccattcatga atatacgccg ctgatggatt
    38521 cttcagatat gacgccggaa gactggcagc atattgctga agatattaaa gcgcactatg
    38581 acgactatga tggttttgtc attctgcacg gcaccgacac gatggcgtat accgcctctg
    38641 cgctgtcgtt catgctcgaa aatctcggta aaccggtcat tgtgacaggg tcacaaatcc
    38701 cgctggctga gttacgctct gacggacaaa ttaatctgct gaatgcgttg tacgttgcgg
    38761 cgaattatcc gatcaacgaa gtaacactct tttttaataa ccgactgtat cgcggcaacc
    38821 gcactaccaa agcccatgcc gatggttttg atgcgtttgc ctctccaaac cttcctccgt
    38881 tactggaagc aggtatccat attcgtcgtt tgaatacgcc acccgccccg cacggtgaag
    38941 gggaattgat cgtacatcca atcaccccac aaccaattgg cgtagtgacg atttatccag
    39001 ggatttctgc tgacgtcgtg cgcaattttc tgcgccaacc ggtgaaagca ttgattctgc
    39061 gctcctatgg cgtgggtaat gcgccacaaa acaaagcctt cctgcaggaa ttacaagaag
    39121 ccagcgatcg cggtattgtg gtggtcaacc tgacacaatg tatgtccggt aaagtgaata
    39181 tgggtggtta tgccaccggt aacgccctcg cccatgctgg cgttattggc ggtgcagata
    39241 tgactgtaga agccacgcta accaaactgc attacctgct tagccaggaa ctggatactg
    39301 aaaccattcg caaggccatg agccaaaacc tgcgcggcga actgacgccg gatgattaag
    39361 gagactgcaa tgccccctcg cgccctgtta ctggtcgatt tacaaaatga tttctgtgct
    39421 ggtggcgcgc tcgccgtgcc ggaaggtgac agtacggtgg atgtcgctaa ccgcctgatt
    39481 gactggtgcc agtcgcgcgg tgaagcggtt atcgccagtc aggactggca cccggcgaat
    39541 cacggcagtt ttgccagcca gcacggtgta gagccttata cgccaggcca actcgacggt
    39601 ttgccacaaa ccttctggcc agatcactgt gtgcagaaca gtgaaggcgc acaattacat
    39661 ccgttactga accaaaaagc gatcgcagcg gtgttccata aaggcgaaaa tcctttagtt
    39721 gacagttaca gtgccttttt tgataacggc cgtcggcaga aaacctctct cgatgactgg
    39781 ttacgcgatc atgaaatcga tgaattgatc gttatgggcc tggctactga ctattgcgtg
    39841 aagtttaccg tgctggacgc gttacagtta ggttataagg taaacgtgat taccgatggt
    39901 tgtcgtggcg tgaatatcca gccccaggac agtgcgcacg cgtttatgga gatgtcagca
    39961 gctggggcaa cgctatatac gctggcagac tgggaagaga cgcaggggta attttacgct
    40021 ggcctacaat tctgtactgg cattgtaggc caaataaaac acgtcagtgg cacatctggc
    40081 aattgatgcc atcaacgaaa gatttaattt acctcagaaa tctcttccaa cgacaccttc
    40141 cgcgtttcga tgccaaaaat ggagagaacc agcgcacaga gcaataacat gacaccaagt
    40201 accataaaca ccgtgatcga cccataatgt gttaataatg ccgcaacgcc atagggcgtg
    40261 aagactgcga cgatccgtcc gacggcatta acgaaacccg aaccgcgcag gcgtaaatgc
    40321 gttggccaaa gctccgggat ataaaccgcc gacgcgaagc aaacatacat gtataaaaag
    40381 aagatcatca ccaggccata gattaaaatc gcccactctg tagtctgaat tgaatagata
    40441 tagcctaaca ctgcaataat aataagtaag gtggagccaa ataaccgacg aggaaaatga
    40501 tcaataatta atgccgcaat aaatattcct accggagcgc caatcataat aacagcggtc
    40561 attaatattg atttatcgac atcaatgccg gagttaacaa atatggtcgg tatccatacg
    40621 gtgatggtat aaagcgaaat gttcattgca attaaaacag taatcgcgac taatgtacgt
    40681 cgtaacattt cacctttaaa caggagccag aaagtaccct tgattacttt aaccttgctg
    40741 ttgctctgat acgaagtaag cgggggtaaa cgaatactct tctctctttc aatttgctgc
    40801 tctacttcac gaagttggca ttctgcacct gcgatttgcc ctttccctgc cagccatcgt
    40861 ggcgactcaa taaagtattt acctgagaga aaccaggcta acagtatgcc aataccaccc
    40921 aacagaaaca ttatccgcca actaaaaaaa gcgataacca ccacacctat cgccgcagac
    40981 agcatgggcg accagttacc aacaaatgag agccgcgcgg accattttcc acgcaccgtc
    41041 gcggggataa actccgtaaa tgaggcatag ccaaccataa tcagcgcccc cattcctgtt
    41101 cccatcagga agcgaaagaa gataagccag tacatatcag ggacaaaagc ggcccctgtt
    41161 gcagcaatac cgacgatgag aagatttatg cgaaacgccc tgcgccgccc aaagtaatca
    41221 ccgatgaacc caccggtaag agagccgatg aaataaccaa acattaatgc cgaggtaaag
    41281 gcggcattga ggaaattatt tgaccatcca ttgcttacca gctttgctaa gacgacatta
    41341 ccggagtaac tcaaaaaccc t
//
LOCUS       DAZFTN010000032        40849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFTN010000032 DAZFTN010000000
VERSION     DAZFTN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..760
                     /gene="espX2"
                     /locus_tag="ACHKV2_001850"
     CDS             <1..760
                     /gene="espX2"
                     /locus_tag="ACHKV2_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO6939744.1"
                     /translation="RVMRLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDP
                     ETICMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVLECNNQ
                     PTELNSSFSSLMNDSDQWQQHTLKDKHYANLLTMLDLNDASESDKSKIFFCLSAVFAN
                     ISHSNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEE
                     ANQLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1044..3194
                     /gene="dinG"
                     /locus_tag="ACHKV2_001851"
     CDS             1044..3194
                     /gene="dinG"
                     /locus_tag="ACHKV2_001851"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO6939745.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3222..4184
                     /gene="ybiB"
                     /locus_tag="ACHKV2_001852"
     CDS             3222..4184
                     /gene="ybiB"
                     /locus_tag="ACHKV2_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO6939746.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4325..5410
                     /gene="hcxB"
                     /locus_tag="ACHKV2_001853"
     CDS             4325..5410
                     /gene="hcxB"
                     /locus_tag="ACHKV2_001853"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO6939747.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5477..5536
                     /gene="ybiE"
                     /locus_tag="ACHKV2_001854"
     CDS             5477..5536
                     /gene="ybiE"
                     /locus_tag="ACHKV2_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO6939748.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5639..5899)
                     /gene="ybiJ"
                     /locus_tag="ACHKV2_001855"
     CDS             complement(5639..5899)
                     /gene="ybiJ"
                     /locus_tag="ACHKV2_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO6939749.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6164..6430)
                     /gene="ybiI"
                     /locus_tag="ACHKV2_001856"
     CDS             complement(6164..6430)
                     /gene="ybiI"
                     /locus_tag="ACHKV2_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO6939750.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6506..7192)
                     /gene="ybiX"
                     /locus_tag="ACHKV2_001857"
     CDS             complement(6506..7192)
                     /gene="ybiX"
                     /locus_tag="ACHKV2_001857"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO6939751.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7234..9516)
                     /gene="fiu"
                     /locus_tag="ACHKV2_001858"
     CDS             complement(7234..9516)
                     /gene="fiu"
                     /locus_tag="ACHKV2_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO6939752.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADVKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9781..10041)
                     /gene="mcbA"
                     /locus_tag="ACHKV2_001859"
     CDS             complement(9781..10041)
                     /gene="mcbA"
                     /locus_tag="ACHKV2_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO6939753.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10317..11243
                     /gene="rlmF"
                     /locus_tag="ACHKV2_001860"
     CDS             10317..11243
                     /gene="rlmF"
                     /locus_tag="ACHKV2_001860"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO6939754.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11240..13465)
                     /gene="ybiO"
                     /locus_tag="ACHKV2_001861"
     CDS             complement(11240..13465)
                     /gene="ybiO"
                     /locus_tag="ACHKV2_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO6939755.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13582..14304)
                     /gene="glnQ"
                     /locus_tag="ACHKV2_001862"
     CDS             complement(13582..14304)
                     /gene="glnQ"
                     /locus_tag="ACHKV2_001862"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO6939756.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14301..14960)
                     /gene="glnP"
                     /locus_tag="ACHKV2_001863"
     CDS             complement(14301..14960)
                     /gene="glnP"
                     /locus_tag="ACHKV2_001863"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO6939757.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15098..15844)
                     /gene="glnH"
                     /locus_tag="ACHKV2_001864"
     CDS             complement(15098..15844)
                     /gene="glnH"
                     /locus_tag="ACHKV2_001864"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO6939758.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16248..16751)
                     /gene="dps"
                     /locus_tag="ACHKV2_001865"
                     /gene_synonym="pexB"
     CDS             complement(16248..16751)
                     /gene="dps"
                     /locus_tag="ACHKV2_001865"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO6939759.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17050..17937)
                     /gene="rhtA"
                     /locus_tag="ACHKV2_001866"
     CDS             complement(17050..17937)
                     /gene="rhtA"
                     /locus_tag="ACHKV2_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO6939760.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18172..18237
                     /locus_tag="ACHKV2_001867"
     CDS             18172..18237
                     /locus_tag="ACHKV2_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939761.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18290..18805
                     /gene="ompX"
                     /locus_tag="ACHKV2_001868"
     CDS             18290..18805
                     /gene="ompX"
                     /locus_tag="ACHKV2_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO6939762.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18854..20437)
                     /gene="opgE"
                     /locus_tag="ACHKV2_001869"
     CDS             complement(18854..20437)
                     /gene="opgE"
                     /locus_tag="ACHKV2_001869"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6939763.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20709..20837)
                     /gene="mntS"
                     /locus_tag="ACHKV2_001870"
     CDS             complement(20709..20837)
                     /gene="mntS"
                     /locus_tag="ACHKV2_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO6939764.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21023..21490
                     /gene="mntR"
                     /locus_tag="ACHKV2_001871"
     CDS             21023..21490
                     /gene="mntR"
                     /locus_tag="ACHKV2_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO6939765.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21487..22605
                     /gene="ybiR"
                     /locus_tag="ACHKV2_001872"
     CDS             21487..22605
                     /gene="ybiR"
                     /locus_tag="ACHKV2_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO6939766.1"
                     /translation="MSLLFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22663..23583)
                     /gene="ldtB"
                     /locus_tag="ACHKV2_001873"
     CDS             complement(22663..23583)
                     /gene="ldtB"
                     /locus_tag="ACHKV2_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6939767.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23802..25394
                     /gene="ybiT"
                     /locus_tag="ACHKV2_001874"
     CDS             23802..25394
                     /gene="ybiT"
                     /locus_tag="ACHKV2_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO6939768.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25562..26827)
                     /gene="ybiU"
                     /locus_tag="ACHKV2_001875"
     CDS             complement(25562..26827)
                     /gene="ybiU"
                     /locus_tag="ACHKV2_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO6939769.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(26979..27794)
                     /gene="ybiV"
                     /locus_tag="ACHKV2_001876"
     CDS             complement(26979..27794)
                     /gene="ybiV"
                     /locus_tag="ACHKV2_001876"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO6939770.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(27940..30371)
                     /gene="ybiW"
                     /locus_tag="ACHKV2_001877"
                     /pseudo
     CDS             complement(27940..30371)
                     /gene="ybiW"
                     /locus_tag="ACHKV2_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(30377..31276)
                     /gene="ybiY"
                     /locus_tag="ACHKV2_001878"
     CDS             complement(30377..31276)
                     /gene="ybiY"
                     /locus_tag="ACHKV2_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO6939771.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31407..32069
                     /gene="fsa"
                     /locus_tag="ACHKV2_001879"
     CDS             31407..32069
                     /gene="fsa"
                     /locus_tag="ACHKV2_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6939772.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32248..32997)
                     /gene="moeB"
                     /locus_tag="ACHKV2_001880"
     CDS             complement(32248..32997)
                     /gene="moeB"
                     /locus_tag="ACHKV2_001880"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO6939773.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(32997..34232)
                     /gene="moeA"
                     /locus_tag="ACHKV2_001881"
     CDS             complement(32997..34232)
                     /gene="moeA"
                     /locus_tag="ACHKV2_001881"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO6939774.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            34436..35401
                     /gene="iaaA"
                     /locus_tag="ACHKV2_001882"
                     /pseudo
     CDS             34436..35401
                     /gene="iaaA"
                     /locus_tag="ACHKV2_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            35388..37259
                     /gene="gsiA"
                     /locus_tag="ACHKV2_001883"
     CDS             35388..37259
                     /gene="gsiA"
                     /locus_tag="ACHKV2_001883"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO6939775.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37279..38817
                     /gene="gsiB"
                     /locus_tag="ACHKV2_001884"
     CDS             37279..38817
                     /gene="gsiB"
                     /locus_tag="ACHKV2_001884"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO6939776.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38835..39755
                     /gene="gsiC"
                     /locus_tag="ACHKV2_001885"
     CDS             38835..39755
                     /gene="gsiC"
                     /locus_tag="ACHKV2_001885"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO6939777.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39758..40669
                     /gene="gsiD"
                     /locus_tag="ACHKV2_001886"
     CDS             39758..40669
                     /gene="gsiD"
                     /locus_tag="ACHKV2_001886"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO6939778.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         9736 a        10143 c        11014 g         9956 t
ORIGIN      
        1 gcgagtaatg cgcttatcgc accaggcgtt aatctcaatg cttgagccag aggttaagaa
       61 aaagactatc tggaataatt actctattta tccatctttg caggataccc atgaagttgt
      121 cagagatgat ccagaaacga tttgtatgcg tgcatttcct ttatttgcaa aaggatggga
      181 atatgcgcag aaaaataaga aacatcaatt gatcctcaat gctcttggat tcaaaggtta
      241 tattcgtgat atatttatgt cagcaataat gcgaaaaaca gattttgtgc tcgaatgcaa
      301 taatcaaccg acagaactta attcatcatt ttcctctttg atgaacgatt cagatcaatg
      361 gcaacagcat actctgaaag ataaacatta cgctaatcta ttaacgatgc tggatctcaa
      421 tgatgccagt gaaagtgata agtctaaaat atttttctgc ctgtctgccg tatttgcaaa
      481 tatttctcac agtaatgtct ttaacggtat tcctgatgcg tcaaaaactt taaaacgtta
      541 tgcatttgct ttattggcga aagctcattc tttagatgaa agtatgataa gcaatcaaac
      601 ttttaataca tacaaaacgg tattgcttga tttcaataat ctgtctaatg aagaggccaa
      661 ccagttgcgc ataagttcgt tatatcgtga tatggtccgc tatgcacaat atcgtttttc
      721 taaagtttta tccgagtgga cgccagacgc atggctgtaa agatcgcagc agaataatgg
      781 cattgatggc cctggctcag gccagggtcg gcaataccgg ttcctgcgaa gcgatgacgg
      841 cggcaattgt aaccacgaaa tgcggtatca cttgctgttt tctgccctga agactggcgc
      901 tcactgtgcc gtcgaatttt caatcaaata tattgcgaga gggcgtgttt gcgagccgct
      961 ttccagaaac agaaaaacca ttacccctga aaaccgaaaa atgccacaat attggctgtt
     1021 tatacagtat ttcaggtttt ctcatggcat taaccgccgc gcttaaagcg caaattgccg
     1081 cctggtataa ggcgcttcag gaacagatcc ccgactttat tccccgtgcg ccgcagcggc
     1141 agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga agggcggcat ctggcgattg
     1201 aagcccccac tggcgttggg aaaacgctct cctatttgat tcccggcatc gccattgccc
     1261 gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt ggcattgcag gatcagatct
     1321 acagcaaaga tttgccactg ctgaaaaaga tcattcccga tcttaaattc actgccgctt
     1381 ttgggcgtgg gcgctacgtt tgcccgcgta atctgacggc gcttgccagc actgagccca
     1441 cgcaacagga tctgctggcg tttcttgacg acgaactgac gccaaataat caggaagagc
     1501 aaaaacgttg tgcgaagctg aagggcgatc tcgacactta taaatgggat ggtctgcgcg
     1561 atcatacgga tatcgctata gatgacgatc tctggcgtcg tttaagtacc gacaaagcca
     1621 gctgcctcaa ccgcaactgt tactactatc gcgaatgccc gttttttgtc gctcgtcggg
     1681 aaattcagga agcggaagtg gtggtggcaa accatgcgct ggtgatggcg gcgatggaaa
     1741 gcgaagccgt attgcctgac ccgaaaaatt tactgctggt gctggacgaa ggccatcacc
     1801 tgccagatgt ggcgcgggat gcgctggaga tgagtgccga aatcaccgcg ccatggtatc
     1861 ggctacagct ggacttgttc acgaaactgg tcgctacctg catggagcag tttcgcccga
     1921 agaccatccc gccgttggcg atccctgaac gtttgaatgc gcattgtgaa gagctgtatg
     1981 agcttatcgc ctcattaaac aacattctca atctctacat gcctgccggg caggaagcag
     2041 aacaccgttt tgcgatgggc gaactgcctg atgaagtgct ggagatctgc cagcggctgg
     2101 caaaactcac cgagatgctg cgtggtctgg cggagttatt tcttaacgat ttaagtgaga
     2161 aaaccggcag ccatgacatt gtacgtctgc atcggttgat tttgcagatg aaccgcgcgt
     2221 tggggatgtt cgaggcgcaa agcaaactct ggcggctggc ttcgctggcg caatcttccg
     2281 gtgcaccggt gaccaaatgg gcgacgcggg aagagcgcga agggcagcta cacctctggt
     2341 ttcactgcgt gggaatacgt gtcagcgatc agctggaaag gctgctgtgg cgcagtattc
     2401 cgcacattat tgtcacctcc gcaaccttgc gttcgctgaa cagtttttcg cgtttgcagg
     2461 agatgagcgg gctgaaagag aaagcgggcg accgttttgt ggcgctggat tcccccttta
     2521 accactgcga acagggcaaa attgttattc cccggatgcg cgttgagcct tccatcgaca
     2581 acgaagaaca gcatattgct gaaatggcgg cctttttccg tgagcaggtg gagagcaaaa
     2641 aacatctcgg tatgttggtg ctgtttgcca gcgggcgtgc gatgcagcgc tttctcgact
     2701 atgtgacgga tttacgtctg atgttgctgg tgcagggcga tcagccgcgt taccgtttag
     2761 ttgaactgca ccgcaaacgc gtcgccaacg gtgagcgtag tgtgctggtg ggcttacagt
     2821 catttgccga agggcttgat ttgaaaggtg atatgctcag ccaggtgcat atccacaaaa
     2881 tcgcttttcc gcctatcgac agcccggtgg tgatcactga aggggaatgg ctgaaaagcc
     2941 tcaaccgcta tccatttgag gtgcaaagcc tgccgagcgc ctcgtttaac ctgattcagc
     3001 aggttgggcg actgattcga agtcacggtt gctggggcga agtggtgatt tacgataaac
     3061 gcttgctgac caaaaattat ggcaagcgac tactggatgc attaccggta tttccgatag
     3121 agcaaccgga agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa tccccacgcc
     3181 gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta aatggactat cgcaaaatca
     3241 ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat actgcgcgcg
     3301 gtctgtatgc ccatatgctc aatggtgaag tccctgacct tgagttgggc ggtgtattga
     3361 ttgcgctgcg tatcaaaggg gaaggggaag cagagatgct cggcttttac gaagccatgc
     3421 aaaatcacac catcaagctg acgccgcctg caggcaagcc gatgccgatt gtcatcccca
     3481 gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt gttggcgatt ctcctgcaca
     3541 aactcggttt tcctgtggtg gttcacgggg ttagcgaaga tccaactcgc gtgctgaccg
     3601 aaaccatttt cgaactgatg ggtattacgc caacgttgca cggcgggcag gcgcaggcga
     3661 agctcgacga acatcaaccg gtgtttatgc ctgtcggcgc gttttgcccg ccgctggaaa
     3721 aacaactggc gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc ctggcgaaac
     3781 tggcgacgcc atttgccgaa ggtgaggcac tgcgtctttc cagcgtttcg catccggaat
     3841 acatcgggcg agtcgcgaag ttctttagcg atatcggcgg gcgggcgtta ctaatgcatg
     3901 gtactgaagg cgaagtgtat gctaatccgc agcgctgccc acagatcaat ctcattgatc
     3961 gtgaagagat gcgggtgctg tatgaaaaac aggaccctgc tggaagcgag ttactgccac
     4021 aagcaaaaga tccggaaacc acggcgcagt ggattcagcg ttgccttgct ggccgcgaac
     4081 cgattcccga atcgctgaaa atccagatgg cttgctgcct ggtggctacg ggtgaagcgg
     4141 caactatcag cgatggcctg gcgcgcgtta atcaggcatt ttaattcttt tctcagccgg
     4201 atgagccatg tcatccggct tttaccccac cgcaatatga aattcctgca tctttattga
     4261 ccttcccgcg cccggcgtgc agcataaaaa aacaacaaac acataacata aacaggagtt
     4321 aaccatggaa agtggtcatc gctttgatgc tcagacgctg cacagcttta ttcaggctgt
     4381 atttcgtcag atgggtagcg aggaacaaga agcgaaatta gttgccgatc atttaatcgc
     4441 ggcaaacctg gcagggcatg attcacatgg tattggcatg atcccaagct atgtacgctc
     4501 ctggagtcag gggcacctgc aaattaacca tcatgccaaa atcgttaaag aggcgggggc
     4561 ggcggtcacg ctcgatggcg atcgcgcatt tggtcaggtc gcggcacatg aagcgatggc
     4621 gctggggatt gagaaagcgc atcagcacgg tattgctgcc gtggcgctac ataactcgca
     4681 tcatatcggc cgtatcggtt actgggcgga gcagtgtgca gcggcggggt ttgtctctat
     4741 ccactttgtt agcgtggtcg gtattccaat ggtcgcaccg ttccacggtc gcgacagccg
     4801 ctttggcacg aatccgttct gtgtggtttt cccacgtaaa gatgattttc cgctgttgct
     4861 tgattacgcc accagcgcca ttgcatttgg caaaacccgc gtcgcctggc ataaaggcgt
     4921 ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg ccgacgacca atccggcggt
     4981 aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc gaacataaag gctacgccct
     5041 ggcggcaatg tgtgaaattc ttggcggggc gctttctggc ggtaaaacga cgcatcagga
     5101 aacgttacaa accagtcccg atgccattct taactgcatg accactatca tcatcaaccc
     5161 ggaactgttc ggcgcgccgg attgtagcgc gcagaccgaa gcctttgccg agtgggtgaa
     5221 agcctcgccg catgatgacg ataagccgat tttgctcccg ggcgagtggg aagtgaacac
     5281 gcgtcgcgaa cggcaggagc aggggattcc attggatgcg ggaagctggc aggccatttg
     5341 tgatgcggcg cggcagattg gtatgccgga agagacgttg caggctttct gtcagcagtt
     5401 agccagctaa aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa cataggatca
     5461 atgagggtta gagcatatgc gtctgtcggc aaacagacag ggaaatactt gtgcaggacg
     5521 tagcgtaaac gcctgatccg tccaacggtt cggattttgt aggcctgata agacgcggta
     5581 gcgtcgcatc aggcatctgt tgccggatgc ggcgtaaacg ccttatccgt cctacgaatt
     5641 acttataaat taccgcagta ccgcttaatt tattgttgtt ggtggcggaa gtgatgctat
     5701 atccactggc accggctgcc gccgctttct cagccagttt cgcttccagg gcatcgaggg
     5761 tggatgcgcc atcggcagaa accacgccga ttttattcat gttctgtgcc tgggatgccg
     5821 ttaccggttc cgcggcaaac acgccaaatg acagagctga aagagccata gcagcaacaa
     5881 cagtattgat agttttcata attaatctct cgcaggtgat ttttgtataa gtgacgttgt
     5941 ttcgtcgatg tgatgagtat cacgtttttt tacgagagat aaaatcgaag agaattgacc
     6001 gccttgttca aataaattga ttgatatcta acatactgat aaactattta tttgttgtac
     6061 ttatggactt gttttatgca gcgtggcagt cacgacagcg ttaaaggcac ggaatgccac
     6121 gcaaaataca aattatactc gctatacatc tcgccagttg tagttaacgt aactggcttt
     6181 ctttcgaacc tctgcgatta tatcctgtat aagctggttt ttgtaaatct ttctcctgct
     6241 gacaatgaat acataagcgc acgccaggaa tggcttcccg acgggcttgc gggatggggg
     6301 caccgcactc ttcacattca tacaggcttt cgccacgcgg aatttcaccc cgagcgcggg
     6361 caatcgcatc ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg ttagcccaac
     6421 cggatgccat atcgtcctcc ccatatatca atgcttgtat agttaagtgt agctaatata
     6481 gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc agattatgat
     6541 aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc agcgactgaa
     6601 tattctgaat atttttgtcc agttcaaaca gcatggcgcg ctttttatca tcgcggatca
     6661 tcgactggat ccatataaat gatgccactc gtacgccgcg ggttacgggt gtcacgcaat
     6721 gcaggctgct ggagggatac aacaccagat cacctgccgg gagttttacc cgatgttgtc
     6781 cgaaggtgtc attaacgacc agttcgccgc cgtcgtagct ttgtggatcg cttaaaaaca
     6841 gcgtggcaga aaggtcagtg cgcatccagc cgttttgtgg atggctgcgt actgcgccat
     6901 ccacatgaaa accataggtt tcattgttct gatagcgatt aaacagcggc gtggaaaggg
     6961 tacgcggcaa ggccgcggca aagaacaaag catgttggtt aaccgcgttc agcacctcat
     7021 tttgcagggc ggcgtataac gtgctgcggg tgtctacctg ttgattgttc ttaacttgcg
     7081 caccttgtgc gccggtggtg acgcgtccat ccacccattc ggcttgttcc agttgttcgc
     7141 gaaaacgggc gacgtcctgt ggcgataaca cgccgggaat gtggtacatc atacaagttc
     7201 tccaaaaagt ggggcctgcg ccccacatct gaatcagaaa tgcatattgg ctgtgagcaa
     7261 gaaggttctt ggctcgcccg ggtgataacg gtagccgctc ttattgattg aggcgacgta
     7321 atcggtatca aacaggttgt agacgtttag ctggaagtcg agattgcgat taaccctata
     7381 ccccagtttg acatcggcga cccagtaacc ttcggtaaac gctggcgttc ccaccgcgcc
     7441 gtctgaacct ttatgcatac tgccgatata gcgtgcgccc gcgccaacag agatatcatc
     7501 ggttgcctga tattggctcc ataaggtgaa ggcgtgctcc ggggtatacg gcagcgatga
     7561 ggaaccatcc tgggcaacat ctttgccgtt tttgatggtt gctttttgct gggtatagcc
     7621 gccaatcacc tgccacgcgg gagtgatatt ccctgccacg gatatctcat agccttcgac
     7681 gcgtttctta ccgtattgcg agtaagtccc gtcatcattt tgctcaactt cattttcgat
     7741 atcagtacgg aacagcgcgg cggtgagcaa caggcgttta tccagaacct gccatttggt
     7801 gccaatctcg ctggtgttgg ctttttgcgg tttaaaatcg gtgcggttgg cactgttacc
     7861 gctgccagac tgcgcaaggg cgaagttgtt gccgcccgga ggctgctggg aaacggcata
     7921 gttaatatag acattgccgt tttccgtcag gtgatacagc gccccggctt tccagttcac
     7981 cagattgccc gacttggcgg tgtcgacggt ggtgaccgga gaaccttttg ccacaccagc
     8041 tgggcaggtg atggcaccgc gtccgctgcc gccgcaggcg gtggcactgt catattcagt
     8101 atgataatta tccagacgga tcccgccgtt cagctcaaaa tcacgggtga tttgcagcgt
     8161 atcgaatgcg taaattgcga aggtatccgt ctgaccattg gcgtttgcgc cgttgcgcgt
     8221 caggccgccg ggatgaatgc tgctgtcagg atgataaata tttaccgcag gtaacgtcac
     8281 cggattaacg ccgtagttag tctgcgtttc acgggtaaat tccacgccgg tactgacatc
     8341 atgaccgata gaagcggtat agaacgtcga ggtcaggttg gtctggttgg tgagaatttt
     8401 attactcaca tctttggtat tcgccgtgcg tgaccacgtc cagctattca catcgctggt
     8461 gggctgggta atattcgacg ccccacccat aatcgccgtc atcaggtaat cctgcttcac
     8521 gcgcgaccaa cgggtagtgt tgcgaatggt ggtgttatcg ttgatatcgt gctcaaaacg
     8581 catggtggcg gtgtcggtgg tcgaatcgtc gtaatcggaa tccgtgccgt aaaagttatg
     8641 cgtatcaact tttccggaat gattcagggt cgccgttcct gcagatgggg cagaatagcc
     8701 cggcaaaccg atggttggaa tgccgccgtc tggcgtgttg tgctgggtga catgcagata
     8761 attaagatac aaacgattcg ctgtaccaag gccaaaagcg atagaagggg cgacgccgta
     8821 acgctcattt ttgactttgt cgcgtccggc atcgtgcgtt ttttcgccca tcacattcag
     8881 gcgcaccgca gtggtatcac caatgacctg attgacgtcc agcgtgccgc ggcggaacca
     8941 ggcgctgcca atactggcgg aggcgtcaat gccggaatca ttgcgcggct gcttgctgat
     9001 catattgatc gagcccgtcg gcgcgctgcg cccgtagtcg gtgccggaag gccctttaat
     9061 cacttcgacc tgctcagtat tgaaggtgtc gcgcgagacg ctgccgatat cgcgaatgcc
     9121 atcaatataa atactgttag aggtatcagc accgcgcata taaatggcgt cgccagtggt
     9181 ggagttaccg ttctcacccg caaaaaacgc acccacgccg gggacgtttt tgagcgcatc
     9241 ggtaaggttg gttgcgccct gatctttaat cacttgttca gaaatcaccg tcatcgtgcg
     9301 ggtagtatcc gctaccggac gcgagaattt cggatcggca gattgttgtg gcgcataaag
     9361 cgaaggcgtt gatgcttcga caaccagcgt gtcatccgcg ttagtttgcc cttcggcggc
     9421 gagtgcctga gccacaggcg tgatgccaat acaaagaccg gcaaagaacg tgagcgaatg
     9481 aaattgtctg gcagggaaat tgcgattgtt ttccattttg caggtgactt tttcttatat
     9541 gttgaaattt tcactgccac gagaaagcct ctctgcggat gcgatgccac tgttaatctc
     9601 gtgatagtgt tcgtgcaagt gataataatt ttgataagca ttatcacttg gcgcgatttt
     9661 ctatcaccca ttgcgcgaaa aagaaataca tttatttaca ttgcgttcat atttgatgtt
     9721 ttttgattac aaaacaggcg aaaactgcat ccagaggcga atgcagttac gttggtgggg
     9781 ttatttgtag atggttgcag taccgaacat ctggtcattt ccgcctgcgg aattaatgac
     9841 ataacctttc gcgccttgct cgcgcgcttt ttctgccagt ttatcctcca gatcgcttag
     9901 gtttgatgcg ccagttgccg aaacggtacc ggcgggccgt agctgaccgc tgtccaggtt
     9961 actcatcggt tgcgcggcat aagccgtgag agagattccg ctcaggacgg tggcaatcag
    10021 tagtgtgagg cactttttca taatgacatc ctcataaata caacgggtag tgcctgataa
    10081 gtgtaggtcg cctgcgcgat gcaaatgacg caaaaaattg acagagatgt gcttattttt
    10141 tgaacgatat atttttacaa aataagacaa atcaatgaca tgcataatgc tgatttttat
    10201 gatgaattgc cataaaccat agccatgtta aggttttggg gaatgataaa cgcagccaga
    10261 tatttaccct gtcgcgttat gcgggtatct tacgccgctg cttaaaggag aatgctatgt
    10321 ccgcccagaa accggggttg catccgcgca accgtcatca cagccgctac gatctcgcca
    10381 cgctttgtca ggtcaatcct gaactcaagc aattcctcac gcttacaccc gccggggagc
    10441 aaagcgtaga ctttgccaat ccgctggcgg tgaaggcgct caataaggcg ttgctggccc
    10501 atttttacgc cgtagcgaac tgggatatcc cggacggttt tctctgcccg ccagtaccgg
    10561 gccgggcgga ttatattcat caccttgccg atttactggc tgaagcaagc ggaacaattc
    10621 cagctaatgc cagcattctt gatatcggcg ttggtgcgaa ctgtatttat ccgctgattg
    10681 gcgtacatga atatggctgg cgttttaccg gtagtgaaac cagcagccag gcgttaagca
    10741 gcgcgcaggc gatcatcagt gctaatccgg ggcttaatcg cgccattcgt ctgcgtcggc
    10801 aaaaagagag tggggcgatt tttaacggca tcatccataa aaacgagcaa tacgacgcga
    10861 ccttgtgtaa cccgccattc cacgattccg ccgctgcggc gcgggcaggt agtgagtgta
    10921 aacgccgtaa cctggggctg aacaaagacg atgcactgaa ctttggcggc cagcaacagg
    10981 agttgtggtg tgaaggcggt gaggtcacct ttatcaaaaa gatgattgaa gagagcaaag
    11041 gcttcgcgaa gcaggtgatg tggtttactt cactggtatc tcgtggtgaa aacttaccgc
    11101 cgttgtatcg tgccctgacg gacgtgggcg cggtgaaggt ggttaaaaaa gagatggccc
    11161 aggggcaaaa gcagagtcgc tttattgcct ggacctttat gaacgacgag cagcgccgcc
    11221 gttttgtcaa tcgccagcgt taaagcgttg gctcccccgg cggtaacggt tcagccgggg
    11281 tcgcgcccgg agcaggcagc acctgataag tctgcaccgg cgcgcgaacg cccgccaggt
    11341 cgaaatgttt tttcacctgg ctgtcgaggg caaagcgtac cgtccactgt ttgagtggca
    11401 gcgtggtgaa cgaaacacgc agtgtaaacg cggtattgct taagccaaca atcccggcaa
    11461 agttcggttc accaataatc agcccacgaa tttcttcgtt ttccattaat tccgctaccg
    11521 catctttcag tgcctgattg gctttatcag catcttcatg gcgatcaaca tcgtagtttg
    11581 ccactaccga accaatgccg cggacaaagt tggcaaaggt ggtaatcgaa gaccacggaa
    11641 tgatgtggta cgccccggta tcctggcgta cgcccacgga gcgaatcgac atccgttcca
    11701 cagtgccggt caacggccca atagtcacca aatctccagt gttcatgccg ttttcaaact
    11761 gaataaatac cccggtgata atatctttca ccagcgtttg cgaaccaaac gagatagcca
    11821 gacctaatgc cccggcacct gccagcaatg gcgcgatatt gacgccgatt tccgacaaca
    11881 caatcatgat ggtgatggta ctgataatca ccgccagcgc gttacgaaac agcgtaagca
    11941 gggtacgcgt gcgggcgctg ggtagcgggc ggccatgaat atccgaagcc agccggtttt
    12001 cgatcaaact ggcgagcacc gtccagccaa ccgccgagaa gaaaagaatg agtgcgatac
    12061 ggatcaggat atctacggtt ttctgccccg cgccgttttg cagccagttc cagaaatcga
    12121 acaatcccca tgcgctcaac agcagcatta ccgccacgca gacggtcaga atacgcgccg
    12181 ttttcagcgc cgccgaaagc cagccattca gccgtttttg caactccgga tagttacgct
    12241 gagtatgtgg cgagagagtg atggtttttg ccagccagcg ggaaaacata ccggaaacaa
    12301 atgctgcgat accaataatc gccaggctgc gcactgttgc acccatcata aatttcaggc
    12361 tgttgcccgg atcgaacaac gaaaagaaaa acagcacgat aaaataggcg cttgccagcc
    12421 agtgccacac cagcgcaaag gcgcggataa acaggctgaa aaaggccagc gaatgctccg
    12481 caaagttgag caaatgctgg gtaatctctt ttttattacg aaagatcagg tacaacgccc
    12541 agacggtcat gcacagcata atgatgacgt tcgccagcgc acctatctgt acattcacct
    12601 gattagagat aatcggcacg gccacaatca ggccataacc tatcaaactg cctaaccagc
    12661 tcaggcgacg actccagtaa cgggcgctct cgtcctgaat cgtgaacggg cgcagttccg
    12721 ccacgtttgg gcaaaaaatc aggcgtagta cggctttgaa aaattcaatc agggcaaagg
    12781 cgttgagaaa caaactttgt tggaaagcga tggtgcgact gcctgcattc aggttgtcgc
    12841 ttaatatctg gccgacaaat aatgtcagtg ccagtaacag caggtcgata ataaacgccc
    12901 cgataatcat cgccggaagc tgcagccagt tactgcgctc acgatttttt tgccgcgccc
    12961 actgacccat tttgcgatac agcggcagtg cgcacaggcg aatcagccag taaaaaccaa
    13021 acactaaaac cgctaacatt gaaaaatggg tcagcgcatt gctgaaggtt tgtggattaa
    13081 acggcttatg cggggagccg gtgatattgc gataaagctg cccgaagcgg gcggaaaggg
    13141 cttcaccata atggcggctg aattcggtga ccttttgcag cacggtttgc tcttcaacca
    13201 gcgtcggcgg cacgatcttc gggaccggtt cagcaggtgg cgtcgcggcg acggtgcgca
    13261 actggtcgat caactcttta cgcgaggtgt cattatccag cacatccgcc agtgcgccat
    13321 aagccgcttt tttttgttcg atatccggtt ccggggccgg ttcagtcgtt gagtccgttg
    13381 tcgttgtggt tgtaacgccg ggtatggata ccgcgtgggc aggtgcgccc agcaggcaga
    13441 agaggatgaa caggatccac cgcatgactc ctccagtgag aaaacagagc aaaaagataa
    13501 gtatagatgc tgggggaggg gtgattttaa atgagaggaa tctggtgtac ctccctttcg
    13561 ggtgaaaggg aggcagagaa gttaagagac gtgctgtaaa aattcctgca agcgctggct
    13621 cggcggattt ttaatcaaca cttgtggatc gccatcttcc gcaatccggc ctttgtcgat
    13681 aaagatcagg cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca cgatcaccat
    13741 cgtcatccct tcttcagcca gatcttgcat aaccttcagc acttcatggc gcagttccgg
    13801 gtcaagagcg gaagtcggtt catcaaacag catcattttc ggcttcaccg ccagcgcgcg
    13861 ggcaatcgcc acacgctgct gttgaccacc agaaagttcg gaagggtagt gatgtgcacg
    13921 ttctgccaga ccgactttcg ccagcagctc acgtgccagt ttttccgcct cttctttgtt
    13981 cgcgccacgc acgcgtagcg ggccaaacat gacgttttcc agcgccgtca gatgcgggaa
    14041 gaggtaaaac tgctggaaca ccatacctgc ttcctggcga atcaggcgct cgtcaacttt
    14101 cggatcgtta accttcaggc catcgacaat cagatcgccg gaggtgattt cttccagttt
    14161 gttgatgcag cgcagcaggg tcgatttacc ggaaccggac ggcccgataa tcaccacgac
    14221 ttcgccctgg gcaatgttca aatcgatatt gtgcagcacc tgggttgggc caaagtgctt
    14281 ggagacgttt ttaaattcaa tcacaggatt ttcatccttc tttccagacg acgcagaata
    14341 aagctcagca ccagggtaat aatcagatag aacaccgcca cggcgctcca gatctcaagg
    14401 gcgcggaagt taccggcaat aatttcttgc ccctgacggg tcagttccgc cacgccgatc
    14461 acaataaaca gcgaggtgtc tttaatgctg atgatccact ggttacccag cggcggcagc
    14521 atacgacgca gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg tgaaagaccg
    14581 agcgccagtc ctgcttcacg aaaacctttg tggatagaca gcaccgcacc acgcgtgatt
    14641 tccgcaatgt acgcgccgga gttgatcatg atggtgacca ccgccgcagt aaatgggtcg
    14701 atgcgtaagt cattaaacgc catcggcagg gcaaaataga taaacatcac ctggacgacg
    14761 ataggtgtgc cgcggatcac ttcaataaaa accagcgcga cgtggttggc tatccaacca
    14821 ccgaaggtgc gtgcaaaacc tgccagcaat ccgatcacca ggccgccagc cagaccgagg
    14881 accgatatcc acagggtcat tttggcacct tcaatcagaa gcggaatggc aggccagata
    14941 gcactccagt caaactgcat atgttgttcc tgttaccgtg gtgtaaataa tcaaattcag
    15001 ggtaattaat ggccccggag cgggtttgcg ccctcaccct aatcctctcc ccagagggga
    15061 gagggaactg ccagtgcgct ttacaggtgt agcgttatta tttcggttca gtaccgaacc
    15121 attttttgta gatttcgttg taagttccgt tctcgcgcag ggttttcaac gcgccgttga
    15181 ctttgtcacg cagctcgtcg ctacctttcg ggaacgcaat accgtattgc tgcgcttcca
    15241 gagagtcgcc taccgctttg aactgaccgt taccggcggt tttgatgaag tacagaatgt
    15301 ttggcgtatc gtgcagaacg gcgtctgcgc ggttggtgcc cagttccata taggcgttat
    15361 cgatgttcgg gaactgacgc agatctttag ttttgatgtt tgctttcgcg taatcaacgg
    15421 agccagtgcc gctcttcacc gcaaccactt tcccgtcgag atctttcacg cttttcacat
    15481 cgttattgtt agctttcacc atcactaaca ggccgctttt gtagtagccg tcagagaaat
    15541 cgatcgcttt tttacgctcg tcggtgatgg taatgcccgc cagcgccaga tcgacgtttt
    15601 tggtttgcag tgccggaatg atcccactga aatccatcgg cttcagttcg taatccagct
    15661 tcagctcttt agcgatggca gcccacagat caacgtcaaa gcccacatat ttatcgccct
    15721 gtttaaattc aaacggaacg aaggcggtat ccgtcgcgac aactaatttt ttatccgcgg
    15781 catgagaaga aaccgcaaaa gccagggtca gtgcagccag tgaaactttt aatacagact
    15841 tcatagcatt tccttttttc tatccacggg acgatcccct gcgtgacatt cacatatatg
    15901 aaaaaatcgt gccagttttg caactcattg atttttcgag atgcggcaaa aacgtgatgc
    15961 acgatttata gggcaacacc ctgaagatgc accattctgg ggcaccaaac tggtgcgcta
    16021 aaattgtgca ctcaacacag tgattattta gcgtaaaacc tgatgaaaaa acaatcttta
    16081 tgtaacgatt gtgtgatgat gtggatacaa aaaatttact taatcagctg gggatagcag
    16141 atggatgcac taaataagtg cgttgaggcg ggctgcaaaa caaaacggcc tcctgtcagg
    16201 aagccgcttt tatcgggtac taaaattctg caccatcagc gatggattta ttcgatgtta
    16261 gactcgataa accacaggaa tttatccagg tcgcgagacg cggcggtcag gatatctgcg
    16321 gtgtcgtcat ctttcgcttc gccaatcgct ttgcgtacgt cattagcgac gattgcgtaa
    16381 cggtcagcca gttctttcag gtgatcctga acgttgtgga tgtccagcgg gtaacttttc
    16441 agcggggttt tgctgttgat aacttgagtg gtccccagag ctacaccgcc cagctgcact
    16501 gcacgttctg ccatggtatc cagatgatcg atcagtgcag tgcggaagcc atccagcatt
    16561 tcatgtacgg caatgaagtt agcgccgcgc atgttccagt gcgcttgttt ggtaatcaaa
    16621 gaaagatcaa taaactggat aacctggcga ttcagcaact ctactgttgc ttttttctcg
    16681 ctgtcagaga catcgttgcg ggtataaagc agattggtcg cttttgattt aactaattta
    16741 gcggtactca taatttcata tcctcttgat gttatgtccc agtaattaac gagactaagt
    16801 atagcaccgg ctatgtgttc cgctattctg gctgttccta tcacactaat agtggtaaca
    16861 agcgtgaaaa acaaaactaa taaagattca atgagttaga tatattgata agaacaattc
    16921 ttatttattt gctcgccagg aattttcgga ataaatatgc ttttgcataa ttcgatatat
    16981 aaagacggtg tagaggaaaa gtagcgagaa attctgcatg gttatgcata accatgcaga
    17041 aatgtaaatt taattaatgt ctaattcttt tattttgctc tctttgcgta ctgtcagcgt
    17101 agaacccatt gaagcggcga taattgcgcc gagcgccagc agctgtatgg gtgtcagtgt
    17161 ttctccgagg aaaatcatcc cggaaacggc agccagcgcc ggttccatgc tcatcagcgt
    17221 accaaatgtc cgtgttggca aacgggtgag ggcaatcatt tccagcgaat aaggcagagc
    17281 ggtcgagaga atagcgaccg ccagacccaa tggaataacc gaccagtgcc agagtgcttc
    17341 accagcctga agcgctccaa ttggcacgaa aattaacgct gcaatcaacg aaccaattgc
    17401 caccgtcgca gggccatgtt ccgctcctgc gcgttgccca cttaaaatgt aaatagccca
    17461 acaagccccg gcccccagtg ccagcgcaca gccggttaaa tcgacatggg aaacgtcttg
    17521 ccccagcggt agcaggaacc acagaccaag aaccgccagc acaacccaga cgaaatctac
    17581 cgggcgacga gaagagaaca gcgccaccgc cagtggtccg gtgaactcca gcgccaccgc
    17641 aatacccagc ggtaccgtct gaatagagag ataaaaaaga taattcatcc cacccagcga
    17701 aacgccgtaa aacaacagcg gtaaccgttg ctctttggca aagcgcagtc gccatggctt
    17761 aaagaacgcg atgaggatca gcgttcctaa tgccaggcgc agcgcagtga cacccggtgc
    17821 gcccaccaga ggaaaaagtg acttagctaa cgaagctcca ccttgaatag acgccatggc
    17881 aacgagcaat atgactattg gtaaccagac cggcatttta cgtaatgaac caggcatcct
    17941 ttctcccaca aatatctaga cttaagtaaa gcgtggagtg tactggatat acccaatgct
    18001 ggttgagcat ttgttgaaaa aattttcccc cgttttgact aaaatgcgcc aggattgatg
    18061 gaatcattag tctggtgatt aggaataatc tggatgaatg acagggaaaa catgcgtaat
    18121 acttacgcag ttctctgaaa aagtgattta aatttagatg gatagcggtg tatggaaacg
    18181 ttctgttaca tgaaatggcc cgttagacat cacaaatcgc gaagagtttc ccattaattt
    18241 ttgatatatt taaaacttag gacttatttg aatcacattt gaggtggtta tgaaaaaaat
    18301 tgcatgtctt tcagcactgg ccgcagttct ggctttcacc gcaggtactt ccgtagctgc
    18361 gacttctacc gtaactggcg gttacgcaca gagcgacgct cagggccaaa tgaacaaaat
    18421 gggcggtttc aacctgaaat accgctatga agaagacaac agcccgctgg gtgtgatcgg
    18481 ttcattcact tacaccgaga aaagccgtac tgcaagctct ggtgactaca acaaaaacca
    18541 gtactacggc atcactgctg gtccggctta ccgcattaac gactgggcaa gcatctacgg
    18601 tgtagtgggt gtgggttatg gtaaattcca gaccactgaa tacccgacct acaaacacga
    18661 caccagcgac tacggtttct cctacggtgc tggtctgcag ttcaacccga tggaaaacgt
    18721 tgctctggac ttctcttacg agcagagccg tattcgtagc gttgacgtag gcacctggat
    18781 tgccggtgtt ggttaccgct tctaatcact ttggtgatat aaaaaatccg cctctcgggg
    18841 cggatttttg tttttaaggc ttcgggtcga aaatatcggt tccgagatgg ttgtaatcca
    18901 ccttctgtaa ctggaagttg gtgatgtata tcggcccggc tttcttctca gatataaacg
    18961 ggtatttgat gttaatttcc ttcgctttaa ttcccgtcca ctgcgagaaa aagcctaaga
    19021 agtcattggc tgagcggcgg gctttaatca cccgatgcgc tttatcgtcg ctggaaatga
    19081 ccataaaagg cacctggaaa ttttgctgat acttatcatc atgggcaagg tattgcacgt
    19141 ctttgccgcg ctctttaaag gccagaccgt ggtcagaaaa gtaaaccagc gagaagctgc
    19201 tgccgctgtt gcgtaactga tcgtacagct tgcgcagtaa atcgtccgtt tgcgtcatag
    19261 tatagagata gcacgacgtt tctttcgatt gcacaaaggt ttcgtatttg ccttttgtcc
    19321 ggtcgcaggc ctgcggatgc gagcccatca gatgcagaac aatcagctgc ggttgcgagt
    19381 gctcttgcgc cagcacttga gcggtcatat ccagtaacgc ttcgtctttc gtgtttttat
    19441 ctgcttcaaa attaccttct ttcaggaagt acacttcatc tgcacgtttg gcgatgctgg
    19501 cgatagcagt atcgtattcg ccgatttggc cctggttgga aaaccaccag gtctggaagc
    19561 ccgcgcgatt tgccagggtg acaaagttat cctgaaactg tggtttgcca tcgactacgc
    19621 gattgagcgt taagccaagc gatttctgcg tggagccact ggcggcaatg tagtcagcaa
    19681 atatcaaacc gttaacgctg ctggcaaacg gggtattgtc ccagtgaccg ccaaaggcac
    19741 cgagggcatc gcgacgcgcg ctttcaccga tcaccaccac ataggtctga tacttcggct
    19801 taacggcagt gaccgtccag gtatctttca ttccggaaag ttttgccatc cgttcttgct
    19861 cttcaatcac ctcgttatta ttgacgatga catccttagc aaaacgaaat accggatagc
    19921 cagtatcttt cagtttaaat acgccacccc aggccaggtt ttgcacggga gcaacaaaaa
    19981 atgtcgccac actgaatacc agacacaggc tgtcgaaggt attccagcgc gctttttcat
    20041 tctctttttt tcgcctgatt gctattacgc cgagcgcaaa aataaataag ccgaccaggt
    20101 agctgtacca cggaaaaatc gtcaggattt cggtcgactc ttccatattg gtggagtgca
    20161 atgccagcaa tgtattgaaa ttaggcgcgc cgtaggcctg agcaaaaggg aaataacaag
    20221 ccgccaccag cgaactgaca ccgaccagaa ctttttgtac gcgaggcaat gttcgccata
    20281 gcaaaagcaa aatagccgta aacgcagcgg tgtagagcaa actgaagggg taacctaagc
    20341 cgaggttaat taatagcgac tgtaaaaagt agaacgcagt ccacgggcta aatacccggc
    20401 tacgggtaac aagcgattct ttgagggtta aattcatatg ccactatcgt aacaaacgcc
    20461 atgtgcttac cctggcgtca agggtcaata cctgcaagag tgcgtgaaga ggggaaaggg
    20521 gtccgcatcc gcgagccgca atatacgcag ggctgcaaga agatagagcg agccgcagtt
    20581 caggtcaact actgacgaac aaatcttttg cgaaggggat tgcaaatccg acaaaaagtg
    20641 gggtttttca gaccaaaacg cgtcgtgcct gcatgaaagc aggagtgaag cggcgtaccg
    20701 cgacgccgct atttatcgga aggtttatct tgctgcggtt tgttgttgac catatcgcac
    20761 aacatagaga gcagcattaa ccgtacttta aagggagaat gactaaacac gcgcatacac
    20821 ctcttgaact cattcataag acctcctgac ttgctaatcc cgtcgatcct tgagggatga
    20881 ttgcattaca tacagatata gcacaggcta tattatatag ctattgctaa aacgttaatt
    20941 ttttgtacct tcgcaactct ggtttacaat gtgcgcacga aatgagaacg ctatgtatgc
    21001 gtcaccataa tgaggaagca caatgagtcg tcgcgcaggt acgccaacag caaaaaaagt
    21061 gacgcagtta gtgaacgagg aagagcacgt tgaagggttc cgccaggtca gagaggcgca
    21121 tcggcgcgag cttattgatg attacgttga gctgatttct gacttgatca gggaagtggg
    21181 ggaagctcgt caggtagaca tggctgctcg tttgggagtt tcgcaaccga cggtggctaa
    21241 aatgcttaag cgactggcaa ctatggggct gattgaaatg atcccctggc gtggcgtgtt
    21301 tttaacggca gaaggagaga agctggcgca ggaaagccgc gagcgacatc agatagtcga
    21361 aaatttcttg ttggtgttgg gcgtcagtcc ggaaatcgcc cgtcgcgacg cggaaggcat
    21421 ggagcaccat gttagtgaag agacgctgga tgcctttcgt ttgtttaccc agaaacacgg
    21481 tgccaaatga gcctgctttt tttacgcacg ctgcaaggcg atcgtttttt tcagttatta
    21541 attcttgttg gtatcggatt aagttttttc gtgccctttg caccgaaatc ctggcctgct
    21601 gctatcgact ggcacaccat catcacctta agcggcctga tgctgctgac caaaggtgtg
    21661 gagttaagcg gttattttga tgtgctgggg cgcaaaatgg tgcgccgctt tgctacggag
    21721 cgtcggctgg cgatgtttat ggtgctggcg gcggcgctgc tttctacctt tctgaccaac
    21781 gatgtcacgc tgtttattgt tgttccgctg actatcacgc taaaaaggct gtgtgagatc
    21841 ccggttaatc ggctgattat ttttgaggcg ctggcagtca acgctggttc gctactgacg
    21901 ccaattggca acccgcaaaa tattcttatc tggggacgtt ctggtctttc gtttgccgga
    21961 tttattgccc aaatggcacc gctggctggc gcaatgatgc tgacgctcct gctgttgtgc
    22021 tggtgttgtt tccctggaaa ggcactccaa taccatacgg gggtgcaaac accggagtgg
    22081 aaaccgcggc tggtgtggag ttgtctgggg ctgtatatcg tctttctgac ggcgctggag
    22141 ttcaaacaag agctgtgggg actggtgatt gtggcggcgg gctttgcgct gctggcgcgt
    22201 cgcgtggtgt tgagtgtgga ctggacgctg ctgctggtgt ttatggcgat gtttatcgac
    22261 gtccatttac tgacccagct tccagcgttg caaggcgtgt tgggtaacgt gagtcattta
    22321 tctgaacccg gattatggtt aacggcaatc ggtttatcgc aggtgatcag taacgtgccg
    22381 agtactatat tgttgctgaa ctatgtgccg ccgtctttat tactggcatg ggcggtaaac
    22441 gtaggtggct ttgggttatt acccggatcg ctggcaaatt tgattgcgct acgtatggcg
    22501 aacgatcgcc gcatctggtg gcgtttccat ctctattcaa taccgatgct gttgtgggcg
    22561 gcgctggtgg gatatgtttt gttggttatg atcccggcct ggtaaggtct ggcaaataaa
    22621 aaaggcggat tatgagtccg cctttgtttt acgttttgaa gattaattca gacgaaccgg
    22681 catcccggag cggtttttaa tcgcttcatc aagaacaacc tggtcaacat ctggctggcc
    22741 ggtcactgtc tgcacgctct tcgtcagggt aattggcaca atttcctgac cttcaaattg
    22801 ggcttcggtg gtagacagcg ggttatggac ttcaatgtaa cggctgccgt ctggctcggt
    22861 ggtcgctttt accggctcat caataaactg tacgcgggta ccgaccggta ctttctcgaa
    22921 caggaatttg atgtcttcgt tacgcagacg cacacaacca tgacttacac gcagaccgat
    22981 accgaagtta gcgttggtgc catggatagc gtacaggcga ccgatgtaga gtgcatacag
    23041 ccccatcggg ttatccggac ctgccggaac gacagccgga agcggttcgc ccgcagcgcg
    23101 gtactctgcg tgcattttgg cggtcggcgt ccaggtcggg cctgctttct tacgctcaac
    23161 tttggtggtc cagttgatag gcgtatcttt gcctaactga ccaatgccga tcggcagcac
    23221 gataacggtg ttggtccctt tcggatagta atacagacgc atctctgcac tgttaatgac
    23281 gatgccttca tgaacggtat ccggcaggat cagctgctgc ggaatgttca gtacagtacc
    23341 gcctttcggc aggaaggtat ccacacccgg gttcgcttcc atcatattgg aaagccccat
    23401 ctggtactcc gcggcaaaat actccagcgg ctgagtgtta ccttcaggaa tggtgatcac
    23461 ctgattctga ccaaccaggc gactcccgtc ggttggcaga ggataagtta ccgcagaggc
    23521 ggtactgcaa aagccgacaa cagcgaaggc cgctgcgaat aatgttttca atttcatatt
    23581 catgttaagc gagattttgt gcctggcagg ccattgggtt gagaatatta gagtattgga
    23641 agcgcattat aagttcattc cagctcacag tgaaatcaga tgtgtacgaa atcacatttt
    23701 ttgcctttgg cttgagtgta gatcttaagc gaggagcagg atcttctttc agacttatgg
    23761 cataatgcgc ggtttgtcat atctcttttc aggatacgcc tgtgttagtt tccagtaacg
    23821 tcaccatgca gttcggcagt aagccgttgt ttgaaaacat ttccgtcaaa tttggcggcg
    23881 gcaaccgtta cggcctgatt ggcgcgaacg gtagtggtaa atccaccttt atgaagatcc
    23941 tcggcggcga ccttgagccg acgctgggta acgtttccct cgatcccaac gagcgcattg
    24001 gtaaactgcg tcaggatcag tttgcctttg aagagttcac tgtgctggat acggtgatca
    24061 tggggcataa agagttgtgg gaagtgaagc aggagcgcga ccgcatctat gctttgccgg
    24121 aaatgagtga agaagacggc tataaagtgg ccgatctgga agttaaatac ggcgaaatgg
    24181 acggttactc tgcggaagct cgtgccggtg aattgttgct tggcgtggga attccagtgg
    24241 aacagcacta cggcccgatg agtgaagttg ctcctggctg gaagctgcgt gtgttgctgg
    24301 cgcaggcgct gtttgctgat ccggatattc tcctgctcga cgaaccgacc aacaacctcg
    24361 acatcgacac cattcgctgg ctggaacagg tgctgaacga gcgtgacagc accatgatca
    24421 tcatctcgca cgaccgtcac ttccttaaca tggtctgtac ccacatggcg gatctggatt
    24481 acggcgagct gcgcgtttat ccgggtaact acgatgagta catgacggcg gcgacccagg
    24541 cgcgtgaacg tctgctggcc gataacgcca agaagaaagc gcagattgct gagctgcaat
    24601 ctttcgttag ccgctttagc gccaacgcct cgaaatctcg ccaggcaact tcgcgcgcgc
    24661 gccagattga taaaatcaaa ctggaagagg tgaaagcctc cagccgtcag aacccgttca
    24721 tccgttttga acaggataag aagctgttcc gtaacgcgct ggaagtggaa ggtctgacca
    24781 aagggtttga taacggtccg ctgtttaaaa atctcaacct gctgctggaa gtcggtgaaa
    24841 aactggcggt actgggtacc aacggcgtcg gtaaatcaac gctgttgaaa acgctggtgg
    24901 gcgatctgca accggacagc ggcaccgtaa aatggtctga aaacgcgcgc attggttact
    24961 atgctcagga ccacgaatat gagtttgaaa atgatctgac cgtgttcgaa tggatgagcc
    25021 agtggaagca ggaaggcgat gacgagcagg cggtgcgcag tattctcggt cgtttgctgt
    25081 tcagccagga cgacatcaaa aagcctgcta aagtgctttc cggtggagaa aaagggcgga
    25141 tgctttttgg taagttaatg atgcagaagc cgaacattct gatcatggac gaaccgacca
    25201 accacctgga tatggaatcc atcgagtcac tgaacatggc gctggaactg tatcagggca
    25261 cgctgatctt tgtttcacac gaccgtgagt tcgtaagctc cctggcgacc cgcattctgg
    25321 aaatcacccc ggaacgcgtg atcgacttta gcggtaatta cgaagattac ctgcgtagta
    25381 aagggatcga gtaagttatt atccgtcacc ccgatactgt cggggtgacg gcggctgatg
    25441 acaaacgcaa gattgcctga tgcgctatgc ttatcaggca ttaagaaagc gcacttgtca
    25501 gcaatctgag taacgtcgaa tcgattttct taatcccaca tcaattcact tcaattaact
    25561 ctcaaacatc catgcccaac gctcgcttac cgtgaatgtt gaggtcggca agcgtaaagc
    25621 gtccttccca gtttgtttca tagtcctcgc gcgggaagtc gcccggcgat gcgccttttt
    25681 ccagtgcggc cttcaccttg tgcgcgtagg caagattttt ctcgcacatc ggtgccgcag
    25741 gaatgtacat cacgttgccc caaccctgtt gattttcaac gggggcaacg gaatggatga
    25801 cgtcgcagtg ccaccagacg gagtctccgg cctcgagttt tggaatgctg gttaacgcct
    25861 caattaacag tggatgccat tgctctgata ccggcaacac tcttccgggc gctacgccgc
    25921 acagttcatc ctccggcaca tcatcaagca gcggacgtaa cagtacgtac gccatggctt
    25981 caggaatggg cacgacgtgc agcaacccct gactaggcag catatcagag agcgctgtcc
    26041 agccctggaa tgtccgaaac acggaacatt tggtggtgtt gtccaccgtg tactcttcaa
    26101 cttccgtacg atgtgccgca tgccagggat cgtatttcgc cagattgcca ttaaagacgt
    26161 tagcgaaaac gtgctgatac gctggaagca gccagcgttc cagcgccccg gagtcggtat
    26221 gcgctccaag acctttggag gtcgttccgg gcggacggcg gcggatacgg tcagggtaga
    26281 tgacgctcac atccgggtta aaccattgct ttccatcact ttcaaatgtc cacagacgat
    26341 tgagaaacga ctgcgcattc gccatttctt cactctggcg ggcctgcatt tgcgcctgcg
    26401 accagtagat ggggtaaatc tcgggacgtg aagcgctgag cgtcccgaag aaattatcgc
    26461 cggggccttt gtagacctcg tcaaagcggt tgcggtccag atagtccagc atcgactgat
    26521 cccagcctag cgcttgttcg cgggggaaat ggccttttat caccgcacaa ccgcgacgtt
    26581 taatctgttc gcgctgctct gcagtaacat gacctgcttt gatatcggca taagacagca
    26641 ccggccagac ggcatcgccc tgtgctttga gtgcgttgat ttcagccact cgcgtggcaa
    26701 tgtcatcgct tagctgatta aagatctgct ggacgtcgcc aagctgcgcc cgcagcgcgt
    26761 gcttcatctg acggatagct gctttgtgat cggcaggcaa tgtgtcgctg gtaaaagtag
    26821 aagccataac cacctcgcat taactttcat tcgaaactat actgatttgc atgtgacaat
    26881 ataagttata aataagttaa tgcaagttta atgatttgat gtgatgtaca ggatgtaaaa
    26941 agaagggata accggagcgc atggctccgg tagagaggtc agctgttaaa aggggatgtg
    27001 ttatccagta cagcctgaat cacattcagc gcgccttcat gattattatc atcggtagcg
    27061 taacgggcga tttgtttaat gttttccgca gcattgccca tcgcaaagga ataacgcgcc
    27121 attttcagca tctccgcatc gttaccgctg tcgccaatcg ctaccacatt ttgcggtgac
    27181 agatcccagc gtttcagtaa ccgcgaaata ccgtttgctt tatgtagacc gggaataatc
    27241 aggtcgataa agccaaaacc actggtgacg ggtttcataa tgccatcgag cgctatgtgc
    27301 agtttgtcga tcactaacgg gatttgttca tccggcaggt tgagtgaaaa cttgaacagt
    27361 acgtcgtcaa tctcctgata atcttttaca ggtttcaggc gatggtagtg ttttgccatc
    27421 agtgcgacaa atgcttcggg ggcattttcg ctgacatatg cactttgcag accgcaggcg
    27481 acaaaattga gttgcttatc ttttagcaac tcgccaataa caatccgcga ttcatgtcgg
    27541 gtcagttcgc cgtggaacag ttgcttgcca tgttcgtaaa ccagtgcgcc gttttccgcg
    27601 acaaaagaga tctcatcctt tagctcagga aagaatgaaa taagctggta atactgatta
    27661 ccgctggcga caacgaactc aatgccgcgc tttttcagtt cctgatattg cgccataaaa
    27721 cgtggttggt tgtacgtttt gacgtcgtta agaaaagtac cgtccatatc tgtgacgata
    27781 acttttacgc tcataggtgt gctcccggct cgaaaatgaa accgtaacag tgtaataaca
    27841 atgtgacgca gagcacaaat tatatttcga atgaaagtaa ggatgaaatt gatgatgtga
    27901 atgatttagc ccggcgacgt ctccgccggg ctgaggggat tacagcatat gttcggtacg
    27961 ggcgatgata tcgtcctgag cgtctggaga gagcgcggtg aagaaggcgg aataacccgc
    28021 gacacgcact accagatcgc gatactgatc gggatgtttt ttcgcctcca gcagcgtttc
    28081 gcgggaaacg atgttgtact gaatatgcca gcctttatgc acttcaaaga aggtgcgcag
    28141 caggatcatc agtttctgct tgtcagattc gttctccagt gttgctggat tcagtttctg
    28201 gttgagcaac accccgccga gaatcgctgc cgtaggcagt ttacccaccg agccaatgac
    28261 cgcagtaggg ccaagatgat cagtaccgga agccgggctt gcgccttctg ccagcggggt
    28321 gtgggcttta cgtccgtctg gcgttgccat agtttgcgcg ccaaacggta cgttagcgga
    28381 gatagacgac gtacccgcgt aatagttgcc gccaaccgga ccacgaccgt agcgcgggtt
    28441 atggtactgt ttcagttcgt cgatataggt ctgataagcg cgagccagca gcgtatcgac
    28501 agtatcatcg tcgttgccgt acttcggcgc accgttaatc agccgctgac gcagctgctc
    28561 gtgagtcagg ccgtcgaagt cattcgccag tgcggcagcc agctgttgct gactaatcgc
    28621 gccctgttca aataccagtt tcttcacggc cgccaggctg ttgccgaggt tggcaatgcc
    28681 aacctgcagg ccagaaaccc agtcatattt cgcgccgcct tgcttgatac ttttcgctcg
    28741 ctcaatacag tcatccacca gcgccgagca gagaatatcg tgcacgttct cttccagcat
    28801 ggtgtcgacg acatattcga tttcgattga tttacgcgtg tagtaacgga tttgcgtatc
    28861 ccacgcgtcc atcacttcat cgaagttgtt gaagttacct gccgacaacg ctttttcttg
    28921 tggcaggaac actttgccgc tggtggcatc acgcccgcct tccagcgccg ccagcatcac
    28981 gcgggcgaag ttgataaagc tcatgccggt acagcgatag ccccatttgc caccgacggc
    29041 ggtttcgata caaccaatcg ctgcatagtt gtaagcatcc tgcggttcaa taccgagttt
    29101 aataaattcc gggatgacga tttcgtcgtt gttgaacgcc ggcatcccga agccgcaacg
    29161 gatcacctgt acgcaggcgt cgaggaaatc gttgctcatt cccgcgtggt aacgcacgct
    29221 gaggttaggc tgagtggaac gcaggcgacc gcaggattcg aggatcgcgt aagagagtgg
    29281 attcaccgcg tccattggtt gaccatcaac cagattttgt ccgccaatgg tgacgttctg
    29341 atacagagga cttcccgcag aggcttttga gtgtgagcct gagcggattt tgttcacttc
    29401 cagcagtttc agccagcagc tatgcagcaa ttcgatggcg tgttcgcgat ccagtgtctg
    29461 gttgagttca acgtcgcggc gatagtacgg gtagagatac tggtccatac gaccaaacga
    29521 tactgagtga ccgttagatt cgatctgcaa aatcaactgg atgaagtaac acagttgcag
    29581 cgcctgccag aaagtctgcg gcggctggtg ggcgataaga tcgcagtttt ctgccatcgc
    29641 cagcagttca tcgcgacggc tttcgcgggt ttcggtcgcg gccattgcac gcgccagggc
    29701 agcgaaacgt tcaatgtgtt cactgactgc caccagcacg atatcaatcg ctttcaggaa
    29761 ctgttcgccg tgcagatctt ccagtaccgt cagattgatg cgcgagcgac gttccgctac
    29821 tttctcgcgc agaccatcaa gcctttttcc agcagcagcg ggaaattcac cgccaggtgc
    29881 gcatcgccgg aggtcatatt gccttccgct ttaatgattc cggtcgccag cagacctttt
    29941 tgctcatcgg taaacatgcc gtagcagcga tcctgtaccg tctgaccgcg ccaccacggg
    30001 cacacttcat gcagaacgcg tttgttctct tcgctcaccg caaagccagc accgggacga
    30061 tctgccagat catcaatctc tttttcgatc cacgagacag tatattccgg gaagatcggc
    30121 gcggcgcgaa cttcgcttgc ctggttgccg atgatcaact catcgtgctt gatccagata
    30181 gtgcgattcg ccaggtgatg tgccagcgcc agcgcgcgac gtacagggat cggcttatcg
    30241 agatgttgtt gatacatctc ggtatagtgc tgcgcgcgct cggtacaaac cggcggtttc
    30301 acaatatgca ccagcgcatt tttgtgcgct ttaatgcggt cgctgagcgt gtccagtttc
    30361 agtgtggtca tggttgttat cctcgtaagg tcgcggttaa ccctttctgg caggcatact
    30421 gctgggcaaa gtcgagcagt tctggcgcat caagcggttt ttccggggcg tcatagggca
    30481 gattaagtaa gtgatatttg ttgatgccca gcgtgtggta gggcaggaaa tgaatttcgc
    30541 caacgtgcag ctcgtcggcg gcaaaatcgg taatggcttt cacagaggtt tcgtcggcat
    30601 taaagccttg aatcagcggc acgcggataa tgattttttt gcccgctgcg gcgagttttt
    30661 tcaggttatc cagcaccctg gcggcgttac cgtcggtcca ctgtttaaac ggcgcgtcgg
    30721 caacgtgttt taaatcggca agaaacagat cgatataggg cagagaaggg gcgatatatt
    30781 tccacggcac atgcaggcag gtttctaccg cagtatgaat gccggcctcg tggctggctt
    30841 gcagtagcgc cgtcgccatt tccggctgca tgaatggttc accgccagaa agtgttaagc
    30901 cgccgccgct gcgatcgtaa aacggtttat cgcgcagaac ggtcaccatg atctcctcaa
    30961 cgcttttcac ttccccacac acggttaacg actgtgtcgg gcagcagtcg gttaacgccg
    31021 tcagatgctc cggggttaac ttttcccgat gaataagcaa accattcagc gcgcgctcaa
    31081 tcacttccgg cgcggtctta gcgcacagct cacagccttc caggcacaga cgtgggtcat
    31141 acagcagatc ctgcgtgcgg gcgcggcttt ccgggttctg acaccagcgg cagcccagcg
    31201 aacagccttt aagaaatacg accgtgcgga taccggggcc atcatgggtc gagtagcgct
    31261 gaatattgaa aatcatagtt gcctctctgt ttcgttcaaa tattaaaata ctttcgaatg
    31321 aaagttagat tgatgtgcgt caactgttca gagagttttc ccgtgatagt ctacattcag
    31381 acaaaaagta cattttgagg atggttatgg aactgtatct ggatacttca gacgttgttg
    31441 cggtgaaggc gctgtcacgt atttttccgc tggcgggtgt gaccactaac ccaagcatta
    31501 tcgccgcggg taaaaaaccg ctggaggttg tgcttccgga acttcatgaa gcgatgggcg
    31561 gtcaggggcg tctgtttgcc caggtaatgg ctaccactgc tgaagggatg gtgaatgacg
    31621 cacgtaaatt gcgttctatt attgcggata tcgtggtgaa agttccggtg accaccgagg
    31681 ggctggcagc tattaagatg ttaaaagcgg aagggattcc gacgctggga accgcggtat
    31741 atggcgcagc acaagggctg ctgtcggcgc tggcaggtgc ggaatatgtt gcgccttacg
    31801 ttaatcgtat tgatgctcag ggcggtagcg gtattcagac tgtgaccgac ttacaccagt
    31861 tattgaaaat gcatgcgccg cgggcgaaag tgttagccgc gagtttcaaa accccgcgtc
    31921 aggcgctgga ctgcttactg gcaggatgtg aatcaattac tctgccactg gatgtggcac
    31981 aacagatgat tagctatccg gcggttgatg ccgctgtggc gaagtttgag caggactggc
    32041 agggagcgtt tggcagaacg tcgatttaac cggacgttct gcatcctcat aaattgctga
    32101 tgacgtggcg gagtgcaccg atgcctgccg cgcttggaaa tctgtgcccg aaccgtaggc
    32161 cagataaggc gttcacgccg catctggcgt ctggagcaaa tgcctgatgc gacgcttgtc
    32221 gcgtcttatc aggcctggag gtggcaatta ctgcccgcac acctcacacc ccggattgcg
    32281 catcagtttc atttcgcgaa actgacaggt catcgcatcg tacatgacga ttttcccgct
    32341 ggccggtttg ccataacctg ccagcagttt gatcgcttcc attgcctgca acgaaccaat
    32401 cacgccgatc aacggtgcca tcacgcctgc ttctacgcag gttaacgcgt tctcgctaaa
    32461 caaacggcta aggcagcgat agcacggttc gccgtcctga taagtgaaga ccgtgatttg
    32521 cccttccata cgaattgccg cgccggaaac cagcggcacc ttcgcggcaa aacagcctgc
    32581 gttcagttga ttacgtaccg caacgttatc cgtacagtcg agcacaagat tgtgttcagc
    32641 aatcaatgct gcaagttctg cgtcatccag cagtgcattg actggcgtaa tcgcgatatg
    32701 tgggttgatc cgcgtcagag cgtcacgggc ggattccacc ttcggttgcc cgaccgtagc
    32761 atcactgtgc agcgtctggc gttgcagatt cgagagcgaa accgagtcga agtcgagcag
    32821 cgtcaggttg ccgacaccgg cgcttgccag atactgcgaa gctgcgcagc cgagtccacc
    32881 gaggccgacg accagcacac gagaatcttt cagcgcctcc tgaccgtcaa aatcaaaacc
    32941 gcgcagaatg atttgccggt tgtagcgcag catctcttga tcgctgagtt ccgccattac
    33001 aggcctccga acaacgcgtt aaacggttct acttccaccc attcgcccac ttccacattg
    33061 ccgcgatcgc gttccagcac gataaagcag ttgccgaggc taaaggagct gaatatatgt
    33121 gaaccctgat gtccggtggt cgtcacttcc agttcgccat cggcgttgcg gtgcagcacg
    33181 ccgcgctgga aatcaagacg tcctggcgtt tttttcaggc gggacgctgt gcgtacgcgc
    33241 tggcgcgcgg gcaggccgct ggcggtgttg ccgcttagtt ttgccagcaa aggctgtacc
    33301 agttgataga aggtcagcgt cgctgaaacc gggttgcccg gcaggccgca gaaccagcta
    33361 ttgctgagtt taccgaacgc gaacggttta cctggtttaa tcgccagctt ccagaaggcg
    33421 atctccccca gctcttcaag aatcgttttg gtgtaatccg cttcacccac tgaaacaccg
    33481 ccggaactga tcaccacatc cgcctggctg tcggcttcaa taaatgcggc gcgcagggca
    33541 tgtggatcgt cgcggataat ccctaagttg attacctcgc aacccagctg ttctaacatc
    33601 aggtgtacgg cgagacggtt ggtatcgtag atttggccgt cgcccagcgg ctggcctggc
    33661 aactggagtt catcaccggt agaaaaaagc gctacacgca ctttacgaat caccggaact
    33721 tcggcaatcc ccagtgaagc aattactggc agctctgcgg tagtcaggcg agtccccgcc
    33781 gggaaaacaa ccgcacctgc agagatatct tcaccgcgac ggcgaatatt ttgcccgcta
    33841 cgcacttcag cagtaaaacg cacgccattg tccgtttgtt cagtctgctc ctgcatcacc
    33901 accgcttcgc agccttccgg caccggcgca ccggtcataa tacgaatgca ggtacccgca
    33961 ggccattcac catggtacgg ctgaccggca aaggatttac cggcaacggg cagcggttgt
    34021 ccggaggtga tatcggccaa acgtaccgcg taaccatcca ttgcggagtt atcaaaccct
    34081 gggacatcca gcggcgaaac gacatcgctc gccagaatac gaccaaaaca ctgaaccagt
    34141 ggcagcgttt cctgggcggt cagtggggtg acgcgagaaa gcatctcatt aagcgcggtg
    34201 tcgagcgaca tcaatccggt ggtaaattcc atgaaaacac tcctgcggag gcaaaatcga
    34261 atttgcctat tatgtcagaa aaacgccaca gactgtatgc cacctcgggc gtagcgctgg
    34321 gtcctgcctt tacatgccat atccatcttt ctatattcaa aaattgaatg agtaattcat
    34381 aaaaattctg atatttatag caaaagtggc gagccaccct taatggacga atactatggg
    34441 caaagcagtc attgcaattc atggtggcgc aggtgcaatt agccgcgcgc agatgagtct
    34501 gcaacaggaa ttacgctaca tcgaggcgtt gtctgccatt gttgaaaccg ggcagaaaat
    34561 gctggaagcg ggcgaaagtg cgctggatgt ggtgacggaa gcggtgcgtc tgctggaaga
    34621 gtgtccactg tttaacgccg gaattggcgc cgtctttacg cgtgatgaaa cgcatgaact
    34681 ggacgcctgt gtgatggatg gtaacaccct gaaagccggt gcggtggcgg gcgttagtca
    34741 tctgcgtaat ccggttcttg ccgcccggct ggtgatggag taaagcccgc atgtgatgat
    34801 gattggcgaa ggggcagaaa attttgcgtt tgctcatggc atggagcgcg tctcaccgga
    34861 gattttctcc acgcctttgc gttatgaaca actaatggca gcgcgcgagg aaggggcaac
    34921 agtcctcgac catagcggtg cgccactgga tgaaaaacag aaaatgggca ccgtgggggc
    34981 cgtggcgttg gatttagacg gcaatctggc ggcagccacg tccacgggcg gaatgaccaa
    35041 taaattaccc ggacgagttg gcgatagccc cttagtgggt gccggatgct acgccaataa
    35101 cgccagtgtg gcggtttctt gtaccggcac gggcgaagtc ttcatccgcg cgctggcggc
    35161 atatgacatc gccgcgttaa tggattacgg cggattaagt ctcgcggaag cctgcgagcg
    35221 ggtagtaatg gaaaaactcc ctgcgcttgg cggtagcggt ggcttaatcg ctatcgacca
    35281 tgaagggaat gtcgcgctac cgtttaacac cgaaggaatg tatcgcgcct ggggctacgc
    35341 aggcgatacg ccaaccaccg gtatctaccg tgaaaaaggg gacaccgttg ccacacagtg
    35401 atgaacttga tgccggtaat gtgctggcgg ttgaaaatct taatattgcc tttatgcagg
    35461 accagcagaa aatagctgcg gtccgcaatc tctcttttag tctgcaacgc ggtgagacgc
    35521 tggcaattgt tggcgaatcc ggctccggta agtcagtgac tgcgctggca ttgatgcgtc
    35581 tgttggaaca ggcgggcggt ttagtgcagt gcgataaaat gctgttgcgg cggcgcagtc
    35641 gtgatgtgat tgaacttagc gagcagagcg ctgcacaaat gcgccatgtg cgcggtgcgg
    35701 atatggcgat gatatttcag gagccgatga catcgctgaa cccagtattt actgtgggtg
    35761 aacagattgc cgaatcaatt cgtctgcatc agaacgccag tcgtgaagaa gcgatggtcg
    35821 aggcgaagcg gatgctggat caggtacgca ttccggaggc acaaaccatt ctttcacgtt
    35881 atccgcatca actctctggc gggatgcgcc agcgagtgat gattgcgatg gcgctgtcat
    35941 gccgcccggc agtgctgata gccgatgagc caaccaccgc gctggatgtc actattcagg
    36001 cgcagatcct gcaattaatc aaagtattgc aaaaagagat gtcgatgggc gttatcttta
    36061 tcactcacga tatgggcgtg gtggcagaga ttgccgatcg ggtactggtg atgtatcagg
    36121 gcgaggcggt ggaaacgggt accgtcgaac agatttttca tgcaccgcaa catccctaca
    36181 cccgtgcgct gttagctgct gttccgcaac ttggtgcgat gaaagggtta gattatcccc
    36241 gacgtttccc attgatatcg cttgaacatc cagcgaaaca ggcccccccc atcgagcaga
    36301 aaacggtggt ggatggcgaa cctgttttac gggtgcgtaa tctggtcacc cgttttcctt
    36361 tgcgcagcgg tttgttgaat cgcgtaacgc gggaagtgca tgccgttgag aaagtcagtt
    36421 ttgatctctg gcctggcgaa acgctatcgc tggtgggcga gtctggcagc ggtaaatcca
    36481 ctaccgggcg ggcgttgctg cgcctggtcg aatcgcaggg cggcgaaatt atctttaacg
    36541 gtcagcgaat cgataccttg tcacccggca aacttcaggc attgcgccgc gatattcagt
    36601 ttatttttca ggacccttac gcttcgctgg acccacgtca gaccatcggt gattcgatta
    36661 tcgaaccgct gcgcgtacac ggtttattgc caggtaaaga agcggttgca cgcgttgcgt
    36721 ggttgctgga gcgcgtgggc ctgttacctg aacatgcctg gcgttacccg catgagtttt
    36781 ccggcggtca gcgccagcgc atctgcattg ctcgcgcgtt ggcattgaat ccaaaagtga
    36841 tcattgccga cgaagccgtt tcggcgctgg atgtttcaat tcgcgggcag attatcaact
    36901 tgttgctcga tctccagcgt gatttcggca ttgcgtatct gtttatctcc cacgatatgg
    36961 ctgtggtaga gcggattagt catcgtgtgg cggtgatgta tctcgggcaa attgttgaaa
    37021 ttggtccacg gcgcgcggtc ttcgaaaacc cgcagcatcc ttatacgcgt aaattactgg
    37081 cggcagttcc ggtcgctgaa ccgtcccgac aacgaccgca gcgtgtactg ctgtcggacg
    37141 atcttcccag caatattcat ctgcgtggcg aagaggtggc agccgtctcg ttgcaatgcg
    37201 tcgggccggg gcattacgtc gcacaaccac aatcagaata cgcattcatg cgtagataac
    37261 attcaggcgg agaataaaat ggcaagagct gtacaccgta gtgggttagt ggcgctgggc
    37321 attgcgacag cgttgatggc atcttgtgca ttcgctgcca aagatgtggt ggtggcggta
    37381 ggatcgaatt tcaccacgct cgatccgtat gacgcgaatg acacgttatc tcaggccgta
    37441 gcgaaatcgt tttaccaggg gctgttcggt ctggataaag agatgaaact gaaaaacgtg
    37501 ctggcggaga gttataccgt ttccgatgac ggcattactt acaccgtgaa attgcgggaa
    37561 ggaattaaat tccaggatgg caccgatttc aacgcagcgg cggtaaaagc gaatctggac
    37621 cgggccagcg atccggcgaa tcatcttaaa cgctataacc tgtataagaa tattgctaaa
    37681 acggaagcga tcgatccgac aacggtaaag attaccctca aacagccgtt ctcagcgttt
    37741 attaatattc ttgcccatcc ggcgaccgcg atgatttcac cggcagcgct ggaaaaatat
    37801 ggcaaggaga ttggttttca tccggtggga accggaccgt atgaactgga tacctggaat
    37861 cagaccgatt ttgtgaaggt gaaaaaattc gcgggttact ggcagccagg attgcccaaa
    37921 ctggacagca taacctggcg tccggtggcg gataacaaca cccgcgcggc aatgctgcaa
    37981 accggtgaag cgcagtttgc tttccccatt ccttacgagc aggccgcact gctggagaaa
    38041 aacaaaaata tcgagttgat ggccagtccg tcaattatgc agcgttatat cagtatgaac
    38101 gtgacgcaaa agccattcga taacccgaag gtccgtgagg cgctgaatta cgccattaac
    38161 cgtccggcgc tggtgaaagt ggccttcgcg ggctatgcaa cgccagctac tggtgtggta
    38221 ccgccgagta tcgcctacgc gcaaagttat aaaccgtggc cttacgatcc agtgaaagcg
    38281 cgcgaattac tgaaagaggc gggatatccc aacggtttca gtaccacgct gtggtcgtca
    38341 cataaccaca gcaccgcgca gaaagtgctg caatttaccc agcagcagtt agcgcaggtc
    38401 gggattaaag cccaggtgac tgcgatggat gccggacagc gggcggcaga agttgaaggt
    38461 aaagggcaaa aagagagcgg cgtgcggatg ttctacactg gctggtcggc ttcaaccggc
    38521 gaagcggact gggcactatc gccgttgttt gcctcgcaaa actggccacc gacgctgttt
    38581 aataccgcgt tttacagcaa taaacaggtg gatgacttcc tggctcaggc actgaaaact
    38641 aatgatccgg cggaaaagac ccgcttatat aaggcggcgc aggatatcat ctggcaagag
    38701 tcgccgtgga tcccgctggt ggtagaaaaa ctggtgtcgg cacacagtaa aaacctgacc
    38761 ggtttttgga tcatgccaga taccggcttc agctttgaag acgcggattt gcaataagca
    38821 acgcagggag tggaatgctt aattacgtta tcaaacgatt actggggttg attccgacgc
    38881 tgtttatcgt ctcggtgctg gtgtttttat ttgtgcatat gctgcccggc gatccggcgc
    38941 gattgattgc cgggcccgaa gctgatgcgc aggttataga actggtgcgt cagcagctgg
    39001 ggttggatca gccgctgtat caccagttct ggcactatat cagcaatgct gtgcaggggg
    39061 attttggcct gtcgatggtg tcacgtcgtc cggttgccga tgagattgcc aggcgcttta
    39121 tgccaacgct gtggctgacc ataaccagta tggtctgggc ggtgatattt ggtatggcgg
    39181 cgggaattat cgccgccgtc tggcgtaacc gttggccgga tcgattgagt atgaccattg
    39241 cggtgtcggg gatctcgttt ccggcatttg cgctggggat gcttttaatt caggtattct
    39301 ccgttgaact gggctggctg cctaccgtgg gagcagacag ttggcagcac tacattttac
    39361 cctccctgac gctcggcgcg gcagtggccg ccgtgatggc gcgctttacc cgcgcgtcgt
    39421 ttgtcgacgt tttaagcgaa gattatatgc gtaccgcgag ggcgaaaggg gtgagcgaaa
    39481 cctgggttgt cctcaaacac gggctacgca acgcgatgat cccggtagtg accatgatgg
    39541 gcttacagtt tggctttttg ctcggtggtt ccatcgtggt ggagaaagtt ttcaactggc
    39601 ccggacttgg acgcttactc gttgactccg tagaaatgcg cgattacccg gtgattcagg
    39661 cggaaattct gcttttctcg ctggaattta ttcttatcaa cttagtggtg gatgtgcttt
    39721 acgccgccat taacccggct atcaggtaca agtaaggatg cgactattta actggcgacg
    39781 tcaggcggtg ttaaacgcca tgccactggt caaacctgac caggtacgta caccgtggca
    39841 tgaattctgg cgacgatttc gccgtcagca tatggcgatg accgccgcat tattcgttat
    39901 tttattgatt gtggtggcca tttttgcacg ctggatcgct ccctatgacg ccgaaaatta
    39961 ttttgattat gacaatctga ataacggacc ttctttgcag cactggtttg gcgtcgattc
    40021 actggggcgc gacattttca gccgtgtcct ggttggtgcg caaatctcgc tggcggcggg
    40081 cgtgtttgcc gtgtttatcg gtgcggcgat agggacgttg ctgggcttgt tggctggata
    40141 ttatgaaggc tggtgggatc ggctgatcat gcgcatttgc gatgtgctgt ttgccttccc
    40201 gggtatttta ctggcgatcg ctgttgttgc ggtgttggga agcggcattg ctaacgtgat
    40261 tattgcagtc gccatttttt ccatccctgc gtttgcccgc ctggtgcgcg gcaacacgct
    40321 ggtgttgaaa cagcaaacct ttattgagtc agcacgcagt attggtgcca gcgatatgac
    40381 cattttgttg cgtcatatcc tgcctgggac cgtctcttct atcgtggtgt ttttcaccat
    40441 gcgcattggt acctcgatta tctctgccgc cagcctgtca tttctcggcc tcggtgcgca
    40501 gccgccgaca ccagagtggg gagcaatgct caatgaggct cgagcggata tggttatcgc
    40561 gccgcatgtc gctgtttttc cggccctggc tatttttctg actgtactgg cgttcaattt
    40621 gttgggcgat ggtttacgcg atgcgctgga tccgaaaatt aaaggatagt tacgtttgaa
    40681 tattgcttga aagagtaatc acctcacagg aaattattgc cctaagcaag tgttgtaact
    40741 ttctgctgat tttgtagaat cgggtaattt ggttaaatag ccgcagcaag ggactgtttt
    40801 tgcagcggca cagcgttcag acagttattt tgttaaatgt attaacatg
//
LOCUS       DAZFTN010000033        39375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFTN010000033 DAZFTN010000000
VERSION     DAZFTN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            230..1885
                     /gene="aslA"
                     /locus_tag="ACHKV2_001887"
     CDS             230..1885
                     /gene="aslA"
                     /locus_tag="ACHKV2_001887"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO6939779.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2053..3288)
                     /gene="aslB"
                     /locus_tag="ACHKV2_001888"
     CDS             complement(2053..3288)
                     /gene="aslB"
                     /locus_tag="ACHKV2_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6939780.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3435..3511)
                     /locus_tag="ACHKV2_001889"
     tRNA            complement(3435..3511)
                     /locus_tag="ACHKV2_001889"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3475..3477),aa:Pro,seq:tgg)
     gene            complement(3554..3640)
                     /locus_tag="ACHKV2_001890"
     tRNA            complement(3554..3640)
                     /locus_tag="ACHKV2_001890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3604..3606),aa:Leu,seq:cag)
     gene            complement(3661..3736)
                     /locus_tag="ACHKV2_001891"
     tRNA            complement(3661..3736)
                     /locus_tag="ACHKV2_001891"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3701..3703),aa:His,seq:gtg)
     gene            complement(3795..3871)
                     /locus_tag="ACHKV2_001892"
     tRNA            complement(3795..3871)
                     /locus_tag="ACHKV2_001892"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3835..3837),aa:Arg,seq:ccg)
     gene            complement(3974..5359)
                     /gene="yifK"
                     /locus_tag="ACHKV2_001893"
     CDS             complement(3974..5359)
                     /gene="yifK"
                     /locus_tag="ACHKV2_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6939781.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5550..6290)
                     /gene="wecG"
                     /locus_tag="ACHKV2_001894"
     CDS             complement(5550..6290)
                     /gene="wecG"
                     /locus_tag="ACHKV2_001894"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO6939782.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6293..7645)
                     /gene="wzyE"
                     /locus_tag="ACHKV2_001895"
     CDS             complement(6293..7645)
                     /gene="wzyE"
                     /locus_tag="ACHKV2_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO6939783.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7642..8721)
                     /gene="rffT"
                     /locus_tag="ACHKV2_001896"
                     /gene_synonym="wecF"
     CDS             complement(7642..8721)
                     /gene="rffT"
                     /locus_tag="ACHKV2_001896"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO6939784.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8718..9968)
                     /gene="wzxE"
                     /locus_tag="ACHKV2_001897"
     CDS             complement(8718..9968)
                     /gene="wzxE"
                     /locus_tag="ACHKV2_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO6939785.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9970..11100)
                     /gene="rffA"
                     /locus_tag="ACHKV2_001898"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9970..11100)
                     /gene="rffA"
                     /locus_tag="ACHKV2_001898"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO6939786.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11105..11779)
                     /gene="rffC"
                     /locus_tag="ACHKV2_001899"
                     /gene_synonym="wecD"
     CDS             complement(11105..11779)
                     /gene="rffC"
                     /locus_tag="ACHKV2_001899"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO6939787.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11757..12638)
                     /gene="rfbA"
                     /locus_tag="ACHKV2_001900"
     CDS             complement(11757..12638)
                     /gene="rfbA"
                     /locus_tag="ACHKV2_001900"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6939788.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12657..13724)
                     /gene="rffG"
                     /locus_tag="ACHKV2_001901"
     CDS             complement(12657..13724)
                     /gene="rffG"
                     /locus_tag="ACHKV2_001901"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6939789.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(13724..14986)
                     /gene="wecC"
                     /locus_tag="ACHKV2_001902"
     CDS             complement(13724..14986)
                     /gene="wecC"
                     /locus_tag="ACHKV2_001902"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO6939790.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14983..16113)
                     /gene="wecB"
                     /locus_tag="ACHKV2_001903"
     CDS             complement(14983..16113)
                     /gene="wecB"
                     /locus_tag="ACHKV2_001903"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6939791.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16169..17215)
                     /gene="wzzE"
                     /locus_tag="ACHKV2_001904"
     CDS             complement(16169..17215)
                     /gene="wzzE"
                     /locus_tag="ACHKV2_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO6939792.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17227..18330)
                     /gene="wecA"
                     /locus_tag="ACHKV2_001905"
     CDS             complement(17227..18330)
                     /gene="wecA"
                     /locus_tag="ACHKV2_001905"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO6939793.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18570..19829)
                     /gene="rho"
                     /locus_tag="ACHKV2_001906"
     CDS             complement(18570..19829)
                     /gene="rho"
                     /locus_tag="ACHKV2_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO6939794.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19914..20015)
                     /gene="rhoL"
                     /locus_tag="ACHKV2_001907"
     CDS             complement(19914..20015)
                     /gene="rhoL"
                     /locus_tag="ACHKV2_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO6939795.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20156..20485)
                     /gene="trxA"
                     /locus_tag="ACHKV2_001908"
     CDS             complement(20156..20485)
                     /gene="trxA"
                     /locus_tag="ACHKV2_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO6939796.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20616..21881
                     /gene="rhlB"
                     /locus_tag="ACHKV2_001909"
     CDS             20616..21881
                     /gene="rhlB"
                     /locus_tag="ACHKV2_001909"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO6939797.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            22017..23501
                     /gene="gppA"
                     /locus_tag="ACHKV2_001910"
     CDS             22017..23501
                     /gene="gppA"
                     /locus_tag="ACHKV2_001910"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO6939798.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23548..25569)
                     /gene="rep"
                     /locus_tag="ACHKV2_001911"
     CDS             complement(23548..25569)
                     /gene="rep"
                     /locus_tag="ACHKV2_001911"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO6939799.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25786..26235
                     /locus_tag="ACHKV2_001912"
     CDS             25786..26235
                     /locus_tag="ACHKV2_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO6939800.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26434..26715
                     /gene="ppiC"
                     /locus_tag="ACHKV2_001913"
     CDS             26434..26715
                     /gene="ppiC"
                     /locus_tag="ACHKV2_001913"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO6939801.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26761..28236)
                     /gene="ilvC"
                     /locus_tag="ACHKV2_001914"
     CDS             complement(26761..28236)
                     /gene="ilvC"
                     /locus_tag="ACHKV2_001914"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO6939802.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28386..29279
                     /gene="ilvY"
                     /locus_tag="ACHKV2_001915"
     CDS             28386..29279
                     /gene="ilvY"
                     /locus_tag="ACHKV2_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO6939803.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29331..30875)
                     /gene="ilvA"
                     /locus_tag="ACHKV2_001916"
     CDS             complement(29331..30875)
                     /gene="ilvA"
                     /locus_tag="ACHKV2_001916"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO6939804.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30878..32728)
                     /gene="ilvD"
                     /locus_tag="ACHKV2_001917"
     CDS             complement(30878..32728)
                     /gene="ilvD"
                     /locus_tag="ACHKV2_001917"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO6939805.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32793..33722)
                     /gene="ilvE"
                     /locus_tag="ACHKV2_001918"
     CDS             complement(32793..33722)
                     /gene="ilvE"
                     /locus_tag="ACHKV2_001918"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO6939806.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33742..34005)
                     /gene="ilvM"
                     /locus_tag="ACHKV2_001919"
     CDS             complement(33742..34005)
                     /gene="ilvM"
                     /locus_tag="ACHKV2_001919"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO6939807.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(34002..35648)
                     /gene="ilvG"
                     /locus_tag="ACHKV2_001920"
     CDS             complement(34002..35648)
                     /gene="ilvG"
                     /locus_tag="ACHKV2_001920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO6939808.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35651..35701)
                     /gene="ilvX"
                     /locus_tag="ACHKV2_001921"
     CDS             complement(35651..35701)
                     /gene="ilvX"
                     /locus_tag="ACHKV2_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO6939809.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(35788..35886)
                     /gene="ilvL"
                     /locus_tag="ACHKV2_001922"
     CDS             complement(35788..35886)
                     /gene="ilvL"
                     /locus_tag="ACHKV2_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO6939810.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36239..37759
                     /gene="yifB"
                     /locus_tag="ACHKV2_001923"
     CDS             36239..37759
                     /gene="yifB"
                     /locus_tag="ACHKV2_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO6939811.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37784..38122)
                     /gene="maoP"
                     /locus_tag="ACHKV2_001924"
     CDS             complement(37784..38122)
                     /gene="maoP"
                     /locus_tag="ACHKV2_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO6939812.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38241..39080
                     /gene="hdfR"
                     /locus_tag="ACHKV2_001925"
     CDS             38241..39080
                     /gene="hdfR"
                     /locus_tag="ACHKV2_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO6939813.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(39176..39251)
                     /locus_tag="ACHKV2_001926"
     tRNA            complement(39176..39251)
                     /locus_tag="ACHKV2_001926"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39216..39218),aa:Trp,seq:cca)
     gene            complement(39260..39336)
                     /locus_tag="ACHKV2_001927"
     tRNA            complement(39260..39336)
                     /locus_tag="ACHKV2_001927"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39300..39302),aa:Asp,seq:gtc)
BASE COUNT         9644 a        10718 c         9917 g         9096 t
ORIGIN      
        1 aaaaacagca tcaataatca acgcgatata ataaacctgc cttacatatc aactgcgcca
       61 gaggtaggat tgaaaacgct ctcctgattt ttaaattcat ttcttggata aataaataat
      121 ttatttttat cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca
      181 atgcttaatt acgtcatcat tttgttgaca tgaaaccgga gcattttgca tggaattttc
      241 gttttcaccc aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc
      301 agcagatacc ccgtcaactg ccaccgcgcg caaaggcttt gccggatacg atcacccaaa
      361 ccagtatctg gttaaaccgg cgaccactat tgccgacaac atgatgccag taatgcagca
      421 tccggcgcag gataaagaac cccagcagaa gctggcagaa cttgagaaaa aaaccggtaa
      481 gaaaccgaat gtggttgttt tcttgctgga cgatgtgggc tggatggacg tcggttttaa
      541 cggtggcggc gtggcggtgg gtaaccctac accagatatc gacgccgttg ccagccaggg
      601 gctgatttta acttcggcgt attctcaacc aagctcttcc ccaacccgcg ccaccattct
      661 caccggacaa tactccatcc accacggcat tctgatgccg ccaatgtacg ggcaaccggg
      721 cgggctgcaa gggttaacca cgctgccgca gttgctgcac gatcagggct acgtcactca
      781 ggccatcggg aaatggcata tgggggaaaa caaagagtcg cagccgcaga acgttggctt
      841 tgatgatttc cgtggcttta actcggtgtc tgatatgtac accgaatggc gcgacgttca
      901 cgtcaatccg gaagtggccc tgagtccgga ccgttctgaa tacatcaagc aattaccatt
      961 tagcaaagat gacgttcatg cggtgcgcgg cggcgaacaa caggccattg ccgacattac
     1021 gccgaaatat atggaagatc tggatcaacg ctggatggaa tatggcgtta agttcctcga
     1081 caagatggcg aagagtgata agcctttctt cctctattac ggcactcgcg gctgtcactt
     1141 cgataactac ccgaacgcca aatatgcggg tagctctccg gcacgcacct cgtatggcga
     1201 ctgcatggtg gaaatgaacg atgtgttcgc taatctgtat aaagcactgg agaaaaacgg
     1261 tcagcttgat aacacgctga ttgtctttac ctccgataac ggaccggaag ccgaagtacc
     1321 gccgcacgga cgcacaccgt tccgtggtgc gaaaggatct acctgggaag gcggcgttcg
     1381 cgtaccgact ttcgtttact ggaaaggcat gatccaaccg cgtaaatctg acggtattgt
     1441 tgatctggca gatctcttcc caacagcact ggatctggcg gggcatcctg gtgcgaaagt
     1501 ggcgaattta gtgccgaaaa ccacctttat cgatggtgtg gaccagacat ctttcttcct
     1561 gggaacaaat ggtcagtcta accgtaaggc cgagcactac ttcctcaacg gtaaactcgc
     1621 tgctgtgcgt atggatgaat tcaagtatca cgtcctgatt cagcaacctt acgcttatac
     1681 ccagagcgga tatcagggtg gattcaccgg cacagtaatg caaacagccg gatcgtcggt
     1741 gtttaacctc tacaccgatc cgcaggagag cgactctatc ggcgtgcgcc atattccgat
     1801 gggtgtaccg ctacagaccg aaatgcacgc gtatatggag atcctgaaaa agtatccacc
     1861 acgcgcgcag attaaatctg attaagccgg cgctatcgct gagatctgcc tttgccggat
     1921 gcgatgctga cgcatcttat ccagcctaca gaacgctgca atttattgaa tttgcacgat
     1981 catgtaggcc ggataaggcg tttacgccgc atccggcaat caaccgcagg cgaccacaaa
     2041 atggccgctt ttttacttac tcaccaccag caaatgcgcc tgcataatgt cgctggccgg
     2101 gcgaccgtgc gccagcaaat cagccattgc tttaagatat ggcggtagat ggcggaaata
     2161 acgctgatac ccggcacaca aataattcag tcccggtttg ccgctggcat cgagcatgaa
     2221 gcggtgtttc gggcagcctc cccaacatgc ttttaacacg ttacaactgc gacactgcgc
     2281 cggtaattgc ttaaatttat cttcaccaaa cacctgctgt tgcggggaat cgatcatttc
     2341 tgcaattgtt tgctggtgca tattccccag tcgatattgc ggataaacat agtgatcgca
     2401 ggcgtaaacg tcgccattgt gctcaacaat caccgagcgc ccacaggttg gctgatgatg
     2461 gcaaaccgca cccggcgcac cgacaaaatt ggcaaacgcc cattcgatat tcatcacgaa
     2521 aatcttgccg acgtcgcgtt taatccagtg gtcgaatatc gccaccagaa attcaccaaa
     2581 ttcatcgggg cgcacagacc attccgtcag ttccccctga atatcaccag gcgcatgtag
     2641 tttcagtcct tcgcgggcag tcgtttcatc agccaggcgc tcgaccaccg gaataaactg
     2701 gatgaattcg actcccgcat cgcacaaaaa atcataaacc tgtaacggtt gctgcgcgct
     2761 ggtgcggttg acgcagacca gcacgttata gtcgacatga tgtttttgca ggagcgtcag
     2821 ggcacgcatc accagtttat gcgtgggtct gccgcctttt gtcacgcgat attgattgtg
     2881 gatctcagcc ggaccatcca gcgataaccc aacaagaaaa tgattttctg ccagaaatgc
     2941 acaccattca tcatcgagca gtacgccgtt agtctggaag ctgttactta tcttcctgcc
     3001 agcaccatat ttcgcctgta gcgccacggc acggcggtaa aactccagcc cgagtagcgt
     3061 tggttcgccg ccctgccagg taaaagccac ttcgttttgc ggttcgctgg cagcgatata
     3121 gtgacggaca tacgcttcca gcgtgtcatc gtccatatgc gtgactggct tttcgcggta
     3181 aagggattgt ttttcgagat aaaaacagta gtcacagttc agattacaat cggaaccgct
     3241 cggtttcgcc atcacatgaa aagcacgcgt tggaacctgt tgcagcatgg tcgctccttg
     3301 cctgaagtca tccttcaata cgttaagggc gaaatcatac cgtggggcaa tgagttatta
     3361 cgggcgttta ttgcgaaggg agggtgacga aatgcacaga aaacaaaaaa ccccgccgaa
     3421 gcggggttca aaattggtcg gcgagagagg attcgaacct ccgacccact ggtcccaaac
     3481 cagttgcgct accaagctgc gctactcgcc gaaatactgc tttttgaatt tttagttcaa
     3541 ttctttaaag tcgtggtgcg agggggggga cttgaacccc cacgtccgta aggacactaa
     3601 cacctgaagc tagcgcgtct accaattccg ccaccttcgc attgtcacaa cttctaataa
     3661 tggggtggct aatgggattc gaacccacga caactggaat cacaatccag ggctctacca
     3721 actgagctat agccaccact acaaatcttg ttacgcggta ttactaccac cgcagctcaa
     3781 gcgccgggac taaatggcgc gcccgacagg attcgaacct gagacctctg cctccggagg
     3841 gcagcgctct atccagctga gctacgggcg cttagcgccg ttgcgggggt ggataatacg
     3901 gacttccccc ccctctgtct agtccctttt taaataaatt gcgcgtttgg ttacggtttg
     3961 tgcgttttgc tgcttattcc tccagtttat gcgctttccc gtggcgatta aggccaaaaa
     4021 ctttataaat cgccgtcacc gccagcataa agatgatgcc aacaaacagc gacatacgcg
     4081 tatcttcatt aaagtacatg ccgatcaaaa cgcaaatcag gaatgccatt gttacgtagt
     4141 tggcccacgg gaacaggatt gagcggaacg gatggctggc aatcgccgct ttatgtgcac
     4201 gccgaaaacg cagctggctt atcaatatca caaaccatgg caccatcccc ggaagcacgc
     4261 tggcactgta gacgtagaca aacacacgct gcggattggg aatgatgtag ttcaggcatg
     4321 agccaattag cagaatagca atagatactg ccacacccgc aaccggtacg ccgtgacggg
     4381 aaactttcgc catcgccgcc ggtaactgac ggtttttcgc cagtgcgtag agcatacgtc
     4441 cgcaactgta catgccgctg ttacagccag agagcgcagc cgtcagcacc acaaagttga
     4501 taatgcccgc cgctgcggta ataccgattt tggcaaaagt cagtacgaac gggctgccgt
     4561 tgctgcctat ttcattccac gggaagatgg tgacgataac gaaaatcgcg ccaacgtaga
     4621 aaatcaggat ccgccacagc accttgccta cggcactgcg cagcgtcacc tgcggattct
     4681 tcgcttcacc ggcagtaatg ccaatcagct ccacgccctg gtaggacgcc accacaatac
     4741 acagcgcggt caggaaccct ttccagccac ccgcaaagaa accgccatgc tctgtgagat
     4801 tgctaaaacc aatcgactgc ccgccattgc caaagccaaa gaaaatcacg cccaggccaa
     4861 tgacaatcat cacgataatc gtggtgactt tgatcatcgc gaaccagaac tcgatttcgc
     4921 cgtacaaccg caccgctgcc agattcgcca acgccaccag cgccactgcg atcaatgcgg
     4981 gtatccactg cgccatctcc gggaaccaga actggacata aacgccaatg gcggtgattt
     5041 cagagatccc caccgccatc cacataaacc agtaagacca ggcggtgaga tagccaaaga
     5101 acgggctcat ataacgatgc gcataaacgg cgaacgaacc ggtaaccggt tcgaggaaca
     5161 acatttcgcc cattgaacgc atgatgaaaa agacgaacag cccggcgatg atataggcca
     5221 acaatacgga tggcccggcc catttcaggg tactggcggc ccccataaac aggccgacgc
     5281 caatggtgcc ccccagggcg atgagttcga tatgtcgagc ttccagccca cgctgtagct
     5341 ctggtttgtt atctgccata aatcctcgtg ttgtgtttgc atgctttccg gtgttaccgg
     5401 ttatcgttat gggtacatcg agtgttgcga atgttttcgt aattcaggag aaatggcaaa
     5461 taaagcatta aaaatttgaa tgctttgtgt aataaaaaag cagacaggcg acggagtgac
     5521 cactccgtcg ctttacaaag agaggaaaat cagaggttgc cggtgtaatg ccagcgtaaa
     5581 taacgcagca aacgaagctg acgcttaatg cggctcggct gcgaaagcag gcggtagagc
     5641 cactccagcc ccagcgtttg ccagattttc ggtgcgcgtt ttacgtgacc ggtgaaaaca
     5701 tcgtaagtcc cgccaacgcc catatacagc gcatctggat gcaccagacg gcagtcgcgc
     5761 atgaagatct cctgctttgg cgatcccatc gcaacggtga cgatttgcgc tccgctggca
     5821 tgaatgcgtt caaacagcgc ctgacgctgc tcgggtttaa aataaccatc ctgactgcca
     5881 acgatattca cattccactg gttgcgcagt ttagcttcag tttgcgccag cacttcaggt
     5941 ttaccgccca caagaaatac cgtcgtccct tctttgcctg cgcgcgccat cagctcttcc
     6001 cagagatcgg caccggcaac gcgggaaacc tgcgcctgcg ggtacttttt acgtactgaa
     6061 cgtacaacgc tgatgccatc cgcatattta aattcggcag cgttaattaa ctccctgacc
     6121 tcggcgttct cttcaatggt cagcattttt tcagcattaa tggcaaccag cgttccctgc
     6181 ttaagctgcc cgtcagcaaa cagataatcg agggcatgct gcatatcacg ccaaccaatc
     6241 aactgtaagc cacgcagcgt ataggtcggt gccgtggtgt tgttattcat tgttatcctt
     6301 caacctgcgt ccggagcgat gattttgtac gtttatgaat gagtccggcg ctttcaaaaa
     6361 gccagtacaa cagttttgcg atcatcagac atgcgccgaa gaccacgata aaaaagacca
     6421 cgcgtgagac aaacgaatcc agcccttcac gcgccagcac gatcatattg aagatcgccc
     6481 caaagcagaa actgtgcaat atcgcagcct tatagcgatt agtttcgcgg ttgcccagct
     6541 catacagcca gtcgaaccat ttgatgatca gtccaaccac aatcgccccg agcgggatga
     6601 acaacgcgcc gcccatcacc accagtgagc ctataagcgt aggcgagatc gccagtccgg
     6661 agtggttatt cagcacttcc caggtaaagt agttggctga gttcagcacc atactcgggc
     6721 gacccggcca cagccaggaa gggataaaga catagaaatc gcggacaatt ggcgccaggc
     6781 cctggaagtc gatgttgtcg tagttctgca acagcaacgc cagattctcc cacggcgaga
     6841 aggtgtcgcg agtgagatag agaaacgtat agaacgcttc atcgccgctc acattcattc
     6901 catagcgttt tagcgccagc cagaacatgc caacaatccc cagcacgccc gccgccgcca
     6961 gcatccacaa cgaaatccag ccgcgaataa tgccaataaa caggaagata gcgaatgcga
     7021 tgatgatatt ggcgcgagtg ccgccgacaa tcatataagt cagcaagcca aaggcgaccg
     7081 tgctgacgag gaaaaacagc cacgctttgc tgtcctggcg cagaaagtag accaccagca
     7141 tcgccgggat gaaaaagtaa aagaagcgtt ttaacgccac gccggagact tcactggaaa
     7201 agatctgact gtaggagtta agccggaaca gcaaaaagcc gttgtgcatg aagaagatgc
     7261 cgacgcttac cagcgcgata cccatcagga tcacccacgt aagattggtt tccacgcggt
     7321 tcatggtaaa cagcggacgg cgcggtacat cagcaacgcg tttgcgtagg cgggttttgt
     7381 aggtgacgta gtaaaccgcg tagaagcagc ccgcagaaag caacgcctgc aacaggattt
     7441 ctggcggcgc gacaccaaca tcaaagcgaa acaccagcac gctggtcagc gggaagccaa
     7501 agaaaaaggt gagcagaaac agcaatgaaa agaatacatt gaagttaaag cgcacacgac
     7561 gaaactcaaa ccaggtcagc gtggcaataa acagcgtgca gagcagccag acaacaaaca
     7621 ggccactgaa ttgcagcaga ctcatgcgac ctccccggcg gcaatcgcca acgcccgctg
     7681 ccagccttgt agatagttag ggctaaagaa ggcaatggtg tttttatcca ccgacgccaa
     7741 ctggcgctgc gcttcacgca caatatcctc gttgagatcg tcggtagtaa acagcaccgg
     7801 taaatgctgt tccgtcatat cctgccagaa cggattttcc cggttaagca cacaaggaat
     7861 gcccgcctga atcagtaagc acagcgtacc aatgccctgc tggcgggcaa aaataaagta
     7921 accaagatcg cactgacgaa gtagcgtcag ataggcgtca aattccagtt tttcactcag
     7981 aacttgcaga ttttcttcgc tgaataactc cagccccgcc tgacgaactt cctcgatgta
     8041 cgcttcgtta tgaggcggat atcccatcgg caccaccact tttaccgtat cgccaaattg
     8101 ctgatgaacg gcgcgcaagg cagcaatatg ctcattgctg cggtcgccgg agttccccac
     8161 cagaatggtc attttccctt cacgttgccg atcgttcgcc atcgtattga gagaagcatc
     8221 catccgcgtc gggaagtaca gcagttcgcc ccgcaccttt gggtgcgttt tggcaaaaaa
     8281 gctcaaatca ccacgggtgg caaatacaca gccgactcgc ttttgcgcca ggcgacgtag
     8341 tgggtaaaaa agcttatatc tcaagccact ggaaagctcg tacaggtctg ccccccagat
     8401 atgccagtaa aactggctgg gcttaatgcc accactcagc agagccagcc acagtgtggg
     8461 attgaactga ccgtggaaga aaaaacgctg ctgacggtta gcttttgctt tcgcgatgac
     8521 cgcttccgcc agcgattttt tcccagggaa aaattgcaca gaaagcgccg gacagctatc
     8581 acttaagccg tcgtccttgc caacaaccat aaactcgcgc gcgtgctcgc tcgtcgcggc
     8641 cagcgcgtca ttgaaaaacc gcaaaacggt tcggttatgg tgagggatat ccgatcccag
     8701 tacgtgaatc agtacagtca tgcccgccta cgccagagta aaaacacgcc acaacaaaga
     8761 gaaaaataga cgatataggt tgccatatat gcctgtgccg cgcccagcgc gccgtgcgta
     8821 gggattagcc agtgggcaaa taccatcaat aaagtgaact ggctgacttc cgccagaata
     8881 taaaaccgca gtgaagcttt ggcgatcacc agataaccaa agacataagc gcccactttt
     8941 aacacatcac ccaccaactg ccaggcaaag agatcgcgca tagcggtaaa tttattcgac
     9001 aacagcagcc agatagcaaa atcacgcagc aaccagacgg taaaactcgc cgccgccact
     9061 gccggtaaga cgaatttcag cgatttaacc acttcccggg tgatatcgcg cttttccgtt
     9121 agccgcgaca acgtgggcag caaatatacg ctgaacgatg ccgtaataaa ttgcaggtag
     9181 gcatcggaaa tactgctcac cccttgccag atccccacct catcccagct atactgcgcc
     9241 gccagcagtt tacgcatcat gatgtaagca acaggcaagg tcaccgacgt aatcaacgtc
     9301 atgagcgtaa atttgctcaa ctgccctgcc agaccgttat cccagctggg tttcagatag
     9361 cttaacggga tagcaccacg cttaattaac atgacggcgg caggaatcac caccagcgcg
     9421 ggaatcaacg ccagacccag caacgcccct tcatatccgc ccaaacggta actgacgtag
     9481 tacgcaagaa cgccaatcaa gctgccaaca atcagagata acgcattacc tgcggcatcg
     9541 cgaaagcctt tcatcaacgc cagtaacagg ttgccccagg cgatccccat ttgcaccagc
     9601 gccactaaac gcaccagccc ctgatagtcg tgattaccaa acaatcccaa gctgattggc
     9661 gcagctgcca gcacaaaaac cagcgccatc agcgtagaga agccaagtac catcgctgat
     9721 gaagtgccga ccacgcggcg cagctgttgc ggattatcat ggtactgggc aacgtatttg
     9781 gttacaccgt taaagatgcc agccccggca agcacgccga gcacggtaat caactggcgg
     9841 aaatttgccg ccagcccaag ccccgccgga ccaaatgaca ccgccagcaa cttaccgacc
     9901 agtaacccgg caccaatctt gaccagtgta ctggccgccg tccacaagga cgcttttgcc
     9961 aacgacatat caggagaagt agttcaacaa agtcgcaatt accgtacgct gattgacggg
    10021 cgacaggttg tagaacagcg gcaggcgcag caggcgctcg ctctctttgg tggtgtagcg
    10081 atcttcaccg tggaactcac caaagcgttc ccccgcaggg caaccgtgca gcggaatgta
    10141 atggaacacc gccatgattt ccgcttcttt cagaaagtta atcaacgcgc tccggtcacc
    10201 aatatcccgc agtttaatgt aaaacatatg cgcgttctgc aagcagccat cgggaatcga
    10261 cggcagctcg atacgcccgg ctttcgccag aggtgctaac gcatcgtagt agttttgcca
    10321 cagcgccaga cgttgctggt tgatacgatc cgctgcttcc agttgcgccc acaggtacgc
    10381 agcttgcaga tcggacatca aatagctgga gccgatatcg cgccaggtat atttatcgac
    10441 ctgaccacgg aagaactggc tgcggtttgt gcctttttca cggatgatct cggctcgttc
    10501 gatcaacgct ttatcgttaa tcagcgtcgc gccgccttca ccgcccgccg tgtagttttt
    10561 ggtttcatgg aagctaaagc agccaatatg accaatggtt cccagtgcac gccctttgta
    10621 agtggacatc acgccctgag cagcatcttc caccacaaac agattatgct ttttcgccaa
    10681 cgccataatg gtgtccattt cgcaggccac gcccgcgtaa tgcaccggca cgataacgcg
    10741 cgttttgtcg gtgatcgccg cttcaatcaa cgtttcgtcg atgttcatgg tgtccgggcg
    10801 aacatccaca aaaacgattt ttgcgccacg cagcacaaag gcattggcgg tggagacaaa
    10861 ggtgtagctc ggcatgatca cttcatcgcc aggctggata tcgagcagca gcgccgccat
    10921 ctccagcgaa gcggtgcagg acggcgtcag taacactttg gcgctgccaa aacgttgctc
    10981 cagccactgc tggcagcgac gggtaaaacc gccatcgcca cacagtttgc cgctacccat
    11041 tgccgactgc atatagtcga gttcggttcc caccaccggc ggtgcgttaa atggaatcat
    11101 gtgatcacct gtataaccag tacgcggtgc tttctacatt cgcaccactt tgtatgtatc
    11161 gtttaagcgc ggcggtgttg cccatttggg tcgccacccg caaagttgtt ttaccgcgag
    11221 catacgccca gtttagcgcc gtttgcatca gctcagcacc tgcaccgcgt ccagccagca
    11281 ggccaattcg cgcatctgta gcattgagtt cccgtaaaga gacataaccg cgaatatcgc
    11341 cggacgccgc acgtaaaatc agacattgat gatcaaaggt gccgcgcacg gcattttcaa
    11401 tccactgtgc ataaaagcga ctgctggcgt caggcgcata ccacggcgca cgaaaacggc
    11461 tttgcgcaaa tgcggcgctg gctaactgac gtaatgcggg aatatcggtc tcttgtgcca
    11521 ctacagcacc gctatcactg gcattgttca cgggtagcgc caaatcaact tcaccttcta
    11581 ccagggagaa tcccagctgt tgcagggcat ccagttcccc cgtatttgat gccgcaattt
    11641 tggcctgcac ccgtgaccac ggcgctaacg cgtctggcgt caggaacggt gcttcagacg
    11701 taatgcgcac gatggcgctg ttaacaccaa agaaggcgtt ttcccaggtt agtggctcaa
    11761 tactggcgcg gacgggcacg aagtaactcc agcagatatt ggccgtagcc agttttcgct
    11821 aatgaactgg cagcacgctt cacaccctcg tcatcgagcc agccgttacg ccaggcgatc
    11881 tcttccaggc aggcaatctt aaagccctgg cgtttttcca ccgtctgtac aaaggtgctg
    11941 gcttcaatca ggctgtcgtg agtgccagtg tccagccagg caaatccgcg cccgagcagt
    12001 tcaacggtca ggttgcccgc ctcgaggtac atctggttga tggaggtaat ctccagttca
    12061 ccacgctccg acggcttcac ctgctttgcg tactccacga ctttactgtc gtagaaataa
    12121 agcccggtca ccgcccagtt tgatttcggc tgttttggct tttcttccag cgagatagcg
    12181 cggaaattat cgtcaaactc caccacgcca aagcgttccg ggtccatcac ctgatagcca
    12241 aaaaccgtcg ccacttccgt gcgcgccgca acatgacgca gcttcggact gaagccctga
    12301 ccgaagaaga tgttatcgcc cagcaccaga caagaaggtt caccattaag gaaggtttca
    12361 ccgatgataa aggcctgcgc cagaccgtcc gggctgggct gttcggcata ttccagctgg
    12421 ataccgaact cactaccatc gcccagcagg cgctggaaat aacctttatc ttccggcgta
    12481 gtgatgatga gaatttcgcg gataccggcc agcatcagca ccgacagcgg atagtaaatc
    12541 attggcttat cgtaaatcgg caacagttgc ttcgatacgc cgcgcgtaat cggatgcaat
    12601 cgggtaccgg aaccgcccgc caggataata cctttcatgt ttgcctccgc tgaaaattag
    12661 cctttcagac ctaaacgctc gccctgatag ctgccgtcct gcacctgctt cctccagctt
    12721 tcattagcca gataccactg caccgtttta cgcattccac tttcaaaggt ttcctgcggc
    12781 agccagccaa gttcacgggc aattttcgaa gcatcaatgg catagcgcag atcatgcccc
    12841 ggacggtcag cgacaaaggt gatcaagtca cgataatgca ccacgccttg cggcttgttc
    12901 ggagccagtt cttccagcag ctcgcaaatg gtttccacaa catcgagatt cttacgctcg
    12961 ttgtgaccac caatattata ggtttcaccg actttcccgg tggtcgccac gcaatacagc
    13021 gcgcgggcgt gatcttcaac atacagccag tcacggattt gctgcccgtt gccatatacc
    13081 ggtagcggtt tacccgccag cgcgttgagg atcatcagcg ggatcagttt ttccggaaag
    13141 tggtaaggac cgtagttatt cgagcagttg gtgataagcg ttggcagacc ataggttcgc
    13201 agccaggcgc gcaccagatg gtcgctgctg gcttttgacg cggaataagg gctgctcggc
    13261 gcatacggcg tggtttcggt gaagaaatca tccgtcgagt gcaggtcacc atatacttcg
    13321 tcagtggaga tatgatgaaa acggaacgct gattttttat cttccgtcag cgcattccag
    13381 taagcccgcg ccgcttcaag caatgtataa gtcccgacaa tgttggtttc aataaacgct
    13441 gccgggccgt caatagaacg gtcaacatgg ctttcggctg ccagatgcat gacacagtct
    13501 ggctgatgct cagtgaatac gcgcgccagt tctgcccgat cgcagatatc aactttctca
    13561 aaggcaaagc gctcgctttg cgcgaccggt gccagcgaca tcaggtttcc ggcgtaggtc
    13621 agcttatcga ccactaccac cgcgtcgctc gtttcgttga tgatataacg caccagcgcc
    13681 gagccaataa acccggcacc gcctgttatc agaatctttc tcatcagcgc cagactcctt
    13741 tagcatcgac gacatactgc tgatggacat tgtcgccatt gataactttg aactgactat
    13801 gatcgaccag catcaccagc acgtctgccg ttgccagcgc ctcgtcaagc tgcgccagag
    13861 tacaaagccc ggtcagtttt ttcggcaact ggtggatgtt aggctcaaca accagagttt
    13921 cgccgctatg ccactgggcg atcagttcag cgatttccat cgccgggctt tcgcgcaggt
    13981 catcaatatt cggtttaaac gccagaccaa agcaggcgat tttcagttca ctggcgcgtt
    14041 tatcggtagc cgccaggcaa tcagccaccg ccgctttcac ctgatcgata acccagaacg
    14101 gtttgtgatc gttcacttcg cgcgcggtac ggataagccg cgcctgctgg gggttctgtg
    14161 ccacgataaa ccacggatca acagcaatgc agtgaccgcc cacgccaggg ccaggctgaa
    14221 gaatattaac gcgagggtga cgattcgcca ggcgaatcag ttcccagacg ttaatcccct
    14281 gatcggcaca aatcagcgac aattcattag caaaagcgat attcacatcg cggaagctgt
    14341 tttcggtgag cttacacatt tccgccgtcc gcgagttagt gacgacacac tcaccttcga
    14401 ggaaaatttt gtacagttcg ctggcgcggg ccgaacaaac cggcgtcata ccaccaatca
    14461 cgcgatcgtt tttaatcagc tcgaccataa cctggcccgg taacacgcgt tccgggcagt
    14521 aagcaatgtt gacgtccgcc tgctcgccca cctgctgtgg gaaagtgaga tccggacgca
    14581 tctctgctaa ccattctgcc atcttctcgg ttgaccccac cggcgaggtg gattcaagga
    14641 tcaccagcgc gccttttttc agcactggcg caatggagcg agcagccgat tcaacgtagg
    14701 tcatatctgg ctcatgatcg cctttaaacg gcgtgggtac agcaatcagc caggcatccg
    14761 cttcaactgg cgtcgtgctc gctcgtaaaa aaccgccttc tacggcagtt tttactacgc
    14821 tcgccaaatc aggctcgacg atatggattt cgccacgatt gatggtatca accgcgtgtt
    14881 ggttgatatc gacaccaatc acctgttttt gccgtgaggc aaacgctgct gctgtaggca
    14941 gcccgatgta acccagtccg ataacagaaa tggtcgcaaa actcatagtg atatccgatt
    15001 attttttaac gcttccagaa tgcgagagca tgcctgacca tcaccatacg ggttatgggc
    15061 gcggctcata gtttgatatt cgttttcgtc ttttaaaaga cgcgtcactt cctcgacaat
    15121 tcgctgctta tccgtgccta ccagacgcac cgtacccgcc gtcaccgctt ccggacgctc
    15181 agtggtatcg cgcatcacca gcaccggttt ccccagcgaa ggcgcttctt cctgaatgcc
    15241 gcctgagtcg gtcaaaatca gccaggcgtg gttcatcagc cagacaaacg gtaaatactc
    15301 ctgaggatcg atcagaatga catttttcac atgccccaga atgcgattga ccggctctct
    15361 gacgttcggg ttgagatgca ccggatagac aatctggatg tcctggtgcg tggtggcgat
    15421 gtctgccagc gcgtggcaga tttcttcaaa gccacgaccg aagctttcac gcctgtgacc
    15481 ggtcaccaga atcatctttt tatcggggtc gataaacggg taatttgccg caagttctga
    15541 acgcagcgtg tcgctgctca tcacctggtc acgcacccat aacagtgcat caatgactgt
    15601 attaccggta atgaagattc ggctatctgc aacgttttca cgcagcaagt tttgccggga
    15661 agtttcggtt ggagagaaat gatacatcgc cagatgcccg gtcaatgtac ggttagcctc
    15721 ttccggccac ggcgaataga gatcgcccgt gcgtaaacca gcctcaacgt gaccaacagg
    15781 aatacgctga taaaacgccg ccaggctggt tgccagcgtc gtcgttgtgt cgccgtgaac
    15841 cagcacgacg tctggtttga actcggcaag aataggtttt agcccttcca gaatccgaca
    15901 ggttatctct gtcaggccct gtcctggctg cattatgttg agatcgtagt caggtacaat
    15961 ggaaaagagt ttcagcacct gatcgagcat ctcccgatgc tgcgcagtga cgcaaacttt
    16021 agcctcaaaa aaaggatctt ttgccaacgc atgcaccaac ggagccatct tgatggcttc
    16081 cgggcgcgta ccaaatacag tcagtacttt cacatcgatt ctcttcgaat aagcggcgag
    16141 cgcctttgcg ctcaccgcag cagtgttgct atttcgagca acggcgggtt aatgcgacac
    16201 cagccccgat cagccccccg acaatgcccc acataatcat caggaaggca cgacgtgggc
    16261 tatcgcgttt taccggttct tccggcgtac gcaaatagcg ataggtctga aaacgcggat
    16321 ccagggttgg accaacattc agggtgttta acatggcccg attctgatca tagtcgagat
    16381 caaaggccgg accgacggcc tgtaaatttt ccagtcgagc ctggagcatt ggacgcccaa
    16441 gcaggaacat ttctgaatca ggtaattcct cggcaggcac atctgtcgca ctgcgcgaaa
    16501 tattatgctg ctcagcaatt ttcagcgcct gctcaatgct gttcatccgg cggtcgtaga
    16561 tggctttcgc cacctcttcc tgacgcttca cctgtgcttt catctggatg gtacgcgccg
    16621 cccatgcgcc tttcagctca tcattcagat ggctggctgc acgctggctg gcaaaagcaa
    16681 catactgacg taacaggtta ttagcgtcag gcgcggtttc ggcaataagc ttcacgctgt
    16741 cattgaccgc gcgggtaaag tctccgggga taaactggat gttgttaatc atttcatcca
    16801 gcaacgccgc atcggctttg ctgttgccca ccatccgctg cttgtaatag tcggtttgca
    16861 gccagaactc tctgcgggta tcccacgagg ccagttgcat aacaaactct ttataggctt
    16921 cgtccatgac cgatggttgg tcggcagaag ccatgtttga acggacatcc aggttacgca
    16981 aaaattgctg ctgcgagtaa tatcccccca gcatattcac cgttggacga tcggtaatcg
    17041 ccgtcgagct ccactcctga cgagcaaaaa acgtatacgc cagcgcgatt aacgcaaacg
    17101 ccagccccat gccaataatc catagcttcc cagcccacaa ggtacgaaac aacccacgaa
    17161 tatccagttc attttcagcg tcttcggccg gtttcccagg cattggttgt gtcatcacat
    17221 cctcatttat ttggttaaat tggggctgcc accacgattt ctacgcagtc tgcgttttac
    17281 gcgcttaata aagcgagcaa ctttccaggc acgcttaatg caatatccat agaggaggaa
    17341 tgctagcaaa aagagcacca acatgaccca ctccgggaca aaatgagaat attctgccag
    17401 cacgccaatg gaagcgagca gtgctgcggc aagggtaatc agcacaaacg cctggcggga
    17461 agtaaacccg gcacgcatga tcaaatggtg aatatgctga cggtcaggag agaatgggct
    17521 catgccttta cgcaggcgac ggtacataat cgccaccata tccattagcg gaatggcgat
    17581 tatccacaaa gcggtaaccg ggctgatggg atgggttttg ccttgggtcg tttcgagcag
    17641 gatccagatc acggtaaaac caatcagcgt actgcccgca tcacccataa agactttgta
    17701 gcggcgaccc aggataccaa ggttaagcat gatatatggc aggatggcgg cgatcatcgc
    17761 aaagcaccag attgcgaggc tggtttgccc gtcgaaccac aaaatcatac cgattgctgc
    17821 aaacgagacg caggacaacc cgcccagcaa gccatcaatg ccatcaacca tgttgaacgc
    17881 attaatggcc gcccagacgg caaatagcgt caggaagtaa ccaaacggtc cgagcaccat
    17941 ctcccaggag ccaaagatat aacccaggct actgagataa aggttgccaa acaccatcat
    18001 aacaatgcca acagcggcct gtatggtggc acggattttt acgctgatat caaaacggtc
    18061 atccagcgcg ccaatgaaaa caagcacacc ggcacaagcg agatagagag atgcatgcgg
    18121 aatatagtaa tcgacaattc cgaacgtgaa gcaaatccct gcgtaaaccg aaataccccc
    18181 aacgagtggt atcaacccct ggtgacgttt gcggaagttt ggtttatcca ctaaaccgac
    18241 tttttttgcc accttacggg caaaaaacag aaacagtgtc gtgaataaaa aaatactgat
    18301 gagatcagta ctcactgtca gtaaattcac aatgcatgct ctcagagaaa attattagca
    18361 gaagtataac cacgaagacc tttattcaga agggaaagtc cgttccgaac ccaccagtcc
    18421 tgtgcaaaaa ttaaccattt gatatgcaaa ttgcataaca tgccagcaaa ttccaggaat
    18481 tttcctaaca tcgccagcgc ggcattaaga ttacagatat aaaagcaaaa cgccacgtaa
    18541 acacgtggcg tttttggcat aagacaaatt tatgagcgtt tcatcatttc gaagaaatcg
    18601 tcattggtct tggtcattgc cagtttatta atgaggaatt ccattgcatc gatttcgccc
    18661 atcgggtgaa tgattttgcg caggatccac attttctgca gttcttcctg agtcgtgagc
    18721 agctcttctt tacgggtacc ggaacggttg tagtcgatag ccgggaagac gcgtttttca
    18781 gcgatcttac gagagaggtg cagttccatg ttgcctgtac ctttaaactc ttcgtaaata
    18841 acttcgtcca ttttagaacc ggtatcgata agcgccgtcg cgataatggt caggctgccg
    18901 ccctcttcca cgttacgcgc cgcaccgaag aagcgtttcg gacgatgcag ggcgttggca
    18961 tccacaccac cggtcaacac tttacctgac gccggaacaa cggtgttgta agcgcgcgcc
    19021 agacgagtga tggagtcgag cagaatgata acgtctttct tgtgctcaac caggcgtttc
    19081 gccttctcga tcaccatttc cgcaacctga acgtggcgag atgcgggttc gtcaaaggta
    19141 gaagcaacaa cttcaccttt taccagtcgc tgcatctcgg ttacttcttc cggacgttcg
    19201 tcgatcagca gaaccatcag cacacaatcc gggtggttgt aagcaatgct ctgagcaatg
    19261 ttctgcagca gcatggtttt accggctttc ggcggtgcca caatcagacc acgctgacca
    19321 cgaccgatag gtgatgccag atccagtacg cgagcggtta aatcttcagt agaaccgtta
    19381 ccacgttcca tacgcagacg agagtttgcg tgcagcgggg ttaagttctc aaagaggatt
    19441 ttgttgcggg cgttttcagg tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa
    19501 tagcgttcac cttctttcgg cgggcgaatc ttaccagaga tggtatcacc agtgcggagg
    19561 ttgaaacggc ggatttggct aggggaaacg tagatgtcat caggaccggc gaggtaggag
    19621 ctgtctgcgg aacggaggaa accaaatcca tcctgcaata tctccagtac gccatcacca
    19681 aagatatctt cgccactctt tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta
    19741 cgcatacgag ccaggttttc cagccccata ttttcgccga gagtgatcag ctcagaaacc
    19801 ggcgtattct ttaattcggt aagattcata gtggtgtgag ttcttaaact tggggtaaat
    19861 ctcgaactta atgttgtgaa tggtatggca ggatcatcca tgcctgttta cggtcatcga
    19921 ctcatgtctt ttcgctgcct ggtcacagga gagtacgcag aactgaaacg acaagacgga
    19981 ttgagtgacg agccagaaat ctgttcactt cgcatttaag ataaaacggg aagcgttgga
    20041 aattacaaga ttcaaactta ataaggtatg tttaatacga agtcaacact aagttagcat
    20101 gactcacgcc gggcgtccag tttttagcga cggggcaccc gaacatgaaa ttcccttacg
    20161 ccaggttagc gtcgaggaac tctttcaact gacctttaga cagtgcgccc actttggttg
    20221 ccgccacttc accgtttttg aacagcagca gagtcgggat accacggatg ccatatttcg
    20281 gcgcagtgcc agggttttga tcgatgttca gttttgcaac ggtcagtttg ccctgatatt
    20341 cgtcagcgat ttcatccaga atcggggcga tcattttgca cggaccgcac cactctgccc
    20401 agaaatcgac gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag
    20461 tcaggtgaat aattttatcg ctcatatata actccacagg aataagcctg gcgtgttggt
    20521 ttcgttgttg gtgtaacatt aaccaactaa aggttgactt tatttcaccg gatacgcttt
    20581 cgtaaagcaa tagtaagctg atattctacc acactatgag caaaacacat ttaacagaac
    20641 agaagttttc cgacttcgcc ctgcatccga aggttgtaga agcccttgaa aaaaaagggt
    20701 ttcataactg tacgcccatt caggcactgg cccttccgct gacgctggcg ggtcgtgacg
    20761 tagccgggca ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg tcaacgtttc
    20821 attgtcttct ctctcatccc gcgattgccg atcgcaaggt gaatcagccg cgtgccttaa
    20881 ttatggcacc gacgcgtgaa cttgccgtgc agatccatgc cgatgcggaa ccgctggcgg
    20941 aagctaccgg cctgaagctg ggtctggctt acggtggtga tggttacgac aaacagctga
    21001 aagtgctgga aagcggcgtt gacattctga ttggcaccac ggggcgttta attgactacg
    21061 ccaagcagaa ccacattaac ctcggtgcca ttcaggtagt ggtactggac gaagccgatc
    21121 gcatgtacga tctgggcttt attaaagata tccgctggct gttccgccgt atgccgcctg
    21181 caaaccagcg cctcaacatg ctgttctccg ccacgctttc gtaccgggta cgtgaactgg
    21241 cgttcgagca gatgaacaat gccgaatata ttgaagtgga accggaacag aaaacgggcc
    21301 accgtattaa agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa
    21361 cgctgatcga agaagagtgg ccagacagag cgattatttt cgccaatacc aaacaccgtt
    21421 gtgaagagat ctggggccac ctggcggcag atggtcatcg tgtcggtttg ttgacaggcg
    21481 atgtcgcgca gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggc gatctggata
    21541 ttctggttgc caccgacgtt gccgcgcgtg gtttgcatat tccggcagtg acgcacgtct
    21601 ttaactacga tttacccgat gactgtgaag attacgttca ccgtattggt cgtacaggtc
    21661 gcgcaggcgc aagcggtcac tctattagcc tggcgtgtga agagtatgca ttgaatttgc
    21721 ctgctattga gacctatatt ggtcactcaa ttccggtaag caaatacaat ccggacgcat
    21781 tgatgaccga tctgccaaaa ccgctgcgcc tcacgcgccc gcgcacaggc aatggtccgc
    21841 gtcgtactgg cgctccgcgt aatcgtcgtc gttcaggtta aataaaaaat gtcatactca
    21901 aaatcattca aactgtatca agatggcaag agaatgaatc ccgatgagct tactgtagta
    21961 agtgattcag gtgaaagaat gcagccaaca cagagacaga ttgaaggatg aagagtatgg
    22021 gttccacctc atcgctgtat gcagccattg atctcggttc gaatagtttt catatgctgg
    22081 ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc
    22141 tggctgctgg cctgaatagc gaaaatgccc tctccaatga agcaatggag cgcggttggc
    22201 aatgtttgcg cctgtttgct gaacgtctgc aagatattcc tccctcgcaa attcgcgttg
    22261 tcgctacggc gacgttacgc cttgccgtca atgcgggtga ttttattgcc aaagcgcagg
    22321 aaatcctcgg ttgtccggta caggtgatca gcggtgaaga ggaagcacgt ctgatttatc
    22381 agggtgttgc tcacaccact ggcggtgccg atcagcgcct ggtagtggat atcggcggtg
    22441 ccagtactga actggtaacc ggcacgggtg cacaaaccac ctcgttgttc agcctgtcga
    22501 tgggctgcgt cacctggctg gaacgctatt ttgccgatcg taatctgggg caggaaaatt
    22561 ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat gaattacggt
    22621 atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt gcaggcgtta caggaaatca
    22681 tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa ttgaaacagc
    22741 gagccattca ttgcggtcgg ctggaggaac tggagattga cgggctgacg ctggaacgtg
    22801 cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat ttttaccgaa ctgaatattc
    22861 agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggc atgttgcatc
    22921 tggccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcaacgc cgctttatga
    22981 tcgatattga tcaggcacag cgcgtagcca aagttgcggc taacttcttc gatcaggtgg
    23041 aaaacgaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc tgtcagcttc
    23101 atgaaatcgg cctgagcgtt gacttcaaac aagcgccgca acacgctgct tatctggtgc
    23161 gtaatctgga tcttcccggt tttacccccg cacagaaaaa attgctggcg acgctactgc
    23221 tcaaccagac taatccggtc gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc
    23281 gcgtcgcaga acaactctgc cgtttactac gcctggccat catttttgcc agccgtcgcc
    23341 gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg accttgacgc
    23401 ttccgcaagg ttggctaacc caacatccgc tgggtaaaga gattattgct caggaaagcc
    23461 agtggcagag ctatgtccac tggccgctgg aagtgcatta atgagtaagt gccggatgcg
    23521 atgctgacgc atcttttccg gccttgatta tttccctcgt tttgccgcca tcatcgcttt
    23581 cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga ccactttgcg
    23641 ctcctgttcc caaatcagat catcctgcgg cagctccagc aaaaagcggc tcggctccgg
    23701 gcgcaccagt tcgccgtact gacggcgttc tttacacagc gtaaaggtca attccttctg
    23761 ggcgcgggta atgccgacat aggccagccg ccgctcctca tcgatattat cttcatcgat
    23821 actgctctgg tgcggcaaaa acccttcttc cataccgacc atgtagacat aaggaaactc
    23881 cagtcctttt gacgcgtgga gagtcatcag ttgcacctga tccagctctt cttcactctc
    23941 accacgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg
    24001 ctcatccagt tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt
    24061 cttcatgcgc atttcggcgg ctttcgggct gggcgatgtt tcgtacagcc aggattcata
    24121 atccatgcca tggatcagat cacgcaccgc agcaatcggc tcccgctccg ccaggcgctg
    24181 gatttctgcc aaccagtgag tgaagcgggt caatgcttca taaccacgtc cgctaagcgt
    24241 ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg tcatcgccca
    24301 ctcacccagc tttttcagcg tagccgggcc aatctctcgc ttcggcgtgt taacgatacg
    24361 cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc agataagcca gcaagtcctt
    24421 gatttcagga cgagagaaaa acgacgtacc accggatatt ttgtacggga tgcggttttg
    24481 catcaggaat ttttcaaata cccgcgactg atggttgccg cgataaagaa tggcgtaatc
    24541 tttgtactgc gttttattga cgaagtgatg ggcgatcagc tcgccagtaa cgcgctcggc
    24601 ttcatgttcc tcgttattcg cgcttaaaac ttttagctct gtgccgtaac ccagttcgga
    24661 gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc aggatgttcg ccgctttcag
    24721 aatacgcccg gaagagcgat agttctgctc aagcttaatc accttcagcg ccggaaaatc
    24781 ctgactcagc agcaccaggt tttgcggacg tgcaccgcgc caggagtaga tcgactggtc
    24841 atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc agcagtttca ccagttcata
    24901 ctggctggtg ttggtgtcct gatactcatc caccagcagg tagcgaattt tgttctgcca
    24961 gcgttcgcgt acttcttcat tgcgttgcag cagcaacgtc ggcaataaaa tcaggtcatc
    25021 gaagtcgaga acgttacagg ctttcaggtg tgcatcatac agcccataac aatgggcaaa
    25081 aatacgatcc cgctcgccct ttgcctctgc cgccgcctgc gccggagttt tgagatcgtt
    25141 cttccagtta gagatggtcg aaatcagttg ttgcaggaga actttgtcat cttcaatcag
    25201 cccctcggtc aactctttaa gcaaagcaag ctgatcggta tcgtcaaata acgagaagtt
    25261 cgctttcatc ccaagcgccg catactcgcg tttgatgata tccagcccca acgtatggaa
    25321 agtggagatc atcagcccac gcgcctcttt acgccccagc gtctgcccta cacgctcttt
    25381 catctcgcgc gctgctttat tagtaaaggt caccgccgca atgtgccgcg cctgataacc
    25441 gcaaccgcgg atcagatggg cgattttatt ggtgataaca cgagttttac cggaacccgc
    25501 gcccgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt ggccggggtt
    25561 tagacgcata ggtgtattgc tcaatcttcg aacgggggga ggattgtagc agaatcgcat
    25621 ttgaatgtga ccgcgtgtaa gccgctaacc ggttggctgc ggatgtttaa atcagagaga
    25681 gaagaccgaa atcggagttc atcgtgaatc cattagactt agaaaatatc gggtcgtaac
    25741 gttgtgttat tactttttca gagtttacta aggagaggtt tactcgtggc gattaatttc
    25801 agccctaagg ttggtgaaat actggaatgc aactttggta actatcctgt tagccaaaat
    25861 ggaccgttca gcacgacgta ttacgatggc cgcattcctc ctgaaatgat aaaaaacagg
    25921 ttagttgtcg tgctcaacgg aaaaattaat ggcaacgcat tcatcgtcgt tccattatca
    25981 accactcgcg accatgacaa gttaaaacgt ggaatgcatg ttgaaatagc cagtaatgtt
    26041 atcaatgacc tgcaattctt tgatcagcag attcgttggg ccaaaaccga cctggtacaa
    26101 caagtcagtc gaaacagatt aaacagagca agaacttaca gagggtatct taatcaatgt
    26161 ttgccacatg agttggttgc agacatacag agggcggtca tcaagtcaat caatgccata
    26221 tcgctcataa attgacaata aaatcaccat ccccgataat gactttgtag cccatagagg
    26281 ccctaagttt tctccccttg agggacctgc gaagcccgga ttttccgggc ttttgccata
    26341 tatgtaccag cccctccctg actgacaatt acgttagcat cggcggatca tgctaaaatc
    26401 gccgcctgac aattattcac tcaaggcacg atcatggcaa aaacagcagc agcactgcat
    26461 atccttgtaa aagaagagaa actggctctg gatcttctcg agcagattaa gaacggggcc
    26521 gattttggca agctggcgaa gaaacactcc atttgcccat caggcaaacg cggcggtgat
    26581 ttaggtgaat tccgccaggg tcagatggtt ccggcgttcg ataaagttgt gttctcttgt
    26641 ccggtactgg agccaaccgg cccgctgcac acccagttcg gttaccacat cattaaggtg
    26701 ctgtaccgta actaatagca aggccttctc cgggagaagg ccttgaactc tgaatcgcta
    26761 ttaacccgca acagcaatac gtttcatatc agtcatgtag ccgcgcagtt tcttacctac
    26821 ctgctcaatc gcatggctgc gaatcgcttc gttcaaatca cgcagttgcg cgttatctac
    26881 cgcaccttcc ggaatagctt tacccaaatc gcccggttgc agctctgcca taaacggttt
    26941 cagcaacggc acacaagcgt aagagaacag atagttaccg tactcagcgg tatcagagat
    27001 aaccacgttc atttcgtaca gacgcttacg ggcgatggtg ttggcaatca gcggcagttc
    27061 gtgcagtgat tcatagtaag cagattcttc gatgatgccg gaatcaacca tggtttcaaa
    27121 cgccagctca acgcccgctt tcaccatcgc gatcatcagt acgcctttat cgaagtactc
    27181 ctgctcgccg attttgcctt catactgcgg cgcggtttca aacgcggttt tgccggtctc
    27241 ttcacgccag gtcagcagtt tcttatcatc gttggcccag tccgccatca tgccggagga
    27301 gaattcgccg gagatgatgt cgtccatatg tttctggaac agcggtgcca tgatctcttt
    27361 cagctgttct gacaacgcgt aagcgcgcag tttcgccggg ttagagagac ggtccatcat
    27421 cagggtgatg ccgccctgtt tcagcgcttc ggtgatggtt tcccagccga actgaatcag
    27481 tttttctgcg tatgccggat cggtgccttc ttccaccagc ttgtcgaagc acagcagaga
    27541 gccggcctgc aacataccgc acagaatggt ttgctcgccc atcaggtcag atttcacttc
    27601 cgcaacgaaa gaggattcca gcacgcccgc acgatgacca ccggttgccg ccgcccatgc
    27661 tttagcgatc gccatgcctt cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt
    27721 cggtacgccg aatccacgtt tgtactcttc acgtacttcg gtgcctgggc atttcggcgc
    27781 aaccattact acggtgatgt ctttacggat ctgctcgccc acttcgacga tgttgaaacc
    27841 atgcgagtaa cccagcgccg cgccgtcttt catcagtggc tgtacggtgt gcactacatc
    27901 agagtgctgc ttgtccggcg tcaggttaac caccagatcc gcctgtggga tcagttcttc
    27961 gtaagtaccc actttaaaac cattttcggt cgctttacgc caggacgcgc gcttctcggc
    28021 aatcgcttct ttacgcagag cgtaggagat atcgagacca gaatcacgca tgttcaggcc
    28081 ctggttcaga ccctgtgcgc cacagccgac gatgaccact tttttaccct gaaggtagct
    28141 cgcgccatcg gcgaattcgt cgcggcccat aaagcgacat ttgcccaact gtgccagttg
    28201 ctggcgcagg ttcagtgtat tgaagtagtt agccatggtg attcctcgtg atgttgtgct
    28261 tcttattgtt cggttcgcgt ttgcaagatt gaattcacta tatgacagga aatttattgc
    28321 ggaaattgat atattcacaa catcacattg caatttttgc aacgtcaaca tcgagggctg
    28381 tccctgtgga tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc cgccattttg
    28441 gccgcagcgc gcgggcgatg cacgttagcc catccacgct ctcacggcag attcagcgcc
    28501 tggaagaaga tctcggtcag ccgctgtttg tgcgcgataa ccgcacggtg acgctgactg
    28561 aagcgggcga agaactgcgc gttttcgccc agcaaacgct gttgcagtat cagcagctgc
    28621 gccacaccat cgatcagcaa gggccgtcgc tctctggcga attacatatc ttctgctcgg
    28681 tgaccgctgc ctacagccat ctgccgccga ttctggatcg cttccgcgcg gaacacccgt
    28741 cggtggagat taaactcact actggtgatg cggcagatgc aatggaaaaa gtggtcacgg
    28801 gagaagcgga tctggcgatt gccgggaaac cggagacgct acccggcgct gtagcgtttt
    28861 cgatgctgga gaatctggcg gtagtgctga ttgcgcccgc gctgccctgc ccggtgcgta
    28921 atcaggtctc ggtagagaag ccggactggt caacggtacc gtttatcatg gccgatcagg
    28981 ggccagtacg ccgccgcatt gaactgtggt ttcgacgcaa taaaatcagc aacccaatga
    29041 tttacgccac ggttggcggg catgaagcga tggtatcgat ggtggcgctc ggctgtggtg
    29101 tggcgctgtt gccggaagtg gtgctggaaa acagcccgga gcctgtacgc aaccgggtaa
    29161 tgattttaga tcgcagcgat gagaaaacgc cgtttgaact gggcgtgtgt gcacaaaaaa
    29221 agcggctgca tgagccgctg attgaggcgt tctggaggat tttgccgaac cacaaatgac
    29281 gttgtcgcgc gagtaggcct gataagcgaa gcgctatcag gcatttttcc ctaacccgcc
    29341 aaaaagaacc tgaacgccgg gttattggtt tcgtcgtggc aatcgtagcc cagctcattc
    29401 agccgggttt cgaaatccgg ttcatggtcg ccaagttcga acgccgccag tacgcgcccg
    29461 tagtcggtgc catggctgcg atagtggaac aaagaaatgt tccagtgcgt acccagcgtg
    29521 ttgaggaagc gcagcagcgc gcccggtgat tccgggaatt cgaagctgta gaggcgttcc
    29581 tgcaacggat gtgacggacg cccgccgacc atatagcgca catgcaactt cgccatttcg
    29641 tcgtcggaga gatccaccac gctgtagccg ccgtcgttga gcatctgcaa aatttctttg
    29701 cgctcttcga ggccgcggct caggcgcacg ccgacaaaga tgcaggcgtt tttggcatcg
    29761 gcaaaacggt agttgaactc ggtgaccgat cgcccgccaa gcagttggca gaatttgagg
    29821 aagctgcctt tttcttccgg aatggtcacc gccaacaacg cttcacgctg ttcgcccagt
    29881 tcgcagcgtt ccgagacgta gcgcaggccg tggaagttca cgttggcacc ggaaagaata
    29941 tgcgccagcc gttcgccgcg aatgttgtgc tgggcgatat attttttcat tcccgccagc
    30001 gccagcgcgc cagagggttc cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc
    30061 gcacagatcg catcactatc gacggtgatg atatcgtcga gatactcctg gcacaaacgg
    30121 aaggtttcgt cgccgatgcg ttttaccgca acgccttcag caaacagccc cacgcgcggc
    30181 agatcaaccg gatgacccgc atccagcgct gctttcaggc aggcggagtc ttccgcttct
    30241 acggcgatta ctttgatttg cggcatcagt tgtttgatca gcaccgccac gccagcagcc
    30301 agaccgccgc cgccgactgg cacaaatacg cggtcgagat gggcgtcctg ctggagcagt
    30361 tccagcgcca gcgtgccttg cccggcaatc accatcggat ggtcgaacgg cggcacccag
    30421 gtgaacccct gctgctgtga cagttcgatc gctttggctt tcgcttcatc aaagttcgcg
    30481 ccgtggagca gcacttcgcc gccgaagccg cgcaccgcgt cgactttgat gtcggcggtg
    30541 gcggttggca taacgatcag ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc
    30601 tgcgcgtggt tacccgcaga agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg
    30661 cccgccatca tggcgtatgc gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg
    30721 cgcttcacca gaatcacgtt atcaagacga gacgagagct tttccatttt ttgtagcggc
    30781 gtgacctgcg ccgcttcgta gaccggcgcg cgcagcaccg ctcttaaata ttcagcacct
    30841 tccggggcac cggacagggg ttgcgagtca gccattatta accccccagt ttcgatttat
    30901 cgcgcaccgc gcctttgtcg gcgctggttg ccaggctggc ataagcacgc agggcaaagg
    30961 agatctgacg ttcacgattt ttcggcgtcc aggctttgtc accccgagct tcctgcgctt
    31021 cacgacgcgc cgccagttcg gcatcgctta cctgtaactg aatgccacgg ttcggaatgt
    31081 cgatggcgat caggtcgccg tcttcaatca ggccaatgct gccgccgctt gccgcttccg
    31141 gtgagacgtg gccgatagaa aggccagagg tgccgccaga gaaacgaccg tcggtgatca
    31201 gcgcacaggc tttaccgagc cccattgatt tcaggaagct ggttgggtag agcatttcct
    31261 gcatccccgg accacctttt gggccttcat agcgaattac taccacatct ccggcgacaa
    31321 ctttaccgcc gagaatcgct tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc
    31381 cggtgaattt gaggatgctg tcatcgacgc ccgcagtttt aacgatgcag ccgttttccg
    31441 cgaaattacc gtagagcacc gccaggccgc cgtctttgct ataggcgtgt tccagcgagc
    31501 ggatacagcc attggcgcga tcgtcgtcca gcgtatccca acggcaatct tgcgagaatg
    31561 cctgggtggt acggataccc gcagggccgg cgcggaacat attttttacc gcgtcatcct
    31621 gggtcagcat aacgtcgtat tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt
    31681 tcacatcacg gttcagtaac cccgcacgat ccagctcgcc gagaataccg ataacaccac
    31741 cagcacggtg aacatcttcc atatggtatt tttgggtgct cggcgcgact ttgcataact
    31801 gcggaacctt gcgggaaagc ttatcgatat cactcatggt gaagtcgatt tccgcttcct
    31861 gcgccgccgc cagcaggtga agtacggtgt tagtcgatcc acccatcgcg atatccagcg
    31921 tcatggcatt ttcaaacgcc gctttgctag caatattgcg gggcagtgca ctttcgtcgt
    31981 tttgctcgta gtaacgtttg gtcaattcaa caatgcgttt accagcattg aggaacagct
    32041 gcttacggtc agagtgggtt gccagcagcg agccgttgcc cggctgcgac agacccagcg
    32101 cttcggtcag gcagttcatt gagttagcgg taaacatccc ggagcaggaa ccgcaggttg
    32161 ggcatgcgga acgttcaacc tgatcgctct gggagtcaga tactttcggg tctgcgccct
    32221 ggatcatcgc atcaaccaga tcgagcttga tgatctgatc ggacagtttg gttttcccgg
    32281 cctccatcgg gccgccggaa acaaagatca ccggaatatt cagacgcagg gaagccatca
    32341 gcatccccgg ggtgattttg tcacagttag agatgcagac catggcgtcg gcgcagtggg
    32401 cgttgaccat atactcaacg gaatcagcga tcagttcgcg agatggcagt gaataaagca
    32461 tccccccgtg gcccatggca atcccatcat ccaccgcaat ggtgttgaac tctttggcaa
    32521 cgccgccagc cgcttcaatt tgttcggcga ccagtttacc gagatcgcgc agatggacgt
    32581 gacccggtac aaattgggtg aacgagttca caaccgcgat aatcggctta ccgaaatcgg
    32641 cgtcggtcat tccggtggcg cgccacagcg cacgagcacc cgccatatta cgaccatgag
    32701 tggtggtggc ggaacggtac ttaggcatac tttatttact cccagtgtct gtctcgtaaa
    32761 tgggacggtg cgtgccgtcc cattttttgt atttattgat taacttgatc taaccagccc
    32821 catttatctt cggtttcgcc ggtaaagagg ccgaagaagg cttgctgaat gcgtttggtg
    32881 accgggccac aacggccttc gccaacctga ataccgtcta cactgcgcac tggcgtgatt
    32941 tctgccgccg taccggacat aaagacttca tccgccaggt acaaggattc gcgcgacagc
    33001 acctgctcac gcacttcaat tcccagctct ttcgccagtt tgatgatggc atcacgggta
    33061 atacccggca gcgcggagga ggtgaacggt ggggtgaaca gcacaccatc tttcacttca
    33121 aacaggtttt cgcctgcgcc ttcagagata taaccgttca catccagcgc gataccttcc
    33181 tgataaccgt ggcggcgcgc ttcgctaccc accagcaggg aagagagata gttaccaccg
    33241 gcttttgccg cagttgggat ggtgtttggt gctgcgcggt tccaggagga aaccatcgca
    33301 tcgatcccct gctccagcgc ttctgcgccc agatacgctc cccacgggaa agcggcgata
    33361 atcacatcgg ttgagtatcc cgctggcggg tttacgccca tgccaacatc accaacgaag
    33421 atcagcggac ggatataggc gctggtgaga ttgtttttgc ggatcacgtc acggcaagct
    33481 tccatcagct catcaatgct ctgcgaaacc gggaagcgat agattttggc ggagtcatgc
    33541 agacgctgca tatgctcacg atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa
    33601 cggatgcctt caaaaaccga ggtgccatag tgcagcgcgt gcgacatcac atgcaccttc
    33661 gcgtcttccc agcgaaccat ctccccattg aaccaaatgt aatcagcttt cttcgtggtc
    33721 atttttatat tccttttgcg ctcaggcgcg gatttgttgt gatgtggttg tgctctggca
    33781 gatggcaacg tgtgcgacgt ccaccagttt atttaactga ctaaacagta agtcgaccga
    33841 ccgtgggctg gcaacggtca attcgatatt tatattttgt gcatcgctgg cggcggccat
    33901 attcattgag cagacgtgga aaccacgatg acgcaccacg cgtaaaacac gttctaaggt
    33961 ttccggattg aagcgagccg atacattgac ctgatgttgc atcatgataa tttctccaac
    34021 atttctgaat tactggcacc aggcggcacc agcggccaga cgttctcaag ttcgtcgatt
    34081 gagacatgaa gcaggtatgg cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc
    34141 tggtctttac gggtgatgtg ttggccaggg atgccgaagg cgctggctaa cttgaggaaa
    34201 tcggggttat cggtaagggt ggtttcgctg tatcgttcct ggaaaaacag ttgctgccat
    34261 tgtcgaacca tccctaaccg ttggttatcg agtaagacga ttttcaacgg taactgcttg
    34321 cgttttacgg tgcccagctc ttgtacattc atcatgaaag agccgtcacc ggagatacag
    34381 acgacggtat cgtttggtcg cgcgacttgc gcgccaaccg ccgccggtaa accaaaaccc
    34441 atggtgccta agccgctgga ggtaatgaaa ttttccgggc gagtgtgtgc gatgtgctgc
    34501 gcggcccaca tctgatgctg ccccacatct gtggtcacaa cgcaatccgc aggtttacga
    34561 tccgacagtt gttttaacaa caacggcgcg tagatagcgt caccgggatg gtcgtaacgc
    34621 caggcatgtt catcacgcag ctgcgcgcag tattgctgcc agtcattgat atttaatggc
    34681 tgctgtaatg ctggtaacag cgcatttaaa tcaccctgta atgccacatg tgcctgacgc
    34741 agcttgttca tttctgccgg gtcgatatcc atatggataa cactggcgtg tggtgcgaag
    34801 gtgttcaatt tgccggtcac ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg
    34861 cactcctgca ccgcgaagtt cgccgctttg gtgccatgca ttcccagcat gcccagatag
    34921 tacggataat ctgcttcaac tgcgcccagc cctttcagcg tgcaggtggc aggcattttt
    34981 gtggtagcga gaaattctcg taaagcagga actgcctgcg ccatacccac gccaccacca
    35041 acgtacagca tcggtttttg cgcttttgcc agcatctggc gcgcttgctc aacttcggca
    35101 tgtgggaaag tcacttcgtt ttcaacggtg gtgaaccacg gttccaggtc gccgctggct
    35161 aactggatat cttttgggat atcgaccaga accggaccag gacgacctga gctggcaacg
    35221 tcgaatgctt cagccataat gcgcggcaac tcttccagcg actgcaccag aaagctgtgc
    35281 ttggtacagg ctaacgacaa tcccaggaca tccacttcct gaaatgcgtc agtaccgata
    35341 aacggtgcgg acacttgacc ggtgatggca acaacaggga tagaatctaa cagtgcgtcc
    35401 gcaagcccgg ttatcaggtt ggttgcgccc ggaccagacg tggcgataca tacgccagtt
    35461 ttgccggtag cacgggcata accgatagcc gccattgccg caccctgctc atgtcggcac
    35521 agcaagtgct ccacgccgcc gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc
    35581 ggataaccga aaacggtgtt cacaccctgt gcccgcaacg catgtaccac ccactgtgcg
    35641 ccattcatag ttagttcccc gtcctgaatc ttgggaaaca gaattttgtg ctgttattca
    35701 ttgtctgctc ctcggttatg tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg
    35761 gggtcttagt tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca
    35821 cggtgggata ataatcacca ccacgctaat cacgaccagg ctaatcactc gtagaagggc
    35881 tgtcattttg tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accatagatt
    35941 ttgcgaatag cacaagatat tttttggccg ttttttatga tggctttaat aatttcaata
    36001 gaaattgttg ttttttaaaa agaaaatagt gagtgaaaaa aattcaattt cagtacgaat
    36061 gttgttccct ccctcgtagt tcatcctttg tccgctgcat ttttgcgagc atcattccac
    36121 cgccagaaca actcaacttc acttgcacaa ataaacagga atcagccact aaaattcacg
    36181 aattagcggg ctacagcagc gatatcttat gcacataatg gtgactcaag gagggcttat
    36241 gtcactgtca attgttcata cccgcgcagc cctgggagta aatgcgcccc caatcactgt
    36301 tgaggtacat atcagtaaag gtttgcccgg cttaacgatg gtgggcttac cagaaacaac
    36361 ggtaaaagaa gctcgcgatc gcgtgcgcag cgccattatc aatagcggat atgaatatcc
    36421 ggcgaaaaaa atcaccatta accttgctcc agccgatcta ccaaaagaag ggggacgata
    36481 tgatttacct atcgccattg cgttgctggc ggcctcagaa cagcttacag ccaataagtt
    36541 agatgaatat gaattagtcg gagaactggc gcttacaggc gctctgcgtg gcgttcccgg
    36601 tgcaatctcc agtgcaactg aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga
    36661 taacgaagat gaagtggggc taattaacgg tgaaggatgc ctggtagccg atcatctgca
    36721 agctgtctgt gcgtttctgg aaggtaagca cgctctcgaa cgcccgaaac caactgatgc
    36781 agtatcccgg gcgctacaac atgatctcag tgatgttgtc ggtcaggagc aaggaaagcg
    36841 aggactggaa attaccgccg ctggcgggca caacctttta ctgattgggc cgccgggaac
    36901 aggtaaaaca atgctcgcca gccgtattaa tggtcttttg ccagatttaa gcaatgaaga
    36961 ggcactggag agcgctgcga tattaagtct ggtaaatgct gaatcagtac aaaaacaatg
    37021 gcggcagcgc ccgttccgct cacctcatca cagtgcgtcg ttaacggcga tggtgggcgg
    37081 tggtgcaatt ccagggccag gtgaaatttc gttggcgcat aacggcgtgc tttttcttga
    37141 tgagctacct gaatttgaac ggcgtacact ggatgctttg cgagagccga ttgaatccgg
    37201 gcagatccat ctttcacgca cacgagcaaa aataacctat ccagcccgtt tccagcttgt
    37261 tgcggcgatg aatcccagcc ctaccggaca ttatcaggga aaccataacc gctgcacgcc
    37321 agaacagaca ttacgttatc tcaaccggct ctcggggccc tttctcgacc gcttcgatct
    37381 ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag tgccgggaga
    37441 aagcagcacc accgttaaac aacgtgtaat ggctgccaga gagcgccaat ttaagcggca
    37501 gaataagttg aacgcctggc tggatagtcc ggaaatacgc aaattctgca agcttgagag
    37561 cgaagatgcg cagtggctgg aagaaacgct gatccatctg gggttatcga ttcgtgcctg
    37621 gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg acattatcac
    37681 acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc tcatccatct
    37741 gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatcg tcagaatcgg
    37801 tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga aaacgttttg
    37861 gacgcttgat acgcgtcata tacttggacc acacgcgttc tgcttctgtc actggctcac
    37921 gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc
    37981 caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc tctttgatgg
    38041 tgaaatcacc atggcgagag aatccacgtg gataatgttt attgtcgaaa tatcgattag
    38101 tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag ctagttatgg
    38161 cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc ataacgacaa
    38221 ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg gaagttagcc
    38281 gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca gcagtgagct
    38341 ttcgaatcag acaactggaa aatcaactgg gtgtgaacct tttcacccgc cacagaaaca
    38401 atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca
    38461 cctggcaggc cgcccgtaag gaggtggcgc atacctcacg acataacgag ttttctatcg
    38521 gtgccagcgc ctcattgtgg gaatgtatgc ttaatcagtg gctgggacgt ttgtatcaaa
    38581 atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg
    38641 taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg cccaaaatgg
    38701 acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc agtgcccctt
    38761 ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat tttcaacagc
    38821 atgaggcagg tttgatcggt gctgacgaag tgcccattct gacaaccagt tctgctgaac
    38881 tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtc agctgggcgc
    38941 gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg ccgctttatg
    39001 ccatatggct gcaaaatagc gataaaaata cgttgattcg cgatctattg aaaattaacg
    39061 tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa ggatttactt
    39121 cagagagggt tatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggca
    39181 ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc taccaattga
    39241 actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg
    39301 acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta caaccaccgg
    39361 ttttatgacc ggctt
//
LOCUS       DAZFTN010000034        39373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFTN010000034 DAZFTN010000000
VERSION     DAZFTN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            393..1652
                     /gene="codB"
                     /locus_tag="ACHKV2_001928"
     CDS             393..1652
                     /gene="codB"
                     /locus_tag="ACHKV2_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6939814.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1642..2925
                     /gene="codA"
                     /locus_tag="ACHKV2_001929"
     CDS             1642..2925
                     /gene="codA"
                     /locus_tag="ACHKV2_001929"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO6939815.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="ACHKV2_001930"
     CDS             complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="ACHKV2_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO6939816.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4067..4726
                     /gene="cynT"
                     /locus_tag="ACHKV2_001931"
     CDS             4067..4726
                     /gene="cynT"
                     /locus_tag="ACHKV2_001931"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO6939817.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4757..5227
                     /gene="cynS"
                     /locus_tag="ACHKV2_001932"
     CDS             4757..5227
                     /gene="cynS"
                     /locus_tag="ACHKV2_001932"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO6939818.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5233..6414
                     /gene="cynX"
                     /locus_tag="ACHKV2_001933"
     CDS             5233..6414
                     /gene="cynX"
                     /locus_tag="ACHKV2_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO6939819.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6517..7128)
                     /gene="lacA"
                     /locus_tag="ACHKV2_001934"
     CDS             complement(6517..7128)
                     /gene="lacA"
                     /locus_tag="ACHKV2_001934"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO6939820.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(7194..8447)
                     /gene="lacY"
                     /locus_tag="ACHKV2_001935"
     CDS             complement(7194..8447)
                     /gene="lacY"
                     /locus_tag="ACHKV2_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO6939821.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8499..11573)
                     /gene="lacZ"
                     /locus_tag="ACHKV2_001936"
     CDS             complement(8499..11573)
                     /gene="lacZ"
                     /locus_tag="ACHKV2_001936"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO6939822.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11696..12778)
                     /gene="lacI"
                     /locus_tag="ACHKV2_001937"
     CDS             complement(11696..12778)
                     /gene="lacI"
                     /locus_tag="ACHKV2_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO6939823.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(12815..13768)
                     /locus_tag="ACHKV2_001938"
     CDS             complement(12815..13768)
                     /locus_tag="ACHKV2_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO6939824.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(13788..14564)
                     /locus_tag="ACHKV2_001939"
     CDS             complement(13788..14564)
                     /locus_tag="ACHKV2_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO6939825.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14679..15512)
                     /gene="mhpR"
                     /locus_tag="ACHKV2_001940"
     CDS             complement(14679..15512)
                     /gene="mhpR"
                     /locus_tag="ACHKV2_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO6939826.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15703..17367
                     /gene="mhpA"
                     /locus_tag="ACHKV2_001941"
     CDS             15703..17367
                     /gene="mhpA"
                     /locus_tag="ACHKV2_001941"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO6939827.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            17369..18313
                     /gene="mhpB"
                     /locus_tag="ACHKV2_001942"
     CDS             17369..18313
                     /gene="mhpB"
                     /locus_tag="ACHKV2_001942"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO6939828.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            18331..19197
                     /gene="mhpC"
                     /locus_tag="ACHKV2_001943"
     CDS             18331..19197
                     /gene="mhpC"
                     /locus_tag="ACHKV2_001943"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO6939829.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            19207..20016
                     /gene="mhpD"
                     /locus_tag="ACHKV2_001944"
     CDS             19207..20016
                     /gene="mhpD"
                     /locus_tag="ACHKV2_001944"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO6939830.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            20013..20963
                     /gene="mhpF"
                     /locus_tag="ACHKV2_001945"
     CDS             20013..20963
                     /gene="mhpF"
                     /locus_tag="ACHKV2_001945"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO6939831.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            20960..21973
                     /gene="mhpE"
                     /locus_tag="ACHKV2_001946"
     CDS             20960..21973
                     /gene="mhpE"
                     /locus_tag="ACHKV2_001946"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO6939832.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            22149..23360
                     /gene="mhpT"
                     /locus_tag="ACHKV2_001947"
     CDS             22149..23360
                     /gene="mhpT"
                     /locus_tag="ACHKV2_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO6939833.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            23462..24001
                     /gene="yaiL"
                     /locus_tag="ACHKV2_001948"
     CDS             23462..24001
                     /gene="yaiL"
                     /locus_tag="ACHKV2_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO6939834.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(24126..24959)
                     /gene="fghA"
                     /locus_tag="ACHKV2_001949"
     CDS             complement(24126..24959)
                     /gene="fghA"
                     /locus_tag="ACHKV2_001949"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6939835.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(25052..26161)
                     /gene="frmA"
                     /locus_tag="ACHKV2_001950"
     CDS             complement(25052..26161)
                     /gene="frmA"
                     /locus_tag="ACHKV2_001950"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO6939836.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(26196..26471)
                     /gene="frmR"
                     /locus_tag="ACHKV2_001951"
     CDS             complement(26196..26471)
                     /gene="frmR"
                     /locus_tag="ACHKV2_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO6939837.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26631..27677)
                     /gene="fbpC"
                     /locus_tag="ACHKV2_001952"
     CDS             complement(26631..27677)
                     /gene="fbpC"
                     /locus_tag="ACHKV2_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO6939838.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(27689..29767)
                     /locus_tag="ACHKV2_001953"
     CDS             complement(27689..29767)
                     /locus_tag="ACHKV2_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6939839.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(29836..30867)
                     /locus_tag="ACHKV2_001954"
     CDS             complement(29836..30867)
                     /locus_tag="ACHKV2_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6939840.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(30864..32180)
                     /gene="uhpC"
                     /locus_tag="ACHKV2_001955"
     CDS             complement(30864..32180)
                     /gene="uhpC"
                     /locus_tag="ACHKV2_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6939841.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(32253..33794)
                     /locus_tag="ACHKV2_001956"
     CDS             complement(32253..33794)
                     /locus_tag="ACHKV2_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6939842.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(33794..34423)
                     /locus_tag="ACHKV2_001957"
     CDS             complement(33794..34423)
                     /locus_tag="ACHKV2_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO6939843.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            34726..35688
                     /gene="tauA"
                     /locus_tag="ACHKV2_001958"
     CDS             34726..35688
                     /gene="tauA"
                     /locus_tag="ACHKV2_001958"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6939844.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFSKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35701..36468
                     /gene="tauB"
                     /locus_tag="ACHKV2_001959"
     CDS             35701..36468
                     /gene="tauB"
                     /locus_tag="ACHKV2_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO6939845.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36465..37292
                     /gene="tauC"
                     /locus_tag="ACHKV2_001960"
     CDS             36465..37292
                     /gene="tauC"
                     /locus_tag="ACHKV2_001960"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO6939846.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            37289..38140
                     /gene="tauD"
                     /locus_tag="ACHKV2_001961"
     CDS             37289..38140
                     /gene="tauD"
                     /locus_tag="ACHKV2_001961"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO6939847.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(38247..39221)
                     /gene="hemB"
                     /locus_tag="ACHKV2_001962"
     CDS             complement(38247..39221)
                     /gene="hemB"
                     /locus_tag="ACHKV2_001962"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO6939848.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9064 a        10387 c        10811 g         9111 t
ORIGIN      
        1 cgacaccgtg ctcagtgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga
       61 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatacg
      121 acgcaggcgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      181 ccgcatccga caccgtgcgt agttgcctga tgcgacgctg acgcgtctta tcatgcctac
      241 ccactgtttt tacaccgata attttccccc cacctttttg cactcattca tataaaaaat
      301 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      361 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      421 ggccggtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      481 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      541 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      601 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      661 gtgttaaagg ctcatggctg ccttcactgc tgctgggcgg cacccaggtt ggctggtttg
      721 gcgtcggtgt ggcgatgttt gctattccgg tgggcaaggc aaccgggctg gatattaatt
      781 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttctttggc atttcggcgc
      841 tgacggttct ttcgttgatt gcggttccgg ctatcgcctg tcttggcggt tattccgtgt
      901 ggctggccgt taacggcatg ggcggcctgg acgccttaaa agcggtcgtt cccgcacaac
      961 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggcacgc
     1021 tcaccgctga ctttgtccgc tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1081 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1141 tggggatggc ggatatctct gatgtgatga ttgctcaggg cctgctgttg cctgcgattg
     1201 tggtgctggg gctaaatatc tggaccacca acgataacgc gctctatgcg tcgggtttag
     1261 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1321 gtacggtctg tgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1381 cagctattcc tccagtgggt ggcgtgatca ttgccgacta tctgatgaac cgtcgccgct
     1441 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1501 ccctggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1561 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1621 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1681 ttgccaggca aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1741 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     1801 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     1861 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     1921 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     1981 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2041 ctgaaagcaa tgctggaagt gaagctggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2101 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2161 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2221 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2281 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2341 gcgcaccgtg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactct
     2401 tataacgggg cgtatacctc acgtctgttc cgcttgctga aaatgtccgg tattaacttt
     2461 gtcgccaacc cgctggtcaa tattcatctg caaggacgat tcgatacgta tccaaaacgt
     2521 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2581 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2641 atggggctgc atgtttgcca gctgatgggc tatgggcaga ttaacgatgg cctgaattta
     2701 atcacccacc acagcgccag gacgttgaat ttgcaggatt acagcattgc cgccggaaac
     2761 agcgccaacc tgattatcct gccggctgaa aatggatttg atgcgctgcg ccgtcaggtt
     2821 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     2881 accgtatatc tggagcagcc ggaagccatc gattacaaac ggtgatcggc tgggctgtgt
     2941 gttgacaaag cgagcgtagt ttatgccgga tgcggcttga acgccttatc aggcctacgt
     3001 agagcactga actcgtaggc ctgataagcg taacgcatca ggcaattgtg tcagtggtta
     3061 ccgcgattca ttctcgccaa catccgcaca ttcctccaac gccatgtgca aaaatgcctt
     3121 cgcggcggct gtctgccagc tatttttccg ccgcaacaaa accgccgttc tctccagtag
     3181 tggcggggca agaggaatcg ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa
     3241 caatgtggaa agggaagtgc ggcgaatcag ctccagaacc gcgctaattg agttcgcctc
     3301 aatgaccacc tgtggatgca gccccgcttt ctcgcagtag tggtcaatct gctctctggt
     3361 ggcaaattcc gcgctgagca ggaccagttt ttcatcatgc aagcgactca acgccacctg
     3421 ttcacaggca gccagcggat gatgttgcgc cacgactaac gctaaacttt ctgtcagtaa
     3481 aggaattgcc tccagctccg gcaaatgcac aggcgcgaag gcaatcccaa cgtccagctc
     3541 gtcgcgacaa agcagatctt cgattttctc ctgcgacatt tcctgtagct ggagcgtgat
     3601 gccggggtag cgcgcataga aatccgccat taaggggccg ataaagtagc tcgtaaaggt
     3661 gggggtgacg gcgatacgca gcgatcctct cgtcagatcg gcaacatcat gaatcgcccg
     3721 tttacccgct cccagttcct gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc
     3781 atcagtgaga tgaatcgttc gcccgctacg gtcaaacagc ggcacgccta aactctcctc
     3841 taactggcga atctgctggg aaagcgcagg ctgagagacg tgcaacgcac tggcggcacg
     3901 ggtgaagctg ccatgttcag ccacggcaag aaaataattg atatgtcgag agagcattcg
     3961 ccaacctata agtaaattca atagaactca tcataaatga gacttttacc ttatgacaat
     4021 cggcgagtag tctgcctctc attccagaga cagatagagg ttaacggtga aagagattat
     4081 tgatggattc cttaaattcc agcgcgaggc atttccgaag cgggaagcct tatttaaaca
     4141 gttggcgaca cagcaaagcc cgcgcacact ttttatctcc tgctccgaca gccgtctggt
     4201 ccctgagctg gtgacgcaac gtgagcctgg cgatcttttc gttattcgca atgcgggcaa
     4261 tatcgtccct tcctacgggc cggaacccgg tggcgtttct gcttcggtgg agtatgccgt
     4321 cgctgcgctt cgggtatctg acattgtgat ttgcggtcat tccaactgtg gcgcgatgac
     4381 cgccattgcc agctgtcagt gcatggacca tatgcctgcc gtctcccact ggctgcgtta
     4441 tgccgattca gcccgcgtcg ttaatgaggc gcgcccgcat tccgatttac cgtcaaaagc
     4501 tgcggcgatg gtacgtgaaa acgtcattgc tcagttggct aatttgcaaa ctcatccatc
     4561 ggtgcgcctg gcgctcgaag aggggcggat cgccctgcac ggctgggtct acgacattga
     4621 aagcggcagc atcgcagcgt ttgacggcgc aacccgccag tttgtgccac tggcagctaa
     4681 tcctcgcgtt tgtgccatac cgctacgcca accgaccgca gcgtaacctt atttttaaac
     4741 catcaggaga tgcaccatga ttcagtcgca aattaaccgc aatattcgtc ttgatcttgc
     4801 cgatgccatt ttgctcagca aagctaaaaa agatctctca tttgccgaga ttgccgacgg
     4861 caccgggctg gcagaagcct ttgtgaccgc ggctttgctg ggtcagcagg cgcttcctgc
     4921 cgacgccgcc cgccaggtcg gggcgaagct ggatctcgac gaagacgcca ttctactgtt
     4981 acagatgatc ccactgcgtg gctgcattga tgaccgtatt ccaaccgacc caacgatgta
     5041 tcgtttctat gaaatgttgc aggtgtacgg cacaaccctg aaagcgttgg ttcatgagaa
     5101 atttggcgat ggcattatca gcgcgattaa cttcaaactc gacgttaaga aagtggcgga
     5161 cccggaaggt ggcgaacgtg cggtcatcac cttagatggt aaatatctgc cgaccaaacc
     5221 gttctgacag ccatgcgtaa ccatcaaaag acgttcacga tgctgctggt tctggtgctg
     5281 attggtctta atatgcgacc actgctcacc tccgtcgggc cactgctacc gcaattgcgc
     5341 caggcaagcg gaatgagctt tagcgtggct gccctgttga ccgctctgcc ggtggttacc
     5401 atgggcgggc tggcgctggc cggaagctgg cttcatcagc atgtcagcga acgtcgcagc
     5461 gtcgccatca gtttgttact gattgccgtc ggtgcattga tgcgtgagct ttaccctcaa
     5521 agtgcgctgc tgcttagcag cgcactgctc ggtggggtgg ggatcggcat cattcaggcg
     5581 gtgatgcctt cggtgattaa acggcggttt cagcagcgca cgccactggt gatggggctg
     5641 tggtccgcgg ctctgatggg cggcggtggg cttggtgccg ccctgacacc ctggttagtt
     5701 caacatagcg aaacctggta tcaaacactc gcctggtggg cgctgcctgc cgttgttgcg
     5761 ctctttgcct ggtggtggca aagcacccgc gaggtcgcct cttcccacaa gacaacaacc
     5821 attccggttc gcgtggtttt cactcctcgc gcgtggacgc tgggtgttta cttcggtctg
     5881 attaacggtg gttacgccag cctgattgcc tggttacccg ccttctatat tgagattggt
     5941 gccagcgcgc agtacagcgg ttccttgctg gcattgatga cgcttgggca agccgcagga
     6001 gctttgctga tgcctgctat ggctcgccat caggatcggc gcaaactgtt aatgctggcg
     6061 ctggcgttac aactggtggg tttctgcggc tttatctggc tgccgctgca attgccggta
     6121 ttgtgggcga tggtgtgtgg tttaggtctg ggcggcgcat ttccgctctg tttgctgctg
     6181 gcgctcgatc actctgtgca accggctatt gctggcaagc tggtggcgtt tatgcaggga
     6241 atcggtttta tcatcgccgg gcttgccccg tggttttctg gcgtgctgcg tagtatcagc
     6301 ggcaattact tgatggactg ggcgtttcat gcgctgtgcg tcgtagggct gatgattata
     6361 accctgcgtt ttgcaccagc acgttttccg cagctgtggg tcaaagaggc atgatgcgac
     6421 gcttgtttct gcgctttgtt cacgccggat gcggcctaca tagatcactg aatttgtagg
     6481 cctgataagc gaagcgtatc aggcaatttt tataatttaa actgacgatt caactttgta
     6541 atctctgaaa taatattgct tatcccggtc gtttatttcg cgaataaccc gacaaggaac
     6601 gccagccgcc acgacgtttg gtggaatgtc ttttgtgacg atactacccg cgccaataac
     6661 agaattatcc ccaatggtga cgcctggatt aataaccaca tgacttccga tccagacgtt
     6721 attgccaatc gttatcggaa aagagtacat ctcaccgttt tttctcaatt catggtgtac
     6781 agggtgtccc gtaacggaaa gagtaacgtt gggtgcaatc aatacgttat caccgattgt
     6841 taccgtgtag tcatcgacaa tggttaaatt gaaatttgca taaaaattgc ggcctatatg
     6901 gatgttggaa ccgtaagaga aatagacggg gggttctacc caggcgtttt cccctaccgt
     6961 ggcaaacatt tctttaatca ggctttctct tttatcaact tctgatggat gcgagtgatt
     7021 aaactcatac attaacgttt ttctacgaag tcttttttca ggtaagcctt cgcacatatc
     7081 ggtaaatagc ttgcctgctt ttattctttc tgtcatcgac atgttcattg cgatcattcc
     7141 gttatgatat gttggtcgga taaggcgctc gcgccgcatc cgacattgat tgcttaagcg
     7201 acttcattca cctgacgacg cagtagagaa agcgggccgg ggccgctaag cgtgaacacg
     7261 gaaattaagg tgaagcccag cgccaccaga cccagcacca gataagcgcc ctggaaaccg
     7321 atgctttcat acatattacc cgctagtaca gacataaaaa tcatcgccag ttgcttaaag
     7381 aagcagaaac agaccagata aatcgtcgct gaaaaacgca cttcaaactg gctggtaata
     7441 tatttaaagc agcccaccag caggaacggt acttcaaaca tatgcagcgt tttcagaata
     7501 accacttcca gcgctgaggt ggcgaacgat gagccaataa tacgtacaga cataatagtg
     7561 ccagccagca gcagggcatt tttcccaccg atgcgattaa tgatcagtgg cgcaaagaac
     7621 ataattgagg cgttaagtaa ttcgcccatt gtcgttacgt agccaaatac ccgcgtaccc
     7681 tgttcaccgg tggcaaagaa agaagtaaag aaattagcaa actgttggtc aaaaacatcg
     7741 taggtgcagg aaacgccaat aacatacagt gacaaaaacc acagttttgg ctgtctgaac
     7801 agttccagcg ccagtttaag gctaaatgcc gaatggttgg cacctaccgc attggcaacc
     7861 gtggcggaag agggcgcatc cgttttggcg aaaaagagta aaatggcgag gatgagtgca
     7921 cagccagaac ccagccagaa aacgaactga ttattgatgg tgaacatgat gccgacaatc
     7981 gaggcacaca gcgcccagcc aacacagcca aacatccgcg cgcgaccaaa ttcgaaatta
     8041 ctgcgacggc tgactttctc gataaatgcc tctactgcgg gcgcaccggc gttaaaacaa
     8101 aagccaagat aaataccacc aacaatcgat cctactaaaa tgttgtattg taacagtggc
     8161 ccgaagataa aaataaagaa cggcgcaaac atcactaaca tgccggtaat aatccacagc
     8221 aggtatttgc gcagcccgag tttgtcagaa agcagaccaa acagcggttg gaataatagc
     8281 gagaacagag aaatagcagc aaaaataata cccgtatcac ttttgctgat atggttgatg
     8341 tcatgtagcc aaatcgggaa aaacgggaag taggctccca tgataaaaaa gtaaaagaaa
     8401 aagaataaac cgaacatcca aaagtttgtg ttttttaaat agtacataat ggatttcctt
     8461 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
     8521 tggtaatggt agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag
     8581 tcgtcgccac caatccccat atggaaaccg tcgatattca gccatgttcc ttcttccgcg
     8641 tgcagcagat ggcgatggct ggtttccatc agttgttgtt ggctgtagcg gctgatgttg
     8701 aactggaagt cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc
     8761 agaccgtttt cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag
     8821 cggtcaaaac aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc
     8881 cagtttaccc gctctgctac ctgcgccagc tggcaggtca ggccaatccg tgccggatgc
     8941 ggtgtatcgc tcgctacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc
     9001 cggtaggttt tccggctaat aaataaggtt tttccctgat gctgccatgc gtggacagtg
     9061 gtaatcagca ccgcgtcggc aagtgtatct gccgtgcact gcaacaacgc cgcttctgcc
     9121 tggtaatggc ccgcagcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct
     9181 tcacttacgc caatgtcgtt atccagcggt gcgcgggtga actgatcgcg cagcggcgtc
     9241 agcagttgtt ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat
     9301 tgccaacgct tattatccag ctcgatgcaa aaatccgttt cgctggtggt cagttgcggg
     9361 atggcgtggg gcgcggaggg gagtgtcacg ctgaggtttt ccgccagacg ccactgctgc
     9421 caggcgctga tgtgtccggc ttctgaccat gcggtcgcgt tcggttgcac tacgtgtacc
     9481 gttagccaga gttgcccggt gctctccagt cgcggtagtt caggcaattc aattacctgt
     9541 ttaccttgcg gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc
     9601 gccaccgtcc agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact
     9661 tcgatggttc gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc
     9721 agcgccggat gcggcgtgcg gtcggcaaag accagaccgt tcatgcagaa ctggcgatca
     9781 ttcggcgtat cgccaaaatc accgccgtaa gccgaccacg gattgccatt ttcatcatat
     9841 ttaatcagcg actgatccac caagtcccag acgaagccgc cctgtaaacg ggggtactga
     9901 cgaaacgcct gccagtattt agcgaagccg ccaagactgt tacccatcgc gtgggcgtat
     9961 tcgcaaagga tcagtgggcg catttctcca ggcagcgaaa gccatttttt gatggaccat
    10021 ttcggcacag ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata
    10081 tcggttgcgg aggtgtcggc tccgccgcct tcatactgca ccgggcggga tggatcgaca
    10141 gatttaatcc agcgatagag tgcgtcgtga ttagcgccgt ggcctgactc attccccagc
    10201 gaccagatga tcacactcgg gtgattacga tcgcgctgca ccattcgtgt tactcgttcg
    10261 ctcatggccg gtaaccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg
    10321 gtttcaatat tggcttcatc caccacatac aggccgtagc ggtcgcacag ggtgtaccac
    10381 agcggatggt tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcattagc
    10441 aggatatcct gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg
    10501 tgacggttaa cgccgcgaat cagcaacggc ttgccgttca gcagcagcag accattttca
    10561 atccgcacct cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg
    10621 gtgtgcagtt caaccaccgc acgatagata ttcgggattt cggcgctcca cagtttcggg
    10681 ttttcgacgt tcagacctag tgtgacgcga tcggcataac cgccacgctc atcgataatt
    10741 tcaccgccga aaggcgcggt gccgctggcg acctgcgttt caccctgcca caaagaaacc
    10801 gtcacccgca gctcatcgcg cagctcgccg tacatctgaa cttctgcctc cagtaccgcg
    10861 cggctaaaat catcattaaa gagagtggca acatggaaat cgctgatttg tgtggtcggt
    10921 ttgtgcagca acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc
    10981 agataactgc cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt
    11041 aaaaatgcgc tcaggtcaaa ttcagacagc aaacgactgt cctggccgta accgacccag
    11101 cgcccgttgc accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg
    11161 ccttcctgta gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc
    11221 tccgtgggaa caaatggcgg attgaccgta atgggatatg tcacgttggt gtagatgggc
    11281 gcgtcgtaac cgtgcatctg ccagtttgag gggacgacga cagtatcggc gtcaggaaga
    11341 tcgcactcca gccagctttc cggaaccgct tctggtgccg gaaaccagac aaattgccac
    11401 tcaccattca ggctgcgcaa ctgctgggaa gggcgattgg tgcgggcctc ttcgctatta
    11461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11521 tcccagtcac gacgttgtaa tacgacggcc agtgaatctg taatcatagt catagctgta
    11581 tcctgtgtga aattgttatc cgctcacaat ttcacacaac atacgagccg gacgcataga
    11641 gtgtaaagcg tggggtgcct aatgagtgag ctgactcaca ttaattgcgt tgcgctcact
    11701 gcccgctttc cagtcgggaa acttgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11761 ggggagaagc ggtttgcgtc ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    11821 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    11881 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacacg
    11941 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcaacccgg
    12001 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    12061 tgggaacgat gccctcattt agcatttgca tggtttgttg aaaaccggac atggcactcc
    12121 agtcgccctc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    12181 ccgccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    12241 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
    12301 taatactgtt gatgggagtc tggtcagaga catcaagaaa taacgccgga acattagcgc
    12361 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    12421 tgacgcgttg cgcgagaaga ttgtgcactg ccgctttaca ggcttcgacg ccgcttcgtt
    12481 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    12541 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    12601 gtttccccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    12661 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctgattcacc acgcgggaaa
    12721 cggtctgata agagacaccg gcatactctg cgacatcgta tagcgttact ggtttcacat
    12781 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    12841 gccattcggc ggggctgacg ccgaagtgag atttaaaatg ctgacgccag gtcacaggtg
    12901 agagaaaacc cacctcagcc gctatccgct caacgggcaa ctgactggat cccaacagtt
    12961 cctggctacg gcgtaagcgt tcagtaatga gccattcggc gatactcgaa ccggtcgctt
    13021 tcataaaatg gcgagttaat gtgcggcgac tcatcattac tcgctgcgcc agcgagtcga
    13081 gatcatgctg ttgcgcaatg ttttgccgca ggtaatccag caggaggttt atgcgttcat
    13141 catgggtatt tttcggcacc ggttgctcaa taaactgcgc ctgaccgccg gtgcgatatg
    13201 gcggtacgac catccgtcgg gcaatatggt tagcatagtc gctgccaaaa tgttgccgaa
    13261 caatatacaa acagcaatcc agcgccgcgg cagtaccggc agaggtaata atgccgtcat
    13321 cgtctacgta caacgcgtta atatccagat gtgtttgtgg aaaacgtgcc tgaaattcac
    13381 gctcgaactc ccagtgcgtg gcggcacgct tatttttcag taaacctgca tagccgagca
    13441 caaacgtgcc caggcaaagc ccgacaatct gtgcgccgtt atcccgtgcg gtcgttaagg
    13501 cttccagcag tttttgtggc ggtttttgtg taatggttcc ccagtaagga attatcacaa
    13561 tatctgcctg gatgactgcg gtatagtcat gggtggcggt tacggaaaac ccatcggcag
    13621 agtccacgtt tcccggcttt tcagcgcaaa taatcacgtg aaagcgtttt ttctccgcca
    13681 tcttttcact aaacacgata aacggtacgg aaaggtgaaa aggactaaaa ccggcaacgg
    13741 cgataatggc aacagttagc gggcgcataa cactctttat ctccgaatta gaatgtcaac
    13801 gtttcaccat cttctggtac gttcacaaat tcgataattt gattatctct ggcgtattcc
    13861 ttgagcgcat cacgcgtcag caaacagtgg tttacggcct ccatatgtgt cgcgataatt
    13921 tgcgcttctg ggagggtaaa atgtgttttg agaatatctt ctgctcccat aattatcgcg
    13981 ccatgcccca gaacatgcgc aaatccggca ttcaacacaa ccacttctgg ttggaaagtt
    14041 agcattgtag cctcgacgtc gtcacgccag agggtatcgc cagccagata gagcgttttc
    14101 tctgtcgggt gctggaaaac aacgccgcaa gctgcgccta aacgcttcgc catcgccggg
    14161 tcggcatagg tgcgatcgga gccgtgctgc cctgtcgttt tatgcagagt aatatcgcca
    14221 aatttagtct cgtcggtaag tacggtaaga tgcgtaaatc cttgtttatt cagtgattca
    14281 gcatcttgct cattttgcac ataaatcagt ttatctttag ggacgacgcg ggaggctgtt
    14341 tcgtcccagt gatcttcatg caaatgggtc acgattaggg cgtcaacgtt gagtaacgtt
    14401 gttaaatcgc acggtaattc taccgttgga ttgcggattt ctgcgcgtgc cgttccggca
    14461 aaacccggat aacttccttt aggcgcgagt aacgggtcga taagaaatgt tttgccagcg
    14521 tattggataa tttgggtggc attacgaatt tgcgtaattt tcatgatttg ctccacatca
    14581 cagtattgat gtcattagcg tagcgtgcag cccgtactgt gcatgtgggc aaatgggcga
    14641 ataacgatga gaatgggcca aattacacat tggcgtaact agcgtaaatg cacgccgctt
    14701 cgccgtccgg ccaccagaat agcctgcgat tcaacccctt cttcgatctg ttttgctacc
    14761 cgttgtagtg ccggaagatg cttttccgct gcctgttcga tggtcattgc gctcgccata
    14821 tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac ggcgatagag
    14881 gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtaaccctc tttgcgcgcg
    14941 cgcgccagaa tggcttccag ctttaacggt tctcgtgcca gttgatagtc atcaccgggg
    15001 cgggaggcta acatctcgat taattccttg cgttcttgtt ccgggcaaaa ggccagccag
    15061 gtcaggcccg aggcggtttt cagaagcggt aaacgccgcc cgaccattgc ccggtgaaag
    15121 gataagcggc tgaaacggtg agtggtttcg cgtaccacca ttgcatcaac atccagcgtg
    15181 gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagtgg ggccgccagt
    15241 gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcaga
    15301 cgaaaactat catcggaggg actacgccgg acatatccct cttcctgcag cgtctccagc
    15361 agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc gacgctggca
    15421 ccgccgtcaa gtttatttaa catatttaat aacattagac cgcgggttaa gccgcgcacg
    15481 gttttgtatt ccgtctgctc attgttctgc atattaattg acatttctat agttaaaaca
    15541 acgttgtgca cctggtgcac attcgggcat gttttgattg tagccgaaaa caccctttct
    15601 atactgagcg cacaataaaa aatcatttac atatttttaa caaaataagt tgcgctgtac
    15661 tgtgcgcgca acggcatttt gtccgagtcg tgaggtactg aaatggcaat acaacaccct
    15721 gacatccagc ctgctgttaa ccatagcgtt caggtggcga tcgctggtgc cggtccggtc
    15781 gggctgatga tggcgaacta tctcggccag atgggcattg acgtgctggt ggtggagaaa
    15841 ctcgataagt tgatcgacta cccgcgtgcg attggtattg atgacgaggc gctgcgcacc
    15901 atgcagtcgg tcggcctggt cgataatgtt ctgccgcata ctacgccgtg gcacgcgatg
    15961 cgttttctca ccccgaaggg ccgctgtttt gctgatattc agccaatgac cgatgaattt
    16021 ggctggccgc gccgtaacgc ctttattcag ccgcaggtcg atgcggtgat gctggaaggg
    16081 gtgtcgcgtt ttccgaatgt gcgctgcttg ttttcccgcg agctggaggc cttcagtcag
    16141 caagatgacg aagtgacctt gcacctgaaa acggcagaag ggctgcggga aatagtcaaa
    16201 gcccagtggc tggtggcctg tgatggtggg gcaagttttg tccgtcgcac cctgaatgtg
    16261 ccgtttgaag gtaaaactgc gccaaatcag tggattgtgg tagatatcgc caacgatccg
    16321 ttaagtacgc cgcatatcta tttgtgttgc gatccggtgc gcccgtatgt ttctgccgcg
    16381 ctacctcatg cggtacgtcg ctttgaattt atggtgatgc cgggagaaac cgaagaacag
    16441 ctgcgtgagc cgcaaaatat gcgcaagctg ttaagcaaag tgctgcctaa tccggacaat
    16501 gttgaattga ttcgccagcg tgtctacacc cacaacgcgc gactggcgca acgtttccgt
    16561 attgatcgcg tactgctggc gggcgatgcc gcgcacatca tgccggtatg gcaggggcag
    16621 ggctataaca gtggtatgcg cgacgccttt aacctcgcat ggaaactggc gttggttatc
    16681 caggggaaag cccgcgatgc gctgctcgat acctatcaac aagaacgtcg cgatcacgcc
    16741 aaagcgatga ttgacctgtc cgtgacggcg ggcaacgtgc tggctccgcc gaaacgctgg
    16801 cagggtacgt tacgtgacgg cgtttcctgg ctgctgaatt atctgccgcc agtaaaacgc
    16861 tacttcctcg aaatgcgctt caagccgatg ccgcaatatt acggcggtgc gctggtgcgt
    16921 gagggcgaag cgaagcactc tccggtcggc aagatgttta ttcagccgaa agtcacgctg
    16981 gaaaacggcg acgtgacgct gctcgataac gcgatcggcg cgaacttcgc ggtaattggc
    17041 tggggatgca atccactgtg ggggatgagc gacgagcaaa tccagcagtg gcgcgcgttg
    17101 agcacacgct tcattcaggt ggtgccggaa gtgcaaattc ataccgcaca ggataaccac
    17161 gacggcgtac tacgcgtggg ggatacgcaa ggtcgcctgc gtagctggtt cgcgcaacac
    17221 aatgcttcgc tggtggtgat gcgcccggat cgctttgttg ccgccaccgc cattccgcaa
    17281 acactgggca agaccctgaa taaactggcg tcggtgatga cgctgacccg ccctgatgcc
    17341 gacgtttctg tcgaaaaggt agcctgatat gcacgcttat cttcattgtc tttcccactc
    17401 gccgctggtg gggtatgtcg acccggcaca agaggtactc gatgaggtca atggcgtgat
    17461 tgccagcgcc cgcgagcgta ttgcggcttt ctcccctgaa ctggtggtgc tgtttgcgcc
    17521 agatcactac aacggctttt tctatgacgt gatgccaccg ttctgtttag gcgttggagc
    17581 gacggcaatt ggtgatttcg gcagtgcggc aggagagctg cccgtgcctg tggagctggc
    17641 ggaggcctgt gcgcatgccg tcatgaagag cgggatcgat cttgccgttt cttactgtat
    17701 gcaggtggac cacgggttcg cccagccgct ggagttcctg ctcggtgggc tggataaggt
    17761 gccagttctg cctgtgttca tcaacggtgt cgccacgccg ctgcccggtt tccagcgtac
    17821 ccgcatgttg ggtgaagcca ttggacgttt caccagcact ctcaataaac gcgtgctgtt
    17881 cctaggttcc ggtgggcttt cccatcagcc gccggtgccc gaactggcga aagccgatgc
    17941 ccatatgcgc gaccgcctgt tggggagcgg gaaagattta cccgccagtg agcgcgaatt
    18001 gcgtcagcaa cgggtgatta gcgccgctga gaagtttgtt gaggatcaga gaactctgca
    18061 tccgctcaac ccgatttggg ataaccagtt catgactttg ctggagcagg gacgcataca
    18121 ggaactggat gccgtcagta acgaagagct ttccgccatt gccggaaagt cgacacatga
    18181 aatcaaaacc tgggtcgccg cttttgccgc tatttctgca tttggcaact ggcgtagcga
    18241 agggcgttat taccgcccaa tcccggagtg gattgccgga tttggctcgt taagcgccag
    18301 aacagagaac tgaatatgca ggagaagatg atgagttatc agccacaaac cgaagccgcc
    18361 accagccgtt ttctgaatgt agaagaagcg ggtaaaacgc tgcgcatcca ttttaatgac
    18421 tgcggacaag gcgacgaaac cgttgtcctg ctgcatggtt ccggcccggg tgctactggc
    18481 tgggcgaact tcagccgcaa tatcgatccg ctggtagagg cgggctatcg ggtgatcctg
    18541 ctggattgtc cgggttgggg caagagcgat tcgatcgtta atagtggttc gcgatcggat
    18601 cttaatgcac gaatcctgaa aagcgtggtg gatcaactgg atatcgccaa aatccacctg
    18661 ctgggcaact cgatgggtgg ccatagttct gtggcgttca cccttaactg gccggagcgc
    18721 gtcggcaaac tggtgctgat gggcggcggt acgggcggca tgagtttgtt tacgccgatg
    18781 ccaaccgaag gtattaagcg actgaatcag ctttatcgtc agccgactat cgaaaacctg
    18841 aagctgatga tggatatctt cgtttttgat accagcgatt tgaccgacgc cctgtttgaa
    18901 gcgcgcctga ataatatgct gtcgcgccgc gatcacctgg aaaacttcgt taagagcctg
    18961 gaagctaatc cgaaacagtt cccggatttt ggcccacgtc tggcggaaat caaagcgcaa
    19021 accctgattg tctgggggcg caacgaccgc tttgtgccga tggatgcggg tctgcgtctg
    19081 ctgtccggca ttgccggttc tgaactgcat atcttccgcg actgtgggca ttgggcgcag
    19141 tgggaacatg ccgacgcttt caatcaactg gtgctgaatt tcctcgcacg tgcttaagga
    19201 acggtcatga cgaagcatac tcttgagcaa ctggcggcgg atttacgccg cgccgcagag
    19261 cagggcgaag cgattgcgcc gctgcgcgat ctgattggta tcgataacgc tgaagcggcc
    19321 tacgccattc agcacataaa tgtgcaatat gacgttgcgc aggggcgtcg cgtggtgggg
    19381 cgtaaagtgg gcctgacaca tccgaaagtg cagcaacaac tgggcgttga tcaaccggat
    19441 ttcgggacat tatttgccga catgtgttat ggcgataacg aaatcattcc tttttcccgt
    19501 gttctgcaac cccgcattga agcggagatc gcactggtgt tgaaccgcga tttacccgca
    19561 actgatatca ccttcgacga attgtataac gccattgaat gggtacttcc ggcgctggag
    19621 gtggtgggta gccgcattcg cgactggtcg attcagtttg tcgataccgt ggcagataac
    19681 gcttcctgcg gggtgtatgt catcggcggt ccggcgcaac gtccggcggg gttagacctg
    19741 aaaaactgcg ccatgaagat gacgcgtaat aacgaagagg tttccagcgg gcgcggcagc
    19801 gaatgcctgg gacatccgct taatgccgcc gtctggctgg cacgcaaaat ggccagtctg
    19861 ggtgaaccgc tgcgcgccgg agatatcatt cttaccgggg cattaggtcc gatggtggca
    19921 gtgaatgcgg gcgatcgttt tgaagcccat attgaaggca taggttcagt tgctgcggca
    19981 ttttcaagcg cagccccaaa aggaagtctg tcatgagtaa gcgtaaagtc gccattatcg
    20041 gttctggcaa cattggcacc gatctgatga ttaaaatttt gcgtcacgat cagcatctgg
    20101 agatggcggt gatggtcggc attgatcctc agtccgacgg tctggcgcgt gccagacgta
    20161 tgggcgtcgc caccacccat gaaggggtga tcgggctgat gaacatgcct gaatttgctg
    20221 atatcgacat tgtatttgat gcgaccagcg ccggtgctca tgtgaaaaac gatgcggctt
    20281 tgcgcgaagc gaaaccggat attcgcttaa ttgacctgac gcctgctgcc atcggccctt
    20341 actgcgtgcc ggtggttaac ctcgaggaga acgtcgatca actgaacgtc aacatggtca
    20401 cctgcggcgg ccaggccacc attccaatgg tggcggcagt ttcacgcgtg gtgcgtgttc
    20461 attacgccga aattatcgct tctatcgcca gtaaatctgc cggacctggc acgcgtgcca
    20521 atatcgatga atttacggaa accacttcgc gagccattga agtggtgggc ggcgcggcaa
    20581 aagggaaggc gattatcgtg cttaacccag cagagccacc gttgatgatg cgtgacacgg
    20641 tgtatgtatt gagcgacgaa gcttcacaag atgatatcga agcctcaatc aatgaaatgg
    20701 ctgaggcggt gcaggcttac gtaccgggat atcgcctgaa acagcgcgtg cagtttgaag
    20761 ttatcccgca ggataaaccg gttaatttgc cgggcgtagg gcagttctcc gggctgaaaa
    20821 cggcggtctg gctggaagtc gaaggcgcag cgcattatct gcctgcctat gcgggcaacc
    20881 tcgacattat gacttccagt gcgctggcga cagcagaaaa aatggcccag tcactggcgc
    20941 gcaaggcagg agaagcggca tgaacggtaa aaaactttat atctcggacg tcacgttgcg
    21001 tgacggtatg cacgccattc gtcatcagta ttcgctggaa aacgttcgcc agattgccaa
    21061 agcactggac gatgcccgcg tggattcgat tgaagtggcc cacggcgacg gtttgcaggg
    21121 gtccagcttt aactatggtt tcggcgcaca tagcgacctt gaatggattg aagcagcggc
    21181 ggatgtggtg aagcacgcca aaatcgcgac gttgttgctg ccaggaatcg gcactattca
    21241 cgatctgaaa aatgcctggc aggctggcgc gcgggtggtt cgtgtggcta cgcactgtac
    21301 cgaagctgat gtttccgccc agcatattca gtatgcccgc gagctcggaa tggacaccgt
    21361 tggttttctg atgatgagcc atatgaccac gccggagaat ctcgcaaagc aggcaaagct
    21421 gatggaaggc tacggtgcga cctgtattta tgtggtggat tctggcggtg cgatgaacat
    21481 gagcgatatc cgtgaccgtt tccgcgccct gaaagcagtg ctgaaaccag aaacgcaaac
    21541 cggcatgcac gctcaccata acctgagtct tggcgtggcg aactctatcg cggcggtgga
    21601 agagggctgc gaccgaatcg acgccagcct cgcgggaatg ggagcgggcg caggtaacgc
    21661 gccgctggaa gtatttattg ccgccgtgga taaactgggt tggcagcatg gggccgatct
    21721 ctatgcgtta atggatgctg ctgacgacct ggtgcgtccg ttgcaggatc gaccggtacg
    21781 agtcgatcgc gaaacgctgg cgctgggata cgctggtgtt tactccagct tcctgcgtca
    21841 ctgtgaaacg gcggcggcgc gttatggctt aagcgcggtg gatattctcg ttgagctggg
    21901 caaacgccgg atggttggcg gccaggagga tatgatcgtc gacgtggcgc tggatctgcg
    21961 caacaacaaa taatattgac tgccgagaac gtgcattttg tatgcccatc ccagcacgac
    22021 tgccggatgc ggcgtgaacg ccttatccgg tctacatttc gcccgtaggc agtcattaaa
    22081 tagttctgat tacgcggctg gcaatgtatc agtcgcgatt cacctgcaca cgcaatgagg
    22141 ttcatcttat gtcgactcgt accccttcat catcttcatc ccgcctgatg ctgaccatcg
    22201 ggctttgttt tttggtcgct ctgatggaag ggctggatct tcaggcggct ggcattgcgg
    22261 cgggtggcat cgcccaggct ttcgcactcg ataaaatgca aatgggctgg atattcagcg
    22321 ccggaatact cggtttacta cccggcgcgt tggttggcgg aatgctggcg gaccgttatg
    22381 gtcgcaagcg cattttgatt ggctcagttg cgctgtttgg tttgttctcg ctggcaacgg
    22441 cgattgcctg ggatttcccc tcactggtct ttgcgcggct gatgaccggt gtcgggctgg
    22501 gggcggcgtt gccgaatctt atcgccctga cgtctgaagc cgcgggtcca cgttttcgtg
    22561 ggacggcagt gagcctgatg tattgcggtg ttcccattgg cgcggcgctg gcggcgacac
    22621 tgggtttcgc gggggcaaac ttagcatggc aaacggtgtt ttgggtaggt ggtgtggtgc
    22681 cgttgattct ggtgccgcta ttaatgcgct ggctgccgga gtcggcggtt ttcgctggcg
    22741 aaaaacaggc tgcgccacca ctgcgtgcgt tatttgcgcc agaaacggca accgcgacgc
    22801 tgctgctgtg gctgtgttat ttcttcactc tgctggtggt ttacatgttg atcaactggc
    22861 taccgctgct tttggtggag caaggattcc agccatcgca ggcggcaggg gtgatgtttg
    22921 cactgcaaat gggtgcggcg agcgggacgt taatgttggg cgcattgatg gataagctgc
    22981 gtccagtaac catgtcgcta ctgatttata gcggcatgtt agcttcgctg ctggcgcttg
    23041 gaacggtgtc gtcatttaac ggtatgttgc tggcgggatt tgtcgcgggg ttgtttgcga
    23101 caggtgggca aagcgttttg tatgccctgg caccgttgtt ttacagttcg cagatccgcg
    23161 caacaggtgt gggaacagcc gtggcggtag ggcgtctggg ggctatgagc ggtccgttac
    23221 tggccgggaa aatgctggca ttaggcactg gcacggtcgg cgtaatggcc gcttctgcac
    23281 cgggtattct tgttgctggg ttggcggtgt ttattttgat gagtcggaga ttaagaatgc
    23341 agccgtgtgc cgatgcctga cgcgccttat taagcagggg cgaaagggac ttgcccccaa
    23401 tacacccacg gcgtttacag gtatactcgc taaaaattat tcagcgggtt tggaaacaaa
    23461 gatggcaaaa cttaccttac aagagcagtt gctcaaagca ggattagtca ccagcaaaaa
    23521 agcggcgaag gtcgagagaa cggcgaaaaa atcgcgcgtt caggcgcgtg aagctcgggc
    23581 ggcggtagaa gaaaataaaa aggcacagct tgagcgtgat aaacagctta gcgaacagca
    23641 aaaacaagcg gcgttggcga aagaatataa agctcaggtg aagcagctta ttgaaatgaa
    23701 ccggatcacc attgccaatg gcgatattgg ttttaacttc actgatggca atctgattaa
    23761 gaagattttt gtcgataagc tcacacaggc gcagttgatt aatggtcgtc tggcgattgc
    23821 ccgcttgttg gtcgataaca atagcgaagg tgaatacgcc attattcccg ccagcgttgc
    23881 cgataaaatt gctcagcgtg atgccagcag tattgtgttg cacagcgcgc tcagtgccga
    23941 agagcaggat gaagacgacc cgtatgccga cttcaaagtg cctgatgatt tgatgtggta
    24001 aacatagata agtcgtgcgc agatgcctga tgcgacgcta tgcgcgtctt atcatgccta
    24061 cggcctgcgg gcgaaatgta ggcggataag gcgttcacgc cgcatccggc agtcgtgcac
    24121 tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc aataaaactg
    24181 gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt tttgatattc
    24241 atctcctggc agatcttttc aagattttga gtccgcagct gttccgcgta aaaatcatca
    24301 ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat aagactcacc
    24361 ggatggtaat ccaaccaggc ctctttattt tcaccaagat atgcggcaaa ggcttgctgt
    24421 ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct gacatattca
    24481 tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg accagagata
    24541 gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc gttgcggata
    24601 tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag gtaaaacccg
    24661 gcaccttgcc cgagatcgta acggtcggca tctgcgacat gactgcctcg cggactggtg
    24721 tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcacccc cgatttagta
    24781 atgaaattct gctcattgca ggtcaggccc gaaagccagt acagcaccgg taatttttca
    24841 cctgcagctt ttggtgggag atagacgccg acattcattt cacatttcag tgatgcggaa
    24901 taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat gagttccatc
    24961 agtcattatc tcacggttaa tcccccggac ggctgcccgg agagagtatt aagaaccccg
    25021 tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt cgaatggatt
    25081 tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg gtatgcgtga
    25141 caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc ggtaactggg
    25201 aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg accaactgga
    25261 atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc gactgacccc
    25321 agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg atgcattcaa
    25381 aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc ggtttgtcgt
    25441 agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg aatttcttcg
    25501 ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc tgaaccactg
    25561 ccagaccaat cgcgccaaga ccaaacacag caacagaatc ccctggctgg actttagctg
    25621 tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag acgtgttcat
    25681 ggtttgcttc tggattaatt tttgccagag acacttccgc gactacggtg tattcactga
    25741 atgtagaaca ccccatgtag tgataaagcg gctgcccatt gtaagaaaaa cgggtggtgc
    25801 cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg ttagttttgc
    25861 cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc acatggtcgc
    25921 ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc ccttcgtgac
    25981 cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat gcgtcggtat
    26041 ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt ggtgcaacgt
    26101 caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca cgtgatttca
    26161 tctctcgctc ttcctcaata tggtaataga ttcagctatt taagataggc acgaaccagt
    26221 tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt tcggtcaaac
    26281 gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc gccccgaacg
    26341 gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga ccgttccaga
    26401 gcatcaatct gccctcgaat acgacgaact cgagtaagga cctttttctt ctcttccgga
    26461 gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg gtatgctata
    26521 tgtcaatgca taccccccta tagtatataa caatggagaa taattgttgt ccgaagccta
    26581 accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt ttatgccgca
    26641 tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc accaatatcg
    26701 ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca ctctacggtc
    26761 acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg actctcttcg
    26821 ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct accttgcacc
    26881 gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc ttcgctaaag
    26941 ttcgccggga acaggttggc gtcgcccata aaacttgcca taaagcggga ggctggctga
    27001 cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat caccagcaca
    27061 gtgtcggaaa ccgcgaaggc ctcactttga tcgtgagtca cgtagagcga cgtgatatta
    27121 aactgctttt gcagctcgcg gatcttatcg cgcatgctgc ggcgcaggtt ggcatcgagg
    27181 ttacttaacg gctcatcaaa cagcagcacc tttggtttga ggatcaacgc gcgggccagc
    27241 gccacgcgct gttgctgacc accggaaatc tggtcgacat agcggtcctc gaacccttcc
    27301 agatccacca ttgccagcgc ctctttcacc cgctgcttta cttcgctacg cgacacgccg
    27361 agcatcttta acccgtagcc gacgttttcg cccagcgaca tatgcgggaa cagagcgtat
    27421 gactgaaaca ccatgcagat atcgcgctgc tgaatggaac gatgcgtgac atcttcgcca
    27481 tcaataaaga tttgcccttc actcggtttt tccagcccgg caaccagacg caaaatggtg
    27541 gttttgccgc agccggaagg gccaagcagc gtcaccattt gcccttgtgg gatggtgaga
    27601 ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg cagttccaca
    27661 aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta gaacgggaga
    27721 tacgtgcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc accacgatca
    27781 gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg ttaagaatgt
    27841 aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg accgtcgtga
    27901 tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc agcggcagta
    27961 aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct tcatccagcg
    28021 atttgtcgat ctgccccagg cctgcaatcc cggcgcgaat acccaccgga acgttacgca
    28081 tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga gcgctgttaa
    28141 aggcaagaat atacgaaacg cccgccaccg tacccggtac ggcaaagcac agcatggtgg
    28201 tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag gcgatgagca
    28261 ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta tccagcagtg
    28321 aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta tccagcgtca
    28381 gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg ccgtagagca
    28441 gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc agggtgaccg
    28501 gaagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa gagcgtttac
    28561 caatccacat atactgtatg cagaacacca gcagcgagaa cagcagcagg aaagcgccaa
    28621 gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa atttgcgtcg
    28681 ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc agtgactgta
    28741 ccacgacgat taaaaaggca ttggcgagcg cgggcttcaa caacggcaca aacacgccgt
    28801 taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc gagggatgaa
    28861 tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc agaacctgtg
    28921 ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca aaccactcca
    28981 ccataaattc cgtaacatag ccggaacgcc ccatcatgag cgtcacgcct aagccgacca
    29041 caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg accaataatg gcgctgcgtt
    29101 tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagcacag cctatcccca
    29161 ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc tgcgagagca
    29221 cggtcataaa cgccagcggg gcgagctctc ccgcgtcgtt ggtgaacatc gggataaaaa
    29281 tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca atcagtgagc
    29341 cgatcacgaa gcggtcgccg cccagccatt cgaggcgggt taaggccagc gtcataatcg
    29401 cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca gccacggtgg
    29461 cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa taatgacgac
    29521 tacgctgttc gcgcgtggcc tgacgaaacg ggcggaccag caacaggctc gggagtaaat
    29581 accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc tcatcactcg
    29641 tggattccag caggccataa tcgaggctcc agctcggcaa caggacaaat gccagccagc
    29701 ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt gcaagtgtct
    29761 gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca cccggcaaat
    29821 gcgcctaatg cgggtttatt tacccatctt cacttcgctg acccatttat taatcaatgc
    29881 cttacgaaca tccgtggaac catatttatc catgtcgtag ttgattaatt tcaggtcgtc
    29941 gagcttcagc gaatttggcg acgtttcggc ggtggtatta gtcaggatct gataagactt
    30001 ccctttcttc cacgccagtt cctgagcttc ttttgataat acccagtcca caaacagttt
    30061 ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt cgtagcccgt
    30121 accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca gcgagtagtc
    30181 atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg ccggggctat
    30241 gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat caaaggcctg
    30301 atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac cggaactttg
    30361 cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt tccagcactt
    30421 cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac cgaggatccc
    30481 cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga atttctccat
    30541 gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc cagcctgcga
    30601 ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct tttcagcatc
    30661 gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta catcatattt
    30721 ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc aatagaccac
    30781 cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg cagaagcaat
    30841 cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt ggtggtgatg
    30901 cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac cgagagtaaa
    30961 ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca gcctgccagc
    31021 gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc cgcagctttg
    31081 tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac gaaaaagccc
    31141 acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag ccacagtgcg
    31201 gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg ttgcccgcta
    31261 aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc cacttcaaac
    31321 agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt cagccagata
    31381 ttgccccagt cgttaagcgc aatccggatc acataaacca gtacatatga gacgcccagt
    31441 agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag ccccatcggc
    31501 ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc aactgtcggt
    31561 aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc taatgccatg
    31621 ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc ggcaaaggcg
    31681 ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca gccccaccag
    31741 aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg tggccatccc
    31801 cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag tgattcgcca
    31861 aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat aaaaccgcgt
    31921 tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta gctgagataa
    31981 aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt ttgtagcgcc
    32041 ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc aataatcatg
    32101 cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct cgctgaacgc
    32161 gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa ggggatgaga
    32221 gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg caaaaatgtg
    32281 ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc gccgcccagg
    32341 gcgctgacgc gctcacgcat cccctgaatg ccaaatccag ccattttccc gctggcgatc
    32401 cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg caggataatc
    32461 gtcacttcgc tggcttcggc gtgtttacag atgttattga gcaactcctg caataaccga
    32521 tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata ggcaaactgg
    32581 caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcagcaa cgcttcgcga
    32641 aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg ggtggagaga
    32701 tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg cgccggatca
    32761 cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg accgatttcg
    32821 tcgtgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat cagcttttcc
    32881 gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg gtgcaggttt
    32941 agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag caaggcctgg
    33001 gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat ctggcgggcg
    33061 atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca gccaaatttc
    33121 cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc aatttcaggt
    33181 gacagcacca tctgagtgcc gataccaata ataaaaaaca ggctacacca gatgattaaa
    33241 gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg atgctgctgg
    33301 cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc ggtaaatgag
    33361 gtcaaacccg gtagcattat tgggcctgac ataaaagggg agagcagggc ggtattcaac
    33421 aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta aagtggaaaa
    33481 cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg cgttgccagc
    33541 atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag caaagggggg
    33601 agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag ataaactccc
    33661 tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct gagcgtccac
    33721 agcatcagcc agccggttcc ccaggctagc acaataaaca gcaaaatcac ccaatgacga
    33781 agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa tgtaccagtt
    33841 caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca tgtaccgttt
    33901 tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg accagtaact
    33961 caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt tgcccgccgc
    34021 cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg accgagcgca
    34081 ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg gcaccggcat
    34141 ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg gcgcgaaatt
    34201 gcggtttttg tgcacgcagg cgcttaagca aactgaggcc gttttcgtcc ggcatggcaa
    34261 tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc tgcgcggcac
    34321 tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt gcgaagccag
    34381 agcgcaccac aacatggtca tccaccagca ctacccgaat catacttttc ttctcttatt
    34441 atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc gacagatatg
    34501 gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat ctggcttgtg
    34561 aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac cattgccgat
    34621 ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc aaactgaccc
    34681 gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc aatttcatcg
    34741 cgtaacactc ttcttgccgc actggcattc atcgcttttc aggcgcaggc ggtgaacgtc
    34801 accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga taacaccttt
    34861 gctaaagaaa gtggagcaac tgtggactgg cgcaagtttg acagcggagc cagcatcgtg
    34921 cgggcgctgg cttctggcga cgtgcaaatc ggcaaccttg gttccagccc gttagcggtt
    34981 gcagccagcc aacaggtgcc gattgaagtc ttcttgctgg cgtcaaaact gggtaactcc
    35041 gaagcgctgg tggtaaagaa aactatcagc aaaccggaag atctgattgg caaacgcatc
    35101 gccgtaccgt ttatctccac cacccactac agcctgctgg cggcgctgaa acactggggc
    35161 attaaacccg ggcaagtgga gattgtgaac ctgcagccgc ccgcgattat cgctgcctgg
    35221 cagcggggag atattgatgg tgcttatgtc tgggcaccgg cggttaacgc cctggaaaaa
    35281 gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc aacgctggac
    35341 gtttgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa agcgttctct
    35401 aaaagcgcca tcgatgctca gcaaccgtac attgctaacc cagacgtgtg gctgaaacag
    35461 ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga cgttccgggg
    35521 ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact gaccggaccg
    35581 gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa ggtcccggct
    35641 gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa ggaggcgcag
    35701 atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggtact ggaagatatc
    35761 aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg ctgcggtaaa
    35821 accaccctgc tgaatttgat tgccggtttt gtgccttatc agcatggcag cattcaactg
    35881 gcgggtaagc gtattgaggg gccgggagca gagcgtggcg tagtttttca gaatgaaggg
    35941 ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt ggcaggtata
    36001 gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg gctggaaggc
    36061 gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt ggggattgcc
    36121 cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg tgcgctggac
    36181 gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga gacgggcaag
    36241 caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac tgaactggtt
    36301 ctgctttcac ccggccctgg ccgcgtgctg gagcggctgc cgctcaactt tgctcgccgc
    36361 tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat cgccatgcgc
    36421 gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt gtgctcatta
    36481 atgaaaaact gcattcgcgg cggctgaaat ggcgctggcc gctctcgcgt caggtgacct
    36541 taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg gcgctgcaac
    36601 tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg ctcaccattg
    36661 ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc agcctgacgc
    36721 gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg atcgccatgg
    36781 ggctaagccc gacggtgcgc ggcattctgg atccgataat cgagctttat cgtccggtgc
    36841 cgccgctggc ttatttgccg ctgatggtga tctggtttgg tattggtgaa acctcgaaga
    36901 tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgctg gcgggggtga
    36961 aaagcgcgca gcaggttcgc attcgtgctg cccagtcgct gggtgccagc cgtgcgcagg
    37021 tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga ttacgtattg
    37081 gtctgggtgt gggctggtct acgctggtgg cggcggagct gattgccgcg acgcgcggtt
    37141 taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg ctggcgggga
    37201 tcgcggtgat tgcgattatc gcctttcttt tagaactggg tctgcgcgcg ttacagcgcc
    37261 gcctgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta ccccgctggg
    37321 gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgcccgttaa gcgataatca
    37381 gtttgaacaa ctttaccatg cggtactgcg ccatcaggtg gtgtttctgc gcgatcaagc
    37441 cattacgccg cagcagcaac gcgcgctggc ccagcgtttt ggcgaactgc atattcaccc
    37501 tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc ataacgataa
    37561 tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc cacccgcagg
    37621 ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct ggaccagcgg
    37681 tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg ggctgcgtgc
    37741 ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg aagaggaaca
    37801 tcaacgctgg cgcgaggcgg ttgcgaaaaa tccgccgttg ctacatccgg tggtgcgaac
    37861 gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta cgcgaattgt
    37921 tgatgtcagc gagaaagaga gcgaagcctt gttaggcttt ttattcgccc atatcactaa
    37981 accggagttt caggtgcgct ggcgctggca accgaatgat attgcgattt gggataaccg
    38041 tgtgactcag cactatgcca atgccgatta cctgccacag cggcggatta tgcatcgggc
    38101 gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg gacggtcctc
    38161 tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtgctttc aacaggttaa
    38221 ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc caaatccagc
    38281 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa gctttcgagc
    38341 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcatactca
    38401 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
    38461 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
    38521 agtgactcac gaatcgcctc acgacggttc attgggttca tctgatagct tttgcggtcg
    38581 ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga ggcgaacttg
    38641 gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc ctggcgaatc
    38701 gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc tgcagcagct
    38761 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
    38821 cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc tgacatgaca
    38881 atcatttctg gcaccgtctg cttgcagatg cgcgacatac gcgctaccag tccatcttcc
    38941 cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccgaaagt catcacggaa
    39001 cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
    39061 atcacgccag gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
    39121 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
    39181 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
    39241 gaattaaggt gcacagtata cctgaagcgg ggctggagtg ttttacgaaa gttgtcacga
    39301 tgttgacaag aagagaatga aagagagacc agggcatctt tgtaatagaa atagcaataa
    39361 cttcatattc act
//
LOCUS       DAZFTN010000035        38918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFTN010000035 DAZFTN010000000
VERSION     DAZFTN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            371..1144
                     /locus_tag="ACHKV2_001963"
     CDS             371..1144
                     /locus_tag="ACHKV2_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939849.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(1205..1759)
                     /gene="pinE"
                     /locus_tag="ACHKV2_001964"
     CDS             complement(1205..1759)
                     /gene="pinE"
                     /locus_tag="ACHKV2_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFO6939850.1"
                     /translation="MLIGYVRVSTNNQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGISTLYKKFPVGDK"
     gene            1790..2200
                     /locus_tag="ACHKV2_001965"
     CDS             1790..2200
                     /locus_tag="ACHKV2_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6939851.1"
                     /translation="MPVSHNLCIFLNVGLGEAAKRDVGTGENQIPDMASFASGDGWMK
                     LPNGKILQYGRGAVTPTLSTQTMRITFSIPFSKKADCAMLTHSGDGGAPLGAGRGFVM
                     TAEGPTLTGFNSAYRTSSTSDTVSMNYSWWAVGE"
     gene            2221..2664
                     /locus_tag="ACHKV2_001966"
     CDS             2221..2664
                     /locus_tag="ACHKV2_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6939852.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGEELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(2636..3238)
                     /gene="tfaE"
                     /locus_tag="ACHKV2_001967"
     CDS             complement(2636..3238)
                     /gene="tfaE"
                     /locus_tag="ACHKV2_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6939853.1"
                     /translation="MDNAVLNSELIATKAGNITVYNYDGKTREYISTSNEYLAIGVGI
                     PAYSCLDEPGIHKAGYAICRSMDLNSWEYVPDHRGEIVYNTETGDAKEITAPGDYPEN
                     TTTIAPLTPFDKWDGEKWVTDTEAQHGAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKPTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(3238..4008)
                     /locus_tag="ACHKV2_001968"
     CDS             complement(3238..4008)
                     /locus_tag="ACHKV2_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6939854.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVQTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGIDAGRVLLSIQTGMLEKHRHIVVANDGYDTKDEWELATIFKKTYTQGRGLDATNT
                     GGSLIPSPTLHSRGSIGNTGGSETRPRNIAFNYIVRAA"
     gene            complement(4012..4596)
                     /gene="ymfQ"
                     /locus_tag="ACHKV2_001969"
     CDS             complement(4012..4596)
                     /gene="ymfQ"
                     /locus_tag="ACHKV2_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFO6939855.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPATTNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSQTYVIFKYPE"
     gene            complement(4587..5645)
                     /locus_tag="ACHKV2_001970"
     CDS             complement(4587..5645)
                     /locus_tag="ACHKV2_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6939856.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIEPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPVD
                     NISIAKNELAVLGTISWT"
     gene            complement(5632..6057)
                     /locus_tag="ACHKV2_001971"
     CDS             complement(5632..6057)
                     /locus_tag="ACHKV2_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO6939857.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(6057..6605)
                     /locus_tag="ACHKV2_001972"
     CDS             complement(6057..6605)
                     /locus_tag="ACHKV2_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6939858.1"
                     /translation="MRTIEAMQRQLLGLIARAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNSPKARFEMDLEVTGQVKDLCDSSGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(6605..7684)
                     /locus_tag="ACHKV2_001973"
     CDS             complement(6605..7684)
                     /locus_tag="ACHKV2_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO6939859.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEVTTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7681..9009)
                     /locus_tag="ACHKV2_001974"
     CDS             complement(7681..9009)
                     /locus_tag="ACHKV2_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO6939860.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(9070..10905)
                     /locus_tag="ACHKV2_001975"
     CDS             complement(9070..10905)
                     /locus_tag="ACHKV2_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6939861.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(11047..11316)
                     /locus_tag="ACHKV2_001976"
     CDS             complement(11047..11316)
                     /locus_tag="ACHKV2_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6939862.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(11316..11672)
                     /locus_tag="ACHKV2_001977"
     CDS             complement(11316..11672)
                     /locus_tag="ACHKV2_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO6939863.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11672..13168)
                     /locus_tag="ACHKV2_001978"
     CDS             complement(11672..13168)
                     /locus_tag="ACHKV2_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6939864.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMSSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTMIASSIQDAINAVPTLPFTASSLA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVHTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(13152..13322)
                     /locus_tag="ACHKV2_001979"
     CDS             complement(13152..13322)
                     /locus_tag="ACHKV2_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO6939865.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(13331..13891)
                     /locus_tag="ACHKV2_001980"
     CDS             complement(13331..13891)
                     /locus_tag="ACHKV2_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939866.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDELGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            complement(13888..14394)
                     /locus_tag="ACHKV2_001981"
     CDS             complement(13888..14394)
                     /locus_tag="ACHKV2_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939867.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRRR"
     gene            complement(14369..14779)
                     /locus_tag="ACHKV2_001982"
     CDS             complement(14369..14779)
                     /locus_tag="ACHKV2_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6939868.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFRAWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGEFTQSHGGSNGDSLFSR"
     gene            complement(14776..15099)
                     /locus_tag="ACHKV2_001983"
     CDS             complement(14776..15099)
                     /locus_tag="ACHKV2_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6939869.1"
                     /translation="MLLKMEEIKLQLRLDDDFSDEDELLELLGKAAQSRTENFLNRKL
                     YATADDRPADDPDGLVISDDVKLALLLLVSHFYENRSTVTDVEKMELPMSFNWLVAPY
                     RLIPL"
     gene            complement(15178..16407)
                     /locus_tag="ACHKV2_001984"
     CDS             complement(15178..16407)
                     /locus_tag="ACHKV2_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6939870.1"
                     /translation="MKLHELKQKRNTIATDMRALNEKIGDNAWTEEQRTEWNKAKSEL
                     EALDERIAREEELRRQDQAYIESNEEEQRQNLDPENNPQQDEKRAQVFDKWMRHGASE
                     LTSEERKALRELRAQGVAQDEKGGYTVPETFLAKVVEKMKSYGGIASVAQILTTSDGR
                     TMEWATADGTSEVGVLLGENEEAGEEDTDFGMGSLGALKMTSKIIRVSNELLQDSAID
                     MEAYLARRIAERIGRGEARYLIQGTGAGTPKQPKGLVASVTGTTQTAAANTVKWQEIL
                     ALKHSIDPAYRRGPKFRLAFNDNTLKLISEMEDGQGRPLWLPDIVGVAPASVLNVPYV
                     IDQEIDDIGAGKKFMFCGDFDRFIIRRVRYMILKRLVERYAEYDQTGFLAFHRFDCIL
                     EDTSAIKALVGKGSVGG"
     gene            complement(16418..17020)
                     /locus_tag="ACHKV2_001985"
     CDS             complement(16418..17020)
                     /locus_tag="ACHKV2_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6939871.1"
                     /translation="MNDREIRCYSGEVRAERHDDNPAHIIGYGSVFDCRSELIFGSFR
                     EIIRPGAFDDVLGDDVRALFNHDPNFILGRSAAGTLNLSVDERGLRYDIQAPETQTIR
                     DLVLAPMQRGDINQSSFAFRVARDGEEWYQDEDGVVIREITRFSRLLDVSPVTYPAYQ
                     EADSAVRSMKAWQEARNSGALQKAINQRMARERVLTLLNA"
     gene            complement(17013..18239)
                     /locus_tag="ACHKV2_001986"
     CDS             complement(17013..18239)
                     /locus_tag="ACHKV2_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6939872.1"
                     /translation="MLLDALFRSKSLENPSTPITGDAVDTDGLFRTDVYVSPETAMKL
                     AAVYSCIYVLSSSLAQMPLHVMRRHNGKVEPARDHPAFYLVHDEPNTWQTSYKWRELK
                     QRHILGWGNGYTWVKRNRRGEVISLDCCMPWETTLMNTGGRYTYGLYNEYGAFAISPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQKYTESFFSGNARPAGIVSVKSGLNK
                     DSWGWLKDQWQKASQALRSQENKTMLLPADLDYKALTVSPVDAQIIDMMKLNRSMIAG
                     IFNIPAHMINDLEKATFSNISAQAIQFVRYTMMPWVTNWEQELNRRLFTRAELAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVEGLDEMLVSVNAANP
                     AGDFKPPKNDEGKTNE"
     gene            complement(18229..18390)
                     /locus_tag="ACHKV2_001987"
     CDS             complement(18229..18390)
                     /locus_tag="ACHKV2_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939873.1"
                     /translation="MKSLIADVIGLAGFGLLTCGFYLQFGMAPALMLSGGLLLVGALA
                     MARRGTRAA"
     gene            complement(18387..20120)
                     /locus_tag="ACHKV2_001988"
     CDS             complement(18387..20120)
                     /locus_tag="ACHKV2_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6939874.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWIDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTAGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPITIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAIGRAMLKEPDDFLS
                     SLDPDDALLIL"
     gene            complement(20117..20611)
                     /locus_tag="ACHKV2_001989"
     CDS             complement(20117..20611)
                     /locus_tag="ACHKV2_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO6939875.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(20737..21087)
                     /locus_tag="ACHKV2_001990"
     CDS             complement(20737..21087)
                     /locus_tag="ACHKV2_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6939876.1"
                     /translation="MPPRTPKACRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRGRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            21613..21906
                     /gene="iss"
                     /locus_tag="ACHKV2_001991"
     CDS             21613..21906
                     /gene="iss"
                     /locus_tag="ACHKV2_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO6939877.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(21997..22179)
                     /gene="rzoD"
                     /locus_tag="ACHKV2_001992"
     CDS             complement(21997..22179)
                     /gene="rzoD"
                     /locus_tag="ACHKV2_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO6939878.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(22393..22869)
                     /locus_tag="ACHKV2_001993"
     CDS             complement(22393..22869)
                     /locus_tag="ACHKV2_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO6939879.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(22873..23208)
                     /locus_tag="ACHKV2_001994"
     CDS             complement(22873..23208)
                     /locus_tag="ACHKV2_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO6939880.1"
                     /translation="MPHNPNTWPDWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGG
                     GGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVI
                     GNKFGGSKE"
     gene            complement(23345..23647)
                     /locus_tag="ACHKV2_001995"
                     /pseudo
     CDS             complement(23345..23647)
                     /locus_tag="ACHKV2_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(23917..24102)
                     /locus_tag="ACHKV2_001996"
     CDS             complement(23917..24102)
                     /locus_tag="ACHKV2_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939881.1"
                     /translation="MKHHEQIEIEAAKVVAELFAGNASPMETFGITWSQTQMLERKNP
                     GVVIKLVPDDGKKLAYC"
     gene            complement(24294..24836)
                     /locus_tag="ACHKV2_001997"
     CDS             complement(24294..24836)
                     /locus_tag="ACHKV2_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939882.1"
                     /translation="MMFQAFQTAVFKNVAEYLKGRFPHKSLGLVYADELVLKDMEFLK
                     ANNKLRWDPGLKSRVFMDMMEEHPIKLVVYYRGEPIGFAFGCYCKPKNAVHICWMEKR
                     NDAHEDLDHQMLGIVLDCFSAYARFLNLQGESIDSIALVSPVDGAIRYYTESGFEYIA
                     DYERGGCAMVLRSVLPNQSV"
     gene            complement(25048..25800)
                     /gene="ydfT"
                     /locus_tag="ACHKV2_001998"
     CDS             complement(25048..25800)
                     /gene="ydfT"
                     /locus_tag="ACHKV2_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6939883.1"
                     /translation="MNFEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHDVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
     gene            complement(25814..26803)
                     /locus_tag="ACHKV2_001999"
     CDS             complement(25814..26803)
                     /locus_tag="ACHKV2_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6939884.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(26811..27620)
                     /locus_tag="ACHKV2_002000"
     CDS             complement(26811..27620)
                     /locus_tag="ACHKV2_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO6939885.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(27640..28029)
                     /locus_tag="ACHKV2_002001"
     CDS             complement(27640..28029)
                     /locus_tag="ACHKV2_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6939886.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(28026..28352)
                     /locus_tag="ACHKV2_002002"
     CDS             complement(28026..28352)
                     /locus_tag="ACHKV2_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO6939887.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(28349..29002)
                     /locus_tag="ACHKV2_002003"
     CDS             complement(28349..29002)
                     /locus_tag="ACHKV2_002003"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO6939888.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRYELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(29002..29496)
                     /gene="yfdN"
                     /locus_tag="ACHKV2_002004"
     CDS             complement(29002..29496)
                     /gene="yfdN"
                     /locus_tag="ACHKV2_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6939889.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAVSGEEWYLSGN
                     YVGA"
     gene            complement(29493..30434)
                     /locus_tag="ACHKV2_002005"
     CDS             complement(29493..30434)
                     /locus_tag="ACHKV2_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6939890.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(30424..30603)
                     /locus_tag="ACHKV2_002006"
     CDS             complement(30424..30603)
                     /locus_tag="ACHKV2_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6939891.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(30779..31330)
                     /gene="ymfL"
                     /locus_tag="ACHKV2_002007"
     CDS             complement(30779..31330)
                     /gene="ymfL"
                     /locus_tag="ACHKV2_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO6939892.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(31323..31583)
                     /locus_tag="ACHKV2_002008"
     CDS             complement(31323..31583)
                     /locus_tag="ACHKV2_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6939893.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            31681..32373
                     /locus_tag="ACHKV2_002009"
     CDS             31681..32373
                     /locus_tag="ACHKV2_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6939894.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            32672..32947
                     /locus_tag="ACHKV2_002010"
     CDS             32672..32947
                     /locus_tag="ACHKV2_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939895.1"
                     /translation="MKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIMAGVVFSL
                     RRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            33223..33542
                     /locus_tag="ACHKV2_002011"
                     /pseudo
     CDS             33223..33542
                     /locus_tag="ACHKV2_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33624..34160
                     /gene="yfdR"
                     /locus_tag="ACHKV2_002012"
     CDS             33624..34160
                     /gene="yfdR"
                     /locus_tag="ACHKV2_002012"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6939896.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPQAPGHA
                     YGMFMERFNELSELRKCA"
     gene            34151..34513
                     /locus_tag="ACHKV2_002013"
     CDS             34151..34513
                     /locus_tag="ACHKV2_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939897.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            34513..34818
                     /gene="yfdT"
                     /locus_tag="ACHKV2_002014"
     CDS             34513..34818
                     /gene="yfdT"
                     /locus_tag="ACHKV2_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFO6939898.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            34734..35168
                     /locus_tag="ACHKV2_002015"
     CDS             34734..35168
                     /locus_tag="ACHKV2_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6939899.1"
                     /translation="MGYDSRLDRLAATSRYPFFNNVTTRGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            35090..36208
                     /locus_tag="ACHKV2_002016"
     CDS             35090..36208
                     /locus_tag="ACHKV2_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6939900.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(36223..36298)
                     /locus_tag="ACHKV2_002017"
     tRNA            complement(36223..36298)
                     /locus_tag="ACHKV2_002017"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36263..36265),aa:Thr,seq:cgt)
     gene            complement(36413..37666)
                     /gene="proA"
                     /locus_tag="ACHKV2_002018"
     CDS             complement(36413..37666)
                     /gene="proA"
                     /locus_tag="ACHKV2_002018"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6939901.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(37678..38781)
                     /gene="proB"
                     /locus_tag="ACHKV2_002019"
     CDS             complement(37678..38781)
                     /gene="proB"
                     /locus_tag="ACHKV2_002019"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6939902.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         9435 a        10696 c         9308 g         9479 t
ORIGIN      
        1 cgaatcaagt gctgaatgtc acagtatcga acagaaaaca gtgacgatct aacccttcaa
       61 gaatattcta cgattgttct gtttaggaaa agcaaggcgg gaagtcggga gataagtcat
      121 tgataaagtg gcggagagag ggggatttgc ccccccggta gagttgcccc tactccggtt
      181 ttcgagaccg gtccaatcat caaacgaaac ataaaattaa ttcacattat gaggaaaagt
      241 atcttttttg tactatgtaa attcaaagac ttagcctcat ttccccgatg attttctcaa
      301 cactactggt tgtgagccct tgcaatgttc attaatatac gtctcacgaa taattcatag
      361 atattgcaaa atggatatta ctgagtttcc ttctggagta attgaacacc ttggctggta
      421 tgtataccga ttgattgatc cgagggacgg aagcaccttc tatgtaggga aaggcaaagg
      481 taaccgcgta tttgcccata tgcgcggtga agtggcagcg actgatgatg acgagttact
      541 gagcaacaag ctaaagcaaa ttagagaaat aaggttagca ggacttgagg ttatccatgt
      601 catccatcga cacggaatga ctgatgaaaa gacggcgtac gaagtcgaag cagcacttat
      661 tgatgcctac cctgggttaa cgaatatcat gaatggtgct ggcagcaatg aattcggcgc
      721 cgcgcatgtc aaagagttga tagcaacata tcaacccgaa accataacat ttcatcataa
      781 agcattaatg atttccgtta acagaagtgc aaaggattca gagctttatg atgcggttcg
      841 atttagctgg cgcattaatg tctctcgcgc cagccaagca gaagtcattc ttgctactgt
      901 aagggggatc gttcgagggg ttttcattgc tgataaatgg ctcaaatcaa cacgtgaaaa
      961 tttccctacg atgaaatact gggacgagga tccggacttt gaggcaacac aaagttctcg
     1021 ctatggtttt gaaggtcgag aagccccacc tgaaatagca aatctttatc ttggaaaaaa
     1081 aataccagat gaattaagaa aaaaaggagc tatgtccccg gtccgttact cacctaattt
     1141 ttgagtcttt aagtgataag cataaaccgc agcacgatct tcttgcatac gacgtgctac
     1201 ggtttcattt atctccgacc ggaaacttct tatacagtgt cgatatacca acatcataga
     1261 tgatcgccac cttttggcga ggaacgcctg atgcaattaa tcgcccggcc tgcgcccatt
     1321 gttctggtgt aagtttggga cgacgtccac caattcgtcc ctgtgcgcga gctgcttcca
     1381 gtccagcttt tgttcgttca acaatcagtt cacgctccat ttcagccagg gcacccatca
     1441 catgaaagaa aaagcgcccc attggggtac tggtatcaat tgaatccgtc agactacgaa
     1501 agttgatgcc tcgttcgcgc aactcctcca ccagcacgac aagatgccgc atactgcgcc
     1561 ccagtcggtc cagtttccag accaccagcg tgtcacctgc cgataatgtc ctgagcagct
     1621 ttttcagtcc tggtctgtcg gactttgtac cgcttatctt gtcttcaaaa atcagctcac
     1681 atcctgcaca ctccagcgca ttacgctgta attccgtatt ctggttattt gttgatacgc
     1741 ggacatagcc aataagcatg agcattcccc tgagtaaaaa ccggggatga tgccagttag
     1801 ccataatctc tgcattttct taaacgttgg tttgggagaa gcggcaaaac gggatgtggg
     1861 gacaggggaa aatcagatac cggacatggc ctcttttgcc agtggtgatg gatggatgaa
     1921 attacccaac gggaaaatcc tgcaatatgg tcgtggtgcg gttacgccga cattatcgac
     1981 gcaaacaatg agaattacat tcagcatccc tttctccaaa aaagcggact gcgccatgct
     2041 tactcattct ggtgatggcg gtgcgccttt aggcgctggg cgagggttcg tgatgactgc
     2101 agaaggccca acgttaaccg gctttaattc tgcttacaga acgtcatcaa ccagcgacac
     2161 ggtatcgatg aattacagtt ggtgggctgt tggtgagtaa ttttattcag ggtgatttat
     2221 atgaatgaat atgtttatag cgcaaggcat aatgcttttt tccctgtgga tatgattgat
     2281 aaatataaat cagagggatg ggatttatca gacgctaagg aagtaaatca aaatattatc
     2341 agtgagttta tggctgaacc gccacaagga aaaatccgta ttgccggaga tgatgggctg
     2401 cctgcgtggg cagatattcc tccacccacg catgaagaac ttattgaaat tactgaatca
     2461 gaaagacagc tactaattaa ccaggccaac gaatacatga acagtaaaca atggcccggt
     2521 aaagccgcta ttggtcgtct gaaaggtgag gaactggcgc aatataattt gtggctggat
     2581 tatctggatg cactggagct ggttgatacc tccagtgcgc cagatattga atggcctacg
     2641 cctccggcag ttcaggccag atgacatccg gcgcggtgct ggtatctgtt gccgtcaccg
     2701 cgtcaatgta atccagcaca gcgttaagtc tggttgtttc tgcctgcgtc ggtttacgtc
     2761 cggcctgcaa tttcagttga atcagactga tggaagccat tgctgcatca atcagtgact
     2821 ggcgctgtgc ttctgccgct tctactgcgg caccgtgttg tgcctcagta tctgtcaccc
     2881 atttttcacc atcccattta tcgaatggcg ttaacggggc gatagtggtt gtattttcag
     2941 gataatcacc cggagctgtg atttctttgg cgtctcccgt ttcggtgtta tagacgattt
     3001 caccgcgatg gtctggcaca tattcccatg agtttaaatc catcgaacgg cagatagcat
     3061 aacccgcctt atgtatacca ggttcatcca gacaggaata tgcagggata ccgacaccaa
     3121 tggcaagata ttcatttgaa gtagaaatat attcccgagt tttaccatca tagttataga
     3181 cggtaatatt ccccgccttc gtggcaataa gctcgctatt taatacggcg ttatccatta
     3241 tgcagccctc acgatatagt taaatgcaat atttcgtgga cgggtttcac tcccgccagt
     3301 attaccgata ctccctcgtg aatgaagtgt cggtgatggg atcagactcc ctcctgtatt
     3361 tgtggcatca agtccccgtc cttgtgtgta tgtctttttg aaaatcgtag ccagttccca
     3421 ttcatctttt gtgtcgtaac catcattggc aacaacaata tggcggtgtt tttccagcat
     3481 ccctgtctga atgctcaata aaacacgtcc tgcatcaata ccgcgcccgt catcccagcc
     3541 acgaataaac tcaccacgta aatcaggcaa tttatttgtc ggataagcct ttgccagttc
     3601 cgggtattct tcagcagaaa aagccgcacc gttgcatttc agccagcctg ttggcggtgt
     3661 ggctgaaggc catggaacag gcacaccaac aggtaatgca gagccttctc ccaaaccaag
     3721 gttttcgaga gccgtttgca cagtgccatc cgatttgata tcgccaaacg gattcttgcg
     3781 gcttaacagc agcgcgcgaa gtgcggtaag taactggtcg tgccgcccct tctccaggct
     3841 ggcaccggat gcctccacca cgctgcaaag ttcctcctgc aacatgtcaa agtagtcatc
     3901 atccagatcg gtggcaggcg tgccggtctg ggggttacca cgggtaaaac cgttcttacc
     3961 cgcgccgaac ttatccttct gcgcggtttt cgtgtctata cgatgcatgg attactccgg
     4021 atatttaaaa attacgtagg tttgcgacgg gcagagtttg ttaagcacac actcgacaac
     4081 ggtgtcaccc cagatacgca gtgcggaatc acagggatcg ccacatgtca tccaggtgga
     4141 gttggtggtg gctggcatgt tgacctgcca gtaataccgc cattccggcg cattcaccgc
     4201 gtcagtacag gccgatgagc aggtgaaagt gcttttgtcg tatcgcgtga tggtggcatc
     4261 tggtctgccc agggcagcaa gctgtgcaag ataaaaatcc tcgttgatgc cgcccgccag
     4321 gttaaccttc gcatccagcc gttgctgacg ctggcgaagg gtctgcgttc ccgccggaat
     4381 acattcatcc ggcagaccgc acagacgctc ccagcggttt atcagttcag tggtggtgcg
     4441 aggatccagc tcccgcatca gggcatccgc acgctgatga acacgggtta atgacggtgc
     4501 cgcaccggca atcgccggat cgctggctga ccacgccgga ccgggcggca acagtgccga
     4561 taacaggcgg atgtaatcat cgtttgtcac gtccatgaaa tcgtccccag taccgccagt
     4621 tcgtttttcg caatggagat attgtccacc ggtgcaagca actgatggct gtattccccg
     4681 ttcgcaccgg aaatcgcctc actgatacgc gataccttga gttctccctg cggataacca
     4741 tcacgcagca ggaacgaacg caactccgcg gtaatggcag cccgtatttc cggtgtgtcc
     4801 ggcgtcacgc ggatatgaaa atccacttta tgcgccaccg gcctgaatac atacaaatca
     4861 gagcctgcca ccggggccag tggctcaatg tgttgtcttg ccgccgtttc cgttgattct
     4921 tccggaatgg gattaatcag gtcactgctg gcaatcatca caccgacagt tcccgttccc
     4981 atccagtgac ggtatgtcca tgcgcgggta atgccgggca cttctttagc ccagacaaca
     5041 tagtccccgt cagccccgcc ctgaggcgtc cagtaatacc gctcaatgac gcgggcgcgc
     5101 cacgtttcca gatcttcagt atcgaatccg ccagtcaggg tatctgcaac accggaagac
     5161 ggcagaccat tcaccggcgt gaccaggatt aatgccgtac cgtcgtcagc gttaccgacc
     5221 gcgcctgcac ttgagcaggc gatcggcacg cgcaggacac caccggagct ggttgcatca
     5281 gcagttgccg tgtactgaac caggtcatcg cgctgaatca cgctcccggc ggtcaccttc
     5341 aggccatcgc tgacaccttc ccagcgcata tacccgctgg cagccgtggc ccccttgcgc
     5401 ggacaccgtt tcatcgcagc atgtcgcgcc agccaggact catcgcacag gtcaggcagc
     5461 atgttcattg ccagataatc gatgtaaccg taaaccgtat gcagcgccgc cgcatacacc
     5521 tttgcccgca cgtcttcatc catgcgccgg agcgtgtcgc tgacgtccag cctggcgaat
     5581 aaatcgttac ggagcatact gatattttct gccagcgtcg ggcgctgaaa ttcactgtcc
     5641 gccatgcgtt atcgcactcc acagatcatc aaaagaaatc attaccggtc cgtcacgacg
     5701 ccagagggtg atactgttac ccagctcatt aatcccggtg cggcggatat ccagatcaat
     5761 acgggacacc acgccgtcat caatcatcca ttgcaggcat tcgcggatat acccccttac
     5821 tgtctgcacc agctgattgg tcagtttgct gcgctgaagc agccacagtc gtgagccgta
     5881 acggtcattc tgtaccgcag gccaggtatc cccccaccat cccatcggga cgtcggcgtt
     5941 gtcatcaggc tccgcccgcc gccaggtaaa cagggaaatc accacggcgc gggtcagcgg
     6001 atccagcggt gcgctggcgc aggtgcgttt accgttcacc gtcagccaca gttccatcat
     6061 gcctccatcg ctttatccgg tttgtcggtg ttactgccct gaccgttctc tctgtgacga
     6121 tgcccgttat aggcaagccg catcgctgac atggtagtgc cgctggagtc gcacaggtct
     6181 ttcacctgtc ctgtcacttc caggtccatt tcaaaacgtg ctttaggtga attgcgaaac
     6241 gtgatcgttt tacctgcacc gtccaccacg atcccctccc gggtcagcgt cacagactgc
     6301 ccctgatcgt catagacagc cacctcaccc gtctgcaacc ctttcaggcg gtagcgccgg
     6361 tccgacaccg taacaaccac cgcatgagaa cggtcgccat ccggaaacaa caccaccgct
     6421 tccgcgccgc tgtttgccct tgcggtaaaa ccgtagggtt caagatgttc aaccccggct
     6481 ttgggttcac cggcaatcag ggacacatcc acggtctgac atttcgtggc ggcactgatg
     6541 cttttcacca ctgcccgcgc aatcaggccg agaagttgtc gctgcatggc ttcaatcgtc
     6601 ctcatcagaa cgggtcctcc tgtactctgg cttttttctt tttccgcgcg ccgggggctt
     6661 cgggttcagg cagataagca tcaggcgggc cgacacggat ttccgtcagg gtgccgttct
     6721 ggtcctgagt aaacgtgact tccgagacaa gcagttcggt attgtcgaaa ccacagactg
     6781 gatcgaagac aatcacccgc tggttgggct gccacagcgt accgttaccc tgtcgccagc
     6841 cctgcaccac ataggtggtt tcatccgtcc gcgccgcccg ttgtcgggct tcaaagtcag
     6901 cacgcgcaat acagcctgcc cccgtggcct gccctgtctg cctgatatac atcggacggt
     6961 aacgggcaat aaatgcgtcc tctgtgcggg cccgcagcgc ggtggtggtg acctcaccga
     7021 aatcatcgtc gtttccggca cgctgccccg ccacctggta aacagaaaac cgctcccgga
     7081 tactcttctc cgtatcacag gaaaggatgt tttccccaag taccagcgcg gtatgtgccc
     7141 gcgttgagcc aataccgcca atcaccagcc tgccgtgcgg atcgtcgtaa gccagcgcct
     7201 gctgctgacc gagtattttg ttgataacct caatcaccgt ttcaccgtga tcaggctgaa
     7261 catcaggaat aacacccgac ggcgcaccgc tgttcaccac ctcaatgccg aaaggcgcag
     7321 caagcgcctg cgcaatctgc accagcgagc gtccgttaaa ctgtgtcggt tcggctgcac
     7381 agtcaatcag gtcagccgtc agactacgtc cggcaatacc ggtgctgacc gaacgggcat
     7441 cgtaacgaac gggagtcgcc tccacccagc cggtgatcac cagctcatca ccaatcagca
     7501 cttccacttt tgaaccgttt ttaatgcgcg gctgaagcgt ggtgataccc tcatctcccg
     7561 gccactggcg agtgatctcc acactgaaat cccgcgccag ccgttcaata ccggcaccga
     7621 tgcgcaccga tgtccagcca ttccactccc ggccatttac ccgtagcgtg acattgtcgt
     7681 tcattgcact ggcaccttca gagggatcac cggcacaaag ccgggatgcg taatggcatt
     7741 acgccggata atgtccgcgt cacgcgccgc gttatcaaac caggtcgccg ccagcaccag
     7801 cgcgggtaaa acctcatccg gcgtgcgctg aatgatccgt gcagactgtt caaggcgcgt
     7861 gttgatatcc gcattcagat ctgctttcac ccggcgcagc gccagaaaca gcgcatcact
     7921 ggttgtacgg gacaactcct tatcaattgc cgtattcagt gtgtcgcgaa tgtcggtcag
     7981 ttcttcccac gttggcaggt caaccgtgtt tttcaccgcc ggtgcattgt tcagtgccgg
     8041 atgcgtgaca gaaggccagc cggtgctctg cgcgggtgtt gttgactgcc ctactgtggc
     8101 attctgcatc accgcggaag ttgtgggcgc aggcaatcgt gtgacggcat acgccgcttc
     8161 gctgattgcg gtcgtacgaa gggtgctggc aaccacgtta cgctgctgcg tcgccgtggc
     8221 ggtagtttta ctgtccgttt tccagacgcc gcgcggttgc agatcgctgc cgaggctgac
     8281 accggaaagc gttttgatca tggtgaccag gtcgctggcg ttaccataaa ggcgtttccc
     8341 ggtacgccac attttctgca cctgctcaac gaaatttttg cctgacgatg gtggcggcag
     8401 aagtaccgag atatccccct gcaacagcct ggcggcatcc gatacggcag aatccaccac
     8461 tttcatcgca tcagaaacat acccaagcat tgtgctggca ttaccgacga cgtcgttctg
     8521 cacaaaatct gccacgccat cgatactgaa accactgaag ctgtcactga tgcagtcatc
     8581 cagtgcagaa caggatgaca tcagcgtctg cgccgtcgcc gcacctgaag tggggtaaga
     8641 gagttctccc gcttcgacaa acttcaggtc aaagcggaca atacgccctt cactcttcga
     8701 tgtgctgacc cgaacttccc cgtcaacaca gactttcagc tcaccgtaag tcggatggac
     8761 aagcgtgccg ggaccgggtt tattcagcgc gtcaatcagg cgatcgcgct ggtcaaagca
     8821 gtcatctccc accacataag ctgtgatgga cgggcggaaa gtgattttcc ccaggtcttc
     8881 ggtatagggt ttgtcgcggt tcgggtattc gtgcgtttcc acacgacgac cggttcccgc
     8941 actttcttct tcaaccttaa acggcacacc gcgaaatgac gcgtcctgaa gtctgtcttt
     9001 ccacgtcata taaactccgt acataaaaaa tcccaccgga gtgggactca ttaacagatt
     9061 aatttttcat tacctgccaa agcgcgtata gccaacatca tggctgacat caaaaccgct
     9121 ggatcgcgtt tccataaccc gcatacccgg aggcgaattc acaaaagaga ccttgatctc
     9181 accatcaact tttggcgcag aagctttgtt aatcatgaag ggattcgggc ctgtggcacc
     9241 ggaggcgttg tttgactgag ccggatctac cgcctgataa ggtgtgtatc cccgtgccgg
     9301 tattcccgtc ccataagcat cataagcacc cgcgccccac tgcgcagagt taatggcatc
     9361 gaccgtgtca ccggaactgt cggtaaacca ctcaataatt ggcttcagtt tgtcccacat
     9421 atcctgaaac cacttaacaa ccggtcccca gttattgatt accatcccca gcggcgacca
     9481 ggcaaaaacc ttcttcagaa gttcccagcc agcctcaaaa taaggaccaa tggtttccca
     9541 gagcttcttg aaataaggtc cgacaacatc ccagttagtg ataattaatc ccgcagccag
     9601 ggctatcgcc gtcgcaatca ttccaatcgg cgtcatcgac atgatcctgc tgacaatact
     9661 gatggcaccg ccaacgccca tcaatcccag tttcagaatc gcaagaccgg cagcaagccc
     9721 gacgacgccg cgaataaccc ggggattttc atccgcaaac ttcgtgaatt tttcccctaa
     9781 ctcccccagc cattgcgtga tatttttggc gtcaccagaa aatgcgccgc caatagccgc
     9841 aaggccgtta gttgcggtcc ccgtcattgc ctcccacagg ttggacagcg taccaagctg
     9901 ggcctgaaca cgtttattca ggctggcctg tttattcatc ttctgctgga tctgatcgta
     9961 gccatccttt cctttatcga ttagtgcatt gaccacctga agggtttcgg catcatcacc
    10021 aaatattgcc ttaagtacac ctgttcgctt aacgtcggtc agttttcgca gctttgccag
    10081 ttgcctgaac atgttatcaa gaccgccaaa acttcctttg ccgtcagtaa aatcgagctg
    10141 taccccgagt ttctggcggg ccatgatttt attgacgtcc ctgattttct taacgcttaa
    10201 tccggactgg ataacttttc gcagggcgtt acctgccgac tccccgttca tccccatctg
    10261 atccatcatg acgctgatgg gggcaaggct ctgtgcagcc tgaagaccgt ccttgttcac
    10321 catcttcaga acagagctgg ttttagtgaa gaaggacaac atgttggtat cgtcaacacc
    10381 cagataaaac gccttctgga tagtgtcgaa cagccccatc atgtcttctg acgccgttcc
    10441 ggtagcatcc tgcatctttg cagcaaactc agcagccgct tccggtgttt ttttcagttg
    10501 taccgcaaga taagctgtcg ctttacccac accgccaaga atgttttctg ccgggatccc
    10561 ctgacgcacc agcatctgca tcatgttctg gaaatcagcc gttgtaccgg gtagctggtt
    10621 acccaggcca atagccagtt tattgatgtc ctgaaagctc tttccaacct cgccgttcgc
    10681 atccatcatg gcgactttca gcccggtggc ggcgttttcc tgatcggcat aagatttcag
    10741 ggaaagcgtc agacccgctg ccagtccgcc accaagcgcc agcccaccct gtgacgcttc
    10801 ttccgcctgg cgtttaaatc cccggatttt cttttgcatt ttcgacagcg cgggagaaag
    10861 cctgtcgaca ccggtgatca acgccttaag ctcaaattcc gccatgtgtg cgtttctcct
    10921 gctctatcct gtttgcctga ctgaccagca agggaatttc actgatcggc atattcagca
    10981 attcgaaagg attaatgcgc cagtagctgg cgcagtcaaa gaagcgatca gtgaggtatt
    11041 cagccgtcag gcctggagga aaaaaccagc cacaagccac gccgctgcat tcaggtctgc
    11101 cggagacatc tggtcgacag agctttgcgg cactttcgcc agccgcacaa tgtatttcga
    11161 caccacatgc gccagaagtc tgactgactc atcctgattc atctggtagg gataccccag
    11221 ctcgcggaca tccttcccgg tgggttcatc aaactccagt acggagagtg tctcgccatg
    11281 agcagtaatc ggtttcttta actcaagctc tttcattact ggtaatcccc ttcttcaccg
    11341 tggaactcaa gatcaaccgt gccttcttcg gcattatggt tcgcttcgcc gtgcagccag
    11401 gcagacgaca gtacatagac ctgaccgttc gccagctcgg cagtgatggt catctcatca
    11461 gacgaggtga ttttgcttac cggaaaattc ttcggcacct tgaaggtccc tttgacataa
    11521 ggcgcacggt gagtttcctt gcggtccact gaaccgtcca ggccgatgat gtcatcgtta
    11581 accgttttgt tcatgggcac ctcaatgccg ccggtcagcg atagctgctg accgtcaatt
    11641 ttgaaataac aggttccccc gatacgggcc attatgcaga ctcctctgaa tactgaagac
    11701 ggaactgatt aaccacggca aagacacgca gctggttaac atagtcaggc gggaacagcg
    11761 tgttcaggcg gttcggatcg ctggcatcac gctccacaac caggtactgc ttaaacagtt
    11821 cgtagttttc cacgatcccc gcacgctcaa gctgacggta ggttgccagc agttcccctt
    11881 tgatcaccgc tggggtgaca atcgcctgac cgggaccaaa gcgggtaccg tcactggcaa
    11941 gcttgtgacg cccgtactta ctggtaatga cggatttcag tttgcgcagt acatacgcgc
    12001 tggtatgcag cgtctcactg tcgaggtaac tgttatccgc aaccccgtaa gcgtttttcc
    12061 tgtacgtggt gacatcacgc tgaatgcgta gtaccccgct ttcgacatac gccgttgcca
    12121 cgccatgaga cagcagggtc tgttgttcgg tcatcgtgaa ccgtttcccc ttcggcgcag
    12181 gcagcatacc caccagctca ccggtctgcg tgggacgtgc cggatcgttg cggataaaca
    12241 ccgctgcgcg ggcggtacgg cttgccgcca gctcgtcggc aggcgtctgg gtctcttttt
    12301 cgtaccccgc cagggtaatg tgctgctggt taaactggtc acctgcggtc accagttctg
    12361 acagcgtgcc gatctttgcc gtatgcacat gaccatacag ctgacgcgca tagctccagc
    12421 gaccgctggt atcgttcatc tcggtcacca gcgtgttaac ggaggccgtg tcgttgaacg
    12481 gcaggccgat ataatcaaac ggctcatccg ccattgcagc caccgcgccg gtgagaaccg
    12541 gagcacccgt tccggcggta cccgtcgcca cggcaatctg tacgcccgct ggcagcactt
    12601 cgcccccacc aaagccgtag taattgaggc tgacaggaat ttcattccca caaagcccct
    12661 tatgacgcgc ggtcagtgtg accacgcctg ccaaagatga agccgtaaac ggcagggtcg
    12721 gaacggcatt gatggcatcc tggatactgc tggcaatcat cgtgacgtta tcgccgttag
    12781 tcaccggtgc ctgcacgcgg gtacgtccta catacacatt caccgtgccg gtttcggttg
    12841 ccgccccggt caccgtcagc gtaactgttg ccgccgcacc tgtggattca ggaacggcaa
    12901 tcacatacag ctcgccaaac gggtcagtct ggcgataagc ctcgaccata cgcgccagct
    12961 gacttcccgc accacaaatc tggcgtgcat agtctgccga cgacatcagt accagactgt
    13021 tggcaacaat ctctgcaccg ttattggcat gaccaatcag cagcgatgct ccgctgtcct
    13081 gtgcagtatt cgccgcctgg ttatccattt ccgcataaaa caacggaacc agcgtattcg
    13141 acggaatggt gttaaagctt atcgtcatcg gtgttcacct ttttattcac gcgccggata
    13201 tcacccgctg cttcacggcg cagccagtag ttgttctcgt caacatttcg cccttcggcg
    13261 ggcaaaaggt cgccgcgggc agggtcaggc actgaccgcc ctttaacagg tttgacaaac
    13321 atgaggatcc tcaggaagga agggttattt cggtgtgatg ttcgatatcg ccgtcaggcc
    13381 cgttaccggg ctcgagataa tcaacatcaa tcgccagcgt ttgcagttca tccagactgt
    13441 tcagatcatc ctgctggcgg gtatcgtctt cagtcagctc gctgatgacc gaaaaatcga
    13501 actgataaat cagctcatga cgattcagat ccagcagcgt gccgccgtca taggtaatcg
    13561 ggttaccgca cgcttccggg ttccagccca gcaaggcctt aaagagcatc tgccggacat
    13621 cgtccaccac atcatacgaa gcaaactgac cgagctcatc acgcccgtta ctcagtatga
    13681 caaccacgga gaagccctct ttcagctcct gccagtagtc ggtctggctt ttgttttctc
    13741 ccggagagtc atcacccggt accacatacg ccgccgggag tctcagcttt ccgacctccg
    13801 gcagattttt gaactgtgcc gcgcctgcca cccggttttc aaaatacggg cagcgggcac
    13861 gcagcgcagc aataacaggc gtcagtttca tctgcgtcgt cgctcaggct tcagtgattt
    13921 acgtaattct cgcgccagaa aatagcgtgt ccagctgcgg ttcttttcaa gcgtttccac
    13981 cataaagtta ttacgtggag ccagccgcca gccgctgcca ccggatgcac cacgatgatg
    14041 actacgacga cgttttgctc ctccccggac accaaaaaac agaaacgccg gatagaagtc
    14101 accagagatc atccggttcc ccttcccgtt gcgctggtta ggggcaatgc gtgtcataaa
    14161 accggctcgc tttttactgg ctctcggcac catgtaacca atcgaacgag ccaggcgtcc
    14221 ggtctgataa ccggggtttt cacccggtgc cgaccgcgca cggcgcatca ccagccgacg
    14281 ggcatcacgc atatgacgct gcccaatcgt gacaaacgcc cgccggacac gggcgcggtt
    14341 aaagcgcatc tcggcgggct gctgaacatc aacgtgaaaa aagggagtcg ccattgctgc
    14401 ctccgtgact ctgcgtaaat tcgcccagtt ccgtacactc cagcagcaga aagcgccgcg
    14461 ccccgttcag atcgcgctga cgtttcaccc ggtacacact gtcatcacag accacctcat
    14521 aatcagcagt gatcccgcgg cggtagcgaa tggtgatgta atgggtgatg gcgtccccgg
    14581 tctgcgcggt ttcctgccag gtggtggcac tggtctggat aaccttcgcc catgcccgga
    14641 acgcaaccgg gtattgaggc tccacgccaa agttatccgc gggcatatcc acccgctggc
    14701 ggatcaggac gcgtttattc agttcgccgg ggtccggcag tatgtaggtt gcgctggtct
    14761 gcgcctgacg aattttcata gtggtataag gcgataagga gcaaccaacc agttaaaact
    14821 cattggcaac tccattttct caacgtctgt aaccgttgag cggttttcgt agaaatggct
    14881 gacaagtagc agaagtgcca gcttcacatc atcagatatc acaagcccat caggatcatc
    14941 cgcaggcctg tcatctgcgg ttgcatacaa cttacggtta aggaagtttt ccgtacgact
    15001 ctgagcggcc ttaccaagca gttcaagcaa ctcatcttca tcagagaaat catcatccag
    15061 acggagctga agcttaatct cttccatttt taacagcata aaacctcctg tgcccgccag
    15121 aacgcgggca caaaaaaacc gcattacgcg gcgtgctgta ttacgtaaaa agactaatca
    15181 accaccaacg ctacctttcc ccaccagcgc tttaatggca gaggtgtctt ccaggataca
    15241 gtcaaaacga tggaaggcca gaaaaccggt ctgatcatat tccgcgtaac gctcaaccag
    15301 acgtttaaga atcatgtatc gcacacgacg gataatgaag cgatcaaagt caccacagaa
    15361 catgaatttt ttacccgccc cgatatcatc aatttcctga tcaatgacat acggtacatt
    15421 caacactgaa gcaggtgcca caccaacaat atccggcaac cataaagggc gtccctgacc
    15481 gtcttccatc tcactgatca gtttcagcgt attatcgtta aacgccaggc ggaatttcgg
    15541 tccgcgacga tatgcaggat caatgctgtg tttcagagcc agaatttcct gccacttcac
    15601 cgtatttgcc gcggcagtct gtgttgtgcc ggtcacagat gcgaccagcc ctttgggttg
    15661 tttaggcgta ccagcaccag ttccctgaat cagataacgg gcttcaccac gaccaatacg
    15721 ttcagcaatg cgacgggcaa gataagcttc catatcgatc gcactgtcct gcagcaactc
    15781 attagacaca cgaattattt tcgatgtcat tttgagcgcc ccaaggcttc ccataccgaa
    15841 atcggtgtct tcttcaccgg cttcttcatt ttcgcccagc agaacaccaa cttcggaagt
    15901 accatcagct gttgcccact ccatagtgcg accgtcagaa gtggtaagaa tctgcgccac
    15961 actggcgatg ccaccgtagg atttcatctt ctcaacaact ttcgccagga atgtttctgg
    16021 tacggtatat ccgccctttt catcctgagc tacgccctgg gcacgaagtt cacgcaacgc
    16081 ctttcgttct tctgatgtca gctcactggc accgtgacgc atccacttat caaaaacctg
    16141 agctcgtttc tcatcctgtt gtggattgtt ttccggatca agattctgac gctgctcttc
    16201 ctcattgctt tcaatgtacg cctgatcctg acgacgcagt tcttcttcgc gtgcaattcg
    16261 ttcatcaagc gcttccagtt cggattttgc tttgttccac tcagtgcgct gctcttccgt
    16321 ccatgcgtta tcaccaattt tttcattcag ggcgcgcatg tcagttgcga tagtattacg
    16381 tttctgtttc agttcatgca gtttcatgat gtttccttta cgcgttaaga agggtcagga
    16441 cgcgttcacg cgccatacgt tgattaatgg ctttctgtag cgcgccgctg ttgcgcgcct
    16501 cctgccatgc tttcatggag cgaacagccg agtcagcctc ctgataggca ggatatgtca
    16561 caggactgac atccagcaga cgggaaaagc gggttatctc gcgaataaca accccgtcct
    16621 catcctgata ccactcctcg ccgtcacggg cgacacggaa agcaaaagat gactggttaa
    16681 tatctccacg ttgcatcggg gccagcacca gatcacgaat ggtctgtgtc tccggagcct
    16741 ggatgtcata gcgcaatccg cgctcatcaa ctgaaagatt cagcgtgcct gctgcactac
    16801 gcccaagaat aaaattagga tcgtggttaa acagtgcgcg tacatcatca ccaagcacat
    16861 cgtcaaaagc gccgggccgg atgatttcgc ggaatgaacc aaatatcagc tcagaacgac
    16921 agtcaaacac cgatccataa ccgataatgt gcgcagggtt atcgtcatgc cgctcagcac
    16981 gcacctcacc gctgtaacaa cggatttcac ggtcattcat tggtttttcc ctcatcattt
    17041 tttgggggct taaaatctcc tgccgggtta gcagcattca cgcttaccag catctcatcc
    17101 agcccttcaa ccggattcat atcctcgaat gcgcgggctt cattacggct catccatcca
    17161 tcggtaatag cgaagtgata gaattgcgcg cgctcctgcg gagttccgcg taaaagcccc
    17221 gtcagattga acctaacgta atacccggcg gctaactcag cgcgggtaaa caagcgacgg
    17281 ttaagctcct gctcccagtt cgttacccac ggcatcatcg tgtagcggac aaactgaatc
    17341 gcctgcgcag aaatattgga gaaggtggct ttttcgaggt cattaatcat gtgcgcagga
    17401 atattgaaaa taccggcaat cattgaacgg ttcagtttca tcatgtcaat gatctgagcg
    17461 tcaactggcg acacagtcag tgccttgtaa tccagatcgg ctggcagcag catggttttg
    17521 ttttcctggc tgcgtaacgc ctgcgatgcc ttctgccact gatctttaag ccagccccag
    17581 ctgtccttat tgagtccgct tttaacggat actatccccg ccggacgggc attaccgctg
    17641 aagaagcttt ctgtgtattt ctgaccgctc atccccatgc ctattgtttc ggcatgttgc
    17701 ataatcggac tcagccccat cttctgatta ttacccagcg cacggatgtg gatcatatcg
    17761 tcggggctga tcgcaaacgc cccatattcg ttgtacaaac cgtaggtata tcggccacca
    17821 gtattcatca gcgtcgtttc ccacggcata cagcaatcca gggatatgac ttcaccgcga
    17881 cgattacgtt tcacccaggt atacccattc ccccagccaa ggatgtgacg ttgcttcagt
    17941 tcgcgccatt tgtaactggt ttgccaggta ttgggctcat catgaaccag ataaaacgcc
    18001 ggatgatcgc gtgcgggctc aaccttccca ttgtgcctgc gcataacatg caacggcatc
    18061 tgggcaaggc tggaagacag gacatagata caggaataca ccgcagccag tttcatcgca
    18121 gtttcaggac tgacataaac gtctgtccgg aacagcccat cagtatcaac ggcatccccg
    18181 gttatcgggg tggaaggatt ctccagtgat ttacttctga acagagcatc aagcagcacg
    18241 cgtccccctt ctggccatag ccagtgcgcc caccagcagt aaaccgccgg acagcatcag
    18301 agccggagcc ataccaaact gcaggtaaaa cccgcacgta agcaggccaa aaccagccag
    18361 cccgataaca tcagcaatta gtgatttcat agaattaaga gagcatcgtc cggatcaaga
    18421 gatgagagga aatcgtcggg ttctttgagc attgcccgac cgatcgccat aatcagcgca
    18481 accgcaccat cgattttgtt ttccgcctgc tccttgacag gcttcaccac atcatcgtta
    18541 cccggaatgg ttttgccgac cacgttgccg atacaccagg tcatgatggg attgccgtca
    18601 tgatgaaagc gccccgattc aatcgccgct tccagctctt tcatcggatc ggacatgttg
    18661 gtgtagttct gaatgatggt gatggggttc aggtcttcat cagcaaggtc atgcgacagc
    18721 ccggtcgccc cgaaggggtc gatgggtgac tcactgaccg ggctgatttt gttcgccgct
    18781 ttggcctcct cgaggatgta ccgataatcc acctccgcac cagcggtaac agtcagaacg
    18841 cccatttcca cccatttctg aaagcgttcg gctgtccggc gatcttcatt tttctcgacg
    18901 ctgtacaccg tgtcatacgg tacccagaaa cgcggggcca cactgtagta atgcgtttta
    18961 ccgtcaatct cgcgggtata aagtcgcgcc atgctgttca tatccagctt acgcgccagg
    19021 tcaaaggcca gaatgcacgg ctgcccctcg aactgctcaa gggtcagtga tttatcctcg
    19081 cagctctgcc agctcaccag gttgaaatac gccgaacgcg ccgacaccca gatattgagg
    19141 tgttttgttt taaagacgtt tgccagacgg gcgttatttt tcgcacgctg ctgctgactt
    19201 aacaaaaatt cgcgataaac cgacacgcca atatttggat tggctttttc cagcacctgc
    19261 gggtcgatcc agtcgtcacc ttcatcaacg gtatagatga tcccgaacag ttcatcgttg
    19321 ggtaccgaac cgttgagcat ctcgataact tcccgccgct tgtcgtagca cggcccctca
    19381 atgttgtacc cggcggtggt gatggcccac atcagtggct gacgtcgcgc ccccatcccg
    19441 gtaagcatcg tggtataaag cgcatcggtg gcgtgctcgt gatattcatc caccaccgca
    19501 cagtggggtg atgaaccatc accagggtta ccgatcagcg gttcaaaccg cgcaccatcc
    19561 tccggacggt tcatgtttga ggcgttaacc tcaatcccga acgcttccgt cagcatgggt
    19621 gtgcgtttac acatcagtcg tgccggacga aagacttccc atgcctgttt ctccgtcgtg
    19681 gcaccggaat acacttccgc gccgaactcg ttatcacagg caaaacaata cagggcgaca
    19741 ccggcagaga ttgccgattt gccgttctta cgggggattt cggtatacac ctcccggaag
    19801 cggcgcagcc gggagccttt attgacccag ccaaacgcgc agcagatcac aaagagctgc
    19861 cacggctcca gcgtgatggg catccgtttg aatgcccact cacccttggt gtgcggcaac
    19921 agctgaataa atttcgcggc ccgttcagcc aggtccttgt cgaagcggta acgaaacgac
    19981 ttactttttt ccgccatcag gtcatcaaga tggcgctggc aggcctgaat cacaaactgg
    20041 caggccacaa tctttccgcg cacgacatca cgggcatact gatttgcagc atttacgttg
    20101 gggtaagatt tccggctcat gattcgataa ttttcagaaa cgggttagtg gctttcttct
    20161 gccccgccag gccaatcaga cgctggcggc tgctggggtc gagtccgagc attgcccccg
    20221 tactgctcat ctcggactcc tgttcttttt tggcggtcag ctccggattt ttgaccatgc
    20281 cgcccattgc accggtgatg gtgttgccct gtctggcaat atttttcacg gcacgtcgcc
    20341 agaactcata ggccacgcac caccgctcaa gcaccgcgag gtcagtcacg cacagcaggc
    20401 cctgaccgca gagttctttg gttgtcagtt gccacatgat cgtggcgaga gggagatctt
    20461 cttcagcgaa ccactccggt ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt
    20521 tgttcagggc tcgcttgccg gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc
    20581 gacgcccgga acgccccgcc gttccagcca tatgcggcac tcctggttaa atttcatttt
    20641 tcgcgggtat aaaaaaacga tggggcgggc agtccggaag acgtcaggtc acagggattt
    20701 gacccgcccc tcccctctgg cagtgggaac tggttcttac ttcagccgtt cacgggccgt
    20761 cttcgcctta tgacacggcc agcacaggct ctgcagattg ctgtcggcat cagtgccgcc
    20821 atgcgcttta gggatgatgt ggtcaacggt tttcgcctcg cgcaccacac cggcacgcaa
    20881 acacaactga cacaggcctt tgtcacgctt tagtacacgc ccccggataa catcccattt
    20941 cgaaccataa ccgcgctggt gtcgggactg gcctggcttg tattgcttcc agccctcgct
    21001 tttgtggctt tcgcaataac ctgacgggtc agtcgtggta tggcggcagc cgcgaactcg
    21061 gcaggctttt ggggttcgtg gtggcatatg tactccaatg aagaagctac cgacatagcc
    21121 tcctccattc gtcgtgaaac tattttcatc tacccagtaa tgaattcttt gaagagttgt
    21181 aatcaataca actcgctaat ggagaggctt gtctcacacg tgagacaagc ttcctgtttg
    21241 atttactgga cactatagaa ggacagaatg ccttcctcac tcgaatcaca tcaattaagg
    21301 aggttcaaca tgtttcattc cacaagtcat caggctgtaa ttatggcagc atcagtttgt
    21361 gccacagacc ttttccgctt cactttgagc ctgattcatt tctacctgac cggctcgcct
    21421 ctatcttttt aatccccgct ttatccaaat tgcattgcca gaatgccgac aacagactga
    21481 cattcaaatc ctgactacct ccaatagtct gaccgtacac ctatatagtt ttaattttca
    21541 tcaatccatt taactatcgt ttaattgttg tcacatagga ttctgccgtt tttaacaatg
    21601 caggataata agatgaaaaa aatgttattt tctgccgctc tggcaatgct tattacagga
    21661 tgtgctcaac agacgtttac tgttggaaac aaaccgacag cagtaacacc aaaggaaacc
    21721 atcacccatc atttcttcgt ttcgggaatt ggacaggaga aaactgttga tgcagccaaa
    21781 atttgtggcg gcgcagaaaa tgtggttaaa acagaaaccc agcaaacatt cgtaaatgga
    21841 ttgctcggtt ttattacttt aggcatttat actccgctgg aagcgcgggt gtattgctca
    21901 caataattgc atgagttgcc catcgatatg ggcagcgcta tctgcactgc tcattaatat
    21961 acttctgggt tccttccagt tgtttttgca tagtgatcag cctctctctg agggtgaaat
    22021 aatcccgttc agcggtgtct gccagtcggg gggaggctgc attatccacg ccggaggcgg
    22081 tggtggcttc acgcactgac tgacagactg ctttgatgtg caaccgacga cgaccagcgg
    22141 caacatcatc acgcagagca tcattttcag ctttcgcatc agctaactcc ttcgtgtatt
    22201 ttgcatcgag cgcagcaaca tcacgctgac gcatctgcat gtcagtaatt gtcgagttcg
    22261 ccagcttcag ttctctggca tttttgtcgc gctgggcttt gtaggtaatg gcgttatcgc
    22321 ggtaatgatt agcccatgac aagcaaacga tgatgcagat aaccagagcg gaaataatcg
    22381 cggttactct gctcatacct caatctctct gaccgttccg cctgcttctt tgaattttgc
    22441 aatcaggctg tcagccttat gctcgaactg accgtaacca gcgcccggca acgaagccca
    22501 gatattgctg caacggtcga tagcctgacg aatatcacca cgatcaatca tcggtaaagc
    22561 gccacgttct ttaatctgct gcaatgccac agcgtcctgg cttttggggg agaagtcttt
    22621 caaaccaagc tgtttacggt aagcatccca ccaacgtgaa agaagctggt aacgtccggc
    22681 ggctgttgat ttgagtttgg ggtttagcgt gacaagtttg cgagggtgat cggagtaatc
    22741 agtgaagagt tcaccaccga caataacgtc ataaccgtgg ttacgtgtcg gttgtcgtcc
    22801 gttatccgtt ccttctgacc aagccaccat atcaaggaaa gctttacgct gggaatttag
    22861 tacctgcata aattactcct tagagccacc aaatttgtta ccgattactc gcattgcagc
    22921 cccacgaata gcatcgacac cgatcagccc caccccacca ccaatggcaa cagaaagtga
    22981 tttaggccat ccgacatact caagagcgga tgcaaaggtc agcgtcagag cgccacatag
    23041 caaaatctcg agcgtttttc gcttccagcc accaccaccg ccaaaatagg cgatgcgcaa
    23101 accagccata acgatcgaca taatcactgc acccagcggt gtgtctccac gccaccagct
    23161 ctgaaacaac tccagccagt ccggccaggt atttgggtta tgaggcattt cgtcatctct
    23221 cacctcgcga tatttgcggg tgctgtgttg gaaataaaaa ggccacgcaa cgtggccacc
    23281 agaattattt ccccaccagt tcacttacct ctttcaccgt ctgattaaac cgctctgact
    23341 caagttaaac acctaacgcc cgacgcccca gcgccattgc tgcttttatt gtggaaccga
    23401 atcccataaa gaaatcagca accagatcac caggtcgact actggcattg attatttgcc
    23461 tgagcatatc cgccggtttc tcgcacggat gtttacccgg gtagaactga acgggcttat
    23521 gcgtcctgac atcggtataa ggcacggaga ctgatacgga gaaatagcgc cggtgagatt
    23581 taaactcatc cagcaattca gaagatttgc aattcagtga atcatgagat accaccaact
    23641 ggtggtagga ttgtctcaac tagtgagctt tatacattta agtgcgatgc acagataata
    23701 accacgccaa tggaatatca cacacaaaat tgatagaaca ctaacaataa gccacaaaat
    23761 aattcacaat ttatttttac agtggataag tagcaaagta gtgaatgtga ataacggcta
    23821 actaagatga aacaaaaagc cccacaatat gtggggctcc ttcgcgattt atagtaaaat
    23881 aatcgattct ctagctgaaa aaatctttaa aaattatcag caatacgcaa gttttttacc
    23941 gtcatccggt actagtttga tcactacacc aggattctta cgctctaaca tctgagtttg
    24001 actccatgta ataccaaagg tttccattgg agatgcattg ccagcaaaga gttcggcgac
    24061 gactttagct gcctcaatct ctatctgctc atgatgtttc ataaaaatcc ccttgttcag
    24121 cactaagctg tagttgcttc tttgaagcat aaaggcaaca aagtttgtta cctgaagatg
    24181 tataaagtaa agcgtagtac attttgtgtg tgctttgtgt ggatccagat tgcatcaact
    24241 aaaagaggtt ttcaatactt ttgtaatgat tctggagaaa ttaagaatct aactcacact
    24301 gattggttag gtaaaacgct cctaagcacc attgcgcaac cacctcgttc gtaatcagca
    24361 atgtactcaa aaccactttc agtatagtat cttattgccc catcaactgg actaactaaa
    24421 gctatcgaat caatagattc accctgaagg ttcagaaatc gtgcatatgc agagaagcaa
    24481 tccaaaacga taccaagcat ctgatggtct aggtcttcat gtgcatcatt acgtttttcc
    24541 atccagcaaa tatgcaccgc atttttcggc ttacagtaac acccaaatgc aaatccaata
    24601 ggctccccac gataataaac aactagtttt atcgggtgtt cttccatcat atccataaac
    24661 actcgcgact ttagtccggg atcccaccgc agtttattgt tagccttgag gaactccata
    24721 tcttttagta ccagctcgtc ggcatatacg agccctaacg acttatgagg gaagcgccct
    24781 tttaaatact ctgcaacatt tttaaatact gctgtttgga aggcttgaaa catcatgtcg
    24841 atccagacga gacgaaagtt taaattctac ctactcgccg aataccattt caatgccaca
    24901 catctaaaat tccgaaaaaa aacccgcaca tcagcgggtt ttctactttt tattaaagcc
    24961 ggacacacaa tgcctatcat tgagaaaata ttatccattt tttttgaaaa atgcaagcat
    25021 catgtcgcca ttttcgtcga aaatcattca tctcgtcact ttcctcaatt gtgtctcagc
    25081 atacgcttct tcctgccagc actttgtaac cagtttatca atgacatctg catatccttt
    25141 gtaccactga taatccgtca ggtctggtac cagcttctgg acatgaagcc gcgccagtgt
    25201 ggttggtaaa cggctaaacc ggtttccatt gcaacgccca caaaccttat aaacaggcgt
    25261 gccatgaagc ctggttcttt tttcatccag gacaatacct ttacccttac accctctgca
    25321 cgctgtgctg acttctccct taccatgaca atgctgacac agttccttca cccactcttc
    25381 cttgataaca gattccccgc ttctggagtg tttcaccacc tcgcgcaata catcatgaaa
    25441 tccagtacca gcacaatgct cacagcgagc cttacttgcc gcagacctgg aataatcagc
    25501 aaaggcaaaa ttcacaaggt aaggaatgat ctgtagccgg gtttcttcac tcaatttgtt
    25561 caatgtcggg ttatccagtg ccatcgcgta attgagcaga ccttcaatcg caaactgagg
    25621 atcctgaaca ccaacttttg ccaggaataa ggcaaaccca agcggtgctt tcgactgcac
    25681 catcccctgc gcagccatta catccgtaat tgttaaacca ccagagcctg tcgccggtgc
    25741 gtcatcgctc aattttggag attttgggga gtaatatttc ggtaaggctt caaagttcat
    25801 gctcgttctc cacttacgcc agtacgccta ttgccagcgc acgatcgata aaacgaaata
    25861 tcagctccag ctgggagcca tacttctctt caaatgccac ggtatccgca tgcagctcgt
    25921 cgtgatgctt tctgcacaaa ggcaacacaa agaggtcatg cgcttttgta cccattcctc
    25981 cctgaccgtg acctatcagg tggtggggat catcagcagg ctttccacaa catgcacacg
    26041 gctgtgtctt aacccagcgc gtgtactttt cattaaccca gcggcgacgt tttgggcgta
    26101 acataaaaga ctccggcgac tcaggatcca ctttcagcgc cagcaccttt ttcgctttat
    26161 cctggatgat gctggtggca ggaaccgaag gcacaaggtc actttcccgg gtaacagacg
    26221 gcacaacagg cttcggtaat ctaagtgcct tacgggctgc actttccggt aaggcatccg
    26281 ccaggtcatt acgaatcagc caccagcaca gttccggcat tgtcacaacg tgactgtcat
    26341 caaaaccgag atcccgacgc acaacagaca acacccagcg ggcacagtta tccgttgcca
    26401 ttgattccag ccgttccgtg aactgatcgc gcagctggtt atcgcagtgc cagcacagac
    26461 ggattgcgcc cggagcgtgt cgcattgtgg tcatgttctc gctgtgccag tcggaatgag
    26521 gccactggca gcctttttca cgaagtaacc agctttcaag acattccacg ccaccagcac
    26581 gacggatcac tgcctcattg cggaacacgg cccgaacggc aggatcatcc gccagcggtt
    26641 gtgatgccgc cggaacggca ccactggcga aagatgaata acgttccggc tcaggctcca
    26701 gcaggacacg cccctgcata aacaggggca tcagctctga acctggcctg aacaatacga
    26761 tccccatacg cggggcaatt tcaggagtca gtagtgctct cacggtcacc tcaatgaacg
    26821 gtatcgagca gctttaacag ctcagggaat cgggattcga agaaatgcgg ctgcgtctcg
    26881 cgcggatttg cgggactggt gatgttcttg ccgaacatgc agcctttcgc tgtcagcgac
    26941 cagaattttt tgatgttgtt aatcgcagtg cggctgtatc gttcacgttg ttcaacgatc
    27001 cccagcttcg ccatctggtg atatgcctga ttagctgtca ggcggatacc atactgcttc
    27061 agcagtgcac tcaatgatag cgtggggcgg cttgagccat caggcgcgtc agcaggagca
    27121 tcaatggcat agcgcggtgc cagattcggt aagccaacag cctcctggag tttctgacag
    27181 gcaccaagca ctgaagagtt agacaggttt aactcccggc gcataaagtc cagcaggatc
    27241 acgccagcct gcatcttgtc agcagcctgt ccggataatt tttccggtgc gctggttacc
    27301 atatcgaaag tacggatcac cttcagatgg aatgacgggc tgatccacat tgcataggca
    27361 tacaccagtt ccttgcagac atacgttccc cgttcatttc ccccatgaat cacactcacc
    27421 gggtcaacac ccaaattctg ggtgttggtc aattcatgaa caagctcaac agtttgttgg
    27481 ctggaaagaa actttcccgg ctccttggtt ctggcatttg caccagatgc tactgctgcg
    27541 cgatgcagat cgttcaggct gtaacgccca taagcatcac gacgaacttc aataccatca
    27601 atgaccatca gattattcat acttcgtttc tcctcttaat caggcggctg cacccgccgt
    27661 tttctcgtac ttactgatag tgatctcgac cttcccttcc gggataaccg gtccccactc
    27721 caccagcatt cttttcacct gactgtcgtc ttcccacaca cccgcgtggg tcagggcgtc
    27781 aaacagcgcc ttgttatagt tgtccagatc gcggatccgg ttatccggag gaaacaacac
    27841 gatctccact gaagcaggtg ccgacgttgg tttcggcaga cgacgtaact gctcaactat
    27901 tgctgcgcac gccgcgctct ggaattttcg ccccgccgcg cttatcaggc tcttaccagc
    27961 aaacgcccct ttgttggggt gtcgccagta cgtgttcacg ctgggcggaa aaggcaggat
    28021 cagcttcata ctttcaggtc cctctcatgt aaccagtggg ttgcacgcag ccttgcgttt
    28081 tcctcaccgg caagcagtgc gcggataatc ccgaccgcct cgctgtcgtc gtccttcacc
    28141 gcggtatgaa gcgttatccc ccgggccacg ccacgcttta tcgtgatgac gccttttttc
    28201 tccagtgcgc gaagatgctc caccgctgca ttcactgaac ggtatcccag catggttgcc
    28261 acctcctgat tggttggcgg gaagccacgt tctttctgat aagaaatcag catatccagc
    28321 acctgctgct ggcattgagt taacgtcgtc atgccgccat ctccctgacc agtttttctg
    28381 cctgctggcg aacctgcgcc agaaaggcct caccacatgc ctcaagttca tagcgcccga
    28441 tgtagctgat tgccggtccc ttccaggtct tgtcgaaaac agcaatagca ccagcgaaga
    28501 aagcgcctgt cggcacctgc ttctcatcct tcgggataaa ccaggcaggc agttcaaaac
    28561 caatacgccc gcgaataaaa gcaatatgat ctgcatcttc cggccaccac acttcgctgg
    28621 tggcagcttt gatcaggaaa acatagcgcc cgcctttatc acgcatggca ctggcatgct
    28681 tcatgatgta acgcatgccg gtgatgtatt gcccctcatg ctgactggcg cggctgtatg
    28741 ggggattacc aaaggcagca cctttaagct ccgcaagacg ttctgaccag tcatgcgcca
    28801 gcgcgttgtc ttccgcagtg taataagcgg cacatttggc gttatcaccg tcagtgaaca
    28861 gatccagaac aaacgggcca aacagggtgt taattcccca gaaaatgttg tccggcgtgc
    28921 gccactgatc gcccacttcc ttcagttcat gggctggttt gttccgcagc tccaccagcg
    28981 cctggcaata tttattactc attaagcccc cacgtaattc cctgacagat accactcttc
    29041 acccgataca gcgcgcttgc tgcttttccg taagcaccgc tcacgacgcg ccagaaaatt
    29101 gtttcgttct ggctgggagt ggctttcacg gaatgccgcc atccacaccg ttgcagcacg
    29161 acggtataag cccctggact ccagttcttc agcctggcgg gtcaggcaca aaatcacccg
    29221 gggatcgtta gtgccgacat agaaattgcg cacaggtctg gtttcacgaa ctggttgtgg
    29281 ttccggctcc tgcgctctct cagtcaggcg cgggaaatgt ctgcgtgtat ccccttcaca
    29341 acggtgagcc acacgcccac tctgacgtaa cttgcttgca gactgcagaa cgcgctgccg
    29401 tgagtaacct gcaaaagcat ccgcaatgtc tccggaagta cagcccggat gggcttcaat
    29461 gaatttctga acgtcattca aaagactcat gctcaccccc tgaatcctgc cgggatctgg
    29521 ctgtagtcca cgttgtcgta actggctttg aagtacgggt cctcgcgttt ttcggtgtac
    29581 gtgctgacgg acggcgataa gcgcagggaa agctcatccc atttttcccg cagcttcgac
    29641 gggctgagca cgttacggca ccagaacgga tcgcgactga cgcggctgta catctcgcag
    29701 atttgtttat gagtacgacc atcctgcaca cacatcaggc gaatttcgtt tgcccaggct
    29761 gtccagttcg gttctttggg acgaaccacc tcgccgtcac attcggcggc atgctcgtac
    29821 agggcgatga tttttttcca gagccactgt gcacaggtca aatcatcctg cgtcccccac
    29881 tggcgctttt tagggctgaa tacaaccgca tcaggatggc gagttaaaaa atcctgttca
    29941 tccgtctgcg tgtccggttg cgaagcgtcc ggacgagaag gttttttatc tgacggatca
    30001 tgttttgatt ttactgacgg atccccgcca gattctgacg ggtgaaaacc cgcttttttg
    30061 ccagatttcg acgcatcaaa ttttgacggg tcagattttg atgcgtcaga ttttgacggg
    30121 tcagaatctg acagttgaga aaatgccgct gcctgaagct tcgcaacgtt aagctgataa
    30181 acattcgacg cattgcggtt accctggcga cgcgccttac gcgttaacca gccttctgct
    30241 tccagccgtg cgatagccgt tctgacggtg ctcatccccg cgccaatctg gcgggcaatg
    30301 gtttcaattg atggccagca cacaccttcg tcattactga aatcagccag gcgggccata
    30361 attgccacgc tggataactt catgcctgac gcagcgcaac catcccatac atagccggtt
    30421 aatttagtgc tcatgaccga cctctatttc cctgaattta cgacgaaact gttcgagcgg
    30481 gctgaagcac tcatgctcat agccttcgcg gaggtagata acccgttgtg tttccggttc
    30541 ccaacgaatg actctgacgg gcactccgta gtgatctttg aaccagcggt taacttgtcg
    30601 caaaggactg tctccttttg cctgttgaaa tcacccacag cccactctgc aaagctgtgg
    30661 gttacaattt ccctgtcacc tggtacattc actgcatagc aatactccac cttcgctttt
    30721 ccacccggta caggaagtgc aatcagttgc gagcgacggt agtgtgttgt taaactgttc
    30781 atgcgttagt ttctccacag tcacgacacg ccacggcgcc cggagctgca cactcgcggg
    30841 cgtcattact ttctgaaatg caaaaaattt tgtagaccag tgctgcatgc tcctgcagct
    30901 tcgaaattga gaggtacagc tcgtcgttaa ttgctgtctt ctcatgcggt tccactacac
    30961 cgtcttcaat tgctgaacga atctgttttg aataactgcc gatctgttca atgacctcca
    31021 gcaggcgttg gttgatatcg gcgttgtcca catcctcgac atcaggaaga gacacaaaga
    31081 cgccatttgc agactgcgcc acagcgtcag caatgaagtg agttccacca gcacgttgca
    31141 aaatcattgc ccatcccagc gggaaaatct gatcgccatc ggcacgaagg cggttaaata
    31201 atgcgttctc tgttacatcc agccagtcag cagcttcagc gtaccccccc ggcaacgctg
    31261 cgatagtttt tctgacagct ttcacgtacc actcaggctg tttttctact ttccagtgat
    31321 gcttacccac ggttcacctc ctgttcctgt ggtttaaacc cattctggtt ttggctagat
    31381 tgaaaacgtg ccggataaag aatctgcatt tcgctgactt cacccttaaa aaaattggct
    31441 aaacgttctg caagctcgat agatggaatc tgctccagcc tctcaatacg actcaacgtc
    31501 gctggattga cttgaacacc cgcagcaaca tgctgcaaag tgaaaccatg cgccttacgc
    31561 acatttcgta atggtgattg catacgccct ccaaatattg cgcgttatgc atgttatttc
    31621 acgcaattat tttgcgcaag ttgatttgct tatcacgcaa taaagaaatg taataaacgc
    31681 atgaacatag gaaaccgagt cagacaactt cgccaagcga agaacatgaa aatcgccgat
    31741 ctcgctgaag cgataggagt agatgcggcg aacatctcgc gcttagaaac gggtaagcaa
    31801 aaacaattta ccgaacaaac actgagtaat attgccaaga gcttaggtgt tgatattgct
    31861 gatctcttta cctctgccca caaaagtaat actgtatata aaaacagtaa taatgaggat
    31921 gttgcgcagg tgaaggatgt gttccgtatt gaaatgctgg atatcagtgc cagtgcggga
    31981 aatggcctta tccagggcgg tgatgtcatt gatgtgattc atgccatcga atacagaact
    32041 gataatgctg tatcaatgtt cggcggacga ccagccaatc acatcaaagt tatcaacgtt
    32101 cgtggggaca gtatgtgtcc aaccattgag ccaggagatc tcatcttcgt tgatgtcagc
    32161 atcaatcagt ttgatggtga tggtatatat gtctttggtt ttgatgacaa aatatacgtt
    32221 aaaagacttc aaatgattcc tgacaaactg ctggtgattt ctgataacca gatttaccgt
    32281 gaatggggaa ttactagcga aaacgaacac cgattcatgg tctttggaaa ggtcttaatc
    32341 agtcagtcgc aaacccttaa gagacataat taacctcaat atcccatcca tcggccaccg
    32401 aaaggtggct ttttattacc tataaatttg catatctcgc aaataccact tgcatatctc
    32461 gcaatttaat tttatctttt attccagacc aactacagga ttacaacaaa atctggttgc
    32521 aacacggtgc atgtgtcgta agcagtcagt aaatgtcaaa aacgaacagg caggacgccc
    32581 acgaagtagc cgcctggggc atatgaagtc caggatgatt cgttgagtca tgttgtgcca
    32641 ctaggcactc atgttaaagc aggtgtatga aatgaaagtc cagattttaa acaataactg
    32701 tgaagtcgtt tggtcatacg acatagccgc ccctgtagat cagagcggcg atagctggac
    32761 caatgggaaa catcagatta tggctggagt tgtgttctct ttacgccgtg ctttagaaca
    32821 ggctgaagtc tttccatcag accctgaatg gaaatggcct ttttctattt gtccaaattc
    32881 ggagagtaca tttcagaaaa ttggtcagaa agtcgcactc gaagagcatc agccaactgt
    32941 ttcctgattt tttcaggtaa ctcgtcggca tcgcagaaac aacaacgctc gatcatgttg
    33001 aaagccgatt cgtagaactg tttttgctga gtgtcgctga gacaggaaaa gagcgacgtt
    33061 acgatgattt tattaattgc attatcaagt tctttttcat caaaagtcat ttgattttcc
    33121 ttttatgtat acgggcttaa aaggatacca ccgagcctga agtggtgaaa agacaggcac
    33181 ataacagcta agtattttca accagagaga atccttagcg ttgtggtgaa tgcggctcag
    33241 cgcacgcggg ttaaggttga ggctgacagt cgaccttctg tggataccca cccgcctggt
    33301 gtgcaacctt cgccaggcac cgggaggcac ccggcaccac aactttatgc tgtgtgtagt
    33361 cttagcggta ccagcttgta cccttgcttc cggctggtac cgtccttttt acaaaacaga
    33421 gaagagcatc accggacgac gggctcataa cccaatccat ccgggcggct gccaccgcag
    33481 gtgttcttct ctgttttgtg gagaaactaa ccgcccctac gggggcattt atggaaatgt
    33541 aattgactca ataatcgccg gacggtgagg gcttcctttt acccgaattc agcgcggtgc
    33601 agcgcatata cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt
    33661 attatgacag gataaataaa gacgacatcg ttattaacga tatcgcggtt tccctttcaa
    33721 atatctgtcg ctttgcagga catctttcac acttctacag tgtcgcccaa catgcggtgc
    33781 tttgcagcca gctggtgcca caggaatttg cttttgaagc tttaatgcat gatgcaacag
    33841 aagcatattg ccaggacatc cccgcaccac tgaaacgact tcttcctgac tataaacgga
    33901 tggaagaaaa aatagacgcc gtaatacgtg agaaatacgg gttacctcct gttatgagca
    33961 cgccagtgaa atatgccgat ctcattatgc tggcaaccga acgccgcgat ctcgggcttg
    34021 atgatggctc tttctggcct gtactggaag gtatcccggc aacagagatg ttcaaagtga
    34081 ttccacaggc accgggccat gcctacggga tgtttatgga acgttttaac gagttatcgg
    34141 agttacgcaa atgcgcatga atgttttcga aatggaaggg tttcttcgtg ggagatgtgt
    34201 accgcgagat ctgaaagtga atgaaacaga tgctgaatac ctggtgcgta aattcgatgc
    34261 gcttgaagct aaatgtgcag cacaggaaaa caaagtaata ccagtgtcaa ctgaactgcc
    34321 accagcaaat gaaagtgttt tgttattcga tgctaacgga gaaggctggc taattggctg
    34381 gcgttctctc tggtacacct ggggacaaaa agaaaccgga gaatggcagt ggacatttca
    34441 ggtcggggac cttgaaaacg tcaatatcac tcactgggca gtaatgccaa aagcaccgga
    34501 ggctggagca taatgaccac ttttaccgac aaagaactga ttaaagaaat taaagagcgt
    34561 atcagcagcc ttgacgtgcg agacgatatt gagcgccgtg cttatgaaat cgcactccta
    34621 tctctggaag tagaaccaga tgaacgcgaa gcttatgaat tattcatgga aaagcgtttc
    34681 ggtgacttag tagatcgtcg gagagcaaaa aacggcgata acgaatacat ggcatgggat
    34741 atgactctcg gttggatcgt ctggcagcaa cgagcaggta tccatttttc aacaatgtca
    34801 caacaagagg tgaaataatg gagccataca gcctcacact cgatgaggcc tgtcattttc
    34861 tcaagatatc cagaccgact gccattaact ggatacgcac agggcgtctt caggcaacac
    34921 gcaaagatcc cactaagaat aaatctcctt acctcacaac acgacaagcc tgcattgcgg
    34981 ctcttcagtc tccgctgcat actgtccagg tgagcgcggg tgatggcata acagaggaaa
    35041 gaaaatgtca ctcttccgca gaggtgaaat atggtacgcc agtttcacat tgccgaacgg
    35101 taaaagattt aaacagtctc ttggaacaaa ggacaaaagg caggcgacag aactccatga
    35161 caagctaaag gctgaagcat ggcgggtcag caaacttggt gaaatacctg atataacgtt
    35221 cgaggaagcg tgtgtcaggt ggcttgaaga gaaagcacat aaaaaatcgc tggacgatga
    35281 caaaagccgg atcggattct ggcttcaaca tttcgcagga atgcaactaa gagacattac
    35341 tgaatcaaaa atttattcag caatgcagaa aatgacgaac cggcgtcatg aggaaaactg
    35401 gaaactcagg gcagaagcat gcagaaaaaa agggaaacct gttccagaat acacgccaaa
    35461 accagcgtcc gttgcaacga aggctacgca tctttcattt ataaaggccc tactaagagc
    35521 cgcagagcgt gaatggaaaa tgctggataa ggcaccaatt attaaagtgc ctcaaccaaa
    35581 gaataaacgg atccgctggc tggagcccca tgaagcacaa aggctgattg atgaatgtcc
    35641 ggagccatta aagtctgttg ttgaatttgc actggcaaca ggcttaagac gctcgaacat
    35701 catcaacctt gaatggcaac aaatagatat gcagcgccgg gtggcatgga taaacccgga
    35761 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
    35821 gaaaaaacaa atcgggaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
    35881 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
    35941 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
    36001 ctgggcaagt tggctggttc aagccggagt cccgttgtca gtgttacagg aaatgggagg
    36061 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctt gcacctaatc accttaccga
    36121 acacgcacgg caaatagact cgatcctgaa cccatcggtc ccaaatttgt cccagtcaaa
    36181 aaataaggaa ggtactaatg atgtgtaact tattgatttt aatggtgccg ataataggag
    36241 tcgaacctac gaccttcgca ttacgaatgc gctgctctac caactgagct atatcggccc
    36301 tgaaaggaca tgttcacgaa cgtgaatcac ggtggacaag gttaaaacta accgggcgat
    36361 gcgtcaatgg ccttgtgaat caaacggcta cttttgcatc acccggtttt atttacgcac
    36421 gaatggtgta atcaccaatg ccgatccact tgtaagtggt cagtgcttcc agccccattg
    36481 ggccacgcgc gtggagtttt tgtgtgctta ccgccacttc cgcacccagt ccaaactggc
    36541 cgccgtcggt aaaacgcgta gaggcgttaa cgtaaacagc ggacgaatcc acttcgttaa
    36601 caaaacgctg ggcgttgcgc atatcgcggg tcaggatcgc atcggagtgt tgcgtgccgt
    36661 gttcacgaat atgggcgatg gcatcgtcaa gatcgctgac gattttgacg ttcaaatcta
    36721 atgacagaaa ctcatcgtca tactcttcgg ctttaaccgc caccaccttc gcagggcctg
    36781 tctgcaactg cgccagcgca gatgcatctg cgtgtaacgt cacgccgctt tccgccattt
    36841 gtttgcttaa tgcgggcagg aagctatagg cgatgttttt attcaccagc aacgtttcta
    36901 ccgtattaca tgtgctcgga cgctgagttt tcgcattgac gattactttc agggcttcag
    36961 caatctctgc actttcatca acataaatat ggcatacgcc tataccacct gtgatcaccg
    37021 ggatcgtcga ctgctcgcgg cacagtttat gcaaaccagc cccgccgcgc gggatcagca
    37081 tgtcgatgta tttatccata cgcagcattt cactgaccag cgcacggtca ggattatcaa
    37141 tcgcctgcac ggcacccgcc ggtaagccac aggatttcag ggcgtcctga atcaccacca
    37201 ccgttgccgc gttagtgcga caggtttctt tgccaccacg caggatcacc gcgttaccgg
    37261 ttttcaggca cagcgaagcg acatcaaccg tcacgttcgg gcgcgcttca taaatcacgc
    37321 caataacccc cagcggtacg cgacgacgct caagacgcag gccgctgtcc agtacgccgc
    37381 catcgattac ctgccccact ggatcggcga ggttgcacac ctgacgtaca tcgtcggcaa
    37441 tgcctttcag ccgtgcgggc gtcagtgcca gacggtcaag catcgcttcg ctaaggccat
    37501 tggctcgcgc gtcagcaaca tcctgggcgt tagcgttgag gatgatttcg ctttgtgctt
    37561 ccagttcatc ggcgattttt tccagcacgc gatttttttc gcggctggag agttgcgcta
    37621 atttatacga ggcttgcttc gcggcaatgc ccatttgttc cagcatcagc ctgctcctta
    37681 acgggtgatc atgtcatcac ggtgaacggc aaccgggccg tattcatatc ccagtattgc
    37741 atcaatttct tgcgagtggt gtccggcaat acggcgtaat gcatcgctgt tgtaacgact
    37801 gacgccgtgg gcgatatcgc gaccttcgag gttacaaatg cggatgactt caccacgcga
    37861 gaagttgcca gtcacgcttt taatgccttt cggcaacagg gagctgccgc gttcaagaat
    37921 ggcggcggtt gccccttcat ctaccgtgat ttcacccgca ggcggcgcac cgaaaatcca
    37981 gcgtttacgg ttttcaagcg gagtcgcctg ggcatggaac agcgtaccga cggaaatgcc
    38041 ttccatcaca tcaccaataa cgcccggctt gctgcccgcg gcaataatgg tgtcgatacc
    38101 cgcacggcaa gccacgtcag cggcctgcaa tttggtactc atgccgccag ttccgaggcc
    38161 tgaaacgctg tcaccagcaa tcgcgcgcag tgcgtcatca atgccgtaaa catctttaat
    38221 cagttctgcc tgcggattgc tgcgcgggtc agcggtatac aaaccttttt gatcggtcag
    38281 taacaacagt ttatcggcac ccgccagaat cgccgccagt gcagaaaggt tgtcgttatc
    38341 gccgacttta atctctgccg tagcgacagc atcgttctca ttgattaccg gaacgatatt
    38401 gttatcgagc aacgcacgca gggtgtcgcg ggcgttcagg aagcgttcac ggtcttccat
    38461 atcagcacga gtcagcagca tttgcccgac gtgaatgcca taaatcgaaa acagctgttc
    38521 ccacagttga atcagtcgac tctgccctac cgccgccagc agttgtttcg aggcgatagt
    38581 cgctggcagt tccgggtaac ccaggtgctc acgtccggcg gcgatcgcgc ccgacgtcac
    38641 aataacaatc cgatgcccgg cggcatgtaa ctgcgcgcac tggcgaacaa gttcaacgat
    38701 atgggcacgg ttcaggcggc gcgatccgcc tgttagcaca ctggtgccga gttttaccac
    38761 cagcgtctgg ctgtcactca tgattctctg ccattcaatt ttaggaaaaa tgatatcaaa
    38821 cgaacgtttt agcaggactg tcgtcggttg ccaaccatct gcgagcaaag catggcgttt
    38881 tgttgcgcgg gatcagcaag cctagcggca gttgttta
//
LOCUS       DAZFTN010000036        38379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFTN010000036 DAZFTN010000000
VERSION     DAZFTN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..545
                     /locus_tag="ACHKV2_002020"
     CDS             <1..545
                     /locus_tag="ACHKV2_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939903.1"
                     /translation="SNFTVGLTGDTEFLDSAIIGPVGNSIKCYYVSSLSGFYYTPAYV
                     FADVEFSMRQYIKCSHLTNKYGDDNLMYVFCKGGYEFDVNYLCVMASDLDNIKNTGDF
                     PKITEDSPKASSKRNEVIYSLIKLIPEMGDVDLDTESLPKIADLIDSIAASRGIEFPK
                     THWQTWQKYLGRESQQKRKK"
     gene            637..861
                     /locus_tag="ACHKV2_002021"
     CDS             637..861
                     /locus_tag="ACHKV2_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6939904.1"
                     /translation="MNQNTQFQSLSDRVIREDECRKLTGVCRTTRYELEKKGNFPSRR
                     NLGGRSVGWLLSEVMEWVKSRDRINSGKAA"
     gene            complement(996..1175)
                     /locus_tag="ACHKV2_002022"
     CDS             complement(996..1175)
                     /locus_tag="ACHKV2_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6939905.1"
                     /translation="MATKAVNGKSKKLEARVPHAIADAVENLKEEGESTGQFIVSALE
                     GEIKRRQRRKAKEQE"
     gene            1375..1578
                     /locus_tag="ACHKV2_002023"
     CDS             1375..1578
                     /locus_tag="ACHKV2_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6939906.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFLALSAGESQIIHVTAWTEREA
                     RSRCPSGCVAVFVARIRQGVCRA"
     gene            1571..1789
                     /locus_tag="ACHKV2_002024"
     CDS             1571..1789
                     /locus_tag="ACHKV2_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939907.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAVNMQAAIDSVISNSGKA
                     GLSHVIIAAVHELKGGGINEGNYITRSR"
     gene            1758..1949
                     /locus_tag="ACHKV2_002025"
     CDS             1758..1949
                     /locus_tag="ACHKV2_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939908.1"
                     /translation="MKEITLHEAAERAHQTEIICRLLEVYPDKLTDADISAISSLLAR
                     LSGNVSAWLIAEGEKYNEQ"
     gene            1939..2181
                     /locus_tag="ACHKV2_002026"
     CDS             1939..2181
                     /locus_tag="ACHKV2_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939909.1"
                     /translation="MNNNKLATIELRILANSAISLNNAANEVMNMWLQSLVNNERDES
                     TARLIFAVSELLDSSTSKIESISEKINGLSGHAIGE"
     gene            2187..2486
                     /locus_tag="ACHKV2_002027"
     CDS             2187..2486
                     /locus_tag="ACHKV2_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6939910.1"
                     /translation="MKLKYPGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFNRDFSLVQAAPRSKPTSKEKARANIQEIKKMLNVFRGKK"
     gene            2483..4231
                     /locus_tag="ACHKV2_002028"
     CDS             2483..4231
                     /locus_tag="ACHKV2_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFO6939911.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELYALQIVPDGKKRVRLYQAGELDLVETKKIGQKLAAAGVQDA
                     KFYPEGMHGQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQMLAGRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDKDLPEKVAAEIP
                     VIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVADFSKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDDCDAFL"
     gene            4582..4827
                     /locus_tag="ACHKV2_002029"
     CDS             4582..4827
                     /locus_tag="ACHKV2_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939912.1"
                     /translation="MTKMRRDRTQQKYKALDMTELSLKVAIKVIDRHTREGYAKENPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSTGE"
     gene            4958..5152
                     /locus_tag="ACHKV2_002030"
     CDS             4958..5152
                     /locus_tag="ACHKV2_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939913.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDVIRRMNEAGIYGRAATPT
                     IGHLARVLLRERLGIATGEA"
     gene            5156..5317
                     /locus_tag="ACHKV2_002031"
     CDS             5156..5317
                     /locus_tag="ACHKV2_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO6939914.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSNEVE
                     GLISLLGEQ"
     gene            5447..5935
                     /locus_tag="ACHKV2_002032"
     CDS             5447..5935
                     /locus_tag="ACHKV2_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6939915.1"
                     /translation="MALTTKKKRFADALSRGANPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAASVYSNRFPIPKPPTDL
                     KH"
     gene            5947..6108
                     /locus_tag="ACHKV2_002033"
     CDS             5947..6108
                     /locus_tag="ACHKV2_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939916.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQSTEIRLQAAAVLMPYFHETLNPC
                     FQEEEEDDL"
     gene            6098..7021
                     /locus_tag="ACHKV2_002034"
     CDS             6098..7021
                     /locus_tag="ACHKV2_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6939917.1"
                     /translation="MICKNMKGVSAVRAASVLASCKFSHADAIEMTKRNDPAVSKFLE
                     KSIVNRMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLHSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIEIFKKHIANGNTLSTSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGAV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            8000..8320
                     /gene="clpS"
                     /locus_tag="ACHKV2_002035"
     CDS             8000..8320
                     /gene="clpS"
                     /locus_tag="ACHKV2_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO6939918.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8351..10627
                     /gene="clpA"
                     /locus_tag="ACHKV2_002036"
     CDS             8351..10627
                     /gene="clpA"
                     /locus_tag="ACHKV2_002036"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO6939919.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10973..11060)
                     /locus_tag="ACHKV2_002037"
     tRNA            complement(10973..11060)
                     /locus_tag="ACHKV2_002037"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11024..11026),aa:Ser,seq:gga)
     gene            complement(11314..11532)
                     /gene="infA"
                     /locus_tag="ACHKV2_002038"
     CDS             complement(11314..11532)
                     /gene="infA"
                     /locus_tag="ACHKV2_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO6939920.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11817..12521)
                     /gene="aat"
                     /locus_tag="ACHKV2_002039"
     CDS             complement(11817..12521)
                     /gene="aat"
                     /locus_tag="ACHKV2_002039"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO6939921.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12563..14284)
                     /gene="cydC"
                     /locus_tag="ACHKV2_002040"
     CDS             complement(12563..14284)
                     /gene="cydC"
                     /locus_tag="ACHKV2_002040"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO6939922.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14285..16051)
                     /gene="cydD"
                     /locus_tag="ACHKV2_002041"
     CDS             complement(14285..16051)
                     /gene="cydD"
                     /locus_tag="ACHKV2_002041"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO6939923.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16174..17139)
                     /gene="trxB"
                     /locus_tag="ACHKV2_002042"
     CDS             complement(16174..17139)
                     /gene="trxB"
                     /locus_tag="ACHKV2_002042"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO6939924.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17684..18178
                     /gene="lrp"
                     /locus_tag="ACHKV2_002043"
     CDS             17684..18178
                     /gene="lrp"
                     /locus_tag="ACHKV2_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO6939925.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18313..22341
                     /gene="ftsK"
                     /locus_tag="ACHKV2_002044"
     CDS             18313..22341
                     /gene="ftsK"
                     /locus_tag="ACHKV2_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO6939926.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVASAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            22496..23107
                     /gene="lolA"
                     /locus_tag="ACHKV2_002045"
     CDS             22496..23107
                     /gene="lolA"
                     /locus_tag="ACHKV2_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO6939927.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            23118..24461
                     /gene="rarA"
                     /locus_tag="ACHKV2_002046"
                     /gene_synonym="mgsA"
     CDS             23118..24461
                     /gene="rarA"
                     /locus_tag="ACHKV2_002046"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO6939928.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24552..25844
                     /gene="serS"
                     /locus_tag="ACHKV2_002047"
     CDS             24552..25844
                     /gene="serS"
                     /locus_tag="ACHKV2_002047"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO6939929.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            26083..28527
                     /gene="dmsA"
                     /locus_tag="ACHKV2_002048"
     CDS             26083..28527
                     /gene="dmsA"
                     /locus_tag="ACHKV2_002048"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO6939930.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28538..29155
                     /gene="dmsB"
                     /locus_tag="ACHKV2_002049"
     CDS             28538..29155
                     /gene="dmsB"
                     /locus_tag="ACHKV2_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO6939931.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29157..30020
                     /gene="dmsC"
                     /locus_tag="ACHKV2_002050"
     CDS             29157..30020
                     /gene="dmsC"
                     /locus_tag="ACHKV2_002050"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO6939932.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(30056..30682)
                     /gene="ycaC"
                     /locus_tag="ACHKV2_002051"
     CDS             complement(30056..30682)
                     /gene="ycaC"
                     /locus_tag="ACHKV2_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO6939933.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30997..32145
                     /gene="ycaD"
                     /locus_tag="ACHKV2_002052"
     CDS             30997..32145
                     /gene="ycaD"
                     /locus_tag="ACHKV2_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6939934.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32355..33785
                     /gene="ycaM"
                     /locus_tag="ACHKV2_002053"
     CDS             32355..33785
                     /gene="ycaM"
                     /locus_tag="ACHKV2_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6939935.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33786..33896)
                     /locus_tag="ACHKV2_002054"
                     /pseudo
     CDS             complement(33786..33896)
                     /locus_tag="ACHKV2_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33995..34735)
                     /gene="pflA"
                     /locus_tag="ACHKV2_002055"
     CDS             complement(33995..34735)
                     /gene="pflA"
                     /locus_tag="ACHKV2_002055"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO6939936.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34927..37209)
                     /gene="pflB"
                     /locus_tag="ACHKV2_002056"
     CDS             complement(34927..37209)
                     /gene="pflB"
                     /locus_tag="ACHKV2_002056"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6939937.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37264..38121)
                     /gene="focA"
                     /locus_tag="ACHKV2_002057"
     CDS             complement(37264..38121)
                     /gene="focA"
                     /locus_tag="ACHKV2_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO6939938.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9952 a         9175 c        10030 g         9222 t
ORIGIN      
        1 gaagtaattt tacagttggt ttaactggtg acactgaatt tttagatagt gccattattg
       61 gtcctgtagg gaattctatc aaatgttatt atgttagcag cttatcaggg ttttattata
      121 ctccggcata tgtttttgct gatgtcgaat ttagcatgag acaatatata aaatgttctc
      181 atcttacgaa taagtatggt gatgataatc tcatgtatgt tttttgtaag ggtggttatg
      241 aatttgatgt taactatttg tgtgtgatgg ctagtgattt agataatata aaaaatacgg
      301 gagacttccc gaagataaca gaagatagcc caaaagcatc gtcaaaaagg aatgaggtta
      361 tttactcttt aattaaactt attccagaaa tgggtgatgt agacctggac acggaatcat
      421 tacctaaaat agcagattta attgattcta tagctgcatc cagagggata gaatttccaa
      481 aaacacattg gcagacatgg caaaagtatt tggggcgtga atctcaacaa aaacgtaaaa
      541 agtgatatca cgtgctggta gtgtgatatc acacaaacgg cgttttacta ccgcctagaa
      601 tcgtcctcgt tcggcaacgt tcgaggacgt ttttttatga atcaaaatac acaatttcaa
      661 tcactatcag atcgcgtaat ccgtgaggat gaatgccgta agttaacagg agtgtgccgc
      721 actacacgct acgaactgga gaaaaaaggg aatttcccgt cacgccgtaa tctgggtggt
      781 cgttcagtag gttggctgct ttccgaggtt atggaatggg ttaaaagccg ggatcgtatt
      841 aattcaggta aggcagcgta gggggaacat atgacacata aaacaaaggc gacctgtggc
      901 ggtcgccaat gggaaaacac taaacataag cccgaacatc atagcgattt gctgtctggt
      961 gggcaatgtg ctcagtgtgc tggcttgctg gtggactatt cctgctcttt ggccttgcgg
     1021 cgctggcggc gtttgatctc gccttcaagt gccgaaacga taaattgccc agtgctttcg
     1081 ccttcttcct ttaaattttc tacggcgtca gctattgcat gggggactcg tgcctcaagt
     1141 tttttggatt ttccattaac tgctttcgtt gccatatctg atttccttgt tattaggtgg
     1201 ctgacagtat acacacaaga aaatggaaaa acactattga cgtggctgac acctgttact
     1261 agtatagtgg ctgacacctt tattgaggta atcgatgaaa agacaaagcc cgcaggtgct
     1321 accaacacca acgggcttct aacaaccaac gatagcaaca gtatcgaggt agctatgaga
     1381 aatcatacca cacacccgca agggcgggac tcgcacaacc tgaataaata catctggcgt
     1441 tttctggccc tgagtgccgg agaatctcaa atcatccacg taaccgcctg gacggaacgc
     1501 gaagcgcgta gccgttgccc gtcaggttgt gttgctgtat ttgtcgcccg tattcgtcag
     1561 ggggtgtgcc gtgcttaaaa ccttccgcgt tttttcccgt gctgttaaca agcgcggcta
     1621 taccgttggc atcacgcaga acgtgaaggc cgttaatatg caagcggcga ttgattcagt
     1681 aatcagtaat tccggtaaag caggtttatc acatgtaatt attgcagcag tacatgaatt
     1741 aaaagggggg ggcattaatg aaggaaatta cattacacga agccgctgaa cgtgcgcacc
     1801 agacagaaat catttgccgc cttcttgagg tataccctga caaacttacg gatgccgata
     1861 tatccgcgat ttccagtctt ctggcgcgtc tttccggcaa tgtgtcggca tggttaattg
     1921 cggagggaga gaaatacaat gaacaataac aaactggcga ccattgaatt aagaatcctt
     1981 gccaacagcg caatatcttt aaacaacgca gcgaacgaag taatgaacat gtggcttcag
     2041 tcgctggtga ataatgaacg cgatgaatca accgcaagat taatatttgc cgtttctgaa
     2101 ttactggata gttcaaccag caaaatagag agcatatcag agaagattaa cggcttatca
     2161 ggtcacgcta tcggagaata aaaataatga aacttaaata tcctggctta actgccagcg
     2221 gcaaaactcg cactaaattc atgtgcgggg atatttaccg cgataagtac ggcggcacgg
     2281 taatgattaa gggcgtggag gaacggcgcg taacttaccg ccgtgaaggc tacgaatatg
     2341 attgcgttat gcctgtttat cagttcaacc gtgatttttc tctggtacag gcagcgcccc
     2401 gcagtaagcc aaccagcaag gagaaagcac gcgccaatat tcaggaaata aaaaagatgc
     2461 ttaacgtatt caggggtaag aaatgaaact ggcaccgaac gtaaaaaaac agccacgcgg
     2521 aataaaacac aaagacacag aggtgattat tttcgcgggt agtgatgcgt gggcacatgc
     2581 aaaacagtgg caggagcagg acggccccgc atccggcgat aatgtgcctc ctgtgtggct
     2641 tgggccaaat cagcttgccg aactttatgc actgcaaatt gttcctgatg gaaaaaaacg
     2701 cgtaaggctg taccaggcag gagaactgga cttggtggag accaaaaaga ttggtcagaa
     2761 gctggcggcg gcaggcgtac aggatgcaaa attttacccc gaaggaatgc acggtcagaa
     2821 gtgtgaaaac tggcggcgct atctgaatgc tgagcgtgaa aatattgccg cagggcttac
     2881 catgccggag cagaaaaata cgcaactggc acaaatggca gacagtgagc gcgcacagat
     2941 gcttgctggt cgatttgatg gcgtttgtgt acatcctgaa agtgaaatcg ttcacgtatg
     3001 gcgcggcggg gtatggtgtc cggtcagcac aatggaactt agccgcgaaa tggtggcgat
     3061 ctattcagag cacagggcca ctttcagcaa acgcgtgatc aataacgccg ttgaagcgtt
     3121 aaaagttatt gccgaaccaa tgggggagcc gtccggcgat ttgctgccgt tcgccaatgg
     3181 tgcacttgac ctgaaaacgg gggaattttc cccgcacacg ccggagaact ggatcaccac
     3241 gcacaacggc attgagtaca cgccaccagc accaggggag aacattcgcg acaacgcgcc
     3301 aaactttcat aaatggcttg agcacgcagc cggaaaagac ccgcgcaaga tgatgcgtat
     3361 atgtgccgcg ctgtacatga ttatggcgaa ccggtacgac tggcagatgt ttattgaggc
     3421 caccggagac ggcgggagcg gtaaaagtac attcacacac atagccagcc ttctggcagg
     3481 gaaacagaac accgtaagcg cggagatgac atcactcgat gatgcaggag ggcgcgcgca
     3541 ggttgtcggg agtcgtctta tcgtcctggc tgaccagccg aaatatacag gcgaaggaac
     3601 gggcatcaag aaaatcacgg gcggcgaccc cgtggaaatt aacccgaaat atgaaaagcg
     3661 tttcacggcg gtaatcaggg cggtggtgct ggcgaccaac aacaacccga tgatattcac
     3721 cgaacgggcc ggaggcgtgg cacgtcgtcg ggttattttt cgctttgaca atatcgttag
     3781 cgaggcagaa aaggacaagg atttgccgga gaaggtcgcg gctgaaatcc ctgtgattat
     3841 ccgccgcttg ctggctaatt ttgctgaccc tgaaaaggca cgggctttat tactggaaca
     3901 gcgcgacggt gatgaagcac tggcgataaa gcaacagaca gatccggtta ttgagttttg
     3961 ccagttcctg aattttctgg aggaagcgcg cggcctgatg atgggtggcg gtggcgattc
     4021 cgtgaagtac acgaccagaa acagccttta ccgcgtctat ctggcgttta tggcatacgc
     4081 aggaagaagc aagccgctaa acgtggctga tttcagcaag gccatgaagc cagcggcgaa
     4141 agtttacgga catgaatata ttacgcgaaa agttaaggga gtaacgcaga ccaacgcaat
     4201 tacaacggat gattgtgacg cgtttttata attttttgta aaagccctct accccatcta
     4261 cctgaatgaa ataaacgcat attattcaac atgataagtg ggtagaggga taggtagaag
     4321 gctaataaaa cccctctacc ccctctacca tgattaaggt cgtttgtgag gggcgggtag
     4381 aggatgggta gagggccacg aaaagtcctc tacccatctg aaagccgcgc cattactgac
     4441 ctgaaagatg attaggtaga agaggtagag gaggtgcacc acaacctaaa actttttaaa
     4501 cgaggggggt aaaaataaat gtgcatacat caaaatcact taacaaacat gccgaccgaa
     4561 aacatgaatc aggggcgaca aatgaccaaa atgcgcagag acagaacaca gcaaaaatat
     4621 aaagcgttag acatgacaga gctttcccta aaggtggcaa tcaaagttat agaccgccac
     4681 acacgggaag gatacgcgaa ggagaatccc gaactaataa gcgcattcat gaccacggcg
     4741 gcggcaaact ttgccacgct gacagagcgg gagattgcag aagcggaaca ggtaacaacc
     4801 atcaacgtta aaagcactgg agaataaccg aaggagtgag cacatagccg gagcaatccg
     4861 gctttttttc gatctctcat atcttaaaaa atggttgatt aatgataatc tatcgaaaat
     4921 actgattatg cgatgataag tatcaggagg aaacaaagtg gaagagataa agcaaagcgc
     4981 taccgtgatg attgatcacg agatcctaaa cgagcttaaa aaacttcaat tcgatgttat
     5041 tcgccggatg aatgaagctg gtatatacgg cagagccgcc acgccaacaa taggtcatct
     5101 tgcacgtgtg ctgttacgcg aaagactggg cattgcaaca ggtgaggcat aacaaatggc
     5161 tatcacagaa cgcgaggcaa gcatcattaa ggccattggc gaggaaacaa gagacaccat
     5221 agcgccattg atggaaaaga tcgcagagct tgagacacga ataaacaagc tgagtaatga
     5281 agttgaaggc ctgatctcgt tactcggtga gcaatgacat ggggggggag tgttccggcc
     5341 ggaaagtcga tcaccctgga caccgccccc cgtctcattc gtagaaaatt ttgcttttca
     5401 aaaaagttac acgaaaagtt acaccccctc aaaacgaggt ttttgcatgg cattaacgac
     5461 caaaaaaaag cggtttgctg atgcgttatc aaggggcgca aatcccacgg aagccgctat
     5521 acatgcggga tacagcgaaa agaccgcaag ggttaaaggc tggcagctat ccaacgatcc
     5581 tgaggtgaaa aagtatctta aggctgtaac ttctgatgta acttttcagg ttacaaaacc
     5641 gaaaaaagtt acaaagaaag ttaccgaaaa agttacaccg gaacgggcat caattaacac
     5701 cattgagatg atggataacg gcctacccga ccccatcaaa gcaatgggca ggatactgat
     5761 tgacaacatg gacacagatc caaaactggc tttagatgcc gcgtttaagc tggcccagtt
     5821 cacccaccgc aagatcggca gcattgggaa aaaagaggtt aaaaaactca atgccgctag
     5881 tgtctactca aatcggttcc ccatccctaa gccaccaaca gacttaaaac actgaggtag
     5941 cgaactatga gcgaatacat cacagaccct attgatgtac tcacggaaat catgaacgac
     6001 aagcagcagt cgaccgaaat acggttacag gccgccgctg ttctgatgcc atattttcac
     6061 gaaacactaa acccgtgctt ccaggaggag gaagaagatg atctgtaaaa acatgaaagg
     6121 cgtgtcagca gttcgcgccg ccagcgtgct ggcatcgtgt aaatttagcc atgctgacgc
     6181 tatagagatg acgaaaagaa atgatccggc agtatcgaaa tttcttgaaa aatccatcgt
     6241 caacagaatg actacggata acatgccgtc gctggtggca gagccgatag taagagagct
     6301 tggcgagtat attttttcgc agtctgtacc aggcaagcta atcagcagcg cgcttaatct
     6361 tccgtttggc gcaaaattgc attcaatatc aggcaagggt agtaaatggc ttaaagaagc
     6421 cgaagaaatc cctgtcaaag agggagaagt aaacgaaagt gcgctaaaat tgtgcaaact
     6481 tggcgctatt gtgatgctga ataacgagct aatcaaattt agcacccctg gtacaaattc
     6541 agcgatcaga gacttgatca catcggaatc ggtgaaagaa atagataaaa aatttctttc
     6601 cagtgatcag gaggtggctg gcgtatcccc tgagggcgtg cttaatggtg ctgatgaggc
     6661 cgaaaacttc atagagattt tcaaaaagca catagcgaac ggtaacacgc tttccacgtc
     6721 gtcgctaata ttgcccgtag aaaacgcctt acagcttact gatgctgagt tctggcagat
     6781 agagcttttg agaataccgg taattgtgag cgaacaggcg gatcgcatga tgctgataga
     6841 cgctcaaaag ctgattatta acgttgaagc cacggttata accccaactg atgaggcggt
     6901 aataaaaacc gaatcagggg cggttagcct tttccagaat gacgctacag cgttcagggc
     6961 aatcacctat tgcggatggc aaaaactcga taaggccgtc accgttctgg cgaaaagctg
     7021 atttttccag catctatccc gatatgggga tcaccatatc ggggaagcac ctgaatcatc
     7081 attaccgtga tcaagggtat gtaaagccca ccagcctgat taacgtttaa ccggaaaaaa
     7141 agccaggtat ccaatctcga tatggggatc accatatcgg gatacggcgg taactggttg
     7201 atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc gttgattctt
     7261 cccaatttcc acagactgcg gaaattggcg cggtaactta ctgagattaa agcaaagcgc
     7321 aaaattgcgt tggctggtgg gcctgatgcc gataactggt tggaattgtt ccatagtgca
     7381 aaattgcact atggcctaac ccattgatta tttcgaaaac ccccattggg ggatgttggg
     7441 aaagtaagac taacccattg attcttccac aatcctcaat ttgaggagat cggaaacatc
     7501 acccttcaaa ttctgaatgg tcataatgac cacgcagacc atcaaggcaa ccacaacgaa
     7561 catcgttacg gttggagctt ttaccccact ggggaaaagg tacgcacctg aatcatcatt
     7621 accgtgatca agggtatgta aaggccacca gcctgattaa catttaaccg gaaaaaaagc
     7681 caggtaggtg gggataccca tatcggggaa catccaccag cttcataacg gatggccgga
     7741 aaatgataac ggttaaagcg tgttgatctt tgttcgtggt tgttcgatat tgttcgtgaa
     7801 acggtgtagt taatggtgta gttaatttcg ctgtttttga cggagttgtt atttgtaaca
     7861 tagtgaaaaa taaagactaa tcaatgaaat gttataaatg atagttatct atcatgtgga
     7921 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
     7981 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
     8041 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
     8101 tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
     8161 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg
     8221 agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
     8281 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
     8341 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
     8401 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
     8461 cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga
     8521 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
     8581 cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc
     8641 ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc gctatcttta gcgaacagga
     8701 gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt
     8761 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
     8821 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
     8881 tcagcttgcg cgcgtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg
     8941 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctagtgg gggaatctgg
     9001 tgtcggtaaa accgcgattg cagaaggtct tgcctggcga attgttcagg gcgatgtgcc
     9061 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
     9121 aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga
     9181 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc
     9241 tggtggtcag gttgatgcgg ctaacctaat caaaccgttg ctctccagcg gtaaaattcg
     9301 cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct
     9361 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa ctgttcaaat
     9421 catcaatggc ctgaaaccga agtatgaagc acaccacgac gtgcgttata ccgcaaaagc
     9481 ggtgcgtgcg gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa
     9541 agccattgat gttatcgacg aagcgggcgc tcgcgcacgt ctgatgccgg taagcaaacg
     9601 caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg cacgcattcc
     9661 agagaagagt gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa
     9721 aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
     9781 gcgtgcaggt ttaggtcacg aacataaacc ggtcggttcg ttcctgtttg ccggccctac
     9841 cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gctttgggca ttgagcttct
     9901 gcgctttgat atgtccgagt atatggaacg ccatactgtc agccgtctta ttggtgcgcc
     9961 tccgggatac gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc
    10021 acatgcggtg ctgctgctgg acgaaatcga gaaagcgcac ccggacgtgt tcaatattct
    10081 gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
    10141 taacgtggta ctggtgatga ccactaacgc cggggtacgg gaaactgagc gcaaatccat
    10201 tggtcttatc caccaggata acagcaccga tgcgatggaa gagatcaaga agatctttac
    10261 accggaattc cgtaaccgtc tcgacaacat tatctggttc gatcatctgt caactgacgt
    10321 gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg
    10381 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
    10441 ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
    10501 caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa
    10561 agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc
    10621 gcattaatct gattgtcagg taggttggtg aagtccgtaa tctcgaaaga ggttacggac
    10681 tttttattta tggggtggaa cggtgaaaac cctatttttg gaggtgaagg taatttgttg
    10741 ataattagtg ctgctggaaa aggtaaggat aaaaaagggt gacagcagga aaatgagatg
    10801 gttttgcttt attaacaacg ggctaaacgt gtagtatttg agttcactgc cgtacaggca
    10861 gcttagaaat tcactggtaa catactccac ccgcccacca tgttcactgc cgtacaggca
    10921 gataaaatgc gaaaaaaaag cccgtacttt cgtacgagct cttctttaaa tatggcggtg
    10981 agggggggat tcgaaccccc gatacgttgc cgtatacaca ctttccaggc gtgctccttc
    11041 agccactcgg acacctcacc aaattgtttt gctgccaaac ctcatgggtg gcaacggggc
    11101 gctactatag ggagttggag taaaacggtc aagaagaatt ttaatgataa ttattgtttg
    11161 ctcatactgt aaacaacttg tgcagtatat ctacatcgag acaagttatg gacttatact
    11221 tccaaagtac ttcatgcata tcacaaaata aaaaggccgg ttaaaccgac cttttactcg
    11281 ttctttctct tcgcccatca ggcggtaaaa caatcagcga ctacggaaga caatgcggcc
    11341 tttgctcagg tcgtacgggg tcagttcaac agtcactttg tcgcccgtca ggatgcggat
    11401 gtagtttttg cgcattttac cggagatgtg tgcagtaacc acgtgaccgt tttctaactc
    11461 tacgcggaac atggtattag gcaacgtttc aagaacggta ccttgcattt caatattgtc
    11521 ttctttggcc atctaatcct ctggggtatc actaccgtaa tttgaaccgg caagataatg
    11581 ccgaagttct gtaaataagt aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac
    11641 attactccga aaacacacgg ctaagccgca ccaaaagcgc aacgtataag ggagcggtga
    11701 gataaacgat gggcgttacc tgacgcgaaa aattccttat cggcagcggg gtaatgagcg
    11761 taaccaactc tgcgaccgca attataacac tctggggaga aatgtgccga aaacattcat
    11821 tcttgtggtg aaaacaagca tcgtggtacc cagaaattat tcggcaatcg tccgaggcgc
    11881 atttgattga gataattaag gtaattccgg cggggaattt cgcaggcacc aagcgatgct
    11941 gtgtgatcgt taaggacctg gcagtcgata agcttaccgc catgaccgat aaattcctca
    12001 cagaatacca gaagcgccgt tttagacgca ttttccatcc ggctgaacat ggactcgcca
    12061 caaaatagcg ttccctgggc cacgccgtac ataccgccga caagctcatc ttcacgccag
    12121 acttcaatgg agtgggcatg accgagttcg tgaaggcgat ggtaggcttc gaccacgcca
    12181 cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc cttcaatgac ctgaccaaaa
    12241 gcgtaattca tcgtgacacg atagggcgag cgtttatgaa atcgcttcat actacggctg
    12301 atatgcagtg attctggcca tagcaccgcg cggggatcgg gcgaccacca gaggatgggg
    12361 tcgcctggag aaaaccacgg aaaaatacca cgctggtaag ccattaacag gcgcgcaggg
    12421 ctaagatcgc ccccaagtgc cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa
    12481 gggaaggcta ttgaatggcg agaaagctga accaggcgca tgaccgcaaa actccacgca
    12541 agtcggatcg ttcaataata gcttacaaac cctgcttgaa ctggtaataa cgcccctgtc
    12601 tggcaagcag ttctgcgtga gtaccttgct caataatttg cccgttgtcc atcactatta
    12661 tttgttggaa acgagagagt ccgcgaagtc gatgggtgac cattaacacc gttttctcac
    12721 gcatcatttc tgcaagcaat tcaaggatct ggctttcggt tgtggcatct aagccttcgg
    12781 taggttcatc cagcaacacc agtggcgcat catgtaacag cgcacgggcg atagccagac
    12841 ggcgcagttc accaccggag agctggcgtc cgccttcacc taaccaactg ttgagacctg
    12901 catcctcgag ctgcttctcc agaccaacgc gacgtaagat caccgccaga gcctcatcac
    12961 tactgccagg cgaggcgagt aacagattat cacgcagcgt ggcgctaaac agatgcactc
    13021 gctgaggaac aacgctgatg gtctgtcgta gagccgcttc attcaggctg gctatggggc
    13081 tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc gcgggtcagc agttgtaaca
    13141 gtgttgattt gccgcatccg gttcgcccga gaatcgctat atgttccccg gcgtttacct
    13201 gaagagaaat ccctttaagt gcctgttggg attgctccgg ataagtgaac tgaacatccc
    13261 gtaatgtcag cgaaacgcga tcggcaacac gagtttgggt atcagggaag gtgacctccg
    13321 gtttttgatc cgttaagtca gagatacgta cggcagaggc aatgacttgc cccagatgct
    13381 gaaatgcacc cgttactggt gccagtgctt caaacgcggc taacgcgcaa aagacaaaca
    13441 gggcaattaa cgcgccgggt tgagcattgc cgccaacgcc gccagacgcc atccacagca
    13501 tcaggatcac cgctaacgcg ccaatgagca gcattatcgc ttgcgacaat gcggtcagtt
    13561 cagattgacg gcgttgcgct tccagccatt gaatttctgt attctctagt tgcgtgcgat
    13621 aacgatcgct ggcaccaaaa atggtcagct cagcttgccc ttgcagccag gccgtcagtt
    13681 gttggcgata ctgtccgcga agatgagtca gattttgccc ggtgcttttt cccgcacgat
    13741 aaaacagcgg tggcatcagg aaaagcgtca gtaacataat gccgcccagc gtaaaggcga
    13801 gggtggaatc aaggaaactt aacccgattg tcaccaccat aatcaccaca aaagcgccca
    13861 ccagcggcga gataacgcgc aggtaaagat gatcgagcgt atcaacatcc gccaccacgc
    13921 ggttgagcaa ttcgccctga cgatagcgcg ccagtccggc aggggagagg ggcaacaatt
    13981 tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa agtcgcgtcg tgacttacca
    14041 gacgttcaaa ataacgcccg gcagtacggg tgattgctgc accacgcacg cccgcagcgg
    14101 gtagcatata gttgaagctg tacagtccgg caacccccgc aaccgctgag gccgagagga
    14161 accagccgga aagtgtcaac agaccgatac tggcgagcag cgtcacaatt gccagcacaa
    14221 taccaagact taacatccat ttatgacgtt tatacagtgc cagatagggt agcaaagcgc
    14281 gcatttaaat ctcctcctga cgatgggcca gtaatgtggc gaatgggcca ccagccacac
    14341 ttaattccgc gtaacgtcct tgctcaataa tccggccatc ctgcataacc caaatgacat
    14401 cccagtcagc aagatcttct aactggtggg tgaccattaa cgttgtctgg cgcagagagg
    14461 cggcattcag cgcctccatt acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt
    14521 catccaacag taatagcgaa cagggattta gtaacgcacg ggccaccgcc acgcgctgcg
    14581 cctgccccac ggaaaggcgg gcagcctggt cgccaacagg cgtatcaacg ccttgtggca
    14641 ggagcggtag aaactcgctg acccaggcgt tatccagcgc tgcttgtaat tcttgttcgc
    14701 tggcatcagg tcgcgccagt agtacgttat cccgtaatgt tgctgccggt aattgtgggt
    14761 tttgaccaac ccaggagaga tgtttacgcc atgattctgg tgataaatcg cgtaattcta
    14821 tcccgttgat tcgtaatgat ccctgatatg agagaaaacc agaaagcgcg ttcagcagtg
    14881 agcttttacc tgaaccgctg cgaccaacca acactgcacg ttggcctgct ggcaaagtaa
    14941 agttcagcgg tccggccagc gttttacctt ccggcgacgt gataaacaga tcctcggcct
    15001 caatggtcag cggatcggtt gatgctaatt ccgtctcacc gcgctgcgga tgggcgagcg
    15061 gggtttccat aaacgttttc agactgtcag ctgcaccaac agcctgggct ttagcatgat
    15121 aaaacgtacc gagatcgcgt aatggctgga aaaactctgg cgcaaggatc agggccagaa
    15181 aacccgcagc cagcgtcacg ccagtatcgt agtgaccaaa atccagctcg ccgagatagg
    15241 aaaaaccaaa gtagaccgcc accagagcaa ttgacagcga ggtaaaaaat tcgagaatgc
    15301 cggaggataa aaacgccagc cgtagcactt ccattgtccg ttggcggaaa tcttccgaag
    15361 cagaacgaat actttcaatt tcagcttcac cacgaccaaa aatacgcaat gtttccatgc
    15421 cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag agcgagaaag ttacgtcggt
    15481 tagcatcggc agcccccatc ccaaccagcg ccataaacaa cggaattaac ggtgcagtgc
    15541 ccagcagaat gagcgccgca gcccagttag aggggaagat cgccaccaca atcagcaacg
    15601 gcaccgacac tgccagcgcc atttgcggca ggtagcgtgc atagtaatca tgcatatcgt
    15661 caatttgctc gagtaccagc gtcgcccagc tccccgcagg tttaccctga atccacgctg
    15721 gccctgcttg ttgcagacgg tcgagaacct gacggcggat ggcaaagcgg atatgctgcc
    15781 cggcgtgata acccacccgt tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca
    15841 gaaccagtaa cgtaaaggga agcagcaggg cttcacgggg aatattctcc ataatcatat
    15901 gttgcagaat tcgcgccatg aaccatgcct gggcaatgat caatatgccg ctcacaaagc
    15961 ccagcagacg agaaatattc agccaacgtt gggagatgac gctttgctgt tttaaccagc
    16021 gggttaactc tttttgacga gatttattca ttgcacgctt agcaggtgag ttatcagaat
    16081 tatttgcaga gcaatgttac aacggggaaa aaataaaggc gacccatagt cgcatggtgt
    16141 cgccttcttt acttttgtta ctgatttgta aaattatttt gcgtcagcta aaccatcgag
    16201 gtagcgttcc gcatcaagtg ctgccatgca gcctgtacca gcagatgtaa tggcctggcg
    16261 ataaatgtga tccatcacgt cgcctgcggc aaagacgcca ggaatgctgg tctgggtggc
    16321 attaccatga atacccgact gcactttgat gtagccgttt tccagttcca gctgcccttc
    16381 gaaaatggca gtattcgggc tgtgaccgat agcaacaaac agaccggcaa cgtcgagtga
    16441 ctcgatgtta tcgctgtttt gcgtatcgcg cagacgaacg ccagtgacac ccatttgatc
    16501 gccggtcact tcttccagcg tacggttggt gtgcagaatg atgttgccgt tctccacttt
    16561 atccatcaga cgcttaatga ggattttttc cgcgcggaaa ccgtcacggc ggtgaatcag
    16621 atgcacttcc gaagcgatgt tagacagata cagcgcctct tcaaccgcgg tattgccgcc
    16681 gccgatgacc gcaactttct ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga
    16741 aactccacgg cctttaaagg cttcttcaga gggcaggccg agatagcgtg cagaagctcc
    16801 ggtggcaata atcagcgcgt cgcaagtgta ttcgccgtta tcgccattca gacggaacgg
    16861 acggttttgc agatccacct tgttgatatg atcaaaaatg atctcagttt caaacttggt
    16921 ggcatgttcg tgcatgcgct ccattaataa cggaccggtc agatcgtttg gatcgccagg
    16981 ccagttttcc acttccgtgg tggtggtcag ttggccgcct ttttccatgc cggtaatcag
    17041 cacaggttgc aggttggcgc gcgccgcata gacagcagcg gtgtatcccg ccgggcctga
    17101 acccaggata agcagtttac tgtgtttggt cgtgcccatg agatccccat agttgttggc
    17161 agacaatggg caggattgta gggaatttac agacgtaaaa aaagagtatg acgattttgt
    17221 taacaatttg tgcaatcggc agcatcgata agcaggtcaa attctcccgt cattatcacc
    17281 tctgctactt aaatttcccg ctttataagc cgattaaatg atgaataaac gcccctgtta
    17341 atgaatatct ggcatgttgt actaaaaatc gatgttttgc tttgacaatc ccctggtgtt
    17401 ttgcgaaaac attcgaggaa gaaaaaaaac agtattctta tatgcgcata accatgcatg
    17461 taaataccat gtttaccgtg ctagtgaaat ctacgtatgg cgtggacaga cgccattcgt
    17521 gatgtcgata gctgccacaa ggcaacggtc ttctcaccgt agacccaggc attgcgcgcc
    17581 gtgaatcttc atgatttcgg tctatcgtga cgggtagcga ctctgaacag tgatgtttca
    17641 gggtcagaca ggagtaggga aggaatacag agagacaata ataatggtag atagcaagaa
    17701 gcgccctggc aaagatctcg accgtatcga tcgtaacatt cttaatgagt tgcaaaagga
    17761 tgggcgtatt tctaacgtcg agctttctaa acgtgtggga ctttccccaa cgccgtgcct
    17821 tgagcgtgtg cgtcggctgg aaagacaagg gtttattcag ggctatacgg cgctgcttaa
    17881 cccccattat ctggatgcat cacttctggt attcgttgag attactctga atcgtggcgc
    17941 accggatgtg tttgaacaat tcaataccgc tgtacaaaaa cttgaagaaa ttcaggagtg
    18001 tcatttagta tctggtgatt tcgactacct gttgaaaaca cgcgtgccgg atatgtcagc
    18061 ctaccgtaag ttgctggggg aaaccctgct gcgtctgcct ggcgtcaatg acacacggac
    18121 atacgttgtt atggaagaag tcaagcagag taatcgtctg gttattaaga cgcgctaaca
    18181 cggaacaggt gcaaaatcgg cgtattttga ttacactcct gttaatccat acagcaacag
    18241 tactggggta acctggtact gttgtccgtt ttagcatcgg gcaggaaaag cctgtaacct
    18301 ggagagcctt tcttgagcca ggaatacact gaagacaaag aagtcacatt gacaaagtta
    18361 agtagcggcc gccgccttct ggaggcgttg ctgatcctta ttgtcctgtt tgccgtctgg
    18421 ttgatggctg ccttactaag ctttaaccct tcggacccca gctggtcgca aacggcctgg
    18481 catgaaccta tccataattt aggtgggatg cccggtgcgt ggttggcaga tacgctgttc
    18541 tttatttttg gcgtgatggc ttacaccatt cccgtcatta ttgtcggcgg ttgttggttt
    18601 gcctggcgtc atcagtccag cgacgaaaac attgattatt ttgccgtttc gctacgcatc
    18661 attggcgttt tggcgctcat ccttacctcc tgtggtctgg cggcaatcaa cgctgacgat
    18721 atctggtatt ttgcctccgg tggcgtcatt ggcagcttac taagcactac gctacaacca
    18781 ctgctacaca gtagcggggg aactattgcg ctgctctgcg tttgggcagc gggcctgacg
    18841 ttgttcaccg gttggtcatg ggtgaccatt gctgaaaaac tcggcggctg gattttaaac
    18901 attctcacct tcgccagtaa tcgtacccgt cgcgatgata cctgggtcga tgaagatgag
    18961 tatgaagacg acgaagagta tgaagatgaa aaccacggca aacagcatga atcacgccgt
    19021 gcccgtattc ttcgcggcgc gctagcgcgt cgtaaacggt tggcggaaaa attcattaat
    19081 ccgatggggc ggcaaacaga cgctgcgttg ttctccggta agcggatgga tgatgacgaa
    19141 gagattacct acactgcacg cggtgtggct gccgacccgg acgacgtcct attttcgggc
    19201 aatcgtgcaa cgcagccaga atatgacgaa tacgatccat tattaaacgg tgcgccaatt
    19261 accgaacctg tcgctgtagc atctgctgct accacggcga cacaaagctg ggctgcgccg
    19321 gttgaacctg tgactcagac gccgcctgtt gcctctgttg atgttccacc ttcgcaacct
    19381 acagtagcct ggcagcctgt accgggtcca caaacgggag agccggttat tgctcctgca
    19441 ccggaaggtt acccacagca gtcacaatat gcgcagcctg cagtgcaata taatgagccg
    19501 ctgcaacaac cagtacagcc gcagcagccg tattatgcac ctgcagctga acaacctgcg
    19561 caacagccgt attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc
    19621 gaagagcagc aatccacttt tgctccacag tctacatacc agactgagca aacttatcag
    19681 cagccagccg ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt
    19741 gtggagcctg aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactacttt
    19801 gaagaagttg aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg
    19861 attccagaac cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt
    19921 gcagcagtac ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa
    19981 aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca
    20041 aatagcggtg gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg
    20101 aaacgtatcc gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca
    20161 cagcgtgcgg cggaagaaaa agcccgtgaa gcccagcgca atcagtacga ttctggcgat
    20221 cagtacaacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc
    20281 cagacacagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaatgcagaa
    20341 gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa
    20401 cgttattccg gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt
    20461 tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgctgtttac gccaattgtt
    20521 gaacctgtac agcagccgca acaaccggtt gcaccgcagc agcagtatca gcagccacaa
    20581 cagccggttg cgccacagcc gcagtatcag cagccacaac agcaggttgc gccgcagcca
    20641 caatatcagc agccgcaaca accggttgcg ccacagcagc aatatcagca gccgcaacaa
    20701 ccagttgcgc cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag
    20761 tatcagcagc cacaacagcc agttgcgcca cagccgcagg atactctgct tcatccgctg
    20821 ttgatgcgta atggcgacag ccgtccgttg cataaaccga cgacgccgct gccttctctg
    20881 gatttgctga caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa
    20941 atggctcgcc tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgtcaat
    21001 tactctccgg ggccggttat cactcgcttt gaacttaacc tggcaccggg cgtaaaagcg
    21061 gcgcgcattt ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt
    21121 gtcgttgaag ttattcctgg caaaccctat gtaggtctgg agttaccgaa taaaaaacga
    21181 caaaccgttt atctgcgcga agtattggat aacgccaaat tccgcgataa tccatcgcca
    21241 ttaaccgtgg tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa
    21301 atgccgcact tgttagttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg
    21361 atgatcctga gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac
    21421 ccgaaaatgc tggagctttc ggtgtatgaa ggcattccgc acctgttaac ggaagtcgtt
    21481 actgatatga aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgt
    21541 tataaactga tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct
    21601 gaagccgatc gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg
    21661 gatgcccagc atccggtgct gaaaaaagaa ccatacattg tggtgttggt ggacgaattt
    21721 gccgacctga tgatgacagt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa
    21781 aaagcccgtg ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt
    21841 attactggtc tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag
    21901 attgactcac gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat
    21961 atgctctact ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc
    22021 gatcaggaag ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt
    22081 gatggcatca cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa
    22141 gaactggatc cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca
    22201 atttctggcg tacaacgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa
    22261 cagatggaag cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg
    22321 tccccaccgc cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc
    22381 ggcactctat caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg
    22441 gaatagagag tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa
    22501 aaaaattgcc atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga
    22561 tgccgcaagc gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac
    22621 acaaaaagtg actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt
    22681 gaaacgtcca aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc
    22741 tgacggtaaa acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct
    22801 gaaagatgcc accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg
    22861 gcagcagtac aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa
    22921 tggcaatctg aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag
    22981 cgcggtggag caggacgatc agcgcagcag ttatcaactg aaatcccagc aaaatggggc
    23041 tgtggatgca gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg
    23101 taagtagagg cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac
    23161 ctctggccgc gcgtatgcgg ccagaaaatt tagcacagta tatcggtcag caacatttgc
    23221 tggctgcggg gaagccgttg ccgcgcgcta tcgaagccgg gcatttacat tctatgatcc
    23281 tctgggggcc accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga
    23341 acgctgatgt ggaacgtatt tctgccgtca cctctggtgt gaaagagatt cgcgaggcga
    23401 tcgagcgcgc ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag
    23461 ttcaccgttt caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca
    23521 tcacttttat tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt
    23581 cccgtgcccg tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa
    23641 ctcaggcgat ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg
    23701 aaacacgacg tgccattgca gaactggtga atggcgacgc gcgccgggcg ttaaatacgc
    23761 tggaaatgat ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg
    23821 aattactgac cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct
    23881 tttacgatct gatttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc
    23941 tgtactggta tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct
    24001 gtctggcgat tgcgtctgaa gacgtcggta atgccgatcc acgggcgatg caggtggcaa
    24061 ttgcggcctg ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc
    24121 aggcgattgt ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag
    24181 ccgcgctggc cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg
    24241 cgccgacgaa attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg
    24301 aagcaaacgc ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct
    24361 attatttccc gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg
    24421 ctgaacagga tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg
    24481 ttgttactgt atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc
    24541 acaggataag catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa
    24601 aactggcacg ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc
    24661 gtaaagtatt gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat
    24721 ccattggcca ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca
    24781 aactgggcga agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc
    24841 gcgatatcgc gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg
    24901 aaaatgacaa cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc
    24961 gtgaccacgt gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc
    25021 tgactggttc ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt
    25081 cgcagtttat gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt
    25141 acctggttaa ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc
    25201 tgttccatac tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa
    25261 ctgcagaagt tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc
    25321 caattaagat gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg
    25381 acacccgtgg tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc
    25441 gcccggaaga ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc
    25501 agttgctggg cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg
    25561 cttgcaaaac ttacgacctg gaagtatgga tcccggcaca gaacacctac cgtgagatct
    25621 cttcctgctc caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca
    25681 agtcggacaa gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc
    25741 gtacgctggt tgcagtaatg gaaaactatc agcaagctga tggtcgtatt gaagtaccag
    25801 aagttctgcg tccgtatatg aacggactgg aatatattgg ctaataccca atttttctga
    25861 atctaaaaag cgcctacggg cgcttttttt gtctcccttt gataccgaac aataattact
    25921 cctcacttac acgtaatact actttcgagt gaaaatctac ctatctcttt gattttcaaa
    25981 ttattcgatg tatacaagcc tatatagcga tctgctatag aaataattac acaatacggt
    26041 ttgttactgg aatcaatcgt gagcaagctt gagtgagcca ttatgaaaac gaaaatccct
    26101 gatgcggtat tggctgctga ggtgagtcgc cgtggtttgg taaaaacgac agcgatcggc
    26161 ggcctggcaa tggccagcag cgcattaaca ttacctttta gtcggattgc gcacgctgtc
    26221 gatagcgcca ttccaacaaa atcaggcgaa aaggttatct ggagcgcctg tacagttaac
    26281 tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg gtgaaatcaa atatgtcgaa
    26341 acggacaata ccggcgatga caattacgac ggcctgcacc aggttcgcgc ctgcctgcgt
    26401 gggcgttcca tgcgtcgccg tgtctacaat ccggaccgcc tgaaatatcc gatgaaacga
    26461 gtcggggcgc gcggtgaagg caaattcgag cgcattagct gggaagaagc ctacgacatc
    26521 atcgcgacca atatgcagcg cctgatcaaa gagtacggca acgaatccat ctatctgaac
    26581 tatggcaccg gtacgctggg tggcaccatg acccgctcct ggccgccggg aaataccctg
    26641 gtcgcgcggc tgatgaactg ctgcggcggc tatctgaacc attacggcga ctactcctcc
    26701 gcgcaaattg cggaaggtct gaactatacc tacggcggct gggcagatgg caacagcccg
    26761 tcggatatcg aaaacagtaa gctggtagtg ctgtttggta ataaccctgg cgaaacgcga
    26821 atgagtggcg gtggggtgac ttactatctt gaacaggcac gccagaaatc taatgcccgc
    26881 atgatcatca tcgatccgcg ctataccgac accggtgccg ggcgcgaaga tgagtggatc
    26941 cctattcgtc cgggaacaga tgccgcactg gttaacggtc tggcgtacgt catgatcact
    27001 gaaaacctgg tggatcaggc attcctcgat aaatattgcg ttggctacga tgagaaaacc
    27061 ctgccagcca gtgcgccgaa aaatggccac tataaagctt atattctggg tgaagggcca
    27121 gatggcgtgg ctaaaacacc ggaatgggcc tcgcaaatca ccggtgttcc ggcagacaaa
    27181 atcatcaaac tggctcgtga aatcggcagt accaaaccgg cgtttatcag ccagggatgg
    27241 ggcccgcagc gtcacgctaa cggtgaaatc gcaacccgtg ctatctcgat gctggcgatt
    27301 ctgaccggta acgttggtat taatggaggg aacagcggcg cgcgtgaagg ttcatacagt
    27361 ttaccgtttg tccgtatgcc gaccttggaa aacccgatcc agaccagcat ttcgatgttt
    27421 atgtggaccg atgccattga acgtggcccg gaaatgacgg cgctacgtga tggtgtgcgc
    27481 gggaaagata agctggatgt gccgatcaaa atgatctgga actatgccgg taactgcctg
    27541 attaaccagc attctgaaat caaccgtacc catgaaatcc ttcaggatga taagaagtgc
    27601 gagctgattg tggttatcga ctgccacatg acctcatcgg cgaaatatgc tgacatcctg
    27661 ctgcctgact gcaccgcttc cgaacagatg gactttgcac tggatgcatc ctgcgggaat
    27721 atgtcttacg tgattttcaa cgatcaggtg attaaaccgc gctttgaatg taagaccatc
    27781 tatgaaatga ccagcgaact ggcaaaacgt cttggcgttg agcaacagtt tactgaaggc
    27841 cgtacccagg aagagtggat gcggcatctg tatgcccagt cgcgggaagc gattcctgaa
    27901 ctgccaacgt ttgaagagtt ccgcaagcag gggatcttta aaaagcgcga cccacaaggg
    27961 catcacgttg cttataaagc cttccgtgaa gatccgcagg caaatccact gaccacgcca
    28021 tcgggtaaaa ttgagattta ttcgcaggcg ctggctgaca ttgccgctac ctgggaattg
    28081 ccagaaggcg atgtgatcga tccactgccg atctacacgc cgggctttga aagttatcag
    28141 gatccgctga acaaacagta tccgctgcag cttacaggtt tccactataa atctcgcgtt
    28201 cactcaactt acggcaacgt tgatgtgctg aaagcggctt gccgtcagga aatgtggatc
    28261 aacccgcttg atgcccaaaa acgcggtatc cacaacggcg ataaagtcag gatctttaac
    28321 gatcgtggtg aggttcatat tgaggcgaaa gtgacgccac gaatgatgcc gggtgtggtc
    28381 gcactgggtg aaggtgcctg gtatgacccg gatgcaaaac gtgtcgataa gggtggttgt
    28441 attaacgtac tgaccactca acgtccgtct cctctcgcta aggggaatcc gtcacataca
    28501 aaccttgttc aggttgaaaa ggtgtaagga gtaaccgatg acaacccagt atggattttt
    28561 tattgattcc agccgttgca ccggttgcaa aacctgcgag ctggcttgta aagactacaa
    28621 agatttgatg ccagaagtca gcttccgccg catttatgaa tatgctggtg gcgactggca
    28681 ggaagataac ggtgtctggc accagaacgt gtttgcctac tatctgtcga tttcgtgcaa
    28741 ccactgcgaa gatccggctt gtactaaagt ctgcccgagc ggtgcgatgc ataaacgtga
    28801 agatggtttt gttgtggtcg atgaagatgt gtgcattggc tgccgctact gccatatggc
    28861 ttgcccgtat ggcgcaccgc aatataacga aacgaaaggc catatgacca aatgcgatgg
    28921 ttgttatgac cgtgttgcgg agggtaaaaa gccgatctgt gttgaatctt gtccgctgcg
    28981 ggcgctggat ttcggcccta tcgacgagct gcgtaaaaaa catggcgatc tggcggccgt
    29041 tgcgccgttg ccgcgagctc actttaccaa accgaatatt gtgatcaaac ccaatgccaa
    29101 tagccgcccg accggggata ccactggcta tctggcaaac ccgaaggagg tgtgagatgg
    29161 gaagtggatg gcatgaatgg ccgctgatga tcttcacggt cttcgggcaa tgtgtggcgg
    29221 gtggttttat cgttctggct ttggcgctgc ttaaaggcga cctgcgagca gaagcccagc
    29281 agcgtgttat cgcctgcatg tttggcttat gggtgctgat gggcattggc tttatcgcct
    29341 ctatgctcca tcttggttca ccgatgcgcg cctttaactc gctcaaccgg gtaggggctt
    29401 cagcactcag taacgaaatc gccagcggtt cgatcttttt tgctgtaggc ggcatcggct
    29461 ggttgctggc aatgctgaaa aagctgtcac ctgcattgcg tacgctatgg ctggtggtga
    29521 cgatggttct tggcgtcgtc tttgtctgga tgatggtgcg tgtgtataac agcattgata
    29581 ccgttccgac ctggtacagc atctggacgc cgatgggctt cttcctgacg atgttcatgg
    29641 gcggcccgtt actgggttac ctgttgctga gtctggctgg cgtcgatggc tgggcgatgc
    29701 gtctgctgcc agccatttct gtactggcac tggtggtgag tggcgtgatg tcagtgatgc
    29761 agggcacaga gctggcaact attcatagtt ctgtgcagca ggccgcagcg ctggtgccgg
    29821 actatggtgc gctgatgtcc tggcgaatcg tgcttttggc cgttgccctg tgcttgtgga
    29881 ttgcaccaca gctaaaaggt tatcagcctg cggttccgct actatccgtc tcattcattc
    29941 tgcttctggc aggggaatta atcggtcgcg gcgtattcta tggtttgcat atgaccgtgg
    30001 ggatggccgt cgcaagctaa tcagtaacaa ccggggtttc ggccccggtt ctcttttatt
    30061 tctgcttcgt taacgtgtca taactggtca tcaaattacg ataatcagga atgtggttag
    30121 agaacaacgt cgccagtcct tcaatatcat tacgccagtc acggtgcagc tcgcaagcca
    30181 cgccgaacca tgtcatcaat tgcgcacctg cttgcgacat gcgatcccat gccgaatgcc
    30241 gggtaatttc attaaaggtc cctgacgcgt cagtcacaac aaagacatca aacccttctt
    30301 caatggctga aagcgccggg aatgccacgc aaacttcggt taccacaccg gcaataatta
    30361 actgtttttt acctgtcgct ttgactgctt ttacaaaatc ttcgttatcc caggcgttaa
    30421 tattgccagg gcgagcaatg taaggtgcat cggggaattg tgctttcagc tctggaacta
    30481 atgggccgtt aggaccggtt tcaaaactgg tggtaagaat agttggtaag ttgaaatact
    30541 tagccaggtc acccagcgcc agcacgttat ttttaaactt atcgggttcg atatcccgta
    30601 caagggaaag taaaccagcc tggtgatcaa caagcaaaac ggcagcatca tttttatcaa
    30661 gacgaacata cggtttggtc atcctcttct cctttcgaga gtcagtggta cgtctgaaat
    30721 cggtcagacg cgatgcattg ctctgaaagc atagacggga aatatgagtt tgctgtgacc
    30781 atgaaatttt tcgactgaac gaagcgtatg aaatttgtgt tagatcaata aaaacaatca
    30841 gatgagatta atcatctctt cagcattagc gttatgaatg agtaatataa atcgataata
    30901 ccgccgctgc gatatgcgcg tattttcacc cattgacaat gttttttgcg gtggcatgat
    30961 gcgcatgaaa tttgaacttc ctcacggttt taattcatgt ccacgtatac ccggcctgtc
    31021 atgcttttgc tgtctggcct gcttttgttg actctggcga tcgcggtgtt aaatacactc
    31081 gtgccgcttt ggctcgccca ggaacacatg tccacatggc aggtaggcgt tgtcagctca
    31141 tcctatttta ccggcaacct tgtcggtaca ttgctgacag ggtatgtcat taaacgcatt
    31201 ggctttaacc gcagctatta tctggcctcc ttcatttttg ccgctggctg tgccggcctt
    31261 ggcctgatga ttggtttctg gagctggttg gcgtggcgtt ttgtcgcggg cgtcggctgt
    31321 gccatgattt gggtagtcgt tgagagcgcg ctgatgtgca gtgggacgtc acgtaaccgt
    31381 gggcgtttgc ttgctgcgta tatgatggtt tattacgtgg gaacattttt aggccagtta
    31441 ctggtcagca aagtctcaac cgagctgatg agcgtattgc cgtgggttac aggtttgacg
    31501 ttggcaggga tcttaccgct gttgtttacg cgtgtcctga atcagcaggc tgaaaaccat
    31561 gattcgacgt caattacgtc aatgctaaaa ctccgtcagg cgcggcttgg cgtgaatggc
    31621 tgcattatct caggaatcgt gctgggatct ctatatggcc tgatgccgct gtacctcaat
    31681 cacaaagggg tgagcaatgc cagcattggt ttctggatgg cggtactggt cagtgcgggt
    31741 atcctcggac aatggccgat tggacgtctg gcggataagt ttggtcgact gctggtgttg
    31801 cgtgttcagg tctttgtcgt cattctcggc agtatcgcga tgcttagcca ggcggcgatg
    31861 gccccagcgt tattcatcct cggtgccgct ggctttacgc tatatccggt ggcgatggcc
    31921 tgggcttgtg agaaagttga acatcatcaa ctggtggcga tgaaccaggc cttactgttg
    31981 agctataccg tgggaagtct gcttggcccg tcatttaccg ctatgctaat gcagaatttc
    32041 tccgataatt tattgtttat catgatcgcc agcgtatcgt ttatctattt gctgatgctg
    32101 cttcgcaacg ccggtcatac gccgaaaccc gttgctcacg tgtaaatgaa ttcaagcaaa
    32161 gtgtgaactt actgtttcac actctgcttt tttgtttctt ctatctgact tgcttttttc
    32221 caaattttat tcgtttaaaa ataaaatgtg cagcaggtta taattttgca tttcgctatt
    32281 tccgcacttc ttatttgccg cgcataatcc ctcgttttac agatgcccct ttaattttgg
    32341 cgaaggattt gtctatggct gggaatgttc aggaaaaaca gctgcgatgg tacaacattg
    32401 cgctgatgtc ttttatcact gtctggggtt ttggcaacgt tgttaataac tatgccaacc
    32461 aggggctggt ggttgttttt tcatgggtgt ttatctttgc gctctatttc acaccttatg
    32521 cactaattgt tggtcagtta ggctcgacct tcaaagatgg gaagggcggg gtcagtacct
    32581 ggattaaaca cacgatggga cccggattgg cttacctcgc cgcgtggacc tactgggtgg
    32641 tgcatattcc ctatctggca caaaaacccc aggcaatact gattgcgctc ggttgggcga
    32701 tgaaaggcga cggttcgcta atcaaagaat attcagtcgt agcgttacag gggttaacgc
    32761 tggtgctgtt tatcttcttt atgtgggttg cttcacgcgg tatgaaatcg ctgaaaatcg
    32821 tcggttctgt ggcagggatt gcgatgtttg ttatgtcgct cctgtatgtg gcgatggcgg
    32881 taaccgcgcc tgcgattact gaagtgcata ttgcgaccac aaacattacc tgggaaacgt
    32941 tcattcctca tatcgacttt acctacatta ccactatttc aatgctggtt ttcgcggttg
    33001 gcggagctga gaagatttct ccttacgtta atcaaacgcg caacccagga aaagaatttc
    33061 caaaagggat gttatgcctg gcggtgatgg ttgcggtttg tgccattctg ggctcactgg
    33121 cgatggggat gatgtttgac tcgcgtaata tcccggatga cttaatgact aacggtcagt
    33181 attacgcctt tcagaagctg ggcgagtatt acaacatggg taatacttta atggtgattt
    33241 acgccattgc gaataccctg ggacaagtag ctgcgctggt attctcgatt gatgccccgc
    33301 ttaaagtgct attaggcgat gctgacagca aatatattcc agccagttta tgtcgtacca
    33361 acgcttctgg tacgcccgtt aatggctatt ttctgaccct ggtgctggtg gcgatcctga
    33421 ttatgctgcc gactctcggc atcggtgata tgaacaatct ctataaatgg ctgttgaatc
    33481 ttaactcggt agtgatgccg ctgcgttatt tatgggtatt tgttgcattt attgcagtcg
    33541 ttcgcctggc gcagaaatat acaccagagt atgtctttat tcgtaataag cctctggcaa
    33601 tgaccgtcgg gatttggtgt tttgccttta ctgcctttgc ctgtttgacg gggatcttcc
    33661 cgaaaatgga agccttcact gcagagtgga ccttccagtt ggcgctgaat gttgcaacgc
    33721 cgtttgtgct ggtaggatta ggactgatat tcccgctgct ggcgcgtaaa gcgaatagta
    33781 aataattatt gtggtcggtg acgctgactc tggcagatct gacccgtttt tacataatcg
    33841 agaaatgcgc gcaagccgca ggacatatac tggcggtttg ggtaatacat ttggaagctg
    33901 tgctgtaagg gcgttagaaa tagagctctg aattatttat gcagcgcgga ataaaaaagc
    33961 ggcaactcaa taaagttgcc gctttacggg gaaattagaa cattacctta tgaccgtact
    34021 gctcaagaat acctttcacg cgttccatgg tttctttctt cggtggttta acaccatcga
    34081 gtttgtattc ttcacccatc gccacccatt tgtgtttgcc cagttcgtgg taggggagaa
    34141 gttcgatttt ctcaacgtta cccatatcac gggtaaattc accgaggcga tgcgctgaat
    34201 cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc acatttttat
    34261 tcgccagata tttggcgaac tccagcgtgc ggtggttgga aactccaacc agattttggt
    34321 ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact tccagcagtt
    34381 catcaatcac cggatcgtaa cgacgaacaa aaccgttggt gtccagacag gtatgaatgc
    34441 cttctttttt gcaggcgcgg aaccagtcac gaacaaactc agcttgcagg attgcttcac
    34501 cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag gtcaccactt
    34561 ccttcatcaa atcttcaacg gtaacttctt taccgccatg cgtgtcccag gtgtcgcggt
    34621 tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag cgaatacctg
    34681 ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact gacattgcgg
    34741 tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa ggctacgtcg
    34801 agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct cacgtgctaa
    34861 aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga tttcagtcaa
    34921 atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct gttctttagt
    34981 cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg gatatttttc
    35041 cggattttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt tcaggtgctg
    35101 accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagaccag ccaggttggt
    35161 cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg tgtaggagat
    35221 accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg ctacagcacc
    35281 tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc cagcacgacg
    35341 accgtctggg gtgttacccg ttttcttacc atacacaacg ttagaagtga tggtcagaac
    35401 agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct tcatgaaacg
    35461 ttctaccagg tcaacagcca ggtcatctac acgcggatcg ttgttaccaa actgcgggta
    35521 ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg gtttaacttt
    35581 cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga taccacacgc
    35641 catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt agctgtactt
    35701 gtcgtgcatg tagtgaatga tgttcagtgc agtgatgtac tgtttagcca gccagtccat
    35761 gaagtgatcc atgcgctcca tcacttcatc gtagttcagg agatcgcctt tgatcggttc
    35821 agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga ttgcgtacag
    35881 catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac caacgatcat
    35941 cgggcttacg cagcaagcga tagcgtagtc atcgttgttg aagtccggac gcatcaggtc
    36001 atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga atttcttgaa
    36061 gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag acggacccat
    36121 ggtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac gaccgtcgag
    36181 gcccatacca ccgatagatt cggttgccca gatcgggtcg ccagagaaca gttcatcgta
    36241 ctccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt caaccatttc
    36301 ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt acacatccag
    36361 gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa cagcagccag
    36421 gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac cagagatgtc
    36481 gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct gttcagcgat
    36541 ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat cagcctgcag
    36601 agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca gcgcaacgcg
    36661 acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg tcagaacacc
    36721 ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct ggttgtgagt
    36781 tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt tgtacgcttt
    36841 gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt tcagcggagc
    36901 ttcagtctgc agaccaacaa ctttttccaa cgctttgttg atgtagccag cgtcgtgaga
    36961 ggtgatggtg gaagcaacag cggtgtcgaa gtcaactggc gcgtgagtgc ggttttccag
    37021 tttaacgcct tccattactt tgtcccacag ggtggtggtc gcttcagtag cgccagccag
    37081 gaaggactcg tcaccctcgt acggagtgta gtttttctga atgaagtcac ggacgtttac
    37141 ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact tttcattaag
    37201 ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt acttcgacaa
    37261 ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc caaccaacaa
    37321 accaccgccg ataatattac cgatcgtaac cggaatcagg ttatcagtga tgaagttcat
    37381 cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga attccggaga
    37441 tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga tactgtgctc
    37501 aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccatg ataaacgctt tgtccatcag
    37561 gctgcggcca gaataactca tccatactgc cagacatacc atcaggtttg ccaggatacc
    37621 aaggcagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt gtaggacgtt
    37681 tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta aaagtacaaa
    37741 cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttag ccaactgacc
    37801 ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag taaagagatc
    37861 ggctccgcag acaacacaaa gaatcagtcc cagagagaag caaatgccgc caaccagttt
    37921 tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga agacgaatgc
    37981 gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg gatgtttcgt
    38041 tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa gtaaaagatc
    38101 aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac gagatactaa
    38161 caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta taggcccgta
    38221 actcgcatgg tttttatgca aatacggagt aaatatttga ttatccaaat aaaaataaat
    38281 tttaaaaatt aacaaatgag ttgaattttt tccgcatcct ccgctaaaac agttaattaa
    38341 aagggagcat caggcgaata aagtaacaat atcgatcgt
//
LOCUS       DAZFTN010000037        38376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFTN010000037 DAZFTN010000000
VERSION     DAZFTN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(112..303)
                     /gene="yfgG"
                     /locus_tag="ACHKV2_002058"
     CDS             complement(112..303)
                     /gene="yfgG"
                     /locus_tag="ACHKV2_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO6939939.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            321..473
                     /locus_tag="ACHKV2_002059"
     CDS             321..473
                     /locus_tag="ACHKV2_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6939940.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            655..2898
                     /gene="pdeF"
                     /locus_tag="ACHKV2_002060"
     CDS             655..2898
                     /gene="pdeF"
                     /locus_tag="ACHKV2_002060"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO6939941.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2937..4478)
                     /gene="ppx"
                     /locus_tag="ACHKV2_002061"
     CDS             complement(2937..4478)
                     /gene="ppx"
                     /locus_tag="ACHKV2_002061"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO6939942.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4483..6549)
                     /gene="ppk1"
                     /locus_tag="ACHKV2_002062"
     CDS             complement(4483..6549)
                     /gene="ppk1"
                     /locus_tag="ACHKV2_002062"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO6939943.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6720..7358)
                     /gene="purN"
                     /locus_tag="ACHKV2_002063"
     CDS             complement(6720..7358)
                     /gene="purN"
                     /locus_tag="ACHKV2_002063"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO6939944.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7358..8395)
                     /gene="purM"
                     /locus_tag="ACHKV2_002064"
     CDS             complement(7358..8395)
                     /gene="purM"
                     /locus_tag="ACHKV2_002064"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO6939945.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8720..9346
                     /gene="upp"
                     /locus_tag="ACHKV2_002065"
     CDS             8720..9346
                     /gene="upp"
                     /locus_tag="ACHKV2_002065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO6939946.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9432..10721
                     /gene="uraA"
                     /locus_tag="ACHKV2_002066"
     CDS             9432..10721
                     /gene="uraA"
                     /locus_tag="ACHKV2_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO6939947.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10816..11517
                     /gene="hda"
                     /locus_tag="ACHKV2_002067"
     CDS             10816..11517
                     /gene="hda"
                     /locus_tag="ACHKV2_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO6939948.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11655..12014)
                     /gene="arsC"
                     /locus_tag="ACHKV2_002068"
     CDS             complement(11655..12014)
                     /gene="arsC"
                     /locus_tag="ACHKV2_002068"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO6939949.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12035..13498)
                     /gene="bepA"
                     /locus_tag="ACHKV2_002069"
     CDS             complement(12035..13498)
                     /gene="bepA"
                     /locus_tag="ACHKV2_002069"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO6939950.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13711..14772
                     /gene="yfgO"
                     /locus_tag="ACHKV2_002070"
     CDS             13711..14772
                     /gene="yfgO"
                     /locus_tag="ACHKV2_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6939951.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14810..15658)
                     /gene="focB"
                     /locus_tag="ACHKV2_002071"
     CDS             complement(14810..15658)
                     /gene="focB"
                     /locus_tag="ACHKV2_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO6939952.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15680..17692)
                     /gene="hyfR"
                     /locus_tag="ACHKV2_002072"
     CDS             complement(15680..17692)
                     /gene="hyfR"
                     /locus_tag="ACHKV2_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO6939953.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17722..18135)
                     /gene="hyfJ"
                     /locus_tag="ACHKV2_002073"
     CDS             complement(17722..18135)
                     /gene="hyfJ"
                     /locus_tag="ACHKV2_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO6939954.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(18128..18886)
                     /gene="hyfI"
                     /locus_tag="ACHKV2_002074"
     CDS             complement(18128..18886)
                     /gene="hyfI"
                     /locus_tag="ACHKV2_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO6939955.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18883..19428)
                     /gene="hyfH"
                     /locus_tag="ACHKV2_002075"
     CDS             complement(18883..19428)
                     /gene="hyfH"
                     /locus_tag="ACHKV2_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO6939956.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(19438..21153)
                     /gene="hyfG"
                     /locus_tag="ACHKV2_002076"
     CDS             complement(19438..21153)
                     /gene="hyfG"
                     /locus_tag="ACHKV2_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO6939957.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21143..22723)
                     /gene="hyfF"
                     /locus_tag="ACHKV2_002077"
     CDS             complement(21143..22723)
                     /gene="hyfF"
                     /locus_tag="ACHKV2_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO6939958.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22728..23378)
                     /gene="hyfE"
                     /locus_tag="ACHKV2_002078"
     CDS             complement(22728..23378)
                     /gene="hyfE"
                     /locus_tag="ACHKV2_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO6939959.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23390..24829)
                     /gene="hyfD"
                     /locus_tag="ACHKV2_002079"
     CDS             complement(23390..24829)
                     /gene="hyfD"
                     /locus_tag="ACHKV2_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO6939960.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24846..25793)
                     /gene="hyfC"
                     /locus_tag="ACHKV2_002080"
     CDS             complement(24846..25793)
                     /gene="hyfC"
                     /locus_tag="ACHKV2_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO6939961.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25804..27822)
                     /gene="hyfB"
                     /locus_tag="ACHKV2_002081"
     CDS             complement(25804..27822)
                     /gene="hyfB"
                     /locus_tag="ACHKV2_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO6939962.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27822..28439)
                     /gene="hyfA"
                     /locus_tag="ACHKV2_002082"
     CDS             complement(27822..28439)
                     /gene="hyfA"
                     /locus_tag="ACHKV2_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO6939963.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28692..29162)
                     /gene="bcp"
                     /locus_tag="ACHKV2_002083"
     CDS             complement(28692..29162)
                     /gene="bcp"
                     /locus_tag="ACHKV2_002083"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO6939964.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29162..29734)
                     /gene="gcvR"
                     /locus_tag="ACHKV2_002084"
     CDS             complement(29162..29734)
                     /gene="gcvR"
                     /locus_tag="ACHKV2_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO6939965.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29880..30758
                     /gene="dapA"
                     /locus_tag="ACHKV2_002085"
     CDS             29880..30758
                     /gene="dapA"
                     /locus_tag="ACHKV2_002085"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO6939966.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30775..31809
                     /gene="bamC"
                     /locus_tag="ACHKV2_002086"
     CDS             30775..31809
                     /gene="bamC"
                     /locus_tag="ACHKV2_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO6939967.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32022..32735
                     /gene="purC"
                     /locus_tag="ACHKV2_002087"
     CDS             32022..32735
                     /gene="purC"
                     /locus_tag="ACHKV2_002087"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO6939968.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32903..33766
                     /gene="ypfJ"
                     /locus_tag="ACHKV2_002088"
     CDS             32903..33766
                     /gene="ypfJ"
                     /locus_tag="ACHKV2_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO6939969.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33781..35796
                     /gene="tmcA"
                     /locus_tag="ACHKV2_002089"
     CDS             33781..35796
                     /gene="tmcA"
                     /locus_tag="ACHKV2_002089"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO6939970.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35870..36568
                     /gene="ypfH"
                     /locus_tag="ACHKV2_002090"
     CDS             35870..36568
                     /gene="ypfH"
                     /locus_tag="ACHKV2_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6939971.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36649..36849)
                     /gene="ypfN"
                     /locus_tag="ACHKV2_002091"
     CDS             complement(36649..36849)
                     /gene="ypfN"
                     /locus_tag="ACHKV2_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO6939972.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36877..38004)
                     /gene="dapE"
                     /locus_tag="ACHKV2_002092"
     CDS             complement(36877..38004)
                     /gene="dapE"
                     /locus_tag="ACHKV2_002092"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO6939973.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38008..38364)
                     /gene="yffB"
                     /locus_tag="ACHKV2_002093"
     CDS             complement(38008..38364)
                     /gene="yffB"
                     /locus_tag="ACHKV2_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO6939974.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         9358 a         9909 c        10099 g         9010 t
ORIGIN      
        1 aagacgtata atttaagatg aaagaatatc tgccgctttg ataattccaa agagcaaaag
       61 catccgacag cgaagcgccg ttatgttctg tgaaagtgga gaaggtaacc gctaacgttg
      121 taccggtgat tcgacggaga gtgtggattg ctgagcttct tttttgctct gatggtgctg
      181 ccaggcaccg acggacgagt agataaaacg gccaaagaag aagataaagc tgatgagcag
      241 tacgatacgg gtcatgcgac tgttaaatcg gtgtcgtttt cgcatactgg ttgcctgact
      301 cacaaaaggt tccttgaagt atgtcccacg ccgtggacgg tacttacatt aaggcacaac
      361 aggacaaaat ggtcaattct tcgttatgta aaaaatcgtc agtggataca tattttaatg
      421 ttatggaagt taatttaatt atttacaatg atgatgtaat aatgatgaat taacatgata
      481 aacgtaataa ttcattaatc agggttattt tgtttgatat atatcaattg gaactttcat
      541 atgacactta gaatcaatgc tctctatagt gatgaataat catcattcga agtcaggtgg
      601 gatgcctgtc tgaatacacc tccttcagga tgtgggggat tctgctgagc atctatgaaa
      661 ctgaatgcaa cttatataaa aatacgtgat aaatggtggg ggcttccgct gttcctgcct
      721 tctttaatct tgcccatttt cgcccacatt aatactttcg cgcatatttc ttccggtgag
      781 gtttttctct tttatctgcc tctggcactg atgatcagca tgatgatgtt tttcagctgg
      841 gcggcattgc cagggatcgc cttagggatt tttgtccgca aatatgcaga gctgggtttt
      901 tacgaaacgc tctcattaac ggctaatttt attatcatta tcattctctg ttggggcggt
      961 tacagggtct ttactccccg gcgtaacaac gtttcacatg gtgatacccg tttaatttcc
     1021 cagcgtatat tctggcagat tgtgtttcct gcaacgctgt ttctgatact tttccagttt
     1081 gctgcatttg taggattact ggcgagcaga gaaaatctgg ttggcgtcat gccttttaat
     1141 ctcgggacct taatcaatta tcaggcctta ctggtgggca atctgattgg tgtcccgctg
     1201 tgctacttca tcattcgggt agtgcgaaat ccgttttatt tacgtagtta ttattcgcaa
     1261 ttaaaacagc aggttgatgt caaagtcacc aaaaaagagt tcgcactctg gctgctggca
     1321 ttaggtgctt tactgttgct gttatgcatg ccgttaaatg aaaaaagcac aatttttagc
     1381 accaattata ccttgtcatt attgctgccc ctgatgatgt ggggagcgat gcgctatggt
     1441 tataagctga tttcgctgct ctgggcggtc gtgttgatga tcagcatcca cagctatcaa
     1501 aattacattc ccatttatcc tggctatacc acgcagctga ccataacctc ctccagttat
     1561 ctggtattct cttttattgt caattatatg gctgtactgg caacccgtca gagaacggta
     1621 gtcagacgca ttcagcgcct tgcgtatgtg gacccggtgg ttcatctgcc aaatgttcgc
     1681 gccctgaatc gcgcgttgcg tgatgccccc tggtctgcgc tttgttattt acgcatccct
     1741 ggcatggaaa tgctggttaa aaactatggc atcatgctgc ggattcaata caagcaaaaa
     1801 ctttctcact ggctgtcacc cttgctggaa ccaggtgaag atgtttatca gctttcgggt
     1861 aacgatctcg cgctgcgact gaatacagaa tcgcaccagg agcgcattac cgcactggat
     1921 agccatctca agcaatttcg tttcttttgg gatggcatgc cgatgcaacc gcagattggc
     1981 gtcagttact gctatgtgcg ctcgccagtg aatcatatct acctgctgct gggagagcta
     2041 aatacggtcg ccgaactttc catcgtgacc aacgccccgg aaaatatgca gcgtcgcggg
     2101 gcaatgtatt tgcaacgcga attgaaagat aaagtcgcga tgatgaatcg actacagcag
     2161 gcgctggaac acaaccattt tttcctgatg gcccagccga ttaccggtat gcgtggtgat
     2221 gtttaccatg aaattcttct gcgcatgaaa ggtgagaatg atgaactaat cggccccgat
     2281 agcttcttgc cggtcgcgca cgaatttggt ttatcgtcga gtatcgacat gtgggtcatt
     2341 gagcatacgc tgcaatttat ggctgaaaac agagcgaaga tgcccgctca ccgttttgct
     2401 attaatctgt ctccaacctc ggtatgtcag gctcgttttc ctgttgaagt cagtcagttg
     2461 ctggataaat atcagattga agcgtggcaa cttatttttg aagtcaccga aagtaatgct
     2521 ctgaccaatg ttaagcaggc gcaaatcacc ttgcagcatc ttcaggaatt aggctgccag
     2581 attgcgattg atgatttcgg caccggctac gccagctatg cgcggcttaa aaatgtgaat
     2641 gccgatctgc ttaaaattga cggcagtttt atccgcaata ttgtgtcaaa tagtctggat
     2701 tatcagatag tggcatcgat ttgccacctg gcgcgaatga agaaaatgcg ggtagtggca
     2761 gagtacgttg aaaacgaaga gatccgcgag gcggtgctct ctttggggat cgattatatg
     2821 cagggttatc ttattggtaa gccgcaaccg ttaattgata cgctgaatga aatcgaaccc
     2881 attcgcgaaa gtgcctgaat aatgcgggcc gacatttctc gtcggcccgc aaagcattaa
     2941 gctgcgattt caggtgtact ttcttcttca attttcaacc gccagccagc cacgccttcc
     3001 cagtattctt gctccttttc cagatcaagc agtaccagcg cattctgact aaaccagtca
     3061 tgcgggaaac gcagtgtcca gtgactgtca tcagtaatca gcgtcaatgt tggcggtgtg
     3121 gtggttgcct gacgctgatt gttgagtaat acgccaaggc gcaatagctg aatcagtggc
     3181 aggaactgtt tcttcttaaa caaggtaaag cgcggcagat cgtcgagttt aatcgcttta
     3241 cggtgatagc gcaccagtgt cgccatcatc agctgctgtt cctgattaaa acccggcaag
     3301 tcgctgtttt gtagaatata agcggagtga cgatgcaaac cgctgtggtt gatattcaac
     3361 ccgacctcat gcagcatggc ggcccatcgc agtagcgcct ccagttgcgg atgcgccagc
     3421 ttcggttgct gttcccgcca ctgttcgtac atttgcatag tggtatccag cacccgtcgg
     3481 gcctgttcgc tgtcgatgtg atactggttg gcgaggctgc tggcggtgcg actacgcaca
     3541 tcctgatgac ggaaacgtcc ttccatttca tacagtacgc cttcgcgtaa cgccccgtca
     3601 gaaaggcgca gttcacggat ggctaaagca tcaaacacac cgcataaaat cgctagtcca
     3661 ggaacgaaga ctgttttccg ctcttcggaa agacctggta aactcagcga tgcgaaatta
     3721 cggtgccgta aaacttcttt taccagtttt tccagacgtt ccggggtaat tatcccgtct
     3781 ttctcgccca tttccattaa cacttcatgg gcggctttta tggtaccgga agcgcccatt
     3841 gccacgttcc agccctgaat acgaaattgc caggttaaag tttccagttt ttgtgctgcc
     3901 gccatgcgag cgcgctgaaa attctcttta ttgatgaccc cgccagggaa ataaagctgg
     3961 gcaaagctga cacaccccat ccggcggctt tcaacgagaa tcggttcgaa attttcacca
     4021 atcaccagtt ctgtagaacc gccgccaata tcaataacca gtttgcgacc tttttccggt
     4081 tgggtatgtt ccacgcccat aaaaatcaga cgggcttctt cattaccgga aataatttca
     4141 atcgggtagg gaatgacctt ttccgcacgt ttcagaaagt cagtggcgtt cagcgcctga
     4201 cgcagcgtat gggtaccaac tatacagacg ctggcaggag aaaacccttg tagccgttcg
     4261 gcaaacagcg acagacagtt caaaccgcgc gtcattgcct cttcactcaa catattatct
     4321 ggccccaggc cgtccgccag atgcacccgc tgtttcaggc ggccaataat ctgcatggca
     4381 ccatctacca cacgggctat gaccatgtga aaactgtttg aaccaagatc gaccgcagca
     4441 aactcctgcg gacgagggga tttatcgtgt attggcatag ggttattcag gttgttcgag
     4501 tgatttgatg tagtcataaa tcgccaactg cgcccgtact ttgcggcgat tgccgcgggg
     4561 aacgtagcga ttactgagtt ctttatcgat ataacgtgct ttgaccgtat cgctgaacaa
     4621 tatgtcgatg atgtccagca cccgctgctt caggcgcgga tcgagcagcg gcgtcgccac
     4681 ttcaatacga taatcaatat tgcgcgtcat ccagtcggcg gaagaaaggt agaccttttt
     4741 atcgccgcca ttttcaaaaa tataaacccg gtcatgttca aggtaacggt caacaatact
     4801 gatggcacga atgttgtcgc taatgccttc cagattgggg atcagcgaac acattccgcg
     4861 aaccagcaga ttaaccggta cgccggagct ggaggccgca tacagacgat caaccaagcc
     4921 tttatcgaca aggttattta gcttcagggt gataccactg ggcagccctt gctgcgcgtt
     4981 ggcgatctcg cggtccacca tttcatacaa caggcggcgg gagttttgcg gcgacaccat
     5041 taaataatca aatgtcaccg gacggtatgg gttttcaata aagttaaata cccgccgtac
     5101 ttcgttggtg atgcgcgcat cggcggtcag caacgaatag tcagtataaa gacgcgcggt
     5161 tttttcgtta aagttcccgg tcccgatgtg tgcataacgc accacttcac cgttttcttt
     5221 acgtgaaatc agaaacagtt tggcgtgaat tttcagcccc ggcgcagaga agataacgtg
     5281 cacgcctgct tcggtcaggc gcttcgccca gtgaatgttg gcttcttcgt caaaacgtgc
     5341 ctgtagctca accaccacgg tgactttctt accgttgtgt gcggcgtgga tcatcgagtc
     5401 gatgatgcgt gaatcttttg ccacacggta aatgttgatt ttaatcgcca gcacgctcgg
     5461 atcgaacgag gcctgacgca gcagttccag cacatgctca aaggtgtgat aaggataata
     5521 gagcaacaca tcgcgttcgc gaatggcatc aaaaccattg cggaactggg ctttatcaaa
     5581 ccaaatatgg cgtaaacgcg gcagtggttt gttcaccaga ttggctttgc cgacattggg
     5641 gaaattaata aagtctttaa aattatgata acgaccgccg gggacgatgg agtcgtagcg
     5701 ggaaatagtc agtttttcgc gtaacacttc aaccagcgca ttgggcatat cgcgctgata
     5761 aacaaaacgc accggctcag cagttaaacg ctgcttgaga ctggaagaca tcaactccat
     5821 caggctggct tccatctcat gcactaaatc gtattcggca tcgcgggtca tcttcattga
     5881 ataggcattc agcgcgtcat aatcaaagaa gcctttgaaa atatcatcaa ggcagtaacg
     5941 cagaatgtta tccagaagaa tcattggctt gcgtcgacgc ggcgcttctg gcggtaaatt
     6001 cacaaagcgc ggcactttat ctgatgggat ctccagaagc gcgtaacgga tggtatcgcc
     6061 acggataatt tccaccgcca gataggtgta atcatctttc aggaactgca ctaagtcagt
     6121 gtcaggattg attaaaatcg gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg
     6181 cagccagttt tgttgattga cggagagctg gcgttcatta atcaggaaga tctggttgcg
     6241 cgccatctcc agcaatagct cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac
     6301 ccgggactga attttgccca gtaaatggcg ggaatgagag ttggagcctt gttcttcgct
     6361 aataatgatg cgtcgcttca gttcagcgaa gcggacttta tagaactcat caaggttatt
     6421 ggaatagatc cccaggaaac gcatcctttc aatcagcggg ttagatttgt ccgccgcttc
     6481 ctgaagcacg cgttcattga acgataacca actgagctct ttttcgatgt atagcttttc
     6541 ctgacccatt accactttta ctccgtttta ttcacgggac actgcctgga tattatggcg
     6601 agcaatattg gcagatgtcc aactatgcca gaaaaggggg aaaaaaactg ctggagcttg
     6661 cgatttacag gtgaattgaa aaacgccagc ggcagagctg gcgctttaac tacggggcat
     6721 tactcgtcgg cagcgtagcc ctgcggcggc agacgttgac catccagcca cgcggcgttt
     6781 tcgtgcattt tcagacgacc atcggcaaac cagctaatca ccagtggata aatggcgtgt
     6841 tcctgggttt gcacgcgggc ggtgacgtca tcctccgtat caccagcaaa taccgggact
     6901 ttcgcctgta aaataaccgg gccaccgtcc agttcatcgg tgacgaaatg caccgatgta
     6961 ccgtgctctt catcgccatt ttccagcgcc tgacgatggg gatgtaatcc gggatatttc
     7021 ggcagcagag aagggtgaat gttcagcaaa cgcccggcat agtgggagac aaatgccggg
     7081 ctgagaatgc gcataaaacc agccagcacg accacatcgg gtgcgtacat gtcgatttca
     7141 tgaatcaact cccggtcata ggcttcacga ctgtcaaacg cgctggcgat gagcgtatgc
     7201 gttgcaatac ccgcctggcg ggcgcgttca aggccgaacg cgtcggcctt attgctgaaa
     7261 actgcccgta cggtgccttt aattttgttg gttttacagg cgtcaataat tgcctgcaaa
     7321 ttacttccgt tgccggaaat aagcaccaca atattcatta ttcgataacc acgcgttgtt
     7381 cggaatcaga ggctttgatg ataccgattt tccacgcgtt ttcaccgttg gcattgagca
     7441 gggcgagggc tttgtccact tccggagcag gcagggcaat aatcatcccg acgccgcagt
     7501 tgaaggtgcg atacatttca tggcgctcaa cgttaccagc cgtttgcagc cagttgaaca
     7561 cttccggcca ctgccaggag gattcatcaa tcactgcctg ggtattatct ggcaatacgc
     7621 gcggaatgtt ttcccaaaag ccgccgccgg tcaggtgcgc aatggcatta acatcgacct
     7681 tttcaatcaa ctccagcact gacttcacgt aaatgcgggt cggtgccagc agatgatcgg
     7741 ctaatggctt accatcaagt tcggtggttt gcggatcaca accgctgact tcaagaattt
     7801 tgcgcaccag cgaatagccg ttcgagtgcg gaccgctgga accgagtgca atcagcacat
     7861 cgccgtcgct gactttagag ccgtcgatga tttctgattt ttctaccacg cccacgcaga
     7921 aacccgcgac atcgtaatcc tcaccgtgat acatccccgg catttctgcc gtttcgccac
     7981 ccaccagtga acagcccgat tgcagacaac cttccgcaat gccgctgatc accgctgaag
     8041 cggtatcaac atccagtttt ccggttgcgt aatagtcgag gaaaaacagc ggttctgcac
     8101 cttgcaccac caggtcatta acgcacatgg cgaccagatc aataccaatg gtgtcgtgac
     8161 gttttaagtc cattgccaga cgcagcttgg tacctacgcc gtcagtgccg gaaaccagca
     8221 cgggttcacg atatttttgc ggcaatgcac acagcgcacc gaagccgccc agaccgccca
     8281 tcacttccgg acgacgcgtt ttcttcacta cgcctttgat tcttccaacc agagcattac
     8341 ccgcgtcaat atcaacaccg gcatctttgt agctaagaga ggttttatcg gtcactgctt
     8401 gggtccccac gcgttacttg cggtagaaaa ataaaattcg gcgcaattct aacagggaaa
     8461 gcaaacgttt gcgagactgc tttacacaac ctttttgcac gtcttttccc caggcgcgcg
     8521 gcgaaagaag acttgtgcca gggcaaaggt tagttttcgg atggaataat cttctttcat
     8581 aaccatttga atataaaata actttatctc aaaccgttat cattttgact aaagtcaacg
     8641 aaaataatat tgccgccttg aagaaaggag gtataatccg tcgatttttt ttgtggctgc
     8701 ccctcaaagg agaaagagta tgaagatcgt ggaagtcaaa cacccactcg tcaaacacaa
     8761 gctgggactg atgcgtgagc aagatatcag caccaagcgc tttcgcgaac tcgcttccga
     8821 agtgggtagc ctgctgactt acgaagcgac cgccgacctc gaaacggaaa aagtaactat
     8881 cgaaggctgg aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa ttaccgttgt
     8941 gccaattctg cgtgcgggtc tggggatgat ggacggtgtg ctggaaaacg ttccgagcgc
     9001 gcgcatcagc gttgtcggta tgtaccgtaa tgaagaaacg ctggagccgg taccgtactt
     9061 ccagaaactg gtttctaaca tcgatgagcg tatggcgctg atcgttgacc caatgctggc
     9121 aaccggtggt tccgttattg cgaccatcga cctgctgaaa aaagcgggct gcagcagcat
     9181 caaagttctg gtgctggtag ctgcgccaga aggtatcgct gcgctggaaa aagcgcaccc
     9241 ggacgtcgaa ctgtataccg catcgattga tcagggactg aacgagcacg gatacattat
     9301 tccgggcctc ggcgatgccg gtgacaaaat ctttggtacg aaataaagaa taaaaataat
     9361 taaagccgac tttaagagtc ggcttttttt tgagtaaagc gcctataaca cataatacag
     9421 aggataatac tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca cttttacaga
     9481 caatcccgct tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc ctggtgcccg
     9541 tcttatttca tattaacccg gcgactgtac tgttatttaa cggtattgga acgctgctgt
     9601 atctcttcat ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt gcctttattt
     9661 caccggtatt gttactgttg ccgttagggt atgaagtcgc gctgggcggc tttattatgt
     9721 gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa agcggggacc ggctggctgg
     9781 acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt ctggagctgg
     9841 cgggcgtagc tgccggtatg gcgggtttac tcccggctga agggcaaacg ccagactcca
     9901 aaaccatcat catctctatt accaccctgg cggtcacggt tttaggttcc gtgctgttcc
     9961 gtggtttcct ggcaattatc ccgattttaa ttggcgtgct ggtggggtac gcgctctctt
    10021 tcgcaatggg aattgtcgat accacgccga ttattaatgc tcactggttt gcgctgccaa
    10081 ccctctatac gccgcgcttc gagtggtttg ccattctgac tattctgcca gcggcgttag
    10141 tggttattgc cgaacacgta gggcacctgg tagtaacggc taatatcgtc aaaaaagatc
    10201 tgctgcgcga tccaggtctg caccgttcga tgtttgctaa cggcttgtcg accgtgatct
    10261 ccggcttctt tggctccacg ccaaacacca cctacgggga aaacattggc gtgatggcga
    10321 tcacccgtgt ttacagtacc tgggttatcg gcggggcggc gattttcgct atcctgcttt
    10381 cctgcgtcgg taaactggct gccgctatcc agatgatccc attgccggtg atgggcggcg
    10441 tttcgctgct gctttatggt gtcatcggtg cttccggtat tcgtgttttg atcgaatcga
    10501 aagtggatta caacaaagca cagaacctga tcctgacttc cgtgattttg atcatcggcg
    10561 tcagtggcgc gaaggtaaac attggcgcgg cggagctgaa aggtatggcg ctggcgacca
    10621 tcgtcggtat cggcttaagt ttgattttca aactgattag tgtgctgcgc cctgaagaag
    10681 tggtgctgga cgcagaagac gcggatataa cagacaaata atgtaccgac cgggcagtgt
    10741 tgctgcccgg ttcaaacatc atgggattct gtggtaaact tctcgcgatt ttgtgaaatc
    10801 ctggttgagg tatctctgaa cacaccggca cagctctctt tgccacttta tcttcctgac
    10861 gacgaaacct ttgcaagttt ctggccgggg gataactcct ctttactggc cgcgctgcaa
    10921 aacgtgctgc gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg
    10981 cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc
    11041 tatgtcccgc tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat
    11101 ttgtcgctgg tctgtatcga caacattgag tgtattgcag gcgatgagtt gtgggagatg
    11161 gcgattttcg atctctacaa tcgaattctg gaatcgggca aaacacggtt gttgatcacc
    11221 ggcgatcgtc caccgcggca gttgaatctg ggattaccgg atctcgcgtc gcgactcgac
    11281 tgggggcaga tctacaaatt gcagccactt tctgatgaag ataagttgca ggcgctacag
    11341 ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag
    11401 cggctcgaca gagaaatgcg cacgctattt atgacgttgg atcagttgga tcgtgcgtcg
    11461 attaccgcgc aacgtaagct gaccattccg tttgtgaaag aaattctgaa gttgtagata
    11521 aggtgtttat tgtcggatgc ggcgcgaacg ccttatccga actacgcgaa gttaatccat
    11581 agaattcgta ggccggataa gacgtggcaa gcgtcgcatc cggcaatgtg cttcaactag
    11641 cgatggagca catcttaacc cacgatttcc agtacctgct ccggtggacg accaatccgc
    11701 gctttgccat tcgcgaccac aatcgggcgc tccatcagct tcggattatc caccattgcc
    11761 tgaatcagcg cctcttcgct aagcgaactg tcagccaggt tcagttcttt ataaagatcc
    11821 tctttctggc gcatcaattc acgggcgcta ttcatcccca gcattttcag caaatcgcgc
    11881 agcgttgccg catcggcggg tgtctcaagg tagagcacca cttctggctc cacgccgttt
    11941 tctttcagca ggttcagcgt ttcccggctc tttgaacagc gtgggttatg gtagattttt
    12001 acctgtttgg tcattgttct tcctttaatg cgaattacat cttggtataa ggcttaaagc
    12061 gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta acgcgcttgt tgcaggctgc
    12121 ctaatttcac ctgcgaactg gcgctactca acagcgaaat ggcctgatcg agtcgtccgg
    12181 cgagcgcata accttctgct cgcgcagcca gctcctgatc gcggttattt agcgcggctt
    12241 ccgcctgtgc cagcaaatcc cagccgttgc tgtcatcttt attattaaag gtgtagcgat
    12301 taagaatatt ggccgcttct tgtggttgac cgccttgtag ataagcgttc gccaggttga
    12361 gctgcaacac aggattggtg cgcaaatcgc gggcattttt cagacgattg atcgcctcat
    12421 tggctttgtt ttgcccaaga tcgatatcag tagccagatc gagataccat gcgttgccag
    12481 gttctgccgc cagtaacggt tgcagcgttt ttcgcgcctc gtcgtattta ttggcttcca
    12541 tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg acgaacgttt cctttcgccc
    12601 attcatccag caaatcactg gtgagctggt tacgtccgga attatacatc cccagtgtgc
    12661 gcgctttcgc cagatagaaa tcttccgacg actgcaccac catcgggcgc atctgattag
    12721 cacggttgcg ggcatctgcc agacgacttt ccggcaacgg gtgagtcagt aaaatttccg
    12781 gcgggcgcga ggagtaacgc gcctgatcga gtaatttttc gaggaaggtt ggcatcgcct
    12841 gcggatcgaa tcccgagcgt tgcagcacct gaataccaat gcggtccgct tcctgttcat
    12901 tttgctgggt gaaactgatc atcccctgac gcgttcccgc cagtgtaccg gtcagcgccg
    12961 ccatccccgc ctgcggactg gccatcgcca gtaaaataga acctaacgcg ccgacccagg
    13021 tcagcggcgc gttgcgctgc tgatcttcca tcgctcgcgc caggtgacgt tgggtgacgt
    13081 gggagatttc gtgcgccata actgaagcca gttgactttc gttatcggaa taacggaaca
    13141 gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa ggcgttaatt tcgtcgttgt
    13201 tgatcagaaa gaaatgaaac ggtgtcttaa ccgaattggc atgcgaaacc agacgcatcc
    13261 ccagcgaatt aatatattgc gttaacagcg ggtcattaat taacggcgcg ctgccgcgta
    13321 gctggcggac ataatagtcg cccatctgca tttcctgacc aatggaaagc gtgcttcctg
    13381 cggaggttcc catatccggc aaggtgtctg cgctgtcggc aaatgccggg gctacctgac
    13441 caatggtcat agcagcaatg agggttgcaa ccaggttttt tttcaactgc ctgaacataa
    13501 cctctatcct gtatttctga agatagtcat ttgaccgata ctgtgaaata tcgttcccac
    13561 cgcgccgcct ttgagtgtaa cggcgaagat tagcccactc aaatgacttt tcagtttcct
    13621 gaatccgtca taaaaagcga agtctttttt gtgacatttc gatacaattg cggatcgcaa
    13681 tcccgctctt gaggttcagg gaaggtcttt atgctcgaaa tgttgatgca atggtatcgc
    13741 cgccgtttta gcgacccgga agcgattgcc ttgctggtta ttttagttgc cggatttggc
    13801 attatctttt tctttagtgg cctgcttgct ccgttgctgg tggctattgt gctggcctat
    13861 ttgctggaat ggccaaccgt gcgcctgcaa tctattggct gctcccgccg ctgggcgacg
    13921 tcgattgtat tggtggtttt cgtcggtata ttgctactga tggcgttcgt ggtactgcct
    13981 atcgcctggc agcagggcat ctacttaatc cgcgatatgc cggggatgct caataagctt
    14041 tctgactttg ccgccacgtt gccgcgccgc tatccggcgt taatggatgc gggcattatt
    14101 gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc ggtggtgaaa
    14161 atttccctcg cctcgctggt cggtttgctg accattgccg tctatctggt gctggtacca
    14221 ttgatggtct tcttcctgct gaaagacaaa gagcagatgc tgaatgccgt tcgccgggtg
    14281 ctgccgcgta accgtggact ggcaggacag gtgtggaagg agatgaatca acaaatcacc
    14341 aactatatcc gcggcaaagt gctggagatg atcgtagtag ggatcgccac ctggctggga
    14401 ttcttactct tcgggctgaa ctattcgctg ctgctggcgg tgctggtcgg cttctcggta
    14461 cttattccgt acattggcgc atttgtggtg accattccgg tggttggcgt ggcgctattc
    14521 cagtttggtg caggcactga attctggagc tgtttcgcgg tgtatctgat cattcaggcg
    14581 cttgacggca acctgttagt accggtgttg ttctccgaag cggttaacct gcatccgctg
    14641 gtgattattt tatcggtggt gatcttcggt ggtttgtggg gattctgggg cgtattcttc
    14701 gccattccat tggcgacgct gatcaaagcc gtgatccacg cctggcccga tgggcaaata
    14761 gcgcaagaat aatcttcagc ccgacgaact gtttcgccgg gctgattttt caggattcct
    14821 gacgtaaata aatagcccga taacaaatac tcaccagcac tgcaccgccg ataatattac
    14881 ccagcatcac cgggagcaga ttggcggtaa taaaatggct gaccgtcagt gcaggaaaat
    14941 tgtctgcgct actgtgcgcc agttgccaga aggaggtagg ggcgaaatgg cgaatggcaa
    15001 tggcgaacgg aatcacaaac aaattggcga tacagtgctc aaagccactg gcgacaaaca
    15061 gggtgatggg caaaatcatg gcgacgattt tgtcgcataa cgaacgcccg caataactca
    15121 tccacagcgc caggcaaacc atcagattgc acataatgcc gaggctgaca gattcagtaa
    15181 atgtatgatg cattttgccc tcggcgcagt gtaaaaccgc gactccccac atcgcgtttt
    15241 cactcatcac cagcccggaa aaccagatta acaaactgaa acaggcaata cctgccagat
    15301 taccgcaggc caccagaagt gcgttaatca gccaagttcg ccaactaata acgccccgac
    15361 tttttgccat caccgtcatt accgacgagg tgaacaggct ggtgccgcaa accgccagca
    15421 aaataaagcc gagtgtaaag caaaggccgc ccaccagatg ggttaatgcc tgtgaagacg
    15481 gggcatcggc gataacggaa aggtaaaaag taaatccgat cgccataaac acgccagcgg
    15541 acatcgccat taaaaagacc gacactttac tgcgggcaat tttatgggcg gcaatccgtt
    15601 cagcgattgc cgcttttctg gcgctcaact gcaagtcgaa agagagtttg tttctcatgc
    15661 atactcctgc aaaagcagat tacaacacct cgcgaaccga gatccccaga cgctgcattc
    15721 gtgacagcag cgtggtgcgc ttcatcccta atcgtgtcgc cgcgccacgg ggcccggcaa
    15781 caatgccatt ggtttctcgc aatacctgaa caatgcgctg acgttcttct tcgtcattct
    15841 ctggcgtcgt cgggtgcagc aactgcgcca ttgaactgcg aagcgctgaa tcttcattta
    15901 gcgtcggcag taaacggctt tgtcggacat taagatgtaa attcagactg ttaccgcgag
    15961 tcagcagtac cgctcgctca atcacgtttt ccagctcgcg cacgttgccc ggccaatccc
    16021 acgacatcaa ctggcgtagt gcctcggtcg ggatggcgtc aattgcgcga ttcatatggc
    16081 gcgccatttt ttgcgtgaaa tgttttgcta aaagagggat atcttccgga cggtctcgca
    16141 gcggcggcaa ttccagtggg aagacattca ggcgataaaa gagatcgctg cgaaactggc
    16201 gatcttcaac catttgccac aaatcacggt tggtggcggc aatgactctg acattcaccg
    16261 ggatcgttct actcccgccg agacgctcaa tttcccgctc ctgcaatacg cgcagcaatt
    16321 taggctgaag ttctaacggc agatcgccaa tttcatcgag aaacaacgta ccgccatcgg
    16381 caatttcaaa acgaccacga tgggtattaa tcgcaccagt aaaggcccct ttgtcatgac
    16441 cgaataactc actttccaga agactggcgg ggatggcagc gcagttgatt ttgaccagcg
    16501 gcttgtcgcg tcgcgggcta agttgatgga tcgctctggc gatcacctct ttgccggttc
    16561 cggtttcacc acaaatcaac accgtactgt cgctcttcgc cacaatatct acctgctgga
    16621 gcaggtcttc cattgcctgg ctttgataga taatgtcacc gacgcccaga ttcgaaagga
    16681 gctgctcgct aagctggtgg ttttcttgct gcaaactttc ctgcaaatcg gtcatgctac
    16741 gccaggcatc ggcattgcca acggcaatag cgatgcgatc ggctatgtgt tgtagtagct
    16801 ggcagttttc ctcactaaag agagtggtag aggtatgcgc cagcaacaat gcacccggtg
    16861 tatggttgcc aaaggtaagc ggtatcagga gcgaagattc gcagccgtta gatgcaagta
    16921 agtgtaataa cgggtcgcgt tgccagagaa acagatcgtc tgcacggtgc gtcaaggtcg
    16981 gttgtcgggt ttgtagcgtt tgtgtcagca atacactgtc gccaggcata ttgcgtggca
    17041 gacacgcaca atgtgaggca gaaagatcgc tgcaccacag gctgaacttc tcgttctttc
    17101 gatgatcgcc cagtaccata ctgactgaag ccagaccgaa aaaatgatgg atctcacgag
    17161 cgacgtcagc gatcagatcg tcgagatcaa gatgtgacag cactgcatta gtgatatcca
    17221 ccagtacccg atgatgatcg cgatctttct ccagcaaacg cagcgccacc tgttgttctt
    17281 catcattgag ccatgattgc agaaccgggg tgataatgcg tgccagccag ttaaacagtg
    17341 tgcaatcctg gggagtaaaa cgactgccgt gggtgcgaat aaagcgtagc tccgccattc
    17401 cactactgcc gagaaggacg gcgtgacgcg aataatcgac catctcgccc tgatactctg
    17461 ttgttgccgt cacatcgctg ccatcatcat ggtagcggta atagaagttg cttttgagaa
    17521 catgcagttc aataagttgt accgggctga acggcggcgg cagcgggatg aaggcgcgta
    17581 ataaagacgc cttgccgtgt ttctgcgcta accgctctgc gagcattccg tttaccgctt
    17641 caattgttat tccttgcggc ggggcaaaca tcgcctcgtc tgacatagcc atcaacagtc
    17701 cttattaatg agaaatcctt ctcacgcaat ttttctggcc atcagataga tagccggttc
    17761 cagcacaatt tcatccaaca gatgaatgaa ttgaatgcta cgcgcttgcc acacagacgt
    17821 cgcgtcatct gccagtggcg cgaatgcccg ggtcaacagg gcggtgtggc tggcgtcaat
    17881 aacaatctca ccaaaagtca tcacccccag catcttacgt cgggcttgct cctcttcgac
    17941 cagtgcaagc caatcttcgt attccgtcag tgggcagcgg aaggtgacat tcagacaatc
    18001 aatcacgcca acgtgatgac cgatagccag cgagtaatac attacctggg agctgtgttc
    18061 cggcatctcg tcgctactgg cgacaaactt ttttcgcagc gtccagaaaa caatttcccc
    18121 gcactcttca gtcatgtaac ccccggagta cgcgaaagag ttgctgaacg atactattga
    18181 gacgtggatc gtcggcatcg cgcaaccagg tgttaacccg atttcctgta ggatcgttgc
    18241 ttaaatggcg caggagccga tcgcaaattt cttgcccctg acgatagccc gccagccgcc
    18301 gcgcttctcg ctcaatggcg atacgctgtg atggcgggat ctgcggccac aacggttgca
    18361 tagtcacccc ggtgggatcg cgataatcca ccgcgtgaat cttctgttgc agcaaaccga
    18421 gcgccacggc gaaaccgtga atggtggcgg ccggtgttgg cgggcagccg gggatccaaa
    18481 catcaatggg gacaatggtg tcgctgccgc cccagacgct gtagagatcg tggaaaatac
    18541 cgccgccgac accgcacgcg ccgtaggaaa cacagatttt atgatcgggg gcagactcat
    18601 acgcccgaag tgcaggcata cgcatcgccc gggtgactgc gccagtaaat aacaaaatat
    18661 cggcgtgacg cggtgatgaa acaaccttaa tgccaaaacg ttctgcgtcg aatacgggtg
    18721 tgatggcggc aaaaatttcg atttcacagg cgttgcagcc gccgcaatcg acgcgataaa
    18781 cgtaggctga gcgacggata tcctgtagca aatgccgctt catcttttgc gtttgctcgt
    18841 ccagcgtgat gggctggctg acatgttgtg taagcactgg actcatagct gctccttagc
    18901 caccagcggt acatctgtat cgtcgttgat cagcgtcgcg cgttgtttgc actccgggca
    18961 gacgctcgct tgcgcccgca acatttcgcg gttttgtggc gcattttgtt gctgtgccaa
    19021 caattcagca gccagtgcga cggttttttg cggggcaaac gggcgttcgc aacggctgca
    19081 acgttgtaga tggaacgtcg cgcgggtata gagatcggct ttattggtga cggtcagttc
    19141 aaagttattg gtaagctgga tggctctggt cgggcacact tcttcacaac gtccgcagta
    19201 aatacaacgc cccagataga gctgccaggt gcgcgaattt tgctggtcgt cggtctggat
    19261 agtcagcgca tttgtcggac aagcacaggc gcaggcacca caggcaatac attggctggg
    19321 catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga agggatattt
    19381 caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct tctgtcctta
    19441 cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat acggcacggt
    19501 ttttgactgg cgcttgcgca catcaaccag cgtcacacgg tcggtacagg agtagcaggg
    19561 atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca gcatgtaacg
    19621 caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca atttttggtt
    19681 atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa agccgagcgc
    19741 gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg gcatgttgtc
    19801 gagagcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga ccatcacgcg
    19861 ggagaaaaca tcgccgccgg tgaaggtaaa cagtgttttt ggaatattgc cgtagtcggc
    19921 gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca gcggccctac
    19981 agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc
    20041 catattcggc gtggcaagca gcatttctac cagctccgac acgtcggcgc gcatctcgcg
    20101 caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat
    20161 cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg tcatcgactt
    20221 ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat ggcaggagag
    20281 gccaaggttg agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg agcgaatggt
    20341 atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg tataggccac
    20401 actgtgggca aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta
    20461 gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca gacggtaatc
    20521 agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt catcggaggt
    20581 gatatgcagc gggccgacag ggatcacccg cgcaccgctg ttgccctcat tgataaacgg
    20641 atacgtttcg gaatcagtcg tcggttcagg gcgcagtcga taatccatcg catctttgcg
    20701 cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag
    20761 gccaaccgga atcagcccgt acatatcacg aatttctcgc tcgccccaga ccgcggcagg
    20821 gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagcg ccttcaccac
    20881 aatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt aatgaccgtt
    20941 aagtgtccgc tcgtcgttgc caaacaggac cggaagccag ccatcatgtt gataataaag
    21001 atactgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaggcgt
    21061 ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc
    21121 ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc
    21181 ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga
    21241 ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata tgcgttccca
    21301 tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccgagatcac
    21361 cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc
    21421 ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg tgattacgtg
    21481 ccagtccggc ggtaacggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg
    21541 ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc
    21601 cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga
    21661 acagcagcgt ttttgccaga ctgtggttta aggtgtgcag cagcgcggca aaaattccca
    21721 gcggcccgcc aatgcctagc gccaccgcga ccagtcccat gttctccacg ctggagtacg
    21781 ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca
    21841 acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggcc tggcaaataa
    21901 tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagtaggg
    21961 cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata
    22021 gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg
    22081 gatcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg
    22141 gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac
    22201 caacagtaca aataatgatg tacttccatg ctgcttccag cgatgaacgc tgaccgtaaa
    22261 tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca
    22321 ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt
    22381 aatcgcacag cgtaacgggt gaaagctcgc cgtgctcaac ttcatgacgc atgtagccaa
    22441 tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac aaaccgccca
    22501 gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca gcggcagttt
    22561 ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt aatactgtca
    22621 ccgtgcgagt cgcagagtgc cctcgtttcc ggcaggcaaa acagagcagc gaaaaaagca
    22681 gcggagtgag caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc
    22741 gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac
    22801 accatcacaa tgacggcgaa gatggcgtcg gtagcgatac caatttccac cagttccggt
    22861 gcacgccagg ctagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca
    22921 aaaatttgcc gcaggatatt gcgctggcta acaatgcaca gcaggccaag cagaaaatga
    22981 cctaacgcca ccgccagcgc tggtttcagc ccggtagcca tcggtagctt cacgggctga
    23041 acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg
    23101 aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac
    23161 ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg
    23221 gcgaacaggc acgagagggt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa
    23281 aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc
    23341 agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc
    23401 aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc agcaccaggc
    23461 gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg ccggggacga
    23521 cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc atcagcagaa
    23581 tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccggca gcaaacagcg
    23641 ggaatttact gaagaagccg ttgaacggcg gtacgccggt aatcgccagc gctgccacgc
    23701 agaaacccac gcctggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc
    23761 gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg
    23821 cgtggttgac gatgtaggcg atgctgccct ccagcgccag ccgcgagccg aagatggaga
    23881 gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct
    23941 tcatatcctg ctgtggcaaa tacatcagaa atccataaag aatggtgacc agggccatga
    24001 ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa
    24061 aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg
    24121 gcgcttccat tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg
    24181 cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg
    24241 cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca
    24301 acgaaccgat atgggtgata agcaacgctt ttagtgctga acgctgcgct ttatcgctct
    24361 gataataact gatcaacacc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac
    24421 cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc agcaaaaatg
    24481 cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg
    24541 tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtacaca
    24601 cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga
    24661 cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac agcgtggtca
    24721 gcgcggcgaa caaaaccccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca
    24781 gtgcgccgat aaaaggcagc aataacgtcg tcagagcaag attttccata ttccgtcagt
    24841 gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc
    24901 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    24961 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    25021 gcgagagaaa gttcttgcgc gcacccaaag ggcagaaaca gggccacaaa cagtgctgcc
    25081 atcacgacct gtttcagccc cagcccccat ttcgccagcg ccagcccggc accggaatat
    25141 tctgtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    25201 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    25261 gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    25321 agcgccaata ccagcagtga gagaatgagc attggttcga ccagaatgcc gagcgtcaac
    25381 tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag
    25441 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    25501 ggggaaacgg taataaatag tggtagggcc atcgccagca ccagcatgct gctgattaat
    25561 acccacggca tcaggcggaa catcagacct gaagatatag gcgcaacttc ctggcgttta
    25621 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    25681 cgcgcgcgta tctgccggga aatacccgta aaaagcgggg ttagcatcag cagaatcacg
    25741 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    25801 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    25861 gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct
    25921 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    25981 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    26041 tagcgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc
    26101 attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    26161 cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa
    26221 cagtaatgag ggggtgacct gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    26281 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    26341 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    26401 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    26461 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    26521 caccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    26581 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa gggctgatat
    26641 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    26701 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag
    26761 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    26821 ggtgagcagc gggtcgtgta gtgacagacc gaccatcgcc acaccgactg ccagcaaaat
    26881 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    26941 cgcatacagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    27001 ccacagcggc aaacccgttt gcgccagcag atccatcgcc actttcagga tgccgaaaat
    27061 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    27121 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    27181 ggccagcagg aacaccgccg acgccagccc cggagaaagt gaaagcgtgc ggaaactggc
    27241 aaaatctagg ctgccgcttt cgcgccccat cagcaagaag gcgatcataa tcagcaccga
    27301 tccggcgtgg gcgataaaaa agtagagcat gccagcgttg atcgacgttt tatcctgcct
    27361 ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga tgaaccaaaa
    27421 agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaatattca taaagaagcc
    27481 catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat agagcgaaca
    27541 aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat ccattcgcag
    27601 ggttaaatcg gcaaagtcaa aaggggtggc aaaacgggca actaaagtta cgccttcatg
    27661 taattgcgta attccgctga tgatgccaat cacgccgccc accagcgatg tgatgccgga
    27721 aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac tggcaaacag
    27781 atagaggatc agcgaccagg ttaataattg cagggcatcc attagcgttg ctcctgagtg
    27841 agggaaaggg gatcctccct gccattggca aaccagcttg cggcaaggcg ctgtttttct
    27901 ttcatctgac gttgcaggct atcgtcggtg atcagccgta acgcctgatt cgggcaagcg
    27961 cgaacgcagg caggaccttc tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc
    28021 tggacacccg gttcccagcg cagcaaagca tgttgtgttg gcgcgttttc ttcgccgtct
    28081 ttgagtgagc cttcagcctg aaaaacatat tgcgcagggg cattcaccgg acggcttcct
    28141 gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc aatacagagg
    28201 ctttcgttga gttggatcgc atcatccctc tgggagatgg cattgaccgg gcagacctgc
    28261 acgcaagggg cttcctcaca gtgatgacac acgacagggg cagtgagtgt tgacgtcttc
    28321 gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca
    28381 gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa gcggttcatg
    28441 tcggctcctt gaaaatgata tgaaacattg catttcataa tgcataaagg atgtcagtat
    28501 gattaattta atatattcaa taagttatga attaactgaa ttctgaacgg tacaaaaatg
    28561 atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg gaaagtgaga
    28621 atgtgatctt gatcacgata agtgaggtgt gctgaccgca agcgcagcca gcacggaatg
    28681 gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg gtgattgctg
    28741 gttttgaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag gaagctgatg
    28801 cgatgaatac catcgtaggt tttgcccatg aaggactttt ccccccagac gccgaattgc
    28861 tcgcacacct ggtggtcctc atcagacagg agcgtaaagt taagcagctc tttttccgca
    28921 aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac gcccgctttt
    28981 ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc gggggtcatg
    29041 gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa attaacttgt
    29101 tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt cagtggattc
    29161 attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta atactgcctt
    29221 gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct gcgtccgcag
    29281 atgcggggct gtgggcagta atctgaatat gcaactgcgc agcccgttca ttttcagcag
    29341 gttgtgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga
    29401 agcgttcaat taaatgcggg gagtctgcca catcgacctg aacccagaca gatgctggca
    29461 ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc agttcggcac
    29521 ctttcaacgg taacgttgat tcaatcagag taatggcatt ccatgaaccg gaaagcagca
    29581 tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata ttacagccgc
    29641 aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca cccaacgcag
    29701 tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta aggggtgagt
    29761 tgttcttaag gaaagcataa aaaaaacatg catacaacaa tcagaacggt tctgtctgct
    29821 tgcttttaat gccataccaa acgtaccatt gagacacttg tttgcacaga ggatggccca
    29881 tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc
    29941 gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg gcgatcgttt
    30001 ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct gatgtggtga
    30061 tgatgacgct ggagctggct gacgggcgca ttccggtgat tgccgggacc ggtgctaacg
    30121 ctactgcgga agccattagc ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc
    30181 tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag catttcaaag
    30241 ccatcgctga gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct
    30301 gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca
    30361 aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt tcagatgatt
    30421 ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaatta ggcggtcatg
    30481 gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg
    30541 cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg ccattacaca
    30601 acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc
    30661 ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga
    30721 cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga tttgatggct
    30781 tactctgttc ataagtcgcg cctggcaacg gttgcgggtg tttcgcttgt tttattactc
    30841 gctgcctgta gttctgactc acgctataag cgtcaggtca gtggtgatga agcctacctg
    30901 gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc
    30961 ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc gctggacatt
    31021 cgtccaccag cccagccgct ggcactggtt tctggcgcgc gtacccagtt cacgggcgat
    31081 accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca ggtggttagc
    31141 gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca gacactgacc
    31201 accgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa
    31261 atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgct gaacctggaa
    31321 caggcgggca aaccggttgc agacgcggct tccatgcagc gttacagcac ggagatgatg
    31381 aacgttattt ccgctggtct ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat
    31441 cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt accaatgctg
    31501 gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg
    31561 ggcatgaaag tgaccgacag cacccgttcg cagggcaaca tggccgtaac ttataagccg
    31621 ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc atctggtgac
    31681 tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat cgatccgaaa
    31741 ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt
    31801 agcaagtaaa aatacagggc tggaatcatc cggccctttt ttctgatatg atacgcaaac
    31861 gtgtgcgtcg gcaggaaaac gcgattttag cggtaattcg cacgaaattt gtttgtcgga
    31921 cgtagttcgg ataaggcgtt cacgccgcat ccgacaaaac atccggcaca ccagacagca
    31981 aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag caagctgagt
    32041 tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg ttggtgctcg
    32101 aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag
    32161 gtatggtgaa caacaagttc aactacttca ttatgagcaa actggctgaa gcgggtatcc
    32221 cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg
    32281 tgccggttga gtgtgtcgtg cgtaaccgtg ctgctggctc tctggtgaaa cgtcttggaa
    32341 tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa aacgacgcca
    32401 tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggta agcaaagaga
    32461 acctggcacg catgaaagag ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg
    32521 atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac aaaggtgaag
    32581 tggtactggg cgatgagttc tccccggacg gtagccgcct gtgggacaaa gaaacgctgg
    32641 agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgag gcctatgaag
    32701 ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca tcttgccgtg
    32761 ctgctggcac ggcaagacaa ccattctcgt aagatgtgca ttgaacgctc attcctccgc
    32821 catttcatcc cgttttcctg tggtaatctt cctctgaacc acctacgatc atttctataa
    32881 tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga
    32941 agacaggcgc aatagctccg gcggtccgtc aatgggcggt cccggtttcc gtctgccgag
    33001 cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact atggcgttga
    33061 tttaaccggg ttgatgaccg ggcagccggt ttctcaacag caatcaacgc ggtcaattag
    33121 cccaaatgaa gacgaagccg caaaattcac ctcggtgatt ctggcaacca cggaagacac
    33181 ctggggacaa cagttcgaga agatgggtaa gacctatcag caaccgaagc tggtcatgta
    33241 ccgtggaatg acgcgtaccg gatgcggggc gggccagtcc ataatggggc cgttctattg
    33301 cccggcggat ggtacggttt atatcgatct ctccttctat gatgacatga aagacaaact
    33361 tggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg gtcatcatgt
    33421 gcagaaactg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc
    33481 ggaagtgaac cgcttatctg tgcgtatgga actccaggcc gactgttttg ccggtgtctg
    33541 ggggcatagc atgcagcagc aaggcgttct ggaaaccggc gatctggaag aggcgctgaa
    33601 cgcggcgcag gccatcggcg atgaccgttt acaacagcaa agtcaggggc gagtagtacc
    33661 agacagtttc actcatggca cttctcagca acgctacagc tggtttaaac gtggtttcga
    33721 cagcggcgat ccggcacaat gcaatacttt tggtaaaagc atttaacttc cggggcaggg
    33781 atggctgaac tgactgcgct tcacacatta acagcgcaaa tgaaacgtga agggatccgc
    33841 cgcttgctgg tgttgagcgg ggaagagggt tggtgttttg atcatgcgct taagttacgt
    33901 gatgccttac ctggcgactg gctgtggatt tcgccgcagc cagatgctga aaaccactgt
    33961 tctccctcgg cactacaaac tttacttggg cgcgagttcc ggcatgcggt attcgacgcc
    34021 cgccacggct ttgatgccgc tgcctttgcc gcacttagcg gaacgttgaa agcgggaagc
    34081 tggctggttt tgttactccc tgtatgggaa gagtgggaaa accaacctga tgccgactcg
    34141 ctgcgctgga gtgattgccc tgaccctatt gcgacgccgc attttgtcca gcatttcaaa
    34201 cgcgtactta cggcggataa cgacgctatc ttctggcggc aaaaccagcc gttctcgttg
    34261 gcgcatttta ctccccgtac tgactggcac cccgcgaccg gcgcaccaca accagaacaa
    34321 cagcaaatct tacagcagct actgaccatg ccgtcgggcg tggcagcggt aactgctgcg
    34381 cgtgggcgcg gtaaatcggc gctggcaggg caactcattt ctcgtattgc gggtagtgcg
    34441 attatcactg cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc gggcgagaag
    34501 tttcgcttta ttgcgcctga tgccttgtta gccagcgatg agcaagccga ctggctggtg
    34561 gtcgatgaag ccgcagccat acctgcgccg ttgttgcatc aactggtatc gcgttttcct
    34621 cgaacgttgt taaccactac ggtgcagggc tacgaaggta ccggacgtgg ttttttgctg
    34681 aaattttgcg ctcgctttcc gcatttacac cgttttgaac tgcaacagcc gatccgctgg
    34741 gcacagggat gcccgctgga aaaaatggtt agtgaggcac tggtttttaa cgatgaaaac
    34801 ttcacccata caccacaagg caatatcgtc atttccgcat ttgaacaaac gttatggcga
    34861 agcgagccag aaacgccgtt aaaggtttat cagttattgt ctggtgcgca ctaccggact
    34921 tcgccgctgg atttacgccg catgatggat gcaccagggc aacatttttt acaggcggct
    34981 ggcgaaaacg agattgccgg agcgctgtgg ctggtggatg aggggggatt atctcaagaa
    35041 ctcagtcagg cggtatgggc aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg
    35101 ctggcggcgc acggcagcaa tccactggcg gcgacattgc gtggacggcg ggtcagccgg
    35161 atagcagttc atccggcgcg tcagcgcgaa ggcgttgggc aacagctcat tgccagcgct
    35221 ttgcaatata ggcctggcct cgactatctt tcggtgagtt ttggttacac cggggagtta
    35281 tggcgtttct ggcaacgctg cggttttgtg ctggtgcgaa tgggtaatca tcgtgaagcc
    35341 agcagcggtt gctatacggc gatggcgctg ttaccgatga gtgatgcggg taaacagctg
    35401 gctgaacgtg agcattaccg tttacgtcgc gatgcgcaag ctctcgcgca gtggaatggc
    35461 gaaacactcc ctgttgatcc actaaacaat gccgtccttt ctgacgacga ctggcttgaa
    35521 ctggccggtt ttgctttcgc tcatcgtccg ctattaacat cgttaggttg cttattgcgt
    35581 ctgctacaaa ccagtgaact ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgtc
    35641 agcgacgcgc agttatgtac cacacttaaa ctttcaggcc gcaagatgtt actggtccgt
    35701 cagcgggaag aggccgcaca ggcgctgttc gcacttaatg atgttcgcac tgagcgtctg
    35761 cgcgatcgca taacgcaatg gcaatttttt cactgactcc ttcaattttc tcccatggtc
    35821 attgtgagtc agcggcgtaa aattaatgac atcagttaag gagttcgcca tgaaacatga
    35881 ccattttgtt gttcaaagcc cggataaacc tgcgcaacag ttgttactgc ttttccacgg
    35941 tgtcggggat aacccggtgg caatggggga aataggtagc tggtttgcac cgttgttccc
    36001 ggatgcgctg gtggtgagtg tcggtggcgc ggagccgagc ggtaatccgg cggggcgtca
    36061 gtggttttcg gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg atgccatcat
    36121 gccgacgttt attgaaacgg tgcgctactg gcagaaacag agcggagtag gggctaacgc
    36181 cacggcgcta atcggtttct ctcaaggcgc gattatggcg ctggagagca ttaaagccga
    36241 accgggcctt gcttcacgcg tcattgcttt taacggacgc tatgcgagcc tgccggaaac
    36301 cgcgtctaca gccacgacaa ttcacttgat tcatggcggt gaagatccgg ttatcgatct
    36361 ggcgcatgca gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga
    36421 tatcgtggag gatttaggcc atgcgatcga caatcgcagt atgcaatttg ccctggatca
    36481 cttgcgttat accattccga aacattattt cgacgaagcg ctaagcggcg gtaagcctgg
    36541 cgatgatgat gtgattgaaa tgatgtaata atagccgggt aggcctgata agaccgtatc
    36601 aggcatcatg gccggatgcg gcgttaacgc cttatccggc cacttagctt acttcttcgg
    36661 ttgatccttt ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg
    36721 gggaagctcc ggcttgttgg cgagaaattt cttatggtcg acgcgcttga gatctttaat
    36781 cacgttcagc aggacgccta ccaaaaagac aatcaccaga atccaccaat atttagccag
    36841 ccagtccatg cttatttcct cttgcagaac cactcatcac gcgacgagct gttccatgat
    36901 acgttgatac atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt
    36961 atgaatagtg gcattgaccg gcccgagttc caccacctgc gcccccatgc gggcaataaa
    37021 gcgcccgtcg gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc
    37081 aaccgcgtta acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga
    37141 aagccaccaa tccaccgtat agcgcagttg atgtttttca agcagggcaa gcacctgcgc
    37201 tttgatcatc tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc
    37261 acccggaata acgttgttac tgcccgttcc cgcctgaata ttggcaatct gcatactggt
    37321 cgccgggaag aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg
    37381 tgctgcgcga tgtaccggat tgtcagccag atgtgggtag gcaacatgcc cctgaacgcc
    37441 atgaatggtt aggttgcagg ttaacgatcc acgacgacca tttttcacca catcacctac
    37501 cacttcgata ctcgacggtt cgccgaccag gcagtaatcg agacgctcat tacgtgccat
    37561 taacgcttcg acggctttta ccgtaccatt gtgggcactg gcttcttcat cagaggtgat
    37621 cagaaatgcc agtcgccctg tatggttggg atgttgtgcg acaaaacgtt ctgcagctac
    37681 caccatcgcc gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccgtc
    37741 acgaatggtt ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac
    37801 gtcggtatgc ccggcaaagg ccaatgtttc accttgccca cgccatgccc aaaaattctg
    37861 cgtatcggca aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa
    37921 caaagcctgg catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt
    37981 cagctcaata accgggcacg acatagacta cacctcatgg aaaaatttct gataactgga
    38041 atcactgaaa cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat
    38101 tgcaggcatt tcagtcatta atgccgccgc agaggccgca tcggtgatta tattgcgggt
    38161 ggtttcgtcc agtttacgcc aggttgtacc acgggtgttg agtaacgctt cccagcctaa
    38221 ttcgttgata aaaccgttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa
    38281 acgatagtcg atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt
    38341 tttgatgccg taaagtgtaa ccataatgaa tccttt
//
LOCUS       DAZFTN010000038        38297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFTN010000038 DAZFTN010000000
VERSION     DAZFTN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            11..949
                     /gene="ytfI"
                     /locus_tag="ACHKV2_002094"
     CDS             11..949
                     /gene="ytfI"
                     /locus_tag="ACHKV2_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO6939975.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(1012..1566)
                     /gene="ytfJ"
                     /locus_tag="ACHKV2_002095"
     CDS             complement(1012..1566)
                     /gene="ytfJ"
                     /locus_tag="ACHKV2_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6939976.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1891..2097
                     /gene="ytfK"
                     /locus_tag="ACHKV2_002096"
     CDS             1891..2097
                     /gene="ytfK"
                     /locus_tag="ACHKV2_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO6939977.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2210..3553)
                     /gene="paeA"
                     /locus_tag="ACHKV2_002097"
     CDS             complement(2210..3553)
                     /gene="paeA"
                     /locus_tag="ACHKV2_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO6939978.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3876..4514)
                     /gene="msrA"
                     /locus_tag="ACHKV2_002098"
     CDS             complement(3876..4514)
                     /gene="msrA"
                     /locus_tag="ACHKV2_002098"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO6939979.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4720..6453
                     /gene="tamA"
                     /locus_tag="ACHKV2_002099"
     CDS             4720..6453
                     /gene="tamA"
                     /locus_tag="ACHKV2_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO6939980.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6450..10229
                     /gene="tamB"
                     /locus_tag="ACHKV2_002100"
     CDS             6450..10229
                     /gene="tamB"
                     /locus_tag="ACHKV2_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO6939981.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAELFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10232..10573
                     /gene="ytfP"
                     /locus_tag="ACHKV2_002101"
     CDS             10232..10573
                     /gene="ytfP"
                     /locus_tag="ACHKV2_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO6939982.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10785..11036
                     /gene="chpS"
                     /locus_tag="ACHKV2_002102"
     CDS             10785..11036
                     /gene="chpS"
                     /locus_tag="ACHKV2_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO6939983.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            11030..11380
                     /gene="chpB"
                     /locus_tag="ACHKV2_002103"
     CDS             11030..11380
                     /gene="chpB"
                     /locus_tag="ACHKV2_002103"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO6939984.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11460..11990)
                     /gene="ppa"
                     /locus_tag="ACHKV2_002104"
     CDS             complement(11460..11990)
                     /gene="ppa"
                     /locus_tag="ACHKV2_002104"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO6939985.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12300..13256
                     /gene="ytfQ"
                     /locus_tag="ACHKV2_002105"
     CDS             12300..13256
                     /gene="ytfQ"
                     /locus_tag="ACHKV2_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO6939986.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13396..14898
                     /gene="ytfR"
                     /locus_tag="ACHKV2_002106"
     CDS             13396..14898
                     /gene="ytfR"
                     /locus_tag="ACHKV2_002106"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO6939987.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQARRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14912..15934
                     /gene="ytfT"
                     /locus_tag="ACHKV2_002107"
     CDS             14912..15934
                     /gene="ytfT"
                     /locus_tag="ACHKV2_002107"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO6939988.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15921..16916
                     /gene="yjfF"
                     /locus_tag="ACHKV2_002108"
     CDS             15921..16916
                     /gene="yjfF"
                     /locus_tag="ACHKV2_002108"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO6939989.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16949..17947)
                     /gene="fbp"
                     /locus_tag="ACHKV2_002109"
     CDS             complement(16949..17947)
                     /gene="fbp"
                     /locus_tag="ACHKV2_002109"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO6939990.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            18123..19496
                     /gene="mpl"
                     /locus_tag="ACHKV2_002110"
     CDS             18123..19496
                     /gene="mpl"
                     /locus_tag="ACHKV2_002110"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO6939991.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19652..20203)
                     /gene="yjgA"
                     /locus_tag="ACHKV2_002111"
     CDS             complement(19652..20203)
                     /gene="yjgA"
                     /locus_tag="ACHKV2_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO6939992.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20297..21649
                     /gene="pmbA"
                     /locus_tag="ACHKV2_002112"
     CDS             20297..21649
                     /gene="pmbA"
                     /locus_tag="ACHKV2_002112"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO6939993.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21833..22219
                     /gene="cybC"
                     /locus_tag="ACHKV2_002113"
     CDS             21833..22219
                     /gene="cybC"
                     /locus_tag="ACHKV2_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6939994.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22264..22728)
                     /gene="nrdG"
                     /locus_tag="ACHKV2_002114"
     CDS             complement(22264..22728)
                     /gene="nrdG"
                     /locus_tag="ACHKV2_002114"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO6939995.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22827..24965)
                     /gene="nrdD"
                     /locus_tag="ACHKV2_002115"
     CDS             complement(22827..24965)
                     /gene="nrdD"
                     /locus_tag="ACHKV2_002115"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO6939996.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25359..27014)
                     /gene="treC"
                     /locus_tag="ACHKV2_002116"
     CDS             complement(25359..27014)
                     /gene="treC"
                     /locus_tag="ACHKV2_002116"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO6939997.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27064..28485)
                     /gene="treB"
                     /locus_tag="ACHKV2_002117"
     CDS             complement(27064..28485)
                     /gene="treB"
                     /locus_tag="ACHKV2_002117"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO6939998.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28604..29551)
                     /gene="treR"
                     /locus_tag="ACHKV2_002118"
     CDS             complement(28604..29551)
                     /gene="treR"
                     /locus_tag="ACHKV2_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO6939999.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29736..29789
                     /gene="mgtL"
                     /locus_tag="ACHKV2_002119"
     CDS             29736..29789
                     /gene="mgtL"
                     /locus_tag="ACHKV2_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO6940000.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29930..32626
                     /gene="mgtA"
                     /locus_tag="ACHKV2_002120"
     CDS             29930..32626
                     /gene="mgtA"
                     /locus_tag="ACHKV2_002120"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO6940001.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32832..33218)
                     /gene="ridA"
                     /locus_tag="ACHKV2_002121"
     CDS             complement(32832..33218)
                     /gene="ridA"
                     /locus_tag="ACHKV2_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO6940002.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33291..33752)
                     /gene="pyrI"
                     /locus_tag="ACHKV2_002122"
     CDS             complement(33291..33752)
                     /gene="pyrI"
                     /locus_tag="ACHKV2_002122"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO6940003.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33765..34700)
                     /gene="pyrB"
                     /locus_tag="ACHKV2_002123"
     CDS             complement(33765..34700)
                     /gene="pyrB"
                     /locus_tag="ACHKV2_002123"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO6940004.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34704..34838)
                     /gene="pyrL"
                     /locus_tag="ACHKV2_002124"
     CDS             complement(34704..34838)
                     /gene="pyrL"
                     /locus_tag="ACHKV2_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO6940005.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35119..35514)
                     /gene="yjgH"
                     /locus_tag="ACHKV2_002125"
     CDS             complement(35119..35514)
                     /gene="yjgH"
                     /locus_tag="ACHKV2_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6940006.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35645..36358)
                     /gene="bdcA"
                     /locus_tag="ACHKV2_002126"
     CDS             complement(35645..36358)
                     /gene="bdcA"
                     /locus_tag="ACHKV2_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6940007.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36429..37022
                     /gene="bdcR"
                     /locus_tag="ACHKV2_002127"
     CDS             36429..37022
                     /gene="bdcR"
                     /locus_tag="ACHKV2_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6940008.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37167..37619
                     /gene="tabA"
                     /locus_tag="ACHKV2_002128"
     CDS             37167..37619
                     /gene="tabA"
                     /locus_tag="ACHKV2_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO6940009.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37742..>38297
                     /gene="yjgL"
                     /locus_tag="ACHKV2_002129"
     CDS             37742..>38297
                     /gene="yjgL"
                     /locus_tag="ACHKV2_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO6940010.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDE
                     SSLHLITNKVLACYQREANKEIARL"
BASE COUNT         9314 a         9442 c        10236 g         9305 t
ORIGIN      
        1 aggttgtcat atgttaccca ggatcagaca caataatttt attggtgcgg tggagttatt
       61 tgtaaagtct tcgtatacaa aaacacattc aaacaatttt tttaacaaca tccatcatgc
      121 atttaagaaa aaagattgga tttcgaatta tgatagcctg ttaactttga gggagttctt
      181 tcgttgcgca actcagattg ataaaagtag ttatcaggtg ctttcatcaa aaaatgaaac
      241 tgttaacgct atggataagt ttcttatcag tttcagcctt aaagataacg gtgccgaata
      301 tacaatgaca ttgcgaggca gtggatttga atacgaagaa atccccataa caataaatga
      361 gtataactca ttcatggatt tcaagaaccg tgaatttccg ttagaacaaa acagacgatt
      421 gtatgcctgg gatattctgc agaaaaaaca gtctgacata ccgaaaagga ttaaaggtta
      481 tattcaccag gcgattggtg acgtgtcttt aggttatgct ttattggatg atatagtaag
      541 caaattaaaa cggggtaaat ttgaactaca agggccagga ggtggaatta aacagtgcga
      601 tggatggtat atttacgaaa aaataattga tgataatttt gccatagtca tagaatcttt
      661 gggctttgcg cttaaaatct atggagggga tgaacgtttt cgtaatggtt cctctgttgt
      721 cttggaggat gaagattact ctcttattta taattttctg gttaatgcgg gttgccaaca
      781 agtcgaatta gccgaacaag tggatgcgat agtaagtgcg aatctggccg cagatagtga
      841 tattaccaaa gaaaagattt gcgagaaata taaatcgacc attgaggcgt ttaaaaaaga
      901 gcaactagcg ttaccagtat tagttcggcg taagaactca gagacttaat tcccctatcc
      961 catagataac gataggggaa aattattttt gcagaattta tgttattgcc attatttgag
     1021 caacttttgc agcaggtcca tcacttgctg cacctcttcc tgagtgagcg ccccgtcttt
     1081 ggcccattgc acgcgaccgt ctttatccag caccactaca gcggaacttt cttcatccag
     1141 ctgccaggca ccgcgcgcga cgccattgct atcgacaata aactgcgacc agggataaag
     1201 ctttttatta ctctccagac tgctgcgcac aaacatgccg gaacccggaa tcgcgtcgtc
     1261 ggtgttaaca atggtggtgg tctggtaacg atcgtgcggt aacttcgctg atttaatcgc
     1321 ttcaatcagc gtcgcgtttt tctcttttgc agaggtgcga ccagcaatat gttgcagtac
     1381 tcgcactttt cccactaact gcgcgctgtt ccaggttttg tagctaaact gatctttatc
     1441 aagcaccaac tcgccccgat cggtaatgcc aatcggcggc actcgctgac cggtttcgaa
     1501 ctgatgtgcg gaagccatca tcggcaacag caggcaggtg agtgccagaa tcttgcgtag
     1561 ggtcatggtg tttccttctt atgatatgca ggtgatccga ccacttgggt ctgagtttta
     1621 atcatatgtg ctatttatcg aatttcccgc aagtgtgatg ccagtttgcg gtcaagcgca
     1681 caaatcatat gaaaaatgaa tgcttatact gaagaccgcg cttcggtaaa aagataattc
     1741 tgaataattg taacctttag gtaaaaaaag ttatacgcgg tggaaacatt gcccggatag
     1801 tctatagtca ctaagcatta aaatttgcgc ctcataatag ttgggccgat tgtggcaccg
     1861 cacaggcgta atactcagca ggagataaca atgaaaattt tccaacggta caacccactt
     1921 caggtggcga agtacgtaaa gatcctgttc cgtggacgat tatacatcaa ggacgttggc
     1981 gcttttgaat tcgataaggg taagattctt atcccaaaag tgaaggataa gctgcatttg
     2041 tcagtgatgt ccgaagttaa ccgtcaggtt atgcgtctgc aaacagagat ggcttaacca
     2101 aagtgctatg cagtaaaaag tgctatgcag taaaaagtgc tatgcagtaa aaagtgctat
     2161 gcagtaataa gacggctcct gattcaggag ccgttgatgt ttatgggggt tacgcgacgc
     2221 tttcttcttt atctttagcg tcaggcagtt ttggcgaaag ggctgtggcc ttgctgtcga
     2281 tccgggtcac cagcagctgg tcgatgcggt agttatcgat atccaccact tcaaatttgt
     2341 agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt acgcagcata aacatcataa
     2401 agccgccgat ggtttcgtag ttgcccgact gcgggaactc gtcaatatcc agcacgcgca
     2461 tgacgtcgtc aatcggagtg ccgccgtcaa tcagccatga gttctcatca cgggcgacaa
     2521 tctgctcttc cagtccctga ccgaccagat cgcccatcag cgtggtcatc acgtcgttga
     2581 gggtgatgat ccccactacc agcgcgtact cgttcatgat caccgcgaag tcttctcctg
     2641 cggttttaaa actttccaac gcctctgaaa gggttaacgt atccggcaca atcagcgtgt
     2701 tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt agccagcacg cggttcagca
     2761 ggtctttaga atcgacatag ccgatgatgt ggtcaatatc ttcattacag acgaggaact
     2821 tagagtgcgg atgttccgcc accttatttt tcagactttg ctcatcttcg tggagatcaa
     2881 accaaatcac gttttcacgc ggtgtcattg aagacggaac ggtacgggat tccagctcaa
     2941 agacgttttc aatcagctcg tgttcctgtt tacgtaacac gcccgccagc gcaccggctt
     3001 ccactaccgc gtagatgtca tcagaagtga tgtcatcttt acgtaccatt ggcagtttga
     3061 aaatacggaa gattatgttc gccaggccgt tgaagaacca caccagcggg gtgcaaacgt
     3121 acaggcagaa gcgcatcggg ttgatgatac gcaaagccac agcttctggc gcaatcatac
     3181 cgatgcgttt cggggttaaa tccgcaaaca ggataaacat gccagtcact aacgagaaag
     3241 agagaataaa gctcagttgc tcagagagct ctgccgacat atagcgggag aacaggctgt
     3301 gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc gagaatcgcc actgcgttca
     3361 gaccgatttg gaccacggta aagaacatgc cgggattttc ctgcatattc agaacgcgtt
     3421 gggcatttat attgccttca tcagccagca gtttaagttt gattttgcgt gaggcggcaa
     3481 gtgagatctc agacatcgag aagaacgcac ttacagcgat caagcagagt atgactaaaa
     3541 tactgtttaa catatcttat ccgacctttc aggccagatc ctcggaaggg gaagttgatt
     3601 aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa gtgaaccggc attttcagtg
     3661 gctagtatag cgtaaggtac tgtaaagccg ccagagggtt aaaattcaga cagctgaaaa
     3721 atgcaaaact gcctgatgcg ctatgccgat caggcctacg cagtccttgc aatatattga
     3781 atttggacga ttttgtaggc ctggcaaggc gtttacgccg catccggcat aaacaaaacg
     3841 cactttatca acaatctaca tttgtacccg taacgctatg cttccggtgg cagacagacg
     3901 ccaattccgc caattccaca gtaaccgtac gggtttttat gcagatattg ctggtggtca
     3961 tcttcggcat aataaaacgg tgtggcgtta gcgatttccg tggtgatgtg acgatcgtca
     4021 tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg cgcgagctgc ggcatcctgt
     4081 tctggggtca gcggataaat cgctgaacga tactgcgtgc cgtggtcatt gccctgacgc
     4141 attccctggg cgggatcgtg attctcccaa aatacctgta gcaactgctc atagctgatg
     4201 acggaaggat cgtaaacaat gcgtaccgct tcggcatgac cagtatcacc ggagcacact
     4261 tcccgataag tcggatttgg cgtatagccg ccggtataac ctgcggcggt gctgtaaacg
     4321 ccgtgtaact gccagaacag acgctccaca ccccagaaac aacccatcgc aaaaatggca
     4381 atctccattc cgtcaggtac attggtcatt gagtgaccgt taaccgcatg cagcgtggct
     4441 acgggcatcg gggtgttacg tccaggcagg gcatcggcgg gggaaaccag atgcttttta
     4501 tcaaataaac tcatggtgtc gctctcccga aaatcggtca ttggggttaa ggttgtaaca
     4561 agaggcgtat ttgcacacaa taaccaccgt gaataggtct aaagtaaaac ataagaaata
     4621 tttggggttt agtctgcttt ttaatccata ttactggatt tttgttaagc cgtttaacgg
     4681 cgttccaggg gcaggaaaaa aggatattca ggagaaaatg tgcgctatat ccgacagtta
     4741 tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg ccgcgaacgt ccgtctacag
     4801 gtcgaggggt tatcgggaca gctggaaaag aacgttcgtg cgcagctttc tacgattgaa
     4861 agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg tcgatgatgc tatccgcgaa
     4921 ggtctgaaag cgctgggtta ttaccagccg accattgaat ttgatctccg tccaccgcca
     4981 aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag gcgtgccggt gttaattggc
     5041 ggcaccgatg tggtattgcg cgggggcgcg cggaccgata aagactattt gaaattgctc
     5101 gatactcgcc cggctattgg cacggtactg aaccagggcg attatgaaaa tttcaaaaag
     5161 tccttaacca gcattgcgtt gcgtaaaggt tatttcgata gcgaatttac caaagcgcag
     5221 ctgggcattg cgctcggcct gcataaagcc ttctgggata ttgattataa cagtggcgaa
     5281 cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa tccgcgatga atacctgcaa
     5341 aatctggtgc cgtttaaaga gggcgatgag tacgaatcga aagatctggc agaactgaac
     5401 cgccgacttt ctgctaccgg ctggtttaac tcggtggtgg tggctccaca atttgataaa
     5461 gcgcgcgaaa cgaaagtatt accattgacg ggcgtggttt cgccgcgaac agaaaacact
     5521 atcgaaaccg gggtcggtta ctctacggac gtgggaccgc gcgtgaaagc gacgtggaaa
     5581 aaaccgtgga tgaactcata cggtcacagt ctgaccacca gtaccagtat ttccgcgccg
     5641 gaacagaccc tcgacttcag ctataaaatg ccgctgctga agaatccact ggaacaatat
     5701 tatttggtgc agggcggttt taagcgcact gacctgaacg ataccgagtc tgactccact
     5761 acgctggtgg cttctcgcta ctgggatctc tccagcggct ggcagcgtgc cattaacctg
     5821 cgctggagtc tcgaccactt tactcagggt gaaattacca acaccacgat gctgttttat
     5881 cctggggtga tgattagccg cacgcgttct cgtggtggcc tgatgccaac ctggggcgac
     5941 tcgcaacgct actctatcga ctactccaac acggcctggg gttcagatgt cgatttctcc
     6001 gttttccagg cgcagaacgt ctggatccgc acactgtacg atcgccatcg ttttgtgaca
     6061 cgcggcacgc tgggctggat tgaaaccggt gatttcgaca aagtaccgcc ggatctgcgt
     6121 ttcttcgccg ggggcgatcg cagtattcgc ggctacaaat acaaatctat cgccccgaaa
     6181 tacgctaacg gtgacctgaa aggggcctcg aagttgataa ccggatcgct ggaataccag
     6241 tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg atagtggcga agcggtaagc
     6301 gatattcgcc gcagcgactt taaaaccggt accggggtcg gcgtacgctg ggaatcgccg
     6361 gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg ataaagacga acacgggtta
     6421 cagttttaca tcggtctggg gccagaatta tgagtttatg gaaaaaaatc agcctcggcg
     6481 tggttatcgt tatcttactg ttgctgggat cggtggcgtt tctggtgggc accaccagcg
     6541 gcctgcatct ggtatttaaa gcggcggatc gctgggtgcc aggactggat attggcaagg
     6601 tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg ttatgagcag ccaggcgtgg
     6661 cggtaaaagc gggtaatctg catctggcgg tcggtcttga gtgcctgtgg aacagcagtg
     6721 tttgtattaa tgacttggcg ctgaaagaca ttcaggtcaa catcgacagt aaaaaaatgc
     6781 ctccttctga acaggttgaa gaagaggaag atagcggtcc gctggatctc tccacgccgt
     6841 atcccatcac cctgacacgg gtggcactgg acaacgtcaa catcaagatt gatgacacca
     6901 cggtatcggt gatggacttc acctccggcc tgaactggca ggagaaaacc ctgaccctga
     6961 aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa agtggcggaa gtggcgcagg
     7021 aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga ggaaaagcct ctcggcgaaa
     7081 cactcaaaga tctcttttct cgcccggtat tgccggaaat gaccgacgtg catctgccac
     7141 tcaacctgaa cattgaagag tttaagggag agcaactgcg cgtgacgggc gatacggaca
     7201 ttaccgtgcg caccatgctg ctgaaagtga gcagcattga cggcaatact aaactggacg
     7261 ccctggatat cgattccaat caagggatcc tcaacgccag cggcacggcg cagctgtcag
     7321 acaactggcc ggtggatatc accctcaaca gcacactgaa cgtggagccg ttgaaaggtg
     7381 aaaaggtgaa gctgaaagtg ggcggcgcgc tgcgcgaaca gctggagatt ggcgttaatc
     7441 tttccggtcc ggtggatatg gatttacgcg cccagacgcg actggcggaa gccggattgc
     7501 cgctcaacgt ggaagtgaac agcaaacaga tttactggcc gttcactggt gagaagcagt
     7561 atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat gaccgattac acgctctcta
     7621 tgcgtacggc agtgaaggga ctggagatcc cgccagccac cattaccctt gatgccaaag
     7681 gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc ggcgctggaa gggaaaactg
     7741 aactcaaggc gttgcttgac tggcagcagg ccattagttg gcgcggtgag ctaacgctta
     7801 acggcattaa caccgccaaa gagatcccag agtggccgtc gaaactcaat ggcttgatta
     7861 aaacccgcgg tagcctgtac ggcggcacct ggcagatgga ggtgccagaa ctgaagctta
     7921 ccggtaacgt caaacagaac aaagtgaacg ttgacggcac gctgaaaggc aacagttata
     7981 tgcagtggat gatcccaggg cttcatctgg aactggggcc aaacagtgcc gaagtgaaag
     8041 gcgagctggg ggtaaaagat ctcaatcttg atgccaccat caacgcgccg gggctggata
     8101 acgcgctgcc ggggcttggc ggtacagcga aagggctggt gaaagttcgc ggcacggtgg
     8161 aagcgccaca actactggcg gacatcaccg cgcgcggcct gcgctggcag gagctaaccg
     8221 tggcgcaggt tcgcgtggaa ggcgatatca aatccaccga tcagattgcg ggtaaactcg
     8281 acgtgcgcgt tgagcaaatt tcacagccgg atgtaaatat caacctcgtc accctgaatg
     8341 ccaaaggcag cgaaaaacag cacgagttac agttgcggat tcagggcgag ccggtttccg
     8401 ggcagcttaa tctggcagga agttttgatc gcaaagaaga acgctggaag ggaactctta
     8461 gcaatacccg cttccagacg ctggtcggcc cgtggtcgct gacccgcgat attgcgctgg
     8521 attaccgcaa taaggagcaa aaaatcagca tcgggccaca ctgctggctt aacccgaatg
     8581 cggaactgtg cgtgccgcaa actatcgatg cgggggccga agggcgtgcg gtggtgaatc
     8641 tcaaccgctt cgaccttgcc atgctgaaac cgtttatgcc agaaaccact caggccagcg
     8701 gtatcttcac gggtaaagcg gacgttgcct gggacaccac cgaagagggg ctgccgcagg
     8761 gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca aaccgtcaac gatgcggcgc
     8821 tgcccgtggc gtttcagaca ctgaatctga cggcggaatt gcgtaacaac cgtgccgaat
     8881 tgggctggac catccgcctg accaataacg gccagtttga tggacaggtg caggtgaccg
     8941 atccgcaagg ccgccgtaat cttggtggca acgtcaatat ccgtaacttc aaccttgcga
     9001 tgataaaccc catctttacc cgtggggaaa aagcagcggg gatggtgagt gccaacttgc
     9061 gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca gcttcaggtt acgggtgtgg
     9121 atatcgatgg caactttatg ccgtttgata tgcaaccgag ccagcttgcg gtcaacttta
     9181 acggtatgcg ctcgacgctt gccggtacag tacgaaccca gcagggtgaa atctacctga
     9241 acggtgatgc cgactggagc caaattgaaa actggcgggc gcgggtaacg gcgaaaggca
     9301 gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga tgtatcgcca gatgttgtat
     9361 tcgaggctac accaaacctg tttaccctcg atggtcgcgt ggatgtccct tgggcgcgca
     9421 tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc cagcgatgtg gtgatgctta
     9481 acgataacct gcaaccggaa gagccgaaaa cggcgtcgat tccgattaac agtaacctga
     9541 ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg cctgaaagcg cggctgacgg
     9601 gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct gaacgggcag atcaacatcc
     9661 ctgaagggcg cttccatgcc tatggtcagg atctgattgt gcgtaaaggt gagttactgt
     9721 tctctggccc gccggatcaa ccgtatctta atattgaagc tattcgtaac ccggatgcta
     9781 cagaagacga cgtaatcgcc ggagttcgcg tcactggtct ggcggacgaa ccgaaagcgg
     9841 agctcttctc tgacccggcg atgtcgcaac aagctgcctt gtcttatttg ctacgtggac
     9901 aagggctgga gagcgatcag agcgacagtg cggcaatgac ctcgatgctg attggtctgg
     9961 gggttgcgca aagtggccag attgtaggta aaatcggcga gacgtttggc gtaagcaatt
    10021 tagcgctcga cacccaggga gtaggcgact cctcccaggt agtggtcagc ggctatgtat
    10081 tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga ctctatagca acactcacgt
    10141 tacgttatcg cctgatgcct aagctatatc tggaagccgt gtctggtgta gaccaggcac
    10201 tggatttgct ctatcagttc gagttttagc aatgcgaata tttgtctacg gcagtttacg
    10261 ccacaaacaa ggcaacagtc actggatgac caatgcccag ttactgggcg atttcagtat
    10321 cgataactac cagttgtata gcctgggcca ctatccaggc gcagttccgg ggaacggaac
    10381 ggtacacggt gaagtttatc gtattgacaa cgccacgctg gccgaacttg atgccttgcg
    10441 caccaggggc ggtgaatacg cgcgccagtt gattcagacg ccgtacggga gtgcatggat
    10501 gtacgtttat caacgacccg tcgatggatt aaagctaatt gaaagcggcg actggttaga
    10561 cagggataag taaccctatg catacgccac cttcgggtgg cgttgttttt tgcgagacga
    10621 ctcgcattct gttttgtaat tccctcacct tttgcttttc tctccgagcc gctttccata
    10681 tctattaacg cataaaaaac tctgctggca ttcacaaatg cgcaggggta aaacgtttcc
    10741 tgtagcaccg tgagttatac tttgtataac ttaaggaggt gcagatgcgt attaccataa
    10801 aaagatgggg gaacagtgca ggtatggtca ttcccaatat cgtaatgaaa gaacttaact
    10861 tacagccggg gcagagcgtg gaagtgcagg tgagcaacaa ccaactgatt ctgacaccca
    10921 tctccaggcg ctactcgctt gatgaactgc tggcacagtg tgacatgaac gccgcggaac
    10981 ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg tgacgaaata tggtaaagaa
    11041 aagtgaattt gaacggggag acattgtgct ggttggcttt gatccagcaa gcggccatga
    11101 acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt caagccttta atcaactggg
    11161 aatgacgctg gtggccccca ttacgcaggg tggaaatttt gcccgttatg ccggatttag
    11221 cgttccttta cattgcgaag aaggcgatgt gcacggcgtg gtgctggtta atcaggtgcg
    11281 gatgatggat ctacgcgccc ggctggcaaa gcgtattggt ctggctgcgg atgaggtggt
    11341 ggaagaggcg ttattacgct tgcaggcggt ggtggaataa ggtgtgttta tttatcgcgg
    11401 gcataaaaaa acccttacta accgaagccc ggcgttcagg gttattacgc cagaagaact
    11461 tatttattct ttgcgcgctc gaaggaggca acgatttcag ctttagcggc ttctgcgttt
    11521 tcccaacctt caactttcac ccacttgcct ttttcgaggt ctttgtagtg ctcgaagaag
    11581 tgagcgattt gcgctttcag cagttcaggc agatcgttaa cgtctttaat gtgatcgtat
    11641 tctttgctca gcttgctgtg cggaaccgca accagtttcg catcttcacc ggcttcgtcg
    11701 gtcattttca gaacgccaac cggacggcaa cggatcacag aacccggctg cagcgggtac
    11761 ggagttggga ccagtacgtc aactgggtca ccgtccagag acagggtgtg gttgatgtaa
    11821 ccgtagttgc acggatagaa catcgcggtg gacatgaagc ggtcaacgaa cagtgcgccg
    11881 ctctctttgt cgatttcgta tttgatcgga tctgcgttag ccgggatctc aataacaacg
    11941 tagatgtctt ccggcagatc tttacccgca gggacgttga gtaagctcat gtctgtttcc
    12001 tttaaaaata tgtggcaaac aagtgccgag tattatagcc aactcgcgcc gaatgtcttc
    12061 gcttgttttc gtcttcattt tcctttttcg ccagttttaa gacggtatgc agagcaggaa
    12121 aatccataac cgtagccgca tttttcatag tgagatgaaa gcgattacaa acttgtgatt
    12181 aacgttttat ttactttttt gaagtgtgat gtaacgcaat ccgttacata acgaattgtc
    12241 tatagttttt tcgcgaacat cttttcacca ataataatta ctccgacgag gacaacccta
    12301 tgtggaaacg cttacttgta gtctctgcag tctcggcagc catgtcgtct atggcgttgg
    12361 ccgctccatt aaccgtagga ttttcgcagg tcggatcgga atccggctgg cgcgccgcag
    12421 aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat tacgctgaaa attgccgatg
    12481 gtcagcaaaa gcaggaaaac cagattaaag cggtacgttc cttcgttgca caaggggtgg
    12541 atgcgatctt tatcgctccg gtggtcgcga caggttggga accggtatta aaagaggcga
    12601 aagatgccga aatcccggtc ttcttgctcg accgttccat cgatgtgaaa gacaaatctc
    12661 tctatatgac caccgttact gccgacaaca tcctcgaagg taagttgatc ggtgactggc
    12721 tggtaaaaga agtgaatggc aaaccatgca acgtggtgga gctgcagggc accgttgggg
    12781 ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc cattaagaat gcgccaaata
    12841 tcaaaatcat ccgctcgcag tcaggtgact tcacccgcag taaaggcaaa gaagtcatgg
    12901 agagctttat caaagcggaa aacaacggca aaaacatctg catggtttac gcccataacg
    12961 acgacatggt gattggtgca attcaggcaa ttaaagaagc gggcctgaaa ccgggcaaag
    13021 atatcctcac gggttccatt gacggtgtac cggacatcta caaagcgatg atggatggcg
    13081 aagcgaacgc cagtgttgaa ctgacgccga acatggcggg acccgctttc gatgcgctgg
    13141 agaaatacaa aaaagacggc accatgcctg aaaagctgac gttaaccaaa tccacccttt
    13201 acctgcctga taccgcaaaa gaagagttag agaagaagaa aaatatgggg tattgagggt
    13261 tgctatgcct gatgccgatt cgtaggccgg ataaggcgct cgcgccgcat ccggcgatgg
    13321 tgcactgaag cctgatgcga cgcttaccgc gtcttatcat gcctactggg agcacgcttt
    13381 acaccggggg aaaccatgac gaccgaccaa caccaggaga tcctccgcac cgaaggatta
    13441 agtaaatttt tccccggcgt caaagcgtta gacaacgttg atttcagcct gcgccgtggc
    13501 gaaatcatgg cgctgctcgg tgaaaacggg gcgggaaaat caacgctaat caaagcatta
    13561 actggtgtat accacgctga tcgcggcacc atctggctgg aaggccaggc tatctcaccg
    13621 aaaaataccg cccacgcgca acaactcggc attggcaccg tctatcagga agtcaacctg
    13681 ctacccaata tgtcggtcgc tgataatcta tttataggcc gcgaacccaa acgcttcggc
    13741 cttctacgcc gcaaagagat ggaaaagcgc gccaccgaac tgatggcatc ttacggtttc
    13801 tccctcgacg tgcgcgaacc gctcaaccgc ttttcagtcg cgatgcagca aatcgtcgct
    13861 atttgccggg ctatcgatct ctctgccaaa gtgctgatcc tcgatgaacc caccgccagt
    13921 ctcgacaccc aggaagtgga gttactgttt gacctgatgc gtcagttgcg cgatcgcggc
    13981 gtcagcctga tctttgtcac tcactttctc gatcaggtct atcaggtcag cgatcggatc
    14041 accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa cgtgcgagct accgcagatc
    14101 gaactggtaa aaatgatgct ggggcgcgag ctggataccc acgcgctaca gcgtgccggg
    14161 cgaacattgt tgagcgacaa acccgttgcc gcgttcaaaa attacggcaa aaaaggaacg
    14221 atcgcaccgt ttgatctcga agtacgcccc ggcgagatcg tcggtctggc tggattgctg
    14281 ggatcaggac gtaccgaaac cgccgaagtg atcttcggta tcaaacctgc tgacagcggc
    14341 acggcgttga tcaaaggcaa accgcaaaac ctgcgatcgc cacatcaggc ttcggtactt
    14401 ggcattggct tctgcccgga agacaggaaa accgatggca tcatcgctgc cgcctcggtg
    14461 cgggaaaata tcatcctcgc tctccaggcc cggcgcggct ggctacgtcc catttcccgc
    14521 aaagaacagc aagagattgc cgaacgcttt atccgccagc ttggcattcg cacaccttca
    14581 actgaacaac cgattgaatt tctctccggc ggcaatcagc aaaaagtgtt gctttcacgt
    14641 tggctactga cccgaccgca atttctgatc ctcgatgagc caacccgcgg cattgatgtt
    14701 ggtgcccacg ccgagatcat ccgcctgatt gaaacgctat gcgccgatgg tctggcgctg
    14761 ctggtgatct cctccgaact ggaagaactg gtgggctatg ccgaccgggt gatcatcatg
    14821 cgcgatcgca aacaggtggc ggagatcccg ctggcagagc tttccgttcc ggcgatcatg
    14881 aatgccattg cggcgtaagg agaacagtgt gatgcctcaa tctctcccgg acactacgcc
    14941 gccaaaaagg cgctttcgct ggccaacggg aatgccgcag ctggcggcac tgttgctggt
    15001 gctgctggtc gatagcctgg tggccccgca tttctggcag gtggtgcttc aggatgggcg
    15061 tttgttcggt agccctatcg acattcttaa tcgcgccgcc cccgttgcgc tactggcgat
    15121 tggtatgacg ctggtgatcg caacaggtgg gattgatctc tccgtagggg cggtgatggc
    15181 tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga ttcagcctgc cgattgtttt
    15241 gttaagcgcc ctgggcaccg gcatcctggc gggattgtgg aacggcatac tggtagcgat
    15301 cctcaaaatt cagccgtttg tcgccactct gatcctgatg gtcgccgggc gcggcgtggc
    15361 gcaactgatc acctccggac agatcatcac gtttaactcg ccggatctct catggttcgg
    15421 cagtggatcg ctgttgttcc tgccaacgcc ggtcattatc gcggtgctga cgcttatcct
    15481 gttctggctg ttgacccgca aaacggcgct ggggatgttt atcgaagccg ttggtatcaa
    15541 cattcgggcg gcaaaaaatg ccggggtaaa cacgcggatc atcgtcatgc ttacctacgt
    15601 gttgagcggg ctgtgtgcgg cgattgcggg cattatcgtg gcggcggata ttcgcggtgc
    15661 cgatgccaac aacgccggat tatggctgga gctggacgcc attctcgcgg tggtgattgg
    15721 cggtggttcg ctgatgggcg gacgttttaa cctactgctt tcggtggtgg gggcgctgat
    15781 tattcagggg atgaacaccg gaattttgct ttcgggcttt ccgccagaga tgaaccaggt
    15841 ggtgaaagcg gtggtggtgc tttgcgtgct gattgtccag tcgcaacgct ttatcagtct
    15901 gattaaagga gtgcgtagcc gtgataaaac gtaatttgcc actgatgatc accatcggcg
    15961 tctttgtgct gggttatctc tactgcctga cccagtttcc cggttttgct tccacaagag
    16021 tgatctgcaa tatcctgacc gataatgcct ttcttgggat aatcgccgtt ggcatgacct
    16081 ttgtgatcct ctccggtggg atcgatctct ccgttggttc ggtgatcgcc tttaccggcg
    16141 tgtttctggc aaaagtgatt ggcgatttcg gcctctcgcc gttgctggca tttccgctgg
    16201 tgctggtaat gggctgtgcc tttggcgcat ttatggggct tttgatcgac gccctgaaga
    16261 tcccggcatt tatcattact ttggcaggaa tgttcttttt gcgcggcgtc agctacctcg
    16321 tttcggaaga gtcgatcccg ataaaccatc ctgtttatga cacgctctca agccttgcat
    16381 ggaaaattcc tggcggcggt cgcttaagtg cgatggggct gctgatgctg gcggtggtgg
    16441 ttatcggcat attcctcgcg catcgtaccc gctttggtaa tcaggtgtac gccattggcg
    16501 gcaatgcgac gtcggcaaac ctgatgggga tttccactcg cagcacaact attcgcattt
    16561 atatgctctc taccggactg gcgacactgg cggggattgt cttctcgatt tatacccagg
    16621 ccggatacgc gctggcgggc gtaggtgtgg aactggacgc tatcgcctca gtggtgattg
    16681 gcggtacgct tttgagcggt ggcgttggaa cggtattagg gacgcttttt ggcgtggcga
    16741 ttcagggact gattcagact tacataaact ttgatggtac gctgagctca tggtggacga
    16801 aaatcgccat cggtattttg ttgtttattt ttatagcatt acagcgtgga ttaacggtgc
    16861 tgtgggagaa tcgtcagagt tcgcctgtga caagagtcaa cattgcgcag caataaaatc
    16921 gcctctccgt gtggagaggc gcaggagatt acgcgtccgg gaactcacag ataaagcgtt
    16981 cgacatcttc aaccatatgg tcgttgccga caaagaatga acggcgctgg tgcagggttt
    17041 ccgggataat atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg
    17101 ccaggaatgc catcgggttg cactcataca gcaaacgcag tttgccgtcc gggtggctgg
    17161 cggtgcttgg gtagagataa ataccgcctt tcagtaggtt acggtggaaa tccgcaacca
    17221 gtgagccgat ataacgtgag gtatacgggc ggttggtgga tttatcttct tcctggcaga
    17281 atttaatgta cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt
    17341 aggttttgcc tttctccggg aagcgcatcc gttcctggca caggcagaaa acgccgagcg
    17401 aaggatcgta agtaaaagcg tgaacaccgc atccggtact gtaaaccagc atggtagagg
    17461 agccgtatac cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt
    17521 ccgttaccgg cgtgccgacg ggcgtaactc ggcggtagat ggagaaaatg gtaccgacag
    17581 agacgttaac atcgatgttg gacgagccat ccagtgggtc catcagcacc acgtattttg
    17641 catgttcaca gccttcaaag acaacaatct catcttcttc ttcagaggca atgcctgcaa
    17701 cgatatcgcg tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt
    17761 gctgaacctc gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg
    17821 ctttgttgat atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag
    17881 tgagctcacc ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg
    17941 ttttcataaa actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa
    18001 ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa ttacggatta tcctgaaatg
    18061 cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac
    18121 gcatgcgcat tcatatttta ggaatttgtg gcacgtttat gggcggtctg gcgatgctgg
    18181 cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca
    18241 ccttacttga gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgacc
    18301 cgcagccgga tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag
    18361 cggtactgga aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc
    18421 tgcgcgaccg ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa
    18481 tggcgacttg gattctggaa cagtgtggtt acaaaccggg ctttgtaatc ggcggtgtgc
    18541 cggggaactt tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg
    18601 atgagtacga ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta
    18661 cgctgatcct caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga
    18721 tccagaaaca gttccaccat ctggtgcgta tcgttccggg gcagggtcgt attatctggc
    18781 cggaaaatga catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc
    18841 tggtgggtga gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg
    18901 aagttttgct ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata
    18961 atatgcacaa tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag
    19021 atgccgctaa cgcgctgggt tcgtttatta acgctcgccg ccgtctggag ttgcgtggtg
    19081 aagcgaatgg cgtcacggta tatgacgatt ttgcccatca tccgacggcg attctggcaa
    19141 cgctggcggc gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggagc
    19201 cgcgctcgaa taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc
    19261 gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg
    19321 cagaagcctg cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg
    19381 tggtgaaaac cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg
    19441 ggatccatca gaaactactg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg
    19501 gcctcagcct gagatagtgt gcgtaggccg gataaggcgt tcacgccgca tccggcattt
    19561 gagcatagtg cctgatgcga cgcttgatgc gtcttatcag gcctacaggt gcaccgcatc
    19621 cggcaaacca tactcacatc aacaacgaaa attacccttc attctccgcc agttcgcgca
    19681 gatactggaa aatctggcgt gcggatttcg gcggcttatt cccttctttc tctttcttcg
    19741 cgttgcggat cagagtacgc agttgctgac gatcggcatc gggccacaga tttaatacct
    19801 cagcgatggc gtcatcacct tgatcgatca gacgatcgcg caggttttcc agtttatgga
    19861 acagcaccac ctgctggttg tgacggtttt tcagcttatc cagcgcctga cggatcggtt
    19921 ctacatcgcg ctggcgcagc atcttaccga tgagctgcaa ctggcggcgg cgaccttcca
    19981 tcttaatacg ctgggcgagc tcaatagccg cgcgcagatc cgcatctaac gggatcttat
    20041 ccagcgcgtt tttccccaga tcaacaattt ccgcgccaag gcgttttagc tcctcggcat
    20101 cacgtttaat ttcactttta ctgacccaga taatttcatc gtcttcgtct tcgatgtcat
    20161 caccgggaac gtcgtcgagc cagtcttcgg gctgcttagt catctcaggc tccttaaaaa
    20221 aagaggctaa tgttaccagt taagatgcgc actgaaaaac ggttctctgt tagacttcag
    20281 agaaactctc tacattatgg cacttgcaat gaaagtaatc tctcaagttg aagcgcagcg
    20341 caagattctg gaagaagcag tttccactgc gctggagttg gcctcaggca aatcggacgg
    20401 tgcggaagtt gccgtcagca agaccaccgg cattagcgta agcacgcgtt atggtgaagt
    20461 ggagaatgtt gaattcaata gcgatggcgc gctggggatc actgtttatc accagaaccg
    20521 caaaggtagc gcatcatcca ccgatttaag cccgcaggcc attgcccgta ctgtacaggc
    20581 ggcgctggat attgcccgtt atacctcgcc agatccctgt gccggcgtgg cagacaaaga
    20641 gctgctggcc tttgacgcac cggatctcga cttgttccac cctgcggaag tttccccgga
    20701 tgaagccatt gaactggcgg cccgcgcaga acaggcggca ttgcaggcgg acaaacgcat
    20761 caccaatacc gaaggtggca gctttaacag ccactacggt gtcaaagttt ttggcaacag
    20821 ccacggcatg ttgcagggtt actgctcaac gcgtcattcg ctctccagct gtgtaattgc
    20881 cgaagaaaat ggcgatatgg agcgtgatta cgcctacacc attggccgcg cgatgagcga
    20941 tttgcagacg ccagagtggg tcggggccga ttgcgctcgc cgtactttat cgcgtctgtc
    21001 accgcgtaaa ctctccacca tgaaagcgcc ggtcattttt gccaatgaag tggcaaccgg
    21061 gctttttggg catctggtgg gggcgatagc gggtggatcg gtttaccgta aatctacctt
    21121 cctgctggat tcgctgggta aacaaattct gccggactgg ctgaccattg aagagcatcc
    21181 gcatctgctg aaagggctgg catcgacgcc atttgacagc gaaggtgtgc gcaccgagcg
    21241 tcgcgatatt attaaagatg gcatcctgac tcagtggctg ctgaccagct actcggcgcg
    21301 gaaactgggg ctgaaaagca ccggacatgc gggcggtatt cacaactggc ggattgccgg
    21361 acaaggtcta agcttcgagc agatgctcaa agagatgggc accgggctgg tggtgacgga
    21421 attgatgggc cagggcgtga gtgcaattac cggtgattat tcccgtggtg cggcgggctt
    21481 ctgggtagaa aacggcgaaa ttcagtatcc ggttagcgaa atcaccatcg caggtaattt
    21541 aaaagatatg tggcgcaata ttgtcaccgt cggtaacgat attgaaacac gcagtaatat
    21601 acagtgtggt tctgtgctgt tgccggagat gaaaatcgcc ggacagtaaa aaaactggcg
    21661 cgacctgccg cgctataaac taaaaaatta cacaaaatca ttcgcactgc atcgagacgg
    21721 aaagtgaatg agggccgggg agcgtactcg cagtacgtga cccggtcgaa tgagcgtagt
    21781 cgtcgatgag gcagtgtgaa ggatgaagtg taaataaaaa aggaagtgag caatgcgaaa
    21841 aagcctgtta gctattcttg cagtctcctc gttggtattc agttcggcgt cgtttgctgc
    21901 tgatcttgaa gacaatatgg aaaccctcaa cgacaattta aaagtggtcg aaaaagccga
    21961 taacgcggcg caagtcaaag acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca
    22021 aaaagcaacg ccgccgaagc tcgaagataa atcaccggac agcccggaaa tgaaagattt
    22081 ccgccacggt ttcgacattc tggtcggtca gattgacgac gcgctgaagc tggcaaatga
    22141 aggtaaagta aaagaagcgc aggctgctgc agagcaactg aaaacgaccc gcaattcata
    22201 tcacaagaag tatcgttaat tcctcatttc cctgttgcct gcactcaggt aacagggaac
    22261 ttatcatcgc aaatgatgca ccacctgatt gctgctgccg cgccagatca gggaagggtc
    22321 ttttaaatcc tgcacaaatt tgccgtcgac cagcacgtta atcaaatcaa caacctgcat
    22381 ttgcgcagcg ttgagttcgt cgagtttata gcctgtccac acccagatgt ctttacccgg
    22441 acactcggcg cggatgcgtt gtaccagttt cagaatatcc ggcacgtttt gcggatgcag
    22501 cggatcgccg ccggagaggg aaataccctg gcgtttgata cgagtgtcat tcagatcgtt
    22561 aatgatctgg tcttccattg ctttagtaaa tggctgaccg gaatttaccc gccatgtgct
    22621 tttgttatag caaccggggc attcatgaac acacccggag acaaacaggg tgcagcgagt
    22681 gccgggaccg ttgacgatgt cgacaggata gtactgatga taattcatta tttcgcctgg
    22741 cttatcagtc cgcgtgtagg ctgtatcagg caatcaacgc gggatgcgac gctttcacat
    22801 cacatccggc atttgcttag tgcagatcaa cctatctgcc catttcccaa atgtttaacg
    22861 cggcgcttaa cttcttcctg cttgccggcg ttaaacggac gtgcatccgg gctacctaaa
    22921 tatccgcaca cgcggcgagt taccgacaca cgggaggcgt catggttacc acatttcggg
    22981 caggtgaagc ctttgctggt gcactcgaac tcaccggtaa agccacactc gtagcactca
    23041 tcaatcggtg tattggtgcc gtaatacggt acatgctgat agctgtaatc ccagacatct
    23101 tccagcgcct tcaggttgtg ctgaatgttt ggatactcgc cgtagcaaat gaaaccaccg
    23161 ttcgccagcg gcgggtaagg cgcttcaaag tcgatcttgt cgtacgggtt caccttcttc
    23221 tccacatcga ggtggaaact gttggtgtag taacctttat cggtcacgcc cggcaccacg
    23281 ccaaactcag cagtatcgag acggcagaag cggtcgcaca ggttttcact cggcgtgctg
    23341 tagagactga aaccataacc cgtttcttct ttccactgat ccactgcctg acgcagacgt
    23401 tcaacaatgg cgataccttt cgcgcggagc tgctcgttgt cgtaaacatg ttcgccgccg
    23461 aacagcgcgt taatggtttc gtggatgccg atgtaaccca gcgaaataga cgcacgaccg
    23521 tttttgaaga tttcagaaat atcatcgtca gcattgagac gcacgccaca agcaccttcc
    23581 atatagagga tcggggccac gcgcgctttc acgccttcga gacgagcgat acgggtcatc
    23641 agagccttac gtgccagcac cagacgttca tccagcagct tccagaaggt ggcttcatcg
    23701 ccttttgctt ccagagcaat acgcggcagg ttcaggctga tcacgccgag gttgttacga
    23761 ccatcgtgga tctgctcgcc gttttcattt tcccacacgc cgaggaagct gcggcagccc
    23821 atcggggttt taaacgaacc ggtgactttc actacctgat cgtagttcag gatatccgga
    23881 tacatgcgct tgcttgcgca ctccagcgcc agctgtttga tgtcgtagtt cggatcgcct
    23941 tttttatggt tcagaccatc gcgaatcgca aacaccagtt tcgggaacac cgcagtttta
    24001 cggtttttac cgaggcctgc gatacggtta cgcaggatag attcctgaat caggcgcgac
    24061 tcccagctgg tgcccagacc aaaaccaaag gttacaaacg gcgtctgacc gttggcggtg
    24121 tgcagggtgt ttacttcgta ctccagcgac tggaaggcat cgtagcactc tttaatggta
    24181 cgagagttag cgtagccttc ggcgtccggg atgttccact cttctgcggt cttgcgatgt
    24241 ttgttgtagc tggcggtgac aaacggtgcc agcacttcat cgatacggtt aatggtggtg
    24301 ccgccataaa tatggctggc aacctgagca ataatctgcg cagttaccgc ggttgccgta
    24361 gagatcgact tcggcggttc aatctcggcg ttccccatct taaagccctg ggtcagcatg
    24421 cctttcaggt cgatcaacat gcagttgaac atcgggaaaa acggtgagta atcgagatcg
    24481 tgatagtgaa tatcgccacg ctcatgtgcc tgcaccacgt cacgcggcag caggtgctga
    24541 cgtgcatagt gtttagccac gatcccggcc agcaggtcgc gctgggttgg aatcaccttg
    24601 ctgtctttgt tggcgttttc gttgagtaac gaggcgttgg tctgctcgac cagaccacgg
    24661 atctcctgat tcaggcgacc gcgtttttca cgttcaatgt cgcgatcgtg acggtactcg
    24721 atgtaagcac gagccagttg tttgtatgga cctgacatca gctggttttc aacagcggtc
    24781 tggatctcat tgatatccac ctggttgcgg ccctgcatct gctcgctgac aactgcagca
    24841 acagtggcgc aataatcggc atcatcgact tccgctgctt tagctgcacg cagaatcgct
    24901 tctttgatgc gctctgattt aaacggcact ttgcagccgt ctcgtttcat cacatgcggt
    24961 gtcatgatcg ctccatattt ttaagaacag gttatccaca gaaattggga aaggcgtttt
    25021 ccggtttttt cgtttcgttt tccggtgctt tccgcaatcg ccatccgctt atccacaacg
    25081 ccgcaccttt ttcgtgggga gctgtagtag caattatagt cgattgatac aacatattgg
    25141 gttgggacgc attttaaagt ctatatatag tgctttgcat caaggatgtt tgaacttttt
    25201 ttgatgtagc tcaaagtaaa aagcagagcg tacggatgac gggcgctaca gcgttatgta
    25261 aattttttaa tgaatttgct ggttgaaaaa tcaacaaaaa caacatgctg acagacaaaa
    25321 ccccggaatg accggcatta ccggggctta gggaagattt acttctgtaa ccaccagaca
    25381 gcctcaaaag gccgtaaagt catggcacag ggttgtggtg aggcttcttc gtagttatgc
    25441 atcacaagct gccagttgcc gcgcatttgc cctggctgcc agggttggat ctcacggcta
    25501 aggttggcaa tgaccagcaa ggtttgcccc ttccattcac ggcgatagca ccacaataca
    25561 gggctgtttg gcagcagatc ctggtaattg ccccatgtca ggatggcttc ctgcttacgc
    25621 agtgcgatta acttttggta ggtgtaaaac accgaggact catcggccag cgcggcttct
    25681 acgttgattt gttgatagtt atcgcccagg ccaatccacg gttcgccagc cgtaaacccg
    25741 gcattatcgc cgttgctcca ttgcatgggc gtgcggctgt tgtcacggga tttactggcg
    25801 aggattgcca ataactcgtc ggcatcacgc ccatcgttgc gcagctcggc aaacatattg
    25861 aggctctcta cgtcgcgata gtcagtaatg cgcgtgaaat gcgggttggt catgccaatc
    25921 tcttcgccct ggtagatata cggcgttccc tgcatgccat gcagcaccat cgccagcatt
    25981 tttgccgcag gcacgcggta ttcaccttca tcaccaaagc gagaaacaat gcgcggctga
    26041 tcgtggttac accagaacaa ggcattcaat gctacgttgt gcattccttg ttgccagtgg
    26101 cggaacaatg ttttcaacgc cacaaagtca ggtttagcca gcgtccattt ttcaccaccg
    26161 ggataatcga ccttcaggtg atgaaaatta aaggtcatcg acaattcact gcctgtcagt
    26221 gccgcgtatc gctggcaatg ctcaaggctg gtggaggaca tttcacctac ggtcattaac
    26281 ccgcgtggcg taaacacatc gcggttcatc tcgtgcaaaa actcgtgtgc tcgtggcccg
    26341 tcggtgtaga agcgacgccc gtcgccatcc aggtcatcag ggaaacgcgg gtctttggag
    26401 atcagattca ccacatccag gcgcaacccg tcgaccccac gatcggccca gaactcacag
    26461 acttttttca gctctgcgcg taccgctgga ttctcccagt tgagatccgc ctgttctggt
    26521 gcaaagagat gcaaatagta ctgttcgctt tccgcatgcc agcgccacgc actaccgcca
    26581 aattttgaac gccagttgtt cggtggcgtt tctggttcgc catcgcgcca gatataaaac
    26641 tggcggtaag ggctttcttt gttcagcgcc tcgcgaaacc aggcatgttg ggtagaggta
    26701 tggttaaaca ccatatcgag aatgatacga atcccgcgcg attttgcctg cgtcaccagt
    26761 tcgtcaaaat cgtccagcgt gccgtaggtg ggatcaatcg ccgtatagtt cgctacatcg
    26821 taaccgttat cgacctgcgg agagacataa aagggggtta gccagatggc atcaacgccc
    26881 agtttatgca gatagtccag gcgttggata acgccacgta aatcgccggt gccgctaccc
    26941 gtggtgtcct gaaaactctt tggataaatc tggtagataa cgccgttttg ccaccagtgg
    27001 ggaagattag tcattacgtt attcctgcaa atgcgagggg gcgcaattgc gccccgaaga
    27061 aaattaaaca atgtccagcg tgcccaggcg gtatttccgc tgatagataa acgaggtgag
    27121 tacaatcggg atgatgatgg cgatagccat tgccagcgca aacacctgcc agtagctcgg
    27181 ttgaatcgag agaattcccg gcaggccgcc tacgccgatg ccattcgcca taacgccgtt
    27241 caggccgcat agcaatcctg ccagaccaga accaatcatc gcgcacagca tcgggaagcg
    27301 atatttcagg ttgatgccgt acattgcagg ctcagtgacc ccaagccagg cggagatcgc
    27361 cgcaggcacg gagatctcgc gttcattgtg cttgcggctg gaaatgataa tgcctatcac
    27421 ggcggagccc tgagcgatat tcgacagcgc aatcagcggc cacactggcg taccacccat
    27481 gctttgaatc atctgcaaat caatagcaag cgtggtctgg tgtacaccgg tgatcaccag
    27541 cggggcgtac aggaagccaa acaatgccgc gccaatcgga gcaaagctgc cggtcatcag
    27601 gtgacgtacc gcaaaggcaa cgccatcgcc aatcatgcga ccaaacggac caatcagcgc
    27661 atgggcgagg aacaccgcga ggatcagcga acagacgggc accaccacca gatagaggta
    27721 atccggcacg atgcgtttaa ggcgagtttc aataacgccc agcgccagcc cggctaacag
    27781 tgccgggatc acctgcgcct gatagcccac tttggcgatg ctgaacatgc caaagtccca
    27841 cacttccggc agctgctgcc cgagcagata agcgttcatc agttgggggg aaaccagtgt
    27901 cacgccaagc acgataccaa ggatcggcgt gccgcccatt tttttcaccg ctgaccagca
    27961 aatcccgacc ggcaggtaga agaagatcgc ttcaccgatc aaccacagaa aatcgtagat
    28021 cgtttgcagg gaagggtaca tttgcgccag cgtttgaccg ttgctcatgg gcaaatcgcc
    28081 gatcacattg cgaaaaccga ggatcaaacc gccgctaatc aacgcgggca gcaacgggaa
    28141 gaagatctct gcgaaatgag agatcaactg ctcatgccat ttcatattct gccgggcggc
    28201 tttttttacc tgctctttat caacctgcgc ctgtccggtt gacgctatca gtgcttgata
    28261 gtaatcaccc acgttggtgc caatcaccac ctgaaattgc ccggcattgg tgaaacagcc
    28321 tttcaccatg gggagttgct caatttcttt cggtctggca ttggccggtt ggttgaggac
    28381 aaagcgcagg cgagtaatac agtggctcac cgtcgcaata ttgccgcgcc cgccgaccag
    28441 ttcaatcaac cgatcgatat ccgtttggtt tattttgctc atcataaagc cccatggcag
    28501 atgacatttt tggttggctg cagaatatat tgcgcggaaa aatttaaaaa cgggaacgtt
    28561 cccgaaacgc agcgaagatc acaatttatc gttcaggaaa cgatcaggac aggttggcgg
    28621 ggatgatgat ttgttgcggt tcgctgcgtc cggttacctg cgcgatcaac tggcaagccg
    28681 cctggcgtcc agcttcggcg taaccaggat ctacggtgac gatctccgga tgaaggaatt
    28741 tcattaacgg cgtattaccg acgctcgcca gttgcaaggt gtcgatgcgt tgctcttgca
    28801 ggtatttact tgcgccaagt gccagcgtgt cggttgcgca cagtaaggcg gtagtttcag
    28861 gcgtaatcac ttttgctacg ttctcatagc cttgcttcat agcaagccct ggcagggcgg
    28921 caacgggatg cagtttatgc gctttgcaga acgccaggta ggcttcgtga cgtcgcttac
    28981 cggttgtcac gtcactgtgc ggcacgccga gataactgat attacgatgc ccctggtcat
    29041 acagccgttg catcaggatc ttgattgccc cttcgtcgtc ataacagacc gaagcaaagc
    29101 cttttgcgtc acgcgccagc agaaccagcg atgactgcca gtgggctaac atttcttctg
    29161 ttatgccagt aaaaccgaac agcactacgc cgtcgatatt acgccgtttc agcaccccca
    29221 aatgttcggc aactaattgc ggggaaaact gactttccat catgattggg tcgtaacctt
    29281 gttcatagaa cgctggcagc atggtttgaa cggcgagatt ttctgacaac gaatccagac
    29341 gggtaacaat gatggcgacc actttatcgc tttgcccacg catagcgcgc gcagaacggg
    29401 aaggggaaaa tccatgctga ttcatcactg cttcaacacg ctcgcgggtg cgctggctca
    29461 cgccgctttc gttattcagc acccgggaaa ctgtagattt ccccacgccg cttaagcgcg
    29521 cgatgtcttt gatggtcagc cgattttgca tcctgttgtc ctgtaacgtg ttgtttaatt
    29581 atttgagcct aactttaccc gtgcattcag caatgggtaa agtctggttt atcgttggtt
    29641 tagttgccag caggtattat atcgccatag atgctacgaa tattattgga ttctccttat
    29701 tatttgcggc gcttttttca cttaccggag gttatatgga acctgatccc acgcctctcc
    29761 ctcgacggag attaaaactt ttccggtaag cccgtctttt cacggcgtta ccggatgcgt
    29821 aaggccgtga cgttttaacg tccctgctca gctttattac cttcaggtaa ggcttcgcca
    29881 cgcctgaaga catttctgta ctgtttcaga cagtgcggag ggactcctta tgtttaaaga
    29941 aatttttacc cggctcattc gccatttacc ttcccgtctg gttcatcgtg atccattgcc
    30001 tggcgcgcag cagacagtga atacggtggt cccgccgtcc ttaagtgcgc attgcctgaa
    30061 aatggcggtg atgcccgaag aagaattgtg gaaaacgttc gacacccatc cggaagggtt
    30121 aaatcaggcg gaagtggaat ctgcccgcga acaacatggt gaaaataaat tacccgcaca
    30181 acaaccgtcg ccgtggtggg tacatttatg ggtctgctat cgcaacccct ttaatatttt
    30241 actcaccatt ctcggcgcta tttcttacgc cacggaagat ttatttgccg cgggcgttat
    30301 cgcgctaatg gtcgctattt caacgttgct gaactttatt caggaagcac gttccactaa
    30361 agcggcagat gccctgaaag cgatggtcag caataccgcg acggtgctgc gcgtaattaa
    30421 cgacaaaggc gaaaatggct ggctggagat cccgatcgac cagctggtgc ccggcgatat
    30481 tataaaactg gcggcgggag atatgatccc ggcagattta cgtatcttgc aggcgcggga
    30541 tctgttcgtc gctcaggcgt cgttaaccgg tgagtctctg cccgtggaaa aagccgctac
    30601 cactcgccag ccggagcaca gcaatccgct ggagtgcgac acgctgtgtt ttatgggcac
    30661 caccgtggtg agcggcacgg cacaagcaat ggtgattgct acaggtgcca atacctggtt
    30721 tggtcaactg gcggggcgtg ttagtgagca ggaaagcgag ccgaatgcct ttcagcaagg
    30781 gatcagccgc gtcagtatgc tgctgattcg ctttatgctg gtgatggcac cggtggtgct
    30841 gttaatcaat ggctacacca aaggcgactg gtgggaagcg gcgctgtttg cgctttcggt
    30901 cgcggtaggc ctaacgccgg aaatgttgcc gatgattgtt acctcgacgc tggcgcgcgg
    30961 cgcagtaaag ctgtcgaaac agaaagtgat cgtcaaacat ctggatgcta ttcagaactt
    31021 tggcgcaatg gatattctgt gcactgataa aaccggcacc ctgacacagg ataaaattgt
    31081 gctggagaat cataccgata tctccggtaa aaccagcgaa cgcgtgctgc atagcgcgtg
    31141 gttgaatagt cattatcaga ccggacttaa aaacctgctc gatacagcgg tgctcgaagg
    31201 tacggatgaa gagtcagcgc gctcgctggc cagtcgttgg caaaaaatcg atgagattcc
    31261 gtttgatttc gagcgtcgcc ggatgtcggt ggtagtggca gaaaataccg agcaccatca
    31321 gctggtttgc aaaggtgcat tgcaggaaat cctcaatgtg tgttcgcagg tgcgtcacaa
    31381 tggcgagatt gtgccgctcg atgacatcat gctgcgtaag attaagcggg ttactgatac
    31441 gctgaatcgt caggggctgc gcgtggttgc ggtggcgacg aaatacctgc cagcgcgtga
    31501 aggagattac cagcgggcgg atgaatccga cctgatcctc gaaggatata ttgctttcct
    31561 tgatccgcca aaagagacaa ccgctccggc actgaaggca ttaaaagcga gtggaattac
    31621 cgtaaaaatc cttactggcg acagtgagtt agttgcagcg aaagtgtgcc atgaagtggg
    31681 gctggacgcg ggagaggtgg tcattggtag tgatattgaa acgctatctg acgacgaact
    31741 ggcaaatctt gcacagcgca ctacgctgtt tgcccgcctg acgccgatgc ataaagaacg
    31801 cattgtgacc ttactgaagc gcgaagggca tgtggttggc tttatgggcg atggtattaa
    31861 tgatgcgccc gccttacgcg ctgcggatat cggcatttct gtggacggcg cggtagatat
    31921 tgcccgtgaa gcggctgata tcatcctgct ggaaaaaagc ctgatggtgc tggaagaggg
    31981 ggttattgag ggacgtcgca ctttcgccaa catgctgaaa tacatcaaaa tgacggcgag
    32041 ttctaacttc ggtaatgtgt tcagcgtgct ggtggcgagt gctttcttgc ccttcctgcc
    32101 gatgttgccg ttacacttgc ttattcagaa cctgctgtac gatgtgtcac aggtggcgat
    32161 cccgtttgat aacgtcgacg acgagcaaat tcaaaagccg cagcgttgga atccggcgga
    32221 tctggggcgc tttatgatct tcttcggacc gatcagctcg atcttcgata ttctgacgtt
    32281 ttgcctgatg tggtgggtat tccatgccaa cacgccggaa acgcaaacgc tgttccagtc
    32341 gggatggttt gtggtgggct tactgtcgca aacgctgatt gtgcatatga tccgcacccg
    32401 ccgtgtgccg tttattcaga gctgtgcatc gtggccgtta atgatcatga ccgtgatcgt
    32461 gatgattgtc gggatcgcat tgccgttttc accgctggcc agttatctgc aattacaggc
    32521 gctgccgtta agctatttcc cgtggctggt tgcgattctg gcagggtata tgacattaac
    32581 ccagttggtg aaagggttct atagccgtcg ttacggctgg caataaagaa taaactgggc
    32641 acgatagccc cgattcagat tgcagacaaa gtaagctttg ttcatgccgg atgcggcgta
    32701 aatgccttat ccggccaaca aattcaataa attgcaggaa tcacgtaggc ctgataagcg
    32761 tagcgcatca ggcgattttg cgtttgtcat cagacttaat ccgggcatga tagcccggat
    32821 ttccatcaag attagcgacg aacagcgatc gcttcgatct caatcttcac gtctttcggc
    32881 agacgggcaa cttcaacgca agaacgtgcc gggaaggtgg cgttgtgttc ggtgaagaag
    32941 gcttcgtaag tggcgtttac ggttgcgaag tcgttcagat cttttacaaa cacggtagtt
    33001 ttaacgatgt cgcccacttt cagaccagcg gcttcgacga tcgcttttac gttatccagc
    33061 gactgacgtg cctgtgcagc gacgtctgcc ggtacttcgc ccgttttcgg atttaccggg
    33121 atctgaccgg aagtgatgat catattgccc agatcaacgc cctgtacgta aggaccgata
    33181 gctgccggtg cattttccgt cgcgatagtt ttgctcatga tttctccttt attacagcgg
    33241 taaaaagtct ggctcattat agggagccag acttttatta ccaaccgcaa ttaattggcc
    33301 agcaccacat tatgggaaaa ctctttttca cagtatttgc atttgagcgc gatatcattg
    33361 gcgcgttttc gcacggcaaa gctggaagaa accggttcgg catggctgat acagttgctg
    33421 ttcgggcaga ccagcacatt gtcgatgcgc tccggcagac ttgggcgcga tttacccacc
    33481 acttcatagt tgtcgatacg gttaaccgtg gcttgcggcg catacaatgc cagttgatct
    33541 acctggtctt cactcaaaaa ggtattttcg attttgatca gatctttgcg gcccatctcg
    33601 ccagaaggca ggttcagacc gatggtgatg cgctgatccg tttcggtcag cttgaacaga
    33661 ctcaacagct taaaaccgat ctgggcgggg atatggtcaa ttaccgtgcc gcgtttaata
    33721 gcttcaacct gcaatttatt atcgtgtgtc atctctattt ccccttacag taccagatcg
    33781 cgattcagaa ccagtgccag taacgcctgg cgagcgaaaa tcccgttgcc tgcctgctgg
    33841 aagtaccagg cgtgtggcgt tttatcaaca tccgtcgcaa tctcatcaac acgcggcagc
    33901 ggatgaagta ctttcatatt ggctttggcg ttgtggagat cgctggcgcg aagaacaaac
    33961 tgcgctttca cgttggcgta ctcggacggg tccagacgct ctttttgcac gcgggtcatg
    34021 tacaggatgt caacttccgc catcacttct tcaatagagc tgtgcagact ccatgcgatc
    34081 cctttttcat caagcatatc cagaatgtat tgcggcattg ccagcgcgtc cggcgcgatg
    34141 aagtaaaaac ggttgccgtc gaacttcgct aacgcctgag tcagggagtg aacggtgcgg
    34201 ccatatttca ggtcaccaac cattgcgacg tggagattgt ccagacgccc ctgggtttcc
    34261 tgaatggtga ataagtccag caaggtttgc gtcggatgtt ggttggagcc atcaccggca
    34321 ttcagtaccg gtacattgcc ggaaaactcg gtggccaggc gcgccgcacc ttcctgcgga
    34381 tgacgcatca ctatcgcatc gacgtaagtg ctgataaccg aaatggtatc ggccagcgtt
    34441 tcgccctttt tacccagtga tgtattggcg ctgtcggaga agcccaccac gctggcaccc
    34501 aggcggtgca tggaagtttc gaaagagagg cgggtacggg tagaggcttc gaagaaacag
    34561 ctggcaatga ctttgtgctt caacagctct ggttgcgggt ttgctttcag tttcgccgct
    34621 gtcgccagca ccagattaag gtcatcgcga ctaaggtcgt ttatggaaat gatatgtttc
    34681 tgatatagcg gattagccat cttttatctc ctgacgcctg ggcaaaaaaa agcccctcga
    34741 ttgaggggct gggaatgggt gatcaacggg aagaaaaacg gcaggccagc gtcttttttc
    34801 agacgcggta agacaaaatg tcgaacacac tgaaccatac atcctcccgg caaattgtcc
    34861 ggcattatac tcatcgtcag aagcggtgca agcatttgat gcaatatttt gtcagcgcaa
    34921 acggtttatt tgaattataa gtcaaaatat atgtattttt atttactgtg attctggagg
    34981 ggaactgtcg ttcggtatca ataattgcag acatttcctg cttttttctt tttcactttc
    35041 acgcaatcag atatgcattt tattccattc atccgactta tagggcggag tgtgaaaacg
    35101 aacggctaac actattgctt acagctcagg gatgcgcgct atcactttaa tttcaaaatc
    35161 aaagcctgcc agccatgtaa cgcccaccgc cgtccaggtt ggataaggtg gcgcgctaaa
    35221 tatttcattt ttcaccgtca tgatgtcttc aaattgtttt tctggatcgg tatgaaagct
    35281 cgtaacatca atgatatcgt caaaagtgca tcccgcagct gccagggtcg catgcaaatt
    35341 atcaaatgcc agtctgactt gttgctgaaa atcgggttct ggtgttccgt cctctcgact
    35401 tcctacttgc ccggaaacaa acagcaaatc gccggaacga atagccgcag aataacgatg
    35461 ttctgcatac agtgaatgtc ggccagcagg gaaaacagcg gttctttcta ccatttggtt
    35521 atcctcaaga tttacgacat gaacagaaga tttatattta ccaggagccg cttttagcgg
    35581 acgacgtgag taaacaaaac ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag
    35641 tcggttatgc gccaaacgcg ccatcaatgg tatgcatcgc gccggtaaca aaactggctt
    35701 ctggccctgc taaccatgcg accataccag cgacctcttc cggttgccca tgtcttttga
    35761 tagccataaa actatgcaac atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat
    35821 caattggccc tggctggacg acgttgatgg tgatcccacg cggtccaaaa tcacgggcca
    35881 gcccgcgcgc catgccttgt agggcagatt tactggcggc ataagcagcc atgcctgcaa
    35941 caggcatacg atcgccattc acggagccga tgattaagat gcgcccacct tcgggcatct
    36001 gccgggcggc ttcaacagag gcatggtaag gagcatgaat attgattttg aaaaggcgat
    36061 caatatcgtc ggcatttaat tccagggcat cgccaaagac gccaatacct gcatttacca
    36121 ccaggatatc caatgcgccg ctcttacgaa cgacatcaat gacagcgtct ctgtcagcac
    36181 tatctgtaaa tactgctgtc gctcccgtct cttgtgccag gcgttcagcg gcatctttcg
    36241 accccgcata ggtgaatcgt acattggccc catcggtgac gaaacgacgt acgatagcgg
    36301 caccgattcc acgactgcca ccgaggatga gaactgtctt acctgtaaaa gcgcccataa
    36361 ggactccttg atttattatg tgacatgcat tacaaaactg ttttaacttt ctgtcaacaa
    36421 gttttgtaat ggtcactaaa aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc
    36481 ctgagcaggc agtctctgcg gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca
    36541 gtgttgctga agttactgat tatcttggta ttaacccccc gagcctctac gcggcttttg
    36601 gcagtaaggc tgggttattt agccgtgtac tcaatgaata tgtcggtacg gaagctattc
    36661 cgcttgtcga tattcttcgt gatgatcgtc cggtagggga gtgcctggct gaggtattaa
    36721 aagaagcggc gcgcagatat agccaaaacg gcggctgcgc tggctgtatg gttcttgaag
    36781 gcattcatag tcatgatcca caagcgcgtg atattgccgt tcaatattat cacgccgcag
    36841 aaacggccat ttatgactat atcgccaggc tgcatccaca aagcgcacaa tgtgtgactg
    36901 attttatgag taccgtgatg tcagggcttt ctgcgaaggc acgagagggg cactccctcg
    36961 aacaactctg tgcaactgct gcactggcgg gggaagcgat aaaaactatt ctcaaggagt
    37021 gatgctggcc ttgatccgaa aggcgggaac gcgcctgccg ataagttgtg ataagacaat
    37081 aattcacgcg ttaaggctgg ctgaattgat catcttttcg tataacggtg gaaatgaaac
    37141 attgttttaa ttaaggagtg gaaaagatga tcatcggaaa tattcataat cttcagccgt
    37201 ggctacccca ggagttacgc caggcgattg agcatatcaa agcacacgtt acggcagaaa
    37261 cgccaaaggg caagcacgat atcgaaggta atcgtctgtt ttatcttatc tcggaagata
    37321 tgaccgagcc gtacgaagct cgccgtgcgg agtaccatgc ccgctatctc gacattcaga
    37381 ttgtgttaaa aggtcaggaa ggcatgacct tcagcacaca accggcaggt acgccggata
    37441 ccgactggtt agctgataaa gacatcgcat ttttgccgga aggcgttgat gagaaaacag
    37501 ttatccttaa tgaaggtgat tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt
    37561 gcgcagtggg tgcaccagcc caggttcgca aagcagtagt gaagatgctg atggcgtgat
    37621 gacttttcgc cgtaaataac tccaggttta cggcgagttt gtgaaaagag cgttttttga
    37681 tatttttttg tgagtaaaat ttgtaatgct tagacgttct tattaactca aggagttcgt
    37741 catgagcaaa atatcagatt tgaattattc tcaacacatt acattagccg acaattttaa
    37801 acaaaaaagt gaagttttaa atacctggcg tgttggaatg aataattttg cccgtaatgc
    37861 cggggggcag gataacacaa gaaatatcct taatcctaag acatttttgg agtttttggt
    37921 aaaaatattt accctgggtt atgtggattt tagcaaacgc tccaacgaag cgggaagaaa
    37981 tatgatggct catattgagt cctcatctta tatcaaaaat aatgatggca gtgagataat
    38041 gaagtttgtt atgaataatc ctgaagggga acgagcggat tcacccaagg tgattataga
    38101 aatttcactt tccactatta ctactatggg gactcgtcaa ggacatacag ccattatatt
    38161 tccacaacct gatggttcga ctaaccgtta tgaaagaaag tcctttgaaa gaaaagatga
    38221 gagttcatta cacctgatta ctaacaaggt tctggcgtgt taccaacgcg aagctaacaa
    38281 ggaaatagct cgtctat
//
LOCUS       DAZFTN010000039        38066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFTN010000039 DAZFTN010000000
VERSION     DAZFTN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            116..727
                     /gene="ruvA"
                     /locus_tag="ACHKV2_002130"
     CDS             116..727
                     /gene="ruvA"
                     /locus_tag="ACHKV2_002130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO6940011.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            736..1746
                     /gene="ruvB"
                     /locus_tag="ACHKV2_002131"
     CDS             736..1746
                     /gene="ruvB"
                     /locus_tag="ACHKV2_002131"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO6940012.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1992..2777)
                     /gene="znuB"
                     /locus_tag="ACHKV2_002132"
     CDS             complement(1992..2777)
                     /gene="znuB"
                     /locus_tag="ACHKV2_002132"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO6940013.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2774..3529)
                     /gene="znuC"
                     /locus_tag="ACHKV2_002133"
     CDS             complement(2774..3529)
                     /gene="znuC"
                     /locus_tag="ACHKV2_002133"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO6940014.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3608..4540
                     /gene="znuA"
                     /locus_tag="ACHKV2_002134"
     CDS             3608..4540
                     /gene="znuA"
                     /locus_tag="ACHKV2_002134"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO6940015.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4556..5878
                     /gene="mepM"
                     /locus_tag="ACHKV2_002135"
     CDS             4556..5878
                     /gene="mepM"
                     /locus_tag="ACHKV2_002135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO6940016.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5998..6969
                     /gene="lpxM"
                     /locus_tag="ACHKV2_002136"
                     /gene_synonym="msbB"
     CDS             5998..6969
                     /gene="lpxM"
                     /locus_tag="ACHKV2_002136"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6940017.1"
                     /translation="METKKDNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7100..8542)
                     /gene="pyk"
                     /locus_tag="ACHKV2_002137"
     CDS             complement(7100..8542)
                     /gene="pyk"
                     /locus_tag="ACHKV2_002137"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO6940018.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8670..9539)
                     /gene="hexR"
                     /locus_tag="ACHKV2_002138"
                     /gene_synonym="yebK"
     CDS             complement(8670..9539)
                     /gene="hexR"
                     /locus_tag="ACHKV2_002138"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO6940019.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9877..11352
                     /gene="zwf"
                     /locus_tag="ACHKV2_002139"
     CDS             9877..11352
                     /gene="zwf"
                     /locus_tag="ACHKV2_002139"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO6940020.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11587..13398
                     /gene="edd"
                     /locus_tag="ACHKV2_002140"
     CDS             11587..13398
                     /gene="edd"
                     /locus_tag="ACHKV2_002140"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO6940021.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13435..14076
                     /gene="kdgA"
                     /locus_tag="ACHKV2_002141"
                     /gene_synonym="eda"
     CDS             13435..14076
                     /gene="kdgA"
                     /locus_tag="ACHKV2_002141"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6940022.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14132..15310)
                     /gene="purT"
                     /locus_tag="ACHKV2_002142"
     CDS             complement(14132..15310)
                     /gene="purT"
                     /locus_tag="ACHKV2_002142"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO6940023.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15444..15734
                     /gene="yebG"
                     /locus_tag="ACHKV2_002143"
     CDS             15444..15734
                     /gene="yebG"
                     /locus_tag="ACHKV2_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO6940024.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15801..16157
                     /gene="yebF"
                     /locus_tag="ACHKV2_002144"
     CDS             15801..16157
                     /gene="yebF"
                     /locus_tag="ACHKV2_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO6940025.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16484..17143
                     /gene="yebE"
                     /locus_tag="ACHKV2_002145"
     CDS             16484..17143
                     /gene="yebE"
                     /locus_tag="ACHKV2_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFO6940026.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17352..19412
                     /gene="ptrB"
                     /locus_tag="ACHKV2_002146"
     CDS             17352..19412
                     /gene="ptrB"
                     /locus_tag="ACHKV2_002146"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO6940027.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19409..20071)
                     /gene="exoX"
                     /locus_tag="ACHKV2_002147"
     CDS             complement(19409..20071)
                     /gene="exoX"
                     /locus_tag="ACHKV2_002147"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO6940028.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20095..20751)
                     /gene="yobB"
                     /locus_tag="ACHKV2_002148"
     CDS             complement(20095..20751)
                     /gene="yobB"
                     /locus_tag="ACHKV2_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO6940029.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20853..21083)
                     /gene="holE"
                     /locus_tag="ACHKV2_002149"
     CDS             complement(20853..21083)
                     /gene="holE"
                     /locus_tag="ACHKV2_002149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO6940030.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21222..21596
                     /gene="yobA"
                     /locus_tag="ACHKV2_002150"
     CDS             21222..21596
                     /gene="yobA"
                     /locus_tag="ACHKV2_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO6940031.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21600..22472
                     /gene="copD"
                     /locus_tag="ACHKV2_002151"
     CDS             21600..22472
                     /gene="copD"
                     /locus_tag="ACHKV2_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO6940032.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22485..22826
                     /gene="yebY"
                     /locus_tag="ACHKV2_002152"
     CDS             22485..22826
                     /gene="yebY"
                     /locus_tag="ACHKV2_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO6940033.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23222..23878
                     /gene="pphA"
                     /locus_tag="ACHKV2_002153"
     CDS             23222..23878
                     /gene="pphA"
                     /locus_tag="ACHKV2_002153"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6940034.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23879..24070)
                     /gene="yebW"
                     /locus_tag="ACHKV2_002154"
     CDS             complement(23879..24070)
                     /gene="yebW"
                     /locus_tag="ACHKV2_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO6940035.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24175..24411)
                     /gene="yebV"
                     /locus_tag="ACHKV2_002155"
     CDS             complement(24175..24411)
                     /gene="yebV"
                     /locus_tag="ACHKV2_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO6940036.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24529..25968)
                     /gene="rsmF"
                     /locus_tag="ACHKV2_002156"
     CDS             complement(24529..25968)
                     /gene="rsmF"
                     /locus_tag="ACHKV2_002156"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO6940037.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26049..28682)
                     /gene="yebT"
                     /locus_tag="ACHKV2_002157"
     CDS             complement(26049..28682)
                     /gene="yebT"
                     /locus_tag="ACHKV2_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO6940038.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28651..29934)
                     /gene="yebS"
                     /locus_tag="ACHKV2_002158"
     CDS             complement(28651..29934)
                     /gene="yebS"
                     /locus_tag="ACHKV2_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO6940039.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30064..30561
                     /gene="msrC"
                     /locus_tag="ACHKV2_002159"
     CDS             30064..30561
                     /gene="msrC"
                     /locus_tag="ACHKV2_002159"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO6940040.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30658..31356
                     /gene="proQ"
                     /locus_tag="ACHKV2_002160"
     CDS             30658..31356
                     /gene="proQ"
                     /locus_tag="ACHKV2_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO6940041.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31376..33424
                     /gene="prc"
                     /locus_tag="ACHKV2_002161"
     CDS             31376..33424
                     /gene="prc"
                     /locus_tag="ACHKV2_002161"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO6940042.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33616..34497
                     /gene="htpX"
                     /locus_tag="ACHKV2_002162"
     CDS             33616..34497
                     /gene="htpX"
                     /locus_tag="ACHKV2_002162"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO6940043.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSQDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34543..35916)
                     /gene="yebQ"
                     /locus_tag="ACHKV2_002163"
     CDS             complement(34543..35916)
                     /gene="yebQ"
                     /locus_tag="ACHKV2_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940044.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36093..36884
                     /gene="kdgR"
                     /locus_tag="ACHKV2_002164"
     CDS             36093..36884
                     /gene="kdgR"
                     /locus_tag="ACHKV2_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO6940045.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37028..37267)
                     /gene="yobH"
                     /locus_tag="ACHKV2_002165"
     CDS             complement(37028..37267)
                     /gene="yobH"
                     /locus_tag="ACHKV2_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO6940046.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37427..37570
                     /gene="mgrB"
                     /locus_tag="ACHKV2_002166"
     CDS             37427..37570
                     /gene="mgrB"
                     /locus_tag="ACHKV2_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO6940047.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37645..37932
                     /gene="yebO"
                     /locus_tag="ACHKV2_002167"
     CDS             37645..37932
                     /gene="yebO"
                     /locus_tag="ACHKV2_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO6940048.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9746 a         9373 c         9817 g         9130 t
ORIGIN      
        1 ccatttttca gttcatcgag acacctcgca agtttttttc atccttcgct ggatatctat
       61 ccagcatttt tttatcatac agcattatct ttgattcatt acgcaggagc gtcatgtgat
      121 aggcagactc agaggcatca tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg
      181 cggcgtaggc tatgaagtgc atatgccgat gacctgtttt tatgaactcc ctgaagcggg
      241 tcaggaagcg atcgttttca cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg
      301 ttttaacaat aaacaagagc gcacgttgtt caaagagttg atcaaaacca acggcgtcgg
      361 cccgaagttg gcgctggcga tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt
      421 tgagcgtgaa gaagtggggg cactggtgaa actgccgggt attggcaaaa aaaccgccga
      481 acgcttgatt gttgaaatga aagaccgatt taaaggtttg catggcgatc tctttacgcc
      541 agccgccgac ctggtactca cgtctcctgc cagcccggcg accgacgatg ctgaacaaga
      601 agcggttgcc gcgctggtgg cgctgggcta taaaccacaa gaagcaagcc gcatggtgag
      661 caaaatcgct cgccctgacg ccagcagtga aacattaatt cgcgaagccc tacgcgccgc
      721 gttatgaggt aaaggatgat tgaagcagac cgtctgattt ctgccggtac cactttgccg
      781 gaagatgtag cagatcgcgc cattcgccct aaattactgg aagagtatgt tggtcagccg
      841 caggttcgtt cacagatgga gattttcatc aaagcagcga aactgcgcgg cgatgccctc
      901 gatcatttgt tgatttttgg tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc
      961 gccaatgaaa tgggcgttaa tttacgcacg acttctggtc cggtgctgga aaaggcgggc
     1021 gatttggctg cgatgctcac taaccttgaa ccgcatgacg tgctgtttat tgatgagatc
     1081 caccgtttat cgccagttgt tgaagaagtg ctgtacccgg caatggaaga ctaccaactg
     1141 gatatcatga ttggtgaagg tccggcggca cgctccatta aaattgattt gccgccgttt
     1201 accctgattg gtgcaaccac gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt
     1261 ggtattgtgc aacgtctgga gttttatcag gtgccggatc tgcaatatat cgtcagtcgc
     1321 agcgcacgct ttatggggct tgagatgagt gatgacggcg cgctggaagt tgctcgtcgc
     1381 gctcgcggta cgccgcgcat tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa
     1441 gtgaagcacg atggcaccat ctcggcagat atcgctgctc aggcgctgga tatgttgaat
     1501 gtcgatgctg aaggtttcga ttatatggac cgcaaattgt tgctggcggt aatcgataag
     1561 ttctttggtg ggccggtagg tctggataac cttgcggcag ccattggcga agaacgtgaa
     1621 accattgagg atgtgctgga accttatttg attcagcaag gctttttgca gcgtacaccg
     1681 cgtgggcgta tggcgacgac gcgggcgtgg aatcactttg gcataacgcc gccagaaatg
     1741 ccgtaagtcg gattggtcgc tcgcaccgca tccgacattg gcagtgtgcc tgatgcgacg
     1801 ctattcgcgt cttatcaggc atataccaga gatagcgaac cgtaggtcgg aaaaggcgct
     1861 cgcgccgcat ccgacattgg cagtgtgcct gatgcgacgc tgttcgcgtc ttaccaggcc
     1921 taccagagat agcgaaccgt aggtcggata aggcgctcgc gccgcatccg acaaatgtgt
     1981 tcagcgatag attagctggc ctgctttttc atcatactga taataaataa cagtgccgca
     2041 catagcacca ccgacggacc cgccggcgta tcgtaaaacg cggaaaaggt taaaccgtca
     2101 gttactgcca ccatccccac caaaacagcg acaccagcca tctgttccgg cgtacgggca
     2161 aagcgacgcg cagtagctgc aggaataatc agcaacgaag taataatcaa cgcaccgaca
     2221 aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca tcaacaacaa tttcactcgc
     2281 tgtaatttca caccatcaac aaacgccaga tccgggctaa tcgtcatcga cagcaaattg
     2341 cgccattgcc agaacaaaat agccaccacg atgaccacgc caattgcaat agagatgaga
     2401 tcttctggcg tcactgccag taaatcaccg aacaggtaag ccatcaaatc aacacgaatg
     2461 ttagacatca gactaacgac caccaggccc agcgacaggg cactgtgcgc cataatccct
     2521 aataacgtgt cgatcgccag ctgtggacgc ttctccagcc ataccagacc gcccgccagc
     2581 agcagcgtaa cggcaatcac cgcatagaat ggattcacgt ccagcaacaa accaaacgcg
     2641 acaccaagta atgaggcatg agccagcgta tcaccgaaat aagacatacg acgccagact
     2701 acaaacgaac ccagcggacc cgcggcacag gcgagcatga tcccggctaa ccaaccggga
     2761 aataataatt caatcatgag cgatcatttc cccgacgcaa aacaattcgt ccctgtaaat
     2821 cgtgacgatg attatgatga tggcgataga tacccagttg ttcagcacca cgaggaccaa
     2881 acattgaaat aaactccgga tgcagggaaa caacttccgg tgtgccggaa caacaaatgt
     2941 gatgattcag gcacagcact tcatcggttt ttgccatcac cagatgaaga tcgtgagaaa
     3001 ccattaaaac gccacaatcc agttcacgac gcagttggtc aataaggtca tataacgcca
     3061 cctggccatt tacatccacg ccttgagtgg gttcatccag cactaataat tgcgggcgat
     3121 ttaacaatgc tcgcgctaac agtacacgct gcgtttcgcc accagagagc ttttgcatcg
     3181 gtgcgttaat caggtgcccg gcctggacac gtttcagtgc aggcaaaata tcttctttat
     3241 gtgtaccagg gcgtaagcgt aaaaaacggt ttacggtcag tggcaacgtg gtgtcgagat
     3301 acagcttctg cggtacatag ccgatgcgca gttttccgtt gcgcttgata accccttcat
     3361 cgggtgttac cagcccgagc actacccgta ccagtgtcga cttacctgcg ccatttggcc
     3421 caagtaaagt caaaattttt ccaggtttaa gttccagcga cacatcagag aggacgcggc
     3481 gttggccaaa agaaaccgag acattttcca gggaaaccag acttgtcatg ttaattttag
     3541 tcttgcagta gtcatgaaat gttataatat cacacttctc atattcatta cgatgattgg
     3601 tcgcattatg ttacataaaa aaacgcttct tttcgcagca ttattcgccg ctctctgggg
     3661 cggtgcaaca caggccgcag atgccgccgt tgtcgcttcg cttaaacccg ttgggttcat
     3721 cgcttctgcc attgctgatg gggtaacaga aacggaggtt ttacttcctg acggcgcttc
     3781 agaacatgat tattcactgc gtccatcgga tgtaaaacgc ttacagaacg cggacttagt
     3841 cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa ccggtaagca aattaccaga
     3901 agcgaagcag gtaacgattg cgcagcttga agatgtgaaa ccgctactga tgaaaagtat
     3961 taacggcgat gatgatgatc acgaccacgc ggaaaaaagt gacgaagatc accatcacgg
     4021 cgatttcaac atgcatcttt ggctttcccc agagatagcg cgggctacag cggttgcaat
     4081 ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc aaacttgacg ccaacctgaa
     4141 ggattttgag gcacaattag cctcaaccga aacgcaggtt ggtaacgagc tcgcgccgct
     4201 caaggggaaa ggttatttcg tttttcacga tgcttacggc tatttcgaaa aacagttcgg
     4261 actgacaccg cttggtcatt ttaccgttaa ccctgagatt caacctggcg cgcagcgttt
     4321 acatgaaata agaacacagt tggttgagca aaaagcaacc tgcgtttttg ctgagccaca
     4381 gttcaggcca gcggtcgttg agagcgtcgc acgagggaca tccgttcgta tgggaacgtt
     4441 ggatcccttg gggacgaata tcaaactggg taaaacaagc tattcagaat tcctgagtca
     4501 attagccaac cagtatgcga gctgcctgaa aggagattaa tgaggaagtg attacgtgca
     4561 acagatagcc cgctctgtcg ccctggcgtt taataattta ccgcgaccac accgcgttat
     4621 gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc tggcggccct atgtttatca
     4681 ccgcgatgcc acgccaattg tcaaaaccat tgagctggaa cagaacgaaa ttcgttcgct
     4741 cttacctgaa gccagtgagc cgattgatca agctgcacaa gaagatgaag ccattcccca
     4801 ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg catgaatatg ttgtttccac
     4861 tggcgatacg ctaagcagca ttctcaatca gtatggtatt gatatgggtg atatcaccca
     4921 actggctgcg gccgacaaag aattgcgtaa cctgaaaatc ggtcaacaac tctcctggac
     4981 attaaccgcg gacggcgaac tgcagcgcct cacctgggaa gtgtctcgtc gtgaaacccg
     5041 aacctatgac cgtactgccg ctaacggttt taaaatgacc agcgaaatgc agcaaggaga
     5101 gtgggttaac aatctgctga aaggtaccgt cggggggagc tttgttgcca gcgccagaaa
     5161 cgccggtttg accagcgccg aagtgagcgc agtgattaaa gccatgcagt ggcaaatgga
     5221 tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtatta atgtctcgag aaatgcttga
     5281 tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg cgttcagaag gtaaagatta
     5341 ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt aacggtactg gtctggcgaa
     5401 aggattcttg cgattcccga cggcgaaaca gttccgtatc tcgtctaact ttaacccgcg
     5461 tcgtactaat ccggtgaccg gtcgcgttgc accacacaga ggtgttgatt tcgccatgcc
     5521 gcagggtacg ccagtgcttt cagtgggtga cggtgaagtg gtggttgcca aacgcagtgg
     5581 cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc tacaccacgc gttatatgca
     5641 cttgcgcaag attctggtga aaccggggca gaaggtgaaa cgtggcgacc gtatcgcgct
     5701 ttccggtaat accggacgtt caaccgggcc gcatctgcac tatgaagtat ggataaacca
     5761 gcaggccgta aacccgctga cggcaaaact gccgcgtacc gaagggctga ccggctccga
     5821 tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg cagctacggt ttgattaatt
     5881 aacatccatt cgcagccggt acgcagtcag taccggcttt ttttatttgg tgcggggcaa
     5941 gttgcgccgc tacactatca ccagattgat ttttgcctta tccgaaactg gaaaagcatg
     6001 gaaacgaaaa aagataatag cgaatacatt cctgagtttg ataaatcctt tcgccacccg
     6061 cgctactggg gagcatggct gggcgtagca gcgatggcgg gtatcgcttt aacgccgcca
     6121 aagttccgtg atcccattct ggcacggctg ggacgtattg ccggacgact gggaaaaagc
     6181 tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc cagaacgtag tgaagctgaa
     6241 cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc aagcgatggc aatgatggct
     6301 gagttggcaa tacgcgggcc ggagaaaatt cagccgcgcg ttgactggca agggctggag
     6361 atcatcgaag agatgcggcg taataacgag aaagttatct ttctagtgcc gcacggttgg
     6421 gccgtcgata ttcctgccat gctgatggcc tcgcaagggc agaaaatggc agcgatgttc
     6481 cataatcagg gcaacccggt ttttgattat gtctggaaca cggtgcgtcg tcgctttggc
     6541 ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca tccagtcggt acgtcagggg
     6601 tactggggat attatttacc cgatcaggat catggcccag agcacagcga atttgtggat
     6661 ttctttgcca cctataaagc gacgttgccc gcgattggtc gtttgatgaa agtgtgccgt
     6721 gcgcgcgttg taccgctgtt tccgatttat gatggcaaga cgcatcgtct gacgattcag
     6781 gtgcgcccac cgatggatga tctgttagag gcggatgatc atacgattgc gcggcggatg
     6841 aatgaagaag tcgagatttt tgttggtccg cgaccagaac aatacacctg gatactaaaa
     6901 ttgctgaaaa ctcgcaaacc gggcgaaatc cagccgtata agcgcaaaga tctttatccc
     6961 atcaaataaa aaagcctctc gcgaggagag gccttcgcct gatgataagt tcaagtttgc
     7021 ttcagaatat tcgaaatctg ttgaactatc attgaactgt aggccggatg tggcgttttc
     7081 gccgcatccg gcaacgtact tactctaccg ttaaaatacg cgtggtatta gtagaaccca
     7141 cggtactcat cacgtcgccc tgggtgacaa tcaccaggtc accagacatc aagtaacctt
     7201 tatcgcgcag cagattaacc gcttcgctgg cagctgctac gccgtcatta gcgctatcaa
     7261 agtgcaccgg cgtaacgcca cgatagagag cagtcaggtt cagcgtacgt tcatggcgcg
     7321 acatggcgaa aattggtaga ccagagctga tacgggaggt catcagcgcg gtacgacccg
     7381 attcggtcat ggtaatgatc gccgtaacgc ctttcaggtg gttagctgcg tacattgctg
     7441 acatggcaat agcttcttcc acattgtcga actgaacgtc cagacggtgt ttagaaacgt
     7501 tgatgctcgg gattttttcc gcacccaggc aaacgcgcgc catggctgca acagtttctg
     7561 acggatactg cccagcggca gtttctgcag acagcatcac agcgtcagta ccatccagaa
     7621 cggcgtttgc tacgtccatg acttctgcac gcgtcggcat cgggttagta atcattgact
     7681 ccatcatctg ggtcgccgtg attaccgctc ggtttagctg acgcgcacga cggatcaacg
     7741 ctttctgaat gccgaccagt tccgggtcgc caatttccac accgaggtcg ccacgtgcaa
     7801 ccattaccac gtcagaggcg aggatgatgt catccattgc atcctggctg caaacggctt
     7861 ccgcacgttc aaccttggca acaattttcg catcacatcc tgcatcgcgt gccaggcgac
     7921 gggcatagtt cagatcttcg ccacagcgtg ggaaggagac agccaggtaa tctacgccaa
     7981 tcaacgccgc agtcttaatg tctgctttgt ctttttcggt cagcgcttca gccgacaaac
     8041 cgccgccaag tttgttgata cctttattgt tggagagggg accaccgacg gtgacttcgg
     8101 tgaacacttt catgccctga acttccagta cttttaactg gacgcgacca tcgtccagca
     8161 gcaggatgtc accaggcacg acgtcagcag gcaggccttt gtagttgata ccgacttttt
     8221 ctttgtcgcc ttcaccttta cccaggttgg cgtcgagcag gaatttatcc ccaatattga
     8281 ggaaaacttt gccttcttta aaggtggata cacggatttt gggcccctgg aggtcaccca
     8341 gaatagccac atgacgcccc agttttgcgg caatctcacg aactttatcc gcgcgcattt
     8401 tgtgatcttc aggcgagccg tgagaaaagt tcatacgtac aacgttggca cccgccgcga
     8461 taactttttc aagattatta tcgcgatctg ttgctgggcc taacgtggta acgatttttg
     8521 ttctgcgaag ccttctggac atgtaatact ccgttgactg aaacaaccag gtgttgcttg
     8581 aacatgaaat ccgaatgaaa taacgccgcg atggcgataa aataatctca ttcggatgtc
     8641 gtataagatt agaacagtga cagtcgcttt tagcgatcgt cacttaaatt aagtaactgc
     8701 ttatcaaaac gcgattcctt cagcgcttcc ttgacccgct tcaagttatc tctgaatttt
     8761 gcaccgcgtc gcaaagtaaa tcctgtcgcc agcacatcta tcacggtcag ctgtgcaagt
     8821 cgagaaacca tgggcatata aatgtcagta tcttccggta cgtcgagggt aattgccagc
     8881 gttgcttccc gggcgagcgg ggtacccgca gaggtgaggg caatcaccat agcgtcgttt
     8941 tcgcgtgcca gctgcgccag ctcgaccaga ttttttgttc ttccggtgtg agaaatcagc
     9001 accaccacgt ctccgtcgct acaattcata caactcatgc gttgcagcac aatatcatcg
     9061 gagtacacca ccggaacatt aaaacgaaag aacttattca tcgcatcgtg ggcaacggcg
     9121 gctgaagagc ctaatccgaa aaaggcgatt ttttttgcct gagtgagcaa gtcgacggcg
     9181 cggttgatgg cagatttatc cagtgaatga cggacatgat cgagcgttgc cattgcggac
     9241 tcaaatattt tccctgtgta tgattcaacg ctgtcatctt cattgacgtt gcgattaaca
     9301 tagggagtgc cattcgccag actctgtgcc agatgaagtt taaaatcagg aaaaccgcgc
     9361 gtgtccatgc tgcgacagaa acgattcacc gtcggttcgc taacattggc ttccagtgcc
     9421 agagcagcaa tactcgaatg gatcgcgtta tcgggcgaag ccagaatgac ctcggcaact
     9481 ttgcgctctg atttgctcaa atgttccagc tgagactgga ttttttccag catattcatg
     9541 atgtaaagag actcacgggt aatgacgatt tccgcactga aagaaatcga aatgcagttt
     9601 tgtcagatat tacgcctgtg tgctgtgtta atgacaaaag cagataaaaa agttgttatt
     9661 ttttttcata acatgatcag tgtcagattt ttacccaatg gaaaacgatg atttttttat
     9721 cagttttgcc gcactttgcg cgcttttacc gtaatcgcac gggtggataa gcgtttacag
     9781 ttttcgcaag ctcgtaaaag cagtacagtg caccgtaaga aaattacaag tataccctgg
     9841 cttaagtacc gggttagtta acttaaggag aatgacatgg cggtaacgca aacagcccag
     9901 gtctgtgact tggtcatttt cggcgcgaaa ggcgaccttg cgcgtcgtaa attgctgcct
     9961 tccctgtatc aactggaaaa agccggtcag ctcaacccgg acacccggat tatcggcgta
    10021 gggcgtgctg actgggataa agcggcatat accaaagttg tccgcgaggc gctcgaaact
    10081 ttcatgaaag aaaccattga tgaaggttta tgggacaccc tgagcgcacg tctggatttt
    10141 tgtaatctcg atgtcaatga cactgctgca ttcagccgtc tcggcgcgat gctggatcaa
    10201 aaaaatcgta tcaccattaa ctactttgcc atgccgccca gcacttttgg cgcaatttgc
    10261 aaagggcttg gcgaggcaaa actgaatgct aaaccggcac gcgtagtcat ggagaaaccg
    10321 ctggggacgt cgctggcgac ctcgcaggaa atcaatgatc aggttggcga atacttcgag
    10381 gagtgccagg tttaccgtat cgaccactat cttggtaaag aaacggtgct gaacctgttg
    10441 gcgctgcgtt ttgctaactc cctgtttgtg aataactggg acaatcgcac cattgatcat
    10501 gttgagatta ccgtggcaga agaagtgggg atcgaagggc gctggggcta ttttgataaa
    10561 gccggtcaga tgcgcgacat gatccagaac cacctgctgc aaattctttg catgattgcg
    10621 atgtctccgc cgtctgacct gagcgcagac agcatccgcg atgaaaaagt gaaagtactg
    10681 aagtctctgc gccgcatcga ccgctccaac gtacgcgaaa aaaccgtacg cgggcaatat
    10741 actgcgggct tcgcccaggg caaaaaagtg ccgggatatc tggaagaaga gggcgcgaac
    10801 aagagcagca atacagaaac cttcgtggcg atccgcgtcg acattgataa ctggcgctgg
    10861 gccggtgtgc cattctacct gcgtactggt aaacgtctgc cgaccaaatg ttctgaagtc
    10921 gtggtctatt tcaaaacacc tgaactgaat ctgtttaaag agtcgtggca ggatctgccg
    10981 cagaataaac tgactatccg tctgcaacct gatgaaggcg tggatatcca ggtactgaat
    11041 aaagttcctg gccttgacca caaacataac ctgcaaatca ccaagctgga tctgagctat
    11101 tcagaaacct ttaatcagac gcatctggcg gatgcctatg aacgtttgct gctggaaacc
    11161 atgcgtggta ttcaggcact gtttgtacgt cgcgatgaag tggaagaagc ctggaaatgg
    11221 gtagactcca ttactgaggc gtgggcgatg gacaatgatg cgccgaaacc gtatcaggcc
    11281 ggaacctggg gacccgttgc ctcggtggcg atgattaccc gtgatggtcg ttcctggaat
    11341 gagtttgagt aatatctgcg cttatccttt atggttattt taccggtaac atgatcttgc
    11401 acagattgta gaacaatttt tacactttca ggcctcgtgc ggattcaccc acgaggcttt
    11461 ttttattaca ctgactgaaa cgtttttgcc ctatgagctc cggttacagg cgtttcactc
    11521 ataaatcctc tgaatgaaac gcgttgtgaa tcatcctgct ctgacaactc aatttcagga
    11581 gcctttatga atccacaatt gttacgcgta acaaatcgaa tcattgaacg ttcgcgcgag
    11641 actcgctctg cttatctcgc ccggatagaa caagcgaaaa cgtcgaccgt tcatcgttcg
    11701 cagttggcat gcggtaacct ggcacacggt ttcgctgcct gccagccaga agacaaagcc
    11761 tctttgaaaa gcatgttgcg taacaatatc gccatcatca cctcctataa cgacatgctc
    11821 tccgctcacc agccttatga acactatcca gaaatcattc gtaaagccct gcatgaagcg
    11881 aatgcggttg gtcaggttgc gggcggtgtt ccggcgatgt gtgatggtgt cacccagggg
    11941 caggatggaa tggaattgtc gctgctaagc cgcgaagtga tagcgatgtc tgcggctgtc
    12001 gggctgtccc ataacatgtt tgatggtgct ctgttcctcg gggtgtgcga caagattgtc
    12061 ccgggtctga cgatggcagc cctgtcgttt ggtcatttgc ctgcggtgtt tgtgccgtct
    12121 ggaccgatgg caagcggttt gccaaataaa gaaaaagtgc gtattcgcca gctttatgcc
    12181 gaaggtaaag tggaccgcat ggccttactg gagtcagaag ccgcgtctta ccatgcgccg
    12241 ggaacatgta ctttctacgg tactgccaac accaaccaga tggtggtgga gtttatgggg
    12301 atgcagttgc caggctcttc ttttgttcat ccggattctc cgctgcgcga tgctttgacc
    12361 gctgccgctg cgcgtcaggt tacacgcatg accggtaatg gtaatgaatg gatgccgatc
    12421 ggtaagatga tcgatgagaa agtggtggtg aacggtatcg ttgcactgct ggcgaccggt
    12481 ggttccacta accacaccat gcacctggtg gcgatggcgc gcgcggccgg tattcagatt
    12541 aactgggatg acttctctga cctttctgat gttgtaccgc tgatggcacg tctctacccg
    12601 aacggtccgg ccgatattaa ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt
    12661 gaactgctca aagcaggcct gctgcatgaa gatgtcaata cggtggcagg ctttggtctg
    12721 tctcgttata cccttgaacc atggctgaat aatggtgaac tggactggcg ggaaggggcg
    12781 gaaaaatcac tcgacagcaa tgtgatcgct tccttcgaac aacctttctc tcatcatggt
    12841 gggacaaaag tgttaagcgg taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg
    12901 gttgagaacc aggtgattga agcgccagcg gttgtttttg aaagccagca tgacgttatg
    12961 ccggcctttg aagcgggttt gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg
    13021 ccaaaagcga acggaatgcc agaattacat aaactcatgc cgccacttgg tgtattattg
    13081 gaccggtgtt tcaaaattgc gttagttacc gatggacgac tctccggcgc ttcaggtaaa
    13141 gtgccgtcag ctatccacgt aacaccagaa gcttacgatg gcgggctgct ggcaaaagtg
    13201 cgcgacgggg acatcattcg tgtgaatgga cagacaggcg aactgacgct gctggtagac
    13261 gaagcggaat tggctgctcg cgaaccacac attcctgacc tgagcgcgtc acgcgtggga
    13321 acaggacgtg aattattcag cgccttgcgt gaaaaactgt ccggtgccga acagggcgca
    13381 acctgtatca ctttttaaga cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa
    13441 aactggaaaa caagtgcaga atcaatcctg accaccggcc cggttgtacc ggttatcgtg
    13501 gtaaaaaaac tggaacacgc ggtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc
    13561 gttctggaag tgactctgcg taccgagtgt gcagttgacg ctatccgtgc tatcgccaaa
    13621 gaagtgcctg aagcgattgt gggtgccggt acggtgctga atccacagca gctggcagaa
    13681 gtcactgaag cgggtgcaca gttcgcaatt agcccgggtc tgaccgagcc gctgctgaaa
    13741 gctgctaccg aagggactat tccgctgatt ccggggatca gcactgtttc cgaactgatg
    13801 ctgggtatgg actacggttt gaaagagttc aaattcttcc cggctgaagc taacggcggc
    13861 gtgaaagccc tgcaggcgat cgcgggtccg ttctcccagg tccgtttctg cccgacgggt
    13921 ggtatttctc cggctaacta ccgtgactac ctggcgctga aaagcgtgct gtgcatcggt
    13981 ggttcctggc tggttccggc agatgcgctg gaagcgggcg attacgaccg cattactaag
    14041 ctggcgcgtg aagctgtaga aggcgctaag ctgtaaaagt caaaatgccc gatcgaggat
    14101 cgggcatttt tgtagcgttt atttgccggg tttaaccctg tacttttacc tgtccggcgg
    14161 cgtgcttcgc gcgttcaatg gcgtcaacaa cactctctgc agtagccagt gccaccccca
    14221 gacgacggct gccatcaatt tccggcttac caaataaacg aatctgcaaa tctgcgccta
    14281 cggcattctg cacattatca aacgtgacat tctgactggt cagttgtggc agaataacgg
    14341 cagaagctgc aggaccatac tgacggatcc cgccaaccgg aagtccgagg aaggcacgta
    14401 catgcagggc aaactctgag agatcttgag aaattaacgt caccatcccg gtatcatgtg
    14461 gacgagggga gacctcactg aaaatcacct catcaccaca gacaaacagc tcgacaccaa
    14521 acagaccata accgcccagt gccagcacca ctttacgggc aatctcctgc gcacgttcaa
    14581 gggcaagcgg gctcatttgc tgtggttgcc aggattcaca gtagtcgcca tcttcctggc
    14641 gatgacctac tggtgcacag aaatggacgc catccaccgc gctgacggtt agcagggtaa
    14701 tttcgaagtc aaacttaacg acgccttcaa caattacgcg gcccgctccg gcgcgaccgc
    14761 cttgctgagc gtattcccat gcctgagcaa gttgctctgc agagcgaata aacgtctgcc
    14821 ccttgccgga agagctcatc accggtttta caatgcaggg atagccaatg gcggcaaccg
    14881 cctcgcggaa aaggctttcg ctatcggcaa aacgataagt ggaagtgggc agctgcagct
    14941 cttctgccgc caggcgacgg ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc
    15001 aggggacaac attcagtcct tcctcttcaa gttggatcag catatcggtg gcaatagctt
    15061 cgatctccgg cacgatataa tgtggttttt ccagttcaac cacacggcgt aatgcatcac
    15121 catcaagcat attaatgaca tgggagcgat gcgcgacatg catggctggt gcgtcggcat
    15181 agcgatcgac ggcaatcacc tctacgccga gacgctgaca ctcgattgcc acttctttac
    15241 ccagttcacc ggagcctaat aacatcacgc gagttgctgc cggacgcagc gcagtgccta
    15301 ataacgtcat aacgaatatc ccctgattaa ttccgcgcgc agtataaacg aaaacgtttg
    15361 cgtgtgtctt tattgctggt gttgatctct caaccgaaaa gaaatatact gtatagaatc
    15421 acagttatta tgagaggctg caaatggcgg ttgaagttaa atacgtagtc attcgtgagg
    15481 gcgaagagaa aatgtcgttt accagcaaaa aggaagccga tgcttatgac aaaatgcttg
    15541 atacggcgga tctgcttgac acctggctga caaattctcc agtgcaaatg gaagacgagc
    15601 aacgtgaagc cctttcgcta tggctggcag aacaaaaaga tgtgctgagc accattctga
    15661 aaaccggcaa attaccgtcc ccacaggtcg tcggtgctga atccgaagag gaagatgcct
    15721 cacacgcggc ctgaagcaac ttgcgctcct gacaatttgt accatgcttt ctcctgaatc
    15781 attgtgctat ggagaaaaac atgaaaaaaa gaggggcgtt tttagggctg ttgttggttt
    15841 ctgcctgcgc atcagttttc gctgccaata atgaaaccag caagtcggtc actttcccaa
    15901 agtgtgaagg tctggatgct gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa
    15961 atcgcgtggc gcgctgggca gatgatcaaa aaattgtcgg tcaggccgat cccgtggcat
    16021 gggtcagttt gcaggacatt cagggtaaag atgataaatg gtcagtaccg ctaaccgtac
    16081 gtggtaaaag tgccgatatt cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg
    16141 aatatcagcg gcgttaatgg gttctgataa ttcgattgac cgttggcgga tgcggcgcga
    16201 gtgccttgtc cgccccagaa cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg
    16261 catcgggcaa tcagcatcaa acaatgccac tcaaaacgac aaactacttt cacctaaaat
    16321 acccttatca cctgatatac agtaaatacc ttacctggcg cgaatttctt tgaataacgg
    16381 cctaccgccc cgcttatttg cgcatctttt gtctcgatat acattttctg acatcctcat
    16441 cctctatgct acaggcacta aaaatggtta ttgaggagca aatatggcta actggttaaa
    16501 tcaactgcaa tccctgcttg ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg
    16561 attgggcaaa cttttagtgc cgggcgcatt aggcggactg gctggactgc tggtcgcaaa
    16621 taaatcagca cgtaaacttc ttacgaaata tggcaccaac gcgttgctga ttggcggcgg
    16681 agcggtagcg ggtacggtgc tgtggaataa atacaaagat aaaattcgcg cggcgcatca
    16741 ggacgaaccg cagtttggct cgcaaagtac gccgctggat gagcgtacgg aacgtttgat
    16801 ccttgcgcta gtctttgccg ctaaaagtga tggtcatatt gatgccaaag aacgtgcggc
    16861 aatcgaccag caattgcgtg aagccggcgt ggaagagcag gggcgggtac tcattgagca
    16921 ggcaatcgaa caaccgctgg atccacaacg cctggctacc ggggtccgca atgaagaaga
    16981 ggcgctggaa atctatttcc tgagctgcgc ggctattgat attgaccatt ttatggaacg
    17041 cagttacctg aatgcactgg gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat
    17101 tgaacgcgat ctcgaacagc aaaaacgcac tttggcggaa taattccgct ctctggatag
    17161 cctgaagttg actatttcgg gctatccaga acagcacagc catttgaaca ggctgatttt
    17221 ctctgtctgc ataacattcg atattcgcgc cattacctgt catcatctaa gcaatgactg
    17281 ccctgtttcg cttgcatccc cggtgagttt tgccaccctt ataagatgtt tcaaccagaa
    17341 agaacaataa catgctacca aaagccgccc gcattcccca cgccatgacg cttcatggcg
    17401 atacgcgcat cgataattac tactggctgc gggacgatac gcgttctcag ccggaagtcc
    17461 tggactacct gcaacaagaa aatagttacg gtcatcgggt gatggcctca caacaagcct
    17521 tgcaggatcg catcttaaag gaaatcatcg accgcattcc gcaacgagaa gtttctgcgc
    17581 cctacatcaa aaatggctac cgctaccggc atatttatga accaggctgt gaatatgcta
    17641 tctaccagcg tcaatcggcg ttcagtgaag agtgggacga gtgggaaata ttgctcgatg
    17701 ccaacaagcg cgcggctcat agtgagtttt attcgatggg cggaatggcg attacgcccg
    17761 ataacaccat tatggcgctg gcagaagatt ttctttcccg acgccagtac ggcattcgtt
    17821 ttcgtaatct ggaaacaggt aactggtacc cggaactgct ggataacgtt gaacccagct
    17881 ttgtctgggc aaatgactcc tggactttct actatgttcg caagcatccg gtgacgctgc
    17941 tgccttatca ggtctggcgt cacgccatcg gtacgccagc atcgcaagat aaactgattt
    18001 acgaagaaaa agacgatacc tattacgtca gcctgcataa aacgacctca aagcactatg
    18061 tagtcattca tttggccagc gccaccacca gtgaagttcg cctgctggac gcggaaatgg
    18121 cagatgccga gccgtttgtt tttctgccgc gccgcaaaga tcacgaatac agccttgatc
    18181 actaccagca tcgtttttat ctgcgttcca accgccacgg caaaaacttt ggcttatacc
    18241 gtacccgtat gcgtgatgag caacagtggg aagagttaat tccgccacgc gaaaacatca
    18301 tgctggaagg gtttacgctg tttaccgact ggctggtggt tgaagagcgt cagcgcgggt
    18361 taaccagttt gcgccaaatt aaccgcaaga cccgggaagt cattggtatt gcctttgatg
    18421 atccagccta tgtgacctgg attgcctaca atccagaacc tgaaaccgcg cgattgcgtt
    18481 atggttattc ttccatgacc acaccagaca ctttgtttga actggatatg gataccggtg
    18541 agcgtcgtgt attaaaacaa acagaagttc ctggttttga tgcggcgaat taccgcagtg
    18601 aacacctgtg gatagtcgcc cgtgatggtg tcgaagttcc ggtttcgttg gtctaccatc
    18661 gcaatcattt tcgcaaagga cacaacccgc tgctggtgta tggctacggt tcttacggcg
    18721 caagtattga tgccgatttc agttttagcc gcttgagttt gttagatcgt ggctttgtct
    18781 acgccattgt ccatgttcgc ggcggcggtg agctggggca acaatggtac gaagacggta
    18841 aatttctgaa gaagaaaaat acgtttaatg attatcttga tgcctgcgat gcattgttaa
    18901 aactgggcta tggctctcct tcgctttgtt atgcgatggg cgggagtgcg gggggcatgt
    18961 tgatgggcgt tgcaattaat caacgcccgg aattattcca cggcgttatc gcccaggtac
    19021 cgtttgttga tgttgtaaca acgatgcttg atgaatcaat tcctcttacc actggtgagt
    19081 ttgaagagtg ggggaatccg caggatctgc aatattacga gtatatgaaa agctacagcc
    19141 cgtatgacaa cgtcaccgca caggcttatc cacatttact ggtaacgacc ggtttacacg
    19201 attctcaggt gcaatattgg gaaccggcaa aatgggtcgc taagttgcgc gagctgaaaa
    19261 ccgataacca tcttttattg ctctgtaccg acatggactc aggccatggc ggtaaatctg
    19321 gtcgctttaa atcgtacgaa ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc
    19381 agggaacatt acccgctagg cctgcggatt aggtattttc cagataatgt ttcagtgtta
    19441 aacgcagctc cgggctcatg ctgtccaggt tattaaacaa ccagcgcaga tagcccggat
    19501 cgcgttcggc aacgtcggaa accgctttgc cacggtattt gccaaaggtg aaggtcgtca
    19561 tcaacgacgg acgtccggtt atatcggcca tctgttctgc cgtccagccg gaggtgttca
    19621 taatatcgat aagcaacgcg gcggtgatat aacagtcata caacgcgcgg tgatgatgca
    19681 ggcccggcgg cgtctgtaca ttgagcttcc gtgttttata taacgccata ttgctgtact
    19741 tgatccctgg ccacaaacga cgggccagtt tcatagtgca aatccactca ccgggcatct
    19801 caggcagtac gcggcggtca aagctggcgt tatgcgcgac ataccattca ctaccgtaat
    19861 agtgtgggat cacatcttca atccacggtt tatcggcgac catggcttcg gtgatgcgat
    19921 gaatcgccat cgcttgtgga ctaataggac gatcggggtg caccaggtgg ctcatggggt
    19981 tgacgatttt cccgtcaatg acatcaacag aggcaatctc aacgatccct ccctgcaaac
    20041 cgcaggtttc tgtatcgata atgcgcaaca tggcatgctc caggccgaaa aagcctagcg
    20101 taatggaatg atatcgcctt gccaaccctg tgatgaacgc tgaccgacta acaataatga
    20161 accacgatcg gcgcgaacga tgagacgacc atgttcatcc catagtgcac tgttaccacg
    20221 ggcatttgcc atcagcacag caatcgagta ctgatgtgaa aaaaattgta agcgacgggc
    20281 cgatgccagc aggtcgggtt ctcccagaca ttgccctgtc gtaaacagtg aacaggtcgg
    20341 gtccatatcc ataccttgcg gttgttcatc gaccaccgtg atggttctgg aacgacggcc
    20401 cagacatgca ccatgactct gatggtagat cccaggcgtt tttcgccacg gtgcgaacac
    20461 cgcaattcca cgaataaagc gatcgttata ttcaacggga aggccggcaa tgatggtcat
    20521 tcgccaggtc gttgcggcat aacaaagcgg gtctaacagt gagagatcgg gtggggcagg
    20581 aagggcacgt cgcgaataat cacaccccag taaagaaagc gatggaaaaa ctaacagctg
    20641 acattgttgc ctggcggcgg ccctcacaaa ctccagatga tgagctacct gttcggtgag
    20701 tgaggtttta cgaggctcat attgcgctgc tgcaactttc cagaacgaca taatgacttc
    20761 cttttcaaaa tgactgcaaa ttatgggtgc ggtttagaaa atactcataa tttagcaggc
    20821 gttatgtaag aaagtgtaac tctgtataag ttttatttaa gtttgggctc gtaaggtaaa
    20881 cgtgacagat tgaccgaagc caaacggtgg gcaataagcc gctcgcgaaa ccagctgcgc
    20941 aaatgttcag gctgttcacg ttcaaccgct tcagcgatca ctggcatatt gtagcgttct
    21001 ttaaatgcca ccccggccgc cgccaaatcg acattcactt tatccatttc tgtttgatcc
    21061 agtttagcca gattcttcag cattcttaat ctcctcatca ttcgcgggca aaagttacta
    21121 agagtgagcc tggatggcaa gccggaattg atgatcctcg actcagacat tagacaaggt
    21181 tatgctctgt aaaaaagtta ttacaaaaag gaatagataa tatggcttca actgcacgct
    21241 cccttcgcta cgcgctggca atcctgacca cttcgttggt gacaccttcc gtctgggcac
    21301 atgcgcattt aacgcatcag tatcctgcgg caaacgcgca agtgacagct gcaccgcagg
    21361 caatcacttt aaacttctcg gaaggtgttg aaacaggatt cagtggtgca aaaatcacgg
    21421 ggccaaaaaa cgaaaatatt aaaacattac cggcgaagcg aaatgagcag gaccaaaagc
    21481 gactgattgt tccactggcc gattcgctga aacccggaac ctacaccgtt gactggcatg
    21541 ttgtttcggt ggacgggcat aaaacgaaag gacactacac ctttagcgtg aaataaatca
    21601 tgctggcgtt tacctggatc gcgctgcgat ttatccattt cacctcgctg atgctggtct
    21661 ttggcttcgc aatgtacggt gcctggctgg cacccttaac gattcgtcgc ttgctgacga
    21721 agcgttttct gcgtttacaa cagcacgctg ccgtctggag tttgatcagt gccactgcaa
    21781 tgctcgccgt tcagggcggg ttgatgggaa cgggatggaa agatgtattt tcgccaaaca
    21841 tttggcaggc tgttttgcaa acgcagtttg gtggcgtctg gttatggcaa attgttctcg
    21901 ccctcgtaac gttgatcgtc gcccttatgc aaccgcggaa tatgccacgt ttgctgttta
    21961 tgctcactac cgcgcaattt attctgctgg cgggagtggg gcatgcgacg ctgaatgaag
    22021 gggtaacggc gaaaatccat cagactaatc acgcgattca cctgatttgt gcggcggcgt
    22081 ggtttggggg attactgccg gtgctctggt gtatgcaact catcaaaggc cgttggcgac
    22141 accaggctat tcaggcgctg atgcgttttt cctggtgcgg gcattttgcg gtgatcggcg
    22201 tactggcaag cggcgtgctt aatgcattgt tgattaccgg atttcccccc acgcttacga
    22261 cttactgggg ccaactctta ttgctcaaag ccatcctggt gatgattatg gtggtcattg
    22321 cgctggctaa tcgttatgtt cttgtaccgc gcatgaggca ggatgaagat cgtgcggcac
    22381 cctggtttgt gtggatgact aaactcgaat gggcgatagg tgcggtagtg ctgctgatca
    22441 tcagcctgct tgcgactctc gaaccttttt gatggattac gaaaatgatg aaaaaaagta
    22501 ttctggcgtt tctgttactc accagttctg cagcggcgct ggctgcaccg caggtgatta
    22561 ccgtcagccg ttttgaagtg ggtaaagaca aatgggcgtt taatcgcgaa gaggtgatgc
    22621 tgacttgccg accgggtaat gctttgtatg tcatcaaccc aagtaccctc gtgcagtatc
    22681 ctttaaacga tgtcgcacag aaggaagttg ccagtgggaa gactaaagcc caacccattt
    22741 cggtgattca gattgatgat cccaacaatc ccggcgaaaa aatgagtctg gcaccgttta
    22801 tagaacgagc tgaaaaactc tgttaattac ctaaaatagc cttttgattt ccaataaaaa
    22861 aaccgcctca gttctttcac cagaacgggc ggtttttaac atttaagctg atgaccaccg
    22921 cgctttttat tgaccatttt gcacgcaaac tggaaaacct ggcgtcgtca tctattctta
    22981 aagggcaagg caactaagcc tgcattaatg ccaactttta gcgcacggct ctctcccaag
    23041 agccatttcc ctggaccgaa tacaggaatc gtgttcggtc tctttttatc tgttaaaagc
    23101 cagaagcatt tccttcgctg actttatagt caaccataac acacactcta ctgtctgagt
    23161 ccagcgtttt ttaacattct tgttaagatt atgtgatctt tagcgcggga ggaaaatatt
    23221 gatgaaacag cctgcgcccg tttatcagag aattgcgggt catcaatggc gacatatctg
    23281 gctttctggc gatatacacg gttgtcttga gcagttgcgc cgcaaattat ggcattgtcg
    23341 ttttgatccg tggcgagatt tacttatctc agtgggagac gttatcgatc gtgggccgca
    23401 aagtttacgt tgtctgcagt tactggaaca acattgggtt cgtgcggtaa gaggcaatca
    23461 tgaacagatg gcgatggatg cgctggcatc ccagcagatg tctttgtggt tgatgaatgg
    23521 cggcgactgg tttattgcgc tggcagataa tcaacagaaa caagcgaaaa cggcgctgga
    23581 aaaatgtcag catttgccct ttattcttga agtacacagc cgcaccggca aacatgttat
    23641 tgctcatgcc gattatccag atgatgttta tgaatggcaa aaggacgttg atttgcatca
    23701 ggtcttgtgg agccgctcgc gattaagtga acgccaaaaa gggcagggaa ttacaggtgc
    23761 tgatcatttc tggtttggtc atacaccgtt gcgccatcgc gtggatattg gcaacctgca
    23821 ttatattgat accggcgctg tctttggggg cgaactgact ttcgtgcaat tgcaataatt
    23881 aaaaatcacc atactcctgt gctggtcgcc agaaaccatc tataaaatcc tcaatcggaa
    23941 aacaaccgcc atgacggatc cgttgatcgc tcatagaata aagacactgc tgttccgtgt
    24001 tgtagacatc cacaacaata tcttcacaac cgccatccag gtagcaaaca aaaagtacca
    24061 gcgcgaacat ttcatccccg aagtgtggtg ccgtaccgtt aagtttagga gagattttac
    24121 aacgggggaa taaccaggac aaataacccg ccatgccgac gggttctttt tggatcaggc
    24181 aagacgcata atccaggcat cagattgttg atcgtaacgg gtacggtaaa ggacagagtg
    24241 ttcgccattt agcagggcag ggatactggt ttcagcatcc caggcatcca gttgcataca
    24301 taaatcgggg tctgatttac aaggaatggt taacgttcga tcacccggcg attcaccgtg
    24361 taattcgccg tcgtcgattt caaaagcgcc tatgcgcaca ctggttttca tcttcgtgct
    24421 ctccatttag ctgccggttg tggtacgacc agtccgatcg caccattttt cagcctagtt
    24481 gaggccgccg aatgtgccag tcaaaaagtg cgcttttttt gtgcgctgtc atgcgttact
    24541 ggtaaaaagt ttcccgtccc gcaccagttc acgcgggtag ctgtttttca gtcgcgaacc
    24601 aatccgtttg gctaaaccaa tcggctgatg ctggaaagta accaacacgt catccgccac
    24661 tggcgcggct tgcgggtaaa catcgcgccc gcgataccac tcctccgctt cttgcggtgt
    24721 cagttcaaaa gcgttctcgt tgtcggggga ggcaagggca ataaccgctt catgctgcca
    24781 gcgataacct ttgttgtgcg tttcggcaag tttaatcccc aggcgagaaa atcggacttt
    24841 accgatcagg gcttcaatgc ctaccggaaa caaccacact tctttgtcgc gctgccagag
    24901 tcgcaggttt ccatcccagt ttaagccaac acttgcagcc gcctgacgaa tttgtgcggc
    24961 ttcgcgatct ttcaccgggc tgaacgggaa attgcccact ttgtattttg gggtgggtaa
    25021 ggcgggaatg gcctgagttt tacgcagacg agcaacgaag aagccttcgc agtcgtaaat
    25081 ttgtgggaaa acatgcaaaa agccttcttc ggtcagcgct ttgtttgcac cagggaagag
    25141 atcgccaagt ggtaaaaact ctactgcgtc ggggtaagtc tctttaagcc acaggcaaac
    25201 ggattcgttt tcttcccggt ttaaggtaca ggtcgagtaa accagtgtac cgccagagcg
    25261 taatgcatga aaggcactgt cgatcagttc ccgttgggta gctgcgattt cctgattgct
    25321 ttctggtgac caatttttta gcgcatcggg atctttacgc accacgcctt cgccggagca
    25381 gggagcgtcc agcaaaatgg catcgaacat ttctggcaat gccgcaccaa acacgcggcc
    25441 atcaaaatgt gtgagcgcaa cattactgat accacagcgg ctgatattgg catgtaacac
    25501 ttttacccga ctggcggaaa actcattggc aaggattgcc ccttcgttat tcatccgcgc
    25561 ggcaatttgc gtcgttttgg agcctggcgc agcagcgaca tccatcaccc gctgtggtgc
    25621 attaccgtca gcaaacaagg cggcgacggg caacattgaa ctggcttcct gaatataaaa
    25681 caggccactt aaatgctcgg cggtactacc caatggcaat gcatcttcat cgtcgcgttc
    25741 aatccagaaa ccttcttcac accacggaat tggcgtaagc gtccagccat aaggagcggt
    25801 taatcgcagg aaatcagcaa cggaggtttt cagcgtatta acgcgaatgc tgcggcgcaa
    25861 cgggcgctga caggcggcaa gaaaatcatc aaaagagagc gtcgaaggca ttgcttcgcg
    25921 catttgcgtc agaaaggcgt ccgggaaata aacggtgtgt tgggccacga gcatatacca
    25981 ctggcaaaaa aaacaggcgc gcagtttagc ataaacgctc cggcgcaggc acgccggagc
    26041 agtgggcatt atttgggaag cgcagttccc cattcacgcc actctttcgg ttcactttcc
    26101 tgcaacagga agtgcttgcc ttcctgggct ttcggtgcca gtggcgtacc cggaggcgtg
    26161 gcgaaggcga tgccgccacg gataaactgg ttaaaggtgc cggttttcac tacgccgccc
    26221 gtcagaccaa agtccagact gtaacccgat gccaaccaga agacggaatt gttacgcacc
    26281 aggtgttgat agcgtttact gatgcgcatc gcaatcatca cgcgatctga caatgtcccc
    26341 agcgtcattc cggtaaccgt accgacttcc agaccacgga acagcacagg cgtaccgatg
    26401 cctaacgaac aggcttccgg cgcttcaaca ataatgctta aaccatccag gtaacgcgaa
    26461 tcagtaatgg tggcctcttg taattcaaag tcgcgacgag gattgccacg gcctggttcg
    26521 acgttgatat acggctggag gatagtatca agatgctcaa cgccagctgc cgaaatttgc
    26581 ggtgtgacca ctgagaagcg cgtaccaccg cgagcaaagg tctggacata ttccggatag
    26641 agcaccgcct ttgcctgtac ttcattgcgc gcggtaatca gatccagcgt ctggatttgc
    26701 ccgatatcaa taccgagata gcgaattggc atcccgaccg ccagttttcc ggcatcgaaa
    26761 gcatgaagcg taatctgccc gccaaccgca cgggccgctg tttcggaagc atacagaata
    26821 cgcttatcgc ctttacgctg actggcgctg gcaccgctga ggttatcgaa gctaatggct
    26881 ccctttaatg ctctggagag tggggatgcc tgtacggtca ggccactacc attcagctga
    26941 actttcgccc cgccttctgc ccagaacacg ctattgttgg tcagaaggtt gcgatactcc
    27001 ggcttaatat gcagatcgat atcaaacgcg ttagctcgcg gacgcacggt aataacttca
    27061 ccaacttcaa atttacggta cagcactacc gatcctgcct gcacatccgg cagcgtctct
    27121 gcactcaaac tcacggttgt ggtgggtaaa tcgctaaggc tgttctccag cgctttttcc
    27181 agattggcat acagtggata gctggctttc atctcacctt tatcgcccgg cagaatacgt
    27241 atcccgccat tgatccattc tgaggcgctg gcaccgagaa actcaacgcc atccagcccc
    27301 accttcacat cgacacggct gttgacgaca aatttgctat cgccttttac cagttcgcga
    27361 tgctgaggct caatggcgac ggtaaaggta acgcctttgc tggtgagttt acgatcaata
    27421 acctggccta cctgcacgcc gtgaagaatg agcggctgac cagcatcaat accgtaactt
    27481 tccggtgctg tcagggtcag tgtcagaaca tcaggttcat gcagcagtgc tttttcgcct
    27541 ggcacaacaa cgaactcttt gcgtggctcg ccatcgccag gcaccaactc aaaggttttt
    27601 ccggtcagca gggcgctgag attggcatcg ctaagggata atttcgggtt gcgtaattcg
    27661 atgcgggtat tttcccgtaa cagcgtaacg acgctgggat caacggtcat ttctccggtg
    27721 actttaccac caggatttaa atccagttta gtcagctgtc cgacttccag cccctgatac
    27781 attaacggcg tcgagtcggc ggttaatccg gccccactcg gcagttccag ttttattatt
    27841 acgccacgct ggctgtgggc cagatcttca tacagaccaa aggtatcttc cgcctcggca
    27901 ggtttcgact cttctggtga atcgaaggca atcgcaccgt taaccagtgc cgccagactt
    27961 tccagtttca ccttcgcgcc actgatactg acgttggcat caacgcctga aacgttccag
    28021 aaacggctac cttttttcac caggtcggta aaacgccgct cgatcaggac atcaatcacc
    28081 acgccttgtt tattgggatt gatggcatag tcgtagactt ttcccaccgg gatcttgcgg
    28141 aaatagacca atgaaccgct gttcagcgaa ccgagatcgg gagcttgcag gtggatcatc
    28201 agatcgccgt tatccagccg atacttcggt tgggtatcga gcgcgacaaa gtgatcctgc
    28261 tctttacctt tacctggcat catgccgata tagttaccac cgacgagggc gtccagcccg
    28321 gagacacctg ccaacgatgc ttttggcgtc accagccaga actgagtctc ttcgcgcagc
    28381 gcatctttca tatcggactt gatgctgacc ttgacttcaa tcttacgaag atcgtcgctg
    28441 aggctgatat cctgcactgt tccgacttca acgccctgat aacgaacagg cgtacggccc
    28501 gggacaatac catccgccga cataaagtcg atggtgacgg tattaccccg gtcctgataa
    28561 ctgtcccaaa tcagccaacc ggcaatcatt agcgcgatga acggcagcag ccagaaaggt
    28621 gagatacggc gtttattttt aatctgcgct tcagtcgtcg aagcgggcgt ttcctgactc
    28681 atgtgcatcc caaagtaagc ggctgtccag ccattccaca gcaagaatag tcaaaattac
    28741 cgctgcgccg aaataaaacg cagccggtcc catagtaaaa gcgaggatct gatcgcgatt
    28801 aatcagcgac atggttaaag atatgacaaa caggtcgagc atcgaccagc gaccaatcca
    28861 ggtcaccatc cgcagtaaca gaatgcgtgt gcgtaaacct tgctggcatt taaaatgaat
    28921 gctcaacagt aaagtgaaca tgacgatcac tttagtaaac ggtaccagaa tactggcgat
    28981 aaacacgatt cctgcaaccg caatgttgct acttgccagc gacataattc cggaaagaat
    29041 tgtatcttcc tgccgtcctc cgttcagata aatgatagaa ataggcaaca ggttggcagg
    29101 taacaataaa acgattgacg ctaacagcgc cgcccagcat ttttgcagac tatgacggcg
    29161 acgcaggcgt agcggaatat gacaacgcgg gcagcgacca cgctgatcag gatagccggt
    29221 aaaatggcac ccaagacaga cacgaagttt ctcgtcccta cgcgtagcgg ggcgctgcgg
    29281 ataaaatcgc tcccacagct cctcgacatt aagatgtgac aacgtcaccg tcgttaaaat
    29341 caccaacgcc acaaaagaga acaagccgac acccgcctgg atatgggcat aatcctgtac
    29401 ctttatagaa gcaacgccaa tgccgaccag gtagatatcg agcattaccc actctttaag
    29461 tcgctcaagc atcagcagta ccgggcgtag attcattccc agtcggttac caaaccataa
    29521 ataagctatg gaggtcacca gaatgagcgg ggcaccaata acgcagaaaa agaccatcga
    29581 ccccgttatc gcatcgccct gtttggtcat ttgccagatg ccttgcataa cgttggcgtc
    29641 aatacgaatg cctaacagcc agatatgcaa cagcggttcg ccccaggcaa acggcatcag
    29701 caacagcatg gtgaaggcca ttgccgccag gcgcgttagc gaccagtcgc gcccgtcacg
    29761 aatttttgcc tgacagcgcg gacaataggc actttgatga gaatttatct cgggcaggct
    29821 aaacagcatg tcacattgcg ggcaacgttg gtaatcacca cgcggcagtt cctcgccgat
    29881 tgccctcact gttatctttt ttgtcggcgt aatttgtggt gtgttaagag ccatgacgtg
    29941 ccaaatgtaa taagaattaa cattaatctt aacccatgat gggaatgatc ttgagcatta
    30001 acgcatttaa tgcttatttt aataggctgg cagttccagg taagacaacc aagtgattat
    30061 ataatgaaca aaacagaatt ttacgcggat ttaaatcgcg actttaacgc gctgatggcg
    30121 ggagaaacca gttttctggc aacgcttgcg aacaccagtg cgttgttata tgagcgtctc
    30181 actgacgtaa actgggcagg tttttatttg cttgaggacg atacactggt gctcggacca
    30241 tttcagggca aaattgcctg tgtccggata cccgtcgggc gtggcgtgtg cggcactgcg
    30301 gttgcccgca atcaagtgca gcgtatcgag gatgttcatg cgtttgacgg gcatattgcc
    30361 tgtgatgcgg cgagtaattc tgaaattgtt ctgccgctgg tggtgaaaaa tcagattatt
    30421 ggtgttctcg acatcgatag taccgtcttc ggtcgcttta cagacgagga cgagcaaggc
    30481 ttacgtcagc ttgtggcaca gcttgaaaaa gtgcttgcaa cgacggatta caaaaaattc
    30541 tttgcgagcg tcgcaggata atcaacggat aacgtagcat ttactgatag cgtcattata
    30601 atgacgcctg ttcatgcctg cgcttgttgg ctacgtccgt tgtaatcagg aaatttcatg
    30661 gaaaatcaac ctaagttgaa tagcagtaaa gaagtaatcg cgtttctggc cgaacgtttt
    30721 ccccactgtt tcagtgcgga aggtgaagcg cgtccgctga aaatcggtat ttttcaggat
    30781 ttggtcgatc gtgttgccgg ggaaatgaac ctgagcaaaa cgcaattacg atccgcttta
    30841 cgtctctaca cttcgagctg gcgttacctt tacggtgtta aacccggcgc aacgcgtgtc
    30901 gatcttgacg gcaacccatg cggtgagctg gacgagcaac atgtagagca tgctcgcaag
    30961 cagcttgaag aagcgaaagc gcgtgttcag gcacagcgtg ctgaacagca agcgaaaaaa
    31021 cgcgaagctg ccgcagctgc tggtgagaaa gaagacgcac cgcgtcgcga acgcaagcca
    31081 cgtccgacca cgccacgccg caaagaaggc gctgaacgta aacctcgtgc gcaaaagccg
    31141 gtagagaaag cgccaaaaac agcaaaagca cctcgcgaag aacagcacac cccagtttct
    31201 gacatttcag ctctgactgt cggacaagcc ctgaaggtga aagcgggtca aaacgcgatg
    31261 gatgccaccg tattagaaat caccaaagac ggcgtccgcg tccagctgaa ttcgggtatg
    31321 tctttgattg tgcgcgcaga acacctggtg ttctgaaacg gaggccgggc caggcatgaa
    31381 catgtttttt aggcttaccg cgttagctgg cctgcttgca atagcaggcc agaccttcgc
    31441 tgtagaagat atcacgcgtg ctgatcaaat tccggtatta aaggaagaga cgcagcatgc
    31501 gacggtgagt gagcgcgtaa cgtcgcgctt cacccgttct cattatcgcc agttcgacct
    31561 cgatcaggca ttttcggcca aaatctttga ccgctacctg aatctgctcg attacagcca
    31621 caacgtgctg ctggcaagcg atgttgaaca gttcgcgaaa aagaaaaccg agttaggcga
    31681 tgaactgcgt tcaggcaaac tcgacgtttt ctacgatctc tacaatctgg cgcaaaagcg
    31741 tcgttttgaa cgttaccaat acgctttgtc ggtactggaa aagccgatgg atttcaccgg
    31801 caacgacact tataaccttg accgcagcaa agcgccctgg ccgaaaaacg aggctgagtt
    31861 gaacgcgctg tgggacagta aagtcaaatt cgacgagtta agcctgaagc tggcaggaaa
    31921 aacggataaa gaaattcgtg aaaccctgac tcgccgctac aaatttgcca ttcgtcgtct
    31981 ggcgcaaacc aacagcgaag atgttttctc gctggcaatg acggcgtttg cgcgtgaaat
    32041 cgacccgcat accaactatc tttccccgcg taataccgaa cagttcaaca ctgaaatgag
    32101 tttgtcgctg gaaggtattg gcgcagtgct gcaaatggat gatgactaca ccgttatcaa
    32161 ttcgatggtg gcaggtggtc cggcagcgaa gagtaaagct atcagcgttg gtgacaaaat
    32221 tgtcggtgtt ggtcaaacag gcaagccgat ggttgacgtg attggctggc gtcttgatga
    32281 tgtggttgcc ttaattaaag ggccgaaggg cagtaaagtt cgtctggaaa ttttacctgc
    32341 tggtaaaggg accaagaccc gcactgtaac attgacccgt gaacgtattc gtctcgaaga
    32401 ccgcgcggtt aaaatgtcgg tgaagaccgt cggtaaagag aaagtcggcg tgctggatat
    32461 tcctggcttc tatgtgggtt tgacagacga tgtcaaagtg caactgcaga aactggaaaa
    32521 acagaatgtc agcagtgtga tcatcgatct gcgtagcaat ggtggtgggg cgctgaccga
    32581 agcggtatcg ctctcaggtc tgtttattcc tgcgggtcct attgttcagg tccgtgataa
    32641 caacggtaaa gttcgtgaag acagcgatac cgacggacag gttttctata aaggcccgct
    32701 ggtggtactg gttgaccgct tcagtgcttc ggcttcagaa atctttgccg cggcaatgca
    32761 ggattacggt cgtgcactgg ttgtgggtga accgacgttt ggtaaaggca ccgttcagca
    32821 ataccgttca ttgaaccgta tttacgatca gatgttacgt cctgaatggc cagcgctggg
    32881 ttctgtgcag tacacgatcc agaaattcta tcgcgttaac ggcggcagta cgcaacgtaa
    32941 aggcgtaacg ccagacatca tcatgccgac gggtaatgaa gaaacggaaa cgggtgagaa
    33001 attcgaagat aacgcgctgc cgtgggatag cattgatgcc gcgacttatg tgaaatcagg
    33061 cgatttaacg gcctttgaac cggagctact gaaggaacat aatgcgcgta tcgcgaaaga
    33121 tcctgagttc cagaacatca tgaaggatat cgcgcgcttc aacgctatga aggacaagcg
    33181 caatatcgtt tctctgaatt acgctgtgcg tgagaaagag aataatgaag atgatgcgac
    33241 gcgtttggcg cgtttgaacg aacgctttaa acgcgaaggt aaaccggagt tgaagaaact
    33301 ggatgatcta ccgaaagatt accaggagcc ggatccttat ctggatgaga cggtgaatat
    33361 cgcactcgat ctggcgaagc ttgaaaaagc cagacccgcg gaacaacccg cacccgtcaa
    33421 gtaatatcaa tcaggcacaa gaaattgtgc ctgatttttt aacagcggca agatgccgta
    33481 aatcagatgc tacaaaatgt aaagttgtgt ctttctggtg acttacgcac tatccagact
    33541 tgaaaatagt cgcgtaaccc atacgatgtg ggtatcgcat attgcgtttt gttaaactga
    33601 ggtaaaaaga aaattatgat gcgaatcgcg ctcttcctgc taacgaacct ggccgtaatg
    33661 gtcgttttcg ggctggtact gagcctgaca gggatacagt cgagcagcgt tcaggggctg
    33721 atgatcatgg ccttgctgtt cggttttggt ggttccttcg tttcgcttct gatgtccaaa
    33781 tggatggcat tacgatctgt tggcggggaa gtgatcgagc aaccgcgtaa cgaaagggaa
    33841 cgttggctgg tcaatactgt agcaacccag gctcgtcagg cggggatcgc tatgccgcaa
    33901 gtggctatct accatgcgcc ggacatcaac gcttttgcaa ccggtgcgcg ccgtgatgcc
    33961 tctctggttg ctgtcagcac cggtttgctg cagaacatga gccaggatga agccgaggcg
    34021 gtaattgctc acgaaatcag ccacatcgcc aatggtgata tggtcaccat gacgctgatt
    34081 cagggcgtgg tgaacacctt cgttatcttt atttcccgta tcctggcgca gcttgccgcg
    34141 ggttttatgg gcggaaatcg tgatgaaggt gaagagagca acggcaaccc gctgatctac
    34201 tttgcggttg caacggttct ggaactggtg tttggtattc tggcgagcat tatcaccatg
    34261 tggttctcgc gtcatcgtga attccatgct gatgccggtt cggcaaaact ggttggtcgc
    34321 gagaaaatga ttgccgcgct gcagcgcctg aaaaccagct atgaaccgca agaagcaacc
    34381 agcatgatgg ctttctgcat taacggtaag tcgaaatcgc tcagtgagtt gtttatgact
    34441 cacccgccgc tggataaacg tattgaagct ctgcgtacgg gtgaatacct gaagtaatat
    34501 gaagtaaata ctaaaaagcg cgtcctgctc gacgcgcttt ttttatgccc tggatcttgg
    34561 ctgagtgata cgtaacccac tgatacaggc agcaatcact gccagaatag ccgcagccat
    34621 cagcgagacg tgtgtaccat tatctccaaa ctgatttagc atcagcgcca ccagcgccgc
    34681 gccgctactc tgacccagta gacgagccgt tcctaacatg ccactggctc caccgctacg
    34741 ttcgcgaggc gcggaggtaa taatggtgtg gttattgggt gactggaaca agccaaatcc
    34801 agcgccacat aagatcatcg gccagataat attgatatcc gcaggtgacg cgggcagcag
    34861 aaccagggaa aaaagccccg cagccatgat gaataatcct aaagcaccca gcaatcctgc
    34921 atgtacgcgt tcaatcaaat agcctgccaa cggagccatc accatcgttg ctaacggcca
    34981 cggtgtcaga agtaaacctg tttcgacttc actacgcccg agcacggttt gcaggtaaaa
    35041 gggcagggaa accattgcca gcatttgtgc gcagaaagag caaacagatg tgcaaataga
    35101 aagtgaaaac agcgggatac gcagtaaatc caccggtagc agcggtactg gaagagaaag
    35161 ctggcggcga ataaagaaaa taccaacgac aaccattaca accagttccg cagcaattaa
    35221 cgtcagcgat tgcccctgag cgaaaccact caacgcagtg ataagcaggc cgaaggttaa
    35281 cgcgttcatc acggcgctgg gcaggtcgaa acggggttta ctggcgcgag aaccattggg
    35341 tggcagaaaa cgcatcgcca gaagcagggc gataataccc aacggtacgt tgattaaaaa
    35401 taaccatttc caggatgcga tggagaggat tgctgcagca attgtcggcc cggcagcaga
    35461 agagacggca acaataaacg agtttatgcc catccctcta cccagaaaac gttgtggata
    35521 gatcaggcgg ataagggcgg tattaacgct catcaacgct gcaccgccga aaccttgtat
    35581 gacacgcgca agggtgagta tttgcagcga atcagaaagg gcgcagaaca gtgaagacaa
    35641 cagaaaaacg accagaccgc atttataaat acgtcgatag ccaaacatat cacccagaaa
    35701 cgaaaacgag agcagggaga tgacaatggc gatttgatag gcgttcacta cccagatgga
    35761 actggctgga gtggcatgaa ggtccgtggc gattgttggc agggcgacgt tggcgattgc
    35821 gccgtcaagg acagccatcg aaataccaat cacaatggtt aatatcgcac cgtatcgctg
    35881 gggcaatggc aggccgtcgg cctgaacttt tggcatagga attttaaatc tttggtgaat
    35941 aatcaacagg ggtatgattc tagcactgat attcacggtg gatgtcgcag aattgtaact
    36001 aaccggcaaa agatggattg cgaccaccct ggtgcgaaat tataataaaa aacagttctg
    36061 atttttataa aacactcgca atgaggtgat aaatggctaa cgcagatctg gataaacagc
    36121 ctgattctgt atcttccgtg ctaaaagttt ttggcatttt gcaggcgctg ggtgaagagc
    36181 gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat gtcaaaaagc accgtttatc
    36241 gctttttaca gaccatgaaa accttaggtt atgtggcgca ggaaggggag tcggagaaat
    36301 attccctgac cctgaaattg tttgaactgg gcgctcgcgc gttacaaaac gtcgatttaa
    36361 ttcgtagcgc agatatccag atgcgtgaga tctcccgcct gaccaaagaa actatccacc
    36421 tcggcgcact ggacgaagac agtattgttt acattcacaa aattgactct atgtacaatt
    36481 tgcgcatgta ttcacggatt gggcgtcgta atccactgta cagcaccgcg attggtaagg
    36541 tactgctggc atggcgcgat cgcgatgaag tgaagcaaat tcttgagggc gtggagtata
    36601 aacgcagtac cgagcggacc atcaccagta cagaagcgtt attaccggtt ctggaccagg
    36661 tgcgcgagca ggggtatggc gaagataacg aagagcagga agaagggctg cgatgcattg
    36721 cggttccggt atttgatcgc tttggcgtgg tcattgccgg tttgagcatc tccttcccga
    36781 cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc aatgttgcat accgcagcgc
    36841 gcaaaatttc tgcccaaatg ggttatcacg actatccgtt ctgatgagag taagaacctg
    36901 tcggaatatc aaacagacag gttctttatt tagcatgaga aaaataaagt tgaaggtggc
    36961 gttagattaa acgcagttga tataagaata ttttactcat gagtgagaat ctggttattt
    37021 attacactta accattatcg accacgatat tgcttttgcg taacagcggg caatctgtta
    37081 tccccaaaaa accactttta gtgtgcaagt attgtaccgt gctggtgcct ctggcagtca
    37141 gataggtaca ttgcaaacct aattctgcgg cattctcttt gcttccaatc aaaacgccat
    37201 atccgctgag taataatcct atccatacca gtgctatcag cataactgtg cgaatgatga
    37261 atcgcattac aacctcttct cttttttatg ttcgcttaat cgtagcggca atatgcgctg
    37321 aagcaagcga ctcattccga aaaagcacga atatcgacat agttagacgc tgtttaacta
    37381 acgcatggta gtttaatgac ataaggtagg tgaaacggag attggagtga aaaagtttcg
    37441 atgggtcgtt ttggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa
    37501 catgatgtgc gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat
    37561 cccgtggtga tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata
    37621 gcgcctctac tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt
    37681 gcgtctttgg ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatt
    37741 aatcgtgcca gttctcggac taacgaacag attgaactgc ttgaggcgtt gctggatcag
    37801 caaaaacgtc aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca
    37861 gataaaaaga ctattgagag tcaaaaatcg gttgaagacg aagatattat tcgcctggtc
    37921 gccgaacgat aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc
    37981 atcctgatta tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt
    38041 caaataatta tcatattatt tatttg
//
LOCUS       DAZFTN010000040        37521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFTN010000040 DAZFTN010000000
VERSION     DAZFTN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHKV2_002168"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHKV2_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO6940049.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHKV2_002169"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHKV2_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO6940050.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHKV2_002170"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHKV2_002170"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO6940051.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHKV2_002171"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHKV2_002171"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO6940052.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHKV2_002172"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHKV2_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO6940053.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHKV2_002173"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHKV2_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO6940054.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACHKV2_002174"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACHKV2_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO6940055.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHKV2_002175"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHKV2_002175"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO6940056.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACHKV2_002176"
     CDS             complement(8482..8526)
                     /locus_tag="ACHKV2_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940057.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACHKV2_002177"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACHKV2_002177"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO6940058.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACHKV2_002178"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACHKV2_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO6940059.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACHKV2_002179"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACHKV2_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO6940060.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10212..10610)
                     /gene="mutT"
                     /locus_tag="ACHKV2_002180"
     CDS             complement(10212..10610)
                     /gene="mutT"
                     /locus_tag="ACHKV2_002180"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO6940061.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10669..13374)
                     /gene="secA"
                     /locus_tag="ACHKV2_002181"
     CDS             complement(10669..13374)
                     /gene="secA"
                     /locus_tag="ACHKV2_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO6940062.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13436..13948)
                     /gene="secM"
                     /locus_tag="ACHKV2_002182"
     CDS             complement(13436..13948)
                     /gene="secM"
                     /locus_tag="ACHKV2_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO6940063.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14179..15096)
                     /gene="lpxC"
                     /locus_tag="ACHKV2_002183"
     CDS             complement(14179..15096)
                     /gene="lpxC"
                     /locus_tag="ACHKV2_002183"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO6940064.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15197..16348)
                     /gene="ftsZ"
                     /locus_tag="ACHKV2_002184"
     CDS             complement(15197..16348)
                     /gene="ftsZ"
                     /locus_tag="ACHKV2_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6940065.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16409..17671)
                     /gene="ftsA"
                     /locus_tag="ACHKV2_002185"
     CDS             complement(16409..17671)
                     /gene="ftsA"
                     /locus_tag="ACHKV2_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO6940066.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17668..18498)
                     /gene="ftsQ"
                     /locus_tag="ACHKV2_002186"
     CDS             complement(17668..18498)
                     /gene="ftsQ"
                     /locus_tag="ACHKV2_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO6940067.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18500..19420)
                     /gene="ddlB"
                     /locus_tag="ACHKV2_002187"
     CDS             complement(18500..19420)
                     /gene="ddlB"
                     /locus_tag="ACHKV2_002187"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6940068.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19413..20888)
                     /gene="murC"
                     /locus_tag="ACHKV2_002188"
     CDS             complement(19413..20888)
                     /gene="murC"
                     /locus_tag="ACHKV2_002188"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO6940069.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20942..22009)
                     /gene="murG"
                     /locus_tag="ACHKV2_002189"
     CDS             complement(20942..22009)
                     /gene="murG"
                     /locus_tag="ACHKV2_002189"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO6940070.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22006..23250)
                     /gene="ftsW"
                     /locus_tag="ACHKV2_002190"
     CDS             complement(22006..23250)
                     /gene="ftsW"
                     /locus_tag="ACHKV2_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO6940071.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23250..24566)
                     /gene="murD"
                     /locus_tag="ACHKV2_002191"
     CDS             complement(23250..24566)
                     /gene="murD"
                     /locus_tag="ACHKV2_002191"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO6940072.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24569..25651)
                     /gene="mraY"
                     /locus_tag="ACHKV2_002192"
     CDS             complement(24569..25651)
                     /gene="mraY"
                     /locus_tag="ACHKV2_002192"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO6940073.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25645..27003)
                     /gene="murF"
                     /locus_tag="ACHKV2_002193"
     CDS             complement(25645..27003)
                     /gene="murF"
                     /locus_tag="ACHKV2_002193"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO6940074.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27000..28487)
                     /gene="murE"
                     /locus_tag="ACHKV2_002194"
     CDS             complement(27000..28487)
                     /gene="murE"
                     /locus_tag="ACHKV2_002194"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO6940075.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28474..30240)
                     /gene="ftsI"
                     /locus_tag="ACHKV2_002195"
     CDS             complement(28474..30240)
                     /gene="ftsI"
                     /locus_tag="ACHKV2_002195"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO6940076.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30256..30621)
                     /gene="ftsL"
                     /locus_tag="ACHKV2_002196"
     CDS             complement(30256..30621)
                     /gene="ftsL"
                     /locus_tag="ACHKV2_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO6940077.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30618..31559)
                     /gene="rsmH"
                     /locus_tag="ACHKV2_002197"
     CDS             complement(30618..31559)
                     /gene="rsmH"
                     /locus_tag="ACHKV2_002197"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO6940078.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31561..32019)
                     /gene="mraZ"
                     /locus_tag="ACHKV2_002198"
     CDS             complement(31561..32019)
                     /gene="mraZ"
                     /locus_tag="ACHKV2_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO6940079.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32621..33625)
                     /gene="cra"
                     /locus_tag="ACHKV2_002199"
     CDS             complement(32621..33625)
                     /gene="cra"
                     /locus_tag="ACHKV2_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO6940080.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33707..33793)
                     /locus_tag="ACHKV2_002200"
     CDS             complement(33707..33793)
                     /locus_tag="ACHKV2_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO6940081.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33805..34296)
                     /gene="ilvN"
                     /locus_tag="ACHKV2_002201"
     CDS             complement(33805..34296)
                     /gene="ilvN"
                     /locus_tag="ACHKV2_002201"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6940082.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34299..36023)
                     /gene="ilvI"
                     /locus_tag="ACHKV2_002202"
     CDS             complement(34299..36023)
                     /gene="ilvI"
                     /locus_tag="ACHKV2_002202"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO6940083.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36341..37285)
                     /gene="leuO"
                     /locus_tag="ACHKV2_002203"
     CDS             complement(36341..37285)
                     /gene="leuO"
                     /locus_tag="ACHKV2_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO6940084.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9085 a        10449 c         9136 g         8851 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt aactattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac agggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagctttgc tggcttcgct tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggcagagct gactgctgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct
      601 gggtttctgc cgccgtgttc tttgtggtga ttaacgccat caacctgacc aacgtaacag
      661 tgtttggtga gatggagttc tggtttgcca ttatcaaagt tattgcggta gtagcgatga
      721 tcatcttcgg cggctggctg ctgttcagtg gtaacggcgg tccgcaggca agcgttagca
      781 acctgtggga tcagggcggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtatc ccgaaagcaa ctaaccaggt tatctatcgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaacgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgcgg cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg
     1261 tattgattaa ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat
     1321 ccgcactggt gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gtggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctgg tggtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc aaaagccgta aaagcacatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagcgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaatacctg atattgcgaa
     1801 gtacggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacctgc aagacgaacc ggcacccaat ccagtacatg aagcactgca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt attagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgacatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 cgccaatcaa cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattactg gaaggcacca acacgcagtt gttggatacg cgtaaaacct tacccggtct
     3601 gcgttcagcg ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaagc acgtacaggc actcgacctt tcaatgcgtt ttcgctagtt
     4021 gctgacagat ttatccgctc catagtgggc ggataatctc cccttcccca accttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaagcagct
     4141 ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcaccaac
     4201 caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacgccaggt tgggataacg
     4561 caaacggcgt caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac ggccctgtgc ctgcgttatc agggagtctt gctggatgcc agcgaagaag
     4741 tggttcatgt tgcggtggtc gatgccccct cacatgagtt gctggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctgactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgagccag gcactggatg ttaacacgct gggaatgcag ccgttacaac
     5281 tggcgggctt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 ccggcagcgg caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccgga ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccatta
     5581 aagccgcgca aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg
     5761 agcccatcca cattccagtc aatgtatggc cgtcgccgct gccccactgg caagcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgcactgc cttatttgaa gttctgccca
     5881 taacaccggt catacgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcccgaca ggcgggtatg cgaacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tattggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgta ctttactgct tatggcacta cagcaacaga tggttacccc actaagcctg
     6181 aagcgaattg ctatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctacttaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat ctctggcaca cgatctcgaa
     6361 cagggcattg ccttttccaa tgccttatta ccctggtcag aggtatttcc gccgctctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaagagccca gcgtcagttg accgacaaag taaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggggctggc tgctggtgtt gttcggcatt
     6721 atgctgacga tagccaataa gttgctgatg cgccgcccga cctggcttat cgcgcggcag
     6781 aaattgctgt tacgcatccc gattatgggg tcactgatgc ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gactcaaagt gcaggcatta cttttttaca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgttaaaaa atgccggtga gtttagcccg
     7021 ctctgtttgc aattagtgag aacgggagag gcctccggct cgctggatct catgttagac
     7081 aacatcgccc atcatcatcg ggataacaca atggcgctgg cggataacct agcagcctta
     7141 ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattcagc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccacc aaaggctttc gccacatcgc ccggcgtggt gcagccacca tcgctgatga
     7681 tcattccgcc cagaccgtgc gcagcatcgg cacattcgat taccgcagaa agttgcggat
     7741 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcggc acctgagagg ataagctcct cacacatttc accagtcact acgttaccag
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccacg tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtgcctacgg
     8161 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgcg ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa tatgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt tagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gactgccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
     9721 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    10141 gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg
    10201 gcgtgaacgc cttatccgac atacacacgc ttaagcttcg caattaccgg ttcattggct
    10261 ggcggaaaat catcggcatt aagaccgact agcgacatcc acttaccggg ttgcccttct
    10321 ttaccccacg gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg
    10381 tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact
    10441 tcttcctgaa gttcacgcac caccgcctgt tccggcgttt cacccatttc aattttaccg
    10501 ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt
    10561 tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac
    10621 ctataaaaaa gcgcagaatc ctgcgccttt tacttcaaca gttagctttt attgcaggcg
    10681 gccatggcac tgcttgtatt ttttaccaga accgcacggg caaggatcgt tacgtcctac
    10741 tttgcgctca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg
    10801 gctaagctgc tgcatttgcg ctaaacgctc ggcttccata cgacgctgtt gttccagctc
    10861 ctcaacctct tcaggcatac gtacctgaac tttgctcagc gtactgataa cttcatattt
    10921 caacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg
    10981 atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca tcgctgccag
    11041 gtgctctttc cacagggagt caagcgtttg cagcatgacg cctttctcga agtgacgcat
    11101 catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat
    11161 gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact cagcaattgg
    11221 taaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc
    11281 ttccagcgac tgtggcggaa tgtaggcatc aatggtcgct ttgaacacat cttcacgaat
    11341 gctgttaatg gtttcgctca catcgctgac atccagcaat tcgttacgct gggagtaaat
    11401 ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa
    11461 gttacgactt tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc
    11521 aatggcttcg cctggcttca tacccagttt acgcatcatg ccggatactc ggtcggaagc
    11581 aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac cagcatcccc
    11641 ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt gacgctcggt
    11701 accgatgata tgcaggccac ctgctgccag taccgcatcg tgacgtacct gccagtcggc
    11761 tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt ctgcctgcca
    11821 gctaccaccg agcacaatat ctgtaccacg acccgccatg ttggtcgcga tagtcaccgc
    11881 agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag
    11941 gacattgtgc ttaataccgg ctttggtcag ttcgtttgac accagctccg atttttcgat
    12001 ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat
    12061 gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat
    12121 cattggacgg ttggtcggaa caacgacggt atccagtttg tagatggagc taaattcgaa
    12181 agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac ggaagtagtt
    12241 ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc
    12301 ttccacagcc tggtgcagac catcggacca gcgacggccc tgcatggtac gaccggtgtg
    12361 ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcac gggtaaacag
    12421 cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta
    12481 cagagactcc ccttcatcca tgatgccctc tttcaccagc agttcttcaa tcagcaccag
    12541 accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc
    12601 ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac
    12661 gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc
    12721 ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac
    12781 acgttcttca gggctgaacg ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt
    12841 cgtaccgtaa gtgatgtcag ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg
    12901 caggttgata ccgacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc
    12961 acgttgcgcc aggtagtcgt tgacggtaac tacatgcacg cctttaccgg ttagtgcgtt
    13021 cagataagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttcggcgat
    13081 gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac gcataccaaa
    13141 gacgcgctta cttgcctcac gtaccacggc gaaagcttcc gggatcagat tttccagcac
    13201 ttcgcctttt tccagacgtg cacgaaactc tgcggttttc cctttcagtt cttcgtcgga
    13261 gagtttttcc atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg
    13321 cagggtgcga tcgttacgac taccgaaaac tttagttaat aatttgatta gcataataaa
    13381 atctcaaacg ccccgcgttg cggagttaat aaaatgaagt aaaggtttat tgttgttagc
    13441 tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga
    13501 attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc
    13561 cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag
    13621 gcaaagattc ttcagcaacg ggcagcgttt gcggtgccat tgcgaaggaa agatgacgga
    13681 ttaccgtgcg aatggcatgt tgatgccagt aatctacgga atagttcgaa ttcgggcggc
    13741 gcgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt
    13801 tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac
    13861 ctaaactcgc cgcaaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact
    13921 gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgtat tcaaacgtat
    13981 ttagcgcaca aatcttatca ttaaagcctg aagtccacag cacgaatgaa gataagttgt
    14041 ccgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatagctctt ataaaattca
    14101 aaaaaaaacg actggttcac ctggccggag agagtgccag atttgccagt cgaattttat
    14161 acgacagtat aaatgtcgtt atgccagtac ggctgaaggc gctttgaagg ccaacggcag
    14221 ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc tgtttcgcca ggacagcctg
    14281 cagcagtttg ttattcagtg catgaccgga tttataagcg gtaaatgcac caataatatt
    14341 gtgaccacac atgaacaagt caccgatcgc atcgagcatt ttgtgacgca caaattcgtc
    14401 ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg tcaacaacga tggcacaatc
    14461 gaagctgccg cccaggcaca aaccacggga ctgcagatat tcgatatcac gcatgaaacc
    14521 gaacgtacgc gcacggctga tctggcgcat aaacgcatca gcggagaagt tcatcgcata
    14581 gcgctggttg ctggaatcaa tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa
    14641 accattgtac ggcttaaatt cagcccactt atcgccatct tcgacacgaa cagtctcttt
    14701 gatgcgaaca aattttttgg cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta
    14761 tacaaacgga gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa cttcgataac
    14821 aatgttatcg atgcccaagc ccgcgagagc agcattgagg tgctctacgg ttgaaatccg
    14881 tacatcatgc tcgttgacca gacacgtaca gagcatggta tcacgcacag atttggcatc
    14941 ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc cggtgttggc
    15001 cggcgcaggg cgtaacgtca gggtgacttt cttgccggta tgtaaaccga cacccgtcgc
    15061 ctgaacgata cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc aaattaccta
    15121 tccaaccgaa gtgtactata cattcggcgg gccagtttag cacaaagagc ctcgaaaccc
    15181 aaattccagt caattcttaa tcagcttgct tacgcaggaa tgctgggata tccagataat
    15241 ccggctcttt cgcagtttgc ggcgcattgt cattcacgac tttagcaacc ggcttctgct
    15301 cctgggtcag cggagccatc ccatgctgct ggtagcgatc catcactggc tgctgaacct
    15361 gcttattggt caccagagtg atttcaggac gtttgtccat gccgatacct gtcgcaacaa
    15421 cggttacgcg cagctcgtca ttcatatccg ggtcaagaga agtaccgata accacagtcg
    15481 cgttgtcgga agcaaatgca cggatggtgt tacctaccgt ttcgaactca tccagacgca
    15541 ggtcgaagcc cgctgtgatg ttaaccagca cgccgcgtgc gccagacagg tcgatatctt
    15601 ccagcagcgg agaagagata gccatttcag cagcttcttc cgcacggtct tcaccgctcg
    15661 ccacgccaga acccatcatt gcgtagccca tttcagacat tacggtgcgt acgtctgcaa
    15721 agtccacgtt catcaaaccc ggacgagtaa tcagttcggc gatgccttgc acagcgcctt
    15781 tgagtacatc gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg cccagaactt
    15841 tcagcagttt gtcgttcggg atagtgatca gagagtccac atgcttggac agttcagtga
    15901 tcccctgctc cgcgaatgcc atacgcttct tgccttcaaa gttgaagggc ttagtgacga
    15961 cagcaacggt caggataccc aaatcttttg ccacttcagc gacgactggt gctgcacctg
    16021 taccggtacc accacccata cccgcagcaa taaagaccat gtctgcacct tccagcgccg
    16081 cacgcagtgc atcgcgatcc tcatctgccg cattgcggcc aacttctgga ttagcgccag
    16141 cgcccagtcc tttggtgata ccgctaccga tttgaatcgt ctgtccaacc gctgttttac
    16201 gcagcgcttg tgcatcggta tttaccgcga agaattcaac accttcaatg cgctcgcgca
    16261 ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt
    16321 cattggtaag ttccattggt tcaaacatag tttctctccg atttgtgcct gtcgcctgag
    16381 gccgtaatca tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa ctattgagtc
    16441 gcttgatcca cgagccaact gatgctgtaa cacgtttttc tacttcagct tcaccgttaa
    16501 gatgtgactc tttcccatag tgaagcaatc ccaccgccgt cgaataatac ggctcctgag
    16561 cataatccgt taaaccggta atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca
    16621 cgcgctgagc acaggctgca agaccttcga tctgcgctgc gccaccggtt aatacaatgc
    16681 ctgccgccag gtgatgttta accccttgtt ggcgaagctt ttcctgcaac tgcaatatct
    16741 cttcgttgac caggttgagc agctcggtat agcgcggctc gatcacctct gccagggtct
    16801 gacgttgcag actccgcggc ggacgaccgc ctacgctcgg cacttccacg ctctcatctt
    16861 ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg
    16921 gcggcgtgcc aaaggcgtaa gcgatatcac tggtcacgac attgccagca taaggaatta
    16981 ccttagtgtg gcgcaatgcc ccaccggtat aaacggcgat atccattgta ccaccaccga
    17041 tatcgacgac gcagacaccc agttcacgtt catcttccgt caataccgaa taacttgatg
    17101 ccagaccggc aaatatcagt tggtcaactt tcagcccaca acgttcaacc gctttgacga
    17161 tgtttttcgc catatcgttg tggcatgtga tcaggtgcac ttttgcctgc atccgcacgc
    17221 ccgaaagccc taccggattc ttgatccctt cctgatagtc aatcgcatac tcttgcggga
    17281 tcacatgcag cacacgatgc tcatcgcgta cacgcaccga tttcgcggta tggacgacgt
    17341 tttccacatc ttcttgcgtt acttcttctt cagaaatagg caccatacca atttcattct
    17401 ggcagctgat gtgcttacca gaaagcgcca gatataccga agagatctga caatctgcca
    17461 tcaattctgc ctggtcaatg gcacgttgta cgcacttgac cacggattcg aggtcgttca
    17521 ccccgccttt atccatacca cgcgacgggc agctgcccac gccaatgata ttgaccatac
    17581 cgtcgggcag aacttcccct actaaagcgg caaccttcgc ggtaccaatc tccagtccta
    17641 ctaccagttt tctgtccgtc gccttgatca ttgttgttct gcctgtgcct gattttgttg
    17701 ctgagtagat tcctctggcg gcaagggcgc ccagcctact gccgctccag agtcataacg
    17761 caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta gaaccggata
    17821 aagttctaca aagcgagcca aacgtttcat cgtatcgccc cggccaagat tgagcttaat
    17881 atcgttattc agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt
    17941 aaatctgtcc tttgccagca tctgccccat ttcgcgatag ccctgcaaca cttcattggc
    18001 gctgccttcc gggccataca gcattggaag cacctgcttg ctggtgcgat ctggcggcac
    18061 gctgaaggta tttccttccg cgtctaccat gtgttgatca ttccaccgcg caatcggcac
    18121 atattcaacc agatgaatct tcaattcatc aggccactgc tttctgacgc tcacctgctt
    18181 aatccacggc agacgttgtt ctatttgcgt ctggatgatg ttgacatcct gggtcataaa
    18241 ggtacccggc tcacccaatg ccaggatcga ctgccggata tcgtcattac gcgtgtaatg
    18301 gcgttcaccg gtcaacacca gctttgagag cggcaggcgt tgcgcatctt ccatccagcc
    18361 caacacgacc cagccgctca ccaacactgt cgttaaaacg gtcagcagga aaaggatccc
    18421 cgccagacgc gttccattat tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt
    18481 cagagcagcc tgcgacatat tagtccgcca gttccagaat tcgtactacc aactgcgaga
    18541 agctcatacc tgcctgacgt gccgccatcg gcaccaggct gtggctggtc atacccggtg
    18601 aggtattggc ttccagcaga taaaactgtc catcgctgtc cagcataacg tcaatacgtc
    18661 cccatccttt gcaacctaac gtcgtccatg ctttcagcac taatgcctgc aaattggcct
    18721 cttgtgacgc ttccagacct gcggggcaga aatactgtgt ctcatcagag agatacttcg
    18781 cctcataatc atagaaggtt ccggacggtt gaatacgtac tgacggtaaa atttcttcac
    18841 cgagtatcgc aaccgtgaac tccggcccac ttagccattt ttcaatcaat acttcttcat
    18901 cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt ttctgctact acttttgaca
    18961 ttcccacact ggaaccttcg cggctcggct taacgataac cggcaaaccc agagcagaaa
    19021 tttctgctaa ctgcttatca ctcaggcctt tttcaaactc tacgcgggtt aacgctaccc
    19081 acggcgcgac cggtaaaccg gcaccttgcc acagaagttt gctgcgtagt ttatccattg
    19141 aaagcgcaga tgccatcact ccgctgccgg tataaggcaa gcccatcagc tcgagcatcc
    19201 cctgcagcgt accatcttca ccgccgcggc cgtgtagcgc gataaacact ttctgaaagc
    19261 ccatcgactt cagttgcgtc acgtcaactt ctttcgggtc gacaggatat gcgtcaatac
    19321 cgccttcacg cagcccggct aacaccgctg cgccagaatt cagagaaact tcccgctcag
    19381 cggaagtccc gcccaacagg accgcgattt tatcagtcat gttgttcttc ctccggagtt
    19441 tgcggcttca gtttgatttc agctaaagaa cgggcaattt ttccgatatt accagccccc
    19501 tgaacgagaa tcaggtcgtt accggttaat accggtgcca gcatctcggc tacctgcgca
    19561 gggtccggca ccagaatggg atctattttc ccacgtccac gaattgtgcg acacagcgaa
    19621 cggctgtccg cacccggaat tggcgcttcg ccagccggat acacttccag catcaacagg
    19681 gtatcaacct gcgtcagcac attggcgaaa tcatcataca ggtcgcgtgt acgggtaaaa
    19741 cggtgcggct gaaacagcat taccaggttt ttatccggcc agcctgcgcg cgccgcttta
    19801 atggtggcgt ccacttccgt cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg
    19861 cttttaccat tcactggctc cagcgggaat tcaccgagga aatcaaaacg gcgaccagtc
    19921 ccctggaagc tctccagcgc acgcaaaata gcctcgtcgt caatgccctc ttccgtagca
    19981 accgcaaccg cagctgcggc gttcagcgcg ttatgacggc ctggcgcatt cagggtgacg
    20041 cgcatcggct ctttgtcctg gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga
    20101 taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt aagttgtggt ctgacgtccc
    20161 actcgcggta acaattcgcg gatcaccgga tcatcaacac acatcaccgc acgaccgtaa
    20221 aacggcaggt tgtgcagaaa attaataaaa gtctgtttta aattctcaaa gtcgccctgg
    20281 taggtatcca tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc
    20341 aggaacgatg catcactctc atctgcttcg gcaatcaggt accgaccatg ccccaaacgc
    20401 gcatgaaccc ccgccgcttt taccagcccg ccgttaacga aggttgggtc gagccccgct
    20461 tctgcgtaga tgctggagac catcgcggta gttgtcgttt tgccgtgcgt tcctgcaatg
    20521 gcgatgccat gacgaaaacg cattaactca gccagcattt cggcacgacg gatcaccgga
    20581 atacgcgctt catgagctgc gacaatttcc gggttatcgg cagaaatggc gctggaaaca
    20641 acgaccacgc tggcatcacg tacgttttcc gggcgatggt tgaaataaat cgtcgctccc
    20701 agattcatta actgctgcgt gaccggattt ggcgctaaat cggaaccact gatctgataa
    20761 ccttcattgg ccagaacttc ggcaataccg cccataccgg caccaccgat gccgacaaaa
    20821 tgtatgtgcc gaacgcgacg catttcgggc acgatggaac gcagttttgc caattgttgt
    20881 gtattcattc tttacgccat taacttctta aattcatacg atgcaaaagg catcgctaca
    20941 attacgcccg ggcagcccga ctcacttcat ttgccactcg ctcggtggca tccggaatgg
    21001 atgcagcgcg ggcgcgttct gccatggtta ataaggtttc tcgcgaccac ccggccaggg
    21061 tgttggcgac agcatccacg ctaagctgtg gctgctcgat aattttggct gcgcccgctt
    21121 tttccagcgg tagcgcattc cagtattgct ggcggtcttt atgttgaaac ggcacaaaca
    21181 acgccggtag tcctgccgcg gcgatttcac tcaccgttaa agcaccggag cggcaaacga
    21241 cgacatccgc ccacgcatac gccgccgcca tatcatcaat aaattcagtc actttatgct
    21301 gcggttgccc cgcttcggca tacgcctgtt caacggattg ttgcgaacct ttgccgctct
    21361 gatgccagat agtgactgaa tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa
    21421 gaatgcgtgc gccctgggaa ccacccacta ccagcacacg aaccggacct tcacgtccag
    21481 ccaaacgttg ctgcggcaac ggtagcgcca acacatcggt acgcaccggg ttacccacca
    21541 cttccgcatt ggggaaagca cctggaaacg cctgcatcac tttggtggca atcttcgcca
    21601 gccatttatt ggttaagccc gcaataccgt tttgttcatg aagtacaacc ggaatgccta
    21661 acgaccacgc ggccagacca ccaggacctg atacgtagcc gcccataccg agcaccacgt
    21721 caggtttgta cgctttcata atcgcccgcg cctgacgcca ggcattgaag atacgcagcg
    21781 gcgcagctat cagtgctttt ataccttttc cacgcagacc agagatacga atgaaatcaa
    21841 tttcgatgcc atgttttggc actaagtccg cttccatacg gtcggcagtc cccagccagc
    21901 gaacttgcca accctgagcc attagatggt gcgcaaccgc cagtcccggg aatacatgtc
    21961 caccggttcc gcctgccatc accattaatc gctttccttg agcactcatc gtgaacctcg
    22021 tacaaacgcc tgcgctttct ccagacgcgt ttcataatca atacgcaaca gcatcatgat
    22081 ggctgtcgac ataatcagta agctcgaacc accgtaactg atcagcggca atgtcagacc
    22141 tttggtcggt aacatccccg ccgccgcgcc tacgttaacc agcgcctgga agctaaacca
    22201 gatgccaata gaacaggcga gaaaaccgga aaaacggtgg tcaatttcta atgctttacg
    22261 gccaatcgac atcgcgcgaa aagcgacgaa gaataccatt aaaagtgcca gcaccacacc
    22321 gacatacccc agttcttcgc cgataatggc gaaaataaag tcagtgtgcg cttccggcag
    22381 atactccagt ttttgtacag agttacctaa accttgtccc cacagttcgc cgcgaccaaa
    22441 cgccatcagc gattgcgtta actgatagcc gctgccaaag ggatcttccc acgggttcca
    22501 gaatgcggta acacggcgga tacggtacgg ttcggcgagt atcagcaaca caaccgctga
    22561 aatgcccata ccgataatgg caatgaactg ccacaatttc gctcccgcca ggaacaacat
    22621 cgccagcgta gtcacaaaca acaccaccac cgtaccaagg tctggctgtg ccagcagtaa
    22681 cactgccaac accagaatca cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac
    22741 ttcgtcacct ttacgcacca gatagttggc gatatagcaa aacagcgaca gttttgtcag
    22801 ctcagcaggc tggatacgca gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga
    22861 gctccccact accagcacga tcatcagcag gatgatagat ccgagcagca tcgtggcact
    22921 gtagcgttgc cagaactcca tcggcagacg cagcgtaatg atcgccagaa taaacgccaa
    22981 aatcagatag acaccatcac gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc
    23041 tatgggcatt gacgccgagg tcaccatgat aaagccaatc gccgcgaggc cgaaggtcag
    23101 ccacagtaag gtgcgatcgt acatgatcag gctgtcggta tctttttccc gcgagcccat
    23161 cacccagccc tttagcgccg tggagatcca gaccaggata ctgaatcctg gcaggcgcgg
    23221 cattttcagg cgagggagag ataaacgcat caacctaact ccttcgccag acgggcaaac
    23281 tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc cggggagagt
    23341 agaaccatat cgcccggctg aacacgcgga gccagcaagc gcatcgcctg ttccatagtt
    23401 tcggtttgtt ctgccacttc cgggcgtagc gccgccagct gcacgccgtc acgaccgaaa
    23461 caatacagac gtacgttatc gccattcagg taacgcgcca gtgggctaaa gtccgccgat
    23521 ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta cgtgcaggcc attcagcgcc
    23581 gcttccgtac tgccgacgtt ggtcgctttc gaatcgttaa tccaacgtac gccgttatgc
    23641 tccagcacaa cttcaaagcg atgcggcaga ccagtgaatg tggttaacgc tttcaggctg
    23701 ctggcacgcg gtaaccctgc ggcatctgcc agcgccagcg ccgccagcgc attggtgtag
    23761 ttatgctgcc cggaaagttt catttctttc acgttcagca ctttctcgcc tttcacccgc
    23821 agccaggttt cgccctgctg atgattcagg tgatagtcac ccatgttgac gccaaagctg
    23881 acgcagcgtt catccgcacc gcgaatcggc attgttaagg catcatcagc attgacgaca
    23941 caaactttcg cattttcgta aatacgcaat tttgccgcac ggtattgctg taaaccaaac
    24001 ggatagcgat ccatatgatc ttcagtcacg ttcagaatgg tcgctgctac cgcctgtaag
    24061 ctggaggtgg tttccagctg gaagctcgac agttccagta cgtacagttc gcactcatca
    24121 tccagcagca tcaacgcggg caggccaata ttgccaccca caccaacgtt aacccccgcc
    24181 gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc
    24241 gccacaatcg gtgcttgtgc ttcgcgacag aacagctcga tatcgccaac gatttcgatt
    24301 ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca
    24361 atcagatctg ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct
    24421 tcgggtaatt tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca
    24481 ccgcgagcga ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc gataatgaca
    24541 acatttttac cctggtaatc agccatgatt aacgtacctt cagcgttgcc agaccaatca
    24601 ggaccagcat cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt
    24661 tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa
    24721 aggagccgac ctgcaggatg acggaaagcg tttctaccac gaacacgccc cccataatca
    24781 ccagcaggaa ttcctgacgc agcagtacgg cgataatgcc taacgcacca cctaacgcca
    24841 gcgaacctac atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca
    24901 gccctgcccc gactatcgcg gtacagacaa taaccagttc cccggcgtgt cgcagatatg
    24961 gtatgtgtaa atagctggca aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac
    25021 caccggcgac aaatacggtc ggcataattg ccagaccgtc aagaccatcg gtcaggttta
    25081 ccgcgttgcc agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagtt
    25141 gcggcatcac atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccaa
    25201 caaggtacag cgcgaaggcg acacccagcg caatgaccga catccagaaa tacttccaac
    25261 gagcgatcaa ccctttggtg tctttacgca ctactttgcg ataatcatca acaaagccaa
    25321 taacaccgta acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag
    25381 cccacagcag tacggagatc acaatcgccg tcaggatcat aatcccgccc atggtcggcg
    25441 taccgcgctt gctgaagtgt gattcaggac cgtcgttacg caccacctga ccaaaggaaa
    25501 gtttttgcaa atgagcaatc atacgcgggc ccatccacaa tgagatgaac agcgcggtca
    25561 gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt
    25621 tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaaag cgcgtactac
    25681 ctcttccatg gcggcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc
    25741 aatcagtgat ttaagacgcg taattaacgc agttttctca gcaaaatgtt cgccaacgcc
    25801 gctggcggtg ctgatagcat ggctttgttt acccacgctt aacacacggt caataccagc
    25861 agcttttgcc gcctcgccca cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc
    25921 catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct gaacagcggc
    25981 ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag
    26041 ttgaatgggg aacaaacggc ctggaacagc tttcagattt gccagccccg ctttgatagc
    26101 atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt
    26161 gtgacgcccc ggcaacggca gcagaacatc cacgctaccg gttggggttt gcagggtaaa
    26221 ttccgtaccg tgcgaagtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt
    26281 aggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt
    26341 gttgtcggcg ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt
    26401 cgctttcgcg acacccgcaa gcgagccaaa accttccaga tgcgccgctg ccaggttgtt
    26461 gaccagcgca gcttccgggc gagtcagact cacagtccag acaatttcgc cctgatggtt
    26521 cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg
    26581 tacaccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa
    26641 aatcgccgcc gtcatctctt taacggaggt tttgccggag gaacctgtca gagcaaccac
    26701 gcgcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc
    26761 cttgacgatt aactgcggca ggtcgatatc cagcggacgg ctaaccagta gtgcgcctgc
    26821 gccgccagct ttcgcctggt cggcaaaatc atgagcatcg aaacgttcgc ctttcagggc
    26881 aacaaacagg cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat
    26941 atctgcacct tgcagttcac cgttgagaat gtcggtaagt tggctaaggg ttacgctaat
    27001 catgcaatca cccccagcag acgcgccacc gtgacgcgat cggagtagtc cagacgctga
    27061 ttgccaacaa tctggtaatc ttcatggcct ttgcccgcga ccagcaccac atcgttctct
    27121 ttagcctgca taacggcgca agtcaccgct tcagcacggt cttccatcac tttggcatgt
    27181 ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggctc ttcggtacgc
    27241 ggattatcgt ccgtcaccac cgccacatca gcaaactctt cggcaattgc gcccatcagt
    27301 ggacgcttac ctttatcgcg atcgccacca cagccaaaga cacaccacag cttgcccgca
    27361 cagtgcagac gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc
    27421 accaccaccg tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc
    27481 agacgagcag cggttttcag cagatcagcc agtggatagc ccagcgccag cagcgtcgcc
    27541 agcgcgagca gcaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca
    27601 tcgccccaac ttgagctaaa gcgaatagtc gcaccgctgt cgtgatagtt cacttcggtc
    27661 gctttcaacc agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaact
    27721 gcatccggca gttttgccag ccagcggcgg cctacttcat cgtcggcatt aataatcgcc
    27781 tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca
    27841 ccatgataat caaggtgatc gcggcttaag ttagtaaaga ccgacgccgc aaatttcaat
    27901 gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaaagtcgcg
    27961 tcctgatcca ccagccccgc cagctcatgc tgaacatcaa ctgccgaacc ggtggtattt
    28021 tctgtcggga tcactttccc cagcagaccg ttaccaacgg tgcccattac cgcgctggtt
    28081 tcgccaagca gttggctcca ctgcgccagc agctgggtcg tcgtggtttt gccgttggtg
    28141 cccgttacgc ccacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt
    28201 gcagataaac gctcgttgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg
    28261 atttcaccgt cggtcgcctc acctttcgcc tctgcaataa tggcagccac accttgcgct
    28321 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    28381 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    28441 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    28501 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    28561 ggttcgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    28621 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    28681 aggcgcaaca cctgcggtgt aagcaatata tttattgatg tagcgaccgt ccggcccgac
    28741 ctttttcgcg gtaccggttt taatggcaat acgatagcct ttaatcgccg ccttcacgcc
    28801 gccgccaccc ggtagcgcca cgctttccat catatgcacc acagtgcgga caatagattc
    28861 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    28921 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggag ttaccattag
    28981 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    29041 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    29101 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    29161 actcgacttc tgtaatactc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    29221 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    29281 acgttgcaac gcggtcatca ccaccatcgg tttaaccgtt gaacccggtt caaacacgtc
    29341 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    29401 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    29461 accggactca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    29521 caggcgttca tcaatactca gcgccaggtt gtgcgcagcc tggctgtcag tagaagaaat
    29581 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    29641 aagccattta tcgaaactct tctcaacgcc ctcaatacct tgactatcga cgttagtaaa
    29701 gccgatgagg tgagcagtca cttcgccgga tggataataa cggcgagatt cttcacgcag
    29761 atgaattccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt ttacctgacg
    29821 cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag
    29881 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    29941 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    30001 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    30061 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    30121 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    30181 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    30241 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    30301 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    30361 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    30421 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa
    30481 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    30541 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    30601 ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac tgaactacgg
    30661 gcacgaggat tctcagccac ctcttcttcg cccggcatta atttgcctag tgctcgcagc
    30721 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    30781 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    30841 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    30901 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    30961 ggatgtttaa acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg
    31021 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg ctcgggcaat gcgtttggca
    31081 aaacgctctt caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt
    31141 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    31201 cgcataaagg aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac accaagatcg
    31261 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    31321 gaaaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    31381 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    31441 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    31501 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    31561 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    31621 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    31681 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    31741 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    31801 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    31861 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    31921 ttgaccggca gcgttctcaa gcagctgttc ccgataacgg gtaggcactg ataagcgccc
    31981 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    32041 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    32101 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    32161 ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa gaaaaaaacc
    32221 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    32281 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    32341 ctgtgtagcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    32401 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    32461 tattgtgaat ttcagctggt gagttaatta actggcgggt ttaaaagcat ttatcaataa
    32521 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccagtcccgt actctacgcg
    32581 ccagagtgaa attcacctgg cgcgtatttt tgttcgcagc ttagctacgg ctgagcacac
    32641 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
    32701 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
    32761 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
    32821 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
    32881 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
    32941 ataactgggc agccgcctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
    33001 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
    33061 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
    33121 tttcggcatc atcctgatcg gcaccaacca cgctggtaaa gtgttcacga tcgagggcgc
    33181 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
    33241 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatgc
    33301 agcgcatttc gttgtctggc tgatcttctg agcaggcaat cagcagttga taaccccgtt
    33361 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
    33421 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
    33481 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcactcacac
    33541 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
    33601 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
    33661 attgtactgg tatgggttaa attcattaac atctaagcgc agaaaattaa ctgcggcaac
    33721 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
    33781 gtaaattgcg cattgagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
    33841 cacaccagag cgagcaactt ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc
    33901 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcaat
    33961 aatttgccca cggaatattt ccgtattacg tttaacttcg tcacgcccgt aaccgctggc
    34021 ctgaattttc accagcatga tttccctctc aacatgcgcg ccctgcccca actcactcac
    34081 gcgcaagaca tcgaccagct tgtataattg cttttcgatc tgctcaagca ctttttcatc
    34141 gcccacggtc tggatggtca tacgcgataa tgtcggatcg tcggttggcg caacggtcag
    34201 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
    34261 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
    34321 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcactgcca
    34381 tcgacggtga catcaacaaa taccaggcga ttattacgca cctgttcaag cgcctcgcta
    34441 agtttgcttt ccagctcttg cggatgagag atctggatcc cgacgtgacc ataggcttcc
    34501 gccagacgga cgaagtctgg taaagactgc atataagatt gtgaatgacg accggaatag
    34561 atcatgtcct gccactgctt caccatcccc aaatagcggt tattaagatt aaccaccagc
    34621 accggcagtt catattgcaa cgctgtagac agttcctgaa tgttcatctg aatactgccg
    34681 tcgccagtga cgcaaaccac ggtttcttct ggcagcgcca ttttgacgcc cagtgccgca
    34741 ggtaaaccaa agcccatagt gccgaggcca ccggagttga tccaacgacg gggtttgtcg
    34801 aatggataat aaagcgcagc aaacatctgg tgctgcccga catcggacgt aacgtaagcg
    34861 ccccccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
    34921 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatctgctg ccaccagtcg
    34981 cggatctcat ccagcggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
    35041 aggacctggc gagcatcccc cacaatcgga atatccgcag tcacggtttt agaaatggaa
    35101 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgtta
    35161 gtggtgcgat cgtcaaagcg gacaccgaca gcaaaaatca catccgcgtt atgcatcgtc
    35221 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
    35281 ggaaacgctc ccagccccat caatgaggaa acaacgggca gatttaacgc ctccaccgtt
    35341 tctttcagct gttgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
    35401 ttttttgccg ccaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
    35461 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
    35521 ggattaagaa tatctttcgg taaatcaacg accactggtc ctgggcgacc acttgccgcc
    35581 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
    35641 tgtttaacca ccggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
    35701 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
    35761 gtggcgatgc cggtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
    35821 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
    35881 actaaaacat gaccgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
    35941 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
    36001 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
    36061 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgccgggtaa
    36121 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggataa
    36181 aaatgataat aaatcggcta aatcccacca cttacagcaa tttctctatt tttttaatta
    36241 atcctaaaat agagagccca cacattcaga ataaaattct acactcaata aaaaatgcag
    36301 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
    36361 ctaattgctc ttccatccac tgatggcctt tatcacgccc ggcagcttca tgccaggaga
    36421 gataacaggt tctgctattt agttttaacg gcagcggtaa tacctgtaat tctaaggatt
    36481 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
    36541 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
    36601 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
    36661 cgttataaac atcatgtttc agtaacgggc ccttaattgt tgggtgattt ttgctggcta
    36721 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
    36781 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
    36841 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
    36901 gcgaggtcag aatactgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
    36961 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
    37021 ctgaaccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
    37081 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
    37141 acattcccag aacatgagca gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
    37201 cggttaataa gttgagatcg accatgcgta gctgtggctt gctgaactcc gccgtctctg
    37261 gatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
    37321 ttattggaat gcagagaaat aatcctcgag aatatgattt atcatgcata taaaataaga
    37381 aaaaggaaaa tgagtaaaat taggaaaaca taaagatacg ggttcttata aaaacagaat
    37441 ccattgccgt ggaaagcgtc atttaccaca gcataataat ccataatgtt cttgttgcgt
    37501 aaacaatcta atcataacta c
//
LOCUS       DAZFTN010000041        37412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFTN010000041 DAZFTN010000000
VERSION     DAZFTN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1084
                     /locus_tag="ACHKV2_002204"
     CDS             <1..1084
                     /locus_tag="ACHKV2_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6940085.1"
                     /translation="LIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTS
                     LALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNH
                     TATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYN
                     YTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESL
                     LSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGE
                     VFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNIS
                     LVMTEADKALREAKSEGGNKVIIHHI"
     gene            1265..2755
                     /gene="dgcJ"
                     /locus_tag="ACHKV2_002205"
     CDS             1265..2755
                     /gene="dgcJ"
                     /locus_tag="ACHKV2_002205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO6940086.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2798..3301
                     /gene="yeaK"
                     /locus_tag="ACHKV2_002206"
     CDS             2798..3301
                     /gene="yeaK"
                     /locus_tag="ACHKV2_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO6940087.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3302..3406)
                     /gene="yoaI"
                     /locus_tag="ACHKV2_002207"
     CDS             complement(3302..3406)
                     /gene="yoaI"
                     /locus_tag="ACHKV2_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO6940088.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3576..4022
                     /gene="yeaL"
                     /locus_tag="ACHKV2_002208"
     CDS             3576..4022
                     /gene="yeaL"
                     /locus_tag="ACHKV2_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO6940089.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(3979..4800)
                     /gene="nimR"
                     /locus_tag="ACHKV2_002209"
     CDS             complement(3979..4800)
                     /gene="nimR"
                     /locus_tag="ACHKV2_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO6940090.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            4897..6078
                     /gene="nimT"
                     /locus_tag="ACHKV2_002210"
     CDS             4897..6078
                     /gene="nimT"
                     /locus_tag="ACHKV2_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO6940091.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6133..6480
                     /gene="yeaO"
                     /locus_tag="ACHKV2_002211"
     CDS             6133..6480
                     /gene="yeaO"
                     /locus_tag="ACHKV2_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO6940092.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6502..6756)
                     /gene="yoaF"
                     /locus_tag="ACHKV2_002212"
     CDS             complement(6502..6756)
                     /gene="yoaF"
                     /locus_tag="ACHKV2_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO6940093.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            6939..7964
                     /gene="dgcP"
                     /locus_tag="ACHKV2_002213"
                     /pseudo
     CDS             6939..7964
                     /gene="dgcP"
                     /locus_tag="ACHKV2_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            7997..8095
                     /gene="yoaK"
                     /locus_tag="ACHKV2_002214"
     CDS             7997..8095
                     /gene="yoaK"
                     /locus_tag="ACHKV2_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO6940094.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8098..8172
                     /gene="yoaJ"
                     /locus_tag="ACHKV2_002215"
     CDS             8098..8172
                     /gene="yoaJ"
                     /locus_tag="ACHKV2_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO6940095.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8231..8479)
                     /gene="yeaQ"
                     /locus_tag="ACHKV2_002216"
     CDS             complement(8231..8479)
                     /gene="yeaQ"
                     /locus_tag="ACHKV2_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6940096.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8626..8808)
                     /gene="yoaG"
                     /locus_tag="ACHKV2_002217"
     CDS             complement(8626..8808)
                     /gene="yoaG"
                     /locus_tag="ACHKV2_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO6940097.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8812..9171)
                     /gene="yeaR"
                     /locus_tag="ACHKV2_002218"
     CDS             complement(8812..9171)
                     /gene="yeaR"
                     /locus_tag="ACHKV2_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO6940098.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9344..9982)
                     /gene="leuE"
                     /locus_tag="ACHKV2_002219"
     CDS             complement(9344..9982)
                     /gene="leuE"
                     /locus_tag="ACHKV2_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO6940099.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10109..11032)
                     /gene="dmlR"
                     /locus_tag="ACHKV2_002220"
     CDS             complement(10109..11032)
                     /gene="dmlR"
                     /locus_tag="ACHKV2_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO6940100.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11135..12220
                     /gene="dmlA"
                     /locus_tag="ACHKV2_002221"
     CDS             11135..12220
                     /gene="dmlA"
                     /locus_tag="ACHKV2_002221"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO6940101.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12471..14081
                     /gene="yeaV"
                     /locus_tag="ACHKV2_002222"
     CDS             12471..14081
                     /gene="yeaV"
                     /locus_tag="ACHKV2_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO6940102.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14113..15237
                     /gene="yeaW"
                     /locus_tag="ACHKV2_002223"
     CDS             14113..15237
                     /gene="yeaW"
                     /locus_tag="ACHKV2_002223"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO6940103.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15293..16258
                     /gene="yeaX"
                     /locus_tag="ACHKV2_002224"
     CDS             15293..16258
                     /gene="yeaX"
                     /locus_tag="ACHKV2_002224"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO6940104.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHQSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16312..17427)
                     /gene="rnd"
                     /locus_tag="ACHKV2_002225"
     CDS             complement(16312..17427)
                     /gene="rnd"
                     /locus_tag="ACHKV2_002225"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO6940105.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17509..19194)
                     /gene="fadD"
                     /locus_tag="ACHKV2_002226"
     CDS             complement(17509..19194)
                     /gene="fadD"
                     /locus_tag="ACHKV2_002226"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO6940106.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19399..19980)
                     /gene="yeaY"
                     /locus_tag="ACHKV2_002227"
     CDS             complement(19399..19980)
                     /gene="yeaY"
                     /locus_tag="ACHKV2_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO6940107.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20020..20715)
                     /gene="tsaB"
                     /locus_tag="ACHKV2_002228"
     CDS             complement(20020..20715)
                     /gene="tsaB"
                     /locus_tag="ACHKV2_002228"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO6940108.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20773..22683)
                     /gene="yoaA"
                     /locus_tag="ACHKV2_002229"
     CDS             complement(20773..22683)
                     /gene="yoaA"
                     /locus_tag="ACHKV2_002229"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO6940109.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22815..23159
                     /gene="yoaB"
                     /locus_tag="ACHKV2_002230"
     CDS             22815..23159
                     /gene="yoaB"
                     /locus_tag="ACHKV2_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6940110.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            23581..23880
                     /gene="yoaC"
                     /locus_tag="ACHKV2_002231"
     CDS             23581..23880
                     /gene="yoaC"
                     /locus_tag="ACHKV2_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO6940111.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(24000..24179)
                     /gene="yoaH"
                     /locus_tag="ACHKV2_002232"
     CDS             complement(24000..24179)
                     /gene="yoaH"
                     /locus_tag="ACHKV2_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO6940112.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            24253..25614
                     /gene="pabB"
                     /locus_tag="ACHKV2_002233"
     CDS             24253..25614
                     /gene="pabB"
                     /locus_tag="ACHKV2_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO6940113.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            25618..26196
                     /gene="nudL"
                     /locus_tag="ACHKV2_002234"
     CDS             25618..26196
                     /gene="nudL"
                     /locus_tag="ACHKV2_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO6940114.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            26380..27744
                     /gene="sdaA"
                     /locus_tag="ACHKV2_002235"
     CDS             26380..27744
                     /gene="sdaA"
                     /locus_tag="ACHKV2_002235"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6940115.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            27875..29473
                     /gene="pdeD"
                     /locus_tag="ACHKV2_002236"
     CDS             27875..29473
                     /gene="pdeD"
                     /locus_tag="ACHKV2_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6940116.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(29477..31033)
                     /gene="yoaE"
                     /locus_tag="ACHKV2_002237"
     CDS             complement(29477..31033)
                     /gene="yoaE"
                     /locus_tag="ACHKV2_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO6940117.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(31021..31179)
                     /gene="yoaL"
                     /locus_tag="ACHKV2_002238"
     CDS             complement(31021..31179)
                     /gene="yoaL"
                     /locus_tag="ACHKV2_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO6940118.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            31496..32467
                     /gene="manX"
                     /locus_tag="ACHKV2_002239"
     CDS             31496..32467
                     /gene="manX"
                     /locus_tag="ACHKV2_002239"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO6940119.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            32530..33330
                     /gene="manY"
                     /locus_tag="ACHKV2_002240"
     CDS             32530..33330
                     /gene="manY"
                     /locus_tag="ACHKV2_002240"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO6940120.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            33343..34194
                     /gene="manZ"
                     /locus_tag="ACHKV2_002241"
     CDS             33343..34194
                     /gene="manZ"
                     /locus_tag="ACHKV2_002241"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO6940121.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            34249..34707
                     /gene="yobD"
                     /locus_tag="ACHKV2_002242"
     CDS             34249..34707
                     /gene="yobD"
                     /locus_tag="ACHKV2_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO6940122.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
     gene            35018..35140
                     /locus_tag="ACHKV2_002243"
     CDS             35018..35140
                     /locus_tag="ACHKV2_002243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940123.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            35137..35703
                     /gene="mntP"
                     /locus_tag="ACHKV2_002244"
     CDS             35137..35703
                     /gene="mntP"
                     /locus_tag="ACHKV2_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO6940124.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(35700..36509)
                     /gene="rlmA"
                     /locus_tag="ACHKV2_002245"
     CDS             complement(35700..36509)
                     /gene="rlmA"
                     /locus_tag="ACHKV2_002245"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO6940125.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(36675..36884)
                     /gene="cspE"
                     /locus_tag="ACHKV2_002246"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(36675..36884)
                     /gene="cspE"
                     /locus_tag="ACHKV2_002246"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6940126.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(36897..37040)
                     /gene="yobF"
                     /locus_tag="ACHKV2_002247"
     CDS             complement(36897..37040)
                     /gene="yobF"
                     /locus_tag="ACHKV2_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO6940127.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         9220 a         9456 c         9140 g         9596 t
ORIGIN      
        1 ccttatctat ttagttgaaa ccattgtaat tatccataaa ccaattaacg gcagtacact
       61 gatccagaca aagtcgaatg atgtttctat tttctatatt ttccgccaac tcagttttat
      121 ttgtttaacc tcgctggcgc tcttttgtta tggaaaagac aacatccttg acaacaataa
      181 gaaaaaaact ggcattctgt tgctggcgct gatccctttt ttagtttttc cccttctggc
      241 acacaatctg agcagttata acgctgacta ttctttgtat gtcgtcgatt actgtccgga
      301 caatcatact gcgacctggg gaatcaacta tacaaaaata ttggtttgtc tgtgggcatt
      361 tttactgttc tttattatca tgcgtacacg attagccagc gaactatggc cgttaatagc
      421 attattatgt ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa
      481 ttataccatc tggtatatca gtcgcgggat tgaagtctcc agtaaactgt ttgttgtgtc
      541 ttttctgatt tataacattt ttcaggagct gcaactctcc agcaaactgg cagttcatga
      601 tgtgctgacc aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc
      661 gcgacctgtt gttaaggact tctgtgtcat gctggttgat attaatcagt tcaaacgcat
      721 caatgcccaa tggggacatc gtgtgggtga taaagtgctg gtttcaattg tcgatattat
      781 ccagcaaagc atccgccccg atgatatttt agcgcgactg gagggtgagg tgtttggctt
      841 gctatttacc gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa
      901 tgtcgaactc ctgaccggct ttagtaacag atatgatgtt cctgaacaaa tgaccatcag
      961 tattggcaca gttttttcaa cgggtgacac gcgtaatatc tcgcttgtca tgacggaagc
     1021 agataaagcc ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcatcatat
     1081 ttaagtgcaa aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac
     1141 tattacccct aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt
     1201 gccaggccgc aatcgaactt tatctggttt tctcgtttca ctaaccgaag gagtgccatt
     1261 tatcatgaaa ttgcaccata gaatgctccg gcattttatc gccgcaagtg tcattgtgct
     1321 gacatcttcc ttccttattt ttgaacttgt cgccagcgac agagcaatga gtgcctatct
     1381 gcgctatatc gtgcagaaag cagactcctc ctttctttat gataagtatc agaatcagag
     1441 tattgccgcg catgtgatgc gcgctctcgc tgctgagcag tcggaagtgt cgccagaaca
     1501 gcggcgcgcc atctgcgagg cttttgagtc tgccaataat acccatggct taaacctgac
     1561 tgcccataaa tacccgggct tacgcggcac attacaaacc gcatccactg actgcgacac
     1621 aattgtggaa gctgcagcac tattacccgc ttttgatcag gcagtggaag gcaaccgcca
     1681 ccaggatgat tacggttcag gtcttgggat ggccgaagag aaatttcact attatctcga
     1741 tctcaatgac cgctatgtct atttttatga gccggttaat gttgaatact ttgcgatgaa
     1801 taactggtcc atcctgcagt caggaagtat tggcatcgat cgcaaagata ttgaaaaggt
     1861 atttaccggg cgtaccgtat tgtcgagcat ttaccaggat cagcgtacta aacagaacgt
     1921 gatgagtttg ctgacgccgg tgtatgttgc agggcagcta aaagggattg tgctgctgga
     1981 tattaacaaa aacaatctgc ggaatatctt ttacactcat gaccgccctc tcctctggcg
     2041 ttttctcaat gtcacgctaa ccgatacaga ttcggggcgc gacattatca tcaaccagag
     2101 cgaagataat ctgttccagt atgtcagtta cgtccatgac ttaccgggcg gcattcgtgt
     2161 ctcgttatcc attgatattc tttactttat cacgtcttcg tggaaaagcg ttctgttctg
     2221 gattttgacg gcgttaattt tgctgaatat ggtgcggatg cacttccgct tataccaaaa
     2281 tgtgtcgcga gaaaatatta gtgatgcgat gactggactg tataatcgca aaattttaac
     2341 ccctgaactg gagcagcggt tgcagaaact ggtgcaatcc ggttcttcgg tgatgtttat
     2401 tgcaattgac atggacaagt taaagctaat aaatgacacc ctcggtcatc aggaagggga
     2461 tttagcgatt acgttgttag ctcaggcgat taaacaatcg attcgtaaaa gtgattatgc
     2521 catccgactc ggtggcgatg aattctgcat cattcttgtc gattcgacgc cgcaaattgc
     2581 agcacaactg cctgaacgta tcgaaaaacg tctgcaacat atcgcgccgc agaaagagat
     2641 cggcttctct tccggtattt acgcgatgaa agaaaacgat acgttacatg atgcgtataa
     2701 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     2761 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     2821 cgtgacgcat cagcggttaa tcgctttatt atcacaagaa ggtgctgatt tccgcgtcgt
     2881 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggca ccgctctagg
     2941 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     3001 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     3061 attacgcgct tcgttggcca gcccggcgga agtcgatgaa ctcacaggct gtgtcttcgg
     3121 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     3181 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     3241 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     3301 actagcctgt ccgttcaata agcagaacgg acaaaaccag cacaacagca atggcaaaaa
     3361 acgatgacgt gataatcagt gtctcgacga acatttgatc gttcatagca tacccccggg
     3421 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     3481 tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcgtaaagt
     3541 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     3601 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     3661 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     3721 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     3781 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     3841 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     3901 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     3961 gcgtaccggt tggcccgctt attgccacgg gtctggtttc gctgattgtg gggaaacagt
     4021 agttaatccc gcaatatatc ggcctggcgt ttgccccagg cctttcttaa acatagtaat
     4081 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     4141 cactaatccc tgaagcgcca taatcagctg taactgctgt cgccactggc gaaaactcaa
     4201 ccccgtctct tttactatca gtcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     4261 ccattgcccc aatgcccccc actcgacggg ctctttcgcc atcatctcca ccatcgcgcg
     4321 gattttagga tgagaagaaa cgggtaggtg cagctgttgt tgtggctgct gagggagttc
     4381 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     4441 gtttgccaga gttaaaatta attcccggca taagggcgat attttcaatg tgtaacatgt
     4501 tgtcggcatt gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt
     4561 cacttgattg ctatgttcca ccccgcccgg tatccaaacc gcatattgtg gcggcaccat
     4621 ccacaaagca ttttccaccg tacaggtaat tgcaccatgt agcgccagta tcagctgtcc
     4681 tttgcggtgc tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc
     4741 gaccgcggct tcatggtgtc ggtcgggttc atagccattg agattcaacc tgtgcatcat
     4801 tttgtccgaa tttagcgata atttgtcatt ttagcttgat tcaacataac aataaaaacg
     4861 gtaaggtaca gcctcgtttg taacaatgag aagcatatga cctgttcaac ttcattaagc
     4921 ggcaaaaaca ggattgtcct tatcgctggc attctgatga ttgccactac attacgcgtc
     4981 accttcaccg gcgcagcacc gttactcgat acgattcgtt ccgcttactc gctgacgact
     5041 gcgcaaaccg gcttattgac caccctgcca ttattggcct ttgcgctaat ctcacctctg
     5101 gctgccccgg tggctcgacg ttttggtatg gaacgtagcc tgtttgccgc gttacttttg
     5161 atctgtgcag gtatcgcaat tcgctctctc ccttcgcctt acttattatt tggcggtaca
     5221 gcggtcattg gcggtgggat tgcattaggt aatgtcttac tgccaggatt aattaaacgc
     5281 gatttccctc attccgtcgc cagacttacc ggcgcatatt ccctgacaat gggagctgcg
     5341 gcggctctgg gatcggctat ggtagtgccg ctggctttga acggttttgg ctggcaaggc
     5401 gcgttgctca tgctgatgtg ttttcctctg ctggctcttt ttttatggct gccacagtgg
     5461 cgaagccaac aacatgcaaa tttgagtact tcacgcgcct tacatactcg gggtatctgg
     5521 cgttcgccgc ttgcctggca ggtaacattg tttcttggga tcaactcact ggtctattac
     5581 gtgattattg gctggcttcc ggcgatcctc atcagtcacg gctatagcga agcacaggcg
     5641 ggttcactgc atggtttgct gcaactagcc acagcagctc ccggtttgct gatcccactt
     5701 ttcttacatc atgtgaaaga ccagcgtggt attgcagcgt tcgttgcctt gatgtgcgca
     5761 gtgggcgcgg ttgggctctg cttcatgcca gcgcacgcga tcacctggac tctgcttttc
     5821 ggttttggtt ccggcgcaac aatgatactg gggttgacgt tcattggtct gcgggctagt
     5881 tctgcgcatc aggcggcggc actctcgggg atggcacaat ccgtcgggta tttgttggca
     5941 gcctgtgggc cgccgctgat gggtaaaata cacgatgcta acggtgactg gtccgtacca
     6001 ctaatgggtg ttgcaatact ttcactactg atggcgattt tcggactttg tgccgggaga
     6061 gacaaagaaa ttcgctaata tccggtacta tagtgacgtg acaaatcatg cgtgaaaggg
     6121 agaacaaaca cgatgaatat tcagtgcaaa cgcgtttatg atccggcaga acagagcgat
     6181 ggttatcgcg tactggtcga ccgcctctgg ccacgcggta tcaaaaaaac cgatttagcc
     6241 cttgatgagt gggataaaga aatcacgccg tcaacggaac tgcgcaaagc ctttcacggc
     6301 gaagtcgtcg attttgcaac ctttcgcgag caatatcttg cagaactggc acaacacgag
     6361 caagaaggaa agcggctggc ggacatcgcc aaaaaacagc cgctgaccct gctctactca
     6421 gcaaaaaaca ccacgcagaa ccatgcgctg gtgctggccg actggctacg tagcttgtga
     6481 ttttagtaca gcatccggcg gttatttttc accagccgga tggtccagtc gccacaatgc
     6541 ccattcatca atcgtttcac cgcccggtaa tttgcaattg ttgctgaccc cttgcgctgt
     6601 ctgcactgga atgagcgtcc cgcccttctg ctggcaatag accgacgccg gatttgccat
     6661 accaatctgc ggcggtttag gtgcttctgg ctgagaaggg gttgaacaac cagccaggac
     6721 cagcaagcaa ggcagaacaa aactgataat tttcatttat tgatctcaca tatgtatcca
     6781 agcttagagt atcgcggtat cgttttgttt tgcagcacta tttttattac attcactcaa
     6841 aacatattac gtcttgtttc atctttgttg atgatgtttt atcatgcctg caaagattaa
     6901 ataatcagca tttacccgcc gtatcctgga gttgtttcgt gtcagatcag attatcgccc
     6961 gcatctcgca atcccttgcc aaagaacagt cactggaaag tctggtccga cagcttctgg
     7021 agatgctgga aatggtcact gatatggaat caacctacct gaccaaagtg gatgtcgaag
     7081 cgcgcctgca gcatattatg tttgcccgta acagccagaa aatgcacatc ccggagaatt
     7141 ttaccgtctc gtgggattac tcgttatgca aacgcgccat tgatgaaaac tgctttttca
     7201 gcgatgaagt ccctgaccgt tggggcgact gtattgcggc acgcaatctt ggcatcacca
     7261 catttctgag cacgccaatt cacttaccgg atggatcatt ctatggcacg ctttgcgccg
     7321 ccagcagtga gaagcgccag tggagtgaac gcgcggaaca ggttttacag ttattcgccg
     7381 gactgattgc acaatatatt caaaaagagg cactggttga acagctgcgc gaagccaatg
     7441 ctgcgctgat tgcgcaatcg tataccgact cgttaaccgg gctaccgaat cggcgggcga
     7501 tttttgaaaa tctgacgacg ctgttttccc tcgcccggca tcttaaccaa aagataatga
     7561 tcgcgtttat cgatctggat aacttcaaat taatcaatga tcgttttggt cataatagtg
     7621 gcgatctgtt tctcattcag gttggcgagc gccttaatac gctccagcaa aatggcgaag
     7681 ttattggtcg tctcggcggt gatgagtttt tggttgtttc actaaacaac gagaatgcgg
     7741 atatttcgtc gctgcgagaa cgcattcagc agcaaatacg cggataatat cacttaggtg
     7801 atgttgattt gtattatcct ggtgccagtc ttggcatagt agaagtcgat cctgaaacga
     7861 ccgatgcaga cagtgcccta catgctgccg atatcgcgat gtatcaggag aaaaaacaca
     7921 aacagaaaac accttttgtc gcgcattcag cgccacattc ctgaggcgta ttcacatcct
     7981 tttgattggt gataacatgc gaatcggtat tatttttccg gttgtaatct tcattacagc
     8041 ggtcgtattt ttagcatggt tttttattgg cggctatgct gccccgggag cataaagatg
     8101 aaaaaaacaa cgatcattat gatgggtgtg gcgattattg tcgtactcgg cactgagctg
     8161 ggatggtggt aacgtcacat ctaaaaaata gcaaaggctg cctgtgtgca gcctttgtgc
     8221 aatttacgcg ttaactttta atcttcctgt agataaatag cacgacaatc gcaccaataa
     8281 cggcaaccac gaagctgcca aaattgaagc catcgacttt accaaagcca aacagcgtgc
     8341 tgatccaacc gccgactacg gcaccgacta tccccagcag gatagtcata aagaatccac
     8401 caccatcttt acctggcatg atccacttcg ccagaatacc ggcaataagc ccaaaaataa
     8461 tccatgacag aatgcccatt gtttcctcac ttatctgttt tgcattagcg ggttagtcgc
     8521 tgataaaaag catagcacaa catcgggagg gcaagatttg tgacgagcat cacggaggtt
     8581 tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc aaccacaaac
     8641 atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt ttatcacttc
     8701 agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat cgacagaaac
     8761 gccattgctg ttattggtga cggtcacggt atacgttgct ttgcccatga ttcatttccc
     8821 gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag ccacgaagaa
     8881 gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact tttctggagg
     8941 gaacaccgca aactgccccg cttcgataag gatcacctga tcaggctctg cactgtgttc
     9001 atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg ggtaaacccc
     9061 cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag tttgtttatt
     9121 ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca tccttttccc
     9181 tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac gcctgataat
     9241 ttgcttttta aataccattt attggttact ttttagcacc atatcagaga aaaatcaggg
     9301 aggattatag atgggaagcc cgcgacaacg cgggctgaaa gcatcaggat tgcagcgtcg
     9361 ccagtcgggc agcgaaaccc acgaacatca aaccaatcag tgagttgccc actttagcca
     9421 gtttcttttt ggtacgtatg tactgcgtga caaaagcacc agatataatc aggaagctca
     9481 aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa attcccgtat
     9541 gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa atggctttcg
     9601 gattagtcag gctcaaaatt aacgcgcgtt taaaaatagc accgtattgg ggctcatcgg
     9661 atttggcctc gttattttta cccttcaggg tcgcgtaaag aattttactc cccagataga
     9721 gcaaataaaa cgcaccaaga taacgtacaa tgttgaataa tatcggggtg gtcttaatta
     9781 atgtcgccat tccagcccat gccagaaaca tcaataccgc atcgccaata aatacaccgc
     9841 aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt acaaacaggg
     9901 tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc cagtaattca
     9961 gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca tgcgcgatta
    10021 aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag gttttaacct
    10081 gatatcaacc cgataattga atcattaatc aggcatgctc cagtgaaaaa ttcgggtagt
    10141 gctcggcaaa atactggcgt aaaaattcta ccgttatccg cactttcgct gacgtcgcca
    10201 gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt agcacttgca
    10261 ctaaatgacc actggcaatg ttttcgctaa catcccacca ggaacgcaga gcaatcccct
    10321 gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt gggccggtga
    10381 ctttaatcgc atggtggcct tctttattgc gtaattgcca gacaccaaaa ggatgatcgc
    10441 gctctttaat gaccaggcac ggcaatgcag aaagatcggt taaatgtttc ggcgcgccat
    10501 gttgcgcaat aaattccggc gacgcgcaga gaatgcggta attggtcgcc agtttgcggg
    10561 cgattaaatt aggggcgatg tcatcgccaa tgcgaatatc gagatcgaca ccttcattga
    10621 ccaaatcgac cagccggtct tccacatcaa aacgtaattc cagttgcgga tacgccttcg
    10681 ccagcgctga taacgccgga gccaccacct gtcgtccaaa accaaagctg ctgataatac
    10741 gcagcatccc ctgtggcacc tgacgcacgt cagaaagttc gtccatcatc tgaccgacat
    10801 cctgcaaaat ccgctgcgcc cattcataaa tccgctctcc ttcttcggta atggtgacgc
    10861 ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag gcgatgcgct
    10921 tgctgacgaa cgccggtgaa acgcccagtt cttccgccac ggcggcaaaa ccagcccggc
    10981 gagccaccag cataaagacg cgcaaatcat tcagcagcgg taaattattc atgattcgtg
    11041 ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag gatggtgatg
    11101 ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc gtattgctgc
    11161 gatcccggga gacgggattg gcaaagaagt ccttcctgaa gggattcgcg tgttacaggc
    11221 tgccgctgag cgctggggct tcgccttgag ttttgagcaa atggagtggg cgagctgcga
    11281 gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac ttagccgttt
    11341 cgacgccatc tattttggtg ccgtcggctg gccggacacc gttccggacc atatttcgtt
    11401 gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc tgcgcccggt
    11461 tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg acatcgattt
    11521 ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggta gagtgaatga
    11581 aggtacagag catgaagtcg tcattcagga atcggtattt acgcgtcgtg gtgtcgatcg
    11641 cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc taacttctgc
    11701 cactaaatca aacggtttag ccatcagcat gccgtactgg gatgagcgag tggaagcaat
    11761 ggccgagaat tacccggaga tccgctggga caagcagcat attgatattc tctgcgcgcg
    11821 ttttgtgatg cagccggaac gcttcgatgt ggtggtggcg tccaatttgt ttggcgatat
    11881 cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat ccgccaacct
    11941 gaatccggaa cgcactttcc cctcgctctt cgagcctgtc cacggttccg cgccggatat
    12001 ctacgggaaa aatattgcta accctatcgc cacaatttgg gccggggcaa tgatgctcga
    12061 ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc tggcagcgat
    12121 tgaagaagtg attgctcacg ggccgaaaac accggatatg aaaggcagtg ccaccacgcc
    12181 acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca gtttatttga
    12241 accgcgtcac tgacgcggtt ttgttattcg ttctttgcag ttaataacct gcgtcatttc
    12301 accttttatt gtttccgttt cgtgttttat ggctttccgt attcttaatt gtttaattta
    12361 tgtaacatgc aaataatcaa ttcgttaaac acgacataca actcattacc ctgcaaataa
    12421 ttacaactgg caaaagccag agacgggctt ttatgcccag gagatgactc atgatgagca
    12481 atgttaagaa aaaagatgtg ccgctgataa gcatcagcct ggtggccatt cttttcatcg
    12541 cagctgcatt aagccttttc ccacaacaat cggccgacgc ggccaacgca atatacactt
    12601 ttgttacgcg tacgttaggt tccgcagtac aggtattggt tttgctggca atgggactag
    12661 tgatttattt agccaccagt aaatacggca atattcgtct tggcgaagga aaaccggaat
    12721 acagcacgct ctcctggctg tttatgttta tttgtgccgg tttaggttct tctacgcttt
    12781 attggggggt tgctgaatgg gcctattatt atcaaacgcc tggattaaat atcgcaccgc
    12841 gttcacaaca ggcactcgaa tttagcgtcc cttactcctt cttccactgg ggcatcagcg
    12901 cctgggcaac ttatacgctg gcctcattaa tcatggctta tcactttcat gtgcggaaaa
    12961 acaaaggtct gagtctttcc ggcattattg ccgcgattac cggcgttcgc ccgcaaggcc
    13021 catggggaaa actggttgat ttgatgttcc tgatcgccac tgtcggcgca ctgactattt
    13081 cccttgttgt caccgcagca acctttactc gtggactttc cgcactgacc ggtttacccg
    13141 ataacttcac cgtgcaggca tttgtgatcc tgctttccgg cggcattttt tgcctaagct
    13201 catggattgg tatcaacaac ggtttgcaac gtctgagcaa aatggttggc tggggcgcgt
    13261 tcctgctgcc attactggtg ctgattgtcg gtccaaccga atttattacc aacagcatca
    13321 tcaatgccat cggcctgacc acgcaaaact tcctgcaaat gagcttattc accgatccgc
    13381 ttggcgatgg ttcatttacc cgcaactgga ccgttttcta ctggctgtgg tggatctcat
    13441 acacccctgg cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag attaaagaag
    13501 ttatctgggg actgatcctc ggcagcaccg tcggttgctg gttcttcttc ggcgtaatgg
    13561 aaagctatgc cattcatcag tttatcaatg gcgtaatcaa cgtcccacag gtgctggaaa
    13621 ctctgggcgg cgaaacagct gtacagcaag ttctgatgtc gttgccagcc ggtaaattgt
    13681 tcctcgccgc atacctgggc gtgatgatta ttttccttgc ctcgcatatg gatgcagtgg
    13741 cctacaccat ggcggcgacc agtacgcgta atctccagga aggtgacgat cctgaccgtg
    13801 ggctgcgtct tttctggtgc gtggtgatca ctctgatccc gctttccatc ttgtttaccg
    13861 gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc ttcctcgtca
    13921 ttttactggt gaaagtcggc ggatttattc gctggctgaa acaggattac gccgacattc
    13981 cggctcatca agttgaacat tatctcccgc agacaccggt tgaagccctg gaaaaaacac
    14041 cagtgctccc tgcgggaacc gtattcaaag gcgacaactg agcgccatca tcctaacgat
    14101 aaaggtatcc ctatgagcaa tctgagccct gactttgtac tacccgaaaa tttttgcgct
    14161 aacccgcaag aggcgtggac cattcctgcc cgtttttata ccgatcagaa cgcctttgaa
    14221 cacgaaaaag agaacgtctt cgccaaaagc tggatttgcg tcgctcacag cagcgaactg
    14281 gcgaatgcca atgattatgt gacgcgtgag atcattggcg aaagcatcgt gctggtacgc
    14341 ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg tcatcagttg
    14401 ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc catatcacgc atgggcattc
    14461 aaactcgatg gcaatctggc ccatgcacgt aactgcgaaa acgtcgccaa tttcgatagc
    14521 gacaaagcgc aactggttcc ggtgcgtctg gaagaatatg ccggattcgt cttcatcaac
    14581 atggacccca acgccaccag cgtagaagat cagttacccg gtctgggcgc gaaagtgctg
    14641 gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc gctttaccac ccgcacgcct
    14701 gccaactgga agaacattgt cgataactat ctcgagtgct atcactgtgg tccggcgcat
    14761 ccaggtttct ccgactccgt acaggttgat cgttactggc acaccatgca cggtaactgg
    14821 acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga agagggtacg
    14881 gatgcggcat tccacggttt ctggctgtgg ccgtgcacga tgctgaatgt caccccgatc
    14941 aaagggatga tgacggtcat ttatgaattc ccggtggatt ctgaaactac cctgcaaaac
    15001 tacgatattt acttcaccaa tgaagagtta accgacgagc aaaaatcgct gattgagtgg
    15061 tatcgcgatg tgttccgtcc ggaagattta cgtctggttg aaagcgtaca gaaagggctg
    15121 aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg acagtagcgg tagcggcatt
    15181 tccgaacatg gtatcgccca tttccataat ctgctggcgc aggtatttaa ggactaatga
    15241 catcggcggc ggtattttcc gccgccgggc tgatttttga tggagtacag caatgtcaga
    15301 ctatcaaatg tttgaagtac aggtgagtca ggtggaaccg cttaccgaac aggtgaaacg
    15361 cttcacgccg gtggcaaccg atggcaaacc gctgcctgcg ttcaccggag gaagtcacat
    15421 catagtgcag atgagcgatg gtgataacca gtacagtaat gcgtattcac tactgagttc
    15481 accgcataac acctctagtt atcagattgc cgttcgactg gaagaaaact cgcgcggcgg
    15541 ttcccgcttt ttgcatcagc aggtaaaagt gggcgatcgg ttaacgatct caacgcctaa
    15601 taacctgttt gcgctaattc cctcagccag aaagcatctg tttatcgcgg gcggtattgg
    15661 tatcacccct ttcctgtcgc acatggcaga gttgcaacac agcgacgtcg actggcagct
    15721 acattactgc tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag tccagcatcc
    15781 gcaggctgag aaagtccatt tgcatcaatc atcgaccgga acacgactgg aattagcgcg
    15841 attattggcg gatatcgaac ctggcacaca cgtttatacc tgtggccccg aggcgctaat
    15901 tgaagcggta agaagtgaag ctgcgcgtct ggacatcgct gccgatacgc tgcactttga
    15961 gcaatttgct atcgaagaca aaaccggcga tgcatttacc ctggtgcttg cccgttccgg
    16021 aaaagagttt gtggtgccgg aagagatgac tattttgcag gttattgaaa ataataaagc
    16081 cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa cggcaatact
    16141 ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg ccagccagca
    16201 aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg atttgtagtt
    16261 tgccgaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaaaat tttactgcgg
    16321 atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc agccggaaat
    16381 cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca gttggttgat
    16441 ttgccgacgc gatgccagca attcggcgct gatcttgtgc gtttcgctca cgtcagtaat
    16501 cagcgactta atcgctttaa acgctttacg ataacccggc atgtccatca ggttaagcat
    16561 cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg ccagcagcgt
    16621 tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt cgcctaaact
    16681 tcccggcata taacgcgcta ccgaccacaa atgctcttca cgcacgacaa agttcaccgc
    16741 cagatcgcgc tctcgcgcct tgcgcagtcg ccagtcggct aacagttgca gacaggccag
    16801 ttggcgtgtg cgtaattgcc aggcattggt gatatcacgc caggcatctt ccggcgcaac
    16861 gacttcctga cgacgcattt gcatcaggcg acattcatcc agcgccgctg gtagccagcc
    16921 ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc agacatccgc
    16981 cgctgcatat tcacactgac gttcggtcag cggtctggcc agccagtcgg tgcgcgattc
    17041 actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaaac cccatgacat
    17101 cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc aggggttgtg gtaattcgcc
    17161 aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt ttgtgatgga
    17221 cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag gatcaattag
    17281 cgccagatgc tcgccatcga aaagttgaat caaccccagc tgcgggtaat aagtgcgcgt
    17341 acgaacaaat tcagtatcca gggctatcgc cggaaaggca cggacggctt cacacaaaga
    17401 agccagcgca tcgtccgtgg taatcatttg gtaattcaaa ttgttttctc tttagtgggc
    17461 gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc aggctttatt
    17521 gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc cgacgttaga
    17581 tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg tgagctgacg
    17641 gcggcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca ctacgaagat
    17701 tttcaccgct tcaccactgg agccggaagg tacaccaaca gccgcgactt cctgtacgcc
    17761 aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac cggaaaccaa
    17821 aatcatgtct tttttacgat cgacaatgcg caggaatcct tcttcatcca ttaccgcgat
    17881 gtcgccagtg tgtaaccagc cgtttttgat gatttcatcg gtagcatccg gacgctgcca
    17941 atagcccagc atcacctgtg gacctctgac gcaaagttca cctggctgac ctggcgatac
    18001 ttcattatcg tcgtcatcca ccagtttggc ttccgtcgac ggcaccggca aaccaatgct
    18061 accgctatga taatcaatat cataggggtt cacgctgacc agcggcgcac attcggtcag
    18121 gccataacct tccagcagat actgtccggt cagtttcacc cagcgttctg ccaccacttg
    18181 ctgaacaggc atcccaccgc ctgcagaaag gtgcagactg gagaaatcca gttgttgaaa
    18241 ctctttattg ttcagcaacg cgttgaacaa ggtgtttacg ccggtgatag cggtaaacgg
    18301 atatttcgcc agctctttga ccagccccgg aatatcccgc gggttagtga taagcaggtt
    18361 ctgcccaccc agttcgataa acagcaggca gttaatggtc agtgcaaaaa tgtgatacag
    18421 cggcagcgcc gtcaccacca gctctttgcc cggatgcagc agaggcccat acgtagcgtt
    18481 aacttgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt tcgccacacc
    18541 tgtggtgccg ccagtgtatt gcaggaaggc taaatcttct ggcaccagtt caggtttgac
    18601 atactgcata cggtagccgt tgtgcagggc gctacgaaat gaaatggcat ctggcagatg
    18661 atatttcggc accagacgtt tgatgtattt aacaacgaag ttaaccagtg tgcctttcgc
    18721 tgtggaaagc tgatcgccca tacgggtcag gataacgtgc tgaactgcgg ttttatcgac
    18781 cactttttcc agcgtgtgag caaagttgga cacaatgaca atcgccgatg cgccgctatc
    18841 gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta cgacgatcat
    18901 cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag gcatcatcaa
    18961 cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg caaacgcgcg
    19021 actgcgttct tccagcttgc ggaaggtcat cacctccccc atattcacaa acgcaggctg
    19081 atcggcgtag cgcgcgaccg actgctcaaa catatctacc agagattgat aacggtcagg
    19141 gttgatctcc gtcggaacgt ccgcgggata acggttaagc caaaccttct tcaattcttc
    19201 acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat gatatacatg ccgttaacat
    19261 aatattaact catcatacca gcttgataat tacccaacga aaaggttgcg aagcgcgtca
    19321 ctatttattt ttatctttac cgtaagaatg cagaaacagc ggaccagccg ctgtttcttt
    19381 ttctttaaaa acaagcaatt attcagttac aactgtttgt actctcgcgg ggccgggatt
    19441 ataccagccc catccgccgt ggccataggg ccagccacga ccgccataaa accagggatc
    19501 aatcggctga ggcggcataa tcacctgctg ggttaaatgc caacgtttgt aacctgttgc
    19561 ctgcatcacc ataaatttat agggcgtgtt gccgattttt ccgtcaaccg caccagtgat
    19621 tggcccgact accgtaacca gttgtccacg gaaatccacc gggtccagaa aaccgttcac
    19681 atcggcataa atgcgaccgc gagaaggttc tcccagcgtc ggtctggctc cgctgtccag
    19741 cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtaccg caaccacttt
    19801 gccaccaaag cgtgcctcct gaccaacgta cagctgcggc gcactcatca cccgaactaa
    19861 atcttgttgc ggcgtgggac tgctgccttt aatggcgtcc ggcacagtga cacaaccgct
    19921 cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt gaaccgccat
    19981 gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc ccggaagttt
    20041 cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg ccaccgtttt
    20101 accctcggca aacatctgac aagcaatcgg cagcatatct tctgcagcgg gcagtaaaac
    20161 ttctccgtcg cgcaaaacca gtccgctctc tttaccgaga tccggccagg cttgccagcc
    20221 tgtacctacc gtcacccatt ccccggaaag ctgttgcatt cgttcatgaa cgagttcggg
    20281 tttgagtacg gcttcggttt cttcaccgtg ccagataccg ttttcatcac gctgatattc
    20341 ggcccagtaa acttcgccca ttcgcgcgtc aatggctgac agcacgcgtg ttgcgccgtt
    20401 tttgcgccac gccccttgcg ccatcgtcat tagcgtggag acgccaatca tcggtaattc
    20461 cgcgccaagc gccagccctt gcgcgatgcc aataccaatg cgcacgcctg taaagctacc
    20521 ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg gaagttccgc tggtggtcag
    20581 gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa gctcaaaatg
    20641 agcgttgaca gtaccgtcgt tccacagggc gacagagcag gcctctgtcg cggtatcgat
    20701 agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca tcataccata
    20761 ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac gggcaatgtc
    20821 acgggtgcgt ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag gacgcatcac
    20881 cagccgattg tcacaaatta ccaaaacccc gcgatcgtcg gcgtcgcgaa tcagtcgccc
    20941 gactccctgc ttgagagtaa tcaccgcatc cggcagttgt acttcatcga acgggtcgcc
    21001 accgcgcaaa cggcaatctt ccatgcgcgc ttttaacaat ggatcatcag gcgaggtaaa
    21061 cggcaatttg tcgataatca ccaatgacaa tgtatcgcca cgcacgtcca ccccctccca
    21121 gaaactactg gttgccacaa gaagcgcgtt accggcactg acaaattgct gcaacagttg
    21181 ccctttgctg gtttccccct gcaataatac gggaagcgtc atggtagcgc ggaactgctc
    21241 ggccagatcg cgcatcatgg cgtgcgaggt acaaagcata aaacagcgac cgttgttagc
    21301 ttcgatgatc gggcgcagca tagccgccag ttgccgcgcg gaacccggtt gattggtttg
    21361 cggcaggttg cgcggcacac agagtaacgc ctggcggctg taatcaaacg ggcttggcaa
    21421 tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca gatcgtcgtt
    21481 taccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca ttaactcttt
    21541 gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg aagtgcattc
    21601 gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc gtgtgcgata
    21661 caacgtggcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg acagtttcgc
    21721 cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct gaatttgcgg
    21781 attagctaac agctcacgca ggttaccgcg ataacctggt tcaccgagtt gcagacgaaa
    21841 atcctgcgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg tgtcttttaa
    21901 ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc gactggagag
    21961 tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt cgaagatcat
    22021 gacgtccgct tccgggatca actcgccaaa tccactctct ttaaccacca tatccgccag
    22081 aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac gtgctttgac
    22141 cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt tggtgctggt
    22201 gaccagcggc cacgcctgcg aatcttccgc cacgctgacg caggtgctga tatcaccatc
    22261 gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaatattt gtaccggaag
    22321 atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt ttgagcgccc
    22381 tttcagcaac gccacgttgc ccgtgtattt caacgccttt gagaccgtcg gcaaatcgcg
    22441 gctatagagc tgatcctgca acgcttttga gccggtcgag ataatgactt tctttttcgc
    22501 ccgcagcgca ggggccaggt aagcgtaggt tttgcccgta ccggttcctg cttccaccac
    22561 cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc gctgtggttc
    22621 tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa aatcgtccgt
    22681 cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc tttcgccagt
    22741 tgaagtggtg acggcgacct cacattgtgg cagtctttgc agcacgaaaa tggaaggata
    22801 atgaggataa aatgatgact atcgttcgta tcgatgctga agcccgctgg tctgatgtag
    22861 taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac gccgatgcct
    22921 ttgagcaaac cgccaacacc ctggcgcaga ttgacgccgt gctggaaaaa cagggcagca
    22981 ataaatcgag cattctggat gccaccattt tcctggccga taaaaacgac ttcgcggcga
    23041 tgaataaagc gtgggatgcc tgggttgtcg cgggtcatgc gccggtgcgc tgcacggtac
    23101 aagcgggttt gatgaacccg aagtataaag ttgaaattaa gattgtggct gcggtgtaag
    23161 ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc ggttatacat
    23221 aaatcgacca gagaataaga tattactgtt gatgcttctg ctaattcatt atttattttt
    23281 ataatttcaa ttttatctat aaaactaact atataaataa acataaccat gccgttgtgt
    23341 tcgccctttg attagcaact ctggttttct ttcttagctg aacaatctgg ttatagcgtg
    23401 cggactgacc gttaattttc tccatcttac tggataacac accatgagct gctaccttta
    23461 aaacacctga atatcaacag gttaactcgt gccatatcgt tataatccct ttgatattag
    23521 atgcaaatta aggtcatata tagccttatc gataacatgg ttaattttaa ggataagaat
    23581 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
    23641 acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg
    23701 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
    23761 gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg
    23821 attaaaaatc ctgagtactt ttcaacatat atgcaggaag aactaaaagc actggtctga
    23881 gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
    23941 tttttctcac aatcaaataa tcatcagctt ctataacgaa atcgatacgg attagtgtgt
    24001 tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc
    24061 tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg
    24121 atccgctcga cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt
    24181 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
    24241 gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac
    24301 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
    24361 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact
    24421 ttaaccactt taggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
    24481 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat
    24541 aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc
    24601 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
    24661 ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt
    24721 cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc accgcaggaa
    24781 gatttcacgc tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa
    24841 tttcgccggg tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
    24901 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
    24961 aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg
    25021 ccagagcggt ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg
    25081 ctaccacgcc tgcccgctcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca
    25141 gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
    25201 gttgccgtag caggttcggt aaaagtacca gagctgttcg tggtggaacc cttccctgcc
    25261 gtgcatcatc tggtcagcac cataacggcg caactaccag aacagttaca cgccagcgat
    25321 ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
    25381 gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat
    25441 ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac
    25501 ggacaaatat actgctctgc gggaggtgga attgtcgccg atagccagga agaagcggaa
    25561 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
    25621 gaataccgta gcctgacgct tgatgatttt ttatcgcgct ttcaactttt gcgcccgcaa
    25681 attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga
    25741 ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa
    25801 gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg
    25861 cgcgaagctg aagaagaggt tgctataccg ccttccgccg ttgaagttat cggcgtgctg
    25921 ccgcccgtcg atagcgtaac tggctaccag gtaaccccag tggtcggcat tatcccgccc
    25981 gatctgccgt atcgcgccag tgaagatgaa gtttccgctg tgtttgaaat gccgctcgcc
    26041 caggcattac atctgggtcg ttatcaccct ttagatatct accgccgcgg cgattcacat
    26101 cgggtatggc tatcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
    26161 cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa
    26221 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
    26281 tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt
    26341 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
    26401 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
    26461 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
    26521 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
    26581 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
    26641 cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
    26701 ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg
    26761 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
    26821 ggcggtttta tcgtcgatga agaacacttt ggtcaggata ctgccaacga agtgagcgtg
    26881 ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg
    26941 ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
    27001 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa
    27061 ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgctg
    27121 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
    27181 gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac
    27241 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc
    27301 agcccggaca tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa
    27361 atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt
    27421 tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg
    27481 gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag
    27541 gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca
    27601 cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
    27661 atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg
    27721 gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa
    27781 tttatccccg ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag
    27841 gcgtttgtcg ccatacgctt acagagtggc ccgcatgcaa aaagcacaac ggatcattaa
    27901 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc
    27961 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
    28021 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga
    28081 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
    28141 ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg
    28201 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
    28261 accgcaacca cttttacgcc tgacgaccga ccgtgtcctg attaaaggca gtccggtttt
    28321 gattcaatgg acgcctgcag cgagcagtag caatgctggg gtcatggaga tgattaacat
    28381 cgacttactg acggcaatgc tgcttgagcc acaactgccg caaatcagta gcgccagcct
    28441 gacggtggac aaacggcatt tgctctatgg caatgggctg gtagattccc ttccacaacc
    28501 tgaagacaat gaaaactatc aggtttcttc gcaacgcttt tcttttacca ttaacgttaa
    28561 tggtccgggg gctccggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
    28621 actgctaagt ttactggtgg gctacatcgc ctggctggcg accgcttacc gaatgagctt
    28681 ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt
    28741 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc
    28801 gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
    28861 tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat
    28921 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
    28981 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
    29041 aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg
    29101 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct
    29161 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
    29221 tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat
    29281 tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg
    29341 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
    29401 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat
    29461 gccgttacgt taacgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
    29521 ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa
    29581 tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg
    29641 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
    29701 ctgtaccgcc ttttaccagc cagccgtcac catcagtaat gatttccggc gtttcgtcag
    29761 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc aagccttgta
    29821 ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca
    29881 gcaggttgat cggatcgagg gtttcaggga cgataatcgc cggagacgcc gaagcaatcg
    29941 ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt acgattccga
    30001 tgatttcatc cagttcaccg cgacataccg ggaacaggct gtgcggtgag gagagcagct
    30061 gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg
    30121 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt
    30181 cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct
    30241 gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc
    30301 gcagcggcaa agtcgactgg tgacgaataa agttgcgacg cgcaatctgg ttaaacactt
    30361 cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga
    30421 aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca
    30481 ccgtggggtg ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca
    30541 tcgcaatcac taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa
    30601 ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga
    30661 aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca
    30721 gttcggttgt tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc
    30781 cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa
    30841 tcagcgacag cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt
    30901 tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga
    30961 tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca
    31021 ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc
    31081 aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gtgccagaaa ggcaggcgtt
    31141 aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata
    31201 ctccatgatt gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt
    31261 taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc
    31321 gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc
    31381 gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg
    31441 attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac
    31501 cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga
    31561 aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc
    31621 cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt
    31681 gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt
    31741 cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt
    31801 aatggctcgt gatgatgacc caagctttga tgaactggtt gcgctggcag tagaaacagg
    31861 ccgtgaaggc gtgaaagcac tgaaagccaa accggttgaa aaagccgcgc cagcacccgc
    31921 tgccgcagca cccaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat
    31981 ggttattggc cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg
    32041 gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac
    32101 tgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga
    32161 tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac gtgtaatgct
    32221 gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc
    32281 tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc caagtgaata acgcggtttc
    32341 ggttgatgaa aaagatatcg aggcgttcaa aaaactgaat gcacgcggta ttgagctgga
    32401 agtccgtaag gtttccaccg atccgaaact gaaaatgatg gatctgatca gcaaaatcga
    32461 taagtaacgt attgtgttga ttatcagtca gttttcacac tttagtcata cgtaaacagg
    32521 agaagtacaa tggagattac cactcttcaa attgtgctgg tatttatcgt agcctgtatc
    32581 gcaggtatgg gatcaatcct cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc
    32641 ctggtgggta tcgttcttgg ggatatgaaa accggtatta ttatcggtgg tacgctggaa
    32701 atgatcgcgc tgggctggat gaacatcggt gctgcagttg cgcctgacgc cgctctggca
    32761 tctatcattt ctaccattct ggttatcgca ggtcatcaga gcattggtgc aggtattgca
    32821 ctggcaatcc ctctggccgc tgcgggccag gtactgacca tcatcgttcg tactattacc
    32881 gttgctttcc agcacgctgc ggataaggct gctgataacg gcaacctgac agcgatttcc
    32941 tggatccacg tttcttctct gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc
    33001 gttgcgctgt ctgtaggtac cagcgaagta cagaacatgc tgaatgcgat tccggaagtg
    33061 gtgaccaatg gtctgaatat cgccggtggc atgatcgtgg tggttggtta tgcgatggtt
    33121 atcaacatga tgcgtgctgg ctacctgatg ccgttcttct acctcggctt cgtaaccgca
    33181 gcattcacca actttaacct ggttgctctg ggtgtgattg gtactgttat ggcagtgctc
    33241 tacatccaac ttagcccgaa atacaaccgc gtagccggtg cgcctgctca ggcagctggt
    33301 aacaacgatc tcgataacga actggactaa caggtgagcg aaatggttga tacaactcaa
    33361 actaccaccg agaaaaaact cactcaaagt gatattcgtg gcgtcttcct gcgttctaac
    33421 ctcttccagg gttcatggaa cttcgaacgt atgcaggcac tgggtttctg cttctctatg
    33481 gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca agctattcgc
    33541 cgtcacctgg agttctttaa cacccagccg ttcgtggctg cgccgattct cggcgtaacc
    33601 ctggcgctgg aagaacagcg tgctaatggc gcagagatcg acgacggtgc tatcaacggt
    33661 atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg acccgatctt ctggggaacc
    33721 gtacgtccgg tatttgcagc actgggtgcc ggtatcgcga tgagcggcag cctgttaggt
    33781 ccgctgctgt tcttcatcct gtttaacctg gtgcgtctgg caacccgtta ctacggcgta
    33841 gcgtatggtt actccaaagg tatcgatatc gttaaagata tgggtggtgg cttcctgcaa
    33901 aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt ggttaacaag
    33961 tggacacatg tcaacatccc gctggttgtc tctcgcatta ctgaccagac gggcaaagaa
    34021 cacgttacta ctgtccagac tattctggac cagttaatgc caggcctggt accactgctg
    34081 ctgacctttg cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt
    34141 ggcttcttcg tcatcggtat cgctggttac gcttgcggcc tgctgggact gtaagactgt
    34201 tgtacactac cggggccttt tggccccgtt tttttatctg gaggattaat gacaatcacg
    34261 gacctggtac tgattctttt catcgccgca ctcctggcct tcgcgatcta cgatcagttc
    34321 atcatgcccc gccgtaacgg ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc
    34381 atcgatagcg ttatcttcgt cggattgatt gtcattctta tctataacaa cgtcacgaat
    34441 catggtgcgt taataacgac atggttatta agcgcactgg ctctgatggg tttttatata
    34501 ttctggatcc gcgttccgaa gatcatcttt aaacaaaaag gttttttctt cgccaatgtc
    34561 tggattgaat atagccgaat caaagcgatg aacttgtcgg aagatggcgt gttggtgatg
    34621 caattagaac agcgtcggct gttaatccgc gttcgaaata tcgacgatct ggaaagaatt
    34681 tataaacttc tcgtttcaac tcaatgagtt attaatttag ccaaagctat gtttagtgta
    34741 tttttaataa tcagacatag cttaggctat attacctctt cccttatttg ttatttatta
    34801 taacgtttca ttgatatata aatctaaatg aaaatcgtta tcaacaaagc aataaaataa
    34861 tatattccaa cagttgtttt atattctcaa aatatgttaa ggttgcgccc tcatttgggg
    34921 agtagccaat ttccagattc cggaaatgta cgtgtcaaca tactcgttgc aaaacgtggc
    34981 acgtacggac tgaagacttt cagtcaggcg agaccatatg cacatcaatc gctatgcctg
    35041 catgaacgcg aaaacccatc gcggcttgtt gttcatgtat gcatgtttgc tgggggcgat
    35101 gatgtgtttt atggataccc cggtcaggac gttgtcatga atatcactgc tactgttctt
    35161 cttgcgtttg gtatgtcgat ggatgcattt gctgcatcaa tcggtaaagg tgccaccctc
    35221 cataaaccga aattttctga agcattgcga accggcctta tttttggtgc cgtcgaaacc
    35281 ctgacgccgc tgatcggctg gggaatgggc atgttagcca gccggtttgt ccttgaatgg
    35341 aatcactgga ttgcgtttgt gctgctgata ttcctcggcg ggcgaatgat tattgagggt
    35401 tttcgtggcg cagatgatga agatgaagag ccgcgccgtc ggcacggttt ctggctactg
    35461 gtaaccaccg cgattgccac cagcctggat gccatggctg tgggtgttgg tcttgctttc
    35521 cttcaggtca acattatcgc gaccgcattg gccattggtt gtgcaacctt gattatgtcg
    35581 acattaggga tgatggttgg tcgctttatc ggctcaatta ttgggaaaaa agcggaaatt
    35641 ctcggcgggc tggtgctgat cggcatcggc gtccagatcc tctggacgca cttccacggt
    35701 taataagaac gctgccagag gtgaatatta aagtccgtct ggcagtcgaa cacttctttt
    35761 gctgccagta tttgccagac ttctggcttc gcacgccagg caaacggcgt catctgcaat
    35821 aatgcgacgg cttcatcacc gcgaagacgc atcggataac acaactcatc actcttctgt
    35881 aatgtaaaac cttccagttg ttctgcatga ggtgcatgaa gatgtacttc attgtaaatc
    35941 agccccttca gctccatcaa atgtcgcggt cccggcgtgg cagtaatgac ccagccgccg
    36001 ggcttcacta ctcgtgctaa ttcttctgct ttacacggcg cgtaaatacg tattatggcg
    36061 tccatactgg tatcggaaaa cggcaaacgg tggctggaag cgacacaaag agtgacctgc
    36121 ggatagcgtt tcgccgccgc ttttatcgct accttcgaaa catccagacc aaacgtggtg
    36181 atttcgggca acgcatcggc aaatgcgtgt gtgtaatacc cttcaccaca gccaatatcc
    36241 agcaccgccg tggccttttc attaagccgt tcccgcagtt gggcgacaat tgcatcacgc
    36301 agcggctgat aatgtccggc atctaagaat gcgcggcgtg cttgcatcat ttccgcgctg
    36361 tcgcccggat cacgagaccg tttatgctga acgggcagca gattgacata cccttctttc
    36421 gccatatcaa actgatgtcg ttggggacag atatagctgt ttttttcacg cgtaagaggc
    36481 tgatggcaaa gtggacagga aaacgacatg acaactccgg caggatacta aaggccgcaa
    36541 gtgtaacgcg aatcacgcgg cgggcaaata tccgaacgcc aggagcgaag ataaagcata
    36601 gtaaaaagcc tcgcattcga cgaggcttta tagcgagatt gaagcgtatt cacacttcag
    36661 atcagtggat tcgatcagat agctgttacg ttaacagctg ccggaccttt ctggccgtcc
    36721 tgaatttcga actcaacgtt ctgaccttca gccagagttt tgaagccatt accctggata
    36781 gcggagaagt gtacgaacac gtctttgctg ccatcagcag gagtaatgaa gccaaaacct
    36841 ttagactcgt tgaaccactt aacctgacct ttaatctttg ccatttgaaa aattccttag
    36901 attgttttct tcgcccgcag gcataacata gataaaactg acacattact gcatgaggca
    36961 ccaatataag gctcggcaga gaagcggtat tcaacgtcaa cgtgtttact caggacttct
    37021 ttactgaaaa tgccacacat aaacagaact gtacctcgtt taacccgaaa tctgttatca
    37081 catacaacgt taattatggc aagccatttt taaacatgtc tcgatcagac gcacaaatct
    37141 cgatacgtct tccacttttt ttgcacactt atgcaacgga atacgcgccg atatatcatt
    37201 gtgcttaacc ttgtcagttc cgcagatact taaactgacg tagtttctaa catagttcaa
    37261 taagttcagt tgttccaggt cagtgggaga agttattaca tagtgcgtgc aatatcacat
    37321 tttctgctat gcaatgaata aaaagttata tcactttttc tcataaaaca ggcagttaac
    37381 ggctctcaat caccccaaag agaagtcaat cc
//
LOCUS       DAZFTN010000042        37125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFTN010000042 DAZFTN010000000
VERSION     DAZFTN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(66..485)
                     /locus_tag="ACHKV2_002248"
                     /pseudo
     CDS             complement(66..485)
                     /locus_tag="ACHKV2_002248"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(479..622)
                     /locus_tag="ACHKV2_002249"
     CDS             complement(479..622)
                     /locus_tag="ACHKV2_002249"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940128.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            complement(683..1024)
                     /locus_tag="ACHKV2_002250"
     CDS             complement(683..1024)
                     /locus_tag="ACHKV2_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940129.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            complement(1109..1306)
                     /locus_tag="ACHKV2_002251"
     CDS             complement(1109..1306)
                     /locus_tag="ACHKV2_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6940130.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            complement(1318..1809)
                     /locus_tag="ACHKV2_002252"
     CDS             complement(1318..1809)
                     /locus_tag="ACHKV2_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6940131.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            complement(1806..2180)
                     /locus_tag="ACHKV2_002253"
     CDS             complement(1806..2180)
                     /locus_tag="ACHKV2_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6940132.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            complement(2270..2422)
                     /locus_tag="ACHKV2_002254"
                     /pseudo
     CDS             complement(2270..2422)
                     /locus_tag="ACHKV2_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            complement(2431..3647)
                     /locus_tag="ACHKV2_002255"
     CDS             complement(join(2431..3339,3342..3647))
                     /locus_tag="ACHKV2_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611429.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFO6940133.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEHNTILKKA
                     NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            complement(3746..4927)
                     /locus_tag="ACHKV2_002256"
     CDS             complement(3746..4927)
                     /locus_tag="ACHKV2_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6940134.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(5123..5217)
                     /locus_tag="ACHKV2_002257"
     tRNA            complement(5123..5217)
                     /locus_tag="ACHKV2_002257"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5181..5183),aa:Sec,seq:tca)
     gene            5510..6892
                     /gene="yicJ"
                     /locus_tag="ACHKV2_002258"
     CDS             5510..6892
                     /gene="yicJ"
                     /locus_tag="ACHKV2_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO6940135.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            6902..9220
                     /gene="yicI"
                     /locus_tag="ACHKV2_002259"
     CDS             6902..9220
                     /gene="yicI"
                     /locus_tag="ACHKV2_002259"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO6940136.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(9273..10982)
                     /gene="yicH"
                     /locus_tag="ACHKV2_002260"
     CDS             complement(9273..10982)
                     /gene="yicH"
                     /locus_tag="ACHKV2_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6940137.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(11103..12494)
                     /gene="xanP"
                     /locus_tag="ACHKV2_002261"
     CDS             complement(11103..12494)
                     /gene="xanP"
                     /locus_tag="ACHKV2_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO6940138.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            12774..13979
                     /gene="gltS"
                     /locus_tag="ACHKV2_002262"
     CDS             12774..13979
                     /gene="gltS"
                     /locus_tag="ACHKV2_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO6940139.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            13982..14848
                     /locus_tag="ACHKV2_002263"
     CDS             13982..14848
                     /locus_tag="ACHKV2_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940140.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(14833..16914)
                     /gene="recG"
                     /locus_tag="ACHKV2_002264"
     CDS             complement(14833..16914)
                     /gene="recG"
                     /locus_tag="ACHKV2_002264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO6940141.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(16920..17609)
                     /gene="trmH"
                     /locus_tag="ACHKV2_002265"
     CDS             complement(16920..17609)
                     /gene="trmH"
                     /locus_tag="ACHKV2_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO6940142.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(17616..19724)
                     /gene="spoT"
                     /locus_tag="ACHKV2_002266"
     CDS             complement(17616..19724)
                     /gene="spoT"
                     /locus_tag="ACHKV2_002266"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO6940143.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(19743..20018)
                     /gene="rpoZ"
                     /locus_tag="ACHKV2_002267"
     CDS             complement(19743..20018)
                     /gene="rpoZ"
                     /locus_tag="ACHKV2_002267"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO6940144.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(20073..20696)
                     /gene="gmk"
                     /locus_tag="ACHKV2_002268"
     CDS             complement(20073..20696)
                     /gene="gmk"
                     /locus_tag="ACHKV2_002268"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO6940145.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            20954..22636
                     /gene="ligB"
                     /locus_tag="ACHKV2_002269"
     CDS             20954..22636
                     /gene="ligB"
                     /locus_tag="ACHKV2_002269"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO6940146.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(22633..23250)
                     /gene="yicG"
                     /locus_tag="ACHKV2_002270"
     CDS             complement(22633..23250)
                     /gene="yicG"
                     /locus_tag="ACHKV2_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO6940147.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(23542..24366)
                     /gene="dinD"
                     /locus_tag="ACHKV2_002271"
     CDS             complement(23542..24366)
                     /gene="dinD"
                     /locus_tag="ACHKV2_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO6940148.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(24587..25450)
                     /gene="yicC"
                     /locus_tag="ACHKV2_002272"
     CDS             complement(24587..25450)
                     /gene="yicC"
                     /locus_tag="ACHKV2_002272"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO6940149.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            25577..26293
                     /gene="rph"
                     /locus_tag="ACHKV2_002273"
     CDS             25577..26293
                     /gene="rph"
                     /locus_tag="ACHKV2_002273"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO6940150.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            26359..27000
                     /gene="pyrE"
                     /locus_tag="ACHKV2_002274"
     CDS             26359..27000
                     /gene="pyrE"
                     /locus_tag="ACHKV2_002274"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO6940151.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(27037..27633)
                     /gene="slmA"
                     /locus_tag="ACHKV2_002275"
     CDS             complement(27037..27633)
                     /gene="slmA"
                     /locus_tag="ACHKV2_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO6940152.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(27740..28198)
                     /gene="dut"
                     /locus_tag="ACHKV2_002276"
     CDS             complement(27740..28198)
                     /gene="dut"
                     /locus_tag="ACHKV2_002276"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO6940153.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(28176..29396)
                     /gene="coaBC"
                     /locus_tag="ACHKV2_002277"
     CDS             complement(28176..29396)
                     /gene="coaBC"
                     /locus_tag="ACHKV2_002277"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO6940154.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            29568..30236
                     /gene="radC"
                     /locus_tag="ACHKV2_002278"
     CDS             29568..30236
                     /gene="radC"
                     /locus_tag="ACHKV2_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6940155.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            30453..30689
                     /gene="rpmB"
                     /locus_tag="ACHKV2_002279"
     CDS             30453..30689
                     /gene="rpmB"
                     /locus_tag="ACHKV2_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO6940156.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            30710..30877
                     /gene="rpmG"
                     /locus_tag="ACHKV2_002280"
     CDS             30710..30877
                     /gene="rpmG"
                     /locus_tag="ACHKV2_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO6940157.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            30975..31784
                     /gene="mutM"
                     /locus_tag="ACHKV2_002281"
     CDS             30975..31784
                     /gene="mutM"
                     /locus_tag="ACHKV2_002281"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO6940158.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(31823..32302)
                     /gene="coaD"
                     /locus_tag="ACHKV2_002282"
     CDS             complement(31823..32302)
                     /gene="coaD"
                     /locus_tag="ACHKV2_002282"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO6940159.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(32310..33587)
                     /gene="waaA"
                     /locus_tag="ACHKV2_002283"
     CDS             complement(32310..33587)
                     /gene="waaA"
                     /locus_tag="ACHKV2_002283"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO6940160.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            34000..35058
                     /gene="rfaQ"
                     /locus_tag="ACHKV2_002284"
     CDS             34000..35058
                     /gene="rfaQ"
                     /locus_tag="ACHKV2_002284"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO6940161.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            35055..36179
                     /gene="waaG"
                     /locus_tag="ACHKV2_002285"
     CDS             35055..36179
                     /gene="waaG"
                     /locus_tag="ACHKV2_002285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6940162.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            36172..36969
                     /gene="rfaP"
                     /locus_tag="ACHKV2_002286"
     CDS             36172..36969
                     /gene="rfaP"
                     /locus_tag="ACHKV2_002286"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO6940163.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         8736 a         9215 c         9849 g         9325 t
ORIGIN      
        1 ttgttgacgt cgggagagtt cagaagagga agtttggcgg agatcacagg agtcgaatat
       61 gattattaat gcattgattt taaatgatta atgcgtgttg gataaatgat tgacccccat
      121 aataaccccc aaatgacttt acatgaggga atgaggctaa aatacacaat tgaatcaatt
      181 cttagagatt tttgtgaagg taacattcct aggtagtgtc tggcaataat atcattaagc
      241 ctgtcaacca gctctggctt tctgaatgtg atgtgtgcgg gcccttctta aagtatttac
      301 ttgtcttcat agcgctcttt accctgaacg gactgtaggt ggtcatccag attaatggtg
      361 atattccctc ggttgttggg gagcggttta ccgctgagca gatgcaacat tctttccagg
      421 tcagctaagc gatctgcctg cattccactg ataagatgaa atctccatct gtcccatctc
      481 accaagttgt tgacgataat tttactgccg aatttacagg gggaattggt tttgtaattc
      541 cgttcggccc tctgaagacg ttgatcaccc cacgctcaaa cacttcatcc ttactctggt
      601 gcagttgttt gaatgcattc atgatattcg cctcgctgat ttccggaata ttatcgtact
      661 ccagtgagcg gtaccatgta tcctagccct gtgcatccat cagactcagc atcccggagc
      721 gttgcatcag gtcacgccag atttcgcagt cgaggttgcg ggtgatggct ttcattgcgg
      781 tttcaggttt ctccatcagc cagcagccgc agtgaaaatc ctgtttcatc gcccggtcca
      841 gggcggtttt gccgccaatg tctgctgctg tcaggactga gatttctgcc agttgccgga
      901 taagcgtttc ggtttgttgc agtgctgcat tccgtaccgt cacaacgtgt cggatactgg
      961 tggagcagat aacgtccgta tgcccggtgt ccacttccgg acgggagatt atgatgcctg
     1021 acattatcgg ttttcctttt ggtttataaa cgaaaacgcc agccctgttc aggctggcgt
     1081 cagtggaatg aagtatggtg agtgagactc accggttctg ttgcagaagc tgcagttggc
     1141 gcagggtacg gacatcttcc ctgacctgtt cgatacaggg caacaatgct gccgaatccg
     1201 ttttatcctc atcgttgtcg aagataattc cggattcgct gtcgatattg tcctgcagcc
     1261 acgcaatcag aatatccagt gctgcttccg gggttaatga tgtcatgttg ttcctagtta
     1321 ccaattaaaa gtgggtaaac ctggcagaca tccggcactg gcatccagat gaatgagact
     1381 gacaccataa cgccggatga gtgtgacgac cagacgacgg aacgtttttg acagacccag
     1441 gcgtttaagc cgcagaaccg ggtacgacca cgcatcggta cgcaacagat agccgttacc
     1501 ggtaaaatga atccattctg attcaccgaa gtcactggtc tggtgtgaca gcgagtacag
     1561 ccaggcgttg tccgtttccg tgatatgtgc ggtactgcag cgtatgccgg taagggcagt
     1621 aaatggtggc gtgcagtctg ctgaggtccg gtcagattca tcaacaacac gaagggagta
     1681 gccgttatcg cagaccatgt taatcagttc agcgagatta acaccatcaa tgtcaacgac
     1741 aattcgcccc atattcagtg cctgtacgtt aacgctgtcg gcttccagtg tcagggccag
     1801 tttcatcatt tcgcctccgg atgcttaccc agggtaatgt tatttaccgt tctgtaattg
     1861 tcgcgggtca tcaggccggt cgccctgcga gcccggagga tatcgatgct gtttattaac
     1921 tgagagcggg tacaggcgct gaatcccggc tggtcggtac gcaccagcac gtatttttcc
     1981 acgagaaagt tcaccgcatc acacagtgaa atacctgcct caatatgctg ctcaatcaca
     2041 cgttcatcag cgaacggtgt gtcattcagc gtgaggccgt agtgctggtc cagcagacgg
     2101 ccaagcagtg tctgccagat ggtgatggga gacgggcagc gcgatgcctc ccgtacgtgt
     2161 gtatcgggta atgttttcat actaaagttt ttcctgatat gcagagatga gaaatgggga
     2221 agtggcgtgg tgaaaacacc aggccggagc agaaggttat tctgatgatt tactttttcg
     2281 tttcgggcgt cggataaaca gccatataaa cgtaacctcg actgccgagg gtatcggctt
     2341 tgcaggtcag cccttttgca tacagcgtga cggtatgctg atggcgggga ttcagctcac
     2401 cgctggtgag catgagttcc agtgtagtgg tcaagtaata ctggccacgg ttttacagta
     2461 aaaatgatat ctgttctctg actcttccgg cgtcagccct ccgttataat ggtgaggcct
     2521 gacgctattg taataattca gaatataact gctgatttgc tgccgggcca cgtctttgcc
     2581 tgtgtagcca tcggttggca cccattctgt tttcagactg cggaagaagc gttccattgg
     2641 actgttatcc cagcagtttc cccgtcggct gacactttgc tttatcctgt aacgccagag
     2701 aagttgttga tatttcagtc ctgtatattg acttccctgg tcgctatgga acatgacgtc
     2761 ccgcggctga ccacgcacct catacgccat ccgcagggca ctgcttatca gggcagtatc
     2821 ggcattcgct gacaggctcc agccgataac cctgcgggca aaaagatcca tgacgaccgc
     2881 cagatagcac cagcgatttc ctgcccagat atacgtaata tctccgcacc ataccctatc
     2941 tggctcgggc acagcgaact ggcgctcaag cagattcggc aggcaggtat gttcctgacg
     3001 agcatttttg tactgatgtt ttccgggctg acaactgctc aggttcagat atttcatcag
     3061 acgcccggca cggtaacggc tcatcgggac gccgttttgg gtcagcattt cagccagcgt
     3121 gcgcgccccc gcagagcccc tactttggtt ccacgcccgg cgtatttcgc tgcacaacct
     3181 gactcgcgcc ggattaaccg tatcgcgtcg ttttcgccag tactggtaac tgctgcggtg
     3241 tatttccaga gcagaacaga ggctgacaac tgagtggctg tcactcagtc tggcaactat
     3301 cgtgaaccgt tcagcgagtc ggacatcaag agcgcggttg tagccttttt taatatcgta
     3361 ttgtgttcct ccaggcggcg aacctgcttt tccagttcgc ggatacgttg ctggtctgga
     3421 gtaataggtg tggcagaggg cgcaatcccc tgacgctctc gcctgagctg gcgcacccaa
     3481 ctctcaagcg tggtagaacc gacattcatc gcttcactgg cttgtcgata tgagtagccc
     3541 ttatcaacaa ttagctgtgc acattccagc ctgaattcag gggtgaaagt acgtttggtt
     3601 ttcttgttca ttaagtcacc tgttttgtgt tgtggtgagg atatcacctt taatcaggtg
     3661 gccaaattta ctgtgccact acaaccatcc gcctgttggg gctggatggt tattcattat
     3721 ttttgtgtca ccgaactttc attcatcagg ccagctcaga aagtgaatca atgtagtcag
     3781 cgtaccattg catcatttca cggcgaccat cgagatactg ggcgtggtta taggtacccc
     3841 tgatggagtt tttatccaca tgagccaact gcatttcaat ccaggcagaa ttaaagcctt
     3901 gttcgtgcag aattgtgctc atggtatgcc tgaaaccgtg ccctgtgagt cttcccttat
     3961 accccagcaa ttcaatgacc tggttaatgc tctccttact aattggcttg cgaggatcgt
     4021 tcctaccgat aaagaccaga ggatgatgtt ttgagattgg ttcaagttgc ttaaatagcg
     4081 caataacctg ctcagagggc ggcacaatat gaggccgttt catcttcatt acttctgccg
     4141 ggatctccca taattttgcc tcaaaatcaa tatcctccca atgcgcgaaa cgcaactctt
     4201 gcgttcgtac accggttaac ataatgatct gagtagctgt cttagtgatg atacttccgg
     4261 tataacccgc cagatcagta aggaagtggg gaagttcatt ggcagtaaga aagggaaaat
     4321 gtactttttt cggtgtagct aaagcactgg caagatcggg agcagggtta tagtcggctc
     4381 gaccagtaac aattgcatag cgaaacacct caccacaacg ttggcggacc ttgcgcattt
     4441 tctccagtgc tccacgtttc tccatcttac gtagtgcttc caatagctcc atcggcttga
     4501 tttcggcaat aggtcgcttg ccgatgtagg ggaagatatc tttctcaaag gtgtcgatta
     4561 tttcatcgcg gtagcgcagt gaccagcgat cggctttcgt ctgatgccac tcgcgagcaa
     4621 cggcctcgaa agtgtttttc aaactcattt gcagcgctat tttttcttct ttttttgcct
     4681 cacccggatc gccaccagcc gcaagtattt tcctggcttc actgcgtttc tcacgtgcgt
     4741 cggcaagcgt tacatcgggg tatacgccaa tagacagcat cttttcttta ccggcgaagc
     4801 ggtacttcat acgccagtat ttggaaccat tagcgttaat cagcagatac atacctccgc
     4861 catcagccag cttatagggc ctttcttttg gcttcgcggt tttcacttta acatcggtta
     4921 gcgccatgac atcctccagc ttttgatgaa tccatcctcc tgggggaatt tccgccagta
     4981 accaacctga tacccccaat gcacccccac aaacaacttg atatgggtta aagtcgtttg
     5041 acctcaggat acaaagaaaa cctgatatat aagggattat aaggggcttt gttgatgtcg
     5101 ggagagttca ggagaggcaa actggcggaa gatcacagga gtcgaacctg cccgggaccg
     5161 ctggcggccc caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg
     5221 cctcgattgc tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc
     5281 ccagatcagt tttttccgcc taatacacgc agcgtattat cacgaaagca ttataattca
     5341 tataaaactc atattttaca atgatatatg agcggaatta acgctaccac gatcacataa
     5401 aacaagaatc ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta
     5461 caaaatgcgt tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaagagtga
     5521 agtgttgtcc gttaaagaga aaattggtta tggcatggga gacgccgcca gccacattat
     5581 tttcgataac gtaatgttat atatgatgtt cttttatacc gataattttg gcatacctgc
     5641 cggatttgtc ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg
     5701 catggggttg ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact
     5761 gtgtggcgca ctgccatttg ggatcgtctg tgtactggcc tatagcacgc cagatctcag
     5821 tatgaacggc aaaatgatct atgcagcaat tacttacacc ctacttacct tactttatac
     5881 cgtcgtcaat atcccttact gcgcattggg tggtgtaatc accaatgacc cgactcagcg
     5941 tatctcgctg caatcctggc gttttgtgct ggcgaccgcg ggaggcatgc tctctactgt
     6001 tctgatgatg ccactggtta atttaattgg cggtgataat aaaccactcg gtttccaggg
     6061 cggtatcgcg gtcctttccg tggtggcatt catgatgctg gcattctgtt tcttcaccac
     6121 taaagaacgc gttgaagcac cacctacaac gacgtctatg cgggaagatt tacgtgatat
     6181 ctggcaaaac gaccagtggc ggattgtcgg tttactaacc attttcaata tcctggcggt
     6241 gtgcgtccgc ggtggagcga tgatgtatta cgtcacatgg attttgggta cgccggaagt
     6301 gtttgtcgct tttctcacca cttattgcgt gggtaacctg attggttccg cactggcaaa
     6361 accgctgacc gactggaaat gtaaagtcac tatcttctgg tggacgaacg ccctgctggc
     6421 agtgattagc ctcgcgatgt tctttgttcc tatgcaggcc agcatcacta tgtttgtctt
     6481 catcttcgtg attggtgtat tgcatcaact ggtgacacct atccagtggg taatgatgtc
     6541 cgataccgtc gactacggcg agtggtgcaa tggtaaacgc ctgaccggga tcagttttgc
     6601 tggcacgctg tttgtgctca aactggggtt ggccttcggc ggcgctctta tcggctggat
     6661 gctggcttat ggcggatatg atgcggcaga aaaagcgcag aacagcgcca cgattagcat
     6721 cattattgcg ctattcacga ttgttccggc gatctgttat ttgctgagcg cgattatcgc
     6781 taaacgctac tactcactca cgacgcacaa tctgaaaacc gttatggaac agctggccca
     6841 gggcaaacgc cgttgccagc aacaattcac ctctcaagaa gtgcagaact aaggaacggc
     6901 aatgaaaatt agcgacggaa actggttgat tcaacctggc ctcaatttga ttcacccgct
     6961 tcaggtgttc gaggttgaac agcagggtaa tgaaatggtg gtctatgctg ccccccgtga
     7021 tgtgcgtgaa cgaacctggc agcttgatac gcctttattt acgctgcgct ttttctcccc
     7081 acaggaaggt attgtcggtg tacggattga gcattttcag ggggcgctga ataacggtcc
     7141 tcattatccg ctcaatattt tgcaggacgt gaaggtcaca atcgaaaaca cagaacgtta
     7201 cgctgagttt aaaagtggca acttaagcgc gcgtgtcagc aaaggtgagt tctggtcact
     7261 ggattttctg cgcaacggcg aacgtattac cggtagtcag gtgaaaaata atggctacgt
     7321 gcaggacacg aataatcaac gaaattatat gtttgagcgg ctggatcttg gcgttggcga
     7381 aacagtttac ggtctgggag agcgctttac tgccctggtg cgcaatggcc agacggtaga
     7441 gacctggaac cgggacggcg gcacaagtac tgaacaggcg tataaaaata tcccgttcta
     7501 tatgactaac cgtggctatg gggtactggt caatcatcct caatgcgtct cttttgaagt
     7561 gggatcggag aaagtctcca aagtgcagtt cagcgttgag agtgaatatc tcgaatactt
     7621 tgttatcgac ggcccgacgc cgaaagcggt acttgatcgt tatacccgtt ttactggtcg
     7681 tccggcgctg ccgcccgcgt ggtccttcgg cctgtggcta accacttcat ttaccaccaa
     7741 ctacgacgaa gcgacggtaa acagctttat cgatggtatg gcgggacgca atctgccact
     7801 gcatgttttt cactttgact gtttctggat gaaagccttc cagtggtgcg attttgagtg
     7861 ggacccgctg actttcccgg acccggaagg gatgatccgc cgcctgaaag cgaaagggct
     7921 aaaaatctgc gtctggatta acccctatat cgggcaaaaa tcccctgtct ttaaagagtt
     7981 acaagagaaa ggctatttac tcaaacgccc ggacggttcg ttatggcagt gggataaatg
     8041 gcagccaggt ctggcgattt atgactttac caatccggat gcctgcaaat ggtacgccga
     8101 caaactgaaa ggtctggtcg cgatgggcgt tgattgcttt aagaccgact ttggcgaacg
     8161 tattccaatc gatgttcagt ggtttgacgg ttccgatccg cagaaaatgc ataaccatta
     8221 tgcgtacatc tacaacgaac tggtgtggaa cgtgctcaag gacaccgttg gcgaggaaga
     8281 agccgtcttg tttgctcgct cggcctccgt tggtgcgcag aaattcccgg tacactgggg
     8341 tggtgactgt tacgctaact acgaatcaat ggcggaaagc ctgcgcggtg gtttgtctat
     8401 tggcctttca ggttttggtt tctggagcca cgatatcggc ggctttgaga ataccgctct
     8461 ggcgcacgtt tacaaacgct ggtgcgcgtt tggtttgctc tccagccata gccgtttaca
     8521 tggcagcaaa tcttatcgtg tgccgtgggc ctacgatgat gagtcctgtg atgtggtgcg
     8581 cttcttcacg caactgaaat gccgcatgat gccgtatctg tatcgtgaag ctgctcgtgc
     8641 gaacgcgcgg ggtacgccga tgatgcgggc catgatgatg gagttcccgg acgatccggc
     8701 ttgtgattac cttgaccgtc aatacatgtt aggcgacaac gtgatggttg ctccggtgtt
     8761 cagtgaagcg ggcgatgtgc agttctactt gccggaaggt cgctggacac atctgtggca
     8821 caacgatgaa ctcgatggta gtcgctggca taaacagcag cacagcttcc tgagtctgcc
     8881 cgtttatgtg cgtgataaca ccctactggc gctgggcaac aacgagcaac gtcccgatta
     8941 cgcgtggcac gaaggcacgg cattccagct ctttaatcta caagacgggc atgaagccat
     9001 ctgtgaagtg cccgctgctg acggttccgt tcttttcacc ctgaaagcgg cgcgtactgg
     9061 caacacgatt actgtgactg gtgcgggcga ggcgaagaac tggacactgt gcttgcgcaa
     9121 tattgtgaaa gtaaatggtc tgcaaggtgg ttcgcaggct gaaagtgagc aggggctggt
     9181 ggtgacgcct caagggaatg cgctgacaat tacgttgtaa tctattgatg gaatgccggg
     9241 tacgtctgag tcgtacccgg cgagattgat ttttactgca ccggctctgt cgatgtaact
     9301 tcgctactgg aaacgacgcc cgcattcatg gtttgagtca cttgctgctt tgcggcgttc
     9361 acggcatgga gttgcccgct aaccgtaggt ttcagcggga cattggcctg aatatcgcca
     9421 ctggcggtaa gctgaatatt gccgtcgcca gtcaacggta attcaggcca tccccattgt
     9481 tgcaaaatat tcaccggcac accgcgtcca ttcaggctga tatgggtctg acgttgtggc
     9541 gtttgtgaaa cactggcagt ggcttccaga atgccttttt cagtaaatgc actcagttcg
     9601 ctgatattca ccgtgctgct gttggcggtc agcgccagcg acgggcgacg aacatcaaca
     9661 cgattgaatg tcgcggcggc ggcattcaga ttcgccgagc cactccagac gccccattta
     9721 tgatcggtaa ccagcgtcag gttggcaccg taaccatcga gcgtggtgag ctgccacggg
     9781 aagtcagggt cgatatcaat gatcagattg cggctggcgc taaatctctt cagttgcagg
     9841 ctgtttaacc aaccgggtgt cgtttccatc cacaactgtt gccagttttt cggcaaggta
     9901 tattccagcc cggcaattgc cgcatcatca aggatcaacg ttttcccgtc acgcagccag
     9961 ttccctgacg ttctgaccat acccccttcc cagcggctgg tgaactggcg cagcgctacg
    10021 ccctgcgggg aaaattccgc gtttataatc gggtcaaata aatgcagcga accataaatg
    10081 aactcgctag cgttcatcga cagtttgcct tcttgtgtct gccagtcatc tttactgaag
    10141 gtcatgttgc gcaagctgag atcgaggtcg gtcaccgccc agtccggacc ttgcaaacga
    10201 gcatcgatca cttccaggcg accaatctgc aacgacggga cagagcgtaa tggcgcaaag
    10261 aagtcggtta gcgatttttc gctttgtaga cggatatccg ccatgcgcag attttccact
    10321 tgccagctgc cgtcggcgtt acgctgcgcg tttccggtta atgtcccgcg ggcgatgtcg
    10381 gcacccaggt tagtcagcgt aacgcgatcg ttatcaatac tgccttcaat cagtacattg
    10441 gtggcaggaa catcattgag cgaaagcgat ccggcactaa actgaatctg cgccttcgta
    10501 cccagcactt taccggcttc tggtgaccac ggaaccacgc cgccatttac ccgctgcgcg
    10561 ctcagtttcc attcgctatt cgggctatta aacgccatat cacgcagttg cagacgatcg
    10621 gctttgaacg gtagcggcgc ggtctggtcg gtgagattca gcgtcccgtt ttccagcagg
    10681 atggtatcga catggcgtgg ttcggtcagt tgccgactgc ttagcgcaat gtcgacactt
    10741 tttgccacca gggtcgcggg ctgaccatca cgaccaaacg tgacgttctc cagcacgata
    10801 tgagatggcg cggaaaaacg gtgatccatc gccccgaagg ccagatgata gtcgctattc
    10861 tcggaaaccc atgcgctgat atgttctgct ccccagcggg tttgcagaag aaaatagagg
    10921 ccagcgatca ccactaacag agcgatgaga atgtagagaa gcagcttccc aataaatttc
    10981 atggtcttcc atcccgtgaa atgcacataa gggagttatg cacgatttac gcgcaatcct
    11041 caaggcggga atggtgaaag aggtgagact gcggcggtaa ttatcttacc gccgccagtg
    11101 aattactgtt tttctggtgg gaaaatcaga ttcagcacga tggcagtaat accgcccgcg
    11161 gcgatcccgg aggagagcag gtttttcagc cattcagggg caaactgcaa aatcagcggc
    11221 tgctgagaca cgcccagacc aaccgccagc gacagcgcga taatcagaat cgcccgacgg
    11281 ttcagcggct cacgagaaac gatacgcaca ccggaggcgg cgatggtgcc aaacattaca
    11341 agcgttgcgc cgcccagaac cggttctgga atgtgttgta caaaaccgct cactgccggg
    11401 aacagaccca gcacgatcaa catcagcgcg acgacaaaac cgacatagcg gctggcaaca
    11461 ccagtcaact ggatcactcc gttgttttgc ccgaagcagg agttcgggaa ggtgttaaac
    11521 accgccgaaa caaacgagtt caggccgttt gccagcacgc cgcctttcag gcgtttcatg
    11581 tacagcggac cggacactgg ctgttcggaa acgtcagagg tcgccgtgat atcgccaatg
    11641 gtttccagcg aagtgatcat aaagaccagc atcagcggca gcagcagact ccattcaatg
    11701 ccaagaccgt aatagagcgg cgttggcacc ataatcagtt cttgcgtcat cggttcgttg
    11761 ctttctggca acatgcccat aaaccacgcc agcgcatatc cggccgccat cgcaatcacc
    11821 agtgaggcca cgcgtaagta agggttacgt tgacggttaa gcaggataat taaggctaag
    11881 accacgcctg ccagcagcag atttttcggt gcgccgaagg tgttatcgct catggctgcg
    11941 taaccgccgc caatggacgt taacccaacc tgaattagcg acaggccgat aatcatcacc
    12001 acaacgccag aaaccagcgg cgtaataatg cggcgcgcca gatgcagaac gcgggagatc
    12061 accatctcgg tgcaacttgc cagcatcaac gtgccgaaca aagccgccat catggtagga
    12121 acatcagcac caccggtttt cagcgcggta ccgcccataa tcagcggggc aacaaagttg
    12181 aagctggtgc cctgaataga caacagcccg gagccaaccg gaccccaggc cttaatttga
    12241 ataatcgatg ccacaccgga ggcaaacagc gacatactaa taatgtgttg cgtgtcttgt
    12301 gccggtaaac ccagcgcctg gcagattaat agcgctggcg tgatcaccgc aacgaacatc
    12361 gccagcagat gctgacaggc ggcaaacagg gtttgaggaa gcggcggacg atcttcaaga
    12421 cggtaaatca gttcgctgtt ttgagtctgc gcaaccggtt gcgcattttc tgactcgagg
    12481 gtggaaacag acatcgaaaa caatcccgtg gtagcaaagc gggcatttta gctgaccgaa
    12541 agagaaaagc aaacgtttgc cacatcctca tctaaatatt taaatgagat aaatgcactt
    12601 ttttatataa cttttggtat ttttctgctt aaaatccatg ccatttgttc attaacagct
    12661 gagaatttga cccaggaaag cataactcga tacccgtcat agttcatgaa gttgccactg
    12721 cgcgatgaag tatgacgagt atgaaagagt gatgcggata caaaggagta actatgtttc
    12781 atctcgatac tttagcaacg cttgttgccg caacgctgac gttgttgctc gggcgtaagt
    12841 tggtccattc cgtctccttt ttgaagaaat acaccatacc ggaacctgtt gcgggtggtt
    12901 tgttggtggc gctggcgcta ctagtactga aaaaaagcat gggctgggaa gtcaactttg
    12961 atatgtccct gcgcgatccg ttaatgctgg ctttcttcgc caccattggc ctgaacgcca
    13021 acattgccag tttgcgtgcc ggtgggcgtg tggttggcat cttcttgatt gtggttgttg
    13081 gtctgttggt gatgcaaaat gccattggca ttggtatggc tagcctgtta gggcttgatc
    13141 cgctgatggg gctgttggcc ggttctatta cgctttccgg cggtcacggt acgggcgctg
    13201 cgtggagtaa attgttcatt gaacgttatg gcttcaccaa tgcgacggaa gtggcgatgg
    13261 cctgtgcaac gttcggtctg gtgctgggcg gcttgattgg cggtccggtg gcgcgctatc
    13321 tggtgaaaca ctccaccacg ccgaacggta ttccggatga ccaggaagtc ccgacggcgt
    13381 ttgaaaagcc ggatgtgggc cgcatgatca cctcgctggt gctgattgaa actatcgcgc
    13441 tgattgctat ctgcctgacg gtggggaaaa ttgttgcgca acttttggct ggcactgctt
    13501 ttgaactgcc gaccttcgtc tgtgtactgt ttgttggcgt gattctgagc aacggtctgt
    13561 caatgatggg cttttaccgc gtctttgagc gtgcggtatc cgtgctgggt aacgtaagcc
    13621 tgtcgttgtt cctggcgatg gcgctgatgg ggctgaaact gtgggagctg gcttcgctgg
    13681 cgctgccgat gctggcgatt ctggtggtac agaccatctt catggcgttg tatgccatct
    13741 tcgttacctg gcgcatgatg ggcaaaaact acgatgcggc agtgctggct gcgggtcact
    13801 gtggttttgg cctcggtgca acgccaacgg caatcgccaa catgcaggcg atcactgaac
    13861 gctttggccc gtcgcacatg gcgtttttgg tggtgccgat ggtcggtgcg ttctttatcg
    13921 atatcgtcaa tgcgctggtg attaagctgt atttaatgtt gccgattttt gccggttaaa
    13981 aatgaaaaag cgtcggaagg aaccggaaac gctacgtgaa cactgtcgcc atatttttgg
    14041 tgatgagccg ccagtgctct gtgtatggga aacggaattt gattacgcag atgccgaact
    14101 caaggcgctg gcggctaaag agtggcagca gatcagcgaa tgggatttga gtgcttacta
    14161 tgtactcaat ctcgtttata acgaaccgat gcaaatagag ttgtttcgct atctgtttcc
    14221 gctctgcctg gcgcaatggc atgaaaccgt tttagctggc ggttatggtg atcacttcga
    14281 agaatcctta atgaaggcat tgtgtcggcc ctatctctgg caggagatga tgaatgcttc
    14341 ccaacgccag caggtacggc aatttttact ggacaccgcg ttgcagcgta tggataacga
    14401 gcgtggtttc aataacgttc tttgctggct ggcggtgttc aatactctgg gtggggccgc
    14461 gccgttaatt cgttctctct ggtcgcgctg gtgggcgctg gatacgccag gaaaagcggt
    14521 gtgtgctatt cagtatgccg cgcatctaat ttatcccata gaagccaatc cgttatggtc
    14581 gcaagaatgg ataggctggg ggcatccatt aggccataaa gatggctggt cctctgataa
    14641 tcgcgctttt cttcgccaaa tgttaacacc tgaaatgatt gtggctggcg tacaggcagc
    14701 ggctgaaatt ctgcgtggtg aaccggaagg cgcaatggcg gcgcgaattg cacaggatgc
    14761 gtatgaggca atggatatcc tcactatcca gattgaagat ttactgcgtg acttgtcttg
    14821 tgatgagtct ggtcatgcgt tggagtaacg ttccgtctcc ggcatccagc gttctatcag
    14881 ggcttttgct tgttgtgggt aacgttcgtg aatatgtcgc gccaggcgct gaacttccgg
    14941 gatcatcgcc tgatcgcgca gtaaatccgc cactttaaac tcagcattac ccgtctgacg
    15001 cgtacctaac agttcacccg gtccgcgaat ctctaagtct ttctgtgcaa tcacaaaacc
    15061 gtcgttgctg tcgcgcagca cttgcagccg aatttgcgcg gttttggaga gcggcgtttt
    15121 gtagagcagc acgcagtgag aagccaccgc accacgacct acacgcccgc gaagctggtg
    15181 taactgcgcc agacccagac gctcagggtt ttcgataatc atcaggctgg cgttaggcac
    15241 atcaacaccg acttcaataa cggtagtggc aactagcaga tgtagctcac cttgtttaaa
    15301 cgacgccatc accgcctgtt tctcggctgg tttcatccgc ccgtgtacca ggccaacatt
    15361 caattctggt agcgccagtt tcaactcttc ccaggtagct tccgccgcct gcgcttccag
    15421 taactctgac tcttcaatca acgtacaaac ccagtacgcc tgacgacctt cagttatgca
    15481 ggcgtggcgc acgcggtcaa tgatgtcggt acggcgggta tcaggaatag cgaccgtagt
    15541 cactggcgtg cggcctggcg gcagctcatc tatcaccgag gtatcgagat cggcatacgc
    15601 cgtcattgcc agcgtgcggg ggatcggcgt ggcggtcatg atcaactgat gcggatggaa
    15661 gccttgctgc tggcctttct cccataacgc cagacgctga tgcacgccaa agcgatgttg
    15721 ttcgtcgata atcaccagcg ccagaccgtt aaactgcacc tgttcctgga agatggcgtg
    15781 cgtaccaaca atcatctgca cctgaccgct ggcgatggct tcctgctgag acagtcgcgc
    15841 tttgcctttc tgcttaccgg cgagccagcc cacttcgata ccgagcggtt caaaccagtt
    15901 gcggaagtta ttggcgtgct gttcggcgag taattcggtt ggtgccatca atgccacctg
    15961 tttgccgtgg gcaatcgcac gcagcgcggc gagggcggcg accagcgttt taccggaacc
    16021 tacatcgccc tgtaccaggc gcatcatcgg cacatccagc gccatatcgc gctcgatctc
    16081 cgccactacg cgtgcctgtg cgcccgttgg cttgaacggt aaggctgcga ggagtttatt
    16141 tttcagcgca tcattggcgc tcagcggctg ggcatgaaaa cgctgtgctc cggcacgtaa
    16201 ggctaacatg ctgaggttgt gcgccagcag ttcttccaga atcagacgac gttgcgctgg
    16261 atgctgcccg gtttccagat cgctaagctg tagcgtcggt ggcgggcggt gcaaagtgcg
    16321 caacgcttcc ggtagcgtca tcattccttg tgacagttcc ggcggcagga gttcttcaat
    16381 ggcgcaggtg tcgagcagat ccagcgcctg gtcggttaat ttacgcagcg tggcctgctt
    16441 tacgccttcc gttgttggat aaaccggcgt gagcgtttcc tgtaattctg gcgtgctgag
    16501 atcgccctgc acgcggtatt ccgggtggat catctccgca ccatatttac cgcgctttgc
    16561 ttcgccataa gccagtacac ggcggcccgt cgccaggcta tttttcattg ccgcgctgaa
    16621 attgaaaaag cgcatggtga ggatgccgga accgtcgctg atctggcagg tcatcatccg
    16681 ccgaccgccg aaggagatat tgcagttcag cacttcgcct tccaccgtgg cataaacgcc
    16741 cggcagtagt tctccgatgg ggtagagatg ggtgcgatct tcgtagcgca ggggaaggtg
    16801 taagagtaga tcctgcacgg tatgcaggtt gattttcgcc agtttgttac taagtgctgc
    16861 gccaacgccc gttagggaac tgagtgggac agcatctaac aggcgacctg tcatggcact
    16921 taccctgcag cctgcatagt agcccaccag tcggcatcag cttcgatctc gccttgctga
    16981 ttgacgtggg gataaggcag gcctttgcgt tttgcgactt tcgccagcac cggatagccg
    17041 ccttcaaaca acaggcgttg ttgctctgct tccggcaaca tgctgttttc acgcaggtac
    17101 atgcctgcat tttgccgctg acgctgggct tcgtaaagaa tgagggctga ggcaacggaa
    17161 acattcagcg actgcaccat gccgatcatc ggaatgatga tgtcctgatc cgccagggcc
    17221 aatgcttcct gcgtgatgcc cgttttctcc tgtcccatca aaatgcaggt cgggcgagtg
    17281 taatcaattt cgcggaaatc gacagcgtta tcagaaagat gggttgccag aatctgcatg
    17341 ccctggcctt tgagatgagc gacggcatcg ccaatggtgc ggtgtgtttt cacctgtacc
    17401 cagctgttac tacccgccgc tgccgaagcc atggtgcgca tgcggctacc aggccagacg
    17461 gcgtgaactt catgtacgcc aacggcatct gcggtacgaa taatcgcaga aacgttatga
    17521 ggtttgtgga cctgctccat gcagacggtc agatcaggct gccgcctggc gagcatttcg
    17581 cagatgcgtg cataacgtgt tgggttcata aaacattaat ttcggtttcg ggtgacttta
    17641 atcacgtctg gcatcacgcg gattttgcgc atgatattcg ccagatgcac acggtcacga
    17701 gcggtcagac gaataaaggc gctgtagacg cgaccatctt tctcttccgt attcaaactt
    17761 tgaatattcg aggtcgtggt gttaattgcc gccgtcaggt ttgccagcgc accctgatga
    17821 ttgaacatct ccaccttgat ttcggtgatg aactcctgcg ccgtctcttt atcccattcc
    17881 acagccataa acttctctgg ctctttctgg tagccacgga tgttacggca ggattcatgg
    17941 tggatcacca gacctttacc ggggctgacg tgggcgataa tcgggtcgcc aggaatgggg
    18001 cggcagcatt tcgcaaaggt gatcagcacg ccatcagcgc ctttgatcgg cagatgcccg
    18061 tggctttgag tcgccggtgg aatggaggcg tccccatgtt gtagattttt cgcgaccacc
    18121 acgctcattg cgttaccaag gccgatttct gccagcagat cgtcaagcgt tgccagcttc
    18181 atgcgatcca gctcgcgctg aatattttcc tgcgggattt cattgagctt acggctacca
    18241 cccaaagcat ggttgagcag acgacggccc aggcttacag aatcatcacg cttgaggttt
    18301 ttcagcaact gacgaatttt ggcgcgcgct ttcgagctaa cgacaaagtt cagccaagcg
    18361 gcattcgggc gagcgcccgg agcggtaatg atttcaacgg tctgaccgct agtaagcggc
    18421 tgcgacagcg ggtaaggctg gcggtcgacg cgtgcgccca cgcaggcatg gccgatatcg
    18481 gtatgcactg cataagcgaa gtcgacgggc gttgcaccgg caggcagctc gacaatgcgc
    18541 ccttccggtg tgaaaacgta aatctcatcc gggaagagat cggatttaac gctctcgata
    18601 aattcaaacg aactaccggc gctctgttgc agctccagca ggctttgcat ccagcgctgg
    18661 gcgcggattt gtgcggtagt actggtttcg ccgtgctctt tataagccca gtgcgcggca
    18721 acacccatct ccgccatctg atccatatct tcggtacgga tctggacctc aaccggcacg
    18781 ccgtgcgggc caatcatcga ggtgtgcaac gactgatagc cgttcgcttt tggaatggcg
    18841 atatagtctt tcacgcggcc cggacgcggc ttgtacaggc tgtgcatctg gcccagcacg
    18901 cgataacagg tgtcagaatc attgacgatc acgcggaaag cgtagatgtc catgattgag
    18961 tgaaaacgct gctctttgag caccatttta cagtaaatcg aataaagatg cttttcgcga
    19021 ccactgacgc ggcacggtat tcccgcttcc tgcaaacgcc cttcgatttc agaaagaatc
    19081 ttctggatca tctctttacg gttgccgcgc gcggctttca ccacttcttt gattacgcga
    19141 taacggttgg gatacagcgc ctcaaaaccc agctcttcga gttcggtttt aatgtggtgg
    19201 atacctaaac ggtgcgccag cgggctgtaa atttcgagag tttcacgggc gatgcggcga
    19261 cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt gggtacggtc ggcaagtttg
    19321 atgaggatga cgcggatatc ctgcaccatc gccataatca tcttgcgaaa gttttcggcc
    19381 tgcgcctctt tcttatcgcg gaacttgagt ttatcaagtt tcgacacccc ctctaccagc
    19441 tcggcgacgc ttttaccaaa aagctgttcc atatcctggt aggtggcggg agtatcttca
    19501 atcacgtcat gcagcagcgc cgccatcagc gtttcatagt cgagtttcat ctcggccaga
    19561 atgcaggcaa ccgctaccgg gtgcgtgata tagggttcac cgcttgaacg tgtttgcccc
    19621 tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac gcttgatttg gtcttccggc
    19681 aggtaggttt gaatcagttg attcaggctt tcaaacagat acaagggcga cccgctttgt
    19741 gattaacgac gaccttcagc aatagcggta acggcttgta attcagcggc ttcctgctct
    19801 tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt tgatcagacc ttcttcgatt
    19861 tcgcgcagcg cgattacagt ggttttatcg ttttcttccg gtaccagcgg atcctttccg
    19921 cctacctgca tctgacgagc gcgacgcgcg gcgaccagta ccaggtcaaa acggttacca
    19981 attttctcta cagcgtcctg aacagttacg cgtgccatac ttaaaaagct ccacaggtga
    20041 agaaatgact gggcatgata ctgaaatcag gttcagtctg ccaacaattt gctgattaaa
    20101 gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt cggcgcgaat aatggtcttc
    20161 aaatcggtca acgcggtatc gaagtcatca ttcacaatca ggtaatcata ttcggcgtaa
    20221 tggctcattt ctgcaacagc ttgcgccata cgctttgcaa tgacctcttc gctgtcctga
    20281 tcgcgaccgc gtagacggcg gtccagttca attttagacg gcggtaaaat aaagatactc
    20341 cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcgccct gccagtcgat atcgagaaaa
    20401 acatcgacac cggtcgccag tacttgctca atggcctcac gcgaagtgcc atagtaatta
    20461 ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa tcatttcttt aaattcatca
    20521 tgattaacaa agaaataatg ttcaccgtgg acttcaccag gacgcggttg gcgtgtggtg
    20581 tgtgaaacag aaacctgggt gtcatacaac ggttgggttt ttaataaagc ctgaatcagg
    20641 ctggatttac ccgcgccact gggggcagaa acaatataaa gcgtgccttg agccatgagt
    20701 atctttcgta tgtgattagc gaaataaagc ctacatacga gcttattata cacggcgcgg
    20761 ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt tgttgcgttt tgcgtagctg
    20821 tttccgcatt tgcctgtgat ttactgcaat aaagacgatt ctcaggaagc gtcctcgcag
    20881 actccgtttt tgccactcgg ctttgccttc accacgttgt atatctgcca gcctgactac
    20941 agcggaggtg aagatgaaag tatggatggc gatattaata agtatcttgt gctggcaatc
    21001 atctgtgtgg gcggtctgtc cggcctggtc gccagccaga gcacaggaag aaattttccg
    21061 cctgcaacag caaataaaac agtgggacga tgactactgg aaggaaggaa aaagtgaggt
    21121 ggaagacggt gtttacgatc agttaagcgc ccgtcttacg cagtggcaac gctgctttgt
    21181 gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt gcggttatgc atccggttgc
    21241 ccatacgggt gtacgcaaaa tggcggataa aaacgcatta agtctgtgga tgcgagaacg
    21301 tagcgatctt tgggtgcagc caaaagttga tggcgttgcg gtaaccctgg tttatcggga
    21361 cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg aaaggcgagg actggacgca
    21421 gaaagttagc ttaatttccg ctgtgccgca aaccgttagc gggcctttag ccaacagtac
    21481 gcttcagggg gaaatatttc tccagcgcga ggggcatatc caacaacaaa tggggggaat
    21541 aaatgcccgc gcaaaagttg ctggcctgat gatgcgccag gacgatagcg acacgctgaa
    21601 ttctctgggc gtttttgtct gggcatggcc ggatggaccg cagttaatga ccgatcgttt
    21661 aaaggagctg gctaccgcag gttttactct aacgcagagg tatacccgtg cggttaaaaa
    21721 tgctgatgag gttgcgcgcg tacgcaatga gtggtggaaa gcgaaattac ccttcgtcac
    21781 cgatggcgtg gttgtgcgag gggcgaaaga acccgaatcc cgccattggc taccaggcca
    21841 ggcagaatgg ctggtggcct ggaaatatca acctgtagct caggttgcca aagtgaaggc
    21901 gattcagttt gcggtgggta agagcggtaa aatatcggtg gttgcgtcac tcgcacctgt
    21961 catgctggat gataaaaaag tccagcgggt gaatattggt tccgtcaggc gctggcagga
    22021 gtgggatatt gcgcctggtg atcagatcct cgtcagcctt gccggtcagg gtattcctcg
    22081 cattgatgat gtggtctggc gcggtgcaga acgtacaaaa ccgacaccac cagaaaaccg
    22141 ctttaactcg ttgacctgct actttgcttc tgatgtttgt caggaacagt tcatttcacg
    22201 cttagtctgg ctggggtcaa aacaggttct ggggctggat ggcattggtg aggccggctg
    22261 gcgcgcgctg catcagactc atcgctttga gcatatcttt tcctggcttt tattaacgcc
    22321 agagcaatta cagaacacgc cggggatcgc gaaaagtaaa agtgcgcagc tatggcatcg
    22381 gtttaatctg gctcgtaagc agccttttac tcgctgggtg atggcaatgg gaataccgct
    22441 aacccgggcg gcgcttaatg ccagtgatga acggtcctgg tcgcaacttt tatttagcac
    22501 ggagcagttc tggcagcagc tgccggggac tggatcgggg cgcgccagac aggttattga
    22561 atggaaggaa aatgcgcaaa tcaagaagct gggcagttgg ctggctgccc agcagatcac
    22621 aggttttgaa ccttagtgtc cttcgtggct gtagtaaaag accggtaatc ccagcttaag
    22681 gcgtagcgcc agtaagcggg caaaaaagcc gaataccagg gtggagataa ttaccacatc
    22741 atggttagaa acatagtgtt gcagcgcgat gtacagcacg gcggaggcaa atgagacacc
    22801 ggcatacaac tctttctgga ataccagtgg gatacgttta cagaacatat cgcgcaaaac
    22861 gccgccaaac acgccggtgg ttaccgccgc gacaacggca ataattgggc cgtgccccat
    22921 atccagtgca acctgtgcgc cgatgataga aaagaccacc agtccaagcg catcgagcac
    22981 cagaaacact ttgcgcaggt aaggcattac aggagcaacg atagtggtga gtactgcggc
    23041 ggtagcgacg ataatcacat attccgggtg tttgacccag ccgagcggat agtggcccag
    23101 cagaatatcg cgcactgacc ctccgccaat tgcggtggcg gtcgcaataa taattacgcc
    23161 aaatgtatcc atgcggcgtc gcccggccgc cagagccccc gtcatggctt cggcagtaat
    23221 gccaaccaaa tacaaaatgt gtaacagcat ggttctctcc ctaaaaatct gtctggcagg
    23281 gtagcgattt gtgcgtattg tcacgattga gattttttaa ggtgagagga aattgataac
    23341 ttgtagttat gtgaaatcgc tattttctgt agcgtagatg cattcttctg acttcttgtc
    23401 tgagaaaatg cataagaaaa aataggttaa aattgagggg tggaaaagaa aggtgcaatg
    23461 tttagccatt gtgttcaccg cacctggact ttggcttacg catcgggtat ctcaatcaac
    23521 aaaaagagtt tttagtttct gctatttctt ctctgtaatt tttacactgt tttccaactg
    23581 cttgatgctg acctgcgggg tcggcaactc ctcaggcatg gttccgccaa gttcctgaat
    23641 ggtttgcctc accttgctac ccacatcaaa gtgtgtggta ttagcttgct gttttgaatt
    23701 cacgccatcc cgcttgagtt tttcttctgt ttgggtagct cgaaagagat tagccgccag
    23761 ttctgttgaa cccatatgat ccaggatctt ctgactcttt ttcagcccct tcagttggtg
    23821 gatggctttc tgatctaatc caccatacag cccctggtaa ccatgattct gaaatatggc
    23881 gaaatcagta gctgtcgcta ccgcagcttg ctgtgctgcc tcaaccaatt gtttgttatg
    23941 ttctttcaac tcatttctca gaaacagacg cttttcgtct tcacgaagtt gcttaaatgc
    24001 ttcatcgtca gcaagctcct gccgtcgggt ctggatagca aaataagtct gccctgccgc
    24061 aatgaccggt ttcgcagggt cgccgttttg tactaccagg taacaggcat agcgggagag
    24121 atgaacatct tcaagttctc gttgagcccc tgaacctaat acaaccattt tggtggtttc
    24181 aacgaaatgg tcagatgcag cttgattgct ggcttcgcac gcttgagtcg cgcgggccag
    24241 taccttttga aaattgcgcc agtcccgata atccagtaac ggtgccaatt ctctagctga
    24301 ccaaaattct tgcccttcag tgccataatg ccgtatttct tcaaaaggtt gatgatgttc
    24361 gttcattgcc cactccatgt gatatcgatt gcaaagaaag taaatctgta actgtattta
    24421 tatacagttg tgagtatctc acgttctcag tgtgctgggc aaaaaaacat cacaaaatct
    24481 ggagcaggca cgcaaaacag aattgaagtt gcggggaaga aaaatgccgc tctgtttgag
    24541 tgggcggcat ttgtgaaggc tgaaaagtaa gaattggcgt tacgagttat tcaatgttct
    24601 gaatctgctc gcgcatctgc tcaatcaaca ctttcagctc aatcgcggag tttgtcactt
    24661 cggcattgat agacttcgac gcaagagtgt tcgactcgcg gttgaattcc tgcatcataa
    24721 agtccagacg acgaccaacg gcttcttttt tcttcagaat gttgtaggtc tctttgacat
    24781 gcgcttccag gcggtccagt tcttcggcaa cgtcaattcg ttgtgccagc agaaccagct
    24841 cctgctccag acggttgttt tccaactgaa cctgagcatc ttccagcttc gcgaccagac
    24901 gctcacgctg ccattgcagg atttccggca tatgggcgcg gactttgacc acttcggcgg
    24961 tgacgccttc cagacgctgc tcgatcaatg ctttcagtgc ctgaccttcg gtttcgcgcg
    25021 cgacaataaa gtcgtccagc gtaccatcca gcgccgcgag aatttcagcg gcaatggcgt
    25081 caagatcctg ctcctgggct gccatcacgc ccggccagcg tagaatatca accgggttga
    25141 tttccccttc gtcactctgc atttttaccc agttcgcggc agttaccagc tgtttagcca
    25201 gtttttcgtt gaggatcagc tcaccttgcg cgctaacatc tggctcatag cgcagggtac
    25261 attccacttt accgcgcgtc aggcgagaac gaatacgctc gcgaacgaca ggttcaaggc
    25321 tacggaactg ctccggcaga cgaaagtaag tttccagata acgctggttt accgagcgca
    25381 tttcccaggt tgcgctcccc cattcaccct tgatttcacg ccgggcgtag gcggtcatac
    25441 tgcggatcat agacgttcct gtttataaaa ggagaggtgg aaggattata gccatcgatg
    25501 ccttgtcagg ataggaataa ccgccggaag tccgtataat gcgcagccac atttgtttca
    25561 agccggagat ttcaatatgc gtccagcagg ccgtagcaat aatcaggtgc gtcccgttac
    25621 cctgactcgt aactatacaa aacatgcaga aggctcggtg ctggtcgaat ttggcgatac
    25681 caaagtgttg tgtaccgcct ctattgaaga aggcgtgccg cgcttcctga aaggtcaggg
    25741 ccagggctgg atcaccgcag agtacggcat gctgccacgt tctacccaca cccgtaacgc
    25801 tcgtgaagcg gcgaaaggta agcagggtgg acgcacaatg gaaatccagc gtctgatcgc
    25861 ccgtgctctt cgcgcggcag tagatttgaa agcgctgggt gagttcacca ttacgctgga
    25921 ctgcgacgtg cttcaggctg atggtggcac gcgtaccgcg tcgattacgg gtgcctgcgt
    25981 ggcgctggca gatgcgctac agaagctggt ggaaaacggc aagctgaaaa ccaacccgat
    26041 gaaagggatg gtagccgcag tttctgtcgg aattgtgaac ggcgaagcgg tttgcgatct
    26101 ggaatacgtt gaagactctg ccgcagagac cgacatgaac gtagtgatga ccgaagacgg
    26161 gcgcatcatt gaagtgcagg ggacggcaga aggcgagccg ttcacccatg aagagctact
    26221 caccttgttg gctctggccc gagggggaat cgaatccatt gtagcgacgc agaaggcggc
    26281 gctggcaaac tgatttttaa ggcgactgat gagtcgcctt ttttttgtct gtagaaaagt
    26341 aagatgagga gcgaaggcat gaaaccatat cagcgccagt ttattgaatt tgcgcttggc
    26401 aagcaggtgt taaagtttgg cgagtttacg ttgaagtccg ggcgcaaaag cccctatttc
    26461 ttcaacgccg ggctgtttaa taccgggcgc gatctggcac tgttaggccg tttttacgct
    26521 gaagcgttgg tggattccgg ccttgagttc gatctgctgt ttggccctgc ttacaaaggg
    26581 atcccgattg ccaccactac cgccgtggcg ctggcggagc atcatgacct tgacctgccg
    26641 tactgcttta accgcaaaga ggcaaaagac cacggtgaag gcggcaatct ggttggtagc
    26701 gcgttacaag gacgcgtaat gctggtagat gatgtgatca ccgccggaac ggcgattcgc
    26761 gaatcgatgg agattattca ggctaatggc gcgacgcttg ctggcgtgtt gatttcgctt
    26821 gatcgtcagg aacgggggcg cggcgagatt tcggcgattc aggaagttga gcgtgattac
    26881 aactgcaaag tgatctctat catcaccctg aaagacctga ttgcttacct ggaagagaag
    26941 ccggaaatgg cggaacatct ggcggcggtt aaggcctatc gcgaagagtt tggcgtttaa
    27001 agaaactcgc cggatgaaaa gtcatccggc gtcatattac tgcaactgtg ccgcaattag
    27061 cggccagcgg gcgtcaaaat catccgtcgg gcggtattta aattcgctgc ggacaaaacg
    27121 tgacagcata ccttcacaga aggctaggat ctggcttgcc agcagggttt catcggtggt
    27181 gtaaccttca ccctcacgca ttctcttttc acgcaatacc tgacgcagct gcgcttcaat
    27241 acgctcgaac agctggttga tgcgcccttg caggcgatcc tgttcaaaca ttagcgcatg
    27301 accagtgagg atgcgggtca ggccaggatt acgctcacca aaaccgagaa gcagcaacac
    27361 aatcagacgc aggcgcgctg tggtgtcttt ctcatctttc agaatcagat tgatgcgagt
    27421 aatcaggcta tcttcgataa actcaatcag gctatcgaac atgcgggtct tactggggaa
    27481 gtggcgatac agtgccgctt cggaaacgcc gacagaggcg gccagttttg ccgtcgtgat
    27541 acgttggctt ccatcgctgg attccagcat cagcgccaga gactgaagta tttcctcgcg
    27601 acggttcctt ttcgcagttt gtttttctgc catgttacaa aatacccctg aaaataagca
    27661 cttgccaggc ggcacccacg ctatgaccgc aaacgaaatg tttacggcta tgttatgacg
    27721 ttattcggat gcgtatgtgt tactgacgac cagagtgacc aaagccgcct tcaccgcggt
    27781 cggtggcgtc gaaatcttcc accagattaa attcagcctg tactaccgga acaaaaatca
    27841 tctgggcgat acgttcgcca ggttgaatgg tgaagctgtc ctgaccacgg ttccacacgg
    27901 aaatcatcaa ctggccctga tagtcggaat cgatcaatcc taccaggtta ccaagcacga
    27961 taccgtgctt atgtcccaat ccggagcgcg gcagcatcat tgccgccagt gaaggatcgg
    28021 caatatgaat cgccagcccg gtcggaacca gcgtagtgtc acccggagcc agttctacgg
    28081 cgtcgtcgag acaggcacgc aggtcaagtc cggcagagcc agaggtggca taagtcggga
    28141 gcggaaattc cttcccaacg cgcgggtcca gaatcttaac gtcgattttt ttcatcataa
    28201 cgggtcacga tctcgtcgag taataattgg ccaaggagct ctttgcgctc aagcggtaag
    28261 actttatctc cgtcctgcca gaaaaggtgt aatgcgttgt tgtcgctgtt aaatccttga
    28321 gttggctggg aaacatcgtt cgcgcagatc agatcaaggt ttttacggat acgtttttgc
    28381 cgggcgtatt cttccacatt atttgtttcg gcggcaaatc caacgacgta gggtcgatgg
    28441 tcttttagtg cggcaacgcc tgcgacgata tcggggtttt taaccatttt tattgttaat
    28501 tcatcaccct gcgtggcctg ctttttgatt ttctctgggg ccacggtagc tgcgcgataa
    28561 tccgccacgg cggcgcagcc gataaaaata ttttgctgct gtacagaagc attcacggcg
    28621 gcttccattt ccagcgcggt catcacatca acacgtttaa caaacggtgg cgtcggtagt
    28681 gaaaccggac ctgataccag cgtgacgttc gcgccacgac gggcagcggc ggcggcgata
    28741 gcaaaaccca tcttgccgga gctgtgatta gagatataac gcacgggatc gagcggttca
    28801 cgcgtcgggc cggcggtaat cataatgttc agatgtttca ggtcgttgac gggcgaaaaa
    28861 tgcgctaccg ccatatccac aatggttaac ggatcgagca ttcgcccagg accgatatca
    28921 ccacaagcct gactgccact gtctggcccc cagatgagca aaccacggga agcaagcacc
    28981 tctaaattat gctgcgtggc agcggcacgg tacatctgct ggttcatggc ggggagcacg
    29041 gctacaggcg caggtgtagc cagacaaatc gtcgatacca ggtcattcgc cattccggca
    29101 gcaacacggg caatcaaatc tgccgtggca ggggcgagaa tcactaaatc agcccattta
    29161 cccagctcaa tatggcccat agcggcttcg gctgccgggt ccagcaaact gtcggaaacg
    29221 ggataaccag aaaccgcctg caagctaagt ggggtgataa aggcttttgc cgcttcggtc
    29281 atggctacgc ggacgtcggc cccgcgatcg cgcaaacgac gcaccagttc aggggtttta
    29341 taggcagcaa taccgccgct aacgccgaga acgatttttt taccggccag gctcatgatg
    29401 atttttcctg ttgggtgaca ccagaagttg gcgattttat cacaatactt ttgttgtcgt
    29461 gccttcacct gagattcact ttgcgaggcg ctttccagga ttgaaaactg gccgtcgatt
    29521 taacggaacg gctatgacag gatgcgagca ccacaaagga ggtgaaggtg aaaaacaatg
    29581 cacagctgtt gatgccgcgc gaaaaaatgc tgaagtttgg tattagcgcc ttaactgatg
    29641 tcgagctgct ggcgctattt ctgcgtaccg gaacgcgcgg taaagatgta ttaaccctgg
    29701 caaaagagat gctggagaat ttcggctctc tttatggctt gttaacctct gaatatgaac
    29761 aatttagtgg tgttcatgga attggcgtag cgaaatttgc ccagttaaag gggattgctg
    29821 aactggcgcg acgttactac aacgtgcgga tgcgtgaaga aagcccttta ctcagcccgg
    29881 agatgacgcg ggaattttta caaagccagc tcacgggtga ggagcgggag atctttatgg
    29941 tgatctttct cgactcccaa caccgggtta taacgcatag ccgtcttttt tccggcacgc
    30001 taaaccatgt tgaagtccat cctcgggaaa ttatccgcga agcgataaaa ataaacgcct
    30061 cggcgctgat ccttgcacat aatcaccctt cgggttgtgc tgaacccagt aaagcggata
    30121 aactcattac tgaacggata ataaagagtt gtcagttcat ggatttacgc gtgctcgacc
    30181 atatcgtgat tgggcgtgga gagtatgttt cttttgccga acgcggctgg atttaacccg
    30241 ctatgcgcga tccttcggga tctttgtctg ttcgggactt gagcacatcg ctgagtcagc
    30301 gtatactacg ccacctttga gaatctcggg tttggcattt gggcctggca atcgagagtt
    30361 cacatagaac tgcgatgacc gggctgtaaa gcctgacgag gcgccaatac cccatacgaa
    30421 gctcgagcta atttgatttt tggagaatag acatgtcccg agtctgccaa gttactggca
    30481 agcgtccggt gaccggtaac aaccgttccc acgcactgaa cgcgactaaa cgccgtttcc
    30541 tgccgaacct gcactctcac cgtttctggg ttgagagcga gaagcgtttt gtcaccctgc
    30601 gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg catcgataca gttctggctg
    30661 aactgcgtgc ccgtggcgaa aagtactaag tacttagagg aaataaatca tggctaaagg
    30721 tattcgtgag aaaatcaagc tggtttcttc tgctggtact ggtcacttct ataccactac
    30781 gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa aaattcgatc cagttgttcg
    30841 ccagcacgtg atctacaaag aagcgaaaat caaataattc tcgctttgat gtaacaaaaa
    30901 acctcgctcc ggcggggttt tttgttatct gcttgccccc atattgactg catctgttca
    30961 ttcctggaga tgctatgcct gaattacccg aagttgaaac cagccgccgc ggcatagaac
    31021 cgcatctcgt tggtgcaacc attcttcatg cagtggtgcg caacggacgc ttgcgctggc
    31081 cggtttcaga agagatctac cgtttaagcg accaaccagt gcttagcgtg cagcggcggg
    31141 ctaaatatct gctgctggag ctgcctgagg gctggattat cattcattta gggatgtctg
    31201 gcagcctgcg catccttcca gaagaacttc cccctgaaaa gcatgaccat gtggatttgg
    31261 tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg ccgctttggt gcctggctgt
    31321 ggaccaaaga gctggaaggg cataatgtgc tggcccatct tggaccggag ccgcttagcg
    31381 acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa gaaaaaaacg gcgattaaac
    31441 cgtggctgat ggataacaag ctggtggtag gggtagggaa tatctatgcc agcgaatcac
    31501 tgtttgcggc ggggatccat ccggatcggc tggcgtcatc actgtcgctg gcagagtgtg
    31561 aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc gattgagcag ggtggtacaa
    31621 cgctgaaaga ttttctgcaa agtgatggta aaccgggcta tttcgctcag gaattgcagg
    31681 tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac gccgattgtg gcgactaaac
    31741 atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa gtaattcatg cgcgccggat
    31801 ggcataccat ccggcataaa cgctacgcta acttcgccat cagcgcctga tggacattct
    31861 ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac ctctttcacc aacgatgaag
    31921 agataaacga ccactctttc gacggcatca gaaacacact ttccagttcc ggcattaagt
    31981 ggcgattcat atgcgccagc tgcatttcat attcaaaatc tgccaccgca cgcaggccac
    32041 gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc catcaaatca ctaaacccga
    32101 ccacttccac gttccccaga tgcgcggttg cctgctgtgc cagtgccaca cgctcttcca
    32161 gggtaaacat cggtttttta ctggggctgg cggcaatcgc cagaataacg tgatcgaaca
    32221 tctgcgtggc gcgcgtcacg atatcgatat gaccattggt aatgggatcg aaagtacccg
    32281 gataaatcgc ccgtttttgc ataacaacct caatgcgttt tcggtggcag gtaaggttcc
    32341 agcagttgaa gcagacgctg tagcgcgccc tggttttgat acagtacttc aacggcatga
    32401 cggccataga aactacggta atcggcgtcg gtgagtaaag aggaaacctc ttttgcaagc
    32461 gtagtggcat cggtaacggt aatcagcccg cttgcctgct ccagccgcgc gcaaatgtct
    32521 ttaaagttaa aagtatgcgg ccccatcaat accggaattg cgtgtgcggc agcttccagc
    32581 ggattatgcc caccacgttc aaccagtgaa ccgccaacaa aggcgagatc ggcaatgccg
    32641 tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa cctgcgtgct ggtggagggg
    32701 acttcccctg aagagcgtgt gatatagctt agtccagcct ggcggacaag gttaatcgca
    32761 tccgggaagc gttctggatg acggggtacc aggatgagca ataaattcgg gaattgctgt
    32821 aataaagcct gatgtgcggc aatgaccacg ctctcttcac cttcgtgagt gctggtggca
    32881 atccataccg ggcggtgtgg tgcccactgg cggcgcagcg tcacggcttt agcagccaac
    32941 tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa cggttacctg gttatttttc
    33001 gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt gggcggcaat cagcgtaata
    33061 cgacgcagca agcgacggac gaatttaccc agtttggcat aacctgcggc cgagcgggca
    33121 gagagtcgcg cgttagcgat caccagcgga attttacgtt tatgtagcgc cgcaatcagg
    33181 ttaggccata gttcggtttc cataatcaac accagtttag ggtcgacttt attcaggaaa
    33241 cggttgagtg catcgggcag atcatacggc agataaacgt gctgaacatc cttcccgaaa
    33301 gccgattgta cgcgctccga accggttggc gtcatggttg ttacggtaat aggtaaatca
    33361 ggataacgat gacgcagcgc gcgcaccaac gggatcgccg ccagagtttc accgacggag
    33421 acggagtgca gcataatgcc gcctggtttt agcggatggc ggtaaaaacc gtaacgttca
    33481 ccccagcgtt ttcgataggc cggagcctta cgtccgcgca cccagagccg tatccagatc
    33541 agcggctgaa taaggtagag aagggcggtg taaagcaatt cgagcatagt aaatagctga
    33601 cttatggatg tgctggggat tctatgtatt tagctgtggc tttaccatta cttttcccgt
    33661 ttttgactta aatagcaatt tacccgccaa ttcttgctga acatctatac tttttttgta
    33721 tttttatgcg attcattgat ttctgctgta cgtgcaggaa ggaatcgcag agcaacagtg
    33781 aaagaatatg gttgctaatg attttatttg attgatttct ttattgaaaa tcaacaatgt
    33841 gcttaaaaat cattcgggtc ggattgccgc gaaagaagtg atacactagc gcgtcaatat
    33901 cagtgcggta tcagtattca ggtagctgtt gagcctgggg cggtagcgtg ctttttctgc
    33961 ttcaccttaa tcggataatc tcaacaaaaa gagttacttg tggataagcc atttcgaaga
    34021 attttgctca ttaagatgcg ttttcatggg gatatgttat taactactcc cgtcattagt
    34081 tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc tgctttatca ggacaccatc
    34141 ccgatcctgt ctgaaaatcc agagattaac gcgctctacg gcataaaaaa taaaaaagca
    34201 aaagcctcag aaaaaattgc caactttttt catctcatca aggtattacg tgccaataag
    34261 tatgacctta tcgtcaatct taccgatcaa tggatggttg ctatactggt tcgcttatta
    34321 aatgcccgtg tgaaaatttc ccaggattat catcatcggc agtctgcttt ttggcgtaaa
    34381 agtttcaccc atttggtgcc gttgcagggt ggaaatgtgg tggaaagtaa cttatccgtg
    34441 ctgacaccat tgggacttga ttcgttggtg aagcagacaa ccatgagtta cccgcctgca
    34501 agctggaaac gtatgcgtcg cgaacttgat cacgctggtg ttggacaaaa ttatgtggtt
    34561 atccaaccta cggcgcggca aatcttcaaa tgctgggaca acgccaagtt ttccgctgtg
    34621 attgatgcct tacatgctcg tggttatgaa gtcgttctga cgtccggccc agataaagac
    34681 gatctggcct gcgtcaatga aattgcgcaa ggatgccaga cgccaccagt aacggcgctg
    34741 gctggaaagg tgaccttccc ggaacttggt gcgttaatcg atcatgcgca gctgtttatt
    34801 ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta atacgccgct gatatcgctg
    34861 tttggtgcga cagaccatat tttctggcgt ccttggtcaa ataacatgat tcaattctgg
    34921 gcgggagatt accgggaaat gccaacgcgc gatcagcgtg accgaaatga gatgtatctt
    34981 tcggttattc cggcggcaga tgtcattgct gctgtcgata aattactgcc ctcctccacg
    35041 acaggtacgt cgttatgatc gttgcttttt gtttatataa atattttccc tttggcggtt
    35101 tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc ccgaggtcat catgttcggg
    35161 tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt tgaactgatc aaagtgccgg
    35221 ttaaatcgca taccaatcac gggcgcaatg cggagtattt tgcctgggtg caaaaacatt
    35281 tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa aatgccgggg ctggacgttt
    35341 attatgccgc tgatgtttgt tatgccgaga aagtcgcgca ggaaaaaggc tttttctatc
    35401 acctgacgtc acgttatcgc cattatgccg cctttgagcg ggcaaccttc gaacagggca
    35461 agccgacaca gctgctgatg ctgacagata agcaaatcgc cgatttccag aaacattatc
    35521 agactgaagc ggagcgtttt catattctac caccggggat ttatcctgat cgtaaatata
    35581 gccagcagcc agctaatagc cgtgaaatct tccgtaagaa gaatggaata accgaacaac
    35641 aatatttatt gttgcaggtc ggttcagact tcacgcgtaa aggtgtcgat cgttccattg
    35701 aagcacttgc ttcgttaccg gattcgctgc gccacaacac attgctatat gttgttggac
    35761 aggataaacc gcgaaaattt gaggcgctgg cagaaaaacg cggcgtgcgc agtaatgttc
    35821 acttcttctc ggggcgcaac gatgtctcgg aattaatggc ggcggcggat ttattactgc
    35881 atcctgccta ccaggaagca gcgggaattg tgctactgga agcgattact gcaggattac
    35941 cggtactaac aacagccgtt tgtggctatg cgcattatat tgtcgacgcc aattgcggcg
    36001 aggctattgc tgagccattc cgccaggaaa cattgaatga gattttacgc aaagcgttaa
    36061 cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg acattatgct gatacacaag
    36121 atttatacag tctgccagag aaagcggcgg atatcataac gggtggtctg gatggttgaa
    36181 cttaaagagc cgtttgccac gttatggcgc ggtaaagatc cttttgagga agttaaaacc
    36241 ttgcagggtg aggtttttcg tgaactggaa actcgccgca ctctgcgatt tgaaatggcg
    36301 ggcaaaagct attttctcaa atggcatcgc ggcacgaccc tgaaagagat aatcaaaaat
    36361 ttactctcat tgcggatgcc agtattaggc gcagaccgcg aatggaatgc gattcatcga
    36421 ctgcgggatg tcggcgttga tactatgtat ggggtggcat ttggcgaaaa aggcatgaat
    36481 ccgctgacca gaacttcatt tattattacc gaagatctga caccaaccat aagtcttgaa
    36541 gattactgtg ctgactgggc gactaaccct ccagatgttc gcgtaaagcg tatgcttatt
    36601 aagcgtgtcg cgacgatggt gcacgatatg catgctgcgg gcattaacca ccgtgactgt
    36661 tatatctgtc atttcctgct gcacttgcct ttttccggta aggaagagga gttaaaaatt
    36721 tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg ttccacgtcg ttggcgggat
    36781 aaagatctta ttgggcttta tttttcttcg atgaatatcg gcctgactca gcgggatatc
    36841 tggcggttta tgaaagtgta ttttgccgcc ccgcttaaag acattctcaa gcaggaacaa
    36901 ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca gggaaagaac gattcgaaaa
    36961 tcgttgtaac ccgtaactta tttttgccaa aattttggat acagaataaa tatgtctcaa
    37021 ctcaatgata gtgacatcat cctttttgag tataattttc attatcaaaa tataagatct
    37081 aaaaatactc ttgatatagc atttggtatt gacagaaatt ttctt
//
LOCUS       DAZFTN010000043        36182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFTN010000043 DAZFTN010000000
VERSION     DAZFTN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(386..715)
                     /locus_tag="ACHKV2_002287"
     CDS             complement(386..715)
                     /locus_tag="ACHKV2_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940164.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIESFETAYKGSPEEK
                     HEFTSYY"
     gene            1313..1978
                     /locus_tag="ACHKV2_002288"
     CDS             1313..1978
                     /locus_tag="ACHKV2_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO6940165.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
     gene            2052..2336
                     /locus_tag="ACHKV2_002289"
     CDS             2052..2336
                     /locus_tag="ACHKV2_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6940166.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(2354..2950)
                     /locus_tag="ACHKV2_002290"
     CDS             complement(2354..2950)
                     /locus_tag="ACHKV2_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6940167.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(3100..3222)
                     /locus_tag="ACHKV2_002291"
     CDS             complement(3100..3222)
                     /locus_tag="ACHKV2_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6940168.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGDREVTASLAYESNGK"
     gene            complement(3167..3319)
                     /locus_tag="ACHKV2_002292"
                     /pseudo
     CDS             complement(3167..3319)
                     /locus_tag="ACHKV2_002292"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3455..3616)
                     /locus_tag="ACHKV2_002293"
     CDS             complement(3455..3616)
                     /locus_tag="ACHKV2_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940169.1"
                     /translation="MPLIEYINKFYRGNQASFARLTGVQPAQVTQWINKGFIVVNHTL
                     YSPRRKLGI"
     gene            complement(4665..5693)
                     /gene="repA"
                     /locus_tag="ACHKV2_002294"
     CDS             complement(4665..5693)
                     /gene="repA"
                     /locus_tag="ACHKV2_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO6940170.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDSYKALGGQLGHAYNRLVKKDPVTGQYTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGNVIPETAVTVSRLSRLLAEQVCFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKFRR
                     EKAAKRKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            6777..7283
                     /locus_tag="ACHKV2_002295"
     CDS             6777..7283
                     /locus_tag="ACHKV2_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFO6940171.1"
                     /translation="MTLYSGDVSISDKQPGENMARKLKINPVIPECVADLKGYPLYII
                     VAYWGLRNNKTLTTTDVSRNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRI
                     SAFRILDIDKGILRTPKKEIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGD
                     RVEVFLNV"
     gene            7276..7491
                     /locus_tag="ACHKV2_002296"
     CDS             7276..7491
                     /locus_tag="ACHKV2_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO6940172.1"
                     /translation="MSKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIPPQNAGNTYG"
     gene            7484..7660
                     /locus_tag="ACHKV2_002297"
     CDS             7484..7660
                     /locus_tag="ACHKV2_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940173.1"
                     /translation="MADFGSTKQTVTFEEWHELLMDYAELRGGNAADAEAWRGDYEAG
                     KTPVEAYCDEWGED"
     gene            7687..8640
                     /locus_tag="ACHKV2_002298"
     CDS             7687..8640
                     /locus_tag="ACHKV2_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6940174.1"
                     /translation="MVTSIPVLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDVPKQEVISKDNANVTIDAVCFIQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPEELTKAMNAQMKAERTKRAQILEAEGIRQSQILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTSKVDMKQAQQYLS"
     gene            8702..9145
                     /locus_tag="ACHKV2_002299"
     CDS             8702..9145
                     /locus_tag="ACHKV2_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6940175.1"
                     /translation="MFWFTLFLLCIALEIITGTGWLLVISLGALSSAIVDFFLPISQE
                     ANICFFASISILTSIIKFVYDKKHKKLDTPLVNTGHSRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHNENYPAGTRVIVTDMQGITLKVMTIEDKNENKE"
     gene            9149..9319
                     /locus_tag="ACHKV2_002300"
     CDS             9149..9319
                     /locus_tag="ACHKV2_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940176.1"
                     /translation="MHLSLLIPLTGAAMNALCIFIIRLLDKDFIEAYKKTLKNITALH
                     ILLMVLSVTGIV"
     gene            9330..10025
                     /locus_tag="ACHKV2_002301"
     CDS             9330..10025
                     /locus_tag="ACHKV2_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940177.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKVLVFGVLFGLSARLFTFLSDTVSDF
                     AEPVTTTTATGKIQSILDGMAEMVSILGTVVPFLVIILGTVIPFLVIIIAMIWFLFFS
                     SKREKEELTRVEREAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIE
                     ALVDESNDISKRN"
     gene            10209..10424
                     /locus_tag="ACHKV2_002302"
     CDS             10209..10424
                     /locus_tag="ACHKV2_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940178.1"
                     /translation="MSAFIKLASVLALFISPVVVAIAGEQSHCTKINEYPFIVTQCDD
                     GTVTVVNVINNRVSVCRKGESCKEIKL"
     gene            10440..10742
                     /locus_tag="ACHKV2_002303"
     CDS             10440..10742
                     /locus_tag="ACHKV2_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940179.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKSIANTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSSTSKIRTTL"
     gene            10739..10996
                     /locus_tag="ACHKV2_002304"
     CDS             10739..10996
                     /locus_tag="ACHKV2_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940180.1"
                     /translation="MKKNDDLITLPSHNSHALDYIIKKAFTPRKTKTSTATHNVYSYN
                     KDESTLNFQKIGEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            11000..11995
                     /locus_tag="ACHKV2_002305"
     CDS             11000..11995
                     /locus_tag="ACHKV2_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO6940181.1"
                     /translation="MNIVSEFLEKVTTIVESTAATNAAKVAQAISPVFFAAIALYVIY
                     LIYEITYAQRDILMNEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATSVDNLWNALSTNLNNFLNTSMSIYGKFQIAEKMGVYLVWGIGYIGGMLLIYY
                     TTVFLTLSTFMVGILLSAGILFICFALFSSTRNMFTAWVGSCLNYILLNLFYSISFSF
                     LISFVESVVPVNGDIKLSIVVYFFLITIISIFLIEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPIFNAGSRSASALVQGLNRGSKLKAG"
     gene            12001..12645
                     /locus_tag="ACHKV2_002306"
     CDS             12001..12645
                     /locus_tag="ACHKV2_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO6940182.1"
                     /translation="MKDKVLSFLFAGIITVTSPSIFASGIPVVDVTAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNIAETQQKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLIASENARQEAVSREKFKLMMQ"
     gene            12654..12878
                     /locus_tag="ACHKV2_002307"
     CDS             12654..12878
                     /locus_tag="ACHKV2_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO6940183.1"
                     /translation="MKKFISTLTILSCFLLAACEDKVYDVSYYAEHLEQAQDVVEKCS
                     KGDMSGQNCENAREAIQKEQSGKAFKNMMQ"
     gene            complement(13101..13910)
                     /locus_tag="ACHKV2_002308"
     CDS             complement(13101..13910)
                     /locus_tag="ACHKV2_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFO6940184.1"
                     /translation="MNEPLEAELHSLFSFDIYPGASSEQNTGVKLAMARFYLNVPFEE
                     KDLAKQKGAQWDQEQRKWFVPQGKNPIYFIRWIKELNEHDYNVFSQRFYIAESYQSCW
                     RCKKTTPVFGIFLPRWYKYRDVIWGVDPAEWEDCILDEWYETSSPKGMEYFDSKKNMI
                     YRWLTSRVWWTDLTKIEIISTSALSRINEYSKLYYPSHSKTAKMNYYANHCCHCNAMQ
                     GDFMMFNEPGGVFFPVTYEQAEKIRFHEVNETIFAKASYSLIPEAGGFIDL"
     gene            13958..14593
                     /locus_tag="ACHKV2_002309"
     CDS             13958..14593
                     /locus_tag="ACHKV2_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940185.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSEKIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIERGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            14660..14959
                     /locus_tag="ACHKV2_002310"
     CDS             14660..14959
                     /locus_tag="ACHKV2_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO6940186.1"
                     /translation="MKSIILSMVAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSE
                     CKSAEKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            14962..16197
                     /locus_tag="ACHKV2_002311"
     CDS             14962..16197
                     /locus_tag="ACHKV2_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO6940187.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPALPPAKPISSNNHQAQTAQKTDQQT
                     KPRPEQPPLMSTSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKAVKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKPLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGTSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            16203..16640
                     /gene="pilM"
                     /locus_tag="ACHKV2_002312"
     CDS             16203..16640
                     /gene="pilM"
                     /locus_tag="ACHKV2_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO6940188.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFIH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            16759..17157
                     /locus_tag="ACHKV2_002313"
     CDS             16759..17157
                     /locus_tag="ACHKV2_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940189.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADVIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            17178..17762
                     /locus_tag="ACHKV2_002314"
     CDS             17178..17762
                     /locus_tag="ACHKV2_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6940190.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEITPPNDEEKTSPDWDVFGDFS"
     gene            17762..18052
                     /locus_tag="ACHKV2_002315"
     CDS             17762..18052
                     /locus_tag="ACHKV2_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6940191.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFS
                     FGGVCALCYLIYNVIMAFMEKKSWSDVGMALVYCSLAGGALYAGNWALELFK"
     gene            18123..18443
                     /locus_tag="ACHKV2_002316"
     CDS             18123..18443
                     /locus_tag="ACHKV2_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO6940192.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALVAGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            18449..20806
                     /locus_tag="ACHKV2_002317"
     CDS             18449..20806
                     /locus_tag="ACHKV2_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO6940193.1"
                     /translation="MHKVKLKNSDNMDVAREYPDYRFHVTDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRATINGEWNFDNDFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALLPFNASLLSV
                     YNTYISEVADYLSLLLNAEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTDSGSVYWFNTHYSPPEKNVTGQKIAGHAMFLGATGTGKTTFEATASGFLQRF
                     DPLMFVVDYNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAEGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFFKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQDMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            20969..21703
                     /locus_tag="ACHKV2_002318"
     CDS             20969..21703
                     /locus_tag="ACHKV2_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO6940194.1"
                     /translation="MENTNYYEHSKKEASKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            21769..22470
                     /locus_tag="ACHKV2_002319"
     CDS             21769..22470
                     /locus_tag="ACHKV2_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO6940195.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTERPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            22460..23599
                     /locus_tag="ACHKV2_002320"
     CDS             22460..23599
                     /locus_tag="ACHKV2_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO6940196.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQADNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            23618..24673
                     /gene="virB11"
                     /locus_tag="ACHKV2_002321"
     CDS             23618..24673
                     /gene="virB11"
                     /locus_tag="ACHKV2_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO6940197.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            24689..26647
                     /locus_tag="ACHKV2_002322"
     CDS             24689..26647
                     /locus_tag="ACHKV2_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO6940198.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            26694..27230
                     /locus_tag="ACHKV2_002323"
     CDS             26694..27230
                     /locus_tag="ACHKV2_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6940199.1"
                     /translation="MRIKHSNMINMNMNMNMNMMKEAIDVLINSEKHILIIGETGTGK
                     STLLAELLINDTDVKYFDFCDIYKRHEHLPLLKHHYLCDENFDYFDFLSAPEKTLVLN
                     SVAIGNNLRESKVVQFIVRFIKTARKRGKRLIVVTTPSDGGVQIQNLFDTVISLTRSH
                     DEIGCTVIIPVPELIKYE"
     gene            27223..28866
                     /locus_tag="ACHKV2_002324"
     CDS             27223..28866
                     /locus_tag="ACHKV2_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO6940200.1"
                     /translation="MNKKLLCLLISSFLLSGCANFKKINANELAAERDAQKATEYAKK
                     IRNNPVIQDSTKQWINTTPIIEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIP
                     VRISADVWTYLNGTGTGSTQQMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELN
                     ISGLPALLQTVSSRLGISWKYDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSS
                     SGSTGSTSSGGMTGDASNTQTTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTV
                     TDTPDVLDSVQEIVNKRNNEMSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLS
                     LGGSFGNAAENVITSGVSIVDGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPM
                     QIANQQGFIESVTTDSTANVGSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSL
                     SDKPTFEVFESGGSKAQTPNVDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITP
                     SFFGLGGGINSEDGDTILVVLITPNVLNSGSSAVIKNVN"
     gene            28917..30227
                     /gene="pilO2"
                     /locus_tag="ACHKV2_002325"
     CDS             28917..30227
                     /gene="pilO2"
                     /locus_tag="ACHKV2_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO6940201.1"
                     /translation="MSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTFARKN
                     DAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAICELG
                     DLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPEPGWKLYQPESWDISQALPS
                     LTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKEAAAE
                     AARLRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLAECST
                     SGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSFDVSP
                     DPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYELMIQ
                     TSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            30211..30705
                     /gene="pilP"
                     /locus_tag="ACHKV2_002326"
     CDS             30211..30705
                     /gene="pilP"
                     /locus_tag="ACHKV2_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO6940202.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            30730..32268
                     /locus_tag="ACHKV2_002327"
     CDS             30730..32268
                     /locus_tag="ACHKV2_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO6940203.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVHVYIADNSKGQRAVQ
                     AYIGTLNVKYPGKVNITWTTLDVIASRYQDSERRSDSNSSQHLNSNQEKVISYLAKAN
                     NLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            32259..33368
                     /locus_tag="ACHKV2_002328"
     CDS             32259..33368
                     /locus_tag="ACHKV2_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO6940204.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            33413..33970
                     /locus_tag="ACHKV2_002329"
     CDS             33413..33970
                     /locus_tag="ACHKV2_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6940205.1"
                     /translation="MSSINILNMRSVFSSLSARRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEANVPQKNCMAMVNALRSSSAISKINN
                     TSTSTVSAATVCASDSNTLTFSTDS"
     gene            34036..34518
                     /locus_tag="ACHKV2_002330"
     CDS             34036..34518
                     /locus_tag="ACHKV2_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6940206.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            34522..35157
                     /locus_tag="ACHKV2_002331"
     CDS             34522..35157
                     /locus_tag="ACHKV2_002331"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO6940207.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            35170..>36182
                     /gene="pilV"
                     /locus_tag="ACHKV2_002332"
     CDS             35170..>36182
                     /gene="pilV"
                     /locus_tag="ACHKV2_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO6940208.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGTSCSPNGLVGRD
                     STGA"
BASE COUNT        10948 a         7912 c         7635 g         9687 t
ORIGIN      
        1 agcggggcca catcgccccg aagcgcggcg cagccgctga cgaacatacc aggatgcaca
       61 gggccatccc ttcgccctgc aaagccagga cagaccaaca ggccatgata atgctgattt
      121 gcgccgcaga atgccgcagt tagcgcgagc gccagcgagt ttacgccgtc ctggtgcatt
      181 gcagcagggc ggggcgctgt actgcgcgtg tgagcgtcag cgagctacgg gcgcaacatt
      241 acattgctgc attcgcctgg cattctccac gccttgaagg acggagtttt actgcgcacc
      301 agataaaaaa aggggcgatt aagcccctgt attcactatt aacaatttaa catggttgca
      361 ggggataccc cctgcaacta ttttcttagt agtatgatgt gaactcgtgt ttttcttccg
      421 gtgatccttt atatgctgtt tcgaatgact caatttcaat tgctggcgta ctgtaataat
      481 cacgcactcg ccgttttaat ggctcaccac agaaatcatt gagtaatggg gtcttatatc
      541 cgctcattga atgagtccga tgaactgaag cgcgaatctc ctggacagtt acggtttttt
      601 tgccatgaac agaaataacc tgatagaaat taacgttcgt ttgctcatat ccccaggaag
      661 atacaaacac atcgccaaca gaaacagtgc cgttattaaa agttaatgtt ttcattttta
      721 tctcctgccg ctgagcggct ttggtggttg gggaccatcc cctcccgatg tgtttataat
      781 aggtcagaac tgacctatta tcaagtattt ttccctccat tcttcaaaaa aaacaccatc
      841 aaatacatat agttacattc atgtgatgcc cgacagggca tggaaggggc gaaagaaaat
      901 accagggagc agcggggcca catcgccccg aagcgcggcg cagccgctga cgaacatacc
      961 aggatgcaca gggccatccc ttcaccctgc aaagcccaac ggactgacag gccatgatca
     1021 tgctgatttg cgccgcagaa tgccgcagtt agcgcgagcg ccagcgagtt tacgccgtcc
     1081 tggtgcattg cagcagggcg gggcgctgta ctgcgcgtgt gagcgtcagc gagctacggg
     1141 cgcaacaaca caccaggcaa aatcgcatcc tgaaaagtta ccctgtaaca tacacacaat
     1201 gtaaagcgca aaaaacaatt acattgtaaa gacacaaaca tctttacaac gtaactataa
     1261 tgaatacaat gtaaagacaa ataaaccttt acactgtata tccgaggttt ctatggggag
     1321 agcattactt gtcgcatcag ataaaggcgg tgtaggaaaa agcaccacag caacaaacct
     1381 ggcttcttat ctggtaaata attcacgaac ggtcatcatt ctgaagaccg ataaaaacaa
     1441 tgatattctt agctggaacg aaaaaagaaa gcagaatggc ttattaccgg ttcctgttta
     1501 tgaagagtat ggcgatgtat cagaagtcat taagcgcctg aaaaaaatat gtgaagtgct
     1561 gatcattgac tgtccagggc atgacagcaa ggaatttcgc agtgccttaa ctgtagctga
     1621 cattctgctt actctcgtta agccgtcttc agatttcgaa gcagaaacgc tgactcatgt
     1681 aacagaaaaa gttcgcacag ctcaacagat caatccagac ctgcaaccct gggtactttt
     1741 cacgagaatt aacacgacaa aaccacgcca cagaaaagca gcgattgatc tcgacaagtt
     1801 actccgggag aacccggtgt ggatacagcc acttcgtaca cgtatttcag atttagatat
     1861 ttttgaagcg gcctgtaatg aaggcgctgg cgttcatgat gtacgtcgcg catccagcct
     1921 ttctacagct aaagcacaaa tagagctttt ggcgcaagag ttaggcatca atccctaatg
     1981 gatgtaatac gatagcttta catcattaac tttacacagt aaagctaatg taacacacga
     2041 aaggtgatat aatggctctt gacttgaaaa ctccggtaat accaggacaa aaaacagaac
     2101 aaaccaatgc agacacagcc cgattcatca atgaagctac ccgcaaaaaa tcgggagcaa
     2161 ctaaagtgac caatgtccgg cttggggacg tttacgaaga tattctggaa cgggaagcac
     2221 tgcgaacagg ccagacaaaa acagcaataa tgaaagcggc tcttgcagct tacgaacacg
     2281 gcctggacga aaacggaaaa aatcactgga ttctacagtc agccaggctg aaatgataaa
     2341 gcccttcggg gctttatccc ttacgtttta attgtttctt ctcgctggct ttcttacggg
     2401 catcgagaac cagttgcgca taagttgcgt gtgctttttt tactgtacca gcaggtttcc
     2461 cgtaaaggtc gtaacgtgtt gcgcctggta cagtacaatt ctgaagatat tgccaccgga
     2521 acgtcatcac ctgaaccgca caattccact cttcaatcgt cagatccaca tccgggttat
     2581 ccgccaggta tttctcaacg tcctggagaa tcccgctttt caggggcaag ttcttttttt
     2641 cgtctgtcca tagttcagga aaaacagcac gaactttatt gaaacacttt tcaaaatatt
     2701 ctttcggata ttttggggga aaagtgattt gttttttcaa ataccagggc tgaggctgct
     2761 ttttatgtgt tttctctgct acagcatctt tcttctgtac tttcttgttt tcattaactg
     2821 ctgatttttg tacttcctgc ggcttttcat tcaccacagg attttctggc atgaccagaa
     2881 cgcggcgttt tttgaccaca atcacgggtg ttttttttgg ctgcatactc gcctcaataa
     2941 ttgctgtcat attgactaat tataccattt tcagcagtta atatcctgaa aatacatgga
     3001 aaaagggggt atccgtacag aataccccct ttgtaaaggc gcttaatgcc agaaccacat
     3061 catccccaaa aaggcgggct ttcgcccgcc tccgggttgc tacttaccgt tggattcgta
     3121 agccagactt gccgtaacct ccctgtctcc gtctttcagc cggacctcgc acaaacgatt
     3181 ccgggtcaga tacgtgaata ttaacaatgt gcagcacacg attaataaac accaggtcag
     3241 ggcgtttttc ggctgtttca tggccttctt ctccttgcgt aacgcgcggt aagaggctat
     3301 cctgatgttg ttcaagcatt gggatcgcct cgtgggttaa tgaaaaatta actacggggc
     3361 tttcttcttt ctgccgcaca acaagataca aaccaccttt tcgtcatgag gcagaaagcc
     3421 tcaagcgcca acagcattat atattgacca ggaattaaat tcccagcttt cggcgtggac
     3481 tatacagtgt gtgattcacc acgatgaatc ctttgttgat ccactgtgtg acctgcgcag
     3541 gttgaactcc ggtcagcctg gcgaacgatg cctgattccc ccgatagaac ttgttgatgt
     3601 attcaatcaa aggcattttt taattctccg aaacaacgca aaatcatata gtaatttact
     3661 atatgatttt tatttcgcaa gtgattaact catcggattt ctttgtagtt aacgaaacgt
     3721 taaatttagt ttgtgcctgg aatggcgcgc aagaagcaca ctagcattat tattttgatc
     3781 aaaacgctat ttccccccgt cattgggggc tttctttttt gatgccgcgc cagcggcatg
     3841 gaaggcggca ctctgttgtt ttatatggca cagagctaaa ccgccgaagc ccggcgtaag
     3901 ccggtactga ttgataggtt tcaccttacc catccccatc ctgctaagcc atacccgctt
     3961 tcagccatga aatagctttt gtgcgcggtc ggagtggtcc cgacaagggt ttacccgcag
     4021 tcggggcgta tctccgcgtt agcggaccgt cagggccgct tacgagcgtg tatttggtac
     4081 ttccagccag aaggctgaca gcgatgatat ttcttagtta caacatccat tattgaaagc
     4141 gatgttgttc ccgccctttg ggcgggggcg gcggcgcttt gcagtgatgg gttgggcgct
     4201 gttgctgagt atttgcagtg ttttttggag ggttggaggg gctttgtttt tcattgggca
     4261 gaagtccata tgcccgccgc gaagcggtgg gaggtacttt tatattctga gcggcgcggc
     4321 ggtagccgct gcaagcgaag gcatttttgt gattatccac agttgtttca aagataaata
     4381 ttttccctgc ggcgaagcaa cgcaggggat taaagaacag gggttaagaa tatattgcgg
     4441 gtttgatctt ctacgtttgc tgctttctga agttattcgt ttgcgggttt ttgttaataa
     4501 ccgaaggcgg tttagctccg atacccgatt gtggccatca aaaaactgtc agagcgggac
     4561 aaaatgaaaa acggtcgtca aaaatgagga atttcatgaa atgcaggcaa cacgaaaagc
     4621 cccgaagggg ctgaagagag tgccgattgt atcaggcagc taaactagtg gggtggcgaa
     4681 gctgtcaggg ccagttcaag ctgacgtaga tccctggcta ccagttgctc taatctttct
     4741 ttagtgcacc agtacgcttc gtccggcggc atacgtttca gaataaaccg tgcaatttcg
     4801 tttcgctgtt catcatacgg cagttttgca aggcgattgg cacgtttgcg ctttgctgct
     4861 ttctctcgtc ggaacttgat ggcatcgaga gaacgctgcg cataccagcg tttacgagcc
     4921 gagtgaacag agatttcctc gccctcgcgg ataacgcctt ctttaatcag ttgcagacgg
     4981 atttcacttt ctgcaaggcg tttacgttgc tgttcctgaa gtttcaccag atcaactccc
     5041 agcattttcc agccagtttc ggtgatccag acgtatttcg gcagtcgctg gcgggattca
     5101 cgatcccata tcgtcttttc tgacgttccc aacgtgccaa agcaaacctg ctctgccagc
     5161 agacgggaaa ggcgcgatac tgtgactgcg gtttcaggaa taacgtttcc cttactgtct
     5221 tttggactta tttcttctgc caggcgggag acagacatgc cgacagtgtg caatccacga
     5281 tcacaaaaac tcacaagtac aacccatatt gcgtcaagca gccgctgttt ctccggtcgg
     5341 aatttatatt tgcgaccgac actctgacgt ttcagtacat aaaatggatg caacgacatg
     5401 tgcattctga gtgtgtactg accagttacc gggtcttttt taacgagacg attgtaagcg
     5461 tggcccaact gtccgcccag cgccttatag ctgtcgggac ggaaccaggc tggattctca
     5521 cactttggct tagtggaatg ttcaccccgg cgacgttttg ttgttccgcc tttgttttcc
     5581 tgggggacca ggaatgagct ttcaatgccc gcttcgtagt cctgccagaa acggacctgc
     5641 tcttccggtg atagttcaga ccagtgacgt ttgctaatca cagcaagctg cacttagctt
     5701 gcactgtaag agttgatatt tccttctcat ggaattaaac tccatttcag gtaacaagca
     5761 tacctgaaat aaaacgttat cagctcaagg ctcggccttg agggcaaccc ccgctaatct
     5821 acgctgcctg gaaaacacga gattacgggg gttttcttta tctgaaattt acgttcttga
     5881 acaaaatttc agcgcctcgt tttctgcatt tacaaatcgc ggaaaacttg cctgcaaatt
     5941 tagcagcatg ggcgcaatcg atcaacgatg ttcaagcctg gctcgtttcc tttttgattc
     6001 agcacacatc tgtgactgct tcaaaatcgt tttcagaaca gctactgaag ccccttcttt
     6061 actgacgttc cccatacaaa accttacaaa atcagaaatt cagaattttg tatattcaaa
     6121 aaagtttttt tgactgcata cacccactca cacgatcgaa taaaccacac aaacagtatt
     6181 atctagattt ttagatcgta catcatttaa caaacagaat attattctgt tttttagatg
     6241 ttatccagat cattagatcg ctcttgtggc attgacaggt tttaaatgtc gcacacacaa
     6301 tactatccag atgattgtaa aaagctcgtt catcgcgttt tttgtgctca ttgcctggaa
     6361 aatgagcact ctttgacgcc cgttagaacg cttgtcatct catgctcttt aacaaccccg
     6421 gacagcggtc attgcctaaa gccctggcat aaggaggtta ttattcgtgc atttcaagca
     6481 ccgctggcat ctgtcagcaa cgggctacgt aacagagtga cgtgccgcgc catctacaca
     6541 ggtaacgact ctttatccgc aaatatcatt ctgcatactt aaaccccctt atttggtgaa
     6601 ctcgcgttga gttaatctca ctcactctct ccctgagcaa gtcgtacagg ctgcaacgca
     6661 gaagtacacc agatttgcca gtctccactc caggtaaggg ggtttagcta tgcatgatga
     6721 tgaacaatca gataaagagc ctgtagtgag tggctgcaac ttttcataat tagcttatga
     6781 cgctgtattc cggggatgtt tctatttcag acaaacaacc gggagaaaat atggcgcgta
     6841 agttaaaaat taatcctgtc attcctgaat gtgtggcaga tttgaaagga tatccactct
     6901 atatcatcgt ggcttattgg ggattaagaa ataataaaac gctcacaaca acggatgtta
     6961 gccgtaattt ccttattaca cagcaacaat cttatgatgt gcttacctac atttaccagg
     7021 aagcacagaa gcagataaca gcaactaaac gaacactatt taacgagcac aaaagaagaa
     7081 ttagtgcgtt caggattctt gatattgaca aaggcatatt gcgcacccca aaaaaagaga
     7141 ttgcatatgc cagctacgac aatataccaa ctgtcacaat gaaaagtacc cgttgtgaga
     7201 tccaggatag ttttacagaa ctgcgccgct ggatgtgttc aagaaagaca ggtgatcgag
     7261 tggaggtatt tttgaatgtc taagtaccaa ataaacgcaa ccatcaaaaa accaggtggt
     7321 ggccccgtga aatggactca ttacagcgac ttccctatga ccgctgaaga atgcagaaaa
     7381 aacttctcaa aagaaaaaga agcaggaaag tgtttttctg ttgatgttca tatttcagat
     7441 ttcatttgca aagagatacc tccacaaaat gcaggaaata catatggctg attttggttc
     7501 cactaaacaa accgtaacat ttgaagaatg gcacgagcta ttaatggatt acgcagagtt
     7561 acgcggtggg aatgccgccg atgcggaagc atggcgtggt gattatgaag caggaaaaac
     7621 tccggtagaa gcatattgtg atgaatgggg agaggattaa tcatgattga cgctataatt
     7681 acgccaatag ttacatctat tccggtactc ttattaattt tagtcgcgct gatattcgtt
     7741 aaatctgccg tcaaaatcgt gccgcaaggt aatgcctgga cagtggaacg ctttggtaaa
     7801 tatacccata cgctttcccc cggtcttcac ttcctgatcc cgtttatgga ccgcataggt
     7861 caacggatca acatgatgga aaccgtcctg gatgttccta aacaggaagt tatttcgaaa
     7921 gacaatgcaa acgtaactat cgatgctgtt tgttttatcc aggtcattga tgcagcgaag
     7981 gcagcttatg aagtcgataa tcttgcatca gctatttcaa atcttgtcat gaccaatatc
     8041 cgaactgtcg ttggtggaat gaatctcgac gatatgcttt ctcaacgtga cagcatcaat
     8101 tctaaactgt taactgttgt cgattatgca actgatccgt ggggaattaa ggtcacacgt
     8161 attgaaattc gggatgttaa accaccagaa gaactgacta aggcgatgaa cgcgcaaatg
     8221 aaagcggaac gtactaagcg tgctcagatt ctggaagcgg aaggtatacg ccagtcacaa
     8281 attctgaagg cggaagggga aaaacagtct caaatactga aagcggaagg ggaacggcaa
     8341 tcagcgtttc ttcagtctga agcacgcgaa cgtcaggcag aagcggaagc tcgcgcaaca
     8401 aaactggtgt cggatgcaat cgctgaaggt gatgttcagt ctgttaatta ctttattgcg
     8461 cagaaataca ctgaagcact ccaggcaatt ggcacagcat caaacagtaa actggtgatg
     8521 atgccgcttg attcaagtag cctggtgagt tcggttgcag gtatcagtga actgctaaaa
     8581 aacgtatcac aggatacttc caaagttgat atgaaacagg ctcaacagta tttgtcttaa
     8641 acagataccg gacggtgaca atattgtttt aatcaccgtc cttatctcat ggagattaac
     8701 gatgttctgg tttacgctgt ttcttctttg tattgccctt gaaattataa ccggaacggg
     8761 atggttactc gtaatcagtc ttggtgcatt atcaagtgcc attgttgact ttttccttcc
     8821 tatatcacaa gaagcaaata tatgcttctt tgcgtcaata agcatactga cgtcgataat
     8881 taagttcgtc tatgacaaaa aacataagaa attagatacc cccctggtga acacagggca
     8941 ttcacgtttt aaaggaaaag aatttacgct cagtgacgat attgttaacg gcaaggggca
     9001 attacttatt ggtgatacat tctggccagt tgagacgtta cacaatgaaa actaccctgc
     9061 gggaacaaga gttattgtta cagacatgca aggaataaca cttaaagtca tgaccatcga
     9121 agacaagaat gaaaacaagg aataaatcat gcatctttcg ttgttaattc cgcttacagg
     9181 agcagcgatg aatgcactat gtatttttat tatcagactt ctggataagg attttattga
     9241 agcctacaaa aaaacgctaa aaaacattac tgcattacat attttattga tggtgttaag
     9301 tgtcacagga atagtatgag gaaaattcta tgcgaaacgc aaatgaagga atttctcttg
     9361 cagctattgt agctaaagaa acaacaacag gtaaatggaa tggtactggt gggggtattg
     9421 gtttaggtac tggtggtatt ggcttattca tcggaggtgt ttctggttct aaacaagagc
     9481 agacacaaag ggcaaaaaac tttgaatcgc ctaaagagga agaatacagt ctattaaatg
     9541 tatacggttc gataataaaa gttctcgttt ttggcgtgct atttggttta tctgcccgtt
     9601 tatttacatt tttatcagac actgtttctg attttgctga accagtaaca accaccacag
     9661 ccacaggaaa gattcaatct attctggatg gcatggcaga aatggtaagc attttaggca
     9721 ccgttgtccc ttttttagta attattttag gcaccgttat ccctttttta gtaattatca
     9781 tagcaatgat ctggtttctg tttttcagca gcaaacgaga aaaagaagag ttaactcgag
     9841 ttgaacgtga agctgctaaa acaaaactcc gtatagatgt ctatcaccgt ttacgttacg
     9901 ttgaaagcga tcatgtcgtt tttgacccga ttacaggccg ggaggttccg gcagaacggg
     9961 cgtgtattaa taaacttatt gaggcacttg ttgatgaaag caacgacatc tcaaaaagaa
    10021 attgacgaac ttctgaatgc aataaaaaca ggacattcaa aattcattct atcaataccc
    10081 gtacatacta cccatccaat ggcccataaa ataatgaagc cttacttcag gagaacagga
    10141 ccgcttctgt attcagatag tatattcagg atttgaagaa agtatttatt tatatagggc
    10201 atacaaaaat gagtgctttt attaagctgg catctgttct ggcgttattt attagcccgg
    10261 ttgtggttgc aatagcagga gaacaatctc actgtacaaa aatcaacgaa taccctttca
    10321 tcgtcactca atgcgacgac ggaactgtga cagtggtgaa tgtgataaat aaccgtgtat
    10381 ctgtgtgccg taaaggggaa tcatgcaaag aaataaaatt ataactctgg acacattaca
    10441 tgactcaagc aaaagctaat ctgagaaaac ttcgccgtgc acgttatgtc ccccctcgaa
    10501 cagtaaagaa aagcatagcg aatacagaac gtgtcgtttc aaaagcaatt accggctgtg
    10561 gtgtcttccg tgatatacca caagtaaaaa tcaatctcat cgaaacaagg aaaattttac
    10621 aaatcgaact ggctttatcc attgctaact tatctgaggc gaatccaatt gagatacgaa
    10681 aaaaaatcaa aagcattgac cgacaattat cttcaacctc aaaaatcaga actacattat
    10741 gaaaaaaaac gacgatttaa tcactttacc atctcataac agccacgctc tcgattacat
    10801 cattaaaaaa gcgtttacac cacgtaaaac caaaacatcg acagcaaccc acaacgttta
    10861 tagctacaat aaagatgaat ccacattaaa tttccaaaaa attggagaag caattttttt
    10921 cggtacagac ctggtaatta agctgaagcc aaatattagt gcctctggtg aactcttcat
    10981 ctgccagaag gactaagcca tgaacattgt ttcagaattt cttgagaaag taacaaccat
    11041 tgttgaatcc acagcggcga cgaacgccgc taaggtcgca caagcaatat ctcctgtatt
    11101 ctttgcggct atagccttat acgttattta tctaatatat gaaatcacgt atgcccaacg
    11161 tgacatcctt atgaacgagg tgacgaagaa tatcggtgca ttcgccctgg ttggtgcatt
    11221 cacttattca gcgccttatt acagccagtt tgttattcct tttgtcatgc acgctggctc
    11281 cgacctttct gctgctgtta ctggaggttc aggaactgca accagtgttg ataatttgtg
    11341 gaacgctcta tctacgaatt taaacaactt ccttaataca agtatgagta tttatggaaa
    11401 gttccaaatt gctgagaaaa tgggggttta tctagtatgg gggattggct atatcggcgg
    11461 aatgcttctc atctattaca ctactgtttt cttgacacta tcaactttca tggttggaat
    11521 tcttctttcc gctggcattt tattcatttg tttcgctcta ttttcatcga ccagaaatat
    11581 gtttactgca tgggtcggca gttgtctcaa ctatatactg ttaaatttat tctatagcat
    11641 atcatttagt ttcttaatta gtttcgttga atcagttgtc cctgttaatg gagatataaa
    11701 actatctatt gtagtttact ttttccttat tactattatt tccatattcc ttatagaaca
    11761 agttggcaca ctctgttcta cattaacagg tggggtaggt ataaatggcc ttacatcagc
    11821 cgctaatggc tttggaggta aggttgcatc tggttttatg cgagcatcag gactccgatc
    11881 tttcactaat ggatttgcca gcaaaatgcc aaatccaatc tttaatgcag gaagtcgttc
    11941 tgcatcagca ttagttcagg gattaaaccg tggaagtaaa cttaaagcgg ggtgattaac
    12001 atgaaagata aagtattatc atttctcttt gctggaataa ttaccgtaac atcaccatca
    12061 atctttgcat ctggtattcc agtagtagac gttacagcga tagctaaaac agttgaagaa
    12121 gggctaaatc gtgccgctga agcggcaaga cagcttgagc aactcaagca acaatatgaa
    12181 cagaccataa gatacgctga agaacagaag aaaagactcg aaggcttcac tgatttcagc
    12241 aatggctttg atagcgcatc cagctatatg aaggattctc tatcaacaat aacaaatagt
    12301 gcaaaatcag atcttagctc tttgcgatcg caatatgact tatcaagcaa cattgcagag
    12361 acgcaacaaa aatatgatgc aatcctggct aagatcaaat tctatgaaaa tttcaacaat
    12421 gaaatgcagg aaagggctaa gagattaacc acgttacaga aggagttcgc gtcagcagat
    12481 actccgcaaa aaaaagctga cttatctaac caattgaata cagaaaaatt gacaatggag
    12541 ctacaattga agcaatatga tattgcagag agacagctaa tcgcgtctga aaatgcaaga
    12601 caagaggctg ttagtcgaga aaagtttaaa ttaatgatgc aataggtgtt cgaatgaaaa
    12661 aattcataag cactttaact atcttgtcat gcttcctttt agctgcttgt gaagacaaag
    12721 tttatgatgt cagttactac gctgaacatc ttgaacaagc ccaagatgtt gtcgaaaaat
    12781 gcagcaaagg ggatatgtct ggtcaaaact gcgaaaatgc gcgtgaagcc attcaaaaag
    12841 agcaatcggg taaagcattt aaaaatatga tgcaataatc cttcattaaa aacagctcgc
    12901 aaagcgggct ttttttggct gtacgcagtg accagttgat aaatagttct aatgttcgtg
    12961 ctgcattaga acaacgcaca aaaagaagag tacaagaaga cagtgcggga tctaatggaa
    13021 cgtttacaat agaatcacct tccctatagc aaggtgattc tatttaaaag aatacccgcc
    13081 tcgtgcgggt tttatcttgc ttaaagatct atgaaacctc cggcttcggg tattaaacta
    13141 taacttgctt tggcaaatat tgtttcattg acttcatgga atcttatttt ctccgcctgt
    13201 tcatatgtta cagggaagaa cacgccccct ggctcattaa acatcataaa atcaccttgc
    13261 attgcgttac aatgacaaca atgattcgcg taatagttca tttttgctgt tttgctatgg
    13321 gatggataat ataattttga atactcatta attcttgaga gagcagaggt tgaaattatt
    13381 tcaattttcg ttaaatcagt ccaccatact ctactggtta accaccgata aatcatattt
    13441 ttctttgaat caaaatactc cataccctta ggggaggatg tttcatacca ttcatctaat
    13501 atacaatctt cccattcggc aggatcaact ccccaaataa cgtctctata cttataccaa
    13561 cgaggtaaaa aaataccaaa cacaggagtt gttttcttac agcgccaaca agactggtaa
    13621 ctttccgcaa tataaaaacg ttgcgaaaaa acattgtaat catgttcatt taattctttt
    13681 atccaccgaa taaaataaat tggatttttt ccctgtggaa caaaccattt ccgttgctct
    13741 tggtcccatt gcgccccttt ttgtttagcc aaatcctttt cttcaaacgg aacatttaaa
    13801 taaaaccgcg ccatagccaa cttaactcca gtattctgtt ctgatgatgc acctggatag
    13861 atatcaaaac taaacaaaga gtgcaattcc gcctctaatg gctcattcat acccgcctcg
    13921 tgcgggtttt cttttgcctg aaatatggag gaactccatg aaatccatca ttttatcgat
    13981 ggttgccgcc accgcattac tgtcaggctg cgcggatagc aaaacacaac cagtacctga
    14041 gtcaccgtta acctcaaatg accaccagat aacgttgaaa attgataccg tgcgaacaga
    14101 cctgccggac agttctcctg tctgtttgaa tggtatgaat atatacgaaa gtctgagcga
    14161 gcggcatcca ggcttgtttg ccaaaaatca gaacgtgatt ttccgtagtg cattctttga
    14221 tgacggtcag gcgatatcct ccgaaaaaat ccgcaaaata atgcagagta ccccgtgcgt
    14281 tgaatcacaa acaacatctg ccggaagctg ggaacctgcc acggaactga tcccccgtca
    14341 ggcaatatcc ggtgacacta cactggctga aaaagatcgg actgctggct tcacaatgcg
    14401 gatagcaccg gagattgagc gtggaaattt ccacctgcta ctcacaacca ctgacccgat
    14461 acggaaacag actgtcgagc agaatatttt actcaaaccg gaccagcccc ttatcgtgag
    14521 tggttttact ggagttaatg ctgatagcca aagtgagaac cggattgtca tcattacgcc
    14581 acacgtgcgt tgattcccct gatatttaaa ttacccgcct cgtgcgggtt ttcttttgtc
    14641 tgaaatatgg aggaacacca tgaaatccat cattttatcg atggttgccg caagtgctat
    14701 cgcactctct gcccccgtat atgcgtctga tccgtgtgct tccgttcttt gtctgtacgg
    14761 taaagctatc ggtagcggcg gcgggagcga atgcaaaagc gccgaaaagg acttctttaa
    14821 tatcatcaaa aagaaaaagg gatctattcg ctggagcaaa acctttgatg cccgaaaagc
    14881 gtttcttaat caatgcacga ccgcagaccc tgcggcgatc tcaaaaatca tgagtaagtt
    14941 cggacgttcc tggggttaac catgaaaaag ctcattatta ttcccaccac attattgctg
    15001 aatgcgtgtt cccatgcccc tgaacccgca caggcaaaag gaggttggta tgaactgaat
    15061 accacaattg agcaggtaag agcagacaca tactataatg gggtgatcct tcagcagccc
    15121 gtgtcacagc caaacagcac tgttcaatac agcaatgata attatctcaa aaaggaagcc
    15181 ataaacgtaa ctcccgcgcc tcgtattgca gaaaccgtaa aaccacgttc tgagcaatca
    15241 gcgacagcgc caaaaaacca gggcagcaat aatagttttc tgaagccgac aacaaacagc
    15301 ataacttcat ctggtgagat aaaaacagca gccaaaggag caactaaaca gcattctgaa
    15361 caaccagcac tccctcccgc aaagccaatc agtagcaaca accatcaggc acaaacggca
    15421 caaaagactg atcagcagac aaaaccgcgt cctgaacaac caccgctaat gtcaacaagc
    15481 ggttttctga aaaaaaacgc taacaacgct gttccggcca ccacgcttac aggcccatca
    15541 acacctgaaa agcaaattta ctttgttcgc catgatggta aaggctcgct gcaaaaagca
    15601 gtaaaagaaa ttgtcccatc ggactggacg gctgaaatat ccccggaggt aacgaaaact
    15661 ttccgtcgca cgatatcctg gcagggcaac gatcaatggc cttatgtcct ggataaaatg
    15721 cttcgcaact acggtctgac ggtaattaag aacaccgaaa aaagacatct tctgattgat
    15781 tttcaacaac agaaagggaa aacatcacaa aaagcacctg tatctcctga caaaacccct
    15841 gcatctcctg tcaggacacc agtttcctct gacaagttac ctcaaaaacc gctaacgcct
    15901 gtcaccgttc cgcaaaaaca gacagaaaaa ctcacctgga atcttaaaaa aggttcgacg
    15961 ctgcgggatg gcctcaccga atgggcgtca agttcgccgt gtggtacttc aacctggaat
    16021 cttgtatggg atactcctgt gaactacaga atagatgctc ctcttatttt ccggggaaac
    16081 tttgaatctg cattaaaaga tacgctgatg ctttatcttt cagcagataa accgttatac
    16141 gcattcagga atattgcaca atgcgtaata acaattaaag acacacaaca ggagtaatcg
    16201 ctatgggttg gttcttcagc ttgctcctga tattcctgtc aataggcagc tactatctga
    16261 cagataacaa taaccaaacc atacagcgaa tgaagttgct tcctgcaaaa caggaagcaa
    16321 ttgaatttat acatttcgca aatgttatca atgactatct ttataaatat ccagacaaac
    16381 gtaattcagg aggaacgtta acatctgagc aaataggcat aacccccgtt tatgatattc
    16441 atcatattat ttacggtaag cgagtttata tctggtctgc cgatacagaa ggtctgatgt
    16501 ctgctttgca gcagcaaaca aaacattcag caatgttagg aagagtaaaa aataagaaga
    16561 ttgtcgataa tcaggggaat gatatgggcg ttcccgttcc ttcctccatt ccagaagagg
    16621 ctattgtact cattaactaa atatcccctg acaaggggat tctgaactgg agtctgcata
    16681 tccgacacat cattatgtgg gccggatata tcgctacggc gcggacttca gtccagaaaa
    16741 tctttaccgg agatacccat gcaggataat gatgatgtca gcgagcttga tattgccact
    16801 cgtgaagtcc ttatcttacg atgcaatctt attcagcttg cttctgaata tataaaaata
    16861 aatcctctta aaaacacccg aatctacaca gatttaattc atcgttcagc agatgttatc
    16921 aaaaatatcg aacaaggtaa catccagaat aaacataatt atctgtcaca ggatgtaaaa
    16981 catacgcttg ttttatacga cctgtacaaa tcagactttg aacaatgtcc gggggcaaaa
    17041 tcccttatac ctgtcaggga aaaaattgtc tcgctgaaag aacttctttc cggcgctaat
    17101 gagctggaca gtatcgttcg tcgcccccgc aggaaaaaac gttcttcacg aatttaatat
    17161 caggagataa atcccttatg cagttatcag cggctgttct ggcagcactg ataagtcagt
    17221 gcgcccctga tgtatcaccc gatacgatga atgccctgat tatgactgaa tcaggggcta
    17281 atccatatgt aatagcgaat gtttctgacg gaacaagtaa gtattttaaa gatgaaaaag
    17341 gagctattga atatgcagaa aaattaacag cagaaaataa aaggtttagt gcaggtctga
    17401 cacagatata cagcaaaaat ttcccatcac tcaatctgac taataaaaca gtttttgacc
    17461 cgtgtacaaa tataaaagca ggagcggcag tcctgacgga caattatctg agacaaaagg
    17521 agggtagtag taatcaaaaa attctccgcg ctttatcttt gtattattcg ggtaacgaat
    17581 caacaggatt tattaaagaa aagaaattta acaacacttc ttatctggag cgagttattc
    17641 gtaacgcgaa taactatatc gtgccatcaa tccgtgaaaa aactgatgaa tcagaaataa
    17701 cccccccgaa cgatgaagag aaaacatcac cggactggga tgtttttgga gattttagtt
    17761 aatgaaagtc aaatcaactt tgcagtattt tatcctggcg tttttcctgt cactaactgc
    17821 ctccgttgcc tatgcaggtg gactcgacac cgcaacaaat accatgacag aacttaagac
    17881 atgggcattt agctttggtg gtgtttgtgc tttgtgttac ctgatctata acgtcatcat
    17941 ggcattcatg gagaagaaaa gctggtctga tgtaggaatg gcgcttgtct attgttccct
    18001 cgctggcggt gcactttacg ccgggaactg ggcgctggaa ctcttcaaat aagtaattca
    18061 tatatacaac tgtgccgcat atttacctta cgtgtgagtt gcggcttttt tcatggtgag
    18121 ttatggacaa agtaaatgaa catgtcagtg agtcattcat gacttataac gggtttaacc
    18181 gtcctgcttt agttgctggt atacccttaa tgttgttatt gttcacggct tttttcgctg
    18241 ttctgacagg cttccccgct attttccttt ggggtataaa agggataatt attcctgtca
    18301 tatgtgcgct gtttcttttt atagtaaaac tggcctgtga aaatgactca aacgcactaa
    18361 gagtcatccg tttaaacctt atggggcttt tgttaaaaat ccgccacaga gatttaatta
    18421 ttggttattc atctgtcagg taacatatat gcacaaagtc aaattaaaaa acagtgacaa
    18481 tatggatgtt gctcgtgaat atcccgatta tcgcttccat gtaacagaca gtattatctt
    18541 tacttctgac aggaaaatgc tggcttcact tgttgtagca ggcattccat ttgagacaga
    18601 gaacgataat gttcttacaa atttatttaa cagcgttaaa aacttcctga ttgggttagg
    18661 aaaggaaggc gacctgtatt tatggactca tcttatcaag aaaagagcaa ccatcaatgg
    18721 agaatggaat tttgacaatg attttttaag caggttttca aaaaaatacc ttgctctgtt
    18781 cacatccagc gcattctata aatcaacatg gtatctgaca tttgggattc cctacgatga
    18841 tgttgatacc ggaatcgagc gcatgaatga aatgacacaa caggcgcaaa aagcacttct
    18901 accatttaac gcttctttat tatccgttta caatacttat attagtgagg tggcagacta
    18961 tctatcacta ttgctaaatg cagaacataa catgattcct ttatcatcca cccccttatc
    19021 atccagtatt tgtgattctg aatggtattt tggtgctgat gttcttgaac tgcgtaacaa
    19081 cgaatcggat agtaaaaaat tcgcaacgaa ttatattttg aaggatttcc cgatagaaac
    19141 aacccccgga caatgggatt tccttctcaa gcaaccttat gaatttattc ttactcaatc
    19201 gtttattttt gaatcaccca caaaaaccct gaagaacatt gattcgcaat taaacaaact
    19261 gcaatcagct aatgatgcag ccaaaacaca acaggaagaa ctcgaagctg gtaaagaagc
    19321 ggtcgcagcc ggaattacgt tgtttggttc actacactgc gcattaacgg tattcggtga
    19381 tacaccagat caggccagaa gcaatggtat aaagctctct gctgagttta ttacttcggg
    19441 taagggattc cgcttcagtc gcgcctcact cgcatcacct tttgtttttt tcagtcatat
    19501 gccgctgaat aagaggcgtc cactggatac ccgaagaaca attaccaacc tggcctgtct
    19561 gatgtctttt cacaattatt cctccgggaa aaaatcaggc aatcctattg gtgatggctc
    19621 cgccataatg cctttaaaaa cggattcagg cagcgtttac tggttcaata cacattacag
    19681 cccaccggaa aaaaatgtta ccggacaaaa aattgccgga cacgcaatgt ttcttggcgc
    19741 aacaggcaca gggaaaacaa catttgaagc aacagccagc ggctttcttc aacgctttga
    19801 ccctttaatg tttgttgtgg attacaaccg ttctaccgaa ctttttgtcc gcgcatacgg
    19861 agggagctat ttcacattgc aggaaggtat ctataccggc tgcaatccgt ggcaactggc
    19921 agaaggcccg gaatcgcccg tctggcacag acttctcgca tttctgaaac gctggacaca
    19981 ggttctggca cgagacaacc agggcaaccc ctgttctgat gaacatggca ttgaattaaa
    20041 cgctgccgtg gaaagcatta tgcgaatgcc tgtcgaagaa cgccggacag cccttcttct
    20101 cgatatagtc agtcctgaac ttcagacgcg tctcgccaaa tggtgcgaca acggcgaata
    20161 tgcctgggcg gtggattccc cccggaatac cttcaatcca ctgcatcata aaaaggtggg
    20221 atttgataca accgttgtcc tggacacaaa aaacggtatt catcctgcct gtgaaccttt
    20281 actcgcggtc ctgtttttct tcaaagaaat catgcaacgc ggcggcaatc tgatgctctc
    20341 cattattgaa gagttctgga tgccagcaaa ctttcctatg acgcaagaca tgattaaaag
    20401 cgcactgaaa gcggggcgca tgaaaggtga aatgatgtgg ttaacatcac agtccccgga
    20461 agacgctata aactgcgcaa tttttgcggc gctggtacaa caaaccgcga cgaaaattct
    20521 cctgcccaat cccgatgcaa aatgggaagg atataaagaa attgggctta ctgaaaaaga
    20581 gtttgaaaaa ctcaaagagc tgacgaagga gtcaaggacc atgctcatca agcaatctgg
    20641 ctcttctgtt ttcgccaaaa tggatttgtt tggattcgat gagtttatcc ccgtgctttc
    20701 tggctctgaa actggcctgt ctatttttga tgagattatt gctgaaaaag gagatgtagc
    20761 tcctgatatc tggataccag aactcctgaa acgcctgaac ggttaatttc tctgttttat
    20821 acagaacgga aaacaatatg ccccccctca taattgcctc cgcgctatta attagtgcat
    20881 gttctcacac cctcaatcct gtgcagaaaa aagggggatg gtttgagtta aacacagcaa
    20941 ttggacaaat caaagcaggt gattattaat ggaaaacaca aactattatg aacattcaaa
    21001 aaaagaagcc tccaaaaaga aatttgaaga aaagaacgaa aaaaaggatt atttcaaggc
    21061 catacgtgac tttgagcgca gtgaaattga aatcataaag aaaaaggcaa aaacatttac
    21121 cattctcgcc attggtgaat ttgttgtaat ttgcattctt ggtttcgcaa tagcctccct
    21181 tgctccatta aaaaccgctg taccgttcct tgtgcgagtg gataacagca ctggatatac
    21241 tgatattgcc cctcaattat ctgatgctaa agaaagttat caggatgttg agactaaata
    21301 tttcctgtca aaatatctca ttaactatga agcatacgac tggcagacta tccaggaaca
    21361 ggccgataca gtaaaaacaa tgtcttccca aaaggtgttt tcagcttatg acaccatgat
    21421 tcgtgctgat tcctcaccac tgaacattct gaaaaacaac tacaaaatta aggtccagat
    21481 taattcagta atccttctaa ggaaggatat ggcgcaggtg cgtttcaaaa aaatggtact
    21541 ggatttatct ggtaaacccg cccccggata cagagcaaca gagtggattt cgacgatttc
    21601 ttttgactgg gataaagata tcaaaacaga aaaagagcgc cttgtgaatc cgctgggact
    21661 gcaagttctg agctaccaac ctgatccaga ggttataaaa tgatgcttaa acgcactact
    21721 gcgatagttc ttttgtcgat gctacaagta cctggtgctt tagcagctat gtacggcaca
    21781 ccatcggaac gagacgggcg tattcagacc gtagattata acgagcagga tgtatttaat
    21841 gtgcgcgtaa aagcaggcgc acaaacaaca attaagttcg gacaagacga aacaataaaa
    21901 gatgtcggaa ttggtgatcc agaagcctgg tcagtatcgg tcagggacaa cactctgttt
    21961 ctgcgtccaa aagcagaaga accggataca aacgttaccg ttcagacaaa taaacatatt
    22021 tatcctctct acctgatatc gaccacaaaa caacccacgt atattctgcg ttttaattat
    22081 ccgaaaccac cgtcagctac cgtaatgact gaaaggccat ttccatgcac tgatggtgga
    22141 ataattaatg gtcattatca actcaagggg gacaaatcaa ttttccctta tcaggtatgg
    22201 gataatggcg aatttacatg tatgagatgg actaataaac aggaaattcc ggtcctttat
    22261 cgtgttgatg ccgatggaaa tgaacatctg gttaacggag acagaaataa aaacacaatg
    22321 gtttactatg acgtggctga aaatctccgt ttgcgcctgg gtgatcaggt tgcagatatc
    22381 agaacatcat caattgttaa tcgtccgtgg aataaaaaag gtacgtcaaa cggaaaaaca
    22441 cgagtagaga agttcagtta tgaaaaataa agatgatgaa aaaaataatg acgcgggaaa
    22501 tcgcggaatt atcgaagtaa aaggtaaagc agcgcccaaa aaaatattaa tcctcatcat
    22561 attactgatt gccgcgttat ttgtgatcat tatcttgttt aaagttcttt cacgagaaca
    22621 ggtcgttcag caaacacctc ttgagaagtc tgacgagaca ctggtcacaa ataccaacaa
    22681 tggcgtttca ctgacaacaa tgatgaaaaa catcgaagaa aaggaaaaac tcgatgccgc
    22741 taaccgcagg aaagcacagg aggagcaaga acagaaacag gcagacaatg ccccgtcagc
    22801 gccagcatca caaaaggccg atcaaacagc cgtcaatgtg atagccaacg gcactcccca
    22861 aaccgccagt ggagatccaa accaaccaca accattgcca aaatccgtcc gacaactgat
    22921 gggcgacaca atggtgaaga tcgacaatca ggaaccaggc gaaaaaaacc aggaaagaga
    22981 tgatttacag ggcagtcagt acgcagacgg aaaggtatcc cctgtgctga accgccgata
    23041 tctgttatct gcgggaacag cattatcctg tgtgctcaaa acgaaaatcg taacctctta
    23101 tcccggtatc acaatgtgtc agttaacacg agacgtctgg tcggataacg gcgaagtgct
    23161 tcttgcccgt aaaggagcat tactgatcgg ggagcagaat aaagtcatga cacagggagt
    23221 agcccgtgtg ttcgttaact ggacaacgct caaagatgaa aatgtcaatg tgcgcattgg
    23281 cgcacttggc actgacagtc tgggcgcttc cggccttcct gcatgggtcg ataatcattt
    23341 tggtcagcgt tttggtggtg cattgctgct gtcattactt ggtgatggtt tagatattct
    23401 gaaaaacagc acgcaacaaa caggctcaaa cagcaatatc acctacgaaa acacatctga
    23461 cgcaacaaaa gaaatggcaa aaacaacgct ggacaatacg ataaatattc cccccacggc
    23521 atatattaat caggggactg tattgtctgt tattgttccg cgaaatatcg acttttcatc
    23581 cgtatatgaa cttcagtaat tcagtaaagc aattgatatg aataacatca gagataccag
    23641 taatacatca caaccacgag ataacagtaa aaccgcaaga cgatatctgg atatgacggg
    23701 tatccagtct gttcttaata ttgaacatgt cacagaaata tctgtgaata aaccaggaac
    23761 aatatggttt gaagggaaaa atggctggga atctaaagat gcgccagacg cgacttttga
    23821 taatttaatg acacttgcca aaactctgac gaacttatca aagataaaaa taccacttag
    23881 ccatgataat cccattgcat cggtagttct tcccggtggg gaacgtggac aaatcatcat
    23941 tccacctgca acagaaaata acagcgttgt tatatctatc cgtaagccat cattaacgcg
    24001 ctttactata gatgattatg tccgaacagg gcgttttgat aatgtcagga ttgcgacgaa
    24061 acacgaagcc atcctgactg aacggcaacg ctacctgtat gaattatccc ggcgtccaga
    24121 tggtcaatct aaagctcaat ttttaagaga ggcagttaaa gaccgtctta attttttaat
    24181 cgtaggtggg actggctccg gtaagacaac tatagcaaag gcaattgcag atatctttcc
    24241 acctgaacgg cgttatataa ccgttgaaga tgtaccggaa atgtctctgc cgctgcatcc
    24301 aaaccacatc cggcttttct acaaaaaaaa taccgttgaa gcgaaggaaa tcattgaagc
    24361 gtgtatgcga ttaaagccag atcacatctt tcttgctgaa cttaggggta acgaagcatg
    24421 gagttatctg gaggcgctta atacagggca tgaaggctca atcagtacca ttcatgctaa
    24481 taatacttat gcctcatttt cacgactggc aagtattgta aaacaatctg atgttggtat
    24541 gactgttgat atggacctca tcatgcgcac gataaaaaca tcaattgatg tcattctttt
    24601 tttcaaccat accagaatga ctgaactgta ttacgaaccc gaagaaaaaa atcgcttttt
    24661 gagcacaatg taaattctgg agataactat ggatgcaaaa aaaacggggg ggttaattct
    24721 cttcctgtta ctgcttcttg tgggtgtgct tatagcctca aactatctcg gtggatacac
    24781 agcattaaga tattcatccg ttgatatgtc tttgctcaag tgggatacat tccattctgt
    24841 aatctcaaca ttttctggca acccacaata taaaaaactc gtttttatgg catggtttgg
    24901 attcagcgtt ccattaatat tctttgccat attcatgttg attgtcgtga ttgggattat
    24961 gcctaaaaaa gtcatctacg gtgacgcccg gctcgcaact gatatggacc tgagtaaatc
    25021 aggttttttc ccggataaaa aatcacctta caaacatcca ccaatactca tcggtaaaat
    25081 gttcaagggg agatacaaaa aacaatttat ttatttcgcc ggacaacaat ttttaattct
    25141 ttatgccccg acacgttctg gtaaaggggt ggggattgtt atccccaact gcgtaaacta
    25201 tccaggaagt atggtcatac tggatatcaa acttgaaaac tggtttttat cagccggatt
    25261 caggcaaaaa gaattagggc aagaatgctt tctctttgcg cccgccggat acgcggaaac
    25321 tatcgaccag gccattaaag gacagatacg ctctcaccgc tggaatcctc tggattgcgt
    25381 cagtcgcagt gatttacttc gtgaaacgga cctggcaaaa attgccgcaa tccttatccc
    25441 ggcaagtgat gatcctatat ggtctgattc tgcacgaaac ctttttgtcg ggttaggact
    25501 ttacctgctg gacaaggagc gttttcacct ggaacagaaa gccaaaggtc ataatgtacc
    25561 ggacgttctt gtttccatca gcgccatact gaaaacatcg gttcccgatg gcggcaagga
    25621 tctcgccgca tggatgggac aggaaattga aaaccgtagc tggattagcg ataaaacaaa
    25681 atcattcttc tttaagttca tgtcagcacc agatcgcaca aggggaagta tagaaacaaa
    25741 cttttcatca ccgctgagca tcttcagcaa ccctattact gctgaggcga cgaatttcag
    25801 tgattttgat atccgggata ttcgtaaaaa gcccatgtca atttatcttg gtctgacacc
    25861 ggatgcttta atcactcatg agaaaattgt aaaccttttc ttctcattac tggttaatga
    25921 aaactgtcgt gaactgccgg aacacaaccc ggacctgaaa taccagtgtc ttattctcct
    25981 ggatgaattt acatcaatgg gtaaatcgga ggtcattgaa cgcgctgttg gttttacagc
    26041 aggttataac cttcgcttta tgttcattct tcagaatgaa ggacaagggc aaaaaagcga
    26101 tatgtacggt caggaaggat ggaccacatt cactgaaaac agtgctgttg tgctttacta
    26161 tccgccgaaa tcaaaaaatg ctctggctaa aaagatttca gaagagattg gggtgcgtga
    26221 tatgaaaata tccaaacgct caatatccag tggtggtggt aagggaggaa gttcacgtac
    26281 acggaatgat gatgtaatag agcgcccggt tcttcttcct gaagaaattg tgtctctccg
    26341 ggataagaag aataaagcca gaaatattgc aatccgtgaa attataacca gtgaatttag
    26401 ccgaccattt attgccaaca aaataatctg gtttgaagaa ccagagttta aacgccgcgt
    26461 ggatatcgcc cgtaacaatc atgttgacat tccaaacctg tttacacaag aagtcatgga
    26521 tgaaatagca aaaattgcag aaatttatct ccctaaagct ggaggaaaaa aagtcatggt
    26581 tgccggaggc aatgtgatta ctaatccaga ccttgataat cacgataaaa cagacgtatc
    26641 tgaataatcc tcgcaataaa gatactattg attagcaaag acaggattct attatgagaa
    26701 taaaacactc gaatatgata aatatgaata tgaatatgaa tatgaatatg atgaaagaag
    26761 ccattgatgt tttgataaac tctgaaaaac atattctgat tataggcgaa actggaactg
    26821 gaaaatcaac acttttagca gaattgctaa tcaatgacac tgacgttaaa tattttgatt
    26881 tctgtgatat ttataaacgc catgaacacc ttccccttct aaaacaccat tatctttgcg
    26941 atgaaaattt tgattatttt gactttttat ctgcacctga aaaaacgttg gttcttaata
    27001 gtgtagccat cggtaacaac ttacgggaat caaaagttgt tcagtttatt gttcgattta
    27061 ttaaaacagc caggaaacgt ggcaaacgtc ttattgttgt cacgactcct tcagatggcg
    27121 gcgtgcagat acaaaatctt ttcgacacag ttatatcgtt aacccgctct cacgatgaaa
    27181 taggatgcac agttatcatt ccagtaccgg agttaattaa atatgaataa aaaattgctt
    27241 tgtttattaa tatcctcttt tttattatca ggctgtgcaa acttcaaaaa aatcaatgct
    27301 aatgaacttg cagccgaaag agacgcacaa aaagcgactg aatacgcgaa aaaaattcgt
    27361 aataacccgg ttatccagga ctcaacaaaa caatggatta atacaacgcc aattattgaa
    27421 aagcaggtaa agcaatcagc gccgccctgc ccgattgtga ttaatactcg ttcagatatt
    27481 gagctacgcc agatcgcaca acgcatcaca caaacctgtc atatcccggt acgaatcagc
    27541 gccgatgtct ggacctatct gaacggtaca ggcactggct caacacaaca aatgacagga
    27601 tcaattccag caccggatgc aaatggaatg gttcctctgg cgtcagtagg cagtcagcca
    27661 gtaaaagtct cttctggcgg tacatcactc ccggaactga atatctccgg gcttcctgcc
    27721 ctactgcaaa ccgtatcttc acggcttgga atatcctgga aatacgataa aggtcgtatc
    27781 acattcttct atctggaaac gcgctcattc ccgatcacat atatggacag caatgtggca
    27841 tacaactcaa aagttgtgag cggcacaatg tcttccagtg gcagtaccgg ctctaccagt
    27901 tctggcggta tgactggcga cgcatcaaat acgcaaacta caaccgtgga aatgaaatca
    27961 tcgctgtaca acgacctgaa aagcgaagtt tcctcaatgc tgacaccagg tacagggcgc
    28021 atgtatctgt ccacaggttc actgacagta acagataccc cggatgtact ggacagcgtt
    28081 caggagattg tgaacaagcg caataatgaa atgagtcgtc aggttgtgct gaacgttgaa
    28141 atcctgagta tcaagaaatc aagccaggaa caggcgggaa tcgactggaa cgcagtattt
    28201 aatgatggtc atcttggtct gagtcttggt ggctcatttg gtaatgccgc cgagaatgtt
    28261 attaccagtg gtgtatccat cgtggacggt aagctggctg gctcaaaagc atttctgaaa
    28321 gccctgtcgt cacaaggcag cgtcagtgtc gtaacgcaaa actccgctgt gactaaaaac
    28381 ctgacgcccg tccctatgca aattgccaat cagcagggct tcattgaaag tgtcacaacc
    28441 gattccaccg ccaatgtggg atcatccacg tcactgaatg cggcaacaat aacgaccgga
    28501 ttcaatatga ctctgcttcc atacattcag ccggattcac aaaacctgca attgctgttc
    28561 tcaatgagtc tgtctgacaa acctactttt gaggtgttcg aaagtggcgg cagtaaagca
    28621 caaacaccga atgtggattt gaaaacaatt aatcagacta ttgacctgaa atcaggccag
    28681 acagttattc tgagtggatt ccagcaatca aaccggaaaa ccagtaagca aggtgtaata
    28741 accccctcct tctttggact tgggggtggc attaacagcg aagacggcga cacgattctg
    28801 gtcgttttga ttacaccaaa tgtgcttaat tccggctcca gtgcagtaat aaaaaatgtg
    28861 aactaattaa ctgaaaactc cctgaaaaaa cacagggagt ttttttggag gtcactttgt
    28921 ctgagaataa tcagaataac cggaatttca cttcggttat taaaaacaag cgtgcttttt
    28981 tctccggtct ggactggaaa acgcttcctt cagaagaaaa gaatgcgcgt acattcgcaa
    29041 gaaaaaacga cgctgaatat tttctctcct gtcagtatca ggattcagaa aatgaaacca
    29101 aaacgatggt ggcatttatc agaaaagagg atcttcccac aggggcgtcc tcattctggt
    29161 cactggcact gatgataaaa cccctcatcg aacctgacgg atacgctatc tgcgaactcg
    29221 gtgatcttta tggctttgtt tcctgcgtca ataacgttct cgtcaacgat gttgtaggta
    29281 acaaaagtca gatcatgagt gctctgacaa catttcttga gtttaatgaa acaccagaac
    29341 ccggctggaa actataccag cctgaatcat gggatatcag ccaggcactc ccctcactca
    29401 ctctgtctgc gttgattgac gtaaaaaaac cgccgaaaga ggcggcgttt actcgcgtat
    29461 cccgaaaacg tcagttcatg atttatggcg gaagtgccat tctggccata ctgctctgga
    29521 acgggatcac catgtaccag gaataccggg aaaaagaagc tgcggcagaa gctgcacgtc
    29581 ttcgtctggc aaaagaaatg gcagacaaac aggcaataca gattgcccca ccgtggcaac
    29641 atttacctga aataaaacca tttattgata aatgcattga taaatgggat gcactgccct
    29701 tatcgatcgc cggatggcgt tttgaccttg ctgaatgcag cacatccggt aatgacgggc
    29761 tgttacgcac atcatataag gaactgagcg gcgttacggt cgaggacttc agcacacgga
    29821 tacgagaaat attccagggg acaaccacag caacttttgt attgccagaa ggcagcgccg
    29881 ggggattttc tcttcctgtc agttttgacg tttcacctga tccaattacc ccggacacat
    29941 tgccacaggc aacagatatt caggaacgcc tgacaacatt tgcacaaaaa atgaggttaa
    30001 aactcacctg gcaggaaatt gaaaacacaa aaacagatga agaaggccgt ccaataattc
    30061 tcccgtggaa cgagtatgaa ttaatgattc agaccagcac cccgccttca atacttttcg
    30121 ctaactttca tgaacctgcc gttcgcttcc agtatgcagg aataaaactg gaagaaggaa
    30181 gactgaatta tgagattaaa ggtgcattct atgttaaaaa taattaaacc aacgattatt
    30241 tctgtctgtt ttttcagttc acttgcttac gcaacagaat ctgaaagcac gccaaaagac
    30301 gaaataaaat attcatctcc gaccatttta caggtagagc agattcaggc tgaaacggta
    30361 atttacgaag caaagctggc gcgtcagaag gctgtcagtg aattgcgtaa tcttgatttg
    30421 aatggtgtag cgtccgcaac gccagcaata acgccctctt ccgggtattc ttctcttgca
    30481 gataccgata taccagcgca acgtgtagcc gctaaatcac ttcgtgttat tgaaatattt
    30541 ggtacaccac aacaaatgtc agcacgaatt tctctcctgg acgggtcagt aactgatgtc
    30601 aaggtcgggc aaagtattcc tggtacttct tataaggtgg catcaatcac cggtaatggc
    30661 gtctggattc agaatggtac agagaaacgc cagcttattc tttaatgcta ttgaaagaga
    30721 cgacacataa tgtttttttc tgacgcagac tatcccggaa aagatatttc tgatgttgta
    30781 tacgtcagta tatctgaaaa agacgataaa cctgttcatg tttatatcgc tgataacagt
    30841 aaaggacagc gtgctgttca ggcttatatt ggcactctga atgttaaata tcccggaaag
    30901 gtaaatataa cctggaccac tcttgatgtt atcgcatccc gctaccagga cagcgaaaga
    30961 aggtctgatt caaactcatc acaacactta aacagcaacc aggaaaaggt cattagctac
    31021 ctggcgaaag caaacaacct gggaagttcc gatttgcaca tcacacctgg tcgggacggt
    31081 tccgagttca cttatgtcga agcacgcgtg catggggaac tggaaattct ggatgtcatc
    31141 ccgcgtaaag aagggctgga attgctgggt gctgcctatt ctggaatgag cgatgttatc
    31201 aaaggtacgc agtttgaccc tgcaatacca caggatgcac gtatcgcaga gaattttctg
    31261 aagcctgtaa atctttttgg ggcgcgctat tcgcattatc cgtgcgtggg tggtgtgtac
    31321 gcggtcttcc gcctgattaa agatgacagc gaggatattc ccactttcga agaacttggc
    31381 tacatgccac aacaaatcca gacaatcagg cgaatgttac aacgcccgga aggaattatc
    31441 gttttatccg gccctacagg gtccggtaag tcaacgactc tgagaacggc ttcagaagcc
    31501 tatctgagca cgtttggttt caaccataac gataatatgc gtctcccccg aaaacgcctt
    31561 ttcaccattg aatcaccgcc ggagggccgc attcccggcg caatacagac ggcagtcagg
    31621 gattccgtcg atggctgggt tgacgcgatt aaatccgcta tgcgacttga tccagacgcg
    31681 atactgaacg gcgaaattcg tgatcacgca tcggcattag cagcaatcaa agcctcgatg
    31741 acaggccacc ttctgttgac gaccttacac gccaacgatg ccctgaatat tattgagcgc
    31801 cttgaaatgg agaatattca ggccaggcta attgcagatc ctcttctgtt tatcggtctg
    31861 ctgagtcaac ggctggtaca aaaactctgt ccttactgta agcgcactta tgctgaaatg
    31921 gcagacaaac tgagtacaga agaacggaaa ctcattgagg aaaattgcct gccggaacag
    31981 gtccggttgc gcaatcttga cggttgcgag cactgttata aaggcgtaac gggaaggacg
    32041 gttatagcag aagtcgtctc acccgacgca cgtttttttc agttatatcg tcagtccggg
    32101 cgtctggaag caaaatcata ctggcactat gaacttggag gaattacccg taatcagcac
    32161 ttattacacc tgattaatca tggcctggta gatccgctgt ccgcacactt catatccccc
    32221 attgacgaag ataaatactc aatgctcccg gaggggacat ggcgataaaa gatatatttg
    32281 agaaactcaa ttcgatagaa attgatttct ctggaatatc ccactggctg gcaaaataca
    32341 cattcaggta ttccagaata agtttatatg aagatttatc tggaatgtta aatgatggca
    32401 taaatgtaaa gcgtgctctt acacacctga ttgatgtaga aacagactac gggaagaaaa
    32461 cggggtcttc tgcaacctac tatatttgtt gtgagtgcct taatgcactg aataacacag
    32521 gggcaatatc ttcagcatta aaaaaatata taaaggagga tgaatatcaa ttaatatcca
    32581 gcggtgaaat tcgcggcgac cttgcgggag ggttagttca ggccataaaa attgtcagaa
    32641 gtcgttctga aatgacaatg actcttgtta tgtcgatgat ttatccatcc gtactggttt
    32701 ttggttgtct ggcaaacatg tatatggtcc acgataccat aacacctgtt ttcctttctc
    32761 tcgcaccgaa agaacgctgg acaagttcta tgcgattact gacagatacg tctggtttcc
    32821 tgatagaaaa cggaacgtat attctctgct tctgcatcgt actgtttttc ctgatcagat
    32881 attcgcttgc gcgttataca ggaccgggcc gtcagtatct tgattacatt cccccctggt
    32941 cactttataa aacatttaat ggtgtaagtt ttctcttcaa catggcctcc atgttaagta
    33001 ttgatattcc cgttgcaaaa gcactggaaa ggctggagaa aaacagcact caaaacagat
    33061 ggctactcga acgaataaaa gaaatcagga gatatgtttt acttggacag tcacttgcaa
    33121 tggcaatgaa aagttgcggt tatgacttcc catcgaaatt atgcattaac aaactattac
    33181 ttcattctga aaatgctgac aatgcattga tgatgtctaa ctatgctgat aaatggctgg
    33241 aggaagcaaa aggaaaagtc aaatcaacag gagtctggat aactgtagta tccgcattaa
    33301 ttgttttcgc atttattgta atgatgatta ctgctctcta cgatgtcagt aacgtcctgc
    33361 aacactaatc cctaatattt ttaacctctc aataaataag gaattattta ttatgtcttc
    33421 tattaatatt ttaaatatgc gttctgtttt ttcttctctt tctgcacgcc gtaaaaaaga
    33481 acaggacaaa ggggcaacac tgatggaagt gttattggtt gttggtgtta ttgttgtact
    33541 tgcagcaagt gcatataaac tctattcaat ggtgcaatcc aacattcaat catccaatga
    33601 acaaaacaat gtcctgacag tgattgccaa catgaaatca ctgaaattcc aggggcgtta
    33661 tactgacagt aactatatta agactctcta cgcgcaagga ttactgccat ccgatatgat
    33721 cgcagataca actggcgcaa gcgcgaaaaa cccgtggggc ggctccgtta caatcaccac
    33781 ctcttcggat aaatactcct tcaatgttgt agaggccaat gtcccacaga aaaactgcat
    33841 ggcaatggta aacgccctgc gttcttccag cgcaatctcc aaaattaaca acacttccac
    33901 atcaaccgtc agtgcagcaa ctgtttgcgc atctgacagc aatacgctga ccttcagcac
    33961 tgattcctga tttttcatgt gcatcggata agcgccttcg ggcgcttatt ttttccatgt
    34021 ttaaggagga gtattgtgcg ttctgttctt tatatcctgc tatcatgtat ttcatcatta
    34081 atcttttcat tgcaacccgc atattcagcg acaacgaccc gatttgatgc ctgctttgca
    34141 caagccgggc aacgttacct gattgacccc ctgcttctga aaagcattgc cactgtagaa
    34201 agttcactca taccagaccg aataaatcac aacaaagaaa aaggaaaaat attaagcacc
    34261 gattacggac taatgcaaat taattcatca cacatcccta acctgaaaaa acttggcgta
    34321 ataaaagaca aatcagagct tattgataac ccctgtctca atatacaaat tggcgcgtgg
    34381 attctggcaa cacattttca gaaatgtggc ataaactgga gttgtcttgg cagttataac
    34441 gctggtttca aagaaagtaa cgaacagaaa agaatcaaat acgcacgcta tgtctacaat
    34501 aaatacatgg taaggtaagt tatgctaaac agcatattta atacggcgat actttgctgt
    34561 gcaaatatcg tctgtcaatg ctacgcctac aatgaagtta aattccgttt aaataaactt
    34621 aatgtatctt ataaaaccgg aacagagaaa tattactgtc tgatactgac aacgcttgct
    34681 gttttgtatc tttcattaaa tcagctatcc ctgattcaat ccataatagt gattgttttc
    34741 tatgccttcc ttacattaat ggcctgtata gaccttcttt catttctact accacgcctt
    34801 tataccgtga catttatctt ctccggcctg ttatatcaga catggaataa caatatattg
    34861 tctggtttgt tttgtgcaat gctgatgttc ttcattatgc tgtttgtaag actttacttt
    34921 gcatataaaa acggaacgga aagtttcggc atgggagatg tgctacttat tgcaggaact
    34981 ggcgtatggt ttccaacgcc tgagattgcc tgttcaattg tatttattgc cgttatcggc
    35041 ggaattattt tcttcactct tggcggatta aacaagcaaa aaaaatatat cccattcggt
    35101 ccgttccttt gtggcggaat gtttgtttac tcactcgtac cgggaatatt attctgagga
    35161 ttaagagcaa tgaaaaagac agataaaggt gtatctctcc ttgaggtatt acttgtcatc
    35221 ggcataatgg tgatggttat ccctaaagtt tatgaaaaca ttgaaaacca tctgaataat
    35281 gttcgctggc agaatgcagc agaacacgca aatacctata acaccgctgt tcgtaattat
    35341 gttgctgaca atgcatcaac actacttgct ggctctttac ccaaaacaat cacgccagca
    35401 acactcattc agaaaggcta tctgaaatct ggtttttctg aatccaattt tggacaaagc
    35461 tacattaccg gtattgcaaa gaacagcaaa acatcccgtc tggaagccct gacctgtagt
    35521 aatggcggtc agtccctgtc agaagccgga atgcgctcgg tagcaagcat gattgaaggg
    35581 cttggcggct acatcaacag cagcaagcag gcgattggcg cgggtggcgg ctggtctgac
    35641 acgccttcaa attacgggct gaactgcgcg accgggcata ttgcaatggc acttgttggc
    35701 gctgatttgc aggaaagcga ccgtttatat cgttactcca taaccaatcg tcccgacctt
    35761 aacaggatgc atacggcgat tgatatgaac agcaacaatc tgaacaacgt cggcacttta
    35821 aacggcaacg ccgccgcgct ctccggcgat atcagcgcac gtaacggtac gttctctggc
    35881 gcaatttcag gaaatacagc cacaaccaac ggcgatatca cttcgaataa tggctggctg
    35941 gtcacaaaaa acagcaaagg ctggatgaac agtacctatg gtggcggctg gtatatgtcc
    36001 gattcgtcct ggttgcgcag cgtgaataat aaagggattt atactggcgg ccaggtaaaa
    36061 ggtggaactg tacgcgctga tggacgcctc tatacaggcg agtatttaca gcttgaaaaa
    36121 acagcaacgg ctggcacttc ctgctcccct aacgggcttg taggccgcga cagtacaggg
    36181 gc
//
LOCUS       DAZFTN010000044        35454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFTN010000044 DAZFTN010000000
VERSION     DAZFTN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..664
                     /gene="elfA"
                     /locus_tag="ACHKV2_002333"
     CDS             125..664
                     /gene="elfA"
                     /locus_tag="ACHKV2_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940209.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            747..1448
                     /gene="elfD"
                     /locus_tag="ACHKV2_002334"
     CDS             747..1448
                     /gene="elfD"
                     /locus_tag="ACHKV2_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6940210.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1473..4072
                     /gene="elfC"
                     /locus_tag="ACHKV2_002335"
                     /pseudo
     CDS             1473..4072
                     /gene="elfC"
                     /locus_tag="ACHKV2_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4063..5133
                     /gene="elfG"
                     /locus_tag="ACHKV2_002336"
                     /pseudo
     CDS             4063..5133
                     /gene="elfG"
                     /locus_tag="ACHKV2_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5161..5688
                     /gene="ycbU"
                     /locus_tag="ACHKV2_002337"
     CDS             5161..5688
                     /gene="ycbU"
                     /locus_tag="ACHKV2_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940211.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5696..6211
                     /gene="ycbV"
                     /locus_tag="ACHKV2_002338"
     CDS             5696..6211
                     /gene="ycbV"
                     /locus_tag="ACHKV2_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940212.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6177..6913
                     /gene="ycbF"
                     /locus_tag="ACHKV2_002339"
                     /pseudo
     CDS             6177..6913
                     /gene="ycbF"
                     /locus_tag="ACHKV2_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            7024..8034
                     /gene="pyrD"
                     /locus_tag="ACHKV2_002340"
     CDS             7024..8034
                     /gene="pyrD"
                     /locus_tag="ACHKV2_002340"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO6940213.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8208..8750
                     /gene="zapC"
                     /locus_tag="ACHKV2_002341"
     CDS             8208..8750
                     /gene="zapC"
                     /locus_tag="ACHKV2_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO6940214.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8747..9856)
                     /gene="ycbX"
                     /locus_tag="ACHKV2_002342"
     CDS             complement(8747..9856)
                     /gene="ycbX"
                     /locus_tag="ACHKV2_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO6940215.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10100..12208
                     /gene="rlmKL"
                     /locus_tag="ACHKV2_002343"
     CDS             10100..12208
                     /gene="rlmKL"
                     /locus_tag="ACHKV2_002343"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO6940216.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12220..14127
                     /gene="uup"
                     /locus_tag="ACHKV2_002344"
     CDS             12220..14127
                     /gene="uup"
                     /locus_tag="ACHKV2_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6940217.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14257..15510
                     /gene="pqiA"
                     /locus_tag="ACHKV2_002345"
     CDS             14257..15510
                     /gene="pqiA"
                     /locus_tag="ACHKV2_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO6940218.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15515..17155
                     /gene="pqiB"
                     /locus_tag="ACHKV2_002346"
     CDS             15515..17155
                     /gene="pqiB"
                     /locus_tag="ACHKV2_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO6940219.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17152..17715
                     /gene="pqiC"
                     /locus_tag="ACHKV2_002347"
     CDS             17152..17715
                     /gene="pqiC"
                     /locus_tag="ACHKV2_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO6940220.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17971..18138
                     /gene="rmf"
                     /locus_tag="ACHKV2_002348"
     CDS             17971..18138
                     /gene="rmf"
                     /locus_tag="ACHKV2_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO6940221.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18208..18726)
                     /gene="fabA"
                     /locus_tag="ACHKV2_002349"
     CDS             complement(18208..18726)
                     /gene="fabA"
                     /locus_tag="ACHKV2_002349"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO6940222.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18795..20555)
                     /gene="ycbZ"
                     /locus_tag="ACHKV2_002350"
     CDS             complement(18795..20555)
                     /gene="ycbZ"
                     /locus_tag="ACHKV2_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6940223.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20741..21193
                     /gene="matP"
                     /locus_tag="ACHKV2_002351"
     CDS             20741..21193
                     /gene="matP"
                     /locus_tag="ACHKV2_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO6940224.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21269..22309)
                     /gene="ompA"
                     /locus_tag="ACHKV2_002352"
     CDS             complement(21269..22309)
                     /gene="ompA"
                     /locus_tag="ACHKV2_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO6940225.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22666..23175)
                     /gene="sulA"
                     /locus_tag="ACHKV2_002353"
     CDS             complement(22666..23175)
                     /gene="sulA"
                     /locus_tag="ACHKV2_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO6940226.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23394..24023
                     /gene="sxy"
                     /locus_tag="ACHKV2_002354"
     CDS             23394..24023
                     /gene="sxy"
                     /locus_tag="ACHKV2_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO6940227.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23986..26148)
                     /gene="yccS"
                     /locus_tag="ACHKV2_002355"
     CDS             complement(23986..26148)
                     /gene="yccS"
                     /locus_tag="ACHKV2_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO6940228.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26158..26604)
                     /gene="yccF"
                     /locus_tag="ACHKV2_002356"
     CDS             complement(26158..26604)
                     /gene="yccF"
                     /locus_tag="ACHKV2_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO6940229.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26727..28781
                     /gene="helD"
                     /locus_tag="ACHKV2_002357"
     CDS             26727..28781
                     /gene="helD"
                     /locus_tag="ACHKV2_002357"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO6940230.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28813..29271)
                     /gene="mgsA"
                     /locus_tag="ACHKV2_002358"
     CDS             complement(28813..29271)
                     /gene="mgsA"
                     /locus_tag="ACHKV2_002358"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO6940231.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29367..30029)
                     /gene="yccT"
                     /locus_tag="ACHKV2_002359"
     CDS             complement(29367..30029)
                     /gene="yccT"
                     /locus_tag="ACHKV2_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO6940232.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30202..30615
                     /gene="yccU"
                     /locus_tag="ACHKV2_002360"
     CDS             30202..30615
                     /gene="yccU"
                     /locus_tag="ACHKV2_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO6940233.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30660..30977)
                     /gene="hspQ"
                     /locus_tag="ACHKV2_002361"
     CDS             complement(30660..30977)
                     /gene="hspQ"
                     /locus_tag="ACHKV2_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO6940234.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31035..32225)
                     /gene="rlmI"
                     /locus_tag="ACHKV2_002362"
     CDS             complement(31035..32225)
                     /gene="rlmI"
                     /locus_tag="ACHKV2_002362"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO6940235.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32320..32598
                     /gene="yccX"
                     /locus_tag="ACHKV2_002363"
     CDS             32320..32598
                     /gene="yccX"
                     /locus_tag="ACHKV2_002363"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO6940236.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32595..32924)
                     /gene="tusE"
                     /locus_tag="ACHKV2_002364"
     CDS             complement(32595..32924)
                     /gene="tusE"
                     /locus_tag="ACHKV2_002364"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO6940237.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33015..33674)
                     /gene="yccA"
                     /locus_tag="ACHKV2_002365"
     CDS             complement(33015..33674)
                     /gene="yccA"
                     /locus_tag="ACHKV2_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO6940238.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34082..35101)
                     /locus_tag="ACHKV2_002366"
     CDS             complement(34082..35101)
                     /locus_tag="ACHKV2_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6940239.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35079..35321)
                     /locus_tag="ACHKV2_002367"
     CDS             complement(35079..35321)
                     /locus_tag="ACHKV2_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO6940240.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         9138 a         8418 c         9176 g         8722 t
ORIGIN      
        1 ttaaagattt gtcacttatt ggatttagta tgcttgattt aaatggcgga caattattgt
       61 tattattaag tctaatgtca tttaaaggca ttatcatatt tacgaagaga tcaggatgat
      121 aacgatgaaa aaaagtgtat tgacggcgtt tataactgtg gtatgtgcaa cgtccagcgt
      181 tatggctgct gatgataatg ctatcacgga tggctcagta acatttaatg gtaaagttat
      241 tgctccagct tgtaccctgg tagctgcgac gaaagattcc gtggtgactt tgccagatgt
      301 tagtgccacg aagttgcaaa ccaatggtca ggtttctggc gtgcaaactg atgtgccaat
      361 tgaattaaaa gattgtgata ctaccgtaac aaaaaatgca acgttcacct ttaatggcac
      421 tgcggatact actcagatta cagcgtttgc taaccaggcc tcatctgatg ctgctacaaa
      481 cgtggccctg caaatgtata tgaatgatgg tacaacggcc atcaagccag acacagaaac
      541 cgggaacatt ttgttgcaag atggagatca gacgttgact tttaaagttg attatatcgc
      601 tacggggaaa gcgacttctg gtaatgtgaa tgcggtaaca aatttccata ttaactatta
      661 ttaatagaac tcattaattg ttttattaat tagtacccct ccagtgttat ggaggggata
      721 ttcatatttt ttaagagtga ctatttatga aaacttgcat aacaaaggga attgtgaccg
      781 taagtttaac ggcaatatta ctcagctgtt cgtccacatg ggcggcaggt aaaggcgggg
      841 ttggacttgc agcaacacgt cttgtttatt cggaaggtga agagcaaatt tcactgggtg
      901 tacgtaacac cagtccggat gttccttatc tgattcagtc atgggtgatg accccagata
      961 ataaaaaatc agcggacttt attattacac caccgctatt tgtgctgaat ccggcaaacg
     1021 agaatctgtt acgcattatg tacattggcg cgccgttggc gaaagacaga gaaactcttt
     1081 tcttcactag cgtacgggca gtcccttcaa caacgaagcg ggaagaaggc aataccctga
     1141 agattgccac acaaagcgtc atcaaacttt tctggcgacc aaaaggttta gcgtatccct
     1201 taggcgaggc tccggcgaaa ctgcgttgca cttcgtcagc tgacatggtt acggtcagta
     1261 acccaacacc ttatttcatt accctgacag acctgaaaat aggtggaaag ttagttaaaa
     1321 atcaaatgat ttcccccttt gataaatacc aattctctct gccaaagggt gccaaaaata
     1381 gcagcgtaac gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta
     1441 aatcgtaatt cgtcttcagt taagagagcg agatgtatag aactcaccga caacacagcc
     1501 tgttaagctc tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag
     1561 cgttcaatgc acaagctgaa acctggttcg atccagcctt tttcaaagat gatccctcaa
     1621 tggtggccga tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg
     1681 tcgatattgt tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa
     1741 cgccagagaa ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggcgtga
     1801 atactgatgc atttccggct tttaaacaac tggacaaaca agcgtgtgcg ccattggcgg
     1861 agattattcc ggatgccagc gtaactttta atgtgaataa actccgtctg gaaatttcag
     1921 taccgcaaat tgccatcaaa agtaatgctc gtggttatgt ccccctgaac gttgggatga
     1981 agggatcaac gcgctattac tgggatattc atttagcggg gctaacagta ttcatagcag
     2041 cgcagacagt gattctggcg acagttattt tctgaattta aacagtggcg ttaatttagg
     2101 cccatggaga ttgcgcaaca attcaacatg gagtcgtagt agtggccaaa ccgcagaatg
     2161 gaagaatctc agcagctatt tgcaacgggc ggttattcct ctgaaaggcg aactgaccgt
     2221 aggtgatgat tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct
     2281 ggcgtcagat gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg
     2341 tatcgccaaa agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac
     2401 ttatgtatcg cctggtgctt ttgaaattag tgatatctat tccacgtcgt cgagcggtga
     2461 tttgttagtt gaaatcaaag aagcggacgg cagcgtcaat agctacagcg tacccttttc
     2521 cagcgtgcca ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata
     2581 cagaaccaat agtaatgaac agcaggagag caaatttgcc caggccacgt tgcagtgggg
     2641 cggaccgtgg ggaacgacct ggtatggtgg tggacaatat gctgaatatt accgtgccgc
     2701 catgtttggt ctgggattta acctcggcga tttcggagca atttcgttcg atgtgaccca
     2761 ggcgaagagt acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta
     2821 tgccaaaacg ctcaaccaat tgggtactaa ctttcaattg atgggctatc gctattcgac
     2881 gtcgggtttc tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa
     2941 tgacggtgat gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa
     3001 acgtggcaaa ctgcaggtca atatctccca gcaattaagc gagtacggtt cgttttattt
     3061 aagtggtagc cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg
     3121 ctataacacg caaattaaag atctctcgct ggggatctcc tggaactaca gtaagtcccg
     3181 tggccaacct gatgctgatc aggtgtttgc acttaatttt tccctgccac tcaatctgtt
     3241 gctctccaga agtaatgata gctataccag caaaaaaaat tacgcctgga tgacctctaa
     3301 caccagtatc gataacgaag ggcacactac acaaaacctg ggtttaacgg agacactact
     3361 cgatgacggt aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc
     3421 taatggcagc gccagcatgg actacaaagg ggtgtttgca gatgcccgag tgggctacaa
     3481 ctacagcgat aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc
     3541 ccattcacag ggcattaccc tggggcaatc actgggggaa actaacgttc tgattgcagc
     3601 accaggcgca gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg
     3661 atataccgtt gttccttatg ccacttctta tcgtgaaaat cgaatcgcac ttgatgcggc
     3721 gtcgttaaaa cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg
     3781 ggcgttggtt ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc
     3841 aaagcagggt atatcgctgc gttttggcgc gatagcgacg ctggacggcg tacagactaa
     3901 tagcggcata attgatgatg atggctcgct ctatatggcg ggtttaccgg cgaagggaac
     3961 aataaccgtg cgctggggcg aagctcccga tcaaatttgt catatcagtt acgagcttac
     4021 cgaacaacaa attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga
     4081 gaaaaatgcg tgtcattact acgactattt tttgccgccg tcttaatgct atggtgcgct
     4141 caaaccgctg cttatagcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc
     4201 aattttggcg ttaaaaccct ggaggaagaa gaaaatacga ctggggtagt aaaagacaaa
     4261 ttttatcagt ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat
     4321 gtcagaagtg gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac
     4381 tggtacaaaa ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct
     4441 cctacagcgg ttcctttcga aaacgtgggg actggggcag atacccggtg gcatatttgt
     4501 gaccccggcg gtcaacgttt aggcggccag ggagctagcg gtaatagcgg tagcttttcc
     4561 ctgaaaatat tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga
     4621 ttatttgaat gctacaacat acccgcaggt gattcctgca cgactacagg cacaccggtt
     4681 ttagtgtatt acctgtctgg tactatcaat tcacttggct catgttccgt caatgccgga
     4741 gaaacaatcg aggtcgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat
     4801 aagcctcttg gggccagaac ggcagaactt gcaattccag tcaggtgtaa cacgggaaac
     4861 gcggggttag ttaacgtcaa cctgagtctg acggcaacca cagaccccag ctatccccag
     4921 gcgattaaga cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gaacaacatt
     4981 atttcccctg ctggtggaac attaccgctc tctattcctg atgatgcaga cagtatcgcg
     5041 tgaatgaatg tctatccagt cagcacgaca ggtgtaccac cagaaaccgg gcgatttgaa
     5101 gccacggcaa cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaaacg
     5161 atatttcagt gcgttatctt gttctttagc attcttaaca tccatgtcgg gatggctggg
     5221 cctgaacaag ttagtatgca tatttatggg aatgtggtcg atcagggctg tgatgtcgcc
     5281 accaaaagtg cattacaaaa tattcatatt ggtgatttta atatcagtga ttttcaggcc
     5341 gcgaataccg taagcactgc tgctgatttg aatatcgata tcaccggttg tgccgctggt
     5401 attactggcg cggacgtcct ttttagcggc gaggctgaca cccttgcgcc gacactgctc
     5461 aaactaactg acacaggcgg aagcggtggt atggcaacgg ggattgccgt gcaaattctt
     5521 gatgcgcaaa gtcagcaaga aatcccgctc aatcaggtcc agcctcttac gcccttaaaa
     5581 gccggggata acacactcaa atatcaactt cgttataagt ccacaaaggc gggagcaacg
     5641 ggcggtaatg cgacggcggt tctctatttt gatctggttt accagtgaag gagtgatgtt
     5701 gaaacggata atctggattc tgttcttact gggattaacg ggggggtgtg agctatttgc
     5761 ccatgatggc acggtcaaca ttagcggatc gtttcgccgt aatacatgcg tgctggcaca
     5821 ggatagcaag caaatcaatg tgcagttagg cgatgtctca ctgacacgtt tttctcatgg
     5881 gaattatggc ccggagaaat ctttcatcat taatttgcag gattgcggaa cagatgtcag
     5941 cacggttgac gtcacctttt caggaacccc cgacggagtg cagagcgaaa tgttgtccat
     6001 tgaaagtggt acggatgcag ccagcgggct ggcaattgcg attctggatg atgcgaagat
     6061 attgattccg cttaaccagg cgagtaaaga ctacagccta catagcggta aggtgccatt
     6121 aactttttat gcgcaattgc gacctgtcaa tagcgatgtg cagtcgggta aagtgaatgc
     6181 cagcgcgaca tttgtacttc attatgacta atacgtggaa cagattggcg ctcttgattt
     6241 tcgccgtttt atcgttgctg gtggcgggtg aattgcaggc aggagtggtg gtcggcggaa
     6301 cgcgatttat ctttccggca gacagagaat cgatatctat tttactgact aataccagtc
     6361 aggaatcctg gcttattaac cgtaaaatca accgcccaac gcgttgggcc gggggtgaag
     6421 cgtcgacagt gccagcacca ttactggccg ctccgccact tattctcctg aagcccggta
     6481 cgacaggcac gttgcgcttg ctgagaacgg aaagcgacat cttgcctgtg gatcgcgaaa
     6541 cgctatttga gttaagcatt gccagcgtgc catccggcaa agttgaaaat cagagcgtaa
     6601 aagtggcgat gcgctcggta tttaaactgt tctggcgacc cgaaggttgc cgggcgaccc
     6661 gctggaagct tatcaacaat tacgctggac acggaagtcg cagggtgtac aactcactaa
     6721 cccaacgcct tattacatta acctgattca ggtgagcgta aatggtaaag cgttaagtaa
     6781 tgtgggagtt gtgccgccta aaagccagcg tcagacaagc tggtgtcagg caatagcacc
     6841 gtgtcatgtt gcctggcggg cgataaatga ttacggcggg ttgagtgcaa agaaggagca
     6901 aaatctgccc tgaaacaggt tcggaaaacg tttgcgtttt ttttgccgca ggtcaattcc
     6961 cttttggtcc gaactcgcac ataatacgcc cccggtttgc acaccgggaa tccaggagag
     7021 ttcatgtact accccttcgt tcgtaaagcc cttttccagc tcgatccaga gcgcgctcat
     7081 gagtttactt ttcagcaatt acgccgcatt accggaacac cgtttgaagc actggtgcgg
     7141 cagaaagtgc ctgcaaaacc tgttaactgc atgggcctga cgtttaaaaa tccgcttggt
     7201 ctggcagccg gtcttgacaa agacggggag tgcattgatg cgttaggcgc gatgggattt
     7261 ggatcgatag agatcggtac cgtcacgcca cgtccacagc caggtaatga caagccgcgt
     7321 ctctttcgtc tggtagatgc cgaaggtttg atcaaccgta tgggctttaa taatcttggc
     7381 gttgataacc tcgtagagaa cgtaaaaaaa gcccattatg acggcgtcct gggtattaac
     7441 atcggcaaaa ataaagatac gccagtagag cagggcaaag atgactatct gatttgtatg
     7501 gaaaaaatct atgcctatgc gggatatatc gccatcaata tttcatcgcc aaatacccca
     7561 ggattacgca cactgcaata tggtgaagcg ctggatgatc tcttaactgc gattaaaaat
     7621 aaacaaaatg atttgcaagc gatgcaccat aaatatgtgc cgatcgcagt gaagatcgcg
     7681 ccggatcttt ctgaagaaga attgatccag gttgccgata gtttagttcg ccataatatt
     7741 gatggcgtta ttgcaaccaa taccacactc gatcgttctc tggttcaggg aatgaaaaat
     7801 tgcgatcaaa ccggtggctt aagtggtcgt ccgcttcagt taaaaagcac cgaaattatt
     7861 cgccgcttgt cactggaatt aaacggtcgc ttaccgatca tcggtgttgg cggcatcgac
     7921 tcggttatcg ctgcgcgtga aaagattgct gcgggtgcct cactggtgca aatttattct
     7981 ggttttattt ttaaaggtcc gccgctgatt aaagaaatcg ttacccatat ctaattattt
     8041 cttcgaattc ctctttataa gataatcagg gctttatttt cagccctggt tgttttatat
     8101 tcattgctgt tgcttattta gacattttgt acttttattg ttgaggttat taagcgaagc
     8161 gacaatggat tgtgttgctg cggttttata gggtagggga gaggcagatg cgaattaaac
     8221 cagacgataa ctggcgttgg tattacgatg aagagcacga tcgtatgatg ctcgatttag
     8281 ccaatggtat gctatttcgc tcacgttttg cgcgcaaaat gttgactcca gatgcctttt
     8341 ctcccgcagg cttttgcgtt gacgacgccg cgctctattt ctcttttgaa gaaaagtgcc
     8401 gcgattttaa tttatctaaa gaacaaaaag ccgaactggt gctgaatgca ctggtagcaa
     8461 ttcgctatct caaaccgcaa atgccgaaaa gctggcattt tgtttcccat ggtgaaatgt
     8521 gggtgcccat gccgggcgat gcggcctgcg tttggttgag cgacactaac gagcaagtga
     8581 atttactggt cgttgaatct ggcgagaatg ccgcgctatg cctgctggca caaccctgcg
     8641 ttgttattgc gggtcgcgcg atgcagttgg gcgacgccat taaaatcatg aacgacaggc
     8701 tgaaaccgca ggttaatgtt gacagcttca gcctcgaaca ggcagtctaa cgcgccaact
     8761 taagtgcagt cttcggtaca cagctacagc aaagaatggt gccatcatcg cccattgctg
     8821 attttttcag cggcgtgact tcgccttcta aaagctgaac acggcaactt ccacaaatgc
     8881 ccgcgcggca agaataaggg atacgaattc cctgattttc taattgctcc agcaacacct
     8941 gttggttatt tccacgaaat gcctgtccct gccagtcaat atctacattg gcgtccggtt
     9001 gttgcgtgat gttggcagta tcatcagcgg cagctgcgcc gtaaattttt gccggagccg
     9061 ttgtcagaat ttccacctca tcgccaacgc gaatcacgcc gctattacgg gcaattaaat
     9121 tctgaccaaa atcgacatcg ccgttatcct gggcagtgcg gaaagattgc aatgttttta
     9181 atggttcgcc tgccggatgt ttttgccctt tttctgggct gacggtggtg aaaatacagc
     9241 ggctacaagg tttaaccaca tcaaacacca catcaccaat gcgaatcact ttccaactat
     9301 cttcttccca ggctgacgcg ccggaaacca ccagattggg gcggaattgc tccattttta
     9361 cactggccgg acaacgttgt tggagatcac gtaacgaggc ttcgttagca agaaggtaag
     9421 gatagccatc agcaaatgac aggggtacag tgttgtggcg tttcacgcgc cgggtcattt
     9481 gtggccccac ccagcgtaat tgcacttcgc gggagaaaaa tccacttaac catttgttga
     9541 tcgcttctgg cgcaattcgc gcggtaaaat gtgtgcccca aacttcggtt ggtgcgtctt
     9601 gtgtggcgaa atcagcaaaa cgaacatatg cgctactgct atctggtgcg gtgagatgca
     9661 agccatcatg cacgggtgaa ggagtaaatc gtaccatctg gggaaactgg cgagcggtga
     9721 taaacgtacc gtcaggttcc gtgatcataa agatgcgatc gaaggccaga ccactgacat
     9781 ctgccagagc atgtgtaaga ccaatgccgc gcatcgattt aacaggatga ataaaaagcc
     9841 gggttaatgt cgccacggtg cggtcctcaa atgaaaataa gccctcaact ttatgacatg
     9901 acgcgcttat tagctataat gcgcaacaat tttcttagcg cctgtcccat taggctattt
     9961 tatttgtcag tttggccctg ggttgtgctc gaaatcctca cgtacttgtg tacgctccgg
    10021 tttctccgcg ctgtccatgt ccagactgac tgcaacaatt acgcctactg cggtaggctc
    10081 ttagagtaaa agtgacgata tgaattctct gtttgccagt acggcccgtg ggctggaaga
    10141 gctgttaaaa actgaactgg aaaacctggg ggccgttgaa tgccaggtgg ttcagggtgg
    10201 ggtccatttc aagggcgaca cacggcttgt ttaccagagc ctgatgtgga gccgcctggc
    10261 ctcgcgtatt atgttgccgc tgggcgagtg taaggtttac agcgatttag acctctatct
    10321 cggtgttcag gcgatcaact ggacagagat gtttaatcct ggcgcgacct tcgctgtcca
    10381 cttcagtggt ttgaatgaca ctatccgcaa cagtcagtac ggcgcgatga aagtgaaaga
    10441 cgcgatcgtc gatgctttca cgcggaaaaa tctgccgcgt ccaaatgttg atcgcgatgc
    10501 gccggatatc cgcgttaacg tctggctgca taaagaaacc gccagtatcg cccttgatct
    10561 cagtggtgat ggtttacatc tgcgtggcta tcgcgatcgt gctggtattg cgccgatcaa
    10621 agaaaccctg gcagccgcta ttgtgatgcg atccggctgg cagccaggaa caccgttgct
    10681 cgatccgatg tgtggttccg gtacgttgct gattgaagcg gcgatgctgg cgaccgatcg
    10741 cgcaccaggc ttgcaccgtg ggcgttgggg ctttagcggc tgggcgcagc atgatgaagc
    10801 tatctggcag gaagtgaaag cggaagcgca aactcgcgcc cgtaaaggcc tggctgagta
    10861 tagctctcat ttttacggtt cggacagcga cgctcgggtg attcaacgtg cacgcactaa
    10921 cgcccgtctt gcggggattg gtgaactgat cacctttgag gtgaaagatg tcgcgcaact
    10981 gaccaatccg ctgccgaaag agccgtacgg cacagtgtta agcaacccgc catacgggga
    11041 acgtctggac agcgaaccgg cgctgattgc gctgcatagc ctgctgggtc ggatcatgaa
    11101 aaaccagttt ggtggctgga atctctcttt gtttagtgcc tcgccggatc tgctaagctg
    11161 cttgcagctg cgtgcagaca aacagtacaa ggcgaaaaac ggcccgctgg actgcgtaca
    11221 gaaaaattac cacgttgccg aaagcactcc agacagcaaa ccggcgatgg tagcggaaga
    11281 ctacgccaac cgcctgcgta agaacctcaa aaaattcgag aagtgggctc gtcaggaagg
    11341 gattgaatgt taccgcctgt atgacgccga tctgccggaa tataacgttg ccgttgaccg
    11401 ttatgccgac tgggtggtgg tgcaggagta tgcgccgcca aaaactattg atgcgcacaa
    11461 agcgcgtcag cgtctgttcg atattatcgc tgcaaccatt tcggtactgg ggattgcgcc
    11521 aaacaaactg gtgctgaaaa cccgtgaacg tcagaagggc aaaaatcagt accagaaact
    11581 gggcgagaag ggcgagtttc tggaagttac cgaatataac gctcacttgt gggtgaacct
    11641 gacggattat ctcgatactg gtctgttcct cgatcaccgt atcgcccgtc gtatgctcgg
    11701 ccagatgagc aaaggcaaag atttcctcaa cctgttctct tataccggca gcgccaccgt
    11761 gcatgcggga ttaggcggtg cacgcagcac caccaccgtg gatatgtcgc gtacttatct
    11821 ggagtgggca gaacgcaacc tgcgtctgaa tggcctaacc gggcgtgcgc atcgcctgat
    11881 tcaggccgat tgcctggcgt ggctgcgtga ggcaaatgaa cagttcgatc tgatctttat
    11941 cgatccgcca accttctcta actcaaaacg aatggaagat gcgtttgatg ttcagcgcga
    12001 tcatctggcg ctgatgaaag atttgaaacg tctgctgcgt gcaggtggga cgatcatgtt
    12061 ctcgaacaac aaacgtggct tccgtatgga tctcgacggc ctggcaaaac tgggactgaa
    12121 agcacaagaa attacgcaaa aaacgctctc ccaggatttc gcccgtaacc gccagatcca
    12181 caactgctgg ctgattaccg cagcctgaaa ggaatagtaa tgtcattaat cagtatgcat
    12241 ggcgcatggc tgtcgttcag cgacgcgccg cttctcgata acgcagaact gcatatcgaa
    12301 gataacgaac gtgtttgtct ggtgggccgc aacggcgcag gcaaatcgac gttaatgaaa
    12361 atcctcaacc gtgaacaagg gctggatgac ggtcgcatta tttacgagca ggatttgatt
    12421 gtagcgcgtc tgcaacagga tccgccgcgt aacgttgagg gtagtgttta tgatttcgtt
    12481 gccgaaggca ttgaagaaca agcggaatac ctgaaacgct atcacgatat ttcgcgcctg
    12541 gtgatgaacg acccgagcga gaaaaatctc aacgaactgg cgaaggttca ggaacagctg
    12601 gatcaccaca acctgtggca gctggaaaac cgcatcaacg aagtgctggc gcaactgggg
    12661 ttagatccta acgttgcgct gtcgtcgctt tccggcggct ggttgcgtaa agcggcatta
    12721 ggacgcgcgc tggtgagcaa tccgcgcgta ttgctgctgg acgaaccgac taaccacctg
    12781 gatatcgaaa ccatcgactg gctggaaggg ttcctcaaaa ccttcaatgg gacgattatt
    12841 ttcatctccc acgaccgttc gtttatccgc aatatggcga cgcgcattgt tgatctcgat
    12901 cgcggcaagc tggtgaccta tccgggcaat tacgatcagt acctgctgga aaaagaagaa
    12961 gccctgcgcg tggaagaact acaaaatgcc gagtttgatc gcaaactggc gcaggaagaa
    13021 gtgtggattc gccaggggat caaagcacgc cgtacccgta atgaaggccg cgtacgcgcc
    13081 ctgaaagcga tgcgtcgcga gcgtggcgaa cgtcgcgaag tgatgggtac cgcaaagatg
    13141 caggtggaag aggccagccg ctccggtaag atcgttttcg aaatggaaga cgtttgctac
    13201 caggttgacg gaaagcaact ggtgaaagat ttttctgccc aggttctacg tggcgacaaa
    13261 attgccctga ttgggccgaa tggctgcggc aaaaccacgc tgctgaaact gatgctcggt
    13321 cagcttcaag cggacagcgg gcgtattcac gttggcacca aactggaagt ggcttatttc
    13381 gatcagcacc gcgcggaact ggatcccgat aaaacggtga tggataacct tgccgaaggt
    13441 aagcaagagg tgatggttaa cggcaagcca cgccacgttt tgggctattt gcaggacttc
    13501 ctgttccatc cgaaacgggc gatgacgcca gtacgtgcgc tttctggcgg tgagcggaac
    13561 cgcttgctgc tggcgcgttt gttcctcaaa ccaagcaact tattgattct tgacgaaccg
    13621 accaacgatc ttgatgtcga aacgctggaa ctgctggaag aactgatcga cagctaccag
    13681 ggcacggtat tgctggtaag ccacgatcgt cagtttgtcg ataacaccgt tacagaatgc
    13741 tggatcttcg aaggcggcgg taaaattggt cgttatgtcg gcggttatca tgatgcccgt
    13801 ggtcagcaag agcagtatgt ggcgctcaaa cagcctgcgg tgaaaaaaaa cgaagaaccc
    13861 gccgcgccaa aagcagaaac tgtaaaacgc agcagtagca aactaagcta taaattgcag
    13921 cgcgaactgg agcagctacc gcaattgctc gaagatctgg aggcgaagct ggaagcccta
    13981 cagacgcagg tggcggatgc ttccttcttc agtcagccgc atgagcagac acaaaaagtg
    14041 ctcgccgata tggctgctgc agagcaggag ctggagcaag cctttgaacg ctgggagtat
    14101 cttgaagcgt taaaaaatgg tggctgatcg caaaatagtc gatttaaaca gagacagctg
    14161 acaacggtaa aattgcctga tgaactgggt ttatcaggca tgaaaacaaa cactttgtta
    14221 gtcatctgta aaatagcgca tcattaagga gtaccaatgt gcgaacatca tcatgccgcg
    14281 aagcacatcc tgtgctcgca gtgtgacatg ctggtggcgt taccgcgcct tgagcatggt
    14341 cagaaagcgg catgtccccg gtgtggcaca acgttaaccg tggcgtggga tgccccccgg
    14401 cagcgtccga ccgcctatgc gttggctgca ctgttcatgc tgttgctgtc caacttgttt
    14461 ccttttgtga atatgaacgt tgcaggagtt accagtgaaa ttacattact ggaaattccc
    14521 ggcgtgcttt tttctgagga ctacgccagc ctcggcacct ttttcctgtt gtttgtgcaa
    14581 ctggttcccg cgttttgtct gataaccatt ctgttactgg tgaatcgcgc ggaattaccg
    14641 gtccgtttaa aagagcaact ggcacgggtg ctttttcaac tcaaaacctg gggaatggcg
    14701 gagattttcc tcgcgggtgt gctggtcagt ttcgttaaac tgatggctta cggcagcatt
    14761 ggcgtaggca gcagctttct cccctggtgt ttattttgtg tcctgcaact gcgcgctttt
    14821 cagtgcgttg atcgtcgctg gttatgggac gatatcgccc cgatgccaga actgcgccag
    14881 ccgctaaaac caggcgtcac ggggatacgt caggggctgc gttcgtgctc ctgttgtacg
    14941 gcaatccttc ctgctgatga acccgtgtgc ccgcgctgtg gtactaaagg gtacgttcga
    15001 cgtagaaaca gcctgcaatg gacactcgcg ctgcttgtta cgtccatcat gctgtatctt
    15061 ccggctaata ttttgcccat catggtgacg gatttattag gctcgaagat gccatcgacg
    15121 attctcgctg gggtcatcct gttatggagc gaaggctctt atcccgtcgc tgcggtgatc
    15181 tttctggcca gtattatggt gccaacgtta aagatgatcg ccatcgcgtg gctgtgttgg
    15241 gatgccaaag ggcatggcaa gcgcgacagt gaaagaatgc atttgattta tgaagttgtt
    15301 gagtttgtag gccgctggtc gatgattgac gttttcgtta tcgcggtgct ctcggcgctg
    15361 gtgcgtatgg gaggtttaat gagtatttat ccggcaatgg gtgcattaat gtttgcttta
    15421 gtcgtcataa tgacaatgtt ttctgctatg acgtttgacc cgcgtttgtc gtgggatcgt
    15481 caacctgaat cagagcatga ggagtcctga cagtatggaa tctaataatg gggaagccaa
    15541 aatccagaaa gtgaagaact ggtctcccgt gtggatattt cctatcgtca cggcgctcat
    15601 tggggcctgg gttctttttt atcattacag ccatcaggga ccggaagtga ccctgatcac
    15661 cgcgaatgcg gaaggaattg aaggtggcaa aaccaccatt aaaagccgta gcgttgacgt
    15721 cggcgtggtt gaaagcgcca cactggctga tgatttgaca cacgttgaaa tcaaagcgcg
    15781 gctgaattcc ggtatggaaa aattgctgca taaagatacc gtcttttggg tggtgaaacc
    15841 gcagattggt cgagaaggga ttagcggtct gggaaccctg ctgtctgggg tttatatcga
    15901 actgcagcca ggcgcgaaag gcagcaaaat ggataaatac gatttgctgg actcgccacc
    15961 gttggccccg cctgatgcga aaggtatccg tgtggttctc gatagcaaaa aagccgggca
    16021 gctctcgcca ggagatccgg tgctgttccg tggctatcgg gtaggttcgg ttgaaaccag
    16081 caccttcgat acgcaaaaac gcaatatcag ttatcaactg ttcatcaatg caccttatga
    16141 ccgactggtg accagcaatg ttcgcttctg gaaagatagt ggcattgcgg ttgatctgac
    16201 gtcagcggga atgcgtgtgg agatgggctc attgacaacg ctgctgagtg gcggtgtcag
    16261 ctttgatgtg ccggaaggtc tggatttagg gcagccagtg gcaccgaaaa cagctttcgt
    16321 tttgtatgat gatcagaaga gcattcagga ttcgttgtac accgatcaca ttgattatct
    16381 gatgttcttt aaagattcgg tacgcggtct gcaaccggga gctccggtag aattccgggg
    16441 tattcgcctc ggtaccgtaa gcaaagtgcc attctttgcg ccgaatatgc gtcagacatt
    16501 taacgatgat taccgtattc cggtgctgat tcgtatcgag ccagagcggc tgaaaatgca
    16561 gcttggcgaa aatgcggatg ttgttgagca ccttggcgaa ttgttgaaac gtggtttacg
    16621 cggatcgctg aaaaccggaa acctggtcac tggcgcactg tatgttgatc tcgatttcta
    16681 tccaaatacg cctgcaataa ccggtattcg tgaatttaat ggttatcaga ttatcccgac
    16741 cgttagcggc ggcctggcgc aaatccagca acgactgatg gaagcgttgg ataagatcaa
    16801 caaactgcca ttgaatccga tgattgaaca ggcaaccagt acgctttctg aaagtcagcg
    16861 cacaatgaaa aacctgcaaa cgacgctgga tagcatgaac aagatcctcg ccagccagtc
    16921 gatgcagcag ttgccgacgg atatgcagtc aacgttgcgt gaattgaatc gcagcatgca
    16981 gggcttccag cccggctccg cagcctacaa caagatggtg gcggatatgc agcgccttga
    17041 tcaggtgttg cgagaactgc aaccggtgct gaaaacgctc aatgagaaga gtaacgcgct
    17101 ggtatttgaa gcgaaggaca aaaaagatcc agagccgaag agggcgaaac aatgaaaaag
    17161 tggctagtga cgattgcagc actgtggctg gccggatgca gctccggcga aattaataaa
    17221 aactattacc agttacctgt ggtgcagagc ggtacacaaa gtaccgccag ccagggcaat
    17281 cgcctgttat gggtagagca ggtcgctgta cctgactatc tggcggggaa tggtgtggtt
    17341 tatcaaacca gtgatgtgaa gtatgtgatt gccaacaaca acttgtgggc cagcccgttg
    17401 gatcaacagt tgcgcaacac cctggtagcc aacctgagca cgcaactgcc cggctgggtg
    17461 gttgcctccc agcctctggg aagcgcccag gacacgctca atgttaccgt aacggagttt
    17521 aacggtcgct atgatggcaa ggtcattgtc agtggtgagt ggctgttgaa ccaccaggga
    17581 caactgatca aacgtccgtt ccgtctggaa ggagtgcaaa ctcaggatgg ttacgatgag
    17641 atggttaaag tgctggccgg tgtctggagt caggaagccg cttctattgc acaagagata
    17701 aagcgtctac cttaattata aagatttgta aatataaccg tctccggtat gttgcctgag
    17761 gcggtttttt tgtttctaac gtgcggaaaa atttgttcct cttcacattt tttgtacaac
    17821 cgacatgccc gtgtagctca caaatatgac agtggcgtga attttgcgca ttgacggcag
    17881 ttatgattcg cggtattgct taactgtgat tgcacattta gtaatcactg ttttcttttc
    17941 caccagaaac cagtatgagg gaaacgaggc atgaagagac aaaaacgaga tcgcctggaa
    18001 cgggcacatc aacgtggtta tcaggccggc atcgccggac gctcaaaaga aatgtgtccc
    18061 tatcagacgc tgaatcaaag gtcacaatgg ctgggaggct ggcgagaagc catggcggac
    18121 agggtagtaa tggcctgatt ctgtctcttt aaaaagaaac ctccgcattg cggaggtttc
    18181 gccttttgat actctgtctg attataatca gaaggcagac gtatcctgga acagaccgac
    18241 tttcaggtcg ctggcggtat agatcagacg accatcaacc agcacttcgc catccgccag
    18301 gcccataatc agacgacggt taacaatgcg tttaaagtga atacggtagg tcactttttt
    18361 cgctgtcggc agtacctgac cagtgaattt cacttcgcca acgcccagcg cgcggccttt
    18421 accttcgccg cccagccagc cgaggtagaa ccctaccagc tgccacattg cgtccaggcc
    18481 caggcatccc ggcataaccg gatcgccaat aaagtggcat ccgaagaacc acagatccgg
    18541 attgatatcc agttctgctt caacataccc tttgtcgaag ttaccacccg tttcggtcat
    18601 tttgaccaca cggtccatca tcagcatgtt cggtgctggc aattgcgggc ctttagcgcc
    18661 aaacagttca ccgcgaccag aggcaagaag gtcttctttt gtataggatt cgcgtttatc
    18721 taccatgttc tctgtaagcc ttattttatt gaagcacgca ggatagctaa cacgtgtacg
    18781 ctgaacaagt ccgatcagtt cggaataaac cagttcagcc aacgtaatgg ccatggaaaa
    18841 cggtgacgtc cttcctgttg cgatgcttgc gcgatacgtt cctggatggt ttgcatcagc
    18901 gtcgtttggc cttcgccatc ccacaccaga tttaataata acggcagtgc gtcagtcaca
    18961 tcgtctactg cccagatggt gaatttgcct tcttctaccg ctttcaccag ttcactgtga
    19021 agacttaaat ggcgaacgtt agctgtgggg ataatgacac cttgtttccc ggttaactca
    19081 cgttgctggc aaatagcaaa gaagccttcg attttctcat ttaaaccacc gaccggctgg
    19141 gcgcgaccga actgatcgac tgaacctgtg atagcgatac tctgattcac cggcacatcg
    19201 gcgagggcgc ttatcagggc gcagagttca gccatcgagg cactatctcc atcaacttca
    19261 ctgtatgact gctcaaatgt cagcgatgct gagaagggga tctgttgctc aagctgtagt
    19321 tccgacatca ggaacgcttg catgatcatc atccctttcg catggatatt gccgccaagc
    19381 tccgctttgc gttcgatgtc ggtgaattca ccatcgccaa tatgtacaac gcagctaatg
    19441 cgagaaggtt cgccaaaagc gcgtggatga cccggaaatt caatgaccga aagggcgtta
    19501 atttgcccga tgcgttcgcc ttcggtttca atcaggattt gctcctgaag gatctcatcc
    19561 tgcatacgtt cagcgaggaa accttcgcgc cattcacgct gctgcagcat taagtttagc
    19621 tgctcgccgg agaaggtgtc gccatcacac agggaggcga cctctttaca ctggcggagg
    19681 atccactgcg ggctaagcgg aagtgtttct tgttcaccgg tgtagcgtgc tgcttcgcgg
    19741 ataagtaccg gccaggcatc cgctcccggg gcaggtaagt gattatgtct ggcggtaaat
    19801 gtcacccagc gacaccactg ggttactgac tccgcatcga caatctgcag agtatcttca
    19861 aattcgctat aaatagcctg ctctgaaagc tctggctcca tctcctggaa atcagccaat
    19921 gattcgcgtt cgcctaccag aatgactttc agcttcaatg gcatcgaagg cacagagacg
    19981 gggagagggc gcgactcatc aaacgcaacc cagtcaaaac gctcgcggtt aacgatattt
    20041 ttcagccgca tccacagcag aggttgcgcc agcagtgtac gcaaagagat gatgagaata
    20101 ccgccatttg cctgatgcac cagaccaggc tgcagggtaa tgtcgccatt aaactggcgc
    20161 aggcagccaa agagttgctc cgcttctacc cagtcggcag cgacaacttg cgttaaagtc
    20221 gcaaaattat catctgcact cactgcgtga cgtaagcgga tggagtggcc ggaaacctca
    20281 tagtgaccgc ccaccagttg gcctgcatcg ctttgtagcg tacgcgcggc attggcaata
    20341 agattcagat actcagactc ttccggggcc ttcgccagca taaaagagga ggatgctcgc
    20401 gtatgcagca actgctccag cgcaaattgc agccgcggtt gagtatcact gaataaagga
    20461 tcgttttcgt caatcaaatg tggctgagca aatatttcct gatagctatc ggtatcagga
    20521 accaggtcac gccatgcaag tttcgtaatg gtcaaagttg atgtttttta gtctgttgtc
    20581 aaagccgcga ttataccgta accggcacta cagcacacgt agaaagcacc gacaatactc
    20641 ctggcatggg cgttaaagct cacaggatgg agattctttt cttcactggc ctaaaaagct
    20701 gatattctgt aaagagttac acggtaacat tgagatcgct atgaaatatc aacaacttga
    20761 aaatcttgaa agcggttgga aatggaagta tctggtgaag aagcatcgcg aaggggagtt
    20821 aatcacccgt tacatagaag ccagtgccgc ccaggaagcc gtcgatgtgt tgctctcgct
    20881 ggaaaatgaa cccgtgctgg taaatggctg gattgacaag catatgaatc cggaactggt
    20941 caatcgcatg aagcaaacca ttcgggcaag acgaaagcgc cattttaatg cggaacacca
    21001 gcatacgcgc aaaaaatcga tcgatctgga atttattgtc tggcaacgtc tggctggtct
    21061 tgcgcagcgt cgcggtaaaa cgctttctga aacgattgtt cagctgattg aagatgcgga
    21121 aaacaaagag aaatacgcga ataaaatgtc ttctctgaag caggatctgc aggcattgct
    21181 gggtaaggaa taactgacga aagtcagttc aatttactaa aggcaaaaaa aaccccgcag
    21241 cagcggggtt tttctaccag acgagaactt aagcttgcgg ctgagttaca acgtctttga
    21301 tacctttaac ttcgatctct acgcgacgat ccggagccag gcagtcgatc agtgcagcac
    21361 gctgtttcac gttgtcacag gtgttgccag taaccgggtt ggattcgccc ataccacgtg
    21421 cggagatctt gtctgccggg atacctttgg agatcaggta atcaacaaca gactgagcac
    21481 ggcgctcgga cagaccctgg ttgtaagcgt cagaaccgat gcggtcggtg taacccagaa
    21541 caactacgga accgtctttc ggatccaggt tgctcagctg gctgtacagc tgatccagag
    21601 cagcctgacc ttccggtttc agggttgctt tgttgaagtt gaacagaacg tcagacttca
    21661 gagtgaagtg cttggtctgt acttccggtg ccggagctgg agccggagca actactggag
    21721 ctgcttcgcc ctgaccgaaa cggtaggaaa cacccaggct cagcatgccg ttgtccggac
    21781 gagtgccgat ggtgtgtgcg tcaccgatgt tgttggtcca ctggtattcc agacgggtag
    21841 cgatttcagg agtgatcgcg tactcaacac cgccagcgaa gaccggagaa acgccggtgt
    21901 cgtggttttt accataaacg ttggatttag tgtctgcacg ccataccata ccacccagac
    21961 gagtgtagat gtccaagtcg tcagtgattg ggtaacccag tttagcggtc agttgaacgc
    22021 cctgagcttt gtatgcaccg ttttcaacgc tgcctttgta cggcatacga cctaaccagt
    22081 cgtaacccat ttcaaagcca acatacgggt taacctggta accaccaaaa gcaccagcgc
    22141 ccagttggtt ttcatgggtc gggccattgt tgttgatgaa accagtgtca tggtactggg
    22201 accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg
    22261 tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct
    22321 cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca
    22381 ccgggggatc tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg
    22441 gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg
    22501 aaaaaaaagt cttgtataag gtatgtttaa tcttttttgt cagcgacaat ttacagaaga
    22561 gaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa attagtcacg actgaaagca
    22621 ttggctgggc gacaaaaaaa gttccaggat taatcctaaa tttacttaat gatacaaatt
    22681 agagtgaatt tttagcccgg aaagttgtct cgtggcgtga gaggatgcgc ttaccggacg
    22741 cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag catgctcttc
    22801 ttcagtcaaa tcatctgcca accaaccgat caccacactg taattgcccg tgcgtaaagc
    22861 gcgaaccatt gactccacag tgtggcaagg ggagagctgg ctaatctgca ttactttcgt
    22921 taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg gtgttaacca
    22981 gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt
    23041 catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg tagtgttttc
    23101 cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac gatgtgcata
    23161 gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggatg tacagtacat
    23221 ccagtgacaa caaagatcaa ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt
    23281 gacgggaaaa cataaattaa tcttgccgct taagaataag ttgcctattt tcgtagttaa
    23341 cggatccgtt aatgtgaatc attcttttat gttatgattt taaaaggaat tttatgaaaa
    23401 gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg ggcacaattg
    23461 aataccgatc attgtttggc agttacagcc tgaccgttga cgacacggtg tttgcgatgg
    23521 tttctgatgg tgagttgtat cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaat
    23581 atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc aattactatc
    23641 gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc
    23701 tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg aaagatttgc
    23761 ccaatatgtc ttttcatctg gaagcgattc tcggggaggt ggggattaag gatgtacggg
    23821 cgttacgtat acttggggca aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg
    23881 tgacagaaaa gattctgttt atgcttgaag gtgccattat cggcattcat gaagctgcgc
    23941 tcccggtggc acgccgccag gagcttgcag aatgggctga ctctcttacg ccgaaacagg
    24001 agtttcctgc ggaacttgag taatctggcg ttgcagacga ccaatctcag gcagtaatgc
    24061 aatcaataat ccaacttgtt gtacgaccag aggttctttg ctgtctgccc gtggttcaag
    24121 ttgctgcatc cgctgtttca ggctagctaa tgcctcattg acgcgttctt catcagcagg
    24181 ttgatgatgt aacgcgtcat caacatagca aactgcgtca tcaagaaacg ccagaatttc
    24241 aggattagtt aactgctccc ggtgagcacc gagggctgag atatagctgg taaacgtatg
    24301 gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt tcggctcgct
    24361 ggacatattt gataccaccg acgccagctc agcatcacgg ttgtgtgcat cgcggcgggc
    24421 aatacgatac gccagacggt tatcacgccc ctgatggtat tgctccagta tggcatcgag
    24481 ataccgacag ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc gaaactgcca
    24541 gtcaggccag atgtagctca ctgccgccca cgcaatggca caaccaatca gcgtatcgat
    24601 tacgcgaggt aacgctactt caaaaccttc acccagtaag ttaaaacaca gtagcaccaa
    24661 aagtgtgatg aacatcgttg catgagcgta ttgcacgtta cggaaggcaa aaaagagcac
    24721 gccggtaata accagcagca ccagctgccc ttccagtgat ggcacaaacc acagcacagg
    24781 aatgccaatg gcgataccta ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt
    24841 ggcgttatag tttggctggc agacaaacaa acttgtcagc aagatccaat acccgtgatg
    24901 cattccggtt atctgaatga tggcgtagcc gaagcacaac accagcgaca ttcttaccgc
    24961 atgacggaag agggcggatt ccggcgtgaa gtgacggcta agacgcagcc agatatcact
    25021 caacccgtgc gggctgtcat cagcgagctc attttcgtca ttattatggg gtagtgcctg
    25081 ggcctgttct gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa
    25141 aaatcccagt gttttgagta aatcggcggg tgcgccgtta tcgcgcatcc gctccagcgc
    25201 agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag gctgacgcaa
    25261 caaaatacag cgtgacagtt gctggcacgc ctggccctgc atcgacatca gccgctgaaa
    25321 acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga
    25381 agagctggca cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt
    25441 tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa
    25501 tgtcgccatc agcagaccgt tggcgagagc caaatcgtac agcggtgcct ggctttcatc
    25561 ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca gttgttcata
    25621 gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac agcagatgac caataagtgt
    25681 caggacgttg taccagacgg caccggccag cagatacatc ggctgctgat accagtgctc
    25741 atacagtgat gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt
    25801 tgcatagcgt tgacccagac cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc
    25861 aatcgcaaat agccagggcc agggaaacag caattctact gaggccgagg cgataaaaaa
    25921 gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt
    25981 cagcgccgct gccaccatcc ccagcgttag cggaatcgtc agttttacat cacccagcca
    26041 ccacggaaac gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat acagccaggc
    26101 gctgttccag gtatagcgtt tgagcaaagg acttagcata aaggccatat tagtggatta
    26161 ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta ccgataccac
    26221 gcgacgacca accggccata gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg
    26281 aatgccaatg attgaaatac attgtgcgat gcccgttgca atgtgcatca ggcataacca
    26341 ccagccaaag aaaatcagcc agaaaatatt caataccgta ccgccagtat tcagcagcac
    26401 atttttgcca gccgggttca gttcatcgac atgaatagct tcattgccat aaggcaccag
    26461 agacagttta gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa tcagcgcaat
    26521 actgaccaga gtcgccaaca gccagcccag agtggtggca aatccgccaa gcacaaagtt
    26581 cagaatgttc aaaacggtac gcataaaacc tcgctttact gtggttttca gtaatgggcg
    26641 gcaattgtaa cgtttttttg ggctggagca cgttttctct gacggttaca ctgataagaa
    26701 ataatttcgt gtggatctac agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc
    26761 tggcacagca cccttacgat cgggcggtga tcctcaatgc cgggattaaa gtctccggcg
    26821 atcgccacga ataccttatt cctttcaatc aattactggc gattcactgt aagcgcggtc
    26881 tggtatgggg cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt ctgcacggta
    26941 ccgaatgggg cgagacgcag cgtttttacc atcatcttga tgctcactgg cggcggtgga
    27001 gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca gcaattggat ttgattgcca
    27061 cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa
    27121 tccgccaggc tttgtcggcg ttgccgttgc cggttaaccg actggaagaa ttcgataact
    27181 gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc gctcggttgc
    27241 agcataacca ggcgtatacc gaagccatgc ttaccgagta tgcggatttt ttccgccagg
    27301 tcgagtcttc accgctgaat ccggcgcagg cccgggcagt cgttaatggc gagcattctc
    27361 tgttagtgct ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct
    27421 ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg tttggtcgca
    27481 aagccgctga agagatggac gagcgtattc gcgaacggct acataccgaa gacattaccg
    27541 cacgcacgtt tcatgcgctg gcgctgcata ttattcagca gggcagcaaa aaagttccga
    27601 tagtcagcaa actggaaaat gataccgctg cccgtcatga actctttatt gctgagtggc
    27661 gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag
    27721 aaatgcagtg gtcagtgcca gaaggtaact tctgggatga tgaaaaatta cagcgtcgtc
    27781 ttgcctcacg cctcgatcgt tgggtaagtc tgatgcggat gcacggtggt gcacaggcag
    27841 aaatgattgc cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt atcaagttga
    27901 tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt
    27961 cgggccttat tcatcaggcg attgtgattc tggagaaagg tcgctttatc agcccgtgga
    28021 agcatattct ggttgatgaa tttcaggata tctcgccgca gcgggcagcg ttgttagcgg
    28081 cattacgcaa gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac tggcaggcga
    28141 tttaccgatt cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa aactttggtg
    28201 aaggcgaacg ctgtgattta gacacgactt accgttttaa cagtcgtatc ggtgaggtgg
    28261 caaaccggtt tattcagcag aacccaggcc agctgaaaaa gccgctaaac agcttaacca
    28321 atggagacaa aaaagccgtc acgttattgg atgagagtca acttgacgct ttgctggata
    28381 agctctctgg ttatgccaaa ccggaagagc gcattctgat cctggcgcgt taccatcaca
    28441 tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa atcgacttta
    28501 tgaccattca tgccagcaaa gggcaacagg cggattacgt cctcatcgtt ggcttgcagg
    28561 agggaagtga tggttttccg gctgcggcgc gggagtcgat tatggaagag gcgctactgc
    28621 caccggttga ggatttcccg gacgctgaag aacggcggtt aatgtacgtg gcgctgaccc
    28681 gggcacgtca tcgggtatgg gcactgttta acaaagagaa tccctctccc tttgtggaaa
    28741 tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc
    28801 acctgtgcaa tattacttca gacggtccgc gagataacgc tgataatcgg ggatcagaat
    28861 atcgaccgcg tcgttgaaat gcggcgactg gataatgaag tctgccgttg ccacgttggt
    28921 ggcgaccgga atgttccata ccgtcgccag acgcagcaag gctttcacgt caggatcgtg
    28981 cggcacggca tttagtggat cccagaagaa aatcaataca tcaattttcc cttctgagat
    29041 caatgcgcca acctgctggt caccccccat tgggccactc aacatcgcgt tgacgttcat
    29101 gccggtcgcg cgggaaatta agttaccggt agtgcctgtt gcatacagta cgtgttgttc
    29161 cagtaacggt tgatgccgtt ccacccagct catcagcatt tgtttgcagt gatcgtgtgc
    29221 caccagcgca atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat
    29281 ccgtagttaa ctttcctaca gattactgta agcacttatc gctgcaagat aaagaccgaa
    29341 aaagcctgcg cacaggcaca aaaatctcag gaagatggtt gtttttccgc ccattgcagg
    29401 aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg ttgttctgtg
    29461 agcacctgag actgcggtgg aatagcatcc agctcggaaa cacccgaaag cagcgtactg
    29521 tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga tttgttatag
    29581 cgttcaacct cgacctcata atcaatcgtt tttgccgttg aggtaatggc gagaatatcc
    29641 agctttaccg gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca
    29701 aaatggttag cttcccgctc attctccagg cgaggcaggc gaaaattgac ctggttgatg
    29761 agctgggtat taaaactgac caccagcggt ggggagatat acagccgttc ttcactattg
    29821 gaaagatgaa tcgtcttctc aacgcgaaac actaactgat gcggtccatt atccagttca
    29881 atgctgtccg caccgcgcag cagagagctg gaaaccttct tgccgtcgag taccagaaga
    29941 tcgacatcgg ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa acataatgca
    30001 atcaaggtgg tcacgatgcc ggttttcata ggtagctcct gtcaaaagac cgcccgaata
    30061 ataatgtgtc aaaatttttc tttaatcata cttactaaca tatagacata tttcccgctt
    30121 ttgctctcat tcattcgtat tagctgcatg gttggcatgt cggctttgtc gtacactttg
    30181 tgaaacagtc tggaggaaat aatgaaagaa accgatattg caggcatttt aacgtccact
    30241 cacaccattg cgctggtggg ggcaagcgac aaacccgatc gtcccagcta tcgcgtgatg
    30301 aaatatctac tcgaccaggg gtatcacgtc attccggttt cgccaaaagt tgccggcaaa
    30361 acgctgctgg gccagcaggg gtacggcacg ctggcagacg ttccggaaaa agtcgatatg
    30421 gtcgatgttt tccgtaattc agaagcggcg tggggcgtag cgcaggaagc tatcgccatt
    30481 ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg agcaagcggc agtactggca
    30541 cgggatgccg ggttgaatgt ggtgatggat cgctgcccgg ctattgagat ccctcgcctg
    30601 ggcctggcca aataaaaaat ccccggaagg caaaaacctt ccggggatct gttcagggat
    30661 tagttacgca gacgcggggc ctggagttgt ttgcggatgg tctgcgccag ctcatccatg
    30721 gatggctgtt caggatgttc atcctgcaat tcgctactta gctgggcttc agccaggtag
    30781 gtatgaaccg gtaggccgtt atcgtcctcc atcaccacgt gataccacgg agcggcgcga
    30841 agctcgtcat tcaccgccag ctcatcaggc gacggttcag agagcgaata aaccgggtcg
    30901 atatccacga ccactccgag ataacctaac agggaatggc ggacctgctg gccgataccg
    30961 aatttgctgg caatcatagt cacctcccgg gaaatcttta cctttgatat aagggtaaaa
    31021 ttccacattt caagttacat gacgcgacag gcaaaccctt tcagatatag cccttccgga
    31081 taggtagcga tcaccggatg atcggccgcc tgacggaact gctctataaa ttgtacatca
    31141 cgaccggcat caattgcggc atccgcgatg attttctgaa ataaatcgct ggtcatcaga
    31201 ccggaacagg agaaagtcag gagaataccg ccttcattca gcagctgaat cgccagcatg
    31261 ttgatatctt tatagccacg acacgcgccc atcaactggc ttttattctc aacaaacttc
    31321 ggcgggtcca tcacgataac gtcaaatttt tcaccgcgat cgcgataggt acgcagcaat
    31381 ttaaagacat catcacggac aaactcagcc ttgctcagat ccagtttgtt cagctcaacg
    31441 ttctgccgtg caatatccag tgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa
    31501 ccgcccatca gtgccgatac ggcgaaacca ccggtatagg agaaacagtt cagtacgcgt
    31561 ttattttcaa cgtagcggcg ggtagccagg cggctgtctc gctggtccag gtagtagccc
    31621 gttttgtgtc cgtgctgtat gtccaccagc agcttcatgc catgttcttc aatcggcagc
    31681 agggcaggcg gcaactcacc ggtgacgagg ccctgggtca gctccatccc ttctttttta
    31741 cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg ggtacagcgt ttgcagggca
    31801 ctaattaatg ccgcgcgctg atattctgcg ccagcactca gcagttgcag caccagaaaa
    31861 ttaccgaaac gatcgatagt aatacccggc aggccatcag attctccggc gattaaacga
    31921 tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa
    31981 cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc
    32041 cggatttgcg aagctggcga ataagcgccg cgtgctaacc attttccctg atgatcaaca
    32101 atatcgatgg tttcaccgag gctggcttta ccttccatac gggcaacggc cccggaaaag
    32161 acccacggat ggcgacgaag taatgatttt tcgcgccctt tggctaacac taaacgtaca
    32221 ctcataattt acttttctgt cgatgcaaag aaatgggcgt cattgtccgg agttttccat
    32281 ggatttgcaa cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt ctgcataatt
    32341 gcctgggttt acgggcgagt tcagggcgta ggatttcgct acaccacaca gtacgaagcg
    32401 aaaagactgg gcttaaccgg gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt
    32461 gcctgcggtg aagaagggca ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg
    32521 cgttctgcgc gtgtagaacg ggtgctcagc gagccgcatc atccctcggg ggaattaacc
    32581 gattttcgta tccgttaaat acattttacc ggttttggca ggccagcaat tttggtggct
    32641 tgcttagccg gacctttcgg aaacagtcgg tataaatagc ggctgttacc tttttcttcg
    32701 ccaaatttat tcgccatcgc ttttaccagc atacgaatcg ccggagaagt attgaattcc
    32761 agatagaaat cacgcacaaa acgcaccact tcccagtgtt ctggcgacag cgaaatccct
    32821 tcgttctctg caatcaccac tgccagcggc tcactccact ggctgctttc tttgagatag
    32881 ccttcggtat ccgtttctat ctctttacct tcgaagatca gcataattat tactacctta
    32941 atcagactgc cggcagtgta acaaaaaaca aagccccgca taaagcgagg ctatgaaagt
    33001 gttagcgggt gagattaatc gcggctggcg aagcccagaa tgctcagcag gctgacgaag
    33061 atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt ttcaccgcca
    33121 tgaatgatgt tgctggtttc aaacaaaata gcgccagagg agatcaaaat gaagaccgcg
    33181 ctgatcgcca gatgcagagc aggcagctgc aggaagatat tagcaaccat accaatcagc
    33241 accaccacaa tacccgccat cagcataccg ccgaggaacg acatatcttt gcgggtggtc
    33301 agcacatatg cagagcagca gaagaacact aacgccgttc cgcccagtgc catagcgatt
    33361 acgtcaccca ttccggcaga cagataggta ttcagaattg gtccgaggat ataacccaga
    33421 aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccat tttataggtc
    33481 aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga tggcagcatc
    33541 agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa
    33601 taggtattac gcagcacttt atgggtgcta agcagtgatg tacggtcatg tgaagaacta
    33661 acaatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc agcataatga
    33721 taatgcgtaa gggcacccag tagttttacc catctttacg catttgatct ggaacaggtt
    33781 taacagcgga ttatcaggtc attaagcaaa tataacgccc tgagaatttc ggcaggcaaa
    33841 agaaaaaggg gttagcattt agctaacccc ttatcttatc tggcggaagc gcagagattc
    33901 gaactctgga accctttcgg gtcgccggtt ttcaagaccg aagaaaaaca aataaaaatc
    33961 agtgtaatac gtgaaattgc tgaaaagtta atcaaattta acaccaagaa aaatcaaggt
    34021 attaggaagt tgctcatagc tagtatacca agtctttttg ccagaaattt ttctggattg
    34081 tctacttgct aaccacgagc ggcaaatcca gtgttggtgt gatttttgtt ttacgatcgt
    34141 aaatcaacac ctgattttct gttttatgcc cgctgaaaat ttgtttgtcg cgactgctgc
    34201 cttcgtaatc tgagatcccc ttggctttta tgtcgtgaaa attgcacccg aacggaacgc
    34261 caactttttg ctcggctgcg cgtttagcct gattccacca gttattcagc gttttagcta
    34321 tgactttccc gcctttggtt gtattgatca catattcgca tgtacaggaa gatacatttc
    34381 gggctaactg gatcgctgta cgtaatcgcg gtgaccattc cttgatttgt ttggtgccgg
    34441 ttttgttttg ctcaatgtag atccctttat ccataatatc ctgccatttc aactcaagca
    34501 catcaccgag tcttgccgca cagagatagg aaatctccat tgcaatgcgt aactgtggaa
    34561 ttgcttccgc atatatcgcc gcatactgtt catcggtgat gtaaacagtg cgggctttaa
    34621 gagagaattt tctgactcct ttgcatgggt tattcttcac atacccacgc tcatatcccc
    34681 acccgtatac tcgactcagg cttgccagtt catgatttgc ctgggtcttg ctctcaagcc
    34741 ctcgtttatc catgaaaatt cttacctgct cagttttgac attatcagca agcacttttc
    34801 cgaataccat cagcaacgcc ttctgatgtt gtcgataatc tttttgggtt cggggggcca
    34861 gttctgtaaa tgcaggggag tccataaaca tgtgccacaa tttagctacg gtcattatgt
    34921 tgtggagttt tgctttttcc agttcataat tttgccagac tttagctacg ctggttttcc
    34981 gcactcttcc tagccctata cttcttgtac ttccttcggg tttccacacg taactgtaac
    35041 cattcgatct aacccgcggt ggcagtacat tatctttttt attttttctt ggtcttccca
    35101 ttgttcagcg cctcaaaatc gggttcagca gaaaccagtt cagatgtttt tggcatcgtt
    35161 gtcagaccgt gtggaatatc cctgcggaga actatgggtt cgtttttagg gccgattaca
    35221 aatgggatgc cgtgcagtct taactggtgt tgctgttttg tgtatcgctc gtatttcgtg
    35281 atctctttaa tctctgctgg agatagagtt aattcgtaca tgtggtcacg tgcctttaca
    35341 gcatgaccgc cgccactata gctggtgggc ggcgatatgg gttgaacatc aaaaatcagc
    35401 ctgactcggg agcagttttt gccagatggc tgaaacgtat ttcgcctggt aacg
//
LOCUS       DAZFTN010000045        35425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFTN010000045 DAZFTN010000000
VERSION     DAZFTN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1574
                     /locus_tag="ACHKV2_002368"
     CDS             <1..1574
                     /locus_tag="ACHKV2_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO6940241.1"
                     /translation="SAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYL
                     MTTRVFRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEI
                     ERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKI
                     VRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRI
                     HKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIERNIR
                     HAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINEL
                     GYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANS
                     LCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDS
                     QFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKP
                     MPFSAWDKSGKLVKE"
     gene            1582..2910
                     /locus_tag="ACHKV2_002369"
     CDS             1582..2910
                     /locus_tag="ACHKV2_002369"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6940242.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2980..3309)
                     /locus_tag="ACHKV2_002370"
     CDS             complement(2980..3309)
                     /locus_tag="ACHKV2_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6940243.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3299..3685)
                     /locus_tag="ACHKV2_002371"
     CDS             complement(3299..3685)
                     /locus_tag="ACHKV2_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6940244.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(3911..5236)
                     /gene="rimO"
                     /locus_tag="ACHKV2_002372"
     CDS             complement(3911..5236)
                     /gene="rimO"
                     /locus_tag="ACHKV2_002372"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO6940245.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5449..5832
                     /gene="bssR"
                     /locus_tag="ACHKV2_002373"
     CDS             5449..5832
                     /gene="bssR"
                     /locus_tag="ACHKV2_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO6940246.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5943..7058
                     /gene="yliI"
                     /locus_tag="ACHKV2_002374"
     CDS             5943..7058
                     /gene="yliI"
                     /locus_tag="ACHKV2_002374"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO6940247.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEILDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7055..7681)
                     /gene="gstB"
                     /locus_tag="ACHKV2_002375"
     CDS             complement(7055..7681)
                     /gene="gstB"
                     /locus_tag="ACHKV2_002375"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO6940248.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7927..9129
                     /gene="dacC"
                     /locus_tag="ACHKV2_002376"
     CDS             7927..9129
                     /gene="dacC"
                     /locus_tag="ACHKV2_002376"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6940249.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9176..9934)
                     /gene="deoR"
                     /locus_tag="ACHKV2_002377"
     CDS             complement(9176..9934)
                     /gene="deoR"
                     /locus_tag="ACHKV2_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO6940250.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9992..10588)
                     /gene="ybjG"
                     /locus_tag="ACHKV2_002378"
     CDS             complement(9992..10588)
                     /gene="ybjG"
                     /locus_tag="ACHKV2_002378"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6940251.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10873..12105
                     /gene="mdfA"
                     /locus_tag="ACHKV2_002379"
     CDS             10873..12105
                     /gene="mdfA"
                     /locus_tag="ACHKV2_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO6940252.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(12146..12430)
                     /gene="ybjH"
                     /locus_tag="ACHKV2_002380"
     CDS             complement(12146..12430)
                     /gene="ybjH"
                     /locus_tag="ACHKV2_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO6940253.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12516..13331)
                     /gene="ybjI"
                     /locus_tag="ACHKV2_002381"
     CDS             complement(12516..13331)
                     /gene="ybjI"
                     /locus_tag="ACHKV2_002381"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO6940254.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13331..14539)
                     /locus_tag="ACHKV2_002382"
     CDS             complement(13331..14539)
                     /locus_tag="ACHKV2_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940255.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14623..15159
                     /gene="rcdA"
                     /locus_tag="ACHKV2_002383"
     CDS             14623..15159
                     /gene="rcdA"
                     /locus_tag="ACHKV2_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO6940256.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15334..17019)
                     /locus_tag="ACHKV2_002384"
     CDS             complement(15334..17019)
                     /locus_tag="ACHKV2_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO6940257.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            17289..17666
                     /locus_tag="ACHKV2_002385"
     CDS             17289..17666
                     /locus_tag="ACHKV2_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO6940258.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(17696..17953)
                     /gene="grxA"
                     /locus_tag="ACHKV2_002386"
     CDS             complement(17696..17953)
                     /gene="grxA"
                     /locus_tag="ACHKV2_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO6940259.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            18113..18400
                     /locus_tag="ACHKV2_002387"
     CDS             18113..18400
                     /locus_tag="ACHKV2_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO6940260.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18384..19106
                     /gene="nfsA"
                     /locus_tag="ACHKV2_002388"
     CDS             18384..19106
                     /gene="nfsA"
                     /locus_tag="ACHKV2_002388"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO6940261.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            19167..20069
                     /gene="rimK"
                     /locus_tag="ACHKV2_002389"
     CDS             19167..20069
                     /gene="rimK"
                     /locus_tag="ACHKV2_002389"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO6940262.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            20157..20633
                     /locus_tag="ACHKV2_002390"
     CDS             20157..20633
                     /locus_tag="ACHKV2_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO6940263.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20984..22096
                     /gene="potF"
                     /locus_tag="ACHKV2_002391"
     CDS             20984..22096
                     /gene="potF"
                     /locus_tag="ACHKV2_002391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO6940264.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            22191..23324
                     /gene="potG"
                     /locus_tag="ACHKV2_002392"
     CDS             22191..23324
                     /gene="potG"
                     /locus_tag="ACHKV2_002392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO6940265.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            23334..24287
                     /gene="potH"
                     /locus_tag="ACHKV2_002393"
     CDS             23334..24287
                     /gene="potH"
                     /locus_tag="ACHKV2_002393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO6940266.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            24284..25129
                     /gene="potI"
                     /locus_tag="ACHKV2_002394"
     CDS             24284..25129
                     /gene="potI"
                     /locus_tag="ACHKV2_002394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO6940267.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            25189..25677
                     /locus_tag="ACHKV2_002395"
     CDS             25189..25677
                     /locus_tag="ACHKV2_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO6940268.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            25718..26845
                     /gene="rlmC"
                     /locus_tag="ACHKV2_002396"
     CDS             25718..26845
                     /gene="rlmC"
                     /locus_tag="ACHKV2_002396"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO6940269.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            27125..28468
                     /locus_tag="ACHKV2_002397"
     CDS             27125..28468
                     /locus_tag="ACHKV2_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6940270.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            28490..28810
                     /locus_tag="ACHKV2_002398"
     CDS             28490..28810
                     /locus_tag="ACHKV2_002398"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6940271.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            28822..30303
                     /locus_tag="ACHKV2_002399"
     CDS             28822..30303
                     /locus_tag="ACHKV2_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO6940272.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(30354..31085)
                     /gene="artJ"
                     /locus_tag="ACHKV2_002400"
     CDS             complement(30354..31085)
                     /gene="artJ"
                     /locus_tag="ACHKV2_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO6940273.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(31376..32044)
                     /gene="artM"
                     /locus_tag="ACHKV2_002401"
     CDS             complement(31376..32044)
                     /gene="artM"
                     /locus_tag="ACHKV2_002401"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO6940274.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(32044..32760)
                     /gene="artQ"
                     /locus_tag="ACHKV2_002402"
     CDS             complement(32044..32760)
                     /gene="artQ"
                     /locus_tag="ACHKV2_002402"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO6940275.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(32767..33498)
                     /gene="artI"
                     /locus_tag="ACHKV2_002403"
     CDS             complement(32767..33498)
                     /gene="artI"
                     /locus_tag="ACHKV2_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO6940276.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(33516..34244)
                     /gene="artP"
                     /locus_tag="ACHKV2_002404"
     CDS             complement(33516..34244)
                     /gene="artP"
                     /locus_tag="ACHKV2_002404"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO6940277.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(34462..34977)
                     /locus_tag="ACHKV2_002405"
     CDS             complement(34462..34977)
                     /locus_tag="ACHKV2_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6940278.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         8770 a         8461 c         9057 g         9137 t
ORIGIN      
        1 catcagcggt gccaaataac gcattattcg aaatctattt ttattatccg ggcggtaatt
       61 tattgaacgc atcagataaa cttttttatc tgccgtttgc gttcattatt atcgtattgc
      121 tggtggttta tttaatgacc actcgtgtgt tccgtcggca attttctgaa atgacagagc
      181 tgattaatac gctggcgttt ttgcctgact caacggatca gatcgaggct ctgaaaattc
      241 gcgaaggcga tgcgaaagag attatcagca tcaaaaattc gatcgcggaa atgaaagatg
      301 ccgaaattga acggtcaaat aaattgctct cactgatctc ttacgatcag gaaagtggtt
      361 ttattaaaaa tatggcgatt attgagtcca acaataatca gtatctggct gtggggatca
      421 tcaaactgtg tggtctggaa gccgtggaag ctgtgtttgg tgttgatgaa cgcaataaaa
      481 tcgtcaggaa attgtgtcag cgaattgctg agaaatatgc acaatgctgc gatatcgtga
      541 cattcaatgc cgatctctat ttacttctgt gtcgggaaaa tgtacagaca tttacccgta
      601 aaatagcgat ggtaaacgat tttgacagca gctttggcta ccgcaatctg cgcatccata
      661 agtctgccat ttgtgaacct ttgcaggggg aaaacgcctg gagttacgca gaaaaactga
      721 aactggcgat ttccagtatc cgtgaccata tgttctccga gtttattttc tgtgatgacg
      781 cgaaactcaa cgaaatagaa gagaatatct ggattgagcg taatattcgc catgcaatgg
      841 aaattggcga actattcctc gtctatcaac cgatcgttga tattaacacc cgcgccattc
      901 tgggcgcgga ggcgttgtgc cgttgggtgt ctgcggagcg ggggatcatt tcaccgctga
      961 agttcattac cattgctgaa gatatcgggt ttatcaatga gctgggttat cagattatta
     1021 aaacggcaat gggtgaattc agacatttta gtcagcgtgc atcgctgaag gatgatttct
     1081 tactgcatat taatgtttcg ccctggcagt taaacgaacc gcactttcat gagcgtttta
     1141 ccaccatcat gaaagaaaat ggcctgaagg cgaacagcct ctgtgttgag atcactgaaa
     1201 ccgtgatcga gcgaattaat gaacattttt atctcaatat tgaacaactg cgtaaacaag
     1261 ggttacggat atcgattgat gactttggca ccggtttgtc aaacctgaaa cgtttttatg
     1321 aaattaatcc agacagcata aaggtggact cgcaatttac cggcgatatt ttcggtactg
     1381 cgggaaaaat tgtgcgcatt attttcgacc tggcacgcta taaccggatc ccggtgattg
     1441 cggaaggcgt agagagcgaa gacgttgcgc gcgaattaat caaattagga tgtgttcagg
     1501 ctcaggggta tctgtatcag aaacccatgc cattctccgc ctgggataaa agtggaaaat
     1561 tagtaaaaga gtagtttacg tatgtccaga atcaataagt tcgtacttac agtcagtctg
     1621 ctgattttta tcatgatttc agcagttgcc tgcgggatct acactcaaat ggtaaaggaa
     1681 cgggtgtata gcctgaaaca gtccgttatt gatactgctt ttgcggtggc aaatattgct
     1741 gaatatcggc gtagcgtggc aattgatctt atcaacacgc taaatcccac ggaggaacag
     1801 ctgttggttg gcttacgcac agcttacgcc gactcggttt caccctctta tttgtacgat
     1861 gtcggtcctt atctgatttc cagtgacgaa tgtattcagg taaaggagtt cgagaaaaat
     1921 tattgtgcag atattatgca ggttgtgaag tatcgacatg tcaaaaatac agggtttatc
     1981 tcttttgacg gtaaaacctt cgtctattac ctctatccgg taactcacaa tcgtagtctg
     2041 atatttttgc ttggtctgga gcgtttttct ttactgtcaa aatcgctggc gatggacagc
     2101 gagaacctga tgttctctct gtttaagaac ggtaaaccgg tgaccggtga tgaatataat
     2161 gctaaaaacg ccatcttcac cgtttcggaa gcgatggagc acttcgccta tttgccgacc
     2221 ggattgtacg tatttgcgta taaaaaagat gtttatttgc gggtctgtac attgattatt
     2281 ttctttgccg cattggtggc agtgatatcg ggtgccagtt gcctctatct ggtacgcaga
     2341 gtgattaatc gtggtattgt ggagaaagaa gccattatta ataaccattt tgaacgcgta
     2401 ctggatggcg ggcttttctt ttcggctgcc gatgtcaaaa aactctacag tatgtataac
     2461 tcggcgttcc tggacgacct gaccaaagca atgggtagaa aatcctttga cgaagattta
     2521 aaagcgctgc cggaaaaagg cggttatttg tgcctgtttg acgtcgataa attcaaaaat
     2581 attaacgaca ccttcggtca tttgctgggc gatgaagtgt tgatgaaagt ggtgaaaatc
     2641 cttaaatcac agatcccggt agataaaggt aaagtctacc gcttcggcgg tgacgaattt
     2701 gcggtgattt atactggcgg cacgctggaa gagttactat cgattctaaa agaaatcgtt
     2761 catttccagg tgggaagcat taatttaagc accagtatcg gtgtagcgca ttcaaatgaa
     2821 tgtactaccg tcgaacgctt gaaaatgctg gcggatgagc ggctgtataa gagtaagaaa
     2881 aacggcaggg ctcagattag ctggcagtag aattagaata gtgttagtac ccctgaatca
     2941 aggggtacta atgaatgtgg taaatcattt gcaaacattt tactttgcaa aaacttccgg
     3001 cccttttatt gccaatgtat tcaaaatacg tagtgccggg ccgctcggtt ttatttcatt
     3061 acgttcccat ttcgagacac tttcttttga cattcccatt gtatgtgcca gcacggattg
     3121 tgacatgccg tatcgggtgc ggatggcttt gatctcttca cccgtcattg atttgacttt
     3181 ggctatacgg gcgctgactt cacgggcttt gaccatgacg gcaatggcat caacctcact
     3241 atcagacatc aggcccagtg cattcagttc ctttgcatct tccagcatca tttgtaattt
     3301 atcgctcatt tttcacctca atcaagaagc cagtttcgat caaatcctct atttgcttat
     3361 tgctaaaggc cagaaatgca tttgccattt ttttgtaggc tgccagttca tcatcttcga
     3421 tttctttgct tcctttactg cttagaccac tcttggacca accatcataa aaaaacacat
     3481 gatttgctga tttaaaaaag ataattgttc tggctccacc actctttcct tgttggaggg
     3541 gtaaccgttt tttgatgaca ccgctaccta aatcagcctc ccatatgcct tgcatcactt
     3601 cttcagcggc agccatgaga actttatcgt gaataccaag ttttttcgtg ctttggctga
     3661 atcgccttgc cttatacaca cccatacatc catccttgtg gtaatgttta ctatagtaac
     3721 cattacgctt atttcaagta attgcgatca aaactttaat ttggtgtgaa aaagtgagag
     3781 ttgcctcgag tacgattcat ttttctcgat ttatgcgtca ggggattttg tgcgtaaggg
     3841 taggacatcg ggaagggtag tagaggcaga tcgaagaaca atatccttgt tagatctgcc
     3901 tttagattaa ttaaacccgg ctaccccaca aatcgtactc atcggcgtgc tcgactttca
     3961 cacgcaggat atcacccggc ttaacgttgg tttcaccatt gagataaacc gcaccgtcga
     4021 tttccggtgc atctgccatg ctgcgaccaa tcgcgccttc ttcgtccact tcgtcgataa
     4081 tcaccagaat ttcacggccc actttctctt gcaggcgctc ggcggaaatc tgctgctgca
     4141 actgcatgaa acggttccag cgttcttctt tcacttcttc cggaacctgg tcaggcaggg
     4201 cattggcgtc tgcaccttca accgggctgt atttaaagca gccaacgcga tccagacgcg
     4261 cttctttcag gaagtcgagt agcatctgga aatcttcttc cgtctcgcca gggaagccga
     4321 caataaaggt tgagcgtagg gtcagttccg ggcagatttc gcgccactgt ttgatgcgcg
     4381 ccagttggcg atctacagaa cccggacgct tcatcagttt gagaatgcgc gggctggcgt
     4441 gctgcaacgg aatgtccaga tacggcagga ttttgccttc tgccatcagt gggatgacgt
     4501 cgtccacatg cggataaggg taaacgtagt gcagacgtgt ccagatcccc agtttcgata
     4561 actgttcgca caggctgacc atgctggttt ttaccggctc gccgttgtgg aagccagtac
     4621 gatgtttaac atcaacgcca taggcggaag tatcctgcga gatcaccaga atctctttaa
     4681 cgcccgcatc taccagacgt ttcgcttcac ttaatacttc gccaatcgga cggctcacca
     4741 ggtcgccgcg catagacgga ataatgcaga aggtgcagcg gtgattacag ccttcagaaa
     4801 ttttcagata ggcataatga cgcggcgtca gtttgacacc ttgttctggc accaggctca
     4861 ggaatgggtt gtgtttcggt tttggcacgt agtgatgaac gtgctccaga acctgctcat
     4921 agctatgagg cccggtgatt tccagcactt tcgggtggac ttcgcggatc tgatcttctt
     4981 ttgcccccag acaaccggtc acaattacct tgccgttttc attcaacgct tcaccaatgg
     5041 cttccagtga ttcttgtacc gcgctgtcaa taaagccgca ggtgttgacg atcaccatgt
     5101 ccgcatcgtc atagctcggt accacgtcat aaccttcagt gcggagttcg gtgagaatac
     5161 gctctgaatc gacaaggttt ttcggacagc caagggaaac aaagccgatt ttcggctggg
     5221 gagttacttt gctcatagct taaaaaatat tcagttacag gaaaggtcag ggcagggatt
     5281 ctacagagtt ctggataaaa tttgtatcgc aatctcattc gctggcggag gcgaaggaaa
     5341 tgtaaatttt gttaattcgg cgtgaagaat tgatcctgga cagcattatg ctcaaaaaat
     5401 agccatacta tttaattgca acaaggctgg aaagaggagg atcgaagtat gttcgttgac
     5461 agacagcgaa tcgatctgct gaaccggttg atcgacgcac gcgttgacct cgccgcatac
     5521 gtgcaactga ggaaggcaaa aggatacatg tccgtcagcg aaagcaatca tctacgagat
     5581 aactttttta aactgaatcg cgaactgcac gataaatcgc tgcggttgaa tcttcatctg
     5641 gatcaggaag agtggagtgc tcttcatcat gctgaagaag cattagcgac agccgcagta
     5701 tgtttgatga gtgggcacca tgattgcccg actgttatta ccgtcaacgc cgataagctt
     5761 gaaaattgtc tgatgagctt aacgctgagt atccagagcc tgcagaagca cgccatgctt
     5821 gagaaggcct gaaaactaag ggggagaaag cgtctccccc ttcatgttta agtttttgta
     5881 aaaatgaatt tgttatctcc tccactgact acgctttaag ccagagtcaa tccggaggcg
     5941 ttatgcatcg acaattcttt ttccttgtgc cccttatttg tctttcttcc gctctctggg
     6001 cggctcctgc aacggtaaat gtcgaagtac tgcaagacaa actcgaccat ccctgggcac
     6061 tggccttttt acccgataac cacggtatgt taatcactct gcgcggcggc gagttgcgtc
     6121 actggcaagc aggaaaagga ttatctgcgc cgctttccgg agttccggac gtttgggcgc
     6181 acgggcaggg cggcctgctg gacgtggttt tagcgcctga ttttgctcag tctcgccgca
     6241 tctggttaag ttattccgaa gttggcgatg atggcaaagc cggaactgct gtggggtatg
     6301 gccgcttaag tgatgatctc tcaaaagtga ccgactttcg caccgtcttt cgccagatgc
     6361 caaaactgtc taccggcaac cattttggcg ggcggctggt attcgacggt aaaggttatc
     6421 tttttattgc tctgggcgaa aacaatcagc gcccgacggc gcaggatctg gataaattac
     6481 agggcaaact ggtgcgtctg accgaccagg gcgaaatcct ggatgataat ccttttataa
     6541 aggaatccgg tgcgcgcgcc gagatctggt cttatggcat tcgtaatccg caaggaatgg
     6601 cgatgaatcc gtggagtaat gcactgtggc tgaatgaaca tggcccgcgc ggtggtgatg
     6661 aaattaatat cccgcaaaaa ggcaaaaact acggctggcc gctggcaacc tggggaatca
     6721 actattcagg ctttaagata ccggaagcga aaggggagat cgtcgccggg accgagcaac
     6781 ctgtttttta ctggaaagat tcgcccgccg tgagcggcat ggccttctat aacagcgata
     6841 aattccccca gtggcagcaa aaattattta ttggcgcgct gaaagataaa gatgtcattg
     6901 tgatgagcgt caacggcgac aaagtaacag aagatggccg tattttaacg gacagagggc
     6961 agcgaattcg tgatgttcgc actggacccg acggttattt atacgttctc accgacgagt
     7021 ccagtgggga attacttaaa gttagcccac gcaattagct aacgggaatc atcaccactt
     7081 tgcgcactgc ggggcgttca gtgagttgct gataccagcg ttgcagattt ggacgcggtg
     7141 tccaggtcag gccaacatta aacaaattgt agatgaacgg tgcgatagcg atatcgccca
     7201 caccaaactc gtcgccagag aaccatttta cttttgccag ttccgcatcg agcagggcaa
     7261 acagggcgtc gcactctttg caactggcat caatggcggc ctgatcgcgc tcttccggtg
     7321 gtgttctgac taatcccatc aggatcccgc gatgagcatt actgagtgtc tggtttgccc
     7381 agtccatcca tttttctgct tccgcacgac gtgccggtga gtcgatccac aggcgttttt
     7441 gcccgtactg tgccgccaga tagcggacaa tggcgtttga ttcccaaaga atgagatcac
     7501 tttcgtcgtc acgcaacaac ggcaccagcc cgttagggtt catcgccaga aaatcagcat
     7561 cgtgatttat cccaaactca cggcccgcga gaatttgctc ataaggtagt tccagttctt
     7621 cgagcgtcag caatactttt tttacgttgg ttgaattatt ccgaccccac agcgtaatca
     7681 tactcacccc ttttccgaca ggcagccaca ggctgaacag gactccgatg gtgaagtaaa
     7741 gttaactttt aagcaacagc tggcaaaaaa atgacaccag aagcagcgaa gcggcaggat
     7801 attgcataaa ctttaaaaac tttaccaact tacggtttct ttaagtttgt gtgtgcgtta
     7861 ttaatcaccg aacttatcat acggcgatat aacgtatttt tttgaatgga tactcgggtg
     7921 gcatttatga cgcaatactc ctctctcctt cgtggtcttg cagcgggttc tgcattttta
     7981 ttcctttttg ccccaacggc attcgcggcc gaacaaaccg ttgaagcgcc gagcgtggat
     8041 gcgcgtgcat ggattttaat ggattacgcc agcggtaaag tgctggcaga aggcaatgcg
     8101 gatgagaaac tggatcccgc gagcctgact aaaatcatga ccagctatgt ggttgggcag
     8161 gcgcttaagg ccgataagat taaactcacc gatatggtga cggtcggtaa agatgcctgg
     8221 gcgacgggaa atccggcact gcgtggttca tcggtaatgt tcctcaaacc gggcgatcag
     8281 gtttcggtgg cagacttgaa caaaggtgtg attatccagt ccggtaatga cgcctgtatt
     8341 gcgctggccg attacgttgc cgggagccag gagtcattta ttggtttgat gaatggttat
     8401 gccaaaaaac tggggctgac caacactacc ttccagacgg tgcacggtct ggatgcgccg
     8461 gggcagttca gtaccgcgcg cgatatggca ttgctgggta aagcattgat ccacgatgtg
     8521 ccggaagagt acgccattca taaagagaaa gagttcacct tcaacaaaat tcgtcagcct
     8581 aaccgtaacc gtctgctgtg gagcagcaat ctgaatgttg atggcatgaa gacaggaacc
     8641 acggcaggcg cgggatataa tctggttgct tcggctaccc agggcgatat gcgtttaatc
     8701 tccgtagtgc tgggggcgaa aaccgaccgt atccgtttta atgagtctga gaaattattg
     8761 acctggggtt tccgcttctt tgaaaccgtg acgccaatta aacctgatgc cacctttgtg
     8821 actcagcgcg tctggtttgg tgataagagc gaagtgaatc tcggggcagg cgaagcgggc
     8881 tcagtgacca taccgcgtgg gcagctgaaa aacctgaagg cgagctacac gttaacggaa
     8941 ccgcagctta ccgcaccgct gaaaaaaggt caggttgtcg ggaccattga tttccagctt
     9001 aacggtaaat ccattgagca gcgtccgctg atcgtgatgg aaaatgtgga agagggcgga
     9061 ttctttggtc ggatgtggga tttcgtgatg atgaaattcc atcagtggtt cggcagctgg
     9121 ttctcttaat cttctgataa ccggatggcg cgaaacgtca tccggttata cgtcattaat
     9181 acatcaactt aatacgctgc gtctgcgcgt acttcacata ctcatcttcc gggcaacaat
     9241 cgctcaccac aatatcaaag cgtttcaggt cacccatgcg cgccggacgc accttgccaa
     9301 atttactgtg gtcaacaacc agcacatgct tttgcgccat cgacatggcc cagtgtttta
     9361 ccggcaactc ttcaagatta aaacaggtag cgcctttact gacatgcacg cccgccgcag
     9421 aataaaaagc gatatccggg caaaaattat tcagcgtttg ctgaaaatcg atgggtttga
     9481 aaatggcgtt gctggcgtga aattcaccac cgcacagaaa tgcgcggcaa tggggttttt
     9541 ctttcagcgc cagaaaggta tttagcgaat aacaaacggc ggtaaaaggg atttcattat
     9601 caatcgcttc aataatccac ggcgtggtgg tgccacagtc aaaaaagagg gtctgatcgg
     9661 gttctaccag tgtcgcagcc agttttgccg cccggcgttt ttcttccacc aggcgggatt
     9721 tttgatcgct taacaggtaa tggctggcgc tgcgcggttc cagaacaata tagccgccga
     9781 gcaaaacgac gggcgcactg tggttgttca gatcgcgacg aatcgtcatc tccgaaaccc
     9841 caagcagggc ggcggcgtct ttaagatgta acttatcgct gcgttttaat tcttgcagca
     9901 gctgcccgat acgctcttcg cgacgtgttt ccataatccc tctgaatagt tattgaagcg
     9961 agccgctcaa tactacactt tttagcagag atcagtcacg cacccagcct ttgcggatcg
    10021 gtaatgcaaa acagaagcga taccacgatt gcagacgttg atagagttta tgccccatcg
    10081 cttgccagat aatctgggca ctaaggcagc caatcatacc tgccagcaat ccaccgagca
    10141 tatccagcgg ccagtggacg ccaagataaa cgcgcgacca ggcaatgacg acggccagca
    10201 ccattaaaag tgagccggac cacaggcgat gccagcataa aaatgccagt gcaaaggtga
    10261 aaatcaccgt accgtgatcg ctcgggaatg agtcatccgc cgcatgatgc aggaagttat
    10321 agccgatatt ttcgacaaac ggtcggtcgt gcggaaaaag atgtcccatc gtccaggaca
    10381 ccaacaggct gaccgccagc gcgatagcga tttttatcac cagttgccgt tgtgctgtaa
    10441 gcccccacaa ccaaagtacc gcggccagca gcggcaccac ggtaatcaaa tctttagcaa
    10501 taaaaatcgc caacgagatc atccacggag ccgagtctgg cgtcgcgtta ataagagaga
    10561 atagagagag attcaaattt tccagcataa cttcccgacg caaagtgatt aaaagggagc
    10621 caatacaggc aagtcgttga gaataaagtg caggttaaac tgggtaaagc ggcatcgtct
    10681 tatttccctc aagcggcctg tttacggtgg gtgattgtaa cgggcatagg ttaaataaaa
    10741 cttaaagaaa gcgtagctat actcgcaata atgtaagaat gtgcttaacc gtggtttcag
    10801 ctacaaaatt cgctttctcg ttagctgcgc ttttattaaa ctctgcgcga ttattattgg
    10861 cgaagaaatt gcatgcaaaa taaattagct tccggtgcca ggcttggacg tcaggcgtta
    10921 cttttccctc tctgtctggt gctttacgaa ttttcaacct atatcggcaa cgatatgatt
    10981 caacccggta tgttggccgt ggtggaacaa tatcaggcgg gcattgattg ggttcctact
    11041 tcgatgaccg cgtatctggc gggcgggatg tttttacaat ggctcctggg gccgttgtcg
    11101 gatcgtattg gtcgccgtcc ggtgatgctg gcgggtgtgg tgtggtttat tgtcacctgt
    11161 ctggcaatat tactggcgca aaacattgaa caattcaccc tgttgcgctt cttgcagggc
    11221 ataagcctct gtttcattgg cgctgtggga tacgccgcaa ttcaggaatc cttcgaagag
    11281 gcggtttgta tcaagatcac cgcgctgatg gcgaacgtgg cgctgattgc tccgctactt
    11341 ggtccactgg tgggcgcggc gtggatccat gtgctgccct gggaagggat gtttgtcttg
    11401 tttgccgcat tggcagcgat ctcctttttc ggtctgcaac gagccatgcc tgaaaccgcc
    11461 acgcgtatag gcgagaaact gtcgctgaaa gaactcggtc gtgactataa gctggtgctg
    11521 aagaacggtc gctttgtggc gggggcgctg gcgctgggat tcgttagcct gccgttgctg
    11581 gcgtggatcg ctcagtcgcc gattatcatc attaccggcg agcagttgag cagctatgaa
    11641 tatggtttgc tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg
    11701 cgtctgacct cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg
    11761 attggtctgt tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg
    11821 accgccgggt taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga
    11881 ttaaccctgt ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg
    11941 caaatgctga tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc
    12001 aacggactgt ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt
    12061 atctttttaa aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg
    12121 ctttgtgcga tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa
    12181 ccatcccttc acctacgctg gcaagatggc gaacataagg atgcgggtgg taagccggag
    12241 ctggcgcagg agccacatag acggtttgcg gcacagtcgc gacgctgacc gcttgtggaa
    12301 cgctgactga atcaggaacc acaaccactt tgtatccact cggcacatcg acggtgacac
    12361 tttgcgccat cgccacacca gtaaagccca ttaaaagcgc gcccaacagt agacaatttt
    12421 tcataatcat ttcgcctgaa ataatgcagc gggttaattt ccgcgaatta tgcagccttc
    12481 attcaggtat tgtgttgccg ggcgtgctgc cgtttttatt ggtcaaatgg cgcttcgtgt
    12541 ttaagaactt tatcgatcac atccagtacg ccttcacggt tattggagcc tgcccggtat
    12601 tttgccgctg cgacgaccgc gttgccggca ttttccattg caaaactaaa gcctgcctga
    12661 cgcagcatct caatatcgtt accgccatcg ccaaagacca ccacttcgct gtcgtctatt
    12721 ccccataatt tctgcagttg gcgaaggcca ttggctttat gtacgccggg gataatcaga
    12781 tcgatgctgc cgttgccggt gtggaccggc accataatat cgccgatggc ctcatgtaat
    12841 gttttttgta cttgtggaat cagttcatcg gaaagattca ggccaaactt aaagaagata
    12901 tcttctaagt tgtcaaagtt atcgacgtat tccagacggt gataatacat ttccgccacc
    12961 attttcatgg catcgtcata ttttttgagc gtataggcgc tattttttcc gcaggcaata
    13021 atttccactt ccgggcgcgt cagcaaatgt tccacgacag tagtaaacgc atccttcgac
    13081 agctcgccat taaaaacatc tttgccttca ctcactaccc agccgccgtt ttcagccaca
    13141 aaggcaattt cattagcaat ttcagggaag aaagagatca actgataata ttgattcccg
    13201 ctggcgacca caaagcgaat gccttgtgct ttcatttgct gatactgagc cataaaccgc
    13261 tcacggttat aggttttttg atcgcttaag aaagtaccat ccatgtctac cgcaattaat
    13321 ttaatgctca tcaactattc tccatcgccg tctgcgtttt ggtatcgggt ttggcgacgg
    13381 ctttcgcgac aatagccgcg agaataacca gcgccagtac aaccagcatt gcactacgta
    13441 atccataatg ttcgccgaga tagcccagca gcggcggccc gacgaggaaa gccagataac
    13501 cggtcgttgc taccacactg acgcgggttg gtgcatcggg gccggtatcg ctggcggcag
    13561 aaatggtcag cgggaagccc agcgaggcac ccagtcccca gagtacaaca gacaccccag
    13621 cgacccaggc gctatcgaca aaaataatca gcccaatacc caacgccccc attagcgcac
    13681 tggcccgaac cacggcaacg cgactgtaac ggtcgatgaa ccaaccgcca gtgaagcgtc
    13741 caacggtcat ccccagggta aaaccggcat aaatcagcga gccggaagtg gggctaaaac
    13801 cgtgaccgtc aaccattaat aagggtaacc agtcgttggc agaaccttcg gcaaaggcca
    13861 tcgccagcac cacaacacca atcagcagca actggatatc gcgataaaaa ggtacgcctt
    13921 tttcgccatg ctgggtgcca tcggcagcat ttttgcccgt accgtcaggg attgcctgaa
    13981 tggcgatata tataggcgcg atgcctacca gcgccgccaa taaaatgtgc accgttgccg
    14041 gaacgccaaa ggccgtcagt gccatcccga caccagcgcc tgccagcgtg cccaggctat
    14101 aaaaaccgtg catcatcggc aaaaccgttt tattcatttc tcgctcaacg gcggcacctt
    14161 caacgtttat cgccacttcc gcagaaccaa aacttgcccc aaagacgccg agaccaacgg
    14221 caaagagcag gggcgatgtc agccagagtg ccagacttaa tatcatcatc ccgaccaatg
    14281 cgcaggacat ggtgaccagg atgacattac gtgtcccaaa gcgtttcact aaccacgccg
    14341 agcagagaat accgctcatc gaaccgatcg acagaccaaa gagaacaccg cccatttcag
    14401 cgatcgagac agagagaata tcgcggatag caggcgtacg ggttgcccag gacgccatta
    14461 acaggcccgg caaaaagaag aacataaaca gcgcccaggt tcggcgtttc aatgcattac
    14521 gtgaagaatt tacggtcata gatcacgtca aaataagaag agggaagaca acattagcaa
    14581 ggttgtgtac atttgtacac aattgcagac agaggaaatg acatgcgtcg cgctaacgat
    14641 ccgcaacggc gagaaaaaat tatccaggcc acgctggagg cggtgaaact ttacggaata
    14701 catgctgtta cgcaccgcaa aattgctacc cttgccgggg taccgttggg atcgatgacc
    14761 tactattttt caggaattga tgagttgttg ctggaggcgt tcagcagttt tactgagatc
    14821 atgtcccggc aatatcaggc attttttagc gatgttagtg atgctcaagg cgcatgccag
    14881 gctatcaccg atatgatcta cagctcacag gttgcaacgc cggataacat ggagctgatg
    14941 taccagctct acgcgctggc tagccgaaaa ccgctattaa aaacggtaat gcaaaactgg
    15001 atgcagcgca gtcagcaaac gctcgaacaa tggtttgaac ccggaaccgc ccgcgcgctt
    15061 gatgcgttta ttgaggggat gacgctgcat tttgtcaccg accgtaagcc gctatcgcgc
    15121 gaggagattt tgaggatggt tgagagggtt gcagggtagt agataaattt caggcaacaa
    15181 aaaacccatc aaccttgaac cgaaatggcg gggttgatgg gctccacaaa atggggacat
    15241 caaagaaaag cagtggcact aattaagact gatgccctgc ggaaaagttc tgcggttgtg
    15301 caaaaaaatt tcattttcag ggcaacttca gttttatcct aatcctggcc ataccatgac
    15361 gatgattgtc cctgccagcg tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc
    15421 cagcgccggg atgttactgc gagctgtatc actgatgatc tccattgccg gcgcgcaggt
    15481 gcgtgcgccc atcattgcgc cgaacaacag tgcgcggttc attcgcaata cataagcacc
    15541 gaacaagaaa cagataacca cgggcacaag actgacaatc aatccggcaa tcaacatctg
    15601 accgccaatc gcgcccagac cgttattaat accgctaccg gcgctcagac caacgcctgc
    15661 cataaacacc atcaagccga actctttcac catgcttaat gccccttgcg gaatgtaacc
    15721 gaaggtcggg tggttagcac gcataaagcc cagcataatt ccggcgaata acaacccggc
    15781 agcgttcccc atgccgaaac tgaatgtgct gaactggaag gtgatcatcc cgatcatcag
    15841 cccaataaca aagaaggcgc agaatgccag caggtcagtg acctggctgt gaatcgagat
    15901 aaagccgatg cgatcggcga tggtttttac gcggcgggca tcgccgctga cttgtaaaac
    15961 gtcacctttg ttaagcacga cgttgtcatc aatcggcatc tcaatctggc tacgaatgac
    16021 gcggttaagg aagcaaccgt gatcggtcaa cttcagttgt gcgagacgtt tacctacagc
    16081 gttatggttt ttaacgacca cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc
    16141 gaaaacttct ttaccgttac ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc
    16201 taccaacgct atttcatcgc ccatttgtag cacggcatca ccgtctggat ttgccagaat
    16261 cccgttacgt cgaatacgtt caatgtagca gccggtttgt cgataaatac ccagttcacg
    16321 cagatttttg ccgtcggtcc aggccaccag ctccgggccg acgcgatagg cgcggatcac
    16381 cggtaaataa accttacggt tggcatcagt gtccaggcca cgttcgcggg cgatttgctg
    16441 ggcgctggtc tgtaagtcct gatgctgcaa tttcggcaag taacgcgcac caacaatcaa
    16501 actcaccaga ccgattaaat aggttaaggc atacccgagg ctcagattat ccagtgccag
    16561 tgagagctgc ctgctttcca taccggaatg acgcagtgta tcgccagcac cgaccagaac
    16621 cggtgtcgac gtcatagagc ctgctaacat accggccgtc aggccaatat cccagccaaa
    16681 cagcttacct aaccctaagg cgatcaccag cgcactgcca accatcacca gtgctaacat
    16741 taggtaattt ttcccatcgc gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc
    16801 gacgcagaaa ataaacagca taaagccaag attaagcgca tcggtgttaa tgctgaaatg
    16861 ttgttggcct aataacagcg atacgactaa aacgccaatg gaattaccca gttggatcga
    16921 accaagtcgt aactttccga gacatagccc aagcgcgagg accacaaata ataacagaat
    16981 gtaattccca tttaacaatt cggcgacgtt tatattcacg gaggctaact tcttgtttac
    17041 tagtaagctg ttgaaagaaa tggtaattta cgataatgtt ttttaccaga attcagggcg
    17101 cagattcatt cagcgcacct aaacgatagt aaagtaacaa tatattttac tagtgtaatc
    17161 acattaggta tcaacggcta tatgaattgc gttggcctat attagcatgg aatgcgaagc
    17221 ggctttatct tactgaacgc cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg
    17281 aggaacgagt gaaacataaa caacgttggg cgggggcaat ctgctgtttt gtcctcttca
    17341 ttgtggtgtg cctttttctg gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc
    17401 ctgaaatcgg cttgctgttt ttcattcttc ctggagcagt tgccagtttc ttttcacagc
    17461 gtagagaagt cctgaaacct ctatttggcg caatgctggc ggcaccctgt tcaatgctta
    17521 ttatgcggct gtttttttca ccgacccgct cattctggca agagctggca tggttactaa
    17581 gcgcggtgtt ctggtgtgcg ctgggggcac tgtgtttctt atttatcagt agcttgttta
    17641 aaccacagca cagaaaaaat cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg
    17701 cgtccagatt ctctttcacc catgcagcaa aatcggtata gccgccgata tgttgctgat
    17761 cgacaaaaat ctgcggcacg gtttctacgg gtttacctgc cttttgttgt agatcttctt
    17821 tagtgatccc ttccgcacga atatctacat actgatactg aaaatcatcg cgttcattgc
    17881 tcaatttctc agccagatct tttgcacgca cacagtaagg gcaacccgga cgaccaaaaa
    17941 taacggtttg cattatttct ctcctcatag atttatgcct gtaatgatca cgctaaaatg
    18001 tattcgctga aagtaggttt aacctgttgc attaattgct aaaagctata actgttaaac
    18061 acaatacagt gaaaagtttt agactgaagg ttcactttgc agagggaagc gtatgcgcgc
    18121 gatcggtaaa ttgcctaaaa gcgtgttgat actggaattt atcggaatga tgttgctggc
    18181 ggtggcgctg ctgtcggtaa gcgactccct gtcgctgcct gagccatttt ctcggccaga
    18241 agtgcagatt ctgatgattt ttctcggtgt tttgctcatg cttcccgctg cggtggtggt
    18301 tattcttcag gtggcaaaac gtcttgcccc acagctgatg aaccgtccac cgcagtattc
    18361 acgttcagaa agagaaaaag ataatgacgc caaccattga acttacttgt ggccatcgct
    18421 ccattcgcca tttcactgat gaacccattt ccgaggcgca gcgtgaggcg attattaaca
    18481 gcgcccgtgc gacgtccagt tccagttttt tgcagtgcag tagcattatt cgcattaccg
    18541 acaaagcgtt acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag
    18601 cggcggagtt ctgggtgttc tgtgccgact ttaaccgcca tttacagatc tgtccggatg
    18661 ctcagcttgg cctggcggaa caattgctgc tcggtgtcgt tgatacggca atgatggcgc
    18721 agaatgcatt aaccgcagcg gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc
    18781 gcaataatat tgaagcggtg acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt
    18841 ttgggctgtg ccttggctgg cctgcggata atccggatct taagccgcgt ttaccgtcct
    18901 ccattttggt gcatgaaaac agctatcaac cgctggataa agacgcactg gcgcagtatg
    18961 acgaacaact ggctgaatat tacctcaccc gtggcagcaa taatcgccgg gatacctgga
    19021 gcgatcatat ccgccgaaca atcattaaag aaagccgccc atttattctg gattatttgc
    19081 acaaacaggg ttgggcgacg cgctaaaacc gccacgccga tgtatgatac gcgggctttt
    19141 gaccaggtct gacagagagg tgcagggtga aaattgccat attgtcccgg gatggaacgc
    19201 tctattcgtg taagcggctg cgtgaagccg ctatacagcg cggtcacctg gttgaaattc
    19261 ttgatccgct ttcttgctac atgaacataa atcctgcggc gtcttctatt cactacaaag
    19321 gccgcaagtt accccatttt gacgcagtga tcccgcgtat tggcaccgcc attacctttt
    19381 atgggacggc ggcactgcgc cagttcgaga tgctggggag ctatccgctc aatgagtcgg
    19441 tcgccattgc ccgggcgcgt gacaaattgc gttccatgca actgctggcg cgtcagggca
    19501 tcgacctgcc tgtcacgggc attgcgcatt cgccggatga taccagcgat ttaatcgaca
    19561 tggtcggtgg tgcgccgctg gtggtcaagt tggttgaagg cacgcaggga attggcgtcg
    19621 tgctggcgga gacgcgtcag gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg
    19681 cgcatattct ggtgcaggaa tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg
    19741 ttgttggcga tgaagtggtc gctgcgattg aacggcgggc gaaagagggc gattttcgtt
    19801 ccaatttgca tcgtggcggc gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa
    19861 tcgcgataaa agccgcgcga acgatggcgc tggacgttgc tggtgtggat attctgcgtg
    19921 ctaatcgcgg gccgttggtg atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa
    19981 aaaccaccgg tatcgacatc gcgggtaaaa tgatccgctg gatcgaacgc cacgctacga
    20041 cagaatattg cctgaaaacg ggtggttagt cgcaatcaca ttactgatca tggttttgcc
    20101 tgctcttttt gcgtaagctg tgccggtctt tttatcgaaa gaggttgtac aaaattatga
    20161 catcgctggt cgttcctggt ctggatacgc tgcgtcaatg gctcgatgac ctggggatga
    20221 gtttttttga atgtgataac tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg
    20281 gtgtctttga tgccaaaatc gatctgatcg ataacacgat cctgttttct gccatggcgg
    20341 aagtccgacc ttcagccgta ttgccgctgg cggcggattt atctgccatc aatgccagtt
    20401 cgctgaccgt gaaagcattt cttgatatgc aggatgataa tctgccaaag ctggtggttt
    20461 gccagtcttt atccgttatg cagggcgtaa cctatgagca gtttgcatgg ttcgtgcgtc
    20521 agagcgaaga gcagatttcg atggtcattc ttgaagctaa tgcccatcaa ctgctgttac
    20581 cgactgacga tgaagggcaa aacaacgtta ccgaaaacta tttcctccac tgataactcc
    20641 tttcgagcac gcagtcgctg gtgcagtggc tgcgcgctgc aaaattatct gctgttttta
    20701 accttttctt aaagattatt tcacttctct tgtgtcgatt tggctttatc acatagagca
    20761 aatacgcata aaaatttgtt aaataccgtt ttttaatccg agctatagtc tcaaaccctg
    20821 gctaaagtta ttcttgcgat gcttttatat agcgagcagt gctggccggg agagagttct
    20881 cttttcttac accgcgccga taaaaaatat gcacgtttat tgcatatctt tcagtgtgac
    20941 aacttttgtt cgtttgttaa cgaactttca gaaggaaaga gatatgaccg ccttaaataa
    21001 aaaatggctt tcgggtctgg ttgcgggtgc tctgatggcc gtctctgtcg gcacgctcgc
    21061 ggctgaacaa aaaacactcc acatttataa ctggtctgat tatatcgccc ccgacacggt
    21121 ggctaacttt gaaaaagaaa ccggtattaa agtcgtctac gatgttttcg actctaacga
    21181 agtactggaa ggcaaattaa tggccgggag caccggcttt gacctggtgg tgccgtcagc
    21241 aagctttctt gaacgccagc tgacagcggg agttttccag ccgctggata aaagcaaatt
    21301 gccggagtgg aagaatctcg acccggaact gctgaagttg gtcgccaaac acgatcccga
    21361 caataaattt gctatgccct atatgtgggc gacgaccggg attggctata acgtcgataa
    21421 agtcaaagcg gtgctgggcg aaaacgcgcc cgtcgatagc tgggacttga tcctcaagcc
    21481 tgaaaatctg gaaaaactga aaagctgcgg tgtatctttc ctcgatgcgc cagaagaagt
    21541 ttttgctacc gtgctgaatt atctcggcaa agatcccaac agcactaaag cggatgatta
    21601 caccggaccg gctaccgatc tgctgttaaa gctgcgcccg aacattcgtt atttccattc
    21661 atctcaatat attaacgacc tggcaaacgg cgatatttgc gtcgctatcg gctgggcagg
    21721 tgatgtctgg caggcgtcaa accgcgcgaa ggaagcgaag aatggcgtga atgtctcgtt
    21781 ctcgattcca aaagaagggg cgatggcgtt ctttgatgta ttcgccatgc ctgcggatgc
    21841 caaaaataaa gacgaagcct atcagttcct gaattacctg ctgcgtcctg atgtggtggc
    21901 acatatctct gaccatgtgt tctatgctaa cgccaataaa gcagccacgc cgctggtgag
    21961 tgcggaagtc cgtgataacc cgggtattta tccgcctgcg gatgtccgcg cgaagttgtt
    22021 cactctgaaa gtacaggctc cgaaaatcga ccgtgtgcgc acccgcgcgt ggaccaaagt
    22081 gaagagcgga aaataatccg cagccttaga tgccgggcgg gagcgccact ccctccggca
    22141 attcgcacca acatggtgcg cttgcacaca tttaatgccg gagagcagcc gtgaatgacg
    22201 ctatccctcg cccccaggcg aaaacccgta aggcgctgac cccgctatta gaaatccgca
    22261 atctgaccaa atcctacgat ggtcagcatg cggttgatga tgtcagcctg accatctaca
    22321 aaggtgaaat ctttgcgctg ctgggcgcat ccggctgtgg caagtccacg ctgctgcgta
    22381 tgctggcagg tttcgaacaa ccttcagccg ggcagattat gcttgatggc gtcgatttgt
    22441 cacaggtgcc gccttacctg cgccccatca atatgatgtt tcagtcttac gcgctgtttc
    22501 cgcatatgac cgtggaacag aacatcgctt ttggcctgaa acaggacaaa ctaccgaaag
    22561 cggaaattgc cagccgggtc aatgagatgc tcggccttgt gcatatgcag gagttcgcca
    22621 aacgcaaacc gcatcagctt tccggtggtc agcgacagcg tgtggcactg gcccgaagcc
    22681 ttgccaagcg cccgaaacta ttactgctcg acgagccgat gggcgcgctg gataaaaagc
    22741 tgcgtgaccg gatgcagctt gaagtggtgg atattctgga acgcgtcggt gtgacttgtg
    22801 tgatggtcac ccacgatcag gaagaggcga tgaccatggc ggggcgtatc gccattatga
    22861 atcgtgggaa atttgtccag attggcgaac cggaagagat ctacgagcat ccgactaccc
    22921 gctatagcgc cgaatttatt ggctcggtta atgtctttga aggtgtactc aaagagcgtc
    22981 aggaagatgg tctggtgctt tattcgccgg ggctggtgca tccactgaaa gtcgatgcgg
    23041 atgcttcagt ggtcgataac gtgccggtac acgtagcgct gcgtccggaa aaaatcatgc
    23101 tttgcgaaga gccgcccgcc aatggttgta acttcgcggt gggggaggtg atacacattg
    23161 cctatctcgg cgatctttcg gtgtatcacg ttcgtctgaa aagtgggcag atgattagcg
    23221 cccagctaca aaacgcccat cgtcatcgta aagggttacc gacctggggc gacgaagtgc
    23281 gtttgtgctg ggaagtggac agctgtgtgg tgctgacggt ttaaggagca aagatgagta
    23341 cacttgaacc tgctgcccag tcgaaaccgc cgggcggatt taagctgtgg ttgtcgcagt
    23401 tgcaaatgaa gcatgggcgc aaactggtca ttgcgttgcc gtatatctgg ttgatcttgc
    23461 tgtttctgct gccatttctg attgtcttta aaataagcct ggcggagatg gcgcgcgcta
    23521 ttccacctta taccgaactg atggagtggg ctgacgggca actgtccatc acccttaatt
    23581 tcggtaattt cctgcaactg accggcgatc cgctctattt cgatgcttat ctccagtcgt
    23641 tacaggtggc ggcgatttcg acattttgct gtttactgat cggctatccg ctggcgtggg
    23701 cggtggcgca cagtaagcct tcgacccgta atatcttatt actactggtg atcctgccgt
    23761 cgtggacctc gtttctgatt cgtgtctacg cctggatggg aatattaaaa aacaacggtg
    23821 tgctgaataa ttttctgctg tggctggggg ttatcgatca accgctgacc attcttcata
    23881 ccaatctggc agtttatatt ggcattgttt acgcctacgt gccgtttatg gtgctgccga
    23941 tttataccgc gttgattcgt attgattatt cgctggtgga agcagcgctg gatctcggtg
    24001 cacgaccgct gaaaacgttc tttactgtga tcgtgccgct gactaaaggt gggattattg
    24061 ccggatcgat gctggtgttt atcccggctg tgggcgagtt tgtgatcccg gaactgctcg
    24121 gtggcccgga cagcatcatg atcgggcgcg tgctatggca agagtttttc aataaccgcg
    24181 actggccggt ggcctcggcg gtagcgatca tcatgttgct gctgctaatt gtgccgataa
    24241 tgtggtttca caaacaccag caaaaaagcg tgggagaaca cggatgaata atttaccggt
    24301 agttcgttcg ccctggcgga ttgtgatttt gctgttgggc ttcacctttc tctacgcgcc
    24361 aatgctgatg ctggttatct attcgtttaa cagctcgaag ctggtgacgg tgtgggccgg
    24421 ctggtcaacg cgctggtatg gcgaattatt gcgcgatgac gcgatgatga gtgcggttgg
    24481 tttaagtctg acaattgcgg cctgtgcggc gacggcggcg gcgatcctcg gaactattgc
    24541 ggcggtggtg ctggtgcgct ttggcaggtt tcgcggatca aatggctttg cctttatgat
    24601 caccgcgccg ctggtaatgc cagatgtcat cacgggcttg tcgctgttgt tattattcgt
    24661 cgcgcttgct catgccattg gctggcctgc ggaccgcggt atgcttacca tctggctggc
    24721 gcatgtcact ttctgtacgg cttatgtggc agtcgttatt tcgtcgcgtc ttcgggaact
    24781 ggatcgctcg atagaagaag cagcgatgga tctcggtgcg acgccgctga aagtgttttt
    24841 cgtcattacg ctaccgatga tcatgcccgc ggtcatttct ggctggttac tggcttttac
    24901 tttgtcgctt gatgatctgg tgatcgccag ctttgtatcc gggccgggag ccaccacgtt
    24961 accgatgctg gtcttttcca gcgtgcggat gggggtgaat ccggaaatca acgccctagc
    25021 aacattgatc ctcggcgcgg tcggaattgt cggatttatc gcctggtatc tgatggctcg
    25081 cgcagaaaaa cagcggatac gcgacatcca gtgtgcaaga cgtggctgaa gacactaaaa
    25141 tttgccaacc tggctacata atgccgcgta tgtcgcggca ttgttttcat ggaagacgaa
    25201 acgttgggat tttttaagaa aacatcttca tctcatgctc gcctgaatgt gcctgcgctt
    25261 gtgcaggtgg cggcgctcgc cattattatg atccgtggcc tcgacgtgct gatgattttc
    25321 aatacgctgg gcgtgcgcgg tattggcgag ttcattcatc gcagcgtaca aacctggagt
    25381 ttaacgctgg tctttttaag cagtctggtg ctggttttca ttgagatctg gtgtgcgttt
    25441 tcactggtga aagggcgtcg ctgggcgcgc tggctgtatc tgctgacaca aatcactgcc
    25501 gcaagttact tgtgggcggc ttcgctgggg tacggttatc cggagctgtt cagcattccc
    25561 ggtgaatcaa aacgtgaaat cttccatagc ctgatgctgc agaagctgcc ggatatgctc
    25621 atcctgatgc tgctgttcgt tccctcgacc agtcggcggt tcttccagtt gcaataatgt
    25681 gtataatcgt cgcccctgat gatgtgaagg tcaatgtatg cagtgcgcac tttacgacgc
    25741 gggtcgctgt cgttcctgtc agtggataac gcagccgatt ccagagcaac tctccgctaa
    25801 aaccgccgat cttaaaaatc tgctcgccga ctttccggtt gaggaatggt gcgcgccggt
    25861 gtcaggcccg gaacaagggt ttcgtaataa agccaaaatg gtggtgagtg gtagcgttga
    25921 aaaaccactg ctcggtatgc tgcatcgcga cggcacaccg gaagaccttt gtgactgccc
    25981 gctttatcct gcctcatttg cgcccgtttt tgcggcgcta aaaccgttta tcgcccgagc
    26041 ggggttaacg ccctacaacg tggcgcgtaa gcgtggcgaa ctgaaataca ttctgctgac
    26101 tgaaagccag agcgatggcg gcatgatgct gcgctttgta ctgcgttctg aaaccaaact
    26161 agcacaactg cgtaaggcat tgccgtggtt acaggcacaa ttgccgcagc taaaagttat
    26221 caccgtcaat attcagccgg tacatatggc gattatggaa ggggagacgg agatctacct
    26281 gaccgaacaa caggcgctgg tagagcgttt taacgatgtg ccgctgtgga tccgtccgca
    26341 aagtttcttc cagactaatc cggcggtcgc cagccagctg tacgctaccg cgcgcgactg
    26401 ggtacgacag ctgccggtta aacatatgtg ggatctgttc tgcggtgtgg ggggctttgg
    26461 tttacactgc gcgacgcctg acatgcagtt aaccgggatc gaaattgcgc tagaggccat
    26521 tgcctgtgca aagcagtcag ccgctgaact gggcttaacg cgtctgcaat ttcaggcgct
    26581 ggactccact cagtttgcca ccgctcaggg ggaggtgccg gagctggtgc tggttaaccc
    26641 gccgcgccgc ggcattggta aaccgctgtg tgattatctc tcaacgatgg caccgcgttt
    26701 tattatctac tccagctgta acgcccaaac catggcgaaa gatatccgcg aactgccagg
    26761 ttaccgcatc gaacgggtac agctttttga tatgttcccg cacaccgcgc actatgaagt
    26821 gctgacgctg ctggtaaaaa tgtaaataca tcattcaaat caatatcacc tcggcataca
    26881 tccatccaaa ataataatcg tgatcgatcg attaataata accgatcgat cacggttata
    26941 tattgctcta tttttgcaaa tcaaatcaca aataatcaaa taagatcttt aattgaactg
    27001 ttttgattac tacagtgcac ttaccgaata catacagagg cattgcttag cgaaacacaa
    27061 aatttcctga gacattatta acgtcagcag aaggttcatg cattgacaac ggaaatgtac
    27121 gcgtatggag ggcgttccga cgatgtttgc caaattcata gatgttattc aaacgttttt
    27181 aactgaaccg gcaattttaa ttggtttgct ggtgggtatt ggttatgccc ttgataagaa
    27241 atcgccaata aaaattatta ccggcatggt cagcgccatg gtgggattaa tgatggtgtt
    27301 atttggtggt ttccagtttt ccgcaacatt taaaccggta gcagaagccg taagtaaagc
    27361 ctatggtgtt cacggatatt taatggattc ctacgcaatg aaagcggcaa cgcaaattgc
    27421 attgggggat aattttggtt atgtcgggta tgtatttgta ctggcgtttt ttaccaacct
    27481 gctgctggta ttatttggtc ggtatactgg tgccaaaggg atatttttga cagggaatac
    27541 cggtgtttca cattctcagg cggtattatg gttgattgtc ttttggctgg gttttagctg
    27601 gactacatcc attattattg ccggaatatt aacgggtgta ttctgggcgt tctccaccac
    27661 gctcattgtt aagcccattg ccaaagttac caaagatgcc ggttttacca ttgctcataa
    27721 ccagatgttg ggattgtggt ttttctccaa attcgcccat aaatttggtg accctgaaaa
    27781 gcacgatgct gaaaacctga aacttcccgg gtggctggcg atttttaacc ataacgtaac
    27841 ggctatcgcc attgtgatga cgctgtttgt cggtggtttc ttgctatcaa cgggtatcga
    27901 taatgtccag ttaatggcga aaggcaagcc ctggtacatc tatatcatca accttggctt
    27961 acagttctcc atgtatatgg tcattctgct gcagggtgtg cgcatgatgg tgggcgaaat
    28021 taacggttcg tttaaaggct ggcaagatcg ctttattcct aacgctattc ctgctgtcga
    28081 tgttgcagcg ctgttacctt tttcacccaa tgccgcaacg ttaggcttcg tcttctgtac
    28141 ttttggcacc attttttcga tggggatctt attactggtt cacagcccaa ttatggtatt
    28201 gcctggcttt gtacctctgt ttttctctgg cggtccaatt ggcgtattag caaaccgcat
    28261 gggcggatac cgttccgtaa ttatctgtac gttcttgctg ggtattattc agaccttcgg
    28321 tacggtgtgg gctattccgt taaccgggct tgctgaaaat ggcgtcggct ggacaggaat
    28381 atttgactgg gcaaccgtat ggcctgctat ttgtgaagtt ctgaaattta tcgctgcaac
    28441 attccatctt ggtccttacg cgggttaatt aaatattaag ggtttaaaaa tgaaagatac
    28501 aattaatatt ctgtttgtct gtggttacgg tgtaggcagt agcgttatgt tacaaacggt
    28561 cgttaaaaaa gcgctgacca aatatgattt caaagttgaa atggaacata ccgccgcagg
    28621 tgaagtcggc ggatttaccg actgggcgga tatttttgct atttccaaaa aattactcga
    28681 tgtggttagt cttgacccga aacaagggca atatttgatt ccgatagaga atattatgga
    28741 cggtgaaact attggtcagc aaatttatga agtggttcag caacattttc cgcatttaat
    28801 taatcaataa ggtagggcgt aatgagagcg attattctgc tgttcgatag tctgaataaa
    28861 cgttatctgc caccttatgg cgatgcgtta accaaagcgc ctaatttcca acgtctggcg
    28921 gctcatgccg ccacctttga aaacagttac gtcggcagta tgccgtgtat gcctgcccgg
    28981 cgggagttgc ataccgggcg atgtaacttc ctgcatcggg agtgggggcc gttagaaccg
    29041 tttgacgatt ccatgccgga gctgcttaaa aaggcgggga tctataccca cctgataagc
    29101 gatcatttgc attactggga agatggcggc gggaattatc ataatcgcta cagttcgtgg
    29161 gagattgtgc gcggacagga aggcgatcac tggcatgcaa gcgttgcgca accgcctatt
    29221 cccgaggtgc tacgggtgcc gcaaaagcag accggtggcg gtgtttctgg tctgtggcgc
    29281 catgactggg caaaccggga atatattcag caggaagcgg attttccaca aactaaagtg
    29341 tttgatgcag gctgtgcctt tatccacaaa aatcacgcgg aagataactg gttattgcag
    29401 atcgaaacgt ttgatccgca cgagccgttt cacacaacgg aagagtatct ttccttgtat
    29461 gaagataact gggatggacc gcattatgac tggccgcgtg gccgggtgca ggaaagcgac
    29521 gaggccgtgg agcacatccg ttgccggtat cgttcgctgg tgtcgatgtg cgatcgcaac
    29581 ctggggcgca ttctcgacct gatggatgag cacgatctgt ggcgagatac catgctgatt
    29641 gtcggcactg accacggctt tttactcggt gagcatggct ggtgggcgaa aaaccagatg
    29701 ccttattaca acgaagttgc caataatccg ttgtttatct ggaacccgcg cagtggtgta
    29761 aaaggagagc ggcgacaggc actggtacaa atgatcgact gggcgccaac gttgtatgac
    29821 ttttttcaac agccagtgcc gcccgatgtg caggggcaac cgctggcgaa aacggtcagt
    29881 cacgatgaac cagtacgcag ctcggcgatg tttggtgttt tcagtggtca tgctaacgta
    29941 actgatgggc gttatgtgta tatgcgtgca gcgctgccgg ggcgtgagga tgatattgcc
    30001 aactacacgt tgatgtcctg caaaatgaac agccgctatc cggtggatga gatgcgggct
    30061 ttatcgctgg ccccaccgtt tcgttttacc aaagggttac aggtattacg catcccggca
    30121 caggaaaaat ataaggggtt gaatcagttt ggtcatttgc tgtttgatct gcaaaacgat
    30181 ccgcagcagc tacatccgat tcatgatgat gtgatcgagt cccggatgat cgcgttgctg
    30241 attcagttga tgaaagataa tgatgcgccg ccagagcagt ttcagcgcct gggattagcg
    30301 tagagaagtg attttgccgg agtgggtgtt cgcccactcc ggcaaatgaa taattattgc
    30361 gggaaccact gatcgctgat ttgttgatat gtaccgtcag ctttaattgc tgccagcgcg
    30421 ttattcagtt tttccagcag agctttgtta tccggacgta cagcgatacc cagaccggtg
    30481 ccaaagtatt gcggatcggt cactttttca gtagcaacgc ccagttgcgg attggttttc
    30541 agccattcat taaccaccgc agtgtcacca aatacgccat caatacggct atttttcaga
    30601 tcgataaagg cattttgata actgtcatag gagaccgttt tcacttccgg gtgctgatcc
    30661 tgaatatatt tctggtgcgt agtaccgttt tccatcccaa tgcgtttgcc tttcaggtcg
    30721 gcaaacgttt tgtaggtatc ttttttggca atcacgacgg ctgagttttc gtaatagggc
    30781 gtggtgaacg atacctgctt gctacgatct ggtgtgatat ccataccgga gattacggcg
    30841 tcatatttcc tgaatttcag tgacgggatc aggctgtcga acgcgtgatt ggtaaaagta
    30901 cattctgcct gcatctgttt gcataacgct tttgctaggt cgatatcaaa gccgacgatc
    30961 tggttattgg catccatcga ctcgaacggc ggataggtcg ctgatacgcc aaaattgatt
    31021 ttctctgcgg cagaagcgcc gaacgcacag gatgccagta atgcggcaag aactaacttt
    31081 ttcatgatgg aactcccgtc tgtcaatctt atgattattg gccgtgtctg cggcatggga
    31141 taacaatgcc atcaagtgaa tttatatgca ataaatatga ttaaataatt tgcatgaaat
    31201 aaaaaaagac ggacaactta gtgagttgtc cgtctttatt ataagaattt atgcactatg
    31261 taggccggat aaggcgtcca cgccgcatcc ggcacaggca ccgtgctgat gtctgatgcg
    31321 acgctggcgc gtcttatcag acctacaaaa cccccggcga atgtacgcag ccacattaat
    31381 tccttcgctc aaacgccagc gctttgcgct cgatcagacg catcatcagc gtcagcaggc
    31441 cgttaacgac cagataaata atccctgccg cgccgaacac catgacatcg taggtgcgtc
    31501 cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtaa gccagagagg
    31561 tacttttgaa caccagcacc acttcgttgg aataagaaga gagcgagcgt ttaaaggcat
    31621 acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta caggactgcc
    31681 actgaccttc cgggatcgca cgaattgcac cgtaaaacag ctgcgtggta tacgccgcac
    31741 tgttcagcga caacgcaatc agcgcacata accacggttc tgacaacaaa tgccacagtg
    31801 ccggatactc ctgcaacgtc gggaactgac ccggcccgta ataaatcagg aagatctgca
    31861 ccagcagcgg tgtaccggta aacagcgtga tataaccccg caccagccac accagcaccg
    31921 gcgttttcag cgtcaggatg atggtaaaaa tcaacgccag aatcagtgcc acaatcagcg
    31981 aggcaacggt tagcgtcagg ctggtgtgca gccctttcat cagttcgggt aaatactcaa
    32041 acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt tgagaatgta
    32101 ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc aggtaaatgg
    32161 ttcctgggta cgagtagcga tgcttttggt ttgcagcatt aaatcattca cactaatcaa
    32221 actgaccagc gcggtatctt tcagcagcac cagccactga ttaccgaggc caggcagcgc
    32281 atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg acagccccag
    32341 cgcctggccg gattcccact gacccatcgg caccgctttc aacgcgccgc gcagcgtttg
    32401 cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga acgggctaac
    32461 gtcgaagttt tcaatctgca tctgcaccgg gatctctaca atcccaagat tgatagtgaa
    32521 gccatccgaa agtgtcagca gcagctgcga ggagccaaaa tagataaaca gcaccaccag
    32581 aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg cgacaggacg
    32641 ccatttcgca gactcccata ccgcaaagaa catcgccagc gccagcccga caatcaatgc
    32701 acaaacggca aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa attcattcat
    32761 caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat ctttcttcac
    32821 tttttccagc gcagtgttga gtttctgctg cagctcagtg ttgccctgac gtaccgcgat
    32881 gccgaggcca gtaccgaagt aatctttatc ggtcacttta tcgcccactg ccgccagttt
    32941 cgggttatct ttcagccatt cagttaccac tgcggtgtca ccaaatacgc tgtcgatacg
    33001 accgttttgc agatccagtt ttgcgttctg gtagctgtca tacggcacgg tggtgatttc
    33061 cgggtgctta tccataatga atttctggtg agtcgtacca ttctgtacgc cgactttttt
    33121 gcctttcagc tgatcaacgc tggtgtattt accctgctga cccacaaaca gggcagagtt
    33181 gtcatagtac ggcgtggtaa acagtacctg cttttcacgt tccgcggtga tatccatccc
    33241 ggccatcacc gcttccacgc gacggaattt caggcttggg atcaggctgt caaatgcctg
    33301 gttagagaaa gtacaggtcg catcaatctc tttacacagc gcctgtgcca ggtcgacgtc
    33361 aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg aggcttcggt
    33421 agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa ttaacgcggc
    33481 aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata gtttttaaat
    33541 gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat atgaccgttt
    33601 tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg ggtgacgatc
    33661 acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg tgccgtaatt
    33721 tccgggtcca gtgcggcggt cggttcatca aacagcagta cctgcggttc catcattaac
    33781 gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg gtaacgatcg
    33841 ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag cgcctgatct
    33901 ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac ggtcagatgc
    33961 ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc gcgaatcgct
    34021 ttgtctgagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag cgtaccggag
    34081 cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc gccgctgggg
    34141 ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa cagcgcctga
    34201 tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac tcgtatactg
    34261 gcagtctgat agctattgag gtcgaagata gtacctttga cagaataatt atgcaatatt
    34321 tctgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca aaataacggg
    34381 ggcggtggtc ggcgagcata aatgtcggca ttcctcacga aatgccggac aatttacggg
    34441 gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg tacccgccgt
    34501 ggcatgaacg ctaccgccta agtagcgcac atcgtcgatc acccaacact ggccttcctg
    34561 aatcattaac acttcatcct gccagccctg gtcaccctgc ttgagatcca cgcgcaatgg
    34621 aatgttacgg gcatcacgat tagggatagt cgatgcactg gcaacgtggg cgctgtctgg
    34681 caaggtggtt cgactggaga acggatcact gcttaacaat tcacgatgac tattatcgcg
    34741 gctggcatcg ctaagtaggg ttgccagttt gtcactcaaa taggggcgca gggcggtaat
    34801 gtcattgctg cgatgttgaa tgtggtagtc atagaattgt tgggccacgc tgtcaggccc
    34861 cccctggaca caggaaccgg tgcggggacc attatcttta taagctggcg tgacggtggt
    34921 acaggcactc agcagcagtg cacatggaat aagtagagat agtgaacggt agcgcataat
    34981 catttcctta ttagcttgct gtctgcgtca cactacgacc aaacgctatc agattattta
    35041 atacattgtt tttaatacaa ttctgaaaca atctgaacac ctgattgggt ttgagtccat
    35101 tgctggcgac ccactcaagg ttgctcgtag ctgacttact taaattaccc aaacaataga
    35161 cagtgtaaac catttgtgag gttgagcgtg ttatatggct gaaatgatca tttaaggtaa
    35221 atatgatagt tctgaaatgg tggtataaat cctcctgtag aggttaagag catctgcaag
    35281 aagccagttt cacatttata acgcgtgata aatgaacgcg gttattgtct aatcattaca
    35341 aatttttcac atgcgataag agatcctgct attaagatga ttgccaaaat tacgttgtat
    35401 ttttgaaaat gagcgaaatt tgata
//
LOCUS       DAZFTN010000046        35248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFTN010000046 DAZFTN010000000
VERSION     DAZFTN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(325..1266)
                     /gene="fabY"
                     /locus_tag="ACHKV2_002406"
                     /gene_synonym="yiiD"
     CDS             complement(325..1266)
                     /gene="fabY"
                     /locus_tag="ACHKV2_002406"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO6940279.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1311..1748)
                     /gene="dtd"
                     /locus_tag="ACHKV2_002407"
     CDS             complement(1311..1748)
                     /gene="dtd"
                     /locus_tag="ACHKV2_002407"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO6940280.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1745..2617)
                     /gene="yihY"
                     /locus_tag="ACHKV2_002408"
     CDS             complement(1745..2617)
                     /gene="yihY"
                     /locus_tag="ACHKV2_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO6940281.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2611..3210)
                     /gene="yihX"
                     /locus_tag="ACHKV2_002409"
     CDS             complement(2611..3210)
                     /gene="yihX"
                     /locus_tag="ACHKV2_002409"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO6940282.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3309..4094)
                     /gene="csqR"
                     /locus_tag="ACHKV2_002410"
     CDS             complement(3309..4094)
                     /gene="csqR"
                     /locus_tag="ACHKV2_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6940283.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4128..5024)
                     /gene="yihV"
                     /locus_tag="ACHKV2_002411"
     CDS             complement(4128..5024)
                     /gene="yihV"
                     /locus_tag="ACHKV2_002411"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO6940284.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5192..6088
                     /gene="yihU"
                     /locus_tag="ACHKV2_002412"
     CDS             5192..6088
                     /gene="yihU"
                     /locus_tag="ACHKV2_002412"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO6940285.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6112..6990
                     /gene="yihT"
                     /locus_tag="ACHKV2_002413"
     CDS             6112..6990
                     /gene="yihT"
                     /locus_tag="ACHKV2_002413"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO6940286.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7006..8247
                     /gene="yihS"
                     /locus_tag="ACHKV2_002414"
     CDS             7006..8247
                     /gene="yihS"
                     /locus_tag="ACHKV2_002414"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO6940287.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8314..9216
                     /gene="yihR"
                     /locus_tag="ACHKV2_002415"
     CDS             8314..9216
                     /gene="yihR"
                     /locus_tag="ACHKV2_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO6940288.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9277..10152
                     /locus_tag="ACHKV2_002416"
     CDS             9277..10152
                     /locus_tag="ACHKV2_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6940289.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10260..12296
                     /gene="yihQ"
                     /locus_tag="ACHKV2_002417"
     CDS             10260..12296
                     /gene="yihQ"
                     /locus_tag="ACHKV2_002417"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO6940290.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12342..13727
                     /gene="yihP"
                     /locus_tag="ACHKV2_002418"
     CDS             12342..13727
                     /gene="yihP"
                     /locus_tag="ACHKV2_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940291.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13770..15173
                     /gene="yihO"
                     /locus_tag="ACHKV2_002419"
     CDS             13770..15173
                     /gene="yihO"
                     /locus_tag="ACHKV2_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940292.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15240..15932
                     /gene="ompL"
                     /locus_tag="ACHKV2_002420"
     CDS             15240..15932
                     /gene="ompL"
                     /locus_tag="ACHKV2_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO6940293.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(16023..17288)
                     /gene="yihN"
                     /locus_tag="ACHKV2_002421"
     CDS             complement(16023..17288)
                     /gene="yihN"
                     /locus_tag="ACHKV2_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940294.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17390..18370)
                     /gene="yihM"
                     /locus_tag="ACHKV2_002422"
     CDS             complement(17390..18370)
                     /gene="yihM"
                     /locus_tag="ACHKV2_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO6940295.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(18378..19088)
                     /gene="yihL"
                     /locus_tag="ACHKV2_002423"
     CDS             complement(18378..19088)
                     /gene="yihL"
                     /locus_tag="ACHKV2_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6940296.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19305..21128)
                     /gene="typA"
                     /locus_tag="ACHKV2_002424"
     CDS             complement(19305..21128)
                     /gene="typA"
                     /locus_tag="ACHKV2_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO6940297.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21501..22910
                     /gene="glnA"
                     /locus_tag="ACHKV2_002425"
     CDS             21501..22910
                     /gene="glnA"
                     /locus_tag="ACHKV2_002425"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO6940298.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            23084..24133
                     /gene="glnL"
                     /locus_tag="ACHKV2_002426"
     CDS             23084..24133
                     /gene="glnL"
                     /locus_tag="ACHKV2_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO6940299.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            24145..25554
                     /gene="glnG"
                     /locus_tag="ACHKV2_002427"
     CDS             24145..25554
                     /gene="glnG"
                     /locus_tag="ACHKV2_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO6940300.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25666..25776
                     /gene="yshB"
                     /locus_tag="ACHKV2_002428"
     CDS             25666..25776
                     /gene="yshB"
                     /locus_tag="ACHKV2_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO6940301.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25966..27339)
                     /gene="hemN"
                     /locus_tag="ACHKV2_002429"
     CDS             complement(25966..27339)
                     /gene="hemN"
                     /locus_tag="ACHKV2_002429"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO6940302.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27528..28034)
                     /gene="yihI"
                     /locus_tag="ACHKV2_002430"
     CDS             complement(27528..28034)
                     /gene="yihI"
                     /locus_tag="ACHKV2_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO6940303.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(28153..28319)
                     /locus_tag="ACHKV2_002431"
                     /pseudo
     CDS             complement(28153..28319)
                     /locus_tag="ACHKV2_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28616..29248
                     /gene="yihA"
                     /locus_tag="ACHKV2_002432"
     CDS             28616..29248
                     /gene="yihA"
                     /locus_tag="ACHKV2_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO6940304.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29630..32416)
                     /gene="polA"
                     /locus_tag="ACHKV2_002433"
     CDS             complement(29630..32416)
                     /gene="polA"
                     /locus_tag="ACHKV2_002433"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO6940305.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            32780..33712
                     /gene="yihG"
                     /locus_tag="ACHKV2_002434"
     CDS             32780..33712
                     /gene="yihG"
                     /locus_tag="ACHKV2_002434"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6940306.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(33753..35183)
                     /gene="yihF"
                     /locus_tag="ACHKV2_002435"
     CDS             complement(33753..35183)
                     /gene="yihF"
                     /locus_tag="ACHKV2_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6940307.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8596 a         8985 c         8599 g         9068 t
ORIGIN      
        1 gatgccagcc cattatcttt gcataatgtt tgttagctat agatttttaa agtatttatg
       61 agaaaactat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag
      121 atgatgaatt aagcattatc tgcgataatt gctatacagc tctacaaata atatttaaat
      181 gctcctgaac agtgatgtag caagacacgt atggagccaa cgaagaataa aaatttatag
      241 agttcttatt caatataacc gaaatagcca aggactaata taaatgacct gaatgagaga
      301 ggcagcaccg cccctctcca tcagctactc ttcttcgttc ccgccctctt catacgggcc
      361 aaatggcttc gcgggcagaa cgatatacgt gccttcaaac actgcacccg gcgtctcgtc
      421 gccaaagatt tcgacctgca tctgcacccg tgcttttcgt ccgcgcgcca gacggtcgag
      481 atcgccgctt aaggcaccaa ggtcggctac cgcatggggt ttaccgctaa tcggtttgct
      541 gtagcggata tgcgcatccg ccagaataat cgttccgccg aggtggcgtt cgcgcagcat
      601 cagccagata agcccccaac cggtgagcgt cgccagtgag aataaactcc cggcaaacag
      661 cgtatggtgc ggattctgat tgccggtttc tggcatggta gtgataaatt tttgcccggt
      721 atattgctga atgcgcacgc ccattttttc actaagcggg atatgttcgt accacgcctg
      781 ttgcagctgc gcgcaccagt cgccgcgatg caaaatatca tccagagtgg cgacgggctt
      841 aatcatcaaa aaatggcgaa tcggcgtggt ggttggcgtg gtgatttctc cctgattaac
      901 aaaccccagc ttggcgaaaa actccaccgc gtcttcacgg gcgctacagg tcacgcgctt
      961 aacgccttcc tgacgcgcca ccgactccag ggtcatcgcc atcagcgtgc ctaacccttt
     1021 gtcctgcacg tcgggatgaa cggccataaa gcgaatggac gcttcattgt cggcattaat
     1081 atacagtcgg cctaccgcca ccagattacc ctgctcgtcg acgaccatct ggtgatgcgc
     1141 catcgcatcc cacgcgtcgc gttccgaacc ttttggttga tgcaggggct tacgcaacat
     1201 ttcccagcga aactgatagt aacgctctaa ttcttcttct gtttgtggaa cccgaaggtg
     1261 atacatagcg atactctctc ttgtttcccg tgacaaccct ggaagctggc tcatacctgc
     1321 aaccagaatg tcacggggcc atcattgacc agcgatacct gcatatccgc agcgaagcgt
     1381 cctgtttgcg tgttcatctc ttgctgacgg cagcgttcga cgaaatagtc atataacgcc
     1441 tctgcgcgat ccggtgatgc acctttggag aaacttgggc gcatcccccg ttcggtatct
     1501 gcggcgaggg taaactggga aaccaccagc acactgccgc ccgcctgttg cacgttgaga
     1561 ttcatcttgc cttcggcatc gctaaagatg cggtagccga gcacacgctc gcacagacgg
     1621 tttgctttct gttcgtcgtc atccttttcg acacccaata acaccaaaag tcccgcacca
     1681 atttcgcccg tcacttctcc ctccacggtg acgctggcac gggttacgcg ttgaattaat
     1741 gcaatcatgg ttcgtcgtct tcttcttgtt cagctgcttg ttttagtttg cggtattccc
     1801 cgagagtgac agtaatttcc gcgccaagca agacgataca ccatgtccag tagacccaaa
     1861 caaagagaat ggggatcacc gccagcacac cgtaaatgag ctgatatgac gggaacatgg
     1921 tgatataaag cgcgaaacct ttctttcctg cttcgaacag gagtgcagca acaaacgcgc
     1981 cgacaatcgc gtcgcggtta ggtacgcgga tggtaggaac aatgctgtac agcaaccaga
     2041 aggagatcca cgacaacagc aacggaaaaa tacgcagcac gttgtcgatg acagtattga
     2101 gatcgctcgc ccagcgcagg gagagcaaat aggaactgat cgccagactg gcccctgcca
     2161 acagcggccc cagcgttaaa atcatccagt acacggcaaa cgagtaaatt ttgggtcgcg
     2221 ctcgtttact gcgccagatg gtattcaacg cgctatcgat ggagtacatc aacaataacg
     2281 cggtgacgat cagcccgcac gccccaacgg cggtcatctt gttggaattg gcaacaaatt
     2341 gttcgatata ccgctgaata acatcgccag tagcaggcag aaagttggca aaaataaagt
     2401 gacgtaactg aatgctgacg tcggaaaaca tgggaaaagc ggcgaaaagc gcaaaaacaa
     2461 cggcaaccag cggcactaat gagagcaacg acacataggc aaggttacct gccagggttg
     2521 tcatattgtc ctcatcaatg cgttgccaga gtagttttag ccaggcccat agtggacggg
     2581 tacggtgcct ggctttgtcc tgaatggttt ttagcataac accttcgcga aatagtccgg
     2641 gatggtggtt ttatctttca ccagaatact ggtaatgccc agctgattag ctccttctat
     2701 attatcggcg ttatcgtcga aaaagaccgt atcgctgggt gaaaaacctt ccgcctgcaa
     2761 aacatgctgg taaattcgag cttcaggttt gcgcatcccc agatcttgcg acagatagat
     2821 atggtcagca gcatcacgaa tttccgggta ttcttccggc cagaaggtgg tatgcaggcg
     2881 gttagtattg gaaagcacca ccacgcgatg cccctgctca cgcagtttat gcatgatggc
     2941 gatcacttcc gggcgcagcg caacaaacac cgcctgccag ccgtgagaga actgctcgta
     3001 gcttagcggt agagccatct catgacacag cgcctctgcg aacgcttcgt cgctaatttc
     3061 cccacgctca tgctgatgaa acgcctcccc catatgaaaa ctcttcttaa gcgtagccag
     3121 cggaatacgc gttaaatcgc tccaggttcc cagcacacgg ttaaagtcga tatcgacaat
     3181 cacattacct aaatcaaaga tatagagcat ttttgcctcc tttcgcgcca caagaaatta
     3241 actgtagcgg gaaaggatgg gtttggctat gtggcaaggt atgaagccag gtcaacagtc
     3301 acaaaaatct acgcgtcttc ctggggaagg atgatttcaa tttggctgtc ttgtaagcgt
     3361 gattttagcg cagcgggcgg cagctcgtcg gtaaagagcg cggtgacctg tgccacgtta
     3421 ccaatttcaa ccgccgccga agcatgatac ttagtgtgat cggcaaccag caggatattt
     3481 ctcgcgtgcg ccatcatcgt tttcaccacg ttagcttcgt ttacatcaaa ctccatcaac
     3541 gcgccatcgc tctcaatcgc cccaacgctt gttaccagat aatcagcacg aaaatcggcc
     3601 acaaaggacg ccgctgaagg gccaatgatc ccgctattat gagagcgcaa cgtaccgccg
     3661 ggtaccatca cttcaaagcg cgggttgtgg taaagaatat gcgccacacg caggctgttg
     3721 gtgattatcc gcaaatgatt atggttaagt aacgcccggg caacatgctc aacggtcgta
     3781 ccaatggtga taaatattgt tgaaccatca ggaatatagt ctgccaccgc ttcggcaatc
     3841 gctttttttt cctcggtttg cgaaacctca cgctgctcga acgccgtatt aacgacgctg
     3901 gaagcccgac ccgcgccacc gtgatggcgc gtaatcaggc cttgctcgct taatttacga
     3961 atatcccggc gcaccgtctg cgtggagaca tccagcagat ttgccagctc atcaatattc
     4021 atatacccac gctcggcgat cagcatgagg agttggtcgt gccgcgggtt accggttagt
     4081 tcggtaaggc tcatgaaaat tcctcgaaaa accatcaccc tggcatttta tacaaaaagt
     4141 gacaaaaaag atcgggtttg atcacagtca gggatcccgg ctcgtccacc gggacgcgtg
     4201 catttcgacg ccgctacacc gctggcgaag cggactgact ccgctaaatc cccacctgtt
     4261 gccagcacca ccgccaatgc gccgtgaaaa acatcacccg cacctgtggt atctaccaca
     4321 tcaactttga aggccggttg atgttgacgc ccgccatttt ccagccagtc acagcctgcg
     4381 ctgccctggg tcacatagac atgaccattt gtgagcgttt gtgcctgttt tagcgcactg
     4441 gccatctctt tcacgcccgt taagcgcgcc agacccggtt ctgaaaaggc cgcatgatcg
     4501 cttaatgcca ccagctcgct gatatcctgc ggcgtaatat caccgtccag aacggtcatc
     4561 acgcccgcct gccgggccag agtgaaggct tttttagcgc cgtcgtgcca gcgtacatct
     4621 gccagcacaa catcccactg agagaaatca atttcctcca accactctgc gtcaggcagc
     4681 aggtccgggc tggggtagtt aatgattatc cgctcgcctt tggcatccac catgatggcg
     4741 gattgcgacg atttcgcctg tttataccgt ttggtgtagc gggtgttaac gccccaggat
     4801 tccagctctg ccagcaggct gttgccggtg tcgtcatcac cgacgcgccc aataaaatca
     4861 acctgcgctc ccagtcttgc cgccgcaacc gctgccgtcg ccgctggccc gccaccaact
     4921 tccgtataat ttctcgccac gtatttaccg ctctccgtcg gtaacccttc cacgtaatag
     4981 atgcgatcca tcacggttat acctacacaa gcaacacgaa tcatggtcat tccttaaaca
     5041 ttttgggatg cgttcatttt aatttcacca attgtttaaa aagtgacgtc tgtcaatttt
     5101 ttgactataa attacaaata cgatcaaaaa cagacaatca aagcgtgtca taaatgacaa
     5161 aaagtgacat cactgtattc aggagaaggt tatggcagca atcgcgttta tcggtttagg
     5221 acaaatgggt tcgccaatgg cgagcaattt attgcagcaa gggcaccaac ttcgcgtctt
     5281 tgatgtgaat gccgaggctg tgcggcatct ggtagacaaa ggcgcgactc ccgccgccaa
     5341 cccggcgcag gccgctaaag atgccgaatt tatcattacc atgttgccga atggcgatct
     5401 ggtgcgcagc gtgttgttcg gtgaaaacgg cgtttgcgaa agcttatcta ccgatgcgct
     5461 ggtcattgat atgtcgacca tccatccgct gcaaaccgat aaattgattg ccgatatgca
     5521 agccaaaggc ttcaacatga tggatgttcc ggtaggccgt acttctgcaa atgccattac
     5581 cggtactctg ttactgctgg ctggcggcac cgctgaacaa gttgaacgtg ccacgccgat
     5641 cctgatggcg atgggcagtg agttgatcaa cgcaggcggt ccgggcatgg ggatccgcgt
     5701 taagctcatc aacaactata tgagcatcgc gctcaatgcg ctttcggcag aagctgccgt
     5761 tttgtgcgaa gccctgaatc ttcccttcga tgttgccgtc aaagtgatga gcggtaccgc
     5821 cgccggtaaa ggccacttca ccacttcctg gccgaacaaa gtcctcagcg gggatctttc
     5881 tcccgccttc atgatcgatc ttgcccataa ggatcttggc atcgcccttg atgtcgccaa
     5941 ccagctgcat gtgccaatgc cgctgggggc cgcctcacgg gaggtttata gccaggcgcg
     6001 cgcagcgggt cgcggtcgcc aggactggtc cgccattctg gaacaggtcc gtgtcagtgc
     6061 cgggatgact gccaaagtaa aaatgtaacg actggataaa ggaataaatg aatgaataag
     6121 tacaccatca acgacattac gcgcgcatcg ggcggttttg ccatgctggc ggtcgatcag
     6181 cgcgaagcca tgcgcatgat gtttgccgcg gctggggcac ccgctccggt agccgatagc
     6241 gttttaactg atttcaaagt taacgcggcg aagaccctct cgccttatgc ctcggcgatt
     6301 ctggtagatc aacaattctg ttaccgccag gtggttgagc aaaacgcgat tgccaaaagt
     6361 tgcgccatga ttgtcgccgc cgatgagttc attcctggca acggtattcc ggtagatagc
     6421 gtggtcattg accgcaaaat caatccgcta cagatcaaac aagacggtgg caaagcctta
     6481 aaactgctgg tgctgtggcg tagtgatgaa gatgcgcagc aacgcctgga tatggtgaaa
     6541 gagtttaacg aactgtgcca ctcacacggc ctggtaagca ttattgagcc agtcgtccgc
     6601 ccaccacgtc gtggcgataa attcgatcgc gaacaagcga tcatcgatgc cgccaaagag
     6661 ctgggcgaca gtggcgctga cctctacaaa gttgaaatgc cgctttacgg taaaggtccg
     6721 caacaagagc ttctcagtgc ttcacaacgt ctgaatgacc atatcaatat gccgtgggtg
     6781 atcctctctt ccggtgtcga cgaaaaactg ttcccgcgtg ccgtacgcgt ggcaatgaca
     6841 gcgggtgcat cgggcttcct ggctggtcgt gctgtctggg catcggtcgt cggtttacca
     6901 gacaacgagc tgatgctgcg tgacgtttgc gcaccgaaat tacaacaact tggcgatatc
     6961 gtcgacgaaa tgatggctaa acgccgttaa aatggggata caaaaatgaa atggtttaac
     7021 actttaagtc acaaccgttg gctggaacag gaaaccgatc gcatctttga ttttggtaaa
     7081 aattccgtag taccgaccgg atttggctgg ttaggcaata aagggcaaat caaagaagag
     7141 atgggcaccc atctgtggat cacagctcgt atgttgcacg tttattccgt tgctgcggcg
     7201 atgggtcgac ctggcgctta ctcgttggtt gatcacggta ttaaagccat gaacggcgca
     7261 ctgcgcgata aaaaatatgg cggctggtat gcctgtgtga atgacgaggg cgtggtggat
     7321 gcctccaaac agggctatca acatttcttt gctctgctgg gtgctgccag cgccgtcaca
     7381 acgggtcacc cggaagcgcg caagctgctc gattacacca ttgaaattat cgagaaatac
     7441 ttctggagcg aagaagagca gatgtgcctg gagtcctggg acgaagcctt cagcaaaacc
     7501 gaagagtacc gcggcggcaa tgccaatatg cacgcagtgg aagctttctt aattgtttat
     7561 gacgtaacgc atgacaaaaa atggctggat cgcgcgattc gcgtggcttc cgtgattatc
     7621 cacgacgtcg caagaaataa tcattatcgc gttaacgaac acttcgatac ccagtggaat
     7681 ccgctgccgg attacaacaa agataacccg gcgcaccgct tccgcgcgtt cggaggtaca
     7741 ccaggccact ggatcgaatg gggccgttta atgctgcaca tccacgcggc cctggaagcc
     7801 cgttgcgaac aaccgccagc atggctgcta gaagatgcca aaggtctgtt taacgccacc
     7861 gtgcgtgatg cctgggcacc cgatggtgcg gacgggattg tttataccgt tgactgggaa
     7921 ggaaaaccgg tggtccgcga acgtgtgcgt tggcctatcg tcgaagcgat gggtacggcc
     7981 tacgcgctct acaccgtcac cggcgatcgc cagtacgaaa cctggtatca gacatggtgg
     8041 gactactgca tcaaatacct gatggactac gaaaatggtt catggtggca ggagctggat
     8101 gcggacaata aggtcaccac caaagtctgg gacggcaaac aggatattta tcacctgctg
     8161 cattgcctgg tgatcccgcg tatcccgtta gcgccaggcc tggctccggc agttgcggcg
     8221 ggtctgctgg atattaacgc gaaataactg actaaaaaaa cgacatatca ggctgcacgc
     8281 cgggtatgtc gttattcaat aaaggtacct cgtatgcaaa tcaccaacat gcattgcagt
     8341 ggacagaccg tttcacttgc cgcgggggat tatcacgcga ccattgtgac cgtcggtgca
     8401 ggtctggctg aattaacctt tcagggctgt catttagtta ttccacataa acccgaagag
     8461 atgccgctgg cacatctggg caaagtcctt attccgtggc cgaaccggat cgccaacggc
     8521 tgttatcgct atcaggggca ggaatatcag ttacccatta atgaacatgg ttcaaaagcc
     8581 gctattcatg ggctgctcgc ctggcgtgac tggcaaatca gcgaattaac cgccaccagc
     8641 gttaccctga cagcttttct gccgcccagt tacggctatc cgtttatgct ggcttcgcag
     8701 gttgtttatt cgctcaatgc acgcacgggt ctttccgttg agatcgcaag tcagaatatt
     8761 ggtactgtga ccgcacccta tggtgtgggt attcatcctt atttaacctg caacttaaca
     8821 tctgttgatg aatatctgtt ccagcttccg gcaaaccagg tttatgccgt cgatgagcac
     8881 gctaatccga caacgcttca ccacgttgat gagctggatt taaatttcac gcaggcgaaa
     8941 aaaatagccg cgacaaaaat cgaccacacc tttaaaaccg ctaatgatct gtgggaaata
     9001 accattaccc atccacaaca ggcgctttcg gttagccttt gttccgatca accctgggta
     9061 caaatttata gcggtgaaaa attacagcgt cagggactgg cagttgaacc tatgagttgc
     9121 ccgcctaacg cctttaattc aggtctcgac ttattattac tggaaccagg gaaaacgcac
     9181 cgcctgtttt ttaatattta cgggcaacac aattaatttt attaagaaaa gaacgagatc
     9241 attgtttaga gttgtattta cacaacagga aatattatgg acttttttac gttattttca
     9301 ccggatgggg tcatgctgga aacaaacgtg gtatgtatcg ttatcgcgtt aatgtttctt
     9361 tatacctttg tggggatatg cgcgggattt ggcggtggtt taaccactat gccgctcgtc
     9421 acgttaatgc tccccgtaaa aatggcaaca ccactctcgg taattgtcgg caccgcgacc
     9481 gccttgtacg ccacctggct gtcacgtaaa gaaacggatt ggcgatcggc aatggtactg
     9541 attattttct cgtttctggg aatacccgtc ggtttatatg cactgtcgta tttacccgac
     9601 cattttatga aagtgggctt aggcggattc cttatcctgt actcctttta cagtatgttt
     9661 attccacgcc tgccgattta tgaccgtagc tggatcgccg cacctatcgg tgcagtagca
     9721 ggcgcattag gcgcagcttt ctcaaccaac ggaccaccga tggttatcta cggtatgctg
     9781 cgtaacttag gcccggcggc atttcgtggc actctgaacg ctttcttcac cgcaaacaac
     9841 atcgcggtga tcggcgggct ggcgaccagc ggaattttga ctgtttcgat ctttaaactg
     9901 gtgctgtttt gtattcccac catgatcctc ggttcactgg ttggacaata tgtgcataaa
     9961 cgtatttcag tcaaagtgtt ccgtgtgatg gtctttttat tgctgattgc ctccggttcc
    10021 atgttgataa aaggcgcgct gggaattccc gccgctgccg cacttctacc tccgtttacc
    10081 ttgttagtta tactgcaact ggtatttggt aaaaaagtgg cggcaatccg tcaggctgaa
    10141 gcaagtaaat aatcacttat gcgggtttaa atttatatcc gcacacattc tttaattaat
    10201 taaacctgta ataacaggat ggcatgaaat attagcttta tactctatgg agattattta
    10261 tggatacgcc acgcccacaa ttaccagatt ttgaatttca tcagaataac gacagtttta
    10321 ccctacattt tcgacaacgt cttattttaa cccatagcaa agataatcct tgtttatgga
    10381 ttggctcagg tatagcggac atcgatatgt tccgcggcaa tttcagcatt aaagataaac
    10441 tacaggagaa aattgcgctt accgacgcca tcgtcagcca gtcaccggat ggttggttaa
    10501 tccatttcag tcgtggttct gacattagcg ccacgctgaa tatctctgcc gacgatcagg
    10561 gccgtttatt gctggaacta caaaacgaca accttaacca caaccgcatc tggctgcgcc
    10621 ttgccgctca accagaggac catatctacg gctgcggcga acagttttcc tacttcgatc
    10681 tgcgcggcaa accgttcccg ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa
    10741 cctatgtcac ctggcaggcc gactgcaaag aaaatgcggg cggcgactat tactggactt
    10801 tcttcccaca gcctacgttt gtcagcacgc agaagtatta ttgccatgtt gataacagtt
    10861 gctatatgaa cttcgacttt agtgccccgg aataccatga actggcgctg tgggaagaca
    10921 aagcaacgct gcgttttgaa tgtgctgaca catacatctc cctgctggaa aaattaaccg
    10981 ccctgctggg acgccagcca gaactgcccg actggattta tgacggagta acgctcggca
    11041 ttcagggcgg gacggaagtg tgccagaaga aactggacac catgcgtaac gcgggcgtga
    11101 aggtcaacgg catctgggcg caggactggt ccggtattcg tatgacctct tttggcaaac
    11161 gcgtgatgtg gaactggaag tggaacagcg aaaactaccc gcaactggat tcgcgtatca
    11221 agcagtggaa caaagagggc gtgcagttcc ttgcctatat caacccgtat gttgccagtg
    11281 ataaagatct ctgcgaagag gcggcaaagc gcggttatct ggcaaaagat gtcgccggtg
    11341 gcgactatct ggtggagttt ggcgagttct acggcggcgt tgtcgatctc accaatccgg
    11401 aagcctacgc ctggttcaag gaagttatca aaaagaacat gattgaactc ggctgcggcg
    11461 gctggatggc tgacttcggc gagtatctgc ccaccgacac gtacctgcat aacggcatca
    11521 gcgcggaaat tatgcataac gcctggcccg cgctgtgggc gaaatgtaac tacgaagccc
    11581 ttgaagaaac gggcaagctc ggcgagatcc tcttctttat gcgcgccggt tctaccggta
    11641 gccagaaata ctccaccatg atgtgggcgg gtgaccagaa cgtggactgg agtctggacg
    11701 atggcctggc gtcggttgtc ccggcggcgc tatctctggc aatgaccgga catggcctgc
    11761 atcacagcga cattggcggt tataccaccc tgtttgagat gaagcgcagc aaagagctgc
    11821 tgctgcgctg gtgcgatttc agcgccttca cgccgatgat gcgcacccac gaaggtaacc
    11881 gtcctggcga caactggcag tttgacggcg acgcggaaac catcgcccat ttcgcccgta
    11941 tgaccaccgt cttcaccacc ctgaaacctt acctgaaaga ggccgtcgcg ctgaatgcga
    12001 agtccggcct gccggttatg cgcccgctgt tcctgcatta cgaagacgac gcgcagacct
    12061 attccctgaa atatcagtac ctgttaggtc gcgacattct ggttgctccg gtacacgaag
    12121 aaggtcgcag cgactggacg ctctatctgc cagaggataa ctgggtccac gcctggaccg
    12181 gagaaacctt ccacggtgga gaaataactg ttgaagcgcc catcggcaag ccgccggtct
    12241 tttatcgcgc cgacagcgaa tgggctgcac tgttcgcctc gttaaaaaac atctaatcat
    12301 gcccgcccgt ggggaaactc acgggcaaat ggagaataac gatgagtcac atcacaacgg
    12361 aagatccggc aactttgcgc ctgcccttta aagagaaact ctcttacggt atcggcgatc
    12421 tggcctctaa catcctgctg gatattggta cgctttatct tttgaagttt tataccgacg
    12481 ttctggggct acctggcacc tatggcggca ttatcttttt gatctcgaaa ttctttaccg
    12541 cctttaccga tatgggaacc ggcatcatgt tggattcccg gcgtaagatt ggcccgaaag
    12601 gtaagttccg ccctttcatt ttgtacgcgt cattcccggt caccttattg gcaatcgcta
    12661 actttatcgg cacaccgttc gatgtcactg gtaaaacggt gatggccact attctgttta
    12721 tgctctacgg gctgtttttc agcatgatga actgctccta tggcgcgatg gtgcctgcta
    12781 ttaccaaaaa ccccaacgag cgcgcatcac tggcggcatg gcgtcaggga ggcgctacgc
    12841 tgggcctgct gctgtgcacg gtgggattcg tgccggttat gaatcttatc gaaggtaatc
    12901 agcaacttgg ctatatcttc gccgccacgc tgttttcact gttcggcctg ctgtttatgt
    12961 ggatctgcta ctcgggcgtg aaagagcgtt atgtcgaaac ccaaccagcc aatccggcgc
    13021 aaaagcctgg cctgctgcaa tctttccgcg caattgccgg taaccgccca ctgttcattc
    13081 tgtgcattgc caacctctgc actttagggg cgtttaacgt caagctcgcc atccaggtct
    13141 attacaccca gtacgtactt aacgatccca tcctgttgtc atatatggga tttttcagca
    13201 tgggctgtat tttcatcggt gtgttcctga tgcctggcgc agtcaggcgt tttggtaaga
    13261 agaaggtcta tatcggcggc ctactgattt gggtgctggg cgatctgctc aactatttct
    13321 tcagcggcgg ttcggtcagc ttcgtggcgt tctcctgcct ggcattcttc ggctcagcgt
    13381 ttgttaacag cctgaactgg gcgctggttt ccgacaccgt cgagtacggc gagtggcgta
    13441 ccggcgttcg ttcggaagga acggtctaca ccggcttcac cttctttcgc aaagtgtctc
    13501 aggcgctggc tggtttcttc cccggctgga tgctgacgca aatcggttat gtgccgaacg
    13561 tggcgcaggc tgaccacacc attgaagggt tgcgccagct gatcttcatc tacccaagcg
    13621 cactggcggt agtcaccatt gtggcgatgg gctgcttcta cagcctgaac gagaagatgt
    13681 atgtccgcat tgttgaagaa atagaagccc gtaaacgcac ggcgtaataa taataaacaa
    13741 tgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg acattaaaac
    13801 tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt tggttcgaat ttaatgctta
    13861 gcatcggtac tctgtatctc ctcaaatttt ataccgatga attagggatg ccagcttact
    13921 atggcgggat tatctttctg gtggcgaagt tttttaccgc gtttaccgat atgctcaccg
    13981 gatttttact cgattcgcgg aaaaatattg ggcctaaagg caaatttcgt ccctttattt
    14041 tatacgcagc ggttccggcg gcgttaattg caacgctcca gtttatcgcc accactttta
    14101 gtttgccggt taaaacgacg attgccaccg cgctgtttat gatgtttgga ctttcataca
    14161 gtctgatgaa ctgctcgtat ggtgcgatga tcccggccat tactaaaaac ccgaatgagc
    14221 gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg atttgtaccg
    14281 tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg
    14341 cactgatgtt ctccattggt ggctttattt ttatgatgct gtgctacaga ggcgtcaaag
    14401 agcattatgt ggacacagca ccaaccggac ataaagccag tattctcaaa tctttttgcg
    14461 cgatatttcg gaatccgcca ttgctggttt tatgcattgc taacttgtgt accctggcgg
    14521 catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg aatgatatta
    14581 atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc gtattactgg
    14641 tgccattaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc atggtgctat
    14701 gggcggttgg tgatatcctg aattatttct ggggaagtaa ctctttcact ttcgtcatgt
    14761 tctcttgtat cgcctttttt ggcacggcct ttgtaaacag cctgaactgg gcactggtgc
    14821 cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt tctgtttata
    14881 ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg ccaggcatta
    14941 tgctgacgca aatcggttat gttcccaaca tcgcgcaaag tgatgcgaca ttacagggtt
    15001 tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca ttgacgatgg
    15061 gattctttta cacactcaat gaaaaacggt ttgcgttgat tattgaggaa atcaaccaac
    15121 gcaaaaataa agagatggcg acagaagaaa aaacggcttc cgtaacgtta taaatgtcaa
    15181 tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaat ggacttatta
    15241 tgaaaaatat taatgcaata attttattat cttctctgac ctctgcctca gtattcgctg
    15301 gcgcatacgt cgaaaacagg gaggcatata atctcgcctc cgatcagggc gaggttatgc
    15361 tacgtgtggg ttataacttc gatatgggcg cgggtattat gttaaccaat acctacacct
    15421 ttcagcgaga agatgaacta aaacatggat ataacgaaat cgaaggttgg tatccgttat
    15481 ttaaaccaac cgataaatta accatccagc ccggtggctt aattaatgat aagagtatcg
    15541 gttcgggtgg tgctgtttat ctggatgtta actacaaatt tactccgtgg tttaacctga
    15601 cagtgcgcaa ccgttttaac cataataact acagctcaac cgatttaaac ggtgatctgg
    15661 ataataatga cagctacgag attggcaact actggaactt tattattacc gataagttct
    15721 catatacctt tgagccgcat tatttttata acattaatga tttcaatagc ggcaatggca
    15781 cgaagcatca ctgggaaatc actaacacct tccgttaccg cattaacgag cactggctgc
    15841 cctatattga attgcgttgg ttagatcgca acgtggaacc gtaccaccgc gagcaaaacc
    15901 agattcgtat cgggacgaag tatttcttct gatgtgaaac tgccggatgc ggcgtaaacg
    15961 ccttatccgg cctacggttg atacggtttt cgttggccgg attaagtaca tccggccatt
    16021 aattaagctt cagccgccag catctctttc ttacgttggc taatacgctt aaccagcatt
    16081 acggaaacca ccgcaccaca gaatgcgaag caggccataa tgccaaacac gattttgtag
    16141 ccaataatcc ccggatttaa atccagaatg tagccataca gactgaagca gaacatcgcc
    16201 ggggcgtaac caataaagct acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt
    16261 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
    16321 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
    16381 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
    16441 aggattttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
    16501 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
    16561 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
    16621 agccagatag ttttgtcttt cagcaccgag gtcatactgg tgttttgcga tgccccgtct
    16681 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaatagta
    16741 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
    16801 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
    16861 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
    16921 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
    16981 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
    17041 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
    17101 gacagaaaaa agcccactgt ggtaacaaat gagtttaccg acatagcatt accaatttga
    17161 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
    17221 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
    17281 gtgagcataa catcccctaa gcgattttaa aaatcactgt tttaaaggca ataagatacc
    17341 gaccattctt atggaatggt tttattaact gcgtatgagt taaaacttgt tagtggttca
    17401 gaagatggct cgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
    17461 taattgcatc ttctttttct ttacgtaacc gctcatccag caaatgcttt tctggcaatg
    17521 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
    17581 acgctggagc ataataatca acctcttctt ccaggccata tgccgtcacc tgcggttctt
    17641 catcgccgag gcagataagg tgtgttaata actctttgaa cggcatatct ccctgacctg
    17701 aaatacaggc tttatgaccc aggccacctt gttctttaac gatcaaggca tctttgatat
    17761 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc tatgggatgc tcatttgcat
    17821 tgatcatatt cgcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
    17881 gcgacaccaa ctcatgactt tttaaatctt catgctgttc gatcgtaaaa gtcaggccgc
    17941 tatcctgata cgcttcccgc acataggcaa tgtcgttagc gatgatcgat aatacgtcgc
    18001 gcaaattacc ttcataacgt ggataaaaac gtacggacga tgccccagtt ttcaacgcga
    18061 tggcgacggc ttcgtcaata gatgccttat ctgaggcgct ggtttcaata tgaatatcaa
    18121 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
    18181 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
    18241 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
    18301 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
    18361 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
    18421 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
    18481 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
    18541 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
    18601 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
    18661 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
    18721 gttgatggtg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
    18781 ttctcggtaa acccccagga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
    18841 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
    18901 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
    18961 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
    19021 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
    19081 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
    19141 aaagaaagca attaacggcg tatgactttg cagataattg tgacaaagct ctctattgac
    19201 gtaaccacag acttataagg gagatactcg cattggtgtg cgctcacgac aggcaaaaaa
    19261 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
    19321 gcgcggttgg cgcgacgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
    19381 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
    19441 atgcggatag gcggaaccag aacaacggct tcgtcagtac cggaagcacg catgttggtc
    19501 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
    19561 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaaa
    19621 ccgaacagcg cgaacgcgac cgctttaccc tgaccgttag agatcagtac gccgttctga
    19681 cgctgaccca cttcacccgg acgcacgtcg tcgtagtggc tgaaggtgga gtacagcaga
    19741 cccgtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
    19801 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
    19861 ttacgttcgc ccagcgcctg cattacagaa ccctgatgct gttcttcaac gtccagagtc
    19921 acgttttcat acggctcttg tttacgaccg tcgatttcac ggaagataac tttcggacgg
    19981 gataccgcca gttcgaaacc ttcacgacgc atgttttcga tcagaacaga caggtgcagt
    20041 tcgccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
    20101 acgttgtgta ccagttcttt gttcagacga tccaggatct gacgagacgt tacgaactta
    20161 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
    20221 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
    20281 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
    20341 atacgttcca gaccgaggtg gcccagcact ttaccaactt tcgcgttgcg ggttttgcct
    20401 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccgcgctt gatgcggcca
    20461 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
    20521 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctggta cagcggggtc
    20581 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
    20641 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
    20701 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
    20761 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
    20821 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
    20881 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
    20941 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
    21001 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
    21061 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
    21121 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
    21181 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
    21241 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
    21301 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
    21361 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacagt tttcgcttta
    21421 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
    21481 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
    21541 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacag cacgtcacta
    21601 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
    21661 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
    21721 ccgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
    21781 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
    21841 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
    21901 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
    21961 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
    22021 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
    22081 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
    22141 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
    22201 acgaaattca gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg
    22261 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
    22321 cgctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
    22381 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
    22441 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagctccg gtaatgctgg
    22501 cttactctgc acgcaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
    22561 cacgtcgtat cgaagtacgt ttcccggatc cggcggctaa cccgtacctg tgctttgctg
    22621 ccctgctgat ggccggtctt gatggcatca agaacaagat ccatccgggc gaagccatgg
    22681 acaaaaacct gtatgacctg ccgccggaag aagcgaaaga gatcccacag gttgcaggct
    22741 ctctggaaga agcactgaac gagctggatc tggaccgcga gttcctgaaa gccggtggcg
    22801 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
    22861 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
    22921 tgtactaccc ggcgcaacaa cgccgggatt tagttgccgt ggaaactttc agcccatctc
    22981 tgcatgggct tttttctccg tcaattctct gatgcttcgc gctttttatc cgtaaaaagc
    23041 tataatgcac taaaatggtg caacctgttc aggagactgc tttatggcaa caggcacgca
    23101 gcccgatgct gggcagatcc tcaactcgct gattaacagt attttgttaa tcgatgacaa
    23161 cctggcgatc cattacgcca accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa
    23221 attgtttggt acgccgttac cggaactgtt gagctacttc tcattaaata ttgagctgat
    23281 gcaagaaagt ctggaggcgg ggcaaggttt taccgataac gaagtgacgc tggtcatcga
    23341 cggtcgctcg catatccttt ctgtgacggc ccagcgtatg ccggacggca tgatcctgct
    23401 ggagatggcg ccgatggata accagcgccg cttaagccag gaacagctac aacatgctca
    23461 acaagttgct gcccgtgatt tagtgcgtgg cctggcacat gagattaaaa atccgcttgg
    23521 cggtttacgt ggcgcggcgc agctactcag caaagcatta cctgacccgt cactgctgga
    23581 atataccaaa gtgattatcg aacaggcgga ccggctgcga aatctggtcg accgtctgtt
    23641 ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt cacaaagtag ctgaacgcgt
    23701 ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg attacgatcc
    23761 cagcctgccg gaactggcgc acgacccgga tcaaattgaa caggtcttgc tgaatattgt
    23821 gcgcaatgcg ctacaggcgc tggggccgga aggcggtgaa atcattctgc gtacccgcac
    23881 cgcgtttcaa ctgaccttac acggcgaacg ctatcggctg gcggcgcgga ttgatgtgga
    23941 agataacggg ccaggcattc cgcctcattt gcaggatacg ctgttttacc cgatggtcag
    24001 cggccgcgaa ggtggcaccg ggcttggctt atccatcgct cgtaatttga ttgatcagca
    24061 ttcaggcaaa attgaattta ccagttggcc agggcatacc gagttctcgg tttacctgcc
    24121 tatcaggaaa taaaggtgac gtttatgcaa cgagggatag tctgggtagt cgatgacgat
    24181 agttccatcc gttgggtgct tgaacgtgcg ctcgctggag cgggtttaac ctgtacaaca
    24241 tttgagaacg gcgcggaggt actggaggcg ctggcgagca aaacgccgga tgtgctgctt
    24301 tcagatatcc gtatgccggg aatggacggg ctggcactgc tcaagcagat taaacagcgc
    24361 catccgatgc ttccggtcat cattatgacc gcacattccg atctggatgc tgccgtcagc
    24421 gcctatcaac aaggggcgtt tgattatctg cccaaaccgt ttgatatcga cgaagccgtg
    24481 gcgctggttg agcgcgccat cagtcattac caggaacagc agcagccgcg taatgttcag
    24541 cttaacggcc caacgaccga tatcatcggc gaagcgccag ccatgcagga cgtgttccgt
    24601 attatcggtc ggctttcgcg ttcttctatt agcgtgctga ttaacggcga atccggcacc
    24661 ggtaaagaac tggtcgctca tgccctgcat cgccacagtc cgcgagccaa agcgccattt
    24721 atcgctctga atatggcggc tatcccgaag gatttgatcg aatcagaact gtttggtcac
    24781 gagaaaggcg cgtttaccgg cgcgaatacc attcgtcagg ggcgttttga acaggctgat
    24841 ggcggtacat tatttctcga tgaaattggc gatatgccgc tggatgtgca gacgcgtttg
    24901 ctgcgcgtgc tggcagacgg tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg
    24961 gatgtgcgga ttatcgctgc cactcaccag aatctcgaac agcgggtgca ggaaggtaag
    25021 ttccgtgagg atctgttcca ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgt
    25081 gagcgtcggg aagatattcc ccgcctggcg cgccattttt tacaggttgc cgcgcgagaa
    25141 ctgggcgtag aagcgaagtt gctgcatccg gaaaccgaag ccgcgttgac gcgtctggcg
    25201 tggccaggca acgtgcgcca gctggaaaac acctgccgct ggctaacggt aatggctgcc
    25261 ggacaggaag tgttgattca ggatttgcct ggcgaactgt ttgaatcaac ggttgcggag
    25321 agtacttcgc aaatgcaacc ggacagttgg gcgacgcttc ttgcgcagtg ggcagacaga
    25381 gcgctacgtt ccggtcatca aaatctgctt tccgaagcgc agccagagct ggagcggacg
    25441 ttactgacga ccgcgttgcg acatacgcag gggcataaac aggaggcggc gcggctactc
    25501 ggctggggcc gcaacaccct gacgcgtaag ttaaaagagc tggggatgga gtgattcacg
    25561 gcttgtgtgt aaagattgat tattgagcgc aaattgctgg tattttgcgc tttactgttc
    25621 cgataagttc agtatgatct tgcccggaaa acggggagag tcattatgct ggaatcaata
    25681 attaatctgg tatcgagtgg cgcagttgac agccacacac cgcaaactgc tgttgctgcc
    25741 gtgctgtgtg ccgcgatgat tgggctgttt agttgagttt cagattgctg atgaagtgct
    25801 ctttgttcat gccagatgct gcgtgaacgc cttatccggc ctacaaaagc aatattttca
    25861 acaggttttg taggcctgat aagcgtagcg catcaggcaa tcttgttctc agcacattga
    25921 tggcgctttc gttttctact ttgtaaacga agcgccattc actatttaaa tcacccgaga
    25981 gaactgctgc atccgcgctt tctggcgcag ataggtatca aagcacatgc aaatgttgcg
    26041 gatcagcaag cgacctttcg ccgtcacctg tattcccttc tcatccacat ccaccagccc
    26101 atcttttgct aacggggcga gcagcttgag atcttccgca aagtaatcag cgaagtgcaa
    26161 atcccactgt ttctcaatag gggcgtaatc cagacggaag ttgcagatga gcgacttaat
    26221 cacatcgcgg cgaatacagt catcacgcgt tagcgcaata ccacgccaca gcgcattgcc
    26281 ttgttcatcc acttgctgat agtactgctt caactctttc tggttctgcg cgtagcagtc
    26341 gccaatcatg ctgatggcgg aaacgcccat ccccagcaga tcggtatcgc cctgagtggt
    26401 gtagccctgg aaattacgat gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc
    26461 cggacgggca aagtgatcca taccgataaa ctgatagccc gattgcgtca ggaaggcgat
    26521 ggtttcctgc aggatatcga gtttttgctg cggactcggc aggtcagcat ctttgatttt
    26581 gcgctgagca gcaaaaatgg tcggcagatg cgcatagtta aagacgctca gacggtcagg
    26641 gtttagctcc gccacgcgtt tcagggtaaa ggcgaaactt tccggcgtct gtttcggcag
    26701 gccgtaaatc aggtcgatgt tggtggaggt aaagccaatc tcacgcgcat ggttaagcag
    26761 cgcaaagata aactcttcat cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa
    26821 gtcctgcacg cccatactca ggcgattaaa gccctcggcg cgtaaatgat cgagtacatc
    26881 cagttcgatt tcccgcggat cgacttcgat cgaaatctcc gcatcggcat tgaactggaa
    26941 gttttcgcgc agcagcttca tcaggcggct gatttgcgct ttattaagat acgtcggcgt
    27001 tccgccgccc cagtgcagct ggctgacgtg acgcccggcg aacagcggtg cacgatggac
    27061 gatttcttgc tccagcgcgt ccagatactg atcggccttg tgctgctggc gagtaacaat
    27121 cttattgcag ccgcagaagt aacaaagctt atggcagaac gggatatgta cgtagagaga
    27181 taatggacgc tcaggatagc gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc
    27241 tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc ccggaatagt tatatttctg
    27301 gatcagggcc agatcccagt cgatttgctg tacagacatg ctcactcctt ccgatggcgt
    27361 ctctggcggc tacggcgtac cggcggatgc ccgtttcggg tcatcagccg gttgcgcagc
    27421 cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata accacaccag ataacatata
    27481 accggaaggg ttatcagcag aacgggaagg cccacggcgg caatccgtta gttgccccgc
    27541 agcagacgca tcatgtcttc ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa
    27601 gagaggccga gtttctgcat caactcatca atacgatcca gtttagcatc cacccaggat
    27661 tgctcttcgg cactcagggt ttcgcctgct tccagacgtt ccagcagcgc atccagacgc
    27721 tcatccgttt ctagtaattc caactccgcc tgcggtgaaa gcataggttt ctcactcttc
    27781 ggtttgtgct gtttggtgac tttttcagtc acgcccaatg gaataggggt tttactgcca
    27841 atacgtggat ctttcggtgc gttctggcct ttgctgcctg acgtgttgtt tccgcccgct
    27901 gcgcggctgc ccggcgcatg accacgacgt tttttctgac gcttgcggtc acgagcttcc
    27961 tgattcagct cttcgcgcgt tttgcgacgc gcttttgcat gacctttgct gcgtgatggt
    28021 gaagatggtt tcataatggt tcgtctttta gccgttttat tcagtataga tttgcggcgg
    28081 aatctaacag aaagcaagca aagaaaaaag gcgacagatt actctgtcgc cttttttcct
    28141 gactcataac ccttaacggg tcttacaatc cttgcaggcc gccaacacac cctgtttttc
    28201 cattagccgg aacaatccgt gttgattcca tttccgttta attacgtctc cggacgtttg
    28261 tcttcctgac aatcctctgt cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt
    28321 gttcctgtta gcgtcctcgc tctatgggtg ctactttacg cctttgcttt aaacaaacaa
    28381 tcaaccgcca tcagtatttg cacattttca gagttttata taaaagcaat taactgattt
    28441 atatagctta tttgacatgc atcgcagaat tttctctgct atttcgcata aagactatcg
    28501 gcaatgtctc acaaagcgta tggcttttac ttacaacgct cacgccgaaa aatccgcctt
    28561 ttattatggt tcaggtataa tcgccgcaaa gctttgactg acggagacga ccgctttgac
    28621 taatttgaat tatcaacaga cgcattttgt gatgagtgcg cctgatattc gccacctacc
    28681 ttccgatacc ggaattgaag tggcttttgc aggccgttcc aacgcaggta aatccagcgc
    28741 gctgaacacg ctgactaacc agaaaagcct ggctcgtacc tcaaaaaccc ctggacgcac
    28801 ccagcttatc aacctgtttg aagtggctga cggcaagcgt ctggttgact tgcctgggta
    28861 cggttatgcg gaagtcccgg aagagatgaa gcgcaaatgg cagcgcgcgc tcggcgagta
    28921 cctcgaaaaa cgtcagagcc tacaaggtct ggtagtgcta atggatattc gccatccgct
    28981 gaaagatttg gatcagcaga tgattgagtg ggcggtagac agcaatatcg ccgtgctggt
    29041 actgctgacc aaagcggaca aactggcaag cggcgcacgt aaagcgcaat tgaatatggt
    29101 gcgtgaagcg gtgctggcgt ttaacggtga tgtgcaggtt gaaacgtttt cttcgctgaa
    29161 gaaacaaggc gtggacaagc tgcggcagaa actggatacc tggtttaacg agatgcagcc
    29221 tgtagaagaa acgcaggacg gcgaataatt ttcttacctt aatgcttatg ccggatgtgg
    29281 cgtatccggc ccataaattc aatagattac aattctaccg ttggcctgat aaacatggtg
    29341 catcaggcat tacggatctt tcctttcgcc caataaaaaa cgccccagtc attactgact
    29401 ggggcggcta aaatattcag ccaaatccga ttacgtgaag taaaaggtct gaaagataga
    29461 acatcttacc tctgtaccct acgcgactaa ctttactctt ttttgttcag ttcagaaagc
    29521 actttttgta attttttttc agttgttgca taggaatttc taatagccat cacaaaacgt
    29581 gacagcttat gttgcttact tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct
    29641 gatcccagtt ttcgccactc cccacttcca ccagcaacgg cacatccaga cgggtacagt
    29701 tttccatcag ttgatgaatc tgcttcgcaa cggcatcgac atcatctcta tgaacttcaa
    29761 ataccagttc atcgtgtacc tgcatgatca tacgtacacg cggttgctca gcctgtaacc
    29821 acgcatcaac ggcaatcatc gcccgtttga taatgtcggc ggcggttccc tgcattggcg
    29881 cgttaatggc tgcacgttca gccgctgcac gacgagcacc attgctggat ttgatatccg
    29941 gcagatacag acggcgtccg tccagcgttt caacgtagcc ctgctctttc gcctgagcac
    30001 gggtgcgttc catatactcc agcacgccag ggtagcgttc gaagtaaagg tccatgtact
    30061 tctgcgcttc tttacgtgga atgttcaatt gccgcgccag accgaaagca ctcatgccat
    30121 aaatcagacc aaagttgatc gctttcgcgc tacggcgttg ctcgctggtg acggtttcca
    30181 gtggcaaacc aaacacttct gccgccgttg cccggtggat atcttttcct tccgcgaatg
    30241 cggtcagcaa gcctttgtca cgcgagagat gcgccataat gcgcagttca atctgcgagt
    30301 agtccgctga gacaatcaca taatcctctg gcgcaataaa cgcctggcgg atacgacgac
    30361 cttcttcgtt acgcaccgga atgttttgca ggttaggatc ggttgacgat aaacgtcccg
    30421 ttgcagttac agcctggtga taagaggtat gcacacgccc ggttttcggg ttgatcatca
    30481 gcggcagctt gtcggtgtaa gtcgatttca gcttcgccag accacgatac tccagaatca
    30541 cttttggcaa cggatagtcc agcgccagtt cttccagtac ctcttccgac gttgacggcg
    30601 cgccacccgg cgttttcttc agcggtttaa tgccctgttt ttcaaacaga atggtttgta
    30661 actgcttggt ggaagaaagg ttaaattcct cacctgcaat ttcatgcgct ttcttttcca
    30721 gctcagccag acgaagggtg agctcttcag aatgattgtg cagcactttc ggatcgatct
    30781 tcacaccgtt acgttcaatg cgtgaaagta ctggcaccag cggcatttcg atattctcga
    30841 agacgttcaa cggccctttg tgtttttgca gatccggcca cattttcaga tgcaactgca
    30901 aggtgacatc agcatcttcg gcggcgtaac gtccggcctc ttcgagggca atctggttaa
    30961 aagtcagttg atttttgcct ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca
    31021 accaacgttc cgcgaggctg tccatatcgt gacgcccggc aacgctattg agaatgtagg
    31081 actccagcat ggtatcaaac gcaatcccac gcagctcaat gccgtaattc gccagaatac
    31141 cgcgatcgta tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt
    31201 ttagcaactc gagtgcacgc tcgcgagaga tttgatcggg cgcatcaaga taatcatgag
    31261 caaccggaat atatgccgct acgcctggct cgatagcaaa agaaagcccg accaggttag
    31321 ctgagatgtt atcaaggctg tcggtttcgg tatcaaatgc aaataccggc gctttttcca
    31381 gcttcgcaat ccacgctttc agtgtttctt catcaaggat ggtgacgtag ttgtcataag
    31441 aaatcaccgt tgccgtcact tctggtgctt cgtctgcaac actggtttcc tgtggcttcg
    31501 cggctggttt tgcccctttg gcctgtaacc atttgcccgc ttcgacatca gcagtccagc
    31561 gtttgaactc atactttttg aacagcccca acaactcttc cgctgccggt tgctgcactt
    31621 ccagttgttc acaggtcagc tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg
    31681 agagataagc aacttctttg ttttgctcga gcttcgctgc cattgttttc gcgccacgga
    31741 agctcaaccc agcaattttt tctggctcgg catacagcgt atccagcccg ccaagacctt
    31801 gcagcaatgc ctgcgcggtt ttttcaccga cgcccggtac gccaggaatg ttatcagagg
    31861 agtcacccat cagcgccagg aaatcgatga tcagttctgg cggcacgccg tacttattca
    31921 ccacctcttc cggtccgagg atggtattcg tcatggtgtt gataagcgta atatttggcg
    31981 tcaccagctg cgccatatct ttatcgccag tgctgatcag caccggacgc ccggcttttt
    32041 cggcttcgcg cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca
    32101 gcggcagtcc catcgcttta accatcgcgt gcaagggttc gatttgtgct cgcagatcgt
    32161 ccggcattgg cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc
    32221 ccttggcgtc aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca
    32281 gcatgttgag gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg
    32341 gaaacgcgtg atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt
    32401 gggggatctg aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca
    32461 gacaggataa aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg
    32521 gatcgctcgc aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa
    32581 tttattatca tgctttttac agcatgatgt ttgtctttgt ttttcatgca gttaaaaata
    32641 aatccatccc taataattga gataatggga ttttttctaa tgtggataga tatgaattat
    32701 ttttctcctt aaggatcatc cgttatttgg gtcgttttct gctaaaatct gcgccttgcg
    32761 cttctttagc gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact
    32821 cgctgcgata acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc
    32881 cgtcccgatc atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg
    32941 aaaggtttcg cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt
    33001 actgcatctg aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa
    33061 gaactggtat ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt
    33121 gctgtttcgt aagcacattc cgatgaataa atatttcctc aagcaacagc tggcctgggt
    33181 gccatttctt ggcctggcgt gctgggcgct ggatatgccg tttatgaagc gttattcccg
    33241 cgcttattta ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc
    33301 ttgtgaaaag tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt
    33361 cacgcaagaa aaacatcagc aaacccactc cactttccaa aacttgttgc caccaaaggc
    33421 ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt
    33481 tacgttgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact
    33541 gacgcggatt gtcgtacatg tggatttaca gcctattgcc gatgagttac atggcgacta
    33601 catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcat tgtggcagga
    33661 aaaagaccga cttctgacgt cgttgatgtc atcacagcgg caggacaagt aaattttgct
    33721 cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac
    33781 ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg tgtataacgc
    33841 agctgcagcg aggcggtatt gttgtcaatc gtgattaact gaaacatctg acctaatgtc
    33901 atcatcccgc tgatttgttt atcagcctgc ttttgcgctt tttccgcatc cattccttca
    33961 gataaattaa gctgttttgc ggtttctgtg acgacattaa gcggtaactt cacatcaaaa
    34021 ttgagcgatt tgatatcttt gtttgtggat gatgaagatt tggctgggtc ggcaatactg
    34081 atatccagat tggcatttag ttcgccgagt gcgttcttcc atcttaccgg ttgtttaatg
    34141 gtcggctcac tttgttgtaa taacggtaaa tactctttga acaatgcagc attcacttct
    34201 tgcagagcaa cttcatcgtt tgctaattca gggtgtgcag caagctgctt ctgcatcgca
    34261 atgttatact ggatgataaa ttggcgcacc gctgagggat caatagattc agcaatcaat
    34321 gaccactcac cactaccgag ttgctgattc ccttttgtta acttatcaag ttcgtaggtc
    34381 agattgacat tgatataatc tttgtcttta acgcgatcca gcctggtttt tgcaacgaaa
    34441 gcatcgattt gggcaacgtc ttccccgtga ttgataatgt taatctgatt aaatttcgac
    34501 tcgctttcac caaccggaaa tttcttttct tctaatcgcg ccagagaatc aatggtaaaa
    34561 ctttttgccg ttactttgtt cattggtgat aattgcagaa caatatcttc gactttgcct
    34621 tcaatatcca ggttagacaa tgaactacta tcaccattaa aagtaaactg gccctcacca
    34681 aaagaaaact ttccatactc tgcaggggca acatctaatg tcgtagagaa tgagccacca
    34741 aacgcgaatc gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat aaatagcggt
    34801 tgtgtcagtt cattattcac taagttcact tttgccgctg ccagtgctgg gatgacatta
    34861 ccatgcatta acattgtgat gggcaacgga ccgtgctcaa catccacatc aaatacaact
    34921 ttctggcctg gcttgatatt gagatcgggt gcaccgttat cgaatgtcat ttgcatttga
    34981 aaacgggaat taaaaaagcc tttgtcaaaa ttttgataac ttaacgtcat atcgtaggca
    35041 tgttcacctt tctttttagc atcgttaaaa tctttaataa atttttcgac accaggctga
    35101 atttgcgtac ctgtgtacca tgtgccacca ccccagatta cggctaacgc aacaataaca
    35161 cctgtagctg acttacgtat catataaata ttccctgata aaacagcaaa actttgaata
    35221 tccacttatg ctgaaataat gtccagcg
//
LOCUS       DAZFTN010000047        35032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFTN010000047 DAZFTN010000000
VERSION     DAZFTN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..764
                     /locus_tag="ACHKV2_002436"
     CDS             168..764
                     /locus_tag="ACHKV2_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940308.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            851..1576
                     /locus_tag="ACHKV2_002437"
     CDS             851..1576
                     /locus_tag="ACHKV2_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6940309.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1618..4218
                     /locus_tag="ACHKV2_002438"
     CDS             1618..4218
                     /locus_tag="ACHKV2_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO6940310.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4232..4780
                     /locus_tag="ACHKV2_002439"
     CDS             4232..4780
                     /locus_tag="ACHKV2_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940311.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4795..5400
                     /locus_tag="ACHKV2_002440"
     CDS             4795..5400
                     /locus_tag="ACHKV2_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940312.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5421..6011
                     /locus_tag="ACHKV2_002441"
     CDS             5421..6011
                     /locus_tag="ACHKV2_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6940313.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            6023..7132
                     /locus_tag="ACHKV2_002442"
     CDS             6023..7132
                     /locus_tag="ACHKV2_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6940314.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7267..8061
                     /gene="panB"
                     /locus_tag="ACHKV2_002443"
     CDS             7267..8061
                     /gene="panB"
                     /locus_tag="ACHKV2_002443"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO6940315.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8073..8924
                     /gene="panC"
                     /locus_tag="ACHKV2_002444"
     CDS             8073..8924
                     /gene="panC"
                     /locus_tag="ACHKV2_002444"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO6940316.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9223..10119)
                     /gene="rpnC"
                     /locus_tag="ACHKV2_002445"
     CDS             complement(9223..10119)
                     /gene="rpnC"
                     /locus_tag="ACHKV2_002445"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO6940317.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10393..10773
                     /gene="panD"
                     /locus_tag="ACHKV2_002446"
     CDS             10393..10773
                     /gene="panD"
                     /locus_tag="ACHKV2_002446"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO6940318.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10777..12006)
                     /gene="yadE"
                     /locus_tag="ACHKV2_002447"
     CDS             complement(10777..12006)
                     /gene="yadE"
                     /locus_tag="ACHKV2_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6940319.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12070..12510)
                     /gene="yadI"
                     /locus_tag="ACHKV2_002448"
     CDS             complement(12070..12510)
                     /gene="yadI"
                     /locus_tag="ACHKV2_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6940320.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12615..13385)
                     /gene="yadH"
                     /locus_tag="ACHKV2_002449"
     CDS             complement(12615..13385)
                     /gene="yadH"
                     /locus_tag="ACHKV2_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6940321.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13382..14308)
                     /gene="yadG"
                     /locus_tag="ACHKV2_002450"
     CDS             complement(13382..14308)
                     /gene="yadG"
                     /locus_tag="ACHKV2_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6940322.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14417..15079
                     /gene="can"
                     /locus_tag="ACHKV2_002451"
     CDS             14417..15079
                     /gene="can"
                     /locus_tag="ACHKV2_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO6940323.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15120..15656)
                     /gene="hpt"
                     /locus_tag="ACHKV2_002452"
     CDS             complement(15120..15656)
                     /gene="hpt"
                     /locus_tag="ACHKV2_002452"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO6940324.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15862..18252
                     /gene="gcd"
                     /locus_tag="ACHKV2_002453"
     CDS             15862..18252
                     /gene="gcd"
                     /locus_tag="ACHKV2_002453"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO6940325.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18454..20004)
                     /gene="cueO"
                     /locus_tag="ACHKV2_002454"
     CDS             complement(18454..20004)
                     /gene="cueO"
                     /locus_tag="ACHKV2_002454"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO6940326.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20170..20517
                     /gene="yacC"
                     /locus_tag="ACHKV2_002455"
     CDS             20170..20517
                     /gene="yacC"
                     /locus_tag="ACHKV2_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO6940327.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20623..21489
                     /gene="speE"
                     /locus_tag="ACHKV2_002456"
     CDS             20623..21489
                     /gene="speE"
                     /locus_tag="ACHKV2_002456"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO6940328.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21505..22299
                     /gene="speD"
                     /locus_tag="ACHKV2_002457"
     CDS             21505..22299
                     /gene="speD"
                     /locus_tag="ACHKV2_002457"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO6940329.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22337..22699)
                     /gene="yacL"
                     /locus_tag="ACHKV2_002458"
     CDS             complement(22337..22699)
                     /gene="yacL"
                     /locus_tag="ACHKV2_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO6940330.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22875..25472)
                     /gene="acnB"
                     /locus_tag="ACHKV2_002459"
     CDS             complement(22875..25472)
                     /gene="acnB"
                     /locus_tag="ACHKV2_002459"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO6940331.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25827..27680
                     /gene="yacH"
                     /locus_tag="ACHKV2_002460"
     CDS             25827..27680
                     /gene="yacH"
                     /locus_tag="ACHKV2_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO6940332.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27751..29175)
                     /gene="lpdA"
                     /locus_tag="ACHKV2_002461"
     CDS             complement(27751..29175)
                     /gene="lpdA"
                     /locus_tag="ACHKV2_002461"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6940333.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29383..31275)
                     /gene="aceF"
                     /locus_tag="ACHKV2_002462"
     CDS             complement(29383..31275)
                     /gene="aceF"
                     /locus_tag="ACHKV2_002462"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO6940334.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31290..33953)
                     /gene="aceE"
                     /locus_tag="ACHKV2_002463"
     CDS             complement(31290..33953)
                     /gene="aceE"
                     /locus_tag="ACHKV2_002463"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO6940335.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34114..34878)
                     /gene="pdhR"
                     /locus_tag="ACHKV2_002464"
     CDS             complement(34114..34878)
                     /gene="pdhR"
                     /locus_tag="ACHKV2_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO6940336.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8666 a         9250 c         8578 g         8538 t
ORIGIN      
        1 atagattagg cattttttgt ttaaaaaacc tagaatagca acaattcaat acaccacgta
       61 aatatattca cacccgtgat aattcacccc tattacatcc tccaaaaact ttgagtaata
      121 tccgctcgca atattaattt catataattt ttgtaaggat gcatgtaatg aaaaaagcac
      181 ttctcgcagc cgctctggtt atggcttctg gttccgccct ggctgtagat ggtggtcata
      241 tcgactttaa cggtatggta cagtccggta cctgtaaagt gggtgtggta gatactggta
      301 tgcatagcgt taccactgat ggcgtggtta ccctggatac tgcgaatgtt actgatactt
      361 ttgctgaagt tagcgcaact gctgtcggtt tactgccgaa agagttcatg atttctgttg
      421 agtgtgatcc aggtgctccg aagaatgctg agttaactat gggttctgca agttacgcga
      481 acaccagcgg taccctgaat aacaatatga acatcactgt taacggtatt gcaccggctc
      541 agaacgtaaa cattgcagtt cataacatga aaaacaaagc tggcgctgct gaaattaagc
      601 aggtccatat gaacaactct tctgaagttc aggaactgac attagacgca gaaggtaaag
      661 gccagtacgt atttaacgca tcttacgtta aagcaccgaa cagcccggct gtaactgctg
      721 gtcatgtaac cactaacgcg ctgtacaccg ttgcttataa gtaattgatg gtgtgttgag
      781 cataaggttt gccagcaggc aaaccttatt ttcgcgaatt taatctttct acagacagca
      841 aggagtgctt atgtttccct tggttaaaaa aacggttagt gcattatttg tgagtactct
      901 gttagccagt gcaccagcat tcgctgacat tattatttcc ggaacacgta tcatttataa
      961 cgctgataaa aaagatgtta acgttcgtct ggaaaataaa gggaatcgcc ccttactgat
     1021 tcagaactgg ttggatacgg gcgatgacaa tgccgatccc agccagatca aagtgccgtt
     1081 taccgcgacc ccaccagttt cacgcgtgga gccaaaacgc ggtcaaaccg taaaagtgat
     1141 gtataccggt gcaacagccc tgccgaaaga ccgtgaaagc gtctactggt ttaacgtgct
     1201 ggaagtgccg cctaagccga aagatgctga ggctgacaaa aacttgttac agctggcttt
     1261 ccgcacacgc atcaaactct tttatcgccc gagtgggtta cagggagaac cggctgaagc
     1321 accggcgaaa atcacctgga aattaaacaa cgctcagtta caggcaaata acccaacgcc
     1381 atattacgtg tcctttaatg aggtcaagct ggaaagcggc ggcaaaacct acaacgtcaa
     1441 ttccagcatg gtcacgccgt tcagccaggc aagctttggc gtaaccagtc tgccaggaag
     1501 cgtgtcatcc ggaaaagtgg tctttaaagc gatcaacgac tttggcggca acattgatgg
     1561 ttccgcaaca ttctaaaaaa taaacggatt ctttttaaca tcgccaggaa tgcaaccttg
     1621 taccagttta ctcatcaaaa aagccgtatc ccgaaaaaaa cgctacttgc ggcctgttgt
     1681 gccctgtttt atagcagcaa cggtgctgcg gcggacaccg tggaatatga cagttccttt
     1741 ttaatgggaa ctggcgcatc aacgattgat gttaaacgtt atgctcaagg caacccgaca
     1801 ccgccgggtc tctataatgt ccgcgtattt gtaaacggtc aggcgacttc cagcttagaa
     1861 attccgtttg tggatattgg cgaaaacagt gcggcggcct gtcttaccca taaaaacctg
     1921 gcgcaacttc acattaagca acctgaacag cctgtcactt tactcgccag agaaggtgaa
     1981 gaagaggatt gtctggatct ggcaaagtca tacgaaaagg cggatgtgtg ctttgacggt
     2041 agtgaccagt ttctcgatct gacgatccct caggcctatg ttctgaaaag ctatggcggc
     2101 tacgttgacc cttctttatg ggaatcggga attaacgctg ccacactggc atataccctg
     2161 aacgcgtatc acacaagttc agataacgac aatagtgaca gcgtctatgg cgcgttcaac
     2221 tcaggtatca atttaggagc ctggcacttt cgtgcgcgcg gtaactataa ctggacaaca
     2281 gataacggca gcgatttcga tttccaggat cgttacttac agcgtgacat tccggcaatc
     2341 cgttcccaga taattatggg tgatgcctat accaccggtg aaacgtttga ctctgtcaac
     2401 gtccgtggtg ttcgcctgta cagcgacagc cgtatgctgc cttcggcgct ggccagttac
     2461 gctccgacca tccgcggtgt agcaaactcc aacgccaaag tcaccgtgac gcaaagcgga
     2521 tataaaattt atgaaaccac cgttccgccc ggtgaatttg ttatagacga cattagccct
     2581 tccggctttg gtagcgaact ggtcgtgacc attgaagaag cggatggttc caaacgcacc
     2641 tttacgcaac ccttctcgtc ggttgtacaa atgcaacgtc ctggtgtggg ccgttgggat
     2701 ttcagcgcgg gtaaagtcat tgatgacagt ctgcgatccg aacccaatat ggggcaagcc
     2761 tcttattact atggtctgaa taacctcttc acgggttata ccggcattca gttcaccgat
     2821 aataactatc ttgccgggct gttaggtgtg ggtatcaaca ccagcatcgg cgcctttgcg
     2881 gtagacgtta cccattcccg tgctgaaatt ccggatgata aaacctacca ggggcaaagt
     2941 tatcgcgtga cctggaacaa acttttccag gataccggga catcatttaa cctcgcggcg
     3001 taccgctatt ccacccagga ttacctgggc ctgcatgatg cgttagtcct cattgacgac
     3061 gccaagcatt tgtctgccga tgaagacaaa aacaccatgc agacgtactc acgtatgaaa
     3121 aaccagttta ccgtcagcat taaccagcca ttgaatatcg cctatgaaga ttacggttcg
     3181 ctgtttattt ccggtagctg gacgtattac tgggcggcga acaatagccg cactgaatat
     3241 aatgttggtt acagtaaaag cgtttcgtgg ggcagtttca gcgtcaacct acaacgtagc
     3301 tggaatgaag acggcgagaa agatgacgcg atgtacgtca gcgttagcgt acctattgag
     3361 aatattttag gtggcaaacg taagtcttct ggtttccgca atttaaatac tcagctcaat
     3421 accgatttcg atggttcaca tcagttgaat gttaacagtt ccggtaacac tgaaaacaat
     3481 ctggtgaact acagtgtcaa cgcaggttat agcctcgata aaaacgccgg cgatttagcc
     3541 tctgttggtg gttatctcaa ctatgaatct gggttaggcg gtatttccgc ttcggcctcg
     3601 gccacttctg ataacagcca acagtactcc atctcaaccg atggcggctt tgtattacac
     3661 agtggtggtt taacgttcac taacaacagt ttcagcagta acgacacgct ggtgttaatc
     3721 aacgccctag gtgctaaagg cgcacgaatc aataacagta ataacgaaat cgatcgctgg
     3781 ggatatgccg tgacgtcctc tgtcagccca tatcgtgaaa accgggtagg tctgaacatt
     3841 gaaacactgg aaaacgatgt tgaactgaaa agtaccagcg ccaccaccgt accacgtagc
     3901 ggctccgttg ttttgacccg tttcgaaact gacgaggggc gttctgccgt gctgaatatt
     3961 actgccgcca atggcaaatc cattccgttt gctgcggagg tttaccaggg tgaggtgatg
     4021 atcggcagca tgggccaggg tggtcaggca tttgtacgcg gtattaacga cagcggggaa
     4081 ttaatcgtgc gctggtatga aaacaaccaa accattgact gtaagttgca ctaccagttc
     4141 ccggcgcagc cacaaacgca gggaagcacc aacaccttat tacttaacaa tcttacctgt
     4201 caggtagcaa atcactaata tggaaaattc tatgaaaagg atactactaa catccgcgtt
     4261 aataggcctg ggtttaccgg cggttggctc tgcaacagat cttaatgtcg attttacggc
     4321 caccgtatta gccaccacct gcacgataac tatagtggaa gatggtggtc cagcagtgac
     4381 cggtagtaac gatgaataca gcctgacaat tcctgatgtt ggtctggata aagtcgctac
     4441 cgctgcgccc gaagctcagg ccaactttaa attaaaggcc tccgattgca gcaatggtta
     4501 ctcaaagatt tttaccacac ttacaggaac aacggtttcc ggtaagttaa ttgttaatga
     4561 agcaacctct ggcgctgctg gggtggggat ggggattaag cgtagagata ccgcagactc
     4621 gactttcttc acacccaata atactgacaa atttgaatgg agcgccgatg agaaagccag
     4681 cggtgtgcct ctgaccgtag cactgcgcga aacaacggcg ggagcaggac gcaccggtgc
     4741 gtttcaggca aaagccacct ttaacttcac ctacgaataa cagggacagc caccatgaaa
     4801 ttaaaagtca tcgctacact gattgctact gttgccgtgg gtgtaagctt taacagcaat
     4861 tttgcttctg cgagtacaac gtccgcttct ttaaccgtaa acagtaacct gactatgggt
     4921 acctgcagtg ctcagataat ggataatagt aataaagtga tcaatgaagt ggtctttggc
     4981 aatgtttata tttctgaact cggtgcaaaa agcaaagtgc aacagtttaa aattcgcttt
     5041 agcaattgct ctggccttcc ccaaaacagc gcccaaatag tgctggcacc taatggtata
     5101 tcctgtgctg gttctcaatc gtcatcggcg ggtttttcta acaagtttac tgacgctagc
     5161 gcagcaacca gaacggctgt ggaagtatgg actacagata caccggaaag caatggcagt
     5221 acgcaattcc attgtgctca aaagatacca gtgcctgtga cgcttcccgc cgacaccaca
     5281 actcagcctt acgattaccc gttaagtgca cggatgaccg ttgcggaagg tagattggta
     5341 accgatgtaa gaccgggtaa tttccgctct cccacgactt tcacgatcac ttatcagtaa
     5401 tagcggataa ggatattaga atgctatgca ggcatcataa aatcgtacac tttttagggc
     5461 tggcaacagc ccttatcacg ccttttgcct attccggcca ggatgtggat ctcaccgcaa
     5521 agatcgtgcc cagcacctgt caggttgaag tcagtaataa tggcgttgtc gatctcggca
     5581 cagtgacgct ggactatttt gccgacaatg taaccccgac aacggattat gcgggtggca
     5641 aaacgtttaa tgtgaatgtg gtctcttgcg ataacattca aacgacacag tcgcaaatga
     5701 aattagattt tcagccgcag gcgggatcgc ttgcacaggc gaataatcaa atttttagta
     5761 atgaatatga acagcaggct accggggcga aaaacgtcgg cattgtgatt ttttcggccc
     5821 aaccgaatca gcagacattt aacgtgcgag gaacggatgg ttcctcaaca gcaatttatt
     5881 cggtagcacc tggcaatgct gttccctcaa cctggacctt ttattcccgt atgcagcggg
     5941 tgaataatgc ccttcctcct gaatcgggaa tggtgagaag ccaggttatt gttaacgtat
     6001 cttacgaata ataaggataa ttatgcaaag gaaaggcaat aaactgttga ttcagttatg
     6061 cagtgtgata ctgctatttt ttaccacatc ctggtatgca ttggcgaatg aatgttatat
     6121 agagagaaat gctgaagggg attatcacat gaagataagc tctactcagc ttagtctggc
     6181 gtcacaaatg gtcgaggttc cgacagaaat agccgaagct acatgggatg taaatattca
     6241 actaagaggc gatgccatag ggtgtaaatc tcttggggat agtaaggcag ttcactttct
     6301 taatacagct gacccaagtt taatatccac gtacaccaca acgaatggcg cagcgttatt
     6361 aaaaacaact gttccaggca ttgtgtattc tgtcgagtta ttatgcctta gttgtggtgc
     6421 cgcagatgaa cttgatttat ggctacctgc acaaagtggc gcagataact tcataccaag
     6481 cacccagacg aaatgggcct atgagtacag tgatcaaagt tggtatttac gttttcgctt
     6541 attcataact cctgaattta aacccaagaa tggtgtttcc agcggaacaa cgatagcagg
     6601 aaagattgcg tcatggtata taggtaccaa tgaccagccg tggatcaact tttacattga
     6661 caatgactct ttaaagtttt tcgtcgatga accgacctgt gcaacagttg ccctggcaca
     6721 agatcagggc aacgtcagtg gcaatcaggt aacgcttggg aacagctatg tttcggaagt
     6781 gaaaaatggg cttacgcggg aaatcccttt ttctatccgt gctgaatact gttatgccag
     6841 taaaattacg gttaagttga aagcggcaaa taaacccagc gatgccacac tggtgggtaa
     6901 aacgactggc tcggcttcag gcgtggctgt aaaagtaaat tcaacttatg acaatagcaa
     6961 agtattgtta aaagcagatg gtagcaacac ggttgactac aacttcgccg cctggtcaaa
     7021 caacctgctg tttttacctt ttacggcgca gctggtaccg gatggtagcg gtaatgctgt
     7081 cggtgttgga acattttcag gtaacgcgac cttctccttt acctacgaat aagtaaaacg
     7141 tcattccctc ctcatcgcac cagagttctg ctctggtgcg aacatttccc cacactaata
     7201 accattgcct ccctgttttc ggctgttaga atgcgatcaa tctgacttta gccatcagga
     7261 tacgttatga aaccgaccac cattgcttca ttgcagaaat gtaagcagga aaaaaaacgc
     7321 ttcgccacca tcaccgccta cgactacagc ttcgcaaaac tgtttgccga ggaagggctg
     7381 aacgtgatgc tggtgggcga ctcgctgggg atgaccgttc aggggcatga atctaccctt
     7441 cccgttaccg ttgaagatat cgcctaccac actaccgcag tacgtcgtgg cgcgccaaac
     7501 tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca agcctttgaa
     7561 aacgccgcaa cggttatgcg tgccggtgct aacatggtca aaattgaagg cggtgagtgg
     7621 ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg tcacttaggt
     7681 ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg cggcgatgaa
     7741 gcgagcgatc gactgcttag cgatgcatta gccttagaag ccgctggtgc acagttgctg
     7801 gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcgct ggcgatcccg
     7861 gttatcggta ttggcgcagg caacgttacc gatgggcaga tcctcgtgat gcacgatgcg
     7921 ttcggcatta ccggcggtca tatccctaaa tttgccaaaa atttcctcgc tgaaacgggc
     7981 gacatccgcg ctgcggtgcg gcagtatatg gctgaagtgg agtccggcgt ttatccgggc
     8041 gaagaacaca gtttccatta aggagtgacg ttgtgttaat tatcgaaacc ctgccactgc
     8101 tgcgccagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg gtacctacca
     8161 tgggcaacct gcatgatggc cacatgaagc tggtcgacga agccaaagcc cgcgccgatg
     8221 tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggag gacctggctc
     8281 ggtacccacg caccttgcag gaagactgcg aaaagctgaa caagcgcaaa gtggatctgg
     8341 ttttcgcgcc ctcggtaaaa gagatctacc cgaacggtac tgaaacccat acttacgttg
     8401 acgttccagg gctttcgacc atgctggaag gcgccagccg gccggggcat tttcgcggcg
     8461 tgtcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc tgcttcggtg
     8521 aaaaggactt tcagcaactg gcgctgatcc gcaaaatggt tgctgatatg ggctttgata
     8581 ttgagattgt cggtgtgcca attatgcgcg ccaaagacgg tctggcactg agttcccgta
     8641 acggttatct gacggcagaa caacgcaaaa ttgcgcccgg tctgtacaaa gttttaagtt
     8701 cgattgccga caaattgcag gctggcgaac gggatctcga tgaaattatt gctattgccg
     8761 ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc gatgccgaca
     8821 cattgctgga agtctctgaa accagcaaac gggcagtaat tctggtagcc gcctggcttg
     8881 gcgatgctcg cctgatcgac aacaaaatgg tcgagctggc gtaatactta tactctaaat
     8941 aattcaagtt gcaagttgca ggaaggcggc aagcgagtga caaattcgtc aggaacgaat
     9001 ttgcccagcc gcaggctggc cttcggtgag agacatggat gtctctcatt aatccccagg
     9061 agcttacata agtaagtgac tggggttagc gaacgcagac gcagcacatg caatttgaag
     9121 tatgacgagt ataactggcg ctacggctga tggcgccaat tattaatttg ccccacgtca
     9181 tggcgatggc gtctgtaagc aattattcat aacgcagaca acttagttaa tcagcctatc
     9241 aatctcagca agagataaat ttgtcatctc tgcaacgtct tcccgagaca tccctttact
     9301 cagaaaacgc tgggcgaatt cttgcctctc ctcctgtctt ccctgctgaa tccccttctc
     9361 aatccctttc tcttcaaacc actgtgccag cgtcatcata gacttccctc ccgtttccct
     9421 gtctctcagc acaccataaa acaaatccgc ttgttcagta tgaccgcgtt gcagcatata
     9481 gttttgcatg gcaactaact gactttcgct agtgtaccct tcgtcaatca gcgtgaccag
     9541 ttgctccagc aataacatta agtcgcgctg gcgaatatgt ttttgcagta gttcgagaat
     9601 cgcaatccgc cgatgttgca tgatttcgtc atccggtgtg atggtgatat ccaccagcgg
     9661 gaaaggactg ttatagacgc gtcgcgccag ctccggcgag taaaacatat caaaccagca
     9721 cattgagagc ggataaggtg tggcctcgcc ctgataaaag agaatcggca ctaccagcgg
     9781 tagtttgtcg tgatccgcct ccagatgccg atgcatggcg gctatagaat aacgcatcat
     9841 gcgaaaggcc attttcttat ccggcttgct ttggtgttca atcacaacat gcagataacc
     9901 gggattgccc tgcatttgca cggaatagag cacgtccgtg ctgtgtcctt taaggctctc
     9961 ttcaatgaaa ctccccgact ctaaatgaag cgtgttgagg tcacaaagtt cgcgtaattc
    10021 cactggcaaa tgtatctcca gaaagtcgcg agccgtctcc gcatgcatta aaaattgttt
    10081 aaataccgcg tcatgcggtg tggtactcgg tgcatccatc ttccctgtct tccgtgttgt
    10141 cttgcgtgaa ggcagtatcc agtcaaggcg tgctatcgtc agccacctct ttattgttta
    10201 ccgagcagcg ttcagagaaa tatcaattcg ttacaggcga tacatggcac gcttcggcgc
    10261 ggtacgcaaa ctgctgatgc aaatgccgtc agatgagtag acactaaaca aaaatcgggc
    10321 aatactgcgt gagaatttcc tcaaagcagg caccgcctgc ttcgttaacg acagggtaga
    10381 aaggtagaag ttatgattcg cacgatgctg cagggcaaac tccaccgcgt gaaagtgact
    10441 catgcggacc tgcactatga aggttcttgc gccattgacc aggattttct tgacgcagcc
    10501 ggtattctcg aaaacgaagc cattgatatc tggaatgtca ccaacggcaa gcgtttctcc
    10561 acttatgcca tcgcggcaga acgcggttcg agaattattt ctgttaacgg tgcggcggcc
    10621 cactgcgcca gtgtcggcga tattgtcatc atcgccagct tcgttaccat gccagatgaa
    10681 gaagctcgca cctggcgacc caacgtcgcc tattttgaag gcgacaatga aatgaaacgt
    10741 accgcgaaag cgattccggt acaggttgct tgattgttat ccctgcggct ggttactcac
    10801 cagccgcgac atcgtctcca gcgaatccgt tcttaagata taaagtcgtt ttagtaacaa
    10861 cggattatcc cccggtttta ctttgccttt catggttgtc accgccaggt gaaatccggc
    10921 atcgtttgct gccttcacgg ctttgtcatt aaagccgcca aacggatacg aaagatacca
    10981 gacatgcgga ttaaattgcg ccagagcgcg acgtgaacgt gcaaaatcaa acagaatatt
    11041 gtgctcacta cggctcagta atatgggtcg gcgataacca tctacccgat gcaaaaaatg
    11101 ggtatgtgac tggaaatcaa atacatcgcg aatttcgtta agctcagaaa cgctcataaa
    11161 ttgcagcgat tttggttccc acttctgcgg gtgacgtttg atgcgcgagg taacaataaa
    11221 cgccgtcgcc ttcatgccat attgtttcaa cacaggatac gcatagcggc tcactgactt
    11281 gaggccatca tcaaaggtaa tcaccaccgc tcgcgcagga agattgatct tattcttcac
    11341 gtaaccttcc agctgcacca tgctcagtgt cgcgtatccc ctgtcgcgca gccaggccat
    11401 ctggttattg aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac gggtgttttc
    11461 ttcgtcgcgc agaatatggt gataggttag caccggaagg ccattatcgg gttgggcatc
    11521 cagcgcgctg atataggcca gtcgatcgcc aatacggatc tgataccagg tttgatttaa
    11581 cctgtctttc agtttatgca aaatcgggta gcgcaaattg tccgccagta ccccaaatgg
    11641 cgcactgccc acactcggcg cgttatagac cggcgtatct ttccaggtaa ctaagttctg
    11701 attactcagc ggcttgttga ggtcacccaa accgtcttca actttttgtc gcccctgaac
    11761 cggctcaaga tgacctttat cgataaaccc tttatcaaag ccaaaattaa atgcgtaata
    11821 acttgcggca gtgggctcca cggcaatgat ttgtccggcc cgaatattcc ccacggtcac
    11881 catcttgtca ccaatttgcg cccagaccgc agcattttcg atggtttgca tataacgggc
    11941 aggtaacgcg gcactgacac ttgcggtaaa cagcatcagc aggagaataa cagcttgttt
    12001 gtacatgaaa aaatcaccga gggtgaaaca acgcttttat tgtagcaaaa gcgccatcag
    12061 aatcgcgggt taatactcat acaaattatg tttgaggacg aaaggcggat ttttttgttg
    12121 ctggtgccaa atactactca cctccggcgc acccagttcg acaatacgct cgcgaaactc
    12181 tgaactggtc atggtttcac ggcaagccat catctgttca attaacggta acgtgacacc
    12241 agaaatcact tcgcaacggg agtgtttgtg gcttaataat gaagccacgc ggtacggtgg
    12301 cgctccggct atatcggtta agaagatgac accctcacca gagtccgctt catgcagagc
    12361 atcgcacatc atgcggctga gcatattaga acttaatccg cgccagaaat tcacggcccg
    12421 gcactgaaga agcgccccat gttttttctc cagcacatcc agtatctctt gcgcccggtc
    12481 atcgtgacag gtaattaccc aacctaacat cgccttcctc cttagccgaa aatcagtcta
    12541 ccctgaccat tgttgataaa gggtgatagc cgtcaaaact cctctccccc aaatccagag
    12601 gagaggaaat agccttagct acgcaaacca cgtccacgtt ggatcagcga ccaacagatc
    12661 aaataaaacg ccacaataaa gaccaccagt acgccaaagg tagtgaccag cggaacatca
    12721 ttgataccga ggaagccgta gcggaaacca ctgatcatat aaacgattgg gttcaggtgc
    12781 gacagccctt gccagaacgg cggcaacaaa gtcagtgagt aaaagacccc acccaaatac
    12841 gtgagtggcg ttaacacaaa ggttggcacc aggctgatgt catcgaacgt tttggcaaac
    12901 acaccgttca gcaaacccgc aagggagaac aacaccgccg tgagcaccag ggttaaggca
    12961 acgaataccc acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc cgtcaccaga
    13021 atgccaacaa acagaccacg cgccacgcca ccgccgacat atccggcaat aatgacgtga
    13081 gtcggaaccg gcgctaccag cagctcttca atattacgct ggaacttggc accaaaaaat
    13141 gatgacgcga cgttggcgta ggcgttggtg atcaccgaca tcatgatcag ccccggtacg
    13201 atgaactgca tgtagctgaa gccatgcata tcgccaatac gcgaaccaat caggttaccg
    13261 aaaataataa agtaaagggt catggtgatg actggcggca ccagcgtctg cacccagata
    13321 cgcataaagc gatggatctc tttcgcccag atgcttttta gcgccaccca gtaaagatgc
    13381 atcatgcgcg atctccttgt ttttcattaa ccagtgaaac aaacagctct tccagacggt
    13441 tagctttgtt acgcatactt aatacctgaa tgccctgctc acttaactgc gtaaatacgc
    13501 tgttgatccc ctgctcacgc agcacttcaa cttccagcgt cgcggtatcg accagtcgat
    13561 actgatagcc atcgagcttc ggtaacggac ttttcggtgc gagatcgaga ataaaggttt
    13621 ccgatttcag cttcgccagc agcgccttca tcgaggtatt ttccaccagc tcaccgtgtt
    13681 gaataatgcc gatattgcgg cacagcattt ctgcttcttc caggtagtgt gtggtgagaa
    13741 tgatggtggt gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag cggcgaagtt
    13801 caatatccac gcctgcagtc ggctcgtcga gaatcagtag tttaggttca tgcattaacg
    13861 cacgggcaat cattaaacgg cgcttcatcc cgccagataa catgcgcgca cgttcgttgc
    13921 gttttcccca taaatcgagc tgcttaagat atttttcgct gcggatgtac gcctctttgc
    13981 gctccacacc gtagtaccct gcctgattca ccacaatttg ctgcacggtt tcaaacgggt
    14041 tgaagttaaa ttcctgcggc accagtccca actgacgttt agcgttcacg acatctttct
    14101 cgagatcgta accaaatacg ctgacccgcc cggaggtttt atttaccaga gagctgataa
    14161 taccgatagt ggtcgatttc ccggccccgt tcggcccgag aagcgcataa aaatcacccg
    14221 cttcgacctg caaatctatt ccacgaagcg cctgaacgcc gcctggatag ttttttttaa
    14281 gctgttgaag ttccagtgca atggtcataa atttttactt accttacgtt cttacacttt
    14341 atctgtggtt taaatcgtcc cggagttgcc ctatattagc caaacgtaat tatttggtta
    14401 caggtcgtta acctccatga aagacataga tacactcatc agcaacaatg cactatggtc
    14461 aaaaatgctg gtggaagagg atcccgggtt ttttgagaaa ctggcacaag cgcaaaaacc
    14521 gcgctttcta tggattggat gttccgacag tcgcgttcct gcagaacgtt taaccggtct
    14581 tgagccgggc gaactctttg ttcaccgtaa tgttgctaac ctggtcattc acaccgacct
    14641 gaactgcctt tccgtggttc agtatgcagt ggatgtactc gaagttgaac acattattat
    14701 ctgtggccac tacggttgcg gcggcgtaca agccgcagtt gaaaacccgg aactggggct
    14761 tatcaacaac tggctgctgc atatccgcga tatctggttc aaacatagct cattgctcga
    14821 cgaaatgccg caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca tggaacaggt
    14881 gtataacctg ggccactcca ccattatgca atcagcgtgg aaacgcgggc agaaagttac
    14941 cattcacggc tgggcttatg gcattcacga cggcttgctg cgtgatctgg atgttactgc
    15001 caccaaccgc gaaacccttg agcaacgtta ccgtcacggg atttccaacc tcaagctgaa
    15061 acacgccaac cacaaataaa aatgccatgc cggatgcaac acatccggca acttcacact
    15121 tactcgtcca gcagaatcac tttgccgata tacggcagat gacggtaacg ctgtgcgtaa
    15181 tcaatgccgt aacccaccac aaactcatcc gggatcgaga aaccgataaa ttctaccggg
    15241 acgttcactt cacgacggga cggtttatcc agcagcgtac aaatcgccaa cgacttcggt
    15301 tcgcgcaggc ttaagatctc acgcactttc gacagtgtat tccccgagtc gatgatatct
    15361 tcaacaatca gcacatcctt gccacggata tcttcatcca gatctttgag gattttcaca
    15421 tcacgggtgg tggacatgcc gctaccgtag ctggaggcgg tcataaagtc gacttcatga
    15481 gatacctgaa cttcacggca caggtccgcc ataaacataa atgagccacg cagcagaccc
    15541 accagcacca tatcgctgcc gctgtctttg taacgctcag taatctgacg acccagttcg
    15601 gcgatacgcg ctttaatctc cgcttcgggg atcattactt ctacagtatg tttcatatct
    15661 ctaaccatat gatttaaaaa caaatcactt cgcacgggca ggttttggca ccgcaacaaa
    15721 gcgcaagaca cgcagtatac cagcaaaacg attcattcgc ggaatctgtt aataaagctc
    15781 aaattgtgat gacgatcaca catgttaaaa cctataatta attgctacta aaatattaat
    15841 gaattgaaat ggtgtctctt tatggcaatt aacaatacag gctcgcgacg attactcgtc
    15901 acgctaacag ccctttttgc agcgctttgc gggctgtatc tactcattgg cggaggctgg
    15961 ctggtcgcga ttggcggctc ctggtactac cctatcgctg gccttgtgat gctcggcgtc
    16021 gcctggatgc tgtggcgcag taaacgtgcc gcgctttggc tatacgccgc cctgctgctc
    16081 ggcaccatga tttggggcgt ctgggaagtt ggtttcgact tctgggcgct gactccgcgc
    16141 agcgatattc tggtcttctt cggcatctgg ctgatcctgc cgtttgtctg gcgtcgcctg
    16201 gtcattcctg ccagcggcgc agttgccgca ctggtggtgg cactgctgat tagcggtagt
    16261 atcctgacct gggccggatt taacgatccg caggaaatca acggcacctt aagcgccgat
    16321 gccacacctg cggaagctat ctcacccgtt gccgatcagg actggcctgc ctatggtcgc
    16381 aatcaggaag gtcaacgctt ttcacctctg aaacaaatta acgccgataa cgtccataat
    16441 ctgaaagaag cctgggtgtt ccgtactggc gatgtgaagc agccgaacga tccgggcgaa
    16501 atcaccaatg aagtgacgcc gattaaagtg ggcgacaccc tttacctgtg taccgctcac
    16561 cagcgcctgt ttgcgctcga tgccgccagc ggcaaagaga aatggcatta cgatcctgag
    16621 ctgaaaacta acgagtcttt ccagcacgta acctgccgtg gcgtctctta tcatgaagcc
    16681 aaagcagaaa cggcttcgcc ggaagtgatg gcggattgcc cgcgtcgtat cattcttccg
    16741 gtcaatgatg gtcgcctgat tgcgattaac gctgaaaacg gcaagctgtg cgaaaccttc
    16801 gccaataaag gcgtgctcaa tctgcaaagc aatatgccag acaccaaacc gggtctgtat
    16861 gaaccgactt cgccaccgat tatcaccgat aaaaccatcg tgatggcagg ttcagttacc
    16921 gataacttct caacccgcga aacgtctggc gtgatccgtg gttttgatgt caacaccggg
    16981 gagctgctgt gggcttttga tccgggcgcg aaagatccga acgcaatccc gtctgacgaa
    17041 cacaccttta cctttaactc gccaaactcg tgggcaccag cggcctatga cgcgaagctg
    17101 gatctggtgt atctgccgat gggcgtgacc acgccggata tctggggcgg taaccgcaca
    17161 ccggaacagg aacgttatgc cagctcgatt ctggcgctga atgccacaac cgggaaattg
    17221 gcgtggagct accagaccgt tcaccacgac ctgtgggata tggatcttcc ggcacagccg
    17281 acgctggcgg acatcaccgt taatggtcag aaagtgccag ttatttacgc tccggcgaaa
    17341 accggcaaca tttttgtgct cgatcgtcgt aatggcgaac tggtggttcc ggcaccggaa
    17401 aaaccggttc cccaaggtgc tgccaaaggc gattacgtta cccctactca accattctcg
    17461 gaactgagct tccgtccgac gaaagatttg agcggtgcgg atatgtgggg agccaccatg
    17521 tttgaccaac tggtgtgccg cgtgatgttc caccagatgc gctatgaagg cattttcacg
    17581 ccgccatctg aacagggcac gctggtcttc ccgggtaacc tgggaatgtt tgaatggggc
    17641 gggatttccg ttgatccaaa tcgtgaagtg gcaattgcca acccaatggc actgccgttt
    17701 gtttcgaaac tgatcccacg cggtccgggt aacccgatgg agcagccgaa agatgccaaa
    17761 ggcacgggta cggaatccgg cattcagcca cagtacggtg taccgtatgg tgtcacgctc
    17821 aacccgttcc tctcaccatt tggtctgcca tgtaaacagc cagcatgggg ttatatctcg
    17881 gcgctggatc tgaaaactaa tgaagtggtg tggaagaaac gtattggtac gccgcaggac
    17941 agtatgccgt tcccgatgcc tgtaccggtg ccgttcaata tgggtatgcc gatgctgggc
    18001 gggccaatct ccacggcggg taacgtgctg tttatcgccg ctacggcaga taactacctg
    18061 cgcgcttaca acatgagcaa cggtgaaaaa ctgtggcagg gtcgtttacc agcgggtggt
    18121 caggcgacgc caatgaccta tgaagtgaat ggtaagcagt atgtggtgat ctccgcaggc
    18181 ggtcacggtt catttggtac gaagatgggt gactatattg tggcttatgc gctgccggat
    18241 gatgtgaagt aagacttgct cagattgctg acaacgtgcg cgttgttcat gccggatgcg
    18301 gcgtgaacgc cttatccagc ctacaaaatt gtgcaaaatc aatggattgc acaggtaacg
    18361 taggcctgat aagcgtagcg catcaggcag atttgcgttt gtcatcagtt taatgcccgg
    18421 agagatccgg gcatattccc gaatacggtc tttttatacc gtaaacccta acatcatccc
    18481 cgtatcttca tgctccagca gatggcagtg cgccatataa gcacgttctt tcggtgcgtc
    18541 gtgattaaac ttcaccagca cttcactgac attgccttct accttaacgg tatctttcca
    18601 gcccgcgcga tgagccgctg gcggtttgcc attttctgac aagatacgga actgcgtgcc
    18661 gtgaatatgg aacggatgca gcatcatgtc gcccacgcca gagataaccc aacgttcgta
    18721 ctgccctttc gccgccgcaa acatcggctt attcatatca aacgcctgac cgttgatttt
    18781 attggcatgg tggaaatcga acttcccgcc gtggttcata tgattcatat tgccgtgccc
    18841 catatggccc atcatctggc tgtgatccat tccggccatc gcctgatcgc catatttctc
    18901 cattagcatc tgcatcccca tcatatcgag catcgggtcc atagaaagtt gcagcttgcg
    18961 taccgtcagc ccttccagcg aaggtaacgc aggcaggcta cttaatgtgt ctggcaaagc
    19021 accggaagca ctaatagcaa tcggctgaat ccgcattacc ggatgaggct tatcaaacgg
    19081 cgcaatcgcc atccccatct ggctgaccgg cagcgtcacc aggtcaaagg gtttgttgtc
    19141 gttaacctcc accagcactt caaaacgctc gcccatcagc accggcagct cgttcacctt
    19201 caccggttca ggtagcagac caccgtcgct ggcaatcaca taaagcgggc gattgtcgct
    19261 ggtggcgaaa ttgagcgagc gggcattaca gccattgagc aaacgcaggc gcagccaacc
    19321 acgcggggca gcgtgttgcg ggtagattgc accgttggtc agcaacgtat cgccaaacca
    19381 gcccacggcg gcggtcatca catccagttg ataatcaatc tgcccgtcgg cgctaaattt
    19441 cttatcctga acgatcaccg gaacatcatc gataccccac tgttttggca gcattaattt
    19501 caggatctcg tcatcttcaa tcaccaccag accagccagc cccatcgcca cctgtcgccc
    19561 ggtcttgcca tgttgatgtg gatggaacca gcaggtagcg gcaggttgat caacgttcaa
    19621 cgtcaccgag cgcttgccac ctggcggaat aattccctgt gggccgccgt cgacttcacc
    19681 cggtacttcc agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt tatagatatc
    19741 aaccgttacc gctttgccgc gctgtaattt caccgccggc cccagcagat tgccgttata
    19801 gccccaggta gttgcagttt tctcgccaaa ggtagactga cctgcgccaa tagttaactg
    19861 aatgcgatta cgggcatcgg tcgtgagcaa atcagggatc ggtaacgttg ggcgttccgc
    19921 cgcaaagact gcacggctcc acagtggcaa ggctgaagcc acacccagtg cgacggaata
    19981 tttcaagaaa tcacgacgtt gcatagttat ttccttattc ttaagcaggc gaaacaaaat
    20041 caaacgttga gcatagtcct tccccttacg ggaaggtcaa gccgcagaca caatttaatc
    20101 gacaataatg gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt taagcccctg
    20161 gtagaagcaa tgaagacgtt tttcagaaca gtgttattcg gcagcctgat ggccgtttgc
    20221 gcaaacagtt acgcgctcag tgagtctgaa gccgaagata tggccgattt aacggcagtt
    20281 tttgtctttc tgaagaacga ttgtggttac cagaacttac ctaacgggca aattcgtcgc
    20341 gcactggtct ttttcgccca gcaaaaccag tgggatctca gtaattacga caccttcgac
    20401 atgaaagctc tcggtgaaga cagctaccgc gatctcagcg gcattggcat tcccgtcgct
    20461 aaaaagtgca aggccctggc tcgcgattcc ttaagcctgc ttgcctacgt caaataatcc
    20521 ctgatacctt tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag ctattatgtt
    20581 gcgccctttt tttacgggtg ttaacaaagg aggtatcaac ccatggccga aaaaaaacag
    20641 tggcatgaaa cgctacacga ccagtttggg cagtactttg cggtagataa cgttctgtat
    20701 catgaaaaga ccgatcacca ggatctgatc atttttgaga acgctgcatt tggtcgcgta
    20761 atggcgctgg atggcgtagt acaaaccacc gagcgcgacg agtttatcta tcatgagatg
    20821 atgacccatg ttccgctact ggcccacggt cacgcgaaac atgtgctgat tatcggcggc
    20881 ggcgacggtg ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc aatcacgatg
    20941 gtggaaatcg atgcgggtgt cgtgtcgttc tgccgtcagt atctacccaa ccataacgcc
    21001 ggtagctacg acgatccgcg ctttaagctg gtgatcgacg atggcgtcaa tttcgttaat
    21061 caaaccagcc agacctttga tgtcattatc tccgactgca ccgatcctat cggtcccggc
    21121 gaaagccttt tcacttcggc attttatgaa ggctgcaaac gttgcctgaa tcctggcggt
    21181 atcttcgtcg cacaaaacgg cgtctgcttt ttacagcagg aagaagccat cgacagccat
    21241 cgcaaactca gccattactt cagcgacgtt ggcttttatc aggcggcgat cccgacctat
    21301 tacggcggta tcatgacttt tgcatgggcg acagataacg acgccttacg ccatctctca
    21361 accgaaatta ttcaggcgcg ttttctcgcc tctggcctga aatgccgtta ttacaatccg
    21421 gcagtccata cggcagcttt tgccttaccc cagtatctgc aagacgcact ggcttcacag
    21481 ccgtcctaag gagaagataa gaaattgaaa aaactgaaac tgcatggctt taataatctg
    21541 accaaaagtc tgagtttttg tatttacgat atctgctacg ccaaaactgc cgaagagcgc
    21601 gacggttata ttgcttatat cgatgaactc tataatgcca accgtctgac cgaaatcctg
    21661 tcagaaacct gttccattat cggggctaat attcttaaca tcgcccgcca ggattacgaa
    21721 ccacagggtg ccagcgtcac tattctggtg agtgaagaac cggttgaccc gaaactcatc
    21781 gacaaaacag aacaccccgg cccactgcca gaaacggtcg ttgcccatct cgataaaagt
    21841 catatttgcg tacataccta cccggaaagt catcctgaag gcggtttatg taccttccgc
    21901 gccgatattg aagtctctac ctgcggcgtg atttctccgc tgaaggcgct gaattacctg
    21961 atccaccagc ttgagtccga tatcgtaacc attgattatc gcgtgcgcgg ttttacccgc
    22021 gacattaacg gtatgaagca ctttatcgac catgagatta attcgattca gaactttatg
    22081 tctgacgata tgaaggcgct gtatgacatg gtggatgtaa acgtctatca ggaaaatatc
    22141 ttccatacca agatgttgct taaagagttc gaccttaagc actacatgtt ccacaccaaa
    22201 ccggaagatt taaccgacag cgagcgccag gaaattaccg ctgcgctgtg gaaagaaatg
    22261 cgcgagattt attacgggcg caatatgcca gctgtttaac ggctctggcg gagctcccag
    22321 gctccgccag atttatttac ttctgctgca cgaaattgcg gtaagccgcc acgacctgca
    22381 gaaaatcctc aacgccgcat agcgacaggc tttcttcgtc gtagtagttc atcccctctt
    22441 ccatttcatc gccagcgaat tccagttgat tggcgcgaac catcacctct tcgccgtcca
    22501 tccatagcgt atactcatgc cctgcccgct gccaggaacg ttcgctacct ttcagtgcgt
    22561 gcgctgcttg ttccacttca tcaagcaagg ccaggttttc tttcacctct tcattaaacc
    22621 agtgcccgac cacttcatgc cccatggaca tacgcacctt taccactccg gtaatatcgc
    22681 gcagaaattc gtaatccata gtgtgttcct ctgctcccgg caatgcaccg aaatcgcatt
    22741 gcgccattga tgtaattatc gcagccttac cggagaaaaa cagcggaacg gcgatggttt
    22801 agaaataaaa aaatgcccgg gaatctcatt caccgggcat tgtgtcgttt atgcgcagcg
    22861 cgtgcgctga ctttttaaac cgcagtctgg aaaatcaccc catcggcttt ctcggtgtac
    22921 tgagaaagct ggttgaagtt cagataacgg taagtatcaa cggctgtttt atctacctgc
    22981 gccacgtagg tctggtactc ttccggcgtc ggcagtttgc caatcagcgc cgcaacagca
    23041 gccagttccg cagaagccag gaagacattc gcgccagtac ccagacggtt cgggaagtta
    23101 cgggtagagg tggaaaccac cgttgcaccg tccgccacac gcgcctggtt acccatacac
    23161 agggaacagc cagggatctc gatacgcgca ccactcttac cgaagacgct gtagtagcct
    23221 tcttcggtca actgtgcggc gtccatacgg gttggcggtg ccacccacag gcgggtcggc
    23281 aactgacctt tgtgcgcatc cagcagttta cctgcggcac ggaagtgacc gatgttagtc
    23341 atgcaagaac cgataaacac ttcgtcgatc ttctcgccct gtaccgcaga cagcggacgt
    23401 gcgtcgtccg ggtcgttcgg agcacagagg attggctctt taatatccgc cagatcgatg
    23461 tcgatcactg ccgcgtattc cgcatctgca tcggcttcca gcagctcagg attcgccagc
    23521 catttttcca tgccctgaat acgacgttcc agggtacgac gatcgccgta accttccgcg
    23581 atcatccact tcagcaggac gatgttggag ttcaggtatt cgatgattgg ttctttgttc
    23641 agcttgatgg tacaaccggc agcagaacgc tcggcggacg catcggtcag ctcaaaggcc
    23701 tgctcaactt tcagatccgg cagaccttca atttctagga tgcggccaga gaagatgttt
    23761 ttcttgcctt tcttctcaac ggtcagcaga ccttgtttga tcgcatacag cggaatggcg
    23821 tgtaccagat cgcgcagggt gatgcccggc tgcattttgc ctttgaagcg caccagaacg
    23881 gattccggca tatccagcgg cattacgcct gttgcggctg caaatgccac cagaccagaa
    23941 cccgccggga aagagatacc gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg
    24001 gtatccggca gcagcatacg gttcagccag gagtggatta cgccgtcacc cggacgcagc
    24061 gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaact
    24121 ggcttcggat acgccgcggt gtggcagaaa gactgcatca ccaggtcagc cgagaagccc
    24181 aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc ctgtggtgtc ctgagagcct
    24241 acagaagtca ttttcggttc acagtacgcg cccggacgaa tgcctttcac gccacaggca
    24301 cggcctacca ttttctgcgc cagcgagaag ccgcgatcgc tctcagcgac atctttcgcc
    24361 tgacggaaca catcactgtg cggcagacca agtgcttcac gcgctttggt ggtcaggcca
    24421 cgaccgataa tcagcggaat acggccgcca gcacgcactt catcaatcag cacgtcggtt
    24481 ttcagttcga aggtcgccag cagttcgccg gtttcgtggt tacgtacttc acctttgtac
    24541 gggtaaacgt caattacgtc gcccatgttc aggttagaga catccacttc gattggcagc
    24601 gcacccgcat cttccatcgt gttaaagaag atgggtgcaa ttttaccgcc gaggcacaaa
    24661 ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc ccataaacca cagaacggag
    24721 ttcgtggcgg atttacgcga tgaacccgta cccacaacgt caccgacgta cgccagcggg
    24781 aaacctttct gttgcagagc ttcaatctgt ttgatcgggc caacaacacc tggctggtct
    24841 ggctcaatac cttcacgggc gtttttcagc atcgccagcg cgtgcaatgg gatatccggg
    24901 cgtgaccacg catccggtgc cggagaaagg tcatcggtgt tggtttcgcc agtgactttg
    24961 aagacggtaa cggtcagttt ttcagccagc gccgggcgat tcaggaacca ttcggcatca
    25021 gcccaggact gcataacctg cttcgcatat tcgttgcctg ctttcgcttt ctcttctacg
    25081 tcatagaagt tatcgaacat cagtagtgtg tgagaaagtg ctttggcagc gatcggtgcc
    25141 agtttggcat catccagcgc atcgatcagc ggatgaatgt tgtaaccacc ctgcatagta
    25201 cccagcagtt cgatggcttt ttccggagtc agcagtgggg atttggcttc gcctttggcg
    25261 acagcagcca ggaagcctgc tttaacatag gcggcttcat cgacgccagg aggaacacgg
    25321 ttggttaaca gatctaacag gaattcttct tcgcccgcgg gcgggttttt cagcagctct
    25381 acaagcgcgg ccatttggtt tgcatccagg ggtttgggcg caatcccctc agcggcacgc
    25441 tcagctacgt gcttacggta ttcttctagc acgacggttc tcctcgctct cattgtcata
    25501 gtgcggcagg cgattctctt cacgctcctg tgagacagca gtttgtaggg taaatgccca
    25561 gtaccgcaac gggcagaata gcaggatttt gacgacgtgt taatctgttt acaaaaaagc
    25621 aacataaaaa agtggctgaa tcgttaagga tggtctagag attgtttccc tccgtaaaaa
    25681 cttcgttaat tcgcatggtg ataatcacct ttctcaacga atcccaagat tagtcaaaat
    25741 ttaaactacc gcctctttat actcggattc acagcacctg cgggtggcag ttcgccgacc
    25801 attgcgattt ccttgagatc cgaattatga aaatgacttt gccgtttaaa ccccatgtgc
    25861 tggcgctaat ttgcagtgcc gggctttgtg ccgcctctgc cgggctatat ataaaaagcc
    25921 gcacagtgga agcgcctgtg gaaacgcaat cgacacaact ggctgtgtct gacgctgccg
    25981 cagttacgct tcctgcaacg gtttccgcac ctcccgtaac acccgccgtc gtcaaatccg
    26041 cattcagcac tgcacaaata gatcaatggg tcgcgcccgt cgcgctgtat cccgacgccc
    26101 tactttcgca ggtgctgatg gcatcaacct atccgacaaa cgttgctcaa gcagtgcaat
    26161 ggtcgcacga taatccactt aaacaaggcg atgctgctat tcaggcggta tctgaccagc
    26221 cgtgggacgc cagcgttaaa tcactggtgg cctttccaca attgatggca ttgatgggcg
    26281 aaaacccgca atgggtgcaa aacctgggcg atgcttttct ggcccagccg caggacgtga
    26341 tggactcggt acaacgattg cggcaactgg cacaacaaac cggctcgctg aagtcatcaa
    26401 ccgaacagaa agttattacc acaacgaaga aaactgtacc ggtaacacag acagtcacgg
    26461 ctcccgtcat accatccaat accgtttcaa ctgccaaccc tgtcattaca gagcctgcaa
    26521 caaccgtcat ttccattgag cccggcaatc ctgatgtggt ctatattccc aactacaacc
    26581 caaccgtggt ttacgggaac tgggccaata ctgcgtatcc gccggtttat ctgccaccac
    26641 cagccggaga accgtttgtt gacagctttg tacgcggatt cggctatagc atgggcgttg
    26701 ctaccacgta cgcactattc agcagcatcg actgggatga cgacgatcat gaccatcatc
    26761 atcatgacga tgataattat catcaccacg atggcggtca tcgtgacggt aatggctggc
    26821 aacataacgg cgacaacatc aatatcgacg tcaacaattt caaccgtatc accggtgagc
    26881 atcttactga taagaatatg gcatggcggc acaatccaaa ctaccgtaat ggtgtgccct
    26941 atcatgatca ggatatggca aagcggtttc atcaaactga tgtcaacggc ggaatgagtg
    27001 ccacgcagct acctgctcca acacgcgaca gccagcgtca ggcggcagca agtcagtttc
    27061 agcaacgaac acacgccgcc cccgtcatta cacgagatac ccaacgtcag gcagcggcac
    27121 agcggtttaa tgaagctgaa cactatggga gctatgacga cttccgcgac ttcagccgtc
    27181 gccaaccact gacccagcaa caaaaggacg ccgctcgtca gcgttatcag tcagcttctc
    27241 ctgagcagcg ccaggcagtt cacgagaaaa tgcagactaa cccgcagaac cagcagcgaa
    27301 gagaggcagc gcgtgagcgc attcagcccg cctcgcctga gcagcgccag gcagtccgcg
    27361 agaaaatgca gactaaccca cagatccagc agcgaagaga cgcagcgcgt gagcgtattc
    27421 agtcagcctc gcctgagcag cgccaggtgt ttaaggaaaa agtacagcag cgcccactga
    27481 accaacagca acgtgataac gcccgccagc gtgttcaatc agcatcacct gaacaacgtc
    27541 aggtttttcg ggagaaagtt caggagagcc gcccacaacg tctaaacgac agtaaccata
    27601 ctgccaggct gaataacgag caacggtcag cagtacgcga acgtctctct gagcgcggag
    27661 caaggcgact ggaaaggtaa attacaggcg taaaaaaagc ggcgtggtta gccgcttttt
    27721 taattgccgg atgttccggc aaacgaaaaa ttacttcttc ttcgctttcg ggttcggcag
    27781 gtcagtaatg ctaccttcga acacttctgc cgccaggccc acagactcgt gcagagtcgg
    27841 gtgcgcgtgg atggtcagtg cgatgtcttc agcatcacaa cccatttcaa ttgccaggcc
    27901 gatttcaccc agcagctcgc cgccgttagt accgacaatc gcaccaccga tcacacggtg
    27961 agattctttg tcgaaaatca gcttggtcat accgtctgcg cagtcggaag cgatagcacg
    28021 accagaagca gcccacggga aggtggcggt ttcatagctg atgcctttct ctttcgcttc
    28081 tttctcagtc agacctaccc atgcaacttc tggttcggta taggcgatgg acgggataac
    28141 tttcggatcg aagtagtgtt tcttaccggc gataacttca gcggcaacgt gaccttcgtg
    28201 aacacctttg tgtgccagca tcggctgacc gacgatatcg ccgatagcaa agatgtgcgg
    28261 tacgttggta cgcagctgtt tgtcaacgcg gatgaaacca cggtcgtcaa cttccacgcc
    28321 agctttgcct gcgtcgaggt ttttaccgtt cggcacacga ccaatcgcta ccagcacggc
    28381 gtcgtaacgc tgcggttcag ctggtgcttt tttgccttcc atcgtcacat aaataccgtc
    28441 ttctttcgct tcaacggcgg taactttggt ttccagcatc aggttgaatt tcttgctgat
    28501 acgcttggtg aagactttaa cgatgtcttt gtcagctgcc gggataacct ggtcgaacat
    28561 ttcaaccacg tcaatctgtg aacccagcgc gtggtaaacg gtgcccattt ccagaccgat
    28621 gataccgcca cccattacca gcaggcgttc tggtacttct ttcagttcca gcgcgtcagt
    28681 agagtcccag atacgcggat cttcatgcgg aataaacggc agttgaatcg ggcgagaacc
    28741 cgctgcaatg atcgcgttgt cgaagttgat cacggtttta ccgttctcac cttcaacttc
    28801 cagggtgtta gccccggtaa atttacccag accgttgacc actttgactt tgcggccttt
    28861 cgccatacca gccagaccac cggtcagctg attgatcact ttctctttcc aggtacgaat
    28921 cttgtcgata tcggttttcg gttcgccgaa gacgataccg tgttcagcca gcgctttggc
    28981 ttcttcgata acttttgcta cgtgcagcag tgctttagaa gggatacagc cgacgttcag
    29041 gcaaacaccg ccaagggtgt tgtaacgttc tacgattacg gtttccagac ctaaatcagc
    29101 gcaacggaag gcagcggagt aacctgcggg gcctgcccca agtaccacga cctgagtttt
    29161 gatttcagta ctcatcatga cctctatata tttatctccg gcgggtctga cgtttttttt
    29221 atgacgccca ttatccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa
    29281 tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg
    29341 agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg
    29401 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
    29461 cacgcggtgg tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc
    29521 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt
    29581 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc
    29641 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
    29701 ctcgcgagac agttcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag
    29761 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
    29821 accgtcttcc gacagcgaac tgttgaagcg aggcatctgc tcaagcgctg cagcaacggc
    29881 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc
    29941 gttctgctgt ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgagtaac
    30001 atgcgggatc atcacccagt tacggctcag gttcgcacca gaaattttct ggatacggcc
    30061 cagttccact tcttcgattt caccaaactt gctgaagtcc accttcggcc acggcagcat
    30121 gccagggata ccaccgccag tcgctgccgg agctgcttct gcacgtttga tagcttcttt
    30181 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
    30241 cgcaaggtta acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta
    30301 agcgtcattt tcagcaaatt cagatttgcc ttccgctttc gcagccggtg ctgctgccgg
    30361 ggcttcagct tttgctgccg gagccggcgc tgctgcttcc tgtttcgcag gagctgccgc
    30421 aggcgctgcg ccttcaactt cgaagatcat aatcagcgag ccagttttca ctttatcgcc
    30481 aacgttgact ttcagttcct tcacgacgcc tgcaaacgga gccggaactt ccatagaagc
    30541 tttatcgcct tctacggtaa tcagtgactg ttcagcggca actttgtcgc ccactttcac
    30601 catcacttcg gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac
    30661 gccagccgct ggtgcagggg ccgctgccgg agccgcttcc tgtttagccg ccggagctgc
    30721 cgcgcctgct tcacccgcca cttcgaagac cataatcagc gagccggtag acactttgtc
    30781 gccggtgttc actttgattt ctttcacggt gcctgcaaac ggagccggaa cttccataga
    30841 agctttatcg ccttctacgg tgatcagcga ctgctcagct tcaactttgt cgccaacttt
    30901 caccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt
    30961 tgccgcggca gcctcgggga ctgctgccgg agctgcttct ttcttctctt ctgcctgagc
    31021 aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg
    31081 agttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagacggaac
    31141 ttccatagaa gctttgtcgc cttctacggt gatcagcgac tgttcggctt caactttgtc
    31201 gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt
    31261 gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca
    31321 tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta
    31381 gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcgaa gtggtgacgc
    31441 aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg gtagtcgtca
    31501 gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccaca
    31561 gccggagcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcggatgc
    31621 agcatgttcc agcgttcaca atcctgacca tcacgcgcca gttcggtgaa ggaggtcacg
    31681 ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg
    31741 tgacgcagga tagaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt
    31801 tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg
    31861 tggtagtttt cgttcagcgt ggtgatgtag tagtaaacgt tctcttgttt ttcaccgtac
    31921 atacgctcca gaccgtcatg cataatgaca gcaacttcgt aagcgtaagc cgggtcgtaa
    31981 gagatacagt tcggaatagt cagcgactga atgtggctgt gaccatcttc gtgctgcaga
    32041 ccttcaccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc
    32101 tggtcgccag ccgcccagca cagatcgccg atacgctgga agccgaacat cgagtaatag
    32161 atgtagaacg gaatcatcgg cagattgttg gtgctgtaag aggtcgccgc tgccagccag
    32221 gaacaacctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct
    32281 ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa
    32341 ataccaatct gacggaacag accttccata ccgaaagtac gcgcttcgtc ggcgatgatc
    32401 ggtaccagac gatctttgat cgacttgttc ttcagcatca cgttcagggc acgaacgaaa
    32461 gcgatagtgg tagagatctc tttgctctgc tcttccagca gcgcgccgaa gtcttgcagg
    32521 cttggcagct caagcttctc ggtgaagttc ggctgacggc ttggcagata accgtgcagt
    32581 ttctgacgct gtgcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac
    32641 ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc
    32701 acaccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg
    32761 cccatgccgt aacctttaat ggtatgagca aggattaccg tcgctttgcc tttggtttcc
    32821 tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc
    32881 cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag
    32941 aagtgttcac gaacgtacgc accatctttc gatttgaagg tctggtagtc gccgtcaacg
    33001 gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag ttcatcccaa
    33061 cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat accttccagt
    33121 tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg
    33181 ataacgaaga ccaggttatc cagtttttca cgggtagcga tggtgatcgc acctttggat
    33241 tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtctgttt agaggtatct
    33301 ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcgga
    33361 cccagaccca tagatacggt cgggaactgc cagaattccg gcatcaattt cgggtgcgga
    33421 taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcttgagtc
    33481 agacgacctt ccaggaaagc acgagcgtac acgcccgggg agatgtggcc ctggaagtaa
    33541 accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca
    33601 taaatggttg cggaagactg gaaggacgcc atgtggccgc ccagttcgag gtcttttttc
    33661 gacgcacgca gaaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc
    33721 agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg
    33781 ctgataccgg tgcctgcggc tacgttgaca ccgcctttgc gggcttcagc aagcagttgg
    33841 tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg
    33901 agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat
    33961 tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccgga
    34021 aagctcaata agacaggttc tacgtttagt tgccgcgctt ttatatgcgc ttgatttaca
    34081 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
    34141 cagagaacgc tcacggcggc tttcttcacg gcttctgtcg agcaaaattt cttcgataaa
    34201 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
    34261 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
    34321 ttcgaagttc tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag
    34381 cagaaccaca ttgtgggccg cttcggtgac ggcaatctga tactggagta cggcgtttga
    34441 ttccgcgtcc agatcgccag actgctgcgc cagttctatg gcgtggtgga gttcacggat
    34501 gcgttccttg tcttcatcgg tactacgcag cgcggcgtaa taagcggcga taccttccag
    34561 ggcgtgtcgt gtttcgagca agtcatactg tgattcagga tggtcggaga gcagctccac
    34621 cagcggatcg ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg
    34681 acgaagcaac aagcccttcg cttcgagacg ttgaatcgcc tcacgcaatg agggacggga
    34741 gacgtcaaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
    34801 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
    34861 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
    34921 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
    34981 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gt
//
LOCUS       DAZFTN010000048        34901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFTN010000048 DAZFTN010000000
VERSION     DAZFTN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..786)
                     /gene="rlmB"
                     /locus_tag="ACHKV2_002465"
     CDS             complement(55..786)
                     /gene="rlmB"
                     /locus_tag="ACHKV2_002465"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO6940337.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(967..3408)
                     /gene="rnr"
                     /locus_tag="ACHKV2_002466"
     CDS             complement(967..3408)
                     /gene="rnr"
                     /locus_tag="ACHKV2_002466"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO6940338.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKCEKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3447..3872)
                     /gene="nsrR"
                     /locus_tag="ACHKV2_002467"
     CDS             complement(3447..3872)
                     /gene="nsrR"
                     /locus_tag="ACHKV2_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO6940339.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4077..5375)
                     /gene="purA"
                     /locus_tag="ACHKV2_002468"
     CDS             complement(4077..5375)
                     /gene="purA"
                     /locus_tag="ACHKV2_002468"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO6940340.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5479..5676)
                     /gene="yjeT"
                     /locus_tag="ACHKV2_002469"
     CDS             complement(5479..5676)
                     /gene="yjeT"
                     /locus_tag="ACHKV2_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO6940341.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5758..6762)
                     /gene="hflC"
                     /locus_tag="ACHKV2_002470"
     CDS             complement(5758..6762)
                     /gene="hflC"
                     /locus_tag="ACHKV2_002470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO6940342.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6765..8024)
                     /gene="hflK"
                     /locus_tag="ACHKV2_002471"
     CDS             complement(6765..8024)
                     /gene="hflK"
                     /locus_tag="ACHKV2_002471"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO6940343.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8110..9390)
                     /gene="hflX"
                     /locus_tag="ACHKV2_002472"
     CDS             complement(8110..9390)
                     /gene="hflX"
                     /locus_tag="ACHKV2_002472"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO6940344.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9467..9775)
                     /gene="hfq"
                     /locus_tag="ACHKV2_002473"
     CDS             complement(9467..9775)
                     /gene="hfq"
                     /locus_tag="ACHKV2_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO6940345.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9861..10811)
                     /gene="miaA"
                     /locus_tag="ACHKV2_002474"
     CDS             complement(9861..10811)
                     /gene="miaA"
                     /locus_tag="ACHKV2_002474"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO6940346.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10804..12651)
                     /gene="mutL"
                     /locus_tag="ACHKV2_002475"
     CDS             complement(10804..12651)
                     /gene="mutL"
                     /locus_tag="ACHKV2_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO6940347.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12661..13998)
                     /gene="amiB"
                     /locus_tag="ACHKV2_002476"
     CDS             complement(12661..13998)
                     /gene="amiB"
                     /locus_tag="ACHKV2_002476"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO6940348.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14017..14478)
                     /gene="tsaE"
                     /locus_tag="ACHKV2_002477"
     CDS             complement(14017..14478)
                     /gene="tsaE"
                     /locus_tag="ACHKV2_002477"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO6940349.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14450..15997)
                     /gene="nnr"
                     /locus_tag="ACHKV2_002478"
     CDS             complement(14450..15997)
                     /gene="nnr"
                     /locus_tag="ACHKV2_002478"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO6940350.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15996..17135
                     /gene="queG"
                     /locus_tag="ACHKV2_002479"
     CDS             15996..17135
                     /gene="queG"
                     /locus_tag="ACHKV2_002479"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO6940351.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17118..17171)
                     /locus_tag="ACHKV2_002480"
     CDS             complement(17118..17171)
                     /locus_tag="ACHKV2_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940352.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17405..17480)
                     /locus_tag="ACHKV2_002481"
     tRNA            complement(17405..17480)
                     /locus_tag="ACHKV2_002481"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Gly,seq:gcc)
     gene            complement(17517..17592)
                     /locus_tag="ACHKV2_002482"
     tRNA            complement(17517..17592)
                     /locus_tag="ACHKV2_002482"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17557..17559),aa:Gly,seq:gcc)
     gene            complement(17803..18348)
                     /gene="orn"
                     /locus_tag="ACHKV2_002483"
     CDS             complement(17803..18348)
                     /gene="orn"
                     /locus_tag="ACHKV2_002483"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6940353.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18443..19495
                     /gene="rsgA"
                     /locus_tag="ACHKV2_002484"
     CDS             18443..19495
                     /gene="rsgA"
                     /locus_tag="ACHKV2_002484"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6940354.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19592..20560
                     /gene="asd"
                     /locus_tag="ACHKV2_002485"
     CDS             19592..20560
                     /gene="asd"
                     /locus_tag="ACHKV2_002485"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6940355.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20582..23905
                     /gene="mscM"
                     /locus_tag="ACHKV2_002486"
     CDS             20582..23905
                     /gene="mscM"
                     /locus_tag="ACHKV2_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6940356.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24056..25558)
                     /gene="yjeM"
                     /locus_tag="ACHKV2_002487"
     CDS             complement(24056..25558)
                     /gene="yjeM"
                     /locus_tag="ACHKV2_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6940357.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25777..26754)
                     /gene="epmA"
                     /locus_tag="ACHKV2_002488"
     CDS             complement(25777..26754)
                     /gene="epmA"
                     /locus_tag="ACHKV2_002488"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6940358.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27079..28887
                     /gene="frdA"
                     /locus_tag="ACHKV2_002489"
     CDS             27079..28887
                     /gene="frdA"
                     /locus_tag="ACHKV2_002489"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6940359.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            28880..29614
                     /gene="frdB"
                     /locus_tag="ACHKV2_002490"
     CDS             28880..29614
                     /gene="frdB"
                     /locus_tag="ACHKV2_002490"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6940360.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29625..30020
                     /gene="frdC"
                     /locus_tag="ACHKV2_002491"
     CDS             29625..30020
                     /gene="frdC"
                     /locus_tag="ACHKV2_002491"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6940361.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30031..30390
                     /gene="frdD"
                     /locus_tag="ACHKV2_002492"
     CDS             30031..30390
                     /gene="frdD"
                     /locus_tag="ACHKV2_002492"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6940362.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30453..31586
                     /gene="ampC"
                     /locus_tag="ACHKV2_002493"
     CDS             30453..31586
                     /gene="ampC"
                     /locus_tag="ACHKV2_002493"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6940363.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31674..32207
                     /gene="blc"
                     /locus_tag="ACHKV2_002494"
     CDS             31674..32207
                     /gene="blc"
                     /locus_tag="ACHKV2_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6940364.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32204..32521)
                     /gene="sugE"
                     /locus_tag="ACHKV2_002495"
     CDS             complement(32204..32521)
                     /gene="sugE"
                     /locus_tag="ACHKV2_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6940365.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32697..32843)
                     /gene="ecnB"
                     /locus_tag="ACHKV2_002496"
     CDS             complement(32697..32843)
                     /gene="ecnB"
                     /locus_tag="ACHKV2_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6940366.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(32954..33079)
                     /gene="ecnA"
                     /locus_tag="ACHKV2_002497"
     CDS             complement(32954..33079)
                     /gene="ecnA"
                     /locus_tag="ACHKV2_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6940367.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33131..33697)
                     /gene="efp"
                     /locus_tag="ACHKV2_002498"
     CDS             complement(33131..33697)
                     /gene="efp"
                     /locus_tag="ACHKV2_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6940368.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33739..34767
                     /gene="epmB"
                     /locus_tag="ACHKV2_002499"
     CDS             33739..34767
                     /gene="epmB"
                     /locus_tag="ACHKV2_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6940369.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8280 a         9572 c         8895 g         8154 t
ORIGIN      
        1 tatagacaaa aaaaaaccat ccaaatctgg atggcttttc ataattcaga ggaactagct
       61 gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt tcagggaaga
      121 aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg
      181 cataccttca ccttccgcgc ccatcaccag cgccaggcgt ccggtcattt tgctttgata
      241 gagcgtatga tccgcctcac ctgccgtacc gacgatccag atattctctt cctgcaacat
      301 acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg ccgcgccgca
      361 ggctactttt ttcgccgtgg cgttgagctg tgcggagcga tctttcggca caatcaccgc
      421 gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg gatcggttac
      481 accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg gcagatcgtt
      541 ttcctggtac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc
      601 gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct gggactcaag
      661 ggcgtggatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa agacttcctg
      721 aaaacgttca ggggcgcgct ccagcagggc ctgcactgca tggatgccgt aaatcatttc
      781 gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat ccggtctaca
      841 tgatccctgc aatatattga atttgcgtga ttatgtaggc cggataaggc gttcacgccg
      901 catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttctgt ttgaagaggg
      961 taatgatcac tctgccactt ttttcttcgc cgcacgcttc gctttggtcg ctgcagctat
     1021 tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg tctttcttcg ctgctttcgg
     1081 cttcgttttt ttctcaccgc ggaaggcgct gtctggctca aagtttacct ttttaccgac
     1141 ctgacgacgc ttgccgcctt ttttacctgc atcgcctttt ttcgctttct cgcgcgccgt
     1201 tttaccgacg ttgcgcggtg cgcgttcgct ggagatcagg ctaaagtcga ttttgcgctc
     1261 gtccatatta accgcttcga cgcgaacttc cacgcgatcg cccaggcgat aagtctggcc
     1321 gctggattca cccatcaggc gttgccctac ctggtcaaag cgatagtagt cattgtccag
     1381 cgaagagaca tggaccagcc catcaatgaa caagtcgtcc agacggacga agaagccaaa
     1441 gccagtgacg ctggaaatta cgcctttaaa gacgttacct acctggtcga gcatgaagtc
     1501 acacttcagc cagtcagaca catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga
     1561 acagtgctga cccaattgca gcatctcttc catcgaataa tggtagccac cggtttcagt
     1621 ggtgttgccc tgatgcccct gctctttcgc cagcagatac ttaatggcgc ggtgcagcat
     1681 caggtcagga taacgacgaa tcggcgaagt aaagtgcgca taggactgca atgccaggcc
     1741 aaagtgacca cggttttccg gatcgtaaat cgcctgtttc atcgagcgca gcagcatggt
     1801 ttgcagcatt tctgcatcag gacggtcggc aaccgactcc agcagctccg cgtagtcgcg
     1861 cggttccggc ttgttaccgc ccggcagttc cagccccagc tctgccagca ctgaacggaa
     1921 agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc
     1981 tttctcaacg aaacgcgccg ccgagatatt cgccagaatc atgcactctt caattaactt
     2041 gtgtgcgtcg ttacgctgag tctgttcgat acgttcaata cggcgttcag cgttgaaaat
     2101 gaacttcgct tcttcgctct caaatgagat cccgccgcgt tcttcacggg ctttatccag
     2161 cactttatag aggttatgca actcttcgag atgcttaacc agcggggcgt actgctcgcg
     2221 cagatcctga tcgccctgca gaatatgcca gactttggtg taggtcagac gcgcgtgaga
     2281 gctcatcacc gcttcgtaga atttgtagcc agtcaggcgg cctttcgacg aaaccgtcat
     2341 ctcgcacacc atacacaggc ggtctacctg cggattgagc gagcacagac cgttagagag
     2401 cacttccggc agcatcggga taacctgcga agggaagtac accgacgtac cacggttacg
     2461 cgcttctctg tccagcggcg ttggcggacg cacatagtag ctgacgtcag caatcgcgac
     2521 ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa accgcatcgt caaagtcacg
     2581 ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc aaatcgacac ggcccgcttt
     2641 tgcttcttcc ggcacttctt ctttcagccc agcaacctgt tgctcaacag cctgcggcca
     2701 gatgtacgga atttcatggg tacgcagagc gatatcaacc gccatgcccg tgcccatatt
     2761 gtcgcccagc acttcgacga ttttacccac cgctttggta cggcgagtcg ggcgttgggt
     2821 cagttcaaca accaccacaa agcccatccg cgcgcccatg atctgatcgg gcgggattaa
     2881 gatatcgaag ctcagacggc tatcgtcagg aaccacaaag ccgacgcccg cttcggtaaa
     2941 gtagcgacca acaatctggc tggtttttgg caccagtacg cggacaatac gcgcttcacg
     3001 acgaccttta cggtcagcac ccagcggctg agccagcacc tgatcgccat gaatgcaggt
     3061 tttcatctgc tcgctggaga gatacaaatc atctttacgc ccttcaaccc gcagaaagcc
     3121 gtagccatca cggtggccaa taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc
     3181 atagcactga cgacgagtaa agaccagttg accatcgcgc tccatcgcgc gcaggcggcg
     3241 acgcaggcct tcaagctgct cttcgccttc aatgtgcagt tctaccgcca gctcatcacg
     3301 gctggccggt ttttcacatt tggttaaatg ttcgaggata aattcccgac tagggatggg
     3361 attcgcgtat ttttcagctt cgcgttcctg gaaaggatct tgtgacatct cggttcctcc
     3421 gttgtcatct ctgatgaaga ttttcgtcac tccaccagca ataatttata aagcggttga
     3481 ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg
     3541 gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa tgtggcaaaa ctcactgctg
     3601 caattcacca gcgataaggg ctccagctcg cgcaccacat caccaatacg tatcgcactc
     3661 gccggtttac ccaggcgaat gccgccattt tttccacgaa cagcagtcac gtagccggca
     3721 cgactaagtt gattgattat tttgaccata tgattacggg agacgccgta gacgtcagtc
     3781 acttcagaaa tactggtcat ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt
     3841 aatccgtaat cagtgaaact cgttaactgc acatcaacct caaaagggaa atcgggaaaa
     3901 aatacattta tattgatgat aaaccagcca caagctgtgt cgctaattta tttcagttcg
     3961 gggaaggaaa aaagcgagga tttaacactg tgtcggatag cgtaatggct taaccgacac
     4021 agcaatgtat cacactgtta gcccggcagg caaaatatct gccaggcgta ccagaattac
     4081 gcgtcgaacg ggtcgcgcag aatcatggtt tcagtacgat ccggaccggt agagatgata
     4141 tcgatcggta caccggtcag ctcttcaatg cgcttgatgt agttcagcgc cgcctgcggc
     4201 aggccgctac gatctttcac gccgaaggtg gattcagacc agcccggcat ggtttcgtaa
     4261 atcggctcta cacctttcca gtcgtcagct gccagcggag tggtagtcac ttcacgacca
     4321 tccggcatac ggtaagccac acagagtttc acctctttca ggccatccag aacgtccagt
     4381 ttagtcaggc agaagccaga cagggagttc agctgtaccg cacgacgaac ggcaacggtg
     4441 tccagccagc cggtacgacg acgacgaccc gtagttgcgc cgaattcgtt accctgcttg
     4501 cagaggaact cgccagtttc atcaaacagt tcagtcggga acggacctgc acccacacga
     4561 gtggagtaag ctttgaggat acccagaacg taatcaacat aacgcgggcc caggccggaa
     4621 ccggtcgcca cgccaccagc agtggtgttg gaagaagtta cgtacggata agtaccgtgg
     4681 tcgatatcca gcagcgtacc ctgcgcacct tcgaacatga cgaaatcgcc acgctgacgc
     4741 gcctggtcga gcaggtcaga aacgtcaacc accatagaag tcaggatgtc ggcaacagcc
     4801 atcgtatcat ccagaacttt ctggtaatca accgcttcag ctttgtagta gttaaccaac
     4861 tggaagttgt gatattccat cacttctttc agtttttcag cgaaggtttc tttgtcgaaa
     4921 aggtcgccaa cacgcagacc gcgacgtgcc actttatctt cataagcagg cccgatacca
     4981 cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca
     5041 acatgataat caaggatcag cggacatgct tcagacagca gcagacgctc acgaacgggg
     5101 atgccacggt cttccagttc tttcatctct ttcatcagcg cggccggaga cagcacaaca
     5161 ccgttaccga tgatgctagt tacattctcg cggagaatac ctgatggaat aagatggaga
     5221 acggtttttt caccgttgat tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt
     5281 acaacatatt tagcccgttc agtcagaaga tcgacgatct tacctttacc ttcgtcaccc
     5341 cattgggtgc ccagtacgac gacgttgtta cccatttttc aaaatcaccg tttgcttaaa
     5401 aatggattct accatcgctt tttcagagtt acagcacttt ttgcactcaa aaatgacctc
     5461 gatcagtctg ttttttgttc agccaatcgt tttcctcaac atgtagtaga ccacaacgcc
     5521 cgcaaccaca agtccaccgc caaaacgacg taaaatatta tcgggcaaat tggtcatcgc
     5581 agagatcatc ttcttccatg ccttcgggta aagcatcggc cctaaacctt ccagtaccaa
     5641 aaccagggca agcgccagcc agattgtcga attcattcat tatccttata gaaaaagaaa
     5701 accaccgaca tccctgagga tgcggtggct ttattgacct gtaccgcagt cgttatatta
     5761 acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa tcgctatccg ggctcatgac
     5821 catcacgtcc tgattgccag agaagctgtt ctcataagca cgcaggctac ggatgaatgc
     5881 gtagaagtcc ggatctttac tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc
     5941 ttcaccgcgc atgatgcggc cctgacgctc agcttctgcc agcgttctgg tcacttcata
     6001 gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc
     6061 taccgcttca cgctcggcgc gcatacggtt gtagatcgct tcagacactt cggtcggcag
     6121 gttgatctgc ttaattcgca catcgacaac ttcaataccc agcgccgcca tactgttcgg
     6181 gttgatgacc ggaactttgc ccttcgtctc agccgttacg cgctctgccg cttcggcaat
     6241 ggcgttatct gccgccgggg tagtaacttc atcttctgta cccgcagaac cggagttcag
     6301 cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg gtgacgatgt ctttcacgtc
     6361 caggcgacca atttcagaac gcagacggtc agagaactta cgtttcaaca gcacttccgc
     6421 ttgcgaaatg tcgccccccc ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca
     6481 tttgatgtaa gagtcgacga tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg
     6541 gttgtccatg gtctgaatac gtgcgtcgag cattttcacc gtttcaatga acggtatctt
     6601 gaaatgcaga cccggctcat aaaccagagg tttgttgtca tcgtcacgca gtaccttacc
     6661 aaaacgcagc gtaataccgc gctcaccttc tttgacgaca aagacagaca tgtaaagcac
     6721 taccagcacg atgatgataa tcgcgataac tgacttacgc atcgttattc cccctgacgc
     6781 tggtagtcgt tacgctgcgc attggcgcgg cgttggtcca taatatcgcc ctgactggtg
     6841 gacgacgtgt tgcttgctcc actggttgtg gaggaagagg ctggcggcag acgcagcaga
     6901 ttgctggcac cgttatcgct cttcgccgca ggggcgttac cacctttcag catctggtct
     6961 aacggcagaa ccatcaggtt gccaccttta tcgttaacca gcactttgcg ggtgttaccc
     7021 aacacttttt ccatcgtctc gatatacaga cgctcgcgag taatttccgg cgcggcttta
     7081 tattccggca gaagtttagc aaaacgcgcc acttcaccct gagcttccag gatggtctgg
     7141 gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct gaccgttcgc acgcggctga
     7201 acttcgttgg tatacgcttc tgcttcacga atgtattgct gttcgttttc acgcgcggca
     7261 atcgcatcgt caaacgccgc ttttacttct tccggcggac gagcagcctg gaagttgacg
     7321 tccagcagcg tgatacccat gtcatacgga cgaatcgtct cttccagttc gcgctgagta
     7381 tcgctacgaa tcacggtacg accttccgtc agaatgcggt ccatggtgta tttaccgata
     7441 actccacgca gggcgctgtc ggtagcctga cgcaggctgt catccgggct ggtcacgcta
     7501 tacagatatt tttccggatt ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg
     7561 ttctcgtccg acgtcagcat cacaccagaa gctgccagtt cacgcacggc ttccacgttc
     7621 accggtttga cttcgtcgat aaacgtcggt ttccagttca gacctggctc aaccagatgg
     7681 ctgaatttac caaagcgtgt taccacgccg cgttcggctt ctttaatggt atagaaacca
     7741 ctggccgccc agataatgac aatcgctgcc gctgcgatgg taacgacacg accgccaagc
     7801 tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc cggtgccttt accgccgccc
     7861 agaccaccga gctttttgct cagtttgcgg aagatatcat ctaaatcagg tggcccttga
     7921 tcgcgaccgc ctttgtttcc atttccctca gagttgccgc caggtttgct gcttccccac
     7981 gggtcgcggt cttgtccgtt attaccgggc tgattccacg ccatgtttgt gctccatatt
     8041 tgttatgcgg tgatccctgt tgtctttcga cgccagagga tatgactcca cgcttcagac
     8101 gctacgccgt tagatcaggt aatcgatcaa cgccggttct tgtttacaga ggcgacgcca
     8161 gtcaacgatc ggcatacgaa cttgcagact tacgctgccg tcctcctcca tccactcttt
     8221 ttctattgcc tgaagctgat aaaaacgact tctcagacgc ccttcctgcg gtggcagacg
     8281 caatgtatgc tgcgccacct cgccggaaag ccgctccgtc aaagcctgaa aaagctgtgg
     8341 tatccccgct ccggtctgtg cggaaagcca gacacggatc ggtttgttct cttcgtcccg
     8401 atcaatacgc ggttcgaaat cttccagcat atcgatcttg ttcatcacca gcagggttgg
     8461 gatctcgtga gcgtcgatct cttcaagaac cgtattcacc gcttcgatgt tttcttgtac
     8521 acgcacatcc gccgcatcaa tcacatgcag cagcaatgtg gcttgccgcg tctcttgtaa
     8581 cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc
     8641 tgcaagtacg gtttcaccga catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt
     8701 ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg atgcgattga aaagggtaga
     8761 tttaccggcg ttggtatatc ccaccagcga aacagtagga acgtcggctt tgatacgcga
     8821 ttgccgcccc tgctcacgct gcttttcaac tctttccagg cgcgactgta tctgcacgat
     8881 gcgattacgc aacaaacgac ggtcggtttc gagctgggtt tcacccggac cacgcaaacc
     8941 aatcccgcct ttctgtcttt caaggtgggt ccagccacgc accaggcgtg tagccagatg
     9001 gcgcagctgc gccagctcaa cctgcaactt accctcatgg gtacgcgcac gttgggcgaa
     9061 aatatctaaa ataaggccgg tgcggtcgat aacacgacac tcgcacaaac gctccaggtt
     9121 acgctcttgc gccgggctca gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt
     9181 gacagcttcc gcaatttcaa ctgctttacc ttcacctaca aaatactttg ggtgcggcgc
     9241 tttacggcta ccggtaatca cctgcaatgc ttcgacaccg gcggaagaga ccagagattc
     9301 aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc
     9361 ctgctcacca gcatcataac ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg
     9421 aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc gaaaccttat tcggtttctt
     9481 cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc atgatggtag ttactgctgg
     9541 taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg agacgggaca acagtagaaa
     9601 tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa caggatcacg aactgatcaa
     9661 aagactcgat ttgcccttgc agcttaatac cattcaccaa ataaatagaa actggaacac
     9721 gttcccgacg cagtgcgttc aggaacggat cttgtaaaga ttgcccctta gccattctct
     9781 cttttcctta tatgcttatt tgtactttga acctttcgat tctgaaaaaa ttgcgcacga
     9841 tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa caacctgtaa tacttcgtca
     9901 cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc cttcccaacc acgcagccag
     9961 gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac ctcgataaac catttcatcg
    10021 tatgagattt cgcctccaag gtaagaccac atctggcgat aacccacgca acgaatggaa
    10081 ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc ggacttctgc ttcaaaacct
    10141 gaagccaaca tctgatgaaa acgctgctca attcgttgat ggagcagttc acggctcgcc
    10201 ggggcgatgg cgaactgatg cacctgatac ggtagagcgt ctcctgacgt ttgcgtcagt
    10261 tccgttaaag ttttacccga aatgaaaaaa acttccagtg cccgggaaag cctttgtgga
    10321 tcatttggat gaatccttgc tgccgcaacc ggatcgatct cctgaagttg acgatgcaat
    10381 gactcccaac cttgctctgc cgcctgttgc tcaattctgg cccgtacttc cgggtctgcc
    10441 gacggtagcg gcgacaaccc ttccagcaat gccttgaaat acaacatcgt accgcccact
    10501 aacagtggga tccgccccgc cgcggtgata tcggccattt ccgccagcgc atcgcggcga
    10561 aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat ccagcaatcg gtgcggcgcg
    10621 gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat ccatcccttt gtaaataagg
    10681 gcagaatcaa cgcttatcaa ctctactggt aaaattttac gcagctcaat ggctaacgcc
    10741 gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct taggcaggct cgccttactg
    10801 atatcactca tctttcaggg cttttatcgc cggatgtaaa tcaacagatt gtaacagacc
    10861 acccggcggc gttttcacaa gttgcggaca taaccgttcc acgtccgcca gcagggttat
    10921 ggcctgtgcc attgaccact gcgcatgttc gctcatcaga tttcgtgcaa tccactgcgc
    10981 aatattgcca ggttcgaata cggactgctt cgctaggtag cctatcagtt caggaatcaa
    11041 gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc ctgatggtca catgctgtgc
    11101 atctgactgg aaatcaatac ccaattccgc cagggcagac tgcgcttttt ctaatgccga
    11161 tttttcttcg ccagaaactt ttagccgcaa cggaatcagc aacggctggg cgcaaacggg
    11221 cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt tctgccactg gcaaggctaa
    11281 aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg gaatggacga tagtcagtac
    11341 ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc tgcggttccg gcgcttttgg
    11401 tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac acttcacctt gctgtttctg
    11461 gtaacctggc tgcgcattcg gccagggggc agccggacga ctacctgatg ctggcgcagg
    11521 agtgtagcgc ggagctactg gctcacgaac tgccggttca gcaaagtgat tgcgccccgc
    11581 cgccacgcgg ttttccggaa tgggacgcgg tgcaggttgg ggttcatcgt ccagcggtag
    11641 cggcgtttcc agttgctgtt gtagcacgct cagcacgccc tgatagataa agtcatgcac
    11701 cagacgcgac tgatgaaaac gcacttcgtg tttggcgggg tgcacgttga cgtccacctg
    11761 atgcgggtcg atctccagat acaacacaaa tgcaggttgc tgatcggccc ccagtttgtc
    11821 ttcacaggcc tggcggatcg cgtgattgat caggcgatcg cgcatcatgc gaccattcac
    11881 gtagcaatac tgaatttccg ccagtgcggg cgtggtgtga tttggatcgg ccacccagcc
    11941 gcgcagcgtg agatcgccgt gttgccattc aatcgccagc gcttgttcga gaaaagcggt
    12001 gccacaaatc gcgcctaagc gccgttcttt ttgcccgcct tccggcaccg cgcggtactg
    12061 gcgtacgatt ttgccgttat gcgacaggtt gatcgtgacg tcgaaacgcg ccagtgcgat
    12121 gcggcggatg atttcatcga tatggttaaa ttcggttttc tcggtgcgca ggaatttgcg
    12181 ccgcgccggg gtgttgtaga acaggtccag tacctccagc gtcgttccca ccggatgcgc
    12241 cgccggttta accgtcacgt ccatatcgcg cccttcggca taggcctgcc aggcttcctg
    12301 ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc gaactgatac tcgccagcgc
    12361 ctcaccgcga aagcccaggc tgataatggc ttcgagatcg tccagagagg cgattttact
    12421 ggtggcatga cgcgccagcg ccagcgccag ctcgtctttt ttgataccac agccgttatc
    12481 acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca atatcgatac gcgtcgcacc
    12541 tgcatcgagg ctgttttcca ccagttcttt gactaccgac gcaggtcgct cgaccacctc
    12601 acctgcggca atctggttcg ccagttgtgg cggtaagacc tgaattggca tcaatcgtcc
    12661 ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg gcagtttgtg ccgttgcacc
    12721 ctgcggcgca gattgcatcg gatgcgcaag gaaataattg cgcaggcctt tgtaaatggc
    12781 ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa cgttcttcgc tgttgttgct
    12841 gataaaaccg gtttcgacca gtactgatgg gatatccggc gagcgcagaa cgccaaggct
    12901 ggcgtgttct ggtcgacgtt tatgtatttc gccaatgcgt tgcaactgac tgatcatact
    12961 ggtcgctaca tcatacccta cccgctggga atgaccgaac tgtaaatcca gcaccgcctg
    13021 gcttaaatag gggtcagact gactgttcgc cagcacatca cccgccccgc ccagcagctc
    13081 cgactgtttc tcgtgctgct ccagccagct ggccatttcg ctgttggcgc gacggttaga
    13141 gagcacccat acggaagcgc cagtcgcact gcggttcggt gcggcatcag cgtgaatcga
    13201 cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg cgccccatca ccgagataaa
    13261 gtaatccccg tcacgggtta aaacgccttt aaacatcgga tcgtcattga gcaaagtacg
    13321 caatttacgc gcgatggcga tggtgacatt tttctcccgc gtaccaccgg ggccgatagc
    13381 gccagggtcc tgaccgccgt gtccggcatc aatagcgatg ataattttat cgccattgtt
    13441 agccgtcgcg cgcgctgccg gacgcgttac cgtattactg ctgataacac ccgtagtgcg
    13501 gttactttcc gttttaaacg gattgcgcgc cggttcgctg acgcgcggtg cgacaaccgt
    13561 aggcgtttca acgcgtttca caaccacagg cggcggagga ggcggtggcg gcgcatcggc
    13621 gttaatcgta aagacgacag tgtaattgct gccattctgc cgcttcaccg cttcggtttt
    13681 accattttcg gtaagatcga ccaccagccg tagcgtttgt gcatctttag gcgttccgga
    13741 gcgaatcgcc ttcaccagat tattaccgct gaacaacaac ggcagtccct gaatcacgcc
    13801 cgtttgtttg atatcgagcg ccacggtgcg tttgctttga tggctaaacg cataatcagg
    13861 atcgccaata aaactcaacg ttatccgcgc ctgttggttg ccgttagaaa cctgaatatc
    13921 agagagcgtc gcggcaccca cctgcgcgca cagcagcagc aacgtcgcta ccaaccaatt
    13981 tctgatgcga tacatcatcc caccaccttt caaaggttaa ccggctaaac gtgccagcaa
    14041 caattcaccc gcagaggaaa ccgcactcac gcgcgcctca cggccttgtg cctgataatc
    14101 aatgtgtatt tcgacatccg ggtcaggaag aacacctgta ccttgttgtg gccactccac
    14161 caggcagatg gcatcgttgg caaaataatc gcggatcccc ataaactcca gctcctcggg
    14221 atcggcaagg cggtacaaat caaagtgata aaccattaaa ttgtcgagcg tatagggttc
    14281 gaccagcgta taagtggggc ttttgacatt accctgatgg cccaaagcct gtaaaaagcc
    14341 ccggctaaag gtggttttac ctgcgcctaa atcgccatac agatagatta cggttgcgcc
    14401 atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt gcctgctcat cagggagcgg
    14461 aattactcga ttcatcatgg tttttatcag tcacttccgg gttaacaata cgctgtagcg
    14521 tggaaaagag atcggttgcc agcatcccgc gcgttccaaa acgcgccgcc agtacgtcag
    14581 ctgccgcacc gtgcgcgaca cagcctgcac aggctgcatc atacggcgac agtttttgcc
    14641 caagcaatgc gccaataata ccagagagca catcgcccat gccgccgctt gccatgcctg
    14701 catttccggc atcaataatg cctaaagcgt caggatgggc ggcgaccacg gttccggcac
    14761 ctttcagcac cgctacgccg ccataacgtt gtaccagacg tttggcgcaa tgtaagcggt
    14821 cactttcaat ttcagcgacg gaacagccta acaaccgtgc ggcctcgcca ggatgcggcg
    14881 tgatcacgcg attgtgacgc ttatcgggat tgattgccag caggttcaat gcatcggcat
    14941 cccacaacat cggtttgcga aaattctcaa ctttttgcag ggcttttttc ccccactctt
    15001 gctggcccag accgggacca atcaccacca catcggccca ttccaggctt tcggtaagag
    15061 agtccatagt cagttcatgc accatcaatt ctggtcgtgc agtcagcagc ggcgcaatgt
    15121 tctcactgcg ggtcagtact cggactaaac cagcaccagc acgtagcgcc gcttcccccg
    15181 tcatacgaat agcccccgcc gtgccgtgat cgccaccgat aatcaccagc cgcccgtgat
    15241 cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg agaaagttgt tctgccgaaa
    15301 accgctgaat tttcgtctcc tgacctgcca gccagctatc cagccccagt gagtcaaaat
    15361 gcagttgtcc tgtaacatcc cgcgcttttc cagtgagcaa gcctggtttc agcgcaataa
    15421 aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt agcgccggtt tcagccagca
    15481 ggccggaagg gatatcaacc gccacaatcg gcgcaggatg ggaattagcg tggtcgatta
    15541 actggctaat ggattcgcgg ggtgcttgct gcaagccggt accgagcagc gcatcaacaa
    15601 tcagagctac cgactcgggc cagacaatat tcgaagcatg gatctcgcct cccgcgttta
    15661 accatgcctc gcgtgccagc gcggcctctt ccggcaacgg tttatcgctc tcctgggcca
    15721 acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc gaccacgtag ccatcgccgc
    15781 cgttattacc atgaccgcac aacaccagcc agtggcgggc gtcaggatac gccgaacgac
    15841 acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc atagagtgtg agccccagcg
    15901 catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc gtgccagacg gtgtgtggta
    15961 tacttacggg gtttttcttc attgtatggt ccgtcatgtc agagcccctc gatctcaatc
    16021 agttagcgca aaaaattaaa cagtgggggc tggaactggg ctttcagcag gtaggtatta
    16081 ccgataccga tctcagcgag tccgagccca aactgcaagc atggctggac aaacaatacc
    16141 acggcgaaat ggactggatg gcacgtcacg gtatgctgcg cgctcgcccc catgagttat
    16201 tgcccggtac gctgcgcgtg atcagcgtgc ggatgaatta ccttcctgct aacgccgcat
    16261 ttgccagcac gctgaaaaac cccaaactcg gctatgttag ccgttatgcg ctgggccgtg
    16321 actatcacaa acttctgcgc aaccgactca aaaagctggg cgagatgatt cagcaacatt
    16381 gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc tattctcgag cgcccgttag
    16441 ctgaaaaagc tgggctcggc tggacaggta agcactcact tatcctcaat cgcgaggccg
    16501 gttcgttctt ctttttaggc gaattgctgg tcgatattcc gctgcccgtg gatcaaccag
    16561 tcgaggaagg atgcggcaaa tgcgtggcct gtatgacgat ttgcccgacc ggtgccatcg
    16621 tcgagccata taccgtcgat gctcgccgct gtatctctta tctcaccatc gaactggaag
    16681 gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg tatttacggt tgcgatgact
    16741 gccagcttat ctgcccgtgg aatcgctatt cacaactcac tacagaagac gatttcagcc
    16801 cgcgtaagcc gctacacgca ccggaactca ttgagttatt cgcctggagc gaagagaagt
    16861 ttttaaaagt cacggaaggt tcggcgattc gccgtatagg tcacctgcgc tggctgcgta
    16921 atatcgccgt ggcattaggc aatgcgccct gggatgaaac gattctggcg gcgctcgaga
    16981 gtcgcaaagg tgagcaccca cttcttgatg agcacatagc gtgggcgatg gcgcagcaaa
    17041 tcgagaggcg aaatgcgtgc atagtcgaag tgcaattgcc gaaaaaacag cgtctggttc
    17101 gggtaattga aaaagggtta ccgcgtgacg cctgattcat ccacagcttg tgaataaaaa
    17161 taaaaacgca ttgcaattca agacgaataa aatcgtcaag tgatcgactc gacaaaacga
    17221 aatcttaatt tttaaattat ctatcaatag gttaaaaaaa tactattcac cgtgcgaagt
    17281 ttctttgaaa ttaagattac ggcctgaggc tgtggataac tctgtttaca agagtttgtc
    17341 agagacaaca aaaaccagca ataacgcatc gcgtcgctgt ggatatttta ttgagagaag
    17401 aatttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17461 accaactgag ctattcccgc ttgggtggtc tgtgctttgc agcacttttc aaattttgga
    17521 gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg
    17581 agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct gcgtcgttac
    17641 gggaggcgca ttatacgaga aatctttccc tctgcaaccc ccctaaaaat ttttttttga
    17701 gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc aaaatcacgc
    17761 cgccgcaagt ggattccggc gcttacgtga ccagcaaaaa ttttacagct tgataaaatg
    17821 ctcgcggtag taagccagct ccgccaccga ttcacggata tcatccatcg cctgatgcgt
    17881 cccctgtttg gtaaaaccat caagaatttc cggcttccag cggcgcgcca gctctttcag
    17941 ggtgctgaca tcgagataac ggtagtggaa gtaggcttcc agctccggca tgtatttaaa
    18001 caggaagcga cgatcctgac cgatgctgtt accgcaaatc ggcgatttac ccgcaggcac
    18061 ccactgtttt aaaaattcga gcgttgccag ttcagcttca cgatcgccca tcgtgctcgc
    18121 tttcacgcgc tccaccagcc cgctggcggt atgggtgcgc acgttccagc catccatcag
    18181 cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca gaatattcag
    18241 attggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg gatccagacc
    18301 ggtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc tattttccac
    18361 ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt tgcccatcag
    18421 gggcgaccag gagtcagtac gattgagtaa aaataaactc tccaaaggcc agcagcgccg
    18481 cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg actacgacga
    18541 caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta tgcacgctga
    18601 tgtggaatcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca tccgttcgct
    18661 ggtgaccggc gaccgcgtag tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt
    18721 caaagggatc gtggaagcgg tgcatgagcg cacctcagtg ttgacgcgtc cggatttcta
    18781 cgacggcgtg aaacctattg ccgccaacat cgaccagatt gtcattgtct ccgccatttt
    18841 gccggagctg tcgctcaata ttatcgaccg ttacctggtg gcctgcgaaa ccttgcagat
    18901 tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca tggcgttcgt
    18961 caacgaacag atggatatct accgcaatat cggttatcgc gtattgatgg tttccagcca
    19021 tactcaggat gggctaaaac cgctggaaga ggcgttgacc gggcgcatca gcatttttgc
    19081 cgggcagtct ggcgtcggca aatccagcct gctgaacgcg ttgctggggc tgcaaaaaga
    19141 gatcctgacc aacgatgtct ctgacaactc ggggcttggt cagcacacca cgaccgccgc
    19201 acgcctgtat cacttcccgc acggcggtga tgtgattgat tcccctggtg tgcgtgagtt
    19261 cggcctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat tccatgacta
    19321 tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct gcgctatccg
    19381 ggaagcggtt gaggaaggaa aaatcgcgga aacccgtttc gaaaactatc accgtattct
    19441 ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg actgacaatt
    19501 aagctaaccg tcgttagaat cgtccccttt ttttcaggat ccggcattta ggccggatca
    19561 ggaacgacaa aacaatggcc tggaggctac cttgttaaat tcatttaaac tttcgctaca
    19621 gtacattctg ccgaaactat ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc
    19681 aggatggctg acaaaactgg ttatcgatct gttcgttaaa tactacaagg tcgacatgaa
    19741 agaggcgcaa aagccggaca ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc
    19801 gctgcgtgac gaagtacgcc caatcgatac cgatccgaat gtcctggtca tgcctgccga
    19861 tggcgttatc agccagttgg gtaaaatcga agaagataaa atcctgcaag ccaaaggcca
    19921 caactacagc ctcgaagccc tgctggcagg caactatctg atggcggacc tgttccgcaa
    19981 cggtacgttt gtgaccactt acctctcccc gcgtgactac caccgcgtac acatgccgtg
    20041 caacggtatt ctgcgtgaga tgatctacgt gccgggcgat ctcttctccg ttaaccatct
    20101 cacggctcag aacgtgccga atctgtttgc ccgtaacgaa cgcgtgatct gcctgttcga
    20161 taccgaattt ggcccaatgg cgcagattct ggtcggggcg acgattgtag gcagcattga
    20221 gacggtctgg gcgggcacca ttacgccgcc gcgcgaaggc atcatcaagc gctggacctg
    20281 gcctgccggg gaaaacgacg gttctgtggc gctgctgaaa ggccaggaaa tgggtcgctt
    20341 taaacttggc tctaccgtta tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca
    20401 actggaaagc ctgtctgtca cgaaaattgg ccagccgctg gcagtatcta ccgaaacctt
    20461 tgttacgcca gacgctgaac ccgccccgct tcctgctgaa gagatcgaag cagaacacga
    20521 cgccagccca ttggttgacg ataaaaaaga ccaggtctaa tccatcaaag gaaacgctga
    20581 cgtgcgcctg attatcactt ttctgatggc ctggtgcctc agttgggggg cgtacgccgc
    20641 gacggccccc gatagcaaac aaatcaccca ggaactggag caggcaaaag cggcgaaacc
    20701 cgcgcagcca gaagtcgtag aggcactcca gtctgcctta aatgcgcttg aggaacgaaa
    20761 aggttccctt gagcgcatca agcaatatca agaagtcatt gataattatc cgaaactctc
    20821 cgctactctg cgcgcacaat taaacaacat gcgtgacgag ccgcgcagcg tgtcgccggg
    20881 gatgtctacc gacgcgctga atcaggaaat tctccaggtc agcagtcagt tgctggataa
    20941 aagccgtcag gcccagcaag agcaggagcg cgcccgcgag attgccgatt cgctgaatca
    21001 actgccgcaa cagcaaaccg acgcccgccg tcagttaaat gagatcgagc gccgcctggg
    21061 gacgcttacc ggcaatactc cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc
    21121 tgcacgtctt aaggcgctcg ttgatgaact ggagctggcg cagctgtctg ccaataaccg
    21181 ccaggaatta gcgcgcttgc gctctgagct ggctgaaaaa gagagccagc aactggatgc
    21241 gtatttgcag gccttgcgta atcaattgaa cagccaacgc cagcttgagg cagagcgggc
    21301 gctggaaagt accgaattac tagcagaaaa cagtgccgat ttgccgaaag atatcgtcgc
    21361 gcaattcaaa attaaccgcg aactatcggc ggcgctgaat caacaggcgc agcggatgga
    21421 tctcgttgcc tcgcaacagc gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt
    21481 gaatacgctg cgtgaacagt cgcaatggct gggatcgtcc aatctgctcg gtgaagcgtt
    21541 gcgggcgcag gtggcacggc tgccggaaat gccgaaacca caacagcttg ataccgaaat
    21601 ggcgcagttg cgtgtgcaac ggttacgtta tgaggatctg cttaataaac agccgctgct
    21661 acggcaaatt catcaggccg acggtcagcc gctgaccgct gagcaaaacc gtattctgga
    21721 agcacagcta cgcactcagc gtgagttgct gaactcattg ttgcagggtg gcgatacgct
    21781 actgctggaa ctgaccaagc tgaaagtctc caacgggcaa ctggaagatg cgctgaaaga
    21841 agtgaacgaa gcgacccacc gctatctgtt ctggacctct gacgtgcgcc cgatgaccat
    21901 cgcctggccg ctggaaatcg cccaggatct gcgtcgtctc atttcgctgg acaccttcag
    21961 tcagttgggc aaagccagtg tgatgatgct gaccagcaaa gagacgattt tgccgctgtt
    22021 tggcgcgttg attctggtcg gttgcagtat ttactcgcgc cgctatttca cccgttttct
    22081 tgaacgttcg gcggcgaaag tcggcaaagt gactcaggat cacttctggc tgacgttgcg
    22141 cactcttttc tggtcgattc tcgtcgcgtc accgctgccg gtgctgtgga tgacgctggg
    22201 ttacggcttg cgcgaggcgt ggccttatcc gctggcggtc gcgattggtg atggcgtcac
    22261 agccaccgtg ccgctgctgt gggtagtgat gatttgcgct acctttgccc gcccgaacgg
    22321 cttgtttatc gctcattttg gctggccgcg cgaacgtgtt tctcgtggga tgcgctacta
    22381 cctgatgagc atcgggctta ttgtgccact gattatggcg ctgatgatgt tcgataacct
    22441 cgacgaccgc gaattttccg gttcgctggg acggctttgc tttatcctca tttgcggtgc
    22501 gctggcggtg gtcaccctca gcctgaaaaa ggccgggatc ccgctgtatc tcaacaaaga
    22561 aggcagcggc gacaatatta ccaaccatat gttgtggaac atgatgattg gcgcgccact
    22621 ggttgccatt ctggcgtcgg cggtgggtta tctggcaacg gcacaggcgc tgttagcgag
    22681 gcttgaaacc tcggttgcca tctggttcct gctactggtg gtttatcacg ttatccgccg
    22741 ctggatgctg atccaacgac gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga
    22801 gatgttagcg caacgtgcgc gtggcgaaga ggaagcacat catcacagta gcccggaagg
    22861 ggcaattgaa gtcgatgaaa gcgaagtcga tctcgatgcc atcagtgcgc agtccttgcg
    22921 gctagtgcgc tcaattttga tgttgatcgc cctgctttcg gtcattgtgc tgtggtcaga
    22981 aatccattcc gctttcggct tcctcgaaaa tatttcgctg tgggatgtca cctccacggt
    23041 acagggcgta gaaagtctgg agccgattac cctcggtgcg gtgctgattg ccattctggt
    23101 gtttatcatc accacgcagc tggtgcgcaa tctgcccgcg ctgctggaac tggcgatttt
    23161 gcagcacctg gatttaacgc cgggtaccgg ctacgccatc accaccatca ccaaatatct
    23221 gctgatgctg attggcgggc tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt
    23281 gcagtggctg gtcgccgcgc tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc
    23341 caactttatc tctggcctga tcatcctgtt cgaaaaaccg attcgcattg gcgatacggt
    23401 gacaattcgc gatcttactg gtagcgtgac gaaaattaat acccgcgcaa ccaccatcag
    23461 cgactgggac cgtaaagaga taatcgtgcc gaacaaggcg tttattaccg agcagtttat
    23521 caactggtcg ctctctgact cggtcacgcg cgtggtgttg acgattccgg cccctgccga
    23581 tgccaacagt gaagaagtga cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt
    23641 gatcgacaac ccggcaccgg aagtcttcct ggtggatctg caacagggga ttcagatttt
    23701 cgagctgcgt atttacgccg ctgagatggg tcaccgtatg ccgctacgcc atgagatcca
    23761 ccagctgatt ctggctggct tccatgccca cggtatcgat atgccattcc cgcccttcca
    23821 gatgcgtctg gaaagcctca acggtaaaca aacggggaga acgctgacgt ctgcgggcaa
    23881 aggtcgtcag gcgggaagtt tgtaatgcat gaagctgcca aatgatgtca acgtggctca
    23941 tttagcagct taacatcatc ttcagttatc accggccctg gagagtatca tccatacgca
    24001 ttacttcctg acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt
    24061 cgagagctaa ctcaggttta ttcgccatgc gactgcaata gttctggtaa atcgccatcg
    24121 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    24181 cttcaaccac aggctgaata atggtgaaga cgttcgcaaa tgtcaccacc agaacgacaa
    24241 ccaccgtcgc aatcattgcc gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat
    24301 cctgacgtgc tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag
    24361 acacgttcgc catcagcgtc agcttgttaa agaacgccga tgcggtaccg ccaccaaacg
    24421 aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatagcg atagacggca
    24481 tccccatcgc attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga
    24541 tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca
    24601 tcgaaagtcc ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca
    24661 aatgcagtgc gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc
    24721 cgaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgacg ccccataaaa
    24781 atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa
    24841 agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca attccgccat
    24901 aggcaaaaat agcaaatacc acaaacgata gcattgccag accggactga taacctgggt
    24961 tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag
    25021 taatgcttac taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag
    25081 taatgcgggc aattttatta atcccctttg aagcaacgac ggtgaccaga atcatccatg
    25141 ccactgccag cagaccaacc acctgcgtag gctccagtcc ggcaatacgc cagtgctggg
    25201 tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg gaggtgctca
    25261 ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc
    25321 cgacactatt attcatccag gaatagatac cgccttcttc tttgcgataa gcagctccca
    25381 tttcagccat cattaaggcg aatggaataa agaataacaa tgcagaaaat atataaaagg
    25441 gaatcgcact ataacccatt aagtaataag ccgatgggct attggcaaat ccaaatacgg
    25501 aagtaaagat catcaatatg agtcctatca gactcatctt ttttatcgtg tgaggcatga
    25561 aaccatcctt catttcagcg cgtgtataaa acgcgcccac tgaactgcac tgcccaacat
    25621 tgagcgggca gcgtaaaaac caatacagaa ttgatacctg gatgatatca aatatccagt
    25681 cttaaattgt ggcctttagc ggaaaaatga aaacatatgc tacagaatgg cgcttatcac
    25741 gccattcttc gctgttaatt cagtaatttt tcagaattat gcccggtcaa cgctaaaggc
    25801 gatgacttca gccagtgtct ccgcgcccag cgccaacatc accagacgat caacacctaa
    25861 tgccacgccg gaacagtcag gcataccgac tttcaaggct tcaatcagat tctgatcaat
    25921 ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta tcttgttcaa agcgttgttg
    25981 ctgctcacgg gcatccgtca attcatggaa accattcgcc agctcaatac ctttataata
    26041 aacctcaaag cgttcagcga cccgatgatc ttcggtactg atttgcgcca gtgatgcctg
    26101 gctggctgga aagtggtaca caaaggtcgg tttttcttta ccaatatttg gttctacgcc
    26161 aaaggtaaac agcaattgta gcaacgtgtc gcggtcttct tcggtatcag caacattgct
    26221 caaatccagt ttcgctgcca cttcccgcag ttgcgttttg tcggcagaga gcgggtcaat
    26281 ttccagataa cgcaagaaag cttgttgata agaaaggctt tctgctgccg ggcagtccag
    26341 cacctgttgt aagagatcgt ccacctcgtt catcaaccgg tacatatcat agtgcggtcg
    26401 ataccactca agcatagtga actcagggtt gtgataacgc cccatctctt cattacggaa
    26461 actgcggcac agctggaata ccggcccgca accggcaacc agcaggcgtt tcatatggta
    26521 ttccgggctg gtcattaacc agagattcat cccctgcgaa tgcccggggc caacgaaacg
    26581 tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc tggctcatac aaggcgtctc
    26641 cacctccagc actccacgat cggcaaagaa acgacggatc tccgccataa tcgccgcgcg
    26701 ttttaataag ttaggaatgg atgcgctcgg ctgccaggat gccgtttcgc tcatagttaa
    26761 atctccagtt tttgacaagg gcacgaagtc tactcgcaac gcgacggcga gacaaatttt
    26821 acgcaggaat aaaacaacgg tgggcagtga ctaaaaaaag cacgatctga tggtttagta
    26881 attaaattaa tcatcttcag tgataattta gccctcttgc gcactaaaaa aatcgatctc
    26941 gtcaaatttc agacttatcc atcagactat actgttgtac ctataaagga gcagtggaat
    27001 agcgttcgca gaccgtaact ttcaggcact taccctgaag tacggggctg tgggataaaa
    27061 acaatctgga ggaatgtcgt gcaaaccttt caagccgatc ttgccattgt aggcgccggt
    27121 ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa atccaaatgc aaaaatcgca
    27181 ctaatctcaa aagtataccc gatgcgtagc cataccgttg ctgcagaagg gggctccgcc
    27241 gctgtcgcgc aggatcatga cagcttcgaa tatcactttc acgatacagt agcgggtggc
    27301 gactggttgt gtgagcagga tgtcgtggat tatttcgtcc accactgccc aaccgaaatg
    27361 acccaactgg aactgtgggg gtgcccatgg agccgtcgcc cggatggtag cgtcaacgta
    27421 cgtcgcttcg gcggcatgaa aatcgagcgt acctggttcg ccgccgataa gaccggcttc
    27481 catatgctgc acacgctgtt ccagacctct ctgcaattcc cgcagatcca gcgttttgac
    27541 gaacatttcg tgctggatat tctggttgat gatggtcatg ttcgcggcct ggtagcaatg
    27601 aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg cggtcgttat ggctactggc
    27661 ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca tcgttaccgg tgacggtatg
    27721 ggtatggcgc taagccacgg cgttccgctg cgtgacatgg aattcgttca gtatcatcca
    27781 accggtctgc caggttccgg tatcctgatg accgaaggtt gccgcggtga aggcggtatt
    27841 ctggtcaaca aaaatggcta ccgttatctg caagattacg gcatgggccc ggaaactccg
    27901 ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac gcgacaaagt ttctcaggcc
    27961 ttctggcacg aatggcgtaa aggcaacacc atctccacgc cgcgtggtga tgtggtttat
    28021 ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac gtctgccgtt catctgcgaa
    28081 ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac cgattccggt acgtccgacc
    28141 gcacactaca ccatgggcgg tatcgaaacc gatcagaact gtgaaacccg cattaaaggt
    28201 ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg gtgcaaaccg tctgggctcc
    28261 aactccctag cggaactggt ggtcttcggc cgtctggccg gtgaacaagc gacagagcgt
    28321 gcagcaactg ccggtaatgg caacgaagcg gcaattgaag cgcaggcagc tggcgttgaa
    28381 caacgtctga aagatctggt taaccaggat ggcggcgaaa actgggcgaa gatccgcgac
    28441 gaaatgggcc tggctatgga agaaggttgc ggtatctacc gtacgccgga actgatgcag
    28501 aaaaccatcg acaagctggc agagctgcag gaacgcttca agcgcgtgcg catcaccgac
    28561 acttccagcg tgttcaacac cgacctgctc tacaccattg aactgggcca cggtctgaac
    28621 gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag agtcccgcgg cgcacaccag
    28681 cgtctggacg aaggttgcac caagcgtgac gacgtcaact tcctcaaaca caccctcgcc
    28741 ttccgcgatg ctgatggcac gactcgcctg gagtacagcg acgtgaagat tactacgcta
    28801 ccgccagcta aacgcgttta cggtggcgaa gcggatgcag ccgataaggc ggaagcagcc
    28861 aataagaagg agaaggcgaa tggctgagat gaaaaacctg aaaattgagg tggtgcgcta
    28921 taacccggaa gtcgataccg caccgcatag cgcattctat gaagtgcctt atgacgcaac
    28981 tacctcatta ctggatgcgc tgggctacat caaagacaac ctggcaccgg acctgagcta
    29041 ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc atgatggtta acaacgtgcc
    29101 aaaactggca tgtaaaacct tcctgcgtga ttacaccgac ggtatgaagg ttgaagcgtt
    29161 agctaacttc ccgattgaac gcgatctggt ggtcgatatg acccacttca tcgaaagtct
    29221 ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg gtactaacat
    29281 ccagaccccg gcgcagatgg cgaagtatca ccagttctcc ggttgcatca actgtggtct
    29341 gtgctacgcc gcgtgcccgc agtttggcct gaacccagag ttcatcggtc cggctgccat
    29401 tacgctggcg catcgttata acgaagatag ccgcgaccac ggtaagaagg agcgtatggc
    29461 gcagttgaac agccagaacg gcgtatggag ctgtactttc gtgggctact gctccgaagt
    29521 ctgcccgaaa cacgtcgatc cggctgcggc cattcaacag ggcaaagtag aaagttcgaa
    29581 agactttctt atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg actaaacgta
    29641 aaccgtatgt acggccaatg acgtccacct ggtggaaaaa attgccgttt tatcgctttt
    29701 acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg
    29761 ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg attcgtcgac tttttacaaa
    29821 acccggttat tgtgatcatt aacctgatca ctctggcggc agctctgctg cacaccaaaa
    29881 cctggtttga actggcaccg aaagcggcca atatcattgt aaaagacgaa aaaatgggac
    29941 cagagccaat tatcaaaagt ctctgggcgg taactgtggt tgccaccatc gtaatcctgt
    30001 ttgttgccct gtactggtaa ggagcctgag atgattaatc caaatccaaa gcgttctgac
    30061 gaaccggtat tctggggcct cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg
    30121 gtgatgatcc tgctggtggg tattctgctg ccgctggggc tgtttccggg tgatgcactg
    30181 agctacgagc gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg
    30241 atgatcgttc tgccgctgtg gtgtggttta caccgtatgc accacgcgat gcacgatctg
    30301 aaaatccacg tacctgcggg caaatgggtt ttctacggtc tggctgctat cctgacagtt
    30361 gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca atgtaaatcc ggcccgccta
    30421 tggcgggccg ttttgtatgg aaaccagacc ctatgttcaa aacgacgctc tgcgccttat
    30481 taattaccgc ctcttgctcc acatttgctg cccctcaaca aatcaacgat attgtgcatc
    30541 gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg gcggtaattt
    30601 atcagggtaa accttattac tttacctggg ggtatgcgga catcgccaaa aagcagcccg
    30661 tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg
    30721 gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccgcaaca aaatactggc
    30781 ctgaacttac cgctaaacag tggaatggga tcacactatt acatctcgca acctacactg
    30841 ctggcggcct gccattgcag gtgccggatg aagtgaaatc ctcaagcgac ttgctgcgct
    30901 tctatcaaaa ctggcagcct gcatgggctc caggaacaca acgtctgtat gccaactcca
    30961 gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt gagcaggcga
    31021 tgcaaactcg tgtcttccag ccactcaaac tcaaccatac gtggattaat gtaccgcccg
    31081 cagaagaaaa gaattacgcc tggggatatc gcgaaggcaa ggcagttcat gtttcgccag
    31141 gggcgttaga tgctgaaact tatggtgtga agtcgaccat tgaagatatg gcctgctggg
    31201 tacgaagcaa tatgaatccc cgtgatatca acgacaaaac acttcagcaa gggatacaac
    31261 tggcacaatc tcgctactgg caaaccggcg atatgtatca gggtctgggc tgggaaatgc
    31321 tggactggcc ggtaaatcct gacatcatcg ttaacggcag tgacaataaa attgcactgg
    31381 cagcacgccc cgtaaaagcg attacgcctc caactcctgc agtacgagca tcatgggtgc
    31441 ataaaacggg ggcgaccggc ggatttggta gctatgtcgc gtttattcca gaaaaagagc
    31501 tgggtatcgt gatgctggct aacaaaaact atcccaatcc agcgagagtc gccgccgcct
    31561 ggcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg gaccttttct
    31621 gcgcttttcc ttgctgtcat ctacacttag aaaaaaccag taaggaaaca tttatgcgcc
    31681 tgctccctct cgttgccgca gcgacagctg catttctggt cgttgcctgc agttctccta
    31741 cgccgccgcg tggcgtgacc gtagtgaata atttcgacgc taaacgctat ctcggtacct
    31801 ggtatgagat tgcccgtttt gatcaccgct ttgaacgtgg actggaaaaa gtcaccgcaa
    31861 catacagcct gcgtgatgac ggcggcctga atgtcattaa taaaggctat aaccctgaca
    31921 gaggaatgtg gcagcagagt gaagggaaag cgtactttac cggcgcacca actcgcgctg
    31981 cgctgaaagt gtcattcttt ggtcctttct atggcggtta taacgttatt gcactcgatc
    32041 gggaataccg ccatgcgctg gtttgcggac cggaccgcga ctacctgtgg atactctccc
    32101 gcacgccaac catttctgac gaagtgaaac aggagatgct ggcagtcgcg acccgggaag
    32161 ggtttgatgt cagtaagttt atttgggtac agcagcccgg tagttagtga gtgctgagtt
    32221 tcagaccaat aatccccaat acgattaacg ccagactcgc cagacgcatc gggttagcgg
    32281 actcaccgag cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca
    32341 cggcataagc cgtccctact ggtaacgatt tcatcgccca ggcaagtagc gccatactga
    32401 caatcatcgc cgtcacggta ataacactcg gcgtcaaacg actaaagccg tgggtatatt
    32461 tcaggcctac ggcccatacc acttccagca gaccagcaat aactaagata atccaggaca
    32521 tatcaggctc cggagaattg gggccgtccc cggtgaaaag acgcgtttgc aggtcgtcct
    32581 gcaaagctga tggttgaacg gatattttgc caggtggcgg gggaatttca actattacct
    32641 ttgtgactat cgcagaaaaa acaaacggca cgacacagct gtcgtaccgt tattgcttat
    32701 tgctgcgctt tcgttgctgc accagaaatc gcgttaccgc catcagaaat gtcttcacca
    32761 acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc
    32821 gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc
    32881 tatagcaaat taataataga caacatcgcc aaaaatgatg gaaatctgat gatttttcgg
    32941 aagagaagaa aaattagctg gcagcgcggg agatggagtt gccgagatgt ttgatgtctt
    33001 cgccgaaacc gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa
    33061 cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac
    33121 cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga
    33181 cttcgccgat ttgtacgaac agcggaactt taaccacagc gccagtagac agggtagccg
    33241 gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca
    33301 gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac
    33361 actctgcctg atccagcagc catttagcgt tgtcgccaat tgcttttgcg tcagcagaca
    33421 gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag
    33481 tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt
    33541 ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac
    33601 ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa
    33661 gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat
    33721 tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat
    33781 tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat
    33841 atagacgcgg acgaaaaact gttggccgga cgcaacgcca aaaagctgtt tgccctgcgt
    33901 gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc gcttttgcgt
    33961 caggtactta cctcgcaaga tgagtttgtc gtcgcgcccg gattctccac cgacccgctg
    34021 gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg
    34081 ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat
    34141 gccgaaaatc agggcaacaa gcgtaactgg caaactgcac ttgagtatgt tgctgcgcat
    34201 ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag
    34261 ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac
    34321 agtcgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga acgcttttca
    34381 cattctacgc tgcaaatctt gctggtgaat cacatcaacc atgccaatga gatagatgaa
    34441 acattccgtc aggcgatggc taagttgcgc cgggcgggcg ttactttgct gaaccagagc
    34501 gttctgttac gtggtgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg
    34561 ttcgatgcag gcgtgatgcc gtattacctg catgtgctcg ataaagtaca gggcgcggcg
    34621 cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg
    34681 tcgggatatc tggtgccgaa actggcgcga gaaattggcg gcgaacccag caaaacgccg
    34741 ctggatctcc agctacgcca gcagtaactg attaaacagg gaagtttaat ctttgcccgt
    34801 tacataacct ttaattattc atatacattg ctgttgttac tctttatata accaacacca
    34861 ataattaacc tttcttttct gtttatatag agtgagtacg g
//
LOCUS       DAZFTN010000049        34652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFTN010000049 DAZFTN010000000
VERSION     DAZFTN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..633
                     /locus_tag="ACHKV2_002500"
                     /pseudo
     CDS             1..633
                     /locus_tag="ACHKV2_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
     gene            complement(783..2147)
                     /gene="mnmE"
                     /locus_tag="ACHKV2_002501"
     CDS             complement(783..2147)
                     /gene="mnmE"
                     /locus_tag="ACHKV2_002501"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO6940370.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2253..3899)
                     /gene="yidC"
                     /locus_tag="ACHKV2_002502"
     CDS             complement(2253..3899)
                     /gene="yidC"
                     /locus_tag="ACHKV2_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO6940371.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3902..4159)
                     /gene="yidD"
                     /locus_tag="ACHKV2_002503"
     CDS             complement(3902..4159)
                     /gene="yidD"
                     /locus_tag="ACHKV2_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO6940372.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4123..4482)
                     /gene="rnpA"
                     /locus_tag="ACHKV2_002504"
     CDS             complement(4123..4482)
                     /gene="rnpA"
                     /locus_tag="ACHKV2_002504"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO6940373.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4499..4639)
                     /gene="rpmH"
                     /locus_tag="ACHKV2_002505"
     CDS             complement(4499..4639)
                     /gene="rpmH"
                     /locus_tag="ACHKV2_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO6940374.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4869..4949)
                     /locus_tag="ACHKV2_002506"
     CDS             complement(4869..4949)
                     /locus_tag="ACHKV2_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940375.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5246..6649
                     /gene="dnaA"
                     /locus_tag="ACHKV2_002507"
     CDS             5246..6649
                     /gene="dnaA"
                     /locus_tag="ACHKV2_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO6940376.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6654..7754
                     /gene="dnaN"
                     /locus_tag="ACHKV2_002508"
     CDS             6654..7754
                     /gene="dnaN"
                     /locus_tag="ACHKV2_002508"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO6940377.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7754..8827
                     /gene="recF"
                     /locus_tag="ACHKV2_002509"
     CDS             7754..8827
                     /gene="recF"
                     /locus_tag="ACHKV2_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO6940378.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8856..11270
                     /gene="gyrB"
                     /locus_tag="ACHKV2_002510"
     CDS             8856..11270
                     /gene="gyrB"
                     /locus_tag="ACHKV2_002510"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO6940379.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11294..11392)
                     /locus_tag="ACHKV2_002511"
                     /pseudo
     CDS             complement(11294..11392)
                     /locus_tag="ACHKV2_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11510..11908
                     /gene="yidB"
                     /locus_tag="ACHKV2_002512"
     CDS             11510..11908
                     /gene="yidB"
                     /locus_tag="ACHKV2_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO6940380.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12023..12835
                     /gene="yidA"
                     /locus_tag="ACHKV2_002513"
     CDS             12023..12835
                     /gene="yidA"
                     /locus_tag="ACHKV2_002513"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO6940381.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12881..13537)
                     /gene="yidX"
                     /locus_tag="ACHKV2_002514"
     CDS             complement(12881..13537)
                     /gene="yidX"
                     /locus_tag="ACHKV2_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO6940382.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13673..13786
                     /locus_tag="ACHKV2_002515"
                     /pseudo
     CDS             13673..13786
                     /locus_tag="ACHKV2_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13783..14847)
                     /gene="cbrA"
                     /locus_tag="ACHKV2_002516"
     CDS             complement(13783..14847)
                     /gene="cbrA"
                     /locus_tag="ACHKV2_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO6940383.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            14948..16162
                     /gene="yidR"
                     /locus_tag="ACHKV2_002517"
     CDS             14948..16162
                     /gene="yidR"
                     /locus_tag="ACHKV2_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO6940384.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16164..16496)
                     /gene="yidQ"
                     /locus_tag="ACHKV2_002518"
     CDS             complement(16164..16496)
                     /gene="yidQ"
                     /locus_tag="ACHKV2_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6940385.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16736..16808
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16802..17215
                     /gene="ibpA"
                     /locus_tag="ACHKV2_002519"
     CDS             16802..17215
                     /gene="ibpA"
                     /locus_tag="ACHKV2_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO6940386.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17327..17755
                     /gene="ibpB"
                     /locus_tag="ACHKV2_002520"
     CDS             17327..17755
                     /gene="ibpB"
                     /locus_tag="ACHKV2_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO6940387.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17951..19612
                     /gene="yidE"
                     /locus_tag="ACHKV2_002521"
     CDS             17951..19612
                     /gene="yidE"
                     /locus_tag="ACHKV2_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO6940388.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19609..20325)
                     /gene="yidP"
                     /locus_tag="ACHKV2_002522"
     CDS             complement(19609..20325)
                     /gene="yidP"
                     /locus_tag="ACHKV2_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6940389.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20621..22237
                     /locus_tag="ACHKV2_002523"
     CDS             20621..22237
                     /locus_tag="ACHKV2_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6940390.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22237..23559
                     /locus_tag="ACHKV2_002524"
     CDS             22237..23559
                     /locus_tag="ACHKV2_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO6940391.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23556..24449)
                     /gene="yidL"
                     /locus_tag="ACHKV2_002525"
     CDS             complement(23556..24449)
                     /gene="yidL"
                     /locus_tag="ACHKV2_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6940392.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24616..26331
                     /gene="yidK"
                     /locus_tag="ACHKV2_002526"
     CDS             24616..26331
                     /gene="yidK"
                     /locus_tag="ACHKV2_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO6940393.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26328..27821
                     /gene="yidJ"
                     /locus_tag="ACHKV2_002527"
     CDS             26328..27821
                     /gene="yidJ"
                     /locus_tag="ACHKV2_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6940394.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27868..28317)
                     /gene="yidI"
                     /locus_tag="ACHKV2_002528"
     CDS             complement(27868..28317)
                     /gene="yidI"
                     /locus_tag="ACHKV2_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO6940395.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28426..28773
                     /gene="yidH"
                     /locus_tag="ACHKV2_002529"
     CDS             28426..28773
                     /gene="yidH"
                     /locus_tag="ACHKV2_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO6940396.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28763..29125
                     /gene="yidG"
                     /locus_tag="ACHKV2_002530"
     CDS             28763..29125
                     /gene="yidG"
                     /locus_tag="ACHKV2_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO6940397.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29122..29619
                     /gene="yidF"
                     /locus_tag="ACHKV2_002531"
     CDS             29122..29619
                     /gene="yidF"
                     /locus_tag="ACHKV2_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6940398.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29627..30811)
                     /gene="emrD"
                     /locus_tag="ACHKV2_002532"
     CDS             complement(29627..30811)
                     /gene="emrD"
                     /locus_tag="ACHKV2_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO6940399.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30893..30967
                     /gene="ysdE"
                     /locus_tag="ACHKV2_002533"
     CDS             30893..30967
                     /gene="ysdE"
                     /locus_tag="ACHKV2_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO6940400.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31091..31180)
                     /gene="tisB"
                     /locus_tag="ACHKV2_002534"
     CDS             complement(31091..31180)
                     /gene="tisB"
                     /locus_tag="ACHKV2_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO6940401.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31745..31843
                     /gene="ivbL"
                     /locus_tag="ACHKV2_002535"
     CDS             31745..31843
                     /gene="ivbL"
                     /locus_tag="ACHKV2_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO6940402.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31949..33637
                     /gene="ilvB"
                     /locus_tag="ACHKV2_002536"
     CDS             31949..33637
                     /gene="ilvB"
                     /locus_tag="ACHKV2_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO6940403.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33641..33931
                     /gene="ilvN"
                     /locus_tag="ACHKV2_002537"
     CDS             33641..33931
                     /gene="ilvN"
                     /locus_tag="ACHKV2_002537"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6940404.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34194..>34652
                     /locus_tag="ACHKV2_002538"
     CDS             34194..>34652
                     /locus_tag="ACHKV2_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940405.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTV"
BASE COUNT         8382 a         8751 c         9153 g         8366 t
ORIGIN      
        1 aaaatgaatt aaataaagaa gatatattga aactgatggc agcaagagaa aaagtggcgg
       61 gagcatcaga caaatggacg aaggcatcag gcttatattc tgcgatagtg aaagggcata
      121 cgaagattgt tgctgcctgg atggagacag ctgaagtgat agccagccat tatgaaaatg
      181 ataaagatgt agtgagagaa ctcctgtcgc tgagcagaaa taatgcagtt tgctctttgt
      241 atgttgccag ctataagaca atgagtaagc aggtcattga tgtatatctg aatgcggcga
      301 ttcgcctggc gttgcaacac gggttcactt tcgatgagat tttggagcag tttacccgtg
      361 actttgatgg gaagtcattc tctcttgcgg tagagaaagc ggatgatata tatgggtctc
      421 tggctgaaaa tattcaaaat tgtggttggt gaaaatgaaa attatcttaa agatgtgatg
      481 atgcaactgg aagcgaagaa taatgaaggc aaatatgtaa taagcaaggc aaatggcaat
      541 cctgtattta aagaactatt ctggaaagca atagacgaat ttaactttcc gcaagaggag
      601 cttaatcggc taaagcaata tcgttctctg taaagacagg actgcaagat gttgaaagtc
      661 gctccagcca gtagtaaagg cagttttggt gcaggatgct cataaaaaaa caagcgctgt
      721 tccgttctga tagttagttt cccccagaac ggaacagtgc cctttatatc aatttagacg
      781 acttacttac caatacagaa gctggagaaa atccgcccca gcaaatcatc agaggtaaat
      841 tccccggtga tttcgcttaa gttctgctgt gccaggcgca actcttccgc cagcagttcg
      901 cccgcccagg cacccaacag ttgcgcttta ccttgctgca aatgctccgc tgcctgttcc
      961 agcgcctgaa ggtggcgacg acgcgccagg aagccgcctt ccatgttggt gtcaaagccc
     1021 atgctctgtt tgagatggtt acgcagcacg tccacgcctt cgccagtctt tgccgagaga
     1081 cgaatcaacg catgaccgtt cacttcactc attcccagcg tttcgccagt gatatcggct
     1141 ttattgcgta ccacggtgat cggcagtttc gcgggcagac gggcgataaa ttccggccag
     1201 atctccgccg gatcaacggc gtctgtcgtg gtgccatcga ccataaacag cacgcggtcg
     1261 gcttgttcaa tttcttgcca cgcgcgctcg ataccaatac gttccacttc atcgctggct
     1321 tcacgtagcc cggcggtgtc gatgatatgc agcggcattc cgtcaatgtg gatatgctca
     1381 cgcaacacgt cacgcgtggt tccggcgata tcggttacga ttgctgcttc acggccagcc
     1441 agcgcgttta acaggctcga tttaccggca ttaggacgtc cggcaatcac caccttcatc
     1501 ccttcgcgca acaaactacc ctgacgtgct tcagcacgca ctgcatcgag atcggcaatc
     1561 acgtcattaa gctgggcttc aatttttccg tcggagagga aatcgatctc ttcatcgggg
     1621 aaatcaattg ccgcttcgac gtaaatgcgc aagtgggtga gggcttccac cagatggttg
     1681 acccgtgcgg agaatgcgcc ttgcagcgag ttaagtgccg aacgggctgc ctgttccgaa
     1741 ctggcatcga taagatcggc aatcgcctcg gcctgggcta agtcgagttt atcgttaaga
     1801 aacgcgcgtt cggaaaactc accagggcga gcaatccgca ggccgggaat ggtcagaatg
     1861 cgttttaaca gcaggtcgag aatcaccgga ccgccatgac cctgcagttc cagcacatct
     1921 tcgccggtga acgagttcgg gccagggaac catagcgcaa tcccctgatc gagcacgctg
     1981 ccatcggcgt ctttaaacgg aagataatcg gcgtagcgcg gcttaggcag tttacccagc
     2041 acggtttcgg caacttcacg ggctttgaag ccggagatgc gcaggatgcc aacgccgcca
     2101 cgtcccggag gcgtggcctg ggctacgata gtgtcattat cgctcatgat gttcctgttg
     2161 ctttgtgtgg cggatacgcg gtgcttatcc gccctacgaa aagaaaaaag gcggtcaact
     2221 gaccgccctt attttagcga aaactcaccg aatcaggatt ttttcttctc gcggctatgc
     2281 aggccacgtt tttccagacc acggtaaatc agctgctgct gaataatggt taccaggttg
     2341 ctgacgatat agtacagcac cagacctgac gggaaccaca ggaagaacac ggtgaagatg
     2401 accggcataa aggtcatgat cttctgctgc atcgggtcgg tcacagtggt cggcgacatc
     2461 ttctgaatga agaacatcgt tacgcccatc aggatcggca ggatgtagta cgggtcctgt
     2521 gcagacaggt cgtggatcca cagtgcaaac ggtgcctgac gcagttcaac ggaacccatc
     2581 agcatgtagt acaacgccag gaagattggc atctggatca gcagcgggaa gcagccgccc
     2641 agcgggttaa ccttctcagc tttgtacagc gccatcattt cctggctgat acgctgtttg
     2701 tcatcgccca gacgctcacg cattgcctga atcttcggct gcagcatacg catcttcgcc
     2761 atggaggtgt actgcgcttt ggtcagcggg tacatgatgc cacgaacgat aaaggtgatg
     2821 atgataatgg agaagcccca gttacccaca aagctatgga tccatttcag cagtttgaac
     2881 agcggctgag agatgaacca caaccaaccg taatcaacgg tcagatccag gtgcggagca
     2941 acagctgcca ttttgtcctg gatttccggg ccaacccaca gggtgctgtt catcgcgcca
     3001 gtctgaccag gctgaaccag taccggctga gatttatagc cgatagcggc gatgccgtta
     3061 cccagattag cagtatagaa gttgttggta ccgtcgttat gcgggatcca cgccgtcgcg
     3121 aaatactgtt gcagcattgc cacccaacca cctttcgaag agatgttcag gttttcgtta
     3181 tcggcaatgg tatcgaactt gtatttctcg tacttctcgt caggcgtgga gtacgccgcg
     3241 ccacggaagg tgtgcagtgc gaagttgctg cttccggtat cgagatgcgg tggcagagtg
     3301 atggattgct tcaactgacc aaaggtggag atttccagcg gtttctcgcc agcgttctgc
     3361 acgttgtagt tgacgttgac agcgtaatca ccccgtttca ggacaaacgt tttggtaaac
     3421 gtgttgcctg ccgcgtcggt atacgtcatc ggcacctgca gttcgttttg accttcagcc
     3481 agcacataag cgtctttttc aacgttatac agcggacgcg ggccgttagc cgggttatct
     3541 gggccatcac gaccggtcag accgctctgt gcctgataaa taaactgcgg tgaagtttcc
     3601 agcagctgga acggctgggt agagttcagc tctttcgggt aagcaggcag cagagcttgc
     3661 tcaacatcac caccacgggt gttgatggtc agatcaagca cgtcggtctt aaccgagatc
     3721 agtttcccct ggccactggc cggtacgccc tggtcggcgg cgctacccgc tgcggtggtc
     3781 gttgtctgcg tggtctgttg ggcctgaggt tgcgggtttt tatcctgctc ccaggcttgc
     3841 cagatcatga aagacacgaa cagcaaagcg atgactaaaa gattgcgttg cgaatccatc
     3901 gttagtgttc tctggtatta aatggtccgg gcgggacggg atcgtcacca ccagggtgta
     3961 aagggtggca ttttaatacg cgtttcaccg tcaaccaact gccttttatc actccaaacc
     4021 tgcgcaatgc ctcaattccg tagcttgaac aggttggagt gaaacgacaa tgcggcccga
     4081 gtagcggact aatcaggcgt tgatagaccc gaatgagggc tatcaggacc cgcgagccag
     4141 gcgacagtgg cggcgccata atttttccaa cgcttccgag agagcacggt tatcgaggtt
     4201 ggcaacccct tttttcgcca ccaccacgaa atccatagcc gggagttcat gttggcgcag
     4261 acggaagctt tcacgcgtca gacgtttaat ccgattgcgt tcatgggcgc gtcgaacgtt
     4321 tttcttggcg actgtaagac cgatacgggg atgccccagc gaattcaggc ggccgagaat
     4381 ggtaatttgc ggcgtgccag cccgttgtgg ctgctggaag acgaatgtga attgactggg
     4441 agttaacaag cgtaactccc tgggaaatgc gagcttaacc actcaggggt tagctttatt
     4501 acttagaaac ggtcagacga gcgcggcctt tagcacgacg acgtgccaga acctgacgac
     4561 catttttagt agccatacga gcacggaagc cgtgagaacg gttgcgcttc agtacagacg
     4621 gttgaaaagt gcgtttcatg gcgatttcta cctaaacttg aataaattca atggctttat
     4681 tggatatccg ccgaaaaatg aaacgatgga caccgaagcc atgggtgatt aaagaggccg
     4741 gattgtaata attgtacact ccggagtcaa ttctctttcc ttatttaccg cgcttttccg
     4801 caccttttcg cagggaaaac gtacggcctc acaccagtga aaaccagcat ggcgcgccgg
     4861 gtggaggatt atacgggctg atgggtaaag cgcaaggatc gtcctggatc tttattagat
     4921 cgattaagcc aatttttgtc tatggtcatt aaattttcca atatgcggcg taaatcgtgc
     4981 ccgcctcgcg gcaggatcgt ttacacttag cgagttctgg atcatcctgt ggataaatcg
     5041 ggaaaatctg tgagaaacag aagatctctt gcgcagttta ggctatgatc cgcggtcccg
     5101 atcgttttgc aggatcttga tcgggcatat aaccgcagac agcggttcgt gcgtcaccct
     5161 caagcagggt cttttcgacg tacgtcaaca atcatgaatg tttcagcctt agtcattatc
     5221 gacttttgtt cgagtggagt ccgccgtgtc actttcgctt tggcagcagt gtcttgcccg
     5281 attgcaggat gagttaccag ccacagaatt cagtatgtgg atacgcccat tgcaggcgga
     5341 actgagcgat aacacgctgg ccctgtacgc gccaaaccgt tttgtcctcg attgggtacg
     5401 ggacaagtac cttaataata tcaatggact gctaaccagt ttctgcggag cggatgcccc
     5461 acagctgcgt tttgaagtcg gcaccaaatc ggtgacgcaa acgccacaag cggcagtgac
     5521 gagcaacgtc gcggcccctg cacaggtggc gcaaacgcag ccgcaacgtg ctgcgccttc
     5581 tacgcgctca ggttgggata acgtcccggc cccggcagaa ccgacctatc gttctaacgt
     5641 aaacgtcaaa cacacgtttg ataacttcgt tgaaggtaaa tctaaccaac tggcgcgcgc
     5701 ggcggctcgc caggtggcag ataaccctgg cggtgcctat aacccgttgt tcctttatgg
     5761 cggcacgggt ctgggtaaaa ctcacctgct gcatgcggtg ggtaacggca ttatggcgcg
     5821 caagccgaat gccaaagtgg tttatatgca ctccgagcgc tttgttcagg acatggttaa
     5881 agccctgcaa aacaacgcga tcgaagagtt taaacgctac taccgttccg tagatgcact
     5941 gctgatcgac gatattcagt tttttgctaa taaagaacga tctcaggaag agtttttcca
     6001 caccttcaac gccctgctgg aaggtaatca acagatcatt ctcacctcgg atcgctatcc
     6061 gaaagagatc aacggcgttg aggatcgttt gaaatcccgc ttcggctggg ggctgactgt
     6121 ggcgatcgaa ccgccagagt tagaaacccg cgtggcgatc ctgatgaaaa aggccgacga
     6181 aaatgacatt cgtttgccag gcgaagtggc gttctttatc gccaagcgtc tacgatctaa
     6241 cgtacgtgag ctggaaggcg cgctgaaccg cgtcattgcc aacgccaact ttaccggacg
     6301 ggcgatcact atcgacttcg tgcgtgaggc gctgcgcgac ttgctggcat tgcaggaaaa
     6361 actggtcacc atcgacaata ttcagaagac ggtggcggag tactacaaga tcaaagtcgc
     6421 ggatctcctt tccaagcgtc gatcccgctc ggtggctcgt ccgcgtcaga tggcgatggc
     6481 gctggcgaaa gagctgacta accacagtct gccagagatt ggcgatgcgt ttggtggtcg
     6541 tgaccacact acggtgcttc atgcctgccg gaagatcgag cagctgcgtg aagagagcca
     6601 cgatatcaaa gaagattttt caaatttaat cagaacattg tcatcgtaaa cctatgaaat
     6661 ttaccgtaga acgtgagcat ttattaaaac cgctacaaca ggtgagcggt ccgttaggtg
     6721 gtcgtcctac gctaccgatt ctcggtaatc tgctgttaca ggttgctgac ggtacgttgt
     6781 cgctgaccgg tactgatctc gagatggaaa tggtggcacg cgttgcgctg gttcagccac
     6841 acgagccagg agcgacgacc gttccggcgc gcaaattctt tgatatctgc cgtggtctgc
     6901 ctgaaggcgc ggaaattgcc gtgcagctgg aaggtgagcg gatgctggtg cgctccgggc
     6961 gtagccgttt ttcgctgtct accctgccag cggcggattt cccgaacctc gatgactggc
     7021 agagtgaagt cgaatttacc ctgccgcagg cgacgatgaa gcgtctgatt gaagcgaccc
     7081 agttttctat ggcgcatcag gacgttcgct attacttaaa cggtatgctg tttgaaaccg
     7141 aaggtgaaga actgcgcacc gtagcgaccg acggccaccg tctggcggtc tgttcaatgc
     7201 caattggtca atctttgcca agccattcgg tgatcgtgcc gcgtaaaggc gtgattgaac
     7261 tgatgcgtat gctcgacggc ggcgataatc cgctgcgtgt gcagattggc agcaacaata
     7321 ttcgcgccca cgttggcgac tttatcttca cctctaaact ggtggatggt cgcttcccgg
     7381 attaccgccg cgttctgccg aagaatccgg acaaacatct ggaagcaggc tgcgatctcc
     7441 tcaagcaggc gtttgctcgc gcggcaattc tctctaacga gaaattccgc ggtgtgcgcc
     7501 tgtatgtcag cgaaaaccag ctgaaaatca ccgccaacaa cccggaacag gaagaagcgg
     7561 aagagatcct cgacgttacc tatagcggtg cggagatgga aatcggcttc aacgtcagct
     7621 atgtgctgga tgttctgaac gcgctgaaat gcgaaaacgt ccgcatgatg ctgaccgatt
     7681 cggtttccag cgtgcagatt gaagatgcag ccagccagag cgcggcttat gttgtcatgc
     7741 caatgagact gtaatgtccc tcacccgctt gttgatccgc gatttccgca acattgaaac
     7801 cgcggatctc gctttatctc ccggctttaa ctttctggta ggtgccaacg gcagtggcaa
     7861 aaccagcgtg ctggaagcca tctatacgct cggccatggt cgggcgtttc gcagtttgca
     7921 gattggtcgc gtcattcgcc acgagcagga ggcgtttgtt ctccacgggc gattacaggg
     7981 cgaagagcgc gagacggcga ttggcttaac caaagacaaa cagggcgaca gcaaagtccg
     8041 catcgacggt actgacgggc ataaagtcgc ggaactggcg cacctgatgc caatgcagct
     8101 gataacgcca gaagggttta ctttactcaa cggcggcccc aaatacagaa gagcattcct
     8161 cgactgggga tgctttcaca acgaacccgg atttttcacc gcctggagca atctcaagcg
     8221 attgctcaag cagcgcaatg cggcgctgcg ccaggtgaca cgttacgaac agctacgccc
     8281 gtgggataaa gaactgatcc cgctggcgga gcaaatcagt acctggcgcg cggagtatag
     8341 cgccggtatc gcggccgata tggctgatac ctgtaagcaa tttctccctg agttttctct
     8401 gactttctct ttccagcgcg gctgggagaa agagacagaa tatgctgagg tgctggaacg
     8461 taattttgaa cgcgatcgcc agctaaccta caccgcgcat ggcccgcata aagcggactt
     8521 acgcattcgc gccgacggtg cgccggtgga agatacctta tcgcgtgggc agcttaagct
     8581 gttgatgtgc gccttacgtc tggcgcaagg agagttcctc acccgtgaaa gcgggcggcg
     8641 gtgtctctac ctgatagatg attttgcctc tgagcttgat gatgagcgtc gcgggctgct
     8701 tgccagccgc ttaaaagcta cgcaatcgca ggtctttgtc agcgcgatca gtgctgaaca
     8761 cgttatagac atgtcggacg aaaattcgaa gatgtttacc gtggaaaagg gtaaaataac
     8821 ggattaaccc aagtataaat gagcgagaaa cgttgatgtc gaattcttat gactcctcca
     8881 gtatcaaagt cctgaaaggg ctggatgcgg tgcgtaagcg cccgggtatg tatatcggcg
     8941 acacggatga cggcaccggt ctgcaccaca tggtattcga ggtggtagat aacgctatcg
     9001 acgaagcgct cgcgggtcac tgtaaagaaa ttatcgtcac cattcacgcc gataactctg
     9061 tctctgtaca ggatgacggg cgcggcattc cgaccggtat tcacccggaa gagggcgtat
     9121 cggcggcgga agtgatcatg accgttctgc acgcaggcgg taaatttgac gataactcct
     9181 ataaagtgtc cggcggtctg cacggcgttg gtgtttcggt agtaaacgcc ctgtcgcaaa
     9241 aactggagct ggttattcag cgcgagggta aaattcaccg tcagatctac gaacacggtg
     9301 taccgcaggc accgctggcg gttaccggcg agactgaaaa aaccggcacc atggtgcgtt
     9361 tctggcccag cctcgaaacc ttcaccaatg tgaccgagtt cgaatatgaa attctggcga
     9421 aacgtctgcg tgagttgtcg ttcctcaact ccggcgtttc cattcgtctg cgcgacaagc
     9481 gtgacggcaa agaagaccac ttccactatg aaggcggcat caaagcgttc gttgaatatc
     9541 tgaacaagaa caaaacgccg atccacccaa atatcttcta cttctccacc gaaaaagacg
     9601 gtatcggcgt tgaagtggcg ctgcagtgga acgatggctt ccaggaaaac atctactgct
     9661 ttaccaacaa cattccgcag cgtgacggcg gtactcacct ggcaggcttc cgtgcggcga
     9721 tgacccgtac cctgaatgcc tacatggaca aagaaggcta cagcaaaaaa gccaaagtca
     9781 gcgccaccgg tgacgatgcg cgtgaaggcc tgattgcggt cgtttccgta aaagtgccgg
     9841 acccgaaatt ctcctcccag accaaagaca aactggtttc ttctgaggtg aaatcggcgg
     9901 ttgaacagca gatgaacgaa ctgttggcgg aatacctgct ggaaaaccca actgacgcga
     9961 aaatcgtggt tggcaaaatt atcgatgctg cccgtgcccg tgaagcggcg cgtcgcgcgc
    10021 gtgaaatgac ccgccgtaaa ggtgcgctcg acttagcggg cctgccgggc aaactggcag
    10081 actgccagga acgcgatccg gcgctttccg aactgtacct tgtggaaggg gactccgcgg
    10141 gcggctctgc gaagcagggg cgtaaccgca agaaccaggc gattctgccg ctgaagggta
    10201 aaatcctcaa cgtcgagaaa gcgcgcttcg ataagatgct ctcttctcag gaagtggcga
    10261 cgcttatcac cgcgcttggt tgtggtatcg gtcgtgacga gtacaacccg gacaaactgc
    10321 gttatcacag catcatcatc atgaccgatg cggacgtcga cggctcgcac attcgtacgc
    10381 tgctgttgac cttcttctat cgtcagatgc cggaaattgt tgaacgcggt cacgtctaca
    10441 tcgctcagcc gccgctgtac aaagtgaaga aaggcaagca ggaacagtac attaaagacg
    10501 acgaagcgat ggatcagtac cagatctcta tcgcgctgga cggcgcaacg ctgcacacca
    10561 acgccagcgc accggcattg gctggcgaag cgttagagaa actggtgtct gagtacaacg
    10621 cgacgcagaa aatgatcaac cgcatggagc gtcgttatcc gaaagcaatg ctgaaagagc
    10681 ttatctatca gccgacgctg acggaagccg acctctctga tgagcagacc gttacccggt
    10741 gggtgaacgc gctggtcagc gaactgaacg acaaagaaca gcacggcagc cagtggaagt
    10801 ttgatgtcca caccaatgcc gaacaaaacc tgttcgagcc gattgttcgc gtgcgtaccc
    10861 acggtgtgga tactgactat ccgctggatc acgagtttat cactggtggc gaatatcgtc
    10921 gtatctgcac gctgggtgag aaactgcgtg gcttgctgga agaagatgcg tttatcgaac
    10981 gtggcgagcg tcgtcagccg gtagccagct tcgagcaggc gctggactgg ctggtgaaag
    11041 agtcccgtcg cggcctctcc atccagcgtt ataaaggtct gggcgagatg aacccggaac
    11101 agctgtggga aaccaccatg gacccggaaa gccgtcgtat gctgcgcgtt accgttaagg
    11161 atgcgattgc cgctgaccag ttgttcacca cgctgatggg cgacgccgtt gaaccgcgcc
    11221 gtgcgtttat tgaagagaac gccctgaaag cggcgaatat tgatatttaa tggcgttaac
    11281 aatgcgagcg tgcctgatgc gctacgctta tcaggcctac gagactcctg caatatattg
    11341 aatttgcatg tttttgtagg ccggataagg cgttcacgcc gcatccggca agaagctata
    11401 agaaaagggc ggagatcatc tccgcccttt ttatttctgc aatccgcaat tatcatcccg
    11461 cagttctata ctttttgggt caacacgtaa cgctttacag gtgattatca tggggctttt
    11521 tgatgaagtt gtcggcgcct ttctgaaagg cgatgcgggg aaatatcagg cgattttaag
    11581 ttgggttgag gagcagggtg gcattcaggt gctgctggaa aaactgcaaa gtggcggctt
    11641 aggggccatt ctctcaacct ggctgagtaa tcaacagggc aatcaaccag ttagcggcga
    11701 gcaactggaa tcagcactcg gcacaaacgc ggtatccgat cttggacaaa aacttggcgt
    11761 ggataccagt acggcttcct gtttactggc agaacaattg ccgaagatta ttgatgcgct
    11821 ctcaccgcaa ggtgaagtgt caccacaagc caataacgat ctgctttccg caggcatgga
    11881 actgctgaaa gggaaactct tccgctaagc aaatggggag catgcaaccc gcgttgcatc
    11941 ccccttttcc ctcgggtgtt gtactgattt ttgagcggaa tcgcgttagc atgggtcagg
    12001 aaccaatcta cctggggaac tcatggctat caaactcatt gctatcgata tggatggcac
    12061 ccttctgctg cccgatcaca ccatttcacc tgccgttaaa aatgcgattg ccgcagctcg
    12121 cgcccgtggc gtgaatgtcg tgctaacgac gggtcgccct tatgcaggtg ttcacaatta
    12181 cctgaaagag ctgcatatgg aacagccggg cgactactgc attacttata acggcgcgct
    12241 ggtacagaag gccgctgatg gtaacaccgt ggcgcaaact gctctcagct atgacgacta
    12301 tcgtttcctg gaaaaactct ctcgcgaagt cggttctcat ttccacgccc tggaccgcac
    12361 cacgctgtac accgccaacc gtgatatcag ctactacacg gtgcatgaat ccttcgttgc
    12421 caccattccg ctggtgttct gcgaagcgga gaaaatggac cccaataccc agttcctgaa
    12481 agtgatgatg attgatgaac ccgccatcct cgaccaggct atcgcgcgta ttccgcagga
    12541 agtgaaagag aaatataccg tgctgaaaag tgcgccgtac ttcctcgaaa tccttgataa
    12601 acgcgttaac aaaggtacgg gtgtgaaatc actggccgac gtgttaggta ttaaaccgga
    12661 agaaatcatg gcgattggcg atcaggaaaa cgacatcgca atgattgaat atgcaggcgt
    12721 cggtgtggca atggataatg ctattccttc ggtgaaagaa gtggcgaact ttgtcaccaa
    12781 atccaacctt gaagatggcg tggcgtttgc tattgagaag tatgtgctga attaatctgt
    12841 gggcgggcaa acagcataaa cgcgtttgcc cgcttactga ttattggttt actgcatata
    12901 caaccagaaa ttgcagcggc gcagtaatga tgtccagcgt aacagtcact ggcagtaatg
    12961 ctgcggaaac accatccgga atgcctctgg gtgaatagta ttcataaaat cgcactttaa
    13021 atggatggta gaacgccatc acctttgagt aatccatgtt tttatttttc tcatggattg
    13081 tgccttttag gttttccagt gacctggtac agtgttgaac accacaggca aaaccataat
    13141 gcgttgccag tgccttttct tcttcgttat tccagtagta gtaaagcgat attgtgccgg
    13201 taaatttctt tttccccgca tttaaaacaa atcttgcgtc attggcaacc tgaatattgt
    13261 gccggttaag tgctggatca ttgagcatct taacgatgtc atcggcacca tcagtgataa
    13321 ggtaatccag cgattcccct acaaaaacat agccttttgt tccattacta tcttttgcct
    13381 gcgatagccc agtgatggtg tctgactgcg attgatactt tcctgaacta ccctttgcgg
    13441 tatgggaaac cagagcatac gagatacagc ctgaaagcat cagcgccagt gggaataaac
    13501 tggcagcctt aaaaaatccc ttaaaattca acttcatgat taatccttta aacgagtagc
    13561 taagccgttg aaagcatact cttctttccc tcctgtttag cgcctgatct cccttttcag
    13621 atttctgccc gatataagac cttttcttat gaggtgtttt tttgtggact aatatctgtt
    13681 gcaaggctat gggttcgcac ctgcactggt ttatatctcc gccgtcgcgt tgattggcgc
    13741 gctctcttac atcctgctgg tgggcgatgt gacgcgcgtt ggctaatctt tcaactgtgg
    13801 aatatgcgcc ataccactgc gcataatcca ctttcttaaa gccggtgcgg tcaggcatcg
    13861 gctttttact atcttgccga agagttttaa acgcagtttg cgggtggcgt gccagtaggc
    13921 tgcgttgatc ttctctggct gcttcagtaa caccgaacgc agaatctctg cgctatccag
    13981 cgcatagctt atcccctcca gcgagctggc gctgataaat cccgccgctt caccaatcag
    14041 aaaggcgttg tccttaccgc agacaaaatc ctgccagcgt gagggaaata gcaccgtgca
    14101 tttttcgctt tttaccgcct taccaaactg gaattgaaag gcgctcattt tctctttcag
    14161 cgtcgtgaaa cgcgtctgac cgtctttcat tggataggca ccgccaaaga taaaataacc
    14221 gtctttgctg atactccatg agtaacagtc agttatcgca ttatcaaaga tgcatgagta
    14281 gaacggcacc ggatgtttct ctgcgaacca ctgctggata gcgacatatt tacgaatctg
    14341 atggtccggg tagagatatc gccgcaccat cgagttggca ccatctgcac cgaccagata
    14401 gcgggcagta atatgctgct cccaaccgtc tgcacgaaaa atgacatgcc atttatcatc
    14461 ctcacgccag attttccggc acaggctatc gtgataaacc tcaacgctgg cggggatcag
    14521 cgatttcatc cacaagtcga aagcatggcg attaatattg atatagcttc gctggtagtt
    14581 acgcgtgagc gatgcggcga cgtcgacagt tttgacgctg aaaatctgtg gattggcgat
    14641 cacatcgaca ggaagcgtca gtccatcgcg aataaaagat cgctgcgcgt ccggtgccag
    14701 cagaccgcca caaggcttgc tgaaaccttc agtaccatgc tggtgctttt tatccagcgc
    14761 gatcacctgc attttgccgg ctaactttcg tgccaacgcc gaccctgccg ggccgaggcc
    14821 aataatcgcc acgtcgaaat gttccatctt ttcactcaca ttcatcacga tggcaaaaga
    14881 ctattgtctg aaagtgaagt ccttgtgaag tatgatggcg ggaaccgttt tgtatcagga
    14941 ccgcgccatg aaacagataa cctttgctcc ccgtaatcac ctgctcacca ataccaatac
    15001 ctggacgccc gacagccagt ggctggtatt tgacgtgcgt ccttctggcg cgtcgtttac
    15061 cggcgagacc attgagcgtg tgaatatcca taccggcgag gtcgaggtta tctatcgcgt
    15121 gtcacagggc gcgcacgtcg gcgtggtgac cgttcatcca aagtcagaga aatatgtctt
    15181 tatccacgga cctgaaaatc ctcatgaaac atggcattac gatttccatc accgtcgcgg
    15241 cgtgattgtt gaaggcggca agatgaacaa tctcgatgca atggatatta ccgttccgta
    15301 cacatcaggg gcgctgcgtg gcggcagcca tgtgcatgtc tttagcccga acggtgaaat
    15361 ggtgagtttt acctataacg accatgtaat gcatcagttc gattcggcgc tggatttgcg
    15421 aaacgtcggg gttgctgcac cgtttggccc ggtcaacgta caaaagcagc atccgcgtga
    15481 atacagcggt agccactggt gcgtgctggt gagtaaaacc acgcccacgc cgcagcctgg
    15541 cagcgatgaa atcaatcgtg cttatgaaga aggatgggta ggaaatcacg cgctggcgtt
    15601 tattggcgac acactttcgc caaagggcga gaaagtgccg gagctgttta tcgttgagtt
    15661 accgcaagat gaagctggct ggaaagcagc aggtgatgcg ccgttaagtg gaacggaaac
    15721 aaccctgccc gcgccaccgc gtggcatcgt gcagcgacgt ttaaccttta cccaccatcg
    15781 ggcttatccg gggttagtca acgtcccgcg ccactgggtg cgctgtaatc cgcagggtac
    15841 gcaaatcgcg tttttaatgc gagatgataa cggcattgtg caactgtggc ttatctcgcc
    15901 acagggcggc gagccgcgcc agttaaccca taacaaaacg gatattcagt ctgcatttaa
    15961 ctggcatccg tcaggagaat ggttgggctt tgtgctgggt aatcgaattg cttgtgccca
    16021 tgcgcaaagc ggcgaggttg agtatttaac cgaaaaccac gccaatccac cttctgcgga
    16081 cgccgtggtc ttctcgccgg atggtcagtg gctggcgtgg atggaaggcg gccagctgtg
    16141 gatcaccgaa actgatcgct aaatcagtta tcaggcattc tggccggtgg aataaccgca
    16201 ttggtggctt gcgcgttggc ttcgcttttt tcaacgcgtg agcgcaccga gctgtctttg
    16261 cggaacacat cccacggtaa taaaagcgtg tccatcacag cggtaaacgg catatcgaga
    16321 atcgccagcg acttagtgcc ccagttggtt tcatcatcgc caatcatggt ggcgctggcg
    16381 cgggtgccgg gatacgttcc ttctttccct ccggtatggg acatcacgct ggagcatcct
    16441 ccgagtaatg ccattccgct gcatatcatg aacgctaaca acacatttct tatcataata
    16501 aattcatctg ttgatcgtgg gtgttggcct gatgagttat agcgatccct tgctgaaaat
    16561 aacatcatca ttacgtcgct ctgtggcggc tatcgcactt taacgtttcg tgctgccccc
    16621 tcagtctatg caatagacca taaactgcaa aaaaaagtcc gctgataagg cttgaaaagt
    16681 tcatttccag acccattttt acatcgtagc cgatgaggac gcgcctgatg ggtgttctgg
    16741 ctacctgacc tgtccattgt ggaaggtctt acattctcgc tgatttcagg agctattgat
    16801 tatgcgtaac tttgatttat ccccgcttta ccgttctgct attggatttg accgtttgtt
    16861 taaccactta gaaaacaacc agagccagag taatggcggc taccctccgt ataacgttga
    16921 actggtagac gaaaaccatt accgcattgc tatcgctgtg gctggttttg ctgagagcga
    16981 actggaaatt accgcccagg ataatctgct ggtggtgaaa ggtgctcacg ccgacgaaca
    17041 aaaagagcgc acctatctgt accagggcat cgctgaacgc aactttgaac gcaaattcca
    17101 gttagctgag aacattcatg ttcgtggtgc taacctagta aatggtttgc tgtatatcga
    17161 tctcgaacgc gtgattccgg aagcgaaaaa accgcgccgt atcgaaatca actaatttcc
    17221 taaggccgcc ttgcgcggcc tgacatctcc atgctcgccg tcagggagca tatgcgaatc
    17281 ttcggatttg caggtactta ctcgcttctt agaaggagaa atgactatgc gtaacttcga
    17341 tttatcccca ctgatgcgtc aatggatcgg ttttgacaaa ctggccaacg cactgcaaaa
    17401 cgccggtgaa agtcagagct tcccgccgta caacattgag aaaagcgacg ataaccacta
    17461 ccgcattacc cttgcgctgg caggtttccg tcaggaagat ttagagattc aactggaagg
    17521 tacgcgcctg agcgtaaaag gcacgccgga gcagccaaaa gaagagaaaa aatggctgca
    17581 tcaagggctt atgaatcagc catttagcct gagctttacg ctggctgaaa atatggaagt
    17641 ctctggcgca accttcgtaa acggtttact gcatattgat ttaattcgta atgagcctga
    17701 acccatcgca gcgcagcgta tcgctatcag cgaacgtccc gcgttaaata gctaactagc
    17761 tattctcttt gccccgccat tctggtgggg ctttttttgt ctttcttctt caacaatgcg
    17821 ttaaatttcg actgtttaag atatttcggc acgttctttc ccgtgataat gtgcgctata
    17881 aggcaaatct gcttcaccga ttcagccata atccgtagca attaaaatgt tatctcaaga
    17941 gaaatggacg atgagtgata tagcattaac ggtcagtatt ttggctttgg tggcagtcgt
    18001 cggtttgttt atcggcaacg tcaaatttcg cggcatagga ttaggtattg gcggcgtgct
    18061 gtttggtggg atcatcgtcg gccattttgt ttctcaggcg gggatgacat taagtagcga
    18121 tatgctgcat gttattcagg aatttggcct gatcctgttc gtttatacca tcgggattca
    18181 ggtagggccg ggcttctttg cctcattgcg cgtctccgga ttacgcctca acctgtttgc
    18241 tgttctgatc gtcatcatcg gtggtctggt taccgccatc ctgcataaac tgtttgatat
    18301 tccactgccg gtagtgctgg ggattttctc cggtgcggta accaatacgc cagcgctggg
    18361 ggcagggcag cagattttgc gcgacctggg tacaccaatg gaaatggtcg atcagatggg
    18421 gatgagttac gcgatggcgt atccattcgg catttgcggg atattgttca ccatgtggat
    18481 gttgcgggtt attttccgcg tcaatgtcga gacagaagcc cagcagcacg agtcttcacg
    18541 caccaatggc ggcgcgctga tcaagactat caatattcgc gttgagaacc ctaacctgca
    18601 tgatttagcc attaaagatg taccgattct caacggcgac aaaattatct gctcgcgtct
    18661 gaaacgcgaa gaaaccctaa aagttccttc gccagatacc attatccaac tgggcgattt
    18721 gctgcatctg gtgggtcagc cagcggattt acataatgcg caactggtga ttggtcagga
    18781 ggtcgatact tcgctgtcca cgaaaggcac tgatttgcgc gtcgagcgtg tggtggtcac
    18841 caatgaaaac gtgcttggta agcgtattcg cgacctgcat tttaaagaac gctatgacgt
    18901 tgttatctcg cgcctgaacc gtgccggggt cgaactggtc gccagtggcg atatcagcct
    18961 gcagttcggc gatatcctca acctggtggg gcgtccgtcc gcaattgatg ccgttgccaa
    19021 tgtgctgggg aatgcgcagc aaaaactgca acaggttcag atgctgccag tgtttattgg
    19081 catcgggctt ggcgtattgt taggttctat tcccgtcttt gtgccaggat tcccggccgc
    19141 gttgaaactg gggctggcgg gcggtccgct gattatggcg ttgatcctcg ggcgtatcgg
    19201 cagtatcggc aagctgtact ggtttatgcc gccaagtgcc aacctcgcgc tgcgggaact
    19261 ggggatcgtg ctgttcctct cggtcgttgg tctgaaatct ggtggggatt ttgtgaatac
    19321 cctggtcaat ggcgaagggc taagctggat tggttatggt gccctgatca ccgccgttcc
    19381 gctgattact gttggcattc tggcgcggat gttagccaaa atgaattacc tgaccatgtg
    19441 cgggatgctg gcaggttcca tgaccgatcc tccggcgctg gcgtttgcta ataatcttca
    19501 tccaaccagc ggtgcggcgg cgctctctta cgccactgtc tatccgttag tgatgttcct
    19561 gcgcattatc accccccaat tactggcggt gctcttctgg agtatcggtt aactcttgtc
    19621 cgggtggagt cgccgtaaat ggtactccac ctggtattcg ctggcatttc tgaacatcac
    19681 tgaataattc aaaaactccc cgctctcgct atatgacaac gaggtgatcc gcagcagtgg
    19741 cgtgtgttcc gctaccttca tttgccgcgc cagcagtcta tcggcgagca ccggcgtcag
    19801 gctttcataa ttaccgccaa tcaaaatccc gcactcttgt tcaatatatt caaacttcga
    19861 cccttccaga tgttgcagcg aaaggttacg gaacagtttt accggcatat agctgtcttc
    19921 cagcatcagc ggtttccctt ccacaaaacg aacgcgacgg gagaagtaga tctgctcgtt
    19981 gatttgaatc cgcaactggc tggcaattgc cggaggcgca ggcatgattt caaaaatcag
    20041 cacctggctg gtgactgttt ttccctgccg ttttaacacc tccaccagcc cggtcaggct
    20101 ggcggtttga tgcagcacat ctttgcgcac caggtaagtg ccgctgccgt ggcggcggac
    20161 caccagcccc cacgctacca gcaggtcaat cgctttacgg atggtcatcc gcgataccgc
    20221 aaactcttcc gccagctttt tttcaccggg aagaaggctg tttagcgtga aatccgcgga
    20281 attaagtcga attcttaacc gctccgcaat gcttttgtag atcatctcta gatctctttt
    20341 ttgcctgcaa gcccgggtaa tgcgcccaca agttctgctt aatcaattga aaaaacatct
    20401 tttttttaaa gatgtgttcg atgctgtgta ccagtgctca cagagatcta ctttttcgcg
    20461 aaaaagtaga tctctaccgc ccaacgaaaa gcatgaaagc gatcacgaat cccattgggt
    20521 cgtcatgttc tgccgatcgc atcttcctat cctcgctcca ggcctgccgc ataaccaatc
    20581 aggcttccta cttacagaat tgagaaaaga ggatgtggaa atgctcagtc aaattcaacg
    20641 ctttggcggc gcgatgttca cgccagtgct gctgtttccc ttcgccggga ttgtggtggg
    20701 tcttgccatc ttgctgcaaa acccgatgtt tgtcggggaa tcactgaccg atccgaacag
    20761 tttattcgcg caaatcgtac acattattga agagggcggt tggacggtat tccgtaatat
    20821 gccgctgatt tttgctgtcg gtttacccat tggccttgct aagcaagcgc aggggcgtgc
    20881 ttgtctggcg gtgatggtga gtttcctgac ctggaactat ttcatcaatg cgatgggaat
    20941 gacctgggga agctacttcg gcgtcgattt cactcaggac gcggtggcag gtagcggtct
    21001 gacaatgatg gccgggatta aaaccctcga taccagcatt atcggcgcaa ttatcatttc
    21061 cggcattgtg acggcgctgc ataaccgtct gttcgataaa aaactgccgg tttttctcgg
    21121 cattttccag gggacgtctt atgtggtgat tatcgccttc ctggtgatga tcccctgtgc
    21181 ctggctgaca ttgctcggct ggccaaaagt acaaatgggg attgaatctc tgcaagcgtt
    21241 cctgcgttcg gcgggtgcgc ttggggtgtg ggtttacacc ttcctcgaac gtattctgat
    21301 cccaaccggt ttacaccact tcatctacgg accgtttatc tttggtccgg cagctgttga
    21361 aggcggaatt cagatgtact gggcgcagca tctgcaagag ttcagtttga gcgccgagcc
    21421 gctgaaatcg ttgttcccgg aaggaggttt tgccctgcac ggtaactcaa aaatctttgg
    21481 tgccgtgggc atttctttag cgatgtactt cactgccgca ccggaaaatc gggtaaaagt
    21541 ggcgggtttg ctgattcccg caaccttaac cgccatgctg gtgggaatta ccgaaccgct
    21601 ggaatttacc ttcctgttca tttcaccgtt gctgtttgcg gtacacgctg tgcttgcggc
    21661 ctcaatgtcg accgtgatgt atctctttgg tgtggtgggc aacatgggcg gaggtctgat
    21721 tgaccaggtt ttaccgcaaa actggatccc gatgttcagc aaccacgcgg atatgatgct
    21781 gacccaaatc gccattgggt tgtgctttac cctgctgtac ttcgtggttt tccgcaccct
    21841 gattctgcaa ttcaacatgt gcacgccggg acgtgaagat gcggaagtga aactctactc
    21901 aaaagccgaa tacaaagcct cgcgaggcca aaccaccgcg gcagagccaa aaaaagagct
    21961 ggatcaggct gccggtatcc tgcaagccct gggcggggtc ggcaatatct ccagcattaa
    22021 caattgtgcg acgcgtctac gcattgcact gcatgacatg tcacaaacgc tggatgacga
    22081 agtctttaaa aagctgggag cgcacggcgt cttccgtagt ggcgatgcca ttcaggtgat
    22141 cattggtctg catgtatccc agctgcgtga acagctcgat agcttaatta attctcatca
    22201 atcagcagaa aatgttgcca ttacggaggc agtataatga ccaaattctc agtggttgtc
    22261 gcaggcggtg gaagcacctt tacgccaggc atcgtgttga tgctcctggc gaatcaggac
    22321 cgtttcccgc ttcgtgcact gaaattttat gataacgatg gttcgcggca ggaagtgatt
    22381 gccgaagcct gtaaagtcat ccttaaagaa aaagcgccgg atattgcgtt tagttacacc
    22441 accgatcctg aagtggcgtt cagcgacgtt gattttgtca tggcgcacat ccgcgtcggc
    22501 aaatacccga tgcgcgaact ggatgaaaaa atcccgctgc gccacggcgt tgttggtcag
    22561 gaaacttgcg gacccggcgg aatagcgtac ggcatgcgtt ccattggcgg cgtcctggaa
    22621 ctggtggatt atatggaaaa atattcacca aacgcctgga tgctcaacta ctccaacccg
    22681 gcagcaattg ttgcagaagc cacgcgtcgt ctgcgcccga atgcgaaaat cctcaacatc
    22741 tgtgacatgc caatcggtat tgaaagccgg atggcgcaaa ttgttgggct gcaagatcgc
    22801 aaacagatgc gcgtgcgcta ctacggcctg aaccactttg gctggtggac atcaattgaa
    22861 gatttgcagg gcaacgacct gatgcaccag ctgcggcaat atgtctctaa gcacggttat
    22921 gttccaccgc agcaagatac acatactgaa gcgagctgga acgacaccta tgcaaaagcg
    22981 cgggatgtcc aggcactgga cccggataca ttgccgaaca cctatctgaa atattatctc
    23041 ttcccggatt atgtcgttca gcattccaac cctgaacata ctcgcgcgaa tgaggtaatg
    23101 gaacatcgcg agaaacaggt tttcgatgct tgccgcgcca ttacggcggc aggaaattca
    23161 gcggcgggca agctggaaat tgacgaacat gcgtcataca tcgtcgatct ggcggcggca
    23221 attgccttca acactcagga gcggatgttg ctgattgtgc ctaacaacgg ggcaatccat
    23281 aactttgatg atgaagctat ggtcgagatc ccgtgtctgg tcgggcacaa cggaccagaa
    23341 ccactggtgg tcggcgatat cccgcagttt cagaaagggt taatgagtca gcaagtggcg
    23401 gtggaaaaac tggtcgtgga cgcctgggaa cagcgttcat atcagcacct gtggcaggcg
    23461 attacgttgt cgaaaacggt accgagcgcc tcggtcgcca aagctattct ggatgagttg
    23521 ctggaggcca acaaagcgta ctggccagag ttacgttaat tgtgtggact ggcatccgtt
    23581 gggtgctggt ccgtattctt tagcacggct gaaggcgcat aaccaaattt atgtttaaag
    23641 gctttgctga aatgaaagga gtcgaagaag ttgagcgtat cagcgacttc gccaaccgag
    23701 tttccttgtt gctttaatag cgatagcgcc agatccagac gcgcatcgag gtaatattct
    23761 ttcggcgtct ttccggtata gcgtaaaaac agacgacgca accaggcttc gctacagggg
    23821 atcgtggcag ccatatcggc tacgctccag cgttgttgca gactggcatg taaagtggca
    23881 attaattttt caatttgccg ccgttgtgga tcttttttac cgtctgcgta cataagacaa
    23941 atccactgat aaataatttt cgtcagaaaa gcgactgcca gattattttt tattgcttct
    24001 ggtgaagtta ttaactcagc aacttcggtg agttcctgat tataaatttc gccattgtaa
    24061 ataacgcttt gctgaccgat gggaatatcc atcatactgg tgggggtaaa ttccatccag
    24121 tattgttccc agactaaacc ttcacagtga taagagtgaa tatccattgg ctttaaaaat
    24181 ataatacagt tgccatgcag tgttatttgt tcgccattct ttaaaagaat cttcccacat
    24241 ccttgtacgg tatacaccgc aacccatgag ttggcgataa gcggtttctt cttgtcccgt
    24301 ggccagatca cccggtaatg ctcgtcggcg taggtatgcc agagcgagat cagagaaatt
    24361 ccgctggaga tcacattttc gttaatcaat aaaggaatat tgtatggagt ttcgtttttt
    24421 acatctgaag tttgcaattt tccattcatt ttaatatccc tccggcgaat tatgctcaca
    24481 acctgaactt aacagaaaac aatccgttca gggatacgac gtttgtattt taagaatctg
    24541 actgcctgac ccgacgcatt ttacctctcc ctatattcat gcgtccggga cataaacata
    24601 aataagggct atgagatgaa ttcgttacaa atcttgagtt ttgtcggttt tacgctgctg
    24661 gtggcgatca tcacctggtg gaaggttcgc aaaacagata ccggatcgca acaaggctat
    24721 tttcttgccg gacgttcact aaaagcgccg gttattgccg cttcgttaat gttaaccaac
    24781 ctttccacgg aacaactggt tggtctttcc gggcaggcct acaaaagcgg catgtcggtg
    24841 atgggctggg aagtgacttc agcggtgacg ctgatcttcc tcgcgctaat ctttttaccg
    24901 cgctatctga agcgcggcat tgccaccatc cccgattttc tggaggaacg ttatgataaa
    24961 acgacgcgta tgatcatcga cttctgcttc ctcattgcca ccggcgtctg ctttctgccg
    25021 attgttctct actccggcgc gttggcgctc aacagcctgt ttcacgtcgg ggaatcgcta
    25081 cagatttctc acggtgcggc tatctggcta ctggtaattt tgcttggtct ggcgggaatt
    25141 ttgtatgcgg tgatcggcgg actgcgcgca atggcagtgg cggactccat caacggtatt
    25201 gggctggtta tcggcgggtt gatggtgccg gtatttggct tgatcgcgat gggcaagggc
    25261 agctttatgc agggcattga gcaaattacc accgttcacg ccgagaaatt aaactcagtc
    25321 ggtggcccga ccgatccctt gccgattggc gcggcattta ccggtttaat tctggtgaac
    25381 accttttact ggtgtacaaa tcagggcatc gtgcaacgca cgctggcgtc aaaaagcctg
    25441 gcggaagggc aaaagggggc gctgttaacg gcggtgctga aaatgctcga cccgctggta
    25501 ctggtgctgc cagggttgat tgcgtttcat ctgtatcagg atctaccgaa agccgacatg
    25561 gcctacccga cgctggtcaa taacgttctg ccagtgccac tggtgggttt cttcggcgcg
    25621 gtgttatttg gtgcagtgat cagtaccttc aacggctttc tgaatagcgc cagtacgtta
    25681 ttcagtatgg gtatttaccg tcgcatcatt aaccagaatg ccgagccgca gcagctggtc
    25741 accgtcgggc gcaaatttgg tttctttatc gctatcgttt cggtgctggt agcgccgtgg
    25801 atcgccaacg cgccgcaggg gctgtatagc tggatgaaac agctcaacgg catttacaac
    25861 gtgccgctgg ttaccatcat cattatgggc tttttcttcc tgcgcatccc ggcgctggcg
    25921 gcaaaagtgg cgatggggat tggcataatc agctacatca ccatcaacta tctggtgaag
    25981 ttcgacttcc atttcctcta tgtgctggcc tgtacgttct gcatcaacgt ggtcgtgatg
    26041 ctggtgatcg gttttatcaa accgcgcgcc acgccgttca ccttcaaaga tgcgtttgcg
    26101 gtggacatga aaccgtggaa aaacgtcaag atcgcgtcaa ttggcatcct gttcgcgatg
    26161 attggcgtct atgccgggct ggctgaattc ggcggctacg gtacgcgctg gttagcgatg
    26221 atcagttatt tcattgccgc cgtagtgatt gtctacctga tttttgacag ctggcggcat
    26281 cgtcacgacc cagccgtaac ctttactccc gacgcgaagg atagcctatg aaacgcccca
    26341 attttctgtt catcatgacc gatacccagg cccccaatat ggtcggttgc tatagcggta
    26401 aaccgctgaa tacgcaaaat attgatagtc tggcggcgga aggtattcgc tttaattccg
    26461 cctacacctg ttcaccggtt tgtacaccgg cgcgcgccgg gctgtttacc ggtatctacg
    26521 ctaaccagtc cgggccgtgg accaacaacg tcgcgccggg taaaaacatc tccactatgg
    26581 ggcgctactt taaggatgcc ggctatcaca cctgttacat cggcaaatgg catctcgacg
    26641 gtcatgacta tttcggcact ggcgagtgtc cgccggagtg ggacgctgat tactggttcg
    26701 atggggcgaa ctatcttagc gaactgacgg aaaaagagat tagcctgtgg cgcaatggcc
    26761 taaacagcgt cgaagattta caggcgaacc atattgacga aaccttcacc tgggcgcacc
    26821 gcatcagcaa tcgagcggtg gattttctgc aacagcccgc gcgcgccgag gaacccttcc
    26881 tgatggtggt ttcgtatgat gagccgcatc acccgttcac ctgtccggtg gagtatttag
    26941 agaaatacgc tgatttttac tacgagctgg gcgagaaagc acaggatgac ctggcgaaca
    27001 aaccagaaca tcaccgctta tgggcgcagg cgatgccatc gccagtcggt gatgacgggc
    27061 tttatcacca tccgctctat tttgcctgta atgactttgt tgatgaccaa atcggacggg
    27121 tcatcaatgc cttaaagcca gagcaacgtg aaaatacgtg ggttatttat acctccgatc
    27181 acggcgaaat gatgggcgca cataagctga tcagtaaagg ggcggcgatg tatgacgaca
    27241 tcacccgcat tccgctgatc atccgttcgc cgcaagggga gcggcgacag gtcgatacgc
    27301 cagtcagtca tatcgattta ctgccgacaa tgatggcgct ggcagatatt gaaaaaccag
    27361 agattctgcc gggggaaaat atccttgcag tgaaagagcc acgcggcgtg atggtggaat
    27421 ttaaccgcta cgagattgag catgacagct ttggcggttt tattccggtg cgttgctggg
    27481 tgacggatga ctttaaactg gtgctcaacc tcttcaccag tgatgaactt tacgatcgcc
    27541 gtaatgatcc aaatgaaatg cataacctga tcgatgatgc ccattttgcc gacgttcgca
    27601 gcaaaatgca tgatgcctta ttggattata tggacaaaat tcgcgatccg ttccgcagtt
    27661 accaatggag cctgcgtccg tggcgtaaag atgcacagcc gcgctggatg ggggcatttc
    27721 gtccacgccc acaagatggt tattcgccag tggtacgcga ctatgacacc ggcctgccga
    27781 cacaaggggt gaaggtggaa gagaaaaaac agaagttctg acgataatgc ccgctgatga
    27841 acacccggcg ggcattattc aggctgctta aaccagtgaa ccgccagtca ggaaggaaaa
    27901 gataaggaat atcagcaaaa tgctgcacac gatgccgata ccaaaaagca aggtatggaa
    27961 agccagcgtc ccgccgccaa aaacccgcgc gccgcgaatg ccccagcgat tttccttacg
    28021 cataccgcca ataaatgcgc aaagtagcgc agcaatggcc agtgcgatac cgctttgatc
    28081 gaggatttta tcgacatcca cgtcctcttt tctctccacg gtggtaattt tttcgccttt
    28141 tattccggcg agaagacctc ttttaatttc agccgttttg tccgccacga tgctttcaag
    28201 cgttggcggt ggtgcggaaa aagggccgaa tgaaaagtgg ataatcccca tcattaaagc
    28261 gatggcaccg aatagcatgc ccaatgaact gattttattt tgcgctatga tacccatctg
    28321 acctccttgt agcggatggc aaaagcatat caggaaacgg gcggacgaaa ttcttaaaaa
    28381 aaaccgctaa aggtagagcg ttagtgaaat tgcaaaggag caacaatgaa aatttcccgc
    28441 ctcggagaag cgcctgatta ccgcttctcg ctggctaatg agcgtacttt tctggcgtgg
    28501 atccgtactg cactaggatt tctggcggcg ggggttgggc ttgatcaact tgcgccagat
    28561 ttcgccacgc ccgtcattcg cgaactgctg gcgttgttgt tgtgtctgtt ttccggcggg
    28621 ttagcgatgt atggctactt gcgctggtta cgcaatgaaa aagcgatgcg tctgaaagag
    28681 gacctgccct acaccaacag cttattaatc atcagcttaa tattgatggt cgtagcagtg
    28741 atcgttatgg gactggtgtt gtatgccgga tagccgcaaa gccagacgca ttgccgaccc
    28801 agggctgcag ccggagcgta catcactggc gtggtttcgt accatgctgg gctacggcgc
    28861 gttgatggcg ttggctatca aacacaactg gcaccaggcg ggcatgttat tctggatttc
    28921 gattggcatc ctcgccatcg tggcgctgat cctctggcac tacactcgta atcgcaattt
    28981 aatggatgtc acgaatagcg atttttccca atttcacgta gtccgtgaca aatttttgat
    29041 ctccctcgcg gtgttatctc tcgcaatact gtttgctgta acgcatatac atcaacttat
    29101 cgtatttatt gagagagtcg catgacagga agtcaggtca ttgatgctga agaggacagg
    29161 cataaactgg ttgtagagta caaggatgct ctgcaacctg ccgattttta tcacaatttc
    29221 aaacaacggg gcatccgctc cgtacagctt attccccatc ttgaatttga tgaccggggc
    29281 gatatgacgg ccgcttcggt gacagcagag ctgtggggga aatttttaat tgccctgttt
    29341 gagtgttggg tgcgggcaga tatcagtcgt atctcgattg agctttttga cgccacatta
    29401 caaaaatggt gtggcagcga aaacccgcaa ccgcgccgtg gctgccaggc gtgcgactgg
    29461 catctgctgt gtccgcacgc tcgggaggag acgccagaca gcgtgctttg tgcgggctat
    29521 caggcgtttt attcttactc tgcgccgcat atgcgggtga tgcgtgatct gataaagcag
    29581 catcgttctc ccatggagct gatgacgatg ctgcggtgac gtgagcttaa acgggctgcc
    29641 cctgatgcga catccgcgtc gccagcggta gccagcacag cacgatcaac aatcccatta
    29701 aggtcatcaa caaccccagg ctgccctgac cggtttgcgg caacatcgca gagagcgacg
    29761 ccagcacgcc ggaaccaatg ttttgtagac cgccgaccag cgcgccagcc gtgcccgcca
    29821 ggaaagggaa cggctccatc gcgccgctgg tcgccagcgg aaacagcatc ccggcaccga
    29881 aaaagaacag cgcggcggga acgagcagcg tccagacatt catcacgcca aaccagtcgg
    29941 ggatccacat cagtaagcca gccagcaggc agcagataac cgactgccac atcaacgttg
    30001 agaagcgttt attaggacgt ccggcaaacc atgccccaaa aaatgccgcc ggaatcggca
    30061 gaataaacaa aatactgacc gtcatactgc tcagccctaa caccgcgccc atcagcacgc
    30121 cggagcaggc ttcaaaggcg gcaatcccgg ccagaccgcc aatcagcatc agcaaataac
    30181 agttaaaacc gctgttaccg aaaagcgttt tataactggt aagcaggcgc gtgcgcggtg
    30241 cgtcgaccgg acgcgtttcc ggcatccagc gggccatact gaaggtcaca ccggcacaaa
    30301 gtaccaacaa aaagagataa caggcgcgcc agttccacat cgtatccagc agaccgccga
    30361 ttagcggtgc gagcaacgga ctgacaagaa ttcccatgtt taacaggctg ttagcgtggc
    30421 gcaactgtgt ccgttcatat aaatcacgcg gcaaagtacg cgccattacg ccgccaacgc
    30481 cggtgcccat cccctgcatc gcgctggcgg caatcaatac tgtcaaactg gaggtcgtga
    30541 ccgcgaccag cgttgccagc ataaaaatgg acattccgac gaggatcacc ggtcggcgac
    30601 ccacacggtc ggaaatcggg ccataaaaca gctgtgagac accgtaagtc agcagataag
    30661 cgcccattac gctctgcacc gccccttcac gaacgttgag atcgcgcgcc atatcggcaa
    30721 tagctggaat ataaatggtt tgcgccatct gaccgacggc cacgagtaat accaacatca
    30781 ataacaaatt gatgtttcta tgccttttca ttatcacgga tgcttttata aaaataatta
    30841 gaataagtca ctacgcattc ccataaatgc gggagaggaa tctatcatat tgataaatgt
    30901 tagccagata tacgccagga atggggaatt gtttagcggc agaatatgta aacaaaagcg
    30961 gcaataaatg ttgccgggag ggatggtagg tcggataaga tgcgtcagca tcgcatccga
    31021 caccaaccca cacgctaaat ataaaatggg agcggttacc cgctcccctt tagtgcgact
    31081 tgaatctgaa ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa
    31141 tgagtttgag gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag
    31201 gagcactgcc agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat
    31261 ctcaaggaga aacgttgtgc tcgttgacat aacacagtgt gctcacgcgg ggttaaggcg
    31321 ctgacggcac cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc
    31381 ataacacatt gcgtacagtg atattataga agcttgctgt ataaataaac agtaatattt
    31441 ggacaaaacg caaactgtga tcgatactca actatgcact aaatacgtca aaattcgtgc
    31501 cgaaattgcg cgttttgcgc ggaacacgta tactttctgt gttgacataa tacagtgtgc
    31561 tttgcggtta ccagccgcgg gcggctgacg aaacctcgct ccggcggggt tttttgttac
    31621 ctgcagtaca gtacaaaacg tgatcaaccc ctcaattttc ccttgctgaa aaattttcca
    31681 ttgtctcccc tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc
    31741 cccaatggct acttccatgc tcaacgcaaa actactacca actgcgccat ccgccgcagt
    31801 ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac
    31861 gattccaaaa ccccgccggc gcaaaccggg cggggttttt cgtttaagca cctcccggaa
    31921 agtcggccca gaagaaaagg actggagcat ggcaagttcg ggcacatcat cgacgcgtaa
    31981 gcgctttacc ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt
    32041 gacgggcatt ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca
    32101 aatccgccat attctggctc gccatgaaca gggcgcgggg tttatcgctc agggaatggc
    32161 gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc ggaccggggg cgactaacct
    32221 ggtgaccgcc attgctgatg cgcggctgga ctccatcccg ctgatttgca tcactggtca
    32281 ggttcccgcc tcaatgatcg gcactgacgc cttccaggaa gtcgacacct acggcatctc
    32341 tatccccatc accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat
    32401 gagcgatgcc ttccgtattg cgcaatcagg ccgccctggc ccggtgtgga tagacattcc
    32461 taaggatgtg caaacggccg tttttgagat tgaagcacag cccgcggtgg cagaaaaagc
    32521 cgtcgcgccc gcctttagcg aagaaagcat tcgtgacgca gcggcaatga ttaatgctgc
    32581 caaacgcccg gtgctttatc tgggcggtgg tgtgatcaat gcgcctgcga gagtgcgtga
    32641 actggcggag aaagcgcagc tgcctaccac aatgacttta atggcgctgg gcatgttgcc
    32701 aaaagcgcat ccgttgtcgc tgggtatgtt ggggatgcac ggtgtgcgca gcaccaacta
    32761 tattttgcag gaggcggatt tgttgattgt gctcggtgcg cgttttgatg accgggcgat
    32821 tggcaaaacc gagcagttct gcccgaatgc caaaattatt catgtcgata tcgaccgcgc
    32881 agagctgggt aaaatcaagc agccgcacgt agcgattcag gcggatgttg atgacgtgct
    32941 ggcgcagctg atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc
    33001 ggatttgcag cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccgt taagccatta
    33061 cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt
    33121 gggtcagcat cagatgtgga cggcgcaggc ttatccgctc aatcgcccac gccagtggct
    33181 gacctccggc gggctgggca cgatgggttt tggcctgcct gcggcgattg gcgcggcgct
    33241 ggcgaacccg gatcgcaaag tgttgtgttt ctccggcgat ggcagcctga tgatgaatat
    33301 tcaggagatg gcgaccgcca gcgaaaacca gctggatgtt aaaatcattc tgatgaacaa
    33361 cgaagcgctg gggctggtgc atcagcaaca gagtctgttc tacaagcagg gcgtttttgc
    33421 cgctacctat ccgggaaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac
    33481 ctgtgatttg aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc
    33541 tggtccggcg ctgatccatg tgcgcattga tgccgaagaa aaagtgtacc cgatggtgcc
    33601 gccaggtgcg gcgaatacag aaatggtggg ggaataagcc atgcaaaaca caactcatga
    33661 caacgtgatt ctggaactca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg
    33721 cctttttgcc cgccgcgctt ttaacgttga aggcattctt tgtctgccga ttcaggacag
    33781 cgacaaaagc catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag
    33841 ccaaatcgat aagctggaag atgtggtgaa agtgcagcgt aatcagtccg atccgacgat
    33901 gtttaacaag attgcagtgt tttttcagta aggagcgaca ttaaaccgct cgggggagtt
    33961 actgatgagc gatttttgat agatccgtgc agatatatag ggtgaaattg tgccagagac
    34021 catctcaggc acaatttatt agcccaggac gttaatttaa gtcgcagcac cggtaacctg
    34081 ggggggacag ttagggctaa aattttcagc gatggtgtta aattctgatt gctgtctata
    34141 ccagtggtca tgaccagcat tctattaatt aatggttcat cgtgaggtat attatggcaa
    34201 gtcctataaa cagcggaata atgatggcaa atctctgccc ttccaccttt ttggataaaa
    34261 atagaaatgt cgctgctgag cttgatatta aaaataacga gaaaaaatat tcacctggca
    34321 gtaatttcgc aaaatggatg ttgcaggaaa taaagcgctt aatactaaac atcatgtccg
    34381 gttctcggag tattaacacc gatattcttg actatttcca ccctatgcca ggcacggaaa
    34441 ataatggtaa tcgtacctgg gtggcggcaa ctggagagga tgaatatatc gaaatcaaac
    34501 aaacaggtga taagtctttt aatattacac tggttggcag agataaacct tcacgaaaag
    34561 aaattccata ttcaggagtg gctgtagcga ctattattaa atcgttgtct gaaaaaactt
    34621 ctgcgttaga aacacattcc gctgacacgg tt
//
LOCUS       DAZFTN010000050        33446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFTN010000050 DAZFTN010000000
VERSION     DAZFTN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            173..1231
                     /gene="citC"
                     /locus_tag="ACHKV2_002539"
     CDS             173..1231
                     /gene="citC"
                     /locus_tag="ACHKV2_002539"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO6940406.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTESFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1246..1542
                     /gene="citD"
                     /locus_tag="ACHKV2_002540"
     CDS             1246..1542
                     /gene="citD"
                     /locus_tag="ACHKV2_002540"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO6940407.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1539..2447
                     /gene="citE"
                     /locus_tag="ACHKV2_002541"
     CDS             1539..2447
                     /gene="citE"
                     /locus_tag="ACHKV2_002541"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO6940408.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2458..3990
                     /gene="citF"
                     /locus_tag="ACHKV2_002542"
     CDS             2458..3990
                     /gene="citF"
                     /locus_tag="ACHKV2_002542"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO6940409.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3994..4545
                     /gene="citX"
                     /locus_tag="ACHKV2_002543"
     CDS             3994..4545
                     /gene="citX"
                     /locus_tag="ACHKV2_002543"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO6940410.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4520..5398
                     /gene="citG"
                     /locus_tag="ACHKV2_002544"
     CDS             4520..5398
                     /gene="citG"
                     /locus_tag="ACHKV2_002544"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO6940411.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5449..6912
                     /gene="citT"
                     /locus_tag="ACHKV2_002545"
     CDS             5449..6912
                     /gene="citT"
                     /locus_tag="ACHKV2_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO6940412.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7026..7832
                     /gene="rna"
                     /locus_tag="ACHKV2_002546"
     CDS             7026..7832
                     /gene="rna"
                     /locus_tag="ACHKV2_002546"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO6940413.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8062..8472
                     /gene="rnk"
                     /locus_tag="ACHKV2_002547"
     CDS             8062..8472
                     /gene="rnk"
                     /locus_tag="ACHKV2_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO6940414.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8610..8699
                     /gene="yldA"
                     /locus_tag="ACHKV2_002548"
     CDS             8610..8699
                     /gene="yldA"
                     /locus_tag="ACHKV2_002548"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO6940415.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8703..9941)
                     /gene="ybdR"
                     /locus_tag="ACHKV2_002549"
     CDS             complement(8703..9941)
                     /gene="ybdR"
                     /locus_tag="ACHKV2_002549"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO6940416.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10162..10590
                     /gene="uspG"
                     /locus_tag="ACHKV2_002550"
     CDS             10162..10590
                     /gene="uspG"
                     /locus_tag="ACHKV2_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO6940417.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10711..12276)
                     /gene="ahpF"
                     /locus_tag="ACHKV2_002551"
     CDS             complement(10711..12276)
                     /gene="ahpF"
                     /locus_tag="ACHKV2_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO6940418.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12405..12968)
                     /gene="ahpC"
                     /locus_tag="ACHKV2_002552"
     CDS             complement(12405..12968)
                     /gene="ahpC"
                     /locus_tag="ACHKV2_002552"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO6940419.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13340..14086
                     /gene="dsbG"
                     /locus_tag="ACHKV2_002553"
     CDS             13340..14086
                     /gene="dsbG"
                     /locus_tag="ACHKV2_002553"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO6940420.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14296..15198
                     /gene="ybdO"
                     /locus_tag="ACHKV2_002554"
     CDS             14296..15198
                     /gene="ybdO"
                     /locus_tag="ACHKV2_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6940421.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            15345..16565
                     /gene="ybdN"
                     /locus_tag="ACHKV2_002555"
     CDS             15345..16565
                     /gene="ybdN"
                     /locus_tag="ACHKV2_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6940422.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16538..17167
                     /gene="ybdM"
                     /locus_tag="ACHKV2_002556"
     CDS             16538..17167
                     /gene="ybdM"
                     /locus_tag="ACHKV2_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO6940423.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17168..18328)
                     /gene="ybdL"
                     /locus_tag="ACHKV2_002557"
     CDS             complement(17168..18328)
                     /gene="ybdL"
                     /locus_tag="ACHKV2_002557"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO6940424.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18437..19525
                     /gene="hcxA"
                     /locus_tag="ACHKV2_002558"
     CDS             18437..19525
                     /gene="hcxA"
                     /locus_tag="ACHKV2_002558"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO6940425.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19535..19732)
                     /gene="ybdD"
                     /locus_tag="ACHKV2_002559"
     CDS             complement(19535..19732)
                     /gene="ybdD"
                     /locus_tag="ACHKV2_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6940426.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(19914..22019)
                     /gene="cstA"
                     /locus_tag="ACHKV2_002560"
     CDS             complement(19914..22019)
                     /gene="cstA"
                     /locus_tag="ACHKV2_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO6940427.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(22200..22613)
                     /gene="entH"
                     /locus_tag="ACHKV2_002561"
     CDS             complement(22200..22613)
                     /gene="entH"
                     /locus_tag="ACHKV2_002561"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO6940428.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22616..23362)
                     /gene="entA"
                     /locus_tag="ACHKV2_002562"
     CDS             complement(22616..23362)
                     /gene="entA"
                     /locus_tag="ACHKV2_002562"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO6940429.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23362..24219)
                     /gene="entB"
                     /locus_tag="ACHKV2_002563"
     CDS             complement(23362..24219)
                     /gene="entB"
                     /locus_tag="ACHKV2_002563"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO6940430.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24233..25843)
                     /gene="entE"
                     /locus_tag="ACHKV2_002564"
     CDS             complement(24233..25843)
                     /gene="entE"
                     /locus_tag="ACHKV2_002564"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO6940431.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25853..27028)
                     /gene="entC"
                     /locus_tag="ACHKV2_002565"
     CDS             complement(25853..27028)
                     /gene="entC"
                     /locus_tag="ACHKV2_002565"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO6940432.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27217..28173
                     /gene="fepB"
                     /locus_tag="ACHKV2_002566"
     CDS             27217..28173
                     /gene="fepB"
                     /locus_tag="ACHKV2_002566"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6940433.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(28177..29427)
                     /gene="entS"
                     /locus_tag="ACHKV2_002567"
     CDS             complement(28177..29427)
                     /gene="entS"
                     /locus_tag="ACHKV2_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO6940434.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            29538..30542
                     /gene="fepD"
                     /locus_tag="ACHKV2_002568"
     CDS             29538..30542
                     /gene="fepD"
                     /locus_tag="ACHKV2_002568"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO6940435.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30539..31531
                     /gene="fepG"
                     /locus_tag="ACHKV2_002569"
     CDS             30539..31531
                     /gene="fepG"
                     /locus_tag="ACHKV2_002569"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO6940436.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31528..32343
                     /gene="fepC"
                     /locus_tag="ACHKV2_002570"
     CDS             31528..32343
                     /gene="fepC"
                     /locus_tag="ACHKV2_002570"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6940437.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32340..>33446)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKV2_002571"
     CDS             complement(32340..>33446)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKV2_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO6940438.1"
                     /translation="SDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVMAVVFAFACA
                     GLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTEAFNLFIKKF
                     QSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNASKKKDEPSLY
                     TSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFEKEKLAQDRI
                     KMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIE
                     KAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKDGPGKAIIVI
                     LSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         8011 a         9133 c         8423 g         7879 t
ORIGIN      
        1 aatcaaaatt tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata
       61 cctcgatcac ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa
      121 caacattcgt tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg
      181 caatgatatt ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca
      241 attcctgcat gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac
      301 ccgcgatgaa aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc
      361 tatcagtgaa tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa
      421 cctcgcctat gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc
      481 gctgttccgc cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat
      541 ggaaaacagc gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc
      601 agggaacaag attggctgca ttgcgatgaa cgccaatccc tttacgaatg gtcaccgtta
      661 tctgattcaa caagctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga
      721 ttcttcacgc ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat
      781 tccacgcctg actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg
      841 ctacttcatt aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat
      901 tttccgtcag tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaatc
      961 cttttgtcgc gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac
     1021 tatctccgca gcgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc
     1081 gatatccgct tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc
     1141 gctggtccct gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga
     1201 cgcggcagct cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag
     1261 cccgccgttg caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat
     1321 acgcaggata tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt
     1381 cgcaccacca ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc
     1441 gatgataaag gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc
     1501 gccagcggta tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac
     1561 aacgtaaaac tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg
     1621 tcagcaactc cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg
     1681 cattacgtga aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt
     1741 atcgcgatat tgaaaccatt gtgcgtgtaa acgcgctgga ttccgaatgg ggtgttaacg
     1801 acctggaagc cgtcgttcgc ggtggtgcgg acgttgtacg tctgccgaaa accgataccg
     1861 ctcaggatgt tctggatatc gaaaaagaga tcctgcgtat agaaaaagcc tgtggtcgtg
     1921 aacccggcag caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag
     1981 tggaaatcgc tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg
     2041 tgcgcaacct gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt
     2101 ccattttgca ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg
     2161 ctaacaacga agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg
     2221 gcaaatcgct gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc
     2281 agaaagaagt ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag
     2341 gcctcggcgt ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgtgccc
     2401 gtctggtgct ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg
     2461 acgcagaaaa ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct
     2521 gaatgcgatc ttgccgcttt ccagaactca ccaaagcaaa cctaccaggc tgaaaaagcg
     2581 cgcgatcgca aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac
     2641 ggcatgacgg tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg
     2701 atggacgtca tcgcgaagat gggctttaaa aacctgatcc tggcgtccag ctccctgagt
     2761 gattgccatg cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc
     2821 tccggcctgc gtggtccact ggcggaagag atctcccgtg gtctgctggc ggaaccggtg
     2881 caaatccact ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgac
     2941 gtggctttcc tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt
     3001 aaagcctgct gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtt
     3061 gtgatgctta ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat
     3121 caggttgatt taatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt
     3181 gctacccgta tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg
     3241 attgtcaact caggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgca
     3301 tcgctggcgg taaccctttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac
     3361 ttcgccctcg gtggtattac cgcgacgatg gttgatctgc acgaaaaagg tctgatccgc
     3421 aaactgctgg atgtacagag ctttgacagc cacgctgcgc aatcgctggc ccgtaacccc
     3481 aatcacatcg aaatcagcgc caaccagtac gctaactggg gttcgaaagg tgcatcggtt
     3541 gatcgtctcg acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac
     3601 gtgctgaccg gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg
     3661 attgcctctg cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg
     3721 gtggataacg tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac
     3781 cacggtatcg cagttaaccc ggcacgtccg gaactggcag aacgtctgca ggaagcgggc
     3841 attaaagtgg tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca
     3901 cagccgattg aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg
     3961 atcgatgttg tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc
     4021 caccatgcgg tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa
     4081 cacgtctggc tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg
     4141 ccgattaaag acagtgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt
     4201 gccttagccg caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc
     4261 gggccggagg gtatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt
     4321 gagcttgaac atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa
     4381 ggcgaaattc tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag
     4441 caaagcgcag ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc
     4501 atggaggcac tgctgaacga tgtcgatgcc tgcaacgtta actaaaacca caaagcttgc
     4561 gacgtcatta atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa
     4621 tctgtcaccg aaaccaggtc tcgtggatcg cattaactgc ggtgcgcata aagatatggc
     4681 gctggaagat ttccaccgca gcgcgctggc gattcagggc tggctacccc gtttcattga
     4741 atttggtgcc tgtagtgcgg aaatggcacc agaagcggta ctccacggat tacgcccaat
     4801 tggtatggct tgcgaaggtg atatgttccg cgccaccgcg ggcgtaaaca cgcataaggg
     4861 cagcattttt tctttagggc tactgtgtgc ggcaattggc cgtttgcttc agctcaacca
     4921 gttgctaacg ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga
     4981 tcgcgaactg cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct
     5041 tggccttacc ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc
     5101 cttgccgcat tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga
     5161 taccttgctc ctactgatgg cgatcaacgg cgataccaac gttgcttcgc gcggtggcga
     5221 ggggggcctg cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg
     5281 aacccccgca gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct
     5341 cagtccaggc ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt
     5401 atttaagaac ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca
     5461 aaagataata tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc
     5521 cctgtccccg acggtatgcc gccgcaggca tggcattact tcgctgtgtt tgtggcaatg
     5581 attgtcggca tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact
     5641 atttgcgtta ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg
     5701 tttaatgcgc aaaaacaggc gctgaaatgg ggcctggctg gtttttccag cactacggta
     5761 tggctggtat ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt
     5821 cgcattgccc ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg
     5881 attgtcatta tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg
     5941 ggtacggttt ttccggtcat taaaaacctg ccgccgttgt ttaaatcatt cccgaacgat
     6001 ccgtccgcgc gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg
     6061 agttcgtcca tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc
     6121 aaaattgccg gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg
     6181 gttatcttgc ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca
     6241 cacagtgaag aagtggcaac ctgggcgggt gatgaactaa aaaccatggg tgcgctgaca
     6301 cgcagagagt ggacgctgat tggccttgta ttgctcagct taggtttgtg ggtatttggc
     6361 agtgaagtca ttaatgctac tgcggttggt ctgctggcag tttcgctaat gctggctctg
     6421 cacgttgtgc cgtggaaaga cattacccgc tataacagcg catggaacac gctggtcaac
     6481 ctggcaactc tggttgtgat ggctaacggc ctgactcgtt ctggttttat tgactggttc
     6541 gccggtacca tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg
     6601 gttctggtgt tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc
     6661 atgctgccgg ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg
     6721 tgtatcctgc tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt
     6781 cctggggtga ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc
     6841 gcaatcttcg gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc
     6901 atgtggaact aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggttagccc
     6961 acctggcggg cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac
     7021 ccattatgaa agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct
     7081 cttctgccaa cgccgtagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc
     7141 tggccctctc ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac
     7201 gagatgaatg tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg
     7261 gtctgtggcc aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga
     7321 tgcgcttcgg ttgcgctact cgcccaatcc cgaatctacc agaagcgcgc gccagccgga
     7381 tgtgttcatc gccggaaacc ggattatcac tggaaacggc cgctaaacta agtgaagtca
     7441 tgccaggagc tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct
     7501 gctttggttt tgatccggac gcatacttcg gtacgatggt acgcctgaat caagagataa
     7561 aagagagcga agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg
     7621 actttgacgc cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa
     7681 cgtgccaggg taaccctgcg tatttgactg aaattcaggt ctcgatcaaa gctgacgcca
     7741 tcaacgctcc gctttctgca aactcattct tgccacaacc acacccaggt aactgtggca
     7801 aaacctttgt gattgataaa gcgggttatt aatcctctga tttacaaaac acgctaattc
     7861 gtctttcata aaccatgacg attaagaatt atacataatg cgctgcattg aggaattccc
     7921 tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa
     7981 accctcgccg cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag
     8041 taccgacgac atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc
     8101 gaacgcatcg atattctgct ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg
     8161 ttaaacgcag agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg
     8221 gtgacaatga acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc
     8281 acgctggtgt atccggcaaa aatgaccgat agcaatactc agctttccgt tatggctccg
     8341 gtaggtgccg cactgctggg actgcgcgtt ggcgattcca ttcactggga acttccgggc
     8401 ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgac
     8461 tacctgcttt aatcccatct gtacaagcga taactcgctt tgcccagagg atgcactcgc
     8521 atcctcttta tgcaaaatca gcaatttttc ctggttctca ctgttcactt ttgctgcaaa
     8581 attattccgt ttcgtttgtg ggaataacca tggcagaagc attttacatc ctgataggat
     8641 ttctgattat ggcggcgatt atcgtcatgg ctgtccttta cctcgaaaac cactcctgac
     8701 gctcatattg ttccccccgg catcgcattc accagacctg aaaccgcctt ctgcgccgcc
     8761 tctgcgcttt gtgcaccggg taccagaatc accttacggc actcctcttc acgcttttca
     8821 aagatctcat atccccgggc ggcctcttca aacggcatat agtgggtaac aatttcttct
     8881 ggtttcagta atcctttctc aattaacggt aataattctc ccagccatgc gtgaacgtgg
     8941 gtctgtccca ttttaaacgt caacccttta tcaaaggcgt cgccaaacag gaaaccgtga
     9001 ataaatccag cgtagacgcc cggtacgcta acaatgccgc cacgcctgac cgccgcaata
     9061 cactgacgca acgctttacc gctgctgccc tccagtttca ggttagtcag caccgtttcc
     9121 gtggtgctgc ctttcgcttc aaaaccgacg gcgtctatta ctgcatccac gccccggtga
     9181 cctgccgttt gttcaataat tgactgtgcc ggatcgctat cttcatcgaa attaatcggg
     9241 atcgcgccgt agcggtcagc ggcgaaacgc aagcggtagg gatgatgatc aacaacaaaa
     9301 atctgttccg caccgagcaa ccgtgcacag gcgattgtca acaatcccac aggaccggca
     9361 ccatagacag caacgcttga accttgttgg atctgcgcat tttttgctgc ctgccatgcc
     9421 gttggcagaa tatcagaaag gaaaagggct ttatcatctg aaagcaaagg cggtacttta
     9481 aacggcccca cattcccttt agggacacgg acatactccg cctgtccacc aggaacgcca
     9541 ccatacaggt gactataacc aaacaatgcc gcgggagctg gtatctgttt tttattgagc
     9601 gcagcgcctt tacccgcatt ggtattttcg caggcggcat attgctgcag tcgacagaaa
     9661 aaacagtcgc cacaagcaat gacgaacgga attaccaccc ggtcgccttt ttgcaaattt
     9721 tttacatcct ttccggtttc aactacttcc cccataaatt catgaccaaa aatatcgcca
     9781 tgtttaacct ggggtatttt gcctcgataa agatggaggt cagagccaca gatcgccgtt
     9841 gccgttatac gcagaataat atcatctgcc tgttcaatgc ccggatcggg aacattttct
     9901 acctgaacgt gatgtgggcc gtgataagtc aatgctttca tgcaacctcc agagacagtt
     9961 caaaaaagag tcagtcttta agggtagcta tcacagcggg aaaacgggcg gtaccgcagg
    10021 ttttgggaga attcctggtg ttcagtgaga atatattcat cacagaaaca gttcgtaagg
    10081 aaaaagaacc agacaaatat cagggataaa tattataatc atggcgaatg atttgttcat
    10141 gattaacagg gagaaagggt tatgtataag acaatcatta tgccagttga tgtatttgaa
    10201 atggaattga gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt
    10261 attcatctac ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct
    10321 gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag aagaacgttt gcaaacgatg
    10381 gtcagccact tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg
    10441 cgggatgaag tcaatgagtt agcagaagaa ctgggggctg atgttgtagt tattggttct
    10501 cgcaacccat cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccac
    10561 gccaatctgc ctgtactggt cgttcgctaa cagataaaag tatttatgcc gccctgcctg
    10621 gaacatcccg gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc
    10681 taagcaattg caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc
    10741 aaaagcactc agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg
    10801 aaccgtcgta cagtcacccg ctgcgaacac gcctttcacg ttggtttcgc atttcgcatc
    10861 aatgataatc tcgcccatgc ggttacgttc gactgcgcct tcgagccagt tggtgttcgg
    10921 cagcagacca atctgcacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac
    10981 acgatctcga tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg
    11041 cgcattcaga ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc
    11101 ggctttcatt tctggcgcaa attccagcag cgttacgtgc tcaacgatac ccgccaggtc
    11161 aattgccgct tccacgccgg agttaccgcc gccgataacc gctacgcgtt tacctttaaa
    11221 cagcgggccg tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc
    11281 cggaacgttc atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag
    11341 taccgcgcca gaagctgttt caatctgatg cagaccacct tcaactgctg ctgggatcag
    11401 tttgctggcg ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag
    11461 tgcgcctgcc agcttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac
    11521 ggtatcgagg atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg
    11581 tgcggagtaa attgctgccg ctgcacccgc cgggccggaa ccgacgatta atacgtcata
    11641 agcatcacgc ttgttcagct cttctgccgc acgtttttcc gcgcccgtat caattttggc
    11701 aacgatttca gtcaaagtca tgcggccctg accaaactct ttcccgttca cgaacactgc
    11761 cggaacgccc atcacgttac gatcagtgat ctcgttctgg aaggtgccgc cgtcaattgc
    11821 agtgtgcttg atgcgcgggt tcaatacgct catcaggttc agcgcctgta ccacgtccgg
    11881 gcagttatgg caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg
    11941 aatctgctcc agcagagact gcgcttcttt cgacggatga ccaccggtcc acagtaacgc
    12001 cagtaccagc gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt
    12061 agaacctggg ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt
    12121 aaaggtgact ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga
    12181 tttagcgctg tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta
    12241 agccttgagt tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat
    12301 catcatgcaa gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga
    12361 aaatcgggtg ccgcagcacc cgattaatta cgcgattgcg gaacttagat tttaccaacc
    12421 aggtccagag acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca
    12481 cctgggtgag aagctacgta ctgtgctgct ttgattttac gcagcaggtc agacgcgtca
    12541 cggccaatgc cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg
    12601 aaggtcgcac ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg
    12661 gcgccagtcg ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg
    12721 tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt
    12781 ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta
    12841 aagtcagccg ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt
    12901 tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc
    12961 aaggacatct atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttaccga
    13021 ttcggctaat gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac
    13081 aactttgtga actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt
    13141 gaccgtgtaa tctgaactac cctttggtat cttcagcgcc agtctgcagg ctgcgttgtt
    13201 gctcttacaa atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac
    13261 ctgatctaag ctcttaccta tgacaatgat aggttatgcc ttttactcga cttttgcact
    13321 gactgaaaag gacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat
    13381 cgccttcgca gaggaacttc ctgctccagt aaaagcgatt gaaaaacagg gcattacaat
    13441 catcaaaacg ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat
    13501 gggcgtcacc atctacctga ctccagatgg taagcacgct atctctggtt acatgtacaa
    13561 cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg
    13621 acgcgaaatg tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc
    13681 gccggtcatt gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca
    13741 gcaggcgcgc ccgtgggtag attctggcaa agtacagttg agaacattgt tggttggggt
    13801 tatcaaacca gaaagtccgg cgacagccgc ggcaattctt gcctccaaag atcccgcaaa
    13861 aacctggcaa gaatatgaag cctctggtgg caagcttaag ctaaacgtgc ctgcaaacgt
    13921 aagtacagag caaatgaaag tgttaagcga caacgagaaa ctcatggacg atctgggggc
    13981 aaatgtcact ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg
    14041 gttgcccgat cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg
    14101 atggttgaac tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctctg
    14161 ccctaagtgg ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaaacataa
    14221 tcaataaaaa tatatacgca aaaaacattg attaagtgaa tatatcatgg aagaaaaata
    14281 taaccggagt agtgtatgac caatctctac gacttgaaaa agttcgactt aaatcttctt
    14341 gtcatatttg agtgtattta ccagcattta agcattagca aagctgctga atcactatat
    14401 atcaccccat cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct
    14461 ttattcattc gctcaggaaa aggtatcgcg ccgacgacaa cagggttaaa tctgcatcac
    14521 catcttgaga aaaatctcag aggccttgaa caaacaatta atatagtcaa taagtccgaa
    14581 ctaaagaaaa actttattat tcacggccct cagctcattt cttgctctaa taatagtatg
    14641 ctgatccgct gcctacggca agattcctct gtcgagattg agtgccatga tatcctcatg
    14701 tcagcagaga atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa
    14761 atgccagtga tcagccgttc cgtcatttgc atgccattgc atacgattcg caatactcta
    14821 atttgtagta ataggcatcc acgaattact gacaatagca cttatgaaca aattatggca
    14881 gaagagttca cccaattaat atctaaatca gcaggggttg atgatattca aatggaaata
    14941 gacgagagat ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaatcatt
    15001 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taactcccac
    15061 cgtgactttc ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt
    15121 tacatttcct ataataaatc ttctttaaac aatctggtgt tctcacgatt tattgaccgt
    15181 ctaaatgagt gtttttgata tataacctca gacagctact ataactttca cctatttatt
    15241 cacagcgcga atgaaaaata aaacactctg cttttcagtg tttactttat aattaacagt
    15301 cttatctcac tgatcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa
    15361 ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg acactcaaca
    15421 ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc
    15481 tgacagccga actcgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg
    15541 aggcgcaatt ctcttgcacc attaactatg ttcagtccct gcgcgagttg tacaccgatg
    15601 tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat
    15661 accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtacgtcag cccccgcaag
    15721 atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc ttcgaacaat
    15781 ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca
    15841 tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt
    15901 ttgccgacga taaaccctgg actacggccg caccaggcgg tcatagctgg tacatttacc
    15961 ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat caaagtctct
    16021 gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat atgcgaattt
    16081 gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt atcgaaccgg
    16141 atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg
    16201 gacatgataa tcatttctat gggcatcgga aaatcctcaa gccagaacat ttagactggc
    16261 aagaatatgc attattgctg ctcaatagca tgccggaaaa aacagctgag cattaccgca
    16321 ataaaatcgc catttatttg cactggtatc agaaaaaagg catcgaggtt ccacaaaccc
    16381 agcaagggga cattggcgcg aaagatatcc cctcctggcg gcggatatgc aaagttttac
    16441 tcaataacga ttactggtgt cgggcattat catttagtcc tacgaaagcg aagaactatc
    16501 agcgttataa cgaacggata aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg
    16561 attaacgcag gatttaacgc aatttctcgc cagcttgccg gaagatgatc gcatcaacgc
    16621 gattaatgaa atccgcatgg cgatccatca ggttagcccc tttcgtgaag agccagtgga
    16681 ttgcgttctg tgggttaaaa acagccaact tatgcctaat gattacaacc caaacaacgt
    16741 ggcaccaccc gaaaaaaaac tgctgaaaaa atccattgaa atagatggtt ttacccaacc
    16801 cattgtggtc acgcatacgg ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca
    16861 tgaaattggt aaaggctcat catcattaaa attacgctta aaaggttatt tgccggtgac
    16921 ctgtctggaa ggtacgcgta atcaacgcat cgccgcgact attcgtcata atcgcgctcg
    16981 tggacgccat caaatcaccg ccatgtcgga aatcgtccgt gaactcagtc agttgggatg
    17041 ggacgataat aaaattggca aagagctggg aatggacagc gacgaagtgc tgcgcctgaa
    17101 gcaaattaac ggtctgcaag agctgtttgc cgaccgtcaa tattcccgcg cctggactgt
    17161 caaataacta cagctggcgc agtcgttcag ctgctgccag caacgtcgat tccttcttgg
    17221 caaaacagag acgaatcagt ttatgtggga agggatcggc gcaaaacacc gatagcggaa
    17281 tcgccgccac gccgtgctcc cgcgtcagcc actggcaaaa ctcaacatca tccagggtag
    17341 aaaccgcgct gtaatccacc agcaaaaagt atgtgccttc gcacggtaaa atctccagcc
    17401 gactttcatt taaggcattt accagaatat cgcgcttctg gcgataaaag tccggtaacg
    17461 caagataatg ctcaggttct gcacgtagca tatccgcaat tgccagctgt gccggggtat
    17521 tcaccgaaaa ggtcagatac tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg
    17581 caacacaata acccactttc cagccggtca tatgataggt cttgccaaac gatgacaccg
    17641 ccaccgcccg ctcacgcagc tgcggatgcg ccagcacact ggcatggccc tgttgtgaaa
    17701 agttgatgtg ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct
    17761 gccacaaagc ggcgaaatca gcctgctgcc agacagttgc actgggatta tgcggagtgt
    17821 tgaggatcac cagtctggtg cgctcgctta acaatgcggc aaattcctgc cagtcaacgc
    17881 gaaaatgcgg tggttgcagt gccatacgct tcactattcc cccagaaagc gcgatggcgg
    17941 gggcgtaact gtcatagctg ggatcaaaac aaatcacttc atcgccattg cgcaccagtg
    18001 cggtaatcgc cgcgtataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg
    18061 catctggttg atagccatac aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct
    18121 gcacgccggt catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct
    18181 cctgtaaata gcgcggacca tcaaaatcag gaaagccttg cgacaggtta atcgcctggt
    18241 gttgctgcgc cagcgcgctc atctgggtga aaatagtggt gccaagttgt ggaagtttgc
    18301 tttgtggaat cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa
    18361 ggcactataa cacgatgcta gtatttggca atcaagacgt ttagatgtct aaataaaaca
    18421 ataaggacaa cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta
    18481 cttttcacat ccaggaagtt tcaatcacct gcacgatttt ttcactgatg aacaactttc
    18541 tcgcgcggtg tggatctacg gcgtacgcgc cattgctgcg gcgcaaacca aacttccgcc
    18601 agcgtttgga cttccagggg caaagcatat tttgtttcgt ggtcattgca gcgaaagcga
    18661 tgtacagcaa ctggcggctg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg
    18721 cggcgcacta ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc catttgttgc
    18781 cgctccaacg atcgccgcca cctgcgccgc ctggacaccg ctctccgtct ggtataacga
    18841 tgccggacag gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga
    18901 accggagatt atcctcaatg cgccgcaaca atatctgctg gcggggatcg gtgatacgct
    18961 ggcgaaatgg tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt
    19021 gcgactgggg atcaataatg ctcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca
    19081 ggcgcttgcc gatcagcaaa atcagcagtt aacgcaatca ttttgcgatg tggtggatgc
    19141 cattattgct ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtagcggc
    19201 ggcccatgcc gtacataacg gtctgactgt cctgccacaa accgagaagt ttctccacgg
    19261 caccaaagtc gcctacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct
    19321 ggcgcaatta accggggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga
    19381 agtggatatc aataatcagg cggagatcga taaagtgatt gcccacacct tgcgcccggt
    19441 ggagtccatt cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga
    19501 aaaagtggaa tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc
    19561 caccgtagcg cgcgtcctgc cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat
    19621 cgggatggtt aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca
    19681 gcttcgccgc ctgacctaaa tattttccgg cttttgccag tgaatcaaac atcttggcct
    19741 ccgctaacca tgttgctgaa agagtgccgg atgcggcgtg aacgccttat ccggcctaca
    19801 aatgctatca acgcgctcgg tcagccccct cgccccatcg gggagagggt tagggtgagg
    19861 gggaacccac ggcgccatgc caacattcgc caacatcccc cctcactctg actttagtgc
    19921 gcgccttttg cctgcaccac gatctcctcg acattttccg gcattggctc atacggcgtt
    19981 tctttcgccg ttggcttcgg ctctttcaat gccgcaagtg ccgtcttaat cgagaacagt
    20041 gccagaacca ccacgaccac cataaagaag atggttaacc cggcatccag acggttgttg
    20101 aacaccagtt gcgccagctg tgactcagta tactgcgatg gaatattacc gctgtcgatc
    20161 attgcctgga acttattagc aatggccagg aagccgactt tcgcatccgg gctaaacgct
    20221 ttctgccagc ctgcggtcag ggtacaaatc agcagccagg ccgttggtac cagcgccacc
    20281 caggcgtaac gttgacgttt catcttgaac aacaccacgg cacagagcat cagcgccatc
    20341 cctgccagca tctggttggc aataccaaac agcggccaca gagtgttaat accgcccagc
    20401 ggatcgacca caccctgatg caggaagtag ccccacgcca gcacgcacag cgccgttgcc
    20461 agcaggttag caggcagtga atcggtccgt ttcaggcccg gagaaaccac gcccagcaga
    20521 tcctgcaaca taaagcgcgc agcacgcgta cctgcatcca ccgccgtcag aataaacaga
    20581 gcttcaaaca gaatggcgaa gtgataccag aacgccacat ccatcatgcc gcccagcgcg
    20641 ccatgcagaa tgtaggccat ccccaccgcc aacgtcggcg caccgcccgc acgggaaatg
    20701 atcgactgtt ctcccacttc gctggcaatc tggttgagcg tatccggagt aatcgcaaag
    20761 ccccagctac tcaccacttg cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc
    20821 agcaccgcca tcgggctgtt catggcgaag tacacgcccg gatcgatgat acaggcggaa
    20881 accagtgcca taatcgccac gaaggattcc attaacattc caccgtagcc gataaaacaa
    20941 gcctgccctt cgttcgccag catcttcggc gtggtgccgg aagagatcag cgcatggaag
    21001 ccagacaccg cgccacaggc gatggtgata aacaggaacg ggaacaggtt accggtccat
    21061 accgggccag tgccgtcaac aaatttggtc aacgcaggca tggtcagcgt cgggcgcata
    21121 atcaaaatgc ctaccgccag accaacgatc gtcccgattt tcaggaaggt agagaggtag
    21181 tcacgcggag ccagcagcaa ccataccggc agcaccgccg ccacaaaacc gtaacccacc
    21241 agcatccagg tcagctgcac gccggtaaag tcaaagtacg gtgcccaggt cggactttct
    21301 gccacccagc cgccagaaat aatggcgaaa atcaggaata ccagaccgat gaccgacact
    21361 tcaccaatac gccccggacg cagatagcgc aggtagatcc ccatgaacag cgccagcgga
    21421 atggtgaacg caacggtata cgttccccac gggctatgag tcagggcttt caccacgatc
    21481 atcgccagta ctgcaaggat aatgaccatg atcataaagc aggccaccag cgcgatcacc
    21541 ccggcggttg gccccatctc ttctttgacc agctcaccca gcgaacgacc gtcacggcgc
    21601 gtagaaacaa acagcaccat gaaatcctgc accgcaccgg cgagaaccac cccggcgagt
    21661 agccagatca tccctggcag gtaacccatt tgcgccgcca gtaccggccc aaccaacgga
    21721 cctgctccgg caatggccgc aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca
    21781 tagtccagcc cgtcgttatg gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc
    21841 acatttttgg cgatatacag accataaaaa cggtaagcga tcagatagat acagaccgac
    21901 gccaccacaa tccacagcgc gttgatctgt tccccacgat ttaaagcaat atatcccaga
    21961 gcaaatgctc ccattacaga gagcactgtc cagacgaggt atttccctga tttgttcata
    22021 gttgttatcc gtgtgcgtgt ccatagagat gttacatttt gtttctataa catctttgta
    22081 cgatttaaca acacagcaac catgtaaatc ggagttgaaa ccaatattta acttaacaat
    22141 gttaactcga tcactccgtt aaccgagttt tcctgcaatc tcatataatt ctgtcgggtt
    22201 catcccaaaa ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa
    22261 acgacgattt cccagctctg attttgccgc ccaagatgca gcggctggca gacgccgcgt
    22321 accttgcctt cagacaccgg gcgatgatgt gtggcgttaa gttccgtgcc caccacacac
    22381 tgcccgtcgc gggtcatcat aaatccggcc atcgatccca gcgtttcagc cagcgccgcc
    22441 gacgcgccgc catgcagcag gccaaacggc tgatgagtac ggatatcaac cggcatttcg
    22501 gcttccagca catcatcgcc cagacgggta tacacaattc ccagatgcgc caccattgtg
    22561 ttatcgctgg tggcgttcag ttcgtcgagc gttaaatggc gtttccagat catgcttatg
    22621 cccccagcgt tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt
    22681 cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt ttccccagcg
    22741 gaatgccgag tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc
    22801 tcacccacag cgtgcgttgc atatcggtat cggtggaacc cggcgaaacc acattacagc
    22861 gcacgccgct acccgccagt tccagcccga cgctcaacgc caggcttttc agcgccgctt
    22921 tcgatgcgcc ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag
    22981 tgacaatcgc cccgccccgc tgacggcgaa actggttcat ggtttgctgg aacaggttaa
    23041 acgcaccgcc gacattaacc gcaaaagtct gctgccagtc ctccttactg agctgatcgg
    23101 tcgcgcccat gcgcaaaatt cccgccgcat tgaccagcac gtccagtcgc tccgtttcag
    23161 ctaacagtcg ctgacatacc tgcgccacct gcgcagcatc ggcaacatcc attacttcgg
    23221 tcgcaaaggg atattgctcc tgagcgaacg cttgatcaaa acctgtaact ttcgctcccg
    23281 cttcaacaaa cgccagcgcc gtggcgtagc cgataccttt ccccgcgccg gttacccaga
    23341 catttttacc gctgaaatcc attatttcac ctcgcgggag agtagcttcc accaggcgtc
    23401 gatggtcggg tttttcgcca acatgacaaa gtcgatatcg ccatgcactt tgcgccagcg
    23461 cgccgccagc gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc
    23521 gaacggttca tcggactcgt ccagcaacgg caggatcacc tcacgcagcg ccgctttgct
    23581 ggcggggata ggtgctggca gtaattcttc ggtcatcacc acccggccag aacgtccggc
    23641 cacatatttc agcgacatca aatgctcgtc acggctgaaa tcggccagcg catccgccac
    23701 cataaacggt ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac agccaatgtg
    23761 ggcatatacc ccggtaataa tcagctggtt acgtccactc tctttgagca tttgctccag
    23821 cggagaacga tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg
    23881 cgtcagtcga tccaccacct tttgctgttc cggcgaacgg gtcaggcccg gcccccacat
    23941 atcattcaac agcgcccgat cttcatcgct ctgctctttc ggctgggcgg tgtaataaac
    24001 cgggatattg tgctgtttgc agtagtcgcg cagcgcagca atattcgcga tcacctgctc
    24061 catcatcggg cagttctcgc cccagaagct gacaaaatag tcctgcatat catggattaa
    24121 caacgcggca cgttgcggtt caaaggccca gtcaacttta ttctgcggaa tatcgtgaga
    24181 ctccggcagt gcgtaagcct gtaattttgg aatagccatc gtgttctctc cttcaggctg
    24241 atgcgcgtga cgccagccac tgacgtaatt gttttttatc gactttcccg accgccgtca
    24301 gcggaagtga atccacacac tccacgcgat ccggtaattt aaattcggca ataccctgtt
    24361 cacgcaggaa acgacgcacc tgcaccgcgc gaagcggctc ttttaccacc agataagcac
    24421 agcttttttc gcccatcagc tcatcttcca tactgaccag tgcggcgtag atcaccgccg
    24481 gatggcgcag taacaggttt tcgatctctt cggcagcgat cttctcgccg ccacggttga
    24541 tctgatcttt ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata gagatcagat
    24601 cgccggaaca gtaaaaaccg ttggcatcaa aggcgctggc attgtgctgc gggcttttgt
    24661 aatagccacg gaaggtatac ggcccgcgcg tcatcagtcg tccgacttcc ccttgcggca
    24721 gtggatttcc ttcggcatcg gcaacccata cttcgtcatc cggacacatt gggtaaccct
    24781 gggtatggat aattttctcc gcgctatcat caagacgggt atagttcacc agcccttccg
    24841 ccataccaaa cacctgctgc aactggcaac caatctcagc gggaatacgc gccgcaagcg
    24901 tggcagaaag acgtgcgcca ccgacctgta acagtttcag cgaggcaagc tgcgcccggc
    24961 tttcgccttc ggtcagcgcc tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa
    25021 cgttaatctg atgtttttca atcaatggga agcaaagcgt ggcgctggga tcggccgcca
    25081 gaacaaccgt tcctccggca agaaagacgc ccagcgatcc cggcgaactc atggcgtagt
    25141 tatgagccgc cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga caaatctcga
    25201 cgctacgacg cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac
    25261 cggtggtgcc gccggaaagc tggaaataga ccacttcatc agcaggtgac ggcgtagcag
    25321 taaaaccgtc tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga
    25381 gtagctgcac cacgcgaatg gaagaatgct ctgcaacaaa tgtgttgagg aaatcatccc
    25441 cgctaaacag cgcatgttgg cgatcggcaa tcagcaatgc gggttcaatc tggctggcat
    25501 aggcgttcag ttcactacgc tgatggctga acaacgccag caccggcgca acgcccagtt
    25561 tcagcagcgc gaaaaaggta atgtaaagtt cagcgacgtt acccagttgt accagcgcgg
    25621 tttcaccagg tttaatgccc tgacggcgta aactacacgc gaggttatcc gccgcctgat
    25681 tcagctcccg gtaactcaac tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag
    25741 cgtggcgagt cagaatgtca gtcagtggca aatcctgcca gtagcctttt tcccgatagc
    25801 gacgggcaaa ctcttccggc cagcgggtga atggaatgct catcctcgct ccttaatgca
    25861 atccaaaaac gttcaacatg gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg
    25921 gtgacgacgc aggcacaatc cccgctccgg caaacagacg cacctgattt tcccgcagct
    25981 tcgcgcagcg gatggtcacc acccattcgc cgttaccttc gctgtcacac caacccacaa
    26041 tgccgccaaa cagttcgcgg tcgaacggct ccagttcagc aataacctgg gtcgcggcct
    26101 gatgcgggaa accgctcagc gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt
    26161 tttcttgcga attcgcttta ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg
    26221 taatcaattg tggagaggaa ggaacgtgta actcactact gcgttcgcgc agtacctctt
    26281 tcatcgcctg agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc agcagacgat
    26341 tacccgcttc gcgatcgagc acttcatccg gctgacgacg tgcggaaccg gctaacggaa
    26401 tggagctaaa acgctcaccg tctttacgta gcagcagttc cgggctggcc cccagcagaa
    26461 cgccaccatc tgccagcggg acatggaagt tgtaactaac cgggttttgc gcaatcaacc
    26521 gttccagcaa tacgccacta tcaatggcgg cgtcagtggt gatatcaatc aaccgtgaca
    26581 acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt
    26641 caaacgtggt ttgctccgga attgcctggc gttccaccac gttcagcgac tggctgcggg
    26701 tgaaacggcg tgcggaagct tgtttttcct gacgggagaa cgactgccag gattcaggaa
    26761 tatacagcga cgaaggctga cgtggatcga aaggaatcgc cccgaccatc accggatttt
    26821 tgatgccctg cgctttggca tcggcaaaca gcgcggcgag tttttgctgg aagggactgt
    26881 cgggcgaatc cccgttcaca gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa
    26941 aactgcggta cggcgacata aagaaaaagc gattgggcgc aagtgttgcc atggtctgct
    27001 gtacttcctc agccagtgac gtatccatat catcctccac aaaatgataa aggctttaat
    27061 aatgattatc atttatattt tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact
    27121 cttgaggtaa cgcaatctgc aactcggggt tgcatctggt tgtggttata atgaaaatga
    27181 gaagcattaa cttcattaat aacaggaggc tgatttgtga gactcgcccc gctctaccgc
    27241 aatgcccttc tattaacagg acttttgttt tcaggaatag ccgcagttca ggccgccgac
    27301 tggccgcgtc agattactga cagccgtggc actcataccc tggaaagcca gccgcagcgt
    27361 attgtttcca ccagcgtcac cctgaccggc tcactgctgg cgattgatgc tccggtgatc
    27421 gccagcggcg cgaccacgcc gaataaccgc gtcgcggatg accagggctt tttacgccag
    27481 tggagcaagg tggcgaaaga acgcaaactg caacggctct atatcggcga accgagcacc
    27541 gaagccgttg ccgcgcaaat gccggatctg attttaatta gcgcaaccgg cggggattcg
    27601 gcgctggcgc tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac
    27661 gacaaaagct ggcaggcgct gttaacgcaa cttggcgaaa ttaccgggca tgagaaacaa
    27721 gcggcagagc ggattgcgca gtttgataag caactggcgg cggcgaaaga gcaaatcaaa
    27781 ttaccgccgc agccggtcac tgccattgtc tatactgccg ctgcacacag tgccaatctc
    27841 tggacgccgg aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaag
    27901 ttacccgcag ggttaaatgc cagccaaagc cagggtaaac gccatgacat cattcagctt
    27961 ggtggggaaa atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat
    28021 cagaaagatg ccgatgctat ttatgctaat ccgctgctcg cgcacctgcc tgcagtacaa
    28081 aacaagcagg tttatgcgct gggaaccgag acgttccgtc tggattacta cagcgccatg
    28141 caagtgctgg aacgacttaa ggcgctgttt taagcattaa ccgtcagacg ctgtcacctg
    28201 cggcggcgtc tggcgaaaac gtcgcaactc caccagcacc agcaataaca acacgccgat
    28261 aatcaataaa ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgctcccaa
    28321 accacccagc agcgccgcgc ctatggcatc gcccgtcacg ttttgtgccg tccacaaacc
    28381 gttaatccgc cctaacatcg cttccggggt ttgcgtttgc agcattgtgt attgcagcaa
    28441 cgagctgacc gcactcaacc agccgaacag cgccagacaa accacgccta aaatccacat
    28501 cggcatcagg ccaaacagac caatggcgag gaacgatccc agcgtggaga gcagcatcaa
    28561 taaccctggt cgcgcgctat gcgccagctt cccgctggtt aacgcgccaa tagccgcgcc
    28621 gagcgggatc gccgcataga gaaaaccaat ctgtgccgcc gacatctgcc agttgtcagc
    28681 cagcgccgga tacagtaccc gcaccgcact cgccatcgtc aataaaccac ccagcagcgc
    28741 aatccctccc accagcgggc tggcgagtag aaaacgaaat cctgccagta atgatttcaa
    28801 cggatgctca cgcggctgcg gtggcggtgg cagtgccgga aggcttaaca acggtagcaa
    28861 ggtaataaac gtgcccgccg ccgccagccc gtagttccag gccacaccgc cagtcgccag
    28921 cagtaaaccg ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat
    28981 cgccccggcc tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc
    29041 cgtaacgcca agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa
    29101 tgacggctcc ggcagcagcg cgttcaggca cagtccaatg aagccaatgc cacaggtgcc
    29161 gcgcgccagc aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccgaccat
    29221 caggccaaca aacatcgcgc cgccggtcag cgtcaccgaa agccccacct gccaggtgga
    29281 atgtgtcatc atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat
    29341 tgagatgaaa cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag
    29401 gttaagcagc caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc
    29461 tattcttagg ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca
    29521 ttatcatgga agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg
    29581 gattgctgtt attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac
    29641 tccccgcttc cgtagtgctg gaggccttct ccggcacctg ccagagcgcc gactgcacca
    29701 tcgtgctcga cgcgcggctg ccgcgtaccc ttgccggttt actggcaggc ggcgcgcttg
    29761 gccttgccgg ggcgttaatg caaaccctca cccgaaaccc acttgccgac cccggcttgc
    29821 ttggcgtgaa cgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact
    29881 cttccgcaca ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcgttgattg
    29941 ttgcctttac cggcagccag ggcggtgggc agttaagtcc ggtgcgttta accctggcgg
    30001 gcgtggcgct ggcggcggtg ctggaagggc tgaccagcgg catcgccctg cttaatcctg
    30061 acgtctacga tcaactgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata
    30121 ccttaaaagt ggtgctgatc ccagtgctga tcgccggagc aactgcgcta ttactgagtc
    30181 gcgcgctgaa cagtttaagc cttggcagcg acaccgcgac ggcgctgggc agtcgcgtgg
    30241 cgcgcacaca gttgattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacggcgg
    30301 tagttggccc tattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg
    30361 gtgccgatca tcgctggtcg ctgcccgtca cgctacttgc tacccctgcc ctgctgctgt
    30421 ttgccgatat catcgggcgg gtgattgtgc ccggtgaact gcgcgtttct gtggtcagtg
    30481 cgtttattgg tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat
    30541 gatttacgtc tctcgccgat tactcatcac ctgtttgctg ctgatttccg cctgtgtggt
    30601 tgcaggtatc tggggattac gcagcggtgc cgtcacgctg gaaatctcgc aggtattcgc
    30661 cgcgctgatg ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc
    30721 acgcgtgctg atggcgctgt tgattggcgc ggcactgggc gtcagcggtg cgatttttca
    30781 gtcgctgatg cgtaacccac tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg
    30841 gagcggcgtg ttggtggcga tggtgctgtt tggtcaggat ctgacggcta tcgcgctggc
    30901 agcaatggtg ggcggcatta tcacttcgct gttggtctgg ctgctcgcct ggcgtaacgg
    30961 tatcgacacc tttcggttga ttattatcgg tatcggcgtt cgcgccatgc tggtggcctt
    31021 taatacctgg ctgttgctga aagcgtcttt agaaacggcg ctaacagcag gtttgtggaa
    31081 tgccggatcg ctcaacggcc tgacgtgggc aaaaacctcg ccttccgcac ccatcattat
    31141 attgatgcta attgccgccg ccttactggt acgccggatg cgcttgctgg aaatgggcga
    31201 tgataccgcg tgtgcgctgg gcgtcagcgt cgaacgttcg cgtctgttaa tgatgctggt
    31261 tgcagtggtg cttaccgctg cggcaacagc gcttgccggg ccgatttcct ttattgcttt
    31321 agtcgcgccg cacattgccc gacgcattag cggcaccgct cgctgggggc taacccaggc
    31381 ggcgctatgc ggggcgctgt tactgctggc ggccgatctc tgcgcccaac aactgtttat
    31441 gccgtatcaa cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt
    31501 tttgttaatt caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa
    31561 cagttaaccc tgggatatgg caaatatacc gttgcggaaa atctgactgt agaaatacct
    31621 gatggtcact tcacggcaat tatcgggcca aatggctgcg gtaaatccac gttgctgcgt
    31681 accttaagcc gcctgatgac gcctgctcat gggcatgtct ggctggatgg cgagcacatt
    31741 caacattacg ccagtaaaga agttgcccgg cggattggtc tgttggcgca aaatgccacc
    31801 acgctgggag atatcaccgt gcaggagctg gtggcgcgtg ggcgttatcc acatcaaccg
    31861 ctgtttaccc gttggcgcaa agaagatgaa gaggcggtaa cgaaagcgat gcaggccacg
    31921 ggaataactc atcttgcaga tcaaagcgtg gatacccttt ccggtgggca acgccagcga
    31981 gcgtggatcg cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg
    32041 acctggctgg atatcagtca tcagattgat ttgctggaac tgttaagcga actgaaccgc
    32101 gagaaaggct ataccctggc ggcggtgctg cacgatctta atcaggcctg tcgttacgcc
    32161 agccatttga ttgcattgcg agaagggaaa attgttgctc agggagcgcc gaaggagatt
    32221 gtcactgctg aactgattga gcgcatttat ggtctccgct gcatgatcat tgacgatccg
    32281 gtagccggaa cgccgctcgt ggtgccgctc ggacgaacgg caccttcaac cgcaaatagt
    32341 taaactaagt ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaat
    32401 aacacgctac cacaagccac catcccgccg atcagcgcgg aaaggatcac aataatcgcc
    32461 ttacccggac cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta
    32521 aaattcacat cgttgatatt tgcttttgtt aactgctcaa caagatactg acgattacgt
    32581 aattcaccgt tcagttccgc aacgtcagtg accgcttttt caatttccag tttgcgttca
    32641 ataccgtctg cgccgagaga aatagagaaa tcggggtcat ctttaacggc ctgaccatta
    32701 ctgtaaacgg gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc
    32761 tgaatgtttg catcaagttg atttttcatt ttaatgcggt cctgagccag tttttctttt
    32821 tcaaactggg ttttgatctc cagtttatta cggacgtttt ctatcgactc tttcaccacc
    32881 aacgcagaga tataatcgat atacccgctc aaaacggtct gcgcctcttc actggttggc
    32941 gcggtaaaac ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca
    33001 ttgtcatcaa ccgctttcat tttttcgctc aatgcgacaa ttgcgcgatg caaatccagt
    33061 tcgtcgattt tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct
    33121 tccagcaagc taaccgactg aaacttcttg ataaacaggt taaatgcttc tgtacgatca
    33181 attttgatat ccagatccag cacacgaagc ttagtgaatg ttttctccaa ctcttgccac
    33241 tgaacaggtt ctggaggcgt gacaaccgcc gcgctggtcc atttttgcgg caggatgaaa
    33301 gagatcagca agcctgcaca ggcaaacgca aaaacgaccg ccatgaccgt ttttttggcc
    33361 cgccataaaa ctgagattag attaagtaaa tcaatttcat tattactggg cgacgccaga
    33421 ggataatcgg gaaaatgagc gtcact
//
LOCUS       DAZFTN010000051        32700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFTN010000051 DAZFTN010000000
VERSION     DAZFTN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            142..3894
                     /gene="yfaL"
                     /locus_tag="ACHKV2_002572"
     CDS             142..3894
                     /gene="yfaL"
                     /locus_tag="ACHKV2_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO6940439.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4035..4757)
                     /gene="ubiG"
                     /locus_tag="ACHKV2_002573"
     CDS             complement(4035..4757)
                     /gene="ubiG"
                     /locus_tag="ACHKV2_002573"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO6940440.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4904..7531
                     /gene="gyrA"
                     /locus_tag="ACHKV2_002574"
     CDS             4904..7531
                     /gene="gyrA"
                     /locus_tag="ACHKV2_002574"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO6940441.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7739..9367
                     /gene="yfaA"
                     /locus_tag="ACHKV2_002575"
     CDS             7739..9367
                     /gene="yfaA"
                     /locus_tag="ACHKV2_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO6940442.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9364..9987
                     /gene="yfaT"
                     /locus_tag="ACHKV2_002576"
     CDS             9364..9987
                     /gene="yfaT"
                     /locus_tag="ACHKV2_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO6940443.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10011..14525
                     /locus_tag="ACHKV2_002577"
                     /pseudo
     CDS             10011..14525
                     /locus_tag="ACHKV2_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            14526..16175
                     /gene="yfaQ"
                     /locus_tag="ACHKV2_002578"
                     /pseudo
     CDS             14526..16175
                     /gene="yfaQ"
                     /locus_tag="ACHKV2_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16180..16956
                     /gene="yfaP"
                     /locus_tag="ACHKV2_002579"
     CDS             16180..16956
                     /gene="yfaP"
                     /locus_tag="ACHKV2_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO6940444.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17231..20080
                     /gene="rcsC"
                     /locus_tag="ACHKV2_002580"
     CDS             17231..20080
                     /gene="rcsC"
                     /locus_tag="ACHKV2_002580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO6940445.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20280..20930)
                     /gene="rcsB"
                     /locus_tag="ACHKV2_002581"
     CDS             complement(20280..20930)
                     /gene="rcsB"
                     /locus_tag="ACHKV2_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO6940446.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20947..23619)
                     /gene="rcsD"
                     /locus_tag="ACHKV2_002582"
     CDS             complement(20947..23619)
                     /gene="rcsD"
                     /locus_tag="ACHKV2_002582"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO6940447.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24358..25461
                     /gene="ompC"
                     /locus_tag="ACHKV2_002583"
     CDS             24358..25461
                     /gene="ompC"
                     /locus_tag="ACHKV2_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6940448.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25573..26628
                     /gene="apbE"
                     /locus_tag="ACHKV2_002584"
     CDS             25573..26628
                     /gene="apbE"
                     /locus_tag="ACHKV2_002584"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO6940449.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            26702..27766
                     /gene="ada"
                     /locus_tag="ACHKV2_002585"
     CDS             26702..27766
                     /gene="ada"
                     /locus_tag="ACHKV2_002585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO6940450.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27766..28416
                     /gene="alkB"
                     /locus_tag="ACHKV2_002586"
     CDS             27766..28416
                     /gene="alkB"
                     /locus_tag="ACHKV2_002586"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO6940451.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28492..30135
                     /gene="yojI"
                     /locus_tag="ACHKV2_002587"
     CDS             28492..30135
                     /gene="yojI"
                     /locus_tag="ACHKV2_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO6940452.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLSLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            30353..31999
                     /gene="mqo"
                     /locus_tag="ACHKV2_002588"
     CDS             30353..31999
                     /gene="mqo"
                     /locus_tag="ACHKV2_002588"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO6940453.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRRLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(32149..32637)
                     /gene="eco"
                     /locus_tag="ACHKV2_002589"
     CDS             complement(32149..32637)
                     /gene="eco"
                     /locus_tag="ACHKV2_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO6940454.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         7965 a         8006 c         8871 g         7858 t
ORIGIN      
        1 attatgacgc actgatacta ttcatcaaat aataacaaaa tagctattgc atcgggttgg
       61 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
      121 taaaatgatg gtcagattaa tatgcgggtt atctttctac gcaaggagta tttatctcta
      181 ctcccgtcaa tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta
      241 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
      301 acgcaggtct ggagtattgc cgatgggcaa tggctggttt tttcggatat gaccaataat
      361 gccagcggtg gggccgtatt tttgcaacaa ggagcggaat ttacattatc accagaaaat
      421 gaaactggaa tgactctgtt tgccaataac accgtttcag gagaatataa taacggcggg
      481 gcaatatttg ctaaagaaaa ctcaacgctg aatcttacgg atgttatttt ttctggtaac
      541 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaacgatac cggtgccatc
      601 gatttacgtg tcactaacgc cgtgtttcgc aataacatcg ctaatgacgg caaaggtggt
      661 gcaatttata ccatcaataa tgatatctat ttaagtgatg atgtttttaa caataaccag
      721 gcatatacat caacaagtta cagtgatggc gatggcggcg caatcgatgt cacagataat
      781 aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat
      841 aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt
      901 attgatattt ctgttgatga cagctacagc cagaacggag gcgtgttagt cgatgagaac
      961 aatagcgcag caggctatgg agatggtcct tcctctgcgg cgggtggctt tatgtatctc
     1021 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
     1081 gagaatgacg gagctgttga ctctattgct ggtaccgggt taatcaccaa aacaggttcc
     1141 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
     1201 ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag
     1261 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
     1321 gcagagctaa atgttggctc cacccaacaa acctttgcgc actcattgac gggctttcag
     1381 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct
     1441 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta tgtgctggcg
     1501 ggggcgcagt cgatggcgct aaccggcgat atagtggtgg atgctggtgc ggtgctttcg
     1561 ctggaaggcg acgcggcaga tcttgccgct ctccaggacg atccgcagtc gatcgtgtta
     1621 aacggcggta tgctcgatct ctctgatttc tccacctggc agagcggtac atcatacaaa
     1681 gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
     1741 cttgcaggcg gaaacgatat gcatatcggc ggcgacggga aagatggcgt ctacgtggtg
     1801 atcgatgcgg gtgacgggca ggtcagcctg gcaaatgaca atcaatacct cggcacaacg
     1861 caaatcgctt ccggtacgct gatggtgagc gacaactcgc agcttggata tacccattat
     1921 aaccgccagg ttatctttac cgataagcca caagaaagcg tgatggagat tactgccaat
     1981 gtcgatactc gctctacaac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
     2041 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
     2101 gggcagcatc aggatgaggg tagcacattg actaaaacgg gggcgggtac actggagctg
     2161 accgccagcg gtacaacgca gtcagcggtg agagtagaag agggcacgct gcaaggtgat
     2221 gttgcggata tcttccctta tgcttcgtcg ctatgggtcg gtgacggggc aacgttcgtt
     2281 actggcgcgg atcaggatat tcagtcaatt gatgctactt ccagcggcac tatcgacatc
     2341 agcgatggta cggttttgcg cctgaccggg caggatactt ccgtcgccct taatgcctca
     2401 ctgtttaact gcgatgggac gctggtgaat gccaccgatg gtgtgacgtt gacaggtgag
     2461 cttaatacca accttgaaac tgacagcctg acttatcttt ccaacgtgac ggttaatggc
     2521 aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg
     2581 acggtaaacg gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc
     2641 gatgactcgg taagcgatca attggtgatg aacggtaata ctgctggcaa cacaactgtg
     2701 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
     2761 ttcgcagctg atcccacgca gtttcaaaac aatgcgcagt tcagtctggc aggcagcggc
     2821 tacgtcaata tgggagcgta tgactacacg ctggtggaag ataacaacga ctggtatctg
     2881 cgatcgcaag aagtaacgcc gccatcgcca cctgatccag acccgactcc cgatcctgat
     2941 cccacgcagg atcctgatcc aacacccgac ccggaaccta cgcctgctta ccagccggtg
     3001 ttgaatgcca aagttggcgg ttatctcaat aacctgcggg cggcaaatca ggcgtttatg
     3061 atggagcgac gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg tgttatcggc
     3121 ggagattatc attacacagc agcggggcaa ctggctcaac atgaagacac ttctacggtg
     3181 caacttagcg gcgatctgtt tagcgggcgc tggggcacgg atggcgagtg gatgcttggg
     3241 attgttggtg gctacagcga taaccagggc gacagccgct cgagtatgac cggaactcgc
     3301 gccgataacc agaaccacgg ttatgcggtt gggctgacct caagctggtt tcagcacggt
     3361 aagcagaagc agggggcctg gctggataac tggttgcagt acgcgtggtt tagcaatgat
     3421 gtttctgaac atgaagatgg cgtggatcat taccattcgt cggggattat cgcctcgctg
     3481 gaagcggggt atcagtggtt accggggcgt ggtgtggtga ttgaaccgca ggcgcaggtg
     3541 atttatcagg gcgtgcagca ggatgatttt accgccgcta accgtgcgcg cgtgtcacaa
     3601 tcgcagggtg atgatattca gacgcggctg ggtttacaca gcgaatggcg taccgctgtt
     3661 catgtcatac caacattaga tctgaattat tatcacgatc cccattcgac ggaaattgaa
     3721 gaagatgcca gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat aaaagtggga
     3781 gtcacgggca atatcagtca gcgagtttcg ctgcgtggta gcgtggcgtg gcagaaaggg
     3841 agtgatgatt ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg gtaatcgctg
     3901 tctgaccatc actattgctg gatgcggcgt aaacgcctaa tcaggcctac ggagagcatt
     3961 tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat tggcgcggtt gccggatgaa
     4021 tccaaacaac aacctcactt attctgcgtg tgcagcatat agttcacatc cacgccgggg
     4081 ccgagtttaa aagtattagt gatcgggttg taatgtagcc cggttatatg tcgctcttta
     4141 aggcttgtct gatctaccca gcccagcagt tctgccggtt taataaactt cttcacatca
     4201 tgtgtacctt taggcaccat gcgcaaaata tattccgcgc cgactaccgc catcagccat
     4261 gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt caccagttgc
     4321 gcacaggctc tgaccactga ctgcggatca gggacgtgct ccagcatctc catgcaggtc
     4381 accacatcat actgcccggc atgttttgcc gcgtgctctt ccacggtttc ctgcacgtaa
     4441 tccacctgaa tgccgctttc cagcgcgtgc agctttgcca cctgcaatgg ctcaaagccc
     4501 atatccagac cggtcaccgt cgcgccttcg cgcgccatac tctcggccag aatgccgccg
     4561 ccacaaccga catcgagcac ctttttgcca aataagccgc cagcacgctc ggcaatatag
     4621 ctcagacgca gcgggttaat gcggtgcagc ggtttgaact caccttccag atcccaccag
     4681 cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg gtttaccggc
     4741 gatttttcgg cattcattgg cacttctact ccgtaattgg caagacaaac gagtatatca
     4801 ggcattggat gtgaataaag cgtataggtt tacctcaatc tgcgcggctg tgttataatt
     4861 tgcgaccttt gaatccggga tacagtagag ggatagcggt tagatgagcg accttgcgag
     4921 agaaattaca ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg attatgcgat
     4981 gtcggtcatt gttggccgtg cgctgccaga tgtccgagat ggcctgaagc cggtacaccg
     5041 tcgcgtactt tacgccatga acgtactagg caatgactgg aacaaagcct ataaaaaatc
     5101 tgcccgtgtc gttggtgacg taatcggtaa ataccatccc catggtgact cggcagttta
     5161 tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg tagacggtca
     5221 gggtaacttc ggttccatcg acggcgactc tgcggcggca atgcgttata cggaaatccg
     5281 tctggcgaaa attgcccatg aactgatggc cgatctcgaa aaagagacgg tcgatttcgt
     5341 tgataactat gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa ttcctaacct
     5401 gctggtgaac ggttcttccg gtatcgccgt aggtatggca accaacatcc cgccgcacaa
     5461 cctgacggaa gtcatcaacg gttgtctggc gtatatcgat gatgaagaca tcagcattga
     5521 agggctgatg gaacacatcc cggggccgga cttcccgacg gcggcaatca ttaacggtcg
     5581 tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag gtatatatcc gcgcccgtgc
     5641 tgaagtggaa gttgacgcca aaaccggacg tgaaaccatt atcgtccacg aaattccgta
     5701 tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag aaaaacgcgt
     5761 ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca tcgtgattga
     5821 agtgaaacgc gatgcggtcg gtgaagttgt gctcaacaac ctctactccc agacccagtt
     5881 gcaggtttct ttcggtatca acatggtggc attgcaccat ggtcagccaa agatcatgaa
     5941 cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga cccgtcgtac
     6001 tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat tagccgtggc
     6061 gctggcgaac atcgacccga tcatcgaact gatccgtcat gcgccgacgc ctgcagaagc
     6121 gaaaactgcg ctggtggcta atccgtggca gctgggcaac gttgccgcga tgctcgaacg
     6181 tgctggcgac gatgctgcgc gtccggaatg gctggagcca gagttcggcg tgcgtgatgg
     6241 tctgtactac ctgaccgaac agcaagctca ggcaattctg gatctgcgtt tgcagaaact
     6301 gaccggcctt gagcacgaaa aactgctcga cgaatacaaa gagctgctgg atcagatcgc
     6361 ggaactgctg cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc gtgaagagct
     6421 ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact gaaatcaccg ccaacagcgc
     6481 agacatcaac ctggaagatc tgatcaccca ggaagatgtg gtcgtgacgc tctctcacca
     6541 gggctacgtt aaatatcagc cgctttctga atacgaagcg cagcgtcgtg gcgggaaagg
     6601 taaatctgcc gcacgtatta aagaagaaga ctttatcgac cgactgctgg tggcgaacac
     6661 gcacgaccat attctgtgct tctccagccg tggtcgcgtc tattcgatga aagtttacca
     6721 gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc tgccgctgga
     6781 gcaggacgaa cgtatcaccg cgatcctgcc ggtgaccgag tttgaagaag gcgtgaaagt
     6841 cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc ctcaccgagt tcaaccgtct
     6901 gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc gatgagctga tcggcgttga
     6961 cctgaccagc ggcgaagacg aagtaatgct gttctccgcc gaaggtaaag tggtgcgctt
     7021 taaagagtct tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg gtattcgttt
     7081 aggcgaaggt gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg caatcctcac
     7141 cgcaacgcag aacggttatg gtaaacgtac cgcagtggcg gaatacccga ctaagtcgcg
     7201 tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt aacggtttag ttgttggcgc
     7261 ggtacaggta gatgactgcg accagatcat gatgatcacc gatgccggta cgctggtacg
     7321 tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc cagggcgtga tcctcatccg
     7381 tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt gctgaaccgg ttgacgagga
     7441 agatctggat accatcgacg gcagtgccgc ggaaggggac gatgaaatcg ctccggaagt
     7501 ggacgttgac gacgagccag aagaagaata attttacttc ttcatgccaa aagggagcca
     7561 tctccctttt ttgaattgaa aagtccaggc tgcaaagtct gggcttttgt cgtattaggg
     7621 cgcggtaaag tttggctgtg tccgtaaaaa atggctggct atacacaagg aatgtggcaa
     7681 tgagtggtga aaaaaagcga aaggctggcg gttctatggt cttgtaggtt ttggcgcaat
     7741 agcactgctt tccgctggcg tctgggcgtt gcaatatgct ggcagtgggc cagaaaaaac
     7801 gttgtcgccg ctggtggtgc acaataattt gcaaattgat ctcaatgagc cggacctctt
     7861 tctcgacagc gattctctaa gccagcttcc caaagatctc ctcaccattc cgtttctcca
     7921 cgatgttctg agtgaagatt tcgttttcta ttatcagaat catgccgatc gtctgggcat
     7981 tgaaggcagc attcgtcgca ttgtctatga acacgatctc acgctgaaag ataagctctt
     8041 ttcgtcactc ttagatcagc ccgcgcaggc ggcgctatgg cacgataaac aaggccatct
     8101 ttcacattac atggtgctga tccaacgcag tggtttaagc aaactgctgg agccattgtt
     8161 atttgccgct accagcgaca gccagttaag caaaacggaa atcagtagca tcaagataaa
     8221 tagtgaaact attcctgttt atcagttgcg ctataacggc aataacgccc tgatgttcgc
     8281 gacatatcag gacaagatgc tggtgttttc cagcacggat atgctgttta aagatgatca
     8341 gcaggatacc gaagccacgg cgatcgcaag tgatttgttg agcggcaaaa aacgctggca
     8401 agccagcttt ggcctggaag agcgtgctgc cgaaaaaacg ccagtacgcc agcgcatcgt
     8461 ggccagcgcc aggttgctgg gttttggcta ccagcggtta atgccttctt ttgctggcgt
     8521 acgcttcgaa atgggtaacg acggctggca cagttttgtg gcgttaaatg atgaatccgc
     8581 cagcgtagat gccagtttcg attttacgcc ggtctggaac agtatgcctg ccggggccag
     8641 cttctgtgtg gcggtgccgt attcacacgg tattgccgaa gagatgcttt cgcacatcag
     8701 ccaggaaaac gacaagttga atggggcgtt ggacggtgcc gcggggctgt gctggtatga
     8761 agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt gatggcactg ccgaacaggc
     8821 gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg cacgaaagca aagcgccaga
     8881 aggtgttttg ccggtaagcc agactcagca gggcgaagcg caaatctggc gtcgcgaagt
     8941 gagttcccga tacggtcagt atccgaaagc gcaggcggcg caacccgatc aattaatgtc
     9001 ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa acgctgcttt tctccctcga
     9061 tgacacgctg gttaataacg ccctgcaaac actgaataaa acccgcccgg caatggtgga
     9121 tgtaataccc actgatggca tcgttccgct ctatatcaat ccacaaggcg tggcgaaact
     9181 gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc gaaccggttt tttataacgc
     9241 cgcacaaact ttattaatgc cgaagctgga cgctttatct caacaaccgc gttatgtcat
     9301 gaagctggcc cagatggaac ccggtgccgc ctggcagtgg ctacccataa tctggcagcc
     9361 gctatgaggc acgggctgct ggcgctgatt tgctggctgt gttgtgttgt tgcccatagc
     9421 gaaatgctga atgtcgaaca atccgggctg tttcgcgcct ggtttgtacg cattgcacaa
     9481 gaacagctcc gccaggggcc aagtccacgc tggtatcagc aggattgtgc gggcctggtg
     9541 cgatttgcgg cgaacgagac gctgaaagtt cacgacagta aatggctaaa aagtaacggt
     9601 ttatctagcc agtatttgcc gccagagatg acgctaacac ctgaacagcg tcaactggcg
     9661 caaaactgga atcaggggaa cgggaaaacc ggcccctacg tgaccgcgat taatttgatt
     9721 cagtacaaca gccagtttat tggccaggac ataaaccagg cgctgcctgg cgatatgatt
     9781 tttttcgatc agggcgatgc ccagcactta atggtctgga tggggcgtta cgtcatctac
     9841 cacaccggaa gcgccacgaa aactgacaac ggaatgcgcg cagtcagtct gcaacaactt
     9901 atgacatgga aggacacccg atggataccc aacgattcca atcccaattt cattggcatt
     9961 tatcgtttaa attttctggc gcgatagcag cgtgcttgtc cctgtctctt gtgggaacag
    10021 ggcttgctaa tgctgatgat tcgcttcctt ccagtaacta tgcgccgccc gccgggggaa
    10081 cattcttttt gcttgctgac agcagtttta gcagcagtga agagtcgaaa gtgcgactgg
    10141 aagcgccggg gcgtgattat cggcgctatc agatggaaga gtacggcggc gtggacgttc
    10201 gcctgtatcg tattcctgac ccgatggcat ttttgcgcca gcagaaaaac ctgcatcgca
    10261 ttgtggtgca accgcaatat ctgggcgacg ggctgaacaa tacgctgacc tggctgtggg
    10321 ataactggta cggcaaatct cgccgcgtga tgcagcgtac tttctcttct cagtcacggc
    10381 agaatgtgac tcaggcatta cccgaattac agctcggcaa tgccattatt aaaccttccc
    10441 gttatgtaca gaacaaccag ttttccccgc tgaaaaaata tcccctggtg gaacagttcc
    10501 gttatccact atggcaggct aaaccggtcg agccgcagca aggggtaaaa ctggaaggcg
    10561 catccagcaa tttcatctcg ccgcagccgg gtaacattta tattcctctc ggccaacaag
    10621 agccgggact gtacctcgtc gaggcgatgg ttggtgggta tcgggcgacg acggtggtgt
    10681 ttgtttccga taccgtggcg cttagcaaag tgtcaggcaa agagcttctg gtgtggaccg
    10741 cgggtaaaaa acagggtgaa gcgaagcccg gctcagagat cttgtggact gacggtcttg
    10801 gcgtgatgac ccgcggtgtg accgatgaca gcggtacctt gcagttacaa catatatcgc
    10861 cagaacgttc atacattctg ggtaaggatg ctgaaggcgg cgtttttgtc tccgagaact
    10921 tcttctacga aagcgaaatc tacaacaccc gcttgtatat ttttaccgat cgcccgctat
    10981 atcgcgcagg cgatcgtgtc gatgttaaag tgatgggccg cgagttccac gatccgttgc
    11041 attcatcccc catcgtcagc gccccggcga agctttcggt gctggacgct aacggcagtc
    11101 tgttgcaaac cgtcgatgtc acgctggatg cgcgcaatgg cgggcaggga agtttccgcc
    11161 tgccagaaaa tgccgttgcc ggaggttatg agttacgtct tgcttaccgc aatcaggtct
    11221 atagcagcag ttttcgcgtg gcaaactaca tcaagccaca tttcgagatt ggtttagctc
    11281 tcgacaaaaa agagttcaaa actggcgaag cggtcagcgg caaactgcaa cttctctacc
    11341 cggatggcga gccggtaaaa aatgcccgcg tgcagttaag tttgcgcgct cagcaattat
    11401 caatggtcgg taacgatttg cgttatgccg gacgtttccc cgtgtcgctg gaaggcagcg
    11461 aaacggtgtc cgacgccagc ggtcatgtgg cgttaaatct ccccgccgcc gataaaccga
    11521 gccgctattt gttaaccgtc tccgccagtg acggcgcggc gtatcgcgtc accaccacca
    11581 aagagatcct cattgaacgc ggcctggcgc attactcttt aagtaccgcc gcacaataca
    11641 gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct ggaatcttca aaacaggttc
    11701 ctgttacgta tgaatggttg cgtctcgaag accgcacgag ccatagcgga gatctaccgt
    11761 caggcggcaa atcctttacc gtcaatttcg ataaacctgg caactacaat ctgacgttac
    11821 gcgataaaga cggcttaatt ctcgccgggt taagccatgc cgtcagcggt aagggcagta
    11881 tgtcgcatac tggtacggta gatatcgtgg cagataaaac gctgtaccag cctggcgaaa
    11941 ccgcgaagat gctgattacc ttcccggagc caattgatga agcattattg acgctggaac
    12001 gcgatcgcgt tgaacagcag tcgctgctat cgcatccggc aaactggctg acgttacaac
    12061 gtttaaacga tactcaatat gaagcccgtg ttccagtgag caattccttt gcgcctaaca
    12121 tcactttttc ggtgctgtat acccgtaacg gtcagtacag ttttcagaac gccgggatca
    12181 aagttgccgt tccccagctg gatatccggg tgaaaacgga caaaacccat taccagcctg
    12241 gtgaactggt caatgtcgaa ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc
    12301 taacggtagg cgtggtcgat gaaatgatct acgcgctgca accagaaatc gcgccgaata
    12361 tcggcaaatt tttctatccg ctggggcgta acaatgtgcg taccagctcc agtctgtcgt
    12421 ttatcagcta cgaccaggca ctctccagcg agccggttgc gcctggcgca actaaccgca
    12481 gcgagcggcg agtaaaaatg cttgaacgtc cacggcgtga agaggtggat accgcggcat
    12541 ggatgccgtc actcacaacc gataaacaag gcaaagcgta tttcacgttc ctgatgcctg
    12601 attcgttaac ccgctggcgt atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc
    12661 aggggcgtgc ttatctgcgt tcggaaaaaa atctctacat gaagtggagt atgccaacgg
    12721 tgtatcgcgt gggcgacaaa ccgtcggcag gactgtttat cttcagtcag caggataacg
    12781 aaccggtggc gctggtgact aaatttgcag gcgctgagat gcgccagacg ctgacgctgc
    12841 acaaaggggc gaattatatt tcgctggcgc agaacattca gcaatctggc ttgttaagcg
    12901 cagaactgca acaaaatggg caagtgcagg acagcattag cacaaaactg tcttttgtgg
    12961 ataacagctg gcccgttgaa cagcagaaaa atgtcatgct cggcggtggc gataacgcgc
    13021 tgatgttgcc cgagcaggcg agcaatatcc ggctacaaag tagtgaaacg ccgcaggaga
    13081 ttttccgcaa caatcttgat gcgttagtcg atgaaccgtg ggggggggtg atcaacaccg
    13141 gtagccgtct gatcccgctc agtctcgcct ggcgttcgct tgccgatcat caaagtgccg
    13201 ccgctaacga cattcgtcag atgattcagg ataaccgtct gcggctgatg caactggcgg
    13261 ggcccggagc gcgctttacc tggtggggtg aagatggcaa tggtgacgcc ttccttacgg
    13321 catgggcatg gtacgccgac tggcaggcca gccaggcgct cggcgtaacg caacaaccgg
    13381 aatactggca gcatatgctc gacagttatg ccgagcaggc agataacatg ccgttattgc
    13441 atcgggcgct ggtgctggcg tgggcacagg agatgaatct gccgtgcaaa acgttgttga
    13501 aagggttgga tgaagctatc gcccggcgcg gaactaaaac tgaagatttc tctgaggaag
    13561 acacccgcga tatcaatgac agcctgatcc tcgatacacc ggaatctcca ctggcagatg
    13621 cggtggcaaa cgtcttaacc atgacgttgc tgaaaaaagc gcagttgaag tccacggtga
    13681 tgccacaggt tcagcaatat gcgtgggata aagcggtaaa cagcaatcag ccgctggcac
    13741 acacggttgt gctgctcaat agcgggggcg acgctaccca ggcggctgct attttaagtg
    13801 gtttgaccgc tgagcaatct actattgagc gcgcgctggc catgaactgg ctggcgaaat
    13861 atatggcgac aatgccttcg gttgtgttgc ctgcgcctgc gggcgcatgg gccaaacata
    13921 agttaacttg agggggcgaa tactggcgtt gggttggtca gggcgtgccg gacattctct
    13981 cttttggtga cgaattatcg ccgcaaaatg tgcaggtccg ctggcgtgaa gcggcaaaaa
    14041 cggctcaaca aagtaacatt ccggtgaccg ttgaacgcca attgtatcgg cttatccctg
    14101 gtgaagaaga gatgagcttt actctgcaac cggtgaccag caatgagatt gacagcgatg
    14161 cgctgtatct cgatgaaatt acgcttacca gcgagcagga tgcagttctg cgctatggtc
    14221 aggtggaagt accgctcccg ccgggagccg acgttgagcg cacaacatgg ggcatttcag
    14281 tcaataaacc caacgctgga aaacagcagg ggcaattgct ggaaaaagcg cgaaatgaaa
    14341 tgggcgaact ggcctatatg gtgccggtga aagaactgac gggaacggtc actttccgcc
    14401 atttgctgcg cttctcgcaa aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt
    14461 cctatgcacc tgcacagcaa agtgttgcgg cagggagcga atggaccggg atgcaggtga
    14521 aataagtgaa ctggcgcaga atcgtctggc tattggcgtt ggttactctg ccaacgcttg
    14581 cggaggagac gccgttacaa ctggcgctgc gcggtgcgca gcacgatcaa ctgtatcagc
    14641 tgtcgtcatc aggcgtgacc aaagtctcag cattacctga cacgctcacc acgccgttag
    14701 ggagtttatg gaaactgtat gtttacgcct ggctggagga tacccatcaa ccggagcagc
    14761 cctaccagtg tcgcggaaac tccccggaag aggtttattg ttgtcaggct ggggagagca
    14821 taacgcgcga taccgcgctg gtacgttcct gtgggctcta ttttgcgccg cagcggttgc
    14881 atattggggc agatgtgtgg gggcaatact ggcaacagcg tcaggcacct gcgtggttag
    14941 cgtctctttc tacgctgaag ccagaaacct cggtaacggt taaatcgctg cttgattcat
    15001 tagcgacgtt acctgcgcaa aacaaagctc aggaagtctt actggatgtg gtgctggacg
    15061 aggcgaaaat cggtgtcgca tctatgctcg gtagtcgggt acgggtgaaa acctggagct
    15121 ggtttgccga tgataaacag gaaatccgtc agggtggttt tgctggctgg ttaaccgatg
    15181 gtacaccgct gtgggtgact ggcagtggca ccagcaaaac ggtactgacc cgttatgcga
    15241 cggtgcttaa tcgggttttg ccagtgccga cgcaagttgc cagtgggcaa tgtgtagagg
    15301 tggagctatt tgcgcgctag ccgctgaaga aaatcacagc ggagaagagt acgacggcag
    15361 taaaacccgg cgtacttaat gggcgctatc gtgtgacgtt tgccaatggt aaccacatta
    15421 cgtttgtcag ccacggtgag acgaccttac tgaccgaaaa gggcaaactg aaattgcagt
    15481 cgcatctcga tcgtgaagag tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc
    15541 cacctgaggc agcgaaagcc atgactgtgg cgatacgcac ttttttacag caaaacgcca
    15601 atcgggaagg cgattgcctg accatccccg acagcagcgc cacgcagcgt gtttctgctt
    15661 cgccagctac cacgggggcg cgaacgatga ccgtctggac gcaggatctc atctatgcgg
    15721 gcgatccggt tcattatcac ggcagtcgcg ctaccgaagg gacgctttcc tggcggcaag
    15781 ccatggccca ggcggggaag ggagagcgtt acgaccagat actcgcgttt gcttatcccg
    15841 acaacagcct tagccgctgg ggagcgccac gcacaacctg tcagttatta cccaaagcga
    15901 aagcctggct ggcgaaaaaa atgccgcagt ggcggcgtat attacaaggc gagacggggt
    15961 acaacgaacc agacgtgttt gcggtctgtc gtttagtctc aggtttcccc tataccgatc
    16021 ggcagcagaa acggctgttt attcgtaact tctttacgct tcaggatcgg ctggatttaa
    16081 cccatgagta tctgcacctg gcctttgatg gttatcccac cgggcttgat gagaactata
    16141 tcgaaacgct gacccgacaa ttattaatgg actaatgcta tgcgaaagat ttttcttccg
    16201 ttgttactgg tggcgctttc gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt
    16261 gacgcaccgc tctctggctg gcatccggca gagggcgaag atgccagttt ttcacaatcg
    16321 atcaattacc cggcgtcgtc cgtcaacatg gcagatgatc aaaatatctc agcgcagatc
    16381 cgcggcaaaa taaagaatta tgctgcggcg ggtaaagttc agcagggccg gctggtggtc
    16441 aacggtgcca gtatgccgca gagaattgaa tccgatggtt catttgcacg cccttatatt
    16501 ttcactgaag gcagcaacag cgtgcaggtc atcagcccgg atgggcaaag ccgacaaaaa
    16561 atgcagtttt actcaacgcc gggtacagga acgattcgtg cacgtttacg gctggttctc
    16621 tcgtgggata cggacaatac cgacctcgat cttcacgttg tcacgcccga tggcgaacac
    16681 gcctggtacg gtaacaccgt gctgaaaaac agtggtgcat tggatatgga tgtcacgacg
    16741 gggtacggac ccgagatttt cgccatgcca gcaccagttc acgggcgcta tcaggtgtat
    16801 atcaactact atggtgggcg cagcgaaacg gaattaacca ccgcccaact gacgctgatc
    16861 accgatgaag gatcggtcaa tgagaaacag gaaacattta ttgtaccgat gcgtaatgct
    16921 ggcgagctga cgctggtgaa aagttttgac tggtgatcca tcccttcaca caacctggct
    16981 ctttcttttt tcagataaga ctgctgggcg atctgccgac gccaggatat aaccattaac
    17041 aagcgtcggc agattggtca tttcttattt ttcgtactga gaactgtcca tttactccgg
    17101 ctttcctggt taaggcaatg gtttctaagc gatacgcatt attgcaatgg tgacagaaca
    17161 ttgcggtaac acgcttttac cgctacctta accacactcc atcggtcacc tgaggcggag
    17221 cttcgcccct ttgaaatacc ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat
    17281 gttcagagca ttggcgttag tgctctggct gttgattgct ttttcatccg ttttttacat
    17341 cgttaatgcg ttacatcagc gagaatcgga aattcgtcag gaatttaatc tgagttccga
    17401 tcaggctcag cgctttattc aacgcacctc tgatgtgatg aaagagctga agtacatcgc
    17461 cgaaaatcgc ttatcggcag aaaacggtgt gctttccccg cgtggacgag aaacgcaggc
    17521 ggatgtgcct gcgtttgaac cgctgtttgc cgactccgat tgttcagcaa tgagtaacac
    17581 ctggcgaggt tctctggagt cattggcgtg gtttatgcgc tactggcgcg ataatttttc
    17641 tgcggcttac gatctcaacc gggtattttt aatcggcagc gataacctct gcatggccaa
    17701 tttcggtctg cgtgatatgc cagtggaacg cgataccgcg ttgaaagctt tgcatgaacg
    17761 catcaataaa tatcgaaatg caccacaaga tgatagcggc agtaacctct actggatcag
    17821 cgaaggtccg cgccctggcg tcgggtattt ttacgcgttg acgccagttt atctggcgaa
    17881 ccggttgcag gcgcttttgg gtgtcgagca gaccatccgg atggagaact ttttcttacc
    17941 gggtacgttg ccgatggggg ttaccattct tgatgaaaat ggtcataccc tgatttcgct
    18001 taccgggcca gaaagcaaaa ttaaggccga tcctcgctgg atgcaggaac gctcctggtt
    18061 tggctatacg gaagggttcc gggagctggt gctgaagaaa aatctgccac cctcatcgct
    18121 cagcatcgtg tattcggtgc cggttgataa ggtgctggaa cgcattcgca tgttgatcct
    18181 taacgcaatt ttgctgaatg tgcttgccgg agctgcattg tttactctcg cgcggatgta
    18241 cgagcgacgt attttcattc cggcggaaag cgacgccctg cgactggaag aacatgagca
    18301 gttcaatcgc aagattgtcg cctccgcgcc agtgggtatc tgcattttgc gtaccgctga
    18361 tggcgtcaat attttaagta acgaactggc gcatacctat ctcaatatgc ttacgcatga
    18421 ggaccgccaa cgactgacac aaattatctg tgggcagcag gtcaattttg ttgatgtcct
    18481 gaccagcaac aataccaatc tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa
    18541 cgtggccatt tgtgtgctgg tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca
    18601 ggagatggca caagcagcgg aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt
    18661 cagtcatgag ctgcgaacgc cgctgtatgg cattatcggt aacctggatc tgttgcaaac
    18721 caaagagtta ccgaaaggcg tcgatcgtct ggtgacggca atgaacaact cttccagcct
    18781 gttgttgaaa attatcagcg atattctcga tttctcgaag attgaatcgg aacagttgat
    18841 gatcgaaccg cgtgagtttt caccgcgtga agtgatgaac cacatcaccg ccaactattt
    18901 accgctggtg gtacgcaagc agttaggctt gtactgcttt attgaaccgg atgtgccagt
    18961 ggccttaaat ggcgacccga tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc
    19021 cataaaattc accgataccg gctgtatagt tttgcatgtc cgcgctgatg gcgattatct
    19081 ctctatccgt gttcgcgata ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt
    19141 tgatcccttc ttccaggtcg gaacgggcgt acagcgaaac ttccagggga ccggtctggg
    19201 tctggcgatt tgtgaaaaac tgatcagcat gatggacggc gatatctcgg tagattcaga
    19261 accgggaatg ggcagccagt ttaccgtgcg tattccgttg tacggcgctc agtacccgca
    19321 gaaaaaaggc gtggaagggt tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc
    19381 gctctgtcag ttcctggaaa ccagtttgca gcgcagcggc atcgtcgtaa caacatacga
    19441 agggcaggaa ccgactcccg aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg
    19501 gcagggcaga gcggtagtga ccttctgtcg tcgacatatt ggtattccgc tggagaaagc
    19561 gccaggggag tgggtacaca gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg
    19621 tatttatttg atcgagatgg agagcgacga tcctgctaac gctctgccgt cgacggacaa
    19681 agcggtcagc gataatgacg atatgatgat tctggtcgtg gatgatcatc cgattaaccg
    19741 gcgtttgctg gcagatcagt tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg
    19801 cgtcgatgcg cttaatgtac ttagcaagaa tcatattgat atcgtgctta gcgacgtcaa
    19861 catgccaaat atggatggtt accgcttgac acaacgcatt cgtcagttgg gactgacgtt
    19921 gccggtaatc ggagtaacgg ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc
    19981 cggtatggac agctgcctgt cgaagccagt aacgctggat gttataaaac agacgctgac
    20041 ggtatatgcc gagagggtca ggaaatcgcg ggaatcgtag gacggataag gcgtttacgc
    20101 cgcatctggc attcagtgca aatgccagat gcgacgctga cgcgtcttat ctggcctact
    20161 ttaatgtaat ctgttgaaat aatgggaatc gtaggacgga taaggcgttt acgccgcatc
    20221 cggcaatcgg tgcaaatgcc agataagaca ctaacgcgtc ttatctggcc tacagatgat
    20281 tagtctttat ctgccggact taacgttact gaagagagat aattcagcag ggcgatatcg
    20341 ttctcgacac ccagcttcat catcgcagat ttcttctggc tactgatggt tttaatactg
    20401 cggttcagct ttttagcgat ctcggtcacc aggaagcctt ccgcaaacag gcgcagaact
    20461 tcactctctt ttggcgagag acgtttgtca ccgtaacctc cagcactgat tttttccaac
    20521 aggcgagaaa cgctttccgg ggtaaatttc tttccttttt gcagcgctgc gagggctttc
    20581 ggcagatcgg tcggtgcacc ttgtttcagc acgatccctt cgatatccag atccaatacc
    20641 gcactaagaa tcgccgggtt gttgttcatg gtcagaacaa taatcgacag gcttgggaaa
    20701 tggcgcttga tgtacttaat taaggtaatg ccatcgccgt acttatcgcc aggcatggag
    20761 agatcggtaa tcaacacatg cgcatccagt ttcggcaggt tgttgatcag tgctgtagag
    20821 tcttcaaatt cgccgacaac attcacccac tcaatttgct caagtgattt gcgaataccg
    20881 aacaagacta tcggatggtc atcggcaata attacgttca tattgttcat gtaataggct
    20941 accttgctac agcaagctct tgacataact gtcaatgtcg ctgatgtatt tttctattcc
    21001 tggaacatcc ttctcacgaa tcagatgttc cagcgtttca cataactgct tgccgggtac
    21061 cagatttagc atggcaaata cgcctttaag acgatgagcc gtttgggcta acgcagcaaa
    21121 gtcactggtt gctgcttcag tatacagcct cttaacatca tccggtactg tgtctacaaa
    21181 gagcgcataa tagccgctgg catggagttg cgcattttca tctccgccca gaggcgattc
    21241 tgtcacctct tcctgcgcca gttgcacttc aattaattgt aagaccgctt cctgcatagc
    21301 gttgctcata ttgaagttga cgcacaattg accaggccca atttcccgta cgccagactc
    21361 atcatcgctt aaaagcaagc cagaggcagt aagattagac ggattatccg ttaaaaagat
    21421 atcataatct tgactaatta atctttcatc gggtgtgata caggttgcac cccaattttc
    21481 taactggcga gtgacaatat tccgaatttc tgccgaagta acatccacca ttacgcagac
    21541 atcatccagt aaacgctctt cttcctcttc aacttccggg tcagctgcga gcattttgat
    21601 atgcacagag tagcgtgtac caagcccatc ccgcgttttg atgtttaaat gaccgcccag
    21661 tttacgtgcc agttgatcgc tcagccagaa tgccagcggg tccgccttgc catagcgatc
    21721 gttttgggtc tggttgataa acgggaagtg caaattatcc atttcatgaa tgcttacgcc
    21781 ttctccggtg tccagaatgc ggaacgtcag gcggtcttcg gaggactcat cctgatcaac
    21841 ctcaagggtg atttttccca attgcgttga ggtcacggca tattgcatca gtagcagcaa
    21901 aatacgtcgt aatgcatcgc gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt
    21961 aatcagcagt tgcagacctt tacgcttgat ggcaggcaac actgaaggca caacttcatc
    22021 aattaaatcc tgcacggaga acagcaccgt ctcacttttc cagctatcgt ccgcaagcat
    22081 gttcgctaac tgtatttcat cgaccaatcg caccagcaca tctgcctgat tcgccagttg
    22141 tttgctttcc ggggcgttga gtttagccgc gctctccgcc agggactgtg cgggttcttt
    22201 cagcgcatcg ccaatgtttt tcataaaggt catccgcccc tgctggtttt tctcatacag
    22261 acgctgcgcc tgcttgagtt tcttgtttac cagcacttcg cgatcctgat cgcgaataat
    22321 gaaaatttgt gtgcgcggcg caacctggct gcggaacatg cggatctcat acagctcgtt
    22381 attgatcgtc gcctgaataa tcccctgatg ctgttccgcc atggtggtga tgttttgcag
    22441 attcaaatgc ggcagcaaat gatcggcaat tttgttactt atgacagtgc ggttcgattc
    22501 ctgatcgtga accagcaggc cgagcggcag cagtgagact atctcttcat tgattgcccg
    22561 taaaatgcgc aattcgttat tgaccgccgt gttgggtaga ctttctgtac tgcgactgga
    22621 gaaatggcgg aatgtggtat agccaaataa cgccagcgcc agcaaaccga tgttcagcag
    22681 cagtggcagc agaatgtttt gcaacgtatc cagcaataag gtgccataag gaacctgcca
    22741 gaccaggcgc atatcggtag agttgagtgc cgaggagatt tcaatcttcg tactgttaaa
    22801 gtggatactg acgctatccg ttccttcttt ctcattatca ttgtttcccg ttgccgtcgc
    22861 gtctggctca aggcggaaac tgtccagcgg catacccggt gggatcaaat cattaatcgg
    22921 cagatcaaaa gccacgaccg ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt
    22981 aaagtaatga ccgttctgcc aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc
    23041 gagggcgttg gcctgttgca acatctctgc acgacgtgaa tcaacaatgt cactgacggt
    23101 cgattcttta aatccggagg tgagatcttt gaggggcagg gttgagatca gcaccagact
    23161 gttatcctga ccattcaggt aatacattga ccacggtaca ttttctgcgc cccacaatgt
    23221 gtccagatag gtggacatcc gttgagtcat ctcaagcgtt gagttgtcgt gagagccaaa
    23281 gattaacgct tccgttttgc ggcgcggttt ttccagatag tagacatcct gtttcaggcg
    23341 cgtctcttgt aaaccttcgc cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat
    23401 ctgccaggtc acgtaacgcc aggtatcgac gcgcttttgg atagcgtggg taatgtcgac
    23461 aatttggtaa cttttatctt tcagccaggc gttaacggcg ctttgtacca ttacacccat
    23521 cgtcaccagt aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag
    23581 tgaaaagcgg gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa
    23641 acttcgcgaa gtgatagggc agtataaagg gtacagtgtg aaattccaga ctcttacctg
    23701 attgatgatt ctctattctc ccgtgatatt tatcatcctg cattggggga aatagatgta
    23761 caagcgccat tttgctgtac aaataaccta caaaaagccc aacgaaaata attgcaaata
    23821 aacaaagatt gctggaaatt atgctgatgt taattatttg tgaaatagtt aacaagcgtt
    23881 atagtttttc tgtggtagca cagaataatg aaaagtgtgt aaagaagggt aaaaaaaacc
    23941 ggatgcgagg catccggttg aaataggggt aaacagacat tcagaaatga atgacggtaa
    24001 taaataaagt taatgatgat agcggtagtt attctagttg cgagtgaagg ttttgttttg
    24061 acattcagtg ctgtcaaata cttaagaata agttattgat tttaaccttg aattattatt
    24121 gcttgatgtt aggtgcttat ttcgccattc cgcaataatc ttaaaaagtt ccctcgaatt
    24181 tacattttga aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc
    24241 gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta
    24301 atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg
    24361 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct
    24421 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg
    24481 cactatttct ctgacgacaa gtctgtagat ggcgaccaga cctacatgcg tcttggcttc
    24541 aaaggtgaaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc
    24601 cagggcaaca gcgctgaaaa cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg
    24661 aaattccagg atgtaggttc tttcgactac ggtcgtaact acggcgtagt ttacgacgta
    24721 acttcctgga ccgacgttct gccagaattc ggtggtgaca cctacggttc tgacaacttc
    24781 atgcagcagc gtggtaacgg tttcgcgacc taccgtaaca ctgacttctt cggtctggtt
    24841 gacggcctga actttgctgt tcagtaccag ggcaaaaacg gtagcgtaag cggcgaaggc
    24901 atgactaaca acggtcgtga agcactgcgt cagaacggcg acggcgttgg cggatctatc
    24961 acttatgatt acgaaggctt cggtatcggt gctgcagttt ccagctccaa acgtactgat
    25021 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta cactggtggt
    25081 ctgaaatacg acgctaacaa catctatctg gctgctcagt acacccagac ctacaacgca
    25141 actcgcgtag gttccctggg ttgggcgaac aaagcacaga acttcgaagc tgttgctcag
    25201 taccagttcg acttcggtct gcgtccgtcc ctggcttacc tgcagtctaa aggtaaaaac
    25261 ctgggtgtca tcaatggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
    25321 ggcgcgacct actacttcaa caaaaacatg tccacctatg ttgactacaa aatcaacctg
    25381 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
    25441 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
    25501 ttcattgttt tcagcgtaca aactcagttt tttggtgtac tcttgcgacc gttcgcatga
    25561 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
    25621 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
    25681 cttgaaggca aaactatggg taccttctgg cgtgccagca tcccgggcat tgacgccaaa
    25741 cgcagcgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
    25801 tcgacctata aaaaagattc cgcgctgatg cgctttaacg actcgcaaag tttatcgccg
    25861 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctacgcat tggcgcgaag
    25921 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg tttcggtccg
    25981 gaacaacagc cggttcaaat tccgagccag gaacagatcg acgcgatgaa agccaaaact
    26041 ggtttacagc accttacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
    26101 ccggatttat atgtcgatct ctccaccgtc ggcgaaggtt atgcggcgga tcacctggca
    26161 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
    26221 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
    26281 aaagaaaacg cggttcaggc cgtggtggat atcaacggtc atgggattag cacctctggc
    26341 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
    26401 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgcacc gacggcgctg
    26461 gaagccgacg cctgggatac aggtttgatg gtactcgggc cggagaaagc caaagaagtt
    26521 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
    26581 tggatgtcac cacagtttaa aagcttcctt gtcagcgaaa aaaattaaag cgcaagattg
    26641 ttggtttttg cgtgatggtg gccgggcaga ctaaaggcta tccttaacca gggagctgat
    26701 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
    26761 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acaggcatct tttgccgtcc
    26821 gtcttgccgc gccagacatg ctttgcggga aaacgtctcc ttctacgcaa atgccagcga
    26881 ggcactcgcc gccggatttc gcccctgcaa acgctgccaa cctgataaag ccaatgccca
    26941 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
    27001 aacgttggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
    27061 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
    27121 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
    27181 cgacagcagc agttactacc acaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
    27241 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
    27301 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
    27361 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
    27421 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atactccgct
    27481 gacgttaccg ctggatattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
    27541 cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
    27601 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaattcc
    27661 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
    27721 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
    27781 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
    27841 gcttttaacg ctgcagagca actaatccgc gatattaatg acgttgccag ccagtcgccg
    27901 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
    27961 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
    28021 aataaaccgt ggcccgccat gccgcagagt tttcataatt tatgtcaacg tgcggctacg
    28081 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
    28141 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc aattgtttct
    28201 gtttctctgg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
    28261 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
    28321 tatcacggta ttcaaccgtt gaaagcgggg tttcatccac tcaccaccga ctgccgctac
    28381 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
    28441 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
    28501 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
    28561 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
    28621 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
    28681 gtcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
    28741 cgtagtgaat ttatcaagcg gattctggat actcacgtcg agcgcattga acaactcggt
    28801 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
    28861 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
    28921 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcagtcac catctggggc
    28981 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagac
    29041 aagctgtaca cggattttca aactgtactt gaagggcgca aagagctgac tctgaaccgg
    29101 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
    29161 catattattc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
    29221 ctgggcgcta tcggcctggt gttctggatg gcgaacagtc tcggttgggc tgataccaac
    29281 gttgccgcga cctattcgtt gacgctttta ttcctgcgta cgccgctgct ttcggcggtt
    29341 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
    29401 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccagctg gcaaacgctg
    29461 gagctgcgta acgtgacgtt tgcttatcag gataacgcgt tttccgttgg tccgattaat
    29521 ctcaccatca aacgtggcga gctgctgttt ctgattggcg gcaacggtag cggaaaatcg
    29581 acgctggcga tgttgttgac gggcttgtat cagccacaaa gcggcgaaat cttgctggat
    29641 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
    29701 accgatgtct ggctgtttga tcaactgctg gggccggagg gtaaacccgc taacccgcaa
    29761 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagaaacggg
    29821 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
    29881 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
    29941 ttccgtcgtg agttttatca ggtgttgctg tcgctgatgc aggagatggg taaaactatt
    30001 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
    30061 aacgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
    30121 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatg
    30181 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
    30241 aaattagaat ataatttata aacattattt aaatgttgtt acttaagtgt taccgttgat
    30301 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
    30361 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
    30421 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
    30481 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
    30541 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aataacgccg gaaccgggca
    30601 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagca tcagtattga
    30661 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
    30721 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
    30781 tgtctggggc gaggataacg tcaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
    30841 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
    30901 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
    30961 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
    31021 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
    31081 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacatcc gtgcgaaatt
    31141 tgtctttatc ggcgcgggcg gtgcggcact gaagctgtta caggaatcgg ggattccgga
    31201 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
    31261 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
    31321 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
    31381 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
    31441 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
    31501 gaaatatctg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
    31561 gtactatccg caagcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
    31621 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
    31681 tgaccagcaa ggaaccattg ccgcgctcct gggggcatcg ccaggggcgt caaccgccgc
    31741 gccgattatg ttgaatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
    31801 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aggctgaacg gtgatgtagc
    31861 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg gggctgaaat acgacaaacc
    31921 gcaagcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
    31981 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtat
    32041 gctgataaga cgcgccagcg tcgcatcagg caaccggctc gggcgttaga tgtcggatgc
    32101 ggcgtgaccg ctttatccaa cctacggggt gccgcacttc acggcagttt agcgaactac
    32161 cgcgttgtca attttctctt ccgccttcca gacgcggtac ttcacatcta cattgtctgg
    32221 cgtatacacc acgatcggca gcttgctgtt gtaacgcagc attccagtat cgcccagata
    32281 cgcggtgaca aatttcttct ctttcttgcc atccgggcag gccatcatcg tggaaaccgg
    32341 cgagctaact ttatcaaaga cataataatc atagccccag ccttccagcg ttttgctttc
    32401 cagcttcccg ccgagacgat gcaaattgca atcgacttcc agcgtctgac cgattaacag
    32461 ttctactttc agggtagatt catcttcttg cggggttaac tgaatcacct gacgcttcat
    32521 gcctttttca gcttgtggat aaggcgcgat tttttccagt ggctggacgc tctctgccgc
    32581 ccaggcggaa gtggtggcga aagcggcaaa caatactgca ggtagaatgg tcttcatttt
    32641 ttgctttcct tcttcattta taggtttgca ccgatgatgt cgctgaaggt taacatattc
//
LOCUS       DAZFTN010000052        32306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFTN010000052 DAZFTN010000000
VERSION     DAZFTN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(98..1144)
                     /gene="aroH"
                     /locus_tag="ACHKV2_002590"
     CDS             complement(98..1144)
                     /gene="aroH"
                     /locus_tag="ACHKV2_002590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO6940455.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1301..2134)
                     /gene="ppsR"
                     /locus_tag="ACHKV2_002591"
     CDS             complement(1301..2134)
                     /gene="ppsR"
                     /locus_tag="ACHKV2_002591"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO6940456.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2467..4845
                     /gene="ppsA"
                     /locus_tag="ACHKV2_002592"
     CDS             2467..4845
                     /gene="ppsA"
                     /locus_tag="ACHKV2_002592"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO6940457.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4902..6548)
                     /gene="fadK"
                     /locus_tag="ACHKV2_002593"
     CDS             complement(4902..6548)
                     /gene="fadK"
                     /locus_tag="ACHKV2_002593"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO6940458.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6605..6898)
                     /locus_tag="ACHKV2_002594"
     CDS             complement(6605..6898)
                     /locus_tag="ACHKV2_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6940459.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6895..8184)
                     /locus_tag="ACHKV2_002595"
     CDS             complement(6895..8184)
                     /locus_tag="ACHKV2_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6940460.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8240..9178)
                     /locus_tag="ACHKV2_002596"
     CDS             complement(8240..9178)
                     /locus_tag="ACHKV2_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO6940461.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9198..9962)
                     /locus_tag="ACHKV2_002597"
     CDS             complement(9198..9962)
                     /locus_tag="ACHKV2_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO6940462.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10278..11189
                     /locus_tag="ACHKV2_002598"
     CDS             10278..11189
                     /locus_tag="ACHKV2_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6940463.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(11232..12383)
                     /locus_tag="ACHKV2_002599"
     CDS             complement(11232..12383)
                     /locus_tag="ACHKV2_002599"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO6940464.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12397..13992)
                     /locus_tag="ACHKV2_002600"
     CDS             complement(12397..13992)
                     /locus_tag="ACHKV2_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO6940465.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(14137..14895)
                     /gene="aroD"
                     /locus_tag="ACHKV2_002601"
     CDS             complement(14137..14895)
                     /gene="aroD"
                     /locus_tag="ACHKV2_002601"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO6940466.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(14926..15792)
                     /gene="ydiB"
                     /locus_tag="ACHKV2_002602"
     CDS             complement(14926..15792)
                     /gene="ydiB"
                     /locus_tag="ACHKV2_002602"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO6940467.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(15804..17069)
                     /locus_tag="ACHKV2_002603"
     CDS             complement(15804..17069)
                     /locus_tag="ACHKV2_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940468.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(17296..18510)
                     /locus_tag="ACHKV2_002604"
     CDS             complement(17296..18510)
                     /locus_tag="ACHKV2_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940469.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(18610..18966)
                     /locus_tag="ACHKV2_002605"
     CDS             complement(18610..18966)
                     /locus_tag="ACHKV2_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO6940470.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(19102..19209)
                     /gene="rprA"
                     /locus_tag="ACHKV2_002606"
     ncRNA           complement(19102..19209)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKV2_002606"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(19395..20507)
                     /gene="ydiK"
                     /locus_tag="ACHKV2_002607"
     CDS             complement(19395..20507)
                     /gene="ydiK"
                     /locus_tag="ACHKV2_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO6940471.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            20896..23952
                     /locus_tag="ACHKV2_002608"
     CDS             20896..23952
                     /locus_tag="ACHKV2_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO6940472.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            23949..24359
                     /gene="menI"
                     /locus_tag="ACHKV2_002609"
     CDS             23949..24359
                     /gene="menI"
                     /locus_tag="ACHKV2_002609"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO6940473.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            24459..24647
                     /locus_tag="ACHKV2_002610"
     CDS             24459..24647
                     /locus_tag="ACHKV2_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO6940474.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            25196..25564
                     /gene="sufA"
                     /locus_tag="ACHKV2_002611"
     CDS             25196..25564
                     /gene="sufA"
                     /locus_tag="ACHKV2_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO6940475.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            25573..27060
                     /gene="sufB"
                     /locus_tag="ACHKV2_002612"
     CDS             25573..27060
                     /gene="sufB"
                     /locus_tag="ACHKV2_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO6940476.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            27070..27816
                     /gene="sufC"
                     /locus_tag="ACHKV2_002613"
     CDS             27070..27816
                     /gene="sufC"
                     /locus_tag="ACHKV2_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO6940477.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            27791..29062
                     /gene="sufD"
                     /locus_tag="ACHKV2_002614"
     CDS             27791..29062
                     /gene="sufD"
                     /locus_tag="ACHKV2_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO6940478.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            29059..30279
                     /gene="sufS"
                     /locus_tag="ACHKV2_002615"
     CDS             29059..30279
                     /gene="sufS"
                     /locus_tag="ACHKV2_002615"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO6940479.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            30292..30708
                     /gene="sufE"
                     /locus_tag="ACHKV2_002616"
     CDS             30292..30708
                     /gene="sufE"
                     /locus_tag="ACHKV2_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO6940480.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            30857..31861
                     /gene="ldtE"
                     /locus_tag="ACHKV2_002617"
     CDS             30857..31861
                     /gene="ldtE"
                     /locus_tag="ACHKV2_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO6940481.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(31924..32160)
                     /gene="lpp"
                     /locus_tag="ACHKV2_002618"
     CDS             complement(31924..32160)
                     /gene="lpp"
                     /locus_tag="ACHKV2_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO6940482.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         8211 a         7963 c         8214 g         7918 t
ORIGIN      
        1 tattatcaat attatggctg accagagcaa gcagtaatgt gttgagaaga caacaagttg
       61 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcag
      121 aggctagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
      181 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
      241 tttccgccat aattccagca atcgccgtag agccattgcg gatttgctga caaatatcct
      301 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
      361 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
      421 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
      481 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
      541 ctgcgcggat agcatccaca gcaatccgcg tattgccatc ggtaccattt ttaaaaccta
      601 ccggacagga gagtgccgaa gccatttcac ggtggatctg actttcggta gtacgtgcgc
      661 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
      721 cagtcgcggt tgggacgccc agctcattta cctgtaaaag taatttgcgc gccagctcca
      781 gaccgtgatt tatccgatag ctgccgttta aatctggatc ggagattagt cctttccagc
      841 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
      901 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
      961 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattgagt attttttcaa
     1021 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
     1081 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
     1141 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctgccgggaa gtgatgcgca
     1201 tcacgataaa ccaatcgctg ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
     1261 ttagcatgat aacaaaaaaa taagctaatg cactagttct ctagtacatt cggcgactaa
     1321 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
     1381 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
     1441 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
     1501 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
     1561 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
     1621 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
     1681 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
     1741 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
     1801 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
     1861 tatcctggca aaaaccttca ctttgcaaga tgatggcgcg aatttccggc aacacgatgg
     1921 agtagaagac cagcggacgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
     1981 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
     2041 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
     2101 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga tgcagacata
     2161 attctatgaa agcataaatt aaaaacgcac agaagcgtag aacgttatgt ctggtttata
     2221 aaatgaacct tcaattttat tttttatgaa aacagcattt catttttatg gtttcgttta
     2281 taccgatggt ttatgtggaa attgtcgaaa agagcagatt tgcgcaacgc tgggatcagt
     2341 cttaacaagt aaaaaaatat atttgcttga acgattcacc gtttttttca tccggttaaa
     2401 tatgcaaaga taaatgcgca gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat
     2461 tgttcgatgt ccaacaatgg ctcgtcaccg ctggtgcttt ggtataacca actcggcatg
     2521 aaggatgtag acagggttgg gggcaaaaat gcctccctgg gtgaaatgat tactaacctt
     2581 tccggaatgg gtgtttccgt tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag
     2641 tttctggacc aaagcggcgt aaaccagcgc atttatgaac tgctggataa aacggatatt
     2701 gacgatgtta ctcagcttgc gaaagcgggc gcgcaaatcc gccagtggat tatcgacact
     2761 cccttccagc ctgagctgga aaacgccatc cgcgaagcct atgcacagct ttccgccgat
     2821 gacgaaaacg cctcttttgc ggtgcgctcc tccgccaccg cagaagatat gccggacgct
     2881 tcttttgccg gtcagcagga aaccttcctc aacgttcagg gttttgacgc cgttctcgtg
     2941 gcagtgaaac atgtatttgc ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac
     3001 cagggttacg atcaccgtgg tgtggcgctc tccgccggtg ttcaacggat ggtgcgctct
     3061 gacctcgcat cttctggcgt gatgttctcc attgataccg aatccggctt tgaccaggtg
     3121 gtgtttatca cttccgcatg gggccttggt gagatggtcg tgcagggtgc ggttaacccg
     3181 gatgagtttt acgtgcataa accgacactg gcggcgaatc gcccggctat cgtgcgccgc
     3241 accatggggt cgaaaaaaat ccgcatggtt tacgcgccga cccaggagca tggcaagcag
     3301 gttaaaatcg aagacgtacc gcaggaacag cgtgacatct tctcgctgac caacgaagaa
     3361 gtgcaggaac tggcaaaaca ggccgtacaa attgagaaac actacggtcg cccgatggat
     3421 attgagtggg cgaaagatgg ccacactggt aaactgttca ttgtgcaggc gcgtccggaa
     3481 accgtgcgct cacgcggtca ggtcatggag cgttatacgc tgcattcaca gggtaagatt
     3541 atcgccgaag gccgtgctat cggtcatcgc atcggtgcgg gtccggtgaa agtcatccat
     3601 gatatcagcg aaatgaaccg catcgaacct ggtgacgtgc tggtcactga catgaccgac
     3661 ccggactggg aaccgatcat gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt
     3721 acctgtcacg cggcgatcat cgctcgtgaa ctgggcattc cggcggtagt gggctgtggt
     3781 gatgcaacag aacggatgaa agacggtgag aacgtcactg tttcttgtgc cgaaggtgat
     3841 accggttacg tctatgcgga gttgctggaa tttagcgtga aaagctccag cgtagaaacg
     3901 atgccggatc tgccgttgaa agtgatgatg aacgtcggta acccggaccg tgctttcgac
     3961 ttcgcctgcc tgccgaacga aggcgtgggc cttgcacgtc tggaatttat catcaaccgt
     4021 atgattggcg tccacccacg cgcactgctt gagtttgacg atcaggaacc gcagttgcaa
     4081 aacgaaatcc gcgagatgat gaaaggtttt gattctccgc gtgaatttta cgttggtcgt
     4141 ctgactgaag ggatcgcgac gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt
     4201 ctctctgatt ttaaatcgaa cgaatatgcc aacctggtcg gtggtgagcg ttatgagcca
     4261 gatgaagaga acccgatgct cggcttccgt ggcgcgggac gctatgtttc cgacagcttc
     4321 cgcgactgtt tcgcgctgga gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg
     4381 accaacgttg agatcatgat cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt
     4441 gaggaactgg cgcgtcaggg gctgaagcgt ggtgagaacg ggctgaaaat catcatgatg
     4501 tgtgaaatcc cgtccaacgc cttgctggcc gagcagttcc tcgaatattt cgacggcttc
     4561 tcaattggct caaacgacat gacgcagctg gcgctcggtc tggatcgtga ctccggcgtg
     4621 gtgtctgaac tgttcgatga gcgcaacgat gcggtgaaag cactgctgtc gatggcgatt
     4681 cgtgccgcga agaaacaggg caaatatgtc gggatttgcg gtcagggtcc gtccgaccac
     4741 gaagactttg ccgcatggtt gatggaagag gggatcgata gcctgtctct gaacccggac
     4801 accgtggtgc aaacctggtt aagcctggct gaactgaaga aataaaataa atccccggcg
     4861 gcgttttgtc gccgggttta tgtgatcccc gaagatgaaa cttattcaat ctcttcacag
     4921 acatcctgcg ttaatcgccg cataatatct ttccgtaaca aaaacttttg tattttacct
     4981 gaggcagtgc gcggtagttt ttcgattacc acgatatgtt caggatattt atattttgcg
     5041 acccgtttac ggctaaaaaa agccaccacc tcttccagcg ataatgaatg atgcggcgct
     5101 ttcagcacga cataagcgca tgatcgttca cctaaacgtt catcgggcat tgcaaccaca
     5161 caggcatcgt gaattttagg atgctgcaat aaaatatctt ccacttcacg gctgctaata
     5221 ttttcgccgc cgcggacaat aatatctttt ttgcgtccgg taatttttat atagccagcc
     5281 tcatccatac ggcagagatc gccgctgtaa taccagcctt cttcatccag ggcacgggcg
     5341 gttaattcag gttcatcaaa atatcccata aacacattgg ggccacgcga ggcttcttca
     5401 ccttcgcaac ctggcggtaa ggtcttgcgt gcgtcatcga ccactttaat ctctacacct
     5461 gcggcagcgt aaccatcggt gtgcataaag cgcgacaaag gatcatcgag attcaccacc
     5521 gcatgcggcg aactttctgt ggaaccataa acacttaata atttaatgcc gcgctgctgg
     5581 cattcacgcg ccactttttt ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct
     5641 gaaaggtctg cgggttgttt ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg
     5701 ccgagcatac aggagcaacg ctgctgctca agcagcgcga gacacgcatc aggagtgaaa
     5761 atatctaaca acacgctgcg agcgccaatt aagaatggtg ccgttacgcc atgcagaaag
     5821 cccgttgcgt gaccaagtgg cgcaggcatc ataaagacat cctgccaggt tagattcagc
     5881 cgcgcgcaat aagcccgctc actggcgaga atattgttat gcgtaagcat cacgcccttt
     5941 ggcagaccct cggttccgga ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt
     6001 atcgccgtgg tcagtggggt attgtcggcg ataatctgac ttaatgagag ggaagaggtg
     6061 gcgggagcca gtttgtccac gccgacaatt tgttgtagtt gtggaagctg attttgcagc
     6121 ggcaggatta aatctaccgg acgcgtttgt ttaaacaacg tcggtgtaaa gaacattttt
     6181 gcctgacact tattaagcac ccataccagt tctgcttccc gccaggaagg caacagcggt
     6241 acggaaaccg caccgatttt caggcaggca agatagataa cggtaaattc acaccagcct
     6301 ggcagttgaa atgctatgcg atcgcctgat tcaataccct tcgctaacat ccagtttgcc
     6361 agacagctcg cggcgtgatc gagcgcgcta taggtgtacg atgctccatg attatcgacc
     6421 acggcaattt tgtctggcat cgcacgagcg gtctgctgcc agtaatcggc cagcgaagca
     6481 tcgccccata gcccttgctg acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc
     6541 actttcattt tggactctcg aaacagaaca tcaggcccga gatgcgggcc tgtgggatgc
     6601 atgattagcc gtagcgaaaa tcaataccga aggtgcctgc gggatattgc cactgttcga
     6661 gaatagtgtt accgcacagc acccgacagg tgccgcactc cagacaaccg gcggaatcaa
     6721 aatgaatgtt tcctgcgtca tcctgcttat aaagtccggc agggcaggct ttcattaatt
     6781 tacggaattc attgatatcg ggatttgccg ccaaaatgat atgcgggtgg ccctcatcaa
     6841 catggaattt attgacgcct aatttgatgt cgacgttaac cgtagcgttc tggctcatag
     6901 cgcggttgct cccttaatgc catctttcag caagttgatc agcccaattt tcttcgcgtg
     6961 tcccatgatc attttgcgta ccggctggtt aggcttaccg tcaatggtga acatctcgtt
     7021 catgatgtcg gctaccattc gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc
     7081 cgggatcttg cgaaaatgct gcatatcacg catgacgcag ctttgttcca gctcacgttt
     7141 gtattgcgcc agactgctgg cggagaaatc cgcgcgttct ttggcggcga tcactgttgt
     7201 ggcggcagcc tgagccgatg caatggctaa atccatgccg cggaccgtaa aacccaaatt
     7261 caggcagaag cctgcggcgt caccaacgat catcacgcca tcgttaacca gttgcggtac
     7321 catcgccaga ccgccttctg gcaccatatg cgcggaatat tcaagcagtt tgccgccgct
     7381 aatcagcggg cgaatggcgg ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt
     7441 ttgcgcatgg gcgatatcag ccaatccaca aaccagcccc aaggatatgg aatccttgtt
     7501 ggtatagaga aatcccccgc ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc
     7561 ggcaccttcc tcgcccgtaa tattaaagcg atcgttgatc tgttctggtg tgaggccaat
     7621 aacctcttta acaccaacag cgtaatgatg cggatcggaa gcgggaacca ttcccagcga
     7681 gcggccaagc atcgagttaa cgccatcggc taaaatcacc acattggctt cgagaatatc
     7741 atccccggcc tggactccag tgaccttgtt tccttcacga accaacgcat cgacgcgaac
     7801 tcccgggata aactgtgcgc ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg
     7861 attacgcaat acggtataag atgcgtgttg cggaacatct ggttgctcgc ggtgaaaatc
     7921 gagggtaacg gcgctctctt cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc
     7981 taccggcgct gatgctgcaa agcctggaat gattgcttca agtgtgtggg cataaagacg
     8041 cccgccggtc atgtttttac atccggcact gtcgccgcgt tctatcacca ggatatccag
     8101 cccggctcgc gccatgacca gtgcggcaac gctaccagca acgcccgcac cgaccacaat
     8161 ggcatcaaat ttgtcatccg acatgcgtta tgctcccttc ccgctgcgtt tacagcggga
     8221 gatgtggttg aaaggggaat tagcggctca actggctaat cagggcaggg acgactttgt
     8281 agatatcgcc caccagaccg tagtcggcat agttgaagat tggcgcattt ttatctttat
     8341 tgatggcgac aatcactttt gcgccgttgc cgccaaccat atgctggatc tgcccggaga
     8401 tccctagcgt caggtagaga tcggatttca gcaacacgcc ggagacaccg atataacgtt
     8461 cacgctccat ccagttctcg ccttcggcaa ttggacgtga acagccgact tcagcattca
     8521 gcaccgccgc cagttcgtgg accattttca ggtcatcctg cgctgccaga ccacgaccga
     8581 cgccaaccac acgttttgct ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt
     8641 cgcgacagag aatttcatga cgcggagcaa cgtaaggtac cgtttctgtc gggcactgat
     8701 gagaggtatc actggtgcac ggttcctgaa caccgggtgc aagggtaatg atcgccagcg
     8761 agctgttgat cttttcctga gcgaacgcta acccgccata catccggtgt tcggcgcaaa
     8821 tgtgaccatc gacaatatcc accgccgtgg catcgttcac cagcgccgca ttcagttgca
     8881 cacttaaccg tgctgccagc gctttaccac gtttcgtcgc ggccagtagc aacatagcgg
     8941 ggtgtttatc tttcagcaga gcagcaatgc tttcggcgta attttcagtg cgttgcagcg
     9001 cgtcgttttg ctcaagaaca taaatacatt ttggaccata aggcataact gcctgcgcct
     9061 ggtcggtatt ttgtacaatg gcatacacct gttggcccca ttgctgagcg ccgccaaaca
     9121 gttcagcata acgttcagga ttatcgctaa agacccagac gctgtttaat tgactcatcg
     9181 tttcttctcc gtaggcttca gttcagggct ttcttcagat gttccgccag ctcggcaatg
     9241 gcctccggcg aatcgttatc gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga
     9301 atgccggtca gttcagcaag tggtgcgctc tggctccagc caatatcact tgcctgccac
     9361 tgatttaccg gttttttacc cgcgccgaga atggctttca tcgaaggaat gcgtggcacg
     9421 ttaatatcgg aggtgacgca gagcacggct ggaacagaga gttcaataac ttcaacgtca
     9481 tcttcaagcg tgcgttcaat caccagtgta ttgccctgac gctgaatagc actcactgca
     9541 ttaatcaccg gaagttgcag aatttctccg accagcaagc caacctgctg ggcataaagg
     9601 tcgccggaac ctttaccaaa gatcagtaaa tcgaagccga tcttttcaac tgctgccgcc
     9661 agcgcttttg cggtatcgag aggcagtgca tgctcaagtt gtgcatcctg caccatatac
     9721 aggctgtgcg gcccgcggga tagcacgtct ttgcgcactt tcgagttctg caacaatgag
     9781 ccgccaacgg tcagcgcggc tatctcatcg tcatctgttg caagctggct tgcagcttca
     9841 atggcattga gatcgaactg gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat
     9901 tctggagtga caacaatgtc ctgttcttca ggcaccagct taaagcaggt tattattttc
     9961 atgatttctc ctgtgagtcg catacggaac catctccgat ggaaggtaaa aatggcctga
    10021 aaatagtgtg cgatagcggt gcggggtttt cgacggagca agctgttttc cttgccaaca
    10081 atcttgcaaa aaaaatgcga tcgctaaaac aaaagtgcaa tttgcggcaa tatagttatt
    10141 ccatgaccgt acaatcttgc attcagtgta aataatcttg tttagctctc gctaaacgcg
    10201 gttgtataac cgcaatacac gccgtatttt atccggcata tattgttttt gcttgctcat
    10261 ggaataggga ggttgtcatg tatcaacgct gttttgataa tgccagtgaa acgctgtttg
    10321 tcgctggtaa aacgccacgg ctttcacgtt ttgcatttag cgatgatcca aaatgggagt
    10381 ctggacatca cgttcatgac aatgaaaccg agctgattta cgtcaagaaa ggggtggcaa
    10441 ggtttaccat cgattcttcg ttatatgtcg cgcatgcaga tgatatagtg gtgatagaac
    10501 gcggcaggct gcatgcggtg gcctctgacg ttaacgatcc ggcaacgacg tgtacttgtg
    10561 cgctgtacgg ctttcagttt cagggggctg aggaaaatca tctactgcaa ccgcattctt
    10621 gtccggtaat tgccgcgggg cagggaaaag aagtcattaa aaccttattt aatgagctaa
    10681 gtgtgatttt gccgcaaagt aaaaatagcc aaacatcttc gttatgggat gcatttgcct
    10741 atacattagc aattctttac tacgaaaact ttaaaaatgc ttatcgttcg gagcaggggt
    10801 atattaaaaa agatgttctg ataaaagata ttcttttcta tctgaataat aattatcgcg
    10861 aaaaaatcac tttagaacag ttatcgaaaa aatttcgtgc cagcgtcagt tatatttgcc
    10921 atgaatttac caaagagtat cgtatttccc ctattaacta tgttattcaa cggcgtatga
    10981 cggaagcaaa atggtcactc actaatactg aattatcaca ggcagagatc tcctggcgtg
    11041 tgggttatga aaatgtcgat cactttgcca aactgttttt gcgccatgtc ggctgttcgc
    11101 ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga acaagaaatc ctatctgaat
    11161 ttcctcaacc ggtaagtctt gccggataaa tctggcggcg cagtttgtta agaagctgcg
    11221 ccgcctgcag attatttgtt ctgatagtct ttcaggatct gccgacctgc tacgtaaatc
    11281 ataatttcgt ctgtaccgcc gccaatacgt tcacaacgga catcacgcca gaagcgggag
    11341 acgcgcgcct catcggtata gcccagaccg cccatgattt gaatcgcatc atcaatgact
    11401 tccattgcgg tacgtgcaca gtacagtttt gccagcgccg cgctggtgcg cagtgattga
    11461 tgctgatcgg cttgccatgc cactttcagc accatgttgc gcatgttgtc aatcttaatc
    11521 gccatcagcg ccagtttttc ctggatcatc tggttatgac caatgggttt accaaaagcg
    11581 atacgctggt tggcatagcg agcggcatct tcaaaggcac actcggcaaa gccggtgctg
    11641 cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca tcacattgag gaaacccatt
    11701 ccttcttcgc ccaccatgtc gctctcttca acttccacgt tgtcgagata gacttcgcag
    11761 gtgctgagca tatgccagcc gattttatgc agcgggttaa tcttaatgcc gggcttactg
    11821 gagtcgaccc accacagggt gaaggctttt ttgggatctt tcggttgcgg atcgcgcgcc
    11881 agcaccagca tatacgggta ctctttcgca ccggtaataa aggttttctg tccgttgatg
    11941 taaaccttgc cgtttttacg cgtgtaagtg gtagtggcac tgttgttatc tgagccagca
    12001 cctggttccg tcaacgccag ggcataggcg ggatcacccg tttccagggt gctttctgcc
    12061 gttttacgta gctgctctgc agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg
    12121 gtaattaaaa atgccggagc accgcatttt gacacttcca tcagcgccag catttgggtg
    12181 acgtaatcag cagggatacc accaaattct tccggcacgc caagcatgga aataccgtta
    12241 tccgccagtg cccgcataaa ctcacgcgga tatgtcccgt tttgatcgca ggtgcggaaa
    12301 tactcttccg gaaagttagt cgtaatcagt tcgcgaatac tggccagcag cagttcttgt
    12361 tcttcagtta aagaaaaatc catcgtgata ctcctgttaa tgagccgctt caggcaggac
    12421 aaaacccatc gccgcatcga taaataatct ttcgtccatc agtttgagtt ctggcgaaat
    12481 cactggggtg aaatccattt tgtcgagaat atctttttgt aaatcgacgc caggggcgat
    12541 ttcgattaaa tgcagtccgt cttctttcag cgtgaatacg gcgcgctcag tgatataacg
    12601 aacatccagc cctcgctcga gagcgatttt tccgctaaaa gtaatttccg gtagttcccg
    12661 aataaatttc ttcacccgtc cttcctggac gatattcaat ttgccatcgg caatttctgt
    12721 ttttaaactg cccgcagtta acgtgccgca gaaaataatt ttcttcgagg tggcactgat
    12781 atcaataaat ccaccggtac ccatgatttt gccattgaat ttatgcacgc cgacgttacc
    12841 gtgctggtcg acttcagcaa aactcaaata acaaacatcc agaccaccac cgtgataaaa
    12901 atcaaactgg gacgtcatat ccagaatggc acgggtattc acgttcgcgc caaaggcgat
    12961 accctgtgaa gtaattccac caatcggacc tgtttctacc gtcagaataa agtcatcagc
    13021 acaaccttct tcccgggcga ccaggccaat gccgtcagca ataccgacgc cgacattccc
    13081 caccgcgcct ttacgcattt cgaataatgc acgccgtgca actaatttac gttggtttag
    13141 gggcaggcta agtttggtgc tgtcatcaag ggtgaagtca ccagaaataa agcggttaac
    13201 cggcgcaccg ccatatagtt ggctttgatc cggatcgacc accacaatat ccaccagata
    13261 acccggaata cggacagatt taggatgcag tgtggctttc ttaaccattt tctgcacctg
    13321 catcatcaca ataccgccgt tattgtgcac cgcctgggca ataaccaatg cgtcgagata
    13381 catcacctca tcttcaaaag tggcgtagcc ttcactgtcg caggtggtag cgcgaatgaa
    13441 agcaatatct ggcgcaattg ctttgtaata gagatattct ttgttatcaa actcgaccag
    13501 tttaatcagg tcttctttag tgacttcatt cagtttgccg ccttgctggc gtggatcgac
    13561 aaatgtcccg atgccaatat cactaataat accaggctgg tgggctgcgg cggcgcgtaa
    13621 ggtttgtgta agtacacctt gtggatagtt ataagcaata attttatttt gttctgcgag
    13681 atcagaaata cgcggcgatt gtccccagtg accgcataat gcccatttca ccagaccttc
    13741 ttgcgccagc ggactaatac cgcggtcggc gcgatcgcca agccctgttg gactaataat
    13801 cgataaatta cgtggcgttt gagtctgttt atatttatca gcaagagcag taattaacgt
    13861 ggtggcttcc agaataccgc cgccagcgcc taacacacaa agtgttgctt cgtcgggaat
    13921 ataattcacc gcttcctgtg ccgacaggac cggcactcgt ccattaatac gaggtggttt
    13981 tacaggtttc attgttaatt cctgattcag gtgtgatttt ccctacgcca gatggataat
    14041 tagcgcacag agactcacga aatattttta ttaaatacgg ttgaatatat ttttttagtt
    14101 cggcggggga ggatattccc gccgaaatat tattgcttat gcctgatgta aaatagttaa
    14161 tactgtgcgc aaatcattta ccgagatttg ccctggcgca gaggcttttt ttaccgcacc
    14221 aaaagttgcc gccgagccaa atacttcacc agccagacga gaaattacgc cagtttttgc
    14281 cattgacatc gtgatgattg gacgatcggc atactgctcc tgcatctcca gggtcgcggc
    14341 aagcaacgcc agcacatcgc tggtactttg cggcatcagc gcaatcttag gaatatcggc
    14401 gtcgaaggac tgcattttgc gcagacgggc aatgatttct tcggcttccg gcgttttatg
    14461 gaagtcatgg ttggacatga caactttcac atcatgcgcg tgggcgtagg cgacggtttc
    14521 tttaacctga tcatcgccgg taaataactc cagatcgatc atatcaacca ggccgctgtc
    14581 gatggctgca cgattgagag caatataagc ctcggtggaa atcgcctgct cgccgccttc
    14641 tttggcactg cggaaggtaa acagcagcgg tttttctggc atggtttcac ggagaatttt
    14701 tgccgccgcc atgacagact ccacattgga gaggtcggca aagtggtcca cacgccattc
    14761 cagaatatca aagtccgctt cacgataggc gagagcttcg gatttcactc gggcgatatc
    14821 tttcgccatc agcgagacga tgattttagg tgcgcccgta ccaatgacga gatcttttac
    14881 agttacggtt ttcatttttt accctttctg cacgcggtca gcctgtcagg caccgaaccc
    14941 catgacctgt ttaacatatt ccagagggaa atctttgcca gtccataacg tgaactgttc
    15001 agccccttgc cacaacaaca tgccgtatcc atcaatcgtt ttgcaaccag cttgttgcgc
    15061 ctgctgcaat aacttcgtca tatgcgggtt atacacgcat tcagtgacca gcagtcccgg
    15121 atgtaacaga ctgatatcat taaccaatga tttattctca aggggtttca tacccacttt
    15181 tgtgccattg gttaaaatgt cggcggaagc cagggcttca gcaaaggctt gctgatcggc
    15241 gagatcggta accgtgacga cacaatcggt gttttcgtta acccgctgcg cgaaggcgag
    15301 ggctttatcg aagaacttat cccgacggtt aaagagttta atttctttta aaccttcaat
    15361 tgccccctgc gcgccaatcg ccgttgaggc acctccggcc cctaacagca ccattgtttt
    15421 gcctttgata tcaaaaccgc tctctttaat ggcgcgaata tggcccgtgc cgtcggtgtt
    15481 atagccgcgc aggtagccat catcattaac gatggtgttg atggcaccca ccagtttggc
    15541 tgctggcgtt aattcatcaa catattcaca cgccagttgt ttgttcggca ttgatacacc
    15601 agttccgcgc atttttagag cttttaatcc ttcaattgct ccgggaaagc tatcgttatc
    15661 cacttcgaag gccatatagg taaatggcaa tcccgctttt tctaaggctt tattctgcat
    15721 ttcaggcgat aaactgtggc ggataggata agccatcaac ccaatcaatt cgtattttgc
    15781 ggtaacatcc ataattaact cctttaacct ctatgcttaa ttgtgtttaa ccgggtgcta
    15841 aatttgcgct cgccaaaccg cacatcattc tcaggaataa taaaaacgcg gtaatagcgg
    15901 ataaaaacaa taattgcggt aatcagggcc agcagcgcga aagtaaaatc gagaacaatg
    15961 atatattgca ggccgatgtt cgacagataa ccggtaatca gcggaataac aaagttagcc
    16021 agtccaccca tcatcatata aatactggtg actttggctt tgctttttgg gaaaaactct
    16081 gacatgaccg aaacaccgag ctgtaaaatg ccgccagctg ctgagaaacc gataacaaag
    16141 gcgccggcat tacacaccag tggagaaggg tacaggtaga taatggctgc tgttattgtt
    16201 gccagtgcag agttaaatac attagcccag atgggccgga ccattttttt cagtagtgcg
    16261 gcaaaaataa agacacagac cagcgagccc atactgtaat aagagatggt ttttaatgcc
    16321 tcagcttctg acataccagc aaaagccatc gcatatttgg gcatccacac cacaatcaca
    16381 taaaaggtcg agaatgcggc tacaccgaac agtaccgatg aaacaccttc cagccagact
    16441 aacggtttgc tgttcatttg cggtaattca ttagctacgc tggcgtccac caactggctg
    16501 gggaatttgc ttttcaacag catcagcgtg atcagtacaa acagaatacc cggaataatc
    16561 agcccatagc cgtaccagat attattgagc aacatatagc tcaccagcat tgggtagaac
    16621 atttgcccaa atgacaccat cgctttaacc agtatgaccg ccgaaccaga ggctttcgga
    16681 aagcattcca tgagcgcggg gtagccaccc gtatccagcg ctgagttagc gatacctacg
    16741 cacactgcca gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc aaagaagaat
    16801 agcagataca ttattactgc cattaatatc accgcccgac gaccaaactt atcggagatc
    16861 acaccgaaga ataaaatact gatcaatcgc cccaaaccga taccggaaat taagtaggca
    16921 atgcccgcgt tgtcagtgga aaacttttcc gccagagatg acatattttg ggcaagcgta
    16981 ataacactaa tgccgtgcag gaagtagctg aagtaaatac aaagaacagc caggataaat
    17041 ggcgtgctga aagccttatt ttgagacatt ttcacagctc cttgctgaaa gataatttcg
    17101 ttaccgtacg tattgttctc cgacggttta cataattact atactgaaga tgattttccc
    17161 tgggtacatg gcataaagtt tatgtttcca ggaatattgc atgtcatagt ttgtttttga
    17221 tcgcgatttt ccttagttag cgcagaaaga gttatttatc cgtaagatct tgcatgacct
    17281 gaaaagaata ttgcattacc cacccggagc gacattttcc tttagcgagt gatgccgcgt
    17341 ttttttgcgg ctgcgtaagc cgataaacag tgccagtaaa aaaccgatgg cagcgatggc
    17401 ggtatcgaac cacataatat cggcaatact tctttgggac agatgagccg taatcaacgg
    17461 aatagtaaag gtcgcaatac tgcccgcact gtaatagatc cctgtagctt tacctttagc
    17521 gtaagggaaa cgttcagcca ttaacgtcag gccaatttgc acaacacctc cagcagaagt
    17581 aaaaccaatg acaaaagaaa atattatcac cacataaaat gtgggatgca ggcagacggt
    17641 aaacagagca ataaatgaga taaaggtgta cagcatcagt aatgttgttg ggcgaacggt
    17701 attacgaatg agtggagcgg taataaatac acaaagcagc gaacccacgg tgtagatact
    17761 gagtagtttg atcgacatag tgtatgacat gcctgcaaca aattgtccgt actgtgccag
    17821 ccactggcta accagataaa acgttgccat tgagatatag ccatataagg tataactggc
    17881 taaatcgata attgaacagc gatgttccgt agagctggtg gttttcttta tgacaggtaa
    17941 gcgacgaccc ggatggggtg ggaacgtaca acggtataaa aacagagcgt taataaacat
    18001 aatgcctgca gcaatcataa aggaccaacc gaaccacagt tcagcccaca ctaacaggct
    18061 aatgattagc ggtaataaaa attgtccgct ggaaacaaat gctttaatta aaatattggc
    18121 tgtcccaggt gagcgtggaa aagcttccat caaactggga taagtgcctg catcgagaaa
    18181 actgtttgcc attcccgcca gaaagccaaa aacataagcg ataatgatgt tattggtctg
    18241 caggatgcca aaaaagaagg ccatatagca gcacatcccg agcatgataa aagggcggcg
    18301 accaaagcga tcggataata atcctgcaaa aagcaagaca cttaatcgac caatgcccag
    18361 cgatgagata actatcgaga cacccgcggc attagactgc caaagtgtct ccagcgaggc
    18421 catattcagg ctcatcaaaa tgacgcccat accatgcacc aggtaattaa aatacaaccc
    18481 aagtgcggta gggtaatagg gatttttcat aggttctact tagcaaaggt gctttaaaaa
    18541 gtgtagcgat gttgtgtcag tatccgttca ttattgttgt aacaacgaca attccttttt
    18601 gtggagaaat taacaaagcc gctccagatc gtgtgcatat aattcagcgg ctaccgattg
    18661 atagatcttc cagtcaataa aattgccatc agggtagact tgttgaaacg cggttaagtc
    18721 ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt attttctcta tgatcgcatg
    18781 aagatgtttt tttctttgct gacgcatagc caacagttgc tcgactacac aatcaggaat
    18841 ggcacacctg ccattttccc attgacgcca gctttcactg tcaccggtct gggcaatcca
    18901 ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg agtgcctgga gttcataagc
    18961 gttcatcgtg taatggctgc atgaaataaa caataaaata taaataacat tatcagcctg
    19021 ctgacggctt gaagagagtc acagtatctt gtgcaacatt attggaaaga gtgaggggcg
    19081 aggtagcgaa gcggaaaaat gttaaaaaaa agcccatcgt gggagatggg caaagactac
    19141 acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt
    19201 tataaccgtg agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt
    19261 agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga
    19321 aaatatgggg aagagcaagc aaatatcaat tagcggtggt taacaatcag gcggcagagc
    19381 cgcctgatga gaaattactt attcggtttc tggatttcgc caagttcttc gagaatttct
    19441 tccggttgat ccgtcggcgg tgggacctct tccacccacg cggcaaacag acgccaggaa
    19501 acggctaaca gaaccggacc aataaacaga ccgatcatcc cgaaagcaat caaaccacca
    19561 ataacgccag agaggatcag gatcagcggt aaatcggcac ccatgcgaat taacattggg
    19621 cggatgacgt tatccagcgt gccaaccaca ccgctccaca ctaacaatac cgttccccag
    19681 gtggtatcgc cagtccagta gagccagata atcgccggaa tcagtaccgg caacgggcca
    19741 agctggacaa ggcaggaaag gatcattaac accgttagca aagttgcata aggtacgccg
    19801 gatacggcga ggccgatacc gccaagcact gcctgtacta acgccgtcac caccacaccc
    19861 agcgccaccg cgcggatagc ctgtgccgcc agcagtacgg cggcatctcc gcgaacgcct
    19921 gccagacggg tcgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc
    19981 agggcactga agagaagcat cagcgcacaa tgcaccataa agcgcccgat atgcgccgct
    20041 tgcccaacga accaggtagt ggtggtgcca atataagggc ggactttcgc catgatcgcc
    20101 gtccccccca tatccagcaa gttgtgccag cctgcataca gcttcgcgcc aatcaccgga
    20161 atggtattaa gccacgccag atcgggtaac gtcatgtcac cgctggaaat ggctttaatt
    20221 agcgggccgc tgccgtcgac gatactgtta accagcaaag caatagggat gataaacacc
    20281 atcactaata acagcgtcat caccagaacg gcgagagagc ggcggccaaa catgatcttt
    20341 tgcaaacgta acaataccgg ccaggtggcg ataaccaccg taccggccca tgcaaagccg
    20401 agaataaagg gttgaacaat ccacagacat gccacaatca tgatggctaa aaacagcacc
    20461 gaaagcagaa tttgtgcgac atccctgggc tgacgaacat ttaccataac tacttttcac
    20521 cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc tgcataatga
    20581 tgagcaattt cagcggtttt taacaggccg attctgccct taattctgat gggcgtgtta
    20641 gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg atttactcgt cgtatttcaa
    20701 gttgcatatg ctgcggctac gttcgttcac cccagttact tactgttgta agctcctgga
    20761 gttcactcgc ttgccgcctt cctgcaactc gaaatccata gagtaaataa acatcacctt
    20821 tctgtgcgct attgcagccg cctgaaagaa cagtgtatcg acctacatca cagaccgcag
    20881 gaaagggtca atataatgat tccacagatt tcccaggcac ccggcgtcgt tcaactggtg
    20941 cttaattttt tgcaagagct ggagcaacaa ggttttaccg gcgatacggc gacaagttat
    21001 gctgatcgtc tgacaatgtc gaccgacaac agtatttacc aacttctccc cgatgcagtg
    21061 gtatttccgc gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc
    21121 tattcatcgc tgatctttac cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc
    21181 aaccagggta ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct
    21241 gaagagggct gggtgcgcgt tgaggccggg gtgataaaag accaactcaa tcagtacctg
    21301 aaaccgttcg gctacttttt tgcaccagaa ctttcgacca gcaaccgggc aacgctcggc
    21361 gggatgatca ataccgatgc atccggtcag ggatcgctgg tctatggcaa aacgtcagat
    21421 cacgtacttg gcctacgtgc ggtattgttg gggggcgata ttctcgatac gcaaccttta
    21481 cccgtcgagc tggcggaaac gctgggtaaa tccaatacca caatcgggcg aatttataac
    21541 acggtttatc aacgttgccg tcagcaacgc cagttaatca tcgacaactt ccccaaactt
    21601 aaccgctttc ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgagttc
    21661 gatctgacgc gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcgg
    21721 ctggatatta cgcgcttgcc taaagtgcgc cgtctggtga atgtcaaata tgactctttt
    21781 gactctgcgc tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg
    21841 gtggactcaa aagtgctgaa tctggcgcgg gaagatattg tctggcattc cgtcagcgag
    21901 ttgattaccg atgtgcctga caaagagatg ctcgggctga acattgtgga atttgctggt
    21961 gatgatgagg cgctgattga cgagcgggta aatgcactct gtgcgcggtt ggatgagctg
    22021 atcgccagtc atcaagcagg tgtcatcggc tggcagatgt gccgtgagct ggcgggggtt
    22081 gaacgtatct atgcaatgcg caaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc
    22141 gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc cggaacatct ggccgattat
    22201 attgctgaat ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac
    22261 gtcgacgcag gtgtcttgca tgtccgtccg gcactggata tgtgcgatcc gcaacaagag
    22321 attttgatga agcaaatctc tgatgacgtg gtggcgctga ctgcgaaata cggtggtttg
    22381 ttgtggggcg agcacggcaa aggttttcgc gctgaataca gcccggcgtt tttcggtgag
    22441 gaactttttg cagaactgcg caaagtgaaa gcggcatttg acccgcataa ccgactcaac
    22501 ccagggaaga tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg
    22561 aagcgcggta cattcgatcg gcagatcccc attgcggtac gccagcagtg gcgcggtgcg
    22621 atggagtgta acggcaacgg tttatgcttc aactttgatg cccgtagtcc gatgtgtccg
    22681 tcgatgaaga tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt
    22741 gaatggctgc gtttgttggc ggatcgcggc gttgatccac tcaaactgga acaagaactg
    22801 cctgaatccg gcgtcagctt gcggacgtta attgcccgca cgcgcaatag ctggcatgcg
    22861 aataaaggcg aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc
    22921 tgtaaagctt gttcgaccca gtgccccatc aaaattgatg tgccggagtt tcgctctcgt
    22981 tttctgcagc tctatcacac ccgttattta cgcccgctgc gcgaccacct cgtcgctacg
    23041 gtcgagagct acgcgccgct gatggcacgc gcgccgaaaa cctttaactt cttcattaac
    23101 cagccgctgg tgcgcaaact ctcggaaaaa catatcggca tggttgattt gccgctgctg
    23161 tcggtcccct cgctacaaca acaaatggtg gggcatcgct cggcaaacat gacgctggaa
    23221 cagcttgaat ctctcaatgc agagcagaaa gcgcgcacag tgttggtggt gcaggacccc
    23281 tttaccagct attacgatgc gcaagtggtg gcggattttg tccgtctggt cgaaaaatta
    23341 ggtttccagc ctgtgttact gccattttcg ccaaatggca aagcccagca tattaaaggt
    23401 tttcttaatc gttttgcgaa gacggcgaaa aagacggcgg atttcctcaa ccgtatggcg
    23461 aagctgggta tgccaatggt gggcgtcgat ccggcgctgg tactttgtta tcgcgatgaa
    23521 tataaactgg cgctgggcga ggagcgtggc gagtttaacg tcttactggc gaatgaatgg
    23581 ctggcaagcg cacttgagtc acagccagtg gctacagtca gcggtgaatc atggtatttc
    23641 tttggtcact gtaccgaagt taccgccttg ccgggtgcgc cagcacaatg ggccgcgata
    23701 tttgcccgtt ttggcgcgaa actggaaaat gtcagcgtgg gttgctgcgg catggcaggg
    23761 acttacggac atgaagcgaa aaaccataaa aattcgctcg ggatctatga gttatcctgg
    23821 catcaggcta tgcagcgact gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt
    23881 agtcaggtca aacgggttga aggcacgggg gtacgccatc ctgtgcaggc tttactggag
    23941 attattaaat gatatggaaa cgaaaaatcc ccctggaagc actgaatgcc atgggtgaag
    24001 gaaacatggt ggggttactg gatattcgct ttgaacatat tggtgatgac acccttgaag
    24061 cgacaatgcc agtagactcg cggacaaagc agcctttcgg gttgctacat ggaggtgcat
    24121 ccgtggtact ggccgaaagt atcggttccg ttgccggtta tttatgtacc gaaggtgagc
    24181 aaaaagtggt tggtctggaa atcaatgcta accacgtccg ctcggcacga gaagggcggg
    24241 tgcgcggcgt atgcaaaccg ttgcatctcg gttcgcgtca ccaggtctgg cagattgaaa
    24301 tctccgatga gaaagggcgt ttgtgctgtt cgtcacgatt gacgaccgcc attttgtgac
    24361 gttgtcacca gaaaagtgtg acgcatattg cttttggtca ttatctgcac agtaaacggt
    24421 ggtatactgt gcaggtcttg tctaaaagcg aggatgctat gtctactcaa cttgatccca
    24481 cccagctggc cattgaattt ttacgccgtg accagagcaa tctttcacct gcccaatatc
    24541 ttaaacgtct gaaacaacta gaacttgaat ttgctgacct gcttactctc tcttctgctg
    24601 aattaaaaga agaaatctac tttgcctggc gtctgggcgt gcattgatcc tgacttaatg
    24661 ccgcaagttc gccaaatcga cctggattaa cctccgtcca ggttcgatga ccttcttaat
    24721 actgcttatc tacccgcctg atattgttca aaaataatac taatcgcagt ttgtgttaaa
    24781 acggcggctt agctttatga gattattctt atcgtccctt caagagctaa gccactgaga
    24841 gtgccggaga taagcgccgg atggggtaga aacccttaag cctgtgtcgc acagacttaa
    24901 gggttttctt atttctgaac gctgcacttt tttacctatc tcttaaatag tcattttcaa
    24961 tacaactttt tctttttcat cacagcctta atgctcctta gataataatc attatctaac
    25021 aatgagatac ctaattctta gcgtgcctgt taacccacac atcagggtct atgcttatta
    25081 aaagcaggca aaaagcatgt tttacggtaa agcccctgcc tttgctgggt tgaactgata
    25141 atcattatca ctaacatgct gttatacgct gaaagcgatg aagtgaggta aatcgatgga
    25201 catgcattca ggaaccttta atccacagga tttcgcctgg caaggcttaa cgctgacacc
    25261 cgcagccgct gtccacatcc gcgagctggt ggcaaagcag ccgggtatgg tcggcgtgcg
    25321 cttaggtgtg aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca gtgttagcga
    25381 gccggacaaa gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg tcccgctgca
    25441 agcgatgccg tttattgatg gcacggaagt cgatttcgtt cgtgaaggac ttaatcagat
    25501 attcaaattt cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa gctttggggt
    25561 ataggcggta ctatgtctcg taatactgaa gcaactgacg atgtcaaaac ctggaccggc
    25621 ggcccgctga attataaaga aggattcttc acccagttag ccaccgatga gctggcaaag
    25681 gggataaacg aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc ggagtggatg
    25741 ctggagtttc gtctaaacgc ctatcgcgca tggctggaga tggaagaacc gcactggttg
    25801 aaagcgcact acgacaagct gaattatcag gattacagct actactcggc accatcgtgc
    25861 ggtaattgtg acgacacttg cgcgtctgaa cctggcgcgg tgcagcaaac tggcgcgaac
    25921 gcctttttaa gtaaagaggt ggaggcggcg tttgagcagt tgggtgttcc cgtgcgggaa
    25981 ggcaaagagg tggcggtgga tgccattttc gactcagttt cggttgccac tacttatcgc
    26041 gaaaaactgg cggagcaggg aattattttc tgttcctttg gtgaggcgat ccacgatcac
    26101 ccggaactgg tgcgtaaata tctcggcacc gtggtgccgg ggaatgacaa cttctttgcc
    26161 gcgcttaatg cggcggtagt ctctgacggt acgtttattt atgtgcctaa aggcgtgcgc
    26221 tgcccgatgg aactttccac ctattttcgc attaacgcgg aaaaaaccgg acagtttgag
    26281 cgcaccattc tggtggccga cgaagacagc tacgtcagct acattgaagg ctgttccgct
    26341 ccggtgcgtg acagctatca gttgcacgcg gcagtggtgg aagtcatcat ccataaaaac
    26401 gccgaggtga aatattccac ggtacaaaac tggttccctg gcgataacaa caccggcggt
    26461 attctcaact tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa aatgtcatgg
    26521 acgcaatcag aaaccgggtc agcgattacg tggaaatatc ccagctgcat tttgcgcggc
    26581 gataactcca ttggtgagtt ttactcagtg gcgctgacca gcggtcatca gcaagcggat
    26641 accggcacca agatgatcca catcggtaaa aacaccaaat cgaccattat ctcgaaaggg
    26701 atctctgccg gacatagcca gaacagttat cgcggcttag tgaaaatcat gccgacggca
    26761 accaatgcgc gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa ttgtggggcg
    26821 cataccttcc cgtatgtcga atgtcgcaat aacagcgcac aactggagca cgaggcaacg
    26881 acatcacgta ttggtgaaga tcaactgttt tactgcctgc aacgcgggat cagcgaagaa
    26941 gacgccatct cgatgattgt taacggtttc tgcaaagacg tgttctcaga actgccgctg
    27001 gaatttgccg ttgaagcaca aaaactcctc gccatcagtc ttgaacacag cgtcggataa
    27061 ggaataaaca tgttaagtat taaagattta cacgtcagcg tggaagataa agctatcctg
    27121 cgtggattaa gcctcgacgt tcgtcccggc gaagttcacg ccattatggg gccaaacggt
    27181 tcgggcaaaa gtaccttatc ggcaacgctt gccgggcgag aagattatga agtgacgggc
    27241 ggcacggttg agttcaaagg caaagatttg cttgcactgt cgccggaaga tcgtgcaggc
    27301 gaaggcatct ttatggcctt ccagtatccg gtggagattc caggtgtcag taaccagttt
    27361 ttcctgcaaa cggcacttaa tgcggtgcgc agctatcgcg gccaggaaac gctcgaccgc
    27421 tttgattttc aggatttgat ggaagagaaa atcgctctcc tgaagatgcc ggaagattta
    27481 ttaacccgtt cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa cgatattttg
    27541 caaatggcgg tgctggaacc ggagttatgc attcttgatg aatcggactc cgggctggat
    27601 atcgacgcat taaaagtggt cgccgatggc gtgaactcgc tgcgtgatgg caagcgctca
    27661 ttcatcattg ttacgcacta ccaacgcatt ctcgactaca tcaagcctga ttacgttcat
    27721 gtgctatatc agggacgaat tgtgaaatcc ggcgatttca cgttggtcaa acaactggag
    27781 gagcagggtt atggctggct taccgaacag cagtaacgcg ctgcaacagt ggcatcactt
    27841 gtttgaagct gaagggacaa aacgctcccc gcaagcacag cagcatttac aacaattgct
    27901 gcgtaccgga ctgccgacac gtaaacatga aaactggaaa tatacgccgc tggaagggct
    27961 gaccaatagt cagtttgtca gcattgcggg agagatatcc ccacagcagc gagatgcctt
    28021 agcgttaacg ttagacgctg tgcggctggt atttgtcgat ggacgttacg tgcctgcgct
    28081 gagcgatgcg actgaaggca gcgggtatga agtgagcatt aacgacgacc gtcaggggtt
    28141 acccgatgct attcaggcgg aagtgtttct gcatttgacg gaaagcctgg cacaaagcgt
    28201 gacgcatatc gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt taatgcatat
    28261 cacccagggc gtggcaggtg aagaggtgaa cactgcccat taccgacatc atctggagct
    28321 ggcggaaggt gccgaagcaa cggtgatcga acattttgtc agccttaatg atgctcgtca
    28381 ttttaccggg gcacggttct ctatcaacgt cgcagcgaat gcccacttgc agcatatcaa
    28441 gctggcgttt gaaaacccgc tcagtcacca ctttgcccat aacgatttgt tgctggctga
    28501 cgatgccacc gcatttagcc acagtttcct gctgggtggc gcagtgttac gacacaacac
    28561 cagtacgcaa ctcaatggcg aaaactgcac gctgcggatc aatagcctgg cgatgccggt
    28621 gaaaaacgag gtgtgtgata cccgtacctg gctggaacac aataaaggtt tttgtaacag
    28681 ccgacagttg cacaaaacta tcgtcagcga caaaggccgc gcggtattta acggtttgat
    28741 caacgtcgcg cagcacgcca tcaaaacgga tggtcagatg accaacaata atctgctgat
    28801 gggcaaactg gcggaagtgg atacgaaacc gcagctggaa atctatgcag atgatgtgaa
    28861 atgcagccac ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct atctgcgctc
    28921 gcgcgggatc aatcagcagg atgcccagca gatgatcatt tacgcctttg ctgccgaact
    28981 gacggaagca ctgcgtgatg aggggcttaa acagcaggtg ctgacccgaa tcggtcaacg
    29041 gctgccagga ggtgcaagat gactttttcc gtcgacaaag tgcgggccga ctttccggtg
    29101 ctttcgcgtg aggtaaacgg tttgccgctg gcttatctcg acagcgccgc cagtgcgcag
    29161 aaaccgagcc aggtgattga cgccgaggcc gagttttatc gtcatggcta cgcggcggtg
    29221 catcgcggta ttcatacctt aagcgcccag gcgaccgaga aaatggagaa cgtgcgcaag
    29281 cgggcatcgc tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt ccgcggcacg
    29341 acggaaggga tcaatctggt cgccaatagc tggggcaaca gcaacgtgcg ggcgggcgat
    29401 aacatcatca taagtcagat ggagcaccac gctaacattg ttccctggca gatgctttgc
    29461 gcacgcgttg gcgcagagct gcgtgtgatc ccgctcaatc ccgacggtac gttgcaactg
    29521 gagacgctgc ctacgctgtt tgatgagaaa actcgcctgc tggcaattac tcatgtctcc
    29581 aacgtgcttg gcacagaaaa tccactggcg gaaatgatca cgcttgcgca ccagcatggc
    29641 gcaaaagtgc tggtggatgg cgctcaggcg gtgatgcatc atccggtgga tgttcaggcg
    29701 ctggattgcg atttttacgt gttttccggg cataaactgt atggccccac cgggattggc
    29761 attctttatg tcaaagaagc cttgctacag gagatgccgc cgtgggaagg gggcggttct
    29821 atgatcgcca ccgtcagcct gagtgaaggc actacctgga ccaaagcacc gtggcggttt
    29881 gaagccggta cacccaatac cgggggcatc attggtcttg gcgcggcgct ggagtatgtt
    29941 tcggcgcagg ggcttaataa catagccgag tatgaacaga atctgatgca ttacgcgcta
    30001 tcacagctgg aatctgtacc ggatctcact ctctatggcc cacaaaacag gcttggcgtt
    30061 attgctttta atctcggtaa acaccacgcc tatgatgttg gcagttttct cgataattac
    30121 ggcattgctg tgcgtaccgg acatcactgc gctatgccat taatggccta ttataacgtc
    30181 cctgctatgt gtcgggcgtc gctggccatg tataacaccc atgaagaagt ggatcgtctg
    30241 gtgaccggac tgcaacgtat tcaccgtctg ctgggataac agggaggcac tatggcttta
    30301 ttgccggata aagaaaagtt gctgcgtaat tttttacgct gcgccaactg ggaagagaaa
    30361 tatctctaca ttattgagct gggccagcgt ctgccagaat tacgcgacga agacagaagc
    30421 ccacaaaata gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg tcagaatgcg
    30481 cagggaatta ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg gcttattgcg
    30541 gtcgtcttta ttctctacga tcagatgacg ccgcaggatg ttgtcaattt cgatgtgcgt
    30601 ccgtggtttg aaaaaatggc actcacccaa catctcaccc catctcgttc acaaggtctg
    30661 gaagcgatga ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac taaagggaca
    30721 gctttcatcc ggttggctca cattgccgga tggtcaatgt ttacactgac ctgcacagga
    30781 gtgtttcggt ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag tctccggcaa
    30841 atacaggagg tttacaatga aacgcgcgtc tttgcttaca ctcacgctta tcggcgcttt
    30901 tagtgccatc caggctgcct gggcggttga ttatccgcta ccaccaaccg gaagccgact
    30961 ggttgggcaa aatcaaacgt atacggtgca ggaaggggat aaaaaccttc aggccatcgc
    31021 ccgacgtttt gatactgcgg caatgttgat ccttgaagcc aataacacta tcgccccggt
    31081 gccaaaaccc ggtacgacga taactattcc ttcgcaactg ttattacctg atgcgccgcg
    31141 tcaggggatt atcgttaacc ttgcagagct gcgcctttat tattatccgc cgggagaaaa
    31201 tatcgtgcag gtttatccga taggtattgg cttgcagggg ctggaaacgc cagtgatgga
    31261 aacgcgtgtg gggcagaaaa tccctaaccc aacctggacg cctacggcag gcattcgtca
    31321 acgttcgctg gagcgtggca ttaaattacc gccagtcgtt cctgccggac caaataaccc
    31381 gctaggacgt tacgcactgc gcctcgcgca tggtaatggc gaatacctca ttcatggcac
    31441 cagtgcgccg gacagcgtcg gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc
    31501 ggatattaaa gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga
    31561 accagtgaaa tattccgtgg agcctaacgg gatgcgttat gttgaagtac atcgaccact
    31621 atcggcagaa gaacagcaga acgttcagac aatgccatac acactgccag caggctttac
    31681 gcaatttaaa gacaataagg ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg
    31741 ggcagggtat ccggttgcgg tgagcagtgg agcaactccc acagccagca atgcgccttc
    31801 agtagagtca gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg cgacgcagta
    31861 gtggtaaata gcagacaaaa aaatggcgca caatgtgcgc catttttcac ttcacaggta
    31921 ctattacttg cggtatttag tagccatgtt gtccagacgc tggttagcac gagctgcgtc
    31981 atctttagca gcctgaacgt cggaacgcat tgcgttcacg tcgttgctca gctggtcaac
    32041 tttagcgttc agagtctgaa cgtcagaaga cagctgatcg attttagcgt tgctggagca
    32101 acctgccagc agagtagaac ccaggattac cgcgcccagt accagtttag tagctttcat
    32161 tattaatacc ctctagattg agttaatctc catgtagcgt tacaagtatt acacaaagtt
    32221 ttttatgttg agaatatttt tttgatggga aggcacttat ttttgatcgt tcgctcaaag
    32281 aagcatcgaa cgctgggttt tagata
//
LOCUS       DAZFTN010000053        32209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFTN010000053 DAZFTN010000000
VERSION     DAZFTN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..310
                     /locus_tag="ACHKV2_002619"
     CDS             <1..310
                     /locus_tag="ACHKV2_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940483.1"
                     /translation="GFYMRNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILS
                     LTTTLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR
                     "
     gene            complement(357..1244)
                     /gene="rarD"
                     /locus_tag="ACHKV2_002620"
     CDS             complement(357..1244)
                     /gene="rarD"
                     /locus_tag="ACHKV2_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO6940484.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1296..1763)
                     /gene="yigI"
                     /locus_tag="ACHKV2_002621"
     CDS             complement(1296..1763)
                     /gene="yigI"
                     /locus_tag="ACHKV2_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO6940485.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1928..2797
                     /gene="pldA"
                     /locus_tag="ACHKV2_002622"
     CDS             1928..2797
                     /gene="pldA"
                     /locus_tag="ACHKV2_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO6940486.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2930..4759
                     /gene="recQ"
                     /locus_tag="ACHKV2_002623"
     CDS             2930..4759
                     /gene="recQ"
                     /locus_tag="ACHKV2_002623"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO6940487.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4823..5443
                     /gene="rhtC"
                     /locus_tag="ACHKV2_002624"
     CDS             4823..5443
                     /gene="rhtC"
                     /locus_tag="ACHKV2_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO6940488.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5505..6125)
                     /gene="rhtB"
                     /locus_tag="ACHKV2_002625"
     CDS             complement(5505..6125)
                     /gene="rhtB"
                     /locus_tag="ACHKV2_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO6940489.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6236..7258
                     /gene="pldB"
                     /locus_tag="ACHKV2_002626"
     CDS             6236..7258
                     /gene="pldB"
                     /locus_tag="ACHKV2_002626"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO6940490.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7266..8066
                     /gene="yigL"
                     /locus_tag="ACHKV2_002627"
     CDS             7266..8066
                     /gene="yigL"
                     /locus_tag="ACHKV2_002627"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO6940491.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKEPLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8142..9041
                     /gene="bioP"
                     /locus_tag="ACHKV2_002628"
     CDS             8142..9041
                     /gene="bioP"
                     /locus_tag="ACHKV2_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO6940492.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8929..9882)
                     /gene="metR"
                     /locus_tag="ACHKV2_002629"
     CDS             complement(8929..9882)
                     /gene="metR"
                     /locus_tag="ACHKV2_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO6940493.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10118..12379
                     /gene="metE"
                     /locus_tag="ACHKV2_002630"
     CDS             10118..12379
                     /gene="metE"
                     /locus_tag="ACHKV2_002630"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO6940494.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12419..13234)
                     /gene="ysgA"
                     /locus_tag="ACHKV2_002631"
     CDS             complement(12419..13234)
                     /gene="ysgA"
                     /locus_tag="ACHKV2_002631"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO6940495.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13496..14257
                     /gene="udp"
                     /locus_tag="ACHKV2_002632"
     CDS             13496..14257
                     /gene="udp"
                     /locus_tag="ACHKV2_002632"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO6940496.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14398..15825
                     /gene="rmuC"
                     /locus_tag="ACHKV2_002633"
     CDS             14398..15825
                     /gene="rmuC"
                     /locus_tag="ACHKV2_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO6940497.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15920..16675
                     /gene="ubiE"
                     /locus_tag="ACHKV2_002634"
     CDS             15920..16675
                     /gene="ubiE"
                     /locus_tag="ACHKV2_002634"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO6940498.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16689..17294
                     /gene="ubiJ"
                     /locus_tag="ACHKV2_002635"
     CDS             16689..17294
                     /gene="ubiJ"
                     /locus_tag="ACHKV2_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO6940499.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17291..18931
                     /gene="ubiB"
                     /locus_tag="ACHKV2_002636"
     CDS             17291..18931
                     /gene="ubiB"
                     /locus_tag="ACHKV2_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO6940500.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19010..19279
                     /gene="tatA"
                     /locus_tag="ACHKV2_002637"
     CDS             19010..19279
                     /gene="tatA"
                     /locus_tag="ACHKV2_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO6940501.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19283..19798
                     /gene="tatB"
                     /locus_tag="ACHKV2_002638"
     CDS             19283..19798
                     /gene="tatB"
                     /locus_tag="ACHKV2_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO6940502.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19801..20577
                     /gene="tatC"
                     /locus_tag="ACHKV2_002639"
     CDS             19801..20577
                     /gene="tatC"
                     /locus_tag="ACHKV2_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO6940503.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20619..21401
                     /gene="tatD"
                     /locus_tag="ACHKV2_002640"
     CDS             20619..21401
                     /gene="tatD"
                     /locus_tag="ACHKV2_002640"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO6940504.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21398..21886)
                     /gene="rfaH"
                     /locus_tag="ACHKV2_002641"
     CDS             complement(21398..21886)
                     /gene="rfaH"
                     /locus_tag="ACHKV2_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO6940505.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22053..23546
                     /gene="ubiD"
                     /locus_tag="ACHKV2_002642"
     CDS             22053..23546
                     /gene="ubiD"
                     /locus_tag="ACHKV2_002642"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO6940506.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23592..24293
                     /gene="fre"
                     /locus_tag="ACHKV2_002643"
     CDS             23592..24293
                     /gene="fre"
                     /locus_tag="ACHKV2_002643"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO6940507.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24485..25648)
                     /gene="fadA"
                     /locus_tag="ACHKV2_002644"
     CDS             complement(24485..25648)
                     /gene="fadA"
                     /locus_tag="ACHKV2_002644"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO6940508.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25658..27847)
                     /gene="fadB"
                     /locus_tag="ACHKV2_002645"
     CDS             complement(25658..27847)
                     /gene="fadB"
                     /locus_tag="ACHKV2_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO6940509.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28037..29368
                     /gene="pepQ"
                     /locus_tag="ACHKV2_002646"
     CDS             28037..29368
                     /gene="pepQ"
                     /locus_tag="ACHKV2_002646"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO6940510.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29368..29982
                     /gene="yigZ"
                     /locus_tag="ACHKV2_002647"
     CDS             29368..29982
                     /gene="yigZ"
                     /locus_tag="ACHKV2_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO6940511.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30021..31472
                     /gene="trkH"
                     /locus_tag="ACHKV2_002648"
     CDS             30021..31472
                     /gene="trkH"
                     /locus_tag="ACHKV2_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO6940512.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31484..32029
                     /gene="hemG"
                     /locus_tag="ACHKV2_002649"
     CDS             31484..32029
                     /gene="hemG"
                     /locus_tag="ACHKV2_002649"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO6940513.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7495 a         8151 c         8795 g         7768 t
ORIGIN      
        1 tggattctat atgagaaatg gtaacttttg gcagttgttc ttgggtaaag aaccttacag
       61 caaaaaatct gattttgtta aggcatggtt attgtggtgg gctattacca tccttgttct
      121 atttatttta agcctgacta cgacattaaa tttaagaagt ataggtatag ggttaagttt
      181 tgggttttgt caaatccttg gtagatatac gatgtctcta ggatggagta agtactgggg
      241 agttttgggg ggatttcctg taacaaatat tgttcttttt cttatcttaa tttttttcaa
      301 aaaaagataa ataagtcagt gggtaggctc tttaaacggc ctacccatgt taattcttaa
      361 ctttttctac gctgcgtata aatcgcatcc atcacaaaaa ttgccagcgc cacccaaata
      421 aaggtgaaag tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc
      481 aggaacatca gcgtcgggcc aatgtactgg aaaaagccta acgttgagag acgaaggcgc
      541 gtggcagcgg cggtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc
      601 agtaaattca gcgacatcgg gttttgcccc atatggctgg ttgagctgtc ggcaatagca
      661 aacaggtaaa tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt
      721 gcttctaccg caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc
      781 agcgcgataa taggtagcga accaaaagtc cacagctgga ctaacacgcc acatatcgcc
      841 agaatcaccg ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc
      901 acaatgttca ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg
      961 ttcaccgccc agataaacag tagccagttg ccaccaatca gcacggcaga gactgccagc
     1021 ataaaaattt tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg
     1081 ctcatcagca ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg
     1141 gcgggcacgt agtaaatcaa cttgaagtac gctggggcta taccccaaat aaaataagcg
     1201 gcaagagcga gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt
     1261 taattggtaa cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg
     1321 gcactggcaa tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta
     1381 ttgcctgcac gcaacaggct actggtagca gtaaaacgct cgcctctgcc tgggcgcaga
     1441 taatcgacgc gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca
     1501 ctgatggttt cgtggcgggt taaggtactt cctacgcaca ccagaccggc ggcgacatcc
     1561 agcgccgacg caatgacccc gccgtgcaaa atgctttgcg cccagttgcc caccatcatt
     1621 ggctgatttt taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc
     1681 aatgcgcggt taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt
     1741 tcagcggtca gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat
     1801 tttatgattc tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt
     1861 aagtatgtaa aataccccgc agaaaaacat tcaccttatc aataattcgt tacggagaac
     1921 acgaccgatg cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt
     1981 atatgcacaa gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat
     2041 tatcgccaat atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa
     2101 ctacctcatc tacacccaaa ccagcgatct gaacaaagaa gcgattgcca gttacgactg
     2161 ggcggaaaat gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg
     2221 gcgtgggatt ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg
     2281 gcaactgtcc aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt
     2341 gttcctcggt tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat
     2401 ggggtataac cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct
     2461 ttatactcgc ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt
     2521 ggtgggtaat actgacgata acccggatat caccaaatat atgggttact accagcttaa
     2581 aatcggctat cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac
     2641 cggctacggc ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta
     2701 tactcaggtt tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg
     2761 tgtcggtgtg ggggttatgc taaacgattt gttttgatga acggttgagt ggttggcaaa
     2821 tctggaatcc agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg
     2881 caggcgctga aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc
     2941 ggaagtgttg aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca
     3001 acagtttcgc cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct
     3061 ggtcgtcatg cccaccggtg gcggaaaatc cctttgctat caaattcctg ccttattgct
     3121 aaacggcctt accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca
     3181 actgcaagcc aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca
     3241 acttgaagtg atgacaggct gccgcaccgg gcaaattcgt ctgctttata tcgccccgga
     3301 acgcctgatg ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc
     3361 cgttgatgaa gcgcactgta tctcccaatg gggccacgat ttccgcccgg aatatgccgc
     3421 gctcggtcag ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc
     3481 cgacgacacc acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca
     3541 aatcagcagt tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct
     3601 cgatcagttg atgcgctacg tgcaggaaca gcgcggtaag tccggcatta tctactgcaa
     3661 cagccgggcg aaagtagaag acaccgctgc gcgcctgcaa agcaagggta ttagcgcggc
     3721 ggcctatcat gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg
     3781 cgatgacctg caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa
     3841 cgttcgcttc gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac
     3901 cggtcgcgcc gggcgtgatg gcctgcctgc ggaagcgatg ttgttttacg atccagctga
     3961 tatggcgtgg ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga
     4021 gcgccacaaa ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt
     4081 attgctgaac tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct
     4141 cgatccgccg aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg
     4201 tcgtgtgaat cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa
     4261 ccagcgtatc cgcgactatg gtcatgacaa actgaaagtc tatggcatgg gccgtgataa
     4321 aagccatgaa cattgggtga gcgtgatccg ccagctgatt cacctcggcc tggtgacgca
     4381 aaatattgcc cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg
     4441 cgaatcctct ttacaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca
     4501 gaaatcgttc ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa
     4561 atcgattgcc gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat
     4621 tgagatggct gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg
     4681 gatgcgcaag ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga
     4741 tggcgacgac gaagagtagt cagcagcata aaaaagtgcc aggatgaaga ctccgtaaac
     4801 gtttcccccg cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat
     4861 tgtggcgctt atgagtcccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg
     4921 ttcccgtaaa gaagcgatga tgggcgtgct gggtattacc tgcggcgtta tggtttgggc
     4981 agggattgcg ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct
     5041 gattatggtt ggcggtggcc tgtatctctg ctggatgggt taccagatgc tgcgcggcgc
     5101 gctgaaaaaa gaggcggttt ctgcacctgc gccacaggtc gagctggcga aaagtgggcg
     5161 cagtttcctg aaaggtttac tgaccaatct cgctaatccg aaagcgatta tctactttgg
     5221 ctcggtgttc tcattgtttg tcggtgataa cgttggcact accgcgcgct ggggcatttt
     5281 cgcgttgatt attgtcgaaa cgctggcgtg gtttactgtg gtggccagcc tgtttgccct
     5341 gccgcaaatg cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc
     5401 gttatttgcc ggatttggca ttcatttgat tatttcgcgg taatgccaga cgcgtcttca
     5461 gagtaagtcg gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc
     5521 gatgctaaca gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc
     5581 atctgctttg gtcctttaat ccatagagca atccgttgag caagggtggc gtaaccgatc
     5641 atcacaataa tatcgaccac aatagtggtg acaccgagca cgatatactg catcagttgc
     5701 ggctgttgcg gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga
     5761 ttggtgagat tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc
     5821 agcgatttaa gatcaattgc gccagcggcg cgccattgct gtattcccag ccaaatcaag
     5881 taagccgcgc ctgcccactt caacacttca aacgcaatca ctgagcggga aaatagcgtc
     5941 cccaacccca cgccaaccag cacaatatga atcgccagtc cggtctgaag cccggcaata
     6001 gacgccaccg cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca
     6061 gagcctggcg acaggcttaa aatgattgat gtcagtaggt aggcaaacca ccattctaag
     6121 gtcatgatga actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt
     6181 gtgtcaggca cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt
     6241 tcagcagcaa aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc
     6301 gctgactgat ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc
     6361 ggtgcgcttt gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg
     6421 gcgtattgag agttacgtaa aatatgcgga actggcctac gatttgttcc atttggggtt
     6481 tgatgtctta atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc
     6541 gcatctcggg catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca
     6601 acaggaggtc cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg
     6661 tgcgatctcc acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac
     6721 tgcgccaatg tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa
     6781 ctgggccgaa gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg
     6841 cgcgttgccg tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt
     6901 acgcttctat gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg
     6961 cgaaagtatt ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac
     7021 gcttctcttg caggctgaag aggaacgcgt ggtggataac cgcattcatg accgtttttg
     7081 tgaactccgc accgccgcgg gccatcctgt cgaaggggga cgaccgctgg taattaaagg
     7141 tgcgtaccat gagatccttt ttgaaaagga cgcaatgcgc tcagttgcgc tccacgccat
     7201 cgttgatttt ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat
     7261 ttcttatgta ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata
     7321 cattatcccc ttacgccaaa gaacccctga agctgctcac cgcgcgcggc atcaactttg
     7381 tgtttgcgac cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta
     7441 agtcttacat gattacctcc aatggtgcgc gcgttcacga tctggatggt aatctgattt
     7501 ttgctcataa cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc
     7561 cggacatcat tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg
     7621 aagagatgcg cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag cctggattac
     7681 tggagccgga aggcgtcagc aaagtgttct tcacctgcga ttcccatgaa caactgctgc
     7741 cgctggagca ggcgattaac gctcgttggg gcgatcgcgt caacgtcagt ttctctacct
     7801 taacctgtct ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg
     7861 cgaagaaact gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgatg
     7921 ccgaaatgct gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga
     7981 aagaccttca tcccgagctg gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt
     8041 atctgcgtaa actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac
     8101 ttcgctacaa tggatactcg ttaatcaaag agttttccat tgtggcgcta cttatcatca
     8161 ccacgattct gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg
     8221 atagctattt tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc
     8281 tgcgtacccg tggcaatagc ctgaaaacag tcggcctgta tatgctggtg ggcgcgatgc
     8341 agctcggcgt gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtttccgaac
     8401 tgctgctgtt caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta
     8461 agcgccgtct gcgctgggga tatgctttta gtgccttgtt ggcggtgatt ggtgccggga
     8521 ttattcgcta tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct
     8581 ccaatatcac ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga
     8641 tgccgcagca taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg
     8701 catggttctt gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc
     8761 tggtgtttct tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcaa
     8821 cccaagtaga cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc
     8881 tggttaacct ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc
     8941 tggtgatcct ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa
     9001 cggcagatga tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg
     9061 acgctgctcg ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt
     9121 tttggtcacc accagaccct ggcgctcaaa actctctact acccaatgtg gtagcgcggc
     9181 aatacccatc cgcgcggcaa ccatctgaat caacaataag gtgttatcga cgcttttcag
     9241 cgacgggcta acgcctgcgg gctggagaaa atgccgccag acatccagtc ggctacgctg
     9301 caccggataa atcaacagcg tttcgctggc gagatcttcc ggcgtgattc gcgttttcgc
     9361 cgccagtgga tggtcaggtg ctaacaccag acgcacttca tagtcgaaca tcggcgaata
     9421 atgcaggcca ctacgcggca gaatatcgga ggtcatcacc agatccagct cgccctgctg
     9481 caacgcaggc tgcgggtcaa acgttacgcc cgatttaaaa tccatctcca cctgcggcca
     9541 gttcttatgg aaattttcta atgcgggcgt cagccactga atacagctat ggcactcaat
     9601 ggcaatgcgc aggcgcgtct gttgcggttc attgcaggct tgcagcgcct ggctaatttg
     9661 cggcagcacc tggtttgcca gttgcaacag gatttccccc tgtggagtaa agcgtagcgg
     9721 ctggctctta cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg
     9781 agacagggcg gattgcgttt gatgcaacgt cgccgcagcg gctgcgagag agccgcagtt
     9841 ccgcaacgct tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg
     9901 catgaataat ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc
     9961 tggacggcta aaatccttcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc
    10021 cggtcactta cttcagtaag ctcccgggga tgaataaact tgccgccttc cctaaattca
    10081 aaatccatag gatttacata taattagagg aagaaaaatg acaatattga atcacaccct
    10141 cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaga gttattgggc
    10201 ggggaactcc acgcgtgaag aactgctggc ggtagggcgt gaattgcgtg ctcgtcactg
    10261 ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga
    10321 tcatgtactg accaccagtc tgctgctggg taacgttccg gcgcgtcatc agaacaaaga
    10381 tggttcggta gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga
    10441 acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc
    10501 ggagttcgtt aaaggccaac agttcaaact gacctggacg cagctgctgg acgaagtgga
    10561 cgaggcgctg gcgctgggcc acaaggtgaa acctgtgctg ctggggccga ttacctggct
    10621 gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctg agcctgctga acgacattct
    10681 gccggtttat cagcaagtgc tggcagaact ggcgaaacgt ggcatcgagt gggtacagat
    10741 tgatgaaccc gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc
    10801 ttacgacgcg ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac
    10861 gccaaatctc gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca
    10921 tggtaaagat gacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc
    10981 gggtctgatc aatggtcgta acgtctggcg cgccgatctt actgagaaat atgtgcaaat
    11041 taaggacatt gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag
    11101 ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt
    11161 cgccctacaa aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac
    11221 ggcagctctg gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca
    11281 taatccggcg gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa
    11341 tgtctatgaa gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac
    11401 caccacgatt ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa
    11461 aaagggcaat ctcgacgcca ataactaccg cacgggcatt gcggaacata tcaggcaggc
    11521 cattgttgaa caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa
    11581 tgacatggtg gaatactttg gcgagcacct cgacgggttt gtctttacgc aaaacggttg
    11641 ggtacagagc tacggttccc gctgcgtgaa gccaccgatt gttattggtg acgttagtcg
    11701 cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa tctatgactg ataaaccggt
    11761 gaaagggatg ctgactggcc cggtgaccat tctctgctgg tcgttcccgc gtgaagatgt
    11821 cagccgtgaa accatcgcca aacaaattgc gctggcgctg cgtgatgaag tggcggacct
    11881 ggaagccgca ggaattggca tcatccagat tgatgaaccg gcgctgcgcg aaggtttacc
    11941 gctgcgtcgt agcgactggg atgcatatct ccagtggggc gtggaggcct tccgtatcaa
    12001 cgccgccgtg gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa
    12061 cgacatcatg gattcgattg cggcactgga cgcagacgtc atcaccatcg aaacctcgcg
    12121 ttctgacatg gagttgctgg agtcgttcga agagtttgat tatccgaatg aaatcggtcc
    12181 aggcgtctat gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct
    12241 gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct
    12301 gaaaacgcgc ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca
    12361 gaacttgcgt cgggggtaaa atccaaaccg ggtggtaata ccacccggtc ttttctcatt
    12421 acagcgactt cttcccacca tactgcttaa accattccag catacgctgc cagccatctt
    12481 ctgcagatgc ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc ccggcgtccg
    12541 ggtacacgat aatctctgct ttcgcattag cagcccgcag cgcctggcgc atggtttcaa
    12601 cgctctcctg cggaatactg ttatccagac cgccatataa gccgagaacc ggcgcgttaa
    12661 gatcggttgc gatatcaaca ggttgtttcg gtgaattcag cgacttatcg cccgtcagtt
    12721 tgccgtacca cgccactgcg gcttttagct gtggattatg cgcggcatac agccaagtga
    12781 tacgtccacc ccagcagaat ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg
    12841 aagcccaact ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc acttttgcta
    12901 ccagaccgct aagcaacgtg gggatatcgg caaaatcatt cggatcgcct tcgcggaagt
    12961 aaagttcagg tgcgatagcc agatacccct ccagcgccag acggcgacaa atgtcgcgga
    13021 tatgttcatg cacgccaaaa atttcctgca ctacaatgac cactggcagt gggccatcgc
    13081 tttgctttgg tctggcatgg taagcaggca tgttatcccc ttgtgaaggg atagaggtga
    13141 agcccgccac aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga ggcgatgcag
    13201 caggtgcaaa tccagattgt tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat
    13261 gttgcgcaat gttaactata gtcagcatgc aacaaatcac attgcctgaa tcggctcatc
    13321 ttttatgcag tcctgcagaa tgaagagtga tttatgtgat ttgcatcacc tttggtgggt
    13381 aaatttatgc aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt
    13441 cggtaaagtg tctttttgct tcttctgact aaaccgattc acagaggagt tgtatatgtc
    13501 caagtctgat gtttttcatc tcggcctcac taaaaacgat ttacaagggg ctacgcttgc
    13561 catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt
    13621 taagctggca tctcaccgcg aattcactac ctggcgtgca gagctggatg gtaaacctgt
    13681 tatcgtctgc tctaccggta tcggcggccc gtctacctct attgctgttg aagagctggc
    13741 acagctgggc attcgcacct tcctgcgtat cggtacaacg ggcgctattc agccgcatat
    13801 taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg cgagcctgca
    13861 cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg cgctggttga
    13921 agctgcgaaa tccattggcg cgacaactca cgttggcgtg acagcttctt ctgatacctt
    13981 ctacccaggt caggaacgtt acgatactta ctctggtcgc gtagttcgtc actttaaagg
    14041 ttctatggaa gagtggcagg cgatgggcgt aatgaactat gaaatggaat ctgcaaccct
    14101 gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa
    14161 ccgcacccag caagagatcc cgaatgctga gacgatgaaa caaactgaaa gccaagcggt
    14221 gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc
    14281 gacgcgttcg gccttttgta tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg
    14341 acgtttgtac agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg
    14401 gatttctcaa tcatggttta cgcagttatt gcgttggtgg gtgtggcaat tggctggctg
    14461 tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg
    14521 gtcgcggagt taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgtgcagag
    14581 tgcgagttac tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc
    14641 gatctgcgtg aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt
    14701 cgccagatga ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt
    14761 atttttgagc acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg
    14821 ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt
    14881 aaagaagcac aagaacgcca taccctgacc cacgaaattc gcaatctcca gcaactcaac
    14941 gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc
    15001 cagggcaact ggggcgaggt agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa
    15061 gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg
    15121 gatgtcatcg tgcggctgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg
    15181 gtcgcctatg aacgctattt taacgccgaa gacgactaca cccgcgaaag cgcgctacag
    15241 gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag
    15301 ctgccggggc tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt
    15361 ttactggcgc ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caatatcatg
    15421 ctggttagcc cgactacgct gctggtggcg ctgcgcacta tcgccaacct gtggcgttat
    15481 gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag
    15541 atgcgtttgt tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat
    15601 aactatcggc aggcaatgaa aaaactctct tcagggcgcg gaaatgtgct ggcgcaggca
    15661 gaagcgtttc gcggtttagg agtagaaatt aaacgcgaga ttaatccgga tttggctgaa
    15721 caggcggtga gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat
    15781 gaagcttatc aacgcgatga tgaatataat cagcagtcgc gctagcccat tgggagtagt
    15841 taagccgggt agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt
    15901 ttgatgagca ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca
    15961 gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc
    16021 aaaatacgat gtcatgaatg atttgatgtc atttggtatt catcgtttgt ggaagcgatt
    16081 cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac
    16141 cggcgacctg acagcgaaat tctcccgcct ggtcggagaa actggcaaag tggtccttgc
    16201 tgatatcaat gaatccatgc tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat
    16261 tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt
    16321 tgattgcatc accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg
    16381 ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc
    16441 aattatcgag ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat
    16501 tggctcactg gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat
    16561 gcatcccgat caggataccc tgaaagccat gatgcaggat gccggattcg aaagtgtcga
    16621 ctactacaat ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga
    16681 gaccggaaat gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct
    16741 tcctgtatcg ctcacccgcg ctgaaaacgg cccgctcgcg tctgttgggc aaagtgctgc
    16801 gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg
    16861 atgtactggg cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt
    16921 tgccgaaact tcgcgatcgc cagcagcttg ccgccctgat tcgcagtggt gagctggaag
    16981 tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct ggcaggtctg gcagagttcg
    17041 accctgcgga actgctggcc ccttataccg gtgatatcgc cgctgaagga atcagcaaag
    17101 ccatgcgcgg aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg
    17161 cggaagccat tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg
    17221 cggaagagac ggctgccgtc gagcgtgctg ttgatgccct gatcaaacgg ctggaaaaac
    17281 tggaggctaa atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt
    17341 aagctacgga cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg
    17401 gcgatactca ttattctgga tgccaaatcg gcataaagac aaacttttag gtgagcgact
    17461 acgactggcc ctgcaagaac tggggccggt atggatcaag ttcgggcaaa tgttatcaac
    17521 ccgccgcgat ctttttccgc cgcatattgc cgatcagctg gcgttattgc aggacaaagt
    17581 tgctccgttt gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc
    17641 ggtagaagcg tggtttgacg attttgaaat caagccactg gcttctgctt ctatcgccca
    17701 ggttcatacc gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc
    17761 ggatattttg ccggtcatta aagcagacct gaaacttatc taccgtctgg ctcgctgggt
    17821 gccgcgtttg ctgccggatg gtcgccgtct acgcccaacc gaagtggtgc gcgagtacga
    17881 aaagaccttg attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg
    17941 gcgaaatttt gaagacagcc cgatgctcta catcccggag gtttaccctg actattgtag
    18001 tgaagggatg atggtgatgg agcgtattta cggcattccg gtgtctgatg ttgcggcgct
    18061 ggagaaaaac ggcaccaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac
    18121 tcaggtcttt cgcgacagct ttttccatgc cgatatgcac cctggcaaca tcttcgtaag
    18181 ctatgaacac ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct
    18241 aaacaaagaa gataaacgct atctggcaga aaactttatt gccttcttta atcgcgacta
    18301 tcgcaaagtg gcagagctac acgtcgattc tggctgggtg ccaccagata ccaacgttga
    18361 agagttcgaa tttgctattc gtacggtctg tgaacctatc tttgagaaac cgctggccga
    18421 aatttcgttt ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga
    18481 agtgcagccg caactggtgt tactccagaa aaccctgctc tacgtcgaag gggtaggacg
    18541 ccagctttat ccgcaactcg atttatggaa aacggcgaag cctttcctgg agtcgtggat
    18601 taaagatcag gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg
    18661 ggtcgaaaaa atgccagaac tgcctgaatt ggtttacgac agtttgcgcc agggcaagta
    18721 tttacagcac agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg
    18781 acaatcgcgt tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt
    18841 ggtcagccga cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc
    18901 ctggtttgtc ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta
    18961 acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat
    19021 cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa
    19081 gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag
    19141 cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat
    19201 cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca
    19261 cgataaagag caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtgtt
    19321 catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc
    19381 gggctggatt cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga
    19441 gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa
    19501 cctgacgccc gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa
    19561 gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa
    19621 cccggtggtg aaagataatg aagctgcgca tgagggtgta acgcctgccg ctgcacaaac
    19681 gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc
    19741 tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac
    19801 atgtctgtag aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg
    19861 ctgaactgca ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac
    19921 atctatcacc tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc
    19981 gccaccgacg tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg
    20041 attctgtcag cgccggtgat tctctatcag gtgtgggcat ttatcgcccc agcgctgtat
    20101 aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca gctctctgct attttatatc
    20161 ggcatggcgt tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc
    20221 gcgccggaag gggtgcaggt atccaccgac atcgccagct atttaagctt cgttatggcg
    20281 ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtgg caattgtgct gctgtgctgg
    20341 atggggatta cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca
    20401 ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct cgcaaacgct gttggcgatc
    20461 ccgatgtact gtctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg
    20521 cgaaatcggg aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt
    20581 caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt
    20641 tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg
    20701 gagttaatgg gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc
    20761 tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc
    20821 agtggcaagc tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg
    20881 cgattggtga atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac
    20941 gcgcttttgt tgcccagcta cgcattgccg cagatttaaa catgccggta tttatgcact
    21001 gtcgcgatgc ccacgagcgg tttatgacat tactggagcc gtggctggat aaactgcctg
    21061 gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcatg
    21121 gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcggactg gagctgcggg
    21181 aacttttgcc gttgattccg gcggaaaaat tactgatcga aactgatgcg ccgtatctgc
    21241 tccctcgcga tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc
    21301 atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca
    21361 cggatgctaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt
    21421 tcttcacact gtgcttaatc tctttattaa taagattaag caatagcatg gagcgagcct
    21481 caccatcggg ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg gtaataatca
    21541 ccttatcacc cggataaggg gttgccggat cgacaatgtc tttcggttta tataccgata
    21601 gctgatgaat caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc
    21661 tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca aattccacaa
    21721 acaggtagtt ggggaacaat ggctcactga ctgcagtacg tttgccacgc acgatttttt
    21781 ccagggtgat catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac
    21841 gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc
    21901 gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt
    21961 tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac
    22021 gccgtataat gggcgcagat taagaggcta caatggacgc catgaaatat aacgatttac
    22081 gcgacttctt gacgttgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg
    22141 acccgcatct ggaaatcact gaaattgctg accgcacgct gcgtgctggt gggcctgcgc
    22201 tgttgtttga aaaccctaaa gggtactcaa tgccggtgct gtgcaacttg ttcggtacgc
    22261 caaagcgcgt agcgatgggt atgggccagg aagatgtttc agcactgcgt gaagtcggta
    22321 aattattagc atttctgaaa gaaccagagc cgccaaaagg ttttcgcgat ctgtttgata
    22381 agctgccgca gtttaagcag gtgttaaaca tgccgacaaa gcgactgcgc ggtgcaccct
    22441 gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa ccgtattccc attatgacct
    22501 gttggccgga agatgccgcg ccgctgatta catgggggct aaccgttaca cgtggccctc
    22561 ataaagagcg acagaatctg ggcatttatc gccagcaact gattggtaaa aacaagctga
    22621 ttatgcgttg gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc
    22681 atccaggtga acgtttcccg atctctgtgg cgttgggcgc tgatccggca accattctcg
    22741 gtgcagtcac accagtacca gatactttgt cggaatacgc ctttgccgga ttgctacgtg
    22801 gcaccaaaac cgaagtagtg aagtgtattt ccaatgatct cgaagtgccc gccagtgcgg
    22861 agattgtgct ggaagggtat atcgaacaag gcgaaatggc gccagaagga ccgtatggtg
    22921 accacactgg ttactataac gaagtcgata gtttcccggt atttaccgtg acgcatatta
    22981 cccagcgtga agatgcgatt taccattcca cctataccgg gcgtccgcca gatgaacccg
    23041 cggtactggg agtggcgttg aatgaagtat ttgttcccat tctgcaaaag cagttcccgg
    23101 aaattgtcga tttttacctg ccgccggaag gctgctctta tcgcctggcg gtagtgacaa
    23161 tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac
    23221 gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgttaac gcacgcgact
    23281 ggaacgatgt gatttgggcg attaccaccc gtatggaccc agcgcgggat actgttctgg
    23341 tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa
    23401 aaatggggct ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc
    23461 ccatcaaaaa agatccagat gttgtcgcac atattgacgc catctgggat gaactggcta
    23521 tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac
    23581 agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata
    23641 ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt
    23701 tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcctcg acgccggatg
    23761 aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag
    23821 tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt
    23881 ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt
    23941 atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt
    24001 actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt
    24061 tgaagcatcc tggtctgcaa gtggtgccgg tggttgaaca accggaagcg ggctggcgtg
    24121 ggcgtactgg caccgtgtta acggcggtat tgcaggatca cggtacgctg gcagagcatg
    24181 atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg
    24241 agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa
    24301 aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttattca gtggcattta
    24361 gatctatgtc gcatccggca ttcggcacac gatgcctgat gcgacgctgg cgcgtcttat
    24421 caggcctaca ttggtgccgg atcggtagac cggataaggc gttcacgccg catccggcaa
    24481 gtggttaaac ccgctcaaac accgtcgcaa taccctgacc cagaccgata cacatcgtcg
    24541 ccagaccaaa ctgaacgtct ttgtgttcca tcagattcag cagcgtggtg ctgatacgcg
    24601 caccggaaca acccagcgga tgacccagcg cgatcgcgcc accgttgagg ttgatcttct
    24661 cgtcaatctg ctcaattagt cccagatctt taatacatgg caggatctgc gcggcaaagg
    24721 cttcattcat ttcaaacacg ccgatatcac tgacagaaag ccccgctttt ttcagcgcca
    24781 gcttcgaggc cggaaccggg ccgtaaccca tgatcgatgg gtcacaacca acgaccgcca
    24841 tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt tcactcatca
    24901 ccagcatggc agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt accgtaccgt
    24961 ttactggatc aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa
    25021 tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc atcgtgacca ccagttggga
    25081 tgatttcatt tttaaatgcg cccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg
    25141 cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc atttctgccg
    25201 ttaagcccat catgcccgcc gctttggcga cattgcggct caggccaggg tgaaaatcga
    25261 cgccgtgact catcggcaca tggcccatat gctccacgcc gccaaccaga catgcctgcg
    25321 catcgccggt catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca
    25381 agcgattgac agtaaccgcc gggacagagt gcggcacttc tgccagcagc gccgcgttac
    25441 gggcgatatt aaaaccctgc tccagcgtct gctgcacaca cccccagtaa atatcgtcga
    25501 gggccgccgc ttccagcgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga
    25561 gatcttctgc acgcacgtta cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa
    25621 ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc aggtcgccaa
    25681 ccggacgggc tggctcaacc gggggatagt acggttcgtt atgacgcgct ttattacgca
    25741 gaccttccgg cacttcgtac agcgggccga ggtgctgata ttgctgtgcc atatcgaggt
    25801 attttgcgct gccgagggtg tccagccagc ggaacgcgcc gccgtggaac ggagggaagc
    25861 ccaggccgta gaccagcgcc atatccgctt ctgccggagt ggcgataatg ccttcttcca
    25921 gacagcgcac cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc
    25981 tgaaatcgcg cttcggctgg ctcacttctg ccagcaggtc gtcaacagcg gcgtcttctt
    26041 ctttcttcgg cttacctttg ctgtcttctt tataatgcca gaaaccgagg ccgttcttct
    26101 gaccaaagcg gttggcatca aacagcgcgt cgatggcatc gcgataatct ttctgcatcc
    26161 gctgtgggaa gcccgctgcc atgactgcct gggcgtgatg cgcggtgtca atgcccacaa
    26221 cgtccagcag atatgccgga cccataggcc agccaaactg tttttccatc actttgtcga
    26281 tcttgcggaa atccgcgccg tcgcgcagca actggctgaa accggcgaaa tacgggaaca
    26341 gcacgcggtt gacgaagaag ccggggcaat cgttaaccac aatcggcgtt ttacccatct
    26401 tgctcgccca ggcgacgact ttcgcaatag tttcgtcgga gcttttctcg ccgcgaataa
    26461 tttccaccaa cggcattcgg tggaccgggt taaagaagtg catcccgcag aagttttccg
    26521 gacgttccag cgcgttggcc agttcgctga taggaatggt tgaagtgtta gacgccagca
    26581 cggtatccgg gcgtactttc tgctcggttt ctgccagcac ggcttttttc actttcgggt
    26641 tttcaacaat cgcttctacc acaacatcca cgcgctcaaa tccggcgtag tcgagcgttg
    26701 ggtggatcgt ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgctcaagct
    26761 gcttgttcag tagtttcgcg gcttcagtca tgccgagggc taacgacttg tcgttaatat
    26821 ctttcataat caccggcacg cctttccacg cagactggta agcgatgccg ccacccataa
    26881 tgcctgcgcc cagcacggcg gcctgtttcg gggtttcaac gtctttggtg agtttcttcg
    26941 ctttgccttt tacatattga tcgttaagga aaatgccgac cagtgcgcga gcttcgttgg
    27001 tatgcgccag cgggacaaag cttttgtttt ctaggtttaa ggcttcttca cgaccaaaac
    27061 gagccgcagc ttcaatggtt tttactgcgg tgatgggggc cgggtaatgt tttcctgccg
    27121 tttgtgcgac catcccttta gcgatggtga agctcatggt ggcttcaatc ttgctcagtt
    27181 ttagcggttc cagcttcggc tgacgtttcg ctttccagtc gaggtcgccg ttaatggcct
    27241 ggcgtaaaat cgccatcgcc ccttcaatca gtttttctgc tttgactacg ccatccacca
    27301 gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg atttccagcg
    27361 cactgtcagc gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc
    27421 ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacgg taatcggtcg
    27481 ccagcacgca ttcgcagcca ccgcccagcg catagccatt gacggcagca atggtcggca
    27541 ccggcagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact
    27601 gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg
    27661 ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga
    27721 tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt attaactgaa cctggggcat
    27781 caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt
    27841 aaagcatgtc agtctcctga atccgcaagg tgatctggtc gtaccagatg catcgaagtg
    27901 tgtattttgt gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct
    27961 cggcagaaat cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa
    28021 aacagaaggg taaaaaatgg aatcactggc ctcgctctat aaaaatcata tagctacatt
    28081 gcaggaacga actcgcgatg cgctgacgcg cttcaagctg gatgcgttac ttattcactc
    28141 cggcgaactg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca
    28201 attcaaagcg tgggtgccgg taactcaggt gccaaactgc tggctgctgg tggatggcgt
    28261 gaataagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg tcgaaccgct
    28321 gccgacctcc ttctggactg aagatgtaga agtgatcgcg ctgccgaaag ccgatggcat
    28381 tggtagtctg ttgcctgctg cgcgcggcaa tatcggttat atcggtccgg tgccggaacg
    28441 tgcgctgcaa ctgggtattg aggccagcaa tatcaacccg aaaggggtta tcgactacct
    28501 gcattactac cgctccttca aaaccgagta cgagctggcc tgtatgcgtg aagcgcagaa
    28561 aatggcggtc aacggtcatc gcgcggcaga agaagcgttc cgttctggca tgagcgagtt
    28621 cgatatcaat attgcctatc tgactgcgac cggtcatcgt gataccgacg taccttacag
    28681 caacattgtg gcactcaacg aacacgctgc ggtgctgcat tacaccaaac tggatcatca
    28741 ggcgtcggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc
    28801 cgctgacctg acccgtacct ggtcggcaaa aagtgacaac gattacgcac agctggtgaa
    28861 agacgtaaat gatgaacaac tggcgctgat cgcgaccatg aaagctggcg ttagctatgt
    28921 ggattaccac atccagttcc atcagcgcat cgccaaattg ctgcgtaaac atcaaatcat
    28981 caccgatatg agtgaagagg cgatggtcga aaacgatctt accgggccgt ttatgccgca
    29041 tggtatcggc catccgctgg gcctgcaggt gcatgacgtc gccggtttta tgcaggatga
    29101 tagcggtacg cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct
    29161 ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcattgaat cgctgctggc
    29221 accgtggcgt gaagggcagt tcagcaagca cttcaactgg cagaaaattg aagcactgaa
    29281 accgttcggc ggcattcgta tcgaagacaa cgtggtgatc cacgaaaata acgtggaaaa
    29341 catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc
    29401 acggtcgttg aagagatcaa aaaaagccgc ttcattacgc tgttggcgca taccgatggc
    29461 gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga tgcccgccac
    29521 cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac
    29581 gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat gggcagcggc
    29641 gttggggaaa ttaccgctgt ggtggtgcgc tactacggcg gcatattgct tggcaccggt
    29701 gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa
    29761 cgcaagacgc cattaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt
    29821 gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca ggcattcgtt
    29881 ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcgaa gctggcggat
    29941 tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca
    30001 gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg
    30061 tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg
    30121 gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggc tctatgctgt
    30181 ggtggccgaa ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt ctgatagtgg
    30241 tgctgttctg gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc tcggaaagcc
    30301 cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc accaccgggg
    30361 ccactacgct ggtggggctg gattcgctcc ctcatgccat cctcttttat cgccagatgc
    30421 tgcaatggtt tggcgggatg gggatcatcg tgttagcggt tgcgatactg cctatcctcg
    30481 gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa
    30541 tgcgcccgcg aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg
    30601 tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc ctttgatgcc atcggccata
    30661 gctttgcgac tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg
    30721 atagcccgac tattaacact atcattgcta tcttcctgct gatctccggc tgtaactacg
    30781 gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat
    30841 ttcgcatgtt tatcggcgta cagtttacgc tggtggttat ttgtaccctc gtactgtggt
    30901 ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtgt
    30961 cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg ctctttttgc
    31021 cggtactgct tttatgttca gcttttatcg gcggttgtgc cgggtcaacg ggcggtggcc
    31081 tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgaactg aaacgactgg
    31141 tgcatccgaa cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc
    31201 tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg
    31261 cgattatcgc cacgggcgtg gatgactttt ctgcctttgc ctcggttgtt gcgacattga
    31321 ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg aacccggtgg
    31381 ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg
    31441 tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct
    31501 tttctcaaca agggacggac aaacgcgcga gattgcctcc tacttggctt cggaactgaa
    31561 agaactgggg atccaggcgg atgtcgccaa tgtgcaccgc attgaagaac cacagtggga
    31621 aaactacgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc actctgcgtt
    31681 ccaggaattt gtcaaaaagc atgcgacgcg gctgaattcg atgccgagcg cgttttactc
    31741 cgtaaacctg gtggcgcgta aaccggagaa gcgcactcca cagaccaaca gctacgcgcg
    31801 gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg ccggggcgct
    31861 gcgttaccca cgttatcgct ggtacgaccg ttttatgatc aagctgatta tgaagatgtc
    31921 aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa
    31981 tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag cataaagaat
    32041 aaaaaatgcg cggtcagaaa attattttaa atttcccctt gtcaggccgg aataactccc
    32101 tataatgcgc caccactgac acggaacaac ggcaaacacg ccgccgggtc agcgggattc
    32161 tcctgagaat tccggcagag aaagcaaaaa taaatgcttg actctgtag
//
LOCUS       DAZFTN010000054        32168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFTN010000054 DAZFTN010000000
VERSION     DAZFTN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            234..1154
                     /gene="cbpA"
                     /locus_tag="ACHKV2_002650"
     CDS             234..1154
                     /gene="cbpA"
                     /locus_tag="ACHKV2_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO6940514.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1154..1459
                     /gene="cbpM"
                     /locus_tag="ACHKV2_002651"
     CDS             1154..1459
                     /gene="cbpM"
                     /locus_tag="ACHKV2_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO6940515.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1613..2212)
                     /gene="torD"
                     /locus_tag="ACHKV2_002652"
     CDS             complement(1613..2212)
                     /gene="torD"
                     /locus_tag="ACHKV2_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO6940516.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2209..4755)
                     /gene="torA"
                     /locus_tag="ACHKV2_002653"
     CDS             complement(2209..4755)
                     /gene="torA"
                     /locus_tag="ACHKV2_002653"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO6940517.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4755..5927)
                     /gene="torC"
                     /locus_tag="ACHKV2_002654"
     CDS             complement(4755..5927)
                     /gene="torC"
                     /locus_tag="ACHKV2_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO6940518.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6057..6749
                     /gene="torR"
                     /locus_tag="ACHKV2_002655"
     CDS             6057..6749
                     /gene="torR"
                     /locus_tag="ACHKV2_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO6940519.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6722..7750)
                     /gene="torT"
                     /locus_tag="ACHKV2_002656"
     CDS             complement(6722..7750)
                     /gene="torT"
                     /locus_tag="ACHKV2_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO6940520.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7833..10577
                     /gene="torS"
                     /locus_tag="ACHKV2_002657"
     CDS             7833..10577
                     /gene="torS"
                     /locus_tag="ACHKV2_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO6940521.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10649..11722
                     /gene="yccM"
                     /locus_tag="ACHKV2_002658"
     CDS             10649..11722
                     /gene="yccM"
                     /locus_tag="ACHKV2_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6940522.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11771..11905)
                     /locus_tag="ACHKV2_002659"
     CDS             complement(11771..11905)
                     /locus_tag="ACHKV2_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO6940523.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(11933..12163)
                     /gene="ymcE"
                     /locus_tag="ACHKV2_002660"
     CDS             complement(11933..12163)
                     /gene="ymcE"
                     /locus_tag="ACHKV2_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO6940524.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12138..12326)
                     /gene="ymcF"
                     /locus_tag="ACHKV2_002661"
     CDS             complement(12138..12326)
                     /gene="ymcF"
                     /locus_tag="ACHKV2_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6940525.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12337..12549)
                     /gene="cspG"
                     /locus_tag="ACHKV2_002662"
     CDS             complement(12337..12549)
                     /gene="cspG"
                     /locus_tag="ACHKV2_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO6940526.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12835..13047
                     /gene="cspH"
                     /locus_tag="ACHKV2_002663"
     CDS             12835..13047
                     /gene="cspH"
                     /locus_tag="ACHKV2_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO6940527.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13489..13794
                     /locus_tag="ACHKV2_002664"
     CDS             13489..13794
                     /locus_tag="ACHKV2_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940528.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            13901..14545
                     /gene="gfcB"
                     /locus_tag="ACHKV2_002665"
     CDS             13901..14545
                     /gene="gfcB"
                     /locus_tag="ACHKV2_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO6940529.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14542..15288
                     /gene="gfcC"
                     /locus_tag="ACHKV2_002666"
     CDS             14542..15288
                     /gene="gfcC"
                     /locus_tag="ACHKV2_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6940530.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15288..17384
                     /gene="gfcD"
                     /locus_tag="ACHKV2_002667"
     CDS             15288..17384
                     /gene="gfcD"
                     /locus_tag="ACHKV2_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6940531.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17430..18569
                     /gene="gfcE"
                     /locus_tag="ACHKV2_002668"
     CDS             17430..18569
                     /gene="gfcE"
                     /locus_tag="ACHKV2_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO6940532.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18557..19003
                     /gene="etp"
                     /locus_tag="ACHKV2_002669"
                     /gene_synonym="yccY"
     CDS             18557..19003
                     /gene="etp"
                     /locus_tag="ACHKV2_002669"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO6940533.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19023..21203
                     /gene="etk"
                     /locus_tag="ACHKV2_002670"
     CDS             19023..21203
                     /gene="etk"
                     /locus_tag="ACHKV2_002670"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO6940534.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21323..22627)
                     /gene="appA"
                     /locus_tag="ACHKV2_002671"
     CDS             complement(21323..22627)
                     /gene="appA"
                     /locus_tag="ACHKV2_002671"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO6940535.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(22707..22799)
                     /gene="cbdX"
                     /locus_tag="ACHKV2_002672"
     CDS             complement(22707..22799)
                     /gene="cbdX"
                     /locus_tag="ACHKV2_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO6940536.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(22812..23948)
                     /gene="appB"
                     /locus_tag="ACHKV2_002673"
     CDS             complement(22812..23948)
                     /gene="appB"
                     /locus_tag="ACHKV2_002673"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6940537.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(23960..25504)
                     /gene="appC"
                     /locus_tag="ACHKV2_002674"
     CDS             complement(23960..25504)
                     /gene="appC"
                     /locus_tag="ACHKV2_002674"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO6940538.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25638..26495)
                     /gene="hyaF"
                     /locus_tag="ACHKV2_002675"
     CDS             complement(25638..26495)
                     /gene="hyaF"
                     /locus_tag="ACHKV2_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO6940539.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26492..26890)
                     /gene="hyaE"
                     /locus_tag="ACHKV2_002676"
     CDS             complement(26492..26890)
                     /gene="hyaE"
                     /locus_tag="ACHKV2_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO6940540.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(26887..27474)
                     /gene="hyaD"
                     /locus_tag="ACHKV2_002677"
     CDS             complement(26887..27474)
                     /gene="hyaD"
                     /locus_tag="ACHKV2_002677"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO6940541.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27471..28178)
                     /gene="hyaC"
                     /locus_tag="ACHKV2_002678"
     CDS             complement(27471..28178)
                     /gene="hyaC"
                     /locus_tag="ACHKV2_002678"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO6940542.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28197..29990)
                     /gene="hyaB"
                     /locus_tag="ACHKV2_002679"
     CDS             complement(28197..29990)
                     /gene="hyaB"
                     /locus_tag="ACHKV2_002679"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO6940543.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(29987..31105)
                     /gene="hyaA"
                     /locus_tag="ACHKV2_002680"
     CDS             complement(29987..31105)
                     /gene="hyaA"
                     /locus_tag="ACHKV2_002680"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO6940544.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31531..31618
                     /locus_tag="ACHKV2_002681"
     tRNA            31531..31618
                     /locus_tag="ACHKV2_002681"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31565..31567,aa:Ser,seq:tga)
BASE COUNT         7848 a         8489 c         8222 g         7609 t
ORIGIN      
        1 taataacgca ctagtaaaac ttaaagtgcc tgtttcaact atcaaaaatc gctcaccctt
       61 tttcacctgt ttaaaatatg ttcagcgccc caccttgagg cgacctcctc tccgcgatga
      121 tttcaataac atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg
      181 agttacctta caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat
      241 taaaggatta ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga
      301 ccgcctatcg tcgacttgcc cgcaaatacc atcctgatgt cagcaaagaa ccggatgccg
      361 aagcccgctt caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg
      421 ctgagtatga tcagatgtgg caacatcgca acgatccgca atttagccgt cagttccagc
      481 atggcgacgg tcagagtttt aacgccgaag attttgacga tatcttctcg tcaattttcg
      541 gtcagcatgc ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg
      601 aagtggcggt attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc
      661 tgccggttta taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga
      721 agatcccggc aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg
      781 gcgaaaacgg cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc
      841 tgtttgatat tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg
      901 cgctgggtgc taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc
      961 ctggcagcca ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac
     1021 agaccggcga tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca
     1081 ctgccgcgct gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt
     1141 gggggaaagc ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca
     1201 taccggcatc tctgaagagg aattgaatga aattgtcggt ttgggggtgg ttgaaccgcg
     1261 tgagatccag gaaacaacct gggtatttga cgaccatgcc gccatcgtgg tgcaacgcgc
     1321 ggtgcgcctg cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt
     1381 aatggatgat attgcgcacc tgaagcagga aaaccgcctg ctgcgccagc ggctttcccg
     1441 gtttgtagct catccgtgat gtgtgtttgc cggatgtgat gacgcatctt agcctgcctg
     1501 caaagagtac ttttcctgtg ggccggataa ggcgtgttca cgccgcatcc ggcggcggtg
     1561 ccctaatgcc tgatgcgacg ctaaacgcgt cttatcaggc taacaaacga cgttatctgt
     1621 tttggtggtc gccctcaact aacaccagca ataactggct tagtgccgcg taaaaaccaa
     1681 agctgtcata ctgatggcaa cgcgcaacaa actctggtaa ccattgccgc aacgccgtga
     1741 gtgttttttg ccgtaaactg tcgattcttc gcgcaggaac cgtcccctct cccagcgaaa
     1801 aatgcagatg actcaacaac tcgagataga tcgccagatg atctgtcggt tcgttgaaat
     1861 tgccgctggt ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt
     1921 tgtaggccga tgcatacggc agcgccgctt gtttgtcagt catcagaaac aggccgcaaa
     1981 agtcagcggc cagttccaga cgggcatcgt cacgtactgt cagagtggca acacggtttt
     2041 ccagctcatc caccgccgca gtgagcggcg gttcgctttt cagcaacgaa aaccattcag
     2101 ccatctgcgc actggcgatt tgcgtcagtt gttcatcgtc cagttcacgg gagaacaact
     2161 gcgccagcca ggcgtaaaca caggctatct gttgtgctgt cagcgtggtc ataatttcac
     2221 ctgcgacgcg ggaacatatt cgcactgcgc caccatctcc acggggccgt taaacgccgt
     2281 tacctgctcc actgttccgt tgcacttctc aatttccacc agcgtagtgt gcgcactggt
     2341 cgcctgcgcc agctgcgaag taccgatgtc aatggtcaac acgttggggt taccgtattt
     2401 gcacagcgca ccaggctcgc cgcctttatc tggatcgtgc catgcccctt cgtgaattcg
     2461 cgccacgccg ggtgcatagc ggtcagaaac tactgcccct gccaacacct gaccgcgagc
     2521 gttaaagacg cgtaccacat caccgttacg aataccgcgc gcgctggcat cctgcgggtt
     2581 aatgaacact ggctctttac ccgctaccgt atattgctga cgcagagttt ccgactcaca
     2641 taactgcgag tgaagtcgga aatccggatg cacagattgc agatgcaacg gatacgtctg
     2701 cgagccgggc ccaccgtggg agcgttcgat tttctcaaac cacatcggat gcccctgaca
     2761 atcgtcgtag ttcatatcgg cgatggtttt cgaatagatc tcaatcaggc cactcggcgt
     2821 gcccagcggt tcgagatctg gatcttcgcg gaatgcctgg tggcgaacaa acatctgcgg
     2881 atggtcaaac tcgacgtact ctttgttatt ccagaagtca tcaaacgctg gcaaatgaac
     2941 gccgcgtcct ttgccttgct gaacaccttc ctgccagatg cgtttcagcc agcccatttc
     3001 gtccagccct tcggtaaagg cttcttcgcg attaaagcgg cggcacagct cgcggaaaat
     3061 atcaaagtcg ttgcgcgcct cgaactgcgg cggcacgacc tgtttcatgg cgataatgcc
     3121 acggttggag tggttgccgt actggtcgag atcgttacgc tcaaactgcg tggtcgcagg
     3181 cagcacgata tcggcaaagc ggcaggttga ggtccactgg ttatctatgg cgataaccgt
     3241 ttccagtttg cgccagcctt caataatgcg gttgatctgc tgatggcgat ggaaggggtt
     3301 agttccggca aaaatacaca ttttcagcgg cggcagtttt accgatttac cgttccagtt
     3361 gattactttc cccggttcga ggatcgcatc gataaaacgg gcaatcggaa tggtgctgct
     3421 gtagcctttg taatcactgt tgtcgtgaac aggcggaatc gacgtagagc cggagaaacc
     3481 actcagaata acgcctttac gccccggcgt gcctgcgccg ttatagtgcc agccaaaacc
     3541 aaagccacca cctggcaggc caatttgccc cagcatcgcc gccagaacca caatcatcca
     3601 cgcccactgt tcaccgtgct gcatacgctg tacgcaccag ccagcaataa tctgcgttct
     3661 gttcgccgcc atctgccgcg ccagtccacg aatggtttcg gcatcaatgc cggtcagttt
     3721 ttcagcccat gcggcatctt tcggctgacc gtctttctcg cccagcagat acggcaggaa
     3781 ctgctcaaaa cccacacagt agttagcgag gaagtttttg tcgtacaggt tttcactgta
     3841 caacgtatgc gccagcgcca gttgcagcgg cacgtcagtt tgcgggttaa ccgcaatgtg
     3901 cttcacatgc tcgcgcccca gatactcatg ggtggatgta acaaccggat cgatgctaat
     3961 gacctcaatt tcaccggcgg cgactttcgc tttcaactgc gcgtaatatt cataaacatc
     4021 gtgatccggg caccaccagt tcgcttgctg gtttttcagc aaatcggagc cccacagcac
     4081 aatggttttg ctgttctgca ataccagcgg ccaggaggtt tgctgttcat acacttccat
     4141 cgaaccgact acgcgcggca ggatcacctg cgcagcacca gtagagtaat ctccgcccgt
     4201 accaacgcta ttaccatgca aggcaatagc tttcgccagc atccccgaag cgttatggaa
     4261 catccccgtc gattgccaac cactggcggt cagcaaggca ctcggcccgt gagttttctg
     4321 tacgcgttcc agttcttcat agaacatgtc gagggcttca tcccagctca cacgcacaaa
     4381 acggttatca ccgcgctggg aggtgtcgct cagatggcgc ttacgcagcc agtccacgcg
     4441 taccatcgga taacgaatac gcgccgcgtt gtgtacatga tccggcaatc cggcaatcat
     4501 tttcgacgga tatttatcca gttcgaacgg ttttgccgcc acaaagcgac catccttcac
     4561 cgtcgcgcgg atagcccccc agtgcgaccc ggtaagaatg ccctctttcg agatgacagc
     4621 ctcagtcgcc gcttgcgccg cagtcgcacg gcgcgacgtt aacaatgacg gccccagcat
     4681 cccggcgacg gttaagccgc cgagttgtgc cagaaaacgc cgacgtgatg cctgaaagag
     4741 atcgttattg ttcattattt ttcttccttc ttatcgccgt gagccttacc tgcggtgtca
     4801 gacgcattca tttgcagata tttcaacaag gtgcgttctt cacgtttatc gaggctggta
     4861 aagccgatca tgccgttgag cgtgccaatc cagccgttag cgtcaaagtg agagatttcc
     4921 ggtgcgccgt ggcactggtt acaggtgccg ttgtacaacg aatccgcata agcccagatc
     4981 ggtttgatat cgttcaccat gtcacctttc ttcatccacg cggtggcctg caacttgctc
     5041 cactcggtat tggtgtcggc aacggtggtt ttctccagcg tttttacctg ctgctgcaca
     5101 tcaccacgaa tcgaggcaac aaagatgcgt ttacctggga attgggtgag tacacgctga
     5161 cgtccggcgc tttctgtcca gccggtaatt tcaatttgca gccagtcgcc gtcacgttta
     5221 aggactttca cttccgaggc aggcagcaga gaaccagagg cttctttatc gcctttcgcc
     5281 gcataaattg gcttaatatc aatagagtac agcgtgtcac cactgtcatt agcaccggcg
     5341 cgcagctcat cgaactgctt acggaagccg ctactcatat ccggtaactg gtgggcaata
     5401 cctttatgac agtcgatgca ggattgatta tctttcgctg ccaccttcat ctgacgtgcc
     5461 gcttcaggat gctgcttcgc atgatccatc gcatcgtagt tatggcagga gcgacaggtt
     5521 gccgagttgt tttctttcat tcgcgcccat tcacgctcgg caagttccgc gcgtttggct
     5581 tcaaattttt caggtgtatc aatggagtgg gcaataaagg tctggtagat atcgttgctc
     5641 gcttccagtt tgcgcttcac catgcctgga atatccggcg ggatatgaca gtcatggcat
     5701 tcagctcgca cgccggaggc gttctggaaa tgcaccgact gtttatattc ttcatacact
     5761 ggttgcatac tgtggcaact gacacaaaat tcggttgtgc tggtgacttt gatcccaacg
     5821 tgtggcaata caatcagcgc aatgccaatc acaatcccaa tagcgaccag cgccagtacc
     5881 gaccaacgag cactgggtcg gcgtagcgcg ttccagagtt tccgcataat agcccctgta
     5941 aaattatggt ttagtgaagc gatcttaatg agtaaatatg aacagcggca ctggtcagga
     6001 tgaacggctt acggcagaat atgaacagat atgaacagaa tgagtaaaac cctctgatgc
     6061 cacatcacat tgttattgtt gaagatgagc cggttaccca ggcgcgatta caatcctact
     6121 tcactcagga ggggtatacc gtttccgtta cggcgagcgg tgccgggcta cgggaaatta
     6181 tgcagaatca gccggtggat ttaattctgc tggatatcaa cttgcccgat gaaaatggcc
     6241 tgatgttaac ccgcgccctg cgagaacgct caacggtggg gattattctg gttaccggac
     6301 gcagcgatcg gattgaccgt attgttgggc tggaaatggg cgcagacgat tacgtcacca
     6361 aaccgctgga actgcgcgaa ctggtagtac gggtaaaaaa tctgctctgg cgaatcgacc
     6421 tcgcgcgaca agctcaaccg tacactcagg acaactgcta tcgctttgcg ggttattgcc
     6481 tgaatgtgtc gcgccatacg ctggagcggg atggtgagcc gattaaactg acccgcgcag
     6541 agtatgaaat gttggtggca tttgtgacca atccgggcga aattctcagc cgtgaacgtc
     6601 tgctgcgtat gctctctgcg cgtcgggtgg aaaaccctga cctgcgcacc gtcgatgtgt
     6661 taattcgtcg tttacgccat aaactcagcg cggatttact ggtgacgcaa catggcgaag
     6721 gttatttctt agccgctgat gtgtgctgat aaaaatagac cggacgaaat cccccaggcg
     6781 acagcgagcg gcggatatgt tcgcggtcgg catttttcgg cgtcagaact aaaatcggtg
     6841 ggctgacatt atcagacacc gattgcccct gtaattgcct gatggcctgc tcaactgcca
     6901 gttccccctg ccagaccatt tgatcgctgg cagccataat cactcttccc cgcttcagcc
     6961 cgcgatacac ctgatgtgaa agataaaacg acaccacggt aagcggcgtt ttcaggttac
     7021 gcccttcccc cattgccgcc tctgccgcaa tggccgttcc ggcaacgacg tcgatttctg
     7081 gatggcgttc cagcatctcc tgcaacaggt tacgctggat ttcaatatcg ttatcaccaa
     7141 gcgcaatatc aacaatacgc accgggcttc cggcaatggc tgcgcgaaaa ccctcgacca
     7201 tctccttact gcccccggcg ttatcgggtc cgggcatcaa cagcacattc agtggtttac
     7261 cgtgcgccca ttgcaccaga tatcgcccag gttgatagcc catctgaaac cagggcacac
     7321 caacgcggct tttcacctgg ggagcatcaa tagcatttac cagctcgatc accggcagat
     7381 ttgctacttg cttttgcaga tcgggaaatg acgtcgtgct actaccgagc aaaatggcct
     7441 ctgcgcccca ctgtttacac tggtcgattt gtgcttgctg ggtagccaac tggctgtagc
     7501 cgcctgcctc cagcactttt aaatccacac cgtagcggcg agcagcctcc tgcataccat
     7561 agttcaacga taaccaatat gaatctttca ggctgggata aagcgcgcac agtttccata
     7621 cgcgtttggc ttttagcggc atagaggctt gcaccgtgaa atgctgcgca tcatgccagc
     7681 gcaacaggtt atcagccgaa aatgccggca acatgaaaag ggaaagaagt aaaaatagca
     7741 gcacgcgcat gatagcctca tcaataataa ggctttatgc tagatgcatt ccgctttgcg
     7801 actcaacatt tttcacctta agtgcaccga ccgtgaattt aaccctgacc cgaagactct
     7861 ggatgggctt tgccctgatg gcgctgttaa ccctgaccag taccctggtg ggatggtaca
     7921 acctgcgctt tatcagccag gtggaaaaag acaatactca ggcattgatt cctaccatga
     7981 atatggcgcg ccagttgagc gaagccagcg cctgggaact tttcgccgcg cagaacctga
     8041 ccagtgccga taacgaaaag atgtggcagg cgcaggggcg aatgctcacc gcacaaagcc
     8101 tgaagattaa tgcgttgctg caagcgttac gggaacaagg ttttgatacc accgctattg
     8161 aacaacagga gcaggagatc tcccgttcgt tacgtcagca aggggaactg gtggggcggc
     8221 gtctgcaact gcgccagcaa caacagcgac tcagtcagca gatagtcgct gccgccgatg
     8281 agatcgcacg cctggcgcaa ggtcaggcga ataatgcggc aacctccgcc ggagcgaccc
     8341 aggccgggat ttacgatttg atcgaacaag atcagcgtca ggctgctgaa agtgcactcg
     8401 atcggctgat tgatatcgat cttgagtatg ttaaccagat gaatgaactg cgccttagcg
     8461 ctctgcgggt gcagcaaatg gtgatgaatc tggggctgga gcagatccag aaaaatgcgc
     8521 caacgctgga aaagcagctc aataatgcgg tgaaaattct gcaacgtcgg caaatacgca
     8581 ttgaagatcc gggagttcgt gctcaggtcg caacaacgtt aactaccgtt agccaatata
     8641 gcgatttgct ggcgctgttc cagcaggaca gtgaaatcag caatcaccta caaactctcg
     8701 cacaaaataa catcgcccag ttcgcgcagt ttagcagcga agtcagtcag ctggtcgaca
     8761 ccattgagct gcgtaatcag cacggactgg cgcatctgga aaaagccagt gcacgcgggc
     8821 aatacagcct gttattgctg gggatggttt cactttgcgc actgattctg atcctctggc
     8881 gcgtggttta tcgctcagtc acgcgtccac ttgccgaaca aacgcaggcg ctgcaacggc
     8941 tgctggacgg tgatattgac tcccctttcc cggaaaccgc tggcgtacgg gagctggaca
     9001 ccatcgggcg gctgatggat gcgtttcgca gcagtgttca tgcactgaat cgccaccgcg
     9061 aacagctggc ggcgcaggtc aaagcgcgta cagctgaatt gcaggaactg gtgatagaac
     9121 accgacaagc acgggcggaa gcagaaaaag ccagccaggc aaaatcggcg tttctggcgg
     9181 cgatgagcca tgagatccgc acaccgctgt acggtattct cggcaccgca cagctactgg
     9241 cagataaccc cgcacttaac gcccagcgtg atgatttgcg ggcaattacc gactccggcg
     9301 aatcgttgct gaccatcctc aacgatattc tcgattattc cgctatcgaa gcaggtggca
     9361 agaatgtttc ggtcagcgat gaaccctttg aaccgcgacc gctgctggaa agtaccctgc
     9421 aattaatgag tggacgggtg aaaggtcgcc cgattcgcct ggcaacggca attgccgacg
     9481 atgtaccgac tgcgttaatg ggcgatccgc ggcgtattcg tcaggtcatt accaacctgt
     9541 tgagcaacgc cctgcgtttt actgacgaag ggcagatcgt tttacgtagc cgtactgatg
     9601 gtgagcaatg gctggtcgaa gtggaagaca gcggctgcgg tattgatccc gcgaaactgg
     9661 cagaaatctt ccagccattt attcaggtaa gtggcaaacg cggcggcacc gggctggggc
     9721 tgactatcag tagccgtctg gcccaggcga tgggcggcga actgagcgcc accagcacgc
     9781 cggaggttgg aagctgtttt tgtttacgct tgccgttacg tgttgccacg gcacccgtgc
     9841 caaaaacagt caatcaggcg gtgcgtcttg acggtttacg tttgctgtta attgaagata
     9901 acccgctaac ccagcgaatt accgttgaga tgctgaacac cagtggtgcg caggttgttg
     9961 ctattggcaa tgccgcgcag gctttagaga cactgcaaaa tagcgaaccg tttgctgccg
    10021 cactggtgga ttttgatctg ccggatgtca acggcattac gcttgcccga caactggcac
    10081 gacaatatcc gtcgctggtt ttgattggct ttagcgccca tgtcattgac gaaacgctgc
    10141 gccagcgtac cagttcacta tttcgcggga ttatccctaa accggtgccg cgtgaagtgc
    10201 tcggtcagtt actggcgcac tatctccaac tgcaagccaa taacgatcta ccactggatg
    10261 tatcgcaact caatgaagac gctcagttaa tggggacgga gaagatccac gaatggctgg
    10321 cattatttac acaacatgcc ctgccgcttc tcgatgaaat cgacattgcc cgcgccactc
    10381 aggatagcga gaaaataaag cgtgccgcgc atcagctaaa aagcagttgc tcgagtctgg
    10441 gaatgcgtag cgccagccag ctctgcgcac aactggagca gcagccatta tctgcccccc
    10501 ttccacacga agaaattaca cgcagtgttg ccgctctgga agcatggttg aataagaaag
    10561 acctgaacgc gatttgaatc aatatcagcc agttaagaat aactagaata gtggcatctg
    10621 gtatgcaact taacggactg gcaaaatcat ggcagagaaa aaaagaaccc gctggcagcg
    10681 gcggccaggc acgacgggcg gcaaattacc gtggaatgac tggcacaatg ccacgacctg
    10741 gcgtaaagcg acgcaattat tgctgttggc aatgaatatt tatattgcca tcacgttctg
    10801 gtattgggtg cgctattacg aaacggcagg tagtacgata tttgtcgcca gaccgggagg
    10861 tatcgaaggc tggctaccga ttgccggtct gatgaatctg aaatatagcc ttgcaacagg
    10921 tcagttaccg tccgtccacg ccgccgcgat gctgttattg gtcgctttta tcgtcatcag
    10981 tctattactc aaaaaggcct tttgctcatg gttatgcccg gttggtacgc tttctgaatt
    11041 aatcggcgct ctcggtaaca aactgtttgg tcggcaatgt gtccttcccc gctggctgga
    11101 tattcctctg cgcggtgtaa agtatttgct gttgagtttt tttctctata tcgcgttatt
    11161 gatgcctgct caggcgattc actattttat gttgtcgccc tacagcgtgg tgatggacgt
    11221 taaaatgctc gatttctttc gtcatatggg gaccgcgaca ttaatcagcg tgaccgtttt
    11281 gctgattgcc agcctgttta ttcgccatgc ctggtgtcgt tatctttgcc catatggcgc
    11341 gctgatgggc gtggtttcgc tattatcgcc gtttaagatt cgtcgcaatg ccgaaagttg
    11401 tatcgattgt ggcaaatgcg caaaaaattg cccatcacgg atcccggtcg ataaattaat
    11461 tcaggtacga acagtggaat gtaccggctg tatgacttgc gtagagtcat gtccggtagc
    11521 ctcaacattg accttttcac tgcaaaaacc tgcggcaaat aaaaaagcct ttgcgttgtc
    11581 tggctggtta atgacgctac tggttctggg gattatgttt acggtgattg gttacgcaat
    11641 gtatgcagga gtatggcaaa gcccggtgcc ggaggaattg taccgacgct taattccgca
    11701 agcgccaatg attggtcact aaattagcaa caagcggaac atcacctcgc acggggtgat
    11761 gttcacaaaa tcacattatt ttgattttga catcataact ttcaagcccg gtcatttttt
    11821 ctcgcgcggt gaagcgaatt tcagagactt ctttccctgt attcttgttc agctcggcga
    11881 ttttttgcgc gataaagtcg gcgatttccg tttcggcttg ttttttaact cttcaatatt
    11941 cactgttaac ctcttctggt cttaaaagac agcaacacgc cagtaaggtg ttggttcccg
    12001 gtaccctggt caggtaagaa atgttataag ccatctcccc gaaagattca ataatcaaca
    12061 ggttatcgct tactatacat acagcgttga aaaaaaataa gacagatata aaaaaggctc
    12121 cacaagggag cctgatatta ttttgtgaaa tccagcggtg catggtcagt gcgtatttgc
    12181 agcgcgacgt tatcaaaggt gatcatcgtc gatttacaaa aaatacactt agcgcccaaa
    12241 gggtttcgct ccgtcacatc gaatgccgat gtgcggtact gtgaaccatg acaacatgga
    12301 cagcgaaaat ggagatgttg agtaataacg gcaaccttag agcgtaacta cgttcgctgc
    12361 cgcggggcca cgttgaccct gctcaataga aaattcaact ttctgatttt cgttcagcgt
    12421 gcggaattca ttgctctgga tggcggtgaa atggacgaaa acgtctttgc tgccatcatc
    12481 aggagtgata aagccaaaac ctttatctgc gttaaaccat tttactaaac cagtcatttt
    12541 attagacata ttttacttcc ttcattttgg gcctttcggc aaacagggct ttaatacaga
    12601 aactacttag tgttcaggag agagactcaa aagaagggga taatgcctga taatgagaac
    12661 tgctttagta aactactttg tatgctgtct gtctttccaa ccgacgcagc tattaacgca
    12721 tgataattga gattaagcaa attttatttt agccgtccgg cggtcttaaa tatcgctggc
    12781 tttattttat taaccccgta tagtgcagcg cagtattttc agtaaggaat ttgtttgtcc
    12841 cgtaaaatga caggaattgt caaaaccttt gatcgcaaaa gcggcaaagg attcattatc
    12901 ccctccgacg gtcgtaaaga agttcaggtc catatttccg cattcactcc ccgcgacgca
    12961 gaagtgctta taccaggatt acgcgtggaa ttttgccgcg taaatggcct gcgaggacca
    13021 acagcggcaa atgtttatct ctcttaatat gacgctgctt gtttaaagct gactggtttt
    13081 attggcatag ttcaatagtc aacaccatgt attataacac aagttacttt ttcaccggaa
    13141 aactaatcca tttaaccgaa tatctctaca actaatagtt ataaatgcca cttaacagcg
    13201 agttatagta ccacttgaaa ctatattgcc aacaatagtt aatagctttc ttaacaattt
    13261 tcttatcttt aatttgtatt ttgctaagat ttatcttatg ctcacaggtc gtatttccag
    13321 aggggaatag ttaacaatct tatatttccc tctgcgcgcc tttctcaccc gctgtatata
    13381 gtgcaagtgg tatgaggaaa gggagatttc acaaggatgt tatactcaaa gtagacaatc
    13441 ctgatctcaa gcgtacgtat tgtccatgcg atgaataaag gaaaagttat gaaacacaag
    13501 ctttctgcaa tccttatggc cttcatgttg acgacgccag ctgcgtttgc tgcccctgaa
    13561 gcagcaaacg ggaccgaggc aacgacgggt actactggca ccacaacgac cactaccggt
    13621 gcaaccacga cggctgctac cactggtggc gtggctgctg gcgctgtcgg cacggcgaca
    13681 gttgtcggtg tggcaacggc tgttggtgtg gcgacgttgg cagtagttgc agcaaatgac
    13741 agtggcgatg gcggttctca taacacctca accaccacgt ccactacgcg ctaattctgc
    13801 ttaaacgcgt cgaaattaaa gttaaataat acccggttgc gatattcgta ccgggcttta
    13861 gagatttgca tttttttaat taacctttca aggattaaag gtgcgccctc ttattttatc
    13921 gattttcgca ctatttcttg cgggatgtac gcacagccag caaagtatgg tcgatacatt
    13981 tcgcgccagc cttttcgata atcaggatat caccgtagcg gatcagcaga tccaggcgtt
    14041 gccttattcc acgatgtatt tacgccttaa tgaagggcaa cgaatctttg tggtactggg
    14101 atatatagaa caagaacaaa gcaaatggtt atcccaggat aacgccatgc tggttaccca
    14161 caatggacgt cttttaaaga ccgtcaaact taataataat ctgctggaag tgacaaattc
    14221 cgggcaggac cctctgcgta acgcgctggc aataaaagac ggcagccgct ggacgcgcga
    14281 tattctctgg agtgaagaca accattttcg ctctgcgacc ctgagttcca ctttttcctt
    14341 tgctggatta gagacgctgc atattgcggg tcgcgatgtg ctgtgtaatg tctggcagga
    14401 agaggtgact tccacgctac cagaaaaaca gtggcaaaac acattctggg tcgattcggc
    14461 tactggccag gttcgtcaaa gtcggcaaat gttaggcgca ggggttattc ccgtagaaat
    14521 gacgtttctt aaacccgcac catgaataaa ttacagtcgt atttcattgc cagcgtactt
    14581 tacgtaatga caccccatgc ctttgcgcaa ggaacggtga ctatttatct gcctggcgaa
    14641 caacagacac tttccgttgg ccccgtggag aatgttgccc agttagtgac acaaccgcaa
    14701 ctgcgcgatc gcctctggtg gcctggtgcc ctgctgaccg actccgcggt gaaagctaaa
    14761 gcgcttaaag actaccaaca tgtcatggca cagcttgctt cctgggaagc agaagctgac
    14821 gacgatgtgg cggcaacgat taaatccgtg cgccagcagt tactcaatct caatattact
    14881 ggtcgcttac cggtcaaact ggaccctgat tttgtacgag tggatgagaa cagcaatccc
    14941 ccgctggtcg ggaactatac gctctatacc gtacagcgcc cggtgactat tactcttctg
    15001 ggtgcggttt ctggggctgg gcaattgccc tggcaggcag gccgtagcgt taccgattat
    15061 ctgcaagatc acccccgtct tgcaggcgcg gacaagaata acgttatcgt gattacgcct
    15121 gagggtgaga ctgtcgtcgc ccctgttgcc ctgtggaata aaagacatgt ggaaccaccg
    15181 cctggcagcc agctttggtt aggcttttct gcgcatgtac tgcccgaaaa atatgctgat
    15241 ttaaatgacc agattgtgtc tgttttaacg caacgtgttc ctgactgatg aagaagaatt
    15301 cttatctttt aagctgcctg gccattgccg tctccagtgc ctgtcatgct gaagtattaa
    15361 cctacccgga tccgctgggt tcgtcgcaat cggactttgg cggcacagga ttgttgcaga
    15421 tgccaaatgc gcgcatcgca ccggaaggtg aattcagcgt caactaccgg gataacgatc
    15481 aataccgttt ctactccacc tccgtggcgc tgttcccatg gctggaaggc accattcgtt
    15541 atacggatgt gcgcacacgt aaatatagcc agtgggaaga tttcagcggc gatcagtcat
    15601 acaaagacaa atcattcgat tttaaacttc gcctgtggga agaaggttac tggctaccgc
    15661 aagtggcgtt tggtaaacgt gatattgctg gtacgggtct gtttgacggt gagtatctgg
    15721 tggccagcaa gcaagcgggg ccatttgatt tcaccctcgg gatggcatgg ggctacgccg
    15781 gtaatgcggg caatattacc aacccgtttt gccgggtgag cgataaatat tgtcatcgcg
    15841 cagagtctca cgatgcgggc gatatcagct ttagcgatat ctttcgtggc ccggcttcca
    15901 tctttggcgg cattgagtat caaacgccgt ggaatcccct gcgtctgaaa ctcgaatacg
    15961 acggcaacaa ttaccagaat gatttcgctg gcaaactgcc tcaggcaagc catttcaacg
    16021 tcggtgcagt ttatcgcgct gccagctggg cagacctcaa cctgagttat gaacgcggta
    16081 acacgttgat gtttggtttc actttacgga ccaatttcaa cgatctgcgc cctgccctgc
    16141 gcgatacgcc aaaaccggca tatcaacctg cgcctgaatc tgaaggattg cagtacacca
    16201 cggtagcaaa ccaacttacc gccctgaagt ataacgcggg ctttgacgcg ccagaaattc
    16261 agctacgcga taagacgctg tatatgtctg gtcagcaata caaataccgt gactctcgtg
    16321 aagcggtcga tcgtgccaac cggattctgg tgaataacct gccgcaaggc gttgagaaga
    16381 ttagcgtgac gcaaaagcgc gagcatatgg cgatggtgac taccgaaacc gacgtagcca
    16441 gcctgcgcaa acagctggca ggtacagcgc ctggtcaatc agagccactg caacaacaac
    16501 gtgttgaagc tgaagatctt tctgcctttg gtcggggcta ccgtattcgt gaagatcgct
    16561 ttagctactc tttcaaccca acactttcac agtcgctggg cggcccggaa gatttctata
    16621 tgttccagct ggggctgatg tccagcgccc gctactggtt taccgaccac ctgctgcttg
    16681 atggcggtat tttcaccaat atttacaaca actacgacaa gtttaagtct tcgctgttgc
    16741 ccgcggactc taccctgccc cgcgtgcgca cgcatatccg tgattacgtt cgcaatgacg
    16801 tttatctcaa caacttgcaa gcgaattact ttgccgactt aggcaatggt ttctatggcc
    16861 aggtgtatgg cggttatctg gaaacgatgt atgccggtgt cggttccgag ctgctttatc
    16921 gcccgctaga tgccagctgg gcgctgggtg tggacgttaa ctacgtgaag cagcgtgact
    16981 gggacaacat gatgcgcttc accgattatt ccacgccaac tggtttcgtg acggcttact
    17041 ggaacccgcc gacgctcaat ggcgtactga tgaaacttag cgttgggcaa tatctggcaa
    17101 aagataaagg ggcaacgatc gacgtcgcca aacgctttga cagcggcgtg gcggtagggg
    17161 tatgggcggc aatcagtaac gtatctaaag atgactacgg cgaaggcggc tttagtaaag
    17221 gtttttatat ctcgattccg ttcgacttga tgaccattgg acctaaccgc aaccgcgcgg
    17281 tggtttcgtg gacaccattg acgcgtgatg gtggacaaat gctgtcacgc aaataccagc
    17341 tctatccaat gacggcagag cgagaagtac cggttggaca ataacctgac gtaattcata
    17401 acattacatt gactgataaa tataaaccaa tgaaaaagaa catttttaag ttttcggtac
    17461 tgacattggc tgttctgtct ttgacagcat gtacccttgt tcctggtcag aatttatcga
    17521 ccagtaacaa ggatgtcatt gaactacctg acaatcagta tgacctggac aaaatggtca
    17581 atatctatcc cgtcacgcca ggattgattg atcagttgcg cgccaaaccg attatgtcgc
    17641 aggctaaccc ggagctggag cagcaaatcg cgaactatga atatcgtatc ggcattggtg
    17701 atgtgttgat ggttacggtc tgggatcacc cggaactaac cacccccgca gggcaatacc
    17761 gtagcgccag tgataccggc aactgggtta acgccgatgg tgcgattttc tacccctata
    17821 ttggtcgcct gaaagtggcc gggaaaacac ttacccaggt acgcaacgag attacagcgc
    17881 gtctggattc agtcattgaa agcccgcaag tcgatgtcag cgtcgcggcg ttccgctcgc
    17941 aaaaagcgta cgtcaccggt gaagtcagca aatcgggtca gcagccgatt accaatattc
    18001 ctctgaccat catggatgcg attaacgccg cgggcggtct gaccgcagat gcagactggc
    18061 gcaatgtggt gctcacccag aatggcgtaa agaccaaagt gaacctctat gcgctgatgc
    18121 agcgtggcga cttgcgccag aacaaactgc tgcacccggg agatatttta tttattccgc
    18181 gcaacgatga tctgaaagtc ttcgtgatgg gtgaagtggg taaacagagc acgctgaaaa
    18241 tggaccgcag cggtatgacc ctggcggaag cgctgggtaa cgctgagggt atgaatcagg
    18301 atgtcgctga tgcgacgggg atttttgtta ttcgtgcaac gcaaaacaag cagaatggca
    18361 aaatcgccaa tatttatcag ctgaatgcga aggacgcatc ggcaatgata ctcggaacgg
    18421 aattccagct tgaaccttac gacattgttt atgtcactac cgcgcctctg gctcgttgga
    18481 accgtgtgat ttccctgctg gtaccgacga tttccggggt gcatgacctg acagagactt
    18541 cacggtggat tcaaacatgg cccaactaaa atttaactca atcctggtgg tttgtaccgg
    18601 caacatttgc cgttcgccca ttggcgagcg cttgctgcgt aaacgtctgc cgggcgtaaa
    18661 agtcaaatcg gcaggtgttc atggtctggt aaaacaccct gcagatgcga cagcggcaga
    18721 tgttgccgct aatcatggcg tatctcttga aggacatgcc ggacgtaagc tcactgctga
    18781 gatggcacga aattacgatc tgattctggc aatggagtcg gaacatattg ctcaggttac
    18841 cgcaatcgct cccgaggtcc gcgggaagac aatgctgttt gggcaatggc tggaacagaa
    18901 agagatcccg gatccctatc gtaaaagtca ggatgcattt gaacatgtct acggaatgtt
    18961 ggagcgcgcc agtcaggaat gggcgaagcg cctcagccgg taatttgagt ttataaaata
    19021 cgatgacaac taaaaatatg aatacgccac caggcagcac tcaggaaaat gagatcgatc
    19081 tgcttcgtct ggtcggcgag ttatgggatc accgtaagtt tattatcagc gtgaccgcgt
    19141 tattcacgct gatcgctgtc gcttactcgc tgttaagcac accaatttat caggcagata
    19201 ctctggtcca ggttgagcaa aaacagggca acgccattct cagcggcctg agtgatatga
    19261 tccctaactc atcgcccgag tctgcaccgg agatccaact gctgcaatcg cgcatgattc
    19321 tcggtaaaac cattgctgaa ctgaatctgc gcgacatggt tgagcagaag tattttccga
    19381 ttgtgggtcg cggctgggcg agattaacca aagagaaacc aggtgagctg gcgatcagct
    19441 ggatgcatat tccacaactg aatggtcagg atcagcaact gacactcacg gttggggaaa
    19501 acggccacta tacgctggaa ggtgaagagt tcaccgtcaa tggtatggtc ggccagcgtc
    19561 tggaaaaaga tggcgttgcg ctgactatcg cggatattaa ggccaaacca ggaacacagt
    19621 ttgtcctgag ccaacgtacc gaactggaag cgattaacgc gttgcaggaa acctttaccg
    19681 ttagcgaacg cagtaaagaa agcgggatgc tggaacttac catgactggt gatgatcccc
    19741 agttgattac tcgtattctg aacagcatcg ctaacaacta tttgcaacag aatatcgctc
    19801 gccaggcggc gcaggattca caaagccttg aattcttaca gcgccagttg ccagaagtgc
    19861 gcagcgagct ggaccaggcg gaagaaaaac tcaacgttta tcgccagcag cgcgattcgg
    19921 ttgaccttaa cctggaagcc aaagccgttc ttgagcagat tgtgaacgtt gataatcaac
    19981 tcaatgagct gaccttccgc gaggcagaga tctcccagct gtataagaaa gatcacccaa
    20041 cttatcgtgc gctgctggaa aaacgccaga cgctggagca agaacgcaaa cgcctgaata
    20101 agcgggtatc ggctatgcct tccacccaac aggaagtgtt gcgtttaagt cgtgacgtag
    20161 aagcgggccg tgcggtatat ctgcaattac ttaaccgcca gcaggagttg agtatttcga
    20221 aatccagtgc cattggtaac gtgcggatta tcgacccggc agtcactcag ccgcagccag
    20281 tgaaaccgaa aaaagcgttg aatgtggtgc ttggttttat tcttggcctg tttatttcgg
    20341 tgggtgccgt gctggcgcgt gcgatgttgc gtcgtggtgt agaagccccg gaacaactgg
    20401 aagagcacgg catcagcgtt tatgccacca tcccgatgtc cgagtggctg gataaacgta
    20461 cccgtctgcg taagaaaaat ttattttcta atcagcagcg ccatcgtact aaaaatatcc
    20521 ccttcctggc ggtggataac ccggcggatt ctgctgtgga agccgtacgt gcgctacgaa
    20581 ccagtctgca tttcgctatg atggagactg agaataacat tctgatgatc accggtgcga
    20641 cgccagacag tggtaaaacg tttgtcagtt caactctggc agcggtgatc gcccagtccg
    20701 atcaaaaagt gttatttatt gatgccgact tacgccgtgg ttattcgcat aacctgttta
    20761 ccgtgagtaa tgaacatggc ttgtcggaat atctggcagg taaagatgag ctcaacaaag
    20821 tgatccagca ttttggcaaa ggaggctttg atgtgattac tcgcggtcag gtgccaccta
    20881 acccatctga actgctgatg cgcgatcgga tgcgtcaatt actggaatgg gcgaacgacc
    20941 attacgatct ggtgattgtt gatacgccgc cgatgctggc ggtgagcgat gctgcggtcg
    21001 tggggcgctc tgtcggcacc agcctgctgg ttgcgcgttt tggcttgaac accgccaaag
    21061 aggtgagctt gtcaatgcag cgtctggaac aggctggcgt taatattaaa ggcgcaatcc
    21121 tcaatggtgt gattaaacgc gccagcaccg cttacagtta cggctataac tattacggtt
    21181 atagttactc cgagaaagag taaaactgaa ggtaaagtga ccggatgcgg cgtgaacgcc
    21241 ttttccggtc tacgcattag acagttcttc gtttgtcatc agcctcagag cattcaggta
    21301 gctgaatgct cttttttata cattacaaac tgcacgccgg tatgcgtgct tcattcacga
    21361 tttgcgtaaa accggccaac gaacacatgc cctgcgcatt tcgctcttca catcctgcca
    21421 gggtcagttt cacctctccg ggcggcgtat ttaatgacag cggagtttta tcacgcatct
    21481 gctgtaaagt ctggaagacc agcgaaacct gaatccactg gctgttatcg cttagccgac
    21541 gccagcgttc aaacaccagt tcaccacctg gcggcgtgtt atccggctga cctggaagcg
    21601 tccagttgag ctccagtgcg ccgccgagat ttgccagatt agtatcgtgt ccggcgataa
    21661 acagcactga agtgggtaat gtcacaccat acgcctgttt ttgcggtgga tggggagtca
    21721 acgctatcat gatcaaatcc aacaacgggg tggcgcggct gcgggcaacc tctggcgtgc
    21781 gttgtagtaa ataaaattgc gcgttatgca aacttagcaa cgtgttccac tggtgtgaat
    21841 cagtgatcct tccccacccc ggctccggca ttccctgtgc ctgttgcagg agaaatattt
    21901 ccgtcagcat tgatgcgagg cttaccgcac cggttaatga gacattgtcg gcgctcacct
    21961 tgagttccga tggtaatgcc tgcgttaatg aacagctttc gtcctgtttc tcacggttaa
    22021 ggcacaagtt tgattgcgga aaattaagca cccgttccag ttcgcgaaac gccgtttgcc
    22081 gatgcccggt aaagtcagca attgaccctc ctgccctgct gaggatcgcg tcagtcacgt
    22141 tcgcgttatc cagttggcaa acgccagttt ttagaggatt aaataacgga tcgggactgg
    22201 acgtatctgc ctgggtatgt acggttattg cacagtcagg tgccagcccg gcggcgaagg
    22261 cttcgcctgt tttacgggta cgctcgtcga catcagcaat aatcgcgacc tgaccaggct
    22321 gcgggcagcc ctttttcgtc agcaatccgt cggctaccag acgctggcgt tggtaatgtc
    22381 cgagataagc gattagctca ccaccgcgcg gtgtcagcca acccagtttt accggccagt
    22441 ttggccatgc gtctggggtg acatcctgca tcagttgcgt ggccttggtt ggggcacgca
    22501 caccatgacg actgacaatc accacacttt ccagcttcag ctccggctcc ggctcactct
    22561 gagcgaatgc agattgcggg gttaacggaa tcagaagaga taaaaatggg atcaagatcg
    22621 ctttcatcga tatgtttccg cttttcatat ccgacattat tgattgcctg atacgacgct
    22681 aatgcgccgc atcagactta cgttcgttaa cttttcaatc gcgggtccag ccataccaac
    22741 acaagggtgg agagcgaaca catcaacaaa atgccgacga accaaagtaa ataccacatt
    22801 gttcctgctc cttagtacag ctcgttttcg ttacggcgga gagtttctgt cgtcatgcgc
    22861 ccccacattt tgtagtagct ccagagagtg tagagcaaca caatgggcaa aaatatcagc
    22921 acgattacca acataatact cagcgtcagc tgactggaag tgctgtccca caacgtcagg
    22981 ctggagattg gactcacgct tgacggcatg acaaagggga acagcgtgat gcctgccgtg
    23041 aaaatcacgc caaattgcat caatgacgcc atcaaaaatc cccaacccgg gcggccacga
    23101 taaatcgcca tcaccgtcag caatgggcag aagaacccca gcagcgggaa gatccacaaa
    23161 acgggcgact cgacaaaatt attcatccag gcaccaggta gcactgccac cagtttcatt
    23221 aacggattgg aaggaccgtt agcatcctgg gcgagcagta caaagccatc aataccgacc
    23281 cacagccagt aacccgccag caaaaagcac agcatcacca acagtgcagc gcgtttagtc
    23341 gacagctgtg aacgcagatg aatcacacca acagttttca gttgtaacca gacgccacct
    23401 tgcaaaatca ccatcccaag gctgagcaat ccgcacaata aagggaatgg cgtcagcagt
    23461 tgccagaagc tgccgagata ctccacgcgt aattgcggag tgaaggcaaa cggcacgccg
    23521 agcaacaagt tgccgaaggc gataccgaag actaccggcg gcaccagact gccgatgacc
    23581 agaccggcat cccacatttt acgccagcgt gcatcggcga tttttccgcg ataatcaaag
    23641 gccagcgggc ggaagaacag cgagcacagc accaggatca tcgccacata aaagccggaa
    23701 aacgccgctg catacactct gggccaggcg gcaaataatg ccccacctgc gaggatcaac
    23761 cagacctgat tgccttccca gtgcgcacca acgctattaa tcactatccg gcgttcatca
    23821 tcatcacgcg ccaccagcgg cagcaaacag ccaatcccca tgtcaaatcc gtcggagatc
    23881 ataaagacca ccaggatcac gccaatcagc agccaccaga tgaagcgcaa tgtttcataa
    23941 tcaaacatga ttttctcctt tacccctgtt gctgcgtcgg ttgttcactc tgcatcgcgc
    24001 tcggccccag acgggcatat ttctgcatca ggtagacttc ggcgattaag aacagggtgt
    24061 aaagccctac gatcatgatc agtgagaaag ccagttgtcc tgtggttaaa gcggagtgcg
    24121 cggagtatgt cggtaagatg tcctgtatcg cccacggctg acgaccaaac tcggtcataa
    24181 accacccggc ttcaatcgca atccacggca acggcaaact ccagagcgcc attttcagca
    24241 cccagcgatg ctggtcgatt ttgccacgca gcgtctggac aagcgcaatc agcatcacca
    24301 gtagcagcag ggaaccacag cccaccatga tgcggaaact ccagaatacc ggcgcaacct
    24361 gaggtatcgc gccacgcatc gccgcctggt actgtgcggc tgtgacatga ttcatatccg
    24421 gcgcatagcg ggagagcaac atgccgtagc ccaggtcgcc ttccagctcc cggaatgcct
    24481 gcaacacatg cggctcacga ttgccttgcg atatttcctg cattaacagc caggccatac
    24541 gtccgcgttg caagcgtggg taggtttcag ccatcaaatt cttcagaccc ggcacgggtt
    24601 tatctaatga gtgagtggcg aggatcccta acagcacggg aattttgatg gcaaaggcgt
    24661 tacgctcttg atcctgttcc ggccaggcaa ccacatggaa cggtgcagga gcaggttccg
    24721 tttgccactc cccttccatc gccgccagtt ttaccggttg tacttgcgcg acttcatacg
    24781 cagaactgtc tccgagttgc agggtaccga taatcgccag agtaccgaag acggaaccga
    24841 tggcaaacga gcgtaatgcg acatcgcgct cccgtccgcg cagtaaatac caggcgctga
    24901 tcgccataat aaacatggcc ccggtcacgt agcccgccat tacggtgtgc acaaatttca
    24961 cctggctgac cgggttaaag accagctcgc tgaagctggt catctccata cgcagggtgt
    25021 cgatatcaaa atgcgcaccg gtcgggtatt gcatccaacc gttggcattc aatatccaca
    25081 atgcagagag atttgaaccg aacgccacca gccacgtcac cagcaggtgc tggtatttat
    25141 tcagccgctg ccagccgaag aagaaaagcc cgacaaaggt ggattcgagg aagaaggcca
    25201 ttaatgcttc catcgccagc ggtgcgccaa aaatatcgcc cacatagttg gaatagaatg
    25261 accagttagt accaaactga aactccatgg tcaggccggt agccacgcca agagcaaagt
    25321 tgataccgaa gagcttaccc cagaagcgcg tcatatcgcg gtagattgtt ttgccggtga
    25381 ccacgtaaat ggtttccatg atagccagca aaaaaatcag ccccagggta aggggtacaa
    25441 ataaaaagtg atacagcgcg gtcagagcaa actgccagcg cgataaatca atgacatccc
    25501 acatgcgcac tcctgtaggc gaagagagaa aaataatccg ttaatggtag atctccctct
    25561 ttatcctgaa actctacgac cagaggtatt cgccgattgc gatattcata agcctgaaag
    25621 cgatacttac cgtcttttta cgtcggtgca gcttccgcca gccactgaca tacctcacta
    25681 agccgctgcg cagagtcgac caaatcttct ggcgctgcca gcaccacttc cggtattggg
    25741 cagatttcat aactttccag taacgggcct tttaaggtgt ccgtacagcg taaatgccag
    25801 acatggcgta accccgtggc gttgatatag ctctcgccat agccaatggt acgaatctga
    25861 atatttcccg gcccacagag acgtgagaga aaaagccgat cagcctcgct gatgggtaat
    25921 tgcgtcagat taatgctgtg cggctgcgcg tcggggttac gtacatgcgc cagtaactca
    25981 tgcgccaacg gtaggccatt catcaggcca tcaatgggcg gcggtaacag cgaatctgtc
    26041 ggcaagagat tttgcgttgc cgcctgccac aacgccagcg gcgcgcagcc agcctccagt
    26101 ttgtcctcca gcaacttttc gccgcgacgt ctgcgcaccc gccataatcc gcagaagatc
    26161 gcctcctgta tttcactttc gctgtcgtca gcctgctgaa tgcgcactga cacttcacct
    26221 tccccgagaa gcgtattgat aaagtgacga tcgtcggcgt tgagatttaa cagatcgact
    26281 gccagcacct cgcccaacgg cggctggcgt tgacttagtt gatgttgtaa ctcgtttaac
    26341 agcgcaatca cctgcgggct gtgagcacat tgctccaggg ccgccatact cggttcatca
    26401 ttcacctgac aggtgatcgg cagtggattc atgctgaaac tgtcatcgtt cggttgcgtt
    26461 cttggcccca gcagatggaa aaaagtttcg ctcatgaggc acgctcctgc tgtggttcga
    26521 caagcccacg catcaggttt atcagttccg cccacgggtg aataccattc agcacgccgc
    26581 gatagtttcc gccggtaaac actaatgtgg cgggaaagcg aaagacgcca aagcgatctc
    26641 cgatggcttc gctctgttca aggtcagcaa tcgccacctg ccatgtgtag tcgggaaact
    26701 cgcgcagtaa ttcgccaatc attaccggat tatcgctgac ctctggcgtg cgtttcgggt
    26761 cactgcttaa taacaccacg ccgtctggcg cttgcgtaag ccagtcgtca agacggaatt
    26821 cactaaccgg cgtccagccg cgcgccagca ttcgttgcca caacgcatca aatggcgtgt
    26881 cgttgctcat ccctgctcct ccagtgtcat cctgtactgg cgcaagcgaa cgccttcgta
    26941 attttccatc gacagacagt cataattgag acagcgcgat tcattagccg gttgcggcac
    27001 aattccccat gcagccagct gcgccagcgc cgcctgttcc gctgcgggca gttgctcccg
    27061 tgccagttcg ctcaggctgc cgccgtagtc gtcgagcatt gcgggttgca gaccgacgag
    27121 ggcaatatgt gctggcagat gtccgcggat atctgccagc gccagcactt cggagaaact
    27181 gttctggtgc aggctcattt tcttcgcgct gagataagcc ggaatacgtt ctccggcata
    27241 ggttcgcagc gttcctggtt ccagcccgta gtcaatggca tcgagaatca acagatggct
    27301 ggcgctttcg acatacccca gcaagttcag tccctgagta ccgccatcga caatctccac
    27361 agactcgggc cagtggtaat gggcatacaa ccgttccgcc acccgcacgc cgaaaccttc
    27421 atcggcccac agcaggttgc ccagccccat taccaccacg cgttgctcgc tcatgaacgc
    27481 tccttgttac ttattttgcc aaatttgtgg ctacggtagc cgttgaccat ggtggagatc
    27541 accgtgtcgt cggacatgat gtcttcacgc agcgccatat agacatgacc gatcacaaac
    27601 gcgccaatca gccacatccc cagccgatgc cagctgtgaa tgtccattga gttgcctccc
    27661 gtccagtaga aaaattccac cacataacgg aacggcgcaa aaatagcgta ctggctgtgt
    27721 tcgctgtaca gcgcaaagcc ggtgatgatc ataaagaccg acatcaggaa atagccaaac
    27781 atcgccgcct gggcgatggg gttatggcct atatcggcac tcggacgttt tgccagaaac
    27841 agataccagc ggatttcata ccacacgccc tgccaccagc ttttacgcca tactggcacg
    27901 ataaacagct cgcgggagta tcgattgcca acaaaagccc agtagatccg catcagcaag
    27961 accacggtaa aaaccatccc ggcgctgaag tgaattaacc tgatgtagcc catatagaac
    28021 agatacgtcg cctcgccgct gacggaaggt agcggtttgc cgataaagta accggtgacc
    28081 atcaacaccg ccatgcataa caccgtcagc cagtgccaga tacgcactgg cgcttcaaag
    28141 acatagtggc tgacaacgtt gtcgcttttc tgttgcatga tgattctcct tcgctgttaa
    28201 cgcacctgca cggagatcag ctcgctaccg tcgtcgccca gcacgtgtgt tgaacaggcg
    28261 aggcacggat caaagctgtg cagagtacgc aggatctcca gcggttgctc ggggatcgcc
    28321 attttggtgt tcatcagcgc cgcttcataa gcgccaatct gccctttggg atcgcgcggg
    28381 ctggcgttcc aggtggtcgg caccacgcac tggtagagat caatcttgcc atcgcgaatg
    28441 gcggcccagt ggcctaacgc cccgcgcggc gcttcggtaa aaccgacacc acggcactct
    28501 gtcggccagg ttgtgggttc ccatttttcc gtggaggcgg tggcaagatt gccgtttttc
    28561 aggttagtca tcagcttgtt gaagaaatac tgcaacttac ctgcggccca ctgtgcttcg
    28621 tgcgcgcggc acaaaatgcg gcccaacgtt gactggatac cggaaagcgg caggttcagc
    28681 gccgacatca tgcgatcgac cgactcaacg gtcgcagcat cgcctttgtg ataagcgatt
    28741 aacgtgcgtg ccagcggccc cacttccatc gcgttaccgc gccagcgcgg cgctttgatc
    28801 cacgagtagc gttcctgttc attcagctgc tgaatgttgg tatcgctgcc tttgacatcg
    28861 ccggggttgt accacgggtc ggtgatgcca tcgaacggat gacgcccgac ctgatcgttg
    28921 ggataacgat accaggcgtg atcgacaaac tcctgcacct gctgcggatc aaccaaatcc
    28981 actggcagca cattgttgaa gtcgccgtta atcaccgcgc cgccaggcat cagcagactt
    29041 ttctcaccaa agtcgttggc aatatccggg aatgcgccgt agctgaggac acatttatca
    29101 gaaagacccg tgccgatttc gctccacggt ttgttgaact gaccgatggc taaggcgtcg
    29161 gggatcatca cgttgttaat gaagtctgcc gtgcgggtga taatcgactg caccaggttc
    29221 aggcgttcca tattgaccgc cccgaccgcg ccgctttcgt caatgttaat ggcgcaaggc
    29281 atcccgccga caatccagtt tggatgcggg tttttaccgc caaagaccgc gtggattttg
    29341 acaatttcac gctggaaatc gagagcttcg agatagtggg caaagcccat caggttggct
    29401 tctggcggca acttgtactg cgggtgcccc cagtagccat tgcggaagat ccccaactgc
    29461 ccgccttcaa cgaatttctt caggcggttt tgtacgtcga agaaatagcc aggggatgat
    29521 ttcggccaag aggagagact ttgcgccagt tcggaggttt tccgcgggtc ggctttcagc
    29581 gcatccaaca catcgatcca gtccatcccg gcaagctgat agaagtgcac cagatgatcg
    29641 tggcaccaga gcgttgccag cataatgttg cggatgatat tggcgttgtc cggcacttta
    29701 ataccgatgg catcttcgat ggcgtaaacc gaagccagag cgtgtacgcc agtacagacg
    29761 ccgcagatac gttcaacgaa cgcccacgca tcgcgcgggt cgcgcccttg caggatgatc
    29821 tccagcccgc gaaacatggt gccgcaggag acggcattgg tgattacatt ctgatcgtta
    29881 atattcactt cgcagcgcat gtggccttca atgcgcgtaa tcgggtcgac caccaggcgg
    29941 cgtccggcat tattgatggt gtatccctga gtttcgtact gagtgctcat gcctgtttat
    30001 cctcattgcc tggctgatgt tcggtttctg taggttgctg attatgacgt ctgcgctggt
    30061 caacggcgct ggcgactgcg tgcacaccaa cagccgctgc caccacacca agcgcggtta
    30121 aaccgacggt atcggcggtg gaatgagtac ccatttgtgg aatatcgacc acgcggctgt
    30181 agaacgaacc gcgatcccag aaaccatttt ccgcacagcc caggcagccg tgaccagact
    30241 ggattgggaa agaaacgcca tcattccagc gtgtggagga acaggcgtta taggtggtag
    30301 gccctttgca gcccattttg tacaggcaat aacctttgcg ggcagcgtca tcatcccaac
    30361 tctggacgaa ctctccggcg tcgaagtgag cgcggcgata gcatttatcg tggatacgct
    30421 gaccataaaa catcagtgga cgacccattc tgtcgacatc tggcaagcga tcaaaggtca
    30481 ccatgtaagt aatgatggcg ctcatcacat ccgggatcgg cgggcagcca ggtactttga
    30541 taatgggttt gtcggtgatg actttgtcga tagacgttgc ctgtgtcgga ttaggtcgcg
    30601 cggcctgcac gcagccccag gacgcacagg ttccccaggc gataatcgcg ctggctccgg
    30661 cagcggcacg tttgagtttc tcaataaacg gtcgaccgct gctgatacag aacatcccct
    30721 gctcgcccag cggcggatta ccttctactg cgaggatata tttgccattg tattgcgtga
    30781 tgatgtcttc aaagacttct tccgcctggg ttccggcggc agccatcaaa gtatcgtcgt
    30841 aatcgaggga aatcagggaa aggatgacgt cctttgccag cgggtgagcg gagcggataa
    30901 aagattcggt acagcaggtg cattccagac cgtggatcca taccaccgga atgcgcggtt
    30961 tgttctccag cgcccaggca atctttggtg ccattcccgc gcctaatccc agcgacgtgg
    31021 cagccagact acaatatttg agaaagctgc gccgggtaac gccctgacgc cgcatggcct
    31081 ggtaaaatgt ttcctcgtta ttcatatcgc acgtctcttc tccttgcgac accggcagga
    31141 caccgggcgc ttatggtcgg caagcgacgt ccctgtagca aggcaaacca tcgcttcacg
    31201 tgagatgctg aaaacgaaag ctcatccttc tgcactggcg cacgtcgcca gaaagtattg
    31261 ttaataaagc gtagtgaaac ttttgcacaa aacaatacaa actgtgtgga tttatctttt
    31321 agcgataaaa atggacctat ttttcttttt gccgggcggt gggatgttta gccagttgct
    31381 aaacgagtaa aagagaagga atttatggtg atgaaatgat caatctgctt ttatttcacg
    31441 ctatcaatgc acttttggct gtttttcagg caaacaaaca gattaggggt ttacacgccg
    31501 catcgggatg tttatagtgc gcgtcattcc ggaagtgtgg ccgagcggtt gaaggcaccg
    31561 gtcttgaaaa ccggcgaccc gaaagggttc cagagttcga atctctgcgc ttccgccaaa
    31621 taatcaaggg gttaccaaat ggtagcccct ttgctttttt ggctcttggt ataaacttgg
    31681 tataccaatt ggtatatttt tattcggatg agattatcgg tcatgagcgg aacctcaggt
    31741 tcctcgtctg atgccgctct tgcaacacgt tacgctgccg agtatttttg taaaacatgg
    31801 actgctccag gcctgaacca ggcagaagga tacaaagcca tctcagatct gtcgcatcac
    31861 tattttcgtg cagaaggctc tagccctccc caatcttttt tgtgacaccc tgtttcactg
    31921 aacatgaaga cactcacctt gtccctgata ttcattagca tcatcctgat tatttaactt
    31981 ctctatttag ggaagcccgt ctgatacaaa gcttctattc ctcgttaaat attctccatt
    32041 ctggcgccaa gccctatcta tagctgattg aaaacaactc taaaaacgta ataatatcaa
    32101 atagtttatt cctacagccg atcattttga gtaaaatatt gacgagaatt tcacaaaagg
    32161 tagatttc
//
LOCUS       DAZFTN010000055        31340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFTN010000055 DAZFTN010000000
VERSION     DAZFTN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..303
                     /locus_tag="ACHKV2_002682"
     CDS             <1..303
                     /locus_tag="ACHKV2_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940545.1"
                     /translation="KMKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDN
                     NKIRDYKTGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLR"
     gene            313..729
                     /locus_tag="ACHKV2_002683"
     CDS             313..729
                     /locus_tag="ACHKV2_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6940546.1"
                     /translation="MQGHPCIFLKGVDFMKSVYVVGKGLIVAGNKITIDGKEHVSVEN
                     SENIVVTDDCIKIGNKTVVFKGRSIKVEIHGNVNKIVTENADVSIHGNAGDVETVSGD
                     VDCHQVNGDITTVSGDVTCGDVSGDVTTVSGDINRK"
     gene            744..1037
                     /locus_tag="ACHKV2_002684"
     CDS             744..1037
                     /locus_tag="ACHKV2_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940547.1"
                     /translation="MLTEAKKMAVEMMFWPACPVDDDYCFDAIMYTSVIDRKFEDEHG
                     VYLHQVYKELCSTPELKKFLKKKAIKLIAFKHDIDLDSANKILMKNMLRRIEN"
     gene            1106..1255
                     /locus_tag="ACHKV2_002685"
     CDS             1106..1255
                     /locus_tag="ACHKV2_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940548.1"
                     /translation="MSERILSAIHDVEKGGRPVFPLMPFHVFPEYMALLRKALEKKTQ
                     KRTDK"
     gene            1268..1540
                     /locus_tag="ACHKV2_002686"
     CDS             1268..1540
                     /locus_tag="ACHKV2_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940549.1"
                     /translation="MTKHEFVSFVSGELRQGAVRFSLAFNSKGEIVLYWTNKAGIRVW
                     RILSGNRGKKPSKANLERMSNFRRWLFDARQGMEGYTQQPEQSNLS"
     gene            1856..2401
                     /gene="ddp1"
                     /locus_tag="ACHKV2_002687"
     CDS             1856..2401
                     /gene="ddp1"
                     /locus_tag="ACHKV2_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HFO6940550.1"
                     /translation="MKQVNFRLEEQQHDELLDCLKILYPDEPALTVAKGMKLLASALL
                     KSKAGSKDINTFFDNKDFIKTTMYLTGKQRADIERAANRHGWTLSRECRYRIQTTLEN
                     ELDFFDQELLMMNRCRNSIDKIGRNFHYIIVNDQTRVLDKDGFYQDAERLTTEIFNLK
                     NQFENYIMLCKGRTVSNKVEM"
     gene            2404..3570
                     /gene="mobP1"
                     /locus_tag="ACHKV2_002688"
     CDS             2404..3570
                     /gene="mobP1"
                     /locus_tag="ACHKV2_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP1 family relaxase"
                     /protein_id="HFO6940551.1"
                     /translation="MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERIN
                     RKGASKEVVVKISGGAITRQGVRNSIDYMSRESELPVMSESGQVWTGDEIQEAKEHMI
                     DRANDPQHVINDKGKENKKITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVL
                     GYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLRGYDVKATHKQQ
                     HGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQYFIKLKTLNKGVEKTYW
                     GADFGDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGV
                     KGIDRTPSASKELVLNSPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL"
     gene            3903..4409
                     /locus_tag="ACHKV2_002689"
     CDS             3903..4409
                     /locus_tag="ACHKV2_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO6940552.1"
                     /translation="MKWYVLQFTTTRFAAVFAHLERLSFSYFCPMETERYRRPDKIVS
                     YRERCFPLFPGYLFVQADFEEVHSTIITAIPYVQRFISFGGEPLPVSEDVIEVLLRRQ
                     SQSAQTNLLRKSIPHEFAEILLMENPQQRSMAFIHYITERSLTHKMKRKKNDCYPEKK
                     RNETQAPT"
     gene            4633..5280
                     /locus_tag="ACHKV2_002690"
     CDS             4633..5280
                     /locus_tag="ACHKV2_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6940553.1"
                     /translation="MLSTSTFLALAMQCAASVHPDTTHEVARVESGFNPYAIAEIIPK
                     VKRKPGDKGVVSYFPESKEAALSIVKNIESRNHRYSVGLMQITSTNFAKFGTTAEKMF
                     DPCENLKVSEKILVDCYKRGGDLVRGLSCYYSGNPETGVKPEPEFNNTSYVQRIGFSP
                     PDNKKIFIVPSVKEMIKKENKTTITPEEIIIYPQYAIRGTVSNEKETKDVEIKSE"
     gene            5258..5554
                     /locus_tag="ACHKV2_002691"
     CDS             5258..5554
                     /locus_tag="ACHKV2_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFO6940554.1"
                     /translation="MSKLNLNKRYLTLSLFMAALMLCVAEPAFADDVSTKTTGFLQKI
                     IDFLTNVRKPAITIMALVIGYIALFSRQHTAWIVPLVIGIIIFIVAPYIPNWLA"
     gene            5573..8326
                     /locus_tag="ACHKV2_002692"
     CDS             5573..8326
                     /locus_tag="ACHKV2_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO6940555.1"
                     /translation="MSTLFKGLTRPALIRGLGVPLYPFLGMCIICVLLGVWIHEAMYA
                     LILPGWYAIRRVTQFDERFFDLLYLRTLVKGHPLSNKRFSAVHYAGSQYDEVDISKVD
                     NFMKLKDQSSVEELIPYSSHITDNIIVTKNRDLLATWQIDGAYFECVDSEDLSILTDQ
                     LNTLIRSFEGKSVTLYPHRIRCKKDVRPVFNSKIPFVNRVMNDYYESFPQSEFFENKL
                     FLTICFKPFTTEDKVTHFFSRGKKQKDIFKEPVNEMNEICDRLNTYLSRFHSRRLGLY
                     EDHGVVYSDQLSLFQYLLSGRWQKVRVSSSPFYTYLGGKDLFFGNDAGQITASDHARY
                     FRCIEIKDYFQETDAGILDALMYLPVEYVVTSSFTAMDKQSAIKALDDQIDKLEMTDD
                     AAKSLLADLKVGLDMVSSGYISFGKSHQTLVVFADSPERLVKDTNIVTSTLEDLGLIV
                     TYSTLSLGAAYFAQLPGNYTLRPRLSTLSSLNFAEMESFHNFFSGKEKGNTWGEKLIT
                     LRGSGNDIYHLNYHMTTEHQNFFGKNPTLGHTEILGTSNVGKTVLLMTKAFAAQQFGT
                     PESFPANRKLKKLTTVFFDKDRAGEVGIRAMGGSYYRVKEGEPTGWNPAALPPTKRNI
                     AFMKDLVRLLCTLNSEPLDDYQNSLISDAVERLMQRSDRSYPISKLRPLIQEPDDTET
                     KRHGLKARLKPWTQGEEFGWVFDNREDTFDVDNLDVFGIDGTEFLDNKVLASAASFYL
                     IYRVTMLADGRRLLIYMDEFWQWINNEAFRDFVYNKLKTGRKLDMVLVVATQSPDELI
                     KSPIAAAVREQCATHIYLANPKAKRSEYVDGLQVRELYFDKIKAIDPLSRQFLVVKNP
                     QRKGESDDFAAFARLELGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTYLEQA
                     NLI"
     gene            8337..9095
                     /locus_tag="ACHKV2_002693"
     CDS             8337..9095
                     /locus_tag="ACHKV2_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO6940556.1"
                     /translation="MKKQIMAAFVASLIVISGAQAGIPVAIDANPEWAIEAGRWTERL
                     KQWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDY
                     IKNPEGALSEQAKSLLSDYKVTDTCQNLGYSGDLVRGCEATFLSQLASVEYGNKLESK
                     LRQDNQTMKDLIDQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYRDKQRDLAEY
                     KEKMAQASFRKQQREAVPPSYRKAYMAMKSYEDD"
     gene            9096..9323
                     /locus_tag="ACHKV2_002694"
     CDS             9096..9323
                     /locus_tag="ACHKV2_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO6940557.1"
                     /translation="MKSIATAGLVIISVFSLSGCFEETKSVDWWLAHPKETYKKFEEC
                     QKSGSDSDNCKNVKRAHLSFERRKAVGLPIN"
     gene            9333..10466
                     /locus_tag="ACHKV2_002695"
     CDS             9333..10466
                     /locus_tag="ACHKV2_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO6940558.1"
                     /translation="MGIVTGIPDGTDLSGNVTSPANAGGVPGFDSDFFQTTHEVIFNI
                     LNKSISGKLSEYSDVAYTLGKYGVSLYVLWYAFTVLARKQQTPVPDFIWNICRFYIIL
                     LFVKNTGGILTSATDAIDGLKNTLAGGDPWVWMDQLWVKVIQVATLIFDKDTSTVPVA
                     GGIGALLTYVGGVLALLLCSIVFAAAELTLLLLSVTAPIFIMCLMFGFLRQMFNSWLQ
                     LNFSSLLVFLFAALALRAGTWQLNMALSTSIATASENNLLQTGVTSLAAGIFMAWIIW
                     QAKSYASQIAGVGVEGAMQGAAAMGIGAGIFGASRMARGALGMGRNAGIGAWKGLRRQ
                     EGGFGQSPGITGKTANLAGQGVNIGAKKLRQAAIERAKKMYGG"
     gene            10711..11424
                     /locus_tag="ACHKV2_002696"
     CDS             10711..11424
                     /locus_tag="ACHKV2_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFO6940559.1"
                     /translation="MEFKLPGFKNKKDVTDSSVSFEEKNIALQERMNRIYKFGGIGGM
                     LIGGLSLLALNAALPLKTTVVDAYLIDKVTGVAERLTSVKKENLSENEAIARYFITQY
                     IKHREGYNFFSLQHDYDYVMAYSAENVAADYNALFNSEQAPKLVYNKAEKTAMVQDNP
                     SVIISPSSRTDDKDIGAYIRFRLTIRDVATGQTRQEFWNVRLTYRIEPQVEMVSGERN
                     NNPLKFVVTSYVRDKEARG"
     gene            11429..12358
                     /locus_tag="ACHKV2_002697"
     CDS             11429..12358
                     /locus_tag="ACHKV2_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO6940560.1"
                     /translation="MKMNKGALIMALLMAAHVCNAAVLPSGSRFDPRNQIVSYNPNNT
                     TIINSAVGYTTTLVFDEDETVISARTGFPQGWAVNKEDNLVYLEVRPVKQTVQKNNTD
                     ENGNTSSESVSVALDPENELERWRTNLFVRTTKRNYSMELNARTFRQPEKIAFVVNYQ
                     YPQERRKEQAEIEKKRTETLARRLEEQAINRSLENAKSPRNWQYWKRVAEGSQDISPD
                     YAYDDGRYTWFGFSPLKKIPSVFVMNGMQETLTNPVIKQSGSFTAVGVPVDKRFVLRL
                     GEQVVGIENQGFGKVRLPAGDTVSPDVKKEVIQ"
     gene            12355..13560
                     /gene="virB10"
                     /locus_tag="ACHKV2_002698"
     CDS             12355..13560
                     /gene="virB10"
                     /locus_tag="ACHKV2_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="HFO6940561.1"
                     /translation="MTEQENKIPTATEIEQQLRERRQKELEQAGKTPEEEPGKPALQL
                     GIEKLKKSRKGMIILVVGFLLLAAGVSVYYIPSIIRSVSSGDEKPASQPLATGEAKRQ
                     TGLSEDIDPFNTAQKKTEKPEEEKVISSEKTEPPENKQQSFSRALDVSLDGSQAGNSS
                     SSAGTSVSHTAASEPESDKKDEAKATAQATESAPLAKITKLPYDSNLFIPEGTSIPCS
                     LDRRFVSDLAGKLECTVNSDIYSASGNVKLIERGTAAKLMYKAGSLNHGQGRVFVMAY
                     KLRTRSKPFIDIPLVDSQAAGALGEAGASGWIDTHFSERFLGAMMVGMIPDLSQAASG
                     IAQNNRDSQTDYTANSRQAFADIAREAFSNSVNIPPTLYKNQGEIITLIVGQDLDFSG
                     IYKLKMKGG"
     gene            13562..14593
                     /gene="virB11"
                     /locus_tag="ACHKV2_002699"
     CDS             13562..14593
                     /gene="virB11"
                     /locus_tag="ACHKV2_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO6940562.1"
                     /translation="MNNENRHLIYDVVNDYFYHWLNETDGVTEIAVNRPGEIFIKVRG
                     KWQWYEQKMSYSDCLSFASTLADFHDGGSVTPEYPLRSATLPGGERVQVVIPPATEKD
                     TVSITIRKPSGIFISHDKFIKQGFYSRVSGLSGDSVMEDNISALITSGYFERVIPESL
                     SQGKTIVFCGGTGSGKTTFANACLEYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPAE
                     GESKIITSASLLRSCFRMNPDRILMTEIRGAEAWDFLKASSSGHAGNITTVHESSPEY
                     AVLGIVQRCYMNPECQNLPFNVILRRVLSNIDIIMSIKYLDDEDFRFASGIYYRQLHF
                     DDYFRKLKE"
     gene            14596..16431
                     /locus_tag="ACHKV2_002700"
     CDS             14596..16431
                     /locus_tag="ACHKV2_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO6940563.1"
                     /translation="MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKN
                     FDSMLLWKIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFVIWQLNKMDVALYGDAKF
                     ASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPN
                     LLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAES
                     GAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKP
                     VFSIGSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQR
                     SSIDGSFRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNL
                     FFNFVVEVTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIY
                     QNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKISEKLGYITTTSKSTSKSRG
                     RSASQGESESEARRALVLPQELGALDFKEEFIILKGENPVKAEKALYYLDPYFMDRLM
                     KVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL"
     gene            16428..16841
                     /locus_tag="ACHKV2_002701"
     CDS             16428..16841
                     /locus_tag="ACHKV2_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HFO6940564.1"
                     /translation="MKKILMISILVLTACSSPPEPPQVEWEKMPEVMNTQIMNWTPTS
                     NVIKSDNINSLWSKVLPDFKPENRLYDDSVFYAVAHSEKIVVRTSSFDSYWSAKDWLR
                     KNGATGVIEYQPLKRWLNNDYVEIYLSRINVQRLP"
     gene            16838..17140
                     /locus_tag="ACHKV2_002702"
     CDS             16838..17140
                     /locus_tag="ACHKV2_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO6940565.1"
                     /translation="MKKICKGLIVIFTVSCFIAPTYAADPCKSVFCLYGKAVGSSGGS
                     ECSSAEKDFFKNVEKKKGKIRWGKTFDLRKNFLNQCSTADPAAISLIMSKFGKIKG"
     gene            17146..17403
                     /locus_tag="ACHKV2_002703"
     CDS             17146..17403
                     /locus_tag="ACHKV2_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940566.1"
                     /translation="MKKKEKIDFLNAIKAGHSGFIAGNPAHALDDKTMNAIIHNYEAT
                     IAPGKAGNCKETPLTKYCSRTMPERISVEEINEVKIYPELF"
     gene            17485..17919
                     /locus_tag="ACHKV2_002704"
     CDS             17485..17919
                     /locus_tag="ACHKV2_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940567.1"
                     /translation="MMNKFSYVNRMLRQVFVFSFITAPSIALFVVFMFFSFNNSIAGS
                     FLKEARGLITDTPSDKVNVCLFEKGVPQDMELTNSTTEHVLKNKCITTLVDAADWQES
                     VDGSIRYFYWSSVVIGFVVWLTFNFRALEELFYRRKRKGDVE"
     gene            17916..18431
                     /locus_tag="ACHKV2_002705"
     CDS             17916..18431
                     /locus_tag="ACHKV2_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6940568.1"
                     /translation="MRIFLSVILFLFTAFQSANAGGIPGALAGILSTLNQEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGHVFTTNGTDASRFMVQSGAAWVYERYNVDESLPALQREAQEQKRGLWVDANP
                     VPPWEWRYKHN"
     gene            18447..18851
                     /locus_tag="ACHKV2_002706"
     CDS             18447..18851
                     /locus_tag="ACHKV2_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6940569.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPEYPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            18911..19231
                     /locus_tag="ACHKV2_002707"
     CDS             18911..19231
                     /locus_tag="ACHKV2_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940570.1"
                     /translation="MNIHLFSEVLFCVWVIALIVILFIVVKYYRRVHYRLNSLSETIK
                     RTQGGVNKRISENRELLELIKNQHPEILDEYPWVSGWLDSQEKFLVALADKSGIDINK
                     SGLI"
     gene            19258..19845
                     /locus_tag="ACHKV2_002708"
     CDS             19258..19845
                     /locus_tag="ACHKV2_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFO6940571.1"
                     /translation="MYIHIVPKLYHLMANKCSLKSISIPELDFIIDSESLSVGRPWPN
                     KCLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHQVNTYIEDDNFDMV
                     SQEILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLL
                     SREESLLLHTIQSERLSTDCSLFKRQPSIESALVI"
     gene            19857..22010
                     /locus_tag="ACHKV2_002709"
     CDS             19857..22010
                     /locus_tag="ACHKV2_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO6940572.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHI
                     IESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDSSRKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     TIKKLLKDKDKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDA
                     LPGIAVNPDMTALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAP
                     AGQFQRLDSPCPSCGKHIVIRPKGYFCTGCEFKIWSEFYGKKITQAQAEKLVKSGKTD
                     LIKGFKKKSGGTYDTVLVLEDKKTGKLGFPARAKK"
     gene            22007..22213
                     /gene="hha"
                     /locus_tag="ACHKV2_002710"
     CDS             22007..22213
                     /gene="hha"
                     /locus_tag="ACHKV2_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6940573.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAAD
                     HRRAELVMNKLYDKVPSGVWKYVH"
     gene            22229..22693
                     /locus_tag="ACHKV2_002711"
     CDS             22229..22693
                     /locus_tag="ACHKV2_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO6940574.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREVDFEQLLEMQEKLNV
                     VVEERREEAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGG
                     VRKAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIDKLQPGQVE"
     gene            22797..23453
                     /locus_tag="ACHKV2_002712"
     CDS             22797..23453
                     /locus_tag="ACHKV2_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940575.1"
                     /translation="MMIKSKKVVVIFVALSSFLLAGCDSPKDANKQNFEKAINTRLEK
                     ECITISPLGMFDSKPYPVQVAVAQANRYTSQERADEINSRQFSALDVLVKADLLTVKD
                     TLVDDVIGFTKTGKKVPGREYALTDEGKKYLKSPERPDFCVGHYKVDEIVDFTEPGDA
                     MGMKITQVNYTFSPTSIAEWAKRDDVRAAFLGLESDLKEKQTKRITLVLKNDGWSAER
                     "
     gene            24009..24824
                     /locus_tag="ACHKV2_002713"
     CDS             24009..24824
                     /locus_tag="ACHKV2_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6940576.1"
                     /translation="MSHLPASIFQISRSDAWNLTLSPDAARNMRDLAAQIGAGMPKYL
                     IAPEVSLLLSYIDDLSIRMYFETLWNTGARLNEALALKPADFMLTPTRQYPQPVVILK
                     TLKQRIKEAERRPGRPTTRHPPLHPDPRYRKPPEPAVRLVPVLDAGYALRMREYLATW
                     KKRIKHQPVWDITSRQTPLNWINTAVARAGRDGVTFSVPVTPHTFRHSYAMHLTMSGV
                     PPRVLQSLLGHRYARSTEIYTRVFSLDVLTGSGLSFTCDPQLAKQLLGYPDAP"
     gene            24939..25598
                     /locus_tag="ACHKV2_002714"
     CDS             24939..25598
                     /locus_tag="ACHKV2_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HFO6940577.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIIKGKTACFLETDSQGS
                     IKDFIEERKTNDRLSEIPYFECYTDIPAMARKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADILFTFIEPGAGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASEL
                     RRQLNDDPDWLPVPRQRIYMRTVHKKAYNSGMGVHEYDDKRGNKARGEIELLLKETDV
                     L"
     gene            25692..25994
                     /locus_tag="ACHKV2_002715"
     CDS             25692..25994
                     /locus_tag="ACHKV2_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940578.1"
                     /translation="MAKLPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDD
                     DLDTAQVFQNKYGASRAEVIRAALAALRLVSAEERRRLFDDIRKNSPKAGRPTVNK"
     gene            26031..26315
                     /locus_tag="ACHKV2_002716"
     CDS             26031..26315
                     /locus_tag="ACHKV2_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940579.1"
                     /translation="MLIKIIKSVFNFYMLCVQPLLGLFLMGYSIWSFSGDGICTADKV
                     IACDISIYLEPAFYLLIGTVVLFVWYIAWQTERSKEEDFKSSIKPEDFVK"
     gene            26477..26758
                     /locus_tag="ACHKV2_002717"
     CDS             26477..26758
                     /locus_tag="ACHKV2_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940580.1"
                     /translation="MSEIYCIAIAVIIVGYVYLMISVITAPQKLFPILQLITLIGISS
                     LFSFMSFKSESMWTGEPELLVAIMFGTFSATLIFYMFKILIRAFDSLTK"
     gene            26909..27124
                     /locus_tag="ACHKV2_002718"
     CDS             26909..27124
                     /locus_tag="ACHKV2_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940581.1"
                     /translation="MEHCIVDLGIINYHPVITWTVPVAFFFLVVLAGIAFNIGRKSNL
                     IVFILAMLLPVTTVFIFMLLGGKIDMC"
     gene            complement(27507..27860)
                     /gene="ddp1"
                     /locus_tag="ACHKV2_002719"
     CDS             complement(27507..27860)
                     /gene="ddp1"
                     /locus_tag="ACHKV2_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="HFO6940582.1"
                     /translation="MKYQVKEFVNEKYSKAINILKDNLKENYHVFYGVRLSEILFPAS
                     EYGTDAFFKEFESINSITLPLIIFEMNERKPVAIISFEQVAGSVFVGQFDINVLVVEN
                     LSELLTNETLDSLYN"
     gene            complement(27990..28433)
                     /locus_tag="ACHKV2_002720"
     CDS             complement(27990..28433)
                     /locus_tag="ACHKV2_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940583.1"
                     /translation="MAKIYQFPLGEERGKFRKEIARERKKRFAVKTGSIFVKWLGWGW
                     FYLRLLAASVLHFVSVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPV
                     ITATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSSEISQ"
     gene            complement(28442..29434)
                     /locus_tag="ACHKV2_002721"
     CDS             complement(28442..29434)
                     /locus_tag="ACHKV2_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6940584.1"
                     /translation="MMGFVIKHDDFLLSHHVWFGIFIRQTVGEDGAMPIRKISEKTKI
                     RHRNEINKTLAQLPLSAKRVLCLALVSVDTKKPLEPGMIFKITADTYTSITKVKPSVA
                     YRQLKEGAELLNTSKLSLSSDDILALSTELGLPDAMRKSLERLDMGITDYCAYSPNEG
                     YVVLKFTHTIHPYISNLIGGENKFTTQLLTSAMRLSGQYSWALYQLIRKNYSKFRTKN
                     YFSIHLNELKDELIAYTIEDDEVVYKYPEFPIFKREVINKAIKEIKEKTEIEFLSCLI
                     ESKEGRKVSVLRFEFLVSEDKFTGIDNETHEFMNDDDAAFLTEFDKVVPPKKNK"
     gene            30427..30762
                     /locus_tag="ACHKV2_002722"
     CDS             30427..30762
                     /locus_tag="ACHKV2_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6940585.1"
                     /translation="MPYTAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMG
                     GNFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYL
                     QDFKKRSKK"
     gene            30759..31109
                     /locus_tag="ACHKV2_002723"
     CDS             30759..31109
                     /locus_tag="ACHKV2_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6940586.1"
                     /translation="MTATKNVKSQKNDYPASVDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
BASE COUNT         9462 a         6030 c         7063 g         8785 t
ORIGIN      
        1 aaaatgaaat gctcatctgt ttttacttca accactaatc atgttttcac gttcgaacga
       61 gtaacgcttt gcactattat ccttatgcat aaggatacgg gacaacagta tgttgtcata
      121 tttactgata acaataaaat tcgtgactat aaaaccggaa ttgttcctca gtttggcgaa
      181 cttaagcaga gtgatgttga tttagtcctt ttctacaggg acgaatatga aaaatatttt
      241 gattcgttaa aagatggcga tgaatgctta agttttaaag attttattga gtgccttcgt
      301 taagcattat gaatgcaggg gcatccctgc attttcctga aaggagttga ttttatgaaa
      361 agtgtttatg ttgttggtaa aggtttaatc gttgccggta ataaaattac catcgatgga
      421 aaagagcatg tctcggtaga aaatagtgaa aacattgttg tcactgatga ttgtattaaa
      481 attggtaata aaacagtggt ttttaaaggc aggagtatta aggtagaaat acatggaaat
      541 gtaaataaaa tagtaaccga aaatgctgat gtcagtattc atgggaatgc tggggatgtt
      601 gaaaccgtga gtggtgatgt tgattgtcat caggttaatg gcgatataac cactgtaagc
      661 ggtgatgtta cttgtggaga tgtttctggt gacgtaacca ctgtcagcgg agatataaac
      721 agaaaatgat cacggaatcg gatatgctta ctgaagctaa aaaaatggca gtagagatga
      781 tgttctggcc tgcttgtcct gtagatgatg attattgttt cgacgctata atgtatacat
      841 cagttattga caggaagttt gaagatgaac atggggtata tcttcatcag gtttacaaag
      901 aactatgctc aacacccgaa ttaaaaaagt ttctaaaaaa gaaagcaata aaattgattg
      961 cttttaagca cgatattgat cttgattcag ctaacaagat tttaatgaaa aatatgctaa
     1021 ggaggattga aaactgaaaa aaagatggta tcaggttgtg gggcaacctg ataccgtgac
     1081 acttgattct gttgcgagga ataaaatgtc ggaacgcata ttatcagcaa tacatgacgt
     1141 tgaaaagggg gggcgtccgg ttttcccttt gatgccattc catgtttttc ctgagtatat
     1201 ggcattactc agaaaagcac tggaaaagaa gacacaaaag agaacagata aataaaccgg
     1261 aggctttatg acaaaacatg aatttgtgtc ctttgtcagt ggagaattgc ggcagggcgc
     1321 tgtacgcttt tctcttgctt ttaacagcaa aggagaaatt gttctgtact ggactaataa
     1381 ggcaggaata cgagtatgga ggatattaag tggtaatcgt ggcaaaaaac caagcaaagc
     1441 taacctcgaa agaatgagta acttccggcg ctggcttttt gatgcccgtc agggcatgga
     1501 aggctacact cagcaacctg aacagagcaa ccttagctga atagccgaag ccctcccgta
     1561 tttcgggagg tgctttctga tatattgcgc gggactgcgc ttgcgcctcc tgccagaccg
     1621 ttcgcggagc gaacctgacg gcactgtcct taccgcattg attgggtttc cggtcgcaga
     1681 cgaacgtagc ttcagccatg acaacatcag cctttttgct tttttgttga tgctcaagca
     1741 caatttatga gcggtgccag attcactgcg atgcaggata gggcaaacgc cgcaaaatgc
     1801 tagcatttcg ctcgtttgcc cggtgaccta cagtcggttc ttgttggagg attttatgaa
     1861 gcaagttaat ttcagactgg aagaacaaca gcatgatgaa cttcttgatt gtcttaaaat
     1921 tctttatcca gatgagccag ctttaacagt agcgaaaggc atgaaacttt tagcaagtgc
     1981 tttattaaaa agtaaagctg gcagtaagga tataaataca ttttttgata ataaagattt
     2041 tatcaaaaca acgatgtact taacaggtaa acaaagggct gatattgaaa gagctgctaa
     2101 tcgtcacgga tggacgttat cacgggaatg tcgttaccgc atacagacga cacttgaaaa
     2161 tgaactggat ttctttgacc aggaactgct gatgatgaat cgttgccgta attcaattga
     2221 taagatcggt cgtaattttc attatatcat tgttaatgat cagaccaggg ttcttgataa
     2281 agatggtttc tatcaggatg cggagcgtct tacaacagaa atttttaatc ttaagaatca
     2341 gtttgagaat tacattatgt tatgtaaagg gagaactgtt tcaaataaag tggagatgtg
     2401 attatgggcg tttacgttga taaagaatat cgtgttaaac ggaagtcatc agaaaatggt
     2461 cgtaagtcag ctttcgctca taaagtcaaa aatggtggaa agaactatag ccgcaatgtt
     2521 caggaacgta tcaatcgcaa gggagccagt aaggaggtcg ttgtcaaaat atcaggaggt
     2581 gcgattaccc ggcagggagt tcgaaacagt attgactata tgagccgtga gtcagagcta
     2641 ccagtgatga gtgaaagcgg tcaggtatgg acaggtgacg aaattcagga ggctaaagag
     2701 cacatgatag atcgtgctaa tgatcctcag catgtgataa atgataaagg caaagaaaat
     2761 aaaaaaatca cacagaatat tgttttctca cctccggttt cagcgaaagt aaaacctgaa
     2821 gacttgctgg agtctgtcag gaaaacgatg cagaaaaaat atcctaatca ccgttttgtt
     2881 cttggatacc actgtgacaa gaaagaacat cctcacgttc atgttgtttt tcgtatccgt
     2941 gataatgacg gtaaacgcgc tgacatcagg aaaaaagatt tacgggaaat tcgtacaggt
     3001 ttttgtgaag agttgaagtt aagaggttat gacgttaaag cgacccataa gcaacagcat
     3061 ggacttaatc agtctgttaa agatgcacat aatacagcac caaaaagaca gaaaggtgtt
     3121 tatgaggttg ttgatgttgg ctacgaccat tatcagaacg ataaaacaaa gtctaagcaa
     3181 tattttataa agctaaagac tcttaacaag ggggttgaga aaacatactg gggggctgat
     3241 tttggggact tatgttcgcg cgaaagcgtt aaagcaggag atcttgtcag actgaagaaa
     3301 cttggtcaga aagaagtaaa aatcccggcg ctcgataaaa acggtgttca acatggctgg
     3361 aaaacggttc acagaaatga gtggcagtta gaaaatctgg gggttaaggg tatagacaga
     3421 acaccttcag ccagcaaaga gctggtactg aacagccctg atatgctgct gaagcaacaa
     3481 cagcgaatgg cgcagtttac gcagcagaaa gcatccacgt tacagtcaga acagaagctg
     3541 aaaacaggga ttaagttttt gggcttataa gatttaatat attgattttg caagaaaaat
     3601 atttatttta gttcgcttgc tcatcttttc gccttaaaaa cagatcgaaa ttctgcgttt
     3661 ttgccatcat tcgatctatc gatctaaaaa acagaactgc aaaagatgaa tattctgtgt
     3721 ttagttgctt atgtggaata aatgaaagaa aaattgattt atttagatcg aaagatctgc
     3781 tttatgtgtt gaaagcagca catgtggctg atagagtctt tacgagatgt gaatttatcc
     3841 ttttgttttt ttctttgctt cggggtcatc cgagtcgaga gagcgtctga aaggaaaaaa
     3901 ctatgaaatg gtatgttttg caattcacca ctacgagatt tgcagcggtt tttgcgcatc
     3961 tcgaacgcct gagcttttct tacttctgtc caatggaaac agaaaggtat cgtcgcccgg
     4021 ataagatagt ttcatatcga gaaagatgtt ttccactttt ccccggttat ctttttgtcc
     4081 aggcagattt tgaagaggtt cattctacta taataactgc tatcccttac gtacaacgct
     4141 ttatctcatt tggcggtgaa cctttacctg tatcagaaga tgtgattgag gtgcttttgc
     4201 ggagacaatc gcaatccgct caaacaaatc ttttgaggaa atcaattcca cacgaatttg
     4261 ctgaaattct ccttatggaa aatccacaac aaagaagtat ggctttcatc cattatatca
     4321 ctgagagaag cttaacccac aaaatgaaac ggaagaaaaa tgactgctac ccggaaaaaa
     4381 agcgcaacga gacgcaagcc ccaacgtaat acactctacc taccctcaac ttctcgtgag
     4441 gaactggata aaattgctct ggagattggc tatcgtaggg ggcggcgtat atcaagctct
     4501 ggtgttgtcc agtacctcat taaaaaatat tccagtcagg ctattgagga gctgataaac
     4561 gaagacgatg acgacttcga tgactaactg ttcgggctaa actttccgcg ctgttatggc
     4621 ctggagaaaa tcatgttatc tacttctact tttcttgcgc ttgccatgca atgcgctgcc
     4681 agcgttcatc ccgacacaac gcacgaagtt gccagggttg aatcaggttt taatccatat
     4741 gcgattgcgg aaataatacc aaaggttaaa cgcaaacctg gtgataaagg cgtagtatct
     4801 tactttcctg aatcaaagga ggcagcactt agtatcgtta aaaacattga atcacggaac
     4861 catcgttact ctgtaggact tatgcaaata acgagtacca attttgcaaa gttcggtacg
     4921 acagcagaga aaatgtttga tccatgcgaa aatcttaagg tatcagaaaa aatactggtt
     4981 gactgttata aacgaggtgg cgacttagtg cgcgggttga gttgctatta ttccggtaat
     5041 ccagaaacag gagtaaaacc agaacctgaa tttaataata caagttatgt acaacgtata
     5101 ggatttagcc ctcctgataa caaaaaaatt tttattgttc cctctgtaaa ggaaatgatt
     5161 aaaaaggaga acaagacgac tatcacacct gaagaaatca ttatatatcc tcaatacgcc
     5221 atacgtggca ctgtatcgaa tgaaaaggaa acaaaagatg tcgaaattaa atctgaataa
     5281 acgttattta acgctttctc tatttatggc tgcgttgatg ctttgtgttg cagaacctgc
     5341 ttttgcggat gatgtgtcta cgaagacaac tggtttttta cagaaaataa ttgatttttt
     5401 aacgaatgtt cgtaaaccgg caattacaat tatggctctt gttattggat atattgcatt
     5461 attttcgcgt caacataccg catggatagt cccactcgtt atcggaatta tcatctttat
     5521 tgttgcacca tatattccta actggcttgc gtaattaaag gatgggcgca ctatgagtac
     5581 tctttttaaa ggtcttacgc gccctgcttt aataaggggg ctgggcgttc cgctctaccc
     5641 ctttcttgga atgtgcatta tttgtgttct gcttggtgtc tggattcatg aggctatgta
     5701 cgccctcatt cttcctggct ggtatgccat caggcgtgta acacagtttg atgaacgctt
     5761 ttttgacctt ctgtatctga gaacccttgt caaagggcat cctttatcaa acaagcgatt
     5821 cagcgcagtc cattatgcgg ggagccagta cgacgaagtt gatatttcaa aagtggataa
     5881 ctttatgaag ctgaaagacc agtcttctgt tgaagagtta attccgtact cttcacatat
     5941 cactgacaac attatcgtta caaaaaaccg ggatttgctg gcaacctggc agatagacgg
     6001 tgcttacttt gagtgtgttg attctgaaga tttgtcaatt ctgacagatc agctcaatac
     6061 gcttatacgt agctttgaag ggaaatctgt tacgctgtat cctcatcgta tcagatgtaa
     6121 aaaggacgtc agaccagtat ttaacagtaa aattcctttt gtgaacagag taatgaatga
     6181 ttattacgag tcattccctc agtctgaatt tttcgagaat aaattatttc tgacgatttg
     6241 ttttaaacct tttactacgg aagataaagt aacgcatttc ttttcacgcg gtaaaaaaca
     6301 aaaagatatc tttaaagagc ctgttaatga aatgaatgaa atttgcgaca ggttgaacac
     6361 ctatctgtcc cgttttcatt cccgacgtct tgggctttat gaagatcatg gggttgttta
     6421 ttcagatcag ttatctctgt tccagtatct gctttctggt cgatggcaaa aggtcagggt
     6481 tagcagtagt ccgttttata catatctggg aggaaaagac ctgttctttg gtaatgatgc
     6541 cggacaaatt accgcgtcag accatgcccg gtattttcgt tgcatagaga ttaaggatta
     6601 ttttcaggag acggatgccg gtattctgga tgctctgatg tatctccccg ttgagtatgt
     6661 cgtgacatcg tcctttactg cgatggataa gcagtcagcg attaaggcgc tggatgatca
     6721 gatcgataag ctggaaatga cggatgatgc tgccaaatct ttgctggcag atctgaaagt
     6781 cggactggat atggtttcca gcggatatat ttctttcggg aaatcgcatc agaccctggt
     6841 tgtctttgcg gattcaccgg agcgtctggt gaaagacacc aatatcgtga cttccactct
     6901 ggaagatttg gggctgattg tcacttattc aacactgagt cttggcgcag cttattttgc
     6961 tcagcttcca ggaaattata cgcttcgccc tcgtctgagt accctcagta gtcttaattt
     7021 tgctgaaatg gaaagttttc ataatttctt ttcaggaaaa gaaaaaggaa atacctgggg
     7081 ggaaaaactg attactcttc gggggtcagg taatgatatc taccatctga attaccatat
     7141 gacgactgaa catcagaatt tcttcggtaa gaacccgacg ctggggcata ccgaaattct
     7201 cggtacgtct aacgtgggta aaaccgtatt actgatgaca aaagcatttg ccgcccagca
     7261 gttcggtacg ccggaatcat tccctgcaaa cagaaaactg aaaaaactga ccacggtttt
     7321 tttcgataaa gaccgggcag gtgaagtcgg tatacgggct atggggggat cttattaccg
     7381 ggtgaaggag ggagagccga caggctggaa tcccgccgca ctgccgccaa caaaacgtaa
     7441 tatcgctttt atgaaggacc tggtgaggct gctttgtact ctcaacagtg agccgctcga
     7501 tgattaccag aacagcctga tttcagatgc ggttgaacgt cttatgcaac ggtcagatcg
     7561 ctcttatcct atcagtaaac tacggcctct tatccaggag ccggatgata ctgaaaccaa
     7621 acgtcatgga cttaaagccc gtcttaagcc gtggacgcag ggggaagagt ttggctgggt
     7681 gttcgacaat cgggaagaca cgtttgatgt cgataacttg gatgttttcg gtattgatgg
     7741 aacggagttc ctggataata aggtgctggc cagtgctgct tcattctatc tcatctatcg
     7801 ggtcaccatg ctggccgatg gtcgcaggct tcttatctac atggatgagt tctggcaatg
     7861 gatcaataac gaagcgttca gggactttgt ttacaacaaa ctgaaaaccg gacgcaaact
     7921 cgatatggtg cttgtcgtag ccacacagtc accggatgaa ctgattaaat cacccattgc
     7981 ggcagcggtt cgtgagcaat gcgccactca tatctatctg gcaaacccga aagccaaacg
     8041 tagtgaatat gttgatggtt tgcaggtcag ggagctttat tttgacaaaa ttaaagctat
     8101 tgatccgctg tcccgccagt tcctggttgt taagaaccca cagaggaaag gtgaaagtga
     8161 tgattttgct gcttttgcca gactggagct gggaaaagca gcgtattact taccggttct
     8221 cagtgcatca aaaccccagt tagaactgtt cgatgaaatc tggaaagaag gaatgaagcc
     8281 ggaagagtgg cttgatacct atctggaaca ggcgaacctg atttgaggaa tcaccaatga
     8341 aaaagcaaat tatggcggca ttcgtcgctt cactgattgt tatttccggc gctcaggcag
     8401 ggatacctgt tgccatcgac gccaaccctg aatgggcgat tgaagccgga cgatggacag
     8461 aacgccttaa gcaatgggcg gaaacggtaa aacattacga aaatcagata aatgcgtaca
     8521 aacaggagct gctgtcaaaa acgggtatcc gtgatgtgca gggactggtg cagtccgcac
     8581 agtcagtgag tcaggaactg atgcagattt atgatcaggg gaatgctttt attgacgatt
     8641 acattaaaaa tcctgaaggg gcgttatcgg aacaggccaa atcgttattg tcggattaca
     8701 aagtaacgga tacctgccag aacctgggat attccggcga cctggtacgg ggatgtgaag
     8761 cgacgttcct ttctcaactg gcaagcgtgg aatacggtaa caagctggag agcaagcttc
     8821 gtcaggacaa ccagacgatg aaagacctta ttgatcaagt caaaaatgcg aaggatacga
     8881 aggccacgca ggatgcaaca aacgctgttg cacttgaaca actgaagttc gagaagctca
     8941 aatttcagta tcaaatgtat cgcgataagc agcgagatct tgcagaatat aaagagaaga
     9001 tggctcaggc ttctttcaga aaacagcaac gtgaagccgt gcctccttct tacagaaaag
     9061 cttatatggc aatgaaatca tatgaggatg attaaatgaa aagtatcgct actgctggcc
     9121 ttgtcattat atctgttttt agtctttctg gatgttttga agaaacaaaa tcggttgatt
     9181 ggtggcttgc acatccgaag gagacataca aaaaatttga agagtgtcag aagtctggta
     9241 gcgattctga taattgtaag aacgttaagc gagcacattt gtcttttgaa cggagaaagg
     9301 cggttggttt accaataaat tgagggtgat gtatggggat tgtcactggt ataccggatg
     9361 gtactgattt atcaggtaat gtaacctctc ccgcaaatgc gggaggtgtt cctgggtttg
     9421 attctgattt ttttcagaca actcatgaag ttatatttaa cattctcaat aagagtatat
     9481 ctggaaaatt aagtgaatat tcagatgtgg cttatactct tggtaaatat ggagtttctc
     9541 tgtatgtttt atggtatgcc tttactgtat tagcaaggaa acaacagaca cctgtacctg
     9601 attttatctg gaatatctgt aggttttaca taatattgct ttttgttaag aatacagggg
     9661 gaatacttac atcagcaaca gatgctattg atggattgaa aaatacattg gcagggggag
     9721 atccgtgggt atggatggat cagttatggg tgaaggttat acaagttgca actcttattt
     9781 ttgataaaga tacatctact gtgcctgttg ccggtgggat tggtgcttta ttaacttatg
     9841 ttggtggtgt tttggcatta ttgctttgtt ctatagtatt tgcagctgct gaattaacat
     9901 tactattact ttctgtcact gcgccaatat ttatcatgtg tttgatgttt ggtttccttc
     9961 ggcaaatgtt taatagctgg ctacagctta attttagctc gttactggtt tttctatttg
    10021 cagcattagc actaagagct gggacatggc aattaaacat ggcattaagt acgtctattg
    10081 ctacagcatc agaaaacaat cttcttcaaa cgggagtaac ttcattagct gctggcattt
    10141 tcatggcctg gattatctgg caggcgaaaa gttatgcttc acagattgca ggtgtgggtg
    10201 ttgaaggtgc catgcagggc gcagccgcta tggggattgg tgctggcatt ttcggtgcat
    10261 cccgtatggc gcgtggcgca cttggtatgg gcagaaatgc cggtattggt gcatggaaag
    10321 gtctgcgtcg tcaggaaggc gggtttggtc agtctccggg aataacgggt aagaccgcta
    10381 acctggccgg acagggcgtt aatattggtg ccaaaaagct tcgtcaggca gctattgaga
    10441 gggcaaagaa aatgtatgga gggtgattta attatgagtg ttgataccat tttaaatgtt
    10501 cttttcataa ttaacgggct tgtcgcgatc tattttcttt gttcagactg agtcttctgt
    10561 aaatattaat ctgttataca ggtgttcata ttatgaaact acttattgtg gctttcgtga
    10621 cgctttgtct cgctggttgt caggcgtcac ataaactacc gcccgtttcc gggaaaagcg
    10681 aacctgttaa ttctgctgag gtaatgcaaa atggaattta aacttcccgg atttaaaaat
    10741 aaaaaagacg ttactgactc atcagtttcg tttgaagaaa aaaacattgc actacaggag
    10801 agaatgaatc gtatttataa attcggtggt atcggaggca tgttaattgg tgggctgtct
    10861 ttacttgcat taaatgcagc attaccactg aaaacaacag ttgttgatgc ctaccttatc
    10921 gataaggtta caggtgtggc tgaacgtctg acttctgtta aaaaagaaaa tctttctgaa
    10981 aacgaagcca ttgcccgata ttttatcacc cagtatataa aacatcgtga aggttataat
    11041 tttttcagtc tccagcatga ttatgattat gtaatggctt acagcgcgga gaatgtcgcg
    11101 gcagattata acgcattatt taacagtgaa caggcaccaa aacttgttta taacaaagca
    11161 gaaaaaacgg caatggttca ggataatcca tctgtcataa tttcaccttc gtcacggacg
    11221 gatgataaag atatcggtgc gtatattcgt tttcgtctga ccatcaggga tgttgctacc
    11281 ggacaaaccc gccaggagtt ctggaatgtt cgcctgactt atcgtatcga accgcaggtt
    11341 gaaatggtgt caggggaacg taataacaat cctcttaagt tcgttgtaac aagctacgtt
    11401 cgcgataaag aagccagagg ttaataatat gaaaatgaat aaaggagcgt taattatggc
    11461 gcttctgatg gcggcgcacg tctgtaatgc tgctgttctt ccttcaggca gtcgctttga
    11521 cccacgcaat cagatagtca gttataaccc caataatacc accataatta acagtgccgt
    11581 tggatacacc accacactgg tatttgatga agatgaaaca gttatcagtg ccagaactgg
    11641 ttttccgcag ggatgggcgg ttaataaaga agataacctg gtatatctgg aagttcgtcc
    11701 tgttaaacag actgttcaga aaaataatac ggatgaaaac ggtaatacct cttctgaatc
    11761 cgtcagtgtt gctcttgacc cggaaaatga gcttgaacgc tggcgaacga atctgtttgt
    11821 tcgtaccacg aaacgtaatt acagcatgga gctgaacgcc agaacgttcc gacagccgga
    11881 gaaaattgcg tttgtggtga attaccagta tccgcaggaa cgccggaaag aacaggccga
    11941 aattgagaag aaacgcacag agactcttgc cagacgcctg gaggaacagg caatcaaccg
    12001 ttccctggaa aatgcgaaat cgccccgtaa ctggcagtac tggaagcggg ttgctgaagg
    12061 cagccaggat atcagccctg attatgcata tgacgatggt cgttatacct ggttcggctt
    12121 cagtccgtta aagaaaattc ccagcgtctt tgtgatgaac ggtatgcagg agactcttac
    12181 caatcctgtg attaaacaga gcggaagttt tacggctgtt ggcgtaccgg ttgataagcg
    12241 ttttgtttta cgtcttggtg agcaggtggt ggggattgag aaccagggct tcggaaaagt
    12301 acgtttacca gccggagata cggtatcccc ggatgttaag aaagaggtga tccagtgact
    12361 gaacaggaaa ataaaatccc gactgcaacc gaaattgaac agcagttacg ggaacgcaga
    12421 cagaaagaac tggaacaggc cgggaagacc ccggaagaag agcctggcaa gccagcattg
    12481 cagcttggta ttgaaaaact taaaaaatca cgtaaaggga tgattatcct cgtcgtgggt
    12541 tttcttctgc ttgctgccgg tgtttctgtt tattatatcc cgtccattat ccgttctgtg
    12601 tcgtcagggg atgagaaacc cgcaagtcag ccgcttgcaa ccggagaggc taaacgtcag
    12661 accggactga gcgaagatat cgatcctttt aataccgcgc agaaaaaaac agagaaacca
    12721 gaggaagaaa aagtcatttc ttctgaaaag actgaaccgc cggaaaataa acagcagagc
    12781 ttcagccgtg cacttgacgt ttctcttgat ggcagccagg caggaaacag cagcagttca
    12841 gcgggaacgt cagtttcaca tactgcggcc agtgagccag aaagcgataa aaaggatgaa
    12901 gcaaaagcaa ccgcacaggc tacagaatct gcgccactgg cgaaaataac gaaacttcca
    12961 tatgactcaa atttgtttat cccggaaggg acatcaattc cctgttcact ggacaggcgt
    13021 tttgtttctg acctggcggg gaaactggaa tgtacggtca acagtgatat atacagcgcc
    13081 agtggcaatg taaaacttat cgaaagagga accgccgcaa aactgatgta taaggccggg
    13141 tctttaaatc atggacaagg gcgtgtgttt gttatggctt ataagctacg tacccgcagt
    13201 aagcctttta ttgatattcc cctggttgac tcacaggcgg ctggcgcgtt aggcgaagcc
    13261 ggtgcttctg gctggattga cactcatttc agtgaacgtt ttcttggtgc aatgatggtc
    13321 gggatgatac cggatttaag tcaggcggcc agtggtattg ctcagaataa cagggacagt
    13381 cagacggact atacagctaa cagccgccag gcttttgctg atatagcacg cgaagcattt
    13441 tctaatagtg tgaatatccc gccaacgctt tataaaaatc agggagaaat tattactctg
    13501 attgtcggtc aggatctgga tttttcaggt atttataaac tgaaaatgaa agggggttaa
    13561 tgtgaataac gaaaacagac atctgattta tgatgtggtt aacgactatt tttatcactg
    13621 gctgaatgag actgatggtg tcacggaaat cgctgttaac cgaccaggag aaatatttat
    13681 aaaggtcagg ggaaagtggc aatggtatga acaaaagatg agttacagtg attgtctttc
    13741 ttttgcatcc acactggccg attttcatga cggcggttct gtgactcctg aatatcccct
    13801 gcgttctgcc acgcttccgg gtggagaacg tgttcaggtt gtgatcccac cggcaactga
    13861 aaaagatact gtttctataa caatccgtaa gccgtcaggt atttttatca gtcatgacaa
    13921 atttataaaa cagggatttt attcacgcgt cagtggttta agtggtgact ctgttatgga
    13981 agataatatt tctgctttaa tcacttccgg atattttgaa cgggttatac ctgaatcact
    14041 gagtcagggg aaaacgatag ttttctgtgg aggaacaggg tcaggtaaaa ccacctttgc
    14101 aaatgcctgt ctggaatata taccgcatca tctgcggtgt atttctattg aagatactga
    14161 tgaggcaaaa ttcagatttc ataaaaacca tgtaaaactt tactatccgg cagaggggga
    14221 gagtaagatt attacctcag cgagtcttct gcgttcctgt tttcgtatga atccggacag
    14281 gattctgatg acagaaatca ggggagctga ggcatgggat tttctgaaag catcgagttc
    14341 aggccatgca ggaaacatta ccaccgttca cgaaagtagt cctgaatatg ccgtgcttgg
    14401 gattgtccag cgatgttata tgaatcctga atgtcagaat ctaccattca atgtcatttt
    14461 aagacgtgta ctgagtaata ttgatattat catgagtatt aaataccttg atgatgaaga
    14521 ttttcggttc gcttccggca tttactacag acaacttcat tttgatgatt atttcagaaa
    14581 actgaaggag tgattatgtc tttaaaactc cccgataaag gccagtgggt ttttatcggt
    14641 ctggttatgt gtctcgtgac ctattatgct ggttctgttg ctgtttattt cctgaacgga
    14701 aaaacaccac tgtatatatg gaaaaatttt gattccatgt tgctctggaa aataataaca
    14761 gagagtaata tacgtacaga tatcagactg actgccatac cttctctttt gtcaggtatg
    14821 gtttcgtctc tcattgtgcc tgtttttgtt atctggcaac tgaataaaat ggatgttgct
    14881 ctttatggtg atgcgaagtt tgccagtgac aatgatttaa aaaaatcaaa actgcttaag
    14941 tgggagacag aaaatgatac ggatattctg gtcggcgcgt ataaagggaa atatctgtgg
    15001 tataccgccc cggattttgt atctcttggt gcaggaaccc gcgccggtaa aggtgccgcc
    15061 attggtatcc ctaatcttct ggtcagaaaa cactctctga ttgcgttaga tccaaaacag
    15121 gaattgtgga aaatcaccag taaggtgcgt gaaatactgc tgggtaataa agtttatctt
    15181 ctcgacccat tcaacagtaa gacacatcag tttaaccccc ttttctatat tgatttaaaa
    15241 gcagagagtg gtgctaagga tctgcttaaa ctgattgaaa ttctgtttcc gtcttacggc
    15301 atgacagggg cagaagcgca ctttaataac cttgctggtc agtactggac aggactggct
    15361 aagttgcttc atttctttat taactatgag ccgtcctggc ttaatgagtt cgggcttaaa
    15421 cccgttttct caatcggttc tgtcgtcgac ctgtacagca atattgacag ggaactgata
    15481 ctcagtaagc gggaagaact ggagggaaca aacggacttg atgaaaacgc gttgtatcat
    15541 ttgcgcgatg ccctgaccaa aatcagggaa tatcacgaaa cggaagatga acagcgttca
    15601 agcattgatg gttctttccg taagaaaatg agcctgtttt atctcccaac cgttcgtaaa
    15661 tgtactgatg gtaatgattt cgatctccgt cagttgcgac gggaagatat cactgtttat
    15721 gtcggtgtta atgcggaaga tatatctctg gcttacgatt ttctgaacct gtttttcaac
    15781 ttcgttgttg aagtgacatt gcgtgaaaat cctgattttg atcccaccct gaaacatgac
    15841 tgcctgatgt ttcttgatga gttcccttcg attggttata tgccaattat taaaaagggg
    15901 tcagggtata ttgcaggttt taaacttaaa ctgctgacga tttatcagaa tatcagtcag
    15961 ctaaatgaaa tctatggtat tgagggagcc aaaacgctga tgagtgctca tccatgccgt
    16021 attatctatg ctgtcagcga agaggatgat gccgcgaaga tatcagaaaa acttgggtat
    16081 attacaacca catcaaagag cacaagcaag agccggggac gatcagcttc acagggcgaa
    16141 tcagaaagtg aagcccgaag agcactggtg cttccacagg agctgggagc gctggacttt
    16201 aaagaagaat ttatcatcct gaagggggag aaccctgtta aagcagaaaa ggcactttat
    16261 taccttgatc cgtattttat ggacaggtta atgaaggtca gtcctaaact ggcatcattg
    16321 acgatggaac taaataagac gaaaaaaata tttggtgtga aagggcttaa atatccgtca
    16381 aaagaaaaaa tgctctccgt aggagagctg gagtctgagg ttttgctatg aaaaaaatac
    16441 ttatgatcag tattcttgtc ctcacagcct gttcctctcc acctgaaccg ccacaggttg
    16501 aatgggaaaa aatgcctgaa gttatgaata cacaaataat gaactggaca ccaacatcta
    16561 acgttattaa atcagataat ataaattcct tatggagtaa ggtgttgcct gattttaaac
    16621 cagaaaatcg tctttacgat gattctgttt tttatgcagt tgcccactct gaaaaaatag
    16681 ttgtaaggac atcttctttt gatagttact ggtcagcgaa agactggcta agaaaaaatg
    16741 gtgcaacagg tgttattgaa tatcagccac taaaaagatg gttgaataat gactatgttg
    16801 aaatttatct gtcaagaata aatgttcaga ggttaccatg aaaaagatat gcaaaggtct
    16861 aatcgtaatt tttactgtgt cctgttttat agcgccaacg tatgccgctg atccctgtaa
    16921 atccgttttt tgtctttatg gtaaagctgt cggtagtagt ggagggagtg agtgcagcag
    16981 tgcagaaaag gattttttta aaaatgttga aaagaaaaag gggaaaatcc gctggggtaa
    17041 aacatttgat ctccgtaaga attttttaaa tcagtgttca acggcagacc cggctgcaat
    17101 ctcactgatc atgagtaagt ttggtaagat taaggggtag atgatatgaa aaaaaaagaa
    17161 aagattgatt ttcttaatgc aataaaagca gggcactctg gctttatagc tggtaatcct
    17221 gctcatgcat tggatgataa gactatgaac gccatcattc ataactacga ggcaacgatt
    17281 gcgcccggta aagcaggtaa ctgcaaagaa actcctctca caaagtattg ttcgaggact
    17341 atgccagagc gtatttcagt tgaagaaatt aatgaggtta aaatttatcc agagttattc
    17401 tgaactgaaa taaaggcaaa cgccgcagat tccagatttt aatctggaat ctgcggggtt
    17461 gaacaaccga agggcgttag ttttatgatg aataaattta gttatgtcaa tcgtatgctt
    17521 cggcaagttt ttgtttttag ttttatcacg gccccatcaa tagctttatt tgttgttttt
    17581 atgttttttt cgtttaataa ctcaattgct ggttcatttt taaaagaggc cagggggctt
    17641 ataaccgata ctccttctga taaggttaac gtctgtcttt tcgaaaaggg tgtaccacag
    17701 gatatggagt tgacaaactc cacaactgaa catgtattaa aaaataaatg tataacaacg
    17761 ttagttgatg ctgctgactg gcaggaatct gttgacggtt ccattcgata tttttattgg
    17821 tcgtcagttg ttattggctt tgtggtctgg cttaccttta attttcgtgc actggaagaa
    17881 ctattttatc gtagaaagag aaaaggagac gttgaatgag gattttttta agcgtgattt
    17941 tattcctgtt tactgctttt cagtctgcaa atgcaggtgg aatacctggt gctttagcag
    18001 gaatactttc tacattaaac caggaaatcc aggggaaagt tattcgtgtt ctcgatggcg
    18061 atacaatcga agttttacag gacaaaaagc ccgtcaggat tcgcctggca aacatcgatg
    18121 caccggagaa aaaacaggct ttcgggcgct ggtcagcaaa tcagcttaaa gctctgcttg
    18181 cagcacagcc tgttacggtg acgtacacgc agacagaccg ctacggacgc attatcggtc
    18241 atgtgttcac gacgaacggc acggatgcca gccgctttat ggttcagtct ggcgctgcat
    18301 gggtatatga acgttacaac gtggatgagt cattaccggc tctacaacgg gaagcgcagg
    18361 aacaaaaacg cggtttgtgg gtcgatgcaa atccggttcc accgtgggag tggcgataca
    18421 aacataacta actgatagag gtttttatgg ctttatcacc caaagaaaag aaagagttac
    18481 aacaacgctt cgaaaagcaa ttactacctg accttgatat gtcatttgaa ctaaatggcg
    18541 aagtatataa atttaatttt gataaaaatg agtcaggtga ctatatcgat agttttacac
    18601 aagcagtatg gggaggttat caggatgccg aggaatggga aaaatatggt aaacatcagg
    18661 agacagaatt acctgaatac cctgaaccag tcgagggttg gtgcgactgt gataaatgta
    18721 agtcgatgat ttttcataaa gagaaaaata tttatgtttg cctctcctgc cataaacaaa
    18781 cagagccatt tcttccaatt gactggaact caaacgatta cgattggcta ccttatgaag
    18841 atgaatacta atgctgaata cttcacagtc ataatggatg aaatggcggc ataaaattaa
    18901 ggaggcttaa atgaatatcc atttattttc agaagtattg ttctgcgtat gggttatagc
    18961 tttgattgtg attttattta tagttgttaa atattacagg cgtgtccatt acagactcaa
    19021 ttcactgtct gaaacgataa agcgcacaca gggcggtgtt aataagcgaa tatctgaaaa
    19081 cagagaactg cttgaactta taaagaatca acatcctgaa attcttgatg aatatccgtg
    19141 ggtaagtggt tggcttgatt cacaggaaaa gtttttggtc gctcttgctg ataagtctgg
    19201 tatcgatatc aacaaatcag gacttatttg agcacactaa agataaagga aattactatg
    19261 tatatacata ttgttcctaa actttatcat ctcatggcta ataaatgtag ccttaaatct
    19321 atttccatac ctgagcttga ttttataatt gatagtgaat cattgtctgt aggcagaccg
    19381 tggcctaata aatgtttatg ggttggaatg cgaaaaggaa ggaagtcagt taatggcctc
    19441 gttttgcaaa cagataaaaa attaagatgg tttactacta tatacacctg ggatattgaa
    19501 gatatggggt taatttacca tcaagtcaat acctatatag aagatgataa ttttgatatg
    19561 gtttcacagg aaattctttt aaatggttct tttgataagt ggagtaacag ggttcattca
    19621 gcttatgaaa ataagcctcc agccagaatc cagcccaaaa tggagtcctt attaaataaa
    19681 cctggtgaaa acagtcatga tgtgtgggaa gagttcgaat ggggtgactt ccttctgtcc
    19741 agagaagaaa gccttttact tcacactatt cagtctgaac gattgagtac agattgctct
    19801 ttattcaaaa gacaaccgtc tattgaaagt gcattagtta tttaaaggat aataagatga
    19861 gactttttat cgcagaaaaa cccgcagtag caaatgatat tgttaaggca cttggtggca
    19921 attttacccg ccatgatggc tggtttgaaa gtgataacgc cattgtgact aactgttttg
    19981 gtcatattat cgaatcacaa ccgccggaaa actataatcc tgaatacaaa gcctggaagg
    20041 ttgaaacgct tcctttacgt ctttatcccg tgaagtatca gcctgttgaa agtgccgcaa
    20101 aacaggttaa aacgattctc gaacttatca gacgtggaga cgtgactgaa attgttcacg
    20161 ctggcgatcc tgatgatgag ggacagttac ttgttgatga agtcctggaa tatgcaggca
    20221 acacaaaacc cgtaaagcgc gttctgatta acgacaacac gcttccggca gtgaaaaagg
    20281 cactggcaaa tcttaaagat aatcgtgatt tcaaagggct ttaccttaag gcgctggcgc
    20341 gttcagttgc tgatgccgtc tatggcttct ccatgacgcg tgcttacacc attcctgcaa
    20401 aagccagagg atatcagggc gttctgtctg tcgggcgcgt ccagactccc gttcttggcc
    20461 tgattgtgaa tcgtacccgt gctaaccaga accataaatc cagtttttac tacaccatga
    20521 ccggagtctt tcagcgtggt gctgatgtta taagggcgaa ctggaagcca ggggaatttg
    20581 ctccgctgac agaccgtaaa ttacttgata aagcgtgggc agacggaacg gctgcatccc
    20641 ttgcaggaaa accggctaca gttgaagcgg cagcaactga tgataaaaaa acggctgcac
    20701 cgttgccctt taacctggta cgactccagc aatacatgaa caaaaagttc aaaatgacgg
    20761 cacaaaaaac gctggatatt acgcaacaat tacgtgaaaa atataaagcg attacttata
    20821 accgctctga ttgctcatat ttatctgatg aacaattcag cgaagcgccg caggttatcg
    20881 atgccctgaa atcagtcttt cctcagtcgc tggatattga ttcttcacgt aaaagcaaag
    20941 cgtttaacag tgcaaaggtg actgcgcata ctgctataat cccgaccgcc agtgtgcctg
    21001 atgttaacgc actcagcacc gacgagcgca atgtttacct ggcgatcgca caacactatc
    21061 ttgttcagtt catgcctgaa aaagcatacc aggaagtatc ggttgccatt cagtgtggtg
    21121 atgagtcgtt ctatgcccgt gccagaaaaa caactgacag cggatttgag gcgtttcttg
    21181 gcgcggaaac cacagacgaa ggtgaatcag aagataatga tgattccgct tttgaactgc
    21241 tctgtaaaat tcgcacagga gaaacactga cgacaaaaga agttattgtt aatgagaaga
    21301 aaacaacacc gccgccgtta ttcaccgaag cctccttgct tgctgcgctt gttcgtgtcg
    21361 cggattttgt cactgaccca acgattaaaa aattgttgaa ggataaggat aaagacaaaa
    21421 aagatgaaca tggtggcatt ggtacgccag ctacccgcgc agccattctg gaaacgctga
    21481 agaaaagaaa ctatatcacg ctggaaaaag ggaaacttat tcctactgat accggatatg
    21541 cgcttattga tgccctgcct ggtatagcgg ttaatcctga tatgacagca ttatggtctg
    21601 aaaagcaggc tgccatagaa aatggcgatc tgacggttga acagtttatt aatgatctgt
    21661 acggtgaact gacaggcatg atttctgatg ttgacctggg cgagatgaag attgaacccg
    21721 ctgcgccagc agggcagttt caacgcctgg actctccctg cccttcctgt ggtaaacata
    21781 ttgttatcag gccgaaaggt tatttctgta ccggatgtga atttaaaatc tggagtgagt
    21841 tttatggtaa gaaaatcacc caggcacagg ccgaaaaact ggttaaatca gggaaaaccg
    21901 atttgattaa gggatttaaa aagaaaagtg gtggaacgta tgatacagtt cttgtccttg
    21961 aggataagaa aacagggaaa ctgggttttc cggcaagggc taagaagtga aaacaaagca
    22021 ggaatggctt tttcagttaa gaaaatgtac atcaagagat actcttgaaa aagttattga
    22081 gattaaccgt tacaaactgc ctttatcaga atcagaggca ttttattctg ccgcagatca
    22141 tcgccgagca gaactggtga tgaataaact ttatgataag gtgccttccg gcgtctggaa
    22201 gtacgtacac taaataagag gattaattat gagcgaactg actaaagaag atgaatacgg
    22261 cattatcagc cgtactatga tgaatattcg ttcattgcgt gtttttgccc gtgaagttga
    22321 ttttgagcaa ttgctcgaaa tgcaggaaaa actgaacgtt gttgttgaag aacgtcgtga
    22381 agaggctgaa cgtgaagcgg ctgaacgcca ggaacttgaa gccaaacgtc agcaggccat
    22441 tgaatacatc atatctttag gtcttgatcc cgaatcactc cttgctcctg taacagcaga
    22501 tacagtaaaa accaggcgga aagccaaagg cggagtaaga aaggctaaat accgctttaa
    22561 ggatgaaaat ggcgagatca gagaatggtc tggtaatggc aagcgtccgc ttgctttaca
    22621 gaagctgctt gatgagggcc atttcatgga agatttcctc attgacaaac tgcaacctgg
    22681 gcaggttgaa taaaataatc gtgatgccgt cgcaaacaag tttgcgacgg caaatggagg
    22741 ggacagaaca gccagcggtg ctggcaagaa attttatcgt ttgataaaga gggaaaatga
    22801 tgatcaaatc aaaaaaagta gtggttattt ttgttgcttt gagttcattc ttgctggcag
    22861 ggtgtgattc accaaaagat gctaataagc aaaactttga aaaagctatt aatactcgac
    22921 tagagaagga atgcattact attagtccgc tgggaatgtt tgatagtaag ccttatccgg
    22981 ttcaggtagc tgtggctcag gctaatcgat atacatctca ggaacgggca gatgaaataa
    23041 attctcgaca gttttctgcg ctggatgtac tggtcaaagc cgatttatta acagtaaaag
    23101 ataccctggt tgatgacgtt attggtttta ctaagacggg taagaaagtg ccaggtcgtg
    23161 aatatgctct gacagatgag gggaaaaaat atctgaagtc tccggaacgg cctgattttt
    23221 gcgttggtca ttataaggtg gatgagatag ttgattttac cgaacctggt gatgctatgg
    23281 gaatgaagat cacgcaggtg aattacacat tcagtcccac cagtatcgct gaatgggcga
    23341 aacgcgatga tgttcgggct gcatttttag gacttgagtc tgatttgaaa gagaaacaaa
    23401 caaaacgtat tacactggtt ttgaaaaatg atggatggtc tgctgaacgg taggctgtaa
    23461 tggtgttgct acgtgaaaaa taccccacgt atatccccta ctttttcacg gatttcacgt
    23521 agcgcaccgc cgttaatgac tctatcaaaa atactcaccg atatttttca caccgcaaac
    23581 aacctaactt actgtaaaac aacaaatcag ctttgcgccg gtgacgcatc gatcgcgctg
    23641 gccagcgcga tcctcagctg cgaccgctac agtatttttg aacaagatta cccttgctcc
    23701 ggtttaattc gccaggcagt tagacataca ggtcattctg tccagatttc attagttaca
    23761 acattcacat gttccgttcc tttatccggt gccctgttcc ggtctatgtg cccatatcct
    23821 gggttattca ggataaccgc ttccttttcg ccctgccctg tcgttcatat ccccctggac
    23881 aaaaatgcat tatcggaaaa aatgtccaaa aatttttttg gtgatcggaa tttttgttgt
    23941 cggacgcacc tctgcttcag gtcataattg ctggcacgtc caaaaaaaca ggtgcggaga
    24001 ttaaaaaatt gagtcattta cccgccagca ttttccagat ttcacgtagc gacgcctgga
    24061 atttaaccct ctctcccgat gcagcccgta atatgcggga tctggcggcg cagattggcg
    24121 caggaatgcc aaaatatctg atagcaccgg aagtctcact gcttctctcc tacattgatg
    24181 atctgagcat ccgcatgtac tttgagacac tctggaatac cggagcacga ctgaatgaag
    24241 ctctggcact gaaacctgct gattttatgc tgactccgac gcgccagtac ccacaacccg
    24301 tggtgatcct gaaaacactc aagcaacgta taaaagaagc agaaagaaga ccaggacgcc
    24361 cgaccaccag gcacccacca ttacaccctg atccgcgata cagaaaacca ccggagcctg
    24421 ctgtaaggct ggttcctgtt ctggatgccg gatacgcatt gcgtatgcgg gaatacctgg
    24481 cgacctggaa aaagcggatc aaacatcagc cagtgtggga cataacatcg agacagacac
    24541 cgctgaactg gataaacacc gcagtggcac gggcagggcg tgacggcgta acattttcgg
    24601 ttccggtcac gcctcacaca tttcgccaca gttacgccat gcacctgaca atgagtggcg
    24661 ttcctccgcg cgtcctgcaa tccctgctgg gacaccgtta tgcaagaagc accgagattt
    24721 acacccgcgt tttttcgctg gacgtcctta caggaagtgg tctgtccttt acctgcgatc
    24781 cacagcttgc aaaacagctt cttggatacc ctgatgcgcc ttaataatga gtaataatac
    24841 acaccattaa actattgcca gcacgcaatt gtacggttat actttcatca cataaaattg
    24901 tgtattaata cacaccattt aaccaagagg attttgtgat gggaaaaatc attacagttg
    24961 cagggcataa aggcggcata ggaaaaagca ccgtgctttg ttccttatgt gtgtgcgtca
    25021 tcataaaagg gaaaacagcc tgtttcctgg aaacagactc acagggaagc atcaaggatt
    25081 tcatcgagga acgtaaaact aacgaccggc tgtctgaaat accgtatttc gagtgctaca
    25141 cagatattcc ggcaatggca cgaaaactgg ctgcccgctt tgattatgtg tttgttgata
    25201 cgccaggaat gaaatctccg gccttcgtta aggcactgag ttgtgctgac attctgttca
    25261 cattcattga gccaggagca ggcatcgaga tcaacacgct gggacggctg gttttcgaca
    25321 ttaaaacagc acaggccgga gtcaatccgt caatgaaagc atggattgtc ctgaataagt
    25381 gttcaactaa cccgtcagac agtgaagcgt ctgaactgcg ccgacaactc aatgatgatc
    25441 cggactggct tccggttccc cgccagcgta tctatatgcg tactgtgcat aaaaaagcct
    25501 ataacagtgg tatgggcgtg catgaatacg atgataaacg gggaaacaaa gcccgtggag
    25561 agattgaatt actgttaaaa gaaacggatg ttctgtaatg tgtattatta cacactatag
    25621 gcgatgatat acttacatca ttaatagtgt gtataattac acaccttaca aaaaacagga
    25681 taccgaatct aatggcaaag ctcccaaaga aaattgatca ggaaattaaa ccgctggatt
    25741 tacgccagtt tgtaagttct gctgcacaaa aaccagcatc ggcatccgca gtaacgcgta
    25801 taaatctgac tatgacagat gatgatctgg atacagcgca ggtgttccag aacaagtacg
    25861 gtgcaagccg tgctgaagtt atcagagcag cactggcagc gttacgtctg gtatcagcag
    25921 aagaacgccg gaggctgttt gatgatataa ggaaaaacag cccgaaagca ggaagaccaa
    25981 ccgtgaataa atagtgtgtt ttattacaca ctattaaagg ggataaaaat atgctcataa
    26041 agatcattaa atctgtattc aatttttaca tgctctgtgt gcagccttta cttggattat
    26101 ttctcatggg ttacagtata tggagcttct ctggtgacgg tatctgtacg gcagacaaag
    26161 ttatagcctg tgatatatca atatatctgg aaccagcatt ttaccttctc attggcactg
    26221 ttgttttgtt tgtatggtat atagcatggc agacagaacg ctcgaaagaa gaagatttta
    26281 aatcaagcat taagccggaa gattttgtga agtgagatta tggttgttga taaattgtat
    26341 gcagcattag atatgcaact aaaattgatt gcgacggtgt tataaagaac attcctgtaa
    26401 gcacatataa caaactaata tacgcctata ataaaagttc attcttaaca gatacagaat
    26461 aagaggaaat gccattatga gtgaaatata ctgcattgca attgcagtca ttatcgttgg
    26521 ttacgtttat ctaatgatca gtgtaattac tgcaccacaa aaattgtttc ctatacttca
    26581 gctaataact ctaataggta tcagttcgtt attttctttt atgtcattca agagcgaaag
    26641 catgtggacg ggtgagcctg aattattagt tgcgattatg tttggcactt tttcagcaac
    26701 actgattttt tacatgttta aaattttgat tcgcgctttt gattcattga ctaaataaca
    26761 aagctgaagt atatagcatg gcaaaaagaa cgttctaaag aagaagagtt taaatcaagc
    26821 ataaaaccag aagattttgt gaagtgagga tacggaataa tgccagctta taaccagaca
    26881 ttagggcact ttaggggatt ttcaatctat ggaacactgc atcgttgatt taggcattat
    26941 aaactatcat cctgtcatta catggacagt acctgttgca tttttctttt tagtagtgct
    27001 ggctggtata gcattcaata ttggcaggaa aagtaatcta atcgttttca tcctggcaat
    27061 gttgctccca gtaacaactg ttttcatttt tatgttactg ggcggtaaga tcgatatgtg
    27121 ctgatcaatt aaaagaggat tatacaatga taaattcaat cgcgtttagt cttatagcgt
    27181 tatgtataat atatatatca tcactactta aaaaaagtaa cttcagaata ttttatataa
    27241 ctgtatctac gtgctcttta ttgttgtcat gtgtaccaaa caagtacagt atgattggta
    27301 tagcagcatt ggtatttaca actatgttag gaccattatt aattatctta gaggataaca
    27361 ggcaataaat tatgataaag catctgttaa gcccgcaaat atgtgggctt atttttaatt
    27421 actcttcatc atcccgcagg aactgacaaa agtcttcact ccccatcata tcgacagtgt
    27481 ttttaaattc gaaattacag cccatttcag ttgtataatg aatccagcgt ttcattagtc
    27541 agcaattcag acaggttctc aacaaccaaa acattaatat caaattgtcc gacaaaaaca
    27601 gaaccagcaa cttgctcaaa gctgatgata gctacaggct tacgttcatt catttcaaaa
    27661 ataattaatg gtaaagtaat actatttatt gattcaaact ctttaaagaa tgcatcagtg
    27721 ccatactcac tggcaggaaa aagaatctca cttaatctta ctccataaaa aacatgatag
    27781 ttttctttaa ggttatcctt taaaatgtta atagccttag aatatttttc attaacaaac
    27841 tctttcacct gatatttcat aaaattctcc aacaataacc gactgtaggt caccgggcaa
    27901 acgagcgaaa tgctagcatt ttgcggcgtt tgccctatcc tgcatcgcag tgaaaaagca
    27961 ggaactggca taacggctga gtagcatcgt tactggctta tctcgctact gttctcggtg
    28021 ctttcgtccg caggttcggg aacaagtaac ttgtgccagg gcatagtttt gttgagcagc
    28081 tccatcagag gctcagccag cagtcccaaa atccaagttg ccgtaatcac cggtatggtc
    28141 agattttgtg gcgtccataa ctggtgatcg agatgatacc aggtgatgac acacaaaatc
    28201 ccccccagcc agaacacagc ccatttaaac gcaccaagaa tggctaacac aaaaacagag
    28261 acaaaatgta aaacactggc ggccagaaga cgaagataaa accatcccca gccaagccac
    28321 ttaacaaaaa tgctccctgt tttcaccgca aaacgtttct tacgttctct ggcgatttct
    28381 ttcctgaatt taccgcgttc ctcccccagg gggaattgat agattttagc catgattcac
    28441 ctcacttatt cttttttggt ggaacgactt tgtcaaactc tgtcaggaaa gcagcatcat
    28501 catcattcat aaattcatga gtttcgttat caatacctgt aaatttatct tcagaaacaa
    28561 gaaattcaaa gcgtaaaaca ctaactttac gcccctcttt actttctatt aagcaggaca
    28621 ggaattctat ttccgttttt tctttaatct cttttattgc tttattgatc acttctcttt
    28681 tgaatatagg aaactcagga tatttataaa caacttcatc gtcttctata gtatatgcga
    28741 ttaactcatc ttttaattcg ttcagatgta ttgaaaaata attctttgtt ctaaatttgg
    28801 aataattttt tcttattaac tgatataaag cccatgagta ttgaccactt aatctcattg
    28861 ctgaagtaag caattgagta gtgaatttat tttcaccgcc aattagattt gaaatgtaag
    28921 ggtgaattgt atgcgtaaat tttagaacga catacccctc attcggacta tatgcacaat
    28981 aatcagtaat ccccatatcc aggcgttcta aactcttacg cattgcatca ggtaagccta
    29041 attcagtaga taatgccaaa atatcatcac ttgatagtga aagtttacta gtattaagta
    29101 attcagctcc ttcttttaac tggcgatatg caactgaagg tttgactttt gtaatagatg
    29161 tataggtatc agcggtaatt ttaaaaatca tacctggttc aagcggtttt ttcgtatcaa
    29221 cagacaccaa tgccagacac aaaaccctct ttgcagaaag aggtaactga gcaagcgttt
    29281 tattgatctc atttcgatgt ctaatttttg tcttctcaga aatctttctt attggcattg
    29341 ctccatcctc acctactgtt tgtctaataa atataccaaa ccaaacatga tgactcaaca
    29401 aaaaatcatc atgtttaatg acaaatccca tcatgtttaa cacagcaatt ttgagggagg
    29461 atgctacagt gagcagctaa acagtgaaaa catgtttaaa acatgaactg atcacctgaa
    29521 gatcggaaga tctgaagtaa aagaaccaat cggttaataa tgtgacctaa accatcatgc
    29581 atagtgacct aaaccatcat ggatagtgac ctaaaccatc atggatagtg acctaaacca
    29641 tcatggatag tgacctaaac catcatggat agtgacctaa accatcatct ttagcgtttt
    29701 tttattatat ttatcataat gttacagcga tctaagtttt taagttttta agatcaagaa
    29761 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa acccggagga
    29821 tagatcactc tgaaccagga caaccacaca taaaaacact atgctcgctt tttaaccact
    29881 gcgcgtaagt gcctgatgct agcccgacgc aattccgctt cctcgctcac taactcgcta
    29941 cgctcggtcg ttcggctgcg gcgagcggtg tctcctttct caaaacctga agagttcaga
    30001 tcatacgatc cgttgattga tcctttttgg atctttcact gagaagccct gaatcgctct
    30061 ctgccgcttc gttcgataca accatgagga aaacatgcga aatgaaaaaa atcgcttaaa
    30121 acgcgataca gcgcgtttta gggggtattc acaaaacaca cgttgccttg aagaaatttt
    30181 gatcaagatt taactactat ccggatcatg acaacagatc atagcaacaa ggctgctaaa
    30241 aagcgttgag agacgttaaa cgctgttaag actagggcta agtacaatga aacggaaaaa
    30301 atgctcagaa tgcgttacag aaccttccag aaagctaagg tttgtcactt taaagagaca
    30361 tttttcttga tctagtgtca cttataagag acaatgtaca caggttaagg gaatgagggc
    30421 ggcaagatgc cttacacggc gaaacgatat cagacagaaa acggtgaggt tccgtacacc
    30481 gactggatga aaaaattaag acgtaaagac cagaccgcag cgttaaaggt ggattccaga
    30541 attgcccgcg ctatgggcgg aaattttgga gatcataaat tcgaaaggga cggtgtatgg
    30601 gaattacggg ttgattacgg tcctggttac agggtttatt attcaatcga agatggcgaa
    30661 ataattcttc tgctcattgg agggaataaa aaaactcaga cagccgattt agataaagcc
    30721 gttagttatc tgcaagattt taaaaagagg tcaaaaaaat gacagccacc aaaaatgtga
    30781 agtcgcagaa aaatgattat ccggcatccg ttgatcacaa tgccgccatg attgaagaat
    30841 tgcgcgcaga tcctgattat gcaaacgctt atcttgctaa tgccctggaa gaaataaacg
    30901 agcctggtgg tttaggtggt tttcttgttg cactacgtca ggtaatcgag gcgcgagggg
    30961 ggatttccga agctgccagg aaatctggcc ttgcccgtca gagtatttac cgcgcattat
    31021 cccccaacgg taacccgacc atcacaacgc ttgcacaact tacatcggta gccggattac
    31081 agttcactct cagtaaatcc agccactaat ctctccagtt ccccctaagc gggggaattt
    31141 ccatgccatc aaaaccgcag ttatcccgcc cgacgttcac cggtatttaa tcggcaaatc
    31201 gtacaattgc gtactggcaa tagtttaaca gtgtgtatta atacacattt aaagacgatg
    31261 tgatagatgc gtgtgttaat gctaatgtat gcatacatta gcattaacat agagcagatt
    31321 ctggcgcgtt gcgcccgtag
//
LOCUS       DAZFTN010000056        31127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFTN010000056 DAZFTN010000000
VERSION     DAZFTN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..546
                     /locus_tag="ACHKV2_002724"
     CDS             <1..546
                     /locus_tag="ACHKV2_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO6940587.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
     gene            776..1159
                     /gene="secE"
                     /locus_tag="ACHKV2_002725"
     CDS             776..1159
                     /gene="secE"
                     /locus_tag="ACHKV2_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO6940588.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1161..1706
                     /gene="nusG"
                     /locus_tag="ACHKV2_002726"
     CDS             1161..1706
                     /gene="nusG"
                     /locus_tag="ACHKV2_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO6940589.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1865..2293
                     /gene="rplK"
                     /locus_tag="ACHKV2_002727"
     CDS             1865..2293
                     /gene="rplK"
                     /locus_tag="ACHKV2_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO6940590.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2297..3001
                     /gene="rplA"
                     /locus_tag="ACHKV2_002728"
     CDS             2297..3001
                     /gene="rplA"
                     /locus_tag="ACHKV2_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6940591.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3293..3790
                     /gene="rplJ"
                     /locus_tag="ACHKV2_002729"
     CDS             3293..3790
                     /gene="rplJ"
                     /locus_tag="ACHKV2_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO6940592.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3857..4222
                     /gene="rplL"
                     /locus_tag="ACHKV2_002730"
     CDS             3857..4222
                     /gene="rplL"
                     /locus_tag="ACHKV2_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO6940593.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4542..8570
                     /gene="rpoB"
                     /locus_tag="ACHKV2_002731"
     CDS             4542..8570
                     /gene="rpoB"
                     /locus_tag="ACHKV2_002731"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO6940594.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8647..12870
                     /gene="rpoC"
                     /locus_tag="ACHKV2_002732"
     CDS             8647..12870
                     /gene="rpoC"
                     /locus_tag="ACHKV2_002732"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO6940595.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13083..13526
                     /locus_tag="ACHKV2_002733"
     CDS             13083..13526
                     /locus_tag="ACHKV2_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO6940596.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="ACHKV2_002734"
     CDS             complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="ACHKV2_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO6940597.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="ACHKV2_002735"
     CDS             complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="ACHKV2_002735"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO6940598.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="ACHKV2_002736"
     CDS             complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="ACHKV2_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO6940599.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="ACHKV2_002737"
     CDS             complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="ACHKV2_002737"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO6940600.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="ACHKV2_002738"
     CDS             complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="ACHKV2_002738"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO6940601.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="ACHKV2_002739"
     CDS             complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="ACHKV2_002739"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO6940602.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19406..19506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="ACHKV2_002740"
     CDS             complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="ACHKV2_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO6940603.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20129..20902
                     /gene="nudC"
                     /locus_tag="ACHKV2_002741"
     CDS             20129..20902
                     /gene="nudC"
                     /locus_tag="ACHKV2_002741"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO6940604.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20942..22006
                     /gene="hemE"
                     /locus_tag="ACHKV2_002742"
     CDS             20942..22006
                     /gene="hemE"
                     /locus_tag="ACHKV2_002742"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO6940605.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22016..22687
                     /gene="nfi"
                     /locus_tag="ACHKV2_002743"
     CDS             22016..22687
                     /gene="nfi"
                     /locus_tag="ACHKV2_002743"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO6940606.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22730..23320
                     /gene="yjaG"
                     /locus_tag="ACHKV2_002744"
     CDS             22730..23320
                     /gene="yjaG"
                     /locus_tag="ACHKV2_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO6940607.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23507..23779
                     /gene="hupA"
                     /locus_tag="ACHKV2_002745"
     CDS             23507..23779
                     /gene="hupA"
                     /locus_tag="ACHKV2_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO6940608.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23792..24487
                     /gene="yjaH"
                     /locus_tag="ACHKV2_002746"
     CDS             23792..24487
                     /gene="yjaH"
                     /locus_tag="ACHKV2_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO6940609.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="ACHKV2_002747"
     CDS             complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="ACHKV2_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO6940610.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25152..26528
                     /gene="zraS"
                     /locus_tag="ACHKV2_002748"
     CDS             25152..26528
                     /gene="zraS"
                     /locus_tag="ACHKV2_002748"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO6940611.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26525..27850
                     /gene="zraR"
                     /locus_tag="ACHKV2_002749"
     CDS             26525..27850
                     /gene="zraR"
                     /locus_tag="ACHKV2_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO6940612.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQCVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27847..29136)
                     /gene="purD"
                     /locus_tag="ACHKV2_002750"
     CDS             complement(27847..29136)
                     /gene="purD"
                     /locus_tag="ACHKV2_002750"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO6940613.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     NKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29148..30737)
                     /gene="purH"
                     /locus_tag="ACHKV2_002751"
     CDS             complement(29148..30737)
                     /gene="purH"
                     /locus_tag="ACHKV2_002751"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO6940614.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7310 a         8054 c         8522 g         7241 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg
      541 agctaattgc cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt
      601 tttgcacgct ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc
      661 tgagacaggc tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt
      721 tgcctcgcga tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag
      781 tgcgaatacc gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt
      841 ggtggcattg ctcttggtgg cgattgtcgg caactatctt tatcgcgaca ttatgctgcc
      901 gctgcgtgcg ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac
      961 gacaaaaggt aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt
     1021 catttggccg actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc
     1081 agtaatgtca ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat
     1141 cactggcctg aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg
     1201 cgttttccgg ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca
     1261 acatggaaga tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg
     1321 gcggtcagcg tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg
     1381 tgatgaacga cgcgagctgg cacctggtgc gcagcgtacc gcgtgtgatg ggcttcatcg
     1441 gcggtacttc cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc
     1501 gcctgcagca ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg
     1561 tccgtgttaa tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg
     1621 agaaatctcg tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg
     1681 acttcagcca ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg
     1741 cacaaggcgc gagattggaa tacaatttcg cgccttttgt ttttatgggt cttgcccgta
     1801 aaacgatttt ttatatcacg gggagcctct cagaggcgtt attacccaac ttgaggaatt
     1861 tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa
     1921 cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg
     1981 caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat
     2041 caccgtttac gctgaccgtt ctttcacttt cgttaccaag actccgccgg cagcagttct
     2101 gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg
     2161 taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg
     2221 tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt
     2281 agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt
     2341 tgatgcaacc aaacagtacg acatcaacga agctatcgct ctgctgaaag agctggcgac
     2401 tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc
     2461 tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccgtt ccgttcgcgt
     2521 agccgtattt acccagggtg caaacgctga agctgctaaa gctgcaggtg ctgaactggt
     2581 aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat
     2641 tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg
     2701 cctgatgcca aacccgaaag tgggtactgt aacgccgaac gttgctgaag cggttaaaaa
     2761 cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat
     2821 cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc
     2881 gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc
     2941 caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta
     3001 atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtatg ctaacgaaag
     3061 ttagcagcta aaaagatttg ttcgttggag cttggcctat ccaggcctcc gtcgaagacc
     3121 gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat
     3181 tcttttatat tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg
     3241 agtgaagtga gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt
     3301 aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct
     3361 gtctgcagta gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa
     3421 agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc
     3481 tgttgaaggt actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc
     3541 atactctatg gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa
     3601 tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat
     3661 cgaccgcctg gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat
     3721 gaaagaagct tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga
     3781 agctgcttaa tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata
     3841 ttcaggaaca atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat
     3901 gtctgtaatg gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc
     3961 tgctgctgct gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt
     4021 cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg
     4081 cgcaactggc ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct
     4141 gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg
     4201 cgctgaagtt gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga
     4261 tggctggtga ctttttagtc accagccttt ttgcgctgta aggcaccagt agcgtttcac
     4321 actgtttgac tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc
     4381 gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc
     4441 attgcggaaa gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca
     4501 atggacagat gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg
     4561 agaaaaaacg tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc
     4621 tcctttctat ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt
     4681 atggtctgga agctgctttc cgttccgtat tcccgattca gagctacagc ggtaattccg
     4741 agctgcaata cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa
     4801 tccgtggcgt gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc
     4861 gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa
     4921 ttccgctcat gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt
     4981 cccagctgca ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt
     5041 cgggtaaagt gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg
     5101 aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga
     5161 ccatcattct gcgtgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa
     5221 aagttatctt tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc
     5281 gtggtgaaac cgcatccttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc
     5341 gccgtatcac tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag
     5401 tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg
     5461 gcgagctgat ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc
     5521 agtctggtca caagcgtatc gaaacgctgt tcaccaatga tctggatcac ggcccgtata
     5581 tctctgaaac cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct
     5641 accgcatgat gcgccctggc gagccgccga ctcgtgaagc agcggaaagc ctgttcgaga
     5701 acctgttctt ctccgaagac cgttatgacc tgtctgcggt tggtcgtatg aagttcaacc
     5761 gttctctgct gcgcgaagaa atcgaaggtt ctggtatcct gagcaaagac gacatcattg
     5821 atgttatgaa aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc
     5881 acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg
     5941 gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc
     6001 tgatgcctca ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg
     6061 gttccagcca gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca
     6121 aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag
     6181 ttcgagacgt acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc
     6241 cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc
     6301 ttgagactcc gtatcgtaaa gtgactgacg gtgttgtaac tgacgaaatt cactacctgt
     6361 ctgctatcga agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag
     6421 gccacttcgt agaagacctg gtaacctgcc gtagcaaagg cgaatccagc ttgttcagcc
     6481 gtgaccaggt tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc
     6541 tgatcccgtt cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac
     6601 gtcaggccgt tccgactctg cgtgctgata agccgctggt tggtactggt atggaacgtg
     6661 ctgttgccgt tgactccggt gtaactgcgg ttgctaaacg tggtggtgtc gttcagtacg
     6721 tggatgcttc ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag
     6781 gtatcgacat ctacaacctg accaaataca cccgttctaa ccagaacacc tgtattaacc
     6841 agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc
     6901 cgtccaccga cctcggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt
     6961 ggaatggtta caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc
     7021 gtttcaccac catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc
     7081 cagaagagat caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactggatg
     7141 aatccggtat cgtttatatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg
     7201 ttacgccgaa aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg
     7261 gtgagaaagc gtctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta
     7321 cggttatcga cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg
     7381 aaatcgaaga aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc
     7441 tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg
     7501 agaagctcga caaattgccg cgcgatcgct ggctggagct gggcctgacc gacgaagaga
     7561 aacaaaatca gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga
     7621 agaaactcga agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc
     7681 tgaagattgt taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg
     7741 caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt
     7801 acgatgaaaa cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta
     7861 tgaacatcgg tcagatcctc gaaacccact tgggtatggc tgcgaaaggt atcggcgaca
     7921 agatcaacgc catgctgaaa cagcagcagg aagtcgcgaa actgcgtgaa ttcatccagc
     7981 gtgcgtacga tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg
     8041 aagaagttat gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt
     8101 tcgacggtgc gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt
     8161 ctggtcagat ccgcctgtac gacggccgca ctggtgaaca gttcgaacgt ccggtaaccg
     8221 ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt
     8281 ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg
     8341 gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc
     8401 tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg tcgtactaag atgtataaaa
     8461 acatcgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt
     8521 tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca
     8581 aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc cgacgggagc
     8641 aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat
     8701 gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt
     8761 aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc
     8821 gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg
     8881 aaacaccgtg gcgtcatctg tgagaagtgt ggcgttgaag tgacccagac taaagtacgc
     8941 cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa
     9001 tcgctgccgt cccgtatcgg cctgctgctc gatatgccgc tgcgcgatat cgaacgcgta
     9061 ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga gcgtcagcag
     9121 atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg
     9181 aaaatgggtg cggaagcaat ccaggccctg ctgaagagca tggatctgga gcaagagtgc
     9241 gaacagctgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc
     9301 aagcgtatca aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc
     9361 ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt
     9421 cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt
     9481 ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg
     9541 ctgcaggaag cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt
     9601 tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc
     9661 cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtgggt
     9721 ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa
     9781 ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag
     9841 aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa
     9901 cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa
     9961 ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac
    10021 gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg
    10081 gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca
    10141 atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg tgactgtgtt
    10201 aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc
    10261 tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat
    10321 gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt
    10381 ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa
    10441 aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt
    10501 atttttgcgg accagatcat gtacaccggc tttgcatatg cagcgcgttc tggtgcatct
    10561 gttggtatcg atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa
    10621 gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc
    10681 tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg
    10741 gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa gcaggtttcc
    10801 ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt
    10861 cagcttgctg gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca
    10921 atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt
    10981 gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta cctgactcgt
    11041 cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa
    11101 ggtatcatga tgactccggt tatcgagggt ggtgacgtta aagagccgct gcgcgatcgc
    11161 gtattgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt
    11221 ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac
    11281 gcggttaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc
    11341 tacggtcgtg acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc
    11401 gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt
    11461 ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc
    11521 aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt
    11581 aacaccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct
    11641 tacggtgcgg tactggcaaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca
    11701 aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact
    11761 gacatgatcg acggccagac cattactcgt cagaccgacg aactgaccgg tctgtcttcg
    11821 ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg
    11881 aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat gcctgcgcag
    11941 tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt
    12001 gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg
    12061 ccacgcgttg cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa
    12121 atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct ggttatcacc
    12181 ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg
    12241 ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac
    12301 gacattctgc gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa cgaagtacag
    12361 gacgtatacc gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt
    12421 cagatgctgc gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa
    12481 caggttgaat actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg
    12541 ggtgcaactt actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc
    12601 ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg
    12661 ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg
    12721 gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct
    12781 ccggctgcac cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac
    12841 gcaggtctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
    12901 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
    12961 gaatttctga atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact
    13021 aattgagtca aactcggcaa ggattcgata ctattcctgt gtagctttct taaggaacga
    13081 gaatgaaaca ggaagtggag aagtggcgac cttttaaaca tccggatggc gacgttcgtg
    13141 atttatcgtt tcttgatgct catcaggtta tctacgttca gcatcatgag gacaaggagc
    13201 ctttagagta tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac
    13261 atcagacgaa cgaagaaaaa ctatcgttaa tgtaccacgc gcctaaagaa tctcgtccct
    13321 tctgccagca ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc
    13381 cagaaagcaa cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag
    13441 acggaggaga taaggcattt tactttgttg tgttcagggc tttcagtgaa atttttcacc
    13501 attgcctaaa acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    13561 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    13621 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    13681 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    13741 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    13801 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    13861 gctgacggca ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc
    13921 cgtagcggct cggaataaat tttcgttctt acattccgtc tcatagtctt tgcgaggcgc
    13981 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14041 cagcaaccgc ttccggtctg cgctcgtcgt gcggtgagaa ctgttccagc tcggggtgat
    14101 tgtcggcata gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg
    14161 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
    14221 gtgcaagcag ccggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14281 caggctcaat gccgccagta catggacgca ggcgtgggaa ggagacagag tagcggcttt
    14341 gccagtaatg ctgttgcagc catagcaaat gttctgcaac catatagcag tcaacgcgcc
    14401 agctgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacggccca
    14461 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc
    14521 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagtt
    14581 gctttaactc ggcgtattcc gtctccgcca gcggctgtac ttccatctgt agtgaagaga
    14641 actgttcacg cagggcaggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    14701 gttcaccggt gactaaaagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc
    14761 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg gttgctcata gagaatccgc
    14821 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    14881 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    14941 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta
    15001 gcgcccgctc tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt
    15061 ccagttgtcg ccagcgatcg ctgaaggttt tcataccgat gcctccagaa atccggtcag
    15121 cgggctggtg gcatgagcaa aataactgcg gctgcccggt ccggactgag gtgccagcag
    15181 gcctgcttct actgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc
    15241 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct
    15301 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
    15361 cagcatggcg cgggtttcca gcccctgatt cgagccaatc ggcgcgccga gcggcatcac
    15421 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    15481 cagcacgaca aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc
    15541 gggcaacagc cagcgggcgt cagggtgaat ctccaatttt aaccagtttg tgcctaacgc
    15601 ttcacgagcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt
    15661 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    15721 caagtcgaca cgtttcatcg ccagcgtcac cagctggctg ccggaagcgc ggattgcctc
    15781 taccatcagt tgtgaagagg cgaatttccc ggtgccggta aacagatgtg aatcaaacgt
    15841 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagt aggatctggt
    15901 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
    15961 ccagagccgc gcccgcttgt cgttgaacga gttgctccag tagttcgtga acagtttgcc
    16021 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16081 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac
    16141 agtcggagtt ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc
    16201 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16261 tctggctcct ggttttctgg ccacaggcag cggtaacaac cctgccccca gggcggcgtc
    16321 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16381 gccacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    16441 acatcggctt gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    16501 gtcagttgaa tatcgggatt caactgtgtc agtcgctgat ggctgacctg cgatttcggg
    16561 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    16621 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt
    16681 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    16741 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    16801 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    16861 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    16921 tgccagactg ataccgccaa tcgccacggt gggataatcc gacagtcgct caacatgccg
    16981 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17041 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17101 atgggtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17161 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta
    17221 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17281 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17341 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
    17401 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    17461 ccgcctctgg cacggaagtt ctccgacata tccgctattc ccatttcaat agtttgcgtg
    17521 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
    17581 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    17641 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    17701 ttcgacatgg cgttatcgcg aatttgtgcg cccggatgcc ctttcgccag gtcagcggcg
    17761 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
    17821 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    17881 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    17941 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18001 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacatcata ttcccaggcg
    18061 atttttgtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18121 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18181 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18241 catttcgcca taatcgaacc gccgcgcgag acgataccgg tcaggcgttt cgcggtcatc
    18301 ggcacatagc gcagcagcac ccccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18361 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccattaacc
    18421 ttctccagcg cctggtagat cggcactgta ccaatcggca ccgggctgtt acgcaaaatc
    18481 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    18541 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    18601 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
    18661 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    18721 aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg
    18781 cgtaaaactt cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    18841 atttccggtg tgatgatgcc ctggcgggcg tagtgcagtt gtgtgacgcg gcgtcctgct
    18901 ttggcgcgtt ttggcgttaa tacgccgcta aaacgcagct cgtcgaggcc atcatctgcc
    18961 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19021 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga
    19081 tcgccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19141 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
    19201 acgccgggtt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
    19261 agggtgtcga taaaatgttg ggcctgggcg cgttgttcgc gacgggtcag ttttgttgca
    19321 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
    19381 acgatcgccg caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag
    19441 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
    19501 gaaattagcc ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca
    19561 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    19621 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    19681 atcatgatcg atagcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt
    19741 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    19801 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    19861 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    19921 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    19981 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    20041 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    20101 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    20161 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    20221 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    20281 ggaacctgtt tggttaatac aacagcagcg gcgttacgat atggggtcgg tacgtcaggt
    20341 cattgatctc gatgttgggc tgtttcaact ggccggacgt ggcgtacaac tggcggagtt
    20401 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccaa gcaaaaccga
    20461 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    20521 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    20581 cggtgtccat acggtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcggt
    20641 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa cacttgcgtt atgtgacttc
    20701 ccagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    20761 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    20821 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
    20881 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    20941 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    21001 cactcctgta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    21061 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gtgaagtgac
    21121 cttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    21181 cgtcccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    21241 ctcgccagtc acctgcaaag ctgacgtcga taaactgcca attccggacc cggaagatga
    21301 actggggtac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
    21361 gctgattggt ttttccggca gcccgtggac gctggcgacc tacatggtgg aaggcggcag
    21421 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    21481 tctactcgat aaactggcga aaagcgtcgc tttgtatctg aatgcgcaga ttaaagccgg
    21541 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    21601 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    21661 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    21721 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    21781 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    21841 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    21901 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    21961 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag cgattatgga
    22021 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
    22081 actcgataag gatccgccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
    22141 agaagtgacg cgagcggcga tggtgctgct gaaatacccc tcgcttgagc tggtggagta
    22201 taaagtggcc cgcatcgcta ccaccatgcc ttacattcca ggttttcttt ccttccgcga
    22261 atatcctgcg ctgctggcag cgtgggagat gctgtcacaa aagccggatt tagtgtttgt
    22321 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggtttgct
    22381 ggtggatgtg ccgaccattg gcgtagcgaa aaaacgacta tgcggtaaat tcgaaccgct
    22441 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
    22501 ctggcgcagc aaagcgcgct gtaacccgct atttatcgct actggtcatc gggtcagcgt
    22561 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
    22621 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
    22681 gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa
    22741 cccaattcat ctgcgtctgg aacgcctgga aagctggcag catgtcactt tcatggcttg
    22801 cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga
    22861 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagatgc
    22921 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    22981 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    23041 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    23101 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct
    23161 caaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    23221 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    23281 ggcggatgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    23341 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    23401 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    23461 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    23521 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    23581 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    23641 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    23701 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    23761 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    23821 accccttttg tctttctggc gtcgatcatt gatgctggct ggcgctctgt ttctcactgc
    23881 ttgtagtcac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    23941 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg cattttcttg ccgtgtttag
    24001 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    24061 gctcatcaat atcaatgttt acagcaaacc cccggtgaat attcgcgcgc gttttgacga
    24121 tcgcggtgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    24181 cgaccaaatc gatttatacc gttatcgggc tgatcagatc cgccagatta gcgatgcctt
    24241 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    24301 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcaatag cgcatatcga
    24361 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    24421 ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac
    24481 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc
    24541 atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcaatatc ccgtttcacc
    24601 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    24661 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    24721 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    24781 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    24841 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    24901 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    24961 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25021 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25081 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25141 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25201 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25261 attatggccg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25321 ttcgtgctct cgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25381 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    25441 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    25501 ccccgcagga aatgcagcag ttacatccgg gagatgaaga agtgtggcgg cggatcgata
    25561 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    25621 gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg
    25681 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    25741 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    25801 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    25861 cgctggggca tcttgcggca ggcgttgccc acgaaattcg taacccgctt tcctcgatta
    25921 aagggctggc aaaatacttt gccgagcgcg cgccagcagg gggagaagcg catcaactgg
    25981 cgcaggtgat ggcgaaagaa gctgaccgtt taaaccgcgt ggtaagcgag ctgctggaac
    26041 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26101 cattacagct ggtaagccag gatgcaaact gccgggagat ccagttacgc tttaccgcca
    26161 acgacacgtt gccggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26221 tttatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccagcg
    26281 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26341 agcttgaagc catcttcacc ccgtacttca ccaccaaagc cgaaggcacc ggactggggc
    26401 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagcctgg
    26461 agggaaaagg cgcaaggttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    26521 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    26581 tattttgcag gctttgctgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    26641 acaggcgctg gagcaggtgc gggagcgggt ttttgatctt gtgctttgcg atgtgcgaat
    26701 ggcggagatg gacggcattg ccacgctaaa agagatcaaa gcgttaaatc cggcgattcc
    26761 ggtactgatt atgaccgcat actctagcgt cgagacggcg gtagaggcgt tgaaaaccgg
    26821 ggcgctggat tatctcatca agccgctgga tttcgataac ctgcaggcga cgctggaaaa
    26881 ggcgctcgcg catacgcaca ttattgatgc tgaaacgcct gcggtgactg ccagccagtt
    26941 cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27001 gccatcggaa gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc
    27061 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27121 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27181 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgttgtttct
    27241 cgatgaaatt ggcgatatct cgccgatgat gctggtgcgt ctgctgcgtg cgattcagga
    27301 gcgcgaagtt cagtgtgtcg gtagcaacca gactatttcg gttgatgtcc ggctgattgc
    27361 cgcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    27421 ctatcgcctg aacgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    27481 tcctctgctg gctggccatt ttctgcagcg ctttgctgaa cgtaatcgca aggcggtaaa
    27541 aggctttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    27601 tgaactggaa aatgcggtgg aacgggcggt ggtgctgctg actggggaat atatttccga
    27661 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggcacaaa gcctggatat
    27721 tcagccgctg gtggaagtcg aaaaagaagt gattctggcg gcactggaga aaacgggcgg
    27781 caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctgt tggcaaaatt
    27841 gtcgcgttag ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca
    27901 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgtgctt ctgctacggt
    27961 atgacccagc gcggtgacgc acagtacgcg cccgccgctg gttaccacct gctcgtcatc
    28021 cgccagtttt gtgcccgcgt ggaacacttt gccgtcttcc acttcttcca gcggcaggcc
    28081 gtggatcacg tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28141 gagagaagca cgttcatccc actctgacgt tttctcgtct agtttgcctt cacaggccgc
    28201 caggcagagt tcaacaagat cggacttcat gcgcagcata atcggctggg tttctggatc
    28261 gccaaagcgg cagttaaatt cgataacctt cggattgccc tgtttgttga tcatcaggcc
    28321 cgcgtagaga aaaccggtgt aggtgttgcc ttccgacgcc atgcctttca cggttggcca
    28381 gatgatacgt tccatcgtgc gctgatgaac gtcatctgtc actaccggcg caggagagta
    28441 agcgcccatt ccgcccgtgt tcggcccggt atctttatcg cctacgcgtt tgtgatcctg
    28501 gctggtggcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    28561 gccgtcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaacg cgttgcccgc
    28621 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    28681 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    28741 cagcgcaggt tctacctcgg tgaagttctg atattccgcc gtaggaatgt tatggcgggc
    28801 caggaaatct ttagtaaacg ctttcgagcc ttccagctgt gcggcgcctg cggttgggcc
    28861 gaagattttc atcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    28921 cgggccgacg atggttagat ctaccttttc gttttgcgcg aaatccagca gcgccgggat
    28981 atcggtcacg ccgatagcga cgttttgcag cgtgggttcc agcgctgtgc ctgcgttacc
    29041 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29101 ctcgcgcccg ccgttaccaa tcactaatac tttcatctgt cgctccatta atggcggaag
    29161 tggcgcatat cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29221 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29281 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29341 aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    29401 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    29461 aacttcgcca ctttccagca gaacagcgca tcacgcagtt cctgttcggt cggctgacgt
    29521 ttggtgacga cgcgcagttc ttccgcaccg accatgccca gatcgcgatc ctgaaccagc
    29581 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    29641 gtcagaacgc gtacattctg tttggcggcg gtgattttca gggcttcttc gctggcggac
    29701 ggcgcaataa tcacttcaac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    29761 agctcgcggt taaaggcaat gatgccgccg aatgcggagg tcgggtcggt tttgtacgcg
    29821 cgatcgtaag catcaagaat tgaattgccg atagccacgc cgcaagggtt ggcgtgcttc
    29881 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    29941 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30001 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30061 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    30121 ttgctttcac cgtgataagc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30181 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30241 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30301 attgcgacat ctttatggtt cttggcggcg gagcgcacca tcgttgggcc gccgatatcg
    30361 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    30421 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    30481 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    30541 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    30601 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    30661 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    30721 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    30781 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    30841 tgtaacgaaa acgtttgcgc aacgctcgcg aattttttta attcgatggc gatcacgatt
    30901 ttgcccccga ttagcgtggg agaaatagag aaataacgct gatgattgtg gataactctg
    30961 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    31021 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    31081 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaat
//
LOCUS       DAZFTN010000057        29430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFTN010000057 DAZFTN010000000
VERSION     DAZFTN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHKV2_002752"
     CDS             complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHKV2_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO6940615.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHKV2_002753"
     CDS             complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHKV2_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6940616.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1680..3413
                     /gene="argS"
                     /locus_tag="ACHKV2_002754"
     CDS             1680..3413
                     /gene="argS"
                     /locus_tag="ACHKV2_002754"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO6940617.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3455..3499)
                     /locus_tag="ACHKV2_002755"
     CDS             complement(3455..3499)
                     /locus_tag="ACHKV2_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940618.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3590..4078
                     /gene="yecT"
                     /locus_tag="ACHKV2_002756"
     CDS             3590..4078
                     /gene="yecT"
                     /locus_tag="ACHKV2_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO6940619.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHKV2_002757"
     CDS             complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHKV2_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO6940620.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHKV2_002758"
     CDS             complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHKV2_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6940621.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHKV2_002759"
     CDS             complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHKV2_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO6940622.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHKV2_002760"
     CDS             complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHKV2_002760"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO6940623.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHKV2_002761"
     CDS             complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHKV2_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO6940624.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHKV2_002762"
     CDS             complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHKV2_002762"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO6940625.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHKV2_002763"
     CDS             complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHKV2_002763"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO6940626.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHKV2_002764"
     CDS             complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHKV2_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO6940627.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHKV2_002765"
     CDS             complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHKV2_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO6940628.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="ACHKV2_002766"
     CDS             complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="ACHKV2_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO6940629.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="ACHKV2_002767"
     CDS             complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="ACHKV2_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO6940630.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPCEADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16945..17871)
                     /gene="motB"
                     /locus_tag="ACHKV2_002768"
     CDS             complement(16945..17871)
                     /gene="motB"
                     /locus_tag="ACHKV2_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO6940631.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17868..18755)
                     /gene="motA"
                     /locus_tag="ACHKV2_002769"
     CDS             complement(17868..18755)
                     /gene="motA"
                     /locus_tag="ACHKV2_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6940632.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="ACHKV2_002770"
     CDS             complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="ACHKV2_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO6940633.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="ACHKV2_002771"
     CDS             complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="ACHKV2_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO6940634.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20593..21021
                     /gene="uspC"
                     /locus_tag="ACHKV2_002772"
     CDS             20593..21021
                     /gene="uspC"
                     /locus_tag="ACHKV2_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO6940635.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21028..22452)
                     /gene="otsA"
                     /locus_tag="ACHKV2_002773"
     CDS             complement(21028..22452)
                     /gene="otsA"
                     /locus_tag="ACHKV2_002773"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO6940636.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(22427..23227)
                     /gene="otsB"
                     /locus_tag="ACHKV2_002774"
     CDS             complement(22427..23227)
                     /gene="otsB"
                     /locus_tag="ACHKV2_002774"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO6940637.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23394..24380)
                     /gene="araH"
                     /locus_tag="ACHKV2_002775"
     CDS             complement(23394..24380)
                     /gene="araH"
                     /locus_tag="ACHKV2_002775"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO6940638.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24395..25909)
                     /gene="araG"
                     /locus_tag="ACHKV2_002776"
     CDS             complement(24395..25909)
                     /gene="araG"
                     /locus_tag="ACHKV2_002776"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO6940639.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25979..26968)
                     /gene="araF"
                     /locus_tag="ACHKV2_002777"
     CDS             complement(25979..26968)
                     /gene="araF"
                     /locus_tag="ACHKV2_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO6940640.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27765..28268
                     /gene="ftnB"
                     /locus_tag="ACHKV2_002778"
     CDS             27765..28268
                     /gene="ftnB"
                     /locus_tag="ACHKV2_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO6940641.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28348..28599)
                     /gene="yecJ"
                     /locus_tag="ACHKV2_002779"
     CDS             complement(28348..28599)
                     /gene="yecJ"
                     /locus_tag="ACHKV2_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO6940642.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28714..28800)
                     /gene="azuC"
                     /locus_tag="ACHKV2_002780"
     CDS             complement(28714..28800)
                     /gene="azuC"
                     /locus_tag="ACHKV2_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO6940643.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            29062..29385
                     /gene="yecR"
                     /locus_tag="ACHKV2_002781"
     CDS             29062..29385
                     /gene="yecR"
                     /locus_tag="ACHKV2_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO6940644.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         7273 a         7873 c         6996 g         7288 t
ORIGIN      
        1 cactgtcacc cgctcaacag atcgcggtaa tcgttcattt tttatactat tcagggataa
       61 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
      121 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
      181 accgccgccc cgtctacgac ataacgcgaa tactcgtccg cgtgtgcatc tgatgacatg
      241 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
      301 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg
      361 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
      421 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
      481 ttgagtgtat ttagagggtt agcgcacata tcaaaggcgc gatgaaaagt cactgccagc
      541 ggaccggcag cagccattat tttttccatt cgtgacatat cgacattacc gtcaacatcg
      601 agcacgcccg tcacgagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
      661 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
      721 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cacccaacga cggcgttaag
      781 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
      841 agcgcgcatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
      901 cgccgactgc tcgctggcaa caatctcttc aatggaccac ggatgaaatt taatggtcgt
      961 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgt tcatgctcac cttttggcga
     1021 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     1081 acgggcattc agcgtttctg gatcacccgg caaaacgatt tcaatatgtt cccaaccttc
     1141 gtgcgggtaa cgtttttccc taggccacgg tagctccaca atagaaaact gccagtgtgc
     1201 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc
     1261 tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgctcagcag tggcgttttg
     1321 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggcg
     1381 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     1441 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     1501 ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     1561 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     1621 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     1681 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     1741 ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt tcagttcggc gactatcagg
     1801 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     1861 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     1921 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     1981 cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt gactactctg
     2041 cgccaaacgt ggcgaaagag atgcacgtcg gtcacctgcg ctctaccatt attggtgacg
     2101 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     2161 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     2221 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
     2281 acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
     2341 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
     2401 tcacttacga tcgtctcaac gtgacgctga cccatgatga cgtgatgggc gaaagcctct
     2461 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
     2521 gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg
     2581 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
     2641 cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
     2701 agcatcaaca cctgatgcag gcatgggcta tcgtccgtaa agcaggctat gtaccggaat
     2761 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
     2821 aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct ggatgaagcc ctggaacgtg
     2881 cacgccgtct ggtggcagaa aagaacccgg atatgccagc cgacgagctg gaaaaactgg
     2941 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
     3001 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
     3061 agtatgcata cacgcgtgta ttgtccgtgt tccgtaaagc agaaattgac gaagagcaac
     3121 tggctgcagc tccggttatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     3181 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg
     3241 cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     3301 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     3361 cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg taatcgattt
     3421 ttcgtgagag tgaagcctga tcaggtttag ccgatcaggc ttttttatta ccatctaaat
     3481 gtattctgaa aataggcatg ccattgtttt ctcgctgttg agtaagaggc cagaagcgta
     3541 atatccagct ccagggaaac ggtaacggtt caatttaagg aataccgcag tgtttaaatt
     3601 tcttgtatta acattaggca ttatctcttg ccaggtttac gcagaagata cgcttatagt
     3661 aaatgatcat gacatttcag ccatcaaaga ttgttggcaa aaaaattcag gtgatgatac
     3721 tgacattaac gtgatcaaat catgtctgcg acaagaatac aatctcgtcg atgcgcaatt
     3781 aaataaagct tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgcaaa
     3841 aaaacctgat actgaacaac ttaacttgct taaaaaatca caacgagcct ggctggattt
     3901 tagggacaaa gaatgtgaat taatcctttc aaatgaggac gttcaggatt taagtaaccc
     3961 ttattctgaa tcagaatggc tatcatgtat gatcatacag accaatacgc gaactcgcca
     4021 gttgcagcta taccgtaact ctgaagattt ttatccaagc cctttgacaa gaggataatt
     4081 cacatatttt tggcatgttt tgttgcaagc tattcctgat aaatgattgc aacaaaacat
     4141 ccagcctttt tcactgagtt attaaacata ctcgcgagcg cgtaattttt ttgtccttca
     4201 gcgataattc acaatcactt cattacgttg tactttcagc gacggaatta accgcccacc
     4261 gccaggcact tcccagataa agcgcaacgg ttctgctgcc gctataccgg aaaaggccac
     4321 ggtggttccg ctctgcccat ctaattcgac acaacgagat tgcgaacaca agcgaacccg
     4381 cagtcctgca ggtgtcgggc cgataagctg ataccgccac gccaccagcg tcatcaatcc
     4441 tgaagcaggt tgtcgcgtag aaagaggtgc agacgacatc gactcaccgc gatgatttag
     4501 cgtaattccg acactacttg cctgccacat cccctccccg gcggcttgca ccagcagcgg
     4561 aaacaataat attgctaata aggttctcat catttaccgc caattgtcgc cgtcatgcgg
     4621 atatgtcggt tatcagacag ttccagattc gacagcacca ctaactgcgg caagctgcgg
     4681 cgcagaaagc gagacaataa tggtcgcagc gcgtggttca ccaataaaac tggcggcgca
     4741 cccaacatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
     4801 cctggctcca gtccgccccc gccctgcaac gcctgtagca acaaacgttc cagcggtgta
     4861 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcccgt
     4921 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cgctttggat gggcgcatgt
     4981 tccgccagcg tttcgagaat agtgcgcata tcgcgaatcg gtactttttc atcgaggaga
     5041 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc cagggacgag atcttccgtc
     5101 agctttggca tctcctgggc gacgcgatcc agcagctgtt gcgcctcctg acgaccaaac
     5161 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
     5221 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
     5281 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
     5341 ccagggttaa tagccagcca gcgccccgga taagcatcac cactgcctat ctccacgcct
     5401 ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat gtgcaccact
     5461 ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
     5521 aactcaccat cctgctggaa atcgaccatc gggatcagtc gataacccac ttccattccc
     5581 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggcatt attctctgcc
     5641 atttttaccg gttttggttc ggcaggcgct ttttgttcgc gtccacggat ccaccaggcc
     5701 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
     5761 ccgagtaaac cgagcacggc agcgcttaac aacataacgc ttgggttact gaaaagctga
     5821 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacaat gacccccgcg
     5881 gcggtagaaa tcaccagagc cgggatttgt gccaccagac cgtcaccaat ggtcaagagc
     5941 gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc gaccagcaac
     6001 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
     6061 ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtgacttc ggagcggcgt
     6121 tttttcgcct catcttcacc aatcaatccg gcgttaaggt cggcgtcaat cgccatctgc
     6181 ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg cccggcacct
     6241 ttggtaatga ccataaagtt gatgatcacg agaatgacaa acaccacgat accgatagcg
     6301 aaattgccac caacgaggaa gtgaccgaac gcttcaacca ccttccctgc cgccgctgcg
     6361 ccggtatgcc cttccattaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
     6421 agcgtggtaa acaacaaaat ggtcggaaac gcagcaaact caagcgtgcg ctgggtaaac
     6481 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa caggtcgagt
     6541 atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa aatcggtccg
     6601 gcaaggatct gccattgtgt cgatttcagg tttgtgggca ggcgcagcat cgcggccaga
     6661 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
     6721 aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     6781 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccacatg
     6841 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     6901 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     6961 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     7021 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgacg
     7081 gatccgccct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     7141 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     7201 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     7261 acccatggcg gtaatcggag actcggccat taagcgcatc atatgcggcc agtgatgcca
     7321 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgctt taagcaactc
     7381 cgcgccagtc tgtgccgaga acatccgttt tatgcccggt agcgggttga gtttggaaaa
     7441 cttcggctgc aaggattttc cgctaaatac cagccccccc agcataaccg gagaaatgag
     7501 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     7561 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     7621 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     7681 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     7741 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     7801 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     7861 cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ctgccacaca
     7921 tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc gcctgatatg
     7981 acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc
     8041 cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag
     8101 caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac
     8161 catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc
     8221 ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct
     8281 ggtatgcgcg ggtacatctg ccagaaattt ccgcgtatct gttaccagtt cacgggcgtc
     8341 ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga
     8401 tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc
     8461 agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc
     8521 cgccgcttcg gcaatggcct gatccagtcc cagttcccgc aaactgtcgc gcagcatacg
     8581 cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat
     8641 tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt
     8701 tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg
     8761 ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg
     8821 acatcgcgcc atccgcacga atcgttttca gcaattccag gccatccatg ttgggcatgt
     8881 tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc
     8941 catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc
     9001 gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac
     9061 actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct
     9121 tacctggtta agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat
     9181 gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgccccag cctgacgcat
     9241 cgccaacatt cccgccgcgc cgtcgttgcc cataccggtc aggatcaccc caaccgcatt
     9301 acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc gatgacggtt
     9361 aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg ccactacgcg ccagctccat
     9421 atgccgatcg cccggcgcaa tataggcatg ccccggcaaa acacgttccc cgtcttcggc
     9481 ttctttaacc ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc
     9541 gggcggcata tgctgggtaa ttaacagtgc cgggctggaa agcggcaacg gttgcagtac
     9601 gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt tttcagaact
     9661 caacaacggc cctgccttca gcgttgtcgg tgccgacaat ggcttatgtg ctgcaaggct
     9721 cgcctttgct gctgtacgca ccttttcagc aatcatttcg ctgtacgcca gcattccttc
     9781 gcgaataccc agttgcggtt tggtgacaaa atctattgcc cccagctcca gcgcgcgcag
     9841 cgtgacttct gatcctttgc cggtcaggga agaaaccatc acaacgggca ttggacgcaa
     9901 acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt
     9961 cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg accagcggat caggcgcggt
    10021 cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag
    10081 tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca
    10141 tacaccgttt gaccacgcag cgtgaagcgg cgctcgaggt ggctaaagtt ttcagagtga
    10201 cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa aatctcctgc
    10261 tgggtagttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc
    10321 ggcacggtgt actgtttcgc cagtagattc agcgaggcaa aatcaacata gttcgccagc
    10381 tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc
    10441 agttgctgcg gcgtcaggtt tttcaactct tcatggcgat agataccgct tctggccttt
    10501 tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg cgcggtgccc
    10561 aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga agccgccgcg
    10621 ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag agggaaatga
    10681 tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac
    10741 tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc cgtcagtccc
    10801 agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga
    10861 acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc
    10921 gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc
    10981 atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca
    11041 tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg
    11101 gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga ggcgttctgc
    11161 tgtgtcacct gatccatctg gctgacagcc tgcgcaacct gctcaatccc ccgccgttgt
    11221 tcttccgagg ccgaagcaat ttctcccata atgtcgttca cgcgggtcac cgaactgaca
    11281 atatcggtca tggtcgcggc ggcgttattc accagtttcg aaccctgctg gacacgattg
    11341 actgactctt cgatcagccc tttgatctct tttgccgcct gggcgcttcg gctggcaaga
    11401 ttgcgcactt ctcctgccac taccgcaaaa ccacgcccct gctctccggc gcgagccgct
    11461 tctactgccg cattcagggc cagaatattg gtctggaaag caattccgtc gataacgctg
    11521 ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg agtcatggta
    11581 ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgcaag ttccgacgcc
    11641 tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat actggcggcc
    11701 gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt attgcctgcg
    11761 acaatctccg cgataccaat gtgcatctcc tggcttccct tacgcacatc acttaccgtc
    11821 ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggcggtgat ctcattacga
    11881 ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa atggctaccg
    11941 ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact gctgatgtag
    12001 atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata gttacgctgg
    12061 ctttgcgcac tggcggcttc cagcacatgg atgatctcca gctgccaggc ctcaaagtta
    12121 acgtcaaacg cattctgtga tccctgcacc ggcgcagtga ggaaatccga aagttgatta
    12181 ctttcaagcc aggtcgcctg atgttcgaga tcgttgtgcc agcgggcaaa acttttctcc
    12241 gtctcttttt gcaatgccct gacgtgctcg ttgcccgcag tcatcgccat aaaactttta
    12301 aacagcgtgg tggattgcgt cagactggca cgcgccgtcg tcatcaacgt tttaatgtca
    12361 tccgccggat agctaagcgc tgtcagagtg cctgctttgt tcagcgtggt actggcctgt
    12421 aacattaccg cccgagtttg cgctaatgcc gcacgttgct gattgctctg ctcgacctga
    12481 ttcagtcgtt gcaaatcgtc gcgaaatgcc caaaaagaca tgccgttact gccaatctgc
    12541 aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat acgattaaac
    12601 atcaacgctc cccatcaggc ggcaatgacc gcgttagtaa atactcgtca aaatgtttcc
    12661 cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg ctcactggca
    12721 ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg aaacgcggaa
    12781 accgcttgcg ttaaacgact agcctgttct tccagcgcag ctgcagcggc agctgattcc
    12841 tgcaccagcg atgcgttctg ttgcgtgacg tgatccattt ccgaaaccgc caatgcgact
    12901 tgatcaatgc cacggctctg ttcatccgat gccgatgcaa tctcgcccat aatgtcagtc
    12961 acgcgagtga cagcatcgac gatattgttc attgtttccc cggcgctttc gaccagcacc
    13021 gaaccggtat caacgcgcga gacggagtct tcaatgagag ctttgatctc ttttgccgcc
    13081 tgggcgctac gactggcaag attacgcact tcgcccgcca ccacggcaaa accacggccc
    13141 tgttcacccg ctcgcgcggc ttcaacagcg gcattcagcg cgaggatatt agtctggaag
    13201 gcaataccgt cgattacgct gataatgtcg gcgattttct tcgaactatc ggcgatctca
    13261 tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt gtcggaggca
    13321 ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac tgtcgcggtg
    13381 agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc cgtacgggag
    13441 gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc atctgaacct
    13501 tcgcggacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac gctctgcgcc
    13561 aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt accaccggcg
    13621 atttcgcgaa tgtgagcaat aatttttgcc agcggtgtaa gcaacatacg gcgaatgcca
    13681 taccacgcca ccagcagaat caataccacc accagcgcga taaccgccag ttgccactgg
    13741 gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt ttcactgctg
    13801 agggcgtact gagcaaacgc ttcgcccatt gcattttgca ttccctgggt tggctgagcg
    13861 aaataagctc cagtattgcc ataatcaaga taatcaatca gttcagttaa cgctgtgtaa
    13921 tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg taacggtgcc
    13981 atgcttttga attttttata atgagtcgcg gcctgggcca atgttttcct ggcgctatcg
    14041 agcaattcaa ctttggcatt actttgttga ttggaggaat ccatcatcat tcgcaccgct
    14101 gaacgactca ggttaatgcg cgtttgcagc attaaatccc aggttgacgt cagctcgccc
    14161 tgctgttctc gtaattgatt ggaaaccaca aagctcttct ggctatggtg aagggaagaa
    14221 aaaaacagac tgccggaaat aagctgtaac agtgcgaata cccccagcac cattaccaac
    14281 agcgtgacta cgcggatacg gttaatcata aggcaccttc ctgaaaacaa gttgatctcg
    14341 ttatcggcaa ggaaggggga aactttattg ctgatgccac ccgccgcgaa attgaaataa
    14401 aacccgatgc gcagatcatc gggttcattt caattgagga aatcaggaga attacgccac
    14461 ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
    14521 caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag
    14581 cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
    14641 aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt
    14701 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
    14761 aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
    14821 tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc
    14881 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
    14941 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
    15001 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
    15061 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
    15121 actacaacct gatgttgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca
    15181 ctttgtaaga tcaccacaat tccctgggta gcttcggttt tcgcgcccgc gacgttgaac
    15241 actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac ccgctcgccg
    15301 ccggccagtg gatgcagatc ggcttcacag ggttgcagtg attccataac agcattcagc
    15361 ggcagaatga atacttcatc cgcaacgcgt acggacatgc cgtcgaggat ggccagcgtc
    15421 agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat ttcaacatga
    15481 ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg cccggagacg
    15541 tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcatcgc gacttcgtcg
    15601 tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag aattcgctca
    15661 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
    15721 gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt
    15781 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttct
    15841 atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac ctgcttgccg
    15901 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
    15961 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
    16021 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
    16081 atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc
    16141 gctacacgga tgctggtgga ttcactgcta cgcgtcgttt tttcccgctc cacgcgaccg
    16201 gttggcgctt gttcggctgc cagtttaagc actggtgggg tggatatttt tggcgagact
    16261 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
    16321 tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc tttcaccacg
    16381 tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gctggccttc
    16441 aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tctgctcatc ttgcggttca
    16501 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat
    16561 gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac
    16621 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
    16681 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
    16741 tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg
    16801 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
    16861 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
    16921 tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact
    16981 gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc
    17041 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg
    17101 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgaccac
    17161 acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg atgcattggc
    17221 ccgatcggca gaaagctccc agttgctgta acctttctca ccactggcgt aggggaaatc
    17281 atcggtatga cccgaaaggc taatacggtt gggaatacca ttcagaacgg gcgcgatggc
    17341 acgcagaatg tcgcgcatat agggttcgac gtcggcactg ccagttctaa acatcgggcg
    17401 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
    17461 tggacgtaac gcccgcagtt tcggatcgga ctctatcaat tggtcgagat cgccacgcaa
    17521 tttccgcaat cgactttgct ccatgcgttt tttcaactct tcgatgttcg gctgcttatt
    17581 cacttccccc tggctttggg tgtaatcatc gccaccgccg ggaattgggc tttcactatt
    17641 agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt actccgcaat
    17701 ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat
    17761 catcgcggtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
    17821 tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
    17881 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac
    17941 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    18001 taaccgttca gattagaaag cagtgtgact ttgacgcact gcatcatttt gctggtttcg
    18061 gcgcttttct gacgtaaaac actcgctaat ggggaaataa atccgtaagc caataaaatg
    18121 ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc ggcaggacga
    18181 tcggctgaac ctaacgcgtg aacgaccccc attacagccg cgacaatacc aaacgccgga
    18241 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    18301 tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    18361 aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgatgat gcgtgggtag
    18421 ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc
    18481 atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat atacattgct
    18541 ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc tttaatcgct
    18601 tttccattat tgccgacaat aaacgaccca atccctgcac cggcaataat caccagttca
    18661 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    18721 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    18781 tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa agcagcggta
    18841 cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc ctgtactctc
    18901 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    18961 actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg ctggtgagcg
    19021 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    19081 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gcggtccttc ttctgcttgc
    19141 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaaa
    19201 ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac gttttgttcc
    19261 caagtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg gcttccgcgc
    19321 agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct ttccagcatc
    19381 tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg cgcttcctga
    19441 acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct tcaggctgat
    19501 taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc tggagatcgt
    19561 caacgcggga atcttgcgtc aacagagtaa tcgtctggtg gctgtcaaaa cggaagtgac
    19621 aaaccagctg attggtttct gccagcttaa ccatttgcgg aagagtcagt gccgctaatg
    19681 ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg tcctgaacaa
    19741 tcagacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt ttcagcaact
    19801 cggaggtatg cattattccc acccagaata accaacttta tttttatgcg gtttcaccgc
    19861 accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa tagttgcgat
    19921 aagctgcaat aagcagagcc acctttttgg tttaataagt ccttacaaat agaagggggt
    19981 ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca ctacacgcac
    20041 atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca caaaacactc
    20101 aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc aatctgttaa
    20161 aaagtttttc gctacgctag caagcaaaaa tgaaacagga ataatcgaaa tgggatgttg
    20221 cgcacagtta aaataactca ccgtaaataa tcatctgcta taaataatca ctttcatgca
    20281 ataccagata agctattttt aaacagacac ttaccgcaca acaaactaat taaatacaaa
    20341 gaaaggcaac aatcaactta atgttaataa aatgttttgc atatattaac cacttgttta
    20401 atgcggggaa tcagtacatt attttcttat aattacagtt gaaatcatat gatcaccgag
    20461 agtgattgat taattgtctt tactaataat cggactaata tttacctgtt tgactgagtt
    20521 gggattgcgt cgtttctcca ttaggagtaa agctttaatg tcacctgaag ttcacagaat
    20581 aaggaacagg ttatgagcta tagcaatatt cttgtcgctg ttgcggttac accggaaagt
    20641 cagcaactgc tggcaaaagc ggtatctatc gccaggccag taaagggaca catcagttta
    20701 attactctcg cttccgaccc ggaaatgtac aatcaattag ctgcgccgat gcttgaagat
    20761 ttacgcagcg tgatgcagga agaaacgcaa agctttcttg ataagttaat tcaggatgcg
    20821 gggtatcccg ttgacaagac gtttattgcc tacggtgagt taagcgaaca tattctggaa
    20881 gtttgtcgca aatatcattt cgatttggtg atttgcggta atcacaatca cagtttcttt
    20941 tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa gtgaggtcga tgtgctgtta
    21001 gttccactta cgggagatta accgctccta cacaagctta ggaaaggtag caactttatc
    21061 gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt aggtcgctaa tgaagcactc
    21121 ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc atttctgcat gacgggaaat
    21181 acgttccgcc agcgacatag tcaatgcacg atccagcgca gctgcaactt cgtcacgatc
    21241 gtagggatta acaattaacg ccgacgttaa ctcgtttgcc gctcccgcaa attgcgaaag
    21301 aacaagaacg cccgggttgg ctgggtcctg agcggcaaca tactcttttg ctaccaggtt
    21361 catcccgtca cgcagtggcg tcactaagcc cacgtcagag tagcggaata ttttcatcag
    21421 taatttacgg tcaaaatgct gattcaaata ataaagcggc gtccagccta attgcccgta
    21481 tttaccatta attcgtccgg cttcattttc gagctgatga cgaatatcct gataggcttg
    21541 cacatcacca cgcgacgttg gtgcaatctg ggtataacga attttaccat gatgctgcgg
    21601 atatttttcc agcaacgctt cataggcgag aaaacgctct ggcaaacctt tggaataatc
    21661 cagccgttcg acagaaaaga tattttgtac gtttttcagt tccgctttaa gttgcgccag
    21721 ttttggcggc agtggcccgg cagcctgttt ggctatttct tttggctcaa tgccaatcgg
    21781 gtaaacttct gttcgaaacg ctttgcccca ggctgtatgg ctttttgcgc tacgtgtcgt
    21841 gacgcgggtc aggttagaaa ggcaatccag gaacgccaga cgatcgtttt ctgtctggaa
    21901 acccagcaaa tcataatcac aaagctgttc aagcaaggtg tcatatgtcg gcagcgcgtt
    21961 gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa ccaatgcgat tattcactcc
    22021 ccgtttgcgt aattcatgcg caaatggcaa caggtgataa tcgtggatcc agataatgtc
    22081 atcgtcttgc aacagcggca gtaatttatc tgccagcaac gcatttacgc gtagatagcc
    22141 gtcccaggca ggacgctgaa attgcaccag atcgaggcga taatgaaaag caggccagag
    22201 aacggcattg gagaattggt tgtagtattc gtcaaggtcc tgttcgctga ggttaaaaga
    22261 ggcccacgta atgttaccct ttttcacctt ttttagcggc tgatcctcat tccctgtttc
    22321 accactccag ccaaaccaca gtccgcctgc ggctttcagt gcccccagta tgccaacggc
    22381 aaggccaccg gcactggcgg cgtgctcatc tggtggtgca atccggttag atactacgac
    22441 taaacgactc atagtcatca ctcctgttat tttctctttt ttgttgtaat gcggtggtta
    22501 tcatttcaag ccagctccag acatccggta cgcccgccag tcgccatgat gcctgagttg
    22561 cgcctgtgcc aatttttact gacattccgc ccagtcggtt aacgactgcg aagccagatt
    22621 catcggttaa atcatcgccc agaaatacgg gcgttcgccc gataaaggga gcttcctgca
    22681 taaaggcagc aatcgcctca cctttactgg tacctctcgg tttgatctcg acaacacact
    22741 ttccctgctg taacgccatt tgtggccaga tctgagtaat acgttgcgct aatgtcatta
    22801 atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc aaaagccatc cctttcgtct
    22861 ccagctccgc accgggatac tgggcgatga ccgtatgcag ttgcacgtta atatcacgtg
    22921 caatcgcatc cggcagatga acgatatgcg ttttaccatt gatgtcacgg cgctccgccc
    22981 catgcacgcc cgctaacggg aagcgataag gttttgccag tgcgtccagc tccaccattg
    23041 agcgccctga tatcaatgcc aatgcaccat cacttgcggt tgccagtagc tgaagtcctt
    23101 gcagaatatt gtcaggtacg acgacctgat cgggatgcgg tttgatttcc gccagcgttc
    23161 catcgagatc aaaaaaccag gcatatttcg cggatagttc aggggtttcg gttaacggtt
    23221 ctgtcacccg gttctcctcc ttctcttttc ttattgtgtt gcgcagaacg cgctttacag
    23281 acatgtaagt atagacagtg tgacgggggt cgccatttgc aacgaagaac agccaaccgt
    23341 tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt gtggaaaaaa gcatcagaca
    23401 gtgcgtttcg ctttttgctt gtaacggtcg aagatcaccg ctgccagcag gattaagccg
    23461 cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt tcatggcgtt ttccacggtg
    23521 cctaaaatta agatacccgc caccacatat gagatttttc cgatgccacc tttcagagaa
    23581 acgccaccta aaacgcaggc ggagataaca ataagctcat aaccaatcga cgtcattggc
    23641 tgcccactgg tcatacgtga agccagaata attccggcta tcgcagatac caggccagag
    23701 agaacaaaga taataatttt ggtgcgagca accggtacac ccgccagacg cgcagcctct
    23761 tcgttcccgc caatcgccag ggtattacga ccaaaggtag ttttattcag cagcaaacca
    23821 aagataatca gacacgcgac ggtgagccag attggcgcag gcagaccgaa ccagttggcg
    23881 taaccaaggg caaagaagct ttcatcttcg ataccgaccg ctttaccgtc ggaaatgatg
    23941 tacgccagac ctcgaacaat ctgcatcgtt gccagtgtcg tgatcagagc atttattttc
    24001 agtttggcga taacaaagcc attgaccagg ccacagagaa tgccaagcag caaccccgct
    24061 gccacgccaa tccacaggct ttcagtcaga ttgataacca ccgccgtggt gacacccgca
    24121 caggcaatta cggaggcgac agaaaggtca aagtcaccgg aagcaaggca gaacaacatg
    24181 ccacacgcca ccatccccga catggaaatt gccaggccca accctttcat attaataaag
    24241 gtggcaaagt tcgggacaaa aatggcacag gcgataaaaa gcaccgcaaa caccaccagc
    24301 atgccgtact gatcccagat acgcccgaag ctgaatgacg acttaggtgc gccagaaccc
    24361 gatgtagaaa cagaagacat catactctcc ttactcaggc aactgcctgg ctgactttag
    24421 gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc gtgtaacaat tcaccggcga
    24481 tttcaccttc gcgcatcacc acaatccggt cggcaacgcc gaggacttca ggtaagtcgc
    24541 tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc ataaatcacg ttatatattt
    24601 cgtgcttagc accaacatca atgccgcgcg taggttcatc cagcaaaatg accttcatct
    24661 cttccgataa ccagcggccc agaatggctt tttgctgatt tccacctgag agattcataa
    24721 tcagttgctc agcgcccggc gttttgatgt tgagcgaacg aatgtggtga tcggcattgt
    24781 tttcttccca accgttgttg attacacaac cgccgagcac atgtttacgt ctggcactga
    24841 tgttgatatt gtcgcgaacg gagtgcacag gaataatgcc ttccgctttg cgatcttccg
    24901 ggcagagcat catgcctgcg gcaatggcgt ggctcggttt acgaatatcg atcggctgtt
    24961 ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc aaacatgcct ttcattaatt
    25021 cgctacgccc cgcccctacc agaccaaaca gcccaacaat ttcaccactg cgaaccgcca
    25081 gacttattgg cgtacgcacg cctggtgctt tcacagcatc aagacgcagg cgctcctcgc
    25141 cataactacg cggttgccag ccgtagatat cgccaatgtc gcgcccgacc atcgcctgca
    25201 ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt tttgacataa cgtccatctt
    25261 taaagacagt gatggcatcg ctgagggcaa atatttcttc catacggtga gaaacgtata
    25321 agatcacccg cccctctttt cgcagttcac gaataacgcg gaaaagattg tcgatttcgc
    25381 gggcagaaag ggagctggtt ggctcatcaa aggcgataat tttagcgtta cgcgccagcg
    25441 ccttggcgat ttcaaccatc tgccactgac caatggagag atatttcagc ggcgtgtccg
    25501 ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc ctcataattc agcaatgagc
    25561 gattcacaat gccgccttta tgcggcagct ggccgagata gatgttttcc gcgacggtca
    25621 tttccggcac gagatgcagt tcctggtaaa taatcgccac acccgcgtta agtgctgcgg
    25681 tcgtgtcgga aaaggacatt tcctgcccat taatcactac agaacccgtg gttggcgcat
    25741 agttgccgct gaggattttt aagagagttg attttcctgc gccattttca cccatcaacg
    25801 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
    25861 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
    25921 gaatcactgg tcgttcacac cccctctgca tcatgcaaag gggaattttt ccggcaaatt
    25981 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagca
    26041 ccacgtcggt aacttcggtg aatttggtcg gttcaacacc ttttgctacc cagttgtaaa
    26101 gcatttcgct ggacttataa ccatgtacgt ccgggcttgg caacagggaa ccgtagaagc
    26161 cggttgcctg tgccttagac agttcgctca ccgcatccac accgttaata ccgataccga
    26221 tgatatcggc cgctttaaag ccctgacctt ccgtcgcgcg tacgccgccc agcacggtgc
    26281 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
    26341 tggcagcgtc aaatgccccg gggatgtcgt tagatttggt aggtacctga taaatttgtt
    26401 tttccgggaa tcctgcagct ttcagcgcat ccatagaacc cgtagtacgg cgacgagccg
    26461 tatccagttc gttggaggta atcgccatca ccgcgctttc tttgacatcc cagccacgtt
    26521 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttggtc gccgccatca
    26581 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccacgg
    26641 caatgacttt catatcgtag ccacgcgctt tcgccacgat ggcagagccg agtttcgggt
    26701 ccggagtaca aataacgaaa ccttttgcgc cactggcggc caggctatcg atcgcgttca
    26761 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
    26821 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagga
    26881 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
    26941 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgca
    27001 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttaacta
    27061 aaagataagt gactgtgttg acatagtttt agcgagaaat taattctcca taggagagca
    27121 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
    27181 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
    27241 tggaaatcct tgttgtcttt ggtacccatg cgggatgtct tctttttcac cagtcaatag
    27301 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accgcaactc aaagatgtgg
    27361 aaaatgcacg tcattcattt cgtcattaat tatcactgtg ctcattaatt aacagaacac
    27421 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaataat cacttcatca
    27481 tgaattcaaa ttcattgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
    27541 ttgcagttac attgatttca tcaatgaaat gtaaaaatat ataaacttga tgatttaagc
    27601 attttcttat acccgttcag acgttattct tatttcagat catcgtcaga attgactcca
    27661 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
    27721 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
    27781 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
    27841 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
    27901 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
    27961 ctacccccat cgttaaagcc attgatgttc ccggtgaaaa actgaactct ctggaagaac
    28021 tgttccaaaa aacgatggaa gaatacgagc aacgttctag tacgttggca cagttagccg
    28081 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
    28141 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
    28201 ttgcgggtat gtgcccagtg cagaccgacc aacatgttct gaatgtcgtg tcgcatcagc
    28261 tgcattgatc atacatcgac gctaatgcat tgcaccgatg aagtttttga gaaaccgctg
    28321 cctcatcttt tttgaagcag cggtttttta atgggattcg ccttgtgggg taaacttgag
    28381 ctcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
    28441 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
    28501 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag caaccacatc
    28561 agaaaccagt tcctgatcgg cattcagcgt ctgggacatc gtaaactcct gatagcattt
    28621 tgaaagccgt tatagtagcg gcttcacatc ttcaacgata gtcacatcca ccctcatcag
    28681 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
    28741 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
    28801 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
    28861 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
    28921 aaaatgataa tcaaagaaca gccccctatt tctgacacct acagatggca agaaatagcg
    28981 cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
    29041 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
    29101 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
    29161 gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg ttatgtcagt
    29221 cgcgggatct ccgatcgcgc atgccaacag gaaggctata cccacgcgat cccctttggt
    29281 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
    29341 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
    29401 aggggatctt cgctgattaa agaaatagcg
//
LOCUS       DAZFTN010000058        28909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFTN010000058 DAZFTN010000000
VERSION     DAZFTN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            214..1080
                     /gene="folD"
                     /locus_tag="ACHKV2_002782"
     CDS             214..1080
                     /gene="folD"
                     /locus_tag="ACHKV2_002782"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO6940645.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1082..1294
                     /gene="ybcJ"
                     /locus_tag="ACHKV2_002783"
     CDS             1082..1294
                     /gene="ybcJ"
                     /locus_tag="ACHKV2_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO6940646.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1402..1923
                     /gene="ybcI"
                     /locus_tag="ACHKV2_002784"
     CDS             1402..1923
                     /gene="ybcI"
                     /locus_tag="ACHKV2_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6940647.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1959..3344)
                     /gene="cysS"
                     /locus_tag="ACHKV2_002785"
     CDS             complement(1959..3344)
                     /gene="cysS"
                     /locus_tag="ACHKV2_002785"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO6940648.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3518..4012
                     /gene="ppiB"
                     /locus_tag="ACHKV2_002786"
     CDS             3518..4012
                     /gene="ppiB"
                     /locus_tag="ACHKV2_002786"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO6940649.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4015..4737
                     /gene="lpxH"
                     /locus_tag="ACHKV2_002787"
     CDS             4015..4737
                     /gene="lpxH"
                     /locus_tag="ACHKV2_002787"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO6940650.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4855..5364
                     /gene="purE"
                     /locus_tag="ACHKV2_002788"
     CDS             4855..5364
                     /gene="purE"
                     /locus_tag="ACHKV2_002788"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO6940651.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5361..6428
                     /gene="purK"
                     /locus_tag="ACHKV2_002789"
     CDS             5361..6428
                     /gene="purK"
                     /locus_tag="ACHKV2_002789"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO6940652.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6567..7460)
                     /gene="ybcF"
                     /locus_tag="ACHKV2_002790"
     CDS             complement(6567..7460)
                     /gene="ybcF"
                     /locus_tag="ACHKV2_002790"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6940653.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7457..8272)
                     /gene="ylbF"
                     /locus_tag="ACHKV2_002791"
     CDS             complement(7457..8272)
                     /gene="ylbF"
                     /locus_tag="ACHKV2_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6940654.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8283..9542)
                     /gene="ylbE"
                     /locus_tag="ACHKV2_002792"
     CDS             complement(8283..9542)
                     /gene="ylbE"
                     /locus_tag="ACHKV2_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6940655.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9552..11219)
                     /gene="fdrA"
                     /locus_tag="ACHKV2_002793"
     CDS             complement(9552..11219)
                     /gene="fdrA"
                     /locus_tag="ACHKV2_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6940656.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11536..12585
                     /gene="allD"
                     /locus_tag="ACHKV2_002794"
     CDS             11536..12585
                     /gene="allD"
                     /locus_tag="ACHKV2_002794"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO6940657.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12607..13842
                     /gene="allC"
                     /locus_tag="ACHKV2_002795"
     CDS             12607..13842
                     /gene="allC"
                     /locus_tag="ACHKV2_002795"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO6940658.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VAPLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13853..14638
                     /gene="allE"
                     /locus_tag="ACHKV2_002796"
     CDS             13853..14638
                     /gene="allE"
                     /locus_tag="ACHKV2_002796"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO6940659.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14866..16011)
                     /gene="glxK"
                     /locus_tag="ACHKV2_002797"
     CDS             complement(14866..16011)
                     /gene="glxK"
                     /locus_tag="ACHKV2_002797"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO6940660.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16033..17334)
                     /gene="ybbY"
                     /locus_tag="ACHKV2_002798"
     CDS             complement(16033..17334)
                     /gene="ybbY"
                     /locus_tag="ACHKV2_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO6940661.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17391..18752)
                     /gene="allB"
                     /locus_tag="ACHKV2_002799"
                     /pseudo
     CDS             complement(17391..18752)
                     /gene="allB"
                     /locus_tag="ACHKV2_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(18789..20180)
                     /gene="ybbW"
                     /locus_tag="ACHKV2_002800"
     CDS             complement(18789..20180)
                     /gene="ybbW"
                     /locus_tag="ACHKV2_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO6940662.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(20349..21227)
                     /gene="glxR"
                     /locus_tag="ACHKV2_002801"
     CDS             complement(20349..21227)
                     /gene="glxR"
                     /locus_tag="ACHKV2_002801"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6940663.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21328..22104)
                     /gene="hyi"
                     /locus_tag="ACHKV2_002802"
     CDS             complement(21328..22104)
                     /gene="hyi"
                     /locus_tag="ACHKV2_002802"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO6940664.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22117..23898)
                     /gene="gcl"
                     /locus_tag="ACHKV2_002803"
     CDS             complement(22117..23898)
                     /gene="gcl"
                     /locus_tag="ACHKV2_002803"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO6940665.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(23988..24803)
                     /gene="allR"
                     /locus_tag="ACHKV2_002804"
     CDS             complement(23988..24803)
                     /gene="allR"
                     /locus_tag="ACHKV2_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO6940666.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24881..25363)
                     /gene="allA"
                     /locus_tag="ACHKV2_002805"
     CDS             complement(24881..25363)
                     /gene="allA"
                     /locus_tag="ACHKV2_002805"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO6940667.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25593..26519
                     /gene="allS"
                     /locus_tag="ACHKV2_002806"
     CDS             25593..26519
                     /gene="allS"
                     /locus_tag="ACHKV2_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO6940668.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26588..27682
                     /gene="mnmH"
                     /locus_tag="ACHKV2_002807"
     CDS             26588..27682
                     /gene="mnmH"
                     /locus_tag="ACHKV2_002807"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO6940669.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27798..28163
                     /gene="ylbG"
                     /locus_tag="ACHKV2_002808"
                     /pseudo
     CDS             27798..28163
                     /gene="ylbG"
                     /locus_tag="ACHKV2_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28725..>28909)
                     /locus_tag="ACHKV2_002809"
     CDS             complement(28725..>28909)
                     /locus_tag="ACHKV2_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940670.1"
                     /translation="DDGGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGD
                     GVNLFLLVKLMLRIIL"
BASE COUNT         7127 a         7843 c         7180 g         6759 t
ORIGIN      
        1 taaacaagga aataaatcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacctcag cgacagtgcg cctaaccttc gcaagaggtc gcttcacgcg
      121 ataaatctga aacgaaacct gacagcgcgc cccgcttctg acaaaatagg cgcatcccct
      181 tcgacctacg taacagatgg aatcctctct ctgatggcag caaagattat tgacggtaaa
      241 acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag ttcaggcgcg tattgcagcc
      301 ggactgcggg caccaggact ggccgttgtg ctggtgggta gtaaccctgc atcgcaaatt
      361 tatgtcgcaa gcaaacgtaa ggcttgtgaa gaagtcgggt tcgtctcccg ctcttatgac
      421 ctcccggaaa ccaccagcga agcggagctg ctggagctta tcgatgcgct gaatgccgac
      481 aacaccatcg atggcattct ggttcaactg ccgttaccgg cgggtattga taacgtcaaa
      541 gtgctggaac gtatacaccc ggacaaagac gtagacggtt tccatcctta taacgtcggt
      601 cgtctgtgcc agcgcgcgcc gcgtctgcgt ccctgcaccc cgcgcggtat tgtcacgctg
      661 cttgagcgtt acaacattga taccttcggc ctcaacgccg tggtgattgg cgcatcgaat
      721 atcgttggcc gcccgatgag catggaactg ttgctcgcag gttgcaccac caccgtgact
      781 caccgtttca ctaaaaatct gcgtcaccac gtagaaaatg ccgatctgtt gatcgttgcc
      841 gtcggcaagc cgggctttat tcctggtgac tggatcaaag aaggcgcaat tgtgattgat
      901 gtcggcatca accgtctgga aaatggcaaa gttgtgggcg acgtcgtgtt tgaagacgcg
      961 gctaaacgcg cctcatacat tacgcctgtt ccgggcggcg ttggcccgat gacggttgcc
     1021 acgctgattg aaaacacgct acaggcgtgc gttgaatatc atgatccaca gggtgagtaa
     1081 catggcgaca ttttctttag gtaaacatcc gcacgttgag ctgtgcgact tgctgaaact
     1141 ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg attgccgaag gccaggtgaa
     1201 agtcgacggc gcggttgaaa cgcgcaaacg ctgcaaaatc gtcgccgggc agacagtgag
     1261 ttttgcaggt cacagcgtac aggttgttgc ctgatccctt tcccggcctg tatcaacagg
     1321 ccggttctct tttcagactt ttgccctcta cgccccgttc atccatgctg atagcatcga
     1381 cattttgccg aagcgaccat catgccaacc gttattacgc acgccgccgt tcccctatgc
     1441 attggtttag ggttgggttc gaaagtcatc cctccgcgcc tgttatttgc cggaatcatc
     1501 ctggcgatgc tgcccgatgc cgacgtcttg tcgtttaatt ttggcgttgc ttacggcaat
     1561 gtttttggtc atcgcgggtt tacccattcg ctggtgtttg cgtttgttgt cccgctgtta
     1621 tgtgtgctga ttggacgtcg atggttcagg gctgggctga ttcgctgctg gctgttttta
     1681 accgtctcgt tgctatcgca cagcttgctg gattcggtaa ccactggcgg taaaggggtt
     1741 ggctggctgt ggccgtggtc agatgaacgc tttttcgctc cctggcaggt gattaaagtc
     1801 gcgccgtttg cattgtctcg ttacaccaca gcgtacgggc atcaggtgat tatttctgaa
     1861 ttgatgtggg tgtggttgcc ggggatgcta ctgatgggaa tgttgtggtg gcgcagacga
     1921 taaccggatg cgaaaactcg catccggcaa tagcgcaatt acttacgacg ccaggtggtc
     1981 ccttgcgggc catcttccag cacgatcccc atctcattaa gacggtcacg cgccgcatct
     2041 gccgccgccc agtctttcgc tttacgggca tccagacgct gttgaattaa cgcttcaatc
     2101 tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca ggaacgcttc cggttcttgc
     2161 tccagcaggc ccaatacggc ggaaagttta cgcaggtgag atgccattgc attcgccgct
     2221 gccatatctt ctactttcag acggtttact tcacgcgcca tatcaaacag cacggaatag
     2281 gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa agcgcgcttc aaacgcttcg
     2341 ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg cagtgtagag acgctccagc
     2401 gccgcacgcg cctgcttcag gttctcttcg ctatagttca gctggctgcg atagtggccc
     2461 gacatcagga agtaacgcac ggtttccgcg tcgtagtatt tcagcacatc gcgcacggta
     2521 aagaagttac ccagcgattt ggacatcttc tcgcggtcaa ccatcaccat ccccgagtgc
     2581 atccagtagt tcacatactg accatcatgg gcacaggtgg actgcgcgat ttcgttttcg
     2641 tggtgcggga acatcaggtc tgaaccgccg ccgtggatat caaagtggtt acccagctgc
     2701 ttgcagttca ttgccgaaca ttcaatgtgc cagccaggac gacccgcgcc ccacggagac
     2761 ggccagctcg gttcgccctc tttcgacatc ttccacagaa cgaagtccat tgggttgcgt
     2821 ttgtcgtcga ccacgtcaac gcgcgcgcct gcctgcagct ggtcgagatc ctgacgcgac
     2881 agcacgccat aagttggatc ggtcgggacg tcgaacatca cgtcgccgtt gtccgccaca
     2941 taagcgtgac ctttggcgat cagttgttca gtgagttcaa taatttctgc gatatggtgc
     3001 gtcgcgcgcg gctccatatc cgggcgcaga atgttcaacg catcaaaatc tttgtgcatt
     3061 tcggcgatca tgcgatccac cagcgccaca aagctttcgc cattttcatt ggcgcgtttg
     3121 atgattttgt cgtcgatatc ggtaatgttg cgcacatact tcagcttata gccgaggaaa
     3181 cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac gcccgtgacc gatatgacag
     3241 agatcgtaaa cggtgattcc acacacgtac atgccgactt ccccggcgtg aataggctta
     3301 aattcctctt tttggcgtgt cagagtattg aagattttta gcatcgaaga ttccgtttag
     3361 acatgtgtgg gtaattgagt tgcgtataat acccatattt cccgcgcgaa tcagcataca
     3421 ttgcgtgatg atccgatcgt gcggttatgc tataacacca ccctatatat gacccgaact
     3481 gggttgaagc accaatcaaa cggaacagga tgcaaaaatg gttactttcc acaccaatca
     3541 cggcgatatt gtcatcaaaa cttttgacga taaagcacct gaaacagtta aaaacttcct
     3601 ggactactgc cgcgaaggtt tttacaacaa caccattttc caccgtgtta tcaacggctt
     3661 tatgattcag ggcggcggtt ttgaaccggg catgaaacaa aaagccacca aagaaccgat
     3721 caaaaacgaa gccaacaacg gcctgaaaaa tacccgtggt acgctggcaa tggcccgtac
     3781 tcaggctccg cactctgcaa ctgcacagtt cttcatcaac gtggttgata acgacttcct
     3841 gaacttctct ggcgaaagcc tgcaaggttg gggctactgc gtgtttgctg aagtggttga
     3901 aggcatggac gtggtagaca aaatcaaagg tgttgcaacc ggtcgtagcg gtatgcacca
     3961 ggacgtgcca aaagaagacg ttatcattga aagcgtgacc gttagcgagt aatcgtggcg
     4021 acactcttta ttgcagatct tcatctctgc gtggaagaac cggcgatcac cgccggtttt
     4081 ctgcgttttt tagcgggaga agcccgcaag gccgacgcgc tgtatattct tggcgatctg
     4141 tttgaagcat ggattggcga cgacgatccc aacccactcc atcgccagat ggcggcagcg
     4201 atcaaagcgg tgtccgattc cggcgttccc tgttatttca ttcatggcaa ccgtgatttt
     4261 ctgctcggca aacgctttgc ccgtgaaagt ggcatgacgt tattgccgga agaaaaagtg
     4321 ctcgaacttt atggtcgtcg ggtgctgatt atgcatggcg acacgctgtg caccgatgac
     4381 gcgggttatc aggcttttcg caccaaagtc cacaaaccct ggctgcaaac gctattcctc
     4441 gctctgccgt tgtttgtgcg caaacgcatt gccgtgcgaa tgcgcgcgaa cagtaaagaa
     4501 gccaacagca gtaaatcgct ggcgatcatg gacgttaacc aaaacgcggt agtcagtgcg
     4561 atggaaaaac atcaggtgca atggctgatc cacgggcata cccatcgccc ggcggtgcat
     4621 gaacttatcg ccaatcagca acctgctttt cgcgtggtac tgggtgcctg gcatacggaa
     4681 ggttcgatgg tgaaagtcac ggcggatgac gttgagctga ttcattttcc gttttaaaaa
     4741 acccgcaact ttgctgattt cacagccacg caaccgtttt ccttgctctc tttccgtgct
     4801 attctctgtg ccctctaaag ccgagagttg tgcaccacag gagttttaag acgcatgtct
     4861 tcccgcaata atccggcgcg tgtcgccatc gtgatggggt ccaaaagcga ctgggctacc
     4921 atgcagttcg ccgccgaaat cttcgaaatc ctgaatgtcc cgcaccacgt tgaagtggtt
     4981 tctgctcacc gcacccccga taaactattc agcttcgccg aaagtgccga agagaacggt
     5041 tatcaggtga ttattgcggg cgcaggcggc gcagcgcacc tgccaggcat gattgccgcc
     5101 aaaacgctgg tgccagtgct gggcgtgcca gtacagagcg ccgcactgag cggtgtcgat
     5161 agcctctact ccatcgtaca aatgccacgc ggcattccgg tgggtacgct ggcgattggt
     5221 aaagctggcg cggcaaacgc ggcattactg gcagcacaaa ttcttgcgac tcatgataaa
     5281 gaactgcacc agcgtctgaa tgactggcgc aaagcacaaa ctgatgaagt gctggaaaac
     5341 ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct cggtaacggg cagttaggcc
     5401 gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt ctggccggtc gggctggacg
     5461 ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac cgctgagatc gaacgctggc
     5521 cggaaacagc attaacccgc gagctggcgc gccatccggc ctttgtgaac cgcgatgtgt
     5581 tcccgattat tgctgaccgt ctgactcaga agcagctttt cgataagctc cacctgccga
     5641 ctgcaccgtg gcagttactt gccgaacgca gcgagtggcc tgcggtgttt gagcgtttag
     5701 gtgagctggc gattgttaag cgtcgcactg gtggctatga cggacgcggt caatggcgtt
     5761 tacgcgccga tgaaaccgag cagttaccgg ctgaatgcta cggcgaatgt attgtcgagc
     5821 agggcataaa cttctctggt gaagtgtcgc tggttggcgc gcgctgcttt gatggcagca
     5881 ccgtgtttta tccgctgacg cataacctgc atcaggacgg tattttgcgc accagcgtcg
     5941 cttttccgca ggccaatgcg cagcagcaag cgcaagccga agagatgctg tcggcgatta
     6001 tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg ttttgtcacc ccgcaaggtc
     6061 tgctgatcaa cgaactggca ccgcgtgtgc ataacagcgg tcactggaca caaaacggtg
     6121 ccagcatcag ccagtttgag ctacatctgc gggcgattac cgatctgccg ttaccgcaac
     6181 cagtggtgaa taatccgtcg gtgatgatca atctgattgg tagcgatgtg aattatgact
     6241 ggctgaaact gccgctggtg catctgcact ggtacgacaa agaagtccgt ccgggccgta
     6301 aagtggggca tctgaatttg accgacagcg acacatcgcg tctgtccgcg acgctggaag
     6361 ccttgatccc gctgctgccg ccggaatatg ccagcggcgt gatgtgggcg cagagtaagt
     6421 tctgttaacc tggtgctcat ttgattgccg gatgcggcgt gaacgcctta tccggcctac
     6481 accgatccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt
     6541 gcgccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc
     6601 agcgtctctt caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca
     6661 taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct
     6721 aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcattcccca gttttcatat
     6781 accgcatcag catcggtgag gatcaccagt ccatcggcgt taatttgctc ggcgagcaac
     6841 gcagcggcga gatctttatc aatcactgct tcactccctg ctccgtcatc cgtcacaggc
     6901 acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct
     6961 tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca
     7021 tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa
     7081 accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga aacttcaatg
     7141 cgcgtccgca ccgtcgtgac aggcggcatc tgcggctgtg cgctcaaact ctgcgccagc
     7201 atatagccaa tcatcccctg actttctgcc accagcacat ccagcggata cggttctacc
     7261 tctttccacg ccagattctg caatgccagc agccccacct gcggcccgtt gccgtgaaca
     7321 atcgccagcc gataagaacg ggccaggcgc gccagcgcgg gtacagcact ggcgatattg
     7381 cgatattgat tttctgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga
     7441 gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca
     7501 gcagtgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg
     7561 tacgtttcgg gcaactcaga gcatgaacaa agtgcaggag tggcgaggcg aaatatcctt
     7621 gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttgc agattgtcgg
     7681 aacaggcaaa aaacggacga ccggagcgcg catctaaagc accataatac caggccgcca
     7741 gcagcattcc gctcagcgtg tcatcatgac ttggcgttaa tccggggcct ttacccagcc
     7801 agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga cgaaactggc
     7861 gtaactcagc aggcagcgga tcgcttgccg ccaacgccag tggcccgaaa agcccggttt
     7921 cctccgcgcg ttgcatccat gcagctgcaa gtggttgagg atgcgcaggc ggcgtaatac
     7981 gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga atcccttgag
     8041 ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg aattgcgcac
     8101 ggcgaagcac ccatcctccg gggccgaaac cactaccctg acgatgcagc gttaacagtt
     8161 cgccgctttc cgtcatcagg ttaatcgccc gccgccacac tcccgctaac cgccaggact
     8221 ggcgataatt cggtgcgcta ctactggcaa gcagaggatg gatgatcgtc attacgcgtt
     8281 cctcaaccaa tccccatgct ttcagccagt gcttccagcg cctgttcaaa gcacgccagc
     8341 ggtgcccgca cggtgcctgc gccaatctgc ccgatccccg cctctttatg ggcgataccg
     8401 gtattgatga gtggcgtaat gccggtttct accacgcgac gaatgtccag tcccaggcac
     8461 gcgccctgaa aatcccagct tgggatctgc aactgcatat tgcgttcaag gtaaatttcc
     8521 gccatctctt cagataccgc cctggccgct tccatgccac tcgcaccgac aaagcgcgtt
     8581 acgccaggcg ctgcgatcat tgccgcacct ccgataccaa aggtttcggt aatcgcgcta
     8641 tcgcccatat ccgggttcgc ctgctcctgc gagaagccgg tgaaaaacag accttgcgga
     8701 gtgtttacag gcgcagtaaa ccagcgttcg cccagcccgc ttacccgaat cccgaacata
     8761 ttgccgttgc gggtcattgc cgtgacgatg ctgcctgcgc ggatcatcgc gccagcatcc
     8821 atcgccgcct tgcagtaagc catcgcgagg ttgaggaaga actgatcggt cacgctgagg
     8881 aaatccatca cttccgcgat gtgctgttta tcatgatcga ggcgagcaat ttgtggggcc
     8941 agcgcacgca ttaacagtgc tgaggaagca atattgcgtt gatggaactc atcgcccatc
     9001 gtaatgccct gcgccatcat cgcagtaaga tcgataccgc gctccatgcg ccccagcgcc
     9061 gcgcttaata ctggcattaa cacatcgcgc atccagcgat ggcgagtcag gacatcttcg
     9121 ccgtaagcgc caaaacgcat cactttgccg ataccttcgt tgaggttgca gtacgcccgg
     9181 ttgccgtcgg tcacgttctc gaccaccagc atcggcatac tggcagaagt aataccgccc
     9241 attggcccga cggcattcac atggtgacaa ggaatgaagt tcacttcccc ctgctccagt
     9301 attgccagcg cctgcgcttc atctttcgcc caaccttcga acagacatgc gcccacgcac
     9361 gcccctttca tgggtccggt catctcctgc cagcgcattg gcggcccggc gtgaagcagt
     9421 gttttgccct cgtttagttc gctgataagt gaagaagccg gttgcacatc cagccagtgt
     9481 ggacgagcgc gacgaatttg ttcgataacc gcagcattgg cttgcgccac tgatgtaaac
     9541 atgagaaccc cttattgcaa acgttctaat aaacgagcca gttttttatt gccgccggcg
     9601 actggcgacc attggtaatg cacaaccggt ttgctggcgc tttgtagctc cagcgcaaaa
     9661 ctgcgtaatc cgatgttaat cacggcgacg ttttccagta atgacggtgt gtgttgctgt
     9721 gcggcaggcg agagcggatg aattaacgct gccgccagca aggtggcttc cggtagcgaa
     9781 ctcacgaccg caatccccgc atcttccagc gtggcgattt gctgcgagcg gcattgcggg
     9841 tcacgttcag tgcctgtcac cgtggcaatg gcatacagtg gttgattgtc tgaacgcgca
     9901 gcacaggctt tttgccaggc gcttaccagc gaggcggcag gatcggcggt cgcaccgaag
     9961 ccaatcacga catcaagcag cagcacacgc acttgcggtt tagcgccgag atcggtaatt
    10021 aactggttgc gtaaggttgg gtcgatcatc ggatggggac gcccgacggt gtagaaatca
    10081 tcgccgaggt caataatctg gtggccatcg gcgtccagca tcatgccgtg atgatgggca
    10141 tcatcggctt ccacgccaag gtgtccggca agtaatcccg ccgcttcggc agccagcgta
    10201 ccgccggtat acaaaccgca aataaatccg ctgctggcag gcattatcgc gttacgtcgc
    10261 gccgtaacgc gtgaaagcag gcaagccagg cgtgcggcct catccagcga ggaggcaaac
    10321 cagacattct cgtcgcgggc caccgccggg gtataaccta aaaacagcgc caccgtcggt
    10381 ttgccggttg ctttcatggc attaacaatt ttcagacgca ccgcttcggc aggtggtttt
    10441 gaaacaaatg ccagcacttc gcttttctcg tctgcactga gcatttccag cgccgtcagc
    10501 gcactgatgc cgcccacttc acggctgagg tcgcggccgc caaggccaat cgcgtgagta
    10561 attccctccc ctgccagcgc aatctgcgag cacagctcct gaatcccggt accggaagcg
    10621 ccaatgacgc caatgttgcc ttccggcatc acgttagcaa aagccagcgg cgtgccggca
    10681 atcatcgacg taccgcagtc cggccccatc accagcaagc ctttttcccg cgcgcgggtt
    10741 ttaagttgga tttcatcttc cagcgtgacg ttatcggaga acatcatcac gttgaggttg
    10801 cgatccagcg cctgattcgc cagctccgcc gcatactcgc cagccactga aatcagcgcc
    10861 agattggcat cgggtaattt ctgacaggca ctgtcccagc gacgcacctg cgtcaacgcc
    10921 tggctgctgc ctgacccctg cgccagttgt tttagcgcct cttcaagctg ctgcataatc
    10981 gcctgcgcga tccccgcatc cgccgcttcg ctacgaattg ccacgcaaat atcgttcggc
    11041 gtggcgttat taaaatcgtc atgccagaaa cctgtggtat ctaataacgc tttattggcg
    11101 ggcgtaccca tcattacgga aacatcatca acattttctg attcgctgag ttttcgtgaa
    11161 ataatcatta aactgaccga atcctgaaaa cacccttttt taataaaggc gtggatcata
    11221 acaactcctt aaaaattgca cccatggcaa ttgcacgagc agtgttaatg tggcgattac
    11281 ggtatcgatg acaacgagta aataaggtga tgaatatcac acagggatta ttaacaaaag
    11341 aattcaaata accgccatgc tattacgtgt tattttttaa taagcgtggt tgttttaaaa
    11401 accaggttaa aagcgagcgg attatcaaat aaattctaac tatatttttt tgcctgtctg
    11461 gatcacataa tccagatatt ttccctgtat gttaattgca gtcatgcttc acaccgtcgt
    11521 taaaaaggaa gacagatgaa aatcagtcgg gaaacactcc accagctaat tgagaataaa
    11581 ctctgccagg ctgggttaaa acgtgagcac gctgcaaccg tggctgaagt attggtttat
    11641 gccgatgcca gagggatcca ctctcatggc gcggtgcgcg tggaatacta cgcggaacgc
    11701 atttcaaaag gcggcaccaa ccgcgaaccg gagtttcgtc ttgaggaaac cgggccgtgc
    11761 tcggcaattt tacatgccga caatgccgcc ggacaggtcg cggcgaaaat gggtatggaa
    11821 catgccatca aaaccgccca gcaaaatggc gttgcggtgg tcggtatcag ccggatgggt
    11881 cacagcggcg caatctctta ttttgtgcag caggcagccc gcgccggatt aattggcatt
    11941 tcgatgtgcc agtccgatcc gatggtggtg ccgtttggcg gcgcggaaat ttactacggt
    12001 actaacccac tggcctttgc cgcgccggga gaaggcgacg agatccttac ctttgatatg
    12061 gcgactaccg tacaggcatg gggaaaagtc ctcgacgccc gctcgcgtaa tatgtctatc
    12121 ccggatacct gggcggtcga taaaaacggt gcaccaacaa ccgatccgtt cgcggtacat
    12181 gctctgctcc ccgcagccgg gccgaaaggg tatggcctga tgatgatgat tgacgtcctc
    12241 tcaggcgtct tactcggctt accgttcggg cgacaggtta gttcgatgta tgacgattta
    12301 cacgccgggc gtaatttggg gcaattacat gtagttatta acccgaactt tttctcctcc
    12361 agcgaattat tccgtcaaca tcttagccag accatgcgcg aattaaatgc cattaccccc
    12421 gcgcccggtt ttaatcaggt ttattatccc ggacaggatc aggatattaa acaacgccaa
    12481 gccgccgtcg aaggcatcga aattgttgat gatatttacc agtatttaat ttccgacgcg
    12541 ctttataaca cgtcatacga aacgaaaaat ccctttgcgc aataattatt gagacaggac
    12601 tttcttatga ttacacattt tcgtcaaacc atagaagaag cgctgccctg gctttcctct
    12661 tttggcgctg acccagcggg tgggatgacc cgtttacttt attcgccgga atggctggaa
    12721 acccagcagc aattaaaaaa aagaatggcc gcaagtgggc tggaaacacg tttcgatgaa
    12781 gtggggaatt tatacggtcg cctgagtggc accgaatatc cacaggaagt gattctgagc
    12841 ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt cggcgcgctg
    12901 gcggcgcggc tggcaattga ctggctgaaa actcaatacg gcgcaccgct gcgtacggtt
    12961 gaagtggtgg cgatggcgga agaagaaggt agccgcttcc cgtatgtttt ctggggcagt
    13021 aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga tgccaaagga
    13081 aatagttttg tcgatgcgat gaaggcttgc ggatttactc tgccggacgc cccgctaact
    13141 ccgcgtcagg atattaaagc ctttgtcgaa ctgcatatcg aacagggctg tgtgctggaa
    13201 agtaatgggc aatcaattgg cgtggtgaat gcaattgtcg ggcaacgtcg ctatacggtg
    13261 acgctgaacg gcgaatcaaa ccatgcaggc accacgccga tgggttatcg tcgtgatacg
    13321 gtttacgctt tcagtcgcat ttgccatcag tcgatcgaaa aagcgaaaaa gatgggcgat
    13381 ccgctggttc tgacctttgg gaaagtagag ccgcgcccga atacggtgaa tgtggtgccg
    13441 ggtaaaacca cgttcaccat tgattgtcgt cataccgacg ccgccgtgct gcgtgatttc
    13501 acccaacagt tagaaaacga catgcgggcg atttgcgatg aaatggacat tcgtattgat
    13561 atcgatttat ggatggacga agaacccgtg ccgatgaata aggacctggt cgcccccctg
    13621 acagaattgt gtgaaagtga aaaactgaat taccgggtga tgcacagtgg tgccgggcac
    13681 gacgcgcaaa ttttcgcgcc tcgcgtacca acctgcatga tttttatccc cagcatcaac
    13741 gggatcagcc ataacccggc ggaacgcacc aatattaccg accttgccga aggggtcaaa
    13801 acgttggcac tcatgcttta tcaacttgcc tggcagaaat aaggagtcat aaatgggata
    13861 tttaaataac gtcaccggtt accgcgatga tttactggct aaccgtgcga ttgtgaaaca
    13921 cggtaatttc gcactgttaa caccagacgg tctggtaaaa aatattattc cgggctttga
    13981 aaattgtgac gcgacaatcc tctccacgcc aaagctgggt gcctcttttg ttgattatct
    14041 ggtcacactg catcaaaacg gcggcaacca acagggcttc ggtggcgaag gcattgaaac
    14101 gttcctgtat gtgatctctg gaaatatcac tgccaaagcc gaaggcaaaa cattttcctt
    14161 aagcgaaggt ggctatcttt attgcccgcc tggctcctta atgacgtttg ttaacgccca
    14221 ggccgaagac agccaaatct ttttatataa gcgccgctat gttccggtag aaggctatgc
    14281 accgtggctg gtttctggca atgccagcga actggaacgc attcactatg aaggtatgga
    14341 tgatgtgatc ctgctggatt tcctgcccaa agagttaggt tttgatatga acatgcatat
    14401 cctttctttt gcaccaggtg ccagccacgg ttatatcgaa acacatgttc aggaacacgg
    14461 tgcctatatt ctttccggtc agggggttta taacctcgac aataactgga tcccggtgaa
    14521 aaaaggcgat tacatcttta tgggcgctta ttctttacag gctggttatg gcgtagggcg
    14581 tggtgaagcg ttcagctata tttactcgaa agattgtaac cgcgacgtag agatttaagt
    14641 cataccactc gcctgattat ttttagctat gttggatttt gccacggttt ataccgtggc
    14701 tttttttgca acatagcctg atgcgcttcg cttatcaggc ctacatgatc tctgcaatat
    14761 atagaatttg cgcgattttc aggaaggata tggtactcat gccgcatccg gcatgagtac
    14821 tgcactctta tgcgatataa atcgcatccg ctttaaaggt aagggtcagt ttttaattcc
    14881 ctgacctatt ttaatggcgc aggcaatatt tcgcgcgctg ttgaagagat tggtttcacc
    14941 gctggcgagc acttcggcta aaggtgccag acgtggcaaa atgctgaata ccgcatcaat
    15001 gccgtactgg tgcaccactt ccacgccatc acccaatacg ccagcaatcc caatcaccgg
    15061 tacattaaac tgcttcgcca ccgacgcaac gcccagcggc gctttaccgc ctgccgtttg
    15121 cgagtcaatg cgcccttccc cggtgatcac cagtgctgcc ccctgcaccg cctgctccag
    15181 gttgaccgcc cgcagtacaa tctcaatacc cggtttaata tccgcattga ggaataccgc
    15241 cgccgcaatc cccataccgc ccgcagcgcc accgcccgcc atctggcaga cattaatttc
    15301 agtttgctgt tgtaaaacac gggcgtaatt ttgcagcccc tgttcaagtt cttcgaccat
    15361 ctccggcgtt gccccttttt gcgggccaaa caccgccgcc gcgccgcgtg cccctaccag
    15421 cgggttatca acgtcacatg ctacttcaat atggcagttc gccagacgcg gatcgcattc
    15481 ctccatctca atgcttgcca cgcgcgctaa attgccgccg ttagccacca gcacctgacc
    15541 gtcggcatca aggaaacgca caccgagcgc ctgcgccatg cccataccgc cgtcgaccgt
    15601 cgcactgccg ccaatgccga gaataatatg gcgaatgccg ttatccagcg catgacgaat
    15661 taactccccc gtaccaaaac tggaggccag caacggatta cgcttttcag gcgcgaccag
    15721 catcaggcca cttgctgccg ccatctcaat caccgccgtt ttcccgtcgc cggtaatgcc
    15781 ataaaaagca ttcacttttt cgcccatcgg cccgcagact tcaagcgtca cgatgttgcc
    15841 gcccgtcgcg gcgaccatcg catccaccgt cccttcgccg ccatccgcta tcggcaaaca
    15901 gatatagttc gcatcgggaa agagggtcga aaacccggct ttaattgcct gacaacattt
    15961 ttctgcactc aagctctctt taaaagagtc tggcgcaatg acaatcttca ttatcaatcc
    16021 tttttcaaca aattattcga tgcgttccca cggaataagg ttatccataa gaacagccag
    16081 taaaatcccg accaataagc cgttactgag cagaggacga agcgttaatg gaaggtcttg
    16141 cagatacaca ggcggtaatg ccattaaaaa tatgccgaca aataacggca atgcgagtcg
    16201 ataaatattg cgagcggtaa acgttatttg ctggctaaac accagcgcgg aaaagagtaa
    16261 aggtagataa gaaaccagca tgaccgcact actcacgggt aaagggatac tgcaaaacag
    16321 tcgcgtgagt gccggaacca gcgccaccag caggcaaata acgctgccat aaataaacga
    16381 acgccgcgtg taatcgccag tctgggttaa taaaccaatg gatgaaacaa acggtgaaaa
    16441 tggaattact gccagcggca cggttatcag tgtcataaat ccggtcgcca caaagctacg
    16501 acgataacgc gtattccccg cgccctgctg cggataaaaa acatccgtgc cccgaatcgc
    16561 accgtaggta ttgctgatat tcaccagacc tgtaatcacc gccgtcagaa taatccccgg
    16621 cgacaaagca ccgccactgc cgagcgggaa ccactgccag tgcaactcac cggagagcga
    16681 atgcgaagaa ggaaagcaaa agtaccacaa caaccagccg gttatggtgc cgaccagcag
    16741 gccataacgg gcaaaacgtt gcggcagaaa gataatcatc gccagtacca ggcacatcac
    16801 cgccaccgag agcgcgaacg gcggtaactg aattttaaaa ttcgggtcgg ctatgccaaa
    16861 cggcagcccg agcatacctt tgaaaaagat agtggtcagc tgcgcgccca gcatcaacat
    16921 aaacaagacc atcaccgacg gcgtaaataa ccgcgccagg cgatgaccta atccgctaaa
    16981 accaatcaac atcgtcagca cgccggagag tgcaatcccc accgccaggc tggtagcgat
    17041 atcgttgatc ggtgtcccgc gcgatgcttc gccaagggtg atagttagga tggttcccca
    17101 ccacaggccc cccggccctt ccataatagc gcgacgatga ccgcaaaacg cctgagcgaa
    17161 gcaggccagt gcggtagcaa gaaaagcata ttgcgtgagc gtaagcaggc tactttgcgg
    17221 caactgaaaa gcagaaagta gcgtaggagg aaccacaacc gtgttgcaaa aaataaagaa
    17281 aaaccactga aatcctgata acaggctttc gcggctaact gcaaaattga acataaacac
    17341 accttaaccg gagaatgccc gccgcaagga cgggcattgc aggggccaga ttactgctga
    17401 tgtttaagga taaattgacc tttcggcgca acagggaagc cctgttcgat gtcgtaaatc
    17461 acatcaccac gtaagatggt tttcgtgata cgcgcgccaa tggtacggcc aacatacggg
    17521 ctgactttgt ggcgatattc cagatcgtca ttggtaagaa catagctgct attcggctga
    17581 atgaagacga agtcggcatc ttttcctggg gcgatacggc ctttttgctg cagaccaaaa
    17641 atatctgctg cgttagtcgc cattaatttg ccgaacattg gcagagacat tccgcgtttc
    17701 tgtaccgctt catcgaacat cacgtccata cagttttgca gaccggcaat accgccccat
    17761 gcttccatga tgttgccggc tttcatttcc ggagggcatg gtgagtggtc ggaaaccagg
    17821 cggtcgattt caccgttaaa cagtttttcc cacatgcctt tctagttttc cagatcgcgg
    17881 atcggcggtg aacacttcgc cagagtaccg atttcttcga actgatcggt atccagtacg
    17941 aagtaatgcg ggcaggattc acaagtaacg tcctgacctt cctgacgtgc acgagtcact
    18001 tcttcaacgc cttccgggct gctgatatgg caaacgtgca gacggcaacc ggcaactttc
    18061 gccagataca gcacgcggcg aatcgcttcc acttcggtaa ataccggacg cgaagccaca
    18121 tagtcatggg cagttacgcg accttcacgt ttcgcttctt cgcccagtgc gtcacagatc
    18181 agcgcgtttt cgcagtgcac cagcaccggc tgccccagtt cgcccagctt ctgcgcacct
    18241 ttgaagaact gccagtcgtt tacatcacgg aagtcgttgt cgataccgcg atcgccacag
    18301 gtcgcaacga agcatttgaa gccgacaacg cccacttcat ccagctcatg cagacggtcg
    18361 atgttgtaag acaccaggcc accgagttgt gccgcatcga tagtcagctt gcctttagcg
    18421 gcatcgaact tcagttcaat tgaagcgcgg tcaaccgttg caggcagctg gttgagcggc
    18481 atttcgatca tggtggtgat accacctttt gccgctgcgc gagtaccggt ttcataacct
    18541 tcccagtggc tacgacccgg ttcagaaata tgggtgtgcg catcaaccat gcccggcgaa
    18601 accaccagac cagacgcatc cataacttct tttgcatcgc ccagatcctg accgatagca
    18661 gcaattttgc cgcctttaac ggcgatatct acaacgcgag cttcgttttc taaaataacg
    18721 gtgccgtttt taatgattaa atcaaaagac ataacaaact ccttgtaaaa tacagattgg
    18781 tttttaactc aagcaaaggc gacgatgacg ccgacaaacc atgaaacacg cgataacggt
    18841 tccattaagg gaataaactt accgccaaga gataaaataa cggccaccag agttactgaa
    18901 aacgcggtga ggttaaaacc gttatcgtaa tatttataat cgccaggtgc ggtgtacagt
    18961 tcatcaagat taatttgccc acgcatcacc acaaaataat gcgccatcat gacaccaatt
    19021 accggaccaa gcattccgcc gataatatcg aggaacagat aaatactgtc ctgattttcc
    19081 attaatttcc acgggcagat cagcaggctg ataatactgg caatcagtac gccgttttta
    19141 taggtcagtt ttgtcggtgc aatggcagca atctgataac cagccggaat aatattacca
    19201 gtggcgttag tggagatagt cgtcatcaga ataaccagta ccgcaaagaa cgaggcaaac
    19261 aggctgtccc aacgctgaac aatatccagc acgttccagg tatcagcgcc gtaatgaata
    19321 ctggctccgg caataataca tacccccgcg accgcaaaca gaatataggc cacaactaaa
    19381 cccagcgttt gccccagcgc ttgctcacga aacgagtggg cgttttgcgt aaagtcggat
    19441 gcgctcaccg ccggtgccgc ccagaccgca actaccgcgt taatcaccac caggaacaag
    19501 aatccactgt tttctgcttt ctgaataccg ctcggaatgt agtcaaagat tggaccgagc
    19561 ccgaccagcg aaatcgccca aatcgccata ccgccgaaaa cgatatagat gcacgggtta
    19621 agaatggcag tgaatttatt taaaactttg ccaccgccaa aacctatacc aacgttgacc
    19681 agccagaaga gtaagaaagt aattaagccc ggtagagaaa ggcctaacag agtgaaatca
    19741 ccaccgagag tcaaaaatcc cggccagatt ttgccaatca gaatcaagca ggccagtgac
    19801 cccgcgtaac attgcaggcc aaaccacata atggcagcaa taccgcctct taataatccg
    19861 ggaaacagtg cgccacgtac gccgtaagaa gcacgcagga tcatggcaaa cggtacgccg
    19921 tatttactgc ccgcagcgcc gtttaatacc attaccgcgg caatgaaaaa ggcgctgagg
    19981 ataattgcca gcataatact aaaggtagac aagccgagaa taaaaaagcc gccgaccatc
    20041 acataattgg gaacgttatg aaccgaaccc atccataagg taaaatagtt aaatgttttc
    20101 caggtccgct ggctttgcgt tttcggcaat agatcttcgc tatagccgcg ttgctggaat
    20161 agttttctct gatgttccat aaatccctct taatttcacg tatcgaataa cgttgtttta
    20221 atctagctat gaggaaaaaa taaaataaaa atattatttt taatactgaa gcaacatctt
    20281 tggctgcttt cttttttaat attgctggcc gattcatttt ttgatatcgg ccatttttat
    20341 tgcgggtatc aggccagttt atggttagcc attaattcca gcgcctgcac taacgcagag
    20401 tgatccaact ggctgccacc gtttgccgcg caggtattaa ataactcctg gcaggtcgca
    20461 gtgtttggca ggttcagagc cagtgccttc gcactttgca gtgccaggtt gagatctttc
    20521 tggtgcagcg caattttgaa acctggatta aaggtgcgtt taatcatgcg ctcaccgtga
    20581 acttccagaa tacgtgagga agcaaagccg cccatcagcg cctggcgcac acgtaccggg
    20641 tctgcaccgg cttttgaagc aaacagcaga gcttcagaga ccgcttcaat attgagcgct
    20701 acgataatct gatttgccac tttgcaggtt tgaccgtcgc cgttaccgcc cacaagggtg
    20761 atatttttac cgagcagttc aaacagcggt ttaacacgtt caaataccgc ttcgtcaccg
    20821 ccaaccataa ttgacaacgt cccttcacgc gcgccgattt cgccgccgga gactggcgca
    20881 tcgagataat caccacccag ttcattcacc tgacgagcga aacgtttagt ttcaatcggt
    20941 gaaatggagc tcatatcaac aatggttttg cccttcagcg aggctttggt acaaccattt
    21001 tcaccaaaca gaacttcttc aacctgaggt gtgtccggca ccataataaa aatgatgtcc
    21061 gctgcttccg ttacctggcg cgcggtgtcg acattaacag caccgagtga cagtaattca
    21121 tcagcaaccg gtccaatggt cgtgacatgt aattgatgac cggcacgcgc cagattaatg
    21181 gccatcggag tacccataat gcctaagcca ataaatccca gtttcataat taacctcttt
    21241 ttaaatgtcg ctattcctga attcagacaa cacgatcccc tgccaactta gcagcctaaa
    21301 aaagcattgt ctgaatagcg ttacgtttta acggtacgga tccatccagc gtaaaccggc
    21361 ttccgtggtg gtttggggtt tatattcaca cccaacccaa ccgttgtagt cagaattttc
    21421 gattacctta aagagataat cataattaat ttctccggtc cccggttcgc cgcgatgcgg
    21481 attatcggca atttgcaggt gaccaatttt atcagcccat tgcgtcatag tgttggttaa
    21541 ttcaccttcc atccgctgca tgtgataaat gtcatactga attttcaaat tgcagcaacc
    21601 aacatcatca atcaatttca gcgcctgccg agttccggtg agatggaaac caggaatatc
    21661 aaaatggtta ataggttcaa tcagtaataa aatatcttct ttcatcagca tattcgcggc
    21721 ataacgcagg ttttctacaa gcgttgcgtg aatctgttca ctgctgaaac cagccggcgt
    21781 tttaccgacc agacagttaa tatttttatt acccagcgca cgggcataac gaatcgctgc
    21841 tgctacgcca tcccgaaact cttcttcacg gccaggaata caggcaatac cgcgctcccc
    21901 cgccgcccag tcacccgccg gtaaattgtg cagcgtatgt tcgagtttat tactcgccag
    21961 cacctgtttt aattcttcaa tgtcgtagtc ataaggaaac ataaattcaa cgccgcgaaa
    22021 accacactgc gcagctttct caaaacgggc gagaaaatca tattcaccaa ataacatcga
    22081 taaattagca gagaaacgta acattattta tctcccttat tcatagtgca tgaagcaggt
    22141 ttcagtcggt gcatccgctg cgttatcggc gatatcttca aattccatga cgttatccag
    22201 ttcgctgccc atcgaaatat ttgtcacacg ctcgagaata acttccacga ctaccggtac
    22261 ccgatattgc gccattaagg ctttcgcctg ttcaaaggcc ggtgcaatat cttccggttt
    22321 gaagacgcga atagctttac aacctaaacc ttccgctact tttacgtggt cgacgccgta
    22381 accgttcact tcgctggagt tgatattctc gaaagcgagt tgcacgcagt agtccatgtc
    22441 aaaggcgcgt tgtgactgac gaatcaggcc gagataggcg ttattcacca gcacatggat
    22501 gtacggaatg ttgaactgcg cgccaacggc taactcttca atcaggaact ggaagtcgaa
    22561 gtcaccggaa atcgccacca cattgcgttt cggatcagcg gcacaaaccc ccagcgccgc
    22621 cggaatcgtc cagcctaacg gaccagcctg accacagttg atccagtggc ggtctttaaa
    22681 gacatgcagc atttgtgccg cagcgatttg tgacaggcca atggtggtga cataacaaac
    22741 atcgcgacca aaggctttgt tcatctcttc atacacgcgc tgcggtttca ccggcacgtt
    22801 gtcgaaatgg gttttgcgca gcaaagtgcg tttgcgctgc tggcaatcgg cgacccattc
    22861 tttacgacac ggcagacgac ccgctttttg catctcctgc gccacttcaa ccagcagtgt
    22921 cagcgccgct ttagcatcag agacaatgcc gagatccgga cacagcacgc ggccaatttg
    22981 cgtcggctca atatcgatat gaacgatttt gcgcccttcg gtgtatttct ctaccgaacc
    23041 ggtatgacgg ttagcaaaac ggttaccgat accaaacacc atgtcggacg ccagcaacgt
    23101 tgcgttaccg taacgatgcg cggtttgcag accgaccatc ccggccatca gttcatgatc
    23161 gtccgggata cagccccagc ccatcagcgt tgggatcacc ggaacgctgg tcagttcagc
    23221 aaactgttgt aacagtgcag ctgcgtcagc attgattacc ccgcccccgg caacaatcac
    23281 cggacgttcg gcctggatta acatttctac agctttttcg atctgcatac ggctggcagc
    23341 aggtttgtag accggcagcg gttcgtacat gtcaggatca aactcgattt ccgcaacctg
    23401 aacgtcgaac ggtaaatcca ccagtaccgg acccggacga ccagaacgca tcaggtgaaa
    23461 tgcctgttgc agcacgcgag gcaccagcgc cgcttcacga actgtaaccg ccattttgct
    23521 gaccggttta gcaattgctt caatatctac ggcctgaaaa tcttctttat gcagacgggc
    23581 gcgcggtgcc tggccggtaa tgcacagaat aggaatggaa tcagcagaag cggaatagag
    23641 cgcggtgatc atgtccgtgc ccgcaggacc ggaagtcccc agacatacgc cgatattccc
    23701 tgccgttgcg cgggtataac cttccgccat gtgcgaagca ccttccacat gacgcgccag
    23761 aatgtgacga ataccgccgt gcttacgcat cgctgagtag aacggattga ttgcagctcc
    23821 cggaacgccg aaggcggtgg tgataccttc tttctccagc acatacattg ccgcgtcaac
    23881 ggctctcatt tttgccattt tattcctacc tccattaatt ggaaaaattt tccaactttc
    23941 aaattttgtc aaaaaaaccc gctccgcaga gcgggatgcg acagacacta tggatgtgct
    24001 ttcagtccca acgccgtgct gatatcgcgg gcggtgtctc tgaccagctc accctgactg
    24061 acaaaacgat cttctgtcag tcttgatgaa ggcccggaga tggagatagc ggcaacaacg
    24121 ctacctacat catcgtaaat tgctgaagct atgcaattca gacctacaac atgctcttct
    24181 ttatctacgg tatagcccag ttcacgcgct tgttccaggt ccttcagcag ggtgggcata
    24241 tccacaagcg tagttggcgt aaactgctgc aaaccggttt gcagaatgat gctcatcaac
    24301 tcctcttccg ccagcggata aagcagcgct ttgcccgcac cggaagcatg cagtggcaga
    24361 cgactgccca gtggcgcgca catcctgacc atcgatttac actctaactg accaattaat
    24421 accgcttcat tgccgttacg gatcgcgaca ttgaccgttt cgccggaaag taacatcagg
    24481 cggcgcataa acggcccggc gacggagagg acatcgcggt tatggatgta cgccgcaccg
    24541 acgttaaaga cacctaatcc tatatgccac cagcctaact gactgtcctg atagacaaaa
    24601 tccgctgcct gtaaaacctt cagcaagcga aaggtcgtgg agagcggcaa atccagattg
    24661 agagaaatat cgctaaccga cgaacttccc ccactttttt ccagatattg cagaatcgca
    24721 attccccgct ctaatgcctg tgcgcccttc tgtgccacag gctccgcctg tcctggcctg
    24781 ccgcgccgtc taacttccgt catacctacg ttaactccga cctacaagtg agcataaaca
    24841 atgaacaatt agtttatctt atgcaagtcg gttgcaggcg tcacgcaaaa cagagttcct
    24901 gttcaggaat actttcaaca tcacagttgt cactgccgcc gcgatcgatg gtcagaaaat
    24961 cggtgacgcg ctgccaggcg aaaagtgggt gatgccagac gttacgatgg taattcactc
    25021 cctgttcgcc gttggtaata aacgcccgca gggttgacag gtctggtttg tcgtcaccta
    25081 acgctacgac caccacaaac acctcacctt tcatcgggat aaaggcctga gtacccagcg
    25141 gatgacgttc gagttcgtga atggtcagcg gcagattcgc cggttgcgct cggttaatgc
    25201 tgataagcgt acggtcttgc tcaagaatct caaccagcgc caaatcgtgg taacgctcca
    25261 ccaggccatt gttaatatgg aaaaaatccc gttgctgcgt ttcgattacg tcgccataag
    25321 cactaaaggc ttcctgactt aacggtaata cctgaagttt catctttttg tccttgtaac
    25381 ataattccgt tcaaccagcg ccgaccggtc agcttggttt caataacagg caatctaaaa
    25441 ccttaagaga acaacatcaa cacaaaactt ggaatatttt tccaaaacta gatttagtcc
    25501 acaaattgaa cagggttatc atgtaaaaat actgataaat cagaggataa gaaaatcacg
    25561 caacttgcgg atttaaaagg ggatacctga agatgttcga tccagaaacc ttgcggactt
    25621 tcattgcggt tgctgaaaca ggaagttttt caaaagcggc agaacgatta tgtaaaacca
    25681 cggcgacgat cagttatcgc attaaacttc tggaagagaa taccggagtg gcgctgtttt
    25741 ttcgcacgac tcgtagcgta acgttgacag cggctggcga gcatctactt tcccaggcca
    25801 gagactggct gagctggctg gaaagtatgc ccagcgagct gcaacaggtg aatgatggcg
    25861 tggaacgcca ggtgaatatt gtcatcaaca acctgctcta caacccccag gccgtcgccc
    25921 agttgctggc gtggctgaat gaacgttacc cctttaccca gtttcacatc tcccgacaaa
    25981 tctatatggg cgtctgggac tcgctattgt acgaaggttt ttcactggct atcggcgtca
    26041 cgggaaccga agcgctggca aataccttta gtcttgatcc cttaggatcg gtgcaatggc
    26101 gatttgtcat ggcggcggat catccgctgg cgaacgttga agagccgcta acagaagcgc
    26161 agttgcggcg ctttccggcg gtcaatattg aagacagcgc ccgcacctta accaaacgcg
    26221 tcgcctggcg attgccaggg caaaaagaga ttattgttcc tgatatggaa acgaaaatcg
    26281 ccgcccatct ggcgggcgtt ggcattggtt ttttgccaaa atcgctttgc cagtcaatga
    26341 tcgataatca acaactggtc agccgggtaa tcccaacgat gcgccctcct tcgccattga
    26401 gtctggcatg gcgcaaattt ggcagcggca aagcggtaga agatattgtg accttgttta
    26461 cccagcgcag gccggaaatc agcggatttt tagaaatttt cggcaaccca cgcagttaaa
    26521 aatcaagttt ccttgcgcgt tggcgcgtaa aatagcgcgc aagctgattt gaggttcact
    26581 tttaactatg caagagagac acacggaaca ggactatcgt gccctgctga ttgctgatac
    26641 gcccattatt gatgttcgcg cccctatcga gtttgagcaa ggcgcaatgc ccgccgctat
    26701 caatctgccg ttaatgaata acgatgaacg cgccgccgtt ggcacctgct ataaacagca
    26761 aggctcagac gcagcgctgg cgctgggaca taaactggtt gcgggtgaaa ttcgtcagca
    26821 gcgcatggac gcctggcggg cagcctgcct gcaaaatccg caaggtattc tctgctgcgc
    26881 ccgtggcggt cagcgctcac atattgtgca aagctggttg catgcagcgg ggattgatta
    26941 tccgctggtg gaaggcggtt ataaggcact gcgccagacc gcgattcagg cgactattga
    27001 actggcacaa aaaccgatag tgctgattgg cggttgtacc ggcagcggta aaacgctgtt
    27061 agtgcagcaa cagccgaacg gtgttgatct ggaagggttg gcgcgtcatc gcggttcggc
    27121 gtttggtcgc acgttacaac cacaacttag ccaggcgagt tttgaaaacc tgctggctgc
    27181 cgaaatgcta aaaaccgacg cccgtcagaa tttgcgcctg tgggtgctgg aagacgaaag
    27241 ccggatgatc ggttcgaatc acctgccgga atgcctgcgc gagcgaatga ctcaggcggc
    27301 gattgcggtg gtagaagatc cgtttgagat ccgtcttgag cgcctgaacg aagagtattt
    27361 cttgcgtatg catcatgatt ttacccacgc gtacggcgac gaacagggct ggcaggaata
    27421 ttgcgaatac ctgcatcacg gactttcggc tattaagcgt cggctggggc tacagcgata
    27481 taacgaactg gctgcaaggc tggatgcagc actgacaacg caactcacca ccggcagcac
    27541 cgacggtcat ctggcctggc tggtgccgtt acttgaagaa tattacgacc cgatgtatcg
    27601 ctatcagctt gagaaaaaag cggaaaaagt tgtctttcgc ggtgagtggg cggaagtggc
    27661 ggaatgggtt aaggcgcagt aatgagggac gtatttgtat tagtcgcgac ttgatctgac
    27721 acatggcctt gaaaggttga gagttaccgg ttttgatatg agtgtcgaat ccttatccaa
    27781 aacaagaggt aactctcatg cttcatactg ccaatcccgt catcaaacac aaagccggtt
    27841 tgctcaatct ggctgaagaa ctcagcaacg tatcaaaagc ctgtaaaatc atgggcgttt
    27901 cgcgtgatac gttttatcgt taccgcgaac tggtcgctga aggcggtgtg gatgcgcaga
    27961 ttaatcgtag tcgccgcgct cctaacctta agaaccgtac cgatgaggca actgaacagg
    28021 ctgttgttga ttacgccgtc gctttcccga cacatggtca gcaccgggcc agcaacgaac
    28081 tgcgtaaaca gggggttttt atctccgaca gtggtgtccg ttctgtctgg ttactccata
    28141 accttgagaa tctcaaaaga cgatactgaa caaattttat caggtgacgt tccgtaaaaa
    28201 gttgtatgga gttctcgatg cagtcggcgc gactatcagt acccccgctg agccctggac
    28261 tatcgggccg ccgtactggg gcatgtcacc ctggcgtgcc gctggttttc cgctaatggt
    28321 ttatttcctc tttctttccc ggcaagaatt tattttaatc cggaaggatt atttattttc
    28381 agatagggac tcacattaaa cacgtaacat tttaattaat gcatggctga atatgtatta
    28441 cacaatgtca gatatcgtaa aagaccagtg caattctatg taaactctct gactgttcat
    28501 tttatttgtt gtttcagggt cggaactcac cctcaataaa caactgaata cgagtagata
    28561 ttcatcgtct gagctatatg gctttacaca atagccgaca tgtcttttct cagtcaggaa
    28621 ttttactttt cctcactacg aagttgacga cttcgatatg ggatagactc ttaattcaag
    28681 caattactct gcacaagcat caggcaatcc agctccaata tttgttataa aataatgcgt
    28741 aacattaatt tcaccaacaa aaaaaggttt acaccatccc cttgccctgg tatcttacca
    28801 tatttacacc acactttttt tgtatctgga tcattctttg atacgatttt cgccacttgt
    28861 tccctggccc tcactgtcaa tgttttctta aacctccccc catcatcat
//
LOCUS       DAZFTN010000059        28262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFTN010000059 DAZFTN010000000
VERSION     DAZFTN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHKV2_002810"
     CDS             complement(<1..209)
                     /locus_tag="ACHKV2_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6940671.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKV2_002811"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKV2_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO6940672.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKV2_002812"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKV2_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO6940673.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKV2_002813"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKV2_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO6940674.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKV2_002814"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKV2_002814"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO6940675.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKV2_002815"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKV2_002815"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO6940676.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKV2_002816"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKV2_002816"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO6940677.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKV2_002817"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKV2_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6940678.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKV2_002818"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKV2_002818"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6940679.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKV2_002819"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKV2_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO6940680.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKV2_002820"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKV2_002820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6940681.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKV2_002821"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKV2_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO6940682.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHKV2_002822"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHKV2_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO6940683.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKV2_002823"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKV2_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO6940684.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKV2_002824"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKV2_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6940685.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKV2_002825"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKV2_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6940686.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKV2_002826"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKV2_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO6940687.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKV2_002827"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKV2_002827"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO6940688.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKV2_002828"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKV2_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO6940689.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKV2_002829"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKV2_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO6940690.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKV2_002830"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKV2_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO6940691.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKV2_002831"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKV2_002831"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO6940692.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKV2_002832"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKV2_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO6940693.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKV2_002833"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKV2_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO6940694.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKV2_002834"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKV2_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO6940695.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKV2_002835"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKV2_002835"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO6940696.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKV2_002836"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKV2_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO6940697.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKV2_002837"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKV2_002837"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO6940698.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKV2_002838"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKV2_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO6940699.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKV2_002839"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKV2_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO6940700.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKV2_002840"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKV2_002840"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO6940701.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6833 a         7591 c         7135 g         6703 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gt
//
LOCUS       DAZFTN010000060        28060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFTN010000060 DAZFTN010000000
VERSION     DAZFTN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(161..1024)
                     /gene="potB"
                     /locus_tag="ACHKV2_002841"
     CDS             complement(161..1024)
                     /gene="potB"
                     /locus_tag="ACHKV2_002841"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO6940702.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(1008..2144)
                     /gene="potA"
                     /locus_tag="ACHKV2_002842"
     CDS             complement(1008..2144)
                     /gene="potA"
                     /locus_tag="ACHKV2_002842"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO6940703.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2394..3620
                     /gene="pepT"
                     /locus_tag="ACHKV2_002843"
     CDS             2394..3620
                     /gene="pepT"
                     /locus_tag="ACHKV2_002843"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO6940704.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3669..4790)
                     /gene="roxA"
                     /locus_tag="ACHKV2_002844"
                     /gene_synonym="ycfD"
     CDS             complement(3669..4790)
                     /gene="roxA"
                     /locus_tag="ACHKV2_002844"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO6940705.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5039..6268
                     /locus_tag="ACHKV2_002845"
     CDS             5039..6268
                     /locus_tag="ACHKV2_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6940706.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6633..6821
                     /locus_tag="ACHKV2_002846"
     CDS             6633..6821
                     /locus_tag="ACHKV2_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6940707.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6879..7433
                     /locus_tag="ACHKV2_002847"
                     /pseudo
     CDS             6879..7433
                     /locus_tag="ACHKV2_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7626..7823
                     /locus_tag="ACHKV2_002848"
     CDS             7626..7823
                     /locus_tag="ACHKV2_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6940708.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            7816..8028
                     /locus_tag="ACHKV2_002849"
     CDS             7816..8028
                     /locus_tag="ACHKV2_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940709.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            8018..8482
                     /locus_tag="ACHKV2_002850"
     CDS             8018..8482
                     /locus_tag="ACHKV2_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940710.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            8475..8708
                     /locus_tag="ACHKV2_002851"
     CDS             8475..8708
                     /locus_tag="ACHKV2_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940711.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            8714..9013
                     /locus_tag="ACHKV2_002852"
     CDS             8714..9013
                     /locus_tag="ACHKV2_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6940712.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            9010..10410
                     /locus_tag="ACHKV2_002853"
     CDS             9010..10410
                     /locus_tag="ACHKV2_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6940713.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            10611..10862
                     /locus_tag="ACHKV2_002854"
     CDS             10611..10862
                     /locus_tag="ACHKV2_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940714.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            10859..11269
                     /locus_tag="ACHKV2_002855"
     CDS             10859..11269
                     /locus_tag="ACHKV2_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6940715.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            11280..11552
                     /locus_tag="ACHKV2_002856"
     CDS             11280..11552
                     /locus_tag="ACHKV2_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6940716.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            11509..11637
                     /locus_tag="ACHKV2_002857"
     CDS             11509..11637
                     /locus_tag="ACHKV2_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6940717.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            11679..11903
                     /locus_tag="ACHKV2_002858"
     CDS             11679..11903
                     /locus_tag="ACHKV2_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940718.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            12155..12361
                     /locus_tag="ACHKV2_002859"
     CDS             12155..12361
                     /locus_tag="ACHKV2_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940719.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            12361..13416
                     /locus_tag="ACHKV2_002860"
     CDS             12361..13416
                     /locus_tag="ACHKV2_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6940720.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            13429..13764
                     /locus_tag="ACHKV2_002861"
     CDS             13429..13764
                     /locus_tag="ACHKV2_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6940721.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            13777..14190
                     /locus_tag="ACHKV2_002862"
     CDS             13777..14190
                     /locus_tag="ACHKV2_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO6940722.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            14396..14938
                     /locus_tag="ACHKV2_002863"
     CDS             14396..14938
                     /locus_tag="ACHKV2_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6940723.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            15194..15475
                     /locus_tag="ACHKV2_002864"
     CDS             15194..15475
                     /locus_tag="ACHKV2_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940724.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(16076..17536)
                     /gene="phoQ"
                     /locus_tag="ACHKV2_002865"
     CDS             complement(16076..17536)
                     /gene="phoQ"
                     /locus_tag="ACHKV2_002865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO6940725.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(17536..18207)
                     /gene="phoP"
                     /locus_tag="ACHKV2_002866"
     CDS             complement(17536..18207)
                     /gene="phoP"
                     /locus_tag="ACHKV2_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO6940726.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(18376..19746)
                     /gene="purB"
                     /locus_tag="ACHKV2_002867"
     CDS             complement(18376..19746)
                     /gene="purB"
                     /locus_tag="ACHKV2_002867"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO6940727.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(19750..20391)
                     /gene="hflD"
                     /locus_tag="ACHKV2_002868"
     CDS             complement(19750..20391)
                     /gene="hflD"
                     /locus_tag="ACHKV2_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO6940728.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(20427..21533)
                     /gene="mnmA"
                     /locus_tag="ACHKV2_002869"
                     /gene_synonym="trmU"
     CDS             complement(20427..21533)
                     /gene="mnmA"
                     /locus_tag="ACHKV2_002869"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO6940729.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(21587..22048)
                     /gene="nudJ"
                     /locus_tag="ACHKV2_002870"
     CDS             complement(21587..22048)
                     /gene="nudJ"
                     /locus_tag="ACHKV2_002870"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO6940730.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(22058..22711)
                     /gene="rluE"
                     /locus_tag="ACHKV2_002871"
     CDS             complement(22058..22711)
                     /gene="rluE"
                     /locus_tag="ACHKV2_002871"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO6940731.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            22883..24133
                     /gene="icd"
                     /locus_tag="ACHKV2_002872"
     CDS             22883..24133
                     /gene="icd"
                     /locus_tag="ACHKV2_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO6940732.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(24247..25389)
                     /locus_tag="ACHKV2_002873"
     CDS             complement(24247..25389)
                     /locus_tag="ACHKV2_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6940733.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(25379..25615)
                     /locus_tag="ACHKV2_002874"
     CDS             complement(25379..25615)
                     /locus_tag="ACHKV2_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6940734.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            25755..26543
                     /locus_tag="ACHKV2_002875"
                     /pseudo
     CDS             25755..26543
                     /locus_tag="ACHKV2_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            26540..27241
                     /locus_tag="ACHKV2_002876"
     CDS             26540..27241
                     /locus_tag="ACHKV2_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6940735.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            27238..27540
                     /locus_tag="ACHKV2_002877"
     CDS             27238..27540
                     /locus_tag="ACHKV2_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO6940736.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            27608..27940
                     /gene="emrE"
                     /locus_tag="ACHKV2_002878"
     CDS             27608..27940
                     /gene="emrE"
                     /locus_tag="ACHKV2_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO6940737.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT         7404 a         6618 c         7215 g         6823 t
ORIGIN      
        1 taccatttgt tgttttttta tagcgatttg ctaaaaggga gaagaaaaaa cgttctccaa
       61 aattagattg caactgtttg attttatgga tagaggcatg ctgtttacta gacagtaaaa
      121 agcatgccaa atttcctatt aaccttttga atataaagca ctaattatct atttctgttg
      181 ccttcttatt cagcagacga gaagcgcgcc agtaaaccag caacatcagg cccattacga
      241 tagtcagcgt aatgctggtg gctgcaccaa acggccagtc acgaatattg aggaactgta
      301 ccttgatgac gttaccgatc agcaggtttt tcgcaccgcc catcaggtcg gacacataga
      361 acaggcccat cgctggcagc atcaccagca gacatccggc aataatcccc ggcatcgtca
      421 gtggaataat gatgcggata aaagtctgta acttgctggc accgagatcg cgcgccgcct
      481 caagcaacgg cttatccagt ttttcgatac tggagtacag cggcatcacc ataaacggta
      541 gcagaatgta aaccagaccg ataatcaccg cactgggcgt gaacatgata cgaattggtg
      601 tgtcgataac gcccagccag agcaaaaact cgttgagata gcctttggtg ctgaggaaaa
      661 ttttcagccc gtagatgcga atcagtgagt tggtccagaa gggaacaatc agcagaaaca
      721 gcagcagcgg acgcaccttg tgtggcagct tcgccagaaa ccaggcaaac ggatagccca
      781 gcaccaggca ggcgagggtg gcgatcagcg ccatattcag cgagtgcaat agcacttcaa
      841 aatagagcgg atcgagcaga cgtgtgtagt tatccagcgt aaagaccatt ttgacaaaac
      901 tggcgtcgtc gcgggccaaa aagctggtgc caatgatcat caggttgggc agaaagacaa
      961 acaacacaag ccaaccgaca atagtgacaa tcactacatt ctggaactta cttgtgttct
     1021 tcatcagcca gtacgacctc ccagctttct acccaattaa tggccatttt ttggtcgaga
     1081 gagtggtcaa agtcaggatc gtcttcattg aagaattcgc tgaccatcac catcttgcca
     1141 ttttccagtt caacaaccga ctccagcgtc atgcctttgt agttacgctc gcgaacgtaa
     1201 ccaatcagcc cttcagcgtg gttgtcgtcg ttaatctctt caacacgtaa gtcttccggg
     1261 cgcagcagaa catgcagttt ttgccccggt tcaacggcga agttaacgta gatattacat
     1321 tcgcggcctt caacgttggc gcgtacgcgc tgctcgtcga gacgttcgat gacagtggcg
     1381 ttaaacatat tgatttcgcc aatgaacccg gcaacaaaca ggtttttcgg ctcttcgtag
     1441 atttcacgcg gcgtgccgtc ttgctcaatg cgaccatcgc gcatcaccac aatcctgtct
     1501 gacatggtga gtgcttcttc ctgatcgtga gtcacgaaga cgaatgtaat gccaagctta
     1561 cgctgtaacg ctttcagctc gttctgcatt tgcttacgca gtttgtaatc cagcgctgag
     1621 agcgactcat ccagcaacaa cagacgaggc ttgttaacca ccgcgcgagc aatggcgacg
     1681 cgttgctgtt gaccaccaga gagctgatgc ggtttacgtt gagcgaaggt ttccagctgc
     1741 accatccgca gggcttccat cacgcggggc gtaatttcag cagcgggggt tttttgcatg
     1801 cgcaacccaa aggccacatt ttcgaacacg gtcatgtggg ggaaaagtgc gtagctttgg
     1861 aaaacagtgt tcacatagcg gttttccgcc ggaacgtggg tgatgtcctc gttatccaac
     1921 atgatgcgtc cggaatcaac agtttccaga cctgcaatca ggcgaagaac ggttgtttta
     1981 ccgcagccag aagggccaag cagcgtgagg aactcgccat tgttgatagt cagatccagc
     2041 tggggaatga cctctttacc atcaaagcat ttgcgaattc ccgccaattg caccagcggt
     2101 gaaagcgaac tcggttgttt attcaatttt ttactctgtc ccatgtaaac gcaacggatg
     2161 gcttaccgat gcggggtttg tggttaacca ccttggtgac tcttaatgag ggcggtaatt
     2221 ctacggcaaa ccgcttgaat cgccaatctt tgttgtgaat tactggctta gctttatatt
     2281 cattaaggta atgctgataa atattcccgc ttgcaggggt aaaagtgacc tgacgcaata
     2341 tttgtctttt cttgcttctt aataatgttg tcacaaaaag tgagggtgac tacatggata
     2401 aactacttga gcgatttttg aactacgtgt ctctggatac ccaatcaaaa gcaggggtga
     2461 gacaggttcc cagcacggaa ggccaatgga agttattgca tctgctgaaa gagcagctcg
     2521 aagagatggg gcttatcaat gtgaccttaa gtgagaaggg cactttgatg gcgacgttac
     2581 cggctaacgt ccctggcgat atcccggcga ttggctttat ttctcatgtg gatacctcac
     2641 cggattgcag cggcaaaaat gtgaatccgc aaattgttga aaactatcgc ggtggcgata
     2701 ttgcgctggg tatcggcgat gaagttttat caccggttat gttcccggtg ctgcatcagc
     2761 tactgggtca gacgctgatt accaccgatg gtaaaacctt gttaggtgcc gatgacaaag
     2821 caggtattgc agaaatcatg accgcgctgg cggtattgca acagaaaaac attccgcatg
     2881 gtgatattcg cgtcgccttt accccggatg aagaagtggg caaaggggcg aaacattttg
     2941 atgttgatgc cttcgatgcc cgctgggctt acactgttga tggtggtggc gtaggcgaac
     3001 tggagtttga aaacttcaac gccgcatcgg tcaatatcaa aattgtcggt aacaatgttc
     3061 atccgggcac ggcgaaagga gtgatggtaa atgcgctgtc gctggcggca cgtattcatg
     3121 cggaagttcc ggcggatgaa agcccggaaa tgacagaagg ctatgaaggt ttctatcacc
     3181 tggcgagcat gaaaggcacc gttgaacggg ccgatatgca ctacatcatc cgtgatttcg
     3241 accgtaaaca gtttgaagcg cgtaaacgta aaatgatgga gatcgccaaa aaagtgggca
     3301 aagggttaca tcctgattgc tacattgaat tggtgattga agacagttac tacaatatgc
     3361 gcgagaaagt ggttgagcat ccgcatattc tcgatatcgc ccagcaggcg atgcgtgact
     3421 gcgatattga accggaactg aaaccgatcc gcggcggtac cgacggcgcg cagttgtcgt
     3481 ttatgggatt accgtgcccg aacctgttca ctggcggtta caactatcat ggtaagcatg
     3541 agtttgtgac tctggaaggt atggaaaaag cggtgcaggt gatcgtccgt attgccgagt
     3601 taacggcgca acggaagtaa gcgaaaaggg atgcggcatg tgatgccgca tccggcttaa
     3661 atccaaactt acccttcgaa gaaccaatac ccgctattga ccagcgccgc gagcatcgcg
     3721 aggaatgacg gatcttccag cgcatcgcca aaattctccg cagtcagcgc aatgttgctg
     3781 gcgagtgcat ccagtgccgg acggtgcggg gaatcgatct tctcaccatt ggtatacacg
     3841 tcgtcgccaa tgcgcaatac gcgcagacca cccaggcgca ccagcacatc accttgtttc
     3901 agcgcatcgt agatttcatc tggctgataa ggcggctccg gcggcgcgat atccagttca
     3961 tgacgtgact gggatataaa ctcgccaaac cattgcttaa agtgttccgg ctggttgatc
     4021 aattcgagca tcatctcacg cagtttatcc atctcttgcg gcagaacgtc cgcaggatga
     4081 gcgcgaggtg gaacatcagg atcgctgtag tagttgccgc ccagttcgcg ttgcagcaca
     4141 taatcggcaa atccgctaat taattcccgc gtatttggcg cgcgaaaacc cacggaatag
     4201 ttcatcgcat tttccagcgc gtagccttca tgcgggaatc ctggcggaat ataaagaata
     4261 tcgccaggct ccagctcttc atcgatgatg gcttcgaacg gatcgacctg taacagatcc
     4321 ggatgtgggc agtgctgttt catttgcagc ttctcgccca ctcgccagcg acgacgtccg
     4381 gtaccctgaa tgataaacac gtcgtactga tcgagatgcg ggccgacgcc gccgccaggt
     4441 acagaaaaag agatcatcag atcatcaata cgccagtccg gtagttcacg gaacggtcgc
     4501 atcagcgcgg cggtcggctc atgccagtgg ttcactgctt gcactaacaa tgaccagtta
     4561 gtttcaccga gatgatcgta gctttcgaac ggaccgtggc tgacctgcca ttttccatcc
     4621 tggtgactga ccagtcgact gtcgacttcg ctttccatcg ccagacccgc caactcgtct
     4681 ggagagatcg ggtcaataaa attattaaag ccgcgtttta acaccaccgg gcgtttctgc
     4741 cagtgacgtt caagaaaatc gggccagtta agagtgagtt ggtattccat gttaagcatc
     4801 cacaggctgg tatctgcaac cgattataac ggatgcttaa cgtaatgcgt gaagtatgga
     4861 catattttat caattagtgt tttttgccgt ttcataatgt ttcagattgt tcattatatt
     4921 tttgattata aacagatttg tgattttcct tgttccagct tgtttcacgt tgtagccctt
     4981 tgtgcaacga tcggtgtagt taatggtgta gttaattgtg atgaccggaa tcaaaattat
     5041 gagcagagca cttaacaaac tgagcgatac acagctgagg aaaatcaacg gcacacccgc
     5101 ccaaaaaaca gcctttctta atgacggtgg aaacctgagc gtcaggcatt caaccagtgg
     5161 ccttttaacc tggtatttca cttacagggc cggaacggga aggggggcac caccggaacg
     5221 cattaagctg ggaaattatc ctgatctgag cctgaaatca gccagggaaa aagccgccca
     5281 gtgtcgcgca tggctggcag aggggaaaaa tccacgtcat gagcttaatt acaccgtaca
     5341 ggaagcgtta aagccggtaa cggttggcga tgcgctcacc tactggcttg agtcgtacgc
     5401 aaaggaaaac cgcgtggatt atgccgccct gaaaaagcgc cttaataatc acgtaataca
     5461 gcacattggt gctatgccgc tggataaatg cgagctacgg cactggctgg cctgttttga
     5521 ccaggtggca aagcgaacgc ctgttactgc cggattcttg ctacagacgt gcaaacaggc
     5581 gcttaagttc tgccggaggc ggcgctatgc aatcagcaac gttcttgatg atatgagtgt
     5641 ggcggacgtt gggaaaaaac cggatataag cgagcgtgtc ttaagcacca aagaactggg
     5701 cgaattattg caggcactgg acaaaaaaat attctccccc tactacatcg cgttaatccg
     5761 cctcctgatt gtgttcggat gccggacggt agaactgagg ttatcggaga tcagcgagtg
     5821 ggattttacc gaaatgctct ggaccgttcc gaaggagcac agcaaaacga aggtagccat
     5881 attccggcct ataccggaag caatactgcc gttcatcacg cagctggtgg agcagaacag
     5941 gcacacgggc ttattgctgg gggaagtgaa acaggaggcc agcgtatcgc agtatggaag
     6001 attagcgcac aggaggctta atcaccctca ctggtcactg catgacatcc ggcgcacctt
     6061 tacaaccatg ctgaacgatt taggcgttga tccgcatgtc gtggagcagc ttacaggcca
     6121 ccagatgcca ggaatgcagc gagtttataa tcattcccgt tatctggatg cgaaacgcaa
     6181 tgcgctggat atgtggacgg agcggttagg gatactggcg ggaacacatg aaaacgtaac
     6241 cacgctacca gtagccagaa gaaaataatt tttttcgtgt tttttcagta tgcgcatact
     6301 ggatatgcga acagatacaa cgtgcaacaa tgagcaacaa tgtggaacaa ctacgaacaa
     6361 gaggcgaaaa agcgtacgga tttataagta gctgattttt aatgtgttac tggtttttta
     6421 tacactagcg aatcactctt taaagagcga gaaaaaaggc atggaacaat attttatgcc
     6481 tttgtattaa catgaattta aaagatttta ttcttacttt ttcattttct gtaaatcata
     6541 catcctcagt atttgccatt catcactcta gcgggaacaa tacgacacaa taagacacct
     6601 gatgactctt taagaaacga aagggggcaa tagtgttaag cactgatcgg tttatacgtg
     6661 aaaaagaatg cgaaaaacta accggcctta gccgtacgtg tcgctaccgc ctggaaaagg
     6721 ccggacaatt cccatcacgt cgtaaacttg gcggtcgttc cgttggctgg tctttatccg
     6781 aggttctggc ctggaaggat agctgcaagg cagttcatta atcacgctgg cggcacacag
     6841 ccgccacaca tcaatcatct gaacacagag ctataaccat gaagattgaa tatacgccag
     6901 aacgtgggcg gggattcgtt cgccctggtg agactggaaa accacaaaat tggggttttt
     6961 caggcataaa aaaagcggcc ccgaaatgga gccgcctttc tgaacagata acccgctgcg
     7021 ccttatgtgt atatgatccc aaacataagc acggggatga tagccgctat caggctggtg
     7081 ggcaatgcta tcagtctggt tcagttcgtt gccatacctg caatgggcgc gtttccctgt
     7141 actctttaag gaattgctca agggcaaaag cacatggcgc gaatctttct gattcatgct
     7201 ctatctttct gcgccgtctt ttccgtgccg gtgataatgt tttggtcaat tctttatcgg
     7261 tcattgtgtt gtcctgcata gcaatgcgcc gtaatacctc acaccacggc gctgatagtt
     7321 tttatccttt gggttctatg ccgcgttgct gtaattcttt gcggagaacg cgtttaatcc
     7381 aagtggctaa agatgtgtcg ccatcggcct tagcagcttc ttctagctgc gctctgaagt
     7441 cttccggtaa tcgcatttgg tatggaggcg aacgtttttc taatggtgtt gacatggtaa
     7501 ttacccttta ttgctatacc tacatggtaa ttaccattgt acttgccatt acacaaaaat
     7561 caacgccccg cagtgctggg aacacatacg gggcgtctaa ccaacaacgt aaactaggag
     7621 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
     7681 ggcgttttct ggcactgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
     7741 aacgcgaagc gcgtaaccgt tgcccgtccg gttgtgttgc tgtatttgcc gcccgtattc
     7801 gtcaggaggt gcgccatgat taatctgtca cttactgacc ttaaccgcat tcagtttcgt
     7861 gagaaattca cagggcagct actggtcaat gtggataaag gtcgcgtggt gtgtaattac
     7921 cacttaccag atgaagcaat tgtcgccaca agggaatcat tacaggaaat cacaaaacgt
     7981 gccggaatga ttaacacgaa tattcagggg atgctttatg cacaataaaa ccacaccgga
     8041 cgcagccgcc gaagccatca aaacgctgat gcacgcgctg attgatattt ctgttatcgc
     8101 ggacagggca cataagcacg ccaccagcga aacagaatat gccggtgctt tcgttcctca
     8161 ctcactggct gtcatgcaat ttagtgctga tacggcgctg aatgaggcca aagctatcct
     8221 gattgctgat tgtgaaaatg ggggggttat gcgtgatgat cgttttaatt ccctgaaaca
     8281 ggaattttcc ggcgttcctg atgatgcggc tgatgcgctt tcgtcaatgc cagaacttat
     8341 tagagcggct tttttcttac tttccacgag agaatataaa tcaacggggc ttgatgtact
     8401 gaatatcgcc gccgattatg cggaatatgt ggcagaggcg cgttatagaa gaaaatttcc
     8461 tgaggatgta agccatgcgt gatatttacc accaaacaat agaccgcgca tttcttgcac
     8521 tttctcacag tgaaaacatg atggaaatat tgcgcatatg gcttgaaaca cttggcgaca
     8581 atgaacgcga caaacaaaaa tcaagaattg ccacggcatt aataacgctt cttgagcctg
     8641 taataatgga actgcaagaa atagatctat tgcacgacag atataaagaa cagcacaccg
     8701 gagaataaaa ataatgaaac ttaaatattc tggcttaact gccagtggca acactcaccc
     8761 taaatttacg cgcggtgata tttaccgcga ccagtacggc ggcacggtaa tgattaaggg
     8821 cgtggaagaa cggcgcgtaa cctaccgccg tgaaggttac gaatatgatt gcgtgatgcc
     8881 tgtttatcag ttccggcgtg atttttctct ggtacagacc gcgccgcata acgtgcccac
     8941 cagcaacgcc agggcacggg caaacatcca gaagctgaaa accatggtta acggattcag
     9001 gggcaagaaa tgaaaagcgc accgaactta aaaaaacagc cttacgacaa gatgaccgaa
     9061 gtcattattt ttgcgggtag tgatgcctgg gcacatgcga aacagtggca ggaacaggac
     9121 gggcgactgg ctggcgataa tgtgcctccc gttgtgctgg ctgatgatca actggatgaa
     9181 ctggcagaac tgagaatcat cgacgagggg cgctattgtg tccggctgta caaggcaggc
     9241 cacatcaggc catcaaatat taatgccatt gcgcacaagc tggcggcggc gggtgtaact
     9301 gatgcgaatt attaccccga agggatgcac agccatatgc gggagaactg gcgcgaatac
     9361 ctggaacggg tgcgcgggaa agagccggcg gaagaaaaaa accaccagcg aaaaaccacg
     9421 ctaccgatga gcgttggatc taccggatac gacacgcaac tggattacgt ggttaagggg
     9481 attattccgg cggtatcgct atgcagcata tacggggcta gcgggtccta taaatcattc
     9541 cttgccggat cgtgggcgtg ccatgttgcc actggtcgcc agtggggagg ccgcagggtt
     9601 gcacatggtg cggttctcta tgtggttggt gaaggcggta taggtgttcc gcgtcgtgta
     9661 aaagcctggg aggttgtgca cgatgagcag gtgaaaaatc tgtatctggt aaaccgcccc
     9721 atctttccgg ctgccccgct tgatgttgat gaaatggtta tcgctgcccg tcaggtggag
     9781 cgggaaacgg gtaaacctgt acgcatgatt attctggata cgctggcgcg ttgctttggt
     9841 gggaatgatg aaaatgattc ccgtgatatg ggggcgttta tccgtggttg tgacgaactg
     9901 aaacgacgca caggggccac ggtgctggtg gttcaccatt ccggcaagga tgagacgaaa
     9961 ggcgcgcgcg gttccagtgc atttcgtgct tcgctggatg ctgaataccg gatacgcagg
    10021 gaggacgcag gaagcgaagc gctggttatc tcatgcacca aaatgaagga cgcggaggaa
    10081 ctcaaagaag ccgcatatga cttacgcgtg gtggagcttt ttaccgacgc tgacggtgaa
    10141 ttaatcacgt cgctggtggt ggtggatgat ccgcgccctc ctgttgaact ggagcgcatc
    10201 gaggaggcag ggaacaagac ggaaaaccat accgcgctat gggggtgcat ccgttcacgc
    10261 acacagaacg gcgacaagtg cacgatcccg ctgttacgtg atgacatgaa aaagctgggg
    10321 tatgaaatga aaaacttccg gcgctggctg tacaagctgg aaaaagatgg ggttattcgt
    10381 atcgatgggg atgatgtagc gccgctataa aagtgaggag caaaagcgag ggggatagaa
    10441 agagggccaa aattagcccg ctctccctca cttttcgacc tgtatacatc ctcaaaagtg
    10501 aggggtaaaa aaatacttat gaaacacaca catagaaaaa ccgaaaatcc caactgcgac
    10561 gaagtgagac gcttgaaaaa gtgaggcgaa aaagtgagag gttgcgagaa atgacccaaa
    10621 aacgcagaga cagaacagag ccaaaatata aagcgttaga catgactgag cacaccttaa
    10681 aggtggcaat cagaacgata gaccgccaca cgcgggaagg atacgcgaag gaacatcccg
    10741 acctgataag cgcattcatg accacggcgg cggcaaactt tgccacgctg acagaacggg
    10801 agattgccga agcggaacag gtaacaacca tcaacgttaa aaccggagag gtggaattat
    10861 gacggcacag atagccgctt acgggcggct ggtggacgac ccgcaggtaa aacagaccag
    10921 caagggcaca ccgatgacgc tggcgcgtat ggcggtatct ttgccatgca gccaggcaca
    10981 ggacggacag gcgacgttat ggttatcggt catcgcattt ggcaaacagg ccggcttcct
    11041 ggctaaacat caaaaaggcg atgttgccag cgtatccggc acgatgcagg taagccagtg
    11101 gaccggacag aacggagaaa cgcggcaggg ctggcaggtt atcgcagaca gcgtaatcag
    11161 tgcccgcgcg gcacgtccag gcgggaacag acgcaaaacc acaggcacac agggtaatca
    11221 gccaccagcg ggaggcgatg acccttacgg tgatgatatt tcgttctgag ggggtgacga
    11281 tggtacatga ccgcatagcg gaggaactgg aggcgaaagg cttttaccgg agggcggcgg
    11341 cgcgatgggg tgaagtcatg cagctggtgg agacagacaa ggaacggcat cacatcacga
    11401 tgcgacggct ggaatgttca aggaaggcac agagggcacc ggagccgccg gataatttcg
    11461 gagacctgaa aaaggcagtc gatcgcactt atgccgaaat gggtatagat ggtgctggtg
    11521 atgaaatatg gcgcaattac caggacagct aatcaaacag ccggagaaat ccggcttttt
    11581 ttgcaccagt tgaaacggta tggcgcatta ccgggttttc gtcacggtca ggcatagtta
    11641 ctatctgaaa caaacagaca caacagagga aaaaaacaat gccgatgaaa tttgatgaga
    11701 tattaaaaca gcgtgataaa taccatgctg acaacatgga gacgatgagc atcaatgatt
    11761 accgcgcatt cctggagacg ggcgcactga ttgaaaagga tcagcatggt tttgtgagat
    11821 gtgctctatc cggtgaaatg ctggcggtaa atcctgaaca gatagatgca ttgatagaat
    11881 ttctgaaaga gatcagagac tgagccagca cacagcacac atagccggag caatccggct
    11941 tttttgcgcc caaaaaaagc ccgataaggt cagagggttc ttatcgggct tttgcatatg
    12001 aggtttttgg atgcactggc gttcgtgatc gggataatca tttcataatt tgcaacataa
    12061 ctcaatatca ttgcataaaa tgcaattctg attataatca ggactggata aacatccagt
    12121 tatgattttt ttagtcgaag aggaatttct tactatggct gaagagaaaa aaagcggtgt
    12181 ttcggtgtac ataagccccg aaatcgtgga ggtgctcaag cagcgccaca aaaaaaacta
    12241 tgaagctggc gttgcggctg gactggatcc gctgatgacg ccggagccgt cgataggttc
    12301 acttgtacgc tcttatttac ttgcggcgct tgggatgcat aaaaattatg ggggtgaata
    12361 atggcaggca aagcaacggc acttaacact aaccagcttt tcatgtacct gaatcgcggg
    12421 gatattgcgg attttaaatt cagccctctg tttaccacgc tgtttttccc gaacgtggcg
    12481 acattcagca cgcaaaacat catgctggat accctggaca ttgaagaagt caccatgtcg
    12541 gcgttttgtt cgcctatggt gggtagccag gttcagcgcg ataaagggta cgaaaccagc
    12601 acaatcaaac ctggctacat gaagccaaag cacgaaatcg atccaacgaa aaccatcatg
    12661 cgcatggctg gagaagatcc ggcacagctt aacgacccta cctatcgccg tatgcgcctg
    12721 attactggca acatgcgccg ccagataaac gccattaagg cacgcgtgga atggctggcg
    12781 gtgaatgcgg taacgaccgg aaaaaacatc attgagggcg aaggcataga acgctatgaa
    12841 atcgactgga aaataccgga aaactgcatc atagagcagg ccaagggtaa aaaatggtcc
    12901 gagcaggata aagacatgca cgacccaatc tatgacatcg agctttatgc tgatcaggct
    12961 ggttgccccg caaacgtcat gattatgggc gctgaggtat ggcgcacatt acgcagcttt
    13021 aaaaaattcc gtgagctgta cgatctttcc cgtggttcag aatccgccgc cgaactggcc
    13081 tgtaaaaacc tgggcgaagt ggtgagcttt aaaggctatc ttggtgatct ggcccttatc
    13141 gtctattccg gcaaatacac tgacagcgat ggtaccgaaa aatatttcct tgagcctgat
    13201 ttgctggtcc tgggtaacac caacaataaa gggctggtgg cctatggtgc gataatggat
    13261 caggaagcgg taagaacggg cgcaacacaa aacatgtttt acccgaaaaa ctggattgag
    13321 gacggcgatc cggcgattga gtacgtgcag acacacagtg caccgcagcc ggtaccggca
    13381 gatattcgca aatttgttac cgtcaaaatt ggttaacggg gggattctat ggacactcca
    13441 tacattgagt tatttgcagg cagtcagcag gtatccacga cgttggtaca ttttgccgct
    13501 gatgctggcg ttattcagga atttaccccg ctgatgctgg cagacaatgg cgagtttaag
    13561 ccgtgggatg gtcaggaatc tggcaaggct gtttatctga cctcgtaccc tgtggacacg
    13621 tcaaagcaga aatcagcaca gtgttacaag acggggatat tcaatattgc cgccgttaac
    13681 tggcctgaga gcgtcgacac cgatgcgaaa aaatgcgccg cctttgcggg ttccggcgta
    13741 tccgttcagc cgctggcccg ataagcaggg ggaacgatgg caacgaatga aagcatcatg
    13801 acgctaccgc tggcgagtaa atttaaagtc gaagcgcggg caatggctga cagaggttta
    13861 tcaacctatg aggccgtata tcaactaaac aaactggaag agcaggacaa gccgtgcgct
    13921 gatgcgatta tggcgcttca tgagcataaa gactatcagc cgctgttgcg tgcaatggca
    13981 aacgtgcctt gtatcgatgt tggtacggct aaaagcatcc ttagcatgac catagagcag
    14041 gaacgtccga aggttgcgcc ggaacttacc gcagcctttg aaaactttat ggacatgcac
    14101 agcccgcaag ccgtatcagc tggcatggca tacgatggca gaaaccaggg cgatgacggc
    14161 gacatcgatc gcatactgaa aaccatctga gacaaggccg gagaaatccg gcttttttac
    14221 gggtccttcc tggaattatg gcccgttacg gggcggcgac ctcgcggatt ttcgctattt
    14281 atgagccttt tcagggtggt ggtggtggtt ttgttgtttg atctatcttt atgaatgaaa
    14341 agggaaagat gcaagcaata caccaacctg aagcagtaat taagttggtg tattaatgaa
    14401 atctcatctg atgaacaaaa aaaacatggc gaaaagctgc cgtgtaagtg cgacagcgtt
    14461 cgacaagtgg ggagtaactc ccgttgagcg taaaggccgt gaggcgtttt atgatgttgc
    14521 cagcgtgata gaaaataggg ttaacaatgc aattagccag cttataaacg acaaaggcga
    14581 gattgacgat gatgaacttt tacgagtcag gatcagatta ctgacagcac aggcagaggc
    14641 gcaggaactt aaaaacgatc gcgatcgcgg tgacgtgatt gatactgagt tttgcctgta
    14701 cgcgctttca aagctggcga gtcagatttc atcaatcatg gacagcctcc cgcttactat
    14761 gcaaaggagc ttcccacaga ttacccccgc catgctggac aggcttaaaa gggaagtggt
    14821 taaagcctgt aatgccagtg ccagagttgc cgacaacctc ccacagatac tggctgatta
    14881 cttgaaagaa acaaccggaa acgtaccgga aaagttgcta cagaagaaag gcgagtaaca
    14941 gacgcgcaat tattgaacaa aaatgagaaa ggatctgata atggcatttt ctggctaaaa
    15001 tgcctcgtgt cagatccttt ctttttttaa tattttcatt gttaaacagt ctgttacaga
    15061 gaagaagatc ggatctgact tttgccggaa aattctcata aacagataaa aaccgcgagg
    15121 tcgccgcccc gtaacgggcc ataattccag gaaggacccg acgacaccag actatcagag
    15181 cgatgggggc acaatgacag aagccgaact actgggatta atccgccgcg tcgccggaat
    15241 cagccagcag catgacgaac aggccacaca gccggacagc gtgaccgctg aaaattatgt
    15301 gcgtgttgta gcggaagtga tgcgccgtga tggtatagaa cttaatggcg ttgatatgcg
    15361 caacatacgg aaccgcgttc ttgaaatgct ggcctacaat cgccgcgttg aactgtatcg
    15421 ggagaaagaa aaaataacgt accactggaa gaagccggaa cggctgcggc ggtaacttgc
    15481 tgatattccc gataacgcaa aattgcgctg gctacatcct cacatctgag gactgttaat
    15541 cagaaggccg gaatatattc acaaactacg aaattttcgt agtttaatta atggtcagat
    15601 taatcgctaa cccgctgata ttttcggaaa cctcaatttg aggaagtcgg aagcgtcacc
    15661 cttcatattc tgaatggtca taatgaccac gcagaccatc aaggcaacca caacgaacat
    15721 cgttacggtt ggagctttta ccccactggg ggaaaggtat tacgataatc ataacaccta
    15781 ccgaacaggt aaaacccacc agcctgatta acagttaacc ggaaaaaaaa tccggtagcc
    15841 ccgttatggg gatccccata tcgacattaa cgcccctcat gaattggtgc gcttccccct
    15901 ggaaagatta cccgcctgta tatttcttgt gtctatttgt tccacgttgt ttcatacagt
    15961 gcaccgaacg gtgtagttaa tggtgtagtc aatttgctgt ttttagcact ttttgcagtg
    16021 tgggttgttt aattaaatca atgggttgcc ttgctgtggt gataaaagga catatttatt
    16081 catctttcgg cgtagaatgc tggcgaccaa aaatcacctc catccgcgca ccgccaagca
    16141 tgctctctcc ggcgacgatt ttaccctcat attgcttggt gatttcgcgg gctaccgcca
    16201 gccccacacc ttgcccaggg cgtaaagtat caacccgttg accacggtcg aaaatgacct
    16261 ctcgcttgct taatggaata ccggggccat catcctcgac cacaatatag agatgctcgt
    16321 cggtttgcct tgcagaaatt tcgacaaact cgaggcaata tttacaggca ttatccagta
    16381 cattgcccat cacctcgaca aaatcgttct gctcaccgac aaagctgatc tctggcgaaa
    16441 tatcgagaga gatattgacc cctttgcgtt gatacacttt gttcagcgcc gaggtgagat
    16501 tgtccagcag tggggcgacc ggatgcagct cgcggctgag caatgtcccg ccgtgcatac
    16561 tggcacgatg caggtagtag ccaatttgct gtgaaatgcg gctgatttgc tccagcatta
    16621 ccggttcagc atcactgacg ctcatctttt cactacgtag agaacgcagc gtactttgca
    16681 gcaccgccag tggcgttttc agactatggg tcaggtcggt gagcgttgta cgatatttgt
    16741 cgtaacgttc gcgttcactt tttaacaatc ggttcaggtt tcgtaccaga ctggtcagtt
    16801 ctcgcgttgt ggctggattg agcaattcgc ggttatgttc ttccagttcg cggacttctt
    16861 ttgccagggc ttcgatgggg cgtaaactcc accaggcggc gacccacagc agcgggatca
    16921 ctaacagcag attggctgag agcacataga taaaccagct ccagaccata taggaacttt
    16981 ttagctccac cggaatggta tccaccacca caatggttaa ctttggcatc cgcgatgttg
    17041 ccgggtagac gtttaccgcc accgagtggg tcatctccgc gtcgtcatca tcttcccgca
    17101 cttcctgcaa ctgttgctgt atcgaatgat ctccactcag caagaggctg gtatcgttaa
    17161 catccgcttc aatttcatga aaaccattcg atttcagcca gtcaggctgg atcatcttca
    17221 tcagccaggg cacgtcacgt tgcgcccata aaagctgccc gttctcatca taaattagcg
    17281 tcatggtggg gctttgcttg tcgatatttt cgggtaactc gacatgcaac ttattgtttt
    17341 cccacttcgc aagggtatag aacagattgc tctcgccacg taacagccga aacgtagttt
    17401 tatcgaaact gacgctataa ccgatcagcg cgaccattcc gtaggcaagc gaaagcacca
    17461 gtactaccgc tgccgttgcc aacagaaaac gtacccgcag cgagagcggg aaaaaaagac
    17521 gcagtaattt tttcatcagc gcaattcgaa caaatagccc tggccgcgaa cggtagtaat
    17581 cacttcttgc ggatattgtg cctgaatttt tttgcgcaga cgtcccatca gtacatcaat
    17641 ggtatggctt tcccgcagct ccgcatccgg atagagttgt agcattaacg aatctttgct
    17701 gaccactttg ccattattgc gtatcaacgt ttccataatg gtgtattcga acgcggtcag
    17761 tttgatcact tcgtcattaa tagataattc acggcgagag agatcaacct gaaacggggg
    17821 gagcgaaatg acctgtgaag ccaggccgct attacgccgc attaatgcct gcattcgcgc
    17881 catcacctct tcaatatgaa acggtttagt cacgtaatca tcagcaccgg cacttaatac
    17941 ttcgactttg tcctgccagc tttcacgggc ggttaatacc agaatcggca gtgaaacatc
    18001 attgctacgc cagcggcaaa tcagtgacag accgtcctcg tctggcaatc cgagatcgac
    18061 aatcgcaata tccggtaaat gttcattgag ataataatcg gcttctttgg catcttctgc
    18121 atcatcgacc tgatgaccag catcctgaat ctgaacttta aggtggtgac gtaacaacgc
    18181 attgtcttca acaaccagta cgcgcatttt tatttctccc tgtcttaatt attaaaatag
    18241 tgtaacgcga ttatgtggtt atgggggtaa acattaaata aaccagcggg ggaggggagg
    18301 taaagtgaaa aaataaaaag cggataatct taataagcag gccggacagc atcgccatcc
    18361 ggcactggta cgagtttatt tcagctcatc aaccatggtg atggcgcgac caatgtagtt
    18421 agccggcgtc atcgctttca ggcgggcttt ctcttcttct ggcaacgcca gaccgtcgat
    18481 aaactgcttc atgccttcgg cgtcaacgcg cttaccgcga gtcagctctt tcagcttctc
    18541 gtacggtttt tcgatgccat agcgacgcat aactgtctgg attggttctg ccagcacttc
    18601 ccagttgtga tccagctcat ccagcagatg gtcacggttc acttccagtt tgctcacgcc
    18661 tttcagggtg gattgatacg caatcagcgc ataaccgata cccacgccga ggttacgcag
    18721 cacggtggag tcggtcaggt cacgctgcca gcgggaaacc ggcagtttgc ttgccagatg
    18781 ctgcaatacc gcgttggaaa ggcccagatt cccttcggag ttttcgaagt cgatcgggtt
    18841 aactttatgc ggcatggtgg aagaaccaat ctcaccagca atggttttct gtttgaagtg
    18901 gttaagggcg atataacccc agacgtcacg gtcaaagtcg atcagaatgg tgttgaagcg
    18961 cgcaacgcaa tcaaacagtt cggcaatgta gtcgtgcggt tcaatctggg tagtgtacgg
    19021 gttccattga atacccagcg aggtgacgaa ctcttcgctg aactgatgcc agtcaacttc
    19081 cgggtaagcg gcgatgtggg cgttatagtt accgaccgcg ccgttgattt tgccgaggat
    19141 ctccacctgg ttaagctggc ggtactggcg ctccatacgg taggcgacgt ttgccatctc
    19201 tttaccgatg gttgacggcg tggctggctg accgtgggta cgggacagca gcgggatatc
    19261 gcgatactga acggcgagat ctttaatgcc atcaattagt tgacgccagt acggcaggat
    19321 cacttcatca cgcgcggttt tcagcattaa tgcgtgggag aggttattga tatcttccga
    19381 agtacaggca aagtggatga attcagaaac cgcgtgcagt tccgggatct ccgccacttt
    19441 ttctttcagg aaatactcaa ccgctttaac gtcgtggtta gtagtacgct cgatggtttt
    19501 gatgcgtgcg gcatcttctt cgctgaaatt ggcgacaatt gcatcaaggt aaccgattgc
    19561 gtcggcagca aaagcaggaa cttccttgat cgctgcgtgc gcggccagtt tttgcagcca
    19621 acgtacttca acttgtacac ggaatttcag caaaccatat tcgctgaaaa tcccgcgcag
    19681 cgcgctgact ttatcgccgt agcgtccatc gacaggggaa acggcggtca gtgaggataa
    19741 ttccatagat cacaactccg gggttaaatg agcaagaatt tgttttgcct gagtggtcag
    19801 gcgattacga gaaaacatca gttgcagacg tccgccgccg acctggtgcc agagcacggc
    19861 ggcgcgaatg cctgccagca gggttgcgcg aactttcgcc tgcacttgtg ggctttgcag
    19921 tacagcaggg gaaccggtga cctgaatgcg cgggccaagc gggctgatca catcaacata
    19981 gatagcagcc atcgcgctca tcagcgtttc ggactgtaaa tcgaagtgtt cgagctggcg
    20041 ttgcaggccg ttgatccggt tgcccagagt gtcgagcgca cctttcgctg aggagagttt
    20101 gcgcttaagc accatcaggc tgagtgtgta gcgggttaat tcggcgttta agccctggcg
    20161 actgctggca ttgagcacgc cgagcagggt ttccagcccg acgcgcaggt tggcttcgct
    20221 accgccaaaa accgccagcg tcgagctggg gttcatatca ataatactgt tgagtgagac
    20281 gtgtagcgca tcggcatcac aatgcccctg gtgagcgagt tgttgcacca ggcgtgccga
    20341 ctgacaaata ccggccaggg cgagggtgat gtcatagtaa ttctttgcca cgttcactgc
    20401 ttccttgttt taagtaaaga taataatcag atcggcagcg gcagacgctg ctcaataata
    20461 ccgccaccga ggcacacttc accgttatag aagacggcag actggcccgg cgtcacggcg
    20521 gcaacgggct catcgaaaat cacttcaatg cgatcatcgt ccagcgcctt gacggtgcag
    20581 gggatatcgg tctggcgata gcgggttttt accgtgcaac gcatagtacc ggtgaatggt
    20641 tcacgatcga cccagtgcag ctgctgggca atcagaccga cagacatcag acgcgggtgt
    20701 tcatggccct gcgcaacaat cagaatgttg ttttcgacgt ctttatcgac cacataccac
    20761 ggttcttcgg tgccttcttt ggtgccaccg atacccagac ctttgcgctg accgagggtg
    20821 tgatacatca gcccctgatg ctcaccaatt tcatcgccat cgactgtaat gattttgccc
    20881 ggttgcgccg ggagataacg gccaaggaac tcgcggaatt tacgttcgcc aatgaagcaa
    20941 atgccggtag agtctttttt cttcgcggtg accagaccaa gatcttcagc aatcttacgc
    21001 acctgcggtt tttccagttc gccgaccggg aacaggcttt gcgcaatctg ctcatggctg
    21061 agcgtataaa ggaagtagct ctggtcttta ttgctgtcca gaccacgcag caggcggctc
    21121 ttgccatcga catctgcccg acgaacgtaa tgaccggtag cgatataatc ggcacctaaa
    21181 tcttcggcgg caaattcgag gaaggcttta aatttgatct ctttgttgca cagaatatcc
    21241 ggattcggcg tgcgaccggc tttatattcg gcaaggaaca gttcgaagac gttgtcccag
    21301 tactcggcag caaagttaac ggtgtgcagt tcaatgccga gcttgtcgca gacagcctgg
    21361 gcatcagcca gatccgctgc cgctgtgcaa tattcctcac cgtcgtcttc ttcccagttc
    21421 ttcataaaca ggccttcgac ctgatatccc tgttgttgca acagccaggc agaaacggag
    21481 gaatcgacac cgccggacat gccgacgatt actttttttg cggtttcaga cattggatca
    21541 ctcacgacat tgaacttcaa ggcggcgtat tctatcacgc agccttttag atgacaccct
    21601 ttgtaaaagg ccagttaaaa tcgccaatca tctccagcgg atagcgttgc ccgctttgat
    21661 aacaacggat actttccgcc accagcggcg aacgaagatt tgacgcctgt aaaatttctt
    21721 cggcgctgac ccaacgacag cagtcgatat cgctgtcatg aggctgggtc gggcatattt
    21781 gctcaagctc aatggcaaag aggaaacgca aaaacggcgt tttatctggc gcaatccact
    21841 gatgcatacg aataaagtgt tgcggctgcg cgctgatgcc ggtttcttcc cacagctcac
    21901 gggcggctgc ttccactaag gtttcatcgg cttccagatg cccggcaggt tggttccata
    21961 aggctttacc attaatcgtc tcttcaacga ctaaaaattt cccctctgcg tgcactacgc
    22021 aagcaacggt aacgtgcggt ttaaacattg tgcatcctta ttctgttgct tctcgccatt
    22081 cgccattggc aaggttatcc aaagagtaat cacccatcgc atagcgaatc agtcgcagcg
    22141 tggggaaacc aacatgggcg gtcatgcggc gcacctggcg attacgtcct tcatataagg
    22201 tgatcttcag ccagctggtg ggaatacttt tgcgttcacg aattggtgga ttccgcggcc
    22261 ataaccacgc gggttcgtca accagctccg cgcctgcggg cagggtaggg ccatcattta
    22321 aggttacgcc attgcgcaag gcttcaagtg cgtcttgtgt gggaatacct tccacctgca
    22381 cataatagat ttttccggtg cgtttgcccg gctgggttaa acgcgcctgc aacgcgccgt
    22441 tattggtcag caccagtaac ccttcgctat cgcggtcaag acgacctgct gcataaacgc
    22501 cctgaaccgg gatgaattct tttaatgttt tgcgtccggc ttcatcggtg aactgcggaa
    22561 gaacatcgta gggtttattg aacaggatca cacgcgtggg ctggttttca ggtttacgcc
    22621 tggtagaacg ttgtgagctg aatcgcttaa cctggtgatt tctaaaagaa gttttttgca
    22681 tggtattttc agagattatg aattgccgca ttatagccta ataacgcgca tctttcatga
    22741 cggcaaacaa tagggtagta ttgacaagcc aattacaaat cattaacaaa aaattgctct
    22801 aaagcatccg tatcgcagga cgcaaacgca tatgcaacgt ggtggcagac gagcaaacca
    22861 gtagcgctcg aaggagaggt gaatggaaag taaagtagtt gttccggcac aaggcaagaa
    22921 gatcaccctg caaaacggca aactcaacgt tcctgaaaat ccgattatcc cttacattga
    22981 aggtgatgga atcggtgtag atgtaacccc agccatgctg aaagtggtcg acgctgcagt
    23041 cgagaaagcc tataaaggcg agcgtaaaat ctcctggatg gaaatttaca ccggtgaaaa
    23101 atccacacag gtttatggtc aggacgtctg gctgcctgct gaaacccttg atctgattcg
    23161 tgaatatcgc gttgccatta aaggcccgct gaccactccg gttggtggcg gtattcgctc
    23221 tctgaacgtt gccctgcgcc aggaactgga tctctacatc tgcctgcgtc cggtacgtta
    23281 ctatcagggc actccaagcc cggttaaaca ccctgaactg accgatatgg ttatcttccg
    23341 tgaaaactcg gaagacattt atgcgggtat cgaatggaaa gctgactctg ccgacgccga
    23401 gaaagtgatt aaattcctgc gtgaagagat gggcgtgaag aaaattcgct tcccggaaca
    23461 ttgcggtatc ggtattaagc cgtgttctga agaaggcacc aaacgtctgg ttcgtgcagc
    23521 gatcgaatac gcaattgcta acgatcgtga ctctgtgact ctggtgcaca aaggcaacat
    23581 catgaagttc accgaaggcg cgtttaaaga ctggggctac gagctggcgc gtgaagagtt
    23641 tggcggtgaa ctgatcgacg gcggcccgtg gctgaaagtt aaaaacccga acaccggcaa
    23701 agagatcgtc attaaagacg tgattgctga tgcattcctg caacagatcc tgctgcgtcc
    23761 ggctgaatat gatgttatcg cctgtatgaa cctgaacggt gactacattt ctgacgccct
    23821 ggcagcgcag gttggcggta tcggtatcgc ccctggcgca aacatcggtg acgaatgcgc
    23881 cctgtttgaa gccacccacg gtactgcgcc gaaatatgcc ggtcaggaca aagttaaccc
    23941 aggttctatt attctctctg ctgagatgat gttacgccat atgggttgga ctgaagcggc
    24001 tgacctgatt gttaaaggta tggaaggcgc aatcaatgcc aagaccgtaa cctatgactt
    24061 cgaacgtctg atggaaggcg ctaaactgct gaaatgttca gagtttggtg acgcgatcat
    24121 caagaatatg taatcactac atgtgttaaa tattgaaacg ggcgtataac acgcccgttg
    24181 ttttatgatg atttaaaatc ttccccaaaa ctttccccaa aacccttccc caaaactggc
    24241 tattttctat gctgttttga tatctacgat aatccagtct ttaccacgat catcattgta
    24301 tcggtcggtc atttttctgg atttatgacc taacaacttt tgcgtatcca gaccctgttc
    24361 ccgatataac cgttctgaca gagatcgctg ctcatgaaat gtgggcgcag ttccttgctc
    24421 ccattttatg ccacattttt ccctggcctt tttaaaagcc gttgtaagag tatttgcaga
    24481 cacctggtct cctctgtttg cttgagaggt agtgtgacgg taatggacca gatatttact
    24541 aacaacagca tccctgcact gagatataac ttcacgaagg gtaatattca gagcatcgca
    24601 tttcaggtta agcggaatag caagttttga accggttttt tcctgagtaa tgtgcaacat
    24661 gtcgtcccag atatcagaga atttcaaatt gcagatatcg cttaaacgtt gtccagtgac
    24721 aagagcaagt agcatcccgc attttaaata gggctgccgt ctgcttacgc tgtcaaatat
    24781 tgcctgccat tcgggcagtg ataatctttg acggtttact cgatttcgcg gttgttttgt
    24841 tgcctgcgct gggttaaatc ctggcggaac atgtcctgcg tgttgtgctt ctttgaagac
    24901 gtcgatcaac accattctca cgacttgcgc catcctgtta tgaccttcag cctttacagc
    24961 atcaattatt tcggcaatat caagtgcggt aatatccttg aggtgttgca ttccacaatg
    25021 ctcacggaaa agacggatgg gtttgccttt ttgccgatag gagttgggtc ttagttcatt
    25081 atgttgcagc ctgtcctcct ggatagaatt atatttatca agccattctg tcaccgtaat
    25141 gtctgagcgc ctgcctttca ttctttccag acgctcattg acgcttaata tttgtcgggt
    25201 acgttgttca gcaataatgg tatttgcttc agtagcaact tgttttgctt cattctcatc
    25261 agttcctaag ctatgaaaac gaccggatag tggatgtttg tattgccaat atacctttcc
    25321 ggttcgctta tctaatttgc aatataaatt gggtatagag attttgtgag atcgggggct
    25381 agcagccatc agcgattatc cgttggagtt ttgggtttgc gtttattggg agttgcggtt
    25441 ctgcaagcgt tcctacaaaa cgggaatttc ggtcaatcat ccagtagcga ccaactttta
    25501 tagcgggtgg ggccatcatt ttcccttgcg cgtatttttt cagaactcgc tcacttggtg
    25561 ctaagtcccc aaattcttct ttagcccagt cccgtaaagt gattagtcga gacatttgtc
    25621 ctcctcttag ctgctgaggg agtttgtgac cgatatatct gacatgatat taagctcatg
    25681 gcaggtacat ctcttgactg gtcatagaga taaatttaat gctgagaaat gcagtattga
    25741 atttatcaat ttttcaccag cttctggtct ttctggctgt catgcgcaaa acatatggct
    25801 ttaataaaaa actggattgg gttagcaacg agcaactttc cgaattaacc gggatattgc
    25861 cgcacaagtg ttctgctgca aaaagtgttc tggtaaagcg tgggattttt attcagagcg
    25921 ggcggaatac cggcattaat aatgtggtca gtgaatggtc aacattaccc gaatcaggta
    25981 agaaaaataa agtttacctg aaagaggtaa atttacctga atcaggtaaa aaaagtttac
    26041 ccaaatcagg taaaggcgtt tacccgaatc aggtaaacac aaaagacaaa ctaacaaaag
    26101 acaatataaa acctttttcg tccgagaatt ctggcgaatc ctctgaccaa ccagaaaacg
    26161 atcttcctgt ggtgaaaccg gatgctgcaa ttcagagcgg cagcaagtgg gggacagcag
    26221 aagacctgac cgccgcagag tggatgtttg acatggtgaa gaccatcgcg ccatcagcca
    26281 gaaaaccgaa ttttgcaggg tgggctaacg atatccgcct gatgcgtgaa cgtgacggac
    26341 gtaaccaccg cgacatgtgc gtgctgttcc gctgggcatg ccaggacaac ttctggtccg
    26401 gtaacgtgct aagtccggcc aaactccgcg acaagtggac ccagctcgaa atcaaccgta
    26461 acaagcaaca ggcaggcatg atagccagca aaccaaaact cgacctgaca aacactgact
    26521 ggatttacgg ggtggattta tgaaaaacat cgccgcacag atggttaact ttgaccgtga
    26581 gcagatgcgt cggatcgcca acaacatgcc ggaacagtac gacgaaaagc cgcaggtaca
    26641 gcaggtagcg cagatcatca acggtgtgtt cagccagtta ctggcaactt tcccggcgag
    26701 tctggctaac cgtgaccaga acgaactgaa cgaaatccgc cgccagtggg ttctggcttt
    26761 ccgggaaaac gggatcacca caatggaaca ggttaacgca ggaatgcgcg tagcccgtcg
    26821 gcagaatcga ccatttctgc catcacccgg gcagtttgtt gcatggtgcc gggaagaagc
    26881 atccgttatc gccggactgc caaacgtcag cgagctggtt gatatggttt acgagtattg
    26941 ccggaagcgt ggcctgtatc cggatgcaga gtcttatccg tggaaatcga acgcgcatta
    27001 ctggttggtt accaacttgt accagaacat gcgggccaat gcgctgactg acgcggaatt
    27061 acggcgcaag gctgccgatg aactgacctg tatgacagcg cgaattaacc gtggtgagac
    27121 gatacctgaa ccagtaaaac aacttcctgt tatgggcggt agacctctaa atcgtgcaca
    27181 ggctctggcg aagatcgcag aaattaaagc taagttcgga ctgaaaggag caagtgtatg
    27241 acgggcaaag aggcaattat tcattacctg gggacgcata agagcttctg tgcacaggac
    27301 gttgccgcgg taacaggtgc aaccgtaacc agcataaatc aggctgcggc taaaatggcg
    27361 cgggcaggaa tcctggtcgt tgatggtaag gtctggcgaa cggtgtatta ccggttcgct
    27421 accagagaag aacgggaagg aaaggtgagc acgaatctga tttttaagga gtgtcgccag
    27481 agtgccgcga tgaaacgggt attgagggta tataaaagaa catcaatggg tacacaatga
    27541 tgaaacaggt gagttgagtt caaactgtag tacaattctc tccagtttga acaggaaaga
    27601 atatgttatg aacccttata tttatcttgg tggtgcaata cttgcagagg tcattggtac
    27661 aaccttaatg aagttttcag aaggttttac acggttatgg ccatctgttg gtacaattat
    27721 ttgttattgt gcatcattct ggttattagc tcagacgctg gcttatattc ctacagggat
    27781 tgcttatgct atctggtcag gagtcggtat tgtcctgatt agcttactat catgggggtt
    27841 tttcggccaa cggctggacc tgccagccgt tataggcatg atgttgattt gtgccggtgt
    27901 gttggttatt aatttattgt cacgaagcac accacattaa aaataatttc ttttaaaaga
    27961 ctgcaatatg gcggttcata tctttacatg ggccgctttt gttaatgttt ttagtttttg
    28021 tgtattcttt tgtgccttca agattattgc gtaagcaaat
//
LOCUS       DAZFTN010000061        27579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFTN010000061 DAZFTN010000000
VERSION     DAZFTN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(102..1469)
                     /gene="rhlE"
                     /locus_tag="ACHKV2_002879"
     CDS             complement(102..1469)
                     /gene="rhlE"
                     /locus_tag="ACHKV2_002879"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO6940738.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1698..2369
                     /gene="cecR"
                     /locus_tag="ACHKV2_002880"
     CDS             1698..2369
                     /gene="cecR"
                     /locus_tag="ACHKV2_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO6940739.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2372..3367
                     /gene="hlyD"
                     /locus_tag="ACHKV2_002881"
     CDS             2372..3367
                     /gene="hlyD"
                     /locus_tag="ACHKV2_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO6940740.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3360..5096
                     /gene="ybhF"
                     /locus_tag="ACHKV2_002882"
     CDS             3360..5096
                     /gene="ybhF"
                     /locus_tag="ACHKV2_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6940741.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5089..6222
                     /gene="ybhS"
                     /locus_tag="ACHKV2_002883"
     CDS             5089..6222
                     /gene="ybhS"
                     /locus_tag="ACHKV2_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6940742.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6233..7339
                     /gene="ybhR"
                     /locus_tag="ACHKV2_002884"
     CDS             6233..7339
                     /gene="ybhR"
                     /locus_tag="ACHKV2_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6940743.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7301..7711)
                     /gene="ybhQ"
                     /locus_tag="ACHKV2_002885"
     CDS             complement(7301..7711)
                     /gene="ybhQ"
                     /locus_tag="ACHKV2_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO6940744.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7844..8605
                     /gene="ybhP"
                     /locus_tag="ACHKV2_002886"
     CDS             7844..8605
                     /gene="ybhP"
                     /locus_tag="ACHKV2_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6940745.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8602..9843
                     /gene="clsB"
                     /locus_tag="ACHKV2_002887"
     CDS             8602..9843
                     /gene="clsB"
                     /locus_tag="ACHKV2_002887"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO6940746.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9843..10799
                     /gene="ybhN"
                     /locus_tag="ACHKV2_002888"
     CDS             9843..10799
                     /gene="ybhN"
                     /locus_tag="ACHKV2_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO6940747.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10835..11548)
                     /gene="ybhM"
                     /locus_tag="ACHKV2_002889"
     CDS             complement(10835..11548)
                     /gene="ybhM"
                     /locus_tag="ACHKV2_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6940748.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11756..12460)
                     /gene="ybhL"
                     /locus_tag="ACHKV2_002890"
     CDS             complement(11756..12460)
                     /gene="ybhL"
                     /locus_tag="ACHKV2_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6940749.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12596..13048)
                     /gene="moaE"
                     /locus_tag="ACHKV2_002891"
     CDS             complement(12596..13048)
                     /gene="moaE"
                     /locus_tag="ACHKV2_002891"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO6940750.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGLVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13050..13295)
                     /gene="moaD"
                     /locus_tag="ACHKV2_002892"
     CDS             complement(13050..13295)
                     /gene="moaD"
                     /locus_tag="ACHKV2_002892"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO6940751.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13288..13773)
                     /gene="moaC"
                     /locus_tag="ACHKV2_002893"
     CDS             complement(13288..13773)
                     /gene="moaC"
                     /locus_tag="ACHKV2_002893"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO6940752.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13776..14288)
                     /gene="moaB"
                     /locus_tag="ACHKV2_002894"
     CDS             complement(13776..14288)
                     /gene="moaB"
                     /locus_tag="ACHKV2_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO6940753.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14310..15299)
                     /gene="moaA"
                     /locus_tag="ACHKV2_002895"
     CDS             complement(14310..15299)
                     /gene="moaA"
                     /locus_tag="ACHKV2_002895"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO6940754.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15287..15431)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15696..16604
                     /gene="yvcK"
                     /locus_tag="ACHKV2_002896"
     CDS             15696..16604
                     /gene="yvcK"
                     /locus_tag="ACHKV2_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO6940755.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16795..18816)
                     /gene="uvrB"
                     /locus_tag="ACHKV2_002897"
     CDS             complement(16795..18816)
                     /gene="uvrB"
                     /locus_tag="ACHKV2_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO6940756.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19395..20072)
                     /gene="bioD"
                     /locus_tag="ACHKV2_002898"
     CDS             complement(19395..20072)
                     /gene="bioD"
                     /locus_tag="ACHKV2_002898"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6940757.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGESPWLAENPENAATGK
                     YINLALL"
     gene            complement(20065..20820)
                     /gene="bioC"
                     /locus_tag="ACHKV2_002899"
     CDS             complement(20065..20820)
                     /gene="bioC"
                     /locus_tag="ACHKV2_002899"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO6940758.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20807..21961)
                     /gene="bioF"
                     /locus_tag="ACHKV2_002900"
     CDS             complement(20807..21961)
                     /gene="bioF"
                     /locus_tag="ACHKV2_002900"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO6940759.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21958..22998)
                     /gene="bioB"
                     /locus_tag="ACHKV2_002901"
     CDS             complement(21958..22998)
                     /gene="bioB"
                     /locus_tag="ACHKV2_002901"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO6940760.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23085..24374
                     /gene="bioA"
                     /locus_tag="ACHKV2_002902"
     CDS             23085..24374
                     /gene="bioA"
                     /locus_tag="ACHKV2_002902"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO6940761.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24433..24909
                     /gene="ybhB"
                     /locus_tag="ACHKV2_002903"
     CDS             24433..24909
                     /gene="ybhB"
                     /locus_tag="ACHKV2_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO6940762.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25227..25319
                     /locus_tag="ACHKV2_002904"
                     /pseudo
     CDS             25227..25319
                     /locus_tag="ACHKV2_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25416..26114)
                     /gene="nleD"
                     /locus_tag="ACHKV2_002905"
     CDS             complement(25416..26114)
                     /gene="nleD"
                     /locus_tag="ACHKV2_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO6940763.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26176..26297)
                     /locus_tag="ACHKV2_002906"
                     /pseudo
     CDS             complement(26176..26297)
                     /locus_tag="ACHKV2_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(26345..27226)
                     /gene="nleH1-1"
                     /locus_tag="ACHKV2_002907"
     CDS             complement(26345..27226)
                     /gene="nleH1-1"
                     /locus_tag="ACHKV2_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFO6940764.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(27396..27551)
                     /locus_tag="ACHKV2_002908"
                     /pseudo
     CDS             complement(27396..27551)
                     /locus_tag="ACHKV2_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         6559 a         7335 c         7022 g         6663 t
ORIGIN      
        1 aaatttatta aaacaataaa atcagacatg tcatgcatgt ctgaccgtgt tacctgaata
       61 atgggcaaaa gcctgtgata ctcttttgcc cttacatggt attactgcgc agcggcaggt
      121 ttacgcggac ggcgacggcg ttgttgttcg cctgccggtt tggcatcgcc aagacgacga
      181 gatggttttt ctgcaggctt cgcgctagct gatttcgcac caccttctcc acggcgtggt
      241 tgctgttgac cgcgaccacc accaccttgc ccacgaccgc cgccgccacg ttgctggcga
      301 ccgttctgga tcggttcggc tttgattgac gggtccggct cataacccgg aatcgcaatg
      361 cgcgggatct cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca
      421 cacaccagcg acaatgcttc gccagttgcc gccgcacggc cagtacgccc gatacggtgg
      481 acataatctt ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata
      541 tccagaccgc gcgcagcgat gtcagttgcc accagtacac gaatatcgcc cgatttaaaa
      601 tcggccagcg cacgggtacg cgcaccttgc gatttattac cgtggatcgc cgcactacgg
      661 atgccatctt tattaagctg ttcagcgaga tggttagcgc catgtttggt acgggtaaac
      721 accagcacct gctgccagtt ccctttccca atcatgtgcg acagcaattc gcgcttgcgt
      781 ttcttatcaa caaagtgaac atgctgggtc acctgatcag acgcggtatt gcggcgtgcc
      841 acttctattt ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag
      901 aaggtcgcgg agaacaacag gttctggcgc ttcgcaggta gttttgttaa cacgcgacgg
      961 atatcgtgga taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca
     1021 acctgatcca gcttcactgc attctgatgt tccaggtcca gcaaacgtcc cggcgttgcc
     1081 accagcacat caacgccgcc gcgcaacttc atcatttgtg ggttaatgct gacaccgcca
     1141 aacaccacca gcgaacgaat gttcaggtat ttgctgtaat cacggacgtt ttcgccaatc
     1201 tgcgccgcca gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg
     1261 gcgtgcggct ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgttttg
     1321 ccggtgccgg tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc
     1381 tgctgctgaa taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca
     1441 gggcttaaac ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg
     1501 tgtagttttc agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag
     1561 cggagtgtag cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa
     1621 aattcttagc catactggac aactcccata tggagtcata atcttaatca atcatttgat
     1681 taagaatgga ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa
     1741 aacagctgat tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc
     1801 gcgagatagc cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga
     1861 aagaagattt gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc
     1921 gtccgcatgc cgaggaagcc gaacgcttgt tcgcccaacc acagcctgat cgggctgcca
     1981 tccgtgaact gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca
     2041 ccgtcaacct cagcaagttt atctcccgtg agcagctctc tcccacggca gcctaccacc
     2101 tggtgcacga acaggtgatt agtccgctac acagccacct gacacgcctg attgccgcct
     2161 ggaccggctg cgacgccaat gacacccgca tgatccttca tacccatgcg ttgattggtg
     2221 agattctggc gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt
     2281 tcgatgaaga aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt
     2341 tgcaaggatt atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc
     2401 ggtagtggta cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga
     2461 taacggcctg acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt
     2521 tggggggcgc gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt
     2581 gctgggcgaa ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggcgt
     2641 ctcggtggca caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc
     2701 tcaggccgcc gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta
     2761 taaccgccag caaggactgt ggaaaagccg caccatttcg gcaaatgacc tggaaaatgc
     2821 ccgctcctcg cgcgaccagg cacaggcaac gctgaaatca gcacaggata aattgcgtca
     2881 gtaccgttcc ggtaaccgtg aacaggacat cgctcaggcg aaagccagcc tcgaacaggc
     2941 gcaggcgcaa ctggcgcagg cggagctgaa tttacaggac tcaacgttga tagcaccgtc
     3001 tgatggcacg ctgttaacgc gcgcggtgga gccaggcacg gttctcaatg aaggtggcac
     3061 ggtgtttacc gtttcactaa cgcgtccggt gtgggtgcgt gcttatgttg atgaacggaa
     3121 tcttgaccag gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa
     3181 gccctatcac gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt
     3241 cgaaacaccg gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc
     3301 cgatgatgcg ttacgccagg gaatgccagt gacggtacaa ttcggtgacg aggcaggaca
     3361 tgaatgatgc cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc
     3421 ccgccgtcgc gccgctcgat tgcaccattc atgccggtta tgtgacgggg ctggtagggc
     3481 cagacggtgc gggtaaaacc acgctgatgc ggatgctggc gggattactg aaacccgaca
     3541 gcggcaatgc cacggtgatt ggctttgatc cgatcaaaaa cgacggcgcg ctgcacgccg
     3601 tgctcggcta tatgccgcag aaattcggcc tgtatgaaga tctgacggtg atggagaacc
     3661 tcaatctgta cgcggatttg cgcagcgtca ccggcgaggc acggaagcag acttttgctc
     3721 gcctgctgga gtttacgtct cttggcccgt ttaccggacg cctggcgggc aagctctccg
     3781 gtgggatgaa acaaaaactc ggcctggcct gtaccctggt gggcgaaccg aaagtgttgc
     3841 tgctcgatga acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg
     3901 tgcatgagct ggcgggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag
     3961 ccgagcagtg ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggagaac
     4021 caaaagccct gacacaaacc atggccggac gcagctttct gatgaccagt ccacacgagg
     4081 gcaaccgcaa actgttgcaa cgcgccttga aactgccgca ggtcagcgac ggcatgattc
     4141 aggggaaatc ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg
     4201 ccgacgggat gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcgttta
     4261 ttgatttgct gggcggtgcc ggaacctcag aatcgccgct gggtgcaata ttgcatacgg
     4321 tagaaggcac acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg
     4381 attttgccgc caccgatcac gtcaactttg ccgttaaacg cggggagatt tttggtttgc
     4441 tggggccaaa cggcgcgggc aaatcgacca cctttaagat gatgtgcggt ttgctggtgc
     4501 cgacttccgg ccaggcgctg gtgctgggga tggatctgaa agagagttcc ggtaaagcgc
     4561 gccagcatct cggctatatg gcgcaaaaat tttcgctcta cggcaacctg acggtcgaac
     4621 agaatttacg ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa
     4681 tctcccgtat gagcgaggca tttggcctga aaagtattgc ctcacacgcc accgatgaac
     4741 tgccattagg ttttaaacag cggctggcgc tggcctgttc gctgatgcat gaaccggata
     4801 ttctgtttct cgacgaaccg acttccggcg ttgaccccct cacccgccgt gaattctggc
     4861 tgcacatcaa cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg
     4921 atgaagcgga atattgcgac cgcatcggcc tggtgtaccg cgggaaatta atcgccagcg
     4981 gcacgccgga cgatttgaaa gcacagtcgg ctaacgatga gcaacccgat cccaccatgg
     5041 agcaagcctt tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg
     5101 atcctttcct ggcgtcgcgt gcgggcgctg tgcgtcaaag agacgcggca gatcgttcgc
     5161 gatccgagta gctggctgat tgcggtagtg atcccgctgt tgctgctgtt tatttttggt
     5221 tatggcatta acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc
     5281 gaagcagcgc tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc
     5341 agcgataacc gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg
     5401 gttattcccg tggatttcgc cgaacagatg gagcgcgcca acgctaccgc accgattcag
     5461 gtgatcaccg acggcagtga gccgaatacc gctaactttg tgcagggata tgtcgaaggg
     5521 atctggcaga tctggcaaat gcagcgggcg gaggataacg ggcagacctt tgagccgctt
     5581 attgatgtac aaacccgcta ctggtttaac ccggcggcga ttagccagca ctttattatc
     5641 cccggtgcgg tgaccatcat catgacggtc atcggcgcga ttctcacctc gctggtggtg
     5701 gcgcgagaat gggaacgcgg cactatggag gctctgctct ctacggagat tacccgcacg
     5761 gaactgctgc tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg
     5821 tgtatgctgg tgtcagtgtt tattctcggc gtgccgtatc gcggttcgct gctgattctg
     5881 ttttttatct ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg
     5941 attacccgca accagttcaa tgccgctcag gtcgccctga acgccgcgtt cctgccgtcg
     6001 attatgcttt ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg
     6061 acgtacatta ttcccgcacg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg
     6121 aatattccag tggtgctggt ggtgaacgta ctgtttttga tcgcttcggc ggtgatgttt
     6181 atcggcctga cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca
     6241 tcgcttatgg acgttaatcc gcaaagagtt gcagtcgttg ctgcgcgaac cacaaacccg
     6301 cgcgattctg attttaccgg tgctgattca ggtgattctg ttcccgttcg ccgccacgct
     6361 ggaagtgacc aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga
     6421 gctgacccaa cgttttgccc gcgccagcgc ctttacacat gtgctgctgc tgaaaagccc
     6481 acaggagatc cgcccgacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc
     6541 tgacttctcg cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg
     6601 gcgtaactcc aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta
     6661 tcagcaggag ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa
     6721 ctggtataac ccgaatctcg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat
     6781 caccactatt ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg
     6841 tacgctcgat cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc
     6901 cgtaccggcg ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg
     6961 ggcgtatcaa atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta
     7021 tggtttatcg ctggtgggat tcggtctgtt gatttcatca ctctgttcaa cacaacagca
     7081 ggcgtttatc ggcgtgtttg tctttatgat gcccgccatt ctcctttccg gttacgtttc
     7141 gccggtggaa aacatgccag tatggctgca aaacctgacg tggattaatc ctattcgcca
     7201 ctttacggac attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa
     7261 tagtttgtgg ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt
     7321 tagacgtaag gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg
     7381 caaggattgc cagccctgca attgccagca aaaagttgtt ttccagtacg cgggacaaca
     7441 gccacagcac aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt
     7501 ctgccacctc acgccagcgt tgctcggggt ggcgctgaaa caccagcatc atcactaccg
     7561 tgacacaata cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc
     7621 ccaccaccag cagcgccact accagtaaat gcaacataat ctgccagcaa cttagacccg
     7681 ttgcgacacg aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac
     7741 ttactcagag taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg
     7801 actacactat ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca
     7861 acaattttcg ttcaaggtgc tcaccatcaa tattcacaaa ggctttaccg cgtttaaccg
     7921 acgcttcatt ttgccggaac ttcgcgatgc cgtgcgtacc gtcagcgccg atattgtttg
     7981 cctgcaggaa gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga
     8041 tacctcgcac tacgagtttc tcgccgacac tatgtggagc gattttgcct acggtcgcaa
     8101 tgccgtatac ccggaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca
     8161 ttatgagaat cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg
     8221 cattgtgccg ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg
     8281 tgaggcgcac cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga
     8341 cggcgaaccg gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc
     8401 gttaaaagtg caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg
     8461 cacgtttccg gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag
     8521 cgccagcgcg ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc
     8581 tttaagtgcg gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg
     8641 ctggaaaacg gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa
     8701 cgcatcattc ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca actgcatgcg
     8761 gcactactgg cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt
     8821 tcgccggatc tcagcgatga gtttgtcaat gaactgacgg cagctggcgt agtgttccgc
     8881 tactacgatc cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat
     8941 cgcaaaattg tggtaatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc
     9001 gagcatatgt ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg
     9061 ccgattgtcg aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca
     9121 cgacgctggt ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag
     9181 gtattgctgg tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg
     9241 aaaatgctca ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc
     9301 tatcgatttt tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc
     9361 attcagggcg aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat
     9421 ctggttaaag gcggcgttca ggtgtttgag taccgccgcc gcccgctcca cggcaaagtg
     9481 gcattgatgg acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg
     9541 tcactgaatc tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc
     9601 gataatctga acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg
     9661 aaacgcacct ggtggaacct aaccaaaagc gtgctggcgt tccacttttt acgccacttc
     9721 ccggcgctgg ttggctggct tccggcacac acgccacgtc tggcgcaggt tgatcctccc
     9781 gcacaaccga caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc
     9841 tgatgagtaa atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt
     9901 ttatcgcggt gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct
     9961 ggaaggtcat ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg
    10021 tcagctatct gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac
    10081 tggcgaagcg ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca
    10141 gtacctgggt cggcggcatt ggtatgcgct atcgtttgta ctcccgtctg ggattgccgg
    10201 gcagcactat tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt
    10261 tactggcagg gattatcttt accgcaggcg tggtggagtt gccggaccac tggtatgtcg
    10321 atcaaactac gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt
    10381 ggttttgcgc tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc
    10441 cttcgtggaa attcgccctc gcccagatgc tgatttccag cgttaactgg atggtcatgg
    10501 gggcgattat ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt
    10561 tgctggttag cagtattgcc ggcgtcatcg tgcatgttcc ggcggggatt ggtgtgctgg
    10621 aagcggtgtt tatcgcgcta ctggctgggg agcatacatc caaaggcaca attatcgccg
    10681 ccctactcgc ttaccgtgtg ctgtattact ttatcccgct gctgctggcg ctgatctgtt
    10741 acctgttgct ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa
    10801 gtttagttaa gttctttagt atgtgcattt acgactaatg aaaaaaacgc gtatgccttt
    10861 gccagacaag cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat
    10921 agagtgttaa tgcacctgta aaagcgcaac gaaccggact ccacaactct tcactatggc
    10981 atttccccgc caattcaagg agcttatttc gtccatgcaa gataatgcct gaccataaca
    11041 caatcattaa gcaacttatt acccaaatgg gccgttcact cattaatgcg gcattcacga
    11101 tgattaccag ggccaaccct gtcaacgtca tcataatcat aaaacggtga gaaccaggat
    11161 caatattaaa taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga
    11221 acacgacgat aatggcatat acaggaacta aacttgcaac gacaatcgcc accaaagaga
    11281 gtaaaaacag atatattacg cctgcgatgg cggcatttat cttgttgata tccttaatta
    11341 agccatacat acaaagtaat aataacccta tggcgacata cattggtggt gaatcatatt
    11401 tggttgccgt aatttcgaga tacttagccc cccaggccag ccctgcggtt gccaccaaac
    11461 ctaaagcggt aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt
    11521 tattgctgtt ttgcaagtat gattccattc ttttagttgt ccttatagaa aaatattaat
    11581 attcatcatg aataaattta aacccacgca tacaaaatac ttttgtattt atggatacgg
    11641 aaaactcttc cgctcggcca taaggcgtat tttatacctg gtacttgtta agtgtcgttt
    11701 ttagtaagaa aaaaaatccc gacacatggc cgggatattg atggtgaaaa aagaattaac
    11761 gacgattgcc aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg
    11821 ttaatgcgca aagaatggaa tatttgcgca ggttcgacgc gtcgcgggta tctatctgct
    11881 cacccatatt tttcagtttc tgggtgtcat acgccgtcaa tccgacaaag acaatcacgc
    11941 cgatgtaggt gactgcccac atcaacgctt cgcttttcag ccagaagttg accagcgatg
    12001 ccaacacaat gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct
    12061 tcgtggtgta accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaggtac
    12121 tggcgataga agcagcggta taaacaatga aaatactgga aagcgtaaga cccgtcagcg
    12181 ccgaataaag cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg
    12241 ataacacaat aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag
    12301 tgaacaacag ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca
    12361 gcaataagcc aacggtcatc cagccataga cttgcgccat ataagtctgc aagccagccc
    12421 ggggttgtac aattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata
    12481 aggaatatct taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca
    12541 tgtactgagc ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaactacc
    12601 agcgttttgc cgcctgctga tcgctctccc gagcttcgac ccagcggtcg ccctccggcg
    12661 tggcttcgcg cttccagaac ggtgcgcggg ttttgagata atccataata aactgcccgg
    12721 cttcaaacgc actgctgcga tgcgcactgg tgacaccgat aaaaacgatt tcatcgcccg
    12781 gccataattc cccgatgcgg tgaatcaccg tgacgagccc cagcggccag cggttacgcg
    12841 cttcatcgac aatttctgcc agtgcttttt cagtcatccc cggatagtgt tcgagggtta
    12901 atgctttgac gctgtcgccc aggttatggt tgcgcacctt accggtaaag gtgactaccg
    12961 caccgtcttc gtcacgctcc gccagccacg ggtactcttc tcctacgctg aacggctgcg
    13021 gaccaacaac aattttggtt tctgccatct taacctccgg ttaccggcgg gaagaaagct
    13081 acttcgtcgc cgtcagtcag cggatggtca aaactcacca gcgtctggtt gacggcagcc
    13141 agtaatttgc catcttccag cgccagcgcc cagcgatcgc tctgcgcagc caggtgctgg
    13201 cgtaacgctt caacggttgg gaaatccgca gccacttcgg ttgcatctgt tcctaccaac
    13261 tcgcgcacct gggcgaaaaa aagaacttta atcatccgct tccaccttaa agtcacccga
    13321 cttgccgccg ctcttcgcca gcaaacgtac cggaccaatc accatatctt tttgcaccgc
    13381 tttgcacatg tcataaatgg tcagcgccgc cacggaggcc gcggttaacg cttccatttc
    13441 gacaccggtt ttcccggtca ggcggcataa ggtttctata cgcacccgat tgtgctccgg
    13501 ctcggcctgt aaattgactt caactttgct gagcatcagc ggatgacaca gcgggatcag
    13561 atcccaggtg cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc
    13621 tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc
    13681 ttccgcccgc gcttcacgca cggtttccgc tttggcggag acatccacca tgtgcgcttc
    13741 gccagcggcg ttgatatggg tcagttgcga catacttatt tcttcaaatg tggatggaaa
    13801 ttacacggac gcgtacgggc atccagctgc ggcgcgatga tattttccca tgcggtacgg
    13861 cacgctttgg tcgaacccgg catggcgaaa atcagcgttt tgttagcgac gcccgctacc
    13921 gcacgagatt gcaacgtgga agtgccaatc tcttcaaacg acaacatacg gaacacttca
    13981 ccaaaacctt caacttcacg gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct
    14041 tccgtcaggc cagtcccccc cgtaatcaac accacttgta catcgtcgct ggcgatccac
    14101 gcagatacct gagcgcgaat agcgtagcgg ttttctttca caatggcttt atcgacaacg
    14161 tgatggcccg cttcttgcgc cgaatcgcgc aaatagtgac cggaggtatc gtcttcttca
    14221 ccgcgacgat tagaaaccgt aagaatagca atacgggtcg ggataaattc agtgcttacc
    14281 tgactcatct gatctctcct tttgacgttt tagccgccaa tgtacgataa gttttgcgta
    14341 ataccggtgt tgttttgatg caggaaatgg gtctgtttct tctcccgcag cgccgctgaa
    14401 atacgcgctt ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg caggttaacg
    14461 ccgccttcac caaacaggca gagatggagt ttaccaatgg aggaaacgcg cagacggttg
    14521 caagtggcgc agaagtcttt ttcatacggc atgataaggc caatctctcc ggcgtaatcc
    14581 ggatgacaaa agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg gatccagccg
    14641 cgacgcagta gctcgtcacg cagaacctga ccagagatgt gatgcttacg gaaaagctcg
    14701 ctgccctcgc ccgtttccat cagttcgatg aaacgcagct ggatagggcg atgctggatc
    14761 cagttcagaa aggtgtcgag ctggtgatga ttcacatcac gcatcagcac ggtattgact
    14821 ttgaccttct caaaaccggc ctcaaatgca gcatcgatcc ctgccatcac ctgattgaat
    14881 ttatcctggc cggtaatggc atgaaactgg cgggcgtcca gactgtcgac gctgacgtta
    14941 atgccagtaa gtcccgcatc gcgccagttc gccacatcgc gttccagacg gtaaccattg
    15001 gtggtgaccg ctatctggcg aatagcgtcg ttttctcgca cagcggcgat gatatcggta
    15061 aagtcgcggc gtaaagacgg ctcaccgcct gtcaggcgca ctttttcggt gcccagactg
    15121 gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtca gaaagccttt attggtgacg
    15181 ccgctcggtt tgtagccatc cggcaggcag taggtgcaac gaaagttaca cacatcggta
    15241 atcgacaggc gcaagtagta aaacttacgc gcaaatgcat cagtcagttg tgaagccatg
    15301 tacacctttc cagatacggg aggcgaagtc atttcttcct tcgccctggt ggcaatcttt
    15361 acgagcattg tcacggccaa agcaccatat cagttgaccc aggtgcagag gctagagtgt
    15421 ttagtggtta agccgatact agcgtgtaaa tgagattttt accatccaca ctttcgctat
    15481 gtaatcatgt atatagcgtc ctgatcgtgc tatttcgcta cagaataagg gaaaatggcg
    15541 gttttacttt gatatacatc atcagggagg catagcgtgc atcgtccttt agaggtagaa
    15601 gaaaatagtt tgtatcctca atattggcag gttaattgct gtttccccgc aatttgcgct
    15661 actgtagcgc ggataatctg actccaggaa actatatgcg caatcgtacg ctggctgatc
    15721 ttgatcgtgt cgttgctctc ggcggagggc atggactggg acgcgttctc tcatcacttt
    15781 cgtctttggg ttctcgttta acgggtatcg tcaccaccac ggataatggt ggctcgacgg
    15841 ggcgtattcg ccgttcagaa ggcggcattg cctggggcga tatgcgcaac tgccttaacc
    15901 agctgataac tgaacccagc gtcgcttcag caatgtttga atatcgtttc ggcggtaatg
    15961 gcgaactttc cgggcacaac cttggaaacc tgatgttgaa ggcactggat caccttagcg
    16021 tgcgacctct tgaagcgatc aatctgattc gaaatctgct gaaagtggac gcacatttga
    16081 ttccgatgtc ggagcatcct gttgatctga tggcgattga cgatcagggg catgaagttt
    16141 acggcgaggt caatatcgac cagttaacta cgccgattca agagttattg ttaacgccta
    16201 atgtacccgc aacgcgtgag gcggttcacg ctatcaatga agcggatctc atcattattg
    16261 ggcctggcag tttttacacc agcctgatgc caattctgct gctgaaggaa atcgcgcagg
    16321 cattacgccg cacgccagcg ccgatggtct atatcggcaa tctggggcgt gagttgagtt
    16381 tacctgcggc caatttgaag ctggaaagca agctggcaat tatggagcag tatgttggta
    16441 aaaaggtcat tgatgcggtc atcgtcgggc caaaagtgga tgtctcggcg gtgaaagagc
    16501 ggattgtgat tcaggaggtg ctggaggcca gcgatattcc gtatcgtcat gaccgccagt
    16561 tgttacatag cgcactggaa aaggcgttac aggctttagg ttaacggaaa tccgaaggaa
    16621 aattccggct tcctaatgaa gacaaagtgc gcgttgttta tgacggatgc ggcgtgaacg
    16681 ccttatccgg cctacaaacc gcgctaattc aatgtattgc agaatcatgt aggcctgata
    16741 agcgtagcac atcaggcaat tttgcttttg tcatctgtct tcttcgttat cctgtcacga
    16801 agccgcgata aacaattcac gcagctgatg caactggtca cgaatttgtg ccgcttcttc
    16861 gaactccaga ttttgcgcgt gttgcatcat taacccttcc agctcatgga ttttctgttg
    16921 caacgcttta ggcgacatat ccatcggcac attatccggc tcaacaatcg gtcgcgattt
    16981 tcctctgccc ttcgctttgg ttttagcaat gttctgcccc aacgccagga tatcgaccac
    17041 tttcttgttc aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc
    17101 gcggcgacgt tcggtttcgc caatcgcttt cgccattgac ggggtgatct tatcgccgta
    17161 gagaatcgct ttaccgttaa cgttacgcgc cgcacgacca atagtctgga tcaacgaacg
    17221 ttcagaacgc aggaagcctt ctttgtcggc gtcgaggatc gccaccagcg aaacctccgg
    17281 catatccaga ccttcgcgca gtaagttgat ccccaccagc acgtcgaact cgcccagacg
    17341 caagtcgcgg ataatctcca tacgttcgac ggtgtcgata tctgagtgaa gataacgcac
    17401 gcgctcaccg tgttcttcga gatattcggt aagatcttcc gccattcgct tggtcagtgt
    17461 ggtgaccagt acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc
    17521 aacctgtgtc gccactggcc gcacttcgat aatcggatca agcaatccgg ttggacgcac
    17581 cacctgatcc accacatcgc cgccggattt ttccagctca taattacctg gcgtcgccga
    17641 aacatagatg gtttgcggcg ctaatgcttc gaactcttca aatttcagcg gacggttatc
    17701 cagcgctgat ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc
    17761 gcgatacatg ccgccaattt gtggaatggt gacgtgagat tcatcgacca ccagcagccc
    17821 atcggcaggc aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag
    17881 gaagcgcgag tagttttcaa tccccgaaca gtagcccagc tcgttcatca tctccagatc
    17941 aaactgggta cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaatagcac
    18001 tttgcgtctg gcggccagct cttctttgat ctcctccatc gcctgtacga tgcgctcgcg
    18061 cggtgtgacg tagtgcgttt tcgggtagat ggtaaaacgt ggaatggtgg aaacaatctg
    18121 cccggttagc gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg
    18181 aagtgcaatg tcatccgatt ctgccgggaa gatatcaatc acctcgccac gaacacggaa
    18241 agtaccacgc tggaatgctt gatcattacg agcgtattgc agctccgcca gtcggcgcag
    18301 aatcgcgcgc tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa
    18361 atcaggatcg cccagaccat aaatcgcgga aacagacgcg accacaacaa catcacgccg
    18421 ctccagcatc gctttggtgg cggacaaacg catctgttcg atatgttcgt ttaccgaggc
    18481 atctttctca atgaaggtgt cggaactcgg tacataggct tctggctgat agtagtcgta
    18541 gtaggagacg aaatattcca ccgcgttttc cgggaagaac tctttcattt cgccatacag
    18601 ctgggccgcc agcgttttgt tgggcgcaag taccatggta gggcgctgaa ggtcagcaat
    18661 gacattggca atggtgaagg ttttcccgga accagtcaca ccaagtaacg tctggtgcgc
    18721 caggccatct tccagcccct cttcgagacg tcgaatcgcc tctggctgat cgccagaagg
    18781 tttaaaagcg gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg
    18841 cgggcaggta tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa
    18901 aaaaacagtt catcaccata atatttctgt tacagcgtaa actccgctgt caatcatgag
    18961 taaaatttac tctgtggcga gataaaactc ggaccttacc gggttatccc caaagcaacg
    19021 gcttttttaa catttgtcaa gatgagtgat gtcagtttta tggcaagaga tgcctgttca
    19081 gtgactgcca ttgcaattat ctaaccagtt aaaaaataaa agatatttct ttgagccgtc
    19141 tttaacgcca attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc
    19201 taatacacat ggttatccac aggaatagtg gataactgtc tgcagcccct atcccccgcc
    19261 gcctgggcaa ttcccacatt cgccgcatga tgcggaagta aaattttttt cgtgattatt
    19321 ttagaattta attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac
    19381 caaatacagg atggctacaa caaggcaagg tttatgtact ttccggttgc cgcattttct
    19441 ggattttctg caagccaggg gctctctccc agcagcggcg cgggaatcat gcgggtgagc
    19501 gtggtcatat attcagcgtg acgttttccc ggaggcgtga catcgttcgc cacccaaccc
    19561 gccagagtca gcccggcgtg ttgtatcgcc tgtgccgtca acatcgcgtg attaatacag
    19621 ccaagtttca cgccaaccac cagtattacc ggcagttgtt cctgtgttac ccaatccgca
    19681 aaagtgaaag tgtcagaaag cggcgtaaac cagccgccag cgccttccac taacacccag
    19741 tcagcctgtt gttcaagcgc gcgtaatccg gagctcatta ccgatgattc tatcgacctg
    19801 ccctcttgcg cgctgatgat gtgcggtgag gtgggttctg cgaaggtgta aggatttacc
    19861 gttgcgtaat ccagctgcag gctgctgttg cgctgtaacg ccagcgcgtc actattacgt
    19921 aaaccctccg gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgatag
    19981 cctgctgcct ttgcggcttg taaaagtgca caactggcga cagttttccc cacttcggta
    20041 tccgttccgg tgacaaaata acgtttactc acgaacaatc actcccaaaa aaagatgata
    20101 cgtcagagga tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaactgtga
    20161 acgcgttaat attcgctggt cgcgcccttc atgaagatgc gtggcaccga tgcctttcag
    20221 cgaacgcatg gcactgagcg catcatcaaa ccatagtgtg atgggctgaa tacgatgttg
    20281 ataatgcacg ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc gattagcatg
    20341 cgggcgctcg tccaccgcct gccacgcctg atgcagttcg ggtaacgatc cctgcaccag
    20401 cgtggtaaac gcgaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt
    20461 tgataaatta ccgcaccact gcaccgcgag attgctccat gcaagatcga acgtcgcagt
    20521 cgctaacggc agggattcga tatctcccgc cagataatgg tctgcagcat ctttctggcg
    20581 ggcttgcgca agcattggcg gcgagagatc taacgccgtc acctgcgcgt gacgctctcg
    20641 ccagtggcgg ctcatccagc caggcccaca ccccgcgtcc agtacgtggg ggtatttacg
    20701 ctgtgggagc attgccagta agacatcagc actctggcgc tgtaactctg cgtgttgctc
    20761 atagtgcgcg gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat
    20821 gcagcacctc cagcagacgg tcgatatcct gcatttcatg cgcggcggtt agcgttaagc
    20881 gcagtcgcgc agtaccagag ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt
    20941 gctggcgcag tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct
    21001 ggatggcgct gcatgaatca gcaagcgtaa acggcaaatc ctgtactccg gcacgaaaac
    21061 gcgtaatgag tgccgccagt ttttcgcgcc gtgcatcacc ctcatcactg cgaatgaccg
    21121 ccagtgacgc acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataagat
    21181 ggcgggcgaa ttgcagcaga taatccgcca ccgtactgga gcaaagcacc gcagccccgc
    21241 tgacgccaaa tcctttgcca aaagtcacta ccagcaattc tggttttacc ttttgcaacc
    21301 agcaggtgcc gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca
    21361 accagccatt gtgctgttgc gttacctgct ggatttccgc cagtggcgca ctatcgccgt
    21421 ccatgctgaa cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca
    21481 gtcgcgccag atgagtgaca tcgttatgaa caaaacggcg aagctgcgac gggcttaaac
    21541 tggcagcctc cagcaatgag gcatggctaa gccggtcagc gacaatgcgg tcctcttttg
    21601 ccatcatcgc ggcaatgacc gcctgattag cggcaaaacc agagataaat agcagtgccc
    21661 gcgaatagcc cagccactcg gccagttctt cttccagcgc ctgatgcgca acgctataac
    21721 cgctgacgtg accggaaccg ccgctaccga cgccaaattg ctccgcaccc tgcttccagg
    21781 cacggataat ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat
    21841 actggcgatc gtccgccacc agccagcgtc cggctccttg cgccactgga taacggcgac
    21901 gcagggcatc ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc tgccagctca
    21961 tagtgctgcc gcgttgtaat attcgtcagt gtccggggtc atcagtgcct gctccagacg
    22021 ctgctgttgt tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt
    22081 gcgaaacagt tgcaggtctt tatcttcttc cggattgggc gtggttagca gtttgcaacc
    22141 gtagaaaatc gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt cgttcatctg
    22201 ctcgcgtccg gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg ctacggcaat
    22261 ggtgcgaata aaatcaaagg catcgacatc atcgttatct gccagtggag tacctttcac
    22321 cttcaccagc atgttgattg gcacgctttc cggcggcgtc ggcaggtttg ccagttgcag
    22381 taataatccg gcgcgatctt ttaccgtttc gcctaaaccc acaatgccac ctgagcagac
    22441 tttgatcccg gcatcgcgca ctttttccag cgtatcaagg cgttcctgat aagtacgtgt
    22501 ggtgatgata ttgccgtaaa actccggcga ggtgtcgagg ttatggttgt agtaatccag
    22561 cccggcattc gcaaggcgct gcgcctgaga ttcactcaac gtacccagcg tcatacacgc
    22621 ctccagcccc atcgctttta ctccctgcac catttgttcc aggtatgaca tatcgcgttc
    22681 gtggggattc ttccacgccg cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt
    22741 gcgcgccgac tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt
    22801 gtagcgcgag ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga
    22861 cagcaaggtg ctgacctgca cctgacgagg atcgaaatgc tggcgatgca cctgctgcgc
    22921 ttcaaacagc agatccagca acggtttttc aaataattct gtgacttgcg acaatgtcca
    22981 gcgtgggcgg tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta
    23041 gacttgtaaa cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc
    23101 ttgcctttga ccaacgccat atctggcacc catacacatc catgacctcc ccgctaccgg
    23161 tttatccggt ggcgagcgcc gaaggttgcg agctaatttt gtctgacggc agacgcctgg
    23221 ttgacggtat gtcgtcctgg tgggcggcga tccacggtta caatcacccg cagcttaatg
    23281 cggcgatgaa gtcgcaaatt gatgccatgt cgcatgtgat gtttggcggt atcacccatg
    23341 cgccagccat tgagctgtgc cgcaaactgg tggcaatgac gccgcaaccg ctggaatgcg
    23401 tttttctcgc ggactccggt tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt
    23461 actggcaagc caaaggcgaa gcgcgccagc attttctgac cttccgcaat ggttatcatg
    23521 gcgatacctt tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga
    23581 aaggctatct gccagaaaac ttgtttgctc ccgccccgca aagccgcatg gatggtgaat
    23641 gggatgagcg cgatatggtg ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg
    23701 cagcggtgat cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg
    23761 aatggttaaa acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg
    23821 agatcgccac cggatttggt cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg
    23881 cgccggacat tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca
    23941 cactcaccac gcgcgaggtt gcagaaacca tcagtaacgg cgaagccggc tgctttatgc
    24001 acgggccaac ttttatgggc aatccgctgg cctgtgcggc tgcaaacgcc agcctggcga
    24061 ttctcgaatc tggcgactgg cagcaacagg tggcggctat tgaagtacag ctgcgcgagc
    24121 aacttgcccc cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg ggggccattg
    24181 gcgtagtcga aacaactcgt ccggtgaata tggcggcatt gcaaaaattc tttgtcgaac
    24241 agggtgtctg gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc
    24301 tcccgcaaca gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat
    24361 ttttttgcca ataacgggaa gtcagcgtga gagtttctgg ctacactttc tgcaaactag
    24421 aaaggagggt ttatgaaact catcagtaac gatctgcgcg atggcgataa gttgccgcat
    24481 cgtcatgtct ttaacggcat gggttacgat ggcgataata tttcaccgca tctggcgtgg
    24541 gatgatgttc ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca
    24601 accggctccg gctggtggca ctgggtagtt gttaatttac ccgctgatac ccgcgtatta
    24661 ccgcaagggt ttggctctgg tctggtagca atgccagacg gcgttttgca gacgcgtacc
    24721 gattttggta aaaccggata cgatggcgca gcgccgccga aaggcgaaac ccatcgctac
    24781 atttttaccg ttcacgcgct ggatgtagag cgtattgatg tcgatgaagg agccagcggc
    24841 gcgatggtcg ggtttaacgt tcatttccac tctctggcaa gcgcatcgat taccgcgatg
    24901 tttagttaat cactctgcca gatggcgcaa tgctatctgg tatcacttaa aggtattaaa
    24961 aacaactttt tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa aagctgaacg
    25021 cgaaacagca aaacccaata atatcaatgt attacaatgc atttagtcta aaaaatagac
    25081 tgcataatgc tacaaaacac aacatatcca gtcactatga atcaactact tagatggtat
    25141 tagtgacctg taacagagca ttagcgcaag gtgatttttg tcttcttgcg ctaatttttt
    25201 gtcaaacaca ttacgtcgtt tgctgaattt acaaccatgc tcagtatctc tataagcgca
    25261 gagaaatgat gcagtggtgg gtgggctggc ttgatgaaaa ggtggggtac tccacctgaa
    25321 ccactatcgg aatagcacaa agccttgcaa tccagtgcaa agttttgtgt gcctccgttt
    25381 gccccaccaa tatatattca gcacaagaaa cacagctaaa gcaatggatg cagtcttacc
    25441 tgttggaatc ccagactcac tgtattgtca tcatgaataa gagctgagtc gtgcgggtag
    25501 gaggttctac ggggcatccc aatctcttcg tggaatttat tttctgaaag cacctcctct
    25561 gaaaaagccc ccaacccaac agtcctggct tcttcgagta aaagtggaga gtatttttgt
    25621 gattctgctc gggaactctc aactttcaaa cgctccccat ttaaattgtg gaaaacatgg
    25681 agcaactcat gaaaaacaat agtcgcatga aagtccacca cgctaatccc ctccccacag
    25741 ggatattcaa ctgcattcag attacagtga aaatcggaac cagtcccccg atggttctca
    25801 gcatctatat ctctatgtgc cataactcct agtctggaag agttgaggtg tattaccact
    25861 gtttctgatt taagtcggga tattgattca atagcattca ggagtacacg ccctgtctct
    25921 gtagagccaa tcttatcgag tgctgcttca atctgctgat atatattatg atcagtcccc
    25981 tggaaacgaa gttgcggatt attttttatc cattgaacgc tcgatgcttt tacaagagac
    26041 tcgatatctg tatctgacat tgagctcgac cttgatgact gatgtaatac caagttgagg
    26101 gacgtagggc gcatcaggac tcctgagaat gctacttatt agcagcctgt ttaccactct
    26161 gtgaaaatga tttttttaaa agtgaccgtc aaggcggtca ccaaacccga taataaagcg
    26221 gatcatagcc atttgccagt cctcagcgac attgcccatt tccgtgatcc gccaagaatt
    26281 gccaaccaca caatttgtta ccaactccat ccatgcaaca aatccactac actggataaa
    26341 attactaaat tttacttaat accacactaa taagatcttg ctttccgcca tgataagatt
    26401 gcattatttg actttcactc catgaacggt cggaaacatt ataagatgat atatctattg
    26461 gattaaactc attcttcgcg cggtcatata agacatttgt ctctgttgta tcgacaaaaa
    26521 gaattccttt ttgctccagt ctatcaaaca tatcgtaaat agcatgctca gcctgtgctg
    26581 gcaaggacga aatatttaaa agcgattctc catttatttt atccattcta ataccaataa
    26641 tatcaccatt attgccatat attttttctg cactcccggc accataatat tggttgaagc
    26701 aacgaacttc gcttgtcacc tcttcattgc tttgagatgt agtaaacatc ttcaggactt
    26761 ttgttgcatc ctcagcatct tcatatacta cagcgttccc ccctttgcca ataacattac
    26821 caggtactga caacctgtta tagtccacac tcggtaactc tggcggtgca taatcaacag
    26881 gaggtaaatc aggtctgttc gaatgaacaa tgcctctttc catagcagcg ctcactggtg
    26941 atgcattcag catgacctca attttcctgc taatctcccc cttaggccat cccagcctgt
    27001 gcaacagatt agtaaaacaa ccactatgac tttctcttgt aacgcaaaac ttattatcgg
    27061 tggcaacaac acgatatgtt ctgttgccaa cctttacttg cgccccatta tcagaatgaa
    27121 cggcagcatc ccttacagag gataaaacac gattatcagg cgaagtcagg tttctggtta
    27181 aagaattcca tgaacatccc aaatttacag aatatggcga taacatacat ttcaaccttc
    27241 aaaataaacc catctaaatt tccttaacaa acatcccccc taacatggca acaaaaatta
    27301 gaaatgaact cttgcttttt gtatctggca actaattcat ccttccagca tcccatcaaa
    27361 cgaacgttta cgaactataa cgcaggtatt accggtcact cgatatctgg agatacagac
    27421 gtatcaacat gattgagcgg catacgatac tgcttccatg ccgccagtaa cgatgtctgc
    27481 tcatccgtcg caatatctaa atctgcagca tcctgaagtg gtgcgatata ctcactgatt
    27541 acaagtacca gaccgataga ttaaaaaaaa cacaataac
//
LOCUS       DAZFTN010000062        27502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFTN010000062 DAZFTN010000000
VERSION     DAZFTN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..386)
                     /locus_tag="ACHKV2_002909"
     CDS             complement(<1..386)
                     /locus_tag="ACHKV2_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFO6940765.1"
                     /translation="MKNNNVPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYF
                     CVVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMR
                     KHWDVLLSLISKAINNDEIIVMHSHL"
     gene            complement(383..679)
                     /locus_tag="ACHKV2_002910"
     CDS             complement(383..679)
                     /locus_tag="ACHKV2_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HFO6940766.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILVSPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
     gene            complement(846..1100)
                     /locus_tag="ACHKV2_002911"
     CDS             complement(846..1100)
                     /locus_tag="ACHKV2_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO6940767.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            complement(1262..1813)
                     /locus_tag="ACHKV2_002912"
     CDS             complement(1262..1813)
                     /locus_tag="ACHKV2_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940768.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNGSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            complement(1986..2669)
                     /locus_tag="ACHKV2_002913"
     CDS             complement(1986..2669)
                     /locus_tag="ACHKV2_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFO6940769.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            complement(2922..3455)
                     /locus_tag="ACHKV2_002914"
     CDS             complement(2922..3455)
                     /locus_tag="ACHKV2_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO6940770.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRRCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            complement(3897..4184)
                     /locus_tag="ACHKV2_002915"
     CDS             complement(3897..4184)
                     /locus_tag="ACHKV2_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6940771.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            complement(4222..4380)
                     /locus_tag="ACHKV2_002916"
                     /pseudo
     CDS             complement(4222..4380)
                     /locus_tag="ACHKV2_002916"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            5338..6369
                     /gene="repA"
                     /locus_tag="ACHKV2_002917"
     CDS             5338..6369
                     /gene="repA"
                     /locus_tag="ACHKV2_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO6940772.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPARYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            7277..7561
                     /locus_tag="ACHKV2_002918"
     CDS             7277..7561
                     /locus_tag="ACHKV2_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO6940773.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            7561..7836
                     /locus_tag="ACHKV2_002919"
     CDS             7561..7836
                     /locus_tag="ACHKV2_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6940774.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNDQTIYVLRLWHTRENR"
     gene            7942..8145
                     /locus_tag="ACHKV2_002920"
                     /pseudo
     CDS             7942..8145
                     /locus_tag="ACHKV2_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8415..8681)
                     /locus_tag="ACHKV2_002921"
     CDS             complement(8415..8681)
                     /locus_tag="ACHKV2_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940775.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQCLLLTRNISGSVMKK"
     gene            complement(8681..9175)
                     /locus_tag="ACHKV2_002922"
     CDS             complement(8681..9175)
                     /locus_tag="ACHKV2_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940776.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLKLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKHILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(9231..9833)
                     /locus_tag="ACHKV2_002923"
     CDS             complement(9231..9833)
                     /locus_tag="ACHKV2_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6940777.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            10187..11602
                     /gene="yagA"
                     /locus_tag="ACHKV2_002924"
     CDS             10187..11602
                     /gene="yagA"
                     /locus_tag="ACHKV2_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO6940778.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMEYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFRGLTLSGTKTHVKQR
                     FLPLTPLLMVSDVLWSPVLPA"
     gene            complement(11526..14531)
                     /locus_tag="ACHKV2_002925"
     CDS             complement(11526..14531)
                     /locus_tag="ACHKV2_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFO6940779.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            14695..15252
                     /locus_tag="ACHKV2_002926"
     CDS             14695..15252
                     /locus_tag="ACHKV2_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6940780.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            15435..16295
                     /locus_tag="ACHKV2_002927"
     CDS             15435..16295
                     /locus_tag="ACHKV2_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO6940781.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            16767..18647
                     /locus_tag="ACHKV2_002928"
     CDS             16767..18647
                     /locus_tag="ACHKV2_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO6940782.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLSHSGLDYKRNILNDQNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLSGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFW
                     GI"
     gene            complement(18669..19004)
                     /locus_tag="ACHKV2_002929"
     CDS             complement(18669..19004)
                     /locus_tag="ACHKV2_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HFO6940783.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            19133..19480
                     /locus_tag="ACHKV2_002930"
     CDS             19133..19480
                     /locus_tag="ACHKV2_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO6940784.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCVMWFTYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(19500..20090)
                     /locus_tag="ACHKV2_002931"
     CDS             complement(19500..20090)
                     /locus_tag="ACHKV2_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940785.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(20090..20344)
                     /locus_tag="ACHKV2_002932"
     CDS             complement(20090..20344)
                     /locus_tag="ACHKV2_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940786.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDNQSARCISELKASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            20913..21326
                     /locus_tag="ACHKV2_002933"
     CDS             20913..21326
                     /locus_tag="ACHKV2_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940787.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            21328..22107
                     /locus_tag="ACHKV2_002934"
     CDS             21328..22107
                     /locus_tag="ACHKV2_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6940788.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            22287..22931
                     /locus_tag="ACHKV2_002935"
     CDS             22287..22931
                     /locus_tag="ACHKV2_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO6940789.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            23018..23326
                     /locus_tag="ACHKV2_002936"
     CDS             23018..23326
                     /locus_tag="ACHKV2_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO6940790.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            23739..24719
                     /locus_tag="ACHKV2_002937"
     CDS             23739..24719
                     /locus_tag="ACHKV2_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6940791.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            24712..25128
                     /locus_tag="ACHKV2_002938"
     CDS             24712..25128
                     /locus_tag="ACHKV2_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6940792.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(25130..26404)
                     /locus_tag="ACHKV2_002939"
     CDS             complement(25130..26404)
                     /locus_tag="ACHKV2_002939"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO6940793.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(26404..26841)
                     /gene="umuD"
                     /locus_tag="ACHKV2_002940"
     CDS             complement(26404..26841)
                     /gene="umuD"
                     /locus_tag="ACHKV2_002940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6940794.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(26838..27086)
                     /locus_tag="ACHKV2_002941"
     CDS             complement(26838..27086)
                     /locus_tag="ACHKV2_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6940795.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
BASE COUNT         7160 a         6463 c         6773 g         7106 t
ORIGIN      
        1 aagagatgtg aatgcatgac aatgatttca tcattattaa ttgcttttga tatcagagaa
       61 agcaatacat cccagtgttt cctcattgac ttcgggcaac gtgtgtcagc ataggggcta
      121 agatgtatag ttgatttctt gcttcccttt ttcttcttca caaagaatgt gaagtcgtat
      181 ttagatgcag accttacaat aagaagtaca catctgaaaa gtagtgggaa aatgcaaatc
      241 acaaccacca cacagaaata taacatataa ggcaatgata agttgtcagt gtatggataa
      301 cgtaagtatt gccatggggt agtgatgtac agacaaagtg tacaccaaag caaccataga
      361 tataatggta cgttattatt tttcatatta gttgactttt aatactgttt tctatcacca
      421 catttttatg tgtctcattt ggcacttcgt actgttggaa atattgttgg cggcggatgc
      481 gtgtctgttc actgctttct attaacaccg ccatggcgat tgcaaagtat atgcaaatca
      541 ggaaaatcca gaacgcgttt ggtatcttcc aaaaaagtgt ggacagtttg ttatgtggtg
      601 aaactaaaat cggagaaagg attccacaga caatcacaat cgcgatggtt aatttaacta
      661 gaaagaacat ggtaaacatc gttaatctcc atgataaaag ggagagaggt aataaagatg
      721 gcaacaaacg gctaaattaa aatcgttaaa aggtgtacac agacaatcgc aatcgcgatg
      781 gttaatttaa ctaaaaagaa cacggccccc acctgttttg acgaggtctt ttgtttttgt
      841 tgtgtttacg acagccagtc ctgtagatgc tgatggcggg agctgctgcc cttgtgccag
      901 cggcagtggc tgccgggagc acgctccagt cggaacagaa atcggttgtc gcgtagtacc
      961 accacgagac agttatccgg cgtgcagaaa cgtcgggcaa gctcatcggg gtctgtcggt
     1021 gttttcgttg aaaacagcct gcgtttgcag tgtgcgtcga cgaccagtat ttgcagtcgt
     1081 ccgggatgtt gctgtggcat caaagactcc ttccggaacg ggaaaagaag cagctgtacc
     1141 gtcataactg cacgtaccgg ttgcgaaaaa acggcacaag cgagtagtga cagaaagaat
     1201 gtcggaaacg gcaggaaggg cggtacaggc tgcgaagacg ggaaacgggt tgtgttggga
     1261 attaacgcac gtgaggtgtg atgagaacga cgcgcttttc gccagctccg tctccggtga
     1321 atccattcac gacaagggac tgttccggtc tgagagaaat gctttgttca acagtctgtt
     1381 ttcgtttctg gtctgtggtc gagagtcgca gagtgatatt tccgggcgca ccgtgaatat
     1441 ccggtgatac ctgcattctg acaccgggag agacatctgc agtgaaggta tccggaccta
     1501 cggacgttac cagtgattca acacagggcg tattctgtat catcccgtgg agcgtttcag
     1561 gtgtcagcgt caggctgcca ttaaaaaatg cactgcgtga aatactcccc tgagcaccgg
     1621 caaacaggtc cggatggcgc tcattaagaa ggcttccgta tttttccgcc aggcagattg
     1681 ggttgctggc cgttgtgtct gttcgcacgg tatcgaccgt gaatgtcacc tgcgggtgat
     1741 gttccctgtg gcctgtgcag ccggacagca gtgcggcggc tatcgtcatc cagagtagtg
     1801 gttgtttttt catgctgtta atttcctctc agggtaaaaa gaaagactcg caccgtcatg
     1861 tcgatggggg ccgcttgcga aaaaatggca caagcgagta gtgacagaaa gaatgtcgga
     1921 aacggcagga agggcggtac aggctgcgaa gactggaaac ggggtatgcc ggggattaca
     1981 catcctcagt gtaatgtgat agtcgtctgc tcctgtgttg caccccagac atcttctggc
     2041 aggctgggca gcccgataaa tagggtgtga aagagggttt ctctgcgttc gatcccgaat
     2101 tgatgttcgt atcctctcac cacctgaaat ccttcgtcgg agaatattaa ctctgccgtt
     2161 tccagaagct gcatagtttt gcggtgtagt ggagtgatca gctgcaacgt aactggtgcc
     2221 ctcatatcgc ctgtcggtag aaaacactct tcggtgcgac ttagttgtag cagatgacgt
     2281 ttcccggaac aggcccgttc aatggattca ttgaacaccc tctcaggcca tgggcagaca
     2341 tatgtgcgcc agcctgcttt ctgtaattta cccagtgcag cccgaaaacg aaacaacgga
     2401 cattccccga cagtggcaga gctgagaaag agcgtattca gcgttccgat gatgggaata
     2461 ttgcgatctt tttcggtcaa tgcttttaac gccatgattt cgccagggag actacggaac
     2521 tcaggatgtc tgctggcacc agaaagccat tcaagcaggt aatgccgtaa tccggtttca
     2581 ctggttatat tgtggttttc ctgtgccgtt ttcagggcat tcagtgcaca ccagagcaac
     2641 tggcgcacgg gatgttctgt cgtggtcatg cgaatgtcct actgtgagat gaaaagaaga
     2701 ccggtatccg ctctctccgc cacacgtgat ggcacgggcc ggcctttgat aaaacgaatc
     2761 aatactctgg ggtgaagcct tttatttctt tcgccattaa gaaatacacg attccatcaa
     2821 aagacagggg gagtccatgg tggggatggc ttcgtgcagg cttcactcca gaatattctc
     2881 tgaattgaaa aaagcccggc ccgaaggccg ggaaatactg gctatgggat gtttacaagg
     2941 cgctccataa agccgtgatg ttcggcattt gataccagtt ccaggagcag ggatatcctc
     3001 gactcaggct ggagagtatt ttccattctg aggagatact gatattgtgc ttttgtcagc
     3061 catatgctgg aagccgcatt cagctcttcc ctggctcccg gatttagcac agggtccggg
     3121 taaatcttca ttaatccatc aacaatatct ttattgacgg gttttatttc accgccaaac
     3181 ttcacgaaat caatgaaggc gctatggcga cgtaatgccg atactggttg aatgtcaaaa
     3241 ttagccagaa taaaaaagta ccctggaaaa atagcgaata ttctttcacg gtaacagcgc
     3301 ctggagtctg cccgtcttat ctttcttgtt attaatggag tccatggtac gacgttttgc
     3361 tcattcagcc atgagaataa agattctctg tttttgccgg ctgtattata ttgcgcaaga
     3421 taccagttcc ggttattcag gtgttctata ttcacgaagt tcaacgctcc gcgcaaaacc
     3481 gatccatgat ggacgatttt gcgatgtcag gcgaatatgc taaatcatat gtctgaagca
     3541 gtgtccagcc catatctgga taaactgcag ttctgacagg catataacca gcagactcta
     3601 gtacgttctg tatcggtgat ttggcgaact cacagataaa gaacggctaa gcactaatat
     3661 cagcaataca gtggcacagt attgtgatac gcgaaataaa acatcagcac gtcagttcag
     3721 agtaagatcg ccatatatcg ggcatgagcc agatacaaat aactgggaca ccgtctgaag
     3781 tgatttgctt tattaaagta acttgtgctt ggtgtttatg tttatcaatt tgggatcgcc
     3841 tgtaaagtgc aaaatgcaat ttccatgaaa gtgtcccctt taatggggac acctgttcac
     3901 attttttcgt tttctttagc tgtattgcta atcatgaaaa cgaaagttcg catcagcaca
     3961 gcagagcgaa gtgcatcata tccttccaga tttaaattct tcaggcttcg cttcaggtag
     4021 gttcgtaccg tttcgggaga aagccgacag ttctgagcaa tggcgtcgta aggaacaccc
     4081 attgcgtaaa aaacgcagac tttaagctgg tttgttgata gctcgggaaa catgccttcc
     4141 aacagagtga tggccgggag tttctgtatc agttgaggtg acatagcaaa ggtctccgat
     4201 gacaactaaa aacgctatca ccgagttcct acgctcattg gtgatagccc agacgggggt
     4261 aggaataccg gcgtcatcgg aaaccggcca ggctaatgcc tgccccgcct gagctaccat
     4321 agatgacgac aagaaataca agttcctgtc tttatggtat ggcaaaaaac gacgccaaat
     4381 gaagaaatta acttctgcgc cgacgatgac tacgcgggtt cctacgcccg atcacagttt
     4441 tgcctgtgac cagtaaaggg tacttagtat aacgcaccga gtcaactaac agcttaaaaa
     4501 acatacaaaa aagcgtcaaa acttaacgct ccgcgccgaa gcgatccagg catgatgctt
     4561 ctgctttgtg gcgttcgaac atgctggtcc atatgcacga aacggtgtct gaccctcgtc
     4621 cagacaatcc gcagttctga cgaacatatg aacaaatatg ctcttgtaga ggctgttgtt
     4681 acacaattgg cgggttgtgt aacaaatggt tttatcatct aagaattatt ctatttacga
     4741 tcgaatgatc tacaaaatga tttttcaaag gttttttaga tcgtttaatc gcattaaaac
     4801 agaatattat tctgttaggt ggatcggttg taagataaat gatcgtgagt aggacaaaag
     4861 tcgatctttt aatccaatag aaggatctaa agcagaaaaa tctgctttta aaaatagatt
     4921 ttattttttt gacgcaggtc aagattaact tattagaggt atcagtgagg aggcactgga
     4981 agagaagaga tcgttgtaat gcttttcaaa ttaacgtaaa gcgggtatat ttcggctgtt
     5041 attagctgtg cagagggtgg cactctgtgg agcaaagcgg cgaaagtcgg acggcagaat
     5101 gcgccataag gcattcagga gagatggcat gtacgggcag taagtcagaa gactgaagat
     5161 gttccggaag ccataaaagg aaaaccccca ctatctttct tacgaacttg gcggaacgac
     5221 gaaagatagt gggggcctca cagaatacgg gtaaagtata atgaaaccgt accagagatt
     5281 caaccctgtg cagtgtataa atacacggca caatcgctcc gccataagcg acagcttgtg
     5341 gcaggtctga agaatactcc atataacgca gtacactgga gtcagttagc acccgaagag
     5401 cagatccgtt tctgggaaga ctatgaagcg ggaagggcga ccactttcct ggttgaaccg
     5461 gaaaggaagc gcacgaagcg ccgtcgcggt gaacactcca ccaaacccaa atgcgaaaat
     5521 ccgtcctggt atcgtcctgc gcgctataag gcgctgagcg ggcagctcgg gcacgcctac
     5581 aaccgtctgg tgaaaaagga cccggtgacc ggcgagcaga gcctgcgcat gcacatgtct
     5641 ctgcatcctt tttacgtgca gaaacgaacg tatgccggtc gcaaatatgc tttccgtccg
     5701 gaaaaacaac gcctcctcga tgccatctgg ccggttctgg tcagcttcag tgatgcgggc
     5761 acacataccg taggcatgag tgtttcccgt ctggcccgag aaatcagccc gaaagacagc
     5821 aaggggaagg ttattccgga actggaagtg acggtctccc gcctttcccg tttgctggcc
     5881 gaacaggtac gttttggtgt gctgggtgtt tcagaggaaa ccctgtggga ccgtgaaacc
     5941 cgccagcgtc tgccacgtta cgtctggata acaccggcag gctggcagat gctgggcgtc
     6001 gacatggtaa aacttcacga acagcagcag aaacgactgc gtgaaagtga aatccgccag
     6061 cagctcattc gggaaggtgt tctgcgtgag gatgaagata tctccgtaca tgcggccaga
     6121 aaacgctggt atctgcagcg cagtcaggat gcgctgaaac accgtcgtgc gaaagcagca
     6181 gccagtaagc gcgccagacg cctgaagaaa ctgcctgccg accagcagat tcatgagatg
     6241 gcagagtatc tcaggaagcg tctgcctccg gatgaagcct atttttgttc cgatgaccat
     6301 ctgaagcgaa tggccatcag ggagttgcgt cagcttgaac tgacgctggc tgccccgcca
     6361 ccgcactaga cagcaccatt ccctcagcac tgaatcatca ccagcccctc cggggctttc
     6421 ggcgctggtt ccgctcagcc caaaatccgc agtaatcacc ttaaatcccc tcagaggggc
     6481 atatctgccc ataaaaccac gcatcagtca tcagaacatg gccacgtcgt ttcagttatc
     6541 cacataaatc cgcaaataaa ggactttaag aagctgcaaa cctgaaacag caaacctgca
     6601 atatagtctt aaccccatta cttaatcccc tgcgttgctt cgccgcaggg aaagtcttta
     6661 tctctgaaac cactgtgaac aaatacaaag aggccttcgc ttgcagcggc caaggccgcg
     6721 ccgctcagaa tctaaaagca cctcccacgc tgacgcgcgg gccccgaacc tcaccgttct
     6781 gaaaccacaa caaaaaaaca tcaggaataa aaacaccaca caaacgcagc accgtgccca
     6841 cccctcataa ctgaaaagcg aggccgcccc cgcccaaagg gcgggaacaa catcgctttt
     6901 aattatgaat gttgtaacta catctcatca tggctgtcag tgttctggct ggaagtatcg
     6961 agtacacgct cgtaagcggc cctgacggcc cgctaacgcg gagatacgcc ccggcttcgg
     7021 gtaaaccctc gtcgggacca ctccgaccgc gcacagaaac tctctcatgg ctgaaagcag
     7081 gtatggtctg gcagggctgg ggatgggtaa ggtgaaatct atcaatccgt accggcttac
     7141 gccgggcttc ggcggtttca cacctgtgct acatgtaacc accgtatgcc gccttacatg
     7201 cgctgacgcg cattatttgc cttgttccgt ctgaaagtaa tcactatgat taaatatgat
     7261 taacaactaa tcggatatgc aaatgaaaaa caataccgca caggcaacaa aagtaattac
     7321 cgcgcatgtg ccattaccta tggctgataa agtcgaccag atggccgcca ggctggaacg
     7381 ctcccggggc tgggttatca aacaggcgct ttctgcatgg cttgcccagg aggaggagcg
     7441 taatcgcctg acgctggaag ccctggacga tgtgacatcc ggacaggtta tcgaccatca
     7501 ggctgtacag gcctgggcgg acagcctcag tactgacaat ccgttaccgg tgccacgctg
     7561 atggaactga agtggaccag taaggcgctt tctgatttgg cgcggttata tgattttctg
     7621 gtgctggcca gtaaacctgc tgccgccaga acggtacagt ccctgacaca ggcaccggtc
     7681 attctgttaa ctcatccacg tatgggagaa cagttgtttc agtttgaacc cagggaggtc
     7741 agacggattt ttgctggcga gtacgaaatc cgttacgaac ttaatgacca gactatttat
     7801 gtattgcgcc tgtggcacac acgagaaaac aggtagattg tgtgtgggga caacttaaca
     7861 tagcctgcag atattaattc tggaacatta ttatcacgcc agatattttg cattccatta
     7921 aataatcttg taggagataa aatgaattac gatgaaataa cgaaaattac cgctgaacgt
     7981 attagcgact acatgactga agcagttaat acagactcta ttgccgttgc ggaaatgttt
     8041 cataacgctg catggggagt gcgaacattg tggtttgaac tcgtcacaaa aatagatatg
     8101 gatatgcata aaaagaacgg gtatgcgagt tatgatctta gaaggaaaat cgaaatgcag
     8161 catgaagagt ttcagaaaat gacagagcgc gagcaagtgc ctttattgaa atcgccataa
     8221 taatagaaac agtacaagta cattgctgta ttgtgttttc cttgaatatt gaatcgcggt
     8281 gcccatgtac tacgattcct ttcgtgtcat ttatcattgc acactttccc tgactgagcg
     8341 taaaacactg actactttcc cctcaaactc ttggacggag aatgcaaaat tggtctgttc
     8401 cagcatagaa tatgttactt tttcatgacc gaaccagaaa tattgcgcgt caataagaga
     8461 cattgtgtaa actcagtcct tttatctggc aggttaaccc ctatcaatct taggatgaaa
     8521 ccacgccatc ctctgtttgc ctggttatta atttcataaa gacgattgtt ttcctctcgt
     8581 agttttgtcc actctaaaca aagtgttata tcatccagtt ctgtgatgtg tttcactttt
     8641 ccattgacag ttacagtgtt tattccattt cctgtgatca tcatcttatg ccttttttat
     8701 cttcatttgg gaccgtgctt ttcttcggag tatcactatt cgttaactgc tgtttatcaa
     8761 taaaattcct gatgttatca agtgcttcct ttgtttttag aatgtgcttg atatccattt
     8821 catcccgccc ggtcattctt tttccggaca aaagacgctc aagaagctcc tgtttcgcat
     8881 caggagaatc tttggcgaga tccatcagtt taagcaaatc ttcacgatcg agaggaaaga
     8941 agtattttac aaaatacttg aaaagcgaaa gaaagtagta tgctgtccat gcaatacaga
     9001 aaccgattct gagaaactca ttgtcaacta tacagaaata caagtataca atcaggagaa
     9061 taggtaaaat ccataatact atagctaaaa aaatatatac ggggatcagg cgtctgtatg
     9121 cccgttcatg tttattaagg atgaataaaa tcttctccgc ttctgaatca cacataacat
     9181 tctcttccca ttttttctga tacgtaatgt ccacctgtta tcaggagtgt ttattgccca
     9241 gaaatattct cctgctgtgc ctgcgtcctg agccactttt gttctaatgt cgccagtctg
     9301 cgaccggcac tctgtgcttc agagtctgag acctgccctt tggggtgacc atccaggcca
     9361 taacgtggga cgccggcgac agctgtccgc tgcagatata cgcggcggga tgtgatgcag
     9421 gacactgcac agagccattc gtccatggtc agttctgtct ccgggtgttc tgccagaaat
     9481 gccgaaatat cattgcttat gccaattttc aggggaataa atccaccacc ttcagcacga
     9541 agatgaggaa acagggcctt tattttttcc acgcactcat gagtatactc acgggtccag
     9601 tgggagggac gtggaaaacg gtgtcttttc tttttcttct tcctggctgg cgcttcgggg
     9661 atatgtgttt cgacggcaga tgaatttatt ccggcggggg ctgattctgc cgtctgttca
     9721 gtaaatacag gtgcgataat atctgttttt tcggataagg acggtggcga aaaagttcga
     9781 cgttttttca ccacaatgac gggagttttc ctttcttcct gattttttcc cataaatacc
     9841 tcggctggct ggttttactc tttaataata tcacaatcca taaattagta aatttcccat
     9901 caggtgattt ttcatggcta ataagtccat tctctgaata tttatactcg aggtagttac
     9961 agttatggcg tgacagtcga attgatattc tactgaaccc tgatagaaac atggttgata
    10021 tattctctca ctttaatgtt gtcatgatat aaataaaaac aattcatttg aaaggatatc
    10081 agaagaagat atcggggcgc acagttcatc tctgcctttg tttcatgggg ggttctttgt
    10141 atattttact tactgtttaa gcatgtacat aaggagtgct ttcattatgc caatgatact
    10201 tgtaaagaag aatccccggg gcaaagtgat tcgggagctt agcagtgagg aagagactgc
    10261 agtaaagacg gtctgtggac tgaaaaggcc agcaaccatg gcacaacata acctggccaa
    10321 tgatcttctc cgggaaatgc gtgagtatga tgcatggtta cagtgtgact gtattccggg
    10381 agattcgccg gccatgaatt ttgctgctct taaaaacaat acgggtactc tttatctgtc
    10441 cagttttaat catgagcatg cacctgaatg ccccatgtat cgtcagttat ccggaaatga
    10501 agaggaaaca tccttcggag caagccggca tcccgttagc acccgtataa attacagaaa
    10561 ctttttacca cctgatgaca gtaattctgt tatcagactg caggcccgct ctgcttacca
    10621 taatggtgag cgctcctccg ttcgcaagaa acgtccgcgc cttggccgtt tactcctgtc
    10681 gcttattgaa gatgcagggc tgaataaact ggattccctg gctaatcccc gtatccggac
    10741 taattatcgt gagtgtcttg atgccatacg gcaggttaca ctacagcagg agtatattcg
    10801 gggaagagcc ttatctgaaa ttatccattt ccggccagga atgtctgaaa ggtctcagga
    10861 gcggttaatg gaaacgctgg agaattcaga acgacactgg cctgccagaa gaaagcatat
    10921 gttctttcag atttttatgg cgcagcacat ctgtcgtgat gctgtggaaa ttcactgggc
    10981 gaacggtaat attcaggtca tcagacctgt gcgggggatc agtattaatg gtgaagcgca
    11041 gggcggaata cgtccccctt actgggttat tcttgctttt tgccggagtg ccgatggcag
    11101 aatcatctgc agtgaagggt atgctcatgc tctttatcaa ctgacatgtc cggtgcctgt
    11161 ggacagcaaa ctggaacgaa acacgctcac tgctcttctg aatgtggcca gctggcttaa
    11221 aagaaagcca ggtacgccgg aattaagtct ggaaaggccc ctgtttgata cagaagttta
    11281 tgttaatggt gaaaagaaat atgtactgcc ggatttcatt gtcacagcaa gggctcctga
    11341 cggaaagacg gccagagtgg tcatcgaaac gatggaatat gaagacagtg attactgcgc
    11401 gagaaaatcc aggcagcata ccggcatgaa gcagattggt gttctgcata ccgatccacc
    11461 gaaatggctg gataacgatc atcccccttt taggggtctg acgctcagtg gaacgaaaac
    11521 tcacgttaag caacgttttc tgcctctgac gcctctttta atggtctcag atgtcctttg
    11581 gtcaccagtt ctgccagcgt gaaggaataa tggccgagca tattgatatg tccgtggcaa
    11641 agcggggaga ggcgtgcgat atcttcatca ttcagtgttt caccctgcgc ccggagatga
    11701 tccagggctg cctgcatata aatagtgttc cataacacga cggcgttagt gaccagcccc
    11761 agtgtgccca gttgatcttc ctgaccgtcg gtatatcgtt ttcttatctc acctttttga
    11821 ccgtgacaga tggctctggc aacggcatgg cgactttctc cccgattaag ctgggtcaga
    11881 atgcgccggc ggtaatcttc atcatcaata taattaagca gatacagcgt tttgttgatg
    11941 cgccccactt caatgattgc ctgagtcagt ccggaaggac gttcactttt cagcaatgaa
    12001 cggaccagca ctgaaacctg tactttgccc agcttcaggg agccagcggt ccggatcatt
    12061 tcgtcccact gaaggactat ttttcgggga tctgattgcc ctctggcaat atcattcagc
    12121 acgccatagt cggcatcatg gtccattcgc cagaaaaccg aagcaccggc atcagccagg
    12181 cgtggagaaa actggtatcc cagcagccag aaaaggccaa agacaagttc gctggcacct
    12241 gctgtatcgg tcataatttc ggttggattc agcccggtct cctgttccag aagaccttcc
    12301 agcacaaaga tagagtccct cagcgtcccc ggtataacga tgccatgaaa gccggaatac
    12361 tgatcggaca caaagttgta ccaggtgatc cctctgttat taccaaagta tttgcggttc
    12421 ggtccggcat tgattgttct gactggcgta acaaagcgca ttccatctgc agatgccact
    12481 tctcctccac cccatatctg tgccagtggc agcgttgcct gaaaatcaac cagtctggca
    12541 ttagcgctgg tgatagtttc agcccgcaga tagttcgctt ttgtccagtt cagccggtgt
    12601 cgggtcagtg caggaacatt tgatctgatc agtggttcca gaccgatatt gcaggcttca
    12661 gccatcagca cggcgctgat gctgacgggc agatcatcaa ctctggcact ggcttcacta
    12721 gcatggaaaa actcatcagc aaatccggta tgggcgttaa tttcgagcag caactccgtt
    12781 aaatccaccg gagggagtag atcactgatc attttgctca gtcgtttcag actgtccggc
    12841 tcatcaagac tggcgagggg agaaattgtc aaccggggct tcgggccaga aacatcgagt
    12901 tcgacagcct cattttcgca aagacgtgca gcaacctgtc tgtaacgact atcaagctga
    12961 tgacccagag attttattgc ttcctgcggg tctgtcgggt gccccaaaga acgataaacc
    13021 ttaatccggt ttgcctgcca gtcagcaccc tgtagtaatc ttgcacgagg atctccccac
    13081 cggttactgc cggtaacgta gacatccctc cgcctcagac tatcctgcag tttactgaga
    13141 aagcagagcg tgtatcccct gcgggtgata tgtttttcct tgttaatcac cagccgtttc
    13201 catgaccgac tgataatttc cgttggtgcg tcgtcaaaaa actgccgccg tgagctgaac
    13261 tcccggctga ggtagtcaca ggcattcaga gtggtaaccc cggcaggtgc ggatgaaaat
    13321 ttaacggtat tcagcagatg gggcaggaaa cgacgaacgc gcccgtactg ctccaccatt
    13381 tcttcatgaa aattatcgtc tgagggccgg gcaatttcac ggacaagcgt gatgatttca
    13441 gccagctttt gccttgggat gtagctgaac acctcagcac gaatcgattc gtccggtgtt
    13501 tcttctttca gcaggtacga acatgcgctg gcgagcgcca atgcagattt atccagatcc
    13561 ttcagcgagc ggagccgttt tttctgccca atctttctgg cgtcacggat gataacggcc
    13621 agcatggcgt ccagaacgtc caatgcatca tccagcgcca gcgtttccca tgcaaggaca
    13681 aaggcaacca gaaccgccat ccttttctgc ggtgacatcc tggcaatatt gaacaccgaa
    13741 gtcataccag cataacgtgc gagatttttc aggcgcacag ccgggagtgt actcaggttt
    13801 tcagcatgca ggccaaaatc gttcagagtt ttccagcgtt caattgcttc attaaacgcc
    13861 ggaccactga tggtcacagg gccctttttc agtgattcca gtaaagacag gcggctgcaa
    13921 tcagttggcc ccagcagcat ctccagctgt gaacgctgtt cggctgacgg tatcagtgcc
    13981 agtttgttcc acaggcgcaa cgtcgccttt tcccttacct ctgaaatcaa ccgggtcagc
    14041 gtagtggctc cggggagaat aatacgatgt tgcataagcc accctgtcgc cagatcgaaa
    14101 agcaggccag gacgttcgtt gcttatccag ctccgggtat ataaaagacg ggtaaggcga
    14161 aatgtccagg gccaggcaaa ttcacgatac tgatagtgct gacgtatcag cgctgcatgc
    14221 tcacggcggg tattttccct ctgaccgtat tctgcaagaa cggtgatatc acgaatcccg
    14281 agctgtctgg cggtaaaatg ccggacgccg gaaggaatat gattcatatc ggtgaggaag
    14341 gtgcccagaa aacggacaca tccaatttgc agggcaatgc ccagacggtt gtgatcacct
    14401 ctgctttttc cgataaattc cttgtctgct tcatcaaggt gaaaatatcg tgccagctga
    14461 agctcatccg gttcaccggt gaatctgcca tagctttcag tctgctcagt ggtcagaaag
    14521 tcaacgggca tatcggcctc cctgcctgac gggcatttag taacattttt ccaaccgtac
    14581 gaaatgttat aaattatcgg acatcgtaaa actgttacat taatatgtct attaaatcgt
    14641 aaatttgtaa taatagacat gagttgtccg atattcgatt taaggtacat ttttatgcga
    14701 ctttttggtt acgctcgggt ctcaaccagt cagcagtctc ttgatcttca ggtcagagca
    14761 ctcaaagacg caggtgtgaa agcaaaccgt atatttaccg ataaggcatc cggcagttca
    14821 acagaccggg aagggctgga tttgctgagg atgaaggtgg aggaaggtga tgtcattctg
    14881 gttaagaagc tcgaccgtct tggccgcgac actgccgata tgatccaact gataaaggaa
    14941 tttgacgctc agggcgtggc agtccggttc attgatgacg ggatcagtac cgacggtgat
    15001 atggggcaaa tggtggtcac catcctgtcg gctgtggcac aggctgaacg ccggaggatc
    15061 ctagaacgca cgaatgaggg ccgacaggaa gcaaagctga aaggaatcaa atttggccgc
    15121 aggcgtaccg tggacaggaa cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg
    15181 gaaattgctc atcagctcag tattgcccgc tccacggttt ataaaattct tgaagacgaa
    15241 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    15301 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    15361 tacattcaaa tatgtatccg ctcatgagac aataaccctg gtaaatgctt caataatatt
    15421 gaaaaaggaa gagtatgagt attcaacatt ttcgtgtcgc ccttattccc ttttttgcgg
    15481 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    15541 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    15601 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    15661 gtgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
    15721 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    15781 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgct gccaacttac
    15841 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    15901 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    15961 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
    16021 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    16081 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    16141 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    16201 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    16261 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    16321 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    16381 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    16441 ccttttaaga aacatatgta cggtgttttt atgcatctca ggtactgaga tattttgtgg
    16501 ctcagttctg taacttttcc cgtaacattg tctgttgtta cgggaaagtc cggtttttgt
    16561 attgcaccag agaataccca gactgtgatg ctgccacagc gtcagcaggc tttctgaacg
    16621 gtgtaacatc acttcatgtt aatgataatc actatcatca aatcttgaca tgccattttc
    16681 tccttaataa attaatactg tatatgtatc catatgcgta agcagttaat tcatttgttt
    16741 tcctcagagg atgaaggaga taccgaatgt ctgaccctgt acgtattaca aatcccggtg
    16801 cagaatcgct ggggtatgat tcagatggcc atgaaattat ggccgttgat atttatgtaa
    16861 accctccacg tgtcgatgtc tttcatggta ccccgcctgc atggagttcc ttcgggaaca
    16921 aaaccatctg gggcggaaac gagtgggttg atgattcccc aacccgaagt gatatcgaaa
    16981 aaagggacaa ggaaatcaca gcgtacaaaa acacgctcag cgcgcagcag aaagagaatg
    17041 agaataagcg tactgaagcc ggaaaacgcc tctctgcggc gattgctgca agggaaaaag
    17101 atgaaaacac actgaaaaca ctccgtgccg gaaacgcaga tgccgctgat attacacgac
    17161 aggagttcag actcctgcag gcagagctga gagaatacgg attccgtact gaaatcgccg
    17221 gatatgacgc cctccggctg catacagaga gccggatgct gtttgctgat gctgattctc
    17281 ttcgtatatc tccccgggag gccaggtcgt taatcgaaca ggctgaaaaa cggcagaagg
    17341 atgcgcagaa cgcagacaag aaggccgctg atatgcttgc tgaatacgag cgcagaaaag
    17401 gtattctgga cacccggttg tcagagctgg aaaaaaatgg cggggcagcc cttgccgttc
    17461 ttgatgcaca acaggcccgt ctgctcgggc agcagacacg gaatgacagg gccatttcag
    17521 aggcccggaa taaactcagt tcagtgacgg aatcgcttaa cacggcccgt aatgcattaa
    17581 ccagagctga acaacagctg acgcaacaga aaaacacgcc tgacggcaaa acgatagttt
    17641 cccctgaaaa attcccgggg cgttcatcaa caaatcattc tattgttgtg agcggtgatc
    17701 cgagatttgc cggtacgata aaaatcacaa ccagcgcagt catcgataac cgtgcaaacc
    17761 tgaattatct tctgagccat tccggtctgg actataaacg caatattctg aatgaccaga
    17821 atccggtggt gacagaggat gtggaaggtg acaagaaaat ttataatgct gaagttgctg
    17881 aatgggataa gttacggcaa agattgcttg atgccagaaa taaaatcacc tctgctgaat
    17941 ctgcggtaaa ttcggcgaga aataacctca gtgccagaac aaatgagcaa aagcatgcaa
    18001 atgacgctct taatgccctg ttgaaggaaa aagagaatat ccgtaaccag ctttccggca
    18061 tcaatcagaa gatagcggaa gagaaaagaa aacaggatga actgaaggca acgaaagacg
    18121 caattaattt cacaacagag ttcctgaaat cagtttcaga aaaatatggt gcaaaagctg
    18181 agcagttagc cagagagatg gccgggcagg ctaaagggaa gaaaatacgt aatgttgaag
    18241 aggcattaaa aacgtatgaa aagtaccggg ctgacattaa caaaaaaatt aatgcaaaag
    18301 atcgtgcagc gattgccgca gcccttgagt ctgtgaagct gtctgatata tcgtctaatc
    18361 tgaacagatt cagtcgggga ctgggatatg caggaaaatt tacaagtctt gctgactgga
    18421 tcactgagtt tggtaaggct gtccggacag agaactggcg tcctcttttt gttaaaacag
    18481 aaaccatcat agcaggcaat gccgcaacgg ctcttgtggc actggtcttc agtattctta
    18541 ccggaagcgc tttaggcatt atcgggtatg gtttactgat ggctgtcacc ggtgcgctga
    18601 ttgatgaatc gcttgtggaa aaagcgaata agttctgggg tatttaaata aaatataaga
    18661 caggctgtct atcttacaga cagccttttt ataataacag aaagaatata tatcatactt
    18721 aacggaagag ataatatgaa aacagtaccc gtataaaccg caagcaaccc catttttcca
    18781 ggtgtatcag caaaaaaacc gctgttccag aaatcaggcc gggtaaattt cagagctgta
    18841 tcttcaatat accattttgc aaccggatac aaaaccattc cgcagagaga aatacaccag
    18901 aacagtaatc tgtatttaaa ctcataatcc catgacatat acagcatata tccccccgtc
    18961 acccatcccc accacatatt attaaaataa tattttctgt tcatctgcca gttttccttt
    19021 cttttgcact tttgtcagtg tactgatgca tgacaacata accacagcat gataataata
    19081 atcaataaca ataagctgtg tcacgtttac atcatggaac ggaggtgctc ctgtgacctt
    19141 tgagcagaaa aaagcccggg ccattgccct gatggacagc aaaaagatgt ggcggagcaa
    19201 ttatgcccct cctcttctgc gtattctatg gcggctgggt atccggcttc cgccattacc
    19261 ctttatgccg ttctggcagg tcacggtgct gacggggggc ttatggggca tttcctgggg
    19321 atgtgtaatg tggtttacct actgggggcc gtcaggaatg gtggcaggtg aggccattat
    19381 tataagtatc acgggtggtt ttttgttcgg actgtgcatg gcctctttcc actggtggcg
    19441 caggaaagtt aaccggctgc caccatggga tgatgtgtaa accgtgcaca ggcgtgttct
    19501 tacggagcgg cactttcatt tatctgtacg cgaacctcta ttcctgcgtc atgacaggcc
    19561 tgcagccact gcgccacttc cagcggatcg ccctcccggc gtaccaccct gccttcttta
    19621 ttccataact gcagacaggt gctgccgtcg agacgcacca caaaatcccc gcggcaggcc
    19681 tgatacgtta tgccgttcag ctccaccgcc agatcagtgg cagaagtgcc gtctgcgggc
    19741 agacctgctg ccgggaccag cggcagtacg acgacttctg ccgcacgctg ttctgcccgg
    19801 aggcggtcct gttctgccag cagcagaggc tgtgccacgg cccggccggc gtccgtcagc
    19861 tccactgcca gctgcaggtt cggggcgcgc agggtacgca gccagccggc atcctccaga
    19921 cgacggcagg aggcgcgcag gtttggcccg tagaccgggg cttccccgct tcgctccagc
    19981 acccgttcca ggtcccgcgt cagcacgggg cccggccgtt tttcatcaag cgcagccagc
    20041 acaatcagca ctctccgctg cagtggcgac ggtcggcggc tctgagtcat taactgtcac
    20101 cctccgacca gatgagtttt ttgaggcgct gataatcggt atcgatgcgt tcccgggcct
    20161 gtgcgtgatt gttctgtggc gcaccatgaa tggccagttc ataccacaac cacagcagag
    20221 ctgaggcctt cagttcactg atacacctgg cgctctggtt gtccagccca cattcacgga
    20281 tatttttttc cgctattcgg gttagttgct catagctgag tgaaaacacc ctttcttcat
    20341 ccatcctgca cgctccatct gataatgttc tgacaggtaa aaataatatc ctgtatgatt
    20401 tgtgtgctcc tgatttctga taatatcaga tatggtttta tcatcaatga tgaaaatcat
    20461 atctgatatg attttgtgtg tattgctcaa ttaccatatc acttgcccgt ttcccgccgc
    20521 gcagatccgg tttccggcgg ttcaggatct tcctctcccc ccacataacg ccacctcctg
    20581 tcagcacaac atgtggtatc tcattcagct gcttatgaca ctatatgttg tgtcatctcc
    20641 ctgacctgtg atgcgccgcg caggggcgga aaacagcgat atgattattt tctcagcgtg
    20701 gtacacttcc ggaaagttgt gatattccgg aaagtcggat ctgacggaaa cggctctccg
    20761 gtaatttaac ggcgtggtta tatggatgct tgttatcatg gtgatgatga taacggcatg
    20821 atgttatcag acggcgtgac ggtaagggca gtgatgatgg atgacgttat cgcatgaccg
    20881 tccctgcccg gaaaagaaaa aaggagtcac ccatgttttt tattgagaat gaaggtcagg
    20941 ctgttgccag aacggattac tggcagtctg tacaggcgca ggccggatat gtctacctca
    21001 gctggaatgc cggcgcagcc aggctgcttg tcccggatgc ggcaaaacat ttactcaggg
    21061 agatgcgggg ggctgagtac gtcatcatca gtaagggaac gctgcatggc cgcgatgcgc
    21121 tggaactggt atttgaagac ggcagcgatg cgccgttcgt gatccacatg ctgagtgagc
    21181 agtgcgatcg cctgctcccc gaaaacaacc agggaggggg gtttgttgtc accgtctgga
    21241 cgcgtggcgg taaccagctc cgttatccgg gaaagtaccg ggttgtggaa aacctgcccg
    21301 acgtttcccc gtggagtgaa cactgatatg cagcacctgc cagcaccgat ccaccatgcc
    21361 cgggatgccg ctcagcttcc tgttgccatc gattatccgg cagcgctggc actccgccag
    21421 atgtcgatgg ttcatgatga actgcccaaa tacctgctgg cccctgaagt gagcgccctg
    21481 ctccattacg tcccggatct gcgccgcaag atgctgctgg ccacactgtg gaacaccggt
    21541 gcgcgcatta atgaagcact ggcgctgacg cggggggatt tttcgctcac gcccccgtat
    21601 ccgtttgtgc agctggccac tctgaagcag cggacagaaa aagccgccag gacggcagga
    21661 aggatgcccg ccggtcagca gactcaccgg ctggttccgc tttccgacac ctggtacgtc
    21721 agccagctgc agacgatggt ggccacactg aaaatcccca tggaacggcg taataaacga
    21781 acaggcagga cagagaaagc gcggatctgg gaagtgacgg acagaacggt caggacctgg
    21841 attggggagg cggttgccgc cgctgccgct gacggtgtga cgttctctgt cccggtcacg
    21901 ccacatacgt tccgccattc ctatgcgatg catatgctgt atgccggtat accgctgaag
    21961 gtcctgcaga gcctgatggg gcataagtcc atcagctcaa cggaggtcta cacgaaggtg
    22021 tttgcactgg atgtggctgc acggcaccgg gtgcagtttt cgatgcctga gtccgatgcg
    22081 gtgtctatgc tcaaaagaat tccataagct ctgacttttt taaatccctt tcggatatct
    22141 catggatata ttatcggtat ttcatattct gttgacatgc aggaccggta tagagtaaaa
    22201 aaacatctat tgagtacctc ttggatatcc gaaaggtgtc ttatggggtc tttatgggta
    22261 ttacatgtgg attctggaga tacactatgc ctgttattgc taatacgcat cccaaaggtg
    22321 gtgtgggtaa gacaacttca tccgtaaaca ttgttggtga aatgaagtct gatacagttg
    22381 atctggatac tcataccggg ctttctatca ttctggggct gagacctgaa ggaaaagaaa
    22441 tttccgtgaa agtacccaaa acagtggatg agttaatcga aattatgact ccctacaaga
    22501 acagcgataa gacgttactt attgactgtg ggggatttga ctcagatctt acccgtactg
    22561 cgattgcctt tgcagactgc gttattgtcc cttcaaaaga ctccctgact gaacgtattg
    22621 gtttgatgca ttttgatgga gtactggacg aaatcagctc tatcatgggg accgatataa
    22681 ctgctcacct gtatctctgt aaagttaatc ctaacaagaa gaaattccct aagcttgatg
    22741 caatcctgcc atcgttcaaa catctgaagc tgatgaagag ccgcatttct gctcgtgcag
    22801 aatttgagga tgtcattgag actgggatgg gaatcactga gtctgttcat gggcgctatt
    22861 cggcaggagg taaggaagtt attgccctga tcgaagaaat caatcatctg attgaaaata
    22921 acaaacagta aggtgtgtct attgggtatc catatagtat cctttgggct tttaatggat
    22981 atcttttaag tatctaacaa acatcctggg ttatctcatg agcaaagcga aatttagtgc
    23041 acaggctgaa aaactgaaaa acctggtgga ttctgccaga ttacaaactt cttcttctgc
    23101 cgtaacagca acagccccgt ctcagccaaa tgaaggtgca gaggaagttt caggtcgtcc
    23161 cgtggctgcc aggacgaagg ctaagaatct taaggccata ccgctgtcgt attttgaagc
    23221 tcatgcggaa cttaaagcga ccagtaaaac atctctggat tttagctcat atattattga
    23281 ggctataaga gagaagcttg aacgtgatgg tgcaataggt caataaacta tcggatacct
    23341 taagggtatc tgatgtgtgt cttttaaatg cgttatgggg attcttcacg tacctaaaga
    23401 gtccccatta gacatctttc tgtttatggt gacatgattc gtcaccgctt tcctttcctc
    23461 tcagtttcac catttcattc cttttccctt aacctgaacc atgatgtcca cgatgttttt
    23521 atattgcatt tatgtgtcac atataacctt cataatgttt ttgtatctat gatttcctct
    23581 ggtcttaaag ttgtgtgtac aatgtacatg caatttatcg tgtttttatg ttgcatttgt
    23641 cctttcagaa agaccgtttt ttcattataa ttgaatatac attgcatgta tgaggcatgt
    23701 ttgtttttct atttgtttat gagcaacgga gactggttat gaaaatcttt attgatgatg
    23761 gttctaccaa cattaaactt gcctggttgg aggatggtga cgtgaaaacg ttaatcagcc
    23821 ctaacagctt taaaccggaa tggtctttta gtttactgga tgatgctgct ccggctaatt
    23881 acgagatcga cggcgagaag ttttcatttg atccattaag tgctgatgct gtcgtaacga
    23941 ctgaaacacg ctatcagtac agtgatgtga atgttgtggc cattcagcat gcgctacagc
    24001 agaccggcct gaaagcgcag cctgttgatg tcatcgtcac attgccgatc tctgaatacc
    24061 ttgacgccaa caatcagaaa aataagcaaa acattgaaag aaagaaaaaa aatgtgatgc
    24121 gtgaagtacg ggtgcagggg agtgatgcat ttgttattcg cagtgtttcc gttcttcctg
    24181 aaagtattcc cgctggtttc agtgttcttg cggggcttga ggatgatgaa tcccttttga
    24241 tcgtagatct cggtggtaca actcttgatg tgtcgcatgt tcgtagcaaa atgacaggga
    24301 ttacaaaaac ctggtgtgat ccgaatatcg gcgtgtccct gattacatcc ggggttaagg
    24361 aacagatggc tgtacatgct aatacaagag tgagctcatt ccaggctgac aatatcattg
    24421 tgcaccgtaa tgaacctgac tacctttccc gtcgcattta taatgctgaa cagcgtgagt
    24481 ctatcattaa tgttatcaat gaacggcaga agttgcttat taaacgagtt aatgatgtca
    24541 tcagccgttt caccgactat acacatgtaa tgtgtgttgg tggtggggct gaaattgtgg
    24601 ccgaggctgt gaagaacctg actaaggttc ctgatgagcg cttttatctg agctcatccc
    24661 cgcagtttga tttagtcatg ggaatgataa aaatgaaagg tggtgtgact aatgagtgat
    24721 gaaaataaat ccagacgctg ctcatttgaa cttttcccgg atgaacggac aggagataag
    24781 atagcagatg agctcattgc aaatgaaaaa ctgaaggagc ggggacgttt catgcgcgcg
    24841 atgttagtta ctggtgcggc gtttgcagct atagataaac gtcttccttt gctgatatcg
    24901 gagttactga cagaaaatac gactctggat gatattaata aggtgatttc cagcgtaata
    24961 ccaggtgctt tttcggttga aaagaaattg cttgaattac tggaaaaaca atctggtctc
    25021 catacgtctg tggattgcag tactcctctt acagagcaga gtctgtcgag aaatgacggt
    25081 gaagaccaga ccagaagaaa cgctgaaaat atgttcggag atgactgaac taacccagcc
    25141 tggccagggg aatatctcgc catttagttg tataacactg actcaacatt tcccgtttca
    25201 tttgccattc aggggcggtt cctcgccccg caaaccacac tttccctttt cccgactggt
    25261 tcagttcatc gagtgttttc attaactttt cactgttttt acggggctgg atttcatcaa
    25321 ataatcccgg ctgcgctata cccgatggtg tgaaatcagc cagcatgact cctgccttca
    25381 tatagcggta cccttcacgc cagacatggt ttaaggctct gcatgcggcg gcaataatgt
    25441 cccggctgtc ctgtgtgggc aatggaagct tttccacagc ggcattgctg taacagggtt
    25501 cttttactgc aaagggggat gtccgtacaa atgtcgtcac ctgccggcaa tactgacgct
    25561 ccccacgtag tttctctgcg gcccgctctg catactgaac aacagcctgg tgcatggcat
    25621 ctttgtctgt gattcgttca ccaaaactgc gactacagac aatctgctgt tttgccggtg
    25681 gtgcttcttc cagggatatg caggactcgc cgttgagttc gcgtaccgta cgctcaagaa
    25741 tgacgctgaa gtttttccgg atgaatgccg tgttagcctg cgccagctgc agtgctgtgt
    25801 taatacccag cgcattcagc ttttccgtca gtctgcgtcc tactccccag acctcaccaa
    25861 ctggctgcag ccccagtagc ttcaaggtcc gattacggtt ttctgccgtc agcgcgacca
    25921 ctccggaaaa ctgtggccat tgctttgttg cccactgtgc acttttagcc agcgtttttg
    25981 taggcgcaat gcccaccccc atggtgagtc ctgtccagct ctttacctgt tccctgagct
    26041 gatgaccaaa aacctccgga gagatgcaat gatttatccc ccgcaaatca atgaacattt
    26101 catcaattga gtagggctca actgcgggag aaagcgactc cagaacagcc atgacccgtt
    26161 ggctcatgct gtggtacagc gcataattgc tggaaaatac atgtattttc ttctccaggc
    26221 gcatctgtct cacctgaaac cagggctgcc ccattctgat gccaagggct tttgcctccg
    26281 ggctgcgcgc gatcacacag ccatcgttat tgctgagtac gatgaccggt tcgttgcgaa
    26341 ggtccgggcg gaaaactttt tcacatgagg cgtagaaact gttgatatca gccagtgcaa
    26401 acatcagcgt aactcccgcg tcctgtgtat cacgtgagtg acaacaccaa aaatacagat
    26461 gttttccgga tacagtgtgc ggaattccgg gctgtctgaa accggctcca gtgccgggcg
    26521 tgggcgcaac agcagtcgtt tgacggtgaa ctcaccgtcg atctcagcga taacgatgtc
    26581 cccgtgttgt ggtttctcgg ccctgtccac taccagcaga tcaccattct gcacgccagc
    26641 ctggttcatc gattcaccgc tggcgcgcag aaagaaggtg gctgcaggtc tgctgatgca
    26701 atagctgttc agatccagtt cctgctcagc ataatcagtg gcaggcgaag gaaaaccggc
    26761 ctggcaacga tcggcaaaca acggtcgcac gtaactgtca tcgcctgaag ggtcagccgg
    26821 acggtgataa acggtactca tgctgcaggc agccagctgt cgtcctgcca gatttcttcc
    26881 agcagagaca ggatacgttc tttatccttg tcgttgcggg ctcctgtgac agatacagac
    26941 ggggcgctgc cctggtctat acgcagccag atttccgggt agtgtggttt cagacgttgt
    27001 tcgatttcat tctgtagcgc ctgaagtgtg cctgatttga ggttttttgt gctctggcgg
    27061 tcgaaaagaa tttcaatgcg gatcatgttc tcatctccct tttctattgc tgtatgcata
    27121 tacagtattc taataactga atccacacac agtcaaatgt cattattgtg actaaaaaac
    27181 gctcaattct ctgggagtgt ggctgtatgc ctgctgtgga ctgctgtgtc cagcttatcc
    27241 acagcatttt gcgcacggtt ctgtgaataa aatacctggt tacccaggcc gtgccggcac
    27301 gttaaccggg ctgcatccga tgcaagtgtg tcgctgtcgc cggcctcctc acccggtcac
    27361 gtttcgtcgt tcctcctcca cgcgctgcgg cttcggggcc gcacctgcat tcgtatgcgg
    27421 tcgcccggtt acaggtgcgg cacggcctga tggaggccgc atgtgagagg agaattccca
    27481 tgccaaactg gtgctcgaat cg
//
LOCUS       DAZFTN010000063        27164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFTN010000063 DAZFTN010000000
VERSION     DAZFTN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..503
                     /gene="ybeT"
                     /locus_tag="ACHKV2_002942"
     CDS             <1..503
                     /gene="ybeT"
                     /locus_tag="ACHKV2_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6940796.1"
                     /translation="CISQPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIHGH
                     STSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDT
                     RKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLL
                     AGLPAH"
     gene            complement(513..1940)
                     /gene="djlB"
                     /locus_tag="ACHKV2_002943"
     CDS             complement(513..1940)
                     /gene="djlB"
                     /locus_tag="ACHKV2_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO6940797.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1937..2644)
                     /gene="ybeR"
                     /locus_tag="ACHKV2_002944"
     CDS             complement(1937..2644)
                     /gene="ybeR"
                     /locus_tag="ACHKV2_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6940798.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2809..3786
                     /gene="ybeQ"
                     /locus_tag="ACHKV2_002945"
                     /pseudo
     CDS             2809..3786
                     /gene="ybeQ"
                     /locus_tag="ACHKV2_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3856..4338)
                     /gene="ybeL"
                     /locus_tag="ACHKV2_002946"
     CDS             complement(3856..4338)
                     /gene="ybeL"
                     /locus_tag="ACHKV2_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO6940799.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4574..7156
                     /gene="leuS"
                     /locus_tag="ACHKV2_002947"
     CDS             4574..7156
                     /gene="leuS"
                     /locus_tag="ACHKV2_002947"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO6940800.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7171..7752
                     /gene="lptE"
                     /locus_tag="ACHKV2_002948"
     CDS             7171..7752
                     /gene="lptE"
                     /locus_tag="ACHKV2_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO6940801.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7752..8783
                     /gene="holA"
                     /locus_tag="ACHKV2_002949"
     CDS             7752..8783
                     /gene="holA"
                     /locus_tag="ACHKV2_002949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO6940802.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8785..9426
                     /gene="nadD"
                     /locus_tag="ACHKV2_002950"
     CDS             8785..9426
                     /gene="nadD"
                     /locus_tag="ACHKV2_002950"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO6940803.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            9450..10061
                     /gene="cobC"
                     /locus_tag="ACHKV2_002951"
     CDS             9450..10061
                     /gene="cobC"
                     /locus_tag="ACHKV2_002951"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO6940804.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10321..10638
                     /gene="rsfS"
                     /locus_tag="ACHKV2_002952"
     CDS             10321..10638
                     /gene="rsfS"
                     /locus_tag="ACHKV2_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO6940805.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10642..11109
                     /gene="rlmH"
                     /locus_tag="ACHKV2_002953"
     CDS             10642..11109
                     /gene="rlmH"
                     /locus_tag="ACHKV2_002953"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO6940806.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11140..13041
                     /gene="mrdA"
                     /locus_tag="ACHKV2_002954"
     CDS             11140..13041
                     /gene="mrdA"
                     /locus_tag="ACHKV2_002954"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO6940807.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13044..14156
                     /gene="mrdB"
                     /locus_tag="ACHKV2_002955"
                     /gene_synonym="rodA"
     CDS             13044..14156
                     /gene="mrdB"
                     /locus_tag="ACHKV2_002955"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO6940808.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14167..15255
                     /gene="rlpA"
                     /locus_tag="ACHKV2_002956"
     CDS             14167..15255
                     /gene="rlpA"
                     /locus_tag="ACHKV2_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO6940809.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15395..16606
                     /gene="dacA"
                     /locus_tag="ACHKV2_002957"
     CDS             15395..16606
                     /gene="dacA"
                     /locus_tag="ACHKV2_002957"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO6940810.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16716..16979
                     /gene="ybeD"
                     /locus_tag="ACHKV2_002958"
     CDS             16716..16979
                     /gene="ybeD"
                     /locus_tag="ACHKV2_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO6940811.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17080..17721
                     /gene="lipB"
                     /locus_tag="ACHKV2_002959"
     CDS             17080..17721
                     /gene="lipB"
                     /locus_tag="ACHKV2_002959"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO6940812.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17981..18934
                     /gene="ybeF"
                     /locus_tag="ACHKV2_002960"
     CDS             17981..18934
                     /gene="ybeF"
                     /locus_tag="ACHKV2_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO6940813.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19143..20108
                     /gene="lipA"
                     /locus_tag="ACHKV2_002961"
     CDS             19143..20108
                     /gene="lipA"
                     /locus_tag="ACHKV2_002961"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO6940814.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20209..20412)
                     /gene="tatE"
                     /locus_tag="ACHKV2_002962"
     CDS             complement(20209..20412)
                     /gene="tatE"
                     /locus_tag="ACHKV2_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO6940815.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20541..21329)
                     /locus_tag="ACHKV2_002963"
     CDS             complement(20541..21329)
                     /locus_tag="ACHKV2_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO6940816.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21422..21805
                     /gene="crcB"
                     /locus_tag="ACHKV2_002964"
     CDS             21422..21805
                     /gene="crcB"
                     /locus_tag="ACHKV2_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO6940817.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21859..22068)
                     /gene="cspE"
                     /locus_tag="ACHKV2_002965"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21859..22068)
                     /gene="cspE"
                     /locus_tag="ACHKV2_002965"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6940818.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22243..22803)
                     /gene="pagP"
                     /locus_tag="ACHKV2_002966"
     CDS             complement(22243..22803)
                     /gene="pagP"
                     /locus_tag="ACHKV2_002966"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO6940819.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23392..24777
                     /gene="dcuC"
                     /locus_tag="ACHKV2_002967"
     CDS             23392..24777
                     /gene="dcuC"
                     /locus_tag="ACHKV2_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6940820.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24818..25498)
                     /gene="dpiA"
                     /locus_tag="ACHKV2_002968"
                     /gene_synonym="citB"
     CDS             complement(24818..25498)
                     /gene="dpiA"
                     /locus_tag="ACHKV2_002968"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO6940821.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25467..27125)
                     /gene="dpiB"
                     /locus_tag="ACHKV2_002969"
     CDS             complement(25467..27125)
                     /gene="dpiB"
                     /locus_tag="ACHKV2_002969"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO6940822.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6891 a         6824 c         6641 g         6808 t
ORIGIN      
        1 cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct
       61 ttgattattt aagctcaccg ggcagtcttc cattcaccac ggcagccacg gagctttccg
      121 cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt catggtagcg
      181 acggtaaacc actggattat acacaggcga gatactggta tgagcaatca gcggaacagg
      241 aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg ggggtcaaac
      301 ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg
      361 ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt aaccattatg
      421 aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac
      481 ttgccgggct tcccgcacat taacgtcgac tgtcacccga acagatggag tattttccct
      541 atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag acagcctggc
      601 ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt ttgcttctcg
      661 gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga
      721 ttttctgcat caagacggct tacgatcggc attaatgatt cttctgtagg ttggctggcc
      781 tgcactaatt gtttggcaac tagatataat ggccataagg tatgttctgt atcacgcgtg
      841 agtgagatcc gcggcttaca tcctttgtgc aaaaccatct cgaccagtga acgccccaga
      901 gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg
      961 ccggggacat aaagtagcgg ttttccctgg tcgttaacca gttcacgagc ttcaatcatc
     1021 atcaccacta atgggtaata atcgactcga tgctgacggc accattgcaa cattaaagca
     1081 tccgcctgag tggagggata ctcgtgccag tagtcacaaa gttctgccag caggctttcg
     1141 ccttctccac aatagacgcg ttgcgcgtat tcataataac gcgcatcttc attatcaggt
     1201 tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcgacctgca
     1261 ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga
     1321 tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg ggcgaacatt
     1381 tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat gatctggaag
     1441 ttaaaaacat ttcccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt
     1501 tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg
     1561 aaggataaat gggcagtgtt catggctatt gtgcacagcg accagcgtaa ttcatcaatc
     1621 tcattcatcg agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa
     1681 cgttcactat cagaggcaag taacgcacga aaggcgagca gaatttcatg ttctgctacc
     1741 tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg gagcgcttcc
     1801 tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt
     1861 aacagcgcca gataagcgcg gcggataata tcgacatcag tcgtttcctc tatgttgagg
     1921 atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg catgtggggt
     1981 agtcagccag tggttgccag ggtaaggtcg cgtaatatgc atcgtcactg gcaaataatt
     2041 gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat tcacgtaata
     2101 aaggcagatt atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa
     2161 agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc
     2221 atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca
     2281 tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga
     2341 caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac
     2401 atggagaata acgaaaccag cggtgataaa accctgccaa ccgcgcggca tgaccgttat
     2461 cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc caggaggaag
     2521 aaaggtcaaa aaagcgtgta cctaaataca ttttgggtga tgaatgcgaa ggtaaacagc
     2581 attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gactccatgt
     2641 ccatatgatt tatccttaaa tattcattga atgaatatta gccgcattgc gacgctcgtt
     2701 tatttatact tttctgacgc gacataatac gcataaaata aggcttgcgc aaaaaatgaa
     2761 acacataaaa attcaatata ttttatctac aatcataagg aaatgataat gattttcacg
     2821 tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga aaaaggcgat
     2881 tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat tgactctccg
     2941 gacgacgaaa aggcctttta ctagctgaag ctggccgctg agcaaggtca ttgtgaagca
     3001 cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga taatgagcaa
     3061 gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc caacgccctt
     3121 ggctggatac tggatcgtgg agaagacccc aactataaag aagcggttgc ctggtatcag
     3181 atagccgcgg agagcggaat gtcttatgcg caaaataatc ttgggtggat gtacagaaat
     3241 ggcaacggag ccgcacaaga ctatacgctg gcatttttct ggtacaaaca agctgcatta
     3301 caaggccata gtgacgcgca aaacaatctg gccgatcttt atgaagacgg aaaaggcgtt
     3361 gctcaaaacg agacactcgc cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg
     3421 catgctcagt ttcaaattgc atgggattat aacgctggcg aaggggtgga ccaggactat
     3481 aagcaagcga tgtactggta tctgaaggct gccgctcagg ggagcgtcgg cgcttacgtc
     3541 aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca ggctgccttt
     3601 gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa cctggccatt
     3661 atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga cctgtatcgt
     3721 aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac tgaagattta
     3781 ctgtagagat aaaaacaagg gccggggaat ttcattcggc ccatattctc tttattgctg
     3841 tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat gaccacattt
     3901 cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac atttctcgca
     3961 gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat gattgaggtc
     4021 ctggaaaact tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca
     4081 caagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca ggctctcttc
     4141 atagctcatg gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgacctc
     4201 ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc
     4261 gatatcacgt tcgccattgc gcaggcgttc gctcagtgac gcaaccagtt cacggtaata
     4321 ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg
     4381 ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg tatgaataat
     4441 ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac gtttgggcta
     4501 tgctatgcgg atctgaaaaa ccacctctaa cgctactttt gtagccgtaa tgaaaacagg
     4561 accactggct gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct
     4621 tcattgggat gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta
     4681 ctgcctgtct atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta
     4741 caccatcggt gacgtgatcg cccgttacca gcgcatgctg ggcaaaaacg ttctgcagcc
     4801 gatcggctgg gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc
     4861 tccggcaccg tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg
     4921 ctttggttat gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga
     4981 acagaaattc ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcggt
     5041 caactggtgc ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg
     5101 ctggcgctgc gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac
     5161 tgcttacgct gacgagctgc tcaacgattt ggataaactg gatcactggc cagacaccgt
     5221 taaaaccatg cagcgtaact ggatcggtcg ttccgaaggc gtggaaatca ccttcaacgt
     5281 taacgactat gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg
     5341 tacctacctg gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc
     5401 tgaactggcg gcctttattg acgaatgccg taacaccaaa gttgctgaag ctgaaatggc
     5461 gacgatggag aaaaaaggcg tcaatactgg ctttaaagct gttcacccat taacgggcga
     5521 agaaattccc gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagtaat
     5581 ggcggttccg gggcacgacc aacgcgacta cgagtttgcc tctaaatacg gcctgaacat
     5641 caaaccggtt atcctggcgg ctgacggctc tgagccagat ctttctcagc aagccctgac
     5701 tgaaaaaggc gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt
     5761 caacgccatc gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg
     5821 cctgcgtgac tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac
     5881 gctggaagac ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga
     5941 agatgtggtg atggacggca ttaccagccc gattaaagca gatccacagt gggcgaaaac
     6001 taccgttaac ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc
     6061 ctcctggtac tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga
     6121 agcggctaac tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat
     6181 gcacctgctc tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc
     6241 tgacgaacca gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta
     6301 tgttggcgaa aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga
     6361 cgagaaaggc cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg
     6421 catgagcaaa atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg
     6481 ttacggcgcg gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct
     6541 cgaatggcag gaatccggcg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact
     6601 ggtgtacgag catactgcga aaggtgatgt tgcggcactg aacgttgatg cactgactga
     6661 agatcagaaa gcgctgcgtc gcgatgtgca taaaactatc gctaaagtga ccgatgatat
     6721 cggccgtcgt cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact
     6781 ggcgaaagca ccgaccgatg gcgagcagga tcgcgctctg atgcaggaag cactgctggc
     6841 cgttgtccgt atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact
     6901 gaaaggcgaa ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt
     6961 ggaagactcc acgctggtcg tggtgcaggt taacggtaaa gtccgtgcta aaatcaccgt
     7021 tccggtggac gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc
     7081 aaaatatctt gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa
     7141 tctggtcgtt ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct
     7201 ctggcggtgt taatcaccgc cgggtgtggc tggcatctgc gtgacaccac gcaggttcct
     7261 tccactatga aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg
     7321 gtgcgtaacc agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag
     7381 gacgttccat ccctgcgttt gggtgctgta tcaatctcac aggataccgc gtcggttttc
     7441 cgtaatggtc aaactgcgga atatcagatg gtgatgactg ttagcgcgtc ggtattgatt
     7501 cctggacgag atatctaccc gattagcacc aaagtcttcc gttcattctt cgataacccg
     7561 caaatggcgt tagcgaaaga taacgagcag gaaatgatca ttaaagagat gtacgaccgt
     7621 gctgccgaac agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac
     7681 gaagaacaga cgtcgactac aacggatact ccggcaacgc ctgcacgcgt ctccaccacg
     7741 ctgggtaact gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc
     7801 tgcgcgcggc gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg
     7861 ctgttcgtca ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc
     7921 ccaacactga ctggaatgcg atcttttcgt tatgccaggc catgagtctg tttgccagtc
     7981 gacaaacgct attgctgttg ttaccagaaa acggaccgaa cgcggcgatc aatgaacaac
     8041 ttctcacact caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggtaataaat
     8101 taagcaaagc gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg
     8161 tgacctgtca gacaccggag caggctcagc ttccccgctg ggttgctgcg cgcgcaaaac
     8221 agctcaactt agaactggat gacgcggcga atcaggtgct ctgctactgt tatgaaggta
     8281 acctgctggc gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaac
     8341 tgacattacc gcgcgttgaa caggcggtga atgatgccgc gcatttcact ccttttcatt
     8401 gggttgatgc tttgttgatg ggaaaaagta agcgcgcatt gcatattctt cagcaactgc
     8461 gtctggaagg cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctcttgttac
     8521 tggttaacct gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc
     8581 gggtatggca gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagccgc
     8641 agttacgtca ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattaca
     8701 gtcagtcagt gtgggcagag ttggaagggt tatctcttct gttgtgccat aaacccctgg
     8761 cggacgtatt tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt
     8821 gatccggtgc actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg
     8881 acgcgagtca caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac
     8941 agcgtacagc gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt
     9001 gatgaacgcg agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg
     9061 cggcaggaac aagggccgga cgtgccgctg gcgtttatta ttggtcagga ttcattgctg
     9121 acctttccga cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt
     9181 cggcgtccag gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat
     9241 catttgacac ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct
     9301 gaaacgccgt ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa
     9361 tcatgtgatg atttattgcc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat
     9421 cgctgatacc tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggcgaaa
     9481 cacaagcgaa tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcacgcg
     9541 gtattgagca agcgcaaaat ctgcatacac tgctagatga tgtttccttt gacctggttt
     9601 tatgcagtga actggaacgg gcacagcata ccgcgcgact ggttctcagt gaccgccagc
     9661 tccccgttca tatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac
     9721 atcatcgcga cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc
     9781 agcatgctat ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct
     9841 ttatcgcaag gcttagtgaa tatcagcact atcagaatat tttaatcgtc agccatcagg
     9901 gcgtacttag tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt
     9961 ttcgcgttga ccaggggtgt tggagcgcca ttgatataaa ccaaaaattc gcgacgctac
    10021 gcgtcctcaa tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg
    10081 gtaagccgca acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt
    10141 ttccgccaga cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg
    10201 cggggtggga tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc
    10261 ctctggttca ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac
    10321 ttgcagggta aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag
    10381 gacatcatcg ccttagacgt tcagggcaaa tccagcatca ctgactgcat gatcatctgt
    10441 acgggtacgt ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc
    10501 gcagcgggcc tgttaccgct cggagtagaa ggtgaaaaca gcgccgactg gattgtcgtg
    10561 gatttgggcg atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg
    10621 gaaaaactct ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg
    10681 gactgggtac aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc
    10741 gagctgattg aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc
    10801 gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat
    10861 attccaggca agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg
    10921 gatggtcgcg acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa
    10981 gcggcggcgg agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc
    11041 gtgctggtcg cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac
    11101 cgtgagtgat aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt
    11161 cgcgactata cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg
    11221 attttgctgc tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt
    11281 accgactacc agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc
    11341 cgcggcatta tctacgaccg taacggtatc cctctggccc tcaaccgcac tatctaccag
    11401 atagaaatga tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc
    11461 gtggttgatc tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac
    11521 cgtttcacct ccattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc
    11581 gtcaatcagt accgttttcc gggtgttgaa gttaaaggct ataaacgtcg ttactatcct
    11641 tacggttcgg cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc
    11701 gaacgcctga ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag
    11761 ctgggcattg agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt
    11821 gaagttaaca accgtgggcg tgttatccgc cagttaaaag aagtcccacc gcaagccgga
    11881 cacgatattt acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg
    11941 ggtagccgcg cagctgtggt agtcaccgat ccgcgtacag gtggggtgct ggcgctggtt
    12001 tccacgccta gttatgaccc aaacttgttt gttgacggta tctccagcaa agattattcc
    12061 gccttgttga acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct
    12121 cccgcgtcta cagttaaacc ctatgtggcg gtttcggcat tgagcgccgg ggtgatcacg
    12181 cgcaatacga cgctgtttga ccctggctgg tggcaactgc caggttcgga aaaacgttat
    12241 cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct
    12301 gcggatacct tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg
    12361 atgggtaaat tcggttatgg tcattatacc ggtatcgacc tggcggaaga acgttccggc
    12421 aatatgccta cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac
    12481 accattccgg ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag
    12541 gcactgatga tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc
    12601 gccgaagacg gcaaacaggt gccatgggta caaccgcatg aaccgcccgt tggcgatatt
    12661 cattccggtt actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac
    12721 ggtacggcgc ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atctggtacc
    12781 gctcaggtct tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt
    12841 ttacgtgacc acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc
    12901 gccatgattc tggagaacgg tggcgcgggt ccggcggttg gtacgctgat gcgccagatc
    12961 ctcgaccaca ttatgctggg tgataacaac accgatctgc ctgcggaaaa tcctgcggtt
    13021 gccgcagcgg aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata
    13081 aagtccatct cgatcccaca atgctgctga ttttactggc attgctggtt tacagcgccc
    13141 tggttatctg gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa
    13201 tcgcgatggg tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag
    13261 gctgggcccc ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg
    13321 gtgccatctc taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcagccgt
    13381 cggaaattgc caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt
    13441 gcccgccatc gttgaagaac actggcatcg cgctggtgct gatatttatg cccacgctgc
    13501 tggtggctgc acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg
    13561 tactgttcct ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg
    13621 cgttcattcc gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga
    13681 tgctcctgga cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa
    13741 ttgctattgg ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc
    13801 ttgaatttct ccccgaacgc catactgact ttatcttcgc ggtactggcg gaagagctgg
    13861 gattagtggg cattttgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt
    13921 ggatagccgc cagagcgcaa accacctttg gtcgcgtcat ggctggcggc ttaatgctga
    13981 tattattcgt ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag
    14041 gggttccgct cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt
    14101 tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg
    14161 tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt
    14221 acaagcgatg atggtcagca acagacagta agtgtaccgc agcctgcggt atgtaacggc
    14281 cccatagttg aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat
    14341 caggattacc agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttatc
    14401 caggcgggac tggcggcaat ctatgatgcc gaaccaggca gtaacctgac ggcctctggc
    14461 gaagctttcg atccgacaca gctgacggcg gcccatccaa cgcttccgat ccccagctac
    14521 gccagaatca ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggt
    14581 ccttacggca acgaccgcgt tatttcgctt tctcgcgcag cagctgaccg tcttaacacg
    14641 tcaaacaaca ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct
    14701 ggtcctggta tggcttgtac cacagtcgcc aaacagactt acgccctgcc tgcacctccc
    14761 gatttaagcg gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg
    14821 gtcagtaatt cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagcagcggt
    14881 ttcctcggcg caccaacgac cttagcgcca ggtgtactgg aaggcagcga accgacgcct
    14941 gctccacagc ccgttgttac agctccgtcg acaacgcctg caacctcgcc tgcaatggtg
    15001 acaccgcaag ccgcctcgca aagcgccagc ggcaacttta tggtgcaggt cggggccgta
    15061 agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc
    15121 ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccaacaaa
    15181 gccgaagcca gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt
    15241 accaccgcgc agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa
    15301 caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt
    15361 ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat
    15421 catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc
    15481 cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta
    15541 catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg
    15601 cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc
    15661 cggtaaattt aaagaaactg atttagtcac tattggcaac gacgcatggg ccaccggtaa
    15721 cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc
    15781 tcagctgatc cgcggtataa acctgcaatc gggtaacgat gcttgtgtcg ccatggccga
    15841 ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgccct
    15901 gggcctgaaa aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag
    15961 ctccgcgcgc gatatggcgc tgatcggcca ggcgttgatc cgtgacgtac cgaatgaata
    16021 ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg
    16081 cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc
    16141 aggttacaac cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat
    16201 gggcggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt
    16261 ccgtttcttc gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt
    16321 ttggtttggt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat
    16381 tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca
    16441 tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac
    16501 gatcgaacaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa
    16561 aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg
    16621 aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaactcccgc cttctggcgg
    16681 gagttgctat ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc
    16741 ttgaattccc tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg
    16801 atcaggtggt tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac
    16861 caagcagcaa aggcaactac cactcggtat ctatcactat caacgccact catatcgagc
    16921 aggttgaaac actgtatgaa gaactgggca aaatcgatat tgtccgcatg gttctgtaac
    16981 tcgcttctcc gttacccggc ccctggtcgg gtaactcccc tctcctcgct gtgatatact
    17041 tttctcccac ttttactcat tctccacgga gatgccgttt tgtatcagga taaaattctt
    17101 gtccgccagc tcggtcttca gccttacgag ccaatctccc aggctatgca tgaattcacc
    17161 gatacccgcg atgatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc
    17221 acccaagggc aggcaggaaa ggcggagcac attttaatgc ccggtgatat tccggtgatt
    17281 cagagcgatc gcggtgggca ggtgacttat cacgggccgg ggcaacaggt gatgtatgta
    17341 ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa
    17401 acagtggtga ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcca
    17461 ggtgtctatg ttggggaaaa gaaaatttgc tcactgggtt tacgtattcg acgcggttgt
    17521 tcattccacg gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat
    17581 ccttgtgggt atgccggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg
    17641 actaataata ttgcaccacg tttactggaa aatattttag cgctactaaa caatcctgac
    17701 ttcgaatata ttaccgctta atttcatacc tcaatggccc aatttacact gggtcattac
    17761 tcataacacc tgctaatgct ttacatctcc ttcgcgtttg tatattttct ttatcacttg
    17821 cagaaatgcg atgctacgcc ttgctccgta actcacccca ttgattaact tattgaaata
    17881 gttgattatg atgagcaaaa gcgttccgtt ccaattagac ataagattat tgtaattttt
    17941 ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta ataatcaaat
    18001 tgaatcctgt ttaagcagaa aatcgtcaga aggtaaacca caaatattta caaccttacg
    18061 aaatattgat cttaatcttc tgactatttt tgaagctgta tatgtacata aagggatcgt
    18121 taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta ttcagaaact
    18181 gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca ctcctaccgc
    18241 atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc ttggcgcgct
    18301 ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca ccccctcggt
    18361 cggagctctg gtccttcctg tcatttatcg ggcgattaaa actcactatc cgcagctttt
    18421 actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc aaaccgatct
    18481 catcatagat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc tgttcaccga
    18541 caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag atgacagaga
    18601 gactatcgac aacgctgcac atgtactcct gttaccggaa ggacaaaatt tcagcggtct
    18661 gcggcagaga attcaagaga tgtttccgga ccggcagatc agcttcacca gctacaacat
    18721 tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc ccagccgttt
    18781 ttataacctg tttagccgtt gctggccgct ggaaaaactg ccttttccgt ccttaaatga
    18841 ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc ccatcctgca
    18901 cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag ccagatcaca
    18961 gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg accgctttgg
    19021 ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa tagttgataa
    19081 ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg aacacgcacg
    19141 ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc gataagatgg
    19201 cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc aagccggaat
    19261 ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa gccgcaatgc
    19321 gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg gcggaatgct
    19381 tcaaccacgg cacagcaacg tttatgatcc tgggcgctat ttgtacccgc cgttgtccgt
    19441 tctgtgacgt tgcccacggt cgcccggtag ctcctgatgc caatgagcca gtgaaactgg
    19501 cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt gaccgtgatg
    19561 acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt cgggaaaaaa
    19621 gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgtatg gatcgtgctc
    19681 tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa aacgtaccgc
    19741 gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag ctgctggaac
    19801 gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg ggtctgggtg
    19861 aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt gtgacgatgt
    19921 taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa cgttacgtta
    19981 gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt acccatgctg
    20041 catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa gggatggaag
    20101 ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct ttttgaagcg
    20161 ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc actctttatg
    20221 agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag catcgtcatc
    20281 attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca gcgtacgtaa
    20341 cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt tggtaatact
    20401 aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc cttaaaaacc
    20461 aaagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac aaggtgaatc
    20521 aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg gttgtttaag
    20581 acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc cataatgagt
    20641 gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat ccggctttgc
    20701 ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt atccagcgca
    20761 cgagcggcaa gcaacgttga ccagtggtgc tctttgagcg gcccgcgaac ccaggcggca
    20821 ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc tggaaagcgt
    20881 aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa cggagcaatt
    20941 tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata gagatgcaat
    21001 ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt ccatgcgcgc
    21061 cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg tttactttct
    21121 cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac cgatagatct
    21181 gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga tacgtcgttt
    21241 tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca cacagatgta
    21301 acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg taaaattcgc
    21361 caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc
    21421 agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg tggcgagatg
    21481 gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga cgctggcagc
    21541 aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca ggatgacgaa
    21601 cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt
    21661 ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcatt
    21721 actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg cattctggct
    21781 gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg
    21841 aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc
    21901 gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg
    21961 gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa
    22021 tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt
    22081 tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg tggtacgctt
    22141 tgtccagtgc aactttacta aaaagtgata aaaatcgcgt tatttttgat gttgcttatt
    22201 ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact gaaagcgcat
    22261 ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaaagt
    22321 aactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga tgtaattcca
    22381 gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat tttcatccgc
    22441 cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga
    22501 gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc aacgcgacag
    22561 gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc
    22621 gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag gctgttgcca
    22681 ggtttgtaca atattttctc taaacgttgt catccactca tctgcgttag caaaaacttt
    22741 accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt tactcacgtt
    22801 catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa gctacaatta
    22861 acataacctt aacagcccca acataaagaa taatctgaat agctgctcta tttatttccg
    22921 cttgttcaag ttaaatattt ccgcgcatcg atttaaaatg acacatcgtt ttctgcgatg
    22981 ggaatagtca taaaagaaaa agctagttgt ttctgacaac aaaatgcaac caagaaaaaa
    23041 tgacttattt tttaataaga taccaaaaat catcttaaca gggaatatat tatatatcgt
    23101 caacccacgc ccattgcaga ttattttttg agcacacaac cgcagctaac accattgcaa
    23161 ttaacaaatt tgcatcaatc ctccatcaat ttacacacat tattatgtga taattgccaa
    23221 ccgctaaata tgcgttttgt tatctacgta taaaaacagc aacttcaatg tcttaatggc
    23281 agtttttctt gattttaatc agcattcatc gccaatttat tgggcatatt ttttccttaa
    23341 gctttaggaa ttttttatta tttactttgg ggcctggaga caggaaaaat tatgctgaca
    23401 ttcattgaac tccttattgg ggttgtggtt attgtgggtg tagctcgcta catcattaaa
    23461 gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat tatcagtgcc
    23521 attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag cgccacggat
    23581 atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg catgatgatt
    23641 atgatgctgt gtggatttgc cgcttacatg acccatatcg gcgcgaatga tatggtggtc
    23701 aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat gattgccgcc
    23761 tattttgtcg cctgtctaat gtctctggcc gtctcttccg caaccggtct gggtgttttg
    23821 ctgatggcaa ccctatttcc ggtgatggta aacgttggta tcagtcgtgg cgcagctgct
    23881 gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga tgtggtgctg
    23941 gcggcgcaag cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac gctgcctatc
    24001 tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg ttatctggat
    24061 aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac cactgcccct
    24121 gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt tgacggtaaa
    24181 tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat tgcctccatt
    24241 ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga agtggcttat
    24301 cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc tggggtattc
    24361 gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc tacctcgttt
    24421 ggttcggcga gtatcatcct gatgctggta ttggtgattc tgacaatgct ggcggcagtc
    24481 acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc gaaactggcg
    24541 cactcttccg gcattaaccc ggcgtatttg actatcccga tgctgcaggc gtcaaacctg
    24601 ggccgtaccc tttcgcccgt ttctggcgta gtcgttgcgg ttgccgggat ggcgaagatc
    24661 tcgccgtttg aagtcgtaaa acgcacctcg gtaccggtgc ttgttggttt ggtgattgtt
    24721 atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg caagtaaata
    24781 gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg tatatgcgtt
    24841 gcggtctgcc aactttaccg tgaacaattt cagcaataat cagatggcgg ctggcgcaat
    24901 attcaagata acgcctggca gtggtgcgac tgatggtcag tgcctgcgcc accgtttctg
    24961 ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc gtcaaggggt
    25021 caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta aacatctcat
    25081 caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc tggcggaaac
    25141 gggttagcgt ttgccccagc cgttcatagg caatgggctt aatgagataa tcaaatacac
    25201 cacaacgtac ggcttcagac accgtttcca tatcgctggc tgcagtggta aacaccacgt
    25261 cgccgggata atgcgcctgc accagttcat gcagtaaatt aatccctcta ccgtcaggaa
    25321 gatagttatc gagcaagatt agccccggct taaaacgctc gatcatcatt cgggcctgcg
    25381 ccaggtttcc cgccagtaat atctgactga atccgggaat gtgacgaata tattcagcat
    25441 gcatctctgc cagcggcgtt tcgtcctcaa cgatcaatag ggttaatgga gctgtcattt
    25501 ggtttcactt tcggaatata gattgaaaat aaggtaccgc agggatcatt atcttcgaga
    25561 gtgataacac caccgcagcg cgttacgtag ctggcaatca agtacaaccc aatgccatgt
    25621 tcaccgggct cgtcagcacg cgtactgacc ccctgctcaa atattttgtc tcgtagagac
    25681 tctggaacgc cgcagccctg atcggcgact tcaatcacca catcatcgcc ttcatcgctg
    25741 aggaataatt caacgatctt gtttccttca tcgctacgca ggctggcttc gaaggcgtta
    25801 tcaagtaaat tgcccacaat ggctgcaaac tcggtgctat ccagtcctgg cggcagttgc
    25861 gaaagctggc taccggggac aatgatcatt tttagcccca gttcccgggc gcgctgcact
    25921 ttaccaaaaa gcagccccgc cacctggcga tcggcaaacg cctcgcgcag gctgtcaata
    25981 agctgttgct gggcctgaga ctccccctgc accatcgcca gcacgcgatc atactctttc
    26041 atctgcaaca gaccattgag cgtcgacatc caattgagat gctcgtgtcg caatgtacga
    26101 aggctctcaa cgtattgttt tatttgcgtc agttgcgcat tgagggtgga aatttcgtct
    26161 ttactgcgaa agctgataat ggcccccagc aaatcatcac ctgaacgaat agcttcccga
    26221 ttggcaataa cgctcagacc gttaaagtta gccactacat cctgacgttt ttcatcaatc
    26281 tgttcggtaa agaaatcggc gggcctgacc acttcagcaa tgggtttacc caaccattgc
    26341 cgtccgggag agctcagccc cagcatcttt cttgcgttac gattgatggc ggtaatgtaa
    26401 ccatgcggat ccaccgcaat cagcccttca taaaccgaac taaacagcgc ctcttgctga
    26461 cggaccacgc gtgcgatttg ctttggctcc atgcccatca tttgccgacg gatatgcgct
    26521 gccaggaacc acgacagcaa catcagaatc cctaacagca cgacaaacac gcctgccatc
    26581 ggtaataaaa actcagcccg ccagctatcg attttactca ccaggtagcc aatcgacacc
    26641 acgccgatga cttttccatc gtcatcaaag attggcgttt tggcgcgcat cgccatcccc
    26701 attgaccctt tcccggtaat gaagtagctc tcccctttct ccagcgcgcc ctgtttggtg
    26761 aactgcatag gataaccaat tttctccgga ttaggatggt aaaggcggat cgagtgccgg
    26821 tccccaatca ccacataatc aaaatcggta tctctttgta atttgttagc gatggtcgcc
    26881 agccgtttgt agtcacgcgt tttcacctca gagatgacac tgtcattgga ggcaataatt
    26941 ttcgcctgat tcattgccat gtcgcgtaca tgaagcgtta aatagtcctc aaaactggcc
    27001 gtaaaatatt gcgccagggc tgcaatgaca aatattgaga acaccagaat cagcaaaaag
    27061 atacgcagcg gaaatgccag tctttggaaa aatgcaaact gtttattctc gttaagctgc
    27121 aacattgttt tccttgcggg ttgtcccgag cctctttgac ggat
//
LOCUS       DAZFTN010000064        27108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFTN010000064 DAZFTN010000000
VERSION     DAZFTN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(166..1830)
                     /gene="glnS"
                     /locus_tag="ACHKV2_002970"
     CDS             complement(166..1830)
                     /gene="glnS"
                     /locus_tag="ACHKV2_002970"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO6940823.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2033..3979)
                     /gene="nagE"
                     /locus_tag="ACHKV2_002971"
     CDS             complement(2033..3979)
                     /gene="nagE"
                     /locus_tag="ACHKV2_002971"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO6940824.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4312..5112
                     /gene="nagB"
                     /locus_tag="ACHKV2_002972"
     CDS             4312..5112
                     /gene="nagB"
                     /locus_tag="ACHKV2_002972"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6940825.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5172..6320
                     /gene="nagA"
                     /locus_tag="ACHKV2_002973"
     CDS             5172..6320
                     /gene="nagA"
                     /locus_tag="ACHKV2_002973"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6940826.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6329..7549
                     /gene="nagC"
                     /locus_tag="ACHKV2_002974"
                     /gene_synonym="nagR"
     CDS             6329..7549
                     /gene="nagC"
                     /locus_tag="ACHKV2_002974"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO6940827.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7597..8349
                     /gene="nagD"
                     /locus_tag="ACHKV2_002975"
     CDS             7597..8349
                     /gene="nagD"
                     /locus_tag="ACHKV2_002975"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO6940828.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8606..10270
                     /gene="asnB"
                     /locus_tag="ACHKV2_002976"
     CDS             8606..10270
                     /gene="asnB"
                     /locus_tag="ACHKV2_002976"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO6940829.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRYVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10650..10726
                     /locus_tag="ACHKV2_002977"
     tRNA            10650..10726
                     /locus_tag="ACHKV2_002977"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10684..10686,aa:Met,seq:cat)
     gene            10737..10821
                     /locus_tag="ACHKV2_002978"
     tRNA            10737..10821
                     /locus_tag="ACHKV2_002978"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10771..10773,aa:Leu,seq:tag)
     gene            10845..10919
                     /locus_tag="ACHKV2_002979"
     tRNA            10845..10919
                     /locus_tag="ACHKV2_002979"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10877..10879,aa:Gln,seq:ttg)
     gene            10954..11028
                     /locus_tag="ACHKV2_002980"
     tRNA            10954..11028
                     /locus_tag="ACHKV2_002980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10986..10988,aa:Gln,seq:ttg)
     gene            11044..11120
                     /locus_tag="ACHKV2_002981"
     tRNA            11044..11120
                     /locus_tag="ACHKV2_002981"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11078..11080,aa:Met,seq:cat)
     gene            11169..11243
                     /locus_tag="ACHKV2_002982"
     tRNA            11169..11243
                     /locus_tag="ACHKV2_002982"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11201..11203,aa:Gln,seq:ctg)
     gene            11281..11355
                     /locus_tag="ACHKV2_002983"
     tRNA            11281..11355
                     /locus_tag="ACHKV2_002983"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11313..11315,aa:Gln,seq:ctg)
     gene            complement(11509..12684)
                     /gene="ubiF"
                     /locus_tag="ACHKV2_002984"
     CDS             complement(11509..12684)
                     /gene="ubiF"
                     /locus_tag="ACHKV2_002984"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO6940830.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12830..14254
                     /gene="miaB"
                     /locus_tag="ACHKV2_002985"
     CDS             12830..14254
                     /gene="miaB"
                     /locus_tag="ACHKV2_002985"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO6940831.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14407..15447
                     /locus_tag="ACHKV2_002986"
     CDS             14407..15447
                     /locus_tag="ACHKV2_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO6940832.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15444..15911
                     /gene="ybeY"
                     /locus_tag="ACHKV2_002987"
     CDS             15444..15911
                     /gene="ybeY"
                     /locus_tag="ACHKV2_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO6940833.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16001..16879
                     /gene="corC"
                     /locus_tag="ACHKV2_002988"
     CDS             16001..16879
                     /gene="corC"
                     /locus_tag="ACHKV2_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO6940834.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16904..18442
                     /gene="lnt"
                     /locus_tag="ACHKV2_002989"
     CDS             16904..18442
                     /gene="lnt"
                     /locus_tag="ACHKV2_002989"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO6940835.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18839..19747
                     /gene="gltI"
                     /locus_tag="ACHKV2_002990"
     CDS             18839..19747
                     /gene="gltI"
                     /locus_tag="ACHKV2_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO6940836.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19917..20657
                     /gene="gltJ"
                     /locus_tag="ACHKV2_002991"
     CDS             19917..20657
                     /gene="gltJ"
                     /locus_tag="ACHKV2_002991"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO6940837.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20657..21331
                     /gene="gltK"
                     /locus_tag="ACHKV2_002992"
     CDS             20657..21331
                     /gene="gltK"
                     /locus_tag="ACHKV2_002992"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO6940838.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21331..22056
                     /gene="gltL"
                     /locus_tag="ACHKV2_002993"
     CDS             21331..22056
                     /gene="gltL"
                     /locus_tag="ACHKV2_002993"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO6940839.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22174..23109
                     /gene="rihA"
                     /locus_tag="ACHKV2_002994"
     CDS             22174..23109
                     /gene="rihA"
                     /locus_tag="ACHKV2_002994"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO6940840.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23193..24863
                     /gene="hscC"
                     /locus_tag="ACHKV2_002995"
     CDS             23193..24863
                     /gene="hscC"
                     /locus_tag="ACHKV2_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO6940841.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24923..26374)
                     /gene="djlC"
                     /locus_tag="ACHKV2_002996"
                     /pseudo
     CDS             complement(24923..26374)
                     /gene="djlC"
                     /locus_tag="ACHKV2_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26371..27078)
                     /locus_tag="ACHKV2_002997"
     CDS             complement(26371..27078)
                     /locus_tag="ACHKV2_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6940842.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6653 a         7040 c         6883 g         6532 t
ORIGIN      
        1 gtgaacgcct tatccggcct acaaaagcac gcaaactcaa tatattgcag agatcatgta
       61 ggcctgataa gcgtagcgca tcaggcaatt tagcgtttga aactgtttgt ctgttccttg
      121 ccggatgcga cgtaaacgcc ccatccggca tagcgaaact taaaattact cgcctacttt
      181 cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttccg ccgtagaatg
      241 gcggctatcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac ccgcaaccgc
      301 atctttcagc gacggttcag caaagccctg tttgatcacc agcgattccg ggttaatcac
      361 cgacaggaaa tcatccgcag cacctgggtt cggcacgctg aacagacggt catacaaacg
      421 gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt tgactttacg
      481 accatctgcc ggatctttgc ttaaggtatc ggcgtcataa gtacagaaga tggtggtgat
      541 attaccttcg gcatctttct cgacgcgttc tgccttaatc acataagcat tacgcagacg
      601 cacttcttta cccagcacca gacgtttgta ctgcttgtta gcttcttcgc ggaaatcgga
      661 gcgatcaatc caaatctcac cgctaaacgg cacctgacgg ctccccattt ccggtttgtt
      721 cggatggttc ggcatggtaa ccatttcgcc ttcgccctga tagttttcga taaccagttt
      781 caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg ttgagatctt cacggatgca
      841 ggattccagc gacgccatct caatggtgtt gtcctgcttg gtcacgccga tgcgtttgca
      901 gaactcacga atagaagccg cagtgtaacc acgacgacgc agaccggaaa tggtcggcat
      961 acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaagttca gcttacgctt
     1021 ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg ggtgaacagg
     1081 aatggtgatg ttgtccagta cccagtcgta cagacgacgg ttatcctgga actcaagcgt
     1141 acacagagag tgcgtaatac cttccagggc atcgctgatg cagtgggtga agtcgtacat
     1201 cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa tgcggtacag
     1261 caccggatcg cgcatcacga taaacggtga agccatgtcg attttcgcac gcaggcaggc
     1321 tttaccttct tcaaaaccgc cggtacgcat tttctcgaac agcgccaggt tctcttcaac
     1381 gctacggtcg cggtacgggc tgtttttacc aggctgcgtc agggtgccgc ggtattcgcg
     1441 gatctgttcc ggcgtcagtt catcaacgta cgccaggcct ttattgatca gttcgatcgc
     1501 ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag accagtgaaa
     1561 acctaaccac tcaacgtcgt ttttgatcga atcgacatac tcgatatctt cttttaccgg
     1621 gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga tcccgaagtt
     1681 caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg gcgggaaacg
     1741 ggtgtgtact gtggtgtgct taccactggc cagatcttca tcgatgatct gacggataaa
     1801 gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta aacaacgtat
     1861 aacggcgtat gatcttataa gcgggacagg ctgacaactg ttagttagtg aaaatcgaaa
     1921 atctcactga gattatgggg atgattgatg aaaaaaaaag cggcagagaa gatctccacc
     1981 gcttgtcatt gttggatgcg acgctcaagc gtcgcatcag gcataaagca gattactttt
     2041 tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta atgatcaagc
     2101 cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt
     2161 tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc gcgccctctt
     2221 ccaccagacg tttaaagcct ttaccttcca gcgctacggt atcgataccc atgtgaacga
     2281 caatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg aagattttca
     2341 cgattgttcc tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt
     2401 cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc acgacatcac
     2461 cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg
     2521 ctacaggagc ggcagttgcc ggagttgctt cagcagaggc agctgctacc gggccacggg
     2581 caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca atcacctgaa
     2641 tagtctgttt gttcagtttt actaccccag aagcccccag acgtttacac atcgtatcgt
     2701 taacgcgggc agagtcagcc acggtaaggc gcagacgggt gatacaggcg tcaattgctt
     2761 tcaggttgtc agtgccgcca accgcagcaa tatagttggt cgccagttga ttgaggcctt
     2821 cttcagtgtt gctgttggct tcttcagtaa cgatctcgtc ttctttatct tcacgacccg
     2881 gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga
     2941 agacaacacc cataaccagc agcatccaga cgttctggct ggcggccggc aggttataca
     3001 tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc ggcatggata cccagcagcg
     3061 ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa
     3121 ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa
     3181 cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg
     3241 ccagcgccgc acccggcagg ccgaacatca tgatcgggaa gaagccggac atgaacatcc
     3301 ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg
     3361 cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg
     3421 ttgggatcaa cagacggttg atgaaaccaa agataccgga acccagcgcg cccgcagaaa
     3481 cgatccactc gccgcctgca tggatagcgt gctgtaccgg cggccagacg taaccaaaaa
     3541 tggccgccag caccagacag aagaagccgg tggcgatcgg cacaaagcgt ttgccgccga
     3601 agaagctcag gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa
     3661 ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca
     3721 tcgctttggt taacacaaag taacctaccg cccccgccag cgccgccgca cctgcgctgt
     3781 ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg
     3841 caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca
     3901 acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga
     3961 aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc
     4021 cgtattttta ttgtgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca
     4081 tctgccacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgagt gtcttttgtg
     4141 agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaacc agaaaactta
     4201 ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct
     4261 taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg
     4321 atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt
     4381 atcaatgcgt tcaaaccgac tgctgatcgt ccgtttgttc tgggcctgcc aactggcggc
     4441 acgccaatga ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt
     4501 aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa
     4561 agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac
     4621 atcaaccttc tcaacggcaa cgccccggat atcgacgccg agtgccgcca gtatgaagaa
     4681 aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat
     4741 attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact
     4801 catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa
     4861 tatgccctga ctgtcggtgt tggtacactg ctggatgccg aagaagtgat gattctggtg
     4921 ctgggtagcc agaaagcact ggcgctgcag gccgcagttg aaggttgcgt gaaccatatg
     4981 tggaccatca gctgtctgca actgcatccg aaagccatca tggtgtgcga tgagccttcc
     5041 accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc
     5101 aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat
     5161 cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg
     5221 atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac
     5281 tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg
     5341 atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg
     5401 tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc
     5461 cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt
     5521 acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggcccg tggctgaatc
     5581 tggtgaaaaa aggtacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg
     5641 atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc
     5701 ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca
     5761 acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc
     5821 tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg
     5881 atgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca
     5941 ttcgtaatgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc
     6001 cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg
     6061 gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg
     6121 tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc
     6181 tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag
     6241 tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc gttaacggta
     6301 acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa
     6361 tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg
     6421 gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa
     6481 aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac
     6541 cgggggccgc cgcgctatct ctatcgtcac cgaaacccgc aatttccacg caatcggcgt
     6601 acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc
     6661 agaagaacat tacccgctgc cggaacgtac ccagcagacg ctggaacatg ccctgctgaa
     6721 tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga tcgccatttc
     6781 cgttatcctg ccaggacttg ttgacccgga cagcggcaaa attcattaca tgccgcatat
     6841 tcaggtagaa aactgggggc tggtagaagc actggaagag cgttttaaag tgacctgttt
     6901 cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga
     6961 ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa
     7021 cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa attggccata ttcaggtcga
     7081 accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgctgccaa
     7141 cgccgctatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc
     7201 gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagcctggc
     7261 ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa accatcgcca ttgctatcaa
     7321 cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct
     7381 gctccctgct attgaaagct gcattaatac tcaggcgctg aaggcgtttc gtactaatct
     7441 gccggtggta cgttctgagc tagaccaccg ctcggcaatc ggtgcttttg cgctggtaaa
     7501 acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat
     7561 agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc
     7621 gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg
     7681 cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc
     7741 gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtacctga cagcgtgttt
     7801 tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcgtat
     7861 gttgtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat
     7921 gtgaaccctg attttgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat
     7981 aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac
     8041 gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag ggattgagaa aatctccggg
     8101 cgcaaaccgt tctatgttgg taagccgagt ccgtggatta tccgcgcggc attaaacaaa
     8161 atgcaggcac attcggaaga aacggtgatt gtcggcgata acctgcgtac cgatattctg
     8221 gccggcttcc aggcaggtct ggagacgatt ctggtgcttt ctggtgtttc gtcgctcgac
     8281 gatatcgaca gtatgccttt ccgccccagc tggatttacc cgtcagtcgc tgaaatcgac
     8341 gttatctgaa tagcaaccac gtctcaggac gtggtttctt atttttaccc atcattaaaa
     8401 tcatcccatt caataaaacc cgccaaaaat tatcgatcca tcaataaata catcaccgca
     8461 ccctcccctt tttcacaaat cagcaatcac cattacgttt tttatttttt caccgccaaa
     8521 tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac aacaagcaac
     8581 aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg atatcaaaac
     8641 agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg
     8701 cccggactgg tccggtattt atgccagcga taaagccatt ctcgctcacg aacgtctgtc
     8761 aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa cccacgtact
     8821 ggcggtaaac ggtgaaatct acaatcacca ggcattgcgc gctgaatatg gcgatcgtta
     8881 tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc
     8941 ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga
     9001 tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg gctatgacga
     9061 acacggtcag ctgtatgtgg cctcagaaat gaaagccctg gtgccagttt gccgcacgat
     9121 taaagagttc ccggcgggga gctatttgtg gagtcaggac ggcgaaattc gttcttatta
     9181 tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca aaaacgagct
     9241 gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt
     9301 gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga aatacgcagc
     9361 ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac actcctttgc
     9421 tgtaggtctg ccgggttcac cggatctgaa agcagcccag gaagtggcaa accatctggg
     9481 cactgtgcat catgaaattc acttcactgt acaggaaggt ctggacgcca tccgctacgt
     9541 gatttaccac atcgaaacct atgatgtgac cactattcgc gcttcaacac cgatgtattt
     9601 aatgtcgcgt aagatcaaag cgatgggcat taaaatggtg ctgtccggtg aaggttctga
     9661 tgaagtgttc ggcggttatc tttacttcca caaagcaccg aatgccaaag aactgcatga
     9721 agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgtg ccaacaaagc
     9781 gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaagaaat tcctcgacgt
     9841 ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaagatgg aaaaacacat
     9901 cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc agaaagagca
     9961 gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg cggcgcagca
    10021 ggtttctgat caacagctgg aaactgcccg cttccgcttc ccgtacaaca caccgacctc
    10081 aaaagaagcg tatctgtacc gggagatctt tgaagaacta ttcccgcttc cgagcgccgc
    10141 tgagtgcgtg ccgggcggtc cttccgtcgc gtgttcttcc gctaaagcga tcgaatggga
    10201 tgaagcgttc aagaaaatgg acgatccgtc tggtcgcgcg gttggtgttc accagtcggc
    10261 gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca gttattgagg
    10321 tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat cactgtttga
    10381 caactcatca ctgtaggcca gtatttcata gattaatttc ccaccactat tgttaaattc
    10441 cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg ggaaaaaaca caaagtaaac
    10501 aataattgac gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc
    10561 aattttcctt gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa
    10621 cgccgataag gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc
    10681 actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt ttttttgcgg
    10741 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    10801 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    10861 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    10921 cttctttgag taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc
    10981 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa
    11041 tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    11101 gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa attcaaaaac
    11161 caatttgttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg cattccgagg
    11221 ttcgaatcct cgtaccccag ccaatttatt caagacgctt accttgtaag tgcacccagt
    11281 tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc
    11341 ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc
    11401 actcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg cgacgctgcc
    11461 gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct acaaccctaa
    11521 cgcatatttc agcgcctgac gtttcaacac gccagcacgc tccgccgcca tcaaaccaag
    11581 attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa acagatccat
    11641 accgctctgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct tgaggacagg
    11701 ataactggcc cacgcttcgc cgtagctgcg ggcattcacc agaacatcaa tcagggcatc
    11761 gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg tgtgcgcggc
    11821 atcacccacc agcgcaagcc ccggctgcac atactgcaac gcatggcggc gtgtcagcgg
    11881 aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat gcttcgcgat
    11941 ttccacctgt aactgcgcca tattcatatt ctgcaactgg cgaatgcgcg ccggagagtc
    12001 gtaccacacc agcgatgccc agttatcaaa cagcggcaga aacgcgcgcg gtccatccgg
    12061 ggtaaattgc tgccaggtgc tatcgccggg atcgttctcg cactggacgc taatcaacat
    12121 gcacgactgc gcatactgcc atgcatgaac gccaattccc gccatctgcc gcacctgcga
    12181 atttgcgccg tcggcaccaa tcaccagctt cgcgcgaatc acttcaccgc ctttcagctc
    12241 cagctcctga agatcattat ctcgatgtaa tgcaatcagc gaggttggca cacgtaacgt
    12301 tactttcgga tgcgcttcca gcgcctgcca caatgcctgt tgcaggacag tgttttctac
    12361 catatagcca agcaacggca actttaattc ggctgcgtca aataccacat gcgccgtttc
    12421 ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta ctgcatccca
    12481 gacccctaac cctttaagca atgataccga agccgcgctg atcgccgaga tccgcacgtc
    12541 cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg ttaccgtaaa
    12601 tccgtgctgt gccagcccca gcgccagtgc gccgccgacc attcctccgc cgacaatggc
    12661 aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc tttaagttta
    12721 caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc agcaaagcat
    12781 tacactatgc gccctgcatt cctggctact atttcgcaag agcaagtcga tgaccaaaaa
    12841 actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga agatggccga
    12901 tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag cggatgtgct
    12961 gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg
    13021 tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg gtggctgcgt
    13081 ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata ttatttttgg
    13141 gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg accgcagccc
    13201 ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctaccgg aaccgcgcgc
    13261 cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt gcacctactg
    13321 cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata ttctgtttga
    13381 gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc agaacgtgaa
    13441 cgcctggcgt ggtgaaaact acgacggcac caccggatcg tttgccgatc tgttgcgtct
    13501 ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc cgatcgaatt
    13561 caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct tcctgcatct
    13621 gccggtacag agcggttccg atcgcattct gaacctgatg ggacgtaccc atacggcgct
    13681 ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccagatattc agatcagttc
    13741 tgacttcatc gtcggcttcc ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct
    13801 gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac gtccgggtac
    13861 accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcagc gtctgtatat
    13921 tctgcaagaa cgtatcaatc agcaagcgat ggcgtggagc cgccggatgc tcggcaccac
    13981 ccagcgtatt ctggtagaag gtacatcgcg taagagcatc atggagcttt ccgggcgtac
    14041 ggaaaataac cgcgtggtca acttcgaagg cacgccggat atgatcggta aattcgtcga
    14101 tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc gtactgaaga
    14161 tgaaatgggt ctgcgcgtgg cagaaacacc agaatcggtg attgcccgta ctcgcaaaga
    14221 aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt tccggtgggc
    14281 cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc
    14341 gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac agataagagg
    14401 aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa cgcgcgtctg
    14461 ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg tctcggcatc
    14521 gagatcaatc gccgcgataa ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct
    14581 gcggcagaca ttctgcgtag cctgtatgtc gatactgccc cgatgcgcgg tcagattcag
    14641 gatatcgaac cggaacagat ccaccttgcg attaaagaag cacgggtact ggagcaaagc
    14701 gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt
    14761 aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca tgacattacc
    14821 ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc ggcagttgat
    14881 gccctggagc gtcaggaaat tcgccgtatt ctgctgactc gtccggcagt agaagccggt
    14941 gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta tctgcgcccg
    15001 ctgtacgacg cgctgtttga aatgctgggt tttgagaaag tcgagaaact gattgagcgc
    15061 aacgttattg aagtcgcacc gctggcctat atgcgtggtc gtacgctgaa cgacgcattt
    15121 atcattctcg atgagagcca gaacactacc atcgaacaga tgaagatgtt cctgacccgt
    15181 atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga cctgccgcgt
    15241 aatactaaat caggcttacg tcatgccatc gaagtgctgg ccgatgtcga agagatcagc
    15301 tttaacttct tccacagcga agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac
    15361 gcctatgaag cctgggaaga agccgaacaa aaacgtaaag cggcgctggc ggcagaacgc
    15421 aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg
    15481 aagataactc cggattgccg gaagagagcc agtttcaaac atggctgaat gcggtgatcc
    15541 cgcagtttca ggaagagtcg gaagtgacga ttcgcgtggt cgataccgcc gaaagccaca
    15601 gtctcaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg
    15661 aagtgccgcc tggcatggag atgtcgctac tgggcgatct ggttatctgc cgtcagatgg
    15721 ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg catatggtgg
    15781 tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa gcagaagaaa
    15841 tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg tacattgccg
    15901 agaaagaata atttgccagc catttgactg gcagacgtcc ccgccgttgg tgattaacga
    15961 cggcgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg acaattcaca
    16021 cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca gccaactttt
    16081 ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt ccgggcagaa
    16141 cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca
    16201 acgcgtccgc gacatcatga tcccccgctc ccagatgatt accctgaaac gcaaccagac
    16261 gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc cggtgattag
    16321 cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc cgtttatgcg
    16381 cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg tcgttcctga
    16441 aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc acatggcgat
    16501 cgttattgac gaattcggtg gggtttccgg tctggtaacc attgaagaca tcctggaact
    16561 gattgttggt gagattgaag acgaatatga cgaagaagat gatatcgact tccgtcagct
    16621 gagtcgtcat acctggaccg tgcgcgcact ggcttccatt gaagacttca acgaagcgtt
    16681 cggcacccac tttagcgatg aagaagtcga cactatcggt ggtctggtga tgcaggcatt
    16741 tgggcatctt ccggcacgtg gcgaaaccat cgacatcgac ggttaccagt tcaaagtggc
    16801 gatggccgac agtcggcgta ttattcaggt tcatgtcaaa atcccggatg actcacccca
    16861 gccgaagctg gatgaataaa accgaaactg gatagataac tacatggctt ttgcctcatt
    16921 aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct gcggaacgct
    16981 ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc
    17041 gttgaccttt aaccgccgtc cactccagtc tgccgctatt ggcttttgct ggggatttgg
    17101 cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg gcggaatgcc
    17161 tggcccggtt aacatcttcc tggtggtact gctggcggcg tatttgtcgc tgtataccgg
    17221 actgtttgct ggcgtgctgt cgcgcctgtg gccgaaaacc acctggctgc gcgtggcgat
    17281 tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtac tgacaggctt
    17341 cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc tggcaccgct
    17401 aatgggcgtg gaagccatta acttcctgct tatgatggtt agcggcctgc tggcactggc
    17461 gttagtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg cccttccctt
    17521 cccgctgcgt tacatccggt ggtttacccc acaaccggag aaaaccattc aggtttcgat
    17581 ggttcagggc gatattccgc aatcgctgaa atgggacgaa ggccagcttc ttaatacgct
    17641 gaagatttac tacaacgcaa cggcaccgct gatgggcaaa tcatcgttga ttatctggcc
    17701 ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag cactggacgg
    17761 tgagttgcgt gataaaggta gctcgctggt gaccgggatt gttgacgcgc gtcttaataa
    17821 gcagaaccgt tacgatacct acaacaccat catcacactg ggtaaaggtg caccgtacag
    17881 ctacgaatca gccgatcgct ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc
    17941 gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt cgtcgttcag
    18001 ccgtgggcca tatatccagc cgccgctgtc ggcaaatggt attgagctta ctgcggctat
    18061 ttgctacgag atcattctcg gcgagcaagt gcgcgataac ttccgcccgg ataccgacta
    18121 tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat ggcagcactt
    18181 ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa
    18241 cggcattacg gcggtgattg gcccgcaggg tgagattcag gcgatgatcc cgcagttcac
    18301 ccgcgaggtg ttaaccacta acgtgacgcc gaccaccgga ctcacaccat acgcacgtac
    18361 cggcaactgg ccgctgtggg tgctgacggc actgtttggt tttgctgctg tgttgatgag
    18421 tctgcgtcag cgacgtaaat aatcccttct tatcgtgccg gaatacatcc catatttcgg
    18481 cacgctcacc ggatgcggca tcagtgcatt atccggataa caatattctc ctatccaact
    18541 ttggcacatc tattgctttg ttatacaagg caaagcttta accagcatca gtacaacccg
    18601 gttgcaccac agcaatccac cggtagcacc aaaagagtgc attttaagaa ttttgcttcg
    18661 gaatggtgcg gcgcacctcc caaatataaa caataaaaca gcaatatctt tacatttagc
    18721 caaactaaat gttacctaac aggcacaaca ctacacaata aagttgcaga tgataacaac
    18781 acaaacactc acaacgggta tccatgcgtt cttaacgcag aagataaagg agttggatat
    18841 gcaattacgt aaacctgcca cagcaatcct ggccctggcg ctttccgcag gactggcaca
    18901 ggcagatgac gccgccccgg cagcgggcag tacgctggac aaaatcgcca aaaacggcgt
    18961 gattgtcgtc ggtcaccgtg aatcttcagt acctttctct tattacgaca atcagcaaaa
    19021 agtggtgggt tattcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact
    19081 caacaaaccg gacttgcagg tgaaactgat tccgattacc tcacaaaacc gtattccgct
    19141 gctgcaaaac ggcacttttg attttgaatg tggttctacc accaacaacg tcgaacgcca
    19201 aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa
    19261 gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg
    19321 cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat
    19381 catcagcgcc aaagatcacg gtgactcttt ccgtaccctg gaaagcggtc gtgccgttgc
    19441 ctttatgatg gatgatgctc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa
    19501 ctgggatatc gtcggcaagc cgcagtctca ggaagcctat ggttgcatgt tgcgtaaaga
    19561 cgatccgcag ttcaaaaagc tgatggatga cactatcgct caggtgcaga cctccggtga
    19621 agcggaaaaa tggtttgata aatggttcaa aaatcccatt ccgccgaaaa acctgaacat
    19681 gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg acaaagcact
    19741 gaactaatta caagaaccag gggcggaaat tctagccctc tcgattgtta cgtagcacgg
    19801 acagactata cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat acgctgggta
    19861 acaatcttcg agggtagcag ttaacgctgc taccctcttt tttctggagt agatttatgt
    19921 ctatagactg gaactggggt atttttttac aacaagcccc gttcggcaac accacctatc
    19981 ttggttggat ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc gcctggatta
    20041 tcgctttcct cgtcggttca tttttcggta ttttacgtac cgttccaaac cgttttctct
    20101 ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct
    20161 ttacctggta tctggtgatc ccggagctgc tgccggagaa aatcggcatg tggtttaagg
    20221 ccgagctgga tcccaatatt cagtttttcc tttcatccat gctctgcctg gggctgttta
    20281 ccgccgcccg tgtttgcgaa caggtacgcg ccgcgattca gtcgctgccg cgtgggcaga
    20341 agaatgccgc gctggctatg ggtctgacgc tgccgcaggc ttaccgctac gtactgctgc
    20401 ctaatgctta tcgcgttatc gtcccgccga tgacctcaga gatgatgaac ctggtgaaaa
    20461 actccgccat cgcctcgacc atcggtctgg tagatatggc agcgcaggcg agtaaattgc
    20521 tcgattactc ggctcacgcc tgggagtcgt ttaccgccat tacgctagcg tatgtgttta
    20581 ttaacgcttt tatcatgctg gtgatgacgc tggttgaacg taaagttcgc ctgcctggca
    20641 acatgggggg caaataatgt acgagtttga ctggagttcc attgtccctt ccctgccata
    20701 tctgctcgac gggctggtga tcacgctgaa aatcaccgtc acggcagtcg tgattggtat
    20761 tttgtggggc accatgctgg cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt
    20821 tgccaaagcc tacgttaacg tattccgctc gattccttta gttatggttt tgctgtggtt
    20881 ttacctgatc gtgccgggtt ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat
    20941 ccgtttaatt tcggcgatgg tggcgttttc gatgtttgaa gcggcctact attcagagat
    21001 tatccgtgcc ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg
    21061 aatgactcac tggcagtcga tgaaactgat tattctgccg caggcgttcc gcgcgatggt
    21121 gccgctgctg ctcactcagg gcatcgtact gttccaggat acctcgctgg tgtatgtgtt
    21181 atctctggcc gatttcttcc gtaccgcctc aaccattggt gagcgtgatg gcacgcaggt
    21241 tgagatgatc ctgtttgccg gatttgttta tttcgttatt agccttagcg cgtcgttgtt
    21301 ggtcagctac ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg
    21361 tatggtcact ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg
    21421 gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc
    21481 gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg tggttaacga caagaaaacc
    21541 gatctggcaa agctgcgttc tcgcgtcggg atggtattcc aacatttcga gctgttccct
    21601 catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa
    21661 gcaccggctc gtgaaaaagc attgaaattg ctggagcgcg tgggactttc cgcacatgcc
    21721 aataaatttc cggcgcagct ttccggcggc cagcagcagc gtgtggcaat tgctcgcgca
    21781 ttgtgtatgg atcctattgc gatgctgttc gacgaaccca catcggcact agacccggag
    21841 atgatcaacg aagtactgga cgtgatggtg gaactggcga acgaggggat gaccatgatg
    21901 gtggtgaccc acgaaatggg ctttgcccgt aaagtggcga atcgggtgat ctttatggat
    21961 gagggtaaaa ttgttgaaga ctcgccgaaa gatgcgttct tcgatgatcc gaaatcggac
    22021 cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac tctggcgcgc atacccaagt
    22081 gcgcgccatc tcacgcttta tcgtctcgcc cctcgtatct gttacatcct ggcgttaccc
    22141 ttgtcgcaaa gaagcacaca acaaggagca acaatggcac tgccaattct gttagattgc
    22201 gacccaggtc atgatgacgc tatcgcaata gttctcgccc tcgcctcacc agagcttgat
    22261 gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag aaaaaacctt acgcaatgtt
    22321 ctgcgtatgc tgaccttgct taatcgcacc gatattccgg tggcaggcgg cgcggtaaaa
    22381 ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg tctcgacggc
    22441 ccggcattac cggaaccgac gttcgcaccg caaaactgta cggcggtaga gctgatggcg
    22501 aaaacgctgc gtgaaagtgc ggaacctgtc acgattgtgt ctaccggacc gcaaactaac
    22561 gttgccttgc tgctcaatag ccatccggaa ctgcatagca aaattgcccg tatcgtgatt
    22621 atgggtggcg ctatgggact gggtaactgg acgcctgcgg ctgaatttaa tatttacgtt
    22681 gacccggaag cggcagaaat tgtcttccag tcagggatcc cggtggtgat ggccggtctg
    22741 gatgttactc acaaagcaca aatccacgtt gaagacactg agcgtttccg cgcgattggc
    22801 aaccctgttt caaccattgt tgccgaactg ctggatttct tcctcgaata tcataaagac
    22861 gaaaaatggg gctttgtcgg cgcaccgcta catgacccat gcaccatcgc ctggctgtta
    22921 aaaccggagt tgtttaccac tgttgagcgc tgggttggcg tggaaacaca ggggaaatat
    22981 acccagggta tgacggttgt tgattattat tatctgactg gcaataaacc gaatgccact
    23041 gtaatggtcg atgttgatcg tcagggcttt gttgatttac tggctgatcg tctgaaattt
    23101 tacgcttaac acacgtcatt gattaagatc accacgcgtt acctgggtgg tcttaatctg
    23161 tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc gccattggta
    23221 tcgatctcgg tactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa
    23281 ttccaaataa gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc
    23341 atattttagt cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag
    23401 cgttatttaa acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta
    23461 acgcgccaga actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc
    23521 tgcaacgtcc gattaaagat gtggtgatct ctgttccggc ttatttcagc gatgaacaac
    23581 gcaagcatac ccgtttagca gcggagttag ccggtttaaa tgcggtacgc ttaattaatg
    23641 aacccacagc agctgcgatg gcgtatggcc tgcataccca acaaaatacc cgttcgctgg
    23701 tgtttgatct cggtggcggc acgtttgacg tcacggtgct tgagtacgcc acgccggtga
    23761 ttgaagttca cgcctccgct ggcgacaact tcctcggtgg cgaagatttt acccatatgc
    23821 tggtcgatga ggttttaaaa cgcgcggatg tcgccaggac cacgcttaac gagagtgaac
    23881 tggcagcctt gtatgcctgt gtagaagcgg caaaatgtag caatcaatcg ccaatacaca
    23941 ttcgctggca gtaccaggat gaaacgcggg aatgcgaatt ttacgagaac gaactggaag
    24001 atttgtggtt gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg
    24061 cgcgtctgaa gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc
    24121 cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgatc
    24181 cgagcaccat tgtcgcgctg ggcgcagcaa tccaggcagc ctgccgctta cgcagtgaag
    24241 atattgaaga agtgatcctc actgatattt gcccttactc gttgggcgtt gaggttaacc
    24301 gccagggcgt ttccggcatt ttctcgccga ttattgaacg aaacaccact gtgcccgtgt
    24361 cgcgtgtaga aacttattca accatgcacc cggaacagga ttctattacg gttaacgtct
    24421 atcagggaga aaaccacaaa gttaaaaaca acattctggt ggaatccttc gatgtgccgt
    24481 tgaagagaac cggggcttat cagtcgattg atattcgctt tagttatgat atcaacggtt
    24541 tgcttgaagt tgacgtgctt ctggaagacg gcagcgttaa gtccagagtg attaaccaca
    24601 gcccggtaac attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga
    24661 aaatttatcc gcgcgatatg ctcatcaacc gcacctttaa ggccaaactg gaagagttgt
    24721 gggcgcgggc gctgggtgac gagcgagaag agattggccg ggtgatcacc gattttgatg
    24781 cggcgctgca gtcaaacgat atggcccgcg tcgatgaggt caggcggcag gcgagcgttt
    24841 atttagccat tgagatccca taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg
    24901 catcggcgca aaaaacacat cgtcaactaa acaacatcgc gattttgccg agagcataac
    24961 ctatcaccag caccgttaag ctgattttga taatgctggt aatgctctgt ggtttttcat
    25021 cctcatcatt tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg
    25081 gctcgagttt tactttcgtc aacagcatcc gggtgataat ggcctctaat ggatgagcat
    25141 ctccgccttc cagccttgta ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa
    25201 gtactacctg ttccgctacc cgatacaaat ccagtaacgg tgaatcttct tctgtctcgc
    25261 taccgactct cgatttccac cacgcccatg ctttgcgttt gaagaatagc gactcaataa
    25321 acgattgacc taacggcgat agttttccgc tatgtaaaat ttccgcccat aacagacgcg
    25381 tccgggcgct ctcaccagga acatatttca acggtttacc ttgcgcatct accatgctcc
    25441 gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgttgt gcgctccact
    25501 gcaacaacaa attatcggct tgtgtagatg ggtaacttcc ccagtacgcg cagagatcgg
    25561 taagcaggct ttccccctcg ccgcagtaca aacgttgtgc acactggtaa taccgggcat
    25621 cttcgctttc tggttcttcc atttgccact gttcggctac aggaatgagt tccgccatac
    25681 ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa
    25741 tgaaccacgg accttccatc gccaaaaacg cagtgacata gtcaggatgg taacggcagc
    25801 aacgttccag cgcgaagtaa tactcaacag tctggttctg caacgcaacg ggatatacca
    25861 gaaggccaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt
    25921 cttactcatc tattccttcg ctgtcattaa acgaatgcca gttcaaacgc tctgccagca
    25981 aagaagcaca atttaatgaa agatatcgcg cttctatagc gattgcacac agcggccagc
    26041 gtaattgatc gacatcctcc atgttccagg tatttaattg ctgaataaat ttctgccagg
    26101 cggagggctg aaaacgatca ctttctgaat ccagtaatgc cctgaataca cgtagtattt
    26161 catgctcagt ggcatcatct ttttcttcat catctgcttc ctcgacagga tttaccgcca
    26221 gtcgcagggc ctcttcatag gcctggcgta atgctttaaa cccttgcgga tcggtttcgg
    26281 gatgacataa cggtaagcga gcaagataag cctggcggat aatgtctatt tgcgtcgtgc
    26341 tttcaatttc gaggatttgc caacatgttt tcataggtcg ctcacatcct taagcgttac
    26401 cgggcattca ggctggacag ttaatggctg ccagggaagc gtggcataga aacgatcgct
    26461 tcccgcagcc agctcttcga acatccgact cccggcgcta tcgtatttgt acagggttag
    26521 cgcctcgcgt agcgaagcgt tgtcttcgca ttgagaagat tgccagtaga gacgccctag
    26581 tgaataggcg gaaaaatagt gcatccagct gtgatagtaa tggtgagctc gttcatagac
    26641 gcgcgattgc agccataaac tctcgtcttc cgtcagccac ttgttgagca caccgacccg
    26701 tgacagaaac cccattcgaa cataatccca agctttgatt cccccttccc cacaacacat
    26761 agcggtttgg gcaacgaatt gggtataaat caaccctctc tccgagccac cagcaattaa
    26821 tgctttccac tcttcagggg aagagcgatg ccatgtcaga tataagccag ctaaatcatt
    26881 ggcatgacca tcatcagtca tctgataaat agtctgaatt aaatcatcac gattatttat
    26941 tccccaggat gatgagagat cgaattcttt tatcccggta tacatttttg gcgagtcatg
    27001 cgcgggtttg caaccctgat taagtatttc catgggcgca gaaagcgcaa aaagaagata
    27061 ttgttcttcc ttattcattt gttaataatc ccttaagtgc attaactg
//
LOCUS       DAZFTN010000065        25951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFTN010000065 DAZFTN010000000
VERSION     DAZFTN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..478)
                     /locus_tag="ACHKV2_002998"
     CDS             complement(<1..478)
                     /locus_tag="ACHKV2_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940843.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYGKSLFI
                     SGNENLVNISAEKIDEIKNSKNFFSNIVNIIILLIKHPDKILPILHGCPVESIEVIKN
                     AKRTGQWSYEMTDKNGQSIEVSYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKI"
     gene            complement(666..2483)
                     /gene="malZ"
                     /locus_tag="ACHKV2_002999"
     CDS             complement(666..2483)
                     /gene="malZ"
                     /locus_tag="ACHKV2_002999"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO6940844.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2639..4012)
                     /gene="proY"
                     /locus_tag="ACHKV2_003000"
     CDS             complement(2639..4012)
                     /gene="proY"
                     /locus_tag="ACHKV2_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO6940845.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(4088..5407)
                     /gene="brnQ"
                     /locus_tag="ACHKV2_003001"
     CDS             complement(4088..5407)
                     /gene="brnQ"
                     /locus_tag="ACHKV2_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO6940846.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(5814..7109)
                     /gene="phoR"
                     /locus_tag="ACHKV2_003002"
     CDS             complement(5814..7109)
                     /gene="phoR"
                     /locus_tag="ACHKV2_003002"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO6940847.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(7167..7856)
                     /gene="phoB"
                     /locus_tag="ACHKV2_003003"
     CDS             complement(7167..7856)
                     /gene="phoB"
                     /locus_tag="ACHKV2_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO6940848.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            8046..9248
                     /gene="sbcD"
                     /locus_tag="ACHKV2_003004"
     CDS             8046..9248
                     /gene="sbcD"
                     /locus_tag="ACHKV2_003004"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO6940849.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            9245..12388
                     /gene="sbcC"
                     /locus_tag="ACHKV2_003005"
     CDS             9245..12388
                     /gene="sbcC"
                     /locus_tag="ACHKV2_003005"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO6940850.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            12514..13698
                     /gene="araJ"
                     /locus_tag="ACHKV2_003006"
     CDS             12514..13698
                     /gene="araJ"
                     /locus_tag="ACHKV2_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO6940851.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(13841..14749)
                     /gene="mak"
                     /locus_tag="ACHKV2_003007"
     CDS             complement(13841..14749)
                     /gene="mak"
                     /locus_tag="ACHKV2_003007"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO6940852.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            14874..15785
                     /gene="rdgC"
                     /locus_tag="ACHKV2_003008"
     CDS             14874..15785
                     /gene="rdgC"
                     /locus_tag="ACHKV2_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO6940853.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(15870..16319)
                     /locus_tag="ACHKV2_003009"
     CDS             complement(15870..16319)
                     /locus_tag="ACHKV2_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940854.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(16316..18595)
                     /locus_tag="ACHKV2_003010"
     CDS             complement(16316..18595)
                     /locus_tag="ACHKV2_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO6940855.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(18739..19023)
                     /gene="ppnP"
                     /locus_tag="ACHKV2_003011"
     CDS             complement(18739..19023)
                     /gene="ppnP"
                     /locus_tag="ACHKV2_003011"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO6940856.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(19095..19772)
                     /gene="aroM"
                     /locus_tag="ACHKV2_003012"
     CDS             complement(19095..19772)
                     /gene="aroM"
                     /locus_tag="ACHKV2_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO6940857.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(20030..20221)
                     /gene="yaiA"
                     /locus_tag="ACHKV2_003013"
     CDS             complement(20030..20221)
                     /gene="yaiA"
                     /locus_tag="ACHKV2_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO6940858.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(20271..20795)
                     /gene="aroL"
                     /locus_tag="ACHKV2_003014"
     CDS             complement(20271..20795)
                     /gene="aroL"
                     /locus_tag="ACHKV2_003014"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO6940859.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(20978..21436)
                     /gene="yaiI"
                     /locus_tag="ACHKV2_003015"
     CDS             complement(20978..21436)
                     /gene="yaiI"
                     /locus_tag="ACHKV2_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO6940860.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            21556..22365
                     /gene="proC"
                     /locus_tag="ACHKV2_003016"
     CDS             21556..22365
                     /gene="proC"
                     /locus_tag="ACHKV2_003016"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO6940861.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(22382..23497)
                     /gene="adrA"
                     /locus_tag="ACHKV2_003017"
                     /gene_synonym="dgcC"
     CDS             complement(22382..23497)
                     /gene="adrA"
                     /locus_tag="ACHKV2_003017"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO6940862.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(23599..23919)
                     /gene="psiF"
                     /locus_tag="ACHKV2_003018"
     CDS             complement(23599..23919)
                     /gene="psiF"
                     /locus_tag="ACHKV2_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO6940863.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(24038..25453)
                     /gene="phoA"
                     /locus_tag="ACHKV2_003019"
     CDS             complement(24038..25453)
                     /gene="phoA"
                     /locus_tag="ACHKV2_003019"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO6940864.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(25554..25814)
                     /gene="iraP"
                     /locus_tag="ACHKV2_003020"
     CDS             complement(25554..25814)
                     /gene="iraP"
                     /locus_tag="ACHKV2_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO6940865.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         6552 a         6837 c         6295 g         6267 t
ORIGIN      
        1 ctattttata ttcatgtgta ctagaatcat tttggttgca ggagttacag atttttaagc
       61 ataattttcc atcagattta tcatctatgg tatcagtgaa aatataggac acttcaatac
      121 tctgcccgtt cttatcagtc atctcatatg accattgtcc cgttcgtttc gcattcttaa
      181 tgacttcgat cgactctaca ggacaaccat gaagaatggg cagaatttta tcaggatgct
      241 taatgagtaa aattattata tttacaatgt ttgaaaagaa atttttgcta ttttttattt
      301 catcgatttt ttccgctgaa atgtttacta agttttcatt tccggaaatg aaaagcgatt
      361 tgccataggt gcttttttta aattgttctt ctgttttaga aaagacatcc tctttaaaag
      421 gtggcaactc agtaccagta ataaacggtt tcaataaatt cgaatttgaa attgtcatat
      481 ctttgctcca ctacagggtt atcctggagc atgttgtaca taacatcatg ggcaaacaat
      541 accaaaaatc gctcatatcg atcgggacaa atatttaaca aattcagccc tgacattgag
      601 cagctttttt gcgctgcgta gtgtcagggc tggtgactaa cgacgtaaca aataaaacag
      661 atgctttagt tcatccacac cgtagccgaa atggcaggca aagccagaat cccgtctgtc
      721 agttgcccat ggccttcttt gcgttgccat tgcacgacat taagaaacgg tgacgcgggt
      781 agcaccactt cacaggcttc gccacggtta attgccacca gtacacgctg ctgattcagc
      841 acgcggacaa ataccaccac gttatcttcc gcatacagca cctgacagcc gccacgacgc
      901 agcgcctgac ttttcttacg cagcgcaatc attcgctggt acaacgcgaa taacgccata
      961 tcctgttttt ccacctgcca ggggaacggc ttacggcaaa acggatcatt tttgccatcc
     1021 agtcccactt catcaccgta ataaatgcac ggtacaccag gccaggtgaa cagccagacc
     1081 actgccaggg gcaggcgcgc aacctcccga ccgagcagcg ttttaaatcg cgcagtatcg
     1141 tggctgtcga gctgattaaa catacgtaat tgctgttgat gagaaagccc tgcgcggtaa
     1201 ttatccatcc aggccataca ggtttgagcg tcaatttgct gcggatcgta agagatgtcg
     1261 gtattggcaa gaaatcccca caacgggaat gtgaagccgc gatagttcat ggcggcatct
     1321 tccacatcgg cctgtaacca ttgccgtgca tcgccaaaat gctctccaac tatatacgct
     1381 tccggctggg tttctttcgc cgcttcggtg atcccagcaa cgtgctgcaa attattgcgc
     1441 gccccacccg cttcccccag catatgcacc acatccagcc gccagccgtc catattccac
     1501 ggcgctttca gccagtggcg gacaatactg tcttccccgc gataaatttc attcaccaga
     1561 ctttccgact gataatccag cttcggcaag ctggcatagc caagccagtc gagcgccgtg
     1621 ccatcatcac taaacgagta ccagtcgcgc cagggcgatt cggggttgtg acaagctccc
     1681 cccgtgccac gattgtgcct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg
     1741 ccatccagca ccagccgcat tcccagctgc tgcgtattgt ggcgtaagcg cagcaacgcc
     1801 ccatcaccgc caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgtacg
     1861 ctgggagctt taaacaccgg attgagatac agcgccgtca cgccaagctt tttcagatac
     1921 ggcagttttt cgcttatccc gtccagatcg ccgccataga acgttgatcc gcccgcctgc
     1981 gccgtgaccg gttcatccca gtcacgcaag atgatctctt gtccggctgc atgatggtaa
     2041 tagacatgat cctgttcagc ttcacgagga agactacgcg caaaacgatc agggaagatc
     2101 tgataaaaaa tctgatccgc agcccattgt gggccgatat ccggtacatc gacggcaaac
     2161 tgctccagtc gtgccggcgg cattcggctg aagccctgcg gtgtaaacca acgctggcga
     2221 tcgtgccaca gcagtttgaa actgtaacgc cgccggggtt gtccgctgga gagatcaatc
     2281 gccgcacgcc atgcggtgac gcctggctgc ggctgactgc gctgcttatg cattggtact
     2341 gacatttctt cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc
     2401 gtcagccaca gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc
     2461 aggtgccatg catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata
     2521 gcctaatgca ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat
     2581 aactttcgga atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaagtatt
     2641 attggttttc agccagctga cgatcgtggc gacgcttaaa catccagcca atcaacagca
     2701 caacaatcca cgcgaaaccg acatacagcg agatacgcgt atccgggtga taaccaatca
     2761 acccgataat aaagagcagg aaaatcagcc cgccgatggt cgttgctacc ccacccggca
     2821 ctttaaattt cagcgcctta acttcttctg gcggcaaacg gcgacggaag gcaatttgcg
     2881 acagcaggat cataatccac acccacaccg tggcgaaggt tgccagcgaa gcgatcacca
     2941 ggaagacgtt ttccggcatg atgtagttca gatacaccgc aaacagcagc gcggtagtca
     3001 tcaccagcac cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa attttcggcg
     3061 cgctgccctg ctctgccata ccgtggagca tacggcctac gccaaataca tcactgttaa
     3121 ttgccgacag cgaagcagtc agcacaacaa agttaagaat gctggcggca aaggtaatgc
     3181 ccatatgctg gaacgtcagc acgaacgggc taccggcagt gccaacctga ttccacgggt
     3241 agatagacat aatgacgaac agcgtaccga cgtagaacac cagaatacgc atcggcacgg
     3301 agttaatcgc acgcggtatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga
     3361 taatttcgat cccaccgtaa gcaaacatca ccatttgcaa cgacattacc atgccaagcc
     3421 agccgttact gaagaagccg ccgttgctcc acaggttatg aataccggtc ggttgcccgc
     3481 cgttgccaat cccccagatg atgatgccga aaccggcgac aatcatgatg atgatggtgg
     3541 cgactttaaa gaacgagaac cagaattcca gctcaccgaa taccttcacg ctcatcaggt
     3601 ttacggcgca aatgatcagc accacgctca gtacccaaat ccagtgcggc accgtcggga
     3661 accagacacc catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt
     3721 caaagcagta ggtccagccg gtaatgtaac ctgccagcgg gccgaggttt tcctgcgcat
     3781 aacgcgagaa agagctggcg gccgggttat gtaccgacat ttcccccagc gcacgcataa
     3841 tgatatacgc cgcgatacca ccgataatat aggccaacaa cacgctcgga ccggccattt
     3901 tgatggcgtc tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc agtgccataa
     3961 agcgtatgtg gcgggtactt agcccacgct ttagcttgtt cttactttcc atcaattcct
     4021 aacccatcaa cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt
     4081 cagtgattta gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg
     4141 ccagaaccac catcaccact gttggcatta accacgccag accttgttcg gccagcggta
     4201 aacgctgcgc ccaggacggt aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa
     4261 taccaaaaag caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc
     4321 atgagcgtgt aaaacttaat acaaccagtg cgatacacgg cggataaata gcggtcagca
     4381 ccggtacgga gatctggatc agctggctta agccgaggtt agaaaccacc atcgagaagc
     4441 cgccgaggat aaacaccagc gtacgataag agagcggtac gtactgggca aagaattctg
     4501 cacaagcaca ggtcaggcca actgccgtta ccaggcaggc gatgaagatt aacgccgcca
     4561 gcaggaagct accgccgccg ccaaaggtgt gctgaacgta agcatgcaga atagcagcgc
     4621 cgtttgcaga ctgatcgacc agcgacgcgc tgtctgaacc cagacggaac agcgccaggt
     4681 acagcagagt cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagta
     4741 ggcgcgcttc ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca
     4801 ttgcgcccag cgtatccatg gtcagatagc cgttgacgaa gccgttagaa aacgcagcgt
     4861 tttgataagc ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc gcggcaacag
     4921 acaggatgac cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca
     4981 gcttgcccgg atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtagataa
     5041 acagcggcag cgcggaatca cccgtcagcg gcgcaatccc cacttcaaaa gaaacggtag
     5101 ctgtacgcgg cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt gccagcagta
     5161 cgccagcgac tttaccgatt ggcgtgctga ggctgtcaac accgccgcca acttttgcca
     5221 gcgccactac cgttaatacc ggcaggccaa cggcagtaat gaggaagccg aatgccgcag
     5281 tccagacgtg ttcgcctgcc tgtaagccga ccattggagg gaaaataatg ttacctgcgc
     5341 cgacgaacaa cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat
     5401 gggtcataaa tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc
     5461 cctggggatg atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc
     5521 gcggcgaaga atagtttttc gatttaccgc gttaatgcag tcagtatgac agcacctgaa
     5581 gcgcaattta aacgcttata acgttttaaa gcaaggaggg atctaaaaac cagaggtata
     5641 tagcgataag aaatcaccaa ccagtataaa acactatttc cgtgaaaaag acatggctaa
     5701 tcatgcgaac aaaaaatcac caaccgttgt aaaatctaac gcccgtattt tgactgcaaa
     5761 gaaaataagc cagcatcggc tggcttatgg caataaaaga tgacaaaggc ggattaatcg
     5821 ctgtttttga caattaaacg ttccgggata acaaaactga aacgtgttcc ttttcctact
     5881 gtactctcaa tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac
     5941 cctaatccgc taccgccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg
     6001 gtcaggcgcg gaatatgctc cggtgcaatg cccggtccgt tatcttcaac gctaaattcg
     6061 gcaccgtgcg gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc
     6121 acggcgttat agaccaggtt cgaaatcaca ctgcgtagct gatcttcgtt gccagacacc
     6181 ttgaggccgt tatctatctc gaaggtaaat gtctgttttt tctgactcag agtctgagcc
     6241 tcgcgctcaa caacgcgcag catcatcggc acatcaacct tttcattgag caattgcgtc
     6301 ggtgcggctt caattttcga cagcgttagc aactgcttca ccagcccttc catccgctgg
     6361 gtctgctcgc gcatggtgtg caacgctttt tcgcgtaccg cgccttccag cggctgctca
     6421 tccatcatct ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg
     6481 ttggcaaaaa agttacgccg cgccccttcc agttgatgca tttgcgtgac atcacgcgcc
     6541 accatcagca actgtttgtg ggtataaggc atgacgcgaa tttccagatg ccgcccggtg
     6601 ttgagcacca gattgagcgg gcgagaaaaa tcacgcgttt tcaaatattg cgtaaactcc
     6661 gggtaacgca gcaggttaag gatgttctgc ccgttatctt ccggccagcg caaaccaaga
     6721 atctgttgcg ccagaccgtt acaccagaaa ataccgccct cttccgtggt cagcaccacc
     6781 gcgtcgggca gcgactccgc gccgctacga aagcgtttaa tcagattgcc cagttcacgg
     6841 cggcgttttt tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta
     6901 ccacgccccg gtggcggggt catactgcga tccacccaca gccaccatga aaggcgcaat
     6961 aaattccaga aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg
     7021 taaccaaaaa atgcacccag gatgaaagcc gggaggcagc aaagtagcag ctccagcacc
     7081 agccttttcc acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc
     7141 ctgctctgcg tccgatgagc aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg
     7201 cgcacggtct gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga
     7261 atgtggacat cgaccgtgcg gtcttccaca tacacgttag ttccccagac gtggtttaac
     7321 agctgctcgc ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat
     7381 tctgtcggcc ccatctccag cggctcttcg cccgccatca ctcggtgaga tgtcgggtcg
     7441 agacttaatc cctgcatctc aatcacctct tccaccgcca ttggcgaaat acggcgcatt
     7501 accgctttga ttcgcgccac cagctccttc ggcgaaaacg gcttggtgat atagtcatcc
     7561 gcgccggttt caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc
     7621 actggaatat cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag
     7681 ccgccaggta acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc
     7741 acagcactgt cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag
     7801 cagaccattt cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt
     7861 tgccctgttg taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta
     7921 tgacagattt atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcgcag
     7981 aaaaaagcaa atggcacatc tgtttgggta taatcgcgcc catgcttttt cgccagggaa
     8041 ccgttatgcg catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa
     8101 gccgcgaagc tgaacatcag gcttttcttg actggctgct ggagacagca caaacccatc
     8161 aggtggatgc gattattgtt gccggtgatg ttttcgatac cggctcgccg cccagttacg
     8221 cccgcacgtt atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg
     8281 tactggcagg aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt
     8341 tcctcaatac taccgtggtc gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg
     8401 acgggacgcc aggcgcagtg ctgtgcccca ttccgttttt acgtccgcgt gacattatta
     8461 ccagccaggc ggggcttaac ggtattgaaa aacagcagca tttactggca gcgattaccg
     8521 attattacca acaacactat gccgatgcct gcaaactgcg cggcgatcag cctctgccca
     8581 tcatcgccac gggacattta acgaccgtgg gggccagtaa aagtgacgcc gtgcgtgaca
     8641 tttatattgg cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg
     8701 cgctcgggca tattcaccgc gcacagatta ttggcggcat ggaacatgtt cgctattgcg
     8761 gctcccccat tccactgagt tttgatgaat gcggtaagag taaatatgtc catctggtga
     8821 cattttcaaa cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta acgcaaccca
     8881 tggcagtgct gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg
     8941 tatcgcagga gccacctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg
     9001 atattcagcg caaaatccag gcattaaccg aatcattgcc tgttgaagta ttgctggtac
     9061 gtcggagtcg tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg
     9121 aactcagcgt cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg gatgaatcgc
     9181 agcagcaacg tctgcagcat cttttcacca cgacgttgca taccctcgcc ggagaacacg
     9241 aagcatgaaa attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa
     9301 gattgatttc acccgcgagc cgttcgccag caacgggctg tttgccatta ccggcccgac
     9361 cggtgcgggg aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc
     9421 gcgtctctct aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgctgaatg
     9481 tctggcggag gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa
     9541 tcgggcgcgt aaccaacccg acggtaattt gcaggtgcca cgcgtagagc tggcgcgctg
     9601 cgccgacggc aaaattctcg ccgacaaagt gaaagataag ctggaactga cagcgacgtt
     9661 aaccgggctg gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc
     9721 tgccttcctg aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt taaccggcac
     9781 tgaaatctac gggcaaatct cggcgatggt ttttgagcag cacaaatcgg cccgcacaga
     9841 gctggagaag ctgcaagcgc aggccagcgg cgtcgcgttg ctcacgccgg aacaagtgca
     9901 atcgctgaca gcgagtttgc aggtacttac tgacgaagaa aaacagttac ttaccgcgca
     9961 gcagcaagaa caacaatcac taaactggtt aacgcgtctg gacgaattgc agcaagaagc
    10021 cagccgccgt cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca
    10081 actggcggcg ctcagtctgg cacaaccggc acgaaatctt cgtccgcact gggaacgcat
    10141 cgcagaacac agcgcggcgc tggcgcatac tcgccagcag attgaagaag taaatactcg
    10201 cttacagaac acaatggcgc ttcgcgcgag cattcgccac cacgcggcga agcagtcagc
    10261 agaattacag cagcagcaac aaagcctgaa tacctggtta caggaacacg accgcttccg
    10321 tcagtggaac aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg
    10381 cgagcatctg cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct
    10441 tgcggcgatc acgttgatgt taaccgccga tgaagttgct accgccctgg cgcaacatgc
    10501 tgagcaacgc ccactgcgtc agcgcctggt cgcgctgcat gggcagattg ttccccaaca
    10561 aaaacgtctg gcgcagttaa tggtcactat ccagaatgtc actctggaac agacgcaacg
    10621 taatgccgca cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc aacttgccga
    10681 tgtgaaaacc atttgcgagc aggaagcgcg catcaaaacg ctggaagctc aacgtgcaca
    10741 gttacaggcg ggtcagcctt gtccactttg tggttccacc agccatccgg cggtcgaggc
    10801 gtatcaggcg ctggagcctg gcgttaatca gtctcgatta ctggcgctgg aaaacgaagt
    10861 taaaaagctc ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat taacgaagca
    10921 gcttcagcgc gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca
    10981 acaatggcaa gccgtcacgg ccagcctcaa tatcaccttg cagccacagg acgatattca
    11041 accgtggctg gatgcacaag atgagcacga acgccagctg cggttactca gccaacgaca
    11101 tgaattacaa gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat
    11161 tgaacaacgc cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca
    11221 ggaagatgaa gaagagagct ggttggcgac acgtcagcaa gaagcgcaga gctggcagca
    11281 acgccagaac gaattaaccg cgctgcaaaa ccgtattcag cagctgacgc cgattctgga
    11341 aacgttgccg caaagtgatg atctcccgca cagcgaagaa actgtggcgc tggataactg
    11401 gcggcaggtt catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca
    11461 ggatgttctg gcggcgcaaa gtctgcaaaa agcccaggcg cagtttgaca ccgcgctaca
    11521 ggccagcgtc tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact
    11581 aacgcagctg gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct
    11641 ggtcactcag acagcagaaa cgctggcaca gcatcaacaa caccgacctg acgggttggc
    11701 tctcactgtg acggtggagc agattcagca agagttagcg caaactcacc aaaagttgcg
    11761 tgaaaacacc acgagtcaag gcgagattcg ccagcagctg aagcaggatg cagataaccg
    11821 tcagcaacaa caaaccttac tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg
    11881 gggatatctg aattcgctaa taggttccaa agagggcgat aaattccgca agtttgccca
    11941 ggggctgacg ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgg
    12001 gcgctatctg ttacagcgca aagccagcga ggcgctggaa gtcgaggttg ttgatacctg
    12061 gcaggcagat gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgttag
    12121 tctggcgctg gcgctggcgc tttcggatct ggtcagccat aaaacacgta ttgactcgct
    12181 gttccttgat gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc
    12241 gctggatgcc ctgaacgcca gtggcaaaac catcggtgtg attagccacg ttgaagcgat
    12301 gaaagagcgt attcctgtgc agatcaaagt gaagaagatc aacggcctgg gctacagcaa
    12361 actggaaagt acgtttgcag tgaaataact attcagcagg ataatgaata cagaggggcg
    12421 aattatctct tggccttgct ggtcgttatc ctgcaagcta tcactttatt ggctacggtg
    12481 attggtagcc gttctggtag ttgtgatggt ggtatgaaaa aagtcatttt atctttggct
    12541 ctgggcacgt ttggtttggg gatggccgaa tttggcatta tgggcgtgct cacggagctg
    12601 gcgcataacg taggaatttc gattcctgct gccgggcata tgatctcgta ttatgcgctg
    12661 ggggtggtgg tcggtgcgcc aataatcgca ctcttttcca gccgctactc actcaaacat
    12721 atcttgttgt ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg
    12781 tcgtacctga tgcttgccat tggtcgcctg gtttccggct ttccgcatgg cgcatttttt
    12841 ggcgtcggag cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg
    12901 gcggggatgg tttccgggat gacagtcgcc aatttgctgg gcattccgct gggaacgtat
    12961 ttaagtcagg aatttagctg gcgttacacc tttttattga tcgctgtttt taatattgcg
    13021 gtgatggcat cggtctattt ttgggtgccg gatattcgcg acgaggcgaa aggaaagctg
    13081 cgcgaacaat ttcacttttt gcgcagcccg gccccgtggt taattttcgc cgccaccatg
    13141 tttggcaacg caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt
    13201 tccggttttt cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg
    13261 ctgggaaata tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca
    13321 gtgactgact ttataattgt actggcactg ctgatgctct ttttctgcgg cggcatgaaa
    13381 acaacgtcgc ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct
    13441 ctgcaaatat tgttactgca aaatgccaaa ggcggagagt tattaggtgc cgcaggtggg
    13501 caaatagcgt ttaacctcgg tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg
    13561 ctggggctgg catataatta cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg
    13621 tcatcgttgc tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg
    13681 gcgaaaccac tggggtagat tactgtcacg gtgtgcgttg cttattgccg gatgcggcgt
    13741 gaacgcctta tccgccctac gcggttctgg cacattttgt aggcctgata agacgcggca
    13801 agcgtcgcat caggcatgat gcgccaattg cctgggtttt ttactcttgt ggccataacc
    13861 acgcagcgcc gcgtacgccg ctggaatcac cgtgcttcgc cttacgcacc ggcgtttcac
    13921 attcgccgcc gaagacaaat tgtttaatca actgcggaac cgtttgatat aaacggtcta
    13981 cattgctcat cccgcccccc aggacaatca catccggatc gagaatattc acgacatgtg
    14041 ccagcgattt tgccagccgc agctcgtagc gacgcaatgc cagttccgct accggatcgc
    14101 tttcttcaac caggcggata atttcactgc ctttcagcgc atgtccgctc aaacgacgat
    14161 aatccgtcgc gaatcccgtg cccgaaataa aggtttcaat acaaccttgt ttaccgcaat
    14221 aacaagggac ttcctcgcga taacgcagtt cgtcttcgtc catccacggt agcggattgt
    14281 gtccccactc acctgccgtg ccattgccgc cgatatgcgc ccgcccattg aatgccacgc
    14341 ccgcgccgca tcccgtgccg ataatcacgg caaataccgt ctgcgctccc gctgccgcgc
    14401 catctactgc ttctgaaacc gccagacagt tggcgtcatt tgccagccgc acttcccgct
    14461 gcaacctcgc gcttaagtct ttatcgaatg gctgaccgtt gagccaggtt gaattggcat
    14521 tcttcaccac accggtgtaa ggcgaaattg agccaggaat gcccatacct accgttccgc
    14581 gctgccccgt cgcctgctcc gccatatcaa ccaacgtggc gatcgtttca atagtctgcc
    14641 ggtaatcatc acgcggcgtg ggcagacgat ggcggtacaa ctgctcccct gcatcgccca
    14701 gtgcaatcac ttcagttttg gtgccgccta aatcgatacc tatacgcacg gtactctcct
    14761 tatttttttc aatatcaata gcgtagagac ggacaaccgg attggcaatg caaggccgcc
    14821 gacaattcgt tatcatgccc gctaaattta acgacaaggc cgtggaaatt atcatgctgt
    14881 ggttcaaaaa tttaatggtt taccgtctta gccgcgagat ttcgctgcgt gcagaagaga
    14941 tggaaaaaca gctagcctcg atggcattta ccccatgcgg cagccaggac atggcgaaga
    15001 tgggctgggt tcctccgatg ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc
    15061 aaattgttat ctgcgcgcgc aaagaagaaa aaatcctacc gtctccggtg attaaacagg
    15121 cgctggaagc gaaaatcgcc aaactggaag cggaacaggc gcgtaagctg aagaaaaccg
    15181 aaaaagattc gctgaaagac gaagtgctgc actctctgtt gccgcgtgct ttcagccgtt
    15241 ttagccagac aatgatgtgg atcgacacgg ttaacggttt gattatggtg gactgcgcca
    15301 gtgccaaaaa agcggaagat acgctggcac tgctgcgtaa aagcctgggg tcgttaccgg
    15361 ttgtaccatt gagcatggca aacccgattg aactgacgct gaccgaatgg gtacgttccg
    15421 gtagtgcggc acagggcttc cagctgcttg atgaagccga gctgaaatcg ttgctggaag
    15481 atggcggagt gatccgcgcg aagaaacaag atctgaccag cgaagagatc accaatcaca
    15541 ttgaagccgg aaaagtggtg actaaactgg cgctcgactg gcagcagcgc attcagtttg
    15601 tgatgtgcga cgatggttcg ctcaagcgtc tgaagttctg cgacgagctg cgcgatcaaa
    15661 acgaagatat cgaccgtgaa gatttcgccc agcgttttga tgccgatttc atcctgatga
    15721 ctggtgaact ggcagcgtta attcaaaacc tgattgaagg attaggtggc gaagcacaac
    15781 gttaattgct gattttcctt taatgccgga tgcgacgcct gccgcgtctt atccggcgta
    15841 cgaagccaca ccaggcatat aattattcgc tacggcgggc aataattttt tagcgcagca
    15901 atattatgcg ttttacgccg taactgatct aatcgcctca tccctattcg cctgtcagca
    15961 gcacgcagaa tattcactaa cccccattta tcgctggtaa attgttttgt taacaactcc
    16021 gcttttgggg tgtcaatata ttcacgcaat aaatcagaaa ttgaacaaat atccgtcagg
    16081 taaggatacc agcaagtttc accgtgaaaa ttacgcactc caccgttgcc tgcaataaag
    16141 tctttcgggt aatcccagat cacgttttta tccaacgtga tccaataacg cggtaaatcg
    16201 gtgcttccac cattttggct gcgcatggga taacgggtac agtgaatttg caggttgatc
    16261 gttggcgtca gtaagtcata gatctcccgc tgtaacttgc tccaggggcg ttgtgtcatt
    16321 gtttttcctc aagccggtga cgcgcttcat tgcgaatttt ccgcgttgcc ccggttttca
    16381 ctaaatcccg taactgcgaa agatcgggtt tatcgacaaa taaaagtaat gatggatcct
    16441 cttttagcca caccgttttc acatcggcag ccagctgtgg attattaata gccagcgacc
    16501 gatcctgaga ctcagtcaat gtcgttaaca acgacgccgg ggtatgtgcg ttgcgtaatg
    16561 ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc acgccaccat tcatcatctg
    16621 atttatcttt tatcgtcgcc agttccgcaa aaatatcgct atcctcatgg ttccagcggt
    16681 gtaatcctct ttcactttgt ctacgggtgc tatttttcat actgacaaca gccagcgccc
    16741 agtcagagtt gaagcgatac tgttgatcga gtttgttgat aattccggaa ggtaattctt
    16801 tattatcaaa taattggtta cgcattcgat agcggtattc agcgggtaaa tcaaccagta
    16861 aatcaatggc attatttaac atctcttcgt caacaacacc attatcaatc agcgccagct
    16921 gtacttcgta ggcttgctct tgatcttctt gccgtttgct catcattaaa tttaatgtgc
    16981 gttgccataa ctgtttctta tattccagcg gcaataattt atcgcgtgcc agctcatccc
    17041 agacggtggg aacatcatgc tgagtcaata gataactaat cacgtccttc gatgtcagat
    17101 aacgcgcacc ttcgcgcaaa ttgtgcacat cttcttttgc cagagacagt tgacgcatct
    17161 ccggcaacgc tatcaataac acctttacga tatttttatt ttctttatta tccagataca
    17221 tttgttctgc gatacgttca atatcctctg tacgcagggt tgctgacagt tgggtaattt
    17281 ttaattcagt aagcgtccgg gctaatgctt cacgaacttt cagtgattta tctttactca
    17341 acatcactgc ttgttgtgcg gttaaatctg ctccggtaat tctttcacgt aactccgggt
    17401 cttcgttatt tatcaactca tcggccactg cagtcggaac cagccaggcg taatcgtaat
    17461 ggctgagtaa tgtcttgcgt acactgagaa aggggctatg acttaacgac gcaacaatat
    17521 ctggagaaag attatcctgt ctggcctgct ccagctgtac gctctcgacg ggtacagaca
    17581 ttaatttttt attcacatcg gcggaaatat tactgttttt aaccagccct ctggcaacag
    17641 cctcatcttt aattaattca tcggtaagcc aatgcatctg ttgagcgggc caggactgcg
    17701 ccatcgattc acgcgtctgg tcatcggggt gctgtaacag ttgcagcata acggcgggtg
    17761 ataaactctc tgtatgagtc agaaaacgtg ccaccagtgg atcttgatgc tttattttat
    17821 tcgctgggaa attatcgagt ttccccgttt caatccattt taatttctca ttaatttcag
    17881 gagcggcttt atctcgaaaa agcggtaaac cctgaacgat gttgtctgcg acgctatcat
    17941 agtcaggata cgcattggaa atgttatcgg tttcgatttc agtgtgatca taaagatcgg
    18001 cagtcaccag tgaataggct ttttgaatat tctcatccca gttatatcct tcaccacttc
    18061 catcttcatt gatataaaat tcagctttaa tgacttcaaa ttgcgttgct ttatcggaaa
    18121 caataatgcc tcgcgcacta attcccttca tcacttcaaa gttatgatca ttgtaatcag
    18181 cataaactaa acctttcgct gtcatctgtc ctttgacata cgcgaaatca tgattaataa
    18241 agttatcgac aataacatta cctaatacaa tcagatgacc attacctgac agataatcat
    18301 cataatcgcc ggaaatcgtt aaattaccgt tcaccaccag tttattttta ttgctgaagt
    18361 tagcgggtaa tgataggtca ccattatgat aataccagcg gatattatct tttagcatat
    18421 aagcagcttc catactatcg tcggttgccg ctttaaccac gttgcgctca tctgccgtca
    18481 taagatgaaa gtcagaggtg ataaggtcct cgctactgat ccactctgtg gcacatgcat
    18541 tgccagtcat aaaaaataag accatacaga tgaccaatat attaattcta ttcataatct
    18601 tcaatgatat ttatatcaac cttgatgaat tatgaatata tcggaaattg aaaaaatcgc
    18661 agcaaaggaa atattattat ggggttatga gaggacattt cagaaaaatg ccctcccgtt
    18721 caagcggaag gcgaggaatt acagatagcg gcacagataa gaggtgggtt cggcaacttg
    18781 cagatgaaac tcactgtgac cgggaacatt aaacaccgaa ccggcttcat acacttgcca
    18841 gtcggtcgcg tcaggcagta acacattcag cgcgccactg attaccgtca tctcttccgg
    18901 ctcagcggtg ctgaaggtgt attcgccttc aaccataaca cccacgctgg cgcgaccagt
    18961 gctgctgctg gaaaagccga tcgatttcac tttgccggaa aagtactcat tactttgaag
    19021 cataaactgg ccctgtctgg tagttgaata tctacttcac tataggggcc agatgcgctg
    19081 gctgtcacgc aaaattacac cagtaattcc gcagccagcc gtgcaatcaa tacgttagag
    19141 agcaagacag gaacatcgag ctgtttttgc agtaaatcgc gatgacgctg gttaaatccc
    19201 aaacaatcca gcatgatgac atctgcgcct ttcgccagta attctttccc ggcatcaatg
    19261 attttttgtt ctgaatcatg aatggggtta cccaatgaaa atacaggcgg tttctgcaaa
    19321 atttgccatt tttgcgcctg aacggtcagc aactcctcaa ccggtacgat aacccccacc
    19381 tgatgatctt caacaataga ggaaaccagc ggtggcaata tccgcgacgg ctcaagaaag
    19441 atcgtattac gcgcagtcat actactaatg tttgccgtac tcattaatat aatgacgtcg
    19501 tagccctgat tatcgagcac ttcaaccaca ccttgcaggt cacgctctac tttgcgacgc
    19561 gaaacatggg ccagatggtt gtcatttaat aatgtgagaa ttgtgtcttc gcctgcttct
    19621 ggcgcgtact ccgccatcac ttcttcgcgg cttaacttcc ccagcaggct atgatgggaa
    19681 atattatctt cgtcaatgta ttctgtcagg agcggcaaaa cttcctgcat aggtacaatg
    19741 ccgatggtta gaatcgccaa cgacgcactc attgttcttc caccttactt ttatttcgtc
    19801 gttaattacc gggcaagtgt taagcaccac tgctgacact tacttgtgct acaaatgaca
    19861 aaaagcgtag cagcagacgt gcacggcata atgtaaagat tccaaatgat tccagtaatg
    19921 gattgttatt gtttaatatt ctaattattt gaaaaacatg aattatgaaa aatgtgacgc
    19981 agatcccttg tagtggcact cagaatccct tccgagcagt ctgatgcggt cactctttgt
    20041 cagggtcctc atagcgtttt ttcgcatcca tcgcttcctg agcggtcgga tgttcactga
    20101 tcaacgagtc tggtttagga tgatcggctc tgagttggta ccaggttacc gtctgtcctg
    20161 gctttccttt ttcaattgtc actatatatg cttcacgagg ataaggcggt ttcgttggca
    20221 tcgttcttcc ttatttcacg ggatgaacgt taagtatagg cgctcgaaaa tcaacaattg
    20281 atcgtctgcg ccagggcgct gcgaatttca gaaatcacct ggctgggttc gtttgttgcg
    20341 tcgatgataa tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag cacttcctga
    20401 acttcttcgc tcagcggttt tcccgttaag gttggccgta aatcttcttc cggtgcagct
    20461 tgcagtcggt taaccaggac tgatactggc gcacacaaat aaaccacgat cccgttattt
    20521 tgcatgaagt gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc gataacggtg
    20581 gatggcgcag ttaccgcttc cagcgccgcc gtttctctgg cgcgaaatcc cgcccactct
    20641 tccctttcga cgatctccgc gaccgtcata ttgagctgtg attgcaacca ctgatcggta
    20701 tcgacaaacc gacggttaag cgaatcggca agggccattc cgaccgttgt tttaccacag
    20761 ccccgaggcc cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca ataggtcgca
    20821 atgcggcgaa agccggtgtc atgagaatag cgatcatacc atcaaactag tacaatttcg
    20881 attgtaaaga aaaaattcca cttaaagtga aaatctcaat acatccctta ctataccaat
    20941 aaatattcaa gaatgaagtg taaataataa attacattta gccacgacta cgttgcactt
    21001 ccagccacca cttctccagc tccgcggcaa aggcctggcg gtcacgttgt gaaaggctat
    21061 ctggtccgcc ggtctggatc ccactggcac gtaaggtatc cataaaatcg cgcatcgtca
    21121 ggcgctcacg aatggtcgct ggcgtgtaac gttcaccgcg cggattaagc gccgcagcgc
    21181 ctttctcgat ggcttcagca gccaaaggta tatctgcggt gatcaccaaa tctcccgctt
    21241 cacactgccg gacaatttcg ttatcggcaa cgtcgaaacc tgccgcgacg cgcagcgtac
    21301 gaataaatcg cgatggcggc acgcgtaaac tctggtttgc taccagtacc agcggcatct
    21361 gcatacgttc cgccgcgcga tacaaaatct ctttaattac attgggacac gcgtcggcat
    21421 ccacccaaat tgtcataaag tcatcctttg ttgggtaatc ctctattgtg tcgcgctttt
    21481 gccttccggc atagttctgt ttatgcttct gccagcgatt atcaaaacaa tgaatttcac
    21541 ggcaggagtg aggcaatgga aaagaaaatc ggttttattg gctgcggcaa tatgggaaaa
    21601 gccattctcg gcggtctgat tgccagcggt caggtgcttc cagggcaaat ctgggtatat
    21661 accccctccc cggataaagt cgccgccctg catgacaagt tcggcatcaa cgccgcagaa
    21721 tcggcgcaag aagtggcgca aatcgccgac atcatttttg ccgccgttaa acctggcatc
    21781 atgattaaag tgcttagcga aatcacctcc agcctcaata aagactctct ggtcgtttct
    21841 attgctgcag gcgtcacgct cgaccagctt gcccgcgcgc tgggccatga ccggaaaatt
    21901 atccgcgcca tgccgaacac tcccgcgctg gttaatgccg ggatgacctc cgtaacgcca
    21961 aacgcgctgg taaccccaga agataccgct gatgtgctga atattttccg ctgctttggc
    22021 gaagcggaag taattgctga gccgatgatc cacccggtgg tcggtgtgag cggttcttcg
    22081 ccagcctacg tctttatgtt tatcgaagcg atggccgacg ccgccgtgct gggcgggatg
    22141 ccacgcgccc aggcgtataa atttgccgct caggcggtaa tgggttccgc aaaaatggtg
    22201 ctggaaacgg gagaacatcc gggggcactg aaagatatgg tctgctcacc gggaggcacc
    22261 accattgaag cggtacgcgt actggaagag aaaggcttcc gtgccgcagt gatcgaagcg
    22321 atgacgaagt gtatggaaaa atcagaaaaa ctcagcaaat cctgatgact ttcgccggac
    22381 gtcaggccgc cacttcggtg cggttacgtc cggctttctt tgctttgtag agcgccaaat
    22441 ctgccgattt caaccactcg cgatagtgac tcatttgtgg gttcagcggc gcaaccccca
    22501 cactaatccg taaatttacc tgtggcgtat tcggcaaacg taatgtattt agcccttcat
    22561 gcacccgtaa catggcggta atggcgctct cagctggcgt accggacatg attaccgcaa
    22621 actcatcgcc gccaaaccga ccaatcacat cgctaccgcg cagggtaatt tgtaactgtc
    22681 gggtaagcgc cacaatcgct tcatcgccca catcatggcc ccaggtatcg ttgatgctct
    22741 tgaaatggtc gatatcgata atcagtaacg ttgcatcgcg attatgccgc cgacagttat
    22801 caaattcatt gcgtaacata gtttcccaat gacgtcggtt atacacgccc gtcatgccgt
    22861 cgcgggtact catgacctgc aacctgcgtt tatgttccgc cagtttggtt gccgtctggt
    22921 agctgaccca gccaaacagc agaggataaa tgacaataat gggaagggag agccaccatt
    22981 ccagcggcgc actattgaac gacacggtaa tgcccgtcag ctcgagggtg acaaggcagg
    23041 aaaccaccat caacaccaga cccgcgacaa acagacgggg gccgcctgcc cccatcaaat
    23101 tcagacacat aatcatcaac atcgcggtgg aaggcagcac gtttacgccc attacgccta
    23161 cccacattcc cgctaatact gcatcggttt ttaagttgta aatttcccgg ctaagcggat
    23221 cgacggccct gctcgctatc tgccaggcta aatgcggcca gacgaacgcc cagccgacca
    23281 acaccagcca ccaccagccc ggcggcgggt gtgaaaccag cgttgaagca atcggtaaga
    23341 acatgccagc caggccaacc gcacggggta gtctgacgcg acgggcgaag cgcagcccgg
    23401 accgctgatg ttcgttttga ggagttaaag gaggttcctc cccgtgcgcc gccgcttttt
    23461 tgaaaaagtt ttcatcattc attatttttg ggaacattca gaaacaattt tcccaaatta
    23521 tagagacggg gctaaaggca gaagtatgaa atttcgggtg agccatttta gtagctcacc
    23581 cgttagcact atcaggtatc aggcggcttt cttgaggcag gcgctcataa acttattacg
    23641 atcatcacct ttcagtgatt gttgcgttgc ttgattatta cattcgcgca tcttttgctg
    23701 ctgtggcgtc aaactttttt caccaggcgc agacttgctg ttcttcaggc aatcactcat
    23761 gtaggtctta cgagcatccc ctttcaacgc ctgtgccgtc gcctgctgat tacaggaggt
    23821 catacgctgt tgttgtgggg ttaaggttct ctcggcagcg ccgacggtgg ttaaaaaaac
    23881 cagaccgaaa agcaaggtaa ccagtaatgt aattttcata gcaccatccc tcttcatgtt
    23941 ttaaccatga gcgtatgcgc ccgtgatctg acattaagtc tggttgctaa cagcaaaaaa
    24001 accacccggc agcgaaaatt cactgccggg agcggtttta tttcagcccc agagcggctt
    24061 tcatggtgta gaagagatcg gtctggtcgg tcagtccaac gacattggcg gcatgcgggc
    24121 catacgccgc aatacgcaac tgactgccgg tatgttcttg tgaatcctct tcggagttcc
    24181 cgtaactcat caccatcact gcgccatctt tggtatttag cgcctgagtg aggcccggag
    24241 ctttggtatc cggcgcaaca atctggctgg cgtgggcgtg atcagcggtg actatgacca
    24301 gcgtgttacc atcctttttg gcgaattcca gcgcccgttg tacggcttca tcgagatcga
    24361 ccgtctcgcc aatttgccca caaggattcg cagcatgatc ctgtttatcg attgacgcac
    24421 cttcaacttg caggaaaaag cctttctcat ttttactcaa caattcaatg gctttgtcgg
    24481 tcatctgtgc cagggtcggt acactgtcat tacgttgcgg attaggcgta caggtgactg
    24541 cgggcttatc gatattaccg tggtacgttg ctttcggtcc tagccagcgc actggcatat
    24601 tgccgtcagc aaacaggcca agcaggggtt tttgctgatt cgcttccgtc accgaattca
    24661 gtgaggcagc atcgctcacc aactgataac cacgcgcctg tgcctgttca cgcagcgttt
    24721 ttccctgcca ttcaccagcg gttgccgttt cagcaaaggt ttttgcgccg ccgccaagcg
    24781 taacgtcggc acgagcgtta agcagctgtt cggtaatcga tccttttccg cctttttcca
    24841 gagcgttacc cggacatttt tcactggtcg cgctcggacc gtagcatttg cgcgaggtca
    24901 catgcgccac cagcgcggcg ggcgtggcat cctgcaactc tgcggtagaa acgttaccgg
    24961 tcgccagacc tgcggctttt gccatttcca gaatcgttgg gtgatctttt tcgtgaatat
    25021 cgacgcccag cgcgccgtta taggttttga caccggttga ccaggcggtt gctgatgcag
    25081 ccgagtcggt gacgtagtcc ggttttccgg tttttttatt cagcgcatag tgagtgtatt
    25141 gcccggtaag cggtaaggca tcgatacctt taaaaaagcc gcccgcacct tcggcataat
    25201 tacgtgcggc agtaatttcc gaatccccca tcccatcgcc aatcagcaaa ataatatttt
    25261 ttgcaggttt atcgctaaga gaatcacgca gagcggcggt ctgatcagcc gttaagcggc
    25321 gagcaccgcc gggtgcagta atatcgccct gagcagcccg gttttccaga acaggcattt
    25381 ctggtgtccg ggcttttgtc acaggggtaa acagtaacgg taagagtgcc agtgcaatag
    25441 tgctttgttt cactttattt tctccatgta caaatacatt aaaaaataaa aacaaagcga
    25501 ctataagtct cggccgtgac aactttatga cagctgttga aaagattaac tttttactga
    25561 cgaggatgct tcaataactt ctttacgtaa tcgcgcagca gctccgtatc gtcgtcagga
    25621 atgctggcat cgggctttac ctcgtacagc gccccctcta cctgatcaat caaccgctgt
    25681 tggtcatttt gcgccatatt gcgaagcatt gcagtgacga taatctccaa agcttctacc
    25741 tgcgcacaca gttctttcga ctcttcttct ttttgggcaa gcttaaataa caactcagca
    25801 atgagatttt tcatgtctgt atttccttat ccaaagtatg gagaagttaa cattatgttt
    25861 cacaacttca tagtgtctgg aggtattatt tttatggcaa aggaaatact ttgcgcaaat
    25921 attttttgtt tgattaccag cgaaacgttt c
//
LOCUS       DAZFTN010000066        25719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFTN010000066 DAZFTN010000000
VERSION     DAZFTN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..157)
                     /locus_tag="ACHKV2_003021"
     CDS             complement(8..157)
                     /locus_tag="ACHKV2_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940866.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            466..1605
                     /gene="wza"
                     /locus_tag="ACHKV2_003022"
     CDS             466..1605
                     /gene="wza"
                     /locus_tag="ACHKV2_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO6940867.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1611..2054
                     /gene="wzb"
                     /locus_tag="ACHKV2_003023"
     CDS             1611..2054
                     /gene="wzb"
                     /locus_tag="ACHKV2_003023"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO6940868.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2057..4219
                     /gene="wzc"
                     /locus_tag="ACHKV2_003024"
     CDS             2057..4219
                     /gene="wzc"
                     /locus_tag="ACHKV2_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO6940869.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4312..5151
                     /gene="wcaA"
                     /locus_tag="ACHKV2_003025"
     CDS             4312..5151
                     /gene="wcaA"
                     /locus_tag="ACHKV2_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO6940870.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5154..5642
                     /gene="wcaB"
                     /locus_tag="ACHKV2_003026"
     CDS             5154..5642
                     /gene="wcaB"
                     /locus_tag="ACHKV2_003026"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO6940871.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5639..6856
                     /gene="wcaC"
                     /locus_tag="ACHKV2_003027"
     CDS             5639..6856
                     /gene="wcaC"
                     /locus_tag="ACHKV2_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO6940872.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6831..8048
                     /gene="wcaD"
                     /locus_tag="ACHKV2_003028"
     CDS             6831..8048
                     /gene="wcaD"
                     /locus_tag="ACHKV2_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO6940873.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8059..8805
                     /gene="wcaE"
                     /locus_tag="ACHKV2_003029"
     CDS             8059..8805
                     /gene="wcaE"
                     /locus_tag="ACHKV2_003029"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO6940874.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8821..9369
                     /gene="wcaF"
                     /locus_tag="ACHKV2_003030"
     CDS             8821..9369
                     /gene="wcaF"
                     /locus_tag="ACHKV2_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO6940875.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9398..10519
                     /gene="gmd"
                     /locus_tag="ACHKV2_003031"
     CDS             9398..10519
                     /gene="gmd"
                     /locus_tag="ACHKV2_003031"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6940876.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10522..11487
                     /gene="fcl"
                     /locus_tag="ACHKV2_003032"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10522..11487
                     /gene="fcl"
                     /locus_tag="ACHKV2_003032"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO6940877.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11490..11969
                     /gene="gmm"
                     /locus_tag="ACHKV2_003033"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11490..11969
                     /gene="gmm"
                     /locus_tag="ACHKV2_003033"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6940878.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11966..13189
                     /gene="wcaI"
                     /locus_tag="ACHKV2_003034"
     CDS             11966..13189
                     /gene="wcaI"
                     /locus_tag="ACHKV2_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO6940879.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     SVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13192..14628
                     /gene="cpsB"
                     /locus_tag="ACHKV2_003035"
     CDS             13192..14628
                     /gene="cpsB"
                     /locus_tag="ACHKV2_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6940880.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14821..16191
                     /gene="cpsG"
                     /locus_tag="ACHKV2_003036"
                     /gene_synonym="manB"
     CDS             14821..16191
                     /gene="cpsG"
                     /locus_tag="ACHKV2_003036"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6940881.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16246..17640
                     /gene="wcaJ"
                     /locus_tag="ACHKV2_003037"
     CDS             16246..17640
                     /gene="wcaJ"
                     /locus_tag="ACHKV2_003037"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO6940882.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17642..19120
                     /gene="wzxC"
                     /locus_tag="ACHKV2_003038"
     CDS             17642..19120
                     /gene="wzxC"
                     /locus_tag="ACHKV2_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO6940883.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19300..20580
                     /gene="wcaK"
                     /locus_tag="ACHKV2_003039"
     CDS             19300..20580
                     /gene="wcaK"
                     /locus_tag="ACHKV2_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO6940884.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20577..21797
                     /gene="wcaL"
                     /locus_tag="ACHKV2_003040"
     CDS             20577..21797
                     /gene="wcaL"
                     /locus_tag="ACHKV2_003040"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO6940885.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21808..23202
                     /gene="wcaM"
                     /locus_tag="ACHKV2_003041"
     CDS             21808..23202
                     /gene="wcaM"
                     /locus_tag="ACHKV2_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO6940886.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23360..24355
                     /locus_tag="ACHKV2_003042"
     CDS             23360..24355
                     /locus_tag="ACHKV2_003042"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO6940887.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24598..25491
                     /gene="galF"
                     /locus_tag="ACHKV2_003043"
     CDS             24598..25491
                     /gene="galF"
                     /locus_tag="ACHKV2_003043"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO6940888.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6365 a         6367 c         6925 g         6062 t
ORIGIN      
        1 aatattgtca acctaaagaa actcctaaaa accatattga atgacactta atataattct
       61 taaaaatagc caattgccga attgttacct tgcctgctat tccgttagct gtaacacttc
      121 ctcctgcatt attggagagc caatattcaa ttaccataat gtcaccttat attcggaagt
      181 taatttccgc aataaccctt tctcatcaac gattgtatgc agagagggtt tgaaaaatta
      241 agctaaatca gtgtattggt agctaaaaag ccaggggcgg tagcgtgtct ggatgcctga
      301 aagaccagtc tgaattattc tgtcaatagc ctgcggatga aatcaatttt tttaggactg
      361 atgccagtta aatttttatt caattaactg caaggtaaat gtgcatggcg acattgctat
      421 taatagtgca caggataatt actctgccaa agtgataaat aaacaatgat gaaatccaaa
      481 atgaaattga tgccattatt ggtgtcagta accttgataa gcggttgcac agtacttccg
      541 ggcagcaata tgtcgacgat gggcaaagac gtcatcaaac agcaggacgc tgatttcgat
      601 ctcgacaaaa tggtgaatgt ttatccgctg accccgcgcc tgattgacca attacgccca
      661 cgcccgaatg tagcgcgccc caatatgacg ctggaaagtg agatcgcgaa ttaccagtat
      721 cgcgtcgggc cgggggacgt tcttaatgtc accgtctggg atcacccgga actcaccacg
      781 ccagccggtc agtaccgcag ctccagcgac accggcaact gggtacagcc tgacggcact
      841 atgttttacc cgtatatcgg caaggttcac gtagtcggga aaacgctcgc tgaaatccgc
      901 agtgatatta ccgggcgctt agcgacgtac atcgctgacc cgcaggtgga cgttaatatc
      961 gccgccttcc gctcacaaaa ggcctatatc tcaggtcagg tgaataaatc tggtcaacag
     1021 gcgatcacca acgtaccact gaccattctc gacgccatca acgccgcagg tggcctgacc
     1081 gacaccgctg actggcgcaa cgtggtgcta acacacaatg gtcgtgaaga gcgaatttct
     1141 ttgcaggcgc tgatgcaaaa cggcgacctc aatcagaacc gactgcttta ccccggcgat
     1201 attctctacg tgccgcgtaa tgatgatctg aaagtgtttg tgatgggtga agtgaagaaa
     1261 cagagcaccc tgaaaatgga ctttagcggc atgaccctga ccgaagccct gggcaatgct
     1321 gaaggcatcg acatgaccac ctccaacgcc agcggcatct ttgtcattcg tccgctgaaa
     1381 ggcgagggcg ggcgtaacgg caaaattgcc aatatctacc agctggatat gtccgatgcc
     1441 acgtcgctgg tgatggcgac agaattccgc ctgcaacctt atgacgtggt gtatgtcacc
     1501 accgccccgg tttcccgctg gaaccgtctg atcaatcagt tgctgccaac tattagcggt
     1561 gtccgttaca tgacggatac agccagcgac attcataact ggtaatcgtc atgtttaaca
     1621 acatcttagt tgtctgtgtc ggcaatattt gccgttcccc gacggcggaa cgcttactgc
     1681 aacgttatca cccggagctg aaagtggagt ccgctggact cggcgcgctg gtcggtaagg
     1741 gcgctgatcc taccgctatc agcgtcgccg cagaacatca actgtctctg gaaggccact
     1801 gtgcacgaca aatcagccgc cgcctgtgtc gcaactatga cctgattttg accatggaaa
     1861 agcgccatat cgaacgctta tgcgagatgg caccggagat gcgcggcaaa gtgatgctgt
     1921 ttggtcactg ggataacgaa tgtgaaatcc ccgatccgta tcgcaaaagc cgggaaacgt
     1981 ttgcagcggt gtacacatta cttgaacggt ctgcccgcca gtgggcgcag gcattgaacg
     2041 cagagcaggt ataagaatga cagaaaaagt aaaacaacat gccgctccgg taacgggcag
     2101 tgatgaaatc gatattggtc gcctggtcgg caccgtcatt gaagcgcgct ggtgggtgat
     2161 tggcatcacc gctgtattcg ccctttgtgc cgtggtttac accttcttcg ccacgccgat
     2221 ttatagtgcc gacgcactgg tacaaatcga gcaaagcagc ggcaattcgt tagtgcagga
     2281 tatcggatcg gcgttagcca acaaaccgcc tgcatcggac gccgagatcc agttgattcg
     2341 ttcgcgcctg gtgcttggta aaacggtgga tgatctcgac ctcgatattg cggtgagcaa
     2401 aaacacgttc ccgattttcg gtgcgggctg ggatcgcctg atgggacgcc agaacgagac
     2461 ggtgaaagtg actaccttta accgtccgaa agagatggag gatcaggtgt ttacgcttaa
     2521 tgtgctggac aacaaaaact acaccctgag cagcgatggc ggctttagcg cccgtgggca
     2581 agcgggccag atactgaaaa aagaaggcgt cacgctgatg gttgaagcca ttcacgcccg
     2641 cccgggcagt gagtttaccg tcaccaaata ctccacgctg gggatgatca atcaactgca
     2701 aaacagcctg acggtaacgg agaacggcaa agacgcaggc gtactgagcc tgacttatac
     2761 cggtgaagat cgcgaacaga tccgcgacat tcttaacagc atcgcccgta actatcagga
     2821 acaaaatatt gagcgcaaat cggcggaagc gtcgaaaagc ctcgctttcc tcgcgcaaca
     2881 gttaccggaa gtacgtagcc gccttgatgt tgccgaaaac aaactgaatg ccttccgtca
     2941 ggataaagat tctgttgatc tgccgctgga agcgaaagcg gtgctcgatt cgatggtgaa
     3001 catcgacgcc cagttgaacg aactgacctt taaagaggcg gaaatctcca agctgtacac
     3061 caaagttcac cccgcgtacc gcacgctgct ggagaaacgt caggcgctgg aagacgaaaa
     3121 agccaaactt aatggtcgcg taacggcgat gccgaaaacc cagcaggaaa ttgtccgtct
     3181 gacccgcgat gtcgagtctg gtcagcaggt ctatatgcaa ctgctgaata aagagcagga
     3241 gctgaaaatc accgaggcca gcaccgtcgg cgatgtgcgc attgttgacc cggcaatcac
     3301 tcagcctggt gtgctaaaac cgaagaaagg gctgattatc cttggggcga ttatccttgg
     3361 cctgatgctc tctatcgtgg gggtgctgct gcgctcgttg tttaatcgcg gcatcgaaag
     3421 cccgcaggtg ctggaagaac acggtatcag cgtctatgcc agcatcccgc tgtcggagtg
     3481 gcagaaagcg cgcgatagcg tcaaaaccat caaagggatt aaacgctata aacagagcca
     3541 gctactggcg gtggggaatc caaccgatct ggcgattgaa gccatccgca gccttcgtac
     3601 cagtttgcac ttcgcgatga tgcaggcgca gaacaatgtg ttgatgatga ccggggttag
     3661 cccgtcaatc ggtaaaacct ttgtctgcgc caacctggcg gcggtgatca gccagaccaa
     3721 taaacgcgtg ttgttgatcg actgcgatat gcgcaaaggc tacacccatg agctgttggg
     3781 cactaataac gttaatggcc tgtcggaaat tctgattggt cagggcgata ttactacagc
     3841 tgctaaaccg acctctattg ccaaatttga cctgatcccg cgcggtcagg taccgccaaa
     3901 tccttctgaa ctgttgatga gcgaacgctt tgccgaactg gtgaactggg cgagtaaaaa
     3961 ctacgacctg gtgttgattg atacgccgcc gattctggca gtgaccgatg cggcaattgt
     4021 tggtcgtcat gtcggaacca cgttaatggt ggcgcgttat gcggtcaaca cattgaaaga
     4081 agtggaaacc agtctgagcc gctttgagca aaacggtatt ccggtgaaag gggtgattct
     4141 gaactccatc ttccgccgcg ccagcgcgta tcaggattat ggctattacg aatacgaata
     4201 taagtcggat gcgaaataac gaggcctgca ttcgcaccgc cccgtaggcc ggataaggcg
     4261 ctcacgccgc atccggcaag caaaccagct cataagccgg gagtacaacc tatgaaagac
     4321 aatccgctga tctcaatcta tatgccgacc tggaaccgcc aacaactggc gattcgggcg
     4381 ataaaatcgg tcttgcgcca ggactacagc aactgggaga tgatcatcgt ggatgattgc
     4441 tccacctcct gggaacaact gcaacagtac gtcaccgccc tcaacgatcc gcgtattacc
     4501 tacattcaca acgacattaa cagcggggcg tgcgcggtac gtaaccaggc gattatgctg
     4561 gcgcaagggg aatacattac cgggatcgat gacgatgatg aatggacgcc caaccgcctg
     4621 agcgtcttcc tcgcccataa acagcaactg gtcactcacg cctttttgta cgctaacgat
     4681 tacgtttgcc agggcgaagt ttattcccaa ccggcaagct taccactgta tcccaaatcg
     4741 ccgtactcac gccgcctgtt ctataaacgc aatatcattg gcaatcaggt ctttacctgg
     4801 gcatggcgtt tcaaagagtg tttgttcgat accgaactga aagcggcaca ggattacgac
     4861 atcttcctgc ggatggtggt ggagtatggc gaaccgtgga aagtggaaga ggcgacgcag
     4921 atcctgcata tcaatcacgg tgaaatgcag atcacctcgt caccgaaaaa attctccgga
     4981 tacttccact tttaccgcaa gcacaaagac aaattcgacc gtgccagtaa aaaatatcag
     5041 ctgtttaccc tctaccagat ccgcaataaa cgtatgacct ggcgcacttt gctgacgctg
     5101 ctgtcggtgc gtaacggcaa acgtctggct gatggtattc gggggcgcta aatatgctgg
     5161 aagatctacg cgccaacagc tggagtttac gcccgtgctg catggttctt gcctatcgcg
     5221 tcgctcattt ttgttcggtg tggcgcaaaa agaacgttct caacaatctt tgggcggccc
     5281 cgctgctggt gctgtatcgc attatcaccg aatgcttttt cggttatgaa atccaggctg
     5341 ccgcgaccat tggccgccgc tttactatcc atcacggtta cgccgtagtg atcaataaaa
     5401 acgtggtagc gggggatgat ttcaccattc gccacggcgt cactatcggc aatcgtggtg
     5461 ccgataacat ggcatgtcca cacattggca acggcgtcga actcggtgcc aacgtcatta
     5521 ttcttggtga tatcacgctt ggtaacaacg tcaccgtggg cgcgggcagc gtggtgctcg
     5581 actctgtccc ggacaacgcg ctggtggtgg gagaaaaagc gcgagtgaag gtaattaaat
     5641 gaatattctg caatttaatg tgcgactggc ggaaggcggg gcggcaggtg tggcgttaga
     5701 tctccaccag cgtgcgctgc aacaggggct ggcgtcacat tttgtctacg gctacggcaa
     5761 aggcggcaaa gagagcgtca gccatcagaa ctatccgcag gtcatcaaac atacgccgcg
     5821 gatgaccgca atggcgaata ttgccctgtt tcgtctgttt aatcgcgatc tgtttggcaa
     5881 tttcaatgag ttatatcgca ccattactcg tacaccgggt ccggtggtcc tgcattttca
     5941 tgtgctgcac agctactggc taaatcttaa gagcgtggtg cgcttttgcg aaaaggtgaa
     6001 aaaccacaaa ccggatgtca ctctggtctg gacgctgcac gaccactgga gcgttaccgg
     6061 gcgctgcgcc tttaccgacg gttgtgaagg ctggaaaacg ggctgccaga aatgcccgac
     6121 cttaaataac tatccgccgg tgaagattga tcgggcgcac cagctggtgg cgggcaaacg
     6181 ccagttattc cgtgagatgc tggcgctggg ctgtcagttt atttccccca gccagcatgt
     6241 ggctgacgct ttcaatagcc tgtacggtcc agggcgttgc cggattatca ataacggtat
     6301 tgatatggca accgaggcga ttctggcgga tttgcctccg gtgcgcgaaa ctcagggcaa
     6361 gccgaaaatc gcggtggtag cgcatgacct gcgttacgac ggcaaaacta accagcaact
     6421 ggtacgcgag atgatggcgc tgggcgacaa aatcgaactg catacctttg gtaagttctc
     6481 gccgttcacc gctggcaacg tggttaatca cggctttgaa actgacaagc gcaagttgat
     6541 gagcgcgctc aatcagatgg atgcgctggt gttcagttct cgcgtcgata actacccgct
     6601 gattttgtgt gaggcgttat caattggcgt gccggtgatt gccacccata gcgacgcggc
     6661 gcgtgaagtg ttgcaaaaat ccggcggtaa aaccgtcagc gaagaagagg tgctgcaact
     6721 ggtccagtta agcaaaccgg aaatcgcgca ggcgttattt ggtaccacgc tggctgagtt
     6781 cagccaacgc agccgcgccg cctacagtgg acaacagatg ctggaggagt atgtcaactt
     6841 ctatcagaat ctgtagctac ctgctgctgc cgctgatcta cctgctggtt aacgtcaaaa
     6901 tcacccagct tggcgaaagt ttccccatca ccatcgtcac ttttttacct gtcttgttac
     6961 tgctgttttt agaacgcatc agcgttaaaa aattgatgat tgccttaggg atttgcgcgg
     7021 gactcacggc gtttaactac ctgtttggtc agtcgctgga tgccagtaaa tacgtcactt
     7081 caactatgct gtttgtctat atcgtgatca ttattggtat ggtgtggagt attcgcttta
     7141 aaacgatttc gccacacaac catcgcaaga tattacgttt cttttatctg gtggtcgggc
     7201 tggtggtggc gctggcggcg gtggagatgg cgcaaattat cctcaccggc ggcagcagta
     7261 ttatggagtc gatttcgaaa tatctgattt acagcaacag ctatgtgctg aatttcatca
     7321 aattcggcgg caagcgcacg acagcacttt atttcgaacc ggcatttttc gctctggcat
     7381 taatctcaat ttggctcagc atcaaacagt ttggtatcaa aacgcctaaa acagatgcta
     7441 tgattctcgc agggataata ttatccggat cgttttcagg ggttatgacc tttatcctgt
     7501 tttatttgct ggagtgggca tttcaatatc tgaataaaga ggcgattaag aaaaagttac
     7561 cgttagcatt gatttctctg gcagtattcc tggttggtgt ggtaattgca tttccttata
     7621 tttccacccg tctgggcgat ttaggtacgg aaggatcgtc atcgtattat cgtattgtcg
     7681 gtccgctggt gatggtcggt tattctttga cccatattga cggtgtagtc agatttggct
     7741 cactttatga atatgtcgca tcattcggaa tatttaacgg tgcggatgtc ggaaaaacca
     7801 tagacaatgg tttgtatctg ctgattattt atttttcttg gttcgcggtg tttttatcgc
     7861 tgtggtacat ggggaaagtg ataaaaatga tgatcaacgc ttttggtgat aaccgcaatt
     7921 ttcgcgtgca attatatctt tttactccgg tatcgctgtt ttttaccggt tcgatattta
     7981 gcccggaata tgcattttta atcgtctgtc cgtttatttt gcgaaaagcg ttaaatatta
     8041 cgaggtaaga ataagaacat gttgcttagc ataatcactg tcgcgtttcg taacctcgaa
     8101 gggatagtca aaacacatgc ctcgctggcg catctggcgc aggcggaaga tatcagcttc
     8161 gaatggattg ttgtcgatgg cggttccaat gacggcactc gtgagtacct ggaaaatctc
     8221 aatggtatct ataacctacg ctttgtcagc gagccagata acggtatcta cgacgccatg
     8281 aacaagggca ttgcgatggc acaaggcaag ttcgcgttgt ttctcaactc gggcgatatt
     8341 tttcatcagg atgccgcata ttttgtccgt aagttaaaaa tgcaaaaaga taacgtgatg
     8401 atcaccggcg atgcgctgct ggattttggc gacgggcata aaattaaacg tagcgccaaa
     8461 ccgggctggt atatttatca cagcctgccc gccagtcatc aggcgatatt tttcccggta
     8521 tccggtttga aaaaatggcg ttatgacctg gaatataaag tttcttctga ttacgcgctg
     8581 gcagccaaaa tgtataaagc aggttatgca tttaaaaaac tcaatggcct ggtgtctgaa
     8641 ttttctatgg gtggggtatc taccaccaat aatatggaat tgtgtgctga cgcgaaaaaa
     8701 gtccaacggc aaatattaca tgtgcctggc ttttgggctg aattatcctg gcatttacgc
     8761 cagcgtacta cctcaaagac gaaagcctta tataacaaaa gctgaatata agggaaaacg
     8821 atgcaggatt taagcggttt ctcggtgccg aaagggttcc ggggcggcaa cgctattaaa
     8881 gtgcaattgt ggtgggcagt acaggcaaca atatttgcct ggtcgccaca agtattgtat
     8941 cgctggcggg catttttatt acgtttattc ggcgcaaaaa taggaaaaaa cgtagttatt
     9001 cgtccgtcag taaaaattac ctatccgtgg aaattaacct taggtgatta cgtgtgggtc
     9061 ggcgatgacg tcaatttata taccctcggt gaaataacca ttggcgcaca ttcggtgata
     9121 tcgcaaaaaa gttatttatg caccgggagc cacgaccatg caagtcaaca tttcaccatt
     9181 aacgccacgc ctattgtgat tggcgagaaa tgctggctgg caaccgatgt ttttgttgcc
     9241 ccaggcgtca cgattggcga cggcaccgtc gtgggtgcgc gaagcagtgt ttttaaatcg
     9301 cttccggcaa atgtggtttg ccgggggaat cccgcagtgg tgatacgcaa acgcgttgaa
     9361 actgaataaa ttcaaaaaaa atacagagga ataagacatg tcaaaagtcg ctctcatcac
     9421 cggtgtaacc ggacaagacg gttcttacct ggcagagttt ctgctggaaa aaggttacga
     9481 ggtgcatggt attaagcgtc gcgcatcgtc attcaacacc gagcgcgtgg atcacattta
     9541 tcaggatccg cacacctgca acccgaaatt ccatctgcat tatggcgacc tgagtgatac
     9601 ctccaacctg acgcgtattt tgcgtgaagt gcagccggat gaagtgtaca acctgggcgc
     9661 aatgagccac gttgcggttt cttttgagtc accggaatat accgctgacg tcgacgcgat
     9721 gggtacgctg cgcctgctgg aggcgatccg cttcctcggt ctggaaaaga aaacccgttt
     9781 ctatcaggct tccacctctg aactgtatgg tctggtgcag gaaattccgc agaaagagac
     9841 cacgccgttc tatccgcgct ctccgtatgc ggtcgccaaa ctgtatgcct actggatcac
     9901 cgtcaactac cgtgaatcct acggcatgta tgcctgtaac ggtattctct tcaaccatga
     9961 atccccgcgc cgcggcgaaa ccttcgttac ccgcaaaatc acccgcgcaa tcgccaacat
    10021 cgcccagggg ctggagtcgt gcctgtacct cggcaatatg gattccctgc gtgactgggg
    10081 ccatgccaaa gactacgtaa aaatgcagtg gatgatgctg caacaggaac agccagaaga
    10141 tttcgttatc gcgaccggcg ttcagtactc cgtacgtcag ttcgtagaaa tggcggcagc
    10201 acagctgggc atcaaactgc gctttgaagg cacgggcgtt gaagagaagg gcattgtggt
    10261 ttccgtcacc gggcatgacg cgccgggtgt taaaccgggt gatgtgatta tcgccgttga
    10321 tccgcgttac ttccgtccgg ctgaagttga aacgctgctc ggcgacccga ccaaagcgca
    10381 cgaaaaactg ggctggaaac cggaaatcac cctcagagag atggtgtctg aaatggtggc
    10441 taatgacctc gaagcggcga aaaaacactc tctgctgaaa tctcacggct acgacgtggc
    10501 gatcgcgctg gagtcataag tatgagtaga caacgcattt ttattgctgg ccatcgcggg
    10561 atggtcggtt ccgccatcag gcggcagctc gaacagcgcg gtgatgtgga actggtatta
    10621 cgcacccgcg acgagctgaa cctgctggac agccgcgccg tgcatgattt ctttgccagc
    10681 gaacgtattg accaggtcta tctggcggcc gcgaaagtgg gcggcattgt tgccaacaac
    10741 acgtatccgg cggatttcat ctatcagaac atgatgattg agagcaacat cattcacgcc
    10801 gcgcatcaga acgacgtgaa caaactgctg tttctcggat cgtcctgcat ctacccgaaa
    10861 ctggcaaaac agccgatggc agaaagcgag ttattgcagg gcacgctgga gccgaccaac
    10921 gagccttacg ccattgccaa aatcgccggg atcaaactgt gcgaatcata caaccgccag
    10981 tacggacgcg attaccgctc agtcatgccg accaacctgt atggcccgca tgacaacttc
    11041 cacccgagta attcgcatgt gatccctgca ttgctgcgtc gcttccacga ggcgacggca
    11101 cagaatgcac cggacgtggt ggtgtggggc agcggtacgc cgatgcgaga atttctgcac
    11161 gtcgatgata tggcggcggc gagcattcat gtcatggagc tggcgcatga agtctggctg
    11221 gagaacaccc agccgatgct gtcgcacatt aacgtcggca cgggcgttga ctgcactatc
    11281 cgcgagctgg cgcaaaccat cgccaaagtg gtgggttaca aaggtcgggt ggtttttgat
    11341 gccagcaaac cggatggtac gccgcgcaaa ctgctggatg tgacgcgcct gcatcagctt
    11401 ggctggtatc acgaaatctc actggaagcg gggcttgcca gcacttacca gtggttcctt
    11461 gagaatcaag accgctttcg ggggtaatga tgtttttacg tcaggaagac tttgccacgg
    11521 tagtgcgctc cacaccgctt gtctctctcg actttattgt cgagaacagt cgcggcgagt
    11581 ttctgcttgg caaaagaacc aaccgcccgg cgcagggtta ctggtttgtg ccgggagggc
    11641 gcgtgcagaa agacgaaacg ctggaagccg catttgagcg gctgacgatg gcggaactgg
    11701 ggctgcgtct gccgataaca gcaggccagt tttacggtgt ctggcagcac ttttatgacg
    11761 ataacttctc cggcacggat ttcaccactc actatgtggt gctcggtttt cgcttcagag
    11821 tagcggaaga agaactgtta ctgccggatg agcagcatga cgattaccgc tggctgacgc
    11881 cggacgcgct gctcgccagt aacgatgttc acgctaacag ccgcgcctat tttctcgctg
    11941 agaagcgtgc cggagtaccc ggattatgaa aatactggtc tacggcatta actactcgcc
    12001 ggagttaacc ggcatcggca aatacaccgg cgagatggtg gaatggctgg cggcgcaagg
    12061 tcatgaggtg cgggttatta ccgcaccgcc ttactacccg cagtggcaag tgggcgagaa
    12121 ctattccgcc tggcggtaca aacgagaaga gggggccgct acggtgtggc gctgcccgct
    12181 gtacgtgcca aaacagccga gcaccctgaa acgcctgttg catctcggca gttttgccgt
    12241 cagcagtttt ttcccgttga tggcgcaacg tcgctggaag ccggatcgca ttatcggcgt
    12301 agtgccaacg ctgttttgca cgccgggaat gcgcctgctg gcgaaactct ctggcgcgcg
    12361 taccgtgctg catattcagg attacgaagt agatgccatg ctggggctgg gccttgccgg
    12421 aaaaggcaaa ggcggcaaag tggcacagct ggcaacggcg ttcgaacgta gcggactgca
    12481 taacgtcgat aacgtttcca cgatttcgcg ttcgatgatg aataaagcca tcgaaaaagg
    12541 cgtggcggcg gaaaacgtca tcttcttccc caactggtcg gaaatcgccc gttttcagca
    12601 tgttgcagac gccgatgttg atgcccttcg taaccagctt ggcctgccgg ataacaaaaa
    12661 aatcattctt tactccggca atattggtga aaagcagggg ctggaaaacg ttattgaagc
    12721 agccgatcgc ctgcgcgatg aaccgctgat ttttgccatt gtcgggcagg gcggcggcaa
    12781 agcgcggctg gaaaaaatgg cgcagcagcg tggactgcgc aacatgcaat ttttcccgct
    12841 gcaatcgtat gacgctttac ccgcactgct gaagatgggc gattgccatc tggtggtgca
    12901 aaaacgcggc gcggcagatg ccgtattgcc gtcgaaactg accaatattc tggcagtagg
    12961 cggtaactcg gtgattactg ctgaagccca cacagaactg ggacagcttt gcgaaacctt
    13021 tccgggcatt gcggtttgcg tagaaccgga atcggttgag gcgctggtgg cggggattcg
    13081 tcaggcgctc ctgctgccca aacacaacac ggtggcacgt gaatatgccg aacgcacgct
    13141 cgataaagag aacgtgttac gtcaatttat aaatgatatt cggggataat tatggcgcag
    13201 tcgaaactct atccagttgt gatggcaggt ggctccggta gccgcttatg gccgctttcc
    13261 cgcgtacttt atcccaagca gtttttatgc ctgaaaggcg atctcaccat gctgcaaacc
    13321 accatctgcc gcctgaacgg cgtggagtgc gaaagccctg tggtgatttg caatgagcag
    13381 caccgcttta ttgttgcgga acaactgcgt caactgaaca aactcactga gaacattatt
    13441 ctcgaaccgg cagggcgtaa cacagcaccg gccattgcgc tggcggcgct ggcggcaaaa
    13501 cgtcatagcc cggagaacga cccattaatg ctggtgctgg cggcggatca tgtgattgcc
    13561 gatgaagacg cattccgcgc cgccgtgcgt aacgctatgc cgtatgccga agcgggcaag
    13621 ctggtgacct tcggcattgt gccggatcta cctgaaaccg gatatggcta tattcgtcgc
    13681 ggtgaagtgt cggcgggtga gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa
    13741 aaaccaaatc tggaaaccgc gcaggcctat gtggcaagcg gcgaatatta ctggaacagc
    13801 ggtatgttcc tgttccgtgc cggacgctat ctcgaagaac tgaaaaaata tcgcccggat
    13861 attctcgacg cctgtgaaaa agcgatgagc gccgtcgatc cggatctcga ttttattcgc
    13921 gtggatgaag acgcgtttct ggcctgcccg gaagagtcgg tggattacgc ggtcatggaa
    13981 cgtacggcag atgccgttgt ggtgccgatg gatgcgggct ggagcgatgt cggctcctgg
    14041 tcctcattat gggagatcag cgcccacacc gccgagggca acgtttgcca cggcgatgtg
    14101 attaatcaca aaactgaaaa cagctatgtg tatgccgaat ctggcctggt caccaccgtc
    14161 ggggtgaaag atttggtggt agtgcagacc aaagatgcgg tgctgattgc cgaccgtaac
    14221 gcggtgcagg atgtgaaaaa agtggtcgag cagatcaaag ccgatggtcg ccatgagcat
    14281 cgggtgcatc gcgaagtcta tcgcccgtgg ggcaaatatg actctatcga cgcgggcgac
    14341 cgctaccagg tgaaacgcat caccgtgaaa ccgggcgaag gcttgtcggt acagatgcac
    14401 catcaccgcg cggaacactg ggtggttgtc gcgggaacgg caaaagtcac cattgacggt
    14461 gatatcaaac tgcttggtga aaacgagtcg atttatattc cgctgggggc gacgcactgc
    14521 ctggaaaacc cggggaaaat tccgctcgat ttaattgaag tgcgctccgg ctcttatctc
    14581 gaagaggatg atgtggtgcg cttcgcggat cgctacggac gggtgtaaac gtcgcatcag
    14641 gcgctgattg tcggatgcgg cgtgaacgcc ttatccgacc tacggttcgg ttttgtaggc
    14701 ctgataagac gcggcagcgt cgcatcaggc aatgaatgcg aaaccgcggt gtaaataacg
    14761 acaaaaacaa aattggccgt ttcggtcagg gccaactatt gcctgaaaaa gggtaacgat
    14821 atgaaaaaat taacctgctt taaagcctat gatattcgcg ggaaattagg cgaagaactg
    14881 aatgaagata ttgcctggcg cattggtcgt gcctatggcg aatttctcaa accgaaaacc
    14941 attgtgttag gcggtgatgc ccgtctcacc agcgaaacct taaaactggc gctggcaaaa
    15001 ggtttacagg atgcgggcgt tgacgtgctg gatattggta tgtccggcac cgaagagatc
    15061 tatttcgcca cgttccatct cggcgtggat ggcggcattg aagttactgc cagccataac
    15121 ccgatggatt acaacggcat gaaactggtg cgcgaagggg ctcgcccgat cagcggcgat
    15181 accggactgc gcgatgtcca gcgtctggca gaagccaacg actttcctcc cgttgatgaa
    15241 accaaacgcg gtcgctatca gcaaatcaat ctgcgtgacg cttacgttga tcacctgttc
    15301 ggttatatca acgtcaaaaa ccttacgccg ctcaagctgg tgatcaactc cgggaacggc
    15361 gcggcgggtc cggtggtgga cgccatcgaa gcccgcttta aagccctcgg cgcaccggtg
    15421 gaattgatca aagtgcacaa cacgcccgac ggcaatttcc ccaacggtat tcctaacccg
    15481 ctgctgccgg aatgtcgcga cgacacccgc aatgcggtca ttaaacacgg cgcggatatg
    15541 ggcattgctt ttgatggcga ttttgaccgc tgtttcctgt ttgacgaaaa agggcagttt
    15601 atcgagggct actacattgt cggtctgctg gcagaagcgt tcctcgaaaa aaatcccggc
    15661 gcgaagatca tccacgatcc acgtctctcc tggaataccg ttgatgtggt gactgccgca
    15721 ggcggcaccc cggtaatgtc gaaaaccgga cacgccttta ttaaagaacg gatgcgcaag
    15781 gaagacgcca tctacggtgg cgaaatgagc gcccaccatt acttccgtga ttttgcttac
    15841 tgcgacagcg gcatgatccc gtggctgctg gtcgccgaac tggtgtgcct gaaaggaaag
    15901 acgctgggcg aactggtgcg cgaccggatg gcagcgtttc cgtcaagcgg tgagatcaac
    15961 agcaaactgg cgcaacccgt tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag
    16021 gcgctggcgg tggatcgcac cgatggcatc agcatgacct ttgccgactg gcgctttaat
    16081 ctgcgctcct ccaacaccga accggtggtg cggttgaatg tggaatcacg cggtgatgtg
    16141 ccgctgatgg aagcgcgaac gcgaactctg ctgacgttgc tgaacgagta atgtcggatc
    16201 ttcccttacc ccattgcggg taaggggcta ataacaggaa caacgatgac aaatctaaaa
    16261 aagcgcgagc gagcgaaaac caatgcatcg ttaatctcta tggtgcaacg cttttcagat
    16321 atcaccatca tgtttgccgg actatggctg gtttgcgaag tgagcggact gtcattcctc
    16381 tacatgcacc tgttggtggc gctgattacg ctggtggtgt tccagatgct gggcggcatc
    16441 accgattttt atcgctcatg gcgcggtgtt cgggcagcga cagaatttgc cctgttgcta
    16501 caaaactgga ccttaagcgt gattttcagc gccggactgg tggcgttcaa caatgatttc
    16561 gacacgcaac tgaaaatctg gctggcgtgg tatgggctga ccagtatcgg actggtggtt
    16621 tgccgttcat gtattcgcat tggggcgggc tggctgcgta atcatggcta taacaagcgc
    16681 atggtcgcgg tggcggggga tttagccgcc gggcaaatgc tgatggagag cttccgtaac
    16741 cagccgtggt tagggtttga agtggtgggc gtttaccacg acccgaaact gggcggcgtt
    16801 tctaacgact gggcgggtaa cctgcaacag ctggtcgagg atgctaaagc aggcaagatt
    16861 cataacgtct atatcgcgat gcaaatgtgc gacggcgcgc gagtgaaaaa actggtccat
    16921 caactggcgg acaccacctg ttcggtgctg ctgatccccg atgtctttac cttcaacatt
    16981 ctccattcac gtcttgaaga gatgaacggc gttccggtgg tgccgcttta cgacacgccg
    17041 ctttccgggg ttaaccgcct gctcaaacgt gcggaagaca ttgtgctggc gacgcttatc
    17101 ctgctgctga tctccccggt gctgtgctgc attgcgctgg cggtgaaact cagttcacct
    17161 gggccggtta ttttccgcca gactcgctac ggcatggatg gcaagccgat caaagtgtgg
    17221 aagttccgtt ccatgaaagt gatggagaac gacaaagtgg tgacccaggc gacgcagaac
    17281 gatccgcgcg tcaccaaagt ggggaacttt ctgcgccgca cctcgctgga tgaattgccg
    17341 cagtttatca atgtgctgac cggggggatg tcgattgtcg gtccacgtcc gcacgcggtg
    17401 gcgcataacg aacagtatcg acagctcatt gaaggctaca tgctgcgtca taaggtgaaa
    17461 ccgggcatta ccggctgggc gcagattaac ggctggcgcg gcgaaaccga cacgctggag
    17521 aaaatggaaa aacgcgtcga gttcgacctt gagtacatcc gcgaatggag cgtctggttc
    17581 gatatcaaaa tcgttttcct gacggtattc aaaggattcg ttaacaaagc ggcatattga
    17641 tatgagctta cgtgaaaaaa ccatcagcgg cgcgaagtgg tcggcgattg ccacggtgat
    17701 catcatcggc ctcgggctgg tgcagatgac cgtgctggcg cggattatcg acaaccacca
    17761 gttcggtttg cttaccgtgt cgctggtgat catcgcgctg gcagatacgc tttctgactt
    17821 cggtattgct aactcgatta ttcagcgaaa agaaatcagc caccttgaac tcaccacgct
    17881 gtactggctg aacgttgggc tggggcttgt ggtgtgtgtg gcggtgtttt tgttgagtga
    17941 tctcattggt gacgtgctga acaacccgga cctggcaccg ttgattaaaa cattgtcgct
    18001 ggcgtttgtg gttatccccc acgggcaaca gttccgcgcg ttgatgcaaa aggagctgga
    18061 gttcaacaaa atcggcatga tcgaaaccag cgcggtactg gcgggcttca cttttacggt
    18121 ggttagcgcc catttctggc cgctggcgat gaccgcgatc ctcggttatc tggtcaatag
    18181 tgcggtgaga acgctgctgt ttggctactt tggccgcaaa atttatcgcc ccggtctgca
    18241 tttctcgctg gcgtctgttg cgccgaactt acgctttggt gcctggctga cggcggacag
    18301 catcatcaac tatctcaata ccaacctttc aacgctggtg ctggcgcgta ttctcggcgc
    18361 gggcgtggca gggggataca acctggcgta caacgtggcc gttgtgccgc cgatgaagct
    18421 gaacccgatc atcacccgtg tgttgtttcc ggcattcgcc aaaattcagg acgataccga
    18481 aaagctgcgc gtcaacttct acaagctgct gtcggtggtg gggattatca actttccggc
    18541 gctgctcggg ctgatggtgg tgtcaaataa ctttgtaccg ctggtatttg gcgaaaagtg
    18601 gaacagcatt attccggtgc tgcaattgct gtgtgtggtg ggtctgctgc gctccgtagg
    18661 taacccgatt ggttcgctgc tgatggcgaa agcgcgggtc gatatcagct ttaaattcaa
    18721 cgtattcaaa acatttctgt ttattccggc gattgttatt ggtgggcaga tggcgggcgc
    18781 aatcggcgtc acgctcggtt tcctgctggt gcagattatc aacaccattc tgagttactt
    18841 cgtgatgatt aaaccggttc ttggttccag ttatcgccag tacatcctga gtttgtggct
    18901 gccgttttat ctctcgctgc cgacgctggt ggtcagttat gtgctgggca ttgtgctgaa
    18961 agggcaactg gcgctgggga tgctgctggc ggtgcaaata gccgcgggcg tgctggcgtt
    19021 tgtggtgatg attgtgctgt cgcgccatcc gctggtggtg gaagtgaagc gccagttttg
    19081 tcgcagcgaa aaaatgaaaa tgcttttacg ggcggggtga gttgttgttt tcggttccga
    19141 caacggttgc cgggtgcagc gtaaaacacc ttatccatcc tacggttctg tgctcgggtc
    19201 ttgtaggcct gataagacgc gccagcgtcg catcaggcaa aaaacggcaa cgaacacaac
    19261 aaacacagcc tcacctggca tcaaatttat gaggaaaaaa tgaaattact tattctgggc
    19321 aaccacactt gcggcaatcg tggcgacagc gccatcttgc gcggcttact tgatgccatc
    19381 aacatcctca atccacatac cgaagtggac gtgatgagcc gctatccggt cagttcttcc
    19441 tggctgctca accgcccggt aatgggtgat ccgctgttcc ttcaaatgaa acaacacaac
    19501 agcgcggcgg gcgttgtcgg gcgcgttaaa aaagtcctcc gtcgccgcta tcagcaccag
    19561 gtattgctct cacgcgtcac cgacactggc aagctgcgca atatcgccat cgcccaggga
    19621 ttcaccgact tcgtgcgcct actgtcaggt tacgacgcca ttattcaggt cggcggatcg
    19681 ttttttgtcg atctctacgg cgtgccgcag tttgaacatg cactttgcac atttatggcg
    19741 aaaaagccgc tgtttatgat tggtcacagc gtcggtccct tccaggatga gcaatttaac
    19801 caactggcga actacgtttt tggtcactgc gacgcgctga tcttgcgcga atcggtcagc
    19861 ctcgatctaa tgaaacgcag caatatcacc actgcaaaag ttgaacatgg cgtcgatacc
    19921 gcgtggctgg tcgatcacca cacagaagac ttcaccgcca gctatgccgt ccaacactgg
    19981 ctggacgttg ccgcacaaca gaaaacggtg gcaattaccc tgcgcgaact ggcaccgttc
    20041 gacaaacgcc tcggcaccac tcagcaagtg tatgaaaaag cctttgccgg ggtggtcaat
    20101 cgcattctcg acgaaggcta tcaggtcatt gcgctctcca cctgtacggg tatcgacagc
    20161 tataacaaag acgaccgcat ggtggcgctc aacctgcgcc agcacatcag cgatcctgcc
    20221 cgttatcacg tagtgatgga tgaacttaac gatctggaaa tgggcaaaat tctcggtgcc
    20281 tgtgaactca ccgtcggtac gcgcctgcac tccgccatta tctcaatgaa ctttgctact
    20341 ccggcgattg ccatcaacta tgaacataaa tccgccgggg ttatgcagca gctgggacta
    20401 ccggagatgg caattgatat ccgtcattta ttagacggca gcctgcaagc gatggttgcg
    20461 gataccttag gccagcttcc ggcgctgaac gcacgactta gcgaagccgt cagtcgtgag
    20521 cgtcagacgg gaatgcagat ggtgcagtcc gtactggaac gcatcgggga ggtgaaatga
    20581 aggtcggctt cttcttattg aaatttccgc tgtcgtcaga aaccttcgtt cttaaccaaa
    20641 tcactgcgtt tattgatatg ggctttgaag tggagattgt cgcgctgcaa aaaggcgaca
    20701 cacaaaacac ccacgtggca tggactaaat acaaccttgc cgccagaacc cgctggttac
    20761 aggacgaacc tacgggcaaa gtggcgaaac tgcgccaccg agccagtcag accttgcgcg
    20821 gcattcatcg taaaaatacc tggcaggcgc tcaacctcaa acgctatggt gccgagtcgt
    20881 ggaacctgat tttgtctgcc atttgcggcc aggtcgcaac accgtttcgc gccgatgtgt
    20941 tcatcgctca ttttggccct gcgggggtaa ccgcagcaaa actacgcgaa ctgggtgtca
    21001 ttcacggcaa aattgccact attttccacg gcattgatat ttccagtcgg gaagtgctca
    21061 accattacac tcccgaatat caacaactgt ttcgacgtgg cgacctgatg ctaccgataa
    21121 gcgatctgtg ggccggaagg ctgcaaaaaa tgggctgccc gagggaaaaa atcgccgtat
    21181 cgcgcatggg cgtagatatg acgcgcttta gcccgcgtcc cgtgaaagcg cccgcaacac
    21241 cgctggagat tatttccgtc gcacgcttaa ccgagaaaaa aggtctgcat gtggcgattg
    21301 aagcctgccg gcagttgaaa gagcagggcg tggcatttcg ctatcgcatc ctcggcattg
    21361 gcccgtggga acgacgcctg cgcacgctca tcgaacaata tcaactggaa gatgtggtgg
    21421 agatgccggg ctttaaaccg agccatgaag tgaaagcgat gctcgacgac gcggatgtct
    21481 tcctgttgcc atcggttaca ggtgcggatg gcgatatgga aggcattccg gtggcgctga
    21541 tggaggcgat ggcggtcggc attccggtgg tttctactct gcatagcgga ataccggaac
    21601 tggtggaggc tgacaaatcc ggctggctgg tgcctgagaa cgatgctcgc gcactggcgc
    21661 aacgactggc ggcgtttagc caactggaca ccgacgaatt ggctccggtc gtcaaacgcg
    21721 cgcgcgaaaa agttgaacac gattttaacc agcaggtgat caatcgagaa ctcgccagct
    21781 tgctgcaggc tttatagagg agaacgtatg ccatttaaaa aactctcccg acgcaccttc
    21841 ctgacggcaa gctcggcgct tgccttcctt catacccctt tcgctcgcgc acttcccgcc
    21901 cgacaaagcg ttaatatcaa cgactacaac cctcacgact ggatcgcctc atttaaacaa
    21961 gccttcagcg aagggcaaac ggttgtcgtg cctgctggat tcgtttgtga caatatcaac
    22021 accggcatct tcattcctcc tggcaaaacg ttacacatcc ttggaagcct gcgcggcaac
    22081 ggcagagggc gatttgtctt acaggacggc agccaggtga caggggagga gggcggcagt
    22141 atgcataaca tcaccctgga tgtgcgtggt tctgactgca ccatcaaagg gctggcgatg
    22201 agcggctttg gtccggtaac gcagatttat atcggcggca aaaacaaacg ggtcatgcgc
    22261 aacctgacca tcgataacct gaccattagc cacgctaatt acgccatctt acgccaggga
    22321 tttcataacc agattatcgg tgccaacatc accaattgta agttcagcga cttacagggc
    22381 gatgccattg aatggaacgt ggcgattaac gaccgcgata ttttgatctc cgaccatgtc
    22441 atcgagcgca tcaactgtac taacggcaaa atcaactggg gcattggcat aggccttgcg
    22501 ggaagcactt acgataataa ttacccggaa gatcaggcag tgaaaaactt tgtcgtggcg
    22561 aatatcacgg gatcggattg tcggcagttg atacatgttg aaaatggtaa acattttgtt
    22621 attcgtaata tcaaagcccg caatatcacg caggatttca gtaagaaagc aggtattgat
    22681 aacgccacag tcgctattta cggttgtgac aatttcgtga ttgataatat tgaaatgatt
    22741 aatagcgccg ggatgttaat cggctatggg gtaattaaag gcaattatct ctcgattccg
    22801 caaaatttcc gagtgaataa tattcaactg gataacaccc atcttgctta taaattgcgc
    22861 ggcctccaaa tctctgccgg gaatgccgtc tcctttgtgg cactaactaa cattgagatg
    22921 aagcgtgcgt cgctggagtt acacaacaaa ccgcaacatc tttttctgcg taatatcaaa
    22981 gtgatgcagg aatcctctgt tggacccgca ttgattatga acttcgacat gcgcaaagac
    23041 gttcgaggcg tctttatggc gaaagaagaa acactgctgt ctcttgcaaa tgttcatgcg
    23101 gtgaatgaga aaggacaaag ctccgtcgat atcgacagga ttaatcacca tattgttaat
    23161 gtggaaaaga ttaactttag attgccggaa cggagagagt agatttgcga ccattcataa
    23221 acttactgga tataatgttc taaaagatta taatcctcac actaactgtt aaagctgggg
    23281 ctgggcagca ttgactactc agggaaggta ctcatcttta tcttgtgttt cttacgtaac
    23341 aaccagacta aagtgattta tgaacgataa cgttttgctc ataggagctt ccggattcgt
    23401 aggaacccga ctacttgaaa cggcaattgc tgactttaat atcaagaacc tggacaaaca
    23461 gcagagccac ttttatccag aaatcacaca gattggtgat gttcgtgatc aacaggcact
    23521 cgaccaggcg ttagccggtt ttgacactgt tgtactactg gcagcggaac accgcgatga
    23581 cgtcagccct acttctctct attatgatgt caacgttcag ggtacccgca atgtgctggc
    23641 ggccatggaa aaaaatggcg ttaaaaatat catctttacc agttccgttg ctgtttatgg
    23701 tttgaacaaa cacaaccctg acgaaaacca tccacacgac cctttcaacc actacggcaa
    23761 aagcaagtgg caggcggagg aagtgctgcg tgaatggtat aacaaagcac caacagaacg
    23821 ttcattaact atcatccgtc ctaccgttat cttcggtgaa cgcaaccgcg gtaacgtcta
    23881 taacttgctg aaacagatcg ctggcggcaa gtttatgatg gtgggcgcag ggactaacta
    23941 taagtccatg gcttatgttg gaaacattgt tgagtttatc aagtacaaac tgaagaatgt
    24001 tgccgcaggt tacgaggttt ataactacgt tgataagcca gacctgaaca tgaaccagtt
    24061 ggttgctgaa gttgaacaaa gcctgaacaa aaagatccct tctatgcact tgccttaccc
    24121 actaggaatg ctgggtggat attgctttga tatcctgagc aaaattacgg gcaaaaaata
    24181 cgctgtcagc tctgtgcgcg tgaaaaaatt ctgcgcaaca acacagtttg acgcaacgaa
    24241 agtgcattct tcaggttttg tggcaccgta tacgctgtcg caaggtctgg atcgaactct
    24301 gcagtatgaa ttcgtccatg ccaaaaaaga cgacataacg tttgtttctg agtaatgcag
    24361 acgagtcaga gaaaaaagga ccaggttgga ctacgattat tcggaaagag gtactgatta
    24421 aattatcagt atgtggattt ccgagtaatc cgagtaaccc agatgctgat ggtgtgtagt
    24481 gttatcgtaa ggtttaatca aagttatgcg gaactaagct ttggtatata gccataacgt
    24541 ttggctaacc tatggcaata atttacgcac tatcagccac ttagaggtag gaaaagaatg
    24601 acgaatttaa aagcagttat accggtagca ggtcttggga tgcatatgtt gcctgccact
    24661 aaggcgattc ccaaagagat gctaccgatc gtcgacaagc caatgattca gtacattgtt
    24721 gacgagattg tggctgcagg gatcaaagaa atcctcctgg taactcacgc gtccaagaac
    24781 gcagtcgaaa accacttcga cacctcttat gaattagaat ctctccttga acagcgcgtg
    24841 aagcgtcaac tactggcgga agtacaatct atctgtccgc cgggcgtgac cattatgaac
    24901 gtgcgtcagg gtgaaccttt aggtttaggc cactccattt tatgtgcgcg acctgccatt
    24961 ggtgacaatc catttgtcgt ggtgctgcca gacgttgtga tcgacgacgc cagcgccgac
    25021 ccgctacgct acaaccttgc tgccatgatt gcgcgcttca atgaaacggg acgcagccag
    25081 gtgctggcaa aacgtatgcc gggcgacctc tctgaatact ccgtcatcca gaccaaagaa
    25141 ccgctggatc gtgaaggcaa agtcagccgc attgttgaat ttatcgaaaa gccggatcag
    25201 ccgcagacgc tggactcaga catcatggcc gttggtcgat atgtgctttc tgccgatatt
    25261 tggccggaac tggaacgtac tcagcctggt gcatggggac gtattcagct gactgatgcc
    25321 attgctgagc tggcaaaaaa acaatccgtt gatgcaatgc tgatgactgg cgacagttac
    25381 gactgcggta aaaaaatggg ctatatgcag gcgtttgtga agtatggact acgcaacttg
    25441 aaagaagggg cgaagttccg caaaggtatc gagaagctgt taagcgaata atgaaaatct
    25501 gaccgaatgt aacggttgat aaggaaataa tatcggcagt gaaaaactat agatcaataa
    25561 atatcactgc tgttgagctt ttataattaa ctatgctacc aatgaataaa tagtgtgcag
    25621 atatgtattt tttgtttggt agtcttgttt ctccctctga ataataagat aataaacgct
    25681 tggagatgat ttttttaaca tggattatca tttcgtaca
//
LOCUS       DAZFTN010000067        25286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFTN010000067 DAZFTN010000000
VERSION     DAZFTN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..1693
                     /gene="lsrA"
                     /locus_tag="ACHKV2_003044"
     CDS             158..1693
                     /gene="lsrA"
                     /locus_tag="ACHKV2_003044"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO6940889.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            1687..2715
                     /gene="lsrC"
                     /locus_tag="ACHKV2_003045"
     CDS             1687..2715
                     /gene="lsrC"
                     /locus_tag="ACHKV2_003045"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO6940890.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            2715..3707
                     /gene="lsrD"
                     /locus_tag="ACHKV2_003046"
     CDS             2715..3707
                     /gene="lsrD"
                     /locus_tag="ACHKV2_003046"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO6940891.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            3719..4741
                     /gene="lsrB"
                     /locus_tag="ACHKV2_003047"
     CDS             3719..4741
                     /gene="lsrB"
                     /locus_tag="ACHKV2_003047"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO6940892.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            4768..5643
                     /gene="lsrF"
                     /locus_tag="ACHKV2_003048"
     CDS             4768..5643
                     /gene="lsrF"
                     /locus_tag="ACHKV2_003048"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO6940893.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            5667..5957
                     /gene="lsrG"
                     /locus_tag="ACHKV2_003049"
     CDS             5667..5957
                     /gene="lsrG"
                     /locus_tag="ACHKV2_003049"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO6940894.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            6014..6772
                     /gene="tam"
                     /locus_tag="ACHKV2_003050"
     CDS             6014..6772
                     /gene="tam"
                     /locus_tag="ACHKV2_003050"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO6940895.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(6776..7690)
                     /gene="yneE"
                     /locus_tag="ACHKV2_003051"
     CDS             complement(6776..7690)
                     /gene="yneE"
                     /locus_tag="ACHKV2_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO6940896.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(7887..9338)
                     /gene="uxaB"
                     /locus_tag="ACHKV2_003052"
     CDS             complement(7887..9338)
                     /gene="uxaB"
                     /locus_tag="ACHKV2_003052"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO6940897.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(9565..10983)
                     /locus_tag="ACHKV2_003053"
     CDS             complement(9565..10983)
                     /locus_tag="ACHKV2_003053"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6940898.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSISIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(11122..11481)
                     /gene="yneG"
                     /locus_tag="ACHKV2_003054"
     CDS             complement(11122..11481)
                     /gene="yneG"
                     /locus_tag="ACHKV2_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO6940899.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(11481..12407)
                     /gene="glsB"
                     /locus_tag="ACHKV2_003055"
     CDS             complement(11481..12407)
                     /gene="glsB"
                     /locus_tag="ACHKV2_003055"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO6940900.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(12471..13859)
                     /gene="sad"
                     /locus_tag="ACHKV2_003056"
     CDS             complement(12471..13859)
                     /gene="sad"
                     /locus_tag="ACHKV2_003056"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO6940901.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            13960..14841
                     /gene="yneJ"
                     /locus_tag="ACHKV2_003057"
     CDS             13960..14841
                     /gene="yneJ"
                     /locus_tag="ACHKV2_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6940902.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            14919..16034
                     /gene="yneK"
                     /locus_tag="ACHKV2_003058"
                     /pseudo
     CDS             14919..16034
                     /gene="yneK"
                     /locus_tag="ACHKV2_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            16185..17375
                     /gene="ydeA"
                     /locus_tag="ACHKV2_003059"
     CDS             16185..17375
                     /gene="ydeA"
                     /locus_tag="ACHKV2_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO6940903.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(17400..18065)
                     /gene="marC"
                     /locus_tag="ACHKV2_003060"
     CDS             complement(17400..18065)
                     /gene="marC"
                     /locus_tag="ACHKV2_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO6940904.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            18277..18711
                     /gene="marR"
                     /locus_tag="ACHKV2_003061"
     CDS             18277..18711
                     /gene="marR"
                     /locus_tag="ACHKV2_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO6940905.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            18731..19114
                     /gene="marA"
                     /locus_tag="ACHKV2_003062"
     CDS             18731..19114
                     /gene="marA"
                     /locus_tag="ACHKV2_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO6940906.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            19146..19364
                     /gene="marB"
                     /locus_tag="ACHKV2_003063"
     CDS             19146..19364
                     /gene="marB"
                     /locus_tag="ACHKV2_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO6940907.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(19395..20294)
                     /gene="eamA"
                     /locus_tag="ACHKV2_003064"
     CDS             complement(19395..20294)
                     /gene="eamA"
                     /locus_tag="ACHKV2_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO6940908.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            20489..21676
                     /gene="ydeE"
                     /locus_tag="ACHKV2_003065"
     CDS             20489..21676
                     /gene="ydeE"
                     /locus_tag="ACHKV2_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO6940909.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(21717..22112)
                     /locus_tag="ACHKV2_003066"
     CDS             complement(21717..22112)
                     /locus_tag="ACHKV2_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6940910.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            22207..23178
                     /gene="ftrA"
                     /locus_tag="ACHKV2_003067"
     CDS             22207..23178
                     /gene="ftrA"
                     /locus_tag="ACHKV2_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFO6940911.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            23286..23381
                     /gene="mgtS"
                     /locus_tag="ACHKV2_003068"
     CDS             23286..23381
                     /gene="mgtS"
                     /locus_tag="ACHKV2_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO6940912.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            23381..23485
                     /gene="mgtT"
                     /locus_tag="ACHKV2_003069"
     CDS             23381..23485
                     /gene="mgtT"
                     /locus_tag="ACHKV2_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO6940913.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(23600..24490)
                     /gene="dgcZ"
                     /locus_tag="ACHKV2_003070"
     CDS             complement(23600..24490)
                     /gene="dgcZ"
                     /locus_tag="ACHKV2_003070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6940914.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(24745..25137)
                     /gene="ydeI"
                     /locus_tag="ACHKV2_003071"
     CDS             complement(24745..25137)
                     /gene="ydeI"
                     /locus_tag="ACHKV2_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO6940915.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         6452 a         6306 c         6091 g         6437 t
ORIGIN      
        1 tctacgagca tgaacaaacg caaccgtgaa aatcaaaata gtataaattg tgatctattc
       61 gtcggaaata tgtgcaatgt ccacctaagg ttatgaacaa attaaaagca gaaatacatt
      121 tgttcaaaac tcacctgcaa aactgaacgg gggaaatatg caaacgagtg atacccgcgc
      181 gttaccgcta ctttgcgccc gctcggttta taaacagtat tcaggggtca atgtcctgaa
      241 aggcatcgat tttacgttgc atcaggggga ggtccacgcc ctgctcggcg gcaatggtgc
      301 cggtaaatcg acgttaatga agattattgc cggtattacc tctgctgata gcggtacgct
      361 ggagattggg ggcaacaact acgccagatt aacgccagtt catgctcatc agctgggtat
      421 ttatctcgtt ccccaggaac cgctgctttt cccaagcctg tcgataaaag aaaacatcct
      481 gtttgggctg gcaaaaaaac agctctccat gcagaaaatg aagaacttgc tggcggcgct
      541 gggctgccag tttgatctgc atagtctggc aggatcgctg gatgtcgccg atcgccaaat
      601 ggtggaaatc ctccgcgggc tgatgcgcga ctcgcggatt ctgatcctcg atgaacctac
      661 cgcctcgctt acccctgcgg aaaccgaacg cttgtttact cgcttgcaag agctgcttgc
      721 tactggcgtg ggtattgttt ttatctcgca taagctgccg gaaattcgcc agattgccga
      781 tcggattagc gtgatgcgcg acggaaccat cgccttaagc ggcaaaacca gcgaactgtc
      841 taccgacgac attattcagg ccatcacccc agcggtacgg gaaaaatcgc tctctgccag
      901 ccaaaaatta tggctggagt tacctggcaa ccgcccacaa catgccgcag gaacgtcggt
      961 gctgacactg gaaaatctga ctggcgaagg tttcaggaat gtcagcctga cgctaaatgc
     1021 cggagaaatt ctgggtctgg ctgggctggt gggtgccgga cgcacagaac tggccgagac
     1081 gctctatggt ctacgtactt tgcgtggcgg acgcattatg ctgaatgata aagagatcaa
     1141 tagattatcc actggagaac gtttactgcg cggtctggtt tatctgccgg aagatcgcca
     1201 gtcatccgga ctgaatctcg atgcttcgct ggcctggaac gtctgcgccc ttactcataa
     1261 ccttcgtgga ttctgggcga aaaccgcgaa agataatgcc accctggaac tttatcgtcg
     1321 ggcgctgaat attaaatata accaaccgga acaagctgca cggacattat ccggtggtaa
     1381 ccagcaaaaa atcctgattg ccaaatgcct ggaagcctcg ccgcaagtat tgattgtcga
     1441 tgagccgacg cgcggcgtgg atgtctcggc gcgtaatgat atctaccaac tgttgcgcag
     1501 catcgccgca cagaatgtgg ctgtgctgct tatctcctct gatctggaag agatcgaact
     1561 gatggcagat cgtgtgtatg tgatgcatca gggcgaaatt acccactctg cactgaccgg
     1621 gcgcgatatt aatgtcgaga ctattatgcg cgttgccttc ggcgatagtc agcgtcagga
     1681 ggcggcatgc tgaagtttat tcagaacaac cgtgaaatca cggcactgct ggcggtgctg
     1741 ctgctgtttg tattacccgg ttttctcgac cgccagtatt taagtgtgca aacgctgacc
     1801 atggtttata gcagcgcgca aatcctgatc ctgctggcaa tgggcgcgac gctggtaatg
     1861 cttacgcgca atattgatgt ttcagtgggt tcgattaccg gaatgtgcgc ggtgctgttg
     1921 gggatgttac tgaacgcagg atattcacta cctgttgcct gtgtcgcgac tttactgctt
     1981 ggtttgctcg cgggattttt caacggtgtc ctggtcgcgt ggctaaagat ccctgccatt
     2041 gttgccaccc ttggcacgtt agggttgtac agaggcatca tgttgctgtg gactggcggt
     2101 aaatggattg aagggttacc cgccgaactg aaacagctct ccgccccgct gctgcttggc
     2161 gtttcagcaa ttggttggtt aacgataatt ctggtggcat ttatggcctg gctgctggcg
     2221 aagacagcgt ttggacgcag tttttatgcc acgggcgata atttacaggg cgctcgtcaa
     2281 ctgggcgttc gtacggaagc cattcgcatt gtggcatttt cgttgaacgg ctgcatggcg
     2341 gcactggcgg gaattgtgtt tgcttcgcag attggtttta tccccaacca gaccggtacc
     2401 gggctggaga tgaaagcaat tgcagcctgc gtgctgggcg gcattagttt gctcggcggt
     2461 tccggtgcga tcatcggtgc ggtactcggc gcatggttcc tgacgcagat cgatagcgta
     2521 ctggtgctgt tgcgcattcc ggcatggtgg aatgatttta tcgcgggtct ggttctgctg
     2581 gcggtgctgg tgtttgatgg acgcctgcgt tgtgcgctgg aacttaatct gcggcggcaa
     2641 aaatatgccc gctttatgac gccaccgcca tccgttaaac ccgcttcgtc aggtaaaaaa
     2701 cgggaggctg cataatgcgt attcgctacg gttgggaact ggctcttgcc gcactgcttg
     2761 ttattgagat tgtctcattt ggtgcaatta acccgcggat gttagatctc aatatgttgc
     2821 tgttcagcac cagtgacttt atctgcattg gcattgtcgc cctaccgctg acgatggtga
     2881 ttgtcagtgg cgggatcgat atttcgtttg gttcgaccat cggcctctgc gccattgcat
     2941 tgggcgtact gtttcaaagt ggtgtgccga tgccgctggc gattctcctg accttactgc
     3001 tcggcgcatt gtgcgggctg atcaacgccg gattaattat ctataccaaa gttaacccgc
     3061 tggtgattac gcttggcacg ctgtatctgt ttgccggaag cgccctgctg ctttccggga
     3121 tggccggggc gacggggtac gaaggtattg gtggattccc gatggcgttt acagatttcg
     3181 ctaacctgga tgtgttgggg ctccccgttc cgctgattat cttcctgata tgtctcctcg
     3241 ttttctggct ctggctgcat aaaacccatg ccggacgtaa cgtgtttttg attgggcaaa
     3301 gcccgcgcgt ggcgctttat agcgcgattc cagttaaccg taccttatgt gcgctctatg
     3361 ccatgacggg gctggcgtct gcggtcgccg ctgtgctgct ggtgtcgtat tttggttcag
     3421 cacgttccga tctcggtgcg tcgtttctga tgcccgccat caccgccgtg gtgcttggcg
     3481 gtgccaatat ttatggtggt tccggtgcca ttatcggcac cgccattgcg gttttattag
     3541 tgggatattt gcaacaaggt ttgcaaatgg caggagtgcc aaatcaggtg tccagcgccc
     3601 tttccggtgc gctacttatc gtcgttgtcg taggtcgttc cgttagcctg catcgccagc
     3661 aaattaaaga gtggctggcg cgtcgggcca ataacccatt gccataaagg atatcttcat
     3721 gacacttcat cgctttaaga aaatcgcctt acttagcgct cttggcattg ccgcaatctc
     3781 tatgaatgtg caggccgcag agcgtattgc atttattccc aaactggttg gcgtgggatt
     3841 ttttaccagc ggtggcaacg gcgcacaaca agcgggtaaa gagctgggcg ttgatgtgac
     3901 ctacgacggg ccgacagaac ccagtgtttc tggtcaggta cagttgatta ataacttcgt
     3961 caatcaaggt tataacgcca ttatcgtttc tgcggtttcg cctgatggct tgtgtccggc
     4021 actgaaacgc gccatgcaac gtggtgtgag agtgctgacc tgggactctg atactaaacc
     4081 ggagtgccgc tcttactaca ttaatcaggg aactcccgcc cagttgggag gtatgttggt
     4141 ggatatggcg gcgcgtcagg tgaataaaga caaagccaaa gtcgcgtttt tctactcaag
     4201 ccccaccgtt acggaccaaa accagtgggt gaaagaagcg aaagcgaaaa tcgccaaaga
     4261 gcatcctggc tgggaaattg tcactacgca gtttggctat aacgatgcca ctaaatcatt
     4321 acaaaccgca gaaggaatat taaaagcgta tagcgatctc gacgccatta tcgcccccga
     4381 tgccaatgcc ctgcccgctg ccgcacaagc cgcagaaaac ctgaaaaatg acaaagttgc
     4441 gattgtcgga ttcagtacgc caaacgtgat gcgtccatat gtggaacgcg gcacggtgaa
     4501 agaatttggc ctgtgggatg tggttcagca aggcaaaatt tcagtttatg tcgcggatgc
     4561 attattgaaa aaaggatcaa tgaaaacggg cgacaagctg gatattcagg gcgtaggtca
     4621 ggtggaagtc tcgccaaaca gcgtccaggg ctatgactac gaagcggatg gtaatggcat
     4681 cgtactgtta ccggagcgcg tgatattcaa caaagagaat atcggcaaat acgatttctg
     4741 atgtgcatta cttaaccgga gtaagttatg gcagatttag acgatattaa agatggtaaa
     4801 gattttcgta ccgatcaacc gcaaaaaaat atccctttta ccctgaaagg ttgcggtgcg
     4861 ctggattggg gaatgcagtc acgcttatcg cggatattta atccgaaaac gggtaaaacc
     4921 gtgatgctgg cttttgacca tggttatttt cagggaccaa ctaccggact tgaacgcatt
     4981 gatataaata tcgccccgct gtttgaacat gccgatgtat taatgtgtac gcgcgggatt
     5041 ttgcgcagcg tagttccccc ggcgaccaat aagccggtgg tactgcgggc gtcaggtgcg
     5101 aactctatcc tggcggaatt aagcaatgaa gccgtggcgt tatcgatgga tgacgccgtg
     5161 cgcctgaaca gttgcgcggt ggcggcgcag gtttatatcg gcagcgaata tgaacatcag
     5221 tcgatcaaaa atattattca gctggtcgat gccggaatga aagtgggaat gccgaccatg
     5281 gccgtgactg gcgtgggcaa agacatggtg cgcgatcagc gttatttctc gctcgcgact
     5341 cgaatcgccg ctgaaatggg gacgcaaatt atcaaaacct attatgtcga aaaaggtttt
     5401 gaacggattg ttgccggatg tccggtaccc attgttattg ctggcggtaa aaaattaccg
     5461 gagcgcgagg cgctggaaat gtgctggcag gctatcgatc agggcgcttc tggtgtggat
     5521 atggggcgta atattttcca gtctgaccat ccggtggcga tgatgaaagc cgtacaggcg
     5581 gtggttcacc ataacgaaac ggctgatcgg gcatatgaac tctatctgag tgaaaaacag
     5641 taactgcgga tttaaggaga agaattatgc acgtcacact ggttgaaatt aacgttcatg
     5701 aagacaaggt tgacgagttt atcgaagttt ttcgccagaa ccacctgggc tctgtacagg
     5761 aagaaggcaa tttgcgcttc gatgtcttac aggacccgga agtgaattcg cgcttttata
     5821 tctacgaagc ctataaagat gaagacgcag tggcgttcca taaaaccacg ccccactaca
     5881 aaacctgtgt cgcgaaactg gaatctttaa tgaccgggcc gcgtaaaaaa cgtctgttca
     5941 atgggttgat gccgtgaggc gaatttatca gttttatcta caattggggt aacgcgctga
     6001 cgggagtaaa taaatgtctg actggaaccc ctctttatat ctgcactttg ccgctgaacg
     6061 atcgcgtccg gcggtggagc tgcttgccag agtgccgctg gagaatgtcg attatgttgc
     6121 cgatcttggc tgtggcccag gtaacagcac cgccctgctg catcaacgtt ggcctgctgc
     6181 caggataacg ggcatcgact cgtctccggc gatgattgct gaagcgcgca gtgctttgcc
     6241 agactgccag ttcgtggaag cagacatccg caactggcaa ccggaacagg cactcgatct
     6301 gatttttgct aatgcctcac tgcaatggct gcccgaccac tacgaattgt ttcctcactt
     6361 ggtttcttta cttaatccgc agggcgtgct ggcagtacag atgccagata actggctgga
     6421 gccgacccat gtgctcatgc gcgaagttgc ctgggaacaa aactacccgg atcgcgggcg
     6481 tgagtcgttg gctggtgttc atgcttacta cgatattttg agcgaagccg gatgtgaggt
     6541 cgatatctgg cgaaccacct actatcacca gatgccgtcg caccaggcga ttatcgattg
     6601 ggtgaccgcc actggattac gtccgtggtt acaggatctg accgagagcg aacagcagct
     6661 ttttcttacg cgctaccatc agatgctgaa agagcagtat ccactgcaag agaacggaca
     6721 gatactgctg gcatttccgc gtctgtttat tgttgcccgc cgtacggagt aaatatcacg
     6781 tcagttggta atggcgatcg ggaagaactt ttgctggaat ttcggcttca tcgttcatct
     6841 gtaagaggtc aatttcaata gcgttacaga tggcatccag cggtaaatca ttgttttcag
     6901 taccgaacgg atcttccagt tcttccgcca gacaatccag cgaaataaaa gtataggaaa
     6961 tcagcacaga gataaaaggc gtcatgtaat gcaggtccac gaccagcgcg aacggcagca
     7021 tgatacaaaa cagataaacg gtacgatgca aaatcagggt gtaggcaaag ggaattggcg
     7081 tgtaggcaat gcgctcgcag cctgccagaa ccgctgaaat atcgttaagc cgatcgttga
     7141 ggctaataaa caggatatct gaaagttgtc cattgcgtcg ctgaaccgcc aaccactccc
     7201 ccattattaa caagatgcgg ttagctggag agttcgaagc cagtacacgc tgaagatctt
     7261 cagtcttgag ataatgagcc agcacttcca cctgtggctg tttgcgtaat gtcatgcgta
     7321 aacagtgggc gaaggcgatt tgcagccggg caaactccct tacacttgcc gaatccggca
     7381 atgtcgtttt tacctcgcgc agtaacgacc gtgaggcaat catcaactgc ccccaaagtt
     7441 tgcgcgcttc aacgtaacgg gcgtaaccgg cattattacg aaaaccaaga aaaatggcga
     7501 tggcgacacc gagaatgctg aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc
     7561 agggcagcat gaaaataaca gcgatagaaa agagaaaatt gagtagtaag cgcgaggata
     7621 tcttggataa tactgagcca tgccagacaa aaatacggcg cagccagtgt tgttgtggac
     7681 gaacaatcat ggttatcttc aggcgtggaa aagtcgccct attaaacgtg attacgatca
     7741 cattctcaag acactactta caaattacct acaaaattac aacattaaca ggtgctgcat
     7801 gtggcacctg ttaatgataa aggcttatag gatgttgtaa ctaatataat gaaataagaa
     7861 acccggtcga aaccgggttc agagtattag cacaacggac gtacagcttc gcgcatccct
     7921 ttttcgagaa tcgcatccag gtcattagcc acctgctcga ccagaccagg cacttgcgtc
     7981 aggtcctgct cccagtggtc tttttctgcc aacacaatcg ctaccagttc ctgggtaccg
     8041 ataacacggt cacgatgctg actccagagt tgctgataac gttccagcca gtgtgcatcg
     8101 tcctgtaccg gatatgtttc tccattacgc tcaccgcgat agaacgcaat caatgccgct
     8161 aatgcgaaag taaggcgcgc cggaagtgtg ccgtttgcct tctgccctgc cagcagctgc
     8221 ggcaggatgc gggtacggaa cttggtcata ccgttgagcg cgatagacag cagctgatgc
     8281 ttaatgtacg ggttacggaa acgcccggtg actgcactgg cgaaagattc cagttcatcg
     8341 cgtggcaaat ccagtaccgg aataatttct tcgtagatgg ctttttcaac gaatgcgcaa
     8401 atttcagcat cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa cgccaccggc
     8461 accaacgcgg tgtgtgcacc gttgagtatt gccactttgc gttctttata cggtttaata
     8521 tcgtcaacaa tcagcacgtt gagcggatat ttgtccagac gcagctcggt cgctaaggat
     8581 ttcggtccct gaatcacaaa caagtaaaag tgttcagcgg tgtcgaggaa accatcgtga
     8641 taacccagtt cttcttccag tttagccact tcatcacgcg gataaccggt aacgatacgg
     8701 tccaccagcg tagaacagaa gctgttagcc tgatccagcc attgaataaa tgcttctggc
     8761 agtgcccact cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc attgtagtcg
     8821 atcaactcac acgggatgat gatccaacct ttatccagcg caccgttgaa atggctaaaa
     8881 cgttcgaaca gcagacgggt cagttttgcc ggataactta ccgctggcgc gtcatcgaat
     8941 ttatcgcccg catggtagct gatacccgct tcggtggtgt tagagaaaac aaagcgcatt
     9001 tccgggttgt gcgccagttt caggaattca tcgtattcac tgtagacgct gatttcacga
     9061 ttaacagagc gaatcagacg cgcgtcgctg accgcttccc ccttctcatt caggccacgg
     9121 ataatggtgg tgtacagacc atcctgcgtg ctcagtgacg gcgggaatga agtttcaatc
     9181 ggacgaacaa cgaccacacc agaattcaga tcggtgtgct cattcaggag atctatttgc
     9241 cagtcaacaa aggcgcgcag gaagttacct tcaccaaact gaatgatacg ttctggatac
     9301 tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa tgggttccct tctgattagt
     9361 catacaacct gtttgaattg gtacgacagg ttagcaaact ttaatacgac gaacccctgt
     9421 tttgatcaac tcctgatgat taatgagcag ttttatgaga aaagtgtggc gcggatcatg
     9481 gtttaatcga ggaaatacgc cttttcctgg atcataaagt ggtagaacac attgcattca
     9541 aatcgcgcgg aatgaataaa gatgtcagac aacttcctca ccgtaacgca tagtgctggt
     9601 acggttgcgc ccatctttct tcgagcgata cagacaggta tcggcttcga ccatcaattt
     9661 attaaagtca tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc caatacttac
     9721 cgtgagataa agcgtttttt gttgccaggt gaatggttgc agttcaacgc ctttacggat
     9781 tttttccgcc attagcagac catcgacagg attcactgac ggcactgcaa cagcaaattc
     9841 ttcgccgccc atgcgcgcca ccagcccctt atcaccgaca atcttctgaa tatgccgggc
     9901 aaacacgctt aacactttat cgccacattc atgtccatag ttatcgttaa tacttttgaa
     9961 atagtcgata tcaagtagca tgacagtcag atgttgtgtc tgtttcagcg atggactttt
    10021 cagcgcctca taaagaccgg accgtgagta aacctgagtc agaaaatcaa agtcggctcg
    10081 cagcgcaact tgcttcatta gcgaattgat cgctgccacg ctaaaagaaa ccataattgg
    10141 gcatatcgcc atcgtggcaa taccgagacg tgcggagaac atttgcggaa tggagaacgg
    10201 cgaaccgacc gagatatcaa tcaccgaatt agccaccagt acgatttcca ccgcaccggt
    10261 gacaaaggtc aacagacatg ttacctgcgg cgtatagcgc actgcacacc agattaatgc
    10321 aggcagcggg aacgccagac tccccgctcc gccaatgact accgaggcaa taaccgacac
    10381 aataagcgcg atggcgggca tcatctgttc tgctttaaag cgcggcagca ctccaggaat
    10441 agccaacgtc agcatacaag gcacgatcaa cacgcccgtt gagaattgct cactgaacca
    10501 gtccgcaagc aaaggccaga aatccagact atcaatactg accgaaccaa tcgccccgac
    10561 aatagcgcat aataatgcgg caatcagaca gtaattaaat agccgtaagg cacttactgg
    10621 ctcatattta tttttcccca aacgcttatc ccgcacgacc agtaaggcga cggtaacaat
    10681 aaacatcata ttggagaaat tgatagccag tgaaaccagt ccccattcgg tggttatggc
    10741 atcataaacc agcatcgcca cataactgat cgcatagtaa tgcagtcgat taagccagac
    10801 atagcgggca aacacccccg ccattacgcc attcagcggc cagaaaagag aaagttcgtg
    10861 aaccagccgt aactctgcac caataaaata gaacagcgtg gttagcgcaa aaatagagat
    10921 gctattacgt agcagatgac cttcctggaa cacacggaaa gttgaaatgg ggtgtacatg
    10981 cattaattat ctgtccatac aattgcgggt ggcaattatc tcaacgtaat aagtaaattc
    11041 tcataaagga tagcatgttc gctgacagcg gcggcaaata aaggtaccct gggaatgcta
    11101 ttttgtcgtt ttccgcaatt ctcatggctg attcatctgt acaaccagcc agtgatagat
    11161 taccgccacg atataacgtt gttgttcttc gcttagcgaa acaccatgcg ggatgttatg
    11221 ccacttagcc agacaaccgc gacagcaggt cgcggtggcg tgctgagcga taaacaccgg
    11281 atgcccgcgc atgggggttt gcttaccgtc attagccggt aatgccggag caagacgttt
    11341 agcgacaaaa tccgccgcat gttgttcgat gactggcgcg cctttctcca gacaatactg
    11401 acgctctttc atgcccaaac gaaagcgaga tcgaaatttt gaacgggata aacgcgcgaa
    11461 gagtggatcg agagactgca ttaataaacc gaacgcccta actgtttcgt caattgttca
    11521 aggacggcga tacccgcaag cgagttacct gcatcatcca gttccggact ccagactgcg
    11581 atggccattt catgcggaac aatcgccaca ataccgccac caacgccaga tttcgccggt
    11641 agtcccaccc gccaggcaaa ctcccccgcg ttctggtaca taccactggt cgccatcagc
    11701 gcgttaattt gccgtgcctg cattggcgtc accacaggtt catcaatatg aatagctttc
    11761 ccctgattag ccagaaagac aaacgtccgg gccagctcta cacagctcat tttcagagcg
    11821 cagtaatgaa aatagttttg cagaacggtt gtcacgtcat gatggaaatt gccaaacgac
    11881 ttcatcagcc aggcgatagc cgcatttcgc gcggaatgtt caaattcgga acgcgctacc
    11941 accgtatcgt aggaaatatc agacacaccg cttaagccgc gcacgacttc cagcatacgt
    12001 tgccgtggtg cgcttaatcg cccttgcaac atatcgcaga ccaccagcgc accagcatta
    12061 ataaacggat tacgcggaat accctgctcc atttccagtt gcactaaaga attgaacggt
    12121 gatccagacg gatctttgcc gacgcgttgc cagatttcct cttcggagta atgacgcatg
    12181 gcgacaacga gactcagcac tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct
    12241 ccggcctgaa aaagctgtcc gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct
    12301 actgtagcca gcgccggaat ataatccgcg actttaccct gaccaatgag cggccgcact
    12361 tgccgcaaga tgttttctaa aattgcatta tccatggcga ctgccacttt ctactcctgg
    12421 accgcaggtc tgaaaagacc tgcgagtata tcagagctga atatgtcgcg tcagatccgg
    12481 tctttccaca ccgtctggat attacagaat tcgtgtaagc cgaaatggga aagctcacga
    12541 ccaaagccac tctttttcac gccaccaaag gccactcgcg cgtcgctggc acaataacca
    12601 ttgataaaca ccccaccgca ttccagacgt gccgccatct gtctggcctg tgtttcgtcg
    12661 gtggtaaaaa tggtcgctga aaggccgaac tcactatcat tagccagttc cagcgcatgt
    12721 tctgcatctt tcgcaatggt gattgccgca acggggccaa acatttcttc ccgaaaagcg
    12781 gtcatttctg gggtaacatt cgccagaacc gttggcggat agtagttacc tgccccagcc
    12841 atcttttccc cgcccagtaa caaacgcgca ccctgcgcca gggttttctc cacctgatga
    12901 tgcagctcat cacgtaaatc gaaacgagcc attggtccga gagcgttctc ttcgtcacgg
    12961 ggatcgtcca ttttcaaggc tgccgcagct gccacaaaac gttcggtaaa tgccgaagca
    13021 attccctctt cgataataaa gcgttttgcc gctgcacaaa cctgtccggt attctgataa
    13081 cgtccggcta ccgccgcttt aactgccagt tccagatcgg catcgttaag cacaataaat
    13141 ggatccgaac cgcccagttc cagtacgcat tttttcagtg ccgctccagc ctgtgcacca
    13201 atagccgctc cagcacgaac acttccggtc accgtgatag cagcaatgcg cgagtcttta
    13261 atcatctgag tgacaccgtc attgtcggca ttcagccagc catatacgcc ttgtgggatt
    13321 cccgcatctt taaacacctg ggcaatgagc tgagcacagc ccatcacatt cggcgcatgt
    13381 ttaagtaagt atccgttacc agcaagaatg atgggaacag cgccacgcat cacctgccat
    13441 aacggaaaat tccacggcat aatcgccaga atcgtcccca acggtcgata ctcaataact
    13501 gcctgctgat tttccaccag cgtaggttcc gccttcagca ttgccggacc atgttctgca
    13561 taccagtcac acaaattcgc cgatttcgcc acttcagcgc gcgcctggtt gattggtttg
    13621 cccatttcgc gggtgatcat ttgcgccatt tcttcgctac gggcgcgcag agccttaccg
    13681 atatcacgca gtttttcagc acgataatct atatttgtct cgcgccagtc acgaaagcct
    13741 gctgccgcca gctgaagtgc gttttcgata tcatcagcgc cagcccacgg caacacagaa
    13801 agttgttcac ccgtggcagg atttatcgaa attgcatgag ttgccggagt aatggtcatc
    13861 ggggtatctc ctttatgagt catggtatga agatacgcag atttactctt gctttaaaat
    13921 gaataatatt aagccactta ttcacgaatc gagaatatta tggatctgac ccaactggag
    13981 atgttcaacg cggttgccga agctggcagc attacgcagg ctgcagcaat agtgcatcgc
    14041 gtgccgtcga atctcactac ccgtctacgc cagctggaaa cagaactggg ggtagatctg
    14101 tttattcgcg agaatcagcg tttacgtcta tctcctgccg ggcataactt tttacgctat
    14161 agccaacaaa ttctcgcgtt agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg
    14221 caaggtttat tttctctggg ttcgctggaa agcaccgcag cagttcgcat tccggccacg
    14281 ctggcggaat tcaaccgtcg ttatcccaaa attcagtttt cactttccac cggcccttcc
    14341 ggcactatgc tggaaggtgt gctggaggga aaacttaatg cggcgtttat tgatggaccc
    14401 attaaccata ctgccattga cgggataccg gtataccgcg aggagctgat gatcgtcacg
    14461 ccacaaggtc atgcgccagt aatccgtgcc agtcaggtta atggcagtaa catttatgcc
    14521 ttccgcgcca attgttcgta tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc
    14581 gctccgggaa ctatccatga gatggagtct tatcacggaa tgttggcctg tgtggtcgca
    14641 ggagcaggta ttgcgcttat tccgcgctct atgctggaaa gtatgccggg gcatcaccag
    14701 gttgaagcgt ggccgttagc tgagcaatgg tgttggctaa caacctggct ggtctggcgt
    14761 cgtggtgcga aaacgcgtcc gctcgaggca tttattcaac tactggatgt gcctgactcg
    14821 acaaaacagg gttaccaatg agctattttt gatagttctt gcggctaata tgctctatat
    14881 agtgatgttc cgatgactta tgactatatg gggcaaatat ggttacgcca gtaagcatca
    14941 gcaattacat atctcttcct gatgattttc ccgttcgtaa tattgcaccg caggtaaaag
    15001 aagttttaaa ggattttatt gatgcactta gtacaataat atgtaatgaa gagtggcgca
    15061 cgtctttaaa catcaactca gccacaaaaa agatatttaa taatcttgac aacttatcat
    15121 atattcagag gacttctttt cggggtaacg acacgctata caatgaaaag gttcagttta
    15181 aactcactta tcccgccagg aatggaagac acaaagaaaa tattgaattt caggtagtaa
    15241 taaatttaag ccctatttat ttagataatt tccgccatga tggagaaatt aatatttttt
    15301 gcgctcccaa cccaaagcct gttactatgg ggcgcgtatt tcagaccggc gtcgagcgtg
    15361 tgctctttct gtttctgaat gattttattg aacaattccc aatgatcaac cctggcgttc
    15421 ccatcaaaag agcgcataca ccacatattg aacccctgcc tccggatcac cataccgctg
    15481 cagattactt acgccagttt gatttgcttg tcctgaattt tatctctcgc ggtaattttg
    15541 tcatactccc ccgattatgg aataactctg aggttcacag atggttcgtc aataaagatc
    15601 ctaacttgat caccgccatt ctcgacataa cggacagcga attaaaagag gatttattgc
    15661 aaagcctgat ggattcatta ggttctaaca aacatgtact accctaagtc tgcatctgtt
    15721 ttttatccct tttagcagaa caagaatctc ctcattttca ggacttgttt ttatttttcg
    15781 ccaatatgtt gttacactat caccaattta tgaatcccaa tgaaagtgat ttgaatgacg
    15841 tgttgatgcc agcatcatta agtgatgata aaattatcaa acatatggcg cgtaagaccc
    15901 tcaaactgtt tgtaaaaaat gaaacaccgc caaaagttac tcatgaagat ctggtgaaaa
    15961 acaggcctcg ctcccctgtc agaccgccta tacccgcaac ctccaaaacg ccagacattc
    16021 ctgatcgtca ttaaaccgtg atgtttacca tctctctgac gcgtgaaaga atcagcgtca
    16081 gagaaacgga aaacgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt
    16141 aagatcattt catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg
    16201 tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca
    16261 acaccaccga atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc
    16321 aaaccgctca ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat
    16381 tgccttttat gttaatgacc agccaggttg aacggcgcaa attactgatc tgcctgtttg
    16441 tggtgtttat tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga
    16501 tcagtcgcat tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtccctgg
    16561 cgatccgtat ggctccggcc gggaagcgag cacaggcatt gagtttaatt gccacaggta
    16621 cagcactggc aatggtatta ggattacctc tcgggcgcat tgtggggcag tatttcggtt
    16681 ggcgaatgac cttcttcgcg attggtatcg gggcgcttat tacccttttg tgcctgatta
    16741 agttacttcc cttactgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattat
    16801 tccgccgccc ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt
    16861 acacggcata cagctatatt gagccttttg tgcaaaacat tgcgggattc agcgccaact
    16921 ttgccacggc attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg
    16981 gtaaactggg taatcaatat gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg
    17041 tgtgcctggc actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta
    17101 ttttctgggg gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc
    17161 tggcaccaga tgccaccgac gtcgcgatgg cgctattctc cgggatattt aatattggaa
    17221 tcggggcggg tgcgttggta ggtaatcagg tgagtctgca ctggtcaatg tcgatgattg
    17281 gttatgtggg cacggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc
    17341 gctggccagt gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt
    17401 taatgattcg ttttaatgat ttccaggatg ccgttaataa taaactgtac acccatacat
    17461 accagcagga accccatcaa gcgggagatc gcttcaatgc cacccttgcc caccagccgc
    17521 ataattgcgc cagagctgcg tagacttccc cacaaaataa ccgccaccag gaaaaagatc
    17581 agcggcggcg caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac
    17641 gccgagctaa taatcatcgc aatggttccc ggaccggcgg tacttggcat tgccagtggt
    17701 acaaaagcaa tattggcgct gggttcatct tccagctctt ccgacttgct tttcgcctcc
    17761 ggtgaatcaa tcgctttctg ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt
    17821 aagccgcctg caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc
    17881 ccggcgtaat acgccaccat catgatggca aagacgtaca ccgaggccat caacgactga
    17941 cgattacgtt cggcaccgtt catgttgcct gccaggccaa gaaataacgc gacagttgtt
    18001 aatgggttag ctaacggcag taacaccacc agccccaggc caattgcttt aaacaaatct
    18061 aacattggtg gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac
    18121 aaggataaag tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat
    18181 ttagcaaaac gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt
    18241 atcccctgca actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc
    18301 aatgaaatta ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg
    18361 cttaacgagt atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct
    18421 atccgctgcg cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg
    18481 ggagcactga cccgtatgct ggatcgcctg gtctgtaaag gctgggtaga aaggttgccg
    18541 aacccgaatg ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgt
    18601 gaacaatgcc atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg
    18661 gcggacgaag tggcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag
    18721 aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca ttttggactg
    18781 gatcgaggac aacctggaat caccactgtc actggagaaa gtgtcagagc gttcgggtta
    18841 ctctaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata
    18901 catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat
    18961 actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa
    19021 aaattacttt gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg
    19081 ctttttacat ccattaaacc attacaacag ctagttgaaa acgtgacaac gtcactgagg
    19141 caattatgaa accgctttta tccgcaatag cagctgcgct tattctcttt tccgcgcagg
    19201 gcgttgcgga acaaacccag caaccgctcg ttacttcctg tggcgatgtg gtggttgttc
    19261 ccccatcgca ggaacaacca ccgttcgatt taaatcacat gggtacaggc agtgacaaat
    19321 cggatgcgct gggcgtgccc tattataacc aacaagccat gtagtatatt caggccccgg
    19381 catatcgggg cttgttaacc tcttaccttc accgctttac gccactgcaa accaaataca
    19441 ttgatataca gtccggccat aatgagcaca gcccctaaga attgcagacc tgttaagcgt
    19501 tcatccagca gtaacgctgc acttgccaga ccgactaccg gcaccagtaa tgataatggt
    19561 gcgacacgcc atgtttcata gcgtccaagc agcgtacccc aaatcccgta gccaactatc
    19621 gtcgctacaa atgccagata catcaacgcc agaatggtgg tcatgtcgat agtgaccaga
    19681 ctgtgaatca tttttccgga gccatcgaga atgagcgagg cgacaaaaaa cggaattatc
    19741 gggattaatg cgctccagac gaccagagac attaccgccg gacgggatga atgagacatg
    19801 atctttttgt tgaagatatt gccactcgcc caactaaatg ccgctgccag agtcaacata
    19861 aagccgagca tcgccacatg ctgaccgtta aagctatctt cgattaacac cagtacgcca
    19921 gaaatcgcta acgcaatccc cgccagttgc ttaccgtgca gccgttcgcc aaaagtaaac
    19981 gcgccaagca cgatagtaaa aaacgcctga gcctgtaaga ccagcgaggc cagtccagcc
    20041 ggcataccga agttaatggc gcagaagaga aaggcaaact gtgcaaaact gatggttaaa
    20101 ccatacccca gcagtaaatt cagcggtact tttggtcgtg ccacgaaaaa gattgccggg
    20161 aaggcgacca gcataaagcg caaaccggcc agcatcagcg gtggcatgtt atgaagcccc
    20221 actttgatga ccacaaaatt aagcccccat acaacgacga ctagtagcgc caacacccca
    20281 tcttttcgcg acattctacc gcctctgaat ttcatctttt gtaagcaatc aacttagctg
    20341 aatttacttt tctttaacag ttgattcgtt agtcgccggt tacgacggca ttaatgcgca
    20401 aataagtcgc tatacttcgg atttttgcca tgctatttct ttacatctct aaaacaaaac
    20461 ataacgaaac gcactgccgg acagacaaat gaacttatcc ctacgacgct ctaccagcgc
    20521 ccttcttgcc tcgtcgttgt tattaaccat cggacgcggc gccacgctgc catttatgac
    20581 catttacttg agtcgccagt acagcctgag tgtcgatcta atcggttatg cgatgacaat
    20641 tgcgctcact attggcgtcg tttttagcct cggttttggt atcctggcgg ataagttcga
    20701 caagaaacgc tatatgttac tggcaattac cgccttcgcc agcggtttta ttgccattcc
    20761 tttagtgaat aacgtgacgc tggtggtgct cttttttgcc ctcattaact gcgcctattc
    20821 cgtttttgct accgtgctga aagcctggtt tgccgacaat ctttcatcca ccagcaaaac
    20881 gaagatcttc tcgatcaact acaccatgct caatattggc tggaccgtcg gcccgccact
    20941 cggcacgctg ttggtcatgc agagcatcaa tctgcccttt tggctggccg ctatctgctc
    21001 tgcgtttccc atgtttttca ttcaaatttg ggtaaagcgc agcgagaaaa tcatcgccac
    21061 ggaaacaggc agtgtctggt cgccgaaagt tttattacaa gataaggcac tgttgtggtt
    21121 tacctgctct ggctttctgg cttcttttgt tagtggcgca tttgcttcat gcatttcaca
    21181 atatgtgatg gtgattgctg atggtgattt tgccgaaaag gtggtcgcgg ttgttcttcc
    21241 agtgaatgct gccatggtgg ttacgttgca atattccgtg ggccgccgcc ttaacccggc
    21301 taacatccgc gcgctgatga cagcaggcac cctctgtttc gtcatcggtc tgatcggttt
    21361 tattttttcc ggcaacagcc tgctattgtg gggtatgtca gctgcggtat ttactgtcgg
    21421 tgaaataatt tatgcgccgg gcgagtatat gttgattgac catattgcgc cgccaggaat
    21481 gaaagccagc tatttttccg cccagtcatt aggctggttg ggcgcagcgg taaatccatt
    21541 agtgagtggt gttgtactta ccactctccc gtcgtcttcg ctgtttatca ttctggcgtt
    21601 agtcatcatt gccgcgtggg cgctgatgtt aaaaggtatt aacgccagac cccagaggca
    21661 accagcactc tgttgacgtt cgtgccgttc cactactggc acggcttcgc taatcattat
    21721 tctgaggcaa aggcatagcc ttcatcttcc cagccggtta tcccgccgag catgatttta
    21781 accggctttc cgagtcgagc cagtttgagt gccgcacggt ctgcaccgtt acaatgtggc
    21841 cccgcgcaat acaccacaaa tagcgtatcg tcgggccatt cggccattct ttctgcggtc
    21901 atttgcttat ggggtaaatg gagagcgcct gggatatgcc gacgcgcgaa gtcctcttca
    21961 cgtccaacca catgaagtaa aacgaaatca tcgccaatat aagccagaga gtcatgaaca
    22021 tcggcgcaat ctgtttcgac actcaagcgc gcgagaaaat gcgcctcagc aacttctggc
    22081 ttcgctgtgg taaattcagt gacataacgc atattgcttt gtccttttgg tttgttgtgt
    22141 taacaataat gtaactaatc tacctttcac tgaccgcctc tcgatatgcc agaaatgagc
    22201 aaaatgatga caacgttaca acgtccgctg gtcattgcac tggcatatga cgggctatgt
    22261 acttttgaat ttggcgttgc cgttgaaata ttcggcctgc cacgaccgga actgggcgat
    22321 aactggtatc gttttgctgt tgctgcggta gaaccaggtg cgctacaagc caccgggggg
    22381 atccacgtgg ttgctgatgg tggactggag ttattgccca acgccgatat gattattatt
    22441 cctggctggc gcggaataga tgccaaagtg ccggagatgc tatgccagtc attgcgcgag
    22501 gcacaccagc gcggtactcg cctggtatca atatgctctg gcgtgtttgt ccttgctgct
    22561 gcgggccttc ttcatcagcg aaaagcaacc acccactggc gctacactga acaactccag
    22621 caccgttacc cttcaatcag cgtaagtgac aatgtgttat atcaggatga aggacaagtg
    22681 atgacctccg ccggaagcgc cgcggggatc gatctgtgtt tacatatcgt gcgcaaagat
    22741 tttggtcatg agattgctaa taatgtcgcc cggcgactgg tcattcagcc acatcgccag
    22801 ggggatcaac cacaaaatct gacgcgccca atggcctcac cgcgtgaaag ccagacgtta
    22861 ggtgccttat tcgacttttt gcagcagaat ctggcacaaa cacatactgt gtcttccctc
    22921 gccgaacggg taaatatgag tccgcgcact tttttaagac gttttgaggc gttaactggc
    22981 atgacgcccg cccgctggct gctcaatgag cgcttaatac gagtacagga ttatctgcaa
    23041 aattcggttt ttaacatcga gaaaattgcc gagctgacgg gttttggcaa ttcagcgtta
    23101 ttgcgtcatc actttcacca acgtttttta ttatctcctt cgcaatatcg caaaaacagc
    23161 cgttccccat cacaataatc gcctgattca gcctttgttt agataacctc tgctaatatc
    23221 tttctcattg agataaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc
    23281 aaataatgct gggtaatatg aatgttttta tggccatact gggaataatt ttattttctg
    23341 gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc
    23401 acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa
    23461 aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata gatttgtgtt ttgcttttac
    23521 gctaacaggc attttcctgc actgataacg gatcgttgac acagtagcat cagttttctc
    23581 aatgaatgtt aaacggagct taaactcggt taatcacatt ttgttcgtca ataaacatgc
    23641 agcgatttct tccggtttgc ttaccctcat acattgcccg gtctgctctt ccaatgacca
    23701 catccagagt ctcttcagga aatgcgcgac tcacacctgc tgtcacggta atgttgatat
    23761 gcccttcaga atgtgtgatg gcatggttat cgactaactg gcaaattctg acacctgcac
    23821 gacatgcttc ttcatcatta gccgctttga caataatgat aaattcttcg cccccgtagc
    23881 gataaaccgt ttcgtaatca cgcgtccaac tggctaagta agttgccagg gtgcgtaata
    23941 ctacatcgcc gattaaatgc ccgtaggtat cattaaccaa tttaaatcgg tcaatatcca
    24001 acaacattaa ataaagattc agaggctcag tgttgcgtaa ctgatgatca aaggattcat
    24061 caagaacccg acgccccggc aatcccgtca aaacatccat attgctacgg atcgtcagca
    24121 aataaatttt gtaatcggtt aatgccgcag taaaagaaag caccccctcc tgaaaggcgt
    24181 cgaaatgcgc gtcctgccag tgattttcaa caatagccag cattaattcc cgaccacaat
    24241 tatgcatatg ttgatgggca gaatccatta gccgaacgta aggtaattca tcgttatcga
    24301 gtggcctcag atgatcaatc caccgaccaa actggcacag tccataagaa tggttatccg
    24361 ttatttctgg cttactggca tctctcgcga ccacgctgtg aaacatactc accagccact
    24421 gatagtgggc atcgatagcc ttattgagat ttaacaagat ggcatcaatt tccgttgtct
    24481 tcttgatcat tgccactcct ttttcacagt tccttgtgcg cgctattcta acgagagaaa
    24541 agcaaaatta cgtcaatatt ttcatagaaa tccgaagtta tgattcatct ctgagataac
    24601 attgtgattt aaaacaaaat cagcggataa aaaagtgttt aattctgtaa attacctctt
    24661 cattatcgca aatgaaagga tgacaaatag cataacccag tacccaaatg gcccggtagt
    24721 tcaggccatc aggctaattt atttttattt ctgcaaatga gtgacccgaa cgacagccgg
    24781 cgcgcttttc ttatccagac tgccgctaat gttgatcatc tggtccggct gaacttctcg
    24841 tccatcaaaa acggtcgcag gaataacaac attaatttca ccactcttat cgcgaaaaac
    24901 gtaacggtcc tctcctttgt gagaaatcaa attaccgcgt agtgaaaccg aagcgccatc
    24961 gtgcatggtt tttgcgaaat caacagtcat tttttttgca tcatcggttc cgcgatagcc
    25021 atcttctatt gcatgaggcg gcggtggcgc tgcatcctgt tttaaaccgc cctggtcatc
    25081 tgccaacgca taaggtatga caagaaaact tgctaataca atggcctgaa atttcatact
    25141 aactccttaa ttgcgtttgg tttgacttat taagtctggt tgctattttt ataattgcca
    25201 aataagaata ctgccaattg ttataaggca tttaaaatca gccaactagc tgtcaaatat
    25261 tcagagaatt taactcacta aagtta
//
LOCUS       DAZFTN010000068        24824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFTN010000068 DAZFTN010000000
VERSION     DAZFTN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            51..791
                     /gene="yiaT"
                     /locus_tag="ACHKV2_003072"
     CDS             51..791
                     /gene="yiaT"
                     /locus_tag="ACHKV2_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO6940916.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(921..2891)
                     /locus_tag="ACHKV2_003073"
     CDS             complement(921..2891)
                     /locus_tag="ACHKV2_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO6940917.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2902..4302)
                     /locus_tag="ACHKV2_003074"
     CDS             complement(2902..4302)
                     /locus_tag="ACHKV2_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6940918.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4528..5343
                     /locus_tag="ACHKV2_003075"
     CDS             4528..5343
                     /locus_tag="ACHKV2_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6940919.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5345..5485
                     /locus_tag="ACHKV2_003076"
                     /pseudo
     CDS             5345..5485
                     /locus_tag="ACHKV2_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            5587..6060
                     /gene="ysaA"
                     /locus_tag="ACHKV2_003077"
     CDS             5587..6060
                     /gene="ysaA"
                     /locus_tag="ACHKV2_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6940920.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDYSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6212..7465)
                     /gene="avtA"
                     /locus_tag="ACHKV2_003078"
     CDS             complement(6212..7465)
                     /gene="avtA"
                     /locus_tag="ACHKV2_003078"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO6940921.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7643..9673)
                     /gene="malS"
                     /locus_tag="ACHKV2_003079"
     CDS             complement(7643..9673)
                     /gene="malS"
                     /locus_tag="ACHKV2_003079"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6940922.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVIGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            9993..10817
                     /gene="bax"
                     /locus_tag="ACHKV2_003080"
     CDS             9993..10817
                     /gene="bax"
                     /locus_tag="ACHKV2_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO6940923.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10925..12103)
                     /gene="xylR"
                     /locus_tag="ACHKV2_003081"
     CDS             complement(10925..12103)
                     /gene="xylR"
                     /locus_tag="ACHKV2_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO6940924.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12181..13362)
                     /gene="xylH"
                     /locus_tag="ACHKV2_003082"
     CDS             complement(12181..13362)
                     /gene="xylH"
                     /locus_tag="ACHKV2_003082"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO6940925.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13340..14881)
                     /gene="xylG"
                     /locus_tag="ACHKV2_003083"
     CDS             complement(13340..14881)
                     /gene="xylG"
                     /locus_tag="ACHKV2_003083"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO6940926.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14959..15951)
                     /gene="xylF"
                     /locus_tag="ACHKV2_003084"
     CDS             complement(14959..15951)
                     /gene="xylF"
                     /locus_tag="ACHKV2_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6940927.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16317..17639
                     /gene="xylA"
                     /locus_tag="ACHKV2_003085"
     CDS             16317..17639
                     /gene="xylA"
                     /locus_tag="ACHKV2_003085"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO6940928.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17711..19165
                     /gene="xylB"
                     /locus_tag="ACHKV2_003086"
     CDS             17711..19165
                     /gene="xylB"
                     /locus_tag="ACHKV2_003086"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO6940929.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19334..19675
                     /gene="yiaB"
                     /locus_tag="ACHKV2_003087"
     CDS             19334..19675
                     /gene="yiaB"
                     /locus_tag="ACHKV2_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO6940930.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19721..20158
                     /gene="yiaA"
                     /locus_tag="ACHKV2_003088"
     CDS             19721..20158
                     /gene="yiaA"
                     /locus_tag="ACHKV2_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO6940931.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20200..21195)
                     /gene="wecH"
                     /locus_tag="ACHKV2_003089"
     CDS             complement(20200..21195)
                     /gene="wecH"
                     /locus_tag="ACHKV2_003089"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO6940932.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21370..21669
                     /gene="ysaB"
                     /locus_tag="ACHKV2_003090"
     CDS             21370..21669
                     /gene="ysaB"
                     /locus_tag="ACHKV2_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO6940933.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21764..22675
                     /gene="glyQ"
                     /locus_tag="ACHKV2_003091"
     CDS             21764..22675
                     /gene="glyQ"
                     /locus_tag="ACHKV2_003091"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO6940934.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22685..24754
                     /gene="glyS"
                     /locus_tag="ACHKV2_003092"
     CDS             22685..24754
                     /gene="glyS"
                     /locus_tag="ACHKV2_003092"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO6940935.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6129 a         6115 c         6415 g         6165 t
ORIGIN      
        1 cattactatg ccgactcttt tttatcttat tttaaaatgg aataccggac atgttaatta
       61 accgcaatat tgtggcgtta tttgcgttgc cttttatggc aagcgcaact gcttctgaat
      121 tatccattgg tgctggtgcg gcttataatg aatcgcctta tcgcggttat aatgaaaata
      181 caaaggcaat tccgctgatt agttatgaag gtgattcttt ttatgttcgt cagaccacgt
      241 taggttttat tctgtcgcaa agtgaaaaaa atgaacttag cctgaccgca tcctggatgc
      301 cgctggaatt tgaccctgcc gataatgacg attatgccat gcaacagctt gataagcgtg
      361 atagtacggc tatggcgggg gttgcctggt atcaccacga gcgttgggga accgtgaaag
      421 cctctgcagc tgcggacgtt ctggataaca gcaacggctg ggtgggggag ctatcggtat
      481 tccacaaaat gcagataggt cgtctgtcgc tgacacctgc gctgggcgtt ctctattatg
      541 acgagaattt cagtgactat tactatggca tttcagagag tgagtcccgt cgtagcggtc
      601 tggcaagtta ttccgcgcag gatgcctggg tgccctatgt cagcctgacg gcaaaatacc
      661 cgataggaga gcacgtcgta ttgatggcga gcgcaggata cagcgagctg ccggaagaga
      721 ttaccgacag cccgatgatt gatcgtaatg agagttttac ctttgtcacc ggcgtgagct
      781 ggcgttttta attcaccggt ggatgtcggt gcggctggcc tacgctctcg caccgacttg
      841 tatgcctgat aagacgtgtt aagcgtcgca tcaggcaatg tgctccttac gttggatgcg
      901 gcgcgagtgc cttatccaac ctacggatgg caatgctttt cctcattcac ccagatccgc
      961 atttcgcctt caccccggtt ggcccagcta aaccacggga taaaggtcag agtttgcgtc
     1021 tggcgtttgg ctggcgcgct gtcgtaatgc cacagcggtt gctgctctgg attgctctgt
     1081 tcataccggt aacccggtgc ctggattaag atcttatggc taaacaatcc cttgccttca
     1141 aatgtcgtaa atggcgcatc ggtgggcagc cacagattat gcagtgactc gccgttgtcg
     1201 gccttttcca ggcaatacac cagcgggccg cgctgaatcg ccacttttcc ggcgacgtga
     1261 cgcaccagcg ggttaccgta aacgcggcgt accggcatcg gcaaagtcag attcagcgta
     1321 tcgccctcct gccattcgcg ggtaatgtgc aaataccctt tacgaatatc ctgctcgacc
     1381 tcttccccat tcaatatgat ctgcggctgt gtgcaccagt ccggcagacg taaagccagc
     1441 gtatgacgta ccggctgggg cgattcaacc gcaatcgtca cctgctcctg ccacggatag
     1501 ttcccgctaa cccgcaggcg cagcgtgcca ttttctaccg gcacttccat gctgtttcct
     1561 gcgtagatgt tgatatacaa cgcatcttca cgcggcgtgt agagataatg accaatcgag
     1621 gtcagcacgc gggcgatatt tggcggacaa caagcgcagc caaaccaacg ctggcggatc
     1681 ggtttaacgt gatcgtagat atggttgaat ttcagcgatt ttggatgtac ttccagtgga
     1741 ttcacataga agaaatgttt gccatccaat gccatgccgc cgagcacagt gttgtacagt
     1801 gcgcgctcca tcacatcggc atattgactg tcgccttcca tttccagcat tcgtcgggcg
     1861 aacatcatca ggccgatgga agcacaactt tcggcgtaaa ccgtgtcatt cggcagatcg
     1921 taatcgctgc tgaacgcttc gccgctgctt tgtgagccga tgccgccggt aatatataac
     1981 tgacgctggg ccatattgtt ccacagccgc aggcagtcct gacgcttgct gtcatcgtga
     2041 cttaaacgcg cgagatgcgc gacgccggtc atcagataga caaaacgtac cgcgtgaccg
     2101 atggcggttt gctgttgcgc aaggggcaaa tgtgcctggc tgtaggcttt gtctttcacc
     2161 atccacgccg ggccgtaggt gtgccagtgc gatgtctgcc cgcgcttttc atattcctgg
     2221 tcgtaatagt gcggttgcgc accacgctgt tcgacaaaat agttcgtcag cgccaggtag
     2281 cgcggctctt cagtcacttc atacaggcgc attagtgcca gttcaatttc cgggtgacca
     2341 ggataaccgt gtaacttact ttcatctgga ccaaatacgc tgtcgatatg atcggccagg
     2401 cggcaaacga cccccagcaa gcgccgtttt cccgtggcct ggaagaaggc gactccggct
     2461 tcaatcagat gacctgcgca gtaaagttca tgacactccg ccagattgct ccagcgttct
     2521 tcgggtgctt ttaccgtaaa gtaagtattg agatagccgt cttcgcattg ggcggaggcg
     2581 atcagttcga ttacctcgtc ggcggttttt tccagttcgg cgtccggctt ctggcacagc
     2641 gaccaggcta ccgcttccag ccatttggcg acgtcgctgt cctgaaacac catcccgtaa
     2701 aattcaccct cctgaagtcc ggcggcaatg cgaaagtttt caatcgcatg gctgggttcc
     2761 gcttctggga tacgatcgtt caaggcatcc cactgataag gaatcaccac gtcgcggacc
     2821 agttgttggt actgaccgag gaacggatcg ctgaccgtca gtttatgcag atcgacttcc
     2881 gaaatgttca tcttgtgctc cttaaggctg tacatgacgc tgacgcaggt cggtagctat
     2941 gcgtgacatc ataggattat tgagtttgca aaagcggatc gcccctgcca gcagcaggtg
     3001 gaatagcgca ggcagtaatg tttccatcgc cgcgatcccg ccaagcgaac tggcggtctg
     3061 gttttccacg ccaggctggt aggcgacaaa aataaacagc aggctgatga tcccggcgct
     3121 ggaggcccag gccagcttga tgaaaaacag attgaaggcg aagttcatgc cggaagaacg
     3181 tacgtgggtt ttccactcgc cgtagtcatc ggcaaaggcc atcaatgaga agtgcagtgg
     3241 caatgtgaag ccaagaatca cgccattacc gaggatcacc gccagccaca gcgtttgata
     3301 agccgggccg gagggcagga accacatcaa caccgccagt gcagcgagga gcaggttggt
     3361 gtagtaataa attttgacgg tatcgaaacg ccgcgttaac gggctgacaa tcaccgagcc
     3421 gataatggag gcgaaggtga ccatggtgaa gaacagcgac gtgtagcccg tgctgccttg
     3481 taagacgtag gtaatgaagt acatataccc accgccgcga atgttaaaga cgttaatcag
     3541 caggaaagac atgaccagca tcagcagcag ttggtcgttg ttccgcagcc cggcaagatg
     3601 ttcgcgcagg gtaaatttcc ccattgtgga gagcggcacc cgttcacgaa cccagaagaa
     3661 acagcacagg aacatcacca cggcaatggc gcacaatacc ccgacgccca gttgatagcc
     3721 gcgtgcagcg ttgccctgac cgaagagagc taccatccac ggtaagccta ccgaaaccag
     3781 aaatcccgct acgccgcaga gaacgaatcg ccaggactgg caggagatca cttcattgtg
     3841 gcgggtggtc atggtgttga tccgcgcgca gtacggtacg ttgatggcgg tataaccgac
     3901 tgaaaggagc aggtaagtac cgaacgccca ggcgattttt actcccatgc tggcatctgg
     3961 cacggtgaac gtcaggatgc cgataatgcc gatgggaatg gcaatccata gctgccacgg
     4021 acgaaagcgc ccccatcggc tttgcgtccg gtcggcaatg acgcccatca ccgggtcgga
     4081 gatggcatca aacacgcgca gtgcaataaa cagcgtgcca accagcgccg gagttaatcc
     4141 aaaaacgtcg gtgtagaaaa aggtgagaaa attcatgatc aggcaagtta ttacggtgcc
     4201 gcctgcgtca cccaggccgt agcctatttt ttcccgtaca gataacgcat cgtcaatttt
     4261 acctttagcg atatcagcgg tagtaatcgg tgtagaggtc atagatattc ctcgtgaata
     4321 gggagcaatt tttagtttta ttgaaatgac ctgattaatt ctgccgtgaa cgggaatatc
     4381 aacaaggcga gaaaagagta aaaaaaggtg acgattccga cctgaattta aaagtgtgaa
     4441 atggatcagg tgaaattaat gtttttgatt aataatcaaa aggtaatgtg tttaatcatt
     4501 aatgaataag cgggaaatta catatcaatg ctcgaattat ccatgacgtt accgattaaa
     4561 gtgcaaaatg gtggattatt tatttcccgt ggcgtaggga ggcattctgc aaggcggctg
     4621 gagtcatggg aaattatctt tgtcgaaaaa ggtcgattaa cgattcagga agaagaacgg
     4681 atatttctgg tcgatgcggg agagagttta ttactctggc cgaaccgaca acatgttggg
     4741 gtggaggatt ttcccgccga tctcaaattt tactggctgc attttgaggt taaagcgccg
     4801 gacaacgacc ctcgctggct tactcatctt agcgtgccgc aacaaacgaa ggttgtcgat
     4861 ccgcaggcgc tggtggccct gtttcgccag ttcttaagcg aacaggaaaa gcatcaacgc
     4921 actccggcgc tggagtttat tgtgctgctc attttgcagc agctcacccg tgacgcgcgt
     4981 cttgatgaac aggccgatgg cgcaggcacc acccttgcct ggaaagcgca gaaactgatc
     5041 cgcacgcaat atcatcgccc gctttctacg gcgcttctgg cccgcgagct gtactgcaat
     5101 gccgattatc ttgggcgcgt ctaccggcgg gtgtttcatc tgacgttaac agaagcaatc
     5161 catcgccagc gggtgcgcga agccgaaaag ctgttaatta gcgaagcgcg atcgctcacg
     5221 gaagtggcgg cgttgtgcgg gtttaatgac gttggctatt tccggcagat ttttcgcaaa
     5281 cacaccggtt taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg
     5341 tagtgccggg gcgatttcgc tttcgacatc acgtctgaaa caggtggggg agaaaaatct
     5401 cctgaaacca ctgcgtgaaa ccgcgcaggc tatttctaat gaactgggat ttactgtcag
     5461 ggacgaccag ggcgcgatca cataacgctt tggacaagtg ccaaaacttt aacatttcct
     5521 tcgttggatc aaagcagtag ggacgcgctc tctggcactc tgctgtttta gtgcaaagga
     5581 gtgatcatga accggtttat tattgcggat gcgacgaaat gtatcggttg ccgtacctgt
     5641 gaagtggctt gcgcagtgtc gcatcaggag aatcaggatt gcgctgcgtt gtcaccagac
     5701 gagtttattt cccgtattcg tgtcattaaa gactacagct ggaccacggc agtagcctgt
     5761 catcagtgtg aagatgcacc gtgcgcgaat gtctgccctg ttgacgcgat aagccgcgaa
     5821 catgggcata ttttcgttga acaaacacgt tgcattggct gtaaaagctg tatgctggct
     5881 tgcccgtttg gtgcgatgga ggtcgtttct tcgcgcaaaa aggcgagggc gattaagtgc
     5941 gatctgtgct ggcatcggga gacgggaccg gcctgtgttg aagcctgccc gacaaaggcg
     6001 ttgcagtgca tggatgtcga gaaagtgcag cggcatcggc tacggcagca gcctgtttga
     6061 aacgtttttc cggcgtttct caacgccgga atagtgcgct ttgtttatgc cagatgcggc
     6121 gtaaacgcct tatccggccc acaaattctt gcaaattaaa tatattgcag aaatcatgta
     6181 ggcctgataa gcgtagcgca tcaggcaatt tttagtgact ttcagcccag gctctttcta
     6241 tctcttccgc cagaatcttc acccccgcct caattttctc cggctctggt acgtagttca
     6301 tgcgcataca ttgatgcgta tgcggccacg gtttgtccag ccctgggaag aagttgtgcc
     6361 ccggcaccat cagcacgccg cgtgctttca ggcgctgata gagctgctcg gtcgtaatgg
     6421 gcaaatcctt aaaccaaagc cagaggaaaa tggctccttc tggtttatga attaggcagc
     6481 gatcttccgg taaatagcgg cgaatgatgg cgatagtttc ctgaacacgc tggtagtaaa
     6541 acggtttgat gactgtttca gacaggcgca gcagatcgtt acgcttaatc atttcacaca
     6601 tcatcgccgg accaataccg ccaggtgcca ggctgataat gccgttcata ttggtgatgg
     6661 cggtgatgat tttttcattg gcgatgataa tgccgcagcg ggagccaggc agccccagct
     6721 tggaaagact catgcacagc acgatattcg gattccatag cgggcgcgct tcactgaaga
     6781 tgatacccgg gaacgggacg ccataagcgt tatcaatcac cagcggaatg ccgtgttgat
     6841 tcgccagcgc gtcaagcttc agcaactctt cgtcagtaat cacattgcct gttggattcg
     6901 tcggccggga gacgcaaatc atcccggttt cttcgccaat atgcagatgc tcaaaatcga
     6961 cgtggtattt aaactggcct tccggcagca gttcaatatt cggacgcgca gagacaaaca
     7021 gatcttcttc cagtccggcg tcagcgtagc caatgtattc cggtgccagc gggaacagca
     7081 cttttttgac ccgaccatcg gcacggcgtc cggcaaacag attaaataag tagaaaaacg
     7141 cgctctggct gccgtttgtt agtgcaatat tctgtgcttc gatatcccaa cccaacttct
     7201 cgcgcagcat tccggcaagc agtgtgagta gctccgtttt cccctgtgga ccgtcgtaat
     7261 tgcacagtgc atcagtcgct ttgccacttt ccagcatgtc ggtcagtagc gtctggaagt
     7321 agtcctgcat ttccgggatc tgcgccggat taccgccgcc gagcataatc gcgccaggcg
     7381 tgcgtaaacc gtcgttcaga tcttccatca gcagcgtaat gccggagtgg cgggtaaatt
     7441 tgtcaccaaa aagggagaat gtcatagcgg gatatctgtc gaactctgta gcaaggaagg
     7501 taacaataac gctacatttg ctggggtgca aatcggtctg ttgaagagtg agcggtgctt
     7561 tatcctgcaa cgctgattag ggctgacatt ttatcctggt tggcggcttc ccgaggtact
     7621 gacgaacggg aagccggaaa agtcactgtt gcccagccca gatgaccagc actttatcgt
     7681 cgccatgctc acgaacaaag ccgtagccct gcttcagcga aagtgtcgtt tgtttgcccg
     7741 cgccaattgc gggatggcgg gcgcggaact ggctgatttt ctgccagtgc gcgacgttgg
     7801 cggcagattt accgataaca tcctgccagt tcatatccga acgtgtacct tgcagcggat
     7861 cagaacctgt aggaccgaac ggacgcgagg attcatcgcc ataaaagatt tgtaccgcgc
     7921 ctggcgctaa tagtaataac tctgctgctt tgtcgccccc ttcacggaac agacgggtat
     7981 catgcgacga gaggtagctc aacacgttga aaccctgcaa tttctccgcc atttgctgcc
     8041 aggtcgtatc catctgcgca atacaatcga cagctttcgc cgcctgctcc tgataatcga
     8101 aattgatcat cgcatcgaag ccgtggcgat agtagtcact ttgcatcacg ccgtggcccc
     8161 aggcttcacc ggtcatccag aaaggtttgt catctaatgc tttgtcgggg ttagcttttt
     8221 tccattcgcg aagcgcggcg ctggcttcgg ttttcagttg ctgccaagcg ggcaactcaa
     8281 catgtttggc ggtatcgacc cgaaaaccat caatcccata gtcgcggacc cactgactta
     8341 accagtgggt taagtaatcg cgaggggtaa agccgtcgat ggctttagcg tgggtatccg
     8401 ttttgttttt atagaacacc ggcagaccag aagcggtagt tgattcggtt ttgatatccg
     8461 gcaaaaaggc tagcgacatg gtgagatcgt cgaatccagg attgtcgtaa tcgccgatat
     8521 cggtacggat ccagtttttt ccccaccatt tatcccagcc tgttttgtcg ctgaaattaa
     8581 tgtaatcgtt aaagctatgc caggtttgcc cggcggcagg tttccagtcg ctccagcgtt
     8641 cacccagcgt ttttttcact tcgtcaccag aaagatataa cgcgccaaac tgatactcct
     8701 gcatatccgc cagcgtggca tagccggtgt ggttcatcac gacatcaaag agaatacgaa
     8761 taccgcgctg atgtgcgcta tcaaccagcg tccgtagatc ggcttcgttg cccatattgg
     8821 catcaagatt cgtccagtcc tgtgtgtaat aaccgtggta ggcataatgc gggaaatcgc
     8881 cttttgtacc gccgccgacc cagccgtgaa tttgctcaaa tggggcgctt atccataaag
     8941 cattaacgcc caactgctgg aggtaatcca gtttgttggt caggccgcgt aaatcgccgc
     9001 cgtgaaaagt gccaatttcc gccataccgt ctttatgacg tccgtaactc tggtcattac
     9061 tgggatcgcc gttttcgaaa cgatctgtca gcacaaagta aaccgtggcg ttatgccagt
     9121 cgaaaggggc agaggcgtca gtttctgccc gttccagcag gagtaaaccg ttgctggtag
     9181 cagcgggttg taacgttatt tgaccgttct tcactatcgc aatttgctgg ctgtaataat
     9241 cccgtacggc ggctccttcc gggaaagtgg cgctgacatc cagcgtgagc ggtaatccat
     9301 cccatttcgg gcattcacgg accaggtttg ctaccggttc ggcggcgttc tggatggaaa
     9361 tcatcaatgt tggcgtaccg gagcgggtgt ctatttgcag cgtatatttg ccgtccctga
     9421 acaatcgcca ttgaggcggc gtgttgctac aaggttgcag ggaaagcatc tgattgagtt
     9481 ttatcgcatc tgcaggctgc cagcactgct ggtcaaaatt tagcgtgagt ggacgcgtac
     9541 ctttgggcaa ctgcgcgtgg ctgacaaatg ttcccgttcc ctgttcgcta aaggcaggga
     9601 accccggaga agtccagctg gcggcaacgg cgaagccagg aaggagtgtc agaaaacagg
     9661 cggcgagttt catggtggat gagtccttat tgctgcgatt tcagacagtt tgccagcgat
     9721 aagccagaca aaactcatcc ctgagcggta agtccacagg atgagaagca agggtgagcg
     9781 atcgcgcgca aaaaacggct gaattttgcg ataaacccca cattttctgc gatttagcgc
     9841 caatctgaat cgttaactcg tgacatagtt tcagatttgg actattcctt ggggtgctct
     9901 tgacagctat ttttgatatg atttgagatt caactctcaa atttgtgaaa aatataaggt
     9961 gttggaatga ttaaatccga ccaggagacc tgatgatttt gactcccata cgacgatatg
    10021 gggcgatgat tcttatgtta ctcactctgg tgttttcgag tgaggtgtta gcgaagacgc
    10081 acacaacaac agcgagtcaa aagtcccact taactaaagc tagtaataaa caggtaagca
    10141 gtaaacaaga gtattctcgc aatagtgcaa agagtagttc acttcctgat ttgcgaaaat
    10201 acccttccgg aacatcaagg aaaaaggcgt ttctccggac cgtaatgcct tacattacca
    10261 gccaaaatgc agccattact gcagaacgta actggctgat ttcaaaacag tatcagggcc
    10321 aatggtcacc tgctgagcgt gcgcgtctga aagacatcgc caaacgctac aaggtgaagt
    10381 ggtctggtaa tacgcgaaaa atcccgtgga ataccttgct tgaacgcgta gacattatcc
    10441 ctaccagtat ggtggcgacg atggctgcag cagaaagcgg ttggggaacg tcgaagctgg
    10501 cgcgcaacaa caacaatctt ttcggcatga aatgcatgaa aggacgttgt accaatgcgc
    10561 caggtaaagt gaaagggtac tcacagttta gttctgtcaa agaatcggtg agcgcctatg
    10621 tcactaacct caatacgcat ccggcttact cttcgttccg taaatcgcgt gcgcagctgc
    10681 gtaaagctga tcaggaagtg actgccacag cgatgattca caagctgaag ggctactcga
    10741 ccaaagggaa gagttataac aactacctgt tcgcaatgta ccaggataac caacggttaa
    10801 tcgcggcgca tatgtaattg tatttcctcg agccttatcc gacttgtcgg tcggataagg
    10861 cttttgatcg catcaggtgg ttgtgctgag ttacctgatg cgacgccgac aattctcatc
    10921 atcgctacaa catgacctcg ctatttacat cgcgatactc ttttggcgtc gtgtcatatg
    10981 cttttttaaa aacagagtag aaatattgca gcgatggata accgcacatt tgcgatatct
    11041 cattgatcga caaggtggtt gaaatcagca gactgcgcgc tttctccagc ttctcagcat
    11101 gaatcatggc atggatggtt tcacccacct cttctttaaa acgcttctca agattggagc
    11161 gcgagatccc gaccgcatcg agtacctgat ccactttaat ccctttacag gcgtgattac
    11221 gaatgtaatg catggcctga ataacggcgg gatcggtcag cgaacggtaa tctgttgagc
    11281 gccgttcaat gacgcgaact ggagggacca aaatccgctg tagcggcatt tcttctttat
    11341 ctaataatcg atgcaacagt tttgccgcct gatagcccat ttgccgcgcg ccctgagcga
    11401 ccgaagaaag ggcgacacgc gacagatagc gggtcagttc ttcgttatcg atgccaatca
    11461 cgcataattt ttccggtacg ggaatgtgta gatgttcaca tacttgcaga atatgccgcg
    11521 cccgggcgtc agtaacggca ataatcccgg tttgcggtgg cagtgtttgt agccagtctg
    11581 ccagccgatt ttgcgcgtgt tgccagttct ctggtgcggt ttctaacccc tgataaacca
    11641 ctccgcgata cttttcttcg gcgacaagct gacgaaatgc atattcgcgt tcagtggccc
    11701 aacgtttgcc gcttgattcc ggaagaccat aaaaagcaaa gcggttaacg cctttctctt
    11761 ttaaatgcaa aaatgcgctt tcaaccagcg catagttatc ggtggcaatg taatgaacgg
    11821 gtgggtaact ttctgcaagg tgatacgagc cgccaacccc aacaatgggg acgtcgacat
    11881 cagccagcgc ttgctcgatc tgtttgtcgt cgaagtcggc aatgacgcca tctcctaacc
    11941 agtccttgat tttatcaatg cgggcgcgga aatcttcttc aatgaaaata tcccattccg
    12001 attgtgacgc ctgtaaatat tcccctacgc cttctactac ctgccggtca taggctttat
    12061 tggcattgaa cagtaatgtg atgcggtgac gtttagtaaa catggttctt ttcctgctga
    12121 atcatgcaaa aactcaaaac cggtaatacg ttaccggctt tgagaaaatt tttatcaaaa
    12181 tcaagaacgg cgtttggttg cggagtccat ccatactgcc agcaacagaa tcgcaccttt
    12241 aacgatatac tgccagaagg tcggtacatc catcatactc ataccgttat ccagtgaagc
    12301 catgatgaat gcccccatta ccgctccagc cacgctcccg acgccgcctg ccagactggt
    12361 gccgccaatc acgcatgctg caattgcgtc cagctcggcg atattccccg cagaaggtga
    12421 accagcgcca agtcgagaac tgaggattaa tccggcgatg gctaccatta atccgttaat
    12481 agcgaacacg gcaagtttgg tgcgttcaac gttaattccg gagagacgtg ctgcttccag
    12541 attgccgccg atggcataaa tgcgtcgtcc aaatgctgtc cgcgttgcca taaacattcc
    12601 gccgagtaac agcaacgtca gcagcagaac aggagtagga acgccacggt aatcattcaa
    12661 cagccagatt gcgcctaata cgatgatagc ggttaaagcc tggcgaccga ctactgcggt
    12721 agaggctgga gactgcaaac ccaaagcctg acggcgcatt cttccgcgcc attgccagcc
    12781 aacaaaagcc attaagccaa gcgcgccaat gatgaagccg gtgctggcgg ggagatagct
    12841 ttgcccaatt tgtgacatcg cggcgctggt gggggataca gtcgtgccgt tggtgatgcc
    12901 aatgagtatg ccgcgaaatg ccaacatgcc cgcgagagtg acaataaatg aagggacttt
    12961 gcggtacgcg acccaccatc cgttccaggc accgagaagc agtcccagaa ccaacgtcac
    13021 aatgatggta agtggcaaag gccagcctag ccagacgtca caaatcgccg cgacgccacc
    13081 taatagcccc atcattgagc cgacggaaag gtcgatttca gcagaaatta tgacgaacac
    13141 cattcctacc gcgaggatgc cggtaatcgc ggtctggcgt aacaggttgg agacgttacg
    13201 ggcgcttaag taggcaccat cggtggtcca ggtaaagaac agcatgattg cgatgatagc
    13261 tgcaatcatc acgaagacct gcaaattcag tgatttcagc ccggagaagc caccggatgt
    13321 cggtacggcc aatttcactt cagacggatt gcttttcgac atgatgttcg ctcctcaatg
    13381 cggcttccat cacctgctcc tgagtcaggt tatgatttat caggttggct tttagtttcc
    13441 cttcatgcat tactagtaca cgatcgctaa ggccgagcac ttcaggtaat tcagaagaga
    13501 tgacaataac ggcaataccc tgctggacga gttggttaat taatttgtag atctcgtatt
    13561 tcgcgccaat atcgataccc ctggtgggtt catcaagaat gagaatgcgc gggttaagta
    13621 acagacagcg agcgaggatc gctttttgct gattgccgcc gctcaaacgt ccaatagcaa
    13681 ggtcggggga cgacgtttta actttgagtt gctggattga ttccagaata catttttgct
    13741 ctgccgcgtc atcaagctgg ctaatgccac cggtaaattt attgagtgcg gcgagggtaa
    13801 tatttttacc aaccgccatt accggaacga tgccgtcgcg ctttctgtct tcggggacca
    13861 tcgcaatccc ctgggcgatg gcttgctgac agttacgaat atctacctgt ttgccatcaa
    13921 tataaatttt tccttcccat tgtccgggcc acacaccaaa caggcactga atggtctcgg
    13981 tacgtccggc accaacgagt ccggcaatac ccagtatttc gccacgtttc agggaaaacg
    14041 agacatcatt aactcgttta atatgacgat taaccggatg ccatgccgtc agatgttcaa
    14101 tacgtaatat ttcatctccg gtggtatgtg gttcattagg gtaaagcgcg gttaactctc
    14161 gcccgaccat catggtgata atatcgtctt cactcattcc ggcagcatca cgcgtaccaa
    14221 tgtgctgtcc gtcgcgaata acgcaaatcg tatcggaaat cgctttgact tcgttgagtt
    14281 tgtgcgaaat ataaatacag gcgataccgt gctgttgtag atcgcgaata atatccagta
    14341 aaaccgacgt ttcctgctca gttaatgagg ctgtcggttc atcgagaatt aacaagcgca
    14401 cctgtttatt aagtgccttg gcaatttcaa ccagttgttg ttgcccaagc cctaaatcgc
    14461 caacgcgggt atcaggtgaa atggataaac tgacctgtgc gagcagcttc tgacagcgta
    14521 gcgtcatcag gtcataatcc ataatgccat tgtgggttat ttcgttaccc aggaagatat
    14581 tttccagcac ggtcaattct ttcaccaggg ccaattcctg gtgaatgatg gcgatacctt
    14641 tgcgttcggt atcgcggatg tgactcgcct gaatctcttc tcccgcaaaa ataatttcgc
    14701 cttcgtagga gccatgggga taaataccac acagcacttt catcagcgtt gatttaccag
    14761 acccattttc cccacagagt gagacgattt cgccagcatt caaccgcaag ctgacgttat
    14821 caatcgcctt cacactgccg aaggttttgg taatgttctt catttcaagt agataaggca
    14881 tagcgactcc acctaagcca attcattcac gcggcatgga gagaaatcac gcccccgctc
    14941 cgcgctgggg cgtaacgctt acagctcgct ctctttgtgg aatccgtctt taattaccgt
    15001 atctttgatg ttgtttttat tcacatcgat cggtgtcagg aggcgggagg ggacatcttt
    15061 caggccatta tttagtgaag tatctgcttt tggctcctga ccattgccca attcaacggc
    15121 aatttctgcg gcagtatttg ccagcaatgt aataggttta tataccgtca tagtttgcgt
    15181 accggcagca atacgtttaa tacctgcgag atccgcatcc tggccagaaa ttgctacttt
    15241 ccctgataaa ccttgcgcgc ttaatgcctg aattgcccca cctgcggtgg catcgtttga
    15301 ggcaactaca gcatcaattt tgttattatt ggcggttagc gcgttttcca taattttcaa
    15361 tgcgttttcc ggtaaccagc catcaaccca ttggtcacca acgactttaa tttttccgga
    15421 atcaacgtaa ggttgtaaca ctttcatttg tccggcgcgg aacagcttag cgttgttatc
    15481 taccggcgag ccgcccatca ggaagtaatt accttgcgga acaatatcga ccagggcttt
    15541 tgcctgcagt tcaccgactt tttcgttatc gaaagaaata taaaaatcga tatccgcatc
    15601 gttaatcata cggtcgtaag ctaatacttt aatgccttct tgtttggctt cttttacaac
    15661 gttacttaat acctgaccgt tatacggaat aatgacaaga acatcgacac cccggtttat
    15721 catgttttca atctgcgaca tttgtgtttc ttcattgcca tttgcagact gtacaaatac
    15781 tttcgcgccg agagattctg cctttttcac aaagatatct cgatcttttt gccagcgttc
    15841 aagacggaga tcatcaatcg ccatacctat tttgacttct ttggcgtgtg cagcaacgtt
    15901 ggtaagcagg agtgaggtgc aaagggtgag tagaatgttc tttattttca tggtgtaggg
    15961 ccttctgtag ttagaggaca gctttaataa gtaacaatca ccgcgataaa cgtaaccaat
    16021 ttttagcaac taaacagggg aaaacaatta cagattttta tctttcgatt acgatttttg
    16081 gtttatttct tgatttatga ccgagatctt acttttgttg cgcaattgta cttattgcat
    16141 ttttctcttc gaggaattac ccagtttcat cattccattt tattttgcga gcgagcgcac
    16201 acttgtgaat tatctcaata gcagtgtgaa ataacataat tgagcaactg aaagggagtg
    16261 cccaatatta cgacatcatc catcacccgc ggcattacct gattatggag ttcaatatgc
    16321 aagcctattt tgaccagctc gatcgcgttc gttatgaagg ctcaaaatcc tcaaacccgt
    16381 tagcattccg tcactacaat cccgacgaac tggtgttggg caagcgtatg gaagagcact
    16441 tgcgatttgc cgcctgctac tggcacacct tctgctggaa cggggcggat atgtttggtg
    16501 tgggggcgtt taatcgtccg tggcagcagc ctggtgaggc actggcgttg gcgaagcgta
    16561 aagcagatgt cgcatttgag tttttccaca agttacatgt gccattttat tgcttccacg
    16621 atgtggatgt ttctcctgag ggcgcgtcgt taaaagagta catcaataat tttgcgcaaa
    16681 tggttgatgt tctggcaggc aagcaagaag agagcggcgt gaagctgctg tggggaaccg
    16741 ctaactgctt tacaaaccct cgctatggcg cgggtgcggc gacgaaccca gatccagaag
    16801 tcttcagctg ggcggcaacg caagttgtta cagcgatgga agcaacccat aaattgggtg
    16861 gtgaaaacta tgtcctgtgg ggcggtcgtg aaggttacga aacgctgtta aataccgatt
    16921 tgcgtcagga gcgtgaacaa ctgggccgct ttatgcaaat ggtggttgag cataaacata
    16981 aaatcggctt ccagggcacg ttgcttatcg aaccgaaacc gcaagaaccg actaaacatc
    17041 aatatgatta cgatgctgcg acagtctatg gcttcctgaa acagtttggt ctggaaaaag
    17101 agattaaact gaacattgaa gctaaccacg cgacgctggc aggtcactct ttccatcatg
    17161 aaatagccac cgccattgcg cttggcctgt tcggttctgt cgacgccaac cgtggcgatg
    17221 cgcaactggg ctgggacacc gaccagttcc cgaacagtgt ggaagagaat gcgctggtga
    17281 tgtatgaaat tctcaaagca ggcggtttca ccaccggtgg tctgaacttc gatgccaaag
    17341 tacgtcgtca aagtactgat aaatatgatc tgttttacgg tcatatcggc gcgatggata
    17401 cgatggcact ggcgctgaaa attgcagcgt gcatgattga agatggcgag ctggataaac
    17461 gcatcgcgca gcgttattcc ggctggaata gcgaattggg ccagcaaatc ctgaaaggcc
    17521 aaatgtcact ggcagattta gccaaatatg ctcaggaaca taatttgtct ccggtgcatc
    17581 agagtggtcg ccaggagcaa ctggaaaatc tggtaaatca ttatctgttc gacaaataac
    17641 ggctaactgt gcagtccgtt ggcccggtta tcggtagcga taccgggcat ttttttaagg
    17701 aacgatcgat atgtatatcg ggatagatct tggcacctcg ggcgtaaaag ttattttgct
    17761 caacgagcag ggtgaggtgg ttgcttcgca aacggaaaag ctgaccgttt cgcgcccgca
    17821 tccactctgg tcggaacaag acccggaaca gtggtggcag gcaactgatc gcgcaatgaa
    17881 agctctgggc gatcagcatt ctctgcagga cgttaaagca ttgggtattg ccggccagat
    17941 gcacggagca accttactgg atgctcaaca acgggtattg cgccctgcca ttttgtggaa
    18001 cgacgggcgc tgtgcgcaag agtgcgcttt gctggaagcg cgagttccgc tatcgcgggt
    18061 gattaccggc aacctgatga tgcccggatt tactgcgcct aaattgctat gggttcagcg
    18121 gcatgagccg gagatattcc gtcaaatcga caaagtatta ttaccgaaag attacttgcg
    18181 tctgcgtatg acgggggagt ttgccagcga tatgtctgac gcagctggca ccatgtggct
    18241 ggatgtcgca aagcgtgact ggagtgacgt catgctgcag gcttgcgact tatctcgtga
    18301 ccagatgccc gcattatacg aaggcagcga aattactggt gctttgttac ctgaagttgc
    18361 gaaagcgtgg ggtatggcga cggtgccagt tgtcgcaggc ggtggcgaca atgcagctgg
    18421 tgcagttggt gtgggaatgg ttgatgctaa tcaggcaatg ttatcgctgg ggacgtcggg
    18481 ggtctatttt gctgtcagcg aagggttctt aagcaagcca gaaagcgccg tacacagctt
    18541 ttgccatgcg ctaccgcagc gttggcattt aatgtctgta atgctgagtg cagcgtcgtg
    18601 tctggattgg gccgcgaaat taaccggcct gagcaatgtc ccagctttaa tcgctgcagc
    18661 tcaacaggct gatgaaagtg ccgagccagt ttggtttctg ccttatcttt ccggcgagcg
    18721 tacaccacac aataatcccc aggcgaaagg ggttttcttt ggtttgactc atcaacatgg
    18781 ccccaatgaa ctggcgcgag cagtgctgga aggcgtgggt tatgcgctgg cagatggcat
    18841 ggatgtcgtg catgcctgcg gcattaaacc gcaaagtgtt acgttgattg gtggcggggc
    18901 gcgtagtgag tactggcgtc agatgctggc ggatatcagc ggtcagcagc tcgattaccg
    18961 tacggggggg gatgtggggc cagcactggg cgcagcaagg ctggcgcaga ttgcggcgaa
    19021 tccagagaaa tcgctcattg agttgttgcc gcaactaccg ttagaacagt cgcatctacc
    19081 agatgcgcag cgttatgccg cttatcagcc acgacgtgaa acattccgtc gcctctatca
    19141 gcaacttctg ccattaatgg cgtaaacgtt atcccctgcc tgaccgggtg ggggataatt
    19201 cacatctata tatctcagca attaattaat atttagtatg aatttattct gaaaatcatt
    19261 tgttaatggc atttttcagt tttgtctttc gttggttact cgtaatgtat cgctggtaga
    19321 tatggagatc gttatgaaaa cctcaaaaac tgtggcaaaa ctattatttg ttgtcggggc
    19381 gctggtttat ctggttgggc tatggatctc atgcccattg ttgagtggaa aaggctattt
    19441 tcttggcgtg ttaatgacag caacttttgg caactatgca tatcttcgcg cagaaaaact
    19501 cgggcaactg gataattttt ttacccatat ttgccagtta gttgcgttaa tcactatcgg
    19561 tctcttgttt atcggtgttt taaatgcacc tatcaatgct tatgaaatgg tgatctaccc
    19621 catcgccttt tttgtctgct tgtttggtca aatgcgtttg tttcgctcgg tatgagcaac
    19681 ataaagctct tacacattca ggaatgaaag gaatactgtg atggacaaca aaatatcaac
    19741 ctattcaccg gcctttagta ttgtgtcatg gatagctctc gttggtggta tcgttaccta
    19801 tctgttaggg ctatggaatg cagagatgca gttaaatgaa aaaggatatt attttgccgt
    19861 actggtatta ggactgtttt ctgcggcgtc ttatcaaaag accgttcggg acaagtatga
    19921 aggcataccg accacttcca tttattatat gacctgcctg actgtcttta ttatctctgt
    19981 tgcattactg atggtaggtc tgtggaatgc gacattatta ctcagcgaaa aaggttttta
    20041 tggactggct ttcttcttaa gcttgtttgg tgcagtagcg gtgcagaaaa atattcgtga
    20101 tgccggaata aacccaccaa aagaaacaca ggttacccag gaagaataca gcgaataact
    20161 cacgtaagcc cggtcagtcc aatgtgaccg ggcttttact taactcacta atctgtttct
    20221 gtcgattcgt tgtaccagca tagaaagtaa caaactcgct gccaacgtcg cgcaaaaaat
    20281 ccaaataata tccagtattg gccaattttt aagctcaatt ccccgggtgc gcagcgcatg
    20341 gataatcaag gcgtggaatc cgtatatacc caacgaatgg cgggagatta agccaagtcc
    20401 gcgaatggta cgcgtatcca gcgtgttttt aaccagagtc aatagcgcga ttgcgcagat
    20461 aaaaaccatc ggcccacagt aaggatacca ggtatcggca aaatttccgc gccactgcaa
    20521 ttcatataat gtcccgcgag agataataaa aactcccgtc gcaaacagtg cggcgctcac
    20581 ccacgacagt gctttatgct gtgtgtccat catccctaaa gcgcggccca acatgccata
    20641 cagaatgtag taaaaagtat cgccattgat atataagtta attggcagcc attcaaaacc
    20701 gtcgattttc tgcggcacgg tgtttgggtt agcgatgata ccaatcacca ccattagcac
    20761 cagcaacatt tttccgccga cgttcttcac ctgaatcagc ggtgaaacca gataaatcac
    20821 cgcaatcgcg aagaaaaacc ataagtggta aaacactggc ttttgcagca ggtttttcag
    20881 cgctaactcc acattaatgg aggtaaacag tgcaatgtag agcagcgcga ttgcgctata
    20941 aaaaaacaga cataagccga tacgcaagaa atggcgcggc tgggcgctgc gttcgccaaa
    21001 aaagagatag ccggaaatca tgaaaaatag cggcacgctg acacgagagg cagagttcag
    21061 aacattggcg atatcccagg tgacggggct aacactatga gcattggtca cataccaggt
    21121 agtggtgtga atcatcacca ccattaaaca cgctatccct cgcaggttat caatccagta
    21181 aattttgggc tgcatctgtg tctctgtatc tggttaaaaa aagtctgacc gataaatcat
    21241 tcagttggcg cactggaata atctgagttt taacactaca gcttatagag gcttaaggaa
    21301 attcgtaaga tatcagccac tataccgata taaataataa gactcacctg caaaccagac
    21361 ggtaatttaa tgatgatgaa ctctttcttt ccggcaatgg cgcttatggt gctagtgggt
    21421 tgttctacac cgtcacccgt gcagaaagca caacgggtaa aggttgatcc tctgcgttcg
    21481 ttgaatatgg aagcgttatg caaggatcag gcggcaaaac gttataacac cggcgagcaa
    21541 aaaatcgacg tcaccgcctt cgaacagttc cagggaagct atgaaatgcg cggttatacc
    21601 ttccgtaaag agcagtttgt ctgttctttt gacgcagatg gccatttttt gcatctttcc
    21661 atgcgttaag ccctgctttt tcccgtttca tactgtatat cttccatcca gcgggtatac
    21721 tgatcccttc ctttaaatcc acacgtatcc agcacgaaat aatatgcaaa agtttgatac
    21781 caggaccttc cagggcttga tcctgacctt acaggattac tgggctcgcc agggctgcac
    21841 cattgttcaa ccattggaca tggaagtcgg cgcgggaacc tctcacccaa tgacctgtct
    21901 gcgcgcgctg gggccagaac cgatggcggc tgcttatgtt cagccttctc gtcgcccgac
    21961 cgatggtcgc tacggcgaaa accccaaccg tttacagcac tactatcagt tccaggtggt
    22021 cattaagcca tcgccggaca atattcagga gctgtacctt ggttctctga aagagctggg
    22081 catggacccg actatccacg atatccgttt cgtggaagat aactgggaaa acccgacgct
    22141 gggtgcctgg ggactgggct gggaagtgtg gctaaacggc atggaagtga cgcagttcac
    22201 ttacttccag caggttggtg gtctggagtg taaaccggtt accggcgaga tcacctacgg
    22261 tctggaacgt ctggccatgt acattcaggg cgtagacagc gtttacgacc tggtctggag
    22321 cgacggcccg ctgggtaaaa ccacctacgg cgacgtgttc catcagaacg aagtggagca
    22381 gtccacttac aacttcgaat acgcggatgt ggacttcctg ttcacctgct ttgagcagta
    22441 cgagaaagaa gcgcagcagc tgctggcgct ggaaaatccg ctgccgctgc cagcttacga
    22501 gcgtattctg aaagccgccc acagcttcaa cctgctggat gcgcgtaaag ccatctccgt
    22561 caccgagcgt cagcgctaca ttctgcgcat tcgcaccctg accaaagcag tggcagaagc
    22621 atattacgct tcccgtgaag ccctaggctt cccgatgtgc aacaaagata agtaagaggc
    22681 ggctatgtct gagaaaactt ttctggtgga aatcggcact gaagagctgc caccaaaagc
    22741 actgcgcagc ctggcagagt cctttgctgc gaactttact gcggagctgg ataacgcagg
    22801 cctcgcacac ggcaccgttc aatggtttgc tgctccgcgt cgtctggcgc tgaaagtagc
    22861 taacctggcg gaagcgcaac cggatcgtga aatcgaaaaa cgcggcccgg cgattgccca
    22921 ggcgttcgac gctgaaggca aaccgagcaa agcggcagaa ggttgggcgc gtggttgcgg
    22981 tattaccgtt gaccaggctg agcgtctgac taccgataaa ggcgaatggc tgctgtatcg
    23041 cgcccatgtg aagggcgaaa gcactgaagc actgctgccg aatatggttg cgacttcgct
    23101 ggcgaaactg ccgattccga aactgatgcg ctggggtgca agcgacgtgc acttcgtgcg
    23161 tccggtgcac accgtgaccc tgctgctggg cgacaaagtc attcctgcaa ccattctggg
    23221 cattcagtcc gatcgcgtga ttcgcggcca ccgctttatg ggcgagccgg aattcaccat
    23281 cgataacgcc gatcagtatc cggaaattct gcgtgagcgt gggaaagtca tcgccgatta
    23341 cgaagaacgt aaggcgaaga ttaaagccga tgccgaagaa gcagcgcgta agattggcgg
    23401 taacgctgac ttaagcgaaa gcctgctgga agaagtggct tcgctggtgg agtggccggt
    23461 cgttctaacc gcgaaatttg aagagaaatt cctcgcggtg ccgtctgaag cactggttta
    23521 caccatgaaa ggtgaccaga agtacttccc ggtgtatgcg aacgacggca aactgctgcc
    23581 gaactttatc ttcgttgcca acatcgaatc gaaagatccg cagcaaatta tctctggtaa
    23641 cgagaaagtc gttcgtccac gtctggctga tgccgagttc ttcttcaaca ccgaccgtaa
    23701 aaaacgtctg gaagataacc tgccgcgcct gcaaaccgtg ctgttccagc aacagctggg
    23761 tacgctgcgt gacaagactg accgcatcca ggcgctggct ggctggatcg ccgaacagat
    23821 tggcgctgac gttaaccacg caacccgtgc gggcctgctg tctaagtgtg acctgatgac
    23881 caacatggtc ttcgagttca ccgacaccca aggcgttatg gggatgcact acgcgcgtca
    23941 cgatggcgaa gcggaagacg tagccgtcgc gctgaatgag cagtatcagc cgcgctttgc
    24001 cggtgatgac ctgccgtcca acccggtagc ttgtgcgctg gcgattgctg ataagatgga
    24061 tactctggcg ggtatcttcg gtatcggtca gcatccgaaa ggcgacaaag acccgtttgc
    24121 gctgcgtcgt gccgcacttg gcgtgctgcg aattatcgtt gagaagaacc tcaaccttga
    24181 tctgcaaacg ctgaccgaag aagcggtgcg tctgtatggc gataagctga ctaatgccaa
    24241 cgtagttgat gatgttatcg actttatgct cggtcgcttc cgcgcctggt atcaggacga
    24301 aggttatacc gttgacacca tccaggcggt actggcgcgt cgtccgactc gtccggctga
    24361 tttcgatgcc cgcatgaaag cggtatcgca cttccgtacc ctggaagcag ctgcagcact
    24421 ggcggcggcg aacaaacgtg tatctaacat cctggcgaaa tctgacgaag tgctgagcga
    24481 tcgcgtgaat gcctctactc tgaaagagcc ggaagaaatt aaactggcga tgcaggttgt
    24541 ggtgctacgt gacaagctgg agccgtactt tgcggaaggt cgttaccagg atgcgctggt
    24601 cgaactggca gagctgcgtg aaccagtcga tgccttcttc gataaagtga tggtcatggt
    24661 tgatgacaaa gaattgcgta tcaaccgtct gaccatgctg gagaaactgc gcgaactgtt
    24721 cctgcgcgtt gcggatattt cgctgttgca gtaataacgc cgttattaaa aagcctgcca
    24781 tctggcaggc tttttttatc gctaaataat acagcaacct ttaa
//
LOCUS       DAZFTN010000069        24739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFTN010000069 DAZFTN010000000
VERSION     DAZFTN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(259..2832)
                     /gene="clpB"
                     /locus_tag="ACHKV2_003093"
     CDS             complement(259..2832)
                     /gene="clpB"
                     /locus_tag="ACHKV2_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO6940936.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="ACHKV2_003094"
     CDS             complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="ACHKV2_003094"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO6940937.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="ACHKV2_003095"
     CDS             complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="ACHKV2_003095"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO6940938.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4805..5542
                     /gene="bamD"
                     /locus_tag="ACHKV2_003096"
     CDS             4805..5542
                     /gene="bamD"
                     /locus_tag="ACHKV2_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO6940939.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5813..6154
                     /gene="raiA"
                     /locus_tag="ACHKV2_003097"
     CDS             5813..6154
                     /gene="raiA"
                     /locus_tag="ACHKV2_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO6940940.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6257..6382
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6258..6305
                     /gene="pheL"
                     /locus_tag="ACHKV2_003098"
     CDS             6258..6305
                     /gene="pheL"
                     /locus_tag="ACHKV2_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO6940941.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6404..7564
                     /gene="pheA"
                     /locus_tag="ACHKV2_003099"
     CDS             6404..7564
                     /gene="pheA"
                     /locus_tag="ACHKV2_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO6940942.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="ACHKV2_003100"
     CDS             complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="ACHKV2_003100"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO6940943.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="ACHKV2_003101"
     CDS             complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="ACHKV2_003101"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO6940944.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGHLTARVA"
     gene            10019..10384
                     /gene="yfiL"
                     /locus_tag="ACHKV2_003102"
     CDS             10019..10384
                     /gene="yfiL"
                     /locus_tag="ACHKV2_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO6940945.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10534..11052
                     /gene="yfiR"
                     /locus_tag="ACHKV2_003103"
     CDS             10534..11052
                     /gene="yfiR"
                     /locus_tag="ACHKV2_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO6940946.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11042..12268
                     /gene="dgcN"
                     /locus_tag="ACHKV2_003104"
     CDS             11042..12268
                     /gene="dgcN"
                     /locus_tag="ACHKV2_003104"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO6940947.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12284..12766
                     /gene="yfiB"
                     /locus_tag="ACHKV2_003105"
     CDS             12284..12766
                     /gene="yfiB"
                     /locus_tag="ACHKV2_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6940948.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(12843..13190)
                     /gene="rplS"
                     /locus_tag="ACHKV2_003106"
     CDS             complement(12843..13190)
                     /gene="rplS"
                     /locus_tag="ACHKV2_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO6940949.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13232..13999)
                     /gene="trmD"
                     /locus_tag="ACHKV2_003107"
     CDS             complement(13232..13999)
                     /gene="trmD"
                     /locus_tag="ACHKV2_003107"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO6940950.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14030..14578)
                     /gene="rimM"
                     /locus_tag="ACHKV2_003108"
     CDS             complement(14030..14578)
                     /gene="rimM"
                     /locus_tag="ACHKV2_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO6940951.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14597..14845)
                     /gene="rpsP"
                     /locus_tag="ACHKV2_003109"
     CDS             complement(14597..14845)
                     /gene="rpsP"
                     /locus_tag="ACHKV2_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO6940952.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14982..16343)
                     /gene="ffh"
                     /locus_tag="ACHKV2_003110"
     CDS             complement(14982..16343)
                     /gene="ffh"
                     /locus_tag="ACHKV2_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO6940953.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16510..17301
                     /gene="ypjD"
                     /locus_tag="ACHKV2_003111"
     CDS             16510..17301
                     /gene="ypjD"
                     /locus_tag="ACHKV2_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO6940954.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17323..18609
                     /gene="yfjD"
                     /locus_tag="ACHKV2_003112"
     CDS             17323..18609
                     /gene="yfjD"
                     /locus_tag="ACHKV2_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO6940955.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18664..19257)
                     /gene="grpE"
                     /locus_tag="ACHKV2_003113"
     CDS             complement(18664..19257)
                     /gene="grpE"
                     /locus_tag="ACHKV2_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO6940956.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19380..20258
                     /gene="nadK"
                     /locus_tag="ACHKV2_003114"
     CDS             19380..20258
                     /gene="nadK"
                     /locus_tag="ACHKV2_003114"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO6940957.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20344..22005
                     /gene="recN"
                     /locus_tag="ACHKV2_003115"
     CDS             20344..22005
                     /gene="recN"
                     /locus_tag="ACHKV2_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO6940958.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22154..22495
                     /gene="bamE"
                     /locus_tag="ACHKV2_003116"
     CDS             22154..22495
                     /gene="bamE"
                     /locus_tag="ACHKV2_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6940959.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22557..22847)
                     /gene="yfjF"
                     /locus_tag="ACHKV2_003117"
     CDS             complement(22557..22847)
                     /gene="yfjF"
                     /locus_tag="ACHKV2_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO6940960.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22837..23274)
                     /gene="ratA"
                     /locus_tag="ACHKV2_003118"
     CDS             complement(22837..23274)
                     /gene="ratA"
                     /locus_tag="ACHKV2_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO6940961.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23445..23927
                     /gene="smpB"
                     /locus_tag="ACHKV2_003119"
     CDS             23445..23927
                     /gene="smpB"
                     /locus_tag="ACHKV2_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO6940962.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24142..24504
                     /gene="ssrA"
                     /locus_tag="ACHKV2_003120"
     tmRNA           24142..24504
                     /gene="ssrA"
                     /locus_tag="ACHKV2_003120"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6290 a         6529 c         5805 g         6115 t
ORIGIN      
        1 atacgctttc ccgctacaga gtcaagcatt tatttttgct ttctctgccg gaattctcag
       61 gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta
      121 tagggagtta ttccggcctg acaagcgaaa aatataaaaa ctttatcgtt cgctcatttt
      181 tcaggcaaaa catcttaaat atagtctttt ccgtctaact tatagacaaa aacgagcccc
      241 gaagggctcg ttttatcatt actggacggc gacaatccgg tcttcattaa cttccaggcg
      301 aataacttta cccggaacca attcaccaga cagtatttgc tgtgccaacg ggttttcgat
      361 ctgctgctgg atagcacgtt tcaaaggacg tgcaccatag actggatcgt aaccgttctc
      421 gctcagcagt ttcagcgcct cgtcagaaat gtggatttca taaccacgtt cttccagacg
      481 tttgtacaga cgtttcaact gaatctgcgc aatcgaggca atgtgctgtt cgcccagcgg
      541 atggaagacc accacttcat cgatacggtt aatgaactcc ggacggaagt tatggcttac
      601 cacaccgagc accagctctt tcatgtgcgc ataatccagt tcaccgaagc gttcctgaat
      661 cagatcggaa ccgaggttag aggtcataat gacgaccgta ttacggaagt cgaccgttct
      721 cccttgcccg tcagtcagac gcccatcatc cagtacctgc aacagaatgt tgaagacatc
      781 cggatgcgct ttttccactt catccagcag gatgacggaa tacggacgac gacgcaccgc
      841 ttcggtcagg tagccacctt cttcataacc gacatatccc ggaggcgcac caaccagacg
      901 agacaccgag tgtttctcca taaactcgga catatcgata cggaccatcg cctcgtcgct
      961 atcaaacata aagttcgcca gcgccttaca aagctctgtt ttccccacac cagttgggcc
     1021 gaggaacagg aatgaaccaa tcgggcgatt tggatccgcc agccccgcac ggctacgacg
     1081 aatagcgtta gataccgcat caaccgcttc gttctgacca attacgcgat ggtgcagttc
     1141 ttgctccata cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa ccggaatccc
     1201 cgtccaacgc gccagcactt cagcaatttc ggcgtcggtc actttattac gcaacagacg
     1261 catagttttg ccttcgagct gcgttgcggc ttccagttgc ttttccagtt ccgggatttt
     1321 gccgtattgc agttcagaca tccgcgccaa gtcacccaca cggcgagcct gttcaatagc
     1381 gattttcgcc tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg atgccttctc
     1441 tgctttccac tcttcttcta actcggagta ctgacgttct ttgtcgctca gttcttcgtt
     1501 gagcatatcc agacgttttt tactggcttc atcagactct ttcattaacg cctgttgttc
     1561 cagtttgagc tggatgatac ggcgatcgag tcggtcgagt tcttctggtt ttgagtcaat
     1621 ctgcatacga atgctggatg ctgcttcatc gatcaggtcg atggctttat ccggcagctg
     1681 acggtcagca atgtagcgat gagacaacgt cgccgctgca acaattgccg ggtcagtaat
     1741 ttgcacatgg tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg caatggtatc
     1801 ttcaacagaa ggctcggcaa caaacacttt ctggaaacga cgttccagcg cagcatcttt
     1861 ttcgatgtac tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca attcaccacg
     1921 cgccagcgcc ggtttcagca tgtttccggc gtccattgcg ccatcggctt tacccgcgcc
     1981 gaccatggta tgtaattcgt cgataaacag gatgacgttg ccttcctgtt tggcaagatc
     2041 gttaagcacg ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc cagccaccag
     2101 cgcgcccata tccagcgcca gtacccggcg gcctttcaac ccttccggca cttcgccgtt
     2161 gataatacgc tgcgccagac cttcaacgat ggcagtttta ccgacgccgg gttcaccaat
     2221 cagtaccggg ttatttttag tacgacgttg cagcacctga atggtacggc gaatttcttc
     2281 atcacgacca atcaccggat cgagtttgcc ctgttcggct cgttcggtaa ggtcgatggt
     2341 atattttttc aaagcctgac gttggtcttc agcaccttga tcgttcacgc tttcacctcc
     2401 acgcatttgt tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg ctttcaggat
     2461 gtcggccagc gtgccgcgag actcaagtgc cgccagaacg aacagttctg acgagataaa
     2521 gttatcacca cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca ccagatcctg
     2581 tgatggctgg acatcaccac cagtaccttc aacctgcggt aaacgattta atgcctgatt
     2641 gatatctgtg cgcaactggc cagcatttat gccagcggat gttaataaag gactaaccga
     2701 acccccttcc tgattcagca gggcgctcat taaatgaagt ggttcgataa attggttgtc
     2761 gtgcccaagt gcaagtgatt gggcatcggc aagagcaagc tggaatttat tagtaagacg
     2821 atccagacgc ataactcctc ccataacgga tcaaagttgc tactggagat taaatgaggt
     2881 catccctcaa ttattcaagg ttattgacca gattaatgtg aaaagaaaat cacgcgtacc
     2941 ggatcgtctt gattctttag gttatatcag ccaaatgaaa cttgccatac gaccggtggt
     3001 cttgtcgcga cgataagaga agaaagtctc attttccgta tatgtacaac ggtcgccgcc
     3061 gaaaatttgc tcaacaccca cgttcgccag acgctgccgg gcaagctgat aaatgtccgc
     3121 cagatactta tcaccatgct gaatgaaagc tgtactcgct tcggcgtcta ctgccataaa
     3181 cgcctcgcga acctccgccc ccacttcaaa cgcgcgtggg ccaattgccg gccctaacca
     3241 ggcgagaata ttttctggat tatcagcaaa acaggaaact gtctcttcca gcacgcctgc
     3301 gcacagtcca cgccagccag catgagcggc ggcgacttcc gttcccgctc gattgcaaaa
     3361 cagcacaggg aggcagtcgg cagtcatcac tgcgcaaacc ctgccgggcg tattgctata
     3421 agaggcatct gctcgttttg aggcataagg ttcgccagta agattaagca catctttgcc
     3481 gtgtacctgc tcaagccaga ccggtttaga aggcaaattg cccgcagcaa aaagtcgctt
     3541 gcgattctcc tcaacgtgat ccgggttatc gccacaatgg gcaccgaggt tgagcgagtc
     3601 atacgggggc aagctcacgc cgccgatacg agtagagcta caggccgcaa caccttttgg
     3661 cagcggccac tgcgggacaa tcagcttact cataaccagt ccacttcatc tttatgctct
     3721 tcgaaatcgg cgcgcatcac ctcaatcagc tccaccatat cttgtggaat aggcgcatgc
     3781 cattccattt cgataccgga gatcgggtga taaagacgca gcatggttgc atgaagcgcc
     3841 tggcggtcaa atttacgcag cgtggagata aacgcttccg aagcaccttt tggcgggcgc
     3901 ggacggccac cataaaccgg atcgcccacc agcggatgag tgatatgggc catatgcacg
     3961 cggatctggt gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg cacacggaag
     4021 tgttccatga tgcgatagtg agtcaccgcc ggtttgccca tcggatgcac cgccatgtga
     4081 gtacgtttgg tcgggtggcg gctgattggt tcatctactg taccgccagc ggtcatatga
     4141 ccaattgcca ccgcttcata ctcacgagta atttcacgcc tttgcaaaga ttcgactaaa
     4201 cgcgtctgag ccggaacggt ttttgccaca accatcaggc cagtggtgtc tttatccaga
     4261 cgatggacga tgcctgcacg cggtacatca gcaatgggcg gatagtaatg aagtaacgca
     4321 ttcagtaccg tgccatccgg gttacccgcg ccaggatgta ccaccagatc gcgcggttta
     4381 ttaatgacga tgatatcttc atcttcatag acgatatcca gcgggatatc ctgcggttca
     4441 aaacgcgctt cttcttcaat ctcagcgttg atggcaacct gctcgccacc caatactttt
     4501 tctttcggct tatcacaaag tttgccgtta accagcacgc gctggtcgag gatccattct
     4561 tttattcgcg aacgtgaata atccgggaac atttcggcca aagcctgatc taagcgttga
     4621 ccgagttggt tttcggacac cgttgcagtg agctgtactc gttgtgccat atatactgct
     4681 tcttcgttta tcgttggggt tttacggctt tgccgtttaa tatagtgtgc tattgtagct
     4741 ggtcttaacc gggagcagga acagagaatc tcccgtatta cattttgagg aaagtcaaaa
     4801 cgtcatgacg cgcatgaaat atctggtggc agccgccaca ctaagcctgt ttttggcggg
     4861 ttgctcgggg tcaaaggaag aagtacctga taatccgcca aatgaaattt acgcgactgc
     4921 acaacaaaag ctgcaggacg gtaactggag acaggcaata acgcaactgg aagcgttaga
     4981 taatcgctat ccgtttggtc cgtattcgca gcaggtgcag ctggatctca tctacgccta
     5041 ctataaaaac gccgatttgc cgttagcgca ggctgccatc gatcgtttta ttcgccttaa
     5101 cccgacccat ccgaatatcg attatgtcat gtacatgcgt ggcctgacca atatggcgct
     5161 ggatgacagt gcgctgcaag ggttctttgg cgttgaccgt agcgatcgcg atcctcaaca
     5221 tgcacgagct gcgtttagtg acttttccaa actggtgcgc ggctatccaa acagtcagta
     5281 caccaccgat gccaccaaac gtctggtatt cctgaaagat cgtctggcga aatatgaata
     5341 ctccgtggcc gagtactata cagaacgtgg cgcatgggtt gccgtcgtta accgcgtaga
     5401 aggcatgttg cgcgactacc cggataccca ggctacgcgt gatgcgctgc cgctgatgga
     5461 aaatgcatac cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga aaatcatcgc
     5521 cgcaaacagc agcaatacat aacagaaacc tgaaacacaa aacggcagcc cttgagctgc
     5581 cgttttttta ttctgtcagt tgtgaaactg aagcgattta gtcactatcg atctcatcaa
     5641 atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac
     5701 aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca
     5761 tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat
     5821 gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg
     5881 tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa
     5941 agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc
     6001 cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg
     6061 gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga
     6121 cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac
     6181 gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactaaagt
     6241 cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt tttaccttcc
     6301 cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agaagaacaa taaggcctcc
     6361 caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaaccc
     6421 gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc
     6481 agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc atcgcccggt
     6541 acgtgatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca
     6601 ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag attccgtatt
     6661 aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact cagcacgcat
     6721 cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg
     6781 tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt
     6841 ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat
     6901 aaatgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg agatgacgtt
     6961 aactatcgac cattgtttgt tagtctccgg cacgactgat ttatccacca tcaatacggt
     7021 ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg
     7081 gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc
     7141 accgcatgtt gctgcgttag gaagcgaagc tggcggcact ttgtacggtt tgcaggtact
     7201 ggagcggatt gaagcgaatc agcgacaaaa cttcacccga tttgtggtgt tggcgcgtaa
     7261 agccattaac gtgtctgatc aggttccggc gaaaacgacg ttgttaatgg cgaccgggca
     7321 acaagccggt gcgctggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac
     7381 ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt atctggatat
     7441 tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac
     7501 ccgttcaatg aaggtattgg gctgttaccc tagtgagaac gtagtgcctg ttgatccaac
     7561 ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac tggcgattgt
     7621 cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga
     7681 accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca
     7741 actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg
     7801 acgacataat aatgtcggca taaagctgcg gatcctgagc gaacagtcgc ccgaccatcg
     7861 ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt
     7921 cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagtgcc tgaataaacg
     7981 ccatattctg atcgtgctcg acggcgctaa tacgatgcaa ccgagcgccc cagacctgaa
     8041 tttgctccag aaaccattgg tatgcttccg gtttacgtcc atcacaccag accacaactt
     8101 gctttgccag gctaccgctg tccggaccga acattgggtg taaccccagt accgggccat
     8161 cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga tcaaccagaa
     8221 tacaatcttt cggtaaaggc ggtaatttgc caataacttg ctcagtaacg tggattggca
     8281 cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt
     8341 gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac agacgtccca
     8401 tctgaccgcc gccgccaacg ataactaccg ggcgtaacgc aggacaaagc gttttaaatc
     8461 ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca
     8521 gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct
     8581 ctcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa
     8641 ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat
     8701 cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg agccgtcaga
     8761 tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccagctaatg
     8821 caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc ggaagactga
     8881 ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt gccatctttg
     8941 atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt attggaatta
     9001 ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca
     9061 cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag gatgacatgc
     9121 ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt aatgccaaca
     9181 aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg
     9241 gtgccgtttt taaagccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc
     9301 gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg
     9361 ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc
     9421 aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg
     9481 ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc
     9541 agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga
     9601 gcagtttccg gatcatgaat ggaacaaggg ccacatacta ccagcagacg aggatcgcgc
     9661 ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg
     9721 cttaatggaa aagcggcctt cagttgtgcc ggagtcatta aaacctgttc gtcggtaata
     9781 tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat
     9841 agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata
     9901 tttcgtacat ttttctttac ataagcaatt tattcgcgct ttcaaccctt gattctgtat
     9961 agataaattt acaccacctt tgaaaacaat ccgctatgct ttgaaaaagg agaaagaaat
    10021 gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgcc agattgatcc
    10081 ttatactcac gcgccaacct tgaccagcac cgactggtat gatgttggta tggaagatgc
    10141 gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg cggatcgcgg
    10201 tctatacctt aaaggatatg ccgaaggaca aaagaaaact tgccagaccg attttactta
    10261 tgcccgagga ctttccggta aaagctttcc tgccagctgc aacaatgttg aaaatgccag
    10321 ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcgcaa tacgattaaa
    10381 ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattatgt tttatttctc
    10441 acaatgatga cgaccgcatg aaataatgat attttaatga gcattctgaa aaaatatcga
    10501 tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg actgttctta
    10561 ctgctgattc tcctgctgac gggagcacca atactcgctc aggagccttc tgacgttgcg
    10621 aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc tgcgctatca
    10681 gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct acaagagaat
    10741 gccgcaacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga ggcgatgatt
    10801 tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat ggaattaacc
    10861 gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga gtgcattatt
    10921 ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt aaacctggat
    10981 gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc acggaagaaa
    11041 aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag cattacgcaa
    11101 catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc tttccgtgac
    11161 ctcggtgctg cccctgaaac agtacgcgca aaaaaacctg gcactgacag cagcaacaat
    11221 gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa ctgaaacact
    11281 ggcagcgctg ggccagcaag ggcaattttc aactgcagaa gtacgtgata agcagcaaaa
    11341 tattctggca tcctggcatt acacccgtaa ggatccaggc gatactttca gcaatttcat
    11401 aagccactgg ctcttccctg cccccatcat tcagccgatt cgtcacaatg gtgaaaccat
    11461 tggcgaagta cgcttaaccg ctcgcgacag ttcaatcagc cattttatct ggttttcgct
    11521 cgccgtactg accggttgta ttctgctggc atcaggaatc gcaattaccc tcacccgcca
    11581 tttgcacaat ggcctggtgg aagcactgaa aaatatcacc gatgtcgtac atgatgtgcg
    11641 ttccaaccgc aatttttccc gacgagtttc ggaagaacgt atcgctgagt ttcaccgctt
    11701 cgctctcgac ttcaacagtc tgctggatga aatggaagag tggcagcttc gtttacaggc
    11761 caaaaatgcg cagcttctac gtaccgcgct acatgaccca ttaaccgggc tggctaaccg
    11821 cgcagcgttt cgtagcggca tcaatacgtt gatgaacaat tccgctgccc gaaaaacgtc
    11881 ggcgttacta tttcttgatg gcgataattt caaatatatc aatgatacct ggggtcatgc
    11941 gacgggcgat agagtcttga ttgaaatcgc aaaacggtta gctgaatttg gcgggctgcg
    12001 acataaagca taccgcctgg gcggcgatga attcgctatg gtgctctatg gtgtacagtc
    12061 ggaatctgaa gtgcagcaga tatgctcagc actgacacaa atctttaatc tcccgtttga
    12121 tcttcataat ggccatcaga ccaccatgac attaagcatt ggttacgcaa tgaccattgc
    12181 gcacgcttct gcggaaaaat tacaagagct ggccgatcac aatatgtatc aggccaaaca
    12241 ccagcgtgcc gaaaagctgg tgagataaca aggatatatc gatatgataa agcacctggt
    12301 agcacccctg attttcacct cactaatact gactggctgc cagtcccctc agggaaagtt
    12361 tactcctgag caagtcgccg ctatgcaatc ttatggattt actgaatccg ccggcgactg
    12421 gtcgctgggc ttatcagatg ccattctgtt cgcaaaaaat gactacaaat tgctcccgga
    12481 aagccagcaa cagatccaaa ccatggcagc caaattggcc tcgacagggc taacacatgc
    12541 ccgtatggat ggacacaccg ataactatgg tgaagacagt tacaacgaag gcttatcatt
    12601 gaaacgggcg aatgtcgtgg ccgatgcatg ggctatgggt ggacaaattc cacgcagcaa
    12661 tctcaccaca cagggtttag gaaaaaaata tcccatagcc agtaacaaga ccgcccaggg
    12721 ccgcgccgag aaccgccgcg tcgcagtggt gattactccc ccttaaaaca cgagcaaaca
    12781 gataaaaaaa gggccagcca attggccagc ccttcttaac aggatgtcgc ttaagcgaaa
    12841 tcttagttaa gacgctcttt gatacgagca gccttaccag tacgctcacg caggtagtac
    12901 agtttagctt tacgaacagc accacgacgt ttgacagaaa tgctgtcaac taccggagag
    12961 tgagtctgga agacacgctc aacgccttcg ccgttggaaa ttttacgaac agtgaatgca
    13021 gagtgcagac cgcggttacg aatagcgata accacgccct cgaatgcctg cagacgtttt
    13081 ttggaacctt caacaaccca tactttcact tccacggtat cacccggacg gaaggaaggt
    13141 acgtcctgct tcatctgctc ttgttcaagt tgcttaataa tgttgctcat aatttaatct
    13201 cttatcctgg gtaaactgat atctcggggg cttacgccat cccatcatgt ttatgttgct
    13261 gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc ttgctcttca gtcagagcca
    13321 ggttttccag aagttcaggt cttctaagcc aggtacggcc cagcgactgt ttcaaacgcc
    13381 agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg cggaacttcc atcccttcta
    13441 acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc ttcagcaaag gaatcttccg
    13501 ttgccgaggc ttcatgtccc agtactcccg gaataaaccg ggaaacggag tcaatcagcg
    13561 tcattgctgg taactcacca ccactgagaa cgtaatcgcc gattgaccat tcttcgtcaa
    13621 tttcggtttg gatcacgcgc tcatctatac cttcgtagcg accgcacacc agaatcaatt
    13681 tttgattcgt tgccagttcg ctgacgcccg cttgatcaag cttgcgtccc tgtggtgaaa
    13741 gataaatcac ctttgcgcct tcacccgccg cggcttttgc tgcatgaatg gcgtcccgca
    13801 agggttgcac catcattaac atccccggtc cgccgccgta aggacgatcg tccacggtac
    13861 ggtgccggtc atgcgtgaaa tcgcgaggac tccagctctg gatgctcagc aggccatttt
    13921 taactgcccg gccagttacc ccgtaatcgg taattgcgcg gaacatttca ggaaacaggc
    13981 taattatgcc aatccacata gcgccgtctt ttaccgttta tccggtggtt taaaaaccag
    14041 gatcccaatc tacttcgatt gaacgagtag tgagatcgac tttcttgata acctgcccat
    14101 cgaggaacgg tacgagacgt tccttgatac caaacgcatc tttcaggttt gccttaatga
    14161 cgagaacgtc attggatccg gtttccatca tatcgacgac tttaccgaga tcgtagcctt
    14221 cagtggttac tacctggcag cccatcaggt ctttccagta gtagtcaccc tcttcaagct
    14281 gaggcagctg cgatgaatcc acgacaattt cacaattcgt cagcaggttc gccgcatcac
    14341 gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt gtggtgcttc cagctttcca
    14401 gctggacttg ctgccactga cccgccttct ggataaacca gggctgatag tcaaaaatgc
    14461 tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc acgaataccg taagacgaac
    14521 ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc ggtgagttgt ttgctcatca
    14581 tgaccaccgt gacagattaa gctgctttgt ttacttcttt gatcagcgca gcaacgcgat
    14641 cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg atccagatcc aggcgagtgc
    14701 cttcttcttt ttcgctagcg attgggttga agaaaccaac gcgctcgatg aagcgaccgt
    14761 tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa cggacgcttt ttagcgccgt
    14821 gacgtgctaa acgaatagtt accataacat cctcttgtgt gaataaaaca accggacccc
    14881 atcgaggaac ggagtccggt gtcatattaa aagcccgaaa attttactga tttctggaga
    14941 aaatgcaatc agcagttaat aactctgtcg cataaggtca tttagcgacc aggaaatccc
    15001 ggcggcatca tccccttcat gcttctcatc atcttcgcca ttccgccctt cttcattttc
    15061 ttcatcatgc gctgcatgtc gtcgaactgt ttcagaagac ggttaacgtc ctgcacctgc
    15121 ataccgcaac cggcagcaat acggcgttta cgcgaacctt tgatgatttc tggcttagcg
    15181 cgctctttca tcgtcatcga gttgatgatg gcttccatac gcaccagcac tttatcgtcc
    15241 atctgtgact tgacgttatc cgggatctgc cccatgcccg gcagtttgcc catcaggctg
    15301 gccatgccgc ccatattttt catctggcgc agttgctcaa ggaagtcgtt gagatcgaag
    15361 ccgtcacctt ttttcagctt gctggctaat ttctctgcct gcgcgcggtc aactttgctt
    15421 tcgatatctt cgatcagcga cagtacgtcg cccatgccga gaatacgcga cgcgatgcgg
    15481 tccggatgga acggctccag cgcctcagtt ttctcgccaa caccgaggaa cttgatcggt
    15541 ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc gggcatcgcc gtccactttg
    15601 gtcaacacta cgccggtaag cggtaacgct tcattgaatg cttttgccgt attggccgca
    15661 tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa ccgggttaat cgacgcatgg
    15721 acttgtttga tctcgtccat catcgcttcg tcaacgtgca gacgaccagc ggtatccacc
    15781 agcagcacgt cgtagaattt cagtttggct tctttcagcg ccgcgttaac aatatcaacc
    15841 ggcttctgac caacatcaga agggaagaaa tcaacgccca cctgctctgc cagcgtctca
    15901 agctgtttga ttgccgccgg gcgatagacg tcagcagaaa ccaccagcac tttcttcttg
    15961 tgcttctcgc gcaggaactt accgagttta ccaacgctgg ttgttttacc ggcaccttgc
    16021 aggcctgcca tcagcacgac cgcaggcggt tgcgcagcca gattcagggt ctggttctct
    16081 tcgcccatcg ccgcaaccag ttcgttacgg acgattttga cgaactcctg ccccggcgtc
    16141 aggctcttat taacttcatg accaaccgct ttctctttta cgcgattgat aaactcacgc
    16201 actaccggca gagcgacgtc cgcctccagc agcgccatgc gcacttcgcg cagcgtatct
    16261 tttacgttgt cttcagtgag gcgtccacgg ccactgatat tgcgcagcgt gcgcgacaaa
    16321 cgatcggtta aattatcaaa cattgtctct cgcctggggt ggaaacggtt ggccgcaatc
    16381 gcgacacatc atcagtattt tgccgcagta taacatgaag gcgtctttgt tgttatgcaa
    16441 cggttggagc agcgttcacc tgacgctata ctgcttctct ttcttattgc tcaaactgtc
    16501 gacatcacta tgcccgtttt tgctctgctc gcgcttgtcg cctactccgt cagtcttgcg
    16561 ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc ggcgcatggc tattatttct
    16621 gcggtcattg cgctggtctg ccacgcaatc gctctggaag cccgcatcct gcccgacggt
    16681 gatagcggac aaaacctcag cctgctgaac gttggttcat tggtcagttt gatgatctgt
    16741 acggtaatga ccattgtggc ttctcgcaat cgtggctggt tgctgctacc cattgtctat
    16801 gcctttgcgc ttatcaacct ggcgctggca accttcatgc ccaatgaata catcacccat
    16861 ctggaagcta cgcctgggat gctggtgcac attggcttat cgctcttttc ctatgccacg
    16921 ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga ttgattacca actgaagaac
    16981 aagaagctgg cgtttaacca ggaaatgccg ccattgatga gtatcgagcg taaaatgttc
    17041 cacatcacgc agattggcgt ggtgctgcta acgctcacgc tttgcactgg cctgttctac
    17101 atgcacaact tatttagcat ggaaaatatc gacaaggctg tgctctctat cgtggcgtgg
    17161 tttgtctata ttgtgctgct atggggacat tatcatgaag gatggcgtgg acgtcgcgtc
    17221 gtctggttta acgttgcggg tgcagtcatt ctgacactgg cctacttcgg cagccgaatt
    17281 gtccagcagt taatcagcta aacccagaaa aggagtttcc ccctggaaca catttctact
    17341 actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt ttccgggtcc
    17401 gaaaccggaa tgatgaccct caaccgctat cgtctgcgac atatggcgaa acagggtaat
    17461 cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat aagcctggtg
    17521 ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat tgttgggatg
    17581 cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt tgtcgtactg
    17641 gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa agtcgcttat
    17701 ccgagtagtt ttctgctggc tcccctgcaa attttgatga tgccgctggt ctggttgctg
    17761 aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat cgtggttagc
    17821 ggctctttga gcaaagaaga gttgcgcact atcgtgcacg aatcgcgctc acaaatttcc
    17881 cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac cgttgatgac
    17941 atcatggtgc cgcgcagtga aattatcggt attgatatca acgatgactg gaaatctatt
    18001 ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tctaccgtga ttcgctggac
    18061 gacgccatca gtatgctgcg agtacgtgaa gcttggcggt tgatgtcgga gaaaaaagag
    18121 ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc ggaaggtacg
    18181 ccgctcagca cgcagttggt gaagtttcag cgcaacaaaa agaaagtcgg cctggtcgtc
    18241 aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga agagattgtc
    18301 ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aagtcacgcc gcaaaacgac
    18361 ggttcggtga ttattgatgg cactgccaac gtgcgggaaa tcaacaaagc ctttaactgg
    18421 catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc actggaagag
    18481 atccctgtcg taggcacccg cgtgcgtatt ggcgagtacg atatcgatat tctcgacgta
    18541 caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg cgagagtgtg
    18601 gcggagtaac gaaaacggcc cggcattcgc atgcagggcc gtgaattatt acgaaaacag
    18661 aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac gaccattcag
    18721 cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat cagattccac
    18781 cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg cgatcacttc
    18841 aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa tgccttcaac
    18901 catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat ccaggctatc
    18961 aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg ctttttcaat
    19021 atccagttca gtacgacgac gcaggttttc catttcggct tttacacgca aaatgccgtc
    19081 acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt tttcatcgcg
    19141 cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt cgtgctgatc
    19201 catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac tactcatgaa
    19261 tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg gatcagtttc
    19321 agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac ctcggaaaaa
    19381 tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct actgcactga
    19441 caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc atcgttgagc
    19501 aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc gcggagattg
    19561 ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg ggcgcgacgc
    19621 gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac ctgggtttcc
    19681 tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg gaaggtcact
    19741 acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat tgccaaaagc
    19801 gcatcagcac cgcgataaat gaagtggtac ttcatccagg caaagtggcg catatgatag
    19861 agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat gggctaatta
    19921 tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct attctgaccc
    19981 cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca gcacgaccac
    20041 tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt aacgacctgg
    20101 aaatcagttg cgacagccag atagcactac cgattcagga aggtgaagat gtcctgattc
    20161 gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat ttcaacacat
    20221 taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc ctctttactg
    20281 tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata caggaaaacg
    20341 actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct tgagattgat
    20401 tttcatagcg gcatgaccgt aataactggc gaaaccggtg cgggtaaatc tattgcaata
    20461 gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg taccggcgct
    20521 gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc cctgcgctgg
    20581 ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt gatcagcagc
    20641 gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca actgcgcgaa
    20701 ctgggtcagt tgctgattca gatccatggt cagcacgctc atcagttact caccaaaccc
    20761 gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact ccaggaaatg
    20821 accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca tcaacagtta
    20881 agtcaggaac gcgctgcccg tgcggaactg ctgcaatacc aattaaaaga acttaacgaa
    20941 tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg tctggcgaac
    21001 agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga cggtgaagac
    21061 gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt gattggcatg
    21121 gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca gattgttgaa
    21181 gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa ccgactgttt
    21241 gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca cgtcagccct
    21301 gagacattgc cacagtatta ccagtcgcta ctggaagaac agcagcaact ggacgatcag
    21361 gccgactcac aagaaacgct cgcactggcg gtaacgaaac atcatcagca ggcactggaa
    21421 acggcgcgcg cattacacca gcaacgccag cattatgcag aagaacttgc acagctgatc
    21481 accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga tgtcaaattt
    21541 gacgagcatc acctgggcgc tgacggcgcc gatcgtattg agtttcgggt aaccaccaac
    21601 ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt gtcccgcatc
    21661 gcattggcaa ttcaggtcat cacggcgcgt aaaatggaaa ccccggcact gatttttgat
    21721 gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact gctgcgtcaa
    21781 ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc gggatgtggt
    21841 catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac gcatatgcaa
    21901 tccctgaata aaaaaacgcg gttacaagag ctggcgcgcc tgcttggtgg cagtgaagtc
    21961 acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcgg cgtaaacttt tttcctgctt
    22021 cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt ttaaagtgat
    22081 gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg gcccgaaaag
    22141 gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac tattgatgtt
    22201 gaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca accaggggaa
    22261 ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac aacaagttgc
    22321 gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct ggttctatgt
    22381 cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc taacctttaa
    22441 cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta actaataata
    22501 aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc tgtccgttat
    22561 ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc aatgagaggg
    22621 cgataaatct ccacccgatc gccatcatgc acactatcgc ttaattttgc cggacggctg
    22681 taaatgccga ctttgttttc agttaaatcg atatcggtac gcaattccag caagccactg
    22741 gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcacgcgctg caggtactgc
    22801 ttcttaggta gcgcataagc cacctcaacg gcaattttac ctggcactgt aaacctcttt
    22861 cgcacgaacc gtaaaagcct ggaccatatt agacgccagc tctttaaaca cgcgaccaaa
    22921 agcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga tacgacacgc
    22981 ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg gcccatccac
    23041 cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa acgttttgct
    23101 gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg actccagaat
    23161 ccgacttccg gtacaacccg gcaaaaactg aggataagac tgaacgtcat tcactaactg
    23221 atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag gcataacaat
    23281 ttccatcaac aaaaatccaa caaataatat catttatcct gcagtaaaaa aaacgctatc
    23341 ccggcgctgg gtaacatccg gttcatgcta agatagagcc ttgtcccccg caggattgat
    23401 atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg aagaaaaaag
    23461 cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac gaatacttta
    23521 ttgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa tccctgcgcg
    23581 caggaaaagc caatattagc gacagctatg tccttctgcg cgacggagaa gcgttcctgt
    23641 ttggcgcaaa catcacgcca atggctgtgg cttctacgca tgtggtgtgc gatcctaccc
    23701 gtacccgcaa gttacttctc aaccagcgcg aacttgattc gttgtacggt cgcgtcaatc
    23761 gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg tgcaaagtga
    23821 aaatcggcgt cgccaaaggt aagaaacagc acgataaacg ttcagatatc aaagaacgcg
    23881 aatggcaggt ggataaagcg cgtatcatga aaaacgccca ccgttaaacc tgcactccaa
    23941 ttcttgacct gttcctcacc gcgcctccct ctccggcggc gcgaatgaac atcttattgg
    24001 ctatcacatc cgacaaaaat gttgccatcc cattgcttaa tcgaataaaa atcaggctac
    24061 atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatagcgct cataaatctg
    24121 gtatacttgc cgttacacat tggggctgat tctggattcg acgggatttg cgaaacccaa
    24181 ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc gcaaacgacg
    24241 aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta gcctccgctc
    24301 ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc cctgcctggg
    24361 gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc cgcagctggc
    24421 aagcgaatgt aaagactgac taagcatgta gtaccgagga cgtaggaatt tcggacgcgg
    24481 gttcaactcc cgccagctcc accactttaa gaaggactac aaccggacag tagcaataaa
    24541 tacagccact tacggacaca agccagacag tagtcggact gaaaaaagac aaaaatatac
    24601 acgtgaaatg cacgtgcact tctaaaaaac cccagttatc accgactggg gtttttcaat
    24661 ttgtaactaa gggtaacaac ccccccgttt ttttcatctc actcaccttg atactgttta
    24721 tttgtacagt taaaaataa
//
LOCUS       DAZFTN010000070        23967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFTN010000070 DAZFTN010000000
VERSION     DAZFTN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(193..4074)
                     /gene="entF"
                     /locus_tag="ACHKV2_003121"
     CDS             complement(193..4074)
                     /gene="entF"
                     /locus_tag="ACHKV2_003121"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO6940963.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(4071..4289)
                     /locus_tag="ACHKV2_003122"
     CDS             complement(4071..4289)
                     /locus_tag="ACHKV2_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO6940964.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4292..5494)
                     /gene="fes"
                     /locus_tag="ACHKV2_003123"
     CDS             complement(4292..5494)
                     /gene="fes"
                     /locus_tag="ACHKV2_003123"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO6940965.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5737..7977
                     /gene="fepA"
                     /locus_tag="ACHKV2_003124"
     CDS             5737..7977
                     /gene="fepA"
                     /locus_tag="ACHKV2_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO6940966.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            8152..8772
                     /gene="entD"
                     /locus_tag="ACHKV2_003125"
     CDS             8152..8772
                     /gene="entD"
                     /locus_tag="ACHKV2_003125"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO6940967.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8894..9046)
                     /gene="hokE"
                     /locus_tag="ACHKV2_003126"
     CDS             complement(8894..9046)
                     /gene="hokE"
                     /locus_tag="ACHKV2_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6940968.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9397..9549)
                     /gene="hokE"
                     /locus_tag="ACHKV2_003127"
     CDS             complement(9397..9549)
                     /gene="hokE"
                     /locus_tag="ACHKV2_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6940969.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9518..9785
                     /locus_tag="ACHKV2_003128"
                     /pseudo
     CDS             9518..9785
                     /locus_tag="ACHKV2_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9988..11106
                     /locus_tag="ACHKV2_003129"
     CDS             9988..11106
                     /locus_tag="ACHKV2_003129"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO6940970.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            11172..11420
                     /locus_tag="ACHKV2_003130"
     CDS             11172..11420
                     /locus_tag="ACHKV2_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO6940971.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11485..11853
                     /locus_tag="ACHKV2_003131"
     CDS             11485..11853
                     /locus_tag="ACHKV2_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6940972.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11947..12600
                     /gene="nfsB"
                     /locus_tag="ACHKV2_003132"
     CDS             11947..12600
                     /gene="nfsB"
                     /locus_tag="ACHKV2_003132"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO6940973.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12708..13955
                     /gene="ybdG"
                     /locus_tag="ACHKV2_003133"
     CDS             12708..13955
                     /gene="ybdG"
                     /locus_tag="ACHKV2_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO6940974.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(14023..15399)
                     /gene="pheP"
                     /locus_tag="ACHKV2_003134"
     CDS             complement(14023..15399)
                     /gene="pheP"
                     /locus_tag="ACHKV2_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO6940975.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15501..18638)
                     /gene="cusA"
                     /locus_tag="ACHKV2_003135"
     CDS             complement(15501..18638)
                     /gene="cusA"
                     /locus_tag="ACHKV2_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO6940976.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18650..19873)
                     /gene="cusB"
                     /locus_tag="ACHKV2_003136"
     CDS             complement(18650..19873)
                     /gene="cusB"
                     /locus_tag="ACHKV2_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO6940977.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19889..20221)
                     /gene="cusF"
                     /locus_tag="ACHKV2_003137"
     CDS             complement(19889..20221)
                     /gene="cusF"
                     /locus_tag="ACHKV2_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO6940978.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20245..21627)
                     /gene="cusC"
                     /locus_tag="ACHKV2_003138"
     CDS             complement(20245..21627)
                     /gene="cusC"
                     /locus_tag="ACHKV2_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO6940979.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21784..22467
                     /gene="cusR"
                     /locus_tag="ACHKV2_003139"
     CDS             21784..22467
                     /gene="cusR"
                     /locus_tag="ACHKV2_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO6940980.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22457..23905
                     /gene="cusS"
                     /locus_tag="ACHKV2_003140"
     CDS             22457..23905
                     /gene="cusS"
                     /locus_tag="ACHKV2_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO6940981.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT         5775 a         6530 c         6075 g         5587 t
ORIGIN      
        1 caataggggg aaatctccac ggcgctcact ttaccagttg gtgagaatta tccgaagctg
       61 aagtttgtaa attcattcca ctgaatgcaa cattcactct atttaagaaa aataaccagc
      121 aaacttataa attaaataaa tgataaaccc ataattattt cctgcaatta tgggtttata
      181 gataatgtta atttatctgt ttagcgttgc gcgaataatc ggcccaattt tttcaaatgc
      241 ccctggagag ataatatcca catgcgcaca atcctgacga tagatatcca gctccgcaat
      301 ccacggcgac caggcgcgtt cggggctcat accttcctga agcgtacgtt cagcaacaaa
      361 cagcgtcgct tttccgtcaa acggtacgct atgagcagtc gtcagcaggc gcacagcatc
      421 agcgtagttg ccttcaatgg tggtaaacaa ctccgttgaa gtacttccct gctgtgccgc
      481 caggaaggcc tcgcgctcgc ggttaatctc cgccagcact tccgggtcca gaccattagc
      541 ttctttttcc tgccagtttt gcgtttctgg cggccaggta tccagcaagc caagaaatgc
      601 cacctgttcg ccacgggcac gtagtcgcgc cgcaatgccc tgcgccagtg taccgccaag
      661 ggaatacccc agcaggtaat aagggccgtg cggttgttgt tcaagtaacg ttgccagatg
      721 cgcttcgcag acttcatcca ggttcgtcgc tgtctgcatg gggccatgag ggcgcggcga
      781 ctgaatgccg ataatcgacc atagtggatc gagataacgc gagagcacgc taaactgcca
      841 ggcaaaaccg gatgccggat ggaaacaaaa cagcgtcggg ccattacctt cacgcaacgg
      901 cagaatggtt tcgaatccca tgcgccggga gctgtcctct tcaccatcaa taatcgttgc
      961 cagtttggcg acggttgacg cgaccatcac ctgccccggc gtcacctgac gggcaaactg
     1021 ccgacttaac tgcgctgcca gtttcattgc cagtagcgaa tgaccgccaa gcgcgaagaa
     1081 atcagcatcg gcatcctgca cgtcacaacc cagcaacgac gcgaatgccg cagcgataat
     1141 cgtttcactg cccgctttcg gcgcacgccc cggcgtttgt gccttaagtt caggcaacgg
     1201 taaggctttg cgatccagct tgccgttggc gctaagaggt aactgtggaa gttgcagcag
     1261 aacgaccggc gccatatgcg gcggcaatgt ttcgcgaagc tgtgcctgta atgcgctggt
     1321 atccaacggc agacctgatt gcgacaccag atagcccacc aactgacgcg catcaccacc
     1381 ggtggctgcc gcctggttaa tcacacaggc gtgggtaacg gcttgttcga catccggcag
     1441 cgcctgcatc acgcgatcga tttcgcccag ttcgatacgc tgcccgcgaa tttttagctg
     1501 atcgtcactg cgcccgaggt actccaccgc gccgttatcc agccagcggg caacgtctcc
     1561 ggtacggtac atccgttcac cagggacaaa aggatcggca atgaagcggc tggcggtcag
     1621 atcgggtcgt ccaagatacc cctgcgccag ttgaataccg gtgagataga gatctcccgc
     1681 cacacccggc ggcaccggat gcatcatcgc atcgagaatg cgcaagcccg tattccacac
     1741 cggataacca atcggcacac tgctgccgcg cacctgtgcc agttcctcgc caaaagccgg
     1801 ataccaactc acatctaccg ccgcttccgt cgggccatat agattatgca acggcgcgcc
     1861 cgttaactgt tgccattcgc ggcataaatc agccggtaag gcctcaccac tacagaaaac
     1921 ctgtttcaac gtcgcgcaat tctggcgagc ggtttgcggc gtcagcgatg caacaaatgc
     1981 cgccagcatc gacggcacaa agtgcgtggt cgttacgcca tattcggcaa agaattgctg
     2041 catagcgagc gggtcgcggt gcgcttccgg ttcagccatc accagtttag ccccggcaat
     2101 aaacggccag aaaaactccc acaccgagac atcaaaactg cacggcgttt tttgggcaac
     2161 gacatcttca cctgtaagtg gataatgatt ttgcatccac aacaggcggt taacgatagc
     2221 cgtctgcccg accattaccc ctttcggcct gccggtggag ccagaggtaa agatgatata
     2281 agccgtgtga tgcggttgtg aaagttgcag cggcgcactg ccctgcggtg taagcggggc
     2341 gttatagcaa aggttggtta aatctggaac atcggcaaag cgcggcagtt gatcgtcggt
     2401 ggtgattaac agcgacggac gcgcatcttc cagcatcatt ttcaggcgat cgtccggata
     2461 accggtatcc agcggtaacc aggccgcacc tgcttcaaca atcgcatgta gcgccagggt
     2521 caaaaagacc gagcgcggta atgccaccgc cacgctgtcc cctggtttaa cgccgtgctc
     2581 acgcagcaga ttcgccagcg ccaccacctg ctcgcgcatt tcccgatagc tgaactggta
     2641 acgcgcatct gccagcgccg gagcatccgg tgtttttgcc gcttgttctg ccaccagcgc
     2701 gctaagcgtg gtttctggaa tctcaacctg agtggcgttg atctgcgcca gctgcgcata
     2761 ctcacctggc agcataatat caacatcgcc gcacaacaga gccggatccg cagcgaattg
     2821 ggcgatcagc attttcaggc gttcagcatg ctggattaac gttggctcat cgtaatgctg
     2881 tttattggcg aggatctcaa tactcaaatc accgtgttca tccgggaaca gggccagttc
     2941 aaggtcatta accggaccgg ttgccagggt atgggtttgc gcctgaacac caggaatatc
     3001 cagttggtaa tcaaatacct tgatattgag taccggacca aacagcggtt catcacctgc
     3061 cgctcgcccg ctgtcacgga caatttgttc ggcatcgtaa cgttgatgac gacgcatttt
     3121 tttcagttgt gctgccagtc gggttgccag ttccggcagc gtttcttgtg ccgcaatgtg
     3181 aatacccaac ggtaaaacgt tgagcacggg tccggtagcc gtcagcgccg ccgagcccag
     3241 tcgacgcata aagataaatc cggcggcgta gtccatgcga ttgcacaatc gccccagcca
     3301 aaaggctgcc agcgcaaggg ctaaatcggt acgctgcaca cctgaaagtt gcgtagccag
     3361 ctggcggaat tccccgtcgg taaattccag tttcaggcgt agaatatctg ccgaagcgct
     3421 gcgccccgct aaaggtgccg gagaaagtga cgcgggcggc ggcagttgac gacgctgttc
     3481 cgcccagaat gccgcatcac gctgccaggc ttcgctttcg cggtattgct ggtactcttc
     3541 cactacatca gcgaaaggcg taaacggcga agcaggcgtt tgttcgccac gcagcaatgc
     3601 gcagtaaatg ttggcgatct gccgagtaat ggccgggaaa ctgaagccat cgaccagcaa
     3661 atggtgataa cgctgatacc agtaccagcg gttatctgcc acctgtatca gctggtgaaa
     3721 gaccagtggt ttaccgctat cgacgcgcaa atcttgttgc aaatccgcct gcattaatgc
     3781 ctgcgcagta ccgtgcggat caatattggt tcgcaggtcg ataatttctg gcagttcgaa
     3841 tatcagcgca tcatcgaccc attgccagac ttcgccgtta tcttccgtaa aacgcatccg
     3901 cagcgtatct gcttgcgcta atccggcaac caccgcgcgg gccagtaatg gcgcatcaac
     3961 ctctccggtt aactcaacgt aatgcgccac gctccaggcg gagggtaatt ctgacagttt
     4021 ttctgccatc cagatgccgg gctgtgcggc gaccaaaggt aaatgctggc tcattgtgcc
     4081 tcctgcaact gggtgaaatt cgccggtgtc agagtacgcc agtggacttc cagccactgc
     4141 tggcaggact cttgtgactg cggctgacac acaatgtccc agcctgccgg taaggcgcat
     4201 tgttgcggcc acagactgaa ttgcccctgc gcattgcgca aaatgtaaaa cactccctgt
     4261 ggatcatcga agggattact gaatgccata ttcaactcct gtcatggaaa agtggttgcc
     4321 agaggtcgat taacccctgc atcaagccac cgcgccaaca aagcgcatca tgtccgccgt
     4381 caacctgacg ccagaaaatg gattctttta tagggtgtaa ttgcgcatac agcgcctgat
     4441 tggcccgcat gatcatcggc tcgcgaatac ccgcttccag cacaatgcgc agaccttcgg
     4501 cgctaacttc accagctttt agcttttcaa gtaacacgcc ctcttgctgc ccgccccgat
     4561 gcggccacca gtacgatcct gactggctta atacacagcc aaagcgttca ggccagtgca
     4621 gtccggcata cagcgcggac agcccaccaa aactctgccc ggcgaccacg gtgcgatcgg
     4681 cgcgatcgct aaaaggggca atagctttca ccaggggtaa taactcttgc tgtactgcca
     4741 gccagaaatc cgcattacac ggcagttcgt gggcgcggtg cgtggtgtcg atagcgtcga
     4801 tcaacacata cacggcggga ggaagttgct gacgatgggt cagcgaaatc agcacaggcc
     4861 agacgggcat actttgcgcc caaaattcgc catcgagcaa aactgccagc gggcgttctt
     4921 ctgctgttac atcgccggtg gtaaaaatcc atacacgacg tgaatttttc aaccgttcac
     4981 ttttccagat aatttctttg gctggtattt ctggcgcttg cggacaatcc catcccggtt
     5041 gcagaggcgc ttgcggcatt tcgagtgcag aaacagcgtg ccctcgcccg cctttccagc
     5101 tttgtggatt cagcggatcg gctattgcct ggggtaatag ttttcgccag ccttcacgta
     5161 attcgaggcg atcgggggat ggtgcagaaa aaatgtcatc gcgttcggtg ggaataaagc
     5221 agtagctgcc gcgccagttg gcattgagtt gtgtcgtcca ctgccagaca tcagtacctg
     5281 caattcgctg catcgactgg ggctggctgt tctgatgatg atcggtcaca ccagtgatgt
     5341 agatccatac gcgctttatc gtcgagtatt cttcagaacc ttggggatca cgccaccaga
     5401 aagtgacctc aaacatttcg tcattcagac gctgccattc cgggccatgt ttcgactgcc
     5461 accagctctc acttcctact tttaacgccg tcacaccata accccatgtt tactgtgcaa
     5521 tttttcattg attgcagaaa tatattgata atattattga taactatttg catttgcaat
     5581 agcgtaatgg cgcgccgtgg gaagcgcgga cattaattaa ccaactgcac tgcgtgtctt
     5641 tcaggatcaa aggttttcgc ggtagcggga tgcgtcgtgt tgatgacgac catgcccgac
     5701 agttgcaatt cgtggcaaaa atgcaggaat aaaacaatga acaagaagat tcattccctg
     5761 gccttgttgg tcaatctggg gatttatggg gtagcgcagg cacaagagcc gaccgatact
     5821 cctgtttcac atgacgatac tattgtcgtt accgccgccg agcagaactt acaggcgcct
     5881 ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc ccgttgcccg cgatgtttcg
     5941 gagatcatcc gtaccatgcc aggcgttaac ctgaccggta actccaccag tggtcagcga
     6001 gggaataacc gacagattga tattcgcggt atgggtccgg aaaacacgct gattttgatt
     6061 gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg gctggcgtgg cgagcgcgat
     6121 acccgtggtg atacctcctg ggtgccacct gaaatgattg aacgtattga agttctgcgt
     6181 ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg gcgtggttaa catcattacc
     6241 aaaaaaggca gcggcgagtg gcacggctcc tgggacgcat atttcaatgc gccagaacat
     6301 aaagaggaag gtgccaccaa acgcactaac tttagcctga ccggtccgct gggcgacgaa
     6361 ttcagcttcc gcttgtatgg caacctcgac aaaacccagg ctgacgcgtg ggatatcaac
     6421 cagggtcatc agtccgcgcg tgccggaacg tatgccacga cgttaccagc cgggcgcgaa
     6481 ggggtgatca ataaagatat taatggcgtg gtgcgctggg acttcgcacc attgcaatcg
     6541 ctggaactgg aagcgggtta cagccgccag ggtaacctgt atgcgggcga tacccagaac
     6601 accaactctg acgcttacac tcgctcgaaa tatggcgatg aaaccaaccg cctgtatcgc
     6661 cagaactact cgctgacctg gaacggtggc tgggataacg gcgtgaccac cagcaactgg
     6721 gtgcagtacg aacacacccg taactcgcgt attccggaag gtctggcggg cggtaccgaa
     6781 gggaaattta acgaaaaagc gacacaggat ttcgtcgata acgatcttga tgacgtgatg
     6841 ctgcatagcg aagttaacct gccgattgat ttcctcgtta accagacgct gacgctgggt
     6901 acggagtgga atcagcagcg gatgaaggac ttaagttcca acacccaggc actgaccgga
     6961 acgaataccg gtggcgctat tgatggcgtg agtgccaccg accgtagccc gtattcaaaa
     7021 gcagaaattt tctcgctgtt tgccgaaaac aacatggagc tgactgacag caccatcgta
     7081 acgccggggc tgcgtttcga tcatcacagt attgtcggca ataactggag cccggcgctg
     7141 aacatatcgc aaggtttagg cgatgacttc acgctgaaaa tgggcattgc ccgcgcctat
     7201 aaagcgccga gcctgtacca gactaacccg aactacattc tctacagtaa aggtcagggc
     7261 tgctatgcca gcgcgggcgg ctgctatctg caaggtaacg atgacctgaa agcagaaacc
     7321 agcatcaaca aagagattgg tctggagttc aaacgcgacg gctggctggc gggcatcacc
     7381 tggttccgta acgattatcg caataagatt gaagcaggct atgttgctgt agggcaaaac
     7441 gcagtcggca ccgatctcta tcagtgggat aacgtgccga aagcggtggt tgaaggtctg
     7501 gaaggatcgt taaacgtacc ggttagcgaa acggtgatgt ggactaataa catcacttat
     7561 atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt cgatcatccc ggagtatacg
     7621 ttgaactcaa cgctgagctg gcaggcacga gaagatttgt cgatgcaaac gaccttcacc
     7681 tggtacggta agcagcagcc gaagaagtac aactataaag gtcagccagc ggttggaccg
     7741 gaaaccaaag aaattagtcc ttacagcatt gttgggctga gcgcgacgtg ggatgtgacg
     7801 aagaatgtca gtctgaccgg cggcgtggac aatctgttcg acaaacgttt gtggcgtgcg
     7861 ggtaatgccc agaccacggg cgatttggca ggggccaact atatcgccgg tgccggggcg
     7921 tatacctata acgagccggg acgtacgtgg tatatgagcg taaacactca cttctgatgc
     7981 taacgtcaga ttgttgacaa agtgcgcgtc gttcatgccg gatgcggcgt gaacgcctta
     8041 tccggcctac aaaaatcttg ccaattcaat atattgcagg accatgtagg cctgataagc
     8101 gtagcgcatc aggcagtttt gcgtttgtca tcagtctcga atatggtcga tatgaaaact
     8161 acgcatacct ccctcccctt tgccggacat acgctgcatt ttgttgagtt cgatccggcg
     8221 aatttttgtg agcaggattt actctggctg ccgcactacg cacaactgca acacgctgga
     8281 cgtaaacgta aaacagagca tttagccgga cggatcgctg ctgtttatgc cttgcgggaa
     8341 tatggctata aatgtgtgcc cgcaatcggc gagctacgcc aaccggtgtg gcctgcggag
     8401 gtatacggca gtattagcca ctgcggggct acggcattgg ccgtggtttc ccgtcaaccg
     8461 attggcgttg atatcgaaga aattttttct gcgcaaacag cgaccgaatt gacagacaac
     8521 attattacac cagcagaaca cgagcgactc gcagactgcg gtttagcctt ttctctggcg
     8581 ctgacactgg cattttccgc caaagagagc gcatttaagg caagtgagat ccaaactgat
     8641 gcaggttttc tggactatca gataattagc tggaataaac agcaggtcat cattcatcgt
     8701 gagaatgaga tgtttgctgt gcactggcag ataaaagaaa aaatagtcat aactctgtgc
     8761 caacacgatt aattgacaac atctggtacg attcgcccgc agccatcact gactgcgggc
     8821 gaaagtgtaa agcaggtgcc ttaccactct gacctgacaa ccggatatgc ggggattgct
     8881 ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac tcaatattac
     8941 gttcctttac ggtgaactca cacagcgagt ctccgaccag aagcgtaaat cccagcaccg
     9001 tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt atattgcctc
     9061 catgcgaaga agggactacc atccgagttg ttggggctcg gagtggcagc cccaggattg
     9121 atagaaatat ctcctggggc ttcatctttc cagacctcag agttaacctg aaaccggaaa
     9181 gcttcaggca cccgccgtta tcttaccctt ccttagcaaa atagctgttt atttatacag
     9241 tgaatcctgt ttcggacttc tgtcggcaaa actcatcatt gctcttaaaa cgaaatcgcc
     9301 cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt gccttaccat cctgacctga
     9361 caaccggata tgcggggatt actccccgca caacggctac ttcttcggtt cgtaagcgag
     9421 aacagccctg aactcaatat tacgttcctt caccgtaaac tcacacagcg agtctccgac
     9481 cagaagcgta aatcccggta ccgtcaaaca cagcactatg actgccacaa gggcatattt
     9541 cgtcagcatg tttatattgc ctccatgcga agaaggaact accatccaaa ttgtctggtt
     9601 tggagtggca gccccagggt tgatagaaat atctcctgga gcttcatctt tccagacctc
     9661 agagtaaacc tgaaaccgga aagcttcagg cacccgccgt tatcttaccc ttcctcaaca
     9721 aaatagctgt ttatttatac agtaaatcct gtttcggact tctcactcct catttaatca
     9781 actgaagctg agcccgtttt ttctgcatgc ctcaatcgtc aaaattcttc gccaccagcc
     9841 agccgtgcca taacttgtca cattgcaaaa gtagccaaat tgcttcagtc accatcacca
     9901 aaaaaagaaa cattccagaa cttccgcatc tcgcccagcc gctccgactt ctatactgaa
     9961 tagaaaactc caacataaga gaaacctatg ccattacccg attttcatgt ttctgaacct
    10021 tttaccctcg gtattgaact ggaaatgcag gtggttaatc cgccgggcta tgacttaagc
    10081 caggactctt caatgctgat tgacgcggtt aaaaataaga tcacggccgg agaggtaaag
    10141 cacgatatca ccgaaagtat gctggagctg gcgacggatg tttgccgtga tatcaaccag
    10201 gctgccgggc aattttcagc gatgcagaaa gtcgtattgc aggcagccgc agaccatcat
    10261 ctggaaattt gcggcggtgg cacgcacccg tttcagaaat ggcagcgtca ggaggtatgc
    10321 gacaacgaac gctatcaacg aacgctggaa aactttggct atctcatcca gcaggcgacc
    10381 gtttttggtc agcatgtcca tgttggctgt gccagtggcg atgacgccat ttatttgctg
    10441 cacggcttgt cacggtttgt gccgcacttt atcgcccttt ccgccgcgtc gccatatatg
    10501 cagggaacgg atacgcgttt tgcctcctca cgaccgaata ttttttccgc ctttcctgat
    10561 aatggcccga tgccgtgggt cagtaactgg caacaatttg aagccctgtt tcgctgtctg
    10621 agttacacca cgatgatcga cagcattaaa gatctgcact gggatattcg ccccagtcct
    10681 cattttggca cggtggaggt tcgggtgatg gataccccgt taacccttag ccacgcggta
    10741 aatatggcgg gattaattca ggccaccgcc cactggttac tgacagaacg cccgttcaaa
    10801 cataaggaga aagattacct gctgtataaa ttcaaccgtt tccaggcctg ccgctatggg
    10861 ctggaaggcg tcattaccga tccgtacact ggcgatcgtc gaccactaac ggaagacacc
    10921 ttgcgattgc tggaaaaaat cgccccttct gcacataaaa ttggtgcatc gagcgcgatt
    10981 gaggccctgc atcgccaggt cgtcagcggt ctgaatgaag cgcagctgat gcgcgatttc
    11041 gtcgccgatg gcggctcgct gattgggctg gtgaaaaagc attgtgagat ctgggccggt
    11101 gactaagccg ggaattgcct tacagaagaa tatccctgac aatggtgttt taacttacat
    11161 ttaacaatgt tatgaaacac cctttagaaa ccttgaccac cgcagcaggc attttgctga
    11221 tggctttcct ctcttgcctg ctgctgcccg cccccgcact ggggcttacg ctggcacaaa
    11281 aactggtgac cacgttccat ctgatggatc ttagtcagct ttacacttta ttgttttgtc
    11341 tgtggttttt agtgctgggc gctattgagt attttgttct gcgctttatc tggcgacgct
    11401 ggttctcgct ggcggattaa acgtcgatta tcgccgtact tcgcggcata ctttgcttat
    11461 tctccttcgc ctaaaggaac gtttatggat aagcaatcac tgcacgaaac ggcgaaacgc
    11521 ctggcccttg agttaccctt tgtcgagctt tgctggcctt ttggcccgga gttcgatgtt
    11581 tttaaaattg gcggcaagat ttttatgctg tcgtcggagc tacgcggcgt cccctttatc
    11641 aatctgaagt ccgatccaca aaaatccctg ttaaatcagc aaatataccc aagcattaag
    11701 ccagggtatc acatgaataa aaagcactgg atttcggtgt atcccggcga ggaaatctcc
    11761 gaagcgttac ttcgcgatct gatcaacgat tcgtggaatc tggtggttga tggtctggct
    11821 aaacgcgatc aaaaaagagt gcgtccaggc taaagcggaa atctatagcg catttttctc
    11881 gcttaccatt tctcgttgaa ccttgtaatc tgctggcacg caaaattact ttcacatgga
    11941 gtctttatgg atatcatttc tgtcgcctta aagcgtcatt ccactaaggc atttgatgcc
    12001 agcaaaaaac ttaccccgga acaggccgag cagatcaaaa ctctcctgca atacagccca
    12061 tccagcacca actcccagcc gtggcatttt attgttgcca gcacggaaga aggtaaagcg
    12121 cgtgttgcca aatccgctgc cggtaattat gtgttcaacg aacgtaaaat acttgatgcc
    12181 tcgcacgtcg tggtgttctg tgcaaaaacc gcgatggacg atgcctggct gaagctggtt
    12241 gttgaccagg aagatgctga tggccgcttt gccacgccgg aagcgaaagc cgcgaacgat
    12301 aaaggtcgca agttcttcgc cgatatgcac cgtaaagatc tgcatgatga tgcagagtgg
    12361 atggcaaaac aggtttatct caacgtcggt aatttcctgc tcggcgtggc ggctctgggt
    12421 ctggacgcgg tacccatcga aggttttgac gccgccatcc tcgatgcaga atttggtctg
    12481 aaagagaaag gctacaccag tctggtggta gttccggtgg gtcatcacag cgttgaagat
    12541 tttaacgcta cgctgccgaa atctcgtctg ccgcaaaaca ttaccttaac cgaagtgtaa
    12601 ttctcccttg ccgggcatct gcccggctat ttcccctcag attctcctga tttgcataac
    12661 cctgtttcag ccgtcatcat aggctgctgt tgtataaagg agacgttatg caggatttaa
    12721 tatcccatgt tgaagattta gcgggtattg agatcgatca caccacctcg atggtgatga
    12781 ttttcggtat tatttttctg accgccgtcg tggtgcatat tattttgcat tgggtggtac
    12841 tgcggacctt cgaaaaacgt gccatcgcca gttcacggct ttggttgcaa atcattaccc
    12901 agaataaact cttccaccgt ttagctttta ccctgcaggg gattatcgtc aatattcagg
    12961 cggtattctg gctgcaaaaa ggcaccgaag cggcagatat tctgactacc tgcgcgcagt
    13021 tgtggatcat gatgtatgcg ctgctttcag tcttctcgct gctggatgtt attttgaatc
    13081 tggcgcagaa attcccggca gcatctcagt taccgctgaa agggatattt caggggatta
    13141 aactgatcgg cgcgattctg gtcggcattt tgatgatctc gctgctgatt ggtcagtcgc
    13201 cagcgattct gatcagcggt cttggtgcaa tggctgccgt gctgatgttg gtatttaaag
    13261 atccgattct tggtctggtg gcaggtattc agctttccgc gaacgatatg ctgaaactgg
    13321 gcgactggct ggagatgccg aaatacggcg cggatggcgc ggtgatcgat attgggttaa
    13381 ccaccgtcaa ggtgcgtaac tgggacaata ccattaccac tattcccacc tggtctctgg
    13441 tttctgactc ctttaaaaac tggagcggga tgtcagcatc tggcgggcga cgtattaagc
    13501 gcagtatcag tattgatgtc accagtattc gttttcttga tgaagacgaa atgcaacgtc
    13561 tgaataaagc gcatttgtta aagccttatt taaccagccg ccatcaggaa attaatgagt
    13621 ggaatcgcca gcaaggttct acggagtcgg tattaaacct gcgccgaatg accaatattg
    13681 gaacctttcg tgcctatctg aacgaatatc tgcgtaacca tccgcggatt cgtaaagata
    13741 tgaccttaat ggtacgccaa ctggctccag gtgataacgg tttaccgctc gagatctatg
    13801 cgtttaccaa caccgtggtg tggctggaat atgaaagcat tcaggctgat atattcgatc
    13861 acatatttgc gattgtcgaa gagtttggtc tgcgacttca tcagtcgcca accggcaatg
    13921 atattcgctc tctggcgggt gcatttaagc agtaattaaa aaaaccgctc tcatcgaatg
    13981 gatgagagcg gacatcgttt tgtcggatgt agcgtgaatg ccttatttcc gacgcagcgt
    14041 tttaaatgct acaaacagga atacaatcca caccggcagc aggatcgctg acaagcgcat
    14101 atcatccatc gtgcacatca gcagcaaaat catggcgagg aaggcgatgc aaagatagtt
    14161 gccgaacgga tagagcagcg ccttaaactg tgtttcacgc ccctgacgtc gcatcgctgc
    14221 acgaaaacgc agatgcgcca gacagatcat aatccagttc aacagcagcg ttgctaccac
    14281 cagcgccatc agcagaccaa acgctttttg cggcagcaga tagttgatta acaccaccag
    14341 cgaagtgatc gctccggaaa gcatcagcga gttaatcggc acgccgcgac ggctaacacg
    14401 ggtaaggaac ttcggcgcat taccctgcac agaaaggcca aacagcatgc ggctgttaga
    14461 gtaaaccccg ctgttataca ctgacagcga tgctaccaga atgacgaagt tcagcgcaga
    14521 agctaccacg ttgctgtcga gattatggaa aatcatcaca aacgggctac tgttggattt
    14581 cacttccacc cacggataga gcgccagtaa aaccaccagt gaaccgatgt aaaacagcag
    14641 gatgcgatac accacctgat ttaccgcttt tggaatgctt ttttccggat cgcgcgcttc
    14701 agcggcagta atcccaatca gctccagacc gccgaaggag aacataatta ccgccagcga
    14761 caaaatcagc ccattccagc cggtggcgaa gaaaccaccg tagcgccaga ggttgtcgat
    14821 actggctttc tcgccgccgt gaccagaaaa cagcagccac aggccaaagc cgatcatacc
    14881 gatgattgcc agcactttaa tcagcgcaaa ccagaactcg gtttcgccat ataagcgcac
    14941 gttcaccagg ttaacggcgt tgatgataat aaagaaggcg gcagcccaaa tccacgttgg
    15001 aacatccggg aaccagtact gcatatagat gcccgcagcg gtcagctctg ccattcccac
    15061 cagcacgaac atcacccagt agttccagcc ggagaggaac ccggcgaacg gtccccagta
    15121 tttataggca aagtgggcaa atgaaccgga taccggctct tcgaccacca tctcgccgag
    15181 ctggcgcata atcaggaaag cgatgatccc ggcgacgccg tagcccagca atacagccgg
    15241 acccgccatc tgaatcgccg ggccaatgcc aagaaacaga ccagtaccaa ttgcgccacc
    15301 cagcgcaatc agttgaatat gacggttatg taatccgcga tgaagcgtcg gctcttgatt
    15361 cgacgcagta tcttccgata cgtttgacgc gtttttcacg cctttcccct gtgtgtcttt
    15421 tttgttgagg ggcacctttt aacatttagt gcccatcgtc gcaagacaca atccacacgg
    15481 ttaaaccggg tatcctgctt ttatttccgt acccgatgtc ggtgcagcca catcagctta
    15541 tacgctgccg ggataataaa cagcgacagc aaaggcgcgg tgatcatgcc gccaatcatc
    15601 ggcgcggcaa tccggctcat cacctctgat cctgcccccg ttccccacag aatcggcagc
    15661 agaccagcga taatcaccgc caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg
    15721 tgatgtaacg cctcatccag cttctgctcg ctgaatgttt gcggattatt caacgacggc
    15781 tcggcctcta tggcgtgacg taaatacatc agcatcacca cgccaaattc ggcggcgacc
    15841 ccggcaaggg caataaaacc ggtgcccgtc gccacagaaa gatgaaagcc catccaccac
    15901 agcaaccaga tgccgcccac cagcgcaaac ggtacgctgc tgataatcag caacgcttcg
    15961 cccacccgac ggaacgccag atacaacagc acgaagatga tcatcaacgt catcggcacc
    16021 atgagtttca gcttatggtt ggcgcgctcc agtagctcga actgcccgga gaatgccacg
    16081 ctggtgcccg gttttaactg cactttttca gctatcgctt tttgcaaatc gtgaaccacc
    16141 gacaccatgt cacgatcgcg ggcatcgata taaatccagc tcgtcgggcg cgcattctcg
    16201 gttttcagca tcgacggtcc ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt
    16261 tgctgcttca tcggcgtcag gatcggcaac tggcgcagtg cctgcggact atcgcgccag
    16321 ctttgcggat aacgcagatt aattggataa cgggcaatcc cttccaccgt ttcgccaacc
    16381 atcgccccgc ccaccgcaga agtcacaaac aactgcacat ccgccaccgt cataccgtaa
    16441 cgtgcggctt tttcacggtt aatctcaacg ttgatatagc gcccaccttc cagccgctcg
    16501 gcaagcgcag aagccacgcc tggcaccgtt cgtgccacct cttcaatttg ctccgccatc
    16561 gcgtcgatat ccgccagcac agtgccggaa acttttatgc cgatagggct tttaatgccg
    16621 gttgagagca tatcgatacg gttacgaatt ggcggcaccc acagattcgc cagccccggc
    16681 agacgcacgg tgttatccag ttcctcaatg attttgtcca ttgtcatgcc tggccgccac
    16741 tgctcctgcg gcttaagctg gatggtcgtt tctaccatct ccagcggagc agaatcggtg
    16801 gcggtttccg ctttcccggt tttgccaaat acccgcgcca cttcaggtac gctcataatt
    16861 agcttgtcgg ttttttgcag catactcgcc gcctctgctg cggaaatccc cggcagcgtc
    16921 gatggcatat acaacaagtc gccttcattg atctgcggta aaaattcccc gccaacttta
    16981 ttgagcggcc agagaaccgt cagcaccgaa agcgctgcca ccaacagcgt ggttttcggc
    17041 cagtgcagta ctttcagcaa cagcggatga taaacacgaa tcaaaaagcg attgagcggg
    17101 ttactgcttt ccggcggaat tttgccacgg atccagtaac ccatcaggat agggatcact
    17161 acgatcgcca gcagcgccgc acccgccatc gcatacgttt tggtgaacgc cagcgggcca
    17221 aacagacgcc cttcctgccc ttccagggtg aagatcggga taaacgacag cgtgataatc
    17281 agcagactga taaacagcgc cggccccact tcaacagatg cattggtaat cacctgccag
    17341 cgcgttttat tatccagcgt ggcgtcagga tgctggtgct gccactcttc cagccgttta
    17401 tgtgcattct cgatcatgac gatagcggca tcgaccatcg ccccgacggc aatcgcaatg
    17461 ccacccaacg acataatatt ggcattcagc ccctggaagt gcatgacaat aaaagcaata
    17521 cacaacccca gcggcaacga aataatcgcc accagcgccg agcgcacatg ccagagaaac
    17581 agcgcacaga ctaccgccac cacaataaac tcttccagca acttgccgct gaggttgtcg
    17641 atggcccggt caatgagctg gctgcgatcg tatgttgtaa ctatctccac gccttccggc
    17701 agactgcttt tcagcgtttc cagtttgtcc ttcacggcgg cgatcacttc acgggcgttt
    17761 ttgccggagc gtaggatcac caccccgccc acaacttcgc cgttcagttc ggcaatgccc
    17821 cggcgcatct ccgggccaac ctggatcttc gcaacatcac gcagataaac aggcacgcca
    17881 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
    17941 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
    18001 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
    18061 ggatcgataa ccacctgata ctctttcacc acgccgccca ccgacgccac ttccgcaacg
    18121 tcagggatgg tttttagctc atatttgaga aaccagtcct gcaatgagcg taaatcggcc
    18181 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
    18241 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
    18301 tactccagca cccgcgaacg cgcccagtac ggatcggtac catcttcgaa aatgacatac
    18361 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
    18421 atggtggtag ttagcggata agtcacctga ttttcaacga tttgcggtgc ctgaccggga
    18481 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgtg
    18541 ttaatgatgg tccaggtgcc ccagatactc agaaataacg cgcccatcag caccaaaaaa
    18601 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
    18661 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
    18721 tcaggaacag gccgctggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
    18781 tgacaccttg cgatgcctgg aaaacggcaa cgcgttttgg tacaaagcgc ccgtcggcat
    18841 caacggtaat cacccgctgt tcgctgccgg tatcaatcag cgcctgtgac ggaatgagca
    18901 gcatcggttc gctggcggta ttgagttgca accaggcgtt cattcccggt tttagcgcct
    18961 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
    19021 gtagtagcgt ccatttgcgg atggtgagtg ttttatcagg tcgcgccgga acggtgagcg
    19081 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
    19141 acaccgggtc cataccctga attttcgcta ccacgttatc tttggcgata ttcattcccg
    19201 cgcgcagatc aaacgcggtg atcacgccat caatgggtgc tttgagcgta aagcgagtct
    19261 ggattttttg cgtggcgatc aggcggcgaa tatccgcctc cggcattccc gccagtcgca
    19321 gccgctcaag aatgccttcg gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat
    19381 actcactctg cgcttccacc cagtcgggaa tggtcaggtc gagaagcggc gcgccctttt
    19441 gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggcg gcgcgagcct
    19501 gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag
    19561 tcagcggccc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt
    19621 caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc agatccatat
    19681 ccataaacgg cgatttacct ggtttatcga accgcgtatt gggatacatc gggtcgtacc
    19741 agaataagat tttacgttct gcggtcgacg ttttttctgc aggcggttcc gcctttgcaa
    19801 accaggtaaa acctgccgca gaaataatac cgcccgcgat catgctgccg ataataagcg
    19861 cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag
    19921 aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta attccactca
    19981 ttttcgtctg cggggtgatg gtaaagcgca tggtcatctc cggccagttc acggcagcaa
    20041 tcggatcgtg atggatggtg atttttttgc tttccagatc aaaacctttt accacgccgg
    20101 tggcactaat gacctgtggt tgtgcttcgc tcatggtttc atgatgatgt tcgttagcct
    20161 gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca
    20221 tttattttct cctggagtta aaaattattg ctgccaaccg ccaccaagtg cggtatataa
    20281 agaaatttcg ttgacctgac gggcataatt caggtcaagt acggtttgtc gggttgcaaa
    20341 taaagaacgc tcggcatcca gcacttccag ataacttact gcgccgtgct gatataatgc
    20401 ccgcgcccgt tgcaaagtaa tttgcagcga tgccagataa cgctgctggg cgctaatttg
    20461 atcgttcagg ctttgacgta atgccagcgc atctgccact tctttaaagg cgttctggat
    20521 tttctgttca taattcacta ccgactgctg ctggcgaatt tcggcgatat ccagattggc
    20581 ctggttgcgt ccggcattaa aaatggggat ctcaatttta ggaataaaat tccacatccc
    20641 gctgctggcg ttaaataatg acgacagatc gctgctggcg gttgatattc cgctggtcag
    20701 gctgatagac gggaaaaatg ccgcgcgtgc agcaccaata ttggcattag ccgccattaa
    20761 cgcatgttca gcttccataa tatccgggcg ctgcaataag atttgcgacg acaagcccgc
    20821 cggtaattta acgctttgca ggctgtcgct gtttactgtc tgcgcttgcg gcagcttgcc
    20881 gtagcttccc agtaacagtt gcaatgcatt attcgcctgc gccagttccc cctgacgttt
    20941 agcgatgtcg ctgcgggtac tttcaatcac cccgcgagcc tgttccagcg ccagaacatt
    21001 actgctacct gtcaacagtt gtttttcgac aaacgcatat gactgctgat aattacgcag
    21061 cgtttcttcg gctatttgca attgcgcata cgccagctgc tgattgaaat agctttgcgc
    21121 gacattagaa accagcagaa tatgcaccgc gcgctgagct tcctcagtgg ctaaataatt
    21181 ttgtcgctcg gcttcgctca tgttctttaa gcgcccgaaa aaatcgaggt caaagctggc
    21241 gttaaggcca gtagaaaact cccgcgtcgt ggctgaatcg cctttaagat tgccgctcca
    21301 gctgccgctg ccctcaccat tgagctgtgg gtagcggtcg gcatcagtca gacgatattg
    21361 cgcccgtgcc tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat tctccagcgc
    21421 ctcgctaatc agcgtcttca cctgattatc aacaaaaaag gtgcgccagc ccgcgttctg
    21481 atagttatct gctgcgttaa ccaggccgtt ctggctgagt gagaactgct gcggtacggg
    21541 cattgccggg cgctgataat ccggtgccag tgaacaaccg gttagcgcaa gggccacaca
    21601 aaatggcaga agtttacaag gagacatagg ctcataattt ctggtgattt tataccgcca
    21661 actttactcg ccaggctctg attttccggt gacaggaaaa tgacaaaatt gtcattttgc
    21721 caataagcga ttgccatctg atcccgctac tctagaattg cccgggcaac atgcggagga
    21781 aatatgaaac tgttgattgt cgaagatgaa aagaaaaccg gagaatactt gaccaaaggg
    21841 ttaaccgaag ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg ctaccatctg
    21901 gcgatgaccg gagattatga cctgataatc ctcgatatta tgctgccgga cgtgaacggc
    21961 tgggatatcg tgcgcatgtt gcgctccgcc aataaaggga tgccgattct gttgcttacc
    22021 gcgcttggca ccattgaaca tcgcgtcaag gggctggagt tgggggccga tgactatctg
    22081 gtgaagccgt tcgcgtttgc tgaactgctg gcgcgggtgc gcactctcct gcggcgcggg
    22141 gcggcggtga ttatcgaaag tcagtttcag gttgccgatt tgatggtcga tctcgtcagc
    22201 cgcaaagtca cccgcagcgg cacgcgcatc actctgacca gtaaagagtt tactctgctg
    22261 gagttttttc tccgccatca gggcgaagtg ctgccccgct cgcttatcgc ctcgcaggta
    22321 tgggacatga attttgacag cgacactaac gccattgatg tggcggtgaa gcggctacgc
    22381 ggcaaaatcg acaacgactt tgagccgaag ctgattcaaa ccgtgcgcgg cgtgggttac
    22441 atgcttgagg tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc tggcaacccg
    22501 cctgaccttt tttatcagcc tggccaccat cgcggcgttt ttcgcctttg catggatcat
    22561 gatccactca gtaaaagtgc attttgccga gcaggatatt aatgatttaa aagagattag
    22621 tgccaccctt gaacgcgtac taaatcaccc tgacgaaacg caagcccgac gcttaatgac
    22681 gctggaagat atcgtcagtg gttattccaa tgtgttgatt tccctggcag atagccacgg
    22741 taaaacggtg tatcactccc ccggtgcgcc tgatatccgc gagtttgcgc gcgatgccat
    22801 acccgataaa gacgcgcggg gcggcgaggt gtttctcctc tccggcccga cgatgatgat
    22861 gccaggccac ggtcacgggc atatggaaca cagcaactgg cggatgatca gtttgccagt
    22921 tggcccactg gttgacggca aaccgattta tacgctctac atcgcgcttt cgatcgattt
    22981 tcatcttcat tacataaatg atttgatgaa taaacttatt atgaccgcat cggtcatcag
    23041 catcctgatc gtctttatcg tgctattggc ggttcataaa ggtcacgcgc cgatccgcag
    23101 cgtcagccgt caaatccaga atatcacctc gaaagatctc gacgtccgcc ttgacccgca
    23161 gaccgtgccg attgagctgg aacagctggt actgtcgttc aaccatatga tcgagcgtat
    23221 tgaggatgtc tttacccgcc agtccaattt ctcagcggat atcgcccacg aaattcgcac
    23281 accaattact aatctcataa cgcaaacgga aatcgccctc agccagtcgc gcagccagaa
    23341 ggagctggaa gatgtgctct actctaatct cgaagagctg acgcgaatgg cgaaaatggt
    23401 cagcgatatg ctgtttctcg cccaggccga taacaaccag ttaatccccg aaaagaaaat
    23461 gctcaacctg gcggatgaag tcggcaaagt gttcgatttt ttcgaggctt tagcggaaga
    23521 tcgcggcgtg gagctgcagt ttgttggcga cgagtgccag gttgcgggcg atccgctgat
    23581 gctgcgtcgg gcgttaagca acctgctttc gaacgccctg cgttatacgc cacccggaga
    23641 ggctattgta gtgcgctgcc agacggtcga tcatctggtg caagttatcg tcgaaaaccc
    23701 cggtacgccc attgcgcccg agcacttacc gcgattgttt gaccgtttct accgcgttga
    23761 cccctcccgc caacgaaaag gcgaaggtag cggcattggg ctggcgatag tgaaatcaat
    23821 tgttgtcgcg cataaaggca cggttgcggt tacgtcaaat gcgcggggga caaggtttgt
    23881 gattgtgttg ccggagaggg ggtgattatt aagttcaaat tcagggcgag ggtttacggc
    23941 gtttttcgct aaatttccat cattata
//
LOCUS       DAZFTN010000071        23813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFTN010000071 DAZFTN010000000
VERSION     DAZFTN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            196..1140
                     /locus_tag="ACHKV2_003141"
     CDS             196..1140
                     /locus_tag="ACHKV2_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940982.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
     gene            1222..1644
                     /locus_tag="ACHKV2_003142"
     CDS             1222..1644
                     /locus_tag="ACHKV2_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6940983.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
     gene            2192..2641
                     /gene="glmS"
                     /locus_tag="ACHKV2_003143"
     CDS             2192..2641
                     /gene="glmS"
                     /locus_tag="ACHKV2_003143"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO6940984.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            2641..4029
                     /gene="glmL"
                     /locus_tag="ACHKV2_003144"
     CDS             2641..4029
                     /gene="glmL"
                     /locus_tag="ACHKV2_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO6940985.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4048..5493
                     /locus_tag="ACHKV2_003145"
     CDS             4048..5493
                     /locus_tag="ACHKV2_003145"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO6940986.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            5493..6734
                     /locus_tag="ACHKV2_003146"
     CDS             5493..6734
                     /locus_tag="ACHKV2_003146"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO6940987.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            6738..8108
                     /locus_tag="ACHKV2_003147"
     CDS             6738..8108
                     /locus_tag="ACHKV2_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO6940988.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            8105..8422
                     /locus_tag="ACHKV2_003148"
     CDS             8105..8422
                     /locus_tag="ACHKV2_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO6940989.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            8583..9863
                     /locus_tag="ACHKV2_003149"
     CDS             8583..9863
                     /locus_tag="ACHKV2_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO6940990.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            9971..11623
                     /locus_tag="ACHKV2_003150"
     CDS             9971..11623
                     /locus_tag="ACHKV2_003150"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6940991.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            11633..12235
                     /gene="cobO"
                     /locus_tag="ACHKV2_003151"
     CDS             11633..12235
                     /gene="cobO"
                     /locus_tag="ACHKV2_003151"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6940992.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(12269..13174)
                     /locus_tag="ACHKV2_003152"
     CDS             complement(12269..13174)
                     /locus_tag="ACHKV2_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6940993.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(13841..14710)
                     /gene="sucD"
                     /locus_tag="ACHKV2_003153"
     CDS             complement(13841..14710)
                     /gene="sucD"
                     /locus_tag="ACHKV2_003153"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6940994.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(14710..15876)
                     /gene="sucC"
                     /locus_tag="ACHKV2_003154"
     CDS             complement(14710..15876)
                     /gene="sucC"
                     /locus_tag="ACHKV2_003154"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6940995.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(15970..17187)
                     /gene="odhB"
                     /locus_tag="ACHKV2_003155"
     CDS             complement(15970..17187)
                     /gene="odhB"
                     /locus_tag="ACHKV2_003155"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6940996.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(17202..20003)
                     /gene="sucA"
                     /locus_tag="ACHKV2_003156"
     CDS             complement(17202..20003)
                     /gene="sucA"
                     /locus_tag="ACHKV2_003156"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6940997.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(20304..21020)
                     /gene="sdhB"
                     /locus_tag="ACHKV2_003157"
     CDS             complement(20304..21020)
                     /gene="sdhB"
                     /locus_tag="ACHKV2_003157"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO6940998.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(21036..22802)
                     /gene="sdhA"
                     /locus_tag="ACHKV2_003158"
     CDS             complement(21036..22802)
                     /gene="sdhA"
                     /locus_tag="ACHKV2_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO6940999.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(22802..23149)
                     /gene="sdhD"
                     /locus_tag="ACHKV2_003159"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(22802..23149)
                     /gene="sdhD"
                     /locus_tag="ACHKV2_003159"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO6941000.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(23143..23547)
                     /gene="sdhC"
                     /locus_tag="ACHKV2_003160"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(23143..23547)
                     /gene="sdhC"
                     /locus_tag="ACHKV2_003160"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6941001.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
BASE COUNT         5991 a         6160 c         5875 g         5787 t
ORIGIN      
        1 gttatgttat tgtttcatta gtaataatca cttaaatgta tcgatgaaac aggtatgact
       61 taccgggagt gattgtttct gagtgaacag aggccacttt cgcctgataa atgaaaaatg
      121 agcgattttt gcaagttagt ttttctatag ccgagtacgc tcagcgcctc gcttgattac
      181 aatgaggttg ctgctatgag tcctgataat atcggttcgg ctgtcaatcg cttctcaggt
      241 tggccatcta atgattctct aaaaacagat aaagaatcaa tgacatcacc cgtgactgtg
      301 caggtaataa agatgattgc cgccatcctt gacccacatc ggatagtcgt tggaattgat
      361 acactttata caacgcagcg cgaaacattt acctcagaga gtcaacagct cattgaatca
      421 gcattgcaaa cacttcatgt gatgaattta aataatctgg aaacaaatcg cgatgaagaa
      481 agtcactatc tggcttttga atataacgtt gacacctctg acagaataac cgtttctgct
      541 tataatgaaa aaaaacctgg ctgctatggc caatgtcttg taatacagga tgacgaaaca
      601 gacttattta acctgataaa aattaaaatt ttcgaagcca ggcataatta tgtcgctcaa
      661 ttgatgagcg atccggaatt tatgttcaaa ttcagttata gcgctcaaca aaatagatta
      721 gaacctcatc ttctggttcc cgcttatttc cctctaaaaa caaacgaacc ggttacgcaa
      781 gaggatgtac tgttacttta tcgtttcttt aaaatgaatg atgattttaa taaattaaca
      841 tcagatgaat acatgagtat cctgacacct ctgatgaagt gtgagcgtag cgtccacgac
      901 aacaacaggt atgtgacggg caaagatacc cttcttctgg actatcctcc ttctggtaac
      961 cagatacatt tccatgtttt ccctgacgaa tcagcaacac tagtccttta tttttcaaac
     1021 aatgacattg aatgtttcgt ctttgaaaga gatattccga gccagtatcg ctttttcaaa
     1081 atgtttacca atctggcact ggttatagat cagttaaccg aagccaataa aatactttaa
     1141 ttttctatat atcccctatg gcaaatattg ctaaacaatg cctggggatt aatttttatt
     1201 actcacggaa agatttaaat aatgatacca tctcagatct ctttcaatac ccttccagtc
     1261 aatgcaacat acgcttcaga gacaacagtt gacacacaaa aattctctga catcctctac
     1321 tcagcggggt gttccctaaa ggatatggct tatctgagca gatgtctggc atcaatacac
     1381 ccgactctcg ccaaaaacat ttatgaaaca gaaaatctca gcgatcaaaa attactacat
     1441 attgattgcc gtagtacaaa tgaaatcaag ataaatatct tttttggtca acaacgagaa
     1501 ggattaattg aaattaattc tgacacagtc attttttcga tattgtcttc attaattgac
     1561 tccgccattt ctaaatttca acaccaaatc cccgttaacg gttcagttaa tcgtgaacta
     1621 ttgtatgagg attatactga ttaaatccac cctgtcgcga aaaatggcct gcgttattgc
     1681 cagacgtaat aaaacctttg attaacgtta ctcatactga tagcaccttc atgtacctct
     1741 cttttttacg aaaaattaaa agagctaact aaggtcataa ttcgcatgaa tcattaacca
     1801 tttgttattt tcaacattat ttttacatta agcagagatg aattgcagtg aaattcgact
     1861 taaatcttat gaaatgcatt tactcatggc taaaaccacg tcacaaaaat gcaaattaga
     1921 agtagatcac aattatctca atacattaaa tcaagttaat tttaaaaatt taacactcat
     1981 gacattagtt gacataacac actgaattaa catggaaatt taaaaacaca cttcaaacaa
     2041 tcatttcccg aaagacattt tgaaaataga tcaattgcaa cattacggtc agataaagac
     2101 actcctgtga tattaaacct gtgccgttat gaacggccat aaatacgacg tgtgtaactt
     2161 attttactct ctgtcttaca aaggattacc catgaagaaa gcaacacttg tgattggcgt
     2221 tattggcgcg gactgccatg cagtaggcaa taaagttctg gaccgcgttt ttagcaacca
     2281 cgacttcaga gtcattaatc tgggcgtaat ggtcagccag gacgaatata ttgatgctgc
     2341 cattgaaact ggcgctgacg cgattgtcgt ctcctctatc tatgggcatg gcgatatcga
     2401 ctgtctgggc atgcgcgaac gctgcatcga gcgtggactg ggggacatcc tgctctatgt
     2461 gggcggtaac ctggtggtcg gaaaacatga cttcgctgac gtggaaacca aattcaaaga
     2521 gatgggattt gatcgcgtct ttgccccaag tcatgactta gaagatgttt gccagctgat
     2581 ggcccacgat atcaaccaac gtcatgacgt tgatactcgt attcttgaag aggctatctg
     2641 atgcaaatcg tctctgtcga tattggatcg acgtggacca aagcagccct ctttacccgg
     2701 gagggggacg cgttaacgtt ggttaaccac gtactgaccc caaccaccac ccatcatctg
     2761 gcgaaggggt ttttctccag cctgaaccag gtgttaaacg tagataatgc cctccccctg
     2821 ttaaacagcg gtgaggttgc attgaagtat tcctcctctg ccaaaggtgg gctggcggtt
     2881 gccgcaatgg gattggtgcc ttctatcacc ctggaaaccg cgaaagtaac cgctcactct
     2941 gcgggtgcta aaattgcgca atattacgcc tacaagctga accgccgtga tatccaggca
     3001 ctggaagaaa cgcagcccga tattctcttg ttcaccggcg gcaccgacgg cggagaagaa
     3061 agctacggac tgaataacgc ccgtgtgctg gcagaatcca aactcgactg cgccattatt
     3121 tatgccggaa accgggatat tcaggacgag gtgcaggaga tcctcgggca taaagacctg
     3181 accattgtcg acaacgttct gcccgatctt gaccatccga accctgtagc tgcacgccag
     3241 gctatttgcg acatattcct taaacgcatc gttaaaggga aagggctgga tgtcattgtt
     3301 gataaaacag gcgaagaacc aatgccgaca ccatggacgg tctttgaact ggtcaaggca
     3361 atcagtaacg tcgatcacag ctggaaagag ttcatgctga tcgacatggg cggcgcaacc
     3421 accgatgttt attccgcctg tgccaacacg ctctcgcccg acaccgtttt acacggcgta
     3481 ccggaaccct tcgtaaaacg gacggtggaa ggcgatctgg gaatgcgcgt ctcggcagtg
     3541 gtggttggtg aaagtgcgaa agagttagtc aatgtgaatt ttgctcagca acccgcacag
     3601 ctggacgctt tttatcatta cctccgtcat ctagtcgctc atcccgacta cttacccgca
     3661 aatgcagaag agaaatattt cgataccgtc ctggccggac tgtgtgtggg ttatgccacc
     3721 gaaagacatg cgggcaccaa aaaagaggtc tgcacctgcg tcggcaatgt cgatttacag
     3781 atgggacgtg acctgacaac cgtgcgtaaa gtcgtcggat ccggcggatg gctctcgcgt
     3841 gccagtcagt tcgatatgca tcactggttg aaataccgcg agttaaacga cgacggtaaa
     3901 cgcattctct taccgaccga atttgaatat taccgcgatt cccgggggct attaccactg
     3961 ctggcaaacg tcgccagagt cgatccgctg gccgccgcgc gcaccagtat tcaatgctta
     4021 accctataac aattaaggct gcaatcaatg gaacttcgaa ataagaaatt aacccatgac
     4081 gaattcatga ccgagcggca acaggtactg aaaacctggg aaaccggcaa ggatgttgaa
     4141 aactttgaag atggcgttaa ataccaacaa accatccctg agcataaacg tttctctctt
     4201 gcgctgctga aagcagacaa ggaaggtaaa acccttagcc aaccgcgcgc aggtgtggcg
     4261 ctgatggacg aacacatcga actgctgaaa acactacaag aagagtgcga tctgctgcca
     4321 agcaccatcg atgcatatac ccgtctgaat cggtacgaag aagcggctgt agggattaaa
     4381 aaatccatcg aagccggaac ctctaaactg aacggtttac cggtagttaa ccatggtgtc
     4441 gcagcttgcc gtcgcctcac cgaaaccctg caaaaaccgc tacagatccg ccacggcacg
     4501 ccagatgcgc gtctgctggc tgaaatctca atggccagcg gctttaccag ttatgaaggc
     4561 ggtggcattt cctacaacat tccgtatgcc aaacgcgtaa cgctggaaaa atcgatccgc
     4621 gactggcagt actgcgaccg tttaatgggg atgtatgaag aacacgggat ccgcatcaac
     4681 cgcgaaccgt ttggcccact aacgggtacg ctgatcccgc cgtttatctc gcactctatc
     4741 gcgataatcg aaggcttgct ggcgctggaa cagggtgtga aatctatcac cgttggttat
     4801 ggccaggtcg gcagcctgac gcaggatgtt gcggcgatcc agtctctgcg tgaacttgca
     4861 cacgaatatt ttcagagcta tggctatacc gactatgaat tgagtaccgt gtttcaccag
     4921 tggatgggcg gctttccgga agacgaatcg aaagcgttcg ctattatctc ctggggcgca
     4981 gccgtcgcgg ggatgtctgg tgccaccaaa gtcatcacca aaagtccgca cgaagcctgg
     5041 ggtatcccga ctgccgctgc caacattcag gggctgaaag cctcccgcca aatgctcaac
     5101 atggtcaatg agcagaaatt cccgccgtgc cctgcggttg aactggaaat tgaactgatt
     5161 aagagcgagg ttcgcgcggt actgaataaa gtgttcgaac tgggtaatgg tgatattgcg
     5221 cgcggaacgg tgctggcatt tgaagcgggc gtactggatg tgccgtttgc gccagccgcc
     5281 tgcaacgcgg gcaaaattct tccggtccgt gacaacaccg gcgcgatacg cgtactggaa
     5341 gccggagccg ttccattgcc aaaagatatt ctcgacctcc accacgatta cgttgcggaa
     5401 cgcgcacgct gtgaaggacg ccaaccgaca ttccagatgg ttgtagatga catcaatgct
     5461 gtatcccaca gtaaattaat aggaagacca taatgaaaat aaaacaggct ctgttcaccg
     5521 ctggctactc ctcattctat ttcgatgacc agcaggcgat aaaaaacgga gcgggtcatg
     5581 acggcttttt ttataccggg gagccagtaa cacaggggtt taacgccgta cgtcaggccg
     5641 gggagtgcgt atcggtacag ttgattctgg aaaacggcgc ggtcgccgta ggtgactgta
     5701 ctgccgtaca gtattccggg gcaggcggtc gcgatccact gttcctcgca gagcacttta
     5761 ttccgttcct caacgaccat atcaagccat tactggtagg ccgcgatgtg gatgctttcc
     5821 tgccgaatgc ccgtttcttc gacaaattgc gtattgacgg caacttgctg cataccgccg
     5881 tgcgctacgg attatcacag gcgctgcttg atgctaccgc gctggcaacc ggccgtctga
     5941 aaactgaagt ggtctgtgat gaatggcagt tgccacgcgt ggcggaatcc attccattat
     6001 ttggtcagag cggcgacgat cgatatatcg ccgtcgataa gatgatcctt aaaggcatcg
     6061 acgtgctgcc ccatgcgctg attaataacg tcgaagagaa gctgggcttt aaaggtgaaa
     6121 aactgcgcga atatgtccgc tggttgtcgg atcgcattct aagcaagcgc accagcgcac
     6181 gctaccaccc taccctgcac atcgatgtat acggcactat cggtctgatc ttcgatatgg
     6241 atccgcttcg ctgtgcgcaa tacatcgcca gcctggaaaa agaagcgcaa ggcctgccgc
     6301 tctacatcga agggccggtc gatgccggta acaagcccga tcaaattcgc ctgctgaccg
     6361 cgattaccaa agagctgacg cgcctcggtt ccggcgtgaa aattgtggcc gatgaatggt
     6421 gtaacaccta ccaggatatt gttgatttca ctgatgctgc cagttgtcac atggtgcaaa
     6481 tcaaaacccc ggatctgggc agtattcaca acatcgtcga tgcggttctt tactgcaaca
     6541 gccacagcat ggaagcgtac cagggcggca cctgcaatga aactgatgtc agtgcccgca
     6601 cctgtgtcca cgtcgccctt gccgctcgcc ccatgcgtat gctggtaaaa ccagggatgg
     6661 gctttgacga aggcctcgat atcgtcttca acgaaatgaa tcgtactatc gcgctgttgc
     6721 aggctaagga ttaacaaatg gctcgttctt ttaaagtctt atcgccaacg gcaatcctcg
     6781 ggtatggatt cccggaagag agcttccgca aagcgatggc agaatcgccc gatctgatcg
     6841 ccgttgatgc aggctcttcc gatccaggcc cccactacct cggggcaggc aaaccgttta
     6901 ccgatcgcgc aggggtcaag cgcgatctgc gctatatgat caccgcaggc gtgcaaaata
     6961 atataccggt ggtgatcgga acggctggcg gttctggtgc cgcaccacac ctggagtggt
     7021 gccgagaaat aattcatgag attgcccggg aagagcacct ctcgttctcg atggcgttga
     7081 tcccggcaga cgttgacaaa gccatcgtcc accaggcgct ggataacggc aaaatcacgg
     7141 cgctggattt tgtcccgccg ttaacccacg acgcgattga cgaaagtacg tatatcgtcg
     7201 cgcaaatggg catcgaaccc ttccagcggg cgctgaaaga acgcgcgcaa gtggtgctgg
     7261 gcgggcgcgc ttacgacccg gcctgctttg ctgcgctccc gatcatgcaa ggctttgatg
     7321 aaggtctggc gctgcattgt gggaaaatcc ttgaatgcgc ggcaatcgcc gcaacgcccg
     7381 gctcaggctc tgactgtgcg atgggcatca ttgatgacaa cggctttacg ctgaaaacat
     7441 ttaatccgaa gcgtaaattt accgaaacgt cagcagctgc acacacactg tatgagaaat
     7501 ccgatcccta cttcctgcca ggacctggcg gcgtgttgaa cctgaaaggc tgcacattta
     7561 aagcagtaaa tgacggcgaa gtctacgtca gcggttctaa gcatgaagaa acgccgtatg
     7621 ccctgaaact ggaaggtgca cgacgggtgg gcttccgctg tctgaccatc gccggaacgc
     7681 gcgacccgat tatgatcgcc gggatcgata aaatcatcga tgaagtcaaa accagcgttt
     7741 cgcgtaacct gtcgctcgac gatgacagca ttcgcatcaa tttccacctg tacggcaaaa
     7801 acggggtgat gggagaccat gaaccgatgc aaactgccgg gcatgagctg gggattgtcc
     7861 ttgatgtagt tgcaccgacc caggaaattg ccaacagcgt ttgctcgctg gtgcgctcta
     7921 ccatgctgca ctacggctat gaaaaccgca tcgctaccgc aggtaatctg gcgttcccgt
     7981 tctccccttc tgatatccag ggcgggccgg tatacgaatt ttccatatat cacctgattg
     8041 aagccaacga cgctctgcgt tttgatttcc atattgaaca ggtgacgcca gaaggagttc
     8101 aggcatgaaa cagtcaattg tctcactggc gcaagtcatt cgttcgaaaa atgccggacc
     8161 ttacgaactg gttttagata ttttatttaa aacgaaagaa aactatgagc gagttaaatc
     8221 atcagggcaa ttaaccccag aactgattgc acgcttatat catgttgaac ctgactttat
     8281 tcatcgtatt gtctggtttg atccttctaa cgcagtaaaa atcgtcatgc cacgagatat
     8341 tatctccggg aatgtaggtg acaatgatgt ctatggtgca cagcaacatg cacctttatt
     8401 aaatatgagt ttcgatttgt aaaaatccga atacttttag ccaactgaca tcccgtcact
     8461 tattgcagtt caacacttca gctatttgta aggttgtaat agcaggtaga tgcctgaatc
     8521 tctattcagg catctacttc tcctgattct gatttatcgc cgtgcacctg atggaggaaa
     8581 aaatgaagaa aataagttta accacgatga ttcttttggc gctggtactt ggaatgatta
     8641 tcggcgtagt gctcaataac actgcttcac cggaaaccgc aaaactctat gcgcaagaaa
     8701 tatcgatatt cacgacgatt ttcttacgac tgataaaaat gattatcgct ccgttagtgg
     8761 tctctaccct ggtggtaggt attgctaaaa tgggcgatgc caaagccctt ggtcgtattt
     8821 tttctaaaac actcttttta tttatttgcg cctcattgct gtcaattgcc ttaggcttga
     8881 taacggtaaa tttcttcatg ccaggtacag gaattaattt tgttgcacac ggtgccgaaa
     8941 ccaccggagt ggtcgcggca gaacccttta cgctaaaagt atttatttcg catgctttcc
     9001 ccaccagcat tgtcgatgcc atggcgcaca atgaaatttt gcaaatcgtg gtgttctcaa
     9061 ttttcctcgg ctgtagcctg acggcgattg gcgagaaagg cagcgccatc gttcacgcct
     9121 tagattcgct ggcacatgcc atgttaaagc tcactggcta cgtcatgctc ttcgctcccc
     9181 tgaccgtatt cgctgctatt tcagcattga ttgctgaacg aggactggca gttatggtga
     9241 gcgccgggat ctttatgggt gaattttatt tcaccatgtt attactttgg gtactgctta
     9301 tcggtctggc catcgtttat gtcggcccct gcatcagacg cctgacccgc gccctttcgg
     9361 aacccgccct gctggcattt accacatcca gttctgaagc ggcttttccg ggaacgcttg
     9421 aaaaactgga gcaatttggc gtttccccca aaattgccag ctttgtctta cccattggct
     9481 actcatttaa tctcgttgga tcaatggcct actgctcctt cgccacagtt ttcatcgccc
     9541 aggcctgcaa tatccattta tccatcggtg agcaaatcac catgctgttg atcctgatgt
     9601 tgacctcgaa aggaatggct ggcgtaccac gcgcctcaat ggtggttatc gccgccacgc
     9661 tcaaccagtt caatattccg gaagcggggc tgatcttgct gatgggcgtt gatccgttcc
     9721 ttgatatggg gcgttccgcg acaaacgtca tgagcaacgc aatgggcgct gcgatggtga
     9781 gtcggtggga aggcgaacat ttcggcgagg gctgtcgggg taaagcatta aaacccaatg
     9841 aatcgaacgt tgctctgccc tgagggggat cttaagcaat cagaagccaa tgactacgca
     9901 tttttataag ccggatcatg aagttagacg gcaattaagt aacactaacg tttaaggtga
     9961 aaagtgaaat atgtccaaac catttatctg gcaagaaccg tttctacaaa ataaagacgg
    10021 tacagaatat acattaatta gcgatcaaca tattacagta acggaattag acggcgaaga
    10081 agtgataaaa atcgctcctg aagcattaac gctgcttgcc cggcaggcat tttatgaagc
    10141 ctcttttttc ttacgttccg ctcatttgca acaggtcgcc agcattctta acgatcagca
    10201 ggccagcagt aatgataaat acgttgcgtt gcagttatta cgtaatgctg aagtctcagc
    10261 aaaaggggta ttacccaact gtcaggatac cggcaccgca actattgttg ccagtaaagg
    10321 gcaacagata tggaccggag gtaacgacgc cgaagcctta agtaaaggca tttacagtac
    10381 tttccaggaa aataatctgc gcttttcaca aaatgcgccg ctggatatgt ataccgaagt
    10441 caatacgcaa accaatctcc cggcgcagat tgatatcagc gctgtcgctg gtgatgagta
    10501 ccattttctc tgtgttaata aagggggcgg ctccgccaat aaagccgcgc tttatcagga
    10561 aaccaaatcc ttattgcaac cagaaaaact gaccgcattt ttaatcgaaa aaatgaaatc
    10621 gctgggcacc gctgcctgcc cgccttacca catcgccttt gtcgtcggag ggctttcagc
    10681 cgatcaaacc ttaaaaattg ccaagctggc ctcaacgaaa tattacgaca atttgcccac
    10741 cagcggaaac gagcagggac aagcattccg tgatattgag ctggaaaaag tgttgctcga
    10801 ggccagtcag caatttggta ttggtgcgca atttgggggt aaatatttcg ctcatgatat
    10861 tcgcgtcatt cgtctgccgc gtcacggtgg ctcttgcccg atagctatgg cgctctcctg
    10921 ttctgccgat cgcaatatca aagcaaaaat taacaaacac ggtatctggc tggaaaaact
    10981 cgaacacaat ccggggcaat atattccagc ctcactgcgg gaagaaaatc acgcgcaaca
    11041 tgtacaactg gatctaaacc gtccgttgcg tgacgtgatg caggatctcg ccagattacc
    11101 agtaggcacc cgcgtgtcgt taagtggtcc catcgtagtg gcgcgcgata tcgcccacgc
    11161 caaaataaaa gcgcggctgg atagcggcga accgatgccg gagtacctga agcatcatat
    11221 tgtctattac gctggcccgg cgaaaacacc ggagaacatg gcctgcggtt cactgggtcc
    11281 aaccacgggc ggtcgaatgg acggttatat cgataccttc caggcggctg gcggcagtct
    11341 agtgatgtta tcgaaaggta accgcagcca gcaggtcacc gatgcctgtc acaaacatgg
    11401 cggcttcaat ctgggaagta ttggcggtgc agctgcgtta ctggcgcaag aatatgtcaa
    11461 aagtctgcgt tgcctggaat acccggagct gggtatggaa gcggtgtgga tgatggaagt
    11521 ggaaaatctg cccgcattca tcctggtaga tgataaaggc aataatttct ttagtcagtt
    11581 tgagcagcaa caccgctgcg ccagttgccc tgcgggacat taaggaaaat gaatggaagc
    11641 gcgaatttca actgaacggc atgtgcagcg ccaacagaaa ttgaaggaac aggtcgatac
    11701 gcgcgtcgcg gcggcgatag agaaaaaagg gatcttgatt gttttcaccg gcaacggcaa
    11761 agggaaatcc actgccgcgt ttggtactgt gacgcgcgcg gtgggtcatg gcaaaacggt
    11821 gggggttgcg cagtacatca aggggcaatg ggacaacggc gaatacaacc ttctccagcc
    11881 gctgggggtt gagtttcata ttatgggaac cggttttacc tgggagacac agaaccggca
    11941 agcggatatc gacgcggcga aagaggtctg gtcggaaagc aaacggatgc tggctgacaa
    12001 gcgttacgac ttagtggtgc ttgatgagct gacctatatg ctggcttacc attatctgga
    12061 taccgaagaa gtgatcgctt ctctgcaaaa ccgcccggca caacaaagtg tcattgtcac
    12121 cggacgcggt tgccacagtc agatattaaa aatggcagat acggtcagcg aaatccgccc
    12181 cgtcaaacat gcttttgata acggcattca ggcccagccg ggtatcgact ggtaataact
    12241 gcgcctggca tcccgccagg cgcaggtttt acatctcctg cggtacgacc gatttcgcca
    12301 gcaaccattc acgaaattgc tgcaagtgcg gtgagtcaga cttctcttcc tgccacgtca
    12361 taataaaacg attaccggta cgaataggca catcacaggg aatcaccata tcgccactat
    12421 ccaaatcatg ttgaatagcg aaacgtggta acagcgccac gccgagatta gagcgcacgg
    12481 cggcaatcag cattgaaagc aaatcgaaac gtggtccgtt gttgaccagc ggactgctaa
    12541 cgccagataa agcaaaccac tcttcccagc cagtaatacg ggtgctttga tgcagaagtg
    12601 gtaactcagt aagcagttct gccactgaca gtttttgatc ggacattgca agcaaactgc
    12661 cgctgcatac cggcaaaatc tcctcttcaa agagatattc tacttccgcc cagggcgcgc
    12721 aaaaattttc gcgcataatg acagcatcgt actcccgatt aagaaaatcg ccgttattag
    12781 ctaaagaatg aatattaaca ataatatcgg gatgtaactt tgtgaattca tgtaaatttg
    12841 ggattaacca gtgggtacta aaagtaggat taacggcaag ttcaataacc tgaaccgtag
    12901 gctgccaggt cataatggta ttggtatcgc gttccagctt atttaatgtt tcttttacta
    12961 tccccaggta atacttccct gtatcattaa gaaaaatgcg ttttttagag tgtgaaaata
    13021 acggagtacg taaaaattcc tccagcgcac ttacttgtct gaatactgca ctttgggtta
    13081 acgctaactc ctctgccgct ctggtataac tttcatgacg tgcgacgacc tcaaaagtca
    13141 ccagcagttc cgttttaggt attttccctc gcataatgcc gtcccttaaa aatagtttaa
    13201 accatcaaaa atacattact tattacgaag taatggtaac caaagcagca ataaataatt
    13261 tataagaatc atgattgaca tttataaacc agtaaggctg gcgtgacaaa tatcttaatc
    13321 agataataat aatccgaaaa gcacagcgcc tttcgaatta tccggaatca cattaagtga
    13381 aaattattaa attgatatca tttatcatat ctgtggatat tatgataaac aatcaacaaa
    13441 gattttattt ttgatttata gcatatccct ctaagaattt ttgcgatgct gcaatatccg
    13501 gggtaaagac ccgatcctgg ctgacaaatg gcacgacttg acgaaaagcg gtgaacatcg
    13561 cttttgttga tttacttaac gctacggtca aacgctggga agccagtgca tcaggtaacg
    13621 attcgaaatt cgccgtcgtt cttattcgtg gcttgcggtc ggttgcggca atccacagtg
    13681 gctgtggtca tgcagtcaag cctgcagtca gatttcgacc gtggttaacc gtttaaggca
    13741 taaaaaaggc catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct
    13801 gtagagggaa gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca
    13861 gtgcttcacc gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag
    13921 cgaatttctc atccgcagtc cctttcccac cggcaatgat ggcacccgcg tggcccatac
    13981 gtttgccttt cggcgcagtc acaccagcga tgtaacccac aactggcttg gtaacgtgct
    14041 ctttgatgta cgcagctgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga
    14101 tcgcttcggt ctgcggatct ttttcgaaca tttcgagaat gtcgataaag ttagagccag
    14161 ggatcgggtc accgccgata ccgacacagg tcgactgacc gaaaccgtaa tccgtggtct
    14221 gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt
    14281 tgtgaatgtg accaggctga ataccgattt tgcattcacc cggagtgata acgcctgggc
    14341 agttcgggcc gatcatacga acgcctgctt catccagctt cactttcacg gtcagcatat
    14401 ccagcgtcgg gatgccttca gtgatggtga taatcagttt gatgcctgcg tcgatggctt
    14461 ccagaatgga gtctttgcag aacggtgctg gtacgtagat aacagaagcg gtagcgccag
    14521 tagcagcaac agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtaccgc
    14581 ctttacctgg ggttacgccg ccaaccattt tagtgccgta tgcaatggcc tgttctgagt
    14641 ggaaagtccc ctggctaccg gtaaagccct ggcagataac cttggtgttt ttatcaatta
    14701 aaatggacat tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc
    14761 ttttgctgca ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt
    14821 gttaccttcc agacgtacca cgaccggtac gttaacaccc acttccgcta ccgcaccgat
    14881 gataccgtca gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc
    14941 tttcactttg tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc
    15001 gccgccgcca acgtccagga agttagccgg ttcgccgccg tgcagtttaa cgatgtccat
    15061 cgtacccatc gccaggcctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac
    15121 gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg
    15181 catttcgcgc agatcaggct ggcggaacag ggcgttgcca tcagcgccca gtttgccgtc
    15241 gaggcaaatc agatcgccct gtttggtgat gaccagcggg ttgatttcga tcagcgccag
    15301 gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag
    15361 tttaccttcc agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt
    15421 cagcggatca agcgcaactt tgtggatcag gtgcggagtt tcttccgcca ctttttcgat
    15481 ttccacgccg ccttcggtgg aggccataaa gaccacgcga cgtgaactac ggtctacaac
    15541 ggcaccgaga tacagctctt tagcgatatc ggtcgctgct tcaaccagaa tctggttaac
    15601 cggttggcca ttggcatctg tttgatacgt taccagacgc ttgcccagcc agttttctgc
    15661 aaaagcacgg atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc
    15721 accagcgtga acctgacatt tcactaccca cggaccggca ccgatttttg aagcggcttc
    15781 ttctgcttcg cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc
    15841 aaacagttgt tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat
    15901 cgttatcttt taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaaa
    15961 cttaaactac tacacgtcca gcagcagacg cgtcggatct tccagcaact ctttgatcgt
    16021 taccaggaag cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag
    16081 gtacatcatc ggcaggatct caacctgacc attcacagcc atcggacgat ctttgatagc
    16141 gtgcataccc agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc
    16201 gaacacacca ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt
    16261 gccgtcacgg cctttaactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag
    16321 ggtatcgaca tcacgcagta ccggcgtcac caggccgcgc ggcgtagaaa ccgccatgct
    16381 gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg atagaagcat tcacttccgg
    16441 gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat
    16501 gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat
    16561 gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc
    16621 tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt tcgctacgtg cagccagagc
    16681 cggttgcgcc gccggagcag ccgctgccgg tgcagactct ttcgccggag ctttcgccag
    16741 atgtttttcc acatcttcac gggtcagacg accacccaca ccggtgcctt taatggcgct
    16801 ggcgtcgagg ttatgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt
    16861 ttgctcttcc agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt
    16921 ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga
    16981 cgttaccgtt gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg
    17041 tacttccagt accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc
    17101 gtcaccgggt tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc
    17161 agggaccaga atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg
    17221 tcattaacca gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag
    17281 gcggaggccg ggcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa
    17341 tgatgctggc tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa
    17401 tccttgacgt gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag
    17461 agttgctcga tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc
    17521 aggtcgtaat aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc
    17581 tcgtcgattt caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa
    17641 accgccagcg gatgacgcag cagggatttc ggcgacatca cgaccagcgg acgacgcatc
    17701 ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggtacgcac
    17761 acctgcatgt tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc
    17821 tccggcccct gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc
    17881 catttctgtt cgccagagga gatgaactgg tcgataacca cctgcgcacc gttggcgaag
    17941 tcaccgaact gagcttccca gatagtcaga gtgcgtggtt ctgcggtggc ataaccgtat
    18001 tcaaacgcca gcactgcttc ttcagaaagt acggagtccc agacacggaa cgcgccctgg
    18061 ccgttatgga tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc
    18121 gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg
    18181 ccttcgtcaa ccagcgtggc gtaagcgagg ttttccgcgc cgccccagtc gaacagtttc
    18241 tcaccggcag ccatcgcctg gcgatcgcca taaattttgg caacgcgaga ctgcatttca
    18301 accgcttccg gcaccgtgct gatgcgtttc gccagctcct gcaggcgctt catctcaact
    18361 ttattcgggt actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc
    18421 atgttcatcg gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac
    18481 aggttaacca tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca
    18541 gcatagattt tgcgcggcgt cggatgtttt ttgattttct gatacatcag cggctgggtt
    18601 gcgctcggct cgtcggcttc gttgtggccg tgacggcggt agcacaccag gtcgatgaag
    18661 acatcacgtt taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct
    18721 tccggatcat ccgcgttaac gtgaaaaatc ggtgcctgaa ccatcttacc gatatcagta
    18781 cagtacggcg ttgaacgggc atccagcggg ttagaggtgg tgaagccaac ctggttgttg
    18841 ataacgatac gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt
    18901 tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt gaatggtgat tggcagcact
    18961 ttgttgctgc tcggctcatc aagtctgtcc agacgggcac gaacagaacc gataacgacc
    19021 gggctgacaa tctcaaggtg agacgggtta aacgccagcg ccagatgtac caggccgcca
    19081 tcggtctgga agtcagacga gaagcccatg tggtatttca cgtcacccgt gccgaggtgt
    19141 tctttatgtt taccggcaaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc
    19201 accagcacgt tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg ggtgccgctg
    19261 ttgccagcgt ggcgaatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc
    19321 gagaagcgtt ttgcgccagg gaattttgca ccgaggtaac gttcaaggcc ttcagcggcg
    19381 gtcagttcgc ttaggaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg
    19441 atacgctgtt ggatccagcg tttttcttcg gtgctggtaa tgtgcatata ctcggcacca
    19501 atcgggccgc agtaggtttg cttgagggct tccagcagct cgccgagttt catggtttct
    19561 ttgccgctgg caaatgaacc gacgttgaag gtctcctgga agtctgcttc ggtcagatcg
    19621 tggaaagacg gatccagatc ggccacttta tcttgctgcc acagtcccag cggatcgaga
    19681 ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga gctgcaggac ttttacctgc
    19741 ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg
    19801 cggcggaaat attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt
    19861 aactgctgga acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct
    19921 tcatagagct gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag
    19981 gctttcaaag cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc
    20041 cgtggatact accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta
    20101 cgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag
    20161 atttcctgca tttctttgtt acgcctgatg cgctgcgctt atcaggccta cgggtaatga
    20221 cttgtaggcc ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcgacgctt
    20281 gcgcgtctta tcaggcctac ggtttacgca ttacgttgca acaacatcga cttgatatgg
    20341 ccgatggcgc gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgcta
    20401 tgacagcgga atacactgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta
    20461 tcacggctat cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta
    20521 tcgggattcc accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca
    20581 tacagcccgt cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga
    20641 ttttgtccat tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc
    20701 atgtctacca ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc
    20761 ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc
    20821 atgttcagac cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg
    20881 ggatctttct ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc
    20941 gcttccaggg tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa
    21001 attgaaaact cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg
    21061 aatgccgggc gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactct
    21121 ggcagataca gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg
    21181 ctatgtgcgc cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt
    21241 tccatcaggt tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg
    21301 tcatccagac gggcattttt cagacgctcg cggatcactt tcaactgctc aagcccttta
    21361 gccatcgcat caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct
    21421 ttacgaatcg ccaccggatc ttcaccgtta cgattattgt tccagcggtt cagacgatcc
    21481 aaagatgctt caacatcaga ctcgctggca tcgcgcaatg cgccctgctc ggcgatagac
    21541 tcttgcagat gcagacccgc tgcgcgacca aagaccacca ggtccagcag cgagttgccg
    21601 cccagacggt tagcaccgtg taccgatacg caggcaattt cacctaccgc gaacagcccc
    21661 ggaacaacca catcttcgcc tttctcattc acagtcagcg cctgaccggt aactttggtc
    21721 ggaataccgc ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga
    21781 tcaacgtgag cgaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact
    21841 tctttaccca ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg
    21901 cgaccttcac ggatttcaat catgattgaa cgcgccacca catcacggcc cgccaggtct
    21961 ttggcgttcg gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg
    22021 ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg
    22081 aattgccaca tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacgccg
    22141 tcgccggtgt taatgtgggc gttagtggta gactgataaa tacgccctgc tccgccagtc
    22201 gccagcacgg tagcgcgggc tttaaaataa accacttcac cggtttcgat gcacagtgcg
    22261 gtacaaccca ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag
    22321 aaaatggtgg tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg
    22381 gtacggtcag ccgctgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga
    22441 ccgccaaacg gacgttgata gatacgacca tcatcgagac gcgagaacgg caggcccatg
    22501 tgttcgagtt ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg
    22561 tcaccgatat agtccgaccc tttcacggtg tcgtacatat gccattccca gttatcttca
    22621 tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc
    22681 gggaagactt tagagagcag cgcacaggtc tggccgctct gggaaatttg cagcgccgcg
    22741 cgcatacctg cgccgccggc accaatcaca actgcatcaa attctctgac tggcaatttc
    22801 atcacacacc ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa
    22861 tcaccagttg cagcatcagg cgcaaagcca gcggtttaac gtagtcggtc aacacctgcc
    22921 acatgccgat ccaggcatgg atcaagatag aaaacagcgc cagcagggtg aacactttgg
    22981 tgaacgcaga ggcgcagaaa ccgatccaga cttcatatgt cagctcgcca ctggtggcga
    23041 aaaaaccgac catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga
    23101 tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc
    23161 tgcgagaagt gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc
    23221 ttcgaatgtt tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac
    23281 gtgatacgcc agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat
    23341 aatcgcggaa gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccacag
    23401 caggataccc actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat
    23461 cgccgtgatg gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac
    23521 atttcttatc atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca
    23581 cagaggttaa ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg
    23641 ggtcagacag cgtcctttct ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa
    23701 aacgacacgt tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc
    23761 agtataggcc gttcacaaaa tcattacaat taacctacat atagtttgtc ggg
//
LOCUS       DAZFTN010000072        23751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFTN010000072 DAZFTN010000000
VERSION     DAZFTN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..673
                     /locus_tag="ACHKV2_003161"
     CDS             <1..673
                     /locus_tag="ACHKV2_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941002.1"
                     /translation="DLFRGISSNYRVVVQLLISSCLSGDSDTIYDIQEELPFSWGWIP
                     VSIWKDVFQKCWTYLEKQINDKTLALHILQPFIAFMNHRAHIDRRLAPIANMLLTYSE
                     SLPTGCDVLPTVSREQFNEAKQMLLRNPDSFGRISIFPKELWSSAITPELKSVFNKLW
                     IEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQYYFEQ
                     AGVCH"
     gene            673..6987
                     /locus_tag="ACHKV2_003162"
                     /pseudo
     CDS             673..6987
                     /locus_tag="ACHKV2_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            7211..10069
                     /locus_tag="ACHKV2_003163"
     CDS             7211..10069
                     /locus_tag="ACHKV2_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6941003.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKKQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            10072..15006
                     /locus_tag="ACHKV2_003164"
     CDS             10072..15006
                     /locus_tag="ACHKV2_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941004.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            15006..21347
                     /locus_tag="ACHKV2_003165"
     CDS             15006..21347
                     /locus_tag="ACHKV2_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6941005.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            21344..23458
                     /locus_tag="ACHKV2_003166"
     CDS             21344..23458
                     /locus_tag="ACHKV2_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO6941006.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
     gene            complement(23568..>23751)
                     /locus_tag="ACHKV2_003167"
     CDS             complement(23568..>23751)
                     /locus_tag="ACHKV2_003167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941007.1"
                     /translation="YHHHGSRDYWSAGNASLFVPAIFVQCVTPSKNHPHPDVTLDIEK
                     TGCTVQYANRISEYNF"
BASE COUNT         6022 a         5634 c         6347 g         5748 t
ORIGIN      
        1 ggacttgttc agaggtattt cgagtaatta ccgcgtggtt gttcaattgt taatatcatc
       61 atgtctttct ggtgatagcg atacgattta tgatatacag gaagaattac ccttttcatg
      121 gggatggatt cccgtttcaa tctggaaaga tgttttccaa aaatgttgga cttatctgga
      181 gaaacagatt aacgataaaa cattagcatt acatatattg caacccttta ttgcttttat
      241 gaaccatcgt gcacatatcg atcgtcggct ggctccgatt gcgaatatgt tacttacata
      301 tagtgagagc ctaccaaccg gttgtgatgt attgccaact gttagtcgtg agcagtttaa
      361 tgaagctaaa cagatgctat taaggaaccc cgacagcttt gggcgtatca gtatcttccc
      421 taaagaactt tggtctagtg ctattactcc agagttaaaa tctgttttta ataagctttg
      481 gattgaagat aaatatcact cacggcttga aaaacgtttt aatttgatgt tagtcgcagc
      541 gctgttaacc caaaaagata ataacctgat acatcaactg tctgcgcttt ttgaatttca
      601 ctatcagcaa gccccacagc aattaggggt aatctatcaa tattattttg aacaagcagg
      661 agtatgtcat tgatggacgt tcagcaactg cattatgcgc cacttctaaa tcagcttcat
      721 cagcgtgcgg tgaggtcggt ggtaagtcag ttggcattgc ggagcaagtc gttgactcat
      781 tatctggaat cacaatattt tcagcctccc ggtcaggagg ggtcactgct tgcagacccg
      841 gtattcgaaa gtacattcgg ctggatgcct gcggatgaaa caatggagga tttgcgggac
      901 aagctgatct ccagcccgct ggttgatgca ttgagcaaag cgccagtgcc atttcgtcat
      961 caacttactg cgtggcgaac gattctggaa gacaaaaaat ccttgttagt gagcagcggt
     1021 acgggatccg ggaaaacaga atgctttatg gtaccggttc tggaagatct tattcgtcag
     1081 caaaaatcga cccgccagtc actaacaggg acacaagcta ttttcttgta tccgttaaat
     1141 gcattaatta acagccaaca ggagcggctt cgcgaatgga cgcgcggatt taaggggaaa
     1201 atacgcttcg cgctatataa tggcgaaacc cggcatacca aatacgaaat tcaggaagat
     1261 cagttgaaag tacctgaaca agctttgagt cgggaagcca tttatgaagc accaccttcc
     1321 atcatggtga cgaataccac catgctggaa tatatgctga ttcgtcgtaa agatgcccca
     1381 atcatcgaaa agtctcaggg gatgttaaag tatatcgtcc tggatgaagc ccatagttat
     1441 attggttctc aggctgctga gttagcgctg cttttacgtc gtgttatgca ggctttcaat
     1501 gttggacccg gcacagataa acctgtgcag attatcgcga cctctgccac tattggtgaa
     1561 gactctcctg aaggtaataa ggagcttgct aaatttgtcg ctgatctcgc gggcgttaca
     1621 gaacgtgatg tcaaagtggt tcgcggttat cgacaaatac cgcgggtatc ggagtcgctg
     1681 attcgtcatg aataccccac atctttaact tctcttaaat ccctgagccc tcaggatctt
     1741 taccaacagc tttgtcatta ccgtgtggcc cagcaattgc gtcaggcgtt aactcatccc
     1801 ggacggcagg cagtacgttt gagtgagttg ctcaatgttg ccagaagaac ctggccagat
     1861 attaatcacc gtgagctact gcaactcctc gatctaatgg caagatcgcg agaaggtgaa
     1921 ctggctttta cgccattacg tatgcatggg tttatcagaa cactggcggg tctatgggca
     1981 tgttcaaaca aacaatgctc acataaagca catgaattaa atcagagtga ctggccattt
     2041 ggtcaggtat ggtttgagca gcgccaatat tgtgactgtg gagcgccggt atttgaagtt
     2101 ttacgttgct ccgggtgtgg ttcagcttat ttgtctgcca aagaggagat gcgcggtgat
     2161 ggaacgaact ggttggtagc gcaaccagcc gctgccgaag tggatgagtt tgctcttgat
     2221 gtagatgttt attctgaaga tgaagacaac gacgaattag acaataactc tcaatttgat
     2281 cgtctgattg cgagcgatgg tgaaaaatat atcatttcac tggaagaaac gacggcgggg
     2341 aaaattgata gtgaagcgca aaagcactat gaaataaacc tgttaagacc ggaatctcga
     2401 ggtgagggac gcaataactc ctttgcttgt gtttgctgtg gtgacactca acggaaaaat
     2461 aatcctttat tccgtccgct tcggctcggc gcgccctttt ttttaaatga aattattcct
     2521 actctgctgg agttttctcc attaccgcaa acaagagaag agcagttagg accgtttaat
     2581 ggtcgacgct tgctcacctt tacggattcg cgtcagggta ccgctcgcat ttcagcccgc
     2641 ttacagcagg atgccgataa agcgacgttg cgagctcttt gttactagga gctggcaaat
     2701 attacagagc cttcagcaaa actcagcccg gcacactgtg cactgttaga gaaagttatt
     2761 agccagtttt ctgtcctccc tttaaaaaat tccatcgacg cgctgaaaaa agcgatggct
     2821 gcgaatgagg agcttagccc gacggacatt caggcactta aaattattga gccgatggtg
     2881 agttccttaa gcacggagca tgctgaagcg atgcaaatac ttcaggctaa ctctggcaat
     2941 cagcagaaga aaaccatgtc atggcaaacg ctgtctgaac agctggttgg cagtgtggac
     3001 cttgccgata gaatgagagg cagttttaaa gaattgtctg ggcttgatct tactaaacaa
     3061 cagtttgcag atttttgttt atacagcgag tttggtcgcc gccctaaaaa tgcctggacc
     3121 ctcgaaagtc tgggtctggt agccattcac tatccattca ttgataaagt tacaacctgc
     3181 ccgcaggact ggaagacatt attacccgat cccgataagc aattagcgga atggcgtaat
     3241 ttattgaaga ttactcttga tttctttatc cgtgaaaaca gtgccgtttt ttacgaaaac
     3301 gagcaatacc ctcgctggat gggggcacgt ttcccggtaa aaatgctcca ggggccagat
     3361 aaaaaaaaca aagggcagaa acgtgatcaa ctttggccac aaatacgcga tcgccattat
     3421 aaccgggtca ttaagctatt aatcgcagtc tttccggcaa tacaacctga gcaggctcac
     3481 tggaaatcgt tagttaacca cttaatgatt gaggtctggg atgcaattcg accctgctta
     3541 cgtcagtttg agtcgggtta tcagctggat attaaacaac aagctgaatt ttacagtcca
     3601 aagcaagtgt ggcgttgccc ctatactcga cgtgcgttag acgtcacgtt gctgggatac
     3661 tctccgtatt tgccgggtag caaagaaatt gcacctgaaa aagcagtgtt gattgaaatg
     3721 cctgagcttc ctgtacgtca ctggcgtttg tctggtggtg gagaaatcgc ccgagaagaa
     3781 cgtctggagt ggctggaaag taatgcgctg attcaacatg cccgcgagga aggattatgg
     3841 tctactcgca gcgaccgact ggccctgaaa gatagctggt atcgtctgga ggaacactct
     3901 gcgcagagaa cgcctgagca gaaccagttt aatgaaaaac aatttaagtc aggcaaagtt
     3961 aatgtgctga actgttccac gaccatggag atgggggtgg atattggtgg tatgagtttg
     4021 gtggcaatga acaacgttcc tccagcgccg gcaaattatt tgcaacgtgc aggtcgcgcg
     4081 gggcgtcgtg gagagagtgc atctgctgcc attacgttat gtaaaaacac ggcgcatgga
     4141 atggaggtat tcaaagaccc cctctgggcg ttcaacacta ctgccagtgc gccacgggtt
     4201 cgttttggga gcagtagtat tgttcaacga catgttaatg ccctggtttt aggtctgttt
     4261 ttacgagctg aagttcctga tgcgacaaaa ctgagttgca aatggttctt tgaaggtgat
     4321 gagagccagt gtcttcgttt tctccattgg ttaaatcacc aggccgatca gctggcggat
     4381 aaacttaaac gccttactca gggaaccgta ctgatgtcat tgacggcaac ccagctcttg
     4441 acccgcacac aagcaatgat gcagcaggtt gatatccgct ggagaagcca acttgcgata
     4501 ctgttggaaa atatcgaagc gttgaaggcg gataacagcg cgtgggaaga aacaccagca
     4561 ggtaaagcca tcgcttatca gcttcgtgat tatcgcggtg catatctgtt ttctaaactc
     4621 attagtgaag cgttcctgcc ggggcatggt ttcccggttg gcgtagtcaa tttcaattat
     4681 ctgacggcag atgagctgga aaaacgtcgc gcaataaaag ccactcaggc tgaccctaat
     4741 gaagggggag aaagcttttc tcgtcgtatt gaaaaattgc ccagccgtga tttgcctaca
     4801 gcattacgcg agtatgcgcc tggtgcagat gtggtattgg gcggaaaagt atatcgttcc
     4861 tcgggcatta tgcttggcaa ggtgcttgcc agcgggcagg agctgtcagg cgatcatcat
     4921 attccgtggt tctggcattg ccgtaaatgt ggtgcggggg caaccagtac tacccatcct
     4981 gtcgaatgta gtcactgcaa agcggatata cagcaacttg atgtgaagcg ttatttacag
     5041 cctgttggtt ttgcgacaga tattcgttat caggcacata atgatgtatc catgcctgca
     5101 caacttccct ggaaagatcc acgtgttctt gtaccgtctt cggtgtgggt ttctttaccc
     5161 gatgctgggc taggacgtta ccgttttagc catagtgggg aattgttcca cttcagtgaa
     5221 ggggaatttg ggcacggcta cgcaatttgc ctctcatgtg gtcgggcaga atctcaaact
     5281 caaccccagc gtacccctga aaatctaaag aacccagaga gggaaaatac gcattacctg
     5341 ttgcgagggg gcagtaacga tcgtcagggg agcaataaat tgtgccacgg tcatgtgcat
     5401 aaagatctgt ggttgggata tagcagtcgt acagatatgg ttgagctgca actcaatgat
     5461 gataatggcc tgttgatccg tgatgaggtt gccgcgcgtt cccttgcggt cgctttgcgt
     5521 gaagggttag cgcataaatt gggtatcgaa aatactgagc tgggcgtgac gacgcagcaa
     5581 gcgcgcgata tcaatggcta taccggatat tccatcttca tttacgacaa taatgcgggt
     5641 ggtgcaggtt atgcagtgca attgatcgac cattgggctg atgtctttaa ttacgccaga
     5701 aaattgctcg attgcagttg cgataaattc tgtcaccact gcttgctcag ctacgacagt
     5761 cagcattatg tcaacagatt agatcgtcac catgcattaa ccctgcttac caatgtacgg
     5821 ttgcagcgac ttaacctcgc gccagaatat cagtacttcg gcgatggtag ccgggttgaa
     5881 actaaccctc tgagtttacg tattgcgcaa tgcctgaaca gcgagattta tgactcctgc
     5941 tcgctggttc ttgcggggcc gcaggaacag tgggactttg cccaatggcc attatttaaa
     6001 gagttgctcc agtttgcctc ctccggtggg aatgttgagt tgctggtggc cacgccgctg
     6061 gcaaacttaa ctgatagctc cagacatcag ctttccgcgt tggcggcgat gccgggaggt
     6121 cgcttacaag tgaaatccat tgccactgcg cagctcatgc aaggaaaggg gcgctggctt
     6181 gcgcaggtga ctcgcgaagg gcaaagccaa cagtgggccg cagacgacag cgctaccgtt
     6241 gcacctggtg aattatgggg acaaagtgcg tcttcaccag tagtgacact taaaggaacg
     6301 tccggaaaaa ccttctcggg tcagacctta agtgcagaag atttactgcc cgccttaccc
     6361 accggggcgg tgcgcatcaa tctctgtgaa caactggacg ggccgctgga aggttttggt
     6421 tcgcgtttct ggtcgttagt cacgcaacag cacgctggct ggaagcaggc atttacccgc
     6481 cacaaagaga taacgcatgt tgagtacagc gatcgttatc ttaatagccc attcacggcg
     6541 cgtttgttag gtgaaatctt aaccgagctg gttgaacagg gaatggcgga acgcgccagc
     6601 ctcactgttt gcgtcaaaaa attggattac aactctcgcc aacacgacgc tctctataac
     6661 gcgtggctta atgaggaaga tcgtcagcag gttgtgacca ctttacttga agaaggatat
     6721 ctggggcctg cgtggccggg agcaattagc tggttaacgg gggataacca gagtacggag
     6781 catggacgag aactgacagt caccttcagc gatggcagtc agcattatgt cctgctcgac
     6841 atggggctta gctactggcg ctgtattgaa gataccttct ttgattttgc cctgcgagta
     6901 ccgcagcaag ttgaacggct ggcaaacacg cgagcgcgag ctgttgcccc gggtaatgac
     6961 cttaggagtt atattatcgc tggataaata attgctcccc tggggatatc ttccagggga
     7021 gttgacccag ctcacttttt aataattttt gtttgaaaat catgcgcaat ttatgcgcat
     7081 tccctcccat tttgtcactt tgccgggggc cttcatgata tctttaaggc cctctttttt
     7141 atcgcagtgt gccgcgccac atttttcagg atgacgttac cgccactgcc ccaaatagac
     7201 agaaaaaccg atgaataagc aaaattatgc acctggtatg cgggtggtta ttcgtgatgc
     7261 cgaatggcgt attcgccggg cggatgacag tggtgatggt gggtatttgc tgacctgcga
     7321 tggtatttca gagcttgtgc gcggtaaaga agggttattt ctgaccaaac tggaacaaaa
     7381 agtagagatc ctggatcccg caaaaacgca tttggtggaa gatgaatccg ccaattatca
     7441 agcggcacag ttgtatattg aaagtcagtt acgccaacgc gtaccgacag acagtaaagt
     7501 ccattttggt catctggcgg cgatggattc catgccgttt cagctcgatc ctacgcggat
     7561 ggcgctggca caaccgcgtc agcggatact cattgccgac gctgttggtc tgggtaaaac
     7621 gctggaagcc ggtattctgg tttcggaact gatccgtcgt gggcgcggaa aacgtattct
     7681 ggttctggcg gttaaatcga tgctgacaca attccagaag gagttctgga gccgttttgc
     7741 gatcccattg acccgcctgg attctgctgg tttacaaaag gtgcgtaacc gcatcccaac
     7801 caaccacaac ccgttccact attttgacaa gaccattatc tccatcgata cgctgaaaca
     7861 agacattgaa tatcgtcacc atctggaaaa tgcctggtgg gatattattg tcattgatga
     7921 ggcccataac gtcgcagaac gtggaaccag ttcgttacgc agtaaactgg caaaactgct
     7981 cgccgggcgc agcgatacgc tgattatgct ttcagcaaca ccgcatgatg gtaaggctga
     8041 aagttttgcc agcctgatga acatgcttga cccgacggcg atcgccaacc cgaaagagta
     8101 tgaatacgcc gattttgccg ataaaaatct ggttgtccgt cgcttcaaaa aagacgtgaa
     8161 agatcagatg tccggcgagt tccccgagcg caatattgtc aaattgactc gcctggcatc
     8221 cggtgccgaa gaagaagcat accgtcgtct ggttgagagc cagttccgtg atgatgacga
     8281 tgaacaggcc cagtctaaca aaggtcgtct gttcaagata accctcgaaa aagcgctatt
     8341 ttccagcccg atggcgtgtg ccagcgtcgt ggcgaatcgt ctgaagcgtc ttgagagccg
     8401 taaagatcat aatagccaga gccagatcaa cgaactggag tcattgctgt tggcgctgaa
     8461 taacattgat gccagccagt ttagcaaata ccagttgctg ctcgacacca tccgaaaaga
     8521 cctcgcctgg aaagccaata acacggaaga tcgcctggtg atcttcaccg aaagtattaa
     8581 aacgctggag ttcctcgaac agcaactgcg agcggatctg aaattgaaag atgaccagat
     8641 cgccacgctg cgcggcgatc agggcgatac ggtattgatg gaaaccgtag aagcctttgg
     8701 taaaaagcag tcgccgttgc gtcttctggt ttgttcggat gtggcatctg aagggatcaa
     8761 cctgcatcac cttagccaca aaatgattca ctttgatatt ccgtggtcgt tgatggtatt
     8821 ccagcaacgt aacgggcgta ttgaccgcta tgggcaaaaa catcagcccc aaatccgcta
     8881 tctgcttacc gaggccagcg aaccacagat caatggcgat atgcgcgtac tggaagtgtt
     8941 gatcaacaaa gacgaacagg cgcagaagaa cattggggac agttcggagt ttaccggcaa
     9001 atttacccag gaagaggaag aagagcaggt tgcagagttc atgatgcagg atgatggtgc
     9061 cagcctgttt gatcaactgc tgaacagcaa cgtctcggaa agcgccgaac acgatctgtt
     9121 tggcgaaata tgcagtgcgg tttccagcga tgcctcaatg gtcaccgaaa cagacaccag
     9181 cttgtttgcc agcgaacagg cgtactgcga aagagcattg ggttacctga aagccagtgg
     9241 tcaaactatc cagtatgaaa ccttgcctga taataccttg tcgctggtgg caccggagga
     9301 gttacgccgc cgcttcaacc agctaccgcc tgaaattgcc ccggagaact ggcagctcta
     9361 tttaagtcag gataaaaccg tcatcaccga cgcgattgcg cgcgctcgcg gtgagcaaca
     9421 tgcctggccc gatgtgcagt atctctggca aatcaacccg gtagtgcagt ggctggacga
     9481 taaaatctct tcggcttttg gtcgtcatca ggctccggtt atccggctgc catacctgct
     9541 tgaacccgat gaagatcact tcattctttc cgggttattc ccgaaccgta aatcacatcc
     9601 gatggtgaac ccgtggatag tggtgagctt taaccgtgaa tcgctaatcg gcagtcagcc
     9661 tttcgctgag tttttacaac gtcatccgca gttgagtaac aagctgacta acagcggcgg
     9721 taaagatcgt aatcaccaac gccagcagga tttactggaa gcggctattg ctcatgcccg
     9781 ggaggtattc atccatgatc ggaatgcgtt tgaaacgcac attaaccagc aactgaatga
     9841 gcatctgcaa aagctggacg ttttgcgtgg gcggcagttg agccaacttg agctggattt
     9901 tgccgataac aaacagcaat tgtcagtcaa gcagagccgt aaagagcaga gacaacgcga
     9961 aatcgaacac aattttgaca gctatatcga atggattgag gacaccatga cgactgaaaa
    10021 agaaccctac attcaggtaa ttgctgttat caccggagcg gagggttaat catggcgctg
    10081 gtcggtatta ataacgaaaa cgaattttac tctaaccact atttgggtga ggttttcacc
    10141 agtgatatcc gcgatgtgct ggaaccctgg atagcccagg aaaatgcagc gcgtgaagcg
    10201 gagcgtgccg ctcgtgaaca gggcaaagac gtggagccgg gataccgcgc tccgtggaac
    10261 cagtttaaca gtctggcgac tgagtttttc cgcaaacttg ccgagcacga aaaacagcgt
    10321 cagatcccgc agcgtctggc cgatcaacgt aatcgctggc agccattgtt aaaggcgctg
    10381 ggctacgaaa ttacgccaca gatccaaatg ctggatgacg atacaccgct gccggtactg
    10441 gcgcgttaca acagcactga cggtagcccg tggctgtgga ttgttgaagc acacgatcag
    10501 gaagaaggaa cgctggatcc gctggcgctc tccttactga ccgcgcaatt cccggcggat
    10561 accgacaaac ataagcacga cagcctgcgc aaaaaagcca acggtgaata tcgcagctgg
    10621 caggatttgc tctctacggc ggtcttcacc caaaatgaac cgccgcgttt tgtgctgctg
    10681 ctcggtaacc gtcagctatt gttgttggac cgtactaagt gggcgcaaaa ccgtctgcta
    10741 cgttttgatt ttgaagagat tttaagccgt cgtgaaacgg atacgctgaa agcgactgcg
    10801 gtgttgttac ataaagattc tctgctgccg ggcagtgggg caccttatct tgactcgctg
    10861 gatgacaatt cgcacaaaca tgcgtttggt gtttcggaag atctgaaata tgccctgcgc
    10921 gaaagcattg agttgctggg caacgaagcg atgcattatc tgatcgaccg tggcctggca
    10981 aactatacag gtaatcgtgc ggtggacccg gatgaactga gccgcgaatg tctgcgttac
    11041 atgtaccgcc tgctgttcct gttctacatt gaagcgcgcc cggagctggg ttatgcgcca
    11101 atgaccgcca aaacctatct gcaaggttac agcctggaaa cgttgcgcga tctggagatg
    11161 atcccgctga ccagcgaaga agatcgcaac gggcgctact tccacgacag cctgaatatg
    11221 ctgtttaaac tggtgcgcga aggctacaac ggcggcgtga aaatgcagag tgacctggag
    11281 agcggcgacc ggatcaccat ccatagtcat cagttcagcg tcccgcgtct ggaaagtcat
    11341 ctgtttgatg ccaacaacac gcgcattctt aaccgcgtgg tattccgtaa cgaaaccctg
    11401 caacagatta tccaggcgat gtcgttaagc cgcccggcca aagggcgctt taaccgccgc
    11461 ggacgtattt cttatcgcca gttgggtatc aaccagttgg gtgcggtgta tgaggcgctg
    11521 ctctcctatc gcggattctt cgccagcgaa gatctctacg aggtgaagaa agccggggaa
    11581 gagtttaacg agctggagac gggttacttc gtcagtaagg atgagattag caaataccac
    11641 gaagacgaga aggtctacga gaaagacggc agtctgcgca ttcaccgcaa aggcagcttt
    11701 atctaccgta tggccgggcg cgaccgtgag aaatctgcct cttattacac cccggaagtg
    11761 ctgacccgct cactggttaa atatgccctg aaagaactgt ttaaggagca gattgatccc
    11821 attagcgatc cgcacgccaa agctgatgcc atcttaaacc tcaccgtgtg cgaaccggcg
    11881 atgggcagcg cggcgttcct taacgaagcc atcaaccagc tggcggaagc gtatctgttc
    11941 cacaagcagc aggcggaagg tcgccgtatt ccgcaggatc gttacaccca ggagttacag
    12001 cgggtgaaaa tgtacattgc cgacaacaac gttttcggcg tggacttaaa cccggtggcg
    12061 gtggaactgg cggaagtgtc gctgtggctg aacgccatta gtggcgatgc gtttgtaccg
    12121 tggtttggtt accagctgca ctgcggtaac tcgctggtgg gcgcgcgccg tcaggtgttc
    12181 aacaagagcg aactgaccta caaaaaagcc aaagatccga gctggcttaa cagcgagccg
    12241 gtcgaactgg cgatgaacac gccgcgtgaa gagacgcaga ttttccactt cctgctgccc
    12301 gacggcggta tggctaacta cagcgataaa actgttaagc agcgttatcc ggatgacttc
    12361 aaagcgctgg acagctggcg caaagagttt attaaaagct ttgccgggca tgagattgct
    12421 gatgtgcagc gtatcagcga aaaggtggaa gcactgtgga acacctatcg ccagcaactt
    12481 aaagcagaac gtctgaaaac cgccgacagc tacccggtgt ggccggcaga aaacagcgag
    12541 cagacgcgtt cttcgctgag cagtaaagat gaaaccttta gcggtcgtct tgaagataac
    12601 agcgcctacc agaagctgcg ttgggtaatg gactactggt gcgcgctgtg gttctggccg
    12661 atcgacaaag ccgatgagct accggatcgc ggcacctggt tgtttgagat tgaaaccctg
    12721 cttgacggga ttgtaatcac ggaaaaagtc actgaagttg cggagcacac caccggcgat
    12781 ctgtttgccg aagaaggcct gctgcgagaa gagtcttcgc tgttttctgt tgctggtcgt
    12841 ctgaaaaccg aggtgttgtt ccgtcatttg ccgcgtctgg cgattgtcga tgccctgaga
    12901 aagcagcacc gtttcttcca ctgggatctg gagttctgcg acctgtttgc cgagcgcggc
    12961 ggttttgacc tgatgctcgg aaacccgccg tggctgaaag tggaatggca ggaagcgggc
    13021 gtgctgggtg attacgagcc ggaatttgtg ctgcgtaagc tgagcgcctc gaagctggca
    13081 acgttgcgta ttgatacctt taaccagatc ccggcgctgg aagcggcctg gcgcagcgag
    13141 tatgaaggct gtgaagggat gcaaaacttc ctgaatgcgc agcagaacta cccggtactg
    13201 cgcggggtgc agaccaactt gtataaatgc tttctgccgc aggcctggcg attaggggcg
    13261 cagaaaggcg tggcaggttt cctgcacccg gaagggattt atgatgaccc gaaaggcggg
    13321 caattacgtg cggcggtata tccgaggctg agggcgcatt ttcagtttca gaatgagtta
    13381 aatttgtttg ttgaagttga tcaccatgcg aagtttagca gcaatattta ttctgctagc
    13441 cctagcacag tgggatttga acatatatct aatttgtatg ctccgcaaac tattgatgca
    13501 tgttttgaac attctggcag tggggacatt cccggtctca aagacgagat tgagagcgag
    13561 ggaaaattaa aagttgtatg gaacacatct ggccaccgtt ctcgattaat aagtatcgcc
    13621 actcatgagc tagaattatt tgctcgtcta tatgacagcg aaggaacgcc agcctggcag
    13681 gcacgtttgc cagccttaca tgctaaacaa cttgttgctg tactggaaaa gtttgctaat
    13741 cagccgaata gattaggtga tttgcagggg cagtattttt caacggttat gttcgatgaa
    13801 acatatgctc agagggatgg gacaatttta cggcagactc aattccctca agattcatca
    13861 caatgggtac tgtctggccc tcatttcttt gttgggacgc cgttctacaa gactccgcgc
    13921 gaaaactgta cgcttaacag cgattatgac tgcctggact tgctaactct gcctgacgac
    13981 tatctgccgc gcactaacta cattccggca tgtgatgcac aggagtatgc aaaacgtact
    14041 ccatgcgtta catggactga actggctgaa gatgaaccga agaaggtaac agattattat
    14101 cgcttagcta tcagagccat gttggctcaa tcgggggaac gtacactaat tagtgctatt
    14161 tatccgccag aaataagtca catgaacgca gtacgttctt actgctatag ctcacagaat
    14221 ctgttactcg aacattcagg tatgtgtttt tctttacctt ttgattttat ttgtaaatct
    14281 actggcaagg caaacttaca tcagatgctt gatggtttct catacgtatt attcaatccg
    14341 agacaaaagg cattattata ctgcttagta ttatcattaa attctgtaaa tgatgtatat
    14401 gctggccttt ggcaatcctg ctacacccca gacttcaaca cccagcgttg gagccgcgat
    14461 ctcccgcagc tcccccagga tttcttcgcc aaactgaccc cagagtggca gcgtaactgc
    14521 gctttacgct ctgactacag tcgtcgtcag gcgctggtgg aaatcgacgt attggtggcg
    14581 caggcgctgg ggttaactct cgaagagctg cttaccattt atcgcgttca gttcccggtg
    14641 atgcgccagt acgaagcgga tacctggtac gatcaaaacg gtcgcattat ctttacccca
    14701 agcaaagggc tggtgggcgt tggcttgcct cgcaccgcgc gtaaagctga cctgaaaaac
    14761 ggctttgtct ttaacgtcga cagcccggag tggaccggcg gtgactgcac cgatcaagct
    14821 atcggttggg atgatgtcaa acatcttaaa accggtaccg tcagcgtcac ctttgatgat
    14881 tatacccgca gcgacgaagg tgagcgccgt accgtcacct ggcaggctcc gtttatcaag
    14941 ccagatcgcg aagatgacta caaagtggcc tgggcgttct ttgcacaaga taaggagagc
    15001 gcctgatgtt gccgtccgtt gtcagtcggc aggtcgccga cagcgttgcc gcctttttac
    15061 gggcggcgtt cccgctaaac agcccgctgt ttaacggtga gaataacgat aatgtctcaa
    15121 tgctggagca atttctggcc cagccggaaa cgctgctgaa aggaccatac ctttccgccc
    15181 agttgccgtt tcgtaaaagt gacttaccgc tgaatttttt ccctaatctg acgctgccgt
    15241 tcccgccgca cgcgcatcag gcccgggcat ttcagcgtct gggaagcgac gcgccacagc
    15301 cgacgctggt cgcaaccggt accggctccg gtaaaacaga atgttttatg ttcccgctgc
    15361 tcaaccactg cgcgggcgcg tcacaagccg gggttaaagc gattatcatc tacccgatga
    15421 acgccctggc aaccgatcag gccagccgtt tcgccaaaac catcgccagc gatccacaac
    15481 tgcatggcaa ggtgaccgtc ggcctgtttg tcggcgacag cgaaatcgaa cccagcaaaa
    15541 aaatgtcggc agaaaaggtc attacctgta agcacacgct gcgcgaaagc ccgccggaca
    15601 tcctgttgac caactacaaa atgctcgact acctgctgat gcgccctggc gatcagccgc
    15661 tctggcgtta caaccagccc ggttcattgc gttacctggt cgtggatgaa ttgcatacgt
    15721 ttgatggcgc gcaagggtcg gatctggcct gtctggtgcg acgtttgaag catcacatag
    15781 gtgtggataa ccaacgattc gcctgcgtcg ggacatcagc taccgtcggc gacgaactgg
    15841 ggcaactgct cgattacgca aaaacaatct ttgaccagcc gtttaccgat gacgccgtta
    15901 ttcgcgaaga tcgctacacg gcagcggagt acttgcagaa ttacgccatt gcgtacagtc
    15961 agtatccggg gccggaggtc gcttcggccc tggacccgca gagttacgct accccggtag
    16021 cgtatcttaa cgggcaaatc cctttgtggt tccccgacag cgacttgcaa ctcccggacg
    16081 atctggagag cgacgacggg cgtgaaaagc gtattgcgct ggggtcactg ttacgccgcc
    16141 acagcgtgat gcatgtctta ctccatgatc tgcaaggcgg gatcctctct gaacgagagt
    16201 gtctggaaaa tctccaggtc atgctggcag aaagcgccga ccatgcccga cgcgtgctgc
    16261 aaagcattct ggcactgatc gcgcaggcac gtctcgaagt accggaaacg gaacacgatc
    16321 ggcaaaaacg tctccaggcg aagaaagcgc gtccggtgct gccgtttgtg caactgcgct
    16381 cccagttgtg gcttcgtgag atgcgtcgtc tggtcgccag cgtggcgaaa acgccccgac
    16441 tggtgtttgc cgatgatgtg gcggtggaag atcgcgaaca ctatttgccg gttatccact
    16501 gccgtgactg ccacgcaact ggctgggcca gcctgcgtca tgggcaaagc gttcagttgg
    16561 aaacagacct cgacggcatt tatcgccagt tctttgaaaa agggcgctcc atcgtccttg
    16621 ctttcccgga taacaacgaa aaagcggtca gtggcgcaca ccagaaactg tgtcctacct
    16681 gtctgaaact gaaccgacag tccagcgtcc agtgtggaca ttgcggtcat acggaactgt
    16741 tgcaggtact gatcccggat atgctcaaag agcgtaagga cgagctggag tttgctaatg
    16801 aatgcccgta ctgcaacagc aaacagggga tttcgattct ggggtcacgg gctgccagtc
    16861 tgtcggcggt gatgattcac cagctttatg gcagccagtt caacgaacat aaaaagctga
    16921 ttacgttttc tgactcggta caagacgccg cgcatcgtgc cagcttcttt actgcccgta
    16981 cctggccgct gatgattcgt gggcaaatcg ccagtgttct tagcgaaaaa gagacattac
    17041 cgctggatgc gttcgcggcg cgtgtttgcc aacaaacgcg tgaacgttca ccggacgatg
    17101 ctcattttgc agcaaacgtc atcgcaccaa atatgcagtg gctgaatgat tatcagacgc
    17161 tgaaaaatga aggcaaactg gcggagaact ccgatctgcc ggagctggtt tccctgcgcc
    17221 tcgactggga ggtgtacagc gaatttaccc tgcgcgcgcg tatcggacgt acgctggaag
    17281 ccaccggtta tgccgttgcc ggagtggata aagatcgctt caatccggca attaccgccc
    17341 tgcatcaaca gctttgtgaa gagctgggtg atgagatgtc tggtgtgagc gtcgagcagg
    17401 tagcgcacct ggcgctgggg gttatctggt atcaacgcca ggccggggcc gttatgcatc
    17461 cggcgttcga aagttatcgc cgtgatggca cgacctttat gatgacgcaa aaagagcgta
    17521 cctcgcgcta tatgccttct atcggtccga aaacgcgtaa accggcttat gcgtcgtttg
    17581 aaaaaattaa tggtttccat cgcctgattg gcgcgaagtc taaccccagt tggtatcagc
    17641 attggatcaa tcgcacgctg agtaacggca ataatctgtt tatcagtagc gttgcggaaa
    17701 cagtgttacg acgcctgttt acggcgttga aaatagccgg agtagtaaaa gattttgaca
    17761 ccaaagggcg tgaagcctgg ggactggtgc cttcggcgct ggtggtgagc cgtgacgtcg
    17821 ttcagcttaa ttgctcatgt tgccgtgagg ttgtccgggc tccggccgat caaggctggc
    17881 agtggcgtaa tgcgccttgt ctctctttac gttgtgaagg tcgttaccag gaagtcgaca
    17941 atacggcgac ctggcgttgg gagaacatgg acatcgcgcg cgtacagggc gcagaacaca
    18001 cggggctgct tagccgtgaa gatcgcgaag tcaccgaaaa atcgttctac cgcggcaacc
    18061 aaccgtggaa tatcaacctg ttgtcggcaa caccgacgct ggagatgggt attgacgttg
    18121 gggatctttc caccgtttta ctctgttctg tcccacctgc tcaggccaac tacttgcagc
    18181 gtattggtcg cgccggtcgt aaagatggta acgcgttaaa cattaccgtg gcagaaggga
    18241 acccgcacga tcagtttttc tttgaagaac cactggagat gatgcagggc caggtgcagg
    18301 ctcctggcgt cttccttaat gcaacggcta ttctcgaacg ccagctggca gctttttgta
    18361 tggataactg ggtgaaaacc ggtgtaccag aatccgctat ctgtaaaaac gtcaaacaga
    18421 tgctggatga gctggagttc gggcgtaaat cgggttttcc gtataactta ctgcgatata
    18481 tcgaacagca tcatgcggag attgctgcgc aatttacggc aatcttcccc gatcttgccg
    18541 cagaaactcg ccaacagctg ttgtcttatc ttcagggcgc gccaggacaa cgctcgctgg
    18601 tgcagcgtat cgaagaagcg ttaaaactgc tggtggaaga tcgcaaatct ttgcgctcac
    18661 gcatcgataa actcaagcgc agtatcgata aactggagaa cgcgccgcgc gatcaaaact
    18721 ttgatagcga tatgcgcgag ttaacttcgg aacggcaggc gctgatggcg ctggtcaatc
    18781 aaatcaacaa taagcaaacg ctgaatttcc tcacggatga aggtctgctg cctaactacg
    18841 ccttcccgga agccggtatt acgttacgtt ctgtgctctg gcggcgtaaa gaaggcgggg
    18901 aagcacgtga atatcaaaac accactttcg aatatgaacg cccggcatca acggcgctgg
    18961 ctgagctggc tccgctgaat aatttctatg ccggtgggca taaagttgaa attgagcaga
    19021 tagacctgaa ggtctctgag ccggagaact ggcgtatctg tagccactgt aactacagcg
    19081 aaaatatcga ccagacgggc gaccagcata aatattgccc gaaatgtggc acaccggggt
    19141 gggccgatgc cggacaaaaa accacgctgc tgaaacttcg ccaggtttat gcccgcgcca
    19201 gcgcacgtga tagccagatc agcgacgaga gcgacagccg tgaacctgct ttcttccagc
    19261 gtcagctatt ggttagtttc gaaaaagaag atgtatcggc ggcatacgcg attgaggaag
    19321 gcgaagttcc gtttggtttt gaatttttaa gcaaagtcac cctgcgggat atcaacttcg
    19381 gcaaaatggc ggatgatgcc aatgagttga tgattgccgg agaagcgaag aagcgaaccg
    19441 ggtttaaagt ttgcctcggg tgcggcatgg tgcagcgtcc tcgcgatcat gaaccacgtc
    19501 atgatttgag ctgcaaatat cgcacggaac cggaaaaggc gaagttcgaa gattatctct
    19561 acctctatcg ccagctggag tcagaagcgt tgcgtattct gctcccggta agcagttaca
    19621 gtaatgaccg tgtcgtggag gcttctcttg gggcggcgat ccagctcggt cttaaacatt
    19681 acttcaaggg gaatgtcgat cacctgaaag gcgttgtgta ccgcgagccg gaaaatgaag
    19741 gcgaatcctg gcggcagtat ctggtgattt acgataccgt cccggggggg actggttctc
    19801 ttaaagaact gatgcgcact cccgacaatt tgctgaaatt acttgaactg gcctatcagg
    19861 caatagtgga atgcagttgc aacaatgata cgcataaaga tggttgttat cgttgcgttt
    19921 acgcttaccg tgaccgtggg cgcatgaagt atgtctcccg cgatcaggcg cgtctcttgc
    19981 tggcgaaaat tttgcaggca agagattcga tacgggtaat tgattccatc aaaaacattt
    20041 ccctcgaagc catgatgggc agtgagctgg aaaaacggtt tattagctgc ctgcaagaaa
    20101 ataaaaacct gattgtcagt cgcagttacg cgcatcaggg cgctgggtgg attattaaca
    20161 cccgcagcga accgatgatg agctggcatc ttaaagctca ggtggattta ggcgctaaag
    20221 aggcggtggg gatcccttgt cgcccggatt acgtgctata tccgcttatg cagtctgatg
    20281 ccattaaacc ggtggctatt ttccttgatg gctttgcgtt ccacaaaaat agcgtcgcag
    20341 aggatgtgca aaaacgccag gcgattcggg atagcggtaa tttctgggtg tggacagtca
    20401 cgtgggccga tctcaaagag ccagggctta aacatgttca ggatgtcctg ggacttgggc
    20461 ataacccgga tatgaaacag cctaagtttt ataacctgtt tcatgatacc aactttgcgg
    20521 cccttgaagc gtcgttccgg gaacgcaaca gttttgcact gctgctggat tatctcgccg
    20581 atccgggcgg aagaacgcag ttatggcaga gaatggctgc tgcacatgcc tgggtgtggt
    20641 tggatgtgaa gaagtcacag gacgcgggaa cgaaacaaaa gtatgcctat gaaatgcagg
    20701 aaaacgcgcc agcttggcgg ctagcagagc ttttgccaga cgaaccgttt gtctttggtg
    20761 gtttactcga tagctgtaac tcatcgcagc aatttattga gctggcttcg gtgttaccac
    20821 aacaagccat taaaccgacg accagcgtcg cacaaatgcg cagctggctg cgtttgcata
    20881 tctgttttga cgatcgctat acgcaggatg acggttatga agcgggactc aatggcttct
    20941 ggcgactggt aaacctgtta cagttcctgc cggatatgac ctttaccagc cgtaaagccg
    21001 tgcatttacc gcaggaaagc gttgacacta aagtttcggc aacgccagag gcagtaccag
    21061 agactgatga gagttgggct gagattattg agtttggctt attaagcgct gaagagatcg
    21121 cgttgcttca gtctcattct ctctctgcgc cgactctcgg ctacgaattg caaaatgatg
    21181 aaggtgaaat tattgcggaa gccgatctcg cctggccatc acaaaaatgg gcattgatta
    21241 ttgataatca agaatttatt ccattattca caaccagggg ttggcacgtt gcattcgggc
    21301 caattgacga aaatacattg caacatctgt ccggaggtga caaatgacgg tgcaaacgcc
    21361 caaggtggca ctttctgatg gttttctggg cgcattcgcg cggatcccaa aggcacagca
    21421 aaaaaaggtg caggaattca tctccaaatt ccgtcaggac ccgaccagta atggacttaa
    21481 ctatgaaaag atccacgatg cgcgcagtaa aaatgtccat tcggtgcgaa tcgatcaaac
    21541 ttatcgcggc attgttctga aacctgaaca gggtgccctg tatatgctga tgtgggtgga
    21601 taaacacgac gaagcatatg actgggcgcg gcggcatgat tgttcaattc acccggttac
    21661 cggcgcgatt caggtcattg atattagcta tatcaaaccg gcagcggaaa cagtggttga
    21721 taaaccgaaa ctcttcgctg cttacagcgc ggaacagatt ctggctctgg gtgtgccgcc
    21781 tgtctttatt gatcaggtga tggcgcttac cgatgaagcc gggcttaatc aacttgaaag
    21841 cataatgcca gcagaagcct gggaacctct gcactggctg gcagaagggc tggattacca
    21901 ggaagtgctg gaagagttta acgatcatcg tgatgagcct gttgatacca acgactttat
    21961 ggaggcgatt gaacgaagta aacgtcgctt ccatgtcgtt gaaaatgaac aggagctgtt
    22021 gcagatgctt aatgcgcctt tagaaaaatg gcgtgtattt ctgcatccga gccagcgaaa
    22081 attggtggag tcgcccgcta atggtccggt acgagtactg ggcggtgcag gtacagggaa
    22141 aacggttgtt gcgatgcacc gtgcgcggtg gttatctcag cgtctggcag ataaacccgg
    22201 taagaaagta cttttcacga cctttacgcg taacctggcg gcagatattc gcgctaatct
    22261 ccagcggttg tgtacgcgag aagagatggc gcgtattgac gtcgttaaca ttgatgcctg
    22321 gatgagcgac caactaaaac gccacggtta tgatttccgc gtggtatacg acagcgatga
    22381 agggcgacgc aaatgctgga actatgcctt gcagcaggca cctgccgaac tggggctacc
    22441 tgataatttc tatgctgaag agtggcaacg cgttgtccag ccaaatgcgg tttacacccg
    22501 ggaggaatac ctcaaagtca gccgggttgg acgtggtaca gcgttatccc gtattcaacg
    22561 ggcgaaaatc tggccagtct ttgaagaatt cagagcacaa atggcgcgtg ccaaactgcg
    22621 cgaaatgggc gatgccatgc atgaggctat tgttctgttt aaagaaaagc aggtccaact
    22681 accttatagc agtattgtgg tcgatgaagc tcaagatatc ggtgccccgg catttactct
    22741 tattcgcagc ctggttcccg agtcgcccaa cgatctgttt attgtcgggg atgggcatca
    22801 gcgtatttat cgcaataagg tcgttcttgg tcagtgtggt attaacgtcc gtggtcgtcg
    22861 cagtaaaaaa ttgaagatta actatcggac cacagaagag acgcgtcaat ttgctgttgg
    22921 gctgttaact ggtgtgaagg ttgataacct cgatggcgaa gcggatacca gtaatgatta
    22981 tttgtcatta ctgcacgggg aaaagcccat gattacgcat gcggcagatt tcaaagaaga
    23041 ggctgcaacg ctcgtcaagc aaattcaggc attgctggcg aatcaggttc gttctcagga
    23101 tatctgcatt actgcgcgta ctaaacatgc ttgtgaccgc tacgcatcag cactgaatga
    23161 cgccggaatt gagacattta atttaggcaa tgatagcggt gacagcgatg cacgccctgg
    23221 cgtgcgggtt gcaacgatgc accgtattaa agggcttgag ttccagtatg tgttcctggt
    23281 ggggattaac gaaggcgttg tgccggaaat aaaagcatta gcatccgatg atcccgttga
    23341 acagcgtgat gcattattta atgagcgcgc gttactgcat gtggctgcaa cccgtgccgt
    23401 aaaagggctg tttgtttcat catctggtgt ccctagtccg ctattagtgg ctgactgata
    23461 atatgtccaa aacaggccag gagccgaaac gctcctggtc tatataaccg ttctggcaac
    23521 tggcgatatt tatattgctt agctcccaac agccagacaa tatcaactta gaaattatat
    23581 tcgcttatgc ggtttgcata ttgcacagta caacctgttt tctctatatc taacgtaacg
    23641 tcaggatgtg gatgattttt ggatggggtg acacactgga caaatatagc tggcacgaaa
    23701 aggctggcat ttccagctga ccaatagtct ctggaaccat gatgatgata a
//
LOCUS       DAZFTN010000073        23588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFTN010000073 DAZFTN010000000
VERSION     DAZFTN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..911
                     /gene="hyxR"
                     /locus_tag="ACHKV2_003168"
                     /pseudo
     CDS             117..911
                     /gene="hyxR"
                     /locus_tag="ACHKV2_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            1065..1826
                     /gene="hyxA"
                     /locus_tag="ACHKV2_003169"
     CDS             1065..1826
                     /gene="hyxA"
                     /locus_tag="ACHKV2_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO6941008.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1868..2950
                     /locus_tag="ACHKV2_003170"
                     /pseudo
     CDS             1868..2950
                     /locus_tag="ACHKV2_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2940..3128
                     /locus_tag="ACHKV2_003171"
     CDS             2940..3128
                     /locus_tag="ACHKV2_003171"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6941009.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
     gene            3310..3978
                     /gene="ykgH"
                     /locus_tag="ACHKV2_003172"
                     /pseudo
     CDS             3310..3978
                     /gene="ykgH"
                     /locus_tag="ACHKV2_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(4221..4916)
                     /gene="ykgG"
                     /locus_tag="ACHKV2_003173"
     CDS             complement(4221..4916)
                     /gene="ykgG"
                     /locus_tag="ACHKV2_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6941010.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(4909..6336)
                     /gene="ykgF"
                     /locus_tag="ACHKV2_003174"
     CDS             complement(4909..6336)
                     /gene="ykgF"
                     /locus_tag="ACHKV2_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6941011.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(6347..7066)
                     /gene="ykgE"
                     /locus_tag="ACHKV2_003175"
     CDS             complement(6347..7066)
                     /gene="ykgE"
                     /locus_tag="ACHKV2_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6941012.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(7593..8447)
                     /gene="rclR"
                     /locus_tag="ACHKV2_003176"
     CDS             complement(7593..8447)
                     /gene="rclR"
                     /locus_tag="ACHKV2_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO6941013.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            8673..9998
                     /gene="rclA"
                     /locus_tag="ACHKV2_003177"
     CDS             8673..9998
                     /gene="rclA"
                     /locus_tag="ACHKV2_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO6941014.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            10107..10343
                     /gene="rclB"
                     /locus_tag="ACHKV2_003178"
     CDS             10107..10343
                     /gene="rclB"
                     /locus_tag="ACHKV2_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO6941015.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            10355..10948
                     /gene="rclC"
                     /locus_tag="ACHKV2_003179"
     CDS             10355..10948
                     /gene="rclC"
                     /locus_tag="ACHKV2_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO6941016.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(11108..11977)
                     /locus_tag="ACHKV2_003180"
     CDS             complement(11108..11977)
                     /locus_tag="ACHKV2_003180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6941017.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            12226..13083
                     /locus_tag="ACHKV2_003181"
     CDS             12226..13083
                     /locus_tag="ACHKV2_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6941018.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(13204..17457)
                     /gene="fdeC"
                     /locus_tag="ACHKV2_003182"
                     /pseudo
     CDS             complement(13204..17457)
                     /gene="fdeC"
                     /locus_tag="ACHKV2_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
     gene            18023..18583
                     /locus_tag="ACHKV2_003183"
                     /pseudo
     CDS             18023..18583
                     /locus_tag="ACHKV2_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            18791..19147
                     /locus_tag="ACHKV2_003184"
     CDS             18791..19147
                     /locus_tag="ACHKV2_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941019.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(19435..20361)
                     /locus_tag="ACHKV2_003185"
     CDS             complement(19435..20361)
                     /locus_tag="ACHKV2_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6941020.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAVSEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            20518..21438
                     /locus_tag="ACHKV2_003186"
     CDS             20518..21438
                     /locus_tag="ACHKV2_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6941021.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            21673..22815
                     /locus_tag="ACHKV2_003187"
     CDS             21673..22815
                     /locus_tag="ACHKV2_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO6941022.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            23289..23389
                     /gene="ykgR"
                     /locus_tag="ACHKV2_003188"
                     /pseudo
     CDS             23289..23389
                     /gene="ykgR"
                     /locus_tag="ACHKV2_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT         6466 a         5587 c         5158 g         6377 t
ORIGIN      
        1 aaatcaaaca aattagattt atattccaag aaaattaaat attcaggcaa ttaaacaaaa
       61 cctgacattt tgaatcaaaa tgtcaggttt tgtagctatg gcgtggacga gtctgaatga
      121 agaagcctct ggttattatc agtgcctgtc agtttaccag gctggcactg gagtctctca
      181 tcccagcgga caggtatatc gtgcgggtgt acagcaacgt cactacagag gttgaattcg
      241 ttttaaagac ctcctgtggc tatctgttgg ctgatatccc ttagcttcct tcgggggagt
      301 tagttcttct ggcttgtctt gtccgcttgc ggagtgatgc cgggcaatgg caggtctgcc
      361 tgaccggtga ctccgactgg ttcgataatg ctcctgctgc aagtctgtat aacggcttcc
      421 cacgcatcgg caccatgctg acagcgagtc gttatcagaa tcaaattatc cgctggttac
      481 tacgtccttt gcccggcgat atgagcgatt tctggctcct gactgcacgg gagttgagcg
      541 tgcttaagat tctgatgcag ggcatgagtt tcagtgagat tgcccgttat gaacaacgca
      601 gttcaaagac cctgcatgcg attgccacac gggcattaat gaaacttgga ctgggaactc
      661 tgagcgattt caggctgctc tatacgggat gcggtgacag cagagtgaaa cggaatatac
      721 ggctacatgc aagatatgtc ggacaacatt ccgctcatcg actcactcag tatgtgcaga
      781 acatggtatc cggaattctt tatccagaga aagttgccgt tggtgaatca ataaattctg
      841 acgccatttc cggaaaagta cttcccggac aaagaaaaaa tatttctaaa aaaagaccag
      901 gattgttatg acacaacagc gttgttacta ccccccactg cggcgcgtac ggactcgcta
      961 cagtgtaggg gccaaaatat ttctactgct ctgggctgca ggattgggag gcacgctgta
     1021 cagcctgtac agcgtgctgg cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt
     1081 tctgtccctc aggaattgta ccagcgcagt tcgcagctct atcaaccggt tctgtcggca
     1141 atgttacctg catcggaaca gaagaggagt tacgcaataa attgcggcac aggccacaga
     1201 gtgtcgtgat atcggcaggg cgacctgccg aatgcgctga aatgtggttc cgattttatc
     1261 gggatcactc attcgtggtg gtgttgtgcg tcgcgccatt ttttctgcca ccggatgtga
     1321 gtatttctgg cgttctcaaa aacctgaggt tactgaaacc aggaatgtca gtagagcatg
     1381 tcatcagtat tgcaaattcc tctggtgggt tatcagggct gaaacatgca gaaaatttgc
     1441 cagttatgga cagttactct gtctttatga aagaggtgaa taaccgcaca aagacaatcg
     1501 tcatgtcaga acgcttcccg gaaaagcaaa agaaggtgtt gtcgttgctt cttgccggac
     1561 acagctggga atacagtgcg cagtttctca aaacggggat aagacagata tggcttgccg
     1621 agcagtcgct aaaaaagcgt tggggaatac cggacagcat gtcattgcgg gaagccctgc
     1681 tgctgagcag caataaattt cacggtgatg gaaatgcatt ggaaaaaacg aatgccatga
     1741 cattgcgtga aaacggaaat accaacaggt attccgtggt ggtaaatgca gggacgcagg
     1801 ttttatctct gcataaatat aaataagtgg gaaattgaaa ttagttacca gaatggagaa
     1861 tttcttcatg aaaaacagta aggcatttta ccgcagcgca ttagcgacag ctattgttat
     1921 ggctctttct gcaccagcat tcgctgctga taacgcggta tcaactgatt cggttacgct
     1981 gaataaagat aagacgactc tggatcaaga tgttgttatt agcaatacag cagacaaaca
     2041 gattacagcc gtaacaatta atgcggcaga tgaagacctt aatgttgctt ttgctggtca
     2101 tgatattact gctgaatcaa cggcagacaa aaaattcgtt gaaggtgtaa aagttagtgg
     2161 taacaaaaat gttgtgatta atgctacaga ctccaccatc acagctcaag gtgaaggcac
     2221 ctatgtccgg actgcaatgg tcattgattc aactggcgat gttgttgtta atggcggtaa
     2281 tttcgttgca aaaaatgaaa aaggtagtgc gacagggata tctctggaag gggccaaggg
     2341 aaataatgtc acgcttaatg gcacaaccat aaatgctcaa ggtaataaga gttccagcaa
     2401 cgcctccacg gcaatttttg ctcaaaaggg tagcttattg aatagttttg aaggtgatgc
     2461 aaccgacaac attacccttg ctggctcaaa tattattaat ggtcggattg aaacaatagt
     2521 tactgccggg aataagacgg gaattcatac agtcaacctg aatattaagg atggctcagt
     2581 aattggggcg gctaataata aacaaacaat ttatgcctct gcttcggcac aaggcacagg
     2641 ttcagcaacg caaaatttaa atttgtctgt tgctgattca accatctact ctgatgtcct
     2701 ggccctttct gaaagcgaga attcagccgc taccacaaca aatgtaaaca tgaacgttgc
     2761 ccgctcttac tgggaaggta atgcttatac tttcaatagc ggcgataaag cgggtagtaa
     2821 tctggatata aatctttccg atagttcagt ctggaaaggc aaagtttcag gggcaggaaa
     2881 tgccagtgta tctctgcaaa acgagtctgt ctggaatgtt acgggatcct caactgttga
     2941 tgctctggct ccgtctgggt gatgagtctg tcaatgccag cattgacggt gcagcattcc
     3001 gcgtgggtgc aggtgtacag gctgatatca ccaaaaacat gggagcatat gcaagccttg
     3061 actacaccaa aggtgacgac attgagaatc cgctacaggg tgtagttggt atcaatgtga
     3121 cctggtaaga ataaagcagt cggttcccct gtcgactgtt ataccccctg caggcagcag
     3181 tatgctgcct gcaggctata aatatccagt attcatgatg ctggcttttt gctgatattt
     3241 aacccgaaca ttcctgatat agactattaa ttcatttatc tgcggcagat tttattttct
     3301 ggatatatta tgagtgaaca aatcaaacag gatatcgatc tgattgagat tttattttat
     3361 ctgaagaaaa agattcttgt tatccttttt attatagcta tctgtatggc aatggtgctt
     3421 ttatttctgt atatcaataa agataatata aaagtgagtt acagcctaaa aataaatcag
     3481 acaacacccg gcatacttgt taactgtgat agcaataata actttgcctg tcagacaaca
     3541 atgactgaag atgttattca gcgaattact acattttttc acaccagccc agatgtcaag
     3601 aacagagaaa taaagctgga atggtcagga gataagagag atttaccaac tgctgaagcg
     3661 gaaatatctc gcgtgcaggc gtctattatc aaatggtatg cgtcagaata ccataatggc
     3721 aggtaagttc tcgatgagat acaaacgcct tcagcaatta acagtgagct ttatacaaaa
     3781 atgatatacc tgaccaggaa ctggtcattg tatccgaacg gtgatggctg tgtaactata
     3841 agctcaccag aaataaaaaa taaataccct gctgccattt gcctggctct gggatttttt
     3901 ctaagcattg taatttctgt aatgttttgt cttgtcaaaa aaatggtaga tgaataccaa
     3961 caaaactctg ggcagtaata aacagcattc tgatagagag tgcctgtata aagctgaagt
     4021 gacaatgacc gggaagggaa cccgttttca ggaatggatg cctgtctttt tcactgtaaa
     4081 tatcctggca ttagtaatgc caggatattt acagaagagg agcataacca tgacggaacg
     4141 gggttaagtc aggttgtcct taacgctatt ttcaacctcg tatgccttct tcaggtttat
     4201 gtccagactt catacctctc tcagcaatcc tcaataatca gatacaccgc tttcaccgga
     4261 ccatgaaccc cgacgacttt gataagctca atgtccgccg ttgagctggg accactgatg
     4321 atgttaatac atgacggcat gcgttcaccg gcctgcgctt tctgatgcaa tctttctgcg
     4381 agttgcgcta cacgcggcag gatagtgctt ttacgcagga taaaaagaga tgattccggg
     4441 agcaggctca atgaacgccc acgctcggcg gcggaaaaaa gaaccacgcc tcccgattcg
     4501 gttaaaccat attcagcata cacgacaccc actttagcct gctctgcctg cgagatattc
     4561 ttggcacctt tcgccggatc ccaaacaacg gcattgcatt cctgctgcaa acgttcgcta
     4621 atccctaatt cctccagcct cgtgtcaccg ctaatcacga ccgactgatc tcccagctct
     4681 ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc tggtcagctc acagcgcgtc
     4741 aacataacat cgctggcaaa ctgaataaac gcgtcacagc gctgctgttg gttaagttgg
     4801 gtaagccgct cgttagcata gttattaagc ggcgcatctt ctgcttgcgg ttcaagtcgc
     4861 agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt cgcctcgatt atccattctt
     4921 tttctcctgc gcctgatgtt tcttaaacca actacggaaa ctctctccgt cagcttcagg
     4981 aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc
     5041 attgataaac cagcttgccg catgagcacc ggccatcatc ccgactttcc acaatcctgg
     5101 atgactattg gcataagcga acatttttat cgcccgttgc tctgcttttg cggtgatccc
     5161 tttttcagcc atcacccgac gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg
     5221 acacacgttg tcacaagctg tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt
     5281 atagccgcca agtagcggag aaatcaccgc accaattggc cctggataaa tagagccata
     5341 tccatgaccg ccaatatggc gatatgccgg acaagtattc atacaagccc cgcagcgaat
     5401 acagcgcagc acatcccgaa attcagaggc cagcacctca gaacgcccgt tatcgacaat
     5461 aaccagatga aactcttcag gaccatcaac gtgccccgct tcgcgaggtc ctgtcagcca
     5521 ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac
     5581 atctacctcg gcaaacgtgg gggcaatacg ctccattccc atcactgcaa tatgcgtttt
     5641 aggcagcgtg gtacacattc gcgcattacc ttcattggtc accaggcata ccgaacctgt
     5701 ctctgccacc gcgaaattac agcccgtaat acctatttca gcactgagga aatcttcgcg
     5761 gattttttgc cggatgaata aggtcatcgc ttcaggcgtt tccggcccct catagcccag
     5821 acgttcgtgt agcactcgac ggatctgatg gcgatcttta tgaattgccg ggaccacaac
     5881 atgagagggc ggatcttgat ccagctggag aatatattca cccaaatcgg tttcaatcac
     5941 ctgaatgcca gcatcctgca acacatgatt gacaccaatc tcttcggtca ccatcgattt
     6001 agatttcacc accttccggg catttttgcg ttgggcaacc tgtaaaatgt agcgggtagc
     6061 gtcttctttg gtttttgcaa aatagacgtg accgccgttt tgcgtcactt tttctgagag
     6121 ctggtacaga taagcgtcga gattactcag aacatgatca cgtatctggg cggcccgatc
     6181 gcgccactcc tcccagagcc ccaattcatc gaccattttt tgccgatttg ccccaatacg
     6241 ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct tcaatttgct gacggatgcg
     6301 tgtcttaaaa tctgtattac tggttttgat cgacatcttt atatcctcag cggctcatca
     6361 acacttcagc aatatgcatc actttgactt tctgcccttc ccgttgtaat cgcccactga
     6421 tgttcagcag gcaactcacg tcagcaccaa ttaaatactc agggcggact tccatcaggt
     6481 gcgcaacctt ttctttcacc atctcgccgg atatttcggc cattttgacc gagaacgtgc
     6541 cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa cagctccagt ccacgcacat
     6601 ttttcagcag cgtaagtggc tcgtccttca cccccagctt acgggccagg ctacaagatg
     6661 ggtgatacac cgctctccct tgcaaactgg cacctacatc gactacccct aatttattaa
     6721 caataaaaga ggtgagatcc tgcatacgcg cggcaacctt ttcggctcgt gatgcccatt
     6781 caggttcatc cgccagatac gtcgggtaac tttttacggc ataggtgcaa gagccagccg
     6841 gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat cagatttttc atccctggaa
     6901 tcgcttcttt gatataacca ctattgatcg caggctgacc gcagcatccc tgtttctccg
     6961 ggaaatttac gcgacagccg agtttttcca gtagcagcac ggagtctcgt gccattcttg
     7021 atttcagggc gtcaccaata caggtgacaa agaaattgac attcacaact aatactccat
     7081 tacttcatgc ccatttattc ccgatgtttc cccccacccg gggatcaatc atcaataaat
     7141 atggcgttct tttttaattg gctagcaaca taccacccct tatttgtatg acaaatgata
     7201 gtctttcatc caaacttcaa atgcaataac taattttatt tctttcggca tcgctcacat
     7261 tttatgggga aatcgacgat catttttggc ataagaaatc catttaaaaa tggctacatc
     7321 ccttatacct attcagagct acttattcag gggttattga gatatacact gatggtatac
     7381 aaaaaaatta ccgacaataa tcacgtatca agtaatagtc gggattttat ttaattgaag
     7441 caatttagtt tgcattttta tttaaaaact gagctagagt ccatcagaaa aaaatatata
     7501 aagcccaaac caccgttgga aatagaaata gttgaaaggc tcacaactcc cggaatgcgg
     7561 attatctttg ccctgatttc tggcggtttt attcagggtg caagctgtct gaccctttcc
     7621 cgatattctc ccggagtaca accaaactcg cggacaaacg ccttgtgaaa agatgattca
     7681 ctggcatagc ctactgactc agcgatcacc acaacaggga gcatttcccg ggaaaacatc
     7741 tgggccgcta tttgtagacg caactttgtt aatacagcca gcggcgtggt tccggaaaca
     7801 tcacggaaaa gctgggcaaa acttgcccgg gacatgtggg cgatgctggc cagcgattcg
     7861 acgctccagg cgtgtcctgg catttccagc atttgctgta ttaccgcacc aagacgtgga
     7921 tgcagaagca aactgagaat gtttttctca gtattaacct gtgcaatcca ctcacgcacc
     7981 gcgagggtaa agaatgtagc gcagatctgg ctacacagtg catccactcc cggcattgcc
     8041 gatctggatt cctgttgtaa aaacgggatc gcctccctca gccagttata ttccacgctg
     8101 tggttaaccg gtgctaaaaa cagcgtttcc ggcaaagacg ttaaaaaata acgtgccgaa
     8161 tgttgcagcc gaagagtacc acagacaata caggtcgatt cattatcgac atgactcagg
     8221 cgatgagctg aattttgtgg tagcaggacc acatttcccg ggcgtaatgt aaaaatctct
     8281 cccgtcggca tttccagctt cgccgctcct tgcgttaacg catgccaacg aataactgat
     8341 aattccccgg caccatgtgg aagctgccag tcacttccta acacgcaatt cttatcgatc
     8401 gttccttgtg gagcgttaag catcaacaaa cggctaaggg catccatatc agactcctga
     8461 gtggtttttg attacaaaca ccaacaatct tagtaaacaa ctaatctaat accatcaaat
     8521 agttaatcat gatttttgtt ttgtgattct gattaagaaa aagaaaatac cagacgataa
     8581 gacaaaatat caagcgttgt gaagaaatgt tatttgctct tttgcgtcta tagtcatgat
     8641 gtcaaatgaa cgcgtttcga caggaaatca tcatgaataa atatcaggca gtgattattg
     8701 gttttggcaa ggctggaaaa acattagccg tcacgctggc aaaagcaggt tggcgagtgg
     8761 ctctcatcga acaatcaaat gcaatgtatg gcgggacctg tattaatatc ggctgcatcc
     8821 caaccaaaac attggttcat gacgcacagc agcacacaga ttttgtccga gccatacagc
     8881 gtaaaaatga agtggttaat tttttacgta ataagaattt tcataatctt gcagatatgc
     8941 ccaatatcga cgtgatcaac ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc
     9001 atcggcctga gggaaatctg gaaattcatg gcgagaaaat ttttattaat accggtgcac
     9061 aaaccgtggt tccgccaatt cctggaatta ccaccacgcc aggagtatat gacagcaccg
     9121 gattacttaa tctaaaagaa ttgcctgggc atttaggtat tttgggcggc ggatatattg
     9181 gcgttgagtt cgcctctatg ttcgctaatt ttggcagcaa ggtaaccatt ttagaagcag
     9241 cttcgctgtt tttgcctcgg gaagaacggg atattgctga taatatcgcg acgattttac
     9301 gcgatcaggg cgtcgatatt atcctcaatg cccatgtgga gcgaatcagt caccatgaaa
     9361 atcaagtgca agtgcatagc gagcacgccc aactggcggt ggatgcactg ttaatagctt
     9421 ccggtcgtca accggctacc gcttcgttac atccagaaaa tgccggtatc gcagtaaacg
     9481 agcgcggggc aattgtcgtt gacaagcgat tacataccac cgcagacaat atttgggcga
     9541 tgggagatgt taccggcggg ctgcaattta cttacatatc actggatgat taccgcattg
     9601 tacgtgatga gttactgggt gaaggcaaac gtagtactga tgatcggaaa aatgtgcctt
     9661 attccgtatt tatgacaccg cccctgtcca gggttggtat gacagaagaa caagccagag
     9721 agagtggtgc tgatattcag gtggtgacat tgcctgtagc tgcaattccg cgcgccagag
     9781 tgatgaacga tacccgtggg gtattaaaag cgattgttga taataaaacc caacgtatat
     9841 taggggcatc actgctgtgt gttgactccc acgagatgat taatatagtg aaaatggtga
     9901 tggatgccgg gctgccttat agcatattac gcgatcagat atttactcat ccgtcgatga
     9961 gcgaatcact caatgatcta ttttcattag tcaaataaac tcaaaatcag acgccagaac
    10021 aaatattctg gcgtctcaga gaaaagaatc ttattaattc cctgttactc tataacttct
    10081 taatcacttc attgatggta ttttatatgt ttaaaaaatc cgttttattt gcaacactat
    10141 tatctggcgt tatggcattt tccaccaatg cagatgataa aataattctg aaacatatca
    10201 gcgtctcgtc agtatcagca tcaccgacag ttctggagga tgccattgct gatatagcca
    10261 gaaaatataa tgcttcatcc tggaaagtca catcgatgcg aattgataat aattcaaccg
    10321 caacagcagt attgtataaa taaggatatt cacaatggaa aaatacctgc acctgttaag
    10381 tcggggagat aaaattggcc tggcattgat tcgtctgagt attgcgattg tttttatgtg
    10441 gattgggtta ttaaagtttg tcccttacga ggcagacagc attacaccat tcgtcgcaaa
    10501 cagtccacta atgtcgttct tttatgaaca cccggaagac tataaacagt atctgactca
    10561 cgaaggcgaa tacaaaccag aagcaagggc atggcaatcg gccaataata cctatggttt
    10621 ttccaacggt cttggcgtcg tggaggtgat tattgctctg ctggttttgg ctaatcctgt
    10681 caatcgctgg ttaggtttat tgggagggct gatggcattt accacaccgc tggtaacact
    10741 ctcattttta atcaccaccc cggaggcatg ggtacccgca ttgggtgacg ctcatcatgg
    10801 tttcccttat ttatccggtg ctggtcgtct ggtattgaaa gatactctga tgctggcagg
    10861 tgctgtcatg ataatggcag attcggcgcg ggaaattctt aaacaaggca gtaatgaatc
    10921 cagttcaacg ttaaaaactg aatattgata atccgaacac tgtctgttgt tcactttttc
    10981 aggcgtcagg ccccctttct gatgatgaag cctgacgcca tattaccgga gatatgacag
    11041 agtgaagatc aatatcactc ccctgttaaa agattcttgt cgtctcaggt aaaatacatg
    11101 attaatatca atcatgtatt tttacagcaa gacaaccacg aacaaattca ggattaaaat
    11161 gttttaccga ctccccaaca taacccaaat caagcgaact aatttgtgcc atttctttct
    11221 ctgtcagtga gaaatcccag atagcaaaat tttcttcgat acgctgttga tgtgtcgatt
    11281 tcggaataac gaccactccc cgttgaatat tccaacgcag aatgacttga gaaatcgatt
    11341 tttgatgggc atctgcaata ctctgaagca gattattttc aaagggttta tgtcgtccac
    11401 cacctaacgg agcccatgct tcaggctgca cgttataata cttcatggtt gccagcgcct
    11461 ctggttgggc aaaataggga tgcaactcga cctggttgac catcggttta actcttaccg
    11521 tttcacaaaa gttcgccaga acatgaggat agaaattgga aaccccaatc gcttttaatt
    11581 tgccctcttc ataggcatct tcgagtgcac gccacgcact gaaataatcg cgcatagcct
    11641 gatgcaataa atagaggtcg aagtattcta gtcccgattt ttttaatgat gcttcaatac
    11701 ctgctgctgc cgtatcctga ttcaacatat cctgcaccca caatttagac gttataaata
    11761 actcttctcg ggtacataaa ccttcggata tagcttcacg aacggcttca ccgacggcat
    11821 cttcattacc gtataccgct gcagtgtcga tgagtcgata ccccgtgcgg atagcgttca
    11881 acactgactg tttgcacacg tttttatcgg taacctgaaa aacaccaaat cccatcatcg
    11941 gcattttcag attgttactt aatacggaaa attccactgt cagtccctcc attaggcatg
    12001 aacaatgagt ctacgttaaa acgtaacctc aaattagtat gtggattttg atatcactta
    12061 tgcaaaaaat tcattaataa tgtaggactg aaacctctct attttcgggg acaacgaagc
    12121 agacgctacc agtgcttttg ccttcgccct tgctgttttt gatacactta gggcccaggg
    12181 cataacgaaa atgtgcgata tgacaaagaa ttaacggaga atgtgatgat caggcagaag
    12241 attctacagc agctcctgga gtggattgag tgcaatcttg agcaccctat ttcaatcgaa
    12301 gatatcgcac agaaatctgg ctacagcaga cgcaacatcc agcttctgtt ccgaaatttc
    12361 atgcatgtgc ctttgggaga atacattcgc aaacgaaggc tttgtcgtgc cgccattctt
    12421 gtccggctca ccgcgaaatc tatgcttgat attgcaatct ctttgcattt tgattcacag
    12481 cagtcattca gccgcgaatt taaaaagtta ttcggctgct ctccccgtgt ataccgccac
    12541 cgtgattatt gggatctcgc aaatatcttc ccttcttttt taatacgtca acagcaaaag
    12601 acggagtgta gattagtcaa ctttcctgag acacctattt ttggcaattc atttaaatat
    12661 gatattgaag tgtcgaataa atcaccggat gaagaagtca aactgcgacg tcatcattta
    12721 gccagatgta tgaagaattt taagacggat atctatttcg tctccacgtt tgaaccgtca
    12781 acaaaatcgg tcgatttact cacagttgaa acttttgccg gtacggtatg caaacaaacc
    12841 gattcagaga tgccgaaaga gtggacaatt aaccgaggac tgtatgcctc tttccgctat
    12901 gaaggtgagt gggagcacta ccctgaatgg gcgcgaaacc tctatctgat ggaattaccc
    12961 gccagggggt tagccagagt gaacggcagt gatattgagc ggttttatta caatgaagat
    13021 ttcgtagaaa aggatagcaa tgatgttgtt tgcgaaatat ttatccctgt tcgtccggtt
    13081 tagctgtcgc tatatctaaa tgaacttata acgagactac tgtcaggcaa ttcaataaat
    13141 aatggcgttg aagatgtcaa cgccattatt gtttattaga atgttacttc catattctta
    13201 atattagttc atcgcctcct cgccctttgc cgtatcggca tccaccgcgg taatggtcac
    13261 cgtcactggc ttcatttcgt acccgccata cgtcagggcg ttaaatgtga acgtatagac
    13321 accaggcttg ctggtactaa atgtacgcgt gatttttccg ctcacgaaat ggtcattttt
    13381 cgacggcagg aactgataat ccttctccgt caccccttct ggcgtcttaa tgtcgaccca
    13441 catattcccc ccaacggggt tatttgccga catcagtgtc acctccagct tcgcagtcga
    13501 tatcccatcc gccaccagag aagtcacatc agatgatgcg gtgagtactg catttttatc
    13561 atccgcgacg aatttagatt cggcagtttt cgtcgattcc tgaccgtcgg cctgactcac
    13621 tttcgccgtg agtgtatatt tcgctgcgac agtggtcgtg gcggtgaaaa tagcctgtcc
    13681 ttgctcatta gttttcgccg tcccattggg agtcagaact aaatttgccg ggctggcagt
    13741 cagcgtcact tcgctatctt tcaacaggtt gttattggca tcagtcaccg taactttata
    13801 cgtctgtttg gcattgccgt ccgccagtgc atagttgcca atcacctcaa taccggagac
    13861 ggttgcggta gccttatcgg ctacaaacgt tatagtctgc gtattcgaag gctggctatt
    13921 ccctgccgat gccgtgatct catacgttcc ggcagtcact gaaaccacct gcaactctgc
    13981 tttcccctca tcgttggctt tcacccagac attatcgcct gtaagcggct tgacaccccg
    14041 gggcagatta aaggtcacca ggctccctgg aacagggtta ccatttttat cctcaacgtt
    14101 cgccgtcagc gtaaaggcat ctttgccgtt tgccactttt tgagcagcgg cgtctacctg
    14161 caggtttgcc tgaccggtgc tggcatccgc gttgaatttc accgtgaccg tcttctgagc
    14221 accattcacc gaagcggaaa tattgtgttc tcccgcaacc gttgacataa gctcaatgtc
    14281 cgctttacct gccgcattag ttgttactgt attccccgtc ttgctggtca caccctgcgg
    14341 taaagtcagc gtaacttcct gcccctgcaa cgggttacca taggtgtcca caacggtcag
    14401 ggttatctgg ttagcagact gtccattagc cagttggtta ttaaccttca ctgtcatatc
    14461 acgaatctca gccttagatg cgtcacctgc aacgttcagc accagtggct gagcaacggc
    14521 attttggccg ttcactcgcg gcatcacaga caactgaccc gcggcagatc ccagcgttaa
    14581 ggtcgacacg tagaccccat tacctttctc tgtccagttt cctgctgaag gacgctcact
    14641 ccccgtgctg gcggcaccgc taaacactgg tgcatctggc ttcagcccgg tgaccgggtt
    14701 cccgtacgca tccttcacgg tgaaggtgag ttccgtcgtc gttttgacgg tcggagcctt
    14761 attgtccgca acaagcgtag agttcgctga tgacatctct ccggcgatga ccgtcaactg
    14821 cgcggcttcg gtggctgctg gctggccgtt gaagagaggc atgacgcgaa gctcgcccgt
    14881 ctttccacct gtagtcaacg tagcaacata ggaaccgtta cctttttcgg tccaactgga
    14941 aacggtcgcc ccttcagagg cggtccccgt caaacttgcc gacaacgcaa gaccactgat
    15001 agcgttgcca tgcgcatctt tcgccaccag cgtcacggtt gtgctttcgc cggcttttac
    15061 gtgatcttct gcgacagaga ctttcgactg gtttgaagat aacgcatcag ccaccacggt
    15121 tacttttgcc gcatttgctg ccgcattctg tccatttagc ttcggcataa cttctaattc
    15181 acccgccgta gagccgagag taatctgcgc agtccacgtc ccatcaccat tatttgtcca
    15241 gcccgatgcc gtagaacctt cagcagcggc acccgctaat gacggcgctt ccggcgtgag
    15301 gctggtcaca gggttgtcat aggcatcttt taccgtccag attgccgtaa ctgtcccccc
    15361 aaccaccggt ttatcaggat tcagggtgat ggacgaatgt gctgcatcaa gcggcccggc
    15421 aacaaacttc agcgtttgtt gtaatgtgcc cagctgatac tgctcgctga aagcacgcac
    15481 aacaacgttt ccggcacggg tcgaagaaac cgcggcgctg taaacgcctg gttttatttc
    15541 cgaaatggca ccaacggtta caccattagt gtcttgcgga acaaatcgca agcggctggc
    15601 ttctcacgtc accggattac cctcggagtc caccgcagtc aacgtcaacg tataggcttg
    15661 ctgaccatca gcaaccacgt cacctgacgg ctcgttagcg ctcagggtgg agtttgccac
    15721 atccatcatc gtggcccgca gttctgcagt gacggttttg ctcatgccat caacgctaac
    15781 agtaatcgtt gcctcacctg actgcgttcc ggtagtaaag acagactgat acacccctgc
    15841 ttcagtttcg gtgaactcac ccagtgttgg ctttgcctgt gatttagtgg ccttcaggga
    15901 acgagtcaca atatttccag ccggtttgaa agttagttca gtcttgatct gatctttcat
    15961 gcccgtgact ggctggccct cggcgtcgcg cagagacagc accagcggtt tttgctcatt
    16021 accgttagca agcatttgaa tacggctctg accgtcaagc gttaacgccg tgcgatcggc
    16081 gctcatacca gctccggtaa tgaccacctc tgtctgcacg cgttttgagg tattgccttt
    16141 gttatcgtag gcaactgctg aaatcgcata ataattgtct ttgcctggac gataagccgg
    16201 gagcgttact tgccactgac taccctgacc ggtaattttg ccaccttcag ccagtaatga
    16261 cggcgcttcc cactgcacat ttttcagtcc gtgagttgct ttgctgacca caagccccag
    16321 ggaaagtgtc tgaccgccct taccttcaat acgctcaggc agagcaatac ggatcacttc
    16381 agatttgcgg tactcaagaa cgatgttgtt attacgctca accaggtcat agcggctgcc
    16441 tgccagtacc cgacgctcgc gaatgctatc cgtatcgagt tgtttcgcca aaggttcgcc
    16501 aattcggtag ttaacttcca ggccaaagcg agtgtcattc tcaccgctct tgccctgctt
    16561 atgcccggcg ctcagtgtca gaagaggcac tggcgtatag gtcacctcgg cagaaatagc
    16621 atgcgggtct ttctggcgct tatctttacc aaacagcccg acttcatcgc cataatactg
    16681 ttcatacatc aggcttgcgc caagctgcgg ccaggcaggt aaatagccct ctgcgcggat
    16741 atcccaacca ttcgccgggc gttcctgata atcctcaata tccggcgatt ttttccagcc
    16801 agaagcccga atataaccat tggcgctcag tttcagataa tcgcgccagt attccgcacc
    16861 aacaccaatg cgggtatgac tacgggataa atcatggtcg ataaaggtgt tcacccccgc
    16921 catccagtca tttcctgaaa aatgacgcca gccaaaacca atatttgact gagtacgatc
    16981 gtctgtacga tgtattgccc cctgagtgaa caacatattt gtcggcgtat cataaatcgg
    17041 ataaagcatt tccagcgaag aatccttcag cgagaaatct ttatcgacat tcagtttgac
    17101 gcgcgctgta ccatatttcc cgagccactc ctgtatttcc tggttagctt tagcggtggc
    17161 cattccggta ataaaattac gtgtcgcatc gctatctggc tgactgctta aaaatgtccc
    17221 ggcatttgcg gcaaacgacg cgacattttt ctccacgtta ttatcagcag ttaccgtagt
    17281 atttcccatg ctcaaccgtg gctgaaccgc atgctgcgca cgtgccgcca ttactggggt
    17341 aaaggtgaca gcgagtggaa aaagaacctg aacagagata tttgcccacg ccacgcagcg
    17401 ggccagaact gaataacgaa atcgtggttg tttatgacct gttttataac gtgacattga
    17461 gataatttcc tgatgaacga attaacgaac ataggcagac acaaataatg gcgtctgctt
    17521 gtcagaaaaa ccggggattt cccggtttcc ttttccgcag aaaaggaaat acctttatgt
    17581 aagaaagtac accgccacct aatatatcca gtgcctgtag ccactcatca ggtggttggg
    17641 cgcgggatta tatttttatt tatgggtgaa aatttagctt tttgtgcaag tcattaaata
    17701 gatacttaag ttatatttct tactctgcgg acagtgaata attcattatt cagagtgaat
    17761 aataatgacc tgcctttaat tagcaactat tggaaatacg tcgaagtagg tatatacgcc
    17821 aggataaacc gatatatttt ttatggatat tcattttcct tcatcggact gtaaattgcg
    17881 gagatatcga acttgcacta cccatcgcaa cactcaaaca gatttatgaa tttcgttcat
    17941 aacccctgac aaatcctgcc gtctaatcga gctcttctct ttacgttatg cttctcatga
    18001 acgataacgg aaggataaca ccatgcaaaa agtaaaactg aataacggta ttgaaatgcc
    18061 cctgctgggc tttggtgtat ttcagatgac cgatgccgcc gaatgcgaaa gagcggtcat
    18121 tgacgctatt aacaccggtt atcgactgat tgacaccgcc gcctcctatc aaaatgaacc
    18181 ccaggtcggg aacgcactga aacaaagcgg tatcgcccgt aacgaactct ttgtcaccac
    18241 gaaactctgg ttgcaggata ccagttacga aggggccaaa gcccagttcg aacgctctct
    18301 gaatcgactg caacttgatt acgttgacct gtatttgatt catcagcctt acggtgatgt
    18361 ccacggtgcc tggcgtgcca tggaagagct gcaacaggcc ggtaaaattc gcgctattgg
    18421 cgtcagtaac ttccatcctg accgactggc tgaccttatc gcctttaata acgtcgttcc
    18481 agcggtaaac caggttgaag ttaacccttt caatcagcag ctacacgccg ttccgtggat
    18541 gcaaagccgt ggtattcagc cagaagcctg ggcaccgttt gcagttacag ggcggtaagc
    18601 gattatgcat ctacctattt acgtcggtca ctcagtcaga gtcttgccca tattcaatga
    18661 ggttcaacac cgcagccaat gaaaagtagg ccatgcgggg attaatagca ttactcatta
    18721 accaatcgct cttttcattt acgtgctttt tatgtaacat attgctttta ttgatcttcc
    18781 tggagtttgc atgaataaga atctcatcct cgcatttgca ttattttcct tacctgtatt
    18841 tgcagaagaa gatctggggc caggcaaata tgtttgtgac attaggattt ccagcctgga
    18901 taccgctact caaattctgt caaaatccgc aacagttctc gataatggaa acaatttcat
    18961 cgttcaaatg ccaaatggag atcaattgta ctctccagat ctggaaaatg ttgatgatgg
    19021 tataaaacaa aaagcaacaa taggtggagt gacatttatt cgtcgtccaa cttttaacga
    19081 tcgcttcatc gtggaagatg gaaatacagg attcttctat aagatgcgaa actgcgagaa
    19141 gaaataaagg aagtttgata gcgatatcct cctgatttca ggctttgtac ctgttcaaga
    19201 cttacaacta caagccgcta cgtgttaacg cctcccaaaa taaccaccgt taaccgtagc
    19261 ggtcatagat actattattt taaccacatt actggtgata atgataattg atattatggc
    19321 cgtatagaaa ataagtatta attatcagaa actttattat gtaaacgcat cctaatgatt
    19381 acgttattcc tcttaccgat attcgcccag agcaaccatc aattacacat atcatcaatt
    19441 ttcgagtttt actcttttct ccaccataaa atcaataaaa gcacgaactt tagagggcat
    19501 tctcacccgt gatgaaacat aaatataaaa cccaggaaat gaaacagccc aatcctcaag
    19561 aatcggctgt aacttgcccg cattgatatg ttcagcgatt gcaaaatcca gatgttgaat
    19621 gatacccgtt ccattcagcg ccattcgtat catgctttcg tcatcattag aaatatactt
    19681 acctgaaacc tctatttcga actcttcatt accccccaca accttagaaa actcccatgc
    19741 agagagtgca ccgctacttt tatgccgcaa ccgaaggcaa ttatggttac tcagttcctg
    19801 aggcgtttca ggaataccgt gttcttttaa ataatctggc gatgccacag taaccagttt
    19861 aataagcgga gaaacaggaa cggcagtcat atgctcatcc agtccttgct caagacgtat
    19921 gcccacatca cagccgtccg caataatgtt agataaaccg tcatccataa tgagttctat
    19981 ctttgttttg gggtaacgag taagaaactc gccaatatga ggctcaacaa gcatccgtgc
    20041 tgcaacacgg gaggaattta ttctcaacag gcctgtaggt tcaagttggg tatcaccaac
    20101 atctttcaaa gcatcatcaa tttgccccaa agccgacacc agcacttcat acagacgctg
    20161 tccttcctca gtaagcgaga tattgcgggt cgtacggtat atcagcttgg tgttaagacg
    20221 ttcctcaaga gattttaaat tctgggacaa tgcggccagc gtaataccca tttcagaaac
    20281 ggctctggaa aaactgccgg cacgaacaat cagcgcaaac caggcaagag aaggcaaaat
    20341 tgaaggctct attttcatca ttgtaaacct taacttaaca ctgtgataga tattagacca
    20401 ttgatgataa ccaatacaag tcatactatt acgccgttct cgatatgaac acaaatgcca
    20461 gttgcggata atagaaatga tatttaaatc tgtttaggca aatccccgga gcttgaaatg
    20521 aaaactgttt cgataaaaca cacttactgg gatatagctg ctgatatcta tttccctgac
    20581 gattttaata gtgaaaaaaa gtatcctgcc attatcagcg cccatccaat tggaagctgc
    20641 aaagaacaga catcgggtag tgtgtatggt gccgccctgg caaaagctgg ctttatcgtc
    20701 attgcttttg atgctagttt tcagggaagc agcggtgggg aaccgcgtta tctggaagat
    20761 ccaacaatgc gagtaaaaga cttcagcatt gtcgttgatt accttaccag cttaccttac
    20821 gttgatgccg ggcgtattgg tgtactgggt atttgtggtg gaggtggata tgccattaat
    20881 gccgcaatga cagaacgccg aattaaagct attggtactg tcacgggtgc aaactacggg
    20941 cgtcttatgc gtgagggatt tacagcgttt aatcctatcg gagcacttga agcaatggcg
    21001 caacaaagaa cagacgaagc taacggcgca aaacttcgcg tagatgattt actcccctct
    21061 tctccggatg cagctcttga agcgggacta acagaaatag acctttttga agccacagaa
    21121 tattatcgtt cgccacgagg ctatgcacct aatggggtaa atcgttcgct attctcacat
    21181 cagactgttg ccgtgggttg ggatgcattt catcttgcag aagtgctgtt aactcaacca
    21241 ataatggttg tggtgggcga tagagtgggc gcatttggtg cctaccgtga cggctgcgaa
    21301 attattggcc gcgctgcttc aaaacataaa gagctggtcg tggttgaagg ttattcacat
    21361 tacgacctgt atgataagcc agaacctgtg aaacaggcac tcgataaatt aattcctttt
    21421 tataaaacac atttgtaata attttacagg gagatccctt atctccctga catttatcct
    21481 ttattgacta cacaacaata ataaaattat tcaaaaagaa atttcattaa aaaacaaaaa
    21541 agcagcaact caattattag aaacccttta aagggattat aaataaagcc agaactcttg
    21601 gttccaaagc tggcaacaac taaccagtta aaacatacgc atcaaaatca gataaacatt
    21661 tgaggtattg ttatgacaaa taaacaccca tcattgtttt ctccttttat gctgacagaa
    21721 aaaataaagc tacgtaatcg tatcgttatg gctcctatga cgacctggtc agcgaatcct
    21781 gatggaacca tctctgagca agaactcgaa ttttataaac gtcgctcaca aaatgttggt
    21841 ttagtaataa caggatgtac ctatgtcacg ccaagcggca ttgggtttac tcatgaattt
    21901 gccgcgtatg acgaccgttt cattaacagc cttgaaaagt tagcagcggc agccaaaagt
    21961 ggcggtgctc cggctatttt gcagattttc cacgccggta ataaagccat cccggagctt
    22021 gtaccaaata acgatgttat tagtgccagc gcatcaagcg taaaatctgg tgactttatg
    22081 aagagagtgg tacaaagcag ggaaatgact gaaaacgaaa ttcaggaaac cattagagca
    22141 ttcggagatg tcacaaaaag ggcgattaaa gcaggttttg atggcattga acttcatggt
    22201 gcgcacggat tccttctaca gaattttttc tcccctctat ttaaccagcg taatgaccga
    22261 tggggaggag atctcgaagg acgtatgcgc ttcccgctgg ccgtattaca ggaagtaaaa
    22321 aatgtagtct atgaatatgc gacaaagccc tttgccatcg gttacagaat atctcctgaa
    22381 gaatctgcaa caggggggct gcggatagag gatacttata aactccttga tcggctcatt
    22441 tcatccggta tttcgtatat acatacctcg ctggtcagta ttaacgatag ctaccctgtt
    22501 gagtcgccaa atggcccccg aactattgaa ttgattttaa atcatattgc aggcagagtg
    22561 ccggtaatcg ctgcaggtaa aatacgcaca ccgtctcagg ctcaagaagc aatttccact
    22621 ggcctgccac ttgttgcgat cggtaaaggg ctggtgataa atccagaatg ggtgacttta
    22681 gctgaatcag gtagaggcca tgaaattcaa acaaccctta atccacaact tgttcctgaa
    22741 ttaaccattc cggataaact atgggatcaa attcaggcat cgaaaggaac tggctggttt
    22801 ccgttaatgg attaaatatc tattactctc ccgagagtaa aactatcaaa tcaatgagta
    22861 accgatacag cctcacaaat tctttcagat aattaacccg tcactgcggc acgttctctc
    22921 ataaggcgtg cctgcagtgt aatctagtat gtatccggca tagttgacgg tttaatagag
    22981 ctcaattcct cgcccaggtt accaacaacg atatcttgta taacacccca ggagatacga
    23041 cattatgatg agatgcgata tgctcctcag cgccaatcaa cgccaggact gggccagtct
    23101 tgagatcaga ctcattcccg gaactgactt caggcgacat atatcaatga cgttggggca
    23161 ataaaagatg cctgatgtaa tacatctatt caaatttgtg atacatgtca aaaaattggt
    23221 tgaccaaact cgttatttat ataagggcac ttacgaagtg cgctcttttt taaagcgagg
    23281 aagtaccaat gaaagagaat aaagtacaac aaatcagtca taaactgatt aatatcgttg
    23341 ttttgtcgca attgtagaat acgcctattt atttctccat ttctattaat aacggcaata
    23401 aactgttcac ttcagtgata tttaaaatat gcatcctctc cctttttgta agtaattatt
    23461 atatccgtgg gagaggaata cacgctgtca ggtaatcaat catactgcga taaatcatcg
    23521 gccagtaaag tggagataac ctccattctc gaaaaatcca tactctcagc gaaactatca
    23581 tcaatcac
//
LOCUS       DAZFTN010000074        22820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFTN010000074 DAZFTN010000000
VERSION     DAZFTN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..638
                     /gene="ydcL"
                     /locus_tag="ACHKV2_003189"
     CDS             <1..638
                     /gene="ydcL"
                     /locus_tag="ACHKV2_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO6941023.1"
                     /translation="LCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATGKPVLRW
                     VDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPLVT
                     TAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELI
                     DAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
     gene            complement(673..1884)
                     /gene="ydcO"
                     /locus_tag="ACHKV2_003190"
     CDS             complement(673..1884)
                     /gene="ydcO"
                     /locus_tag="ACHKV2_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO6941024.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            1937..2473
                     /gene="sutR"
                     /locus_tag="ACHKV2_003191"
     CDS             1937..2473
                     /gene="sutR"
                     /locus_tag="ACHKV2_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO6941025.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2546..4507
                     /gene="rlhA"
                     /locus_tag="ACHKV2_003192"
     CDS             2546..4507
                     /gene="rlhA"
                     /locus_tag="ACHKV2_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO6941026.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4599..4829)
                     /gene="yncJ"
                     /locus_tag="ACHKV2_003193"
     CDS             complement(4599..4829)
                     /gene="yncJ"
                     /locus_tag="ACHKV2_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO6941027.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            5051..5227
                     /gene="hicA"
                     /locus_tag="ACHKV2_003194"
     CDS             5051..5227
                     /gene="hicA"
                     /locus_tag="ACHKV2_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO6941028.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5273..5689
                     /gene="hicB"
                     /locus_tag="ACHKV2_003195"
     CDS             5273..5689
                     /gene="hicB"
                     /locus_tag="ACHKV2_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO6941029.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5768..7174
                     /gene="ydcR"
                     /locus_tag="ACHKV2_003196"
     CDS             5768..7174
                     /gene="ydcR"
                     /locus_tag="ACHKV2_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6941030.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            7419..8564
                     /gene="ydcS"
                     /locus_tag="ACHKV2_003197"
     CDS             7419..8564
                     /gene="ydcS"
                     /locus_tag="ACHKV2_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO6941031.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8582..9595
                     /gene="ydcT"
                     /locus_tag="ACHKV2_003198"
     CDS             8582..9595
                     /gene="ydcT"
                     /locus_tag="ACHKV2_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6941032.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9596..10537
                     /gene="ydcU"
                     /locus_tag="ACHKV2_003199"
     CDS             9596..10537
                     /gene="ydcU"
                     /locus_tag="ACHKV2_003199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6941033.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10527..11321
                     /gene="ydcV"
                     /locus_tag="ACHKV2_003200"
     CDS             10527..11321
                     /gene="ydcV"
                     /locus_tag="ACHKV2_003200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6941034.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11343..12767
                     /gene="patD"
                     /locus_tag="ACHKV2_003201"
     CDS             11343..12767
                     /gene="patD"
                     /locus_tag="ACHKV2_003201"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO6941035.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12864..12959)
                     /gene="yncL"
                     /locus_tag="ACHKV2_003202"
     CDS             complement(12864..12959)
                     /gene="yncL"
                     /locus_tag="ACHKV2_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO6941036.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13154..13327
                     /gene="ortT"
                     /locus_tag="ACHKV2_003203"
     CDS             13154..13327
                     /gene="ortT"
                     /locus_tag="ACHKV2_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO6941037.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13413..13646
                     /gene="ydcY"
                     /locus_tag="ACHKV2_003204"
     CDS             13413..13646
                     /gene="ydcY"
                     /locus_tag="ACHKV2_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO6941038.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13647..14096)
                     /gene="ydcZ"
                     /locus_tag="ACHKV2_003205"
     CDS             complement(13647..14096)
                     /gene="ydcZ"
                     /locus_tag="ACHKV2_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6941039.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(14093..14611)
                     /gene="mddA"
                     /locus_tag="ACHKV2_003206"
     CDS             complement(14093..14611)
                     /gene="mddA"
                     /locus_tag="ACHKV2_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO6941040.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14792..15829
                     /gene="curA"
                     /locus_tag="ACHKV2_003207"
     CDS             14792..15829
                     /gene="curA"
                     /locus_tag="ACHKV2_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO6941041.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            16027..16692
                     /gene="mcbR"
                     /locus_tag="ACHKV2_003208"
     CDS             16027..16692
                     /gene="mcbR"
                     /locus_tag="ACHKV2_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO6941042.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16728..18830)
                     /gene="pqqU"
                     /locus_tag="ACHKV2_003209"
     CDS             complement(16728..18830)
                     /gene="pqqU"
                     /locus_tag="ACHKV2_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO6941043.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            19072..20133
                     /gene="yncE"
                     /locus_tag="ACHKV2_003210"
     CDS             19072..20133
                     /gene="yncE"
                     /locus_tag="ACHKV2_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO6941044.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20248..21747)
                     /gene="ansP"
                     /locus_tag="ACHKV2_003211"
     CDS             complement(20248..21747)
                     /gene="ansP"
                     /locus_tag="ACHKV2_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO6941045.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            22014..22631
                     /gene="yncG"
                     /locus_tag="ACHKV2_003212"
     CDS             22014..22631
                     /gene="yncG"
                     /locus_tag="ACHKV2_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO6941046.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            22707..>22820
                     /locus_tag="ACHKV2_003213"
     CDS             22707..>22820
                     /locus_tag="ACHKV2_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO6941047.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQ"
BASE COUNT         5821 a         5591 c         5849 g         5559 t
ORIGIN      
        1 ctttatgcgg cttgttggct ctgtctggtt gtgcctctaa aatcacccag ccagataaat
       61 attctggttt tttaaacaat tactctgatt taaaagaaac aacctcggct acaggtaaac
      121 cggttttacg ttgggtagac ccgagttttg atcaaagcaa atatgacagc atcgtctgga
      181 acccaatcac ttattatccg gtaccgaaac cttcgaccca ggtagggcag aaagttctgg
      241 ataaaatttt gaactatacc aacaccgaaa tgaaagaagc gatagcgcag cgtaaaccac
      301 tggttaccac cgcggggccg cgtagtctga ttttccgtgg ggccattacc ggtgtagata
      361 ccagcaaaga agggctgcaa ttctatgaag tggttcctgt tgcattagtg gttgcgggga
      421 cgcaaatggc tacaggccac cgtaccatgg atactcgctt gtattttgaa ggtgagctga
      481 ttgatgcagc gactaataaa ccggttatca aagtcgttcg tcagggcgaa ggtaaagacc
      541 tgaataacga aagtacgcca atggctttcg aaaatattaa acaagttatt gatgacatgg
      601 cgaccgatgc caccatgttt gacgttaaca aaaagtagtt ccagacgcgc catcgttaga
      661 tggcgctttt tattatcggt ttctgtcagc gattaaattc aacaccacgt aacaaacgcc
      721 tccggcaatt aatccccaaa acgcagaacc aatcccgacc agcgtcaatc cacttgccgt
      781 taccagaaat gccaccaccg ccgcgtcgcg ctcacgctca ttatgcagcg cctgatacaa
      841 actgccgcca atggtactca acagcgccag acctgccagc atctggatcc aacttacggg
      901 cagggcagcc atcatcccgg taatggcact accaaacaga cctgcgagca aatagaaaat
      961 gcctgcaacg gcagctgcca gccaacgttg atctttatcc ggatgcgctt ccgggctttg
     1021 gcaaatagcc gcggtgattg ccgcaatacc gacggaataa acgccgaaag gggaaaaaac
     1081 cagtgccagc aatccagtaa acacaatcaa cggcgaaaca ggagccgaat acccggctgc
     1141 tttcattgct gcgatacccg gtgcgttttg cgatgccatc gtcaccagaa aaagggggag
     1201 tgcaacgctc aggctgtgag caaacgaaaa atcaggggta atataagtgg ggagaacggg
     1261 tttaaagaca acatcagttg tgacaacgtc accttgcgcg ataacgatca cgatcccaat
     1321 aatcatcgcg gcaattaccg catagcgcgg cgcaacggcc ttggttgcca gccataccag
     1381 caacatactt ccacacaacg taaattgacc gtccagactg gcaaacgcct gtaaaccaaa
     1441 gcgtaataaa atcccggcaa gcattgctgc cgcaagcgag tgcggaataa tgcgcatcag
     1501 acgagcaaag agtcccgtta tgccgcagag gactattaac gcgttggtga caataaaaac
     1561 gccgatggct tcgttaagtg ttagtccctg caatccggtg accaacaaag ccgcgccagg
     1621 cgttgaccat gcggtgagaa caggtacgcg ataccataat gtcagagtca gcgtactgac
     1681 gcccattgcc agccccagcg ccgtcatcca gccagagatt tgtgcagtgg tggctccggc
     1741 gacaatcgct gcttgccaga ttattgccgc tgaactggcg tagccaatta ataccgccag
     1801 aaaccccgcc agtagcgtgg gtggaggaac agaaaacaga cgcatagtca cctcgtgcgt
     1861 tatagcgtac aggcaccgta gcatttgtcc gttataacgc acaagtgata aacttccgtt
     1921 ttgccggagg agtcgcatgg aaaatctcgc tcgcttttta tccaccacac ttaaacaact
     1981 acgtcagcag cgcggctgga gtctttcgcg actggcagaa gcgacgggcg tttctaaagc
     2041 aatgttagga cagattgagc gtaatgagtc cagcccgacg gtcgcgacat tatggaaaat
     2101 tgccactggg ttgaacgtgc cattttcgac atttatttct ccgccacagt ccgctacgcc
     2161 ttcagtttat gatccacaac agcaggcgat ggtgatcatt tcgctgtttc cttatgatcc
     2221 acaactctgc tttgaacact tctcgattca gatggcaccc ggtgcaataa gcgaatcgac
     2281 gccccatgag aagggggtaa tcgaacatgt tgtcgtcatt gatggacaac ttgatctgtg
     2341 cgttgatggt gaatggcaga cccttaattg tggggaaggc gttcgatttg ctgcagacgt
     2401 cacgcatatc tatcgtaatg gtggggagca aaccgtacat tttcattccc tcatccatta
     2461 cccgcgcagt taagcgggaa aactatttcg caacgtcgcg cttgtggcta aaatagccgc
     2521 catttttcag ctactggata agaatgtgac cgtatcttct catcgacttg aactgttaag
     2581 cccggcacgc gatgccgcca ttgcccgcga agctattttg cacggtgccg atgctgttta
     2641 tatcggcggc cctggttttg gtgcccgtca taatgccagt aatagcctga aagatattgc
     2701 cgagctggtg ccgtttgccc atcgttatgg tgcaaaaatt ttcgtcacgc ttaacaccat
     2761 tttgcatgat gatgagctgg aacccgcgca acggctgatt actgacctct accagaccgg
     2821 tgtcgatgcg ctgattgttc aggatatggg gattctggaa cttgatattc cgccgattga
     2881 actgcacgcc agtacgcagt gcgacattcg tacagtagaa aaagcgaagt tcctctctga
     2941 tgttggcttc acgcagattg tgctggcgcg agagctgaat cttgatcaga tccgcgcgat
     3001 tcaccaggcc acggacgcga ccattgaatt ctttattcat ggcgcactgt gcgtggccta
     3061 ttcgggtcag tgctacattt ctcatgcgca aacagggcgt agcgccaacc gtggcgattg
     3121 ctcgcaagcg tgccgtttgc catacacatt gaaagacgat caggggcggg tggtttccta
     3181 tgaaaaacat ctgctgtcga tgaaagataa cgatcagact gccaacctcg gcgcgctgat
     3241 tgatgctggc gtacgttcct tcaagattga agggcgttac aaagatatga gctacgtgaa
     3301 gaatatcacc gcccattatc gccagatgct tgatgccatt attgaagaac gtggcgatct
     3361 ggcgcgcgct tcatcaggtc gtactgaaca tttctttgtt ccatcgacgg aaaagacttt
     3421 ccaccgtggt agcacagatt attttgtgaa tgcccgtaaa ggcgatattg gcgcgttcga
     3481 ttcgccgaaa tttatcggcc tgccggtagg tgaagtagtg aaagtggcga aagatcatct
     3541 cgatgttgcc gttaccgagc cactggcaaa tggcgatggc ctgaacgtgt tgattaaacg
     3601 tgaagtcgtc ggttttcgtg ccaatacggt cgagaaaacc ggagaaaatc agtaccgcgt
     3661 ctggcccaat gaaatgccag cagatttgca caaaattcgc ccgcatcacc cactaaaccg
     3721 taatcttgat cataactggc agcaggcact gacaaaaacc tctagcgaac gtcgggtggc
     3781 ggtagacatt gaactgggcg gttggcagga acaattgatt ctgactctca ccagtgaaga
     3841 gggggtcagc ataacgcata cgctggatgg gcagttcgac gaagccaata acgcagaaaa
     3901 agcaatgaac aatctgaagg atggtctggc aaaactgggg caaaccctct attacgcccg
     3961 cgatgtgcaa attaatttgc cgggggcgct gtttgtacca aacagtctgt taaaccagtt
     4021 ccgccgtgaa gctgccgaca tgctggatgc tgcgcgtctt gccagttacc agcgcggcag
     4081 ccgtaaaccg gttgctgatc ctgcgccggt ttatccgcaa acacatctga gtttcctcgc
     4141 gaacgtttac aaccagaaag cgcgtgaatt ttatcatcgc tatggtgtgc agctgattga
     4201 cgcggcgtat gaggcacatg aagagaaggg cgaagtcccg gtgatgatca ccaagcattg
     4261 tctgcgcttt gcctttaatc tgtgcccgaa acaggcgaaa ggcaatatca aaagctggaa
     4321 ggcgacgcca atgcaactgg ttaacggcga tgaagtatta acgctaaagt ttgattgccg
     4381 cccatgcgag atgcacgtca ttggcaaaat caaaaatcac atactgaaaa tgccgttacc
     4441 gggaagcgta gtggcatccg taagtccgga tgagctgctg aaaacattgc cgaagcgaaa
     4501 agggtaaaac gccagttttc tggttactca caacttattg aatctgcatg atattgcctg
     4561 ccgggtaagg cgttacgccg catccggcat caaacgactc aacgttgtcc cgcttctggt
     4621 ttgcgcgatt tttgccagta aaaatgctcg cgcaaacctt ccgccgactc ttccgccaca
     4681 gcacgcaatt catcgctgtc cgcttcatga cgcagctgat gatccacatt ctttacccac
     4741 acaaattcat gtcctttgtg ccctgccatg agttgtcctg agaacagcgc acacgttaat
     4801 aagacaaccg ataacgcctt cgtaaacatc ctgccacctt tttcttacct tttgccgcta
     4861 tgatgccgat cgtttcttga ggttattatt cagttttgca aattagcgca aagaaattct
     4921 ggaatcttcc ttcctgattt tgcattgcat tctgccgttg cggcgattta gtgctatttt
     4981 cgagcaaatt acacacggag gtaaacacta atgtttactt tgttgatata ctcagccgca
     5041 gggaggcgat gtgaaacaaa gcgagttcag acgttggctc gaatctcagg gcgtcgatgt
     5101 agcgaatggc agcaaccatt tgaaactcag gtttcatggg aggcgcagtg tcatgccgcg
     5161 tcacccctgc gatgagatta aagaaccatt gcgtaaagca atcctgaaac aactcggttt
     5221 gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa ttatgcgtta
     5281 tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg tggatatccc
     5341 tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa aagatgcttt
     5401 actgaccgca tttgattttt attttgaaga taacgagctt atccctttac cttcgccatt
     5461 aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaagg tattgctgtt
     5521 aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa ttggcaaacc
     5581 taaacaagag attactcgcc tatttaactt gcatcatgcg acaaaaatcg acgcagtcca
     5641 gctcgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat tacagttaac
     5701 gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac ataattacga
     5761 ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga ttgcgtcggg
     5821 tatctggcaa cccggcgatc gtttaccttc gttgcgtgac caggtggcgc tttctggcat
     5881 gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat atattatcgc
     5941 acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga aagtgccagt
     6001 cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg atatgttgca
     6061 ggccagtcgc gatccgtcgg tcgttccgtt tgcctcggcc tttcccgacc cgcgactttt
     6121 ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca cggcgatgag
     6181 cgtgattgaa aacttgccgc caggaaacgc agaactgcgt caggctattg cccgtcgcta
     6241 tgccttacag gggatcacca tttctcctga tgaaattgtc atcactgccg gggcgttaga
     6301 ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga tagtagagaa
     6361 tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg cgttatcggt
     6421 ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt tgcaggagta
     6481 tccggtgaaa gcgtgctggc tgatgactaa tagccagaat ccactcggat ttaccttaac
     6541 gccgcaaaaa aaagcacaac tggtggcgtt gctcaatcag tacaacgtaa cgctgattga
     6601 agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg cgaaagcgtg
     6661 ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg tgcctggttt
     6721 tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct tgcagctgat
     6781 gagtacgctt tccaccagct caccgatgca gcttgcgctg gtggattacc tttccacgcg
     6841 ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta aacaacgtgc
     6901 ctggcaggca ctgctgcgtt atctgcctgc ggaagtgaaa attcatcata atgacagcgg
     6961 ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattga gcctggcggc
     7021 actgacgcat catatcagta ttgcgccagg taaaatgttt tctaccggtg aaaactggtc
     7081 acgttttttt cgttttaata ccgcgtggct gtggggagaa cgtgaagaac aggcggtaaa
     7141 acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat tcctcttaga
     7201 ttgggtaata tgaatttcga atagcagtca tatttcctaa ctccttgtct atactccaga
     7261 agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa ttttctcagg
     7321 ggcgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt tttacggcac
     7381 ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca tttgcccgca
     7441 gcagcctgtg tgcgctcagc atgacaataa tgaccgctca cgccgccgaa ccgcctacca
     7501 atttagataa accggaaggg cgactggata ttatcgcctg gccgggatat atcgaacgcg
     7561 gacaaactga taaacaatac gactgggtaa cgcaattcga aaaagagaca ggctgcgcgg
     7621 tgaatgttaa aaccgccgcg acttccgatg agatggtcag tctgatgacc aaagggggtt
     7681 acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggt aaacgcgtgc
     7741 agccgattaa taccgccttg attcccaact ggaaaacgct tgatccgcgc gtggttaaag
     7801 gcgactggtt taacgttggc ggcaaagttt acggcacacc ttaccaatgg gggccgaacc
     7861 tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa gtggtttttg
     7921 ttgagcaaaa tctgcaggac ggcaagagca ataaaggccg cgttcaggct tatgatggcc
     7981 ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag ttgggcatca
     8041 gcgatccgta tcaactcacc gaagaacagt accaggcggt gctgaaagtg ctgcgtgatc
     8101 aacatagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc gatttcaaaa
     8161 acgagggtgt ggtcgcttcc agtgcctggc cctatcagac caacgccctg aaagccgaag
     8221 gtcagcctgt cgctaccgtt ttcccgaagg agggcgttac cggttgggct gacaccacca
     8281 tgctacatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac tggtcattaa
     8341 cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccagtc gtgccggaag
     8401 ggtgtaaagc cagttcgtta ttaggcgaga aaggttgtga aacaaacggt tttaactatt
     8461 tcgataaaat agccttctgg aaaacgccta tagcagaagg gggcaagttt gttccctaca
     8521 gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc tggggtgctt
     8581 tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg tgcgcgcagt
     8641 agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg ggccgtccgg
     8701 ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt ccggcggggc
     8761 tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg acgtgaatac
     8821 tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg tcgcctatgg
     8881 gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag aggcgctgga
     8941 gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg gtggtcagcg
     9001 ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc tgttggatga
     9061 accgctcggc gcactggatc tcaaattgcg tgagcagatg cagcttgagc tgaaaaaact
     9121 gcaacagtct ctcggtatca cttttatctg cgttacccac gatcagggcg aagcgttatc
     9181 gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg attccccgcg
     9241 cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta catcgaatgt
     9301 ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg ccctgcggcc
     9361 ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga tccaggcggt
     9421 gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg aaaaactgct
     9481 ggtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc ccggacaaca
     9541 ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga ggtgaatggc
     9601 gatgaacgta ttgcaatcac cttcacgtcc aggtctgggt aaggtgtccg gtttcttctg
     9661 gcggaatccg gggctggggc tgtttttgct gctgcttggc ccgctaatgt ggtttggcat
     9721 tgtctatttc ggctcgctgc taacgctgtt atggcagggc ttttatacct ttgacgattt
     9781 caccatgtcg gtaacgccgg aactgacgct ggcgaacatc cgtgcgctgt ttaatccggc
     9841 gaattacgac atcattctcc gcacgctgac tatggctgtg gcagtcacta tcgccagcgc
     9901 cattctggct tttccaatgg cgtggtatat ggcgcgctat accagcggga aaatgaaagc
     9961 gtttttttat attgcggtaa tgttgccgat gtgggcgagc tacattgtta aagcctatgc
    10021 ctggacgtta ttgctggcaa aagatggcgt ggctcagtgg tttttacaac atcttgggct
    10081 ggaaccactg ctgacagcgt tccttacatt acctgcggtg ggcggcaata cgctgtcaac
    10141 ttccgggctg gggcgctttc tggtgtttct ctatatctgg ctgccgttca tgatcctgcc
    10201 cgtgcaggcg gcgcttgagc gtttgccgcc gtcattgttg caggcgtcag ctgatctcgg
    10261 cgcacgtcca cgacaaacct ttcgctatgt ggtgttgccg ctggcgatcc cgggtattgc
    10321 cgctggctct atctttacct tctcactcac actgggcgat tttatcgtcc cgcagctggt
    10381 tggcccgcca ggatatttta tcggcaatat ggtttattcc cagcaggggg cgattggcaa
    10441 tatgccgatg gcggcggcat tcaccctggt gccgattgtt cttatcgcac tgtacctggc
    10501 gttcgtgaaa cgtctgggag cgtttgatgc actctgaacg cgcaccgttt ttcctcaaac
    10561 tggcggcctg gggcggcgtt gttttcctac attttcccat cctgataatc gccgcctatg
    10621 cgtttaacac tgaagatgcg gcgtttagtt ttccaccgca gggcctgacg ctgcgctggt
    10681 ttagcgtggc agcacagcgt agtgatattc ttgatgccgt gacactgtca cttaaagtgg
    10741 cggcgctggc gacattaatg gcgctggtgt tagggacgct ggcagctgcc gcgctgtggc
    10801 gacgagactt tttcggcaaa aacgccattt cgctgttact gctgctgccc attgcgctgc
    10861 cgggcattgt cactggtctg gcgttattaa ccgcctttaa aaccatcaat ctggagccgg
    10921 gatttttcac catcgtggtc ggtcatgcga ctttttgtgt agttgtggtg tttaacaatg
    10981 tcatcgcccg ttttcgccgc acctcatgga gtctggttga ggcgtcaatg gatcttgggg
    11041 ccaatggctg gcaaaccttc cgctacgtag tgttgccgaa tctcagttcg gcgttactgg
    11101 caggaggaat gctggcgttt gccttgtcgt tcgatgaaat catcgttacg acctttacgg
    11161 caggtcatga acgaacgtta ccgttgtggt tgctcaatca gcttgggcga ccgcgtgatg
    11221 taccggtaac taacgtggtg gcactgctgg ttatgttggt aacaaccttg ccgatcctgg
    11281 gagcctggtg gctaacccgc gaaggcgaca atggtcaata accactgata caggaatatg
    11341 ctatgcaaca taagttactg attaacggag aactggttag cggcgaaggg gaaaaacagc
    11401 ctgtctataa tccggcaacg ggggacgttt tactggagat tgccgaggca tccgcagagc
    11461 aggtcgatgc tgctgtgcgc gcggcagatg cagcatttgc cgaatggggg caaaccacgc
    11521 cgaaagtgcg tgcggaatgt ctgctgaaac tggctgatgt tatcgaagaa aatggtcagg
    11581 tttttgccga actggagtcc cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg
    11641 aaatcccggc gattgtcgat gtttttcgct ttttcgcggg tgcggcgcgc tgtctgaatg
    11701 gtctggcggc aggtgaatat cttgaaggtc atacttcgat gatccgtcgc gatccgttgg
    11761 gggtcgtggc ttctatcgca ccgtggaatt atccgctgat gatggcagcg tggaaactgg
    11821 ctccggcgct ggcggcaggg aactgcgtag tgcttaaacc atcagaaatt accccgctga
    11881 ccgcgttgaa gttggcagag ctggcgaaag atatcttccc ggcaggcgtg attaacgtac
    11941 tgtttggcag aggcaaaacg gtgggcgatc cgctgaccgg tcatcccaaa gtgcggatgg
    12001 tgtcgctgac gggctctatc gccaccggcg agcacatcat cagccatacc gcgccctcca
    12061 ttaagcgtac tcatatggaa cttggtggca aagcgccagt gattgttttt gatgatgcgg
    12121 atattgaagc agtggtcgaa ggtgtacgta catttggcta ttacaatgcc ggacaggatt
    12181 gtactgtggc ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac
    12241 tcggtgctgc agtggcaacg ttaaaatctg gtgcgccgga tgacgagtct acggagcttg
    12301 gacctttaag ctcgctggcg catctcgaac gcgtcagcaa ggcagtagaa gaggcgaaag
    12361 cgacagggca catcaaagtg atcactggcg gtgaaaagcg caagggtaat ggctattact
    12421 atgcgccgac gctgctggct ggcgcattac aggacgatgc catcgtgcaa aaagaggtat
    12481 ttggtccagt agtgagtgtt acgctcttcg acaacgaaga acaggtggta aactgggcga
    12541 atgacagcca gtacggactt gcatcttcgg tatggacgaa agatgtgggc agggcgcatc
    12601 gcgtcagtgc acggctgcaa tatggttgta cctgggtcaa tacccatttc atgctggtaa
    12661 gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg caaggatatg tcactttatg
    12721 ggctggagga ttacaccgtc gtccgccacg tcatggttaa acattaagga taatattgca
    12781 gatcgtaaga gtatgagatg atcttgcgta ctgtcagagc actgatttct ctgacagtac
    12841 gtgctgcgtg caaaatcact acatcaaata aaccagccaa atctcataga gataagagta
    12901 aacaaaccaa cagcagcaaa gacatttatc agtactacag ttctactgga aacattcatt
    12961 tttgccacct tttatcattg cccctatgtg tatagtacgg gttaagaaaa tccgcaataa
    13021 tactcattct gaagatggat aagggcaagt tgctgtttga tgtttttttc tcaaaccggt
    13081 taaaaaactt gatgcacgtc aaaaaatgac gcatatttgc gcgcgtttta ttcatctggc
    13141 tggacgcccg tacatgtctc tctatcaaca catgcttgtt ttttatgcgg ttatggcagc
    13201 aatcgcattt cttatcactt ggtttctttc tcacgataag aaacgcatcc gtttcttaag
    13261 cgcctttctg gtgggggcaa catggccgat gagtttcccg gtggcgctgt tgttttcact
    13321 gttttaaatc ttactcactg gcttgacggc accacgcaga cttatatcat ttggatgaat
    13381 cgataaattt cacaagtggc taaggagaaa gtatgtcgca tctggatgaa gtcatcgcgc
    13441 gcgtggatgc cgcgattgaa gagagcgtca ttgcccatat gaacgaatta ttaatcgccc
    13501 tgagcgatga cgcggagtta agtcgggaag atcgctacac ccagcagcag cgtttgcgca
    13561 ccgctattgc tcatcacggt cgcaagcata aagaagatat ggaagcgcgc catgaacagt
    13621 taaccaaagg cggcaccatc ctgtgattaa aatgagcgtc tggctaccaa ccaggcgcca
    13681 accacaagta aaattgcgcc acaaattggg ccgaacaacg cccgtagtgg tacgccatga
    13741 ctgcggaaga tatccagaac cagaccgcca atcagctggc tggcgaccag tactgcaata
    13801 gttgtcgccg ctcccacatt ctgatatccg ctgatactgg cgaagacaaa aaacgatccc
    13861 agcaagccag gtataagtgt ccaccagcgc acgctggaca ccagttcgcc aaaccccgcc
    13921 ataccttgtt taaaccataa aatagaaaca aagagcacaa tccccaccag tgagttcagc
    13981 aacatggcga tgagaatggt agaggaggtc tgggtgatgc gcaccatcag cgagttctgg
    14041 actaccagac caattccggc ggcaatcaaa aaggcaaggg tgagcgactg attcatccaa
    14101 tcgcgtccgg ttcagtgcgt tcgtcgagtt gcaactgcat aaatgtcaga tccagccagc
    14161 gcccaaattt agtgcccacc tgcggcattt gtgcggtgac gacaaatccc agcgactggt
    14221 gcagatgcag cgaggcctga ttttgcgatt cgatcccggc gaccatgaca tgcttcccgc
    14281 aatctcgcgc ttcatcaatc aatcggctta acaatttacg ccccagacct ttgccctgat
    14341 gatcgggatg gacataaacc gaatgttcca cggtatggcg aaaaccatcg aaactacgcc
    14401 agtcgccaaa tgaggcatat cccgtcacta cgccatcttc ttcacttacc agcaccggat
    14461 aacctgctat ggtccgcgct tcaaaccagg caatgcggtt atctgcatcc accgtttggt
    14521 cattccagat agccgccgta tacaacacgg cgtggttata aatttccgca atggcagcac
    14581 agtcggcttt gcgggcaaaa cggatggaca tgtctggcct caataaaata atgatgatga
    14641 tgttatcaat actatcgaaa acagcccgaa gcaacggatt cctcgatgaa attattgcgt
    14701 gttgcagagg gggggtgacg gctgcaaaca aaaccaatcc tgcgagcctg ctactattga
    14761 tggattcatg ccaaaagcac aggagagcat tatggggcaa caaaagcagc gtaatcgacg
    14821 ttgggttctg gcctcgcgtc cacatggcgc accggttccg gagaatttcc gtcttgaaga
    14881 agatgatgtc gccacaccgg gtgaaggaca ggtgttactg cgcacagttt atttgtccct
    14941 ggacccgtat atgcgtggac gtatgagcga tgagccatct tattcaccgc cggttgatat
    15001 tggcggcgtg atggtcggcg gtacggtgag ccgtgtcgtg gagtcgaatc atcctgatta
    15061 tcagtctggc gactgggtgc tgggctacag tggatggcaa gactatgaca tatccagtgg
    15121 tgatgatctg gtgaaacttg gcgatcatcc gcaaaatcca tcgtggtcgc tgggtgtgct
    15181 agggatgcca ggctttaccg cttatatggg cctactggat atcggtcagc ctaaagaggg
    15241 cgaaacgttg gtggtagctg cggcgacagg accagtgggg gcgacggtgg ggcaaatcgg
    15301 caaacttaaa ggttgcagag tggtgggggt agccggtggc gcggaaaaat gccgccatgc
    15361 taccgaggtg ttaggcttcg atgtttgtct tgatcaccac gcggatgatt ttgccgaaca
    15421 actggcgaaa gcgtgcccaa aaggtattga tatctattat gaaaacgtgg gcggtaaggt
    15481 attcgatgcg gtgctaccgt tacttaatac atctgcgcgc attcccgtct gcggattagt
    15541 gagcagctat aacgctacag agctaccacc cggtccggat cgtttacctc tgttgatggc
    15601 tacagtgctg aaaaaacgta ttcgcttgca aggttttatt atcgctcagg attatggtca
    15661 ccgcatccat gagtttcaga gggagatggg gcaatgggtg aaagaggata aaatccacta
    15721 ccgcgaagaa attactgacg gtttagagaa tgcgccacag acgtttatcg gcctgctgaa
    15781 gggtaaaaac ttcggcaaag tggtgatccg cgtggcgggt gatgattaaa gtttcaacgg
    15841 cggcgtaagc cgccataaaa atccaggcaa aaagcttatt ttcagcttta attaactaac
    15901 cacatcattg ttctgctgct aataacaaca aaggtgagtg gtttatgatt attcacttta
    15961 atacaccagg tgaattcctt ctgccatgca ggcagggttg gacagaaaac acgcgaagga
    16021 attaaaatgc ctggaacgga aaaaatgaaa catgtcagtt tgactctgca ggttgagaac
    16081 gacctgaaac atcagcttag tattggcgca ctaaaacctg gcgcacgcct gattactaaa
    16141 aatctggcgg agcaattagg tatgagtatt acacctgtgc gtgaagcatt attacgtctg
    16201 gtttcggtga atgcgctttc tgtcgcacca gcgcaagcat ttacagttcc ggaagtgggg
    16261 aaacgtcaat tagacgaaat caatcggatc cgctacgaac tggaattaat ggcagttgct
    16321 ctggctgttg aaaacctcac cccgcaagac ctggcggaac tccaggaatt gctcgagaaa
    16381 ttacagcagg cgcaagaaaa gggcgacatg gaacaaatca ttaatgtaaa caggctattt
    16441 cgcttagcga tttatcatcg ctcaaatatg cccatcctgt gtgagatgat tgagcaactg
    16501 tgggtcagga tgggacctgg tttacattat ctctatgaag cgattaatcc agcggaatta
    16561 cgggagcata tagaaaacta tcatctgtta ctcgctgcgt taaaagcaaa agacaaagag
    16621 ggatgcagac attgtcttgc tgaaattatg caacaaaata ttgctatttt ataccagcaa
    16681 tacaatcgtt aaagtaaatg cccggcaaat accgggcatc aaggtaatta ctcaaatcgc
    16741 catgcgatat tcacgccgac accataattt cgcccgggcg caggttcgta atatcgcccg
    16801 tttgactcat tgacaatgac agaaccaacg tattctttat cgaataaatt atcgacacga
    16861 ccaaacaaat ccacagttaa attgtggtaa ttgtatttat acccggtgaa taagccgacg
    16921 agagtataag acggcgcttt ggccgtattt tcatcatctg ccataatatc gcccatataa
    16981 cgcgcttcca tgcctgcata ccaaccatct tccggtacat aacctatcga cgcaaagccc
    17041 atattacggg cgatccctgg catccgatta ccgttacaat cctgttcgtt gcaaacattg
    17101 ctgcgatagg tcgcatcaag ccaggtccag gacgcgttta cgcgaaaatc tcccgcgaaa
    17161 cgttgatccc atgccagttc agcgccttga cgacgggtct ttccagcatt tttgtaagtc
    17221 gtacgcccac cgctactgct atcgacaaca atttcatcat cggtgtcggt ctggaacaat
    17281 gcgagactaa gcagcccatc accaatacgc gttttactgc cgatctcaat tgtatcgttg
    17341 gtggatggtt ttaaaccaaa gttcataccg ctttgcccat cagcgcgata agacagctca
    17401 ttaatcgtcg gcgtttcaaa accacgcccg gctgccagat agatattcca ggcatcggtc
    17461 attgcatatt ttaacgatcc ggctggtagc catttgtgat aactggcatc accgctgtca
    17521 tcgccgttac ccggagtaac gtaatggtcg ttggaatcaa accacacgga gctgtagcgc
    17581 acgccagcat ccagcgacag tttttcgctc agctgccact gcgtctgtaa atagggatcg
    17641 atgttccaca tcagattgcg ttcgtcgcga cgcaactcac ctttttgccc gtactccggc
    17701 atgccgctat tcaggcggaa gttattgtag cccttgcggt tttcactcat gttttcgtag
    17761 ttcaggccgg tagtgaacgt gaccggaacg cccagttcac cacggtgtgt ccagcggctg
    17821 tctattccct ggtaatggcg ttgcagggta atcacgctgc ccgcatgtga cgggttaagt
    17881 tgtggtgcca tgggtattga ctggtactgg gtcgtttctc gctctccggc atacatcatc
    17941 acactcatat catcccgcga actcaggcta cgttcatagc gcaacccagc ctgagtttgc
    18001 ttgatggttt ttcgcgtgtc gtactgttct gcacgaggcg cttgttgcgg attcgctttc
    18061 cattctgctt tggttagccc acctgggtca tctgctttga tatccacgct attgaaaatc
    18121 aggcttaatt tgctggcgtc atcaatgcgt acgcccagtt tggcattagc taaatttttc
    18181 tgtgcgccac tatggtcacg atagccgtgg gtcgtaaaac gcgtggttga gacggtgtaa
    18241 tcaacatcgc caggctgtgt gccgtctccc gttgcgcccg ttgctttcag cccatagcgc
    18301 cagctgccaa aactgccgta gtaactactg gcttcaatgg ttggtggctg ttgcccggtc
    18361 tgggtggtga cattcattac accgccagac gcgttgccat acagggcaga gaaggggcca
    18421 cgcagcactt ccacattttg cacactgctt aaatcgatgt tggatgtttg cccttgcccg
    18481 tcgggcatgg tagcgggaat accgtccaca tacaggcgaa taccgcgaat tccgtaagtg
    18541 gagcgggagc caaatccacg aatcgacagc tgtaaatctt gcgcatagtt ctgccggttt
    18601 tgtacctgca aaccaggcac gccggtcagt gattcggaca agttaatgcg cggtgttgcc
    18661 aggcgcatct cctcgccatc caccacgctt actgctgcag gggtatccag ttctgaaacc
    18721 acctgcggcg cggcactgac aatcatggtc tgttcatcag cggcaaaaac aacgggggaa
    18781 aggacaagca gggcgggcaa aacggtctgt cgaacgaaaa caatcttcat gaaaaaagcc
    18841 aggttaagaa tgggaaaaca ccgtcatggt aatgaaattg taaatttatg gaaaacgaaa
    18901 cggcacgata cgttaattaa ttgagagaat tgtagtcgta acggcaagaa atgctccaca
    18961 tttgagtaaa taatgattac cattcccatt tataacaaga gcgtaacgat gattacgctt
    19021 agcgaagcat tgtgaagcag caaaaatatc ggttcatcaa agggagtcgt catgcattta
    19081 cgtcatctgt tttcattgcg cctgcgtggt tcattactgt taggttcatt gcttgttgct
    19141 tcatcattca gtacgcaggc cgcagaagaa atgctgcgta aagcggtagg taaaggtgcc
    19201 tacgaaatgg cttatagcca gcaagaaaac gcgctgtggc tcgccacttc gcaaagccgc
    19261 aaactggata aaggcggcgt ggtttatcgt cttgatccgg tcactctgga agtgacgcag
    19321 gcgatccata acgatctcaa gccgtttggt gccaccatca ataacacgac tcagacgttg
    19381 tggtttggta acaccgtaaa cagcgcggtc acggcgatag atgccaaaac tggcgaggtg
    19441 aaaggccgtc tggtgctgga tgatcgtaag cgcacggaag aggtgcgccc gctgcaaccg
    19501 cgtgagctgg tagctgatga tgccacgaac accgtttaca tcagtggtat tggtaaagat
    19561 agcgtgattt gggtcgttga tggcgagaat atcaaactga aaaccgccat ccagaacacc
    19621 ggtaaaatga gtaccggtct ggcactggat agcaaaggca aacgtcttta caccactaac
    19681 gctgacggcg aattgattac catcgacacc gccgacaata aaatcctcag ccgtaaaaag
    19741 ctgctggatg acggcaaaga gcacttcttt atcaacatca gccttgatac cgccaggcag
    19801 cgtgcattta tcaccgattc taaagcggca gaagtgttag tggtcgatac ccgtaatggc
    19861 aatattctgg cgaaggttgc ggcaccggaa tcactggctg tgctgtttaa cccagcgcgt
    19921 aatgaagcct acgtaacgca tcgtcaggca ggtaaagtca gtgtgattga cgcgaaaagc
    19981 tataaagtgg tgaaaacgtt cgatacgccg actcatccga acagcctggc gctgtctgcc
    20041 gatggcaaaa cgctgtatgt cagtgtgaaa caaaaatcca ctaaacagca ggaagctacc
    20101 cagccagacg atgtgattcg tattgcgctg taatagatat ggcgagggtg ataacggaga
    20161 gacttgcctg actttgatca cgccgcatgc ggcacacctt cgtcagtaat ctaaatttgc
    20221 ccggcacgta gccgggcaat tttgcaatta gctcgccgtt tcaggcttaa acacaatttc
    20281 ctgtttttct tcatcttcct cgacgactgg cgcagtgctg tgaatttcag caacgcgttt
    20341 gcgcacacca aaccagccta taaccagcag aataccgata atcggcagcg ccgcgatagt
    20401 ataagtcccg ttcgggtaat cgaacgccat cagcacaagg acactcagta aaaacagtaa
    20461 tgtcagccag gaagtgaagg gcgcgccagg cagtttaaaa ctgacatccg ctgctttgcc
    20521 ttctttaatc gctttacgca ggcgcatctg gcacacgatg ataaacgccc atgaagcgat
    20581 gattcccagc gacgcgaagt tcaacacaat ctcaaatacg cgcgacggca ccagatagtt
    20641 gaggaatacg ccgacgacat acacaaccag tgtcgccaga atcccggcat acggcacatg
    20701 ctgacgactc attttcgcca taaaactcgg tgcggaaccg cccatcgcca ttgagcgcag
    20761 aatacgtccg gtgcagtaca gacctgaatt caggctggag agggcagcgg tcagcaccac
    20821 aatgttcata atgctgccga tatatggcac acccagttta gagaaaaacg tcacgaacgg
    20881 actttgcccc gcctgatacg cgctccacgg caataacata accagcaaca ccacggagcc
    20941 gacgtaaaac aggccaatac gccaaatcac actgttaatc gctttaggca ccatggtctg
    21001 cggatcttta cattctccgg cagctgtacc caccatttca atggaggcaa aagcaaacac
    21061 tacgccctga atcaacacca gcgcaggcag caaaccgtgg gggaagaagc cgccattatc
    21121 ggtgattaaa tgaaagccag tggtgttgcc atccagcggc tgaccactac cgaggaacac
    21181 cgtacccacg accaaaaagg tcacaatggc gagcacttta ataagcgcaa accagaactc
    21241 catctccgca aaccatttca caccgatcat attcatggtg ccaacgatgg taagtgcagc
    21301 gagcgcaaag acccactgcg gcacgccgcc aaacgcaccc cagtaatgca tatacagagc
    21361 gacggcggta atatcaacaa tccccgtcat cgcccagttg atgaagtaca tccagccagc
    21421 aacataagcg gctttctcac ccaaaaactc acgggcataa gagacaaaac tgccactgga
    21481 agggcggtgt agcaccagct cacccaatgc acgcagaata aaaaacgaaa acaagccaca
    21541 aattaaataa accagtgcca gtgctggacc cgccatttgc agtcgggctc ctgcacctaa
    21601 aaacaagccg gtgccaatcg cgccgccaat ggcgatcatc tgtacctggc gattgcccat
    21661 cgctttgtga tacccctctt cgtgggcatt aagccagcgg cgtttcgcgg cgtgttgatc
    21721 tgaagtgtcg gtgtcgtgtt tactcattgc tctccctgat tgctttaatg aaaaagtcat
    21781 ataagttgcc atgaacaatg tttattcatc ctggcgatga tagttatcac cattattctt
    21841 tatgggtata aaactatcgg cgcagcatcc tacccgttat cgataaacga tgcaaaacat
    21901 ccccttacaa tcctgaaggg gattaataca actgacgaaa aaatgacaaa tccttttgct
    21961 ggttaacctt tgtactgtcc tacatttaat ctttaaaaga ttgtgagggg catatgatta
    22021 aggtgtatgg cgtacccgga tggggctcta caatcagtga gctgatgcta acactggctg
    22081 atattcctta tcagttcgtt gatgtcagtg gttttgacca cgagggaact tcacgcgagt
    22141 tattgaaaac cctgaacccg ttgtgtcagg ttccgacact ggcactggaa aatgacgaga
    22201 ttatgacgga gacagcggcg attgcattga tggttctcga tcgtcgcccg gaccttgcgc
    22261 caccggttgg gcgtgccgaa cgtcagcagt ttcaacggtt attggtctgg ctggttgcta
    22321 atgtctatcc aacgttcact ttcgccgatt accctgagcg ttgggcatct gacgcacctg
    22381 aacagttaaa gaagaatgtt attgaatatc gtaaatcgct ttatgtctgg ctgaattcac
    22441 aattgaccgc tgaaccttat gcgtttggtg agcaactaac gctggtggac tgttatcttt
    22501 gcactatgcg cacatggggg cctgggcatg aatggtttca ggataatgcc acgaatatca
    22561 gtgcgattgc tgatgcagta tgtcagttac caacgttaca agaaatacta aaaaggaatg
    22621 aaattatttg agtgggatac cagaagatta tgttatttat tattcagtaa ctttatccac
    22681 aatgatgtgc gtaattaatt agattcatgt tatgtttttt aatttatatc acactccctt
    22741 tcattcagct tgtctatttc atttcctctg aaaagaaact aactattcac attgtgcaga
    22801 tgtttcactt gttatcacag
//
LOCUS       DAZFTN010000075        22396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFTN010000075 DAZFTN010000000
VERSION     DAZFTN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHKV2_003214"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHKV2_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO6941048.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHKV2_003215"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHKV2_003215"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO6941049.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHKV2_003216"
     CDS             complement(938..1405)
                     /locus_tag="ACHKV2_003216"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO6941050.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHKV2_003217"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHKV2_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO6941051.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHKV2_003218"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHKV2_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO6941052.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHKV2_003219"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHKV2_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO6941053.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHKV2_003220"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHKV2_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO6941054.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHKV2_003221"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHKV2_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO6941055.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHKV2_003222"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHKV2_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO6941056.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHKV2_003223"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHKV2_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO6941057.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHKV2_003224"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHKV2_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO6941058.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHKV2_003225"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHKV2_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO6941059.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHKV2_003226"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHKV2_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO6941060.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHKV2_003227"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHKV2_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO6941061.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHKV2_003228"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHKV2_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO6941062.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHKV2_003229"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHKV2_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO6941063.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHKV2_003230"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHKV2_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO6941064.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHKV2_003231"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHKV2_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO6941065.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHKV2_003232"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHKV2_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO6941066.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHKV2_003233"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHKV2_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO6941067.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHKV2_003234"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHKV2_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO6941068.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHKV2_003235"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHKV2_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO6941069.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHKV2_003236"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHKV2_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO6941070.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHKV2_003237"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHKV2_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO6941071.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHKV2_003238"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHKV2_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO6941072.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHKV2_003239"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHKV2_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO6941073.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHKV2_003240"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHKV2_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO6941074.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHKV2_003241"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHKV2_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO6941075.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHKV2_003242"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHKV2_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO6941076.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHKV2_003243"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHKV2_003243"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO6941077.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHKV2_003244"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHKV2_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO6941078.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHKV2_003245"
     CDS             15545..15913
                     /locus_tag="ACHKV2_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO6941079.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHKV2_003246"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHKV2_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO6941080.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHKV2_003247"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHKV2_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO6941081.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHKV2_003248"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHKV2_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO6941082.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKELNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHKV2_003249"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHKV2_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO6941083.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHKV2_003250"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHKV2_003250"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO6941084.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHKV2_003251"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHKV2_003251"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO6941085.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHKV2_003252"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHKV2_003252"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO6941086.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..>22396
                     /gene="dprA"
                     /locus_tag="ACHKV2_003253"
     CDS             21494..>22396
                     /gene="dprA"
                     /locus_tag="ACHKV2_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6941087.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSP"
BASE COUNT         5750 a         5328 c         5829 g         5489 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattcagctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcacca
//
LOCUS       DAZFTN010000076        22292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFTN010000076 DAZFTN010000000
VERSION     DAZFTN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1076)
                     /gene="espR3"
                     /locus_tag="ACHKV2_003254"
     CDS             complement(<1..1076)
                     /gene="espR3"
                     /locus_tag="ACHKV2_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFO6941088.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDCNLLNMLSEFKLLREQCFRWGNYTLLFENYEAYDKTGSITI
                     EKNQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKE
                     NVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISYIDSFPPKAKKIFICHNKLSEIPALPDTA
                     KVFDCSENNIKEIRWFPKNLKEAYIEYNKIEVIPAIPGNLKLLCMKCNPIKEAFLMPW
                     TLTGIRYEISQRKYIVMNPADYDK"
     gene            1260..1400
                     /locus_tag="ACHKV2_003255"
                     /pseudo
     CDS             1260..1400
                     /locus_tag="ACHKV2_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1425..1739)
                     /gene="fliE"
                     /locus_tag="ACHKV2_003256"
     CDS             complement(1425..1739)
                     /gene="fliE"
                     /locus_tag="ACHKV2_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6941089.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1954..3612
                     /gene="fliF"
                     /locus_tag="ACHKV2_003257"
     CDS             1954..3612
                     /gene="fliF"
                     /locus_tag="ACHKV2_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6941090.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3605..4600
                     /gene="fliG"
                     /locus_tag="ACHKV2_003258"
     CDS             3605..4600
                     /gene="fliG"
                     /locus_tag="ACHKV2_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6941091.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4593..5279
                     /gene="fliH"
                     /locus_tag="ACHKV2_003259"
     CDS             4593..5279
                     /gene="fliH"
                     /locus_tag="ACHKV2_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6941092.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5279..6652
                     /gene="fliI"
                     /locus_tag="ACHKV2_003260"
     CDS             5279..6652
                     /gene="fliI"
                     /locus_tag="ACHKV2_003260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6941093.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6671..7114
                     /gene="fliJ"
                     /locus_tag="ACHKV2_003261"
     CDS             6671..7114
                     /gene="fliJ"
                     /locus_tag="ACHKV2_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6941094.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7111..8238
                     /gene="fliK"
                     /locus_tag="ACHKV2_003262"
     CDS             7111..8238
                     /gene="fliK"
                     /locus_tag="ACHKV2_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6941095.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8343..8807
                     /gene="fliL"
                     /locus_tag="ACHKV2_003263"
     CDS             8343..8807
                     /gene="fliL"
                     /locus_tag="ACHKV2_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO6941096.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8812..9816
                     /gene="fliM"
                     /locus_tag="ACHKV2_003264"
     CDS             8812..9816
                     /gene="fliM"
                     /locus_tag="ACHKV2_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO6941097.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9813..10226
                     /gene="fliN"
                     /locus_tag="ACHKV2_003265"
     CDS             9813..10226
                     /gene="fliN"
                     /locus_tag="ACHKV2_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO6941098.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10229..10594
                     /gene="fliO"
                     /locus_tag="ACHKV2_003266"
     CDS             10229..10594
                     /gene="fliO"
                     /locus_tag="ACHKV2_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO6941099.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10594..11331
                     /gene="fliP"
                     /locus_tag="ACHKV2_003267"
     CDS             10594..11331
                     /gene="fliP"
                     /locus_tag="ACHKV2_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6941100.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11341..11610
                     /gene="fliQ"
                     /locus_tag="ACHKV2_003268"
     CDS             11341..11610
                     /gene="fliQ"
                     /locus_tag="ACHKV2_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO6941101.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11618..12403
                     /gene="fliR"
                     /locus_tag="ACHKV2_003269"
     CDS             11618..12403
                     /gene="fliR"
                     /locus_tag="ACHKV2_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6941102.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12688..13311
                     /gene="rcsA"
                     /locus_tag="ACHKV2_003270"
     CDS             12688..13311
                     /gene="rcsA"
                     /locus_tag="ACHKV2_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO6941103.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13355..13543)
                     /gene="dsrB"
                     /locus_tag="ACHKV2_003271"
     CDS             complement(13355..13543)
                     /gene="dsrB"
                     /locus_tag="ACHKV2_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO6941104.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13706..13933
                     /gene="yodD"
                     /locus_tag="ACHKV2_003272"
     CDS             13706..13933
                     /gene="yodD"
                     /locus_tag="ACHKV2_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO6941105.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14231..15046
                     /gene="yedP"
                     /locus_tag="ACHKV2_003273"
     CDS             14231..15046
                     /gene="yedP"
                     /locus_tag="ACHKV2_003273"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO6941106.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15043..16737)
                     /gene="dgcQ"
                     /locus_tag="ACHKV2_003274"
     CDS             complement(15043..16737)
                     /gene="dgcQ"
                     /locus_tag="ACHKV2_003274"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO6941107.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(16908..17090)
                     /gene="yodC"
                     /locus_tag="ACHKV2_003275"
     CDS             complement(16908..17090)
                     /gene="yodC"
                     /locus_tag="ACHKV2_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO6941108.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17169..18086)
                     /gene="yedI"
                     /locus_tag="ACHKV2_003276"
     CDS             complement(17169..18086)
                     /gene="yedI"
                     /locus_tag="ACHKV2_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO6941109.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            18259..19179
                     /gene="yedA"
                     /locus_tag="ACHKV2_003277"
     CDS             18259..19179
                     /gene="yedA"
                     /locus_tag="ACHKV2_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO6941110.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19168..19638)
                     /gene="vsr"
                     /locus_tag="ACHKV2_003278"
     CDS             complement(19168..19638)
                     /gene="vsr"
                     /locus_tag="ACHKV2_003278"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO6941111.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19619..21037)
                     /gene="dcm"
                     /locus_tag="ACHKV2_003279"
     CDS             complement(19619..21037)
                     /gene="dcm"
                     /locus_tag="ACHKV2_003279"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO6941112.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21104..21799)
                     /gene="yedJ"
                     /locus_tag="ACHKV2_003280"
     CDS             complement(21104..21799)
                     /gene="yedJ"
                     /locus_tag="ACHKV2_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO6941113.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(21839..22119)
                     /gene="drpB"
                     /locus_tag="ACHKV2_003281"
                     /pseudo
     CDS             complement(21839..22119)
                     /gene="drpB"
                     /locus_tag="ACHKV2_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5458 a         5842 c         5632 g         5360 t
ORIGIN      
        1 tatttatcat aatcggcggg attcataaca atatattttc gctgcgatat ttcatagcga
       61 atccctgtaa gggtccatgg cattaaaaat gcctctttaa taggattaca tttcatacaa
      121 agtaatttta aattgccagg tatcgcagga ataacctcaa tcttattata ttcaatatac
      181 gcttctttca aatttttggg gaaccatcta atttctttaa tattattctc actacaatca
      241 aaaaccttag cggtgtctgg tagtgctggt atttccgata gcttattgtg acaaataaaa
      301 attttcttag cctttggcgg aaatgagtca atatacgata tcaggttatt atttacggag
      361 atcagggtaa ttccatcagg tacttgtgga aattctgtaa ggttattgaa atcgagttct
      421 aattctttgc acgattcggg cacctgtatt gaatcaagtt cattataaac aaggctaagc
      481 atttcaatcc aatctggaag aggcggcaca gaacccaaaa ataggtatga taagtcaagt
      541 tcattactgt gaagcttaac acatttaaac attctcacca acgctctatc tacattttct
      601 ttgaggtcat tcgatccgcc ttctttgact gaactagccc agtcactgaa gcctttgcaa
      661 agtctggcat ctgtaatttt cgttaacttg tccagcaact ctgcaatatt agtcgatata
      721 aactctaatt tatgccgaat gggtaaagtc ccctccccct gatttttttc tatcgtgata
      781 gaccccgtct tatcataagc ctcataattt tcaaacaata gagtataatt accccacctg
      841 aaacattgct ctctcaataa cttaaattca gacagcatat tcagtaagtt gcagtcttca
      901 ctaactggtg taaacttatc atttccaaga caactgacta actcacttga taattcattt
      961 atgattgcat catcagatac taactcgtgt tgttttatcg tagactcagt attcgatgtg
     1021 attttattta attgaaccga ctgagttttt aacgacagat catttaatgg aaacatttac
     1081 actcctgttt aataaattat attgcccagg gcgcattcta aaacacaata aaattaccac
     1141 tatcaaaaat cgctcatatc catagcccgg aacatgaata aaaacatcaa caaagataaa
     1201 attatccaga ggttactgtt gcgtctgtgg tcgtaacaac atccgacgtg gtgatgtgtc
     1261 aaatgagaca ctctgatgtt cagggggttt atcgtgtata cggatcgtgg atggcagaaa
     1321 actttcagga tcaggtatcc atttcaaacc agataatgag taagtttgcc ccatccatgc
     1381 cccacgcggt gagatcggat gtcattaaca acaggttaca caacctatac ctgcatgctc
     1441 attacttcct gatatgccgc caccagctta ttacgcacct gaatccccat ttgcatagaa
     1501 actgaggctt tttgcatatc ggtcatcaca tcgtttaacg ccacgccggg ttcaccgaga
     1561 gtgaattttt ctgcctgcgt gcgggcggct gtttgtgtat cgcttatgcg atcgagcgcg
     1621 gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg cgcacgcgca
     1681 ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat cgctgacatt
     1741 ttcatctcct ggataatttc tggtagcaaa gcctaccagt aagtcaataa gacaaaggcg
     1801 ctaaatagca acaaaaaaac gggtttattg gcggatagaa aaaaacgaaa gcacaaataa
     1861 tgggagcgtc aatttttcga gtttgctgac ccgggagtga gtcttgttcc actttgccaa
     1921 taacgccgtc cataatcagc cacgaggtgc gcgatgaatg cgactgcagc ccagacaaaa
     1981 tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttgccggt
     2041 tccgcggcag tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc
     2101 acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa
     2161 atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaagttcc ggcagataaa
     2221 gttcacgagc tgcgcctgcg cctggcgcaa caaggtttgc ccaaaggcgg cgcggtcggt
     2281 ttcgaactac tcgatcagga aaagtttggt atcagccagt tcagcgaaca ggtgaattat
     2341 cagcgggcgc tggaaggcga gctttctcgt accatcgaaa ctatcggccc ggtaaaaggg
     2401 gcgcgcgtac atctggcaat gccgaaaccg tctttattcg tccgtgaaca aaaatcccct
     2461 tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc
     2521 gccattgtgc atctggtttc cagcgccgtt gctggtctgc cgccgggaaa cgtcacgctg
     2581 gtggatcagg gcggacatct gttaacccag tccaatacca gctggcgcga tcttaatgac
     2641 gctcaattga aatatgccag cgatgtcgaa ggccgtattc agcggcgtat tgaagcaatc
     2701 ctgtcgccta ttgttggtaa cggtaatatt cacgcccagg tcacggcgca actggacttc
     2761 gccagtaaag aacaaacgga agaacagtat cgccctaatg gtgatgaatc tcaggcggcg
     2821 cttcgttcac gccagcttaa tgagagcgag caaagcggtt ccggttatcc gggcggcgta
     2881 ccgggggcgt tgtcgaatca accggctcct gcgaataacg cgccaatcag cacgcctccg
     2941 gcaaatcaaa ataaccgcca gcagcaggcg agcaccacca gcaatagtgg gccgcgtagc
     3001 acacagcgga atgaaaccag taactacgaa gtcgatcgca ctattcgtca taccaaaatg
     3061 aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca
     3121 gacggtaaac cattgcctct cagcaacgaa cagatgaaac aaattgaaga tctgacccgc
     3181 gaggcgatgg gcttttctga aaaacgcggt gactcgctca atgtcgttaa ctcgccgttc
     3241 aatagtagtg acgaaagcgg cggagaactg ccgttctggc aacagcaagc gtttatcgat
     3301 cagttactgg ctgccggtcg ctggttgctg gtactgctgg tggcgtggct gctgtggcgt
     3361 aaagcggtac gcccgcagct aacacgtcgc gctgaggcga tgaaagctgt acagcaacag
     3421 gcgcaggccc gcgaggaagt ggaagatgcg gtggaagtcc gcctgagcaa agacgaacaa
     3481 ctccaacaac ggcgcgctaa ccaacgtctg ggggcagaag tcatgagcca gcgtatccgt
     3541 gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg gataaataac
     3601 gatcatgagt aacctgacag gcaccgataa aagcgtcatc ctgctgatga ccattggcga
     3661 agaccgggcg gcagaagtgt tcaagcacct ctcccagcgc gaggtgcaaa ccctgagcgc
     3721 tgcaatggcg aacgtcacgc agatctccaa caagcagcta accgatgtgc tggcggagtt
     3781 tgagcaagaa gctgaacagt ttgccgcact gaatatcaac gccaacgatt atctgcgttc
     3841 ggtattggtc aaagctctgg gtgaggaacg tgccgccagc ctgctggaag atattctcga
     3901 aactcgcgat accgccagcg gtattgaaac gctcaacttt atggagccgc agagcgccgc
     3961 cgatctgatt cgtgatgagc atccgcaaat tatcgccacc attctggtgc atctgaagcg
     4021 cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg acgtgatgtt
     4081 gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg gcggaactga ccgaagtact
     4141 gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg tgagaacggc
     4201 agccgaaatt atcaacctga tgaaaactca gcaggaagaa gccgttatta ccgccgtgcg
     4261 tgaattcgac ggcgagctgg cgcagaaaat catcgacgag atgttcctgt tcgaaaatct
     4321 ggtggatgtc gacgatcgca gcattcagcg tctgttgcag gaagtggatt ccgaatcgct
     4381 gttgatcgcg ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc gcaatatgtc
     4441 gcagcgtgcc gccgatattc tgcgcgacga tctcgccaac cgtggtccgg tgcgtctgtc
     4501 gcaggtggaa aacgaacaga aagcgattct gctgattgtg cgccgccttg ccgaaactgg
     4561 cgagatggta attggcagcg gcgaggatac ctatgtctga taatctgccc tggaaaacct
     4621 ggacgccgga cgatctcgcg ccaccacagg cagagtttgt gcccatggtc gagtcggaag
     4681 aaaccatcat tgaagaggct gaacccagcc ttgagcagca actggcgcaa ctgcaaatgc
     4741 aggcccatga gcaaggttat caggcgggta ttgccgaagg tcgccagcaa ggtcataagc
     4801 agggctatca ggaaggactg gcccaggggc tggagcaagg tctggcagag gcgaagtctc
     4861 aacaagcgcc aattcatgcc cggatgcagc aactggtcag cgaatttcaa actacccttg
     4921 atgcacttga tagtgtgata gcgtcgcgcc tgatgcagat ggcgctggag gcggcacgtc
     4981 aggtcatcgg tcagacgcca acggtggata actcggcact gatcaaacag atccaacagt
     5041 tgttgcagca agaaccgtta ttcagcggta aaccacagct gcgcgtgcac ccggatgatc
     5101 tgcaacgtgt ggatgatatg ctcggcgcta ccttaagttt gcatggctgg cgcttgcggg
     5161 gcgatcccac cctccatcct ggcggctgta aagtctccgc cgatgaaggc gatctcgacg
     5221 ccagtgtcgc cactcgctgg caagaactct gccgtctggc agcaccagga gtggtgtaat
     5281 gaccacgcgc ctgactcgct ggctaaccac gctggataac tttgaagcca aaatggcgca
     5341 gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct accgggctgg tgctggaagc
     5401 caccggatta caattgccgc tcggcgcaac ctgtgtcatt gaacgccaga acggcagcga
     5461 aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt caacggctgt ttttaatgcc
     5521 gctggaggaa gtggaaggtg tcctgcccgg cgcgcgggtt tatgccaaaa acatttcggc
     5581 tgaaggactg caaagcggca agcagttgcc gctcggtccg gcgttattag gtcgcgttct
     5641 ggacggcagc ggtaaaccgc tcgatggcct gccctccccc gatacgacgg aaaccggtgc
     5701 gctgattacc ccgccattta acccgttgca acgtacaccg attgaacatg tgctggacac
     5761 cggcgtgcgc ccaatcaatg ccctgcttac cgttgggcgt gggcagcgta tggggctgtt
     5821 tgccgggtcc ggcgttggta aaagtgtgct gctggggatg atggcacgtt acacccgcgc
     5881 cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgc gaagtaaaag attttattga
     5941 gaatatcctc ggtgccgaag ggcgtgcacg atcagtggtg attgccgctc cggcggatgt
     6001 gtctccgctc ctgcgaatgc aaggtgccgc ctatgccacg cgcattgccg aagattttcg
     6061 cgatcgtggt cagcatgtgt tgctgattat ggactccctc acccgctacg cgatggccca
     6121 gcgtgaaatt gcgctggcga ttggcgaacc acccgccacc aaaggttatc caccgtcggt
     6181 gtttgccaaa ttaccggcac tggtcgagcg tgccggaaat ggcattagcg gcggcggctc
     6241 gattaccgcg ttttataccg tgcttactga aggcgatgac cagcaggacc ccattgcaga
     6301 ctccgcgcgg gcgatcctcg acggtcacat tgtgctgtct cgccgactgg cggaagccgg
     6361 gcactatccg gctatcgata ttgaagcgtc gatcagccgc gcaatgacgg cgttgatcag
     6421 tgagcaacat tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt ttcagcgtaa
     6481 ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc tcgacaaagc
     6541 catcgccctg tggccgcagc tggagggcta tttgcaacaa ggcatttttg aacgcgcgga
     6601 ctgggaagcg tctctccagg ggctggagcg tattttcccg acagtgtcat aacccaggag
     6661 ataacggcag atggcagaac atggcgcgct ggcgaccctg aaagatctgg cagaaaaaga
     6721 ggtagaggat gccgcgcgcc tgctgggtga aatgcgtcgc gggtgtcagc aggcggaaga
     6781 acagctcaaa atgctgatcg attatcagaa tgaatatcgc aataacctca acagcgatat
     6841 gagtgccggg atgaccagca accgctggat caactatcag cagtttatcc agacgctgga
     6901 aaaagccatt actcagcatc gccagcaact taatcagtgg acgcagaaag ttgacattgc
     6961 cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc tggcagacac tgcaggaacg
     7021 gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt
     7081 cgcccagcgc gccgccatga ggaaacctga atgattcgct tagcgccctt gattaccgcc
     7141 gacgttgaca ccaccacatt gcctggcggc aaagccagcg atgctgcaca agattttctc
     7201 gcgttgttga gcgaagcatt agcaggcgag acaactaccg acaaagcggc cccccagttg
     7261 ctggtggcaa cagataagcc cacgacaaaa ggcgagccgc tgatcagcga tattgtttcc
     7321 gacgcgcaac aagctaattt actgatccct gtggatgaaa caccgcctgt catcaacgac
     7381 gaacaatcca catcaacacc gttaaccacc gctcagacga tggcgttggc tgcggtggct
     7441 gacaaaaata cgacaaaaga cgaaaaagcg gatgatctga atgaagacgt caccgcaagc
     7501 ctgagcgccc tttttgcgat gttgccgggt tttgacaata cgcccaaagt gactgatgtg
     7561 ccgtcaaccg tgttaccggc agagaaacca acgctcttca caaaactgac ttctgcgcaa
     7621 ctcacaacag cacagcctga tgatgccccc ggcacaccag ctcagccatt aacaccgcag
     7681 gtagcagaag cccagagtaa agcggaagtc atcagcacgc cttcgccggt gactgctgcc
     7741 gccagcccgc taatcactcc acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt
     7801 ttgagtgcac cgctgggttc tcacgaatgg caacaatcat taagccagca tatttcgctg
     7861 ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc acccgcagga tttaggtgaa
     7921 gtgcaaatct ccctcaaagt ggatgataac caggcgcaaa tccagatggt ttcatcgcat
     7981 cagcatgtac gcgccgccct ggaagcagcg ctgccggtac tgcgtacgca gctggccgaa
     8041 agtggcattc agttagggca aagcaacatc agtggcgaaa gctttagtgg tcagcagcag
     8101 gccgcttccc aacaacagca aagccaacgc acagtaaacc atgaacctct ggcgggggaa
     8161 gacgacgata cgcttccggt tcccgtctct ttacaagggc gcgtaacagg caacagcggc
     8221 gttgatattt tcgcctaacg tcagaggtag caccgtaatc cgcgtctttt ccccgctttg
     8281 ttgcgctcaa gacgcaggat aattagccga taagcagtag cgacacagga agaccgcaac
     8341 acatgactga ttacgcgata agcaagaaaa gcaagcgatc gctttggatc ccgattctgg
     8401 tattcattac cctcgcggcc tgtgccagcg caggttacag ctactggcat tcgcatcagg
     8461 ttgctgctga cgacaaagcg cagcaacgcg tcgtgccctc cccggtcttc tatgcgctgg
     8521 atacctttac ggtcaatctg ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc
     8581 gcctgaaaga tgaagctacc cgctcgcggc tgagtgagta tttgccggaa gtccgtagcc
     8641 gtttgctgtt actgttttcg cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga
     8701 aaaacctgat tgccgagatt aaaaccacac tttccacccc gcttgttgcc gggcaaccga
     8761 aacaggatgt caccgacgtg ctgtataccg cttttattct gcgataacga catgggcgat
     8821 agtattcttt ctcaagctga aattgatgcg ctgttgaatg gtgacagcga agtcaaagac
     8881 gaaccgacag ccagtgttag cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa
     8941 cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca ttaatgagcg ctttgcccgc
     9001 cattttcgta tggggctgtt caacctgctg cgtcgtagcc cggatataac cgtcggggcc
     9061 atccgcattc agccgtacca tgaatttgcc cgcaacctgc cggtgccgac caacctgaac
     9121 cttatccatc taaaaccgct gcgcggcact gggctggtgg tgttctcacc gagtctggtg
     9181 tttatcgccg tggataacct gtttggcggc gatggacgct tcccgaccaa agtggaaggt
     9241 cgcgagttta cccataccga acagcgcgtc atcaaccgca tgttgaaact ggcgcttgaa
     9301 ggctatagcg acgcatggaa ggcgattaat ccgctggaag ttgagtacgt gcgttcggaa
     9361 atgcaggtga aatttaccaa tatcaccacc tcgccgaacg acattgtggt taacacgccg
     9421 ttccatgtgg agattggcaa cctgaccggc gaatttaata tctgcctgcc attcagcatg
     9481 atcgagccgc tacgggaatt gttggttaac ccgccgctgg aaaactcgcg taatgaagat
     9541 cagaactggc gcgataacct ggtgcgccag gtgcagcatt cacagctgga gctggtcgcc
     9601 aactttgccg atatctcgct acgcctgtcg cagattttaa aactgaaacc cggtgacgtc
     9661 ctgccgatag aaaaacccga ccgcatcatc gcccatgttg acggcgtccc ggtgctgacc
     9721 agtcagtacg gcactctcaa cggtcagtat gcgttacgga tagaacattt gattaacccg
     9781 attttaaatt ctctgaacga ggaacagccc aaatgagtga catgaataat ccggccgatg
     9841 acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt gagcgaacaa aaatcaacca
     9901 gcagcaaaag cgctgccgac gcggtgttcc agcaatttgg cggtggtgat gtcagcggaa
     9961 cgttgcagga tatcgacctg attatggata ttccggtcaa gctgaccgtc gagctgggcc
    10021 gcacacgaat gaccatcaaa gagctgttgc gtctgacgca agggtccgtc gtggcgctgg
    10081 acggtctggc gggcgaacca ctggatattc tgatcaacgg ttatttaatc gcccagggcg
    10141 aagtggtggt cgttgccgat aaatatggcg tgcggatcac cgatatcatt actccgtctg
    10201 agcgaatgcg ccgcctgagc cgttagtgat gaataaccac gctactgtgc aatcttccgc
    10261 gccggtttct gctgcgccac tgctgcaggt gagcggcgca ctcatcgcca ttattgccct
    10321 gatcctcgct gctgcctggc tggtaaaacg gttgggattt gccccgaaac gcactggcgt
    10381 taacggtctg aaaattagcg ccagtgcttc gctgggcgcg cgtgaacggg ttgtggtggt
    10441 cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca gggcaaatca atctgctgca
    10501 taaacttccc ccttcagcac caacagaaga gataccgcag accgattttc agtcggtcat
    10561 gaaaaatttg cttaagcgta gcgggagatc ctgatgcgtc gtttattgtc tgtcgcacct
    10621 gtccttctct ggctgattac gcccctcgcc ttcgcgcaac tgccgggtat caccagccag
    10681 ccgctgcctg gcggtggaca aagctggtcg ctcccggtgc agacgctggt gttcatcacc
    10741 tcgttgacgt ttattccggc aattttactg atgatgacca gtttcacccg catcatcatt
    10801 gtttttggtt tattgcgtaa cgcgctggga acaccctccg cgccacctaa ccaggtattg
    10861 ctggggctgg cactgttttt gacctttttt attatgtcac cggtgatcga caaaatttat
    10921 gtcgatgcgt accagccatt cagcgaagag aaaatatcaa tgcaggaggc gctggaaaaa
    10981 ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc gtgaggcaga tttagggttg
    11041 tttgccagac tggcgaatac cggcccgttg cagggacctg aagccgtgcc gatgcgcatt
    11101 ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat ttcagatagg cttcacgatt
    11161 ttcatccctt ttttgattat cgacctggtg atagccagcg tgttgatggc attggggatg
    11221 atgatggttc cccccgccac cattgctctg ccctttaaac tgatgctgtt tgtactggtg
    11281 gatggctggc aattgctggt cggttcgctg gcgcagagct tttacagcta gagaggcaaa
    11341 atgacacctg aatcggtcat gatgatgggg actgaagcga tgaaagtcgc gctggcactg
    11401 gctgccccgc tattgttggt agcgttggtc acgggcctta tcatcagtat tttgcaggcc
    11461 gccacgcaga ttaacgaaat gacgctgtcg tttattccga aaatcatcgc cgtatttatc
    11521 gccattatta ttgccggacc gtggatgctc aatctgttgc tggattacgt ccgcaccttg
    11581 ttcactaacc tgccgtatat catcgggtag ccgtactatg ttgcaggtga caagcgaaca
    11641 atggctatcc tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctgatctc
    11701 caccgcgccg attctgagcg aacgcagcgt accgaaacgg gtaaaactgg gtctggcaat
    11761 gatgatcacg ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt
    11821 ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg gttttaccat
    11881 gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct
    11941 gtcatttgcg acgtttgtcg atccggccag ccatcttaat atgcccgttt tagcgcgtat
    12001 catggatatg ctggcgttac tgctgttcct gacatttaac ggtcatttat ggttgatttc
    12061 actgctggtc gatacctttc acaccctgcc gattggtggc gaaccgttga acagcaatgc
    12121 gtttctggca ctcaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt
    12181 accgctcatt actctgctgc tgacactgaa tctggcatta ggtttactta atcgtatggc
    12241 cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt
    12301 aatggcggca ttaatgccgt taattgcgcc tttttgcgaa catttattca gtgaaatttt
    12361 taatttgctg gctgatatta ttagtgaatt gccattaata taattccgta acgtttatca
    12421 tgttatccta aggattatcc gaaaaataat acctacgaac atcttccagg atactcctgc
    12481 agcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttaa gacaattcca
    12541 ggcaaattat acaacacttt acgggatagt aagtccgcct gaaaaatcgc gagagtggcg
    12601 cattaggtga cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta
    12661 tcgttacgca ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac
    12721 acccgactag gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac
    12781 gacattgaaa ccgttgatga ccttgccata gcttgtgatt cacagcgccc ttcagtggtg
    12841 tttattaatg aggactgttt catccacgat gcttctaaca gtcagcgtat caagctcatc
    12901 attaatcaac atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttt
    12961 gatgaatacc tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa
    13021 tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta
    13081 aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca
    13141 ggtcagggaa ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg
    13201 cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc
    13261 gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg
    13321 actggtggtt tcccaccagt caggctgaat tagattactc tgctttctcc acaaagatac
    13381 cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgaaa
    13441 ccaggtacat tgtgccttca ctaaactcct caactgccag taccacgcca ggacgacgcg
    13501 gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc
    13561 tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caggcgcgcg cgagtgctga
    13621 ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac
    13681 agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gagtacagcg ataccgcaaa
    13741 acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag
    13801 cgaagttcat cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca
    13861 tacatggcgt gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga
    13921 agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg agggggtcgg gatgaaactt
    13981 gcttaagcaa gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc
    14041 ttatccgcaa tttttttcgc tgacaagaaa atattcgcta tatgaatgat taacgctact
    14101 tatgaataga aatgtgacgc acgtcacccc ttggtggctc tggcgtcagc gggatcgctc
    14161 ccgaaaaaat catctttgat aatttgtgct gttttgagaa tcatcctggt aaggggagta
    14221 ctgcgcgact atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac
    14281 cctgctggac agtcatagtt atgactggca accggcagcc ccctggctca gccgtttacg
    14341 cgaagcaaat gttcccgtca ttctctgtag cagtaaaaca tcagcggaaa tgctgtactt
    14401 gcaaaaaatg ttggggctac aaggtttacc gctgattgca gagaatggcg cagtgatcca
    14461 gcttgctgaa caatggcagg agatagacgg ttttccacgc atcatctcag gtattagcca
    14521 tggcgaaatc agccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac
    14581 ttttgatgat gtcgacgatg caaccatcgc cgaatggacg ggattaagcc gtagccaggc
    14641 ggcgctgacg cagcttcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg
    14701 tatggcacaa tttaccgctc gtctgaacga actgggctta cagtttatgc aaggtgcgcg
    14761 cttctggcac gtcctggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc
    14821 gacctatcaa caattgtcag gcaaacgccc aaccacactt ggcctgggcg atgggccaaa
    14881 cgatgcgccc ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga
    14941 aggggtgcat ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc
    15001 ggaaggatgg cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc
    15061 gaatacccga ttgcgcccag cctgtttagc gagataaagc cgacggtcgg ccagtgactg
    15121 gagttgttca aaatcataat caccggtttc ctcgctgcta cttaccccca acgaggcact
    15181 gatgcgtatc gtcgtactct tagcaatcaa catctctttt tcatttaact tcaggcgaat
    15241 acgttctgcg acttccgcag cctccgtcag actcgcgcct ggcagaatca cacaaaactc
    15301 ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag gaactgctaa ttaatccggc
    15361 agcatgagaa agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa
    15421 atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttcgc
    15481 gagcggacgg gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg
    15541 ccacgcctgc cactgcaacg agctttgcag aacatacatg ttgctgacca tccggcgaat
    15601 cacataccag gagatgagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat
    15661 gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttaac gtatggacgc gcaccagcac
    15721 accgtcgaaa tggtccagac gttcccagct aacatagcga ctgtccatac gaatgccgcc
    15781 ccgcgtgtca tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt
    15841 ccctgttgga tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg
    15901 atactcacca tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tagtacgcac
    15961 gggaatactc atccccagca cgccatacca gtagttatta ctgtctaccg gaacactgac
    16021 ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc
    16081 gcggtgacga ttttctcgtt gtgaatggcc gataaaccag ggttgggtga catagccgta
    16141 ataacgcgtc gggacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccggc
    16201 acgcgagaca tacatcgcct gttctaccat cgacgaggag ttgtgtgcca gtcgcagcaa
    16261 gtaaccaact tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag
    16321 gagattcccc tcgctgacta atgcatccga tacgccattg actgacaggg tgcgtcgtcg
    16381 gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacagcatc
    16441 acgcagagag gtgaaatcca gtggcgctac gagagcttcg cgcatgccat tacgcaaaaa
    16501 gatcagtttg tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag
    16561 atgattacgc tggctggaga tataggcatc ttccagcacc actacttcac gccaggtcag
    16621 caaggtggaa aaaagcaata ccacgataaa gcagagatta acgatatgac caggccctag
    16681 gcggcgtgcg agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct
    16741 gacgactccc cacctgcttt tttatgattc tggcataacc tggcgatagc gtaccgcaaa
    16801 acagcgataa ttttataatg gtggcatgat aagacgcgac tatcattcat tgcaggcatt
    16861 ggcacaccag gccggatgtg atccggcctg gtcttaatga taccctttca aacttcttcc
    16921 gcagaacgac tcccctcccc tggaacaagc tcggtttcat gaaaagcttc ccgcttgacc
    16981 ccgtaaccgt cataccaacg acactcaacc ataccgctgg agtatccagt gacaatcatc
    17041 cgcgggccgc cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc
    17101 ttttttcgag tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagcctg
    17161 tgcgagtatt aatgtacctg accgcgcatt ttcgctacgg ctttcactcc cagcaccacg
    17221 atgccgccga tgataaatcc aagaatcaga tttaaaacag tcggtaatat catcgccacc
    17281 actgcacttt gctgcccggc gaaatgttca atggcgtgat gcagcggcgc aataccatgt
    17341 accacaatcc cgccgccgac gaggaacatc gccagcgtgc cgacaatcga taacgctttc
    17401 atcagccagg gcgcgataat caataatcct ttacctaatg cctgcatcag cgcgctggat
    17461 ttttccgcca gccaataccc caggtcatca atcttaacga taacccctac cagaccgtag
    17521 acgcccacgg tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc
    17581 gcttcggcca caatccccag cgtgatggcg acgatttccg cagacaatat aaaatcggta
    17641 cgaatcgccc cttttatttt gtccttttca aacttcagcg gatcctgcgc cgccagcttc
    17701 tccagacgct gctggctctg cgccggatct tctttatgtt tacgcgcctc cagcatatgc
    17761 agcactttct ctaccccttc aaagcagaga aacgcgccac cgatcatcaa caatggcgta
    17821 atcgcccacg ggataaacgc actgatgatc agcgccagtg gcaccagaat cactttatta
    17881 atcagcgatc ctttcgccac gccccagacc acgggaagtt cccggttggc ccgcacgcct
    17941 gaaacttgtt gcgcattgag cgataagtca tcccctaata caccggcggt tttcttcgcc
    18001 gccagtttgc ccatcacgga gatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc
    18061 agtaaactac tcccggccaa aagcattccc tctcattttt attgttgaag ggtgtaagta
    18121 tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc aacatttttt taacaatcaa
    18181 gtgcaattat atctctcgcc agcagaatga ttttcacgtt tactatgaac caccttttta
    18241 tttccaccgt gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt
    18301 gcgttgtata tcatttgggg ctcaacctat tttgtcattc ggattggcgt ggaaagctgg
    18361 cctccgttaa tgatggcggg tgttcgattc ctggcagccg gtattttatt gctggcattt
    18421 ttactactgc gcggacacaa actccccccg ctacgtccgc tgctcaatgc cgcgctgatt
    18481 ggcctgttat tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt
    18541 ccttccggca tcgccgccgt agtggttgca accgtgcccc tctttaccct gtgcttcagc
    18601 cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg gtattgccat tgggcttgcc
    18661 ggaatcatca tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg
    18721 attttaatcg gctcgattag ctgggcgttt ggctcagttt atggctcgcg cattacctta
    18781 cctgtaggga tgatggcggg tgcgattgag atgctggcgg caggcgtggt gttaatgatc
    18841 gcgtcgatga ttgcgggtga aaaactgacg gcgctccctt ccctttcagg cttccttgcg
    18901 gtcggctatc tggcgctgtt tggttcgatt atcgccatca acgcttatat gtatttaatc
    18961 cgtaatgtca gtccggctct cgccaccagc tacgcttacg ttaacccggt ggtcgcggtc
    19021 ttgctgggta cgggactggg tggagaaaca ctgtcgaaga ttgaatggct ggcgctcggc
    19081 gtaattgtct tcgcggtggt actggtcacg ttgggaaaat atctctttcc ggcaaaaccc
    19141 gtagttgctc cagttattca ggacgcatca agcgagtaaa tgtatcccct gcgtgtcgat
    19201 ctgcgcgctg gctccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgcttcatc
    19261 cttcagcttc tcgcgcccac gtaacgcgca ctcccagaca atcaatacgc gccagccgag
    19321 ttcctgcaag cgactgatat cgcggcgatc gcgctcaaca tttttaccta tcttctccag
    19381 ccagaactcg gttcgagtcg caggcacttt aaacagatag cagtgatgat gatgccagaa
    19441 gcagccatgg gtaaatatca cgcagcgata ttcatcaacg acaaaatccg ggcgtccggg
    19501 cagactggcg tcctgaacgc gaaacgccag gccctgcccg gttaacagac tggcgaggcg
    19561 cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gagtggcctt
    19621 atcgtgaacg tcggccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt
    19681 ttggctcaag cagttttgcc accgcggcaa agaccggcac gaccaccgag ttaccgaact
    19741 ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc ggcgcttcaa
    19801 aacccattaa gcgcgcgcat tcccgaggcg ttaaccgacg tggacgatgt tgctgattca
    19861 gcggatcgtc aaagtctttc tcacccgtgg ccatatccca gccgcgatcg attaaaattt
    19921 ccgcgccatc tttgtaataa cgcgcagaca gcgtacgggt gacgctttgc ggattgttcg
    19981 gataaaccat tccataaccg aagccgtttc cgcgcgcctg atgttttttc gcatatcgat
    20041 agaggtactt ccacagcacc ggcgtcagga tatatttcgc ctcgaccatc gggtccaaca
    20101 gctgcgccag cgtcactcgc tgtgcaggga aacattcgct gatatcacgc agagtaaaat
    20161 cggctttcag attaagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg
    20221 gcagaaaatg tttgccatcg atgattttcg gatcgtccgg cccgttatct tctgcatcag
    20281 ccacgtcata gcccagttcg tccagcgtct gcatgatgat gcggaacgtt ttaccctggt
    20341 cgtggctttt caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga
    20401 taatgcgcac cacatcaaag aacagcgtgc cctgagtatc gcaggcaaaa ccgtgcgccc
    20461 gcccgagcga gttctttttc gatacgccag ccagcgaaaa tggctgacaa gggaaaccgg
    20521 ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg ttccgccgca gcctcatcac
    20581 tcacgccttc tttatggctg agggtgatgt cgcggatatc ttcattaaaa tgatgcgtcg
    20641 ccgggtcgca ataatgattg gctttataag tgcgtaccgc atgtttgttc cattcgctgg
    20701 taaacacgca ctgtccgcca atcgattcaa aaccgcgacg gatgccgcca attccggcaa
    20761 atagatcgat aaagcgaaac gcataatgcg gatgatgtgc cggtggtttg ggtaacaacg
    20821 tttgcagatg ggcgaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg
    20881 cacgttttaa aattgccgcg ctccagtgat tctcacccac accattaagc tgagccacca
    20941 gcgttttaac atcataaatt tgcagcagtt tctccagcat tgcctgtgcg gcattcccgg
    21001 tgctgtatga atcggttact gatatatttt cctgcataga tttaaccggc catctgagat
    21061 ggtcgacagg ttaacataat tacagccatt tagggccacg cgctcagccc gcgggtgaaa
    21121 acgcatctat caccttgtga tcgacacctt cattctcccc cgccagttcg gcactgagct
    21181 tcgccataaa ctcgactaaa aaatgcgcgt tgtgctgcgc cagctgcttg cctcttacgg
    21241 tttgcatgat ttgcggtagc ttcaacaact tagtctggaa atgatccagc gcgtagcgtt
    21301 tatcatcaag cgggcgatgc tgtgcgagcg gatcttcggc atcaaacagc gccacgccca
    21361 atgcccctga aacggcaaac acgcgcgcca ggccaatcgc ccccagggct tccagccgat
    21421 ccgcatcctg gacgatttta gcttccgttg ttaagggggc gatttgcgcg ctgaaactgt
    21481 gagcggcaat ggcatgacaa acggcctcga ttttctccgc cggaaattgc acgaactctt
    21541 cacggagcag gcgacgcgtc tcttctgccg ccaggattga agaactctgc cgttgcggat
    21601 gatttttcgc caggctcaca atatcgtgga aataacaggc ggttaaaatc accagcatat
    21661 cgacgtcatc atctgccgcc agcttctgtg ccgtagccca gacgcggcga aaatgacaca
    21721 cgtcatgggc cgcgtcctgg tgctgatgat ggtttttcaa ccagttttcg aactgtgcct
    21781 gccagtgttg taagtccata cgcgctccgt tgccaaagag tcgccacctt agcagttttt
    21841 attcatagcg tctgctacgt gcgggacgag gtcgtgtgta ccaggcaatg ctgcctaaca
    21901 atgccccgac cagagccata aataaaaatc cgaccagtgc cctaaccact ttcccgcggt
    21961 agagcctaaa tcgctttcaa atcccgacac gggggcatcg gggatccggc tcatcaccca
    22021 gatgtaacgc gccattgccc agacgaaata gccacaccat gtataaaaag cccacaaagc
    22081 cagtttgccg ccagggctgc gagagagtct ctcttccatc tttgtcgaac cgtattccat
    22141 tttgagataa cccggaaaat tcagacttag tactattacg tgataaaagt cacatttttc
    22201 cacattggat gagccagtca agactcactc attttttgaa caaatagata atttttgcgc
    22261 aacggcagtg aatacaggaa aaatcgtttt ga
//
LOCUS       DAZFTN010000077        22256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFTN010000077 DAZFTN010000000
VERSION     DAZFTN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..873
                     /gene="yibB"
                     /locus_tag="ACHKV2_003282"
     CDS             16..873
                     /gene="yibB"
                     /locus_tag="ACHKV2_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO6941114.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            1148..2344
                     /gene="kbl"
                     /locus_tag="ACHKV2_003283"
     CDS             1148..2344
                     /gene="kbl"
                     /locus_tag="ACHKV2_003283"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO6941115.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2354..3379
                     /gene="tdh"
                     /locus_tag="ACHKV2_003284"
     CDS             2354..3379
                     /gene="tdh"
                     /locus_tag="ACHKV2_003284"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO6941116.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            3618..4653
                     /gene="waaH"
                     /locus_tag="ACHKV2_003285"
                     /pseudo
     CDS             3618..4653
                     /gene="waaH"
                     /locus_tag="ACHKV2_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(4640..5599)
                     /gene="yibQ"
                     /locus_tag="ACHKV2_003286"
     CDS             complement(4640..5599)
                     /gene="yibQ"
                     /locus_tag="ACHKV2_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO6941117.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(5603..6862)
                     /gene="envC"
                     /locus_tag="ACHKV2_003287"
     CDS             complement(5603..6862)
                     /gene="envC"
                     /locus_tag="ACHKV2_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO6941118.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(6896..8440)
                     /gene="gpmM"
                     /locus_tag="ACHKV2_003288"
                     /gene_synonym="pgmI"
     CDS             complement(6896..8440)
                     /gene="gpmM"
                     /locus_tag="ACHKV2_003288"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO6941119.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            8684..9115
                     /gene="yibN"
                     /locus_tag="ACHKV2_003289"
     CDS             8684..9115
                     /gene="yibN"
                     /locus_tag="ACHKV2_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6941120.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            9257..9508
                     /gene="grxC"
                     /locus_tag="ACHKV2_003290"
     CDS             9257..9508
                     /gene="grxC"
                     /locus_tag="ACHKV2_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO6941121.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            9571..10038
                     /gene="secB"
                     /locus_tag="ACHKV2_003291"
     CDS             9571..10038
                     /gene="secB"
                     /locus_tag="ACHKV2_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO6941122.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            10038..11057
                     /gene="gpsA"
                     /locus_tag="ACHKV2_003292"
     CDS             10038..11057
                     /gene="gpsA"
                     /locus_tag="ACHKV2_003292"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO6941123.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            11137..11958
                     /gene="cysE"
                     /locus_tag="ACHKV2_003293"
     CDS             11137..11958
                     /gene="cysE"
                     /locus_tag="ACHKV2_003293"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO6941124.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(12011..12484)
                     /gene="trmL"
                     /locus_tag="ACHKV2_003294"
     CDS             complement(12011..12484)
                     /gene="trmL"
                     /locus_tag="ACHKV2_003294"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO6941125.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(12670..13860)
                     /gene="lldD"
                     /locus_tag="ACHKV2_003295"
     CDS             complement(12670..13860)
                     /gene="lldD"
                     /locus_tag="ACHKV2_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO6941126.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            complement(13857..14633)
                     /gene="lldR"
                     /locus_tag="ACHKV2_003296"
     CDS             complement(13857..14633)
                     /gene="lldR"
                     /locus_tag="ACHKV2_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO6941127.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            complement(14633..16288)
                     /gene="lldP"
                     /locus_tag="ACHKV2_003297"
     CDS             complement(14633..16288)
                     /gene="lldP"
                     /locus_tag="ACHKV2_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6941128.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            complement(16656..21422)
                     /gene="ehaG"
                     /locus_tag="ACHKV2_003298"
     CDS             complement(16656..21422)
                     /gene="ehaG"
                     /locus_tag="ACHKV2_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO6941129.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            complement(21466..22134)
                     /locus_tag="ACHKV2_003299"
     CDS             complement(21466..22134)
                     /locus_tag="ACHKV2_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6941130.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
BASE COUNT         5220 a         5621 c         5742 g         5673 t
ORIGIN      
        1 atggtcttat tagtaatgaa atcatccacc acaattatca ctgcatattt tgacattggt
       61 agaggggatt ggactgctaa taaagggttc cgtgaaaaac tagctcgttc agttgatgtt
      121 tattttagtt actttgagcg tctggccgca cttgaaaatg agatgattat tttcacttct
      181 cccgatttga aaccaagggt tgaagcgatt cgcaacggaa aaccaacaac ggttatcgtt
      241 atcgatataa aaaagaaatt tagatatatc agaagtcgta tcgaaaaaat tcaaaaagat
      301 gagtcgttta caaatagact tgagcctcga caattaaaaa acccagagta ctggtcacca
      361 gagtatgttt tggtatgtaa ccttaaggcc tattttgtaa ataaagctat caacatgggc
      421 ttagtgaaaa cgcctcttgt tgcgtggata gattttggct attgccgtaa gccaaatgta
      481 actcgaggat tgaaaatttg ggatttccca tttgatgaaa gtaagatgca tcttttcact
      541 ataaaaaaag ggcttactgt cacatctcaa caacaggttt ttgatttcat gattggcaat
      601 catgtatata ttattggggg cgcaattgtt ggttcacagc ataaatggaa agaattctat
      661 aaattagttc tggaaagtca aaaaataaca ctaaataata acattgtgga tgatgaccag
      721 ggcatttttg tcatgtgcta ttacaaacgc cccgatctct tcaacctaaa ctatctgggg
      781 agaggaaaat ggttcgattt gtttcgctgc ttcaggagta acactttagg ggcaaaaatg
      841 caggcgctga gaattttcct ttcaagaaaa taggtttccc tccattagtg ggtagcgttt
      901 tgataccaac tgagatagga aaaatgatgc tttggcatca ttttttcttt ttatatcaat
      961 gcagtaaaat caatcatcat tatgcccagc caaaactgta cagtttttct actatctgta
     1021 ggtgttgaga gacatcaagt tacacgtcat ttatcctgaa ttttgcagaa gagttaacgc
     1081 gtcatctcgt cgcgacctat aagtttgggt aatatgtgct ggaatttgcc ctgtctggag
     1141 aatcgcaatg cgtggagaat tttatcagca gttaaccaac gatctggaaa ccgcacgggc
     1201 ggaagggttg tttaaagaag agcgcattat tacgtctgcg cagcaagcag atatcactgt
     1261 ggctgatgga agccacgtca ttaacttttg tgccaacaac tatctcgggc tggcgaatca
     1321 tccggatctg attgcggcag caaaggcggg aatggattct cacggtttcg gcatggcttc
     1381 ggtgcgtttt atttgcggca ctcaggacag ccataaagag cttgaacaaa aactggcggc
     1441 attcctgggg atggaagatg cgattctcta ctcttcctgc tttgatgcta acggtggact
     1501 gtttgaaacg ctgctgggtg cggaagacgc cattatctcc gacgcactga accacgcgtc
     1561 tattattgat ggtgtgcgtc tgtgcaaagc taaacgctat cgctatgcca acaacgatat
     1621 gcaggagctg gaagcacgtc tgaaagaagc gcgtgaagcc ggtgcgcgtc atgtgctgat
     1681 tgccaccgat ggtgtgttct caatggacgg cgtgattgcc aacctgaaag gcgtttgcga
     1741 tctggcagat aaatacgatg ccctggtgat ggtggatgac tcccacgcgg tcggttttgt
     1801 cggtgaaaat ggtcgtggtt cccatgaata ctgcgatgtg atgggccggg tcgatattat
     1861 caccggtacg cttggtaaag cgctgggcgg ggcttctggt ggttataccg cggcgcgcaa
     1921 agaggtggtt gagtggctgc gccagcgttc tcgtccgtac ctgttctcca actcgctggc
     1981 accggccatt gttgccgcgt ccatcaaagt actggagatg gtcgaagcgg gcagcgaact
     2041 gcgtgaccgt ctgtgggcga acgcgcgcca gttccgtgag caaatgtcgg cggcgggctt
     2101 taccctggcg ggagccgatc atgccattat tccggtcatg cttggcgatg cagtagtggc
     2161 gcagaaattt gcccgtgagc tgcaaaaaga gggcatttac gtcaccggtt tcttctatcc
     2221 ggtcgttccg aaaggtcagg cgcgtattcg tacccagatg tctgcggcgc atacccctga
     2281 gcaaattacg cgtgcagtag aagcattcac gcgtattggt aaacaactgg gcgttatcgc
     2341 ctgaggatgt gagatgaaag cgttatccaa actgaaagcg gaagagggca tctggatgac
     2401 cgacgttcct gtaccggaac tcgggcataa cgatctgctg attaaaatcc gtaaaacagc
     2461 catctgcggg actgacgttc acatctataa ctgggatgag tggtcgcaaa aaaccatccc
     2521 ggtgccgatg gtcgtaggcc atgaatatgt cggtgaagtg gtaggtattg gtcaggaagt
     2581 gaaaggcttc aaaatcggcg atcgcgtttc tggcgaaggc catatcacct gcggtcactg
     2641 tcgcaactgt cgtggcggtc gtacccatct gtgccgcaac accattggtg tcggtgttaa
     2701 ccgtccgggc tgcttcgccg aatatctggt gattccggcg ttcaacgctt tcaaaatccc
     2761 cgacaatatc tccgacgacc tggcttccat ttttgatccc ttcggtaacg ccgtgcatac
     2821 cgcgctgtcg ttcgatctgg tgggcgaaga tgtgctggtt tctggtgcag gtccgatagg
     2881 tattatggct gcggcggtgg cgaaacacgt tggtgcacgc aatgtggtga tcaccgatgt
     2941 taacgaatac cgccttgagc tggcgcgcaa aatgggtatc acccgtgcgg ttaacgtcgc
     3001 gaaagaaaac cttaatgatg tgatggctga actgggcatg accgaaggct ttgatgtcgg
     3061 tctggaaatg tccggcgcgc cgccagcgtt tcgtaccatg ctcgatacca tgaaccacgg
     3121 cggtcgtatt gcgatgctgg gtattccacc gtctgacatg tctatcgact ggaccaaagt
     3181 gatctttaaa ggcttgttca ttaaaggcat ttacggtcgt gagatgtttg aaacctggta
     3241 caagatggcg gcgctgattc agtctggcct cgatctctcg ccgatcatta cccatcgttt
     3301 ctctatcgat gatttccaga agggctttga cgctatgtgt tcgggccagt ccgggaaagt
     3361 tattctgagc tgggattaac acgaaaaagg gctggtattc cagccctttt gcctgaggat
     3421 aatctgttaa atatgtaaaa tcctgtcagt gtaataaaga gttcgtaatt gcgctgatct
     3481 tttatatagc tgttctcatt atctctctac cctgaagtga ctctctcacc tgtaaaaata
     3541 atatctcaca ggcttaatag tttcttaata caaagcctgt aaaacgtcag gataacttca
     3601 gaggtcgtcg gtaatttatg atgaacagca ccaataaact tagtgttatt attccgttat
     3661 ataatgcggg cgatgatttc cgcacttgta tggaatcttt aattacgcaa acctggactg
     3721 ctctggaaat cattattatt aacgatggtt caacggataa ttctgttgaa atagcaaagc
     3781 attacgcaga aaactatccg cacgttcgtt tgttgcatca ggcgaatgct ggcgcatcgg
     3841 tagcgcgtaa tcgtgggatc gaggtggcga cgggcaaata tgtcgctttt gtcgatgctg
     3901 acgatgaggt ctatcccacc atgtacgaaa cgctgatgac catggcgtta gaggacgacc
     3961 tcgacgtggc gcagtgcaac gctgactggt cttttcgtga aacgggagaa acctggcaat
     4021 ccatccccag cgatcgcctt cgctcaaccg gtgtattaac cggcccggac tggctgcgga
     4081 tggggctttc ttcgcgccgt tggactcacg ttgtctggat gggggtttat cgccgtgatg
     4141 ttattgttaa aaataacatt aaatttattg ccggattaca tcatcaggat attgtctgga
     4201 caacagaatt catgtttaac gcgctgcgtg cgcgatatac cgagcaatca ttatataaat
     4261 attatctgca taatacgtca gtgagtcggt tgcatagaca agggaataaa aaccttaatt
     4321 atcaacgtca ctatattaag attacccgcc tgctggagaa attaaatcga aattatgccg
     4381 acaaaattat gatttatccg gaatttcatc agcaaataac ttacgaagca ttgcgtgttt
     4441 gccatgcggt gcgcaaagag ccggatattc ttacccgcca acggatgatt gccgagatat
     4501 ttacttccgg tatgtataag cgcctgatta ccaatgtgcg cagcgtgaag gtcggttacc
     4561 aggcgttact gtggtctttc cgcttatggc aatggcgcga aaaaaacgcg gtcgcaccat
     4621 cgcattacgc gtagcgcctt taatttgcgc tagcgttaaa cttcgccgct tcgggctgtt
     4681 tgccccagcc ttgccactga tgctggaagt aaacgatcag cgtgctctgg ctgatgcttt
     4741 cgcttaatac ttcaaagaag cgattagcat aaacaggttc tatcggtttc ttcggtttgc
     4801 acagcttcac gccacggaac ggattacgcg gtgcatcagg cacagcgttt ttcggcggtg
     4861 tagaagtatc aacctgcggt tcattcagca agctgctggc tttcaccagc gtaatgtctg
     4921 gcggcaggtt ataaaccatc tgttgtagca cgcgtaccgt tgaagggtga ggatgcccaa
     4981 tggcaatggt tgaaccgttg cgacgcgcca gatcaattgc gcgattaaat tgcacacgga
     5041 tgtccgcttc gttctgcgaa tcgtcgagga acaccttccg tttgatcacc ttcacgccag
     5101 taccttgcgc ggcgcgcatc gcctgggtat taccgatggt tacgctgtcg aggaagtaaa
     5161 gattgtaacg ctccagtgcc tgcatcactt tctgcatacc aaacaggtta gaggtcatct
     5221 tgctacccat gtggttgttg atccccacgg catagggcac gttattgacc gcactacgaa
     5281 taatgcgctc aatttcgtcg ctgctcatct ccgggcgcag cgtatttttc tccagcggct
     5341 gtttactcag tggtgccatc gggagatgaa tcaacacttc gtgcccgctg ttatgcgctt
     5401 tggtcgccat ctctctggcg tgcggtgaat cgggtaatac agcgacggag atagcggagg
     5461 gcatcgccag cacctggttt tcgttgtgcg ggcgataccc aaaatcatca atgacgatgg
     5521 caagtttgcc agcaagtacg ggggaggaga gcgccaacag agcggcaaat gcaagaacgt
     5581 tacgacgaaa tggaaacaaa acttatcttc ccaaccacgg ctgtggattg accgcctgac
     5641 cttggcggcg aatttcgaaa tagagtgaag gccgaccctg accgccactg ctgcccacca
     5701 gtgcaattgg ctggcccgcg cgaacctgcg aaccaacgct caccagtgcg ctctgattat
     5761 agccgtaaag actcatgtcg cctttaccgt gctcaaccac caccaccaga ccgtaacctt
     5821 gcagccagtc agccagaatc acccgaccat cggcaatcgc tttaacttca gtaccttcag
     5881 aggcaccgat aaccattcct ttccagcgta gttcgccctg tagctgttca ccatagcgat
     5941 gcagcgtcgg cccgcgaacc ggccagaatg cctgaccacg cggcgccccc aggccaccag
     6001 ttcgggacat cagcgatttt tcgctttcgg tcggtttgta ggtggtgcct ttgcgcgtcg
     6061 cttctttctg gcggtcgcga accgcctggg cctcacgtgc ttctcgttct gcacgcgctt
     6121 tcgccgcggc ttccgcacgg gcaatgctgt tacgcagacg ggattcgttg gcgcgcagct
     6181 cgctcaactg ttgctgacct tgctggatgg aagactccag ccctgccagc gtctttttac
     6241 gctcgttcag cgcctgagtc agtttcgcct gttgggcgcg ctgctcatat aacagcgttt
     6301 gttgctcgct ctgtttctct tccagttcag cacgctgcat ggcgacttct tcacgcgttt
     6361 gcttcaactg ggcaatggtt tcttgtcgcg cctggttgag atagccgaaa taagcctgta
     6421 aacgctgtcc acgctggctt tcttcaccgc tgagaatcag ctgaataccg gtatgctcgc
     6481 cctgacggaa tgcggcatcc agttgtgcgg cgaggctgcg ctcctgggcg gctttttgct
     6541 gctccagttt ggcaatcgac gcgttcatct catcaatctg tttattgagt tgattgagcg
     6601 tgttttgcgt ttcgcgcagc ttacgggtgg cttcagagat cgcttcttcc tgctttttca
     6661 attgtgcgag caggcttgcg cgttgttgtt gcttttggcg taccgcgcgc tcttttgcgg
     6721 cgatatcggc ctgaatagat ttgagttggt cacgctcatc cgcgtgggcg gaaaaggcgc
     6781 acaacaatac gccagcgcta agaacgctgg cgtagatgat gggcctgatt gcaaacctgc
     6841 gcggtttcac ggcccgtgtc atggtattaa tcgcctttcc cctcatgggg agggattatt
     6901 ccacgatgaa cagcggctta ccagtcatct cttgcgggat ttccataccc atcagcgaca
     6961 acatggtcgg cgcgatgtca gaaagtttgc cgcctgcaac cgctttcacg ttcttatcac
     7021 caacgtaaat cagcggaact ggcaggttgg tgtgtgccgt gtgtgcctga cccgttgccg
     7081 gatcgcgcat ctgttcagcg ttaccgtggt cagcggtgat cagcagttgt ccacccacgg
     7141 actcaaccgc tttcgcgact tcttccacgc agtgatccag tgcttcaacc gctttaaccg
     7201 ccgcttccat caccccggtg tgacctacca tgtcgccgtt cggatagtta cagataatgg
     7261 tgtcgtattt gccgctcttg atggccgcaa ccagtttttc ggtcagctct gcggagctca
     7321 tttccggttg cagatcgtag gtagccactt tcggtgagtt gatcagaatg cgatcttcgc
     7381 ctttgaacga ctcttctacg ccaccgttga agaagaaagt aacgtgggca tatttttcgg
     7441 tttcggaaat acgcaactga gttttgtcgt ttttcgccat ccactcgccg aaggtgttaa
     7501 ccagggaagc gggtgggtaa gcaaccgcag ttttgatgtc agcggcgtat tcggtcagca
     7561 tcacgaaatc gacgttaacc actttcttac gcgcgaagcc gtcgaaatct gcgtttacga
     7621 atgcacgagt gatttcgcgc gcgcggtcag cacggaagtt catgaaaatc agcgcatcgc
     7681 cgtcttccat tgccgcatct ggctgacctt ctgcacgaat aacggtcgct ttcacgaact
     7741 cgtcgttttc gtcgcgagca taagctgcct gcaaaccagc aacggcagta tcggcctgga
     7801 actcaccctg cgccagagtc agcaggtcat aggctttttc aacgcgatcc caacggttat
     7861 cgcggtccat cgcgtagtaa cgaccaatga tggacgctac gcggccttta cccagcgcag
     7921 caaatttctc ttcaaatttt ttcagcgagg attcagcact gcgcggcggg gtgtcgcgac
     7981 cgtcaaggaa tgcgtgcagg tagatttttt ctgcgccgcg ttcagctgcc agttctacca
     8041 tcgccatgat gtgatcttcg tggctgtgta cgccgcctgc ggagagcaga cccataatgt
     8101 gtactgcttt acctgcgttt ttcgctttat ctaccgcgcc agtcagcact ggattagcaa
     8161 agaaagcgcg atctttgatt tcaacgtcca gacgagtcag gtcctgatac acgatgcggc
     8221 cggcacccag gttaacgtgg cctacttcgg agttacccat ctgacggtca ggcagaccga
     8281 cttccagacc ggaagcgtcg atcagggtat gcggacgatt ggcccacagt gcatccatta
     8341 ccggggtttt agcactaaaa atggcgttat cctgctgttc ttcacgatag ccatagccat
     8401 ccagaatcac cagtaccata ggttttttag aaaccgacat tgcgacaacc tcatactcaa
     8461 gagtcaaaat ttgcgtaatt ttactacagc tgaatcgata aaatagcctc tgaagatcaa
     8521 aatatacgac cgctcgcggc aagccgatgc cagatttgcg ctttttttcg tcgtgccccg
     8581 caaaattgca ttccagttaa cgcgctggct gtatttgccg caccgcgcag gtatactcct
     8641 ttcctggttt ttttaatcac tacgtcggga gttgttaccc cccatgcaag aaattatgca
     8701 atttgttggc cgtcatccca tactgagtat cgcctggatc gcgttactgg tggcggttct
     8761 tgtgactacg tttaagagcc tgacctcgaa agtgaaggtg attactcgtg gtgaagctac
     8821 gcgtctgatc aacaaagaag acgctgtggt tgtggattta cgtcagcgtg atgacttccg
     8881 taaaggccat atcgcaggtt ctatcaatct gttgccgagc gaaatcaaag ccaacaatgt
     8941 tggtgagctt gagaagcaca aagacaaacc ggttatcgtg gtagacggtt ctggcatgca
     9001 gtgccaggag cctgcaaacg cactgacgaa agctggtttt gcgcaagtat tcgtgctgaa
     9061 agaaggcgtc gctggctggg ctggcgaaaa cttgcctttg gtgcgcggca aataatttac
     9121 ctgtcgtctt tcatgatgca ggccgttagc tgcaattagg cccccagcga cttattgtta
     9181 taagcttctg gggacactcc attttgcagc cgccctgcaa catgaaattt cagggtaaaa
     9241 aaacaggagc taaattatgg ccaatgttga aatctatacc aaagaaacct gcccgtattg
     9301 ccatcgtgca aaagcactgc tgagcagcaa gggcgtgagt tttcaggagt tgccgatcga
     9361 tggcaatgcc gccaagcgtg aagagatgat caaacgcagc ggtcgcacca cggttcccca
     9421 gatttttatt gacgcacagc acattggcgg ctgtgatgac ttgtatgcat tggatgcacg
     9481 tggtggactg gatcccctgc tgaaataacg tgtgaacgtt ggcattacat tgcgcagtat
     9541 ttaaggacaa cacttaaggg ttttctacac atgtcagaac aaaacaacac tgaaatgact
     9601 ttccagatcc aacgtattta taccaaggat atctctttcg aagcgccgaa cgcgccgcac
     9661 gttttccaga aagattggca accagaagtt aaacttgatc tggatacggc atcttcccaa
     9721 ctggcagatg acgtatacga agtggtactg cgtgttaccg taacggcctc cctgggcgaa
     9781 gaaaccgcgt tcctgtgtga agttcagcag ggcggtattt tctccatcgc gggtatcgaa
     9841 ggcacccaga tggcacattg cctgggcgca tactgcccga acattctgtt cccgtatgct
     9901 cgtgagtgca tcaccagcat ggtatcccgc ggtacattcc cgcaactgaa ccttgcgccg
     9961 gttaacttcg atgcgctgtt catgaactat ttgcagcagc aggctggcga aggtactgaa
    10021 gaacatcagg atgcctgatg aaccaacgta atgcttcaat gactgtgatc ggtgccggct
    10081 cgtacggcac cgctcttgcc atcaccctgg caagaaatgg ccacgaggtt gtcctctggg
    10141 gccatgaccc tgaacatatc gcaacgcttg aacgcgaccg ctgtaacgcc gcgtttctcc
    10201 ccgatgtgcc ttttcccgat acgctccatc ttgaaagcga tctcgccact gcgctggcag
    10261 ccagccgtaa tattctcgtc gtcgtaccca gccatgtctt tggtgaagtg ctgcgccaga
    10321 ttaaaccgct gatgcgtcct gatgcgcgtc tggtgtgggc gaccaaaggg ctggaagcgg
    10381 aaaccggacg tctgttacag gacgtggcgc gtgaggcctt aggcgatcaa attccgctgg
    10441 cggttatctc tggcccaacg tttgcgaaag aactggcggc aggtttaccg acagctattt
    10501 cgctggcctc gaccgatcag acctttgccg atgatctcca gcagctgctg cactgcggca
    10561 aaagtttccg cgtttacagc aatccggatt tcattggcgt gcagcttggc ggtgcggtga
    10621 aaaacgtcat tgccattggc gcggggatgt ccgacggtat cggttttggt gcgaatgcgc
    10681 gtacggcact gatcacccgt gggctggctg aaatgtcgcg tcttggcgcg gcgctgggtg
    10741 ccgatcctgc cacctttatg ggcatggcgg ggctgggcga tcttgtgctt acttgtaccg
    10801 acaaccagtc gcgtaaccgc cgttttggca tgatgctcgg tcagggcatg gatgtacaaa
    10861 gcgcgcagga gaagattggt caggtggtgg aaggctaccg caatacgaaa gaagtccgcg
    10921 aactggcgca tcgcttcggc gtagaaatgc caataaccga ggaaatttat caagtattat
    10981 attgcggaaa aaacgcgcgc gaggcagcat tgacgttatt aggtcgtgca cgcaaggacg
    11041 agcgcagcag ccactaaccc cagggaacct ttgttaccgc tatgacccgg cccgcgcaga
    11101 acgggccggt cattatctca tcgtgtggag taagcaatgt cgtgtgaaga actggaaatt
    11161 gtctggaaca atattaaagc cgaagccaga acgctggcgg actgtgagcc aatgctggcc
    11221 agtttttacc acgcgacgct actcaagcac gaaaaccttg gtagtgcact gagctacatg
    11281 ctggcgaaca agctgtcatc gccaattatg cctgctattg ctatccgtga agtggtggaa
    11341 gaagcctacg ccgctgaccc ggaaatgatc gcctctgcgg cctgtgatat tcaggcggtg
    11401 cgtacccgcg acccggcagt cgataaatac tcaaccccgt tgttatacct gaagggtttt
    11461 catgccttgc aggcctatcg catcggtcac tggttgtgga atcaggggcg tcgcgcattg
    11521 gcaatctttc tgcaaaacca ggtttctgtg acgttccagg tcgatattca cccggcagca
    11581 aaaattggcc gtggcattat gctcgaccac gccacgggca tcgttgtggg tgaaacggcg
    11641 gtaattgaaa acgacgtatc gattctgcaa tctgtgacgc ttggcggtac gggtaaatct
    11701 ggtggtgacc gtcacccgaa aattcgtgaa ggtgtgatga ttggcgcggg cgcgaaaatc
    11761 ctcggcaata ttgaagttgg tcgcggcgcg aagattggcg caggttccgt ggtgctgcaa
    11821 ccggtgccgc cgcatacaac cgccgctggc gttccggctc gtattgtcgg taaaccagac
    11881 agcgataagc catcaatgga tatggaccag catttcaacg gtattaacca tacatttgag
    11941 tatggggatg ggatctaatg tcctgtgatc gtgccggatg cgatgtaatc atctatccgg
    12001 catacagtaa ctaatctctc aacaacgctc ccggatatcc cagctgccgc caggcttcat
    12061 acaccacaac tgacaccgca ttggacaggt tcatgctgcg gctgtccggc accatcggaa
    12121 tgcgaatttt ttgttcagcg ggcagggcat caagaatgct cgctggcagg ccgcgtgttt
    12181 ccgggccgaa catcagataa tcgccatcct gatagtttac ggcgctgtga gcaggcgtac
    12241 ctttcgtggt gagggcaaac aggcgctggg gattttctgc ttcgaggaac gcgcgaaagt
    12301 catgatgacg cgtaacggcg gtaaactcgt gatagtccag ccccgcgcgg cgcaggcgct
    12361 tatcgtccca ggcaaatccc atcggttcga tgatatgcag acgaaagccg gtattagcgc
    12421 aaagacggat gatattgcca gtatttggcg gaatttctgg ttcgtaaagt acgatgttta
    12481 gcatactgcc cccttagtgc gggggcagga tagcagaaaa ttggtgagca gtgcggcctt
    12541 ttcccctcac cccggagggg cgagggagaa aaaaagagtg cggtgtcgtt tcagagtgag
    12601 ggagtacata cagcgccgaa cggtccccac tccctgaggg agagggttag ggtgaggggg
    12661 ctcaaacgac tatgccgcat tccctttcgc catcggagcc agtgccgtag gcaactcttt
    12721 acccagcccc tgcaccagcg aatcttgcgt aatttcactg atcgatttcg cgccagtcag
    12781 cgtcattgcc actttcatct ctttctcgat cagatttagc aggttagcta cacccgcctg
    12841 gcccgctgtt gccagcgcat acaggaaagc acgacccagc agtacggtgt cggcaccgag
    12901 cgcaatcata cgcacgacat caagcccgtt acgaattccg ctatccgcca gaatggcgat
    12961 atcacctttc accgcatctg caatagcggg caacgcacga gcggaagaga gtacaccgtc
    13021 cagctggcgg ccaccgtggt tagaaactac aatcccatca gcaccaaaac gtactgcatc
    13081 gcgcgcatct tccggatcga ggatcccttt gatcaccatc gggccatccc agaaatcgcg
    13141 gatccactca aggtctttcc atgagatgga cggatcgaag ttattcccca gccagccgat
    13201 gtaatcttcc agtccggtcg gtttgccgag gtaagccgaa atattaccta aatcatgcgg
    13261 acgaccgttc aggcccacat cccacgccca ttgcggatgc gtcaccgctt gcaagtagcg
    13321 gcgcattgcc gcatttgggc cgctcatccc agaatgcgca tcacgataac gcgctcccgg
    13381 tgtcggcata tccacggtga aaaccagcgt cgaacaaccc gctgcttttg ctcgctccag
    13441 cgcgttacgc ataaagccgc gatcgcgcag tacataaagc tggaaccaca ttgggcgctt
    13501 gatggctggc gcgacttctt caatcgggca aacggaaacc gttgagagag taaacggaat
    13561 gccatgcgcg tccgccgctt tggctgcctg aacttcgcca cgacgcgcat acatgccaca
    13621 caaacccacc ggtgccagtg ctaccggcat cgacaatttc tcattaaaca gcgtcgtttc
    13681 caggcttaag tcagacatgt ttttcagaat acgctggcgc agtgccactt ctgacaaatc
    13741 ttccacgttg cggcgcagcg tgtattcaga atatgcacca ccatccatat agtggaacag
    13801 gaacggcggc agaatgcgtt gcgctgcggc gcgataatcg ctggctgcgg aaataatcat
    13861 gcgtttttct ccctcgaatg ctcattatgc tcaccgggca ggcgggtaat ccgtgcgtgg
    13921 cgagcctgat cttcatcgaa tcgtttcatg gtggtgtgaa caaaactaag gtgcgccatc
    13981 attgctttac gcgccccgtc ggcatcacca gcaaaaatgg cgtcaatgac agcctgatgt
    14041 tgttcggtca gttgtgaaaa aaccggtggc accagataca tccgctgacg gctatgcttc
    14101 actgaggatt gcaggacatc gaagaaaccg cgtatggttt gcagcagcac gatgttatgt
    14161 gaggcttcgg caatcgccag atgaaaacga acgtccgctt gtgaggcgat atccgggtct
    14221 tcacttagcg ttgcttcaaa gcaaagctga atcttttctt tgtcgccagg tgtggcgcgc
    14281 attgccgcat gccatgcggt gctggcttca atggcgtagc gggcttccag aatatcgaaa
    14341 ctgtaatccg gatcatcggc catcagcgtt tttagcggct ggacgatgtt ttgctccgac
    14401 catgtgtcat gacgccagcg aataaacgtc ccgccgccgc gtcgactgag cagcacgcct
    14461 tcgctcacca gttttgccag cgcctcgcgt agtgaattac gtgatacgcc gagttgcatc
    14521 gccagttggc gctcagcggg caacttcatg cccgcttcca ggtttttttc atcaatcagc
    14581 gcccgcacac gatcggcaac ctcgtctgac aggcgtctgg gtaaaacaat cattaaggaa
    14641 tcatccacgt taagacataa gcctgaagcg tggtgatcac gcccaccata caggtgaaga
    14701 tcaggctgtg tttgacagta aagcggaaca aatcagactc tttgcccacc aggcctaccg
    14761 ccgcacaggc gatagcgata gattgcgggg agatcatctt accggtgacg ccaccggtgg
    14821 tattggcggc aaccagcaac agatcagaga cgccaatttg ttgtgctgcg gtggcttgca
    14881 gcgcggcgaa cagggcgtta gatgaggtat ccgacccggt caggaatacc cccagccagc
    14941 cgaggaacgg cgagaagaag gtgaatgcat gaccggtgtg cgccagtgcc agtgccagtg
    15001 ttgatgacag tccggaatag ttcgagataa aggcgaacgc cagcaccatg ccgatggagt
    15061 agatgggtag agccagttct ttcagcgtgc tgccgaaggt gctgatagcg tcagacggtt
    15121 tcatcttcag ccagacaatc gagagcagtg cagcaaacag aatggcggtg ccggtggcag
    15181 agaaccagtc aaacttaaac acggcggcat aggctgtagc ctcgctgacc actggcggca
    15241 tacgggcaac cagtttatcg aggtacggca ccggaatgtt gatcacccac tcatacagcg
    15301 cgccacccga tgcgaacagg gctttaaacg gcgggatact ccacagtgtt acggtagctg
    15361 tcaggaacag gaacggtgtc caggcacgta acacctgacc cgcagtgtaa ccggtgtggg
    15421 ctagcgtcat atcaacctgt gacgccccca aatcaccaaa acggaatacg cgcactggct
    15481 gccagcgttt gaggaacagc gtcaggcaga gcagtgatac cagcgaagag ataatgtccg
    15541 gcagctccgg cccaatgaag ttagagctaa ggtactgagc gatggcaaac gagccgcccg
    15601 caaccacgac cgcaggccac gtctccttga taccgcgcca gccgtccata atcgccatga
    15661 tccagaacag cacgataatg gtcataaacg gtagctgccg ccccaccatc tggccaatct
    15721 caaagctgtc gatgcctgtt acttgtccgg caaccaggat tggaatgccc atcgcgccaa
    15781 atgccactgg cgcggtgtta acaatcaggc acagtccggc ggcgtacagc ggtttaaaac
    15841 ccaggccgac cagcaatgcg gcggtaattg ctaccggtgc gccaaagcct gcggctcctt
    15901 caaggaaagc gccgaaacag aaaccgacga tcagcatttg cagacgctgg tcaggggtta
    15961 tcgaaagaat agacgagcga atgatgtcaa actgcccggt tttcaccgag atcttataga
    16021 cgaacactgc tgcaataatg atccacgcga tgggccacaa accatagaag aagccataaa
    16081 ccaccgaggc cagcgcgtta gcgaccggca ttttatagaa cagcaaagcc acggcaaggg
    16141 cgattgctac cgtccatgag gcggcgacgt atcctttcag tttgagctta atcagcgcaa
    16201 agaagaaaaa caggatggga agcgatgcta tcaaactgga aagccagata ttcccggcgg
    16261 gatcgtagtt ttgttgccag agattcattg caggtctcct ggagtccacg cacatcgtgt
    16321 aatgagtctg ttgctcatct ccttgtcaca cgttgtgtaa aagtggcact gccaattgtg
    16381 ttgtgtaggg ataatgacaa tgaatggtta actaaatgtt atgtattggc aatattgatg
    16441 tgagttttaa ttggggaatt gtgaaccaga ggtcagataa gcgaagaatt ggtagggcca
    16501 attcttgtgg ggggagatgt ctggtaaaga aaagggcaat cctcatcaga gaattgccct
    16561 tagattgttg acccggagcg ccttatcttg cctgatgcgc gtaacgcatc aggcaatttg
    16621 gcgtttgccc ttagtcacac taacttaatg gatcattacc actgaatacc ggcaccgagt
    16681 gcggcggagt attcaccctg gctattggta ctaccttgta atttgtagac ccaacgacca
    16741 ttggcgctca ccatcgatac acctaaagca actgccgatt caccgttgta agtaccgcca
    16801 ccaatagagg ccatgctggc acctggagtg taagcctgcg gcagaccggt cattgccatt
    16861 gcagaagcga taccaccgct caacttgctt tcagttttag acagtttgtt atccatctca
    16921 accattcgct gatcggtgta ttgcttcgtt tcctgcacgc tttgcttcaa ctgcgcgtaa
    16981 ttcaccacgt cgttgttgtt gacgccagcg gagacgttgc tgatacgagt cgtaccgccg
    17041 ttgccgccac cgagggtgat attgctatag tcgatagaac catcagcttt ggtgtcgtaa
    17101 cgtacaccgc cagcttcgga agacttcaac tgtgccacgt taacagcatc ggtagcattt
    17161 ttacctgcag ctacgttggt gatacgacgt tggttagtag aggaacctac agagatcgta
    17221 ttttcttcgg ttgccacaga ccctgtaccc agagcgacgc tgttgtcagc ggcagcaatg
    17281 gagccggaac caatcgcgac gctatcttta ccctgcgcgc tggcatctac accatcggta
    17341 ttggtcttga agtacttggt gctaccggtg gtgacgatat cgccaatacc gttttcgata
    17401 ttggtgacgc gtgaatcaag attcgttacc cgattatcca ggttagtaat gctctgggta
    17461 ttctgggaaa cctgcgcatc cgttactttc aactgaccca cgttgaccgc atcggtatca
    17521 gccgaacctg ctgcgacgtt agtgatctga cgttgaccat ccgcactacc gactgagaat
    17581 tcaccgacag agttctgcgg tgcgtccatg ttgtaggcgg tataattggt ttgagcgcca
    17641 cgagtggtcg tagaaccatt acctatcgca atactgttga catgaatgac ttgcgcattg
    17701 ctaccaatgg caatgccgtt aagtgcattc gcgtccacgt aggcaccata accgatacca
    17761 atacctttat cgccattcac gatggttttc gcacccattg ccagcgagtc agttcccact
    17821 gccagtgaat cttcttccgt tgaattagcg tggaagtatt tagtcggcgt ggttgcgacc
    17881 gcaccaatcg cattctgcaa ttgacgaacc gtaacggcgt catgggcttc ggaaccatcg
    17941 gctacgttga tgatttgacg atatttaccg tcatcaccga tagacaatgc accgagcagt
    18001 tcgccatccg tggtgtcgta accaataaca acgccatttt ccgttatgct ggtgtcacgg
    18061 gagcctttgg caatcactcg gctggaaaca gagctgctac ccagggcgat acccgtatca
    18121 acgtcgctgt agctgccctg accaatagct acgctgctat cgcctttggc gacagagtta
    18181 taacctatag ctgtagcgcc aacaccctgt gcgctggcgt cgttgaacgc taagccgtcg
    18241 tcgttagtac gcacatagtt aatacccgca ccgttttctt ggatataggt tgcgtcggtg
    18301 ttaccagcga gctgattgat aatttgggtg ttctgctcaa tcatcatatt cgtcgcattc
    18361 aactgagaac cgttcactgc atcggtactg tcctcactaa tactgccatt agcgacatta
    18421 gtgatgatgc tggctttacc gtcatggctg gcattgtagg ctccagcacc gccattggca
    18481 gtctcatccc acagcagggc gttatcatca agcgcatcca gggcgtcacc gacgttgttg
    18541 tactcggcgt tcgcaatggt gtaagtcggt gcggtgatgg tgccgtcagc gttgacttct
    18601 gcgtcaccac ccagcgcatc agcgatgtac ttattggtgg tatagagttg tgagccgtta
    18661 atcgcgtcgc tgctggcagc agagattgca ccgttagcga cgttagtgat tacactggca
    18721 gttttatctt ttccgtgagc ggcgctaaat gcaccatttt caccggcgtc cgcatcccag
    18781 agcagagcgt cgtcaagagt ggtatcgata gcattcaggg catcaccgac attatcgtaa
    18841 tcagcattag caatggtgta cgtcggcgca gtgatggtgc cgtctgcatt gacttcggca
    18901 ccacccccca gcgcatcaac aacataactg ctcacgccgt ggagttgtga gccgtttact
    18961 gcgtcagaac tggagtctga aatttcacca tctgcgacat cagtgatcac gcttgcgtca
    19021 ccattagtac cgtgtttggc actgaattta cctgcggtgg catcccaaag cagagcatcg
    19081 ccgagagacg tgctaaatga ggagttaatc gcagccagtg catcgccgac gttataataa
    19141 tttgtttcac cgattttgta ggttggaccg gtaaacacac cgtcagtatt cacagaagca
    19201 ttgccgccga agtaggtggc gatattgctg cttaagtcat aaagctggct accgttaatg
    19261 gcatcggaac tggttgcagt gactgtgccg tccaggatat tggtgatttt gctggcggta
    19321 ttgttgccat gagctgcagt gaagacacca ttgtccttat cccatttcag cgcatcctca
    19381 ccaagattag tcaccgtctc agtcagatta gagatgttgg tggtgttagt ggcgatattg
    19441 gtggtattcg tcgccacggc atcgttggtg gttttcagct gagagccgtt aacggcatcg
    19501 gtgctgccag tcgtcaggtc gccatcttta acgttggtga ttttgctggt ggtctcggtg
    19561 ccatgagctg ccgtgaatac accattgtcc ttatcccatt tcagcgcatc ctcaccaaga
    19621 ttagtcaccg tctcagtcag gttagagatg ttggtggtgt tagtggcaat attggaagtg
    19681 ttattggcga tattggtggt attcgtcgcc acagcatcgt tggtggtttt cagctgagag
    19741 ccgttaacag catcagtgct gtcagccgtc aggtcggcat ctttaacgtt ggtgattttg
    19801 ctggtggtat cctggccgtg agctgcactg aatgcctttt tagtttcgtt ccagagcagg
    19861 gcatcagcct gaaggtcacc aacggaatcc gtcaggttgg tgatattggt ggtattggtg
    19921 gcaatatttg ccgtattcgt ggcaatgttg ctggtattag tagcgatatt ggcggtgttg
    19981 gcttcgacat cgtcattggt agctttcagt tgggaaccgt taaccgcatc cttactggag
    20041 gctgaaatcg tgccatccgc aacatcggtg attacgctgg cagttgggct actagtacca
    20101 tgagcagcgc tgtatttgcc tttggtttgg tcccattgca gggtgttttc atcaagtaca
    20161 gcgagcgcag cccctacgtt atttttgcta ccatttttta aattgtaggt tggtgctgta
    20221 acagtaccgt catcaacatc tactgcagcc cctcctccaa gcctttttgc taccgagtcg
    20281 ctaatggcat aaagctgtga accattaatg gcatcgtaac tgtcagactt gatcgcgcca
    20341 tttttcacat taacgatctt gcgttttaat gaactattgc cgacagagac ggtattgtct
    20401 aaatccgcaa tggaatctgc gcccagcgcg acagagttaa taccgttggc actgctatta
    20461 ctaccaagcg caagactctt atcaccagtt gcattactgg ctgtgcctaa ggcaatggtg
    20521 tttaagcctc tcgactggct accattaccc agagcgatgg catccacgcc atcagctttg
    20581 ttgccctggc ctatggcgat tgcattatct gccagagcct ggctgccatt tcctaatgcg
    20641 atagtattga ctttatcggc aaggcttccg aaccccattg ccatgctatt agtccctttt
    20701 gccttcgcat tattgccaat agcagttgca ttttcagcgg cttcggtctc agcacctatc
    20761 gcaatagcgt tttcttcaga tgcgacgcta cttgctccca gcgccattga atacgctttt
    20821 gacgcattgg cggtgtcgcc aagagcaata ctcataatct cagtagcttt agtggcattt
    20881 cccagggcaa tggcgtttgc tccttccgct ttggaagagt cccccatagc aatagacaat
    20941 ttacccaatg cttttgaata acggccgagg gccattgagt attggcccaa tgaatacgaa
    21001 gaggcaccca gtgctatact tctatcgcct tctgatattg cactaacccc aaaggccatt
    21061 gatgcaccac caatcgcatg ggtttttgac ccgatggcag agctatattg gccttcagct
    21121 atagcgtcat aacccacagc agtactggaa ccactggtgt tcataaaagt atttgctttc
    21181 gcccctttgc ctatagcaac ccagccgtcg ccagctgatc cactaccgta atcaacaccc
    21241 tgaccgttgt cattcccggc attcgccaat gccccaaacg acgacaacat tccacccgca
    21301 accagcgcag aaattaacag cttactgcgc ccagatttct tgccacggct ttttgccgtt
    21361 tcgctggtaa cagtataatt ccctgtcgca gggttccaga taactttaaa tattttgttc
    21421 attaatgatg ctcgctattc cttgttataa gcgtactaac tgcatttatt ttttgcttct
    21481 tttctgccga ttttcagcag agggaagatt gtcgaactga atattttcca tgttgatggc
    21541 taacttcaga tatcccaggt tattgggcga tacacccttc agggtcaaat taatgtcttg
    21601 ccattgacct ggtaataatt tcaatgtgga tttcattgat gtgttgaaaa gtaattgttg
    21661 ccaccgggca aactctttgg catcgacagg tttttcacca ccccatgtcg cattaaaacc
    21721 aatatttgag ataggtgtac ttaataagtt gaccagtgaa atatgaataa cactgccatt
    21781 ctctgtggcg tcaatggata acactcgacc cgctaaagcc accgaaccat tgttcaggat
    21841 gaaatacttc gtttttgctg ggttaaactc ggtaaaaata aaagtgtttt gttgatcttt
    21901 aacggattta attacgtgag ttaaagtctt tgtactgctt attatgccag tttgttgttt
    21961 ttgaatagtt tccagttgac tctttagctg tgcttgttgt ttttcaagta gatcaatttt
    22021 attgttatgt tcttcttgtt gtttcaataa actactttct ggcttgcctt cttcaatgta
    22081 gtcacatccc gttaatgcgc agagaactga aacagtgaca gcactgaaaa aaatcttctt
    22141 ctagttcatg taaaaatcct tatttcctcc gcccagcgat caatgccggt agagttgtcc
    22201 attgtattat tggtgatcta tgttgcttta cattaatgac agtgatgtgt gtcgat
//
LOCUS       DAZFTN010000078        21868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFTN010000078 DAZFTN010000000
VERSION     DAZFTN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            328..534
                     /locus_tag="ACHKV2_003300"
     CDS             328..534
                     /locus_tag="ACHKV2_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO6941131.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            610..906
                     /locus_tag="ACHKV2_003301"
     CDS             610..906
                     /locus_tag="ACHKV2_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6941132.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            912..1697
                     /gene="bet"
                     /locus_tag="ACHKV2_003302"
     CDS             912..1697
                     /gene="bet"
                     /locus_tag="ACHKV2_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6941133.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1694..2371
                     /locus_tag="ACHKV2_003303"
     CDS             1694..2371
                     /locus_tag="ACHKV2_003303"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6941134.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2371..2553
                     /locus_tag="ACHKV2_003304"
     CDS             2371..2553
                     /locus_tag="ACHKV2_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO6941135.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2526..2717
                     /locus_tag="ACHKV2_003305"
     CDS             2526..2717
                     /locus_tag="ACHKV2_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO6941136.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            2794..3009
                     /locus_tag="ACHKV2_003306"
     CDS             2794..3009
                     /locus_tag="ACHKV2_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6941137.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3108..3329
                     /locus_tag="ACHKV2_003307"
     CDS             3108..3329
                     /locus_tag="ACHKV2_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6941138.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            3326..4273
                     /locus_tag="ACHKV2_003308"
     CDS             3326..4273
                     /locus_tag="ACHKV2_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6941139.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            4275..4451
                     /locus_tag="ACHKV2_003309"
     CDS             4275..4451
                     /locus_tag="ACHKV2_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941140.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            4785..5141
                     /locus_tag="ACHKV2_003310"
     CDS             4785..5141
                     /locus_tag="ACHKV2_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941141.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            5138..5500
                     /locus_tag="ACHKV2_003311"
     CDS             5138..5500
                     /locus_tag="ACHKV2_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6941142.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPNDKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEP
                     ASKVTHWMPLPEPPQEVK"
     gene            5588..5830
                     /locus_tag="ACHKV2_003312"
     CDS             5588..5830
                     /locus_tag="ACHKV2_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6941143.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            5834..5968
                     /locus_tag="ACHKV2_003313"
     CDS             5834..5968
                     /locus_tag="ACHKV2_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941144.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            5987..6241
                     /gene="xisR"
                     /locus_tag="ACHKV2_003314"
     CDS             5987..6241
                     /gene="xisR"
                     /locus_tag="ACHKV2_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6941145.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            6275..7561
                     /locus_tag="ACHKV2_003315"
     CDS             6275..7561
                     /locus_tag="ACHKV2_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6941146.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            7582..8283
                     /gene="mlrA"
                     /locus_tag="ACHKV2_003316"
     CDS             7582..8283
                     /gene="mlrA"
                     /locus_tag="ACHKV2_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO6941147.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            8343..8450
                     /gene="yohO"
                     /locus_tag="ACHKV2_003317"
     CDS             8343..8450
                     /gene="yohO"
                     /locus_tag="ACHKV2_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO6941148.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(8431..9162)
                     /gene="yehW"
                     /locus_tag="ACHKV2_003318"
     CDS             complement(8431..9162)
                     /gene="yehW"
                     /locus_tag="ACHKV2_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO6941149.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(9167..10093)
                     /gene="yehX"
                     /locus_tag="ACHKV2_003319"
     CDS             complement(9167..10093)
                     /gene="yehX"
                     /locus_tag="ACHKV2_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO6941150.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(10086..11243)
                     /gene="yehY"
                     /locus_tag="ACHKV2_003320"
     CDS             complement(10086..11243)
                     /gene="yehY"
                     /locus_tag="ACHKV2_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO6941151.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(11250..12167)
                     /gene="osmF"
                     /locus_tag="ACHKV2_003321"
     CDS             complement(11250..12167)
                     /gene="osmF"
                     /locus_tag="ACHKV2_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO6941152.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(12419..14716)
                     /gene="bglX"
                     /locus_tag="ACHKV2_003322"
     CDS             complement(12419..14716)
                     /gene="bglX"
                     /locus_tag="ACHKV2_003322"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO6941153.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14912..16627
                     /gene="dld"
                     /locus_tag="ACHKV2_003323"
     CDS             14912..16627
                     /gene="dld"
                     /locus_tag="ACHKV2_003323"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6941154.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(16665..17597)
                     /gene="pbpG"
                     /locus_tag="ACHKV2_003324"
     CDS             complement(16665..17597)
                     /gene="pbpG"
                     /locus_tag="ACHKV2_003324"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO6941155.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(17771..18358)
                     /gene="yohC"
                     /locus_tag="ACHKV2_003325"
     CDS             complement(17771..18358)
                     /gene="yohC"
                     /locus_tag="ACHKV2_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO6941156.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            18528..19106
                     /gene="yohD"
                     /locus_tag="ACHKV2_003326"
     CDS             18528..19106
                     /gene="yohD"
                     /locus_tag="ACHKV2_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6941157.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(19236..19997)
                     /gene="yohF"
                     /locus_tag="ACHKV2_003327"
     CDS             complement(19236..19997)
                     /gene="yohF"
                     /locus_tag="ACHKV2_003327"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6941158.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(20050..21486)
                     /gene="mdtQ"
                     /locus_tag="ACHKV2_003328"
     CDS             complement(20050..21486)
                     /gene="mdtQ"
                     /locus_tag="ACHKV2_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO6941159.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            21710..21793
                     /gene="yohP"
                     /locus_tag="ACHKV2_003329"
     CDS             21710..21793
                     /gene="yohP"
                     /locus_tag="ACHKV2_003329"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO6941160.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         5599 a         5636 c         5540 g         5093 t
ORIGIN      
        1 cattatcaat actaattgct attgactaca ccaatagcca ttgctattgt catgctattg
       61 catcgatgtt ggtatcacaa aatttataac ttcaccgttg cgatgaccgc ttagatccgc
      121 agcttgaatt tcagcaggct ccggggagtg cgaggggtga agcggacgcg tgaacgtcgg
      181 tgtgaccagc tgaaatcaac tcaacacttc atacctcagt cgcttcaacg aggcgactta
      241 gttatgacaa ccggcggcca tccaccgcct gaatacgcgc agaagtctct atatgttcag
      301 cagcccagct tacgggcagg agtttttatg gttcatcaac attacggaac gcagaccgtt
      361 aatcgaggtg cggtcatgcc aggaatgctg gtcaaacaca aagatggtac ctggactgca
      421 tcagctaatt tacgcggacg gctttatctg catcgcggca tcgagcgcac ttatacccgt
      481 gatttgctcg tggaagtttt tctcgacgga cgcggtaacg gcctgaatcg ctaatcccct
      541 ttcctgtttt cctaatcagc ctggcatttc gcgggcgata ttttcacagc cattttcagg
      601 agttcagcca tgaacgctta ttacattcag gattgtcttg aggctcagag ctgggcgcgt
      661 tactaccagc agatcgcccg tgaagagaaa gaggcagaac tggcagacga catggaaaaa
      721 ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc
      781 agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga gcgcatggca
      841 gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat tgattcagag
      901 gtataaaacg gatgagtaca gcactcgcaa cgctggcagg gaagctggct gaacgtgtcg
      961 gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg gcatttaaag
     1021 gtgatgccag cgatgcgcag ttcatcgcat tgttgatcgt cgccaaccag tacggcctta
     1081 atccgtggac gaaagaaatt tacgccttcc ctgataagca gaacggcatc gttccggtgg
     1141 tgggcgttga tggctggtcc cgcatcatca acgaaaacca gcagtttgat ggcatggact
     1201 ttgagcagga caatgaatcc tgcacatgcc ggatttaccg caaggaccgt aatcatccga
     1261 tctgcgttac cgagtggatg gatgaatgcc gccgcgaacc attcaaaacc cgcgaaggca
     1321 gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat aaagccatga
     1381 ttcagtgtgc ccgtctcgcc ttcggatttg ctggtatcta tgacaaggat gaagccgagc
     1441 gcattgtcga aaataccgca tacactgcag aacgtcagcc ggaacgcgac atcactccgg
     1501 ttaacgatga aaccatgcag gaaattaaca ctctgctgat cgccctggat aaaacatggg
     1561 atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt gcatcgtcag
     1621 aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacaaaaa gccactgaac
     1681 agaaggtggc agcatgacac cggacattat cctgcagcgt actgggatcg acgtgagagc
     1741 tgtcgaacag ggagatgatg cgtggcacaa attacggctc ggcgtcatca cagcttcaga
     1801 aattcacaac gtaatagcaa aaccccgatc aggaaagaag tggcctgaca tgaaaatgtc
     1861 ctacttccac accctgctgg ctgaggtttg caccggtgtg gctccggaag ttaacgctaa
     1921 ggctctggcc tggggaaaac agtacgagaa cgacgccaga accctctttg agttcacttc
     1981 cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc gcaccgcctg
     2041 ctctcccgat ggtttatgca gtgacggcaa cggccttgag ctgaaatgcc cgtttacctc
     2101 ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaaatcgg cttacatggc
     2161 ccaggtgcag tacagcatgt gggtgacacg aaaagatgcc tggtactttg ccaactatga
     2221 cccgcgcatg aagcgtgaag gcctgcatta tgtcgtgatt gagcggaatg aaaagtacat
     2281 ggcgagtttt gacgagatgg tgccggagtt catcgaaaaa atggacgagg cactggctga
     2341 aattggtttt gtatatgggg agcaatggta atgaagcatc ctcacgataa tatccgggta
     2401 ggcacgatca ctttcgtcta ctccgttaca aagcgaggct gggtatttcc cggcctttct
     2461 gttatccgaa atccactgaa agcacagcgg ctggctgaag agataaataa taaacgaggg
     2521 gctgtatgca caaagcatct cctgttgaat taagaacgag tatcgggatg gcacatagcc
     2581 tcgctcaaat tggagtcagg tttgtgccaa taccagtaga aacagacgaa gaatttcata
     2641 cgttagccac atccctttca caaaagctgg aaatgatggc ggcgaaagca gaagcaaacg
     2701 agagagaccc ggcatgacaa caacagaatg catttttctg gcagcaggct tcatattctg
     2761 tgtgcttatg cttgccgaca tgggacttgt tcaatgacac ctcagcagga aaacgccctt
     2821 cgcagcattg cccgtcaggc taattctgaa atcaaaaaag ccagacagca gtttccggat
     2881 aaaaacgtcg atgacatttg ccgtagcgta ctgaagaagc accgcgaaac ggtaacgcta
     2941 atgggattca caccgactca tttaagtctg gcgatcggca tgttaaacgg cgtctttaag
     3001 gagcgatgaa catgaaaagc aaaatcatca gggagctaca ggctcctttt ttattgttcg
     3061 cattcaccct caagcgtatt aaccaacaat tcagggatta atggaagatg gcagacatca
     3121 ttgattcagc atcagaaatt gaagaattac agcgcaatac agcaataaaa atgcgtcgcc
     3181 tgaactacca gactgtatcc gcaactcatt gttgtgagtg tggcgatccg atagatgaac
     3241 gaagacgcct ggcagttcag ggttgtcgga cttgtgcaag ttgccaggag gatctggagc
     3301 ttatcagtaa acagagaggt tcgaagtgag cgaaattaat tatcaggcac tgcgtgaaaa
     3361 ggcagagaaa gcaactaaag gaagctacat cgtagggcat acatctgtta accagcacgg
     3421 caatttaaca ggagtttttg tttgccaaaa atggaaagga gaacccggtg gtgtgattgc
     3481 ggaatgtcat gttaactgcc tggttgaaac agatgttcag gcttatgcaa acgctgaatt
     3541 tattgctgcc tttaatccaa atgttgcgct ggcgcttctg gatgaacggg aaagaaacca
     3601 gcaatacatc aaacgccgcg accaggagaa cgaggagatt gcgcttacgg ttgggaagct
     3661 gcgtgttgag cttgaagcag caaaatcaaa actcaacgag cagcgtgaat attacgaggg
     3721 agtaatcgcg gatggaagta agcgcatagc agaactggaa aaacaatgcg ccgaatggga
     3781 gcgaaaagca ttaagcaact ttgaagagtg tgctgcgatg gctgaacgta tcgaagagat
     3841 gcagacaaaa tctgcaccag attcgtttgg catcatcggt gaaaatattc gaacacagga
     3901 caatcgaata acgtcagatc ccatgttttg tgtgtatcaa aagcgcgaaa tcgctgttga
     3961 tgctgattat gaccatgacc ggattgtctg ggttgacgaa gatggcaatg aagccaataa
     4021 acgccatagt cgtcgtctcg agctacttca tgaaaacttt cgagagccac cagaaaaatg
     4081 gcggcgcgtt gctgtgaaag atattgatga attcgttacc tgctgtttca ccgaacaggg
     4141 ttgtaaagac tacctggcag tcaatggtca caatcttcgc ttgccattta tatatgtaaa
     4201 aagcggtttc aggaacgctg aatatatcgg cataagaaac tggcttgctg gcattcgcat
     4261 caaaggagag tgatatggcg ttaacacacc gcgaactctg tcagattgcg tacaagttcc
     4321 ttaagcgcaa cggtttcaag gtttgttttc atgaccgctt tatagctgta accagtaccg
     4381 gagaacagcc agatgctatg ggattcagaa attcagcatc atgcctgatt ggcgaaatgt
     4441 tctcgtgctg acttgttggc agatagaaaa aagcgttttc gtaaaaatcc gtctcttgga
     4501 atgggcgact ggcgattctt tattagtgag ccgggaatta tttcaattga ggatttacca
     4561 cctggctggg gattacttca cgttgttaac ggaagagtac ggaaagtaca tgggtggccc
     4621 aagggtaatt gctgttgggg taatcctgac gataagccat ttactggaaa taagcaggtt
     4681 gaatgcgatt acatgttgtc tgcattaagg cgcatggagt tgagagggca ccttaatgaa
     4741 atatatgacg gtgtaattgt taataagaaa gaaggaaacg cggcatgacc actattaccg
     4801 acaaagaact gattaaagaa atcaaagagc gcataggcag cttggacgtt cgagacaata
     4861 ttgagcgccg tgcttatgaa attgcactgg catcgctgga agcagaaccg atagcgtggg
     4921 aatgcggtga aaacataatc ctgtttaacc ctgacacagt tgaagcatac gcaaaacgtg
     4981 cggaaatatc acctaaacca ctattctccg cgccgccagc gctggtagtg cctgataagt
     5041 tgccgcgtga atacagaaac ggttggcctc ttgcgtatag tgattatgct gaaggctgga
     5101 acgactgccg cgaagccatg cttcagggag ataaatcatg attaatcgta tcaagctgga
     5161 gcacatcctc gaatatgcca ggcagcagag gcatattggt cagcattgta aaattccacc
     5221 aggagatatg gttgaaatca tggagattgc catgcgcaag gctggcaact ctccggtaac
     5281 tccggatggt tggataagct gtagtgagcg aatgccgaac gataaacagt atgtttggtg
     5341 ttgggggaag tcttacggct ggactgagtg cgataccttc gaagggtatt acgattgttc
     5401 gagaaacaaa tggtgggcag ttactgacaa tggggaagaa ccggcatcga aagtaaccca
     5461 ctggatgccg ctaccggagc cgccgcagga ggtgaaatga tgaattggcc tgaagcattc
     5521 accgcagttg gagttgcaat agcggtggca tttattctgt attcgctttt ccgctgggga
     5581 taaaggaatg ttcgctctga ttcaacgtgg tcagatatac acggacagag ccggataccc
     5641 tgtggtgatt actcgcagta ctcagcactc agtgttcttt cgacgcatgg acgggcgctc
     5701 cggacgggta cgcattggtg agttcaacaa cctgttcgaa catattgacc aacaggagta
     5761 ccgcaaaatt ctggcgggca ctgagcagga aatgcgcctg aaaaaattac gcgcaatgca
     5821 acggaggtga tacatgcata cggcttttga gttctgggtt cgcaagacat tcggcaatcg
     5881 ctacgacctg acccgtgatg tcgacggctt ctactgccgt gaagttgtga aacgaatgtt
     5941 tgacgtgtgg tgccactgcc gtggatgaaa gttttatgag gttggcatgc agacaatcat
     6001 ctatcagata acccccagca aatggtgtac ggagagagtc cttattgcat caacagggct
     6061 aaagcccggc accatcgagc gggccagaag aaagtcatgg atgcagggaa aagaataccg
     6121 ccattacgct gtagaaggtg atcctgggca ttacagtgaa tgcctgtaca acatcgaaga
     6181 aattatgcga tggatcgaaa accagaaaca accaggtgcc aaaaatgcaa gttccggtta
     6241 acctgttaat gctcctggac gtctgggagg tttaatgagt aacgcatcat acccgacagg
     6301 cgttgaaaac catggcggat cactccgtat atggtttcac tataatggca aacgtgtcag
     6361 agaaaacctc ggtgttcctg acacagccaa aaaccggaag atcgctggtg aacttcgcac
     6421 ttccgtttgt tttgcaatca gaatggggag tttcgactac gccgcgcagt tccctaattc
     6481 ccctaacctg aaacactttg gtctgggaaa aagagagata accgttaagg cactttcgga
     6541 aaaatggttg gaccttaaga aaattgagat ttgtgcgaat gcacttaacc gttaccagtc
     6601 agtaattaaa aacatgttac caatgttagg tgaaaaaaaa ctggtttcat ccataacaaa
     6661 agaggattta cttttcgtaa ggagagattt gttgaccggt taccaaaagc tttctaatgg
     6721 aaagacttct tccataaaag ggcgctcagt ggtcacggta aactactata tgacaaccat
     6781 agctggaatg tttcaatttg caacagataa tggttatacc tcaggaaacc catttaacgg
     6841 tctggctccc ttaaaaaagt ccaaggtaaa accagatcct ctcacccgtg acgaatttat
     6901 tcgttttatt gaggcttgcc gtcatcaaca aacaaaaaac ctgtggattc tcgctgtata
     6961 cacgggtatt cgtcacgggg agttggtatc gctggcatgg gaagatatag accttaaagc
     7021 aaggactata accatccgta gaaattatac aaaacttggc gaattcactc caccaaaaac
     7081 cgatgcaggc accggaagga caattcatct ggttcaacca gctattgatg ctcttaaaag
     7141 ccaggcggaa atgaccatgc ttggaaagca acattctgta gaggtgaagc agagggaata
     7201 tgggagaact gctgtgcata aatgcacttt tgtttttagt cctcaggtaa caaaacagca
     7261 gcagttgtcc ggacctcact acaaggttga ctccatcagg gagtcatgga caagtatctt
     7321 aaaacgcgca ggtctgagac acagaaaatc gtaccaatcc aggcatactt atgcatgctg
     7381 gtcacttgcc gcaggagcta atcctagttt tatcgcaagc cagatgggcc acacaaacgc
     7441 acaaatggta ttcaatgttt acggagcatg gatgaaagac aacaatcacg aacagataga
     7501 actccttaac aaaagactat ctgaaagtgt cccatgtatg ccccataaga aagcagggta
     7561 aaataaaaac ttgcaaaatc aattagttta cccttaatcc ctgtcacgtt acgcgcgtgg
     7621 cagaggcgtt acggattgct gaaaccgcaa cggacagacg gcggtcatcg gctgttcaac
     7681 gatgccgata ttgaccggat ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc
     7741 agcaaagtta aaatgctgct cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat
     7801 cagcaagaaa cattactgac ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg
     7861 atcaaagagc gcggtcagga ttaccccgcc cagacgctca ccacacatct gtttattcct
     7921 ctgcgccgac ggcttcagta ccaacaaccg actctccagg cgctgctggc gatcctcgac
     7981 ggcgtactga tcaactacat cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa
     8041 gatgcgctgg tggttggctg gaatattcag gataccaccc gtctgtggct ggagggctgg
     8101 attgccagtc aacaaggatg gcgcattgat gtcctcgccc actcgctcaa tcaactacgc
     8161 cctgaactat tcgaaggccg tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc
     8221 caacagcagc aactcaccag ttggcaagaa caaggccatg atattttccc actcggcatt
     8281 taatgattcg ttaacaaatg cgctttactg tacaatcctt tcgttaacat aaggagtgca
     8341 ttatgcgcat agctaaaatt ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa
     8401 ttggtggcgt catgctcgca ggttatacct ttattttgcg tgctggctaa gcgcctgcac
     8461 cagcctttca aacaggcggt ctgcgatgat cgccgccagt gccaccagta acgccccctg
     8521 gatcacatac gcggtattaa atccgctaag cccgatgatg atgggcgtac ccagcgtgct
     8581 ggcccctacc gttgaggcga tcgtcgccgt accaatgttg ataatcaccg aggttcgcac
     8641 gcccgccaga atcaccggag ccgccagcgg tagctcgacc ttacgcagtc gctgacctcg
     8701 actcattccc atacctttcg caacttctgt cacgctggca tcaatcgctc ccagcccggc
     8761 aagtgtcgcc tgcaggacgg gcagcacacc ataaaggatc aaggcgataa tcgctggttg
     8821 cagaccaaag cctatcaccg gaacagcaat cgccagcact gcgacaggcg gaaaagtctg
     8881 cccaacggcg gcaatagttt ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac
     8941 tgcaattccg gcaccagtgc caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca
     9001 gaaatgagcc agtgccaaag ctgcaaaact ttcttgctga taaacgggtc gtggcagttg
     9061 tgggaacaag gcagcaaaca gcggctggct gtaaggcagc cagaaaatca gtgccacaaa
     9121 caaagcaatg agccagaaca gcggatcgcg caacatcttc atacgcttac gcctccacca
     9181 gcagatcctg aaaatgcagc gtgccgctag gctggccctg cgtgttcacc accggcagca
     9241 cctcgcatcc ccgcgcgaca aacagggaga gcgcatcgcg cagcgtcatc tcttctgcca
     9301 gtgcctcacc ttctgcccgt tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa
     9361 gcaggcgcac acccagttca ctacgtccaa aaaactggcg gacaaaatca ttcgccggac
     9421 gagtcagcat cgtcagcgga ttcccctgct gcaccacttc accgtgatcc atcaatacca
     9481 gatgttctgc cagccgtagc gcctcatcaa tatcatgcgt gaccagcaca atggtacgcc
     9541 ccagcaaacg gtgaatgcgc gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca
     9601 gtgcgccaaa aggttcatcc atcagtaaga cttgcggatc ggcagccagt gcgcgcgcta
     9661 ctcccacacg ttgctgctga ccaccggaaa gctgatgtgg ataacgctca cgtaaatttg
     9721 actccagccc cagtagcgcc attaattcgt cgatacgatc gtcaatcctc gcccgcgacc
     9781 atttttgtaa ttgcggcacg gtggcaatgt tttgtgccac gctccagtgg ggaaacaggc
     9841 caatagattg aatggcatag cccatacggc ggcgcaactc cagtactggc agcgagcgaa
     9901 tttcttctcc agcaaagcgg agctctccgc tgtcatgttc caccaggcgg ttaatcattt
     9961 tcagggtggt ggatttgccg gagccagatg tgccaatcag caccgaaaaa ctcccttcct
    10021 gaaaattgag attgagatcg ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc
    10081 taaattcaat cattacgttt caccttcaac agtgcgatta gtaaatcgaa cagcgcatcg
    10141 atcagcaccg ccagaacaat taccgggatc acccccagca acactaaatc aatggcgctg
    10201 cttagcagcc cctggaaaac cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc
    10261 accgccatgc ctacagtttg caccatcacc acccgcaggc tgcgcagaaa taccggtaac
    10321 gccagcggta actgaacatg caggaatcgc tgcgccccgc tcatgcccat cgctctggcg
    10381 ctctccagca catcgcgcgg gatctggttc aagccgacta ccacgccgcg caccagcggt
    10441 agcaaggcat agagcaccag cgcaatcagt gcgggtgtca ttccggttcc tgctatgcca
    10501 agcttcccca gccacggaaa ggccgtaacc agtccggcaa gcggcgcaat cagcaggcca
    10561 aagagcgcca cggaaggcac ggtctgaatg acattgagca gagaaaaaat cgccccctgc
    10621 cgcgcggtgg aaaagtagca ccagatgccc aacggcacac caatcactaa cgcaggcagc
    10681 accgcaccaa acagcaacgt cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg
    10741 ttggcgtatt ctttcattag tgagagatcg ttaagcgtgc cggagtacag caaccacagc
    10801 ggaataatgg caatctgcat atgcaacaac cagcgccaca gcggatgcgt ggagattcgg
    10861 cggatggcat cgctacaggc cagcaatgcc agcgccgaag ccaaccagaa accactgccg
    10921 aggctggtac gcgccagcgc actgccattt tgcgccagtt gggtcgccgc ctttccagct
    10981 ccccacacca gcaatacgaa gacgaattgc gccagaatga gtgcaaaaat gctgcctttt
    11041 ttagcgggaa taaaacaggc tgtcagccag gcgcaaccaa cgcccaccag catccagatc
    11101 gtttgcggcc acagttgcca gagatgacgc ccttcacccg agactaaacg attaggcgcg
    11161 tagctgataa acggcagcgc tgctgcgatt gccgtcagca acagcagcag cgccagaaca
    11221 ggattaatac gtagataagt cacgggaaat tacttcgtcc accctttttg tttcaagtag
    11281 tcggcagcca cttttttggc atccagtcct tccactgcaa tgctggcatt cagttgctgc
    11341 aatgtttttg catcgaggct ggcgaagact ggctgtagcc actgtgccat ttgcggatac
    11401 tctttcagca ccgactcacg caccactggt gcaggcgcgt agataggttg cacgccttgc
    11461 ggatcgctta aggtttgcag cccctgcgcc gcgaccggac cgtcagtgcc gtaagccatt
    11521 gcggcattaa cgccagaagt ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg
    11581 ccagccagtg acagtaactg atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg
    11641 ggtaacgcat cggcgcgttc gataaactca gccgaggctg ccagtttgaa ggtgccgccc
    11701 tctttcagat aacgcctcag gtcagcaagc gaagtgagtt tgtttttctc tgccacatcc
    11761 tgacgcacgg cgatggtcca ggtgttattt gcaggtgcgg gcgtcagcca gattaacttg
    11821 ttttgctctg catcgagttt tttgactttc tcgtaacctt gctgcgcgtt tttccacgct
    11881 gcatcgtttt catctttaaa gaaaaacgcg ccattgccgg tatattccgg atagatatcc
    11941 agttcaccgg aagtaatcgc cccgcgcacc acaggagtcg ttccaagttg cactttattg
    12001 accgttggta ctccgtggct ttcgagtact tgcaaaatga tattgccgag cagcgcgcct
    12061 tcggtatcga tttttgaacc gactttaacg ggggaagccg cgtgcagcgg caggctcacg
    12121 gctgccaaca aaaccagtga acctgcccag acctttgaga gtggcatgat gctttcctca
    12181 ttcttttact gttgttttca gcgaattaag agaaaagcat agttgataat ggcgggatta
    12241 gcctgagcgg acggattcag ttgcagaatc agataaatac ttaagaggca tattcggtcc
    12301 ggcgtttcct gccggatgcg gcgcgagcgc cttaaccggc ctacaaaggg cgaaaattca
    12361 gcaagttaag atatgaacat caggcaaagg gcgcaaacgt cgcgcccttg ctaaagcatt
    12421 acagcaactc aaactcgcct ttcttaacgc gtgcggaatc agtgccgata aagacattga
    12481 acttgccagg ctcggcgtca tatttcatct gttgattcca gaacttcagc gcctcgatat
    12541 caatcgggaa gctgacggtc tgggtttcgc ccggcttcag ggtgattttc tcaaagcctt
    12601 tcagctgctt cactggacgg ctcatggaag ccgtcacatc ctgcaagtac atctgcacta
    12661 ccgtggcccc ttcgcgttta ccggtgttcg tcacctgcac gctggccgtc actttgccgt
    12721 cacgcttcat ggtcggcgca gaaagtttca catcagagac ggtgaaagtg gtatagctca
    12781 gaccatagcc gaacggataa agcgccccgt tagcttcatc aaaataacgc gaagtgtatt
    12841 tgttcggctt gtcggcatta tacggacgac cggtgttcag atggctgtag tacaccggga
    12901 tctgcccgac agaacgcggg aaggacatcg gcagcttacc ggacgggttg taatcgccaa
    12961 acaacacatc ggcaattgca ttaccgcctt cagtcccggc aaaccaggtt tccagaatcg
    13021 catcagcctg ctgatcttct ttcaccagcg ccagcggacg cccgttcatc agcaccagca
    13081 ccagcggttt gccggtggct ttcagggcgg caatcaaatc acgctggctt tgcagaatag
    13141 taatatcggt acggctggag gcctcgtgcg ccatcccctg tgcttcaccg actacagcca
    13201 ccaccacatc agattgtttc gccgtctgca ccgcttcatc aatcatctct tgcggcgaac
    13261 gcggatcgac tttgaccgct tcttcatact gattcaggaa atcgataatg cctttgtcac
    13321 tggtaacgtt cgcccctttg gcatacagca ctttaccgtt ttcaccgacg gcatttttaa
    13381 tcccggtcag cacggtcacg gattgatcgg caacacctgc cgccgaccag ctgcccatca
    13441 cgtcacgttt actgtccgcc agtggcccaa ccaccgcaat ggtggctgat tttttcagcg
    13501 gtaacgtttc gagacggttt ttcagcaaca ccaggctttc acgcgccact tcacgcgctt
    13561 ctttgcggtg caggcggctt tcggcattgg tatccaccgg gtcagactct ttcggaccga
    13621 gatggctgta tgggtcgtta aacaacccca tatcatattt aacgttcagt acatggcggg
    13681 cagcatcgtc cagctctgcc atcgtcactt taccggattt gatcaaccca ggcagatact
    13741 tcgagtagta ctcgtcgctc atgctcatgt tgattccgga tttcagcgcc acgcgcactg
    13801 catcttccgg atctgccgcc gtgccatgtt taatcagctc tttgattgca ccgtgatcgg
    13861 aaacggtgat gcctttaaag ccccactggt cgcgcagaac atctttcagc agccaggagt
    13921 cggaggtggc tggcgtgccg ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg
    13981 cgtccagccc cgctttgtac ggcggcatat aatcattaaa caggcgctgc ggactcatat
    14041 cgacggtgtt gtactcttta ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc
    14101 tggtcatcac cgagtaacga tctgccgggc ttttaccctg catcgcttcc accatggttt
    14161 tacccattat tgaggtgaga tacgtatctt cgccaaaacc ttcggaagca cgtccccagc
    14221 gcggatcgcg cgagacatcg accatcggtg cccaggtcat attcaggcca tcatctgccg
    14281 cttcataagc agagacacgt ccgaccgttt ttactgcatc gaggttaaaa gacgaggcca
    14341 gaccgaggct aatcgggaac accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa
    14401 gaggaatttt caggcggctt aattccatca cctgatcctg catggcgcgg atatcctgac
    14461 gggttacggt gttgaaaata gccccaacct gaccgtcttt gatcatctcg cggatcgcct
    14521 ctttcgggtt atccgggccg acgctgatta agcgcagctg accaattttc tcatcaactg
    14581 tcattttctt aagcagttcg gtgacgaacg catcccgcgc ttcaggcgtt aatggatggt
    14641 taccgaataa atcatccgcc agtgctggct gcagggccag actcaccgcg attcctactg
    14701 aacatagcca tttcatatgg atttttcttc ctttgttgcc cgacgtggca gcgaaaatgg
    14761 tgcaaaaacc gtagtttgcc ataagcatga tggagagaga aaaagaatgc tcagtttatt
    14821 gtctgaattt tcaaaatatt cactcgctga attgtcatac aaggcgctat tctagtttgt
    14881 gatgtttttt cgccaccaca aggagtggaa aatgtcttcc atgacaacaa ctgataataa
    14941 agcctttttg aatgaacttg cccgtctggt cggtcattca cacctgctca ccgatcccgc
    15001 aaaaacggcc cgctatcgca agggcttccg ttctggtcat ggcgacgcgc tggctgtcgt
    15061 tttccctggc tcactactgg aattgtggcg ggtgctgaaa gcctgcgtca ccgccgacaa
    15121 aattattctg atgcaggctg ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa
    15181 cgactatgat cgcgatatcg tgatcatcag caccctacgt ctcgacaagc tgcacgttct
    15241 cggcaagggc gaacaggtgc tggcctatcc gggcaccacg ctttattccc tggaaaaagc
    15301 cctcaaaccg ctgggacgcg aaccgcactc ggtgattggt tcatcgtgca ttggcgcatc
    15361 ggttatcggc ggtatctgta ataactctgg tggttcgctg gtgcagcgtg gcccggcgta
    15421 taccgaaatg tcgttattcg cgcgtataaa tgaagatggc aaactgacgc tggtgaacca
    15481 tctggggatt gatctgggcg aaacgccgga gcagatcctt agcaagctgg atgacgatcg
    15541 catcaaagat gacgatgtgc gtcacgatgg tcgtcacgcc cacgattatg actatgtcca
    15601 ccgcgttcgt gatattgaag ccgacacgcc cgcacgttat aacgccgatc cggatcggtt
    15661 atttgaatct tctggttgcg ccgggaagct ggccgtcttt gcagtacgtc ttgatacctt
    15721 cgaagcggaa aaaaatcagc aggtgtttta tatcggcacc aaccagccgg aagtgctgac
    15781 cgaaatccgc cgtcatattc tggctaactt cgaaaatctg ccggttgccg gggaatatat
    15841 gcaccgggat atctacgata ttgcggaaaa atacggcaaa gacaccttcc tgatgattga
    15901 taagttaggc accgacaaga tgccgttctt ctttaatctc aagggacgca ccgatgcgat
    15961 gctggagaaa gtgaaattct tccgtccgca ttttactgac cgtgcgatgc aaaaattcgg
    16021 tcacctgttc cccagccatt taccgccgcg catgaaaaac tggcgcgata aatacgagca
    16081 tcatctgctg ttaaaaatgg cgggcgatgg cgtgggcgaa gccaaatcgt ggctggtgga
    16141 ttatttcaaa caggccgaag gcgatttctt tgtctgtacg ccggaggaag gcagcaaagc
    16201 gtttttacac cgtttcgccg ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga
    16261 tgaagtcgaa gacattctgg cgctggatat cgctctgcgg cgtaacgaca ccgagtggta
    16321 tgagcattta ccgccggaga tcgacagcca gctggtgcac aagctctatt acggccattt
    16381 tatgtgctat gtcttccatc aggattacat cgttaaaaaa ggcgtggatg tgcatgcgtt
    16441 gaaagaacag atgctggagc tgctacagca gcgcggcgcg caataccctg ccgaacataa
    16501 cgtcggtcat ttgtataaag caccggagac attgcagaag ttttatcgcg agaacgatcc
    16561 gaccaacagt atgaatccgg ggatcggtaa aaccagtaag cggaaaaact ggcaggaagt
    16621 ggagtaaaaa ttacggatgg cagagtatcg ccatccgaat tcacttaatc gttctgtgcc
    16681 gtctgccccg ccgccgccat ttgggcggct ttttgttttt tatagcttaa cgctgctgcc
    16741 ggaacaggca tcactttacc ggtttcaatc caggtacgca ggcggctggc atcggcaaaa
    16801 tgggtgtatt tgccaaacgc atccatcact accagcgcca ccggtttatt attgataacc
    16861 gtacgcatca ccagacaatg gcccgccgca ttggtaaagc cggttttggt tagctggata
    16921 ttccagttat cgcgatacac cagatgatta gtattgcgga acggcagcgt atacgtcggg
    16981 ttagagaagg ttgccatatc ttcccgggta gtacttaact gcccgatcaa cggatattgt
    17041 ttgctggcaa tgagcagttt ggttaagtca cgggcggttg aaacgttatg caccgacaat
    17101 ccggtaggtt caacaaagcg cgtgttgttc attccgagcg atttcgcttt cgcattcatt
    17161 gccttaataa aggctttgta accaccggga taatggtgcg caaggcttgc cgccgcgcgg
    17221 ttttctgaag acatcagcgc cagcaacagc atatctttac ggctgatttc gctattcagt
    17281 cgtacgcgcg aatagacccc tttcatctcc ggcgtctggc tgatatccac ttttagtttt
    17341 tcatccagcg gcagtcgtgc atccagcacg accatcgcag tcattaattt gctgatagac
    17401 gcaatcggac gcaccagatc cgggtggttc gaatagatca ctttgttggt attcagatca
    17461 acaatcatcg cgctaccgga ggcaatttcc ggttgtgaag cggtggtagc ggctgccgtt
    17521 ttcgcaacgg cctgcggtgc caaaggcaca gccagcatca gggccaggct aaataaagaa
    17581 actcgaaatt tcggcatgat gagcattcag atagtggttc acgcgcacgg gttgcgcacc
    17641 gccggagtaa ggatttactg aggctagcga cgccatcata acgagcaaaa agtgcgatcg
    17701 tcaaaggaga atcgtgagga aatgctgcat tgctgacatt tacgccagca atgcaacgtc
    17761 aaagaacttt ctagaacaaa cgataaccgt agccccatag tataacggta agggcgagca
    17821 gcacttccag taccagcacg ccaatcgcca gcgtcgaact ggagaagcta aggccttcct
    17881 ctttgttgat attcaggaag ctcggaatac caaggtaaag taggtaaccg gtgtaaaaca
    17941 gtgccaccgt gccgaccagc gcacacaacc agaccagcgg ataaagcgcc accagaccac
    18001 ttaaaaacag cggagttgca acgtagcccg cgaagaccat gcagtgcgca agtgacggac
    18061 gctgcggata attacgcgcc atccaccaga tgacccgccc catcaccgcg accccagcga
    18121 gcataacgcc ataaaacagg acagccagcg ccagtccggt aaaccaggat aacttcagga
    18181 tagtgccatc gccaaaattc cagccaatct gtgtagtgcc aatgaaggcg caaatcaccg
    18241 gaatcgccgc catcagcaaa acgtggtggg tgtaatggtg agaaatcgtt tcgttttcgc
    18301 gattaatcac ctgcatttca cgatcgggat gggaaaacag tccccagaca tggctcatac
    18361 cgcccccttg ttgtgagttc atgaacctga cagttcaagt ataagtcagc ttgtgattat
    18421 tttttgttcg cccatgtaat ttgcaccgtc tgatgtacgt cctttttcag cgtgaatgat
    18481 tcacagggtg tatgcttaca ggcaaccaaa gggagacaga ctggcctatg gatctcaata
    18541 cactcatctc acaatatggt tatgccgcgc tggttatcgg tagcctggcg gaaggtgaaa
    18601 ccgtgacttt gctgggaggc cttgcggcac atcaggggct attaaagttc ccgctggtgg
    18661 tactttctgt agcgcttggc ggcatgattg gcgaccaggt gctctatctg tgcgggcggc
    18721 ggtttggcgg taagctgtta cgccgtttct ctaaacatca ggataaaatt gagcgggcgc
    18781 agaaactgat ccaacgccat ccgtatctgt ttgtcattgg tacgcgcttt atgtatggct
    18841 ttcgggtgat tggcccgacg ctgattggtg ccagccagct gccgccgaaa atctttctgc
    18901 cgcttaatat tctcggcgca tttgcctggg cgttgatttt taccactatc ggttacgctg
    18961 gtggtcaggt gattgcgccg tggttgcaca atctcgacca gcatttgaag cactgggtct
    19021 ggttgattct ggtcgtggtt ctggtggtag gcgtgcgctg gtggctgaaa cgacgcggga
    19081 agaaaaagcc ggataatcag gcgtaaaacc attgccctgg ataaggcgtg cgcgccgcat
    19141 ccgacatccg gttcaccatg cctgatgcgg cgcttgtgcg tcttatcatg cctaccaaaa
    19201 ttttacccat tgaaatatcc acacacctca ctgcgctatt ctgggttgaa ctgcggattc
    19261 gccaacataa agccgccatc cactatcaac gactgcccgg tggtgtaatt tgcgccctcc
    19321 gaacaaagcc acgccaccag gctggcaatc tcatgcgttg cgccaaaacg ccgcagagga
    19381 atcgaaggct ccgcgtcggg cttcacgtcg ctatcatcca tgccgttcat tggcgtagcg
    19441 atcgccccag gtgcgactgc gttcaccaaa atcttatgcc tgaccaactc cagcgccatt
    19501 gctttggtta acccgccgag cgcatgttta gcggctgtgt aggcgctggc atccggcagc
    19561 ggcgtatgtt catgtaccga cgtaatgttg atgatgcgac caccctgtcc ttgtttcacc
    19621 atctggcggg cagcaatttg cgagcataaa aatgcaccat cgacatcaac ggtaaaaatc
    19681 ttgcgccact catcgaaagc catatcaaga aacggcactt tggtcattgc gcccgcatta
    19741 ttcaccagca catcaatacg ccccagccgt tgaataagtt tctccagcgc ctgcgcacct
    19801 tctggcagat tgccgagatc cagttgcacg atctccgcac gtacgccgtg gctaactacc
    19861 tcacgcgcgg tatcttttgc cccttcttca tctgagtgcc aggtaatgcc aatatcaaac
    19921 ccttgctgcg ccagtaataa cgcgcactct ttgccgatcc ccgaatcgga ggcggtaata
    19981 atcgctacct gtgccatcga gttctccact taacgctgaa taaacgttaa gtatagaagt
    20041 cacatatcat cagcgtttgt aacccccgcc caacgcacca gtgagttgaa tggaggcatc
    20101 cagccactgc ccttgcaata acaggccatt ggcccgctca cgcagcgcgg ggattctggc
    20161 ttcgctgacg cgggaaccag caatgatgcc cgcgttaaag cgggcctgcg caagacctac
    20221 cacacgcaag gcatcgcgct caatttgcgc ctgatgctgg tttttctccg ccagtgtctg
    20281 aacctgactg gctgcccgcg ccacgtcatt caccgcttca accaccgctt tgttgtagct
    20341 ggcgatagac aagttgcttt cggcttttgc gatatcgaga ttggcgttaa gacgaccact
    20401 atcgaaaatg ggtagcgtca ggcctgccgt aacgcccatt tgctgcgcgg aatgacggaa
    20461 cagatcgctt aagtgcaacg catcctgttg caggaaggcc atcaggttga tgtcaggata
    20521 aaatgccgct tttgccgcat caatggtgct tagcgatgac tcaacgtacc agtgcgccgc
    20581 ctgcaaatct gcccgccggg ccagtaagga gtaccccagt tcatcaggaa gctggcttgc
    20641 cactttcggc aacgccaccg gtttaagcgt caatgactta gtctggtgat ttgtgagtgc
    20701 aattaaccgt gcctcaataa ttttcatttt ccccgcgaca tcgttgagct gctgccgggt
    20761 tttgctggca ttaatatcgg tttccacacc ttcaactgaa gaagtaatcc cgttctgata
    20821 tagctggcga tcggtcgcga taatggtgtt ctgctctttt tcgatttgct gcaagaccgt
    20881 gtttaacgcc gcctgggttt gccactccca gtacaggcgg gctacgctgc cagccagcaa
    20941 ttggcgggtt tgctcgcgtt ccgccgcccg tgctttaacc gtacccaggc gggcagtaac
    21001 ctccgcccga ttctttcccc agatatcgag atgccagccc gccgttaagc caaaagttcc
    21061 gttggtgtac cacggaccgg tcgtacctgc ggccggatcg ttcagagcaa acggccccat
    21121 tagcccttct gccgacattt tttgccgctc catatccgca gaaaagtcga tctgcggacc
    21181 atcctgagta gcaactgcct tcgcctgggc ttcagctaac tgaatgcgct gttcagccac
    21241 ctgcatatcc ggtgcgttct gtagtgcatt gttaattaag gaagtgagtt gattatcgtg
    21301 atactccagc caccattggc tgtctggcca accatttttc agcgccgtgg gtaatgcggt
    21361 gtcaacttgt gcagcgggcg tttgctggct taacgcctgg cgggtttcat gcataggcgc
    21421 acacccggcc agcatcagta acagcggaaa acaggcgatg gctggataaa aggaatcacg
    21481 attcatgggg gaataatcag gtaagaaaag gtgcgcggag attaccgtgt gttgcgatat
    21541 attttttagt ttcgcgtggc aatacatcag tagcaataaa acgacatatc cagaaaaata
    21601 tacactaagt gaatgatatc ttccgattta tcttaatcgt ttatggataa cggcaaaggg
    21661 cttcgttttt tcctatactt attcagcact cacaaataaa ggaacgccaa tgaaaattat
    21721 actctgggct gtattgatta ttttcctgat tgggctactg gtggtgactg gcgtatttaa
    21781 gatgatattt taaaaatcag atatgacata taagttgtca ggtcaggaac tagatatttt
    21841 gtgtacttga tttgcaaaca gctccggt
//
LOCUS       DAZFTN010000079        21725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFTN010000079 DAZFTN010000000
VERSION     DAZFTN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            690..4502
                     /locus_tag="ACHKV2_003330"
     CDS             690..4502
                     /locus_tag="ACHKV2_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO6941161.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4591..6210
                     /locus_tag="ACHKV2_003331"
     CDS             4591..6210
                     /locus_tag="ACHKV2_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6941162.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            6226..6594
                     /gene="acpS"
                     /locus_tag="ACHKV2_003332"
     CDS             6226..6594
                     /gene="acpS"
                     /locus_tag="ACHKV2_003332"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6941163.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6608..7369
                     /locus_tag="ACHKV2_003333"
     CDS             6608..7369
                     /locus_tag="ACHKV2_003333"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO6941164.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTTVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7373..7921
                     /locus_tag="ACHKV2_003334"
     CDS             7373..7921
                     /locus_tag="ACHKV2_003334"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO6941165.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7943..8224
                     /locus_tag="ACHKV2_003335"
     CDS             7943..8224
                     /locus_tag="ACHKV2_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6941166.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            8260..9420
                     /locus_tag="ACHKV2_003336"
     CDS             8260..9420
                     /locus_tag="ACHKV2_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO6941167.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9494..12052
                     /locus_tag="ACHKV2_003337"
     CDS             9494..12052
                     /locus_tag="ACHKV2_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO6941168.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            12057..13280
                     /locus_tag="ACHKV2_003338"
     CDS             12057..13280
                     /locus_tag="ACHKV2_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO6941169.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            13277..14230
                     /locus_tag="ACHKV2_003339"
     CDS             13277..14230
                     /locus_tag="ACHKV2_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6941170.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            14241..14948
                     /locus_tag="ACHKV2_003340"
     CDS             14241..14948
                     /locus_tag="ACHKV2_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6941171.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14932..15588
                     /locus_tag="ACHKV2_003341"
     CDS             14932..15588
                     /locus_tag="ACHKV2_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941172.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15589..16899
                     /locus_tag="ACHKV2_003342"
     CDS             15589..16899
                     /locus_tag="ACHKV2_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6941173.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16908..17696
                     /locus_tag="ACHKV2_003343"
     CDS             16908..17696
                     /locus_tag="ACHKV2_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO6941174.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17693..19081
                     /locus_tag="ACHKV2_003344"
     CDS             17693..19081
                     /locus_tag="ACHKV2_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941175.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            19095..20036
                     /locus_tag="ACHKV2_003345"
     CDS             19095..20036
                     /locus_tag="ACHKV2_003345"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6941176.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            20033..21019
                     /gene="ycdU"
                     /locus_tag="ACHKV2_003346"
     CDS             20033..21019
                     /gene="ycdU"
                     /locus_tag="ACHKV2_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO6941177.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21386..>21725
                     /locus_tag="ACHKV2_003347"
     CDS             21386..>21725
                     /locus_tag="ACHKV2_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO6941178.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELS"
BASE COUNT         5669 a         4817 c         5662 g         5577 t
ORIGIN      
        1 tgattgctat tagatgggaa tgaaagcttg ccaaaccgtt ttgctcttct ttctcccttt
       61 caacgataag ccaggcataa tgtggctgct taagccaatt gtcgcaaagc caaagctctc
      121 tatatagcta acctcatggt taatccgatc tcagtaatac catgagcaag aactctttcc
      181 tttttatata actcttaagg gttacatgcg tgaatgcaaa tggtaatata atgaaatgtc
      241 gcctgcccgt aatgaccata aagaatctac cccgacgtaa aagatataga gaataagatg
      301 cagtaatgat gatgctttgt taatcaaata ataagattta aattaattgt aactcaccaa
      361 ctttgttgtt ttattcggat gtctgaaatg atgtcgtaca gaaaactaca tatcatttca
      421 gacgtcctct aaattggatt tttttcaccc aaggagcaag gatgggggac ttttaaatta
      481 ttgtgtttta gtaagttagg ttcttcgccg ttcgatgaaa aaacaataac tcttagttgt
      541 ttttttgagt ccttatttca gcttattttt cgtgattttt tattgcgcca ggtgatagga
      601 tttgccgcaa tcatttttgc tgatattccg tgagtcatca aaagtgatct gcatgtccaa
      661 aatgaatagc atcaataagg aaaacgttag tggcgatgat aaatttaagt aaggaagcaa
      721 cggtggggaa agcattaacc cctattgcta tacttatgat gttgtctttt cctgtagctt
      781 ctcaagcggc gggattagtc ataaaaaatg gaacggtata taacgccaat ggtgtgccag
      841 tcgttgacat caacaaacct aacggtagcg gtttatctca taatatctgg gataacctaa
      901 acgttgataa aaatggtgtc gttttcaata atagcgctaa tgaatccagt acttcacttg
      961 ccggaaatat tcagggaaac agtaatctga cctccgggtc ggcgaaggtg atcctgaatg
     1021 aggttacttc caaaaatcct tcaaccatta atgggatgat ggaagttgca ggggataaag
     1081 cggatctgat tattgccaac ccgaatggta ttactgtaaa cggtggcggt tcaatcaata
     1141 caggtaaact taccttaacc accgggacgc cggatatcca ggatgacaag ctggccggtt
     1201 actccgtgaa cggcggtacc attacgctcg gtaaactgga taacgccagc ccgacagaaa
     1261 ttctgtcccg taacgtggta gttaacggca aagtgtctgc cgatgagctg aacgttgttg
     1321 ctggcaataa ctatgttaat gccgcaggcc aggtgaccgg tagcgtatcc gccacggggt
     1381 cccgtaacgg ttacagcgta gatgttgcca aactgggcgg aatgtatgcg aacaaaatca
     1441 gtctggtcag caccgagaaa ggtgtggggg ttcgcaacct cggcgttatt gctgggggtg
     1501 ttaatggtgt cagcatcgat tccaaaggta acctgttaaa cagtaacgcc cagattcagt
     1561 ctgcaagcac gatcaacctg acaacaaatg gtactctgga taacaccacc ggtacggtga
     1621 catctgtagg cactatctcg cttaatacca acaagaatac tatcgtgaat acccgtgcgg
     1681 gtaacatctc tacgatgggc gatatctacg ttaacagcgg tacgattgac aatactaacg
     1741 gcaagcttgc ggctgcagga atgctggcgg ttgataccaa taacgccacg ctgattaact
     1801 ctggtaaagg gagttctgtc gggattgaag cggggctcgt ggcgctgaaa accggaacgc
     1861 tcaacaacag caatggtcag attcgcggtg gctatgtggg tcttgaatcc gctgcgctga
     1921 ataacaacaa cggtgatatc cagaccaccg gcgatatcgc cattatcagt aacggtaatg
     1981 tggataacaa caaaggtctg atccgttcgt ccaccgggca tatcgttatt ggcgcggcag
     2041 gtagcgtaaa taatggttca accaaaaccg ccgataccgg cagttctgac tctctgggca
     2101 ttattgcaga taccggcgta gaaattggtg cgaacaacat caataacaac ggcggacaga
     2161 ttgcgtctaa tggcaacgtc tccctgtcaa gttacagcac gatcgacgac tatgcgggca
     2221 aaattctgtc caacagcaaa gtgattatca agggaagctc tctgcgtaac gataccgggg
     2281 ggatcagcgg taagcagggt attgaagtcg ccgttggcgg cagcctgacc aataatattg
     2341 gcgtgatcag ctctgaagag ggtgatatct ccctgttagc caactccgtg gataaccacg
     2401 gcggcttcat gatggggcag aacatcacga tggagtcgat gtctggcgtc aataacaaca
     2461 cagcgctgat cgtggccagc aaaaaactga agataaatgc gcgcggcagt atcgaaaacc
     2521 gcgatggcaa taacttcggt aatgcttatg gtctgtactt cggcatgcct cagcaaacgg
     2581 gtggaatggt cggcaaggaa ggcatcgagc tttccgggca gaacatctat aacaacaaca
     2641 gccgtcttat cgctgaggat ggtcctctga ctctgcaggc gcagaacacg ttcgacaaca
     2701 cgcgtgctct ggtcaccagc ggggcggatg catctattca ggttggcgga acgtattaca
     2761 acaactacgc taccacctgg agtgcgggca acctggatat cgacgcgacc acgctgcaaa
     2821 acagcagcag cggtacgatg atcgataaca atgcgaccgg gttcatagca tctgataaaa
     2881 acctgtcact ggaagtggtg aatagcctta ccaactacgg ctggatcagc ggtaaaggcg
     2941 atgttgatgt cacggtgaat aacggcaacc tgtataaccg caataccatt gcggctgaaa
     3001 aggggctgga tattgccgcg ttgaacggta ttgaaaactg gaaggatatt tctgctggcg
     3061 gcgacctgac gatgaacacc aatcgccatg tgaccaacaa ctccaacagc aatatggtgg
     3121 ggcagaatat tgttattaac gcggttaacg atatcaacaa ccgtggcaac attgtcagtg
     3181 acgctgacct gaacgtgacg accaaaggca acctgtataa ctatctctat atggtagggt
     3241 atggggatat cgcattgtcg gcaaatagcg tggcgaacaa taacgcgacc atcgaagcga
     3301 caggcgatct gattatcgat tcgaagggta acgtgggtaa caaccgcggt aatctgcatg
     3361 cgttgaacgg cgtgttgtct gttaaaggca acaatctgaa caacgataac ggtgaaattc
     3421 gtggttatgg cgatgtcacg ctggcactga cgggcaacta cgacagctat aagggttcgc
     3481 tgacctctga aacgggcgac gtgactctga cggcgaacat tgtagacaac gcctatggtt
     3541 tgattgccgg tgagaatgtt tctgtcgatg ctaaatcgac gatttacaac aacactgcgc
     3601 tgatcgcggc gaataaaaag ctggttatta acgctggcgg caacctcgaa aaccgcgacg
     3661 ggaataactt cctgcgtaat aacggcgcgc tgtttggaat taccgacaac gttggcggca
     3721 tcgtaggtaa agaaggtgtc acgctttctg ctcagaacgt ctacaacaat aacagcagca
     3781 tcatcgctga aaatggtccg cttaatctgc tgtccagggg aacgctggat aatacccgcg
     3841 cgcttcttag cagtggggct gatgccatca tccgtgcggc agggacgttc tacaacaact
     3901 atgccaccac gtacagcgcc ggtaatctcg acgtttatgc ggcgtcgttg aacaacgcca
     3961 gcgatggtcg cctggaagac aataccgcca cgggcgtgat tgcgtctgac aaaaacctgg
     4021 atctgagcgt tgataacagt gtcactaact atggttggat cagcggtaaa ggagatgtgc
     4081 atttcaatgt tctgaaaggc acgctgtata accgtaatgc catcgcggcg gacaacgcgc
     4141 tgaccattaa tgccctgaac ggtgttgaga actttaaaga cattgtggcg ggtactgcgc
     4201 tgactattga tacgcagaag tatgttacca acaacagcaa cagtaatatg ttgggacaaa
     4261 ccatcgcgat caatgccgtg aatgacatta ataaccgtgg aaatattgtg ggtgattatt
     4321 ctctgggtgt taaaaccacc ggtaatattt ataactacct caatatgctg agttatggtg
     4381 tcgctggcgt atcggcaaat aaggttacga atagcggtaa agacgctgtt ctcggtggct
     4441 tctacggttt agcgttagaa gcaaacgaaa ctgataacac cggtactatt gtcggcatgt
     4501 aagatgtaac caaagacagc agtggctttt aatattaatg cgtcatgtca cgcaggggtt
     4561 gctgctgtct ttatttagtt tgagaagata atgcaattac gatttaattt tggtttgctg
     4621 ctcattcttt gttgttttca ggttgcagcg aaagacagtc ttgataattt gatcaagcag
     4681 caaaaaaatg cagatgaaag cacgttgcgc gcgcataaat tagaaaaaaa agatgtttac
     4741 tcaaatgcac aacgtaatgc gtttactctg ggtgatctgc cgcaggaaaa gaattgcttt
     4801 atcattaatc ggattgaact ggaagataac tttctgggtg gtcagttgat ttctgaagtg
     4861 caaaaagcga ttgccggacg ttgtgtcggt agtgctggtg tgcaaaaaat tgcagctgcc
     4921 ttgcaggatt attatattaa tgcgggatat atcacaacgc gaattagtat cccttcgcag
     4981 gatatttcta aaggcaagct acgtttccag gttaatgccg ggaagataga aaaaacgatt
     5041 gtcgaaggta atgatatcag tgaatggatg ttaccttttc agcgtaacga tattctcaat
     5101 attcgtgata ttgaacaggg gctggaaaat cttcagcgag tacctgacgt tgatgtgaaa
     5161 attaatattg aaccgggaac acgggatggt tacagcatcg ttcatattga tacacaccgg
     5221 gggaaaaact ggagcgttcg ggcaagttac aacaattggg gcgatgaaga aacgggccgt
     5281 taccagacaa cctcggtggg ttatctgttt aatccagcga aaatggggga tctgttttat
     5341 ctcgccggta cgcgtagtac taccggacag tatgaaaacg tcagtagtta ttattccatt
     5401 ccggtggggt actgggagta cggctttttc tatagcaaaa gtaaatcgca gcaggtgatt
     5461 ccgctttcgt acatctcgct ggattatgtt ggcatcagcg aatacttgag cgcaaaagcg
     5521 acgcataccg tttatcgcga taaaacaaaa aagttcgccg gtagtgcaga actcattcga
     5581 cgaaagtcga attacacgct taatggtgag gagctgacat tacaggcgcg ggatatggga
     5641 aatatcaagc tggggctaaa ttataagcaa cagttacccg gcgcgttctg ggattcaacc
     5701 ctctcgtggc aacgcttttt aacctggttc ggtggtgaaa aaacgccgga tatgcaatat
     5761 ggcgacgtta gcccggtaag caatatcttt aattttgagg gtaactatac ccgacaaata
     5821 gagaatggat attacaatac cgcttttttt gcccagtacg caccgcgtga actgactttg
     5881 caagataaaa ttacagttgg cgatcgttgg agtattcgcg ggtttgaaaa tagcgtaggt
     5941 ttgtcaggga atgatggttt ttatataaaa aatacgctgg catttccttt gcctggaatg
     6001 aaagctaatt attatgccgg tcttgatttt gggcaagtgt atcaggacgc ttcttatggc
     6061 gatgaatcac tggtgggctc ggcagttggt attgatggaa acattaaaag tcttgagtat
     6121 aacttttccg tttctacccc attaaagtat ccggcgacat tagatattga tcgggtgaat
     6181 gtaaatttta atttttcgta ccaaatgtaa aggataacga gactcatgag aatagggaca
     6241 gatattgttg aaattgccag aataaccaaa gccattatta atggagagat ggctttttat
     6301 gagcgtgttt atacttctca tgagcgctta ctcattaacc ctgcagaacc tgattatgaa
     6361 cgggcctctg gattctgggc ggcgaaagaa gcgttagtta aagcgattgg ttgtggtttt
     6421 cgtgatggcg tacgtttcca ggatattgaa ataaagcatg atgcagccaa agcaccttat
     6481 ttcgttattt ccggaagact acaagaaatt tttatcgggc aggggttgca aaataactcc
     6541 ctgagtattt cgcattgccg tacccacgcc attgcgactg tcatcgttta ttaactcaac
     6601 atatgtgatg aatatgtaca aatataatga tttaaatgaa aaaaccgtac tggtgacagg
     6661 agccagtggt gatattggac tgggtatttg cgaaaaatat ctggagcaga actgcgatgt
     6721 ttatgcgctc tataaatcca acgacgtgca attaacggcg ctgaaagcaa gccatcctgc
     6781 aggcgataaa ctgcatattg tgcagtgcga tcttgcctgc ccccagagcg tgagcgcatt
     6841 gtgcgaacag atagagcgcc aagctggcaa gattgatgtg ctggtaaata acgcgggcat
     6901 cgttaaagac agcctgtttg cctcgatgag ttatgaggat ttcacccagg ttatcgaaac
     6961 caacatgttc agcattttcc gtctgacgaa agacgcgctg atgctgcttc gtgcggcaga
     7021 aaatcccgcc attattaacg ttgcctctat tgcagcgctg atcccaagcg tcggtcaggc
     7081 gaattacagc gcgtcaaaag gggcgatcct aggcttcacc cgcacgctgg cagcagaaat
     7141 ggcaccctgg ggcgtgcgcg tcaacgcggt cgcaccggga atgattgaat cgaaaatggt
     7201 gaaaaaagta tcccgcgcag tggtccgtgc ggtaaccagt accatcccgt tacgccgtct
     7261 gggtaaatgt gaagaagtgg cgaacaccac cgtcttctta agttcctctg cctccagcta
     7321 cattgtcggc cagaccatcg ttatcgatgg cggcctggtc atgcggtgat ttatgtctaa
     7381 atacacgatt ccttcgaaaa tcttcctcga aatgggcggc tggcgtcagc cgctgctgat
     7441 ggtcgataaa attgcagatt ataaatacgg tgaaaatggc tttgtcagtg tcgtgaaaca
     7501 cgtcacctat aacgaaccgt atctactggg gcatttcccg gaagatccga ttatgcccgg
     7561 ggtgattatt tcggaaatct tcggccaggc cagcgagtat ctctctttcc tgaccgacat
     7621 ttgcgatatc tggcgcgaac gttttgagga agagctgaag tcgttacgtg atattcacgc
     7681 acgcattcac cgccctgaaa tgctggagat tatccgtacc cggcgcagcc aggtacgcgg
     7741 cgtgctggcg gctcagaacc tgaaatttaa agatatcgcc tatccgggtg attctattga
     7801 tgttgtcagc aaattggctt tttcggatgc cagtggcttt aaacactatt ccgtgaccgc
     7861 gtacgtcggt aaaaagctga ttagtcaggg caccattatc aatttccgtg aaaccaagta
     7921 atccaataag agagtaaata ttatgtctac catgaatgac gctattgaac agcgtaaaga
     7981 agttctgatg accattaaaa ctgagatcat ccagcgactg aatattcaac gcgatgaagc
     8041 gcaaattgat gatgatactg cgctgtttgg taatggtttt aaactggact ccgtcgatgc
     8101 aacggaaatt gtcgttctgc tggataaagt atttgatatc aagatcagcg aaagtgacga
     8161 cccgtcctat atgcgcagta tcaatagtct ggccaccttc gttattcaaa agaaaaacga
     8221 ttaactactt attaaagaat taattatcag gacacgttta tgaaatctct tgctgaattt
     8281 cacttgaaaa ataatgcggt aatgggggta tataacaacc gtaccctacc ttcttcttat
     8341 cacgatgcta tgaccgaata taaagccgtt cgtgaaaacg cgctgctggt ggactattct
     8401 cacctctcta ttgttagcgt catgggcgat gatgcctggg cgctgatcaa ccagctggtt
     8461 tccgcagatg tctccatcat tcgtgatgag caggcaatct actcactggt gttgaatgaa
     8521 gagggcacca ttcgcggaga cgtctatgtg ctgtgcagca ttgacggtta ttacctgctt
     8581 tcagaagata tttcggctgc cgaattgatc gccagcatga ataccattct cgaaaaagcg
     8641 gaagaactgg atattcaatc gatgcctgaa attcaggata tgcgggaaaa caactggggg
     8701 gcaatcctgc tcgaaggccc atactcctgg gagatcatgt ctgaaattca tggctttgac
     8761 gtgattggcc tgccttacta cgaatacatg aataccgagg aggatctgct gctcttccgc
     8821 tgtggtaaac acggtgagta tgcgtacatg accatcggtg agcaggcgaa actggcggag
     8881 cagtgggaaa aactgttaac cgttggcgag aaatacctga tgcagaccgg cggcctggat
     8941 tatcaaaaaa tcgtacgcct cgaaaatccg tgctgggatg ccagcctctg ggaaggtcag
     9001 gcggtgaatc cggttcagtt gcagatgcag tgggcggtgc agtacgacaa agatgatttt
     9061 attggcaagg acgcggtgac agagctatcc caggagtata ccggtaataa actcatcggc
     9121 atgatcgcac aggaagaatg cgaaggcatt gaggctggcg atcgcgtgct ggtggaaggt
     9181 caagacgtgg gctatgtcgt gaaggcactc ttttccccag cgttgcagcg tttcattgcc
     9241 ctgaccctgc tggaaaagga ttacgcctgg tctgacatca gcggctacga aattcaaact
     9301 gctcacggaa ttattccggc gcaatcaaaa tgtatgccgt ttatttataa cctgagcatg
     9361 ttggttagcc caactgagca cagttatatc gacgcgtcaa aaaataaaag cgcagcgtaa
     9421 tgaatatcgc cgcgtgacta tagcttacgc ggcttaaatt aaccccaatt ggcgggattg
     9481 ccaggtatta ttcatgaaaa cgaacaggaa aagagttgtt attaccgggc tggggatttt
     9541 gtcctcactg gcggaaaata ttcaggattt ccgggaagca ttattaaata aaaaaaatgg
     9601 cgttaaggat agcgaacgct ttgcaaaatg gtttgaaaat gcgcgtgcgg cagaagtgtt
     9661 gcatgacatt gaatactccg atctgaacga agatgttatc gattcactgg ataacgcagc
     9721 gctctgggcc tacaaagtgg gtaaagatgc gctgagtcag gccggcctga tggatagccg
     9781 tgacaccctc aaagaaacag gcatgatcgt cggtgtttcg tccgcgggaa cagaggcatt
     9841 tttgccgctg ttcgaacagc gcatggaaga tttctcgctg cgtaaagcac tcttttccgg
     9901 cgcattctca tcctgttgtt ccagcgtttc aacgctgctg ggcttacagg gtggggttga
     9961 actggtggcg accgcgtgta ccgccagccc gaacgccgtg gggatggcct ttgactacat
    10021 ccagaatggt aaaagcaaaa ccatgctggc agtgggcact gagcccatct atctgccgac
    10081 ctttgccgga ttctacgcac tgaatgtcat gcatccagac acttgtacgc cgttctcggg
    10141 tcagtccggt atgtcgattg gtgaaggcgc tggcgctatc gtgctggaag agtatgagca
    10201 cgccgtagca cgcggggcga ccatttatgg tgagatcctc tcttacgcca cttcctgcga
    10261 tgctttccat gaaaccgggc cagatccgcg tgccagcgga gcggtgcagg tcatgaacaa
    10321 ggcaatggca aatgcgggcg taaccccgga gcaaatcgat tacgtcaacg cgcacggtac
    10381 gggtaccgaa gccaatgacc gtattgaaac gctggcaatg aaaaaggtct tcgctaacca
    10441 cgataaactg ctggtgagct ccaccaaatc ttactttggc cacaacattg gcgcagcggg
    10501 gattgtggaa ctgattgcct gtctggcaac gcttccggat aaccgcgtac tgccgacgct
    10561 gaatttcagc catgcacgcc cgaattgcga tctggactat gtgccgaatg agtttcgcga
    10621 tcgcaaagtg aacatcttca tgaagaataa ctatgccttc ggcggcaaca actgctgcat
    10681 gatcctgagc atggagcccg cgtccattcc ggtcagtacc tatgattcga agcgtgtggc
    10741 tatctcgggc gtcggtgcag tatccgcaat aggccacacg ctcaaccagg ttctggaaaa
    10801 catctgggcg caggaccacc acgttgaact gggtagcgta accttcccgg acgatacact
    10861 ggaagaagcc aaagagctgc tggccgtgct ggatgaatca aaccagtttg ccgagctgtt
    10921 tgatgaagag tacagcttca gcgaagctac gcttccggaa acggaaagca actttaaaac
    10981 gttccaggtt tccgggctgg agccgcgtaa acacctgcgt cgtttcgatc agcgtaaagc
    11041 gacccgtggc ggcacgtttg ccctgatcgc actcaccgaa gcgctggaac aggcgaagcg
    11101 taaaatcaaa cgcgacggcg atgagttggg catgatcatg gggatgtccc gcggcccgca
    11161 ggaaaccacc tacaaatatc tgcaaagtct gaaacccgat ccacgcaaag tacgtacatc
    11221 ggaatttcct ggctcgctga tgaatgcgat cccaacgttc tgcgggatct ctgaagggat
    11281 caaagggtat accactacgc ttgcgacagg ggaaaactcg gcgctgggcg cgctgaccta
    11341 cggttatgaa atcgtccggc agaacctgca accgcaggta ttggttggcg gcgcggatga
    11401 gtacttcccg tcgatgtcgc tgtatatgga tgcggtgaca caaaagatcc acatgacggc
    11461 tgaggcgagc gactatcaga tttacggtaa agatccgcaa ggttatgttc cgggcgaagg
    11521 ggcttgcatg ctgctgctgg aagatccgca gttggctgtg gcgcgtggcg ctgaggttct
    11581 cgcggaagtg gtgggctacg gcaagtcatg cagcaatacc tacttcgacg cctcattgtt
    11641 ggaagaaaaa tccgcgtcga tggcgctggc cattgaacgc gcccttacgc atgcgggcat
    11701 ctctgctggc gatattgatt tggtatgtgg taccagcaac ggtagcgatg accattctcg
    11761 tatcgaaatt gacgcgattt acaacacctt cgccgcgcaa aacccggcgg tgccggtagt
    11821 gaactataac gcttgctttg gttttgtcgc ctctgccgct ggtttgctta acctcgccgt
    11881 gattactgac tgcattaaga agcaggcagt acctgcgatt ccaaatacgg tggaattctt
    11941 tgatgagcgt gtgaattttg tacgccagtc gatgaaactt gcgctgaacc atgtcctgct
    12001 tgttggcgca acggaaggcg gcaactacta cgcgttcgtc attaagggat aagactgtgg
    12061 acaatgccgc tattatttcg ggttacagcg tgtggatgcc gtatgccgaa gactgttcgc
    12121 agttaattga caatctgaaa caggggaaac gcgttgcgcg tacaccctgg tttacctcca
    12181 acgaggaagc cattaaatgc gggtttaagg gtaatccgtc agtggcgacg ttgaaacagg
    12241 tggacgatag cgcattggac ctgctgtctc agttgatcga cgaagccctg gaacaggcaa
    12301 tgctggataa gcattgcctg gccgggcgca acgttcgcgt ttatctgacc ggtattgggc
    12361 cgcgtatcga cgggctggat tacaaatctt tctataatta caacgatgta gaagatatca
    12421 acttaacgca atccatcaca aatctgcatg cctcaaagat gtcgcaggac acgatttcaa
    12481 gccatcttgc gcgcaaatat cgcttgcagt atttgccgcc taacatgaac tgcaccagta
    12541 actcgtcact gactgcggtg catctggcga cgcagggcat tgagcagggc gggattgatc
    12601 tcgcgattgt gctgaactgt tcgaaaatta aaacccagga catctggttc ctggaaacgc
    12661 aatcgatgct ggacagcgaa caagtgcagc cgtttggtga aaacagtaaa ggtgtggcgt
    12721 tcgcagaagg ttttagcgcg ctcctactgg aaagcgctca tcaccgccgg gcgcgtcaac
    12781 aaagtgaggg cgtaagggtg caaacgactt acacccaaat cagcgcgggc cgcagcaacg
    12841 atgcctcctg gcttagcact aacgtgcaca aggtcatgca ggccgcgatg aagcaggctg
    12901 aaattgcgct ggatgatctt gcagccattc ttccacacgg caacggttcg gcagtgagcg
    12961 ataacgcgga ggccaaagcc atcgccatgt tcgcagggga gcgacagatc cccgttctcg
    13021 cctataaagg gcagatcggc tataccgcaa ccggatctgg tgttgtcgat ctgatcattg
    13081 gccaccattc gttgacgcat catcaactga tcgcacctgt tggcaacgac gtcattatcg
    13141 acagcatggc ttcgctggta cttacggacg gcagcgtgac aaaccatagc aaacgccatt
    13201 tgctgaaggt tggtgtgggt gtcgatggtt cagttattgg cgttgtcatg acaaatatgc
    13261 aagcggggcg cgcgaaatga cggaaaagag tatctacctg tcttcctggg cgcttacgga
    13321 accggacgac attttttatt tttatcagcc gacctggctc aacgcctggg aagcacatat
    13381 gtacgaaagg ggattatacg gcacccgtgc taatggctgg ggactgaagc gctttggcgt
    13441 ggagaacgat atttatgcac cagaacggat ccgtaatcca caatatatgc cttaccttga
    13501 ggcgatggag caggtgaccg attctgcatt acaacaaatg ccgactaaag aacgcatgaa
    13561 gttaatgaaa tcgcgcacgg catttattta tgccgattcc tggggcgaat ccggttcgtt
    13621 tgaaaaaatt tccagtgcgc tgcatatcgc cactattgat acgcttccca agaatttact
    13681 taagaagttt tcgataacgg aaccgacttg caagattcgc ggcgaaaaac aggcattcgt
    13741 tcaggcgctg cgtatggcgc aggattatct cgcctgggat gtattcgata acgtggtgat
    13801 ttgtgctgcc tatcgggcaa ttccggtgct ggtattctct gaagaagatg tgggtaaacc
    13861 gcctaagcgt ttatttcccc gtcgtgctga tgaggtgaat ttatctgttg aacgagtggg
    13921 ttgttttatt ctcagccagc gtgagagcgc catcaggctg caatgcgggg aatatcattc
    13981 cggcactgcg ccacagcatg ctacggatat tgatgtcatg gccttcagcg aacggcaaca
    14041 aatgccgtcc cggcctgctg ctggcgttat cgacctggtg gcccattacg gtgaaagcgg
    14101 ttgtctgacg cccgcgttga gtatggatta tttaacccgt catattcagc ccggtggaaa
    14161 aatgcgtacg gtcattgccg ataaacagtt tggttatcac tactttgacc ttgaatacct
    14221 tgaaggataa cgacagtcgt atgcaatcta ctattaaatc gttgcccgct atttcgctta
    14281 gcaatattta taagtgttat ggcgctgggg caggaaaggt tgatgcgctt aaaagcatta
    14341 atcttgatat caataaaggc gaatttctcg cactgtgtgg gccgtcaggc agtggtaaaa
    14401 gtactctgtt gaatattctc tcgggtattg ataaaccgac cagcggaacg gtgatatttt
    14461 taaataaatt actgaaccaa ctgccagaag agcagttggc tgtgattcgt ggaaaacatt
    14521 tgggttttat ttttcagttt tttaacctta tgccggtatt aaatgttttc gataacgttt
    14581 atttcccact ggtgcttaat ggtcagttca gtaaaaaaga agccactgaa cggacgctgc
    14641 attatctcga tagtgttgga ttaagcggtt ttggcgatcg taaacctggg caactatcgg
    14701 gcggtcaaca acagcgtgtc gccatcgcca gggcactggc ccacgaaccg atggtggtca
    14761 ttgccgatga accgacggga aatttagacc tggcaaccgg cgaggctatt ttggacctgt
    14821 tgttgacgat taatcagcag accggcacca catttgttat ttccacgcac tccagcgaat
    14881 taaaagcgcg ggcacggcga gtggttgaaa ttcaggatgg agtattgatt catgactcag
    14941 atgcataaag gatttttgct catcggcgtc tttttgtacg cggcgtttgt ggctccgaag
    15001 atttacgcgg caacacccga cgctgttaac caggttctgc aacaggggct cccggcgcag
    15061 cctgccgcgc tggcgacgct gaccgaggcg ttaaagaaag agtatgaagc caggcacaat
    15121 gtggtggcgc tggtttttta cgcctatggc ttactgcgtc aagcggacgg ttatgcggca
    15181 acaaacgatt ttatccatgc ctcagaatac gccaaaagcg gctttttctg gctggatgaa
    15241 gcggtggatt tgcaggagaa aaatcaacgc gtacggtatt tacgcgcccg cgtagatgcc
    15301 tatttacccg cagactcagg gcgttgcgtg gtgacagtca aagacaccga acacatgctg
    15361 gctgacccgg cgatatggac gacaacgatc cgcgaccata ttctggcgat gcgttatcgc
    15421 gcattacgtc actgcaagga caccacccgc gcgaatgcgt tgttggcgca aattaaaggg
    15481 caaaacgccg cgcttgcgca gtcgctgacg caggatttta atgttgtacc agaatgggat
    15541 agtgaagagt taacccaggt actgctgccg ctgatgaaag gtgagtaaat gtccggggaa
    15601 attttagctg ttaattgcgt tctgctgctg attctgtttt ttgtgtttta tcagcttctg
    15661 ttttattcac gagatgtcaa atttgcattt ttgaatctct ttcgccacaa gcgtcgctct
    15721 ttttctacca taaccgctat tgtattgggt ggcgtggcaa tttttttata tggcggattt
    15781 attgattact ctttctggat cctgaaagag cagacgattc gtaccaatat tggtcatgta
    15841 caaatttaca atcagcacta ttttgaaacg tcgaataaaa ataagagcct gattgccgat
    15901 tatgcggcgt taaagaaagc tattttgtcg gacccgacgc tggcggcgga tatttccacg
    15961 ctctccgggc aactggagtt tactggcgta atttcacatt acgagagcga aacctccagc
    16021 tatttctccg cgctgggggt cgagccgtta cccgctttaa agctgggttc gttcgataag
    16081 attatcgcgg ggagcgatct ttcccgggta aaaggtgatg aaatcaccct gggcagcggt
    16141 ctggcgaaaa cgttgaatgc gaaatatgac gactggctgg atgtgatggt ggtcaacacg
    16201 gcgggcgggc agggcgcatt gtcgctaaaa atgcgtggca tttttgagtc aggcattaaa
    16261 gattatgacg atgtcgcgat gaagatcccg ctggacaccg cgcagcgcat gatgggtacg
    16321 gatggcgtca gcaaagtgct tattctgctt aaagaggatg acaccgcggc gtttagcgca
    16381 aagctgcgtc aatttatcga acgcaatcat ttgccgctgg tggtcaaaga ctggcgtgat
    16441 gtttcactct tttatcaaca ggttgaaggg ttgctatcgg ggatctattt cttcatcaag
    16501 ctgattgttg cgctgattgt catcttcatg atcggcaact cgatggcaat gaacattgtt
    16561 gagcgcaccc gtgaaattac cactctgcgt gccataggcc tgaagccgct tcacgtcacg
    16621 cgcttgtttc tgacagaagg catttttatt ggtgtgattg gcgcagtcgg cagcctgctg
    16681 gtggggggcg tgttagcctg gattattaat ctttatggta ttgcgatgcc gccatcgccg
    16741 ggacaaacca tcggctatac cgcctttata aaaaccagta acccggaatt gatttgggtg
    16801 acggtggtat tacctatttt gacagcaaca ggtgcctctg tgttaccggc tttacgtgct
    16861 tcccggctga atatttctga cgcatttaaa ttctcatagg gctaattatg aatatggctt
    16921 tttacggcaa gtggtttgct tgcctgtggc tcgctacgtc gtgcgttcag gcggcgagca
    16981 cagataataa ggcgctggag ataattcgtc gcgctgacga aattcgttcg ccgaataaac
    17041 cgtttcgtta cacgctgacg gtaacggaat ataaagcggg cgcaacccag ccagagaata
    17101 aacaggtcct cgatatttcc atgcgcttta tgaaaccgca gggtaatgaa aaagcggatg
    17161 cacgctcgct ggtgcgtttt atttatccgc cgcgagataa aggaaaaatc atgctttccg
    17221 actggtacga tttatggttt tacacgccgg aactgcgacg tcctatgcct atttcacggc
    17281 aacaaagatt aattgggcaa atctctaatg gcgatgtcat tgtcactaac tttgaatatg
    17341 cctatgactc gaccctgatg ggggaagtca cgtgtgctga aaagcagtgt tacaagctgg
    17401 cgctggtgcg taaatcggcg gatatcacct ggccgaaggt tatctattac gttgaaaaag
    17461 acggtgataa tcgcccgtgg aaggcggcct attattcgca ggatgaccag ttaataaaag
    17521 aagtgttgta tcaggatttc cagccggtgt tggggaaaac gcgacccatg aaaataaccg
    17581 ttaccgatgt tcgtcacggc aataactatt cagtgatgga gtacagcgat gttcgtctgg
    17641 aatcgctgcc ggagtttcat ttcaccaaag agtatattca gcgaggtgcg aaatgaaacg
    17701 tacgctgaag atctcttcgt tgctgtgtgt tgcgctgccg ttgacggtac aggcggattg
    17761 cctgtcaggt gacgaggtgg cacaaaatag cgatgtgacg ctttacgagc aggtgtctta
    17821 tgtcaataaa caggcttcgg cctggcagat tgcaggcaaa aatccctaca cccgtcacaa
    17881 cggttatcag gaggcgggga ttggcatcaa tagcggttgt tcgataatcg acaatacgct
    17941 ggaccttaag ctcaatctgt atggaataaa cgaatatgcg cttaaacccg cagggaaatt
    18001 cgaaacggat gatagccgca cgcgtgcgtt aattaaccgc ctcagtctgg tctatagcgc
    18061 ctcggacagc gttcaatttg aagcaggcaa atttgccgcg ccgtcaggaa tgttcttttt
    18121 gcgttcgcct tctgatttgc aaacgcatta ttacaccgga ttccagtcga cgcgtttgca
    18181 cgatcccaaa atgacatcgg cgtatcaggc gtcatcgtgg ggcgcaaaaa tgagcgtgga
    18241 tacacgcgat tatgcgtttt cggcgagtgt cataccgaag ttagccacca tcgacaaacg
    18301 ctatgtgacg tctggcaact ggtcggctaa tcagcaggga aacagcgacg aagcctatct
    18361 gctgagcttc agcgatcatc gctttggtga gcacacgccg acagtcaatg tgcggctcgg
    18421 accatcgccg tcactcgcgc tgtctgacag ttatcactac accccgcagc ttacgctgaa
    18481 tgtcgacgca gcctaccacc gctcccagca atggcggcat ctttcccacc gtgagacggc
    18541 gcaggtcgag gaatatcagt ttccggattc gctctatgaa acgaaagatg aaagcggcgt
    18601 agagctggcg ctgggtgggc agtacaccag tgataatttc agcgtgtttg gcgttgagta
    18661 ttactttcag agcgagggtt attcgcgcgc tgaacagcgt cagcagcgcg agttaattga
    18721 cttcctcaat accacaaccg gctatgcgcc actggatcag gcatttgatt cttacaaata
    18781 cctgatggct tcagaaatca gcaataccgc caatcagggg atgctgcagg gtaagcacta
    18841 tctgaacgcc tgggccagtc tgccgctggc gggagagtcg acgctgcagc cttcgctggt
    18901 ggtgaatctc gttgatggca gcacgttgct gggcctgcat tattccacgc cgctcagcgc
    18961 tataagtaac cagcttgaag cctatgccgg tggctatagc gcgctgggaa gtcggtactc
    19021 cgaatttgcg ctttttggcg acacgttagg cctttatttg ggctttaaat actatctata
    19081 aggataaaga gatgatgaat aatgcttcac aaccgacagt actgcttttt gccggtcagg
    19141 gtaatccggt tattggtatg ggcagcgatc tttgggatct caacgccagt acaaaacata
    19201 tctgggattg tgccagcgat atttccggac tgaacctgcg tcgcctgtgt cagaaagggc
    19261 cgatgaatcg ccttattcaa acgacggtac aacaactggc ggtgaccgca attaatgtaa
    19321 cgctctatac cctgtgccgc gagcgtcttg agaatttaac ggttgtgggc tcctgcggac
    19381 acagcgttgg tgaatacagt gcgctctacg ccgctggagc tatttctctt gagacgctat
    19441 ttggcatgat tcacttcaga gccagcctga tgcatgacga aagccagaga aataaaggcg
    19501 caatgctggc ggttaaaggc atcaaccgcg aacaactgca gacgatgatt acggaatcag
    19561 gcattgcgct ggatattagc tgtgataaca cgccgcgcca gcaggttatt gggggaacgc
    19621 aggctgcgtt gaatgaattt gccacgctgc tgatggccgc tggatacgaa ccggttaaac
    19681 tcggtgtgag cggtgcctgg cacacgcgtt tgatggaaga cggcgttcag gcaatgcgcg
    19741 attatcttgc cggactggat atcgcttcgc cagagcatca ggttctgatg aacgtaacag
    19801 caaaaagcga ggtcgctccg tcaattatca aagaaaatct ctcattacac ttaactcata
    19861 cggtgaaatg gactgaatct ctcgatacat ttctgaatat gccaacctcg gtagctttcc
    19921 ttgaaataag taataagccc tatttgggga atatgttcaa cgatttcgct ggcgtggatc
    19981 agcaacgagt tatgcattgt cgtaaagcat ttagtgatgc gaaggtattc aaatgattcc
    20041 tgattattta acttttattc gctttcagga taaacgaagt ctgatataca tttatgctat
    20101 tggacttatt ctgataggct tttattggaa aaatgcagga tttacttttc catcagagga
    20161 tattggtgtc gttagtggga ttctggccct ggtgctgtat aattttattt ttgatctcaa
    20221 ggcgtactgg gcttataaat gcgtcacgaa gaatatcgat ttttcgtggt ttaagaaaaa
    20281 gcagaaccac aaaatagaat tatttcttac gcaacctctg gtggcaggat ttctgtcgtt
    20341 aatcatgttg agtgcaatga gttgggggct ataccagcgt ctaccctcgt tatatgcgct
    20401 gttcctgatt tcgttacttg ggccgttggt tatctttctg ctgtttcgga tgatccgcac
    20461 cagttatgtc aagcaggtcg ctatttcagt agcgaaaaaa gtaaaatata aaagtctgac
    20521 tcgctatgtg ctgctttcgg tgtgcatctc aacggttgtt aacctgctta ctatcagccc
    20581 gttgcgtaac agtgattctt ttgtgataga ggggcagtgg ttaacgttta aatcgataat
    20641 tgcattgctc attctttgtg gcgtagtgtt ggcgattaat ctgttttttc tgcgcttctc
    20701 caggcggtac gcttttttgg gcaggctttt tttgcaggaa atcgatctct ttttctccag
    20761 tgaaaatgca ttgtcgacct tttttgccaa gccgctttgg cttcggttat tcatattgct
    20821 ggttattgaa gtgatgtgga ttacgctggt gtcggtattg gcaacgcttg tggaatggcg
    20881 gatttggttt gaagcctatt ttttactctg ctatgtaccg tgcttaattt actatttttt
    20941 ctattgtcga ttcctctggc ataacgattt tatgatggca tgtgacatgt atttccgttg
    21001 ggggcatttt aataagtgag gaagtgatag gaagtgacca gataatacat atatgttctg
    21061 tactctcttg cgcattttga ttgttgactg agcaaccaga cagttgatgt gcacgatttc
    21121 ccctcgccca aacagacgtg ggcgggggca cccggtgtgt gcaatatacg aaaaaaaagc
    21181 ccgtactttc gtacgagctc ttctttaaat atggcggtga gggggaccgc ctgaaataaa
    21241 tctgacttaa tccaggggtg ttatataatt tcattataaa caatgtatta cactttttgt
    21301 tgtccgatat tatctaatgt attcttagtg aagcgagaga ctaatggcat actggatggc
    21361 ataccagagt gacgggaatt agcgtatggc agtattgacg gatacgaaag caagacatat
    21421 caaacctgat gacaaaccat tgccccatgg gggaattacc ggactgaccc ttcatccttc
    21481 ttcagtaaag gggcggggga aatgggtttt tcgttatgta agtccggtga cacaaaaaag
    21541 acgtaatgct ggattgggaa cttacccaga ggtcagtatt gctgaagctg cacgtactgc
    21601 ccggataatg cgagagcaac ttgctgcagg tgatgatcct ctggagatta aaaaggctga
    21661 atctgagaaa gtcgctatcc caacatttgc cgatgcagcc aggcgtgtac atgcagaact
    21721 gtctc
//
LOCUS       DAZFTN010000080        21569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFTN010000080 DAZFTN010000000
VERSION     DAZFTN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..222
                     /gene="ghoS"
                     /locus_tag="ACHKV2_003348"
     CDS             <1..222
                     /gene="ghoS"
                     /locus_tag="ACHKV2_003348"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6941179.1"
                     /translation="RLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDL
                     IRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            250..423
                     /gene="ghoT"
                     /locus_tag="ACHKV2_003349"
     CDS             250..423
                     /gene="ghoT"
                     /locus_tag="ACHKV2_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO6941180.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(542..2059)
                     /gene="lysS"
                     /locus_tag="ACHKV2_003350"
     CDS             complement(542..2059)
                     /gene="lysS"
                     /locus_tag="ACHKV2_003350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6941181.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2296..3753)
                     /gene="dtpC"
                     /locus_tag="ACHKV2_003351"
     CDS             complement(2296..3753)
                     /gene="dtpC"
                     /locus_tag="ACHKV2_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO6941182.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(3812..5959)
                     /gene="cadA"
                     /locus_tag="ACHKV2_003352"
     CDS             complement(3812..5959)
                     /gene="cadA"
                     /locus_tag="ACHKV2_003352"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO6941183.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6039..7373)
                     /gene="cadB"
                     /locus_tag="ACHKV2_003353"
     CDS             complement(6039..7373)
                     /gene="cadB"
                     /locus_tag="ACHKV2_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO6941184.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7739..9277)
                     /gene="cadC"
                     /locus_tag="ACHKV2_003354"
     CDS             complement(7739..9277)
                     /gene="cadC"
                     /locus_tag="ACHKV2_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO6941185.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9537..9696)
                     /locus_tag="ACHKV2_003355"
                     /pseudo
     CDS             complement(9537..9696)
                     /locus_tag="ACHKV2_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9894..9969)
                     /locus_tag="ACHKV2_003356"
     tRNA            complement(9894..9969)
                     /locus_tag="ACHKV2_003356"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9934..9936),aa:Phe,seq:gaa)
     gene            complement(10076..10651)
                     /gene="yjdC"
                     /locus_tag="ACHKV2_003357"
     CDS             complement(10076..10651)
                     /gene="yjdC"
                     /locus_tag="ACHKV2_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6941186.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(10688..12385)
                     /gene="dsbD"
                     /locus_tag="ACHKV2_003358"
     CDS             complement(10688..12385)
                     /gene="dsbD"
                     /locus_tag="ACHKV2_003358"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO6941187.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(12361..12699)
                     /gene="cutA"
                     /locus_tag="ACHKV2_003359"
     CDS             complement(12361..12699)
                     /gene="cutA"
                     /locus_tag="ACHKV2_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO6941188.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(12815..14116)
                     /gene="dcuA"
                     /locus_tag="ACHKV2_003360"
     CDS             complement(12815..14116)
                     /gene="dcuA"
                     /locus_tag="ACHKV2_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO6941189.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(14234..15670)
                     /gene="aspA"
                     /locus_tag="ACHKV2_003361"
     CDS             complement(14234..15670)
                     /gene="aspA"
                     /locus_tag="ACHKV2_003361"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO6941190.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            16007..16483
                     /gene="fxsA"
                     /locus_tag="ACHKV2_003362"
     CDS             16007..16483
                     /gene="fxsA"
                     /locus_tag="ACHKV2_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO6941191.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(16499..17755)
                     /gene="yjeH"
                     /locus_tag="ACHKV2_003363"
     CDS             complement(16499..17755)
                     /gene="yjeH"
                     /locus_tag="ACHKV2_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO6941192.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            18031..18324
                     /gene="groS"
                     /locus_tag="ACHKV2_003364"
     CDS             18031..18324
                     /gene="groS"
                     /locus_tag="ACHKV2_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO6941193.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            18368..20014
                     /gene="groL"
                     /locus_tag="ACHKV2_003365"
     CDS             18368..20014
                     /gene="groL"
                     /locus_tag="ACHKV2_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO6941194.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            20152..20505
                     /gene="yjeI"
                     /locus_tag="ACHKV2_003366"
     CDS             20152..20505
                     /gene="yjeI"
                     /locus_tag="ACHKV2_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO6941195.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(20698..21567)
                     /gene="yjeJ"
                     /locus_tag="ACHKV2_003367"
     CDS             complement(20698..21567)
                     /gene="yjeJ"
                     /locus_tag="ACHKV2_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO6941196.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         5758 a         5176 c         5279 g         5356 t
ORIGIN      
        1 aggttaagat cattgatgta tgatatgaat ttctcttcta tcgtggctga tgaatatggg
       61 ataccacgac aattgaatga aaattccttc gcaataacga catcgttagc cgcaagtgaa
      121 atcgaagatt taatcaggct taaatgctta gacttaccgg atattgactt tgacctcaac
      181 attatgacag ttgatgacta tttccgtcag ttttacaagt agccagtaag gataagaaga
      241 acataaaaaa tggcactatt ctctaaaata ttaatttttt atgtgattgg tgtgaacata
      301 tcctttgtca ttatctggtt tatctcacat gagaaaacac atattcgttt acttagtgca
      361 ttcctggtcg gaataacctg gccaatgagt ctgcctgtgg cattactttt ttctctcttt
      421 taggagctgt agttgagtgg acccgctggt tatcgtgaaa cgaagcgtct ctcctcaaaa
      481 ctggatttgc ggaacgatga acgaagcagt cgcctgactg cttcgttcat taaagtgaaa
      541 tttatttctg tgggcgcatc gccgggaaga gaataacgtc gcgaatagta tggctgttag
      601 taaacagcat aatcattcgg tcgataccaa tacccagacc agcggttggc ggcagaccat
      661 attccagcgc agtcacgtaa tcttcgtcat agaacatggc ttcgtcgtca cctgcggctt
      721 tagcgttaac ctgttcctgg aaacgttcag cctgatcttc tgcgtcgttt aattcgctaa
      781 aaccattacc gatttcacga ccaccgatga agaattcaaa acggtcggtg atttccgggt
      841 taacatcatt acggcgtgcc agcggtgaca cttctgccgg atattccgta ataaaggttg
      901 gctgaatcag atgtgcttct gccacttcat caaagatctc cgtgacaata cgtcccagcc
      961 cccagctttt ctctaccgta ataccgatag attcagctaa tgctttagca gcatcaaaat
     1021 tatccaggtc ggccatatcg gtttctggac gatatttttt gattgcttcg cgcatggtga
     1081 gtttttcaaa cggtttgccg aaatcaaaca catgctcgcc ataagtgact ttagtggtac
     1141 ccagaacctc ttgtgccagg gtgcggaaca gggactctgt cagttcaatc aaatcgtggt
     1201 aatccgcata cgccatgtag agttccatca ttgtgaactc aggattatgg cgaacagaaa
     1261 taccttcatt acggaagtta cggttgattt cgaatacccg ttcaaaaccg cctacaacca
     1321 gacgtttcag atacagctcc ggcgcgatac gcagatacat atctaaatcc agagcattat
     1381 gatgggtaat aaacgggcga gcagatgccc cacctggaat tacctgcatc atcggggttt
     1441 ctacttccat aaagccgcgc gcgaccatga actggcggat agcggccaga atttttgaac
     1501 ggacaacaaa cgtttgacgg gatttatcgt tagcgatgag gtccagataa cgttgacgat
     1561 aacggacttc ctgatcctgc agaccatgga atttatctgg taaaggacgt agtgctttag
     1621 tcagcaggcg caactcagta cagtgaatgg aaagctcacc cgtttgcgtc ttaaacagcg
     1681 taccgcgggc accgataatg tcacccagat cccatttttt aaactgatcg ttataaacac
     1741 cttctggcag gctatctcgt gcgacgtaca gttgaatacg gccaccgaca tcctgcaacg
     1801 ttacaaagga ggctttcccc atgatacgac gggtcatcat tcggccagca accgagactt
     1861 caatgtttaa ggattccagt tcctggttat ccttcgcatc aaactcttcg tgcaactggt
     1921 cagaggtatg gtcgcggcga aaatcattgg gaaacgccac accttgctga cgtagtgccg
     1981 ccagtttttc gcggcgattt ctcagttcat cgttaaaatc aatagcctca ttggctcccc
     2041 gtgtttcttg ttcagacatt ttggttcctc taaatccagc tttcaattta gcgtagataa
     2101 agagacagat cggtacactg tattatctgc ctcgactatt aataactcag tttatcttat
     2161 cgaaactaac gcttacctca agttgatatc actaataaat aaccatcaaa atggtttaaa
     2221 tgacttatta atctcgattt actagtgtga taaactgaaa gttaatttat tcataaccaa
     2281 ttgtttttac gacagttaat cgttgctctc ctgtaccata ttcgtcggcg tgctaaacag
     2341 gaaacgggta gcaaaggcca ataccacgat gatcgcgaca caagccaacg tccactctcc
     2401 catctgagaa aagaatcgct ggtaagctgc aattgccata ccgctaattt gcgactctgt
     2461 cgtctgctgt gccactacgc ctgccagcca gttggcgacc gcgcctgttg ccagcatata
     2521 aatcccggtt aatacgccag acattttcag acgcgtgatt tgcgcaatcg ccaccgggtc
     2581 aataaagagt tcggcaaagc ccattagcgc cagcccggat accatcacgc ccattgacgc
     2641 ttgaccgtca gctgctgcat gtcgggcatc aaatgccagc aacataaagc cacaagccat
     2701 cagcagtaag ccaaaggcaa acttcagcca gacgcgcaat gttgagttgc cgtggctttc
     2761 tggcgacgcc agccaggcca gaacaacccc agcgagcatc accgcaattg cattcaccga
     2821 ctggaatagt gcagtaggta cttcaatatt gaatgcctga cgattcacaa agcgatcgat
     2881 aaacaagctg atggtactgc cgccctgttg tgccagtacc cagaacaatg tcccgacaaa
     2941 catcaacaat acaatttgcc aaagagcacg gcggtgttcg gggaatttga tcatcatgcg
     3001 ggcaatgatt tgtgcggcaa tgaggcaaac gatcgccagc aaatatccgg accagtcgtt
     3061 ctccagcagc agggtaaaaa atactggggc taaacagagc atcaccacta accagctcca
     3121 taccggtaag gcaaatttga cgctagtgag cgctttttta tccatactac gcgtggactg
     3181 gaaatgacga tgcccgctta agaaaatcaa caaaccgata aacatgccgc cacccgcaag
     3241 ggcaaagcca acatgccatc cataccactg agcagccagg ccgcaggcga ttggggcggc
     3301 gatagaaccg atattgcccg cagcatacag cagcgaaaaa ccgccatcac gtcgatgatc
     3361 gttctcgtcg tagagctcgc caagcaaaca gctgatgttt gatttgaata aaccgtagcc
     3421 acaaataatg attgccagcg ccagatacag gctaaaggtt gaatttgtat caatacccag
     3481 caccacatgg ccaagggtca ttaacagcgc gccggcaatc actgcagtgc ggttgccgag
     3541 caggcggtcg gcaagccagc cgccgagaat aggggtaacg taaaccagag aagcatatgc
     3601 gctgaacagg ctgatggcat ggttatcatc aaaaccaagc tgatgggtga gatagagaat
     3661 gagtaaggca cgcatgccgt aaaaactgaa gtactcccag atttggatcg ccacgatata
     3721 gtatatcgcg cgcggctgtg agggtgtttt catgtgttct ccttatgagc aaaaaaggga
     3781 agtggcaagc cacttccctt gtacgagcta attatttttt gctttcttct ttcaatacct
     3841 taacggtata gcggccatca gcctgacggt atgcaccgtg aatatcggtt tcaaagcccg
     3901 gatagtgagc gccgatttca cacagcatct gcaggaactc cagaaccgga cggctttctt
     3961 cggtgatcat ttcacccggc attaccagag gaactcccgg cggatacgga aggatcatat
     4021 tggcgttaat acgaccgacc atttcgtcga ggtaaacttc ttcggtcata ccgtgcagct
     4081 ctttctggaa tgcagcatac ggagtcatta ccatcgtcgg cagcacttca aatgcgcgat
     4141 acatcagatc cggcagattg tggtgaacaa tcagtttgtg gatattctga gccagttcct
     4201 gaatacgcat gttttcatag aattcaggat cttcacgata cagagacggc agcatgtttt
     4261 tcacacgcag gttcaggtcg aacgcacgtt tgaagtcagt cagagcacgc agcaggctca
     4321 gtgctttggt cttatcgata ccgatgctga acaggaacag caggttatac ggaccggttt
     4381 tctcaacaac gatgccatgt tcgtcgaggt atttcgccac gatgctggcc ggaataccaa
     4441 agtcgctcat ggtgccgtct ttttccatcc ccggagtcag cagggtgact ttgatcgggt
     4501 caagatacat gtgctcgtta tcgatgtttt tgaagccgtg ccaggtgctg tcagaacgca
     4561 gcggccagca ttcagtcgta tcgatatgat ccggctgcca tacatcaaag aaccagccat
     4621 cagattccgt tctcagacgt ttgatctctt tacggaattt gatcgcacgt tcaatggaac
     4681 cgttgatcag acgcttacct gcattgcctt tcatcatcgc cgcagcggtt tcagtggacg
     4741 ccacgatacc gtagtgcgga gaagtggtgg tgtgcatcat gtaggcttcg ttaaaggttt
     4801 cttcgtttac gtcaccttta acgtggatca tggaagcctg agagaacgcc gccagcagtt
     4861 tgtgagtgga ctgggtttcg taaatcactt tcccttctac acggccaccg ctcataccgc
     4921 atttaccttc gtaaatcggt gagaagttgg tgtaaggcac ccacgcggag tcaaagtgga
     4981 tggatttcac atccagtgtt ttcttgatga agtcggtgtt gtacagcaga ccatcatagg
     5041 tagagttggt aattacagca tgtaccggcc aggttgcgtt tggtgtttct ttcacgcgct
     5101 tagcaatggt agcgtgctgg aattcactct gtgggatacc accaagaata ccgtaagcgt
     5161 tacgggtcgg gcggaaatag attggcgtaa catcgctcat catcatcagg tgggtcagcg
     5221 atttgtggca gttacggtca atcagaatgg tgctgcctgc cggagcagag tacataccaa
     5281 caattttgtt cgcagtggaa gtaccgttgg tcaccatgta gctgcggtct gcgttaaaga
     5341 cgcgagcgat atactgttct gcttctttgt gtggaccact gtgatccagc agagaaccca
     5401 gttcagatac tgaaatggaa atatcagatt tcatggtatt cggaccaaag aaatcataga
     5461 acaggctacc taccgggctt ttctggaatg cagtaccacc catgtgacca ggagtacaga
     5521 aagtatattt accttcacga acatatttaa acagtgcttt agtcagcgga ggcagaatag
     5581 tgttgatata ttcgtcagtg gtctgcttga tcttgttagc aatatcttca gcagcaccca
     5641 gcgcatattc aaagaagcta atctgtaaac gcaggtcatt caggcttaca tcgagagtgg
     5701 aatacgtatt agcgaacgcg tacaacggca ggttctcgtt cattttgcta atttcttcgc
     5761 acagctcgag attatattta tcccagtcaa aaataacgcc gcacagacgc gcattgtttt
     5821 cgatcagttt taataagtcg tcacggtcgt tcgggtaaac aatctggaag ttcagacgtt
     5881 caagcgcgcg atgaagttca cggatgggtt cttctttaaa ataaaccccc atgtgattca
     5941 atattgcaat aacgttcata gtcatatctc caggtaaaaa aggcccctcc caacacatgg
     6001 gacaaaatga aaggaggagc ctcggaaaat acttttaatt aatgtgcgtt agacgcggtg
     6061 tggttatcca ttgagtggct ctggcgctcg tgcattttgc gagcgtagaa catcaggata
     6121 atcaggctga cgatgaaggt acctgccagc tcgaaggagc ttgcgcccat cagcgcgatg
     6181 aagcagaaca cgcaacccag tacagagcag atcaggctga caaagttgcg gatgttaacg
     6241 ccttcaaaac gaatcaggtc aacgcaagag tagaagtacg gcagcatagt cagcagtact
     6301 gcgataccgg tcagttcacc gaacaggtca gatgctttac caccggcaga gttcatcaga
     6361 gtgatgagga tcatcagggc agtcattttc actgcagcca gcagcagacc ttttttcgga
     6421 ataccgttgc tgtcgacttc accataaact ttcgggaagt taccgtcgtt agcggcacgt
     6481 acacctgcct ggcctaccaa catcatccag gagcccagag aagtcaggca cgcaaaggcg
     6541 gtgaatgcag aaaccagcgg cgcagcccag ttaccgagga tagttgaagc actgattgca
     6601 aacggagcac cggaagccgc cattacagaa gacggataca taccggaaag cacctgagtc
     6661 gcagcgatgt aaacaatacc tgctaaacca gtacccagca tggttgccag cggaacggta
     6721 cgtttcgggt ttttaaccat accagtactt acagctgcgg attcaacacc cacgaaggcc
     6781 cacaggcaga gcagaatact tttaatgatc gcatgaccat cagtggtatc cgcagtattc
     6841 cagttagctg cataagttgc cgcatcaaac caatgccagc caacaatagc agtcatcacc
     6901 acaggaataa gaaccagcac cagaccaata gtggttaaac ggcttaccca ggtaccgccg
     6961 agcatattta caaaggtaaa tacccagacg atagcaatac aggcgatacc cgccggaaca
     7021 ggatcattta atactgggaa gaaggtggaa agataagata cagcggtaat accaatcgcc
     7081 aggttaccaa tccagttagc atggtaataa agaacacctg tctgaaaacc aaatgcagga
     7141 gaaatttctc cggcataagc aattgggcca ccttgttgcg ggttttttgt tgccagtcgg
     7201 gcatatacat acgccagcga cattgcacca ataatagaga taatccaacc ccagatagca
     7261 ataccaccga tacttgctag gttcgcaggt aataatgcaa taccgctccc catcatatta
     7321 ccggcaacaa caccggtaca ggcaaatagc ccgatcttct tggcagaact catgctcttc
     7381 tcctaatttc atttttgaat ttggagtccg ggtcatgatg tataactatt tcctgaccag
     7441 accaaactgg cgctaagatt actcacgaaa aaaggattaa tcctaaagat taggtgaaat
     7501 aacacaaaag tttctgtaag tgagaacttg aggtttttta ttaacacatc aggatcgcaa
     7561 gttgatatca tgaaaagata aacattcaat gtttacaata gattgcgtga cgtcttctgg
     7621 ttaaatttat gtaataaaaa ttatgcggca aataaattgc cgcaacatat tataccaaca
     7681 ggaacataca aaaactcaac aacaaatatt cccgagcatg aatcaacccg ggggttactt
     7741 attctgaagc gagaaatttg tcgagataag gtacaacata aggaacagaa gtctggaata
     7801 taccattttc aatccagtaa agggtgtttg cccctgggcg taaattaaag gcggtgagat
     7861 atgcatcagc cgcttcccgg ttcatcccct tcatttcata aaccttgcca agcaatacat
     7921 aatttagcca ggacatttca agatcaatgc cagtatttat cgcctggtaa gactcatctg
     7981 ttttaccttt taccagagca ctgaccgctt ttatttgata tataatggac aggttattca
     8041 attccggtag tgtaacaatg ttatctattt ctgtgttcag tgctgctaat tgtttttcat
     8101 ctaaaggatg ttgagaatgg cgcacgatat caactaatgc tttttctgct ctcgcgtagg
     8161 taaattctgg ggatgattga acaatctcac ctaataattc actggcacgg ttcaatgatt
     8221 tatcatcgcc atgcagtaaa taatcatgtg cctgataaaa attagttaat aacgcaccac
     8281 gatgaggcaa aatttgctgg agcgtctcct gcattcgttg tggccacggt tggtttaacg
     8341 cttttgataa actctccagt aaatcatttt gaatcgccag ctgattaccg ttagtgatga
     8401 cataacgttt atccagcatg gttgaaccat ctgcattgtc taccaatttt atcgacataa
     8461 agcattgttg agcacggtat tggcgctgat taacaaacgc aatagataat gttttaccgg
     8521 aactgctcgg ttcatcaatg ttgtagttga ttttgtcatg caccataaag gtggagaagg
     8581 tgttaagtga tgtcgccacc aaatcaccca cgcctatcgc gtaagagagc tgatacgggg
     8641 aactccagct gttacaactt ttatttacca tattaatgtc aatatcgcgt ggattgagca
     8701 aaatacgcga tttgctcatc ggaagacgtg tatcaagact tgaaaacgct accagtgcta
     8761 cacagatacc taaggacaac aggaaaaaaa accataccca aaaggtagtg aatcgtttgc
     8821 ttttaactgg ggattgttca ggtggcgttg cggtgttttg aatgttaaga ctgtgggagg
     8881 gagaatctgt ggcaggaacc gcctctggta tagggggagg cgaagatagc attatttcct
     8941 ctccctcttc ttcgctgtac cagataaccg gcaccattaa tttatagccg cgctttggta
     9001 cagtagcgat atagacagga ctatcttcat cattatcttt taatgactta cgtagttctg
     9061 agatactctg cgtcacaacg tgattggtga caatacttct cttccagaca ttatcgataa
     9121 gttcatccct gctaagtact tcgccactgt gttgagcaaa gaaaaccaga aggtcgatta
     9181 atctcggctc aagggtaagt tgacgcccat tgcggctaat ttggtttatg gacggagtaa
     9241 caagccattc gccaacgcga actacaggtt gttgcataat agaaactcat tcgaaaaggg
     9301 aatgatgcaa tgataattgc cacaacctat ttttaccatc tatagatggg tttatttaca
     9361 tattattggt gaatgcaaga cgttattttt accagccata aacttcttga ttacatggta
     9421 ttacgaaagg attttactga gaaccagaag taatattcct taccatcaaa attcatcatc
     9481 tttgccaaag aaaaatgttc agaaaataat ccatggaaaa ttgtccggag cgcttactat
     9541 tttaatggat tgttagtctt tgcatgagca agcggactga tacatttctc tttgttctca
     9601 ttcagaaaat ctcatcagtc ggcgttcaga ccgatgttgc gctttatgga tgcgttacgc
     9661 tcctgatgac gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgttag
     9721 ttagtgctat agctcaggaa caaattttcc cgaattggga tatgcccgca aattgctggt
     9781 gatgtgggga gaatcgggtt gagttcggta gaattgattt ggaggcagaa cacttaaatc
     9841 gtggcgtcct gaaacgaaaa acggacctcc gtggaggtcc gtttatatga atttggtgcc
     9901 cggactcgga attgaaccaa ggacacgggg attttcaatc ccctgctcta ccgactgagc
     9961 tatccgggca acggggcgca ttaaaccgta atctgcacat ctcgtcaacc taatttcagt
    10021 aaaagcgatt caactgctta agattgcggc aaatcgcttc ttttctgtgt ttctgtcagg
    10081 tcagtgcacc accctggcgg cagcgggcga agcgcaggat atcttccgcc agccgatgtg
    10141 cggtgtcgac atccgcctgg ctacgattca ccagcattcg gcttaaacag ccttccagca
    10201 ccagttccat ctgctttgct accatcgccg gatcgtcaac ttccagcgtg gttaacagtt
    10261 cgtgggtgaa atcgtaggcc gcgctttttt gctgatcggc cagttgatga atagggtggc
    10321 caggatcggg ataaaacgta caggcagcga taaacagaca gcccggatag cggttgtttt
    10381 taacgcactc cgataacgcc tgataacgtg ccagcagctt ttgttcggcg gtttgcgttt
    10441 cgtccagcat cagctgacga cgccagacat ctatctgttg gctaagataa cgcagcgcat
    10501 cgtagaggat tgcctctttg tctggccaga agcggcgtag ctcgtccagt ggataatcca
    10561 cacgttcagc aaccatctcc agcgtggtgt tggcaatccc ttgtaattct aataatttca
    10621 gggcttctcc cagtacatct tcacgttgca cgctattttc ctccgtcttt cccactgcaa
    10681 gtgtcgttca cggttggcga tcgcgcaaat gtgcgctgaa ggtttcagca tccataaagc
    10741 ccgtgacgcg tgcttgtgga tgctcctggc cttgtccgtc aaaaaagaga attgtcggta
    10801 ggccaaggac attaagatgc tttaacagcg ccacatcttg tgcgtcgttg gcagtgacgt
    10861 tggcctgaag taataccgtg tctgctaacg ctttttgcac ctgcgggtcg ctgaaggtgt
    10921 atttctcaaa ctctttacag gcgacgcacc agtcggcata aagatctaac atcaccggtt
    10981 tgcctttggc ttcaacgagc gcctgattta actcatctac cgttttgatt tgtgtaaagt
    11041 tgagatgcgt ctgagtttgc gcggtatgcg tcgcaccaaa tgcccaatcc tgaagtgggc
    11101 gcacgctaac caatgccgct gcaagcagga ttatttgcac cacgcgcatc cagccgcgtt
    11161 tggcctgtag gctggtgata aaggcccagc caaagaatgc gacacccagc gccgaccaca
    11221 agcgtaatcc ccatacatca ccaatcactc gctccagcag gaagaccggc agtgcgagga
    11281 tcacaaaacc aaacgcggtt ttgacttgtt ccatccacgg gccgcttttc ggcagcaagc
    11341 ggttaccaaa gacggtaatt agcatcagcg gcaggcccat gcccaacgca tagagataaa
    11401 gcgtgccgcc gcccagccac atgttcccgc tttgggcgat atacagcaga atcgcgctaa
    11461 gcggtgcggt ggtgcatggt gaacagatca gtccggcaat cgtccccata ataaacactc
    11521 cgccgggtga gccgccctgt tggcgattgc tcatcagcgt gaggcgcgtt tgcagcgagg
    11581 aggggagttg tagagtaagc aagccaaaca ttgacatcgc cagcaaggta aagacgatgg
    11641 tgaggccaat gagcacgtat gggtgctgta gcgccgcctg gaactgtaac cctgcggcgg
    11701 caactaccag acccagcgcc gtgtaagtca gtgccatccc ctgcacataa ataaaggtca
    11761 gcaacaatgc tctggcagtg gagagccgct gtttaccacc cagcacgatg ccagaaatca
    11821 gtgggtacat tggcagcacg catggcgtaa aggcgatacc aataccgatc aacaacgccc
    11881 agagcgcgga aaagggcaat tgcgcggtgg gctgctcttg ctgcggaaca gacacaggct
    11941 gtgacgctgc gttgttggcg accacttcgc ttaacggaac ggttttggtt tctggcggat
    12001 aacagaaacc ggcatcagca cagccctggt aggtgacagt taacgtcgct cccgcactcg
    12061 cctggttgat ggttacggga agcgtcagcc gatcgcggta aatctcgctt ttgccgtaaa
    12121 actcatcttc atgccagacg ccttgcggca gctgcacgtc ggcaattttc gcgtgttccg
    12181 gcgtaatgcg gatctgttta cggtagaggt agtaaccgtc tttgatctgc caggtcagat
    12241 taaggtcatg ttggttttgc tgaaaatcaa aagcaaaggc ttgatccacg gggacaaatt
    12301 gtgaacgtcc cggcgcgtcg aataatccgg caaaaacgga agtgctgcaa agtagcagga
    12361 tcagcgtaaa gatgcgttga gccatgagag gtaatctgtg tctccgtgtg taacaggtaa
    12421 aaccagaagt tccggggttt gatatggatg atgagacttc aggcattcca gcagtgcctg
    12481 ctggtgagat acggtagttt ttaaaatcat ctgcacttcg tattcttgct ccagcttacc
    12541 ttcccagtaa tagagagagg tagcgccggg gatcaaggtc gcgcaggccg ccagtttttc
    12601 cgccagcact ttggcggcta aatcctgggc tgtcgcttca tccggtgcgg tacatagcac
    12661 cacgacagac gcggtattcg aacttttttc atcaagcata aacacctcgc aagaacagat
    12721 ggaaccgcaa gagagaggtc actatacaac ggacggggaa aggatgttag tcggcgagaa
    12781 agaaagcggg gcgtgaacgc cccgcgattg gtcattacag catgaagcta cccagcacga
    12841 agccgaagca aacggccagg gcaacaccca gagtacccgg gatgaagaac ggatggttga
    12901 agacgaattt accgatacgg gtagtacccg tgtcatccat ctgtaccgca gcaaccagcg
    12961 tcgggtaggt cggcagaatg aacagaccag acaccgcagc gaaagaagca acagcggtca
    13021 gcggagaaac gttcagtgcc agagccatcg gcatcagtgc ttttgcggtt gcagcctgag
    13081 agtacagcag agcagaagca aagaagaaga tgacggccag cagccacgga tgaccctgaa
    13141 tcacttcacc agcggtatct ttgatccagt cgatgttgtt ggaaacgaaa gtatcgccca
    13201 gccacgcaac acccaggata caaatacagg cgctcatacc tgctttgaag gtgctggagt
    13261 tgaggatgtt gtcggtatcg actttacaga taacggtggt cagagttgca acactgagca
    13321 tgatgatcag gattgcgttg gtggtgttca tcagcggttt ttcaaccaga cccatgcttg
    13381 ggctgttgat gattgcatag ataaccacgc caactacgcc cagcaggaac agccagacgg
    13441 acgtttttgc accggatttg atttcaatct gcttttcacc gcgcagttca accaggccct
    13501 cttccagacg cttgcgataa atcggatcgt cagagagttt ggagttgaac agcatagtga
    13561 ccaggaagga catcaccaga accgccagca gggtggacgg gatgaccacg gagagcagat
    13621 ggaggtagct gatgccatga ccttccatca cggaagacat gtaaaccact gccgctgaga
    13681 ttggcgatgc ggtgatcgca atctgcgcgg ataccactgc agtagacagc ggacggcaag
    13741 gtttaacgcc ttgttccttc gcaacttcag cgataactgg cagtgtcgcc agagagatgt
    13801 tgccagtacc agcaaagata gtcaggaaat aggtcacgat cggtgcgagg atcgtgatgt
    13861 atttcgggtt acggcgcagc agcttttctg tctgatgaac cagatagtcc agaccgccag
    13921 caacctgcat ggcagaaata gcggcgataa ccgccatgat aatggaaatg acatcgaacg
    13981 ggatgttacc gggtttaacg ccaatagcgg caagaaccag cacccccaat ccgcctgcaa
    14041 aaccaatacc tattcccccc aatctggcgc ccaagaagat cgccagcaaa actatgatga
    14101 gttctacaac tagcatatta gccttccttg ttttttaaca agttgatatt agattgttat
    14161 ttttaagtta ctgctcacaa gaaaaaaggc acgtcatctg acgtgccttt tttatttgta
    14221 ctaccctgta cgattactgt tcgctttcat cagtatagcg ttttgctttg taagccgggt
    14281 gcatcagatt ctgtacggag aaaatatcgt caagttccgc ttcagtcaac agaccgcgtt
    14341 ccagaacgac ttcacgtaca ctcttaccgg tttcggcaca gattttaccc acgatgtcac
    14401 cgttgtggtg accgatgaac gggttcaggt aagtaacgat accgatagag ttgtaaacgt
    14461 aaccttcgca cacttctttg ttagcagtga tgccgttaat gcatttttcc agcaggttgt
    14521 agcaagcgtt ggtcagaatg tgaacggatt cgaacatagc ctggccaatg accggctcca
    14581 taacgttcaa ctgcagctga cctgcttctg ctgccatggt aacagtggtg tcgttaccga
    14641 tgactttgaa gcatacctgg ttaaccactt ccggaacaac cgggtttact ttagctggca
    14701 tgatggaaga gcccgcctgc agttccggca ggttgatctc gttcaggccg gcacgtggac
    14761 cagaagagag caagcgcagg tcgttacaga ttttggacat cttcacagcc aggcgtttca
    14821 gcgctccgtg aaccataaca taagcgccgc agtcagaggt cgcttcgatc aggtcttcag
    14881 ccggtacgca tgggaagcca gtaacttcag ccagtttttt cactgccagc ggagagtact
    14941 ctttcggcgt gttcagacca gtaccgattg ctgttgcgcc aaggttaact tccagcagca
    15001 gttcagcggt acgttggatg tttttcactt cttctttcag caggatgctg aaagcgcgga
    15061 attcctgacc gagggtcatc ggtactgcgt cctgcagctg ggtacgaccc attttcagga
    15121 tgtcctggaa ttcgaccgct ttacgttcaa agccttcacg caattggtta atcgcatcta
    15181 ccagcttaat cagggaagag taaactgcga tacggaaacc ggtcgggtag gcgtcgttag
    15241 tggactgaca tttgttaaca tggtcgttcg ggttcaggta ctgatattca cctttctggt
    15301 gacccatcag ttccagaccg atattggcca gcacttcgtt ggtgttcatg tttacggaag
    15361 tacctgcgcc gccctggtag acgtctaccg ggaactgatc catgcatttt ccgttgttca
    15421 ggacttcatc acatgcggca atgatggcat tcgctacact tttaggaatg gtttgcagct
    15481 ctttgtttgc catagctgcg gcttttttaa ccattaccat accgcgaaca aattcaggaa
    15541 tatcactgat tttgttgttg ctgatataga agttttcaat cgctctcaga gtgtgaacac
    15601 catagtaggc atcagctgga acttccctgg tacccaacag atcttcttcg atacgaatgt
    15661 tgtttgacat gtgaaccttc tttttcaagc tgccaatgat ttgctttaaa cacacagaat
    15721 atatgtggtt tcgaatgttt ttcgaccgac gattatcccc tgcatcgacc gaatatccga
    15781 gatcatatgc tgcttgagga tttctaccgt aatctggatc actttaagtg tcggttttta
    15841 ccccttaatt attaatttgt gaaatagatc accgctttgg gattactacc aaaaatagtt
    15901 gcgcaaatat cttgaaattt tgctaatgac cacaatataa gctgaacgcg attcgcaacc
    15961 cattcaggta gccgggttta accggctgct attacaggag aaacctttgc gctggttacc
    16021 ttttattgcc attttccttt atgtctatat tgagatttca atctttattc aggttgccca
    16081 tgtattgggg gtattgctga ctctcgtgct ggttatattt acgtcagtta tcggtatgtc
    16141 actggtacgt aaccagggct ttaagaattt cgtgctgatg cagcaaaaaa tggcggcggg
    16201 tgaaaaccca gcggcggaga tgattaaaag tgtttcgctg atcattgccg gtttgctgct
    16261 tttattaccg ggctttttca ccgacttcct cggtcttctg cttttattgc cgccggtgca
    16321 aaagcatctg acagtgaagt tgatgccgca tttgcgcttt tctcgcatgc caggcggcgg
    16381 ttttagcgcc gggaccggtg gcggtaatac ttttgatggt gagtaccagc gaaaggatga
    16441 tgaccgcgac cgccttgatc ataaagacga tcgccaggat taatgtcgaa acgccggatt
    16501 atgtggttat gccattttcc ggcgtttttc gttttggcag aaacagccat aaccccgcca
    16561 gcatgatcag cgcatagaga cttttccagc cgaccattgc cagtaacaga acgcataaca
    16621 gcccgccaac caccgccagt agtcgataac gaccttgcaa taatttacag cctgccagca
    16681 tgcataacag ataaatcata ataaagatgc cattggcata aataataaga gcgtccagat
    16741 tgatctctaa agcatgaatc accaacgtgc tcaccacgca gcagccgagc accgcattga
    16801 gggcattatt cgggatatgg cgagaagaga ggcgtgccag gtagtggtca ggattatgtt
    16861 gcgcctgcga ccagaccagg cgggcgaagc tctgtatata aatgttgaga ctggcaaagc
    16921 aggccagata gccaattacg caggcaatcc ataacgctcc tacaccgaac agttgtacta
    16981 caatttttgg aagcgatgct gccgcggcca tttgttcacc ataggcgtcg aagtgtaaga
    17041 cgactaccgt acagccccag tagactaatc ctgccagcag cagaccaatc atcaaagcac
    17101 gaggaaaatc acgttctgga tttttaaatt ccgaggcaag atgggcaaat gcttccagac
    17161 cgacaaaaca ccagaacatc actgataacg cagcaaataa cccggtaagt tcgatatttc
    17221 ctggcgcggg gaaggggata ctcgcaggtt tgatatcgcc cgcccaccag atagcgacaa
    17281 tcagtgcgac gataagtccg gcaataactg tttgtagatt tgcactggaa ctggcaccgc
    17341 gagtaccgat ataccacacc aacgccagcg taccgagttc tgccaacaac agttgccagc
    17401 tatgccagcc aaacattgcc tggccgaatc cggcagcaat ttgtagcgcg gcaggcaaac
    17461 ccacgggaat gaccgataaa aacagccagc cggtgactcg ctcaagccgc gaaccaaacg
    17521 ccataccgac gaagtgcgcg acgccgcctg cgctgggata gtggcgaccc agaatcgcaa
    17581 acacaatcgc aatcgggaac actaagataa tcaaaatggg ccacgcccac aggctgttat
    17641 tgcccgctac cagtgcagct aacgcaggaa cggcaaacac gccagtgcct aataatgacg
    17701 tcgatagcag gccaatgccc tgggccagcc ccagttcttg tttgagtcca ctcatgggtt
    17761 gatgtccgat tgcgcccaaa ttttgggcaa ctgcgtagat tttcgatggt agcacaatca
    17821 gattcgctta tgacggcgat gaagaaattg cgatgaaatg tgaggtgaat cagggttttc
    17881 acccgatttt gtgctgatca gaattttttt tctttttccc ccttgaaggg gcgaagcctc
    17941 atccccattt ctctggtcac cagccgggaa accacgtaag ctccggcgtc acccataaca
    18001 gatacggact ttctcaaagg agagttatca atgaatattc gtccattgca tgatcgcgtg
    18061 atcgtcaagc gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct gaccggctct
    18121 gcagcggcta aatccacccg cggcgaagtg ctggctgtcg gcaatggccg tatccttgaa
    18181 aatggcgaag tgaagccgct ggatgtgaaa gttggcgaca tcgttatttt caacgatggc
    18241 tacggtgtga aatccgagaa gatcgacaat gaagaagtgt tgatcatgtc cgaaagcgac
    18301 attctggcaa ttgttgaagc gtaatcctcg cacgacactg aacatacgaa tttaaggaat
    18361 aaagataatg gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga aaatgctgcg
    18421 cggcgtaaac gtactggcag atgcagtgaa agttaccctc ggtccgaaag gccgtaacgt
    18481 agttctggat aaatctttcg gtgcaccgac catcaccaaa gatggtgttt ccgttgctcg
    18541 tgaaatcgaa ctggaagaca agttcgaaaa catgggtgcg cagatggtga aagaagttgc
    18601 ctctaaagcg aacgacgctg caggcgacgg taccaccact gcaaccgtac tggctcaggc
    18661 tatcatcact gaaggtctga aagctgttgc tgcgggcatg aacccgatgg acctgaaacg
    18721 tggtatcgac aaagctgtta ccgctgcagt tgaagaactg aaagcgctgt ccgtaccgtg
    18781 ctctgactct aaagcgattg ctcaggttgg tactatctcc gctaactccg acgaaaccgt
    18841 aggtaaactg atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta tcaccgttga
    18901 agacggtacc ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt tcgaccgtgg
    18961 ctacctgtct ccttacttca tcaacaagcc ggaaactggc gcagtagaac tggaaagccc
    19021 gttcatcctg ctggctgaca agaaaatctc caacatccgc gaaatgctgc cggttctgga
    19081 agccgttgcc aaagcaggca aaccgctgct gatcatcgct gaagatgtag aaggcgaagc
    19141 gctggcaact ctggttgtta acaccatgcg tggcatcgtg aaagttgctg cagttaaagc
    19201 tccgggcttc ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc tgactggcgg
    19261 taccgtaatc tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg aagacctggg
    19321 gcaggctaaa cgcgttgtga tcaacaaaga caccaccacc atcatcgatg gcgtgggcga
    19381 agaagctgca atccagggcc gtgttgctca gatccgtcag cagattgaag aagcaacttc
    19441 tgactacgac cgtgaaaaac tgcaggagcg cgtagcgaaa ctggcaggcg gcgttgcagt
    19501 tatcaaagta ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac gcgttgaaga
    19561 cgccctgcac gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg gtggtgttgc
    19621 gctgatccgc gtagcgtcta aactggctga cctgcgtggt cagaacgaag accagaacgt
    19681 gggtatcaaa gttgcactgc gtgcaatgga agctccgctg cgtcagatcg tcctgaactg
    19741 cggcgaagaa ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca actacggtta
    19801 caacgcagca accgaagaat acggcaacat gatcgacatg ggtatcctgg acccaaccaa
    19861 agtaacccgt tctgctctgc agtacgcggc ttctgtggct ggcctgatga tcaccaccga
    19921 atgcatggtt accgacctgc cgaaaaacga tgcagctgac ttaggcgctg ctggcggcat
    19981 gggtggcatg ggtggcatgg gcggcatgat gtaattgccc tgcacctcgc agaaataaac
    20041 aaacccccgg gcagaaatgt ctgggggttt ttcttttggt catctttcta gtataagatt
    20101 cagacacgga cgacgcgagt ggcgtccagc tcattgatta tggggaataa catgcacgta
    20161 aaatacttag cagggattgt cggtgccgcg ctactgatgg cgggttgtag ctccagcaac
    20221 gaattgagtg ctgccggtca gagtgtacgc attgtggacg agcagccagg cgcagagtgc
    20281 cagctgattg gtactgcgac aggtaagcaa agcaactggc tttccgggca acacggagaa
    20341 gagggcggtt ctatgcgcgg cgcagcaaac gatctgcgca accaggcggc tgcaatgggc
    20401 ggtaacgtga tttatggcat cagtagcccg tcgcagggaa tgttgtccag ttttgtcccg
    20461 acggatagcc agattatcgg ccaggtatat aagtgcccga actgatgtga tggtgacaaa
    20521 cgtataattg ccagatgcgc ttcgcttatc aggcctacat aaggcattgc aactgattga
    20581 atgcttgaga atttgtaggc cggataaggc gtttacgccg catccggcat gaccaacgat
    20641 cacgttatca gtcatttcag gctggatggc tccagcctcg cattagcaaa gatgagatta
    20701 tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt aagtgatgca gcgcgcgtaa
    20761 gcattgatcc tgggttagcg gttgggcgtt attcgccgct acggtgcgaa catagacctg
    20821 acatggcaca cccacgagtt ttttcagtcg tgggctgaaa tcaagcattc ggcgagtgat
    20881 cgcctctatt tctttacccg gcgttgcttc acgcgttttc accactgcgc cgctaaattg
    20941 ttctttgcca ctttcatctt taaagcggta aagcaccgcc agataatggt cgaacaagtt
    21001 atgtttccat tctggttggg aataggtgtc atattccaga ttgccatttt gctggcagtc
    21061 aacgtcatat aacggcagga aatctaacag tgaggtaata cgtaatgcca gttcacgcat
    21121 ttccgcatta ttaattgcat gaatgattgc tcgcgccagc atttcaatct gcaactcatt
    21181 gacgactaac tcacatttgc taccgtcatg tagcgttaat acgaaggtaa gattttcgcc
    21241 ttgattatcg gtaagttcca gcgtgttaac ccgacgatta atatcggcat ttttaagctc
    21301 atcaaccaga atttctggga tgttttctgc cattttctta atgactttat cgcgagcttg
    21361 ctcatactga aggcgtgcag cagcatccag tttatgcttt tgatgcagac gactttccag
    21421 cgcaatcagc aggtcgcgca gttccatgac agacataaag aacaacgatt ctttattgcg
    21481 aggttcttta attttcagcg ccagtgcaat gaaattatta ctcttgcgaa taacaccggt
    21541 attaacaccc ttgatgctta tggccatgt
//
LOCUS       DAZFTN010000081        21467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFTN010000081 DAZFTN010000000
VERSION     DAZFTN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..1178
                     /locus_tag="ACHKV2_003368"
     CDS             54..1178
                     /locus_tag="ACHKV2_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6941197.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
     gene            1190..1843
                     /locus_tag="ACHKV2_003369"
     CDS             1190..1843
                     /locus_tag="ACHKV2_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941198.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSNINNIKEIYSNDDLNCFLNNISAQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLKIILSRLKNGENTGFEHDDDFGYSFQYDAAAVGKSSFFDEAAGRK"
     gene            1873..2405
                     /locus_tag="ACHKV2_003370"
                     /pseudo
     CDS             1873..2405
                     /locus_tag="ACHKV2_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            2500..4176
                     /locus_tag="ACHKV2_003371"
     CDS             2500..4176
                     /locus_tag="ACHKV2_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HFO6941199.1"
                     /translation="MYVINQQTRDNNILTLHDVFKIYQNTGGLRLVKTEENEKYISII
                     ICGVFRIRKNKKNNKITLWGYKLRDKHTGVWTRRNSFPGKIFLFWSKKSAYDMDDWLK
                     YAISYIIKSLIEAGYSIEDKLYLFRVGDEEEHSESRYVSTLYPLSVYNSYSYGRYGII
                     HCLGKTASFNYPDNVQYISRHDLLEEIKNKIYHRALKIIGVDNELNASKALSKAIWIM
                     VDKKIFAQYSRASHCSIAYNSIRQALFEDYLDFSKRIHILDDIDFCGLWPMVGLLRQQ
                     HKKGQFMLSGKTKELYERLCFPYELSYGEFRSLRHVSLSLVYALIYNHDNASFRLTVR
                     LLRHPLIKNYPVRAIYWIIDYISIRAYPEKENDIYRICSKWLEYHRDLFKNIGFYDRN
                     TESRQTSRWEMETNQLCHAIDWLLAEERLIHKNQEWPSFWRLSDEWTRQVKNNVIPAT
                     QKWKGTGINWQKIDNCVNELITFDALCQEGQEMEHCVASYADWCASGEYIAISVLMDN
                     ERATLGLSRKEHDLTYQFDQMRGIRNQAVSRNMLIKGRHILKIINSSLKG"
     gene            4346..4921
                     /locus_tag="ACHKV2_003372"
     CDS             4346..4921
                     /locus_tag="ACHKV2_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6941200.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPF
                     HVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGH
                     QKIVPFAALIKADECIISKISSYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCEHR
                     FRQPVSEDNWQQAVSEERAIRCAKRLIQCKG"
     gene            4926..7082
                     /locus_tag="ACHKV2_003373"
     CDS             4926..7082
                     /locus_tag="ACHKV2_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO6941201.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAAKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            7557..7895
                     /locus_tag="ACHKV2_003374"
     CDS             7557..7895
                     /locus_tag="ACHKV2_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941202.1"
                     /translation="MKFIILKKDKDSTIARNDSVFPVNNFFVVLTTVSSVANSFGIVS
                     SLAKSIGISFPTGNVIETCFFGMFSLLLAAFSFVSTMGLANAVPPIKNVFPLLFFVLV
                     LITNYNIYFR"
     gene            8014..8220
                     /gene="hha"
                     /locus_tag="ACHKV2_003375"
     CDS             8014..8220
                     /gene="hha"
                     /locus_tag="ACHKV2_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6941203.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNCLPPSELESFNAAAD
                     HRLAELTMNKLYDKVPPSVWKYVY"
     gene            8501..8740
                     /locus_tag="ACHKV2_003376"
     CDS             8501..8740
                     /locus_tag="ACHKV2_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941204.1"
                     /translation="MLTQNESCLDNIACVAPPFGQFSTLIGWFLIAESILTACAGVYF
                     LRKNKKRWSRHDTVMFLFASGLAIFSTWMLEHQWV"
     gene            8792..9232
                     /locus_tag="ACHKV2_003377"
     CDS             8792..9232
                     /locus_tag="ACHKV2_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941205.1"
                     /translation="MKEYEKAALIYSLKESNTLVLRIILISILVVGVISGALYLYNLA
                     ISLFDSNPTSVQFTFCGVGCFIALLLQWISNVDANYRERIGDQPGTIKNIVQYLFAAI
                     ITISFIAMALTAILVLKDSSNIWLNITFIALLLSIFVAIISVWG"
     gene            complement(9275..13552)
                     /locus_tag="ACHKV2_003378"
     CDS             complement(9275..13552)
                     /locus_tag="ACHKV2_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO6941206.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLKAIVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQE
                     FINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSV
                     FKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTH
                     KEIEKRLNTWLNKTSYEIKHIFNQAEKTRELYKTLSPSQQIDFLQERIKEYDSREKLN
                     ERNSQQTSGREGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLS
                     DISQRDLAEQLPQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKN
                     EITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLT
                     KHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLNLSEKTLRNT
                     IRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTDGRHIIRQ
                     YHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNTK
                     LKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVL
                     KLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQES
                     ESRIEKGDALKEQATEQAQTTEQAQATEQAQTTEQAQATEQAQATEQAQATEQAQVAE
                     QAQVAEQAQVAEQAQVAEQATSSYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDE
                     NEIWEVLGHVPGDSDDIFDVASFDNENDAKEFCKIVNELGIDRTQALIQEQLTTQHDQ
                     ATAQVHNKQEIYCINFSRFHDLNEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDG
                     FDTVVLMSKTVSTDELSSYPEGALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSED
                     IKTFESLHDASEYKNKMLQEHGLNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQA
                     GDSSRESPEEILASISANEHMISGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYH
                     LAVAGKHGLERGDAVARVDVTEQQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRA
                     IENVNKLEQQKDYVYFSTHEGLNAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRV
                     ESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFE
                     REKAESDLLNHGFTREMAEDALGKQFVQAREDHQESQQQRDDSQDMH"
     gene            complement(13549..13890)
                     /gene="mobC"
                     /locus_tag="ACHKV2_003379"
     CDS             complement(13549..13890)
                     /gene="mobC"
                     /locus_tag="ACHKV2_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFO6941207.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            14260..14661
                     /locus_tag="ACHKV2_003380"
     CDS             14260..14661
                     /locus_tag="ACHKV2_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFO6941208.1"
                     /translation="MKKIESLLSPICEAINETKSKSKQAVFNMYLQNILEAKKYYTWE
                     QIADYINNNTKTELAQKTYINMTERAKKRQKCSEPKEQAKTIDVIDQNEAKDDEPRGG
                     VKGFFSNISESKKEIEHDPSATNEKFINKYL"
     gene            14679..15431
                     /locus_tag="ACHKV2_003381"
     CDS             14679..15431
                     /locus_tag="ACHKV2_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFO6941209.1"
                     /translation="MKNSINFILQGKGGVGKSFATAILAQYFIDENHMDNIVVGDTDP
                     VNTTTVKVKRLNADLIQITENSKVIQSKFDPMFESMLTNSQNTFVIDNGASTFLPLIQ
                     YFNDNCVMDMFEDVEQDVYIHTVIVGGQALADTLQGFEELKELVKGSKVKLIVWINEF
                     QGIPALENIPLIETKFIEKNKDVIAGVVVIQDRKSDAFASDIKELTEKSLTLKEAIES
                     DHFGLMAKSRLKRVFNDIYKQLNSIYDDEHGE"
     gene            15433..16140
                     /locus_tag="ACHKV2_003382"
     CDS             15433..16140
                     /locus_tag="ACHKV2_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941210.1"
                     /translation="MSTVNQPELKQPEKAVSSEDIDNFIVDVFKETGHKISKDDPVIS
                     LIFLNQKIQEKFSNELQANFTALSEGFRQVVSSVENDYIQRFKNIVETCGDLDNEIKE
                     KVEEGKNDLKETSVEVKEKLTDDIIELISGIKRNQEKNNKLYEEKLKSLSKAVKPFST
                     RTAIAICALCTLCLSAAFSGATWYVAQHEKEASLRFYARAFLDMKKITEESMRKLPKS
                     DQQNIKLKLEEIDSRKP"
     gene            16160..16300
                     /locus_tag="ACHKV2_003383"
                     /pseudo
     CDS             16160..16300
                     /locus_tag="ACHKV2_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16609..17100
                     /locus_tag="ACHKV2_003384"
     CDS             16609..17100
                     /locus_tag="ACHKV2_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO6941211.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVGHKFSIGLRNDAGELIGV
                     ATAGRPVARHLDDGLTLEVNRTCTTGERNANSALYGAVWRAAKAMGYQRCITYTQADE
                     SGASLRAAGFVRVKELPPRKSWAESSVALRSKRDPVGNGGVPRVLWEIRRMSTTGIRI
                     KGE"
     gene            17102..17389
                     /locus_tag="ACHKV2_003385"
     CDS             17102..17389
                     /locus_tag="ACHKV2_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941212.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDED"
     gene            17393..17713
                     /locus_tag="ACHKV2_003386"
     CDS             17393..17713
                     /locus_tag="ACHKV2_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941213.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEAPN
                     LPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDANLRWIHGVIVQAAWFVKASL
                     GQNA"
     gene            complement(17844..18281)
                     /locus_tag="ACHKV2_003387"
     CDS             complement(17844..18281)
                     /locus_tag="ACHKV2_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO6941214.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(18306..18488)
                     /locus_tag="ACHKV2_003388"
     CDS             complement(18306..18488)
                     /locus_tag="ACHKV2_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO6941215.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            18785..19228
                     /locus_tag="ACHKV2_003389"
     CDS             18785..19228
                     /locus_tag="ACHKV2_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941216.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAY
                     TFISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKR
                     IVINRLLSGSILTGKDEEYIYSQLEKAQQSNEREKRLQAIKEYTS"
     gene            complement(19615..19893)
                     /locus_tag="ACHKV2_003390"
     CDS             complement(19615..19893)
                     /locus_tag="ACHKV2_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO6941217.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGNIYSTLEHGKEYSEQELIKMIKEGKVISKY"
     gene            complement(20445..20696)
                     /locus_tag="ACHKV2_003391"
     CDS             complement(20445..20696)
                     /locus_tag="ACHKV2_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6941218.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
     gene            complement(20686..20946)
                     /locus_tag="ACHKV2_003392"
     CDS             complement(20686..20946)
                     /locus_tag="ACHKV2_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941219.1"
                     /translation="MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTV
                     ADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMSEGKNAK"
     gene            complement(20998..21222)
                     /locus_tag="ACHKV2_003393"
     CDS             complement(20998..21222)
                     /locus_tag="ACHKV2_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941220.1"
                     /translation="MKQSTFPAIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
BASE COUNT         6511 a         4510 c         4292 g         6154 t
ORIGIN      
        1 tgcatttcga agcaagaatc tctaccttcc cccctttttt gtctggaggg gatatgtttc
       61 agaaaatcaa aattcgcaaa atgacaatca gccgggcgct ggacaaatac ctgaagacag
      121 tatccatcca caaaaaaggc catctacagg agttttatcg cgtaaatgtg attaaacgtc
      181 accctattgc tgaacgctat atggatgaca ttaccactgt tgatattgcc aattatcggg
      241 atcaacggtt ggcgcaaatt aacccccgaa cggggcgtca aataaccgga aacaccgtcc
      301 gtcttgaact tgccctgttg tcatcattat tcaatatcgc ccgtgtggaa tggggaacat
      361 gccgcatgaa tcctgtcgaa ctggtcagaa aacccaaaat cagtagcggg cgcgatcgtc
      421 gcctgacttc aggagaagaa cgccgacttt cgcggtattt caaagaaaaa aatcaggctc
      481 tttacgtcat ttttcatctg gcgctggaaa cagcaatgcg acaaggtgaa atcttgtcat
      541 tgcgttggga acacgtcgat ctgcaacatg gggtagctca tcttccaaca accaaaaacg
      601 gtgctccacg cgatgttccg ctatccagga aagcccgtaa ttacctgcaa atgctgccga
      661 cacagcttaa cggtaatatt ttttcttata cctcttcggg attcaaaagt gcctggcgaa
      721 cagcacttca ggaactgaag atagaaaact tgcattttca tgacctccgt catgaggcta
      781 tcagccgatt tttcgaactt ggtacattga acgtcattga ggttgccgct atttcaggcc
      841 atcgctccct gaacatgttg aaacgataca cacaccttcg ggcttatcaa cttgtcagta
      901 aactcgatgc ccgacgcaag caaaccagca aaatcgctcc ctattttgtt ccctacccgg
      961 caacggtcga gaacagaaac gggcaagtcg ttgtgactct gagtgatttc gacctggaaa
     1021 caagcgcagc cacaaaagaa caggccatat ttcatgccag cgtgttgctg ctacgaacac
     1081 tggcacaagc cgcccagcga ggagaacgcg tccctactcc tggcgaactt cctacaaaca
     1141 ttgatgaacg ggtaatgatt tgtccattaa caaattagag gattcactta tgatagaaca
     1201 actacagagt gattttttat tctggatatt tatttacttc ctgatagtag gtttgcttat
     1261 cagaaacatt catcttacca gaaaaattaa atccatcgaa aaaaacaaaa tgttcccagc
     1321 ggaggaatat gctgtcctgg acattgaaca gcatatgtcc aataaatata ttgggcggct
     1381 agtaacccgt tttatgaaga gaactaatgg tgcacctttg ttaattgtgt ttccagaata
     1441 cgataaagga gacaacaatg aagacaaaaa ttctccacca ttccaacttg taaaactaac
     1501 atccaacatc aacaatatta aagaaattta ttccaatgat gacttaaact gttttctgaa
     1561 taatatttcg gcacaaaaaa atttatatac aacgcactct ggacctagcc aggcatggat
     1621 tgaacatgca tatcctttgc aacaagtaac tattctctta caaggcactc gtcatagtga
     1681 aagggaagat ataatcagtc aacttaagat tattctttcc agactaaaaa atggagaaaa
     1741 tacaggtttt gagcatgacg atgattttgg atattcattt cagtatgatg ccgcagcagt
     1801 aggaaaatca tcatttttcg atgaagcggc aggaagaaaa taagaataac gctctatcca
     1861 ttgcacaggc aaattaacgt taatctttta tgaaaatctt attaacaata tttatttatc
     1921 tgcttaccgc ttttcagtct gcaaatgcag gtggaatacc ttatacattt aaccagaaaa
     1981 ttcttcaggg gaaagttatt cgcgttctcg atggcgacac catagaaatt aaaacactac
     2041 ccgcaaaaat tgttgtgtat gaagttccga tccgagtccg attgataaac atcgatgccc
     2101 ctgaaaagaa acaacctttt ggacgctggt caactagtca gcttaaaact ctggttgcag
     2161 gtaaacaggt gacagtctct tattcacata aagaccgcta cggacgcatt atcggtcatg
     2221 tgttcacgac gaacggcacg gatgccagcc gctttatggt tcagtctggc gctgcatggg
     2281 tatatgaacg ttacaacgcg gatgagtcat taccggctct gcaacgggaa gcgcagaaac
     2341 aaaaacgcgg tttgtgggct gatacaaatc cggttccacc gtgggaatgg cgtatcagaa
     2401 attaaggggg caagaaaatg cattcttctt ttggtttgcc atgattctgt ctttttatct
     2461 ttgtgcaata ttaaaggctt ctttttaaca gggggcggca tgtatgtaat taatcaacag
     2521 acaagggaca ataatattct tacgttgcat gatgttttta aaatctatca aaatacaggg
     2581 gggcttcgtc ttgtaaaaac agaggagaat gaaaagtata tctccattat catttgtggc
     2641 gtttttcgta ttagaaaaaa caaaaagaat aacaaaatca ctttgtgggg atacaagcta
     2701 agagataagc acactggggt atggaccaga agaaattcat ttcctggtaa gattttttta
     2761 ttctggtcta aaaaatctgc atacgacatg gacgactggt taaaatatgc gattagctac
     2821 atcatcaaat cattgattga ggctggatat tcaattgaag acaaactcta tttgttccgc
     2881 gttggagatg aagaggaaca cagtgagagt cgttatgttt ccacactata tccactcagt
     2941 gtatataact cgtattctta tggaagatat ggcattattc attgccttgg taaaacagca
     3001 tcattcaact accccgataa cgtacaatat atcagcagac atgatttact cgaagagata
     3061 aaaaacaaaa tttatcacag agcattaaaa attatagggg tggataatga gcttaatgct
     3121 tcaaaagcat tgagcaaagc catatggata atggtagata aaaagatatt tgcacaatat
     3181 tccagagctt cacattgttc gatagcttac aattcaattc gtcaggcatt atttgaggac
     3241 tatctggatt tttcaaagcg aatccatatt ctggatgata tagatttttg cggattgtgg
     3301 cctatggttg gtcttctaag acaacaacat aaaaagggtc agtttatgct atcaggaaag
     3361 acaaaagagt tatatgaaag actttgtttt ccctatgagc ttagttatgg tgagttcaga
     3421 agtttacgac atgttagttt atcattagta tatgctttaa tttataacca tgataatgca
     3481 tcattcagat taacagtcag attattacgc catccactta tcaagaacta tcctgtaaga
     3541 gcaatttact ggattatcga ttatattagc attcgagctt acccagaaaa agaaaatgat
     3601 atttatcgta tatgctcaaa atggcttgaa tatcatcgtg atttatttaa aaacattggc
     3661 ttttatgatc gtaatactga atccaggcaa acatcacgct gggaaatgga aacgaatcag
     3721 ttgtgtcatg caattgactg gttgttagca gaagaacggt taattcataa aaaccaggag
     3781 tggccttctt tctggagact atctgatgaa tggacgcggc aagtgaagaa taacgttatt
     3841 cccgcaaccc aaaaatggaa aggaaccggg ataaactggc agaaaattga taactgtgta
     3901 aatgaattaa ttacatttga tgctctgtgt caggaaggtc aagagatgga acattgcgtt
     3961 gccagttatg cagactggtg tgcatctgga gaatatattg caatttcagt cctgatggat
     4021 aatgaaagag ctactcttgg actttcacga aaagaacatg atttaaccta tcagtttgac
     4081 cagatgagag gtattcgtaa tcaggctgtt tccagaaaca tgctaattaa aggcaggcat
     4141 attttaaaaa taatcaactc atcactaaaa ggataatgag tcatcgaatc tcattacagc
     4201 gccgatgatt gggcgtttat cctttctgaa tataaaaact ggctgaaaga taaggaaaga
     4261 aagttttctt acctgtttgt cttacgcaat ggtgaactgt ttcaccatga ggtcaacaaa
     4321 tcagagcttc tttgagaggt tttacatgca atatgcattg tttgatggat ttgaacgaaa
     4381 gtttttgctg gatgctcttg aattcggtgt tctgaaggac tggaaagaaa atccggtaaa
     4441 agaacttcct gatattgatg aatctgctca ccccttccat gtctgttatg gtggatattt
     4501 attaaatcct ggtgtttcag attcagatat tagcagaaaa ataaaagacc agacaggatt
     4561 ctggctggca gctattgatg atacccgcat ggattgtcat tcaatagctt attatgatat
     4621 tcacacactc cctctgattt cgtgtggtca tcaaaagata gttcctttcg ccgcgttaat
     4681 aaaagctgat gaatgcatca tttcaaaaat ttcttcgtat tctggttttg ctgtaacagc
     4741 ctttttgaga attaaagacc aggatattgc aactaatata cttaaccgtg agggaatttt
     4801 tgcctttaat ggctgtgagc acagattcag acaacctgta agtgaagata actggcaaca
     4861 ggcagtatca gaagaacgcg ctatccgttg tgccaaaaga ttaattcaat gtaaaggata
     4921 acaaaatgaa actttttatc gcagaaaaac ccgcagtagc gaatgatatt gttaaggcgc
     4981 ttggtggcaa ttttacccgt catgatggct ggtttgaaag tgataatact attgtgacta
     5041 actgttttgg tcatattatc gaatcacagc cccccgaaaa ctataatcct gaatacaaag
     5101 agtggaagat tgaaacactc cctttgcgtc tttatcccgt aaaataccag ccagtagaaa
     5161 gcgccgcaaa acaagttaaa acgattattg agcttatcag gcgtgccgat gtgaccgaaa
     5221 ttattcatgc tggcgatcca gatgacgaag gacagctact tgtcgatgag gtcctggaat
     5281 atgcaggcaa cacaaaaccc gttaagcgcg ttctgatcaa cgacaacacc cttccggcag
     5341 tgaaaaaggc actggcaaat cccaaaaata accgtgactt cagaggactc tatctcaagg
     5401 ctctggcgcg ttctgtagcc gacgcgatat acggtttatc catgacgcgt gcttacacaa
     5461 tcccggcaaa aactaaaggc tataaaggtg ttttgtctgt cggacgggtc cagacacccg
     5521 tgcttggcct gattgtaaat cgcacccgtg ccaataaaaa tcataaatcc agcttctact
     5581 acaccatgac cggacatttt caacgcggcg ctgatgttat cagggcgaac tggaaacctg
     5641 gtgagttcgc accgctgaca gaccgcaaat tgcttgataa aacctgggcg aatggaacag
     5701 caacatcact tgcaggaaaa ccagccacag ttgaagcggc agcaacagat gataaaaaaa
     5761 cggctgcacc gttgcccttt aacctggtac gacttcagca gtacatgaac aaaaaattca
     5821 aaatgactgc tcaaaaaacg ctggatatca cacagcagct acgcgaaaaa tacaaagcaa
     5881 ttacttacaa ccgttcagat tgttcatatc tttcagacga acaattcagt gaagcaccac
     5941 aaataatcga tgccctgaag tcggtgtttg atcagccaat ggatattgat acgacacgta
     6001 aaagcaaggc atttaacagc gcaaaagtaa cagcgcatac agccatcatc ccaaccgtta
     6061 gcgtaccgga cgtaaacgca ctgagcaccg acgaacgcaa cgtttacctg gcaattgcac
     6121 aacactatct tgtccagttc atgcctgaaa aggcatatca ggaagtatcc gtcgccattc
     6181 agtgtggtga tgagtcgttt tatgcccgtg ccaggaaaac tacagacagc ggatttgaag
     6241 cgtttcttgg tgtggaaaat gccggggacg atgaagcaga agtcgaaaat gatgattccg
     6301 cttttgatct tctctgtaaa attcgcacag gagaaacagt gacgacaaaa gaagtggttg
     6361 tgaatgagaa gaaaacaacg ccaccgccat tattcaccga agccacttta cttgccgcgc
     6421 ttgttcgtgt cgcagatttt gtcactgatc cagtgattaa aaagttactg aaggataaag
     6481 atcgggataa aaaagacgaa catggcggta ttggtacgcc agctacgcgg gcttccatat
     6541 tggaaacact gaaaaaacgg aactatatca ccctggaaaa aggaaaactt attccgacag
     6601 ataccgggta tgcgcttatt gatgctctgc cggatatagc tgttaatcca gatatgacgg
     6661 cattatgggc ggaaaaacag acactcattg aaaacggaga aatgaccatc gaacagttcg
     6721 ttgacgaact gtacaatgat ctgataccga tgatcagtaa tgcgaacagt gctgaaataa
     6781 aagtttcccc cagcgcgccg tctggtcagt cacaacgcct gtcctctcct tgcccttcct
     6841 gtggtaaaca gattgttatc aggccaaaaa gctattcctg tacaggatgt gaatttaaaa
     6901 tctggaatga gttttctggc aagaaaatca cccaggcaca ggccgaaaaa cttattaaat
     6961 caggaaaaac cgatttgatt aagggattta aaaagaaaag cggtggaacg tatgatgcgg
     7021 ttcttgtcct tgaggataag aaaacaggga agctgggttt tccggcaagg gctaagaagt
     7081 gaaagcaaag cagaaatggc ttttcaggtc aggaattgct tcagttaata gccaagtctg
     7141 gtcgtgggcg agcgccaaaa ccgattgctg aagcactggc aggaggtcgt tcccttgatg
     7201 agtttctcat cgagaaatga ttctacagcc ctccttttct ctggagggcg ttatcctttg
     7261 aaatatccgt ttattttgat gataattctc atgtttcgtt tctcggcgtg gagagtacag
     7321 attttatggt gaatggtttg ttgcttttcg ctgttagtgt attagttttt ttagtgataa
     7381 tcctgatgtt acatttgaga acagccagtc ttctttctga atgctctgaa tatcgcacga
     7441 aatgtgataa tctctatcat ctatgccaga tgcaagagaa agagttagaa catctacgtg
     7501 cactcattga tgcgcataac atagattatt agacgttgct ttagggggat ggataaatga
     7561 aatttattat tttaaaaaag gataaagata gcacaattgc cagaaacgac tctgttttcc
     7621 ctgttaacaa tttctttgtc gttttaacta ccgtgtcttc cgtggctaac tctttcggaa
     7681 tagtgtcttc cctggctaaa tctatcggaa tatcttttcc cacgggcaat gttattgaga
     7741 cgtgtttttt cggtatgttt tcactattac tggctgcttt cagttttgta agtacaatgg
     7801 ggctggcgaa cgcagtcccc cccattaaaa atgtgtttcc gcttttgttt ttcgttcttg
     7861 tcttaattac taattataat atttacttcc gataagaacg ggggcgcggc agcggctttt
     7921 cttctgcccg ttctcccgtc aataaaccca gcagtagaaa tcaaacatcg ttgtagctat
     7981 tatagggcaa ggatgttctt caggaattgt acaatgaaaa caaaacaaga ctggctcttt
     8041 cagttaagac gttgctcaac actcgacaca cttgaaaaga ttattgaaaa aaaccaaaac
     8101 tgcctccccc cttctgagct tgagtcgttt aatgccgccg ccgatcacag gcttgcggaa
     8161 ctcacaatga ataaactcta cgataaagtc cctccatccg tctggaaata tgtatattag
     8221 ttttcatcta ttaatattag atttttgatc gacacaaaca ttagcaatcc ctatacacct
     8281 ttcatagaat gaattgaacc ctgtatatag cctgtctgaa atggatatct ggcaggctat
     8341 cttaatagtg ttataggtga tgtgaatgtt tttccacatg atcactcttt ttctgttctt
     8401 atcactattg atactcctga taaggatatt ctccgccaga ctaataattc cggctgtggg
     8461 cgtgtttctg ccagtatcat cactctggat gggatttcat atgctcacac aaaatgaatc
     8521 ctgtctggat aacatcgcct gtgttgctcc cccatttggt cagttcagta cccttatagg
     8581 atggtttttg attgcggaat caatattaac cgcatgtgct ggcgtatatt ttctccgtaa
     8641 aaataaaaaa cgctggtcac gtcatgatac agtcatgttt ctttttgcaa gtggcctggc
     8701 aattttcagt acgtggatgc tggaacacca atgggtttga acttcccttg acagttttgt
     8761 tggttcttat acttatagac tggattaaat catgaaagaa tacgaaaaag cggctttaat
     8821 atattcctta aaggaaagca atacattagt tcttcggatt atactaatct ctattttagt
     8881 tgtcggggtg atttcaggcg cgttatactt atataactta gcgatctcgt tgtttgattc
     8941 aaacccgacg tctgtgcaat tcaccttttg cggagtaggc tgttttattg ctctattact
     9001 tcaatggata tctaatgttg atgcaaatta cagagaaaga attggggatc aaccaggtac
     9061 aataaaaaac attgtacaat atttatttgc agccattatt accattagtt tcattgcaat
     9121 ggcattaaca gccatattag tcttaaaaga ctcgtccaac atatggctca acattacttt
     9181 tattgcatta ttactgtcca tttttgtagc tattatttct gtttggggtt gacttttttt
     9241 cttaaaaagc ccgaaaaccg ggcttttttc ttaattagtg catgtcctgt gaatcgtcac
     9301 gctgctgctg cgattcctga tgatcttctc tggcctggac aaattgtttt ccaagagcat
     9361 cttctgccat ttcacgagtg aatccgtgat taagcaaatc ggattcggcc ttctcccttt
     9421 cgaacttcag tccttcccct ctgaacttgt cctcaaagcg atcaattgcc tcaagaatct
     9481 tgccgtcttc cggcgtgcaa tcccgtccct gtgctttctc aatttcatga cgctccacgc
     9541 gttcagcatt tttcattaca gcgtcaatcc cctgagaaat atgttcagac tcgacgcgat
     9601 aaatgacagt atcgcgctta ttgagatcat gtagctcgca ttcaacccgg ccccactcaa
     9661 tagcatcctt gagagagctg aaatctttta tttcagcatt cagaccttca tgtgttgaga
     9721 aatagacata atccttctgt tgttccagtt tattcacatt ttcaatagcc cttgtactga
     9781 aatggatacc tgtaattacc ggaacacgag ccgatgtttg gtcccccgtt aaaacttcag
     9841 aaggtgtttt tccagtgatc gcggcaaatt gctgctccgt aacatccact ctggcaactg
     9901 catcaccacg ctctaagcca tgcttaccag cgaccgcaag gtgataaaga ccaccttccc
     9961 cccgggtaat ttccgcctcc acaacgatat ccccattgca ggaactgaac tgagaacggt
    10021 ctttaaccag gaagttttca agcccggaga tcatatgctc gttagctgaa atactggcga
    10081 gtatctcttc tggtgattct ctggacgaat cccccgcctg ttccatcact gccatattag
    10141 cgtcattaac agcgtcctta attcctttaa aggcgatttc ttctcttgtt acaggtgtga
    10201 ttaagatatc atcctggtta agcccgtgct cctgaagcat cttattttta tattcagatg
    10261 cgtcgtgcaa tgactcaaaa gtcttaatat cttcactaaa ctcatccttc gaaaaagaaa
    10321 ccgcatacac tggagtacgg tattcttcat atgaattggc aattatttca aatggcctat
    10381 caaacgttac taatgccccc tctggataag aggataattc atcggttgag acagttttag
    10441 acataagcac aacagtatca aaaccatcac catttaaata attgctttca tatttttcta
    10501 ttgcagaaga tttgctttcc tcataacact gaatagccgc atctttagaa tgaaagacga
    10561 taccttcatt caaatcatgg aagcgactaa agtttataca ataaatttcc tgtttattat
    10621 gcacctgcgc agtagcctga tcgtgttgag tagtaagctg ctcctgaatc agagcctgtg
    10681 ttctgtcaat acctaactca ttcacaattt tgcaaaattc ttttgcgtcg ttttcattat
    10741 caaaactggc aacgtcaaaa atatcatcgc tgtctccagg tacatgtccc agcacttccc
    10801 aaatctcatt ttcatcgcta tccagagatt tcaaatagcc aaattcacca tttgtgcctt
    10861 tactaagcat ctgtgaatcc agggtgtttg ccctggtaat aacaccagga tcatatgaag
    10921 atgtggcctg ctctgcgacc tgtgcttgct ccgcgacctg tgcttgctcc gcgacctgtg
    10981 cttgctccgc gacttgtgct tgctcggtgg cctgtgcttg ctcggtggcc tgtgcttgct
    11041 cggtggcctg tgcttgctct gtggtctgtg cctgttcggt ggcctgtgct tgctctgtgg
    11101 tctgtgcctg ttcggtggcc tgttccttca atgcatcacc tttctctatt ctggattcgg
    11161 actcctggct attttcctgt gtttccacct gctcaaactt attaccctga agttctgctt
    11221 ttaattccag catcatctgt tgatactgct ccgggcgtaa aataatcttc atgtcctgct
    11281 ctgcggcaac ttcagcgcac atttttttga aatcttcaga gccggtaagt ttgagtacac
    11341 caccaaactt ttccctggaa tactcaagca ttattccgat ctcttctttt gagggatttt
    11401 tacccgcaac gacaaagttc cctttgtcgg taaagacagg cttttccgat tcctgatcgc
    11461 gataaacaat cctggaatcc tgcttatcca tcacaagatc ttttagtttg gtgttaacct
    11521 ttttcgcctc ttctaagcgg cgctgggaat ccaccgcttt ctgaagcgaa agttcgggtt
    11581 cgtcagcaga aatggcgttg atttcatcga agttgagata gcttttgaga cgttcaaggg
    11641 ctttcatttc atctttatcc ttatgccagt cagcatgata ttgacgaata atgtgtcgcc
    11701 catctgtgtc gagttcagaa agacgctcaa gtgtggttag ttttttgtaa acaatcacac
    11761 ccacggcaat atcacgctgt tcacgtggta ttaacttcaa ctcgcgtctc atctctcgca
    11821 gatcattata cagcttgcca cgctcaaacc tgatagtatt tcgtaatgtt ttttcagata
    11881 aattcagtga gttaagacgc tcacgagctt catgatgcgt aaggcgtctg taggttggat
    11941 agcgcgtata tggcgtgttt tctaactgct gtgaataaac ttcaagcaga aaggatttcg
    12001 catctttcca ggcaagatta atatgctttg tcagaaaatc agatgcgttc atattacgat
    12061 tccccacaga aaaccggggg gaaccgtcct ttgctgttct gattttatgc tgcgctggaa
    12121 taatattaaa acgctcggcg gcagaagaca gaaacctgac aggatcaata tttctgcgaa
    12181 tttctgccat cgttgtaatt tcgtttttct ctgccgcctc attcagagtc tgaaacatcg
    12241 tttcacacaa aaaagaagag cgttcaagac tttttattcc tgactctgtg aattgtctat
    12301 cataatcccg tcgcacatcc tgaccaggat gttctctttg cggtaattgc tctgccagat
    12361 cacgctgcga aatatctgac aatacactga cagagtcggg tcttgtgaat ccattgattc
    12421 cgtaaaccat accgcgttgg ggcatacgtg acaatccgac tgttgcttca gattgacgga
    12481 ttctggcaaa ctttttagga cacgacttgt aacctccctc tcgtcctgac gtttgctgag
    12541 aatttctttc gttaagtttt tctcttgagt catattcttt tatcctctct tgcaggaaat
    12601 caatttgttg tgaagggctt aatgtcttgt aaagttccct tgttttctcc gcctggttaa
    12661 aaatatgctt aatctcatag cttgttttat ttaaccaggt attaagtctc ttttctatct
    12721 ctttgtgtgt tggtttctca agtgttaatg tccttgtctc aatgtaattc tgacgaaaaa
    12781 gaggactttt taagtttgtg tattttttat ctcccggcaa ttttacagcc agatactcat
    12841 tggttttacc agcatttctt atacgaagtt caccatactc tgcaacaata tctttgaata
    12901 cagagtaatt ttcaattttc tcgttctcta tacgcgaaag caactcacct ttcaattcag
    12961 agtgatgctc tttgtaaaga tctcccttaa ctctacttat aatttttccg taatccgcat
    13021 ctttacgcgg atagtcttta gggctatcaa gattatattt attattaata aattcctgta
    13081 tagcatcaag ctgttcaatt gtatgccctt tggtaacatc tcccactgga tttaggaact
    13141 tttcagtaat caaatttact ttaggaatta caatatgaat atgtggtttt ctttcaacaa
    13201 gttctccggt gctattatcc tggatatgac gaattttagg caaatgagct tccgcataga
    13261 aagagtattc atcaggatga taagcattca tcaataactt tttataatca tcaacaatag
    13321 cctttaacac ttcattcgac acatgacttt catgaaaaga cagagtaatg tgcaaataac
    13381 gttcctgact tttgttttca atagaatcaa taatcttatc tgtagtctgt aaatcaccat
    13441 caagaacgac acgatgatca agttcatcac gcgtgtactg gcgttctgct ttgcgaccat
    13501 ttatcagata atcaacgata ccatcattac caccaccgta tctgacaatc atttagctat
    13561 ctcctgaagg cgatcacgaa taccgattag tccatttaaa acctttttat aaagcgattc
    13621 tgatataata ccttttaaat gatctgaatt taatcggtgc gctatttgat ttatgttgtt
    13681 tcctgcccaa ctcaactgag aaagacaacg tttgtattct gggatgttac gtttatcaga
    13741 tgcatatgtg tttattttac caatagtaag cagcctgaaa aattctgact tactaatatt
    13801 aagctctttt atagccctat cgaacggagc atattcttct tcggtaagtc tgaatgttac
    13861 atgaacgttt ttgttggttt ttttcttcat atgtcctcaa ggttcgtaga accgcataag
    13921 aacggctgcg tcgttcgggg tttcaaaggg gcatccccct ttggcacacg attgtaacat
    13981 gaccggaacg gtcttgtgta caatcggtat cgtgcctgtg ttttagaaga acaaaaaata
    14041 ccgtgcatac agcccacaca atgactcaca taatcaccca caactcaaaa cacacaaccc
    14101 acacaatcac ctacacttta acccacaaaa aagaccacaa ccccattgac aacccacaaa
    14161 acaacccaca taatcaccca cacaaacggc gagcaaattg ctttaaataa ggtattatgc
    14221 caatgtagga ggtagcgaga gagagaggaa atgtaacaaa tgaaaaagat cgagagttta
    14281 ttatctccaa tatgcgaggc tataaacgaa acaaaatcga aatcaaaaca agctgtcttc
    14341 aatatgtatt tgcaaaatat acttgaagca aagaaatatt atacatggga gcaaatagct
    14401 gattacataa acaacaacac caagacagaa ctagcccaaa agacttatat aaacatgaca
    14461 gagagggcta aaaaaagaca aaaatgctct gaaccaaaag aacaggcaaa aaccatagat
    14521 gtaattgatc agaatgaagc taaagacgat gaaccgagag ggggggtaaa aggttttttt
    14581 tcaaacatta gtgagagcaa aaaagaaata gagcacgatc caagtgctac gaatgaaaaa
    14641 ttcataaaca aatatcttta agtaaaggag cattataaat gaaaaactca atcaatttca
    14701 tccttcaggg taaaggtggt gttggaaaat catttgcgac cgcaatactg gcacaatact
    14761 ttattgatga aaatcacatg gataatatcg tagtcggtga cacagacccg gttaatacaa
    14821 ctacggtaaa agtaaaaaga ttaaatgcag atttgataca aatcactgaa aacagcaaag
    14881 taatacaatc caaattcgat ccaatgttcg aatcaatgct gaccaatagt cagaatacct
    14941 ttgttattga caatggcgca tctacatttt taccgttaat tcagtatttc aatgataact
    15001 gtgttatgga catgtttgaa gatgtagaac aagacgttta tatccatact gttatcgttg
    15061 gtggacaagc tcttgcggat accttgcagg gatttgaaga actgaaagaa ctggttaaag
    15121 gaagtaaggt aaaacttatt gtctggataa atgaatttca gggtattcca gcacttgaaa
    15181 atatcccatt aatagaaact aaattcattg aaaaaaacaa ggatgtaatc gctggcgttg
    15241 tggttattca ggacagaaaa agtgatgcct ttgcctctga catcaaagaa ctaacagaaa
    15301 aatcactaac actgaaagaa gctattgaat ccgaccattt tggattaatg gctaagtcaa
    15361 gattaaaacg agtcttcaat gacatttaca aacaattaaa tagcatctat gacgatgaac
    15421 acggtgaata atatgagtac agttaatcag ccagaattaa aacagcctga aaaggctgtt
    15481 tccagtgaag atattgataa ttttattgtt gatgtattca aggagacagg gcataaaata
    15541 tcaaaagatg atccggttat ttcattaatt tttctgaacc aaaagataca ggaaaaattc
    15601 agcaatgagt tacaagctaa cttcacagcc ctgtctgagg gcttcagaca ggttgtttca
    15661 tctgtagaaa atgactatat tcaaagattc aagaatatcg ttgagacgtg cggcgatctg
    15721 gataatgaaa taaaagaaaa agtagaagag ggaaaaaacg atctaaaaga aacaagtgtt
    15781 gaggtaaaag aaaaattaac agatgatatt attgaactca tatctggaat taaacgcaac
    15841 caggaaaaaa acaacaaatt atatgaagaa aagttgaagt cattatctaa ggctgtaaag
    15901 cccttcagta caagaacagc cattgcaatt tgtgccttgt gcacattatg cctgtctgcg
    15961 gcattttctg gcgcaacctg gtatgttgca caacatgaaa aagaggcaag cctgcgcttc
    16021 tatgcaagag cttttcttga tatgaaaaaa ataactgaag agagcatgag gaaattacca
    16081 aaatcagacc agcaaaatat aaaactcaaa ctagaagaaa ttgattcaag aaagccataa
    16141 atatgaaaag gagtccctaa tgaccacttt taccgacgag gacaaagaac taattaaaga
    16201 aatcagagag cgaattggca gcctggacgt gcgagacaat attgagcgcc gggcttatga
    16261 aattgctctg gcatcgctgg aagccggggt tgttgcatgg aggtatcgtt acgtgaaaaa
    16321 agacgttacg gactctcagg gtaaaacgtg ggttggtgac tggaaatacg taccgacaaa
    16381 agaagactgc aacgacaggc cgaactatga aattcaggaa ctctacaact taccacctgc
    16441 aatagcagca ccaacagtac caagaggact agtaaacgca gtgcgtttct atgaactggt
    16501 aaagagtgag aatccgccag ccgaaaccgg agcatggaaa gacgctgttg actgggtgct
    16561 caaagaggct tgccaggctg taaacattgg catcaaagga gagtgagaat gcaaatttca
    16621 ccggttactc ttcgtgttgc gaaggcgttt atatccagac atcaccgaca caataaacca
    16681 cctgttgggc ataaattcag cattggtctg agaaatgatg ccggagaatt gataggtgtg
    16741 gcgacagctg gtagacctgt tgcacgacat ttggacgatg gattaacact tgaagtaaat
    16801 cgcacatgta ccacaggaga acgcaacgct aacagcgcgc tttatggtgc tgtctggcgg
    16861 gcagcaaaag ctatgggtta tcaacgttgt attacgtaca cccaggcaga tgaatcagga
    16921 gcatctcttc gcgcagctgg ttttgttcgt gtgaaagagc ttcctccaag aaaaagctgg
    16981 gcggaatcaa gcgtcgccct gcggagtaaa cgcgatccgg tcggaaacgg tggtgttcct
    17041 cgtgtgcttt gggaaatcag gagaatgagt accactggca ttcgcatcaa aggagagtga
    17101 tatggcaact ttgcaggaat taatcgacct gacgccagaa caggaaaaag cgtggaatcg
    17161 ccttgtgaag gctgtaaagg atttcagggc agccggagga aagttttata gcgtcctgga
    17221 cacgctgagc gcatacaacg gcgagcacgt tgccagcatt gataacgata agggctacca
    17281 cactgcaagc gtctatatgc ctagcattga tgcgccaggg ctaaccagtt gggctgatga
    17341 ttggcacggc atcacgctga aagatggcgt tgaagtggat gaggactaac acatgacaac
    17401 gttcaccgac aaagaactga ttaaagaaat caaagagcgt ataggcagcc tggacgtccg
    17461 agacaatatt gagcgtcggg cttatgaaat cgcactggca tcgctggaag cagaagcgcc
    17521 aaatttgcct ggtggtttca caattgaaga agcaaaagaa ttacacgaaa atctggtaaa
    17581 aagtcacgta agcaaggctt taagtggcga aaaaatgaaa aaagaaaatc gtgatgccaa
    17641 tttgcgctgg attcatggcg taatagttca ggctgcgtgg tttgtgaaag catcactagg
    17701 gcagaacgca tagtgcgtac ctgcttttct cctggcaacg gtggatcttc cgcttcctcg
    17761 tcgctcgctc gctatgctcg gtcgctggac tgcggcgagc ggttagctat caaaaaaaat
    17821 ccccgcacaa cgcggggata gttttattta tatgcaagtt catgacgtgc cgcctgagcc
    17881 agaaaatggc tacgatcttt gtatacgcca ccactttctc tcacatatcc atcaatacga
    17941 cgaatcaata catcaggcaa tgaaatatta atacgttgtt gtttgccctc aatttctgac
    18001 aaatcaacat caattacaaa ccagctatca cagtggttat aatttgggtt ggcggaataa
    18061 gtcaaacacc cttcatcatg aatatcatca acagaatgag caccgcttat taccatctct
    18121 tccacgattg ccaatatcgc ttcacgcgcc attgcgggta tttctgcctg agaatcagca
    18181 gcagaaacac atccaaaatc aaagcaatca aaaaccggaa cggtgatacc gtatgctgta
    18241 tgatcatctt tcggtgtttc gacaccaaca gaaaagaaca tggctacctc caaagtcggc
    18301 ggggattaaa tccccgccat ttttttgatc gatctgacag tacctagcgg caagtccttt
    18361 ttaggatgag gaacaggaaa agtttttcct gttattggcg accaccatat ctggtgactg
    18421 cttgccttat gccgtttcaa ctcgcaacca gcagctatca gttctttcaa caggtcagca
    18481 gatttcatat gtcctcccta acctgaggat aattatacac acgcatacac acaaaggcaa
    18541 attatttcat tcgtatgact tatgaaccac actgtaaaga cagctcaatc tttacagtgt
    18601 aagtcattta tccagtttta tcgctgtgta tgtgatacat agaaaataat atagtatttt
    18661 actatatgat atctgattga cacttttaac tgcacatctc atcatttaca ttgtaaagac
    18721 aaacatagat ttacaatgta aagctatgta attaaacact ccgtgtccca aataaggaaa
    18781 gaaaatgact caactaatcc cagaaaagat actggaaatc atagatgatc atgatcgtgc
    18841 agaaaaaaag caacgcaaca aaatcggttt catttattta tgtctctgtt tagcaataat
    18901 tggtgtggcc gcgtatacct ttatatcaac gtttagcatt aatgtggtaa caatcatcat
    18961 aatgcttttc ggtgtgttta tgattgtgat ttttaagagc tatttagact catggagtgg
    19021 gataattctt agcagtgacc acatattgtc catactcgat aaaaccaaag attacccaga
    19081 aattaaacgt atagttatta atcgtctgct ctccggctca atactcacgg gtaaagatga
    19141 agagtacata tacagccaac tggaaaaggc ccaacaaagc aacgaacgtg aaaagagatt
    19201 gcaagcaatt aaagagtata catcgtaata caacgcatta cgtcgaaata ccctggatca
    19261 tcgaagccac atacccctaa gcgcagcgca accgccggaa aacgcaccac actgtaaaga
    19321 caataaaatc tttacaacgt aaagctaact atgtaataca acgcgctaca caataagaaa
    19381 gggagagcat gacactctcc ccatatatga ctcaaactaa agcattatcc cggttatgcg
    19441 cccaccaaat gatttcgcta ataatccatg cttctatcgc gggtgttttt acaggttccc
    19501 catttacaat gcggtttgca agctctatca tttcacatac tgacatttga gaatctccaa
    19561 agatgatctg tcatttggta acaggctgcc ccacagcctg ttaccaaccc caaattaata
    19621 tttgctgatt actttaccct ccttaatcat ttttattagt tcctgttcag aatattcttt
    19681 tccgtgttcc aatgtggaat atatattgcc aatagtccat accccacact ttttaatttc
    19741 tgcatttagc caggattcat gcagaataaa caacggttcg caatcatttc ctttttcata
    19801 aacataaata gattcggtat catttccaga atcagaagta ccaacgactt tttctatatc
    19861 gaaatgtttt tttagaaaag ttgtgaaatg cattttcatg ctcctgccgc tgagcggctt
    19921 tggtggttgg ggaccatccc ctcccaatgt gtttataata ggtcagaact gacctattat
    19981 caagtatttt tccctccatt cctcaaaaaa aaaacactat caaatacaga cagttacatt
    20041 catgtaatgc ccgacagggc atggaagggg cgaaagaaaa taccagggag cagcggggcc
    20101 acatcgcccc gaagcgcggc gcagccgctg acgaacatac caggatgcac agggccatcc
    20161 cttcgccctg caaagccaga acagaccaac aggccatgat catgctgatt tgcgccgcag
    20221 aatgccgcag ttagcgcgag cgacagcgag tttacgccgt cctggtgcat tgcagcaggg
    20281 cggggcgctg tactgcgcgt gtgagcgtca gcgagctacg ggcgcaattg ctcattgttc
    20341 aatgcaactg gggctattaa gtccagccat aaattcagtt atggtttttg tagcttattc
    20401 cgtgatttcg tcatacgtta attctatttt catcttaata ttccttaatt acttccagaa
    20461 tcagaattct ttttattcct tttacaaagt acataatcag ggtgttctcc agctaaaagc
    20521 agaagaaatt taaactctac tggtgtaagt gctgtaccgc ttgcagttcc gacaggttct
    20581 tctttgcgac gccaggtacg cggactcatg ctaaatattt cggcacattc tttctgtgtc
    20641 aggccaattt tttgccgcag acgtttaatg ttttctggtg tgttttcatt tggcattttt
    20701 tccctcactc atttcctgaa tcatcataga aacaatttct ttatcattct ctcttaatcc
    20761 gtcgctggca aagtcgatag tgtcgaatgc cttctcatga aaaacaagat cggcaacagt
    20821 tttcaccgtt gctttcgttt ttcctaaagt cgcagactta gaaaaagttt ttactcgctt
    20881 caccagatga aacgtatcac cttctttatt cagataatat tgtctgtcac cttttcttat
    20941 cttcatattg agatagtctc cccttttaac agtaaagggg catatagccc ctttctttta
    21001 actttttccg gtaatcaaat cgacatcttc ctgataaagc tccccaagaa ccggaacaac
    21061 gcctttttta taatcacgga tattatggca gtctgtaaaa atgacgacat aagcctcgcc
    21121 tgttttttca tgtttaaggc aaattgtgca aagcgtcacg cgcacaacag aaaacacatg
    21181 accagttgta gagacgatag caggaaaagt agattgtttc atgtttaact cctgccgctg
    21241 agcggctttg gcgattgggg accatcccct ctcaatgcat taataatagg tcacaaatga
    21301 cctattatca agtattttct agacgagctt tattgaaaaa tcattttcaa taaattcatc
    21361 taacatcttt aatataccag ctttatttac gaaatcatcc tccataattt cgatacttgc
    21421 tgatatttca catgtaaact caatgctcct gtcaaatccc gcaatcc
//
LOCUS       DAZFTN010000082        20595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFTN010000082 DAZFTN010000000
VERSION     DAZFTN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..114
                     /locus_tag="ACHKV2_003394"
     CDS             <1..114
                     /locus_tag="ACHKV2_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO6941221.1"
                     /translation="WYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(174..680)
                     /gene="yciE"
                     /locus_tag="ACHKV2_003395"
     CDS             complement(174..680)
                     /gene="yciE"
                     /locus_tag="ACHKV2_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6941222.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(726..1226)
                     /gene="yciF"
                     /locus_tag="ACHKV2_003396"
     CDS             complement(726..1226)
                     /gene="yciF"
                     /locus_tag="ACHKV2_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6941223.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1312..1491)
                     /gene="yciG"
                     /locus_tag="ACHKV2_003397"
     CDS             complement(1312..1491)
                     /gene="yciG"
                     /locus_tag="ACHKV2_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6941224.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1872..2678)
                     /gene="trpA"
                     /locus_tag="ACHKV2_003398"
     CDS             complement(1872..2678)
                     /gene="trpA"
                     /locus_tag="ACHKV2_003398"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO6941225.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(2678..3871)
                     /gene="trpB"
                     /locus_tag="ACHKV2_003399"
     CDS             complement(2678..3871)
                     /gene="trpB"
                     /locus_tag="ACHKV2_003399"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO6941226.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3883..5244)
                     /gene="trpCF"
                     /locus_tag="ACHKV2_003400"
     CDS             complement(3883..5244)
                     /gene="trpCF"
                     /locus_tag="ACHKV2_003400"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO6941227.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5245..6840)
                     /gene="trpD"
                     /locus_tag="ACHKV2_003401"
     CDS             complement(5245..6840)
                     /gene="trpD"
                     /locus_tag="ACHKV2_003401"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO6941228.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6840..8402)
                     /gene="trpE"
                     /locus_tag="ACHKV2_003402"
     CDS             complement(6840..8402)
                     /gene="trpE"
                     /locus_tag="ACHKV2_003402"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO6941229.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8424..8517)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(8494..8538)
                     /gene="trpL"
                     /locus_tag="ACHKV2_003403"
     CDS             complement(8494..8538)
                     /gene="trpL"
                     /locus_tag="ACHKV2_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO6941230.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8676..9557
                     /gene="rnm"
                     /locus_tag="ACHKV2_003404"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             8676..9557
                     /gene="rnm"
                     /locus_tag="ACHKV2_003404"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO6941231.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9554..10174
                     /gene="yciO"
                     /locus_tag="ACHKV2_003405"
     CDS             9554..10174
                     /gene="yciO"
                     /locus_tag="ACHKV2_003405"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO6941232.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10202..11785
                     /locus_tag="ACHKV2_003406"
     CDS             10202..11785
                     /locus_tag="ACHKV2_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO6941233.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            11998..12870
                     /gene="rluB"
                     /locus_tag="ACHKV2_003407"
     CDS             11998..12870
                     /gene="rluB"
                     /locus_tag="ACHKV2_003407"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO6941234.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12910..13500)
                     /gene="cobO"
                     /locus_tag="ACHKV2_003408"
     CDS             complement(12910..13500)
                     /gene="cobO"
                     /locus_tag="ACHKV2_003408"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6941235.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(13497..14255)
                     /gene="yciK"
                     /locus_tag="ACHKV2_003409"
     CDS             complement(13497..14255)
                     /gene="yciK"
                     /locus_tag="ACHKV2_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO6941236.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            14475..15524
                     /gene="sohB"
                     /locus_tag="ACHKV2_003410"
     CDS             14475..15524
                     /gene="sohB"
                     /locus_tag="ACHKV2_003410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO6941237.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(15560..15811)
                     /gene="yciN"
                     /locus_tag="ACHKV2_003411"
     CDS             complement(15560..15811)
                     /gene="yciN"
                     /locus_tag="ACHKV2_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO6941238.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            16191..18788
                     /gene="topA"
                     /locus_tag="ACHKV2_003412"
     CDS             16191..18788
                     /gene="topA"
                     /locus_tag="ACHKV2_003412"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO6941239.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            18998..19972
                     /gene="cysB"
                     /locus_tag="ACHKV2_003413"
     CDS             18998..19972
                     /gene="cysB"
                     /locus_tag="ACHKV2_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO6941240.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            20291..20431
                     /gene="ymiA"
                     /locus_tag="ACHKV2_003414"
     CDS             20291..20431
                     /gene="ymiA"
                     /locus_tag="ACHKV2_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO6941241.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            20434..>20595
                     /gene="yciX"
                     /locus_tag="ACHKV2_003415"
     CDS             20434..>20595
                     /gene="yciX"
                     /locus_tag="ACHKV2_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO6941242.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPK"
BASE COUNT         5036 a         5028 c         5333 g         5198 t
ORIGIN      
        1 tggtacatgg atatcgatac caccgccaat tataaactgg gcggtgcaca gcaacatgat
       61 agcgtacgcc tcgatccgtg ggtgtttatg ttctcagcag gatatcgttt ttaattccgc
      121 acaaaaacga ccccgtaata tacggggtca ataaggacat ggcataaagg gtattatttc
      181 ttcgcttcta cgccatcagt ttcagagcga attaaaaatt tctcagttgt ttgcggaata
      241 tgctgaatca gccagtcggc catttgcttt tcctcattta aaatcgcttc gatgatcgga
      301 attgaggctg tatcaccggc attttttgct gctgctaata gagaggtgta acaggcgatt
      361 tcaaattgct cgaagacata tccgctaata gagcctttga ctatttcatc agaagggaat
      421 ataccaccga ttgactgccc aagcgcagcc attttactca tggaatcttt aatgactgaa
      481 cgtgaaatgt cattacgatc aagaatagtt tccagttgaa caatctggtt tttggtttca
      541 ctaagatgtt gttcaatacg agcacgtagt tcaggataat tatctatacg gctggccatg
      601 gattcaagca tagattcggc ttgcttttcc attgcatggg cgtcacgtaa ccagtcatga
      661 taatgttcaa tacgattcat gaaaatgtcc tctttataaa atgaccaaaa gttaaagttc
      721 atatttcagg ctttattttc ggctttctta tttacgttat taagggccag atcagtcagt
      781 ttgatgtcgg tggccttttc ttcttccagg gtctctttca gaagcttcgc tgctttacgg
      841 taacctaatt gttcagccag cgtcgctaat gtcccgtaac tggcaatctc ataatgctcg
      901 actttctgtg ctgcggcaat cagtgcggca tcacgcactt cgtttttctc ggtactttcg
      961 atgacctcat tagcttcttc aataagacct tccattgcca cacatttcat gcgcttaatt
     1021 ttcagattcg attctgattc cacaacttga tcaatacgtt caatctgtcc atgtgtttcc
     1081 tcgaggtgcg catgaaaagc ctgacttaat ttttcgtttg atgttgctct tgcgagtttt
     1141 gccagtgccc gggttaattg tttttctgcg ctgtaggtat ctgaaagcag gtgaataaat
     1201 acatcttcaa tggtcttcat attcatattt ttctccagtg aaatcactgc gagccatacg
     1261 gcccgcagat gcaattatct ttatcagctg atattaataa ttaacggtga atcaggattt
     1321 tcctgattta ttaccaccgc tttgttgacc gcctttttta cccgcttcag atgcgcgttg
     1381 cggatcattt ttaaaattac cgccgctatg ctgaccgcct ttacggcctg cgtcggatgc
     1441 cttctcacgg tcttcggcga aatttcctga accaccacga tgttcggcca tgttatttct
     1501 cccgttgcgt tgcattgttt cattaatatg agtgttgtgt gtcgatactc attaaaatta
     1561 gtcgctaatg aggattagtc aaattaagcg taacgagaag aaagtaggaa aatatattat
     1621 gacgaacgtt cttgatatat taacaattgc ctgcggaata agtgacttag agaaaattat
     1681 tgattttact ggtgttatgt tgcgggatta aattgtttga ttatattact gttgggcgga
     1741 aaaatgacgt aagttgacgt tcgacagggg taagcgaaac ggtaaaaaga taaatattaa
     1801 atgaatttag gatttttccg gcttcgttaa agaaagttaa aatgccgcca gcggaactgg
     1861 cggctgtggg attaactgcg cgtcgccgct ttcatcggtt gtacaaaagc tttcagtgcc
     1921 gccagcattt tctttggctc attaatatgt tgctcgatga ttttaacaat cgcagaacca
     1981 gaaatcgcgc ccgcagctcc tgcatcaatc gctgctttta cctgatccgg ggcggaaata
     2041 ccaaatccct gcaatggagg tgccgcgttg tactctttca gcttcgcaac cagatgattg
     2101 aggggtaacg cggcgcggtt ttctgcgccg gtcacgcccg ctcgtgacag caaatatgtg
     2161 taaccacgac cgtaagaggc tatctggcgc agcaggtcgt catcggcatt tggcgggcag
     2221 atgaagatag gtgcgacatt atgacgcaac gcggcctggc ggaagggcgc ggactcttca
     2281 actggcacat cggcaaccag caccgaatcg acgccgactt tctcgcactg ggcataaaac
     2341 tcatcaatgc ctttgttaaa caccaggttg gcatacatca gcaggccaat gggaatggtc
     2401 gggtgtttct ggcgaatgag tgccagcatc tcaaagcact gcgccggggt cacacctgcc
     2461 gcaaaggcac gcagtgtggc gttttgaatc gtcgggccat ctgccagtgg gtcggagaag
     2521 gggatgccta actccagcgt gtcagcaccg gcttcaatta gcgtatcgat aattttcagc
     2581 gactgctcaa tgcccggatc accgagcgtg acgaaaggaa cgaatgcgcc ttctttgcgc
     2641 tccttcaact gggcaaacag agattcgtag cgttccatca gatttcccct cgtgctttca
     2701 aaatatcgtg aacggtgaag atgtctttat cgccgcgacc ggaaaggtta accaccagta
     2761 gctgctcttt ttccgggttt tcgcgcatca ttttcagcgc atgggccagg gcgtgggagg
     2821 attccagcgc cgggataatc ccttcgtgca ggcacagcgt tttgaaggct tccagtgctt
     2881 catcatcggt aattgacaca taatcagcgc gtccagtgct gttaagatac gcgtgttgtg
     2941 ggccgacgga cggaaaatcg agtccggcgg agatggagta agattcttca atctgcccgt
     3001 cttcggtttg catcatcggc gctttcatac cgaaatagat acccacgcga ccatgtttta
     3061 gcggtgcgcc gtgctcgcca gtttcgatac cgtgaccacc aggctccaca ccaatcaggc
     3121 cgacgttggt ttcgttgatg aaatctgcaa acatgccgat ggcattcgaa ccgccgccaa
     3181 cacaggcgat aacggcatcc ggcaggcgac cttctctttc cagaatctgt gctttggttt
     3241 cttcgccaat catccgctga aactcacgga caatggtcgg ataaggatgt gggccagctg
     3301 cggtgcccag catatagtgc gcggtttcat aactgccgga ccagtcgcgc agcgcctcgt
     3361 tacaggcatc tttcagcgtc gcggaaccgc tatgcaccgg gatcacttcc gcacccatta
     3421 aacgcatacg aaaaacgtta ggcgactggc gttcaacgtc tttggccccc atataaatac
     3481 ggcatttcag gccgagcagg gcgctggcaa gggccgacgc cacgccatgc tgaccggccc
     3541 cggtttcggc gatgatttcg gttttaccca tccgcttcgc cagtaaagcc tgaccgagca
     3601 cctggttagt tttatgcgcg ccgccgtgca gcaaatcttc gcgcttcaga tacagcgtgg
     3661 tgttcgtgcc agccgtaatg ttctggcatt tggtcagcgc ggttggacgc ccggcatagt
     3721 ttttcagcag gtcgttgaac tgagcctgaa attcaggatc tttttgcgcg ctgacaaaag
     3781 cttcttccag ctggcgcaga gcaggcatca ggatttgtgg cacgtacatg ccgccaaact
     3841 caccaaaata ggggttaagt aatgttgtca ttgttccttt ccttaatatg cgcgcagcgt
     3901 ctggaaaacc gaggccaaaa gacgtgcgtc tttgatgccc ggttgcgact ctacagcaga
     3961 attaaaatca agtccggcgc agccggtttg tgccgcttcc acgcagttat ctgcgcctaa
     4021 gccccccgcc agcagaacgt tgccaagcga ttgaccattt aatagtgacc agtcgaaacg
     4081 ttgcccgctt ccaccctggc cgttgtctaa aacatattta tcaacgtgct gaagctcgcg
     4141 ggcgggcagg gtttcaccga cgcttaatgc tttccagatg gcaacgtgtg ctggcagagc
     4201 ttcacgcagc gtatcgatat acagctgatc ttcattacca tgcagttgca ctgccaccag
     4261 tgataacacc ttagctttgt ccagcacatc ggcaatatcg tgattgcgga acacgccaac
     4321 atactgcaac ggtgccgcag ccatcacttc ctgcgcctgt tcaacgttga cgcaacgcgg
     4381 tgatgtcgca acaaaaatca acccaccgta aatcgcgccc gcgtcgtacg ctgctttagc
     4441 atcttgccca cgcgtcaggc cacatacttt attctcaccc agcaacaccc gacgcacggc
     4501 ggcgtgcaaa tcgtcatggg ccatcaacgc cgaaccaatt agaaaaccgt cagcgaagtg
     4561 gcttaactcg cgcacctgag cgtaagtatt gatgcctgat tcgctgatta ccgtcacgtt
     4621 gtgccccagt ttcggcgcaa gctcgcgggt acggttgaga tcaatcgaca aatcacgcag
     4681 atcgcggttg ttgatgccaa cgacctttgc ccccaatgca atggcgcgct ccagttcctc
     4741 ttcattactg acttcggtca gcacacccat cttcagactg tgggcgacgg cggcaagctg
     4801 gcgatattgt tcgtcatcca gtactgaaag cattaataag caggcatcgg cctggtaata
     4861 gcgcgccaga tagatctggt aagggtcgat aatgaagtct ttacataaaa tcggctgcgg
     4921 ggcgatttgg ctgacgatgg ggaggaaatc aaagctcccc tgaaaatatt tctcatcagt
     4981 cagcaccgaa attgccgaag cgtaatgttt ataaatggcg gcaatgcgtg ctggatcgaa
     5041 atcatcacag atcacgcctt ttgacggcga cgctttcttg cactccagaa taaacgccgt
     5101 gcgcgcaccc tgtagcgcat cataaaaatg tcgcgtgctc ggctgaacct cattctgaaa
     5161 actggccagc ggttgctgct gtttgcgggc ttctacccaa atcgccttgt ctgcgacgat
     5221 tttcgctaaa acggtttgca tcatttaccc tcgtgccgcc agtgcggtga ctctgtcgta
     5281 agcggaacca ctgcgcagta cctcaagaac ggtttgcgca ttggcttgca gatcttcatg
     5341 gccatgcagg cgcattaaca tggcgacatt cgccgcgacg gctgcttcat gggcggcgtc
     5401 gcctttacct tgtaacaagc gtgttaaaat gtcacggttt tcttccggtg ttccgcctgc
     5461 cagttgctcc tggtggtagg gagtcaggcc aaaatcttca gcggtcaatt gatagctctt
     5521 aatttcgccg tcatgcagct cggcaacgat tgtcggcgcg tgtaatgaaa cttcatccat
     5581 cccgccgctg tgcaccaccg ccgcgcgttg ataccccagc acgcgtaagg tttcggcaat
     5641 cggcagcacc agttccggac tataaacacc aattaacgcc agcggcggat gcgccgggtt
     5701 aatcaatggc cccagcacat tgaacagggt gcgggttttt agttgctggc gaaccggcat
     5761 tgcatggcga aatccggtgt gatatttcgg tgcaaagagg aaacatacac ctaactcatc
     5821 cagcgcctgg cgcgatttat cggcgttcat atcaagatta ataccgaacg ccgccagcag
     5881 atccgacgag ccagatttac tggagacgct acggttgccg tgtttcgcca ctttcagccc
     5941 acaggccgcg gcgacaaacg cactggcggt ggaaatattg atgctgttgc tgccgtcacc
     6001 gccggtgccg acgatatcgg caaacagata atccggacgc gggaacggcg cggcgttttc
     6061 cagtagcgcg gttgctgctc cggcgatctc gttcgggtgc tcgccgcgaa ttttcatgct
     6121 caccagcgcc gccgccaggt gttccggttt cagttcacca cgtaccaccg ctgaaaatag
     6181 ctgatgactt tcctgctggc taagggtctg cgcctgatac agtttttcca gaatcggttg
     6241 cagcgtgttg gttggctcta gtttctgctg cgcccaggcc agcgtttgtt ccagcaggcg
     6301 agcgccctgg gtggtgagaa tggattccgg gtggaactgg aatccgcaaa cgcgatctgc
     6361 atcgtgacgc accgccatca ccatgccatt aaaatgggcg ttgatggtta aaccggccgg
     6421 aatattactg ccaaccagcg agtgataacg cgccaccggc agcgggtttg ttaatccggc
     6481 aaacatcgcc tgaccgtcat gttcaatgct cgacgcttta ccgtgaagaa tttcgcccgc
     6541 ctgaccgaca tagcccccgt aagcttcgac aatcgcctga tgaccaaggc aaatgccaat
     6601 aataggcagc ttaccgcgca ggcgggtaag cagttcaggc atacaaccag cttcgctcgg
     6661 cacaccgggt ccaggagaaa gcatcagcac cggattgctc atcgtcgcaa ggcgttcaat
     6721 taaggtctgc gccggaatat ggttgcggta aatcaccacg ttatgaccat tgctgcgcaa
     6781 ctgatctgcc aggttgtaag taaaagagtc gatattatcg agcagcagaa tgtcagccat
     6841 tagaacgtct cctgtgcatg atgcgcggtg gcaatagcgc gcagtacagc gcgggcttta
     6901 ttacgggttt cgtcggcttc cgactgcgga acagaatcaa ggactacgcc agcaccggct
     6961 tgcacggtag cgataccgtt ttccaccagc gccgagcgga tcacaatgca ggtgtcgaga
     7021 tcgccgtgcg cggtgaaata acctaccgcg ccgccgtagc tgccgcggcg acgaccttca
     7081 gcctcggcaa ttaactgcat agcgcgtact ttcggcgcac cgcttaacgt ccccatattc
     7141 atacaggcgc ggtaagcgtg cagggcgtcg agatcgtggc gcagctcacc gaccacgcgg
     7201 gagaccaggt gcatcacgta agagtaacgg tcaactttgg tgaggtcggc gacgtagcgg
     7261 ctgccggggg tgcaaatgcg tgccaggtca ttacgggcga gatccaccaa catcagatgt
     7321 tcagaaagct ctttatgatc ggtacgcatt tccagttcaa tacggctttc aaggtccctg
     7381 tcgagcgagc catcggcgcg acgaccgcgt ggacgtgttc cggcaatcgg gtagatctca
     7441 atctggcggc tggtggcgtc atacttgagc gaactttccg gcgacgcgcc aaacagggtg
     7501 aaatcattat cctgcataaa aaacatgtac gggctgggat tactcttttt cagcacgtaa
     7561 taggccgcca gcggtgacgg gcagggcaga gagaaacggc gagatggcac cacctggaaa
     7621 atttctccgg cgcgaatcgc tttttgcaac aaacgcacta cgccaccgaa ctcttcatcg
     7681 ctctggttac attcacaacg catatgcggc acggaaacca ccggcagcgg tggcgcggtt
     7741 tcggtcagtt gctggcgaag atcgttcagg cgagcagtga gacgttgttt ttcttcttca
     7801 ttcggagcaa acaggctggc ctgaatgcgg gtgctttttt tctgatggtc aatcaccatc
     7861 agagtttcag cgagataaaa acagaaatca gggcagttat tttccgctga cagttgcggt
     7921 aaatcttcaa accccgccac aaggtcataa gagaacaggc cgccgaagaa catggcttct
     7981 cgttcttcct tcggtacatt caacagattc tgcaataagc ggaaagcgtc aaaaaccgaa
     8041 agggagcata agcgagcgtc ttcatccagc agtggaccaa caggggggaa gcgcagcaca
     8101 cggcagtttg gtaattgttc attttccaca cccgcaggca gggcgttatc cagtagtgtc
     8161 agcagggctt cgccgttgcc ggaaagggac tggattgtga cagtgtcacc taaagctgta
     8221 atgcgcagcg cactgtctac cagcagcagg ctttttaaat catctttgct gtcgatatct
     8281 gcggattcca gcagcagcgt tgccggacga tccccacaca attggtgaaa aagcgcggtg
     8341 ggattgtcgc gataagcgcc ttcgcaggtt agcagttcga gagtcggttt ttgtgtttgc
     8401 attgttattc tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc tgggtatctg
     8461 attgctttac gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc aacctttcag
     8521 tacgaaaatt gctttcattg tcgataccct ttttacgtga acttgcgtac tagttaacta
     8581 gttcgatgat tgattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc
     8641 ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat tacgcagtga
     8701 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg
     8761 tgcaccgtgc agtcgagatg cgcgtcggca ccctggcgat caccgaccat gacaccacag
     8821 ctgcaatcgc gccagcaaga gaggaaattt cacgttcggg actggcgctg aatcttattc
     8881 ccggcgtgga aatttccacg gtctgggaaa atcatgaaat tcatattgtc gggctgaata
     8941 ttgatattac tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa cggcgcaatc
     9001 agcgggcgca gcaaattgcc gaaagacttg aaaaagcgca tatacctggg gcgctggaag
     9061 gcgcacaacg actggcgcag ggcggcgcag tgacgcgcgg tcattttgcg cgtttccttg
     9121 ttgagtgcgg caaagccagt tcaatggcgg atgtctttaa aaagtatctg gcgcgcggga
     9181 aaaccggata cgttccgcca cagtggtgta caatagaaca agctattgat gtcattcatc
     9241 attctggcgg taaggcggtg ctagctcatc ctgggcggta caatcttagt gctaaatggc
     9301 tgaaaagatt ggttgcgcat tttgccgaac acgacggcga cgcgatggaa gtcgcgcagt
     9361 gccagcaatc gcccaatgaa cgtacccagc tggcgacgct tgcccgccag caccatttat
     9421 gggcatcaca aggatctgat tttcatcagc catgcccgtg gatcgaactg ggtcgtaaac
     9481 tctggctgcc cgcaggcgtt gaaggcgtct ggcagctatg ggaacagccg cagaacacca
     9541 cagagaggga attatgagcc agttttttta tattcatcct gataacccgc agcaacgtct
     9601 aatcaaccag gcggtggaga tcgtgcgtaa aggcggggtg attgtttatc caactgattc
     9661 cggctatgcg ctcggctgta aaattgaaga taaaaacgcc atggagcgta tttgtcgtat
     9721 tcgccagctg ccggacggtc acaactttac cctgatgtgt cgcgatcttt ctgaactctc
     9781 gacctattca tttgtcgata acgttgcgtt tcgtttaatg aaaaacaaca cgccgggcaa
     9841 ctataccttc atcctgaagg ggacgaagga ggtgccacgc cgtctgttgc aggaaaaacg
     9901 caaaaccatc ggtatgcgtg tgccttctaa cccgattgcg caggcgttgc ttgaagcact
     9961 gggcgaaccg atgctttcca cttcgctaat gctgccaggc agcgaattta ccgaatccga
    10021 tcccgaagaa atcaaagacc gtctggaaaa acaggtcgat ttgattattc acggcggcta
    10081 tctcgggcag aaaccaacaa cggttattga tcttaccgat gatacgccgg tcgtggtgcg
    10141 tgaaggcgta ggtgatgtga agcctttctt ataacaaatt gcgttccacg gatggaagac
    10201 tatggcaggg aagtttcgct gcattttgct gttgatagtt gggctttttt tctcttcgtt
    10261 aagttatgcg aaaaacacgg agatgccttc ttatgaagaa gggatctcgc tctttgatgt
    10321 tgaagccact ctgcaaccgg atggggtgct cgacatcaaa gaaaatattc attttcaggc
    10381 gcgaaatcag cagattaagc acggatttta tcgtgattta ccacgactat ggatgcagcc
    10441 tgatggggac gctgcactgc tgaactatca tattgttggc gtcacccgtg atggtattcc
    10501 tgaaccctgg catcttgact ggcatatcgg gttaatgagt attgtcgtgg gcgataaaca
    10561 acgtttcttg cctcaaggcg actatcatta tcaaattcat tatcaggtta aaaatgcttt
    10621 cctgcgtgag ggagattcag atctgttaat ctggaacgtg actggtaacc actggccgtt
    10681 tgaaatctat aagacccgat tttcactcaa gttccctgat atcgcgggta atccatttag
    10741 cgaaatcgat ctctttactg gagaagaggg cgacacatat cgaaatggcc gcatccttga
    10801 ggacggaaga attgaatcca gcgatccgtt ttatcgtgaa gatttcacgg tactctaccg
    10861 ttggcctcac tcgttactta gcaatgcccc ggctccacaa acgacgaata ttttcagcca
    10921 tcttctttta ccctccacgt catcgttgtt aatttggttt ccgtgtctat tcctggtttg
    10981 tggatggtta tatctctgga agcgcaggcc gcaatttacg tcggtagatg ttttacggat
    11041 gcagaaattt tatctgccgc gtaaaaagtc ttcgttttac cggcctgata cttttttgca
    11101 atggggtgga ctggcaatat tggcggtcat tctttacggt aacctgagtc ctgtaggctg
    11161 ggcaggaatg agtctggtgg gcgatatgtt tattatgatc tgctggcttc ttcctttttt
    11221 attttgttcc cttgagcttt tgtttgcccg cgatgatgac aagccttgcg ttaatcgtgt
    11281 aatcatcact ttgtttttac cgctgatttg ttcaggcgtg gccttttatt ctctctatat
    11341 caatgtcgga gatgtattct tttactggta tatgccagcg ggttatttta gcgctgtttt
    11401 cctgaccggt tatctcactg gcatggggta tatttttctg ccaaagttta cccaaactgg
    11461 gcagcaacgt tatgcccacg gtgaagctat cgttaactat ctcgcgcgta aagaggcagc
    11521 aacacacagt gggcgtcggc ggaaagggga aacacggaaa ctggattacg cgttgctagg
    11581 ttgggctgtc tcagcaaacc ttggaagaga atgggtagca cgtatcaccc catcactcac
    11641 agcggctgtt cgcgccccgg aaattgcccg tagtggcgtt ttgttctcat tacagatgca
    11701 cctgagtctg ggggccaata ccagtttatt ggggcgaagt tattccggtg gtgctgcggg
    11761 tggcggaggc ggtggtggct ggtaattaag ctgatgttaa tcgaaacaag ctttaaccat
    11821 aaagatagtg tagtatgttg cgcctcaaag ccaggccagt taaaacgtcg agtcgtttac
    11881 ttaaggcctg aagagttcaa acagacttta ctatcagtga gtcagatgta agttctgact
    11941 cactgttgca caaaagcata tcagacgcct gggaaggcga caccaaaagg aagctctatg
    12001 agcgaaaagc tacagaaagt gctggcgcgt gccggccacg gttctcgccg tgaaatcgaa
    12061 tctattattg aagccggtcg tgtgagtgtt gatggcaaaa ttgctaaact cggcgatcgc
    12121 gttgaagtta cccctggatt gaaaatccgt atcgatggtc acctgatttc ggtacgtgag
    12181 tccgctgaac aaatttgtcg cgttctggcc tattataaac cggaaggtga gttgtgtacc
    12241 cgtaacgatc cggaagggcg tccaacggtg tttgaccgtc tgccaaagct gcgcggtgca
    12301 cgctggattg ccgtaggtcg tctggacgtt aacacctgtg gtctgttgct gttcaccacc
    12361 gacggtgaac tggctaaccg tttaatgcat ccaagccgtg aagttgaacg tgaatatgcc
    12421 gtgcgtgtat ttggtcaggt tgacgacgcg aaactgcgtg atttaagccg tggtgtgcag
    12481 ctggaagatg gtccggcagc ctttaaaacc atcaagttca gcggcggcga agggatcaac
    12541 cagtggtaca acgtgactct gaccgaaggc cgtaaccgtg aagttcgtcg tctgtgggaa
    12601 gcggttggcg tgcaggtaag ccgcctgatc cgtgttcgtt acggtgatat cccactgccg
    12661 aaaggtctgc cacgcggcgg ctggacggag ctggatcttg cccagactaa ctatctgcgc
    12721 gaactggtgg agttgccgcc agaaaccagc tctaaagtcg ctgtagaaaa agaccgtcgt
    12781 cgcatgaagg cgaatcagat tcgtcgtgcg gtgaaacgtc acagtcaggt gagcggcgga
    12841 cgtcgttctg gtgggcgtaa taacggttaa tcaacgtatg ccggacgtca tatccggcat
    12901 ttttacagat tagtaatcaa tccctatctg cgcttttacg ccagcatcaa acgcatgttt
    12961 cacggggcgt aactcactta ccgtatccgc cagttcgagt atatcccgat gacagccccg
    13021 acccgtgata atcaccgtct gctgatgtgg acgttcattt aacgcctgca acacttcttc
    13081 cagtggcaaa tagtcatacg ccaccatata agtcagctca tcaagcaaaa ccaaatccag
    13141 tgaggaatca gcaagcatcc gctttgcatg ttgccagact tcgcggcagg cggcggtatc
    13201 agactcgcgg ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc
    13261 atgtggctcc agcagattgc gttcgccatt aggccaggtg cctttaataa actgcacgac
    13321 gcccactttt tttccgtgac caactgcgcg tgttgccgta ccaaatgccg cggtggtttt
    13381 gccttttcca ttgccggtaa agacgatgat aataccgcgt tcatcctggg cctgggccat
    13441 acgagcatct actttttctt tcactcgctg ctgacgctgt tggtagcgtt catcactcat
    13501 tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg
    13561 ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat cagcgggtgt tttaagtttc
    13621 tgtggatctt cggtcgggaa ggcgctggca cgcattgcgg tgcgcgtacc gcctgggtta
    13681 atgcagttga cacgcaggcg ctgctgatat tcatcggcca gtacctgcat catcccttcg
    13741 gtggcaaatt tcgacgctgc atatgcaccc cagttggctc gtccctgacg tccaacgctt
    13801 gatgaagtaa agaccagtga accggcgtcc gatttgagta ataaaggaag tagtgcctgg
    13861 gtgagcataa aggtggcatt aacgttgacc tgtatgacgt cctgccagac ctgtggattt
    13921 tgttcgctca ttggacaaac atcgccgagt aatccggcat tatgcaaaac accatccaga
    13981 cgcggataat taacggcaat gcgctgtgcc agttgttggc aatcttcgga cctgcaggtc
    14041 agcaaatcga gaataaacca ctgtggctga cgcccagttt cttcgtttat gtggctggct
    14101 acctgacgta atttttcttc attacggccc aacagaatca ctgtcgcacc atagcgtgca
    14161 tacgtcatcg cggcttcacg accaatacca tcactggctc ccgtcaccag gataatgcga
    14221 tcattgagta aatcttgttt tggctggtaa tgcatggcta ctcctcaacg acgttgtctg
    14281 tcgcgctgcc agtgtacttt atgactttgg ggctttatgc ctgaaacaga agggcatttc
    14341 aatcagcctc gggcgataac accgcaaaat ggatactttg tcatactttc gctgcaataa
    14401 catctctgcg agacggctta acatgcctgt tgtaaactgt gagccaaagc gttgtttaac
    14461 caaggtgggg actcgtggaa ttgttgtctg aatatggttt gtttttggcg aaaatcgtta
    14521 ccgttgtgct agcgattgcg gcgattgccg ccattattgt caatgttgct caacgtaata
    14581 aacgccagcg tggcgagtta cgggtcaaca atctcagcga acagtataag gagatgaaag
    14641 aagaactggc cgcggcgctg atggactcac atcagcaaaa acagtggcac aaagcgcaga
    14701 agaaaaagca caagcaagaa gcgaaagcag caaaagcgaa agccaaactg ggcgaggtgt
    14761 caactgacag taaaccccgc gtctgggtgc tggactttaa aggcagcatg gacgcccatg
    14821 aagtgaactc gctacgtgaa gagataacgg cggtacttgc agcattcaaa ccgcaggatc
    14881 aggttgtgct ccgtctggaa agccctggtg gcatggtgca tggttacggg ttggcggctt
    14941 cgcagctgca gcgtctgcgt gataaaaaca ttccattaac tgttacggta gacaaagttg
    15001 ctgccagcgg tggttacatg atggcctgtg tggcggacaa aattgtttcc gcaccgtttg
    15061 ctattgtggg ttccattggg gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa
    15121 gcaaagatat tgatatcgaa ctgcacaccg ccgggcagta taagcgtacg ctgaccttgc
    15181 tgggtgaaaa taccgaagaa gggcgggaga aattccgcga agagctgaac gaaacgcatc
    15241 agttatttaa agattttgtg aagcgtatgc gtccgtctct ggatattgaa caggtggcaa
    15301 cgggtgaaca ctggtacgga caacaggcgg tagagaaggg cctggttgat gaaatcaaca
    15361 ccagtgatga agttattctt agcctgatgg aaggccgaga agtggtcaat gtacgctata
    15421 tgcagcgtaa acgactcatt gaccgattca ccggcagcgc ggcagagagc gccgatcgat
    15481 tattgctacg ctggtggcag cgtgggcaaa agccattgat gtaaaagaca aacgcgaggg
    15541 taagacctcg cgttttgctt taatcaacca gatgatattt ttctgaaagc acatgggcca
    15601 ggtgtttgaa catattaaac accgcggtgc ttttggctgt tggcaatcct tgttcatcta
    15661 aaaagtagtc gccggtaaat accagcacgc cattacgctg tgtgacgccg gtggcttcaa
    15721 tccctgcgag cgtatcctca tgctcacgaa tgattttgtt ggcctctttc aacagcgttt
    15781 cgcggtcgat gggttgtgtc tctttgttca ttatttactc cttaaacaag gacattagtc
    15841 tacgccaggc atggcttgca gacaaatata ccacgctggt ggcaagagcg ccttactggc
    15901 aactttggat tttgcatgct aataaagttg cgtatcggat tttatcaggt acagtgtgac
    15961 gctttcgtca atctggcaat agatttgctt gacattcgac caaaatttcg tcgtgctata
    16021 gcgcctgtag gccaagacct gttaactcag tcacctgaat tttcgtgaac agagtcacga
    16081 caaggggttg atatccgcag agagcgagtc catatcagta actcgttgcc agtggaaggt
    16141 ttatcaacgt gcgacgcatt cctggaagaa tcaaattagg taaggtgaat atgggtaaag
    16201 ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg
    16261 actacgtggt gaaatccagc gtcggtcaca tccgcgattt gccgaccagt ggctcagctg
    16321 ccaaaaagag tgccgactct acctccacca agacggctaa aaagcctaaa aaggatgaac
    16381 gtggcgctct cgtcaaccgt atgggggttg acccgtggca caattgggag gcgcactatg
    16441 aagtgttgcc tggtaaagag aaggtcgtct ctgaactgaa acaactggct gaaaaagccg
    16501 accacatcta tctcgcaacc gaccttgacc gcgaagggga agccattgca tggcacctgc
    16561 gggaagtgat tggtggtgat gatgcgcgct atagccgagt ggtgtttaac gaaattacta
    16621 aaaacgcgat ccgccaggca tttaacaaac cgggtgagct gaatattgat cgtgttaatg
    16681 cccagcaggc gcgtcgcttt atggaccgcg tggtggggta tatggtttcg ccgctgctat
    16741 ggaaaaagat cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggca gtccgcctgg
    16801 tggtcgagcg tgagcgtgaa attaaagcgt tcgtgccgga agagttctgg gaagtcgatg
    16861 ccagcacgac cacgccatct ggtgaagcgt tggcgttgca ggtgactcat cagaacgaca
    16921 aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag
    16981 cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt
    17041 ttattacctc tacgctgcaa caagctgcca gcacccgtct tggatttggc gtgaaaaaaa
    17101 ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg cgtaccgact
    17161 ccactaacct gagtcaggac gcggtaaata tggttcgcgg ttatatcagc gataattttg
    17221 gtaagaaata tctgccggaa agtccgaatc agtacgccag caaagaaaac tcacaggaag
    17281 cgcacgaagc gattcgtcct tctgacgtca atgtgatggc ggaatcgctg aaggatatgg
    17341 aagcagatgc gcagaaactg taccagttaa tctggcgtca gttcgttgcc tgccagatga
    17401 ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc cgcctgaaag
    17461 cacgcggtcg tattttgcgc tttgatggct ggacgaaagt gatgcctgca ctgcgtaaag
    17521 gcgatgaaga tcgtatctta cctgcagtcg ataaaggcga tgctctgacg ctcgttgaac
    17581 tgacaccagc ccagcacttt accaagccgc cagcccgttt cagtgaagca tcgctggtta
    17641 aagaactgga aaaacgtggt atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca
    17701 ttcaggatcg tggctatgtg cgagtagaaa atcgtcgttt ctatgcggaa aaaatgggcg
    17761 aaatcgtcac cgatcgcctg gaagagaatt tccgcgagtt aatgaactac gacttcaccg
    17821 cgcagatgga aaacagcctt gaccaggtgg caaatcacga agcagagtgg aaagctgtac
    17881 tggatcactt cttctcggat ttcactcagc agttagataa agctgaaaaa gatccggaag
    17941 aggggggtat gcgtccgaac cagatggttc tgaccagcat cgactgcccg acctgtggtc
    18001 gcaaaatggg gattcgcaca gcgagcaccg gggtattcct tggctgttct ggctatgcgc
    18061 tgccgccgaa agagcgttgc aaaacaacca ttaacctggt gccggaaaac gaagtgctga
    18121 acgtgctgga aggcgaagac gctgaaacca acgcgctgcg cgcaaaacgt cgttgcccca
    18181 aatgcggcac ggcgatggac agctatctca tcgatccgaa acgtaagttg catgtctgtg
    18241 gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc attaaaggtt
    18301 atgacggccc gatcgttgag tgtgaaaaat gtggttctga aatgcacctg aaaatggggc
    18361 gtttcggtaa atatatggcc tgcaccaacg aagagtgtaa aaacacgcgt aagattttac
    18421 gtaacggcga agtggctcca ccgaaagaag atccggtacc attaccggag ctgccgtgcg
    18481 aaaaatcaga tgcctatttc gtgctgcgtg acggtgctgc cggtgtgttc ctggcggcca
    18541 ataccttccc gaaatcgcgt gaaacgcgtg cgccgctggt ggaagagctg tatcgcttcc
    18601 gcgatcgtct gccggaaaaa ctgcgttatc tggccgatgc gccgcagcag gatccggaag
    18661 gtaataagac tatggttcgc tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa
    18721 aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg gttgaaggga
    18781 aaaaataacc tttaattctg tcaggttttt ataaacaaag ggtcgcgaaa gcggcccttt
    18841 tttattgcat attatttttt cttcacacct atacactaag gctataaatg atatagtggt
    18901 tatagttagc acctttttta ttattaaatc gtattagtca cccgccaggt gtgacgaaaa
    18961 aacgatgttc tgatggcgtc taagtggatg gtttaacatg aaattacaac aacttcgcta
    19021 tattgttgag gtggtcaatc ataacctgaa tgtctcatca acagcggaag gactttacac
    19081 atcacaaccc gggatcagta aacaagtcag aatgctggaa gacgagctag gcattcaaat
    19141 tttttcccga agcggcaagc acctgacgca ggtaacgcca gcaggacaag aaataattcg
    19201 tatcgctcgc gaagtcctgt cgaaagttga tgccataaaa tcggttgccg gtgagcacac
    19261 ctggccggat aaaggctcgc tgtatatcgc caccacgcat acccaggcac gctacgcgtt
    19321 accaaacgtc atcaaaggct ttattgagcg ttatccccgc gtttctttgc atatgcacca
    19381 gggttcgccg acacaaattg ctgatgccgt ctctaaaggt aatgcagatt tcgctatcgc
    19441 cacagaagcg ctgcatctgt atgaagattt agtgatgtta ccgtgctacc actggaatcg
    19501 ggctattgta gtcactccgg atcacccgct ggcaggcaaa aaagccatta ccattgaaga
    19561 actggcgcaa tatccgttgg tgacatatac cttcggcttt actggacgtt cagaactgga
    19621 tactgccttt aatcgcgcag ggttaacgcc gcgtatcgtt ttcacggcaa cggatgctga
    19681 cgtcattaaa acttacgtcc ggttagggct gggggtaggg gtcattgcca gcatggcggt
    19741 ggatccggtc gccgatccgg accttgtgcg tgttgatgct cacgatatct tcagccacag
    19801 tacaaccaaa attggttttc gccgtagtac ttttttgcgc agttatatgt atgatttcat
    19861 tcagcgtttt gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc
    19921 caatgaagaa attgaggtca tgtttaaaga tataaaactg ccggaaaaat aatttcttgc
    19981 gttattttcg gcacctttta tgtagcgaag gtgccggaat atattctctt ttgttactta
    20041 tttgtatacc gtgcacaata tttttatccc tcatatttat agggtaaatt acctgtaaag
    20101 tagtgtctaa ttacccgcct cgcaaattcc agttttcaaa tatctatttc aggtcaaaag
    20161 attgaattat tcactggaga cgattcgtaa attcgctgga tttttgacta aagtttcttt
    20221 aggatttgtc tcatcgtatg attaattaca ccaatcgttc aacgtcttgc tgataagtga
    20281 tggcgattat atgaggttag caatgccttc tggaaatcag gaaccccgcc gcgatcctga
    20341 attaaaacgt aaagcctggc tggcggtttt tcttggttct gcacttttct gggtggttgt
    20401 cgcactgctg atttggaaag tgtggggata actatggttg gtcaggagca actggagtcg
    20461 tcaccattat gccagcatag tgacaatgaa cccgaaacga aacgggaatg ttccgtcgtt
    20521 attccagacg actggcaact aacatcgcag cagcaagcct ttatagaact gtttgctgaa
    20581 gatgatcagc cgaaa
//
LOCUS       DAZFTN010000083        19915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFTN010000083 DAZFTN010000000
VERSION     DAZFTN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            286..786
                     /gene="tssB"
                     /locus_tag="ACHKV2_003416"
     CDS             286..786
                     /gene="tssB"
                     /locus_tag="ACHKV2_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6941243.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            821..1045
                     /locus_tag="ACHKV2_003417"
     CDS             821..1045
                     /locus_tag="ACHKV2_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941244.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1096..2487
                     /gene="tssC"
                     /locus_tag="ACHKV2_003418"
     CDS             1096..2487
                     /gene="tssC"
                     /locus_tag="ACHKV2_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6941245.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2578..2991
                     /gene="tssE"
                     /locus_tag="ACHKV2_003419"
     CDS             2578..2991
                     /gene="tssE"
                     /locus_tag="ACHKV2_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6941246.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2995..4845
                     /gene="tssF"
                     /locus_tag="ACHKV2_003420"
     CDS             2995..4845
                     /gene="tssF"
                     /locus_tag="ACHKV2_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6941247.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRSFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4809..5891
                     /gene="tssG"
                     /locus_tag="ACHKV2_003421"
     CDS             4809..5891
                     /gene="tssG"
                     /locus_tag="ACHKV2_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6941248.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5916..7196
                     /gene="tagH"
                     /locus_tag="ACHKV2_003422"
     CDS             5916..7196
                     /gene="tagH"
                     /locus_tag="ACHKV2_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO6941249.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPSAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7193..7717
                     /gene="tssJ"
                     /locus_tag="ACHKV2_003423"
     CDS             7193..7717
                     /gene="tssJ"
                     /locus_tag="ACHKV2_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6941250.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKQDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7720..9051
                     /gene="tssK"
                     /locus_tag="ACHKV2_003424"
     CDS             7720..9051
                     /gene="tssK"
                     /locus_tag="ACHKV2_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6941251.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9056..9817
                     /gene="icmH"
                     /locus_tag="ACHKV2_003425"
     CDS             9056..9817
                     /gene="icmH"
                     /locus_tag="ACHKV2_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO6941252.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9826..12609
                     /gene="tssH"
                     /locus_tag="ACHKV2_003426"
                     /gene_synonym="clpV"
     CDS             9826..12609
                     /gene="tssH"
                     /locus_tag="ACHKV2_003426"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6941253.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12606..13349
                     /gene="vasI"
                     /locus_tag="ACHKV2_003427"
     CDS             12606..13349
                     /gene="vasI"
                     /locus_tag="ACHKV2_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO6941254.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13354..14765
                     /gene="tssA"
                     /locus_tag="ACHKV2_003428"
                     /pseudo
     CDS             13354..14765
                     /gene="tssA"
                     /locus_tag="ACHKV2_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            14902..18336
                     /gene="tssM"
                     /locus_tag="ACHKV2_003429"
     CDS             14902..18336
                     /gene="tssM"
                     /locus_tag="ACHKV2_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO6941255.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18347..19756
                     /locus_tag="ACHKV2_003430"
     CDS             18347..19756
                     /locus_tag="ACHKV2_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO6941256.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19722..>19915
                     /gene="tssD"
                     /locus_tag="ACHKV2_003431"
     CDS             19722..>19915
                     /gene="tssD"
                     /locus_tag="ACHKV2_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6941257.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKP"
BASE COUNT         4687 a         5484 c         5499 g         4245 t
ORIGIN      
        1 tatataattc aataagatac acaaaaactc tatatttaaa ttattgccac aatacacctg
       61 acgcgaattg cacaggttca taaaatctat ttaacgcttg cagaataccc ctctttacga
      121 tcgataaaaa gtccttttct tatttatttt gaagcgtaaa atgcggggtg atgaaggagt
      181 ggtgatttta ttttcaccgc catcaagacg gcatttccga tattttttgc ggtacgaata
      241 tgccgtgttc atggcctgta cgagataaca tgacggatca cttttatgag caaaaaattt
      301 gaaggcagcg tcgcgcctcg cgaacgtatt aatatatcgt atgtcccaaa aacagatggc
      361 cagacggctg aagtggaact tccgcttaac atgctcgttg tcggcgatac cggaaatacg
      421 caggaaacat cctcactgga tgaacgtcag gcagtttccg ttaacaagca caatttcggt
      481 gcagtgatgg ctgaagcagc cattggcctc aattttaccg tccccgccac gctgaaggga
      541 agcacaacgg acgatgaaat gaatgtggcg ctgaatatca agtcactgga tgatttttca
      601 ccagatagcg tggcccggca ggttccggaa gtgaataaat tgctggaact gcgtgaagca
      661 ctgactgcac tgaaggggcc gatgggtaac cttccggcat tccgtaccca gctccaggca
      721 ttgctggaaa atgaagagtc ccgcgagcag ttgctcaaag aaatcggcca ggtgagcaat
      781 aaataaccac cactatttca ttaaacaggg ttatccaggc atgacggcac acaaaaatat
      841 ctccgggaca taccacctct ctgttgcaga cattctgcag gtggtttacc aggtatgttt
      901 cagcccgtcc gttgaaatca accaggatgg tgtggctgcg ctgattacaa cactggatcg
      961 ccgtatcagt gatctattgg atgagattat ccatttttgt gagtttcagc agagcgcgtc
     1021 gcactggcag cgcgtacttc actgacggcg aaaagccggt ttcagaaaca cagctaaaac
     1081 agaaggattt tcagtatgtc tttacaggaa gaggaactcg tttccagtca tgccgggcag
     1141 cctgagcagg aatcgtcgct gcttgatcaa atcatggcac agacccgtat tcagcccggt
     1201 tcggaaggtt atgacgttgc ccgccagggt gtgacagcct ttattgccag catcctgcaa
     1261 tccactgcat ctgccgaacc ggtcaataaa ctggccgtgg acagcatgat tgcagatatt
     1321 gatgaacgca tcagccgcca gatggatgtc atcatccatg cgcctgcctt tcagcaggtc
     1381 gagtcgttct ggcgctcact gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc
     1441 aaggtcaacg tgcttcatgt caccaagcag gaacttctgg aagactttga gtttgcgcca
     1501 gaaattattc agtccggatt ttataagcac gtttattcgt ccggtttcgg acagtttggc
     1561 ggtgaaccca tcgctgccgt gctgggcgct tatgagttca aaaataccgc gcctgacatg
     1621 aaactgctgc aatacgtcag tgccgtgggc gcaatggcgc acgcaccgtt cctgtcctcc
     1681 gtttccccgg agtttatggg gctgaactcg tggaccgaac tgcctaatat caaagatctt
     1741 tatgccatct ttgaaggtcc ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat
     1801 tcgcgctatt tagggcttac ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca
     1861 gataaccctg ttaagaactt taactattac gaagatgtca gccagaacca cgaagattat
     1921 ctgtggggta atacggcctg gatgctggca tgcaatatcg cagacagttt tgccaaatac
     1981 cgctggtgtc caaacattat cggtccgcaa agcggcggcg cagtgaaaga tctgccggtg
     2041 catctgttcg aaacgatggg gcagattcag gccaaaatcc caaccgaggt gctggtcacc
     2101 gatcgccgcg aatttgaact ggctgaagag ggttttatca cccttaccat gcgtaaagac
     2161 tctgataatg cagccttttt ctctgcaaac tcggtacaaa aaccgaagca ctttccggga
     2221 aaagatgcag aaaccaacta taagctgggt acgcagcttc cttatctctt catcatcaat
     2281 cggttagcgc actacatcaa ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg
     2341 agcgacttag aaagagagct taatacctgg atccgtcagt atgttgccga ccaggaaaac
     2401 ccgcctgcag acgtgcgcag ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat
     2461 gtagaaggcg aacccggctg gtactaggtt gcgctaagcg tgagacctca ttttaaattt
     2521 atgggggcaa attttgagct ttccctggtt ggccggttag acagggagta acacaccatg
     2581 atactggatc gggaaagaac cacaggttct ctttttgagc gtatggaagc ctcttcggcc
     2641 agaaaccggc agggaggcag tattcattcg ctcaggcaat ccattcgcca gaacctaaga
     2701 aacattctta atacccggtc cggtagttgc cggggtgcgc ctgagctggg cattgatgaa
     2761 ccggaagggg ctgaaaactt ccgcgaatcc atgagtcggg ccatcgagca atgtatagaa
     2821 cgctatgaac cccggatctc acacgctgaa gtgcaggcag tggtttcatc cgcctccagc
     2881 ccgctggata tgacgtttca tattacagcc tgggtgacat ttaacgaaac gcatgaggtg
     2941 cttgaatttg atatggcacc gaatggcagc cagcattacc gggtggactg acaaatggaa
     3001 tttgaagaac gctattttcg ggaagaactc gattacctgc gccagcttag caagctgctg
     3061 gcaacggaaa aaccccatct ggcccgcttc ctggccgaaa aagatgcgga tccggatatt
     3121 gaacgcctgc tggaaggggt ggcttttctt accggcaatc tccgccagaa aattgaggat
     3181 gaattcccag aactgacgca cgggcttatt aagatgctat ggcctaatta cctgcgtccg
     3241 gttccggcaa tgacccttat tgaatatacg ccggatatgg ataagtcttc tgtaccggtg
     3301 ttaatccccc gtaatgagca gtttacaacc aacgccgggg aaatcagagt tgatgaagtg
     3361 ctgccctctg atgctaaaaa ggaggagccg cctccctgta ccttcaccct ctgccgggat
     3421 atctggctgc tgcccgttcg cctggagcag attgaaaacc gcagtacgac ccgtaatggt
     3481 gttatcaaca tcaccttttc ggtcgcaccg ggaacggact tccgcacgct ggatctgaac
     3541 aaacttcgct tctggctcgg caatgacgac aactataccc gtgaccagct ttatttatgg
     3601 ttctgcgaat acttgcaggg tgccgacctg actgtgggtg aacagcatat tcgcctgcct
     3661 gagtttatgc taaaagctgt cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa
     3721 aacgtccaca gcggctaccg gatccttcag gagtatttct gttaccccga tgcgtttctc
     3781 ttttttgatc tttgtggttg tccggctttg cctgacggat tgcaggcgga attctttacc
     3841 ctgcaactgc gtttttcgcg ccctttgccc gtggacatcc ggctgcgccg cgattccctg
     3901 cgcctgtatt gcgcacctgc cattaattta tttatccacc atgcagaagc catcacgctg
     3961 gacaaccggc gggcagacta tccgctggtt cccagccgcc attacccaca acattacgat
     4021 gtattttccg ttaacagtgt ggtgagccag gtccaggata tgttcaggaa aaaagatctg
     4081 gggcgtcctg tttcgacgca ggccgcgcgc cagtggccag cctttgaaag tttcagccat
     4141 cagatggaat acagccggaa gcgggaggtg gtgtactggc atcaccggac caaaacatcc
     4201 ctgttccatc gcagctttga tcataccctt gcctttatac atgctgatgg cagttatccg
     4261 tcagacgaat ctctgctcag taatgaagtg gtttcggtat cgctgacctg taccaaccgt
     4321 gagcttccgt cacaaattcg ttccggcgat atcaccggca caaccggtaa aaatgcggct
     4381 gttgcttcct ttcgcaacat tacccgcccg acgcaaccac tctggccggt cattgatggc
     4441 agcctgcact ggtccctgct ctccgccatg aacctgaatt atctgtcatt actggatacg
     4501 gacgcgctga agcaggtcat cgccaacttt gatcgccacg caatccacca tccgcagacg
     4561 gcgcggctgt cacaacaaaa gctggatgcc attgagcgtc tggagacccg ccccgttgat
     4621 cgcctgttta cgggtattcc cgtccgggga ctggcctcca cgctgtatct gcacccggag
     4681 ccgtttgtct gtgaagggga aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg
     4741 ctgtacgcca gcgttaactc attccacatg ctgaccgttg tgaacacaga aagccaggag
     4801 acatggaaat ggacggaaag aatcgggcag catcctctta tctgagtccg ggaaatcccc
     4861 cggcagataa agagcagaat gatccgctgg cacaggtttt ccataatgcc tgctcttaca
     4921 atttttttgc gatggcggag ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag
     4981 aattatccct gcgcgacgat ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc
     5041 ttgccttccc gtgcagtgat atcagtgcgc tgaaacggga tacgtcaggg gcattcagga
     5101 tgaccactac ctttatgggg ttacagggga gccagtcgcc tctccccggt tattacctcg
     5161 atcacctggc ctggaaagcg gtacatgaac aaagtccggt aggtgatttt ctggatatgt
     5221 tcagccaccg cctgacccag tttgtctggc atatctggcg taagtaccgc tatcacatca
     5281 gttttcgtaa cggcggtgtg gatgcgttct cgcagcgcat gtactccctt gtcgggctgg
     5341 gccaccgcca gcttcgcgat aagctggcga ttaatcacag caaaatgctg gcgtattccg
     5401 ggatcctggc aaatccggga cgatccccgg aaattatctg tggcctggtg tcccactgct
     5461 ttgatttgtc agaagtgacg ctgcaaaact ggcaacgtcg caaggtggat attgaaccgg
     5521 atcagcaaaa cagcctgggg agctattctc ttaaaaatgg cgaaaaactg gcgggaagat
     5581 cggtactggg caactttgtt ctgggtacac gagtacccga tctcagcgga aaattccagc
     5641 tcagtattac cagcctgacc cgaaaacagt tcctctcttt tctgccgtcc ggcgaaaact
     5701 tcctgccact gacgacgttc gtgtccttca tcctgcgcga tcagcttgcc tgggacttac
     5761 atctggggct ggcgccggag caggtgggcg caatgcgcct gggcgataac aaaagtgcgc
     5821 tgctgggctg gaccagcttc ctcggcacac cggaagaacg accctcagtc accatcaggg
     5881 tacggtcgta aattatgata atcacggagc agaatatgcc ggaagagaaa ctacagacgc
     5941 tttcattgca ggtcattaac ggcagtgagc tggaaagcgg gcgggcggcg cgctgtctgt
     6001 tcacacagca gggaaatgtc ggccatgccc ccgaatgcca ctggtcggta caggatcgtc
     6061 agcagagcat tccggcccag gcttttaccg ttatcctgca cgatggcaca ttttgtctac
     6121 gcccgcagac ggcacaactg tggctgaatc aggcaaaagt cacagcaaca tcagacctga
     6181 tacagttgcg ccagggcgat gagatccaga tcggacggct gatggtgagg gttcatctga
     6241 accggggaga tattccccat tacgatgagg aaatggccac tcccgaaacc atcgttacca
     6301 atcgcgatat gctcacggat accctgctat caacggaggg tgcgccacac tatccgggaa
     6361 tgactcaccg gcaccagctt gcagacaccg tggtaaatgg tttttctgcc gatccactcc
     6421 aggcacttca gtccgaaagc ctgattacca cgggcgatcc gctttcaggc attgcggctg
     6481 tccggccatc ggcaccgctg tccgatccgg caagtaatgg ggggatcaat actccgttta
     6541 tggatctgcc gcccatttat gccagccctg gcgatcataa tgatgacatc tctgcggcag
     6601 aaatggcgca acgccacctt gcggtcaccc ccttactgcg cggtcttggc ggctcgctta
     6661 ccgtgagcaa ttccgacgat gcggatgatt ttctggagga ggccggacga acgttacagg
     6721 ccgcaataaa aggtctgctc gatttgcagc agcagcgtaa cagcctctca gacaaacatt
     6781 tgcgcccgct ggaagataac ccgctgcgcc tgaacatgga ttacgccacc gcgctcgacg
     6841 tgatgtttgc cgaaggtaaa agcccggtac atctggcggc tccctccgcc gtcagtgaaa
     6901 gcctgcgcaa tgtccgccac cacgaagaag ctaaccgggc ggcgattgtg gagtcgcttc
     6961 gtgtcctgct ggatgctttc tcaccacaaa atctgctgcg ccgctttgtg cagtaccgcc
     7021 gcagccatga actgcgccag ccgctggatg atgccggagc atggcaaatg tacagccatt
     7081 attacgaaga actggcctcc gatcgccagc aggggtttga gatgctgttt aacgaggtct
     7141 acgcccaggt ctatgaccgg gtgcttcgtg aaaaacagcg ggagccggaa gcatgaacag
     7201 gaaggcgttt ctggcctgcg tgctgatgtg cgtcatatta accggctgcg aaacggcgaa
     7261 aaaaatcagc gaggtgatca aaaacccgga tatccaggtc gggagcctga aggagcaacc
     7321 ttcggaaata accgtcaccc tgctgactga accggacacc aataccaatg cggagggtga
     7381 atcagccgcc gtggatgtgc aactggtgta cctgactgac gactcaaaat tgcaggcagc
     7441 cgactatgac cagatagcca gtacgccgct accggatgtg ctgggtaaaa actatatcga
     7501 ccaccaggat ttcaacctgt tgccggacac catcaaaacc ctgccgccga taaagcagga
     7561 tgagaaaacg cagtttattg gtgtcgtggc ctatttctcc gatgaccagg ccactgaatg
     7621 gaaacaaatc gagacggtgg aaggaaccgg ccaccattac cgcctgctgg tgcatgtacg
     7681 tcaaagcagc atcgaaatga aaaaagagga tgaataatta tggcaaccac ccgcaacaag
     7741 gtgatgtggc aggaagggat gctgatgcgc ccacaccatt tccagcaaca gcagcgttac
     7801 aacgactacc tggataacca gcgtttccgg gccatgaatg atttatcctg gggatttacc
     7861 gaactcaccc tcaacaatga actgctggcg cagggtaaga tcatgattga cagcgcgtca
     7921 ggcacactgc ctgacggcac cgtcttttct atccccgacc aggacgcact gcccgatccg
     7981 ctgcacccgc aaaattttcc ggacgaaaga agccgcaata tttacctcgc tctgccggtc
     8041 gccagtgatg tgagaaatga aatcagcgac gggcggcgaa tcgggcgtta ccggctgaat
     8101 tatgccgatg tccgggatct gcattcagaa gaaggcgacg cgcgaacact gacgctggga
     8161 caactgacgc cgcgcattat gagcggtgca gaagatatga gtgcctatat tacgctcccg
     8221 ctttgccgta tcagcgatcg ccatgccgac ggctccctga cgctggatga cgattttatc
     8281 ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag
     8341 ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc
     8401 ggcatcgcgg atgtggcgga atttatgatg ttgcagttgc ttaaccgtaa ccagacccgg
     8461 tttacccatc gcgcccgccg atcccagctc cacccggaag atttctacct tgatcttgcc
     8521 gagttgctgg gtgaactgat gacctttaca gagccgtcac gcctgccctg cccgcttgat
     8581 gtgtatgatc atcatgacct gaccaaaaca tttaaaacac tgttaccgga agtcaaacgg
     8641 gcgctgcata ccgtactgtc gccaagagcg gtcaatctgc cgctgcatct gcgtgacggc
     8701 atctggcagg ccgatgttca tgactcggaa ctgctacagt ctgccacctt tgtactggcc
     8761 gtggcagcaa atatgcctgt cgatcagatc cagcgtcagt ttatccagca gtcgaaaatt
     8821 tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tcccgggtat tcctctgcgc
     8881 gcactgatgg tggccccccg ccagcttccc taccattccg ggttcagcta tttcgaactc
     8941 gacaagagcg gccaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgta
     9001 tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga
     9061 tgaggggtca ctgtctctgc caccatttac cggatacgac gaaaaaagcc tgcgcgacta
     9121 ccatctcgcg cttcatggca acagcctgaa tccgatgatc gacgcggcga cgccgctact
     9181 cggtatggta atgcgccttt ccaccatgaa cagccagacg atgccggagc atctttttgc
     9241 tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggaacagg gctacgaacc
     9301 gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg
     9361 taacggctgg tcgaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa
     9421 cgaagcctgg ggtggcgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa
     9481 acgttaccag gatctgctgg aatttctctg gctctgtttt tctctgggat ttcgcgggcg
     9541 ctacaaagtg gcagtacagg atcaggggga atttgagcag atctaccgcc gtctgtacca
     9601 cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac
     9661 ccaaggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt tttgtggtgg
     9721 cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca
     9781 ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat ccagattgat
     9841 cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc
     9901 tcagaatgta tgagccagca ggcagcggaa attaccgtca gccatgtgct tattcagatg
     9961 ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat tggcatggaa
    10021 gagttgcgcc aggcgttgac ggtagagaac tacacaaccg cccgttctgc ggacagctac
    10081 ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctggctgct ggcgtcggct
    10141 gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccttgct gcattcgccg
    10201 ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg
    10261 caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac
    10321 ggtaaaggcg caggcacact gacggacgcc tctgacaccc tgcttgcccg ctacgccaaa
    10381 aacatgacag aagacgcccg taacggcagg ctagccccag tactgtgccg cgaccacgaa
    10441 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccggt ggtggtgggc
    10501 gaagcaggcg tgggtaaaag cgcgctgatt gaagggctgg cgctgcgcat cgtggcaggc
    10561 caggtgccgg acaaactgaa aaacaccgat atcatgaccc ttgacctggg cgcattgcag
    10621 gccggggcat cggtgaaggg tgagtttgaa aaacgcttca aggggctgat ggcggaggtc
    10681 atttcctctc cggtgccggt cattctgttt atcgacgaag cgcataccct gattggcgcg
    10741 ggcaaccagc agggcgggct ggatatctcc aacctgctga aaccggcgct ggcgcgtggc
    10801 gaactgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgagaaagac
    10861 gccgccctgt cgcgccgctt ccagttagtg aaggtcagcg aacccaacgc tgccgaagcc
    10921 accattattc tgcgtggtct gtcggcggtc tatgaacggt ctcacggcgt gctgattgac
    10981 gatgatgcct tgcaggccgc agcaacgctg agcgaacgtt atctctccgg gcgtcagtta
    11041 ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtcg
    11101 tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga ggcggaaatt
    11161 cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag
    11221 gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc ggcttggcac
    11281 cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact gggcgtggca
    11341 gaggacgatg cggcgccgtt gccggacgca gatactgtgg aggatacgca gccagagtca
    11401 gagtcagagt cagagtcaga gtcagaacag gataataccg gtgccgtacc ggctgatgag
    11461 accgacagag aacagccgga agagaccgct gaaacagttt ccccggtaca gcggctggcc
    11521 cagctcactg ccgaactgga cgccctgcat aacgaccggt tgctggtctc cccgcacgtc
    11581 gataaaaaac agattgcggc ggtgattgcc gaatggaccg gcgtgccgct caaccgcctg
    11641 tcgcagaatg aaatgtcggt catcaccgac ctgccgaaat ggctgggcga caccatcaaa
    11701 ggccaggacc tggcgattgc cagcctgcat aagcatctac tgaccgcacg cgccgacctg
    11761 cgtcgtccgg gacgcccgct cggtgcgttc ctgctcgctg gccccagcgg cgtgggtaaa
    11821 accgaaaccg tcctgcaact ggcagagctg ctctacggcg gtcgccagta cctgaccacc
    11881 atcaatatgt ccgagttcca ggagaaacac accgtttcgc ggctgattgg ctcccctccg
    11941 ggctatgtcg gctatggcga aggcggcgtg ctgactgaag ccatccgcca gaaaccctac
    12001 tcggtggtgc tgctcgatga agtggaaaaa gcgcacccgg atgtcctcaa cctgttctac
    12061 caggcgtttg acaagggcga gatggccgac ggcgaaggcc gcctgattga ctgtaaaaac
    12121 atcgtcttct tcctcacctc caacctcggt taccaggtga ttgtcgaaca cgcggatgac
    12181 ccggaaacca tgcaggaagc cctctatccg gtgctggcgg acttctttaa acctgccctg
    12241 ctggcgcgaa tggaggtggt gccgtacctg ccgctgtcga aagagacgct cgccaccatt
    12301 atcgccggga aactggcccg tctggataac gtgctgcgca gtcgctttgg tgcagaagtg
    12361 gtcattgaac cggaagtgac ggacgaaatc atgagccgcg tcacccgcgc ggaaaacggc
    12421 gcgaggatgc tggaatctgt catcgatggc gacatgctac cgccgctctc gctgctgctg
    12481 ttgcagaaaa tggcggctaa cacggcgatt gcccggattc ggttgtcggc agtggacggc
    12541 gcatttacgg cagacgtgga agatgctcag aacgacgagt ccgtcacaaa ggatgaaacg
    12601 gttttatgag caggttgcgc gttttccggc agacaggatg gtttatcgcg ggcctgatga
    12661 cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc aatggccggt gtggcggtcg
    12721 ccgtcgccac cacgaccccg ccggatgcga cagccacgtt acaggccatg cagtcctgtc
    12781 ggcgggagtc cgcggcgctg gagcggcttg actgctatga ccacctgctg gcaccgctgt
    12841 ccccgtcagg gtttgacggg gcgctggtca aagccggttt tgtgggcgag gcctggaccc
    12901 gcgccacaga gcaggaaaaa cgccgcgagg gcaacaccac agaactgctg gtgacgcagg
    12961 tgccggggga gcgcccgacg gtggtgataa ccaccccggc tatcggccat gttccgccgc
    13021 gcccggtgct gatgttcagt tgcgtggaca atatcacccg gatgcaggtg gcgctgatgc
    13081 accctctgga tgtccatgat atcgccgtca ctctgaatgc cgacagccgg gcattgcgca
    13141 gccactggtt tgtgcgcgag aacggcaccc tgctggagtc cagccggggg ctgtccggga
    13201 ttgatgaaat taagcaactg ttcggcgcaa aaacgctgac agtggatacc ggcgcagata
    13261 acgccgcagg aaagctgacc tttaacattg acgggctggc aagggcgatt gcccccctgc
    13321 gcgatgcctg ccactgggcg ggagaataaa aatatggcaa tggacttacg cgatccgaat
    13381 gtctggatat cgcacctgct ggaaaacctg ccggaagaaa aactggcatc ggcgctgaaa
    13441 gatgacaacc cggactggga gtatatcgac ggcgaaatcg tcaagctggg gtctctggcc
    13501 cacgctcagc ttgatattcc cgaactacaa cgcagggggc tacagcttct ggcttctgaa
    13561 agcaaagact tcaggctact ggcacacctg ctcagaaccc tgcaacatgc cggtgatcca
    13621 ctgctggcac tgcacctgct aacgctatac gtggaacatt actggactgt ggccgcgccg
    13681 cagaatatgg cgcataaaaa acgctttgcc agccagatca ttaaacgttt tgaaacggta
    13741 ttgaaggctt ttcacaaaac gctgccacaa cgcagcgcga tactctgctg ggtgagctgg
    13801 cgaaactggc gcagtgctgg cagtcacata acgtcccgga actggcacag gctaccgatg
    13861 atctttttgc cctgtaccag cgtacgttta atcgtgcggc tcctgctccg gtccccactc
    13921 cggcggcctc cggtagttca ccacaaacca ccgtcacgtc tgaaagcggc gtgacgcaac
    13981 ccagtgctcc ggctccccaa atcgccatcg acagtcacga cgacaaagcc tggcgcgaca
    14041 cgctgttaaa agtggcggct attttatgtg aacgccagcc ggactcgccg cagggctatc
    14101 gcctgcgccg ccatgccctg tggcaatcca tcaccagtac accccaggcg gaaagcgatg
    14161 gacgtacccc actggctgcg gtctctgccg atatggtggc ggattaccag tcccggcttg
    14221 ccagcgcgga tatggcgctg tggcaacagg ttgagaaaag cgtattgctg gctccttact
    14281 ggctggacgg tcactgtctt tctgcacaga cggcactgcg tctgggttac aaacaggtgg
    14341 cagacaccat ccgcgatgag gtcatccgct tccttgagcg tctgccccag cttaccgggc
    14401 tgctgtttaa tgaccgcaca ccgtttctca gtgagcagac gaaacaatgg ctggctgctt
    14461 cgcccgacgg caaagttgca ccggttgcgc aaatcggtga ggaatcgcag gcagccagag
    14521 cctgttttgc tgggcagggt ctggaggcgg cgctgcgata tctggacatg ctacccgaag
    14581 gcgatccccg cgatcagttt caccgccagt accttgccgc acagttgacg gaggaggcgg
    14641 ggctgataca gcttgcgcag caacagtacc ggatgttgtt gatgataggg agtcagatga
    14701 tggtgtctga ctgggagcca tcattactta cgcagcttga acaaaaattc acggcagaac
    14761 aataaaagag aacaggagtt tttgtgttca gattaccaac accccgacta ctcagcggac
    14821 tcaaatcagc cctgcgaccg gcgatgcccc ggtttaaagt ctccgctttc tggctgctga
    14881 tactggcgtg gatttttctg cttgctgata ctggcgtgga tttttctgct ggtatggatc
    14941 tggtggaaag gcccgacgtg gacgctgtat gaagaacagt ggctcaaacc actggcgaac
    15001 cgctggctgg caacggcggc gtgggggatt attgccctgg tgtggcttac cgtccgggta
    15061 atgaagcgcc tgcaactgct tgaaaaacag caaaaacagc agcgcgaaga ggccattgat
    15121 ccgctcagtg tggagctgaa cgcccagcag cgttaccttg accgctggct gctgcgcctg
    15181 caacgccatc tcgacaaccg ccgtttcctg tggcagttac cgtggtatat ggtcatcggc
    15241 ccggcgggca gtggcaaaac gacactgctg cgcgaggggt ttccgtcaga cattatttat
    15301 gccccggagg gcgcgcgcgg cacagaacaa cgcctgtacc ttacgcccca tgtcggtaaa
    15361 caggcggtga tctttgatat cgacggcaca ctctgcgctc ccgctgatgc ggatatcctg
    15421 catcgccgtc tgtgggaaca tgccctcggc tggctgaaag aaaagcgcgc gcgccagccg
    15481 ctgaacggga ttattctgac actcgattta cccgatctgc tgaccgcaga caaacgccac
    15541 cgcgagcatc tgttacagac gctgcgcagc cgcttacagg atatacgcca gcatcttcac
    15601 tgccagttac cggtttacgt ggtactgacc cggcttgatt tattacaggg ttttgccgcc
    15661 ctgttccagt ccctgaacag acaggatcgc gatgcgattc tgggcgtcac gttcacccgc
    15721 cgtgcccatg aaaatgatga ctggcgaaca gagttgaatg ctttctggca gacatgggtg
    15781 gatcgaatga atctggcgtt gccggatctg atggtcaccc agactcacac ccgcgcgtct
    15841 ttattcagtt tttcccgcca gatgcaggga agccgtgaac cgctggtgtc actgcttgag
    15901 ggtctgcttg acggtgaaaa tatgaacgtg atgctgcgtg gtgtctatct cacctcttcg
    15961 cttcagcgcg ggcagatgga tgatatattc acccagtctg ccgcccgcca gtaccgactg
    16021 ggcaataacc cactggcgtc ctggcctctg gtggacaccg cgccttattt cacccgcagc
    16081 ttgttcccgc aggcattact cgcagagcct aatctggcaa cggagagccg cgcctggctg
    16141 atgcgttccc gtcgccgcct gacggttttc tctgccaccg gcggcgtggc ggcactgctg
    16201 ctcatcaccg gctggcatca ctattacaac ggtaactatc agtccggcat caccgtgctt
    16261 aagcaggcca aagcctttat ggatgtgccg cctccacagg gggaagatga ctacggcaac
    16321 ctgcaactgc cgctgctgaa cccggtacgc gatgccacac tggcctatgg cgactggggc
    16381 gaccgcagcc gtctggccga tatgggactg taccagggac gacgtatcgg accttatgtg
    16441 gaacagacct atctgcaact gctggagcaa cgttacctgc cctcgctgtt taacgggctg
    16501 gttaaagcga tgaatgccgc gccgccggag agtgaagaaa aactcgcggt gctgcgcgtg
    16561 atgcgaatgc tggaggacaa aagcggacgt aacaatgagg tggtgaagca gtatatggca
    16621 aaacgctgga gcgaaaagtt ccacggccag cgcgatatcc aggcacaact gatgtcccat
    16681 cttgactacg cgctggctca tactgactgg cacgcagagc gtcaggcggg cgacggtgac
    16741 gccatcagcc gctggacgcc atatgacaag cccgtggtat cagcacagaa agaactgagc
    16801 aaactgcctg tctaccagcg ggtttaccag agcctgaaaa cgcgggcgct gggcgttctt
    16861 cctgccgacc tcaatctgcg tgaccaggta gggccaacct ttgaccaggt gtttacatct
    16921 gccgatgaca acaaactggt tgttccacag tttcttaccc gttacggcct gcaaagctat
    16981 tttgtaaaac agcgcgatga actggttgaa ctgacggcga tggattcctg ggtacttaac
    17041 cttacccgca gcgtgaaata cagtgacgcc gaccgcgcgg aaatccagcg ccagctcacc
    17101 gagcagtata tcagcgacta caccgccacc tggcgggccg ggatggacaa tctgaatatc
    17161 cgcaattttg agtccatcgg acaactgacc ggggcgctgg agcaggttat cagcggcgac
    17221 cagcctttgc agcgggcgct gaccgtgctg cgtgacaaca cacagccagg cgtcttttct
    17281 gaaaaactct ctgccaaaga acgggatgag gccctggcag agccggacta ccagttactc
    17341 acccgtctcg ggcatgagtt cgccccggaa aacattaccc tggcagtaca gaaagacaaa
    17401 gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt tgcaccgcta cctgctggca
    17461 atccagaacg cgcctgtacc agggaaatcg gcgctgaaag ccgtgcagtt acggcttgat
    17521 cagaacagca gcgatccgat attcgccacc cgccagatgg caaaaacgct gcctgctccg
    17581 ctcaaccgct gggttggcag actgactgac caggcctggc atgtggtgat ggtggaggct
    17641 gtacattata tggaagtgga ctggcgcgac agcgtggtga aaccgtttaa cgagcaactg
    17701 gcaaataact atccgtttaa tccgcgttct gcacaggatg cctcactgga tgccttcgaa
    17761 cgcttcttta aaccggatgg catactggat accttctacc agcagaacct gaagctgttt
    17821 atcgataatg acctgagtct ggaggatggc gataacaacg tcattattcg cgaagatatt
    17881 attgcgcaac tggaaactgc gcagaaaatc cgtgacatct tcttcagcaa acagaacggt
    17941 ctgggaacat cctttgccgt ggaaacggta tcgctttcag gcaataaacg ccgcagtgta
    18001 ctgaaccttg acggtcagtt agtcgattac agccagggcc gtaactatac cgcccatctg
    18061 gtctggccta acaacatgcg cgaaggcaac gaaagtaagc tgacgctcat cggcaccagc
    18121 ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg cgcagttccg cctgttcggg
    18181 gccggacaac tgaccggagt acaggatggc aactttaccg tgcgctttag cgtggacggt
    18241 ggcgcgatga cctaccgtgt gcataccgac acggaagata acccgttcag cggtgggttg
    18301 ttcagccagt ttggtctgtc agacacactg tactgaggag gcagggatga atagtaacgt
    18361 actgacacaa actatcgtta ccggcagtga cccgcgcggg ctgccggaat tcagcgccat
    18421 ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct gagctgaact ggaaactggt
    18481 ggagtcgctg gcgctggcga tttttaaagc caacggtgtg gatttacaca ccgccaccta
    18541 ctatacgctt gcccggacac ggacacaggg actggcggga ttctgcgaag gtgcggaact
    18601 gctggcggca atggtaagcc acgactggga taagttctgg ccgcagggcg gcccggcgcg
    18661 tactgaaatg ctggactggt ttaactcccg aaccggcaat attctgcgtc agcaaatctc
    18721 ctttgcggaa tccgacctac cactgatcta ccgcacagag cgggcattgc agcttatctg
    18781 cgataagctc cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc tgctctattt
    18841 tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag agtaacactg agaacgccgc
    18901 acagaccacg gtcagaacgc tgatttatgc cccggaaaca caggcatctt ccacaccaga
    18961 agcggtagtg cctcccctgc ccggcctgcc tgagatgaaa gtggaagtgc gcagtctgac
    19021 agagaatccc ccacaggcca gtgtgataaa gcaaggcagt acggtaagag ggtttatcgc
    19081 agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg tggtggcagg tctatccggt
    19141 gcagcagcaa ctgttacagg ttaacgacac cgctcagggc gcagcaacgg tgtggatggc
    19201 ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa cttcttgata cctccccggt
    19261 acagccgctg gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct ggccggaaag
    19321 cctgcaacag caacaggcct cgacacaatg gaatgaggca ctcaaaaccc gcgcacagag
    19381 tagcccgcag ttgcgtggct ggttgcagac ccgccaggac ttacatgctt ttgcagatct
    19441 agtgatgcag cgcgagaaag agggactaac cctttcctat atcaaaaatg tcatctggca
    19501 ggcggagcgg ggactggggc aggaaacacc cgttgagtct ctgttgacgc agtaccagga
    19561 tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa caaattaatg agcgactcga
    19621 aggcgtgtta agccgctggc tgctgctgaa gaataatacg ataccgacga taaaaaaagc
    19681 attgaatttc aataacattc atgaatacaa aggagtttta aatggcgaat ttaatttatt
    19741 taacactaaa tggtgataat caagggctca tttcaagtgg ttgttcatca caaccatcca
    19801 ttggaaataa agctcaaaca gcacacattg atcaaattat gatttatgaa ttcatgcatg
    19861 gattaaatcg agatcaaaac gtcaaccatc atcatttgac aataaaaaaa cctat
//
LOCUS       DAZFTN010000084        19556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFTN010000084 DAZFTN010000000
VERSION     DAZFTN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..192
                     /locus_tag="ACHKV2_003432"
                     /pseudo
     CDS             1..192
                     /locus_tag="ACHKV2_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            242..1192
                     /gene="yahI"
                     /locus_tag="ACHKV2_003433"
     CDS             242..1192
                     /gene="yahI"
                     /locus_tag="ACHKV2_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO6941258.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1202..2584
                     /gene="yahJ"
                     /locus_tag="ACHKV2_003434"
     CDS             1202..2584
                     /gene="yahJ"
                     /locus_tag="ACHKV2_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6941259.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2883..3293
                     /locus_tag="ACHKV2_003435"
     CDS             2883..3293
                     /locus_tag="ACHKV2_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO6941260.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3547..4530
                     /locus_tag="ACHKV2_003436"
     CDS             3547..4530
                     /locus_tag="ACHKV2_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6941261.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4579..6063
                     /locus_tag="ACHKV2_003437"
                     /pseudo
     CDS             4579..6063
                     /locus_tag="ACHKV2_003437"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            6056..7027
                     /locus_tag="ACHKV2_003438"
     CDS             6056..7027
                     /locus_tag="ACHKV2_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6941262.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            7024..7980
                     /locus_tag="ACHKV2_003439"
     CDS             7024..7980
                     /locus_tag="ACHKV2_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6941263.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8067..9116
                     /gene="yahK"
                     /locus_tag="ACHKV2_003440"
     CDS             8067..9116
                     /gene="yahK"
                     /locus_tag="ACHKV2_003440"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO6941264.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9359..10174
                     /gene="yahL"
                     /locus_tag="ACHKV2_003441"
     CDS             9359..10174
                     /gene="yahL"
                     /locus_tag="ACHKV2_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO6941265.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10626..10835
                     /locus_tag="ACHKV2_003442"
     CDS             10626..10835
                     /locus_tag="ACHKV2_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941266.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10852..11523)
                     /gene="yahN"
                     /locus_tag="ACHKV2_003443"
     CDS             complement(10852..11523)
                     /gene="yahN"
                     /locus_tag="ACHKV2_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO6941267.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11670..11945
                     /gene="yahO"
                     /locus_tag="ACHKV2_003444"
     CDS             11670..11945
                     /gene="yahO"
                     /locus_tag="ACHKV2_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO6941268.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(12043..13629)
                     /gene="prpR"
                     /locus_tag="ACHKV2_003445"
     CDS             complement(12043..13629)
                     /gene="prpR"
                     /locus_tag="ACHKV2_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO6941269.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            13868..14758
                     /gene="prpB"
                     /locus_tag="ACHKV2_003446"
     CDS             13868..14758
                     /gene="prpB"
                     /locus_tag="ACHKV2_003446"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO6941270.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRICAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            14914..16083
                     /gene="prpC"
                     /locus_tag="ACHKV2_003447"
     CDS             14914..16083
                     /gene="prpC"
                     /locus_tag="ACHKV2_003447"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO6941271.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            16117..17568
                     /gene="prpD"
                     /locus_tag="ACHKV2_003448"
     CDS             16117..17568
                     /gene="prpD"
                     /locus_tag="ACHKV2_003448"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO6941272.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            17608..19494
                     /gene="prpE"
                     /locus_tag="ACHKV2_003449"
     CDS             17608..19494
                     /gene="prpE"
                     /locus_tag="ACHKV2_003449"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO6941273.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         4744 a         4781 c         5299 g         4732 t
ORIGIN      
        1 tcaggcattt gtcaccattg ccggatgcgg cgtgaacgcc ttatccggcc tacgaatggc
       61 acgagaattg taggcctgat aagcgtagcg catcaggcat ttgtcaccat tgccggatgc
      121 ggcgtgaacg ccttatccgg cctacgaatg gcacgagaat ttgtaggcct gataagcgta
      181 gcgcatcagg cagtctggcg ttggtcataa ccccatcacc ctctgtagcg gacataacaa
      241 catgaaagaa cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag
      301 ccagtcgatt gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat
      361 gctggcttcc gattacgaca ttgtgctgac ccacggtaac gggccgcagg tcgggctgga
      421 tttacgccgt gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa
      481 ctgtgtggca gatacgcagg gcggcattgg ctacctgatc caacaggcgc tgaacaaccg
      541 gctggcgcgt cacggcgaga aaaaagccgt caccgtggtg actcaggtgg aagtggataa
      601 aaacgatcca ggttttgccc atcccaccaa gcccattggt gcattcttca gtgaaagcca
      661 gcgtgacgaa ttacaaaagg caaaccctga ctggcgtttt gttgaagatg ctggacgggg
      721 ctatcgccgc gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ccgccattaa
      781 ggcgctgatc caacaaggct ttgtggtgat tggcgcgggc ggcggcggaa ttcctgtggt
      841 gcgtactgac gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctctac
      901 cgcgctgctg gcccgtgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa
      961 agtctgtatc cactttggta aacccgagaa gcaggcgctg gatcgggtgg atattgccac
     1021 catgactcgc tatatgcagg aaggacattt cccgcccggc agcatgttgc caaaaatcat
     1081 cgccagcctg acgttcctgg aacagggcgg taaagaagtg attatcacca cgccggaatg
     1141 cctgccagcg gcgctgcgcg gtgaaacggg cacccacatt attaaaacgt aaggacgtaa
     1201 gatgaaagaa aataatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc
     1261 cgccgcgctg tttggtacct ctgtgccgct cgcccatgcg gcggtctctg gcaccacaaa
     1321 ctgcgaagcg aacaacacca tgaaaatcac tgacccgcat tactatctcg acaacgtgct
     1381 gctggaaacc ggttttgact acgaaaatgg cgtggcggtg cagacccgca cggcgcgcca
     1441 gaccgtggag attcagaacg gcaaaattgt cgcgctgcgc gagaataagc agcacccgga
     1501 tgccacgctg ccgcactatg acgctggcga taagctgatg ctgcccacca cccgcgacat
     1561 gcatattcat ctcgacaaaa cgttttacgg cgggccgtgg cgctcgctca atcgcccggc
     1621 aggcaccacc atccaggaca tgatcaaact cgagcagaaa atgctgccgg agctgcaacc
     1681 gtacactcag gagcgggcag aaaaactgat tgatttattg cagtcgaaag gcaccaccat
     1741 tgcccgcagc cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca
     1801 ggcggtgctg gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct tcccgcagca
     1861 cggtttgctg ctgtcgaaat cggaaccctt aatgcgtgaa gcaatgcagg cgggtgcgca
     1921 ttacgtcggc gggctggacc cgaccagtgt cgatggcgcg atggaaaaat ccctcgacac
     1981 catgttccag attgcgctgg actacgacaa aggcgtcgat attcacctgc acgaaaccac
     2041 tccgtcgggc gtggcagcca tcaattatat ggttgaaacg gtagagaaaa cgcctcaact
     2101 gaaaggtaag ctgaccatca gccacgcctt tgcgctggct acgctcaacg agcaacaggt
     2161 agatgaactg gcgcatcgca tggcggcgca gcaaatttct atcgcctcga cggtgccgat
     2221 tggcacgctg catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag tgatgactgg
     2281 cactgacagc gttatcgacc actggtcgcc ttatggtctg ggcgacatgc tggaaaaagc
     2341 caatctctac gcgcagctct atattcgtcc taacgaacag aacctctccc gttcgctgtt
     2401 tctagccact ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat ggccaaaagc
     2461 gcaggatgac gccagctttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg
     2521 tatctcgccg agaaccgcaa cgttccataa agggcaactg gtgtggggga gtgtggcagg
     2581 ttgatgcgca tttgaaacgc ctgcaacggt gaatagtaag agatttaagc cccagggagt
     2641 ggggcgggaa tggtttgcta atgaatgatc ctgaacttta taatctcccg gcataatttc
     2701 caggggatta tttgagagaa atacacaaaa gtgagtttta acaaaggtat ttatcaggcc
     2761 ataaatgtga cctgcttcgg ataaaaaaga ttcagtatcg aaaaaccaat gatactcttg
     2821 ctgatggtta ccaacagtaa ttaagatgtg atttgttttt ccgtaatcgc aaaaatattg
     2881 ccatgaccat aaaagaaaaa ttctcacaaa aatatcccca tgcttccttt tgtacttttg
     2941 gcgattcagc cgcgttggcc gatcacctcg caacgttgat tgcgactggt gttaaaacag
     3001 cttcctgtgg ttcgctggcg ggatgtattg aagacaacgc atttccgatg attggtgagt
     3061 ataagattgt agaaaatagc cgtggtgagc ctgtatgtgt tatcagagtg attggcctgc
     3121 atttactgcg cttttctgat gtcaccgcgg agcttgcccg taaggaaggt gaaggtgacc
     3181 tcagtcttga atattggcgt aatgaacacc gtcgtttttt ccaggcagaa ggaagctatt
     3241 caccagaaat ggacgttatc tttgaagaat atgcccttat tgacgttgtg tagataacta
     3301 tttatctaca tgaaataagc tcacaaacat aaaatatcgc taattttgtt tattttgctg
     3361 ttttttacgt gatgaatgtc acacttatta ttgctgcccg ttttgtccat atttataacc
     3421 atatttttaa tagctgcctg aaatagagct gtttattctg cataaaaata ttcgaaaatt
     3481 cttattctct ccagcgcgct aatttgggcg aacacttcct gactaccctg caatgaggct
     3541 gaaatgatga ataaacgttt tgttatcaat atggtgtctt cgctgttatt aggcgctgcg
     3601 ctaatttctg cgccgctcca ggcagcggaa aaagtcgtgg tgaatatttc taaagtcgat
     3661 ggtatgccgt ggtttaatcg catgggcgaa ggtgtggttg aggcgggtaa agcctttggt
     3721 gttaatgcct cacaggtggg gccttcaagt actgatgctc cgcaacaagt taaaattatt
     3781 gaggatttaa tcgcccgtaa ggttaatgca attactatcg tgccaaacga tgccaacgtg
     3841 ctggaaccgg tattcaaaaa agcgcgagat gccggaattg tggttctgac caacgaatcc
     3901 cccggtcagc caagtgctaa ctgggatatc gaaatcattg ataacgaaaa atttgccgct
     3961 gaatatgttg agcatatggc gaaacgcatg ggtggcaagg gcggctatgt gatttacgtg
     4021 ggtagtttga ccgtgccgca gcataactta tgggctgact tgctggtgaa ataccagaaa
     4081 gagcattacc cggatatgca tgaagttacc cgccgcatgc cggttgccga gagtgtggat
     4141 gtctcacgtc gtactacact ggacctgatg aaaacctatc ctgacttgaa agcggttgtc
     4201 tcctttggtt caaacggtcc gattggcgcg ggacgtgcgg tgaaagagaa acgggcgaaa
     4261 aataaagtcg cagtctatgg catgatgatc ccttcacagg cggcatcact gattaaaagt
     4321 ggtgatatca ccgaagggat tacttacgat ccggcctctg caggttacgc actcgcagcc
     4381 gtcgccagca cgttgctgaa gggtgaagag atcaagcctg gattagaaat gcagaatctg
     4441 ggtaaggccg acgtagatat ggataagcga attatccgtt tccataaggt attactggtc
     4501 aacaaagaca atatcgactc actttattaa tccatttctt cggggcgaaa gccccgattc
     4561 ttattgccag gggatgttat ggaaaccttc ctttcccttc gtcatatcaa caaaacgttt
     4621 catgccacgc gagcgctgcg cgatgtttct ctcgacttta tgtcgggtga agtgcactgc
     4681 cttgccgggc aaaacggctg tggtaaatcg acgttgatta aaattatgtc cggtgtttat
     4741 cgcccggatg agggagcgga aattacgctt ggtgggaaaa actggtcaaa gctgacaccc
     4801 gccgcttcgg tggcgcaggg gattcaggtg atttattagg acctctcttt atttcctaac
     4861 ctgagtgtct gggagaatat cgccgtgaat cactatcacc acggtctgtt tgttaaccgc
     4921 cgtcgtctgc gtgaggtggc gcaggcggca atgactagta ttaacgtcac attgccactg
     4981 gatacgctcg tttctgagct ttcaattgcc cgctgtcagc tggtggcaat ttgccgtgca
     5041 ctggcgcagg acgcacggtt gatcgtgatg gatgaaccaa ccgcttcgct gacccatcag
     5101 gaagtgcaag ggttattaca ggtggtgcat caattgcgtg aacgcgggat ctgcgtggtc
     5161 tttgtcagtc atcgtctgga agaagtgatg gaagtttccg accgtatttc agtgctgaaa
     5221 gatggtgagc tggtcgggac ttttccggct gcagagatga ccacaaaaca actcggtttc
     5281 ctgatgaccg gccaggagtt tgaatatcag gtgcgagagt tgtggcaggg aaaatccagc
     5341 accccggtgc tggaggtgcg taatttaagt cgtcatgggg aatatctaaa tattaacttg
     5401 cgggtggaag cgggcgaagt ggtatcgatt gttggcctgc ttggtgcggg gcgcacggaa
     5461 ttatgtctga gtctgtttgg tatgacccga ccagatgctg gcgagatcct catcaacggc
     5521 cagctggtta cactgcatag taaccaggac gccatccgcc acggtattgg ttatgtttcg
     5581 gaagatcgca tgtcgcgcgg tttggtgatg gcgcaatcta ttgaagacaa catcattagc
     5641 actgtttttc acaaggtaaa agatcgcttc ggctttctga gtgaaactaa agtgcgtgac
     5701 ctggtggaca ggctaatcaa ggcattgacc atcaaagtct ctgatcctca tcttccggtg
     5761 aatacgcttt ctggtggtaa cgcgcagcga gtatcgatcg ccaaatggct ggcgattggg
     5821 ccacgactgt tgattctcga tagcccgaca gtcggtgttg atatcgccaa caaagcagga
     5881 atatacggca ttatcagcga tctggcggct catggcattg cggtattgat gatctgcgat
     5941 gaaattgaag aagcgtggta tcaaagccac cggattttgg taatgcaaaa aggtcagatc
     6001 acccatagct tcctgcctga cagcagttct caggccagga ttgcggaggt ggtaaatggc
     6061 tgaactgaaa aaacgccatg aattctggct ggcattactg attgtggtgc tgtttgttgg
     6121 ccttgcctgg cgcagcgacg agtttctgac attcggtaat ttgtacgatc tcgccaataa
     6181 ctatgccatg ttgactattc tcgcctgtgg tttgtttgtg gtgttgattt ccggtggaat
     6241 tgatatttcg tttccagcaa tgaccatcat tgcgcaatac ggcatggtgc tgttgctgca
     6301 aaaaattggt ggcaacttcg ctgtcgcgtt tgcactggca ggctgcatcg gcattttact
     6361 tggcttaatt aacgccttac tggttaatcg cctacgggtg ccttctatca tcatcactat
     6421 ctcgacgctg aatattttct atggcctgct gttatggttg agtaaaggtg tgtggctgta
     6481 cgactttccg ccgtggtttg agcagggggt tatgttgttc aagtacaccg atgctgatgg
     6541 ctatgactat ggccttggtc tgccgctgat cgccatgatt acggtggtgc tgctgacagc
     6601 gtttatcatg aatttcacca gtgtagggcg caaaatttat gcccttggcg ggaaccgcga
     6661 atcagccagt cgcatcggct ttagcgtgct gaaactgcaa cttttcgtct acggctatat
     6721 gggattgatg tctggcgctg cgggtgtagt gcagtcgtgg acggtgatga ctgtcgcccc
     6781 cgattctctt ctgggttatg agctgacagt actggctgcg gtggtgcttg gcggcactag
     6841 tttgctcggc gggcgcggca cgttaactgg tactttgctc ggcgtggtgt tgttggcagt
     6901 gatgcaaaac gggctaaatt tattgggagt ctcgtcttac tggcaaacat tgatcaccgg
     6961 catcatcatc gttgccagca ttagcgccac ggcgtggagt cagcatcaga accggagcct
     7021 gctatgaaaa aatcgtggcg taataacgtt gagttttact tgatcggcct gctggtgcta
     7081 actgttgccg cgttcagcat tactatgccg gagattttct ggtcgataag taatttccag
     7141 tccgtcgctt cgcaaatgcc cgtgctgggc attctggcgt tggctatggc ggtgaccatg
     7201 ctttgcggtg gcattaactt gtcaattata gccactgcta acgcctgttc actggtgatg
     7261 gcgtgggtgg cgactcagta tccaccgggt attgctacgg ttgtcgctac gttactggcg
     7321 ggcgctggcg cggcggtgat aatcggttta tgtaacggcg tattgattgc tggtattcga
     7381 gtgttgccaa ttcttgcaac gcttgggatg atgacgttgc tcaaagggat aaatattctg
     7441 gtgacaggtg gtagcgccat tgccaattat ccctcatggg tgctgtggtt gaatcatgcg
     7501 caatggttcg gtattccgct accgatgtgg ttgtttgccg cagtggcact ggggctgtgg
     7561 atattgctgg agaaaacgcc gctcggtaaa gccatttacc tgattgggtc taacgaacga
     7621 gcaacacttt actccggcat taacactcgt cgggtgctga tttgggttta tgtcatttcc
     7681 gccttgcttt gtgcggtagc ggcatttttg atgatgtcga aactcaactc ggctaaggcc
     7741 tcttatggcg aatcgtactt gctggtgtca atcctcgcgg cagtactcgg cggggttaat
     7801 ccggacggcg gcagcgggcg gataattggt atggtgctgg cgctgtttct tctgcaaatt
     7861 attgaaagcg gcttcaatat tctcggtata agcccctacc tgacgatggc cttatgggga
     7921 acgttgctgc tctgtttcat tcaggctcgc ggcatgttgg ggctggatcg ggtggtttaa
     7981 atattggcta tattcaatgg acgcgttttg ccgcgatgac atatcaggcg tcgccaaata
     8041 cacatagcta atcaggagta aacacaatga agatcaaagc tgttggttca tattccgcta
     8101 aacaaccact tgaaccgatg gatatcaccc ggcgtgaacc gggaccgaat gatgtcaaaa
     8161 tcgaaatcgc ttactgtggc gtctgccatt ccgatctcca ccaggtccgt tccgagtggg
     8221 cggggacggt ttacccctgc gtgccgggtc atgaaattgt ggggcgtgtg gtagccgttg
     8281 gtgatcaggt agaaaaacat gcgccgggcg atttggtcgg tgtcggctgc attgtcgaca
     8341 gttgtaaaca ttgcgaagag tgtgaagacg ggctggaaaa ctactgtgat cacatgaccg
     8401 gcacctataa ctcgccgacg ccggacgaac cgggccatac tctgggcggc tactcacaac
     8461 agatcgtcgt tcatgagcga tatgttctgc gtattcgtca cccgcaagag cagctggcgg
     8521 cggtggctcc tttgttgtgt gcagggatca ccacgtattc gccgctacgt cactggcagg
     8581 ccgggccggg taaaaaagtg ggcgtggtcg gcatcggcgg tctgggacat atggggatta
     8641 agctggccca cgcgatgggg gcacatgtgg tggcatttac cacttctgag gcaaaacgcg
     8701 aagcggcaaa agccctgggg gccgatgaag ttgttaactc acgcaatgcc gatgagatgg
     8761 cggctcatct gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg
     8821 acgattttac caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga
     8881 caccgcataa atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccggtt
     8941 ctatgattgg cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca
     9001 tcgtggctga tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc
     9061 tgcgtggtga tgtgaaatat cgttttgtta tcgataatcg cacactaaca gactgaaaaa
     9121 attaataaat acgctgtggt ttaacatatt aacttcgctc tccacttaac tttttagtta
     9181 aggagagcga aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa
     9241 attaactccc tccagtacct aatacttcac acgttagtta tgagcgattt ctgatagtgc
     9301 ctggtttaat cagagcttta ttatctgcga cgtttatttt tatttaagag agatcgcgat
     9361 gatatcatta aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa
     9421 tacggttaaa aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga
     9481 tatattcaag gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt
     9541 agtcgatgac gacaaactca ttcatctcat ggctgccgta cgagactgtg agtggtcaga
     9601 cgataacgca ctcaccataa atgtgcagtt taatgatttc cctggatttt atgactggat
     9661 ggattaccct gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc aaaaagggac
     9721 agtttgggta tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg tgcaggctgg
     9781 acgctttacc gggcttaaga aattagtgca gtttatcaat tcagtgcgaa cagattgcaa
     9841 atgtattctt cttgaacacc atatgccttt gctgagactt ttccctaaag ggaaagagtg
     9901 tatgcatgtg gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag acgcgcccat
     9961 taagcaggcg cttgcgcatg gattattatt acacttaaaa aatatctacc ctgtttttcc
    10021 tgaatcactg gtgatgttat tgctctctgt tctggacgta aaaacataca gagatgatgc
    10081 acgattaaat gaagggatat ctaatagagt ccaggaatta ggtgacagat attatcctgt
    10141 aaataagcac gttaaaatcc gctacactct ttagttcaga aagggttcca cctgcaagtt
    10201 gttattctac ctgagagtga atcgcaatgc aggtaatcat ttcatctgta tatctgtatg
    10261 ccaactggca ttccatctct tttttcgccg atattttgtt gtgatcggcg acacttcgct
    10321 taaaaaagca ccagtagtgg tttcgcagcc atgcggtgta taaaaaaaaa tgatctcatg
    10381 cagatgtttt gtgaatgtgt gggttgacat tcatatgaaa aaaatcataa ttccatcatg
    10441 tttgtgtggg gtcttttctg tatcttacgc atcgcactca agcctgacag aaaatatgct
    10501 gtaaggctca tatcaaaaac cgccattagc tcatcaggaa gagcagacga caaccaggat
    10561 tgttgtatgg tacggggttc gaggccccga tggcggtcca acttttcaaa actcttctga
    10621 attagatccc cttattatca tccctgcaaa gtggaacatt gccattgttt ggctattcag
    10681 gacgggtcag accaatgaaa aaagcccaca caggggagag tgggctgaaa tgggaagcta
    10741 aagactcaag taaacttatc ggaaataagg accacgcatt acgggggcta tcatcgccga
    10801 tggtcgggat tcggcaaatc aggttgatta cgtagagagt aaattatctg ctcaccgctg
    10861 cgtcacccct tcgtaaatca ggcgtagcgc gaatacacca ataattgcac caataacccg
    10921 actggcaacg cgttgcatac gcccataagc acgacgcaca gcgggcaaag aaaacgcctg
    10981 actaagaaaa actcgccaga taattgatgc gagcacaatc cccgcccagg ccattaaacg
    11041 tgcccatgtt ggtgtttcgg catttaatgt tactgagaaa atactaataa aaaataaaac
    11101 ggtttgtggg ttagagagat cggtaattaa tccacggcga aaaaagacat accagggggc
    11161 gttaatcggt tgttgtagtg tgctcatttg cggtgttgac tggcggcgca tgctgcacca
    11221 cgcaaaccat aagagataag cgccgccgac gattctgata agcgaaaaaa tctcctcaca
    11281 ctgcgtaatt agcgttgcaa gaccaaacaa acccaacccg gaataaaatg catcgcccag
    11341 cgccacgccc agtccggtca gcactcctgc gcgtcgaccg gaagccaggc tggtttgtac
    11401 taccacaaaa agattggctc ccggattaaa aaaagtaatc acgaacagtc ctacggtcag
    11461 gtaaacggca tgcaaaggat ccatagtgat ttcatccata aataagtgaa ctaactgcat
    11521 catatttcta ccaaaaataa tcgggtgcga gagagatcac aaagtgtctt atttccggtt
    11581 actggcgttt atgccctgac tgaactaatt attaatcaac ccaataatgt cggtgggtga
    11641 tagtgtgata acaactctgg agccgtaata tgaaaataat ctctaaaatg ttagtcggtg
    11701 cgttagcgtt tgccgttacc aatgtctatg ccgctgaatt gatgaccaaa gcggaatttg
    11761 aaaaagttga atcgcagtat gaaaaaatag gtgatatttc aaccagtaat gaaatgtcga
    11821 ctgcagatgc aaaagaagat ttaatcaaaa aagccgatga aaaaggggct gatgtgttgg
    11881 tactgacctc cggtcaaact gacaataaga tccacggcac ggcggatatt tataagaaga
    11941 agtaattctg aatcctatgt aaacatcccc gatgcgtaag tttatcggtg atcatctatt
    12001 gaaatttatg ccggataaag cgttcgcgct gcattcggca gttcagcttt tcagccgccg
    12061 ccagaacgtc gtccggctga tgcctaaata attcgccgct gctgttttat cgccattaaa
    12121 tttctccagt gcctgttgtg gtgtcagcaa gcgtggagcg ggaattttcg ccgactcgcg
    12181 cgccagttcc ggcagtagca gctgcaaaaa ctgcggcgtt aaatccggcg tcggttccac
    12241 acttaaaaat agcgccagtc gctccatcat attgcgcagt tcacgaatat tacctggcca
    12301 gtcgtagtgc accagcacgg tttcgcttgc ctgtaatccc tggcgtaatg cggcagaaaa
    12361 tggggcggag agcgccgcca gagacacttt caaaaagctt tccgccagcg gaagaatatc
    12421 tgccacccgc tcgcgcagtg gtggcaattg cagacgcaaa atactcagcc gataaaacag
    12481 atcacggcga aactgccctt gccgcatatc ttcttccaga ttgcagtgag tggcgctaat
    12541 gacccgtaca tctaccggaa caggctgatg cccgccgacg cgggtgacct ctttttcttc
    12601 cagcacccgt aacaggcgag tctgcaaagg tagcggcatt tcgccaatct catccagaaa
    12661 cagcgtgcca ccgtgggcaa tttcgaacag cccggcgcga cctccgcgtc gcgagccggt
    12721 aaacgcccct tcctcatagc caaacagctc cgcttccagc agcgattcgg caatcgcccc
    12781 gcagttgacg gcaacaaacg gatgcgactt tttgccctgt cgcgcatcgt ggcgggcaaa
    12841 atattcccga tgaattgcct gggccgccag ctctttgccc gtccccgttt ccccctcaat
    12901 caacaccgcc gcactggagc gggcatacag caaaatagtc tgccgcacct gctccatctg
    12961 tggtgattga ccgagcatat cgcccagcac gtaacgagta cgcagggcgt tgcgggtggc
    13021 atcgtgagtg ttatggcgta acgacatgcg cgtcatatcc agcgcatcgc tgaacgcctg
    13081 gcgcacggtg gcggcggaat agataaaaat tccggtcatt ccggcttctt ctgccagatc
    13141 ggtaatcagc cccgcgccga ccaccgcttc ggtgccgtta gcttttagct cgttaatctg
    13201 cccgcgtgcg tcttcttcgg taatgtagct acgttgatcg aggcgcaaat taaaggtttt
    13261 ttgaaacgcc accagtgccg gaatagtttc ctgataagtg acaacgccga tagaagaggt
    13321 gagttttccg gcttttgcca gtgcctgtaa cacatcgtag ccgctcggtt taatcaaaat
    13381 aactggcact gacaggcggc ttttcaggta cgcgccgtta gagccagcgg cgatgatggc
    13441 gtcacagcgt tcgtttgcca gtttcttgcg gatgtaggtc actgcttttt caaagccaag
    13501 ctgaataggg gtaatgttcg ccaggtgatc aaactcgagg ctgatatcgc gaaacagctc
    13561 gaataagcgc gttacagata ccgtccagat aaccggtttg tcgtcattca gccgtggtgg
    13621 atgtgccata gcgcaccgca aagttaagat accgaatatt gggtttagtc ttgtttcata
    13681 attgttgcaa tgaaacgcgg tgaaacattg cctgaaacgt taactgaaac gcatatttgc
    13741 ggattagttc atgactttat ctctaacaaa ttgaaattaa agatttaatt ttattaaggc
    13801 aattgtggca caccccttgc tttgtcgtta tcaacgaaaa taacaagttg ataacaaagg
    13861 atgggctatg tctctacact ctccaggtaa agcgtttcgc gctgcactga ctaaagaaaa
    13921 tccattgcag attgttggca ccattaacgc caaccatgcg ctgttggcgc agcgtgccgg
    13981 atatcaggca atttatctct ccggcggtgg cgtggcggca gggtcgctgg ggctgcccga
    14041 tctcggtatt tctactcttg atgacgtgtt gacagatatt cgccgtatca ccgacgtttg
    14101 ttcgctgccg ctgctggtgg atgcggatat cggttttggt tcttctgctt ttaacgttgc
    14161 gcgtacggtg aaatcgatga ttaaagccgg tgcggcggga ttgcatattg aagatcaggt
    14221 tggtgcaaaa cgctgtggtc atcgcccgaa caaagcgatc gtctcgaaag aggagatggt
    14281 ggatcggatc tgcgcggcgg tggatgcgaa aaccgatcct gattttgtga tcatggcgcg
    14341 caccgatgcg ctggcggtag aggggctgga tgcggcgatc gagcgtgcgc aggcctatgt
    14401 tgaagcgggt gccgagatgt tgttcccgga ggcgattacc gaactcgcca tgtaccgcca
    14461 gtttgccgat gcggtgcagg tgccaatcct cgccaacatt accgaatttg gcgccacgcc
    14521 gctgtttacc accgacgaat tacgcagcgc tcatgtcgcg atggcgctgt acccgctttc
    14581 agcgtttcgc gccatgaacc gcgccgccga acatgtctac aacgtcctgc gccaggaagg
    14641 cacgcagaaa agcgtcatcg acaccatgca gacccgcaac gagctgtacg aaagcatcaa
    14701 ctactaccac tacgaagaga agctcgacga cctgtttgtc cgtagccagg cgaaataaaa
    14761 aacgcccgtt ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc gacttatcag
    14821 gcctacgggg tgaactgtag gtcggataag acgcatggcg tcgcatccga caacaatctc
    14881 gaccctacaa atgataacaa tgacgaggac aatatgagcg acacaacgat cctgcaaaac
    14941 agtacccatg tcattaaacc gaaaaaatct gtggcgcttt ctggcgttcc ggcgggcaat
    15001 acggcgctct gcaccgtggg taaaagtggc aatgacttgc attaccgtgg ctacgatatt
    15061 cttgatctgg cggaacattg cgaatttgaa gaagtggcgc atctgctgat ccacggcaaa
    15121 ctgccgaccc gtgacgaact cgccgcctac aaaacgaaac tgaaagccct gcgcggttta
    15181 ccggctaacg tgcgtaccgt gctggaagcc ttaccggcgg cgtcacaccc gatggatgtt
    15241 atgcgcaccg gcgtttccgc gctcggctgc acgctgccag aaaaagaggg gcataccgtc
    15301 tctggcgcgc gggatattgc cgacaaactg ctggcgtcac ttagctcgat tcttctctac
    15361 tggtatcact acagccacaa cggcgaacgc atccagccgg aaaccgatga cgactccatc
    15421 ggtggtcact tcctgcatct gctgcacggc gaaaagccgt cgcaaagctg ggaaaaggcg
    15481 atgcatattt cgctggtgct gtacgccgaa cacgagttta acgcctccac ctttaccagc
    15541 cgggtgattg cgggcactgg ctctgatatg tattccgcga ttattggcgc gattggtgca
    15601 ctgcgcgggc cgaagcacgg cggggcgaat gaagtgtcgc tggagatcca gcaacgctac
    15661 gaaacgccgg acgaagccga agccgatatc cgcaagcggg tggaaagcaa agaagtggtc
    15721 attggttttg gtcatccggt ttataccatc gccgacccgc gccaccaggt gattaaacgt
    15781 gtggcgaagc agctctcgca ggaaggcggc tcgctgaaga tgtacaacat cgccgatcgc
    15841 ctggaaacgg tgatgtggga gagcaaaaag atgttcccca atctcgactg gttctccgct
    15901 gtttcctaca acatgatggg cgttcccacc gagatgttca caccactgtt tgttatcgcc
    15961 cgcgtcaccg gctgggcggc gcacattatc gaacaacgtc aggacaacaa aattatccgt
    16021 ccttccgcca attatgttgg accggaagac cgccagtttg tcgcgctgga taagcgccag
    16081 taaacctcta cgaataacaa taaggaaacg tacccaatgt cagctcaaat caacaacatc
    16141 cgcccggaat ttgatcgtga aatcgttgat atcgtcgatt acgtcatgaa ctacgaaatc
    16201 agctccaaag tagcctacga caccgcacat tactgtctgc tcgacacgct cggctgcggt
    16261 cttgaagctc tcgaataccc agcctgtaaa aaactgctgg ggccaattgt ccccggcacc
    16321 gtcgtaccta acggcgtgcg cgtccccgga actcagttcc agctcgaccc cgtccaggcg
    16381 gcatttaaca tcggcgcgat gatccgctgg ctcgatttca acgatacctg gcttgcggca
    16441 gagtggggcc atccttccga caatctcggc ggcattctgg caacggcgga ctggctttca
    16501 cgcaacgcgg tcgccagcgg caaagcgccg ttgaccatga aacaggtgct gaccggaatg
    16561 attaaagccc atgaaattca gggctgcatc gcgctggaaa actcctttaa ccgcgtcggc
    16621 ctcgaccacg ttctgttagt gaaagtggct tccaccgccg tggtcgccga aatgcttggc
    16681 ctgacccgcg aggaaattct caacgctgtt tcgctggcgt gggtggacgg tcagtcgctg
    16741 cgcacctatc gccatgcgcc gaacaccggc acgcgtaaat cctgggcggc gggcgatgcc
    16801 acttcccgcg cggtacgtct ggcactgatg gcgaaaacgg gcgaaatggg ttacccgtca
    16861 gccctgactg cgcctgtgtg gggcttctac gacgtctcct ttaaaggtga atcgttccgc
    16921 ttccagcgtc cgtacggttc ttacgtgatg gaaaatgtgc tgttcaaaat ctccttcccg
    16981 gcggagttcc actcccagac ggcagttgaa gcagcgatga cgctctatga acagatgcag
    17041 gcagcaggca aaacggcggc agatatcgaa aaagtgtcca tccgcaccca cgaagcctgt
    17101 attcgcatca tcgacaaaaa ggggccgctc aataacccgg cagaccgcga ccactgcatt
    17161 cagtacatgg tggcgatccc actgctattc gggcgcttaa cggcggcaga ttacgaggac
    17221 aacgttgcgc aagataaacg cattgacgcc ctgcgcgaga agatcaattg ctttgaagat
    17281 ccggtattta ccgctgacta ccacgacccg gaaaaacgcg ccatcgccaa tgccattacc
    17341 cttgagttca ccgacggcac acgatttgaa gaagtggtgg tggagtaccc cattggtcat
    17401 gctcgccgcc gtcaggatgg tattccgaaa ctggtcgata aattcaaaat caatctcgcg
    17461 cgccagttcc cgactcgcca acagcagcgc attctggagg tttctctcga cagagctcgc
    17521 ctggaacaga tgccggtcaa tgagtatctc gacctgtacg tcatttaagt aaacggcggt
    17581 aaggcgtaag ttcaacagga gagcattatg tcttttagcg aattttatca gcgttcgatt
    17641 aacgaaccgg agcagttctg ggccgagcag gcccggcgta ttgactggca gacgcccttt
    17701 acgcaaacgc tcgatcacag caatccgccg tttgcccgtt ggttttgtga aggccgaacc
    17761 aacttgtgcc acaacgctat cgaccgctgg ctggagaaac aaccagaggc gctggcgctg
    17821 attgccgtct cttcggaaac agaagaagag cgcaccttta cctttcgcca gcttcatgac
    17881 gaagtaaacg cggtggcgtc aatgctgcgc tcgctgggcg tgcagcgtgg cgatcgggtg
    17941 ctggtgtata tgccgatgat tgccgaagcg catattactc tgctggcctg cgcgcgcatt
    18001 ggcgctattc actcggtggt gtttggggga tttgcctcgc acagcgtggc ggcgcgaatt
    18061 gatgatgcta aaccggtgct gattgtctcg gctgatgccg gagcgcgcgg tggcaaaatc
    18121 attccctata aaaaattgct cgacgatgcg ataagtcagg cgcagcacca gccacgccat
    18181 gttttgctgg tggatcgcgg gctggcgaaa atggcacgcg tcagcgggcg ggatgtcgat
    18241 ttcgcgtcgt tgcgccatca acacatcggc gcgcgggtac cggtggcgtg gctggaatcc
    18301 aacgaaacct cctgcattct ctacacctcc ggcacgaccg gcaaacctaa aggcgtgcag
    18361 cgtgacgttg gcggatatgc ggtggcgctg gcgacctcga tggacaccat ttttggcggc
    18421 aaagcgggcg gtgtgttctt ttgtgcttcg gatatcggct gggtggtggg gcattcgtat
    18481 atcgtctacg cgccgctgct ggcggggatg gcgactatcg tttacgaagg attaccgacc
    18541 tggccggact gcggcgtgtg gtggaaaatc gtcgagaaat atcaggttag ccggatgttc
    18601 tcagcgccga ccgccattcg cgtgctgaaa aaattcccta ccgctgaaat tcgcaaacac
    18661 gatctctcgt cgctggaagt gctctatctg gctggagaac cgctggacga gccgaccgcc
    18721 agttgggtga gcaatacgct ggatgtgccg gtcatcgaca actactggca gaccgaatcc
    18781 ggctggccga ttatggcgat tgctcgcggt ctggacgaca ggccgacgcg tctgggaagc
    18841 cccggcgtgc cgatgtatgg ctataacgtg cagttgctta atgaagtcac cggcgaaccg
    18901 tgtggcgtca acgagaaagg gatgctggtg gtggaagggc cgctgccgcc ggggtgtatt
    18961 cagaccatct ggggcgacga cgaccgcttt gtgaagacgt actggtcgct gttttcccgc
    19021 ccggtgtacg ccacctttga ctggggcatc cgtgacgctg acggttatca ctttattctc
    19081 gggcgcactg acgatgtgat taacgttgcc gggcatcggc tggggacgcg cgagattgaa
    19141 gagagtatct ccagtcatcc gggcgttgcc gaagtggcgg tggttggggt gaaagatgcg
    19201 ctgaaagggc aggtggcggt ggcgtttgtc attccgaaag agagcgacag tctggaagat
    19261 cgtgatgtgg cgcattcgca agagaaggcg attatggcgc tggtggacag ccagattggc
    19321 aactttggcc gcccggcgca cgtctggttt gtctcgcaat tgccaaaaac gcgatccgga
    19381 aaaatgctgc gccgcacgat ccaggcgatt tgcgaaggac gcgatcctgg ggatctgacg
    19441 accattgatg atcctgcgtc gttgaatcag atccgccagg cgatggaaga gtaaggtatt
    19501 gtcggatgcg tcgcgtggcg catccgacag ttgtgcgcag atgcctgatg cgacgc
//
LOCUS       DAZFTN010000085        18986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFTN010000085 DAZFTN010000000
VERSION     DAZFTN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..407)
                     /gene="yhaH"
                     /locus_tag="ACHKV2_003450"
     CDS             complement(42..407)
                     /gene="yhaH"
                     /locus_tag="ACHKV2_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6941274.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(699..1685)
                     /gene="yqjG"
                     /locus_tag="ACHKV2_003451"
     CDS             complement(699..1685)
                     /gene="yqjG"
                     /locus_tag="ACHKV2_003451"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO6941275.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1755..2147)
                     /gene="yqjF"
                     /locus_tag="ACHKV2_003452"
     CDS             complement(1755..2147)
                     /gene="yqjF"
                     /locus_tag="ACHKV2_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6941276.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLQTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2333..2632)
                     /gene="yqjK"
                     /locus_tag="ACHKV2_003453"
     CDS             complement(2333..2632)
                     /gene="yqjK"
                     /locus_tag="ACHKV2_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO6941277.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2622..3026)
                     /gene="yqjE"
                     /locus_tag="ACHKV2_003454"
     CDS             complement(2622..3026)
                     /gene="yqjE"
                     /locus_tag="ACHKV2_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6941278.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3029..3334)
                     /gene="yqjD"
                     /locus_tag="ACHKV2_003455"
     CDS             complement(3029..3334)
                     /gene="yqjD"
                     /locus_tag="ACHKV2_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO6941279.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3372..3740)
                     /gene="yqjC"
                     /locus_tag="ACHKV2_003456"
     CDS             complement(3372..3740)
                     /gene="yqjC"
                     /locus_tag="ACHKV2_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO6941280.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3887..4270)
                     /gene="mzrA"
                     /locus_tag="ACHKV2_003457"
     CDS             complement(3887..4270)
                     /gene="mzrA"
                     /locus_tag="ACHKV2_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO6941281.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4274..4936)
                     /gene="yqjA"
                     /locus_tag="ACHKV2_003458"
     CDS             complement(4274..4936)
                     /gene="yqjA"
                     /locus_tag="ACHKV2_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO6941282.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4958..5041)
                     /gene="yqiM"
                     /locus_tag="ACHKV2_003459"
     CDS             complement(4958..5041)
                     /gene="yqiM"
                     /locus_tag="ACHKV2_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO6941283.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5281..6057)
                     /gene="exuR"
                     /locus_tag="ACHKV2_003460"
     CDS             complement(5281..6057)
                     /gene="exuR"
                     /locus_tag="ACHKV2_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO6941284.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6187..7485)
                     /gene="exuT"
                     /locus_tag="ACHKV2_003461"
     CDS             complement(6187..7485)
                     /gene="exuT"
                     /locus_tag="ACHKV2_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO6941285.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7968..9380
                     /gene="uxaC"
                     /locus_tag="ACHKV2_003462"
     CDS             7968..9380
                     /gene="uxaC"
                     /locus_tag="ACHKV2_003462"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO6941286.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9395..10882
                     /gene="uxaA"
                     /locus_tag="ACHKV2_003463"
     CDS             9395..10882
                     /gene="uxaA"
                     /locus_tag="ACHKV2_003463"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO6941287.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10965..11516
                     /gene="ygjV"
                     /locus_tag="ACHKV2_003464"
     CDS             10965..11516
                     /gene="ygjV"
                     /locus_tag="ACHKV2_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO6941288.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPIELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11521..12765)
                     /gene="sstT"
                     /locus_tag="ACHKV2_003465"
     CDS             complement(11521..12765)
                     /gene="sstT"
                     /locus_tag="ACHKV2_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO6941289.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13164..14129)
                     /gene="alx"
                     /locus_tag="ACHKV2_003466"
     CDS             complement(13164..14129)
                     /gene="alx"
                     /locus_tag="ACHKV2_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO6941290.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14413..15399)
                     /gene="ygjR"
                     /locus_tag="ACHKV2_003467"
     CDS             complement(14413..15399)
                     /gene="ygjR"
                     /locus_tag="ACHKV2_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6941291.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15478..16170)
                     /gene="ygjQ"
                     /locus_tag="ACHKV2_003468"
     CDS             complement(15478..16170)
                     /gene="ygjQ"
                     /locus_tag="ACHKV2_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO6941292.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16247..16750)
                     /gene="ygjP"
                     /locus_tag="ACHKV2_003469"
     CDS             complement(16247..16750)
                     /gene="ygjP"
                     /locus_tag="ACHKV2_003469"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO6941293.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16835..17971
                     /gene="rlmG"
                     /locus_tag="ACHKV2_003470"
     CDS             16835..17971
                     /gene="rlmG"
                     /locus_tag="ACHKV2_003470"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO6941294.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18255..18569
                     /gene="higB"
                     /locus_tag="ACHKV2_003471"
     CDS             18255..18569
                     /gene="higB"
                     /locus_tag="ACHKV2_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO6941295.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18566..18982
                     /gene="higA"
                     /locus_tag="ACHKV2_003472"
     CDS             18566..18982
                     /gene="higA"
                     /locus_tag="ACHKV2_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO6941296.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4857 a         4914 c         4815 g         4400 t
ORIGIN      
        1 taaaagccgg atgagtcaga catccggcga gcagatttgt tttaaggttc cagttttgga
       61 tcaggtccaa agcggttttc gcctggcgta cccgcctggc agttaaagac gatgataata
      121 agccagccga taaacgggat taagaatagc agtgcccacc acgccgagcg gtcggtgtcg
      181 tgcaggcggc ggaactgaac tgcccaccac ggtaaaaaca ccagaatacc gtagattgtc
      241 gtcaggatgc cttcgccacc agcgcgttgc cagcctaaca ttttatccag cagccccagc
      301 acgaacgtaa agatgatgtt gaccagaata aacatccagt actctttgcg ccgcgcacga
      361 ccccggaaac cgacataatt ttttagtact ttcagatacc agtccatttt tgcctcaatt
      421 ttgctcaatc acttgttttt aaagcagcaa attaagaata gacgtgaatg aaatggcagt
      481 aaatattcgt tcaaggaatt aataaagtgc tgtgaaggcg cgctaagact gatgacaagc
      541 gcaaaattgc cagatgcgct acgcttatct ggcccgcgcg gtcattgcaa catattgaat
      601 ataaaagttt tgtaggtttg ataaggcgtt catgccgcat ccgacatgaa caacatgcac
      661 ttagctaatt atcagaggca cctcgtaagg tgcttttttt aacctaagcg aacatctcgt
      721 ccatgcggtt catcgagatc ctgccacgga ccaattgaaa taatccgcgt agggttgatg
      781 gtcttatgac tgcggaagta atgattacgg atatgatcga aattgactgt ttcggcaatt
      841 cccggcatct ggtagatatc acgcaggaag ccatacagat taaggtaatc gctgatgcgg
      901 tgcttatcac acttgaagtg ggtcacatac actggatcaa aacggaccag cgtggtccac
      961 aggcgaatat cggcttcggt tagctggttg ccggtcaggt aacgatgctg acctaaaatc
     1021 tgttccagtc gcgccagcga ttcaaacact ttctccaccg cttcgtcgta ggcttgctgg
     1081 ctggtggcaa aaccagcttt atacacgccg ttgttaacgg tgtcataaat ccagccgtta
     1141 agttcgtcaa ttttcgtttg cagggctggt gggtagtaat cacccgcttt cgcgcccagc
     1201 gcatcaaacg cggtattaaa catgcggatg atttctgctg attcgttgct gacgatggtg
     1261 tggttctttt tgtcccacag cacgggaaca gtaactcgtc cgctgtagtg tggatcggcg
     1321 tggagataaa gctgatacaa aaattcatgt tgatagagcg tgtctccggt tgctcccgga
     1381 aaactgtcat caaaggtcca gccgttttcc agcatcagcg ggttcactac ggaaacggaa
     1441 ataaacggtt ccagtccttt gagtttgcgc atgatcagcg tgcggtgcgc ccacgggcag
     1501 gcgagtgaaa catagagatg ataacgatct ttctctgcga taaaaccgcc tgtgccagtg
     1561 gggccaggag cgccatcggc agtgagccag ttacgaaatg cggaagctga acgttgaaat
     1621 ttaccgccgg tagatttggt atcgtaccag gtgtcatgcc agacgccgtc aatcagttga
     1681 cccattgttc tctcctccaa tagcaaaaac gaggagcttc ctcctcgttt tttatcgtta
     1741 agtatagcgc gtgcttacca ctttttattc agcaggcggt cgatgctata cgcgcccgga
     1801 ccggtaattg ccagcagcag gaatccgcca gaaattgtca ggtttttcat gaacatcagc
     1861 gagttgacgc cttcagcaaa gttgctgtga aataaaaatg ccgtctgcag cgtaaagccc
     1921 gcagtaaaca gggctgtggt gcgagtcagg aaaccgaaca ggattgccag accaccacca
     1981 aactcaagca gaatcaccag cggcagcata aaacccggga cgcccatcgc ttccatatat
     2041 tgttgggtac ccgcgtaacc agtaattttt ccccagcctg cggtaataaa cagaatcggc
     2101 attaaaatgc gcgctaccag tacaccaaca tcttctaatt ttttcatcat actctccact
     2161 gcgccactca ggctgctgat tgtttttttg tgcaagggcg cggtattagc gtcgttgctg
     2221 tcgatggaga gaatcataaa cgtggtgaat gatgattgtt agcaaggaaa actgtcaaaa
     2281 atcttcaaaa aatttgatgg gtaaggccgg aatggctccg gccagaggga agttaaccgc
     2341 gaagctgttg ctgcttgagg gtcgttttaa ccagacgcca ggcgctccat acgccaaaac
     2401 cgcgtctggc ccagcggacc agcatattag gatggcgaat cgtccagatc gccatcacgc
     2461 tactgccaac cagcgcccag gagcgcagac ttagcagcat attccagcga cgatcgtaag
     2521 cgcctgttgc ctccagccat tcacgacgac tggcagaaag atccagccgt tgttgctgga
     2581 tctggctaag cagttgcgcc ttacgtcgtt cacgttcgac tttactgctc acgggactcc
     2641 tcctcgagca gctgccgatc gtttgccagc tcatggcgtg tatggcgcag caaggtggat
     2701 ttacgcgatt tacgtaacgt ccagataccg ccaatcagtg ccagtagcag caacaccacg
     2761 gtggtggcaa tcatcgcatt caggcgatat tgcggatcaa ccgcccaaat aattagcacc
     2821 atcaggctca taagaccaaa tgcagcgaaa agcatcgtca ggcccagcat cagtaaaagt
     2881 tgaaagagat tagctttttc ctcttccagc tcaaccaccg ccagccgcag acgtgtttcc
     2941 accatttcaa ccatgatgga aacaattcgc tgcccgatgc ccagaacgct tttaccgggc
     3001 ccttgcgcgt gatgagtgtc cgccataatt aacgacgcga cagcagaacg ccgagcacta
     3061 caccgattgc agcgccaatg cccacgcccg tccatggatt ttcacgcaca tactcatcgg
     3121 cacgcgccgc cgcgacacgg gtttgtttgg cgatggcatc accggtttca cccaggcgat
     3181 aacggctctg tttcagtgcc tgctccgctt tgctacgaat cttactcaac tcttctttcg
     3241 acttctcgcc agatgagcta agcacctctt ccagcgtatc ggaaagggat ttcaactcag
     3301 cacgcagatg ttccgtagtg tgttctttcg acatagtttt ctccaggtga gtaagtagtg
     3361 actattgtga gttagtagtc gcgcgcttcc agctttttca gctcttcctg cgcttctgcc
     3421 agtttccgtt cgcgtttggc aatcttatcg gcatcgcctt tttgcttcgc ctcggctaaa
     3481 tcttgctggc gttccgccac ctcatctttc tgctttgcga ttttcttctg atgatcggca
     3541 cgcagctggc tatctgaaca gttggcccgg acttcactca gggctttatt cagaccgtca
     3601 atacgattct ggttttggtg tttttcggca tagctgatct ccttaaggat attttgctcc
     3661 ttttcctgac acagggtagt ggcataacta ccggcactaa gagcaaaaag agaaacagct
     3721 aaagcgatgc ggtatttcat tcttgaacct tccattgtga ttcggaccta ttacaaagga
     3781 acgattgtcc agacaacgaa gcggtagtgt taaccaccgc ctcattaatc atagacagtt
     3841 acggtgaaat caccaaagtg agtgatctgt ttcggacaac aggaagttat ccgaagcgat
     3901 gagagttatc ccgtagccgg gtcagccact gcatagcctg actgttattg tcctgctgcg
     3961 caatgatgta gccttggggc aatgttctgt cgaggaccgc ttttgcagcg gcgctctggt
     4021 cgctggagtc aaaggtaatt aacagagtgt cgtttttggg ggtgatactt ttgaaaggaa
     4081 tgccatgagc gtcaaggtga tgccagattg aaaaaccgtc tggcatcgtt gtgccttgat
     4141 gaacggcacg aatcgccagc gtagactctt gctggcgaac cgcggaccag gccagcaaca
     4201 gcgtgccgac cagaagtaaa acagcgccgg accaggctag ctggcgaagc gacatgcgag
     4261 gtatttgcat cccttacccc cgatttccat atttcttttt ccataatacg accagagaac
     4321 ctgccaggcc aaacaccagc agcaccaccg ggagcagcat caggcatgac atcagctggt
     4381 cctcgtactt taaaaatacc ggcgttttgc cgagcatgta acccagagtt gtcaggatca
     4441 atacccacag cagaccgctc atccagttga aaaactgaaa gcgcgcgtta ttcagccctg
     4501 ataacccggc aatcgtcggc agcagtgttc tgacaaacgc aataaagcga ccaattaaca
     4561 gcgccgataa accgtgttta tgaaaaagat ggtgtgcgcg ttgatgataa tgcgcgggta
     4621 aatgagatag ccagttttgt acggtgcggg tattgcccag ccatcgcccc tgaatatagc
     4681 tgacccagca gccgaggctg gcggcaacgg tcagcagcag aatcgtttgc ggatagccca
     4741 tcgcgccttt cgcaatcaac acgccgacca ataccagtaa actgtcgccc ggtaaaaagg
     4801 ccgccggaag caagccgttt tcaaggaaca aaattacaaa caagacaaaa tacaacatgc
     4861 caatcatcga tggattggcc agggtttcaa aatcctgcgc ccacagggct tgcagcaatt
     4921 gggtcaaaag ttccattcat tattcctggt gaatcggtta acgccacgct ggttttaaaa
     4981 gatgcactac cgcaatgtta ttgctgtttt ggtatgttct gcgggtttga tacatcaaca
     5041 cagcctgaac tattccctgt taaatcgtga gataagaaag cattcaaaaa cataaattca
     5101 aattgaatct aattgtaaca aaggtcaacg ttgttcgtta tcaaaattac atgtccgtgg
     5161 agtctgattt tgctgaatgc aggggagggt ggcgagagga tttacacagg ctgacacttt
     5221 atacttttcg cttaccgacg agacaaacag gcgcgtcgca gcgcctgtcg gacaggtatt
     5281 tcatttactg ccactggtgg ccgtatcgag atgcactacc gggttttcgg cgaacagata
     5341 gcggtcggca ttgaactcga agtcgtcgct ggtttcgttg aataacatga tcttggtgtt
     5401 ttccaggtgc tgccacattg ccagcttagc agcatgagga tctttgcgaa tcagcgcctt
     5461 gaggatttga tcgtggtcat cacaccagtt atcgacggta cgggaatcaa tgtgttcgtg
     5521 cagttttttc cagtacgggt tatggctacg ctgggtccac attttttcaa cgatagccgc
     5581 cagggcggag ttctgcgtcg ccagggctac ctgaatatgg aactgcaaat cccactcgga
     5641 atcacggaag cattgttcgc cgcgcgcctg ctcctggatg gccatcaatt tcatgatgtc
     5701 ctgtttcgtt acctgagttg ctgcgaactc ggcaatatta ctttcgatga gttggcgagc
     5761 ctggagtaac tcaaacggac cgtaattggc gaattccata ttattgtccg cagcctgctg
     5821 atggcgcggc tggttggaaa ccacgtgaat acctgaacct ttacgcactt cgacatagcc
     5881 ttcaacttcc agcatgatga tggcttcacg aactaccgta cggctgacgt tcttttcatc
     5941 ggcaataaag cgctcagcag gcagtttatc acccaccaga tagacgccct gttcgatgcg
     6001 ctctttcagg tcagcggcaa gttgttgata caaacggcgt ggttcagtga tttccatatg
     6061 cgctccaggc atgatacggc agactatatt tgttatacca cttttgcgag tttagctcta
     6121 ctcgcagtga tgaaaaagcc gcctgagaag gcggctttga agcgaaacta aaacttacac
     6181 gaagcgttaa tgttgcggtg cgggatcgtt atgtgtttcc tgcgccacct caatcgccgg
     6241 tttgttctgc aatacggtcc agataaccag cgcacccagc aggtcaaata ctgccagaac
     6301 tgcgaacagc gggctgaagc cgatggtgtc agccagtgca ccgacaacca gcgcaaacag
     6361 cgtacttgcc agccatgcgg acatcccggt taaaccgttc gccgttgcca cttcgttacg
     6421 accaaacacg tcggaagaga gcgtaatcag cgcgccagac agtgcctggt gggcaaaacc
     6481 accgatacac agcagcataa ttgcgacata cgggttggtg aacaggccga tcatacccgg
     6541 gccaatcatc agcactgcac ccagcgttac gaccatctta cgggaaacga tcaggttcac
     6601 accaaaccaa cgctggaaca gcggcggcag gtaaccaccg aggatacaac cgaggtcagc
     6661 aaacagcatc ggcatccagg cgaacatcgc gatctctttc aggttaaagc cgtaaacttt
     6721 aaacatgaac agcgggatcc acgcgttaaa agtaccccag gccggttctg ccaggaaacg
     6781 cggcagcgcg ataccccaga actgacggtt acgcaggatc tgaccaacgg acattttctt
     6841 cgccgtactc acctggtgct gggcttcctg accattaata atatagtcgc gttcttcatc
     6901 ggtcagatgc ttctggtcgc gcggatgttt atagaaaatc agccatgcca tcgcccagat
     6961 aaagctcaat gcaccggaga tgataaatgc catctgccag ctgtgcatca cgattgccca
     7021 taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa aatagcctac
     7081 tgcgatggaa cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccgggat
     7141 catcgctgct tccgcggcac cgaccgcacc acgagcaaca gccaggccac cccagctacc
     7201 tgccagcgcg gttgcaccac agaacacggc ccacagcaca gcaaacattg cataaccgat
     7261 tttcgtaccc agcacatcca gtacataacc tgctaccggt tgcatgaccg tataagcagc
     7321 agaataggct gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag
     7381 agttggcgca gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt
     7441 caccagtgcg atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt
     7501 tttgttatat ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac
     7561 gaccgggtaa ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag tttcgtgcta
     7621 attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc ggcaatttgc
     7681 attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa ctttaaaagg
     7741 tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac agcgaggcca
     7801 gtactggcgc ggcctgcaga gagatttacc actttgagag taattttttt aactacgttt
     7861 attgatctaa ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata actttgtcag
     7921 catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg actccgttta
     7981 tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac gactacgcaa
     8041 aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt gcggaagact
     8101 atcgttttaa aaacctgtat gacatctggc tgaaaggcga tcactacaaa tggcgcgcta
     8161 tgcgtaccaa cggtgtggcc gagcgtctgt gtaccggtga tgcgtctgac cgtgaaaaat
     8221 ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac cactggacgc
     8281 acctcgaact gcgtcgtccg tttggtatca ctggcaaatt gctttctccg tcaactgccg
     8341 atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc gcgcgcggca
     8401 tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc gattctctgg
     8461 agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg ccgagctggc
     8521 gtccggacaa agccttcaac atcgaacagg cgacctttaa cgactacatg gcgaagctgg
     8581 gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc ttgaccaaac
     8641 gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg gacgtggtga
     8701 tgtttgctga agcgaacgaa gcggaattgg acagcattct ggcgcgccgt ctggctggcg
     8761 aaaccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg ttccttggtg
     8821 ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg cgtaataaca
     8881 acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc aacgaccgtc
     8941 cgatggcaga agagttgtct aagctgctga gcaagcagaa cgaagaaaac ctgctgccga
     9001 aaaccattct gtactgcctg aacccgcgcg ataacgaagt gctgggcacc atgagcggta
     9061 acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg
     9121 atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt ttgctgagcc
     9181 gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt cacgaatact
     9241 tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg
     9301 acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg cgtgactact
     9361 tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga tccatgcgct
     9421 ggataacgtc gcggtcgctt tagcggattt ggctgaaggc acagaagtca gtgtcgataa
     9481 ccaaactgtt aggctgcgcc aggatgttgc tcgtggacat aaatttgcgt taacgaatat
     9541 cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat tggcggatat
     9601 tgcggcgggg gaacacgttc acgcccacaa tacgcgcacg aatctgagcg atctggatca
     9661 gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca
     9721 gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga tcctgccaac
     9781 cgtgggctgt gtcaacggca tcgcgcggca gatccagaac cgttttctga aagagaccaa
     9841 caacgctgaa ggtaccgacg gcgtgttcct cttcagccac acctacggct gctcacagct
     9901 gggcgacgat cacattaata cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc
     9961 aggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg cattccgtga
    10021 aacgctgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac agcaggatga
    10081 tgagatcgaa gccggaatcg agcatttgca tcagctgtat aacgtgatgc gcaacgataa
    10141 acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg
    10201 tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg tgattgctaa
    10261 cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat
    10321 ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca acgacttcaa
    10381 acagtacttt attgcccatg accagccgat ctatgaaaac ccatcgccgg ggaacaaagc
    10441 gggcggtatc accacgctgg aagataaatc ccttggctgt acccagaaag cgggttccag
    10501 cgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccggggc tgaacttgtt
    10561 aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat
    10621 ggtgctgttc agtactggtc gtggcacgcc atacggcggc tttgtaccga cggtgaaaat
    10681 cgctaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg acgcgggtca
    10741 gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgata ccatcgttga
    10801 gtttgccaac ggtaaacaaa cctgcaacga gcgtaacgac ttccgcgaac tggcgatctt
    10861 caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata
    10921 atgtgaagcg ccgtttgctt ttccatacct gataaggatt tatcatgacc gcgtattggc
    10981 tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt ttcaatcgtg
    11041 acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc gtacattttt
    11101 ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt cgtacattga
    11161 ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg actggcggaa
    11221 ttggcctcgc gaagttccat catcctatcg aactattgcc ggttatcggt acgattgtca
    11281 gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa
    11341 cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcacg atgattgagg
    11401 gtagttttct gcttatgaat ggcctgaata tcattcgttt ctggcggatg cagaaaaggg
    11461 gaattgatcc gtttaaagta gagaaaaccc ctcccgccat agacgaaagg ggttaaacaa
    11521 ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt
    11581 gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca atacgccgat
    11641 aataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga acatattaca
    11701 ggccagtggg atcagcagca aagaaccccc cgccacgccg gatgcgccac aggcacacag
    11761 agaagccact acgctcaaca gcagcgctgt gggcagatcg accggaatac ccagcgtatt
    11821 aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat tgatggtggc
    11881 tcccagcgga atagaaacgg aataggtatc gcgatccaga ttcagctttt cacacagtgc
    11941 catattcacc ggaatgttcg ccgcagagct gcgggtgaag aaggcataca cgccgctttc
    12001 gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc acaccagcaa
    12061 tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca gcagttgcgc
    12121 gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca gaccaaaaat
    12181 accaatcggt gcgaagcgaa tgaccagttt caccataaag gtgacggcat tcgacatatc
    12241 gttgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc
    12301 ccacaccagg atcccgatgt agttaccttt cagcagcgcg tcgatggggt tggaaaccat
    12361 gctcattacc agcccgcgca tcacttctac aatgcctgac ggtggcgaaa tatcacccgc
    12421 gctactggat aagtgcaggg tagaagggaa ggcgaagctg aaaacgactg cggccagagc
    12481 agcagagaag gtacccagca gatagaggaa caaaataggg cggatattgg ttttctgccc
    12541 gtgctggtgg ttagcaatag atgccatcac cagcatcaac accaggatgg gggcaacggc
    12601 tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg cgggttttga
    12661 gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta ccaggctgcc
    12721 atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc gatcctttca
    12781 ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt
    12841 gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat gctggatttt
    12901 aaagagaggt tatattttgc aacttaatgg ttacatttat ttgacataaa aataaagcca
    12961 acgtgcggct tccagactga ggactacgtc aaaaaaggac tcggtcagtc ccctcgcccc
    13021 gtttggggag agggttaggg tgaggggaac aggccagcat cggtgcgaaa aattaccctc
    13081 accccgaccc tctccctgga agggagaggg agaaaacggt gctcatgttg atgacagggt
    13141 acacggctta ggcagattaa aaatcatcca acccgctgct tatcatgccg ataattcacc
    13201 caggcgttga taataaacgt catcaccagg atgccaaaca ccacgcccag cgagacggcg
    13261 attggaatat ggtagaagtc gacaatcagc atcttgatac cgataaacac cagaatcacc
    13321 gccaggccat atttgagcat cgagaaacgc tctgctacgc ccgccagcag gaaatacatc
    13381 gcacgcaggc cgaggatcgc aaacaggttt gaggtcagca caatgaacgg gtcagtggtc
    13441 acggcgaaga tagccggaat gctatccacg gcaaaaatca cgtcgctcaa ttccaccaga
    13501 atcagtacca gcatcagcgg tgtggcgtac agcaatccat tcttacgcac aaagaaatgc
    13561 tcgttgtcga tggtgtcggt catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg
    13621 tcgccaatgc ctgattcatc ttcatgggcg agcgccatct tcacgccggt aaacagcagg
    13681 aaggcaccga agatatacag gatccagtcg aactgcgaaa tcagccagct gccagtgaag
    13741 atcatgatgg tacgcagaac aatcgccccg agcacgccat acaccagcac gcggcgttgt
    13801 aatgccgccg gaacggagaa atagctgaac aacatcagcc agacaaagac gttatcgacc
    13861 gccagcgact tctcaatcag ataacccgtg aggaaggcca gcgcctgtgg atcggcaacg
    13921 gcgcgacctt cggtttgtac cagataccac cagaaagcag cgttaaacag taacgacagc
    13981 gtcacccaga ccagcgacca ggccgcagcc tgtttcatgg tcatggcatg tgccccacga
    14041 cgtccctgca acaacaggtc gatagccagc ataatggcga caacaacagc gaatccgccc
    14101 catagcaacg gcgtgccgac agtattcata gaagttcctt acacataaaa aaaacggcca
    14161 acgtcggaag acgtcagccg ctgcttttta tgcatagacc tcgccttccg gcaaggtctc
    14221 acttacaaca aaaaaggaat acgtctgcgt attcctttcg ggttgcccgg tgaccggatg
    14281 tggtttttca cacatcgtaa tgacgatcga ccggcaatga agttactccc ctttgcaggt
    14341 aacaaagtat gacagaccat tcagtcagtc aatggtctgt tacatacctt ttacactgtt
    14401 ttactttggc aattatagtt ttacgctatc tgccggaaaa atcaccccag tctggcggcg
    14461 gatctcggtc agcagtttgg ctgtgatgcg actgaccgcc agccctggat gatccaccag
    14521 atgctcatcc accagggtag cgaacagctc tgcttcgtag agcatggtat taatatgctg
    14581 cggctgggtg agatcctgca tctggctgcc acgcggcacg aagcacactt tctggcattc
    14641 agacagtttt tcaatcacca gcgatcccgc ttcgccctga atctcgcttg ccaggacaga
    14701 atcgctgacc tttgagtgct gcaaggtgac gctgaaatca ccgtaatcca tcaccaccac
    14761 gccgtgggca tctacaccac ttgccagtaa actggcggtt gcctgcacgc ttttcggctc
    14821 accaaataac gctaccgccg acgccaggca gtaaaagccg atatccataa ttgaaccgtt
    14881 agagaatgcc ggattaaagg tgttgggatt ctcaccgtcc aggtaacgtt gataccgcga
    14941 ggaatactgg caatagttga aaaaaacttt ccgcagtttg ccgactttcg gcagcgcctg
    15001 gcgtaacaga tgaaagttag gcaggctggc cgttttaaat gcctcaaaca gcaccacctg
    15061 attttcacgc gcgcaggcaa tggctgcatc cacttccgcc agattcgacg ccagtggttt
    15121 ctcacaaatc acatgaattt tgtggctcag gaaaagttgc gtctgggaaa aatgcagggc
    15181 gttcgggctg gcaatataca ccgcgtcgat agcgtcactt tccgccattg cttccagcga
    15241 agtaaacaga tgctcgacag aaaactcatt ggcgaagtgc tgggcctgtt caaggctgcg
    15301 ggaataaacg gcggttaact tatatttacc gctttcatgt gcggcctcca cgaactggtg
    15361 agttatccag ttcgtaccaa tcacggcgaa acgtatcata acgcgtggat actccataaa
    15421 gggattctgt cgtcaatgta gcacgccttt ttcatcttcg acgtgcgcgg atacgcatta
    15481 tttctgcgcc ggacaaccat gctcactaaa cggaccaata atgaccgacg gcccgagata
    15541 ttttggctga cgattgaaga ttttcgcgtc aatcaccgcg ctgactctgg cgagtttctc
    15601 tcgcagacgg acaatctttc cgcgtccttt ttccaggtct ggtgcgttaa acccgatagc
    15661 atcgatgccg tattgttttg ccagccagat ggcgcgttgg ttatgaaatt cctgcgagat
    15721 aatggtgata tggttctccc caaacacttt attggcacgc accactgaat caagcgttga
    15781 gaatccggca tagtcacaga agatcacttt tgccggtacg ccctttgcga tcaaagcctg
    15841 ctgcatgcct gacgcttcat cgtaattttt acgtccgtta tcgccactga ccagcagcca
    15901 tttcactttt ccggcgtggt acaactctgc tgcggtatca atacgtcggg taaagtagcg
    15961 attacccggc ctggccccca gcaataagcc cacgttacgc gccggaacga cgttgacgtc
    16021 gctccaggtc agctgtttgc tggcgttgac cattacgcga tcggcgataa aaatggtcgc
    16081 tcctgccaca agtaacagta agcaggcaat ctttagagtc cggcgagaaa aacaaatacg
    16141 gagaagaagg cgggcaaatg cgcgcagcat ggtcgttatc cttaacgaaa ggggggaggt
    16201 cgattttagg cgctaaagaa agaaaagata acgcctgaca tcattttcag attttgtcct
    16261 gcccaagcga caactgggtt agccacagtc gggtgtcgaa ctcaagctgg tggtactggg
    16321 gttccatatg acagcacaac tggtaaaacg ctttgttatg ctctttctcc ttcaggtgcg
    16381 ccagctcgtg caccacgatc atgcgcaaaa acggttccgg cgcgttgcga aacacggtag
    16441 cgacgcggat ctctgctttt gctttcagct tgccgccctg cacacgagaa acagcggtat
    16501 gtaacccgag cgcgtttttt agcacatgga tcttattgtc atacatcact ttattgatcg
    16561 gcggggcatt acgcagaaac tgatttttca gatcctgagt atattgccag agggctttat
    16621 cggtggcgta gtcgtgtgtt cccggatagc gttttgccag cacatcgccc agacgctgtt
    16681 cgttaatcag tgttcgcacc tgggaaagta gctgctcggg ataaccctga agataagtaa
    16741 gattgctcat aaccgcccac gaaaaagaga aaaagggtat actcacgcac ccttttcagg
    16801 ggcaatgtcg aaattttatc attcaggagg gccgatgagc cacttagaca acggtttccg
    16861 ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt aacccgctac aggcgtggga
    16921 agcggcggat gaatatttgc tgcaacagtt ggacgacaca gaaatccgcg gcccggtgtt
    16981 gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg gcggaacata agccgtacag
    17041 cattggcgac tcatacatca gtgaactggc gacgcgcgag aatttacgcc tcaacgggat
    17101 tgatgagtcg agcgtgaagt ttctcgacag caccgccgac tacccgcaac agccgggtgt
    17161 ggtgctgatc aaagtgccga aaacactggc attgctggaa cagcaactgc gtgcgctgcg
    17221 caaagtggtc acgccacaaa cacgtattat tgccggtgcc aaagcccgtg acatccacac
    17281 ttccacgctg gaactattcg aaaaagtgct cggcccgacc accaccacgc tggcatggaa
    17341 gaaagcgcgc ctgattaact gcactttcaa tgaaccaccg ctggccgatg cgccgcagac
    17401 cgttagctgg aagctggaag gtactgactg gactatccac aaccatgcga atgtcttctc
    17461 ccgcaccggg ctggatatcg gcgcgcgctt ctttatgcaa catctgccag agaatctcga
    17521 aggggagatt gtcgatctcg gttgcggtaa tggcgttatt ggtctgacgc tgcttgataa
    17581 aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg atggcggttg cttccagccg
    17641 tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc tgcgaattta tgatcaacaa
    17701 cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg ctctgcaacc cgccgtttca
    17761 tcagcaacat gcgctgaccg ataacgtcgc ctgggagatg ttccaccacg cccgccgctg
    17821 cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgt cacctggatt acttccataa
    17881 actgaagaaa attttcggca actgcaccac catcgccacg aataataaat ttgtggtgct
    17941 gaaagcagtg aagctggggc gtcgtcggta agattgacag ctacctgcca gataaggcgt
    18001 aaacgcctta tctggcatac gtttagcaca ttacccccag gtcggataag atgcggcaag
    18061 cgtcgcatcc ggcaatgtct gcccagttct ggaatcgcgc tccataaaac aaaaaatccc
    18121 tgtcaccatc ctctacattc tcttgtttag cgttgtttaa cgtttattct tccgtcacac
    18181 agataaattc atccgttgcc aatctgtcaa cggatgttat catattgcca atttgtcaac
    18241 ggacgtgatg aagaatgcac ctgataactc aaaaagcatt gaaagatgct gcggaaaaat
    18301 acccgcaaca taaaacggag ttggtggctc tagggaacac gattgctaag ggatatttca
    18361 aaaaacctga gtcattaaaa gcagtattcc catctctgga taacttcaaa tatctggata
    18421 agcattatgt tttcaatgtt gggggcaatg aattacgtgt tgtagcaatg gtcttttttg
    18481 aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa agaatacgat ttttttaccg
    18541 ctgttcatcg tactaagggg aaaaaatgat tgcgattgcc gacatcttgc atgcaggaga
    18601 aaagctaact gctgtggcac cttttctggc gggtattcag aacgaggaac aatacatcca
    18661 ggcgctggaa ctggtagatc atctgctgct caacgatcct gaaaacccct tactggatct
    18721 ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc gaatttgcgg aatttaatgc
    18781 catggctcaa gccatgcctg gcggtatagc cgtgattcgt acccttatgg atcaatatgg
    18841 tttaaccctt tccgatctgc cggaaattgg cagtaaatct atggtgtcac gcgttttgag
    18901 cgggaagagg aaattaacgc tggaacacgc taaaaaattg gcaacgcgat tcggcatttc
    18961 tcccgccttg tttattgatt aacgcg
//
LOCUS       DAZFTN010000086        18858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFTN010000086 DAZFTN010000000
VERSION     DAZFTN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..108)
                     /locus_tag="ACHKV2_003473"
     CDS             complement(<1..108)
                     /locus_tag="ACHKV2_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941297.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEEN"
     gene            complement(254..4156)
                     /gene="hrpA"
                     /locus_tag="ACHKV2_003474"
     CDS             complement(254..4156)
                     /gene="hrpA"
                     /locus_tag="ACHKV2_003474"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO6941298.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4357..4962
                     /gene="azoR"
                     /locus_tag="ACHKV2_003475"
     CDS             4357..4962
                     /gene="azoR"
                     /locus_tag="ACHKV2_003475"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO6941299.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5013..6305)
                     /gene="ynbD"
                     /locus_tag="ACHKV2_003476"
     CDS             complement(5013..6305)
                     /gene="ynbD"
                     /locus_tag="ACHKV2_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO6941300.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6319..8076)
                     /gene="ynbC"
                     /locus_tag="ACHKV2_003477"
     CDS             complement(6319..8076)
                     /gene="ynbC"
                     /locus_tag="ACHKV2_003477"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO6941301.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8092..8988)
                     /gene="ynbB"
                     /locus_tag="ACHKV2_003478"
     CDS             complement(8092..8988)
                     /gene="ynbB"
                     /locus_tag="ACHKV2_003478"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6941302.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8988..9593)
                     /gene="ynbA"
                     /locus_tag="ACHKV2_003479"
     CDS             complement(8988..9593)
                     /gene="ynbA"
                     /locus_tag="ACHKV2_003479"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6941303.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9764..12070)
                     /gene="ydbD"
                     /locus_tag="ACHKV2_003480"
     CDS             complement(9764..12070)
                     /gene="ydbD"
                     /locus_tag="ACHKV2_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO6941304.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12134..12994)
                     /gene="pdxI"
                     /locus_tag="ACHKV2_003481"
     CDS             complement(12134..12994)
                     /gene="pdxI"
                     /locus_tag="ACHKV2_003481"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO6941305.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13214..15673)
                     /locus_tag="ACHKV2_003482"
     CDS             complement(13214..15673)
                     /locus_tag="ACHKV2_003482"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6941306.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15893..18715)
                     /locus_tag="ACHKV2_003483"
     CDS             complement(15893..18715)
                     /locus_tag="ACHKV2_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO6941307.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVKHNPVVYKNG
                     VTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSWTVHDDSNTVVFDGVNT
                     SGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQGGNTALNGGTVMTVDG
                     DNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGTAVIINGDNTKLNTAGD
                     STITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGATIKQEGDLYVSGGAHG
                     IDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLNVGQAAAGENAIGIQID
                     GDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFSTGLDVSGNNNNMTLAT
                     YELNVTGQKATGVNVSGDSKTTSRSYASYDGAQEHIAGIVIAGDDNTFTLNGGVHFVG
                     EKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGFENLIQLSHGA
                     ELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNIGFAAAWNKDS
                     TVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSVLNYPAEWNLS
                     DGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKSTADNEGQITLD
                     TLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHENGVITVYNAG
                     AGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAINDQTGVININA
                     ENGQAFYNDGTGLIINYGTICTFGVC"
BASE COUNT         4769 a         5062 c         4255 g         4772 t
ORIGIN      
        1 attttcctct ggaagttttt catagaaaga tattaaagcc tgaccaacgc gatcaccctc
       61 tattatccgt tcaaattgcg acctcagtaa aatatcatgt ctactcattg caatacgtcc
      121 tgttcgccca aggcgaagta tgaatgaaac ttatgtaaaa tagtaccgcc cttgctatat
      181 ttcagaattc ataaagaaaa aacccggcgc taaatgctta ccgggtttta gatttatcag
      241 gcaaatagca gggttaaccg ctaatctgct ccatcgcctg caaaatacgc ttatctgaaa
      301 tcggataagg cgtaccaagt tgttgagcga agtaactaac gcgcagctct tctatcatcc
      361 aacggatctc tttcacgtct tcatcctcac gacgtgcagg cgggagtttg ttgaaccatt
      421 gctgccacgc ctgctggacg ttttcgactt tcagcatctg tgcacgatcg cgatgtggat
      481 caaccgccag tttttccagc cgtttttcaa tcgcctgcaa ataacgcagc gtgtcgccca
      541 gccgtttgaa gccgttacca gtgacaaaac cgcgatatac caacccgccc atctgcgctt
      601 taatgtcaga aagccccagc gccatggtca tatccacccg ccctttcaga cgtttgttga
      661 tattgaacac tgccgtaagg atttgctcga cctgcttcgc aatatccacc accgtgtcgt
      721 tcagttcggc acgcactttt tcatgcagcg cagcaaagcc ttcttccgtc cagaccgggc
      781 ctccattggc gtcgatcagt tgatccacac cgcatgagat acagtcgtcg atcagctcca
      841 gcactttgcc atacgggtta aagtacagcc ccagcttggc tttgtttggt aacttctcat
      901 gtaaatactt gattggtgag ggaatattca gcagcagtag acggcgaaga ccgttccaca
      961 ttgcctgttt ttgctccagc gggttatcaa acagtttgat cgccacactg tcgcgctcat
     1021 ccaccagcgc cggccacgcc ttcactttgt agttaccacg cttctgttcg tagctttccg
     1081 gcaactgacc aaaactccag atatgtaagc cgctctgctc gataccgtca tccgccaccg
     1141 cagatagcgt ttcctgcact ttgcctttca gcgcatcttt cagatcttgt agcgagcgcc
     1201 cttcttttag cttcttgttt ttgtcatcca ccacgcggaa ggtgattttc aggtgatcgg
     1261 gcacctgatc ccagtgccag tcttcgcggt caacggtaac gccggtcatc cgccgtaact
     1321 cgcgctcaag gctgtcgagc aacggtaact ccagcggtgt gacgcggcct aaaaacgctt
     1381 cggcatagtt tggcgcaggt acaaaattac ggcgtaccgg tttcggcaac gatttaatca
     1441 gagcaatcac cagttcgcgg cgcagaccag ggatctgcca ttcaaacccg ctttcctcaa
     1501 cctggttaag taacggcagc ggaatatgta cggtcacacc gtcagcatcc gcgccgggct
     1561 caaactgata gctcaaacgc agcttgagat tgccctgatg ccagaagttc gggtaatcca
     1621 gcttgctgat cttttccgcc ccttctttga tcaacatgct cttttcaaag ttgagcaaat
     1681 caggcgtttc gcggctgact tttttccacc agctgtcgaa gtgacgagcg gagattacat
     1741 cgtggccgat gcgctggtcg tagaactcaa acagcgtttc gtcatcaacc agaatatcgc
     1801 ggcgacgtga tttgtgttca agctcttcca cttcggcccg cagtttcagg ttttcacgga
     1861 agaatgcgtg acgcgtctgc cagtcacctt ccaccagcgc gtggcgaata aagagttcac
     1921 gacataacgc cggatcgatc tggctgtagt tgaccttgcg cgcggcaaca atcggcaaac
     1981 cataaacagt gactttttcc gttgccatca ccgcgccctg cgcccgttcc cagtgcggtt
     2041 cgctgtaggt acgtttaatc aaatgctgag cgacaggctc cacccattcc gggtcgatac
     2101 gcgcagcaat gcgcccccac aggcggctgg tttctaccag ttccgccacc attacccatt
     2161 tcggcggttt tttgaataaa ccagaaccgg ggaagatgga gaaacgcgcg ttacgtgcgc
     2221 cggtatattc ttgtttatcg gcatctttca tgccgatatg ggaaagcaac cccgtcagca
     2281 acgcaatgtg aatttcacga tactccgccg gttcgctgtt caccggaatg ccgagttctt
     2341 tcaccacctg gcgcaactgg gtgtagatat cctgccattc gcgcacacgc agatagttga
     2401 gataatcggt acgacacagg cgacggaagg cgttggaaga aagcgccttt tgctgctcgc
     2461 caagataatt ccacagattc acaaacgcga ggaagtcaga ctctttgtcg tggaagcgac
     2521 gatgtttttc gtccgatgcc tgctgtttgt ccatcggacg ttcgcgcgga tcctgaatgg
     2581 agagcgcaga cgtgataatc atcgcctcac gcacgcagcc atgtttttgc gcttccagca
     2641 ccatacgcgc cagacgtggg tcgacaggca actgcgagag ctggcgaccg agcggcgtca
     2701 gtttataggc gctggcctgt tcatccgtgg tgatcgcgcc cagttcttcg agcagacgca
     2761 cgccatcctg gatattgcgt ttatccggtg cttcgacaaa cgggaacgca gcgatatcgc
     2821 ctagccccag cgcggtcatc tgcaaaataa ccgaggccag gttggtacgc agaatctccg
     2881 gatcggtaaa ctccgggcgc gagaggaaat cgtcttcgga atagagacga atacagatcc
     2941 cttcggacac acgaccacag cggcctttac gctgattggc agacgcctgc gaaatgggct
     3001 caatcggcaa acgctgcact ttggtgcgat agctgtagcg gctgatacgc gccgtaccgg
     3061 ggtcgataac gtatttaatc cccggtacag tcagcgaggt ttccgcgacg ttggtcgcca
     3121 gcacaatgcg tcgcccgctg tgcgactgga acacgcggtt ctgttcgcta ttcgaaagcc
     3181 gcgcataaag cggcaagatt tcggtatggc gtaagttcag cttgttcagc gcatcggcgg
     3241 tatcgcgaat ttcccgctcg ccgctcataa agatcagaat gtcgccgggg ctttcctggc
     3301 ttagttcgtc cacggcatcg aaaattgcct gcaactggtc gcgctcagtg tcgtcggctt
     3361 cttcaacaat cgggcgatag cgcacttcca ccggataggt ccgaccggag acttcaataa
     3421 tcggcgcatt attaaagtgg cgcgaaaagc gttccgggtc gatagtcgcg gaagtgataa
     3481 tgatttttag gtcaggacgc cgcggcagca actctttcaa ataaccgagc agaaaatcga
     3541 tattcaggct acgttcgtgc gcttcgtcaa taatgatagt gtcgtactgc atcagcaggc
     3601 ggtcttgctg gatctccgcc agcaggatac cgtcggtcat tagcttgacc atcgtgttat
     3661 cacttacgtg atcgctgaaa cgcactttgt aaccgatgca accgcccggc tccgttttca
     3721 gctcttccgc aatacggttc gctaccgttc ttgccgccag gcgacgcggc tgggtatggc
     3781 cgatcagccc tttaatcccg cgccccagct ccatacagat tttcggtaac tgagtcgttt
     3841 taccagaacc cgtttccccg gcgactatca ccacctggtg atcacgaatc gcttcgagaa
     3901 tgtcctgttt tttctgacta accggcaaat tgtcaggata agtaatttcc ggtcgtgccg
     3961 cttcacgcag caggactttc cctgccgcct ggtcaatctc tttcgccatc tcctggaaaa
     4021 tggcctgttg tgcatcagga tttttaacct tcttcacgcc gtgcagacgg cgagaaaaac
     4081 gcagtctgtc acgcagcatc agcgaatcca gccgctgctg caaggccgta aaagtcaatt
     4141 tttgttgttc tgtcataacg ttagtgggca gtagtctgcc tgattaattt tctttgaaat
     4201 gatagatata gagtaccaca tcgcggcttt ctatgcgttg ttcaataaat tcgaacatag
     4261 gcttcgattt attgcgctat ctctgtggca ggattgtgaa taaagtgtca acaagcaacg
     4321 gggcatcgcc cattcaaata tctataagga aacaccatga gcaaggtatt agttcttaaa
     4381 tccagcatcc tggcagggta ctctcagtct aatcagttgt ccgattattt tgttgaacaa
     4441 tggcgcgaaa agcactccgc tgatgaaatc accgttcgcg acctggctgc aaatccaatt
     4501 ccggtactgg atggcgaact ggttggcgct ctgcgtccga gcgatgcgcc gctgactacg
     4561 cgtcagcagg aagctctggc actttccgat gagctgattg ccgagctgaa agcccatgac
     4621 gttatcgtta ttgcggcacc gatgtataac ttcaacatct cgactcagct gaaaaactat
     4681 tttgacctgg ttgcacgtgc aggcgttact ttccgctata ccgagaatgg tccggaaggt
     4741 ctggtaacgg gtaaaaaagc catcgttatt accagccgcg gtgggatcca caaagatgga
     4801 ccaacggacc tggtgacgcc gtatctgtcc acgttcctgg gctttatcgg cattaccgat
     4861 gtgaaatttg tcttcgccga agggatcgca tacggtccgg aaatggcagc gaaagcgcag
     4921 tctgacgcga aagcagccat cgacagtatt gttgctgcat aaccggacac gatcttgatg
     4981 tggactgtag ttcagtccac atctcaatcc acttacctgt tttcccataa tttcaacatc
     5041 gctttgtgct cttccttaag cacaatatgc gagcgtctgg ctcgaataaa gctaatcgct
     5101 tcatcaactg ttttacagtg tccgtaacat aacagccatg ccgccactac cagcgcactg
     5161 cgcgataatc ccaatgcgca atggaccaga acgctgcctt gctcttcgcg taatgtttcc
     5221 agcatcgcca cggcctgtcg gagctccccc tcttccggaa ccaccagatc cagcatcggt
     5281 acacagaaat agagtcgatc ttttgtcgct cgtccgcgag ggaattcaaa ggtgacatcc
     5341 agaaccgcat tctgtgccgg aatatgtcgt ggaaacgccc ctaaataaac accagcagtc
     5401 attttgctca ccggctccag gcgacgacaa aaccaacgca tagatagcca catcccgatg
     5461 cgccagggca atgtcagcca gtaaacggcg ggcgggagtt tcccctgact atctttccct
     5521 gttgttatcg cgccaagccc accgtaccca cggccaataa tgagtagcga taatactggc
     5581 caacataacc agactgacca ccataactga accatcatca ttagctccat caacacaatg
     5641 cacgcgcacg cgcctacgac atatggcagt gctattttga ttcgacgttg atcaggtttc
     5701 tgataattcc aacgacggtc gacgggtatc atccagtcaa tcaacatacc taccgccagc
     5761 cctgtgatga catcaataaa atgatgctgc caggttgtca gcatcgaaat ggcgatgagt
     5821 aaaaaccatc cgccgcagac tttacgccac ctcacagcca gatgctgacg aaagtgacgc
     5881 caaagtagcc agcagagaac aatatgcagc gaaggagact ggttataagg cagatcaaac
     5941 agttcaagtt gcgaaaatag ccatcctgtc accccactca cttcaggacg gataaaacta
     6001 aacttcagcg ggtagagaaa aaaaccgcag caggccatta ccgttgccag aataagccgg
     6061 tggacaagtc ggcgctgttc gaatgtcgag ctacaaacga acagcgaaaa tccatataaa
     6121 agatccagac tccagtaagg gacaatagtc cagggaagaa aagggatcgc cgtttcccag
     6181 ccgaatacct gactggggat atcatggctg ttaaagtcct gaaccgcggt gaactgattt
     6241 agagatccat aggtgaagaa gaaaaacggg gccagcaaca ataaccagcc agcgccttgt
     6301 agcaatacgt tccggcgttc agttatcacg acgcaccgcc atcgaaaccg taaaaatgcc
     6361 ccactcatca atccgttgtg tgcatttatc aaatccggca tcacgcacga gtgaatccat
     6421 ctccccttgc gaacgcacgc gcattaccca cggtttacca tctttatgac tggttaacac
     6481 cccggcaatc agctccagtt gtgggtgcca cggctgcccg gtgtagagga gaatgccgcc
     6541 cggttcgatg gcattggcaa gacctgcgag tgagtttttt acctgctcat ttccgggaaa
     6601 aagctcatac agtccagaga caatcgccag cgtaggccgc ggagttaacg cgctgagttc
     6661 ctccgggtta aacgcatcgc cctgttcaaa acgcacccgc ccagacattc cccgttgagc
     6721 aatcatctct tgcccctgtg caacatttaa ctcgctgtaa tcacgtaaca aaatatcgct
     6781 tacggcaggc tcgtttgcca gcgcatccag tacatagcgc ccatgccctg cggcaatgtc
     6841 aaccacgcgg acggctaaac ctttggcgtg gagatcggca acggcttgtt taatcagtat
     6901 ttgtaaatgg gttttgcgct ggcgaatacc gcgccagcca acactgttca ggtagatttt
     6961 gtcgattaag cgcccgaatg cgttactacc ttgcggttga ttttgataga cataatccag
     7021 cgaactgcct gaatcaaagc cggtgctcat tccgagatgc aggcccgcag agtgcgtccc
     7081 gaacaatttc atcgctttac gcataaagcg atacgccaaa tcaaccggcg ataatggcac
     7141 gggtccacct gaaaggagcc gccagcgatc cgctgatggt ccagtgcggt cttcatgctg
     7201 ataatcaaat ttttgtgact tgttagcata taaacgacta ataaagcttt gcattttttc
     7261 aaatgcctgc gccctgttct cttcacccaa cgtgtcgtga taaaagcctg gcagcagatg
     7321 cagctctttc agagggctac gtaatctatg ataaaaatca atctgcggtt ggcgatgcac
     7381 cacatagtca tcgcctgata tcagaagttg cgtggggagc gtaatcgccg ccgcatcgct
     7441 aacaatacgt tcagacgttt tataaagatc gagcaagata ttaacggcaa tcgcccgtgt
     7501 gatcagcgga tcattattga aactcgccac ccgttgccga tcgtgggtca aatagcgtcc
     7561 tttcacatag gaattaataa aaaacagacc acgcagacga tgccataacg ccagcgcagg
     7621 acgtgccagc ggcacataca atttaacctt aaaggccgga gaagccagca ccagcccgcg
     7681 aattgcaggc gcataatcat gaacccatgt ggcaaccagc actgcgccaa cgctctgcgc
     7741 gatcactacc acctcttcca gcccgacctg gctgtcgctg gcagcaaaac ggacaaactc
     7801 atcgacatcc cgcactgaac gcgcaagaga tgggctataa ccacggggcc ccgaagtttg
     7861 tccatgccct cgggcatccc atgcataaaa agcagtatct ggcatcgcca gttcatcaac
     7921 gatatgttgt agacgaccag aatgttcatg accgcgatga aataagacga tgaccttttt
     7981 cgcccctggc tgtaaagcgg gccagtgccg ataaaacaac gctgtattat cactggtggt
     8041 aaaaaaatgt tccccaggga tgcgtgaatt ttccattttc tctattttcc ttcagtaaca
     8101 gcagtagcga ataaaataaa aaaataccgg agcggtgaaa attaatgagt caatcctgtc
     8161 aaggagtccg ccatgtcctg gtaatagttt tccgctatct ttaacaccca catctcgttt
     8221 gacggctgac atcactacat cgccgcaaaa accactaata ccaattaaca aacccgctaa
     8281 taatgcctgt aatgtattta gcggtgtcag caacggtcca ataatcagtg acgcaatcat
     8341 ggtggtgatt acgcccccca ccagaccttc cagcgttttt cccgggctga ctttggggac
     8401 cacttttctt ctgccgcagg atttccccca taaatactgt gcaatatcgt ttgactctgt
     8461 taacgccaaa agaaataaca ccagtaacgc cccgccctgg gtatttgtgg ttgtcaacat
     8521 taataaccag gcggcatgac tcaaggcgaa aacggtcatt atccagcccc aaaaaatggc
     8581 gctcacggta tgcagaaaac cggaggtatc acctactaaa actcgccagg tggttaatat
     8641 cagaaaccca gccaaaggta taaataacag aaacagctca aagcaactaa aaccgataag
     8701 caaatagtta agaggaataa cccaatataa ccaacgcgga aaatgtacgg atataagcgt
     8761 acaatattct ttcaatgcca gaaaacttat cagcgcaaaa aacgtcaacg tcatccagcg
     8821 tggacctgaa atcaccagtg aaaaacaaat gacgataccc caccaggtac gaatacgtag
     8881 tgtaacttca tccccttttc tctccggtaa ccgccatacc cgaaaacggt ttatcagcgt
     8941 tgctaaaatt aacaacgcga aaagtgttgc cagagatttt tccagcatta tctttcagca
     9001 ctcatcagga gcacactccg acaacgatta atcgcagtcc agagaagcag aattgaagca
     9061 atgctccata aaagattatt ccattgcatc cactgtggat aaatggcaac ggccagcccc
     9121 cacaacccaa atattaacgc gcgatcgctt ttgccaaaag gaccgacata actacgaata
     9181 ccgttaatcg tctgcgcgag taaaccgcaa aactcagtca atatggtgca aaataacatg
     9241 agtattacga gtgatgcgtt actttccggt aaaaataaaa acggtaaata gagcgcaata
     9301 tcggaaataa catcgccagt ttcatttaaa atcgccccca gacgtgtttg ctgattgcac
     9361 tcacgcgcca acatgccatc cagcgcattg agtgccatac ggataaaaag cacgatgggc
     9421 aatagcaaaa agaggatggg ttgtgcgacc aacatcaaca gcaatccggt aataagagaa
     9481 agcgccagtg cagcaagagt gatgtgattc gctgtaacat ggtgcttata aagccaaaac
     9541 atcgtcggcc ttaacagcga ctgaaagagc ggttttatct ggtagagcgt cattatcaat
     9601 ccttgataat tcggtttgcg ctatggtgca tcaattcccc tgacggagat atcgcaacac
     9661 gacgcttcat taaaaattca gaatatatca aggtattggg gactcactgt caggaatgat
     9721 tatccccaat aaatcaagtg gttaaaaatt attacgctgt tattcaatac aactcatcaa
     9781 gttttctcat cgcttgttcc cgtattgcgc gcgtcttacc gttaagcgcc agctggcgaa
     9841 ctaaatccag ttcagggtta acaagcttta acgccagcaa aggatcacgt ttcagcctcg
     9901 cctttaatac atcaactgga aaacggggat tattcatcgc cacaatccac cactcgggat
     9961 caatttctgc cgccagtact gcaggcggtg tgtggcggtt gttaagtgcg ccgaacttga
    10021 aaaatgtgag attgcttttc gccagtgtac tccaccatat acgatccggt tgttttgcca
    10081 gtacatccag ttgtgctaac gcatcggggt ctagtgcata ataaacacgt aacgcttcgt
    10141 taacctgcgc acgggaactg ttttcctgcc tcagaaaaaa cttcgcccaa atatcattgc
    10201 gtggcgtgtt ttgctgtaat tccttgagaa caacagcggg taaattatcc caactaatta
    10261 atgcactctc taaaaagtcc cagtcgtccg aacgtatcag agaggtggca tttaatagca
    10321 tctggtcatc aacttcaccg tagctgataa gctcagacaa aatcatatca gcgctggttt
    10381 catatgcatc tgaatctgct ttgggattat ttttccgatg cgataaagca cgttgccaaa
    10441 ttttttgttt catcgacgtg ctcagatact gatttttcgc cagattactc cacccctcag
    10501 gatcgttgaa acgtgtcaat aaccgttgta aagcagcgtc agagtgaatt atctgcatat
    10561 cccgacagca aaagtaggca ggggattttt tctccatcct tttaacaaaa ttaaactgta
    10621 agttatctgg caacgctaaa aataacgcac ctattagatc atcgtcattc tcgaattttt
    10681 ggtataaata tactgctatt ttgtcactct cttgtggcgt catccgcaac aattgaccat
    10741 ggtgtttcat ctctgccagc tttaacgcta agcttgtgac aacatcgttt gatgaatcgt
    10801 tcgccaactg cattgcctga ttgtaggtga gttgtcctcc agcaccgacc tgacgaatag
    10861 aggaatcatc actgcggata tagccatcaa taatggattc gggcaataac cacgcatctt
    10921 tataaagctc ctgaatgatc atatcccaat ctcttttcgc cagagtcatg acgatatcca
    10981 gagggagatt ttcacgtagc gcaagattca acggaccgac accatcaggt agatgactca
    11041 ttcgcgtcat tatttcgaca gagagcggtt cacggtcgag caaccccaga acaacaggct
    11101 ggtaacccaa ttgctgatcg gtcaactcca gaatctcatt gtcaggcata tatctcgcca
    11161 tactttcaat cgtttgtcca tcattgctat taataagttg tttgcgtagg ttagctgaaa
    11221 ttcctttaag ccttgggaga gttcgcatta tggcaagatc aaatgtcgca atatctcgtc
    11281 cttcaaatgc agcaacgttg ccttcatcca gcatttgcaa ttcctgcact aactcaggag
    11341 aaggcttaag gcgaaacagc gcttcttttt tcatctgatt acagacattc cctgccatgc
    11401 gaatattgct tcgcttaaaa atttctttca gagcatcttc atcatttatt agaccccaat
    11461 aatatgcatc aggatttatt atcatcagcg cttttttgac attttcagtg tcatttttgt
    11521 cactcgcacg gaccatgaat tcactatttg tcgaagacgc aacaacaggt gcaattatgt
    11581 cattgataaa tatcccacgc gcattaatac tccccttaat cgtagacgga ttatcgtaat
    11641 atgagtcgat aaataccagt ccacttgcat ttatcgaacc ggtaattaac agtggtgaat
    11701 ccgtaactat attcgttgcc gtcacgttac cgagaataac ccatggatta gaaagagaga
    11761 aagtggaaat attacctttg actgtcaaat tgccaagaac aatcaatttt tgattactct
    11821 ctatattacc ttctaccact aaatcgccac gatgcacttt ccagtccgat gcagataaac
    11881 cgaaggggac aagattcacg ctactgttga tagcttgttc aacttgttgt ttatcttcag
    11941 cggtagccgc agaaaaaaaa ggttcaatat caactgcatt tgcaacgcat cgaagttccg
    12001 cctgtactgg aagactcagc ataaacaggg gaagtaaacc tatccagctg caaagcctgg
    12061 gcttaaacat atgcactcca tgccattaaa aaagaaacaa aagggaaatg catctttccc
    12121 ttttgtatat tcgttattct cgcgaaatac catccaacgt agacaacact ttctcagaaa
    12181 gatgcaactt ttcagcagcc atattctccc gtaaatgcgc aacagaagac gtccctggga
    12241 tcagcaaaat attcggtgaa cgttgtaaca gccacgccag cgccacctgc attggtgttg
    12301 cacccaagct cgcagcaaca tcggaaagcg tggaagattg cagcggcgta aagcccccga
    12361 gcggaaagaa cggcacgtag gcaatgccgt cacgagccaa agcttcaatc attgcgtcat
    12421 cagcacgatg agcaatgttg tattcgttct gcacacagac gatttcggca atcttgcgcg
    12481 cctctgcaac ctgcgtcggt gtgacgttgc ttaggccaat atgttttacc aggccttgtt
    12541 gttgcatctc tgccagcacg gtcaggctgg cttcaatcga tccttccgcc gggccatgac
    12601 catcccccat catcacgcgc aggttaacca catccagtac gtccaggccg agattacgta
    12661 gattatcgtg caccgctttt tgcagctctg ccggagaaaa tgcgggcaac caggatgcgt
    12721 cctctccacg ccgcgcacca attttagtga caattgtcag atcgtcagag taaggataaa
    12781 gcgcttcgcg gataatctga ttggtgacgt gcggaccata aaagtcgctg gtatcaatgt
    12841 gattgacgcc caatgccagc gcctcacgca gcacggttat agcgacgtga cgatctcttg
    12901 gggggccaaa aaccccagga cctgccagtt gcatcgcgcc ataaccaaga cggttaacgg
    12961 attttgtacc gagagtaaat gtattgctgc tcatcgcacc ctgccctgta ataaaacgga
    13021 ttaagaaaaa tcagtataga aacgaaaaga gataaataat gcgaaggcgt aaacaagatt
    13081 tctggatgag tagcactatt gtcctgaaag acaccaaaac attagacagc aaaagataag
    13141 tcaagcgtcg catccggcaa taaaaaaccg caacctgtat cagcgttgcg gtttgtgtat
    13201 cacatgctaa aaattagaac gttttcttaa agttcagcat cacgcctttg tcgctgatgc
    13261 cgtcctcttt ccagtgatac gcatccagat tcagcgaact ttctttgcta ctgtacttca
    13321 cgccgacgct cgccaggctg ttgataccgc cgcccttctg accgtcatcg acgttaaagt
    13381 gctggctgcc tgcccctgca aggcttgccg tgcgctggct cttgctgtag ctcaggttcg
    13441 gcccgccttc cagcgtcgca ttcgcgctcc agccgccttt ccctgcgtag tccagtttca
    13501 gcccgacgat gctgtccacc gccgtttcgc tgccgctgtt catgctcagg ttaaagtctc
    13561 cggcattgcg ctcctgatag ccgccttcca gcgagtgacg cagtttcact ccggcgtacg
    13621 gggtgatttt cagcccttcg cgcggctcaa aggttttcgc cccttcgcta cgcaactcca
    13681 ggtactgctg tttcacatcc gtatccgccg ttttgctgac gtcgccgtaa gcgaccgagc
    13741 ggctgctgtc gagattatgc acgtcataac gcagcgcgtt gttccaacgc ataccattgt
    13801 cgaaggacat ctggtgcttc aggccaaaga actggctgta gccgccggtt acgccattgt
    13861 cgcccgcttt ctgcgcaccg tcgccatcca gacgcgcgat accgtattcc aggctcatgc
    13921 tctggctttc gctcaggtca acggttttac gcagtgccag catgtcatac tcggtgttat
    13981 ttccgagttc cgcacgcggg tcacctttcg ccaccacgtt aaaggccagg ccattgccca
    14041 ccttcggcgc ggcatccgcc agcatgctga agcggttgct taacacacgc gcttcacgga
    14101 ataccgtggt cgcctggcta ccgctcacct gcttcagggc gctattcagt tcagcagtgg
    14161 tgcccacgtt caggctggta tacagctcgt tattggtgta acccgcatcc agtgccttcg
    14221 ccacatcgtt caccgaagca tcggtcgcca catcggtgta ggcgtttttg ctcatgatga
    14281 cgtcaacgtt gccgctggca tcggtgctgc ctttggcggt ccataccacg gacgttgagg
    14341 tgatggcatc ggcgtcggtc aggttgatgc cttccaccac gttgtcgaaa ctgaccgtgg
    14401 tgtctgccgt accggcggcg aaaccggtgt tgatcccgac accattcatg ctggcgttgt
    14461 tgaccttcag tttaccggcg ctgccgtcaa cgttggtacc gacgatatag ccgttgaggt
    14521 tgttactgct gccatcgcta ctaccgctat cagtcgtttc actgaaaggt gaagtggtgt
    14581 aagtgtttgg catgtccggc aacgtgtagt cggtgtaatg cacttcggta ttgttaccgc
    14641 tgttgccgtt caccccttcc acattcacgc tgtcgccctg cttaatcaac cccgaaccag
    14701 tcacgccgtc ggcaatcacc accgtaccgt tgttaacgat atgaccttct gtgcccagta
    14761 cgctgaacgc aaacgagtcg ttagcaaaaa tattgatcgt gccgttgttc tcaattaccg
    14821 catcggcggt ggcattggca tccagttgca taccagtcaa tccggtatcg gtggtgcctt
    14881 cggtaccgag attgataacg ccgttgttaa ccagctgtgc gtttttatcg ccttccatcg
    14941 ccacggcgtt gttgccgctg atgttcatta caccatcgtt gagagcaaca tagttgttgt
    15001 gggtgaattt aaccgcgctg gcgttgtctt tatcgaagtt aattaccccg ccatcctggt
    15061 tccacaagaa ggcatcgctt tctgtcgcac cggcagtact taccgccacg gtgactttcg
    15121 catccaccac gcccttgtta ataaactcgc tggcgccatc gtcgtagtga atagcgcccg
    15181 cacctaatgc ggcatccagc gtcatttcac cggtgttata taacgtggcg ttagcattgc
    15241 tgccctggaa gcgaatggcg tttttgctgt tatcaaggat gagtttgccg ctgttgttga
    15301 acgttgactt atcgtcaata tccagcgtcc cggcattgtt gttttcatcc tgataaccca
    15361 ttttccaggt acccgcgtta ttcactaccg cagagttata gagaatcgcg cctttacgcg
    15421 cctcgccgcc tgcaaggtta ttgaataccg catcgccctg agcgttaacg cgcgccgacg
    15481 caataccgct gttattcacc gtaccatcgt gaagttgtaa agttgacagg gaaccggtgt
    15541 tattaattac cgcgttgctg tttttctcaa tttttacgac gtcattaagc gttccgttgt
    15601 tattgaaggt tcctccttcc agagtcaccg tgttcaatac accttcattg gttaacgcac
    15661 cgccttcatt cacgacgatg gctgagctaa tggttccgct cgactcgtta accagcgcac
    15721 cattttcgag tatcctgatc gttccatttt taacagcacc cgcactggtg acatccccgt
    15781 tgataatcgc acctgacgat aataatacgg tctcacccgc agaacttaat acagcgccat
    15841 catcatataa atcagcaata taattatccg ttggattata ttcatcggcc ccctagcaaa
    15901 cgccaaaggt acaaattgtg ccatagttga taataagccc agtgccatcg ttatagaatg
    15961 cctgaccatt ttccgcgtta atgttgatta cgcctgtctg gtcattaatg gcggtgcctt
    16021 cgtgataaac cgccatgccg acgagtttat tcacaccgag agaatcgttg taattttcat
    16081 ttttctccag gttgatggtt ccctggttaa tgaccgtatt agtattccca tacgccgcca
    16141 tccccgcacc ggcgttgtag acagtaatca caccattctc gtggttggtt gccgtggctt
    16201 tagttaatgc cccattatag gcatcggtac ctgaagccat tcccacaccg aaatctttcg
    16261 ccgtgctatt tgcaacatta ttacgcagcg atgtttcatc attttcatca acccacagcg
    16321 tatccagggt gatttgccct tcgttatccg cggttgattt atcgatggct agcatcccta
    16381 cactgccacg gccatacata tcaataatac ccgtttcgcc gttcagaatg gatgacgagt
    16441 agctggcaag catacctgtc agccctaatg ccttgttatt gaaagaggta ccatcagaca
    16501 agttccactc tgccggataa ttcagcacac tgtgctgatt catcattttg gtggtcatgg
    16561 taccattgtt aacgccaatc ccaccgtatt caacatcaaa gacttttggt gccgggtcgg
    16621 taccaaaatc gtacatcgcc aggccaatgg atccgttatt gattaccgtt gaatctttat
    16681 tccaggcagc cgcaaaaccg atatttttca gttccacttc accgttgttt gtcgccttcg
    16741 caccagaatc aatactaatg gtggataacg ctacacgata ataataagtg aaagaatcca
    16801 catcactcat atcaaacgtt gcgtcagcgc caatctccag ctccgcgcca tggctcaact
    16861 gaatgagatt ttcgaacccc agcgggaact cgccgctgat ggttgactca ccattcaggt
    16921 agaccgggga atgcccatca acgttaataa gcggtgtgtc tccgatacca cgtcgagaag
    16981 ctgatggttt tgagccatca tccatgacat ttttttcacc gacaaaatgg acaccgccat
    17041 taagcgtaaa ggtattgtca tcgccagcaa taacaatacc tgcaatgtgt tcctgagcac
    17101 cgtcgtaact cgcataggaa cgcgacgtgg ttttactatc acccgaaaca ttaacacccg
    17161 tcgctttttg ccctgtcaca ttcagctcat aagtcgccag cgtcatgttg ttattattgc
    17221 cagagacatc aagaccggtg gagaaatccc caacgtctaa cccacccgcg agtgaaatat
    17281 tagctttatc cccagcaacg ctgacaccgg ttccggcatt ggtcgcggaa atatcaccaa
    17341 cgttaacaaa tgtcgcatta tcaccgtcga tctgaatgcc aatggcattt tcacccgctg
    17401 cggcctgacc aacattgagg tcgcccatgt tgatcacgct gacgccatca ccatcaagca
    17461 gcatgccaat agagttatct tcgatgaccg taatattacc tttgttgcta acgaacgtat
    17521 cattgccgtc aacgtcaata ccgtgcgcac caccgcttac gtataagtcg ccttcctgtt
    17581 tgatggtggc accgtcaccg acaattttcg agccagtgga attctctccg tcaacgctca
    17641 tggtaccttg attatcgacc cgcgcattat cgccattgat aaggctaccc gtaccaccat
    17701 tggtgatggt cgagtcgccc gcagtgttta gcttggtatt atcgccattg atgattaccg
    17761 cggtaccgcc atcaacttcc gtctcgccat tattgttaat ggtgatattg tcgccagtga
    17821 gtataccgac aaccgagcct tctccgatgg ccgtcgtttt gccgtcgttg ttgagggaga
    17881 tgttgtcacc atcaaccgtc atgacagtac cgccattcag cgccgtgttg ccgccctgat
    17941 cggtgatgat cgcgttacca aactcaatcg cattcccggt tgtgccgtca acaacgacgc
    18001 cggcctcttt agtgatgtgg atgagtccat catcgtcgta actccaggtg atgccacctg
    18061 aggtatttac gccatcaaag acaacggtgt tgctgtcgtc atggactgtc caggacttaa
    18121 ctatcgtctc cttgccctca ggcgccgtta aggtgtagct cccatcggca ttttggctgt
    18181 aggtaaaggt agtttcgcga attttgacag tttttgcatc ctgatcccaa gttacgccat
    18241 ttttatagac gacaggattg tgtttaaccg gggtatcgcc accatcgtct ggcggaactg
    18301 gcgtatcacc gccgtcatct ggcggaaccg gcgtatcacc gccgtcatca ggcggaactg
    18361 gcgtatcacc gccgtcgtct ggcggaacag gcgtatcacc gccatcatcc ggaactgggg
    18421 agttattatg gtggtgattg tcatcgtcac ttaaggtata tatagcaatt cccgtagcta
    18481 ctgcagcaag acctgcagct gcccatggta aaactgcact atctttattt tctctgcact
    18541 ccgcggataa attttctagc tgctctttgc tcagggaatg aatatttgcc ggacatgtta
    18601 tcttactgga cttacgtgtt ttttcggttg agtcgggtat tgaagtatcc gcagcccatg
    18661 tctggctact taacgccatg gcaacacaaa cagacaataa ttttttcctt tgcatataaa
    18721 attccttaga tctagaccgt gcgtaacaag ccatgcagaa tttgaagata aatcgtagta
    18781 aatattcata gtgaaataag cagtaaagaa atgcttatat aattcacaaa atcaatatta
    18841 aaatcaggca agtaaaag
//
LOCUS       DAZFTN010000087        18787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFTN010000087 DAZFTN010000000
VERSION     DAZFTN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(183..806)
                     /gene="rsmG"
                     /locus_tag="ACHKV2_003484"
     CDS             complement(183..806)
                     /gene="rsmG"
                     /locus_tag="ACHKV2_003484"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO6941308.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(870..2759)
                     /gene="mnmG"
                     /locus_tag="ACHKV2_003485"
     CDS             complement(870..2759)
                     /gene="mnmG"
                     /locus_tag="ACHKV2_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO6941309.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(3138..3581)
                     /gene="mioC"
                     /locus_tag="ACHKV2_003486"
     CDS             complement(3138..3581)
                     /gene="mioC"
                     /locus_tag="ACHKV2_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO6941310.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3671..4129)
                     /gene="asnC"
                     /locus_tag="ACHKV2_003487"
     CDS             complement(3671..4129)
                     /gene="asnC"
                     /locus_tag="ACHKV2_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO6941311.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4281..5273
                     /gene="asnA"
                     /locus_tag="ACHKV2_003488"
     CDS             4281..5273
                     /gene="asnA"
                     /locus_tag="ACHKV2_003488"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO6941312.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5278..6729)
                     /gene="viaA"
                     /locus_tag="ACHKV2_003489"
     CDS             complement(5278..6729)
                     /gene="viaA"
                     /locus_tag="ACHKV2_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO6941313.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6723..8219)
                     /gene="ravA"
                     /locus_tag="ACHKV2_003490"
     CDS             complement(6723..8219)
                     /gene="ravA"
                     /locus_tag="ACHKV2_003490"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO6941314.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8442..10310
                     /gene="kup"
                     /locus_tag="ACHKV2_003491"
     CDS             8442..10310
                     /gene="kup"
                     /locus_tag="ACHKV2_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO6941315.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10477..10896
                     /gene="rbsD"
                     /locus_tag="ACHKV2_003492"
     CDS             10477..10896
                     /gene="rbsD"
                     /locus_tag="ACHKV2_003492"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO6941316.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10904..12409
                     /gene="rbsA"
                     /locus_tag="ACHKV2_003493"
     CDS             10904..12409
                     /gene="rbsA"
                     /locus_tag="ACHKV2_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO6941317.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12415..13380
                     /gene="rbsC"
                     /locus_tag="ACHKV2_003494"
     CDS             12415..13380
                     /gene="rbsC"
                     /locus_tag="ACHKV2_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6941318.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13405..14295
                     /gene="rbsB"
                     /locus_tag="ACHKV2_003495"
     CDS             13405..14295
                     /gene="rbsB"
                     /locus_tag="ACHKV2_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO6941319.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14421..15350
                     /gene="rbsK"
                     /locus_tag="ACHKV2_003496"
     CDS             14421..15350
                     /gene="rbsK"
                     /locus_tag="ACHKV2_003496"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6941320.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15354..16346
                     /gene="rbsR"
                     /locus_tag="ACHKV2_003497"
     CDS             15354..16346
                     /gene="rbsR"
                     /locus_tag="ACHKV2_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO6941321.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16312..17739)
                     /gene="mdtD"
                     /locus_tag="ACHKV2_003498"
     CDS             complement(16312..17739)
                     /gene="mdtD"
                     /locus_tag="ACHKV2_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO6941322.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17762..18454)
                     /gene="yieP"
                     /locus_tag="ACHKV2_003499"
     CDS             complement(17762..18454)
                     /gene="yieP"
                     /locus_tag="ACHKV2_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO6941323.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4540 a         4641 c         4897 g         4709 t
ORIGIN      
        1 cgatttttag cagactgata ttttcactaa tgacttattt tctgcttacc aaaaaaagcc
       61 acgttatctt gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag ttatatcagt
      121 agaaaacctg gttgttgtta acagtctaac cggtcaattt tttatgattt ttttgataaa
      181 aattaaattt tatttgcttt aatcaccacc agatgacgtt cgccatccag ggctggaacc
      241 tgaagtttaa ccactgattc gacctgatat tcttcgggca acaaagcgat ttcatcttcc
      301 ggcatttgcc ctttcagcgc gtagaaacgg ccttgctcac caggaagatg gtggcaccag
      361 ctcaccatat cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct
      421 gaaggaaact cttctaccct gctctgtact ggctcaatat tctccagttt aagctcatgt
      481 tgcacctgac gaaggaaacg cacgcgttta ccaaggctat ccaacagagt gaaatgggct
      541 tcaggacgca cgatagagag tggaatgcca ggcagtccag gaccggtgcc gacatcgata
      601 aaccgttcac cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc
      661 atctcattag gatcgcggac cgaagtcagg ttgtacgctt tgttccattt atgcagcata
      721 ttcacgtagg caataagctg gtttttctgg tgatcggtaa gcgaaatacc tgcgtctttc
      781 agcagtaagg agagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcctggtaa
      841 gcgggggctt accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt
      901 ttcagccaca ccagcagaat ggagatggcc gcaggcgtga cgccagaaat acgtgaagcc
      961 tggccgatag aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg
     1021 gatacctggc ggtaatccag tgtcgcgggt agcaaggtgt tctcgttacg cagctgcttt
     1081 tcgatctcat cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt
     1141 tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca
     1201 taagtcattt ccggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgca
     1261 gtcaggtgag cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc
     1321 agacgctgac gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg
     1381 tcatccacca ggcccagttc acgaccgatt tcagtcaaac gcagatccgc attatcttcg
     1441 cgcagcatca gacgatattc tgcgcgcgag gtaaacatac gatacggttc tttggttcct
     1501 aaagtgcaca ggtcatcaac cagtacgccg agataggcct gagaacgcgc cggagcccaa
     1561 ccttctttgt catcagacag acgggcagcg ttaagaccgg ccagcaaacc ttgcgcagcg
     1621 gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac
     1681 ttgctctcca gcgtcggttt caggtcgcga ggatcgaaga agtcatactc aatggcataa
     1741 cccggacgca cgatcttcgc gttttccatc ccctgcatag agcggacgat ttgcatctgc
     1801 acatcgaacg gcaggctggt ggagataccg ttcggataaa tttcgttaga agtcagccct
     1861 tccggttcaa ggaagatctg atgctgattt ctgtcggcga agcgcatgac tttgtcttcg
     1921 atcgacgggc agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat tgggctacga
     1981 tcgaggttac tgcggatcac atcatgggtt ttctcgttgg tatgggtgat ataacacggc
     2041 acctgctgtg gatgctggga cgcattgccc ataaaagaga ataccggcat tgggttatcg
     2101 ccatgctgtt gcgcaagcac gctaaagtcg atggttcgag catcaatacg cggtggtgtc
     2161 ccggttttca gacgaccaac gcgcagcgga agttcacgca aacggcgaga aagcggaatg
     2221 gacggcggat caccagcacg gccaccgctg tagttatcca gaccgatatg aattttaccg
     2281 tcgaggaacg tcccaacggt gagcacgacg gctttggcac ggaacttcag tcccatttgg
     2341 gtaacagcac cgaccacacg atcgttttcg acaataagat cttcaaccgc ctgctggaag
     2401 atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg cctgacggta gagcacacga
     2461 tccgcctgag ctcgggtagc gcgaactgcc ggtcccttgc ttgcgtttag tatcctaaac
     2521 tggatacccg cctgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt
     2581 accagatgtc ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg
     2641 tcgatattgt gtgtcaaaag cagagtctgt tgacccatac gcgccgcagc catcgcggcc
     2701 tcggtgcctg catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg
     2761 gtgattgcct cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact
     2821 caactttgtc ggcttgagaa agacctggga tcctgggtat taaaaagaag atctatttat
     2881 ttagagatct gttctattgt tatctcttat taggatcgca ctgccctgtg gataacaagg
     2941 atccggcttt taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc
     3001 tggaccgtat aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacg
     3061 gttgttcttt ggataactac cggttgatcc aagcttcctg acagagttat ccacagtaga
     3121 tcgcacgatc tgtatactta tttgagtaaa ttaatccacg atcccagcca ttcttctgcc
     3181 ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc cgtctgtttt
     3241 gcaccggaat ttttgagttc agcctcgagt ttatcgatag ccccacaaaa tgtgtcatat
     3301 tcacgactac caataccgat tgcgccaaag cggaccgcag aaagatcggg cttctgttcc
     3361 tgcaatgctt catagaaagg agaaaggttg tccggaatat ctccggcacc gtgggtggag
     3421 ctgataacca gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt
     3481 tcggtggtaa aacccgcctc ttccagcttt tcagccaggt gttctgctac atattcggca
     3541 ccgccgaggg tgctgccgct gataagagtt atatctgcca taaaccgcca cctttattaa
     3601 gagtggcgta ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta
     3661 aaaaagccga tcagggcttg atggtacgca tgatcgggtt ctgcaggacg atcagtgtct
     3721 cggtggactg aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcat
     3781 cgatcgaacg gcacatcact tttataaaga tgctgtagtg gccggtggtg tagtacgcct
     3841 cggtcacttc atcgaggctt tccagctttg ccagcgcgga agggtagtct ttggcgctct
     3901 ttaatataat gccgataaag cagcctacgt cataaccgag ctgcttcggg ctgacatcaa
     3961 tacgcgcccc ggtaatgatc cccgcctgct tcattttctc tactcgaacg tgaatcgtcc
     4021 ccggactgac gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta
     4081 atgcttccag gatgccacgg tccagattgt cgatcagata attttccata ggattttctt
     4141 atgcggattg atgattcatt ctattttagc cttctttttt aatgaatcaa aagtgagtta
     4201 ggctttttat tgaatgatta ttgcatgtgt gtcggttttt gttgcttaat cataagcaac
     4261 aggacgcagg agtataaaaa atgaaaaccg cttacattgc caaacaacgc caaattagct
     4321 tcgtgaaatc tcacttttct cgtcaactgg aagaacgtct ggggctgatc gaagtccagg
     4381 cgccgattct tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag
     4441 cggtacaggt aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg
     4501 cgaagtggaa acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca
     4561 cgcacatgaa agcccttcgc cccgatgaag accgtctttc tccgttgcac tcggtctatg
     4621 ttgaccagtg ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc tcgactctga
     4681 aaagcacggt agaggcgatt tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag
     4741 agtttggcct ggcaccgttc ctgccggatc agatccactt cgtacacagc caggagttac
     4801 tgtctcgtta tccggatctt gatgccaaag ggcgtgagcg ggcgatagcg aaagatcttg
     4861 gtgcggtatt ccttgtcggg attggcggca agctgagcga tggtcatcgc cacgacgtgc
     4921 gcgcaccgga ttatgatgac tggagcaccc cgtcagagct gggccatgcg ggtctgaacg
     4981 gcgatattct ggtgtggaac cctgtactgg aagatgcgtt tgagctttct tccatgggga
     5041 tccgcgtgga tgccgacacg ttgaagcatc agttggcgct gaccggtgat gaagatcgtc
     5101 tgcagctgga atggcatcag gcgttgctgc gcggtgaaat gccgcagacc atcggcggcg
     5161 gtatcggcca gtctcgtttg accatgctgc tgctgcaact gccgcatatc ggccaggtcc
     5221 agtgtggagt atggccagct gctgttcgcg agagcgtccc ttctctgctg taataattta
     5281 tcgccgccag cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc
     5341 gaaaatgcgc atgatgccgg gtttgccgtg tgccgacatc gccacggcat gaaagcgatg
     5401 ctgatgtacc cgctgcagct ctttcacttt actcgtcacg tcgtcaggca accgctgggc
     5461 gataaaatca gaaatcacca ccgcatcggc atcaaaccat tccctgcttt gcaagcgttc
     5521 cataatggcg cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa
     5581 acggattgct tgttcgatgc cttgtgggcc tgaaagctca taacggacga tctcggtgga
     5641 aaatagcata atataacagc gccggttttc agcgagagca atgcgcatca aagccaggca
     5701 gaacgctttc gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacgcagac
     5761 aataaacggt ccgcgcggct gttcgtcgta atctttatgc accaccgggc gttcgatcac
     5821 tttttcacgc cacgactcac cgtgcaggcg ataggtgagc aactgttttt ccaccagccg
     5881 acggtaaaac tcatactcca gttccgttat ccctagtgtc gccagttctg gcggcagaag
     5941 acgtaaaata tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg tcgccggttc
     6001 gcgcaccatg gtgcggaagg tttccatctg cgcatcgttg cgcggtattg atttggcttc
     6061 ccgggaacgc cccagttgtt ctgccaggcg tttcagttcc ggctgttcgt taagaaattc
     6121 accgtatttc acaatcaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca
     6181 cagacgacca gctgcggtat tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt
     6241 catgcgttcc tgaacttcac tcaacagttg ttcgcgttct tcttctaata gctgttgatt
     6301 taacgttgtt gcttgcacga tcagacttaa acgccagcgc tggagaaaaa gcgtgtgtaa
     6361 cgctgaagtg atcgtgctgt tagcatcgac caactgtcgg gcttgttctg cccatgggga
     6421 attcagacga tgcagtaagt ccaggatctg tggtagctgc acaataaact gcggcgtgga
     6481 gaggagctgg ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc
     6541 atctttcagc cgactgcgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc
     6601 cttcagtcgt gggaattttt caaagaagac tgccagctgc ggtgaggcca gcagcgcgat
     6661 gatcatctct tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag
     6721 cgttagcatt gttgtgcctg gcgaatctgt gcgccgacat cctgtaggct ggcttcaatg
     6781 cgacctaacc agtcgccagg gataaacaga catctttgct gttcgctgaa taaagcgtgt
     6841 tgcttgcgcc agtcgctttc aagctcttcc aattgttgtt ttatttcacc tggcagacct
     6901 tcagccgatg aaccgggaag tgccagcgta ctgccttgta aacttacatc gcgtacaaca
     6961 agatgttggg cgctatcaac ttccagattc agtttctggg caaagccgat accgttcagt
     7021 ttgccgcgaa tttcaccacc tttgctcagc cactgttcca gcgcgctacg ctcaaaggag
     7081 atatgaacca cttccatatt atgcaatttt aacggttttt gcagcagcag agtcagagtg
     7141 gaagcagtaa cgttaacagg aagttgatac tgctgacgac ggctgaaaat gccgcccaga
     7201 cgaattaccg ttaaggctgt tttatcgctt tgttgctgct ggagttgcag gtgacgttgc
     7261 acaatcgcgc ccaggcgggt caacatccct tgctgttgcc aggcatgacc ggtcatcaat
     7321 acatcaattt gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc
     7381 agcaaaatga gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc gctggcctgc
     7441 aataatcgaa tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta
     7501 tccagttgct ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca
     7561 ccaatctctt tttgccagcg ttcatattct tcatctgtga cctgcaagga ggctggaacc
     7621 ggattgtcat tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcctgc
     7681 actttatcta accacagacg aatcagcatg cggtcatata acgcttccag actgctgtct
     7741 gcttccggca gctcgttgga ggccgccacc agcaggcgca tcgggatttt ttctacgtgt
     7801 gcaccgttgc ggaactggcg ctcgttaatg gcggtgagca gggtattaag aattgccgga
     7861 cccgctttcc agatctcatc cagaaagacg atttctgctt ccggcaggta accgctggtt
     7921 aaacgttcat agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacttct
     7981 tccggcgtgg agaagcgggt catcagatat tcaaacgcgc gggcattctg aaaggcgaat
     8041 tttaagcgcc gggcgatcaa acttttggca atacctggcg ggccaaggag gaacacactt
     8101 tcaccactta atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc
     8161 ttttccaacg aactgctcag gcgggaaatt ctttccgcta ataaatgagg gtgagccata
     8221 atgaagtggc gtcctttcgt caaaagttct gcgtaaattg cgagtataga cgtttcttgc
     8281 tggtggctaa aatagtctca aagggggggt atttttcttt gagccaggtt aatgtggccg
     8341 catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt gtgggccaag
     8401 ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact gataataagc
     8461 aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat attggtacca
     8521 gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc gttgaacgcg
     8581 atgccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg gtttccatta
     8641 aatatctcac cttcgtgatg cgggcagata acgccggtga aggggggatc ctgacgttga
     8701 tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg attatggggc
     8761 taatcggcgg cagctttttc tatggtgaag tcgtcataac acccgctatt tcggtgatgt
     8821 cagccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata gttcccctct
     8881 caattatcgt tctcacttta ttatttatga ttcaaaagca tggcacggct atggtcggta
     8941 agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcaggactg gggttacgta
     9001 gcattattgc taaccaggaa gtgctgtatg cgctgaatcc gatgtgggcg gtgcatttct
     9061 tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgctg tcgattacgg
     9121 gggtcgaggc gctgtatgct gatatggggc actttggtaa gttccctatt cgcctggcgt
     9181 ggttcaccgt cgtattacct tccttgaccc ttaattactt cggtcaggga gcgctgttgt
     9241 taaagaatcc ggaagcgatt aagaacccgt tcttcctttt ggcaccggac tgggcgctga
     9301 tcccgctgct gatcatcgcc gcactggcga cggtaatcgc ctcgcaggcg gttatctctg
     9361 gcgtcttttc attgacgcgt caggcggtac gtctgggata tttgtcgccg atgcgcatta
     9421 ttcacacctc cgaaatggag tcagggcaaa tctatattcc gtttgtgaac tggatgctct
     9481 atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg gcggcggcgt
     9541 acgggattgc ggtgaccgga accatggtgc tgacgtctat tctctcgact accgtggcac
     9601 gtcagaactg gcactggaat aagtattttg ttgcgctgat cttgattgct ttcctttgtg
     9661 tcgatattcc attgttcacc gctaacctcg ataaactgct ctccggcggc tggttgccat
     9721 tgagcctcgg tacggtgatg tttatcgtga tgaccacctg gaagagtgag cgtttccgct
     9781 tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct tcgctggaga
     9841 aatcaccgcc tgttcgcgtg cccgggaccg cggtatatat gtcgcgtgca atcaacgtca
     9901 ttccctttgc gctgatgcat aaccttaaac ataacaaggt attgcatgag cgggtgattc
     9961 tgttaactct gcgcaccgaa gacgctccat atgtccataa cgtccgtcgg gtacagattg
    10021 aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa
    10081 acgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg atgatggaaa
    10141 cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc
    10201 tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg
    10261 aaatcccgcc aaacagggtt atcgaactgg gtactcaggt cgaaatctaa cgccagacgc
    10321 ctcctttctt cataaggggg cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc
    10381 gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca ggattaaact
    10441 gcgggtcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac cgttcttaat
    10501 tctgatattt catcggtgat ctcccgtctg ggacataccg atacgctggt ggtgtgtgat
    10561 gctggtttac ccatccccaa aagtacaacg cgtatcgata tggcattaac ccagggtgta
    10621 ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt
    10681 atcgcggaag agatcaaaca ccataatccg caactccacg aaacgttgct cactcacctt
    10741 gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac gcatgaacaa
    10801 ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg ttctccgtat
    10861 gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag cattacttca
    10921 gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa
    10981 tgtctatccg ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta aatccaccat
    11041 gatgaaagtg cttactggca tctatactcg cgatgccggt acgcttttat ggctggggaa
    11101 agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga ttatccatca
    11161 ggaactgaac ctgatcccgc agttaaccat tgccgaaaac attttcctcg gtcgtgagtt
    11221 tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg ataaattgct
    11281 ggctaaactt aacctgcgct ttaaaagcga caagctggtg ggcgatcttt ccatcggtga
    11341 ccagcaaatg gttgaaatag ccaaagtgct gagctttgag tcgaaagtca tcattatgga
    11401 tgaaccgacc gatgcgctga ccgataccga aaccgaatcc ctgttccgcg tcatccgcga
    11461 gctgaaatcg caaggccgcg gtattgtcta tatctcccac cgcatgaaag aaatcttcga
    11521 gagttgcgat gacgttaccg tttttcgtga cggacaattt attgctgagc gcgaagtggc
    11581 atcactgacc gaagattcgc tgattgagat gatggtgggt cgcaagctgg aagatcaata
    11641 tccgcacctg gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc tctgcggacc
    11701 tggcgttaac gatgtctctt ttactttacg caaaggcgaa attcttggcg tctctggttt
    11761 gatgggcgca ggtcgtaccg aactgatgaa agtgctctac ggcgcactac cgcgcaccag
    11821 cggttacgtc accctggatg ggcatgaagt cgttacccgt tcaccgcagg atggcctggc
    11881 aaacggcatt gtgtatatct ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc
    11941 agtaaaagag aacatgtcgc tgacagcgct gcgctacttc agccgcgctg gcggcagttt
    12001 gaagcatgcc gatgaacagc aggctgtgag tgattttatt cgcctgttca atgtgaaaac
    12061 gccgtcgatg gagcaggcaa ttggtctgct ttccggtggc aatcagcaaa aagtggcgat
    12121 tgcccgcggt ctgatgacac gccccaaagt gttgatcctt gatgaaccta cccgtggcgt
    12181 agatgtcggc gcgaaaaaag agatctatca actgattaac cagttcaaag ccgatggctt
    12241 gagcatcatt ctggtgtcat cggagatgcc agaagtatta ggcatgagcg atcgcatcat
    12301 cgtcatgcat gaagggcatc tcagcgggga atttactcgt gagcaggcca cccaggaagt
    12361 gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa aaaaatgaca
    12421 acccagactg tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg
    12481 cttatcgctc tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc
    12541 atcaataact tattcaatat tctccagcaa acctcagtga acgccattat ggcggtcggg
    12601 atgacgctgg tgatcctgac gtcgggcatc gacttgtcgg taggttctct gttggcgcta
    12661 accggcgcag ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct
    12721 gccgctctcg cgttaggtgc cgcaattggt gcggtaaccg gagtgattgt agcgaaaggt
    12781 cgcgtccagg cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg
    12841 gtttatacca acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc
    12901 tggtttggta ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc
    12961 ttcctcgcgg cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg
    13021 ggcggcaacg aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc
    13081 gtctattctc tttgtggtct gctggcatca ctggccggga tcattgaagt ggcgcgtctc
    13141 tcctccgcac aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt
    13201 ctgggcggta cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca
    13261 ttaattcttg gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag
    13321 atgatcgtca aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa
    13381 taacgactac aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct
    13441 gttgcgctaa gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg
    13501 gtctccacgc ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg
    13561 gataaacttg gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg
    13621 gcgaacgtgc aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgaccgac
    13681 tccgacgcag taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact
    13741 cttgaccgcc aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg
    13801 ggtggcaaaa tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc
    13861 gagctgcaag gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag
    13921 gccgttgctg ctcacaagtt taatgttctt gccagccagc cagcagattt tgatcgcacc
    13981 aaaggtttga acgtaatgca gaacctgttg accgctcatc cggatgttca ggctgtattc
    14041 gcgcagaatg atgaaatggc gctgggcgcg ctgcgcgcac tgcaaactgc cggtaaatcg
    14101 gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt gaatgatggc
    14161 aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg cgttgaaacc
    14221 gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga tctgaaactg
    14281 gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa atacgtcatc
    14341 gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac acggtggcgc
    14401 attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt ggcagcatta
    14461 atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc gtaaccggta
    14521 accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta
    14581 gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc gagagcgttc
    14641 gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc aaaggcgaat
    14701 caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg
    14761 ccggcgctaa tgctgccctt tccccggcgc tggtggaagc gcaacgtgag cgtattgcca
    14821 acgcgtcagc attattaatg cagctggaat caccactcga aagtgtgatg gcagcggcga
    14881 aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct cgcgaacttc
    14941 ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc
    15001 tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg
    15061 aaaaaggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg gctagcgtga
    15121 atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc attgctgccg
    15181 gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca ttgccagagg
    15241 cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc gcacaacctt
    15301 ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga cgcttggcta
    15361 caatgaaaga tgttgcccgc ctggcgggcg tttctacctc aacagtttct cacgttatca
    15421 ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg attaaagaac
    15481 tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca cataccattg
    15541 gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt ggcgttgaac
    15601 gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc gatgaacagc
    15661 ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggcttg ctgttactgt
    15721 gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg
    15781 tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat aactcgttgc
    15841 tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt atcgcctgta
    15901 ttaccggccc gctggataaa actccggcgc gcctgcggtt ggaaggttat cgggcggcga
    15961 tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat tttgaattta
    16021 acggcgggtt tgacgctatg cgccaactgc tatcacatcc gctgcgtcct caggccgtct
    16081 ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag gcagagttac
    16141 aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca agctttatga
    16201 cgccaccatt aaccacgatc catcaaccaa aagatgaact gggggagctg gcgattgatg
    16261 tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa cttactccga
    16321 ttctgatgga acgtggttcg gcttagattt acgctgtctt ttgatcaaat tattaccatc
    16381 ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc ccattgtgat
    16441 aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc attccttcat aaacgcgaag
    16501 gacggccgca cttacagcta cgcctaaact gatagacagt tgctgcgtga ccgccagaac
    16561 actgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg tattcatcgc
    16621 ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca gcatccatat
    16681 tgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa tcaccgtgat
    16741 ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca tcgattttgc
    16801 aataatggaa cctaacgctg tcggtgccat catacagcca gcaataaacg cctgataacc
    16861 aaatcctacc tgtaacatta atggcataag gaacggtaca cagccggtcc ccagacgggt
    16921 tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat ctaatgaaat
    16981 taatgggttt ggcgtgcgtc gtgcatggag aatatagaga agcagtaacc cgatgctggt
    17041 gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata gctctattcc
    17101 gcttgagaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat cgaatctgcg
    17161 tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg caatacctat
    17221 ggggatattg attaaaaata tccagtgcca ggttgcccag gtgaccagca cgccgccaag
    17281 aatggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca atactggaag
    17341 aagttcatta cgaggataag cgcgcagtaa ggccagccga gcaacaggca tcatcattgc
    17401 gccgcctatc ccctgaataa cccggaagac aaccagctgt ggtagcgaat tagaaagtgc
    17461 gcaggccaga gaacccaatg tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc
    17521 gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca gcgtataact
    17581 gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga ttgcgggtaa
    17641 ggcggtattc agaatagtgg catcaagtgc ctgcatgaag aaggccatcg ccgcgatcca
    17701 cggcaaaccc gccatactgc gcttcttttt atcgctcatt caatgtcctg ttatcgggtt
    17761 atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc
    17821 tttggataat cgcatcgact atcgcctgat gcagatccag ctttatcact gtgtcgctgg
    17881 taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa
    17941 acggattggc gctcatttca tagatatgct catgccaggc catatcgact tcgatccagc
    18001 gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta ttgagatgcg
    18061 ctttctgttc cgcggtgcca accgttgctg ccagtaggca agcttgtggc tccagacaga
    18121 tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc atccaccagg
    18181 taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccgg gtaccaattc
    18241 gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg
    18301 tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg gtgccgggtt
    18361 catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac gaaaggtttt
    18421 tctgtgcagc taactgttgt gcgcttaaag gcattactaa tcttcctttt tctttttatt
    18481 cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa attgcttgtt
    18541 ttatggcaca ttaacggggc ttttgctgaa aaaatgcgcg gtcagaaaat tattttaaat
    18601 ttcctcttgt cagaccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
    18661 caagcacgcc gccgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
    18721 aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc ccgcgccgct gagaaaaagc
    18781 gaagcgg
//
LOCUS       DAZFTN010000088        18437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFTN010000088 DAZFTN010000000
VERSION     DAZFTN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..683
                     /locus_tag="ACHKV2_003500"
                     /pseudo
     CDS             66..683
                     /locus_tag="ACHKV2_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            786..1289
                     /gene="yfbM"
                     /locus_tag="ACHKV2_003501"
     CDS             786..1289
                     /gene="yfbM"
                     /locus_tag="ACHKV2_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO6941324.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1356..2813)
                     /gene="nuoN"
                     /locus_tag="ACHKV2_003502"
     CDS             complement(1356..2813)
                     /gene="nuoN"
                     /locus_tag="ACHKV2_003502"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO6941325.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2820..4349)
                     /gene="nuoM"
                     /locus_tag="ACHKV2_003503"
     CDS             complement(2820..4349)
                     /gene="nuoM"
                     /locus_tag="ACHKV2_003503"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO6941326.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4580..6421)
                     /gene="nuoL"
                     /locus_tag="ACHKV2_003504"
     CDS             complement(4580..6421)
                     /gene="nuoL"
                     /locus_tag="ACHKV2_003504"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO6941327.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6418..6720)
                     /gene="nuoK"
                     /locus_tag="ACHKV2_003505"
     CDS             complement(6418..6720)
                     /gene="nuoK"
                     /locus_tag="ACHKV2_003505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO6941328.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6717..7271)
                     /gene="nuoJ"
                     /locus_tag="ACHKV2_003506"
     CDS             complement(6717..7271)
                     /gene="nuoJ"
                     /locus_tag="ACHKV2_003506"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO6941329.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7283..7825)
                     /gene="nuoI"
                     /locus_tag="ACHKV2_003507"
     CDS             complement(7283..7825)
                     /gene="nuoI"
                     /locus_tag="ACHKV2_003507"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO6941330.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7840..8817)
                     /gene="nuoH"
                     /locus_tag="ACHKV2_003508"
     CDS             complement(7840..8817)
                     /gene="nuoH"
                     /locus_tag="ACHKV2_003508"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO6941331.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8814..11540)
                     /gene="nuoG"
                     /locus_tag="ACHKV2_003509"
     CDS             complement(8814..11540)
                     /gene="nuoG"
                     /locus_tag="ACHKV2_003509"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO6941332.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11593..12930)
                     /gene="nuoF"
                     /locus_tag="ACHKV2_003510"
     CDS             complement(11593..12930)
                     /gene="nuoF"
                     /locus_tag="ACHKV2_003510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO6941333.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12927..13427)
                     /gene="nuoE"
                     /locus_tag="ACHKV2_003511"
     CDS             complement(12927..13427)
                     /gene="nuoE"
                     /locus_tag="ACHKV2_003511"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO6941334.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13430..15232)
                     /gene="nuoC"
                     /locus_tag="ACHKV2_003512"
     CDS             complement(13430..15232)
                     /gene="nuoC"
                     /locus_tag="ACHKV2_003512"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO6941335.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15326..15988)
                     /gene="nuoB"
                     /locus_tag="ACHKV2_003513"
     CDS             complement(15326..15988)
                     /gene="nuoB"
                     /locus_tag="ACHKV2_003513"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO6941336.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16004..16447)
                     /gene="nuoA"
                     /locus_tag="ACHKV2_003514"
     CDS             complement(16004..16447)
                     /gene="nuoA"
                     /locus_tag="ACHKV2_003514"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO6941337.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17078..18016)
                     /gene="lrhA"
                     /locus_tag="ACHKV2_003515"
     CDS             complement(17078..18016)
                     /gene="lrhA"
                     /locus_tag="ACHKV2_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO6941338.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4602 a         5125 c         4734 g         3976 t
ORIGIN      
        1 ataaataatg ataaaataac gtcttttatt gataatttac atatgaaaaa aattttcctt
       61 ttgttatatt atttctgttt tcactccctc tgattatttt ttaccaaccc tgggtcactg
      121 cattatcgcc aacgccccga cacgtaattc ctgagcaatt agaaaaaaca gttcgttatc
      181 ttacacaaac tgtgcatcca cgtaatgccg acaatattga taatctgaat aggtccgtag
      241 agtatataaa aaaaggtctt tgtcagtagc ggtgccagag ttaccgcgca gggcgtcccc
      301 aattggctat tacgactctg cgcctggtag ccaggattac ccttacccgc aatgtcctgg
      361 ctttatcccg atcgggggga ctttattgcc tggtcggcag aatacaggat atcaacgccg
      421 ttcgtcaggt aaaaacggca ttgttatcat cccgggattt atctgtttat tctatgaatg
      481 ccccatggtt tattcacggt attgatttct ctgaccacct taattattgg caacacgata
      541 ttctcgccgt aatgattact gacaccgctt tttatcgtaa taaacaatac cacttgcccg
      601 gtgatacagc agacagattg aattatcaga aaatggctca gatggtggat ggtgttataa
      661 ctttgttaca caacagtaaa taataaaaat ttctcgaggc gggtccagaa acagcgacat
      721 gtacatttta ttttccctcg tctgttttaa gatgcgaaac ataaaattca atggaggaaa
      781 aataaatggg aatgattggt tattttgcag aaattgactc agaaaagatt aaccaactgc
      841 ttgagtctac tgaaaaacct ttgatggata atatacatga cacgctttca ggattacgcc
      901 gactggatat tgataaacgc tgggattttt tacactttgg gctgacaggc acctctgcgt
      961 tcgatccagc gaagaatgat ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag
     1021 atggtataga tggtttcctg ggattgacat ggaatcagga gttagccgcg acaatcgacc
     1081 gactggaatc actggatcga agtgagctgc gtaaaaagtt ttccattaag cgactcaatg
     1141 aaatggagat ttatccggga gtgacctttt cagaagagtt agaggggcaa ctctttgctt
     1201 cgatcatgct tgatatggaa aagctgatta gcgcgtatcg acgtatgctg cgacaaggga
     1261 atcatgcgct gacggttatt gttgggtgat ggtgtcaggg caccaaaaaa aaccgccgaa
     1321 catgtcggcg gctttctgac ttacaaactg atagcttaca tcagcggcat tgctaaccgc
     1381 acaatactaa tcagcggttg tggccataca cccagcacca gtaccaacag tgcagaaatc
     1441 agcaccacga taccgcccgc gctgtactgc cagtttgacg gtgcatcgcg acccggttgt
     1501 tccggtgcgt gcaggtacag gctcaccgcc acgcgcaggt agtagtagag gccgattgcc
     1561 gaaccgacaa ccacggcacc caccagccac cacaggtgtg cctggacacc aaccgccagc
     1621 acgtagaact taccgataaa gcccagcgtc atcgggatac cggccagaga cagcatcatc
     1681 accgtcatca ccgccgcgag gatcggacga tgccagaaca gaccgcggta agagaacagg
     1741 gaatcagcat ccgggccacg atacgggctg gacatcaggc tgaccacgcc gaacgcgccg
     1801 aggctgctga acagataacc ggccaggtaa acccctaccg cttccatcga catctcgcca
     1861 gtttgcagcg caatcagcgc caccagcaga tagccgaggt gagagataga tgagtaaccg
     1921 agcagacgtt tgatattggt ctggctcagc gccatcaggt taccgaagat gatggaggca
     1981 aaggcgataa tcgccagcac cacccgaatc gcttcgctgt cacccaccgg tgcgtagagg
     2041 aacagacgca tcaccacacc gaagatagcg attttgctcg ccgtcgccag gaaagtggaa
     2101 accggcgcag gcgcgccctg gtatacgtct ggcgtccaca ggtggaacgg caccagagag
     2161 agtttgaagc cgaggccaac aatcatcagg ccgaaacctg ccagcaacag cggctcgttt
     2221 agcataccgt cgccgaggtt tttacccagc gcgacaaacg acaggtcgcc agactgcgca
     2281 tacaccagcg ccataccgaa cagcaggaaa gaagacgctg cggcagaaag gatggtgtat
     2341 ttgatactgg cttccagcga acgtttctgg cggaaagcgt aaccgaccag gccaaacagc
     2401 ggcaaagaga tcagttcaat accgaggaac agagacgcca gatggtttgc attcgccagc
     2461 aggatcccgc ccagcgcggc aattaacacc aacaggtaga actcatcctt gttgtcgtta
     2521 tagccttcaa gccacgggta ggcgaaagta caggtggcga ggctcgccaa caataccagc
     2581 ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg gcgtaacgtc catagcgccc
     2641 gcctggccaa caaaccagag cgaaaccagc gccgcgttaa gcccaataac cgagagcgta
     2701 gcgttgagga aatgattgcg tcgccacgca atggagagca tcacaaccac caccgtcaag
     2761 ccgacgatca gcaacggtag cagtgcgatc aggttttgtg gagttattgt catggcgatt
     2821 tacggccttg tagtagtaac ggaattaaca aaccactgct ggatattgcc aatcgcggag
     2881 tgcgaggtat ccagaatcgg ctgcggatag aagcccagca gtaccagcag caccaccagc
     2941 aacaggatca taaacagctc acgcagcgac atccctggca gttcctggct ggcaatctgg
     3001 cttttcgctt taccgaagta agcgcgatgt aacatcgcca gcgaataaac agatgcaaag
     3061 accagcccaa aggtagagat aacggtaatc accgggacaa cctggaagct gccgaacaga
     3121 atcataaatt cgccgacgaa gttaccggtg cctggcatcc cgagcgttgc caccgcaaag
     3181 aacagtgaca aggctggcag ccatttcatc ttgctccaca gaccgcccat catgcgcatg
     3241 tcgcgggtat ggatacgttc ataaagctga ccacaaagaa taaacagacc cgccgctgac
     3301 aagccgtgcg caatcatctg gattaccgcg ccctggtagg ccaactggct gccggtgtag
     3361 atagcaatca gtacgaagcc catgtgggaa accgaggtgt aggcgatcag acgtttgata
     3421 tcggtctggg cgaaggccat ccacgcaccg tagaagatgc cgataacacc cagccacatg
     3481 gcgattggcg cgaactctgc cgacgcgttt gggaacagcg gtagggagaa acgcagcaga
     3541 ccgtaagcgg cggttttcag caagatcccc gcgaggtcca cggaaccggc ggtcggtgcc
     3601 tgtgagtgcg catccggcag ccagccatgc agcggaacca ccggcatttt caccgcaaac
     3661 gcgatgaaga agcccagcat caacagatat tccacaccac tggacattgg cgtattcagc
     3721 agctcttcat agttgaaggt ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc
     3781 aggatggcga tcaacatcac cagaccactc gcctgggtgt aaatgaagaa cttggttgcc
     3841 gccgtgatac gcgttttacc atcagaggct ttatgccccc acagtgcgat caggaagtac
     3901 atcggcacca gcatcatttc ccagaagaag aagaacagga acatgtcgat ggcaaggaac
     3961 acgccgataa cgccgcccag gatccacatc aggttgaggt ggaagaagcc ctgatatttt
     4021 tcgatctctt tccacgaaca gagtaccgcc agcacaccga gcagaccggt cagcacgacc
     4081 atcagcagcg acagcccgtc aatggcgaga tggatagaga taccaaaacg cgggatccac
     4141 ggcatgtcga attcagactg ccactgcgga attccggcgg attgcgtcag tgaataaccg
     4201 ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc
     4261 cagcgcggca ccttgacgcc aaagcgttcg gtctgccagc acaggaagcc gccgataaag
     4321 gggattagta ttaaccaggg taataacata gctaagtatg tcccttatat ttactcctgc
     4381 tcaggctatc tcactcgcca gcccgaatcc cggcagtgct cgcaatcctc acgtacttaa
     4441 gtacgctccg gttgctgtgc gctggcggta tccggtctga cttcgccgat gacgcctgta
     4501 cttcaggaac agttacctaa aatttattct tagttcacag acccggcgca acgccgggta
     4561 tattcactca gccaacaact caacgcagta ccatcaacag tgccagcacc acgaccgcac
     4621 cgatgctcat ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcaggcctt
     4681 tacctgcaaa gcgggaaagg acagccggga tgttcatcat tgagttcagc ggatcgcgtt
     4741 tcagcaacca ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa
     4801 agccccaggc gttgtaccac caggtgccca gcagacggcc cggcgcactg ttggcgatgg
     4861 aggtcaccag agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg
     4921 cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt
     4981 gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca
     5041 gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt
     5101 cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac
     5161 cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct
     5221 catccttact gaagaagccc gcagtgacta gcggcagtgc cgacagtgct gcgccgccca
     5281 ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt
     5341 tctgttcgtg atggcaggcg agaatgacgg aaccggatgc caggaacagc agcgctttaa
     5401 agaacgcgtg ggtcatcaag tgaaaaatcg ccgcatccca tgcctgcacg ccaagcgcga
     5461 ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct
     5521 gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccaacaatg cccaccagat
     5581 gcagaacttc cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg
     5641 cggttaccat cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt
     5701 cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca
     5761 tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca
     5821 ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagc gcgaaagcga
     5881 ggaacacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg
     5941 gatcggtgta atagaacccg atcagcagat aggagcacag gcccacgcct tcccagccga
     6001 ggtacatcag cagcaggttg tcggcaagca ccagaaccac catgctggcg ataaacaggt
     6061 tggtgtaagc gaagaagcga gagtagccct cttcaccgcg catataccag gaggcgtaca
     6121 tgtgaataag gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca
     6181 gcaccaggtt aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc
     6241 tgtatgcctg ctcgccgtta gcgaagaagt caacgccaat aaaggcagtt accagcgccg
     6301 ccaggcccac agagccaacg ccgacgatcg ccgagacgtt ttcagaccag cgcccacggg
     6361 agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca
     6421 tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag
     6481 cagcagcgca aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat
     6541 cacctgaccg tcggtctggc cccagtaact tccggcgacc acaaaggcca gcgcggaagc
     6601 gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc
     6661 ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat
     6721 gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc
     6781 gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa
     6841 gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg
     6901 attggcgtac cgtcgatacc ttgatcgtta acaccgagaa tggcgtaaac aatcaccacc
     6961 aacatgatgg ctgacaaaat tgccggacca atccacacct gcggtttcag ccactggcgt
     7021 tcttgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc
     7081 ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag
     7141 aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga
     7201 ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa
     7261 gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt
     7321 cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt
     7381 tatactccgg gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat
     7441 cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac
     7501 aggcttcttc gcacaaacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg
     7561 gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg
     7621 ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa
     7681 cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt
     7741 cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac
     7801 ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat
     7861 gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa
     7921 ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat
     7981 cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca
     8041 gccaccgaag aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc
     8101 cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc
     8161 ctgctcggct tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc
     8221 aaaggtaata aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac
     8281 gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag
     8341 gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcaccgagca gcgagtattt
     8401 gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagaccgg ccatcatcag
     8461 gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat
     8521 cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg
     8581 atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag
     8641 ctggagcgaa ccaccccagc caacacggtt aggtccgtaa cggttctgga acagacccag
     8701 caggcgacgt tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca gcaggatcac
     8761 caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt
     8821 tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata
     8881 cccatcggca agcccacctg ccctgccgtc agcccttcag cgatttcaac cggcagagtg
     8941 accgtgttgc catcgtaact aaaggagacg cgtgtacctg cgtttacacc caacttcgcg
     9001 gcatccgctg ggttgagttt gatgtacggc tgcggcatac gacactggaa gaccggagca
     9061 cgctgtgaca attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg
     9121 tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt ttcgctggtt
     9181 tcaaacagac gcacacccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat
     9241 ttgttccacg cctgcgggga gttccagccc ggagcccagg caaacggcac ttgcgaacgg
     9301 tgcgcggtcg gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc
     9361 ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg
     9421 tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatctgg cgcagcatct
     9481 ttgatacccg ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc
     9541 gtccagtcca cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccaactt
     9601 tccagcatga cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg
     9661 gcgcgacctt cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc
     9721 accagatggg cgttttccat gatcgctgtg cgttgatggt caaccaccat caccagcggc
     9781 gctttagcca gcgcagcatt cacgcgggta gcagaagcgt gacgatgcag atcgttttcc
     9841 agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgat
     9901 ccaccgccca taatgcccaa ccccatgctg ttgacggaac gggcaatcat ggtgataccg
     9961 acgtcagcgc cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct
    10021 aagctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacccgc cagtgcctgc
    10081 acgatgacgt cgattttgct ttgcagctca ggttcaatac cgtcaaccgc tggcgcagag
    10141 ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg
    10201 taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga
    10261 tgcttagcac gttgaccgat attgaggatt gccgcaatct gccagtcagc cactttctgt
    10321 gctgccgcca tttcgcgcgc tttacctttc accgcctggc gcactgccag cgcgacgcgc
    10381 gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg
    10441 cgcagagccg gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt
    10501 tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc
    10561 gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga
    10621 cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg
    10681 ccacgacgct gtactggctg acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa
    10741 cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga
    10801 cgcagttcgc cataacgctc gccggggctg atgttacagc cgatggaaca ttgctggcag
    10861 atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg
    10921 gtaaatacgc cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg
    10981 ccgtcttccg ggcgaccgaa gtagacgttg tcatgcgcgc cataaacgcc cagatctgta
    11041 ccgtcagcgt aatctttgta gtaacgcacg cagcggtagc aggcgatgca gcggttcatt
    11101 tcgtgagaga tgaacggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag
    11161 cgacggaagc tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct
    11221 tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg
    11281 aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata
    11341 caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac
    11401 tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg
    11461 cccagagaca gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg
    11521 tctacatgaa ttgtagccat tagcatgctt ccagttttct cagtcagaga ctgagcgtta
    11581 atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg
    11641 ggtattgctg aacggctgtt tgatccccgc ctcaaattct tcgcggaaat atttgatggc
    11701 gctctgtaac ggctccacag caccaggtgc gtgggcacag aaagttttac ccgggcctaa
    11761 gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tgaccttcgc cacgctccag
    11821 cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca
    11881 ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatttcatg
    11941 gtcaaccgcc atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa
    12001 ttccatcggc aggtcaaggt gcgcttcggt caggaagtcg gtgcccgcgc cgcctggctg
    12061 ccaggctttg aatttcagac cgtcacgcat accaccggcg taatcttcga ggatctcgcg
    12121 tgcggtggtg ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc
    12181 catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt
    12241 agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc
    12301 ccatacgccg gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc
    12361 cagggagttg attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac
    12421 gaacagttcg aagtcgaaac ctgttcccat aatgtttttg ccaagcaatc ccgcttcagt
    12481 cgcttcggca atggcgcggc gcagatttac tgccgcttca atatattcgc cacgcaggaa
    12541 gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag
    12601 gtgcggcagt tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc
    12661 gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcaa
    12721 gccagtcgaa aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac
    12781 gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct
    12841 gcggtattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg
    12901 cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct
    12961 tccggggtca gattcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag
    13021 ttccccaggc agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt
    13081 ttgatgttca gctttttctc gagcgccgcc tgaataccct gatacccatt gatatgacag
    13141 accacgctgt cgcaataacg gatcacatgg cggccaaccg gctggcggaa gatctggctg
    13201 tagaacgttg ccacgccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg
    13261 tggatcgcac catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg
    13321 gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct cgatcgcttc acgctctgcc
    13381 gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca
    13441 catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc
    13501 cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc
    13561 gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatccct ttggtcgcct
    13621 caatcatctg gaaggattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt
    13681 gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg
    13741 ggtgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca
    13801 gactctggcg cagctcttcc actttaagca ttacgcgggt gtagcagtca gaaacaccac
    13861 caccaaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca
    13921 cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct
    13981 ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggta ttttgcagcg
    14041 ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcaggc
    14101 gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca
    14161 tacggaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga
    14221 agaacacggg cgtcattgcg ccgacgtcct gaataaaggt cgagatatac agcaggtggc
    14281 tgttgatgcg gaacagttcg gagagcatta cgcgaatgac gttaacgcga gccggcacgg
    14341 tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga
    14401 ggtattcgat acggtcggta tacggaatgt agctgtgcca ggactggcgt tcgcccattt
    14461 tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt
    14521 cgagctgcaa aacgatacgg aaagcaccgt gcgccgacgg gtggttcgga ccgaggttga
    14581 ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg
    14641 tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg
    14701 tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga
    14761 tgcggcgcag gttcgggtga ccgtcgaatg taatgccaaa cagatcccag gtttcacgct
    14821 cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg
    14881 ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa
    14941 cggaaaaatc cgcggcaggt aacccttcgc ggtgcgtgcg cagacgttcg tccatgccgt
    15001 gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca
    15061 gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa
    15121 aggcatccgg cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc
    15181 gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg
    15241 gtccgtggtg actgtcggcg cagcaagctt cgcgaaatag atttaggaat aagcgaagaa
    15301 aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg
    15361 caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct
    15421 gatcgccaac cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca
    15481 tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg
    15541 ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg
    15601 cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa
    15661 taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg
    15721 gcgaagcacg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag
    15781 tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac
    15841 gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct
    15901 ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg
    15961 gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag
    16021 cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca
    16081 gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag
    16141 cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca
    16201 gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc
    16261 gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggcacgttt ttcgacctcg
    16321 cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacat aggccaatgg
    16381 caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca
    16441 tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac
    16501 gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt
    16561 atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa
    16621 agtttcttca cattagttta cataatatca acaccattag catttaatgc cctttcaccc
    16681 cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa
    16741 tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga
    16801 gtattttctt gatctgtgac acgcttttgt cattccataa caaaaacgca gcaacaaatt
    16861 tacgtatttt ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgtac
    16921 ggggcgtgaa tctctggtga ttgaaaaatg aacagttttg tacgttctgc acgatgtgaa
    16981 gaaggttctt tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt
    17041 tacactattg tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca
    17101 acaatgaatc atccccttcc ggagcagata ttgggctgta ttgccacgga ttatggtagc
    17161 tctccatcgc ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca
    17221 gcaaatattc ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaagt
    17281 ccggactcat catctcgacc ggccttgccg tcacgccaag accggctttc actgccgcac
    17341 gaacggccgg aagcgtcgag gcgacataag ccagtcgcca gggaatatct gctttattaa
    17401 gcgtcgccag caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg
    17461 gctcaccttt ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca
    17521 ggttcaatgc cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg
    17581 attccagcat ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg
    17641 gataaaccga actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg
    17701 aagcaccgat agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat
    17761 cattaaaacg caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca
    17821 gtttgttgcg accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca
    17881 tttgctgact tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt
    17941 tcagatcggc aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac
    18001 gatttgcact tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg
    18061 tgcacacaca aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac
    18121 ccggacgaaa gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt
    18181 ataatgcctg aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact
    18241 cactcttttc tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc
    18301 cactctcgtg cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaggcgg
    18361 catcaagagc agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat
    18421 aagggtcatt gcgaatt
//
LOCUS       DAZFTN010000089        18346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFTN010000089 DAZFTN010000000
VERSION     DAZFTN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..669
                     /gene="ecpR"
                     /locus_tag="ACHKV2_003516"
                     /gene_synonym="matA"
     CDS             127..669
                     /gene="ecpR"
                     /locus_tag="ACHKV2_003516"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO6941339.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            744..1331
                     /gene="ecpA"
                     /locus_tag="ACHKV2_003517"
                     /gene_synonym="matB"
     CDS             744..1331
                     /gene="ecpA"
                     /locus_tag="ACHKV2_003517"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO6941340.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1389..2057
                     /gene="ecpB"
                     /locus_tag="ACHKV2_003518"
                     /gene_synonym="yagY"
     CDS             1389..2057
                     /gene="ecpB"
                     /locus_tag="ACHKV2_003518"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO6941341.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2083..4608
                     /gene="ecpC"
                     /locus_tag="ACHKV2_003519"
                     /gene_synonym="yagX"
     CDS             2083..4608
                     /gene="ecpC"
                     /locus_tag="ACHKV2_003519"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO6941342.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKNRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4598..6241
                     /gene="ecpD"
                     /locus_tag="ACHKV2_003520"
     CDS             4598..6241
                     /gene="ecpD"
                     /locus_tag="ACHKV2_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO6941343.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6210..6920
                     /gene="ecpE"
                     /locus_tag="ACHKV2_003521"
     CDS             6210..6920
                     /gene="ecpE"
                     /locus_tag="ACHKV2_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO6941344.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7233..7562
                     /gene="ykgJ"
                     /locus_tag="ACHKV2_003522"
     CDS             7233..7562
                     /gene="ykgJ"
                     /locus_tag="ACHKV2_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6941345.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7810..8424)
                     /gene="yagU"
                     /locus_tag="ACHKV2_003523"
     CDS             complement(7810..8424)
                     /gene="yagU"
                     /locus_tag="ACHKV2_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO6941346.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8842..9531
                     /gene="paoA"
                     /locus_tag="ACHKV2_003524"
     CDS             8842..9531
                     /gene="paoA"
                     /locus_tag="ACHKV2_003524"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO6941347.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9528..10484
                     /gene="paoB"
                     /locus_tag="ACHKV2_003525"
     CDS             9528..10484
                     /gene="paoB"
                     /locus_tag="ACHKV2_003525"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO6941348.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAIRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYTFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10481..12679
                     /gene="paoC"
                     /locus_tag="ACHKV2_003526"
     CDS             10481..12679
                     /gene="paoC"
                     /locus_tag="ACHKV2_003526"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO6941349.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12689..13645
                     /gene="paoD"
                     /locus_tag="ACHKV2_003527"
     CDS             12689..13645
                     /gene="paoD"
                     /locus_tag="ACHKV2_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO6941350.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(13824..14951)
                     /locus_tag="ACHKV2_003528"
     CDS             complement(13824..14951)
                     /locus_tag="ACHKV2_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6941351.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(15093..16151)
                     /locus_tag="ACHKV2_003529"
     CDS             complement(15093..16151)
                     /locus_tag="ACHKV2_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6941352.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16397..17299
                     /locus_tag="ACHKV2_003530"
     CDS             16397..17299
                     /locus_tag="ACHKV2_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6941353.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            17536..17598
                     /locus_tag="ACHKV2_003531"
     CDS             17536..17598
                     /locus_tag="ACHKV2_003531"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941354.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(18002..18280)
                     /locus_tag="ACHKV2_003532"
     CDS             complement(18002..18280)
                     /locus_tag="ACHKV2_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941355.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
BASE COUNT         4622 a         4884 c         4772 g         4068 t
ORIGIN      
        1 atgtgctacg tatcttattt acttccgatt tactaaagaa actgaatgta cctgtaaaaa
       61 ttacaggttt ggaaagtagt gacatggcaa aatgattaca gcagggacta tgaggttgaa
      121 aaccatatgg aatgtcaaaa ccgttctgat aaatacatct ggtctcccca tgacgcctac
      181 ttctataaag gactatctga actgattgtg gatatcgaca gattaattta tctatcgctg
      241 gagaaaatca gaaaagattt cgtgtttatc aatctcaata cggattcttt aactgaattt
      301 ataaaccgtg ataatgagtg gttatccgcg gtaaagggga aacaggtcgt attgattgcg
      361 gccagaaagt cagaagcctt agcaaattat tggtattaca acagcaatat taggggcgtg
      421 gtatacgctg gactgagtcg tgatattaga aaagaactgg cctatgtgat taatggcagg
      481 ttcctgagaa aagatattaa gaaagataaa atcactgacc gggaaatgga aattatccgc
      541 atgacggctc agggaatgct gcctaaatcg attgccagaa ttgaaaattg tagtgtaaag
      601 acagtgtata cccatcggcg taatgcagag gccaagctgt actcaaaatt atataagttg
      661 gttcagtaaa ctccaggcaa gttagtttta aaaaatgact cactgggaca tcacgtcctc
      721 aattcaactc gggaagaaat acaatgaaaa aaaaggttct ggcaatagct ctggtaacgg
      781 tgtttaccgg catgggtgtg gcgcaggctg ctgacgtaac agctcaggct gtagcgacct
      841 ggtcagcaac agccaaaaaa gacaccacca gtaagctggt tgtgacgcca ctcggtagcc
      901 tggcgttcca gtatgccgaa ggcattaaag gttttaactc acagaaaggt ctatttgacg
      961 tggctatcga gggtgactca acggctaccg cctttaaact gacctcacgt cttatcacca
     1021 acacattaac ccagttggat acctcaggtt ccacactgaa tgtgggcgtg gattataacg
     1081 gcgcggcagt cgaaaaaact ggcgataccg tgatgatcga taccgccaac ggcgtactgg
     1141 gcggcaacct tagcccgctg gcaaacggtt acaatgccag caatcgtacc accgcacagg
     1201 atggtttcac tttctccatc atcagcggca ccaccaatgg taccaccgca gtaaccgatt
     1261 acagcactct accggaaggc atctggagcg gcgacgttag cgtacagttc gacgcgacct
     1321 ggaccagtta atctctctga tgtaccagca ggggtagccc cccagcttta ttcctggacg
     1381 gaattgttat gaaaaagcac cttctgcttc tcgctctgct gttatccgga atatctccgg
     1441 cccaggcgct ggatgtcggc gatatatcat cgtttatgaa cagtgacagc agcacgctga
     1501 gcaaaacgat caaaaacagt accgacagtg gtcgccttat caatatccgt ctcgaacggc
     1561 tctcttcacc gcttgacgac gggcaggtta tctcaatgga caagccggac gagttgctac
     1621 tcactcccgc cagcttgctg ctacccgccc aagccagcga agtgatccgc ttcttctata
     1681 agggaccagc agatgaaaaa gagcgctact accgcattgt ctggtttgat caggccctca
     1741 gtgatgcgca gcgcgataat gccaaccgca gcgctgtggc cactgcttcc gcccgcatcg
     1801 gcaccattct ggtcgtcgcc ccccgccagg caaactacca ctttcagtac gccaacggca
     1861 ccctgacaaa tacaggaaat gcgacgctgc ggatcctcgc ctacggcccc tgcctgaaag
     1921 ccgccaatgg taaagagtgt aaagagaatt actacctgat gccgggcaag tcgcgtcgtt
     1981 ttacccgcgt ggacacggcg gataacaaag gacgggttgc actttggcag ggtgataagt
     2041 tcattcccgt gaaatagata gctgtgcaga tggataacga caatgccttt acgacggttc
     2101 tccccaggac tgaaagccca gtttgccttc ggcatggtct ttttgttcgt tcagcccgat
     2161 gccagcgctg ctgacataag tgcgcagcaa ataggtgggg tgattattcc gcaggccttc
     2221 agtcaggcgc ttcaggacgg catgagcgtc ccgctctata ttcatctcgc cggtagccag
     2281 ggtcgccagg acgatcagcg aatcggcagc gcttttatct ggttggatga tggacagcta
     2341 cgcatccgga aaatacagct ggaagagagt gaagataacg ccagtgtcag cgaacaaact
     2401 cgacagcagc tgatggctct ggcgaacgcc ccgttcaatg aggcccttac catccccctg
     2461 actgacaacg cgcagctgga tctcagcttg cgccaactgc tgctgcagct ggtggtcaag
     2521 cgcgaagcgc tgggcactgt actacgctca cgtagcgaag acatcgggca gtccagtgtt
     2581 aacaccctca gcagtaatct gagctataac ttcggcatct ataacaacca gttgcgtaac
     2641 ggcgggagca acacatccag ctatctgtcg ctgaataacg ttactgcact gcgcgaacat
     2701 catgtggtgc tcgacggctc gctgtacggg atcggtagcg gtcaacagga cagtgaatta
     2761 tataaagcga tgtatgaacg cgattttgcc ggtcaccgat ttgccggtgg aacgctcgac
     2821 acctggaact tgcagtcctt agggccgatg accgccattt cagcagggaa gatttacggc
     2881 ctttcctggg gaaaccaggc cagctccacc atcttcgaca gcagccagtc agccacgcca
     2941 gtgatcgcct ttttaccggc ggcgggtgaa gtacatctca cccgtgatgg gcggttacta
     3001 agcgttcaga acttcaccat gggcaatcat gaagtggata cccggggtct accatacggt
     3061 atttacgatg tggaagttga ggtgatcgtt aacggtcgcg tgatcagcaa acgcacccag
     3121 cgggtcaata agctgtttag ccgggggcgc ggcgtcggtg caccactggc gtggcaggta
     3181 tggggcggta gctttcatat ggatcgctgg tcggaaaacg ggaaaaagac gcgaccagct
     3241 aaagagagtt ggctggcagg tgcctcgacc tccggctcac tgagtacgct tagctgggcg
     3301 gcaacgggat atggatacga taatcaggcg gtgggtgaaa cccgtctgac gctgccgctt
     3361 gggggagcga tcaacgttaa cctgcaaaat atgctggcca gtgacagctc atggagcagc
     3421 atcggcagca tcagcgccac tctaccggga ggctttagtt cgctgtgggt taatcaggaa
     3481 aaaaaccgca ttggcaatca attgcgacgt agcgatgccg acaaccgtgc tatcggcggc
     3541 acactcaacc tgaactcact gtggtcgaag ctgggcacat tcagcatcag ctacaatgat
     3601 gaccgccgtt acaacagcca ttattacacg gcagattact atcaaaatgt ctacagcggt
     3661 acctttggtt cgcttggcct gcgggccggt attcagcgct ataacaacgg cgacagcaac
     3721 gccaatacag ggaaatatat cgctctcgat ctctcgctac cactgggcaa ctggtttagc
     3781 gcagggatga ctcatcaaaa cggctacacc atggcaaacc tgtcagcacg caagcagttt
     3841 gatgaaggga ccattcgcac tgttggtgcc aatctgtcac gagccatctc cggcgatacc
     3901 ggtgatgaca aaactctcag tggtggggcg tatgcacagt tcgacgctcg ctacgccagc
     3961 ggaacgctga acgtcaatag cgcggcggac ggctacgtca ataccaattt aaccgccaat
     4021 ggcagcgtcg gctggcaggg taaaaacatt gctgccagcg ggcggactga tggcaacgct
     4081 ggggtgatat tcaacaccgg gctggaggac gacggtcaga tcagcgccaa aatcaacggg
     4141 cggattttcc cgcttaacgg caagcgtaac tatctcccgc tctctcccta tggaagatat
     4201 gaggtggagt tacagaacag caaaaactca ctcgacagtt acgatatcgt cagcggtcgc
     4261 aaaagtcatc tgactctcta tccaggcaat gtcgctgtca ttgagccaga ggtgaagcag
     4321 atggttaccg tctccggtcg tatccgtgcg gaagacggca cactgctggc taacgcacgg
     4381 attaacaacc atatcggccg aacccgaacc gatgaaaacg gcgagtttgt catggacgtg
     4441 gataagaaat accccactat cgattttcgc tacagtggca ataaaacctg cgaagtggca
     4501 ctggaactca accaggcgcg cggtgccgtc tgggtcggtg atgtggtctg cagcggcctc
     4561 tcatcgtggg cggcggtgac gcagacagga gaagagaatg agagttaacc tactgatagc
     4621 gatgataatc tttgcgctaa tctggccagt aactgcgctc agagcggcag tgagcaaaac
     4681 aacctgggcg gatgcaccgg cacgcgagtt tgtgtttgtc gaaaacaact cagacgacaa
     4741 ctttttcgtc actcctggcg gggcgctgga tccgcgcctg accggtgcca accgctggac
     4801 cggtttaaaa tacaatggtt caggaaccat ctatcagcaa agcctcggct acattgataa
     4861 cggttacaac accggccttt ataccaactg gaagtttgat atgtggctgg aaaattcacc
     4921 agtttcatct cctttaactg gcttgcgctg catcaactgg tacgctgggt gtaatatgac
     4981 caccagtctt atcctgccgc aaaccaccga caccagtgga ttttatggcg cgacggtgac
     5041 cagcggcggc gcgaagtgga tgcacggcat gttgtcagac gcgttttacc agtatctgca
     5101 acaaatgccc gtcggcagca gctttacaat gaccatcaat gcctgccaga cctctgtgaa
     5161 ctatgacgcc agcagcggcg cacgctgtaa ggatcaggcc tccggcaact ggtatgttcg
     5221 caacgtcacc catacgaaag cagcaaatct acggttgata aatacccact cgctggcgga
     5281 agtatttatc aacagcgacg gagtaccgac tctgggcgaa gggaacgccg actgccggac
     5341 gcaaaccatc ggcagccgtt caggattaag ttgtaagatg gttaactata ccctgcaaac
     5401 aaacggactc agcaacacct caatccatat attcccggcg atcgccaact cgtcgttagc
     5461 ctcggccgtc ggggcgtacg atatgcagtt cagtctgaat ggcagttcat ggaaaccggt
     5521 gagcaatact gcctattact acaccttcaa cgagatgaag agcgcagact cgatctatgt
     5581 tttcttctcg agcaacttct ttaagcagat ggtgaaccag ggaatcagcg atatcaacac
     5641 caaagatcta ttcaactttc gctttcagaa caccacatca ccggagtctg gctggtatga
     5701 attttctacc tccaacacgc tgattatcaa accccgtgat ttcagcatca gtattatctc
     5761 cgatgaatat actcagacac cgtcgcggga gggatatgtc ggcagcggcg agtcggcact
     5821 cgatttcggc tatatcgtaa ccaccagcgg taaaacagct gccgacgaag tgctgatcaa
     5881 ggtgaccgga cccgcgcagg tgattggcgg gcgctcctat tgtgtcttca gctccgatga
     5941 cggtaaggcg aaagtaccgt tcccggcgac gctttccttt attacccgca acggagctac
     6001 aaaaacctac gatgccgggt gcgatgatag ctggcgggat atgaccgatg cgctgtggtt
     6061 gaccacaccg tggactgata tctctggcga agtggggcag atggataaga ccacagtcaa
     6121 attttcgatt ccaatggata acgccatttc tctgcgtacg gtagatgata acggctggtt
     6181 tggcgaagtc agcgcttcag gagaaattca tgttcaggcg acgtggcgta acattaacta
     6241 aggccctgct gacagcggtc tgtatgctgg cggcaccttt gacacaggcg atttcggtcg
     6301 gcaatctgac tttttcactg ccgtccgaga ctgactttgt cagcaaacgt gtagtgaata
     6361 acaacaaaag cgcgcggata taccgtattg ccatcagtgc tatcgatagc ccgggcagca
     6421 gtgaattacg cacccgaccg gtggatggtg aactgctttt cgccccccgc cagctggcgt
     6481 tgcaggctgg tgagagcgag tattttaaat tttactatca tgggccacag gataaccgcg
     6541 agcgctacta ccgggtctca tttcgcgagg tccccactcg taacctgaca aaacgcagcc
     6601 ctaccggcgg tgaggttagc acggagccgg tggtggtgat ggataccatt ctggtagtac
     6661 gaccacgtca ggttcagttt aaatggtcct tcgatcaggt gaccggaacg gtaagtaaca
     6721 ccggcaacac gtggtttaag ctactgatta aaccaggatg tgattcgacc gaagaggaag
     6781 gcgatgcctg gtatctacgt ccggaagacg tggttcatca gcctgagtta cgtcagccgg
     6841 ggaatcacta tctggtctat aacgacaaat tcattaagat tagcgattcc tgtccggcta
     6901 agcccccttc ggcggactga tacttatcca tcggtcgagg gaagaattcc acctcagaga
     6961 ctcaaaaatc gtttaaatga tgtggaagcg atcgtcaata tcacctgtaa cgataggctt
     7021 tctcatatgc acaccacagt gttcaggaga cacggaaagg taccaggcgg cctgctaacg
     7081 agtggtttct tcactaatta ggacgtaaaa tcggtaacgg ctggaaatca ttcaatactc
     7141 acactatcga aagtacacca gccaaccgcg atacgtccct gcatacgacg taccgcagtt
     7201 ctctttacga tttatagctg tactggtgaa ttatgagcaa tctgaatcca tgcatgacgt
     7261 gtggtgcctg ttgtgcattt ttccgcgtct ctttctactg ggccgaagcc gacgatgctg
     7321 gcggcactat tcccgccagg ctcactgaac aaatatcccc ttttcaccga tgcatgagcg
     7381 gaactaatca gaaaaacccc cgatgtattg cccttgcagg aaccccgggc aaaaatgcct
     7441 gctgcacgat atataaaaat cgatcgtcca catgcagaga attcgccatg tctggtgaga
     7501 acggagtcgt caatgaggct tgcaatcgtg caagggctaa atacgggctg acaccgttat
     7561 aaatatacaa taattatttg cactgccccg ccagctgtaa tagcggggct tcgtttttca
     7621 ggggtaacaa accccgatac ttcttctatt tgccagcaac aatgcctctc ttctgtagcg
     7681 ttcttcggat ctacatcatc ctgaagtagc gcgatatact cactgactat ctgaatcaga
     7741 atataaaaag caatgttttt aacctataaa aatggcgctg tatttgcgcc atttttatca
     7801 ttcaatgcat tatctgtttg agcctaaagg gatctcaggg tctggctcat gagtaattct
     7861 gtttcgtaaa tctctgcgaa taatttcaat ggaccagaac cagactaaat gtccaaaaat
     7921 ttcagaaaca ttctcatacc acgggagatc aaacagaggt ggtgtcagtc ccatgagagg
     7981 gaatgaaatc atatgaacaa aaagttgggc taaagcacct gccagtaagc cctgccagag
     8041 tttaattttt ggaaatactt ctgcgaccac acaataaccg acagcaaaca ctatcgaaaa
     8101 gataatgtgc gtaacaccaa cccagttaaa gacatgcccg gcaaaggtat aaacagccgc
     8161 attgggatct gtcagcccca gccagtctcg aagaaaaata tacggaggat tgagaaaatt
     8221 acgcgagcaa tcaatttgcc cagcagccct gattaatgat tccgggccac acgctgcatt
     8281 aaacatatcc accgggctac gtggcggcaa tggaacttca gccccccact tcacaaatgc
     8341 ggaaacaacg ccagcaatca gcccaatgaa tgcagcaaga ccataacgtc tgcggttcgg
     8401 tggagtttgt tcaaatatat tcatatctac cctgcttgta ccattatgtt atacacctct
     8461 ttaggagtat tcataaaaca aggtaaatgt aaagaactgt attgttttgt ataacaagat
     8521 ggttgtctaa tcaccaaaga atataagcac catcatttct ccctattttt atattagaag
     8581 tgacatagat ttgcagggtg atgcagagct gacaatcaca ggtttcctta tcggtttttg
     8641 catcgtacaa ctaaagcaat aaaccagctc ccatcatcat caacatcccg gcgaacatga
     8701 atttattagc cgggctcata gctgcattcg cgttttaaga ataatcctcc tgctgtcgcc
     8761 gactatgctt aacgtttaaa aaagcatcag cactctcgca acgcactctt attttcccct
     8821 ttagaatacc ggaggcctgg tatgagcaac caaggcgaat accccgaaga taatcgggtt
     8881 gggaagcatg agccgcacga tttaagtttg acccgtcgcg atctgattaa agtgagcgcc
     8941 gcaacggcgg cggccgccgt ggtttatcct cattctacgc ttgcggcaag cgttccggca
     9001 gctacacccg cgcctgagat aatgcccctg acacttaagg tgaacggcaa aaccgagcag
     9061 cttgaggtgg atacccgaac cacgctactg gacgctttgc gtgaaaatct gcatttgatc
     9121 ggtaccaaga aaggttgcga tcacggacag tgcggagcct gtaccgtgct ggtcaatggt
     9181 cgcaggctta atgcctgcct gacgcttgca gtcatgcatc agggggccga gatcaccacc
     9241 attgaaggcc tgggttcacc agataatctt caccccatgc aggcggcctt tatcaagcat
     9301 gatggcttcc agtgcggcta ctgcacctcc gggcaaattt gctcatcagt agcggtgcta
     9361 aaagagattc aggacggcat tcccagtcac gtcacggtcg atttggtttc ccctccagaa
     9421 agaactgccg atgagatccg tgaacgtatg agcggcaaca tctgtcgctg tggtgcatac
     9481 gctaacatcc ttgccgccat tgaagacgct gcgggggaga taaaatcatg aaggcgttta
     9541 cctatgaacg agtgaacacc cccgccgagg cggcacttag cgctcagcgc gtacccggcg
     9601 caaaatttat cgcgggcggg accaatctgc tggacctgat gaagctggaa attgaaacgc
     9661 ccacccacct tatcgatgtg aacgggctcg ggctcgataa gatcgaagtg accgacgcgg
     9721 gtgggctgcg catcggcgca ctggtacgga acaccgacct ggtggctcac gagcgcgtgc
     9781 gtcgtgatta cgcggtactc tcccgcgccc tgctcgctgg cgcgtctggt cagttacgca
     9841 atcaggcaac cacagcaggt aatctgctcc agcgcacgcg ctgcccctat ttttacgaca
     9901 ccaatcagcc ctgcaataag cgcctgcccg ggagcggctg cgcggcgctt gaaggcttta
     9961 gccgtcagca cgcggtggta ggcgtaagcg aagcctgcat tgccacccat ccgagcgata
    10021 tggcggtcgc aatacggttg ctggatgcgg tggtggaaac catcacgccg gagggaaaga
    10081 ctcgcagtat cacactggct gatttttatc acccaccggg gaaaacgccg cacattgaaa
    10141 ccgccctgct tcccggtgag cttatcgttg cggtgacgtt acctccgccg ctcggcggaa
    10201 aacatatcta ccgtaaggtg cgcgatcgcg cctcctacac ctttgccctg gtatcggtcg
    10261 cggcgattat tcagcctgac ggcagcgggc gcgtcgcgct gggcggagta gcacataagc
    10321 cctggcgcat tgaggctgcg gatgctcagc tatcccaggg ggcgcaggcc gtatatgacg
    10381 cgctgttcgc cagcgcccat cccaccgctg aaaacacctt taaactcctg ttggcgaagc
    10441 gaacgcttgc ctccgtactg gctgaagcga gggcacaagc atgaaatttg ataaacccgc
    10501 aggggaaaac ccgatcgatc agctgaaggt tgtcggtcgt ccccatgacc gcatcgacgg
    10561 accgctgaaa actaccggca cggcacgcta cgcctacgaa tggcatgaag aatcccccaa
    10621 cgccgcctat ggctatatcg tcggttccgc cattgccaaa ggacgcctca ccgcccttga
    10681 tacggacgcc gcgcaaaaag cgccgggcgt actgcctgtc attaccgcca gtaacgccgg
    10741 ggcactcagt aaaggcgaca aaaacaccgc caggctgtta ggcggcccca ctattgagca
    10801 ctatcatcag gccattgctc tggtagtggc cgagaccttc gaacaggcgc gagcggcggc
    10861 ctcgctggtg caggcgcact atcgccgtaa taaaggagct tactccctgg cggacgaaaa
    10921 acaggccgtc agtcagccgc cggaagacac gcccgacaag aacgtcggtg actttgacgg
    10981 ggctttctcc tccgctgcgg tgaagattga tgctacctac acgaccccgg accagagcca
    11041 tatggcgatg gagccgcatg cctcgatggc cgtctgggat ggaaataagc ttactctctg
    11101 gacctcaaat cagatgattg actggtgccg caccgatctg gcaaaaacgc tgaaagtgcc
    11161 cgtggagaat gtgcgtatta tctccccgta tatcggcgga gggtttggcg gcaagctgtt
    11221 cctgagaagc gatgcgctgc tggcggccct cgccgcccga gcggtgaaac gtccggttaa
    11281 agtgatgctc ccccgcccct ctattcccaa taacaccacg caccgccccg ccacccttca
    11341 gcacttgcgt atcggtgccg accagagcgg gaaaatcacc gctatctcac atgaaagctg
    11401 gtctggaaac ctgcccggcg gcacgccgga aacggcggta cagcaaagcg aattactcta
    11461 cgccggggca aaccgtcata ccggcctgcg gctcgccacg cttgatttgc cggaagggaa
    11521 cgccatgcgt gcgcccggcg aagcccccgg tctgatggcg ctcgaaatcg cgatcgacga
    11581 actggcggaa aaagcgggca tcgatcccgt cgagtttcgc atcctgaatg acactcagat
    11641 tgaccccgcc gacccgacgc gccgcttctc tcgccgtcag cttatcgagt gcttgcgcac
    11701 cggagcggat aaatttggtt ggaagcagcg caacgccaca cccggacagg tgcgcgacgg
    11761 ggagtggcta gtcggccacg gcgtcgcggc gggctttcgc aataatctgc tggaaaaatc
    11821 gggggctcgg gttcacctcg aaccaaacgg caccgttacc gtggaaacgg acatgaccga
    11881 cattggcacc ggcagctaca ccattctggc ccagacggca gcggaaatgc ttggcgtacc
    11941 gctggagcag gttgcggttc acctcggcga ttccagtttc ccggtttctg cgggttctgg
    12001 tggacaatgg ggcgcgaata cctccacctc cggcgtttac gccgcctgtg tgaagcttcg
    12061 cgaaatgatt gcctcggcag tcgggtttga tcctgagcag tcgcagtttg ccgacggcaa
    12121 gattaccaac ggtacccgaa gcgccatact acatgaggcc accgcaggcg gcagactgac
    12181 agcggaagag agcattgaat tcggaacact gagcaaggag taccagcagt cgacctttgc
    12241 cgggcatttt gtggaggtcg gcgtgcatag cgcgacggga gaagttcggg tccggcgtat
    12301 gctcgctgtg tgtgctgcag gacgcatcct gaatccgaaa actgcacgca gccaagtcat
    12361 tggcgcaatg actatgggca tgggcgcggc actgatggag gagctggcgg tggatgaccg
    12421 tttgggctac ttcgttaatc acgatatggc ggggtatgag gtgccggtcc atgcggatat
    12481 cccaaaacag gaagtgattt tcctggatga taccgacccc atatcctccc cgatgaaggc
    12541 caaaggtgtc ggtgagctgg gcctgtgcgg cgtgagcgcg gctatcgcca acgcggtgta
    12601 taacgccacc ggtattcggg tacgcgatta tcccatcact ctggataagc tgctcgataa
    12661 gctgccggat gtggtttaag gaggaacaat gtcatacccg ctttttgata aagacgaaca
    12721 ctggcataag ccagagcagg cgtttctcac cgatgaccac cggaccattc tgcgcttcgc
    12781 cgtagaggcg ctaatgtccg gtaaaggagc ggtgctggtg acgctggtgg agatacgcgg
    12841 cggcgcggcg cgcccgctcg gggcgcagat ggtggtgcgc gaagatggtc gttactgcgg
    12901 ttttgtctct ggcggctgcg tggaggccgc tgccgctttt gaagcgctgg agatgatggg
    12961 ctcaggctgc gatcgcgaaa ttcgctatgg cgaaggttcg ccgtggtttg acatcgttct
    13021 gccctgcggc ggtgggatca cgctgacgct ccataaacta cgctcggcac agcctctgct
    13081 cgccgtgctg aaccgcctgg aacagagaaa accggcgggg ctgcgctacg atccgcaagc
    13141 acaatcgttg gtgtgcctgc ccacgcaaac ccgaacgggc tggaatctca atggctttga
    13201 ggtggggttc aggccatgcg tcaggctgat gatttacgga cgttctcttg aggcgcaggc
    13261 aaccgcgagt cttgcagcag ccacaggcta tgacagccat atcttcgatc tttttccggc
    13321 ctcagccagc gctcagatcg ataccgatac ggcggtcatt ttgctgtgcc atgatctcaa
    13381 ccgggagctg ccagtgttgc aggccgcgcg agaagcaaaa cccttttatc tcggcgcatt
    13441 gggcagctat cgaacccaca cgttacgtct gcaaaagctc cacgagctgg gatggtccag
    13501 ggaggagaca gcgcaaatcc gggcacccgt cgggatattc cccaaagccc ggaatgcgca
    13561 tacactggcc ctctccgtgc tggcagaagt cgcctctgta cgtctccatc aggaggagga
    13621 ttcatgcctg cccccgtcgt cctgatcctt gcggccgggc gtggaaagcg ctttctcacc
    13681 tccgggggaa ataccataaa ttaagagaaa aatttgcagt agccgtttta gaaaatcacc
    13741 taaatcgttt aaaagagcgc cctacccata gtggttgctc atcgccttgt gtaacttttt
    13801 cagcaacgcg cgccctcgcc ggactaaagc gcaccggcct tctggcgtgc tgtaacaaag
    13861 gtcagtacgc ccgcgcaaag gagcagcaca ctactcatgg caaacgtact ctgccagcca
    13921 aggcggtcaa acacgatgcc tcctgccgtt gatccaaggg cgattgagag ctgaatcacc
    13981 gcgaccatga gcccgcctcc ggcttcggcg ttgtcaggca acgtacgcgc aatccatgtc
    14041 caccatccgg ttggcgcggc ggtggccagc atgccccaga gtcctaacag cagcgagacc
    14101 atccagacgt gatgcccggt aagcatcaga ccgatggcaa tgacggccat cagtccgggt
    14161 atggccatca gcgtggggta gaattttctg ttgagaaata tcgaaacaac cagcgtgcca
    14221 ataaaaccgg ccacgccgat aatcagcaaa atcaacgaca agcctaagga attaacccgt
    14281 gtcaccgact ccagaaatgg gcgcacatag gtgaatagcg caaactgccc catgaaaaac
    14341 aggccacaag ctatcatgcc gaccacaacc atgcggcggc taaataagcg gaataccgcg
    14401 ccacatgacg tgtggctttt attcgcatcc atgctgggca gactaatgca ctgccagata
    14461 aaggcgacaa ccgccatggg caccaagcac aagaaagcgc ctcgccatcc gaccgtagcc
    14521 cccagatagc ttcccagcgg cgcggccacg accgtcgcca gcgcattacc ggcgttgaaa
    14581 atcgccagcg cacgcgtgac ctgatgctgc ggcaccagac gaatcgccgt tgccgctgac
    14641 atcgaccaga atccgccaat ggccacacca atcatcgcac gaccgaccat atacatcaga
    14701 taactggtag ccagcgcaat aataagcccc gatacggcca tcagaaccgt catccccagc
    14761 agcaggaact tacgattcat ctttccagct agcgttgaaa gcgtcaggct ggtcagaacc
    14821 gccagcgcac cggagatagc aatcccccgc cctgccagcc cctccgtcac gcccaggtca
    14881 cgggcaatcg gcgtgagcag gctgacgggc ataaattcag aagcaatcag cacaaacacg
    14941 cacagggtca tggcaaaaat accgccccag tatgcgtgct gatgatgagg tgatggtgtt
    15001 tggcttagtg tcgacatagt gaaatttcac aaagatcagg ataaaagcca ccggccatag
    15061 accggtggct ttgcatcaat tcaggggagg ttttatttca gtttggtctg gaaaaattgt
    15121 tcgaacttcg cgaaaggtat ttttccggcc acgttatcgt acaggtccac gtgatttgcc
    15181 cctggtacca ccaccaactc tttgtcttta ctgccaaccg ccttgtaagc atcttcagca
    15241 aaatagcgtg aatgtgcttt ctcaccggtc acaatcagcg tcggaatggt gatttcactg
    15301 gcgtagctca acagcggcat attcataaat gacagcggca tcgttgccgt ccacgccccg
    15361 gtagagttaa cggaacgttc gtggaagccg cgcggcatgc gatagtagtc aaagaactct
    15421 ttcagtaccg gatgaggatt tgccggcagc gtttctggca gaatgcgctc gcccgcactg
    15481 accttgccgt tgctatcgac attaatatca tgaccaccgt gagcgaacgt tccgctctgc
    15541 gcatccttcc agcgctgttc attcaaatat tgcagaacag cacgacggtc ggcggtagaa
    15601 taacggtctt cgccatcccc cacaccatgg cccatcgccc ggctcatgtc atacatcacg
    15661 ctggtagcaa ccgctttaac gcgggtatcc atcgccgcgt cgttcagcgc catgccgccc
    15721 cagccacaaa taccgagcag cccgatacgg ttgcgatcta cctctttttg cagccctaag
    15781 aaatccaccg ccgcgctgaa atcttcagtg ttgatatccg gtgaggcgac atttcgcgga
    15841 tagccgccgc tttccccggt gtaagaagga tcgaacgcca gggtaacaaa ccctcgttcc
    15901 gccagcgtct gcgcatacaa gccgctggat tgctctttca ccgcgccaaa tggcccactg
    15961 accgcaatcg ccgccagctt gcgatcgcca cggtctttag gcaggtaaag atcgcccact
    16021 aaggtgatgc catatcggtt cgggaacgag actttacggt gatcgacttt ctggctctcg
    16081 gcgaacgttt tatcccattt atcggtcatg gacacagggg cattaggatt ggttgaatca
    16141 gcataactca ttgtcgtgac tccacttaat gatgcgaaaa gcagcatgac cggcatcgcg
    16201 gtttttagcg ttcgggtgaa ggctttcata aaaatcccca taaaaaacaa cagagagata
    16261 caggtttccg acggacaacg ctgcggtgct ttactttgat ggggtcatta tagtgagcag
    16321 aattagtgct gattaggggg cgattaatgc taagattaat accatattgt tataaattaa
    16381 tgctgagatt gccggcatga aggggaatgt ttcaatggca aaaagggaga actacaacga
    16441 gctatacctg tttatgcagg tggtgcggga aggcagcttt accgcagcgg ctaagcggct
    16501 gggtcttgct cagtcaggag tcagccgttc tgttcgcgag cttgaagaaa ggctgggcgt
    16561 ccagctgctg gtgcgcacca cgcgtaaact gtcgctgacg caagcaggcg agcagctcta
    16621 ccagaagacg gcatctggat ttgaaatgtt agatcttggg cttgccacgc tggcgcacta
    16681 tcgagagacg ccctccggca cggtgcgtat caatgccagc cagcacgcga ttgataaatg
    16741 cctgctgcca aagctggcgg tatttaaaca gcgatatcct gatatccagc ttgaactgat
    16801 aaatgagagc cggttcgtcg atatcatcga ggaaagattc gatgcaggtg tccgcctggg
    16861 gcctgaagtc agccagggca tggtcgcggt acgcatcacg cccgatatgg agatggcaat
    16921 tgtggggact ccggaacatt ttcgtcgcta tggttttccg caaaccccgg cggatttaaa
    16981 ggcgcatccc tgtatcgcct atcagtttgc cgacggcagc gtataccagt gggaactcaa
    17041 tcaggatgat aaaaaaatca ctcatcaacc cgaaggacaa tgggcctttt ccgacagcta
    17101 tatggaagca gaagccgccc ggctgggcct cgggttggcc tatgttcctg ttgaactggt
    17161 cgcggatgat ttagaacacg gtaagcttat cagagtgttg cagcgttaca gcctgcgaat
    17221 ggagggattg tttctctatt accctcatcg caacgtctcc cccgctctgc ggatggtcat
    17281 tgatacattg aaaatctgaa cgttaacaac gaccgactct tcactgtgca atattgtgtc
    17341 aggtagtcac tctgcgttca attccagggg ccgctttgag cgagaaagag cggacttatc
    17401 tgtacaaggg gactatccag caatggagat catccaaaag aaaaaaccac ctggctcccc
    17461 tcgacaacct taatccgggc atttagcctc aatcaacatt ttcatatctg tcaggttact
    17521 tcattatgaa aaccgatcaa taccgttgtt tttgttgcaa cagtagaata cgcgtattta
    17581 tccctccatt tctattaata acggcaataa actgttcatt tcactgatat ttaaaatatg
    17641 catccttttc aagctgttca gcggcgtaag aaaacgtatt aaatatgaat tcgacgattt
    17701 aaccatttct gtctgattgg taatcatctg tgattaatgc tggtctattg attactccag
    17761 cattaaccgc ttagggatgt attcttcctg ttgatataca gccctcgaaa aagagagaaa
    17821 ataaaactgt gtccaatatt actctaccac tccagaccga agcatcggta gaattagcag
    17881 aacgactggt acaatagatt gggatagcgt catttgtcag gtattccatt gcctgacaaa
    17941 tgacaatttt gagtcaatag acgtgcttct tgatcatgtc ttaaaaatta taccatgagc
    18001 cttatgaagc atcattttta ctgactggtg ttaactttgc tatcatataa actctgttct
    18061 taaaaaacgc gcccagaatt ttatatgatt tcccttgttt ttctgcctgt aaagaaattt
    18121 ctttttcagt gctgttaatg gttgaaccga cggcagtaat cgttttggcg acgctactga
    18181 atgataataa agacagtgca accacaacaa ttaaagaaca gatagtattc acgctaattt
    18241 ccctgacaat tgagaggatg agttttatat ataaagacat taaacatgag tcatgtttac
    18301 ttcctgtgaa aaataatctt tctgtaatta aaccttcatc tccttc
//
LOCUS       DAZFTN010000090        18180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFTN010000090 DAZFTN010000000
VERSION     DAZFTN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(14..592)
                     /gene="mocA"
                     /locus_tag="ACHKV2_003533"
     CDS             complement(14..592)
                     /gene="mocA"
                     /locus_tag="ACHKV2_003533"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO6941356.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            695..1465
                     /gene="yqeC"
                     /locus_tag="ACHKV2_003534"
     CDS             695..1465
                     /gene="yqeC"
                     /locus_tag="ACHKV2_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO6941357.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1513..3138
                     /gene="yqeB"
                     /locus_tag="ACHKV2_003535"
     CDS             1513..3138
                     /gene="yqeB"
                     /locus_tag="ACHKV2_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO6941358.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3179..4111)
                     /gene="arcC"
                     /locus_tag="ACHKV2_003536"
     CDS             complement(3179..4111)
                     /gene="arcC"
                     /locus_tag="ACHKV2_003536"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6941359.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4159..5544)
                     /gene="hyuA"
                     /locus_tag="ACHKV2_003537"
     CDS             complement(4159..5544)
                     /gene="hyuA"
                     /locus_tag="ACHKV2_003537"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO6941360.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5597..6808)
                     /locus_tag="ACHKV2_003538"
     CDS             complement(5597..6808)
                     /locus_tag="ACHKV2_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO6941361.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6866..8062)
                     /gene="dpaL"
                     /locus_tag="ACHKV2_003539"
     CDS             complement(6866..8062)
                     /gene="dpaL"
                     /locus_tag="ACHKV2_003539"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO6941362.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8120..9307)
                     /gene="ygeW"
                     /locus_tag="ACHKV2_003540"
     CDS             complement(8120..9307)
                     /gene="ygeW"
                     /locus_tag="ACHKV2_003540"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO6941363.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9786..11564
                     /locus_tag="ACHKV2_003541"
     CDS             9786..11564
                     /locus_tag="ACHKV2_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6941364.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(11604..12083)
                     /gene="xdhC"
                     /locus_tag="ACHKV2_003542"
     CDS             complement(11604..12083)
                     /gene="xdhC"
                     /locus_tag="ACHKV2_003542"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO6941365.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12080..12958)
                     /gene="xdhB"
                     /locus_tag="ACHKV2_003543"
     CDS             complement(12080..12958)
                     /gene="xdhB"
                     /locus_tag="ACHKV2_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO6941366.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12969..15266)
                     /gene="xdhA"
                     /locus_tag="ACHKV2_003544"
     CDS             complement(12969..15266)
                     /gene="xdhA"
                     /locus_tag="ACHKV2_003544"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO6941367.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKTFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15682..16437
                     /gene="actS"
                     /locus_tag="ACHKV2_003545"
     CDS             15682..16437
                     /gene="actS"
                     /locus_tag="ACHKV2_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO6941368.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16516..16589
                     /locus_tag="ACHKV2_003546"
     tRNA            16516..16589
                     /locus_tag="ACHKV2_003546"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16548..16550,aa:Gly,seq:ccc)
     gene            16823..18180
                     /locus_tag="ACHKV2_003547"
                     /pseudo
     CDS             16823..18180
                     /locus_tag="ACHKV2_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4514 a         4316 c         4625 g         4725 t
ORIGIN      
        1 ttaactacgt tttttaaatt ttagtatacc ttttttgcgc tgtaataaag tcgtccggag
       61 tatcaatatc taaaattatt tctgcatttt ctacttcgac ggaataatga gctcccataa
      121 ggagtgcctg acgcatattg gtgatattgg ggcgcttgat tgcctgcatc aggcatggtt
      181 ttgacactaa aatcggatgg ccggggatgc cattatggag tggcagtatt gcgccatcat
      241 ttcgtaacga ccagattttt ctgaaaatat cgatggtgag ggttggcatg tcaccgtggg
      301 tgagaaaaca atgttctgtt tgcactgcgg gtactgcggc ctttactgat gttagtaaac
      361 cctgcgcata atctgggttg tggataatag tgatattgct ctggttcgca taacgttcgt
      421 gcagttcatt accacgatag ccggtgacta aaataattcg gctacaaaac tgcaacgcat
      481 ttttgatact tgtatcaaga attgtcccct gttgccaggg taacatcatt ttccattgcc
      541 ccattcttga tgataatccg gcagcggtaa ttatacagtc gatggctgac atagtggttc
      601 cattatttat aggggctaat aacgcctgat aattgcgtta cttcctgatg gattaagttg
      661 gggataattt atcccagaga ggtcataaag actcgtgaaa agtatagttg acccatcggc
      721 attattcatt gatttaggtg cgcagaaacg tcccactgtg atttctgttg ttggtgctgg
      781 ggggaaaacc agcttgcttt tttggctggc agaactgctc caggctagcg gcaggcgtgt
      841 gttaatcacg acgacaacac atatgtttat gccaacgtct cactggcccg tggttttctg
      901 tcgtgatccc gccatgcttc ctcatgcgtc tcttacatcc cccatttcat tttgttttca
      961 cagctggaaa gcgaacctgg gaaaagtgca gggatttacg ccagaagcga ttgatgcact
     1021 ggtgcaacga ccagagtgtg acgtaattct cattgaagca gatggctcgc gtgcaatgcc
     1081 gttaaaagcg cctgatgagc acgaaccttg catacccaaa agcagttgct gcgtgattgc
     1141 tgtgatggga ggacatattt tgggcgcgaa agtgagcacg gaaaatgtcc atcgctggtc
     1201 gcagtttgct gatattaccg ggttaacacc tgatgcaacc ttgcaactga gcgatctcgt
     1261 tgcgctggtt cgccaccctc agggggcgtt taaaaacgta ccgcaaggtt gtcggcgagt
     1321 ctggttcatt aaccgttttt ctcaatgtga gaatgcgatt gcgcaaagcg agctccttca
     1381 accgctgcaa caacacgacg tagaggcaat ctggctgggc gatatacaag aacatcctgc
     1441 aatcgcgcgc agatttgtga attagcggcc cgggtattcc gggttactga aaggatatcc
     1501 attgaggttg gtatgaatat tttcacagag gctgcaaaac tcgaagagca aaattgtccg
     1561 tttgctatgg cgcaaattat tgatagccga ggctcgactc cccgccattc tgcacaaatg
     1621 ttagtgcgcg ccgatggctc tatcgtcggt acaattggtg gcggaatggt tgagcggaag
     1681 gtgattgaag agtcgcttca ggcattgcag gaacgtaagc cgcgattatt ccatggacgt
     1741 atggctcgta acggtgcgga tgctgtcggg tcagattgtg gaggtgcgat gtcagtgttt
     1801 atcagcgtcc atggtatgcg tccacgtctg gtgttgattg gcgcggggca tgtcaaccgg
     1861 gcgatagccc agagtgcggc gctattagga tttgatattg ccgttgccga tatttatcgc
     1921 gaaagcctca atcctgaact attccctcca tcaaccacgc ttctccatgc tgagtcgttt
     1981 ggtgcggcag tggaagcact ggatattcgc cctgataatt ttgtcctgat tgccacgaat
     2041 aatcaggatc gtgaagccct cgacaaactc attgaacagc ccattgcatg gttggggttg
     2101 ctggcaagtc gtcgcaaggt tcagcttttc ctgcgtcaat tgcgtgagaa aggcgtggct
     2161 gaagaacata ttgcccgttt acatgcgccc gttggttaca acataggtgc ggaaacgccg
     2221 caggagatcg ccatcagcgt gctggcagaa atattacagg tgaaaaataa cgcgccgggt
     2281 gggctgatga tgaaaccttc tcatccttcc ggacaccagc tggtggtgat tcgcggtgcg
     2341 ggagatatcg ccagtggtgt ggcgctacgt ctgtatcatg cgggttttaa agtgatcatg
     2401 ctggaagtgg aaaaaccgac agtgattcgt tgtaccgtgg cgtttgccca ggccgtgttc
     2461 gatggcgaaa tgacggtcga aggcgtcact gctcgcctgg caaccagctc tgcggaagcg
     2521 atgaaactta ccgaacgcgg attcatccct gtgatggtag atcccgcctg ttcattgctt
     2581 gatgaactga aaccgctttg cgtggtggac gctattctgg cgaaacagaa tttgggaaca
     2641 cgggcagata tggcaccagt aacaatcgcg cttgggccgg gctttgctgc agggaaggat
     2701 tgtcatgcgg taattgaaac aaatcgcggg cactggctcg gtcaggtgat ttactgtggt
     2761 tgtgcgcagg agaataccgg tgttcctggc aatattatgg ggcataccac ccgacgggtg
     2821 atccgtgctc ctgctgcagg cattatgcga tccaacgtga aattaggcga tctggtgaaa
     2881 gagggcgatg tgattgcctg gattggtgag catgaaatta aagcaccgtt gacggggatg
     2941 gtgcgtggct tgttgaacga cggcctggca gtggttggtg gttttaaaat tggtgatatc
     3001 gatcctcgtg gtgaaaccgc tgatttcacc agcgtttctg ataaagcccg ggcgattggc
     3061 ggcggcgtac ttgaggcgtt aatgatgttg atgcatcagg gcgtgaaagc gacaaaagaa
     3121 gtgctggaag tggcttaaat gaaaatggcc ggatggtatt acccacccgg ccttaaaatt
     3181 actgacaaat caccgttccg gttttgcctt caatcccttc tttcgctttg ctcaatacgg
     3241 taatcagtgc ttcacgacct gcgcgagagc gagcaaacga ggcggcagct tccactttag
     3301 gcagcataga acctttcgca aaatgacctt cttcgataaa gcgttcagca tcacttaatg
     3361 acaggcgatc gagccactgt tcattctctt taccgaagtt aatggccact ttttctaccg
     3421 cagtcagaat gatcagcata tcggcatcaa tcatttctgc taaacgggcg ctggcccagt
     3481 ctttatcgat aaccgcgctg gcaccacgca gatggttacc ttcacgaata acaggaatac
     3541 caccaccgcc aacggtgatc accacctggc ctgcttctac cagcgctttt accgtctctt
     3601 tttcaatgat atcaaccggt tttggcgagg caacaacacg gcgatagccg cgaccagcat
     3661 cttctttcag agtgtaaccc tgttttgtca gttgctctgc ttcttgctcg gtaaagaacg
     3721 aaccgatcgg cttggtcggg ttgaggaatg ccggatcgtt agcgtcgact tcgacctgag
     3781 taaccagcgt tactacaggt ttattaatgc cccgagaaag cagttcttct cttaaggcgt
     3841 tttgtagatc gtaaccaata taaccctggc ttaaggcaac acagacagac atcggcagca
     3901 ttggcgaatg cgcttcagtt tttgcggcag cttcaaaggc ctggttaatc atgcccacct
     3961 gcggcccgtt accatgagtg acgataactt catgtccctg ggcaattaaa tcaacaattg
     4021 cctgagaagt aatttttacc gctttcattt gtccggccag gtcatcgccc agcgcattcc
     4081 cgcccagggc gagaacaatt ttcttactca tttttcctca ctcatcattc ttgattgtgt
     4141 atagatccct ggcagtggtt agagcacggg agggacaaac ggtttgcgtc gcaggaaacg
     4201 acctcgcccg gctttgcctg taaaggtgcc gtcacagaaa atcgtttcac cacgggataa
     4261 ggttctgaca atcgcgccct gacaggtaaa accctcccat ggcgagtagt cggcgttgtc
     4321 gtggagatgg cgatgctgaa tttgttggct ctgacgtggg tcgataatca ccacgtcgcc
     4381 atcggaaccg ggcgctaata atcctttttg cggccacagg ccaaacagcc tggcgggcat
     4441 tgcgctggtt aattcaacaa agcgttccgg tgttatacgt cccgtcatca cgccactgga
     4501 aaacagtaac tgcatgcggt tttccacacc gggtaagcca tttgggcagc gactgaaatc
     4561 gcctttagaa atttgcaggc gttgagccat cgagaaggtg cagtgatcgg tcgccaccac
     4621 gtcaatcgca ccatcgctga tgccacacca cagtttgtcc tgctcgcgta cgttacgcag
     4681 cggtgggcta agaatgaact tcatgccgtc ttctgtatcg taactgcgtt cgtccaacag
     4741 gagatattgt gggcaggttt caacccagac tggctggtga ttcgcacggg caagacgcag
     4801 ataatccaga cctaagccgt tagacaggtg cacgatatag agcggggcgt taccggcaat
     4861 ttgtgccagg ttaatcatgc gggcgatggc ttccgcttcg cattccagag ggcgactcaa
     4921 ggcatgatag cgcggcgcgg ttaaccctgc ggcgataaat tccgcccgct tgctggcgat
     4981 agccgcatca ttttccgggt gcacggtggt cagcgcgccg gattcatgca gacggcgtaa
     5041 tgcctgcaaa acctcgtcat cgttgagttt gtattgatag gttaaataga gtttaaaact
     5101 gctcagccct tcctcgacca tcatcgggat ttcgtcgagg atggcgtgat taatgtgctg
     5161 gatcacaccg tgaaagctgt aatcgatgac cgctttatgg gcggcataac cacgataaac
     5221 ctccagttga tggcgtaacc gacagccgtt tgggccaaat cccatatggt caataatggt
     5281 tgttgtaccg ccacacgcag ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc
     5341 gatgccgaca tcaatattga aatgcgtatg gacatccacg ccgccaggga aaacgtaaca
     5401 gccagtggca tcaatttctt catacgggag ctgcggcgaa atattgttgc ccaactggcg
     5461 aacaatcccg ctttcaatca gcaaatcctg tttggcttgt ccatctgcgt taacgacagt
     5521 gccgtttttg atcaatacgc gcatagcaaa ctccaaaaac cctccggcga accggagggg
     5581 aataagtgaa gagtaattat tcggttgcca gccagcttaa cgggattgca gcgtacatcg
     5641 cggcacaggt caccaggtga gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt
     5701 ctttacccgg gccaaagccg atcaccggaa tgccgtgacg gcccatgata gatacgccgt
     5761 tagttgagaa ggtccactta tcaacaaccg gcgctttgcc aaacagccct tcgtaggcat
     5821 tcaccagtgc tttaacggtg aagtgatctt cttccacttt ccaggtcggg aagtagcatt
     5881 cggttgggta aaccaggcca gtccaggacg gacggtcgta gttgtacata gaaacaaccg
     5941 cgttagcttt ctgtactgca ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt
     6001 cgccccaggt cagacggcgg tcaatagaga cggcgcagct atctgctaca gcgcaacggc
     6061 ttggggatgt gaagaagatt tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat
     6121 aacccagacg ttgggagagt tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg
     6181 cgttgtcacc gcgttctggc gcagaaccgt ggcagctaac accctgaaca tcaatacgaa
     6241 tttccatacg accgcgctga ccacggtata cctggcagtc ggttggttcg gtactgacca
     6301 caaattccgg gcgaatgccg gattgttcaa taatgtactg ccagcacaga ccgtcgcagt
     6361 cttcttcctg cacagtaccg gtaaccagca gggtatattc atcttccaga ccgaggtctt
     6421 taatgatttt accggcataa accatagatg ccatgccgcc ttcctggtcg gaagtaccgc
     6481 gaccaccgat cagctcatca gtttccatgc cttcgtacgg atcgaagtcc cagtttttga
     6541 tgttgccaat gccgacggta tcgatatgag cgtccattgc caccagacgc gggccgtggc
     6601 cgatataacc gagaacgtta cccatcgggt cgatttcaac tttatcgaag ccgacttttt
     6661 ccatctcttc tttaatacga tgtactaccc gtttctcgtc gcagctttca ctgggaatag
     6721 caaccatgtc gcgcaggaag cgagtcatat ccgcctggta atcttttgct ttttcaagaa
     6781 tcagtttgaa tggaatattc ttagccatta tttttctcca gattttttac tccccgtttt
     6841 gcagggagca gttccaatag ggtgattaag gtgctacagc gtgtttgcct tcccagacaa
     6901 cttcgcggta gtgcttcacg tcggtgtcgc cttcagtgct gataaccagc actacggcat
     6961 ctttgttcag cgccagtttt tccatcaggc tttgacgttg cgggtgataa tgaaccgctg
     7021 cgagaacgcc caaaccgaca gcgccggatt caccggagat gatgcgcggg tcgttgccgt
     7081 acggattacc cagcacgcgc atacctaatg cggcaacgct gtcctggcag gagatgaatt
     7141 gggtggcgca gttacgtagg atttcccagc ccagcgggtt aggttcgcca caggccaggc
     7201 ctgccatgat ggtggccata tcaccgccaa cgttgacgat gtcgcctttg acgccggagc
     7261 gataaataca gtcagctttg tcaggttcaa caataatgct gtgcagattt tgcgggctat
     7321 agacgtcgac cagataaccc agcacaccac cggccattgc tccgacaccg gcttgcagca
     7381 gaacgtgcgt cggggttacg cccatttcac gcatttgctc gacggcttca tctgccaggg
     7441 ttgcgtagcc ttgcatgatc caggttggga ttttggtgta accttcccat gccgtgtcct
     7501 gtaccacttc ccagccgtgc tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat
     7561 agttcatatc tgtgacgatg cactcggcac cgaggttcag aatggcgtca acgcgttcct
     7621 gagcagaacc tttcggcatg taaatcaccg cattctgtcc gagttgctgc gctgcccacg
     7681 ccacaccgcg cccgtggttg ccgtcggtgg tggtcgcgaa agtcattttt tcgccgatgg
     7741 catttttcag gtgctcaaat gacagcgttt cgatatcaag atgatatttt tcacacaata
     7801 attgagcgat agcgtacgca ccgccgagca ttttgaaggc gttcagaccg aatcgttttg
     7861 attcgtcttt gacaagaatt ttcttcaccc caaaaaggtt tgcgagatcg tccagcgcac
     7921 aaagcggtgt tgggcgataa ccagctattt tctggtggaa ctggcgcgcc agtttggcct
     7981 ggctttgcga aaagagcggt gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa
     8041 tcttcaatga gaaaacggac atatcctatc cttttggcct ctggaaatag agggccgacc
     8101 agctgatcgg cccgaggggt tatttcacgc gttcttgcgc ttctttcagc agttgctcga
     8161 gcagtgcacc tggtttggcg tatttacggg acaggatcat cgcggcgatg atataaggct
     8221 tccagctggc ttctttgtag gtagcgatac ggtatttttc gaatacgcct tcagtcactt
     8281 ccccttcttt gcaggatacg ccgctgatat cagctggcag gcagtgcatg tacagggctt
     8341 cgccatcacg ggtcagttcc atcatctctt cagtacaatg ccagtctttg tgttgcgcgt
     8401 tctgtgccag acactgtttt tccagtgctt ttaagccttc gtgatcgtta gcgcgcagca
     8461 attcggtacg ctgttccatc actttgtaag gtgcccatga cttcggataa acgatgtctg
     8521 catctttgaa cgcttcttcc atgctggtga cctgacggaa gctaccgcca gatgctttag
     8581 cattgttttt cgcgacttct actacatccg ggatcaggtc gtagccttcc ggatgggcca
     8641 gggtgacatc cataccgaag cgagtcatca gaccgatgat gccttgtggt acagagagcg
     8701 gtttgccata gcttggagag taggcccagg tcatggcgat ttttttacct ttcaggtttt
     8761 ccagtgaacc aaagtgttca cgtaaccatg ccaggtcagc cattgactga gtcgggtggt
     8821 caatatcgca ttgcaggttc actaaagccg gacgctgtgg cagtacaccc tgcttgtaac
     8881 cgtcatcaag tgcagcgcca acttcacgca tataggcgtt gcctgcgccc agatacatat
     8941 cgtcgcgaat accaatagcg tcggcgcaga aggagatcat attggcggtt tcacgcacgg
     9001 tttcgccgtg agcgatttgt gatttgcctt catcgagatc ttgctgtgca aggccgagca
     9061 ggttaagcgc ggaagcataa gagaagcggg tacgagtgga gttgtcgcgg aatacggaaa
     9121 tacctaatcc actattaaag actttggttg agatgttttc agcacgcagt gcttttaatg
     9181 ctgcggcaac gtccagtact tgtttcagtt catctggagt ctgttcccac gttaacaaaa
     9241 aatctttctc gtgaaggtga gaggtcagcg aattgatatc cttaatcagc tcattaacag
     9301 ttttcatcat aaagtcccgg tagtggaatg acaggcaaag tggtttattt cactgtgact
     9361 tgccaaagtg attggtgttt tcacagctat acctgggact aacaataagt gtgccagaat
     9421 tatccacggg gctgcaaaag actaatttga cggataatgt gtggggttga tcacaaattg
     9481 atgccagctt ccggttgttt gtaaaatacg aaaacgtttg gcttttttta tcgcgttatt
     9541 tatttgctgc taagtatcag aaataagata aagggacagg ctatccctcg tcagtttgat
     9601 aaactcaccg ccataattct catattgata tagaaatgtg atatctcttc cataatttca
     9661 ttagaaagtg ataaaaatct tatgttttta tcaaagccaa tttcgcctga gttaatatga
     9721 tcatgatctg tgaaccatca acgtcttcaa tgatcgactt ttaagtaagt ctggaaggta
     9781 aagctatgga gcttgctact acgcagtcag tattgatgca aattcaaccg acaattcagc
     9841 gttttgccag aatgcttgcc agcgttttgc agcttgaggt tgagatcgtt gatgaaaact
     9901 tgtatcgcgt cgccggaacg ggcgcgtatg ggaagtttct tggtcgccag ttgagcggca
     9961 actcacgcct gctctgccac gtcctggaaa cgaaaactga aaaagttgtg acacagtctc
    10021 gcttcgatcc cctttgcgaa ggttgcgata gtaaagaaaa ttgccgcgaa aaagcatttc
    10081 tgggtacgcc tgtcatttta caggatcgtt gtgttggggt gataagtttg attgccgtta
    10141 cccacgagca acaagagcat atcagtgata atttacgcga attttctgat tatgttcgcc
    10201 atatatccac catttttgtt tcgaaacttc tggaggatca ggggccagga gataacatca
    10261 gtaaaatatt cgcgaccatg atcgataata tggatcaggg cgtattagtt gttgatgatg
    10321 aaagtcgggt tcagtttgtt aatcagactg ccttaaaaac acttggtgtt gtgcaaaata
    10381 atattattgg gaaacctatc cgtttcagac cattaacatt tgagagtaat tttacccatg
    10441 gacatatgca gcatattgtt tcgtgggacg ataaaagtga attaatcatt ggtcaattgc
    10501 ataacattca gggccgacaa ttatttttaa tggcgtttca ccaatcgcat accagttttt
    10561 ctgtagcaaa tgcacctgat gaaccgcata ttgaacaatt ggttggcgag tgccgtgtta
    10621 tgcggcaatt aaaacgactc attagccgta ttgcacccag cccatccagc gttatggtgg
    10681 ttggtgaaag cggcacgggt aaagaagttg tcgcccgtgc aatccataag ttgagcggaa
    10741 gacggaataa accctttatt gctatcaact gtgccgcgat tccggagcag cttctggaga
    10801 gcgaactgtt cggttatgtt aaaggcgcat ttactggcgc ttctgccaac ggtaaaacag
    10861 ggttgattca ggcggcgaat acgggcacgc tgtttctcga tgaaataggt gatatgccat
    10921 taatgttgca ggctaaatta ctgcgcgcta ttgaggcacg tgaaattctg ccgattggtg
    10981 ccagtagccc aatacaagtc gacattcgca tcatttctgc aactaaccag aatttggccc
    11041 agttcattgc cgaaggtaaa ttccgcgaag atctcttcta ccgacttaat gttatcccga
    11101 taactctgcc accgctgcgt gaacgtcagg aagatattga actactggtg cattactttt
    11161 tacatctgca tacccgtcgt ctgggatcgg tttatcctgg cattgctccc gatgtcgtcg
    11221 aaatattgcg taagcatcgt tggcccggaa acctgcgcga gttaagcaat ttgatggaat
    11281 atctggttaa cgttgttcct tcaggtgaag ttatcgacag cacgctattg ccgccaaatc
    11341 tgctgaataa tggcacaacg gagcaaagtg atgtaacaga ggttactgag gcacacctgt
    11401 cactcgatga tgcgggcggc acggcgctgg aggagatgga aaagcaaatg atccgcgagg
    11461 cgctttcacg tcataacagc aagaagcaag ttgctgatga actgggcatc ggcattgcta
    11521 cgctctatcg caagattaag aaatatgagt tgttaaacac ataacatcag tgaatccggc
    11581 ctgaattcag gccggatatc cttttacttc gttttctcgc aatccagaac tgtatttaca
    11641 atcatctgat accccgtgca gcgacaaaga tttcccgcca gtccgcgacg aatttccgta
    11701 atggttaatg gcttctcgcg tggtttcgcc agcattgccg tggtagccat aatcaggcca
    11761 ggcgtacaaa acccgcactg cactgcgccg gatttcgcat aagcctgctg aacatgagaa
    11821 agttttccgc ctttcgcttc accttccagc gtgcggatct cttttccttc agcccaggcg
    11881 gcaaggtata agcaactgtc tattgctgtg ccgtcgacca gcaccgtaca ggcaccacat
    11941 tcacccacgc agcacccttg tttgacactt agcagccctt gttcgcggag taattccgag
    12001 agcggcgtgc ctggtacggc atgaagctga aaaggcatcc cgttaatggt gcattcgatg
    12061 gtaattgttt cgctgtgatt cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa
    12121 tcactttttt ggtcatcgtc tggatgagat gcagacgaaa ctctttactg gcccgccatg
    12181 aagaacgcgg ggcgacatct tgcaggacag attcgctgat agcttccagc gtttgcaggt
    12241 ttaatggcgc attttgtgca gtctgttcgg catgttggca gcgaatcggc gttggcgcgg
    12301 caacaccaaa tgccaggcgt aattcgctga aattgccgtt atccagtcgg caatgtgcgg
    12361 cacagccaat cgttgaaata tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc
    12421 ccacgtgttc tttcggctgg ggcggaaaat gaaaggcgac aaggatttcg tcatgctcaa
    12481 gagacacttt gcccggcccg gtgtgaaagc cattaatcgg gacgaaacga acaccgcgtg
    12541 gggagtggat ctccagtttc gcgtcataaa tgagcgttgg cgtggcagaa tctgcgctgg
    12601 tggcaccgtt gcaaatattt ccaccgtagg tagcgacgtt acggatctgc ggtccagcaa
    12661 tggacgaggc cgcagcacat aacgccggga gatgacgttg agttatagaa tcttctatta
    12721 gctgggtaaa tgtcgttgca gagccgatac gtagcgagcc atcttccgcc agcgtaattc
    12781 cccgcagctc cgccagatta tgaatatcaa caatatggcg ataacggtca ttgtgatggt
    12841 ggagctgaat cagtacgtca gtgccaccgg cgagcagttt ggcctgcggg ttgtcagcca
    12901 gcaggttgat ggcatcggca agggttgctg cgcgatggta agaagcaaaa tcaaacatga
    12961 tgttatcctc aaatcaatcc tgccagatgg aactcttcat ataaccgttt tggcgtcagc
    13021 ggcagtgtat tgattgcaac accggtagcc atcttcaccg cgttacgaat agcagcggca
    13081 acaggaatta ttggtggctc acccagtgac ttatgtccgt atgcggattg cggctcattg
    13141 atttcgacga acgcgctttc cagttgtggc agatccggca tggtcggcat tttgtaatcc
    13201 agcagattgg ggttacggac cacgccgctt ttcgcatcga tgatcatctc ttcaaatagc
    13261 gcccagccaa tgcccattcc cattccgccg tgtacctgac cttctgccag tagcggatta
    13321 agaatatggc ccgaatcatg aacgttgagg atgcggttga tggtgacttt gcacagcgca
    13381 atatcgaccg tcagatcaac aaaggtacag ccgaacgccg gtgggttagt ggtggttttg
    13441 atggagcttt cagcagagag ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc
    13501 aaatctttta acgacattaa cggctcttcc ggtcgttcaa ccagcacgat atggcctttt
    13561 atcagggtca gattcatcgc tgactgatgt agcatgactg cggcgtgagc gatgattttc
    13621 tcttttaata acagtgccgc actgcgcagc gcaggcgcgg caacatagct ctggcgtgag
    13681 gcaaatgcgc cgggatcgaa cggcgtaacg tcggtatctt gtgttgaaat aacgcgaacg
    13741 tcgctgaccg gaacccccac ggtttctgcc accatttgcg agaagacggt gtcggcaccc
    13801 tgaccgattt ccgtcgcgcc gctttgcacg ttgatggttc catcctgatt catcagaagg
    13861 cgcgcgcctg ctatttctac gccgacaggc caggtgttag aggtgtagct aaaacaggcg
    13921 acgccaacgc cgcggcgtaa attgccttgc tggttctggc actctgcacg gcgtttttcc
    13981 cattcaaaga ttttccggcc tttttcaaga cactccggca accctgcgct gtaaatacgt
    14041 ttgcccgtga gcggattagc atctccttcg cgtgaggcgt tgcgtaaacg aatttcaaca
    14101 ggatcaatac ctaacgctgt cgcggcgtca tcaagcatag actcaacggc aaatacgact
    14161 tgtggcgcgc cataaccacg catcgcacca gccgagggga ggttggtata gcaggtcttt
    14221 gaactgtaag cgtaggcaca acgaggataa aggtaagcga ctttatttcc ccctgcagaa
    14281 gcgatggagt gcccgtgaga tacataagcg ccggtgttag acagaacatc cagactataa
    14341 cctttcaatg ttccgtcgcg gttcacgccc atttgcccgt caatagtaaa agcgtggcgg
    14401 gtacgggttg cgaggaaaca ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca
    14461 agcttgctgg tcaggaatgc cgccattggc tcttccagta catcctgttt attaccaaaa
    14521 ccgccaccga caaatgtttt gatgactcgt acgcatgacc agggaatatc cagcgcctga
    14581 ccaaccacgc ggcgaacaat gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag
    14641 tcatcctcca tccacgccag cgatgttacg ctttccatgt gacaatgttg aataacgggg
    14701 gtctgatagt gcccctgtac ctggtagtcg gcggcatcga ttgtttgttg gacattgccc
    14761 gtcgacatcg tgctttgttt cagtaaattg ccgccgttat ggattggtgc agcgtcctct
    14821 gccagcgccg cttctggcgt ggtgataacg ggtaattctt cccactcaat gctgaccaat
    14881 tgcgccgctt tttctgccgt gagttcatcg cgggccacga cgatggcaac ggcgtcacca
    14941 tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca
    15001 agtgtccatg catgcccagc tgtagcgaat gggatatcag gcacatcttc ccaggtaaaa
    15061 atcgccagta cgcctggtaa acttctggct tgttcatcat taatacttac ggcataacca
    15121 tgtgcgatag ggctacgtac atatttcgca taacacatgc ccgccataac ataatcgtca
    15181 gtatatcgtg cccgcccggt gaccttagca atggcatcaa cgcgcatgca tgattcaccc
    15241 gtagcggttg cttcccgcgc ttccatagaa atcccctcga tttgaacatt tggttcttat
    15301 ggcaaagatt tagtctaagc aggcaagatt tacgccagaa acggcgataa gcgttaaggg
    15361 aggaaggaag tgtgatgatg ttaaaaaaac acagatatga tacgaaacag gaccctgaat
    15421 aggggtagtg atacgtatca atatgagtgg cgataaaatc aatgtgataa attaggatta
    15481 ttacgcatca ggatgggaaa tagggtaaaa aagtcaggaa cttcgatacc tctacagtga
    15541 atattttgct gatgatactt ccctttgcca aatgaaatta acctctttct ctattacatc
    15601 gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata tggcgtcttg
    15661 ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc tctgggtatc
    15721 gtgatgttgt tatcggttgg actgcttttg gcgggctgtt cgggtagcaa atcatccgat
    15781 acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct atatcgtatt
    15841 tcgcgcacca cgggaaccag cgtaaaagag ctggcgcgac tgaacggcat ttccccccct
    15901 tacaccattg aagttggtca gaaactaaaa ctgggtgggg cgaaaagtag cagtagtaca
    15961 cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc atcagcggta
    16021 ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac gacagggaaa
    16081 gttatcatgc cgtattcgac agcagatggc ggcaataaag ggattgatat ctcagctcca
    16141 cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg caaccagctg
    16201 cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac ggcttacgcc
    16261 cataatgaca cgatgctggt aaataatggg caaagcgtga aggctgggca aaaaatcgcc
    16321 accatgggga gcacggatgc ggcatctgtt cgcctgcatt tccagattcg ttaccgtgca
    16381 acggcaattg atccgctacg ttacttgccg cctcagggca gcaagccaaa atgctgatgg
    16441 cgaattaatc agcagtcagc agcgcggcac ttgctaagaa gagcgtaagg tttataatgc
    16501 cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc aagctctata
    16561 cgagggttcg attcccttcg cccgctccaa tttatcttca cctcatcaat ctgccatttt
    16621 tgctgtatct gattttctat attatcttaa ttcaatcgct tcagactttt cgcaatctct
    16681 gtaacactcc cccatttttt tgtaaataaa aagcgtttac gcaatgcaat ccttttgtaa
    16741 caacccaggt aaaatacctt attttcaagt tgttaaataa gtggttatct acgcttttaa
    16801 caaacgattg atggaatatt ttatgaaagg gaaatctgca ttaactcttc tgctcgctgg
    16861 tatattttcg tgcggcacat gccaggcgac aggggcagaa gtgacgagtg aatctgtatt
    16921 taacattctt aattccacgg gggcagcaac agataaaagt tatttatcgt taaatccaga
    16981 taagtatcct aattatcgtc ttctgattca ctcagccaaa ttacaaaatg aaataaagag
    17041 ccactatacc aaagacgaaa ttcaaggatt gctaacatta acagaaaaca cacgaaagtt
    17101 aacgttgacg gagaaaccct ggggaacgtt tattcttgct tctactttcg aagatgacaa
    17161 aacagcggca gaaacccact atgatgctgt ctggctgagg gatagtttat ggggctatat
    17221 ggcattagta tcagaccagg gaaatagtgt agccgcaaaa aaggttctgc tcaccctgtg
    17281 ggattatatg tctacgcccg accagattaa gcggatgcag gacatcatta gcaacccaaa
    17341 acgactggat ggtattcccg ggcaaatgaa tgctgtgcat atttgacagc aactcccccg
    17401 tgatggcaga tgtgcaggaa gaaggcaagc cgcagctgtg gaactataaa caaaatgatg
    17461 cgttaggtct ttatcttgat ctccttatac aagcaatcaa tactggtact attaacgctg
    17521 aagattggca aaaaggtgac cgactaaaat cggttgcgct gctgattgct tatctggata
    17581 aagccaattt ctatgttatg gaagattccg gagcgtggga ggaagatgcc cgattaaaca
    17641 catcttccgt tgcactggtg acctctggac tggaacgttt gtcaaaccta ctctcgaaga
    17701 aagactctgt ttttgtctct gatttactac gcgaagctaa agtcaatgaa ctggatgaaa
    17761 ctctttcaac aacgaggttg aaccacctca tagataaagg ttatgaaaga ataacgttac
    17821 agcttgattt agggggagaa tccccaggtt atctggaaaa agataaacat tacagagaag
    17881 ccgatgcggc tttacttaat gttatttatc ctgcaaatct ttcaaagata aacaccagaa
    17941 gaaaagagca agttctaaaa atagttaaaa aactagcagg tccgtatggt ataaaaagat
    18001 acgagaagga taattatcag tcggcaaatt tttggttcaa tgatattaaa accgataccg
    18061 atcaaaattc gcatgcaaaa agagaaaaga gcttcattcc atcaacagaa gcagaatggt
    18121 tttttgactc atggtatgca aaatcagcag caattgttta taaagaatca agaaaagaag
//
LOCUS       DAZFTN010000091        17980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFTN010000091 DAZFTN010000000
VERSION     DAZFTN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(240..392)
                     /locus_tag="ACHKV2_003548"
                     /pseudo
     CDS             complement(240..392)
                     /locus_tag="ACHKV2_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(389..509)
                     /locus_tag="ACHKV2_003549"
                     /pseudo
     CDS             complement(389..509)
                     /locus_tag="ACHKV2_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(549..812)
                     /locus_tag="ACHKV2_003550"
                     /pseudo
     CDS             complement(549..812)
                     /locus_tag="ACHKV2_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            906..1255
                     /locus_tag="ACHKV2_003551"
                     /pseudo
     CDS             906..1255
                     /locus_tag="ACHKV2_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1913..2344)
                     /locus_tag="ACHKV2_003552"
     CDS             complement(1913..2344)
                     /locus_tag="ACHKV2_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO6941369.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2412..4433)
                     /locus_tag="ACHKV2_003553"
                     /pseudo
     CDS             complement(2412..4433)
                     /locus_tag="ACHKV2_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4567..4692)
                     /locus_tag="ACHKV2_003554"
     CDS             complement(4567..4692)
                     /locus_tag="ACHKV2_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO6941370.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(4634..4783)
                     /locus_tag="ACHKV2_003555"
                     /pseudo
     CDS             complement(4634..4783)
                     /locus_tag="ACHKV2_003555"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(4957..5718)
                     /locus_tag="ACHKV2_003556"
     CDS             complement(4957..5718)
                     /locus_tag="ACHKV2_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6941371.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(5715..6149)
                     /gene="psiB"
                     /locus_tag="ACHKV2_003557"
     CDS             complement(5715..6149)
                     /gene="psiB"
                     /locus_tag="ACHKV2_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6941372.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(6204..8162)
                     /locus_tag="ACHKV2_003558"
     CDS             complement(6204..8162)
                     /locus_tag="ACHKV2_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6941373.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(8228..8461)
                     /locus_tag="ACHKV2_003559"
     CDS             complement(8228..8461)
                     /locus_tag="ACHKV2_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6941374.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(8518..8970)
                     /locus_tag="ACHKV2_003560"
     CDS             complement(8518..8970)
                     /locus_tag="ACHKV2_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6941375.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(8996..9202)
                     /locus_tag="ACHKV2_003561"
     CDS             complement(8996..9202)
                     /locus_tag="ACHKV2_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6941376.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            9272..9703
                     /locus_tag="ACHKV2_003562"
     CDS             9272..9703
                     /locus_tag="ACHKV2_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941377.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(9789..10352)
                     /locus_tag="ACHKV2_003563"
     CDS             complement(9789..10352)
                     /locus_tag="ACHKV2_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6941378.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(10399..11760)
                     /locus_tag="ACHKV2_003564"
     CDS             complement(10399..11760)
                     /locus_tag="ACHKV2_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO6941379.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASLAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(11812..12042)
                     /locus_tag="ACHKV2_003565"
     CDS             complement(11812..12042)
                     /locus_tag="ACHKV2_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941380.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            12559..13068
                     /locus_tag="ACHKV2_003566"
     CDS             12559..13068
                     /locus_tag="ACHKV2_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941381.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(13038..13229)
                     /locus_tag="ACHKV2_003567"
     CDS             complement(13038..13229)
                     /locus_tag="ACHKV2_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941382.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(13226..13648)
                     /locus_tag="ACHKV2_003568"
     CDS             complement(13226..13648)
                     /locus_tag="ACHKV2_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6941383.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(13695..14120)
                     /locus_tag="ACHKV2_003569"
     CDS             complement(13695..14120)
                     /locus_tag="ACHKV2_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6941384.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(14538..15314)
                     /locus_tag="ACHKV2_003570"
     CDS             complement(14538..15314)
                     /locus_tag="ACHKV2_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941385.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(15360..15914)
                     /locus_tag="ACHKV2_003571"
     CDS             complement(15360..15914)
                     /locus_tag="ACHKV2_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6941386.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(15808..16029)
                     /locus_tag="ACHKV2_003572"
     CDS             complement(15808..16029)
                     /locus_tag="ACHKV2_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941387.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(16030..16713)
                     /locus_tag="ACHKV2_003573"
     CDS             complement(16030..16713)
                     /locus_tag="ACHKV2_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6941388.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(16790..17095)
                     /locus_tag="ACHKV2_003574"
     CDS             complement(16790..17095)
                     /locus_tag="ACHKV2_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941389.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(17099..>17980)
                     /locus_tag="ACHKV2_003575"
     CDS             complement(17099..>17980)
                     /locus_tag="ACHKV2_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6941390.1"
                     /translation="AQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLLQTTEDVR
                     FEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSGTGRRRWE
                     ELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDLLTVLSTR
                     LDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDFDTPWCQP
                     ESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSPTDDDELP
                     EVTAPEWIVDKVAHYGG"
BASE COUNT         3899 a         5356 c         4604 g         4121 t
ORIGIN      
        1 agatatcatc atttttttta tagataaagt tcctttttct ctcttgaatt tggccatgtt
       61 taacatcagt actaacattt ttaactcttg tattgttaat tgctttatct aaagaagagc
      121 caggatgaat catatctata ccctctaatt atgtaacact taataatttt tgattagcga
      181 caacctaaaa tgtattttga tgttgttata taaattaatg tattcggtgg tgcatccaat
      241 tagtagaaca tgtgtttttc gataaacgct ccgatcactt tttcgtggat ctcaactgag
      301 cgtgagaagc agatggtttt acgagtcaac cgcttaatgc gggtgcgtag cgtcagatta
      361 ttacgctcaa tgcgttgggt gaatattttg ccagcacgtt atcaccgaac atctctcccc
      421 cagtcggcga accctttatt ggacgtcagc acaatgctcg ctttttcata tcgacggttc
      481 agcaaccgga agaacagact gtgttcctgc gtaaagcgct gctccggtgt cttcctgaac
      541 tggcgaagca acatggaacg gccaccggta taacccatcg ctttgatttc cgccaggata
      601 acttcgccgt tccagacatt ctcagccaga cgcatgtcga tatagtccat ttcggccatt
      661 ttgtggcgtg tttttctggc cggaggttct gggtatttca ggtaacgtct gaccgttcgt
      721 tcagagcaac caatctgagt cgcaatatca acaatatacg caccttgctg gcgcatttgc
      781 tttatcatgt aaaagtcctc tctgctcagc atgctgatgt cctttctggt gtgagaacct
      841 caaggaaaca acatgttggg tggagcggac aatccaaatg gtgaattacc gtcttatatc
      901 actggcggat aaggataagt acacgtcggc agttccgtca gcactgcgac agcgttgtcc
      961 tcgttgcttt tacccggtca tgcacaacgc ctgatggatg aactgcgtgc ccagggttac
     1021 cattttaaag taaaaaccgt gacggaaagc ctgcgccgtc acggactgag ggcaaaagct
     1081 tcctggaatt tcagtccggt ctgttaccgc gcacacagcc agcctgtgtc agaaaatctg
     1141 ctggagcagg atttttacgc cagtggcccg aaccagaagt gggcgggaga catcacatat
     1201 ttacgtacgg atgaaggctg gccgtatctg gctgtggtca cgtgccatta ttggctgatc
     1261 aatgtcgtca cgcatgacgg cacaggagcc cccggaacgt tatgctaccg catgtcattt
     1321 gcaaaaatga cagatagata tacttcaatt tgaatcttaa caattagcat gggaatgttg
     1381 tcaatcctct gtatggagcc accacatatt gcaatttttg ttctctttta aaatatattc
     1441 cacgagcgtc ttgtgggacc ttttgctgtt ataggggggc agtttggata tcgaaaatta
     1501 ttgtacgtta aggctgaatt tcaatcgcaa agcataacct tacctttctg cttacttatg
     1561 cagttcaaag agtaccaact ctgcaatttt cgttgccaca gatgcaatat cgttactttc
     1621 cagtcaccat gccgctgaaa gcccacacca tgcgataatc cacatcaaga gccgctttct
     1681 gtccagaccc gctgttgtga ggtgtgaggt gtgaggtgtg aggtgtgagg tgtgaggtgt
     1741 gaggtgtgag gtagcctgag tttaacggac aggccgatga aggacgccgt taccgggata
     1801 atgtaagaat ctttttttga gtatgtcaac ataacctgag gcaaagtacc tcaggttcca
     1861 gtaatcagcc gacctgacag tcttctgtta gccctacaga tatgtcgtgt agctaaccgc
     1921 gcatgtcaaa gccggatttt tgaaaatctc tataaacttc cggattgttg aaggccggga
     1981 atttagcttt atgggctgct gactttatcg cttcgcaata ggctttatcg ccattactgg
     2041 ttgatatttt taacgccgtg ccatcccggg agaattccat atgcaacctg cattttttcc
     2101 ttttccattt ttgcggatca tcaagtttag cattaattgc atccctgatt ccccgtgcct
     2161 gctcccccca ttcatcctga tcatcccaac gtcctgaact gcaactaccc agggctgtag
     2221 ttttatggca atttgaagga tgtaatggcg cacatcccgc cacaaagctg actaaaatag
     2281 ccagtataac gattttcttt acccccatat attgctcctc aacccatata gatctcagta
     2341 ccatagctct gtcagagcaa agaaaatctt aactaataca agttaagatt tttaatatcc
     2401 actcctcatc cctacattcc aggtgatggt ggcgtccact cattatcctg aactggagca
     2461 taagatgcgc catgcccccc ggatatgctt ttctgttgcc tgatatattc gtggtagtac
     2521 tgttcactac gctgacgatg taaatccaca tccgggcggc tgattacaaa gtgtccattt
     2581 ccggttattc cagctgcatt atgccaggcc accatttccg caaatacatc attaaactgt
     2641 tgatatgagc cttccggttc aaaacccatt cctggctcac gatcaaacaa tacaggagca
     2701 atcagattca gcttatccac cagttcattt ctgtcctgat gatggtagaa caccacacgg
     2761 tccccataat ccacacagac taactcgcca ttcctccgat cacgaaaacg cacactgcca
     2821 tctttctgca gacgggcttc gagaacgcca gtgtcttcat aaagtggagt tcctcccggt
     2881 tcacagagaa tcacgcagcg gtcggcattc gttgttcgtc gcttgtcttt gcgacgatca
     2941 cgataatccc agccacgtaa ctgtgataat gcagctctgt ctccctgtac cgcctgttgc
     3001 tccacccact gccggtagga caaaggatac cacttaccct cctcaatcag tgacaaccgc
     3061 tcttccttca cactctcctt cagcctgatc agcgcctgca tacgctgcac ttccgcaata
     3121 tggtaatgca gcttacgcaa ttgtggatcc cggaactgca cacggatata cgccttgcgc
     3181 cgacggcagg cagcatgaat ttcccgtaac cgttctccgt aacgtaagtc gggtttacgc
     3241 cagtgctcct tccatgcaag ataacgcgcg cgcaaatctt cccgcgcagc tgcacgggct
     3301 tcacggcgtt cacgacgcag tacaggatcg cgccggaagc cgggctccac ttcatcgctg
     3361 gcagcaagtt ccggattgta gcgacattca ggcttcaccc gttcaaatat atctgcacct
     3421 gcaatctcaa atggccctgc ctgcggctca gccctggata acgtcaaatc cgggtggata
     3481 ctgctggcct tcactggtgt gagctcatga ttaaacgcat ccacaatcac aagaccatga
     3541 tgctgtttct gcaacataag ccctttacgc gcaaacatct catggcacgc ctgccagctg
     3601 aaattcccgc ccgcgatcat atcttcaatc aacgactcac actgaacagt tgtgtacccc
     3661 cacagacttt ccttatcaga aaacagttcg cgctttattg ctccctgtgg acctgtcccc
     3721 atccgcatac gataccattc gtaatcactg acagcctgag taccaagatt acgtccccgg
     3781 aaccgttctc cgggtataac ctgagtaaaa atatccttcg gtacaggctg ccatccacca
     3841 tccagatcct gcatataatc cagcgtcatt aacggccaca cactgtctgc acgtactggt
     3901 gtctgttgtt tatcaaatcc atcatgcagt accagatgcc cgtgctggat ccccatccat
     3961 aatccagccg ttgccagagt tctgtgcaca tccaggcaac tttccagctc accatttctc
     4021 gccatttccg gtaagcgttc ggcaaaatga cggcaggcgt actgtttcag actctccgtt
     4081 tctgccactt tttccggccg gatatcaacg tttatggctt ccggatcata acgaccgggt
     4141 gtccccacct gactgaaaat atctgtcggt acaggctgat attcccccag tttccgcccc
     4201 agtttttcag ctgtccataa cggtccgaac gaactgagtg caacaccttc ccgcgctctg
     4261 tcccagccat ccttcaccac cagttcccct tcctgcatcg tgatatacag accatcactg
     4321 gccagccgtt tatgcaaaga taaccagtcc tgtgcgggct cttcacgcag cccggcaatc
     4381 gacatctgag caatatattc cctgaacgtc tgctttttcc cgcgtcgcca ggctgttcat
     4441 gcccgtacaa tgccagcaag gagaaagggg ccaccggcga accagcagcc cctttataaa
     4501 ggcgcttcag tgcagtcaga ccagcatcag ccctgaaaag gcgggcctgc gcccgcctcc
     4561 aggttgctac ttaccggatt cgtaagccat gaaagccgcc acctccctgt atccgtctct
     4621 gtaacgaatc tcgcacagcg attttcgtgt cagataagtg aatatcaaca gtgtgagaca
     4681 cactatcaac acacaccaga caagagagct gcgtggtagt ttcatggcct tcttctcctt
     4741 gcgcaaagcg cggtaagagg ctatcctgat gttgtctaag catgcagggg cctcgtgggt
     4801 taatgaaaaa ttaactacgg ggcttttgtc cttctgccac acaacacggt aacaaaccac
     4861 cttcacgtca tgaggcagaa agcctcaagc gccgggcaca tcatagccca tataccagca
     4921 cgctgaccac attcactttt cctgaaaata acccgctcat tcaggctgtt tacgggcaat
     4981 ccgtgtgatt gttgccgcat cacgcacctc ccggagtttg ttcggcacca tccaccgctc
     5041 catctcacgg acagcatgac cggcctcctt tgctgccaga ctggcttccg cgataacctg
     5101 ccagaatggc atatcctgcc actgccagcg ttccattccg gccagtgacg gaaaacgctc
     5161 gccccactgc cagaacaggg tttccaggtc atgctctgcc acccgatcac tccagcgtgc
     5221 ataccagctt ccgactgttt ccggtgaatg gaacgccagt tcgatttcag cctgggcgac
     5281 cagcgcctgg tacgcacggg ctttctgctc tgcctccctc gcctgctggc ggctgtactt
     5341 ctgcatgacc agcgcggatt tatggcgact gcgttccctg acacaaaacc gcaccgacgg
     5401 gaacaagcgt tctgctgcat caccgggaag gggaaggagg cagacctcgc cctgtgtttc
     5461 aatcagcaca tcgatggcat acaaccactg aagacggttt ccgtgcagtt cttctgcggt
     5521 cagggacggc atgaaaaatc gcaggtcaga cagcgatatc ctgcggcttc cgttcagaca
     5581 gcggaaaaac gcccccgcat acggatattc cgccagcgtg tttccctgat tgcggcgttt
     5641 ttccgtctct gccactgccc gccatgcggc ctgttgctct gtgctgaggg gaaccagtgc
     5701 ctgtgaacgt gcgctcatac ctgacctcct tccgccatca ggatatggat gatgtcgtta
     5761 acgctgtatc cctgcgtatc cagtgacgcc gccagtgcca gtgaatggct gacggcttcc
     5821 ggtgcaaacc gccgctgtac ctgaaccacc gcaaacggct caccaccggc attcaccaga
     5881 accagcaccc agacctggga gacatcgccc ggtgaacata acgccaggtg aaaacgttcg
     5941 tgggcaggcg tgaaacggac ctggacgggg aaaaagccgc caaactcgct gccgtactcg
     6001 ccgttcatcg tccagttgtc cggcgcatcc agctcccgca tcacggcgtg tgtcagctca
     6061 cggcgtgcat cacttccggc agcccggata tcttcatatt cctgtgcgtt catggtatgt
     6121 aatacattca gagtcagttc agttttcatc acggtatctc ccttgctgcc gccccgtctc
     6181 cggcggggcg gtgtggttgt tcatcaggcg gcatcagcca ggttatcggt gcgttcagtg
     6241 gcatccgtct gtgggcgtgg tgcacacatc cagccaggca cccagcggtt gtctttcata
     6301 tggtgctctg catgttcagc cgcatcgcct ttcttcatct tctccgcgtc ccgtgcggca
     6361 ccggatagtc ctgcctcatt cagggctgcg ataatctgcg gctttttcag gtgtccgaaa
     6421 aagtttgctt tcgtcggctg ccaccagtcg cgcatgtgaa agccgatggc gctctccagc
     6481 gtgtccagcg gactgcgtga cgtgtgacca tactcacggg tctggacacc gtggatactg
     6541 cacgcggtgc agaaactgag cagtgataac agcacctcct gactgagtga caggaacgtc
     6601 gtcatatccc gtgcccatcc atccggcagc agggcggcaa gacgggcttt ttctgccatc
     6661 atcgccatga atgcggcacc ttccttccct gacggcgcat cgctggtcag cgaataatgc
     6721 tcacattcca ggcggattct ggcgggattg ctgtacgctc cactgccaaa tacattcaga
     6781 cagagtgtcc atgccagaag tgccagggat ttgtctgatt gctgcatcag tgccgcctgg
     6841 actgccagcg tgcgctctga ggacattttc gtcagcagcg gcagactgat ttcctccact
     6901 gaggctttct cctgcacctg ttccgtgcgg taatcgttat cagccacatc atcctcactg
     6961 cgcaactgca caccacgctg gacacacacg ttgccataac gccaggacac caccacgcca
     7021 cttccggcac gcatctccgg cgtccacgcc ctgaccttcg ccatgcagcg catcagcttc
     7081 atttcggctt ccagcaggtc ggattcctca cactggtttt ccagcgcatc gtaacgtgcc
     7141 atcagttcgt tcaggcgttc ttcttctgcc tccgtcagca ccgcttccgg ctccggcaga
     7201 ctgcggtatg ttccggcatc ctcacggcag aaaccaacag gctccatacg tccggcgcac
     7261 cattcccagc cttcggcttc ccgaaggtgt tcggcgacag cctggagttt ttccagcagg
     7321 gcggcatcga gcgccacgca gtccacatag ccaccctcgt cgtcactgaa caaatcggtg
     7381 cgcagttcat ctggcgagaa ggtatcagcc cccacgaagc ggaatttact gttccctgcc
     7441 accgccactt cactttcggt caccagacga cgaatggtct gtacttccgg tttaccgccc
     7501 catcccgact gacaggcggc ttcaaacacc tgcacctgac gcgcggtgtc gttctccagc
     7561 gccagcgcct gacagtgctc ggtggtgatg cggtcttctg ccagcgcatc gaggatgaca
     7621 ggcgcaaggt ctgccagttt cagcattcgc tgaacgtggc ggggcgaata acccagcaaa
     7681 tcaccgattt gtgcaggtgt tttgccttcc tgcgccattg cacggaatcc ggcaatctgt
     7741 tcggcagggt gcatatcccg acgatgaccg ttctcggtca tcgatgcggc agtcgccagc
     7801 tcctgcggaa tgaccttcac acggacaggc cagtcagccg gaatgatgtc acgctctgcc
     7861 agcatgttga gtgctgccag tcggcgacca cctgcggcga caccgtaacg gtcacctggc
     7921 agggcatgaa caaccagatt ctgtaacagg ccgacacctt taatggagtc cgccagttcg
     7981 ctgacggact ccgcagaata cggcaccgtg cgcacattca gcggtgactt aatcagcgag
     8041 gccagcggca cgctcacttc ctccgtctgc gccagcaggg ccgacaggac tgtttcctgc
     8101 gtttttgcag gtttacggct ggatttacgc tccgtttttg ccttagactc tgtaactgac
     8161 atagttagta cctcccaaaa cggtaataat gattgtctgc cggacgcgtt cccgtccggc
     8221 acctctctta cttaataatc ccgaaatcac ggatgacatg gttcatccag tacccggcac
     8281 cgtcctcaaa attccaggta cgccagacca ttgcaccgtt attacggaca accaggcgaa
     8341 actgttcgcc gtggtcattc tcgataaaga cgttccggta ctgtgcggca acggcttcgg
     8401 catgtttgcg ggtaaaagga ccgtcaggca gtccctgaag tattctgaaa tattcgctca
     8461 tcctcatctc tccggtgatg ccccgcacag gacggggcgg ttgtcacagt cagtcgttca
     8521 gaacgggata tcgtctgaaa acccgtaatc gtcaccctcc ggcggttgcg gctgaggctg
     8581 accgctgaac tgttgcccct cttccggctg agtctgagca cctgcggcac gtaccagcat
     8641 ctgcatggtg cccgtggtct taacaagaat ttcggtgacg taacgggtga taccgttatc
     8701 ctcccagcta cgggtgcgaa gctgaccttc gatgtagacc tgcgcgccct tgcgcagata
     8761 ttcacctgcc acttccgcga gcttgccgaa cagcaccacg cgatgccatt ctgtctgctc
     8821 ccgcatctcc cccgtctgtt tgtcacgcca gctttctgac gtggccacct gcaggtttgc
     8881 cactgcgccc ccgttgggga tgtaacggac ttccggatct tttcccaggc gtcctacgag
     8941 gatgaccttg ttaatgccac gaactgccat gatgtgtctc cttctgttga tgttttcagc
     9001 ccccatcatc ttttgagttt cgggagccgc ggttttgacg ttttgcgggg acctgccttt
     9061 tgttccctcc ggtattcacc atcacctttt tgagaacctt cgccgcaagg ggcatcactg
     9121 ccacggccgg aaacgactgt ccgggggcag accgcaaaac tttttgcctg cttttgggca
     9181 aaaagttttg cgggatgtgc atttcacccc tggactggcg gagacggttg tggctgtctt
     9241 tgcccccggc gacaggggct taaacaggtg aatggcaccg gacacaacaa cgggcaggca
     9301 cccgccgcaa cgacgtgaag cgcggtttgc gaaacggcgt tgccccttac atcccggcgg
     9361 ccttttgccg ggatgccggg agcgcaggcc acggcgcgac agaggcggcg cgaccggaac
     9421 ggtcgctgca agcccgggtt tcgcgtcagg gatggaagcc cgccagggcg gagacagctc
     9481 cgctggctcc gttcacgaca gcccgccccg ccagggggac gcccggaaaa ggcataagca
     9541 ggagaacaac aaaacagaca gtaatcagag cagcagaaaa aagaagaaca gtagcagtag
     9601 cagtagcatg aacaacaaca tcatcagtaa cagacaaatg acaggtctga aaagacagaa
     9661 gcccacagca gaatgtccgc gtaatgtaag gggcggcgcg tgagcgcggc ccctttcagc
     9721 gccaccggcg cgcggtcttt tcccggcgcg ggcagacggg catttatcct cccgcctgcc
     9781 cggagtcatc aggcacgcag acgaataatc atcgtcggaa catcggtata agcaaacgtg
     9841 ccgcgtggca gctcctcgcg gacgtcagaa aacggtaaca gcttctcctg ctggcgcggc
     9901 ccgttcagac agacggcgac cagcacgcca cccggacgca acagggaaaa ggcccgcagg
     9961 atatgccgga tatcctgccc gtggctgaac ggcggattaa tgataacccg gctgtaatac
    10021 tgcaccggct gccattccat gaagtcgcca cactgaaccc tgacaccgtt aaaattttcc
    10081 cgcagatacc ggaccagccc gctgttgatt tccaccgcat cacacatggc ctccggtgcg
    10141 gtatcccgaa tcgcccgcag tatggccccg gtgccggcag agggttccag aatgtgatca
    10201 cggttgctga tctccgccag cgtcaccagg cgatcacaga ccggggcagg agtcacaaaa
    10261 agctgagatt cagcccggat ttcagcaacc ggcgcggcgt tatccagcct tgcgcgcatc
    10321 agccggatac gttcttttaa ctgactgtgc atgtcattat ctccgtcatc ccccggggat
    10381 ttcccggggg gatatacctt atttctgtgg gatttccgta attttcatgt cggtgatata
    10441 cacattcacc agacggaaat tattgtccat tgtgcggcgg tagcggtatg ccccgtggtc
    10501 ttctgcttct gccacactgc gcacggcctt acagtcccgg ggcagggcgg cccactgtgc
    10561 ctttgtcatt tcccggaaac cttcccccgg atagttgact accggcggac gcttcgcggc
    10621 caggctggcg acggcagcct cttctgccga tggtgctttg tagtccgtga tgcgatcggg
    10681 cgtcactttc atcgtgccgc tgtacccgag aaaactgtaa ttcggcgttg tgactgaact
    10741 caccgccccg ttgcttttgt tcacgcggat gatggtcagc cactcgcccc ggctgaaaac
    10801 ctgtccgccc ggctcaaaat cctgtgtccg ggtaaccacg ccgccgcttt cgtccagcat
    10861 ggcacgctca tagttcaggc ggttctgata gtggttaacc cagcgttgct gatgctgaat
    10921 ctgccgttca tgacagcgga tcgcaatttc gcgggcctgc tccgtggtga tgatgtcatc
    10981 atccagggcc gaccataacg accggctccc ttcatactgg cttttttctg ccgggcgcgg
    11041 atacgtatcc agcgggaaac aggcgctgat atggtcatga ctgctgatga gttttgccat
    11101 attcagatcc agcgattcag cccgccacat cgtcagatat ttctgcgact gcgcgatggt
    11161 cttttcagcc ttgcgcaaat cagcttcgat ttttttgata cgacgccagc gaacgtccgg
    11221 acgttcttta tacttcgcgt gaagcagtgc tgaccgcccc cgttcttccc agtattccgc
    11281 acgttcaaag agcatcacgg cacgtttcat gccgttttca atacgctgcg catcacgacg
    11341 ggcgcggcgt tcgctgtgat gccccacaag aatgggctga ccgggcggga tcatcgcggc
    11401 cagtctttcc acttcatcaa gtgcctgtgc ggattcactg gcccgctttc cgctgtatcc
    11461 ggtaaaccgc tccgcccgtg cttcctgacg ttcagcgagc gtgctgtctt catcctcaat
    11521 ctctccggca agtgagagga gcacgtcttc ccggcccggt gtccaggccg gcgcgacaaa
    11581 cagcgcctgt tttggtgccc agcgaaaacc ggcatcatgc acttttttat acgtctccgg
    11641 gtcaagacgt gacacggcat acaggcgcag tttgttgtca tccggtgagt aggtcgcgcg
    11701 gtacatctgc acagggctgg cggcttccgg ggcggtcatg gtgattgctg ctgatgtcat
    11761 aaaatgttct ccttccgtct gttgccgccc ctcgcggggc ggctcacctc atcagttaaa
    11821 agcgcgtttc agttcgagtg tgcggagcac ctgcccgaaa gccgtgtttt tcatggcggg
    11881 gatgtgtaca ccacgccccc ggtgaaaact ccgggctatg tgtcgcccga aggctttcag
    11941 cacggggtca atttccggag cggggatgtc ttcgcgtctg aaacggggat gacggcgggg
    12001 ctttacagcc gctgcggaag gtgaaacaat gcttacagtc ataaatggat tccttatgac
    12061 aaattggtta acaatgccgt caaaattggt cgcacaattc ttacggcgtg gattaagcag
    12121 gtccctgacc tgcttttttc atatctgcat ctgacgcttt ccggtgtttt cctccttctg
    12181 ttcctgcgtt ctcattacct gtcagccgga tgactgacag aacacataaa tgcgtattgc
    12241 cggagcctga aataaatcct gatttttaaa gaacatcacc cgttgagttt cgggagccgc
    12301 ggttttgacg tttcgcggga acctgctgct gtattccctg tggtatgaac ttcacccgtt
    12361 tgagaacctt cgccgcaagg ggcatcactg ccacggcctg acacacctgt cctgggcaga
    12421 acgcaaaatt ttttaccggg ttctgggtaa aaaattttgt gggctgttca tttaccctgg
    12481 acagtcagtg cggcaggttg tggctgtatc agctcccggc gacagggact catacgggtg
    12541 tgtggcaccc tgcaggcaat gagcagggac ccgccgcaac gacgtgaagc gcggtttgcg
    12601 aaacggcgtt gccccttaca tcccggcggc cttttgccgg gatgccggga gcgcaggcca
    12661 cggcgcgaca gaggctgcgc gaccggaacg gtcgctgcaa gccagggttt cgcgtcaggg
    12721 atggaagccc gccagggcgg agacagcttc gctggctccg ttcacgacag cccgccccgc
    12781 cagggggacg cccggaaaag ccataagcag gagaggaacg gaaagaagag gaggaagagg
    12841 aagaggaaaa agaagaagaa cagtaacagg cacggtaacc acagaaaaat aacatcagaa
    12901 aaaaacattc cggacacatc acaaacagga agggtaacgg acgacgcgtc agcgtggccc
    12961 gcttcccttc cggcaggcgt cagcctgcca ggggttttcg cgcgggctga caggcggcct
    13021 ccaccgccag ccctgtatca cctgtttctg ctgccctgta agatgtagtg accgcccagg
    13081 aaaacagcct gctgattcgc cacaaaatcg cagatttcat caacaatctg cgcggtggtg
    13141 agcggtttct gccccatttc acgacgtcgc gcattaatca cggctttgta attgcgcaga
    13201 atttcgcggg tttctgtgct gatattcatg acatgtgctc cccgtccagc gcctgtaaca
    13261 caacgtccat tgcttcgcgt tcctccctga cgaacgcatc accgtcgccc ccgttctcac
    13321 tcccgacggc atacaggcgc ttcatttcac tgcctgccag cgccatcact ttttccagca
    13381 tcaccgccgg aactgtcacc tgacggctgg cacgcttttc ttccatcaac tcacgaacaa
    13441 cgtcgtgaac aatgctgaca tccgcaccat attcaaaggc atcgtccagc cgctgcatga
    13501 cggtttccag ctcatcgtct gacaggttcc agtcctgggc tatgtggcgc acatccccac
    13561 gggtcagcac cacggcaaaa acgcattcac tgtccggcac gtctgtatcc agtacatcgc
    13621 ggatgatttc tttaacagta cagtacatgt tctcgtctcc tttacagggg cggcattgcc
    13681 gccccgtccg ttaatcagtc cagtgccgcg taaatcgcgt tgcattcagg gtgaaattcg
    13741 atgtgacgcc acaactgcgc atcgcgcatc cggtaaagct gggtcagtcc tgcatcaccg
    13801 ctgtcgtggt acagccacaa ctggcggtta atcacaaggg aagtcagaat gatgccgcag
    13861 gcatcagcac tcacggtacg gtcaaaccag tttgcaccgt tcaccatgtg gaaacggtcg
    13921 ccatccggca tcatgtaacc gccaccgtcc ggcaggcgga tgaagtgcca gaagcctccc
    13981 tgatattccg gcatcatttt ttgcgccagc gcgtacacat acgcctcggc atacagaaaa
    14041 tcgttgccaa acaggtaagg cagaaaggag agacggtcgc actcttcaac attcagagtg
    14101 acaggtttcg catattgcat gatgttttcc tccgttgttc acatattccg cgacaccggg
    14161 cggtgccgtg cctccagaaa agccgggcga taaggcgggg atgcacaggg cgcaagggaa
    14221 gccgcagcct gacgcacacg gctgacggga gttgcagggt gaggccgcga aacctgagcg
    14281 ggcgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc gcagcggcga
    14341 agcgtaccct tgcatcaacg ccagcccggc taagatggag gcacggcacc gcccgcacac
    14401 gacgctgagg ccacataact gaaaaaccac aaggccatgc cctgcatggc cgggtaaagc
    14461 ggagccagcg gcgacttcct ttgtgctctg tccggggacc ggcaggaaaa aggcggggat
    14521 ttaccccgcc aacgtcatca tttccggatt tttgccaggg catccgccat gctgcggatt
    14581 tcgttccggg cttcccggac cacctcccgc agaacaccac ggtcaaacca ccggcggggc
    14641 ggttcatccg gtatacgcag cacatcgcag gccagcccac tggacaggat atgttcaata
    14701 caatcgctga ccagatacat ttccaccgcc gaaaaacgtg cgccccggtt caggatggca
    14761 tcgagcacca cgggcaggtt ttcgtcgtcc tcatcgagcc agcaggggtg atcgctttcc
    14821 ttcacgacca ggcgaaagcg gcgcgtctgg cgggtcagta acgtctgacc ggtggtggtc
    14881 agcgccgccc ggatacgccg cgtatcttct gccacccggc aactgacact gcgtaccgca
    14941 tcgcgcaccg ccgcaagtac agccctgatt tcgtcttcgg tcagccattc accggcatac
    15001 ggaaaagaca cctgcgttcc gtctgccgga agcaccggca gcgactgcca ctgactgccg
    15061 gacagaacaa gttccgctcc ggttccggtc agtttctgaa tggcggaggc caggtgcggc
    15121 ggcagtacgc cgccgtggcg gttaacgagt aacgtcgcca gccggggcgc atccagcaga
    15181 acataaatgc tgatccagcc cggatacttc gggttacgct ggtcgacatc cgtctcttcc
    15241 ggccagccct gcgccagcag caggttacgg gcgtgacgac aaatcagtgc gttcagcgtt
    15301 gtgttttcgt tcatgattaa tcgttctccc tgtatcgccg cccgcaggcg gcgataacat
    15361 cagccgtttt cgtgtgtgtt gtttttcagc aggatgcggg cctgattaac caccgcctga
    15421 cggcgggtaa cgcattccgg gacggcgaga ccgtaatcac gggcggccca ttcacgtttc
    15481 cacagtgcct gttcggcggt ctgaatcagg gacgccagca gttcagccgg gacggtcacc
    15541 tggcggtttt cgctcacgtt gctgataatc tccatcgccg cggcagaaga aataccggac
    15601 tcaattcgct ggtcttccgg gatgtcctcc aggcgcgcca ggacggtcct gatttcagga
    15661 tcggacaggg aaatatccat tccgtcagcg agggcctgaa tctcttccgg ggaccagata
    15721 accagcatca gcggtgtata tcccggatac tttgctgcca gctcccggca tagcgtttcg
    15781 catgttccgt acattctcgt ctccttctca atctttaaag cacgcatcca gttccagaat
    15841 ttcggtatac gcctccatga acaggcgatt ggcgcgatac agcgtctggc gtgccaggcg
    15901 ttcaacaagc acatcggaat caaagcgaca gcgatgctcc atatcgttca gcctggcccg
    15961 aaggtcacac atggtgttgg ccacctccgc cacgtcggcg cgaagttttt catgtccggc
    16021 atagttcatt tacgcagcct ccggcataaa ccagtcatca tttgcggccc cctgctgcat
    16081 ggcccgctgt acggcagcca gacgttgctg tccggcacgg tgatactgtt caagcagctc
    16141 gataccgata taccggcgtc cggactgaag ggcggccaca caggttgagc cgctgcctgc
    16201 aaacgggtcc agcacaatcg cgttcgggtg tgtgaagctc tcaatcagtg gttgcaggct
    16261 ggtaacaggc ttttccgtcg ggtgatgacg attgcctgaa tatttccagc ccagcacgtc
    16321 gggcaatggc ttctgaggca gcgccggacg cccttttgcc aggatgtaag cgcattcgtg
    16381 gcgatagccc acatatgcgg ccttcgaagt gtaggttttg gtaaacacca ggtggccaac
    16441 cacgctgaat cccgcatttt tccaggcggc cataaagcga tcgacgcggt tccagccata
    16501 gaagctcacc atcagcgcat cttttttcag cacgcggtac atttcattac aggccggttg
    16561 cagccattca tcggttttat cgccggcgat ggtacgcccc tgacggtcac ggaaaccgac
    16621 gagatatggc gggtcggtga ggatgaaatc gacaccattg cccggaatcg tggccataac
    16681 acgcacgcag tcaccctgga taaaacggga cataacagca ctccttctct tacagagggc
    16741 accgggaggg cgcccctgtg gcgcccgttc cccgcggcct gtgggctgtt caccgcgtcc
    16801 ggtaacgggt caggccgacc gtggaatatc gcagccgtcg tctttttgac ggcgagaata
    16861 tgccgcgaaa gcggcttgaa gggcgttcat cagaaaaggg cgacgggctg gcgtacactg
    16921 tcaccgatgc cagccgggag cggtaaaagc gcccgtcccg tgcccggccg gacgcgggac
    16981 actgaccaca ggcagcgttc ctgccgcgcc gcgtcagccg gcgcagtaaa aagaggagcc
    17041 cggcgacatc ctttaccgga aaagaaacgg gcggttgccc gcccgtctcc gtcatacttc
    17101 atccaccgta atgcgccacc ttgtcgacta tccactccgg cgcggtgact tccggcagct
    17161 catcatcatc tgtcggggaa gaccattcca gttcccccca cagcacatca acaagctctc
    17221 cacgctcata tcgctgccag ccacagaaat tgcagccctg cccggcgtac cagtgctcca
    17281 gcatgcaact gaaacggcgg cttaatactg caataacgtc gctttccggc tgacaccacg
    17341 gcgtgtcgaa atcaacctga atgaagttgt ctccctgact gctgatattc acctcatacc
    17401 cacaaggcca cttcacgcca tactgttccg tataccagtg atatgcagaa ggaacaccgt
    17461 tcagaacacc accgttaaag ccattcactt caacatccag gcgagtggac agaaccgtca
    17521 gcaggtcaaa cggcatggtt ttttccggca ggacgttgtc acacagacgg ttccaccaca
    17581 cagatacatc ctcgttactc cagatgtcgc accagtcacc tctttttggg gtgaaaagag
    17641 cggtaatgct ttccctggca tcatccggta attcttccca gcgacgccgg ccagtaccgc
    17701 tctgcagcca gagttcatgc agcatcaggc agttttgctc atccagaagt acaccgtcct
    17761 gcaggtgtgt cagccagcgg gtgaacgcga tattctccgg cgatacaacg ccacgcccgg
    17821 cagccgtcag tccggggcat ggttcaaacc gcacatcttc agtggtctgc agaagtccgg
    17881 cacttccggc cagaaacagc tgaatacctt catttgtggc gcggcgataa agcggtgtga
    17941 ctgcaccgct ggccagtcgt ttaatctcag cgatctgtgc
//
LOCUS       DAZFTN010000092        17911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFTN010000092 DAZFTN010000000
VERSION     DAZFTN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            244..969
                     /locus_tag="ACHKV2_003576"
     CDS             244..969
                     /locus_tag="ACHKV2_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6941391.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            927..1604
                     /gene="bglJ"
                     /locus_tag="ACHKV2_003577"
     CDS             927..1604
                     /gene="bglJ"
                     /locus_tag="ACHKV2_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO6941392.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1642..2430)
                     /gene="fhuF"
                     /locus_tag="ACHKV2_003578"
     CDS             complement(1642..2430)
                     /gene="fhuF"
                     /locus_tag="ACHKV2_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO6941393.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2571..2807
                     /locus_tag="ACHKV2_003579"
     CDS             2571..2807
                     /locus_tag="ACHKV2_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO6941394.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2846..2932)
                     /locus_tag="ACHKV2_003580"
     tRNA            complement(2846..2932)
                     /locus_tag="ACHKV2_003580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2896..2898),aa:Leu,seq:cag)
     gene            complement(3122..4153)
                     /gene="rsmC"
                     /locus_tag="ACHKV2_003581"
     CDS             complement(3122..4153)
                     /gene="rsmC"
                     /locus_tag="ACHKV2_003581"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO6941395.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4256..4669
                     /gene="holD"
                     /locus_tag="ACHKV2_003582"
     CDS             4256..4669
                     /gene="holD"
                     /locus_tag="ACHKV2_003582"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO6941396.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4638..5084
                     /gene="rimI"
                     /locus_tag="ACHKV2_003583"
     CDS             4638..5084
                     /gene="rimI"
                     /locus_tag="ACHKV2_003583"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6941397.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5099..5776
                     /gene="yjjG"
                     /locus_tag="ACHKV2_003584"
     CDS             5099..5776
                     /gene="yjjG"
                     /locus_tag="ACHKV2_003584"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO6941398.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5867..7456
                     /gene="prfC"
                     /locus_tag="ACHKV2_003585"
     CDS             5867..7456
                     /gene="prfC"
                     /locus_tag="ACHKV2_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO6941399.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7849..8454
                     /gene="osmY"
                     /locus_tag="ACHKV2_003586"
     CDS             7849..8454
                     /gene="osmY"
                     /locus_tag="ACHKV2_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO6941400.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8581..8742
                     /locus_tag="ACHKV2_003587"
     CDS             8581..8742
                     /locus_tag="ACHKV2_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO6941401.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8864..9937
                     /locus_tag="ACHKV2_003588"
     CDS             8864..9937
                     /locus_tag="ACHKV2_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO6941402.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9934..10716
                     /locus_tag="ACHKV2_003589"
     CDS             9934..10716
                     /locus_tag="ACHKV2_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6941403.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(10930..11793)
                     /locus_tag="ACHKV2_003590"
     CDS             complement(10930..11793)
                     /locus_tag="ACHKV2_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO6941404.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(11765..13314)
                     /locus_tag="ACHKV2_003591"
                     /pseudo
     CDS             complement(11765..13314)
                     /locus_tag="ACHKV2_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            13572..14351
                     /gene="deoC"
                     /locus_tag="ACHKV2_003592"
     CDS             13572..14351
                     /gene="deoC"
                     /locus_tag="ACHKV2_003592"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO6941405.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14478..15800
                     /gene="deoA"
                     /locus_tag="ACHKV2_003593"
     CDS             14478..15800
                     /gene="deoA"
                     /locus_tag="ACHKV2_003593"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO6941406.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15852..17075
                     /gene="deoB"
                     /locus_tag="ACHKV2_003594"
     CDS             15852..17075
                     /gene="deoB"
                     /locus_tag="ACHKV2_003594"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6941407.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17132..17851
                     /gene="deoD"
                     /locus_tag="ACHKV2_003595"
     CDS             17132..17851
                     /gene="deoD"
                     /locus_tag="ACHKV2_003595"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO6941408.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4329 a         4548 c         4794 g         4240 t
ORIGIN      
        1 taagtatttc taacaactta atgtagatat tttaaatgtc tccaggctat ttcaccaggt
       61 tatcatttga tgctaacctt ctgacacatg cagtggagtt gttgtgcagc aggagtatgc
      121 tgatatgaaa gtgaatgcta aggataattt attcgcttaa tctattaatt tgctggaaac
      181 atttaaggaa tgaatatcct gaataaacca tcataatttt tagacagtgg atgtggagga
      241 aatatgttgc caggatgctg caaaaatgga attgttatca gtaaaatacc tgttatgcaa
      301 gcagggttaa aagaggtcat gaggactcac ttccctgaat atgaaataat atccagcgcc
      361 tctgcggagg accttacctt attacaatta cgtcgttccg gattagtcat tgctgattta
      421 gccggtgaaa gtgaagatcc acgttctgtt tgtgaacatt attattcttt aatctcacaa
      481 tatcgggaaa ttcactgggt tttcatggtg tcacgctctt ggtattccca ggctgtagaa
      541 ctgctcatgt gccctacggc gacgttattg tctgatgttg aacccattga gaatctggtc
      601 aagaccgtac gttccggcaa tacgcacgca gagcgtatca gcgccatgct gacctctccg
      661 gcaatgactg aaactcatga ttttagctat cgctccgtca ttctcactct ttcagagcgc
      721 aaggtactgc ggctattagg taaaggatgg ggcatcaacc agatagcttc attgcttaag
      781 aaaagtaata aaactatcag cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac
      841 agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga acttaacttg
      901 ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag agaaatgtcg
      961 cactcataga aaaatgcgtc atgagtagta tcggtattga gagtttattc agaaagtttg
     1021 cgggcaaccc ttataagctc catacctata ccagtcagga gtcatttcag gatgccatgt
     1081 cgcggatctc gtttgcggcg gtcatttttt ctttttctgc catgagaagt gagcgcagag
     1141 agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg cgtttagtta
     1201 ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca ccgctggacg
     1261 gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt ttgtcattaa
     1321 atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac caaagccgga
     1381 cgttaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac tcaatgacac
     1441 aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa tttaatgtga
     1501 tgtcgaaact gggcgtcagt tctgacgcag ggttgttgga ggccgcagat attctgttat
     1561 gtatgcggca ttgcgaaaca agtaatgtgt tgcatcccta ttaatccgca tgatgccggg
     1621 tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct gtacatccgg
     1681 taagcgataa cgctggcagc aagtgcggcg caccagcagg ccgtcgcgta gcaccacggt
     1741 acgccagagt ggattatctt caccggtagt aagcgttttc tcaaaaaaga gggcatggcg
     1801 cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtaagat accagttgat
     1861 caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg cttctagtgc
     1921 ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct gcggcgaatg
     1981 tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcgacgc gtccggtttc
     2041 gtgaaactca gcatggaaat gttccggcga cacatctaat gctttttcct gcgtcagtag
     2101 cgccagcatt aatggtggca ccatcaggcc gatataccat tgtgcccata gtgagatcag
     2161 cggtttgtac tcgcggatca tcgttggttg gttgcgatag atatgatcgg aatagaccgc
     2221 cagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg
     2281 agcgggttca tccaggcgga taaactccag caaatgttca cgatgttcgg cgatcgtcgc
     2341 acgtacggct tgtgcaagcc tagcatcctg tggctggaga tgcgttcgcc agatgatatc
     2401 ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa tgataatgat
     2461 tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt gcaagtgata
     2521 aaaattatca tatgatattg gttatcatta tcaatgaaag agataaaacc atgttgcaac
     2581 gtacgctggg cagtggctgg ggagtgttgc tgccgggatt tctgattgca gggctgatgt
     2641 atgcggattt atcgccggat cagtggcgga ttgtcattct gatgggatta gtattgacgc
     2701 cggtgatgct gtatcacaaa cagttgcggc attacatttt gctaccatcg tgcctggcgc
     2761 ttattgctgg catcatgctg atgataatga atttgaatca gggatgaaaa aacagggaag
     2821 aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc cccacgtccg
     2881 taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcataccatc
     2941 aattcttaaa aagaattgct accacggagg cgcattctag tggttttcag cttttcgtca
     3001 atagttaatt atcgcctgat gtgtaattgc tggaaaaatg tccatcagga gactaacgtg
     3061 caggttttgt aagtcggata aggcgttcac gccgcatccg acacggtatt cggcgagata
     3121 attaaccttt cttcgcctgg cgggtcataa tggcgcgata caccttgaag cgccctgttt
     3181 gcgcgatcac ttcgtggaag ccaaatgtct catccagcac gtccggataa ggcaggaagg
     3241 cgttcgctac aattcgcagc tcgccgccgc tattaagatg acgcaccgca ccgcgaatca
     3301 gcgtttgcgc cgcatccagg ctggtttgca tcccatcgtg gaacggcggg ttggagatga
     3361 tcatatcaaa acgacctttc acctcggaaa agacgttgct ggcaaagact tcaccttcaa
     3421 caccgttggc cgcaagtgtt gcgcggctgg cttctaccgc tggcgcagag acatcgcaca
     3481 aggtgagacg aattttcggc gaatggcgcg caaaggcaac cgaaagcacg cccgcgccac
     3541 agccgacatc cagcacttta cctttcgtgt gcggggttaa cgtcgagagc agcaactggc
     3601 taccgacatc cagaccgtcg cggctaaaca cgccaggcag cgttttgacc gtcaggccat
     3661 cgacgctgta ttcgccccag aatttatcgg catcaaatac cggctgtttt tccagacgac
     3721 caaaatagag gccacagcga cgggcgctgt cgactttatt caacggcgca taatcagcca
     3781 gcatctgctc ggcgctgcgc acgccgctgc ggttctcgcc aacaacaaaa atatccgtcc
     3841 ccaccggaag cagagaaagt aaattcatca actggaactg ggcttccggt ttgttcttcg
     3901 gccagtagta aatcagcgta tcgcaatctg cgacatcatc cgccgtggcg accagactaa
     3961 aacgggcgtt atcccccatc tggcggctta acacctgcca atgctggaat tgctgggtat
     4021 gagcacggct ggccgcggta tctaaacgcg cgggcaggtc atcctgtaag tctccggcaa
     4081 acagaatacg gctttgttcg aaatcatcac tgtgacgcag caagacttca cttgccgggg
     4141 taaatgcaga catggaatgc tcctcaattg atactggcgg cgattatagc catatgttgg
     4201 cgcggtatcg acgaatttgc tatatttgcg cccctgacaa caggagcgat tcgctatgac
     4261 atcccgacga gactggcagt tacagcaact gggcattacc cagtggtcgc tgcgtcgccc
     4321 tggcgcgttg cagggggaga ttgccattgc gatcccggca cacgtccgtc tggtgatggt
     4381 ggcaaacgat cttcccgccc tgactgatcc tttagtgagc gatgttctgc gcgcattaac
     4441 cgtcagcccc gatcaagtgc tgcaactgac gccagaaaaa atcgcgatgc tgccgcaagg
     4501 cagtcgctgc aacagctggc ggttgggtac tgacgaaccg ctatcactgg aaggcgctca
     4561 ggtggcatca ccggcgctca ccgaattacg ggcaaaccca acggcacgcg ccgcgttatg
     4621 gcaacaaatt tgcacatatg aacacgattt cttccctcga aacgactgat ttaccagcgg
     4681 cttaccacat tgaacaacgc gcccacgcct ttccgtggag tgaaaaaacc tttgccagca
     4741 accagggcga gcgttatctc aactttcagt taacgcaaaa cggcaaaatg gcggcgtttg
     4801 cgattacgca agtggtgctg gatgaagcta cattgttcaa tattgcggtc gatcctgact
     4861 atcagcgtca gggattggga agggcgctgc tggaacatct gatcgacgaa ctggaaaaac
     4921 gcggcgtggc gacactatgg ctggaagtcc gtgcctcaaa tgctgccgcc attgccctgt
     4981 acgaaagttt aggctttaac gaggcgacga ttcgccgcaa ttactacccc accacggacg
     5041 gtcgcgaaga cgccatcatc atggcgttgc caatcagtat gtaagacaag gtggaataat
     5101 gaagtgggac tggattttct ttgatgccga tgaaacgctg tttacctttg actcattcac
     5161 cggcctgcag cggatgtttc ttgattacag cgtcaccttt accgctgaag attttcagga
     5221 ctatcaggcc gttaacaagc cactgtgggt ggattatcaa aacggcgcga tcacttcatt
     5281 acagcttcag cacgggcggt ttgagagctg ggccgaacgg ctgaacgtcg agccaggtaa
     5341 actcaacgaa gcctttatta atgcgatggc ggaaatctgc acgccgctgc cgggcgcggt
     5401 ttctctgctt aacgccattc gtggcaacgc caaaatcggc atcatcacca acggctttag
     5461 tgccttgcaa caggtgcgtc tggaacgcac gggcctgcgt gattacttcg atttgctggt
     5521 gatttccgaa gaagttggcg ttgccaaacc gaataagaaa attttcgatt atgcgctgga
     5581 acaggcgggc aatcctgacc gttcacgcgt gctgatggtt ggcgacactg ccgagtccga
     5641 tattctcggt ggcatcaacg ccgggcttgc gacctgctgg ctgaatgcac accatcgcga
     5701 gcaaccagaa ggcatcgcgc ccacctggac cgtttcttcg ttgcacgaac tggagcagct
     5761 cctgtgtaaa cactgattgc ctcccccccg ttgatgggta aaatagccgc aatttttcgt
     5821 tttcaacaag cgcggcgcga tgccgcttac tcaagaagaa agaattatga cgttgtctcc
     5881 ttatttgcaa gaggtggcga agcgccgcac ttttgccatt atttctcacc cggacgccgg
     5941 taagactacc atcaccgaga aggtgctgct gttcggacag gccattcaga ccgccggtac
     6001 agtaaaaggc cgtggttcca accagcacgc taagtcggac tggatggaga tggaaaagca
     6061 gcgtgggatc tccattacta cgtctgtgat gcagtttccg tatcacgatt gcctggttaa
     6121 cctgctcgac accccggggc acgaagactt ctcggaagat acctatcgta ccctgacggc
     6181 ggtggactgc tgcctgatgg ttatcgacgc cgcaaaaggt gttgaagatc gtacccgtaa
     6241 gctgatggaa gttacccgtc tgcgcgacac gccgatcctc acctttatga acaaacttga
     6301 ccgtgatatc cgcgacccga tggaattgct cgatgaagtt gagaacgagc tgaaaattgg
     6361 ctgcgcaccg atcacctggc caattggctg cggcaagctg tttaaaggcg tttaccacct
     6421 ttataaagat gaaacctatc tctatcagag cggtaaaggg cacaccattc aggaagtccg
     6481 cattgttaaa gggctgaata acccggatct cgatgctgcg gttggtgaag atctggcaca
     6541 gcagctgcgt gacgaactgg aactggtgaa aggcgcgtct aacgagttcg acaaagagct
     6601 gttccttgcg ggcgaaatca ctccggtatt cttcggtact gcgctgggta acttcggcgt
     6661 tgatcatatg ctggatggcc tggtggagtg ggcgcctgcg ccgatgccgc gtcagaccga
     6721 tacccgtacc gtagaagcga gcgaagataa atttaccggc ttcgtattta aaattcaggc
     6781 caacatggac ccgaaacacc gcgaccgcgt ggcgtttatg cgtgtggtgt ccggtaaata
     6841 tgaaaaaggc atgaagctgc gccaggtgcg cactgcgaaa gatgtggtga tctccgacgc
     6901 gctgaccttt atggcgggtg accgttcgca cgttgaagaa gcgtatccgg gcgatatcct
     6961 cggcctgcac aaccacggca ccattcagat cggcgacacc tttacccagg gtgagatgat
     7021 gaagttcacc ggtattccga acttcgcacc agaactgttc cgtcgtatcc gcctgaaaga
     7081 tccgctgaag caaaaacagc tgctcaaagg gctggtacag ctttccgaag agggcgcggt
     7141 gcaggtgttc cgtccaatct ccaacaacga tctgatcgtt ggtgcggttg gtgtgctgca
     7201 gtttgatgtg gtggtagcgc gcctgaagag cgaatacaac gttgaagcag tgtatgagtc
     7261 agtcaacgtt gccactgccc gctgggtaga atgtgcagac gcgaagaaat tcgaagagtt
     7321 caagcgtaag aacgaaagcc aactggcgct tgatggcggc gataacctcg cttacatcgc
     7381 taccagcatg gtcaacctgc gcctggcaca ggaacgttat ccggacgttc agttccacca
     7441 gacccgcgag cattaattct tgcctccagg gcgcggtagc cgctgcgtcc tgtcaatttc
     7501 ccttccttat tagccgctta cggaatgttc ttaaaacatt cacttttgct tatgttttcg
     7561 ctgatatccc gagcggtttc aaaattgtga tctatattta acaaagtgat gacatttctg
     7621 acggcgttaa ataccgttca atgcgtagat atcagtatct aaagccgtcg attgtcattc
     7681 taccgatatt aataactgat tcagaggctg taatggtcgt tattcatcac tcatcgcttt
     7741 tgtgatggcg accattgact tctgtagagg gtgaagtctc tccctattca gcaatgcaac
     7801 ctcgtgttgc caggctcaaa ttacgagcaa acatacagga ataaatcgat gactatgaca
     7861 agactgaaga tttcgaaaac tctgctggct gtaatgttga cctctgccgt cgcgaccggc
     7921 tctgcctacg cggaaaacaa cgcgcagact accaatgaaa gcgcagggca aaaagtcgat
     7981 agctctatga ataaagtcgg taatttcatg gatgacagcg ccatcaccgc gaaagtgaag
     8041 gcggcgctgg tggatcatga caacatcaag agcaccgata tctctgtcaa aaccgatcaa
     8101 aaagtcgtga ccctgagcgg tttcgttgaa agccaggccc aggccgaaga ggcagtgaaa
     8161 gtggcgaaag gtgttgaagg ggtgacctct gtcagcgaca aactgcacgt tcgcgacgct
     8221 aaagaaggct ctgtgaaggg ctacgcgggt gacaccgcca ccaccagtga aatcaaagcc
     8281 aaattgctgg cggacgatat cgtcccttcc cgtcatgtga aagttgaaac caccgatggc
     8341 gtggttcagc tctctggtat cgtagattct caggcacaaa gtgaccgtgc tgaaagtatc
     8401 gccaaagcgg tagatggtgt gaaaagcgtt aaaaatgatc tgaaaactaa gtaattcgtc
     8461 gtaattcgtc ctcccgaact ttgtcgggag gcgcgatgtg caccacacta aaaatatcgc
     8521 attgagtgat tttcacgctc atatcaagcg gtgacattaa ctatggtaaa ggagacgctt
     8581 atgtttcgtt ggggcatcat atttctggtt atcgcgttaa tcgccgccgc acttgggttt
     8641 ggtggtctgg ccggtaccgc tgcaggcgca gctaaaattg tctttgtcgt cgggattatt
     8701 ctgttcctgg tgagtttgtt catgggccga aaacgaccct agatttcaga ccattaagaa
     8761 aattttatcc aaagccagtc cagcggactg gcttttgcgg ttttagcgaa taataaattg
     8821 cgttactttg tcattctttc acaacaaaaa caggaaggca gaggtggggc agcgaatacc
     8881 tgtaacgctt ggtaatattg cgccgttgtc gctaaggccg ttccagcctg gacgaatagc
     8941 tctggtgtgc gaaggcggcg gacagcgtgg aattttcacg gctggcgtgc tggatgagtt
     9001 tatgcgcgcg cagtttaatc ctttcgatct ttatctcggc acatctgccg gggcgcagaa
     9061 cctctcggcg tatatctgca atcagcccgg ttacgcgcgc aaagtcatca tgcgctatac
     9121 cacaaaacgc gaatttttcg atccattgcg ctttgtccgt ggaggaaatc ttatcgatct
     9181 cgactggctg gtggaggcca ccgcaagcca gatgccgttg caaatggata ccgccgcgcg
     9241 gttgtttgac agcggcaaat cgttttatat gtgcgcctgt cgtcaggatg actacgcgcc
     9301 gaattacttt ttaccaacca aacaaaactg gctggatgtg attcgcgcct ccagtgcgat
     9361 acctggcttt tatcgtagcg gagtgtcgct ggaaggcatt aactacctgg atggcgggat
     9421 cagtgatgcg attccggtta aagaggcggc aaggcagggc gctaaaacgt tggtcgtcat
     9481 ccgcactgtg ccgtcacaaa tgtactacac gccgcagtgg ttcaaacgca tggaacgctg
     9541 gctgggtgac agtagcctgc agccgctggt caatctggtg cagcatcatg aaaccagcta
     9601 tcgtgacatt cagcaattta ttgagaaacc accgggcaag ctgcggatat tcgaaattta
     9661 tccgccgaag ccattacata gtatcgcgct tggcagtcgg attccggcgc tgcgtgaaga
     9721 ctataaactt gggcgtttat gcggtcgtta tttcctcgcc acggttggca agctattaac
     9781 tgaaaaagcg ccgcttaccc gccatctggt gccagtggtg acgccggaat cgattgtcat
     9841 tccgcctgcg ccagtcgcca acgatacgct ggttgccgaa gtgagcgacg ctccgcaggc
     9901 gaacgacccg acatttaaca atgaggatct ggcttgattt gccgttttat cgacacccac
     9961 tgccattttg atttcccgcc ttttattggc gatgaagagg ccagcctgca acgcgcggca
    10021 caagcgggcg taggcaagat cattgttccg gcaaccgagg cggaaaattt tgcccgcgtg
    10081 caggcgttag cggaaaatta tcaaccgctg tatgccgcat tgggcttgca tcctggtatg
    10141 ttggaaaaac atagcgatgt gtctcttgat cagctacagc aggcgctgga aaggcgtccg
    10201 gcgaaggtgg tggcggtggg ggagagcggt ctggatctct tcggcgacga tccgcaattt
    10261 gagaggcagc agtggtttct cgacgaacaa ctgaaactgg cgaaacgcta cgatctgccg
    10321 gtgatcctgc attcacggcg cacacacgac aaactggcga tgcatcttaa acgccacgat
    10381 ttatcgcgca ctggcgtggt tcacggtttt tccggcagcc tgcaacaggc cgagcggttt
    10441 gtacagctgg gctacaaaat cggcgtaggc ggtactatca cctatccacg cgccagtaaa
    10501 acccgcgttg tcatcgcaaa attaccgctg gcatcgttgt tgctggaaac cgacgcgccg
    10561 gatatgccgc tcaacggttt tcaggggcag cctaaccgcc cggaacaggc tgcgcgggtg
    10621 tttgacgtgc tttgcgagtt gcgcccggaa ccagaagatg agattgccga agtgttgctt
    10681 aataacacgt atgcggtgtt taacgttcgt gggtagtttg cccttcgtag gcagcatccg
    10741 gcagtcggcg cataatgcct gatgcgacgc ttaacgcgtc ttatcatgcc tacaggtttg
    10801 tgccgaaccg taggccggat aaggcgttca cgccgcatcc ggcagttggc gcacaatgcc
    10861 tgatgcgacg cttgacgcgt cttatcaggc ctacaagtct gtgccgaacc gtaggtcgta
    10921 tccggcatgt cacaaataca gcgccggaaa tattaaccgg ctcaccccgc gctcccgtaa
    10981 cgcatcagcc aacggctcaa cgtcttccgg cgtggcgctc gcccagcttt gcgcctcgcc
    11041 atacacgccg tgggcatgaa acgcgttcag gcgtaccgga acatcgccga ctcccttgat
    11101 aaacgccgcc agttcttcga tgtgttgcaa ataatccact tggccaggga ttaccagcaa
    11161 acgcagttcc gccagcttac cgcgctctgc cagcaaacag atgctgcgct taatctgctg
    11221 attatcgcgt ccggttaatt gctgatgaca ttcgctcccc cacgctttga gatcgagcat
    11281 tgcgccgtcg cacaccggga gcaatttttc ccagccggtt tcgctcaaca taccgttact
    11341 gtccaccagg caggtgaggt ggcgcagttg cggatcgttt ttgatagcag taaacagcgc
    11401 cactacaaac ggcagttggg tcgtggcttc gccgccactc accgttatcc cttcgataaa
    11461 cagcactgct ttgcggacat ggctaagcac ctcatccacg ctcatggatt gcgccattgg
    11521 cgtggcatgt tgcggacacc tcttcaggca ggtatcacat tgctcgcaga ccacagcgtt
    11581 ccacaccact ttgccgtcaa caatctgcaa cgcctgatgc ggacactgtg gcacgcactc
    11641 cccacagtca ttgcaacgtc ccatcgtcca cggattgtga cagtttttgc agcgcagatt
    11701 gcagccctgc aaaaacagag ccagacgact gcctggcccg tcaacgcagg agaaggggat
    11761 aatcttactg actaaagcgc atctgctgtt catggcttat cacgcgcggc tggcgttcca
    11821 gaatacgagt gttgcgtgcg gcttcttcgc ccagccaggt ggtgttggtg cgtgaacctt
    11881 cggcgcgata tttttctaaa tccgacaaac gcaccatata accggtaacg cgaaccagat
    11941 cgttaccgct gacattggcg gtaaattcac gcattccggc tttaaaggca ccgaggcaaa
    12001 gctgtaccaa cgcctgcggg ttacgtttga tggtttcgtc gagcgtcaga atgtcgctga
    12061 tgccagaata ataataagca tgatgcgggc gacagtttgc agatgggtga tcggatctgg
    12121 ctcatcgcca tacggcaaac gcgcgcccgg cgtggtgccg atatcggaac tgatccccga
    12181 ctgtgcgtgt aacatggcgc gtttttgcca gccatatttc acgggggtat tggcgacaaa
    12241 ctccgccagc tgtgcactga tgcgataacc tacttcattt gcggcggctt ctttaccgta
    12301 gcgcgcggca atcccttctt tttcacacag caagttaacc gcttccgcca gcccatacat
    12361 gccaaacatt ggcacaaaac gttcagggtt aatcagccct tctttcacca ggaagctatt
    12421 ctcaaagaag tgtgattgtt gatagaggaa ttcacaccgc gcatcgatga tggcgatctg
    12481 ctgctggcag tagtgcggta gagtgcgcgt aaagaagtca tccagcgatt cgctgcgctc
    12541 ggcaatggct ttcaggttaa ggcgtaccag cgtgctgcca ccacccgcca gcggcagtga
    12601 gttgtaacag ctcacaatcc cgtagccccc ttttgtgaaa attttatcat gcaccggacc
    12661 gttggcgatg tgcggtttgc tacattcaca gatgttcttc gccacttcca gcagcaggtc
    12721 atcaggggtg atttcaggat cgtagataaa ggtcaggttc ggtgaaacct gcttcaaatc
    12781 tgcgtctgca cgtaagatcg cgcgggtaat gggcgaatca gacgggccga tattggcgtg
    12841 cataaaggcg tctggcaggg ttctgtcgag gtaacgccag aaacgtttta ttcgaacatc
    12901 gatctcgtct tgtgttagaa ttataacata cggttgcaac aacgcatcca gttgccccag
    12961 gtagaccggc atcgatgtga ccgacggtac gtggtggtaa agaatggtca gcagagagag
    13021 tgcgtcatca agatctttcg cgccttccag ctccagccat tcggaaccgt tcgccagaaa
    13081 acgggcgtaa tcgggtaaga catagcgcgg tttgtacggc gcatgacctt caaacatatc
    13141 gcagattaca ccttcatcca gcgcgcggcg ggcttcggca ggaagctgtg ggtaaggcag
    13201 attgttttct gcttccagtg ccagaaaatg gcgcttctgc tccgggctaa gcactgggct
    13261 ggtgacaatt tgctggcaac gttgttgcag tgcattttca tgagaagtgg gcatcttctt
    13321 ttccttttat gccgaaggtg atgcgccatt gtaagaagtt tcgtgatgtt cactttgatc
    13381 ctgatgcgtt tgccaccact gacgcattca tttgaaagtg aattatttga accagatcgc
    13441 attatagtga tgcaaacttg taagtagatt tccttaattg tgatgtatat cgaagtgtgt
    13501 tgcggagtag atgttagaat actaacaaac tcgcaaggtg aattttattg gcgacaagcc
    13561 aggagaatga aatgactgat ctgaaagcaa gcagcctgcg tgcactgaaa ttgatggacc
    13621 tgaccaccct gaatgacgac gacaccgacg agaaagtaat tgctctgtgt catcaggcca
    13681 aaactccggt cggcaatacc gccgctatct gtatctatcc tcgctttatc ccgattgctc
    13741 gcaaaacact gaaagagcag ggcaccccgg aaatccgtat tgctacggta accaacttcc
    13801 cacacggtaa cgacgacatc gaaatcgcgc tggcagaaac ccgtgcggca atcgcctacg
    13861 gtgctgatga agttgacgtt gtgttcccgt accgcgcgct gatggcgggt aacgagcagg
    13921 ttggttttga cctggtgaaa gcctgtaaag aggcttgcgc ggcagcgaat gtactgctga
    13981 aagtgatcat cgaaaccggc gaactgaaag acgaagcgct gatccgtaaa gcgtctgaaa
    14041 tctccatcaa agcgggtgcg gacttcatca aaacctctac cggtaaagtg gctgtgaacg
    14101 cgacgccgga aagcgcgcgc atcatgatgg aagtgatccg tgatatgggc gtagaaaaaa
    14161 ccgttggttt caaaccggcg ggcggcgtgc gtactgcgga agatgcgcag aaatatctcg
    14221 ccatcgcaga tgaactgttc ggtgctgact gggctgatgc gcgtcactac cgctttggtg
    14281 cttccagcct gctggcaagc ctgctgaaag cgctgggtca cggcgacggt aagagcgcca
    14341 gcagctacta agtaagatgc tttacgcctg atgcgctgcg cttatcaggc ctacgagacg
    14401 tatctacccg taggccggat aaggcgtaga cgcatccggc aaaagccgcc tcatactctt
    14461 ttcctcggga ggttaccttg tttctcgcac aagaaattat tcgtaaaaaa cgtgatggtc
    14521 atgcgctgag cgatgaagaa attcgtttct ttatcaacgg tattcgcgac aacactatct
    14581 ccgaagggca gattgccgcc ctcgcgatga ccattttctt ccacgatatg acaatgcctg
    14641 agcgtgtctc gctgaccatg gcgatgcgag attcaggaac cgttctcgac tggaaaagcc
    14701 tgcatctgaa tggcccgatt gttgataaac actccaccgg tggcgtcggc gatgtgactt
    14761 cgctgatgtt ggggccgatg gtcgcagcct gcggcggcta tattccgatg atctctggtc
    14821 gcggcctcgg tcatactggc ggtacgctcg acaaactgga atccatccct ggcttcgaca
    14881 ttttcccgga tgacaaccgt ttccgcgaaa ttattaaaga cgtcggcgtg gcgattatcg
    14941 gtcagaccag ctcactggct ccggcggata aacgtttcta cgcgacccgt gatattaccg
    15001 caaccgtgga ctccatcccg ctgatcactg cctcgatcct ggcgaagaaa ctggcggaag
    15061 gtctggacgc gctggtgatg gacgtgaaag tgggtagcgg cgcgtttatg ccgacctacg
    15121 aactctctga agcccttgcc gaagcgattg ttggcgtggc taacggcgca ggcgtgcgca
    15181 ccaccgcact gctcaccgac atgaatcagg tgctggcctc cagtgccggt aacgcggttg
    15241 aagtacgtga agcggtgcag ttcctgacgg gtgaatatcg taacccgcgt ctgtttgatg
    15301 tcacgatggc gctgtgtgtg gagatgctta tctccggcaa actggcgaaa gatgacgccg
    15361 aagcgcgcgc gaaattgcag gcggtgctgg acaacggtaa agcggcagaa gtctttggtc
    15421 gtatggtagc ggcacaaaaa ggcccgaccg acttcgttga gaactacgcg aagtatctgc
    15481 cgacagcgat gctgacgaaa gcagtctatg ctgataccga aggttttgtc agtgaaatgg
    15541 atacccgcgc gctggggatg gcagtggttg caatgggcgg cggacgccgt caggcatctg
    15601 acaccatcga ttacagcgtc ggctttactg atatggcacg tctgggcgac caggtagacg
    15661 gccagcgtcc gctggcggtt atccacgcga aagacgaaaa cagctggcag gaagcggcga
    15721 aagcggtgaa agcggcaatt aaacttgccg ataaagcacc ggaaagcaca ccaactgtct
    15781 atcgccgtat cagcgaataa cggtatactg atctgatcat ttaaatttga agcactgagt
    15841 acggagaaca tatgaaacgt gcatttatta tggtgctgga ctcattcggc atcggcgcta
    15901 cagaagatgc agaacgcttt ggtgacgtcg gggctgacac cctgggtcat atcgcagaag
    15961 cttgtgccaa aggcgaagct gataacggtc gtaaaggccc gctcaatctg ccaaatctga
    16021 cccgtctggg gctggcgaaa gcacacgaag gttctaccgg tttcattccg gcgggaatgg
    16081 acggcaacgc tgaagttatc ggcgcgtacg catgggcgca cgaaatgtca tccggtaaag
    16141 ataccccgtc tggtcactgg gaaattgccg gtgtcccggt tctgtttgag tggggatatt
    16201 tctccgatca cgaaaacagc ttcccgcaag agctgctgga taaactggtc gaacgcgcta
    16261 atctgccggg ttacctcggt aactgccact cttccggtac ggtcattctg gatcaactgg
    16321 gcgaagagca catgaaaacc ggcaagccga ttttctatac ctccgctgac tccgtgttcc
    16381 agattgcctg ccatgaagaa actttcggtc tggataaact ctacgaactg tgcgaaatcg
    16441 cccgtgaaga gctgaccaac ggcggctaca atatcggtcg tgttatcgct cgtccgttta
    16501 tcggcgacaa agccggtaac ttccagcgta ccggtaaccg tcacgacctg gctgttgagc
    16561 cgccagcacc gaccgtgctg cagaaactgg ttgatgaaaa acacggccag gtggtttcgg
    16621 tcggtaaaat tgcagacatc tacgccaact gcggtatcac caaaaaagtg aaagcgactg
    16681 gcctggacgc gctgtttgac gccaccatca aagagatgaa agaagcgggt gataacacca
    16741 tcgtcttcac caacttcgtt gacttcgact cttcctgggg ccaccgtcgc gacgtcgccg
    16801 gttatgctgc gggtctggaa ctgttcgacc gccgtctgcc ggagctgatg tctctgctgc
    16861 gcgatgacga catcctgatc ctcaccgctg accacggctg tgatccgacc tggaccggta
    16921 ctgaccacac gcgtgaacac attccggtac tggtttacgg cccgaaagta aaaccgggct
    16981 cactgggtca ccgtgaaacc ttcgcggata tcggccagac tctggcaaaa tattttggta
    17041 cttctgatat ggaatatggc aaagccatgt tctgatggat ttgggcggag cgttgactcc
    17101 gcctttgtta tgtcacaaaa aggataaaac aatggctacc ccacacatta atgcagaaat
    17161 gggcgatttc gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcga agtatattgc
    17221 tgaaactttc cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac
    17281 cggtacttac aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg
    17341 ctccatctac accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg
    17401 ttcctgtggc gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc
    17461 ctgcaccgat tccaaagtta accgcatccg ttttaaagac catgactttg ccgctatcgc
    17521 tgacttcgac atggtgcgta acgcagtaga tgcagctaaa gcactgggca ttgatgctcg
    17581 cgttggtaac ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt
    17641 gatggaaaaa tacggcattc tcggcgtgga aatggaagcg gctggtatct acggcgtggc
    17701 tgcagagttt ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca
    17761 cgagcaaacc actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact
    17821 ggaatccgtt ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gattgtcgtg
    17881 ttaagccgga gtgggggact gctccggctt t
//
LOCUS       DAZFTN010000093        17389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFTN010000093 DAZFTN010000000
VERSION     DAZFTN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHKV2_003596"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHKV2_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO6941409.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2797)
                     /gene="hemX"
                     /locus_tag="ACHKV2_003597"
     CDS             complement(1574..2797)
                     /gene="hemX"
                     /locus_tag="ACHKV2_003597"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO6941410.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQADTPAAAPQGE"
     gene            complement(2819..3559)
                     /gene="hemD"
                     /locus_tag="ACHKV2_003598"
     CDS             complement(2819..3559)
                     /gene="hemD"
                     /locus_tag="ACHKV2_003598"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO6941411.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3556..4497)
                     /gene="hemC"
                     /locus_tag="ACHKV2_003599"
     CDS             complement(3556..4497)
                     /gene="hemC"
                     /locus_tag="ACHKV2_003599"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO6941412.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4884..7430
                     /gene="cyaA"
                     /locus_tag="ACHKV2_003600"
     CDS             4884..7430
                     /gene="cyaA"
                     /locus_tag="ACHKV2_003600"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO6941413.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7470..7790)
                     /gene="cyaY"
                     /locus_tag="ACHKV2_003601"
     CDS             complement(7470..7790)
                     /gene="cyaY"
                     /locus_tag="ACHKV2_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO6941414.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(7952..8094)
                     /locus_tag="ACHKV2_003602"
                     /pseudo
     CDS             complement(7952..8094)
                     /locus_tag="ACHKV2_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8253..8456
                     /gene="yifL"
                     /locus_tag="ACHKV2_003603"
     CDS             8253..8456
                     /gene="yifL"
                     /locus_tag="ACHKV2_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6941415.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8493..9317
                     /gene="dapF"
                     /locus_tag="ACHKV2_003604"
     CDS             8493..9317
                     /gene="dapF"
                     /locus_tag="ACHKV2_003604"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO6941416.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9314..10021
                     /gene="yigA"
                     /locus_tag="ACHKV2_003605"
     CDS             9314..10021
                     /gene="yigA"
                     /locus_tag="ACHKV2_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO6941417.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10018..10914
                     /gene="xerC"
                     /locus_tag="ACHKV2_003606"
     CDS             10018..10914
                     /gene="xerC"
                     /locus_tag="ACHKV2_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO6941418.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10914..11630
                     /gene="yigB"
                     /locus_tag="ACHKV2_003607"
     CDS             10914..11630
                     /gene="yigB"
                     /locus_tag="ACHKV2_003607"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO6941419.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11714..13876
                     /gene="uvrD"
                     /locus_tag="ACHKV2_003608"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11714..13876
                     /gene="uvrD"
                     /locus_tag="ACHKV2_003608"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO6941420.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13921..14823)
                     /locus_tag="ACHKV2_003609"
     CDS             complement(13921..14823)
                     /locus_tag="ACHKV2_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFO6941421.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14906..15670)
                     /gene="yigE"
                     /locus_tag="ACHKV2_003610"
     CDS             complement(14906..15670)
                     /gene="yigE"
                     /locus_tag="ACHKV2_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO6941422.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15832..15888
                     /gene="ysgD"
                     /locus_tag="ACHKV2_003611"
     CDS             15832..15888
                     /gene="ysgD"
                     /locus_tag="ACHKV2_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO6941423.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16040..16990
                     /gene="corA"
                     /locus_tag="ACHKV2_003612"
     CDS             16040..16990
                     /gene="corA"
                     /locus_tag="ACHKV2_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO6941424.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17015..>17389)
                     /locus_tag="ACHKV2_003613"
     CDS             complement(17015..>17389)
                     /locus_tag="ACHKV2_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941425.1"
                     /translation="SIRNIFYPSKLERLSRFQFIIYTLTSWSILITLNDINLALSFIL
                     VINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVA
                     WILFTTIMLSAIPSAKMSLSAH"
BASE COUNT         3991 a         4533 c         4628 g         4237 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacga
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg tgtcaggaag gtcccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc
      541 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tttgctggcg cagcactttt tccagctgtt ccggattgtt tgttttcagt
      661 cgaggaatcg gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta
      841 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct
     1141 gcgcgttcca gatgttggtt ggcgcgcgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt
     1321 gcacggcgac gcttacgccc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa tatgatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga
     1501 tgaccggcaa tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcagct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     1681 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     1741 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     1801 ggtctacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     1861 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttgctggtga cgcggtacag
     1921 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     1981 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2041 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2101 caccgtcgga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2161 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2221 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2281 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagcggtc gtgacgtcct
     2341 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2401 aggttttagc atcgctgccg gaaatggtgg cgaccttttg ttggacttca tccaactgtt
     2461 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg tgcagcttgt tgcttaataa
     2521 tgccttccag ctcgactttt tggctctcct gggctttttg caatgccgtc agttggttag
     2581 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     2641 cgacgcccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcggtattgt
     2701 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     2761 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     2821 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     2881 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     2941 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3001 cgctggtaac aacgaccgtc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3061 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgcg
     3121 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3181 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3241 gcgggtagag aatcttctgt ccgcttacgg tatgtagtgc cagtgcggtg gtgcgtccaa
     3301 tggcgaaata attaggtagt cggggccatt tacgatcttg ctgatgtagc tgtgattggg
     3361 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3421 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3481 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3541 gggtgacaag gatactcatg ccggggcgtc tccgttatag acttcagcga ggatctcgcg
     3601 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     3661 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcacg
     3721 cagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccacc
     3781 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     3841 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     3901 accttgtcct accgccggaa gagaaatctc gggtggcaac gcggcgcgaa tgcgggactc
     3961 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4021 cagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4081 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgccgg
     4141 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4201 acaaatagtg accaggccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4261 tacggcgata tcggcgcgat tttcgaggag cgcgacttcc agctctttta caaataagcc
     4321 ttttccgccg actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4381 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4441 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4501 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4561 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     4621 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     4681 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     4741 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     4801 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     4861 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     4921 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     4981 aggtctacag tctactgccg acattgttgc actatcacca tccgctaatg ccgggttacc
     5041 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5101 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggacccgccg aaaggtgagc
     5161 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5221 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttgc
     5281 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagttagct
     5341 tcttcctgat tgatgaaaac cgttttcgcc acaacgagag tggcagtctg gggggcgaag
     5401 attgtggctc cacccagcat atactgctgc ttgatgaatt ttatcgtacc gccgtgcgtc
     5461 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgatg
     5521 actacgtaat gacgctctac gcacaaggcg tgctgacgcc aaatgaatgg ctggatctcg
     5581 gcggcttaag ctcgctttcc gctgaagaat acttcggtgc cagcctttgg caactctaca
     5641 agagtatcga ttctccatac aaagcggtgc tgaagacact gctgctggaa gcctattcct
     5701 gggaataccc gaacccacgc ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     5761 agattgtatc gtttggcctc gatccttact gcatgatgct ggagcgtgtt actgaatacc
     5821 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     5881 tgtgcgaaaa gctcagccgt gaacgcgcct gcgtaggctg gcgtcgcgca gtgttgagcc
     5941 agttagtgag cgagtggggg tgggacgaag ctcgtctggc aatgctcgat aaccgcgcta
     6001 actggaagat tgatcaggtg cgtgaggcgc acaacgagtt gctcgacgcg atgatgcaga
     6061 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6121 aggatatcgg cgtgctgacg cgtaagctgt atgccgcgtt tgaagcatta ccaggtaaag
     6181 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6241 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6301 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6361 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg cttgtatatt aaaggtaacg
     6421 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgt
     6481 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc cgccatctgg
     6541 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     6601 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     6661 gcgttgacct gctgtatcgc aactcgtgga atgaagtgcg tacgctgcac ttcaacggcg
     6721 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     6781 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta attcgtactc
     6841 gcgtgcagca actggtttct gagtgtattg aattgcgtct ttccagcacc cgccaggaaa
     6901 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     6961 tgaatgtatc ggtacagaaa ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7021 acaaactgca cggcctgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7081 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagatgaga
     7141 atggctttaa tatctacatt ctcgacgaaa gcaatcgggt tgaggtgtat caccactgcg
     7201 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcatgacc
     7261 gttttaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7321 aggttgatgg tcgtgaacag gtgattccgt tccgcacaaa atctatcggt aacatgccgc
     7381 ctgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgaacgtgcc
     7441 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgc
     7501 tgcgtcgccg cctgttccag caaatcccgg aaggtttcgc cgctgcgatc gcaaatccac
     7561 tcatcgcctt tcagatcaaa atggtagccg ccttgtttgg ttgccagcca tacctggtgc
     7621 agcggttcct ggcggttgat aatgattttg ctgccattct caaaggtaat ggtcagcacg
     7681 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc agtcgtccag acgctcttca
     7741 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     7801 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     7861 ctgcggctgc gttacggcat gattcatccg tcaccggaat aatgatgtct ctgtgtagcg
     7921 aaagatttgt ctcttcatta gggcgcagtt acaccacgcc tttccctgtt tctggtaaac
     7981 attatgatca ggtttaccga gcgagcatcc tcacgctgac ggaactaaaa aagccaacaa
     8041 acaaaccaca ttgcgatagt gcataaagcc atcctggcgt gaggtgttga tcacgaaact
     8101 gccagcagaa cataaatcct cacgagtaag cgttatactc gcagcatttc ctcacttttc
     8161 agacttcata aagagtcgct aaacgcttgc ttttacgcct tctcctgcga tgatagaaag
     8221 cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag gcactcactg
     8281 tattacttac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc tatttcccgc
     8341 ctgcagataa aaatgcaccg ccgccgacca aaccggtaga gacgcaaacg caatccacgg
     8401 tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat tactaaaagt
     8461 cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg catggccttg
     8521 gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca ccggagctga
     8581 ttcgtcgcct agctgatcgg cacctggggg tagggtttga ccaactgctg gtggttgagc
     8641 cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat ggcagtgaag
     8701 tggcgcagtg cggcaacggc gcacgctgct ttgcccgttt tgtgcgtctg aaagggctga
     8761 ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg gatggttctt accgtcaccg
     8821 atgatgatct ggtccgcgta aatatgggcg aacccaactt cgaaccttct gccgtgccgt
     8881 ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag acaatcttat
     8941 gcggcgtggt gtcgatggga aatccacatt gcgtgattca ggtcgatgat gtcgatactg
     9001 cggcggtaga aacactgggg cctgttctgg aaagccacga gcgttttccg gagcgcgcca
     9061 atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt tatgagcgtg
     9121 gggcaggaga aacccaggcc tgcggcagcg gcgcatgtgc ggcggttgca gtagggattc
     9181 agcaaggttt gctggccgaa gaagtacgcg tggaactccc aggcggtcgt cttgatatcg
     9241 cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat gtctacgacg
     9301 gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca cggagcttga
     9361 tgaccgggtg gttgtcgatt atctgattaa aaatcctgag ttttttatcc gcaatgcgcg
     9421 cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggt accgtttcgt tggtcgagtg
     9481 gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg cgctgttgat
     9541 ggaacaggct atcgccaacg aaggcctgtt ttatcgctta ctctacctgc agcgcagtct
     9601 caccgccgcc agcagtcttg atgatatgct gatgcgcttt caccgctggg cgcgtgatct
     9661 cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag gtgcgccgtc
     9721 gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta ttcagcgtct
     9781 ggggcaggag cagcattatc tggggccgct taacggaccg gagctgctgg tggtactacc
     9841 ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg ctgatttagg
     9901 cgtggtgctg ttcaccagcc gcgatgccag tcactatcaa caagggcagg gaacgcagtt
     9961 acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg aacgcgtatg
    10021 accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga gcggcagctt
    10081 agtccgataa ctctgcttaa ctaccagcga cagcttgagg cgatcatcaa ttttgccagc
    10141 gaaaacggcc tgcaaaactg gcagcaatgc gatgcagcga tggtgcgcaa ttttgctgta
    10201 cgcagtcgcc gtaaagggct gggcgcggca agcctcgcat tacgcctctc tgcgctacgt
    10261 agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc gaaaggtgtt
    10321 tcggcaccga aaacgccacg tcatctgccg aaaaatatcg acgtcgatga tataaatcgc
    10381 ctgttggata ttgatatcaa cgatcccctc gctgtgcgcg accgtgcaat gctggaagtg
    10441 atgtacggcg cgggcctgcg tctttccgag ctggtggggc tggacatcaa acacctcgac
    10501 ctggagtccg gcgaagtgtg ggtgatgggg aaaggcagca aagagcgccg cctgccgatt
    10561 ggtcgtaacg ctttgtcatg gattgagcac tggcttgatt tgcgcgacct gtttggtagc
    10621 gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg taatgtgcag
    10681 aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca cccgcataaa
    10741 ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg tggtgtgcag
    10801 gagctgctgg gtcatgccaa cctctccacc acgcaaatct atactcatct tgattttcaa
    10861 caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa ataatgcgtt
    10921 tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat accctttacg
    10981 ataaccgtcc ggtgattttg cgcaccgagc aggaggcgct tacctttgtg caaaattacc
    11041 atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag gcggtacggg
    11101 aagcggaacc cgagatttat cacgacgtga cgcgctggcg ttttcgctca atagaacaag
    11161 cgatgcttga cgccggattg agcgcggaag aagccagtgc aggcgcacac gcggcaatga
    11221 tcaactttgc taaatggcgc agcaggatcg acgtcccgca gcaaacccac gacaccttaa
    11281 aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgct cagccggaac
    11341 tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac gggcgctcga
    11401 aaccgttcag cgatatgtac ttcctggcgg cggaaaaact caacgtgccg atcggcgaga
    11461 tcttacatgt aggtgacgat ctcaccactg acgtgggtgg ggcaattcgc agcggaatgc
    11521 aggcctgttg gatcagaccg gaaaatggcg atctgatgca aacctgggat agccgtttac
    11581 tcccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa tcagcaaatc
    11641 tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa cctattttta
    11701 cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca aacagcgcga
    11761 agcggtggcc gcgccgcgca gcaaccttct ggtgctggcg ggcgcgggca gtggtaagac
    11821 gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct cgccatactc
    11881 gattatggct gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc gtatcgggca
    11941 actgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc tggcgcaccg
    12001 tttgctgcgt gcgcaccata tggacgccaa tctgccgcag gatttccaga tcctcgatag
    12061 cgaagaccag ctacgcctgc ttaagcgtct gatcaaagcc atgaacctcg acgagaagca
    12121 gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag gcctgcgtcc
    12181 gcatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg tgtatcaggc
    12241 gtatcaggaa gcgtgcgatc gtgcaggcct ggtggacttc gccgagctgc tgctgcgcgc
    12301 tcacgagttg tggcttaaca agccgcatat cctgcaacac taccgcgaac gttttaccaa
    12361 catcctggtg gacgaattcc aggataccaa caacattcag tacgcgtgga tccgcctgct
    12421 ggcgggcgac accggcaaag tgatgatcgt cggtgatgac gaccagtcaa tctacggctg
    12481 gcgcggggcg caggtggaga atattcagcg tttcctcaat gatttccccg gtgccgaaac
    12541 tatccgtctg gagcagaact atcgttctac cagtaacatt ctgagcgccg cgaacgccct
    12601 gattgaaaac aacaacgggc gtctgggtaa aaaactgtgg accgatggcg cggacggtga
    12661 gcctatttcc ctctattgcg cttttaacga actcgatgaa gcgcgttttg tcgttaaccg
    12721 catcaaaacc tggcaggaca acggtggggc gctcgccgag tgcgccattc tctaccgcag
    12781 caacgcccag tcgcgggtgc tcgaagaggc gttattgcag gccagtatgc cgtaccgtat
    12841 ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gatgcgctct cgtatctgcg
    12901 cctgattgcc aaccgcaacg acgacgcggc ctttgagcgt gtggtgaata cgccaacgcg
    12961 gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc gccagttaac
    13021 actctggcag gcatgtcgtg aactgttgca ggaaaaagcc ctcgccggac gtgctgccag
    13081 cgccttacag cggtttatgg aactgatcga cgccttagcg caggaaactg ccgatatgcc
    13141 gctgcatgta cagactgacc gggtaattaa agactccggc ctgcgtacca tgtacgagca
    13201 ggagaagggc gaaaaaggtc agacgcgtat cgaaaactta gaggaactag tgacggcaac
    13261 gcgccaattc agctacaacg aagaagacga agatttaatg ccgctgcagg cgttcctctc
    13321 ccatgcggcg ctggaagcgg gcgaggggca ggcggatacc tggcaggatg cagtgcagtt
    13381 gatgacgctg cactcggcga aaggcctgga gttcccgcag gtgtttatcg tcggtatgga
    13441 agagggcatg ttcccaagcc agatgtcgct ggatgaaggc ggacgtctgg aagaagaacg
    13501 ccgtctggcc tacgttggtg taacccgcgc gatgcagaaa ctgacgctga cctacgcgga
    13561 aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta tcggtgagtt
    13621 gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc cggtcagcca
    13681 tcagcgtatg ggtacgccga tggtcgagaa cgacagcggc tacaagctcg gccagcgtgt
    13741 acgccacgct aagtttggtg aaggcactat cgtcaatatg gaaggcagcg gcgagcatag
    13801 ccgtttgcag gtggcattcc agggccaggg tattaaatgg ctggtggcgg catacgcccg
    13861 gctggagacg gtataacgtt gcgggatgcg gtgcgccaca ccgcatccgg catcagcacc
    13921 ttacttatca catttcttca catcaatatt ccagtcagca ccagacgcaa cctgatgttt
    13981 ctgcgcgaaa ttatccatat tcagcgccac ggaaacattc aataagctgt tcagatacac
    14041 catcggctgt ccttccggca catcgccgaa gcttgcgaca tacggcattt tccctgcgta
    14101 tttttgccgg gaaccttcgg aaatggtcac acacaacgtg tcgttgagtt tgatccccgc
    14161 ctgattcagc aactcgtcgc tgatattgct ccagacgttg ccgtactgaa tatcgagaat
    14221 cgggatattg cccttcactt caccttttgt tgcttttgct ttctgataag aaagttcgac
    14281 aacctttgca ggaagctcag gccccacctg ctcgaacgtg atcgccccgg aagccagacg
    14341 tgcaccggtg taggcataca cgtcacgacc gtggaaggta tatgactttt cagaaccttt
    14401 caggcggtta gctttttcgt cgatttcccg gacgctctca atacccagcg attccgccac
    14461 caaagtgagc gtaccgttat ccggtgaaac gaagtattga ccgtttttgg ttttcagcac
    14521 tatcgattta cggtcagtgc ctacgcccgg atcgaccaca ctgacaaata ccgaaccttg
    14581 cggccagtaa ctggcggtct gatacaggcg gtaagcgcct tcccagatgt tatacggtgg
    14641 aatttcgtgt gttaaatcaa atattttaag attatggtca acaccgaatg cgaccccttt
    14701 catcgccgag acggcaccat ctttcaggct aaaatcagtt tgtaaaatca aagcgttatc
    14761 cgcataagcg gatgatgatc ccagacacgt taataaaaat aacgttgcga gcgttttttt
    14821 catttttgtt tcccttcatt tatctgatga ttttccagct gtgtagccag ccccagtttg
    14881 tctggcctcc tcgagtgtac aatgcttaac ctttccgctc gacggaaatc atggtgacaa
    14941 aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa atcgtgccat
    15001 caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca aaatcataaa
    15061 aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta attccaacac
    15121 cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt acactgtttt
    15181 ccatcaacat tggccctgac tgcaccgcaa actgaatctc tttactggtt ttgaaggcat
    15241 ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca ggacggataa
    15301 agaaattccc ttcacctgaa gcgagattta acgccacctt ctgctgaccg ttttcgatgt
    15361 acaaaccgag cggcgcatag ctttcatcat agatgccgcc gttcatcgcc atctgcacct
    15421 gaccctgact attcatatcc gccagcagag catgtaaagt tccccacgct tcgccattgg
    15481 ctttttgcca gtacattttc acccgctctg tttgaggatt aacggtatac gcctgtacgg
    15541 tcagcgttgg atctgagagt gcgcaatcat cagcggcaac agcaaacaag ggaagtaagg
    15601 tgagggcgag aaaaatccgt ttgagattca aggtgatcat tcctttacca atgagtagcc
    15661 gatgcgccat tataggtcct ggatgtggga tttttttatc ctgttagcga ccttgacgag
    15721 taccaaaaag cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg ttgacggcaa
    15781 aattttgctg gcgtaacatg cgcgcacgat cactctaaga ggacattcgc cttggacaca
    15841 cccagtagat actggctcac tatcctgtca tccaggatca actcctaagg ctatcccttt
    15901 ttgctgatag ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc tgagtcaggc
    15961 tgtttaatgg tctgaaaccc aatttgtttc tgtgtgccca ccgaactgtc cgatatttta
    16021 agcattggga gtcccggtca tgctgagcgc atttcaactg gaaaataacc gactgacccg
    16081 gctggaagtc gaagagtcac aaccccttgt aaatgcagta tggattgatc ttgtcgaacc
    16141 ggacgacgac gagcgactgc gcgtacaatc tgaacttggc cagagcctgg caacccgccc
    16201 ggaactggaa gacatcgaag catcggcacg tttctttgaa gacgatgacg gcctgcatat
    16261 tcactccttc ttcttctttg aagatgcgga agatcacgcc ggtaactcca ctgtggcatt
    16321 taccatccgt gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg cttttcgtct
    16381 gtatcgtatg cgtgcccgta gccagtcgat ggtagacggt aacgcctacg agttgctgct
    16441 ggatctgttc gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata tctatagcga
    16501 cctggagcaa ttgagccggg tgattatgga agggcatcag ggcgatgagt acgacgaggc
    16561 gctctccact ctggcggaac tggaagatat cggctggaaa gtgcgcctgt gtctgatgga
    16621 tacccagcgc gcgctcaact tcctggtacg taaagcacgc ttaccgggtg ggcaactgga
    16681 gcaggcgcgt gaaatcctgc gagatatcga atccctgctg ccgcataacg aatccctgtt
    16741 ccagaaggtg aacttcctga tgcaggcggc aatgggtttt atcaacatcg agcagaaccg
    16801 catcatcaaa attttctcgg tggtatccgt cgtgttcctg ccgccaacgc tggttgcttc
    16861 cagctacggg atgaactttg agtttatgcc agaactgaag tggagtttcg gctacccagg
    16921 cgcgattatc tttatgatcc tcgcgggcct ggcaccgtat ctgtacttta agcgcaagaa
    16981 ctggctgtaa ctatacgatg tcgctgtgag taagttagtg tgctgacagc gacattttag
    17041 cagagggaat cgcagataac ataattgttg tgaataatat ccatgccacg ttgagttgga
    17101 cgcttaatga ctcacatctc accccttcaa ttgctgcaga gataatagaa aaaagtgtca
    17161 aacccaatga aatcaataca aagtagattg ccttacatcc agtatctctt attctttttg
    17221 ctccaatatt gatatttaca taggtattat taataaccag aataaaactc aaagccaggt
    17281 tgatatcatt caaagttatt aatattgacc atgatgttaa tgtatagatt atgaactgaa
    17341 agcgtgataa acgctcaagc ttggatgggt agaatatatt tctgatgga
//
LOCUS       DAZFTN010000094        17300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFTN010000094 DAZFTN010000000
VERSION     DAZFTN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..823
                     /locus_tag="ACHKV2_003614"
     CDS             29..823
                     /locus_tag="ACHKV2_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6941426.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(894..1343)
                     /gene="rplI"
                     /locus_tag="ACHKV2_003615"
     CDS             complement(894..1343)
                     /gene="rplI"
                     /locus_tag="ACHKV2_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO6941427.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1385..1612)
                     /gene="rpsR"
                     /locus_tag="ACHKV2_003616"
     CDS             complement(1385..1612)
                     /gene="rpsR"
                     /locus_tag="ACHKV2_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO6941428.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1617..1931)
                     /gene="priB"
                     /locus_tag="ACHKV2_003617"
     CDS             complement(1617..1931)
                     /gene="priB"
                     /locus_tag="ACHKV2_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO6941429.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1938..2333)
                     /gene="rpsF"
                     /locus_tag="ACHKV2_003618"
     CDS             complement(1938..2333)
                     /gene="rpsF"
                     /locus_tag="ACHKV2_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO6941430.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2660..2935
                     /gene="yjfY"
                     /locus_tag="ACHKV2_003619"
     CDS             2660..2935
                     /gene="yjfY"
                     /locus_tag="ACHKV2_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO6941431.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3064..3750)
                     /gene="ulaF"
                     /locus_tag="ACHKV2_003620"
     CDS             complement(3064..3750)
                     /gene="ulaF"
                     /locus_tag="ACHKV2_003620"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO6941432.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3750..4604)
                     /gene="ulaE"
                     /locus_tag="ACHKV2_003621"
     CDS             complement(3750..4604)
                     /gene="ulaE"
                     /locus_tag="ACHKV2_003621"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO6941433.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4614..5264)
                     /gene="ulaD"
                     /locus_tag="ACHKV2_003622"
     CDS             complement(4614..5264)
                     /gene="ulaD"
                     /locus_tag="ACHKV2_003622"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6941434.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5278..5742)
                     /gene="ulaC"
                     /locus_tag="ACHKV2_003623"
     CDS             complement(5278..5742)
                     /gene="ulaC"
                     /locus_tag="ACHKV2_003623"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO6941435.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5752..6057)
                     /gene="ulaB"
                     /locus_tag="ACHKV2_003624"
     CDS             complement(5752..6057)
                     /gene="ulaB"
                     /locus_tag="ACHKV2_003624"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO6941436.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6073..7470)
                     /gene="ulaA"
                     /locus_tag="ACHKV2_003625"
     CDS             complement(6073..7470)
                     /gene="ulaA"
                     /locus_tag="ACHKV2_003625"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6941437.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7825..8889
                     /gene="ulaG"
                     /locus_tag="ACHKV2_003626"
     CDS             7825..8889
                     /gene="ulaG"
                     /locus_tag="ACHKV2_003626"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO6941438.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8997..9752
                     /gene="ulaR"
                     /locus_tag="ACHKV2_003627"
     CDS             8997..9752
                     /gene="ulaR"
                     /locus_tag="ACHKV2_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO6941439.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9749..10498)
                     /gene="yjfP"
                     /locus_tag="ACHKV2_003628"
     CDS             complement(9749..10498)
                     /gene="yjfP"
                     /locus_tag="ACHKV2_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6941440.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10680..11009
                     /gene="bsmA"
                     /locus_tag="ACHKV2_003629"
     CDS             10680..11009
                     /gene="bsmA"
                     /locus_tag="ACHKV2_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO6941441.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11158..11433
                     /gene="yjfN"
                     /locus_tag="ACHKV2_003630"
     CDS             11158..11433
                     /gene="yjfN"
                     /locus_tag="ACHKV2_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO6941442.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11550..13175)
                     /locus_tag="ACHKV2_003631"
     CDS             complement(11550..13175)
                     /locus_tag="ACHKV2_003631"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO6941443.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13259..14422)
                     /locus_tag="ACHKV2_003632"
     CDS             complement(13259..14422)
                     /locus_tag="ACHKV2_003632"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6941444.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14425..15063)
                     /locus_tag="ACHKV2_003633"
     CDS             complement(14425..15063)
                     /locus_tag="ACHKV2_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO6941445.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15073..15471)
                     /locus_tag="ACHKV2_003634"
     CDS             complement(15073..15471)
                     /locus_tag="ACHKV2_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO6941446.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15489..16148)
                     /locus_tag="ACHKV2_003635"
     CDS             complement(15489..16148)
                     /locus_tag="ACHKV2_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO6941447.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16199..16897)
                     /locus_tag="ACHKV2_003636"
     CDS             complement(16199..16897)
                     /locus_tag="ACHKV2_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO6941448.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16916..>17300)
                     /locus_tag="ACHKV2_003637"
     CDS             complement(16916..>17300)
                     /locus_tag="ACHKV2_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO6941449.1"
                     /translation="ALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4405 a         4604 c         4142 g         4149 t
ORIGIN      
        1 ataaaaacat taaacaggtc acattacaat gacattacca accactattt attcatttcc
       61 tgcatattta tctcgttttt cgtccaccga caaaccagta aaattaaaat ttcatcaata
      121 cgcacgcgct acattacttt caaatcgtgg aagagatcat aattgcgatg gcagacgtac
      181 tgttgagatc cacaaactag atctctcaga ctggcaagca ttcaataagt tagcaacgaa
      241 gtgtaatgct tacgatggag taacaatgaa tggtgataat tcttttggat ggaaccatga
      301 agctacatta gataatattc acgcccagaa atacaataaa gcttacgcgg gtgcgaggtt
      361 aaccgcagaa ctgaaatatt tattgcaaga tgtcgaatcc ttcgaaccca acagtaaata
      421 cacaatacac gaagtggttt tgggacctgg ttatggtact ccagactata ccggccaaac
      481 gataggatac gttgtaaccc ttcctgccca aatgcctaat tgctggtcat ctgaactacc
      541 aacaatcgat ctttatatcg accaacttcg cactgttaca ggcgtttcta atgccctcgg
      601 attcatcatt gctgcattgc taaacgcgta ttctgattta ccccatgatt tgaaaattgg
      661 tctgcgttcc ttatcaagtt cagccgcaat ctactcgggt ttgggtttcg agcgagtgcc
      721 gcaggagcga gacatcagtt gcgctcgtat gtatctaacg ccagcaaacc atcctgactt
      781 atggacacaa gagaatggtg aatggatcta cttgcgaaac taatacctga cgaaaaatag
      841 aaagcaaaac gccgaccagt ggtcggcgtt tttacgtctc gttgaataac gaattattca
      901 gctactacgt ttacgatcac tttcgcgaat acttcgctgt gaacctggaa gctcacttcg
      961 tgttcgccag tggtacgcag aacgccgttc ggcagacgaa cttcgctctt agccacttca
     1021 acgccagctg cagttacagc gtcagcgatg tcgcgagtac cgatggaacc gaacagttta
     1081 ccttcgtcgc cagctttaga cgcgatggta acagtttcca gtgcattgat tttctcagcg
     1141 cgagcattag cagctgccag aacttcagcc agtttagctt ccagttcagc gcgacgtgct
     1201 tcgaagaatt caatgttttt cttggtagct ggaacagctt taccctgtgg taccaggaag
     1261 ttacgagcat agcccgcttt aacgtttacc tgatcaccca ggctacccag gtttgctact
     1321 ttatcaagca gaataacttg cattacctta tcctctcaaa gtcgtattaa tggaccgtga
     1381 ccgattactg atggcgatca gtgtacggca gcagggacag gtagcgagcg cgtttgatag
     1441 cgcgagccag ctgacgctgg tattttgcac gggtaccggt gatacggctt gggacaatct
     1501 taccgctttc ggtgatgtag tttttcagcg tagcgatatc tttatagtcg atctcttgaa
     1561 cgccttccgc ggtgaaacgg cagaacttgc gacgacggaa ataacgtgcc atatggctag
     1621 tctccagaat ctatcaattc aatctgctcg gcatgcaaaa ccattttgct cagtccgttc
     1681 tttgccttgt ggcatgaaat gaacccctga acggttatgc gactgccgac cgttatactg
     1741 tgagtaatgg cctggttttc gtgtccgcta acaataacgg gcatttgaca ccatgcctgc
     1801 cggtgaaagc cggcttcctc ctgcacagaa cgatgctcaa gcacgaactg gcagtgagga
     1861 attcctgatg gactgacctt tcgaagggga gtcctgcaca cggtgccgga caacaccaga
     1921 cggttggtca tcagaaatta ctcttcagaa tccccagctt cagcatcatc agcggtttcg
     1981 tttgcgaaat catcgcgacg ctcacggcgc tcgtctttcg ctttaaccat cggagatgct
     2041 tcggtaacag cgtgcttggt acgcataacc atgctgcgga taacggcatc gttgaagcgg
     2101 aaggtagttt ccagctcatc gatcacttcc tgcggagctt caacgttcat cagaacgtag
     2161 tgagctttgt gcagtttgtt gatcgggtaa gccagctgac ggcggcccca gtcttccaga
     2221 cggtggatct tgccttctgc accagtgatg gcagcagtgt agcgctcgat catgcccgga
     2281 acctgttcgc tctggtcagg atggaccata aaaacgattt cgtaatgacg catcgaattg
     2341 ctccttacgg attattcagc ctcctgtctg ggtcagccga atcccgggga ggcaaggaac
     2401 gtgttaaagg tcggctgaaa aatgacgcgt tatagtactg acataccccg ctaaactcaa
     2461 ggccagccga caaataaatc tcatctttca ccaaagtgga ctaacacgct gatttgaagt
     2521 tcatcacact tcatcatggt gttttttcga acaactgcat cagaaatggt caccatcaac
     2581 ttctttatca ttgattaaaa ttaaatcaga agatcatcac agaggagagc acacaaagtc
     2641 agccgtcgag gaggtatcta tgttcagtcg agttttagcc cttctggctg tgcttttgct
     2701 aagtgcaaat acatgggcag ccattgaaat taataaccac caggccagaa atatggacga
     2761 tgtgcaaagc ttaggcgtga tttatatcaa tcataatttc gccactgaaa gcgaagcacg
     2821 tcaggcatta aatgaagaaa cagatgcgca aggcgcaacg tactaccacg taattctgat
     2881 gcgggaaccg gggagtaacg gcaatatgca cgccagcgcg gatatttatc gctagcacca
     2941 ggtataacca acgaaacatt gccatagttt gctttgcccc cttcgcaggg ggcttttttt
     3001 gaaaaacggc tgcggaatta gaccagttat ctcccgagga aggaaatttc cgcagcgcgt
     3061 gttctacttc tgcccgtaat aagcgttggg gccgtgctta cgcatgaagt gtttattcat
     3121 caggaagctg tcgatgtgat tgagttgtgg attaatactg cgcgcaatcc acgccatttt
     3181 cgccacttct tccatcacta ccgcgttatg caccgcgtcg tgggcatctt tcccccaggc
     3241 gaacggtccg tgctgataca ccacaattcc cggcgtatgc agcggctcgg cgttgcccag
     3301 cgtttcgata atcactttgc cagtgttcag ttcatactca ccctgcacct cttcttcgct
     3361 taatccacgc gtacacggga tatcaccaaa gaagtagtca gcgtgcgtgg tgcctaacgc
     3421 cgggatcgcc agccccgcct gcgcccatgc ggtagcatga gtggagtggg tatggacaat
     3481 gccgccaagc gacggataac gacggtagag ttcgagatgc gtcgcggtgt cggaagacgg
     3541 acgatattcc ccttccacca ccttaccgct catatcgacc accaccatat cgtctgcttt
     3601 catggtttcg taggcgacgc cgctgggctt gatcaccacc agcccgcgtt cgcggtcgat
     3661 agcgctgacg ttgccccagg taaaggtcac cagcccatag cgcggcagat ccatgttggc
     3721 ttcaaacacc tgctgtttta gcttttgcat tatgccgcct ccaccatgcc cgctttcgcc
     3781 atgcgcgctt tcacccaatc acgcgctttc gccacttctg ccgccgggtc ttccgccgtt
     3841 tcgctccaca tctcaatcag gtacggcccg caatagccac tctgtttgag cgtttcgaag
     3901 caacgttcga aatccaccac accttcgcca aatggcacgt ttttgaagac gcccggtttg
     3961 gtgtctttca catgtaccgc gacgatatgc ccgattccgg cctgcaactc catctgcaca
     4021 tcgttgtccc acgccgacag gttgccgata tccgggtaga gctggaacca cggattgttg
     4081 agatagtgcg cgtagcccag cgccttgctg atggagttca tcaacggata atccatgatc
     4141 tccatcgcca gcgtcacctg cgcgcggctc gccatctcaa cgctctcttt caggccgtca
     4201 cggaaacgac gacgcgtttc gttattggct tcctgatagt aaacgtcata gcccgccagt
     4261 tggatcacgc gaataccaac gtcctgggcg aactggatag ctttacgcat aatctccagc
     4321 ccctgcgccc gcaccgcgtc atcttcactg cccagcggga aacgacgatg agcagaaagg
     4381 cacatggacg gcacgcgcac gccggtttca acaatcgcat tcaccagcgc cagacgctgc
     4441 tcgcggctcc agtcgaggcg cgacaggcgc tcgtccgttt catctaccga catctcgaca
     4501 aaatcgaagc ccaacgtttt tgccagttgc aggcgttcca gccagcactc cccggcgggg
     4561 agcgcttttt catagatgcc aagcgggatt tgtttggaca acatatccgc tccttagccc
     4621 cacagttcgg cgatggaacg tttgaactga cgtgcggctt ccaccggaga agcggcatca
     4681 cggatactac ggcccgcgat aaagacgtga atcggaatgc ctttgaacag cggcagatct
     4741 tccagcgcca ggccaccagt gacggtgact ttgaagccca tatcggaaag acgtttgatc
     4801 gcggtgatgt ccgcttcgcc ccacgccacg cctgcggcct gcgcgtcacg gctgcggtga
     4861 taaaccacct gctgaatacc tgcgtcgcgc cactgttgcg cctgttccca ggtccagtaa
     4921 ccggtcagtt cgatctgcac gtcgccgtta aactcttttg ccacgtccag cgcgcctttg
     4981 gcggtgttga tatccgcaca gcaaattacc gtcacccagt cagcgttggc ttcgaagcac
     5041 atacgcgaaa ggattttgcc tgcatcggca attttggcgt ctgccagtac gattttgtgc
     5101 gggtagagcg ctttcaggtc acgaaccgca cgcacgcctt cgcccacgca cagaatggtg
     5161 cccacttcga taatgtcgac ttcttcggca atcaggcgag tggtttcgta ggcgctatcc
     5221 atagtctggt tgtccagcgc gacttgcaac atcggtaatg acatgttcaa ttccttctta
     5281 agctgccgcg ttggtgcggt caatgagatc cagtacttcc tgctcggtac ggcaggcgcg
     5341 taaacggtca aaattctctt catcttcaaa caggttgacg atctgcatga tgcccacttc
     5401 ctggtgagta ttggcatcga ccgccgccat ggtgatgagg atatccaccg gatcgttgtc
     5461 atcgtggttg aactccagcg gctttttcag cgttaccaga gagaaaccgg ttttcttaac
     5521 gccctcttcc ggacgcccgt gcggcattgc caggcccgga gcgataacga aataaggacc
     5581 gaactgttca acgccatcca gaatcgcctg gtagtaacgc ggctctacca catctgccgc
     5641 caccagcagg tcaacgccga ttttcaccgc ttcctgccat gtctctgctt cagcctgcag
     5701 acggatggat ttattttcag ccagcgaatc acgtaatttc atggcgcgtc cttacttcac
     5761 gtcctgcggg aaatgctctt tgatcacttc cagcagtttc gggccaaagt cggcaggaga
     5821 gagcatgttg cgcacgccaa ccacgtattt gttgccggtc acggtgattt cgcccgcaat
     5881 gtgcgtagaa gcgatgatga tatccgcgcc actcaactcg cttttgtact cgccaaccgc
     5941 gcagctgttt accgtatggt caatgtttga ttgggttaaa aactggtcca ctttcatctt
     6001 catgatcatg gaactgcctt gtccgttgcc acacacagcc agaatacgta cggtcataat
     6061 caaaactcct tattaagcag actgttctgc cagttgtttt tctgcatctt cttctgcgcg
     6121 cagcgcgcgg ccagcgaaga acatataagc cagtgcaatt acaatgatga cggccataaa
     6181 ggcgataccg atggagaaga agccttgcat catcggcggt gccagaatcg accagtccgc
     6241 catgcccatc caggcactca taccggtgag tttcaccgcc cagacgcaac caaagatttc
     6301 gatcattccc atcaccagac aaatcttcag cgctgcacgc cagccgccga agtggttagc
     6361 gaacacgccg atggtggcgt tggagaagaa catcgggata aagcctagaa taatcagaat
     6421 ggaagagccg caggcgacca ggatgccaac tgcaatcagc tgaccgatgg tgccccacat
     6481 aaagccccag accacggcgt tcggcgcgaa gctatagata gccgcacagt caatcgccag
     6541 aaccgcacct gggatcaggc gctgggagat accgttaaat gcttcagaga gttccgccac
     6601 aaacatgcgc acgccctgag tgatgatgaa gatcgccacc gcgaaggaga aaccggtttg
     6661 caggatgtag accgtccagt gcactttgcc cgccatcgcc tgcacggtgt cgataccgaa
     6721 ggagagcaga atggcgccaa agaagatggt catcacaatc gccgtggaga cgatgttgtc
     6781 gtggaaaatg ttcagccagc ccggcagttt gaggtcttca acgctctcct cttttttgcc
     6841 gaggaacggc gcgactttat aggcaatcca tgatgcaaac tgctgctggt gaccgatgga
     6901 gaaaccacag ccatccgtca cttcctgagt cggcttgtac atcatgttgg aagtgatgcc
     6961 ccagtagagc gaaaccagaa tcgcggtaca gataatggtg gtccacatgg agtagccgaa
     7021 gataaacagc gtaacggcaa tcaacccggc ctgctggaac atgatgtggc cggtcaacat
     7081 gattgtgcga atgccggtaa tgcgacgcaa cagcacgtaa cagatgttca gtgccagcgc
     7141 taacaatacg gcgtaaccca cccagctata ggcatcgccc atgcggtcga tggttgccat
     7201 cattgaagcg taggtatcgg aaattgcgcc gttaatgccg tagacttcgg acattttcgc
     7261 caccaccggt ttgaaggtgc tggtgaggat gccggaccct gcctgcaaca acatgaaacc
     7321 aattatggtt ttaatcgtgc ctttaataat aacgctgaca cttttgcgca gtaggatgta
     7381 gcccagacag gtcacaatac ccagcaacaa cggggcattg gtcatgacct ggttaaaaaa
     7441 tacggtaaag atgttgtaga ggatctccat aacgatctcc tgaagaagag gtaaccgggt
     7501 attccacaca cccggaatgt tgtgcactca ctctaatttt caaaagtaat cacaacaaga
     7561 ttatttatga ttaaatgtga cgcgacccgc aaataattcc acatggatag agcgggattc
     7621 acgcaacgaa acaattattc atttaaattc atttagttaa ttgaattata cattccatta
     7681 aatctttcca tactcaaaat caacaacaac ttccctggca aaaagtcaaa aaatggtcat
     7741 tgccttccag aaaaaatagt gtcaggatta atcaaaacca atcacatatt gattcgaatt
     7801 gaactatcat gaaggtaaat ggcgatgagt aaagtgaaaa gtatcacccg tgaatcctgg
     7861 atcctgagca ctttcccgga gtggggtagc tggttgaatg aagaaattga acaagaacag
     7921 gtcgctcctg gcacatttgc gatgtggtgg cttggctgca ccgggatctg gttgaaatcg
     7981 gaaggtggca ccaacgtttg cgttgatttc tggtgcggca ctggcaaaca aagtcacggt
     8041 aacccgttaa tgaaacaggg tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag
     8101 ccaaacctgc gtaccacccc gtttgttctt gatccgtttg cgattcgcca gatcgacgcg
     8161 gtactggcga ctcacgatca caacgatcat atcgacgtta acgtcgctgc tgccgtgatg
     8221 cagaactgtg ctgatgacgt accgtttatc ggaccgaaaa cctgtgtgga tttgtggatt
     8281 ggctggggcg taccgaaaga gcgttgcatc gtggtcaaac cgggcgatgt agtaaaagtg
     8341 aaagacattg aaattcatgc gcttgatgct ttcgaccgta ctgcactgat caccctgcct
     8401 gccgatcaaa aagcggctgg cgtactgcca gatggcatgg acgatcgcgc ggtgaactac
     8461 ctgttcaaaa cgcctggcgg ctccctgtat cacagcggcg actcccacta ctctaactat
     8521 tatgcgaagc acggtaacga acatcagatc gacgtggcgt taggatcgta cggcgaaaac
     8581 ccgcgcggta tcaccgacaa gatgaccagc gccgatatgc tgcgtatggg tgaagcgctg
     8641 aatgcgaaag tggttatccc gttccaccac gatatctggt caaacttcca ggccgatccg
     8701 caagagatcc gcgtgctgtg ggagatgaaa aaagatcgtc tgaagtatgg cttcaagccg
     8761 tttatctggc aggtgggcgg caaatttacc tggccgctgg ataaagacaa cttcgagtac
     8821 cactatccgc gcggtttcga tgattgcttc actattgaac cggatctgcc gttcaagtca
     8881 ttcctgtaat ctgtcgttta taccggatag cgtggagaag ttcgcgctat ccggcgtagt
     8941 atttcctggc tatttcaaat atcatctaaa aaaatcaaat tttatcggaa tagctcatga
     9001 ctgaagcaca aagacatcaa atcctcctgg aaatgctcgc acaattgggc tttgtgaccg
     9061 ttgagaaagt cgttgagcgt ctgggaattt cgcctgccac tgcgcgacgc gatatcaata
     9121 aacttgacga aagcggcaaa ctgaaaaaag tgcgcaatgg tgcagaagct attacccaac
     9181 agcgcccgcg ctggacgccg atgaatctgc atcaggcgca gaatcacgat gaaaaagtac
     9241 gtatcgctaa agcggcctcg cagctggtta atccgggcga aagcgtagtc atcaactgcg
     9301 gctccaccgc gtttctgctt gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta
     9361 attatctacc gctggcaaat tacctgatcg atcaagaaca tgacagcgtg atcattatgg
     9421 gcggacagta caacaaaagt cagtccatca ctttaagccc gcagggcagc gaaaacagtc
     9481 tctatgccgg gcactggatg tttaccagcg gaaaagggct gaccgcagaa gggttgtata
     9541 aaaccgatat gctgacagca atggcagagc agaagatgct gagcgtggta gggaaactgg
     9601 tggtactggt tgatagcagt aagattggcg aacgcgcggg aatgcttttt agccgtgccg
     9661 atcaaatcga tatgcttatc accggcaaaa atgctaaccc ggaaatcctg caacaactgg
     9721 aagcgcaagg ggtcagcatt ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag
     9781 catctaacgc ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa caggttagca
     9841 gtttatcccg tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg tcggcaggca
     9901 caacgtcatc gtcgaggcca taccacagca gtagaggtct gtcactaagt tgttccaggt
     9961 ggtttgtcgc ttcccactct gccagtggcg cgacaatgtt attgaattca ttctgctgtg
    10021 ctaccgtttc aggtatcagc ggtggaaaca gtgaacgggc gagtgatgta aaatagcccg
    10081 atcccatcat gctggcggta catctcaccg tggggtggcg agcggtaatc cccagtgccg
    10141 tcatcgcgcc catcgacgca ccaccgactg ccagacggtc atcaagcagc cacttttctt
    10201 cggctattgc cgcacgtaaa gtagtgaatt cctgcatact ttgtagcaag atttgccaga
    10261 attgatttaa ccgccgcgct gcgtcaccac taaaacggct accgtgatcg ggcgcatccg
    10321 gcatgatcac ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta tacaccagac
    10381 tggacgaagt aaagccgtga taaaaaatca cgcacggtaa cggggtatct ttttgtccga
    10441 caggataagc atgaagaacg ggaatatctg ccagctcgcg tgattctatt tcaatcatct
    10501 gtctctcctt tttgtctggt atgacatgaa gaaatcctta gcacaagttt ctgctggcgg
    10561 tattagttca taatgttgag atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg
    10621 cgtaccaggt aacaattcgg gaacattccc ccaaagttaa attaataagg cactacacta
    10681 tggttagcag gaaacgtaat agcgttattt accggtttgc cagtttatta ttggtgttga
    10741 tgttaagtgc ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca gtaacggacc
    10801 atccgcaaga gattcgccgc gaccagacgc aagggttaca gcgaataggt agcgtaagca
    10861 caatggttcg gggttctccg gatgacgcat tagcagaaat cagagcaaaa gctgtcgctg
    10921 caaaagctga ttattacgtt gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt
    10981 attcacaagc cattttgtac cgtaaataaa tcagacaact tacatagtca gctttacatt
    11041 gctttgcgtc cctatgcgtt tacctgtgca caataaatta ccggctggaa gcctgggcgt
    11101 ttattaccgc gatggaatgc cctgcaacat gtggggaaac gaaaaatgga gctgacgatg
    11161 aaacaattac ttgcctcacc ctcgctgcaa ttagtgactt atcctgcgag cgccacggca
    11221 cagtctgcag aattcgccag tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc
    11281 tcagttagca atatttcagg ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc
    11341 gatgaacaag gtgcgtcatg gtaccgcatt attaccatgt acgaagacca gcagcctgat
    11401 aactggcgcg tacaggcgat actgtatgca tgagtgtcat ctacagcaaa tgaacaatgc
    11461 cggatgcggc gcgagcgcct tgtccggcct acaattttca catatttcat ttagttaatc
    11521 gaaaccagcg tcgcatcagt cgtatacgct tacacacaca ctccccccgt cgcccgcagc
    11581 aataaatcat tctggatctg ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc
    11641 tgacaccacg cctgcgccat tggcggagaa gcatatttca acatttgcgc accgcagccc
    11701 agcaggaata gctgatgagt aatctctcgc cccagttctt cagctggctt acgcagctgc
    11761 tgctgtaaac gacgaaccgc gcaatcaaaa tagcgatcct gccctttcac ttctacaaat
    11821 gcttccgaca ataagtcgta tacgcccgct tgcttattga gaacgcgcag cacatccagg
    11881 cacataatat tgccggaacc ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc
    11941 ggcagctcgc tctcctcgca ataaccaatg ccgcccagca cctccatcgc ttcggccaca
    12001 aacggcatac ctcgtttgca gatcacaaat tttgccgcag gcgtaaataa acgcgcccac
    12061 agagcttctt tggcatcggc gcgccggtcc cacgctcgcg caagacgaaa cagcaacgcc
    12121 gtttgccctt caagctgaag tgccatgcga cttaagacat gacgcataag gggctgttgg
    12181 atcaatggat taccaaaaac atggcgttga tgcgcatgat aaatcgccag cgagaacgca
    12241 cggcgcatca tggcatggct acccagggcg caatcaaaac gcgtcatccc ccccattttc
    12301 aggatcagac gaattccttc cccttcctgc cccaataacc agccaatggc atcctgaaac
    12361 tccacttcac aactggcgtt agagcgatta cccagcttat ctttcagccg ctcgaggcga
    12421 atcgcgttgc gttgcccgtc aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc
    12481 gcggtctgcg ccagcaccag atgcgcatcg ctttgcggaa ctgagaaaaa ccatttatgc
    12541 cccaccagcc gataagagcc atcctccaga cgctctgcgc gggtggtgtt gctcatgaca
    12601 tcggaaccgc cctgcttttc cgtcattccc atgccaatca acaaaccgcg tttttgccca
    12661 cctggcaata agtgagaatc gtagcgatcg ctcaacagcg gtgtggtcca gtcctgaaac
    12721 ggcgcgggta acatctgtaa caacaatggc gtggcggcaa aggtcatggt tatcggacat
    12781 aacgaccccg cctcaacctg tgcgtgcaac ataaaacgcg ccgcgcgcgc cacaaatgcg
    12841 ccggagcgag cgtcttcttc ccaggcaaga ttgtgcaccc gattggtaca tagtgcctgc
    12901 atcagcaggt gccaggcggg gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg
    12961 tagcgcaaca gttcaggcgg attcacattc gccagccgcc ccagttcaag ggattcagcc
    13021 gttcctaact gctgaccaat actggctaga aaatcgctat cccagccagc accttcacgc
    13081 gttaccgctt cgcaaagcgc gccatcagac aggtataagt tgctgttatt taatggtata
    13141 ggttgattaa aaacggtgtg agtttgccag tgcactgtgt ctccctccat caatggcagt
    13201 caccattagt atggtcactg ccatggattc atgacagaaa gagattgcta aaacattctt
    13261 atccagcaat gtaatgcgga acgaaacgtg aggtgtcttt ggtgatcagt gtgttatctt
    13321 cacggatccc cattccgcac gcttcatcat cgacaatcca gctaccgatg agtgtgtagc
    13381 tatcgccaaa ccgcggcaga ggttgaaacg cctggtagat catcggttca tcggcgtaat
    13441 caccatcagc gtggtcaacg acgttattct ggccgtcgaa aatggtgaca tttccgcctt
    13501 cgcgcgagta gatcggttta cgcacatagc tttcgccagc ggcaatctgc ggtttttcac
    13561 cctcaaacca ggatgcaaga aggttaggat gaccagggaa gaagcgccaa agcagcggca
    13621 ttagcccttt attactcaag atacttttcc ataacggctc cacccattgc tcgcgacgct
    13681 tgcgcagcag cggaccgtta tcgtcacgca tcatccactc cagcggatac agcttaaatg
    13741 cacgctggat gacattatca tcaagatcgg tcagtacgcc gccgacgccc aaaccgagat
    13801 cttcaatgta gataaaccgc gactcctgcc ctgcctgctg ggcgcagtcc tgcaagtaca
    13861 gcacggtact cctgtcttca tcggtgtcct gacagcagca aaaataaaac ggttcccgac
    13921 tgtaaagctc gctaaagcgt gaaatcaggc gttcctgaat agcattgtac tgatcggcat
    13981 cacgcggaat aatgccgctg cgccgggcat cctccagcca cagccactgg aaataagccg
    14041 actcgtacaa tgaagttggc gtatcggcgt tgtactccag cagcttcacc ggcgcattgc
    14101 cacaccaggc aaaatccatc cgtccataca gcgaaggatc gcgggcgcgc cagctttcag
    14161 cgatcacatc ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcatctttta
    14221 ccgcgcgatc caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct
    14281 gctcttcaat ctggcgcaga gtaaaacggt aagcccgact ctcatcccaa tagatttcat
    14341 tgtcgatgat atgaaagtcg aaaccgttgt cagcggcgat ctggtccaga tcccgtcgca
    14401 caggaacgtt gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg
    14461 accatagccg ccgcgagaaa cggttgacgc ttttttggtg gtaaagccgc ctgaagagta
    14521 agactctttt ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgcgccagac
    14581 ggggcgggaa gcaaatgagg atccgccgct gttatagaca aacggctcat cgcgatcttt
    14641 acgaataacc cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt
    14701 gtcgtagtag caattttcgt acttagactg acagttctgc tgagtcatat tcttcggaac
    14761 atcggcataa aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt
    14821 accgtcatca atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac
    14881 atcgccacta tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa
    14941 cgttaagtat ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc
    15001 aatgcgactg atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc
    15061 catgatcgtc cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc
    15121 ggttcccata aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg
    15181 catataaagt ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca
    15241 ggcgaaatag tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta
    15301 acccaacaat gtaccgctga acgccagtga cgctgcggta ttattgtttt tgattaattg
    15361 ccattcattg tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac
    15421 gccaataaaa aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt
    15481 ccttgtaatt aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc
    15541 gaaaataacc actctggctc gccgagatcg ttaaaagatt cctcaccatt tagcagcaaa
    15601 tattcgtaag tatcttcggt cacttgccgc tggtagccca tggtaaaatt atggacttcc
    15661 catttagcat gattttgatt ttctactttt tccagcatgt agacgggttc gatatttccc
    15721 ggttcttcgc tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc
    15781 cccatcgttt tggcgttgat cgcctcgcgc cagtgattct ctttactgat accgtagctc
    15841 tcttcataga caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta
    15901 ttgatttgca gaaactcatc gcctgaagtg tagtagcgga aaatctgact accgccgccc
    15961 agatcgatgt ggctgacagc gactaccgta aattcctcac ccggcagcgc aatcagcaat
    16021 tcatcttcca gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt
    16081 cccagcggac cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa
    16141 ccagacatat tttatcctta aaatagttgc cagccttgca gtgaggctgg caaaaaaatt
    16201 actcgccctg ttggcgttgc agtcttgcta atacatcctg ggcgctactt ttattgctac
    16261 cgccaattcc ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttttcta
    16321 actgtgcggc agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta
    16381 aggattctgc tgctgtcgaa acgctggaag atgcgccaac ggtagaagtc gttacagcct
    16441 gttgtgcacg ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag
    16501 cgatacgctg cgctgtcgct gcaactgctt tttccacgcc atcacgggag acttccagat
    16561 tcgacaacac ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa
    16621 cttcgttaat caacgacgga ttaacgttct tgcttaacgc ttccagcgta cgggcttcca
    16681 gactggcttt gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca
    16741 gcagatcaac gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct
    16801 gttccagcat gcgcacaccc tgggtttctt caatggattc ttccgcctgg gagataaacg
    16861 atttccccag cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt
    16921 gtgaatattc ttctgtaatt tcagccagat ccaatgcgtt atctaccagc gaggttatct
    16981 caagcaggat atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact
    17041 cttcttgttg tacgctggag attcctaccg atgacaacgg catcatcttc tgatttctta
    17101 ataagaaggt attaaattca tctggattgc tgatactact caccggacaa ataaaggttt
    17161 cgataatcat ttggcgggaa gtaaaaagaa tattgatttg caaatcgcca taatcattca
    17221 ttttaataat taatgcgttc tcgctatttg ttacatcaat attttgttca ggtacagttt
    17281 gcagcgccgt cgccagcgcc
//
LOCUS       DAZFTN010000095        17097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFTN010000095 DAZFTN010000000
VERSION     DAZFTN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..1229
                     /gene="arnB"
                     /locus_tag="ACHKV2_003638"
     CDS             90..1229
                     /gene="arnB"
                     /locus_tag="ACHKV2_003638"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO6941450.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1233..2201
                     /gene="arnC"
                     /locus_tag="ACHKV2_003639"
     CDS             1233..2201
                     /gene="arnC"
                     /locus_tag="ACHKV2_003639"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO6941451.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2201..4183
                     /gene="arnA"
                     /locus_tag="ACHKV2_003640"
     CDS             2201..4183
                     /gene="arnA"
                     /locus_tag="ACHKV2_003640"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO6941452.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4180..5070
                     /gene="arnD"
                     /locus_tag="ACHKV2_003641"
     CDS             4180..5070
                     /gene="arnD"
                     /locus_tag="ACHKV2_003641"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO6941453.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5070..6722
                     /gene="arnT"
                     /locus_tag="ACHKV2_003642"
     CDS             5070..6722
                     /gene="arnT"
                     /locus_tag="ACHKV2_003642"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO6941454.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6719..7054
                     /gene="arnE"
                     /locus_tag="ACHKV2_003643"
     CDS             6719..7054
                     /gene="arnE"
                     /locus_tag="ACHKV2_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO6941455.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7054..7440
                     /gene="arnF"
                     /locus_tag="ACHKV2_003644"
     CDS             7054..7440
                     /gene="arnF"
                     /locus_tag="ACHKV2_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO6941456.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7434..7700)
                     /gene="pmrD"
                     /locus_tag="ACHKV2_003645"
     CDS             complement(7434..7700)
                     /gene="pmrD"
                     /locus_tag="ACHKV2_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO6941457.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7810..9165)
                     /gene="menE"
                     /locus_tag="ACHKV2_003646"
     CDS             complement(7810..9165)
                     /gene="menE"
                     /locus_tag="ACHKV2_003646"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO6941458.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9162..10124)
                     /gene="menC"
                     /locus_tag="ACHKV2_003647"
     CDS             complement(9162..10124)
                     /gene="menC"
                     /locus_tag="ACHKV2_003647"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO6941459.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10124..10981)
                     /gene="menB"
                     /locus_tag="ACHKV2_003648"
     CDS             complement(10124..10981)
                     /gene="menB"
                     /locus_tag="ACHKV2_003648"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO6941460.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10996..11754)
                     /gene="menH"
                     /locus_tag="ACHKV2_003649"
     CDS             complement(10996..11754)
                     /gene="menH"
                     /locus_tag="ACHKV2_003649"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO6941461.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11751..13421)
                     /gene="menD"
                     /locus_tag="ACHKV2_003650"
     CDS             complement(11751..13421)
                     /gene="menD"
                     /locus_tag="ACHKV2_003650"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO6941462.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13510..14805)
                     /gene="menF"
                     /locus_tag="ACHKV2_003651"
     CDS             complement(13510..14805)
                     /gene="menF"
                     /locus_tag="ACHKV2_003651"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO6941463.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14884..15189)
                     /gene="elaB"
                     /locus_tag="ACHKV2_003652"
     CDS             complement(14884..15189)
                     /gene="elaB"
                     /locus_tag="ACHKV2_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO6941464.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15244..15705)
                     /locus_tag="ACHKV2_003653"
     CDS             complement(15244..15705)
                     /locus_tag="ACHKV2_003653"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6941465.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15770..16687
                     /gene="rbn"
                     /locus_tag="ACHKV2_003654"
     CDS             15770..16687
                     /gene="rbn"
                     /locus_tag="ACHKV2_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO6941466.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16875..>17097
                     /locus_tag="ACHKV2_003655"
     CDS             16875..>17097
                     /locus_tag="ACHKV2_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697257.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6941467.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRN"
BASE COUNT         3993 a         4618 c         4357 g         4129 t
ORIGIN      
        1 ttaaaatttc gctctaaatg gcaaaatata cacaacactc tttatagcaa atataagtgg
       61 acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt tcgagaccag
      121 caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt tggatcacaa
      181 ccggtccgaa gaatcacgcg cttgaacaag ccttttgcca gttgacggga aatcagcatg
      241 ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg ttggaaattg
      301 gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc aatatgattt
      361 ccttgcaggg tgcaacgccg gtaatggtgg atgtcgatcg cgatacgctg atggtcacgc
      421 ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg gtgcattatg
      481 ccggtgcgcc agcagatatt aacgccattc gcgccattgg cgaacgttac ggcatcgcag
      541 ttatcgaaga tgctgcccat gccgtaggta cgtattacaa agggcgacat attggcgcaa
      601 aaggtaccgc tattttttca tttcatgcca ttaaaaatat tacctgtgcg gaaggaggcc
      661 tgattgttac tgataatgaa aatattgccc gccagctacg gatgctgaaa tttcacggcc
      721 tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct gaagtcttaa
      781 cgccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg acacagttag
      841 ccaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat cagcaagcac
      901 tggcagctct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc
      961 atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat gcgttgatgg
     1021 aagcgttaaa agaaagaggt attggtaccg ggttacattt ccgcgccgct cacacacaaa
     1081 aatattatcg cgagcgtttt ccctcgctgt cgttaccgaa taccgaatgg aatagcgaac
     1141 gcatctgttc tttgccgctg ttcccggata tgactaccgc cgatgccgac cgtgtcatca
     1201 cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct gttaagaaag
     1261 tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta atcaggcgca
     1321 ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt gatgacggca
     1381 gtagcgataa ttccgcgcat atactggtcg aagcctcaca agcggagaac agccatattg
     1441 tatctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg ggattcagtc
     1501 acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg ccagaagaaa
     1561 tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact gtacgccaga
     1621 accgccagga cagctggttt cgtaaaacag cttcgaagat gattaaccgg cttattcagc
     1681 gcaccaccgg caaagcgatg ggtgactacg gttgtatgct gcgcgcctat cgccgtcata
     1741 ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt ctggcgaata
     1801 tcttcgcccg ccgtgccatt gaaattccag tgcatcatgc cgagcgtgag tatggtgaat
     1861 ccaaatacag ctttatgcgc ctgattaatt tgatgtacga cctggtgacc tgccttacca
     1921 caacgccgct acgtatgctg agtctgctcg gcagcattat tgcgattgga ggttttagca
     1981 ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg gcagaaggcg
     2041 tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc ggcatgggat
     2101 tactcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc cgctattttg
     2161 ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc
     2221 ctaccacgat atgggatgcc tcggtattga agccctgctg gctgccggtt acgaaattag
     2281 cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt cggtggctcg
     2341 tctggcggcg gaaagaggca ttccggttta tgcgccggat aacgttaatc atccgctgtg
     2401 ggtggaacgc attgcccaac tctcaccaga ggtgattttc tctttttatt atcgccatct
     2461 tatttgcgac gagattttgc agctcgctcc cgcaggtgca tttaatctgc acggttcgct
     2521 gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg gtgaaacaga
     2581 aactggcgtt acattgcacc gaatggtaaa acgtgccgat gccggggcca ttgtggcgca
     2641 actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata aattgtgcca
     2701 tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta atattctgga
     2761 aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg atgacagttt
     2821 cctcgaatgg cataagccgg catccgtact gcacaacatg gtacgtgccg ttgccgatcc
     2881 gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt
     2941 tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc cactgctgat
     3001 tgcctgtggc gatggcgcgc tggaaatcgt caccggacaa gcgggcgacg gcattactat
     3061 gcagggctcg caattagcgc agacgctggg cctggtgcaa ggttcacgct tgaatagcca
     3121 gcctgcctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga atggctttat
     3181 tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt acggtctgga
     3241 tattggcagc gatgcgataa gccgttttct gaatcatccg cattttcact ttgtcgaagg
     3301 cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg atgtcgtctt
     3361 gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc gcgtatttga
     3421 actcgatttt gaagagaatc tgcgcattat ccgctactgc gtgaagtacc gtaagcgaat
     3481 catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact tcgatgagga
     3541 ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt cggtatcaaa
     3601 acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac agttcaccct
     3661 cttccgcccg tttaactgga tgggaccacg actggataac cttaatgcgg cacgaatcgg
     3721 cagctcccgc gcgattacgc aactcattct caatctggta gaaggttcac cgattaagct
     3781 gattgatggc ggaaaacaaa aacgctgctt tactgatatt cgcgatggta tcgaggcgtt
     3841 ataccgcatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca acattggcaa
     3901 tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga gcttcgaaaa
     3961 acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg aaagtagcag
     4021 ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc gcaatgccca
     4081 ccactgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg aaacgctgga
     4141 tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt aggcttacgc
     4201 attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg
     4261 agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa tatgggccgc
     4321 catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc aaacgcggca
     4381 tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa agagattggt
     4441 catgccaatg ccgatatcat tcgtgaagcg gctaaatatc acgaagtcgg cctgcacgcc
     4501 tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac aatgatcgac
     4561 gatattgcac gcggccttcg cactctggaa gagattatcg gtcaaccggt aacctgttct
     4621 gccgctgcgg gctggcgtgc cgaccagcag gtgatcgaag caaaagaagc gttccatttg
     4681 cgctacaaca gcgattgtcg tggggccatg ccgttccgtc cactgctcga atcaggaaac
     4741 cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat tggtcgggat
     4801 gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga taaaggcacg
     4861 ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa ttttgtggat
     4921 ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga actgttatca
     4981 gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga aggctggctg
     5041 ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt atcggcctct
     5101 tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgcta tggcaacccg
     5161 atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac tggattgtgc
     5221 cccatctgtt agggctacgt tattttgaaa aacccattgc cggatactgg attaacagca
     5281 ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga
     5341 ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc gataaacgtc
     5401 tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc atcggcactt
     5461 atgccgtgct cgatccgttt attgccttct ggctggtggc gggaatgtgc agcttctggc
     5521 tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg ctgggaatca
     5581 cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg gtattaagcg
     5641 tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac ggctggctgg
     5701 cggtcatcag ttgcgtactg acggttctcc cctggggact gacgatagcg cagcgggagc
     5761 ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca ctggatgatg
     5821 cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt agtctgccgt
     5881 ggctgggatt actccccggt gcactataca caggctggaa aaaccgcaag cattccgcaa
     5941 ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtt gctaagggta
     6001 aattgcccac ctatattctt tcctgctttg cacctttggc aatgctgatg gcgcattacg
     6061 ctttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg attaacatcg
     6121 cttttggcgt cactggcatt attgccacat ttgtggtctc cccctgggga ccaatgaaca
     6181 cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg attttttcgc
     6241 tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg tcttttgccg
     6301 cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac agagtcatgg
     6361 aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag ccaagccgct
     6421 atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagtctg caacgcgatg
     6481 acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat ccggatgcga
     6541 aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat cgtcaggagg
     6601 ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt ctcgccattc
     6661 cgcccgcaga tgccatcgat cgtcaggagc gtctggtgct gattcagtat cgtcccaaat
     6721 gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa
     6781 acaggcaacc tgctttgtgg cgataagcaa acggcgcaaa catatcgtgc tgtggctggg
     6841 actggcgctg gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct tgcagaacgt
     6901 accggtaggt attgcctacc cgatgttaag tctgaatttt gtctgggtga cgctggctgc
     6961 agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg cgttcattat
     7021 tggtggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatatgggg attattcagc
     7081 gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcgagtca tctgccgccg
     7141 atgacgcact tatgggattt tatcgcggcg ctgttagctt ttgggctgga tgcccggata
     7201 ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa aacactacat
     7261 aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct ggtatggatt
     7321 gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact actgggagta
     7381 gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca acgttactga
     7441 gttttccctg ccactttaca ctgccgttcc cactcatcgg ggctataaca gcttgcagag
     7501 aggactttca ccgtatgcag cttttcacga ttaatacaat aaagcgcatt ctgcaaaggc
     7561 gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat catttttatc
     7621 gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt gcagcacgat
     7681 tttttgacca gccattccat tgtattatcc tgtttgctaa gagttttcat atcaatttac
     7741 agattaacag ataaactctt tatcaacggc aatggaatgg cagaactgag aagtgaaacg
     7801 gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg aaattttaat
     7861 accgccgttt ttcagctccg gcggcaaagt cagccaacgt accggttgct gaaaacgggc
     7921 cagcttatct ttcacccatt cactaagatc aacgctctcg tggtcatact ccatcaccgc
     7981 caccggtcga tgaccaaact ccttgtcggc aacggggacg ataaacacct gcaaaaccgc
     8041 aggatgtgca gcaattacgc gctcgacttc ttccgtctga ataccctctc cgccactgaa
     8101 gaatagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct caccgcgatc
     8161 gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt tacgccagta
     8221 accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt tcacttcccg
     8281 acccggcagc ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac acaccgtgga
     8341 ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc gcgcctgttc
     8401 cgtcaactcg accgggatag ccgcgccgcc aagtaacacc gctttcaggg aaacggaact
     8461 gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag tacagcctgc
     8521 cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag cgtataacca
     8581 gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca accagtcatc
     8641 gtgatcgcca aacggaatca gcgataacac cccttcggca ctggcaagat gggcctgata
     8701 agtatggaca gcggctttcg gcaacccggt agaacccgag gtcaacgtca ttgagcacag
     8761 acgcgtcggc tgccacgtag cgacatgtgc gccttcaacc cgctgaatgt gcagcgacgt
     8821 taatgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg tcagattggg
     8881 cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca cccgcgcccc
     8941 gcattgcagt aacgccagcc aagcgagcag cgtttgcggc gtattccacg cccgcaacat
     9001 cacgccgctg tcctcaacca caccctgcac cgcaaatccg gaggctaatt catcgacgcg
     9061 agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga tggcttctcc
     9121 ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa ccgctccagt
     9181 gcatcaactt ccacgacagg cagcgtgcta cccggccagc gacgtacctg ctgcgcctgc
     9241 attagatcca gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc agcaatccgc
     9301 gccagttgcg ttaagcctaa gctcgattca atggaagaac tgatcactgc cgtcagcccc
     9361 agcgcgtgcg ccgcctgtac ctgctcgcga actttctcca gactgcccgt gagcgtgggt
     9421 ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg ctcgcgcaag
     9481 ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga atcatcgcgg
     9541 gttttgcacg gctcttcgag aaacgcgatg cggtggcgat aatccgggtt aacgtatttg
     9601 gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg caaatgcaga
     9661 tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc ttcgtacaat
     9721 cccactttga ccttcgccac tttctcgcct ggcatatctg caagtttgag gatcagatcg
     9781 tccggatcgc cattacacag tggtgccgta cggtaattgg ccgcctgcgg gagcgtatct
     9841 gccagctccg ccaatgcaca acttacgcca aaggcaacgg aaggcatctg cggtaattcg
     9901 caatcgcctg ccagccagtt atttacccag gcaagcagca cactttgcgc ctcttcccag
     9961 gtttcctgac tgaagcccgg cagtggggag atctcccccc acccttcgcg ctcgccctca
    10021 cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc
    10081 gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga ttccgtttga
    10141 atttgctgaa gtcaggctga cgtttctggt tgaaggcgtt gcgaccttcc tgaccttctt
    10201 ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagacct gcctgaccgt
    10261 cacagtcggc gttcagtgca gctttcaggc agcgcagcgc catcggactg ttttgcagca
    10321 tttcacggca ccagcgaacg gtttcttttt ccagatccgc cagcggtacc acggtgttga
    10381 caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac cagatttcac
    10441 gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg ccgtcgaagg
    10501 aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgccgcgata gtcaggtcgc
    10561 acatcatgtg cagaacgtga ccaccgccga tggaatagcc agccaccatc gcgacaaccg
    10621 gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga tgtacgccgg
    10681 aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg gagcagaacg
    10741 ctttatcgcc tgcgccagtc agaatgatca cgccgacgtt atcgtcataa cgcgcatctg
    10801 ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc acctgcggac
    10861 gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg tcctcaaagc
    10921 cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca ggataaatca
    10981 tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta cgccagcggg
    11041 attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg cagccagttc
    11101 cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa acgcaaatgt
    11161 gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg cctccagcat
    11221 ggcggcaagg gttgcgccat tattgttgct gcgcagcacc accagctccc ggcgttgatc
    11281 gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg cggttaatgg
    11341 ttcggaacga aaacgctgcg cccattggcg atcggaacgc tgacgttccg cgcgttgttc
    11401 agcattttgc agccccggat gaccgccttc gacaacaacc ccacaaagcc ccgccagccc
    11461 ctggcaagcg gccatcatcg ccacccgtcc gccaagcgag taccccacca gccagaagct
    11521 aaggatgttg taactaacca gggttttacg cagtaagtcg gtgacatcat caaatccatc
    11581 gacgctaatc gccgccgaac caccgtgacc tgggagatca acatacaacc gtgagtagtc
    11641 ggcaaacgcc tcgccgactt cctgccattc gaggcaatcg ccggaaaaac cgtgcaaaaa
    11701 caccagccag ggtaaacctg gtttgccgtg ttttgcctgc gcgtgcagga tcataaatgg
    11761 cttacctgcg ccagcagctg ctggagcgtt tgcgcgccgt cggtgtcgtt aaccaccatt
    11821 tcaattaccg tggtggtcgg agtacgccag gcgtcggcaa gtaccgtttc aagttcctgc
    11881 cagttttgcg gacgatgata tttcagctca aacatcgcgg cagcgtgctc aaaatggacg
    11941 ttttgcggca tcagatagaa acgctcgcgc tcgcttttcg gcgttggcag cagcgagaaa
    12001 atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcggaaac ctggcgcaat
    12061 aacgccagcg cattcaggtc gtaaagtgcg gagagatcgc ccacaatcgc cagcgtcggt
    12121 ttgccgcttg cccgctgaac gccggcggca gtcgagagca gcccgtcgat accgctggcc
    12181 ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt
    12241 accaccaggc tgttaccgac aaacaattgc ccctgttcag gcagatagtc gctgatgcga
    12301 tgcgccagtt gcgcttcgcc aaacgcatcg cgacaggcaa taaccgcctg cattgcctgt
    12361 tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg atgcagttcc
    12421 agccagtcgg caatattggc aattaagcgg cgtccacgat ggtgtgccgg atcaagccgc
    12481 ccttcgatgt catccacaat ccagtactct tcgggttcac agcttgcctg ccattgcagg
    12541 agccgtttgc ccgtcaggct gcttcccagt tgcaccacaa tttgcgcctg ttgcagttcg
    12601 ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg tccggtttgc
    12661 gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagggc aacttttttg
    12721 ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgccg ccagaagaac
    12781 cagtcgcgct gtttttcgct ctcaagacga ggcgcttcac gcagccacgg cttatcatcc
    12841 tgccaccagt cacccagacg ctgttgccag ctaagcccag tgtcgtccat ctcgccatac
    12901 agcggttcag caaacgggca gttgatatgg accccaccag catgaagcgt accgagagca
    12961 tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg cggcagtgaa
    13021 atactgtgcg tggggtgaga ggcgaacatt cccggctgac gaatcgcctg attcgcgccg
    13081 cagtcaatta gctccggcgg gcgatcggcg gttaacagga tcagtttttc tccggttaac
    13141 ccagcttcaa tcagtgccgg atagagattt gctaccgccg tgccggaggt cacaatcacc
    13201 gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccag tccacgctca
    13261 tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt taacggtgta
    13321 gaacgcgagc caggggcgat gcagatgtgt cttacgccgt gacgcgttaa tgcttccaga
    13381 atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc cggtatcaat
    13441 attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg gtaatgatgc
    13501 gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt tgtcgatttc
    13561 ctgccactct tgctcggggt cggaaccacg gacaatgccc gcgccagcat atagccgcac
    13621 gacattgccg ctgatttttg ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga
    13681 gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgat gggcgataaa
    13741 ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca actgatgtaa
    13801 acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa gatgctgcac
    13861 tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat cggcctgtaa
    13921 tcgttggcag atatcttcca ccaccagcat gttctcgcgc tggtttttat catccgccat
    13981 cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta ctgttcccgc
    14041 cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc
    14101 aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattga gattcagtcg
    14161 acgactggca gccatcatcg ccgccgcatt gaccggactt gcgaaatgca ggtcagttgc
    14221 ccgagcaagc accactttgt cgagctcacc ttcggcgatg gttttcgttg ccagttcgat
    14281 taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtagtgg ttaaatgtaa
    14341 cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tctcttgcct taatcgcatc
    14401 gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac cgccacagcg
    14461 tcgccattcc aggcgtggta aaagtaaatt gccctgcgac gggtcaaacg cattcagccc
    14521 ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa
    14581 cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat tacgttgttg
    14641 ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat caaaagcgtc
    14701 tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa tctcttgcga
    14761 caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga cctctccctg
    14821 ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg gtgtagggag
    14881 gttttaacgg cgtgccagca acagtcctag taccagccca acggccgcac ccacaccaat
    14941 tccctgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct gcttcgcccg
    15001 atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca gcgctttttc
    15061 tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg agcggagcac
    15121 ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg
    15181 attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga caagggttat
    15241 tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat cttcttcata
    15301 gatctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc
    15361 cccgaggtag acaggcttat caggccagtg atgcgtgcaa gtgtcgaggg ttttactcat
    15421 cagttgctgc cccacttttt cgccacgcaa cgcttcactg acaatcaccc gaccaataac
    15481 gaccggctca agatcatcat cacttttcag aatcctcgca tatgccacca gttcatcatt
    15541 tttccaaccg agaatatggc gattatcccc cgtcaaatca tcaccatcga tatcctgata
    15601 agggcaattt tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg cataaagctg
    15661 ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc tctcctttta
    15721 gatgtatgcc ttatactagc cggaatattt tttgaaacgt gatgaactca tggaattaat
    15781 ttttttaggt acttcggccg gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct
    15841 aaacctgcaa catccgaccc agagcggact ttggttgttt gactgcggtg aaggcaccca
    15901 gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt ttatcagtca
    15961 ccttcatggc gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg
    16021 cattatccaa cccttaacga tttatggtcc gcaaggtatc cgtgaatttg tggagaccgc
    16081 gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg
    16141 cgaaattctc gatgatggcc tgcgcaaagt gaccgcttat ccaatggaac acccactgga
    16201 atgctatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg cccaggcatt
    16261 aaaagctgct ggcgtaccgc ctagcccact gtttcaggaa ttaaaagcgg gcaaaacaat
    16321 catgctggaa gatggaaggc agattaacgg cgcagattac ttagctgctc cagtgccagg
    16381 taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcacttg acctggctaa
    16441 aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag ccaaagccaa
    16501 tagtcgcggt catagctcta cacgccaggc tgcgacactg gcccgtgagg ctggagtcgg
    16561 taagctaatc attacccacg tcagctcgcg ctatgatgac aaaggttgtc agcacctgtt
    16621 acgtgaatgc aggtcaattt tcccggcgac tgaactagcg aatgatttca ccgtgtttaa
    16681 cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc gcaacccgcg
    16741 ctacatatat ccactcacac gggcatattg agcgattttt gatatttatt catctcctta
    16801 cgcactatat tcagagcaat aagttgcctc tttttatctt cattcagcac aacctttgca
    16861 actaaaagga gcatatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaaccc
    16921 aactcagtca gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa
    16981 aaaaaacagc gttatccgga gatgaagaaa gcattgagtt actgcataat attgcgttag
    17041 gttatgatga atttgggaaa aaagctgaag atattcttta ccatattgtt agaaacc
//
LOCUS       DAZFTN010000096        16932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFTN010000096 DAZFTN010000000
VERSION     DAZFTN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..5578
                     /locus_tag="ACHKV2_003656"
                     /pseudo
     CDS             150..5578
                     /locus_tag="ACHKV2_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5694..6758
                     /gene="hipA"
                     /locus_tag="ACHKV2_003657"
                     /pseudo
     CDS             5694..6758
                     /gene="hipA"
                     /locus_tag="ACHKV2_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            7029..7298
                     /locus_tag="ACHKV2_003658"
                     /pseudo
     CDS             7029..7298
                     /locus_tag="ACHKV2_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7732..8295
                     /gene="fimA"
                     /locus_tag="ACHKV2_003659"
     CDS             7732..8295
                     /gene="fimA"
                     /locus_tag="ACHKV2_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6941468.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGASFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            8656..9366
                     /locus_tag="ACHKV2_003660"
     CDS             8656..9366
                     /locus_tag="ACHKV2_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6941469.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9408..12059
                     /locus_tag="ACHKV2_003661"
     CDS             9408..12059
                     /locus_tag="ACHKV2_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6941470.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            12073..12603
                     /gene="ydeS"
                     /locus_tag="ACHKV2_003662"
     CDS             12073..12603
                     /gene="ydeS"
                     /locus_tag="ACHKV2_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6941471.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12616..13119
                     /gene="ydeR"
                     /locus_tag="ACHKV2_003663"
     CDS             12616..13119
                     /gene="ydeR"
                     /locus_tag="ACHKV2_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6941472.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            13179..14093
                     /gene="ydeQ"
                     /locus_tag="ACHKV2_003664"
     CDS             13179..14093
                     /gene="ydeQ"
                     /locus_tag="ACHKV2_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6941473.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            14427..16706
                     /gene="ydeP"
                     /locus_tag="ACHKV2_003665"
     CDS             14427..16706
                     /gene="ydeP"
                     /locus_tag="ACHKV2_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO6941474.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT         4669 a         3661 c         4221 g         4381 t
ORIGIN      
        1 tcgaaagtat tagcaagtta gccaaattga cactttccca cctccaacta acactcaata
       61 cagacctctg acaatttaat aaatttcatt tttaatgtct gacgactcat ctctttcgtg
      121 gctgtattgt ttgtatttgg agcgtttata tgaatagaat ctatcgcgtg atatggaatt
      181 gcactctaca ggtatttcag gcctgctcgg aattaactcg cagggtaggt aaaacatcaa
      241 cggttaattt gcgaaagtcc tctggactga caacgaaatt cagtagattg acgctgggtg
      301 ttttgctggc actaagcggt tcagtgtctg gtgcgagtct ggaagttgat aatggtcaga
      361 ttaccaatat tgatactgat gttgcttatg atgcctacct ggttggctgg tatggcactg
      421 gagtgcttaa tattttggct ggcggtaatg cctccttaac cactattact accagcgtca
      481 ttggcggtaa tgaggattcg gagggtaccg ttaatgtttt gggtggcacc tggcgattgt
      541 atgatagcgg aaataatgca aggcctttaa atgtgggtca atccggaacg gggacgctga
      601 atattaaaca gaagggtcac gtcgatggag gctatttaag attaggtact caagctgcag
      661 gcgtcgggac ggttaatgtt gagggagagg actcggtttt gacgaccgaa ttattcgaaa
      721 tagggagtta cggtacgggc tcattaaata ttacggataa gggttacgtc acgagttcaa
      781 tagtcgccat tttaggatat caagcgaaca gtaatggtaa ggttgtcgtt gaaaagggtg
      841 gcgagtggct aataaaaaat aatgattcct caattgaatt tcaaattggt aatcaaggaa
      901 ctggggaggc gactattcgc gagggtgggc tgattacggc tgaaaatacg attatcggtg
      961 gcaatgccac cggtgtcgga accctgaatg tgcaggatca agactctgtc atcacggtac
     1021 gcagactcta taatggatat ttcggtaatg gcgcagtcaa tatttccaat aatggactaa
     1081 ttaataacaa agaatattca ttggtgggcg ttcaggacgg ttcccacggt gtcgtcaacg
     1141 tgaccgataa agggcattgg aatttcctcg gaacgggcga agctttccgc tatatctata
     1201 tcggtgatgc tggcgacggt gaacttaatg tctcgcgtga aggtaaagta gattcgggaa
     1261 ttatcactgc ggggatgaaa gaaacaggca caggcaacct tactgttaag gataagaact
     1321 ccgttatcac taatctcgga actaatcttg gttatgacgg ccacggcgaa atgaatatca
     1381 gtaatgaggg gcttgttgtc agcaacggag gaagctctct cggttatgga gaaaccggcg
     1441 tcgggaaggt tagcatcacc acggggggga tatgggaggt caataagaat gtctatacca
     1501 ccattggtgt tgcgggcgtc ggaaacctca atattagcga tggcggtaag ttcgtatcgc
     1561 aaaatattac ttttttgggc gataaagcaa gcggtatcgg cacactgaac ctgatggatg
     1621 cgacatcgtc gttcgatact gtgggtatca atgtcggtaa ttttggtagc ggtatcgtaa
     1681 atgtcagtaa tggtgccacc cttaattcaa cgggctatgg atttatcgga ggaaatgcct
     1741 ccggtaaggg aatagttaat atttcaacgg atagtctctg gaatttaaag acgtcttcta
     1801 ctaacgccca attgctacag gtcggtgtat taggcacggg tgaactgaat attaccaccg
     1861 gaggtatagt taaagcgcgt gatacacaga tagctctcaa tgacaaaagt aagggcgacg
     1921 tgagggtgga tgggcagaac tctcttcttg aaacattcaa tatgtacgta gggacatctg
     1981 gtacgggtac gttaaccctg acgaatagcg gcacgctgaa tgtcgaaggt ggagaagttt
     2041 acttaggtgt ttttgaacct gctgtaggaa cgctaaacat tggtgcagct cacggtgagg
     2101 cggcggcaga tgccggattt atcaccaacg cgacgaaagt agagtttggc tctggcgaag
     2161 gtgtttttgt ctttaatcat actaataaca gtgatgccgg ctaccaggtc gatatgctga
     2221 ttacaggtga cgataaagac ggaaaagtga tccatgatgc aggccatacg gtgttcaatg
     2281 cagggaatac ttatagcggt aaaacgctgg tcaatgacgg cctcctgacc attgcgtctc
     2341 atacggcaga tggggtaacg ggcatgggat cgagtgaagt aaccattgca agccccggta
     2401 cgctcgacat tctcgcatca acgaacagtg caggagatta cacgctgacc aatgcgctca
     2461 aaggcgatgg cttgatgcga gttcagctgt catcctccga caagatgttt ggctttacac
     2521 atgcaacagg gactgaattc gccggtgttg cccaactgaa agacagtacc ttcactctgg
     2581 aacgcgacaa caccgctgcg cttactcacg cgatgttgca gtctgacatt gaaaatacca
     2641 catcggtaaa cgtgggagag caatccattg gtggactggc catgaatggc ggtacgctca
     2701 ttttcgatac ggatattcct gctgcgacgc ttgcagaggg atatatcagc gtcgatacgc
     2761 tggttgtcgg cgcgagtgac tacacctgga aaggccgtaa ctatcaggta aacgggacgg
     2821 gcgacgtgct tatcggcgtg cctaaaccgt ggaatgatcc tatggcgaat aaccctctga
     2881 cgacgctcaa tttgctggaa cacgatgata accatgtcgg cgttcaactg gtgaaggcgc
     2941 aaacggttat tgggtcgggt ggctcattaa cgttacgtga tttacagggc gacgaggtgg
     3001 aagcggacaa aacgttacac attgcgcaaa acggaacggt ggtcgccgag ggtgattatg
     3061 gattccgcct cacgaccgca ccaggtgatg gtttgtacgt taactatggg ctgaaagcgc
     3121 tgaacatcca tggagggcaa aagctgacgt tagccgaaca tggcggagcc tatggcgcaa
     3181 cggccgatat gtcggcaaaa atcggtggtg aaggggatct ggcaatcaat acggtgcgac
     3241 aggtttcgct ttccaacggt cagaacgact atcagggggc aacctacgtt cagatgggga
     3301 cattacgtac cgatgcggat ggcgcgctgg gcaacacccg ggaactgaac atcagcaacg
     3361 cagccatcgt cgatcttaat ggatcgacgc agacggtaga gacattcacc gggcagatgg
     3421 gttcgactgt tttgttcaaa gaagggtcgc tgacggtaaa taaaggtggg atcagtcagg
     3481 gtgaactgac aggtggcgga aacctgaatg ttacaggggg aacgctggct gtcgaggggc
     3541 ttaatgcacg ctacaatgcg ttaaccagcg ttagcccaaa tgcggaagtc agcctcgata
     3601 atacgcaggg gttaggcaga ggaaatattg ccaatgacgg tctgttaacg ctaaaaaacg
     3661 tgactggcga actgcgtaat agcataagcg ggaagggtat cgtgagcgca accgccagga
     3721 cagatgtaga gctggatggc gataatagcc gctttgtggg gcaattcaac attgatacag
     3781 gcagcgcgct cagcgtcaac gagcagaaaa acctgggtga tgcttccgtt atcaataatg
     3841 gcctgctcac catctccact gagcgtagct gggcgatgac gcacagtatc agcggtagcg
     3901 gtgatttgac aaaactgggt accgggatcc tgactcttaa caacgattcc tcggcgtatc
     3961 agggtacgac ggatatcgtg gggggggaaa ttgctttcgg ttccgactct gccattaata
     4021 cggcaagtca acacattaat atccataaca gcggtgtgat gtcgggaaat gtcaccactg
     4081 caggtgatgt gaacgttatg tctgggggga cactgcgtgt cgctaaaacc acaatcggcg
     4141 aatcggcggc aacctggaga atggcggcac ggttcaaatg aacagtgaag gaggaaaacc
     4201 ggggaatgta ctgaccgtta acggcaacta taccggaaac aatggcctga tgacgttcaa
     4261 cgcgacgctg ggcggcgata attcacccac cgataagatg aacgtgaaag gcgataccca
     4321 agggaacact cgcgttcggg ttgataacat tggcggcgtc ggtgcgcaaa cggtcaacgg
     4381 tattgaactc attgaggttg gcggtaattc tgcaggtaat ttcgcgctga ccaccggaac
     4441 tgtcgaagct ggggcttacg tctacacgct ggctaaaggg aaggggaatg acgagaaaaa
     4501 ctggtatctg accagtaaat gggacggcgt aacgccagcg gatacacccg atcccatcaa
     4561 taatccccct gttgtggatc cggaaggccc atcagtttat cgcccggagg ccggaagcta
     4621 tatcagcaac attgccgcag ccaactcgct gtttagccat cgcttacacg accgtctggg
     4681 tgagccgcaa tatacagatt cactgcattc tcaggattca gcaagcagta tgtggatgcg
     4741 tcatgtcggg gggcacgaac gttccagtgc cggagacggc cagctaaata ctcaggctaa
     4801 ccgctatgta ttgcagctag gcggcgattt ggcgcagtgg agtagcaacg cgcaggatcg
     4861 ctggcatctt ggcgtgatgg caggctacgc caatcagcac agtaatactc agagtaatcg
     4921 tgtgggttat aaatcggatg ggcgcatcag cggttacagc gctgggctgt acgcgacctg
     4981 gtatcagaac gatgcgaata agaccggcgc ttatgttgac agctgggcgc tgtataactg
     5041 gtttgataac agcgtcagtt ccgataaccg ttctgctgac gactatgatt ctcgcggtgt
     5101 gacggcctct gttgagggtg ggtatacctt tgaagcggga acatgtagcg gcagcgaagg
     5161 gacgctgaat acctggtacg tccagccaca ggcgcaaatc acctggatgg gtgtgaaaga
     5221 ttctgaccat gcccggaaag acggaacgcg cattgaaacg gaaggcgacg gaaacgtgca
     5281 aacgcgactt ggggtgaaaa cctacctgaa tagccatcac cagcgtgacg atggtaaaca
     5341 gcgtgagttc cagccttaca ttgaagcgaa ctggatcaac aatagcaaag tctacgccgt
     5401 gaagatgaat ggtcaaaccg taagccgtga tggtgcgcga aatctcggtg aagtacgtac
     5461 cggggttgag gcgaaagtaa ataacaacct tagcctgtgg gggaatgtcg gtgtgcaact
     5521 aggtgataaa ggctatagcg atactcaggg catgctggga gtgaaatata gctggtaaac
     5581 agtataagcc gcatgtcgag atggcatgcg gcttaatatt gccaacttca aaaggctttt
     5641 aaagcctccc ttttatccgc gatcgcggat atcgcagcgt ttatcccgta cagtttgatt
     5701 tattgtcaga aatagggcga gacagcgttg gtgccgtgac gttaataccc gaagacgaaa
     5761 ccgtaacgcg tccgataatg gcatgggaaa agcttactga agtcagactt gaagaagtat
     5821 taacggctta taaagcagat atcccgctag gcatgattag agaagaaaat gactttcgca
     5881 tctcggttgc tggcgcacag gagaagacag cactgctcag aataggcaat gactggtgca
     5941 ttccgaaagg aataacgccg acgacgcaca tcattaaatt accgattggg gaaatcaggc
     6001 agcccaatgc gacgctcgat ctcagccaaa gcgttgataa tgagtattac tgtctgctgc
     6061 tggcgaaaga acttgggttg aatgtcccgg acgcagaaat cattaaagcg ggaaatgtgc
     6121 gcgcgttagc ggtcgaacgt tttgacaggc gttggaatgc tgagcgaacg gttttacttc
     6181 gcttgccaca ggaggatatg tgtcagacat tcggtttacc ttcatcggtg aaatatgaat
     6241 cagatggagg cccaggcatc gcgcggatca tggctttttt gatggggttc agcgaggcgc
     6301 ttcgcgatcg ttatgatttt atgaaattcc aggtcttcca gtggttgatt ggcgcaacgg
     6361 atggccatgc aaaaaacttc tccgtattta ttcaggctgg cggcagttat cgactcacgc
     6421 cattttacga catcatttca gcatttccgg tccttggcgg tacgggaata cacatcagcg
     6481 atctcaaact ggcaatgggg cttaacgcat ccaaaggcaa aaaaacggca atcgataaaa
     6541 tttatccgcg acattttttg gcgacagcaa aggtgctgag attcccggaa gtgcagatgc
     6601 atgaaatcct gagtgacttt gccagaatga ttccggcagc actggataac gtgaagactt
     6661 cattaccgac agattttcca gagaacgtgg tgacggcagt tgaaaccaat gtgttgaggt
     6721 tgcacggtcg gttaagccga gaatacggta ttaagtaaga gtaagatatg gggtttggtc
     6781 gttgttaatg agcaggacaa tcatgaccgc caacgactag attctccctt ggtttatatt
     6841 ggtgcgcata atgtatatta tgttaaatcg tatctgagtg atacgaagtg attttccggt
     6901 agtcgttatt cgttcaggat tccagaactt aagcttctcc tctttcataa ttggcctgtg
     6961 catgacggat ataggctggc tggtatgccg atttgccgtt tgccgcatct tcccagcgtt
     7021 gaagactcaa gctggcaaac cgcgtgcaaa tgagcacgcc ctctttccat caacacttca
     7081 ggcagcttgc gggaatgagt ccactccggt atcagaaatg gctacgtctc aatgaagtca
     7141 ggcgaacgat gctgaatgaa cattacgatg tcaccacagc agcctatgct gtcggttacg
     7201 aaagcttatc ccatttccga cgggaatatt tgcggatgtt tggagagtca cccaagagag
     7261 atattaccag gttaagaaag tctgtcggtc agctttaata aaccctgcac ttatctgttt
     7321 agagcaattc ggtgttagtt acaccaggca aacattaact atagctaatg atttatagcc
     7381 atataaacca ttagtagccc ttgacctttc ctgacacttc ccttgttcgc aataatattc
     7441 tgtttagtca cggtttataa ataacatgca taatgaaatc gtcatgttat ttagcgacat
     7501 aaaatgtttt tgcaaatact ctcttcaaca agtgcagcaa taaacttgtt gtgattaggt
     7561 gtccttgtga tttaaagata ttatcgttct tcgtgaaatg taataaccct ggaaactata
     7621 ttctcagggc aacaaccaat caagaatgat tcttgtttta taagcaataa cagaatgact
     7681 taattgttaa ctctctttat tcagctactt aaatataaat tttggagaat tatgaaactc
     7741 aaacatgttg gtatgattgt cgtttctgtg ttggcgatgt cgtctgctgc ggtaagcgca
     7801 gccgagggtg atgaatcagt aacgaccact gttaatggcg gtgttattca ttttaaaggt
     7861 gaagtggtaa atgccgcttg tgcgattgat tccgaatcaa tgaaccaaac ggttgagctg
     7921 ggtcaggttc gttcttctcg cctggctaaa gcgggtgacc tcagctccgc cgttggcttc
     7981 aatatcaagc tgaatgattg tgataccaat gtttccagta atgcagctgt tgcattcctg
     8041 ggtactactg tcaccagtaa tgacgatacg ttagcgctgc agagttcagc ggcaggctct
     8101 gcccaaaatg tcggtattca aattttggac cgtacgggtg aggtattaat acttgatggg
     8161 gcctctttta gtgctaaaac cgacttgatt gatggcacga atatactacc attccaggct
     8221 cgttatattg ctctcgggca gtccgtagct ggtactgcaa acgcagatgc gaccttcaaa
     8281 gttcaatatc tataattcaa tattaaggtg gctatacggc atctgaaatt aaaacagggt
     8341 gtgtgtggca tggctacgca cacccgagca attcaccaat gtgaatctca ttattaatga
     8401 tgagtggttt gtatttggag cgctatgaaa aatcacataa tatctaaacg ttcaataaac
     8461 aacagtagtt aaacttactg ttattaaaaa tatttacttt ttagcaaggt tttcctatta
     8521 aaagagaacc aacctttatt acaaacgcat taagtctgga aacccggtga ctcgccaatg
     8581 tattagcaac atttaccgat tgtctgatgt aacggttttc ccgcttatcc tcttaacaca
     8641 tggagaatgc atcagatgca aactacgcgt accccctact ctatttcctt tatggcgaca
     8701 gtgttacttt tattgctctt tgcttgccat agcaccgttg ccaacgcagc tgtcgcactg
     8761 ggtgcgacgc gggtaattta tccggcaaac cagaaacagg ttttattgcc tgtcacgaat
     8821 aatgatcctg ccagtgtcta tctcattcag tcatggatag aaaatgctgg tgaccaaaaa
     8881 gacacccagt ttgtcattac cccacctctt ttttccatgc aaggtaaaaa agagaatacg
     8941 ctgcggataa tcaacgccac taatcatcag ttacccggcg accgtgaaag cctgttctgg
     9001 gttaatgtta aagctattcc tgctatggaa aaagaccaga agaatgagaa tacattacag
     9061 ctggcaatta ttagccgcat aaaaatgttt tatcggccca cgaacctggc aatggcacct
     9121 gaagaagcgc ctgcaatgct tcggtttcgt cgcagcggta gcaatctcac tcttattaat
     9181 ccaacgccct acttcattac cgtaacaaat atgaaagcag gaaatagcaa tctcccaaac
     9241 accatggtgc cgccaaaagg tgaagtgtcg gtggatatcc cgcatgctgc caccggggat
     9301 atcagttttc agacgatcaa tgattacggc gcattaactc cgcgtattaa ggcaaccatg
     9361 cagtaagcca gtgacctaat taataataaa ctcaatcgga attaataatg acagcctttc
     9421 gtgccgcatt taaggcatat cgtatgcatc aggtgttact actgcctcgc tttgcacggc
     9481 ttaccattgc ccttagctta gcaactgcgg tatttcccgt tgacgcagag tattatttta
     9541 atccacggtt tttaagcaat gatcttgctg aatctgtcga tttatccgcc tttactaagg
     9601 gacgcgaggc accacctggt acatatcggg tcgacattta tttgaacgac gaatttatga
     9661 ctagtcggga tattactttt attacagatg ataataacgc agatctcatc ccatgcctga
     9721 gtacagatct tcttgtcagt cttggcataa aaaaatctgc gttattagac aataaggaac
     9781 attcagcaga aaaacatgtg ccggacaaca gcgcatgcac accacttcag gatcgtttgg
     9841 tcgacgcatc aactgagttt gatgttggtc agcaacatct gtcactctcc gttccgcaaa
     9901 tttatgttgg cagaatggct cgcggctatg tttctcctga tctgtgggaa gaaggaataa
     9961 atgctgggct tctaaactat agttttaatg gtaattctat taataatcgt agtaaccata
    10021 atgcaggaaa atccaactat gcatatttga atttacagag tggcatcaac attggtagtt
    10081 ggcgactacg cgataactca acgtggagtt ataacagtgg gagtagcaat tcatctgaca
    10141 gcaataaatg gcagcatatc aatacgtcgg ctgaacgtga cattattccc ttacgctcac
    10201 ggttaacggt aggtgatagt tataccgatg gcgatatttt tgatagtgtg aactttcgtg
    10261 gcctcaaaat aaattcaaca gaagcgatgt tgcccgatag ccaacatggt ttcgctccgg
    10321 tgattcatgg tattgcccgt ggcaccgcac aagtgagtgt aaaacagaac ggatacgatg
    10381 tttatcagac tactgttcca cccggccctt ttactattga tgatatcaac tctgcggcca
    10441 atggtggtga tctgcaagta accataaaag aggcagacgg cagtattcag acattatatg
    10501 ttccttattc gtctgttccg gttctccaac gtgctggata tacgcgttat gcgcttgcca
    10561 tgggggaata tcgtagtgga aataacctgc aaagctcccc caggttcata caaggtagct
    10621 tgatgcatgg actggaagga aactggacac cttatggcgg aatgcaaatt gcagaagatt
    10681 atcaggcctt caaccttggt attggtaaag atttaggact ttttggtgcc ttttctttcg
    10741 atatcacgca ggccaatacg acacttgcag atggcacccg tcacagcggg caatcggtta
    10801 aatccgtcta cagcaaatcc ttctaccaga caggaaccaa tatccaggtc gcaggatatc
    10861 gctattctac gcaaggtttt tataacttat ccgacagtgc ctacagtcga atgagtggtt
    10921 acaccgtcaa gcctcctacc ggagacagca atgagcagac acaatttatt gattatttta
    10981 atctgttcta cagtaagcgt ggtcaggaac aaataagcat ctctcagcag cttggaaatt
    11041 acggtgcgac atttttcagt gccagtcgcc aaagttactg gaacacgtca cgcagcgacc
    11101 agcaaatatc atttggatta aatgtgccgt ttggtgatat tacgacttcg ctgaattaca
    11161 gctattccaa taatatatgg caaaacgatc gggatcattt actcgctttt acgcttaatg
    11221 ttcccttcag tcattggatg cgtacagaca gtcagtcggc atttcgtaat tcaaacgcca
    11281 gttacagtat gtcaaacgat ttgaaaggcg gcatgaccaa tctatcgggg gtttatggca
    11341 ctctgctgcc ggataataac ctgaattata gcgttcaggt cggtaacacc cacggaggta
    11401 atacatcgtc tggcaccagt ggttacagta ctcttaatta tcgtggagct tacggcaata
    11461 ctaatgtcgg ttacagtcgg agtggtgaca gcagccagat ttattacgga atgagtggtg
    11521 ggattattgc tcatgctgat ggcatcacct ttggacagcc gctgggcgac acaatggttc
    11581 tggttaaggc tcctggcgct gataatgtca aaatagagaa ccagaccgga attcataccg
    11641 actggcgtgg ctatgccata ttaccatttg cgacagaata tagagaaaat cgtgtcgctc
    11701 ttaacgcgaa ttcccttgca gataatgttg aactggatga aaccgtggtc actgtcatcc
    11761 caactcacgg tgctattgcc agagcaacat ttaatgcaca aatcggcggg aaagtattaa
    11821 tgacgttgaa gtacggtaat aaaagcgttc cattcggtgc aattgtcact cacggagaga
    11881 ataaaaatgg cagcattgtc gcggaaaacg gtcaggttta tctgactgga cttccacagt
    11941 cagggaaatt acaggtttca tggggcaatg ataaaaactc aaactgtatt gtcgattaca
    12001 agcttcctga agtctctcct ggaaccttgc tgaaccagca gacagcaatc tgtcgctaag
    12061 gaacaataga ttatgaaata caataacatt attttcctcg gtttatgtct ggggttaacc
    12121 acctattctg ctttatccgc agatagcgtt attaaaatta gcgggcgcgt cctcgattat
    12181 ggctgcacag tctcatcgga ttcgcttaat tttaccgtag atctccaaaa aaacagtgcc
    12241 agacaatttc caacgaccgg tagcacaagt ccagccgtcc cttttcagat tacgttaagt
    12301 gaatgcagca aagggacaac gggggttcgg gttgcattta acggtattga ggacgcagaa
    12361 aataatactc tgttgaaact ggatgaggga agcaatacgg cctccggttt aggtatagaa
    12421 atactggacg gaaatatgcg tccggtgaaa ctgaatgacc ttcatgccgg gatgcagtgg
    12481 atcccactgg taccagaaca gaacaatatt ttgccttact ccgctcgtct gaagtcaact
    12541 cagaagtccg tcaatccggg actggtgagg gcttcggcaa cctttaccct tgaatttcaa
    12601 taatcagagt cagatatgaa aagacttcac aagaggttcc tcttagctac gttttgcgcg
    12661 ttattaacag caactctcca ggccgccgat gtcactatca ctgttaatgg tcgggtagtc
    12721 gctaaaccct gcactattca aaccaaagaa gctaacgtta atctcgggga tctttatacg
    12781 cgcaatctgc aacaacctgg ttctgcatct ggctggcaca atattacttt gtcattaacc
    12841 gattgtccgg ctgaaacaag tgcagtgacg gcaatcgtga caggttcaac tgacaatacg
    12901 ggttattaca aaaatgaagg tactgccgaa aatattcaga tagagcttag ggatgaccag
    12961 gatgcgacgt taaaaaatgg cgatagcaaa acggttattg ttgatgagat cactcgtaat
    13021 gcacagtttc cacttaaggc aagagctatc acggtgaatg gaaacgcaag ccagggaacg
    13081 atcgaggcgc taatcaatgt gatctacacc tggcaataac gtcgattcga ttttcttagc
    13141 cttcttttta aatcaatttt ttaaccttag gatatatcat gggtaaaaca atcagtataa
    13201 aggtcctgtt cggtatttat cttctattaa tggcaggcaa agtttttgcc ttcagttgta
    13261 atgtcgatgg cgggtcaagt attggagctg gtacaacctc tgtttatgtt aatctcgacc
    13321 ctgtaataca gccgggccag aatctggttg tagacttgtc tcagcatata agttgctgga
    13381 atgattacgg cggctggtac gacactgatc atataaacct ggtacaaggc tcagctttcg
    13441 caggctctct acagtcatat aaaggatcgc tgtactggaa taatgtgacc taccccttcc
    13501 cgttgacgac aaataccaac gtattggata ttggtgataa gacaccgatg ccattaccgt
    13561 taaaactcta tattacccca gtcggggcgg ccggtggagt tgtcataaaa gccggggaag
    13621 ttattgcccg tattcatatg tataaaattg ccacgttagg aagcggaaat ccgcgtaatt
    13681 ttacgtggaa tattatttca aataacagtg ttgttatgcc aacaggcggc tgcactgttg
    13741 atagtcgtaa tgtcaccgtg aatctcccgg actttcctgg aagcgcagaa atcccccttg
    13801 gcgtatattg ctcaagcgaa caaaagctct cattttatct ttcgggtact accactgaca
    13861 gtgctagaca agtgtttgct aacacagcac cagatgcaac aaaagcatcg ggagtgggtg
    13921 tctctctgat gcgtaatgga aaaatactgg ctacgggaga aaatgtctcc ctggggacag
    13981 taaataaaag caaagtacct ctgggtttaa gcgcaaccta tggacaaaca gggaacaaag
    14041 tttcagccgg aaccgtccag tcagtcattg gtgtcacttt tatctatgaa taattgtgat
    14101 tttgacctta gtctatgcgc tccattcacg aaaattgcgc ggataattat tttgtgaagg
    14161 ctattatcct acacttgtaa gaaaatccgc gcacaattga agccagagtc ttatttttta
    14221 tattgaaaaa tatgttaatt taaacactat gagagatgat gcttacctta tcgtatttaa
    14281 tctattgttt aacgataata gattaaaaat agaaagagag tagtccccta ctcttatata
    14341 tccatgttgg cgataatccc tttgtatgta ctgtgcatca tcgctattac aaatcctaat
    14401 aattcatttc cacacaggat aagtagatga agaaaaaaat tgaatcctac cagggtgctg
    14461 ccggtggttg gggtgctgtt aaatccgtag cgaatgcagt acgtaagcag atggatatac
    14521 gccaggatgt tattgccatg tttgacatga ataagccaga gggctttgac tgtccgggtt
    14581 gtgcatggcc agatcctaag cacagtgcgt cattcgacat ttgtgaaaac ggcgcaaaag
    14641 caatcgcctg ggaagtcacg gataagcagg taaatgcctc tttctttgct cagaatacgg
    14701 ttcaatcatt acttacctgg ggagatcacg agcttgaggc tgcgggacgt ctcactcagc
    14761 ctttgaaata tgatgctgtc agcgactgtt acaagccatt aagctggcaa caagctttcg
    14821 acgaaattgg cgcacgcctt caaagctata gtgatcccaa tcaggttgaa ttctatactt
    14881 cgggccgcac ttccaatgaa gctgcctttc tttatcagct ttttgcccgt gaatacggga
    14941 gcaataactt tcccgactgc tccaacatgt gccatgaacc gacaagcgtg ggtttggcag
    15001 cgagtatcgg tgtaggtaaa gggaccgtgt tgctggaaga ctttgagaag tgcgatttag
    15061 tcatttgcat tgggcataac cctggtacaa accaccctcg catgctgact tcgttgcgcg
    15121 ctttagtgaa acggggagcg aaaatgatcg ccatcaatcc tctacaggaa cgtggcctgg
    15181 agcgatttac cgcaccgcaa aacccgtttg aaatgctgac gaactctgag actcagttgg
    15241 ccagtgccta ctataacgtg cgcattggtg gtgatatggc gttgctcaag gggatgatgc
    15301 gcctgttaat tgagcgcgat gatgctgcaa gcgccgcagg tcggccctca ttgctggatg
    15361 acgaatttat tcaaacgcat accgtcggct ttgacgagct acgccgtgac gtgctcaatt
    15421 ccgagtggaa agatatcgaa cgtatttctg gactaagtca gacacaaatc gccgaactgg
    15481 ctgatgcata tgccgctgcc gaacgaacca ttatctgtta cggaatgggg atcactcagc
    15541 acgaacatgg tacccagaat gtacagcaac tggtcaatct gctgttgatg aaaggtaaca
    15601 ttggcaagcc tggtgcgggt atctgcccac tacgtggcca ctctaatgta cagggcgacc
    15661 gaaccgtcgg tatcaccgag aaaccgtctg tagagtttct ggctcgcctg ggtgagcgct
    15721 atggcttcac cccacctcat gtacctggac atgctgcaat tgccagcatg caagcaatat
    15781 gtacggggca ggctcgagca ttgatctgca tggggggcaa ctttgcactg gcaatgccag
    15841 atcgggaagc gagcgctgta ccgttaacgc aattagattt ggcggtacac gtagccacta
    15901 agcttaaccg ctctcatctg ctgaccgcac ggcatagcta tattctgccg gttctgggac
    15961 gtagcgagat tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag gattcaatgt
    16021 cgatgattca tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt
    16081 gtgctgtggt cgcgggaatc gcgcaggcaa cactacccca gagcgtggta gcctgggagt
    16141 atctggtgga agattatgat cgcattcgca atgacattga agctgtgctg ccagagttcg
    16201 ccgactataa ccaacgcatc cgtcatcccg gtggttttca cctgataaat gcagctgctg
    16261 aaaggcgctg gatgacgccg tcaggtaagg ctaatttcat taccagcaaa gggctgttag
    16321 aagatccctc ttcagcgttt aacagtaagc tggtcatggc gacagtacgc agccacgatc
    16381 agtacaacac gacgatttat ggtatggatg atcgctatcg aggggtattc ggtcaacgag
    16441 atgtggtctt tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag
    16501 ttaatcttat tgcgcttacg ccagacggta agcgcagctc acgccgcatg gatagattaa
    16561 aagtggtcat ttaccctatg gctgaccgct cactggtgac ctattttcca gaatcgaatc
    16621 acatgctaac acttgataac cacgatccat taagtggcat tcctggctat aaaagtattc
    16681 cggttgaatt agaaccatca aattaatgcc tcttctcatt tcttctgctg tcatccgcac
    16741 agcagaagaa ttcctcattg attattatcg cgcaatttgc tcacatggat taaattaaac
    16801 tacatactat aagatataaa cttctgccta cagctgtaag aaactccgct cagtactgaa
    16861 gcaccagtcc tatttcctct tttctcaagc ctgttatatt aagcatactg attaacgatt
    16921 tttaacgtta tc
//
LOCUS       DAZFTN010000097        16885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFTN010000097 DAZFTN010000000
VERSION     DAZFTN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..187
                     /gene="kil"
                     /locus_tag="ACHKV2_003666"
     CDS             <1..187
                     /gene="kil"
                     /locus_tag="ACHKV2_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6941475.1"
                     /translation="RQCVTPGDYVLHEGRTYIASANNIKKRKLYIRSLTTKTCISDCM
                     IKVFLGRDGLPVKAESW"
     gene            187..357
                     /locus_tag="ACHKV2_003667"
     CDS             187..357
                     /locus_tag="ACHKV2_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO6941476.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            438..3107
                     /gene="recE"
                     /locus_tag="ACHKV2_003668"
     CDS             438..3107
                     /gene="recE"
                     /locus_tag="ACHKV2_003668"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO6941477.1"
                     /translation="MSTDKENYALYCDAKNDKTRKRLGIKGGFFWTEAKKLSVAVSRC
                     IAAMDDAGFDEEDFKKPIRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMILIAPS
                     VPDKPASTDNTNVNGEDMAKIEENMLLPVSGQILPVRWLAQHNSEKPLTHVSLDKLRA
                     LHNAQDEKLPAVTALAISNKAVQLEPLEIRDLYKLVRDTDKVFPAPVNSDLGLMTSFI
                     EAYLGADYTDRGLLTKEWMKGNRVSRISRTPSGANAGGGNITDRGEGFKHDKTSLARD
                     VATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVV
                     ASVKEAPIGIEVIPAHVTEYLNKVLIETDHANPDPEIVDIACGRSSAPMPQRVTEEGK
                     HDGEEKPQPSCAMADKQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKYQKLRAELH
                     EARKTIPPKNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQND
                     QKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMGDATYQET
                     FDEENQVEAKENDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYY
                     GISNENYHVGPGVSKSQLDDIADTPALYLWRKNAPVDNTKTKTLDLGTAFHCRVLEPE
                     EFSNRFIVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGLKIELMYQSVMALPL
                     GQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYD
                     YRYHVQDAFYSDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYH
                     RNLRTLADCLNTNEWPAIKTLSLPRWAKEYAND"
     gene            3100..3909
                     /gene="recT"
                     /locus_tag="ACHKV2_003669"
     CDS             3100..3909
                     /gene="recT"
                     /locus_tag="ACHKV2_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO6941478.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            3966..4160
                     /gene="ralR"
                     /locus_tag="ACHKV2_003670"
     CDS             3966..4160
                     /gene="ralR"
                     /locus_tag="ACHKV2_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO6941479.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            4153..4341
                     /locus_tag="ACHKV2_003671"
     CDS             4153..4341
                     /locus_tag="ACHKV2_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO6941480.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            4441..4656
                     /gene="xisR"
                     /locus_tag="ACHKV2_003672"
     CDS             4441..4656
                     /gene="xisR"
                     /locus_tag="ACHKV2_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO6941481.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            4658..5893
                     /gene="intR"
                     /locus_tag="ACHKV2_003673"
     CDS             4658..5893
                     /gene="intR"
                     /locus_tag="ACHKV2_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6941482.1"
                     /translation="MSKLPTGVEIRGRNIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISYIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGTVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKVQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            5945..6880
                     /gene="ttcA"
                     /locus_tag="ACHKV2_003674"
     CDS             5945..6880
                     /gene="ttcA"
                     /locus_tag="ACHKV2_003674"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO6941483.1"
                     /translation="MQENQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7009..8382)
                     /gene="dbpA"
                     /locus_tag="ACHKV2_003675"
     CDS             complement(7009..8382)
                     /gene="dbpA"
                     /locus_tag="ACHKV2_003675"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO6941484.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(8415..8585)
                     /gene="ynaL"
                     /locus_tag="ACHKV2_003676"
     CDS             complement(8415..8585)
                     /gene="ynaL"
                     /locus_tag="ACHKV2_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO6941485.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(8860..9843)
                     /gene="zntB"
                     /locus_tag="ACHKV2_003677"
     CDS             complement(8860..9843)
                     /gene="zntB"
                     /locus_tag="ACHKV2_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO6941486.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(9857..9904)
                     /gene="ydaN"
                     /locus_tag="ACHKV2_003678"
     CDS             complement(9857..9904)
                     /gene="ydaN"
                     /locus_tag="ACHKV2_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO6941487.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10098..11330
                     /gene="dgcM"
                     /locus_tag="ACHKV2_003679"
     CDS             10098..11330
                     /gene="dgcM"
                     /locus_tag="ACHKV2_003679"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO6941488.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(11351..11914)
                     /gene="smrA"
                     /locus_tag="ACHKV2_003680"
     CDS             complement(11351..11914)
                     /gene="smrA"
                     /locus_tag="ACHKV2_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO6941489.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12244..13152)
                     /gene="abgR"
                     /locus_tag="ACHKV2_003681"
     CDS             complement(12244..13152)
                     /gene="abgR"
                     /locus_tag="ACHKV2_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6941490.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            13328..14638
                     /gene="abgA"
                     /locus_tag="ACHKV2_003682"
     CDS             13328..14638
                     /gene="abgA"
                     /locus_tag="ACHKV2_003682"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO6941491.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            14638..16083
                     /gene="abgB"
                     /locus_tag="ACHKV2_003683"
     CDS             14638..16083
                     /gene="abgB"
                     /locus_tag="ACHKV2_003683"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO6941492.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            16120..>16885
                     /locus_tag="ACHKV2_003684"
     CDS             16120..>16885
                     /locus_tag="ACHKV2_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFO6941493.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLT"
BASE COUNT         4650 a         4090 c         4135 g         4010 t
ORIGIN      
        1 acgtcagtgt gtgactcctg gcgattatgt tcttcatgaa ggtcggacat atatcgcctc
       61 ggcaaacaat attaaaaagc gaaaacttta tattcgtagc ctgactacaa aaacatgcat
      121 ttctgactgc atgattaaag tcttcctcgg tcgtgatggt ttacctgtaa aggcggagtc
      181 atggtgatga ctaagaaaat aaaatgtgct taccaccttt gcaataaagg aattgaagaa
      241 agcaaaagta ttaaaagacc acttcatttc atgcgtggag ttatcccaac gacggaaatg
      301 aaaaaatatt gtagtgaaaa ttgtgccgaa aaagaccaga tggcacacga actttaacta
      361 actgactatt cgaaactgaa tttatgccag caatggcagg ggttcgctca accttaaaat
      421 agttatgagg tttatcaatg agtactgata aagaaaatta cgctctgtat tgcgacgcga
      481 aaaatgataa aaccagaaaa cgcctcggca tcaaaggcgg ctttttctgg acagaggcaa
      541 aaaaactttc tgttgcggtt tcacgctgca ttgctgcaat ggacgatgcc ggatttgacg
      601 aagaggattt taaaaaaccc atccgcgtcc atttacccgt tgtgaatgac cttccaccgg
      661 aaggtgtgtt tgatactgaa ttctgcaacc gatacgaaaa aggcggagaa gatggtatca
      721 caatgatact tatcgcgccc tctgttccgg acaaaccagc cagtactgac aataccaacg
      781 tcaacggcga agacatggct aagattgagg agaatatgct tcttccggtt tcaggtcaga
      841 ttctgcctgt tcgctggctg gcgcaacaca acagcgaaaa accgctcacg cacgtttcac
      901 tggacaaact gcgcgcatta cataacgcac aggatgaaaa acttcccgcc gttaccgcgc
      961 tggccatctc aaataaagca gtgcaactcg aaccgctgga gattcgggat ttgtacaaac
     1021 tggtgcgaga cactgacaaa gttttccccg cccccgtaaa ttcggacctg ggactgatga
     1081 cctcttttat cgaagcatac ctgggcgctg actacaccga tcgcggtctg cttacaaaag
     1141 agtggatgaa aggaaatcgt gtttcacgca tcagccgcac gccatccggc gctaacgctg
     1201 gcggcggaaa tattaccgat cgtggtgaag gattcaaaca tgataagaca tcactcgcac
     1261 gagatgtagc caccggcgtt ctggcccgtt caatggatgt ggatatttat aaccttcatc
     1321 cggcacacgc taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt
     1381 tccgcgacaa attcatcacc atgcctggcg ggctggatta ttcccgcgcc atcgtggttg
     1441 cgtccgtaaa agaagcacca attgggatcg aggttatccc tgcgcatgtc actgaatatc
     1501 tgaacaaggt gctgattgaa accgatcatg ccaaccctga tccggaaatc gtggatattg
     1561 cctgcggtcg ttcctctgcc ccgatgccgc agcgtgtaac agaagaagga aaacacgatg
     1621 gtgaagaaaa accgcaacca tcttgcgcaa tggcagataa acaggcaacg gctgaaacag
     1681 tggaaccgga tgcaactgaa catcatcagg acacgcagcc gctggatgct cagtcacagg
     1741 taaattctgt tgatgcgaaa tatcagaaac tgcgggcaga actccatgaa gcccggaaaa
     1801 ccattccgcc caaaaatcct gtcgatgccg acaaattgct ggctgcctct cgcggagaat
     1861 ttgttgaagg gattagcgac ccgaatgatc cgaaatggat taaggggatc cagacccgcg
     1921 attctgtgta ccagaatcag ccagaaacgg aacagaacga ccagaaagcg gaacagaaca
     1981 gcccaaatac gcaacaaaac gagccagaaa cgaaacaacc tgaaccagta gtgcaacagg
     2041 aaccggaaaa gatctgcacc gcctgcggcc agaggagtgg cggcaactgc cctgattgtg
     2101 gcgcggtgat gggcgacgca acataccagg aaacattcga tgaagagaat caggttgaag
     2161 ctaaggaaaa tgatccggag gaaatggaag gcgctgaaca tccgcacaat gagaatgctg
     2221 gcagcgatcc gcatcgcgat tgcagtgatg aaactggcga agtcgcagat cccgtaatcg
     2281 tagaagacat agagccaggt atttattacg gaatttcgaa tgagaattac cacgtaggtc
     2341 ccggtgtcag taagtctcag ctcgatgaca ttgctgatac tccggcactg tatttgtggc
     2401 gtaaaaatgc ccccgtggac aacacaaaga caaaaacgct cgatttagga accgctttcc
     2461 actgccgggt acttgaaccg gaagaattca gtaaccgctt tatcgtagca cctgaattta
     2521 accgccgtac aaacgccgga aaagaagaag agaaagcgtt tctgatggaa tgcgcaagca
     2581 caggaaaaac ggttatcact gcggaagaag gtctgaaaat tgaactcatg tatcaaagcg
     2641 ttatggcttt gccgctgggg caatggcttg ttgaaagcgc cggacacgct gaatcatcaa
     2701 tttactggga agatcctgaa acaggaattt tgtgtcggtg ccgtccggac aaaattatcc
     2761 ctgaatttca ctggatcatg gacgtgaaaa ctacggcgga tattcaacga ttcaaaaccg
     2821 cttattacga ctaccgctat cacgttcagg atgcattcta cagtgacggt tatgaagcac
     2881 agtttggagt gcagccaact ttcgtttttc tggttgccag cacaactatt gaatgcggac
     2941 gttatccggt tgaaattttc atgatgggcg aagaagcaaa actggcaggt cagctggaat
     3001 atcaccgcaa tctgcgaacc ctggctgact gcctcaatac caatgaatgg ccagctatta
     3061 agacgttatc actgccccgc tgggctaagg aatatgcaaa tgactaagca accaccaatc
     3121 gcaaaagccg atctgcaaaa aactcaggga aaccgtgcac cagcagcaat taaaaataac
     3181 gacgtgatta gttttattaa ccagccatca atgaaagagc aactggcagc agctcttcca
     3241 cgccatatga cggctgaacg tatgatccgt atcgccacca cagaaattcg taaagttccg
     3301 gcgttaggaa actgtgacac tatgagtttt gtcagtgcaa tcgtacagtg ttcacagctc
     3361 gggcttgagc ccggtagcgc tctcggtcat gcatatttac tgccttttgg taataaaaac
     3421 gaaaagagcg gtaaaaaaaa cgttcagcta atcattggct atcgcggcat gattgatctg
     3481 gctcgccgtt ctggtcaaat cgccagcctg tcagcccgtg ttgtccgtga aggtgacgag
     3541 tttagcttcg aatttggcct tgatgaaaag ttaatacacc gcccgggaga aaacgaagat
     3601 gccccggtta cccacgtcta tgctgtcgca agactgaaag acggagggac tcagtttgaa
     3661 gttatgacgc gcaaacagat tgaactggtg cgcagccaga gtaaggctgg taataacggg
     3721 ccatgggtaa ctcactggga agaaatggca aaaaaaacgg ctattcgtcg cctgtttaaa
     3781 tacctgcctg tctcaattga aatccagcgt gcagtatcaa tggatgaaaa ggaaccactg
     3841 acaatcgatc cggcagactc ctctgtatta accggggaat acagtgtgat cgataattca
     3901 gaagaataat tcagcctggc ggtgtaatgc accgccaacg cgagacagtt tttatgacaa
     3961 aaattatgag atatgacgat gttaaaccat gtccgttttg tggttgtcca tctgttacgg
     4021 tgaaagcaat ttcaggatat taccgggcaa aatgcaacgg atgcgaatcc cgaactggct
     4081 atggtggaag tgaaaaagaa gcactcgaaa gatggaataa acgaactact ggaaataata
     4141 atggaggtgt tcatgtataa aataactgcc actattgaaa aagaaggtgg cactcctact
     4201 aactggacaa gatattcaaa atctaaatta acgaaatcag aatgcgaaaa aatgctctca
     4261 gggaaaaaag aagcaggcgt gtccagagag cagaaagtaa agctgataaa ttttaattgc
     4321 gagaaacttc tgtcctcgtg agttgcatta tatacaaatt aaaacttcat agctgattat
     4381 taataatcaa catcaggcgt caatttcagt ctaacattgg cgcctgccag aggtgatgcg
     4441 atggcacaag taatctttaa tgaagagtgg atggttgaat acggcctgat gcttcgcact
     4501 ggtctggggg ccagacaaat tgaagcatac cgccagaact gttgggtgga gggcttccac
     4561 ttcaaacgag tatctccttt aggtaagcca gacagcaaac gagggattat ctggtacaac
     4621 tatccaaaga taaatcagtt tatcaaagac tcatgatatg tctaaattac caacaggtgt
     4681 cgagattaga ggtagaaaca ttcgcatctg gttcatgttt cgaggaaaac gatgtcggga
     4741 aacattaaaa ggctgggaga ttacaaacag taatattaaa aaggccggaa atttaagagc
     4801 gctgatagtt catgaaataa actccggtga atttgagtat ttaagacgtt ttccccagtc
     4861 cagcactggg gcaaaaatgg tgacaacgag agtcataaaa acgttcgggg agctttgtga
     4921 tatctggaca aaaattaaag agacagagtt aacaacaaac acaatgaaga aaacgaaatc
     4981 acaattaaaa acactcagaa taataatttg tgaaagtacc ccgatatcat atattcgtta
     5041 tagcgatatc ttaaactacc ggaatgaact gctgcatgga gaaacgcttt acctggataa
     5101 tccaagatcc aacaaaaaag gaagaaccgt gcgcacagtt gataactata tcgccctgct
     5161 ctgttcgctg ttacgttttg cgtatcagtc gggatttata tcaaccaaac catttgaagg
     5221 agtaaaaaaa ttacagcgaa acagaataaa gcctgacccg ttatctaaaa cagaattcaa
     5281 tgcattaatg gaaagtgaaa aaggacagag ccaaaacttg tggaaatttg ccgtttactc
     5341 cgggcttcgt cacggggaac tggcagctct ggcgtgggag gatgtggatt tcgagaaggg
     5401 aactgtgaat gtcagaagaa acctgacgat actggatatg ttcggtcccc caaaaacaaa
     5461 tgccgggatc cggacagtaa cattactgca gcctgctctt gaagcactga aggtgcaata
     5521 caaactgacc gggcatcatc gcaaaagcga aatcaccttt tatcatcggg agtacggcag
     5581 aaccgaaaag caaaaactgc attttgtttt catgcccagg gtgtgtaacg gaaaacagaa
     5641 accttattac tcggtaagca gtttgggggc aaggtggaat gcagcagtaa aacgtgctgg
     5701 tattcgccgc cgtaatccgt accatacgcg gcatactttt gcctgctggc tgttgacggc
     5761 aggagcgaac ccggcattta tagccagcca gatggggcat gaaactgcgc agatggtgta
     5821 tgaaatttac ggtatgtgga ttgatgatat gaacgacgaa caggtagcca tgttgaatgc
     5881 gcggctatcg tagttgcaaa gtttgccccc aatttgcccc atttagtacc agagaaccga
     5941 aataatgcaa gaaaatcaaa agaatacgaa gaaagaacaa tacaacctga acaaattaca
     6001 aaaacgcctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6061 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6121 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6181 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6241 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6301 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6361 actgggggcg acgaagatcg cgttgggcca ccatcgtgac gatatcctgc aaacgttgtt
     6421 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6481 tggcaaacat atcgttattc gtccgctggc ctactgccgc gagaaagata ttcagcgatt
     6541 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     6601 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     6661 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     6721 gttcgatttc aaaggcatca cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     6781 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     6841 gttggatgag ttacggctga atgtggttga agtgaaataa ctcggatagc gcccgatgcg
     6901 caaacgtatc gggctactct aatggaggct gaataagacg cggtcagcgt cgcatccgac
     6961 aatcccgacg aagatgttta cttttgcatc aggcaattca acattttatt attttaataa
     7021 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7081 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7141 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7201 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7261 ggaactatta gctggtggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7321 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7381 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7441 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcgaccag
     7501 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcacgatcgc gttgctccag
     7561 atcgccgtgt aatgacaatg cactttgccc tacttcattc agcgcgtcgc agacagcctg
     7621 gcaatctttt ttggtattgc aaaacaccac gcaagaggat ggctgatgca agcttaataa
     7681 ccgttgcaac agaggaattt tgcctttgct ggatgtctca taaaattgtt gttcaatggg
     7741 tggcaaagca tctgttgagt caatttcaat cgccaaagga tcgcgttgca ctcgtccgct
     7801 gattgcggcg atggcttccg gccaggttgc cgaaaacaga agcgtctgtc gagatgcagg
     7861 cgcaaaacgg atgacatcat caatggcatc gctaaatccc atatccagca tgcggtcggc
     7921 ctcatccatc accaaagtat tcagcgcatc cagtgatacc gtgccttttt gcaggtgatc
     7981 cagcaaacgg cccggcgttg ccacgataat atgcggcgca tgttgcaacg aatcacgctg
     8041 cataccgaac ggttgaccac cgcacaacgt caaaattttg gtatttggca gaaaacgcgc
     8101 cagccgacgc aattcacctg ccacctgatc cgccagttca cgcgtaggac acagcactaa
     8161 agcctgggtt tgaaatagcg acgcatcaat ttgctgtaac aagccgaggc caaaagccgc
     8221 cgttttgccg ctgccagttt tcgcctgcac gcgaacatct tttccggcaa ggatcgccgg
     8281 aagcgcggcg gcctgcaccg gcgtcatggt taaataaccc aactcattaa ggttcgtgag
     8341 ttgggcggga ggcaaaacat tcagggtaga aaaagcggtc acaatctatt ctcgtggtca
     8401 tcgacgcaaa gtcatcaggc gcgtatcctc gcagatctac gctcacgatg cgacaattta
     8461 atcggttctt catcgggtgg tgggtcaggc atgggttgcg ggcgagggat cggatcgggc
     8521 actggaacag gatcgccagg aatcggttca gggacaggaa tttgcaaata aataagtgtc
     8581 gtcatatttc cctccggtca ttgggtggac tcttaaaggg tagacgctga taaataacag
     8641 gcaaaaaaaa gccgactcat caaagtcggc gtcgtacgaa tcaattgtgc tatgcagtaa
     8701 ttcaaaaaag gaagtaagac aatatggagc gcaacgccca tcgcttgacg ttgcattcac
     8761 ctgcaagaga gatattgccc tgaatgggta gagagtttat tgacttcgct caaactttgc
     8821 ggcgtttttg tatacagaca gccggaaaaa ttgcttttgt tacaaccatt tactacgatg
     8881 caaccataaa gcaacaccac caataagaac aactaacaga atacaaaaaa ttgaaaatcc
     8941 gaattgccac ccgccgccag ggatcccacc aaggttgacg ccaaataacc ctgtcagaaa
     9001 ggtactgggt aaaaagacca ttgccatcaa tgacattgta taggtacgac gagctaaatt
     9061 ttcctgcatc acctgagcga tttcatccgc catcacacca gtccgtgcta tacaggcgtc
     9121 gatttcgtca aggccgcgcc caaggcgatc ggcaatatcc tgcatccgac ggcgttggtc
     9181 atcgctcatc cacggcaaac gttcactggc aagacgagca taaacatcac gttgcggtgc
     9241 catatagcga cgcatcacaa ttaattgttt gcgcagcaga gccaggaatc cacgcggcgg
     9301 aatttgctga tcaaggagat tatcttcaag gtcgataatt ttatcgtgca gctgctcgat
     9361 aaattcgctg gaatgatcgg tcaacgcatc gcacacatcc accagccatc ccccgcaatc
     9421 ggtcggaccc gtgccctctt ccagatcact caccacatcg tccagcgcca gcactttgcg
     9481 ttgtcgggtc gaaacaatta accgcccgtc catatataca cgcatggcga ccagttgatc
     9541 ggggcgttca tcggtgctgc cgtttataca gcgcaatgta atcagcgtgc cttcaccgag
     9601 acggctgact cggggccgtg tgctctcgcc cgccagcgca tcacgtacgt tattgggaag
     9661 cagcggtgtt gtcgccagcc attgggcgct atcatggtgt acataattaa ggtggagcca
     9721 gcagggatgc gcttcatcaa tcacatctgt attttccagc ggtttaacgc cgcctctacc
     9781 atccagcatc caggcaaata ctgcatccgg gacattaacg tccgatccct taatcgcttc
     9841 cacagtgcct ccatcatcaa cgcattattt tgtagtctag ccttctggcc ctgttacgca
     9901 acatctcatc accccattac cctgaaatga ttaataaaat tctgtctaaa ttgaatacaa
     9961 aaagcaaaat gcttttccgt atacaaaccg tgtgaagtgt taaatagcgt ctatcattat
    10021 cagaattatc tgatcatatg acgtggcttt tttgcgatcg gatagcaaca aaaattgata
    10081 aaaataatgg gatctcaatg attacgcaca acttcaatac tctggactta ctcaccagtc
    10141 ctgtctggat cgtttcgccc tttgaggaac agttaattta tgccaacagc gcggcgcgac
    10201 tgttgatgca agacctcacg tttagtcagc tacgaaccgg tccctattcc gtctcctcac
    10261 aaaaagaact gccgaaatac ctctccgatc tgcaaaacca acacgatatt atcgaaatcc
    10321 tcactgttca gcgtaaagaa gaggaaacag cattaagctg tcggcttgtt ttgcgagagc
    10381 tgacagaaac agaaccggtg attattttcg aaggtatcga agcgccggca acgctgggtt
    10441 taaaagccag tcgctcggca aattatcagc gcaaaaaaca aggtttttat gcgcgctttt
    10501 ttctgactaa ctctgcacca atgttgttga ttgacccgtc acgagatgga caaatcgtcg
    10561 atgctaacct cgccgcgctc aatttctatg gttataacca tgaaacgatg tgccagaaac
    10621 atacctggga aataaatatg ctcgggcgtc gcgtcatgcc tatcatgcat gaaatctcgc
    10681 atttacccgg tggtcataaa cctctgaatt ttattcataa gctggcggat ggttcgactc
    10741 gtcatgtgca gacctatgcc ggaccgattg aaatttatgg cgacaagctc atgttatgta
    10801 ttgtgcatga tattactgag caaaaacggc tggaggagca gctggaacat gctgctcacc
    10861 atgacgcgat gaccggatta ctgaatcggc gacagtttta tcacattacg gaaccaggcc
    10921 aaatgcagca cctcgccatc gctcaggatt acagcttatt gctcatcgac acagatcgtt
    10981 ttaaacacat taacgatctc tatgggcatt ctaaaggtga tgaggtgtta tgcgccctcg
    11041 cccgcactct cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag
    11101 agtttgtctt attactacca agaaccccac tggataccgc gctttcgctg gctgaaacta
    11161 tccgcgtaag cgtggcaaaa gtgagtattt cgggcttacc acgctttacc gtcagcattg
    11221 gtgtggcgca tcacgaagga aatgaaagca tcgatgaact gtttaaacgc gttgatgatg
    11281 ctttgtatcg ggcgaaaaat gatggacgca accgcgtgct ggcggcataa gccacggatg
    11341 cgtctcgaga tcaacgactg cgcttagcgt ggcgctccca gttttcttgc tttgcctgtg
    11401 ccgttttacg tagtgcgacg taacacgccc cgctgccgcc atgatgcggt agcgcggtac
    11461 aatatgcctg gacatcatca aattcggtca accagcgcgc cacatagctg cggacaatat
    11521 tggcatgcga tttatcatcc cgccctttac catgaataat cagcacgtta cgcaaaccat
    11581 ccgccatggc ttgttgaatg aaactgaaca tcattttgcg gcactcttcc accggctggc
    11641 gcaaaagatt caggctcgcc tgttgcggat atttaccact gcgcagctta tccagcaccc
    11701 catgttgcaa cccttcccgc cgaaactcca gcggctgact tagcgggatg atgtcgagaa
    11761 atccggtggt gaggaaatta tcaagttgca gcgtgtcgat acgctgcggc gcacgttggt
    11821 tacgcgttgg atgccagtgg acatcggtag cacgtttcag cggctggaca tcttccatgg
    11881 cgtcaagaaa cagcgatttg tcgtcaaggt tcatgttaca tcctcccgca attaagagcg
    11941 cgatatgata accagaccgg gtcggtccaa caacgtatta cccaaaattc cagtaataag
    12001 ttccaaatat tgccgatatt ttaagcaaaa tgcttatgca tgattattca ttcacgatat
    12061 taataatgta acttatattt tcgtgaaatc tgtcactgaa gaaaattggc aactaaaggt
    12121 taaaaccgtt ataacacagt caccggcgca gaggagacaa tgccggattt aagacgcgga
    12181 tgcactgctg tgtgtactgt agagtctggc ggatgtcgac agactctatt tttttatgca
    12241 gttttaactt tgcagatagc cgcattctcg ccggaattgc gtgattaatg acgcggtcag
    12301 tggtgtctgg cgactatcac gccgctgaat caaataatag gccgctttcg gtaaaatttc
    12361 gctaaccggc aacatcacca gcccctgtcc gtgcaaggga tcgcagccca tttcttcagg
    12421 cagtatgctg agaaaatcgc tttttgccac cagactgata caggctgaga acgtctcgca
    12481 gactacaccg acctgtggcg tttgcgcctg atcgtcaagc aattcactca actgtttgta
    12541 gtagctgccg tgtggcgtcg gcattgtcca gctgtaatcc agtaactgtt tgatcgaacg
    12601 ggcaccaatg gcggggtgtc ccgggcggca aaagatcgcg aattgctttt ccagtaattt
    12661 ctcaaaagta aattcgtggt cgtacggtcc ctgataatag gtattgatgg tgaaatccaa
    12721 ttctccctga cgcaattcat taatcatcga caccagttgc ccttccataa tgcgtacttt
    12781 tacctgcgga tgctgctgat gaaaacgaga tattacagct ggcatcagac tgcgggaaat
    12841 actggccccc atgccgatat taatctgccc tgccagttgc ccttgtcgtt ggcgaatatc
    12901 ctcttgggct gcgcgcagtt cttcaagaat aagactggcg tgctgataaa aactttcacc
    12961 ggcatcagtt aacgtcacgc ctttactacg gcgaaaaaag agttgcgccg ctaacccttc
    13021 ttccagctcc tgaatagatt tactcagtgc cggttgcgac atattcaaca ttcggctcgc
    13081 tccgcgaatg ctgccctgac gagccacttc aacaaaagcc cgaatttgat gaatttttac
    13141 ctgaaaagcc atgacgccac cgataaccgt tatttatcag accaaagaaa ctggcatcta
    13201 ctttaatgca gatgattgtg tcagggtaat ttatgaacgg ttaaaactgt gaaaaatcag
    13261 ttagtgataa gtaaaaacta tcgctacgtg aaccgggtca cactttttac tgatgacggg
    13321 aaaggttatg gagtctttga atcaatttgt taattcgctt gccccaaaat tatcgcactg
    13381 gcgacgtgat tttcatcact atgcagagtc tggctgggtg gaattccgca ctgccaccct
    13441 tgttgcggaa gaattgcagc agctcggcta ctcactggcg ctgggccgcg aagtagttaa
    13501 tgaaaatagc cggatgggac tacctgatga actcactcta caacgcgaat tcgagcgcgc
    13561 tcgtcaacag ggtgcgctag cacaatggat tgcggctttt gaaggtggtt tcacgggtat
    13621 cgtcgccacc ctggataccg gtcgccccgg tccggtgatg gctttccgtg tcgatatgga
    13681 cgcgctggat ctcagtgaag agcaggatgt cagccatcgc ccctaccgcg acggttttgc
    13741 gtcatgtaac gccggaatga tgcatgcctg tggtcatgat gggcatacca ccattgggct
    13801 tgggctggcg cataccctta aacaattcga gtccggacta catggcgtca tcaaactgat
    13861 ttttcagcct gcagaggaag gtacgcgtgg cgcgcgggcg atggtcgatg caggtgtcgt
    13921 agatgatgtt gattatttta ctgccgtgca cattggcact ggcgtacctg cgggcaccgt
    13981 ggtgtgcggc agtgataatt ttatggcaac caccaaattt gacgcgcact tcaccggtac
    14041 cgccgctcac gcaggcgcaa aaccagaaga cggttacaat gccttgttgg cggcagcaca
    14101 agccactctt gcactgcatg caatcgcccc gcacagcgaa ggagcttcca gagtaaacgt
    14161 gggcgttatg caggcaggaa gcggtcgtaa cgttgttcct gcctcggcgt tgctgaaagt
    14221 ggaaacacgc ggggccagcg acgtcattaa tcaatatgtt tttgaccgtg ctcagcaagc
    14281 gattcagggc gcagcaacca tgtatggtgt cggcgttgaa actcgtctga tgggtgcagc
    14341 taccgccagt tctccttcgc cgcaatgggt cgcatggttg caaagtcagg cggctcaggt
    14401 cgcgggggtc aatcaggcca ttgaacgtgt tgaagcgcct gcgggttccg aagatgccac
    14461 attaatgatg gcccgcgtgc agcaacatca agggcaagcc tcctacgtgg tgtttggcac
    14521 acagctggcg gcaggtcatc acaacgaaaa attcgatttt gacgagcagg ttctcgctat
    14581 tgccgtcgaa acgctggcgc gcaccgcgct caattttccc tggacgcgag gtatctgatg
    14641 caggaaatct atcgttttat cgacgatgcg attgaagccg atcgccaacg ttataccgat
    14701 attgccgatc aaatctggga tcatccagaa acacgttttg aagagttctg gtcagcggag
    14761 catctggctt cggcgctgga atctgcaggc ttcaccgtta cccgcaacgt aggcaatatc
    14821 ccaaatgcct ttattgcttc gtttggtcaa ggcaaaccgg ttatcgccct gctgggagaa
    14881 tatgacgccc tggcaggttt aagtcagcaa gcaggttgcg cgcaacctac atccgtgacg
    14941 cccggtgaaa atggtcacgg ttgcggacac aatttgctgg gaaccgccgc ctttgccgct
    15001 gcaatagcca tcaagaaatg gctggaacaa tatgggcaag gcggcacagt gcgcttttat
    15061 ggttgtcctg gcgaagaagg cggctcgggt aaaacgttca tggtccgcga gggggtattt
    15121 gatgatgtgg atgcggcact cacctggcac ccggaagcct ttgccggtat gttcaatacc
    15181 cgtacgctgg caaacattca ggcatcatgg cgctttaaag ggatcgcagc acatgccgcg
    15241 aattcccctc atttgggacg cagcgccctt gatgccgtaa cgttgatgac cactggcacc
    15301 aacttcctca acgaacatat tattgaaaaa gcgcgcgtac actatgccat cacaaatagt
    15361 ggcgggatct cgcccaacgt ggtccaggcg caggcagaag tgctttatct tatccgcgcc
    15421 cccgaaatga ccgacgtgca gcatatttat gatcgggtcg ccaaaatcgc cgaaggtgcg
    15481 gcattgatga ccgaaaccac ggttgaatgc cgcttcgaca aagcctgttc cagttatctc
    15541 ccgaatcgca ccttagaaaa tgccatgtac caggccctat cccattttgg taccccggaa
    15601 tggaactccg aagaactggc ttttgcgaaa caaattcagg ctacgcttac ctccaacgat
    15661 cggcaaaaca gtctgaataa tatcgccgca accggtggcg aaaacggcaa ggtttttgca
    15721 ctacgtcatc gtgaaacggt actggcgaat gaagtcgctc catatgccgc caccgataac
    15781 gtgcttgcgg catcgactga tgtcggcgac gtcagttgga aactgcctgt tgcccagtgt
    15841 ttcagcccct gttttgccgt cggtacaccc ctacatacgt ggcaactggt tagccagggg
    15901 cgaacatcta ttgctcataa aggaatgctg ctggcggcga aaactatggc agcaaccaca
    15961 ctcaatctct tcattgattc agggctattg caagaatgcc aacaagagca tcagcaagtt
    16021 acggacacgc aaccgtatca ctgccctatc ccgaaaaacg tgacaccgtc acctttaaaa
    16081 taacaacaac aacgcaaaca caacaaccga ggaatgccca tgagtatgtc atccataccg
    16141 tcgtcctccc aatccgggaa gctctatggc tgggtcgaaa gaattggtaa caaggttccc
    16201 catccttttt tgctctttat ctatttgatt atcgtactca tggtgacgac ggcaattttg
    16261 tcggcctttg gcgtcagtgc gaaaaacccg accgatggta cgccggtggt ggtgaaaaac
    16321 ctgctcagtg tggaaggatt acactggttt ttacccaatg ttattaaaaa ctttagcggt
    16381 tttgctccac ttggtgcgat cctggcgctg gttttaggtg ccggtctggc ggagcgcgtc
    16441 ggcttactgc cagcactaat ggttaaaatg gcatcgcatg ttaatgcccg ctacgccagt
    16501 tatatggtgc tgtttattgc ttttttcagc cacatttctt ccgatgcggc gttagtgatc
    16561 atgccaccga tgggtgcgct gatttttctg gcggtgggca ggcatccagt tgcaggttta
    16621 ctggctgcca ttgcaggcgt aggttgcggc tttacggcta atttactgat tgtcacaacc
    16681 gacgtgttgc tgtcggggat cagcacggaa gcggcagctg cgttcaatcc gcaaatgcac
    16741 gtcagtgtaa ttgataactg gtattttatg gccagctccg tagtcgtact gacgattgtt
    16801 ggcggcctga taaccgacaa aatcatcgag ccacggttag gtcaatggca gggaaacagc
    16861 gatgagaaac tgcagacatt gaccg
//
LOCUS       DAZFTN010000098        16709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFTN010000098 DAZFTN010000000
VERSION     DAZFTN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(144..2093)
                     /locus_tag="ACHKV2_003685"
     CDS             complement(144..2093)
                     /locus_tag="ACHKV2_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFO6941494.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVRDALRRKRGKQTG"
     gene            complement(2184..2268)
                     /locus_tag="ACHKV2_003686"
     tRNA            complement(2184..2268)
                     /locus_tag="ACHKV2_003686"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Leu,seq:caa)
     gene            2464..3483
                     /gene="ahr"
                     /locus_tag="ACHKV2_003687"
     CDS             2464..3483
                     /gene="ahr"
                     /locus_tag="ACHKV2_003687"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO6941495.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3613..5115
                     /gene="yjgR"
                     /locus_tag="ACHKV2_003688"
     CDS             3613..5115
                     /gene="yjgR"
                     /locus_tag="ACHKV2_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO6941496.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5276..6358)
                     /gene="lptG"
                     /locus_tag="ACHKV2_003689"
     CDS             complement(5276..6358)
                     /gene="lptG"
                     /locus_tag="ACHKV2_003689"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6941497.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6358..7458)
                     /gene="lptF"
                     /locus_tag="ACHKV2_003690"
     CDS             complement(6358..7458)
                     /gene="lptF"
                     /locus_tag="ACHKV2_003690"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6941498.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7521..7571)
                     /gene="ytgA"
                     /locus_tag="ACHKV2_003691"
     CDS             complement(7521..7571)
                     /gene="ytgA"
                     /locus_tag="ACHKV2_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO6941499.1"
                     /translation="MSIIILATISTTQENK"
     gene            7725..9236
                     /gene="pepA"
                     /locus_tag="ACHKV2_003692"
     CDS             7725..9236
                     /gene="pepA"
                     /locus_tag="ACHKV2_003692"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO6941500.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9590..10033
                     /gene="holC"
                     /locus_tag="ACHKV2_003693"
     CDS             9590..10033
                     /gene="holC"
                     /locus_tag="ACHKV2_003693"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO6941501.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10033..12888
                     /gene="valS"
                     /locus_tag="ACHKV2_003694"
     CDS             10033..12888
                     /gene="valS"
                     /locus_tag="ACHKV2_003694"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO6941502.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQKSFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12943..14139)
                     /gene="yjgN"
                     /locus_tag="ACHKV2_003695"
     CDS             complement(12943..14139)
                     /gene="yjgN"
                     /locus_tag="ACHKV2_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO6941503.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            14332..14835
                     /gene="yjgM"
                     /locus_tag="ACHKV2_003696"
     CDS             14332..14835
                     /gene="yjgM"
                     /locus_tag="ACHKV2_003696"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6941504.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14881..15297)
                     /gene="rraB"
                     /locus_tag="ACHKV2_003697"
     CDS             complement(14881..15297)
                     /gene="rraB"
                     /locus_tag="ACHKV2_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO6941505.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            15459..16463
                     /gene="argF"
                     /locus_tag="ACHKV2_003698"
     CDS             15459..16463
                     /gene="argF"
                     /locus_tag="ACHKV2_003698"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO6941506.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(16520..>16709)
                     /locus_tag="ACHKV2_003699"
     CDS             complement(16520..>16709)
                     /locus_tag="ACHKV2_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941507.1"
                     /translation="DVIEEKLNNELKLRFRMMQTILKSEVNVSPFIDQQRLNTLNPPE
                     NLRIAIEKFGWKKKTITA"
BASE COUNT         4337 a         4211 c         4249 g         3912 t
ORIGIN      
        1 gagctaatgc aaatcaagtc tcaccagacg cattttaagc gtctccaaat ttggcactat
       61 taaatccacg acctttaata aaaaatgcgc caggaagcgt cccagcgcat tcaagagcat
      121 aactcaactg aatagtaact gactcaacct gtttgtttgc cacgctttct tcgcagcgca
      181 tctcttacag catccagccc atcaggaatg ttgaatctcg ctatctcggg caaagaaaaa
      241 tcaatctttt cgacaacctc aaccatgtga accggtttta aatctttgct gtatcgcact
      301 tcagtttctg ttttgcctcg cacatgccca agtagctgcc cacgcagttc cgaactaaca
      361 cccttctctt taagcaaagt tgaaacagaa tgccggaaag agtggaaaga tttgcttctg
      421 tctctttcaa gttttaatcg acccgcaagc aatgattcat tgaaccatgc ggaaaccggc
      481 ctaccatagc ctttggtttt atgtggttta agctcgggaa aaagacggtc atacccggca
      541 gctttcaacg catccacata ctcgataaaa ccgagttcaa tcagcgtaga atgaaccgga
      601 atcttgcggc gggaattggc gttttttagt ttctttccgt tttcgctatc gctttcaagg
      661 ttcaggtaat aaacgccgtc atctgccaga acatcgtcca gcattaactg tgcaatctcg
      721 ttaacccttg cccccgtcat caagcccaac aacggcgccc aatagtgaaa cgggcaatat
      781 tgatggaatc gcccttgcgc attacgttca acagttccag cgacaaacca cggttgcgaa
      841 aaaattgcct taatttcatg cggttcaaat atgtcagtgt gatcctgctc tcgctcacct
      901 ttgtcatcgg gagggaaaaa gttgtcgcat ggcgatttca gaaacttacc atcatcaaca
      961 gcccactcca ttgcaccata aaggccgttg atatactttt tgacagactc ttctgtcatc
     1021 agtttatcgc cacattctgc cgctttggcg attaactcat ccagcgtctt aaccccgtgc
     1081 ttacctttca tcaaattacg gttagcagga attgtgcgca acttgctctc ataagcccgg
     1141 aggaaatcac ggtcgaaatc ttccaactga atatccccca tcacttcaat catcaaatcg
     1201 aagtaatagc gaatatcgcg gagcgccgct tctgatacct tgcccttacg tcgatgtttc
     1261 aaatactctt cacgaagcca tgacaagttt tcattgtaat gtttgcggtg aacatattca
     1321 ttagcgataa caggagcgac aagaggcacg gcagggtgaa tgctaactgc tggtggcgtg
     1381 gtcttaacca gccatgtaag tcgaaggcct tcagcatgaa ctttgctaat caacaggcta
     1441 tcagcagtta tatctacgcc gggtaaatcg aagaaccaac ctccactttg gttatcacca
     1501 ctagttcgaa atgcagcctc tccttcggat actccagcgc tcaacagaca atctaaagta
     1561 tgccggggat tgaaggggcg gagataacca tttgcgaagc tctcgatacc cagctcaaac
     1621 gcgctattga gaatgatgac ttctttaggt tgatcctttt catcatcaga ctgtttttct
     1681 cgttttgcac ccggttcccg ctctactgca ttaaattcat caacatagaa accgaactga
     1741 ccagtcgcca gccagaatac cgggaggtta cgattaacca cctctcgaat caattgcgat
     1801 ataggcaact cgattgattg agcaaagtta gaaagcgaga tcataccctc accagcaagc
     1861 agtggggcgc agtcacttaa agttcgataa tccaattgtt catactcttg taaggtctga
     1921 taccagaccg ccaacaggag cgcggacttc tgctttgctt catgaagatc gtgagtgcct
     1981 gttgaagtgt gaatctcaca ctgtcctgcg tatcgacgaa aacgttgggg aacgctaatg
     2041 cggacaacat aaatgccgct ggttcgacga tagagtttat atcgctgaga catcgcctcg
     2101 tatacccttt tgtaagaggg gctacgcgat ttcaagcaag attatgaatt aaattgtttt
     2161 aaaacaatag tttagatgag tcttggtgcc gaaggccgga ctcgaaccgg cacgtatttc
     2221 tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac tgaagaggta
     2281 tgcggaaaac gttgtggatt atacctgtcg cacgccacca tgcaagcgcc agcttgctcg
     2341 cacctcgcta agtgctgaaa ttttcagcat tgcatacagc gatgtgtaac ctttgtcaca
     2401 ctccaggcac cccgccctgc catgctctac acttcccaaa caacaccaga gaaggaccaa
     2461 aaaatgtcga tgataaaaag ctatgccgca aaagaagcgg gcggcgaact ggaagtttat
     2521 gagtacgatc ccggtgagct gaggccacaa gatgttgaag tgcaggtgga ttattgcggg
     2581 atctgccatt ccgatctatc gatgatcgac aatgaatggg gattttcaca atatccgctg
     2641 gttgccgggc atgaggtgat tgggcgcgtg gtggcgctcg ggagcgccgc gcaggataaa
     2701 ggtttgcagg tcggccagcg tgtcgggatt ggctggacgg cgcgtagctg tggtcactgc
     2761 gacgcctgta ttagcggtaa tcagatcaac tgcgagcaag gtgctgtgcc gacgattatg
     2821 aatcgcggtg gctttgccga gaagttgcgt gcggactggc aatgggtgat tccactgcca
     2881 gaaaatattg atatcgagtc cgccgggccg ctgttgtgcg gcggtatcac ggtctttaaa
     2941 ccactgttga tgcaccatat cactgctacc agccgcgttg gggtaattgg tattggcggg
     3001 ctggggcata tcgctataaa acttctgcac gcaatgggat gcgaggtgac ggcctttagt
     3061 tctaatccgg cgaaagagca ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc
     3121 cgcgatccgc aggcactgaa agccctgtcg gggcagtttg atctcattat caatactgtg
     3181 aacgtcagcc tcgactggca gccttatttt gaggcgctga cctatggcgg taatttccat
     3241 acggtcggtg cggttctcac gccgttgccg gttccggcct ttacgctgat tgcgggcgat
     3301 cgcagtgtct ctggctctgc taccggtaca ccttatgagt tgcgtaagct gatgcgcttt
     3361 gccgcccgca gcaaggttgc gccgactacc gaattgttcc cgatgtcgaa aattaacgat
     3421 gccatcaagc atgtgcgcga cggtaaggcg cgttaccgcg tggttctgaa agctgacttc
     3481 tgatctcttt gccgggtgcc tgtcgcccgg cacttaaatc tctttggaag gcgtctcgca
     3541 atctgctgaa tttagcggtg aagagtgcgc agccgttgcc tatactccag gcaggaaact
     3601 ggaggatatc tcatgagcga acccctgtta attgcccgca cgccggacac agaactattt
     3661 ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa
     3721 actgtcacgc tgcaaaaact ggcggagtca ttgtcggaaa tcggcgtgcc ggtgtttatg
     3781 gctgatgtga aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc ggaaaaactg
     3841 ctcgcaaggc ttaaaaatat cgacgtcaat gactggcaac cgcatgctaa tccggtggtg
     3901 gtgtgggata tctttggcga gaaaggccat tcggtacggg cgacggtttc agacctgggg
     3961 ccactgttgc tggcgcggct gttgaatctc aacgatgtgc aatctggcgt gctgaatatc
     4021 atcttccgca ttgctgacga tcaggggctg ttactgctcg actttaaaga tctgcgggcg
     4081 attacccagt acatcggcga taacgccaaa tctttccaga atcagtacgg aaatatcagt
     4141 agcgcatcgg ttggtgccat ccagcgcggg ctgttgtcgc tggaacagca aggcgcagca
     4201 cacttctttg gtgagccgat gctggatatc aaagactgga tgcgcaccga tgccaacggt
     4261 aaaggcgtta tcaatatcct cagcgccgag aaactttatc agatgccgaa actgtacgcc
     4321 gccagcctgc tgtggatgct ctcagagttg tatgaacaat tgccggaagc gggcgatctg
     4381 gagaaaccaa aactggtgtt tttcttcgac gaagcacatc tgctgtttaa cgatgcaccg
     4441 caggtactgc tggataagat tgagcaggtg ataaggctta ttcgctcaaa aggcgtgggc
     4501 gtctggttcg tttcgcaaaa cccgtctgat attccggata atgtgctcgg ccagctcggt
     4561 aatcgcgttc agcacgcttt gcgggctttt acgcccaaag atcagaaagc agtaaaagct
     4621 gcggcgcaaa ccatgcgggc caatccggcg tttgataccg aaaaggcgat tcaggaactg
     4681 ggcaccggcg aggcgttgat ctcttttctg gatgcgaaag gaagcccttc tgtggtggag
     4741 cgtgcgatgg tgatcgcgcc ttgttcgcgg atggggccgg tgacggaaga tgagcgtaat
     4801 ggcttgatta atcactctcc ggtgtatggc aaatatgagg atgaggtgga ccgggaatcc
     4861 gcctatgaga tgttgcaaaa aggctttcag gccagtaccg agcagcaaaa taatcccccc
     4921 gcgaaaggga aagaggtagc ggtggatgac ggcattcttg gtggattgaa ggatattttg
     4981 ttcggcacta ccggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggccaaa
     5041 agcgccgctc gccaagtgac gaatcagatt gtacgcggga tgttggggag tttgctgggg
     5101 gggagaagaa ggtaagtcag gcgagatttg tctttgcctg atgcgacgct ggcgcgtctt
     5161 atcatgccca ccccactgca atatattgaa ttttaattat ttttcaggaa tgaacgaagc
     5221 acactttgct aacaataata aagggagctt tcgctccctt tattcgttca ttcggttacg
     5281 attttctcat taacagccac aggctgatta agaagaagct ggcgcttggc aacagtgcgc
     5341 cgatgatcgg cgggatgcca taaaccaacg tcagcgggcc gaagatctgg tccagtacgt
     5401 agaagacaaa accgaaactg ataccggtga ccacacgcac gcccatcggt acgctacgca
     5461 gtgggccaaa gatgaacgac agcgccatca gcatcatcac cgccacggat agcggctgga
     5521 agattttgct ccacatgttg agctgataac gtccggcatc ctgaccgctc gacttcagat
     5581 acttcacata gttgtgcaaa ccgctgatag agagtgcatc cgggtccagc gccaccacgc
     5641 ccagtttgtc cggcgtgagg ttggttttcc aggtgccgct caccgtctgc gaaccggtaa
     5701 tctgtttcgg attggtcaga tcagattcat caacctgcga caaacgccag actttatgtt
     5761 ccgggtcaaa cttcgcagta gcggcatagc gtacggattg cagacgacga ttctcgttaa
     5821 aggcataaat gctgatgcca cctaactctt cgtcaccttt aacccgctca atgtagacga
     5881 agttgttgcc atctttcgcc cataagcctt gctgggtaga gagcaacgag ccgccgtaca
     5941 tcgcctgcgc acggtagtta cgcgccatct gctcgccctg cggcgcgacc cattcgccaa
     6001 tcgccatcgt cagcaagacc agcggaatgg cggttttcat caccgacagc gccacctgca
     6061 tacgggtaaa accagaagcc tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa
     6121 gaccaagcaa cgccccaagc agagccgcca tcgggaagaa tatttgcaca tctttcggca
     6181 cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta actcccctgc ccggcttttt
     6241 tcaactgatc gacaaacttg ataatgcccg acagcgacac cagcatgaac agcgtcatca
     6301 tgatggtggt gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa ggttgcatca
     6361 caccgctcct ttacgcgaaa aactggcgcg caggcggcgg accggcacgg tgtcccaaag
     6421 gttgagaaca atcgccaaag ccagataaat caggttaacg gtccacatcc acagcgtcgg
     6481 gtccagctta cctttaccgc cgttcgattt cagggaggtc tggatcagga agaaaagtag
     6541 atacagcagc atggctggca gcatcgacag tacgcgtccc tgacgtgggt taaccacgct
     6601 cagcggtacg accataagtg ccatcataaa cacggtgaat accaacgtga tacgccagtt
     6661 cagttctgcg cgagcacgat cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc
     6721 ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa
     6781 atccgtaatg cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc cctggttgag
     6841 agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac
     6901 agaaggacgt gcattacctt ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct
     6961 gccgtcaacg ctttcgatga acagcaccga actgccatta gtcgcttgct ggaattgccc
     7021 ttgcgccagc gccgccatgc cagggttcgc tttcgcttct gctaacactt catcctgatg
     7081 acgcgatgac cacggtcccg cccacatcac gttaaccgcc gcgacgattg ccgtgaatac
     7141 cgcaaggatc attgccgctt tcaccagaac cgctttgctc aggccgcagg catgcattac
     7201 cgtaatttca ctttcggtat acagtttgcc cagcgtcatc agcagcccga ggaacaggct
     7261 taatggcagg ataagctgcg ccatttccgg cacgcccaac ccgagaaggg agagcaccag
     7321 attcgccgga atatcgccgt caaccgctgc gccgaggatc ctcactaact tttgacagaa
     7381 gaagatcaaa agcaagatga agagtatcgc cagctggctt ttgagcgtct cccgcaccag
     7441 atatcttatg attatcactt taaatacgcc cgtaaaaact cgtcttttgc aggattttag
     7501 cttgtttcat ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt cgctaagata
     7561 attatactca acggattcac ctctcagatt ttgttctgac gtgccaatgc cgtaataacg
     7621 ttaagattaa cacgaagtca tcgcaacagc ggacatgagt tacgaaagct tgcaattcta
     7681 tctgtagcca ccgccgttgt ctttaagatt caggagcgta gtgcatggag tttagtgtaa
     7741 aaagcggtag cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac
     7801 gtcgcctttc tccgattgca gaacagctcg ataaaatcag cgatgggtac atcagcgccc
     7861 tgctacgtcg gggcgaactg gaaggaaaac cggggcagac attgttgctg caccatgttc
     7921 cgaatgtact ttccgagcga attctcctta ttggttgcgg caaagaacgt gagctggatg
     7981 agcgtcagta caagcaggtt attcagaaaa ccattaatac gctgaatgat actggctcaa
     8041 tggaagcggt ctgctttctg actgaactgc acgttaaagg ccgtaacaac tactggaaag
     8101 tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag tttcgatcag ctgaaaacga
     8161 acaagagcga accgcgtcgt ccgctgcgta agatggtgtt caacgtgccg acccgccgtg
     8221 aactgaccag cggtgagcgc gcgatccagc acggtctggc gattgccgcc gggattaaag
     8281 ccgcgaaaga tctcggtaat atgccgccga atatctgtaa cgccgcttac ctcgcttcac
     8341 aagcgcgcca gctggctgac agctacagca agaatgttat cacccgcgtt atcggcgaac
     8401 agcagatgaa agagctgggg atgcattcct atctggcggt cggtcagggt tcgcaaaacg
     8461 aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca cgcccaatcg
     8521 tgctggtggg taaaggttta accttcgact ccggcggtat ctcgatcaag ccttcagaag
     8581 gcatggatga gatgaagtac gatatgtgcg gtgcggcagc ggtttacggc gtgatgcgga
     8641 tggtcgcgga gctacaactg ccgattaacg ttatcggcgt gttggcaggc tgcgaaaaca
     8701 tgcctggcgg acgagcctat cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg
     8761 ttgaagtgct gaataccgac gctgaaggcc gcctggtact gtgcgacgtg ttaacttacg
     8821 ttgagcgttt tgagccggaa gcggtgattg acgtggcgac gctgaccggt gcctgcgtga
     8881 tcgcgctggg tcatcacatt accggtctga tggcgaacca taatccgctg gcccatgaac
     8941 tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt
     9001 atcaggaaca actggagtcc aattttgccg atatggcgaa cattggcggt cgtcctggtg
     9061 gggcgattac cgcaggttgc ttcctgtcac gctttacccg taagtacaac tgggcgcacc
     9121 tggatattgc aggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc ggtcgtccgg
     9181 tagcgttgct ggcacagttc ctgttgaacc gcgctgggtt taacggcgaa gagtaattgc
     9241 gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc ttatccgacc tacacagcac
     9301 tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca ggcaatgctg ttattgtcgg
     9361 atgcggcatg aacgccttat ccgacctaca cagcaccgaa ctcgtaggcc tgataagacg
     9421 cggcagcgtc gcatcaggca atgctgttat tgtcggatgc ggcgtgagcg ccttatccga
     9481 cctacacaca gcactgaact cgtaggcctg ataagacaca acagcgtcgc atcaggcgct
     9541 gcggtgtata cctgatgcgt atttaaatcc accacaagaa gccccatata tgaaaaacgc
     9601 gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact
     9661 ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtgctca tcgcctgtga
     9721 agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt
     9781 tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg
     9841 gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc
     9901 agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa
     9961 acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc
    10021 aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag
    10081 cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagaa aagtttctgc
    10141 atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa
    10201 accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag
    10261 gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca
    10321 gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa
    10381 tggaaagcgg aatccggcgg cacgattacc cgtcagatgc gccgtctcgg caactccgtc
    10441 gactgggaac gtgaacgctt cacgatggac gaaggcctgt ccaatgcggt gaaagaagtt
    10501 ttcgttcgtc tatataaaga agacctgatt taccgtggca aacgcctggt aaactgggac
    10561 ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg
    10621 atgtggcaca tccgctatcc gctggctgac ggtgcgaaaa ccgcagacgg taaagattat
    10681 ctggtggtcg cgactacccg tccggaaacc ctgctgggcg atactggcgt agccgttaac
    10741 ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac
    10801 cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg
    10861 aaaatcactc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca cgcgctgccg
    10921 atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt gttcgatacc
    10981 aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca gaaactcgag
    11041 cgttttgctg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa
    11101 attaaaccgc acgacctgac agttccttac ggcgaccgtg gcggcgtagt tatcgaacca
    11161 atgctgaccg accagtggta cgtacgtgcc gatgtactgg cgaagccggc agttgaagcg
    11221 gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg
    11281 atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca ccgtatcccg
    11341 gcatggtacg acgaagcggg taacgtttat gttggccgca acgaagaaga agtgcgcaag
    11401 gaaaataacc tcggtgccga cgttgccctg cgtcaggacg aagacgttct cgacacctgg
    11461 ttctcttctg cgctgtggac cttctctacc ctaggctggc cggaaaacac cgacgcactg
    11521 cgccagttcc acccaaccag cgtgatggta tctggtttcg acatcatctt cttctggatt
    11581 gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg
    11641 ccgttccaca ccgtttacat gaccggtctg attcgtgacg acgaaggcca gaagatgtcc
    11701 aaatccaagg gtaacgttat cgacccgctg gatatggttg acggtatttc gctgccagaa
    11761 ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa aatccgtaag
    11821 cgcaccgaga agcagttccc gaacggtatt gagccgcacg gcaccgatgc cctgcgcttc
    11881 accctggcgg cactggcgtc taccggtcgt gacatcaact gggatatgaa gcgtctggaa
    11941 ggctaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacacg
    12001 gaaggtcagg attgcggctt caacggcggc gaaatgacgc tgtcgctggc ggaccgctgg
    12061 attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc
    12121 ttcgatatcg ccgcaggcat tctgtatgag tttacctgga accagttctg tgactggtat
    12181 ctcgagctga ccaagccggt aatgaacggt ggcaccgaag cagaactgcg cggtactcgc
    12241 catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc
    12301 atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc
    12361 atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac
    12421 accgaatggc tgaaacaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc
    12481 gcgccgggca aaccgctgga gctgctgctg cgtggttgca gcgcggatgc agaacgtcgc
    12541 gtaaatgaaa accgtggctt cctgcaaacc ctggcgcgtc tggaaagcat caccgtgctg
    12601 cctgccgatg acaaaggtcc ggtttccgtt acgaagatca tcgacggcgc agagctgctg
    12661 atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg
    12721 gcgaagatcg aaggcgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc
    12781 gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg ctatgcggaa
    12841 gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc aaaacacagt
    12901 gatgaaaacg aaggccggag catgctccgg cctttttatc tcttacagaa atggtaaaga
    12961 aggcataatt ccccgtgaaa tactggctaa gaagccttta tctggttgtt cttctttatt
    13021 gattaaagga aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat
    13081 catccatatt ttcagcagtg gataagccag accgcccgta atcccggata tcaccaccaa
    13141 cgcacacatg cgataaagca taccgtggta tgttaaagtt gagcgaaaac gaatacgccc
    13201 atcattcagt gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt
    13261 gctaacagca atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc
    13321 cataaattgc tgtaaattct caatatcatc atttgcatac acagaactat catacgcatt
    13381 taatatttga tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa
    13441 tgcgaggatg gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat
    13501 accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa
    13561 aataccaatg gaaacaatac caactattgc catcagaacc acagatacaa taacactact
    13621 tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc
    13681 cattaaaatc ggcaagaaaa aggttaccca ccagacccct ttcatagagc aattaaaact
    13741 aaatcttacg ccgttgagac tggtcatcaa ggcctgataa cgcagtcctt ttgctgccat
    13801 aaatataagc aaaaccaaca gtgacaaagt caaaacacag ccaataagcg gcatatctgc
    13861 tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac
    13921 attcccacca gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcatagag
    13981 ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa
    14041 caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaacga
    14101 atgggacgga acatccattt cattaataac ttgagccata aaaattccct gaattaaaaa
    14161 atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct
    14221 tatcagtaat aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag
    14281 agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac
    14341 attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc
    14401 cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg ctacaccgtc
    14461 gccgatccga atcttgacga gctgtatcaa gtgtatagtc agccgggtca tgcatattgg
    14521 gtggttgagt acgagggtga agtggtcggc ggcggtggga ttgcgccatt agcaggtagt
    14581 gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg cggcaaaggg
    14641 ctggcaaaaa aactggcctt aaaggcgatg gaggaggcaa gagaaatggg cttcaaacgc
    14701 tgctatctgg aaacgaccgc ttttttgaaa gaagcgattg gtttgtatga acatttaggt
    14761 tttcaacata tcgactatgc acttggctgc acgggccatg ttgattgtga agtgcggatg
    14821 ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt aatcaattaa
    14881 ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc cgtcttcgcc
    14941 gttgggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag
    15001 cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag
    15061 gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac
    15121 ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca gatcgtctgc
    15181 ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc
    15241 ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc
    15301 tcattcctct taatgtcctg ccgatactca tattgtcaca caccactggc attgcctcca
    15361 cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca tataaattga
    15421 attttaattc attgagacgt tagccacagg agggatctat gtccgggttt tatcataagc
    15481 atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg
    15541 cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact ggtaaaaaca
    15601 tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa gttgccgcat
    15661 atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt ggtcataaag
    15721 agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt cagtatcgcg
    15781 gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg
    15841 gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc
    15901 atttacccgg taaaccattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca
    15961 acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg
    16021 cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgcc ctggcacaac
    16081 aaaatggcgg gaatattacg ctgacggaag atgtagctaa aggcgttgaa ggtgctgact
    16141 ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa agaaaaatgg gctgaacgca
    16201 ttgcattact gcgtgattat caggtgaaca gcaagatgat gcagttgacc ggtaacccgg
    16261 aggtcaaatt cctccactgc ctgcccgcgt tccatgatga ccaaacgacg cttggcaaga
    16321 aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag gtcttcgaat
    16381 ctgccgccag cattgttttt gatcaggcgg aaaaccgcat gcacaccatc aaagcggtga
    16441 tggtcgcgac actcagtaaa taattgatat cgctataaag atgccgggaa atacagaaac
    16501 atcccggcgt caaattattt tatgcagtga tagttttttt cttccagcca aatttttcta
    16561 ttgctatacg taaattttcc ggaggattta gtgtatttaa acgctgttgg tcaataaatg
    16621 gcgatacatt aacctccgat ttcaagatag tttgcatcat tctaaaccta agttttaatt
    16681 cattattaag tttttcttca ataacatct
//
LOCUS       DAZFTN010000099        16584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFTN010000099 DAZFTN010000000
VERSION     DAZFTN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1416
                     /locus_tag="ACHKV2_003700"
     CDS             <1..1416
                     /locus_tag="ACHKV2_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6941508.1"
                     /translation="TLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTD
                     KDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPV
                     KVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQ
                     QETEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMG
                     DATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVN
                     GHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETA
                     GGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGAN
                     FDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDA
                     RYQAKYVSVIWQKLIPNQADF"
     gene            1481..1729
                     /locus_tag="ACHKV2_003701"
     CDS             1481..1729
                     /locus_tag="ACHKV2_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6941509.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            1707..2837
                     /locus_tag="ACHKV2_003702"
     CDS             1707..2837
                     /locus_tag="ACHKV2_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6941510.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(2883..3521)
                     /gene="ompW"
                     /locus_tag="ACHKV2_003703"
     CDS             complement(2883..3521)
                     /gene="ompW"
                     /locus_tag="ACHKV2_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO6941511.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSVGYRF"
     gene            3761..3850
                     /locus_tag="ACHKV2_003704"
                     /pseudo
     CDS             3761..3850
                     /locus_tag="ACHKV2_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3878..4621
                     /gene="yciC"
                     /locus_tag="ACHKV2_003705"
     CDS             3878..4621
                     /gene="yciC"
                     /locus_tag="ACHKV2_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO6941512.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            4651..5190
                     /gene="yciB"
                     /locus_tag="ACHKV2_003706"
     CDS             4651..5190
                     /gene="yciB"
                     /locus_tag="ACHKV2_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO6941513.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            5295..5693
                     /gene="yciA"
                     /locus_tag="ACHKV2_003707"
     CDS             5295..5693
                     /gene="yciA"
                     /locus_tag="ACHKV2_003707"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO6941514.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(5733..6452)
                     /gene="tonB"
                     /locus_tag="ACHKV2_003708"
     CDS             complement(5733..6452)
                     /gene="tonB"
                     /locus_tag="ACHKV2_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO6941515.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            6676..6972
                     /gene="yciI"
                     /locus_tag="ACHKV2_003709"
     CDS             6676..6972
                     /gene="yciI"
                     /locus_tag="ACHKV2_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO6941516.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            7273..8526
                     /gene="kch"
                     /locus_tag="ACHKV2_003710"
     CDS             7273..8526
                     /gene="kch"
                     /locus_tag="ACHKV2_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO6941517.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(8581..8754)
                     /gene="yciY"
                     /locus_tag="ACHKV2_003711"
     CDS             complement(8581..8754)
                     /gene="yciY"
                     /locus_tag="ACHKV2_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO6941518.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            8897..10357
                     /gene="cls"
                     /locus_tag="ACHKV2_003712"
     CDS             8897..10357
                     /gene="cls"
                     /locus_tag="ACHKV2_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO6941519.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            10392..10721
                     /gene="yciU"
                     /locus_tag="ACHKV2_003713"
     CDS             10392..10721
                     /gene="yciU"
                     /locus_tag="ACHKV2_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO6941520.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10774..11778)
                     /gene="oppF"
                     /locus_tag="ACHKV2_003714"
     CDS             complement(10774..11778)
                     /gene="oppF"
                     /locus_tag="ACHKV2_003714"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO6941521.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(11775..12788)
                     /gene="oppD"
                     /locus_tag="ACHKV2_003715"
     CDS             complement(11775..12788)
                     /gene="oppD"
                     /locus_tag="ACHKV2_003715"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO6941522.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(12800..13708)
                     /gene="oppC"
                     /locus_tag="ACHKV2_003716"
     CDS             complement(12800..13708)
                     /gene="oppC"
                     /locus_tag="ACHKV2_003716"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO6941523.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(13723..14643)
                     /gene="oppB"
                     /locus_tag="ACHKV2_003717"
     CDS             complement(13723..14643)
                     /gene="oppB"
                     /locus_tag="ACHKV2_003717"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO6941524.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(14729..16360)
                     /gene="oppA"
                     /locus_tag="ACHKV2_003718"
     CDS             complement(14729..16360)
                     /gene="oppA"
                     /locus_tag="ACHKV2_003718"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO6941525.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         4299 a         4056 c         3969 g         4260 t
ORIGIN      
        1 actctctcgt cgccagtacc agaaactgat gcagtggaaa cggcaattcc ggacaacgaa
       61 aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc gtgaggggcc gttctacttc
      121 ctcttcaccg acaaggatgg cgaaaaatac ggtcgcgcaa acaaactttc tggtctggat
      181 aaggcgctgg ctgccggggc tactgaaatc acaaaagaag aatattttgc ccgaaaaaat
      241 ggcacataca caggcttacc gcaaaatgca aataccgcac aaaattctga acaaccagaa
      301 ccggtaaaag ttaccgctga cgaagtaaag aaaattatgc aggcagccaa tatcagccag
      361 cctgacgcca atcagttgct cgccgcatca cgtggtgaat ttgttgcagg gattagcgac
      421 ccgaatgatc cgaaatgggt gaaggggatt gaaacccgcg attctgtgaa ccagaaccag
      481 caagaaacgg aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac
      541 gagccagaaa cgaaacaacc tgagccagta gcgcaacagg aaccggaaaa agtctgcacc
      601 gcctgcggtc agaccggcgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca
      661 acataccagg aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag
      721 gaaatggaag gcgctgaaca tccacacaag gagaacactg acggcaatca gcatcacgat
      781 agcgataatg aaactggcga gacggcagat cactcaatta aggtgaacgg tcatcaagaa
      841 atcacatcca ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa
      901 aatcctgatg ccccgatcat ctcaataggt gcaatatttt tcgatccgca aaccggagat
      961 atgggaccgg aatttagtaa gactatcgat ctggaaactg ctggcggggt cattgatcgg
     1021 gacaccatta aatggtggct taagcaatca cgcgaagcgc aatctgccat tatgaccgat
     1081 gaaatcccgt tagatgatgc actattacaa ttgcgggaat ttatcgacga aaactccggt
     1141 gaattttttg ttcaggtctg gggaaatgga gccaacttcg acaacacgat tttgcgccgt
     1201 tcatacgaac ggcaggggat cccctgcccg tggcgttact acaacgatcg cgatgtacgc
     1261 acaatcgttg agctggggaa agccatagac ttcgatgcca gaacggctat tccattcgaa
     1321 ggtgagcgcc ataatgcact tgatgacgcc cgttaccagg caaaatacgt ttcagttatc
     1381 tggcaaaaac tgatcccgaa tcaggctgat ttttaatgtt caaccccggt cgttgcccac
     1441 cagctatagt ggcggcgacc atgattagcg aacgacgctc atggcaagac ttattctgct
     1501 cactgagtgg gcaaaagagg aattcagtga accggtccca actccgagta cgttaagtaa
     1561 atacgctaaa gccggaatga tatttcctct ccccaaaaaa gttggaagac gctggcgagt
     1621 ggatccgcaa gctcgctttg tcggaatggt aaacaagccg gaggtgatcg ccacagatca
     1681 ccctgctttg aagaggatac tggaagatgg cgcgcccgcg aaaatataaa accgatgttc
     1741 cgggattatc tccgtatttt gacaaaagaa ataacaaagt ttactggcgt tacaggcatc
     1801 ccataacagg caaaaatcac ggtctcggca gtattgacca gaaactggca gaaactattg
     1861 cagcagaagc gaacagccgt cttgcccggc agcaaatgga acaaatgctc agtctgcagg
     1921 agaaaattat tagtgatacc ggcggttcat caaccgttac catttttctg aataattaca
     1981 gaaaaattca acaggaaaga tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga
     2041 aagcggcccc tctcagggta tttgatgaac gttttggcac cagaccgtta gatgccataa
     2101 ccgtaaaaga tgtggtatca gtactggaag agtacaaggc cagaggacat aacagaatgg
     2161 gacaaatttt caggaaagta ctgatcgatg ttttccggga agctcagcaa acgggcgatg
     2221 tcccgccagg ctttaaccct gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac
     2281 agcgactgac ttttgatgag tggatgatga tttataacgc agcggaaaag gatggttact
     2341 ttttacagcg cggtatgctg ctggcactga tgacaggcca gcgcctttca gatatttgca
     2401 aaatgcaatt ttcggatatc cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa
     2461 cccggattgc catccctctg gctctgcgtt gcgataaatt aaatctcacc ctggatgatg
     2521 tggtgtcatc ctgccgcgat tgcgttctta gtccgtggct attgcaccac catcacgcga
     2581 aagggacagc taagcgcggc gggatggtta agccagcaac attaaccgtt gcatttaaaa
     2641 aagcccggga ttctgtggat tacaactggc gtgctaatgg cacccctccc tctttccatg
     2701 agcagagatc tttatcagag cgattgttca gagagcaggg ggttgatacc aaaattttgc
     2761 taggccattc gaatcaaaaa atgaccgata tttacaacga cgcacgcggt aaggaatgga
     2821 aaaaactggt catttgatga ccagttttgc agaggggttt tgcagaggtt ttgcagagaa
     2881 aattaaaaac gatatcctac ggagaacata aacacccacg gatccagtcg taccgagtct
     2941 tttacggtag taacaccaga tttgtactta gccgtggtat cgatatccat gtaccacact
     3001 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
     3061 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
     3121 ccattatcaa agaaggtggt gtagttaata cctgccccaa cataaggacg gaatttgctg
     3181 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatgatg aacggttgca
     3241 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
     3301 acaccaatgt tgtcggtcgc catataagta aacgtcaggc ccagttgagt gttattggtc
     3361 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
     3421 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagagaga
     3481 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
     3541 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
     3601 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
     3661 ctggttaacg tgatttaaat caatttttaa atattttcgc tacgcaaata ttaactcgtt
     3721 cgccacttgc cgcggtttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
     3781 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
     3841 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
     3901 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
     3961 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
     4021 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
     4081 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
     4141 aggattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
     4201 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgccaaa
     4261 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
     4321 cgttccggga attatcatgg ccatattact ggccctggca ccggtgatgc tggtacagga
     4381 caaaatgggc gtttttgcct cgatccgtag cagtatgcgg ctgacttggg cgaatatgcg
     4441 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
     4501 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
     4561 cctgatttca gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
     4621 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
     4681 gttgtctttt tcgcgtttta caaaatttat gacatctatg cggctactgc ggcgctgatc
     4741 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
     4801 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
     4861 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
     4921 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgggtaa agaactcacg
     4981 ctgccgcaac cggtatggtc gaagctgaat ctggcctggg ctgttttctt tatcctttgc
     5041 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
     5101 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
     5161 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtcctttgg ggctggcgct
     5221 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
     5281 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
     5341 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
     5401 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
     5461 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
     5521 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
     5581 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagac gcattattta
     5641 agtatgtcgc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
     5701 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
     5761 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
     5821 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
     5881 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
     5941 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
     6001 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ctgtacttga tgtcgggcgt
     6061 gccggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
     6121 ttttgctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
     6181 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
     6241 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
     6301 gcaaccatcg tgacagaaat cggctgcgca ggcgcaggta gttcaataac ctgatgtacc
     6361 gaggtataga gcagacccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
     6421 caggggaagc ggcgaggtaa atcaagggtc attgaagtca taatcatttc agtagaaaaa
     6481 ccaggtctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
     6541 cagtgaaaat gtcatttttg gcgtaattga ccttacttta tgcatggtct taacaaagac
     6601 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
     6661 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
     6721 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
     6781 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
     6841 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
     6901 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
     6961 aaagtgttct gagtagagat ccaatattta agggcacggt ttttgcaacc gtgttcttgt
     7021 ttggcctttc cttataggtc ggtcattgca ttcaccacca caagcagata ataactttat
     7081 ctgtatttct ttatatgatg tgagcatgtt caggaaaaat gccctttcct gacgcagagt
     7141 tgctcactca aaacctttga ttactgcgga taaagtcatc tactattgtt taacacattc
     7201 acgtgtcgca tgtatgttca gaaaatgtgc acgttaagat aattaaacaa aattgtggtt
     7261 acagaggtat cagtgagtca ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg
     7321 accctacggc acgacattct cgcgctggcc gtctttttaa atgggttgct tatttttaaa
     7381 acaatctatg gtatgtcggt caatttgctt gatattttcc atatcaaagc attttcagag
     7441 ttggatctct ccttgctggc aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg
     7501 aactccattg gcttactgtt ccgggcaaag ctcgcatggg caatcagtat cattttgttg
     7561 ttgatagcgc taatttacac cctgcatttt tatccctggc tgaaatttag tattggattt
     7621 tgcattttta cgctggtgtt tttactgata ctgcgcaaag acttctccca cagtagcgcc
     7681 gcagccggga caatttttgc atttattagt ttcacgacat tactgtttta ctcaacctac
     7741 ggtgcgcttt atttaagcga aggttttaat ccgcgaatag aaagtttgat gaccgcgttc
     7801 tatttttcga tagaaaccat gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa
     7861 tcagcacgat tgttcactat ttcggtcatt atttccggca ttaccgtttt tgccacatct
     7921 atgacctcaa tttttggtcc gcttatccgc gggggattca acaaacttgt aaaaggaaac
     7981 aatcatacaa tgcatcgtaa agatcatttt attgtttgcg gacattcgat tctcgccatt
     8041 aatacgatcc tgcaactgaa tcaacgcgga caaaacgtaa cggttatcag caacttgcct
     8101 gaagatgata tcaagcaact tgagcaacgc ttaggcgata acgctgatgt tatccccggt
     8161 gacagtaatg acagttctgt attaaagaaa gcgggaatcg atcgttgccg ggccattctg
     8221 gcgctgagtg ataacgatgc agataacgcg tttgttgtac tctcggcaaa agatatgagc
     8281 agtgatgtca aaacagttct cgccgtcagt gatagcaaaa acctgaataa gattaagatg
     8341 gtacatccgg atatcattct ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt
     8401 ttaaatggtg aagagattaa taatgatatg ctcgtttcaa tgttgttgaa ctcaggtcat
     8461 ggtattttca gcgataacga tgaacaagaa acgaaagctg acagtaaaga atcggcgcaa
     8521 aaatagagtt taaagacgga agagtaaact tattgggcac cgcaatggtg cccagtacat
     8581 ttagatattg tagatcgcaa acaataacga gttacgctgt ttgtttagaa tgcacttcct
     8641 gatgctgtgg atacgcatat ttcggcgcga ctgcccctca agccaacgcg atttacgtcg
     8701 gctagcctga cgctgcatgc gccagcgccc cacttccgtt ctactccgct tcatgttaac
     8761 tactctatgc aataacagaa tggtcattat acccccgccc ccgacgctga ccagtgcttt
     8821 tcccgcgttt ttatttgcca gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca
     8881 aaaggatttc taatctatga caaccgttta tacgttggtg agttggttgg ccattctggg
     8941 atactggttg ctcattgcag gcgtaacttt acgcattcta atgaaacgac gcgcagttcc
     9001 ctccgcgatg gcctggctgt tgattattta cattctgccg ttagtcggaa ttattgccta
     9061 tcttgccgtt ggcgagctcc atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg
     9121 gccttccacc gcaaaatggc ttaacgacct taaagcctgt aagcatatct ttgccgaaga
     9181 aaatagcagt gtcgctgcgc cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg
     9241 ggtcaaaggg aatcagctac aactgatgac cgagtcagat gatgtgatgc aggcgttaat
     9301 ccgcgacatc cagctcgcgc gccataatat tgagatggtg ttttatatct ggcagcccgg
     9361 cggcatggcg gaccaggtgg ctgaatcatt aatggcggct gcgcgacgcg gcattcattg
     9421 ccgattgatg ctcgactccg ccgggagtgt agcttttttc cacagcccgt ggcccgagct
     9481 aatgcgtaat gccggtattg aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt
     9541 tttacgccgt atggacctgc gccaacatcg caagatgatc atgatcgata attacatcgc
     9601 gtacaccggc agcatgaata tggtcgaccc tcgctacttc aaacaagatg cgggcgtagg
     9661 gcaatggatt gatctgatgg cgcgtatgga aggccccatc gccaccgcga tggggattat
     9721 ttattcctgc gactgggaga ttgaaacagg gaaacgtatt ctaccgccac cacctgatgt
     9781 caatattatg ccgtttgagc aggccagcgg tcataccatt cacacaattg cttctggccc
     9841 cggctttccg gaagatctca ttcaccaggc attattgact gcggcttatt cggcgcgtga
     9901 atatttgatc atgaccacgc cctactttgt gccaagcgat gatttacttc atgcgatttg
     9961 cacggcggcg cagcgcgggg tggatgtcag tattatcctt ccgcgaaaaa atgactcgat
    10021 gctggtcggc tgggccagtc gcgcattctt tacggaactg cttgctgctg gggttaaagt
    10081 ttatcagttt gaaggcgggt tactgcatac caagagcgtg ctggtcgatg gcgaactaag
    10141 tctggttggc accgttaacc ttgatatgcg tagtctgtgg ctgaatttcg agattaccct
    10201 ggcaatcgac gataaaggtt ttggtgcaga ccttgccgcc gttcaggacg attatatttc
    10261 gcgttcacgt ctgctcgatg cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc
    10321 cgagcgactg ttttacttct tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac
    10381 aggtagtgac tatggatatg gatctaaaca atcgcctgac tgaagatgaa acgcttgagc
    10441 aggcctacga tatttttctc gaactggctg ccgacaatct cgatccagct gatgttctgc
    10501 tgttcaatct tcagtttgaa gagcgcggcg gcgcggagtt attcgatccg gcggaagact
    10561 ggcaggaaca tgttgatttc gatttgaacc cagacttttt tgccgaagtt gtgattggtc
    10621 tggcggacag tgaagatggt gaaatcaacg atgttttcgc ccgcattctg ttatgccgcg
    10681 aaaaagatca caaactttgc catatcatct ggcgggaata aataatcact ttcggcgtga
    10741 aaaagggctg acagctgtca gccctttttg tgtttaaagc ggatcgactt tcaggcaaga
    10801 aacggcgtgt ctgaaactcc cctccagcac aggacgtgtt ttggcacact ccggaccggc
    10861 aatcgggcaa cgggtacgga aaacacaacc ggaaggcggg ttgatcggcg acggtaattc
    10921 cccttccagt aactggatgg ttttgttctt ctccagatcc ggatcaggta tggggactgc
    10981 cgacatcaat gccctggtgt aaggatgtag tggattgtgg tagacctcat cataggtccc
    11041 cagttctacc gcatggccga gatacatcac caacacacga tcggaaatgt gttttaccac
    11101 ggccaggtca tgagcgataa aaattaatga caatcccatc tcacgttgca attgctggag
    11161 caggttgacc acctgtgcct gaattgacac gtccagcgcc gacaccggct catcacagat
    11221 aatcagcttc ggttcaagaa taagagcacg cgcaatcccg atacgctggc actgcccacc
    11281 agagaactca tgcggatagc ggttaatcag gttaggcaat aacccgactt tcagcatcat
    11341 cgccttcacg cgctcgcgaa cttcctggcg tgacattttc ggatgataag tacgcagtgg
    11401 ttcagcgatg atctcgccga tggtcatacg cgggtttagc gatgccaacg gatcctggaa
    11461 aatcatctga atatcactgc gaacggcacg ccattcatcg ggtttcatgc ccagcaactc
    11521 tttacctaac caggcaacat gaccgtcggt cgctttaacc aggccaatga tggcgcgagc
    11581 aaaggtggac ttaccgcatc ccgattcccc taccacacct aatgtttccc cttcatacag
    11641 gcgaagagtt acaccatcta cggctttgag cgttttcggc ggttgccaga accactgttt
    11701 gccatctttg atttcaaagt gcactttcag atcggcgatt tcgaggagga cttttcttcc
    11761 ttcggttaca gcattcataa cagttcctcc accggtttaa agcaagcacg cagacggcca
    11821 ggcgtaaact cttccagcgg cggtgcgcta ctacaaattt ccatcgcatg cggacaacgt
    11881 ggctggaatg ggcaaccttt cggtaatcgc agcaggtttg gcggattacc cggaatagtc
    11941 aacattgttt caccttccgc atcgagacga ggcaccgcgt tgagcaaacc gatagaataa
    12001 ggatgaacgg gttgataaaa gacatcgcgc gcgttgccat attccatcgt gcgcccggcg
    12061 tacattacca gcactttgtc gcagatcccc gccaccacgc caagatcgtg ggtgatcata
    12121 atgatggcgg tattaaattc ccgcttcagt tcattcaata gcgtcatgat ctgcgcctgt
    12181 acggtgacgt ccagcgcagt agtgggttca tccgcaatca gcagcttagg tcgacatagc
    12241 aatgccatcg caatcataac tcgctgacgc atgccgccag aaaattcgtg cgggtacatt
    12301 ttcatgcgtt tacgtgcttc cggcattttt accgcatcga gcatccgcac cgactcttca
    12361 aacgcctcag ctttgctcat gttcttatgc agcatcagca cttccatcaa ctgctcaccg
    12421 acgcgcatat agggattcaa cgaagtcatt gggtcctgaa aaatcattga gatttgttca
    12481 gcgcgcagtt tattgagttc acgctctggc aaattgagga tttcacgccc attgaaggtt
    12541 gccgatccgc caatgcgtcc gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc
    12601 gatttacccg aaccagactc acctacaatg cccagcgttt ctccggcacg tagggaaaaa
    12661 tttaaatcat tgaccgccgt gacgtcgccg tccggggtac taaaggtgac acgcaaatct
    12721 ttcacgttca gcagtgcgtc agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt
    12781 acgctcatgg ctgcactcct taacgatctt tcgggtcgag ggcatcacgc aagccatcgc
    12841 cgataaagtt gaaacaaaac agcgtcacca cgaggaatcc cgctgggaac aacagtaacc
    12901 atggagagac ttccattgag ttcgcgccat cactcagcaa tgccccccag ctgcttaacg
    12961 gctcttgcgt acccaacccc aggaagctaa ggaaagattc aaagaggatc atgctgggca
    13021 ccagtagcga tgcgtagacc accaccacac cgagtacgtt cggcacaatg tggcgaataa
    13081 caatgcccga cgtcgataca ccgccaactt gtgccgcctc aataaactct ttgcgcttca
    13141 gactcagggt ttgcccacgc acaatacgag ccatatccag ccaggaaacc atgcctatcg
    13201 ccacgaaaat cagcaggatg ttttgaccga aaaaggtcac cagcaaaatg acgaagaaca
    13261 tgaatgggaa ggagttgagg atttccagca aacgcatcat taccgaatcc actttaccgc
    13321 ccagataacc ggaaagcgaa ccataaagtg tccccacgac cactgccacc agtgccgcag
    13381 caacaccgac catgagtgag atacgtccgc caatcgcaac gcgcacaagc aggtcgcgac
    13441 cggatgagtc agtaccaaag tagtgaccgg actccatatc cggggcgctg gacatcatcg
    13501 cccagtcagt atcgtcatag gcaaactgcg aaagcatcgg tgccaggatt acaaataacg
    13561 cgatcagcac cagcactatc agacttgcaa ccgccgcacg gttatgcata aaacgtcgac
    13621 gtgcgtcctg ccacaagctg cgcccttcga cctccagctt ttcactgaaa ttttccagcg
    13681 tctcgctgtt tttcttactt aacatcatcg cgagctccag tatcagtaac ggattttcgg
    13741 gtcgataacc gcatatagca catcgacaat ggcattaaac aaaatggtta acgcaccaac
    13801 caggatggtc aggcttaaca ctaaggaata gtcacggttc aatgcgccat taacgaacag
    13861 ttgcccaatc cccggcaaac cataaatggt ttcgataacc atagaaccgg taataatgcc
    13921 gacaaatgcg gggcccatat aggagagcac gggtaacaga gcaggtttta atgcgtggcg
    13981 taaaatgatc cgccgcatag gtaacccttt cgcccgggca gtacgaataa agttggagtg
    14041 taatacttca atcatagagc cacgggtaat acgcgcaata ctggcgatat aggcgagtga
    14101 caacgccacc atcggcaata tcatgaattt aagcgcccca ccattccagc caccgcccgg
    14161 cagccaatgc aaaatgatcg caaatatcat gactaataat ggcgcaacca caaaactggg
    14221 gataacaacc ccggtcattg ccagccccat cacggtatag tcccatttgg tgttttgttt
    14281 taatgcggca ataacgccag cactaacacc cagtattacc gcaaggaaaa atgctgcggc
    14341 tcccagtttg gcagaaacgg gaaaactgga tgcaaccagg tcattgaccg aataatcttt
    14401 atatttaaac gatggaccga aatcgccatg cgccagttgt ttcaggtagc tgaaatactg
    14461 tgtcatgatc ggatcattaa gatgatattt cgcttcgata ttggccatta cttccggcgg
    14521 taaagtacgt tcgccggtaa aagggcttcc cggcgcgagg cgcatcataa agaacgaaat
    14581 agtaataaga ataaatagcg tcggaatcgc ttccagacag cgacgtagaa taaattttaa
    14641 cattgcccta ccttctggcc tgtgccttct gtaatgcgat aaaaatcaga caccgtggag
    14701 caggacactc ctgccccacg tattgccatc agtgcttcac aatgtacata ttccgggtgt
    14761 aggtattatc cagcggatct ttgccggtat aaccaccaac ccacggtttc accagacgcg
    14821 cattcacgta gtaataaaca ggaacaatgg ccgaatcctt atccagctgt tgttctgctt
    14881 tagtgtacag agctgtgcgc tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc
    14941 tgtcaaaggc cgggctctta taatgcgcgg tattcatcga gctgttcgac agcatggtgt
    15001 tcaggaagga agttggttcg ttgtagtcag cacaccagcc tgcacgggcc acatcaaaag
    15061 taccctggtg acgggtgtcg aggaacgttt tccactcctg gttaaccagt ttgacgttta
    15121 caccaatgtt tttcttccac aatgaagagg cagcaatcgc cagcttttta tgcagatcgg
    15181 aggtgttata caacaggttg atggtcaacg gtttgtctgc ggtataaccc gcttcagcca
    15241 gcagtttttt cgcttcttcg ttacgttttt cctggctcca gccaaaccat tccggctgag
    15301 tcaattttgc gccatcagta tacggtggag tgtaaccata ggcgggcatg tcgccctgcg
    15361 ctttcacttt attaacaatg atatcgcggt ccatacccag tttcagtgcg gtacgcacac
    15421 gcacatcgtt gaatggcggt ttctggttgt taatttcgta ataataagtg cacaggtatg
    15481 gatcaacgtg aacttcgtcc gggatctctt ttttcagctt ctggaacaat tcgatcggca
    15541 tgttgttata agtcatgtcg atttcaccac tacggtagcg gttgacatcg gtaacttcag
    15601 aagcaatggg caaataggtt acctggttaa taacggtttt cgcgttgttc cagtaggttg
    15661 ggctgcgttc aagaacgatt cgttcgttta cgacccaatc ttttaaggta taggcaccgt
    15721 tggtgacgat attaccaggc tgggtccatt tttcgccgaa tttctcgata gcggcttttg
    15781 gcaccggtga agttgatggg tgaacaagta atttatagaa gtacggaacg ggttcactta
    15841 aggtgacttc taatgtgtga tcatcaatag ctttcacgcc gagatcggta atcggttttt
    15901 tcccttcaag aatttcatca ataccggcga tatgcccata ttgcagataa ctggcatacg
    15961 gagaagcggt gttcggatca acagaacgtt gccagctata cacaaagtct tgtgctgtga
    16021 ctggcgtgcc atcagaccat ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt
    16081 ctttattatc ccaggattca gcgacgccag gtgccggatg gccgtcaaga tcgctgacca
    16141 gtaagccttc aaacaggtct cggctgatat tagactccgg aacaccttca attttgtgcg
    16201 gatctaatga ctgaacttct gaaccattgt tacgtaccag cgtttgtttt tccgccagtg
    16261 tgacgcctgc gggtacatca gctgccagcg cgacattccc tgccattagc gcagccagaa
    16321 cgccagctgc tactaaactt ctcttggtga tgttggtcat tgttttttgg actccctcat
    16381 tataattact ggtattaacc agcatgtgta atcccggatt gggaccctgc acaggctcgg
    16441 agactttctg ctgtcaggtt actttatcgt tacggattgc tatcaccgac tttatttatt
    16501 ctggtgattc agacgatcac cttatgtcga ttcgtactgt gtctcccctg tcgagacaca
    16561 ttattctttc gcgacgctct ttaa
//
LOCUS       DAZFTN010000100        16550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFTN010000100 DAZFTN010000000
VERSION     DAZFTN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            425..1249
                     /gene="gadX"
                     /locus_tag="ACHKV2_003719"
     CDS             425..1249
                     /gene="gadX"
                     /locus_tag="ACHKV2_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO6941526.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
     gene            1617..2345
                     /gene="gadW"
                     /locus_tag="ACHKV2_003720"
     CDS             1617..2345
                     /gene="gadW"
                     /locus_tag="ACHKV2_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO6941527.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2708..5821)
                     /gene="mdtF"
                     /locus_tag="ACHKV2_003721"
     CDS             complement(2708..5821)
                     /gene="mdtF"
                     /locus_tag="ACHKV2_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO6941528.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5846..6955)
                     /gene="mdtE"
                     /locus_tag="ACHKV2_003722"
     CDS             complement(5846..6955)
                     /gene="mdtE"
                     /locus_tag="ACHKV2_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO6941529.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEVEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7063..7341
                     /locus_tag="ACHKV2_003723"
     CDS             7063..7341
                     /locus_tag="ACHKV2_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941530.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7342..7869)
                     /gene="gadE"
                     /locus_tag="ACHKV2_003724"
     CDS             complement(7342..7869)
                     /gene="gadE"
                     /locus_tag="ACHKV2_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6941531.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8668..9240)
                     /gene="hdeD"
                     /locus_tag="ACHKV2_003725"
     CDS             complement(8668..9240)
                     /gene="hdeD"
                     /locus_tag="ACHKV2_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO6941532.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9495..9827
                     /gene="hdeA"
                     /locus_tag="ACHKV2_003726"
     CDS             9495..9827
                     /gene="hdeA"
                     /locus_tag="ACHKV2_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO6941533.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9943..10269
                     /gene="hdeB"
                     /locus_tag="ACHKV2_003727"
     CDS             9943..10269
                     /gene="hdeB"
                     /locus_tag="ACHKV2_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO6941534.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10333..10980
                     /gene="yhiD"
                     /locus_tag="ACHKV2_003728"
     CDS             10333..10980
                     /gene="yhiD"
                     /locus_tag="ACHKV2_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO6941535.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11032..11802)
                     /locus_tag="ACHKV2_003729"
     CDS             complement(11032..11802)
                     /locus_tag="ACHKV2_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO6941536.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(11799..12791)
                     /gene="shuU"
                     /locus_tag="ACHKV2_003730"
                     /gene_synonym="chuU"
     CDS             complement(11799..12791)
                     /gene="shuU"
                     /locus_tag="ACHKV2_003730"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO6941537.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(12840..13463)
                     /gene="chuY"
                     /locus_tag="ACHKV2_003731"
     CDS             complement(12840..13463)
                     /gene="chuY"
                     /locus_tag="ACHKV2_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO6941538.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13463..13957)
                     /gene="hutX"
                     /locus_tag="ACHKV2_003732"
     CDS             complement(13463..13957)
                     /gene="hutX"
                     /locus_tag="ACHKV2_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO6941539.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(13970..15307)
                     /gene="hutW"
                     /locus_tag="ACHKV2_003733"
     CDS             complement(13970..15307)
                     /gene="hutW"
                     /locus_tag="ACHKV2_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO6941540.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(15327..16241)
                     /gene="shuT"
                     /locus_tag="ACHKV2_003734"
     CDS             complement(15327..16241)
                     /gene="shuT"
                     /locus_tag="ACHKV2_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO6941541.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         4386 a         3880 c         3940 g         4344 t
ORIGIN      
        1 gatcacccga aactgcaggg tattgcccag cagaacagct ttaaacacac ctgataacat
       61 aacgttgtaa aaaccgaatg cccagccttt aaaaaaaaca gctgggcatt cggttgctta
      121 ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata tgttatatga
      181 atgtttatta aatgtagcga ttacctttaa tgtcacgaca agggattatt tgcttactat
      241 taatttccct gcgttcttaa acacgttttt gtattataaa tccagtcatc ctgcccgtag
      301 aacatcacac attatcatcc tgttctcccg cttcgtttaa atttatttat caatcaattt
      361 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
      421 aactatgcaa tcattacatg ggaattgtct aattgcgtac gcaagacata aatatattct
      481 caccatggtt aatggtgaat atcgctattt taatggcggt gacctggttt ttgcggatgc
      541 aagccaaatt cgagtagata agtgtgttga aaattttgta ttagtatcaa gggatacgct
      601 ttcattattt ctcccgatgc tcaaggagga ggcattaaat cttcatgcac ataaaaaagt
      661 ttcttcatta ctcgttcatc actgtagcag agatattcct gtttttcagg aagttgcgca
      721 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaagag cattaatctt
      781 tgcgttgtta tctgtttttc ttgaggatga tcactttata ccgctgcttc tgaacgtttt
      841 acaaccgaac atgcgaacac gagtttgtac ggttatcaat aataatatcg ctcatgagtg
      901 gacactagcc cgaatcgcca gcgagctgtt gatgagtcca agtctgttaa agaaaaaatt
      961 acgcgaagaa gagacatcat attcacagtt gcttactgag tgtagaatgc aacgtgcttt
     1021 gcaacttatt gttatacatg gtttttcaat taagcgagtc gcagtgtcct gtggatatca
     1081 cagcgtgtcg tatttcattt acgtctttcg aaattattat gggatgacgc ccacagagta
     1141 tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg gcggcaagta ttgttgcgca
     1201 agggaatttt tacggcactg actgttctgc ggaaggaata agattataga gttttactca
     1261 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
     1321 ataaaccgtt ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat
     1381 ataactttta ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgc
     1441 gaaaatatat tcgtgttgca tttacttatt atcaattaac tgttatgcaa aactactttg
     1501 tggataaatt ttggtcctac caaatttggc agtttttgcg ctaagaaaca gtctggcatc
     1561 atttcattag tatactgaaa ttgaaataat cgcagtatga aatataaggg ataatcatga
     1621 ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga tatttatcat gaacagcaaa
     1681 aaatatcgct gcataacgag agtatcctgc tgctggagaa aaatttggca gacgattttg
     1741 cgttttgttc accggatacg cgacgactgg atatcgatga gctgacagtt tgccattact
     1801 tacaaaatat tcgtcagcta ccacgcaatt tagggttaca tagcaaagac cgtctgttaa
     1861 ttaaccagtc accccccatg ccgctggtga cggcgatttt tgatagcttc aatgaatccg
     1921 gggtaaattc accgatactg agcaatatgc tctacctttc ctgtttatcg atgttttctc
     1981 ataagaaaga actgatcccc ttacttttca atagcatcag cactgtttca ggaaaagttg
     2041 aacgccttat tagctttgat atcgccaaac gttggtatct gcgcgatatc gcggaaagaa
     2101 tgtataccag cgagagtctc atcaaaaaaa agttgcagga tgaaaacacc tgtttcagta
     2161 aaatattact cgcctccagg atgtcgatgg ccagacgatt actcgagtta cgtcaaattc
     2221 ctctgcatac tattgcggaa aaatgtggct atagcagtac gtcgtacttt ataaacacat
     2281 ttcgacaata ttatggtgta acgccacatc agtttgcgca acattcgcca ggtacctttt
     2341 cctgacatat tttgcatttg aatattggtc aggatctcac acctgcttca tgtgaaactc
     2401 ttccctgatg atttctgccg ggctaccggc tagttctctt tcgcagtttg ccaggcggta
     2461 taggtatttt caggaggaaa agactcggca tgtttgggat tattaagctg acaattcata
     2521 cgattacggg aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat ggctggagtg
     2581 gtttctactt tcagtgctgt gtactaagcc tggtattcct gacggttagc tggttgttga
     2641 gtggtgaatg gttagcagga aagagtaagg ctgaaccttc acgttcaacc ttactctcat
     2701 ttacacgtta cgctttttta aagcgggcaa agagatgttc cactacaacg aaaaagaccg
     2761 gaacaaagta aattgccagc actgttgcgg caaacatccc gcccatcacg ccggtaccta
     2821 ccgcgttttg cgcgccagaa ccggcaccat gactgataac cagcggcagc acgccgagaa
     2881 taaaggccag agaggtcatc aggattgggc gtaaacgcat ccgcgccgct tcgatgattg
     2941 cctctatcgg cgttttccct tctttctgca tcatctcaac ggcaaattcg acaatcagga
     3001 tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag tagacgtcat
     3061 tacttaagcc gcgcagatcg gtggccagta atgcgccaac gacgcctaac ggaacaacca
     3121 acatcaccga gaacggaatt gaccagctct cataaagtgc ggcgagggcg aggaacacca
     3181 cgaccagtga aatcgcatac agcgcaggag cctgatttga ggataacgct tcctgatacg
     3241 acagtccggt ccatgagtag ccgacgcctg ccggaagttt agcgaccagg tctgccataa
     3301 atttcatggc gtcaccggta cttttcccgg ccgccgcttc acctaaaatc tccattgacg
     3361 ggatgccgtt gtagcgttcc agtcgcggtg aaccataggt ccattcggta gacgagtagg
     3421 cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata ccattggttg atgttatccg
     3481 gcaacatacg gaacggcgtg cctgcctgga catacacttt tttcacccgc ccctggttga
     3541 ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat tgtctggttg atatcagaca
     3601 gcgccacacc cattgcctca gctttcgcgg cgttgacgtt cactttgaac atcggcgtat
     3661 cttccaggcc gttcgggcgt accccggtga cctgattcgg tgattgcgct gccagtgata
     3721 acagctcgtt tcgtgcctgg gttagttttt cgtgccccag gttaccgttg tccagcagtt
     3781 ccatatcaaa acctgacgcg gtacccagtt cagccaccgc gggtaagttg aacgggaaga
     3841 cgacggcttt attgatactg cttagcgcaa tcattgcccg ctgaatgatc gcggtaaccg
     3901 agttttcctc accgacacgt tcagaccacg gcttgagact gataaacgcc agaccgttgt
     3961 tttgcccctg gccgctgaag ccaaagccgc caacggtaaa caccgactga acattatctt
     4021 tctcttttgt aagataataa tccgtcacct gttgcagcac tttcgtggtg ttaaccatgg
     4081 tggcaccgga aggtaactgc gcggtggtca taaatacccc ctgatcctct tctggtaaga
     4141 aagaggtcgg cgtgcgcagg aacagcaccg ccatcccggc gcaaatcagc aggtagatca
     4201 ccatgtagcg accggtacaa cgcaacagtg agcgggtgct atcggtatag tgctgagttg
     4261 atttttcaaa cagcgtgttg aagcgtgcga acagggcatt aggtttgtga ccgccttccg
     4321 gcgcggcttt cagaatggtg gcgcacaggg caggggtcag gctcattgcc acaaatactg
     4381 aaagcagcat ggaggagatc agcgtgatgg agaactggcg gtagatctcc ccggttgcac
     4441 cgctcataaa ggccatcggc ataaacactg cggaaagaac aacggcaata ccgaccagcg
     4501 cacgttggat ctgccccatc gatttatgcg tcgcttcctt cggtggtagc ttatcttccg
     4561 caatgacacg ctcgacgttc tccaccacca cgatggcgtc atccaccagt aaccctatcg
     4621 ccagcaccat cccgaacata gtcaacgtgt tgatggtgaa accgaccgcc gacaagatcg
     4681 caaacgtccc gagaataacc accggtacgg caatcgtcgg gatgattgtg gcacggaaat
     4741 tctgcaaaaa cagatacatg accaggaaga cgaggatgat agcctcaacc agtgttttga
     4801 aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa accgtcttca
     4861 gacttgccgg gaaataggct gataagcggt tcagttcctc tttgactgcc cgcgaggtat
     4921 ccagggcgtt tgctccggta gccagtttga tggcgatccc ggcagcaggt ttgccgttat
     4981 agcgcgccac ggtggaataa tcttccgccc caagttcgac gcgggcgaca tcacgcagca
     5041 gcacttgcga accatcttgc tgaactttca acaggatttt gccaaattct tccggcgttt
     5101 gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg gtctgccgcc tgtggcatgc
     5161 cacccagttg accaccagaa atctggttgt tttgcacctt aatctgggaa ataacatcgg
     5221 aaggtaccag attgtattta ttgagttttt gcgggtccag ccagatacgc atggcgtact
     5281 cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg tctttgatat
     5341 tagacgctac atagtccgcg atatcgtact ggttgaggct gccgttatca gaaataaacg
     5401 ccgctaccat caggatatta ctgctcgatt tatcgacgct aatcccctgc tgctgcactg
     5461 cttcaggtaa tgaaggcata gcgagttgca gtttattttg cacctgaacc tgtgcgatat
     5521 caggagatgt cccagtctcg aaggtcagag tgatagaggc attacccgcc gcatcactgg
     5581 ttgaagacat gtacatcagg ccatcaagcc cattcatatt ttgctcaatc acctgagtga
     5641 ccgagtcttc taccgtttgc gcatcggcac ccggataggt agcgctgacg gtaatggtcg
     5701 gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga ccacctgcaa
     5761 gcatcataat aatggcaagt acccaggcaa aaaccgggcg atcaataaaa tagttagcca
     5821 tgaaagtccc cttaagcctg caacgttatt gtttcgattc ggtgctggcg ttttcctggc
     5881 tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg gaaacgatca
     5941 cccgatcgcc agcctgcaag ccagaggtga caacccactg gtcgccgatg gctttgctgg
     6001 cttcaatttc gcgtagcttc acgacatcgt ctttatccag aatgagcgcc gttgctttac
     6061 cttgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg cggctacctt
     6121 catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg ttggggaaaa
     6181 tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag aatttcagcg
     6241 tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc gtactgccct
     6301 gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa tcttgcaccg
     6361 actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct
     6421 gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg
     6481 gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga
     6541 cggtgacatt ggcttctact tcattcaact gggtgcgcgc ggtgtcgtaa tcctgacggc
     6601 taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg
     6661 cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc tgtaaaggtg
     6721 caggatcaat ctgatacagc gaatcgccct ggttcacttt atcgccttcg ataaagttgc
     6781 gtttaatgat aataccgccc acctggggac gtatctcggc aacttcataa ggaacggttc
     6841 taccgggcaa ttcgctcaac acattgaccg acccagggga gagtgtgacg acaccgacct
     6901 caggcgtcat ggcggcggtg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc
     6961 cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt
     7021 cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc
     7081 agcggcatag tggggttgtc agccagtaag gtgggataca ggcatagtga tcgacatggt
     7141 gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca
     7201 acgaagggtt ctactggtgg atacacatac caggggaata aagtcgtttt agcctctcct
     7261 ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa
     7321 tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct
     7381 catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg
     7441 cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat
     7501 aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca agtgtttacc
     7561 ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat aaataggcgc
     7621 tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa
     7681 tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat
     7741 aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat
     7801 ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag
     7861 aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct tttcattatt
     7921 tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg
     7981 tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat
     8041 atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa acaaaaagtt
     8101 aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag
     8161 aatattacta aagttaaaaa gtaatccgat ttaaatatcg agtctccttg tttcgactta
     8221 agctggcaat tggattgcca gctttctttt aacgcattga aattacctga gtaaataacg
     8281 cattcatgtc ttcaggtttt tatatagcgt ctggagacat ggatatttat gcaatgtaat
     8341 caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat agcatcaacg
     8401 cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac ctgactacgg
     8461 aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga aaaaaattag
     8521 atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga tagtgcgcaa
     8581 ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca catcaagtga
     8641 ctatgatccg ggtgacaacc gggataatta ttgctgctta acgaacaaac tggcgaagct
     8701 gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa ccagcgttac
     8761 agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca gcacaccaat
     8821 aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca tcaggcgaat
     8881 aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa tgcccagctc
     8941 cggtgcgcgg atgaagaaat agccgatcaa taaataggcg actgcgacga ggaaaccgga
     9001 taataccggc cagaaattat gactgcggtt gctgaataat ccaacaataa gcgcaatgcc
     9061 cgaacagatt aataatgcac ctactactgt gcttaaaata tcgccagaaa cgaacgggaa
     9121 actgatacac agcaacccga cgataaacag cagcacggca ataaactgga ttgctctgcg
     9181 atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta tatataacat
     9241 agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc caaaaacgcg
     9301 tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat gcatctgtaa
     9361 ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca
     9421 gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt cactttatag
     9481 ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg cttcttctgc
     9541 cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa ccggtcaact
     9601 cctggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact gcagttggtt
     9661 ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat gttcagggta
     9721 ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa gccaacttta
     9781 aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg
     9841 ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa agcagctatt
     9901 cctcctgttc atatataatc tctatattga atgggttaca aaataaatat ttcatctctc
     9961 cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa cgcacaatct
    10021 gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat tgatctgaat
    10081 ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt atataaaggt
    10141 ggcgatacag ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac
    10201 tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg
    10261 ccgaattaat gaggtacaag taaaaaggag tggcaagttg agccatcttg ctgctccttt
    10321 ttgcattttt atatgacagc agaatttatt atacgtctta tactcgcggc aattgcctgt
    10381 ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cactcatgta
    10441 ttaattggca tgggaagcgc actgtttatg attgtttcga aatatggttt tgctgacgtg
    10501 ctgtctttag atcacgtcgg actggacccc agccgtattg ctgctcaggt ggtgacgggc
    10561 gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtttgacg
    10621 acggcagcgg atatctgggt gaccgccgcc ataggtatgg ttattggcag tggtatgtac
    10681 gaactgggta tttatggttc agtgatgacc ttgctggtgc tggaagtctt ccatcaatta
    10741 accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa cggtaatacg
    10801 gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt ttcgttgcag
    10861 gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac aacgtcgata
    10921 gaagatctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc aattagctaa
    10981 tctgtatagt tatttattcg caggagtacc agtgcggtac tcctgttgct tttaatgatc
    11041 gaggacgatc agtggtgaat ggttggcagg atctttaagt acggtaatat ccgcgccgta
    11101 gagcattgtc agcgcctgct gtgtcaatac gtcctgtggt ttaccatttg cgataacctt
    11161 tcctttttgc attaatacaa cacggtctgc ataatgcgcg gcaagattta gatcgtgcaa
    11221 tatgcagcag acattgaact gccgctcgtg aactaactgg cgtaataaac gaaacaagtg
    11281 ctgctgatgg tgaatatcca gcgccgaagt tggttcatca agaaataacc atttcggtga
    11341 gggagttggt tcccagagtt gcaccagcag gcgcgctaat tgcacgcgct gttgttcacc
    11401 accggataat tgccgatagt cgcggtttgc taacgcctga cagtcacata acgccattat
    11461 ttgtgctgtt tcattatcct gattaccagt acgatgcgga tgccgaccca tctggataac
    11521 ttcttgtacg ctgaatggaa acgccatgtg gctattttga cgcatcacgg cccggtgttt
    11581 cgccagttca gtaatcgacc attcatttaa tggcttatta aacaaccgac attcgccgct
    11641 atcaggttga agatagccgg ttaactggcg aagcagcgta gattttcccg caccattagg
    11701 gccgagaatg gcaacaattt ccccaccggg aaaagtcagc gagacattat cagtcagacg
    11761 ccgcccctga aggctataaa ccagattctg ggcgctgatc atgtgatcct ccgatttcgc
    11821 agaatcaacc acataaaata agggccacca agcaggctgg ttaataatcc cacgggcatt
    11881 tctgctggct gtaccagcgt gcgggcaagc gtgtctgcca tcagcaataa acaggctccg
    11941 gcgagggcgg aacaagggat gagccagcgg tgatttgctc cggtagtcat gcgaatcaga
    12001 tgcgggatca ccaggccaat aaagccgata atgccgctta ccgatacggc agcaccaacg
    12061 agcagtgagc tcactaatag taattgctgg cgtttacgct taacgttcac gccgaggtaa
    12121 tgggcttctt catcaccgag ctgcagtaaa ttcagcgttc cggcgagaca agttgttgcg
    12181 ataatggccg gaaggatgaa tgaactggca accaataacg tcggccattg cgcctgtcca
    12241 agattgccca tcatccacaa tgtgagttgt cgtagctgct gctcatcgcc aatatagctc
    12301 aggatgccga tcgctgcgcc gcacagggca ttgatggcga taccggcgag taataattgc
    12361 atcatgctgc catcgcgatg gcgctgcgtg atgagaaaaa tgatggcgca gactactaaa
    12421 cttccggcaa tggcaaacac catttgctca tagagcacca gcaccactgg gaaggagaag
    12481 ggcagcacaa ttgcaacgcc aaccattaat gcagaaccgc tactgacgcc gagtaatcct
    12541 gggtcagcca ttgggttgcg aaaaagcccc tgcataatta cccctgcggt tgccaacgct
    12601 gcacctacca gcaccgccag caaaacccgt ggtagacgaa tattgagcca gatatcgcgg
    12661 tattcctcat caaaaagggc atcaaaactg atctttaacg ctccctgact agcaccgaac
    12721 agcaccaggg caagcaataa tagtgatagt cccataaaga ccctggcgga tgaaaatgaa
    12781 tccttgagca cgcaaaactc cgttacgaca ttacggcgcg gacatgccgc gccggggtat
    12841 cacgccggtt tcaggtccgg ttcaatcaga ctgtagacct gctggttaag tgctggcgca
    12901 tttgccagtt catggatatg tgcggcgaca tcggcacggc ggacaaaacc atgacactcc
    12961 tgattttgta tccgttgtgc tttgcctgtt gctgcgccgt caagcagacc accagggcgg
    13021 agaatggcgt agtctagctg gctggtttgc agccagcttt ccgccagcgt tttttcccgt
    13081 accgcctgac caaacgcagc ctttgcccgc tccgataaaa acggccagct atcgccgcat
    13141 cccaaagagg tcaccagaat catgcgactg atgccggcct tttcggcctc atcaatcact
    13201 gttctgtgcg ccagataatc ctgagctcct cccattgttg agatgataag cgcatccggg
    13261 ccagctgcgc ggcaagcagc cgcgactacg ctggcatcac aggcgtcgcc tgtaaaaacc
    13321 tgtacgcctt gttgcgccag tttcgtggcg gcatcggcat gacgaatgac agcaacgacc
    13381 ggacgttgtt ccgccagcgc cagttcaagc gttctggcac ccacaccttt tccgcctgcg
    13441 ccaaagagta gccacggcgt catcaggcgt gttcctttaa cgatgccgcc agagtatgga
    13501 aggcgctgac ttgttcgctc aggagctgac gatggtcatc cctgccgagg aagattttca
    13561 gcatggcgct gccttctttg ttgaaaaaga gaatggacgc ggtgtccatg cccataaatt
    13621 tacgttcgat taaggcaata tgcgtgcagt tttctgcttt gatatgcccg gacatgccgt
    13681 gctttccgcg taggttgaaa tagccgtggc gatggaaacc cgaaggcagt tcgccgctaa
    13741 attcgaggat tacatcggca gtatgtacta acgtggtgac gttgccccat tcgcagacgg
    13801 tatcccagac agtatcgaat ttatcgcctg gcactacggt agaggagggt agatttctca
    13861 ctacctccag cagtgtggtg ttgtattgtt cggctaccac ctccagcgta ccgtccggct
    13921 ccgttttcaa aaattcttgt aacgagacat ggctcataaa tcactcctgt tatggttttt
    13981 cacgcataat cgctggcgca ttaagtacct gaattagttc attaagagac tgcaaaatat
    14041 tgcttgccca gaaacgacct tcattagtca gccgcaggca agttgaagca tcgcgactta
    14101 agcctttttg gtgccattga gccagtaacg gcgcgagttt ttcggcatgt ggcgtaagtt
    14161 catccagcgg tacgcgcgct gtttcaacac ctgactgcaa ggtatgacgc cattgcgcat
    14221 tacgttcggc gttacgcatg atcaacatca gcggtttttt gcctgccgcg acggattcat
    14281 gccaggtctg aagattgcgt tcgttcatcc aggagtaacc attaatcgaa ccgccggctc
    14341 cggaaccaaa ggccagacaa tcggcacctt gtttaatcag caggttatag agattgcgtt
    14401 cgcgtgtggt acggccccag tggctgttac tgatgcagcg ccaaccggca tcgtccataa
    14461 aatcacatcc ttgcagataa agatcgcgac gttctgccgg tgagggtacg gtagtacgcc
    14521 cattttccac ggccttgccc agcggtgtat tggacaggac attgagcgca tagagatcga
    14581 cgccgtcgag accgatatcg cgggcaatag ccagatcttc tccccaggtt tgcgcatcct
    14641 gacccggcag gccaaacagc aggtcacaga ccaccgcagc acggtcacgt ttgaccaggc
    14701 tttccataaa cgcaatagcg gttgggccat ccgaggtgcg ggccattttc ttgcggattt
    14761 tgctgttaaa cgactggata ccaattgaga agcggtttgc tcctgcatca aggcaagcat
    14821 cgattcgctc agcgtcaaaa ttcaataccc gtccttcgat agtgatttca caatcagggg
    14881 ccagtggcag cttttctcgt aatgtggtga tgattctggc caaatcgtgt gccgaaagtg
    14941 cggaaggcgt accgccaccg aaatagaccg cgtggatggg ggcagactga tgcaatacgc
    15001 tgtccgcttc catttctatt tcacgaatca gggcgtcggt ataatgagca cacgcatctt
    15061 cattaaaacg gttctgataa aaaccacaaa acgtgcagtg tgtcgcacaa aaaggaatgt
    15121 gtaggtaaac cagtcgctta cgaggtgatg ccgtttgatt gatcatctct tgccaggtct
    15181 gtgccagttg ctctttggca accggaatgg ctccacgaaa cggcatcatg gcgcgcctgt
    15241 ctttaaatgg ctgatcaccg tctaaagcaa aatgtggcgt gagatccagg gtgttatttg
    15301 cgttcattcg tctgatttaa atccatttat tgcggccaaa gctgctggtg taaagactca
    15361 acgacatcag caatacgcgg acccattccc agaatcaggt tttgatcaac ggtgataatg
    15421 cgctggtttt tccatgcggc agtgtgggta attccagcaa tagaacgtag ccgattaata
    15481 tcgccatcga ccatttgtga ggttacgaca atcacttctg gattagccgc aatcagtgat
    15541 tctgcactgt agcttttgta ctgctggtgg gtagcgacgt tttctgctcc ggcaagcgaa
    15601 agaatggcgt ccgcgacgct gcctttaccg gcaacttgtg gcgcacttcc gcctgcggag
    15661 agaataaaca tggctttaac cggggctttt ttggtcgcca cgttttgctg tactcgctcc
    15721 aggcgttggc tgatttgtgt caccagcgcc tcgccttgtt caggaacctg tagcgttttt
    15781 gccagttggc gaatgttggc gtacatctgc tgaagggtgg caggcacacg cggcagggtg
    15841 acgacgttga ctttctgcgc tcgtagttgg tcgagcacaa tttgtggccc ggcatcctgc
    15901 caggtaatta cgctatccgg gcgaagtgac aaaatgcctt cgctgctgag ttgtttccag
    15961 tagccaatat gcggcagttt ggcggtttct ggtggataag atgtcgtttc atcgacacca
    16021 accacgcgtt cgccagcgcc catcgcgtag attagctccg tcagcgatcc tcctgcgacc
    16081 acgatacgtt cggcagccgt tacgctaaaa gcccagctaa gagccagtac tgccgtaaac
    16141 aattttttac gttttactgc ggtcttcgcg gcagaggcca cggctgaaat acataacgtc
    16201 agtggggaaa aaagaaacgg cctggtgatg atccttggca taatatgaga actcttatca
    16261 aattgagaat gatcatgatt atcatttgag ttatatatga aatacaaccg tcgattcaat
    16321 acgcaaggcg ttacaagcgt atttattttt gatttttatc aagatgcttc gtcagctaac
    16381 gttcttgttt ccgactaaat ttcatcgcgg gaaaaagctg agaacccaga gatatcgagg
    16441 cttgcagagc aaagagaaga aaaaattatc actgttaacg acaaaattta gtgatgaggc
    16501 atggggggat aaccataaaa acaaggtggt ttacagggcg caggataggt
//
LOCUS       DAZFTN010000101        16516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFTN010000101 DAZFTN010000000
VERSION     DAZFTN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(168..1451)
                     /gene="yeaH"
                     /locus_tag="ACHKV2_003735"
     CDS             complement(168..1451)
                     /gene="yeaH"
                     /locus_tag="ACHKV2_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO6941542.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1564..3498)
                     /gene="yeaG"
                     /locus_tag="ACHKV2_003736"
     CDS             complement(1564..3498)
                     /gene="yeaG"
                     /locus_tag="ACHKV2_003736"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO6941543.1"
                     /translation="MNIFDHYRQRYDAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3934..4680
                     /gene="mipA"
                     /locus_tag="ACHKV2_003737"
     CDS             3934..4680
                     /gene="mipA"
                     /locus_tag="ACHKV2_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO6941544.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4770..5624
                     /gene="yeaE"
                     /locus_tag="ACHKV2_003738"
     CDS             4770..5624
                     /gene="yeaE"
                     /locus_tag="ACHKV2_003738"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO6941545.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5675..6559)
                     /gene="yeaD"
                     /locus_tag="ACHKV2_003739"
     CDS             complement(5675..6559)
                     /gene="yeaD"
                     /locus_tag="ACHKV2_003739"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO6941546.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6643..7638)
                     /gene="gapA"
                     /locus_tag="ACHKV2_003740"
     CDS             complement(6643..7638)
                     /gene="gapA"
                     /locus_tag="ACHKV2_003740"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6941547.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7980..8393
                     /gene="msrB"
                     /locus_tag="ACHKV2_003741"
     CDS             7980..8393
                     /gene="msrB"
                     /locus_tag="ACHKV2_003741"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO6941548.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8435..8707
                     /gene="yeaC"
                     /locus_tag="ACHKV2_003742"
     CDS             8435..8707
                     /gene="yeaC"
                     /locus_tag="ACHKV2_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO6941549.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9077..10153
                     /gene="ydjL"
                     /locus_tag="ACHKV2_003743"
     CDS             9077..10153
                     /gene="ydjL"
                     /locus_tag="ACHKV2_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6941550.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10180..11559
                     /gene="ydjK"
                     /locus_tag="ACHKV2_003744"
     CDS             10180..11559
                     /gene="ydjK"
                     /locus_tag="ACHKV2_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6941551.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11576..12619
                     /gene="ydjJ"
                     /locus_tag="ACHKV2_003745"
     CDS             11576..12619
                     /gene="ydjJ"
                     /locus_tag="ACHKV2_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO6941552.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12640..13476
                     /gene="ydjI"
                     /locus_tag="ACHKV2_003746"
     CDS             12640..13476
                     /gene="ydjI"
                     /locus_tag="ACHKV2_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO6941553.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13481..14413
                     /gene="ydjH"
                     /locus_tag="ACHKV2_003747"
     CDS             13481..14413
                     /gene="ydjH"
                     /locus_tag="ACHKV2_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6941554.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHCTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14423..15403
                     /gene="ydjG"
                     /locus_tag="ACHKV2_003748"
     CDS             14423..15403
                     /gene="ydjG"
                     /locus_tag="ACHKV2_003748"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO6941555.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15540..16298
                     /gene="ydjF"
                     /locus_tag="ACHKV2_003749"
     CDS             15540..16298
                     /gene="ydjF"
                     /locus_tag="ACHKV2_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6941556.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16416..>16516
                     /locus_tag="ACHKV2_003750"
     CDS             16416..>16516
                     /locus_tag="ACHKV2_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941557.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
BASE COUNT         4289 a         3713 c         4168 g         4346 t
ORIGIN      
        1 aatacgtgtt gacagaataa tttttccgaa tgattgtttt ctggaactaa attgtgtaaa
       61 ggttatgctg tcatatttaa agcgattgta agctaatgta tgtaataaat gagataactt
      121 acagtgagct aaaaatcagc caggcgataa tgacccggct gatagtttta gtctttagct
      181 gttgcatttt gcttatgaaa cagttcacgg aacaccggat aaatatcatc ctggtcgcgg
      241 atatgctgca tcgcaaagtt gtcgaaagta gattgcagat gctcatattc tcgccacagt
      301 gtctgatgcg cgcgacgggt aatttcgata tagctgtaat aacgaacaac gggtaataat
      361 tttttcgcca gaatttcatg gcaaagcgga gagtcatcgg cccagttatc gccgtccgat
      421 gcttgtgcag cgtaaatatt ccactgtgcc gggttataac gctctttcac tacctcatcc
      481 atcagtttca gggcgctgga aacaatggtg ccgccagttt cctgcgagta gaaaaactca
      541 tgttcatcga cttctttcgc ctgggtatga tggcggatgt atacgacttc cacgttctta
      601 tacgttctgc tgaggaacag atacagcaga atataaaaac gcttagccat atctttagtg
      661 gattgatcca ttgaaccgga aacgtccatc aggcaaaaca tcactgcctg gctggaggga
      721 tcgggccgct tctcgtagtt cttgtaacgt aaatcgaagg tgtcaataaa agggacgcgt
      781 tcaattttgg cacgtaattc tgcaatttct ttgcgcagac gttcctcttc cagcagctgc
      841 gcaggttcac tgttgctgat gatggccaaa ttctcttcca gtgcatgaag ttcccgccgc
      901 ttgcctgccg tcatggctgt gcgtcgcgcc agtgagttct gcaaggaacg cacaacgctg
      961 atattggccg gaacgccgtt agcggtataa cccgcccgat gcgttttata ttcggtcagc
     1021 tggcgttgtt ggttttgttt cagattcggt aaggccaaat cttcaaagag cagatcaaga
     1081 tactcatctt tcgaaatctg aaagacaaat tcatcctgac cttcaccatc ctggctggcc
     1141 tggccctgac cactgccgga acctccgccg ccaccctggg gacgttcaat tcggtcgttc
     1201 tgaacgaaat ggtcattgcc cggatgcacg cggtggcgca gaccgccacg cccctgatga
     1261 aacattggtt cgctaatatc ttccgtggga atggatacgg actcgccgct gtcgacgtca
     1321 gtcaccgaac gcttattaat ggcctcggag atcgactgtt taatttgcgc tttataacgg
     1381 cgtaaaaaac gctggcgatt caccatgctt ttgtttttgc cgttcagacg ccggtcaata
     1441 aaccaggtca tatgcccccc gtactacatt tgccaactgt atgctacgcc tgtgttgcca
     1501 tttctctggc ccacggttcg ctgtcgttgt aggcccggta gcatgtgtac cgggccaggg
     1561 ttgttaagac gatttacgta cgcgcaaata ccattcgcac agtaaacgca cctgtttacg
     1621 ggtgtagcct ttttccatca tacggtcgac aaagtcgtcg tgtttcttct gctcgtcggt
     1681 tgacgttttg gcgttaaacg agataaccgg caacagctcc tcggtattgg agaacatttt
     1741 cttctcgata accgtgcgca gtttttcata gctggtccag ttcggattgc gtccgctgtt
     1801 attggctctg gcgcgcagta cgaagttaac aatctcgttg cggaaatctt ttggattact
     1861 gatccccgcc ggtttctcga ttttctccag ctcggcgtta agagactcgc ggtcaaacag
     1921 ctgcccggta tccggatcgc gatactcctg atcctgaatc cagaaatccg cgtaggtaac
     1981 ataacggtcg aaaatgtttt gcccatattc ggaataggat tcaaggtagg ccgtctggat
     2041 ctctttgcca ataaactcgg catatttcgg gatcagataa cctttcagga actccagata
     2101 gcgttctgcc tgctcttgtg ggaactgctc gcgctcgatc tgctgttcca ggacgtagaa
     2161 cagatggacc gggtttgccg ccacttctac atgatcgaag ttgaacacgc gggagaggat
     2221 cttaaacgca aaacgcgtcg acagaccgtt cataccttca tcgacaccgg cgtagtcacg
     2281 atattcctga tacgacttgg ctttgggatc ggtgtctttc agactttcgc catcataaac
     2341 ccgcatcttt gaataaatgc tggagttttc tggctctttc aggcgcgaaa gaatggaaaa
     2401 acgtgacagt gtttcgagag tgccaggggc gcatggggcg tgagtcaatt cactgtgatt
     2461 aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc aagcaatacg gcaccttcac
     2521 gatgtaaaca cgatcgagga aggcttcgtt gtttttgtta ttacggaaag tgacccattc
     2581 ggactcgttc gagtgggcaa gaataatccc gttgaacggc agggcggaga taccttccgt
     2641 cccgttgtag ttaccttcct gagtggcggt taacaaggga tgcagcactt ttatcggtgc
     2701 tttaaacatc tcgacaaatt ccatgatccc ttggttggcg cggcacagcg caccggaata
     2761 gccgtaggcg tccgggtcat tctgcgcgta atgttcgagt ttacgaatat cgactttccc
     2821 aaccagcgcg gagatgtcct ggttgttctc atcaccgggt tccgttttgg cgatagcaat
     2881 ttgttgcaga attgacggcc agaccttcac tacccggaac ttagtgatat cgccaccaaa
     2941 ttcatgcagg cgttttgccg cccacggcga catgatggtg ccgagataac ggcgaggaat
     3001 gccatactct ttttccagaa tctgcgcatc ttcctgcgga ttgaaaagac agaacggatg
     3061 atcgttgacc gggctacgct caccgttggc gctcaataca taaatcggta cgagctgcat
     3121 taatgatttc agtcgctcag caagcgatga tttaccccca cccacaggcc ccagcagata
     3181 caggatttgt ttcttctctt ccagcccctg agccgcgtgt ttcagataag agacaatctg
     3241 ttcaatcgcg tcttccatgc cgtaaaactc ttcaaacgcc ggataacgtg caatgacccg
     3301 gttagaaaag agtcgagaaa gtctgggttc ctgggctgta tcgaccatga caggctcacc
     3361 gatagccatc aatagccgct cagccgcgtt ggcataagca ctgcgatctt gccgacaagt
     3421 ggtaagaaac tcctgcagtg tgaactcttc gtccttggca gcgtcatatc gctggcgata
     3481 gtgatcgaat atattcatgg tatgccgtcc tttcgttttt tagcacaggt taagagccgt
     3541 tcatatgaat agtagaggct cccggaagag gtaaactgaa cgacgtctct tgctaaaaca
     3601 cagcaactct catgccattt tatcgacgat gagaacaacg tgcccggtga tacaccttca
     3661 aaattaagcg tagatggcaa atggaaaact tgcctacacg gctttcttaa tttcaatggc
     3721 atatcaacaa ctcatccata aattttgcat aattaatgta aagaccaggc tcgccagtaa
     3781 cgctaaattc atttggctgt aagcgcggtg tcatccgcgt caggaaaatt aaacagttac
     3841 tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg
     3901 cccgctaaac atttgtttaa ggaatgatta attgtgacca aactcaaact tctggcactt
     3961 ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc
     4021 gcaggcgtag gtgtcgttga acacccatat aaagattacg ataccgatgt ttacccagta
     4081 ccggtaatca actatgaagg cgataacttc tggttccgtg gcttaggtgg tggttactac
     4141 ctgtggaatg acgcaacgga taaactttca attaccgctt actggtcgcc gctttacttc
     4201 aaagctaaag acagtggcga tcaccaaatg cgtcacctgg atgaccgtaa gagcaccatg
     4261 atggctggtc tgtcttatgc tcactttacc cagtacggtt acctgcgtac caccctggct
     4321 ggcgataccc tggataacag caacggcatc gtctgggata tggcctggtt gtatcgttac
     4381 accaacggtg gcctgaccgt gactccgggt attggtgtgc agtggaacag cgaaaaccag
     4441 aacgaatact attatggcgt atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat
     4501 aactcgaacg acagctggag cccttacctg gagctgagcg ccagctacaa cttcctcggc
     4561 gactggagtg tttacggtac cgcgcgctac acccgtctgt ctgatgaagt tactgacagc
     4621 ccgattgtgg ataaatcctg gactggcctg atttctaccg ggatcaccta caaattctga
     4681 taatgcatct ttccagggca ttttgtgcat aaaaacaggg cgtcacgccc tgttttgcat
     4741 tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg
     4801 atgtctcact gccagccata gggcagggaa catggtatat gggcgaagat gccagtcagc
     4861 gcaaaacaga agttgctgca ctacgcgcgg gcattgaact cggtttaacc ctcattgata
     4921 ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt tggggaagca ttaaccggtc
     4981 tgcgagagaa ggtctttctc gtctctaaag tctatccgtg gaatgctggc gggcaaaaag
     5041 cgataaatgc atgcgaagcc agtttacgcc gtctcaatac tgattatctc gatctctact
     5101 tattacactg gtctggcagt ttcgcttttg aagagactgt tgcagtgatg gaaaaattga
     5161 tcgcccaggg aaaaatccgc cgctggggcg tttcgaacct tgattatgct gatatgcagg
     5221 aactctggca gctgccgggg ggaaatcagt gtgccactaa tcaggtgctt taccatctcg
     5281 gttcacgagg gattgagtac gatctactcc cctggtgcca gcaacagcag atgccggtga
     5341 tggcttacag tccgttagcc caggccgggc ggttgcgcaa tggactgtta aaaaacgcgg
     5401 tagtcaacga aattgcacat gctcacaata tcagcgcggc gcaagtattg ttggcgtggg
     5461 taatcagcca tcagggtgtg atggcgattc caaaagcggc cacgattgcc catgtccaac
     5521 agaatgcggc tgtgcttgag gtcgaacttt cgtcagcgga attagccatg ctggacaaag
     5581 cgtatccggc accaaaagga aaaactgcgc tggatatggt gtgatgttga tacgtgccgg
     5641 atgcatcgca cccggcacac aacattgacg caaattaacg tttggcaacg cgaatggatt
     5701 gcgccagatg tgcaggtttt tctttggtca ctttttgcat ttctgaggcg taagctgttt
     5761 ctacacaaac aaaggtcttg tagccatcgt ccggcatgtc gcccatgcta actgaaagcg
     5821 ccggtcccgg gttccagcct acaacgttca gatgatgctg gtggcctacg gcgataatac
     5881 gattcagcgc ttcatcatta atcacgctgc aatcttgcgg attcagatac acgcgatcgg
     5941 tacggtcagg gaaggtctga ataccgtcgg tcagtacatc ttctttcgcg tcatttactt
     6001 tatcaatgaa gcgatcgccc agcccactga cgcttacctt agcgatatca cccacgttaa
     6061 agtaggtatg cagggcagag gtggtttcaa attcgccgtg tgcttcaaga tcgatttcac
     6121 agtttttatc cacgcggaaa tgcgctaaca gagtaaagtc gtgcagccag aattttttcg
     6181 tctcttcgct ttgtgtcaat tcaaaagtca gcgctacgcc attagcatct tcacgatgtg
     6241 atttcagcgt ccacggcagg ttgcgggcaa aaccgtgcgc aggcaggcct tgttgtgccg
     6301 ccggaccaaa ccacggccag caaaccggta cgccaccgcg aatagcaacg ccatttttga
     6361 acggtgtgtt gttgctcaac cacagaactt cttcttcacc tgcaggtttc cacgagagaa
     6421 ggtgtgcgcc ctgtaatgca aaagaggctt ttacctgggg atgatcgact acaatgaggt
     6481 ccagttcatc cagtttacga cgggagagga caggggagat ttgttcgatg accggaaggg
     6541 caaaaatttt cttaatcatg acgcagtcct ttaacttcat tttatcaggt aaaaaaaaga
     6601 gcgaccgaag tcgctctttt tagatcacag tgtcatctca acttatttag agatgtgagc
     6661 gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg ataccagttt
     6721 cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag tgcaaacttc
     6781 gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc ctttcatttc
     6841 gccttcagca gcagctttaa cggcagcttt gatctgctcg taagttgcag ctttttccag
     6901 acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg ccataccagt
     6961 cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac cggtagagga
     7021 cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag acgggccatc
     7081 aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt cgatgatgcc
     7141 gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc aggaagcgtt
     7201 ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga acatcggagt
     7261 gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga tgtgtttacg
     7321 agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa caccaacttc
     7381 gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt taccgttaac
     7441 gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag tggagtcata
     7501 tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa cgatctcgat
     7561 gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa aaccgttgat
     7621 acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa ggttgcctgt
     7681 aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg cgacaaatca
     7741 atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg cttgagacca
     7801 catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga ttttcatcac
     7861 gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc cagttggtgt
     7921 taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga tgtgagcaaa
     7981 tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg cagttttacg
     8041 tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat aacaagcgtg
     8101 acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa accaagtatg
     8161 attccggctg tggctggccc agtttctacg aaccggtgag tgaagaatcc attcgttata
     8221 tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac tgtgatgccc
     8281 atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat tgtgttaact
     8341 ctgcttcttt acgcttcact gatggtgaaa acggcgaaga aatcaacggt tgaaaaaacg
     8401 attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca ttatcaacag
     8461 catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga aatggcctga
     8521 tggcgttgcg ttgaccgagg aacaaaaaga gaactgcctg caactggtga tgctgtggca
     8581 agcccgcaat aataccgaag cacagcatat gacaattgac acaaatggtc agatggtcat
     8641 gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta ttgccatgtt
     8701 taagtaagtg gttcatcccc tttttgccgc ggctatgaag gggatagcca acaatgaccc
     8761 cacgactcat ctcaattatc tcgccatgac ctggctaagt cccagtccga tctcttgccg
     8821 tcgcgttcac gcctgaatga cttctcctga acttaagtct cagacctatt tggccggtaa
     8881 tccctctcaa atttgctcct tttgcgttcc gccttacatc aacctgtaac agaaccagcc
     8941 attttctgcg taattgtgat ctgagttgta aaacgcagat tattatctgt gttttacatg
     9001 tttaatgttg acctcaaaaa tcatgtgggt aagtatgatg accatctaag caaaacaaaa
     9061 cacagaggat gacgcaatga aagcactggc tcggtttggc aaagcctttg gcggctacaa
     9121 gatgatcgat gtgccacaac ccatttgtgg cccggaagat gtagtgattg aaattaaagc
     9181 cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc gatagcggtt ctgatgagtt
     9241 taactctatc cgcggccatg agttcgcagg ttgtattgcg caggttggtg aaaaagtcaa
     9301 agactggaaa gtggggcaac gcgtcgtgtc ggataacagc ggtcacgtct gcggcgtttg
     9361 tccggcctgt gaacaaggtg attttctgtg ttgtacagaa aaagtaaacc ttggtctgga
     9421 taacaatacc tggggcggtg gtttttccaa atattgtctg gttcctggtg aaattctcaa
     9481 aattcatcgt catgcgttgt gggaaatccc tgatggtgtt gattatgagg acgcagccgt
     9541 acttgaccct atctgtaatg cctacaaatc catcgcgcag caatcgaaat tcctccctgg
     9601 tcaggatgtg gtcgtcatcg gcactggccc actcgggctg ttctccgtac aaatggcgcg
     9661 aattatgggg gcggtaaata tcgtcgtcgt tggtctgcaa gaagatgtgg cggtccgctt
     9721 cccggttgca aaagaactgg gtgcgacggc agtagtaaat ggttctaccg aagatgtggt
     9781 ggcgcgctgc cagcaaattt gtggcaaaga caatctggga ctggtgattg aatgctccgg
     9841 tgccaatatc gcactgaaac aagccatcga aatgctccgt ccgaacgggg aagtggtacg
     9901 cgttggaatg ggcttcaaac ctcttgattt ctcgattaat gacattaccg cctggaacaa
     9961 aagcatcatt gggcatatgg cctatgactc cacctcatgg cgtaacgcta tcaggctatt
    10021 agccagcggc gctatcaaag tcaaaccgat gatcacgcat cgtatcggcc tgtcgcaatg
    10081 gcgcgaaggg tttgatgcga tggtcgataa aaccgcaatc aaagtgatca tgacttacga
    10141 ctttgatgaa taaccactct cctgaaggaa cgttatcgca tggatcagat aacaaaaccg
    10201 cattgtggtg cacggctgga tcgcttaccg gattgccgct ggcattcatc aatgtttgcc
    10261 atcgtcgcgt ttggcttgct tgtctgctgg agtaatgccg ttggtggctt gatcctcgcg
    10321 cagctgaaag cgttgggctg gacagataat tccaccacag ccacattctc agcaatcacg
    10381 accgccggaa tgtttctcgg tgcgctggtg ggcggcatca ttggcgacaa aaccggtcgc
    10441 agaaatgcgt tcatcctcta tgaggccatt catattgcct cgatggttgt cggtgctttc
    10501 tcaccgaata tggatttcct tattgcctgc cgttttgtga tgggagttgg gctgggagct
    10561 ttactggtta cgctgtttgc tggtttcacc gaatatatgc ccggtagaaa tcgtggaacg
    10621 tggtcaagtc gggtttcctt tattggcaac tggtcatatc cgctctgttc attgatagcg
    10681 atgggactca cgccgctgat tagtgcagag tggaactggc gggtacaact gcttatccct
    10741 gcaatattgt cgcttatcgc tacggcgctg gcctggcgct actttcctga atccccgcgc
    10801 tggctggaat cgcgcggacg gtatcaggaa gccgagaaag tcatgcggag tatagaagaa
    10861 ggcgtcatac gccatacagg taaacctttg ccgcccgtgg ttattgctga tgacggtaaa
    10921 gcaccacaag cggtgccata ttcagcctta ctgacaggag tattactgaa acgcgtgata
    10981 ttaggctctt gtgtgctgat tgccatgaac gttgtgcagt acacactaat taactggttg
    11041 ccaacgatat tcatgaccca ggggattaat ttaaaagact cgattgtttt aaataccatg
    11101 agtatgtttg gcacgccatt tggcattttt attgccatgc tggtgatgga taaaattccg
    11161 cgtaaaacaa tgggtgtggg gctattaatc ctgattgcgg tgctcggata tatctattca
    11221 ctgcaaacca gcatgttgct cataacgttg attggtttct tcctgattac tttcgtctat
    11281 atgtacgttt gctatgcctc ggcagtgtat gtccctgaga tctggccgac agaggccaaa
    11341 ctccgtggct ccggtctggc gaatgcggta gggcgaatca gtggtattgc cgcaccttat
    11401 gccgttgcag tgctgctcag tagttatggc gttacgggag tctttattct tctgggggcg
    11461 gtttcaatta ttgtcgcaat tgctatcgcc accattggaa tcgaaaccaa aggtgtctcc
    11521 gttgaaagtt taagtattga tgcagtagcc aataaataat acgggtggat tcaaaatgaa
    11581 aaattcaaaa gcaatattgc aggtgccggg cacaatgaaa attatttcag cagaaatacc
    11641 agtgcctaaa gaagatgaag ttttgattaa agtagaatat gtcggtattt gtggttcaga
    11701 tgtacatggt tttgaatcag gtccgtttat tccgcctaaa gacccaaatc aagaaattgg
    11761 cctgggtcat gaatgcgccg ggacggttgt ggctgtggga agccgtgtgc gcaaatttaa
    11821 accgggggat cgggtaaata tcgaacctgg cgttccttgc ggtcactgtc gttactgtct
    11881 ggaaggcaaa tataacatct gcccggacgt tgattttatg gcgacacaac ccaactaccg
    11941 cggcgcatta acgcactatc tgtgtcatcc ggagagcttt acttacaaac tgcccgacaa
    12001 tatggacacg atggaaggga cgctggtgga gcctgccgca gtcgggatgc atgccgcgat
    12061 gctggcagat gttaaaccgg gtaagaagat aattattctg ggagcaggtt gtattggttt
    12121 gatgacgttg caagcgtgca aatgcctggg agcaacggaa attgccgtcg ttgatgtgct
    12181 ggaaaaacgt ctggcaatgg cggaacagct tggtgcgaca gtggttatta acggcgcaaa
    12241 agaagacact attgcacgct gtcagcaatt taccgaagac atgggcgcag atattgtttt
    12301 cgaaacagcg ggttctgcgg tcaccgttaa acaggcacct tatctggtaa tgcgtggcgg
    12361 taaaattatg attgttggta ctgtacccgg cgcttcggca atcaatttcc tcaaaatcaa
    12421 tcgcgaagtc actatccaga cggtattccg ctatgccaat cgttatccgg tcacgattga
    12481 agcaatttct tcagggcgat tcgatgtgaa atcgatggtg acgcatattt acgattatcg
    12541 ggatgtacaa caggcatttg aagagtcagt taacaacaaa cgcgacatta ttaaaggcgt
    12601 tattaagatt agcgattaat taatacggga gaacagaaca tgctcgcaga tatcagatat
    12661 tgggaaaacg atgccacaaa taaacgttat gctattgccc attttaatgt atggaatgca
    12721 gaaatgttga tgggcgttat cgacgcagcc gaagaagcga aatcaccggt tattatttct
    12781 tttggcacag gttttgtcgg caacacctca tttgaagatt tctctcacat gatggtatct
    12841 atggcacaaa aagcaacggt gccggtaata actcattggg atcatggtcg gagtatggag
    12901 attattcata acgcctggac tcatggtatg aattcattaa tgcgtgatgc ttccgcattt
    12961 gatttcgaag aaaatattcg tttaaccaaa gaggctgttg atttcttcca tccgctgggt
    13021 attccggtag aggcggaatt agggcatgtc ggtaatgaaa ccgtttatga ggaagcgctg
    13081 gcgggctatc actataccga tcctgaccag gcggctgaat ttgttgaacg tacgggctgt
    13141 gactcattgg ctgtcgccat cggcaaccag catggggttt atacgtcaga gccacaattg
    13201 aactttgagg tcgtcaaacg cgtacgcgat gcagtgtctg ttccgctggt tttgcatggc
    13261 gcatcgggga tcagtgatgc cgacattaaa actgcaattt cgttgggtat cgcgaaaatc
    13321 aacatccata cggagctctg tcaggccgca atggtcgcag tgaaagaaaa ccaggatcag
    13381 cctttcctgc atttagaacg tgaagtacgt aaagctgtaa aagaacgggc attggaaaaa
    13441 attaaactgt tcggttctga tggcaaagcg gaataatgat atggataatc tcgacgttat
    13501 ttgtataggt gccgctattg ttgatattcc attgcaaccg gtcagtaaaa atatctttga
    13561 tgtggattct tatcctcttg aaagaatcgc aatgaccacc gggggtgatg caataaatga
    13621 agcaacaatt atttctcgtc tgggccattg tacagcatta atgagccgta ttggtaaaga
    13681 tgccgcaggg cagtttattc tcgaccattg ccgcaaagag aatattgata ttcaaagcct
    13741 gaagcaggac gtcaacatag atacctctat taatgttgga ctggtgacgg aggatggcga
    13801 gcggacgttt gtcacaaacc gtaacggcag tctgtggaaa ttgaatattg acgatgttga
    13861 ttttgcgcgg ttttcccagg cgaaattatt atcactggcc agtattttca acagtccact
    13921 attggatggt aaagcgctaa cagaaatttt tacgcaagct aaagcccggc agatgaatat
    13981 ctgtgccgat atgattaaac cgcggttgaa tgaaacgctg gatgatattt gcgaagcatt
    14041 gagttatgtc aattatgccg aggcaaaatt actcaccggg aaagagacac tggatgaaat
    14101 tgctgacagc tttcttgcgt gcggcgtaaa aacggtggtg attaaaacgg gtaaagacgg
    14161 ctgctttatc aagcgtggtg acatgacgat gaaggtgccg gcggtcgcag gaataaccgc
    14221 catcgacacc attggcgcgg gcgataactt tgcttcaggt tttattgcgg cactgttaga
    14281 aggaaaaaat ctgcgtgaat gcgcacgctt tgccaatgca acggcggcta tctcggttct
    14341 aagcgtcggt gccaccaccg gcgtaaaaaa cagaaagctg gtcgaacaat tgctggaaga
    14401 atacgaagga taatgaaggc aaatgaaaaa gataccttta ggcacaacgg atattacgct
    14461 ttcgcgaatg gggttgggga catgggccat tggcggcggt cctgcatgga atggcgatct
    14521 cgatcggcaa atatgtattg atacgattct tgaagcccat cgttgcggca ttaacctgat
    14581 tgatactgca ccaggatata actttggcaa tagtgaagtt atcgtcggtc aggcgttaaa
    14641 aaaactgccc cgtgaacagg ttgtagtaga aaccaaatgc ggcattgtct gggaacgaaa
    14701 aggaagttta ttcaacaaag ttggcgatcg gcagttgtat aaaaaccttt ccccggaatc
    14761 tatccgcgaa gaggtagaag ccagcttgca acgtctgggt tttgattaca tcgatatcta
    14821 catgacgcac tggcagtcgg tgccgccatt ttatacgccg atcgctgaaa ctgtcgcagt
    14881 gcttaatgag ttaaaggctg aagggaaaat tcgcgctata ggcgctgcta acgtcgatgc
    14941 tgaccatatc cgcgagtatc tgcaatatgg tgaactggat attattcagg cgaaatacag
    15001 tatcctcgac cgggcaatgg aaaacgaact gctgccgcta tgtcgtgata atggcattgt
    15061 ggttcaggtt tattccccgc tagagcaggg attgttgacc ggcaccatca ctcgtgatta
    15121 cgttccgggc ggcgctcggg caaataaagt ctggttccag cgtgaaaaca tgctgaaagt
    15181 gattgatatg cttgaacagt ggcagccact ttgtgctcgt tatcagtgca caattcccac
    15241 tctggcactg gcgtggatat taaaacagag tgatttaatc tccattctta gtggggctac
    15301 tgcaccggaa caggtacgcg aaaatgtcgc ggcactgaat atcaacttat cggatgcaga
    15361 cgcaacattg atgagggaaa tggcagaggt cttggggcgt taaatactca acacgattca
    15421 cctctgtttc tgtgaaaact gtgatgaaaa tctgtgattc aaacaggtta ttttgaaagt
    15481 aaacatcggt tatgcgataa tcgcgctaat gtgatgtaaa aagatacagg ggtgtaatcg
    15541 tggcggcaaa agacaggatt caggcaatta agcaaatggt tgccaacgat aaaaaggtga
    15601 cagtctcaaa tttgagtggg atttttcagg taaccgaaga aaccattcgc cgcgatcttg
    15661 agaagctgga agatgaaggc tttttgacca gaacctatgg tggtgctgtt ttaaatacag
    15721 cgatgttgac ggagaatatc catttttata agcgcgcttc atcgttttat gaagagaagc
    15781 agcttattgc acgcaaggca ctacccttta tcgacaataa aaccaccatg gcagccgatt
    15841 ccagtagtac cgtaatggaa ttgctcaaat tgttacagga ccgtagtgac ctgacgttgc
    15901 taaccaactc ggcggaagcg attcatgttc tggctcagtc agaaattaaa gtcgtttcaa
    15961 cgggtgggga actaaacaaa aatacacttt cactgcaagg aagaattact aaagagatca
    16021 tcagccgcta tcatgtcgat atcatggtaa tgagttgtaa aggtcttgat attaacagcg
    16081 gcgcgctgga ctctaatgaa gcggaagctg aaatcaaaaa gacaatgatc cgtcaggcga
    16141 cagaagttgc gttattggtt gatcactcta agtttgatcg caaagctttt gtccagttag
    16201 ccgactttag tcatattaat tacataataa ctgataaatc accgggtgca gagtggatag
    16261 cattttgcaa agacaataat atccagctgg tgtggtaatt taagcacgcg ttgtcaatat
    16321 tatttgttcc tgcgcctgga aataaaatga tgataaagat gcggcaagtg aatttgccgc
    16381 aattgctacc ttttttagca aatccgaggc acccaatgga acaatatgat caaattggcg
    16441 caagactgga ccgcttgcct ttggcccggt ttcattatcg tatatttggt attataagct
    16501 ttagtctgtt attaac
//
LOCUS       DAZFTN010000102        16346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFTN010000102 DAZFTN010000000
VERSION     DAZFTN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            249..2945
                     /gene="stcE"
                     /locus_tag="ACHKV2_003751"
     CDS             249..2945
                     /gene="stcE"
                     /locus_tag="ACHKV2_003751"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO6941558.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            3032..3907
                     /gene="gspC"
                     /locus_tag="ACHKV2_003752"
     CDS             3032..3907
                     /gene="gspC"
                     /locus_tag="ACHKV2_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6941559.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3908..5875
                     /gene="etpD"
                     /locus_tag="ACHKV2_003753"
     CDS             3908..5875
                     /gene="etpD"
                     /locus_tag="ACHKV2_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO6941560.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5875..7380
                     /gene="gspE"
                     /locus_tag="ACHKV2_003754"
     CDS             5875..7380
                     /gene="gspE"
                     /locus_tag="ACHKV2_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO6941561.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7382..8605
                     /gene="gspF"
                     /locus_tag="ACHKV2_003755"
     CDS             7382..8605
                     /gene="gspF"
                     /locus_tag="ACHKV2_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6941562.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8636..9070
                     /gene="gspG"
                     /locus_tag="ACHKV2_003756"
     CDS             8636..9070
                     /gene="gspG"
                     /locus_tag="ACHKV2_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6941563.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            9067..9621
                     /gene="gspH"
                     /locus_tag="ACHKV2_003757"
     CDS             9067..9621
                     /gene="gspH"
                     /locus_tag="ACHKV2_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6941564.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9618..9983
                     /gene="gspI"
                     /locus_tag="ACHKV2_003758"
     CDS             9618..9983
                     /gene="gspI"
                     /locus_tag="ACHKV2_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6941565.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9980..10579
                     /gene="gspJ"
                     /locus_tag="ACHKV2_003759"
     CDS             9980..10579
                     /gene="gspJ"
                     /locus_tag="ACHKV2_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6941566.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10576..11553
                     /gene="gspK"
                     /locus_tag="ACHKV2_003760"
     CDS             10576..11553
                     /gene="gspK"
                     /locus_tag="ACHKV2_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO6941567.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11487..12764
                     /gene="gspL"
                     /locus_tag="ACHKV2_003761"
     CDS             11487..12764
                     /gene="gspL"
                     /locus_tag="ACHKV2_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6941568.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12751..13263
                     /gene="gspM"
                     /locus_tag="ACHKV2_003762"
     CDS             12751..13263
                     /gene="gspM"
                     /locus_tag="ACHKV2_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO6941569.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13384..14154
                     /locus_tag="ACHKV2_003763"
     CDS             13384..14154
                     /locus_tag="ACHKV2_003763"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO6941570.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14246..14647
                     /gene="eptO"
                     /locus_tag="ACHKV2_003764"
                     /gene_synonym="gspS"
     CDS             14246..14647
                     /gene="eptO"
                     /locus_tag="ACHKV2_003764"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO6941571.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14826..15997
                     /locus_tag="ACHKV2_003765"
     CDS             join(14826..15075,15075..15997)
                     /locus_tag="ACHKV2_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6941572.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
BASE COUNT         4050 a         3590 c         4629 g         4077 t
ORIGIN      
        1 gtttgatgtg ttaaacgtta taaacatatt aattgtcgtg ttttgtgctt gcttatcaaa
       61 aggatagtta gcttgctttt tttgcaaaat ataataataa aataagttaa aggacgttat
      121 aagagttaat tctttggtca ggatatattt acgaaacagg tgtaaatatg ttataaaaat
      181 aaccaacgac tagtgaataa gtcgctcctg aaaaaataaa atatagaaat acagttatat
      241 ccggctgcat gaacactaaa atgaatgaga gatggagaac accgatgaaa ttaaagtatc
      301 tgtcatgtac gatccttgcc cctctggcga ttggggtatt ttctgcaaca gctgctgata
      361 ataattcagc catttatttc aatacctccc agcctataaa tgatctgcag ggttcgttgg
      421 ccgcagaggt gaaatttgca caaagccaga ttttacccgc ccatcctaaa gaaggggata
      481 gtcaaccaca tctgaccagc ctgcggaaaa gtctgctgct tgtccgtccg gtgaaagctg
      541 atgataaaac acctgttcag gtggaagccc gcgatgataa taataaaatt ctcggtacgt
      601 taacccttta tcctccttca tcactaccgg atacaatcta ccatctggat ggtgttccgg
      661 aaggtggtat cgatttcaca cctcataatg gaacgaaaaa gatcattaat acggtggctg
      721 aagtaaacaa actcagtgat gccagcggga gttctattca tagccatcta acaaataatg
      781 cactggtgga gatccatact gcaaatggtc gttgggtaag agacatttat ctgccgcagg
      841 gacccgacct tgaaggtaag atggttcgct ttgtttcgtc tgcaggctat agttcaacgg
      901 ttttttatgg tgatcgaaaa gtcacactct cggtgggtaa cactcttctg ttcaaatatg
      961 taaatggtca gtggttccgc tccggtgaac tggagaataa tcgaatcact tatgctcagc
     1021 atatttggag tgctgaactg cctgcgcact ggatcgtgcc tggtttaaac ttggtgatta
     1081 aacagggcaa tctgagcggt cgcctaaatg atatcaagat tggagcaccg ggtgagctgt
     1141 tgttgcatac aattgatatc gggatgttga ccactccccg ggatcgcttt gattttgcca
     1201 aagacaaaga agcacatagg gaatatttcc agaccattcc tgtaagtcgt atgattgtta
     1261 ataattatgc gcctctacac ctaaaggaag ttatgttacc aaccggagag ttattgacag
     1321 atatggatcc aggaaatggt gggtggcata gtggtacaat gcgtcaaaga ataggtaaag
     1381 aattggtttc gcatggcatt gataatgcta actatggttt aaatagtacc gcaggcttag
     1441 gggagaatag tcatccatat gtagttgcgc aattagcggc acataatagc cgcggtaatt
     1501 atgctaatgg catccaggtt catggtggct ccggaggtgg gggaattgtt actttagatt
     1561 ccacattggg gaatgagttc agtcatgaag ttggtcataa ttatggtctt ggtcattatg
     1621 tagatggttt caagggttct gtacatcgta gtgcagaaaa taacaactca acttggggat
     1681 gggatggtga taaaaaacgg tttattccta acttttatcc gtctcaaaca aatgaaaaga
     1741 gttgtctgaa taatcagtgt caagaaccgt ttgatggaca caaatttggt tttgacgcca
     1801 tggcgggagg cagccctttc tctgctgcaa accgtttcac aatgtatact ccgaattcat
     1861 cggctatcat ccagcgtttt tttgaaaata aagctgtgtt cgatagccgt tcctccaccg
     1921 gcttcagcaa gtggaatgca gatacgcagg aaatggaacc gtatgaacac accattgacc
     1981 gtgcggagca gattacggct tcagtcaatg agctaagtga aagcaaaatg gctgagctga
     2041 tggcagagta cgctgtcgtc aaagtgcata tgtggaacgg taactggaca agaaacatct
     2101 atatccctac agcctccgca gataatagag gcagtatcct gaccatcaac catgaggccg
     2161 gttataatag ttatctgttt ataaatggtg acgaaaaggt cgtttcccag gggtataaaa
     2221 agagctttgt ttccgatggt cagttctgga aagaacgtga tgtggttgat actcgtgaag
     2281 cgcgtaagcc agagcagttt ggtgttcctg tgacgaccct ggtggggtat tacgatccgg
     2341 aaggcacgct gtcaagctac atctatcctg cgatgtatgg tgcctatggc ttcacttatt
     2401 ccgatgatag tcagaatcta tccgataacg actgccagct gcaggtggat acgaaagaag
     2461 ggcagttgcg attcagactg gctaatcacc gggctaacaa cactgtaatg aataagttcc
     2521 atattaacgt gccaacagaa agtcagccca cacaggccac attggtttgc aataacaaga
     2581 tactggatac caaatcgctc acacctgcgc cagaaggact tacctatact gtaaatgggc
     2641 aggcacttcc agcaaaagaa aacgagggat gcatcgtgtc cgtgaattca ggtaaacgtt
     2701 actgtttgcc ggttggtcaa cggtcaggat atagccttcc tgactggatt gttgggcagg
     2761 aagtctatgt cgacagcggg gctaaagcga aagtgctgct ttctgactgg gataacctgt
     2821 cctataacag gattggtgag tttgtaggta atgtgaaccc agctgatatg aaaaaagtta
     2881 aagcctggaa cggacagtat ttggacttca gtaaacctag gtcaatgagg gttgtatata
     2941 aataatcctg agacggtgcg gccacttcgg ccgcattagt aacacattcg tcccgttaat
     3001 caaaacataa agtagcgtcc agcgttttat tatgttgttc tttctatcat tccggggtga
     3061 ccggggctta tttataaaag atatcgtact taaaatgctg acgccaaacc ggctattgtg
     3121 cgtaatttta cttattgccg gatatcagct ggtgtcggtt atccatcatt tctggctgac
     3181 tcaggcagca tcggtgcccg gcttatcccg tgttagcgcg ccggaaacag cggtaactgg
     3241 tgatcaaact gaagaacgtt ttgtttttac gttattcggc agggcatccc cactatcatc
     3301 ggaggggaga gcgcaggaaa caatgccttc cctgtcagat gatctgcttt caggggagga
     3361 tcttgacgtg agaggtatac tttatagttc ggttgcagag cattccgttg ccatatttgc
     3421 acataataac agacagttca gtctgagcgt cggcgaaaaa gtacccagct acgatgctac
     3481 gattagtgct atttttagcg atcatattgt tattaactat cagggaaaaa ctgtatcact
     3541 gcctctgcga tatgataata ccgaaaaaaa gaatgcatat gacaataata atttaacagt
     3601 tggagacgtg ataacccaag ataattttcg ggtagaaagt gtttttgata ttatgagctt
     3661 ttcagccgtt acggttaata atacattaag cggttatcgc ttgattccgg gtaagcacag
     3721 ttcgttattt tataatgctg ggttgcatga taacgatctg gccgtatcgg ttaatggttc
     3781 agaattgcgt gataccagac aggcgcagca gataatgaag caattgccag aacttaaaga
     3841 aataaaaata accgtcgagc gtgatggtca gttatatgat gcatttattg ctgtaggaga
     3901 aaactgattg ttcaggaaat ggttgaatgg gcgtttgccg gtacttgtgt tcactacagt
     3961 aattttgggg gccattccag ggtggggggc tgaattttcg gccaacttta aagatacgga
     4021 tattcaggag ttcataaata ctgtcagtaa aaatttacac aaaacggtaa taattaatcc
     4081 tgacgtgcag ggaaccatca ctgtacgcag ctacgatatg ctgaacgagg aacaatatta
     4141 tcagttcttt ctcagtgtgc tggacgttta tggttttgct gtggtcgata tgcacaacgg
     4201 tatactgaaa gtagtgcgct caaaagatgc caaaacgtcg gcggtgccgg tagctagtga
     4261 tgtcagtccc gggactggtg atgaggttgt tacccgggtg gtccccgtaa gtaacgtggc
     4321 agccagagat ctggcgcctt tgctgcgtca gctcaatgat aatgctggcg caggaagcgt
     4381 ggtgcattat gaaccttcta atgttttgtt gatgaccgga cgtgctgcag tgatgaaacg
     4441 gttgatggag attgttgaac gtgtggataa ggtgggtaat cgcagcgttg ccacggtccc
     4501 gctcacctac gcgtccgcaa cagacgtagc cagacttgtt acggaactga ctaaagaaac
     4561 agataagaca gctatacctg cttggatgac ggcgaaactg gttgcagacg agaggacaaa
     4621 ctcagtgctc gtcagcggag agccaatctc ccaacagcgt atcatctcca taattaagca
     4681 actggatcgt caggaggatg ttcagggtaa tactaaggtg atttacctga aatatgcgaa
     4741 ggcgaaggat ttagtggaag tcctgacagg tatcagcagc agtattgaaa acgactctaa
     4801 aaagagtccg tcaacggaag ccttgcgcaa aggagtgacg attaaatccc acgaacaaac
     4861 caatgccctg atcctgacgg gggcccctga cgtcatccgc gaccttgaaa atgtgatttc
     4921 gcagttggat attcgtcgtc ctcaggtcct ggtggaggcc atcattgctg aaatacagga
     4981 tgctgacggg ctgaaccttg ggatccagtg ggtgaataaa catgccggtg tggcgcagtt
     5041 caccagtacc ggtttaccta ttaccacgat ggttcagact cgtcagaacg aaatcttaga
     5101 cagcgatcag agcaatgccc tgagcatgtt taacggaatt gcagcggggt tttatcaggg
     5161 aaactgggcg atgctgttga cggcgctctc cacaagtagc aagaatgata tcttggcgac
     5221 ccccagtatt gtcacgctgg acaatatgga ggccactttc aatgttggtc aggaggtccc
     5281 ggtactttcg ggctcacaga caacctctgg ggacaatatt tttaacacgg tcgagcgcaa
     5341 aacggtgggg atcaaactca gggtaaaacc ccagatcaac gagggtgatt ccgtgttact
     5401 ggagatagaa caggaggtgt ccggtgtggc ggacactgca gtagccacca ctactgactt
     5461 gggagcaacc ttcaacaccc gaacagtgac caatgccatg ctggtcggga atggcgaaac
     5521 ggtggtggtc ggaggattac tggataagtc gatcaggggg agtgagagta aagtgccact
     5581 gctgggggat atcccggtac tggggcatct ttttcgcgca aaaagcgaac agacagctaa
     5641 gcgtaatctg atgctgttca ttcggccaac tattattcgt gagcgcgacg gatttcgtca
     5701 tgcttcggcc gaaaaatacc agtcgtttaa tcaggaacag gtgcagtcgc gtggcaaaga
     5761 aacaacggcg ctgacgctga atgaggaaca gctcaggctg tcccccgatc aagacgatac
     5821 ggctttccgg aaggtgaaag cggcgattgc tgcgttttat gcgcaggaga tgtaatgagc
     5881 agagtagtac agaatgtcag tgaatcacgt ccccttctgc ctttttcctt ttcccgtacg
     5941 cagagaattt tgttgctgcg tgaacaggag gggaatcgag tgttctgcat ggaggacaca
     6001 cctgccagtg ccctgttgga ggtgcgcagg gtggctgaag gtcctctgaa tgtcacaaca
     6061 gtgtcggcgg aagcgtttga aaaacagctg gtgagcagct atcaacgtga ttctgatgaa
     6121 gcgcgacaaa tgatggctga gattggtaat gaaatggatt tttataccgt cgcaggggag
     6181 ttgcctgacc gtgaggactt actggatgcg aatgacgatg cgccaattat ccgcctgatt
     6241 aacgcaatgc tgacagaggc aattaaagag aaagcctcag atattcatat tgaaacttac
     6301 gaacgccatc tgcaggttcg ctttcgcatt gatggcgttc tgcgggagat cctccgttta
     6361 cataggaatc tggcttcgtt actgatttca cgcattaaag tcatggcccg gttggatatt
     6421 gccgaaaaac gcgttcccca agatggccgc atggtactgc gtatcggtgg tcgggcggtg
     6481 gatgtgcgtg tttcaacgtt gccttcaaat catggtgaac gcatcgtgct gcgtttacta
     6541 gacaaaaata gcgttagtct cgatcttgct gcactcggca tgtcgcagca gaatcagcga
     6601 cacattgatg cactgatccg ccgtcctcac ggcattattt tggtgaccgg gcctacaggg
     6661 tcgggtaaaa gcacgacgct ttatgccgcg ttaagcctgc tgaatccccg agaccgcaac
     6721 attatgacgg tcgaggatcc tgttgaatat gaactggacg gtatcagtca gacccaggtc
     6781 aacccgaagg tggacatgac ctttgcccgg agtctgcgcg ctattttacg tcaggatccg
     6841 gacgtggtgc ttgtggggga gatccgtgac ggtgaaacag cccagattgc tgtgcaggcc
     6901 tcgctgacag ggcatttggt gctgtcaacg ttacatacga atagtgcagc aggcgcgctg
     6961 tcgcgtctgc aggatatggg catccctcct tttctgcttt ccacctcatt actggctgtt
     7021 ctggcccagc gactggttcg cacgttgtgt ccgcgctgtc gtcagccctg tcaggtcagt
     7081 actgagttag cgatggacat ggacattccc cctgaaacca cgatctggca gcctgccggg
     7141 tgtcagcact gcagtttcac gggctatcac gggcgcaccg ggatccacga gctgttgctg
     7201 attgacgatc gcattcggac ggcaatctat cagggggagg gggagctggg tattacccgc
     7261 ttggcaggga gtcgctatct gacgctgcgt ggcgacgggc gacaaaaggt actagccggt
     7321 gagaccagtt gggaggaggt ggttcgcgtt actgaaagca gattgcagga agaggaatga
     7381 tatggcgttg tttcattatc aggcatcaga tattcacggt agaaaacgca gtggcattct
     7441 ggaggcggat tcggcccgac atgcccgtca gttgctgcgt gagcaggcgc ttattccagt
     7501 caggttggat gagaaacagg tccatcacaa gcactcactg cggagtatcc tgaggtttcg
     7561 tccgcgcggg gggagcagcg ccgaactcgc gttactgaca cggcagttag ccacactagt
     7621 ggccgcatct ctgccgcttg aagaggcgct ggatgcgttg ttgaggcaga gtgaaaaacc
     7681 gcgtcagcgg aacttaatcg ctgccgtgcg caccaaggtt ctcgagggac attccttggc
     7741 cgcggcgatg ggcatgtttc caggtacgtt tgagcgtctt tattgtgcga tggtggcggc
     7801 gggggaaaca tccggtcgtt tggacgtggt gctcagtcgt ctggctgact ataccgagca
     7861 gcgccagatc atgcgaaacc gtctactcca ggcattgctc tatccttgtg ttctgacgtt
     7921 ggtggcggtt ggggttattg ccattctgct tactgcggtt gtgccgaaag tggttgagca
     7981 gtttattcat atgaaacaga cccttccctt atctacccgc gtactgatgg gggctgctga
     8041 ggtgagccag acttggggcc cgtggctgtt actggccgca gcgctgggcg ggatagcggg
     8101 gcgcatgata ttacatcagc cctcccagcg tctggctttt catcatctgc tgttgcggct
     8161 gccggtcgtg gggcgcatat cgcgcggtct gaataccgca cgttatgccc gcacgctgag
     8221 cattcttaat gccagtgcgg taccgctgct ccaggcgatg cacatcagtg gtgacgtact
     8281 gagtaatgac tgggcccgtc atcagttagc taccgcggcc gagttggtta gggaaggggt
     8341 cagcctgcat caggcactgg agcagacttc gctgtttccg cctatgatgc ggcacatgat
     8401 tgccagcggt gaaaatagtg gcgaactcga cagcatgttg gaacgggccg ccgacaatca
     8461 ggatcgtgag ttcagcacac agatgcaact ggcgctggga ttgtttgaac cgctgttggt
     8521 ggtgggtatg gccggggtcg ttttgtttat tgttctggca attctgcagc cgctcctgca
     8581 gctcaacaac atgatgaata tgtgaatcct tctttaacag tcaacggaga aaaacatgcg
     8641 aaaacagcat cagcgtggtt ttaccttgct ggaaataatg gtggtaattg tgatcttggg
     8701 agttttggcc agtctggtgg tcccgaatct gatgggaaat aaggataagg ccgatcggca
     8761 gaaagtgatg agcgacttgg ttgcgctgga aagcacgctc gatatgtacc ggttagataa
     8821 taaccggtat ccgacaacgg agcagggact gcgggcactg gtcagtaaac ccaccgtgca
     8881 acctgaaccc cgtaactacc ggcaggatgg ttatatccgc cgtttgcccc aggatccttg
     8941 ggggggggat tatcagctgt taaatcctgg tcagtacagc gacattgata tcttttctcc
     9001 tggcccggat ggtgtgccaa acactgaaga tgatattggt aactggacgc tcggtaacgc
     9061 gcagccatga gtcagcgcgg tttcacaata ctggagatga tgctgatcct tttgttgatg
     9121 ggcatcactg cagggctggt actaatgtca tttcctgact ctgctcagaa tcatctgcaa
     9181 caacagcggg agcgtctgca ggcgcagctg gattatgcct tggatagaag ccagcaggac
     9241 ggcttgctga tggggattca ggttcggttt gacggatgga aatttaaggt tctgcagcgc
     9301 ggaacagcgg aaagcccccc gacattagct gagggagggg atatttggca ggggtatgtc
     9361 tggcaaactt ggcaaccgcg acgtgcagcc atggggggca aacttccgga tgatgtgcgt
     9421 cttgagctgc agtatctcag aggtttgcag tggatgtctg agcatgatga cggggctgaa
     9481 cccgatattt tgctgcttcc cggaggtgag gtaacgccgt tcagacttct tttcaggcag
     9541 gtcggagagg aggccgttgt cgggctgcag gttgatgaga acggtttgat gactcttttt
     9601 gaaggtgagg tctcattgtg aaggggcaag aaggaatgac attactggaa gtcatagtag
     9661 ctctggttgt gtttgcgctg gcaggaatgg cgctgatgca ggcctcgacc cagcaggctg
     9721 cggggatcgg acggatggaa gagaaggtgc tggcaggctg gttggccgat aaccagatgg
     9781 tgcaattgca actcgagaaa acttggccgg aaaatggctg gggcgagaag acgatcagct
     9841 tcgctggcac cgaatggtat ttacgctggg gcgggccgga cagcgacgtg ccgcagccgc
     9901 gctccttaga ggtagaggta cggcgcacta aagaggagac gtccgccttg gtttccttgc
     9961 gtagcagtgt ggttcgtgaa tgagtcagca acgggtaaaa ggttttacgc tactggaaat
    10021 gctgttggca ctggctgttt ttgcagcgtt gagcatcagc gcatttcagg tgctgcaggg
    10081 tggaatacgg gcgcacgagc tgtcccggga taaagtacag cgtctggctg agctgcagag
    10141 agggatcagc cagatggagc gagatcttac gcagatgctc ccaaggcaca gccgggggaa
    10201 tgaaggcctg ctgctcgctg cacctcatct gctaaaaagt gatgactggg ggatttcttt
    10261 tacgcgaaac agctggctga atcccgccgg gatgctgccg cgcccggagt tacagtgggt
    10321 aggttatcgg ttgcgacagc aaaagttaga acgcctgagc tactttcatg tcgatcaccc
    10381 ttccggtgtt tctccggatg tcagggtgat gctggacggc gttcacgcat ttcgtctgcg
    10441 tttttttgtt aacggtgatt ggcaggcgcg ctgggacagt accggcatcc tgccgcaggc
    10501 tgttgaagtg acgctggtta tggatgattt tgctgaactg ccccgtctgt ttcttgtgag
    10561 caaggagacc gctgaatgaa attgcgggaa cagggcgtgg cgcttctggt ggtattgctg
    10621 atcctgtcgt taatggtaac gatagcggcg gtgattgcgg agcgcaacgg tcggacattt
    10681 ttgcgtacag tagcgcagtt ggatcagctg caggcaaagt gggatggcta tacggcagag
    10741 acgatagcaa aacaaatctt acagaggagc aggcaggagt cgccccggaa aacgcatctg
    10801 gcacaaaatt gggcgcagtc agaacgacag tttgagacca gagggggaga tgtgcggggg
    10861 cagatcgtgg atgcgcaggc ctgttttaat cttaatgcta ttaattatgg agtggtggat
    10921 ctgaccagta taccttatgc cgccagaatt tttcagcagc tattaatcaa cttgcaggta
    10981 gagcttttac aggcccggca agtgacggca gccctacgtg actggatcga tcgtgatgat
    11041 aagccggtaa ggggcggtgc tgaagacgag gtgtatatgg ggatggaacc tccctatctg
    11101 gcagccaacc agccgatgca ggacgtcagt gagttacgcc ttatccgggg aatagatgcc
    11161 aggctttacc gcaaactttt gccgtatgtt tgtgtactgc ctacgtcaga tctgtctgtg
    11221 aatgtgaata ctctgctgga ttcacaggcg ccgctgctgg cggcgctctt tctgacgaaa
    11281 ccggacagtc ttccggtgac ggaattactg cagcggcgac cccgtactgg ttgggagagc
    11341 gtggcggcgt ttcttgacgc gccaccgctt aaggacattg acaccagtgc ggccatgccg
    11401 gtattggccg tcagcagcaa ctattttctg gttcggttac atgtgcgctc cggggaacac
    11461 ctgttcagtc agcagacttt aatgcagtgg agggaggagc gtttccggat cattcagcga
    11521 cagtatggat taacgatgag ggaggttccc tgaaaaaaaa taacaaacat gtcgaggctg
    11581 gtcggctttt tctgcgactg ccggaggatg actcggagcc tttattgtgg aggctgattg
    11641 cgccagagga gacaggagcc ggcagtgtgg cagtcggaga aacggatccg cagttagtcg
    11701 gtttgctgtc ccgttatccc gcctgggtgc tggttccgtc aggcagaatt gccttccatc
    11761 aggtcacgct gccacaccgc acgtggcgtc agcgtttgca ggtgctgccc tttcttctgg
    11821 aagagcagtt ggcgacagac attgagcagc tgcacttcgc cattcttcat cagtcgggtg
    11881 acaggtgtga tgtggcggtg gtcgaaaaag cggttatgca tcgctggtta gcgtactgtg
    11941 gacaacttgg ggttcgggag gtgaggttgc tgcctgacgt actgatgctg ccgttggcaa
    12001 ctgaaggatg gagtgccgta aagcttgccg ggcagtggct gtttcgccgg gatcgttacg
    12061 ccgggatgac ggtagagtta tcgtggctga cacatttctt gtctcttact gcgccgccgg
    12121 tgattgagtc ctactccgtg ccgccagtac cgtgcccggg tccgaagacc actgagtggc
    12181 gaaatcagcc cggacgcgat ctgctgcagt tggtggccga aggtgacggg tatgatggcg
    12241 cagatctgcg gcagggagaa tttgcccgcc agggaacatg gtatgcacga tttcgcccat
    12301 ggaggtatgt cgcaggggct ctgctggcct gtgtttttct ggccgggagc aacgcggggc
    12361 tggcacatta tcggctctgg cagcaggctc aattctggcg gtatgagagc gtacgtgtct
    12421 atcagcagct gtttccgagt gaaaaggcgg tgaaggatcc ccgccggcag atgctgagac
    12481 atctgcaaca gtcgacgggt aacaatatac cggaactcgg gagcgtgatg cgccagcttc
    12541 agcaactgct gtcagaaacc tcaacgatac gcttacaggc actcgcctgg gacagcagca
    12601 gccagacact gaaaattgat ttgcaggcgg cttcttttca ggcgctggaa cactttcagc
    12661 agatcgccgg agaaaaatac ctcatccagc ctggtgagat acgacagcag ccgcaggggg
    12721 tagaaagtcg tctgatcctg agagtgaaca atgaacgagc ttaaaaaacg tttgcagtat
    12781 gttagccccc gtgagcgcag gctgcttatc ggatgtgttg ccttactggt tgttgttttt
    12841 gtctattacg tcctctggca gccctggctg atacgagagg ataagtggcg gactgtagtt
    12901 acccgtgaaa aaaacacagt ggagtggatg aaacaacagg tacccaacat aaagagacgg
    12961 gaggcaagta tgcctgaaca gggggagccg ttaggtttat ctgcggccgt gacacgtact
    13021 tcagccgcat acggtgtatc ggtcacccgg ttgcagcctc aaggggagcg tctggccgtc
    13081 accttagccc cggttgaatt taatgcgctg atgcagtggc ttacgcaact ggagcgtcgg
    13141 caccgtattc gtaccatggt atttgatgtg gccgcgcagg gcaacccacc cggacaggta
    13201 acggttaatc ggctggtttt gtcccaagat gacgtaaaca aggacgcttc gcttagtcgc
    13261 tgatgtcgta atttacagat taatgcttga tgtaaaaatt ggacttataa aggaggatgt
    13321 gtgagtagtt tgccggaatt tgcggttgat tatcccttgt tgtggctcat ctgcaccgga
    13381 gggctggggg gcgttaccgg cagttttctt aacgtcgtga ttcatcgtct gccgataatg
    13441 ttggagaggc ggtggcggcg tgaagcgctg acgctattgg cacattctgt gcctgagtcc
    13501 gggccggcct ttaacctgat gataccgcag tctcattgtc cctgctgttt tcatccgctc
    13561 ggcatcaggg ataacattcc cctgttaagc tacctatttc tgaaggggaa atcacgctgt
    13621 tgtggcgagc gtattcccgt ttactacccg ctagtcgaaa taacgaactc agtgttattc
    13681 attctggccg cgagcaggtt tccaccaggg ctgacattag cggctgcctg gctttttatt
    13741 tccatgctgt tggttctggc ggttattgac tgccatacgg cgctgctgcc ggatgtgctg
    13801 acgttgccat tgctctggct tggcctgtta ttcagtttac agagaggcgt tgtcacctta
    13861 gaggaggcgg tagtgggggc ggtatctggg tatctctgtc tgtggggact gtactggctt
    13921 tttcgtttcg ctaccgggag ggaagggtta ggttacggtg attttaagct agctgcggca
    13981 ttgggagcat ggatgggttg gcaggcatta ccctcaatac ttttgtttgc ctcagtcagt
    14041 gggttaatcg taacagtact gttgcgtatt gtaacagcta cggactttac ccgcccgctg
    14101 ccttttgggc cgtggctcgc tttgagcgga gtgtgccatt ttttgttgat gtagcgttcg
    14161 gttaccgggg ctagtaataa aaacggctca gcggataaaa gaactgttgt aaaccggtat
    14221 caggttaaac gatacctagt aaaaaatgat gggtaatatt ttgaaaaaac tgaattgtat
    14281 cgcgtcatta ctagtcttag tgactatctc tggttgtcat cagtcaccct ccatccataa
    14341 gcaggccact gttcctccga gcgaacaact ggaacaaatg gcgtcgattg tctctgcgac
    14401 acggtatctg aaaatgagat gtaaccgtag tgatttacca gatgaacagt cgatcctaaa
    14461 tgtcgctaac cggatcgcta tcgggaaagg gtggcagtca ttaactcagg aggatatacg
    14521 taaacacagc gatgatatct atgtgcgtct gactagggac agtacgcctg agtatataaa
    14581 atgcagagaa ttcaatcggc ggctggtccc atttatcggt gaattattgg caaggggtcg
    14641 tggataatat gaaccgcccc ggaaatcctg gagactaaac tccctgagaa agaggtaaac
    14701 agcatgaata aaaatgggtc ttcccttgta gtggtggctg aaggcatgat gattgtagtg
    14761 gcacactgaa tttggccacc tgaacagagg tgatatgctc acctcagaac aacacaggtg
    14821 ctccaatgaa aaaaagaaat ttcagcgcag agtttaaacg cgaatccgct caactggttg
    14881 ttgaccagaa ctacacggtg gcagatgccg ccaaagctat ggatatcggc ctttccacaa
    14941 tgacaagatg ggtcaaacaa ctgcgtgatg agcgtcaggg caaaacacca aaagcctctc
    15001 cgataacacc agaacaaatc gaaatacgtg agctgaggaa aaagctacaa cgcattgaaa
    15061 tggagaatga aatattaaaa aaggctacaa ccgcgctctt gatgtcagac tccctgaaca
    15121 gttctcgata atcgggaaac tcagagcgca ttatcctgtg gtcacactct gccatgtgtt
    15181 cggggttcat cgcagcagct acagatactg gaaaaaccgt cctgaaaaac cagacggcag
    15241 acgggctgta ttacgcagtc aggtacttga gttgcataac atcagccatg gttctgccgg
    15301 ggcaagaagc atcgccacaa tggcaaccct gagaggcttc agaatggggc gctggcttgc
    15361 cggcaggctc atgaaagaac tgggactggt cagttgccag cagcctgcgc accgttataa
    15421 acgaggtggt cgtgaacatg tcactatccc gaatcacctt gggcggcagt tcgcagtgac
    15481 agagccaaat caggtatggt gcggcgacgt gacgtacatc tggacgggga aacgttgggc
    15541 ataccttgcc gttgttctcg acctgtttgc aaggaaaccg gtaggttggg caatgtcgtt
    15601 ctctccggac agcagactga ccatcaaagc gctgaaaatg gcctgggaaa tccgcagtaa
    15661 accagccggg gtaatgttcc acagcgatca gggcagccac tatacaagca ggcagttccg
    15721 gcagttactg tggcgttacc agatcaaaca gagtctgagt cgacgaggaa attgctggga
    15781 taacagcccg atggagcgct tcttcaggag tctgaaaaac gagtggatac cggtgacggg
    15841 ttacatgaac ttcagcgatg ctgcccatga aataacggac tatatcgttg ggtattacaa
    15901 cgcgctcagg ccgcacgaat ataacggtgg gttgccacca aatgaatcgg aaaaccgata
    15961 ctggaaaaac tctaaagcgg tggccagttt ttgttgacca ctacatcccg tcagccgatt
    16021 tacgggccgt aagtaacgaa gttggatgca aatgtcagat cgtgtgcgcc tgttatggct
    16081 cggctggtaa gagagcctta taggcgcatt tgaaaaaact ccagctatac cggaagagat
    16141 ttattattaa caggagaggt ttgcttttct gtgtaggttt agattttgtg tttgcagggt
    16201 aaacacaact gattaactcg tcatttcggt aactgaaata gtttgctgta ttctgtttac
    16261 aatctcttta ataaggcatt tgtcacgtgg ctattcatat gaaaatcata cgttatctta
    16321 caaatcaatc atctgagtgt tataat
//
LOCUS       DAZFTN010000103        16330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFTN010000103 DAZFTN010000000
VERSION     DAZFTN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(481..906)
                     /gene="arsC"
                     /locus_tag="ACHKV2_003766"
     CDS             complement(481..906)
                     /gene="arsC"
                     /locus_tag="ACHKV2_003766"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO6941573.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(919..2208)
                     /gene="arsB"
                     /locus_tag="ACHKV2_003767"
     CDS             complement(919..2208)
                     /gene="arsB"
                     /locus_tag="ACHKV2_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO6941574.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2262..2615)
                     /gene="arsR"
                     /locus_tag="ACHKV2_003768"
     CDS             complement(2262..2615)
                     /gene="arsR"
                     /locus_tag="ACHKV2_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO6941575.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3359..3442
                     /gene="dinQ"
                     /locus_tag="ACHKV2_003769"
     CDS             3359..3442
                     /gene="dinQ"
                     /locus_tag="ACHKV2_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO6941576.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3496..4848)
                     /gene="gorA"
                     /locus_tag="ACHKV2_003770"
     CDS             complement(3496..4848)
                     /gene="gorA"
                     /locus_tag="ACHKV2_003770"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO6941577.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4920..5762)
                     /gene="rlmJ"
                     /locus_tag="ACHKV2_003771"
     CDS             complement(4920..5762)
                     /gene="rlmJ"
                     /locus_tag="ACHKV2_003771"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO6941578.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5965..8007
                     /gene="prlC"
                     /locus_tag="ACHKV2_003772"
     CDS             5965..8007
                     /gene="prlC"
                     /locus_tag="ACHKV2_003772"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO6941579.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8015..8767
                     /gene="rsmJ"
                     /locus_tag="ACHKV2_003773"
     CDS             8015..8767
                     /gene="rsmJ"
                     /locus_tag="ACHKV2_003773"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO6941580.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8816..10285)
                     /gene="dtpB"
                     /locus_tag="ACHKV2_003774"
     CDS             complement(8816..10285)
                     /gene="dtpB"
                     /locus_tag="ACHKV2_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO6941581.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10451..10504
                     /locus_tag="ACHKV2_003775"
     CDS             10451..10504
                     /locus_tag="ACHKV2_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941582.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10482..10535
                     /locus_tag="ACHKV2_003776"
     CDS             10482..10535
                     /locus_tag="ACHKV2_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941583.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10602..11036)
                     /gene="uspA"
                     /locus_tag="ACHKV2_003777"
     CDS             complement(10602..11036)
                     /gene="uspA"
                     /locus_tag="ACHKV2_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO6941584.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11427..11762
                     /gene="uspB"
                     /locus_tag="ACHKV2_003778"
     CDS             11427..11762
                     /gene="uspB"
                     /locus_tag="ACHKV2_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO6941585.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11833..13332)
                     /gene="pitA"
                     /locus_tag="ACHKV2_003779"
     CDS             complement(11833..13332)
                     /gene="pitA"
                     /locus_tag="ACHKV2_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO6941586.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13564..14766
                     /gene="yhiN"
                     /locus_tag="ACHKV2_003780"
     CDS             13564..14766
                     /gene="yhiN"
                     /locus_tag="ACHKV2_003780"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO6941587.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(15084..16136)
                     /gene="yhiM"
                     /locus_tag="ACHKV2_003781"
     CDS             complement(15084..16136)
                     /gene="yhiM"
                     /locus_tag="ACHKV2_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO6941588.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4231 a         4097 c         4231 g         3771 t
ORIGIN      
        1 ggtaatattc gtgtataaaa aagggcattg atagtatcgt aaaggaaaac gttaacaact
       61 atcaaaaagc gctcatttta aaaagttatg atgttctccg atagtgtgtg aaatgatatt
      121 tttgtgatgt tttagatgtg taataataaa atgggttaag gtatcttata attattgaat
      181 aatttgttgt gtaaagcact gataaatcat aatccagtac gtttttaaac tgctgcactg
      241 tatcgctgat cactaagcag ctcactgata catcatgctg gacgcgataa ttggaatctc
      301 acgcagggag taatgtgtaa atagcaaaat cgcctgatgc gctacgcctg tcaggcctac
      361 aaataacata caatttattg aattttcatg actttgtaga tcggataaga cgcgttagcg
      421 tcgcatccga cataaataaa gcgcactttt ctaacaacct gtggggggga tatagccgct
      481 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggtgaatgc
      541 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      601 tcccagcgtc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      661 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      721 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      781 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      841 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      901 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      961 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
     1021 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
     1081 ggtgattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
     1141 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
     1201 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
     1261 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
     1321 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggcgcacc
     1381 gcgcagcact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcaa
     1441 cgcgcccact gccgcaatgg cgctgaccgg aatgccgagc ggttccagga cgaaaaatcc
     1501 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttaatcgc
     1561 ttctgcggga gatttcagca gcgccatgtc gtagttctgc ggaatatctt tgcggaaata
     1621 gagatgcaac atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1681 ggcatattcg cgaaagccga ggccaaagaa atctgcggaa acgatattca ccaggttgga
     1741 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca caaacgccag
     1801 cgtagtgcct ttactgaacc ctaaagccag cagcatggcg atgacaatcg gtgtcaaaat
     1861 aagcgccgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca ggacaatcca
     1921 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagtg ccgcccattc
     1981 aaaaaagccg gactcatcca gcagcaggct gatgataata acggcaataa acgcagccgt
     2041 cgcgttccag acgatattcc acaccaccgg aatatcgccc ggatgtacta cgcccgtaac
     2101 taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     2161 gataaccaat acaatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     2221 aaaaacacat gtgcaaatcc atatgtgttt agataaattt tttaactaca agtgttctta
     2281 ctgtctacgg aacaattttg tctggccagg ttgcggacaa tcacctgaac cttttcctgt
     2341 tcgcatcgcc aggcctgctc aataattttc gcggcccatg atggaatatg cggtgataag
     2401 cggtaatgaa cccacttacc ctgcttgcga tccagcaaca aaccgctttc acgaagaagc
     2461 gccagatggc gggagatctt gggctgcgac tggtcgagag cagtgcagag atcacagacg
     2521 cataactctc ccagctcgct gagcagtaaa acgatgccca gacgggtttc atcggcaaga
     2581 attttgaaca gttggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2641 gtgtctctct tcgaagcgga taagtcaaaa acatatatgg tttaacgaat gtgtatgtgc
     2701 agaggaaggt aataggtgtg aattaagcgt tggctattca tttgaaagga ggtctgaatc
     2761 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagcccaga gttggtggct
     2821 ctggctggag tgagaatgcc cacatctaac ggttcaatac tattagccag tggctaacat
     2881 tgcaagcgtt agcgatttct tcagctggag aagggacagt ttgctataca tgttggtgaa
     2941 agaggattta tatactttca tcgggtcgct gccgtaccag gctttgtccg aatccggatg
     3001 taaaaaaacc gacttcgcgt cggttttttt actcttcagc cctgagttgg tggctctggc
     3061 tggagtgaga atgccatcat ctaacgcatc aatactaata ccatactgaa actattgcaa
     3121 ggacatgctg gttttataac ctgaatatac tgtatgatta tccagttagc tctgaggcat
     3181 tttcactctg gcaatgcgca taaacgcttt caaagtcctg ttcagtggta cgggtggtgc
     3241 cgttaactga tgctctggcc ggagtgagaa taccattatc taacaatagg gcatgcgccg
     3301 tgacaggcga tggatgagta agcggatgca ttctcactcc atcgtatgga gaacacgggt
     3361 gattgataaa gcaatcatcg ttcttggggc gttaattgcg ctgctggaac tgatccgctt
     3421 tctgcttcag cttctgaact gatagcggaa acgtaattaa gggcaaagag cacaccactc
     3481 tttgcccttt aacatttaac gcatggtcac gaactcttct gccgccgttg ggtgaatggc
     3541 gacggtatta tcgaagtctt ttttggttgc gcccatcttc agtgccaccg cgaagccctg
     3601 caacatttcg tccataccaa aaccaatgcc gtgaataccg acaatcttct cttctggccc
     3661 aacgcacacc agcttcatgc ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc
     3721 ggtgaaagag gatttataca ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg
     3781 ttccgttaaa ccaacagtac caatcggcgg atggctgaag accacggtcg gaatgttgct
     3841 gtaatccaga tgctcatccg gcttgttatt aaacaggcgt tcagagagac ggcgacccgc
     3901 tgcaactgcc accggtgtca gttctactgc acccgtgtta tcgcccaccg cgtaaatacc
     3961 ttcaacgttg gtgttttgat atttatcgac gacgatatag cctttttcgt tggttttaac
     4021 gccagcggct tcgaggttga tgttgtcatt ggcaggctcg cggccaatcg cccaaatcag
     4081 gcaatccacc gtttcactgc gaccatcttc cagctccagc gtcaggctac catcggcatt
     4141 tttcactacc gctttcggga tggcgttggt gtgcaactgc gggccttcgg cgttcatcac
     4201 ttcgaccagc gtttcagaga tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac
     4261 aaacagatgc gttttcgcgc cgaggccgtt aatcacgcca gccagctcaa cggcgatgta
     4321 acccgcgcca acaaccgcca cacgctctgg caaagcagga agggcaaaga aaccatcaga
     4381 atcaataccg tattccacgc ccggaatatc cgggtggctc ggacgaccgc cggtggcgat
     4441 cagaatatga tcggctgtga tggtttcacc gttaacttcc agcgttttgg catcaacgaa
     4501 gcgggcaaag cctttgatta catcaacgtt atttttaccg agcacgtttt cataggaagt
     4561 atgaatacgg tcgatatagg cggtacggct ggcgatcaac gtttcccagt tgaatttatt
     4621 gatagtggta tcaaaaccat aatccgggcc gtacatatgg atcgcttcac ggatttgcgc
     4681 cgcgtgccac atcactttct tcggtacgca gccgacgttc acgcaggtgc cgcccagctc
     4741 tttggcttca atcagcgcgc atttctgacc gtacatagcc gcgcggttga tggaggcgat
     4801 accgccgctg ccgccgccga tggcgatgta atcatagtgt ttagtcatga cagagtgtcc
     4861 ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat
     4921 tactctggca cgatccagct tacggtggcg tgcccggtgc cagctggaac cagtttgctg
     4981 tgcagccacg gcagcacgtt attcatctgc tgttccagtt tccacggcgg gttaatcaca
     5041 atcatgccgg aagcggtcat gccacggcga tcgctgtctg gcagtaccgc cagttcaatt
     5101 tgcagaattt tgcgaatgcc ggtcgcttcc aggtcgtgga tcatgcgctt aatttgctgg
     5161 cgcagcacca ccggatacca caatgcgtaa gtgccagtgg cgaaacgttt gtaaccttct
     5221 gctatcccgc tgactaccgc ctgatagtcg gttttcattt catacggcgg atcgataagg
     5281 attaaaccac ggcgagaaac cggcggcagt ttggccttaa gctgctggaa accgtcggat
     5341 ttttcgacgc gcgcacggct gtctttctga aattcagaac gcagcaacgg gtaatcgctc
     5401 gggtgcagtt cggtcagttg caggctgtcc tgttcacgca gtaactgacg ggcaatcaac
     5461 ggtgaaccgg ggtagtaacg caactgaccg ctacggttga agtgttttac cacattgatg
     5521 tacggctcca gctccgcagg caaatcgtcc tgctgccaga tacgggcgat accttcgaga
     5581 tattcgccgg tacgctcggc atgttcgctg cctaactgat aacgcccggc ccctgcgtgg
     5641 gtgtcgagat agagaaacgg tttatctttc tctttcagcg actcgatgat caggctctga
     5701 acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc
     5761 atgggtaaag gtgttccggt aagtaaaaat cggcccgtat tcggggcgca caaaagccta
     5821 tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat
     5881 tcactacact taaccccatg ctacaaacat tatgtaaagc gcctgttgag cgcttcctta
     5941 acctctttaa ccaggactgc gctaatgacg aatccgttac tgactccctt tgaattgcct
     6001 ccgttttcta aaattctccc ggaacatgtc gttccagccg tgactaaggc attgaatgac
     6061 tgccgcgaaa atgtggagcg cgtagtagcg caaggagcac cgtacacctg ggaaaatctc
     6121 tgccagccgt tggcggaagt ggacgatgtg ttggggcgta ttttctcccc ggtcagccac
     6181 ctgaactcgg tgaaaaatag cccggaattg cgcgaagcgt acgaacaaac tctgccgctg
     6241 ctgtcggaat acagcacctg ggtagggcaa catgaagggc tgtataaagc gtaccgcgac
     6301 ctgcgcgatg gcgatcatta cgccacgctg aacacggctc agaaaaaagc ggttgataac
     6361 gcactgcgcg attttgaact ctcaggcatc ggtctgccga aagagaaaca acagcgttac
     6421 ggcgaaattg ccactcgtct ttctgaactg ggcaaccagt acagcaacaa cgtccttgat
     6481 gcgacgatgg gctggactaa actcgttacc gacgaagcgg aactggcggg gatgccagaa
     6541 agcgcgctgg ctgcggcaaa agcccaggcc gaagcgaaag agctggaagg ctacctgttg
     6601 acgctggata tcccaagcta cttgccggta atgacctact gcgacaacca ggtcttgcgt
     6661 gaagagatgt atcgtgctta cagcacccgc gcttcagatc aaggtccaaa cgccggtaaa
     6721 tgggataaca gtaaggtgat ggaagagatc ctcgcgctgc gtcacgaact ggcgcaactg
     6781 ctgggctttg aaaactacgc gtttaaatca ctggctacca aaatggcgga aaacccgcag
     6841 caggtgcttg atttcttaac cgatctggca aaacgcgcgc gtccgcaggg cgaaaaagag
     6901 ctggcgcaac tgcgtgcctt cgccaaagcc gaatttggcg tggatgagtt gcaaccgtgg
     6961 gatatcgcat actacagcga aaaacaaaaa cagcacctct acagcatcag cgacgaacaa
     7021 ctgcgtccgt acttcccgga aaacaaagcg gttaacggct tgtttgaagt ggtaaaacgt
     7081 atttacggca tcaccgctaa agagcgtaaa gatgttgatg tctggcaccc ggatgtacgt
     7141 ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca gtttctacct cgacctgtat
     7201 gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact gcgtaggcca gatgcgtaaa
     7261 gccgatggtt cgctgcaaaa accggtcgcg tatctgacct gtaacttcaa ccgcccggtg
     7321 aatggtaaac cggcgctgtt cactcacgac gaagtgatca ctctgttcca cgagttcggt
     7381 cacggcctgc accatatgct gacccgcatc gaaaccgccg gtgtttccgg tatcagcggt
     7441 gtgccgtggg atgcggtcga actgccgagt cagtttatgg aaaactggtg ctgggagccg
     7501 gaggcgctgg cgtttatctc tggtcactac gaaaccggcg aaccgctgcc gaaagagttg
     7561 ctggataaaa tgctggcggc gaagaactac caggcggcgc tgtttattct gcgccagctg
     7621 gagttcggcc tgttcgattt ccgcctccat gccgagttcc gcccggatca gggagcgaaa
     7681 atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg tagtaccgtc tccatcctgg
     7741 ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg gttatgccgc aggttactac
     7801 agctacctgt gggctgacgt actggcggca gatgctttct cgcgctttga ggaagagggc
     7861 attttcaacc gtgaaaccgg gcagtcgttc ctcgacaaca ttctgagccg tggcggttca
     7921 gaagagccga tggatctgtt caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg
     7981 ctggagcatt acggcattaa gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac
     8041 aggcaccgga gacggtgcct tatctgttct ggcggcccgc tgggggctgg agcacgatga
     8101 agacaacctg atggcgctgg tgttaacgcc ggaacatctg gaattgcgca agcgtgatga
     8161 gccaaaactt ggcggcatct ttgttgattt tgttggcgga gcgatggcgc accgacgcaa
     8221 attcggcggt ggtcgcggtg aggcggtggc gaaagcggtg ggcattaaag gcgattattt
     8281 gccggatgtg gtggacgcta ccgcagggct ggggcgcgat gcctttgtgc tggcttcggt
     8341 cggctgccgc gtgcggatgc tggagcgtaa tccagtcgtt gccgcgctac tcgacgacgg
     8401 cctggcgcgt ggatatgcgg atgcggaaat cggcggctgg ttgcaggagc gtttgcagtt
     8461 aattcacgcc tccagcctga cggcgctgac cgatattacc ccgcgtccgc aggtggtcta
     8521 tctcgacccg atgttcccgc ataagcagaa aagcgcgctg gtgaaaaaag agatgcgcgt
     8581 ctttcagtcg ctggtgggac cggatcttga tgccgatgga ttactggagc ctgcacgctt
     8641 gctggcgacc aaacgcgtgg tggtaaagcg cccggactac gcgccaccgc tagcgaatgt
     8701 cgccacgcca aacgcggttg tgactaaagg gcatcgcttt gatatctatg caggtacgcc
     8761 agtgtaaaag taaagcaggg tagcgataac gctaccctgc tttctgcaag aataattaat
     8821 ggctttccgg cgtcgcaatc atgcgtttca gccacggcac catcaacagc atcactactg
     8881 caacgcccag cgtgaccaga ccaatcttac caaacacgtt ggtatagaca ggcaacgtct
     8941 caagcggatc ggtaatgttg tctggtactg cggtaaatgt tgccacatag ccgcccagca
     9001 agaacgcggc agcctgtgtc aggaaccaca tcccgagaat aaagcccatc aaatgctgcg
     9061 gcaccagagc agcaatcatc gccaggccaa gggcgctaat aaacagttca cctaagctct
     9121 ggaataagta caccagcacg ataaaccatg gcgatgtcag cccttgtgca tccgcaaacc
     9181 acattcccgc agctgccgcc gtcaaaaagc ccagtgagca cataaacatg ccgagagtaa
     9241 atttcatcgg catcgagagg tctttgcctt tgttacccag atgcgtgtaa atgcctgcca
     9301 gtattgggct ggcgagtacc acccagaacg ggttaagcgc ctggaagctg accgggttga
     9361 tggaaaaacc gagaatttca tgatgcacgt tgttgatggc aaagaagttc agcgatgttg
     9421 gcatctgggc gtagagaatg taaaacacca ccgcttcgag catcaggaca aaggcgacaa
     9481 acattttatt gcgcccggtt ttatccagct tgaatgcctg acgaaagaag atgatggtga
     9541 cgacgatgga gagaacaatc agcaccagat tggcgacttc tacgttgtgc atcagccagg
     9601 cgcagacgaa gatcatcacc acgctgccaa gtaatacgta caacagtttg ctgaagctca
     9661 ttggccggaa gtcgggctca gaaccaatgt ctttcaccat tccacgacag gcgatgtaaa
     9721 ccagtaatgc gataattaac cccgcaccgc acaggttgta ggtgactgaa tagccgaatt
     9781 tatcagcgat cacaggggcc agcgataacg ctatcaacga gccgatattg atcgacatat
     9841 agaacagggt gaatgtgcca tcaaggcgcg gatctttcgg cggatagcac ttcgaaagca
     9901 agctggctgg gttagcttta aacaggccgt taccgacagc gatagtcccc agggcgatga
     9961 aaatcaggtc aggcttaagt agcgacatgc cggtcatgaa gtagccaatc gccagcacaa
    10021 gtgctccaag aacgatggtg cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa
    10081 tggaaatgag gccatagacc agtgcagcaa aagcaccaaa agtgacaaaa gcctgctctt
    10141 gcgagaatcc aagctgttta acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc
    10201 cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca
    10261 tccccatggg tgttgttgta ttcatattta ttaaccttcc aataccatcc tgaaaagtat
    10321 gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt cagagattct taatattgat
    10381 gcggtgaata gattaataca ctattgcaac agaaaataac cagctgatca cactaatgat
    10441 ggaaaatatg atggtgttaa atattgtatt ggctggggat tttgattgcg aacgcagacc
    10501 gtaggccaga taaggcgttt acgctgatca ggtaacaagg cgcgacaaaa agcaaaacgc
    10561 ccgcatatgc gggcgttcag gaacacgtcg tggagggaag attattcttc ttcgtcgcgc
    10621 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
    10681 agtttgctcc agaagtcctg gtggtgacca caaaccacta gatccatatc gtatttcttg
    10741 attgcatcga ccagaacctg gcccaggtcg ccgctgccgc tcagggtttc agtgattggg
    10801 tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
    10861 tgcatatcac caagattcac atcaataagc ccggtatata ggtcagagta gtttacatct
    10921 acgtggatca gagaaacttt cgcattgtat gggcgagcca tagagactgc tttctctacc
    10981 agaactttgc tttccgggga gagatcgacc gcgatgagaa tgtgtttata agccatagtg
    11041 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
    11101 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
    11161 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
    11221 taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa aaaaacaacc
    11281 tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa gcggtttctc
    11341 caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg ggtggaactc
    11401 cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt tgggctttat
    11461 gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg ttgttagtgg
    11521 tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc ttttttacct
    11581 cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc caacgttatc
    11641 gcgatcatca cgatgatgag tttattcgcc gctgtgagcg ggtgcgtcgg cagtttattc
    11701 tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat tgccttgatg atttggcatt
    11761 aagcacttca gcaataataa cgcataaaaa aagcgggcca gtaaactgac ccgctcgttt
    11821 tggtgcgtac tattacagga actgcaagga gagccagtaa agcccgccgg aaagcagtac
    11881 cgcagccgga agggtaaaca cccaggccat cagaatgctg gtcacggttt tacgctgtaa
    11941 gccgccacca tctaccacca tcgtccccgc gacagaagag gagagtacgt gagtagtgga
    12001 aaccggcatc ccggtataac tcgccaggcc gatagacact gccgcagtca tctgggcaga
    12061 catcccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag tcgttgccac
    12121 acgacgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca tgatgatcca
    12181 caccggtgca tactcgatgg tgctaagcat gtcggacttc agtttcttca acaggcgctg
    12241 atcgtcagca ctcacgccag gcatcttcac cactttgtcg atagtgtcag aaacgcacag
    12301 cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc tttccacatc
    12361 ggtggtcaac atacctttca ggcggttgag cgcgttaatg gtattcgacg gatggcagtg
    12421 gaactccgca ggttgcgttg cgccagcttc cggagccggt actaactgat cagcaccagt
    12481 agcttgtttc agcagcgcag gatgctgctc aaagtaagct tcgacgttgt tgatggcatc
    12541 acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc ctgctggtgc
    12601 gacaccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt ttgcgccgtg
    12661 cgaaaacgcc acgccgatag cggaaaggat cagcgcgata cgcgtccaga acggcggctt
    12721 tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg cgcgtttctt
    12781 ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca ccaggccgac
    12841 aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat tgagtgcatc
    12901 caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga tcgcgccaat
    12961 cagcgtatga gagctggatg caggcaaacc aaagtaccag gtacccaggt tccagataat
    13021 cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca tattaagcag
    13081 cagatccgtc ggcagcatat gcacaatggc ataggcaaca ctcagaccac ccagcaaaac
    13141 acccaaaaag ttgaacaccg ctgccataac cacggcgagc tgagaacgca tcgcgcgggt
    13201 atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg cttcgtagaa
    13261 cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca ggccagcaaa
    13321 caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca ttatcagtga
    13381 ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc tgtatggatt
    13441 tcactcaaaa aataattatc ttatataatt caggcaaata cttcctttta gtaatattga
    13501 tgctggtgcg accactgagg aatctttaca attcacgccc gttttttcta agaggagcgc
    13561 aacgtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat gttctgttct
    13621 gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa aaaaccaggg
    13681 cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaaccg
    13741 ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg ttttacccag
    13801 tgggatttca ttgatctggt caataaacac ggcatcgcct ggcacgagaa aacgttaggg
    13861 caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga tgagtgcgag
    13921 aagggcaatg tgaccgtcag attgcgtagc gaagtgctga gtgtggcgaa ggatgataca
    13981 ggcttcacgc ttgaactgaa cggcatgact gtcggttgtg aaaagctggt catcgcgacc
    14041 ggcgggctgt caatgccggg cctgggcgcg tcgccgtttg gttataagat tgccgaacaa
    14101 tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct gcataaaccg
    14161 ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat taccgctgaa
    14221 aacggcaccg ttttccgtga gaacttactc ttcacccatc gcggcttgtc tggaccggcg
    14281 gtgttgcaaa tttccagcta ctggcaaccg ggggaatttg tcagtatcaa tctgctaccg
    14341 gatgtggacc tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa tcagagcctg
    14401 aaaaacacac tggcggttca tctaccgaag cggttggttg aacgcttaca gcaactcggg
    14461 caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc actgattagc
    14521 acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg
    14581 acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc gcgcaaagtg
    14641 cctgggctgt actttatcgg cgaagtgatg gacgttaccg gctggctggg gggctataac
    14701 ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc agcgaagtcg
    14761 tgctgagcta tttttcctaa agatgaaaat tattagagtc gcattaaaaa tgagctaatt
    14821 ttgatagtgg ctatcttgtg attattttct aatgagcccg tgaactgaac cccccatgct
    14881 taatataagg tggagggaaa ggtgattgaa aactcaccca gtgggctgat ctttataaaa
    14941 tatgaacagg ttgaaaaaaa aacagtaact ttgctgtttt ttatacttaa ccactatgca
    15001 ttaatgctgc gttatttcat tctacctaag aaaaaccagc gttacgcaaa tggttaacgc
    15061 tggttttatc cggtacgttg caattatttt ttagcagaac ccgcttctaa tattgaaacg
    15121 attgagaaca acgtaaagca taccgctccc aaaccgacca gaacccgcga aggaataaaa
    15181 tatcccatat cggtctgcgc catttccgca agaaacgatg ctaggaaaag gcaaaacagg
    15241 caggtgaaga cgggaatcat cggtattctg ttggctaacg aacaggtacg tctccagacc
    15301 agtgccagta gccagacttt tgagaatatg ctgtaacaga tcattcccaa gcaaatgagg
    15361 ataataccgg gagccagtcg ggcgcttgca tcagaactga ctaagacgta tatgccctgc
    15421 agcaccgtga ttgagccgag aaaaataacc caataacacc acagccagtg ctctttcgtt
    15481 gaaaacgtat tacgtgtttg atggacgatt gtggcaacaa ggccaattag acaggcacag
    15541 attgcggtta acccgagcaa tacgtgaccg gcgacataat gcggagttat gtcggcacta
    15601 cgtaacagcg taatagacca aatgaaaccg agcagggtga gtaacacggg aacggcaatg
    15661 aggcaattac cgattaatga agaataagcc tgtaccggtg ttccgtcgct cttgctcccc
    15721 gctgcgtttt taggaatgag cagaaagtga ccggatgacg ctgccaccgt cgatacacag
    15781 gcggcaatca taccaacgcc gaaaataaca tggccggcga caaactcgtc tgccatcaca
    15841 tcattacctg ctaacagtgc ccaaccccat atcatggtga taattgaccc cgcatagcca
    15901 ataatgggga acaatgtatt gtaaaacgta ttaacgccgc gcgtgagctg cgaaatgata
    15961 ataaatgcgg tagtgaataa tgccaggcat atggctgcca gagaaatgag cacatgaccc
    16021 gcgacgaaat actcgctttg ccctgaactt tccagcacaa agccacctaa cgcgatacaa
    16081 atcaagccca taattaaagg gattaatttg aaaagccacc cgatatatat gttcacttta
    16141 aactcctcag gtatccctgc taatattgaa atgtgcatta atgataattt cagcctgtta
    16201 atacaatgac gagatatcat taaagacaaa tgcatagcat aactatagcg gggtttactt
    16261 tgcagagcgc tacttttcag cgttcaaaag gccatgataa ataaatattt atcctaagat
    16321 taattcttat
//
LOCUS       DAZFTN010000104        15905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFTN010000104 DAZFTN010000000
VERSION     DAZFTN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            137..646
                     /gene="ybgA"
                     /locus_tag="ACHKV2_003782"
     CDS             137..646
                     /gene="ybgA"
                     /locus_tag="ACHKV2_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO6941589.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHKV2_003783"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHKV2_003783"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO6941590.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHKV2_003784"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHKV2_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO6941591.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHKV2_003785"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHKV2_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO6941592.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHKV2_003786"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHKV2_003786"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO6941593.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHKV2_003787"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHKV2_003787"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO6941594.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHKV2_003788"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHKV2_003788"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO6941595.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHKV2_003789"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHKV2_003789"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO6941596.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /gene="abrB"
                     /locus_tag="ACHKV2_003790"
                     /pseudo
     CDS             complement(7859..8905)
                     /gene="abrB"
                     /locus_tag="ACHKV2_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
     gene            complement(9054..10115)
                     /gene="ybgO"
                     /locus_tag="ACHKV2_003791"
     CDS             complement(9054..10115)
                     /gene="ybgO"
                     /locus_tag="ACHKV2_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6941597.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHKV2_003792"
     CDS             complement(10112..10843)
                     /locus_tag="ACHKV2_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6941598.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHKV2_003793"
     CDS             complement(10858..13317)
                     /locus_tag="ACHKV2_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6941599.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13367..13933)
                     /locus_tag="ACHKV2_003794"
     CDS             complement(13367..13933)
                     /locus_tag="ACHKV2_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6941600.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHKV2_003795"
     CDS             complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHKV2_003795"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO6941601.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15809..>15905)
                     /locus_tag="ACHKV2_003796"
     CDS             complement(15809..>15905)
                     /locus_tag="ACHKV2_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941602.1"
                     /translation="YEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT         3909 a         3880 c         4267 g         3849 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctggttgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg gtttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgctagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccatcgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gcgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtccagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tccttcatgg gctatgataa agtatactaa ttattccgac
    13981 agaatagtgg ttttttgttc atgtaataat attaatgttg ctacattatg tttgagagtt
    14041 tctcaaagct ctcgaatcat aaataaagtg aattaatcct attacgagtt tatttaattg
    14101 tgggtgctac ccaatgtatt ctacatcgat tggcgggata acggtggtgg tgaatgaaaa
    14161 attaggattt tatttagtta tcggatatgt attcttgtaa cgtgtaatta tgtatttgtg
    14221 tccgattacg tttttttgtc gatagacata actaacgaac aaaaaaaagc ccgccagata
    14281 ttcaggcggg ctaaacgata tatggttcaa atcagataat taatgtttaa catctgattt
    14341 aaagtctcgt ttttcatagc cggtgtacaa ctggcgcgga cgggcgatct tcatgccgtc
    14401 actgtgcatt tcgctccagt gagcgatcca gccaacggta cgggccatag cgaagatgac
    14461 ggtgaacatg gaagacggaa tacccatcgc tttcaggatg ataccagagt agaaatcgac
    14521 gttcgggtac agtttcttct cgataaagta cgggtcgttc agcgcgatgt tttccagctc
    14581 catagccact tccagcaggt catctttggt gcccagctct ttcagaactt catggcaggt
    14641 ttcacgcatt acggtggcgc gcgggtcgta atttttgtac acgcggtgac cgaagcccat
    14701 caggcggaaa gaatcatttt tatctttcgc acgacgaaca aattccggaa tgtgtttaac
    14761 ggagctaatt tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt gtgcaggccc
    14821 ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg aagagccagc
    14881 ggtacgcacg gtggaggtag aggcgttctg ttcatggtca gcgtgcagga tcagaatacg
    14941 gtccatagca cgttccagaa tcggattaac ttcatacggt tcgcacggcg tggagaacat
    15001 catattcagg aagttaccgg cataggagag atcgttgcgc ggataaacaa atggctgacc
    15061 aatggaatac ttgtaacaca tcgctgccat ggtcggcatt ttcgacagca ggcggaacgc
    15121 ggcaatttca cgatgacgag gattgttaac atccagcgag tcgtgataga acgccgccag
    15181 cgcgccggta ataccacaca tgactgccat tggatgtgag tcgcgacgga aagcgtggaa
    15241 cagacgggta atctgctcgt ggatcatggt atgacgggtc accgtagttt taaattcgtc
    15301 atactgttcc tgagtcggtt tttcaccatt cagcaggatg taacaaactt ccaggtagtt
    15361 agaatcggtc gccagctgat cgatcgggaa accgcggtgc agcaaaatac cttcatcacc
    15421 atcaataaaa gtaattttag attcgcagga tgcggttgaa gtgaagccag ggtcaaaggt
    15481 gaacacacct tttgaaccga gagtacggat atcaataaca tcttgaccca gcgtgccttt
    15541 cagcacatcc agttcaacag ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc
    15601 catttaaggt ctccttagcg ccttattgcg taagactgcc ggaacttaaa tttgccttcg
    15661 cacatcaacc tggctttacc cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa
    15721 acctgggtac agagctctgg gcgcttgcag gtaaaggatc cattgatgac gaataaatgg
    15781 cgaatcaagt acttagcaat ccgaattatt aaacttgtct accactaata actgtcccga
    15841 atgaattggt caatacttcc acactgttac ataagttaat cttaggtgaa ataccgactt
    15901 cataa
//
LOCUS       DAZFTN010000105        15857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFTN010000105 DAZFTN010000000
VERSION     DAZFTN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(258..851)
                     /gene="tehB"
                     /locus_tag="ACHKV2_003797"
     CDS             complement(258..851)
                     /gene="tehB"
                     /locus_tag="ACHKV2_003797"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO6941603.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(848..1840)
                     /gene="tehA"
                     /locus_tag="ACHKV2_003798"
     CDS             complement(848..1840)
                     /gene="tehA"
                     /locus_tag="ACHKV2_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO6941604.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            1964..2944
                     /gene="ydcK"
                     /locus_tag="ACHKV2_003799"
     CDS             1964..2944
                     /gene="ydcK"
                     /locus_tag="ACHKV2_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO6941605.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2939..3475)
                     /gene="rimL"
                     /locus_tag="ACHKV2_003800"
                     /pseudo
     CDS             complement(2939..3475)
                     /gene="rimL"
                     /locus_tag="ACHKV2_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(3538..3762)
                     /gene="ydcH"
                     /locus_tag="ACHKV2_003801"
     CDS             complement(3538..3762)
                     /gene="ydcH"
                     /locus_tag="ACHKV2_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO6941606.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(3902..5557)
                     /gene="opgD"
                     /locus_tag="ACHKV2_003802"
                     /gene_synonym="mdoD"
     CDS             complement(3902..5557)
                     /gene="opgD"
                     /locus_tag="ACHKV2_003802"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO6941607.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5782..7125)
                     /gene="ydcJ"
                     /locus_tag="ACHKV2_003803"
     CDS             complement(5782..7125)
                     /gene="ydcJ"
                     /locus_tag="ACHKV2_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6941608.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7342..8265
                     /gene="ydcI"
                     /locus_tag="ACHKV2_003804"
     CDS             7342..8265
                     /gene="ydcI"
                     /locus_tag="ACHKV2_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6941609.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8303..9943)
                     /gene="trg"
                     /locus_tag="ACHKV2_003805"
     CDS             complement(8303..9943)
                     /gene="trg"
                     /locus_tag="ACHKV2_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO6941610.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            10342..10491
                     /gene="hokB"
                     /locus_tag="ACHKV2_003806"
     CDS             10342..10491
                     /gene="hokB"
                     /locus_tag="ACHKV2_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO6941611.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(10469..10537)
                     /gene="yncP"
                     /locus_tag="ACHKV2_003807"
     CDS             complement(10469..10537)
                     /gene="yncP"
                     /locus_tag="ACHKV2_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO6941612.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10563..10736)
                     /locus_tag="ACHKV2_003808"
     CDS             complement(10563..10736)
                     /locus_tag="ACHKV2_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941613.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(10981..11511)
                     /gene="cybB"
                     /locus_tag="ACHKV2_003809"
     CDS             complement(10981..11511)
                     /gene="cybB"
                     /locus_tag="ACHKV2_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO6941614.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11700..12701
                     /gene="gap"
                     /locus_tag="ACHKV2_003810"
     CDS             11700..12701
                     /gene="gap"
                     /locus_tag="ACHKV2_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6941615.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12743..14182)
                     /gene="aldA"
                     /locus_tag="ACHKV2_003811"
     CDS             complement(12743..14182)
                     /gene="aldA"
                     /locus_tag="ACHKV2_003811"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6941616.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14379..15179)
                     /gene="ydcF"
                     /locus_tag="ACHKV2_003812"
     CDS             complement(14379..15179)
                     /gene="ydcF"
                     /locus_tag="ACHKV2_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO6941617.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15295..15672)
                     /locus_tag="ACHKV2_003813"
     CDS             complement(15295..15672)
                     /locus_tag="ACHKV2_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941618.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
BASE COUNT         4135 a         3985 c         3665 g         4072 t
ORIGIN      
        1 aagcgccgct tcgtgttgtg gcaaaaaaag tgttggtgaa aataaatttt tttagcccct
       61 tttcagacga catagtcaat gccgcaaaag gggctaagtc aatcatcagt agttaaattt
      121 cagtaagtta agctgctaaa aaacttcgac tactgtgatt aatttcacat cccttcgata
      181 aaaaatatgc ttaccgaaaa gaaggacagt aaaccatctc agcgtgggga attttcacgc
      241 gctttgttct taccgggtca ttttttacgt gccagcatcg tggcgaaacg cagtttaata
      301 cgattaccgt tggcgtcggt gcggtgcagc tcgccgacgt cttcattgta tttcaccatc
      361 tcccagcctt cgtaatatcg acgtaattct ccctctttga aggcaaacgg gaagccgacg
      421 gtacatggat aatcagcggt atccatcgcc gccacaatca ggttgtaacc gccaggttta
      481 gtgcaacgtt gcatattggc aatcagccct gggatggttt tagcctcaag gaacatcagc
      541 accacagtcg aaagaataaa atcgtactct ccatcaaatg tgaggttatt cagatcaacg
      601 acccgggtgt gtaaattatc cagattttca atggatttaa tgcgctcgac gttggcgata
      661 ctcatggcat ttttatccca tgcgtcaaca tcataaccat tggctgccag gtaaagactg
      721 ttacgaccat tgccacagcc cagatccagc gttttacccg gtttaaccac tttcaccgct
      781 tccagtactt cagagtgtgt gcgggttaat tcatatttat cagtaaaata attttcgtca
      841 cgaatgatca ttctttgtcc tctgctttca ttaaaacggc gcgctcggtt ctgactaaca
      901 attttccctg catcagaagc gccaaagtac ggatgagcag tattgcaata ataaaattgg
      961 taaagataaa cagcggcacc gccagcgtat ggaaaaatcc attatcgctg ccactcccca
     1021 gatgcaaacc ggtggttgcc agtgcagata cgccgaacga gaaactccag aatgaagcat
     1081 taaatggctg ggagagatac catggcatca gacgtagcat aaacagcagt tgcagcagtc
     1141 cataaccgaa aagcattttc gccagcgtgt caccctcgcc gccgttgacg ctcagccagg
     1201 cactacaggc caccagcgca ggagcgagct gaatgccgag tgatgtccgc agtgccgtgg
     1261 gtaattctcc cgaactacgc agacgctgca agatcaccgg ttcaaggctt agccatgaga
     1321 aaacgcctgc gcctaaaaac accagaccgg cgtcggtgta gcccaacgca ccacaggcca
     1381 ttgcgctgat aaagttgttg gcaactgtcg gcagatacag tccaggcgta gtagcttctt
     1441 cagggtgaga tccgcgccat aatcccgcag tttgccaggc ggcataagcc aactgaacca
     1501 cgacaccaaa actgaacagg cacaccgcca gtgggcgaaa ccacggaaca aaaccaatcg
     1561 ccaccagcat cgtcgttgca ggaaacaaac tcacaaaact gctcagcact ggatggcgaa
     1621 cttccgccag cacgctatgc ggaaagcgta tgagtcgggt aataaatgcg ctagtcaata
     1681 atccccagat gatcatcgcc agaatcacca gcccatcccc taaccagtgg ctgacctgcc
     1741 aaacctggct ggcatagcgc caggcaaatc ccatccctat cgtccccaac acaataccaa
     1801 agtagcctgc cggcaaattg agcactttat cgctctgcat cttcttactc attttgttta
     1861 atttataaag tatatttgaa atgcatttta tggaattttc tcgagcatag ccagagccgc
     1921 agaatttgct acggttatag taagtcacct gtgggaaaac gctatgcgca aatatcgtct
     1981 aagtgaagaa cagcgagcct ttagttatca ggaagatggc actaaaaaaa atgtgttatt
     2041 acggcagatt atcgccatta gcgattttaa cgatgtgata gcaggaaccg ctggcggctg
     2101 gatcgatcgc gaaacggtgt tagcacagga aggaaattgc tggatttacg accagaatgc
     2161 tattgcgttc ggcggaacgg tgatttccgg taatacgcgc atcaccggca ccagcgtact
     2221 gtggggagag gtttacgcaa cggataatgt ctggatcgat aatagtgaaa tcaaccaggg
     2281 tgcatatata agtgacagtg tcaccattca tgactcactg gtttgcgggc aatgtagaat
     2341 atttggtcac gccttaattg atcaacattc tatgatcgtc gccgcacaag gcttaacgcc
     2401 cgaccatcaa ctcctgctcc aaatttatga tcgcgcaagg gttagcgcct cgcgtattgt
     2461 ccaccaggca caaatttatg gcgatgccgt catccggtat gcttttattg aacatcgcgc
     2521 cgaagttttt gattttgcca gtattgaagg taatgaagag aacaacgtct ggctatgcga
     2581 ttgcgctaaa gtctacggtc acgcgcaggt gaaagctggc atagaagaag atgccattcc
     2641 aacgattcat tacagttcgc aggtggcgga atatgccatt gtggaaggta attgtgtgtt
     2701 aaaacatcat gtcttgattg gcggaaatgc cgtggtacgt ggcggaccga ttctgctcga
     2761 cgagcacgtt gtaatacaag gtgagagccg tattacaggt gcggtgataa ttgaaaacca
     2821 tgtggagtta accgaccacg ccgtcgttga agcctttgat ggcgacacgg ttcatgtccg
     2881 tggtccaaaa gtcattaacg gcgaggagcg cattacgcga acgccgctgg cagggttatt
     2941 atgaatcgat aatacgcgcg tataagttca catcatcata ggcatcattc aggaactcag
     3001 cctgtttcag gcaaccttca aggataaaac cattgcgcaa agcgacctgg ttgctttgcg
     3061 gattgtccac ccgacatttg atcacgaagc gtctaagttc accagactga gcgtaatgat
     3121 gaatcagtgc ctgcagcgcc tgagaaatga tcccctgccc ctgatgagat tcgtccagtc
     3181 agtagccgat ttcagcggtt ttattcagtg gttcaatacg attaaacgag ataacgccga
     3241 taagttcatc ttctttgaaa atcatgaaca ttttggcata gccgcgttga tgcaacatca
     3301 cattaccctg caccgttttt cgcgtgtcct cttcaatctg aacgaactgc ggccagttta
     3361 gcgactgctg taaccaggtt ttatttttac agattaactg ataaagagga gtgacgtgat
     3421 tttctgcaac agcatgtaat tcaagtgatt cgcttacttt tatcgtttca gtcatcttat
     3481 ctccagtcaa tccatgcagt tgttaaaaaa gccgcctggc tggcggctta ggaaagttta
     3541 cacctctttg acgctctcat gctgcaggat tttgagcatc tcatctttta actgtagctt
     3601 ttgttttttc atgcggacca cttccgcatt gtaccctcga tcgtcggaac cttcctttct
     3661 ggcaatttca tgatcaagtt tattgtgttt atcgaacaag gacataaagc gaggattttc
     3721 gtttttcaga cgggatatta aatctcgata ttctggaaac atacgcactc cctgttagtg
     3781 gctgacaagg tgtatctgaa atacacacca tcagattact catttaactt agggaaaaat
     3841 gcgagcggga tcgtgtttaa ccagacggca cctcccgaag aaggtgccga agaaaaaacg
     3901 attaactcat cacgcggtcg tcaacatact gacgtttatc cggcgctggc gggaaatact
     3961 gatacagcca tgtttcactg atagcgtcgc cctgacaacg cagatacatc cgcatatcga
     4021 ccggatcagt ggagtccgaa gtcggatacc agtcaaactg aatacgataa ccatcaatgg
     4081 gttcaatata gagaatttcg atttgcttcg cttccccact ggaaagcgta atcaccggct
     4141 caatgccttt tggtgcggca gctttcagat caccaccaac gaaatcgacg gcaaaacgac
     4201 gcgcccattt ttcgggatag tgttcacctg gtgcccaacc ttccgggaaa ccgcccatgc
     4261 cggtacgcgt cgccataacg cgcgctaatg ggcaatgaac aggcggttgc gcactccagt
     4321 acagacgata ctggaatgca aactcatcac ccgcctttac agctttttct ggctgccaga
     4381 agcagacaat gttatccagc gtttcgcccg ttgttgggat ttccatcagg ccgatggtgc
     4441 ccttacccca cttgttacgc ggttccaccc acagacttgg gcgtttgtta taccagccca
     4501 taatgtcctg ataatgggag aaatcacgat ccagttgcaa taaaccaaac cctttcgggt
     4561 tgttgtcggt gtaagcattg aactgcaatt tttgcggatt attcagcgga cggcaaatcc
     4621 actcgccgtt gccccgccac atggacagac gatcagagtc atgaatttgc ggatgaatgg
     4681 tgtcgcacat ccgacgttca ttagtaccgc agctgaacat actggtcatc ggcgcaatgc
     4741 ccagctgttt aatgtctttg cgcgcataca ggtgattttc cacatccata atcacctgac
     4801 ttttctcaca atggatagtg aacttatagg caccagtaat gctggcgcta tcgagcaacg
     4861 cataaacggt aaaggtagtt gcccccggtt ttaccgtatc aaaccagaag gcggtaaagt
     4921 cggggaactc ttctttactg tcggtgtaag tgtcaatcgc caggccgcgg gccgacaaac
     4981 cgtattgata tgtgtcatca acggcgcgga aataactcgc tccgaggaat gatactacat
     5041 cacggcgcgc cagttcgggg gctttaaaca cgcgaaaacc ggcaaagccg agatcgcttt
     5101 gcccttctaa ttgtttggta tcaacacctg catcgttgta tttgaacaac tccgggcgaa
     5161 agtgaatttc acgcgccaga tgtgttgctg gatctacaga aaacatacga acgcggcgac
     5221 ggaatcccat tcccatatgg aagaactgag cgtccagttg acggttctca acgttatgcc
     5281 agagcgattt ttcggcgtcg tattgaatac tgttataagc ctgcggcgtc attgtcgcca
     5341 gcgtgtcagg taacggacga ggcgcaccac gccacgctgt ttgcgctaag tcgtgcgcca
     5401 ttgactgtag aatggagaag tcaaaacgct gggtttgccc gtcggcaata tcagaatctg
     5461 ccgcgaatgc cgcctgagaa aaaagagaag caatgccgct ggtaccgcac acggcggcca
     5521 tagccattga acctttaata aatcgtctac gatccatacc tgaaagtgag tccttctggt
     5581 gttgtgaatg gtcccgcgct ggtcgttttc agcggcaaga aagtgcttat ttatgaccgc
     5641 tcactctaga caatatttat taataatcca attgttgaac tgagagaata tgcataaatc
     5701 ggcaattttt ttatgtcgat gggatgagcc tgattcgttt gtaaaatcag gcaaattttt
     5761 gcaaaggcgg atggtcagaa tttaaagcaa accacagcga cgtttactgc gttcttccgc
     5821 ttcctgataa agctggaact catccaaaac cggacaaccc aacgcctgct caaatgcttc
     5881 gcgactggca ttaccgtggc tgcgtgcctg cgtttcatta cccagatttg actggaagat
     5941 ccccgccgcg ctaacgggca agaaatcttc ataggtaatg ggttgcgccg cgacccaacc
     6001 acgttcaatt aagggctgtg gatcgtctcc gggatgaatc gcctgacgat gcgcctcacc
     6061 tgaaggcgtc agacggtacc ggaaccatgc cagcccttgc tgacgcatta aaaactcact
     6121 gtcaggaaaa gtgcggaagg tttcctgtaa atgcatttgg tgagtgagat tatcctgccc
     6181 ggttccagcg ttacgcagaa gatcatcata cagttgtcga cctttcggcg ttaatgccac
     6241 gccacgctgc tcaatttcac caaagcgcgc agtatgcgtg ccctgtttct gccccgcaaa
     6301 caacaccgtc tcttccagtg ctttaaagct ggtctggcgt agtaaaatcg gtacctcgcg
     6361 gcgcggcggc ccttcgatca gaatttttgg ttcaattccg cattcaggca tcatcgactg
     6421 cacccggtca atatccagcg tacgtggcgt cagatggttg atatggcatc caggaaaaca
     6481 gaccacatca gcaatcaacc gatgttcgtt gtgcaaggcg cgataggttt cttcatctac
     6541 cgttgctgac tggtgccaac ggaacgtttc cagggcctcc tgcacaaact cctgtgcctg
     6601 tgtttcgtta aaaccgcccc gctggtcata ctcctctaac agttgtcgac aacgtggggt
     6661 gaagatatcg cgctgacgca gaatctccgc cgctttctgg cgcaaaattt cgttctcgat
     6721 aagctcaagg cggagtaagg aggtaaaaac gcggaaggga ttacgcgcca gagaagcatc
     6781 atcaatgggc cgaaatgctg tcgaatgtac cggcacccct gcctgcgaga gatcgtaata
     6841 gctcaccggg tacatcccca taatggcaaa catccgccga agagtagcaa gctcttgtgc
     6901 agtcccaacg cgaatcgccc catgacgttc aacattcagt cgcgccagct cgtctgcatt
     6961 taccattttt tcgtgcagtt gaggattgtt ttccagcaca gccagattca catcagctac
     7021 cagttccagc agcgtgccat attgcggaac ttcttgctgg tacatggctg acattgcctg
     7081 cgaaaactgt tcccgaatct catccgccgt gatgctgttc gccatagtgt ctgcctccag
     7141 tggtcagtaa tctggagtgt aggtaaccgc attcactctg gcgggaagaa tttacaaact
     7201 gtgatctcgc cgcgaaaaca tcaatattat ccattttgct gtaacataat tgctttaatt
     7261 gttaataata ttttgcaatc aagttatcat aatcaaacaa cttcacttgt cagcgacacc
     7321 gcttcgtttt taacatcgct tatggaaaaa aatagtctgt ttagtcagcg catccgtttg
     7381 cgccaccttc atacattcgt agctgtcgca caacaaggaa ctttggggcg cgcggctgaa
     7441 acccttaatt tgagtcaacc tgcgctctct aagacattaa atgaactgga gcagctgacg
     7501 ggcgctcgct tgtttgagcg tggtcgtcag ggggcgcaac ttaccttacc cggcgaacaa
     7561 tttttaacgc atgcagtcag agtacttgac gccatcaaca ctgccggaca gtcgcttcat
     7621 cgtaaagaag gtcctaataa tgatgtcgtc agggttggtg cactacctac tgcggcactg
     7681 gggatattac cttcggttat aggtcagttt catcagcaac aaaaagagac gaccttgcaa
     7741 gttgcgacaa tgagtaaccc tatgattctg gcgggtttga aaaccgggga aatcgatatc
     7801 ggcattggtc ggatgtcaga tcctgaactg atgaccgggc ttaattacga actgctgttt
     7861 cttgaatcgc tgaagctggt tgtccgccct aatcatccgc tacttcagga gaacgtaacg
     7921 ctaagccggg tgctggaatg gccggtcgtt gtatcaccag aaggcactgc gccacgccag
     7981 cattcagatg cattagtaca gagccaggga tgtaaaattc cttcgggttg tatcgaaacg
     8041 ctgtctgctt cgctatctcg tcaacttacg gttgaatacg actacgtgtg gtttgtccct
     8101 tctggcgcgg taaaagacga cctgcgtcat gccacgctgg tggccctgcc tgttccggga
     8161 catggtgcag gcgaaccgat tggaatactg acccgcgtag atgcgacgtt ctcttctggt
     8221 tgccagttga tgattaacgc tattcgaaaa tcaatgccgt tctgaaaggt gaagggatct
     8281 gtcgatccct cctttaacat tttcacaccg gagcgaaact aactggttca cccgctccgc
     8341 gaggttctgc caacacagat tgtttgttca gacggaatac gtccaccgcc tcagttaatc
     8401 gtgccgcctg ttcttcaagg gacaccgccg ccgctgaggc ctcttctacc agagaagcat
     8461 tctgttgcgt caccttatcc atttcagaaa tcgcctggct aacctgcgtt atgcccctac
     8521 tttgctcatc cgaggcggcg gcaatttcct gcatgatatg tgtgacactc gcgacggcat
     8581 caacaatagt gctcatggtt ttcccggccg ttgccacctc atccgatccc aggtcaatta
     8641 acctgactga ttcactgatc aagccttcaa tttcttttgc cgcctgggcg ctgcggcttg
     8701 cgagtgtccg tacttcgctg gcgacaacgg caaatccacg cccttgctca cccgctcgcg
     8761 cagcttcaac ggcagcattc agtgccagaa tattcgtctg gaaagcaata ctgttgatga
     8821 cggcggtgat ctcagaaatt ttcttcgaac tcgtggagat agcgcccatc gtttttacta
     8881 caccggaaac cgtctgcccg ccatcgctgg ctttaataga agcctcttgc gccagtttgc
     8941 tggcataatg cgcgttatcc gcattctgtt tcaccgtcgc agtgagttgc tccatgctgg
     9001 cggcagtttg ttcgatagcc gccgcttgtt cttcggtgcg agatgacagg tccgcattgc
     9061 cagctgaaat ttcgctggtg ccacgataaa tctcttccgc accctgtcga acagtcccta
     9121 cggtcatccc cagtgaatgc tgcatttgct gtaaatgacg acttaagcga ccgatttcat
     9181 tacgacccgc cggttcatcc ttcatcgtca gatcgccact agcgattttt tcaatccgtt
     9241 gtgcggcatg ttgcagtgga cgaatgacga tccgacgtag caccataaat gttatcagcg
     9301 tcatgaccag ggcaagcaca aacgcaccaa tcatgaacat cccgcccagg cgggtgcgct
     9361 gatgggccag ttccgccagt tggttggctc tggtgctacg tatcttaacg gctttgttca
     9421 aaatatcggt ataagcatta tccagcgttc ggatctgctc actttcatga ttgataatcg
     9481 cttcaaacat gccatttttg gcatatttca acataggttg catacccgtg atataagcct
     9541 gaaagcgttg atttaattca gtgtcgaggg cttcatcagc aggtgttttc accggccgat
     9601 tctgataagc acgataacct tgctgcgact gtttaatctc cgattcggct tgcgcaatat
     9661 ttcgcttcat tgcttccatt tccgcaatac gactcgcagc cccggcctga atcatattga
     9721 tccgggctga acgcaaaaaa tctgaactgt tcgctaaccc ggtccgaatc tcaatctccg
     9781 cagtaacatc acgctgatcg cgatcggcct gccagaggaa atagccagcc agggctgaac
     9841 ttaatgcgaa tagaactaag ataccgccta gaatgcaggc aaataacgga accaacctga
     9901 tgtgatgcaa aaaacctaat cgctgtgagg gagttgtatt cattaccgtt gtctctcgtc
     9961 caggtttact cctgatagaa agtcatcggc atttgacggt aattacttac gactaaaagc
    10021 ctctttgcgt cacattttta caacaattat tgcagagaag attaatgcca ttggaatgaa
    10081 gagagtaacg aatggttaaa ctggctaacc cgaatgacga aaaattctgg aaaacagcac
    10141 gcaatttcgt caggtgatta ttttcgtccc taaggaaacc acataggatg ctcacgggtg
    10201 cctgagactt tctgttttca ggctaaccgg gaatcagaaa gacgaaggcc ccgaccgata
    10261 tttctatcaa ccgaggccaa cgaatgaacc aagcaattca gatgttagcc tcttacccgc
    10321 catccggcaa ggagaaaggc tatgaagcgc aaccctctgg tggtgtgtct gctcattatc
    10381 tgcattacga ttctgacatt cacactcctg acccgacaaa cgctctacga actgcggttc
    10441 cgggacagtg ataaggaggt tgctgcgctc atggcctgca cgtccaggta agggcaagcg
    10501 cggggatttt ccccgcgcat ctgactagca gattcatttt ctcatggcac cccttttaca
    10561 cttcagttag tgcatgtttt tttcgatgca ctaatagaac catcattaca gacaaatttg
    10621 ccgcctgccg tacagtgtga gatcccccct ttcgaaccag aacagggttt gcgcccggca
    10681 tcggcataaa aggaaacaag cgctcccata aacaaaataa gtgccagttt tttcatataa
    10741 cctccatggt ttatcgaatc ttatgttcga tttgaacgtt atacgctggt ttattttgat
    10801 accgttgatg agatcaaaga tcgggtgaat tgattaaaaa taagccagat ggacgtatgg
    10861 gcaaggatta tggttttatt tgtcatacaa ataaatataa taggcgcttc cgatgtagac
    10921 ccgtattctt cgcctgtacc acgggtcggt tttagtacag gcgttttctt taaatatcct
    10981 tcaggaacgt ttacgcggca tcatgcgtag aagtgtgttg tccttccaga aatagtggtg
    11041 tgccagtgca gccgcagcgt gcaaaccgat gacaaaatat cccagattcg ccagcgtttc
    11101 atgccacgac tttaagctat ctacccgttc gaaattggcc tctgaagcgt aaggcatcgt
    11161 caaaccaaac gcaaaccacg ggttgcctcg gttatacatc atcaccaaac caatcactgg
    11221 cagcgcaata aacaggagat aaatcaccaa atgtcccaaa tgcgccagtc ccgtcatcat
    11281 cggttttggc ttaggtataa tcggcggggt tgggtatttc agccttaaca aaagacgaac
    11341 gaccatcagc acgagaattg atatgccgca ggaaacatga atcatgttga tgagtggccg
    11401 atcgctacgt gggaagaaac cccgaaactc cattgcgcaa tacgctgcga taaccagcaa
    11461 aaagaccagc cagtgaatgc tgatttgtaa ccttgagtat ttattttcca taacatttcc
    11521 tgctttaaca taattttccg ttaacataac gggcttttct caaaatttca ttaaatattg
    11581 ttcacccttt ttcagtacgt tacgtttcac tgtaagaatg gagattgccc atcccgccat
    11641 cctggtctaa gcctggaaag gatcaatttt catccgaacg ttcctgacag gagaaaacca
    11701 tgagtaaagt tggtattaac ggttttggtc gtatcggtcg actggtgttg cgtcgattac
    11761 ttgaagtcaa aagcaacata gacgttgtcg ctattaatga tcttacttcc ccaaaaattc
    11821 tcgcctatct gctgaaacat gattcaaact atggtccgtt cccctggagc gttgatttta
    11881 cggaagattc acttatcgtt gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta
    11941 aaaatattcc gtggaaagcg aaaggtgcag aaatcattgt cgaatgtact ggcttttata
    12001 cctccgccga gaaatcgcag gcgcatcttg atgctggcgc gaagaaggtg ttgatttccg
    12061 cccctgcagg tgaaatgaaa actatcgttt ataaagtcaa tgacgacact ctggatggca
    12121 acgacaccat tgtttccgtg gcatcatgca ctactaactg tcttgcgccg atggccaaag
    12181 ccttgcatga cagtttcggg atagaagtcg gcacgatgac gaccattcat gcctatactg
    12241 gcacccagtc actggtggat ggcccgcgtg gtaaagattt acgtgcttca cgcgccgcgg
    12301 cagaaaatat cattccccac actacgggcg cggcaaaagc cattggtctg gtgatcccgg
    12361 aactgagcgg caaactgaaa ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca
    12421 ctgaactggt atcgattctc ggaaaaaaag tgactgccga agaggtgaat aacgcactta
    12481 aacaagcaac caccaataac gagtcatttg gttataccga tgaagaaata gtctcttccg
    12541 atatcattgg cagccatttc ggttcggtgt ttgatgccac gcaaacggaa attaccgccg
    12601 tgggcgattt acaactggtg aaaacggtcg cctggtacga caacgaatat ggcttcgtca
    12661 cgcagcttat tcgcaccctc gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa
    12721 aaacctccgc ctctttcact cattaagact gtaaataaac cacctgggtc tgcagatatt
    12781 catgcaggcc atgtttacca tctgcgccgc caataccgga tttacgccat ccggcgtgga
    12841 agccttgcat agcttcgaag ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt
    12901 taatggcttt catcgcgacg ttcagatttt gggtatagat tgatgaggtc aggccgtaat
    12961 cactgtcatt agccattgag atagcatctt ccagcgtgtc aaatgcgact accggcagaa
    13021 ccgggccaaa ggtttcctca tgcataatcg acatttcctg gcgaacatcc agcagcaatg
    13081 tcggcggata ataatatcct ttcccctcta ccgctttgcc acccaacgcc actctcgccc
    13141 cttcttctac tgcgcgcgcc actttttgct cgaccctttc cagcgccgcg gcgttaatca
    13201 acggccccat cgcaatgtcg ttgcgttcag cggggttacc aaattgaacc gcctgcatcg
    13261 cttcacccag ccgattgacg aactgatcat aaatgccttt ctgtacataa acacgttctg
    13321 cacagttaca cacttgccca ctattaatga cgcgtgaatc aacgatggct ttgactgcca
    13381 gttcaagatc ggcatcgtcc attacgatag ctggtgcttt accccccagt tccaggcaca
    13441 ctttggtgat gtttttcgcc gcagtcgcca tgatcttctc acctgcagag acgctgcctg
    13501 tcatactgac cattgcgacc tttgggttac ccgccagttc ttgcccaacg gtttcaccac
    13561 gccccagaac aaggttaaac acgccgcgcg gaaggcctat ttcatcgacg attttggcga
    13621 atgcaatcgc attgtttggc gtaaattcac tgggtttaat gacaatggta ttaccagtca
    13681 aaagagcggg agccattttg cgggcaatga ggaagaacgg gaagttccac ggcagaatgc
    13741 cggtagtcac accaagcgca cgtttaaaca gaagaatatt ttctcctgga cgatcgcttt
    13801 gaataatctc gccctcgtaa cgccgtgccc actccgccat gtaatcgata tagtcggcag
    13861 taaaagccac ttcgacttca gccagctgct ggatcttgcc cccttcttca acaatcagcg
    13921 cactgatttc actggcgcgt tcgcggatcc cggcggagat tttgcgcaac caactggcgc
    13981 gttcaattgc aggcaacgct tcccattctg gttgtgcacg ttctgctgca tcgattgcct
    14041 tacgggcatc ctcggcctga ccatcgggaa tgcgggaaat gacagcctct gtagcagggt
    14101 ttaccacatc aatccatgcg tctccacgcc aggtaacaaa ctgtccatca atatacatag
    14161 gatgttgaac gggtactgac atgggcgact cctgtgattt atatgtttgt ttttggcaaa
    14221 tacattgtta attaacatca atcatcatag agacggcatc atgaaaggac aattctgtga
    14281 agggcttcat aaaaacaagt gtaaaaggaa taagaggcag aaataaaagg caggagttat
    14341 gcccgtttgc aaacaagtgg ttcacaaacg ggcaattttt aacgtaacga gcgactttcc
    14401 atcgcctcga tgagcaccgc atcgtgtttc agcgtttgcc atgcatggat gacttctgcc
    14461 ggaaaatcaa cgtgaacgat aaaatctcgc ccgcggggac cgtagccatc gctatcatcg
    14521 cgtaaacgcg gcagctcgcc agtgagtagt gagagataac gctcaaccgg ccataatcct
    14581 tgtaactgat taataaagat tacactgtct gcgttatttc ctaactgagg aacgaatccg
    14641 ggataactta accagcgtgg tacatcggga ttgtccccgg tcatacggcg gaacgtcgcc
    14701 atcgtgcgcc gctgcatggt ggggtcctgt acaacgatag ctgtgtgaac tcgttctacg
    14761 gcctgattca atagcgcgat gctaaagcgt gcgttttcac cgcagtttgt tgactggtct
    14821 tcaatccaga ttttttcatg cggaatatgc cagaactgat gagcgatatc cgccaggatg
    14881 gtcgcttctg ctctgccagt ggtgcggata gtgttgtagt gcggatgctg tgcgatggcg
    14941 ctatacaaaa aagtggtcga gtgaccgata ccaccactaa tcagtaaagg aatttgctga
    15001 tcgcgagcaa tcttacatgc cgcatcgata gtcggcataa ccgcatttcc tgcaagtatg
    15061 acgcaatcag cctgatacgg cacctcaccg gagaaatcat cctgcgccag ccactgtccg
    15121 ataacattta tggcatctat agttgccgtc gaaagcgtcg gaaacggggt aatgttcatg
    15181 gttcctcctt atcgttccct taagaatact gcgtgagata atagtgagaa gcggagcact
    15241 ctgaaaggaa gataatgctt tttctatctc gccatcgcga accagacact ggttttagtc
    15301 atacactatt tgcggagaaa ctgttgataa aacatcaata acatcatttg acgtggcaat
    15361 aacataatgt tcgatcattt tatttttatg aatttcacaa ctttgcatag taaaccatgt
    15421 aagataagtg gaattggcta ctctatagat ccccgtttgg ttaattccct gctttcctga
    15481 catacctaac aaatcacctt cgtcagtaac acgaaatgag tgtatccagt caaatgatat
    15541 cttaacttct gacttgatcg ccgggcatat cagggccagt tcaagcctgg cattcttata
    15601 gcttaaacct gacagatcgg cgtgtttaaa agacaacgaa gtcggcgtga tatttataaa
    15661 acgttcatcc atgttaacac ccttaatata tgagactgta ctcaatttaa ccttctacaa
    15721 aaatggtgtg agtctgttta agtaccatgt ataatcgata accgttgctg aattttaaaa
    15781 ttatcttgtc cagttattaa ccatttttca tgggaaacat ctgcaataca ttcattaatg
    15841 cgaaatacaa tttcccc
//
LOCUS       DAZFTN010000106        15587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFTN010000106 DAZFTN010000000
VERSION     DAZFTN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..647
                     /gene="yceJ"
                     /locus_tag="ACHKV2_003814"
     CDS             81..647
                     /gene="yceJ"
                     /locus_tag="ACHKV2_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6941619.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            651..1226
                     /gene="yceI"
                     /locus_tag="ACHKV2_003815"
     CDS             651..1226
                     /gene="yceI"
                     /locus_tag="ACHKV2_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO6941620.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1268..2320)
                     /gene="trhO"
                     /locus_tag="ACHKV2_003816"
     CDS             complement(1268..2320)
                     /gene="trhO"
                     /locus_tag="ACHKV2_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO6941621.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2545..3465
                     /gene="lpxL"
                     /locus_tag="ACHKV2_003817"
     CDS             2545..3465
                     /gene="lpxL"
                     /locus_tag="ACHKV2_003817"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO6941622.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDTPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3637..4863
                     /gene="mdtG"
                     /locus_tag="ACHKV2_003818"
                     /pseudo
     CDS             3637..4863
                     /gene="mdtG"
                     /locus_tag="ACHKV2_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            4946..5320
                     /gene="msyB"
                     /locus_tag="ACHKV2_003819"
     CDS             4946..5320
                     /gene="msyB"
                     /locus_tag="ACHKV2_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6941623.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5321..5548)
                     /gene="yceK"
                     /locus_tag="ACHKV2_003820"
     CDS             complement(5321..5548)
                     /gene="yceK"
                     /locus_tag="ACHKV2_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6941624.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5721..8264)
                     /gene="mdoH"
                     /locus_tag="ACHKV2_003821"
     CDS             complement(5721..8264)
                     /gene="mdoH"
                     /locus_tag="ACHKV2_003821"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO6941625.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8257..9792)
                     /gene="mdoG"
                     /locus_tag="ACHKV2_003822"
     CDS             complement(8257..9792)
                     /gene="mdoG"
                     /locus_tag="ACHKV2_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO6941626.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10185..11342
                     /gene="mdoC"
                     /locus_tag="ACHKV2_003823"
     CDS             10185..11342
                     /gene="mdoC"
                     /locus_tag="ACHKV2_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO6941627.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11350..12831)
                     /gene="clsC"
                     /locus_tag="ACHKV2_003824"
     CDS             complement(11350..12831)
                     /gene="clsC"
                     /locus_tag="ACHKV2_003824"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO6941628.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12773..13306)
                     /gene="ymdB"
                     /locus_tag="ACHKV2_003825"
     CDS             complement(12773..13306)
                     /gene="ymdB"
                     /locus_tag="ACHKV2_003825"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO6941629.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13401..13712)
                     /gene="ymdA"
                     /locus_tag="ACHKV2_003826"
     CDS             complement(13401..13712)
                     /gene="ymdA"
                     /locus_tag="ACHKV2_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO6941630.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13833..14165)
                     /gene="csgC"
                     /locus_tag="ACHKV2_003827"
     CDS             complement(13833..14165)
                     /gene="csgC"
                     /locus_tag="ACHKV2_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO6941631.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14224..14682)
                     /gene="csgA"
                     /locus_tag="ACHKV2_003828"
     CDS             complement(14224..14682)
                     /gene="csgA"
                     /locus_tag="ACHKV2_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO6941632.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(14723..15178)
                     /gene="csgB"
                     /locus_tag="ACHKV2_003829"
     CDS             complement(14723..15178)
                     /gene="csgB"
                     /locus_tag="ACHKV2_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO6941633.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3897 a         3975 c         3693 g         4022 t
ORIGIN      
        1 tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcaca
       61 aattcgtttt ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat
      121 ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt
      181 ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa ctacataaaa
      241 gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc
      301 caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca aaagctggac
      361 atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg
      421 ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg
      481 ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag
      541 tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca
      601 ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa
      661 gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg
      721 attacaaaat tgacaaagaa ggtcagcacg cctttgttaa ctttcgcatc cagcatcttg
      781 gctatagctg gttatatggc acctttaaag atttcgacgg tacttttacc tttgatgaaa
      841 aaaatccagc tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaatc
      901 acgccgaacg tgataaacat cttcgcagtg cagatttcct caataccgca aaatatccac
      961 aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg
     1021 atctgacgct gaatggtgta accaaacccg tcacgctgga agcgaaatta attggtcagg
     1081 gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa attaagctca
     1141 aggacttcaa tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt
     1201 cagtggaagg cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca
     1261 atgatattta ttctgttgga tcaggaatgc ccagtgttgt attcagacgt ccacgagact
     1321 tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg
     1381 gtaacgcgct ttcttcgcag caaatttcac tacaacaacc tttgtatttt tccgcacata
     1441 ctggacactg aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc
     1501 acggcgcacc gcactggtgg caatgcgcga taatctcatc agatatacgt tcgcccatcc
     1561 gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct
     1621 tacgggcgta ttcaataatt ccaccctcga tatgccagac tttattgaat ccgttatgtt
     1681 tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt
     1741 tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg
     1801 ccggaatttc cagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt
     1861 cgataaacag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact
     1921 caccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaacg atacgatcgc
     1981 ggaccttcat gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc
     2041 gtaagccatc taaagccgag tcgaaggcat agagctgcgc gcgaaatgtt ttaacattgc
     2101 tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa
     2161 cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt
     2221 ggaaatactt gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt
     2281 ttaacgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga
     2341 tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt
     2401 ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca
     2461 gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg
     2521 aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt
     2581 catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt cgtgcaattg
     2641 ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg
     2701 aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa
     2761 caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg actgatggaa
     2821 actggcatgg cgtggttctg gccggaccgc cgaatcgctc gctggacgga agtgatcggc
     2881 atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat
     2941 tttctgacac tggagctggg tgcgcggcag tttggtatgc aggaaccggg gattggcgtt
     3001 taccgcccga acgatactcc actgattgac tggctacaaa cctggggccg tttgcgctca
     3061 aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc
     3121 gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtccct
     3181 ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc
     3241 ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg
     3301 attatgctgc cgccagagtg ttctccgccg ctggatgatg ccgaaactac cgccgcgtgg
     3361 atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac
     3421 cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccatgccgga
     3481 tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct
     3541 ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta
     3601 tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc
     3661 cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggtgcc
     3721 gccttcagtc tggtaatgcc cttctaaccc ctctacgttg agcagcttgg cgttaccggc
     3781 cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc
     3841 atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact catgctatta
     3901 cgctctgctc tcggcatggg catcgtgatg gtgttgatgg ggctggcaca aaatatctgg
     3961 cagtttttga tcctgcgggc gcttcttggg ttacttggcg gatttgtccc caacgctaat
     4021 gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcactggg tacgctctcc
     4081 acaggcggcg ttagcggtgc gttgctcggc ccaatggctg gcggcctgct cgccgatagc
     4141 tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg ctttttcgtc
     4201 accctgtttt gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcacatg
     4261 cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcaccacg
     4321 ttaatcatcc aggtggcgac gggctcaatt gcccccattc tgacgctgta tgtccgcgaa
     4381 ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc
     4441 gtagctgctc tgctgagtgc accacgactc ggcaaacttg gcgatcgaat cggacccgaa
     4501 aagatcctga ttacggcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt
     4561 cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta
     4621 ctccccgccg tacagacact gttggtttac aactcgagca accaaatcgc cggacgtatc
     4681 ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt gatgggagca
     4741 gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta
     4801 ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac
     4861 tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc
     4921 tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga
     4981 cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag atgcgaatgt
     5041 gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga
     5101 gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg gtgaagccgc
     5161 gcaaagtgtt tttgatggcg actatgatga gatagagatc cgccaggagt ggcaggaaga
     5221 gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc tggataccga
     5281 ggaaggacgc gcagcagctg atgagtggga tgaacgttaa ttactcatac gggccatgat
     5341 gaatgtcgat cggcagcagt aaagtatcaa aaaccaggga gaatggcaga tcaaggattg
     5401 tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa tattggttgc
     5461 catgcccctg ccccggtata gtgcggctaa tgatgctgcc acagccactc agcaaggtca
     5521 ccatgatgct caccacaatt aaacgcattt gatacctctt ccgtaaaaaa ataccggcat
     5581 aacgccggta tttttattgg ctcgttgttt ttgccagatg tggcgtgaac gccttatccg
     5641 acctacaaaa tcttgcaaaa tcaacacatt gcaggagcga tgtaggcctg ataagcatag
     5701 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
     5761 ttgagcttta tcccttcgta ataactcacc catgaagaat atctctcagg ggaattccag
     5821 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcacaag gcgacgatcg
     5881 cgattcagct tctctggcgt ctcgttcagc gcctgttcaa catggcggtc acgggcgatt
     5941 tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat
     6001 gacgggttaa acactgcgtg cataaaacca tcatcaaggg agcgttgacg gttcatctca
     6061 aggaaccgat cggtatcaac cagtacctgc ggcggtgaat actcttccgg gatcaggaac
     6121 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
     6181 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccaac
     6241 cacgccatcc cgacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac
     6301 gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
     6361 ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
     6421 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccatattct
     6481 ttcgttcctt tacaccaaat aagcaaaatg ctcaatagct ttggcaggaa caacagcacc
     6541 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     6601 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     6661 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     6721 aggaataccg cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc
     6781 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggtaat
     6841 tcttcataag aacccgggag atcgtaagca atccagactc cccaacctgc acggcgcatc
     6901 aacgccgctt ccacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     6961 agcggggcca gtgcacagtg ctcgataaac ggtttcacgc ggataatcgc gttatgcccc
     7021 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     7081 acgcgggtcg cgaactgctg acagcgtgca tacagcgtat ccataccgga cgctttcggc
     7141 gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag gccgcacaaa
     7201 caatcaccgg tcattaccga gtcagcatcc agcaccacca tatagctgta ctggctgccc
     7261 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcgacg gcggcggcgg
     7321 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     7381 tctgcgacgc agatatccgg gttgtaactg tcactcagaa tgtagacatc aaagtgtttg
     7441 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     7501 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     7561 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagtag ttgcaggaag
     7621 cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc
     7681 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     7741 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     7801 taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     7861 gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     7921 tagcgcggtg ttacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     7981 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     8041 cgcccttcgt catctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     8101 agacgcgcct ttaccgagcc ttgcggggag tcatcctccc gcgcccaggt gcggtgttcg
     8161 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt
     8221 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     8281 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcgcgca tttcagtggt
     8341 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg tgaccgggtt
     8401 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     8461 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     8521 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     8581 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     8641 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     8701 gacgatgtta tcgttagttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     8761 accccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     8821 atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccaaagcctt gcgggttttc
     8881 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     8941 gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac gatagttatt
     9001 tgctggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     9061 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     9121 tggcataact acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat
     9181 ggttaaacgt ttatcagttg gttttggacg ctcgatccag aactctttga agcgtggaaa
     9241 ttcttcaccc gatggcaagg cggtatcaat tgccagaccg cgggcagaaa ggccataaac
     9301 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     9361 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     9421 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     9481 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     9541 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     9601 aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct
     9661 tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     9721 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     9781 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     9841 tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat
     9901 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     9961 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
    10021 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaactt
    10081 gaagcctgcg cctggcgcgg tcaggtcggt gtcgctatgg caacaagcct aaaggcataa
    10141 cccgacaggg ataagacgaa aaatccgaga ttagttaacc atatatgaat ccagtacccg
    10201 cgcaacgtga atatttcctc gactccatcc gcgcctggct gatgttgtta gggatccctt
    10261 ttcatatttc tttaatctat tcgagccata catggcatgt gaatagcgcc gaaccgtcat
    10321 tgtggctgac cctttttaat gacttcatcc actcgttccg catgcaggta tttttcgtta
    10381 tatccgggta cttttcctac atgctttttt tacgctatcc cttgaaaaaa tggtggaaag
    10441 tacgtgtcga acgtgtaggg atcccgatgt taacagccat ccccctactg acattgccgc
    10501 aatttattat gctgcaatac gtcaaaggga aagcggaaag ttggcctggg ctgtcattgt
    10561 atgacaaata taatacgttg gcctgggaat taatatcaca tctgtggttt ttactggtgt
    10621 tagtggtcat gacgacgctg tgcgtatgga tatttaaacg catcagaaat aatttagaaa
    10681 attctgataa aacgaataaa aaattctcga tggtaaaact atcggtgatt tttttgtgcc
    10741 tcggcatcgg ttatgcggta ataagaagaa cgatttttat tgtgtatcca cccattctga
    10801 gtaatggcac gttcaatttt attgtcatgc aaacgctatt ttatttaccg ttctttatcc
    10861 tcggcgcact ggctttcatt ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg
    10921 gctgtaccct cgctgcagca ttggcgtttg tcgcttattt actcaaccag cgctatggca
    10981 gtggcgatgc ctggatgtac gaaaccgagt cggtgatcac catggtcctc ggtctgtgga
    11041 tggtgaatgt ggtcttctcc tttggccacc gtttgcttaa cttccagtca gcgcgggtga
    11101 cttattttgt taacgcatcg ctgtttatct atctggttca ccacccgtta acgctgtttt
    11161 tcggcgcata cattacaccg cacatcacct ccaactggct tggttttctc tgtggcctga
    11221 tattcgtagt agggattgcg ataattctgt atgaaattca tttgcgcatc ccgttactga
    11281 agtttttgtt ctctggtaaa ccggttgtta agcgtgagaa cgataaagca ccagcccgtt
    11341 aagccacatt tacaataacc attccacggg caatatcgac gccagtctga ccataacccg
    11401 cttccagaaa ctggtagcgg gttctttttt gagaacaatc tctttctttg catgacgatc
    11461 aacccagttg atccgtcccc acctgtccag acggagctgc caggccgcat catactggct
    11521 ctgaataaag cgtttatcaa ttaactgagc cagcgtttcg ctctctatca cgaagcccat
    11581 ttcagtattg agcaatgttg aacgcggatc gaaattgaaa gaaccgataa acaccgtttt
    11641 accatcgatg ctaaaggttt tagcatgcag gctggcaccg gaattaccgg ttatgccgcg
    11701 atcgtgtaac gtactacttt gttcacgcgt cggcttgagt tcatataatt ccacgccata
    11761 gcggagcaat tttttgcgcc agcgcgcgta tccggcatgg acgacagcaa catcgttagc
    11821 ggcaagagaa ttggttagga tcgcaatctt tacccctttt ctcaccatcc gtaagagttg
    11881 cgccacacct gcgcgtgtcg gtacaaaata ggaagagata atatcgatgc gttcactggg
    11941 tgagcccatg atatcgaaca ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc
    12001 ccctttcgcc ggatcatcac ttaataaacg tgttttcgcc cagataagcg gcaacgttcc
    12061 atcaaccaga tgatttataa acggactgga ttccattttg cgtaagtaac gatgcgtcat
    12121 ggcatcgtta tgccaggagg cgggtaactc gatgcgatcc gccatttcac cttccgggac
    12181 atccagcacc tgctgtaagg gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc
    12241 ggcaacgtcc tctactacgg gtcctattgc catgacatct aaatccgaaa aaagtggctc
    12301 ttcccctgcc ccaaaatagg catcaccaat atttcgcccc cctaccaacg tcaccacgcc
    12361 atcgacagtg aaacttttat tgtgcatacg gcgattgaga cgggaaaagt cggtgatata
    12421 accaagcgga cgcagcaagc gaaacgagaa aggattaaaa agccggactt caatgcgggg
    12481 atggctgtca agcaagcgta aaatgtcgtc aagtccgggc gtattgttgt cgtccagcaa
    12541 caaacggacg cgaacgccac gctttgctgc ggctaacagg gcggaaaaca gtaaccgacc
    12601 cgacatatcg tcctgccaga tgtaatactg aacatccagc gtatgctctg acatttcggc
    12661 cagacgatac cgggcggcaa acgcatccag acttttctcc agagggaaaa gaccacactg
    12721 accgggatgt tgcgaacaca gtggcagcac cgcgctcgcc agccggggca aatcattcat
    12781 ctccttgttg ggtaaggagt ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa
    12841 agtatacctg ttcgggtaaa gcgtgacggg taataaattc tgaaacggtt tttactgcga
    12901 tttcagccgc tgccgcacga gggtaaccat aaaccccagt actgattgca ggaaaagcca
    12961 ctgacgtata gctgtttgcc gccaccagtc gtaggctatt gagataggca tcctgcaaaa
    13021 gctggtcttc gttttgttca ccaccacgcc agactggccc gacggtgtgc actacggctt
    13081 tagcaggaag atcgcctgca agcgtaataa cggcatgccc cgtagggcaa tcgccctgct
    13141 gttgcctgac ttttaaacaa gcatccagca gggccggacc cgctgcgcga tgaatggccc
    13201 catcgacgcc gccacctccc attaatgaag gattagccgc attcacaatc acatcaacgg
    13261 ccagtttggt aatatcaccc tgcacaacat gaatacgcgt tttcataacg cctccttaca
    13321 ccttcttgtt tcttaagtgt acagcaggct ttttggttaa atcgcatttt tctgctcagc
    13381 aacgtgagtt gttcagaaac ttagttatat tcgatgttca ttacagccag ctttttctgt
    13441 ttatcaagat actgcacttt tactgaggct atttgcgcgt ttttgacatt gccgctcatt
    13501 aacgctttgc tggagtaggt tttacccgga acagaaccat tctgtgggca gttcatttct
    13561 acgggtgatg actgggtgct gacgtcacat gccggttcca caatttggcc ataaaaatgg
    13621 atcacgcccc cttgcgcggt gcttcctgcg atggcaataa aaggaaaaaa caaactgccg
    13681 agcataagag agtttaaaaa aggtctgaac atttattcta tttcctcaat gattagtcat
    13741 ccttgagggt tgtgttatcc atactttcag gttggctgat tcgcagcaga ccattctctc
    13801 cagattcatc ttatgctcga tatttcaaca aattaagact tttctgaaga gggcggccat
    13861 tgttgtgata aatgaagtga ctgtccatca gaaacagtaa caactatttt cacccgatcg
    13921 tccggggaaa tatttaaact caacttcgtc aaagcaatgg gttgattagc aggcaatgaa
    13981 agggtctttt cttgcttcgt ctgactttgc cctgaactgc cttcgcgcag ggacaatatt
    14041 tgtactctgc acagacaaga ttgagtaaga gtgacttcag gaataatggt gtacatatcc
    14101 ccttgctggg tcgtattaaa ggttatctga ctggaaagtg ccgcaaggag taataacgca
    14161 ttcatattct tctcccgaaa aaaaacaggg cttgcgccct gtttctttaa tacagatgat
    14221 gtattagtac tgatgagcgg tcgcgttgtt accaaagcca acctgagtta cgttgacggt
    14281 ggaattagat gcagtctggt caaccgctgc accgttgccg ccaccgaatt gtttaactgt
    14341 catatgagag tctttaccgt tccactgatc aagagtggcg ctgttaccaa agccacgttg
    14401 ggtcagatcg attgagctgt catctgagcc ctgaccaaca tctgcaccgt taccaccacc
    14461 atgctgggta atagtaagat cagagttacg agcatcagct tgcagagcaa gtgcagagtt
    14521 accaccaccg tactgataaa tattcagctc tgaattcggg ccgctgttat taccgccacc
    14581 accgtggtta ccgccaccgc cgccgtactg aggaacaaca cctgccagag cgctaccgga
    14641 gaatacgatt gctgcaattg ctgctacttt taaaagtttc atgtaaaacc cccatcggat
    14701 tgatttaaaa gtcgaatgga aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc
    14761 tctgcactac aattgccgtt ttttgagtac catactgtgt aatatttgct ttattaccag
    14821 aacctttctg gataatcatc gcagtattac cataagcacc ttgcgaaata ctggcatcat
    14881 tggcactgcc cgcctgatca atatatgcaa ggttataatc tcctgtctgg tcaatctttg
    14941 cccggttgct actaccttct tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta
    15001 actgagcact attattagtc ccagcttgac caattatggc tgcctgatta aatgaagact
    15061 tactcaattc atttaccgcg aagttatatt ctgaattagc taaatcataa cctgctgcgg
    15121 ctgcaatccc aggcgcaccc agtattgtta acatcataaa taacaatttg tttttcatgt
    15181 tgtcaccctg gacctggtcg tacatttaag aaattaaatc atttcaactt ggttgttaac
    15241 gcaacctata ttttgttaac gctgcgttac gatggaaagt atgtctgcgg aaatattttt
    15301 aataactcac ccgcgcgttg tatttttctt tttttgttca taccaaagta ttaaaaccca
    15361 cgtaaatacg ctgttatcta cgcaaaaaat attttgtttt cttttaaatc tccgttttcc
    15421 gctagcaaaa aacaccagac agtcattctt cttgcccgtc gctgattgct gcgatatgtc
    15481 ttgcgcacaa gccgtgacaa acctcgtctt tttgcagtaa aaaattgtcc acggtggtgt
    15541 ggagaaaaaa caagaacgtt ttacatgacg aaaggactac accgaaa
//
LOCUS       DAZFTN010000107        15542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFTN010000107 DAZFTN010000000
VERSION     DAZFTN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..328
                     /locus_tag="ACHKV2_003830"
     CDS             125..328
                     /locus_tag="ACHKV2_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO6941634.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHKV2_003831"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHKV2_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHKV2_003832"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHKV2_003832"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHKV2_003833"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHKV2_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO6941635.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2484..2559
                     /locus_tag="ACHKV2_003834"
     tRNA            2484..2559
                     /locus_tag="ACHKV2_003834"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2517..2519,aa:Asn,seq:gtt)
     gene            2873..10855
                     /locus_tag="ACHKV2_003835"
     CDS             2873..10855
                     /locus_tag="ACHKV2_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFO6941636.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDFSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11117..12169)
                     /locus_tag="ACHKV2_003836"
     CDS             complement(11117..12169)
                     /locus_tag="ACHKV2_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO6941637.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12483..13799
                     /gene="shiA"
                     /locus_tag="ACHKV2_003837"
     CDS             12483..13799
                     /gene="shiA"
                     /locus_tag="ACHKV2_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO6941638.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13901..15355
                     /gene="amn"
                     /locus_tag="ACHKV2_003838"
     CDS             13901..15355
                     /gene="amn"
                     /locus_tag="ACHKV2_003838"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO6941639.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4260 a         3697 c         3721 g         3864 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     2461 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2521 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     2581 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     2641 atcttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     2701 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacatcca
     2761 aaactgccta acgaaaagtt attaattttc aatcatattg ctatcaggat ttacattttt
     2821 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     2881 gaagaagaga agtggagaag aaataaatga ccgacaaatc ttatgcggga tgggaattaa
     2941 actacgccgc ttaactgcgg gtatctgcct gataactcaa cttgtgttcc ctatggctgc
     3001 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     3061 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     3121 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     3181 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     3241 aaataattta accccgccac cgggtaatag cagtggcaac cttgagcaac agatagccag
     3301 tacttcacag caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa
     3361 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     3421 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     3481 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     3541 tctccatcgt actgacgagc gtacgcagat taacaacggc ttgggttggc gtcatttcac
     3601 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     3661 ccgcgccggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     3721 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc
     3781 ggccaatggc tgggatgtac gcgcagaagg ctggctaccc gcctggccgc accttggcgg
     3841 taaactggtc tatgaacagt attatggcga tgaagtggcc ctgttcgata aagatgatcg
     3901 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     3961 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     4021 ttttacctgg caacctggaa gcgcgatgca gaaacagctt gacccgaatg aagtcgatgc
     4081 acggcgtagc cttgcaggca gccgttttga tctggtggat cgcaacaaca acatcgttct
     4141 ggaatatcgc aaaaaagaac tggttcgcct gaccctgaca gaccccgtga cagggaagtc
     4201 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     4261 cgtcgaagcc accgctctgg aagctgccgg tggtaaagtg gttacaacgg gtaaagatat
     4321 tctggttacc ctgccggcgt accggttcac cagtacgcca gaaaccgata acacctggcc
     4381 gattgaagtc accgctgaag atgtcaaagg caatttttcg aatcgtgaac agagcatggt
     4441 agtcgttcag gctcctacgc taagccagaa agattcctcg gtatcgttaa gtagccagac
     4501 gttgagcgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     4561 taatcctgtt atcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     4621 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     4681 gtctggcacg ctgacgctga tgccacagct gaacggtgtg gatgcggcta aagcccccgc
     4741 cgtggtgaat atcatttctg tttcgtcatc ccggactcac tcgtcaatta agattgataa
     4801 ggaccgttat ctctccggga atcctatcga ggtgacggta gaactgagag atgaaaatga
     4861 caaacctgtt aaggagcaaa aacagcaact gaataccgca gtcagcatcg acaacgtgaa
     4921 acctggtgtc actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac
     4981 cgcctatacc aaaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga
     5041 tttgcatacc gctggattta tcatcgacgc caacccgcag tcagcgaaaa ttgcgacatt
     5101 atctgccagc aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa
     5161 tgtcgctgat gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag
     5221 cggatcggca acttccttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc
     5281 gacttttgat ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa
     5341 tggcgtgaaa caaacgttaa tcgtcagttt tgtcggcgac tcgagtaccg cgcaggttga
     5401 tctgcagaag tcgaaaaatg aagtggtcgc tgacggcaat gacagtgcca caatgaccgc
     5461 gacagttcgg gatgcaaaag gcaacctgct caatgacgtc aaggtcacct tcaatgtcaa
     5521 ttcagcagca gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc
     5581 tacgctgacc agtttgaaaa atggtgatta tacggttacg gcctctgtga gctctggttc
     5641 tcaggctaat caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag
     5701 tgtgccttca ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt
     5761 gcaggataaa aatggcaatc cactaaaaga taaagaaatc accttctctg tgccaaacga
     5821 cgtcgcaagt cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac
     5881 tgcaatcgcc tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc
     5941 taacagcaat gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt
     6001 tgtcgttctg caaacatcga aagcggaaat cattgggaat ggcgtggatg agacgactct
     6061 gacagcaaca gttaaagatc cttttgataa cgtggttaaa aatctttcag tagtcttccg
     6121 cacctccccc gcagacacgc aactgagtct gaacgcgcgt aatactaatg agaacggtat
     6181 tgccgaagtt acccttaagg gcacggtttt gggtgttcat acagccgaag ccatactgct
     6241 taacggcaac agagatacga aaatcgtcaa tattgcgccc gatgccagca acgcgcaggt
     6301 caccctgaac atccctgcac aacaggtggt gacgaataac agtgacagcg tgcagctgac
     6361 ggcgacggtg aaagacccgt cgaatcatcc ggtggcggga ataacggtga acttcaccat
     6421 gccacaggac gtggcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc
     6481 caatggcgaa gcgcatgtca ccctcaaagg caaaaaagcg ggcacgcata ctgtgaccgc
     6541 cacgctgggt aacaataatg ccagcgatgc gcaaccagtc accttcgtgg cggataagga
     6601 cagcgcggtt gtcgttctgc aaacatcgaa agcggaaatc attgggaatg gcgtggatga
     6661 gacgactctg acggcaacag tgaaagatcc ttttgataac gcagtaaaag atctacaggt
     6721 caccttcagt accaaccccg cagatactca acttagtcag agcaaaagca atactaacga
     6781 cagtggtgtg gccgaagtta cctttaaggg cacggttttg ggtgttcata cagccgaagc
     6841 cacactgcct aacggcaaca acgatacgaa gatagtcaat attgcgcccg atgccagcaa
     6901 cgcgcaggtt acgctgaaca tccctgctca acaggtggtg acgaataaca gcgacagcgt
     6961 gcagctgacg gcgacggtga aagatccgtc gaatcatccg gtggcgggaa taacggtgaa
     7021 cttcaccatg ccacaggacg tggcggcaaa ctttaccctc gaaaataacg gtattgccat
     7081 cacccaggcc aatggggaag cgcatgtcac gctcaaaggt aaaaaagcgg gtacgcatac
     7141 ggttaccgca acgctgagta ataacaatac cagtgattca cagccggtaa cgtttgtggc
     7201 ggacaaaacc tcggctctgg ttgttcttca gatatcaaaa aatgagatca caggtaatgg
     7261 cgtcgatagc gcaacgctaa ctgcaacggt caaagatcag ttcgacaatg aggtgaacaa
     7321 tcttccggta acattcagca cagcttcttc aggcctcacc ctgaccccag gggaaagtaa
     7381 taccaatgag tctggcatcg cgcaggccac tctcgcaggc gttgcctttg gtgagcagac
     7441 ggtcactgca tcactggcta ataatggtgc cagcgacaac aaaactgtgc attttattgg
     7501 cgacacagcg gcggcaaaaa ttatcgagtt gacgcctgtc ccagacagca taatcgcagg
     7561 taccccgcag aacagctccg gcagcgtcat caccgccaca gtcgttgata ataatggctt
     7621 tccggtgaaa ggtgtgactg tgaacttcac cagcaacgca gcgacagccg aaatgacgaa
     7681 tggcggtcaa gccgtgacga acgaacaggg taaggctacc gtcacttata ccaatacccg
     7741 ctcctcgata gaatcaggag cgagaccgga taccgttgag gccagtctgg aaaatggtag
     7801 ctccacgctt agcacatcaa ttaatgtcaa cgctgatgcg tctacggcac atctcacctt
     7861 gctacaggca ctttttgata cagtctccgc aggcgacact accaatctgt atattgaggt
     7921 gaaggataat tacggcaacg gagtacccca gcaggaggta accctcagcg tttcaccaag
     7981 tgaaggtgtg acccccagta ataacgctat atatacgacc aatcacgacg gcaattttta
     8041 cgcaagcttt accgctacaa aagccggggt ataccaagtg acggcaaccc tcgaaaatgg
     8101 cgattcgatg caacaaacag tgacctatgt gccgaacgta gcgaatgctg aaatctcgct
     8161 ggcagcctcg aaggatccgg taattgccaa caataacgat ctcacgacac taacagcaac
     8221 agtcgctgat acagagggca atgcgatagc caacagtgag gtaacattta ctctgccgga
     8281 agatgtgagg gcgaacttca cgctgggcga tggcggtaaa gtggttactg atactgaagg
     8341 caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct catactgtta cagcatcgat
     8401 ggctggcggt aagagtgagc agttggtggt gaactttatt gcggatacac tcactgcgca
     8461 ggttaatctt aacgttaccg aggacaattt tatcgctaat aacgtcggga tgaccaggct
     8521 gcaggcaaca gtgactgatg gaaacggcaa cccgttagcc aatgaggcgg tgacattcac
     8581 gctaccggca gatgtgagcg caagctttac tctcggacaa ggcggttccg ccattactga
     8641 catcaacggc aaggctgaag ttacactgag cggtacaaaa tccggcacct accccgtgac
     8701 agttagcgtg aacaattatg gtgtcagtga tacgaaacag gtgactttga ttgccgatgc
     8761 tggtaccgca aaactagcct ccttaacctc tgtatactca ttcgtcgtca gcacgaccga
     8821 gggcgcgacc atgactgcaa gcgtcactga cgctaacggc aacccggtag aaggtataaa
     8881 agttaatttc cgcggaactt ccgtcacgct aagcagcacc agcgttgaaa cggatgatcg
     8941 gggtttcgct gaaattcttg tgacaagcac cgaggtcgga ctgaaaacag tttcagcctc
     9001 tctggcagat aaacctactg aagtcatctc gcgattactg aatgcaaaag cagatattaa
     9061 ttctgcaacg attaccagtc tggagatacc tgaaggtcag gtcatggtcg cacaagacgt
     9121 agcagttaaa gctcacgtca acgaccagtt tggcaatccg attcttaatg aatctgtaac
     9181 attcagtgca gaaccaccag agcacatgac catcagccaa aatattgtct ctactgatac
     9241 gcatggtata gccgaggtca ctatgacgcc cgaaagaaac ggttcgtata tggtgaaagc
     9301 atccctggcg aatggatcct cttatgagaa ggatctggtg gtaatcgatc aaaaactgac
     9361 actctcggcg tccagcccgc ttatcggtgt caattcccca acaggtgcaa ctctgacggc
     9421 aacgctaact tctgcaaatg gcactccagt ggagggtcag gtcatcaact ttagcgtaac
     9481 gccagaaggt gcgacgttaa gtggcggaaa agtgagaacc aactcttcag gtcaggctcc
     9541 agtcgttctg accagcaata aagtcggtac atatacggtg actgcatcgt tccataacgg
     9601 cgtaacaata cagacacaga caatcgtgaa agtcactggc aactcaagca ccgcccatgt
     9661 tgctagcttt atcgctgatc catcgactat agccgccacc aacagtgatt ttagtacctt
     9721 aaaggcaacg gttgaggatg gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc
     9781 cttaaaaagc ggctctgcca cattaacgtc attaacagcg gtgacagatc aaaacggaat
     9841 cgcgacaaca agcgtgagag gagcgataac ggggagcgtc acggtaagcg cagtcacgac
     9901 cgctggtgga atgcaaacag tagatataac gctggtggca ggcccggcag acgcctcgca
     9961 gtccgtcctt aagaacaatc ggtcatcatt gaaaggagac tttaccgata gtgctgagct
    10021 acatcttgtt ctgcacgata tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt
    10081 tgtgcagtca ggtaccaacg cgccctatgt gcaagttagt gcaattgact acagtaaaaa
    10141 tttctcaggc gagtacaaag ccactgttac aggcggcgga gagggtatcg caacgctgat
    10201 ccctgtattg aatggtgttc atcaagcggg tctgagtacc acaatacaat tcactcgcgc
    10261 agaagacaaa ataatgagcg gtacagtgtt agtcaatggt gctaacctac cgacaactac
    10321 attcccttcg caggggttca ctggggcgta ttatcagttg aataatgaca actttgcccc
    10381 aggaaaaacg gcggctgatt atgagttttc aagctctgcc tcctgggttg atgttgatgc
    10441 taccggtaaa gtgacattta aaaatgtcgg cagcaaatgg gagaggatta cggcgacgcc
    10501 aaaaacaggc ggccctagct atatatacga aatccgagtg aagagttggt gggtgaacgc
    10561 cggcgatgct ttcatgatat acagccttgc tgaaaatttt tgcagtagca atggctacac
    10621 acttcccctt ggagaccatt taaaccatag tcgttcccga ggcatcgggt cactgtacag
    10681 tgaatgggga gatatggggc attacacgac tgaagctggt tttcattcaa atatgtattg
    10741 gtcatcgagt cccgcaaact caaacgaaca atacgtagtt tccctggcaa caggtgatca
    10801 aagcgtattt gaaaagcttg ggtttgctta tgcgacatgt tataaaaacc tctgattttc
    10861 cgttataatt tcttaaagag tgctccattc ggggcactct tttttacatt ccctctatag
    10921 agcttgccct tctctcacag aaaatgaata aataaaaatg cgataccgca agaatcgcgt
    10981 tcgatgtgac tatgatcgta tccggtgagt acagctttta tccaaaggct ggttttctgt
    11041 gatgattgac cagcctcaga gtattgatga caccaccgtt cataacagat cggcattact
    11101 atgcataagt actgtctcat attctcgcca aaacactttt taatgccttt gcgaattcat
    11161 ccagacttat attgtcaatt ttttgcccaa cgacgactga ctccgaaaca tcggccattg
    11221 cgggccatcg cgttttgtaa tcagcataaa ttgcaacaac gggggtatta taggcacttg
    11281 ccatatgtaa tattgaggtg tcaggagtta ttgcgatata agcatctttt atgattgctg
    11341 ctgaacgttt aatcgagggt gataaggata aacggtagac attattataa caatccacta
    11401 ataccctggc tttatcttcc ccttttggtc catgaacgat cactattggt atatctgttt
    11461 cactttgaat tttttctatt agattttctg caatcgatag tgaaaatgta cgatcctgcg
    11521 agctaccttc taaattaagc gcaatgtaag atcctaacga tcgcatttcc tcgcgcacct
    11581 catcgagtac atcctcactt agtggcaatt catatattgg cctgactgct ggaaaacccg
    11641 cctcacgcat cataaatgcc catgtcatgg gtacgggagc ccggagtttc tgatccatcc
    11701 gggacgcgtt cttacacaca ggggaatagc acttcatggt taaaccaaca acctgaaaat
    11761 tcgtttttgc tttcaactga ctgataaata acatcgtttt caggttcttt ttacgcatcg
    11821 cctctataca aagatccggc gtaccgtatt gctgtgttat gttcttcgct aaatctttta
    11881 tttcttttaa tgttgcgtga tcctgcatag tcattgtgac taatgttaat ttagtctgtt
    11941 caagtttgag cgcattaaag acttctaaat taattgtcga cgtaacaatt aatagatgct
    12001 taattttatg caattcaagc gcccgaataa caggaaagat ggccatagca tcgccaatct
    12061 gatcgggaat atggatgaca acaaagtctg ttttttcaat attgaaatta taagctttat
    12121 aatcgtagta actaaatgca atacgtctca acaatgatac taaaaacata actaacctcg
    12181 cctccctact ggttataatg caatgcagtc tatcagaatc atcagggtgc aattttgtgc
    12241 atatgcggac ttttatgttt catatctcga acctgtgggg cctctgctta atccttaaac
    12301 aacaccagca actcctgcgc tttcatcttc cagcgaattt ttcatgttgc cgttaatcag
    12361 ccataaaatc atttgcagat gcgctctgtc gaggtagtct cataaggttc gtttatagat
    12421 cgacgcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
    12481 ttatggactc cacgctcatc tccactcgtc ccgatgaaag gacggtttcg ttaagtcgcg
    12541 cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac
    12601 tctatggcat taccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
    12661 ctatgggaac gctcgccgca tttgccacct ttggcgtcgg attccttttc cgtccgctcg
    12721 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
    12781 ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
    12841 ttgggtggtg ggcacctatt ttactggtga cgctgcgtgc cattcaggga tttgccgtcg
    12901 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
    12961 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
    13021 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagctgg ggctggcgca
    13081 ttcctttcct gtttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg
    13141 aagagtccgc ggaatttgaa caacagcaat ataatcaagc ggccgcgaaa aaacgcatcc
    13201 cggttatcga agcgctgtta cgacatcccg gtgctttcct gaagattatt gcgctacgac
    13261 tgtgcgagtt gctgacaatg tacatcgtta ccgcctttgc acttaattat tcaactcaga
    13321 atatggggtt accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct
    13381 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata
    13441 tcacaggcgc gttgatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
    13501 aatctatttt ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg
    13561 tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
    13621 gtgctggagt cggttatcag gttgccagtg tggttggcgg tggatttaca ccttttattg
    13681 ccgctgcgct catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg
    13741 ctggatgtct gatttccgca atgaccgctt tgttgatgaa agacaatcaa cgcgcttgat
    13801 agtctggcga agatcatccg atcttcgcct tacacttttg tttcacattc ctgtgacata
    13861 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
    13921 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
    13981 attacgcaac gccattggca actatattac aagtggcgaa ttacctgatg aaaacgcccg
    14041 caaacaaggt ctttttgtct atccatctct gaccgtaacc tgggacggta gcacaacaaa
    14101 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
    14161 tactcgccct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
    14221 ttatggcgcg catatctcgg tgcaaccctc acagcatgaa atcccttatc cttatgtcat
    14281 cgatggctct gaattaacac ttgatcgctc aatgagcgct gggttaactc gctatttccc
    14341 gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaactga
    14401 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg
    14461 ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac
    14521 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
    14581 ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
    14641 agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac
    14701 cgccgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac
    14761 catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg
    14821 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
    14881 tgaccacgtt cttgatgcgg ttctgccacc cgatattcct attccgagca ttgctgaagt
    14941 gcaacgtgcg ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa
    15001 acagcggcta cgtactggta ctgtggtaac cacagatgac aggaactggg aattacgtta
    15061 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
    15121 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
    15181 agataaaccg ttgcatggcg agattaaact tcccggtcag gctaaccgtt tttatgaagg
    15241 cgctatttcc gaacacctac aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
    15301 cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt
    15361 tctgaacctt cttatcaaaa atagatgtga aggattaagt aagacatctg gcaaatagcc
    15421 cgcaataaac gcgggctgtt tgcttccttg ggcggatacg agttttatta tcgtcttaat
    15481 gatttccaca tattaaaagc aagtatgctt tcaaaacaca attataaaaa atcccgccaa
    15541 ca
//
LOCUS       DAZFTN010000108        15535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFTN010000108 DAZFTN010000000
VERSION     DAZFTN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..346)
                     /locus_tag="ACHKV2_003839"
     CDS             complement(65..346)
                     /locus_tag="ACHKV2_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFO6941640.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(608..1717)
                     /gene="apbC"
                     /locus_tag="ACHKV2_003840"
     CDS             complement(608..1717)
                     /gene="apbC"
                     /locus_tag="ACHKV2_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO6941641.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1849..3882
                     /gene="metG"
                     /locus_tag="ACHKV2_003841"
     CDS             1849..3882
                     /gene="metG"
                     /locus_tag="ACHKV2_003841"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO6941642.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4023..4751
                     /locus_tag="ACHKV2_003842"
     CDS             4023..4751
                     /locus_tag="ACHKV2_003842"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFO6941643.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6708..7832
                     /locus_tag="ACHKV2_003843"
                     /pseudo
     CDS             6708..7832
                     /locus_tag="ACHKV2_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7845..10830
                     /locus_tag="ACHKV2_003844"
                     /pseudo
     CDS             7845..10830
                     /locus_tag="ACHKV2_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10840..14469
                     /gene="yehI"
                     /locus_tag="ACHKV2_003845"
     CDS             10840..14469
                     /gene="yehI"
                     /locus_tag="ACHKV2_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO6941644.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14531..14848
                     /gene="yehK"
                     /locus_tag="ACHKV2_003846"
     CDS             14531..14848
                     /gene="yehK"
                     /locus_tag="ACHKV2_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO6941645.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         4169 a         3847 c         3751 g         3768 t
ORIGIN      
        1 aagccgaaat cattcatagg aatgattttg gctttttaaa tatgcttaaa acggtgttgg
       61 caaattaatc gtagcgattt aacacgattt tgcgtttgtc gttattaccc acagctacca
      121 cttctgtcgt aacgcctttt gcagtagaag aagcacgata gttggtttca actcgttgta
      181 acaactgccc acaacgttgc tgaagatgat tattaacaac atccatactg catgtagggg
      241 aactaattct gccattgatg gttaatgttg tagtatcaga agagaacgcg ggtgatgcta
      301 gtccataagc aagaaaaaca gctcctgaca accaatactt attcattgaa cgttatccct
      361 gtagtaaagg ttatgccggg tagattgagt gcataacaca ctatagctgt acatccgcta
      421 cacagccacg agggatgata aagagacagt ggttgcacaa tcaatcgcct ttgtacagtt
      481 tcggactaag atccactgaa tattcgcagt attaaataag tgttcattaa tgcggcaaaa
      541 acaaatacac cgcatcaata taacatctct gcggtgtatg aagaataacc agaatggtta
      601 ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat gacttcaccc tgccagtaga
      661 gctgagctgc aacgcggtca gccagctggc ggtagatagc ggtaaattcg ctctccggac
      721 ggctgataac cgttggcgtg cctttatcga gatcctcacg taaggagatg tgtaacggca
      781 tctgccccag cagctgtgta tggtattttt cagccagttt ctctgcacca ccggtaccga
      841 aaatcggttc atggtgacca cagttactgc aaatatgcac actcatgttc tcgacaatac
      901 cgagcaccgg aacctcgact ttctcgaaca tcacaatgcc ttttttcgca tcgatcaacg
      961 cgatgtcctg cggggtggtg accacgactg caccagttac tggaatgttt tgcgccagcg
     1021 tcaattgaat gtcaccggtg cctggcggca tatcaagcac cagatagtcg agatccggcc
     1081 acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc catcggtcca cgccacacca
     1141 tcgcattgtc gtcagtgacc agataaccaa ttgaattggt tgccaggcca tgagacataa
     1201 tcggtgccat atgggtaccg tccggcgaag ttggacgctg attttctgcg cccagcatgg
     1261 tgggaattga tgggccgtag atatcggcgt ccagaatgcc gactttcgcc ccttccgccg
     1321 ccagtgccag cgccaggttt accgccgtgg aggatttacc cacgccgcct ttaccggaac
     1381 tgaccgcaat gatgttcttc acgccattaa tacccggctg attcttcacg cgttttaagg
     1441 tggcgatatt gtgcgacagt ttccagtcga tagctttcgc cccggtgata cgcagcagtt
     1501 cggcgctaca ttgctctttt aactcttcaa acgcgctatg ccagacgaat ggcataacca
     1561 gttccacatg caacgtgtcg tccatccagg cgacatggtg caacgctttc agcgtggtga
     1621 ggttatgctt cagggttggg tgctgaaaat tggccagtgt cccggcgacc attgcgcgca
     1681 aggcttctgg tgatttggcc tgggattgtt cgttcatccc gactcctttt ttgtatagat
     1741 aaaccatcag ctgatagttt acctgaagaa tatagagaag tacttactta acattttccc
     1801 atttggtact atctaacccc ttttcactat taagaagtaa tgcctactat gactcaagtc
     1861 gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat ccacctcggc
     1921 catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat gcgcggccac
     1981 gaggtcaact tcatctgcgc cgacgatgcc cacggtacac cgatcatgct gaaagctcag
     2041 cagcttggta tcaccccgga gcagatgatt ggcgaaatga gtcaggagca tcagactgat
     2101 ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga agagaaccgc
     2161 cagttgtctg agcttatcta tactcgcttg aaagaaaacg gttttattaa aaaccgcacc
     2221 atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc
     2281 acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt ctgcggcgcg
     2341 acctacagcc cgactgaact gatcgagccg aaatcggtgg tttctggcgc taccccggta
     2401 atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat gttgcaggca
     2461 tggacccgca gcggtgcgtt gcaggagcag gtggcaaata aaatgcagga gtggtttgaa
     2521 tccggtctgc aacagtggga tatctcccgc gacgcgccgt acttcggttt tgaaattccg
     2581 aacgcgccgg gtaaatattt ctacgtctgg ctggatgcgc cgattggcta tatgggttct
     2641 ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa
     2701 gactccaccg ccgagctgta ccacttcatc ggtaaagata ttgtttactt ccacagtctg
     2761 ttctggccag caatgctgga aggcagcaac ttccgcaagc cgaccaacct gtttgttcat
     2821 ggctatgtga cggtgaacgg cgcgaagatg tctaagtctc gcggtacctt tattaaagcc
     2881 agcacctggc tgaatcattt tgacgccgac agcctgcgtt actactacac tgcgaaactc
     2941 tcttcgcgca ttgatgatat cgatctcaac ctggaagatt tcgttcagcg cgtgaatgcc
     3001 gatatcgtta acaaagtggt taacctggcc tctcgtaatg cgggctttat caacaagcgt
     3061 tttgacggcg tgctggcaag cgaactggct gacccgcagt tgtacaaaac cttcactgat
     3121 gccgctgaag tgattggtga agcgtgggaa agccgcgaat ttggtaaagc agtgcgcgaa
     3181 atcatggcgc tggctgatct ggctaaccgc tatgtcgatg aacaggctcc gtgggtggtg
     3241 gcaaaacagg aaggccgcga tgctgacctg caggcgattt gctcaatggg catcaacctg
     3301 ttccgcgtgc tgatgactta cctgaagccg gtactgccga aactgaccga gcgcgcagaa
     3361 gcatttctca atacagaact gacctgggat ggtatccagc aaccgctgct gggccacaaa
     3421 gtgaatccgt tcaaggcgct gtataaccgc atcgatatga agcaggttga agcactggtg
     3481 gaagcgtcta aagaagaagt gaaagcagct gccgcgccag ttactggccc actggcagat
     3541 gatccgattc aggaaaccat cacctttgac gacttcgcca aagttgacct gcgcgtggcg
     3601 ctgattgaaa acgcagagtt tgttgaaggt tctgacaaac tgctacgcct gacgctggat
     3661 ctcggcggtg aaaaacgcaa tgtcttctcc ggcattcgtt ctgcttaccc agatccgcag
     3721 gcactgattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa aatgcgcttc
     3781 ggtatctctg aaggcatggt gatggctgcc ggtcctggcg ggaaagatat tttcctgcta
     3841 agcccggatg ccggtgctaa accgggtcat caggtgaaat aatccccctt caaggcgctg
     3901 catcgacagc gccttttctt tataaattcc taaagttgtt ttcttacgat tttgtctctc
     3961 tctaacccgt ataaatactg gtagcatctg cattcaactg gataaaatta cagggatgca
     4021 gaatgagaca ctttatctat caggacgaaa aatcacataa attctgggcg gttgagcagc
     4081 agggaaacga gctgcatatc aactggggca aagtcggcac taacgggcaa agccagataa
     4141 aaagttttgc ggatgttgca gcagcggaaa aagcgaaact gaagcttatc gcggagaaaa
     4201 cgaaaaaagg ctatgtggaa aatgcttcag caaacgtgca tatccccccc atcaccaaag
     4261 ccactcctga agttgaaact tcccctgaga gtaaaaacca acgcccgtgg ctggctgatg
     4321 atgcagttat tccggtaact gacgatatta accgatttgc ttttcctcat cgctctcgcc
     4381 ccagagagat caattatctg cgcaaagacg gtgagatatg gaagcgtatt gcagataaca
     4441 ccagggcata tgatcccgac aacaattacc gctcttatcc tgaaaactgg cagcaggctt
     4501 ttgctgagtt acaaaggcga attctggata atcaacagac agggagtgcg caatctgatg
     4561 cagcattgct ctggagtttt tggaactctt actctgcaga tgaactggtc gatgacttag
     4621 tcattcgctg tggtctggaa agcgccgttg aaatcgtcct ttttgccctt caactcaaat
     4681 ataaaccagt aaaaggcgca gtaaccacca tcattccacc taatcacgaa gcagaatcgc
     4741 tacctagtta gcatcaacgt ctatgtcatc atctttcact cgcttcagaa gatgagtggc
     4801 aacgctgtgt agataaagta ctcaccgcca ttccgacgct atcccccgca cggcaacctt
     4861 ttgcggcgtt actgctccct gaacgcccgg atatcgccca tgctatagat ctacgctatg
     4921 cagaccaaaa cgtcccggcg atgacctggt taagactggt gataaccgat gatactgctc
     4981 tgagtacagt ggaaaaatac gacttcccac cgttgtatcg tgattttcgt aattttcgcg
     5041 cttatctggc aatgttgttg gcaaataatg gggtgcgcgg tgtaagccgg atacttcttg
     5101 agtttacgga agaccattct gacaatccca cttatctctt cgaacgcatc agcgagactg
     5161 aaaatcttgt caaatggcta tggaaaacga atcacccgga tgcgattcaa attctgatcc
     5221 tcggtgtaat tggtaagaaa aagcacctgg aatacttaag caaggccagt caaaagcatc
     5281 ccgctgcggc tattgcggct tatgctactt tgctggcaat acatgaagat aaagagtggc
     5341 gtaaagcgct tgtcaaactg attaccgcca cgccagagtt agtatgcgac gtcattccct
     5401 gggttaatgc taaagcggca ggcatcttat ctgaatgtcg tccgcagtca gtcgctgaag
     5461 aatgtgaata tgccactgtg gatatgttgc cggagctgct tgtttcgccg ccctggatga
     5521 ccaaagagaa aaagaaaaat acgcctgtgt ttgatcttcc agtgcttcca gttccttctg
     5581 tttctgatgt cactccagag attaccaaaa aactgacccg tacttacctt gtcacccact
     5641 tccagcaaat agcccaacag caggcgacaa aacaaacatt gtttaccgac ctgccgccga
     5701 tcaaaaaagc aagctgggag aaacacttaa taccgcttac accggagcaa caaatactgt
     5761 ggcatcttgg ttttgaaaaa tggcgggaga gcggagaaaa aatatacgaa aaaattccgg
     5821 cgccacaaag cgccgttgat gccctgctac gcttcgattt tccagcactc aatgctgaat
     5881 ttgtgcatta tcataacaat gcatacaaaa gctggaatct cattgccctc tgttacctgc
     5941 ctggtcaaca ggccatttct tttcttaacc agatagtcaa agaagataac tactctggtg
     6001 agggaaatat tctggcaatt tttggtagcg ccgccatacc tgcatttatg gcctgcctgc
     6061 aacgtgatcc acgacgtctg tgcttcttcc cctttttcct cggtgtcagt gaactggcct
     6121 taccgatggc gcaacaatta cagaaaaaaa tgtcttatga agatgcgcga aactggctaa
     6181 ctgattatcc acgccatgcc gccgccgggc tgcttcctgt ggccctgggc aaaaaaggaa
     6241 aagatcgtga ttgtgcccgc caggcgcttc gtctgctggt gaatttaaac cagcgagaaa
     6301 cgattgagga aatcgcgcaa ggatataatc aacctgatgt tttagctgcg ctggcaacat
     6361 tgttcgatag cgacccactg gaagaatatc ccgctaaaat cgccccactg cccggctttt
     6421 atcagttcac cttgtggcgc agaccacggc ttaaaagtaa caacctgcct ctgtcagatg
     6481 acgctatgcg ccacctcggc actatgctga gctttcctcg cgacattacc gcctacgctg
     6541 ggctggatat cattagagaa atcttcaccc gcgaatcact ggctgaattt ggctgggatc
     6601 tgtatcccgc ctggacagaa gctggcgcac ccgcaaaaga aaactgggca tttacttcgc
     6661 ttgggatttt aggcaatgac gacaccgcgc gtaaattaac cccgcttatc cgcgcctggc
     6721 ctggggaatc ccagcataaa cgggcggtgt ccgggctgga tgtattagct gatattggca
     6781 gcgatgttgc gctaatgctg cttaatggca tcgcgcgaaa aattaaattc aaagcattac
     6841 aggaacatgc ccgcgagaaa atcaacatag ttgctgaaaa ccgtgggctg actatggctg
     6901 aactggaaga ccgcctggct ccagatttag ggcttgatag cagtggctcg ctgatactgg
     6961 atttcggccc ccgcaaattc accgttggtt ttgacgaaac cttaaaacct gtagtgtgcg
     7021 atgcaaacgg caaagtcctg aaagatttac ctaagccaaa ccagagcgat gaaaaaactc
     7081 aggcaactga cgcggttaat ctcttcaaac agttgaaaaa agatgtacgc gccatagcca
     7141 gccagcagat tgatcgtctg gaacaggcta tgtgccagcg cagacgctgg acggcagagc
     7201 agttccgcct gtttctggtg gagcatccgc tggtacgtca cttaacccga cggctgttat
     7261 ggggggttta taacgatgaa aacgccctta tcacctgttt tcgcgtggca gaagacagca
     7321 cttacagcga tgcgcaggat gagttattca cgctgccagc aggaaacatc ggtattccgc
     7381 atgtgctgga aatatcccct gaatccgccg ctgcattcag gcaaatttac gctgattacg
     7441 aactgcttcc ccctttccaa cagctcgagc gaggtagcta tcaccttgct gataatgaac
     7501 gtaatactca cgaactgaca cgctggcaag gacggctttg ccaggccgga cgcattgtcg
     7561 ggctggaacg cagaggctgg caacgtctgg aagaaagcgg cagcgtttat gcgatgcgca
     7621 aaacaacccc tcatggcgat ctcgaacttg aaacggaacc tttttcatta atttatggtg
     7681 aaacggggta tggcgaccag cacccggttg aaagcgtaaa aatcacctcg ccagatgatc
     7741 gctacggtaa acaatcctca ctcactttct ccatgctcga cgacatcacc gccagcgagc
     7801 tgattaacga cattgaatca ctgtttgatt aaaaggattt aacaatgaaa acctatattt
     7861 atcaggacga aaaatcacat aaattctggg cggtggagca gcagggaaac gagctgcata
     7921 ttaactgggg taaagtcggt accaacgggc aaagtcaggt aaaaagcttt gcagatactg
     7981 cggcggcgga aaaagcagca ctgaagctga ttgcggagaa ggtgaagaag gggtatgtgg
     8041 agcaagtaga cgctaacgct cactcacatg atgcctcagc aaagaaagtc ctggtcacag
     8101 aggaaaaagc ctcaataaat cgccaggcag caactaatgg tttaccctgg ctggcagaca
     8161 acgatcccat tattcttccc ccgaatattg cccgccatgc cctcagccac cgcctttggc
     8221 cgggagagcc agtaaaaaaa ccaaccaaac ccgagaaaca aaatttaatt tcccgattgg
     8281 caaatcgtac ttatcattat tataaaaatc tgactttcga ttacagctca tgctctgatg
     8341 aatgggctga tgcgattagc caggcaatca gcttaattaa ggcccaaaag actgagatac
     8401 ccgcgcgtac tatggcggta cttacttttc tggaaatatg tttttgtcag ggctggaatg
     8461 atgaaacctc atccgagttg ctggatgaaa ttgtaagcac cttcggtatc gaatacgcaa
     8521 ctgaaactgt catcttctgg tggcaaattg atttcgattt tgactataaa gatgaacatt
     8581 tgactttctt tattaattat gcagatagca gcaaggacag ttatagaagg aataacgaca
     8641 aatttagttt acgtctgcgt aaacatttat cactagcaga agaagaagta tggcaaaatt
     8701 gcattgcaaa attactcgtt gcgttaccag atatatcact ctatcgccaa ccgttaattg
     8761 ccattttaat gccagaaata cccgaagtgg cccatgagat cgtacatcgt ttacaccagg
     8821 tcgcagatgt tccacaactt gaaatgttga aactggtagc gacagatcca ttcacgctcg
     8881 aaatactgga gaactatcaa tacattgatg tatttaacac ctatggtgcc agttggtcag
     8941 ctacggtatt acgggagcaa ggtatagcgg cactggctcg cctgactccc tatgctgaag
     9001 aagataattg cggtgacgta ctgaaatgta ttaatcatcc gttggccatt acgaataaaa
     9061 agtgccgaaa attttccttc tgcaacgata gtggcattag ccgaagcatt ggtaaaaaaa
     9121 gatgataaac gcaaacatac attgctggtt tcattactta atgcaaatcc tttatttgtc
     9181 gaaaaattag tcatcggatt atcgtcgcaa gcggcaaatt tattgcaaca atgtcagcaa
     9241 ttgctgaata gtcgcaatga agattttgca aactcagaaa ttctaccaat ggtactagtc
     9301 tcgccgccct gggaaactca aaataaaaat aacgacaaca gtcctgtggg taaatacccg
     9361 gcgaaaatcc cccctttacc gttgttttat caacctccat tatggactcg cccgaaactc
     9421 aaaaatagca gcaaggtact gccagatagc gcactccagc acctcggtga aatgctccgc
     9481 ttccctcagg aagaggccct gtatccggga ttattgcagg tgaaagatgc ctgtaccgcc
     9541 gactcactgg ctgaatttgc ctgggatctg tttaccgcct ggctgactgc gggcgcaccg
     9601 tcgaaagaga gttgggcgtt cactgcgtta ggcgttcttg gtaacgatga caccgcccgc
     9661 aaactgacgc cattaatacg cgcctggcct ggtgaatccc agcataaacg cgccaccgtt
     9721 gggttggata ttctcgccgc tatcggtagt gatatcgccc tgatgcagct taacggtatt
     9781 gcccagaaac tgaaattcaa agcattacag gagcgggcaa aagaaaaaat tgccggcatt
     9841 gccgaaagcc gcgaactcac ggtggcagag cttgaagatc ggttagcacc ggatctcggt
     9901 ctggatgata acggttcgtt gctgctggat ttcggctcac gccagttcac cgtcagcttt
     9961 gatgaaacct taaaaccgtt tgtgcgtgat gtttctggca gccgtctgaa agacctgccc
    10021 aaaccaaaca aaagcgatga tgaatcacag gcgaacgatg ctgttaaccg ctacaaactg
    10081 ctgaaaaaag atgcgcgtac cgtcgccgcc cagcaggtag caaggttgga atccgccatg
    10141 tgcctgcgtc gccgctggtc gccagaaaac tttcagctct tcctggttga acatccactg
    10201 gtacgccact taacccgccg tctgatttgg ggcgtttata gcaccgaaaa ccagctactg
    10261 acgtgcttcc gcgtggcgga agataacagc tacagcaccg ctgacgatga tcttttcacc
    10321 ctgccggaag gcgatatctc tgttggcatt cctcacgttc tggaaatatc accgacggat
    10381 gctaccgcct ttggtcagct ttttgccgac tatgaactgc taccaccgtt ccgtcagctt
    10441 gaccgtaaca gttacgccct gacagaagcc gagcgcaacg ccagcgaact gacccgctgg
    10501 gcaggcagaa aatgcccgag cggtcgggtc atggggctgg cgaataaagg ctggataaag
    10561 ggcaccccgc aggatggagg ctggatcggc tggatgatca aacctctggg ccgctggtcg
    10621 ttaatcatgg aaatcgatga aggttttgcg gtaggcatgt cgccagccga actgagtgcc
    10681 gagcaaatct taagcaagct gtggctatgg gaaggcaacg cagaaagcta tggctggggg
    10741 agtaattcaa cacaggaagc gcagttctcc gtactcgatg ccatcaccgc cagcgagcta
    10801 attaacgata ttgaagccct gtttgaataa ggaaagagca tggacaagga attaccgtgg
    10861 ctggcggata acgcccaact ggaactgaaa tataaaaaag gcaaaacgcc gctcagtcat
    10921 cgtcgctggc cgggcgaacc agtgtccgtt atcactggaa gtctcatcca gacattggga
    10981 gatgaattac tacaacaagc aggccagaag gaaaatatca cctggaatta tgataaatgc
    11041 tcactggagt ggcagtccgc catccagcag gcaatcaatt tgactggcga gcataaaccg
    11101 tcaatcccag cccttaccat ggcggctctg atctgtatcg cgcaaaatga cagccagcag
    11161 ttacttgatg aaatcgtgca acaagagggg ctggaatatg cgacggatgt agtgatatcg
    11221 cgtcagtgca ttgcgcggcg ttatgagagt gactcactgg tcgtcacttt gcagtatcag
    11281 gacgaggact atggctacgg ttatggctcc gcgacctata acgatttcga tctccgtttg
    11341 cgtaagcatc tgtcgctggc agaggaaagt tgctggcagc gttgtgccga taaactcatt
    11401 gccgcactgc caggaatacc caaaattcgc cgtcctttta ttgcgctgat cctcccggaa
    11461 aaacctgaaa tagccaatga actcgccagt cttgaaagct cccggtcaag tctccattca
    11521 aaggagtggt taaaggtcgt tgctactgat aatacggcgg taaaaaaact cgaacgatac
    11581 tggggcctgg acgtatttag cgatcgcgaa gccagctaca tgtcacaaga aaaccgcttc
    11641 ggctacgcgg cctgcgcctc ccttttgcgc gaacaaggac tggcagccgt tccgcgcctg
    11701 gcaatgtatg ctcataaaga agattgcggc agtctgctgg tacaaattaa ccatccgcaa
    11761 gtcatccgca ccttgctgtt agttgctgat aaaaacaaac ccagcctgca acgtgtggct
    11821 aaatacagta aaaacttccc ccatgcgacg ctcgccgcac tggcagaact gctggcgtta
    11881 aaagaaccac cagcccgccc tggttatcca atcatcgaag acaaaaagct gcctgcacag
    11941 caaaaagctc gagaagaata ctggcgtacg ctgttacaaa cgctgatggc atcgcagcca
    12001 caactggcag cagaagtgat gtcgtggtta agtactcaag ccagggcagt gctgaagagt
    12061 tatttatcgg cactgccaaa accggttatt gatggcaccg ataacagcaa tctgcctgaa
    12121 atcctcgttt caccaccgtg gcgtagtaag aaaaaaatga cagctccacg tcttgatttg
    12181 gcacggctcg aattaactcc gcaagtttac tggcaaccag gcgaacgaga gaggcttgcc
    12241 gccactgagt ctgcccgtta tttcagcacg gaatctcttg cgcaacgcat ggaacaaaaa
    12301 agtgggcgag ttgtattaca ggaactgggt tttggggatg atgtatggct gtttttgaat
    12361 tatatactcc ctggaaaact ggatgctgca cgcaattcac tcattgttca gtggcattac
    12421 taccaggggc gggttgaaga gatcctgaat ggctggaact ccccggaagc acaattagca
    12481 gaacaggcgc tccgcagcgg tcacatagaa gcgttaatta acatatggga aaatgacaac
    12541 tactcacgtt atcgtccgga aaagagtgtc tggaacctgt atttattggc acagttgccg
    12601 cgtgagatgg ctttgacttt ctggctgcgt atcaatgaga aaaagcatct gttcgcggga
    12661 gaggactatt ttctcagtat cctcggattg gatgcgctac caggtctgct gttggctttt
    12721 tcacatcgtc caaaagaaac atttccgtta attttaaatt tcggcgcaac agaactggcc
    12781 ctgcccgttg cccgcgtctg gcgacgtttc gcggcgcagc gtgatctggc tcgccagtgg
    12841 attttacaat ggccggaaca tacggctact gcacttatcc ctcttgtctt caccaaatct
    12901 agcgataaaa gcgaagctgc attacttgcc ctgcgtttac tttacgaaca tgggcatggc
    12961 gaattgctac aaactgtagc caatcgttgg cagcgtacag atgtatggcc tgccctggag
    13021 catttactga aacagggtcc catggaaatt tacccggcac gcattccaaa agcccctgat
    13081 ttctggcatc cgcaaatgtg gtccaggccg cgccttatca ctaataatca acctgttacc
    13141 gatgacgctc tggaaattat cggcgaaatg ctgcgcttta cccagggggg acgtttttat
    13201 agcgggctgg aacaactgaa aacgttctgc cagccacaaa cgctggcagc ttttgcctgg
    13261 gatctcttca ctgcgtggca acaagctggt gcccccgcaa aagacaactg ggcatttctg
    13321 gcgttaagtc tctttggtga cgaaagcacg gcacgggatc taacaacaca gatcctcgcc
    13381 tggccacaag gcaaatctgc ccgtgctgtc agcggcctga acatccttac cctgatgaat
    13441 aatgatatgg cgctgataca gctgcatcat atatcgcaac gggctaaatc ccgcccctta
    13501 cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa atcgcgggct aagccaagaa
    13561 gagctagcgg acagattagt cccaaccctg ggccttgatg atccgcaggc gttgagtttt
    13621 gattttggtc cccggcagtt taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt
    13681 gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc gcgccgatga cgatcaactg
    13741 aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgctac tcaggtgagc
    13801 aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca cccgacgctg gtcgctggca
    13861 gattttcatt ctctgtttgt taatcatccc tttacccgtc tggttaccca gcgattaata
    13921 tggggggttt atccggcaaa tgaaccgcgt cgtttactca acgcctttcg tgtggccgca
    13981 gagggggggt tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga cgctctgatt
    14041 ggcattgccc acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt
    14101 gccgattacg aaattatgcc gccttttcgc cagttggcgc gccgcacggt gctgctcaca
    14161 cctgatgagt caaccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg
    14221 caacttatgg gaatgcgata caaaggctgg gagtcaggtt atgaggacgc atttgtctat
    14281 gacctgggtg agtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt
    14341 gatagcaaag cgttaatgag cttccgttct cttcgagtgt accgtgacaa taaatccgtc
    14401 acttttgccg aacttgatgt gtttaatttg agtgaggcgt taagcgcacc tgacgtcatt
    14461 ttccattaaa aacacagcgg gcagtgatgc aactgcccgt tatcaacacg acttaccagc
    14521 ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt tacgattatg aggtccctgt
    14581 acctgaagat ccgttttcct tcagacttga gatccataaa tgctctgaat tatttacagg
    14641 ttccgtctat cgactggagc gattccggct acgttcaaca tttcatcaac gtgatcgaga
    14701 agatgctgac ccgctaataa atgatgcgtt gatttatata agagatgagt gtattgatga
    14761 gcggaaatta cgaggtgaat cacctgaaac tgtaatagca atttttaatc gtgaactaca
    14821 gaatatattc aaccaagaaa tagaataata tactctagat aattaaagtc gcagcacatg
    14881 caacttgaag tatgacgagt atagctttct taaaggggga aacggtattc ccccttagat
    14941 gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc cactaataac
    15001 tccagtacgg caatgagcac ggttaacttt tgtgtcagcg tgtccatccg ctctccttat
    15061 taaggagcgc aacaccagca ccttaccctt acactgcctg tcgatactgg tttacgtatg
    15121 ttggtacgaa acgttgctct ccggatcagg gcaccgcctg cggcaccacc cgtccggcag
    15181 ccaggactta aaattggtcc gatccggcac aacctcccaa cgggcaggat tataaatctc
    15241 actcactgaa ttgcaatgga gtgtggatat ggttataatt tattcgttgg tacgaaatgt
    15301 tgcacaatca gcaagttagc gtcactgtag taaataaaag aacctgcctc accagcaggt
    15361 ttttttattt tctgcgatct actttccaga tatttttttg ccaatagccc accataaata
    15421 ttcacatccc ggtcaaatgg tgcgttgaat ataaaatcga tgaatttata aataaaaatg
    15481 agttatatga tgaattaata aaaacagtaa tcattttatt aaagggggaa gcggt
//
LOCUS       DAZFTN010000109        15348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFTN010000109 DAZFTN010000000
VERSION     DAZFTN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..181
                     /gene="hemP"
                     /locus_tag="ACHKV2_003847"
     CDS             <1..181
                     /gene="hemP"
                     /locus_tag="ACHKV2_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO6941646.1"
                     /translation="DSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDHDGQE
                     YLLRKTQAGKLLLTK"
     gene            complement(185..1621)
                     /gene="selO"
                     /locus_tag="ACHKV2_003848"
     CDS             complement(185..1621)
                     /gene="selO"
                     /locus_tag="ACHKV2_003848"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO6941647.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1684..2397)
                     /gene="ydiV"
                     /locus_tag="ACHKV2_003849"
     CDS             complement(1684..2397)
                     /gene="ydiV"
                     /locus_tag="ACHKV2_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO6941648.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(2644..3108)
                     /gene="nlpC"
                     /locus_tag="ACHKV2_003850"
     CDS             complement(2644..3108)
                     /gene="nlpC"
                     /locus_tag="ACHKV2_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6941649.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(3186..3935)
                     /gene="btuD"
                     /locus_tag="ACHKV2_003851"
     CDS             complement(3186..3935)
                     /gene="btuD"
                     /locus_tag="ACHKV2_003851"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO6941650.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(3935..4486)
                     /gene="btuE"
                     /locus_tag="ACHKV2_003852"
     CDS             complement(3935..4486)
                     /gene="btuE"
                     /locus_tag="ACHKV2_003852"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO6941651.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4549..5529)
                     /gene="btuC"
                     /locus_tag="ACHKV2_003853"
     CDS             complement(4549..5529)
                     /gene="btuC"
                     /locus_tag="ACHKV2_003853"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO6941652.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(5630..5929)
                     /gene="ihfA"
                     /locus_tag="ACHKV2_003854"
     CDS             complement(5630..5929)
                     /gene="ihfA"
                     /locus_tag="ACHKV2_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO6941653.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(5934..8321)
                     /gene="pheT"
                     /locus_tag="ACHKV2_003855"
     CDS             complement(5934..8321)
                     /gene="pheT"
                     /locus_tag="ACHKV2_003855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO6941654.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(8336..9319)
                     /gene="pheS"
                     /locus_tag="ACHKV2_003856"
     CDS             complement(8336..9319)
                     /gene="pheS"
                     /locus_tag="ACHKV2_003856"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO6941655.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(9528..9652)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(9602..9646)
                     /gene="pheM"
                     /locus_tag="ACHKV2_003857"
     CDS             complement(9602..9646)
                     /gene="pheM"
                     /locus_tag="ACHKV2_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO6941656.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(9769..10125)
                     /gene="rplT"
                     /locus_tag="ACHKV2_003858"
     CDS             complement(9769..10125)
                     /gene="rplT"
                     /locus_tag="ACHKV2_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO6941657.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(10178..10375)
                     /gene="rpmI"
                     /locus_tag="ACHKV2_003859"
     CDS             complement(10178..10375)
                     /gene="rpmI"
                     /locus_tag="ACHKV2_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO6941658.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(10472..11014)
                     /gene="infC"
                     /locus_tag="ACHKV2_003860"
     CDS             complement(10472..11014)
                     /gene="infC"
                     /locus_tag="ACHKV2_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO6941659.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(11018..12946)
                     /gene="thrS"
                     /locus_tag="ACHKV2_003861"
     CDS             complement(11018..12946)
                     /gene="thrS"
                     /locus_tag="ACHKV2_003861"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO6941660.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            13469..>15348
                     /gene="espL1"
                     /locus_tag="ACHKV2_003862"
     CDS             13469..>15348
                     /gene="espL1"
                     /locus_tag="ACHKV2_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO6941661.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     AD"
BASE COUNT         3936 a         4013 c         3495 g         3904 t
ORIGIN      
        1 ggatagcaga aaactcacgc ctgaaacgga tgccaatcac aagaccgctt ccccgcagcc
       61 tattcggcga atttccagcc agacactgtt aggtccggat ggcaaactga ttatcgatca
      121 tgacgggcaa gaatatctgc tccgtaaaac tcaggctggc aagctgctgt tgaccaagta
      181 gcctttaact cgagcagctg acttccagcc gtttacccca gtcaggtgga cgactgacat
      241 agtcatcatc tctgtcgctg aaaggatttc gcaacgcctc atgcaggcgg tgcaattccg
      301 tcatatcacc cttttctgcc gcttcaatcg cccgttgcgc caaccagttg cgcaatacca
      361 gagcggggtt aacgctttgc atcagttgct gacgctcact atcgctaacc tcgtcttgtt
      421 gcaaacgccc tcgataacgg gcaaaccagt catcaaatgc cgcacgatca ataaactcat
      481 cacgtagcgg tgacgccgcg ctgtgctgct cggtcagact cagcatgcga aatgtgcggg
      541 tataatcgct gcgctctcgc gccatcagac tgaataattc attcagtagc gcgttatcct
      601 ctttttgctc cgtcataaag cccagtttct gccgcatccg ttgtccataa tgcgtcaaca
      661 aaacctgctg atagctgtcc agggcctcat tcagggcatc tacggcaaca aatggtgaca
      721 atgtctgcgc cagacgctgt aaaatccaca acgcgacagc aggttgattg tcaaagctgt
      781 aacgtccttg atgatccgag tgattacaaa taaaaccggg ttcgtaatca ttaagaaaac
      841 caaacggccc gtaatcaagc gtcagtccca gcagcgacat gttgtcggta ttcatcaccc
      901 catgtgcaaa gccgaccgtc tgccattggg caattaacga tgcggtacgt gcgacaacat
      961 cgttaaacca gagacggtat ttgtcctcat catcttcaag atgtgaccag taatgacgaa
     1021 tggcaaagtc agccaactga cgaaccttat ccggctcgcg gcgatagtaa aaatgttcga
     1081 aatgaccaaa gcgcagatga cttggtgcca cacgcatcag catcgcgcct ggctccaccg
     1141 tttcccgata cactggcgaa tcgctggtga cgatacttaa cgcgcgggtc gtcggaatgc
     1201 ccagataatg catcgcctca ctggcgagac tttctcgtat cgtcgaacgt aataccgccc
     1261 gtccatcacc cattcgcgaa taaggcgtca ggccagcacc tttcagatgc cagtccattg
     1321 tagtgccatc agcaagcagt tgttcgccaa gtaaaatgcc gcgcccatca cccagttggc
     1381 ccgcccagac gccgaactga tgaccactgt aaacctgcgc cagtggtgac ataccaggca
     1441 gtagggtttc gccgccccag acacctgcgc catttttaaa cagcgacgat ggaatactca
     1501 gcgtgttagc gagttcggta ttatgccaaa tcagccgggc attatttaaa ggcgtagggg
     1561 aaagtgctgt ataggtttct ggcaattcat cgcgccagcg ggtaataaaa gacagggtca
     1621 tagagcctcc tgtttgatag tgtagacggt tactctcgtc ttaaacacca gcaaacagga
     1681 gggttatcgc tgaaccaacg tcgttatctg atttatggga acggcaggcc acaggcatcc
     1741 ctggagagca tggaaatcga agggggtaat ctgtgctaat atttctggcg tatcaatgcc
     1801 ccccgcgatg atgcaattgc aacaagggga aatttgcgcc tgaatcgcac gaataaacgg
     1861 ttcgaaggag cgatgcgtga tttgttgctg aataaaactt ttatccagca ttacgcgggt
     1921 gaaaagacca tcaaaaaccg ctttcattgt gctgttgcct gctcccaaat tacctaacac
     1981 aaggggatac acttgcgata aggataaaag accgcgatta tcttttcctt cattgagatg
     2041 cgggtaattc tcattaatca acagttcaat aaacggatat tttaatagtt cgcctgcgta
     2101 attatccctc tctaataata atgtagccac ttgtggcgtt aaattaagcc aggcaaagag
     2161 tttatgttga ataaaaaaat gttggcatga ttttagtaat tccaactgct ctgaaaataa
     2221 ttgccaatgt tgctcctcgg taagttgcgc tataacccga ctcgtcggga tacgcaccgt
     2281 gccatcttca ctggagaaat gggtaattaa ctcaacgccg acaaggactt gctggttatc
     2341 tcttatcggc aggaaatagc aatcagaatg ataaagattc tccaaaaaaa tcttcatggt
     2401 aaccgtccct ctcgaaggat gtttttcagt atccggctaa aagggatgag gccataagac
     2461 ataacaatta cagaaggagt aactttcatt tgttccatgt taaccacttt ttcaggggtc
     2521 ctttttttag attatcctga ttataaacga ataatccatt tacggaattt ttgtctgcaa
     2581 aatactactg tattctggag taaatggacg gcgatgtatc atcgacgtcc aattttcgac
     2641 atactagatt cgtctcgcct gccagaaatt tttctgccaa tagacattat caagtgagga
     2701 acgcatcact cccttgctgg tagaggcgtg gataaattgg ttgttggtat cgtaaatacc
     2761 tacatgcaaa ccattttgtc cggaacccgt tttgaaaaag accaggtcac caggcagcaa
     2821 ctcgtcttta tcaatttgcg tgccgataga ggcttgttct ttggtttctc ggggcagctg
     2881 caaatcgaaa cgatcgcgca tcgtcacaac cacaaatccc gaacagtcca caccgcgccg
     2941 cgtcatgcca ccataacgat acggcgtgcc atgccagctt tgtagctggt cgttcaaacc
     3001 ggcaataacg gtaatcgaat cagaaagtct ggcatttggc ggcggtgctt tatggtggct
     3061 acacccggcc agaaacagtg ctgtgatcaa aataaggcag aaacgcattc cgtacggttc
     3121 ctctgttttt tattcttgca ttaatttagc gtcgtaatta cccgattttc aagatactaa
     3181 tgaaatcaga tggtcgaaat cagcattctg tgaccttcga tatccagacg gcgaaaattc
     3241 atcccatagg cctgcgccag atttggcggc gtgagcacat cttctctgcg tccactggcc
     3301 agcatttttc caccttttag caaccacgcc cgatgcgcat gacgcaatgt gtggttgaga
     3361 tcgtgactgc tcatcacaat cgccagtcct tgctgacaca gcgcgctcag aattttgtct
     3421 aacgcacttt gttgcgcaac atcaagactg ttcatcggct catcaagaag cagcaattgg
     3481 cctgcgggat tggcttgtgg tgtgatttgc agcaccaccg cagcaagacg tacgcgttgc
     3541 cattcaccgc cggaaagttg attggtgcta cgaccgagtt tgtcatcaag agccagcgcc
     3601 cctgcgacat cattcagtag ttcggtacgc gttttatcgt gctgatgcag tgtcaggtag
     3661 tgccagaccg gcattgcaaa cggcggcgtc tgctgttgtg aaagataggc acgatgcagc
     3721 gcgagttttg ttgcggacca tgcttccagt ggttgccccg cgaactgaat gcttccctta
     3781 ccgctggtca ttccggccat tcgcgccagt aaggtactct tacccgcgcc attcggcccc
     3841 accaggtgca ggatctcccc agcccgaacc tcgccagaaa gcggccccag gcgggtagat
     3901 tccgcaacat cctgtaactg catcacaata gacattattt tgccaacgcc agtttaatgc
     3961 tttccatcac aatgggatct tccggcgtca tatccgggga aaaacgctgg atgaccaatc
     4021 cgtccctgcc aactaaaaat ttctcaaaat tccataaaat atcatcaggg tacagcggtg
     4081 cacgaccttt gctgaccata cgggcataga atccgctctc ttccggcgcg actgcggtcg
     4141 gcgctgcgga aatcaatttt tgatacagcg gatggcgtcc ttcgccatta acttcaatct
     4201 tactgaacat cgggaacgtc accccccatg tggtggtaca gtaagtttta atctcttcat
     4261 cgctgcccgg ttcttgttcc agaaactggt tgcacgggaa tcccagcacc acaaaacctc
     4321 gatcggccca ggctttctga atattctcca actgctcata ttgcggcgtt aagccacact
     4381 ttgaggcgac attgacaatc aacagcacat taccggcgta cttctccagc gtggtcactt
     4441 caccgtcgat atctttcact acggtcgtca gaatggaatc ttgcatcgtt tctcctgggt
     4501 gtggtcagta aaaatcttag cttttaatca tagaccgtct ttttgcggct aacgtcctgc
     4561 ttttaacaat aaccagataa acaccggcgc acccaacgtt gcggtgacca cgccaatagg
     4621 cagctctgcg gcagctaatg ccaggcgcgc tacaatatcg gccagcagca atgcgctcgc
     4681 ccctgccagc gcgcagccgg gaagtaatgc gcgatgatcg gttaaaccac acaaccgaag
     4741 aatatgtggg atcaccagac caataaagcc gatagcaccc gccagcgcta cactgacgcc
     4801 aaccatccag ccggtcgctg ccaccagcac attgcgccag aaccacaggg gtaaacccag
     4861 ttgccgcgcc gagatctcgc caagtgctaa catattcatc ggcctggact gacaacagat
     4921 ccacaacaac atggggatca atgccagcat cagccagctt tgccgccagt ctacgccgcc
     4981 aaaaccgccc atcatccagt acatcagctg acgtaaatca acagaggtgg aaaagtagat
     5041 agcccacgtc attagtgcgc tacagataat ccctaatgca acgccagcca gcaataaccg
     5101 actggtcgaa agatgacgac gggcgaaacg taagagtatt aaagtgatga taagcgcccc
     5161 agcaatcgca cacagcccta gcgcccagtt ggggagttgc ccttgcccaa gcaataccgc
     5221 ggcgataagc cccacgcctg cgccgttaga gacgccaagt agtccaggtt ctgccagagg
     5281 attttcaaac aacgcctgca ttacagcgcc ggatatagcc agcgccgcac caaccagcaa
     5341 tacagccagc gtacgtggca ggcgaatttg ccagacgaac agttcgccac gaggagaaaa
     5401 ccagtcacct ggcgagatcc attgttcacc ggcgcaaagg cttaagagaa gcgccagcag
     5461 catcaaaact gacaggcata ataaccagcg aatattttgt cgctgttgtt ggcgggcaag
     5521 tgtcagcatg gtatccgttc tgctgaagtg tcatggcgtt gattttacgg tgactcttcg
     5581 acagtgaaaa gaaaaaaggc cgcagagcgg cctttttagt tagatcagat tactcgtctt
     5641 tgggcgaagc gttttcgacc cggcttttta acttctgccc gggtctgaag gtcaccacgc
     5701 gccgtgctgt aatgggaata tcctcgcccg ttttcgggtt acgtcccggg cgttgattct
     5761 tatcacgcag atcgaagtta ccaaaaccag agagtttcac ctgttcgccg ttttccagag
     5821 cgcgacggat ctcttcgaaa aacagttcaa ccagttcttt ggcatcccgc ttgctaagcc
     5881 caagcttatc aaacagatat tctgacattt cagcttttgt aagcgccata ggttcaatcc
     5941 ctcaatgatg cctggaatcg ctcttttaat gcctctacac atttggcgac ggtagcggca
     6001 atctcctctt cttcgagtgt acggctggta tcttgcagga tcaggcttat ggcgaggctc
     6061 ttatacccct ccgcaacacc cttaccgcgg tacacgtcaa ataagtttac gccaactacc
     6121 tgatttacgc caactttctt acattcggat aaaatatccg ctgcgggaac gttttctgcg
     6181 accaccaccg cgatgtcacg acggttcgcc gggaagcgag aaatctcgcg cgcctgaggc
     6241 accacgcggt ctgcgagctt gttccactcc agttcgaaca ccagagtgcg accgttaaga
     6301 tccagtttac gttccagttc aggatgaaca accccaacaa aaccaatacg ttcacctttc
     6361 agataaatcg ctgcggattg ccccggatgc agtgccggat tcgcttctgc acggaactca
     6421 acctcattca gtttaccggt caggtcgaga acggattcaa gatcgccttt caaatcatag
     6481 aaatcaacgg tctcttttgc caggttccag tgctcttcgt aacggttacc gcaaatcaca
     6541 ccggctaaca tcagatcctg acgaatgccc aacggtgcct gagtatctgg tacgaaacgc
     6601 agaccgcttt cgaaaatgcg cacacggttc tgctgacggt tctggttgta caccacggtt
     6661 gccagcaggc cggtccacag agaaagacgc attgctgaca tttcaacaga gatcgggctt
     6721 ggcagcagta aggcttcaac gcctggatgg atcatctgct gcactttcgg atcaacgaag
     6781 ctataggtga tcacttcctg atagccttta tcgttgagca gcgttttcac gcgcttgagc
     6841 gacaggtcag cttcacggtg agtacccata atcaggcttg cctgtaccgg ctcatccggg
     6901 atgttgttgt agccgtaaac acgcgcgact tcttcgacca gatcttcttc aatctccatg
     6961 tcgaaacgcc agctcggcgc aactgcctgc cactcgtctt tgccttcggt cacttcgcag
     7021 ccgagacgac gcagaatgtc agttacctgc tcatccgcaa tatgatggcc gatcaggcga
     7081 tccagtttgc tacgacgtaa agtgatggtt gcacgcttcg gcagcgttgc ttcgttggtg
     7141 atatcaatta ccggaccagc ctcaccaccg cagatgtcaa tcagcagacg ggtcgcacgt
     7201 tccatcgctt tgtactgcag tgccggatca acgccacgct cataacggtg agacgcatca
     7261 gtatgcaggc catgacgacg agcacgaccg gtgatagaca gcgggctaaa gaaagcgcat
     7321 tccagcagca cgttttgtgt ttcgtcattc acgccagagt gttcgccacc gaagatgccg
     7381 cccatcgcca gcgccttgtt gtggtcggcg atgaccagag tgtcagcatt cagcttcgct
     7441 tcagtaccgt cgagcagcac cagcgtttcg ccctctttcg ccatccgcac cacaatgccg
     7501 ccttcaatgc gatctttatc gaaagcgtgc atcggctggc ccaattcgag cagcacatag
     7561 ttggtgacgt caacaactgc atcgatagaa cggatcccgc aacgacgcag tttttctttc
     7621 atccacagcg gagttggcgc tttaacgtta atgcctttta ccacacggcc aagataacgc
     7681 gggcaggctt ccggcgcttc gactgtaatc ggcagcgtgt cgtcgatggt cgcaccaacc
     7741 ggaacgattt ccggttcaac cagcggcagc tgattcagca cggcaacgtc acgcgcaaca
     7801 ccaatgatac ctaaacagtc ggcacggttt ggcgttacgc tgatttcgat ggtgttgtca
     7861 tcgagtttca ggtattcgcg gatgtcagtg ccaatcggcg catctgcagg cagttcgata
     7921 atgccgttat ggtcgtcaga aattcccagc tcagaaaagg agcacagcat cccttcagac
     7981 ggttcgccgc gcagtttcgc cgctttaatt ttgaaatcac ccggcagaac agcaccaatg
     8041 gtcgctaccg ccacacgcag gccctgacgg cagtttggcg caccgcagac gatgtccagc
     8101 aggcgatcgc cgccgacatt cacttttgtc acacgcagtt tgtcagcgtt cggatgctgc
     8161 gcacactcaa ccacttcacc aacgaccacg ccgtggaagc taccggcaac cggttctaca
     8221 ccgtcaactt ccaggcccgc catagtgatt tgattcgcca gcgcatcgct atcaatcgcc
     8281 gggttcaccc attcgcgtaa ccacagttca ctgaatttca taatctattc ctgccttatt
     8341 taaactgttt gaggaaacgc agatcgtttt cgaagaatga acgcaggtcg gtgacgccgt
     8401 aacgcaacat agtcagacgc tccatcccca tcccgaaggc gaaaccagag taaacttccg
     8461 ggtcgatgcc aacgttacgc agcacgttcg gatgcaccat cccgcagccc agtacttcca
     8521 gccatttacc gtttttaccc atgacatcca cttctgcaga aggttcggta aacgggaagt
     8581 aggaaggacg gaagcgaatc tgcaaatctt cctcaaagaa gttacgcagg aagtcgtgca
     8641 gcgtgccttt caggttggta aagctgatgt tggtatcaac aatcagacct tccatctgat
     8701 ggaacatcgg cgtgtgagtc tggtcgtagt cgttacgata aacacggcca ggcgcgatga
     8761 tacgaatcgg tggctgctgg gctttcatgg tgcggatctg tacgccagag gtctgggtac
     8821 gcagcaggcg ggtagcgtca aaccagaaag tgtcgtggtc agcgcgcgcc gggtggtgac
     8881 caggaatgtt cagagcatcg aagttatgat aatcgtcttc gatttccggc ccggttgcca
     8941 cggtaaagcc aagctcaccg aagaaacttt cgatacggtc gatggtacgg gtaaccggat
     9001 gcagaccgcc gttttcaatg cgacgacctg gcagagagac atcaatcgtt tccgccgcca
     9061 gacgcgcatt cagtgcagcg ctttccagtt ccgctttacg cgcattcagc gcctgctgaa
     9121 cctgctcttt cgcttcgttg ataaccgcac cggctgccgg acgctcttct ggcggcagct
     9181 cacgcagggt cgtcatctga agggttaagt gccctttttt acccaaatat tcgacgcgca
     9241 cattgtctaa cgcggcaaca tctgacgcct ggctaatggc cgccttcgca ctggcaacca
     9301 gttctgcgag atgtgacatg gttttcctca ttgtgtcagt ggtgacactg gttcgttgga
     9361 cttagagcct atcccatcag gctattttac ttgccatttt ggtcccgggc agtgctcgaa
     9421 atcctcacgt acttaagtac gctccggttt ctccgcactg gccgtatcca gactggctgc
     9481 gacaattacg cctgatagac tggcttttta tttttcaaaa cgcgcataca aaaaaagcct
     9541 ccactgggag gctttcaggc gctgttttcc gtttctcttc tcacgcgcta gcctcctgga
     9601 ttcaggtgct aaagtaaaaa aagaagcgga aaatagcagc attcattgct tgcgttacct
     9661 tttggtactc ttcaaaagac ctttattgaa aaggctacgg cgataaaagt caatgttttg
     9721 atggcgttga aacgaaaaga gggagactag ctccctcttt caactggctt atgccagagc
     9781 tgctttcgct ttttcaacca gagcggtgaa cgctactttg tcgaatactg cgatatcagc
     9841 caggatctta cggtcgattt caacagaggc ttttttcagg ccattgatga atttgctgta
     9901 agaaataccg ttctgacgtg ctgctgcgtt gatacgcgca atccacagtt gacggaactg
     9961 acgcttacgt tgacgacggt cacggtaagc atactgacca gctttgataa cagcctggaa
    10021 ggcaacgcgg tatacgcgag aacgcgcacc gtagtagcct ttagcttgtt tcaaaatttt
    10081 cttgtgacgt gcgcgtgcaa taacaccacg ttttacgcga gccatatgtg ctctcctgta
    10141 tctatattct aattaaaaag ttaaaaacgt taacggctta tgcgtacggc aggcacgcga
    10201 ttaccaggcc cagatcgcct ttggaaacca tggctttcgg acgcaggtga cgtttacgtt
    10261 tggtcgcttt tttggtcaga atgtgacgca ggttagcgtg cttgtgctta aaaccacctt
    10321 taccggtttt tttgaagcgc ttagcagcac cgcgtacggt cttaattttt ggcattttaa
    10381 taacttccac ttcgcattgt taataaacga aacaaaggcg aacaaagcct gtgaagcccg
    10441 aaggctccac agacagtgct acttgaaggc cttactgttt cttcttagga gcgagcacca
    10501 tgatcatctg gcggccttcg atcttcgttg ggaaggattc gaccactgcc agttcttgca
    10561 aatcgtcttt cacgcgatta agcacttcca taccgatttg ctggtgcgcc atctcacgac
    10621 cgcggaaacg cagcgtgatt ttggctttat caccctcttc gagaaagcga atcaggctgc
    10681 ggagttttac ctgatagtcg ccttcatctg taccaggacg gaatttaatt tccttaacct
    10741 ggataacttt ttgctttttc ttctgttcct tagaagactt gctcttttca tagaggaatt
    10801 tgccgtaatc cattatacga caaaccggcg gctcggcgtt agggctgatc tcgactaagt
    10861 ctactccggc ttcttctgct ttctccagag cttctctcag actcacaata ccaagctgct
    10921 cgccttccag acctgttaag cgaacttcct gggcgcgaat ttcgccattg atacggttag
    10981 ggcgcgccgt ttgaactcgt tttccgcctt taatacctta ttcctccaat tgtttaagac
    11041 tgcggctgcg aatctcttgt tgcagcttct cgatcacttc atttacgtcc atgcttccca
    11101 ggtctttacc acggcgggtg cgaacggcaa ttttgcctga ttccacctct ttatcaccac
    11161 agaccagcat atacgggacg cgacgcaaag tgtgctcgcg gattttaaag ccaatcttct
    11221 catttctcaa gtctgcttta acacgaatgc ccgcatttga tagtttttgc gtcaattcgt
    11281 taacgtattc agactgtgaa tcggtaatat tcatgataac aacctgaact ggcgcaagcc
    11341 aggtcgggaa gaaaccagcg aattcttcgg tcaggatacc gatgaaacgt tccatcgacc
    11401 ccagaattgc gcggtgaatc attaccggta ccttacgctc gttgtcttcg cccacatagg
    11461 aggcgctcag acgagacggc aaggagaagt ccagctgtac tgtaccgcac tgccatgcac
    11521 gatcgaggca gtcatacagg gtaaattcaa ttttcggacc gtagaaagcg ccttcaccca
    11581 gttgatattc aaacgggatg ttgttttctt ccagcgcaac cgccaggtcc gcctcagcac
    11641 gatcccacat ttcgtcgctg ccaatacgct tttcaggacg agtggagagt ttgacgacga
    11701 tcttctcgaa gccaaaagtg ctgtacatat catagactaa acggatacat ccgttaactt
    11761 catcgcgaat ttgttcttca gtacagaaga tatgcgcgtc atcctgggtg aatccacgca
    11821 cgcgcatcag gccatgcaga gaacctgacg gctcgttacg gtggcagcta ccaaactcgg
    11881 ccatacgcag cggcagatcg cgataagact tcagcccctg gttgaaaatt tgtacgtgac
    11941 ccgggcagtt catcggctta atgcagtatt cacggttctc agaagacgtg gtgaacattg
    12001 catctttgta gttgtcccag tgaccggttt tttcccacag gacacggtcc atcatgaacg
    12061 gacctttaac ttcctgatac tggtactctt tcagtttaga acgaacaaac acttccagtt
    12121 cacggaagat ggtccagccg tcgttgtgcc agaataccat acccggcgct tcttcctgca
    12181 tatggtacag gtcgagctgt ttaccgattt tacggtggtc gcgtttcgcg gcttcttcca
    12241 ggcgctgcag gtaagcgtta agcgcttttt tgtctgccca cgccgtaccg taaatacgtt
    12301 gcaacatttt gttgttgctg tcgccacgcc agtaagcccc tgccgttttc attagtttga
    12361 aatgatggca gaaacgcatg ttcggtacgt gcggaccgcg gcacatatcg acatattctt
    12421 catggaagta cagacctggc ttgtcatcat gggcgatgtt ttcgtcaaga atggagactt
    12481 tgtagctctc cccacggttg gcgaaagttt cacgcgcttc gtgccagctg actttcttct
    12541 taatgacgtc gtagtttttc tcagcaagct catgcatccg cttctcgagt gcttcgacat
    12601 cttcctgggt taacgtgcgg tcaagatcaa cgtcgtaata aaaaccgttg tcaataaccg
    12661 ggccgattgc cattttggta tgcggccaaa gttgtttaat cgcgtgccct aacaggtgcg
    12721 cacaggagtg acgaatgatc tccagacctt cttcgtcttt ggcggtaatg atcgacagtt
    12781 gtgcgtcgtt ttcaatcaga tcgcaagcat caaccagttc gccattaacg cgccctgcga
    12841 tacaggcttt cgccagacct ggaccaatgt ccagcgcaac atccatgggg cttacagcgt
    12901 ggtcgtaatg gcgttgactg ccatcaggaa gagttataac aggcatttta tatccttatt
    12961 tgcagtggtg acccacacga aagatcacat acaaagaaaa atttgtttat taacagttca
    13021 ttgcgaaacc atctagccaa caaatgctaa attggttcgc aatcaggtac acaaatgaaa
    13081 gacattaacc tctcaccttc cacccggttg agcgatgtta acactaaaaa aagggagatt
    13141 gtacctttcc gtttcacatt gattcgattc gcaattcgtt tgtagtgagt acaccagcaa
    13201 aacgcttaaa gaacaactga taacatcacg gagcgtctga aaggtttact gcgagcgaat
    13261 ttacagcagc ataaactgaa tccagcaaag ttcgttgcgt gatttggcgg tactatttaa
    13321 acgatcataa gattaatgac gaagtggtca tatcacaatg ataaaagtga cacaattctt
    13381 atagcaattt ttcgtgcaca tttcgttctg gcgataataa ttaatcatca tactcaaaac
    13441 ctccagacat atataaggcg aatagattat gagtcaaaac gacattatta tcagaactca
    13501 ttataagtct cctcatagaa tgcacatcga tagcgacata ccaacgcctt catcagagcc
    13561 tattaatcaa tttgcgcccc aactcatcac cctacttgat acctctgact taagttcgat
    13621 gctgtcatac tgtgttactc aggaatttac cgcaaactgt cgaaaaatat cacaaaattg
    13681 ttattccact gcccttttta ccattaactt tgccacttca cccatccatg cagaaaatat
    13741 attcattaca ttacactata aaaaagaaat catttcctta ttactggaaa ccacgcctat
    13801 taaagctaac catttgcgaa gcatactgga ttatattgaa caggaacagt taactgccga
    13861 aaatcgtaac cattgtatga aactgtctaa aaaaatccat agagaaaaaa ctatacaacc
    13921 aacagtaaat ctcaatggta gtgcattttt ttcgcaatct ccttctgacg ctattttttg
    13981 tcgccatctg tcattgcaat acgcacttga ttcattgaga aatggaaaag gcaaggtcaa
    14041 cctgattaaa cattactcct ccgttgaatc catacagcag catgtcccct tagtccggga
    14101 cgcggagttc agagcattac ttcgccatcc tcctgcaggg agtcgcgtta tcgcgagtaa
    14161 ggattttggc ttcgctttag atattttctt ctgtcgaatg atggcaaaca atgtcagtca
    14221 tatgtctgcg attttatata tagacaatca tactttgtca gtaaggctac gaataaagca
    14281 gtcggcgtat gggcaattaa attatgttgt gtccgtttac gacccgaacg ataccaacgt
    14341 tgccgtcaga ggcacccaca ggacagcacg gggctttctc tcgcttgata agttcatcag
    14401 ttcaggtccc gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat
    14461 tctgccccta ttacctgagg gagttccagg ggctattttc acgggtatcg cgacacgaat
    14521 gccatttgcc cctatacatc catcggcaat gttgttaata atggccacag gccagactca
    14581 acagcttatt acattattca gacagttgcc catactccct gaaaaagaaa tcattgaaat
    14641 aataactgcg cagaatagca ttggtacacc tgctttattt ttggctatga tgaacggaca
    14701 tactgacaac gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat
    14761 tcatgaagat aatctggtta aattgttgca aactaaaagt gctaacgaaa cacctggact
    14821 ttatatctcc atgttgtatg gattcgatga gataatcgat atctttctga atgcattaac
    14881 cactcctata gcacaagagc ttttaaacaa aaaactggtg atgagtattt tagccatgaa
    14941 aatacatgat ggtgagccag gattatacgc cgcaatggaa aataatcacc ctttgtgtgt
    15001 cacacggttc ctctctaaaa ttaatggcat cgcctttaaa tacaagttga gcaaagctaa
    15061 catcatggat ttattaaaag gcgctacagc acagggaacc cctgcattat acatcgctat
    15121 gagcaagggt aatgaagacg tcgtgttatc ttatatatcg acgctgggtg cttttgcaaa
    15181 aaaacattct tttagtcaac atcagttatt tacactgttg gctgctaaaa atcatgacaa
    15241 catgtcagct gttcatatag ccattcatca taatcattat aaaactgtag aaacatatta
    15301 tgctgctata aatgtaatca gccaaagcat gagttttagt gctgatga
//
LOCUS       DAZFTN010000110        15070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFTN010000110 DAZFTN010000000
VERSION     DAZFTN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            137..349
                     /locus_tag="ACHKV2_003863"
     CDS             137..349
                     /locus_tag="ACHKV2_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO6941662.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            361..642
                     /locus_tag="ACHKV2_003864"
     CDS             361..642
                     /locus_tag="ACHKV2_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO6941663.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVVLSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            663..944
                     /gene="gamL"
                     /locus_tag="ACHKV2_003865"
     CDS             663..944
                     /gene="gamL"
                     /locus_tag="ACHKV2_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFO6941664.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            961..1911
                     /locus_tag="ACHKV2_003866"
     CDS             961..1911
                     /locus_tag="ACHKV2_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO6941665.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQG
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            1908..2597
                     /locus_tag="ACHKV2_003867"
     CDS             1908..2597
                     /locus_tag="ACHKV2_003867"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6941666.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            2597..3184
                     /locus_tag="ACHKV2_003868"
     CDS             2597..3184
                     /locus_tag="ACHKV2_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO6941667.1"
                     /translation="MDEVIFTYNEESALTAGQGSFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNVNPNHTDDPGYWQYGSDSF"
     gene            3259..3606
                     /locus_tag="ACHKV2_003869"
     CDS             3259..3606
                     /locus_tag="ACHKV2_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941668.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            3670..4491
                     /locus_tag="ACHKV2_003870"
     CDS             3670..4491
                     /locus_tag="ACHKV2_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO6941669.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKKQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            4568..4963
                     /locus_tag="ACHKV2_003871"
     CDS             4568..4963
                     /locus_tag="ACHKV2_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941670.1"
                     /translation="MQFKDLPADIQKIAADTLKAHLSVLNLIKEPKTNLENISRNVRD
                     VFVGLYAYDNEKHEEHIQNGCINKCQQHIKLPVADKTEQKTELLVFWNTVEAIARSKM
                     FEFNNINHSGIDTHQSSHREGVSMLLALL"
     gene            5114..5839
                     /locus_tag="ACHKV2_003872"
     CDS             5114..5839
                     /locus_tag="ACHKV2_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6941671.1"
                     /translation="MTTEIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGI
                     DIDAIHAFKIMAGPETVLSLLDERDALNERIAELEANLAKLAEDQQKAIESIKQADSA
                     VKLAHEKFSALAAENAGLNKFIAQSCYVFDGEQDELSDAYICATDGGMPQIPATDAFL
                     AEIRAEARNEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANESHPIDG
                     LSGEYAEKSLEEWAERLRKGGNQ"
     gene            5836..6243
                     /locus_tag="ACHKV2_003873"
     CDS             5836..6243
                     /locus_tag="ACHKV2_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6941672.1"
                     /translation="MSKIDYQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            6245..6436
                     /locus_tag="ACHKV2_003874"
     CDS             6245..6436
                     /locus_tag="ACHKV2_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941673.1"
                     /translation="MNGQISIVRPGACDDSEIHMIIRLARGKTITVLTTPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            6439..6888
                     /locus_tag="ACHKV2_003875"
                     /pseudo
     CDS             6439..6888
                     /locus_tag="ACHKV2_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            7054..7335
                     /locus_tag="ACHKV2_003876"
                     /pseudo
     CDS             7054..7335
                     /locus_tag="ACHKV2_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            7691..8329
                     /locus_tag="ACHKV2_003877"
     CDS             7691..8329
                     /locus_tag="ACHKV2_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO6941674.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFIESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMR
                     ILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            8385..8816
                     /locus_tag="ACHKV2_003878"
     CDS             8385..8816
                     /locus_tag="ACHKV2_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO6941675.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            8813..9439
                     /locus_tag="ACHKV2_003879"
     CDS             8813..9439
                     /locus_tag="ACHKV2_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO6941676.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLSMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            9399..9611
                     /locus_tag="ACHKV2_003880"
     CDS             9399..9611
                     /locus_tag="ACHKV2_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO6941677.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSLTV"
     gene            9647..10000
                     /locus_tag="ACHKV2_003881"
     CDS             9647..10000
                     /locus_tag="ACHKV2_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6941678.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIAPVQPVQHNIIGDLLRKSRKEARRAGRKQKRWEGACKAL
                     QELNKYRDLINELSE"
     gene            10365..10616
                     /locus_tag="ACHKV2_003882"
     CDS             10365..10616
                     /locus_tag="ACHKV2_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6941679.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIS
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            10662..10946
                     /locus_tag="ACHKV2_003883"
     CDS             10662..10946
                     /locus_tag="ACHKV2_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6941680.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            10999..12309
                     /locus_tag="ACHKV2_003884"
     CDS             10999..12309
                     /locus_tag="ACHKV2_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6941681.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            12306..12884
                     /gene="wrbA"
                     /locus_tag="ACHKV2_003885"
     CDS             12306..12884
                     /gene="wrbA"
                     /locus_tag="ACHKV2_003885"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6941682.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEK
                     AGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            12905..13132
                     /gene="yccJ"
                     /locus_tag="ACHKV2_003886"
     CDS             12905..13132
                     /gene="yccJ"
                     /locus_tag="ACHKV2_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO6941683.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(13170..14411)
                     /gene="agp"
                     /locus_tag="ACHKV2_003887"
     CDS             complement(13170..14411)
                     /gene="agp"
                     /locus_tag="ACHKV2_003887"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO6941684.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(14702..>15070)
                     /locus_tag="ACHKV2_003888"
     CDS             complement(14702..>15070)
                     /locus_tag="ACHKV2_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941685.1"
                     /translation="EFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEKRINEVKNF
                     LTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPPELFAQA
                     YELNNKERNAQIGFLTRYAI"
BASE COUNT         4413 a         3507 c         3596 g         3554 t
ORIGIN      
        1 aaattgctgg atatttcccg tacatcagaa aagtcttccc gctttggttt caacgtaaat
       61 ctttaattaa ccccatcccc gaccacatat cggggattaa attaaatatc tggattaatt
      121 aaccggagga tttgtcatgc tcaaacctca ctacggaacc gcattagttt ctcgcgagga
      181 cgttaaaccc ggcacagcaa ttctttacaa cgggcgttat tacatggcgt cagcgaacgt
      241 taataatgca ctttacgcac attcactgat tgaaaaaatt cgcattatct cagatgcaat
      301 agaagtttac ctgaacaata aaggccaacc gttaatctca ccagcctgaa gggaaatatc
      361 atgctcaatc aaaaaataaa tatcaatgta aaaagcgtcg taactccagc aggaacgata
      421 atgggagaag tttttatgga tgataaaatc atcgcctatt ttgtcgtcct gtctgatgag
      481 gctatttctg ttattgatac ggaaggcaat gttatgttta tcgcggaaca tccagaagat
      541 atcgcattac aggccgctgc atatttcttc gctaaggagc aggaggaaga atgtaactgc
      601 cctgtatgtc agctttcccg acaaattaat ttaatgcatt aaccggaatc aggagttccg
      661 ccatgaacgc atacctcacc tgcgaccgca tagaggagcg tcgctggatt aatcagcaaa
      721 ttcaggatga aaaggataaa tggattgatg atcgggcgca ggagcttatc agcatgttcc
      781 ccgataaacc attgcttatg agcagccttt ttttacccaa agaagcccaa ctagcactca
      841 ctggcgaaaa agctgaagag gcgtacaacg attatatctc agcgatcgcc tatgctcggg
      901 cggaagaaga atgggagaga aaattctccc cctgtccttt ctgattttca ggaccaaaaa
      961 atgttcgata tcgttgaatt tgttaagcag caggagcgct ttttctgcga ggcattaact
     1021 gaaccgacgc tgacatgggc gaaggaaagt cagtttgcaa ttcagcaatt ccagaaaaat
     1081 gcctttctgg ctgacacagc aagggcaaat ctgcccagcg cacagaacgc tatcatcaat
     1141 gttgccgcca tcggcataac cctgaacccg gccagcaagc tggcgtatct ggtcccacga
     1201 aaaaaggctg tatgcctgga tatcagttat atggggcttc tgcatctggc acaggtcaca
     1261 ggagccattc agtgggggca atgcaaactt gtttacgaga aggacattta cgagtccaac
     1321 ggtattgact gcgcccccac gcacaaatac aaccccttcg tagacagggg cgcacgcatt
     1381 ggcggttatt gtgtcgtaaa aacatccgaa ggcgactatc tgaccgaaga gatgagcaac
     1441 agggaaatcg aggtcatcag ggcgtgcagc aaagccggaa ataacggagg aagtagcccg
     1501 tgggatagtt tccccgatga aatggccaga aaagccattg ttaagcgcgc cagcaaatac
     1561 tggccccgtc gcgatcgcct ggatacagct atcgactacc tgaacactca gggcggtgaa
     1621 ggtatcatcc tgaatgctga tcacatccct gagcgtgacg tcactcccgc atcagatgag
     1681 attatcaatg agatcactca ggcaatcacc gaaattaaca agacatggga tgacctgctt
     1741 cccttatgtt ccaaaacatt ccgtcgcacg attgcatcac atgaatatct cagtcaggaa
     1801 gaagctgtca aaacgcttga ttttgtcaaa aagaaagctg ccagaaacaa ggccacggcg
     1861 gaagcgaaaa ttcacgccac cacggaaaat aacagcgagg ccgtgtcatg acaccagaaa
     1921 ttattctcca gcgaactggc gtggatatta ccagcctcga ccagggcgat gatggatggc
     1981 acaagctgag actcggtgtt atcactgctt cagaagttca caatgtgata gcaaagccac
     2041 gttccggcag caaatggcct gatacaaaaa tgtcatactt ccacaccctg ctggctgaag
     2101 tttgtactgg cgtggcaccg gaagttaacg ctaagtcgct cgcatgggga aagcaatacg
     2161 aagatgatgc ccgtgccctc ttcgaattta tcgcggatgt taccgtctcg gaaacgccaa
     2221 taatttttcg tgacgaaagc atgcgcaccg cctgctctcc cgacggttta tgcagcgacg
     2281 gtaacggtct tgagcttaaa tgccctttta cttcccgcga cttcatgaaa ttccggcttg
     2341 gcggctttga cgctatcaag cctgcttaca tggcccaggt gcaattcagc atgtgggtta
     2401 cagacaagga cgcctggtac ttcgccaact acgacccacg catgaagcgt gaaggcctgc
     2461 attatgtcgt ggtcgagcgg gatgaaaagt acatggcgag ttttgatgag atggtgccgg
     2521 agttcatcga caaaatggac gaagcactgg cggaaattgg ttttgtattt ggagaacaat
     2581 ggggggttaa taactaatgg atgaagtgat ttttacttat aacgaagaat cagcactgac
     2641 cgccggacaa ggtagtttta ttaccgaaac gggtgcgcat atcattaaca tcaccgaagc
     2701 agaactcaag caatcagaaa aaggtgcccg attcattgag ttttctggag aatccgacga
     2761 cggacggaaa atccaatatc tcagtgtttg tgttcagaag aatgacggta ctgaaaataa
     2821 atttggggcg agcattattc acgccatgat gggatgcaca ggaattgggc agttaacgca
     2881 acatatggtt tccgtcagta aatatgttgc acctgagttt cacggaaaga aaatcggact
     2941 cgtcctccag aaagtattaa ccacaaacag aaagactggc gcagacggtt accagatgga
     3001 aatccgcatt ccattcattg cagaaacagg gcagacactg aaagaaaaag ctgaaggaaa
     3061 gaaaccagaa acggtagcaa atatggtttc cactctcaaa gacaaagaca atcgcagcaa
     3121 aaacgtaaac ccgaatcaca cggacgaccc aggttactgg cagtacggca gcgatagttt
     3181 ttaattcaca aacaaaacca ggctttaaat gcagtgaaca actgaagcct taacacacct
     3241 ctacacggag atattaatat gaaccagcat caaactgatg ttaatgtttt cattaacgac
     3301 ctcgacggcg gggtatttgt taacaaactt ggcgcggtat taagtgaagt tgcctttggc
     3361 gtaaacagca caaacaaaaa aggaaaggta tgtgttgaat tcgaattatc ttcacttgat
     3421 gaaaatcgcg tatcagtttc ccataaacta aaattcacac gcccgacaat gcgtggtagt
     3481 aaatcagaag aagacacaac taacaccccg atgtttgtaa ataaaggtgg tgagcttact
     3541 ttgttccaga aagaccaggg acagcttttt gataaacagg gccaacatga cgctgtttta
     3601 cgctgaatat tccccgcctt aaaacgctcg cgcattatcc ctaaatacat aaattaaagg
     3661 taaatataca tgtctcagtt agatagcaat gccattaaag aaattgtaaa actcaccact
     3721 actgcttttt ctggtgaaaa tttgccgctt acggaatgcc ctgttgcgtt attgccggac
     3781 aatgtaaata tcgaaagtct ggaacgattc atgacagaac gttttcgttt ccgtggagtc
     3841 atgaccacaa ccagtattga tgactttgtt gaatacagca aagggtatgc cgatgagcac
     3901 tcccgctgct tcattaacgc ggaaacgatg aaagcagtca ctgtgttcaa tatcggcact
     3961 ctggagcaac ctggacacgc tgacaacaag gcactgctgg aactgaaagc cacatcacca
     4021 tatcgcgcat tgcgtgacgt tgacggcaaa aaacaactcc agaaatctct cgctgaatgg
     4081 ctggaagact gggccgactt cctcacagcc tatgacagcg acggtaatgt gctggatatc
     4141 aaacaggcta tctctgctgt ccgtcgtctt accatcgatg caaaacgcag tgctgaatat
     4201 gaagagcaaa acttcagcgg tagccgctca gtcatggagt ccgtggaggc taaaagcaaa
     4261 gaaatcatgc ctgccacttt ccgctttgaa tgcatcccat acgagggcct gggcaacagg
     4321 gaattcacgc tgcgattaag cattctgaca agcgaacagc ctgttctggt gctacgtatc
     4381 gtgcgtgttg aagctgcgga agaagaaatt gccaaagagt tccgtgacct gctgaaagag
     4441 cgtttcgaag aagaagatat cttaaccttc atcggcacgt tctcagtgtg atgaaatctg
     4501 tgacagggac gtcacgacat atcgctcata tttcacgaga aaatacacag ttcttggagg
     4561 gtatactatg caatttaaag atttaccagc ggatattcag aaaatagctg cggatacgct
     4621 taaagcccat ttatcggtgc ttaatttaat aaaggaacca aagacgaatc tggaaaatat
     4681 ttcccgtaac gtgcgagatg tttttgtcgg gctgtatgct tatgacaatg aaaagcacga
     4741 ggaacatatt caaaatggtt gcattaataa atgtcaacaa catatcaaac ttcctgttgc
     4801 tgataaaaca gagcagaaaa cagaactatt agtattctgg aataccgttg aggctattgc
     4861 caggagcaag atgtttgagt ttaacaacat caatcattca gggatcgaca ctcaccaatc
     4921 atcacatcgt gagggggtgt ccatgcttct tgctctgttg tgagagcaag gcgaatgtcc
     4981 tcagccgaca tcaaacgata ctgagcaata gtctattctc gtacgctgca acgtgaggtc
     5041 gtgcgccgga cacgggtaaa catccggcaa ttccagctta caaccaattc ccctcccata
     5101 acgagacgaa aatatgacaa ccgaaattga ctatcaggta ttgcgtgacg tgtcggaacg
     5161 cgcaattaca gcgatgacac atctgtcaat attaccaggt gatgatgatt tattaagcga
     5221 aaagaagctc aaggagcttg gtattgatat tgatgcgatt cacgccttta aaattatggc
     5281 cgggccagaa accgtgctgt cactactgga tgaacgagat gcattaaacg aacgcatagc
     5341 cgaactggag gctaatttag cgaagctggc cgaagaccaa cagaaagcga ttgagtcaat
     5401 taagcaggct gattcggctg ttaagttggc acacgagaag ttttcggctc tggcggcgga
     5461 gaatgcgggg ctgaataaat ttatcgcaca gagttgctac gtgtttgatg gcgagcagga
     5521 tgagctatct gatgcgtata tctgcgcaac agatggaggg atgccgcaaa ttccagccac
     5581 cgatgctttt ctggctgaaa ttcgtgcgga ggctcgcaac gaggggatta actataccgc
     5641 aagccgtctt gctgctgctt tcaaccacgg atttatcaat aagtctttac gtgaagtttt
     5701 cgacgttacg cgcatgattc tgtcagcaaa agaagagtta gctaatgaat cgcatccgat
     5761 tgatggcctg tccggtgaat atgcagagaa atcccttgaa gaatgggcgg aacggcttcg
     5821 caaaggaggc aaccagtgag caagattgac tatcaggcac tgcgtgaagc ggcagagaaa
     5881 gccggtgaag ataagtggca ggctaaaaaa ataaatggtg attttttcgt tattcgtcac
     5941 ggtagttata caagacagca tggctacaca tcgtatcaac ccattgcgga gattgattgt
     6001 aagccagtcc gggattttgt tgccaaggct aatccggcta ccgtgctgga attactggat
     6061 gaactggaag cagcaaaaaa gcgcattgca gaactggaag cgcgggaaat actgctcccg
     6121 gaacgtagca gcatgcttca tcgaacagat tttcacgatg attaccaaac ggtaatggca
     6181 tacaaagttt ctgaagtcat cgatgcaatc cgcgctactg gcattcgcat caaaggagag
     6241 tgagatgaac ggacaaatct caattgttcg accaggagca tgtgacgata gcgagataca
     6301 catgattatt cgtctggcga gggggaaaac aataactgtt ctcactactc cagaaaatct
     6361 cgcattagca ttaacaggaa agtcagacct gccagtagag ctaaagctgc gaaatgttga
     6421 gattaaggtg aaatagctat gaccactttt accaaagatc gactgctgac aatccagcat
     6481 tggcgcgaaa catacggacc gggtagcaac gttgtgcttc cagcagaaga agcggaagag
     6541 ctggcacgga ttgcgctggc atcgctggaa gcagagccgg tggcaaagat tatagctcat
     6601 tacccattag gggttgacgt aggcaaacaa aaattcgtac aggccattag agagcttcct
     6661 gactttggcg gatatctatt tgccgcccct ccagcgccga tagtgccgga agaaatgtat
     6721 tggcaggatg cgccagttga aggcagcagc aaagcggctg catacgctac aggctggaac
     6781 gattgccgcg aagccatgct tcagtccgga aactttcggg aaaataaaga ttcgtcaacc
     6841 aataattttc ggaaaatccc ggaagcgtca accagctctc cggtaactcc ggctcttctg
     6901 cctggtggtt tcaccattga ggaggcgaag gaattacatg aagacctggt acgcagccac
     6961 ataagcaagg ccttaagtgg cgaaaagatg aaaaagaaag atcgcgatgc tgatttgcgc
     7021 tggattcatg gcgttatagt tcaggcagcg tggtttgtaa aagcatcact ggagcagaat
     7081 gcactatcgg gcaactatcc ggtaactccg gatagttgga taagctgtag tgagcgaatg
     7141 ccgaaaaaga atcagaacgt acttatttcg gtgaatttcg atagtgatct ggttgagccg
     7201 ctaatatgct ccgcacgcta taccggaagc acctttcggc gaggagaagc aacgattaag
     7261 ccgggcaatg gtgttgagca ggtaacacac tggatgccgc taccagaacc gccgcaggag
     7321 gttaaccgtg gctaacctgc aacttgccgt caaaggtgaa tacttcgcag atagtttccc
     7381 ccaaatctgg ggaaaagccc gaatggcgcg gcttacagca agataaggcc tacatgattt
     7441 gacaaatccg ccagagctat cgcatactga ccgcactaga acttcaaagc ggtcatccgc
     7501 acccgatagc tttgcggctt ttttatgcct gcaatttggc atagtcacat ccgtacaaag
     7561 gtcgggtgga gaggcgtaat acaatacccg caaggggaat atgcccggag catctttgaa
     7621 ggctctagtt gacacccgat caccagccac taactggtga ttgctaacta aaacttcaaa
     7681 ggaggtcatc atgaccagtc aactcatccc cgtattcaac ggcactatcg acaacgaaac
     7741 aattcttcta gttaacgctc gtgatctaca ctcttttctt ggtgtgggca gaatgttcgc
     7801 gcactgggtt aaagaacgca ttgctgaata tggattcatt gaaagccagg actatatttt
     7861 gatttgccaa aatgggcaaa ccaaagggcg aggaggtgat cgccgtagca aggactacca
     7921 cctaactctc gacacagcca aagaactggc gatggttgag cgtaacgaaa aaggccgcca
     7981 gatccgccga tacttcatcg agtgcgaaaa gaaacttcgc cagagccttt tacctgcacc
     8041 aatgaacatc aactaccctc tatcgtggtt ttcagagcac cacccctact ccatgatgag
     8101 ctatgttgat cgaaaaactc ttaacctgga cgtttctgtg ctcttcgaca tgccaagccc
     8161 aactatgcgc atcctcaatg agctacacag caaaggctat aacgttgacg ccgctgtcgc
     8221 tgaatttaac gccttcaagc atctgacgga agaaatgcgc cgcacgctac aggatatttc
     8281 aagattgtca gatcgaaatt cccgaaaagg cttctcgtta agcctgtaac ttcccctacc
     8341 atccccgaca tcccgtcggg gttttcatat ctggagacaa aaatttgaaa cagatcgctt
     8401 tctacaggcg ttctggaagg cccggtgcct tccgtgggtt aaaagaacgc gtcacctgga
     8461 tgattcagtc tcgtggtcgt cctgttactg gcagtgaaat agcagagaaa tttggtgttt
     8521 cactttgcga gttcaacaag gtagctcgcg gcctgacaaa gggcagtaag gttgtgaaaa
     8581 taaaggcttc agaaccattc accacagaca ccggaatcgt tgatcgcttt ttctccctcg
     8641 aatcaaatcc tcgtcgtgac acacctcgct cacgcaatgc cgttcctcca ttcagtcgca
     8701 gaagccgtga acacgcagca aaaaactgtc gcgaggaata cgtgcaaaag gccgaacgcc
     8761 gtcgccgact gattaaagca ggactttaca ttgatgagtt tgaaaacgcg ctatgacgaa
     8821 aaaatacacc ctcatttacg cagatccccc ctggacattc cgcgacaaag caaccgatgg
     8881 tcaacgcggt gcaagtttta aatatccggt catgagtctt ctggatatct gccgcctccc
     8941 ggtatgggaa ctggcagccg ataattgcct gttgtctatg tggtgggtgc ctacacaacc
     9001 acttgaagca ttgaaggttg tagaagcgtg gggctttcgt ctggtgacga tgaaaggatt
     9061 aacctggaac aaatgcggga aaagacagac cgacaagctg gtcatgggta tgggtagcac
     9121 cactcgcgct aacagcgaag actgcctttt tgcagtgaaa ggaaatctgc ccgaacgcat
     9181 taacgccgga ataatccagt cattcactgc accgcgcctt gatcactccc gcaagccgga
     9241 tatggctcga gaaaagcttg tgcaacttct tggcgatgtt ccccggatag aactgttcgc
     9301 ccgccacacc tcgcatggat ttgatgtatg gggtaaccaa tgcggcacac catccattga
     9361 gatggttccg ggtattgtta aatttctgga gaaaaccaat gagcgaaaaa acgacgttga
     9421 caaaggcatc accagttgaa ttaaggcagt gtctggaaat cgcaaatcaa cttgccagaa
     9481 gtggaatacg atttgttcca atcccgatta cagcagatgc agaacttcat ctgtttggtg
     9541 aaattctttc ccgaaagctg gatgaactgg aaaagctggt agaagaagct gacacctcac
     9601 taaccgtata acagccccac cgacattaaa atatcaggag aaaaaaatga acgcagtgct
     9661 cacagaattg aacaaattag gaaaagcatc agccgaaagt atttctaaag gtctcaatat
     9721 tgatttgaat gacgttattg acactctatg gaagttaaaa aaccaggggg tagtaactgt
     9781 aaaaaatggc atctggcagg cagttgcaag ggaagtggac aaaaaaccaa atatcgcccc
     9841 agtgcagcca gtgcagcaca acattatagg tgacctgcta cgtaaatcac ggaaagaagc
     9901 gcgccgcgcc gggcggaaac agaaacgatg ggagggtgca tgtaaggcgt tgcaagaact
     9961 gaataaatac cgtgacttga tcaacgaatt gtcagagtga gcgaatcaca gagagaatat
    10021 tttgagaaac aacccgcgta cattaatatt gtttttcctt tctctaatga cgggcggtat
    10081 cctgttattc gcaggaacaa cattatttat ttttgttgcc cggctaactg cgaggataat
    10141 gacatgaaaa tcacatttga aagttacggt ttaacagcaa gtgtggttat atccagctct
    10201 atattcgaaa ggcgcaaaca tcagcatatc gttgatgcag tgaaactcaa agccccggaa
    10261 gttaccgtca caacacatgg acttttcagg atacgaacga cgttgactac aacacacctc
    10321 tcggcatgcc gtgtttatga tatggcactc aaggaataca accagtgttc gtcctgatcc
    10381 agcgcgggca gtctttcgtt gatgccaaca actatccggt agaaatatgc aaggtaactc
    10441 tgactcaggt gatctaccga aggctcgacg gcagaaccag agccacttca attagtgcat
    10501 ttaatgaaga atttgagcga atcgagcaca acgaactgca tatgattaaa gcggaaattg
    10561 agaaggaaaa gcatattgcc agccttcgaa aaatgcgccg tacatcaatc aactgacaac
    10621 cgccttcggg cggttttttc ttacacatgc agaggcaact tatgcgcgag ttagtaaacc
    10681 aacataacca tggcattcag ccagtcatca cacctgttgt acagataaat gcgaatgaat
    10741 gggtaacact ggagctttta atggctgtaa caggcctgag aaaaggaaca atattacgcg
    10801 ccagggacag tgcgtggatg aacggcagag aatataaaca aatcgccccc gacggaacgc
    10861 caaagaaaaa cagcgaatgt ctctatcacc ttcctaccat caacacttgg atcaaaaacc
    10921 aacccttacc atctcaggat gtttaattct tgtccataag agtataacct gagcgtgctc
    10981 ttggacgcag gaggaacaat ggcgaattca gcctatccag ccggcgttga aaatcacgga
    11041 ggaaaactcc gaataacgtt taagtacagg ggtaaacgag tgcgcgaaaa tcttcgcgtg
    11101 cccgatactc cgaaaaacag aaagatcgct ggtgagttaa gggcttctgt ctgctttgca
    11161 atcagaacgg gaacgtttga ttatgccgag cgattccctg actcacctaa cctgaagcta
    11221 tttggcctgg taaaaaaaga tatcaccgtc ggtgaactgg cacagaaatg gcttactctg
    11281 aaagcaatgg aaatcagtag taacgcctta aatcgttatc aatcagtgat gaaaaatatg
    11341 ctaccgaggc ttggtcctgg caggctggcg tcatcgatta caaaagaaga tctgctgttt
    11401 atcaggaaag atttactgac cggggaaaag ggaagcagga aaaccagcac gtcccgaaaa
    11461 ggaagaaccg tacccacagt gaactattac atgacaacaa cagccggaat gttcagcttt
    11521 gccgccgaaa acgggtatct ggagaaaaac ccgtttaatt caataacacc gctgaggaaa
    11581 tcaaaaccag tgccggatcc actgaccaga gatgagttta gccgtctcat tgatgcctgc
    11641 catcatcaac agaccaaaaa cctctggaca gtggctgttt ttacagggat gcgacacggt
    11701 gaaattgccg cacttgcatg ggaggatatc gacctgaaag ctggcacgat aacagtgcga
    11761 cgaaatttta caaaaatagg tgattttacg ctaccaaaga ccgacgcagg cactaaccgg
    11821 gttatacatc ttctggcacc agcaattgaa gcacttaaaa accaggcgat gcttactcgt
    11881 cttagcaggc agcatcagat cactgttcaa ttacgcgagt acggaagaac aattttgcac
    11941 gagtgcactt ttgttttctg tccgcaaatc gttcgcaaga atcacaaggc tggtattaac
    12001 tacgcggtaa gctccatcgg agcgacatgg gattcagcaa taaaaagagc gggtatccga
    12061 tcccgtaaag cgtatcagtc acgccatacc tatgcgtgct gggctttatc tgccggagca
    12121 aacccgacat ttattgcatc acagatgggg cactccagcg ccagcatggt ctacaatgtt
    12181 tatggtgcat ggatgcctga gtgcagcgtg actcaagttg ccatgttgaa taatgtcctt
    12241 aatgcccgtg ccccagacgt gccccaaagt gaccaggagg atgaaataaa attatatttt
    12301 tcaaaatgat aaaccatatc cttagacatg ttgaaacgat ggcacgcgca gtcgctgagg
    12361 gtgcaagcaa agtggatggc gctgaagttg tcgttaagcg tgtaccggaa accatgtcgc
    12421 cgcaattatt tgaaaaagca ggcggtaaaa cgcaaactgc accggttgca accccgcaag
    12481 aactggccaa ttacgacgcc attatttttg gtacacctac ccgctttggc aacatgtccg
    12541 gtcaaatgcg taccttcctc gaccagacgg gcggcctgtg ggcttccggc gcactatacg
    12601 gaaaactggc gagcgtcttt agttccaccg gtactggcgg cggtcaggaa caaaccatca
    12661 catccacctg gacgaccctt gcgcatcacg gcatggtgat tgtccccatt ggctacgcag
    12721 cgcaggaatt atttgacgtt tcacaggttc gcggcggtac gccgtacggc gcaaccacca
    12781 tcgcaggcgg tgacggttca cgccagccca gccaggaaga actgtctatt gctcgttatc
    12841 aaggggaata tgtcgcaggt ctggcagtta aacttaatgg ctaatcttca acaggaggat
    12901 acgcatgcca actcaagaag cgaaagctca tcacgtcggt gaatgggcat ctctgcgcaa
    12961 tacgtcgccg gaaatagccg aggccatttt cgaagtcgcc gggtacgacg aaaaaatggc
    13021 ggaaaagatt tgggaagaag gtagcgatga agtgttagtc aaagcctttg ctaagaccga
    13081 caaagattcg cttttttggg gcgaacagac catcgaacgt aaaaacgttt aaccagcgac
    13141 tcccccgtag cgaacggggg agttttctgt tatttcaccg cttcattcaa cacgctatca
    13201 aacttatcca tcgggcagaa accatcagcg tctatcgggc aaccgcttaa ttccagcgtc
    13261 acgcgctgcg caggtgcctg cagggttaac gcatcggcat tacgtaactg ttccgcactc
    13321 tggtacacat attcaatttt catcaaatcg cgattggctt tgctgtcacg ccaacgctgg
    13381 aaaacgattt tgccgccaat cggcgtgcgt tcgttctggt catgcaactg atacggtttg
    13441 aaatccagcg ccgttaacag agaggcaatg ttggagtcat gcccaactaa caccgtgatt
    13501 tttggagcac tggtgcgatc ggtgaccagg gctttgtcga tataactgac cagcggtttc
    13561 gcaacattgc gcgccacttc cggtgaggta aacaggctgt cctggtaacc gtttttcagc
    13621 ttcgacaaca ccttccactg ctggtcagat ttgatttctc cccaggccac ctgatccatc
    13681 ggaaaacctt cgtagtattg caaagtaaac gcatctacca gcgagttgcc gactttcagc
    13741 ggcccggaaa cacctggttc ttgttgatac ttggcgctaa aggtattttt gccatccacc
    13801 agcgaacact gttgtttctc tttacaggca ggggaatctt tatagttaac gattttttcc
    13861 agtagctggt agctgtcggt aagctggagt ttgctgagct ctttctccat tgccgccacc
    13921 gcctgttcac tgaatgcggc ggaatcatcg gtgatcaccg ggttaaaggt tgggtccata
    13981 gtgcccattt tttcctggtg atgcacagga atatcacacc ccgggaatgc gccggtaata
    14041 aagaactgtg cggtcgcaac ggtacgttgc agactattgg cataggcata aacggtgtcc
    14101 ggcggcgggc attcccccga ttttaccatc ccctgctctg ccagccattc acgcatgtaa
    14161 tggcccatat acacttcgag tacgccacct ttggtggtga gttgcccacc ggggacgtcc
    14221 cattcgggcc atttattcgg cgtcgactgc tccagcacac tgccattgtt cgccagcggc
    14281 gcacgtaagt tatggcggct catcatgagc acttgctgta gctgatagcc ttccggtacg
    14341 gtttgtgcct gagcgtttga agcgagtaaa actacccctg ccacagctgc ggcgattagc
    14401 gttttgttca ttcctggcac ctcttttgtt atcaatcact aaagagtgtg acagaaatat
    14461 gacttttttc cgggaacagg ttcgcaaaat ggcttgttga acggctgttt ttatattcat
    14521 ctttaaaaac aggcaaggtt tgacgcaaac tatgcctgat tagcccctgc ctcgcgtacg
    14581 ccgctgatga ttgcacctgt ggctaaacag aatatcggcg gcgttaccaa tacagaggca
    14641 atatttacca ataaaaaagt gtgagactaa taacaatggt ttttagcata tgcctctgtc
    14701 attaaattgc ataacgagtt aaaaaaccaa tttgcgcatt cctttcctta ttgtttaact
    14761 cgtaagcctg agcaaaaagt tcaggaggga ggtaaacgtc ataatgattg ccagtgttta
    14821 ttaaataacc atactcaaga tcttcatctg ttgaaaagga ttcattccct aaggagattg
    14881 atgccacatt actattaaca ataaataatg agcaaatgct atctgaaaga gcagtgagga
    14941 aatttttcac ctcattaatc ctcttctctg ggtcggtgtc taaaacaagg cgcgccttgt
    15001 tatcattaaa tataaactcg ggtaaatgca gtatgcattt tttgtcattc aagcaactat
    15061 caataaattc
//
LOCUS       DAZFTN010000111        14884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFTN010000111 DAZFTN010000000
VERSION     DAZFTN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            140..382
                     /gene="yjbE"
                     /locus_tag="ACHKV2_003889"
     CDS             140..382
                     /gene="yjbE"
                     /locus_tag="ACHKV2_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO6941686.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            496..1134
                     /locus_tag="ACHKV2_003890"
     CDS             496..1134
                     /locus_tag="ACHKV2_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO6941687.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1131..1868
                     /locus_tag="ACHKV2_003891"
     CDS             1131..1868
                     /locus_tag="ACHKV2_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6941688.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLGGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1868..3964
                     /locus_tag="ACHKV2_003892"
     CDS             1868..3964
                     /locus_tag="ACHKV2_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6941689.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4011..4289)
                     /locus_tag="ACHKV2_003893"
     CDS             complement(4011..4289)
                     /locus_tag="ACHKV2_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941690.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4290..4433
                     /locus_tag="ACHKV2_003894"
     CDS             4290..4433
                     /locus_tag="ACHKV2_003894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941691.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4559..4969
                     /gene="psiE"
                     /locus_tag="ACHKV2_003895"
     CDS             4559..4969
                     /gene="psiE"
                     /locus_tag="ACHKV2_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO6941692.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHKV2_003896"
     CDS             complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHKV2_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO6941693.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6860..7750)
                     /gene="malG"
                     /locus_tag="ACHKV2_003897"
     CDS             complement(6860..7750)
                     /gene="malG"
                     /locus_tag="ACHKV2_003897"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO6941694.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7765..9309)
                     /gene="malF"
                     /locus_tag="ACHKV2_003898"
     CDS             complement(7765..9309)
                     /gene="malF"
                     /locus_tag="ACHKV2_003898"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO6941695.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHKV2_003899"
     CDS             complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHKV2_003899"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO6941696.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11018..12133
                     /gene="malK"
                     /locus_tag="ACHKV2_003900"
     CDS             11018..12133
                     /gene="malK"
                     /locus_tag="ACHKV2_003900"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO6941697.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12205..13545
                     /gene="lamB"
                     /locus_tag="ACHKV2_003901"
     CDS             12205..13545
                     /gene="lamB"
                     /locus_tag="ACHKV2_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO6941698.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13696..14616
                     /gene="malM"
                     /locus_tag="ACHKV2_003902"
     CDS             13696..14616
                     /gene="malM"
                     /locus_tag="ACHKV2_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO6941699.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3664 a         3836 c         3925 g         3459 t
ORIGIN      
        1 tttgatattc attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc
       61 atgctttagt tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat
      121 tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct
      181 tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc
      241 aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt
      301 gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac
      361 cacgaccacc agtacccagt aataaagtat gtatccccaa aataattcga gtcattgcat
      421 ctgtggctag aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc
      481 ctctggagaa gagtcgtgaa gcgacctgca ttcattctta tctgcctgct attacaggcc
      541 tgttcagcca cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca
      601 ggcgtacagc tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag
      661 cttaatggtg ggccgcagtt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa
      721 tgggtcactc aggatcaggc cactctcgtc acacaacatg gtcgtctggt gaagactttg
      781 cttggcggcg acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc
      841 gcacaaatca ttgatggcgc aacctggatg cgcacgatgg gctggaccga gtaccagcag
      901 gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg gcaccggtac cgttaaggtc
      961 ggcagcgatg aaaccgcggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc
     1021 tggcataacc gctattggat cgacagcgga gggcaaattc gccagtcgga acagtatctc
     1081 ggcgcggatt atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac
     1141 aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg
     1201 tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct aacgggcgca gagcatttaa
     1261 tcgatctggt aggccaaccg cggctggcaa acagctggtg gcctggtgcg gtgattagcg
     1321 aagagctggc aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag
     1381 aacagggcgc agattccagc gccgacgatg ccgctgcgat taacgcctta cgccagcaaa
     1441 ttcaggcgtt gaaggtgacg ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg
     1501 ttgctgaacg cgctaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccac
     1561 cgtccacggt cacgttgttc gggcttatca gccgtcctgg caatcagcca ttcactcccg
     1621 gtcgcgacgt ggcgagctat ctctccgggc aaagtctgct tggcggtgcg gatcgtagct
     1681 acgcgtgggt ggtttacccg gacggacgca cacaaaaagc gccggtggct tactggaaca
     1741 agcgtcacgt agagccgatg cccggcagca ttatttatgt tggcctcgcg gactccgtct
     1801 ggagtgagat gcctgatgcc cttaacgccg acattcttca gactctgacg cagcggatac
     1861 ctcaataatg aaaaaaagac atctgcttag cttactggcg ctgggcatta gcacagcttg
     1921 ctacggcgaa acatatcctg cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg
     1981 attattacaa acgcccacgg ctcgcatggc gcgggaaggg gagttgagtc tgaactatcg
     2041 cgataacgat cagtaccgtt attactcagc ttcagtgcaa ctcttcccgt ggctggaaac
     2101 aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg
     2161 cgatcaaacg tataaagata aagccttcga tctcaaactg cgtttgtggg aagagagtta
     2221 ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc
     2281 ggaatatctt gttgccagca aagcctgggg gccgttcgat tttacgcttg gcctgggctg
     2341 gggatatttg ggcacaagcg gtaatgtgaa aaatccgctc tgttcagcca gtgataaata
     2401 ttgctatcgc gataacagct acaaacaggc gggatctatc gacggtagcc agatgttcca
     2461 cggtcctgcc tcactgtttg gcggcgtgga ataccagacg ccctggcaac cgctgcgcct
     2521 gaaactggag tatgaaggca ataattatca gcaggatttt gccggtaagc tggagcaaaa
     2581 aagtaagttt aacgtcgggg cgatttatcg cgttaccgat tgggccgacg ttaaccttag
     2641 ctatgaacgt gccaacacct ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct
     2701 gcgcccgtct tacaacgata acgcccgccc gcaatatcaa ccgcagccgc aggatgccat
     2761 tttgcagcat tcggtggtgg cgaatcagtt aacgctgttg aaatacaatg ccggacttgc
     2821 cgatccgcag atccaggcga aaggcgatac gctgtatgtt accggcgagc aggtgaaata
     2881 tcgtgattcg cgcgaaggga tcgttcgtgc taatcggatc gtgatgaatg atctgccgga
     2941 tgggatcaag acgatccgcg ttacggaaaa tcgccttaac atgccgcagg tgacgacgga
     3001 aacggatgtc gccagcctga aaaatcatct cgccggagag ccgttgggcc acgaaacgaa
     3061 gctggcgcaa aaacgcgtcg agccagtggt tccgaagtcc accgagcagg gctggtatat
     3121 cgacaaatcg cgctttgatt tccatatcga tccggtgctg aaccagtcgg tcggtggccc
     3181 ggaaaacttt tacatgtatc agctgggcgt gatgggaacg gcagatttgt ggctgacgga
     3241 ccatctgctg accaccggca gcctgtttgc aaatcttgcc aacaactacg acaagtttaa
     3301 ctacaccaat ccgccgcagg actcgcactt accgcgcgtg cgtacccatg tgcgcgagta
     3361 tgtgcagaac gatgtctatg tgaataacct gcaagccaac tacttccagc atctgggcaa
     3421 cggcttctac ggtcaggtct acggaggtta tctcgaaacc atgtttggcg gtgcgggggc
     3481 agaagtgttg tatcgcccgc tggacagcaa ctgggcgttt ggtctggatg ccaactacgt
     3541 taaacagcgc gactggcgta gtgcaaagga tatgatgaaa ttcaccgact acagcgtgaa
     3601 aaccggacat ctgaccgcct actggacgcc atctttcgct caggatgtgt tagttaaagc
     3661 cagcgtcggg cagtatctgg caggggataa aggcggcacg ctggagatcg ctaaacgctt
     3721 tgacagcggc gtggtggtgg gcggctatgc cacgatcacc aacgtttcga aagaggagta
     3781 cggcgaaggg gacttcacca aaggcgtgta tgtctctgta ccgttggatc tcttctcgtc
     3841 tggcccgaca cgcagccgtg cggcgattgg ctggacgccg ctgacgcgtg acggtggtca
     3901 gcaacttggg cgtaagttcc agttgtatga catgaccagc gaccgtagcg tcaatttccg
     3961 ctaagtcatg ggaaaggtgc cagttttcgc aatcactggc acctttattc ttaatggcct
     4021 gttgagccat caggaacgca gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa
     4081 cattctgtct tcctgtccgt cgatatcggt tgcaggtctg gttgcctgag cgactgggcc
     4141 atcattgggc tggagacatt gctttgcggt aggtcggctt tatcagcagt accgagcgga
     4201 ccagcataag caggaagagc agagactgat aacatcaaag cagcaaaata aggcttcatt
     4261 tttaccacct ttatcaggtt acgtttcatt tgttccagag gaacattgtc gatttttcgc
     4321 gcattgctgg tggctgggaa tcacctgaat gggtgatttt tgaattaccg gctttggtgc
     4381 ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc ggcctacggg taggtatatc
     4441 cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa aacatacaca aaaaatatag
     4501 atctccgtca catttttgcg ttatacagga agctcgccac tgtgaaggag gtactgctat
     4561 gacgtcactc tctcgtccgc gcgtggagtt tatctccacc attttgcaga ccgtactcaa
     4621 tcttggcctg ctgtgcctcg gccttatttt ggttgtcttc ctcggcaaag aaacggtgca
     4681 tctggctgat gtgctgttcg cgccagaaca agccagcaaa tatgagctgg tagaaggact
     4741 ggtggtttac tttctctatt tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg
     4801 ttttcacttc ccgctacgtt actttgtcta tattgggatc accgccattg tgcgcttgat
     4861 catcgtcgat cataaatcgc cactggatgt gttgatctac tcggctgcga tcctgttact
     4921 ggtggtcacc ctgtggctgt gtaattcgaa acggctgaag cgggagtaaa aagacagcac
     4981 gccgaactgg cgcggcgtgc tggacaggaa gattacagcg tagcagtttg ttgtgttttc
     5041 ttcgtttccg gttcccagag ggcttccagc tcctcaaggg ttttaccttt ggtttccggg
     5101 acaaatttcc acataaacag tgctgccaga acgcccatac aaccgtaaat ccagtaggag
     5161 aaaccgttgt ggaaatgggc caccagccag gagtttttgt ccatcatcgg gaaggtccag
     5221 gagacgaagt agttcgccag ccactgggcc gccaccgcga ttgccagcgc tttacctcga
     5281 atagcattcg ggaagatttc cgacagcagt acccagcata ccggacccca ggacatggca
     5341 aaggcggcaa catagaacag catcgacagt agcgccacaa tacccggtgc ctgagtgtaa
     5401 aacgcggtac cgaggctaaa cataccgatt gccattccga gtgcgccgat aatttgcagt
     5461 ggcttacgac caaatttatc caccgtcata attgccagaa cggtgaaggt gaggttgata
     5521 actccgacaa taatggtctg caacagcgcg atatccgtgc tggcccccag cgttttgaac
     5581 acttccggcg cgtagtacag caccacattg atgccgacaa attgctggaa gatggagagc
     5641 ataacgccga tgacaatcac gcccacgcca aacatcagca gacgaccacc ggttttgcgg
     5701 ccatgatcca gggagtgttt aatttcctgt actgcctgag ttgcaagcgt gttgcccata
     5761 attttgcgca ggataccttc cgcctgttct tgcttgccgc gtgacatcag ccagcgagga
     5821 ctttctggca cggtatacag cagcattaag aacagcagtg cagggataca ttccgaggca
     5881 aacatataac gccagccgtc agtattcagc cagctggcat caccggaacg cgcaataaaa
     5941 tagtttacgc agtaaactaa aagctgcccg aaaataatcg caaactggtt aaatgaaacc
     6001 agtttcccgc gaatatgagc aggagcaagt tccgcaatat acattggtga aagcattgag
     6061 gctaaaccaa cgccaatacc gccaataata cgataaataa caaattccgg gacataacct
     6121 gccagataaa caggcactgt gttgtccggg tttatagagg taaaaccaag ttctggccag
     6181 gcagaaccta caccagaaat aaaaaacagg acagcagcaa tcttaagtga atcacgacga
     6241 ccgaagcggt tactgcaata accaccgagg gcaccgccga tgatgcaacc aatcagagcg
     6301 ctggccacgc aaaaccctaa cagggagttg gcagcggatt cacttaagtt ttgtggagca
     6361 acaaagacgg tattgagtga ctcaacagta ccggaaataa cggcggtgtc gtagccaaat
     6421 aataaaccac ctaatgtagc gactaaggta atcgaaaata tataactgga attatactgg
     6481 gtattcattc agacctgcct tagaccattc tgatctttta tcagtgcatt gatgttggaa
     6541 ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg tttacgtaat gtcttttctg
     6601 tgatcttaat tgtgataatt atccaaaatt gataaaaaca atactattgc cgtgactcag
     6661 agcacgaaag agaattatcg taagtgggaa aacaaataac gtaaaaataa taagctctat
     6721 gatggaaata ttaaccggcg aacgattcag attgcagacg aaagaaaaaa aggcgctccg
     6781 tggagcgccg aataacagtc acaagttggg ataacgtaag ttgagggtgc agcggcataa
     6841 cattggcaga acaacatctt taacctttca caccacctgc cgtcaggccg ttgaccagcc
     6901 agcgttgagc cagcaagaag acgatggtga tcggtaatgc agacatcacg gcagcggcgg
     6961 caaagtcacc ccacaggtag ttttgcggat tgaggtattg ctgcatcccc acggccaggg
     7021 tgtagctgtt tacgtcacgc agtaacagcg acgcgaccgg aacttcagta atggcagcga
     7081 taaacgacag gatgaatacc accgccagaa tcggtactga cagcggcaac aggacaaggc
     7141 ggaaggcctg ccacggtgtc gcaccatcca gcgcagccgc ttcttccagc gaactgtcga
     7201 tggtttcgaa atagcctttg atggtccaga cgtgcagcgc aatcccaccc agatacgcga
     7261 aaattacgcc gccgtgagta ttcaggccaa tgaatggaat gtactcaccc agacgatcaa
     7321 acaacgcata caacgcgacc agtgaaagta ctgccgggaa catctggaaa atcagcattc
     7381 ctttcagcag cgtcgctttg cctggaaagc gcatacgggc gaaagcgtag gcgcaggtgg
     7441 tggagagcgc cacaatgcca atcgcggaaa tcccggcgac ctttaccgag ttccacagcc
     7501 acagcagcac cgggaatggc ggtggcgtaa tgcgaccatc agcctgttcc acgctaaaac
     7561 ctaatgccag tttccagtga tcccaggaga tttgctccgg gatcaggctg ccggtcgcaa
     7621 agttcccctg gcgcagtgag atagcgacga ccatcagcag cgggaacata atcgccgcga
     7681 taaaaagtag cagtagcagg tgagtaataa acaaacgtgc tttttgcgat ttcggttgga
     7741 ccattgccat ttttgttatc tcccttaatc aaacttcatt cgcgtggctt tcaggttcac
     7801 tatcgccagc gcacccacca gcaggaagat cagcgtggca attgctgctg ccagaccgaa
     7861 gtcctgaccc ccgccgcctt caaaagcgat gcggtaggtg tagttaacaa gcaggtcggt
     7921 ataaccggct ggcgtggtcg tgccaagacg atccgggccg ccgttggtta acagttgaat
     7981 cagcacgaag ttgttaaagt taaaggcgaa gctggcgatc atcagcggcg tcagcggttt
     8041 aatcagcagc ggcagcgtaa tcttaaagaa gttctggaac ggacctgcgc catccattgc
     8101 tgaggcttca tacaaatcgt ccggaatcgc tttcagcaag cccatgcaga ggatcatcat
     8161 gtacggatag cccagccagg tattgacgat aatcagcatc gttcgggcgg tggtcggatc
     8221 gctgaaccag gcgggcttca cgccaaacag cgcgctcaac atcatgttga tctcaccgaa
     8281 gctctggtta aacaaccctt tgaaaatcaa gattgaaatg aacgatggca ccgcgtaggg
     8341 cagaatcagc aggacgcgat agaccgcttt gccgcgcaac gcttcccact gcaccagaca
     8401 cgccagaacc atgccgactg ccaccgttaa aaagacagtg atcagcgaga acaccacggt
     8461 ccagacgaaa atggcgagga acggtttctg aatgccttcg tcggtgaaga cgcgagtaaa
     8521 gtttttccag ccggtggtca cggtgtaacc ggggcttagc ttttcatcac cccagttgcc
     8581 gtcggcggta atggactggt aaaagccaat ttggttattc ggacgatatt tcacgccgct
     8641 ctgattattc gtcaacgtgc cgtcaccgtc gagtgtgtag agcggctgcg tgccagaaaa
     8701 ctggcgcagg gagctcatca tcactttgtt gccatccggc agaatggcgg taatgtcact
     8761 cagcgcctga cgattctggg taatcacgcg tagatttgcg cgttcgcctt cgggctgggc
     8821 ggtcgtttct ttcagttgca gtttttgctc tctgccaaat ttaaaagcgt cggagaggta
     8881 atttttgccg gtttcgccgt cgctgagcgc cagttgccac tcatcgcccg ccgggtaaag
     8941 accaaagtta taggttttgc ctgcttgcca ggagcgatct aacaacactt cctgtgcacg
     9001 ttcaaaagtc agctggttag tgctgctgta gttggtgaag gcaatggcga tggtgcagac
     9061 cagagggaag aggacgaata atcccattcc agccattccc gggtaaacat agcgccaggc
     9121 gtaggcttta cgattggcga aaatatacag ccccgctgaa ctcaatatca gcgtggtaat
     9181 ggcgaacagg tattcccctt gtgcgtacat taaaacaaca aggtaaccca ccagcaggcc
     9241 gagcagacct aacactgacc atttcagcgc gtcgctttgc caccaatgtt tctttttaat
     9301 gacatccatg gggttcttcc tcattccagg acggataagg ctttcatgcc ttatccgaca
     9361 acaactgcct gatgcgacgc tgacgcgtct tatcaggcct acatacgttt cggttttgta
     9421 ggccggacaa ggcgttcacg ccgcatccgg catttcacag cattacttgg tgatacgagt
     9481 ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc
     9541 agtacgcacg gcataccaga aagcggacat ctgcgggatg ttcggcatga tttcaccttt
     9601 ctgggcgttt tccatggtgg ctgcaatacg tggatctttc gccaactctt cctcgtaaga
     9661 cttcagcgct acggcaccca gcggtttgtc tttattaacc gcttccagac cttcgtcagt
     9721 cagcagatag ttttcgagga actctttcgc cagctctttg ttcggactgg cggcgttaat
     9781 accggcgctc agcacgccaa cgaacggttt agatggttga cccttgaagg tcggcagtac
     9841 cgttacacca taattcactt tgctggtgtc gatgttggac catgcccacg ggccgttgat
     9901 agtcatcgct gtttcgcctt tattaaaggc agcttctgcg atggagtaat cggtgtctgc
     9961 attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc agacccgctt tcgcgccagc
    10021 gttatccacg cccacgtctt taatgtcgta cttgccgttt tcatacttga acgcataacc
    10081 cccgtcagca gcaatcagcg gccaggtgaa gtacggttct tgcaggttga acatcagcgc
    10141 gctcttacct ttcgctttca gttctttatc cagcgccggg atctcttccc aggtttttgg
    10201 cgggttcggt agcagatctt tgttataaat cagcgataac gcttcaacag cgatcgggta
    10261 agcaatcagc ttgccgttgt aacgtacggc atcccaggta aacggataca gcttgtcctg
    10321 gaacgctttg tccggggtga tttcagccaa caggccagat tgagcgtagc caccaaagcg
    10381 gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt gccgcaacct gagggaattt
    10441 ctcttccagt ttatccggat gctcaacggt gactttaatt ccggtatctt tctcgaattt
    10501 cttaccgact tcagcgagac cgttatagcc tttatcgccg ttaatccaga ttaccagttt
    10561 accttcttcg attttggcga gagccgaggc ggaaaacatc atcgtcgtta atgcggataa
    10621 tgcgaggatg cgtgcacctg tttttatttt cataatctat ggtccttgtt ggtgaagtgc
    10681 tcgtgaaaac acctaaacgg actctagttt ctttatacgg caacctcttt ccatcctcct
    10741 tgcccctacg ccccaccgtt gctttgtgtg atctctgtta cagaattggc agtaatgtgg
    10801 agatgcgcac ataaaatcgc cacgattttt gcaagcaaca tcacgaaatt ccttacatga
    10861 cctcggttta gttcacagaa gccgcgttct catcctcccg cctcctcccc cataaaaaag
    10921 ctagggggtg gaggatttaa gccatctcct gatgacgcat agtcagccca tcatgaatgt
    10981 tgctgtcgat gacaggttgt tacaaaggga gaagggcatg gcgagcgtac agctgcaaaa
    11041 tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat atcaatctcg atatccatga
    11101 aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt aaatcgactt tactgcgcat
    11161 gattgccggg cttgagacga tcaccagcgg cgacctgttc atcggtgaga aacggatgaa
    11221 tgacactccg ccagcagaac gtggcgttgg tatggtgttt cagtcctacg cgctctatcc
    11281 ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa ctggcaggcg caaaaaaaga
    11341 ggtgattaac caacgcgtca accaggtggc ggaagtgcta caactggcgc atttgctgga
    11401 tcgcaaaccg aaagcgctct ccggtggtca gcgtcagcgt gtggcgattg gccgtacgct
    11461 ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc tccaacctcg atgctgcact
    11521 gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa cgcctgggcc gcacaatgat
    11581 ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga
    11641 cgccggtcgc gtggcgcagg ttgggaaacc gctggagctg taccactatc cggcagaccg
    11701 ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc
    11761 caccgcaatc gatcaagtgc aggtggagct gccgatgcca aatcgtcagc aagtctggct
    11821 gccagttgaa agccgtgatg tccaggttgg agccaatatg tcgctgggta ttcgcccgga
    11881 acatctactg ccgagtgata tcgctgacgt catccttgag ggtgaagttc aggtcgtcga
    11941 gcaactcggc aacgaaaccc aaatccatat ccagatccct tccattcgtc aaaacctggt
    12001 gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc acattcgcta tcggcctgcc
    12061 gccagagcgt tgccatctgt tccgtgagga tggcactgca tgtcgtcgac tgcataagga
    12121 gccgggcgtt taagcacccc acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag
    12181 aaaagcaatg actcaggaga tagaatgatg attactctgc gcaaacttcc tctggcggtt
    12241 gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg ttgatttcca cggctatgca
    12301 cgttccggta ttggctggac aggtagcggc ggtgaacaac agtgtttcca gactaccggt
    12361 gctcaaagta aataccgtct tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt
    12421 caggaagtgt ggaaagaggg cgataagagc ttctatttcg acactaacgt ggcctattcc
    12481 gtcgcgcaac agaatgactg ggaagctacc gacccggcct tccgtgaagc aaacgtgcag
    12541 ggtaaaaacc tgatcgaatg gctgccaggc tccaccatct gggcaggtaa gcgcttctac
    12601 caacgtcatg acgttcatat gatcgacttc tactactggg atatttctgg tcctggtgcc
    12661 ggtctggaaa acatcgatgt tggcttcggt aaactctctc tggcagcaac ccgctcctct
    12721 gaagcaggtg gttcttcctc tttcgccagc aacaatattt atgactatac caacgaaacc
    12781 gcgaacgacg ttttcgatgt gcgtttagcg cagatggaag tcaacccggg cggcacatta
    12841 gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata actatcgtct ggttgatggc
    12901 gcatcgaaag acggctggtt attcactgct gaacatactc agagtgtcct gaagggcttt
    12961 aacaagtttg ttgttcagta cgctactgac tcgatgacct cgcagggtaa aggtctgtcg
    13021 cagggttctg gcgttgcatt tgataacgaa aaatttgcct acaatatcaa caacaacggt
    13081 cacatgctgc gtatcctcga ccacggtgcg atctccatgg gcgacaactg ggacatgatg
    13141 tacgtgggta tgtaccagga tatcaactgg gataacgaca acggcaccaa gtggtggacc
    13201 gtcggtattc gcccgatgta caagtggacg ccaatcatga gcaccgtgat ggaaatcggc
    13261 tacgacaacg tcgaatccca gcgcaccggc gacaagaaca atcagtacaa aattaccctc
    13321 gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc cggctattcg tgtcttcgca
    13381 acctacgcca agtgggatga gaaatggggt tacgactaca acggcgatag caaggttaac
    13441 ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac
    13501 agcgacgagt ggaccttcgg tgcccagatg gaaatctggt ggtaatagca aaacctgggc
    13561 cggataaggc gtttacgccg catccggcat aaaacaggtt gtcattatct gaaaggggcg
    13621 aaagcccctc tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat
    13681 tttgaggtga aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg
    13741 ttactggcaa gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc
    13801 agcgacgcgc cagccattcc atctgccgcg ctgcaacaac tcacctggac accggtcgat
    13861 caatctaaaa cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc
    13921 atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg
    13981 ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat
    14041 cagaacatga ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc
    14101 gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa
    14161 aaactttatg ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc
    14221 gacccggcta aagcctatgc caaaggcgtt ggtaactcga tcccggatat tcccgatccg
    14281 gttgctcgtc ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc
    14341 agcgtgttgg tcgggccttt atttggttct tccgctccag ctccggttac ggtaggcaac
    14401 acggcggcac cagctgtggc tgcacccgct ccggtgccgg tgaagaaaag cgagccgatg
    14461 ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat
    14521 gttgataagg cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt
    14581 tccaccttta tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg
    14641 caacaactgg tgcgtctcct ggcgcacctt tttttatgct tcattccagg gatatgagcg
    14701 attttttata gtaactcact tcttcttcac taagaatatc cattatctca atgtattatc
    14761 agagattctt ttcctttcgc cggtagtgtc tggacgctca ggctactttt tccagttatt
    14821 tcatttctgt aatgcagagg ttttatgtta ggtcatatct caaagtttga cggcaataac
    14881 tctt
//
LOCUS       DAZFTN010000112        14754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFTN010000112 DAZFTN010000000
VERSION     DAZFTN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..273
                     /locus_tag="ACHKV2_003903"
     CDS             <1..273
                     /locus_tag="ACHKV2_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941700.1"
                     /translation="KDQYKLTKNDYLKKVPLTAQLIAKYKMDDQLDQLLVTREIQDEI
                     KSKIQDKIDELSKNLFNTMTETIENNFDDIFRQQSENMSNYYEFVD"
     gene            complement(451..966)
                     /gene="pgpA"
                     /locus_tag="ACHKV2_003904"
     CDS             complement(451..966)
                     /gene="pgpA"
                     /locus_tag="ACHKV2_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO6941701.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(944..1921)
                     /gene="thiL"
                     /locus_tag="ACHKV2_003905"
     CDS             complement(944..1921)
                     /gene="thiL"
                     /locus_tag="ACHKV2_003905"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO6941702.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1999..2418)
                     /gene="nusB"
                     /locus_tag="ACHKV2_003906"
     CDS             complement(1999..2418)
                     /gene="nusB"
                     /locus_tag="ACHKV2_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO6941703.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2438..2908)
                     /gene="ribE"
                     /locus_tag="ACHKV2_003907"
     CDS             complement(2438..2908)
                     /gene="ribE"
                     /locus_tag="ACHKV2_003907"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO6941704.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2997..4100)
                     /gene="ribD"
                     /locus_tag="ACHKV2_003908"
     CDS             complement(2997..4100)
                     /gene="ribD"
                     /locus_tag="ACHKV2_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO6941705.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4104..4553)
                     /gene="nrdR"
                     /locus_tag="ACHKV2_003909"
     CDS             complement(4104..4553)
                     /gene="nrdR"
                     /locus_tag="ACHKV2_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO6941706.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4704..5243
                     /locus_tag="ACHKV2_003910"
     CDS             4704..5243
                     /locus_tag="ACHKV2_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6941707.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5542..6426
                     /gene="tsx"
                     /locus_tag="ACHKV2_003911"
     CDS             5542..6426
                     /gene="tsx"
                     /locus_tag="ACHKV2_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6941708.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6603..6950)
                     /gene="yajD"
                     /locus_tag="ACHKV2_003912"
     CDS             complement(6603..6950)
                     /gene="yajD"
                     /locus_tag="ACHKV2_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO6941709.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7038..7319)
                     /locus_tag="ACHKV2_003913"
     CDS             complement(7038..7319)
                     /locus_tag="ACHKV2_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6941710.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7312..7554)
                     /locus_tag="ACHKV2_003914"
     CDS             complement(7312..7554)
                     /locus_tag="ACHKV2_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6941711.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7706..8677)
                     /gene="secF"
                     /locus_tag="ACHKV2_003915"
     CDS             complement(7706..8677)
                     /gene="secF"
                     /locus_tag="ACHKV2_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO6941712.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8688..10535)
                     /gene="secD"
                     /locus_tag="ACHKV2_003916"
     CDS             complement(8688..10535)
                     /gene="secD"
                     /locus_tag="ACHKV2_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO6941713.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10563..10895)
                     /gene="yajC"
                     /locus_tag="ACHKV2_003917"
     CDS             complement(10563..10895)
                     /gene="yajC"
                     /locus_tag="ACHKV2_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO6941714.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10918..12045)
                     /gene="tgt"
                     /locus_tag="ACHKV2_003918"
     CDS             complement(10918..12045)
                     /gene="tgt"
                     /locus_tag="ACHKV2_003918"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO6941715.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(12100..13170)
                     /gene="queA"
                     /locus_tag="ACHKV2_003919"
     CDS             complement(12100..13170)
                     /gene="queA"
                     /locus_tag="ACHKV2_003919"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO6941716.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13263..13844
                     /gene="acpH"
                     /locus_tag="ACHKV2_003920"
     CDS             13263..13844
                     /gene="acpH"
                     /locus_tag="ACHKV2_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO6941717.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13865..>14754)
                     /locus_tag="ACHKV2_003921"
     CDS             complement(13865..>14754)
                     /locus_tag="ACHKV2_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941718.1"
                     /translation="YSKRTKQLEKKIADAGFITRKVKAENFNVKEFKDDIASMLCEQP
                     PMRTHQISSSGYVSADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIA
                     KKTAEIIAKQNAKSIEEKNIEKNKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLR
                     PFFTSDHIYCLFDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTG
                     GVLHRHISVKSEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHS
                     SECFSYRCYAAPLDANAKK"
BASE COUNT         3704 a         3912 c         3496 g         3642 t
ORIGIN      
        1 aaagaccagt ataaactaac aaaaaatgat tatttgaaga aagtaccttt aactgcacaa
       61 ttaattgcaa aatacaaaat ggatgatcaa ttggaccaac ttttggttac ccgtgaaatt
      121 caagatgaaa taaaatcaaa gattcaagac aaaatagatg aactttctaa aaatttattc
      181 aacactatga ccgaaaccat tgagaataat tttgatgata tttttcgtca acagtcagag
      241 aatatgagca attattatga gttcgtcgac tgacgatatt tcagtgtcat tgaattatga
      301 gtgacaaaga tacaccttga aatatcgccg catagtatat tcggcgatgt cgggttttac
      361 tttaacagag tgccccaaaa ttggggcact gttcatttat aaatcaaacc gggtggtctg
      421 gaatcgccta cacctcccgg tcaatggcat ttaaagaata cccagcggcc agtgatgacc
      481 gataaagtac aggatgcctg cggaaatcac cccggcgaca atatcatcga tcatgatccc
      541 catgccgcca tgtacattgc gatcaaacca gcggatcggc cacggcttcc acatatccag
      601 aatacggaaa atcacaaacc cggcggcgac ccactgccag tcattggtcg gcagcgccat
      661 cagggtgatc cacatgccaa taaactcatc ccatacgatg ctaccgtgat cgtgcacccc
      721 catgtctttc gccgtttgat gacaaagata gacgccgata cagatcccca acatcaccac
      781 cagcgagtag agctgccagg gcaaaaaggt catcagatac cagaacggaa tcgccgccag
      841 cgatcccatc gtgccaggaa cgattgggct taatccactt ccgaatccga cagcaagcag
      901 atgccacgga ttactcatct tcaggcgact tttcgcgaca tctttatggc gtggcaaaat
      961 ggtcatatcc tttccagtct aacgtgacag gttcgccgtc acgaataaaa caaagccctt
     1021 cgatatcggc ggtcatttgc ccgatacagg taaacggtac gcccagatga ccgagagcca
     1081 catccagcgc gccacggttc agttccggca ccgtgaaaca caactcgtaa tcttcaccgc
     1141 cagagagcgc ccagcgcagc gcctgttccg gtacaacatg gcgagaaagc gcatcagaaa
     1201 acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg atatgcccga
     1261 gatcggaaat cagaccgtca gagagatcga tagctgaatt tgccagatcg cgcagtgcct
     1321 gcccctgtaa aatacgcggc gatggacgga gatgacgttt gatcaagtag tccgcatctt
     1381 tagcatcggc aacctgcaaa cggttttgca aaatcgccag cccggcggcg ctatcgcccg
     1441 gtgtaccggt cacatagatc cagtcacccg gtttcgcccc agagcgcgtt aaggctcgtc
     1501 ccatcggaac aaagccgtgg atacccaacg tcattgataa tggcccacgc gtggtatcgc
     1561 cgccaatgag ttgcatatcg taataattga gaagatcaaa caaactgtcg ctgaaggact
     1621 caagccacgc ttcgtctacg tccggtaagg ttaatgccag cgtcagccag gccggatcgg
     1681 cccccatcgc tgccagatcg cttaggttca ccgccagtgc tttataagcc agatcagcag
     1741 gatcgatatc agggaggaaa tggttgcccg ccaccagcgt atcagtgctg atcgccaggg
     1801 tctgtttctc ggggatattg agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa
     1861 gacgagaact tcttacacgg tcaaaataac gggcaatcag ggagaactcg ccacatgcca
     1921 tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga tcgcggaaca gtcttccgtg
     1981 aatctaccgg cctggatatt actttttgtt agggcgaatc acaggtgctg ctttatcgag
     2041 tacgccgttg acgaacttat ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc
     2101 ttcgttaatg gccactttgt atggcacatc gctacgttta gacagttcgt acagcgcaat
     2161 gcgcagtact gctttttcta cctgtcccag ttcttccagc aggcgggaca ggtatggctt
     2221 catcagtccg tcgaggtatg cggtattagt cgccaccccg gccagcagct cacggaagta
     2281 caggacgtca acgtctttta catcctgttc agccaggaac tggtattcaa catcagcgat
     2341 gtcgttctgg gacaactgcc aggagtagag cgcctggacg gcacactcac gagcgcggcg
     2401 acgagcagca ggtttcacgg atttcccctt actaatttca ggccttgatg gctttcaata
     2461 cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca gctttggtgc
     2521 cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa
     2581 tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg acatattcaa
     2641 aatgggcagt gccaccacga ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg
     2701 ttttagccag tgcacccgcc gccagcggca gctcataggc accaggcacc caaacaacgg
     2761 taatgttttc atcttttacc tgaccgatac gtttcagtgc atcaattgca ccttccagca
     2821 ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg
     2881 tagcaacgtt agcttcaata atgttcattt tcttccttcg ggttcgagta tggccccgca
     2941 ggggggcgga ttttagcata atatttcgtg cgctgcttcc ctttcgagcc gggagatcat
     3001 gcacccacta aatgcaggca aacatccggg cctacatgac gtatctcttt gaatttaaat
     3061 tggggggcgt cagctaattt ctcaagccct ggcagtgtgc ataatccacg ggcgtcgctg
     3121 cctaatagtt taggtgcgat atagacaatc agctcatcga ctaaccccgc ctgcagcaat
     3181 gcgccagcga gcgttggccc cgcttccacc cagatgctgt taatctgctg tttacccagt
     3241 tgcatcatca gtacaaccag atccagatga cctttatgct ctggaatcag caaggtacgc
     3301 accgtttccg gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc
     3361 tgctgcacaa tgtgatgttc cggcgtcacg cgattttggc tatcaatcac aatacgtacc
     3421 ggctggcgga gatgatgttg cgggtagagc gcctgagttt gtacatccag ttcagaccaa
     3481 cgcaccgtta aggcaggatc atccgccagc accgtagcgc tgctggttaa aatggcatga
     3541 ctttgtgcgc gcagtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt
     3601 tcgccgctcg ccatcgccgt gcggccatca agcgatgcgc caagttttaa ctgaatataa
     3661 ggaaagccgg tgcgcatccg cttgagaaag cctttattca attgctcggc ttcactcatc
     3721 atcaggccgt ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg
     3781 acctgcgggt tcgggtcctg catcgcagcg accacgcgcg ctacgccagc ggcgatgagt
     3841 gcgtcacagc acggtggcgt acgaccatga tggctacagg gttcgagtgt gacataggcg
     3901 gtcgcacctt tggctttttc acccgccata cgcaacgcgt gtacttcggc atgtggttca
     3961 cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat ctttgacaat gacgcacccg
     4021 acattcgggt tggggtgggt ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc
     4081 atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc gccaaattct
     4141 ttgatatctt cgaaactgcg gtagacagag gcaaaacgga tataggcgac tttatcgagc
     4201 tttttcaatt gctccatcac cagattgcca atcatcttgc tcggcacttc gcgctcaccg
     4261 gtggcgcgca gctgcgattt aatatgattg attgccattt cgacgtcatc ggaactcacc
     4321 ggacgttttt ccagcgcccg cagcattccg ctacgtaatt tttcttcatt aaacggttca
     4381 cgcacgtcgt tgctttttac aacacgcggc ataaccagct ccgccacttc aaaggtggtg
     4441 aaacgttcat tacacaccag acactgccga cggcggcgta cggatgaacc ctcgcccacg
     4501 agacgagagt caattacctt agtgtccacg gcgaaacaga atgggcaatg catacggcgt
     4561 cctgaccagg tggttaacgg aaatctattt taccctgaac tgacgataca acaaaggcgc
     4621 aacgcttttg agataaagga tcgatgcttt cgctgctttt accctctacc attaggctaa
     4681 ttgcgacgag aaagggacga cctatgaata ttaacgtttt tcgactgatc ctgctgggaa
     4741 gcctattcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg
     4801 tcagcacgct gaaccaggag atgacgcaac tcaatcaaga aacagtcaaa atcactcagc
     4861 aaaacaggct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga gcgaaaacac
     4921 cggcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg
     4981 atacagatgg caccaccctg acactacgta ttcagggtga gtcgaatgat cccttgcccg
     5041 cattcagcgg aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa
     5101 tcaatgtaca gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg
     5161 atattccgtt acagttaaag gggatttctg tggatcagtt aggctttgta cgtatccacg
     5221 acattcaacc tgttatgcag taaacttata gcggggcgat tttgtgcgtc ccgcaacatc
     5281 cttccccatc attttgttac tccgcttaca taacccagat tgatagtaaa agtttgcaac
     5341 aagggcgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg caatcgatta
     5401 cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg
     5461 ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga
     5521 ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg
     5581 ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac
     5641 tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg accgcagatc
     5701 cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt cgacttctat
     5761 ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgcaaaagg tatctggaac
     5821 cacggttctc ctctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat
     5881 actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac
     5941 atgggtcgta ataaagatgg tcgccagagc acctggtaca tgggtctggg taccgatatc
     6001 gacactggcc tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac
     6061 tatggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg
     6121 ccgattaccg atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg
     6181 ggttccgatt taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtactaat
     6241 aactctatcg cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc
     6301 gtagctcgtt actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc
     6361 aacggcaact tcaacgttcg ctctaccggc tggggtggtt acctggtagt aggttacaac
     6421 ttctgattat gaaaatgccg ggatttattc ccggcatttc agattgttaa caacgtacgc
     6481 tttgttcatg ccggatgcgg cgtaaacgct ttgtccggcc cacaaaagca cgtaaattca
     6541 atatattgct gaaataacgt aggcctgata agcgtagcgc atcaggcaat tttacgtttt
     6601 aatcacttct tcttgttcat catcgctttc aggtcagcga atgggttgta cttcgcttca
     6661 ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttcg
     6721 gtatatttcg aatgctcatg atcgtggcaa tagagacaaa gcaattccca gttactgcca
     6781 tcttccgggt tattggtatg gtcgtgatca atgtggtgaa cggtaagttc acgcaggttg
     6841 gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt taatgctttt
     6901 tcgcgatagc cgctttctaa ccgcgcatag ttttttggga tgatagccat gaaaagttac
     6961 ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatcctga
     7021 gtcaagaaat tctcacatta ccagtgacgg cgaggttcca tcaactgatg aagaatgcgc
     7081 gcaatgagaa tgtcatcgac tcttataacg taataaacag tgtgaaacct aaatgcgatc
     7141 ttctttacct ctggaatcgc atcatagtct tgccctatct cgggcatttg agagagtgta
     7201 tccagaaaag tgccgagaga gtcggtatag cgatccgcct gctcatcacc aaactttccc
     7261 agcgtgtagt catagatccc tctgaaatct tcaacagctt ttccgctaag cttatacacg
     7321 atgtttttgc ttcctttctg ctgcgatatc acgaagcgtc atcgaacttt caccgctctc
     7381 aagaccttca ataacagcat ttctgacagc ttcatccctg gattcttgtt gttccaatag
     7441 acgaagtgcc gaacgcatta cttcactggc cgagccataa cgaccagact ggaccatccg
     7501 ggtaataaaa ctatctaatt gttcgccaac gcttactgag gtaattttgc gcataagcga
     7561 tctccacgta ttaactatta atacaaagta tatgtcagaa cgctttctac aaccaaaccg
     7621 tattcagttg cctgtgaaac aggcaaaaaa aaaccgcagc gcaaatacgc cacggttttt
     7681 ttaacagaac ggatttaaaa caccattacg gcagaattga cggctgatcc gccccttctt
     7741 tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccaacgcag
     7801 atgccacata gatggaagat gcagtaccga tggaaacacc gataagcatg gtcagcgaga
     7861 agccttccag taccggacca ccgaagagat acagcatcag gataaccatc aaggtagtac
     7921 cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag
     7981 gcgtaccgcg acggatcttg cggaagtttt cacgaattcg gtccgatacc acgatactgt
     8041 cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg tcaatctcga
     8101 tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc gcaataacca
     8161 cccctgccgc cagtcgccac tcaaagcgga aacctacgta cacgaggata gacagcagcg
     8221 ctgccatcaa cgccatcgca ccggtttgcg caaggtctgc ccccacgctc ggaccgacga
     8281 actcaatacg cttcactgct gcattctgat tggtggattc gttaatcacc ttcagaacct
     8341 ggctgcccag cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt
     8401 cgtggctgct accaaagttt tgcagcatcg gctcttcaaa accggctttt tgcaatgcat
     8461 cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcgataacc gtaccaccgg
     8521 tgaaatccag cccccagtta aagccgcgca cgcccataat aacgatagca gcgattaaca
     8581 gcagaccaga gatgccaaaa gcccagtagt cccagcgcat aaagtcatag actttacggc
     8641 cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc
     8701 ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg
     8761 gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg
     8821 gtacccactg cgtacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagata
     8881 gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg tccgttgctc
     8941 aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt
     9001 aagacgatac ccgcgatacc tggcatactc agtgttgcgc ctggcaacag cgacataata
     9061 ccgacgatca agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta
     9121 tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc
     9181 tgctcaatgt tctgcatacc cagggtcggg ccaattgtac gttcttcaac aatctgaatc
     9241 ggcgcgatca acgcaccggc acgcagcagc agtgacagct gacgggcttc gttcgggttg
     9301 ttgatgccgg tgatacggaa gctgttaccc agacgagact ggatgttggc gatgttaatc
     9361 acctcttcct gtttcaccag aaccgcacga ccatttgcat ctttcttacc gctgtctttg
     9421 tactccacaa acagggttgc catcggtttg ccgatgttgt ccttggtgaa gttagacata
     9481 atgttgccac cagcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg
     9541 ctggaagtgg agtcggtgat atggtcaccg gtaagaatta cgcgtttgta cagcacaacc
     9601 ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc ggatgccgca
     9661 gcggcctgat caacgttggt gtttaccaga cggaattcca gcgttgccgt cgcacccaga
     9721 atctctttcg cacgcgcagt gtcctgaata cctggcagtt caacaacgat acggtcagca
     9781 ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata
     9841 ttgatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc
     9901 gcacgcagct ggttgctgcc ctgactgcta atcaccaggt ccggatgacg cttgctcaga
     9961 tacgcaatgg cttcatcacg agctttagca tcgcggaaag tgatgctcag gccgtagtta
    10021 ttttctttac gaacggtggt atacgggatg cctttttcgc gcaggtcgct gcgcaggcta
    10081 tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag
    10141 tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcgtgaat agctgccagc
    10201 cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc
    10261 attaatgctt cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc aagaatagcg
    10321 ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc
    10381 tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga
    10441 tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc
    10501 atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt
    10561 aattacagcg ccttcatggt gcctttcggc aggacggcag ctacgaagtc acgtttaata
    10621 actacttcag tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc tactttggtt
    10681 acgcgaccaa ccaggccacc gttcgtcaga acttcatcac ctttggcaat ggagtccatc
    10741 agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc
    10801 agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc
    10861 gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta
    10921 atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct
    10981 ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg
    11041 aaggttatga atggtattga gtcgcgcgcc taatatttcg ttgcaacggt caagatgatg
    11101 caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc
    11161 agtatcgctc ttatacttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg
    11221 accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac cacgacgtac
    11281 gccttcaacc aggtcttctg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg
    11341 aatttgcggg catacatgct ccagaatgcg gtgcatatct gctttcggct cacccacagc
    11401 cagaccgccg acagcgtagc catcaaaacc gatatctacc agacctttaa cagaaatatc
    11461 acgtaaatct tcgtaaacgc tgccctgaat gataccaaac agcgcatttt tgtttccgag
    11521 actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tggagcgttt
    11581 tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga
    11641 accaagatcg tactgaatct ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt
    11701 gatcgggtta cggaagtgca cgccctgttc ggtgatttta cgaatatcgc caaggctgaa
    11761 gacttggaag ccgccggagt cggtgaggat cggtcccttc cactgcataa aatcgtgcag
    11821 atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga aggtgttgcc
    11881 gaggataatt tgcgcgccag tggcttcaac ttcttccggc gtcatccctt ttacggtgcc
    11941 gtaggtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg
    12001 gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg
    12061 tcagaaaaac agtccaacgt ttaaaccagc gccgcggaat tactccccga cgcgctcatt
    12121 aattgcctgc ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt
    12181 ctcttctacc gccgctttat aggcattcat tgtgtgttga taaccggcaa aggccgaaac
    12241 cagcataatc agcgtcgatt ccggaaggtg gaagtttgtc accagcgcat cgaccacttt
    12301 gtactggaag cccggataga taaagatttg ggtatcgtca aagaacggtt cgatgagatc
    12361 gtttttcgcc gcctgagccg cgctttccag cgaacgtact gaggtggtgc caaccgcaat
    12421 cacccggtta ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggtacttc
    12481 agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg acgcgtaccg gctggaaggt
    12541 gcccgcacca acgtgcaggg tgacgaacgc catctccacg cctttggcgc gtaatttttc
    12601 cagcaaaggt tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc
    12661 gctataaacg gtttgataaa gttcgcggtc agcgtcttcg tccggacggt cgatatacgg
    12721 cggcagcggc atatggccga tgctgttgag aatatccagc accgagcgtt catcattaaa
    12781 ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac tttcgtcatc
    12841 gcccagcagc agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat
    12901 gcgtttgtcg tcgagcatcc gttcaaccag cacttcaatc ttgccgccgc tggctttacg
    12961 cccaaacagg cgcgccggga tcacccgggt attattaaaa accagaagat cgccggggtt
    13021 gagcttatcg agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag
    13081 cgacagtaaa cgacagctac tgcgttcagg catcggatag tgggcaatca gggattcggg
    13141 caattcaaag gagaaatcgg taacgcgcat gacactgact cagactaaaa taagaggcgg
    13201 gtagtctagt gccggggcgc tctccctgca acctttaccc ccctttggat agaatataat
    13261 cgatgaattt tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttccggca
    13321 atttgctggc tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg
    13381 ctggcattca tatgcatcga cgtatcgacg tattgactga caatctgccg gaagtccgcg
    13441 aagcacggga gtggtttcgt agtgaaactc gccgcgttgc gcctattacg ctggatgtca
    13501 tgtgggatca ctttctttcc cgtcactggt cgcagctgtc gccggacttt ccgctacagg
    13561 aatttgtttg ttatgcccgc gagcaagtga tgacaatttt gccggactca ccgccacgct
    13621 ttatcaacct gaacaactac ttatggtcag agcagtggct ggtgcgctat cgcgatatgg
    13681 atttcatcca gaacgtgtta aacggcatgg caagccgtcg cccacgtctg gatgccctgc
    13741 gtgactcctg gtacgattta gacgcccatt atgccgccct cgaaacccgc ttctggcagt
    13801 tttatccacg gatgatggcg caggcgtcac gcaaggcgct ctgaaacttc aaactccggc
    13861 gtcactattt tttagcattc gcatccagcg gggcggcata acaacggtaa gaaaaacatt
    13921 cgctggaatg attggcgata tctttaaagt tgctttcagc aaccccctcg aacatgagta
    13981 ccgttctgga caactctccg ttttcattta tatgtggaaa atcaaattgg ctactgaatc
    14041 gccatacatc tgtcttttct ggctcgctct tcacagagat atgtcgatgg aggacccctc
    14101 ctgtaaacgt gcgccagaac aattgttgat caaaatcttg taatatcgca gacttggatg
    14161 ctatttgacg tagtaaacct gcataccgtg gactgccatt acgagcaaca ttatgcaaac
    14221 cctgactaaa agcatcaaac aaacaataga tgtgatcact agtgaaaaaa ggtcttaatt
    14281 ttttttgttc ttcatcgctg taatccagga gcatctcatt gatttcttcc ggtgagaatt
    14341 ttaagacaaa attatcctca gaatttgttt gtttcttgtt tttttcaata ttcttctcct
    14401 caatgctctt cgcattttgt ttcgcaatta tttccgcagt ttttttcgca atttcttccg
    14461 caattttttc ccgagttatt gattttagac taacaccatc gatgtcaacc ggtgttgttt
    14521 tcagatctct ctgcgcgtct tccaaatttg tcttatagag tttgtgcagt ttatcggcag
    14581 atacgtatcc ggatgaagaa atctggtgtg tacgcatggg gggctgttca caaagcatcg
    14641 aggctatatc gtctttaaac tcttttacat taaagttctc ggctttaacc ttccttgtta
    14701 taaaccccgc atcggcaatt ttcttctcta attgttttgt acgtttagaa taat
//
LOCUS       DAZFTN010000113        14706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFTN010000113 DAZFTN010000000
VERSION     DAZFTN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..205
                     /locus_tag="ACHKV2_003922"
     CDS             <1..205
                     /locus_tag="ACHKV2_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941719.1"
                     /translation="EKNIFTFDNSKFVDPKEKEGLMVQHSNGQLVITGKYCPEGVQTA
                     FTQEQYDKLIRYINIFFTFPKLE"
     gene            complement(429..1505)
                     /gene="glpQ"
                     /locus_tag="ACHKV2_003923"
     CDS             complement(429..1505)
                     /gene="glpQ"
                     /locus_tag="ACHKV2_003923"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6941720.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1510..2868)
                     /gene="glpT"
                     /locus_tag="ACHKV2_003924"
     CDS             complement(1510..2868)
                     /gene="glpT"
                     /locus_tag="ACHKV2_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO6941721.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            3141..4769
                     /gene="glpA"
                     /locus_tag="ACHKV2_003925"
     CDS             3141..4769
                     /gene="glpA"
                     /locus_tag="ACHKV2_003925"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO6941722.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4759..6018
                     /gene="glpB"
                     /locus_tag="ACHKV2_003926"
     CDS             4759..6018
                     /gene="glpB"
                     /locus_tag="ACHKV2_003926"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO6941723.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            6015..7205
                     /gene="glpC"
                     /locus_tag="ACHKV2_003927"
     CDS             6015..7205
                     /gene="glpC"
                     /locus_tag="ACHKV2_003927"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO6941724.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7398..8324
                     /gene="rpnE"
                     /locus_tag="ACHKV2_003928"
     CDS             7398..8324
                     /gene="rpnE"
                     /locus_tag="ACHKV2_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO6941725.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(8365..9159)
                     /gene="yfaU"
                     /locus_tag="ACHKV2_003929"
                     /pseudo
     CDS             complement(8365..9159)
                     /gene="yfaU"
                     /locus_tag="ACHKV2_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
     gene            complement(9185..10475)
                     /gene="yfaV"
                     /locus_tag="ACHKV2_003930"
                     /pseudo
     CDS             complement(9185..10475)
                     /gene="yfaV"
                     /locus_tag="ACHKV2_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10532..11737)
                     /gene="rhmD"
                     /locus_tag="ACHKV2_003931"
     CDS             complement(10532..11737)
                     /gene="rhmD"
                     /locus_tag="ACHKV2_003931"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO6941726.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(11752..12534)
                     /gene="yfaX"
                     /locus_tag="ACHKV2_003932"
     CDS             complement(11752..12534)
                     /gene="yfaX"
                     /locus_tag="ACHKV2_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6941727.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12754..13956)
                     /gene="yfaY"
                     /locus_tag="ACHKV2_003933"
     CDS             complement(12754..13956)
                     /gene="yfaY"
                     /locus_tag="ACHKV2_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO6941728.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(14056..14598)
                     /gene="yfaZ"
                     /locus_tag="ACHKV2_003934"
     CDS             complement(14056..14598)
                     /gene="yfaZ"
                     /locus_tag="ACHKV2_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO6941729.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
BASE COUNT         3696 a         4020 c         3610 g         3380 t
ORIGIN      
        1 ggaaaaaaat atcttcacat ttgataattc taagtttgta gatccaaagg aaaaagaagg
       61 tttaatggta caacacagca atgggcaact ggttattact ggtaaatatt gccctgaagg
      121 tgtgcaaaca gcattcaccc aagaacaata tgataaatta ataagataca ttaatatttt
      181 ctttaccttt ccgaaattag aataatactt tacagtataa actgcattac aatttattat
      241 tgagacctat tcatactgtt taaagaataa caatgcccag tgttagcttt tttaacgaat
      301 gagttttacg aaaattatgt tggcatcttt tttacaggac gcaccggtta atgaaaagaa
      361 taaccggtag tagcattatt tcgctttagc agaggatggc gcatgaacgc catcctgcag
      421 tagcagcatt actctttatt aaggaattta accgctttat cagggaaatc agtaaacaac
      481 ccatttacac ccgctttgtt atacagagca tcatataact gattcacatc agttgtgtat
      541 tcaggcagtt tatctgaccg cacggtataa ggatgcacta ccagcttgtt ctgctgagca
      601 tcttgcacca tgccagtgag tttgatatta cccggctgcg atgtctcctc aatcaacata
      661 tggtaatccg gaccaatacc atctgcatat tccgccacct gtttcatggc acccggctta
      721 aacatccagt cgtagttgta attaacccag cttccgtccg gctgtttctg ctgcgtttca
      781 ttccagtcgg tataggcaat cagctgtacc agattgagat ccatgcccat tttgggttcc
      841 agctcattct taatacgctt cagctcatca gcatcaaaac attgcaaata aactttatcg
      901 tctttaccgg tgtaaccata tttcttcagc acttccagcg tttttgccgc aatatccttc
      961 ccttcctgat gatggaacca cggcgctttg atttctggat agataccgat atttttcccg
     1021 gtagagtgat ttaacccctg aacaaattca atctcttctt caaaggtgtg cacccggaag
     1081 tcggacttac ccattgggaa acgccctgga taagtctgca cttttttacc gttttcaata
     1141 tcgaaacctt cggtaaattt caacgactta atttcatcca gcgtgaaatc tatcgcgtag
     1201 taacgaccgt ctttgcgcgc ccgatccggg aaacgatcgg caacatcagt aacacgatcg
     1261 aggtaatggt catgcagaac aaccagatgg tcgtctttgg tcatcaccaa atcctgttcc
     1321 agataatccg ctccctgcgc atacgccatc gcttttgctg gcagcgtatg ctccggcaaa
     1381 tatccactgg caccgcgatg ggcgatgact attttttcgt tgctgtccgc cgccattgca
     1441 ctgcttccca tgactatagt gctcatcatg atcgccatgc taaggttttt cagcgtcaat
     1501 ttcatgccat tagcctccgt tgcgtttttg cagtaattcc tgatggcgac gtttttcgcc
     1561 aatcatcaca acaatcaaca agataaccgc tagaatgctg ccgccaatca ttaccataaa
     1621 gccgccatcc cagccgaaga agtccacggt gtagccaaca atcgcgctcg ccgccaccga
     1681 accgcccagg taaccaaaca gaccggtaaa gcccgctgcc gttcctgccg cttttttcgg
     1741 tgccagttcc agcgcatgca gaccgatcag catcacaggg ccgtagatca ggaagccgat
     1801 aacaatcata caaatcatat cgacggttgg gttacctgcc gggttcatcc agtaaacgat
     1861 agtcgcgatg gtcaccagtg tcataaagaa aacgccggtt gccccacggt tgccacggaa
     1921 gactttatcc gacatccagc cgcacagcag ggtgcccgga atacccgcat actcatagaa
     1981 gaagtaggcc caggaggatt tatccagcgc gaaatgctta acctctttca gataagtcgg
     2041 tgaccagtcg aggatgccgt aacgcagcag ataaacgaac acgttggcga tggcgatata
     2101 ccacaacagt ttgttcggca gcacgtactg catgaagatt tgcttcgcgg tcagctcctg
     2161 ttccgctttt tcgttatagt cgtccggata atcatttttg tactcttcga tcggcggcaa
     2221 gccacaggat tgcggggtat cgcgcatcat cgcaaaggcg aataatgcca ccagaatggc
     2281 gcagaaagcg ggcatataga gcgccgcgtg ccagtcattg aaccaggcca tccccagcag
     2341 gaacagcagc ggcggaatac caccaccgac gttgtgcgca cagttccaca ctgacacaat
     2401 gccgccacgt tctttctgcg accaccagtg caccatagta cgaccgcatg gcggccaccc
     2461 catcccctgg aaccaaccgc agaggaacaa cagtacaaac atcaccgcaa tgctcgacgt
     2521 cgcccatggc acaaagccca taaacaacat cactgccgcc gccagaatca aacctgcggg
     2581 caggaaaacg cgcggattcg agcgatccga taccgaaccc atgatgaatt tcgaaaatcc
     2641 ataggcgatc gagatcccag aaagcgcaaa acctaaatca ccgcgtgaga atccctgctc
     2701 caccaggtaa ggcatagcga gcgcaaagtt cttacgaacc aaatagtaag cagcatagcc
     2761 aaagaatatc cccaggaaaa tttgccagcg caatcgacga taagtcggat cgatctccgc
     2821 ggcaggtaag cgcgctttgt gtggcgctgg tttaaaaata ctcaacattg aaagcctccg
     2881 tggcccgtgg tcttatttat gattaacagc ctgattcagt gagagaacct gccgtttctt
     2941 gagttgccgc gatgttaaga aaacattcat aaattaaatg tgaattgccg cacacattat
     3001 taaataagat ttacaaaatg ttcaaaatga cgcatgaaat cacgtctcac tttcgaatta
     3061 tgagcgaata tgcgcgaaat caaacaactc atgtttttac tatggctaaa tggtaaaaaa
     3121 cgaacttcag agggataaca atgaaaactc gcgactcgca atcaagtgac gtgattatca
     3181 ttggcggcgg cgcaacggga gccggaattg cccgcgactg tgccctgcgc gggctgcgcg
     3241 tgattttggt tgagcgccac gacatcgcaa ccggtgccac cgggcgtaac cacggcctgc
     3301 tgcacagcgg tgcgcgctat gcggtaaccg atgcggaatc ggcccgcgaa tgcattagtg
     3361 aaaaccagat cctgaaacgc attgcacgtc actgcgttga accaaccaac ggcctgttta
     3421 tcaccctgcc ggaagatgac ctctccttcc aggccacttt tattcgcgcc tgcgaagaag
     3481 cagggatcag cgcagaagct atagacccgc agcaagcgcg cattatcgaa cctgccgtta
     3541 acccggcact gattggcgcg gtgaaagttc cggatggcac cgttgatcca tttcgtctga
     3601 ccgcagcaaa catgctggat gccaaagaac acggtgctgt tatccttacc gctcatgaag
     3661 tcacggggct gattcgtgaa ggcgcgacgg tgtgcggtgt tcgtgtacgt aaccatctca
     3721 ccggcgaaac tcaggccctt catgcacctg tcgtggttaa tgccgctggg atctgggggc
     3781 aacacattgc cgaatatgcc gatctgcgca ttcgcatgtt cccggcgaaa ggatcgctgc
     3841 tgatcatgga tcaccgcatt aatcagcatg tgatcaaccg ctgccgtaaa ccttccgacg
     3901 ccgatattct ggtgcctggc gataccattt cgctgattgg caccacctct ttacgtattg
     3961 attacaacga gattgacgat aatcgagtga cggcagaaga ggttgatatt ctgctgcgtg
     4021 aaggggaaaa actggccccc gtgatggcga aaacgcgcat tttgcgggcc tattctggcg
     4081 tgcgcccgct ggttgccagc gatgacgacc cgagcggacg taacgtcagc cgtggcatcg
     4141 tgctgctcga ccatgctgaa cgcgatggtc tggacggatt tatcaccatc accggtggca
     4201 aactgatgac ctatcggctg atggctgaat gggctaccga cgcggtatgc cgcaaactgg
     4261 gcaacacgcg cccctgtacg accgccgatc tggcacttcc gggttcacag gaacccgctg
     4321 aagttacctt gcgtaaagtc atctccctgc ctgccccgct gcgcggttct gcggtttatc
     4381 gtcatggcga tcgcacgcct gcctggctga gcgaaggccg tctgcaccgt agcctggtat
     4441 gtgagtgcga agcggtaact gcgggtgaag tgcagtacgc ggtagaaaat ttaaacgtta
     4501 atagcctgct ggatttacgc cgtcgtaccc gtgtggggat gggtacctgc cagggcgaac
     4561 tctgcgcctg ccgcgctgct ggactgctgc aacgttttaa cgtcacgacg tccgcgcaat
     4621 ctatcgagca actttccacc ttccttaacg aacgctggaa aggcgtgcaa cccatcgcct
     4681 ggggagatgc actgcgcgaa agcgaattta cccgctgggt ttatcaggga ctgtgtggtc
     4741 tggagaagga gcagaaagat gcgctttgat actatcatta tgggcggcgg cctcgccgga
     4801 ttactctgtg gcctgcagct gcaaaaacac ggcctgcgct gtgccattgt cacgcgtggt
     4861 caaagcgcgc tgcatttctc atccggttcg ctggatttgc tgagccatct gccagatggt
     4921 caagctgtga cagacattca cagtggactg gaatctttgc gtcagcaggc acctgcacat
     4981 ccttactctc ttctcgggcc acaacgcgtg ctcgatctcg cttgccaggc gcaggcatta
     5041 atcgctgaaa gcggtgcgca attgcagggc agcgtagaac tgcctcacca gcggattacg
     5101 ccgctcggca ctctgcgctc tacctggcta agttcgccag aagtccccgt ctggccgctg
     5161 cccgcgaaga aaatatgtgt agtgggaatt agcggactga tggattttca ggcgcacctt
     5221 gcggcagctt cgttgcgtga actcgacctt gccgttgaaa ccgcagaaat agagctgccg
     5281 gaactggatg tcctgcgcaa taacgccacc gagttccgcg cggtgaatat cgcccgtttc
     5341 cttgataatg aagaaaactg gccgctgtta cttgatgcgc ttattcctgt cgccaatacc
     5401 tgcgaaatga tcctgatgcc cgcctgcttc ggcctggctg atgacaaact gtggcgttgg
     5461 ttgaatgaaa aactaccttg ttcactgatg cttttgccaa cgctgccgcc ttccgtgctg
     5521 ggcattcgtc tgcaaaacca gttacagcgc cagtttgtgc gtcagggtgg cgtgtggatg
     5581 ccgggcgatg aagtgaaaaa agtgacctgt aaaaatggcg tagtgaatga aatctggacc
     5641 cgcaatcacg ccgatatccc gctgcgtcca cgtttcgcgg ttctcgccag cggcagtttc
     5701 tttagtggcg gactggtagc ggaacgtaac ggcattcgag agccgattct cggccttgat
     5761 gtgctacaaa ccgccacgcg gggtgaatgg tataagggag atttttttgc gccgcaaccg
     5821 tggcagcagt tcggtgtaac cactgatcag acgctacgcc cgtcgcaggc agggcaaacc
     5881 atcgaaaacc tgtttaccat cggttcggtg ctgggcggat ttgatcccat cgcccaggga
     5941 tgcggcggcg gtgtttgtgc cgtcagtgct ttacatgccg ctcaacagat tgcccaacgc
     6001 gcaggaggcc aacaatgaat gacaccagct tcgaaaactg cattaagtgc accgtctgca
     6061 ccaccgcctg cccagtgagc cgggtgaatc ccggttatcc agggccaaaa caagccgggc
     6121 cggatggcga gcgtctgcgt ttaaaagatg gcgcactgta tgacgaggca ctgaaatatt
     6181 gcatcaactg caaacgttgc gaagtcgcct gcccgtccga tgtgaagatt ggcgatatca
     6241 tccagcgcgc acgggcgaaa tatgacacca cgcgcccgtc gctgcgcaat tttgttttaa
     6301 gccataccga cctgatgggt agcgtttcca cgccgttcgc accaatcgtc aacaccgcta
     6361 cctcgctgaa accggtgcgg cagttgcttg atgcggcgtt aaaaatcgat catcgccgca
     6421 cgctaccgaa atactccttc ggcacgtttc gtcgctggta tcgcagcgtg gcggctcagc
     6481 aagcgcagta taaagaccag gtcgctttct ttcacggctg cttcgttaac tacaaccatc
     6541 cgcagttagg taaagattta attaaagtgc tcaacgcaat gggtaccggt gtacaactgc
     6601 tcagcaaaga aaaatgctgc ggcgtaccgc tgatcgccaa cggctttacc gataaagcac
     6661 gcaaacaggc aattacgaat gtagagtcga tccgcgaagc tgtgggagta aaaggcattc
     6721 cggtgattgc cacctcctca acctgtacat ttgccctgcg cgacgaatac ccggaagtgc
     6781 tgaatgtcga caacaaaggc ttgcgcgatc atatcgaact ggcgacccgc tggctgtggc
     6841 gcaagctgga cgaaggcaaa acgttaccgc tgaaaccgct gccgctgaaa gtggtttatc
     6901 acactccgtg ccatatggaa aaaatgggct ggacgctcta caccctggag ctgttgcgta
     6961 acatcccggg gcttgagtta acggtgctgg attcccagtg ctgcggtatt gcgggtactt
     7021 acggtttcaa aaaagagaac taccccacct cacaagccat cggcgcacca ctgttccgcc
     7081 agatagaaga aagcggcgca gatctggtgg tcaccgactg cgaaacctgt aaatggcaga
     7141 ttgagatgtc cacaagtctt cgctgcgaac atccgattac gctactggcc caggcgctgg
     7201 cttaaactcc tttctgatgc ccggtaagca tgtggttacc gggcattttt gcgtacacga
     7261 ttccgtgccc aatgtatgcg ttgcaacgca gtgaaaattc ctctgaaaac gtctcgcaaa
     7321 ggctgaaact ggcagatgtc aaaggcctgg gataaccgta atgtcgcgtc atcataaata
     7381 tcaggtgacg gacaaccatg accgaatcaa caacctcctc cccgcatgat gcagtattta
     7441 aaacctttat gttcacaccc gaaaccgcac gggattttct cgaaatacat ttaccagaac
     7501 cactgcgcaa gctttgcaac ctgcaaacct tacgcctgga acccactagt tttattgaaa
     7561 aaagtttacg cgcttactac tcggatgttt tgtggtccgt ggaaaccagc gacggtgacg
     7621 gctatatcta ctgcgtgatt gaacatcaaa gctctgcaga aaagaatatg gcttttcggc
     7681 taatgcgcta tgccactgcc gccatgcagc gtcacctgga caaaggctat gacagagtcc
     7741 cgctggtggt gccgttgctg ttttatcatg gcgaaacctc gccctacccg tactcactca
     7801 actggctgga tgagtttgac gatccgcaac ttgcccggca gttgtacacc gaagcttttc
     7861 cgttggtgga tattaccatc gtacctgacg atgagatcat gcaacatcgg cgtatagctc
     7921 tgcttgaact gattcaaaag catattcgcg accgcgattt aatcggtatg gtcgacagga
     7981 tcaccacgct tttggttaga ggcttcacta atgacagcca gctacaaaca ctgtttaatt
     8041 atctgctgca atgcggcgat acctcccgtt tcacccgttt tattcaggag attgccgaac
     8101 gttcaccact acaaaaggag agattaatga ctattgctga acggctacgg caggaaggac
     8161 atcaaattgg ttggcaggaa ggtaaattag aaggtttgca ggaaggcatg catgaacaag
     8221 ctattaaaat tgccttgcgc atgctggaac agggctttga tcgtgacctg gtgctcgcgg
     8281 ccacccagct aagcgaagcc gatctggcag cgaataacca ctaattaaca cagtcccaca
     8341 gccgatcccc atgggccttt gatatcaata actacctttt atgcgtggcc catttttgcc
     8401 tgatttaaac atcgccagtc gttgatccag ggcatcgctg tagagcatcg tgtcaacgcc
     8461 aacagcgaca aagttcgctc cccacgccag gcattgctgc gccatatcag gagccacagc
     8521 cagaaaacca gccgctttac ccgcagcacg gatccgccga atgctggttt caataattcg
     8581 ctacacttcc gggtgcccgg cgttatccgg gtagcccaac gacgcagaaa gatccgcagg
     8641 tccaataaac acgccatcaa tcccttcgac gtcgaggatc tcgtccaggt tatccagtgc
     8701 cgttttactt tccacctgca ctaacagaca aagcgaatcg ttgacttgcg ccatgtaatt
     8761 ctcaatgcgt ccccagcgcg cagcccgtgc tacactggcc ccaacaccac gttcaccgta
     8821 gggaggatag cgcgtggcag acaccacctg acgtgcctga tcggcagtat cgaccatcgg
     8881 gatcagtaga gtttgcgcgc caatatccag gacttgttta atcagcggtt tactgccttc
     8941 caccggacgg atcacgggtt ggctggcata gggggctacc gcctgtagct ggtgataaag
     9001 atcctgaatg gtgtttggcg catgctcccc gtcaatcagc aaccagtcat aaccggaagt
     9061 agcggcaatt tctgccatat aggcagtcgt tgagcttaac cacagaccaa tttgcacttc
     9121 gcccttgcgt aaacgttctt taaagggatg gttaataatg cgttcatcgt gttccttaac
     9181 ctatttaatg atgtgccacg tcggtctgcg caacggtgcg atttacacgc agagtgaaaa
     9241 taatcagcga accgatgacc gccaccgctg ccagcgtcag taatcccgcc gcatcgctgg
     9301 caaataacgt ttctgctttc acgcgcagga tcggggcaat aaacccgccc actgcaccaa
     9361 gcaggtttac aaaaccaatc cccgcagcca gcgccgtacc ggaaagcagt tgtgtcggca
     9421 tcgtccagaa caccggctgc acggcaataa acccgatggc tgcaacacac agcgctacga
     9481 tcgccagtac tggagaaagc agcccggaca gaccaatacc aatgcccgcc gccagtaatg
     9541 tcagcgctgc gacattacgc cgttcgccgg ttttatcgga atagcgcgga ataagccagg
     9601 tcccaaacaa ggccgcaacc cacggaatgg cggtgaccac cgacgctgta aagcccactt
     9661 ttgttcccag caatgccgca acctgggtcg gcaggaagaa aattaatccg taaaccgcca
     9721 cctgaatggt caggtagata atcgccagtt gccagactcg cccattacgc agcgcatcgc
     9781 tcagccgaga agtcactttc tgttgttctt cacttgccag ttgattgata agcaacgttt
     9841 tttctagttt actcagaaaa cgtgcctgct ccggtgtgtc atcaagccaa aagaatgtga
     9901 ataccccagc gccgactgcc aacaatcctt caatcacaaa catccagaac cagccgggat
     9961 gaccccataa atccatgcat ctccagcagc gcgccggaaa gcggtgatcc cagtgttaac
    10021 gccagcggtg cgcccatata gaacagcccc ataatgctgg cgcgattacg ctgcggaaac
    10081 cattgcgagg tgagataaat cataccaggg aaaaatccgg cctccgcagc accaagcaga
    10141 gtgcgaacta tcagaaattt cgcttcagta tcggcccatg ccatggctgc cgaaagaaat
    10201 ccccacagca gtgttgtcgt accaatccag gttctggccc ccagtttgcg catcaaaaga
    10261 ttcgccggaa cacccagaaa cgcatatacc acaaagaaaa tgcctgctcc cagcgcataa
    10321 gcttcattac tcaacccggt atcaatctgg taggtctgtt tggcaaaacc gatattcgaa
    10381 cggtcgagaa acgccagcac atacagcgcc aacataaacg gaattaaacg cacacggttt
    10441 ttcttcacca cggcgtcaag caaagcggtg ctcataaaaa gctccttaga atatgtgggc
    10501 gttaccgttg tcacagcaac gcccggagtg attagtggct gtaggggcgt ttcaaattgc
    10561 agtcacgatt gagttcgacg ccaaaaccgg gtttatcaag cactgattta tgaatacgac
    10621 cattcaccgg aaccggctca ttgagcagaa tcgggtcaaa ctgcggacgc atcgttgaac
    10681 aatccggact agtcatcagg aattcgctga atggcgtatt agtgaaggtg atcaccgcat
    10741 ggtgggagta aacagacgaa ccgtgcggca ccaccagttg ccccctggat ttggcgattg
    10801 cggcaatttc caccagcgtg gttaagccac cgcaccagcc aacatccggc tgcataatgt
    10861 cgataccggt ttctgaaagc gtacgaaaag attgcagtgt gccgtggtgc tcaccgctgg
    10921 tgaccatcat tccgactggc gcgttgcgtt tcagttcgcg ataaccttca tactgctgtg
    10981 gcggcaggca ctcttcgatc catttcaggt tatagggcgc gcaagcgtgg gccagtttgg
    11041 tcgcatagtt cacgtcctga ctcatccagc agtcgagcat taaccagaaa tcctcaccgc
    11101 atttttcacg catatccgcg accatagcgg catctttgcg gatccccgca tcgccatcat
    11161 gtggccccca gtgcgtcggc attttgccac cgataaagcc catctctttt gccagatccg
    11221 gacgcgcacc tgtggcgtag aactgaatct catcacgaac agcgccgcct aaaagtttat
    11281 aaaccggaag cccgaccact ttgccgaaca gatcccacag agccagatcg acacaggaaa
    11341 tcgtgttcat caccaggcca ccagagccgg agtaatacag ggtggcattg agcatttgat
    11401 catggatcag tttgatatca ctgacacatt taccctcaat gaaacggtta aggtgttttt
    11461 cgacgataaa acagcccatt tcaccggctg tcgaaacagc gaatccggtc tggccgtttt
    11521 ctgcttcgac ttcaacaacc aacgtgccaa gaacgttaat gccaaatgac tggcgtaact
    11581 gctcgtaatc gcggtattta ctcatcgggg tggcaatatg atcgtctatc cagtgattcg
    11641 ccccctggtc gtgataatca ccgccgccag cgcctttttc tgctgtcgca ccgccagtaa
    11701 accaggcgcg aacctgttta atttttggta gggtcatgat gttctccatt gttatgaggc
    11761 ttgtaagtca aagggacttt tccatcccaa cagacgtgaa atatccctgg cgcaggcaat
    11821 ggccttgccc gccagataat cacggtattc ttcattgatt tgtaagcggg taccgaccac
    11881 cgagatcgca gcggtaagct cgttattggc gttaaacacc ggcgcagcga cacaacggac
    11941 atcggcgtaa tcttcgccgt tgtcatagct ccagccctga cggcgaatac gcgccagttc
    12001 ttcgtgaagt tgctgtggat gagtaatcgt tgtgggtgtc gcctgctccc agaccagccc
    12061 ttcgataata ctttgctgta cagttgcagg ttgccaggca agcaggcatt tacctattcc
    12121 ggagcgataa agcgaaaggc tttttccttc atgtgaacgc acgctgatag tggctgatga
    12181 ctccactttc aaaatgtaat aagcgctgcc gttatcaata atccccaggt gacataacag
    12241 cccggtggta tccatcagtt gcgtcaaacg cggtcgagcc agttcccgga gatccatctt
    12301 gcttaacgca tgaccggaaa gctccaccag cttggtccac aaacaaaaat tttcctggtg
    12361 atcaaggcta agaaaacgct gacgcctgag ttcattaagc agcaaatagg ccgtgctttt
    12421 agggatcccc aatgtgtcaa tgatcgtcgc agcattacag gggccaatgc gggcaatcaa
    12481 attgagaata tcaatagccc gtgtcagggc gggaactttg cttgattcca acatactgga
    12541 ctccagtctt agatactgga atcagtctcg ttgctcaggg ggagtaaaat tgtgaagcgc
    12601 atcaaatccc gctcctcgtc caacggactg gagaaattac gcacaaattt gtgaatcagc
    12661 gcacggattt tttcttattg agtacaacag gttggacaag ttttgccgga gggggaatgt
    12721 gccggtgcag taaaccggca cagagtggcg gaatcagacg gataaggtca tggactcaac
    12781 aacctcaatc cagccatgct cactggcaat gtcctggccg tttaaccaac ggcgtagcat
    12841 attcagtgcc atcattgcgc acacttcctg acggatagcc aggctatagc gtgtagtgct
    12901 gaaacgcaca cgcagagcta aagtgccatc tggcgtggct agcgcaaagt tgagatgctc
    12961 gttctcgaaa cccgaaacag ccagtgccag cccggcaaaa tggttggctc gccgttctgt
    13021 aatccagtgc gcagtttgcg ccagggtttc ttcctgtgaa ggaaccactt cacacgccag
    13081 cagtggagca cctgcgcgag aaagttgcaa agccaataaa ccaccggtga attgctcgct
    13141 caacgtcagg ctgaactggc ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc
    13201 ggtgccttcg aaaatcacgc tctgtccggc aacacgttta acgtccagcc acagtttttc
    13261 catcgcctgt tgctcgcttg ccggtccggt gagtttcagt tcgatgatcg gcattgagga
    13321 gcgatagccc attgttacgc ccggcggcag ttgtagagtg tccaggcttt gtgccagatc
    13381 gctttccgaa cgaccaaagg tagttaaacg cagacaaacc ggcggctgtg gtaaagaaaa
    13441 acgctcgcgc agacgcggca ggatttcgtg ctcgaccatc accttaaatt ctgacggtac
    13501 gccgggagta aagaacatca ggcaacgatt aagctgcacg gcaaaaccac aggcggtgcc
    13561 taccgggtta ttgataaatt cagcactggc aggcagctcc gcttgtttac ggttgctcgg
    13621 tgccattact cgtccacgtt cgtggaaata gcgttccatc tctttgagcc aggcttcatg
    13681 cagcaccagg ccttcacctt ttgctgtcgc agcggcgagt gcgcttaaat catcgctggt
    13741 cggtcccagc ccgccgttaa cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat
    13801 ggtgactaag tcatcaaggt tatcccccac cgtattgcgg cgagataatg gcaacccctg
    13861 atgaaagaaa aaatcggcca gccaggcagc gttagtgtca acgatttgcc cgtgtaacac
    13921 ttcatccccg gtggatagca tttccacttt taacatggtt atctcctgct tttgttatcg
    13981 aatcactata acgcaaaggg agataaggcg tagtaagaaa ctgaatggcg cgacagaatg
    14041 ccgcgccgga agggatcaga aactggcgtt aaccccaaca tacgggccgt cagccacggc
    14101 gttgtcgcgg ttaccgtctt tacccgacag attcaggtag cgataacccg cctcaatact
    14161 gactggacgc ataatggtgt aacgcgcgcc agcattcgct tcctcataac ttttaatacc
    14221 gctggagagt gaatccggag agtagtaata ctcgccaaac aaacggaagc tgttgccaat
    14281 tttccactgc aaaccacctc ctaccgctgc ggcatagcct tcatcgccgt aattcgggtt
    14341 ggtgtaaacg ccttttccgc caacggtcgc cattaacggc ccgagaggca gattcaaccc
    14401 cagccccacg cccgcgacgt caccgtcgtc gtcgttatgt gtccagttac cgcttaaagc
    14461 caggcccgtc gattcagtcc caaaaccgac accaatattg gtgtattctt tacccgcctg
    14521 gccgctgatg ctcattgcat tgaccgatgc agaaaccaac agcattccgg ccagacctgc
    14581 tagcgcaatt tttttcatta tcccctttcc cacaagcaat taatcaaaat tcccaaagct
    14641 gcgggattgt actgcctggc gcagaggatt caatgagcga aaaaagggat ctagtcactt
    14701 attgta
//
LOCUS       DAZFTN010000114        14614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFTN010000114 DAZFTN010000000
VERSION     DAZFTN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1742
                     /gene="espX6"
                     /locus_tag="ACHKV2_003935"
     CDS             <1..1742
                     /gene="espX6"
                     /locus_tag="ACHKV2_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFO6941730.1"
                     /translation="DKDKDISHHCNVKTEQLQQFIKIMEQKAQLPIYIDKNNLKESIF
                     SVLHNDPQQVDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQ
                     LTIRDNNNKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEI
                     IECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSER
                     LTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKM
                     ALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNF
                     KNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPQIPGT
                     PKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISNANFSNAKFYNT
                     NCTGANCSNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIVNQANN
                     STEKQSIDSTDKQANDNIDKQVNDSTDKQAKNSTEQQDSNSFNQARLKKEVNRRFSIP
                     GLTSYQPTYIVEE"
     gene            2008..2850
                     /locus_tag="ACHKV2_003936"
     CDS             2008..2850
                     /locus_tag="ACHKV2_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6941731.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            2918..4096
                     /gene="yjiJ"
                     /locus_tag="ACHKV2_003937"
     CDS             2918..4096
                     /gene="yjiJ"
                     /locus_tag="ACHKV2_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6941732.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            4307..9548
                     /gene="iatD"
                     /locus_tag="ACHKV2_003938"
                     /pseudo
     CDS             4307..9548
                     /gene="iatD"
                     /locus_tag="ACHKV2_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(9705..10300)
                     /gene="kptA"
                     /locus_tag="ACHKV2_003939"
                     /pseudo
     CDS             complement(9705..10300)
                     /gene="kptA"
                     /locus_tag="ACHKV2_003939"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            10505..11188
                     /gene="yjiH"
                     /locus_tag="ACHKV2_003940"
     CDS             10505..11188
                     /gene="yjiH"
                     /locus_tag="ACHKV2_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO6941733.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11185..11646
                     /gene="yjiG"
                     /locus_tag="ACHKV2_003941"
     CDS             11185..11646
                     /gene="yjiG"
                     /locus_tag="ACHKV2_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO6941734.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            11659..12831
                     /gene="iadA"
                     /locus_tag="ACHKV2_003942"
     CDS             11659..12831
                     /gene="iadA"
                     /locus_tag="ACHKV2_003942"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6941735.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            12953..13807
                     /gene="hypT"
                     /locus_tag="ACHKV2_003943"
                     /gene_synonym="qseD"
     CDS             12953..13807
                     /gene="hypT"
                     /locus_tag="ACHKV2_003943"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO6941736.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(13800..14192)
                     /gene="iraD"
                     /locus_tag="ACHKV2_003944"
     CDS             complement(13800..14192)
                     /gene="iraD"
                     /locus_tag="ACHKV2_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO6941737.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14189..14272)
                     /gene="idlP"
                     /locus_tag="ACHKV2_003945"
     CDS             complement(14189..14272)
                     /gene="idlP"
                     /locus_tag="ACHKV2_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO6941738.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(14388..14531)
                     /gene="ytiD"
                     /locus_tag="ACHKV2_003946"
     CDS             complement(14388..14531)
                     /gene="ytiD"
                     /locus_tag="ACHKV2_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO6941739.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         3972 a         3558 c         3420 g         3664 t
ORIGIN      
        1 aagataaaga taaagatata agtcatcact gcaatgttaa aaccgagcaa ctgcagcagt
       61 ttattaaaat aatggaacaa aaagcgcaac taccaatcta tattgacaag aacaatttga
      121 aagagagtat tttctctgtt ttgcacaatg acccacaaca agtagataaa gatcaacacc
      181 ttccctgtga aaagttttta aaacatgcct gcaaaagttc aaattcattt gaagtgaaat
      241 tagatgccac tcatcaatat caacacctga ataacttcat gatttctttt gacccagtag
      301 aaaatcaatt aaccatacgg gataacaata acaagactga aactttctcg tttacaaact
      361 tacaatggga aaatttgctg caatactaca aagaaaacca ccagcagcca aatatagcag
      421 gatcacgaaa tctcacggat aatatagata aaattaaaaa tacaatatcc acctctgaaa
      481 ttattgaatg cgcctctcct gaaataagaa gtagcgtcct gaacgatctt tatagcattg
      541 ctaatttcct cccggacaat aatctgaccc caaatgagag ctggaaaaga ttttgtgaga
      601 catgcgagcg cttttacgtt gctcagaaga gtatcactgg agataagagt gaacgtctta
      661 cgcgaaaact ctctatctct gatgcaggaa ttacaatgac cttcaagata ggtgatgttg
      721 tcatcaatac tattagcact gctattcctg aagatgcaac gggtcaacgg tgtatcgaag
      781 ggttgaattt agcagagatg gatttaaccg acatagactt gtcgaaaatg gcgctaagga
      841 atgtcaattt taatggcagc attcttagaa atgccaagtt ctccggtacg atctgtgaag
      901 gcgtggattt taccgattgt gatctgcgta atgcagaatt cgaaaatgcc tcattagaaa
      961 ataatgattt tcgtaaagtt cgccacttga cttatgtaaa tttcaaaaac gcaaatctac
     1021 gaaacagtaa cttcaacgga aaagttctca ctggcgtaac ctttactgga agtgacctta
     1081 gtaacgcgta tcttgaacac atagatttca caaccgtgat tctatatgaa acatctaaaa
     1141 tacctggaat acctggaaca cctcaaatac cgggaacacc taaagtaatt cttactggcg
     1201 caatactaaa ttattccgat ctatcgggaa aagatctttc agaatataat cttactggta
     1261 ttctctgcat gtataccaac ttttcaaacg ctaatttaac aaattgtaaa atctctaatg
     1321 caaacttttc gaatgcaaaa ttctacaata ctaattgtac tggtgcaaat tgttcgaata
     1381 tcctatttga ctacgcatgg tttgacaata caatatttat aaaaacgctt tttaaaaata
     1441 cctgttttta caatgtcaga gcgaaaaatg tctatcttga gggagcatat ctgaacaatg
     1501 ataatatcgt gaatcaagcc aataacagta ccgagaaaca atccattgac agtaccgata
     1561 aacaagccaa tgacaatatc gataaacagg tcaatgacag taccgataaa caagccaaga
     1621 acagtaccga gcaacaggac agtaacagtt ttaatcaagc ccgtttaaag aaagaagtga
     1681 ataggagatt ttccattccg ggtttaacgt cttatcagcc aacatatata gttgaagaat
     1741 agttcattaa tcacccaaac agagtgggta tggcagacga tatagattaa catttaaacg
     1801 ttaaccgcct catctatcat tcatagatga ggcgcattta tcagtcgatg taagctgaaa
     1861 atgttctgcc atccagagac aaccgccgcc ccctgcggcg tttactattt catcgcccgc
     1921 gttaagaatt cattaagctt catccgtata ctccttatct gtcgcactga atttgacgag
     1981 aaaaaagcaa taataaggag caataagttg aaaaagcgca tcacctttat cgtttttctt
     2041 tgcgccatcg tggcggcaag cctgtttttt gtgcaatcct gcgtccgtaa aagccagcat
     2101 gtcgcagggt ttcagaatta tcaggccact atcgatggta aagagattac aggagtaact
     2161 aaaaatatct cctcactgac ctggtcagcg cagagcaata ccttatttag cactatcaac
     2221 aaacctgcaa ccatcgttga aatgacgacc gagggcgact tgatccgcac cattccgctc
     2281 gattttgtta aggatctcga gacgattgaa tacattggtg ataataaatt tgtcatcagt
     2341 gatgaacgtg actacgccat ttatgtgatt tcacttaacg ctgactcgga agtgagcatc
     2401 ctcaaaaaaa ttaaaatccc attgcaggaa acaccaacta actgtggctt tgaagggctg
     2461 gcttactcct ctcaggacca taccttctgg ttttttaaag agaaaaatcc gatagaggtc
     2521 tacaaagtaa ccgggctatt gcgtagcgat gagttgcata tcagcaaaga taaaacgttg
     2581 cagcgtcagt ttactttaga cgatgtttct ggcgcggaat ttaacccaca gaaaaatacg
     2641 ttgctggtgt tgtcacatga gtcacgcgcc ctgcaggaag tgaccgtgag aggtgacgtg
     2701 atcggcgaaa tgtcgttaac caaaggtaag tatggccttt cacacaacat caagcaggcg
     2761 gaagggatag cgatggatga tagcggcaac atctatatcg ttggcgaacc gaatcttttt
     2821 tatcgcttta cgtcgacaaa atcacgataa cagtctggca ggttctcctt ttttgttatt
     2881 aacttgttaa ctcaaaataa tacaaaccag gcagaacatg aattcgacat cgcatcccgt
     2941 agaacgtttt tctttcagca ccgcgttatt cgggatgctg gttctgacct taggtatggg
     3001 tttaggccgc tttctctata cgccgatgct gccagtcatg ctggcggaag gcgagttttc
     3061 atttagcgaa ctctcatgga tcgccagtgg taactatgcc gggtatctgg cggggagcct
     3121 gctgttttca ttcggcgcgt ttcatttacc ctcacgcctg cgcccgttcc tgttagcttc
     3181 cgccctcgca accggattat taatcctcgc gatggcgtgg ctgccgccgt ttcttctggt
     3241 tttcatcatt cgctttctgg cgggagtcgc cagcgccggg atgttgattt tcggctcaac
     3301 actcatcatg caacatactc gccatccctt tgtccttgcg gcgctatttt ctggtgttgg
     3361 cgtcggcatc gctctgggta atgaatatgt gctggcaggc ctgcattttg ccctctcttc
     3421 acaaacgttg tggcaaggtg ccggagcact ttctgccatt atattgcttg ctctggcgct
     3481 gctcatcccg tcgaataaac acgttatccc gccagcgcca ttggcaaaaa tcgcgcaaca
     3541 acccatgagc tggtggttac tggcgattct gtatggtctg gcgggttttg gttatatcat
     3601 cgtcgccacc tacctgccgc tcatggcgaa agacgcgggc cagcctgtgt tgacggctca
     3661 cctctggaca ctggttggct tgtcgattgt cccaggttgc tttggctggc tgtgggcagc
     3721 caaacggtgg ggagcattac cttgcctgac cgcgaatttg ctggtgcagg cgatctgcgt
     3781 gctgttaacc ctcgccagca gctctccttt attactcatc atcagcagta ttggttttgg
     3841 cggcaccttt atgggaacga cctcgctggt gatgaccatc gcccgccagc ttagcgtgcc
     3901 gggaaatctt aaccttttgg gctttgtgac actcatttat ggtatcgggc aaattcttgg
     3961 cccggcgctg accagtatgc tcggcaacgg aacgtcggcg ctcgccagcg ccacactctg
     4021 cggcgcagcg gcgctattta tcgcagcatt aatctgcggg atgcaaatat tcaaattgca
     4081 tacgaattat tcttaagtaa tttaactaat ttaacttttc agcacatcac gtaaaataat
     4141 taaaatatcc tttttctttg gaggtactca gccaccgccg gtattattcc tggtttagca
     4201 attccacggc taattcatta tttctggtca tattatagtc aggatcgcat tatatctccg
     4261 tgctagaaaa aacaagttta attctgtttt ttcacggaag gtcactatgg cttcgacaaa
     4321 tgcgcatcaa ataaaaaata atgatcaaaa ctctttatgt ggtctgggtg acaaaatacg
     4381 ccgcctgacc gccggagttt gtttatttac acaatttttt ttccccgtca tggcgacggc
     4441 acaaaatgtg gtacatgcta aacctcagac gacagtttca tctgcccccc cctctactag
     4501 aaaataatac tgtgccttac acgcttggtg cgctggaatc agcgcaaagc gttgctaatc
     4561 gcttcggtat ttcactggag gagcttcgtc gtcttaatca gttccgtact tttgctcgcg
     4621 gctttgataa cgtgcgccag ggtgaagaac tggatgttcc ggcaacaacc tcgcagaaaa
     4681 gccatgagca acaaaatgcc gtaccgcctg cgaatggcga aaacacgctg gagaatcaaa
     4741 tagccagcac ctcgcagcga gttggcactc tgctttcaca agatatgaat agtgagcagg
     4801 ccagcggcat ggcgcgtggt tgggcgtctt cagaagcctc aggcgcgatg actgattggt
     4861 taaataactt tggtactgcg agaatctctc tgggtgtgga tgaagatttt agcctgaaaa
     4921 attcgcaatt cgacttcctg catccgtggt atgacacacc tgattatctg ctcttcagcc
     4981 agcataccct tcaccgaaca gacgatcgta cccagatcaa caccggtttg ggctggcgtc
     5041 atttcacctc cagctggatg tcaggcatca accttttttt tgaccacgac ctgagccgct
     5101 atcactcccg cgcagggctt ggcgcagaat actggcgtga ttatctgaag ttgagcagca
     5161 acgcttatat cggcctgacc ggctggcgta gcgcaccaga attggataac gacttcgaag
     5221 cccgcccggc caacggctgg gatttacgcg cggaaggctg gttacctgcc tggccacaac
     5281 tggggggaaa actggtctat gaacaatact atggcgatga agtggcgctg tttgacaaga
     5341 atgatcgtca aagtaacccc catgctatta ccgcaggcct caactatacc cccttcccgc
     5401 ttctgactct cagtgcggaa cagcgtcagg ggaagcaagg tgaaaatgac acacgttttg
     5461 ccgttgatct gacctggcaa cccagcagtt caatgcagaa acagcttaat ccggacgaag
     5521 tggccggacg gcgcagtctg gccggtagtc gttatgacct gattgatcgc aacaacaaca
     5581 tcgttctgga ataccgcaag aaagagctga ttcgcctgag tctgctggat ccggtgaaag
     5641 ggaagtctgg agaaataaaa ccgctggttt cctcgctaca gaccaaatat gcccttaaag
     5701 gctataacat cgaagccgct gcgctggaag ctgccggagg taaagtcagc acgtctggaa
     5761 aagatatcac ggtcacgctg ccaggttacc gcttcactaa caccccagaa accgataata
     5821 catggtcgat agacgttacc gccgaggatg taaaaggtaa cctgtcacgg catgaacaaa
     5881 gcatggtagt tattcaggct ccgacattaa gccagaaaga ttctctgtta tccgtcaatc
     5941 cgctaaccgt ggctgcagat aaaaaatcga cgaccacatt gaccgttact gcgcacgatt
     6001 ccgacggaac tccggtgccg gggctggcgc tgcaaacccg cagtgaaggc gttcaggata
     6061 tcaccctgtc tgactggaca gataacggtg atggtagtta cacacagata ctgaccgccg
     6121 gaacgacatc aggttcagta acactgacgc cgcaaattaa cggtgagagt gcggtaaaag
     6181 aatccatcgt cgttaatatc gtccctgttg tctcatcccg cgaccattca tcaataacaa
     6241 ttgataacgt atcgtattat gccggagacg acatcaaggt tagggtggaa ctgaaagacg
     6301 atagcaatca accggttgca tatcaaaaag aggaattggt aaaagccgtt actgtcgaaa
     6361 acagcaaacc tggcgccacg attgtctggc acgaagagca gccgggggtt tatgccgcga
     6421 attatccggc ctataagcaa gggactgcac taagggcaca acttagcctt cacaactgga
     6481 atgctccact gcaatcgcat atttataaca ttgaggcaaa ccagaataag gctcgcgttg
     6541 ccacattatc agcgacaaat aatgacgttt acgccgataa aaagacattt aataccctca
     6601 cgatcaacgt cactgatgag agtgataatc ccctgacaaa tcatcaggtc acctttaaga
     6661 atgaaaaagg aagcgcggag tttgtcgaac cgccgcagca aaatacggat gcatatggtg
     6721 ttgccacaat aaacatggta agtcaggttg cggaagaaaa tacgattagc gccacgctgc
     6781 caaatggttt ttcacaacgg ataattgcga aattcgttag cgattcgagt acgccaaaat
     6841 tcaaacaact ggttgccgat ccagatacca ttattgctgg caacagccag ggcagtactc
     6901 tgaccgccat catcacagac tttcataaca acccgttaaa agatatgaaa gtgaattttg
     6961 tggcacctgg tggctcgcaa ctggacaaca cgaccgccac aacagaccag tccggtattg
     7021 tgcgggtgca cctgaccagt tcaaaagctg gtagctattc cgtcgatgcc tcgcttgagg
     7081 tggataaaaa tattcaccag tcggtcacga tcaccgtggt cccaaacagg gaacaatcgg
     7141 taatgacctt gaatgccggg tcgggcagtg cgatcgctaa caatacaaat atcgttaccc
     7201 tgactgccag tgtgaaagat gtttatggac acccgttgcc ggatgaggat gtgaaattta
     7261 ccttgccagc ctccatgacc gggaacttca cgctaagtag tgaaaccgcc cgcaccgatg
     7321 caaacggtga tgccgtggtc acattgcgag gcacaaaagc gggtgagttt acagttacgg
     7381 cgacgctgac cagaaataat accgttgctt atcagcaagt cacttttatt ggggatacaa
     7441 acagtgcgca gctccagccg ctgactgcct cattaaattc cattgttgcg ggtaacagta
     7501 cggggagtac cctgacggca acgatcctgg acgcttacca aaatccgctt aaagaccagt
     7561 tggtcacttt ccagagtaac gatgtcactc taagcgaaac agaagtcacc accaatacgc
     7621 tgggtcaggc gacggtaaca atgaccagca atattgccgg acaacataac gtcgtggtga
     7681 gccggaaagc gcaagcttcc gataataaaa cgtttagttt atcagtgcta ccggatgaaa
     7741 gttcggcgaa ggtaataagt ataaccggag ccgaaaaaac gataacggtg ggcgaaaaca
     7801 tcacgctacg gatactcgtc caggacgcgt ttaacaatgt aatcgcgggt caacgcgtca
     7861 gattaagtgc gcagccaaca actaacatta cgataggcga tacggcttac accgataata
     7921 acggttatgc gtacgttaac cttctcagca cccaacctgg ggtttatcag gtgacggcaa
     7981 cgctggacaa taacagtagt agtaaggttg acgtgaatgt ggcaaatggc aaactcgagt
     8041 taacatcatc gaaaccagaa actacggtcc ataatagtga gggtattacg ctgaccgcaa
     8101 cggcgagaaa tgcgcggggt gaattgatgc cagggcaaat tatcaccttt agcgtaacgc
     8161 ctgaaggtgc aacgctaagc aatacagggg aagtccttac tgaccagtca ggtcaggcca
     8221 aagtgacgct gaccagtgac aaagtgaatg tctataccgt tacggccata atgggcaaag
     8281 atgttcccgt tcagagccag gtaacggttg cggttaaggc agatgctaaa acggcacatg
     8341 ttgtgagcgt cgtggcttct cctgacacca tcaccgccga cggcatcgat agcagcacca
     8401 tcacttcacg agtagaagat gattacggat tcccggttga aggtgtcgat attagtcatg
     8461 gcttagacac caaaggcagc ccggtagtta atattccaac tacgcgtacc gatcagtccg
     8521 ggcaagtcac ggcgacaata accagtacat tggcagaaac cttaacagtc aatgtgcaag
     8581 ttcctggcac agccaaccaa tccgcaacca ttacattggt tgccggcacg gccgatgaaa
     8641 gtaagtcaat tttgaaatcc gatgttgaca ctctgaaggc tgactaccag cagagcgcaa
     8701 aacttacgct aacattgcaa gacaagtacg gtaacccgat agtgacgtct gatcatctgg
     8761 aatttgtcca gtcaggcccc ttcgtgaact ttctcaagtt gagcgatatt gattacagcc
     8821 aaagaaatta tggcgagtac accgtgactg tcactggcgg aaaagaggga acagcgacac
     8881 tcattcccat gctgaacggg gttcatcagg caaacttaag catatcgctg aatctcatcc
     8941 aatcgataaa agaaatgtcc ggtcatgtca ctgcaaacaa ccataccttc tccacggcta
     9001 aattcccgag cgaaggcttt gcaggagcgt attacacact caacaatgat aactttgaag
     9061 cgggtaaaac cgttgatgat tatatgtttt caagttcaca gggttgggtg tctgtcgatg
     9121 cttcgggtaa agtttctttc gcaaatatcg gcgatcaaac gtcagtcaca ataagcgctg
     9181 ttccccgaca aggaggtaca acctaccaga ccttaattaa gctgaaaggc tggtgggtga
     9241 ataatggaaa tcataccaat atctggctag ctgccaatgc gctctgtcat gctaaaaatg
     9301 atggatataa tcttcctggc atcacacatt tgacgtctgg cgaaaacaaa cgcacgcagg
     9361 gatcactgta tggtgaatgg gggaacgttg gagcgttttc cagtaattcg caatttacac
     9421 cgggagctta ctggacaagt gaatctgatg attacagtcg gcactactat gtgcagatgc
     9481 taaccggtat gaccggaagc gacgctgatt ccagccccca actgaccgcc tgccgtaaat
     9541 cactttaatt tcagcagttg ttattaaagg ataccctttt acgagggtat cctttcagac
     9601 tgataacaaa cgctacattg ccggatgtgc tgcgcttatc aggcctatat aacccctgca
     9661 atatattgaa tttattgttt ttgtacccca gccggcataa acgactactc aagaaactca
     9721 acagcaaccg ttgatgtcag ccagaccccg ttttccgctt gccagaaagg gatgcctcgt
     9781 ttcgccattt cctgtgcttt gacggttaag acaaccggcg atccgtgtct tgctcccact
     9841 ttgcgtgccg ttgcttcatc agcagagaga tgcacgtaat gacgatcccc tgcaatcagc
     9901 ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac cgtggtagag aaactgtggc
     9961 ggtgtttttt cggcaaagga aatagcgacc tgcgaagtcg aatgcccctg aaccgcgcgg
    10021 atgcacctac catcactgga ataactaaaa cgcttttatc gctggttgcg accacggtat
    10081 ccagcaatgc acgggttaat cgcttgcccg ctttttgggc acaaagtatg agcttatcga
    10141 tattcgccca tccttcacgg tccagtacga taccaatcgc ctccggtttg tgtcggagga
    10201 caaaacttaa aaacttgctc gtatccgcca gctctttttc gttgtacttt gccatcgcgt
    10261 tttcgtccct ggattttctg tttttattta ccaatgtcat tgtcactggg tttatgaatt
    10321 ttctgcaact caatcctgcg cagaaccttt tccacacagc gtaatttttc ccctatgcaa
    10381 aaaacgcata gccagaaaca tacttgcatt ttccccatac ccacatggct gataaggttt
    10441 ttaagttcca ggaattttcc tggctcaagc aacacacaaa cacaaaaaaa caatggggat
    10501 tgttatgaca caacaagggg atgctgtagc cggggagctg gcgacagaaa aagtgggcat
    10561 taaagggtat ctggcttttt ttctcaccat aatctttttt tccggtgttt tttccggaac
    10621 agacagctgg tggcgcgttt ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc
    10681 tggtgcgaat ggagccacca cctcatttcg cggcgcgggt ggcgcggggg ctaaagatgg
    10741 ctttctcttt gcgctggagc tggcaccgtc agtcattctc tctctgggga ttatttcaat
    10801 taccgatggt cttggtggat tacgcgccgc gcagcagtta atgacacctg tgctaaaacc
    10861 gttgttgggc attccaggga tctgctcgct ggcgctgatc gctaacttgc aaaacactga
    10921 tgccgccgcc ggtatgacca aagagctggc gcaggaaggt gaaattaccg aacgcgataa
    10981 agtgattttc gccgcatatc aaaccagcgg tagcgccatc atcaccaact acttctcttc
    11041 cggcgtcgcg gtgttcgcct ttctgggcac gtccgtaatc gtacctttag ccgtcatcct
    11101 ggtgtttaaa tttgtcggag ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg
    11161 caacccaacg caaggagcgc aagcatgaca actcaggtcc gcaaaaacgt catggatatg
    11221 tttatcgatg gcgcgcgccg tggctttact atcgccacga ccaacctgct gccaaacgtg
    11281 gtgatggcgt ttgtcatcat tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg
    11341 catatctgtg aacccgtaat ggcgctgtgg ggattacccg gtgaagcggc aaccgtcctt
    11401 ttagccgcat taatgagtat gggcggcgca gtcggtgttg ccgccagtct ggcaaccgct
    11461 ggcgcattaa ccggtcacga tgtcaccgtt ttactgcccg cgatgtattt aatgggcaac
    11521 ccggtgcaaa atgtcggtcg ctgcctgggg acagccgaag tgaatgccaa atattacccg
    11581 catatcatta cggtctgcgt gattaacgca ttgctgtcga tctgggttat gcagcttatt
    11641 gtttaacaag gagttaccat gattgattat accgcagccg gttttaccct gctgcaggga
    11701 gcgcatttgt atgcgccgga agatcgggga atttgcgatg tcctcgtcgc taacggcaaa
    11761 attatcgccg ttgccagtaa tatcccttct gacattgtac cggactgcac ggttgtcgat
    11821 ctcagcgggc agatcctctg cccaggtttt attgatcaac acgtccattt gattggcggt
    11881 ggcggcgaag caggtcccac gacgcgcacg ccggaagtgg cgctaagtcg cctgacggaa
    11941 gcgggcgtca cgtcagtggt tggtctgctg ggcaccgact ctatctctcg ccacccggaa
    12001 tccctgctcg ccaagacccg tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc
    12061 ggcgcttatc atgtcccttc ccgcaccatt acgggttccg tggaaaaaga cgtggcgatt
    12121 atcgatcgtg tgattggcgt gaaatgtgcc atctctgatc accgttctgc cgcaccggac
    12181 gtttatcacc tggctaatat ggcggcagaa tcccgcgttg gcggtttgct cggcggtaaa
    12241 cctggcgtca ccgtgttcca catgggcgac agtaaaaagg cattacagcc tatttatgac
    12301 ctgctggaaa actgcgatgt gccgatcagc aagctgctgc cgacccacgt taaccgcaac
    12361 gtaccgttgt ttgagcaggc gctggagttc gcgcgcaaag gcggcaccat cgatatcacc
    12421 agcagcattg acgaaccggt cgcccctgcc gaaggtattg tccgcgccgt tcaggcgggt
    12481 attccgctgg cacgcgtcac cctcagctcc gacggcaacg gtagccagcc gttcttcgat
    12541 gatgaaggga atttaaccca tatcggcgtt gcaggttttg aaacgttgct ggaaaccgtg
    12601 caggtgctgg ttaaagacta tgatttcagt atcagcgatg ccctgcgccc gctcaccagt
    12661 agcgtagccg gtttccttaa cctgaccggg aaaggcgaaa ttctgccagg caatgacgca
    12721 gacttactgg tcatgacgcc agaattgcgc attgagcagg tatacgctcg cggcaaactg
    12781 atggtcaaag acggcaaagc ctgcgtgaaa ggaacgtttg aaacggctta aacggttgtc
    12841 agccgcccta atggacgtag aatgccccca agggcggctg acagagtaaa acgtaatgga
    12901 tgactgtggt gcggttttgc ataatattga aaccaaatgg ctttaagatt ttctgaccct
    12961 ggaaaaatgc cgcaattttt cccaggcggc agtcagtcgt aacgtctcgc aaccggcatt
    13021 cagccgccgc atccgcgcgc tggaacaggc gattggcgtt gaattgttta accgccaggt
    13081 gacgccgctg caactctcag aacaagggaa aatcttccat tcgcagatcc gccatctgtt
    13141 gcaacagtta gaaagcaacc ttgcagagct gcgtggcggc agcgattacg cgcaacgtaa
    13201 aatcaagata gccgctgcac actctctttc cctcggtctg ttaccgtcca ttatcagcca
    13261 gatgccgccg ctctttacct gggcgattga agccattgat gtcgatgaag cggtcgataa
    13321 actgcgtgaa ggacaaagtg actgtatttt ttcctttcac gacgaagatt tgctggaagc
    13381 gccgtttgac cacattcgct tatttgaatc tcaattgttc cctgtctgcg ccagtgacga
    13441 acatggagaa gcactttttg acctcgtgca gccacacttt ccgttactga attacagccg
    13501 caactcctac atggggcgat tgattaatcg caccctgacg cgccacagtg agttaagttt
    13561 cagcaccttt tttgtctctt cgatgagcga gcttttaaag caggttgccc tcgacggctg
    13621 tgggattgcc tggctgccgg agtatgccat ccaacaagaa attcgcagcg ggcagctcgt
    13681 tgtgctgaat cgggacgaac tggtgatccc gattcaggcc tacgcatacc ggatgaatac
    13741 ccgcatgaat ccagttgccg aacgcttctg gcgtgaactg cgcgagctgg agattgtgct
    13801 tagctgacat tctcaagcgt cgcactgcgc caaccgccat tatcccagac cagatcaaac
    13861 gttagcggtt catcgtcgtg gtacagcatt atctcaaggc tgatcgccag agtgtggcag
    13921 tacgcttctt tcaaattaac ctgcaacgcc gcaatccgtg gctcatgaaa taacagtgca
    13981 gaacgcagct gacggcaata ccagctctgg agatgtttgt cgctcgggtt atgcacttgc
    14041 cactcgggca tgcctgaaac aagtaatgac ggtaatgtcg aatgaggaga attaaaaaga
    14101 gtgaggagat cgctgcatac cgatttacgc agcaatgacg ttttattgcc tgaaatttca
    14161 ggtaaaacag cctgaagtga ttggcgcatc attttgcgca ctcctgacgt ttagcaacat
    14221 atgcggcttg ccctgaacac cgggcaccac tgtattgatg ctcattttcc atagccattc
    14281 ccttttgttc attacatcca tgtcagtctg agacaggcct gaatcctgtc agcaaagtat
    14341 aaaaccttac ttgcctatga atatctactg acttttcagg aaaaaagtca tgatttgata
    14401 aaaatcatca aactcaaaaa aaaaccgcta gtatattctg aatattccga atagttacgt
    14461 agatgaaaat acttgtcacc gctgctactt aattccggtg ggaaattatt tatttccgca
    14521 taatcagcca tgtattattt gaaacaatta gtttgtaaat aatataaatt gataacatat
    14581 caaatacatc aaaaacacca ttcacaggca ttat
//
LOCUS       DAZFTN010000115        14565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFTN010000115 DAZFTN010000000
VERSION     DAZFTN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(147..899)
                     /gene="tcyN"
                     /locus_tag="ACHKV2_003947"
     CDS             complement(147..899)
                     /gene="tcyN"
                     /locus_tag="ACHKV2_003947"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO6941740.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(896..1564)
                     /gene="tcyL"
                     /locus_tag="ACHKV2_003948"
     CDS             complement(896..1564)
                     /gene="tcyL"
                     /locus_tag="ACHKV2_003948"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO6941741.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1579..2565)
                     /gene="dcyD"
                     /locus_tag="ACHKV2_003949"
     CDS             complement(1579..2565)
                     /gene="dcyD"
                     /locus_tag="ACHKV2_003949"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO6941742.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2670..3470)
                     /gene="tcyJ"
                     /locus_tag="ACHKV2_003950"
     CDS             complement(2670..3470)
                     /gene="tcyJ"
                     /locus_tag="ACHKV2_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6941743.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3558..4109)
                     /gene="fliZ"
                     /locus_tag="ACHKV2_003951"
     CDS             complement(3558..4109)
                     /gene="fliZ"
                     /locus_tag="ACHKV2_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO6941744.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4155..4874)
                     /gene="fliA"
                     /locus_tag="ACHKV2_003952"
     CDS             complement(4155..4874)
                     /gene="fliA"
                     /locus_tag="ACHKV2_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO6941745.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5194..6951)
                     /locus_tag="ACHKV2_003953"
     CDS             complement(5194..6951)
                     /locus_tag="ACHKV2_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO6941746.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            7217..8614
                     /gene="fliD"
                     /locus_tag="ACHKV2_003954"
     CDS             7217..8614
                     /gene="fliD"
                     /locus_tag="ACHKV2_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6941747.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            8639..9049
                     /gene="fliS"
                     /locus_tag="ACHKV2_003955"
     CDS             8639..9049
                     /gene="fliS"
                     /locus_tag="ACHKV2_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6941748.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            9049..9414
                     /gene="fliT"
                     /locus_tag="ACHKV2_003956"
     CDS             9049..9414
                     /gene="fliT"
                     /locus_tag="ACHKV2_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO6941749.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9492..10979
                     /gene="amyA"
                     /locus_tag="ACHKV2_003957"
     CDS             9492..10979
                     /gene="amyA"
                     /locus_tag="ACHKV2_003957"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6941750.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(11013..11426)
                     /gene="yedD"
                     /locus_tag="ACHKV2_003958"
     CDS             complement(11013..11426)
                     /gene="yedD"
                     /locus_tag="ACHKV2_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO6941751.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11613..12818
                     /gene="yedE"
                     /locus_tag="ACHKV2_003959"
     CDS             11613..12818
                     /gene="yedE"
                     /locus_tag="ACHKV2_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO6941752.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12815..13048
                     /gene="yedF"
                     /locus_tag="ACHKV2_003960"
     CDS             12815..13048
                     /gene="yedF"
                     /locus_tag="ACHKV2_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO6941753.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13157..13825
                     /locus_tag="ACHKV2_003961"
     CDS             13157..13825
                     /locus_tag="ACHKV2_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO6941754.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            13936..14415
                     /locus_tag="ACHKV2_003962"
     CDS             13936..14415
                     /locus_tag="ACHKV2_003962"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6941755.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         3558 a         3727 c         3617 g         3663 t
ORIGIN      
        1 atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg ttattaatga
       61 gttatgctga ttttttaagc agttttatca ggcttgaaat ggcgttccgc cccgactgtc
      121 gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa acttctcgag gaactggcgg
      181 gtgcgaggct gctcgggatc ggcaaataac gcttttgcgg ccccctgctc gactatccgc
      241 ccctggtcca taaaaatcgc ccggtccgca acatcccggg caaagctcat ttcgtgtgtc
      301 acaatcacca tcgtgcgctt ttcctgcgcc agctgacgga tggtgttcag gacttcgccc
      361 accagctctg gatccagcgc tgacgttggc tcgtcaaaca aaatcacctc aggacgcatt
      421 gccagcgcac gcgcaatcgc aacacgctgc tgttgaccgc cagacaaacg acgtggatag
      481 ctggtttctt taccggccag cccaactttc gccaacagct tgcgagcgcg cgccgtggcc
      541 tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttctc cagcaccgta
      601 cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg caactggcga
      661 atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc gccaacggtg
      721 atcgttcccg cttccggttg ttccagcaga tttatgctac gtagcaatgt ggttttgccg
      781 gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag gtcgataccg
      841 tgcagcaccg tctgaccgtg gaattttttc actaggttct taacttcaat ggcactcatt
      901 tgggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac gtcgataaca
      961 cagtcgccat gatccagtag atgagcgaag ccgccagata catggtgaat acctccagcg
     1021 tacgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt gtcgcggcca
     1081 gcgacgtatc ttttaccaga ctgataaagc tgttcgagag cggcggcaac gccacccgcg
     1141 ccgcctgtgg caaaatcgcg cgacgcatag tctgccatgg cgtcatacca atactggcag
     1201 ctgcttccca ctgaccttta tcaatagaag aaatggcggc gcgaagcgtt tcggcagtat
     1261 aggcggcagt gttgagcgac agaccaatca ttgctgacgg aatgggatct aattcgatac
     1321 caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggc gtaccacgaa
     1381 aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac aggcgcatca
     1441 gcgcgagaat aaaccctagc agtaaaccaa aaaacatgcc gccgatactg agttgcagcg
     1501 tatacccagc ccctttcagc aggaacggca aggaatcaat aaccagttgt atactttctt
     1561 gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag gcgcgccgcc
     1621 ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga taccgtcaat
     1681 cagacccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt ccagccgcgc
     1741 cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata
     1801 gtcatcccag agtaaaattt ccactgatgc ggtcagctcc agttctttcg caatcgcctg
     1861 ttgtaggtta accactttcg gcaattgatc ggcaacggaa cgcgacacgg tcacgccaat
     1921 cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt gagttccggc
     1981 actgcccgat gcgactacca ccgatgaaat attaaccgcc ccttcacact gttgcgcgat
     2041 ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc caaccggaat
     2101 gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt
     2161 gggatcggtc agtgcgtcgc acatttcaat ctgggtattg aacagatcca gcaacaagcg
     2221 attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca gcagcgccac
     2281 gcagtgcaga ccgagtttgg cagcgactgc ggcagtctgc cgcacatggt tagactggat
     2341 cgccccggca gtaatcagcg tatcggcacc ttcacgcaga gcatctgccg cgagaaattc
     2401 tagcttacgt aatttattgc cgcccattgc catgggggtg acgtcatccc gtttgatgaa
     2461 aatttcccgt cctagataat cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc
     2521 gataaactcc agccgtggaa aacgggttaa attatgcagt ggcataacag cctccgatgt
     2581 gtgttgttgt gattttctca ttatgcacgc tgaaaacgcg taaataaaaa aggcgctagt
     2641 gaaagcgccc ttttttgtca ttatgctgat tatttggtca catcagcacc aaaccatttt
     2701 tcggaaaggg cttgcaaagt gccgtctttt tgcatttccg caattgcatc attcactgct
     2761 ttcagcaggt cttcatttcc tttacgcagc gccacgccag actcctgacg ggagaatgct
     2821 tcaccggtta ctgccagcgt atcgttggtt ttcttcacca gatccagcgc cgccagacga
     2881 tcaacgagga tcgcatcgat acgccctacg cgcagatcct gatatttggt cgggtcatca
     2941 tcataggtac gcacatcgac gccctgaaca ttctgccgca gccactcttc atagttggtg
     3001 cccagaccga cccccacttt tttgcctttc agatcatcgg ctgttttaat ggtgccttcg
     3061 ttaccttttt tcaccagcgc ctgaatacca gaaatggtgt acggggttga gaaatcgtat
     3121 tttttcttgc gctcatcaga aatggtgacc tgattaatca ccacatcaat acgtttagag
     3181 tccagcgacg ccagcatacc gtcccatttg gtcggtttta gtgacgcctc aacgccaaga
     3241 tgttttgcca gctgttgggc aaattccact tcaaaaccgg ttaatttgcc gtcatccccc
     3301 tgaaaactga acggcggata agtaccttcc agccctacca gcagcgtgcc gcgctcttta
     3361 actttattaa gcagaccttc atctgcaaaa cttttaacgc tcatgcccgc aatcagcgct
     3421 acggccatca cacccatcaa tgcctgacgt cccagatgtg ctaatttcat attcaccccg
     3481 aatgttgtta tgtctgtttg cagtgtagag ccattgtttg taaacacaaa aacaactccg
     3541 ctacatctta ttcttattta atatatatca gaagcaggca ggctggagga tttctgcaca
     3601 agtccggtac aggtttggcg ctgatagtgt tgatacttcc gtaacgcgat gcggtaattg
     3661 ttggtgctgg tggtgggcag ccacggggcg aggatttcat caaggaaacc gtcctgcagc
     3721 tgatcgaggg aaatattttg ctcgtgtaga tgattcccca aacggcgcag acgaacgaca
     3781 tattcacgca ccgtaccagg gctcatttca gtttgctcaa aaagaaattg cttaaagccg
     3841 ataatatcga aaaaatcact ctgcgttttg caatgtaaat caccgcaaaa tcggcacaat
     3901 gccgcccatg atttttgttc cgtttgccag ccattttcat caagcagcgt gtccaggcgg
     3961 gaaatctcga ttttattcac tattttgccg tcgcgaacta aggtaatgcg atcgagcaat
     4021 ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt ctttaagata gcggcttaat
     4081 ggccgtcttt tcaggtgctg caccatcatt aagaactcct ggtagtcaaa gttaaagtgc
     4141 ggcatttact gacgttataa cttacccagt ttagtgcgta accgtttaat agcctggctg
     4201 tgtaactgac tgacccgcga ttccccgacc tccagcactg caccaatctc tttgagattc
     4261 agctcttcct ggtaatagag ggttaatacc agtttttcgc gctccggcaa cgtttcgata
     4321 gcttccatca cccgctggcg cagattactg tccagtagtt gttgtagcgg gttttctcgt
     4381 tgatgatcat cagtaaccaa ttcgatgcta tcgccgtgct cttcgcgcca ctcatcgtag
     4441 gagaagagct ggctgttatt ggtgtcgagc aacatttggc gataatcggc aatatcgatc
     4501 cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc caagttcctg ctccagttgc
     4561 cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc tgcgcggcac ccagtcacgg
     4621 ctgcgaagtt catccagcat agcgccacgg atacgctgca ctgcgtaagt tgtaaatgcc
     4681 gttccttgta gggcgtcata gcgttcgacg gcattaagta acccaatgcc gcccgcctgt
     4741 agcagatcgt caagttccac gctcgcgggc agtcgaacct gcaggcgcaa tgcttcgtga
     4801 cgcaccagcg ggacataacg ctgccacagc gagtgtttat ccattacacc ttcagcggta
     4861 tagagtgaat tcacgataaa cagccctgcg ttatatgagt tatcggcatg attatccgtt
     4921 tctgcagggt ttttaatcgg acgattagtg ggtgaaatga ggggttattt gggggttaca
     4981 ggtaaattcc aggcagaaaa aaccccgccg gtggcgggga agcacgttgc tgacaaattg
     5041 cgctttatgt tgccggatgc ggcgtaaacg ccttatccgg cctacaagaa tgtgcaaatt
     5101 caataaattg caattcaact tgtaggcctg ataagcgcag cgcatcaggc aatttggcgt
     5161 tgccgtcagt ctcagttaat caggttacaa cgattaaccc tgcagcagag acagaacctg
     5221 ctgcggtacc tggttagctt ttgccagcac ggagttaccg gcctgctgaa tgatctgcgc
     5281 tttcgacatg ttggacactt cggtcgcata gtcggcgtcc tgaatacggg actgcgcttc
     5341 ggacaggttg gtagtggtgt tgttcaggtt ggtgactgcg gaatccagac ggttctggat
     5401 agcacccagg gaagaacgga atttgtcgat ggagctgatt gcgtcgtcca gggcagcaag
     5461 cgggttggtc gttgcagaac cagcactggt agtctcagta gtgattttac ccgcggagtt
     5521 cacatttaca gcagtaccaa ttgctggagc ataatcttta ttggtatcag tcgctgaagc
     5581 atacccggca acagtcacag agccgttatc cttgttaacg ctgtaagaga ctgtatagtc
     5641 ggcaacgtta gtgataccac ctttatcatc cacataagac ttcgcagcat cactggattc
     5701 acccgcggta aacccaatag ttttgctcag tacaccggaa ttaaaggtaa ttgaagatgt
     5761 cgcggcagcc gctttggttt cgctcaatac tacatcttta ctcactgtag cctgataagt
     5821 aatcccacca gggattaacg gagctactgg agtggttgtc gcaggagttg cttttgcgat
     5881 ggtatattct gtgccattga atgtcagaga ggcaccgtca ctaccttcgc tcgctgcttt
     5941 gagcagcgct ttcatatcag ctttagctgc gctaccagcg ttgttagtcg tcaagttgcc
     6001 tgcatcgtct acgtatgcct ggcttccacc gatggtgata ttacctgctg aatccgtttc
     6061 gaaagaaaca gtaccatctt ttgtggtgta agaaccatta acagatttac ccacatcagc
     6121 atgaagggtg gcagctaacg catcacgttt tgaagctgaa tcagcagcct gcgccgcggc
     6181 gtctacaccc gtaccagcag tagatttagt ggtagtaaaa tcaccagatt tagcgttgta
     6241 agtataatct acgccgccaa ctgtgacttt atcgccattc cctaatttat cgaatgcagc
     6301 atcggtagtt aacaaggtat tttcggtttt cagatcataa agacctgtcg tggtgtttaa
     6361 cttcgcgcca gcagaagtta aatcacttac cgttgcagct ttgttggtaa tagtaccttt
     6421 accatttacg ttaaagccat tcagacccag agtatcagaa tcgatttttt tcaggtcgat
     6481 cgtgatggtt tcgccgtcat tcgcaccaac ctgaattttc attgaaccgt ctttcgccag
     6541 cacgttcacg ccgttgaact gggtctggcc ggatacgcgg tcaatttcat caagacgaga
     6601 tttgatttcg tcctggatgg agtccaggtc agaatcggag ttagtccctg tagtggcctg
     6661 aaccgtcagt tcacgaatac gctgtaagtt gttgttgatt tcggacagcg cgccttcggt
     6721 ggtctgcgca acagaaatac cgtcgttggc gttacgggcc gcctgagtca ggcctttaat
     6781 gttagaagta aaacggttag caatcgcctg acctgcggcg tcatccttcg cgctgttaat
     6841 acgcaagcca gaagacagac gctcgataga actcgacagc gcagactggt tcttgttgat
     6901 attattttga gtgatcagcg agaggctgtt ggtattaatg acttgtgcca tgattcgtta
     6961 tcctatattg caagtcgttg attacgtatt gggtttccac ccgtcggctc aatcgccgtc
     7021 aaacctgtta tcgtctgtcg taaaacaacc tttagaattt ttttcacaaa cagccatttt
     7081 ttgttagtcg ccgaaatact cttttctctg ccccttattc ccgctattaa aaaaaacaat
     7141 taaacgtaaa ctttgcgcaa ttcaggccga taaccccggt attcgtttta cgtgtcgaat
     7201 gataaaagga aatcgcatgg caagtatttc atcgctggga gtcgggtcag gtctggattt
     7261 aagttcaatc cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc ccatttcaaa
     7321 tcagcaatcg tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac
     7381 gactttccag accgccaata ctgcattgtc taaagccgat cttttttccg ccaccagcag
     7441 caccaccgcg ttcagtgcca ccaccgcggg taacgccatc gccgggaaat acaccatcag
     7501 cgtcacccat ctggcgcagg cgcaaaccct gaccacgcgc accaccagag acgatacgaa
     7561 aacggcgatc gccaccagcg acagtaaact caccattcaa caaggcggcg acaaagatcc
     7621 gattaccatt gatatcagcg cggctaactc gtcgttaagc gggatccgtg atgccatcaa
     7681 caacgcaaaa gcaggtgtta gcgcgagcat tattaacgtg ggtaacggtg attatcgtct
     7741 gtcagtcaca tcaaatgaca ccggccttga caatgcgatg acgctctcgg ttagcggtga
     7801 tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg gcatggaggt
     7861 ctcggtcgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccattg agaacagcag
     7921 caacaccatc agcgacgcgc tggaaaacat caccctgaac ctgaacgatg tcaccacggg
     7981 caaccagacg ctaaccatca ctcaggacac ctccaaagcg caaacggcga ttaaagattg
     8041 ggtgaatgcc tataactcgc taatagatac cttcagcagc ctgaccaaat acaccgccgt
     8101 agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg actccacgct
     8161 gcggacgatt cagacgcagt tgaagtcgat gctgagtaat accgtcagtt cttccagcta
     8221 taaaacgttg gcgcagattg gtatcacgac cgatcccagc gatggcaaac tggaactgga
     8281 tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg cattgattgt
     8341 tggcgatggt aaaaaaaccg gcatcacgac caccatcggc agcaacctga ccagttggct
     8401 ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaagaccc tgaataaatt
     8461 aactaaagac tacaacgccg ccagcgatcg cattgacgcg caggtcgcgc gctacaaaga
     8521 acaatttacc caactggacg ttttaatgac ctcgttaaac agcacaagca gctacttaac
     8581 gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga gacaggtcat
     8641 gtacgcggca aaaggcaccc aagcctatgc acaaattggc gtcgaaagcg ccgtaatgag
     8701 cgccagccag cagcagctgg tcaccatgct atttgatgga gtgctgagcg cactggttag
     8761 agcgcgcctg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc
     8821 gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca aagacgaact
     8881 aacccaaaac ttgattgctc tttatagcta tatggtcagg cgtttgctgc aagccaattt
     8941 acgcaacgat gtctccgcag tcgaagaagt ggaaacatta atgcgcaata ttgccgatgc
     9001 ctggaaagag tcgttactct ccccttcttt gattcaggac ccagtctgat gaaccatgca
     9061 ccgcatttat atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg
     9121 gcaacggaag aacaatggga cgaactcatc gccagcgaaa tggcgtatgt taatgcggtg
     9181 caggagattg cacatttgac tgaagaggtt gacccgtcca ccacgatgca ggagcagctc
     9241 cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt
     9301 cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttgagt
     9361 gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt ttgaatctga
     9421 atgagtcgat ggctcgcgaa taatccgatt acggctacgc ttctaatgtt ccccttgaat
     9481 ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag
     9541 gcggtaagct ctggcctgaa ttggccgagc gcgccgacgg ttttaatgat attggtatca
     9601 atatggtctg gttgccgccc gcctataaag gcgcatcggg cgggtattcg gtcggctacg
     9661 actcctatga tttatttgat ttaggtgagt ttgatcagaa aggcagcatc cctactaaat
     9721 atggcgataa agtacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg
     9781 tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg
     9841 tgcagcgcgt taatgctgat gaccgtacgc aaattgatga agaaatcatt gaatgcgaag
     9901 gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcacagttt atctgggatt
     9961 tcaaatgttt tagcggtatc gaccatatcg aaaaccccaa tgaagatggc atttttaaaa
    10021 ttgttaacga ctacaccggc gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt
    10081 tcgattatct gatgggcgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta
    10141 aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggttttcgt cttgatgcgg
    10201 tcaaacatat tccagcctgg ttttataaag agtggatcga acacgtacag gaagttgcgc
    10261 caaagccgct gtttattgtg gcggagtact ggtcgcatga agttgataag ctgcaaacgt
    10321 atattgatca ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc
    10381 atgaagcatc gcgcatggga cgcaactacg acatgacgca gattttcact gggacgctgg
    10441 tggaagccga tccttttcac gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc
    10501 aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgct ctaattttgt
    10561 tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgcg cattacgaag
    10621 atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
    10681 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacgttattt ttcgaccatc
    10741 cgaactgcat tgcctttagc cgcagtggca ccgacgaata tcccggctgc gtggtggtca
    10801 tgtcgaacgg agatgatggc gaaaaaacca ttcatttagg agagaattac ggcaataaaa
    10861 cctggcgtga ttttcttggg aaccggcaag agagtgtagt aaccgacgaa aacggcgaag
    10921 cgaccttctt ttgcaacggc ggcagcgtta gcgtgtgggt tatcgaagag gtgctttaaa
    10981 ttcatccccg gcggcaagcc ggggagattt cattacggca atggcgtcgg taacggcgct
    11041 ttatccagcg cagcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
    11101 tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt tacggtaacg
    11161 ttagtgagat catcagacat caatgtcaac ttgcccggca cagcaatcac ccgctgccac
    11221 tgacggcaat ctagggtatc gccctcttta gtgacaatca ggctgcctat cgcttccggg
    11281 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg
    11341 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
    11401 gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
    11461 gcattaaagc cctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
    11521 tatttttgtg aattattacc ccgaaaataa atttgcatgg tgtatgattc gcgactcttg
    11581 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
    11641 ggttgattaa attctgggcg cccatccccg cggtcatcgc ggcgggtatt ctctccactt
    11701 actattttgg catcactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
    11761 gtcagctcct gcagctgttc ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
    11821 atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggt atgtttggcg
    11881 gctgctttgc cgcagcgctg tgggccaaca atgtcaaact acgcatgccg cgtagccgta
    11941 tccgcattat gcaggccatt attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
    12001 tgggctgtaa cctggcggca ttctttaccg gtattcctca gttctcgctg catgcctggt
    12061 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
    12121 tcttccgtat tcccgtgaaa atgcagaaag ttgctgccgc ctcaccgctg acgcaaaaac
    12181 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
    12241 gggcgctgct cacagcgatg aaccaaccca aactggggct ggcaatgctg tttggcgtcg
    12301 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
    12361 ggatcaccgg acgtacccat atggcgaaag caatcattat tggtatggcg gtgagtgcca
    12421 tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa
    12481 acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggctggc ggctgcgaaa
    12541 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
    12601 atgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
    12661 ccgactggga caaaatcaac ttgctgaaaa cctttggtcc gatggggggc ctgctggtga
    12721 catatttgct gttgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
    12781 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
    12841 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
    12901 gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
    12961 tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc
    13021 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtgaccc acagcgggaa
    13081 catcgttacc tttgtatcac atagatttta cccgccccct ctcctattct tttcagtatg
    13141 atctcaggag gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct
    13201 tgcgcttctc gcggaagata ttgaacgcga tattccctat gatcccgaac ccattggcag
    13261 atacaacgtc gcgccgggaa ccaaagtcct actgctcagt gaacgtgatg aacaccttca
    13321 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
    13381 cgcccgcgta gaaactgcgg caaccagtcg tatgtttaaa ccgctctggc aacatggtcg
    13441 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaagcagcc
    13501 ttattttatc tatcgtgctg atggacaacc tgtttttatg gcagcgatag gcagcacacc
    13561 gttcgagcgc ggtgacgaag ccgaaggatt tttgattgtc actgcagcag cagaccaggg
    13621 tctggtagat attcatgacc gccgcccgct ggttctgtcg ccagaagctg cgcgggaatg
    13681 gatgcggcaa gagattagcg gtaaagaagc ctcagaaatc gcggccagtg gctgtgttcc
    13741 agcaaaccag ttctcctggc acccggtatc gcgcgcggtg ggtaatgtta aaaaccaggg
    13801 tgcggagtta attcaacctg tttgataagc ctggagatta ttgatcaaca atactgcgtc
    13861 ataagtaatc tctattagac aaagatttca ttacctgttg gcatattgca aaaataacac
    13921 caatacggaa tcgtcatgtt cacgattaaa acagatgatc tcacccatcc agcagtgcaa
    13981 gcattagtgg cttaccatat ttccggcatg ctgcagcagt ctccccctga aagcagtcat
    14041 gctttagacg tgcaaaaatt acgtaacccg acagtgacat tctggtcagt atgggaaggc
    14101 gaacaactcg caggaattgg tgcgctgaag ttgctggatg ataaacatgg cgaactgaaa
    14161 tcaatgcgga ccgcgccaaa ttatttacgt cggggtgtcg ccagtctgat tttacgccac
    14221 attttgcagg tcgcccatga cagatgcctt catcgcctga gtttagaaac gggtacacag
    14281 gctggattta cggcctgcca tcaactttat ttgaagcatg gtttcgttga ttgcgaaccg
    14341 tttgccgatt atcaacttga tccacacagt cgatttttgt cattgacgct atgcgaagat
    14401 aatgagttgc tttgagccag acgcagcaca ttcttgcatt cgacgtgctg cgtctttatt
    14461 tatcaccaac aggaaacgcc ttgtccatag acgccccttc cacatgcgtc acaagaaacc
    14521 tctattccag tgacacaatt acgcctaatt aattacatat aatat
//
LOCUS       DAZFTN010000116        14488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFTN010000116 DAZFTN010000000
VERSION     DAZFTN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHKV2_003963"
     CDS             complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHKV2_003963"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO6941756.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHKV2_003964"
     CDS             complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHKV2_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO6941757.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3791..5146
                     /gene="melA"
                     /locus_tag="ACHKV2_003965"
     CDS             3791..5146
                     /gene="melA"
                     /locus_tag="ACHKV2_003965"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO6941758.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5249..6670
                     /gene="melB"
                     /locus_tag="ACHKV2_003966"
     CDS             5249..6670
                     /gene="melB"
                     /locus_tag="ACHKV2_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO6941759.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLLAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVVDTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHKV2_003967"
     CDS             complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHKV2_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO6941760.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHKV2_003968"
     CDS             complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHKV2_003968"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6941761.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEKAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHKV2_003969"
     CDS             complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHKV2_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO6941762.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLD
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHKV2_003970"
     CDS             complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHKV2_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO6941763.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHKV2_003971"
     CDS             complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHKV2_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6941764.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHKV2_003972"
     CDS             13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHKV2_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO6941765.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHKV2_003973"
     CDS             13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHKV2_003973"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6941766.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         3480 a         3470 c         3781 g         3757 t
ORIGIN      
        1 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
       61 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      121 agcggaatag tggttacgct ttcacacaca taacgtggta aataccgtca ataatttcag
      181 tcccttcagt ttcgtgttca aatccaggga aatgatggtc ccaggattgc agcgagcgta
      241 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      301 ttcctggcgg atacgggata actgagtttg ccgcgatacg tcctggcaga ttttcaatgg
      361 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
      421 ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      481 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      541 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      601 aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca
      661 ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg
      721 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      781 gagcaaggat gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga
      841 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      901 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
      961 tgttccacgg tttgaagaac cagctaccgt cagcggcgaa ctctttatac agccgcgcca
     1021 tcgcctgacg gaaatcaacc gcttcgtcga tcacttcctg cgtcagtgac aggccgctgt
     1081 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1141 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1201 cacgtacatg aatataagag gcctgtgaaa gcgcattcag cagtttgtgg gtggagtggg
     1261 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1321 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1381 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1441 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttagtc agcgggcttt
     1501 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1561 tgccgtagcg gttgcggctt ggcaccatgt agaccggttt cgcgcctgtc agcatcaaac
     1621 cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc
     1681 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1741 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1801 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccgt
     1861 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1921 ctggcgctgc ccaggaatat tcatggatgt cgctatattt catcagcgcg ctgaacagtg
     1981 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
     2041 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca
     2101 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2161 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2221 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2281 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2341 cagcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2401 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2461 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttgtttttat aaacaatatg
     2521 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2581 gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc
     2641 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2701 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2761 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc
     2821 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2881 tggtcagcgc ctggtcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     2941 gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt
     3001 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3061 gaatttgctc attcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3121 gttgctgtgt agcaagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3181 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac
     3241 aggttccggt atctgttagt tggtgcggta tacaggccca gaacagcgtg atatgtccct
     3301 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3361 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3421 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3481 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3541 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3601 gggcgagggg agtgtccgag catcatgagg ccgaaaactc tgcttttcag gtaaattatt
     3661 cccataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3721 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3781 ggagatctgc atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3841 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3901 cctgatggac attgacccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat
     3961 ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca
     4021 ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgcacctt gcacggtgac
     4081 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4141 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4201 gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac
     4261 ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca
     4321 gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccggctacgc tgcgttaccg
     4381 ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg
     4441 cagttacgtg aatctctacc cggaactgct ggcggcttat gacgcagggc aggcaccgaa
     4501 gccgaatatt cacggcaata ctcgctgcca gaatattgtg cgctatgaaa tgttcaaaaa
     4561 gctgggctat ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat
     4621 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4681 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4741 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4801 gggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4861 acaaggatgt tgcgtggaag tggcctgtct ggttgatgct aatggcattc agccgaccaa
     4921 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     4981 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ctgcgatgat
     5041 ggacccgcat actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct
     5101 gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5161 ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg
     5221 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5281 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5341 ttactacacc gatgtcgtcg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5401 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5461 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5521 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgcct ttgtttgcgt
     5581 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5641 tccaaccatc acgctcgata aacgtgagcg tgaacaactg gttccttatc cgcgtttttt
     5701 tgccagtctg gcgggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5761 cggtggcgat cggggatttg gctttcagat gttcacactg gtactgctcg ccttttttat
     5821 tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5881 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgcttattt ataaaaacga
     5941 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac
     6001 cggctttgct atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta
     6061 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6121 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6181 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6241 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggtaga
     6301 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagcatcg cttactccgt
     6361 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6421 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6481 gtttattatg attgctctac cgactctgtt tttcatggta acgctgattc tctacttccg
     6541 tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata
     6601 tcgcaaaata ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6661 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
     6721 acgtcttaat ggaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6781 gcatccggca tcaacaaagt gcacgttgtt atccggttat caagccaaag cgccgtagct
     6841 gacggcaatg aaagcgagcg aggaatatca ccgtggttaa tgcgccaagc agcgcgcaga
     6901 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     6961 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7021 ccacgcaaca gaccagaaac gccaccattt tacgtccgcg aacgtacatc ccgcgcacaa
     7081 ggatctctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7141 acggattacg gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt
     7201 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7261 gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg
     7321 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
     7381 ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     7441 tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7501 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7561 ttacttagtg cagttcgcgc actgtttgtt gatgatttgc tggaagaagt cgttaccttt
     7621 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7681 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     7741 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7801 ctgctgactg cggttacctt tcgctagcat gatcatgctg ccgccgtggg attgcagcag
     7861 atccacatag gagtccatac gacctgcggt ggttgggcca agtgagcctg atggataacc
     7921 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     7981 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     8041 aatggtgccg gtgagcgaca aacgagtgga aaccggatat tgcgaaagct gggcgaggat
     8101 ctctttcatc gggcggttaa ggtcgacctt caccgcttcg ccttcaccgg cctggcgcag
     8161 ttcttgtgga atgtactgac ctggatggtg ttccagtttt tcgatccaga taccttcgcg
     8221 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc ctaccgggca
     8281 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     8341 aaactgcgcg ccaagaccga gtttctgggc cttttccagc agttcctgtt ccagctggac
     8401 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     8461 gcttgctaac ttaacggttt tcaggttggt ttccacagac gtaccgccaa tcacaaacgc
     8521 gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt
     8581 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatagg ttttgttggc
     8641 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     8701 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     8761 gttttgcgag tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     8821 ttcttcatca ccgccaccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt
     8881 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa
     8941 ttgcagtgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     9001 ctgtttctgg tgtgccggac ggagcatgaa agaagcgtcg tggaaggctt gctgcgccag
     9061 cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     9121 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     9181 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     9241 gaaaatggcg tgccgcgtag taacgggcgg cacgcaaagt gcatttataa gaacccgtac
     9301 atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga
     9361 ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     9421 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     9481 gaagccacga tgtatgccgg atcaacgccg atcgctagcg cgaccggaac aatcgccgcc
     9541 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcataggcc
     9601 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     9661 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     9721 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     9781 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     9841 aaggatggac gcaggtccga atcagcacca agaagtgcga ctacagcgat tgccccgagg
     9901 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag cgtcgcggta
     9961 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
    10021 ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac
    10081 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
    10141 acattatcca gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
    10201 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
    10261 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
    10321 atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
    10381 agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
    10441 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ctggtggttt acctggctga
    10501 aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
    10561 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
    10621 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
    10681 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
    10741 ctttagcaaa tacctcacag tgaatattgg ctgattaaag cagcggcaaa tttatgcact
    10801 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttaaa gtttgtactg
    10861 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
    10921 tctcagggca gatatcatta ggtttataga ttaatctgat ctacccattt gtgggtaaaa
    10981 atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa
    11041 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
    11101 atctggcaca agttttactg atgaaggatg taacttgtac caggggtatt ttgcattacg
    11161 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt
    11221 gagtagtgtt ctgcctgaat acggtaacgg taaactggac gccccgtgac gccataatgg
    11281 atactggtga acaagatgtg gcagttgacc agccagataa ggtatttacg gcaggaaaca
    11341 cgcgaaatgt taacctcgtt ggctagctcg tcggttgaaa actcatagtc ctgatgcgcg
    11401 tcaatccact ggcacagtgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga
    11461 ggatcctgct cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
    11521 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
    11581 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
    11641 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
    11701 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
    11761 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
    11821 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
    11881 attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg
    11941 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
    12001 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
    12061 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat
    12121 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
    12181 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
    12241 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca
    12301 gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg
    12361 gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga
    12421 gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
    12481 atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt
    12541 cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga
    12601 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg
    12661 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
    12721 gtgaccatga gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
    12781 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
    12841 cgccttcttt gatagactgc aacatggcct ggcgttgctc aaacagcgtg gagatttcgt
    12901 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
    12961 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgatct
    13021 gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt
    13081 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
    13141 cattttcttc gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac
    13201 gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca
    13261 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat gccgctctcc tgcggttttt
    13321 tctgcaagcc ctgacggatt tccggcgagt cggcgagagt acgcgccact gccagtgcct
    13381 tgttggctag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca
    13441 ccaatagcac cgagaacagt accgcgctga ccattaagat cactgtggta ctcaatttca
    13501 tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
    13561 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
    13621 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
    13681 ctatgcttat agtgaaaacc atactgatga gagagggaag gtcatggatc aggcgctact
    13741 ggacgggggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
    13801 atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa
    13861 gccgtggatc atgccggatg aagtcgacgt tgccactgtg gttaaagtga ttgatacgtg
    13921 cccgagtggt gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
    13981 ggccacaata aattttacat taatgacgaa caaggcaagc aaatcgctga aattgtcttt
    14041 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
    14101 gggcaaggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
    14161 cgaaaaatta tcccattatg tccatttgcg aaacacgaat ttgataaaac gcgggagtat
    14221 gatgatattc gcagttgatg gaagagtaca gattcacgat atttttcatg ctctccgcgg
    14281 tgtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg
    14341 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
    14401 atcatcaatc ggggttaata aattttgcgt ccccagagtg ttcaatattt ataggagtca
    14461 tattatggaa ggtaaaaaca agttcaat
//
LOCUS       DAZFTN010000117        14392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFTN010000117 DAZFTN010000000
VERSION     DAZFTN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..462
                     /locus_tag="ACHKV2_003974"
     CDS             106..462
                     /locus_tag="ACHKV2_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6941767.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(513..1409)
                     /gene="yhaJ"
                     /locus_tag="ACHKV2_003975"
     CDS             complement(513..1409)
                     /gene="yhaJ"
                     /locus_tag="ACHKV2_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6941768.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1514..2215
                     /locus_tag="ACHKV2_003976"
     CDS             1514..2215
                     /locus_tag="ACHKV2_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO6941769.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2238..2402
                     /gene="yhaL"
                     /locus_tag="ACHKV2_003977"
     CDS             2238..2402
                     /gene="yhaL"
                     /locus_tag="ACHKV2_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO6941770.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2536..3846)
                     /gene="cyuA"
                     /locus_tag="ACHKV2_003978"
     CDS             complement(2536..3846)
                     /gene="cyuA"
                     /locus_tag="ACHKV2_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO6941771.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3874..5205)
                     /locus_tag="ACHKV2_003979"
     CDS             complement(3874..5205)
                     /locus_tag="ACHKV2_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6941772.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5480..6844)
                     /gene="tdcG"
                     /locus_tag="ACHKV2_003980"
     CDS             complement(5480..6844)
                     /gene="tdcG"
                     /locus_tag="ACHKV2_003980"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6941773.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCEPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6916..7305)
                     /locus_tag="ACHKV2_003981"
     CDS             complement(6916..7305)
                     /locus_tag="ACHKV2_003981"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO6941774.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7319..9613)
                     /gene="tdcE"
                     /locus_tag="ACHKV2_003982"
     CDS             complement(7319..9613)
                     /gene="tdcE"
                     /locus_tag="ACHKV2_003982"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO6941775.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9647..10855)
                     /gene="tdcD"
                     /locus_tag="ACHKV2_003983"
     CDS             complement(9647..10855)
                     /gene="tdcD"
                     /locus_tag="ACHKV2_003983"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO6941776.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10881..12212)
                     /gene="tdcC"
                     /locus_tag="ACHKV2_003984"
     CDS             complement(10881..12212)
                     /gene="tdcC"
                     /locus_tag="ACHKV2_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO6941777.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12234..13223)
                     /gene="tdcB"
                     /locus_tag="ACHKV2_003985"
     CDS             complement(12234..13223)
                     /gene="tdcB"
                     /locus_tag="ACHKV2_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO6941778.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13322..14260)
                     /gene="tdcA"
                     /locus_tag="ACHKV2_003986"
     CDS             complement(13322..14260)
                     /gene="tdcA"
                     /locus_tag="ACHKV2_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO6941779.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3723 a         3857 c         3359 g         3453 t
ORIGIN      
        1 cttaaatatc aaaaccttta taacatccga aaaagaaaga tacgaatagc atttacacaa
       61 tacccggaat agactttcgt cacttttaat taaagggatg tttttatgca gtggtattta
      121 tccgtattaa aaaattatgt tggtttctct ggtcgcgcac gtcgtaaaga gtactggatg
      181 tttactctga ttaacgccat cgtcggcgct attatcaatg tcattcaatt gattttaggt
      241 ctggagcttc catatctgtc tatgctttat ttgctggcaa ccttccttcc cgtccttgcg
      301 cttgctatcc gtcgcttaca cgataccgac cgttcaggtg cctgggcctt gctctttttt
      361 gttccgttta ttggctggct cgtgcttctg gtttttttct gcacagaagg tacttctggc
      421 agcaatcgtt acggaaacga tccgaagttt ggttcaaatt aatcttagaa ttggggcgat
      481 atttcgcccc tttttattaa caataataaa tattattttc cgttaaaaag tttgggaatt
      541 tcccgcagac accaggattt cgcttccccc atactgtcac gacgccaggc cataataata
      601 tcgatctcgc tggtcgattc cgggctgacg acacgcaacc gcccttccgc aatatctttt
      661 tcgaccatcg gatacggcat cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta
      721 tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca gcagctgtac agtcaacacc
      781 gggcgctcac gagcggtatc cgccaccgca attccacgat atttcacacg cgtcacttca
      841 gataacggtt ccggctcctg atgaatcggg tgatcaggcg cggcaacgta gacgttcatt
      901 aacgtataga gcttgcgcga gttgatctcc gacgaggaac gaaaatgcat atccggcgcg
      961 ataacaatat ccgcccgccc ctgctccagc cgttcccacg cccccgccag cacttctgtg
     1021 atgattgcca gttgggtatt ggcttttgcc gccagtttgt cgattaacgg gaaaaaggca
     1081 ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg tttcccaacc gcgcgcgaga
     1141 gcttccgcat cagtagtcag tttatctgcg gcttccagca aaacgcgccc tcgctccaac
     1201 aacatcctcc cgacattggt gaatttggtg cgatggcccg agcggtcaaa cagcaccaca
     1261 tccagctctt cttccagttt ttgcatggtg tagctaagtg cggaaggcac gcgtcccagc
     1321 tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg catccataac ccgtagtgct
     1381 tccagcgtta atgccctttc tttggccatt tcgttctcat tcaggaaatt tgaacatacc
     1441 gggcagaata tctggctaac aatgtagcgt ccagcccctt accataaaag gaagtaaaga
     1501 gaggtcaaga attatgatta ctacccgaac tgccaggcag tgtggacaag cagactacgg
     1561 atggttgcag gcccggtata ctttttcctt tggacactac ttcgacccga aattgttagg
     1621 ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc ccaggtgccg cctttcagcc
     1681 gcgaacctat cccaaagtcg atattttaaa tgtgattctg gatggagaag cagagtatcg
     1741 cgacagcgaa ggcaatcatg ttcaggccag cgccggtgag gcgttgctgc tctctaccca
     1801 gccgggtgtc agctacagcg aacacaatct cagcaaagac aaaccgttaa cacgaatgca
     1861 gctttggctg gacgcctgcc cgcagcgaga gaatccgctg attcaaaaac tggcgcttaa
     1921 tatgggcaag cagcatttaa tcgcctcgcc agagggtgcg atgggaagcc tgcaattacg
     1981 ccagcaagtg tggctgcacc atatcgtgct cgacaaaggc gaaagtgcga atttccagtt
     2041 gcatgggcca cgcacgtatt tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca
     2101 tgaagagaaa gcggcgctga cctgcggtga tggggcgttt attcgtgacg aggctaacat
     2161 tacgctggtt gccgattccc cactgcgcgc tttgctgata gatttgcctg tctagttgtt
     2221 tttacaggga gatgatgatg agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg
     2281 tggtggcgaa agaacctgct cgcaccgcca aaaattttgg ctatgaagag atgttgagcg
     2341 agctggaagc catcgtcgcg gatgctgaaa cgcgtttagc cgaggatgaa gctaccgcgt
     2401 aaagacaggt tgccggatgc ggcgaaaacg ccttatccgg cctacaaaat ccaacaacat
     2461 taacgaatta aacaacccgt aggccggata agatgcgcca gcatcgcatc cggcacgata
     2521 cccaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt
     2581 ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg
     2641 cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt
     2701 ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg caccatcgca
     2761 aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatgg tttcataacg
     2821 cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca
     2881 cagcgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat gagaaagcat
     2941 cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacggg
     3001 cattgttgcg gtgatcccct ggttacccga gccggagtta ctcattgccg gaagcgtggc
     3061 gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc
     3121 tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat gcagccccca
     3181 cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat cgagaataaa
     3241 gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct
     3301 ggaaagcacc gatagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa
     3361 caccacacca tcgtgcgtct caatatgcac aatgttggta tgcccgccga cgatagtgac
     3421 acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg
     3481 ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc
     3541 ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc
     3601 cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc
     3661 gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc
     3721 tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg
     3781 ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc
     3841 aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac
     3901 acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa
     3961 tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccaagc cgaaaatcgg
     4021 gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc tccaggccag
     4081 caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg
     4141 catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa aacctaaata
     4201 gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac
     4261 ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg
     4321 ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa
     4381 aaagacgatg ataaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
     4441 cgctacttca atcgattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
     4501 gataaacaga atcgacgtca gggtaaacgg cagcgtaata atagcgtttt tcaccagcag
     4561 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
     4621 cagcgccgcg accaccagca gcttggtcag caccatgcca gtcgaaattt tgaataacaa
     4681 tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
     4741 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag cactatcgtt
     4801 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agagtgcacc
     4861 taacaggatg ccccagtttt tacctaaata accgctaatg acgctcgggt aatctttaca
     4921 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
     4981 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
     5041 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
     5101 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
     5161 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat
     5221 attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
     5281 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
     5341 atcaagactt tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
     5401 atattcactc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg
     5461 atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtccg ccgcgtgatg
     5521 tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg atcactttat
     5581 cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt
     5641 tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg gcaaccggct
     5701 cgcaggtcag tccaaggtta tgctccatcg cgatttccgc cgcattgcat acctgcgccg
     5761 gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc acgccaatct
     5821 ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac agcgcgccaa
     5881 tagccccggc tgcaagaaaa tagcgggcaa ttgaccgctc gtttaccgga cgacggaact
     5941 tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc ggtgccgtta
     6001 ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg ttgatccagt
     6061 cgatgacatt catcggatcg ttagagatgt tatcgctgga aaccagctga cgacgcagcg
     6121 ctacggcacg gcgcggcaca ttgagcggac caggcagcac gccttcagtg ttcatcccgc
     6181 gttcaatacc gtcatgcatc acttgccaga tacgggcaaa accggcgtca atttccgctt
     6241 tactgcgcag cgctagctcg ttgtgcatca tcaggccaga aatagacagg ccgttgtaat
     6301 cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacgggt gtttcgacat
     6361 cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca gagtaatagg
     6421 ttttacttaa tagctcttcc tgtgccttcc aggcagtgat ccgcattccg ttctcatggc
     6481 gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca acaatatgcg
     6541 caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat gcagggatct
     6601 catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgatgatg gcgacatccg
     6661 tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca atatggctcg
     6721 tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg gcattcattg
     6781 gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg aatgcactaa
     6841 tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc ttacgaccgt
     6901 cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca gcttcacatc
     6961 tttcggcaaa cgcgcgacct gtacacagct ccgggtcggg taggtcgcct gatgctcatc
     7021 gaagaattgc ttatagactt cgttgatggt ggcaaaatca ttcagatcgg tgatgaacac
     7081 ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg ctttgacgtt
     7141 ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg tctgtgggca
     7201 aaccggaatt tgaccggagg tgaagaccat gctgcctaaa tcaacgcctt gaacataagg
     7261 gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc ctccggcgtc
     7321 agagcgcctg ggtaaaggta cgtgaaataa catcctgctg ctgttcacgg gtcagtgcgt
     7381 tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt tccgggtgct
     7441 cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt tgaccgcctt
     7501 cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg gttttacgta
     7561 ctggatcttc tttgcccagc gccgcaggga cgattgagaa ggtgtacgag atcccatctt
     7621 tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca cctttgcggt
     7681 cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg
     7741 gcgtgttacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga atcgactggg
     7801 tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaaa cgttcaacca
     7861 ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt
     7921 cgatttcaaa gtccaccgcc aggccgtttt tgtcacggat tggtttcacg cgggcatatt
     7981 tgatcgcaga cagggagtcc gttgccaccg acaggcccgc gatgccgcac gccatggtgc
     8041 gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca
     8101 tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc atgaagtgat
     8161 cgaggctgtc catcactttg tcgtaatcca gcacatcgtc catcaacggt gccgttttcg
     8221 gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtac agcagtgttt
     8281 tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc attgggctga
     8341 cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga tcgtcgtttt
     8401 catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg aaggcaatcg
     8461 gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgcaggcccc atagtgtgca
     8521 gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc agccccatcc
     8581 cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca aattccggcg
     8641 tacgcaagaa gcgcaccata cggatcttca tgatgaagtg atcgatcagt tcctgtgcct
     8701 gctgctcatt gagtacgcca gctttaaagt cgcgctcaat gtagatatcc aggaacgatg
     8761 ccgtgcggcc cagcgacatc gcgccgccgt tctgtgattt cactgccgcc agataagcga
     8821 agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata tcaaagccat
     8881 atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc agctcctcac
     8941 gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg agatcggcaa
     9001 attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg cggcgatagt
     9061 caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg ccagatttac
     9121 ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg gttttacgca
     9181 gatcggtaaa cagatattca aattcactgt ccatttcacg accataggcg tggaatgaac
     9241 ttttaatcat attaatgcca ccgaacgggt gtaacgcacg tttcaacggc gcatccgttt
     9301 gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga gcggtaattg
     9361 tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttca atacgaatgc
     9421 cttccatcac cttttcccac aattccgtgg tggctggcgt cgcttcagcg aggaaagatt
     9481 catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta atttcgtttt
     9541 tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa
     9601 tatctacctt catgaaaaat aatctctcta caatacttca actaaattat gcaaattctg
     9661 cgggtgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt tcttcgttgg
     9721 tcggaattac ggcgcaaatg acacgcgcat tttcactgga aacaattcgc tcgccaaaag
     9781 agttagatcg attattcatt tctgtatcaa tctctacgcc taatacagcc aaatgttcca
     9841 tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat ataattccat
     9901 ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg
     9961 ttttaattgc cagctgcgcg cgttcgtgac cttcatgcca ggctttttcc agaacacgta
    10021 aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact acgcgttcca
    10081 ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca aagtcgacat
    10141 cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt gaggtgtcaa
    10201 cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc gccacaacca
    10261 ggccggagtc atcttccgcc agattcagca aagaatgtgc gcgcagggaa acatagcggt
    10321 gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat ttccacggca
    10381 ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca aataccgcca
    10441 cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa ttggcgtaat
    10501 tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca tcggtaataa
    10561 tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt aaggccacgc
    10621 tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca ccttcgtagc
    10681 tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca ttttccgagt
    10741 taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg agtacggaaa
    10801 acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca ttcatctctt
    10861 ttctcatcct gagttacgga ttaaaacagt ttatatacga tgttcaggat ggtcagcaga
    10921 ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag agacggcgct
    10981 ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg tgcgcccatg
    11041 gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt ggagcccatg
    11101 atgaagatca tgctgatagt gttcagttta cccagcgata ctttagtttt gtcgccttta
    11161 taaccaaact tcaggaccag gccattcaga ccttccagcg ttcccagata gtgaccgaag
    11221 aaagatttga agatagccac gagtgcgatg atggaagccg catattccag tgtaatcgcg
    11281 aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga aagcactgga
    11341 atattctgcg ctttggcttc cgccatgttg gccggagaca gcgtaaacag gcagctaaag
    11401 gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat ttgggaacat
    11461 ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt agaaaccacg
    11521 aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat ccccagccag
    11581 acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc aacctggtcg
    11641 ataactgcag agttccagta agggatcagc gacaaagaaa tcagcaccag gctggcgata
    11701 aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa ccagatgacg
    11761 aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg tgcaaagccg
    11821 agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata aatccacagc
    11881 agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt tttaccaaaa
    11941 tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag acacagacgc
    12001 gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat cagaagaatc
    12061 gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc gccgattgcc
    12121 gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga ttgttttgtc
    12181 tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat taattaagcg
    12241 tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc ggaaataata
    12301 ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa tgcagcacat
    12361 gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc aatcatactg
    12421 tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg aacgatttcg
    12481 taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc ggtagttcgg
    12541 tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt ttcagactgt
    12601 acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat accagcaatt
    12661 aaaccgccac caccaattgg cacaatcacg ttatcgacat catagagatc ttccataatt
    12721 tccaggccaa tcgttccctg accagcaatc actttcggat catcgtaagg tgggataaaa
    12781 atacggcctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt gaagttatca
    12841 ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga tttcggcgcg
    12901 ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag cgaaacccct
    12961 tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc atcagtcagt
    13021 gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg catattttca
    13081 aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg aggcatgcct
    13141 gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatgtcatc aatagcaacc
    13201 ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat atgtaggtaa
    13261 ccgtaaccga cacctgcaag acagacaggt ggattattta tgtttaaata aaaacaaata
    13321 actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa tactctttgg
    13381 ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac cataccgcgg
    13441 catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga ttagaaccaa
    13501 aaggagacgt catatcacaa ggaattacag ttaagaaatc agcattgaga acaagattat
    13561 aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca tttctttgta
    13621 acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc cactgttcgt
    13681 tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg gctaccagca
    13741 caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt tctgcgctta
    13801 acgtaccaat cgcaaaatca agtcgaccgt cgcggattgc cggtaagaac gaagacagtt
    13861 gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc
    13921 ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc accaccgcct
    13981 cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta atggattcgg
    14041 aacgagagag taacaattga ccggcaggtg ttaatgttac tccggtattt ttccgcacca
    14101 ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg gccggttgag
    14161 ttaaccctaa ttcttttgca gccgagccga tagaaccact tctaatgact tcctgaaaga
    14221 ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac gttaattacc
    14281 tcattgacgg catgaagtgt atcaaaatga aatgaacagg atatgtgcga ccactcacaa
    14341 attaactttc aatactttcc agggtatcgt catgtcaatt aaataccttt aa
//
LOCUS       DAZFTN010000118        14358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFTN010000118 DAZFTN010000000
VERSION     DAZFTN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            159..2300
                     /locus_tag="ACHKV2_003987"
     CDS             159..2300
                     /locus_tag="ACHKV2_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO6941780.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2364..4256)
                     /gene="parE"
                     /locus_tag="ACHKV2_003988"
     CDS             complement(2364..4256)
                     /gene="parE"
                     /locus_tag="ACHKV2_003988"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO6941781.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4285..4866)
                     /gene="yqiA"
                     /locus_tag="ACHKV2_003989"
     CDS             complement(4285..4866)
                     /gene="yqiA"
                     /locus_tag="ACHKV2_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO6941782.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4866..5693)
                     /gene="cpdA"
                     /locus_tag="ACHKV2_003990"
     CDS             complement(4866..5693)
                     /gene="cpdA"
                     /locus_tag="ACHKV2_003990"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO6941783.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5718..6140)
                     /locus_tag="ACHKV2_003991"
     CDS             complement(5718..6140)
                     /locus_tag="ACHKV2_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO6941784.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6141..6770)
                     /gene="nudF"
                     /locus_tag="ACHKV2_003992"
     CDS             complement(6141..6770)
                     /gene="nudF"
                     /locus_tag="ACHKV2_003992"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO6941785.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6975..8456
                     /gene="tolC"
                     /locus_tag="ACHKV2_003993"
     CDS             6975..8456
                     /gene="tolC"
                     /locus_tag="ACHKV2_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO6941786.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8604..9275
                     /locus_tag="ACHKV2_003994"
     CDS             8604..9275
                     /locus_tag="ACHKV2_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO6941787.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9281..10441
                     /locus_tag="ACHKV2_003995"
     CDS             9281..10441
                     /locus_tag="ACHKV2_003995"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6941788.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSIYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10479..11267)
                     /gene="ygiD"
                     /locus_tag="ACHKV2_003996"
     CDS             complement(10479..11267)
                     /gene="ygiD"
                     /locus_tag="ACHKV2_003996"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO6941789.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11410..12183
                     /gene="zupT"
                     /locus_tag="ACHKV2_003997"
     CDS             11410..12183
                     /gene="zupT"
                     /locus_tag="ACHKV2_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO6941790.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12241..12411)
                     /gene="yqiD"
                     /locus_tag="ACHKV2_003998"
     CDS             complement(12241..12411)
                     /gene="yqiD"
                     /locus_tag="ACHKV2_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO6941791.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12673..13326)
                     /gene="ribB"
                     /locus_tag="ACHKV2_003999"
     CDS             complement(12673..13326)
                     /gene="ribB"
                     /locus_tag="ACHKV2_003999"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO6941792.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13430..13578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13700..13999
                     /gene="ubiK"
                     /locus_tag="ACHKV2_004000"
     CDS             13700..13999
                     /gene="ubiK"
                     /locus_tag="ACHKV2_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO6941793.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14034..14234)
                     /gene="glgS"
                     /locus_tag="ACHKV2_004001"
     CDS             complement(14034..14234)
                     /gene="glgS"
                     /locus_tag="ACHKV2_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO6941794.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         3654 a         3787 c         3490 g         3427 t
ORIGIN      
        1 attttttatt atttctcatt tactactaca ttaccgcatg attgaatgat tctcaattac
       61 atatccgatg gtaaaataac aaacggatag tgcttagtga gcaacaagaa acaattttca
      121 ctcacgacaa tccgtgagtt tttgggataa cagactaaat ggctaagttc acaccttcat
      181 tctcaggaat caaaggtcgg gcgctctttt cactgctctt tgcagcacca atgattcatg
      241 caaccgacac tgcaacgacc aaagatggcg aaacaatcac tgttacagcg gatgcaaata
      301 ccgcaactga ggcgaccgat ggttatcaac ctctgagcac ttccacggcg acattaaccg
      361 atatgccgat gctggatatc ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa
      421 atcagaatgc aacgacgctg gatgaagcgc tttataacgt cagtaacgtg gtacagacca
      481 atacattagg cggaactcag gacgcctttg tacgtcgtgg ttttggtgct aaccgggatg
      541 gctccatcat gaccaacggc ctgcgaactg tacttcctcg cagtttcaac gccgccacag
      601 aacgtgtgga agtgctaaaa ggtccggcct ccacgctgta tggcattctc gatcctggtg
      661 gattgattaa cgtcgtgacc aagcgcccgg aaaaaacatt ccatggttct gtctcagcca
      721 cgtcctccag ttttggcgga ggcactgggc aacttgatat cacaggtccc attgaaggca
      781 ctcagctggc ataccgcctg acgggggaag tgcaggatga agattactgg cgaaacttcg
      841 gtaaagagcg cagtacattt attgccccgt cactcacctg gtttggtgat aatgcaacag
      901 taaccatgct ctattcccat cgggactata aaactccgtt cgatcgtgga acgattttcg
      961 accttacgac gaaacagccc gtaaacgttg atcgaaaaat acgttttgac gaaccgttta
     1021 atattacaga tggtcagtcc gatctggcgc aactcaacgc agaatatcat ctcaatagcc
     1081 agtggacagc gcgctttgat tacagctaca gccaggataa atacagcgac aatcaggctc
     1141 gcgttaccgc gtatgatgca acgacaggaa cgctgacacg gcgtgttgat gcaactcagg
     1201 gatctaccca gcgtatgcat tctactcgtg cggatctgca agggaatgtt gatattgctg
     1261 ggttctataa tgagattctg ggtggggtgt catatgaata ttatgatctt ctgcgcacag
     1321 atatgattcg ctgtaaaaac gctaaagatt tcaatatcta caaccccgtt tatggcaata
     1381 ccagcaaatg cacaacggtt tcggcgtcgg acagtgatca gacgatcaaa caggagagct
     1441 actcagctta tgcacaggat gcgctctatc tgaccgataa ctggattgcc gtcgccggga
     1501 tccgctatca gtattacacg cagtatgcgg gtaaaggccg tccttttaat gtcaatactg
     1561 acagccgcga tgaacaatgg acgcccaaac tggggttagt ctacaaactg acgccatcgg
     1621 tatccttatt tgctaattat tcgcaaacat ttatgccgca atcgtcaatt gccagctaca
     1681 tcggagatct tccaccggaa tcatctaatg cttacgaagt cggggcaaaa ttcgagctgt
     1741 tcgatggtat aaccgcagat attgcgctgt ttgatatcca taaacgtaac gtgttgtata
     1801 ccgaaagtat tggtgatgaa accatcgcca aaacggcagg ccgcgttcgt tcaagagggg
     1861 tagaagtcga ccttgcggga gcattaactg aaaacattaa tatcattgcc agctacggct
     1921 ataccgatgc taaggttctg gaagatcctg attatgcagg gaaaccattg ccgaatgttc
     1981 ctcgtcatac cggttcgcta ttcctgacct atgacattca taacatgcca ggcaataaca
     2041 cactgacgtt tggcggtggt ggacatggcg taagccgtcg ttcggcaacc aatggggctg
     2101 actattatct gccaggctat ttcgttgccg atgccttcgc cgcatacaaa atgaaattgc
     2161 agtatccggt cacactgcaa ttaaacgtca aaaacctgtt tgataaaacg tattacacct
     2221 cttccatcgc cacaaataat ctggggaatc agattggcga tccgcgtgaa gtgcaattca
     2281 cggtgaaaat ggaattttga tcaaaaaagc ccggtataca cttgtatacc gggctatgtt
     2341 ttattcttct ggcgggagga ttatcagacc tcaatctccg ccatgtcgcc tttctcttgc
     2401 aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca gcatatccat catcgcgtca
     2461 gtacgctgat cgtcttcatc atcgatagtc agctgcacca gacggcgagt gttcggatca
     2521 agcgtggttt cgcgcaattg catcgggttc atttccccca gacctttaaa acgctggacg
     2581 ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc cctctttctc ttcttccgtc
     2641 agcgcgtaat aaacctcttt cccgagatca atacggtaga gcggtggcag tgcgacgtaa
     2701 acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga acaaagcgca gagcagtgta
     2761 gcaatgtgca aaccatcaga gtccgcatcc gcgaggatac agattttgcc ataacgaagc
     2821 tggctcagat cgtcgctgtc aggatcgata ccgatcgcta ccgaaatatc atgcacttcc
     2881 tgcgaggcca gcacttcgtc ggaagaaact tcccaggtgt taaggatctt acctttcagc
     2941 ggcatgatcg cctgatattc gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca
     3001 ccttccacga ggaacagttc ggtacggtta aggtcctgcg cggtacaatc agccagtttg
     3061 ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca cttttttagc cgcacgcata
     3121 cgacgctggg cgctggaaat cgccatctct gccagcaact ccgccgcctg aacgttctgg
     3181 ttcagccaca ggatgaaggc atctttcacc acgcccgaaa cgaatgccgc acactgacgc
     3241 gaagagagac gctctttggt ctgcccggca aactgtggat cctgcatttt tactgacagc
     3301 acataggcgc agcgatccca gatatcttcc gccgacaact ttacaccgcg cggcaaaatg
     3361 ttgcggtatt cacagaactc acgcatcgcg tccagcaggc cctgacgcag gccattaaca
     3421 tgggtaccgc cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag cagttcaccg
     3481 ccttccggca gccacagtag cgcccagtcc accgcttcag tatcgccagc gaaattaccg
     3541 ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc
     3601 agaccgtcct gatagcacca gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc
     3661 tcaacgccgg ggcacagtac tgctttggct ttcagcacat gcgtcaggcg tgaaacagaa
     3721 aaacgcgggc tatcgaagaa ggtttcatcc ggccagaaat ggacgctggt gccggtattg
     3781 cgtttaccgc aattgccgac aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg
     3841 atgttataaa cctgaccatc gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta
     3901 accaccgaaa tccccacgcc atgcaggccg ccagagaact ggtaattttt gttagagaat
     3961 ttaccgcctg catgcagacg gcaaagaatc agttcaaccg ccggtacacc ctcttccggg
     4021 tgaatatcca ccggcatccc gcgcccatcg tcaataactt ctaacgactg gtcagcatgt
     4081 aaaataacgt ccacgcgttt tgcgtgaccc gccagtgctt catccacact gttatcaatg
     4141 acttcttgcc ccaaatggtt agggcgagtg gtatcggtat acatccccgg acggcggcga
     4201 accggctcaa gcccggtgag tacctcaatg gcatcagcgt tataagtttg cgtcatggtt
     4261 taagttagca attcgagttg atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg
     4321 gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga cagtctggcg
     4381 gcaggaagcg tagtacgcca ccgcctggcg gtaatccagc acttcatctc ccgtctgttg
     4441 cagcagccag atcaaatccg gcgcttccag cgggtcaatc tgcatgactt taagatcgta
     4501 aatatggcgt gactctagca catattgctg cccggtgtag gggttctcgt tctgaccgag
     4561 atagtccgtc agcagttcaa acgggcgcac cgccgggttt accaccactg cgggcagcat
     4621 aaaacattgt gacaaccagg tggcgtaata tccccccagt gacgaaccga caatacccag
     4681 cgaatcgccg ccatgttcca ggacaatgga ttccagcagc tctgccgcgt cggaaggata
     4741 cggcggcaac tgcggaatga tcatctcaac gtcagggtga tgttccgcca gccagttttt
     4801 taacaagctc gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt
     4861 agacatcagt agccttctga agcggtatca ggttggaaac gtgtgtccgc caggcgatgc
     4921 acctcggtgg tcagcgtgcc atcagcatgt aactcgagag tacgccagcc gggcgcgata
     4981 gtatccagcg taaagttgga acagtgcggc ttaaactgca cacaggtcga cggtgttgcc
     5041 agcaggcggc gaccgttcca gtcgagatcc agctcctgat gaatatgacc gcacagcaag
     5101 tatttgacgt gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg
     5161 tgttgatcaa gccaactaca acccgcaggt agcggatgat gatgcagcag cagcaacgta
     5221 tggcgctctg gcgcatcagc cagtttacgt tccagccact caagctgaaa ctcgcttagc
     5281 tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca ggatttgcca ttgctcacca
     5341 ataaacaccc gcttcgccgg ggagataccc gcatcctgta acgcgctgta catcgcgggc
     5401 tggaaatcgt ggttgcccgg cagccagacg cagggcgcac gaaaacttgc gatgccttca
     5461 gcgaaatgct gataggccgc agaggattga tcctgcgcta aatcacctgt cgcgacaatc
     5521 aggtcgaatt cgtgctggtg tggacgaatc gcctccagca ccgcctggta gctttcccag
     5581 gtgtttaccc ctaagagggc ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt
     5641 aaaatcctga ctctggcctc accagccaga ggaagggtta acaggctttc caaatggtgt
     5701 ccttaggttt cacgacgcta ataaaccgga atcgccatcg ctccatgtgc taaacagtat
     5761 cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt
     5821 ttattaggat aatcataccg cgctttgaag cgaaaaatct gctggcttga acacacttca
     5881 gccaccatcg cgtcatgata cagacgcacc gtcattgacg gaaggctcca gtaactgatc
     5941 gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc
     6001 agccgatatt gtgcgtttgc cacctgatag cttacagttt cgccgggtgc gtcattgcgc
     6061 ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag
     6121 tcaggtgtgt aacgcttcat ttatgcccac tcatttttta acgcttgatg atgcagctgc
     6181 agccattgca aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc tacccactgg
     6241 tatgcctgtt cccggcttac cacatgaacg cgaatatctt cgttttcatc agccagaccg
     6301 tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca taattgacga acgctcactg
     6361 gtgccccccg ggcttgccag gaaacttaac accggtttgg tccgtttgac tatcagcccc
     6421 gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac tttcaccctc ttcaatcatt
     6481 ccggcaacca tctccagtag ccaaggggtt tcgctagtgt cgtatgcggc aattcgaatc
     6541 tgctcaatca gcacaacttc atcacgcact gggtcaaagg gtagcaagac tgcggcgtga
     6601 ccgcgctcaa aaatttcccg ccgtacctca tgactcattt gcccgttgaa tagacgatga
     6661 cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt
     6721 tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc
     6781 ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt
     6841 catgtgttaa attgaggcac attaacgccc tatggcacgt aacgccaacc ttttgcggta
     6901 gcggcttctg ctagaatccg caataatttt acagtttgat cgcgctaaat actgcttcac
     6961 cacaaggaat gcaaatgaag aaattgctcc ccattcttat cggcctgagc ctttctgggt
     7021 tcagttcgtt gagccaggcc gagaacctga tgcaagttta tcagcaagca cgccttagta
     7081 acccggaatt gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag
     7141 cgcgcagtcc attactgcca cagctaggtt taggtgcaga ttacacctat agcaacggct
     7201 accgcgacgc gaacggcatc aactctaacg cgaccagtgc gtccctgcag ttaactcaat
     7261 ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca gcagggattc
     7321 aggacgtcac gtatcagacc gatcagcaaa ccttgatcct caacaccgcg accgcttatt
     7381 tcaacgtgtt gaatgctatt gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct
     7441 accgtcaatt agatcaaacc acccaacgtt ttaacgtggg cctggtagcg atcaccgacg
     7501 tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa cgaagtgacc gcacgtaata
     7561 accttgataa cgcggtagag cagctgcgcc agatcaccgg taactactat ccggaactgg
     7621 cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac gcgctgctga
     7681 aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg cttgagccag gacctggcgc
     7741 gcgagcaaat tcgccaggcg caggatggtc acttaccgac tctggattta acggcttcta
     7801 gcgggatttc tgacacctct tatagcggtt cgaaaacccg tggtgccgct ggtacccagt
     7861 atgacgatag caatatgggc cagaacaaag ttggcctgag cttctcgctg ccgatttatc
     7921 agggcggaat ggttaactcg caggtgaaac aggcacagta caactttgtt ggtgccagcg
     7981 agcaactgga aagcgcgcat cgtagcgtcg tgcagaccgt acgttcctct ttcaacaaca
     8041 ttaatgcttc tatcagtagt attaacgcct acaaacaagc cgtagtttcc gctcaaagct
     8101 cattagacgc gatggaagcg ggctactcgg tcggtacgcg taccattgtt gatgtgttgg
     8161 atgcgaccac cacgctgtac aacgccaagc aagagctggc gaatgcgcgt tataactacc
     8221 tgattaatca gctgaatatt aagtcagccc tgggtacgtt gaacgagcag gatctgctgg
     8281 cactgaacaa tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa
     8341 cgccggaaca gaatgctatt gctgatggtt atgcgcctga tagcccggca cccgtcgttc
     8401 agcaaacatc cgcacgcact accaccagta acggtcataa ccctttccgt aactgatgac
     8461 gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc
     8521 gcattcttcc cccttctcgc ttcaatttcg accagccatc ctctattctg atgggtattt
     8581 accacaggtc ccggaagaca aaaatgaaac ggacaaaatc catacgccac gcatcgttcc
     8641 gcaaaaactg gagcgcacgc catctgacac cagtcgctct cgcggttgcc actgttttta
     8701 tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat gctgacgact
     8761 gttcagctgc aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga
     8821 aagaagccga acgtactgcg ccgaaatacg ccacccgtga agactgtgtt gctgaatttg
     8881 gtgaaggtca gtgccagcag gcaccagccc aggctggcat ggcaccagaa aaccaggcgc
     8941 aggcccagca atccagcggg agtttctgga tgccgctgat ggccggttac atgatgggtc
     9001 gtctgatggg cggcggtgcg ggatttgcac agcagccgct gttctcctcg aaaaacccag
     9061 ccagcccggc ttacggtaaa tataccgacg cgacgggtaa aaactatggc gcagcccagc
     9121 caggccgcac catgaccgta ccgaagacag ccatggcacc aaaaccggcg accaccacta
     9181 ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg
     9241 caaccggtac ctcttctcgt tcaatgggtg gctgataccg atggaaagag tcagtattac
     9301 cgagcgcccg gactggcgtg agaaagccca cgaatacggt ttcaattttc acaccatgta
     9361 cggcgagccg tactggtgtg aagatgctta ctacaagttg accctcgccc aggttgaaaa
     9421 gctggaagaa gtcaccgccg aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat
     9481 cgccagcgat gagctgatga ccaaattccg cattccaaaa cacacctgga gttttgtgcg
     9541 ccagtcatgg ctgacgcacc agccatctat ttattcgcgt cttgatctgg cgtgggatgg
     9601 cactggcgaa cctaaacttc tggaaaataa cgccgatacg ccaacgtcac tatacgaggc
     9661 ggcgttcttt cagtggatct ggctggaaga tcagcttaac gccggtaact tgccggaggg
     9721 cagcgaccag tttaacagtc tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga
     9781 acagtatggc ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg
     9841 taccattcag tatttgcagg actgcgcgac ggaagctgaa atcgctaccg agttcctcta
     9901 cattgacgat atcgggttag gtgaaaaagg tcagttcacg gatttacagg atcaggtaat
     9961 ttccaacctg ttcaagctgt atccgtggga atttatgttg cgtgagatgt tttccaccaa
    10021 gctggaggat gcaggcgtac gctggctgga accggcgtgg aagagcatta tctccaacaa
    10081 ggcacttcta ccgctactgt gggagatgtt cccgaatcac ccgaacctgc tgcccgctta
    10141 ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg
    10201 tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc attgaagcag cggaaggtcc
    10261 gtatggcgaa gaagggatga ttgttcagca attccacccg ttaccgaaat tcggcgacag
    10321 ctatatgctg attggtagct ggctggtgaa cgatcaaccc gccggaattg gcattcgtga
    10381 agaccgtgca ttgatcaccc aggatatgtc tcggttttat ccacatattt ttgttgaata
    10441 atcaatgatg ccggatggca ttcgccatcc ggtaattgtt aacctatctg caccgacagc
    10501 atacttaaac tgcccatttc tataccatcg accggaatgg taattggctc ctgcccatcc
    10561 cacgcaccta agacatacag caacggcaga tagtgctctg gcgttgggtt cgataacgcg
    10621 ccaccttcat ggtcgagata attcaccaga ggatgctgct ctaccggccc ttgccacgtc
    10681 agattcgctt tcacatactc attaaacgac atcgcccaag gatacggtga actatcaccg
    10741 tgccacttca ctgtacgcag gttatgcacc acgttgccgc tggcgaccaa cattattcct
    10801 tcatcacgca gcgccgcgag ttttcgcccc atttcgaaat gccaggcggc aggtttgcta
    10861 ctgtcgatac tcaactgcac catcgggata tccgcgtcag gatacatctt aatcagcacg
    10921 ccccaggaac cgtggtcaaa gccccaggct tctttatcca gcgtcaccgg gatcggcgct
    10981 aacagctcaa ccagacgctg tgccagcgca ggcgaacccg gagcaggata atgcgtatcg
    11041 tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc
    11101 actcctgttc cacgggtaaa ccagtgagcc gaaactacca caatcgcttg cgggcgtggc
    11161 aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat tatcttccag cacgttcatc
    11221 ggactaccgt gacctaaaaa caatgctggc atacgtgttg aagacatgat gatatcctta
    11281 actaaaggtg tcattttgat atcctcacga tacgcttgtt cggcggagta agaacccgga
    11341 taacaatgat gatgatcatc agttattttg acgatctgcc tgaaggtgaa gatttataag
    11401 gagttgtcga tgtcagtacc tctcattctg accatactgg cgggggcagc cacgtttatt
    11461 ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta
    11521 ggatttgcgg cggggatcat gttgctcatc tcattaatgg aaatgcttcc tgccgcacta
    11581 gcagctgaag gaatgtcgcc tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc
    11641 tattttggcc tggaccgcat gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg
    11701 gtgcagccgt tgccaaaatc gatcaagcgc acagccattc tgctcactct cggcatcagt
    11761 ctgcataact tcccggaagg gattgccacc tttgtcacgg cgagcagcaa cctggagctg
    11821 ggatttggca tcgcactggc cgtcgcgttg cacaatattc ctgaaggtct ggcggttgca
    11881 ggcccggttt atgcagcaac gggttctaaa cgcacggcaa ttctgtgggc ggggatttct
    11941 ggactggcag aaattcttgg tggtgtgctg gcgtggttaa tcctcggtag catgatttcc
    12001 ccggtggtca tggcagcaat catggcggcg gtcgcaggaa ttatggtggc gctctcggtt
    12061 gatgaattaa tgccgctcgc caaagagatt gaccctaata ataaccccag ctatggcgta
    12121 ctatgtggaa tgtcagtgat gggattcagt ttggtgctgc tacaaacggc gggaattggt
    12181 taaaagcaac atcagaagtt gtccagcgca gggacaactt ctgctagttt cactaaagcc
    12241 ttattctacc ttttcggctg tctcttctct cgtactgttt aagtatttgt tccgcgcttc
    12301 aagcagtgcg tctctgtcct ggcgaaatgc ccgacaataa cgtttcaaat gcaggaaata
    12361 acccaccaca accagaacaa tcaatacaat ccagtaccag gcgataaaca tccttttacc
    12421 cttaatattt aacaatacta aacatttagc gcataaattt cacatatcct ttttcggata
    12481 tattcatatg gtcgcataat aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa
    12541 tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagt
    12601 agggaaaaat aaaaaccgaa gcctgctaca acttcggttt cttgattaag gcagtaaatt
    12661 aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc
    12721 aatagtcacg agcgccatgt tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc
    12781 catcgtgcca tcgtcattag tcagctcaca cagcacacca gccggtttga agcctgccag
    12841 cgtcattaga tcaatagttg cttcagtatg accgccacgc gtcagtacac caccagcctg
    12901 agcgcggagt gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc
    12961 aatcgctgcg cgaacggtcg taatacggtc agcggcagaa acaccggtag tcacaccttc
    13021 agccgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat
    13081 cattggcaga tcgagttgtt tacggcgatc ttcagtaatg cacaggcaaa caataccact
    13141 accgtggcga atggtcagcg ccatctgctc aacagtcatg gtttctgccg ggaagatcat
    13201 atcaccttcg ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag
    13261 cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg
    13321 attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca gtcttaggag
    13381 tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc gttactctct
    13441 cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc ctttgagttc
    13501 gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg ggaatttcgc
    13561 cccgccctga gaataagcgg attcactata acgctaatga ttagcggcag caacgcatag
    13621 cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggcac tcaacaccta
    13681 tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt
    13741 tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga aaaaaatccg
    13801 ccaaacccta caagcgcagc tgacgcgtct cgatctggta agccgcgaag aattcgacgt
    13861 tcagacgcaa gtcctgttgc ggactcgtga aaaactggcg ttactggagc agcgcagcag
    13921 tgaactggaa gcgcgcaata attcggtagc agatcttcaa tctcctccgg ctatcccgcc
    13981 tattgataaa gcagaataaa gcaacacaaa aacgggccag acggcccgta atatcagtgt
    14041 tctaattcaa gttctctggt ttgcatcatc tgttggcaat accaggcata acgtgcgcaa
    14101 aaccaggttc tatgttcttc atccatgtta ccagtaacgg ccagaatatc gaccgggcga
    14161 ccttctgcgt gcattctggc aaaactacgc gctaggaaat cgaaattatt taaagaatta
    14221 agactatgat ccataagcac atcctccggt tatcgcatac cttttaaggc catatgcttt
    14281 ttctgatttc agtattcccc aatcagtgta gtgcctttaa acataacgtg cgtaaatata
    14341 ttgtccccga tcacactt
//
LOCUS       DAZFTN010000119        14323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFTN010000119 DAZFTN010000000
VERSION     DAZFTN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            246..1817
                     /gene="garD"
                     /locus_tag="ACHKV2_004002"
     CDS             246..1817
                     /gene="garD"
                     /locus_tag="ACHKV2_004002"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO6941795.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1966..2301
                     /gene="prlF"
                     /locus_tag="ACHKV2_004003"
     CDS             1966..2301
                     /gene="prlF"
                     /locus_tag="ACHKV2_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO6941796.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2301..2765
                     /gene="yhaV"
                     /locus_tag="ACHKV2_004004"
     CDS             2301..2765
                     /gene="yhaV"
                     /locus_tag="ACHKV2_004004"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO6941797.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2820..3629)
                     /gene="agaR"
                     /locus_tag="ACHKV2_004005"
     CDS             complement(2820..3629)
                     /gene="agaR"
                     /locus_tag="ACHKV2_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO6941798.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3878..5158
                     /gene="kbaZ"
                     /locus_tag="ACHKV2_004006"
     CDS             3878..5158
                     /gene="kbaZ"
                     /locus_tag="ACHKV2_004006"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO6941799.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5181..5654
                     /gene="agaV"
                     /locus_tag="ACHKV2_004007"
     CDS             5181..5654
                     /gene="agaV"
                     /locus_tag="ACHKV2_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO6941800.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5665..6444
                     /gene="agaW"
                     /locus_tag="ACHKV2_004008"
     CDS             5665..6444
                     /gene="agaW"
                     /locus_tag="ACHKV2_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO6941801.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6434..7312
                     /gene="agaE"
                     /locus_tag="ACHKV2_004009"
     CDS             6434..7312
                     /gene="agaE"
                     /locus_tag="ACHKV2_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO6941802.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7330..7764
                     /gene="agaF"
                     /locus_tag="ACHKV2_004010"
     CDS             7330..7764
                     /gene="agaF"
                     /locus_tag="ACHKV2_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO6941803.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7761..8894
                     /gene="nagA"
                     /locus_tag="ACHKV2_004011"
     CDS             7761..8894
                     /gene="nagA"
                     /locus_tag="ACHKV2_004011"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6941804.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9245..10399
                     /gene="agaS"
                     /locus_tag="ACHKV2_004012"
     CDS             9245..10399
                     /gene="agaS"
                     /locus_tag="ACHKV2_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO6941805.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10412..11272
                     /gene="kbaY"
                     /locus_tag="ACHKV2_004013"
     CDS             10412..11272
                     /gene="kbaY"
                     /locus_tag="ACHKV2_004013"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO6941806.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11439..11915
                     /gene="agaB"
                     /locus_tag="ACHKV2_004014"
     CDS             11439..11915
                     /gene="agaB"
                     /locus_tag="ACHKV2_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO6941807.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11987..12757
                     /gene="agaC"
                     /locus_tag="ACHKV2_004015"
     CDS             11987..12757
                     /gene="agaC"
                     /locus_tag="ACHKV2_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO6941808.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12747..13538
                     /gene="agaD"
                     /locus_tag="ACHKV2_004016"
     CDS             12747..13538
                     /gene="agaD"
                     /locus_tag="ACHKV2_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO6941809.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13539..14294
                     /gene="agaI"
                     /locus_tag="ACHKV2_004017"
                     /pseudo
     CDS             13539..14294
                     /gene="agaI"
                     /locus_tag="ACHKV2_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         3454 a         3613 c         3743 g         3513 t
ORIGIN      
        1 tgcaaatgct aatttaatta atactattta aatattattt tgagcatatg cacataaggt
       61 tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcattgcaa tccctgaaat
      121 gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa
      181 gtcatttatt tttaaatgag accaggtcct cattttaata acctctggct ggagaatatt
      241 gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc
      301 acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt
      361 ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctcg
      421 acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg
      481 caatcccacg cggaagctgg atcgacgaat caatggttgt actgccgaaa gcgccgccgt
      541 tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca
      601 cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca
      661 ccaccagcgt ccactgtgtg gcaggcgtgg tggactacgt agtaaaaatc attgaacgcg
      721 atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt
      781 gtggcgtggc gattaacgca ccggcggcag ttgtacctat ccgtaccatt cacaatattt
      841 cgctgaatcc taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc
      901 agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta gaaagcgcca
      961 gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt
     1021 tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg
     1081 cttcagaact ggttgtcggc atgcagtgcg gtggcagcga tgccttttct ggcgtaacgg
     1141 caaacccggc ggttgggtat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt
     1201 tctcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg
     1261 aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg
     1321 gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaatg
     1381 tagtggagaa agcgctcggc tccattgcta aatcgggtaa aagtgcaatt gttgaagtgc
     1441 tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg
     1501 attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg
     1561 gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggcg acccgcaccg
     1621 agctggcgaa ccgctggttt gatttaatgg atattaacgc gggcactatc gccaccggcg
     1681 aagaaaccat tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg
     1741 ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc
     1801 cagcaccggt gacctgattt cttttggaac gtccctcgca aaacatggcc ttagtgccat
     1861 gtttttattg tttaaagccc ccacgtccat taataatgca tttgcattac cttagttcaa
     1921 gcttataatt tgagcagaaa acaggctgta aaaggacagt gaatcatgcc cgctaatgct
     1981 cgctctcacg ctgtactgac cactgaatca aaggtcacga tacgcggaca aacaactatc
     2041 cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc aggacagcat tcattacgaa
     2101 attctgcctg gtgggcaagt atttatgtgc cgactgggag atgagcagga ggatcatact
     2161 atgaatgcat ttttgcgttt tctggataca gatatccaga acaacccgca aaaaactcgt
     2221 ccattcaaca ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa cattgatgat
     2281 gagattggcg acgacgaata atggattttc cacaaagggt taatggttgg gcgctatatg
     2341 ctcatccctg ttttcaggaa acctacgacg ctttagttgc cgaagtcgag acattaaagg
     2401 gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa gttattggcg gtggtccata
     2461 aagtgattga ggagcatatc acggtcaatc catcatcacc ggcattccgt catggcaagt
     2521 cgttaggctc tgggaaaaat aaagactggt cacgggtaaa atttggtgct ggtcgctatc
     2581 gtctcttctt tcgttacagt gaaaaagaga aagtcatcat tctgggatgg atgaacgatg
     2641 aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt
     2701 taaaaagagg acatcctcct gccgactggg aaaccctcac ccgagagaca gaagaaaccc
     2761 attgatggtg ttcacaatgc ccgccgaacg cattcgtgtt gaccagcggg caccctttct
     2821 cactccccga ccagaatcac ttcaacccca gcctttcgca gcccttccag gctatccgca
     2881 ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa
     2941 ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac
     3001 atccggcggt ttaaacgggc ttcatcttca ttatgcgtgc tgacgccgcg ctccagatcg
     3061 atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc
     3121 tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca
     3181 acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg
     3241 tcagtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc
     3301 acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcattgcg
     3361 gtgttcagcg cgcttttatc ttccactgat ggctcgactg acggcgttgt gctatcgcag
     3421 atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatcg
     3481 ttgcggatcg tcacggtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc
     3541 ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc
     3601 ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat
     3661 ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattgac
     3721 gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca gatcagaaaa
     3781 ctattatctt tcgttttatt tttatctcac catgacgcag tatcaactga aacaaaacga
     3841 aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt
     3901 gagacagcac aaagcgggca aaacaaatgg aatttatgcc gtttgttccg cacatccgct
     3961 ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc
     4021 aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg
     4081 cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt tgattctggg
     4141 tggtgaccat ctggggccaa accgctggca aaacctgccg gccgctcagg caatggccaa
     4201 tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg
     4261 cagcatgtcc tgtcaggacg atccgattcc cttaactgat gacatcgtgg ctgaacgcgc
     4321 cgcccgtctg gcgaaagtgg cggaagaaac ctgtcttgaa cactttggcg aagccgatct
     4381 ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga
     4441 gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt
     4501 tgaaaagcaa ggtttgaatg ccatctggcc acgcatcatt gccctggtgg ttcaacccgg
     4561 cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga ccgccttaag
     4621 ccagatggtc gaaagctacg aaacgctgat tttcgaagcg cactctaccg attatcaaac
     4681 gccgcaatcg ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc
     4741 gctgaccttc gccctgcgtg aagctctgtt ctctctggca gcgattgaag aagaactggt
     4801 gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc tcaaccgccc
     4861 ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta
     4921 tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc
     4981 tcatctggta cgtaatctgg cggattcacc aattccgctg ccgctgatca gccagtatct
     5041 gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac gggaactcat
     5101 tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc
     5161 aaaacaaaga ggaacacgct atgccaaata ttgttttaag ccggattgat gaacgcttga
     5221 ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtcg
     5281 ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag
     5341 aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg
     5401 ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga
     5461 aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaagc
     5521 aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga
     5581 aagccgctgg ggtggaatgc ttcgttcagg gcatcccgac agagcctgct gtggacctct
     5641 ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt cgcgttgggc
     5701 attatcgcct ttatcgctgg cctggatatg tttaacggct taacccatat gcaccgccca
     5761 gtggtcctcg gcccgttagt cgggctggta cttggcgatc tgcataccgg tattttaacc
     5821 ggcggtacgc tggaactggt gtggatgggg ctggccccgc tggcgggcgc acagccgcct
     5881 aatgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc
     5941 gatgtcgcag tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc
     6001 ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat
     6061 actcgcggca ttgaacgcgt aaactatctg gcactgctgg cactcggcac tttctatttt
     6121 ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc
     6181 ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt
     6241 tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt catcctgggc
     6301 tttgttgccg cagcctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg
     6361 atggcgctga tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa
     6421 gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga
     6481 aacattgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg gcgcggagct
     6541 gacgaaaaaa gatatcaatc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa
     6601 ctacgaacgt atgcaggctt ccggctggtt gtacggtctg ctgcctgctc tgaaaaagat
     6661 ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac
     6721 ccatccgttt ctggtgacat ttgttatcgg cattatcctt gcgatggagc gttctaagca
     6781 ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg
     6841 cgatgcaatg ttctggctaa cgctactgcc gatttgtggc gggattggag ccagtctggc
     6901 attgcaaggc tccattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtccacct
     6961 ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattccact
     7021 cattaaagct aataccaaaa aagtcggcca tgctgcgtct atcgttggga tgacggtaat
     7081 cggtgcgctg gtggcaacct atgttcgttt aagcaccacg ctggaaatca cagcgggcga
     7141 cgcagtggtt aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgtt
     7201 ggtctacacc ctgaccatgt tttggttggt acgccgcggc tggagtccgc tgcgtctgat
     7261 tgcagttacc gtggttctcg gcatcgttgg taaattctgc catttccttt aaatacaatg
     7321 aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag cggcatggaa
     7381 aaagcaatga agcaaattct cggtgagcaa tcgcagttta tcgccatcga ttttccggaa
     7441 acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt
     7501 gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca
     7561 acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg gcaccaattt gcaactgcta
     7621 ctggagatgg tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg
     7681 gagtgtggac atcgtgggct gaccagtctg gtggacgagt taggccgctg ccatgaagaa
     7741 tgtccggtcg aggaagggat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag
     7801 agggatggct cgatgaccat cagttgcgta ttgctgacgg tgtcatcgca gcaatcgaac
     7861 cgattccagt gagcgtgact gaacgcgatg cggaactgct ctgccccgct tacatcgaca
     7921 cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca
     7981 agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttgccgact accgtaaccg
     8041 cgccgcttag taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg
     8101 gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata
     8161 aaggcgcgca tccgccggag ttgtttcgcg agcttgaaat tgccgagctg gatcaattga
     8221 ttgccgtttc tcagcacacc ttacgcgtgg tagcgctggc accggaaaaa gagggggcat
     8281 tgcaggccat ccgccatctt aaacagcaaa atgtacgagt gatgctgggg catagcgcgg
     8341 cgacctggca acaaactcgc gccgcgtttg atgctggtgc cgacggcctg gtgcattgct
     8401 ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg ggattaacgg
     8461 acaagcgcgc ctggctggaa ctgatagccg atggtcatca tgtgcatccg gcggcaatgt
     8521 cgctgtgttg ttgctgtgcg aaagagagaa tcgtactgat caccgacgcg atgcaggcag
     8581 ccgggatgcc ggatggtcgc tatacgttat gtggcgaaga agtgcagatg cacggtggcg
     8641 ttgtccgtac cgcgtccggt gggctggcgg gcagtacgct gtctgttgat gcggcagtgc
     8701 gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc cattcatatg gcgtcgctgc
     8761 atccggcgcg aatgctgggt gttgatggtg ttctgggatc gcttaaaccg ggcaaacgcg
     8821 ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca aatctggatt cagggtcaat
     8881 tagcttcgtt ttgatggttt gctccattat tgggccttca cttcccccgt aaggcctttc
     8941 tttttctttc gttttgatct gtgcagcggt gtcggatgcg acgctaacgc gtcttatccg
     9001 acctacagtt ggtgaccgca aggccggata aagcgtttgc gccgcatcca gcaatccctt
     9061 ttgcttcctt tatcttttct ttcaacgatc acggatttcg ttttatttcc tttttctcca
     9121 ttgaactttc ggtttctttt ctatagattt taatcaacga aagacatcac caagtgaaat
     9181 gaaacgaaag gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg
     9241 agttatgcca gaaaattaca cccctgctgc cgccgcaacc ggtacatgga ctgaagaaga
     9301 gatccgccat cagcctcgcg catggatccg ttcgctcacc aacatcgacg cgctacattc
     9361 cgcgcttaat aacttccttg aaccgttact gcgcaaagag aatctgcgga tcatcctgac
     9421 cggagccgga acctcggcat ttatcggtga catcatcgcg ccgtggctcg ccagccatac
     9481 cggtaaaaac ttcagcgccg taccgaccac cgatctggtc actaatccga tggactacct
     9541 gaacccagcc catccgctgc tgttgatctc cttcggtcga tccggcaaca gcccggaaag
     9601 cgtcgcagcc gtggaactgg caaatcaatt tgtaccggaa tgctatcacc tgccgatcac
     9661 ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg cgtttgccgt
     9721 gctgatgccc gcagaaacgc acgatcgcgg ctttgcgatg accagcagca ttaccaccat
     9781 gatggccagc tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa ccttccgcga
     9841 cgtggcggat cgttgccagg cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt
     9901 tggttacgca ccgtggaaac ggatcgttta tctcggcagc ggtggcttac agggcgcagc
     9961 acgcgagtcg gcgctgaaag tgctggaact gacggcgggt aaactggcgg ccttttatga
    10021 ttctccaacc ggattccgtc atggaccaaa atcgctggtc gataacgaaa cactggtggt
    10081 ggtatttgtc tccagccacc cttacacccg tcagtatgat cttgatctgc tggctgaact
    10141 tcaccgtgac aaccaggcaa tgcgtgtaat cgccatcgcc gcggaaagca gcgacatcgt
    10201 cgctgccggt ccacatatca tcctgccacc gtcacgtcac tttatcgacg ttgagcaggc
    10261 attttgcttc ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc acatgggcaa
    10321 tacgccggat accccatcag ccagtggcac cgttaaccgc gtggtgcaag gcgtaatcat
    10381 tcatccgtgg caggcataag aggatcgcat tatgagcatt atctccacca aatatctgtt
    10441 acaggacgcc caggccaatg gctacgcggt gcctgctttt aacattcata acgccgagac
    10501 gatccaggcg atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac
    10561 gccggggacc tttaaacata tcgcgctgga agagatctac gccctgtgca gcgcctattc
    10621 cacaacctac aacatgccac tggcgctgca tctcgaccac cacgaatcgc tggatgatat
    10681 tcgccgtaaa gtccacgcag gtgtgcgcag tgcgatgatc gacggcagcc acttcccgtt
    10741 tgccgagaac gtaaagctgg tgaaatcggt tgtcgacttc tgccactccc aggattgcag
    10801 cgtggaagca gaactgggcc gcctgggcgg tgttgaagat gacatgagcg ttgacgccga
    10861 aagtgcattc ctgaccgatc cacaagaagc taaacgcttt gtcgaactga ctggcgtcga
    10921 cagcttggcg gtagcgattg gtacggcgca cggcttatac agcaaaacgc cgaagattga
    10981 tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc
    11041 cagcgatgtt ccggatgaat ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa
    11101 cgttgccaca gaattaaaaa tagccttcgc cggcgcggtt aaagcctggt ttgcggaaaa
    11161 tccgcagggt aatgatcctc gttattatat gcgcgtcgga atggatgcga tgaaagaagt
    11221 tgtcagaaat aaaattaatg tctgtggttc agcgaatcga atttcagcat aatcattagt
    11281 tttttatgat ttatcccaat gtacttcccg attaatatcg gggagtgcct taatggaaaa
    11341 ggagatgact aaaccttaat aaatagatca gtacaatatc gcaacaataa tatatttaaa
    11401 aaaattatat tattcaactt tatggtgagg attacacaat gagcagtcca aatattctct
    11461 taacccgtat tgataaccgt ctggttcacg gtcaggttgg cgtgacctgg acatccacca
    11521 tcggtgcgaa tctgctggta gtcgtggatg atgttgtcgc taacgatgat attcaacaga
    11581 aattaatggg tattaccgca gaaacctacg gctttggcat tcgtttcttt actatcgaaa
    11641 aaaccattaa cgtcatcggc aaagctgcac cgcatcagaa gatcttcctg atttgccgta
    11701 cgccacaaac ggtacgtaaa ttggtagaag gtggcattga cctgaaagat gtcaacgtcg
    11761 gcaatatgca tttctcggaa gggaaaaagc aaatcagcag taaagtttat gtcgatgacc
    11821 aggatctcac ggacttacgt tttattaaac aacgtggcgt gaatgttttc attcaggacg
    11881 tccctggcga tcaaaaagaa caaatccctg actaaatcta aaatcgcctt aatattggtt
    11941 tgaggtaata aaaatgcatg aaataaccct actttaggga ttatccctgg cggcgttagt
    12001 tttcgttctg gggattgatt tttggctgga agccttattt ttattccgcc cgataatcgt
    12061 ttgtacccta actggcgcta ttctcggtga tattcagact ggcttaatta ccggtggtct
    12121 gacagagttg gctttcgccg gattaacccc tgcaggtggt gttcagccgc ccaacccgat
    12181 tatggcgggt ctgatgacca ccgtcattgc atggtctacg ggcgttgatg ccaaaacggc
    12241 aattggtctt ggcctgccgt ttagtttgtt aatgcagtac gtcattctgt tcttctattc
    12301 tgctttctca ttatttatga ccaaagccga taaatgcgcg aaagaggcgg atacggcagc
    12361 gttttcccga cttaactgga caacgatgct catcgtcgct tcagcgtatg cggtgattgc
    12421 tttcctctgt acttacctgg cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc
    12481 ttggctgacc cacggctttg aagtggctgg cggtattctg cctgccgttg gttttggctt
    12541 gctgctgcgc gtgatgttca aagcacaata tatcccttac ctgatcgccg gcttcctgtt
    12601 tgtttgctac atccaggtca gcaacctgtt gccggttgcc gtactgggcg caggctttgc
    12661 ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag
    12721 taaaaatgaa gaagaggact acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc
    12781 gtctgggctt tcgttcgtcg ctgctgcaag cgagctttaa ctacgaaagg atgcaagcgg
    12841 gcggttttac ctgggcgatg ttgccgatcc tgaaaaagat ttataaggat gacaaaccgg
    12901 gcctaagcgc agcgatgaaa gataacctcg aatttattaa tacccacccg aatctggtcg
    12961 gattcctgat ggggttatta atttcgatgg aagaaaaagg agaaaaccgc gacaccatta
    13021 aaggcctcaa agtggcactg tttggcccaa tcgccgggat tggcgatgcg attttctggt
    13081 ttactttgtt gccgattatg gcgggaattt gctcatcatt tgccagccag ggaaacctgc
    13141 tggggccgat tctatttttc gccgtttacc tgcttatctt tttcctgcgc gtcggctgga
    13201 cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa agtgcgagag aactcgcaga
    13261 tgattgctcg ctcggcaacc atcctcggga tcacggtaat cggcgggctg atcgcttcgt
    13321 atgtgcatat taacgtggtg acatcgtttg ccatcgacag tacccacagc gtcgcgctgc
    13381 aacaggattt cttcgataaa gtcttcccga acattttacc gatggcctac accctgctga
    13441 tgtattactt cctgcgggtg aaaaaagcgc atccggtgct gttaatcggc gtgacttttg
    13501 tgctctctat tgtttgttcc gcattcggca ttttgtaaat ggaacgaggc actgcgtctg
    13561 gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac ccttcagcaa gttgaaaact
    13621 atacggcgtt aagtgaacgt gccagcgaat atttattggc cgtgatccgt agcaaaccgg
    13681 atgccgtgat ttgcctggcg accggagcca cgccattact gacgtatcat tatctggtag
    13741 aaaaaatcca ctagcagcag gttgatgtca gccagctcac cttcgtgaag ctcgacgaat
    13801 gggtggatct gccattaacg atgcctggta cctgcgaaac tttcctgcaa cagcatatcg
    13861 tgcagccgct ggggctacgt gaagaccagc tcatcagctt tcgctccgaa gagataaatg
    13921 agacagagtg cgaacgggta acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg
    13981 ttctcggatt ggggaaaaac ggtcatcttg ggctgaacga accgggagaa aacctgcaac
    14041 cggcctgcca tatcagtcaa cttgatgcca gaacacagca acatgagatg ttaaaaaccg
    14101 cgggtcgccc cgtgactcgt gggatcacct taggcctgaa ggatattctc aatgcccgcg
    14161 aggttttgtt actggtgact ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg
    14221 ctaaagtctc tacctctatc ccggcttcgt ttttgtggct gcacagtaac tttatttgtt
    14281 taattaatac ctaaccagat gaaaaatttg ctcaaaatgt tta
//
LOCUS       DAZFTN010000120        13676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFTN010000120 DAZFTN010000000
VERSION     DAZFTN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(144..1502)
                     /gene="plaP"
                     /locus_tag="ACHKV2_004018"
     CDS             complement(144..1502)
                     /gene="plaP"
                     /locus_tag="ACHKV2_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO6941810.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1492..1554)
                     /gene="yoeI"
                     /locus_tag="ACHKV2_004019"
     CDS             complement(1492..1554)
                     /gene="yoeI"
                     /locus_tag="ACHKV2_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO6941811.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1769..2698)
                     /gene="yeeY"
                     /locus_tag="ACHKV2_004020"
     CDS             complement(1769..2698)
                     /gene="yeeY"
                     /locus_tag="ACHKV2_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6941812.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2744..3568)
                     /gene="yeeZ"
                     /locus_tag="ACHKV2_004021"
     CDS             complement(2744..3568)
                     /gene="yeeZ"
                     /locus_tag="ACHKV2_004021"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6941813.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3994..4044
                     /gene="hisL"
                     /locus_tag="ACHKV2_004022"
     CDS             3994..4044
                     /gene="hisL"
                     /locus_tag="ACHKV2_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO6941814.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    4020..4143
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4190..5089
                     /gene="hisG"
                     /locus_tag="ACHKV2_004023"
     CDS             4190..5089
                     /gene="hisG"
                     /locus_tag="ACHKV2_004023"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO6941815.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5095..6399
                     /gene="hisD"
                     /locus_tag="ACHKV2_004024"
     CDS             5095..6399
                     /gene="hisD"
                     /locus_tag="ACHKV2_004024"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO6941816.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6396..7466
                     /gene="hisC"
                     /locus_tag="ACHKV2_004025"
     CDS             6396..7466
                     /gene="hisC"
                     /locus_tag="ACHKV2_004025"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO6941817.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7466..8533
                     /gene="hisB"
                     /locus_tag="ACHKV2_004026"
     CDS             7466..8533
                     /gene="hisB"
                     /locus_tag="ACHKV2_004026"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO6941818.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8533..9123
                     /gene="hisH"
                     /locus_tag="ACHKV2_004027"
     CDS             8533..9123
                     /gene="hisH"
                     /locus_tag="ACHKV2_004027"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO6941819.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9123..9860
                     /gene="hisA"
                     /locus_tag="ACHKV2_004028"
     CDS             9123..9860
                     /gene="hisA"
                     /locus_tag="ACHKV2_004028"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO6941820.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9842..10618
                     /gene="hisF"
                     /locus_tag="ACHKV2_004029"
     CDS             9842..10618
                     /gene="hisF"
                     /locus_tag="ACHKV2_004029"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO6941821.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10612..11223
                     /gene="hisIE"
                     /locus_tag="ACHKV2_004030"
     CDS             10612..11223
                     /gene="hisIE"
                     /locus_tag="ACHKV2_004030"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO6941822.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11320..12297)
                     /gene="wzzB"
                     /locus_tag="ACHKV2_004031"
     CDS             complement(11320..12297)
                     /gene="wzzB"
                     /locus_tag="ACHKV2_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO6941823.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12443..13609)
                     /gene="ugd"
                     /locus_tag="ACHKV2_004032"
     CDS             complement(12443..13609)
                     /gene="ugd"
                     /locus_tag="ACHKV2_004032"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6941824.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
BASE COUNT         3487 a         3529 c         3467 g         3193 t
ORIGIN      
        1 tattcatgac tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt
       61 attaatgagg atttcgataa aaaagaataa aaaaaccgga gccaaatgtt cattcgactc
      121 cggtttatta ttagaaatga tgattatgct acgtcttcgt actgcggaac cgggttacgg
      181 aagctcttgg tgacgcaagc caggtaaatc agaccgatag cagcccagat cagacccaga
      241 accattgagc tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc
      301 ggcaggaaca gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag
      361 aactgcgaga tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttgatc
      421 agcgccgtag ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg
      481 atgatgttca tcgccggagt acgccatttc gggtgtacat aaccgaagaa acttttcggg
      541 aacacgccgt cacgacccat tacgtacatc agacgcgcta cgcctgcgtg cgccgccata
      601 ccggacgcca gtacggtaat ggtggagaag atcagcgcac cgacctggaa cgctttaccc
      661 gcaacgtaca gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc
      721 gggaagtaca gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc gatcagcgcg
      781 gtcaggaaaa tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacagattg
      841 ctgatgccgt caaagccggt aaaggagaag cacaggattg tcgccccggt aatcatcggg
      901 ataacatgcg catcgccaga ccagaatgga cgagtgctcg ccagcgtacc agcgccttca
      961 ccttcaaata cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac
     1021 acgacgatta cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc
     1081 ataaaggcca ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca
     1141 aaatagattt tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa
     1201 gaccaaccca ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca
     1261 gagccagcag aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc
     1321 gcaatcaatg cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata
     1381 ccaaaagtat caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga
     1441 actaacgtaa gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac
     1501 atggtcattc tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt
     1561 ggattcctca gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg
     1621 gcctcttggt atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg
     1681 tttttagtca ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga
     1741 atgagaggct tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg
     1801 cataaacacc ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa
     1861 gtcctgcggt ctgttcattc cagccaatcc agacgggttc aaagagagtt tgggcgacca
     1921 ctttcttctc gattaacgcg ccactatcga gaaaacgttg cgccagataa cggggtaaat
     1981 agccgcagcc gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa
     2041 tggcttcttg ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc
     2101 ccaccacaat cgcccgatat cgtttaatga tacgacggtt taatggttct tcttcctggg
     2161 ctaacggatg atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac
     2221 caaactcact ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct
     2281 gagtcagggc atcccatgaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgt
     2341 tatgatgttg ataaaaagct tcaatgagtg gcgcaagcag agaaaaagga aacgtgtcgt
     2401 ccacgccaat caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca
     2461 gttctcgcac ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg
     2521 tgaatttagc gcggtgaccg ctacgatcaa gcaattgaat attaaggtcg ctttccagct
     2581 tgtgaacggt gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa
     2641 aactgccttc tttttctaag gcatcgagga tcatcaacac atccagcagt ggtttcatac
     2701 acgccccctt gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta
     2761 ccagcggatc gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa
     2821 taatcttacc tttgccgctg tccagactat ttctgaattg cggaggttcc agccccagta
     2881 aacgggccat ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat
     2941 agatgtgtcc gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta
     3001 aatggactaa attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc
     3061 cgggatgacg ccccggtccg accaggcccg caagacgcag aatatcgacc gaagtaccgg
     3121 gtaaattgtg cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat
     3181 tacgcggggt ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgaactgg
     3241 taaaaataat tcggggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt
     3301 gtaaatagaa ctcatcgccg gggccgctac gacgggccgg aagcgtaatg accagcgcat
     3361 cggcatccat cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa
     3421 gcagatagct atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac
     3481 tcccggtgac ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc
     3541 ctaacccgac aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa
     3601 ggctacgcta gcgtatcaaa actgacaatt cattctatga atgaaatcga aactgttagc
     3661 aaatatacca ttgcctgatg cgctccgctt attagtccta caaaacatca atatattgaa
     3721 tttaggcaac atttgtagac cagataaggc attcacgccg catccggcag gaacaaagcc
     3781 cactttagtt acagaacaca atgttcttga gggttttaat gacatatttt cacgattact
     3841 tttttaacct agtcactctt agccccctcc tttccccgct cattcattaa acaaatccat
     3901 cgccataaaa tatataaaaa agcccttgct ttctaacgtg aaagtggttt aggttaaaag
     3961 acatcagttg aataaacatt cacagagact tttatgacac gcgttcaatt taaacagcac
     4021 catcatcacc atcatcctga ctagtctttc aggcgatgtg tgctggaaga cattcagatc
     4081 ttccagtggt gcatgaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt
     4141 tattgcgcgg ttgataacgg ttcagacagg tttaaagagg aataagaaaa tgacagacaa
     4201 cactcgttta cgcatagcta tgcagaaatc cggccgttta agtgatgact ctcgcgaatt
     4261 gctggcgcgc tgtggcatta aaatcaatct tcacacccag cgcctgatcg cgatggcaga
     4321 aaacatgccg attgatattc tgcgcgtgcg tgacgacgac attcccggtc tggtaatgga
     4381 cggcgtggta gatcttggga ttatcggcga aaacgtgctg gaagaagagc tgcttaaccg
     4441 ccgcgcccag ggtgaagatc cacgctactt taccctgcgt cgtctggatt tcggcggctg
     4501 ccgtctttcg ctggcaacgc cggttgatga tgcctgggac ggcccgctct ccttaaacgg
     4561 taaacgtatc gccacctctt atcctcacct gctcaagcgt tatctcgacc agaaaggcat
     4621 ctcttttaaa tcctgcttac tgaacggttc tgttgaagtc gccccgcgtg ccggactggc
     4681 ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg gaagcaaacg gcctgcgcga
     4741 agtcgaagtt atctatcgct cgaaagcctg cctgatacaa cgcgatggcg aaatggaaga
     4801 atccaaacag caactgatcg acaagctgct gacccgtatt cagggtgtga tccaggcgcg
     4861 cgaatcaaaa tacatcatga tgcacgcacc gacccaacgt ctggatgaag tcatcgccct
     4921 gctgccaggt gccgaacgcc caaccattct gccgctggcg ggtgatcaac agcgcgtagc
     4981 gatgcacatg gtcagtagcg aaaccctgtt ctgggaaacg atggaaaaac tgaaagcgct
     5041 gggtgccagt tcaatcctgg tcctgccgat tgagaagatg atggagtgat cgccatgagc
     5101 tttaacacaa tcattgactg gaatagctgt actgcaaagc aacaacgcca gctgttaatg
     5161 cgcccggcga tctccgcttc tgaaagcatt acccgcactg ttaacgatat tctcgatagc
     5221 gtgaaagcac gcggtgatga cgccctgcgg gaatatagcg cgaagtttga taaaaccacg
     5281 gttaccgcac tgaaggtgtc tgctgaggaa attgccgccg ccagcgaacg cctgagcgac
     5341 gagctaaaac aggcgatggc ggtggcagta aagaatattg aaaccttcca cactgcgcaa
     5401 aaactgccgc cggtagatgt agaaacgcag ccaggcgtgc gttgccagca agtcacgcgc
     5461 ccggtagctt cagttgggtt gtatattcct ggcggctccg ccccgctctt ctcaacggta
     5521 ttaatgctgg caactccggc gcgtattgcg ggctgtaaaa aagtggtgtt gtgctcaccg
     5581 ccgccgattg ccgatgagat cctttatgcg gcgcagctgt gcggtgtgca ggacgtgttt
     5641 aacgtcggcg gcgcacaggc cattgccgcg ctggcgtttg gtacggaatc tgtgccgaaa
     5701 gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg aggcaaaacg tcaggtgagc
     5761 cagcgtctgg acggtgcggc gatcgatatg cccgcaggcc cgtcggaagt gttggtcatt
     5821 gccgacagcg gcgcaacgcc ggatttcgtg gcttctgatt tgctttctca ggctgaacac
     5881 ggcccggact cacaggtgat tttactgacg cctgacgctg atatggcgca tcaagttgcc
     5941 gaagccgtcg aacgccagtt agcagaactg ccgcgtgccg aaaccgcccg ccaggcactg
     6001 aacgccagcc gcctgatcgt gactaaagat ttagcgcagt gcgtggagat ctccaaccag
     6061 tacggcccgg agcacctgat cattcagacc cgcaacgccc gcgaactggt cgatggcatc
     6121 accagcgccg gttcggtatt tcttggtgac tggtcaccgg aatcggcagg tgattacgcc
     6181 tccggaacca accatgttct accgacttac ggctacaccg ccacctgttc cagcctcgga
     6241 ctggcggatt tccagaagcg gatgaccgtg caggaactgt cgaaagtagg tttctccgcg
     6301 ctggcttcga ccattgaaac actggccgcc gccgagcgcc tgaccgccca caaaaatgcc
     6361 gttactttgc gtgttaacgc ccttaaggag caagcatgag caccgtgact attaccgatt
     6421 tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc ggcgcgtcgt ctgggcggta
     6481 atggcgacgt ctggctgaac gccaacgaat atcccacagc cgtggagttt cagcttactc
     6541 agcaaacgct caaccgctac ccggaatgcc agccgaaagc ggtgattgaa aattacgcgc
     6601 aatatgcagg cgtaaaaccg gaacaggtgc tggtcagccg tggcgcggac gaaggtattg
     6661 aactactgat tcgcgctttt tgcgaaccgg gtaaagacgc catcctctac tgcccgccaa
     6721 cgtacggcat gtacagcgtc agcgctgaaa ccattggcgt cgagtgccgc acagtgccga
     6781 cgctggaaaa ctggcaactg gacttacagg gcatttccga caagctggac ggcgtaaaag
     6841 tggtctatgt ttgcagcccc aacaacccaa ccgggcaact gatcaatccg caggattttc
     6901 gcaccctgct ggagttaacg cgcggtaagg cgattgtggt tgccgatgaa gcctatatcg
     6961 agttttgccc gcaggcatcg ctggctggct ggctggcgga atatccgcac ctggctattt
     7021 tgcgcacact gtcgaaagct tttgctctgg cgggccttcg ttgcggattt acgctggcaa
     7081 acgaagaagt catcaacctg ctgatgaaag tgattgcccc ctacccgctc tcgacgccgg
     7141 ttgccgacat tgcggcccag gcgttaagcc cgcagggaat tatcgccatg cgcgaacggg
     7201 tggcgcaaat tattgcagaa cgcgaatacc tgattgccgc actgaaagag atcccctgcg
     7261 tggagcaggt ttttgactct gaaaccaact acattctggc gcgctttaaa gcctccagtg
     7321 cagtgtttaa atctttgtgg gatcagggca ttatcttacg tgatcagaat aaacaaccct
     7381 ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga agaaagccag cgcgtcattg
     7441 acgccttacg tgcggagcaa gtttgatgag tcagaagtat ctttttatcg atcgcgatgg
     7501 aaccctgatt agcgaaccgc cgagtgattt tcaggtggat cgttttgaca aactcgcctt
     7561 tgaaccgggc gtgatcccgc aactgctgaa gctgcaaaaa gcgggctaca aactggtgat
     7621 gatcactaat caggatggtc tgggaacaca aagtttcccg caggcggatt tcgatggccc
     7681 gcacaacctg atgatgcaga tattcacctc gcaaggcgta cagtttgatg aagtgctgat
     7741 ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaaa ccgaaagtaa aactggtgga
     7801 gcgttatctc gctgagcaag cgatggatcg cgccaacagt tatgtgattg gcgatcgcgc
     7861 gaccgatatt caactcgctg aaaacatggg cattaatggt ttacgctacg accgcgaaac
     7921 cctgaactgg ccaatgattg gcgagcaact caccagacgt gaccgttacg ctcacgtagt
     7981 gcgtaatacc aaagagacgc agattgacgt tcaggtgtgg ctggatcgtg aaggtggcag
     8041 caagattaat accggcgttg gcttctttga tcacatgctg gatcagatcg ccacccacgg
     8101 cggtttccgt atggaaatca acgtcaaagg cgacctctat atcgacgatc accacaccgt
     8161 cgaagatacc ggcctggcgc tgggcgaagc gttaaaaatt gccctcggcg ataaacgcgg
     8221 tatttgccgc tttggttttg tgctgccgat ggacgaatgc cttgcccgct gcgcgctgga
     8281 tatctctggt cgcccgcacc tggaatataa agccgagttt acctaccaac gcgtgggcga
     8341 tctcagcacc gagatgatcg agcacttctt ccgttcgctc tcttacacca tgggcgtgac
     8401 gctccacctg aaaaccaaag gtaaaaacga tcatcaccgt gtagagagcc tgttcaaagc
     8461 ctttggtcgg accctgcgcc aggccatccg cgtggaaggc gataccctgc cctcgtcgaa
     8521 aggagtgctg taatgaacgt ggtgatcctt gatactggct gcgccaacct gaactcggtg
     8581 aagtctgcca ttgcgcgtca cggttatgaa cccaaagtca gccgtgaccc ggacgtggtg
     8641 ttgctggccg ataaactatt tttacccggc gttggcactg cacaagcagc gatggatcag
     8701 gtgcgtgagc gcgagctgtt tgatctcatc aaagcctgta cccaaccggt gctgggcatc
     8761 tgcttaggga tgcaactgct ggggcggcgc agtgaagaga gcaacggcgt cgatttgctg
     8821 ggcatcatcg acgaagacgt gccgaaaatg accgactttg gtctgccgct gccgcatatg
     8881 ggctggaatc gcgtttaccc gcaggcaggc aaccgcctgt ttcaggggat tgaagacggc
     8941 gcgtactttt actttgttca cagctacgca atgccagtca atccgtggac catcgcccag
     9001 tgtaattacg gcgaaccatt caccgcggcg gtacaaaaag ataacttcta cggcgtgcag
     9061 ttccatccgg agcgttctgg tgccgctggc gctaagttgc tgaaaaactt cctggagatg
     9121 tgatgattat tccggcatta gatttaatcg acggcactgt ggtgcgtctc catcagggcg
     9181 attacggcaa acaacgcgat tacggtaacg acccgctgcc gcgcttacag gattacgccg
     9241 cacaaggtgc cgaagtgctg catttggtgg atctgaccgg ggcaaaagat ccggcaaaac
     9301 gtcaaatccc gctgattaaa accctggtcg ctggcgttaa cgttccggtg caggttggcg
     9361 gcggcgtgcg tagcgaagaa gacgtggcgg cgttactgga agctggggtt gcgcgcgtgg
     9421 tggttggctc cactgcggtg aaatcaccag aaatggtgaa aggctggttt gaacgtttcg
     9481 gtgccgatgc cttagtgctg gcgctggatg tccgtattga cgagcaaggc aacaagcagg
     9541 tggcggtcag cggctggcaa gagaattcgg gcgtgtcact ggaacaactg gtggaaacct
     9601 atctgcccgt cggcctgaaa catgtgctgt gtaccgatat ctcgcgcgac ggcacgctgg
     9661 caggctctaa cgtctcttta tatgaagaag tgtgcgccag atatccgcag gtggcatttc
     9721 agtcatccgg cggcattggc gacattaacg atgttgctgc cctgcgtggc actggcgtgc
     9781 gcggcgtaat agttggtcgt gcattactgg aaggtaaatt caccgtgaag gaggccatct
     9841 catgctggca aaacgcataa ttccatgtct cgacgttcgt gatggtcagg tggtaaaagg
     9901 cgtacagttt cgcaaccatg aaatcattgg cgatatcgtg ccgctggcaa aacgctacgc
     9961 cgaagaaggt gcagacgaac tggtgttcta cgatatcacc gcttccagcg atggccgtgt
    10021 ggtagataaa agttgggtat ctcgcgtggc ggaagtgatc gacattccgt tctgcgtggc
    10081 gggtgggatt aagtctctgg aagatgccgc gaaaattctt tcctttggcg cggataaaat
    10141 ttccatcaac tcccctgcgc tggcggaccc gacgttaatt actcgcctgg cggatcgatt
    10201 tggcgtgcag tgtattgtgg tgggtattga tacctggtac gacgctgaaa ccggtaaata
    10261 tcatgtgaat caatataccg gcgatgaaag ccgcacccgc gtcactcagt gggaaacgct
    10321 cgactgggta caggaagtgc aaaaacgcgg tgccggagaa atcgtcctca acatgatgaa
    10381 tcaggacggc gtgcgtaacg gttacgacct cgaacaactg aaaaaagtgc gtgaagtttg
    10441 ccacgtcccg ctgattgctt ccggtggcgc gggcaccatg gaacacttcc tcgaagcctt
    10501 ccgcgatgcc gacgttgacg gcgcgctggc agcttccgta ttccacaaac aaataatcaa
    10561 tattggtgaa ttaaaagcgt acctggcaac acagggcgtg gagatcagga tatgttaaca
    10621 gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg gactgatgcc ggtgattgtg
    10681 caacacgcgg tatccggcga agtgttaatg ctgggctata tgaacccgga agccttagac
    10741 aaaaccatcg aaagcggcaa agtcaccttc ttctcgcgca ctaaacagcg actgtggacc
    10801 aaaggcgaaa cgtcgggcaa tttcctcaac gtagtaaata ttgcgccgga ctgcgacaac
    10861 gacacgctac tggtactggc gaatcctatc ggaccaacct gccacaaagg caccagcagc
    10921 tgcttcggcg acaccgctca ccagtggctg ttcctgtatc aactggaaca attgctcgcc
    10981 gaacgcaaat ctgccgaccc ggaaacctcc tacaccgcca aactgtatgc cagcggcacc
    11041 aaacgcattg cgcagaaagt gggcgaagaa ggcgtggaaa ccgcactggc cgcgactgta
    11101 catgaccgct ttgagctgac caacgaggcg tctgacttga tgtatcacct gctggtattg
    11161 ttgcaggatc aggatctgga tttaacaacg gtgattgaga acctgcgtaa acgtcatcag
    11221 tgagttgcgg ggtaagcgga tgcgatattg ttgtcgcatc cggcaaaaaa aacgggcaag
    11281 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tgtaattacg
    11341 cagagcgtta cgccccagca caatccccgc gccaaccatg ccgcccagca gcaccgccag
    11401 aatcaaggtg attgcctttt tcggactatc gcgacgaata ggtaacgtcg gtttcattac
    11461 atagcggtaa gcatgaatat caagatcatc aacctttaag ttttcaatat ccaacaggtt
    11521 ttgacgtgtc tgatagtagt ttggtgagaa caccaacgga cgggtcgcct catgcttaat
    11581 catcgactcc agcgcttcgc tccccaaaag gaacaacgta tcttgcgtca catcttcagt
    11641 ctgctgaacc tgtggctttg ccacctgcgc ctgattcgca tactgcaacg cttcctgaat
    11701 ctgacggata cgcagctctt tctgctcctg cgcaactact tcctgcgttc ttaaagagtc
    11761 ctgcaagttt ttccgtccca gagcaatgtt gtctttgaga tccttttcta gctcttgatt
    11821 cactttatca tcaacttgct gaatgtattg ggccaacttc atttgtgctc cctctgcagt
    11881 ttgcccaaca taagaaacag tcaatggtaa ttgctggttc ttaacagaag gttcgatggt
    11941 aagtttttct ggttcttcct gattatccag cgtttctgct aatgctgaga aggcagaact
    12001 gaagcgacca atcaacgtct cctgcaaatc cgatactttc ggtgcagcct gaccatagat
    12061 aacattcatg gcattgttat agccagcaat ttgccccacg tcgggctgag tgataattgc
    12121 tgttgacgtc catttctcct tcgctactgc caaataacca atagccagag caatagccac
    12181 aatgacggaa atgatgattg tcatcttgcc acgccacaac tgcactagta aatcaatcaa
    12241 atcaatctgt tccgggtcat ggttttgccc agaaacatta ttattttcta ctctcatcat
    12301 taccctaact gacggaaatt tccaagtccg catagtttag cgataatccc tgtgaatgct
    12361 ataggataag tgataaaaat aatgaattat tgcggaggta acaaaagaca atcagggcgt
    12421 aaatagccct gataacaaga tgttagtcgc tgccaaagag atcgcgggtg tagaccttat
    12481 ctgccacatc cctaagctct tctgccatac ggttggaaat aatgacgtcg gcttgttgtt
    12541 tgaaggtggc gagatcacgt tccaggcgag agttgaagaa tgagtcttct ttcatcactg
    12601 gctcgtagat gattacttca acacctttcg ccttgatacg cttcataatc ccctgaatag
    12661 aagacgcacg gaagttatct gaaccgctct tcataatcag acgataaata cctaccactt
    12721 gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt acgcgtgcgg ttagcatcga
    12781 caattgccga gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat
    12841 ctttcggcaa acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac
    12901 gtgggtcgag acaaacgcct tcgattattt gacgggaatt cagacctaaa ctttctgcgt
    12961 agctatccag ttcgttaaag tacgccacgc gcatcgcgag gtaggtgttt gcaaaaagtt
    13021 taatcgcttc tgcttcagtg gagtcggtaa acagggttgg gatattttgc ttaatcgcgc
    13081 cttcctgtaa taacgcagca aaacgttctg cgcgttccga acgctcaccg attacaatac
    13141 gtgacggatg aaggttatcg taaagagctt taccttcgcg gagaaattcc ggagagaaaa
    13201 taatattttc agtacgatat ttcttatgca tcgcttcggt aaaaccaacg ggaaccgttg
    13261 atttgatgac cataaccgca taaggattta tctcaactac gtctttaatt actgattcta
    13321 cactggatgt attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga
    13381 cataatcagc atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat
    13441 ctgattgcaa aaactgctga atttccttat caacaatagg agatatccga tcattcagca
    13501 tagcaacgcg tgacggtaaa atatctaatg ccacaacctc atgattttga gcgattagaa
    13561 gcccgtttga caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc
    13621 agaattaact taactgtgaa tcatgatgtt tttaacatcc tgataagagc taaaag
//
LOCUS       DAZFTN010000121        13581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFTN010000121 DAZFTN010000000
VERSION     DAZFTN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            335..1048
                     /gene="aphA"
                     /locus_tag="ACHKV2_004033"
     CDS             335..1048
                     /gene="aphA"
                     /locus_tag="ACHKV2_004033"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6941825.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1159..1575
                     /locus_tag="ACHKV2_004034"
     CDS             1159..1575
                     /locus_tag="ACHKV2_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO6941826.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1579..1935
                     /locus_tag="ACHKV2_004035"
     CDS             1579..1935
                     /locus_tag="ACHKV2_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6941827.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1970..4792)
                     /gene="uvrA"
                     /locus_tag="ACHKV2_004036"
     CDS             complement(1970..4792)
                     /gene="uvrA"
                     /locus_tag="ACHKV2_004036"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO6941828.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5047..5583
                     /gene="ssb1"
                     /locus_tag="ACHKV2_004037"
     CDS             5047..5583
                     /gene="ssb1"
                     /locus_tag="ACHKV2_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO6941829.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5682..5963)
                     /locus_tag="ACHKV2_004038"
     CDS             complement(5682..5963)
                     /locus_tag="ACHKV2_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO6941830.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6392..7978
                     /gene="pdeC"
                     /locus_tag="ACHKV2_004039"
     CDS             6392..7978
                     /gene="pdeC"
                     /locus_tag="ACHKV2_004039"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO6941831.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7981..8304)
                     /gene="soxS"
                     /locus_tag="ACHKV2_004040"
     CDS             complement(7981..8304)
                     /gene="soxS"
                     /locus_tag="ACHKV2_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO6941832.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8390..8854
                     /gene="soxR"
                     /locus_tag="ACHKV2_004041"
     CDS             8390..8854
                     /gene="soxR"
                     /locus_tag="ACHKV2_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO6941833.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9400..10749
                     /gene="ghxP"
                     /locus_tag="ACHKV2_004042"
                     /gene_synonym="yjcD"
     CDS             9400..10749
                     /gene="ghxP"
                     /locus_tag="ACHKV2_004042"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO6941834.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10900..12549
                     /locus_tag="ACHKV2_004043"
     CDS             10900..12549
                     /locus_tag="ACHKV2_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6941835.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12703..>13581)
                     /gene="espX5"
                     /locus_tag="ACHKV2_004044"
     CDS             complement(12703..>13581)
                     /gene="espX5"
                     /locus_tag="ACHKV2_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO6941836.1"
                     /translation="IPQTVNGGYDFSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDC
                     NMYKTNFNFAIMEKILFDNCILDDSYFAQIKMTDGTLNSCSAMHVQFYNATMNRANIK
                     NTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSFSKLDLINFKHADLSRVNLNKAI
                     LQNINLIDSKLFFTRLTNTFLEMVICTDSNMANVNFNNANLNNCHFNCSVLTKAWMFN
                     TRLYRVNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNT
                     HEVAMKITADIMQHAD"
BASE COUNT         3218 a         3287 c         3510 g         3566 t
ORIGIN      
        1 aatcatcttt ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg
       61 tacagcagat aaatgttcta tcaaatttcg ctcatttgca gagtattcat cataataaac
      121 gtaaaattaa tgtatcctta catcgagcat taaccatttt ttacaaaaaa acgacaggaa
      181 cgtatttact aggttaaata taatcatcct gcttttcatc acaaaaaccg cagataatcc
      241 ttcctttccc ctgtagctgg cgttatggtc agatagtttt tgcaacaaat ctcacaataa
      301 aaagtttcaa catactgact atttagggaa aaatatgcgc aagatcacac aggcaatcag
      361 tgccgtttgc ttattgttcg ctctaaacag ttccgctgtt gccctggcct catctccttc
      421 accgcttaac cctgggacta acgttgccag gcttgctgaa caggcaccaa ttcattgggt
      481 ttcggtcgca caaattgaaa atagcctcgc agggcgtccg ccaatggcgg tggggtttga
      541 tatcgatgac acggtacttt tttccagtcc gggcttctgg cgcggcaaaa aaaacttctc
      601 gccagaaagc gaagattatc tgaaaaaccc tgtattctgg gaaaaaatga acaacggctg
      661 ggatgaattc agcattccaa aagaggtcgc tcgccagctg attgatatgc atgtacgccg
      721 tggtgacgcg atcttctttg tgactggtcg tagcccgacg aaaacagaaa cggtttcaaa
      781 aacgctggcg gataattttc atattcctgc caccagcatg aatccggtga tctttgcggg
      841 cgataaacca gggcaaaata caaaatcgca atggctgcag gataaaaata tccgaatttt
      901 ttatggcgat tccgataacg atattaccgc cgcacgcgat gtcggcgctc gtggtatccg
      961 cattctgcgt gcctccaact ctacctacaa acccttgcca caagcgggtg cgtttggtga
     1021 agaggtgatc gtcaattcag aatactgaca gagcgggaga gcgtgacact ctcccgaatg
     1081 ctgttttttt aatcacacct ttatcctttc gctgtcttgc tgcaaactga ttaagagagt
     1141 tttatcaagg agcagcacat gtggtatcaa aagacgctca cgcttagcgc caaatctcgt
     1201 gggtttcatc tggtaacgga tgaaattctg aatcagctgg ctgatatgcc gcgcgttaac
     1261 atcggcttac tgcatctgtt gctgcaacat acctccgcct ctctgacact taatgagaac
     1321 tgcgatccca ccgtacgcca cgacatggag cgttttttcc tccgcaccgt tcccgacaac
     1381 ggaaattatg agcatgacta tgagggagca gacgatatgc cttctcatat caaatcctca
     1441 atgctgggaa catcgcttgt attgccggtg cataaagggc gtattcagac cggcacctgg
     1501 caaggcattt ggctggggga acatcgcatc cacggcggat cgcgtcgcat catcgcgaca
     1561 ctacaagggg agtaaaaaat gaccatttcg gagttgctac aatattgcat ggcaaaacca
     1621 ggcgcagaac agagcgtgca taacgactgg aaagcgacgc agatcaaagt ggaagatgta
     1681 ctgtttgcga tggtgaaaga agtagaaaat cgcccagctg tttcgctgaa aaccagcccg
     1741 gagctggcgg agctgctacg tcagcagcac agcgatgtgc gtccaagccg ccatctgaat
     1801 aaagcgcact ggagcaccgt gtatctcgac ggttcgctgc cggattcgca aatctattat
     1861 ctggtggatg cgtcttatca gcaggcggtg aatttactgc cggaagaaaa acgtaaattg
     1921 ctggtgcaac tctgaaagga aaaggccgct cggaaggcgg ccttaacgat tacagcatcg
     1981 gcttgaggaa gcgtgccgta tgcgaagctt cgcactccgc gacggtttct ggcgtaccgg
     2041 agacgaggat ctcgccgccg ccactgccgc cttccggccc caggtcgaca atccagtcag
     2101 cggttttaat cacgtcgaga ttgtgctcaa tcaccacaat ggtgttgccc tgatcgcgca
     2161 gtttatgcag tacgtcgagc agttgctgaa tatcggcgaa gtgcagaccg gtggtcggct
     2221 cgtcgagaat atacagcgtc tgcccggtgc cgcgttttga cagctcacgc gccagcttca
     2281 cgcgctgggc ttcaccacca gaaagtgtgg ttgcggactg ccccaagcga atgtacgtca
     2341 gaccaacgtc catcaacgtt tgcagcttac gcgccagagc tggcaccgca tcaaagaact
     2401 cacgcgcctc ttcgatggtc atatccagca cttcgtggat ggttttgcct ttgtacttaa
     2461 tctccagcgt ttcacggtta tagcgtttac ctttgcactg gtcgcacggc acgtaaatgt
     2521 ccggcaggaa gtgcatctcc actttgatca cgccatcgcc ctgacaggcc tcgcagcgtc
     2581 cgccacggac gttaaagctg aaacgtcctg gcgtataacc acgcgcacgg gattccggta
     2641 cgcccgcaaa cagttcgcgc acaggcgtaa acacgccggt ataggtcgcc gggttagaac
     2701 gcggagtacg accaattggg ctttggtcga tatcgattac tttgtcgaaa tgctccagtc
     2761 cctgaatatc gcgatacggt gccggttcgg cgatggtcgc accattcaac tggcgttggg
     2821 caatcgggaa cagtgtgtcg ttaatcagcg tcgatttacc ggaacctgaa acccctgtga
     2881 tgcaggtaaa cagaccgact ggcagcgtta gcgtcacgtc tttcaggttg ttgccgcgtg
     2941 cgccagtcag cttaagtact ttttccggat tcgccggaac gcgtttcttc ggcacttcaa
     3001 tcttgcgttt accgctcatg tactgtccgg tcaacgactc cggcaccgcc ataatcgctt
     3061 ccagcggacc ttccgcgacc acttcaccgc catgtacacc tgcaccagga ccgatatcga
     3121 tcacatgatc agcggcgcga atcgcgtctt cgtcgtgctc caccacaatc acggtattac
     3181 cgagatcgcg cagatggata agcgtaccca acaggcgctc gttatcgcgc tggtgcaggc
     3241 cgatagacgg ctcatccagc acgtacataa cgccaaccag gcccgcacca atctggctcg
     3301 ccagacggat acgctgggct tcaccgccgg aaagcgtttc tgccgagcgg gaaagcgtca
     3361 ggtaattcag gccgacgtta acgaggaatt tcaaacgatc gccgatctct ttaagaattt
     3421 tttccgcaat cttcgcccgc tgacctgcga gtttgagatt gttgaagaat tccatcgcat
     3481 gaccaatgct catgtcggag atggcgggca gcggcgtatt ctcgacatac acgtggcgcg
     3541 cttcccgacg cagacgcgtc ccttcgcagc tggcgcacgg gcgattgctg atgaacttgg
     3601 ctaattcttc acgtaccgca ctggattctg tctctttata acggcgctcc atattgtgca
     3661 gcacgccttc gaacggatga cgacggatgg aggtatcgcc acgatcgttc atgtatttga
     3721 attcaatgtt ttctttgcca gaaccgtaca acaccacttt atgcacgttc gcgctcaggc
     3781 tgccccacgg cgcttcgacg tcgaacttat agtgatctgc cagcgatttc agcatctgga
     3841 aataatagaa gttgcggcga tcccagccac ggatcgcacc acctgccagc gacagttccg
     3901 gattctggat cacgcggtca ggatcgaaat attgctgtac gccaaggccg tcgcaggtcg
     3961 ggcaggcacc tgccgggttg ttaaacgaaa acagtcgcgg ctccagttca cgcatactgt
     4021 agccgcaaat tgggcaggcg aagttggcgg aaaacagcag ctcttccgct ttcgggtcgt
     4081 ccatatccgc cactaccgcg gtaccaccgg aaagctccag cgcggtttca aacgactcgg
     4141 caagacgttg ggtaagatcg tcacgcacct tgaagcgatc aaccaccact tcaatagtgt
     4201 gtttcttttg cagttccagt ttcggcggat cggaaagatc gcagacttcg ccatcaatac
     4261 gagcacggat gtaaccctgg cttgccaggt tctccagcgt tttggtgtgt tcgcctttgc
     4321 gctctttaat gattggcgcg agcagcatca gacgcttgcc ttccggctgc gacagcacgt
     4381 tatccaccat ctggctgacg gtttgcgccg ccagcgggac gtcgtggtcc ggacagcgcg
     4441 gttcgccgac gcgggcgtac aacaaacgca aatagtcgtg gatttcggtg attgtcccca
     4501 ccgtagaacg cgggttatga gacgtcgatt tctgctcaat tgagatggca ggagaaagcc
     4561 cctcaatatg atcgacgtcc ggcttttcca tcagtgacag aaactgccgc gcgtaggcgg
     4621 aaagggattc aacgtaacgg cgctgccctt cggcatataa ggtgtcgaaa gcgagcgagg
     4681 atttgccaga acccgaaagc ccggtcacga caatgagctt gtcgcggggg ataacgaggt
     4741 tgatgttttt gagattatgg gtgcgggcgc cccgaacttc gatcttatcc attcaccttt
     4801 cccggattaa acgctttttt gcccggtggc atggtgctac cggcgatcac aaacggttaa
     4861 ttatgacaca aattgacctg aatgaatata cagtattgga atgcattacc cggagtgttg
     4921 tgtaacaatg tctggccagg tttgtttccc ggaaccgagg tcacaacata gtaaaagcgc
     4981 tattggtaat ggtacaatcg cgcgtttaca cttattcaga acgatttttt ttcaggagac
     5041 acgaacatgg ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac
     5101 ccggaagtac gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc
     5161 gaatcctggc gtgataaagc gaccggcgag atgaaagagc agactgaatg gcaccgcgtt
     5221 gtgctgttcg gcaaactggc agaagtggcc agcgaatatc tgcgtaaagg ttctcaggtt
     5281 tatatcgaag gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac
     5341 accacagaag tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt
     5401 ggtggcgctc ctgcaggtgg caatatcggt ggtggtcagc cgcagggcgg ttggggtcag
     5461 cctcagcagc cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag
     5521 tccgctccgg cagcgccgtc taacgagccg ccgatggact ttgatgacga tattccgttc
     5581 tgatttgtca ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc
     5641 agccataaaa aagaagcctc cgttatggag gcttctacgt atcaggtcaa aatcattggc
     5701 cactgtggcg gtgtctgatt catctcttct atcatcaccg actcgatgtt gtgccagatg
     5761 gcggtgatgt ccgccattgt gatgccaagc aaccccattg cgaaccagca ggcggaaacc
     5821 acgcccagac cgctgctgat cactgcaagc ccgacataat cagaccgacg aaaacggatg
     5881 ttgagcgtgc tggccagaaa catcattacg gcactaagaa gttgccagcg aagaagaacc
     5941 acgccagtgg tgagggtagc catcaaacaa ttcctctgaa aagagccgat gccctggaca
     6001 gcgcggcgtt gttcacggga ggcgtggtac actctggcta tcgcggggct tgcagaacac
     6061 aaaaatgaaa cactctgttt gtttcattaa ttttgtgaac tatatcacaa ttgattgttt
     6121 gttagccaga ttaggtcgtg acttttattg ctgtacagat tatgtggttt ttcagtggca
     6181 ttaagggtat atcttcccgc cgcctctgta ttcctgtaga aaattaattt tgaatatcaa
     6241 tgaattattt tcatccaagt gacgattaaa aaggtatcaa tttcaaatca ggcaaaagtg
     6301 ctatttatac cgtaagattt atctaaagac gtcggtagcc agggtttcac cttgcaatgg
     6361 ccgggtataa acaggcagga aattgatagc aatgaatcat cgtgcacggc accaattact
     6421 ggcgttgccg ggcattatct ttttagttct ctttcccatc attctttcgc tatggattgc
     6481 cttcctttgg gcaaaatcag aagtgaataa tcagctccga acctttgctc aactggcgtt
     6541 ggataaatcc gagctggtca ttcgccaggc agatttagtg agcgatgcag ctgaacgcta
     6601 tcaggggcaa gtttgcactc cagcccatca aaagcgaatg ttgaatatta ttcgtggcta
     6661 tctttatatt aatgaattga tctatgcccg tgataatcat tttttatgct catcgctgat
     6721 agtgcctgta aacggctata cgattgtatc ggccgattat aagcgtgaac ctaatgtttc
     6781 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
     6841 gggaaattat gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
     6901 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
     6961 ggcctcagca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg taccaagaaa
     7021 tggctattta tatgcgacag tttattcgac aaaacgccca attgcagcca ttgttgcgac
     7081 ttcatatcaa cgtcttatag cccattttta taatcatctt atttttgcgt tgcccgccgg
     7141 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttgtc
     7201 tcccaaacgc aaattgcaac gcgccctcga aaaacatcag ctatgccttt attaccagcc
     7261 aataattgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
     7321 tgagcagggg caagtaatga atccggcaga gtttattcct ctggcagaaa aggaggggat
     7381 gatagaacag gtaactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
     7441 tgcaacacat gctgatcgct atgtttctat taatctgtcg gcctccgatt ttcatacgtc
     7501 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
     7561 taaatttgaa gtgactgagc atgcatttct tgatgtcgac aaaatgacgc cgattattct
     7621 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
     7681 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgacaaat cgtttgttga
     7741 gacgctgacc acccataaaa ccagtcattt gattgctgaa catatcatcg agctggcgca
     7801 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     7861 gcgcaaacgc ggcgtgcgct attgccaggg atggttcttt gcgaaggcga tgccgccgca
     7921 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     7981 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     8041 gaaggtctgc tgagagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     8101 ggtgcgcaac tcaacggcgg ccagtaacag acggcgttga cgaatgtaat cgccaagcgt
     8161 ctgatgcgtc accgtgcgga acatccgttg caggtaccac tttgaatagc ctgatttttt
     8221 tgcgacgaca tcaatgttaa gcggctggtc aatatgctcg tcaatccatg cgataagatc
     8281 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     8341 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     8401 attaccccgc attaaagcgc tgctaacccc cggcgaagtg gcgaaacgca gcggtgtggc
     8461 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt accagtatcc gtaacagcgg
     8521 caatcagcgg cgttataaac gtgatgtgtt gcgatatgtc gcaattatca aaattgctca
     8581 gcgtattggc attcctctcg cgaccattgg tgaagcgttt ggcgtgttgc ccgaagggca
     8641 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     8701 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     8761 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     8821 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaagggcgc tttagtttgt
     8881 tttccggtct ttgtctttct ctctatcccg ctggtacaca ggagggtttc ccccgacgtc
     8941 aacacacctc attcgagcac gtggtggagg ttccggttgg tgttgatgct ttaattgtat
     9001 gtcaccgacg tttcttcgcc agtgtaaaag tatacttttt agccgcaata tttttgtcat
     9061 ctcagacgat tttttatcgc aatcctcaac ggtatacggc tcgataacgc tgcaatcttg
     9121 cgcatcgacg ataacgtttg cgcatcaatt gcctggtttt tcatcgtcaa gacaataaaa
     9181 gagaaaaaag cagcaaactt cggttgaaaa agccgctatg atcgccggat aatcgtttgc
     9241 tttttttacc acccgttttg tatgcgcgga gctaaacgtt tgcttttttg cgacgcagca
     9301 aattgtcgca aacctggagc aggaagataa cgtttcgctg gcaggggatt gtccgccacg
     9361 catcttgacg aaaattaaac tctcagggga tgttctctta tgtctacgcc atcagcgcgt
     9421 accggcggtt cactcgacgc ctggtttaaa atttcacaac gtggaagcac tgtccgtcag
     9481 gaagtggttg ccgggttaac aacgtttctg gcgatggtct actcggttat cgtcgttcca
     9541 ggcatgttgg gtaaagcggg cttcccgcct gcggcagttt tcgttgcgac ctgtctggtt
     9601 gccggactcg gttctatcgt gatgggtctg tgggctaatc tgccgttggc gattggttgc
     9661 gccatctccc tgacggcgtt taccgcattc agcctggtgc tggggcaaca tattagcgta
     9721 cctgtcgcgc tgggtgccgt gttcctgatg ggtgtgctgt ttacggtaat ttctgccacg
     9781 ggtatccgta gctggatttt gcgcaacttg ccgcacggtg tggcgcacgg cacggggatc
     9841 ggtatcggtc tgttcctgct gctcattgcc gctaacggtg tcggtctggt gattaaaaac
     9901 ccgcttgatg gtctgcccgt tgcgctgggt gatttcgcga ccttcccggt gattatgtca
     9961 ctggtaggtc tggcggtgat catcggcctg gaaaaactga aagtccctgg tggcattctg
    10021 ctgaccatta tcggtatctc aattgtcggt ttgatcttcg atcctaacgt ccatttctcc
    10081 ggcgttttcg ccatgccttc attgagcgat gaaaacggca attcactgat tggcagcctg
    10141 gacattatgg gcgcgctgaa tcctgtagtc ctgccaagcg ttctggcgct ggtgatgacg
    10201 gcagtatttg atgccaccgg aactatccgc gctgtcgccg gccaggcgaa cctgctggat
    10261 aaagatgggc agatcatcga cggcgggaaa gcactgacca ctgactccat gagcagcgtt
    10321 ttctctggcc tggtgggtgc agctccggca gcggtataca tcgagtctgc ggcgggtacg
    10381 gcggcgggcg gtaaaaccgg tttgacggct atcaccgttg gcgtgctgtt cctgctgatt
    10441 ctgttcctct ctccgctttc ttacctcgtt ccggggtatg caacggctcc ggcgctgatg
    10501 tacgttggcc tgctgatgct gagcaacgtg gcgaaaatcg actttgctga ttttgttgat
    10561 gcgatggcgg gtctggttac ggcggtattc atcgtgctga cctgtaacat cgtaacaggc
    10621 atcatgatcg gcttcgcgac tctggtgatt ggtcgtctgg tttccggtga atggcgcaag
    10681 ttgaacatcg gtacggtcgt tatcgccgtg gcgctggtga ccttctatgc gggtggctgg
    10741 gctatctaat ctttcatcgg attctgaaaa cgggtggcaa tggctgcccg tttttatttt
    10801 ctccgcacat tgtgtgagct tttgcgatat tctgaaaaaa tgagaattca ggcataacgt
    10861 ctttctcaga tcataaaaaa catcgcaaac agggaacgca tggaaatctt cttcaccata
    10921 ctgataatga ccctcgtggt ctcgctgtcc ggggtggtca ctcgtgtcat gccctttcag
    10981 atcccgcttc cgcttatgca aatcgccatc ggtgcgctac tggcgtggcc gacgtttggt
    11041 ttgcatgtgg agtttgatcc tgaactcttt ttagtcttgt ttatcccgcc gttgctgttc
    11101 gctgatggct ggaaaacgcc gacccgtgaa tttctcgaac atggtcgaga gattttcggc
    11161 ctcgcgctgg cgctggtggt ggtcaccgtg gtcggcattg gcttccttat ttactgggtg
    11221 gtgccgggca ttccgctgat ccccgccttt gcgctggcgg cggtgctttc tccgaccgat
    11281 gctgtggcgc tctccgggat tgttggcgaa gggcgcatcc cgaaaaaaat catgggcatt
    11341 ttgcagggcg aagcgttgat gaatgacgcc tccggcctgg tgtcgttgaa gtttgccgtg
    11401 gcagtggcga tggggacgat gatcttcacc gtcggcggtg cgacggtcga atttatgaaa
    11461 gtagccattg gcggtattct cgccggtttt gtggtgagct ggctgtacgg tcgctcgctg
    11521 cgattcctca gccgctgggg cggtgatgaa cccgcgacgc agatcgtcct gctgttcctg
    11581 ctgccattcg cttcttatct gattgccgaa catattggcg tttccggcat cctcgctgcg
    11641 gttgccgccg ggatgaccat cacccgctcc ggtgtgatgc gccgtgcgcc gctggcaatg
    11701 cgcctgcgtg caaacagcac ctgggcgatg ctggaatttg tctttaacgg catggtgttc
    11761 ctgctgttag gtctgcagct gccgggtatt ctggagacgt cgctgatggc agcagaaatc
    11821 gaccctaacg tcgaaatctg gatgctgttt accgatatta ttctgatata tgcggcgctg
    11881 atgctggtcc gtttcggctg gctgtggacg atgaaaaagt tcagcaaccg cttcctgaag
    11941 aagaagccga tggagtttgg ttcgtggacc acacgagaaa tcctgatcgc gtctttcgcc
    12001 ggggtgcgtg gggcgatcac tttggccggt gtgctctcta tcccgctgct cttgccggat
    12061 ggtaacgtct tcccggcgcg ctatgagctg gtgtttctgg cggctggtgt cattctcttc
    12121 tcgctgtttg tcggcgtggt gatgttgcct attctgctac aacacattga agtcgcggac
    12181 cattcgcaac aattgaaaga agaacgtatt gcgcgagcgg caacggcaga agtggcgatt
    12241 gtggcgatcc agaaaatgga ggagcgtctg gcggcggata ccgaagagaa tatcgataac
    12301 cagctgctta cggaggtcag ttctcgcgtc attggtaacc tgcgtcgtcg cgccgatgga
    12361 cgtaacgacg ttgaaagttc cgtgcaggaa gagaaccttg agcgtcgctt ccgtctggcg
    12421 gcattgcgtt ctgaacgtgc tgaactgtac cacctgcgcg ccacgcggga gatcagcaac
    12481 gaaacgctgc aaaaattact gcacgatctc gatttgcttg aagcgttgct aattgaggaa
    12541 aatcagtaag ccgaaagcga cgtaaccagg cccgaggtaa tctgaaaaag gtgcaatctt
    12601 ttgcaccttt aacaggttag ctcgggctaa cattcatgat tctaaaacaa ataagttatc
    12661 tctcttttaa agtcatttca ttggctgttc ttcgacttaa tctcaatctg cgtgttgcat
    12721 aatatctgca gtaatcttca tagctacttc atgagtatta ttctcagttt gtgacatgcc
    12781 agtatgagag gtacaatctt cttcaaagaa tttctgtagt gttaccaggg tatcactatt
    12841 aatgggaata ttctcctccc cacggagaat ggaaatgccc attccctgga cgctagcctc
    12901 atcgaaatta acccgataga gacgggtatt aaacatccag gcttttgtta aaacagaaca
    12961 gttgaaatgg caattgttta aattggcatt attaaaatta acattagcca tattagaatc
    13021 ggtgcatata accatttcga ggaacgtatt tgttagccgt gtaaaaaaga gtttactatc
    13081 aattaagttt atattctgga ggattgcttt gttcagattg acgcgagaca gatcagcatg
    13141 tttaaagtta attaaatcaa gtttagagaa gctaaggtcg gctctaaata aattaatata
    13201 tggcgctatt actttataaa gatttacctc agccatgtat gccatataaa aatttgaata
    13261 atcaaggaag gtatttttaa tattggctct attcattgtt gcattgtaga attgaacatg
    13321 catagcggag catgaattta gagttccgtc agtcattttt atctgagcga aatatgagtc
    13381 atcgagaata caattatcaa aaagtatttt ttccattatg gcgaaattaa aattcgtttt
    13441 atacatatta cagtcctgga aaatagcgtt ttgtagtctg caacctgcaa aattcgaatt
    13501 ggataaatct tcatctttaa tcacgatacc tttcaggcta aggtgtgaaa aatcgtaacc
    13561 accattcact gtctgtggga t
//
LOCUS       DAZFTN010000122        13501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFTN010000122 DAZFTN010000000
VERSION     DAZFTN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..935
                     /locus_tag="ACHKV2_004045"
     CDS             105..935
                     /locus_tag="ACHKV2_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6941837.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1076..1849)
                     /gene="uxuR"
                     /locus_tag="ACHKV2_004046"
     CDS             complement(1076..1849)
                     /gene="uxuR"
                     /locus_tag="ACHKV2_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO6941838.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2064..3524)
                     /locus_tag="ACHKV2_004047"
     CDS             complement(2064..3524)
                     /locus_tag="ACHKV2_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO6941839.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3605..4789)
                     /gene="uxuA"
                     /locus_tag="ACHKV2_004048"
     CDS             complement(3605..4789)
                     /gene="uxuA"
                     /locus_tag="ACHKV2_004048"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO6941840.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5129..6472
                     /gene="gntP"
                     /locus_tag="ACHKV2_004049"
     CDS             5129..6472
                     /gene="gntP"
                     /locus_tag="ACHKV2_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO6941841.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6646..7548)
                     /gene="fimH"
                     /locus_tag="ACHKV2_004050"
     CDS             complement(6646..7548)
                     /gene="fimH"
                     /locus_tag="ACHKV2_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6941842.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTKNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7568..8071)
                     /gene="fimG"
                     /locus_tag="ACHKV2_004051"
     CDS             complement(7568..8071)
                     /gene="fimG"
                     /locus_tag="ACHKV2_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO6941843.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8084..8614)
                     /gene="fimF"
                     /locus_tag="ACHKV2_004052"
     CDS             complement(8084..8614)
                     /gene="fimF"
                     /locus_tag="ACHKV2_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO6941844.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8624..11260)
                     /gene="fimD"
                     /locus_tag="ACHKV2_004053"
     CDS             complement(8624..11260)
                     /gene="fimD"
                     /locus_tag="ACHKV2_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO6941845.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11327..12052)
                     /gene="fimC"
                     /locus_tag="ACHKV2_004054"
     CDS             complement(11327..12052)
                     /gene="fimC"
                     /locus_tag="ACHKV2_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6941846.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12089..12628)
                     /locus_tag="ACHKV2_004055"
     CDS             complement(12089..12628)
                     /locus_tag="ACHKV2_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6941847.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12693..13241)
                     /gene="fimA"
                     /locus_tag="ACHKV2_004056"
     CDS             complement(12693..13241)
                     /gene="fimA"
                     /locus_tag="ACHKV2_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6941848.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3281 a         3471 c         3257 g         3492 t
ORIGIN      
        1 ctacaacaga aacatgcatg tcatttgttt acttctcttt ttactacaga aaaatttatc
       61 tatttttcta aacatcagtg aaatactcat aaaaggtaac tattatgtct atgccattaa
      121 gcaatgtcct tcagagtcaa attattacag ataatcactt ccttcatcat ccaaaagtcg
      181 aaagtgagtt aacacgtaaa tatgaacgtg cacgcctgga tacggaaaat atctatcttc
      241 tgcctcttgc tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      301 aacttgatat tagtaaagaa tcctggccat ttaattatgt gacagaagca tgcagagaat
      361 ccgatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
      421 tagacgaaat atatggtggt atatgtaaaa aggcgcatgc tgctacagag ttagcagaag
      481 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
      541 atgaaataac attaggccct ggttgcaatg ttccaggtta cgccggaacc acgataggat
      601 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaagaattg
      661 atatctatat tgatcagata ataactgtct caggggtagc aaattcttcc ggattcgccc
      721 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      781 cctatccagg taccgcggag atccatgcaa aaatgggata taaagttatc cccggtgatg
      841 aagatgcgcc actcaaacgc atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      901 taaaaaatgg tgaatggaat tatattggca aataaccact gagaagaatg agtactaaca
      961 ttcaggttat atagatattt aaacaatacc ctgcaattta caaagtaata ctcattgcga
     1021 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
     1081 cgtcgacttt atccagcggc catgaatcaa acagatagcc gtcaaaataa atatcgtcaa
     1141 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     1201 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1261 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attgggttgt
     1321 tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta tgcgttgctt
     1381 cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc gcactggaag
     1441 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1501 cctgcagggc ggcaaactcg gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
     1561 caaaaggacc cgcatcattg cagacgttgg catccggact gtctgtgttg tggctgcctg
     1621 agctatcaag aacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
     1681 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     1741 gctccggcgg tagccgttca ccaggattgt acggcgtctt tatgatcaga tcgcggatca
     1801 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
     1861 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1921 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
     1981 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     2041 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     2101 cacagctgct gatatgccgc agtcaccgcg ccaacaaagt cggcattctg cggcagatca
     2161 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2221 gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc attaccctgc
     2281 tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgccag gtgacgccag
     2341 ctgccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg taacttctgg
     2401 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2461 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     2521 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt catggtgtcg
     2581 gcaatagttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     2641 agcatacgca gtttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     2701 ttatcccagt ccggacgacc attaacgaag ttatcttcaa taacccactg acggaacggt
     2761 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     2821 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     2881 tcaatccatg ccgctagctg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     2941 gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     3001 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     3061 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     3121 tcagcgcagt agcctttttc cgtgaccgtc agggagacaa tcgccgtttg cggacgcgcc
     3181 atcgcgttga gaataccttc gcagccgtcg atttccgggt gcagcgcttc tttcatcgaa
     3241 ccgataattt tcagctcggt gctttccgcg cctttttccg ccacggtgta cagcagttgc
     3301 tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag
     3361 ataccccagt cgctgtcggt gctttccagc agatgatggg tgtacagcgc ctggtgcgca
     3421 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3481 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3541 cgtcttcaga gagtgacggc aaggaagaag gagccggcac ggcgcgcagc catgccggtg
     3601 ggtattaatg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     3661 ggcctttcag acgaccaatt gcggagtaac cagggttggt tttcttcttc aggtcgtcca
     3721 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3781 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     3841 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3901 tgaagtaaat acgcggaccg aactgcttga tcatatcaac caaatcgttg tcagcacgca
     3961 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     4021 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     4081 gatcgtccgg gtgaacagcc atacggacgc caacttcttc ggcaaccgga ataatcgctt
     4141 tcaggaagac agcaaagttt tcgcgcagtt tggctttgtc gatatctttg tacagctcca
     4201 ggtgcttacg gaactggtcg agggtgtacc cttcttccgc acctggcaga ccagcgatga
     4261 tgttacgggt cagacgcgct ttgtcttcat cgctcatagt ggcgaagcgt tcagctgcct
     4321 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     4381 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4441 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4501 tgccgcactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     4561 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     4621 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     4681 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4741 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4801 tttcacatag tgggttccac agcaaagaga cgcggaaccc tggagttgtt taggtatccg
     4861 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4921 cttttggtaa gacatgtcaa cccgaattct gaaattggtt aaccacatca caagaatttc
     4981 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     5041 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     5101 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     5161 cggcattggt ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5221 gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     5281 caccatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     5341 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cgcatacgct
     5401 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gcctgatttt
     5461 tggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgtcat
     5521 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     5581 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     5641 cgcagatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     5701 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5761 gaaagcagat caaccggtag atatgaacaa tctgccctct ttcggcgttt cgattctggt
     5821 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     5881 agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg cgatgtttat
     5941 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     6001 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     6061 gggtggcgtg ctgaagcaaa ctatcatcga caccggcatt ggcgacacca tcggcatgtt
     6121 gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg tgctaattcg
     6181 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
     6241 aatccttgat ccagcaactg gtcagctggt tggcgtgaat ccggcgctgc tggtgctggc
     6301 gacggctgcg ggttccaaca ccctcaccca cattaacgat gcatctttct ggttgttcaa
     6361 aggttacttt gacctgtcgg taaaagacac gttgaaaacc tggggtctgc tggagctggt
     6421 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaagaacac
     6481 cagagcctgc caatggcagg ctcagttcgg attgtcggca aagtgcgctt tgtttatgcc
     6541 agatgcgacg ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag gtttttagct
     6601 tcaggtaata ttgcgtacct gcattagcaa tgccctgtga tttctttatt gataaacaaa
     6661 agtcacgcca ataatcgatt gcacattccc tgcagtcacc tgcccgccgg tacgtgcgta
     6721 atttgccgtt aatcccagac ttaccgccga agttcctact gctcctaacg ataccgtgtt
     6781 attcgctgga ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga
     6841 aaacgacgcg gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta
     6901 ataccccagg ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg
     6961 gtagtccggc agagtaacgg tgacatcacg agcagaaaca tcgcagccgc cagtaggcac
     7021 taccacatca ttattggcgt aaatattcca cacaaactgg aaatcatcgc tgttatagtt
     7081 tttggtctgt cgcaaaataa gcacggcaat taatgagcca gctttaatcg ccaccccgcc
     7141 cgcactgctc acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt
     7201 ataaacaacc cgcggcgttt cgctggtggt cggaaatgga tagctactgc cactatattt
     7261 tacggtcccg gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac
     7321 atagtctgta atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac
     7381 gaccaggttt tgccccacat tcacggcagg cgcaaggttt acataaacat tagcgctgcc
     7441 accgccaata gggatagcgg taccattggc ggttttacag gcgaatgacc aggcatttac
     7501 cgaccagccc atcagcagta cagcaaacag ggtaataact cgtttcatta caattatctc
     7561 ttcgggttca gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg
     7621 ctccgccatt taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat
     7681 catccacctg aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta
     7741 gctctaactg gatgttttgc gcggtcccct ggtttttata atatccggta ctgtcggctg
     7801 ccccgctgaa gctggcagtg acccttgacg ttcccaccgg acaattagtc aactcaagcg
     7861 caacatcatg ccaggccgat gccgccccgg cagacatcag actgaaagaa taaagatcgc
     7921 cgagatcaac cgtggcattg gtggtggaaa ctgtgcacgg tttggcgacg accttaccgt
     7981 tcaccgtgat ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg
     8041 ccaataaata cccacgtttg cgccatttca tgagcatctc cagttactga tattcaagag
     8101 tgaaggtagc cgtggcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc
     8161 gggcgtaaaa attcaacgta tttggtttac ccggcgtcag ggtcgtccac gaaatcgcgg
     8221 acgatggagc attaaggggt atctgatttt gctgctcatt cagaagctgt attcccagtc
     8281 ccgaagccgc tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa
     8341 cgccggtaaa cccaactttt acggcagaaa cggcgttacc acagggtgac agcaaaatac
     8401 gaaatggaac gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca
     8461 tcagatcaac agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat
     8521 agccgcggat agtaatcgtg ctatccgcag ccaaagcgtg actcactgcc agccacaaaa
     8581 aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg
     8641 atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg gcgacacaat
     8701 gagcattttc ctcttctccc catttcacct gaacttttcc cgctagaggc attccgctga
     8761 ggtaaacctg accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca
     8821 tcgccccaaa cggcagcggc ttattattgt gggttagcgt catgagcagt tttatcccaa
     8881 cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg
     8941 cgttatctaa atcgacgtta tcagccaggg tattggtatc cagcgccact ctattttccc
     9001 gatattcagt ggcataaggc agcacggcat aaccgcgcca gtcggtacgc acccccgtct
     9061 ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta
     9121 acggctgccc cagcgttacg ccattggcat gagccagtac cccaccgctg actccgtaat
     9181 agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc
     9241 gataattcag cgtggcgtag cctgtgcttc cgctattacc atcgcctccc ccggcatagc
     9301 cggtttgcac gctatagctg aggttgttgt cttccagcaa cgtaccgtat acaccagcca
     9361 gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc
     9421 actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttacgcgcta
     9481 acatctgatc acgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt
     9541 tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc
     9601 cccaataagt ttgatggcta ccactcaaat acagtgttga tgagcgcccg agttgctgag
     9661 taacggtgag ttgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt
     9721 tcggcttaac ctgaataact ccgtcctgtg tttcgatgtt gtagccattc attcgactgt
     9781 atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa
     9841 tattcgtgcc tgactcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat
     9901 gctgactgtc atcgggaagt gtggaattag cctgagtcat atccacagac agagcgccca
     9961 gtgcccccat atttttcccg ataccaaaat taaaagcacg ataacgatct gccagttgcg
    10021 ttccaccata tattgtccag ccagctggaa ggccgtggag taatgtactt tggaaaaagc
    10081 ggggtttttc ctgttgcgca tttccactac ggtattctcc tgccgtaatg gaataacgag
    10141 tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc
    10201 tgccgtcagc ctctttaatc gttacctgca agtcaccact attacctgcg gcatagatat
    10261 cgttgatggt aaaaggcccc ggcggcaccg tactattata aatgtcatac ccattttgtt
    10321 taatagtgac ctgtgcagta ccacgagcaa taccgtggat caccggggca aatcctcttt
    10381 ggctatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat
    10441 cgaaaatatc accctgagta taaccatcac ccagcgtcag ccgggaacgt aacggtatta
    10501 tgtctcgctc aagccaggta ttgatatgct gccatttatt tttgctacct gatgatctgt
    10561 cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc
    10621 cactctgtag gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat
    10681 ttccgctgaa attataattg agcaatccgg cattaatacc gggatcccat aactcaggag
    10741 gaatataacc acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct
    10801 gctgaccaac atctaaatgc gcagtagcgt cctggaccat tgtggttaat ggcacacagg
    10861 catcatccgc cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga
    10921 gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga
    10981 catcacgcgt tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg
    11041 gtaattcttg cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta
    11101 aaaagcgcgg attaaaatag agttcggcag atgacaaagg tgcctgtacg gcaaaagcac
    11161 aggcgacaaa gagccggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt
    11221 gtgtgtttcg ctggtaaagt cttaaattca gatatgacat tattcctccg gtttaaaatc
    11281 acccggcagt caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc
    11341 ccgtcatttt gggggtaagt gcaccataat catttattgt tcggtaagta atattgcttc
    11401 ccgcatcaga aggcaattta accgtgcttt cgcccattgg aggcaccaag gcattttcaa
    11461 gaacccgggc tccggcattc aactctgtta ccgtcaggta atagggtgtc gggttaatca
    11521 gcgtcagaga attcgcgcta cgacgaaatc ttaatttttc tgcggcctga tcgggtggca
    11581 acgctaattt agccggacga tagtacagtt taatgcggct gataattgcg agctgtagcg
    11641 tattctcagt caattttgat ttatccattg acggaatcgc tttaacgttc atccagaata
    11701 aactttcccg gtcctgtggc aattggttat ttgttgcatc aagaatacgt aaggtattct
    11761 ctttttttcc cttcatcgca aacagaggag gcgtcacgat aaaacgacca tcctttacac
    11821 catcggcatt ttccacccat gattgaatta aataggtact attttcatca ttatttgtca
    11881 cggcaagttg cacttgtttt tgccctgccg gataaattat gcgagtcgca cctaaggcca
    11941 ctcccgcttc agcgcgtccg gcaaccatca ttgccatgaa catcaggata cctgccagca
    12001 agcagaatgt tatttcctgc gatttcctta cattgacgtt tttattactc actgtcctgt
    12061 tcctgttatc acattatctg ctgaacaatt actgataggt taaagagaac caggcctgtg
    12121 cattggcgat gccgccagta acccgacgcc cggtagcacg atatttggcg atgaaatgta
    12181 gcgaagtaga gcctgagtaa agccgtttcc agtttgctgg aggacgatta atcggtacga
    12241 ggtttccttc atcatcaaac aacgctacgc caatattggt ggctatccct ggcccctctc
    12301 ccacggaaag cacatccgga tttttaccat ccgcgacacc gtgaaacgcc acacccacac
    12361 gttcactcac caccgtgcta cattcccgta aatgaataac aaaaggcacc ggtgcgctat
    12421 cttcccctac cgcatgaaac cggttactgc tgatttgccc catattgacc gtcatttgtt
    12481 tatcaccggc ttcaatcagg caagtttccg caataatgac gccctgaaat tgcatatttc
    12541 cgccgggcgg cgtggtattc catttatttc cggccagagc aaacattggc agcaacgacg
    12601 ctaatagaaa taatctcttc cttttcatcg ttatttttat cgcacaaggg tgggcatccc
    12661 tgcccgtaat gacgtccctg aacctgggta ggttattgat actgaacctt gaaggtcgca
    12721 tccgcattag cagcacccgg ggttgcctcg ccgattgcat aataacgcgc ctggaacgga
    12781 atggtgttag taccgttatt cagggttgtt tgctcactga atgtcgcacc atccagcgtc
    12841 agcgcagcac ccgttctgtc caggatctgc acaccaacgt ttgttgcgct acccgcagct
    12901 gaactctgca gagccagaac gttggtatga cccgcatcaa tcgccgtacc taaaaaggca
    12961 acagcggctt tagatgcaac attggtatcg caatcattca gctgaatgtt aaaaccgaca
    13021 gcagaactgg ttgctccgtc ctgtgccagc gatgcggtac gaacctgtcc taactgaacg
    13081 gtttgatcaa cagagcctgc atcaactgcg caagcggcgt taacaacttc ccctttaaag
    13141 tgaacggtcc caccattaac cgtcgtggca gcggccagag ccgctgtaga actgagggac
    13201 agagccgaca gaacaacgat tgccagagtt ttaattttca tgctgctttc ctttcaaaaa
    13261 actatttcta aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga
    13321 ctgccaacac tgcacagttt tcccccaaaa gatgaaacat ttggggccat tttgactcat
    13381 agaggaaagc atcgcgcaca aactttttca gtttatttgc tggattaata ttctattgtt
    13441 atctttattt atagattttt atattgcatg aggtggtttt tggagagaag aatgaggaag
    13501 a
//
LOCUS       DAZFTN010000123        13371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFTN010000123 DAZFTN010000000
VERSION     DAZFTN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            121..1179
                     /locus_tag="ACHKV2_004057"
     CDS             121..1179
                     /locus_tag="ACHKV2_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO6941849.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1189..2331
                     /locus_tag="ACHKV2_004058"
     CDS             1189..2331
                     /locus_tag="ACHKV2_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO6941850.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2324..3097
                     /locus_tag="ACHKV2_004059"
     CDS             2324..3097
                     /locus_tag="ACHKV2_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941851.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3099..4178
                     /locus_tag="ACHKV2_004060"
     CDS             3099..4178
                     /locus_tag="ACHKV2_004060"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO6941852.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4178..5134
                     /locus_tag="ACHKV2_004061"
     CDS             4178..5134
                     /locus_tag="ACHKV2_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO6941853.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5145..6353
                     /locus_tag="ACHKV2_004062"
     CDS             5145..6353
                     /locus_tag="ACHKV2_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO6941854.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6371..6838
                     /gene="terW"
                     /locus_tag="ACHKV2_004063"
     CDS             6371..6838
                     /gene="terW"
                     /locus_tag="ACHKV2_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO6941855.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7099..7428
                     /locus_tag="ACHKV2_004064"
     CDS             7099..7428
                     /locus_tag="ACHKV2_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6941856.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7415..7756
                     /gene="tnpB"
                     /locus_tag="ACHKV2_004065"
     CDS             7415..7756
                     /gene="tnpB"
                     /locus_tag="ACHKV2_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6941857.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7838..8668
                     /locus_tag="ACHKV2_004066"
                     /pseudo
     CDS             7838..8668
                     /locus_tag="ACHKV2_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8699..10312)
                     /locus_tag="ACHKV2_004067"
     CDS             complement(8699..10312)
                     /locus_tag="ACHKV2_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO6941858.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10343..10693)
                     /gene="tnpB"
                     /locus_tag="ACHKV2_004068"
     CDS             complement(10343..10693)
                     /gene="tnpB"
                     /locus_tag="ACHKV2_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6941859.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10690..11115)
                     /locus_tag="ACHKV2_004069"
     CDS             complement(10690..11115)
                     /locus_tag="ACHKV2_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6941860.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11169..11920
                     /locus_tag="ACHKV2_004070"
                     /pseudo
     CDS             11169..11920
                     /locus_tag="ACHKV2_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            12260..12433
                     /locus_tag="ACHKV2_004071"
     CDS             12260..12433
                     /locus_tag="ACHKV2_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941861.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
BASE COUNT         3207 a         3526 c         3493 g         3145 t
ORIGIN      
        1 actgacgcac tccataacca caggagttat taatcatatt atgattgaca tgtagcttag
       61 acactcatag actcacttct caatcataaa tttgcatggc gttccggata attaaaaaaa
      121 atgaacaaga aaatttggtt tatggaaggt ttatcctccc agcgagatat tattcagggg
      181 gtaaaatcat ttgcacaaaa aaataatttt gccattaccg tttttgcctc ccaccgtaac
      241 gaaagaaatg aaatcctttc cgttgccgat tattctttga ctgaacctga agatcctcaa
      301 aaacgtcttc agtttatcca ggaaaccatt cagacctacg gcatccacca tattcatact
      361 ggccgtaaca gccagtggtt tgaagaacac cgttcagcca ttgaaccgac cggtgccacc
      421 cttactaccg gtgcaacggg cgtcgactgg ttaactctgg ctgacgaaaa agttactttt
      481 gctcagttta tggagcaaaa gggtctcccg gtcgtaccat cctggcgggt gaatacgctg
      541 gcagaattaa agacacacct cgcggccccg ccgttcactg acagcccggt atgcgtgaag
      601 ccggtgacgg gtatctatgg catgggattc tggcgctttg atgacagtgc ttcgcctatg
      661 gccgtcttta atcatcccga acatcgtctg gtcagtccgc aacagtatat tgcagcagca
      721 tcagctgctg agtcgtttaa accccttgtt ttgatgccgt acctgccagg cccggaattt
      781 tccgtcgata tcctcgcgga taagggcgaa atactcgcag ccgtgggacg ccgtaaggaa
      841 ggggctatcc agtatctggt aaacgaagga agcgcctggg aactggcgtg tgactgcgcc
      901 cgtgttatga aggccgacgg gctggtgaat gttcagacgc gaaacgatgt gaatggcaac
      961 ccggtgctgc ttgaaaccaa catgcgtccg tcaggggggg tgggttatac ccttcacagc
     1021 ggtgtgaacc ttcctgggtt atttgctgcc tttaagctcg gtctgatgtc tgaagatatg
     1081 gtacgccaga gcgctaaaaa caccttttct ccggttgcgg tgagatccat tacggatgta
     1141 attgcatacc cggaatcact ctctaacctt ctgaattaag gcgtatttat gtctgatatc
     1201 ctcgaagata tgatttatcg ccgtaccctc tcatgcggta cgattcaggt aacccgtgac
     1261 cagggtgaag tttcgctcga tgacctgttt gacatcgctg aaagacgtaa cccgaaacgc
     1321 gcctttctgt ttgtcagcaa agtactcggc aggcacattc ccgtgccacc ctcagtcatg
     1381 cggcaggcct acagacagct tgccagccag ttcccctcga cactgacagg acccgtactg
     1441 tttatcggta tggctgaaac cgccgttggg cttggggccg gtgtatttga tgaagtgcgc
     1501 caccagcatc gtgaatctgt ctatctgacc tctacccgtc acccggttga tggcacgttg
     1561 ctttgcgagt tcaaggaaga acacagccac gccaccgatc atctgatcta tctgccagat
     1621 gatgaagaga aaagacgtcg tgttaccaac gcacgaacgc tggttttgat tgatgacgag
     1681 gcaaccaccg gtaatacctt tattaacctg ctttctgccc tgcgtaatac cggcaagctt
     1741 caacatattg aacaggttat cgccgtaacg ctgaccgact ggagtggcaa tgccctgtcg
     1801 gagcgcaccc ccttgcccgt cacttcggtt tcgcttgtaa gcggtcagtg gggatggacc
     1861 ccattacctg atgcacctgt cccggacatg ccgaaagtca acgtaacttc acgaggagaa
     1921 tgggacatcc aggggaaaca gtcatggggc cgactgggga tgctcgcacc tgcggccgat
     1981 ctcggccatg aggtctccgt ccacaagggg gaacgtattc tggttctcgg gaccggggaa
     2041 ttcgtctggg agccgttcct gcttgctgaa cggctcgaag ctgccggagc acaggcattt
     2101 tatggatcga ccacccgctc ccctgtcgcc gttggttatg ccattgagtc cgccatttcc
     2161 tttacggata actacgggct gggcatcccc aattttgtct ataacgtcgc ccaccagcag
     2221 tttgaccgca ttcttgtgtg tactgagaca cccgcagaaa gtattgacac gcagcttctt
     2281 aaagcgctgg ctgaggttgc gcccgtcgtg gagattgtta cctatgaata aacccgttgt
     2341 tctgagcgac cttgacgaca cgctctttca gacccgtcgt aaaatggtgg acgagctggc
     2401 cctggagcca ttccgtaccg gtgccgttga tcgcaccctg aatccacgaa gctttatgac
     2461 ggaagaacag tccatgttgg tggactggct cctggagcag gctgaactca tacccgttac
     2521 cgcacgggga actgaagaaa tcagccgcgt acggatccct ttccactcct gggcaatcac
     2581 aacgcacggg gccgtcattc tcacgcctga aggcaaaccc gacgaggagt ggaaggccca
     2641 tatgctcggc cagctggctc cctaccagga aaagctgaca tcgatgcagc gtctgatcac
     2701 tgaaatgatg gacgcaaaag gaatcaatgc ctgggcaagg ctgaacttcg aatacggtga
     2761 aacggcggtt tatatggtga tgaaacaccg cgacagcacc cgccttgacg agctcaatgc
     2821 cattgcagat gagatagaaa cggtgtttcc gaccgagggc ttctacatcc accgcaacag
     2881 taataacgtg gcctggcttc ccaccccggt tgagaaaggg ctggcagtca gctggctgct
     2941 tgaaaaactt cgggctgaac gaggagtttt ccccgtaatt ggtttgggcg acagcctgag
     3001 cgatcatcgt tttatgaaac tgtgcagctg gtttggcatc ccgcgtcaga gtcagtttgc
     3061 agatgccatt tcacagcgaa tttttggaga aaattaaaat gcagccacat gaaacattta
     3121 ccggctcata ccagcccggt gacgtggaat ttctgctaaa gccggttgtc attgagatga
     3181 cgccggttga gaaaaaagaa gaactgattc agtccgggaa gaaacattat tcggacatgc
     3241 tcagccagga gccagcgcct acacaatggc atcttgattt gtttcaccgg gcgctggatc
     3301 ggggcgcaga gaggcttgca aaagaagtca cacagctcgc tattgctctg gccgaacgct
     3361 tcggtgatga gcccattgta ctggccagtc ttgtcagggc tggcgttccg ctcggcgtta
     3421 tgctgcacca ggccctgcgt gacatgggga aaacctcatg gcattacggt atcagcatta
     3481 tccgggatcg tggaattgac ggtgcagccc tcgacgtcat tgaagagcgg catggtacca
     3541 gcggtattgt ctttgttgac ggatggacag gtaaaggcgc aattaccgga gagcttgtac
     3601 gcgcactgaa agatcgcccg ggctatcctg agcagccgcg actggttgtc ctggccgatc
     3661 cctgtggctg ctcctggctt gcagccagtg atgatgactg gctcattcct tttggcatca
     3721 tgggggcgcc ggtatcaggc ctgatctccc ggtcagtatg gtcctctgaa ggattacacg
     3781 ggtgcatggt ttgcgagcat ctcagtgaat tcgaatgcag ccggatgctt gccgataccg
     3841 tcgctcattt ccgtaagaag ttaacaccgt catccctcgc tcccctgagc tggaatacgg
     3901 aatcagcccg ggtcttatgg cagacaagtc gcgacgttat cgcgttcctg gccgatgaat
     3961 ttaaagtgga cagcgtcaat cgtattaaac ccggtattgc tgaagcaacc cgggctgtat
     4021 tacgtcgggt accggaccat gtatttgtgc gttctattga cgacccggac gttgccttgc
     4081 ttgtagggct tgctcgtgaa aagggaatag ttgttacaga aatgggggga accctcggcc
     4141 agtatcgggc tgtaaccatt atcaagaagg tactctgatg aaaaatcgtc tttctccctg
     4201 gaatctggga gccacgctat acatgcctgc aacacgggaa gatattgctg atgccgtcct
     4261 gcacgggaaa atcccgggct tgcgctctct ggtgatttgc ctggaggatg ctgtcagtga
     4321 agcagacatc cccgtcgccc tcaaaaatct ggagcacctg ctgcacgagc tcagtaacag
     4381 catgagtagc ctgggtaaaa atgactggcc tctggtgttc attcgccccc ggcatgccga
     4441 aatgggcaga tggttaaaag cgcattatga tctttccgct gtcgacgggt ttgtgctgcc
     4501 taaatttacc ctcagttcgt tagctgaatg gtgggatatc atggtcggga ctcacctgtg
     4561 catgatgcca acgctggaaa cagaagatgt ctttgacgtg gttcagatgc gcgagctggc
     4621 tacccgtctg gaggaacatc cctgccacga ccgcattatt gcgcttcgaa tcggcggcaa
     4681 cgatcttatg aacgttgtgt cgcttcgccg tccccgggac ctgacgctgt atgacagccc
     4741 gatgggctac gtcatcaaaa tgctcgtttc ggtcttcggc ccgcgtgatt ttgccctgac
     4801 cgcgcctgta tgtgagcata ttgacgatca tgccgttatg gccagagagc tggctcttga
     4861 tatggcacac gggcttgttg ggaaaacggc cattcacccg ggtcagatag aggtcattca
     4921 aaacgcgctg atggtcactc agggtgagca ttctgacgct ctgcggatcc tgaactctac
     4981 ccaggccgtg tttaagtcgc agggagcaat gtgtgaaccc gccacacatc gccgctgggc
     5041 ggctggcatt ctggacaggg ctcgttttta tgggttacag aacgagcaaa gcgctgatgg
     5101 aatcagatta cttaccgtga cccagcatca ttaaggaaat cataatgggg ttcaggttca
     5161 gaaagtcgat caacattatt cctggcgttc gcctcaacct gagtaacggt gcaccgagcc
     5221 tgagtgtcgg gccgagaggt gcttccgttt cttttggtag ccgggggacc tatgccaatc
     5281 tgggcttgcc cggtaccggg ctgagttacc gtacccggct tgaccgggcc gcgcgttcca
     5341 gaggtgaaaa ccggacggca accgacccgg ggctcagaca ggcgcttgag cagaaagccg
     5401 ctgaactcat gtcagcggta accgcaatcc gtaatattca cgagctgacg ccggatccaa
     5461 aaacaggcat cagctgggca gagctggaag cagtatacct gcataacaga acgtcgcctt
     5521 ttcaggttcc ggcaccggtg cgtccagaaa agccagacta ccttgtattg ccggaaaagc
     5581 ctgccgagag cgaaggcatt agttttttgg gtaaatggtt tgaatcggaa tcagctaaag
     5641 ctgagcgcca cgccgaaaat cttcgccggt ggcagcagga gctgattgat gtggagcgtg
     5701 agaataccct tcgacagcac cggtaccagc aacaacggac ggcctgggcc gaacagtatg
     5761 caaactggaa gtttgaagct gaagaacatg aaaaacggct cgccacggct caggcagatg
     5821 cccggcagca gttccggaca gacgccgcgt ttttcgaatc atacctggcg ggtgtgctgg
     5881 cagaaactga atggccgcgt gaaacgcttg ttgcatttga agtaaagccg gagctatcag
     5941 cagtcctgct ggacgttgat ttagctgaga ttgaagattt ccctgataag atttacggcg
     6001 ttaatgcccg gggaacggag ctgacggaaa aagccatgac gcaaaaagcc gtacgcgaaa
     6061 actatgccca ccacgtccat ggctgcttgt tccgcctggt cggtatcgtt ttacatacgc
     6121 tacctttcga caacgtgatt gtgtcaggct ttacgcaacg ggtcagtaag cggaccggct
     6181 atctggagga tgagtatatc ctgtcctgca aatgcactcg cagccagatg tcgtcagtaa
     6241 attttgcagg cataaaacac attgatccgg ttgaagcgtt aggcgatcaa ccggttattc
     6301 gaaagatgag cagtaccttc atttttcagc ctattgaacc actaaccctt taagcaggct
     6361 aaatcttacg atgcaattaa ataccagaca ggcccggatt tttaagcttg ccaacttact
     6421 gggcaccggc aagcccgttt ccgctgccga cattataacc agtctggaat gctctgagcc
     6481 gacattaacc cgggccctca aagagctacg cgagtcatat tctgcggaga tcaaatacag
     6541 caaggcaggt cattcctatc atctcgttaa tccgggccag ttggataaaa agaccctccg
     6601 ccggatgaat gaagcgctcg cacagaatgc tgaactgaaa accggtgagt ccacagggaa
     6661 ggtcgtactc gataaggata aaaaaacagc cgtgtcatta tcgctacgca tgcggattct
     6721 aagaaaaatt gaccgtctgg ctgcgctgag tgactcgacc cgaagtgaag cggtagagaa
     6781 gctggctctg cactccgttg atgaactgat aaaagaatac agcgctaaaa agtcctgacg
     6841 ctaaatctca gaccggggtg aaaactccgg ttttgtcata tttcacctct gtattggcgt
     6901 gatgcaaaat ggcatctgaa tcgatcatgg tttggcatcc atttccgatc actgtttggc
     6961 atcacaatcg atcacgattt ggcatgcttc cgctcattga ttggcatcct ggtgtcgtta
     7021 cggggaagtc atcttcacag ggatcttcag tcagttagca ttaccgacat ccattcacca
     7081 acttaccgga gataatcaat gtcaaaaccc cgatggacgc ttgatcagaa aaaacatcac
     7141 gttgccgcat ggcgcgccag cggccttacc cgtgaacagt actgtgaact ttacgacatt
     7201 ccgtttaaat ccctgcgcca gtggccgcag gatgtcgcaa aagccgaaaa acgagccaga
     7261 gctccagaaa ttattcctgt ttcagtatct ggctcttccg gaatgactga tggcagacca
     7321 ttgtcggatg agcctgtcac tctcttcctg cccggtggta tccggatgtg ctgccagcct
     7381 tctcagttaa ccgatgtttt ccgggctctt cgccatgctg acgcctgata acgtattcct
     7441 ggtcgtaaaa cccgtcgata tgcgccgggg tatcgacaca ctgacacagt acgtccagaa
     7501 cgaacttaac gctgcatggc atgacggtgc tgcttttgtc ttcaccaata aagtccgctc
     7561 ccgcataaaa gttctccggt gggacaaaca tggcgtatgg ctgtgtaccc gacgactgca
     7621 ccggggaagc ttccgctggc cccggaaagg tgatgccaca tggcatctca cacaggatga
     7681 gtttcactgg ctggtctttg gggttgactg gcaacaggtg aaagggcatg acctggcaaa
     7741 atgggtatac cagtaagaac ccgaagatgc tcagagcaac gcaataactc aatgaaatat
     7801 aatgaatatc catgcgtgat gtcatataat ccaccgcatg gatatctcgc ttctcagtac
     7861 caccagtgac cctgaacagc ttcgcgctct ggcaattgcc atggtgcaaa aagtcatggc
     7921 tgagaatgca gaacttcaga accgtatccg catcctggaa gagcagatga agctcgcacg
     7981 ccagcagcgt ttcggtaaaa aatgcgaatc gctggccggg atgcagcgtt ccctgttcga
     8041 agaggatgtt gacgcggata tcgcagaaat ctcggcgcat ctggacaaac tgcttcctca
     8101 gaccggggac gaagaaaaaa caacaacacg tcctgttcgt aagcccttgc cttcacctct
     8161 tccccgtgct gaaaaggtta tcccgccagc tgaggaacgt tgtcctgact gtgatgcccc
     8221 cctgcacttc attcgcgatg aagtcagcga gaagctggaa tatattccgg cacaggtcgt
     8281 ggtcaaccgc tatatccgcc cgcagtacag ctgtccctgc tgtgagaaag tgttcagcgg
     8341 aaaaatgccg gcacatatcc ttccgaaaag tgctgttgag ccatcggtca tcgcacaggt
     8401 tgtcatcagc aaatataccg accatctgcc cctgtatcgc cagcagcaca ttttctcccg
     8461 aatgggggtg gagctccccg tcagtacaat ggcggatatg gtcggtgttg ctggcgctgc
     8521 actggccccg ctggcaaaat tgttacgcca cgagcttctg acacgggatg ttattcatgc
     8581 agatgaaacg tccctgaggc tccttgatac ccggaaaggc ggtaaatcct gctccggctg
     8641 gctgtgggct tacgtcagcg gagaaagagt aagcgtcaac ggagcaccgt attgacgctt
     8701 atttattggt gagtactacg ttccatggca ggagttcatc aactcggttg gaaggccatt
     8761 ctggcagtac gctcaggata tggcgcagat acgcttccgg atcgataccg ttcagacggc
     8821 aggtgccgat cagcccgtac aacagtgctc cacgctcgcc gccgtgatcg ctgccaaaga
     8881 acataaagtt tttctttccg agacagactg cacgaagcgc tctttccgca gcattattat
     8941 ccgcctccgc cagaccgtca tcactgtaat aacagagggc atcccactga ttcagtacat
     9001 agctgcacgc ttcgcccagt ctggattttt tcgacagcgt accattcttc tccaccatcc
     9061 attcatgcag tgacgtcagt aacactttgc ttcgctgctg cctgacggca agacgctctg
     9121 actccggtaa tccccgtatt tcatcctcga tggcgtacag ttcactgatt cgcttcaggg
     9181 cttcttctgc cgtcgcactt ttgctgctga tgtatacatc gtggattttt cgccgggcat
     9241 gggcccagca cgcaacttct gtcagtgcac caccttcacg ttctgcactg aacagccgat
     9301 cgtaaccgct gaacgcatcc gcctgcagga taccccggaa gggacgaagg tgttgtaccg
     9361 gatgttttcc ctgcctgtct ggtgagtagg cgaaccagac cgccggtggc tctgatgagc
     9421 ccgcattccg gtcatcccgg acatacgtcc agatgcgtcc tgtttttgcc ttttttctgc
     9481 ccggtgccag cacttttact ggtgtgtcgt cagtgtgaac cttgcgggtg ttcatcacgt
     9541 aacggtacag ggtatcattc agcagagtca ttaactgaca acatgcgtca acccagttgg
     9601 agagtaatgc ccggctcagt tcgatgccct ggcgggcaaa gatttcactc tgacgataca
     9661 gtggcaggtg ttcgcagtat tttcccgtta acacgcgggc aagtaatccg gagcccgcga
     9721 tgccgcgctc tatcgggcgg gacggcgctg gcgcttcaac tatacagtca cattttgtac
     9781 aggctttttt tacccgaaca gtgcggatca ctttcagggc gctactcacc agttccagct
     9841 gctcagcact gacttccccc agataatcca gctcactgcc acactccggg caacaacttt
     9901 cttcaggctc cagacggtgt atttcacggg gaagatgtgc cggtaacgga cgacgatgac
     9961 gtgactgtcg caactggcgg ggaacctgcg ggtcatcctc acgcccactg taacgatcgc
    10021 tttcctgttc gcgttgtttc agttgggcct cagcctgttc aacctcacgc tgcagttttt
    10081 cagaacgggt accgaacaac atccggcgca gtttttctat ctgggccctc agatgttcta
    10141 tttcccgctc ctcctcttcg atcttttcct cggcacgtgc cagtgcagag cgcaggaagg
    10201 cctccgtctc ttcaaccaga ctcagttgct gatctttctg acggagggct tcagcctgct
    10261 cagagagcag cctttccagc tcagtgatac gaatgaggta tttccgactc atgaccgttt
    10321 ttataatccg gccatgacat ttttacaaca ttgtcagtgc attaaggcgg gatgttttgg
    10381 gctgacgcca gtccagttta tcgaggagca ttgccagctg cgagcgggta atggatacct
    10441 taccgtcacg caccgcaggc cagataaact ggccttcctc cagacgtttg gtgaacaggc
    10501 acagaccatc agcatcagcc cacagaattt taatcgtgtc accccgtcgg ccgcgaaaga
    10561 taaacaggtg accggagaag gggttctcat ccagcacatg ttgtacctgt tctcccagac
    10621 cgttgaagga tttacgcata tcagtaacgc cggcaaccag ccagattcga gtgcctgatg
    10681 ggagcgagat catcgtcccc tcccggtcag ttcacggatc aacaccgtga gcagctctgg
    10741 tgaaggattt tccagcgtca tgttaccgtg gcggaactca actttacagg aactggcact
    10801 gactgtgctt tgtgaaggag tggataaaag cggagtaaga gccgccatag gctctttctg
    10861 ctcatcaggc gttatctcaa caggtaataa ttcaacgcca gcgccagaag aggttgttac
    10921 cggaagacgc cgcgatatac gcctttcgtt ctgccagagc ctgagccatt tgaacaggag
    10981 gttatcattg atatcgtgtt cccgggcaat acgggcaaca gaggctcctg gttgtgaagc
    11041 cagtttaacc atttgaagtt taaactcatt tgaaaatgat ctgcggggtt ctgaggataa
    11101 tattttctgt tccataacag gtgtccacta gttgaaaagg tgggcaccta cgttaccaat
    11161 actggcttaa tggctacata cggcggtcag tttacgctta cgagaaagaa gcggtccgcc
    11221 ggtcgtctgt tttgactcgc agaccggacg ggccctcaga tatcctgagg cctggctgca
    11281 gggctggtgt ggcggtacgc tggtgagtga cggatacagc gtgtacaaat ctctggcgga
    11341 taatgacccc ggtattacat ctgcctgttg ctggagccac gcaaggcgcg gtttcgcaaa
    11401 tctgtataag gccagcaggg agccccgtgc agccatggcg ctgagaaaaa tcgcgggtct
    11461 ttatcgcatt gagaagttca tccgtgaacg tcctgtggaa aaaatacggc aatggcgaca
    11521 acggtattcc cggccgatag tgaatgacct gtttgcctgg cctgaagagc aggagccgtg
    11581 ctgtccgccc gacggaccac taaacaaagc gattaattat atcctgaacc ggcgcgatga
    11641 actgagctgc ttcctgagtg acggagccgt gcccctggac aacaatatct gcgagcgtgc
    11701 tatccgcccg gtggtcatgg ggcggaaagc atggttattc gccggttcac tgatggccgg
    11761 gaaccgcgcg gcacagataa tgagccttct ggaaacggca aaacgtaatg gtctggagcc
    11821 gcatgcctgg ctgacagacg ttctgacgcg tctaccggag tggccggaag accgactgga
    11881 agagttgctg ccccttgagg gatttacctt ctccgggtga gtgatacctg ccatcaggtg
    11941 ttcgtgcagc gggccataac ccgcagtcgg gagttgaacg cctgacgaca ggaaatgagc
    12001 cagagcagcg accacgcggg ctgacagtga gtcagaacct gaatcagccc ggagccattc
    12061 ccggggctgt acttcatcac ctgccatcag tacccgtgtc agtacttcgc gacctgacgc
    12121 aattacccga ctcagttctt cagaacccgc ctccgatacc agcctggcgg tcgcccctgt
    12181 gcaacaaaat aaatcaacca tcgtcagacg gtgatcaatc gtatgcgctg ccagtggctc
    12241 gcctgaaaaa tgcgtcaata tgagttcgcc ggaacaatac ctcagttctt cagaacccgc
    12301 ctccgatacc agcctggcgg tcgccccggc gcaacagaat aaatcaacca tcgtcagact
    12361 gcgatcattc gtatgcatcg ctgatgatgc gcccgaaaaa cgcgtcaata tgagttcgcc
    12421 ggaatattac tgacgacacg gtttgtagtt acgttccttc ggtgagtgag tacctgtgcg
    12481 tcagggtaac gcaatcagtt caggtttagc ggggtgtcaa gtcggtttgg ctaaccgacc
    12541 gcagaattaa ctgtaccttt aaaggtcgcc cccgatatat ctgcctgtca gacatatcgc
    12601 cgattcggta tctcactaca taaaactcct taaaaaacaa tgcgattctg ttggtacgaa
    12661 gctttcagta agtcagtccg gagaagcgcc agtacgatac agaatgaggt tgaaaaaaac
    12721 gttcacgtct ggtcacttct tcaaatgtta aaaaacatct cgtaaagagc cagtttatgc
    12781 gggttcgttg agtagacgtg cgagcgtcac tttgtcagct ttaacgctga cgaaagtgac
    12841 gcccgcacag cttcaaactc cacatgcaca cgcaagcgac caaatttcaa atggtcttat
    12901 gccgtgcgca cagtcataag aagcgcatgc ggaaagtcag aaaatatctt aagacgcaga
    12961 ttttaaaggc gtttttatgc cagtctgccg ggaatacaca gtagtccggt tgtccggaag
    13021 caccacggta atccgaagga aaaatcaacg tacctcaatc accgtagtga tatccgttcc
    13081 cagaggctaa acggatgtac caccataaag gcatcctctt tcaggctacg aggatattgc
    13141 aatcacccga aggcgtttct ctcagattgc tgaaacattg gctggcagtc acccgaaggc
    13201 gtccctctca gactgctgga acattggctg gtcgcaaaag cgacagttaa tgcaaataca
    13261 ggttaacaaa aagggaaatc tgccgtcaac ccaaccatat gaacatgttg atttcattat
    13321 gaatgtgatg ttctaatata aattaatcag taacttcata aaaagaatat a
//
LOCUS       DAZFTN010000124        13170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFTN010000124 DAZFTN010000000
VERSION     DAZFTN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            289..2526
                     /gene="nrfB"
                     /locus_tag="ACHKV2_004072"
     CDS             289..2526
                     /gene="nrfB"
                     /locus_tag="ACHKV2_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO6941862.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2513..5485
                     /gene="nfrA"
                     /locus_tag="ACHKV2_004073"
     CDS             2513..5485
                     /gene="nfrA"
                     /locus_tag="ACHKV2_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO6941863.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5486..6376
                     /locus_tag="ACHKV2_004074"
     CDS             5486..6376
                     /locus_tag="ACHKV2_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO6941864.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6559..7320
                     /gene="envY"
                     /locus_tag="ACHKV2_004075"
     CDS             6559..7320
                     /gene="envY"
                     /locus_tag="ACHKV2_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO6941865.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7366..7442)
                     /locus_tag="ACHKV2_004076"
     tRNA            complement(7366..7442)
                     /locus_tag="ACHKV2_004076"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7406..7408),aa:Arg,seq:tct)
     gene            7685..8317
                     /gene="fimZ"
                     /locus_tag="ACHKV2_004077"
     CDS             7685..8317
                     /gene="fimZ"
                     /locus_tag="ACHKV2_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO6941866.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8320..8835)
                     /gene="sfmF"
                     /locus_tag="ACHKV2_004078"
     CDS             complement(8320..8835)
                     /gene="sfmF"
                     /locus_tag="ACHKV2_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO6941867.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(8846..9853)
                     /gene="fimH"
                     /locus_tag="ACHKV2_004079"
     CDS             complement(8846..9853)
                     /gene="fimH"
                     /locus_tag="ACHKV2_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6941868.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(9866..12475)
                     /locus_tag="ACHKV2_004080"
     CDS             complement(9866..12475)
                     /locus_tag="ACHKV2_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO6941869.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12506..13168)
                     /gene="fimC"
                     /locus_tag="ACHKV2_004081"
     CDS             complement(12506..13168)
                     /gene="fimC"
                     /locus_tag="ACHKV2_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6941870.1"
                     /translation="MIFYLIISASVHAAGGIALGATRIIYPADAKQTAVWIRNSHTNE
                     RFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVK
                     TIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNP
                     SPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVN
                     LN"
BASE COUNT         3422 a         3337 c         3170 g         3241 t
ORIGIN      
        1 ttgcggggag cgggctttcg taatcttgcc ctgcctgctc acctccaggc acttccacta
       61 catcatgacc tgatcggtgc caccactcct cttcactatg acgttaacct cccctccttg
      121 acaccacgct tttgtctaca ttctacgcaa acggcaactt ttataaccag tgtaaaaata
      181 acgtgccgca atagtcatac ctattaatgg taaaaagctg tcacaattca taaaaaacct
      241 taatatacgc caccctaaac ataaccagcg ttaatgtaag gtttttgtgt ggactggctt
      301 cttgatgttt ttgctacctg gctctatggc ttaaaagtaa tcgcgataac gttagcggtc
      361 atcatgttca tcagcgggct ggacgatttt tttattgatg tcgtctactg ggtacgccgc
      421 attaaacgca agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat
      481 aaaccagatg aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac gggcgtcatc
      541 ggcaatatgg ccgagctggc ggcgaccacg ctcgactacg aaaactatca tatctttgtt
      601 ggcacctacc ccaacgaccc ggacactcag cgtgatgttg acgaagtgtg cgctcgcttc
      661 ccgaatgtgc ataaggtagt ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg
      721 aacaacgtgc tggacgccat cacccagttt gagcgtagcg ccaatttcgc ttttgctggt
      781 tttattctgc atgacgccga agatgtgatt tcaccgatgg aattgcgtct gttcaactat
      841 ctggtcgagc gtaaagatct gattcagatc ccggtgtatc cgtttgaacg cgaatggacg
      901 cacttcacca gcatgactta cattgatgag ttttcagagc tgcatggcaa agatgttccg
      961 gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg tttcagccgc
     1021 cgcgccgtga ccgctctgtt agctgacggt gacggtattg ctttcgacgt gcagagtctg
     1081 actgaagatt acgacattgg cttccgcctg aaagaaaaag gtatgacgga aatttttgtc
     1141 cgttttccgg tggtggacga agccaaagaa cgcgagcagc gtaaattttt acagcacgca
     1201 cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg ataccttttc gactgcggtt
     1261 cgacaaaaat ctcgctggat catcggcatt gttttccagg gctttaaaac ccataaatgg
     1321 acctccagcc tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc aatcagtaac
     1381 tttgtcagct tcctcgcaat gctggtgatg atccagcttt tgctgttgct ggcgtatgaa
     1441 agtttgtggc ccgatgcctg gcatttcctt tctattttta gcggcagcgc atggttaatg
     1501 accctgctgt ggctaaactt tggcttgatg gttaaccgca tcgtgcagcg ggtgattttc
     1561 gtcactggct actacggcct gacgcagggg ctactatctg tcctgcgtct tttctggggc
     1621 aacctgatta actttatggc caactggcgc gcgttaaaac aggtacttca acacggcgat
     1681 ccacgtcgcg tcgcgtggga taaaacaacg catgacttcc ccagcgtcac tggcgatacc
     1741 cgctcgttgc gcccgttagg tcaaattctg ctggaaaatc aggtcatcac tgaagaacag
     1801 ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg
     1861 caggggctga ttagcgccga gcagctggca caggcgctgg cagagcaaaa cggcgtggcg
     1921 tgggaatcca tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat gccggcctcc
     1981 gtggcgctgc attatgcggt actgccgctg cgtctggaaa atgatgagtt aattgtcggc
     2041 agtgaagatg gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa
     2101 gtgcgttatg tcattgttct gcggggacaa attgtcacgg ggttacgtca ctggtatgca
     2161 cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca tcagtggctc
     2221 acggaacagc aggccggtga aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc
     2281 gaaatactga ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc
     2341 catgaacgca gttctctgcc gctcggcaag tttttggtca ccgaaggcgt tatcagccag
     2401 gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac aagtttcgat gcaatcacta
     2461 ttactcaaag caggtttaaa cacagaacag gttgcgcaac tggagtccga aaatgaagga
     2521 gaataacctt aatcgcgtca tcggatggtc tggtttactg ctgacgtctt tattgagtac
     2581 cagcgcactc gcagacaata tcggcaccag cgcagaagag ctggggctga gcgattatcg
     2641 ccattttgtt atttatcccc gtctcgataa ggcgctgaag gcacagaaaa ataacgacga
     2701 agcaaccgcc atccgcgaat ttgaatatat acaccagcag gtgccggata atattccgct
     2761 gactttatac cttgcggaag cctatcgcca ttttggtcat gatgaccggg cacggctgtt
     2821 gcttgaggat caactgaaac gtcacccagg agatgcccga cttgagcgca gtctggcggc
     2881 tattccggtt gaagtgaaaa gcattacaac tgttgaagaa ctgcttgccc agcaaaaagc
     2941 gtgcgatgct gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct
     3001 ggcacagtta cctgtcgcca gagcgcaact gaacgacgcg acgtttgctg catcgccgga
     3061 aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc tacctgaaac aatggtccca
     3121 ggcagatacg ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg
     3181 tcagtggttt gacgtgcttc ttgccgggca gctggacgat cggatcctgg cactgcaatc
     3241 acaggggatc ttcaccgatc ctcagtcata tattacttac gcgaccgcgc tggcttatcg
     3301 tggcgaaaaa gcacgcctcc agcattatct cattgaaaat aagccgctgt ttaccacgga
     3361 cgcacaagag aaaagttggc tctatctgtt atctaaatac agcgccaacc ccgttcaggc
     3421 attggcgaat tatacggtgc agtttgccga taaccgccag tatgttgttg gcgcgacgct
     3481 accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc
     3541 cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta acaaggctga
     3601 agctctgcgt ctggcacgct tgctgtatca gcaagaatcg gcaaatctta cccgcctgga
     3661 tcaactaacc tggcaactga tgcagaacga gcagtcacgg gaagctgccg atttattgct
     3721 gcaacgctat cctttccagg gcgatgcgcg tatcagccag actttaatgg cgcgactggc
     3781 gtctctgctg gaaagtcatc cttacctggc aacgccggcg aaggtggcga ttttatcgaa
     3841 acccttaccg ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa
     3901 ttgcccggca atagttcgct tgctgggcga tatgtcgcct tcctacgatg ccgccgcctg
     3961 gaaccgtctg gcaaagtgtt atcgggacac gctacccggt gtggcgttgt atgcatggct
     4021 tcaggccgaa caacgacaac cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta
     4081 tcaggttgag gactacgcca ccgcactggc ggccaggcag aaaatcagtc ttcacgacat
     4141 gagcaatgag gatctgcttg ctgctgccaa taccgcccag gcggcaggaa atggtgcggc
     4201 tcgcgatcgc tggctacaac aggcagaaca acgtggactg ggaagcaatg ccctctactg
     4261 gtggctgcat gcgcaacgtt acattcctgg tcagccggaa ctcgcactga acgatctcac
     4321 gcgctcaatc aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga caatttatcg
     4381 ccaacgtcat aatgtcccgg ccgcggtgag tgatttgcgc gccgcgctgg aactggaacc
     4441 gaataatagc aacacccagg cagcgcttgg ttacgccttg tgggatagcg gtgatatcgc
     4501 acagtcgcgg gaaatgctcg aacaggcgca taaagggcta ccggacgatc cggcactgat
     4561 ccgacaactg gcctatgtga accagcgtct ggatgacatg cctgcgacgc agcactacgc
     4621 ccggctggtg attgatgaca ttgataatca ggcgctgata accccactga ccccagagca
     4681 aaatcagcaa cgcttcaatt tccgccgtct gcatgaggag gtcggtcgcc gctggacgtt
     4741 cagtttcgat tcttccatcg gcttgcgttc cggggcaatg agtaccgcta acaataatgt
     4801 tggcggcgca gcgccaggga aaagctatcg tagctacggg caactggaag ccgaataccg
     4861 catcggacgc aatatgctgc tggaaggcga cctgctctca gtttatagcc gcttctttgc
     4921 cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct
     4981 gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc agttgccgct
     5041 gaacgggcaa aatggcgcat ccgataccat gctgcgcgcc agcgcctcat tctttaatgg
     5101 cggcaaatac agcgacgaat ggcacccgaa cggttcaggc tggtttgccc aaaacctgta
     5161 cctcgatgcg gcgcaatata tccgccagga tattcaggcg tggacggcag attatcgcgt
     5221 cagctggcat cagaaggtag ctaacggaca gactattgag ccttacgctc acgttcagga
     5281 caacggctat cgtgataaag gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg
     5341 gaatatctgg accggcgaga cgcactacga cgcctggccg cacaaagtca gtctcggcgt
     5401 cgagtatcaa cataccttta aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt
     5461 tctcaccatt ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc tgacactgct
     5521 tttggtcagc cctttttcct ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga
     5581 tagtcaggtt accgataccc agtggcaggg gctgatgagt cagttacgtt tgcaaggctt
     5641 cgataccctg gttttgcaat ggacccgtta cggcgatgca ttcacccagc cagaacagcg
     5701 cgcgttattg tttaagcggg ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg
     5761 gctgaacgcc gatccggaat tttttatgca ccagaaacag tcatccgcgg cgctggaaag
     5821 ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc agattatgga gtgccgtacc
     5881 tggcgtaacg ccggatggct ggtacttcag cgctgaaatt gacgacctga actggcgcag
     5941 cgaagccgcc cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc tgattagcga
     6001 tgtttcagca aaaccggttt atatcagtag ttttttcgcc ggaaacatgt cgccagatgg
     6061 ctatcgccaa ctgctggaac acgttaaagc aaccggcgtt aatgtctggg tacaggatgg
     6121 cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga
     6181 ttgccaaagc tccgccccag caagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg
     6241 caaaaccttt acagcgaaac cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg
     6301 ttcctcttgc ggtaaagaca ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg
     6361 cattctcgag tattaaatct cctccaggta agtcgggtac gacctggctt acctctttcg
     6421 ctctcgtcag aattccatca agattaattg ctaaaaagcg tgctaaagaa agaagatcgt
     6481 tatcggcgaa cgggccgatc actcatgcag gaagcataga tcctttgagc ggagtttctg
     6541 atatctgagg agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt gatcctgacc
     6601 gaaaaagagg tagaggtaag cgtcaataac catgctacgt ttacccttcc gaaaaactac
     6661 ctggccgcct tcgcgtgcaa caataacgtc attgaactct caacgttaaa tcacgtatta
     6721 atcacccaca tcaaccgtaa catcatcaac gattatctgt tgtttttaaa taagaactta
     6781 acctgtgtaa agccctggtc gcggctggct accccggtta tcgcttgtca tagccgtaca
     6841 ccggaagtgt tccggctagc cgccaaccac agcaagcagc aacccagcaa accctgcgaa
     6901 gcggagttga cgcgcgcatt gctttttacc gtattgtcta actttcttga gcaatcgcgg
     6961 tttattgccc tactgatgta tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt
     7021 attcaaagcg atattcagca ttactggaat ctgcgaattg tcgccagttc gctatgttta
     7081 agccccagcc tgctcaaaaa gaaattaaaa aacgaaaata ccagctatag ccagattgtc
     7141 acagagtgtc gtatgcgtta cgccgtacag atgttattga tggataacaa aaatatcact
     7201 caggtggcgc aattatgtgg ctatagcagc acgtcgtact ttatctctgt ttttaaggcg
     7261 ttttacggcc tgacaccgtt gaattatctc gccaaacagc gacaaaaagt gatgtggtga
     7321 agggcaaagc ggaaacggat aagacgggca taaataaaga agaaatggcg cgccctgcag
     7381 gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg agctaagggc
     7441 gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca
     7501 atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc tgatttattt
     7561 cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt
     7621 catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta tgagactggt
     7681 aactatgaaa ccaacgtcgg tgatcattat ggatactcat cctatcatca gaatgtctat
     7741 tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg atgattaccg
     7801 cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg atatagactt
     7861 gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga gcacagtgaa
     7921 agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga tacaagctgg
     7981 tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg ccgttcagat
     8041 gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa aaagcaataa
     8101 atgtagtacg aatagttcaa cggtcactgt gctatctaat cgtgaagtga ccatattacg
     8161 ttatctagtt agcggattat ctaataaaga aattgccgat aagttattac ttagcaataa
     8221 aacagttagt gcgcataaat ctaatattta tggcaagcta ggtttgcatt caattgtaga
     8281 gcttatcgac tacgccaaat tatacgaatt aatataatat taattataat tgatcataaa
     8341 tatcgcatcc gctttcgcca cacctggccg aacaccgctg gctaacgctc gataattggc
     8401 aaaaaagttt agtgtgacat tgccatttgc atctacttcc tcagtcgggc tcgcctcccc
     8461 cagtgcgagc cgggagcgat cgctattacg taattcgatg gcgacggttt gtgccattgc
     8521 gggatcatcc agagccagca ggttggcatc agatgccggc gttcccgtaa ataaaatcgc
     8581 aactgaaccc ggaggacatc cctccagccg caggctaaaa gggaggagtg ccgtggtatc
     8641 gccagcgttc agtaattgtg tcgtaggcca tctgcctaaa tctaccgtct tatcaatatc
     8701 cgctgtgttt acggtacagg agaaatcaac aacgttaccg tgcaaattga tattaatcgt
     8761 tcctaaaggg tcagctgccc atccactgga actccacagt aacccgcaga aacagctaaa
     8821 gaatactctt ctcattctcc gtacctcatt gataatcgac gcgtaaatac cccagcgcgc
     8881 taaacggccc ttcggtcggt ttttgaccgg taatactgat aggccaggcg cgaagtgtga
     8941 cattggctgc cgcagctgca tccagacgga aaggaataac gctattgaga tcgttaggcg
     9001 tgatcggcgt atcgttctga tcggcgacaa taaaacctaa atcctgattg tccgacacca
     9061 tcgcctgacc agaaacggca ctggcttcca gacgcattgt taaataagcc tgcgcagcaa
     9121 cattcgtaca tttgaccgca atgctcttgg tttgcggcat gacaccagca ggccgattac
     9181 ccggccctgc cgcactaaat aacgatgcgc cgatatcacc aaaatcaaat tcaacaatct
     9241 gcccggcatt taattcgcag ttttgcggta cttcaacccg gccaccaaaa ctaatggtat
     9301 aaacaggtgt aaccaacgga tcgtaaggcg ttgatgtcac atacacggta aacatcacct
     9361 gtctggggat ctccaccatg ttaataaagg gccgaataac ctttaaacga aatatcaaac
     9421 gagaatccat caccccgaac ggaaggtttt gcgaaacgtt aggatctgtt cccatacgaa
     9481 tataattttg cgggggaaat tgaatatcca tcacactatc aaccagactc atcgcaccta
     9541 atagataatc atttaattgc atatatttat aattatcgat agtttcctga acaataaagt
     9601 tagtgacata actacggtat gtataattca ccagcgtgcc gggtggacaa attgctgaga
     9661 cacctaccca gccggatttt tccggtaaaa caacaatctg ccctggctgg ttattggtgc
     9721 tattaaagac atttgagaga tcatagtgtt catcttctgc tacgccagta ctatttgcac
     9781 aaacagtagc ccaggatatg tttgccagac acaaacatgc catcgccaat aataatctgc
     9841 agattatctt cattgataat ttcctttatg agccaggatg tgtgcaaact gccgaaataa
     9901 cagtgacggc ttgctgtaag ctttgcttcg gcaatacata gtgcgcaatg cagcgtgagt
     9961 tcgcgccgtc tccccactga accagtaatt caccagacaa tggcgcacca cttaaataaa
    10021 cttgcccgtc atcacccacc atactggtta ttccggtact gttttcccgt accagtgatc
    10081 caaacggcac cggttttccg ccctgggtaa cggtaatgag cgcccgcacg cctatgcggg
    10141 tatcaaaatt ggcacgaacc aacgcgcctt gcgtcggcac aacgctgcta atattttttt
    10201 caacatcgat ggaattcccc atcgtattgg tatcaagcgc gatacggtta taccgataaa
    10261 ccgtggcata cggcatcacc gcatagccgc gccagtcggt taaaatgcca gtttgatttt
    10321 caatgcgtac accgcctgcg ccaggcgctt taatcaaaac attggtatcc cctaaaggct
    10381 ggctcagcgt tatgccattt tcatgtccga ccacaccgcc agacagctgc cagttaacgt
    10441 catgttgatc gcggtcgtag ttatacccga cccccagcgt accgtaagcg gcctgccagt
    10501 ttgccgtggc gctgcccgta tacccattat tcgaggtatc accctggctt acgtgataac
    10561 tcaggttgtg gccttccagt aaggtcccac ctacacctgc cagccagcta ttttgcccgt
    10621 tgctgttacg gttggcgtta aaggaggcat aagcgcggtc gagcgcattt tcccgggtgt
    10681 agcgacgttt ggtcagaaca ttgaaaggca ctgaaacatt aagtccgaca atacgttcgt
    10741 tatcggggat ccctacagat tcattccacg aaaatgagag tgaataactg atgccaaccc
    10801 agctgctggt ataccccacc tgataccagg tatctgaatc cgaagtattc cagtattttt
    10861 gatgggtacc agagatataa agcgagccaa agtcattaag tgattgtgaa atatttaact
    10921 gcaaacggtc tttacggcta aagcgtaaat tgtggtaatt cacgattatc ggttcatccc
    10981 gatgctcacc gtcataatcg taatcatatt cgtacccctc cattcgacga tacgcaacat
    11041 catcaagggt ataaaaacct tgtgtcgaat agcggtaacc cattaactga aaattggtgc
    11101 cgaaggtgtt catcgatttc gcatagagga agcgaatgga atccccctca tgacgactgt
    11161 cgtcggctaa ctgactgcgc gcatgggtca catccagcga cactgcgccc cagttcccga
    11221 ggttgcgccc cagccctaat aaaaaggcgg tgtaattggc agataattgc gtcccgccgt
    11281 aggcagtaaa ttcctgtggt aaaccgccga gtgccgtgcc ctgaaaaaag aaaggcgatg
    11341 attgctgact attaccgcta cgaaaatcgc ccgccgtcag gtcaaatttg aaacgccctt
    11401 cgcgttgtaa aattggcact gttgaatacg gaatagtgta attctgctga ttgccatcgc
    11461 gttcgtcgat ggtgacgtcc agatcgccat ttgaagatgt cgggtgcaaa tctgtaattt
    11521 caaaagcgcc gggggaaaca tagctttgat agataataaa accattttgc cgaatcgtta
    11581 gctgggccgc cgtacgggca atcccacgta ccgttggggc aaacccttgc tggctatcag
    11641 gatacatatt atcggaagaa taaagccgta caccacgaaa tccaacacca tcgaaaatat
    11701 cacttcctgt attgccgtct cccattacca gttcactttt cagcggaata atggcgcgct
    11761 gtacccaggt gccaatatta ttccactgtt ctgaatgata tccatttccg cgaaaatagt
    11821 tccaggaacc attgttgcgt aaacgccacg ggccaatatt aatcccgctg agctcactaa
    11881 aaaaatagct atcgttacca ttacctctgt taccggtgaa attataattc aggagtaaag
    11941 caggaatacc ttcatcccac tcttcaggcg gaatgtaacc gtgagcgcta ctcaacaacg
    12001 ctatctgagg aatagtgatg ttcaggcgca tcgctgcaaa atcaaagtta attgtggcat
    12061 caggtacagc tttcagtaaa ttgatgcatt tattgttttg ctgctgggct aactcgggaa
    12121 atgcactgct attaaggcca attcgttcaa ggagtacctg gttaaaacag ggcattaacc
    12181 cacctgattt atcacctgta ttttctgttg tcgattcaaa tacgatatcc tgtgaaccaa
    12241 tgaactcatc attacgccag agatccaccc gatacacgcc cgcaggttga tgattacctc
    12301 tctcaaagcg cgataaatca gcaacgaatg tgccattctc taataaaaat gccgggttga
    12361 aataactttc cgcatagctt aaaaaggagg gtaaaatggc aagagatgaa taacaaattc
    12421 cggccagaca ccaggtatac ctctgcggaa tatccgtagt agtgggtatt ttcataatta
    12481 gctctgatat tgtcagatga ttcggttagt ttaagttcac ttctctgacc ggggttactg
    12541 aaccataatc attaacggtc tgaaatttca gctttccctg cactcctgag ggtaagggaa
    12601 tttgtgaatt aactctgggt gcagccatag cattccccaa cttttggctg cccacttgta
    12661 agttaaccag ggtgacataa aaaggtgatg gattatgaac attgatatag ttaccggatc
    12721 gcgaaaactt gagtgtatcc ggcgcttctg cgggtaattc ttgtagttga attggacgga
    12781 gaaataactt catgcgcgat aaaatcgcca gttgcaaaac attcctgccg ttcaatgcat
    12841 ttttatctac cgaaggaatc gtcttaacat tcatccagaa cagagactca cgatctgctg
    12901 ccagcggtgg accggtgtaa ataatacgca aagtattttc gcttttgggt tcactaacaa
    12961 atagtggcgg tgtaatgatg aatgactttt cttttacacc gctgctgttt tcaatccacg
    13021 aattgaccag aaaacgctca ttggtatggc tatttctaat ccataccgca gtctgtttag
    13081 catcagcggg ataaataata cgcgtggcac ctaatgcgat ccctccggca gcatggacgc
    13141 tggccgaaat gattaaataa aatataatga
//
LOCUS       DAZFTN010000125        12849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFTN010000125 DAZFTN010000000
VERSION     DAZFTN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1411
                     /gene="zorA2"
                     /locus_tag="ACHKV2_004082"
     CDS             <1..1411
                     /gene="zorA2"
                     /locus_tag="ACHKV2_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFO6941871.1"
                     /translation="PVLLGGLKTAFITSIVGMFFAILFNGMDAFFFANKRSALAENNP
                     ESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQAQLANHTH
                     FSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDFNQNLTEQFGENFKALDASVKKLV
                     EWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEECKEIPLAMSELREVLQVNQHQ
                     ISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKSGAANVSASLEQTSQQILLNA
                     DSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSETLTSTLGETITEMKQSGEEFL
                     KSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSFDKASAGL
                     TSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLGNALLSISKGFVGNYEKLIKD
                     YQIVMGQLQALISANKHRG"
     gene            1418..2125
                     /gene="zorB2"
                     /locus_tag="ACHKV2_004083"
     CDS             1418..2125
                     /gene="zorB2"
                     /locus_tag="ACHKV2_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFO6941872.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            2129..3232
                     /gene="zorE"
                     /locus_tag="ACHKV2_004084"
     CDS             2129..3232
                     /gene="zorE"
                     /locus_tag="ACHKV2_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFO6941873.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            3457..3699
                     /locus_tag="ACHKV2_004085"
                     /pseudo
     CDS             3457..3699
                     /locus_tag="ACHKV2_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            3773..4624
                     /locus_tag="ACHKV2_004086"
     CDS             3773..4624
                     /locus_tag="ACHKV2_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO6941874.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(4822..5009)
                     /locus_tag="ACHKV2_004087"
                     /pseudo
     CDS             complement(4822..5009)
                     /locus_tag="ACHKV2_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5129..5911)
                     /locus_tag="ACHKV2_004088"
                     /pseudo
     CDS             complement(5129..5911)
                     /locus_tag="ACHKV2_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(6627..7085)
                     /locus_tag="ACHKV2_004089"
     CDS             complement(6627..7085)
                     /locus_tag="ACHKV2_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6941875.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            7208..7981
                     /locus_tag="ACHKV2_004090"
     CDS             7208..7981
                     /locus_tag="ACHKV2_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941876.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            8133..9449
                     /locus_tag="ACHKV2_004091"
     CDS             8133..9449
                     /locus_tag="ACHKV2_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941877.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(9648..10217)
                     /locus_tag="ACHKV2_004092"
     CDS             complement(9648..10217)
                     /locus_tag="ACHKV2_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6941878.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            10410..10877
                     /locus_tag="ACHKV2_004093"
     CDS             10410..10877
                     /locus_tag="ACHKV2_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFO6941879.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            11881..12213
                     /locus_tag="ACHKV2_004094"
     CDS             11881..12213
                     /locus_tag="ACHKV2_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFO6941880.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            12335..12712
                     /locus_tag="ACHKV2_004095"
     CDS             12335..12712
                     /locus_tag="ACHKV2_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFO6941881.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
BASE COUNT         3896 a         2767 c         2796 g         3390 t
ORIGIN      
        1 ccccgtatta ttaggtggcc taaaaacagc attcattaca agcattgtag gtatgttttt
       61 tgccatttta tttaatggaa tggatgcttt cttttttgcc aataaacgaa gtgcgttagc
      121 agaaaataac cctgaatctg ttacacctga acatatctat catgaattaa aagagcagaa
      181 ccagactctg actaaattag tctcgggtat taacggtgat agtgaaggtt ctcttattgc
      241 tcaaataaaa ttactacgta ctgagattag cgattcctcg caggcacaat tagctaatca
      301 cactcatttc agtaataagc tttgggaaca acttgaacaa tttgcagatc taatggcaaa
      361 aggtgctaca gaacaaatta ttgatgcttt gcgacaagtc attattgatt ttaatcaaaa
      421 tttaactgaa cagtttggtg aaaactttaa agctcttgat gcctctgtaa aaaaacttgt
      481 tgagtggcag ggaaattata aaacgcaaat tgagcagatg tcagaacaat atcaacaaag
      541 tgtcgagtcc ctggttgaaa caaaaactgc ggttgcaggg atttgggaag aatgtaaaga
      601 aattcctctg gctatgtctg aactgcgtga agtgcttcag gtgaaccaac atcaaatcag
      661 cgaactctcc cgccatttag aaacctttgt cgccatccgc gataaagcta caaccgtatt
      721 acctgaaata cagaacaaaa tggctgaagt gggtgaactg ctgaaatccg gagctgcaaa
      781 tgttagtgca tctcttgagc aaaccagcca gcaaatactt cttaatgcag attcaatgcg
      841 cgttgccctg gatgaaggta ccgaaggatt cagacaatcg gttacccaaa cacaacaagc
      901 atttgcctcg atggcacatg atgtcagcaa ttcctccgaa accctaacca gcacgttagg
      961 tgaaacaatt actgaaatga aacaaagtgg tgaagaattc ctgaaatcac tagagtcgca
     1021 ctcgaaagaa ttgcatagaa atatggaaca aaatacgacg aatgtgattg atatgttcag
     1081 taagactggt gaaaagatta accatcaact atccagtaat gccgataata tgtttgattc
     1141 aatccagaca tcatttgata aggcaagtgc agggctgact tctcaagtca gagaatcaat
     1201 tgaaaaattt gctctatcca tcaacgagca gttacatgct tttgagcaag caactgaacg
     1261 tgaaatgaac cgtgaaatgc aatcattagg taatgctctg ctttcaatca gcaaaggttt
     1321 tgtcggtaac tatgaaaaac ttattaaaga ttaccaaata gttatggggc agttacaagc
     1381 attaatttct gctaataaac atcgcgggta atcgatcatg gataagatta tagggaaaca
     1441 attacctaaa aaagatcaag ataatgaaca ttgggtatcc atgtcggacc taatggctgg
     1501 gctgatgatg gtttttatgt tcatatctat tgcttatatg cactacgtac gtattgaaaa
     1561 agaaaaaatt aaagaagttg ccgtagccta cgagaatgct cagttacagc tttataatgc
     1621 tctggatatt gagtttgcaa aggatttaca agactgggat gcagagatcg ataaacagac
     1681 tctggaggtt cgatttaaat caccggatgt tttatttggc ttaggaagca cagagctaaa
     1741 accaaagttt aaactcattc ttgacgactt ctttcctcgc tacctaaaag ttctagataa
     1801 ttatcaggaa catattactg aagtccgcat tgaaggtcac acaagtactg actggacagg
     1861 aacaacgaat cctgatattg cttattttaa taatatggca ctatcgcaag gtcgtacacg
     1921 tgcagtatta caatacgttt atgacataaa aaatatcgcg acacaccaac aatgggttaa
     1981 aagtaaattt gccgcagtag gttattcatc tgcacatccc attcttgata aaaccggcaa
     2041 ggaggatcct aatcgctctc gtcgtgtcac cttcaaagtt gtaacaaatg ccgagttgca
     2101 gattagaaag attattcagg agtaagagat gaaattatct atcgacattt cagaacttat
     2161 tcaattaggg aagaaaatgt taccagaagg agtcgatttt tttctggatg aatcccctat
     2221 tgactttgat cctatagata ttgagttatc cacgggtaaa gaagttagta tcgaagatct
     2281 tgaccctggt agcgggctta tctcttatca tggccgccag gttcttttat atattcggga
     2341 ccattcaggg cgttatgatg cggctatcgt agatggcgaa aaaggaaaac gttttcatat
     2401 tgcctggtgc agaactcttg atgaaatgcg ccataaaaat cgatttgaaa ggtatcatgc
     2461 aactaaccgc atagatggtt tattcgaaat tgatgatggt tcaggtcgga gccaggatgt
     2521 tgatttacgg gtatgtatga attgcctcga acgacttaat tataaaggaa gtattgataa
     2581 acaacgaaaa agagagattt ttaaatcatt ctcattaaat gagttttttt cagattatag
     2641 tacttgtttt cgtcatatgc ctaagggtat ctatgacaaa acaaatagtg ggtatgtcga
     2701 aaactggaag gaaatatcta aagaaatacg agaaaaggca aattatgttt gtaatgattg
     2761 tggcgtgaat ttatcaaccg ccaaaaactt gtgccatgtc catcataaaa atggcatcaa
     2821 atatgataat caccatgaaa accttcttgt tctgtgcaag gattgccatc gaaaacagcc
     2881 cctccacgaa ggtatattcg ttacccaagc agagatggct atcattcaac gtttacgttc
     2941 ccaacaaggg ttattaaaag cagaatcctg gaatgaaata tatgacctga ctgatccatc
     3001 agtgcatggt gatattaata tgatgcaaca taaaggcttt caacctcctg ttcctgggtt
     3061 agatcttcca aactcagaac atgaaattat tgcaaccgta gaagctgcat ggccaggcct
     3121 taaaattgca gttaacctta ctcccgccga agtcgaagga tggagaatat ataccgtggg
     3181 tgagctggtt aaagaaatac aaacaggagc ctttacgcca gcaaaattgt aaattctaaa
     3241 actccgtgaa agttaagact ttcacggaag ataaataaaa tttccctgat ttgtggctca
     3301 aattacaaaa gtagtttatg gcataacttg tctgattttt atggtgtaac aggtataaaa
     3361 gcatatgcta tggttcgcct catacttaaa acttccctca tatgggtgaa ggttaaagct
     3421 tggtagacag aagacagtca caatgaataa agcaataaat tgatgtcgca cgcgccgatg
     3481 ggcatggcgg gtaatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt
     3541 aaccagaaca tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcagca
     3601 gctatagaca agatcaatgc ccattatttc gacaaaaaag cagaagatta tttcttcaat
     3661 aagtttttgt tgtcttttga tccctcaaca cagcaatgga tcttcacttg caggcttgaa
     3721 cagcagaaaa tttgcgagca tgattgcaat tatttcactg cctgggatat caatggcttc
     3781 cagtaaatcg ctacagcagg caattgccaa cataaaaatc tggcacaagg gtgaacagcg
     3841 cgcgccgcat aaaccattgt tactgctata cgtattagcg ggatacctga atggacatcc
     3901 gcgccttttc gattatggca cggaaatcta cgaacctctg cacagtttac tggaacgttt
     3961 tgggccacag cgttcacagt accgacctga tatgccgttc tggcgattac aaggcgacgg
     4021 aatctggcaa ttacacaatg ccgagcgttg ctcaacagca gggagcagcc gacaaccgcc
     4081 agtgaaggaa ctgaacgaat accacgttgc aggcggtttc gatgaacagc actacgccct
     4141 ggtaaccggc aataaaaaac tcatcaatac tctggcgcaa cagatactcg aagcgcactt
     4201 caccgagagt attcaggaag aaatcgctga cgagctgggt tttgatctcc agcaaacccg
     4261 taaacagcgc gacccgttat ttcgcaaaaa tgtattacgc gcttataact accagtgcgc
     4321 aatatgtggc tttaatatgc gccacgatga caccaccgtc gcgctggaag cggcgcatat
     4381 aaaatggaaa cagcacggcg gcccttgtga aatccccaat ggtctggcgc tttgcgcaat
     4441 acaccataaa gcctttgata aaggatcgat tggcctggat gaaaatatgc gggtactggt
     4501 ctcggatgcg gtaaacggcg gagggatcgt tgagagatta ttctgggatt ttgacgggaa
     4561 aactattgcg ttgccgcagg tgcgtaaaaa ttacccttat gaggggtttg tgggtgagag
     4621 atagacattc tgtttactaa taatttatag ccattctctg ctcctgcatc agagatatga
     4681 tgcaggaata ttaaatacca gccacctata aattatgatt tattaatttc tgcttcagtt
     4741 aaatccctct gaaaatagac ccgttttagt tccatacatc tttaaaaatc ccgaccaaat
     4801 aatgagatca gcaaacatct atttgaaacg ttggttctca tcctcaagat ccttgattat
     4861 tttaatatca gaaaattcca taccgccgta tctggacttg cagttgtagt aagtggcttc
     4921 agataagcca catctttaac agttcgcccg tattcaagcg atttaatcac ggcgataatc
     4981 tgatgctcag taaaaggggc tttacgaata gcgatctcct tcattggcag attgattatg
     5041 ccggaggatc tctaaatgtg aatggcacga ttatgcggga tatttactgt accagtccaa
     5101 ccctctgctt actgcaatac caatcaagag ggatggtcca cattgacgtt tcaaaatcga
     5161 tttctgacca gcgagcaaaa tgcagcttac tggaccgaat aaagataagc agagtgagtt
     5221 ctgtcgtaca gtgggttagc ggtctgccgg tgtagctgtc tattttgtca agcaactcag
     5281 ggatacgctt taactccagc gccggacgat gctgtcgatt actggaagca accgcccccg
     5341 acatctcttg cgccggatcg taatcaatta acccgctttg cactgcataa cgcatgatgg
     5401 ccgtggtgcg ctgttgaagg cgggaagcca cttcaagacg cccggatttc tccacggctt
     5461 taataggaat taacagatca cgggtgccta gttcagcaat gttgcgagta ccaattgtcg
     5521 ggaagatatt gtcctccagg ctctttttta cacgattagc atgatcttcc gaccattttt
     5581 ggttggttac gagccactct ctggcaacct tctcgaaagt aataatctct ttcgcctgct
     5641 cttctttcac agcacgctta tgctcacgag gatcgatacc agcagctacc agctttctgg
     5701 cctcttccct tttcttccgc gcatccgcca gtgaaacatc cgggtaaacg ccgaacgcca
     5761 tcaaatgttg tttaccgcca aaacggaaac gaaatcgcca atactttgaa ccgccaggtt
     5821 ttacaagtaa ggacatgccg tcgccgtcaa caagtgaata ctctttctcc tcaggtttcg
     5881 cagagcgaac tttagtatcc gttagggcca taatggtctt ccttccattg gtatctcgtg
     5941 agtatacaag caatatcgaa tcggcaaata tactcattag tatactcaca agcctgttga
     6001 tgtgggttgg ttcaggttga cttcagttga ggagaaaagc tggaaaagcc ttgcgtggcg
     6061 cggatttcag gcataaaaaa agacctcagt tgaggtctat ttacatactt ttggtgccga
     6121 aggccggact cgaacctaag tctccataac ctgctgtaat cacgttccta ttagccttta
     6181 aaagaacaat cgtaggacct ttttgttcta ggggctacat cttaagtttg atccgctgga
     6241 cggaatcaaa ccgagtgttc gtggcggcac aaatcattaa cgtgccatac ctgccttttg
     6301 atgtttatgg aggtctagtg ggtcgggagg aaagaacatt tgtcagctat gtggatgtta
     6361 agtaaacgcc actgcgttcg gattttgaaa ccagttaaac cccgtcagac cttgttcaga
     6421 ataactattt aactcaatgt tatttatatg aattaagcca ttcagcgtca aagtaaaacg
     6481 ccaccgtaac gcctacgtaa cgcacccgca ttttgctgtt ttattcactc ttatagaaac
     6541 aaagagctga tgaaacgtga ggctatcagc atctaatgaa cactgttcat ccatacagca
     6601 tgaaaattca taaacttaca cttgagctac tttctcagct tgtcccaccg aaccgcagat
     6661 acaatacccc agttgccgct aggattaggc ctacgccatt ttgctgtctc catgctacga
     6721 agatactcaa gagcttcaga aaacgtgcca aaccgcttct gagccgtttt atcacctacg
     6781 ccaaacgagc catcgcgagt gcttgccagc ccgggatgaa aacacgagcc atcagctgca
     6841 taaggaacca gtaattccgg gtcactatct tcagatgaat cagacaccaa tagtcgccac
     6901 ggctcaacac tcagagcatt agccagcacc tcaatcgcat ccaaagacgc attagagcca
     6961 ccgcgttcaa tgcggctcat atagctacga gcaaaaccac accgatcggc aaaggcttct
     7021 tggctcattc cggtggcaat gcgcaactct tttactcgtt gtccaaacag ttttctcagg
     7081 gtcattttca tgacctgaat ttgcgcagat gtccactaaa gagccacgct ccaatagtga
     7141 cattttgaat caatggtcta tgatgcgata actgacaccg ctggggtgga ttctgaggga
     7201 taacaacatg tcaaaaacac ggcttagtaa gcccggtttt tcagccaagt ctgtgctgac
     7261 cattatgtta atcgctgcgt catctgcatc ttatgcagca gaaagcatcg atcaactggc
     7321 gacgcgcatc ggtatggatc acttccggga agcgatcaaa aagggtgaac gcagcgcctt
     7381 caaaacgctg ccggcaaaag gctataaaaa cggtcttacc gggctgttaa tcaaaactaa
     7441 cacttacgtt aaccccgaat acagctttgc tgattgcgat acggacaccg ttattatctc
     7501 ggtgcaagaa cacaaaacgc ttcccggttg ccgtatatct ttaaagctag cgacaaaaga
     7561 cgcagatggc agttatcacg acaatggaat tcgcgttgaa tacggtattg ccatcggctc
     7621 caaaaagttt atcgctaaca attcagcgtg ggcgcaacat gccatcagcg gtgataaagt
     7681 gttggaaggc gctctccacc cggataaaac gaataacaat aaagatcaaa tgaataacaa
     7741 taaagatcaa atgtgcatgg gtatggctca atttaccgca aacgtaaccg aaggccgtaa
     7801 cagtgggatg acgaaaagag aatattatca gcgacttgat aacatgaacg gtaaggccga
     7861 cgtcacccag cagatgataa cgctctataa gacttttgtt gactacgtat atgaacatcc
     7921 aaagcaagac gcaaagattt cagggacgta tatttatact aactgtatga acaacttcta
     7981 gaaacgtagg tgcctgaaca tgatagaact cagctgccag catagagtgg aaagcggagg
     8041 cttaccatcg tatattcatc gcgttatctt tccgtaacta taatctgtct cagttgatct
     8101 agtgcctttc agggctgaac taaggaataa agatgaatga cggtgttatg ctagattctg
     8161 acgttattgc tggtcttgag tggttagcat caactcaaga gaacaaacaa cacttttacc
     8221 agcgcttagc aaaagcacag caattttaca tagctacaac ccaaaaagct gccaatttcg
     8281 ggaaacagtt tgacccagaa tggtatggtt ccgacgtggt tgctggttat ttcgcacaag
     8341 caaaatcgct tatcgataat agaaggtctt acgaagttac taatgcttca aatataatac
     8401 catgggttaa acaattagga gtgtgtgcta aatcgctcga tagaattact ggcgctagag
     8461 atcgcgcaat cagaatgctt aagaatacaa cagtattacc agacacagcc ttattagagt
     8521 tggttcttgc tggaaactat gcatcagaag gttatgaagt tgagttcatt ccagaacaaa
     8581 aagggatagc aaaaaccccg gagtttcgtt gccgccttgg agatggcgat tatttctttg
     8641 tcgaatgcaa aaggcttcag aaaggtcctt acgtaaagca ggaaatatta cagcatcata
     8701 tacgctcaca tcttgccgaa cttcatatca aatccaaaaa gatgaacgta tggactgatg
     8761 tcacatattt atgtgaagtt aaagacgcgc ctgaaaacta tatcattagc cacttaaaaa
     8821 actttaaagg tgtattttat gaatggaatg atgattatgg aaaaggaacg attagaccgg
     8881 ccaacttaaa tcttatcagg gaagatatca ctgaaaacgg ctcattacta gtcaacacta
     8941 aacttgctcg tcttatcaaa ggctcccctc tggaagatga aaattaccaa gttgttgcta
     9001 tgggacgacc agatgagcgc gactcacgtt tcataaccaa agttaaactt gcttctttga
     9061 taacatggcg ttgtatcaat gaaaaatctt tcgatgcacg ttcgcgacat gtaacaaaaa
     9121 cattagcaga gatagataac cagcttttgg attatggtct aggcgtaggg catatagctt
     9181 tggatgtaga tattcaaaag gatgttgccg ataaacgaag ggaaaaaaac tatgctgcca
     9241 tagtaaattt tgagcaaaaa tctaaaatag tccgcctgaa tgttcattat ctcgtgccac
     9301 gcatagatga gaactctgcg tggatggtag atgaaactgc ggatgacttt tttacccatg
     9361 atctggtcaa atatgttatc ccattgacta aaatattccc cgaggctgag gtatttgaga
     9421 atgaccaacc tggctggcat caaaattaat ttatttttga tagtatgaac gagggtagca
     9481 tttctataca aatcagtgtt cggctgatag cgagttaaac agtttactga aatgttctca
     9541 gcaattagct gttgccagga cctcaaaaat ccgattctgg cggaaagtgt gctgttttac
     9601 caagtattgc tcgtatctgc aaagtgttag agcatgtttt aagtatatca gtaaccctta
     9661 ttcagtaatg cagcaagcgt cgctcttgaa acaccgtatt cacgcgccaa tgcgctaaca
     9721 ctaaccccag cggccctttt gtctaaaatc gctccacgag cttccggtgc gattttagca
     9781 ggcctgccca atttcctgcc ttcagctttt gcccgtaaca gccccgcatt agtccgttca
     9841 atcagcaggt cacgctccat ctccgcaacc gctgctagca tcgacaacag gagcttaccg
     9901 gcagcagagg tgagatcagt ggtgccaagc tgatggacaa taaccttgat gtttcggtca
     9961 gccaacgtcc tgacagtttg cagaacgtca attgcatcac gcccgaggcg gtccagcttt
    10021 gcaactacca acgtttcccc atcgcggatt ttgctgagca tttcaaaaaa cgctttgcgt
    10081 tgcaccgcag gaacctttcc gctgactaca tcagaaaacc aaaagtcgaa cgtccaccca
    10141 gcttgttcaa gttccatgcg ctggttctca gtatcctgct gggatgtcga aacacgcccg
    10201 tagccaaaaa tagccatagt catctcctca tccggcccct gccagaaaac ggtgatgaaa
    10261 ataggatggc ctgccggaaa agccaaggct aattttccaa cagtgtgaaa gcgtgcggtt
    10321 ggaggatgcc ggaaaacggt cgtttgttgg cgcttaaaac gggtgaagac atgttaggct
    10381 gaaaccatca cttcataagg acaaccgaaa tgattaaaga cgcgattttt tcaccgtgcg
    10441 gtaagtatcg ctactcgtta tcacgagtct gggacgaatc aaaaccgtat acccttttca
    10501 tagggctaaa cccttcatac gccgatgccg agaaggatga tcgcaccttg agccgctgca
    10561 tatcgtttgc caaaagctgg ggctacggtg gcgtatacat ggccaacctg ttcgcattcg
    10621 ttcacaccca gcgacatgaa atgatgaaag cgtctgatcc cattggcaaa gataacgata
    10681 gccacttgat acgtcttgtt agcggtgccg gactggttgt cgccgcatgg ggaaatgaag
    10741 gaagacacct caaacgctca acgaccgtcc gacagctatt gccagagagc acaatgtgct
    10801 tcgtcttaaa cgctacaggt gagccgaagc accctttgta tatgaaaaac gacagtgttc
    10861 tcataccttt aggttaacat cgataactgc gttcagaata tgaacgcagc catatgatat
    10921 atatgacttt tctttaaaat gatggtgtgc tagcttctct cctgacacct acctcatgcg
    10981 gtaaaccggt ccgggtaggt ggagatcacc gctgtttagc ggaagacgtc aggatgggaa
    11041 ctctgcgtaa cgttgattgt ggtaggtagg gcgcgcaagc gtttgatgca gccaagcaga
    11101 gggtgaaagt agctatcggc ggattcttaa gtagcttctt ttgcgcataa tgcatctttc
    11161 aagaaaggtc gtcacaccac gaggaagact tgatcttcat ctggcaaggg gaaccgagca
    11221 aacctacagg ctatcttctc agatggcctt agggttgata tgtccggctt cccccccgag
    11281 gaagggttta catcttaaag aacaagttat gttttaacat ttttaacaat tacacttctc
    11341 tgctgtttgg tgtcttatgg cgttcccgtc aaaagtaaag ctgccgtttt tacctcacat
    11401 gcgtaacaac caaacacgaa ccagaagctg ctaacgttca acgtgttcgc ggtgtaaatg
    11461 cagccggata agttgggagc gaaaaagtgg atgtcctagg tttggaagcg cccctcttaa
    11521 gcattgcatc cgataatttc cgtggcggtg agacagtcct tatagaggca atcagcctcc
    11581 aaagttaagc gacgcggtaa aactaaagct cgcccagatt tctcattgat tgctcgaact
    11641 ccgctacgct tgtttcaact ggcttcacca gtcactctat gagagcgccg cgaattctgc
    11701 accatgattt cgactacctt ctttaaaaca ctcgccagag agccttacag cgcgtttgaa
    11761 ccgcatatcg cagcttctgc ttacggatga ctgtctgatt agcagtagct tcatggccca
    11821 gtccggttta ctttattgct atcaactagg agccgcccaa gggcaacacg gagataacgc
    11881 ttgagaaccg tcgaaatact gcaatacacc ttacgcaaag gcagcggtgc cgcttttcac
    11941 gccataatgc aggaaatcag tgttccccta caccaaagcc acgggatcga tgttgtttcg
    12001 tttggcaact cgcttgatga cctggattgc tactacttga ttcgcgcctt tgacagcgct
    12061 aaaagcatga ctgcggttct cgatgctttt tacgcaagcg cagactggcg cagcggccca
    12121 cgcgaagaca tcattggcag catagagaac tgcattaaaa cagtgattag cctaccatca
    12181 gaaagcgtga aagggctgcg gatgcaatcg taagtaatca agtgtattag cttagatttg
    12241 acctaaacag ctttgtgcca aaagtggact aggaaaagcc tagcgataaa gagttgagtt
    12301 taataactac taactgaaat ttaatgggtg atatatgaat ttagatgaag aattttacga
    12361 attacaaaaa ctctttgctc agaaggatct actgactgag aaaagacgct ctagtggcgg
    12421 cggattcatg gaaattctct tggtgaagag acagaacatg aagataaaaa tatatcaaga
    12481 aaaaggacat caacttcccc atattcatat tgactatgga aaacaagcgc atgctgccag
    12541 ttatgccata caatcaggag accgcataga gggagattta cctaagaaat atgatagtga
    12601 tgtttcgagt tggttgggga gaaatcgtga taaaatttta gaaatctgga atgcactaca
    12661 agctggcgca ccttatgaac cactaatcgc agaattgact ggcggagtgt gactaactaa
    12721 ataattttcc cctaaaaaaa ttcgatgtag gaaaatatat ataagtaaga atcatgagtg
    12781 tccgctcctc gctcaaggcg gactgttaga cttgattgta tgctgtcaga gaaaaccgtc
    12841 agatcaatt
//
LOCUS       DAZFTN010000126        12652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFTN010000126 DAZFTN010000000
VERSION     DAZFTN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..1132
                     /gene="phoE"
                     /locus_tag="ACHKV2_004096"
     CDS             77..1132
                     /gene="phoE"
                     /locus_tag="ACHKV2_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6941882.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHKV2_004097"
     CDS             complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHKV2_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6941883.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHKV2_004098"
     CDS             complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHKV2_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6941884.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHKV2_004099"
     CDS             complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHKV2_004099"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6941885.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3685..5142
                     /gene="pepD"
                     /locus_tag="ACHKV2_004100"
     CDS             3685..5142
                     /gene="pepD"
                     /locus_tag="ACHKV2_004100"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6941886.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5199..5720)
                     /gene="prfH"
                     /locus_tag="ACHKV2_004101"
                     /pseudo
     CDS             complement(5199..5720)
                     /gene="prfH"
                     /locus_tag="ACHKV2_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5719..5922)
                     /locus_tag="ACHKV2_004102"
                     /pseudo
     CDS             complement(5719..5922)
                     /locus_tag="ACHKV2_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6241..6693)
                     /gene="yafP"
                     /locus_tag="ACHKV2_004103"
     CDS             complement(6241..6693)
                     /gene="yafP"
                     /locus_tag="ACHKV2_004103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6941887.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(6703..7101)
                     /gene="yafO"
                     /locus_tag="ACHKV2_004104"
     CDS             complement(6703..7101)
                     /gene="yafO"
                     /locus_tag="ACHKV2_004104"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO6941888.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(7104..7397)
                     /gene="yafN"
                     /locus_tag="ACHKV2_004105"
     CDS             complement(7104..7397)
                     /gene="yafN"
                     /locus_tag="ACHKV2_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO6941889.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(7449..8504)
                     /gene="dinB"
                     /locus_tag="ACHKV2_004106"
     CDS             complement(7449..8504)
                     /gene="dinB"
                     /locus_tag="ACHKV2_004106"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6941890.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8575..9360)
                     /gene="lafU"
                     /locus_tag="ACHKV2_004107"
     CDS             complement(8575..9360)
                     /gene="lafU"
                     /locus_tag="ACHKV2_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6941891.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9332..11044
                     /locus_tag="ACHKV2_004108"
                     /pseudo
     CDS             9332..11044
                     /locus_tag="ACHKV2_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(11189..11686)
                     /gene="rayT"
                     /locus_tag="ACHKV2_004109"
                     /pseudo
     CDS             complement(11189..11686)
                     /gene="rayT"
                     /locus_tag="ACHKV2_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(11862..12581)
                     /gene="yafL"
                     /locus_tag="ACHKV2_004110"
     CDS             complement(11862..12581)
                     /gene="yafL"
                     /locus_tag="ACHKV2_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6941892.1"
                     /translation="MSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQT
                     HLKKLVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSS
                     DHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEM
                     YHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLA
                     EPFWQDHFLGARRILTEETIL"
BASE COUNT         3130 a         3241 c         3198 g         3083 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc agaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg
      301 tcgttgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac agcaaaaaac
      361 gcgtctcgct tttgccggat tgaagtataa agatttgggt tctttcgact atggccgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcttggg ctacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggggctacat attatttcaa
     1021 caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt
     1081 gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatgattatt
     1141 gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg gctcgtcacg
     1201 aaaatcatcc gccggttcca acttcagatt cagcgtcgtc agcagttcac gcagcttctc
     1261 gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtatctt ttaccggaac
     1321 actcttccag tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga
     1381 ttcctgcgct tcaagctcca tccaccagcc ccagaactca cgcacttccg gtgccggttt
     1441 cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc
     1501 acgaatatac gggcctagta cggtaaattt tttgatcaat ctgctcttcg ggtgtccact
     1561 cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaaattt
     1621 agcaaatttt taacacaagc gtttttcgat ccagtcggtg atttcctgaa gacctttgtc
     1681 aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga
     1741 ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa
     1801 cattggcgtt gggcatcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag
     1861 ctccacgcgc aacgcttcgt cggaagcatc atgcatcccc aaacgactcg ccagaacgtc
     1921 aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac
     1981 cggaccaaga caggcaaccg ctttcagacg cggcgattca agatatgcca gacgcacggc
     2041 aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac
     2101 gttaggcaga gcctttaaga cgcgctgatg caacaggcag gagtcctggg tgagcttcca
     2161 ttttgaagaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc
     2221 aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat
     2281 taataccgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc
     2341 accgccgggt acggtaaact ccatctgtcg catcgtgccc ggtagacgct gagcggcctc
     2401 ttcataggcg cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg
     2461 aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgacgac cactgagttg
     2521 cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata
     2581 aatccagttg ccgccacggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc
     2641 ggcatcgctc atcacaatac gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg
     2701 ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag
     2761 ggccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc
     2821 aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc
     2881 agcctccaga atacttactg gaaactattg taactcgcct gaagttaaaa agaacaacgc
     2941 ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc
     3001 ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca taatcatcaa
     3061 ccagcggacg accagccggt tttgcaaaga tggtgacaaa gtgcgctttt ggatacattt
     3121 cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt
     3181 cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc
     3241 tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg
     3301 gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc
     3361 ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc
     3421 tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag
     3481 gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt
     3541 ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc
     3601 aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc
     3661 gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg
     3721 tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc
     3781 gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta
     3841 ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc
     3901 ttgcaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg
     3961 aaagatccta tccagccata tattgatggc gaatgggtta aagcgcgcgg caccacgctg
     4021 ggtgcggata acggcattgg tatggcctct gcactggcgg ttctggctga cgaaaacgtg
     4081 gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg
     4141 ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa
     4201 gaaggtgaaa tctacatggg ttgcgcgggg ggtatcgact tcacctccaa cctgcattta
     4261 gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa
     4321 ggcggtcact ccggcgggga aatccacgtt ggcctgggta atgccaacaa actgctggtg
     4381 cgcttcctgg cgggtcatgc agaagaactg gacctgcgtc ttattgattt caacggcggc
     4441 acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa
     4501 gtcgacgccc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca
     4561 gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg
     4621 attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc
     4681 cgcaactccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc
     4741 atgactgaca ataacgtaga aattcactgt ctgatccgtt ctctgatcga cagcggtaaa
     4801 gactacgtgg tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg
     4861 aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt
     4921 gaaacctatc agcgcctgtt caacaagacg ccgaacatcc agattatcca cgcgggcctg
     4981 gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaaca
     5041 atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg
     5101 acactgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg
     5161 gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc
     5221 cctgtgaacg ttcgtcgcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc
     5281 gatttcagca ctgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat
     5341 cgcgccagcc gtttgttagc atgctggctg cgttctgact gaaccttcac gctaatcccg
     5401 gttgccagat gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc
     5461 cccgacggac gcagcgtctc ataacggatt gcatccgatt gttcctgctc atcagcggta
     5521 aaacgcccaa tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcaga
     5581 caaatccact gaatagtgcc gcaccaactt tcgcttaacg cccatgcgtt gtcgccatcc
     5641 agagaaatca gcgccgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccagcacc
     5701 gttaccgcga cgtcttgtcg ggtaaaaacg accaacagag aagaacgctg cgccaatcgg
     5761 gtagccgcgt ccggggtgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt
     5821 tgccgtggtg tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat
     5881 ggtaaatacc gcatcggata agggacgaat atatttgccc atgtaccatt ccatttatac
     5941 tcgtcatact tcaagttgca tgtactgcga ctgcgttcgt tcaccccagt cacttactta
     6001 tgtaagctcc tggggattca ctcgcttgtc gccttcctgc aactcgaatt atttagagta
     6061 tataaatata aaaatcagga aatggcagac gaatccaggg caagaaaaaa aagaattaat
     6121 cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac ctccttgcag
     6181 tttgtggaat aagaaaacgc gcacatacca ggcgggcgtt attttcattg caagctggat
     6241 ttaatgttgc ggtttatatc gcatagaaaa attagtaaac cacgctcccc ggcattcaac
     6301 gcgctgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg cggttatgct
     6361 ggcgtccacc gtaagttcgg attcagactt aatccaaggt tttaacaaag cgctggcaac
     6421 cccacggcgg gtgtattcag ggtcaacaaa taacatatcg atataatgtt caatgcggga
     6481 aataaaacca accggttgtg cattaatgac tgcaacccgc acttgtgatt tcgcgagttt
     6541 ctccttccag cgagattcgt caatctgcgc ccaggcggca atttgttgtg gtgaataatg
     6601 ctgactggcg gtcatcataa ccgctctaat gaaaatagcg catagttgct gaaaatcgcc
     6661 aggctgatag tttcttattt gtatgttatt cataatataa attcaaaaac gcatgcgaaa
     6721 cgcttctgcc attttcccaa ttttatgcat ttggttgtta tctcgagcca gtttatgagg
     6781 ttcaggtttc agaatggcaa tgagcaacca tgcttgctca tcaaacgcac cctgacaata
     6841 taccaaatgc gattcgtcat tcgttctgct gaattggcgc aactgtggcg gaaatggatt
     6901 attcacattt gccagatgaa tatgagcaac tcgctcaaat ttgattaatg gccaagtaaa
     6961 cgagtcgtcg tagagtgcat cgcgaccaaa tatatctggc aaaacaccgt cacgcttata
     7021 ggaaataaaa tccgccgtta acgcatcaag ctcctctgct gtaagttgca ggcgaataag
     7081 ttttgttttg aatacccgca tccttattcc ttaaagtcat tgaaatcatc atccgtcata
     7141 tcattaagat atttttcagc tcggcgtgta atttcctcta atcttgcagc acttggacgg
     7201 aagcgcgcct ttatgggctt tttctcctct tgttcagcaa gcatgtccat taacgcttcg
     7261 aatgcgctgg cacttaagag atatcctgcg gggcgattat tagaaagaac cgcaaccggt
     7321 tgatcaataa agtatttagc tgggttttta cgtaattcag tgatattgac cgatttttca
     7381 gcgagaattc gatgcataca gtgatcccct cataataatg cacaccagaa tatacataat
     7441 agtatacatc ataatcccag caccagttgt ctttccattt gagggtcaag caacgtcaca
     7501 tgcagcccca ccagtcgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg
     7561 gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgggt ggtttgctga
     7621 aaatcgtcga actttaattt caccccctgg cgagcaatca gtaaatcagg ttttaccttt
     7681 gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag
     7741 tgatgaatat cttccgccat cgtgcgttcc acgccgacgg attttcgtaa ccgttcgctg
     7801 ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca
     7861 aagcgtttaa gcagaatcac cagatcacac ttttgtacat caccgcaggt ccgcagcccc
     7921 atcgcttcca gctttgccgc tgagactttg ccaacaccgg gaatttttgc cagcggtaag
     7981 gtttgcagaa atgccgaaac ttctgccggc gtaatcacaa actggccgtt gggtttattc
     8041 atgtcggagg cgattttggc gagaaacttt actggtgcca cgcccgcaga cgccgtcagt
     8101 tgcagctcgc tgaaaattgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg
     8161 caatggacgc tatcagtgac atcgaggtag gcttcatcca gtgacaacgg ttcaatacgc
     8221 gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag
     8281 cgccccggaa gcaaggtgag atgtgggcat aatttgagcg ccatccctgt cggcatagcg
     8341 ctgcgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg
     8401 cggctgccgc caatagcaat agggatatcg cgcagggcgg gattgtcgcg catctccact
     8461 gcggcgaaaa agcagtccat atccacatga atgattttac gcattactca cctctcaaca
     8521 ctggtaaagt atacagtgat ttcagggttt gagaaatgcg taaagattca gcatttagcg
     8581 cgttcgctgc gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt
     8641 atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat
     8701 aatctcaatg cgtcggttgc ccgcgctttg cggatttttc gcgtccagca gcatctggtc
     8761 cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg
     8821 acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta
     8881 ggccatcgca tcggtatgcc cggtaataat aattttgtta tcgagcgagt cgaacactgg
     8941 cgcgagctcc accagcaaag ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa
     9001 catattgcga ttctggtcgt ctttaatcag cacgcgtaat ccctgcggga caatctccat
     9061 ctccagattc gcttccatat gcgcatcacg ggcaatggtg ttgatactgg ttgccagctc
     9121 gccaagctcg gtggcggatt tcttcttcag cagcgccgtt ttctcattca catcacgcgc
     9181 ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct
     9241 gattttattt aacggcgaca gcccaccacc gttaaaaatc gactgcccgt gcagcgcggc
     9301 gataatgctt tcacgctcgg atttactgac gctgttgaca atcaacttta tcgtcgtcac
     9361 caaaggggcc gagcgtattt ccgaggtttc tgcccgcttt accctggatg cgatgcccgg
     9421 caaacagatg gcgattgacg ccgatcttaa cgccggattg atcaaccagg cacaggcgca
     9481 aacccggcgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg acggggcatc
     9541 gaagtttgtg cgcggggatg ccatcgccgg gatgatgatt ctggcgatta acctgatcgg
     9601 cggcgtctgt atcgggatat ttaaatacaa cctgagcgcc gacgccgcct tccagcaata
     9661 tgtgctgatg accatcggcg atgggctggt ggcgcagatc ccttccctcc tgctctccac
     9721 cgcagcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
     9781 ccaccagctg ctggcaagcc cgtcggtgct ctacaccgcc accgggatta tgtttgtgct
     9841 ggcggtggtg ccgggaatgc cgcacctgcc gtttttgctg ttcagcgccc tgctgggttt
     9901 taccggctgg cggatgagca aacagccgca ggcggcggag gcggaagaga aaagcctcga
     9961 aacgctgacc cgcaccatca ctgaaaccag cgagcaacag gtcagttggg aaaccattcc
    10021 gcttatcgag cctatcagtt taagcctcgg ctacaagctg gtggcgctgg tcgataaagc
    10081 gcagggcaac ccgctcaccc agcgtattcg cggcgtacgg caggtgatat ccgacggtaa
    10141 cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgcctcaagc ccagccagta
    10201 cgccattttc atcaacggca ttaaggccga tgaagcggat attccggcgg ataaactgat
    10261 ggcgctgccc tccagcgaaa cctacggcga gattgacggc gtgctgggga acgacccggc
    10321 gtacgggatg ccggtgacct ggatccagcc cgcgcagaag gcgaaggcgc tgaatatggg
    10381 gtatcaggtg atcgacagcg ccagcgtgat tgccacgcat gtgaacaaga ttgtgcgcag
    10441 ctatattcct gatttgttta attatgatga catcacgcag ctgcataacc gcctgtcgtc
    10501 gatggccccg cgcctggcgg aagatttgag cgcggcgctg aattacagcc agttgctgaa
    10561 agtgtaccgt gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
    10621 cgtgctggtc gccagtagcg cggtgactaa agatcatatt ctgctggcgg ccgatgtgcg
    10681 cctggcgctg cggcgcagca ttacccatcc gttcgttcgc aagcaggagc tgacggtgta
    10741 tacgctgaat aatgagctgg aaaatctgct gactaacgta gtgaatcagg cgcaacaggg
    10801 cggtaaagtg atgctcgaca gcgtgccagt ggacccgaat atgctcaacc agttccagag
    10861 cacgatgccg caggtgaaag agcagatgaa agcggcgggg aaagacccgg tgctgctggt
    10921 gccgccgcag ctgcgccctt tgctggcgcg ctatgcaagg ttgtttgcgc cggggctgca
    10981 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagataatgg gggcgttgag
    11041 ttgattggcc tttaaggggc gcatccggca ttggtgcaca attgccggat gcggcgtgaa
    11101 cgccttatcc ggcctacggt tgagtgcagt tttgtaggca tgataagacg cgccagcgtc
    11161 gcatcaggca tccggcacca gcgcacaatt atgaaatgat acgctccccg gcactaaaat
    11221 ccgttatgtc tcccgcccaa tcaatgggat ataaccctcg cgccacatcg cgatggaacg
    11281 ttgagaatgg ccaatcactc acttgcttta cccagccatg ttttactgga tttatataaa
    11341 tataatcaac atgatggcga tagtctttgg tattgcggat ggtgtgctcc taaaaacgtg
    11401 gttgccagat atttttcaat ccacaagcat gggtgaactg ctttttaatc tcccgccagc
    11461 gggaggaaaa atcatcatcg ctttcgggta atgtccatat gcaatgcata tgctccggca
    11521 aaacgaccca ggcgttaatt tcaaaaggtc tgtctcgctt aacgttaata atggcgttac
    11581 ggagcatctg gaactgagcg gtcagaaggt ggcttcgacg atttcgtagg ttaaccgtga
    11641 aaaaccaggt tccccctttg atgtaatacc gacgatattc agacatggca actccttttt
    11701 ctcaacggta agaagaaaag gaaaggtcgg caatgaggtg atggaataac gggaaagcgg
    11761 ctcgcaaaaa cgcacagatt tgtaggcatg ataagacgcg tcagcgtcgt atcaggcatc
    11821 tgcgcacaac tgccggatgc ggcgtgaatg ccttatccgt cctacaaaat cgtctcttcc
    11881 gtcaaaatcc ggcgcgcgcc taaaaagtga tcctgccaga aaggttcagc taaccgactt
    11941 acccgaatgg tttccccggt gcgcggtgac tcaataaact gaccgtcgcc cagatacacc
    12001 cccatatggt cggcaatctc gcggctgtgg atatggaaaa acagcaaatc tccccggcgc
    12061 aggtcgttat tcgccacaat cgttgcccga tggtagtgat acatctcatt agccgtacgc
    12121 gggagcttcg cctcgaggat cttgttgtag gcataaaaaa ccaacccgct acagtcaaag
    12181 cctttatcag gccgcgtgcc gccccagacg taaggcttgc caagctgctg ctccaggcgg
    12241 tgaatcgcca cttcggtaat gttatgcaga tggtcgctgc ttaaaaagtg gttgttttcc
    12301 gccagcgcct gccagcgtct gtcactcgcc ttgagcggtg caggaaacca ttctggatgc
    12361 tgttttatct gttcgcggtt gtgctggcgt agcgcccttc tgctttcggc attgccttcc
    12421 acaatatagc tgaccagctt tttcagatgg gtttggtact gttttaataa acgcgcacgg
    12481 ttttgcatcc gctggcgggc ggcgtagctg aaagagatat gtgatgtggt ggcgctggca
    12541 aatgaagaaa acggcaaaca aatcaacagt aatcccgaca atacaaagga tggaatcgac
    12601 ggcaaggaca tactcaataa tggctggtca tgcattgatg ttacagtgta tc
//
LOCUS       DAZFTN010000127        12504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFTN010000127 DAZFTN010000000
VERSION     DAZFTN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2881)
                     /locus_tag="ACHKV2_004111"
     CDS             complement(<1..2881)
                     /locus_tag="ACHKV2_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6941893.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQYTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEVNKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKT
                     LSEIQKQELLRNAALIDQQKIREQLRYREETLKNDNVAARASNESELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQAVFYAES
                     DVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAV"
     gene            complement(2929..3138)
                     /locus_tag="ACHKV2_004112"
     CDS             complement(2929..3138)
                     /locus_tag="ACHKV2_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6941894.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(3234..3608)
                     /locus_tag="ACHKV2_004113"
     CDS             complement(3234..3608)
                     /locus_tag="ACHKV2_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6941895.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3623..4339)
                     /locus_tag="ACHKV2_004114"
     CDS             complement(3623..4339)
                     /locus_tag="ACHKV2_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6941896.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(4405..4749)
                     /locus_tag="ACHKV2_004115"
     CDS             complement(4405..4749)
                     /locus_tag="ACHKV2_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6941897.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4746..5192)
                     /locus_tag="ACHKV2_004116"
     CDS             complement(4746..5192)
                     /locus_tag="ACHKV2_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6941898.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(5189..5539)
                     /locus_tag="ACHKV2_004117"
     CDS             complement(5189..5539)
                     /locus_tag="ACHKV2_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6941899.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5549..5875)
                     /locus_tag="ACHKV2_004118"
     CDS             complement(5549..5875)
                     /locus_tag="ACHKV2_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6941900.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5878..8457)
                     /locus_tag="ACHKV2_004119"
     CDS             complement(5878..8457)
                     /locus_tag="ACHKV2_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6941901.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR
                     GTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKT
                     FSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRD
                     AGG"
     gene            complement(8403..8624)
                     /locus_tag="ACHKV2_004120"
     CDS             complement(8403..8624)
                     /locus_tag="ACHKV2_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941902.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(8669..10606)
                     /locus_tag="ACHKV2_004121"
     CDS             complement(8669..10606)
                     /locus_tag="ACHKV2_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6941903.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(10670..12331)
                     /locus_tag="ACHKV2_004122"
     CDS             complement(10670..12331)
                     /locus_tag="ACHKV2_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6941904.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(12328..>12504)
                     /locus_tag="ACHKV2_004123"
     CDS             complement(12328..>12504)
                     /locus_tag="ACHKV2_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941905.1"
                     /translation="DPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPP
                     PPRLVVNNDNEGNG"
BASE COUNT         2530 a         4137 c         3060 g         2777 t
ORIGIN      
        1 ccaccgctcc gcccagccag tcaccaatgc tgccgagaaa tcctcccgca ccggacatac
       61 cgtttgccgc cgtcttaatt ccgttgacaa tcgcggcatt cataagaact tttgatattt
      121 cctgcagtac ggatgaggcc cagctgcgcc attccacttt atttccgttc agcatctccg
      181 tgatgttatt caccatccct gagataccct ccgtcgccag ctgtgctgcc tgtgaggcgt
      241 aatcggacgc attatccacc cagttactga atccctcctg cagccctttc tgccagtccg
      301 cacgctgcac atccgattcg gcataaaaga ctgcctggtc cttaaggcgt tcgctcagat
      361 actgcgcgtt ctgtgccagc gcctgtctgt aaaaatcctc actgatatcc ccggtctgat
      421 actgagactg aaggtccgca tccttctggc ggaagctgtc gcggatctgc tgcaactccc
      481 gcatgcgttc cctggctcgt tccccctgcc cgtaccccag cagttcagat tcatttgatg
      541 cacgcgcagc cacattatca ttcttcaggg tctcttcccg gtaccgcaac tgctcccgga
      601 ttttttgctg gtcaatcagg gccgcattgc gcagcagttc ctgcttctgt atctccgaca
      661 gggttttcag ttcacccagc gctgtctggt acttcagctt cgccagctcc gtgttctgac
      721 cggccagtgc cagttgctct ttctgctgct tcagcagccg ggaaaaactg tcttccgctt
      781 tttccgtctc tgattttcca ccccgggatt tgggtttatt cacctcgtta ttgcgccagg
      841 cttccagggc attactgata taacgttgtc tcgcctcctg atacggatcc cccacaaaac
      901 cgaggtcatc cgccgcatac cccagtcgga cgcgctcttt ttcttcccct ttcagtctgg
      961 acagggccag ctcacgctct gtttttgtca gggcactctg ctgtttatca tccagagtgg
     1021 cctgtggcag ccgtaacggc acattcacca gtccctgccg ctgctgaagc agttcattcc
     1081 ccagccccag cagacggttg aattccgtat actgaccgtt cataaccagc atggactggt
     1141 acaccttatt ctgctctgcc gcctgctgac gaattaacgc cacacgacgg tcttccagcc
     1201 cggcaagcac atcctgaatg gactgcgctt tttcctgcat ctgtgccaga cgggactgct
     1261 caacggcaag ctgctctgtt gcctgagcaa gcccttccgt tacggtcttc accgatgtca
     1321 gatggtttat catgaatccg tcaccggtcg tccagcccgg gttcgccaga acatactgat
     1381 atcctgcgat tttttcctgc agggatttca cccgactggc ctgttcatca atcagccggt
     1441 tctgctctgt cagcgccgcc cgtgttcgtc cttcattatc tgaggcttca ggcaaagaca
     1501 ttgacggcgt tttatgcgcg atttcatcta tcgtcagtgc atactggcgc gcagactccc
     1561 tggcctgctc ctgattctgg tacagcgtgt accatgctgc agcccccagc atcaccagtc
     1621 cgggtacgcc accaaccagc cccagcgcac cgctcatcag acgtgagccc accgccgttg
     1681 tactgttcag cgcattctgg gcggcggttc tggcagcaat atttctgttc aggcgttcct
     1741 gtgtggccgc cagacgggcc tctgcagcaa tctgcatctc cgtcccgcgg gctgccgcca
     1801 cggcctgctg agcacggtac acggctgccc ttgcccgcgc cgtggcaatc tgcgttcccc
     1861 tgaactgtgc ttccgccagt gcaacttcat tacgtgcagc cgtcacaagt cctgccgtgg
     1921 cagacatcgc tccggaggcc atattgccaa agtaccgggc aaccccgacg gcaaccagcg
     1981 cgcccacggc tgttgccaca ttatcaatct gtccggcaac accgttcagc atgccggaga
     2041 gcgtttttgt caccccgctg gcctcattcg caccgcccac ccaggccata aaggcgtttt
     2101 ccacctttgt gatactactg gaaaccgttt ccggcatggc cgcatattca tcacgtaata
     2161 tccccagctg gctgattaac gcggggacca ctttatccgc tgtcagtttt ccgtcatccg
     2221 ccattgcctt cagatcttta cgggccacgc ccatacccgc agccagtgca cgtacgatcc
     2281 ggtcaccact ttcattgacc gaattaaact cctcaccacg caatacaccc tgcgccagcg
     2341 cctgactgaa ctgggtgatc accgagcccg cctctgccgt actggcaccg gagattttca
     2401 gccctgtcga aatggcctcc gtcaccttca gcacatcatc agcactgtaa ccatattcac
     2461 gcatcgaggc agccgaacgg gcaaacaggg ccgcattatc cgaaaatgcg gtgcctgtcc
     2521 gctggctgat atccatcagc actttctgtg atgacgcaaa ttcatcggat gactgtgatg
     2581 cctgtttcag acgggcattc acggagctcc attcatccgc cagcgaaatc aggtgtccgg
     2641 tggcaaaggc accggcaaac gcaccggtca ttccgacagc cgaagcgcgg atttccgtca
     2701 actggctgtg cagctcagcc agagcccggc gctgctccct ggctgccgca gcagcctgac
     2761 gtccgccatt ctgcagggtc cggtaatatt cactgcccat acgggacgcc cgctggatct
     2821 ccgactggaa tgactgtgaa tttgccgaaa ttttgataat cagttcacgt aacgtcgcca
     2881 ttcacctttc tccgggcgta aaaaaaccgc ctcagcggtt ctcatcattc atgactgtgc
     2941 tgcaaggctc agcgcgtctt ccagcgccgc aaacggatcc acctccggct tatcctcatc
     3001 ctcgccccag cagagcatgg cgtccttcag tgcaacattc atcccctgtg ccccgaaaac
     3061 cgctttcacg atctgtgcat tacggatatc cccgcgctca tcacccagcg gggatatcct
     3121 gtcgaactcc atccacatca tcgcctcgct cgcactcagg ctgtggcgca gttcggataa
     3181 ggtgcgcccc agacggagcg caagtcgcat cagaaagcga atttccgggc gggctacttt
     3241 tttctggccg actctgcatc agcgatcagt tccagtgcct gacgcagcaa ccgggcatgt
     3301 accggaccat agacggccag cacctgctca cggtcgtccg gagtgaacac ccgctgcagg
     3361 tccgtatcac acaggacatc gcagaacagc gtcacatccg cttccaggtt acggcgggtt
     3421 ttcgccacca ccgacagggt atcgtcatcc tctccatcac cattgagcac ttcctgccac
     3481 agataccagg cctctgccga aggctcccgc agcaccacgc tgacattacc ccattccggc
     3541 accttcaccg ttttatgacg aaaccctgac agtctggcca gcgccagcgt tttcagatcc
     3601 tttttcatga tgacccatcc ccttatccgg cggcagcgct cactgtcacg gtgcattcaa
     3661 cagacgtcac actctgtgct ttctctgccg aatcggtcac cacacaggta tatttccccg
     3721 catcagcgga ctgcgcacct ggcttactga aggtgtctgt cgtctgcccg tcaaccggct
     3781 gaccatcctt cttccaggcg tatttatacg gcggcgttcc cccgttggca ctgactgaca
     3841 ttgtcagcag cgcaccggta ttcacggtaa gtgtcttatc cagatttttc acaaacgcca
     3901 gcggtaccac aaaggacacc ggtttgcctt tcagacgcag tgagaacgtt gcagccacca
     3961 cgccgttggt accggatgac caggtgtgct ggcgcacttc cgccaggaat ttaaagccct
     4021 taccggacgg aaacagcacc ttaaacgcat acaacgcgtc attgtcatag gcatcacgca
     4081 gggcgttctg ggcctgattc agataaaaat tgcccgacat ggaaatctca gacgacgccc
     4141 ccagaccatt gatgttctcc tgctctgtgg agcagagcgt ggtcacatca atatcctgtt
     4201 tctgcccggc ggtgaactgc acttccttga tggtgcagtc caggcgcaga tattccgcct
     4261 tatccatagt ttcagcagtc gccggggcag atgaaatcat cacctgcgtc agctgtgagc
     4321 gttcatacaa agcagacatt ctgcctcctg ataataaaaa acccgcacgc ggcggggtat
     4381 gggttttgta gaaaaaagaa aaagtcacac cgtgacctga aactccaggg ttgcacggta
     4441 acagcggttt tccggaatat agtcctgcat ttcactgacg gatcccgggg ccagcagcat
     4501 tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc cggcataaac
     4561 gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg ctgacaccga
     4621 tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat ccagcagggg
     4681 gaccacatac gggtacacct gcccgccggc aagatgcgcc agatgaggat acaaatccgc
     4741 ctccgtcatc gtctcagtac ctcatcaatg gcccggttca tccgcgcaat cgccacctgt
     4801 gccgcctgtt cactgcgcac atcaaatgcc gggcgcacaa acgggtgcgg tggcatattc
     4861 acggtcccca tttccacaaa ccgccagtag aaagcattgc gcgggttatc cgccttcatg
     4921 gtgttatcgc tgttaccggt gtccggatta acaccccgga tatgcacacc ggattccatc
     4981 ccgccatcgc gggagcaccg ggaaaggacc accacattgc ggcgaagttt tcccctgcgt
     5041 accggtgccc gtgacaccac ttcttctttc agcacattcg cacccgcacg ggttgcctca
     5101 cgcagcaccc ggttattttc tgcaccactc agaagctgca aatcgcggct gatgtcctcc
     5161 agccccgaaa aatccagcag ggtttcgatc atttttcccc tcccagccga cagagaattt
     5221 ccagacgtcc gccggtcgca tccggcacgg gcagcccgac aacgttcagg atccggtcac
     5281 gccagggacc actcagcaca tgaagtcgtg acgctgccgt gatttcccga ccggactgac
     5341 cgcgcaccca gatgcggatt tccgcctgcg ccatttccgc accggactgc atccgctccc
     5401 ggctgctcct gccccggata tccgcatgaa ttttcccgca tgacacccat tcttccgtca
     5461 tttctccggc agcgttacgg gttaacaccg ggttcagaac acttatcatc tgtgtcagac
     5521 gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc aaatcgtaaa
     5581 tcagcacgga aacagaaaac ggcagctccc cctgaatcag ttcttcccgc tccgcaagat
     5641 ccggattccg gtacagcatc ccggtcagtc gcatggcagc ccccttcatc cgggttaatg
     5701 cctcgcccgg gatcagttca ccgtcctcac gaatcacctt atcccggctg ccctgaatgt
     5761 aggccagcag cacggcggta gcctgacgaa ccttgtccat cagcatctca tcatccgcgt
     5821 catggtcgac acgcagatgt gccttgatct cttccagtgt cagtaatgcc gtcattttca
     5881 gcctcctgca tcccgcccac gttttgcagc cagggtccag gctgatgaat gagcttctcc
     5941 gggtttatct tcggtcatac tgttgcagtg ccacagcgag cccccccacg tcaccgtatc
     6001 gccggggtgg taggtttcac cggctctgaa cacaccgcgg tagagcatca ccggcaggga
     6061 aaatgttttt tccgtacgct ggccactgct ctgccggacc accacagaga acaaccgctc
     6121 acccgtcatg ctgacgtcaa tatccgccac cccgtcaacc aggcattccc atccccgcat
     6181 cccgtgcgtt ttttcatacg cccgccagag tccgccctgg tgtgtggcat acgtgccccg
     6241 gggaaaggat ttttgatcgt caatggcggg gagtatttcc agtgccgtgg catcacgccc
     6301 gtcctgcgga gccggcaggg cactcaccgc atccagaacc gccttctgca gaacatccgg
     6361 atcgtagtca cgaccatcac gcggaacagg aatatggctt accgcctcct tcaccatctg
     6421 ttcaagcatc ggacgcacat catccggggt gagactttta ccgtctgccg gctgcggaat
     6481 atttgcgacc gcatcattca ccgccttctg cagtacttcc ggatcatagt cacgaccgtc
     6541 gcgcggaaca gggatatggc ttaccgcctc cttcaccatc tgttcaagca tcggacgcac
     6601 atcatccggg gtgagacttt taccgtccgc cggctgcgga atatttgcga ccgcatcatt
     6661 caccgccttc tgcagtactt ccggatcgta gtcacgacca tcacgcggaa cagggatatg
     6721 gcttacagcc tccttcacca tctgttcaag catcggacgc acatcatccg gggtgagact
     6781 tttaccgtcc gccggctgcg gaatatttgc gaccgcatca ttcaccgcct gctgcagtac
     6841 atccggatca taatcacgac catcacgcgg taccggaatg gcccccacag cgtcatccac
     6901 catcgcctgc agaaccggac gcacctcatc caccgtcaca tgcttctgta ataccgccga
     6961 cagggaagtc agtttctctt caaacgcttg tgcctgcgag gccatcttcc cctcaaatgt
     7021 gcgctgtaaa tccgccagca ctgtggaaaa ttcttctccc agtgcacgaa taatggacag
     7081 ttcccgttcc gtcattttct cagtatcccc cctgaacatc gctttcaccg catcatgctc
     7141 tgtttcactg attgccttat taccgtcaga tgcgccgtca ggcagctgtg atgaaactgt
     7201 tttcccggca gacgcgaacg gatcctcacg ggcatcacga cgggacagcg cctccagact
     7261 gtagttctgc tgctgaagat acagtgcatc accgccggcc aggggcggca ggttctcccg
     7321 tttacgggcc tcattgggcg tgagaagcgt atttttcacc gcatccccca gcgttttcat
     7381 gcgccgctca ctgtccattc tcagcagcgt ggtgacatca aattctgtac tctcgttttc
     7441 ccccgtttcc agcgcctcat ccagtaacag ttcaatggac tcaatcagcg tctgcaggca
     7501 ctgggaataa tactgctgct ccagcgcctc cacgttgtca ctggaaggcg gttgtcccac
     7561 gccaatcttg taggccggga cacggaacac cgaacagaca atttcagcgg tcatcttcag
     7621 ttgttccacc gtctgcgcat ccaccggtga aaacgtcgtg gggttgtatt ttgccccgtt
     7681 gctcagaatg gccgttttcc ccgcattttc gcctgtatac ccgctgtccc agttgctctt
     7741 cagttttttc gcattttctt ccgtaatact gccggggatc tcaatcaccc cggacggcct
     7801 gccgccattt ctgaaaaaag acgtcgaatt ttcctgaata tgatgcccct gcgtggccgc
     7861 cagcccggcg gcatacaccg gcggcaatcc tataagcgga tgaaaaaaac agttaaaccg
     7921 gtcgtggatc acttcccggg caggcaccgt caccgcctcc gtgatcccgc agttccggtc
     7981 cggcgtgatg cggtagaaca cctcgccgtc atccgccacc agaggttcaa cccggctcca
     8041 gtccagaata cgcagttctt tgatctgccc ccgggcatta cggattttca gcaccaccgt
     8101 attgccatga cgcagtttgg cgttcagcca cagttcaaaa aactggatgc ggttctgctg
     8161 ggcgttggga cgacgacaga ggcgggcaat atccccccgg cgcgtttccc tgcgtatccc
     8221 atgcgcatcc gtctgcataa gacgcagccg cattttggcg atatcctggg atatcagcga
     8281 aatacatgca aacaccgcat gaaaggagag gacggcttca ggatcggctt tcacgccctg
     8341 ctgccaggcg ccggaaaagg gctcagccac cgcctgaaac aggctggtcc agcccgcctc
     8401 tcttacgtca cgtcctgatt tctggttttt tcgggttcgc cgtaaaaggt tccacattcg
     8461 ccatgctccg catcacgttt ctttttctga cctgccggac gtcgcaccgt gatgtactcc
     8521 gcctttccca ggcgaaccag cacctccgca cacggctgtg ccacatcacg gatatccccg
     8581 gcccgggcat catgcgtgcc ctgcagatat cggatctttg ccataacctg ttacgggagg
     8641 cgcacgcctc ccgtcctcct tatcagactc agccgccgga cgcactgccg tagttcactc
     8701 cggtgatcac cgccaccgcc gcagtacggc gacgacgcca gttgatccag cgctccgcac
     8761 ggatggccac gctgcctgtc tggaacatgg aaaccagctc caccggggac ggcgtggtac
     8821 tgtcgccgcc cggctcagac tgcatttcca gtgatgcctc gcgggacata tccactgcca
     8881 cgccgccgtc atccgccaga taaatatccg gggcattcac cagcaccagc tggtcaccca
     8941 cgtactggga gacaatcacc ggcagcccct ggaaggagcc acccagcagg gtcatgtccg
     9001 ggtattcctt ctgacccagc gcatttttac gcatggacag tgccagggca ttggtgctgg
     9061 acatcagcca gaccgcaccg gtgggctgca ggtttgctgc cacaaactgt ccaaacgcag
     9121 cctcggcatc cgcatccggg ttaccggttg atgccgtgcc cttcacatca tgggtgatgg
     9181 acgccgggga gacatctgcc actgcggctt ttttcgggtc cacaaagtct gtatccagac
     9241 gcgccaccac cgcttccgcc agcgcattac ggaccagtgc atcagcagcc ggactggaaa
     9301 aacggatcaa ttcttccgtc agtaccgcaa tggccgacac cttcgcatga ctgaaggtga
     9361 tggattcaaa atcaaacttc gtcaggggtt ttgccttacc ctcacccacc cagccggcag
     9421 caccgccgga cacctgggcg tgcacacgga tattgaatgg cacctgacga agtgcaggga
     9481 tcccgccctg accaaatcgc ccgataatgg tctgcggacg caggtaatca ataaagtcct
     9541 gtgcgtattc ctgatattca gacaggctgc ctgcccactg cggatccgtg gtggtccccg
     9601 cgcccactgc cgatttcagg acatgatgca gacgactgtc atccggatac tgacgacggg
     9661 ccacttccag ggcttcagat cggacgcctt tagccgcagc cagcgatttg gcaaagcggg
     9721 cgaagccaat ccccttatcc agtttctgct ccacacggat caccggcgca gaagccaccg
     9781 cggccacatt cccgttaccg gcctgtttca ccggctgcgc cgtggcggcc ttaccggctt
     9841 ccagttcacg caggcgcttc aggtgcgcat ccacctgacg gatttccgct gcggtgttgt
     9901 cgtaatgctc ttcctcctcc acatccagcg tgcgcccttc ctctgcggct ttggtcatga
     9961 cctcctcaag ggaggctgcc agcgctgcac gcttgttttc aaaactttta atctgttcgc
    10021 caatattcat tatggtcttt tccttatgaa aaacggttgt tgactgtgcc gcagcgccgg
    10081 cagaagatgc gattttcacc accggtttcc ggttgccgga cgcggcagaa aacgggcggt
    10141 cgtaagattt aatggtccgg atggtgcatt ccgcattcgc gggcacggtg acggcagaca
    10201 cctccatcag ttcccagcgc agaaaatgca gtccgcctcc gtccagaaag gtgtattcat
    10261 ggggacggaa gcccacggac agccccctga ccagcccggt cttaatggcc gcccagacct
    10321 catccagccg ggcagccagt tgcgacggca tatccggtac gggcttcacc agtgttgccg
    10381 tgatttccag cccttcgctg acccggcgca ccgtacactg ccccaccggg cgggaatggt
    10441 catgctgcca gagaaacggg atcgcactgc caaactccgc gccctccggc tccaggatgt
    10501 caccatcccg atccggagaa ggcgttgacg caatcccggt gatcacccgt tcatcctcac
    10561 tgaaggattt caccgtcagc agggaacagg cccgtttaag agtcacatca gcctcctgaa
    10621 aataaaaaaa ccgccgcagc ggttcatgat ggttacagtg tgaacagggt tatatgaaaa
    10681 aaaccgcata ttctttcttt ttcggttccg ggttaaggga catcagggag accgcattga
    10741 acagcgccat cagcgggtca atttttcccc gtccactggc ctgtttggtg ataagaatgg
    10801 cgttaccttt aggctccacc cgggcattgc cgacacacca ggccatcagg ggctggtcac
    10861 catgcaccag caccccttca gccagtttgc gctcggtggt tttaatggcc ccgcccagtt
    10921 tccagccctg gcttatcccc accacaattc cgtcggggat cccggcttcc gccagtgaat
    10981 ccagaatctg ccccacccct gacgggtcaa taccgatatg gtccagtaac tcagcctcat
    11041 gaatgcgacg cacatattcc gccacttccg ccgtgtcatc cccgacacgc cggacaatgg
    11101 tcatatctcc acaggcaaca agatcctgaa accgggacgc ctcgctcttc cgtcggacca
    11161 ccgcggtttc atgcgcccag gcatggcccc agcccagcca ttcgcgggtt tccctgtcac
    11221 ggccaatcac gtacattccc agcagatcat ccaggccccc gccgtcaatc cccaccgtca
    11281 ccacatcagc gcgctgcagg atatcgtcca ggctgacgcg cctgccctgc tgctcccaga
    11341 aatccgcacc cgcccagcgg tcagaacgca gggcaagacc aatttccaca ttggcatgtt
    11401 ttgacatgaa gccacgaaat gcttcctcac cagcctcccg ggctttacgg tactcccggt
    11461 acagaaaagc ctcatccacc gaataaccga gattcgggtt aaccatggcg aggttttcca
    11521 tcagcaggtg agccccgctt tccaccattt caggaggatg ctcaaaaatc accggcagaa
    11581 agtgcggatc atgaattttg ccgtcacgga catcccgggc gtactgcagt ttctgtctga
    11641 acaccccggc tggcggctca ttcgactggg tggtcgtata caccacaaac ccttccgggc
    11701 gggaggcaag gccgcctatg gcttcacgta acatgtcctc cgctttgtac tgcttgccaa
    11761 ataaccacag ttcatcaatc agcgtcccca cggacttgat accggatacc gtattcggat
    11821 cggcagccac caccttcagg gtggtgtccg tcaccctgtg ggtgatggtc cggatatgtg
    11881 tctgcacctg acagaggtca tccagatcat cgtcccgtcg taccatatcc ctggcagggt
    11941 tgaaggcgtt agccgccacc tccacggtcg gggccagaat ggtgtagccc gccgcctgcc
    12001 gccagttcag taacagcgcc gtcatcatga tcccggcagc cagcgtggac ttgctgtttt
    12061 tcttggggat aaggataaaa acttccttga tatggcgtac accggtctgc gcatcgtagg
    12121 agccaaacag ggccgccacc aggtcaaaca cccactgtgc gcaggactcc ccgaacgtcg
    12181 ggctgcccgg tgcatccaca atccgcagtt gtttaaaaat cgccagggca tgtgcggcct
    12241 gctccggata aatcggagcc ggaataatcg acagcccctt tttcaggcgc tctgcccagt
    12301 ccgggcaggc cgtgctccac acaggtatca tccgttgccc tcattatcat tattcaccac
    12361 caggcggggt ggtggtggca ccgcaaaacg gttagccgct tttttcgccg cgtcaccttt
    12421 tgccgatttt ttaccggcat cccctttttt gtggtgcgtg aactgcgcca gcttataagc
    12481 cgcatccagc gccagcctgg ggtc
//
LOCUS       DAZFTN010000128        12478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFTN010000128 DAZFTN010000000
VERSION     DAZFTN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..686)
                     /locus_tag="ACHKV2_004124"
     CDS             complement(<1..686)
                     /locus_tag="ACHKV2_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6941906.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQS"
     gene            complement(688..936)
                     /locus_tag="ACHKV2_004125"
     CDS             complement(688..936)
                     /locus_tag="ACHKV2_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6941907.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            1127..1657
                     /locus_tag="ACHKV2_004126"
     CDS             1127..1657
                     /locus_tag="ACHKV2_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941908.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            1910..2521
                     /locus_tag="ACHKV2_004127"
                     /pseudo
     CDS             1910..2521
                     /locus_tag="ACHKV2_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
     gene            2532..3356
                     /locus_tag="ACHKV2_004128"
     CDS             2532..3356
                     /locus_tag="ACHKV2_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO6941909.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3408..4637
                     /gene="sorE"
                     /locus_tag="ACHKV2_004129"
     CDS             3408..4637
                     /gene="sorE"
                     /locus_tag="ACHKV2_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO6941910.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            5020..5709
                     /gene="pepE"
                     /locus_tag="ACHKV2_004130"
     CDS             5020..5709
                     /gene="pepE"
                     /locus_tag="ACHKV2_004130"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO6941911.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5800..7431)
                     /gene="yjbB"
                     /locus_tag="ACHKV2_004131"
     CDS             complement(5800..7431)
                     /gene="yjbB"
                     /locus_tag="ACHKV2_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO6941912.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7651..11334)
                     /gene="metH"
                     /locus_tag="ACHKV2_004132"
     CDS             complement(7651..11334)
                     /gene="metH"
                     /locus_tag="ACHKV2_004132"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO6941913.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11534..12358
                     /gene="iclR"
                     /locus_tag="ACHKV2_004133"
     CDS             11534..12358
                     /gene="iclR"
                     /locus_tag="ACHKV2_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO6941914.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2860 a         3341 c         3266 g         3011 t
ORIGIN      
        1 tttgactggc gatactgcgc cagaaattcg ggcagccagt taatatcttc ggcttttatt
       61 tcctggcgct tacccggtgc cagtaaaagc aggcgttcag cggcgttctg cgccttgtta
      121 aaggccgcaa tccagcgttt cagcgtgatt tcacttaacg cgcggctgtt tccctttttg
      181 gcgttcgccg tctcaaccat tgccacaatg cgctcgtcca gctgcgaacg tgccaggttg
      241 tcaacgataa accggatagc cttcgcacag ctgaaaccag gttgttgcgc gactttcaga
      301 acttcgctga cgatcgcgat tcgtgcatca gccacctggc gctggttttc agtcagggca
      361 ttaaggcgtt cgaccatcag ttgtggtgat ccgcgatatg cctccaccgc atcaaccacg
      421 gcaggtgagc gcctggcctt tgtcactacc ggagccggtg attcatcggc tttttgcgtc
      481 atcagttgca gggcataacg ttcacgtaat gcctgctgcg tcacttcagg taaacagtcg
      541 atgctgtatt cagttgcttt agagcctgag cgacgacggg aacaacagct tttcccttgt
      601 acgtaacgtt gtaatgcctg gcgaatacct tttgttgtag caggcattcc gggcgcgcca
      661 atcagttctt ttgcaatgac atacatatca tgacgccttt ctcatatagc tcttaacctg
      721 gtatctactg ggccagattt cttcaggcga taccccaaga gcatcagcaa ttatttgctg
      781 atacggtcgg caaggagtac gtaatacgct tttcagcgaa tccctgctgt aaccagcctt
      841 taaagataaa gagcgcagtg ataagccagc catgtgtaag cgcgccttga tgatttcagg
      901 cggccaatcg tgatctgaaa cttctcttct actcatactt cctctattct aaaaagttac
      961 ccgcgcggat attcgtgcgg atatctgcac gggtaaataa tgcatcacta aaaataccaa
     1021 atcaaggtat ttttggtatt ttggctaatt tttgctttta tccttatttt tcaattggat
     1081 aaatagaaag aaacctttcc ttagaaaagc accaagaggt atctttatgt cgcagggaaa
     1141 agaacctgtt attgaacgta ttggaatgct tacaaagcgc tacccttcaa gaagtgcagc
     1201 tgccagagcg tggggaatca atatcaacac attaaacagc tatttcaagg gaggacaaac
     1261 acaaccagcc cccagagaaa gtatccttat acgaatttct gaaagtgaag gagttagtct
     1321 tgagtggctt aaagagggca agggtgaatc accaaacgat gttggtattt gtggtaattt
     1381 tggttgttct ggtgataagg gagatcaact tacacagatg ctttcattcc ttacgaaaga
     1441 cgaacgagaa cagttaacca atctccttgc tcgcaaaggt gtagagacgg ttctgacact
     1501 gctggataaa gaaaaccagg atttattaca actagaaggc aggaagcgcc tggctgcgtt
     1561 gatgattgta gatatgccag acgattgcgt cagagagatt ttggaaaaaa ccgagacaag
     1621 taggcaatca aatccggtag aatctaaagc gggttagttt ggcgaggctg gcccgtatgt
     1681 tagttgccat atagatttaa tgaagtttta aacgctttaa aaagttgaga tcgcagtatc
     1741 aaaagttttg ccgattttag aataaacgat taattattgc acaatcggta tcaaatagac
     1801 tttaagcgct catccagaca tcacctgttt tatttcagta tcttacaggt gatttcacct
     1861 cctttcacta aaaccctcgt tggtatcaaa tgattcacct ggttacagct ggatgaacgt
     1921 cggcgcggcg caatctccgg attctgcact cgccagcata atctccgcca ttctggttat
     1981 cgttggtcag caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg
     2041 ccaggtgctg acggtgtttg cccgtaccat caccgtagtg ttccagcacg cggcggataa
     2101 agcagcggaa gaagcacggt ttcgcactct cgatattctg catgtctccg cgcttggcgt
     2161 gcaggcgctg cgcgtcgcca ttccggcact gattgtctcg ctgttcgtca gcgccgatat
     2221 ggtgagcaat atgttaaacg ccattcccga atttgtgacc cgaggtctgc aaattgctgg
     2281 cggttttatc gtggtagtcg gttacgccat ggtgctacgt atgatgggcg tgaaatattt
     2341 gatgcctttc tttttcctcg gtttcctcgc aggtggctac ctcgatctca gtctgctggc
     2401 gttcggtggc gtcggcgtga tcatggccct ggtctacatc cagctaaatc cacagtggcg
     2461 taaagctgaa cctcaactcc tggcccccgc ttccaccgcc cttgaccaac ttgacgatta
     2521 acggagccat catggaacag agaaaaatta cacgcggcga tctggtgagc atgtttctgc
     2581 gctccaacct gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggtttttgct
     2641 acgacatgat ccccgccatc aagcgacttt acccagtaaa agaggatcag gttgcggcgc
     2701 tcaggcgaca cctggtgttc tttaatacca cgccagccgt atgtggcccg gtcatcggcg
     2761 tcactgccgc catggaagag gcgcgggcca acggcgcgga aattgatgac ggcaccatta
     2821 acggcatcaa agtcggtctg atgggaccat tggcaggggt tggcgatcca ctggtctggg
     2881 gaacgctgcg cccgattacc gccgcgctcg gcgcatcgct ggcgctgtct ggcaacattc
     2941 tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg aagtggtatg
     3001 gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc gggaatgtgc
     3061 tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc gtgctggtca
     3121 ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc gccgatggct
     3181 ccaccgtcac catgaccgtg cagaacattc tcgatcaact ttgccctggt ttgctggcgc
     3241 tcggtctgac gctactgatg gttcgtctgc tcaacaaaaa aattaacccg gtatggctga
     3301 ttttcgccct gtttggctta gggattatcg gcaatgcgct gggcttcctg tcctgattct
     3361 tcgccccggc acgactgccg gggccatcgt tcaacatgag gtggtttatg aaaacaacag
     3421 ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt cctgaaatgc
     3481 aggaggatga aatcctcgcg acggtggtca ctgacagcct gtgcctctct tcctggaaag
     3541 aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc aacccaatca
     3601 tcatcggcca cgagttttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat
     3661 tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac cgcccggact
     3721 gccccggcta ctccttcccg tgggtaggcg gcgaggccac gcatgtggtt attcccaacg
     3781 aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt gaaggctcgc
     3841 tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag
     3901 gtagttataa ccacacgatg gggattcgcc cgcaagggcg catgctgatc ctcggcggca
     3961 ccggaccaat gggactgttg gcgattgatt atgcgctaca tggacccgtt aacccgtcgc
     4021 tgctcgtcat taccgatacc gacaacgata aattgagtta tgcgcgcaag cactatccat
     4081 cagaaccgca aacactgatt cattatctca atgccgccga tgcagcattt gatacgctaa
     4141 tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac
     4201 tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc ttcgccggac
     4261 cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc
     4321 actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcagcggtc aaattgattg
     4381 aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg
     4441 gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg tataccggga
     4501 aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg gcgattctgg
     4561 cgcgtcatca ggggatctgg tccggtgagg cggagcaata tctgctcact catgcagagg
     4621 caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg tcaccgcggc
     4681 aacttcgcga cctgatgtgc ggctgattgc tgaagcggcc caatgtgcac gagaaggata
     4741 aaaaaacatc agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc
     4801 cggatgcgga ataaagcctt attcggccta cataatctcg aaaattcaat atattgcagt
     4861 tatctggtag gcctggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca
     4921 gcgcccggtg caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg
     4981 tagcatgcct tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt
     5041 aactcgacgc tgccgggtaa agcctggctg gaacatgcac tgccgctgat tgctgaacag
     5101 ttgcagggtc gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat
     5161 gattacacag cgaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt
     5221 catagcgttg tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc
     5281 ggtaatactt tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact
     5341 gacgtggtta aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc
     5401 ccaaccattc gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta
     5461 aatctgttcc cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa
     5521 ggtgaaaccc gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt
     5581 attggtctac cggaaggtaa ctggatcaca gtgagtaaag gtcacgcaac gctgggtggc
     5641 ccgaatacca cctatgtgtt taaggccggt gaagaagcgg ttccgctgga agctggtcac
     5701 cgtttttaag tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga
     5761 cgcttgacgc gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccctc
     5821 atcttcatca ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcagacg
     5881 ctgcatatct cccagtaagc ctaaatgtag cgaactggtt tcaatgcttt gcacgttttg
     5941 ctgatgcagg cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaagcgatg
     6001 cttgctgcgg cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc
     6061 cagctttaaa ttactgagca attgctcata gagcgcatcc agttccttca agccatcaag
     6121 cgaaaatgct cgccttgcag ccagcgattt gtcggcaatt tcactactca tgcgctcgac
     6181 gatatcggag gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga
     6241 ctcttcttct gccagttcct cttttggcat ccgcgccaga tacagcttaa tggcggtata
     6301 gagaacgttg atatcatctg ccagcttacg cagctctttc tcctgccttg gctcgccgtg
     6361 catcacttta ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc
     6421 gcgcgcggcg ttcgccagag caagcgtggg cgtatccagc gcgctgacat ccagatgttt
     6481 aggccgtagc tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc
     6541 catcgggtca acaaatggca gcatggccag gcaacgtaca aggttgtaga agacgtggaa
     6601 atagatcacc agttccgctt ttggcaatga caacttcccc attgtctctg ccagcaaatg
     6661 gacaaacggc aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac
     6721 acggcgggct gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag
     6781 gttagcacca atcaccagac agagcgccac ggggaaggag ataatgcctg cggcggtcag
     6841 agtcgcggtc agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc
     6901 aatcagcgca tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt
     6961 tgcctgggtg atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc
     7021 aagaccaata ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa
     7081 aatcacgccg ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg
     7141 cgccattagc gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc
     7201 taccagatcc tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac
     7261 cagtgcggta acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct
     7321 aaggacagta cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt
     7381 cccccagacc agcagggcga cggcagaaag caggttaagc agcgttaaca cgagttcagg
     7441 ttctccttat cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct
     7501 gactggcatc tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct
     7561 gtttccagta taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt
     7621 tgttgtaagg aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc
     7681 cagccagcgc tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg
     7741 atcgcgctga atttgcgcca ccgcgtagta cttgctgtcc gggtggctga aataccaacc
     7801 cgaaaccgat gcaccgggcc acatggcgaa agattctgtg agtttcatgc cggtatgttt
     7861 ttccacttcc agcagctccc agatggtggc tttttccgta tgttccgggc aggccgggta
     7921 gcccggtgcc ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt
     7981 ctcgttcggc gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa
     8041 ggcttcggct aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg
     8101 cgcttcaaag gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc
     8161 gatgtaatct gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc
     8221 gaatcccgtt ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg
     8281 cgtttcgtca cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac
     8341 cacgccacgc ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc
     8401 tttaaacagc cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt
     8461 cccggccagc gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagagtttc
     8521 gatgctggct tcaacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg
     8581 ccagtcaaaa gcgaagtcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg
     8641 tttcttgcgc ccgtgctgaa tacgtacggt ttcgtactcc ttgcgggtac gagcgacaaa
     8701 atcatcgcgc tgagtatcgg aaagcagcgc cgccaccaca ccaacggtgc gcgaggcatt
     8761 ctgcacatac accgtcgggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt
     8821 tgaggtcgtc gcgccaccga tcagtaacgg aatggtgaag ccctgacgct ccatctcttt
     8881 cgccacgtta accatctcgt ccagcgacgg cgtgataagc cccgaaagtc caatcagatc
     8941 agcattcact tctttagcgg tacggagaat tttttccgca ggcaccataa cgccgagatc
     9001 gacaatttcg tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg
     9061 gacgtcgccc ttcacggtgg cgatcaccat cttgccgttg gttttgccct gctctttgct
     9121 ggcttcaata aacggttcga ggtaggccac tgcctgcttc atcacacgcg ccgatttgac
     9181 cacctgtggc aggaacattt tcccttcgcc gaacagatcg ccgaccacgt tcatgccgtc
     9241 cattagcggg ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcggt
     9301 atcctgctcg ataaactcag taatgccttt gaccagcgag tattccagac gtttattcac
     9361 ttcccacgag cgccactccg cctgctgggc gttagcggtg tcgtcggttt tgctgccgcg
     9421 atatttctcg gcaagctcca gtaaacgctc agtgccatca tcgcgacgat taagaatcac
     9481 atcttccacc gcgtcgcgca gttcagcggg caggtcgtcg taaatcgcca gttgcccggc
     9541 gttgacgatc cccatatcca tgccattgcg aatagcgtag tagaggaaca ccgcgtgaat
     9601 ggcttcgcgc accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga
     9661 aatcagcgcg tgcggcagtt cgcgtttgat gtcttcgcag gcgccgataa agtcctgcgc
     9721 atagttgttg tgctcttcaa tgccggtggc gaccgcgaag atgttcgggt cgaagatgat
     9781 atcttctggc gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc
     9841 aattttccgt gcgcgggtat cggcctgccc ctgttcgtca aaggccatta ccaccaccgc
     9901 tgcaccgtaa cgacgcaaca gtttcgcatg atggataaag gcgtcgacgc cctctttcat
     9961 tgagatagag ttaacaatgc ctttgccctg aatacacttc agcccctttt cgatgacctc
    10021 ccattttgag gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag
    10081 attgagaaaa cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc
    10141 gataatctgc gcgccgtttt ccacctgctg acgcgctaca tccagcgcct cgctgtattt
    10201 ctcttctttg atcaggcgct tgaacttagc ggaaccggtg acgttggtgc gttcacccac
    10261 gttcacaaac aggctatcgt cgccaatgtt cagcggctcc aggccggaca aacggcaggc
    10321 tacgggaatt tcaggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc
    10381 aatatgttgt ggcgtggtgc cacagcagcc gccgacgata ttgagaaaac ccgcttgcgc
    10441 ccattcacgt atctgttttg ccatcgtgtc ggcgtcgaga tcgtactcac caaaggcgtt
    10501 gggtagcccg gcgttcgggt gcgcggtgac gtagcattcc gcaatccgtg atagttcctg
    10561 cacgtactgg cgcagttcat ctggccccag cgcgcagttg aggccaaaag tcagggcttc
    10621 ggcatggcgc agggagttgt aaaatgcttc ggtggtctgt ccggagagcg tgcgcccgga
    10681 ggcgtcggtg atggtgccgg agatcataat cggcagctca acgcctaacg cttcgaactc
    10741 cgttttcacc gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag
    10801 aatcagatcc gcaccgcctt ccactaatgc tttggtggat tcgcgatagg ccgccaccag
    10861 ctggtcaaaa gtgatgttgc ggaatgccgg atcgttcacg tccggagaaa tggacgccgt
    10921 gcggttggtt gggccgagaa cgccggcaac gtagcgcggt ttctccggcg tgcgcgcggt
    10981 ccactcgtca gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag
    11041 ggattccatc tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat
    11101 gatatccgcg cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttgct
    11161 tagcaccagc agatcgttat tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc
    11221 acgaaaatcg gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccag
    11281 caccagaata cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc
    11341 tcccgacacg ctcaacccag atttaataaa aattcaacaa accatactgg cataaacgca
    11401 tctgtggtaa aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc
    11461 agttcagcaa ctattgcatt agctaacaat aaaaatgaaa atgatttcca cgatacagaa
    11521 aaaggagact gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc
    11581 caccgcacca gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg
    11641 gattgccgaa tccaatggca gtgtggcact cacggagctg gcgcaacaag ccgggttacc
    11701 caattccacg acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt
    11761 tggcgaactg ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct
    11821 ccagagccgt aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc
    11881 tggcgaaacg gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga
    11941 ccaggtacag tgtacgcatc tgatgcgaat gtccgcgcct atcggcggta aattgccgat
    12001 gcacgcttcc ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa
    12061 gctgctgcac cgcaaagggt tacatgccta tacccacgca acgctggtgt ctcctgtgca
    12121 tttaaaagaa gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca
    12181 tgcgctgggg ctacgttgcc ttgcagcgtg tattttcgat gagcaccgtg aaccgtttgc
    12241 cgcaatttct atttccggac cgatttcacg tattaccgat gaccgcgtga ccgagtttgg
    12301 cgcgatggtg attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact
    12361 ttttctggcg ggcagaggca atattctgcc cgtcatacct gagtggtaat agaataaggg
    12421 tgtctgttaa tcgcattgac gtcaaaataa cttattgtca tacacttcat acactttt
//
LOCUS       DAZFTN010000129        12184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFTN010000129 DAZFTN010000000
VERSION     DAZFTN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..269
                     /locus_tag="ACHKV2_004134"
     CDS             <1..269
                     /locus_tag="ACHKV2_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6941915.1"
                     /translation="GPQGPKGDPGETQIRFRLGPASIIETNSNGWFPDTDGALITGLT
                     FLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            271..540
                     /locus_tag="ACHKV2_004135"
     CDS             271..540
                     /locus_tag="ACHKV2_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6941916.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            826..897
                     /locus_tag="ACHKV2_004136"
                     /pseudo
     CDS             826..897
                     /locus_tag="ACHKV2_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(908..1156)
                     /locus_tag="ACHKV2_004137"
     CDS             complement(908..1156)
                     /locus_tag="ACHKV2_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6941917.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(1384..1449)
                     /locus_tag="ACHKV2_004138"
                     /pseudo
     CDS             complement(1384..1449)
                     /locus_tag="ACHKV2_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1671..3356
                     /gene="btsS"
                     /locus_tag="ACHKV2_004139"
     CDS             1671..3356
                     /gene="btsS"
                     /locus_tag="ACHKV2_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO6941918.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3353..4072
                     /gene="btsR"
                     /locus_tag="ACHKV2_004140"
                     /gene_synonym="yehT"
     CDS             3353..4072
                     /gene="btsR"
                     /locus_tag="ACHKV2_004140"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO6941919.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            4119..4589
                     /gene="yehS"
                     /locus_tag="ACHKV2_004141"
     CDS             4119..4589
                     /gene="yehS"
                     /locus_tag="ACHKV2_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO6941920.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4631..5092)
                     /gene="yehR"
                     /locus_tag="ACHKV2_004142"
     CDS             complement(4631..5092)
                     /gene="yehR"
                     /locus_tag="ACHKV2_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO6941921.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5217..7220)
                     /gene="yehQ"
                     /locus_tag="ACHKV2_004143"
     CDS             complement(5217..7220)
                     /gene="yehQ"
                     /locus_tag="ACHKV2_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO6941922.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(7217..8353)
                     /gene="yehP"
                     /locus_tag="ACHKV2_004144"
     CDS             complement(7217..8353)
                     /gene="yehP"
                     /locus_tag="ACHKV2_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6941923.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(8346..10625)
                     /gene="yehM"
                     /locus_tag="ACHKV2_004145"
                     /pseudo
     CDS             complement(8346..10625)
                     /gene="yehM"
                     /locus_tag="ACHKV2_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(10636..11724)
                     /gene="yehL"
                     /locus_tag="ACHKV2_004146"
     CDS             complement(10636..11724)
                     /gene="yehL"
                     /locus_tag="ACHKV2_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO6941924.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2915 a         3076 c         3246 g         2947 t
ORIGIN      
        1 ccggacctca gggaccgaag ggcgacccgg gggagacaca aatccgtttt cgtctggggc
       61 cggcgagcat tattgagaca aacagcaatg gctggttccc ggatacagat ggcgcactca
      121 tcaccggact gacctttctt gaccccaaag atgccacaca ggttcagggg ctgtttcggc
      181 atttgcaggt caggtttggt gacgggccgt ggcaggatgt taaggggctg gatgaagtgg
      241 gcagtgatac aggcagaaca ggagaatgac atgaatatac taaaaaaact tatgcagcgt
      301 ctgtgtggtt gcggaaagca tgatggccgt gaacacgtgc agtcgcttac agcacaactg
      361 cgactggggc cggcagacat cctggagtcc gatgagaatg gtattattcc ggagcaggac
      421 agggtaatca cgcaggtggt gatactggat gcggataaaa agcagataca gtgcgtggta
      481 agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa atattggcgg gatgaagtaa
      541 cccgacagct tcacaaaacc ggagtccggc tccggttttt gtgttgcaat gtccggggga
      601 tatttgttag gtagattgta ttgtatgtaa aggcagagct ccgaatttta ctgttgtaag
      661 tgggcctggc gggaatggta tcgccagcat cattggcttg ggtccagtaa aaagagacgc
      721 agaaagatgc acaataggca ctacgcgtca tgcatggatt aggtcgctca ttatttacac
      781 agtactgcta aaaaattatt aaggaaagcg cggcacttcg tatgtaagag cgtgtcgcgg
      841 ttggctggtg aactttcgat agtgcgagta ttgaatgatt tccagccgtt accgatttac
      901 gtgtttatta gcgaacaaac cactcgtcag cagactccca ggtatctttc agcgtctcct
      961 gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg ccatcgttgc
     1021 tggtggcttt tacgatcacc tctacatcgt cataacgttt actgatgcgt cgggttaatt
     1081 cttcctttaa cgcatccaca gcaccgtttg gcattttagt gattttttct ttagcgatac
     1141 agatctcaac acgcataaca ccctcctgta actgtagtta tgtacaggtg ttatttttat
     1201 ctgtatggat aaccagtgtc aattcctgat attgtttatg gggcatcaat ggggcatgta
     1261 tgggacactt tttatcggcg aaattcgtcg aagttcatcg acatggtaaa cgaatcatct
     1321 atccaaccct tgaaaaacgg cgctcctgga cgatcttcgt cgatttttaa aaatgttgcg
     1381 tcacgcgcgt aacgtgacag ggttaatatc acaaagcaac gccacttcac caattgtgta
     1441 aagcgccatc gtctcaccct tgctcgcgag gtcccggttt aactttagac gcagttttgc
     1501 gaaccaggta gttttgcccg ttttttgtgc atttataggg tgattttatt tttgccaggc
     1561 gattttgagt gatcgtactc acgaattctc atttttctgc aagagttcaa agaaagttaa
     1621 acgcaggcaa tgtatgttac gcgttttaaa gggatgtgtg gtttgcgggt atgtacgatt
     1681 ttaatctggt gttgctgctg cttcagcaga tgtgcgtttt tttagtcatt gcgtggttaa
     1741 tgagtaaaac gccattattc ataccgttaa tgcaggtcac ggttcgtctg ccgcataaat
     1801 ttctctgcta catcgtcttt tccatcttct gcatcatggg cacctggttt gggttgcaca
     1861 ttgacgattc tattgccaat acccgtgcga taggcgcggt aatgggcggc ttactcggcg
     1921 gtccggtcgt cggtgggctg gttggcctga ccggcgggtt acatcgatat tcgatggggg
     1981 gcatgaccgc gctaagttgc atgatctcga ccatcgttga aggattgctc ggcggcctgg
     2041 tacacagcat cctgatccgt cgcgggcgca ctgataaagt ctttaacccc attaccgctg
     2101 gtgccgtcac gttcgtcgct gaaatggtgc aaatgctgat catcctggcg atcgcccgac
     2161 cttatgaaga tgcggtgcgt ctggtgagta atattgctgc gccaatgatg gtcaccaata
     2221 ccgtcggcgc ggcgctgttt atgcgtatat tgctcgataa acgcgcgatg tttgaaaaat
     2281 acacttcggc tttttctgcc actgcgctga aagtggcagc ctcgacggaa ggcattttgc
     2341 gccaggggtt taacgaagtg aacagcatga aagtggctca ggtgctgtat ctggagctgg
     2401 atattggtgc agtcgcgatt accgatcgag agaaattgct ggcctttacc ggaattggtg
     2461 acgaccacca tttacccggc aaaccgattt cttcaactta tactttaaaa gcgattgaaa
     2521 ccggtgaagt ggtctacgct gatggcaacg aagtacctta tcgttgctct ttgcatccgc
     2581 aatgcaaact ggggtcgacg ctggtaattc cgttgcgtgg tgaaaatcag cgggtgatgg
     2641 gcaccatcaa attgtatgaa gccaaaaacc gtttatttag ttcaatcaac cgcacgctgg
     2701 gcgaggggat tgcgcaactg ctttcggcgc agatcctcgc cgggcaatat gagcggcaaa
     2761 aagcgatgct cacccagtca gagatcaaac tgcttcacgc ccaggtgaac ccccattttt
     2821 tgtttaatgc gcttaacacc attaaagcgg tgatccgtcg cgacagcgaa caggccagcc
     2881 agctggtgca gtatctttcc acttttttcc gcaaaaactt aaagcggcct tcggagtttg
     2941 ttactctcgc cgacgaaatt gaacatgtga atgcttatct gcaaattgaa aaggcgcgct
     3001 tccagtcgcg gttgcaggtc aacattgcta ttccgcaaga attatcccag cagcaattgc
     3061 ccgcgtttac cctgcaaccg atagtggaaa acgccattaa acatgggaca tcacaactgc
     3121 tggatacagg gcgagtggca atcagcgtcc gacgtgaggg gcaacatttg atgctggaga
     3181 tcgaagacaa tgccggtttg tatcaaccgg taaccaatgc cagtgggctg gggatgaatc
     3241 tggtggataa gcgtttacgt gaacggtttg gcgatgacta tgggataagc gtcgcctgtg
     3301 agcctgatag ttacacccga ataacgttac gactaccatg gagggacgag gcatgattaa
     3361 agtcttaatt gtcgatgatg aaccgttagc acgggagaac ctgcgcgtat ttttgcagga
     3421 gcagagcgat attgaaatcg ttggtgagtg ttcaaacgcc gtggaaggga tcggcgcggt
     3481 gcataaactg cgcccggatg tgctgtttct cgatatccag atgccgcgca tcagtggtct
     3541 ggaaatggtg gggatgctcg acccggaaca tcgtccgtat attgtttttc tcaccgcgtt
     3601 tgacgaatac gctattaaag cctttgaaga acatgccttt gactatctgc tgaagccaat
     3661 tgatgaagcg cgactggaga aaacgctggc gcgattgcgt caggagcgca gcaagcagga
     3721 tgtttcgctg ttaccggaaa atcaacaggc gctgaaattt atcccttgta cggggcatag
     3781 tcggatttat ttgctgcaaa tgaaagatgt ggcatttgtc agcagtcgga tgagcggtgt
     3841 ctacgttacc agccacgaag ggaaagaggg ctttaccgaa ttgacattac gtaccctgga
     3901 aagtcgtaca ccactactgc gctgccatcg tcagtatctg gttaacctcg cgcatttaca
     3961 ggagattcgt ctggaagata acggtcaggc cgagttgatt ttgcgtaatg gcttaaccgt
     4021 gccggtcagc cgccgttatc tgaaaagctt aaaagaggcg attggcctgt aaaagactgc
     4081 taaaatggct ttttgcctca tcaacacctg aaggcctcat gctaagtaac gatattctgc
     4141 gcagcgtgcg ctacattttg aaagccaata ataatgacct ggtgcgtata ctggcgctgg
     4201 gtaatgtcga agccaccgcg gaacagatcg ccgtctggct acgtaaagaa gacgaagagg
     4261 gttttcagcg ttgtccggac attgttttgt cgtcattcct caatggcctg atttatgaaa
     4321 aacgcggcaa ggatgagtct gctccggcac tggagccgga acgtcgcatt aataacaaca
     4381 tcgtgctgaa aaaattacgc atcgcgtttt cgctgaaaac cgatgacatt ctggcgatcc
     4441 tcaccgaaca gcagttccgc gtttcgatgc cggaaattac ggcgatgatg cgtgcaccgg
     4501 atcataaaaa cttccgcgaa tgcggcgatc aatttttacg ttattttctg cgtggactgg
     4561 cagcgcgcca gcatgtgaag aaaagctaag acgggtatgg cggccatgcg caaacatggc
     4621 cgccgacaga ttatttcact tctttaaaac cagcggcttt catcaccagt tccatttgcg
     4681 ccatagtgat accttttttg gcatcttcag cagaaacgtt gattcctgaa ataccctgca
     4741 gggctttaaa atccactttt tccatatcga tagtcacgtt ttcctgcgcg taggtatcgg
     4801 tataggttaa tttttcttca acacccgcga tgtttttgta tttggcgctt aacggctcaa
     4861 gtgtcttggc agcatcttct ttggtggtag caccaatgga ggcaaattga attttggttt
     4921 cagaagattg cttaagcacc ttgtcacctt tgtagacata ggtaatggca atttcagtgc
     4981 cgttcagatt ggcgctgaat ttcttcgatt cttctttgtc accgcagcca gcaagagaaa
     5041 aaaccagaac agatgcaaca acaagggaaa acagcttatt gaaagccttc atgtaaaact
     5101 ccattttatt taatcaagga actggtgact ctcaccaggg gctatatagg atatgcctaa
     5161 taccgtggcg tgagcagtcc ggaactggag tagaactctt agtaaaaagc actatttcat
     5221 ccttgttgct gaagcagggg gaataattgt tcgcacagca aaacaccgtt attcattgct
     5281 tctacccgtg cctcgctttc tgtattacga aattgtccca atacatgtgc cagccgataa
     5341 aaacccaccg cggtgaggtc attcgccagc aactctgcct gaccaatagc actctgttcc
     5401 tgatagcgcc agccgttatg gagcagttga ataagtaatg cctggcagcg catcagcaac
     5461 tgatgagcag tagacggcac aggcagaacg ctggcagaag gtagtgatgc caccacaggc
     5521 gcagtttctg cgtccagcgc ccaggcacga gtctttgtca tcattacctg tggttccagt
     5581 gtcaattgcc catcaacaaa actgacaaag ccagaaacca gacacacggg gtcgtctgtt
     5641 tgttgcaaaa gcgccgccat gcgttcaacg gcataaggcg cgctggctga ggctggcagt
     5701 gataacgtca gcagattatc ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg
     5761 cggctgctat cccaaccgag cgaaatacac tcagcgaccg gcagaataaa taagttatcg
     5821 acctgattaa gaggccgtat gcaggagggg ggacgctggc gtaaatattc ccgcaaagcc
     5881 acaatgcccg gctggcgtaa cggcgcgctc aacattcgcc aggcatcagg cgacagcggc
     5941 acaacgctgc ttaagcggtt gcgggtagct aacagcagct cgccatcggc actgcgtttt
     6001 gctgcttgtg aaacaatttg cccacccgcc agtgcgccag cctgaaaact aaacagccga
     6061 cgcgtagctg ccggtgagtt ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa
     6121 atactgccgg tgtcgggatc ggtaaaccag atgcgtaaac cataatgctc aatatcctgc
     6181 cagcaacgca tacctaaaga caccagccgc agatgatcaa gctttgcttc tccggcaatg
     6241 ccagagccaa cgaccgtgcg ccacggcaca ggaggaactt caccaacact gtcgcgccgg
     6301 gccatctctt gtgcgcaatt taatcgactg tttaatgccg caagctgacg taagcattct
     6361 cctgcatgat agtggctggc gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt
     6421 agcgattcac tcacccagcg ccagttgcag cgttccgccg cttgctgcgc gcggctgaaa
     6481 gcggcctcgt agtgaataag cggctggctg atgccgccaa gccataatgc ctggcttaat
     6541 tgctgaacat attgacgaca cgcgttgcct tcttcgctgg caaacgggtc gtcagatgat
     6601 gtgacgtgtt cgctgcgcat ctgccagatt aaatgagtaa attccgcttg ctgagttttg
     6661 gcctcgacga aggcctgcac agccagtacg acatgttcgc aaagtgtgcc ttcaatacaa
     6721 tcacagcggg cgaagcgaat gctgctgcgg gaataaaaac gcacatcact catcggtaag
     6781 cgggctgagg gaatttcgcc aggcgcacag aacaactcaa tggtgatgcc tttagcgacc
     6841 agcacctgtg cacgtttgcg ggtggcatcg ggcagggtag ccagttcttc cagccagatt
     6901 gcaggctgcc actcttcttc tttttccgtg ggccgggtgg tggcacaaag tcgctgataa
     6961 cttaacacca gcatcacgcg atgacggcac atgccgctag ccccgcaggt gcactgagcc
     7021 tctttcagtg cctggccgtt cgccagctgg gtacggacac cgtcactgaa ggtggcgatt
     7081 aaagcgccgt tctcatggct gatttccggg acgttgccat tttccagttc cttaaggctg
     7141 cgcttaacaa aaccggcatt gcttaacgcc gtcagggcct gcggcgtgag ttctaataat
     7201 tccggacgta gtgaattcat gactgcaaat tctccgcaag ccaggtagcc agttcacccg
     7261 gtgtcattgc ggctatttgt gcgccaacat taaccagcgc ctgggccata tcgcggtcat
     7321 agcaaggcgt tgcggtgctg tcgagcgctg ccagccccag cactttgatg ccgctctgga
     7381 cacacttttt cacctgatgc gtcagcaatg atgatgaacc cccttcataa aaatcactca
     7441 cgaggataat gacgcttttt gctggttgtt caataagttg ccgcccatac tccacggcac
     7501 tggcgatatt ggtcccgccg cccaactgta ctttcattaa taactctacc ggatcggcaa
     7561 cgtctgccgt gagatcaacg acactggtgt caaacgccac cagatgggta cgaatgccgg
     7621 gtaactgcca caaacaggcc gccattaccg cagagtggat caccgaatcg accatcgatc
     7681 cgcttagatc aaccagtaag accagttgcc attgttcact gtggcgctta attcggctgt
     7741 taaagcgggg ggattcgata tacaacttgc cgtgttgcgg gtgccagtgt tgcagattgg
     7801 cgcgaagagt acttttgaaa tcaaagtctc gcgccagtgg aatagatgag cggcgacggc
     7861 gatcgcggac accagaaaaa gcctgacgaa cttcctttgc cagtcgagcc ataatttctt
     7921 caacaacctg gcgcactatc cggcgggcgg cagccagtac ttcggggttc atcagatgtt
     7981 tggtgtgcaa aacggcgcgt agcaggcttt cagaaggctg catacgttcc agcacgtcga
     8041 gattcgtcac cacatcttca atgccgtagc gcagtacggc atcgctttcc agccgctcaa
     8101 tcacctgttg cggaaacagc gtgtgaatac tgttgatcca ctcaggggtg gtgagatttg
     8161 agccacctaa tccaccggag cgttcaccac gctggagccg ttcaggatcg cgcccataca
     8221 gccactccag cgcgtggtct atctgccggg cgttgtcatc cagcccacaa agcgtcgttt
     8281 ctgccgcttc gccaagaatt aatcgccagc gttgtagctc acgggtggtc agaagatcgt
     8341 tcagttcaga catggaaaac tccccagttt tgcagcgatg ccagtgcctg ctgttcgagt
     8401 tgttgatgat gtgcaatggc ttgtggtggg caatgcaacg gcatttgcag cgccgaaacg
     8461 ggaagttgcg ccagttgata atgttcaagc acctgatgcg ccagtgtccc gcgttctcgt
     8521 ggcggtagcc aggccatcgc cgcgcgtaaa tcgggcaggg cattgataaa atcggcatca
     8581 cttggttgat ttaaatgact gctgaaaccg gcgataaatg ccggctgaca ggccagttga
     8641 tggcgggcca gcgccagcaa accgtgcagc gcttcgccgg attgtgccgg ggataactgc
     8701 gccagcatcg tcagcgccgc ttcggcacta gcgttgggat gttccagacg catcagtgcg
     8761 ccaagagccg cgccgcgatc cagcgccggg gcatggattg cctgactgcg tcgctccagt
     8821 aaggctaccg ccgcagaaag agaaacgccg ggtaaattaa cgccgctatg tagatcgcgc
     8881 agaatatggc aaagcgcttg ccagctatgc aggtgagcgt gaaactcctt ttcatccggt
     8941 ctgccgttag attcacacag ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt
     9001 gatatctgcg caccttgcat accgctaatt tcatccagcc gccataaggc atatagcact
     9061 tccagcgccg ggcccatttc ggcaaattga ttttcctagg cgattaataa tgtgagttgc
     9121 tccagtaatt gttgactgaa ggacgccaga cccgctaacg ccgcctggct aagacatgtg
     9181 gtgatactgc cgattccacc cgcgtccagc atatcggctt ctaatttatg gcgtgcggct
     9241 tcctggagtg tggcaccaaa gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg
     9301 cgggttaatt tccagcgttc ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg
     9361 cgtacaatcc ctgggatctc cagtattgcc agccgatgta acacctgact ttgcgctagt
     9421 ccattggggt taaagcgatt taaggtaagc tccgccgaaa gagagattgc ggtacgttcc
     9481 agttcgcagg tgacatcttt tggcagaggc ggttgtggcg tagaaggggc aagtttaccg
     9541 aatccgtcac ccgctaatgt gtctatcaac gttagcagaa tcggatcggt atcgggatga
     9601 ataacgccgc gatagctcca cggcaacggc gcgttcaggg cttctttaat cagcgagcct
     9661 gctatggcat ccagccagtc agtgcgtaac ggtagtttat atccgcgcaa ctgtgccagc
     9721 gccatcgcat gcagatgagc ggcagccata tccgcggtcg aagcaggcaa gtggtgttgg
     9781 cgcaaacggg taagaaccgt ttttagcagt tgttctcccg cctgctgtaa gccccactgc
     9841 cagcaccagc tttgccagac cggggcaggc attcctgaaa ggtatcctgc cagcacatca
     9901 aggcgctttt cactgtaggg tgtgagataa caacctgtaa cggcatctgc cagcgagggc
     9961 aattctggct tgttaatgtc ctgcgggcat tcgcgccaca tttttgccag tgccggagcg
    10021 tgccagccac cgcagacgac taacacgtcg ccattatttt gctgcactgc ccatgcaatc
    10081 cagcgagcca taaaggcttc gcgctgacga ttgagtgcat cgccggggaa atcgccccgc
    10141 agttgggcaa aatagtgcgc cagtgcagag ggtaatgcag tttgctggct ttcatcttcg
    10201 aacaagtgat cccacagggt atcgctgtta tccatgcggg tggcacgcag cagcaaggcc
    10261 tggctttcct cttgcgtatc aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc
    10321 aaatcgataa agtaagtttg tgcctgaata tgacgtgcgg cttgtagcgc ctgccactcc
    10381 ggcgaaaatt cggcaaatgg cgtccaggcg ccgcgcccag gagcagcacc gtcctgatac
    10441 tggcaataac tgtaaatagc caccggaagc tggtgggcta aaaacagttc atctacccgg
    10501 tcattaaaat cagccgggcc ttcaatcaac acgtatcgtg gtcgctggct ttcgattaac
    10561 gatttcacca gtcgggcgca ggccggacta tgatgccgga tgccgacaat taacggctcg
    10621 ctcatgcatc tttcctcacg gcaggcggtg gcgggcttga taataagcct gccagtgagc
    10681 ttctttatgt gtggcgacac gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc
    10741 gttatctttg acgatggttc cggcaataca gtccaccagg tcggcaggct cccccgcgcg
    10801 attcgctaaa aaccaggcgc ggacgcccac agcgtgggcc acgttcacgg cttcagcggt
    10861 ggacataatc gctgttagtg tatccattga cgttttcttt tcgccgttgg cacgcagatc
    10921 gcggaaggtg cggaccagta actccagtac cgcgtcaggc actttatgcg gaataccgct
    10981 atgggccagc aaacgcgccg aagcactggc gacaagctcc agctcctggg cgaaatccat
    11041 aatcggaaag acagtttcaa aatcgaagcg acgctttagc gcagcactca tttcattgac
    11101 gccgcgatcg cgggtattgg cagtggcaat aatattaaag ccttctcgcg catagagctg
    11161 actggcttca ccagtgagtt ccggcaccgt catcaccctg tcagaaagca tacctaacag
    11221 acagtcttgc acttccagcg gtgtacgcgt tatctcttca aaacggacga ttttgccgtc
    11281 gcgcattccc tggtaaagcg gggcagggac cagcgcttcc gttgatggac catgattgat
    11341 aagcagggcg tagttccagc cgtacttgat ttgatcttcg gtagtagatg cgccgccctg
    11401 gatggttaac ccggcatcgc cgctaataga ggttgccagt aattcagaca ggagagattt
    11461 tgcggtgcca ggttccccca ccaacatcaa accgcgtccg gtggcaagag tgactaacat
    11521 acgttcgaca gcggagggac taatcaccac tttacggcta atattttgcg cgctatcgcc
    11581 aaggataaaa gcacgggccg caggcaaact taactgccaa ccgggcgggc aaggtgcgtt
    11641 atcattttgt tttaattttg ccagttcatc ggcgtataac acagcagcag gcggacgctg
    11701 cagatgatta ttctgcggtg acatatattc atccctgaaa tgtattaatg aaataaacga
    11761 tattttacgc gcaataaatg tttggcgcat tagttgtttg agcgaaaaat atctgggaag
    11821 cagatcacag taaataaaaa aacctgctgg tgaggcaggt tcttttattt actattttca
    11881 ggcctaacac cacagcctgt gcaacatcgt gttccaacga acgaattata accacggctg
    11941 gaccccgttg caattaagat aatgagattt ataatcctga ctgttgggac gttgcgccgg
    12001 atcgaaccta tttcaagtcc tggctaccgg acggggggtg ctgcaggtgg tgccctgatc
    12061 cggagtgcaa catcgtggtc caacatacgt aaaccagtat cgacaggcag tgtaagggta
    12121 aggtgctggt gttgcgctcc ttaataagga gagcggatgg actcgctgac acaaaagtta
    12181 accg
//
LOCUS       DAZFTN010000130        12177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFTN010000130 DAZFTN010000000
VERSION     DAZFTN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            206..976
                     /gene="yjjP"
                     /locus_tag="ACHKV2_004147"
     CDS             206..976
                     /gene="yjjP"
                     /locus_tag="ACHKV2_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO6941925.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            967..1440
                     /gene="yjjB"
                     /locus_tag="ACHKV2_004148"
     CDS             967..1440
                     /gene="yjjB"
                     /locus_tag="ACHKV2_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO6941926.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1547..2086
                     /gene="dnaT"
                     /locus_tag="ACHKV2_004149"
     CDS             1547..2086
                     /gene="dnaT"
                     /locus_tag="ACHKV2_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO6941927.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2089..2826
                     /gene="dnaC"
                     /locus_tag="ACHKV2_004150"
     CDS             2089..2826
                     /gene="dnaC"
                     /locus_tag="ACHKV2_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO6941928.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2875..3369
                     /gene="yjjA"
                     /locus_tag="ACHKV2_004151"
     CDS             2875..3369
                     /gene="yjjA"
                     /locus_tag="ACHKV2_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO6941929.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3623..5914
                     /gene="opgB"
                     /locus_tag="ACHKV2_004152"
     CDS             3623..5914
                     /gene="opgB"
                     /locus_tag="ACHKV2_004152"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO6941930.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6051..7073)
                     /gene="lgoD"
                     /locus_tag="ACHKV2_004153"
     CDS             complement(6051..7073)
                     /gene="lgoD"
                     /locus_tag="ACHKV2_004153"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO6941931.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7212..8126
                     /gene="lgoR"
                     /locus_tag="ACHKV2_004154"
     CDS             7212..8126
                     /gene="lgoR"
                     /locus_tag="ACHKV2_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6941932.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8341..9702
                     /gene="lgoT"
                     /locus_tag="ACHKV2_004155"
     CDS             8341..9702
                     /gene="lgoT"
                     /locus_tag="ACHKV2_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6941933.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9751..11415)
                     /gene="tsr"
                     /locus_tag="ACHKV2_004156"
     CDS             complement(9751..11415)
                     /gene="tsr"
                     /locus_tag="ACHKV2_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO6941934.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11644..>12177
                     /locus_tag="ACHKV2_004157"
     CDS             11644..>12177
                     /locus_tag="ACHKV2_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941935.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEINNTPYCYI"
BASE COUNT         3014 a         3169 c         2919 g         3075 t
ORIGIN      
        1 taaattcatt atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc
       61 tttttatcac cgagcttgtc ccgataacca ggattaatca gcgcaatttt gatcttaacc
      121 taccgcaaaa caaccttgct tcatgataca aatggattct cattatctga acaatacgca
      181 gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg taacacggct
      241 ttgtatccag tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga
      301 gctttcctca cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc
      361 gaacgccata gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa
      421 tcacgatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc
      481 ggagcatcat ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt
      541 acgttaccca agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa
      601 actcaataac ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat
      661 gtatatccgc cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac
      721 cgctttcgcc gccaccacca tttccggatt gcttttgcaa caccccactt tcagcaatac
      781 ccccaccatt gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa
      841 tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc
      901 cagtctgctg acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct
      961 acgcggatgg gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc
     1021 attcctgcgg tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt
     1081 gcgctgcttg gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat
     1141 attgagtggt caacctttat ggcctccatg ctggtcggca ccattggtat tcaatggtcg
     1201 cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg
     1261 ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc
     1321 gaaccgttga tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg
     1381 ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa
     1441 aattgcctcg cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata
     1501 aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt
     1561 tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc
     1621 aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt
     1681 cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag
     1741 cgatgtcgat ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc
     1801 catggggaaa ttcgccatgt atccggactg gcaacccgat gccgacttta tccgcctggc
     1861 ggctctatgg ggcgtggcgc taagagagcc ggtaaccacc gaagaactgg cctcattcat
     1921 tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc
     1981 gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac
     2041 ggtcagtgaa cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt
     2101 ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa
     2161 acgggtgaag agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc
     2221 gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgccca
     2281 ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg
     2341 ttaagcaaag cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt
     2401 tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg
     2461 ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat
     2521 accttcagga atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat
     2581 ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc
     2641 aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt
     2701 aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt
     2761 aacagtttgt gggtgatctt caactgggat agctaccgta gccgtgtaac aggtaaagag
     2821 tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg
     2881 aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg
     2941 gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa
     3001 aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc
     3061 ggaaaccagg ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc
     3121 gcgaagcaaa agctggcttc ggtaaccgat gtcgaaaaca tcaagaacca ggtgctggaa
     3181 aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga cggcattcag
     3241 ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacaactccg
     3301 ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc
     3361 atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct
     3421 gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa
     3481 aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca
     3541 gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc
     3601 ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc
     3661 tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac
     3721 ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg
     3781 cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt
     3841 cagcaaatac attttgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc
     3901 gctgggctgg atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt acagcctgct
     3961 ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga
     4021 actggtgaaa tcccagtcac gcgacggcga cccggacttt gcggcttatt ataaagagcc
     4081 gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga
     4141 gcggacctat tttgataacg aggctttccc ggacctcacg cctgaactgg gcgcgttgaa
     4201 aaatgaaggc ctggatttca gccacacgca gcagctgccg ggaacagatt acacgattgc
     4261 gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc
     4321 cgcttctgtt tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc
     4381 gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt
     4441 cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc
     4501 cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg
     4561 gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt
     4621 cgatacccat cacccggatg gttttatctc tcgtacctgt aaccgcaaaa aatatgattt
     4681 tgacggtaaa ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac
     4741 gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc
     4801 tgaccattta gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa
     4861 cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa
     4921 cacgatggat aacggagcga cagtgctgga cattctcggt ggcgataact atctcggact
     4981 tggtcgtagc agtttatccg ggcagtcaat gtcggaaatc ttcctcaata tcaaagagaa
     5041 aacattggcg tggaagccgg atatcatccg tctgtggaaa ttccctaaag agatgaaaga
     5101 gttcaccatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc
     5161 gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg ccagaaagcg aatactcagc
     5221 accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg
     5281 ttgctacaag atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt
     5341 ctcgcagggg caacttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggaa
     5401 gagcaagacg gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga
     5461 tacgctgaag attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt
     5521 cgccggtgcg ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg
     5581 ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc
     5641 gaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat
     5701 tccggtacgc gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac
     5761 cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc
     5821 tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat
     5881 ggtggaaatt aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata
     5941 gggtgcgacc tacacacctt ctccggctta caacacatta ttaagaaaca aaaaaaacag
     6001 cgcccaacac ttccccggac gctgcatggc aactttaata atctcgcctt tcagaaagta
     6061 attacgcctt taattaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
     6121 gtggcgaacg gatagcgatg ggttaacatc atgccagcag tgatttttcc ttccgccatc
     6181 agtcgaccga ctttagcaaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt
     6241 tcttttttat ggaattccgg atcggagaac tgcaactcac ctttaaacag gccgacaaat
     6301 accaccgtgc cgccgtgacg aatcagattc acggtgttat tcatcgcatg ttgattacct
     6361 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgcag ctgcgcgtca
     6421 aaatcttcgg ctgacgggtc cagcacgggc aactccagac gcgttgccac atgttcacgg
     6481 cgcgccggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     6541 gcgcccagac cgattggccc ggccccgacc accagcacct gctcgccggg agcaatggct
     6601 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgcggc cgcctgcggg
     6661 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     6721 ccatcctgat gcacgccaat gactgaaatt ttttcacagc aattggtacg cccgctttta
     6781 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     6841 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaacc
     6901 cgtggataac taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc
     6961 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     7021 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     7081 ctctccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     7141 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     7201 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     7261 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     7321 tgtacaacat cagccgcact acagtgcgtc atattctcag ccacttacgc gaatgcggcg
     7381 tcttaacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     7441 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     7501 cgatgataaa ccagcgccag ttacgcccag gggaaacttt ttctgaactg caactggctc
     7561 gggccgcggg tgtcagcccg gtggttgtaa gagaatatct tttaaaattc ggccgttata
     7621 atcttattca aagcgaaaag cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     7681 ctgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     7741 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc
     7801 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     7861 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     7921 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     7981 ttgccgtcga tgagcatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     8041 ccacccttgc gctccgtaac catttgaatt cagcgaaaca atcaatgatt cgatccatta
     8101 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg
     8161 cacggtttta tcaaaagata aataccggga aattcccgct tacctatgct cacaatcagt
     8221 cgactaaaaa tgtttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg
     8281 tcaaccttgc gtaatgcaca attacgtaaa caattaaaat tataaataca ggagtccggc
     8341 gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca
     8401 gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa
     8461 accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg
     8521 ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc
     8581 gctttgctct ccgtgttttc actcgcttac ggcattgcgc aacttccttg cggcccacta
     8641 ttggatcgta aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc
     8701 caggccatgt ctggcatggt gcacagcttt acgcagttcg tgttggtgcg tatcggtatg
     8761 gggattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga ctggttcaac
     8821 atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgcc
     8881 gtaagcccac cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt
     8941 accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc
     9001 gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc
     9061 cgccgagatc cgctcagttt tgccgaatgg cgcagcctgt tccgtaaccg cacaatgtgg
     9121 ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt
     9181 cctggttacc tgcaaacagc ctataacctg gatttaaaaa gcacagggtt gatggcggct
     9241 atccctttcc tgtttggtgc tgccgggatg ctggtcaacg gttacgttac tgactggctg
     9301 gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattgc cgggatgttc
     9361 tgttctgccg cctttacgct gatagtacca caagcgacaa catccatgat ggcggttctg
     9421 ctgattggca tggcactgtt ctgtattcac tttgccggaa catcctgctg gggcttgatc
     9481 cacgtcgcag ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc
     9541 ttcatctgcg cctctttcgc gccgatcatt actggtttta ttgttgatac aacccactca
     9601 ttccgtctgg cactaatcat ttgcggttgc gtcaccgcag cgggagcact ggcgtacatc
     9661 ttcctggttc gtcagccgat caacgacccg cgtaaagatt aaactcccta tgcccgataa
     9721 cattttgctt atcgggcatt tttatggcga ttaaaacgtt tcccagttct ccccgctatc
     9781 tgccactgcc attttacgcg gcgtagctgg cgtcacggtt tttaccacag ccgatgtttc
     9841 acgctgctgt tgctgctgtt gctgaatccg gaacactgcc acagcttcgg tcaggcgact
     9901 ggcctgctct tccaacgcgg cggcggcagc ggcagactct tccaccagcg cagcgttctg
     9961 ttgagttacc cggtccatct cagcaaccgc taagccaacc tgatcgatac cacggctctg
    10021 ctcatcagaa gcagaagcta tttcgcccat aatgtccgtc acgcgggtca cagcactgac
    10081 aatctccgcc attgtttccc cggcgctttc gaccagcgta gagccaacat ccactttccc
    10141 caccgagtct tcaatcaggc ttttaatttc acgagccgcc tgggcgctac gctgggccag
    10201 attacgcact tctcccgcaa ccaccgcaaa accacgtcct tgctcacccg cacgcgctgc
    10261 ttcaaccgcc gcgttcaaag ccagaatatt ggtctggaag gcaatgccgt caattacgct
    10321 gataatatcg gcgattttct gcgaactggt ggagatatca cgcatagtct gcaccacgtt
    10381 atctaccact ttgccgccgc gttgcgccgt ttcagaagca cttaacgcca ggtggctggc
    10441 ctggcgcgca ttctcggcgt tctgtttcac cgttgcggtg agttgctcca tgctggctgc
    10501 cgtctcttcc agcgaagcgg cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt
    10561 agcgatttcg ctggcaccgc tatagatggc attggccccg ttgcgcacat caccgacggt
    10621 acgcatcagc tctccctgca tatggcgcaa actctctgcc agttgcccca tctcattaga
    10681 gccatccacc tcaatcggtt tcaccagatc gccgcctgca atatgacgaa tgctgtcaat
    10741 caggcgattc attggcgcta ccagcgaggc tttaataccg aaccagacgg cgaagatgac
    10801 cgccagtacg acgatcatca cgcccaccag aatccacatc gcctggctgt aggaggcatt
    10861 gttatcgctg acggcgatat catagagccg atcgttttgc tccatgtaag ccacatactg
    10921 cttctcgaaa ccgtcctgat atccctgggt cggctgatca aagaactcgt tgattttgcc
    10981 tgcacctaac agttggatca gctccgccag cgcattgtga taaatatcgt aattacgttt
    11041 gatctctgcc gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt cgtaatctgc
    11101 ccagtttttt tccgcctgtt tcaacgaaat actggcactc tgcatcagct cagcaacggt
    11161 tgaaccgcta ccaatattat tctgatccat catgtagcgg atacccgcgc ggttgagggt
    11221 gttacgcgtc tgcaacaacg cgacccagct gccattcagc gtggattgct gctggcgaat
    11281 ggtttgtaaa acagtgaaat tttctttgtc attctttaag gcattaaaga acagaccgcc
    11341 tgatgtcagt tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg tcacgatctt
    11401 gatacgtttt aacatggttt ctctttcctg tggaccgatg caaaattttc ggcctgaaat
    11461 gagaaaactt tataggtagc gacttgaaac tggttaattt tacctcactt tttttaattt
    11521 ttgttcaaaa atacaataac cacttattga tacattttct tttcttattt ttttcacacg
    11581 aattgtatct attttgaacg aaatatttta tagagtgtgg aattttaaga aaggacatct
    11641 gaaatgttaa accgtgactt cgatcatctc tcaacgctat tacatctctt ttttgaacgt
    11701 gaggatattt gccaaaagct taatattatt gaccaaaaca atatcaccgg ttgggaagtg
    11761 tggttccagg tagagtttgc taatatgctc tgctcaacag accatgaatg gtggcgtgaa
    11821 caagcacttt cctgtgatat gcgcaaaaaa cctgagcgcc cgacactacg cacagatttc
    11881 ttactacgta aaaaaggctg ggcacaagat agctatatcg cgcttgaaat aaaacaaaac
    11941 agagacgcga ccagttgtgt taaaaatatg atcgcagact tagaaaaaag cgcaaaaata
    12001 aaacgttccg aactcgattt acgcagtttc tggaccttgg gtatcacccc tatgatagac
    12061 agggaaaggc tggactctct catcgattgc tatttagatg agaaatttta tcttactaaa
    12121 tctcgtaaaa aacacgtttt acttaaagaa ataaataaca caccatattg ctatatt
//
LOCUS       DAZFTN010000131        11999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFTN010000131 DAZFTN010000000
VERSION     DAZFTN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..600
                     /gene="yebB"
                     /locus_tag="ACHKV2_004158"
     CDS             <1..600
                     /gene="yebB"
                     /locus_tag="ACHKV2_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO6941936.1"
                     /translation="NINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGHN
                     GEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRK
                     LYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLG
                     SIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(602..1123)
                     /gene="ruvC"
                     /locus_tag="ACHKV2_004159"
     CDS             complement(602..1123)
                     /gene="ruvC"
                     /locus_tag="ACHKV2_004159"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO6941937.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1158..1898)
                     /gene="yebC"
                     /locus_tag="ACHKV2_004160"
     CDS             complement(1158..1898)
                     /gene="yebC"
                     /locus_tag="ACHKV2_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6941938.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1927..2379)
                     /gene="nudB"
                     /locus_tag="ACHKV2_004161"
     CDS             complement(1927..2379)
                     /gene="nudB"
                     /locus_tag="ACHKV2_004161"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO6941939.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2497..4269)
                     /gene="aspS"
                     /locus_tag="ACHKV2_004162"
     CDS             complement(2497..4269)
                     /gene="aspS"
                     /locus_tag="ACHKV2_004162"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO6941940.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALCVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4579..5145
                     /gene="yecD"
                     /locus_tag="ACHKV2_004163"
     CDS             4579..5145
                     /gene="yecD"
                     /locus_tag="ACHKV2_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6941941.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5142..5960
                     /gene="yecE"
                     /locus_tag="ACHKV2_004164"
     CDS             5142..5960
                     /gene="yecE"
                     /locus_tag="ACHKV2_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO6941942.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6013..6408
                     /gene="yecN"
                     /locus_tag="ACHKV2_004165"
     CDS             6013..6408
                     /gene="yecN"
                     /locus_tag="ACHKV2_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO6941943.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6449..7192
                     /gene="cmoA"
                     /locus_tag="ACHKV2_004166"
     CDS             6449..7192
                     /gene="cmoA"
                     /locus_tag="ACHKV2_004166"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO6941944.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7189..8160
                     /gene="cmoB"
                     /locus_tag="ACHKV2_004167"
     CDS             7189..8160
                     /gene="cmoB"
                     /locus_tag="ACHKV2_004167"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO6941945.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8325..10754)
                     /gene="torZ"
                     /locus_tag="ACHKV2_004168"
     CDS             complement(8325..10754)
                     /gene="torZ"
                     /locus_tag="ACHKV2_004168"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO6941946.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10779..11879)
                     /gene="torY"
                     /locus_tag="ACHKV2_004169"
     CDS             complement(10779..11879)
                     /gene="torY"
                     /locus_tag="ACHKV2_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO6941947.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2741 a         3185 c         2928 g         3145 t
ORIGIN      
        1 aatattaatt accctgctga atatgaaatt ggagataaag tctttacatg tataggtgct
       61 gccttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt cgggatcatt
      121 atcggtcata acggtgaaga cttcctggtt gcagaaagcc gggttcccct ttcaaccatc
      181 accacgctat ctcgttttat taaacgttcg tctaatcaac gctatgctat aaagcgatta
      241 gacgctgggc tgacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta
      301 cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt
      361 gtttttgata tttataaaga agcgctatgt attccggtgg gtgaaataga gacgtttgga
      421 gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcctgggt
      481 tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccaggtttg
      541 gtgttgattc atgcggaggg agtggaaacg cctcagccgg aactgaccga ggcggtataa
      601 cttaacgcag tcgccctctc gccaggttca gccgcgattc gctcatctgc atcgcattct
      661 gactaacgtg gcagtgggtg atggcaatcg ccagcgcatc ggcggcatcc gcctgtggat
      721 tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt tcggcactac
      781 caatacctac caccgtttgc tttacctgac gtgccgcgta ttcaaatact ggcaactcct
      841 gattcaccgc cgccacaatc gccacgccgc gcgcctggcc cagtttcagg gctgagtcag
      901 cgttctttgc cataaagact tgttcaatgg cgaaataatc gggctggaac tgggtgatga
      961 tttccgtcac gcccgcatag atgagtttca gacgagacgg taaatcatcc actttggtgc
     1021 gtatgcatcc gctacccagg taggacagtt gcctacctac ctggcggatg acgccgtagc
     1081 cggtcacgcg cgaacccgga tcaatgccga gaataatagc catcacgcgt ctccgttttg
     1141 ctgtttagca ggcctcatca gagagtcgct gcgacctcat cagagatttc accgttatgg
     1201 taaacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt
     1261 gcggtttctg catccatatc agctttggta gacgggatca tggaaacttc cgcgctgtct
     1321 gctttcagac ctgccgcttc cagagcgtcg cgcactttac ccatttcttc ccatgcagtg
     1381 tagacatcaa tcgcgccgtc atcataggtc acaacgtctt cagcacctgc ttccagtgct
     1441 gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tgacgccttt tttgctgaac
     1501 agataggcta cggaaccatc agtaccgagg ttaccgccac atttgctgaa tgcatgacgc
     1561 acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg
     1621 ccaggaccgt aaccttcgta gatgatggtt tccatgttcg catcatcatc accgcccaca
     1681 ccacgtgcaa ttgcgcggtt cagagtgtca cgggtcatgt tgttagacag tgctttatca
     1741 attgctgcac gcagacgcgg gttagcgtcc ggatcgccac cgcccagctt agccgcggtt
     1801 accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta
     1861 cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc agatagccct
     1921 gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt
     1981 agtgagcgcc gccgcagcag gcgcatcaag ccacttgtaa gccagatgtt cagtgaaaac
     2041 gatctgccgc tcatgcggaa gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc
     2101 cggcgcatag cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc gctgacagtc
     2161 aattaaggtc agttgttcag cgacaacatc aatggtgacc tcttccttta cttcgcgcat
     2221 ggcagcttgc ggcgcggttt caccctcttc cacgctgccg gttaccgact gccagaaatc
     2281 ggggtcatca cgccgctgca acatcagcac ccgtttcgta tcttgtgcgt agatgaccac
     2341 taagatcgaa acgggacgct tatacacttt atccttcacg ctgcctcttc gttgactgcc
     2401 ttcgctcatc ccattcacat agttatctat gctcatggga gttcactcag ttgccgcctc
     2461 gatgcaacgc gaatgatttc gtgtatttga gtcatatcag ttattctcag ccttcttcac
     2521 aacctgaatg ctcagctcag ccagtgcagc cgggttagca aagctcggtg cttcagtcat
     2581 cagacacgct gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc
     2641 ggtcagcagc atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc
     2701 gtatttcaga gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc
     2761 cagaatacca aacaccgtct gctgcatatc accattatgg atacgtactg aaccaccgcc
     2821 cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt ccggtgcagc
     2881 tttcagctct gcagccgtca tgtctttcgg tgaggtgaac ggatggtgca ttgccgtcag
     2941 gccgccttca ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt
     3001 gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc ccatcgcgtc
     3061 ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt
     3121 acgatccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat
     3181 accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta
     3241 gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg cgccacccgg
     3301 aacacacaga gcggcaacgc gacctttcgg atcgttcgcc ggacctgcaa atactgcaaa
     3361 ctcaacagat ctcagcagat cagcaacgtc ggtcagttcc atcgggttac gcagatccgg
     3421 tttatcagaa ccataacggc gttcggcttc cgcaaaggtc attactggga aatcgcccag
     3481 atccacgccc ttcacttcca gccacagatg gcgcaccagc gcttccatca cttcacgcac
     3541 ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc
     3601 agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtagcggt caaagccgga
     3661 catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt
     3721 gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat
     3781 cggagtttcg atgtcgagga aaccgtggtc atccataaaa cggcgcacca ggctggtgat
     3841 tttagcgcgg gttttcaggc gctgagccat ttccggacga cgcaggtcga ggtagcggta
     3901 tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcgcg
     3961 gttgatgata gtcagcgagg acgccagcac ttcgatttcg ccggtcgcca tatcgcggtt
     4021 aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg
     4081 cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat
     4141 accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac
     4201 ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc
     4261 tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga
     4321 ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg
     4381 aaattctgcc gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat
     4441 cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga actcatacaa
     4501 attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgtgag gcacgatttt
     4561 gttttttcag gagtcatcat gcttgaactt aatgctaaaa ccactgcgct ggtggtgatc
     4621 gatttacaag aaggcatctt accttttgcc ggtggtccac atactgccga tgaggtagtt
     4681 aatcgcgccg ggaagctggc ggcgaaattt cgcgccagcg gtcagcccgt gtttctggtg
     4741 cgcgttggct ggtctgccga ttacgccgaa gcattaaaac agccggttga tgccccttcc
     4801 cccgctaaag tgttgcccga aaactggtgg caacatccag ctgcattagg tgcaactgac
     4861 agtgatatcg aaataatcaa acgtcagtgg ggtgcgtttt acggtacgga tctggagttg
     4921 caattacgcc gccgaggtat cgatacaata gtgttatgtg ggatctcgac caatatcgga
     4981 gttgaatcca ccgcccgcaa tgcctgggaa cttggcttta atctggtgat tgccgaagat
     5041 gcctgtagtg ccgctagcgc cgagcagcac aataacagta tcaaccatat ctacccgcgc
     5101 atcgcccgtg tgcgtagcgt ggaagagatc ctcaacgcgt tatgatttac atcggtctac
     5161 cgcaatggtc gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc
     5221 gtcactttaa ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg
     5281 tcctgcgctg gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga
     5341 ccatttcgca tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc
     5401 gcatgtcacc gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg
     5461 gcccacggga gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt
     5521 atggtgtgga agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta
     5581 atcgcggttt acatcagcgc ggcgttaatc aggtgatttt agacagccgc ccggttcatg
     5641 cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg
     5701 tacatgctgt actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga
     5761 cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca
     5821 ctacgcctta tcttttttta catacgccag atattgccca ggccccggaa ctggtacata
     5881 ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac
     5941 agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta
     6001 aagggagttt gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag
     6061 ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt
     6121 tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc
     6181 gcgattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt
     6241 tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg
     6301 ttccgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg
     6361 gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc
     6421 cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc
     6481 tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat
     6541 gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga
     6601 gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac
     6661 gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc
     6721 cccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt
     6781 agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct
     6841 gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcgttactgg ataaaattta
     6901 tcaagggctg aaccccggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc
     6961 caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag
     7021 cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt
     7081 ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca
     7141 gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt
     7201 ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc
     7261 gcgcagattg ctaactggca gcgcgagcag cagcacgggt tgtttaagca gtggtcaaat
     7321 gcggtggagt ttctacctga aattaaaccg tatcgtcttg atttattgca tagcgttacg
     7381 gcagaaagcg aagagccact gagcgcaggg caaattaaac gcattgaaac gctgatgcgc
     7441 aacctgatgc cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa
     7501 tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc
     7561 accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc
     7621 gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg
     7681 cgtaaactac tgggtaatga tcagcgcgcg catttgttac cgttaggtat tgaacaactt
     7741 ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttatca tcgtcgttca
     7801 ccgctggagc atctctggca gttgaaagat caactggtca atgaaggtga actggtgctt
     7861 gaaacgctgg ttattgatgg cgatgagaac acggtgctgg tgccgggcga tcgttacgcg
     7921 caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag
     7981 tgtggttttg tagatatccg cattgcggat gtgagcgtta ccactacaga agagcagcga
     8041 cgcactgaat ggatggttac cgagtctctg tccgatttcc tcgacccaca tgatccgagt
     8101 aagacagttg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa
     8161 aggtctggta ttactgccgg atgcggcgtg aacgccttat ccagcctaca aaaccttgct
     8221 aattcaatat attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagcttta
     8281 tgtttgcata acctcagcgc ccgtttccgg gcgcttttca cgtcttatgc ctgaacagct
     8341 ggctgatcaa acgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc
     8401 gcagagttac cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg
     8461 ttcgcactgc cgtttttaca caagccattt tccagatctg gccatgcacc ttcatgcacg
     8521 cataccacgc cttttttgat cccgtcagtc accacagcgc ctgtcaaaat ctgaccacgt
     8581 ttgttccaga cacggaccag atcgccattc gcaataccaa agcgagcagc atcttcggtg
     8641 tgaatagtga ttggttcacg atcggcaacc gcatattttt tacgcagttg cgcatagtta
     8701 agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgtaactg cttctcgtcg
     8761 gcggtacctt tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag
     8821 ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca
     8881 tttttcaccg gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg
     8941 cggcgcattt cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg
     9001 cgttgcgcac gggcaccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca
     9061 tctttacctt cggtatagat ctcttttccg cctggtttca gtaattccgc aagttcggca
     9121 aacacgtcaa aatcgtttcg cgcttcaaat tgcggagcga cagcctgctt catcggtaca
     9181 atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg
     9241 atcggtaata cgatatctgc atgtttagca gctgcggtcc agtagcattc agaaacgacg
     9301 atcatctccg gtttctgcca tgctttgatc agacgattag tatcctggtg atgggtaaag
     9361 ttaccgccac ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta
     9421 tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc
     9481 gtcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc
     9541 ggcagcacgc cgccaacacg cgtcgggttg ccaccgttgg agtaatgata agagaaacca
     9601 aaaccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag
     9661 tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag cattgtgcga
     9721 ttcgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc
     9781 gccgtccagg ccgcgctctt cggagtatta tcgctcttac ctgtcagata ctcttcaaac
     9841 tgcggatacc cggtggtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc
     9901 agggtatgcg caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag
     9961 gtggcattat agccaaagaa ctcgatggtt tcagagcgga taggatcaat ggcaatcact
    10021 ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc
    10081 caggcaattt ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt
    10141 tccagaatca gcggccagga ggtctgctgt tcatacacct ctacagaacc gaccacatgc
    10201 ggcatgatca cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc
    10261 gccaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc
    10321 cagccgtaag atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt
    10381 tgctcatgaa tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca
    10441 cgacctttcg ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga
    10501 atacgcgccg tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt
    10561 ttcgccagcg cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gttcatcgct
    10621 ccccaacgtc ccgcggtgag gattttaccg cccttctctt ctgcccatgc gggtagcggt
    10681 gctgccgatg tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt
    10741 cttgttaatg tcataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct
    10801 ttggcgttgt actggaaata ccgcgttaaa atgtccagtt cgttttcgct catgctggtt
    10861 cgtgccccca ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca
    10921 ataggggcat gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc
    10981 agtttacggt ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat
    11041 tgattgccgt attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct
    11101 tcacttaatg tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg
    11161 ccctgcattt gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg
    11221 gtgagatcgg taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga
    11281 taaatatgtg cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg
    11341 ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag
    11401 gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca
    11461 atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat
    11521 tctttccata ctgtttcggc catttcctgg cgatgagctt cgaacttatc gtcactgtct
    11581 attttgccgc taacaaattc atgataaata tctttagatg cttttaattt agcgaataaa
    11641 taatccatcc ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg
    11701 ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa
    11761 aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccatcaa gaacccgcca
    11821 ccaccaacca caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata
    11881 gattcctcta attaaattcc agaacaccga aatcattgta taaataaata cacaacgaat
    11941 tgactacttg cttaattcgg cacaacactg cctgaaataa tcgctaaaga atatgattt
//
LOCUS       DAZFTN010000132        11994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFTN010000132 DAZFTN010000000
VERSION     DAZFTN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..200)
                     /locus_tag="ACHKV2_004170"
     CDS             complement(<1..200)
                     /locus_tag="ACHKV2_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6941948.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASYSAAIFLIPICISFDT"
     gene            complement(209..595)
                     /locus_tag="ACHKV2_004171"
     CDS             complement(209..595)
                     /locus_tag="ACHKV2_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6941949.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(656..1399)
                     /locus_tag="ACHKV2_004172"
     CDS             complement(656..1399)
                     /locus_tag="ACHKV2_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6941950.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(1410..1811)
                     /locus_tag="ACHKV2_004173"
     CDS             complement(1410..1811)
                     /locus_tag="ACHKV2_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6941951.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(1808..2386)
                     /locus_tag="ACHKV2_004174"
     CDS             complement(1808..2386)
                     /locus_tag="ACHKV2_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6941952.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(2398..2673)
                     /locus_tag="ACHKV2_004175"
     CDS             complement(2398..2673)
                     /locus_tag="ACHKV2_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6941953.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(2666..2989)
                     /locus_tag="ACHKV2_004176"
     CDS             complement(2666..2989)
                     /locus_tag="ACHKV2_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO6941954.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(3076..5103)
                     /locus_tag="ACHKV2_004177"
     CDS             complement(3076..5103)
                     /locus_tag="ACHKV2_004177"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6941955.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKNERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(5048..5383)
                     /locus_tag="ACHKV2_004178"
     CDS             complement(5048..5383)
                     /locus_tag="ACHKV2_004178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6941956.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(5505..6629)
                     /locus_tag="ACHKV2_004179"
     CDS             complement(5505..6629)
                     /locus_tag="ACHKV2_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6941957.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(6557..6769)
                     /locus_tag="ACHKV2_004180"
     CDS             complement(6557..6769)
                     /locus_tag="ACHKV2_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941958.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(6766..8868)
                     /locus_tag="ACHKV2_004181"
     CDS             complement(6766..8868)
                     /locus_tag="ACHKV2_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6941959.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(8868..9359)
                     /locus_tag="ACHKV2_004182"
     CDS             complement(8868..9359)
                     /locus_tag="ACHKV2_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6941960.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(10034..10186)
                     /locus_tag="ACHKV2_004183"
     CDS             complement(10034..10186)
                     /locus_tag="ACHKV2_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941961.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(10174..10641)
                     /locus_tag="ACHKV2_004184"
     CDS             complement(10174..10641)
                     /locus_tag="ACHKV2_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6941962.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(10638..11135)
                     /gene="rrrD"
                     /locus_tag="ACHKV2_004185"
     CDS             complement(10638..11135)
                     /gene="rrrD"
                     /locus_tag="ACHKV2_004185"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO6941963.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(11135..11350)
                     /locus_tag="ACHKV2_004186"
     CDS             complement(11135..11350)
                     /locus_tag="ACHKV2_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6941964.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            11493..11891
                     /locus_tag="ACHKV2_004187"
     CDS             11493..11891
                     /locus_tag="ACHKV2_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941965.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            11906..11994
                     /locus_tag="ACHKV2_004188"
                     /pseudo
     CDS             11906..11994
                     /locus_tag="ACHKV2_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2819 a         3496 c         2785 g         2894 t
ORIGIN      
        1 gtatcaaatg aaatgcaaat cggtatcaaa aaaatcgccg cgctatacga ggcaaactgc
       61 gcatccatcc acacatcact gaagctctgc atccggaaat aatcgcccca ctcaccaagc
      121 tcagtggccg acatttccga cagcatccgc cgccagtctg cccgccggaa ctcccgggca
      181 agccgcatga caaactgcat ttcccgcgtc aggacttttc cggcgtcagc acctcatgct
      241 ccaaatcccc ggcattctca atggctccca taccgctcag cgacagaacc atctccgccc
      301 ccgctcccag ggcatcatac gaccatgttg taataacgga tgcgcaaagc gtctcaacat
      361 cctgagactg ttccgcattc cacagtgagc gggaaaccag ccaggcattg atatccatcc
      421 ccatccgcag aaaagcaatc tgtcgttcag cctccggcag ttctccctct tcggcatcaa
      481 actttgccgt tcgctgctga acaaacgcca gatattcaat tctctgcagc ccggacagct
      541 cactgagcac cacggactgc ttttcataat taaacgtgcc ctgtttcaga aacatcatgt
      601 tctccacctg caaaaaagcc ccggataacc ggggcaaatg atgagtatcg tcctgttaac
      661 ctgcagcgct gacagtcacc gcagccacag ccacaaaatt cccgtcagcg gtcatgccca
      721 caatgctgac gctgccctgc ttcacgcctt tcaccgtggc cacaagtccg ttcagggtca
      781 cagtggctgt ctgtggatct gtcgaatgca cactgatcgc tttgtcactg gctccgtcag
      841 gttttactgt aaaggtcagc gtggtggttg ctcccacatt gacactggcg gatgccggtg
      901 ccaccgtcag cccggtaacg cccacagttt cagtgccctc ctctgccaga tatggacgcc
      961 ccacaccgct gattttcact gtgcgggtca tcacgtcttt tgaggcaatg gttttaccca
     1021 gtgagctcag ccagccgcgg aaaacatcaa cagtgccgtt gggatatttg atacgaaacg
     1081 cgcagacttc accggagtcg aacaactgaa ccagtttttt ctgcccgctg tcacccggac
     1141 gccaggccag cgtcgccgaa gtatcaccaa cggatttctg cccctgggtt gtcgttttcc
     1201 agtctgcatc ttcatcatcg agataagtgt catcttctgc atcagcggtc atttcgccag
     1261 gttgcagatc cttcaccatc gcaagacgca gccagtcagt gtccgacaaa gggttcgcaa
     1321 acgcatcgcc gttgccggta tacatccaga acgtcgtccc cgcacctttc gtctttgcca
     1381 gtggatttgg cgtggtcatt accacctcct taattcgtgt acgtgatctg gtacgtgatt
     1441 tccgccatcg cccaggtggc catctcatta tcacgttgat agttaaaacc gagtgggatc
     1501 agggtgtcga tgagtccgga aagtgccgat acatcattca gggccgggaa aatggtgctc
     1561 tccatccaca tatccagctc tgaatccggt gcctgtgccc ggatgaagac agcaatatgc
     1621 agaacagcct gccagtcatc ttcatccgtc atttttccgg tgtactgagc atcactcagc
     1681 cacaccgcca cggcaggcag ttcctgcgca tcaacaaatg ccggaagccc gtcaaaaaac
     1741 gtggcgctgt ctccacactg ttcccgaagg cgtgccagta cgacctggcg gatttgtgta
     1801 tgtcggttca tcgggtcagc cataacctca gttgttgttt cagtgcatac cccagctgtt
     1861 tcggcatttc cgcagcaatg atgcggtcgc gggcatcttc aaatgcctgt gtcagcggtc
     1921 cggacagcgg gattttcacc acatcaatgg ggtaacgatt tttgccatca atacgccgca
     1981 tcacatgcca gcgaccattc gccagttgct gaataaacgc atcccggaaa agatatttac
     2041 ccaccttcag cacactgcca cggtactgca gttttccacc acgctgggcc agtctgaccc
     2101 gggctgtccc cagcttaatg gcgggcagat tgccccggtt aacgcggatc ctggccgtca
     2161 tttttcctga cggactggct ttaaacaccc ggacacgctg acgaaccagt ttcaggggga
     2221 tccctttcac ctggttatct cccgcaacgg tattcccggc aacctgccgg gtggcaaccg
     2281 agcccgcttt ctgtgccaca cggtttatcg cccatgcgct ggcctgtggc accatacggg
     2341 tatcaaggct gttcagattg cggatggcat tctcaagccc cttcatccca cacctcttta
     2401 ctcaataaag atcattggct taccgttaaa gcgttcatgc cgtgtgaccg tccattgttg
     2461 tccgtcataa acaacgcgat ccccgcgccg tgggcggtat cccgaagaaa acaccaccag
     2521 agagaccgca ggtccggaca gagcattcag ctctgccagt gtttctcccg ggatcacagt
     2581 catatcgaca tcattaatcg aggctgtctt tcccatcttt ctgaccgtga tagcatccat
     2641 acgcgctgcc agccgggaaa agggatcaga cattgagttt taccggcact tcttctgcac
     2701 tggttccggc atctgcccag acaaccccga ccagcggatc agagccgctg ttagtcagct
     2761 gaacttttcc tgacttcaga taaaccttct tacccgtttt catgtcatcc gttttcagct
     2821 taggcagcat aaacacacct tcggtcatgc cgtcgcctgt ttcaccctgt ggaatatcgg
     2881 tcagcgccac cgcaaaaaca tcgcccacct gcaccagatc tccgctgctg atggctgcac
     2941 tggcaacaat cgccaccgtt tttccttctt ctacaaaatt ctttgccata actgtctccg
     3001 cacagccccg ttcaggggct gatttcaggt acaaaaaaag cccttacggg ccatcagagt
     3061 tgttgtctgc gacgtttacg ccgtacattt caccagaccg cggtgatcaa ctggcgcgac
     3121 accggcgtca atacgcactt tcgttgtcac gccatccaca ctgaagccct ccatctgatc
     3181 aatatatggc gtatccacac cgttgagata agccacttca atcgtatcgg agccttttga
     3241 cgcagccagg tagaaggtgg tctggctgtt atcatcaaga cgaggctctg caataacggt
     3301 cgcaaaatct ttcaccgggt taataatacc ggcgttaatg tcagccccct tgacacttga
     3361 ggagcgaatg acctggttag caacagactc catcgccgtc ggtaccagta cgaacgcagg
     3421 acgaatattc agatgacgct ccccctcttt ctgaacgcgc atcaactggc gggctttatc
     3481 cagcgatgcc acgtccattg cagcgctctc cagtacgttt gcatgtttcg ctttatcgaa
     3541 cagacttaca ttatctgtgg agattttcgg gttagacgtc agaatggcat aaaccagatc
     3601 ggcaatagtg gatttcgccg cacggcccag tttcatcggg acatcggtca gcatattcag
     3661 atcatcattg ataatggcct gacgggtgat actgaacagc tcgccatagg tcgccagtgc
     3721 aatagtggcc tgtttatctc cggtggtgac gtatttatat tccgcccctt cacgcacctg
     3781 acgcagagca ctgaagcccc ccatacccac acgatgggca attttaaaat cagacaactg
     3841 acctttccgc gtccactgtt cataggtttc aggggcatct tcccagccct gcagaatggc
     3901 tttgttcgca acatccagca gaatattacc gaagtcagac gtactgtgtg tgaacgccgc
     3961 accgaccatc tgcatcgggt tataactgga aaccccaata ccccgttcag tcagtgacat
     4021 acgggcatat tcacgcaggg tcatcccgtt gtagacatta tcacgttcgt ttttttcaaa
     4081 tccggcacgc gccatcagcg cctggcggat cccatccccc acaaaattac cgtttccggc
     4141 ataaatatga gccggggtat ttttattaga tggcgtggac tcgcgcccca tctcgttcaa
     4201 cagcttttcg cgggcctgct ccagcgaaca ttcaggatcg gcaagacact gagcctgcag
     4261 cgtctgataa cgcccgccaa acatggcaaa cagatcatta ataccgttta cacgcgcttt
     4321 ttgctctgcc agtacctgcg cacggatact gttttcatcc accacaggtg ctgctgcctg
     4381 cactggcgtc cgggaggctg caggtccatc atcctgtacg cgtggagcac tgttgcgtgg
     4441 cggagtaatc atgtttcgaa tggattccgg catcttttta aattcctctg tacgttttga
     4501 ctgaatacat gccattgcct taacggctgg cgtcacctga tcagcaaatc catgtgccag
     4561 acattcggca ccggacatcc aggtctcatc cgccagcatg gcagcaattt catcggtggt
     4621 tttcccggtt ttctgtgcat aagcgggtaa cagaaccgcc tcaactttat cgagcaggtc
     4681 ggcataggtg cgcatgtcct ccgcatcacc gcccgtaaag ccaaatggtt tatgaatcat
     4741 catgaaggag ttttccggca taatgaccgg ttttcccacc atcgcaatga ccgacgccat
     4801 tgacgccgcc acaccgtcga cataaacggt aatggacgca ccatgtgttt tcagtgcatt
     4861 aaaaatggcg atgccttcaa agacatcgcc acccggtgaa ttgatattga gattaatgtg
     4921 ggtgatatcg cccagtgcat tcagatcact gataaactgc ttcgctgtaa caccccagaa
     4981 accaatctcg tcataaatat aaatatccgc gtcactctgg tgaccagcct gcatcctgaa
     5041 ccaggaatta ttcttcggac tggtcgtcgg tgtgctgcgg ctcctgtcgt ttcgttgcgg
     5101 cactgctgcc tcctttatca ctggccggat cggtatcaaa taccagatcc agcttgcggt
     5161 tttcatcaat ttcggccttg cgccgacgtt tgacatcatc cggattacga ccacctgcac
     5221 gtacccagtc tgattctgtc gctgctccac cacgaatctg aattttccag gcctcagcct
     5281 ccttaacagg gtcaatccac ggcatcaccg gtccggaata caccgcggta tacagtgaag
     5341 aacggtcaag atcgcggggt agcctgataa caccggatgc cacagcctgt ttcagccagg
     5401 cacgatacat cgggcgggtg acggcaccaa taaaccagtc ctgcaggatc aggtagccat
     5461 cagtggattc aaccagctcc tgacgctggg cgctgtaagt gccgttatag ttgcgcgctg
     5521 tactggaaaa aattcagacg actgcccgcc gccacggcac gcaactgacc attacgaaaa
     5581 gtttcaaggt taggattggg gcgatccgac ttcaccattc cgatttcttc gccgggtttc
     5641 agatcgtcgt aaataatgcc tggctgaatg gtaagctcgc gttccttatc cttgctgcca
     5701 ttaccatccg gttcatagct ctgcccatcg cctttccgga tgtacatccc cagagcagcg
     5761 gcgatccttg ctgcagtcag ctcagaatcc tcatactctt tcagggcact gaggcggatc
     5821 agcacaccgg acaacaaaga cgtcccgcgc atctggtgca gacggcgaac aaatttaaga
     5881 tgcagcattc gctctgcatc cacttctttg gtttccatct gccgtccgga tacgggacgg
     5941 cttttataca ccagatattt ttcgggacgc ccccagtcat caacaaacac gccctgattc
     6001 agcctgttgc tctcatcact ggtcatggga ataaagtctg gctcgagcgc ctccagccag
     6061 aaatgaacac cggcagaagg cgtcaggctg tttatgcgcc cggaaaccat ctgggcaaac
     6121 acctcaccat cgcgcagcca ggtacgcagc atcagacgtt ccagcatcgg acgggtaaac
     6181 tgcccggtga cttccggact gacagaccat tcactccatc gggtgcgtat ctccgctgcc
     6241 agatcacggg caatggcccc attgcgtaat accggatgtg gctcgacaat aatcccgttt
     6301 ttccccacca cccgttcttc cagcttgtca aatacaccaa taaccagatc gtggttgtta
     6361 tcaaggtaac gggcctgctc acgtaacgac acggccccgt actggcttaa ctggtcggca
     6421 gttcggtttt cccgccgggc tttgtgtgtc cgcgtcgttt ttacggcctc ataagcctgg
     6481 atcaccgcac gggaacgcag ccttgccgct ttccatcctg gtgaaaaaac gccaatcaca
     6541 tcatcaagaa ttgccatcag aacctcgcca gccggtaccc gggatgcccc cgtcgtcgtg
     6601 taatcagagc cgcaaggcgg cgctcccact cctgccgtcc ctgccggatc tcagataagt
     6661 tttccatggt catctgctga ccattaaagg tgacggattt tccgtccagc accgccattt
     6721 cagcttccgt ataacgctga atcatggctt cgatatcatt ctggttcata accatcctcc
     6781 ggaagtcagc caggggttaa catcgtcagt tactgttttc ttccgttttt gttttttaac
     6841 aggcgtggat accggttccg gtgagggtga cggttcggta ctgtccggga cactctccag
     6901 ccaggtttcc cggctcgccc accccggtgc atccggccag cggatctttt cgtatccatg
     6961 cagaatgacc agagcctcgg catacaccat caggtcaaaa gcttcgttgg caccgcgacc
     7021 cggcttactc catttcccgt cactactccg ctcttcatac gtcagttcgt cgtaaaacca
     7081 gctccccagc cagtcaggga aatgcacata gccgggacct ggagagtcac gccataacgc
     7141 gttattcacc cggtctttca gtgcatccgt ctgaagaagc cagagcggca catcacctgc
     7201 ggcctgcgcc cgtcggcccg ttcgtccggt gttatcaggg aatgtacggg tgatcagttt
     7261 tgcgcgccgg atgctgtcgc ccttaaacag gtaaatacgt ttaccaaggc catcacgacg
     7321 gcaacgacgc cagaatttat aggcattatc agtgaccccg tcttcaccgc cggagtccac
     7381 cgccattgcc atcagtcgca tttgttgaga aggatcggag gccagcggcc agcttttatg
     7441 aaaaacatcc gtcagcagga catcccagtc ttccggatag ctggccggat caattcgctg
     7501 gctctcaccg tcgctgtcac cgcgcaatga ctgcgtgatg ttgtaacgat caataatcca
     7561 gcgttcgcca cggctgccat agcccgttac ctgaaccaca aaacggcgat gacgtcccgc
     7621 ctgcacatcc actgtcgcca caaggaaatt aacgccatcc ggcacactgc tggaaggaac
     7681 tggctctgcc cgctgctcaa gcagttcact ttttcgttgc tccatgctgg cgcggggaag
     7741 ataaggtaat ccccagtcgg tattgataac cgtcttgagt gtttcttcac ttccggttgt
     7801 ctcgtattcc tgttctgcag taagcagttt gtaaacgagt tgcgagagtg tctggtaagc
     7861 agctgccgga ccctccatcc agaatgacgc aatacgtgag cgtcggggat caccataacg
     7921 actaccatcc gcattgatgg attcaccatc ccgcaaccag accccacgtc cgttcagctc
     7981 acgtttttgt tcaggcataa tccgtcctga acaggaagga cactgaatat aagccgcctc
     8041 acttgccagc acgggatcgg caatatcacg gaaaccagca accacatcgc cgcagggctg
     8101 aaaatactca ccacagtgtg gacagggcca gtaccagcga cggcgatcgc cacggttata
     8161 gagcgacagt atccccgtgg ttggtggagc ctcatgcggt gaagtccgtc gccatttcac
     8221 atccttcaca tccctgccgg gggaactctc caccagcgtc ataccactgg acataaatgt
     8281 ggtggtacgt tttgaggcaa gagagaaggc atccccctcg ccatcaatat cttccggaaa
     8341 acggtcataa tccgtcagcg ccacgcattt ataatctgat gaggacatga tattgactga
     8401 cggccagccg attttcaggt agttgccagc aaggaatgtt ctgtcataaa cgttgttgtc
     8461 atttttgttc ggactcaggc gactgaccac ttccgggctg acgcgaaacg ttctggcgag
     8521 tcgttttttg gagtgttcgc gggctttttc ctccgtcatc tgaatgatca gcatatccgc
     8581 aggatcgcaa atcacgttgt aaatcaccca gccgtcaatc aggccgatag tcttgccggt
     8641 tcgtgccggg ccaacaaata tcactgcgtc gtattcacgc gaggccaggc agttcatcgg
     8701 ctcaataaca tacggtgcca ccagcggatc ccacgggact gagttcccgg cccccatggg
     8761 cacccgcata tactgagcaa cggcatcagc aacccgcatt cgtctcggtg cgcgaaggat
     8821 ataacctgaa tcggttcgtg ctgcctttgc ggtttcctga ttcagcatta ctcctcctgc
     8881 tgtaattcct cctcatcatc cgcacctgct tcggtcaccc gcagggctat ctgatcgcgc
     8941 agatcatcaa taatggactg aacacggctc acagcggcag gctgcagacc acagtcacgt
     9001 tccagaatat ccggtaatgt ctccagcacc tgcacgaccg cttttgccca gatggcaaac
     9061 tcccgtctga catcactggc cggaatgagt tgtgccgttt cctgttcgaa cttaagacgc
     9121 tcacgttcag actgatacca ggctttgcgt tcatgtggat ccatttcgcc ctcagcaacc
     9181 ggcggtggta accccataaa ttcagtcaga atatcggtca accggtatag cttgagtttg
     9241 tcatgtccac cagcgggacg aatgtttttc agtcttgcca cgacagtctg gcggtgcaga
     9301 ccagataaag ccgccagttg attaatattc agcaccaggt ttttcaactc atgatccata
     9361 tttcctccgg agagctttaa acatgcatcg tgcgaacaac tttaagaaaa cgcgttcgac
     9421 gtcgaacaaa aaacactcaa ttcgacatac aaaaaacaaa taaccattaa taatcaataa
     9481 gatgcaaaga tgatggtggc cgataaaaat gcaaaaacta gcctttttcc gcgacgctcc
     9541 cgccccgtgg caggccaccc caccgggagg agccgtcagc ctgaaagcca tgacgaacgt
     9601 ctgatacagc ccttgcatga atggcatcgg gataatccag aaaggaatag catcgaccca
     9661 caagaatctg tgtgagtgtc ctgtttcttc cacccccgca caggactggc gagcatgagg
     9721 gacaaacccg cgaatcatta gcgcggtaaa aacccggtgt gcatcgtttt tgattattcc
     9781 cgcacactcg cgcagaagga gttccccgtc gggctacggt catggttaat gcgggaatac
     9841 agcgacgata cagcgcatga tgtgtcaggc ttgaatacct ttatccgtta aaagggatat
     9901 cagttaagtt atcccgtgta gggtataagc cattatcaaa gccactctgt agggagtggc
     9961 ttttgtaatg gcaataaaaa agccccgcga atgcgaggct aaatccaggt atttttaatg
    10021 actggctctt atctcaacgc agccccttac cgcgcgccag atgctcaata tcaagcatca
    10081 gcaatgagat gtttaaccat gattcactcc agaagtgagc accaccctgt ctacagagcc
    10141 agatgtgaag gatgatgagt aaaattatcg ctatcatcga aggcattgcg tcctgatgta
    10201 ttcctgaagc gttctcagtg ctgtttggtc gcggataatt ccgtcccgga taccgagaac
    10261 gtttcgtcca gcaactggag agagttcgac ggtggcatca ttgcccatgc cggaggcgct
    10321 ggaggtttcg tctgaggatg gcacagggca ttttcctttg acgaacaccc gaccaccatt
    10381 atcaagcttg cgccgaagag catcattttt agctttcgca tcagctaact ccttcgtgta
    10441 tttagcatcg agtgcatcag cagcacgctg gcgctgctgc atgtcagtaa tggtggcggt
    10501 cgcctgcttc agctcactga cttttttatc acgctgttct ttataggcga tggcgttatc
    10561 acggtaatga ttaaccgccc atgactggca gacgatgatg cagataacca gagcggagat
    10621 aatcgcggtt actctgctca ttgttgcccc cacaaacaga cttcacgctc aatctcgcgg
    10681 cgagtcatca gccctttcca ttgcttaccg ccagcgtatg tccagcgccg tagctgatca
    10741 catgcgcctt tgatatcacc ctggtttatt ttgcgaagaa gcgtcgatgt tctgaaatta
    10801 ccagcgccca cgttgtaaac gaacgagtaa agagcgccgc gcgttgtttc cggtatatcg
    10861 actttgatgt acgggttaat ttgtctggcg accgtggcaa ggtctttatt caggagggct
    10921 ttgcattctg cttcggtata cgttttaccg ggaatgatgt cttttccggt gtgtccgtga
    10981 catacagtcc atacgccaac gatatcttcg tatggtatgt agctgacacc ttccaggcca
    11041 tcgtcaccac tcggaccagt gatgagcaca gacgctatgg cagcagcccc accaccaata
    11101 gcagcagcaa cagccttgcg taatgatggc gacattattc acctctcgca gccttacgct
    11161 tatcttcttt aatcttgaaa taaaggtttg tcagatacgt cagcaagcca aacaccaagc
    11221 tacccaatac gcctattgcc acccactggg atggggagac tttgtccagc aactgcagta
    11281 gccagtatcc cgtccccacc gctgacgtgg tgtatgacac acctgttgtt attttttcca
    11341 tctggtacat accccgtctc ccgttatccg gaagctgaca acaataaaaa gccaccagtt
    11401 aattcctgat ggccctgatg cataaacgtc ataatacctg actgttatga ttgacaataa
    11461 tgataatgtt tatatagaaa ggttcccgat gtgtgttaca tatcatttct ccacggggaa
    11521 tatccccacg ccagcgcaga ctcttttacc cgttctcttc tgcgctggct cttttttatt
    11581 atgctgctgc atttacctct ggcaccatgc tttctatctc aacacaatac gtggtacttc
    11641 ttgtaaccaa tatcataacg attaatcgac atagaatttc tcccgtgtac aggaacagag
    11701 ttaaaaagcc ggaaccggaa tcaaatcaca ggatgaccat ctgccagtgg caggtcataa
    11761 aaaaaaggcc gcgccatgcg cagccagaac tcacaaggaa aatgatagaa ggaaataaca
    11821 ttagtgatgt acgcatggcg cctcccgcta agttctgcaa tgatcaaaca gaactcgcta
    11881 cgtgccctta aaactcgatc atttagcccc tccaaggagg attcaccatg cggttgattt
    11941 tttaataaac agtaaacaaa aaagtcaaga attattcatt ctgttctttc atca
//
LOCUS       DAZFTN010000133        11915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFTN010000133 DAZFTN010000000
VERSION     DAZFTN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..632
                     /locus_tag="ACHKV2_004189"
     CDS             <1..632
                     /locus_tag="ACHKV2_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941966.1"
                     /translation="ARAGEYKNYSRDEIKYWRYTSYKGGKLPEGFTDEKFSSAIYNGR
                     IFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGACPSTYELYEGD
                     ATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGDGCKANTCGWDF
                     IVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
     gene            648..1058
                     /locus_tag="ACHKV2_004190"
     CDS             648..1058
                     /locus_tag="ACHKV2_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6941967.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1279..2100
                     /locus_tag="ACHKV2_004191"
     CDS             1279..2100
                     /locus_tag="ACHKV2_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6941968.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2182..2661
                     /locus_tag="ACHKV2_004192"
     CDS             2182..2661
                     /locus_tag="ACHKV2_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6941969.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            2676..3152
                     /gene="yeeS"
                     /locus_tag="ACHKV2_004193"
     CDS             2676..3152
                     /gene="yeeS"
                     /locus_tag="ACHKV2_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6941970.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3215..3436
                     /gene="yeeT"
                     /locus_tag="ACHKV2_004194"
     CDS             3215..3436
                     /gene="yeeT"
                     /locus_tag="ACHKV2_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6941971.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3510..3878
                     /gene="cbeA"
                     /locus_tag="ACHKV2_004195"
     CDS             3510..3878
                     /gene="cbeA"
                     /locus_tag="ACHKV2_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO6941972.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3967..4340
                     /gene="cbtA"
                     /locus_tag="ACHKV2_004196"
                     /pseudo
     CDS             3967..4340
                     /gene="cbtA"
                     /locus_tag="ACHKV2_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4337..4531
                     /gene="yeeW"
                     /locus_tag="ACHKV2_004197"
     CDS             4337..4531
                     /gene="yeeW"
                     /locus_tag="ACHKV2_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6941973.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4577..4657
                     /locus_tag="ACHKV2_004198"
                     /pseudo
     CDS             4577..4657
                     /locus_tag="ACHKV2_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4946..5092)
                     /locus_tag="ACHKV2_004199"
                     /pseudo
     CDS             complement(4946..5092)
                     /locus_tag="ACHKV2_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5146..5328
                     /locus_tag="ACHKV2_004200"
     CDS             5146..5328
                     /locus_tag="ACHKV2_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO6941974.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5429..5758)
                     /gene="tmaR"
                     /locus_tag="ACHKV2_004201"
     CDS             complement(5429..5758)
                     /gene="tmaR"
                     /locus_tag="ACHKV2_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO6941975.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5930..6988)
                     /gene="yeeA"
                     /locus_tag="ACHKV2_004202"
     CDS             complement(5930..6988)
                     /gene="yeeA"
                     /locus_tag="ACHKV2_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6941976.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7187..7660)
                     /gene="sbmC"
                     /locus_tag="ACHKV2_004203"
     CDS             complement(7187..7660)
                     /gene="sbmC"
                     /locus_tag="ACHKV2_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO6941977.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7779..8945)
                     /gene="dacD"
                     /locus_tag="ACHKV2_004204"
     CDS             complement(7779..8945)
                     /gene="dacD"
                     /locus_tag="ACHKV2_004204"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO6941978.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            9154..10581
                     /gene="sbcB"
                     /locus_tag="ACHKV2_004205"
     CDS             9154..10581
                     /gene="sbcB"
                     /locus_tag="ACHKV2_004205"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO6941979.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(10624..10851)
                     /gene="tsuB"
                     /locus_tag="ACHKV2_004206"
     CDS             complement(10624..10851)
                     /gene="tsuB"
                     /locus_tag="ACHKV2_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO6941980.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10865..11914)
                     /gene="tsuA"
                     /locus_tag="ACHKV2_004207"
     CDS             complement(10865..11914)
                     /gene="tsuA"
                     /locus_tag="ACHKV2_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO6941981.1"
                     /translation="MILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLI
                     AISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEG
                     LIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVI
                     TLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT
                     GRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADA
                     QTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVY
                     VRPQRKARLATAAAN"
BASE COUNT         3119 a         3102 c         2844 g         2850 t
ORIGIN      
        1 ttgcacgtgc cggggaatat aaaaattaca gccgggatga aataaaatac tggcgatata
       61 catcatacaa gggggggaaa cttccggaag gtttcactga tgagaaattt tccagcgcca
      121 tttacaacgg aagaatattt acaatgaaac gtttacatac ccttatgtta tttctggcgg
      181 ttctgttcac gggctttaac tttaacgcag aagcggctac cgtaaaacaa gctcttagtt
      241 gtaacccgga agcctgggct gaacaacctg gagcgtgtcc atcaacgtac gagttgtacg
      301 aaggtgacgc tacctacaaa gccgctatcg acaaagcatt aaaaccggtc ggactgagcg
      361 gcatgttcgg taaaggcggc tatatggatg gccctggtgg aggtatcacg ccagtaaaca
      421 ttaacggtac agtctggttc cagggcgacg gttgcaaagc caatacctgc ggctgggact
      481 ttatcgtaac actctataac ccaaaaaccc atgaagttgt tggctaccgt tactttggtt
      541 tagatgaccc ggcctacctg gtttggttcg gtgaaattgg cgtgcatgaa ttcgcgtatc
      601 tggtgaaaaa ctacgtagct gcggttaact aatttaagga gtctatcatg aaaactttta
      661 tcaaaacttt actcgttgct gtaactattc tgttctctgt cttcgctact gcgaaacaag
      721 taaaactgcc aaataacatc aaatacgtta atactacaga agcgttttcc tgtaccgaaa
      781 ttgacggtat gaattgccag acaaagaatc agttcaacta taaagataac agctatgttt
      841 tcgtgcttga acgtggtggt gcctggtgct acgactacac tgtctcggta gttaacctga
      901 aaaccgggaa agcacaaatg attgaatacg gggacaatca actgtgctca ggtagcaaca
      961 aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg agtcatcgac acatccggaa
     1021 aacctgtcgg tgtagcccag gacaaactta aaatctaagg agtaaaccat gcgtttctct
     1081 gcacattatt aatatcaaag gtaaatacag ccccagccat tgttaccgga aagccgctta
     1141 atgcgtggct cgccagtgaa aggcctctca gacaataaac cttattcatt tcacccgtca
     1201 ggccgcgtct tctccgggag acgcggcttt tttcatttat tgcaccagta aatcttaacc
     1261 accgataagg agcaaagtat gcgattagcc agtcgttttg gttatgctgc aaaccagata
     1321 cgccgtgacc gtccgctgac acatgaagaa ctgatacgcc atgtacccag tatttttggg
     1381 gaagaccggc acacctcccg cagtgaacgg tatgcgtaca ttcccaccat taccgtcctg
     1441 gaaaatctgc agcgggaagg ctttcagccg ttcttcgcct gccagacccg tgtgcgcgac
     1501 ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg agagataaac
     1561 ggagaacatg tccctgaaat tattctgctc aactctcatg acggtacctc cagctaccag
     1621 atgctgccgg gttacttcag gttcgtctgc cagaacgggt gcgtctgtgg ccagtctctg
     1681 ggggaagtgc gtgttccaca ccggggaaat gtagtggaga aagttatcga aggggcttac
     1741 gaggtggtgg gcgtgtttga ccggatagag gagaagcgtg atgccatgca gtcgctggtc
     1801 ctgccgcaac cggcacgcca ggcgctggca caggcggcac tgacttaccg ttatggtgac
     1861 gaacatcagc ccgtcaccac cgccgacatt ctgacgccgc gacgccggga ggattacggt
     1921 aaggacctgt ggagcgcata tcagaccatc caggagaata tgctgaaagg cgggatttcc
     1981 ggtcgcagtg caaaaggaaa acgtatccat acccgggcca ttcacagcat tgataccgac
     2041 attaagctca atcgcgcatt gtgggtgatg gcagaaacga tgctggagag cctgcgctga
     2101 tgccgtttcc ctgagtgagg gaatcattca gctttcgttc ccgaaaaaca cacatttatt
     2161 tgttatttaa ggagttatct catgaaaacc gtttctcaga atacccccac aatttattca
     2221 gctacaacac cagagaataa tccgcctcag ttggttgcca gcctcgtccc tgatgaacag
     2281 cgcatcagct tctggccgca gcattttggc ctcattccac agtgggtcac cctggagccc
     2341 cgtgtcttcg gctggatgga ccgtctgtgc gaagactact gcggtggtat ctggaatctg
     2401 tacaccctga acaacggcgg ggcatttatg gcccccgaac cggatgatga tgatgacgaa
     2461 acatgggtac tgttcaatgc catgaacggt aaccgcgctg aaatgagccc ggaagccgcc
     2521 ggtattgccg cctgtctgat gacgtacagc catcatgcct gtcgtacgga gaattatgcc
     2581 atgacggtcc attattaccg gttgcgggat tacgccctgc agcatccgga aggcagcgcc
     2641 attatgcgca tcattgactg aaaggggccg gaataatgca acagatttcc tttctgcccg
     2701 gagaaatgac gcccggcgag cgcagcctca ttcaacgggc cctgaaaacc ctggaccgcc
     2761 atcttcatga acccggtgtg gccttcacct ccacccgtgc agcacgggaa tggctgattc
     2821 tgaacatggc gggactggag cgtgaagagt tccgggtgct gtatctgaac aaccagaatc
     2881 agctgattgc cggtgaaacc ctcttcaccg gcaccatcaa ccgcacggaa gtccatcccc
     2941 gggaagtgat taaacgcgcc ctgtaccaca atgccgctgc cgtggtactg gcacacaatc
     3001 acccgtccgg tgaagtcaca cccagtaagg cagaccggct tatcaccgaa cgtctggtac
     3061 aggcactggg cctggtggat atccgggtgc cggaccatct gatagtcggt ggcaaccagg
     3121 ttttctcctt tgccgaacat ggtctgcttt aacccgtcac aaccacatca cacctgtttt
     3181 cacttttatc ttctgtcttc agaggtatcc cattatgaaa attatcaccc gtggtgaagc
     3241 catgcgtatt caccaacaac atccgacatc ccgtcttttt ccgttctgta ccggtaaata
     3301 ccgctggcac ggcagtgctg aagcgtatac cggtcgtgaa gtgcaggata ttcccggtgt
     3361 gctggccgtg tttgctgaac gccgtaagga cagttttggt ccgtatgtcc ggctgatgag
     3421 cgtcaccctg aactgagtgg gaattctgat gagcagaatt atcgccacta ccggaccatt
     3481 cttagccgat tttctgtaag gattttatcg tgtcagacac actccccggg acaacacttc
     3541 ccgacgacaa tcacgaccgc ccctggtggg ggctgccctg caccgtgacg ccctgtttcg
     3601 gggcacgtct ggtgcaggag ggtaaccggt tgcattacct tgccgaccgc gccggtatca
     3661 gaggcctgtt cagcgatgca gatgcgtacc acccggacca ggcctttccg ctgctgatga
     3721 aacaactgga actcatgctc accagcggtg aactgaatcc ccgccatcag cataccgtca
     3781 cgctgtatgc aaaagggctg acctgcaaag ccgataccct cagcagttgt ggttacgttt
     3841 atctggctgt ttatccgacg cccgaaatga aaaattaact ctccagaata gccttctgct
     3901 acggcctggt gttttcacca cgccactttt ccatttttat atctgcatat caggaaaatc
     3961 ttcagtatga aaacattacc tgtattaccc gggcaggcgg ccagttctcg cccgtctcct
     4021 gttgaaatct ggcagatact gctgtcccga ctgctggacc agcactatgg cctcacactg
     4081 aatgacacac cttttgccga tgaacgtgtg attgagcagc atattgaggc aggcatttca
     4141 ctgtgtgatg cggtgaactt tctcgtggaa aaatacgcgc tggtgctacc gaccagccgg
     4201 gattcagcgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc cgggctcgca
     4261 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     4321 agtatccgga ggcgaaatga tgacactgga agccgacagc gttaacgtac aggcgctgga
     4381 tatggggcac attgtcgttg acattgatgg tgttaatatc actgaactga ttaataaggc
     4441 cgctgaaaac ggttattcac tccgcgtggt ggatgaccgt gactctaccg aaacaccggc
     4501 aacttatgcc agccctcacc agttgctgta agacaatgca gtgatgcagg gtatagtgcc
     4561 attaaccaca gaaacagcac tgttgtcctg tattaagcag gcaagagaag atgcccttac
     4621 cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca ggcttcattt
     4681 cccctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa ggaaaaccga
     4741 taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg ataaaccttc
     4801 cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa aaatgaattc
     4861 caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga ttaaagtaag
     4921 cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc catggcagga
     4981 gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg cgcagatacg
     5041 cttccggatc gataccgttc agacggcagg tgcctgcttt tccggcaaca agcaggaatc
     5101 tcagcgctga agcaggaatt agaggtaaaa acgccctacc gggccatgaa tcacccggtc
     5161 attggagtag taaccaaagc agatttagcc agcatggaac aaatatcttt ggtgaaatgt
     5221 tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcggttaa taacaatgga
     5281 gttacagaac tttttgcctt gctgcataca gaagaaggct gccgctaatt tcgataggtt
     5341 ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat gagggttgcc
     5401 gggcaaccct cattgaataa aacaggaatt acttcgcttc gccgtttttc atttcgccca
     5461 tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga
     5521 tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg
     5581 cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac aggacgcgct
     5641 tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac
     5701 gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt
     5761 ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca
     5821 acattactca tagctgccgc agatgacaat gcttttatcc cttatcacta tcataccctt
     5881 tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca
     5941 aggcccggca aatcagactc gatagcaact caagctgatg tgccgtttcc atgttcagcc
     6001 acacataacc atagattggt gtttccacaa ccttcaggtc atggtggtta ttcagcaact
     6061 gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat
     6121 taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat
     6181 cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtatgcat
     6241 tgatttgcaa ctccagcata caaaccagat tgcggttaat ggtctggata ccttcatata
     6301 tcgattttgg aatacgagtt tctttgctgg cgggcgcaat cagcccacgc attttcacgg
     6361 catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg
     6421 agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc
     6481 agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga
     6541 ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa
     6601 caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc agcgccagcc
     6661 aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac gaaattaact
     6721 ccagttgcag agcgataaga cctaaaatcg accccaacac cgtaccgcca atacgttcga
     6781 aggcacgagg gacaacgttc ccccagaacg agattggccc cataatcacc accatggtga
     6841 ccagcggcca ggtgccttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga
     6901 atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa
     6961 acgggcttaa tgacttattg gcacgcacgc agaaactccg ttaatgaaga ttaaaagcgc
     7021 tcataacaca tctgtcggtc agaaggcgcg cctctgaatt atccgacgga caaaccggga
     7081 acagtgtaat ttcccggcag gagcgaaaaa attgaaaggc gcatcttact cttttcgctt
     7141 tcaaaaaaag agtggtcttc gcgtcaacac accgccctga gatgaattag tgatgttttg
     7201 gctgcaccgc aacatacatt tcgatatccc agtacccatc ttccgcgcca ttgttcaagt
     7261 aaacctcgaa gcagggcttt ggtaacattt cataagcact gtcctgcaag aggctattaa
     7321 agaactgata ccagggttta gcaaaatcat caccgactac acgagccacc gccaccgcat
     7381 attgaccacc tgaaatttct gtcagaatga cgccctcact gttttcgggg agcgtaaagt
     7441 tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt tcatccggat
     7501 tgtcgtaata gacagcaacc cactccttcg gcacaatatt tttgctatct acccacatca
     7561 tcaactgctc aaaacctttc tttaccgtct gttcccacgg gccaacgaga tggaaacctg
     7621 caacggtacg tttatcttcc tgcttaatct cgtagttcat gacgcctcca ttattactgt
     7681 ttttatatac agtatagttg caatattaaa accacaagga atgagtgttg attatgcgag
     7741 cagactcgca ctcctgccag tctgctgcaa aagaaaggtc aggccttatg gtggaaataa
     7801 tcactcaggc gagaaaacat gctgccttcc ccgacagatt ccagggtaac cagcggccag
     7861 tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg atgggcgcta
     7921 attggcgcgg tgagctcttt accatcaagg gtatatttgg ctttgatatg tggaatttcg
     7981 gctttcggta gcaccatcca gaactcttgt tccgttccca gggcgatatt ttctttatcg
     8041 ccataccaga tgcgttccgt accgaccttt ttcccacggt gcaaaatttg caccgtagta
     8101 aagttttgtt gcccccaacg cagtaatttt cttgcctctt cctcacgacc ttttgcactg
     8161 tcagccccca ttaccactgc aatgagacga cgctgcccat ctacagccga agcaatgaga
     8221 ttaaacccgg caccagaagt atgacccgtt ttcaggccgt caacattcat ggttttatcc
     8281 cacaataacc cgttacggtt ttgctgggtg ataccgttcc aggtgagact tttctcactg
     8341 tacatatgat aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac agctaaatca
     8401 taagccgagc tatgctgacc tggtgcatcc agaccatgca ctgtttcaaa atgcgtatcc
     8461 ttgagatgca gcttctcggc atagttgttc atcatttcaa caaactgccg ttgcccaccg
     8521 gcaatatagt cagccagtgc aacacaagcg tcatttccgg aatccacaat taaaccacgg
     8581 cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa acatcagtga agaaccgaca
     8641 aacactggat tatctttcgc ccacgcatcg cgccccacgg tgacaatatc gtctggcgta
     8701 atgcgatgac tatcgatagc gcgatccacg acatagcccg tcatcagctt tgtcaggctg
     8761 gcgggattgc gctgttgatg ctcattaccc gcggtgagga tctgaccggt ggtgtaatcc
     8821 atcaataccc aggagccggc atgaatctct ggaggctgag gtgaaaaagg aatgttttcc
     8881 gccgcaaaac cagacgataa gttaaaaatg aacaaagaag cagcaataat aagacggcgt
     8941 ttcaacagca aaccctcagg agtttcaaat agctgttctt tttacggaaa tacttatgaa
     9001 ctggctggaa taaagtgcaa gaaaatgtga ctaccctctc atttttatct gacatgatct
     9061 gttgccactc gctgtcaaat tgtcgcgcta aagctgatta gcacggtgat atttgatact
     9121 ctggcagaca gcagaaacaa cggatttaac ctaatgatga atgacggtaa gcaacaatct
     9181 acctttttgt ttcacgatta cgaaaccttt ggcacgcacc ccgcgttaga tcgccctgca
     9241 cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca tcggcgaacc cgaagtcttt
     9301 tactgcaagc ccgcggatga ctatttaccc cagcctggag cagtattaat taccggtatt
     9361 accccgcagg aagctcgggc gaaaggagaa aacgaagccg catttgccgc ccgtattcac
     9421 tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg tttcgacgac
     9481 gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc cttacgcctg gagctggcag
     9541 catgataact cgcgctggga tttactggat gttatgcgtg cctgctatgc cctgcgcccg
     9601 gaaggaataa actggcctga aaatgatgac ggtctaccga gctttcgcct tgagcattta
     9661 accaaagcga atggtattga acatagcaac gcccacgatg cgatggctga tgtgtacgcc
     9721 actattgcga tggcgaaact ggtaaaaacg cgtcaaccac gcctgtttga ttatctcttt
     9781 acccatcgta ataaacacaa actgatggcg ttgattgatg ttccgcagat gaaacccctg
     9841 gttcacgttt ccggaatgtt tggggcatgg cgcggcaata ccagctgggt ggcaccgctg
     9901 gcgtggcatc ctgaaaatcg caatgccgta attatggtgg atttggcagg agatatttcg
     9961 ccattactgg aactggatag cgacacattg cgcgagcgtt tatataccgc aaaaaccgat
    10021 cttggcgata acgccgccgt tccggttaaa ctggtacata tcaataaatg tccggtgctg
    10081 gcccaggcga atacgctacg cccggaagat gccgaccgac tgggaattaa tcgtcagcat
    10141 tgcctcgata acctgaaaat tctgcgtgaa aatccgcaag tgcgtgaaaa agtggtggcg
    10201 atattcgcgg aagccgaacc gtttacgcct tcagataacg tggatgcaca gctttataat
    10261 ggctttttca gtgacgccga tcgtgcagca atgaaaattg tgcttgaaac cgagccgcgt
    10321 aatttaccgg cactggatat cacttttgtt gataaacgga ttgaaaagct gttgttcaat
    10381 tatcgggcac gcaacttccc ggggacgctg gattatgccg agcagcaacg ctggctggag
    10441 caccgccgcc aggtcttcac gccagagttt ttacaaggct atgcagaaga gattcagatg
    10501 ctggcgcagc agtatgccga cgataaagaa aaagtggcgc tgttaaaagc gctttggcag
    10561 tacgcggaag agattgtcta atgtgaagcc tccgccgctg gtacggcgga ggacaatgtt
    10621 gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg ccaatctgct gataatcggt
    10681 gatggcatgc ccttcttctg ccgcccactg tgggattgct tcagttgcct gggtgcaatc
    10741 aaactcaatt accaactcat cgccgctggc catttctgcc agtgctgctt ttgcttctat
    10801 gagtggaaac ggacagacct gcgtcaccac atccagcttt ttaatcgcca tctttacacc
    10861 ttacttaatt tgccgcagca gttgccagtc gcgccttacg ctgcggtcga acatacacaa
    10921 gccaggacgc agtccagact ccgagaatca taaatacaag gccaacccag ccctgccagg
    10981 tcatcattgc agtcataacc agtccattac cgatagagca accacctgca atactggcac
    11041 cgaagcccat cagtacacca cctagcccgc tacgtactgt tgtttgtgca tcagctgcgc
    11101 gaacgcggaa ctcacggctc gctttggctg caataaatgc ccccacgaag atccctaaca
    11161 ccaggaaaac gccccagtta atgtatttca cgtcacccgc gaccagaaat tgcagaatat
    11221 tggccgttgg ggaagtgatg cctaacccaa acatacgtcc ggttgcttca ctcaggggcc
    11281 aggctaaaag cgcaatcaaa ccgatgagta cagctgtgac aaagggatgc cagcgttttt
    11341 caaacagaat atgagcgatc ccggttcggc gcggcggtaa ggtcgcgact ttgagttttg
    11401 gcttcttcaa ctctttcatc accacccaga gcgttatcac cagcaaaacg gcaaccaacg
    11461 gccacacaga caaattaaaa gtctcagcaa tagagttatg ttcagtagtg tagtgctgca
    11521 aggtttgatt taaaccactg gcgtgtggag aacgcatcac cgcactcatc accatataag
    11581 tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc gcgataccag gtcccggtgg
    11641 cacatccacc cgccagaaca attcccagcc cgaagagata cccacctata acagtaccaa
    11701 gccacgggaa cgccccggct tcgtaagtca gtaggcccgc ctgaattaac gcaaaaaccc
    11761 ccacgctttg taccgaaatt gcaatcagca aggcgtaaaa catgcgatta tttttcgcga
    11821 tatacatatc gcgaaaacca ccagtcaggc aaaaacgccc acgctgcatg acaaatccca
    11881 gcagagcacc acaaattagc ccgcttaata tcatt
//
LOCUS       DAZFTN010000134        11856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFTN010000134 DAZFTN010000000
VERSION     DAZFTN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            188..481
                     /locus_tag="ACHKV2_004208"
                     /pseudo
     CDS             188..481
                     /locus_tag="ACHKV2_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(539..970)
                     /gene="tnpA"
                     /locus_tag="ACHKV2_004209"
     CDS             complement(539..970)
                     /gene="tnpA"
                     /locus_tag="ACHKV2_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFO6941982.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            978..2186
                     /locus_tag="ACHKV2_004210"
     CDS             978..2186
                     /locus_tag="ACHKV2_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6941983.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANICRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2230..2337
                     /locus_tag="ACHKV2_004211"
                     /pseudo
     CDS             2230..2337
                     /locus_tag="ACHKV2_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2321..2959)
                     /gene="ytfB"
                     /locus_tag="ACHKV2_004212"
     CDS             complement(2321..2959)
                     /gene="ytfB"
                     /locus_tag="ACHKV2_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO6941984.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3177..3797
                     /gene="fklB"
                     /locus_tag="ACHKV2_004213"
     CDS             3177..3797
                     /gene="fklB"
                     /locus_tag="ACHKV2_004213"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6941985.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4106..5518
                     /gene="cycA"
                     /locus_tag="ACHKV2_004214"
                     /gene_synonym="dagA"
     CDS             4106..5518
                     /gene="cycA"
                     /locus_tag="ACHKV2_004214"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO6941986.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5563..6225)
                     /gene="ytfE"
                     /locus_tag="ACHKV2_004215"
     CDS             complement(5563..6225)
                     /gene="ytfE"
                     /locus_tag="ACHKV2_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO6941987.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6333..7298)
                     /gene="ytfF"
                     /locus_tag="ACHKV2_004216"
     CDS             complement(6333..7298)
                     /gene="ytfF"
                     /locus_tag="ACHKV2_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6941988.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7406..8266)
                     /gene="qorB"
                     /locus_tag="ACHKV2_004217"
     CDS             complement(7406..8266)
                     /gene="qorB"
                     /locus_tag="ACHKV2_004217"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6941989.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8355..8735
                     /gene="ytfH"
                     /locus_tag="ACHKV2_004218"
     CDS             8355..8735
                     /gene="ytfH"
                     /locus_tag="ACHKV2_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO6941990.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8853..10796)
                     /gene="cpdB"
                     /locus_tag="ACHKV2_004219"
     CDS             complement(8853..10796)
                     /gene="cpdB"
                     /locus_tag="ACHKV2_004219"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO6941991.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10986..11726
                     /gene="cysQ"
                     /locus_tag="ACHKV2_004220"
     CDS             10986..11726
                     /gene="cysQ"
                     /locus_tag="ACHKV2_004220"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO6941992.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2816 a         2951 c         3081 g         3008 t
ORIGIN      
        1 ttttctgaag tggtgaaaat agcggatggt gaaagatatt aatatcaaaa atcgctcatt
       61 atggatataa cgatgcaccc ggggagaaaa tgacttccac gccttacacc gtacattctt
      121 gttgtgcggt atgtaattat tctacgctgt cggtatcggt gtattcgtta atcagcaggt
      181 taaccagcca caaaaagcca ttgagaaaat tattgatttt acatgggatt attatattgc
      241 taacccctgg tttttaaaaa ttgtgcatag tgaaaatcaa agtaaaggtg tgcattatgc
      301 aaaatcacag cgtctcttag aaatcaacca tgcgcacttg cagttaatgg aatccttatt
      361 ggatgaaggg aagaaataca atatttttaa gccagatatc gacccattgc aggtgaatat
      421 taatattgct gcgcttgggg gatattattt gatcaaccag catacgcttg gcctggttta
      481 tccggtgcgc cgtaaaaccc cgtccttcag ggcggggata tacggcgcgg ttttccacct
      541 aaccaggtgt ttgctgttgt tcaatgtatt gacggatggc ggatattggc gcacctccgc
      601 agctactggc aaaatagcca ggactccaca aaacgccttt gtagtaatac ctgactgcaa
      661 tatctggtcg atctcgtcgc agtaacctac ccgatacgcc tttgaggctg tttaccagac
      721 tggatatcgc cagtttggga ggatagttaa ttaacaaatg gacgtgatct ggttcgccat
      781 ccatttcaac cagttcagct tcaaaatcag cacatacctt tgaaaagtaa gtgcgtagtt
      841 tttctgtcgc gtcatggtca aaaatctggc gtcggtatct ggtgacaaag accaggtgaa
      901 catgcatcag gaaaacacaa tgccttccac gccggatatc ggtttctttt ttcacagacc
      961 aaagtatcat ttggcctgtg aaacgactac aggcatttaa attccagtta agacccggtg
     1021 gtcaacagga gcgtgaaatg aggcgcttcg caggcgcatg tcgtttcgtt ttcaatcgtg
     1081 ctctggcgct tcagaatgag aatcatgagg ccggtaataa atacatccct tacgggaaaa
     1141 tggcttcctg gctggttgag tggaaaaatg ccactgaaac gcaatggctt aaagatgccc
     1201 cgtcacagcc attgcaacag tcactgaaag accttgagcg ggcttacaaa aacttcttcc
     1261 ggaagcgggc tgcttttccc cgattcaaaa agcggggaca gaatgatgca ttccgctacc
     1321 cgcagggcgt taagctcgat caggaaaaca gccgtatttt tctgccgaaa cttggctgga
     1381 tgcgctaccg gaatagccgt caggtcacgg gtgttgtgaa aaatgtcact gccagccagt
     1441 cctgcggtaa gtggtacatc agtattcaga ctgaaaatga agtatcaact ccggttcacc
     1501 cttcagcatt aatggtcgga ctggatgctg gcgtggctaa actcgccacg ctgtcagatg
     1561 gcacagtctt tggacctgta aacagttttc agaaaaacca gaagacgctg gcgagacttc
     1621 agcgacagtt aagccgcaag gtcaaattca gcaacaactg gcagaagcag aaacgcaaaa
     1681 tacagcgact gcattcctgt atcgcaaata tctgcaggga ctaccttcac aaagtcacaa
     1741 cgaccgtcag caaaaaccac gcaatgatag tcattgagga tttgaaggtc agcaacatgt
     1801 caaagtcagc agcgggtacg gtcagccagc cggggcgcaa tgtccgggca aaatcaggtt
     1861 taaaccgttc gatactggat cagggctggt atgaaatgcg ccgccagctt gagtacaagc
     1921 agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtacacaagc cagcgttgcg
     1981 cgtgctgtgg tcatacagcg aaagaaaatc gcctgtcaca aagtaaattc agatgccagg
     2041 catgtggata tacagcgaac gctgatgtaa atggcgctcg taacatttta gcggcggggc
     2101 acgccgttct tgcctgtggg gagatggtgc agtcaggccg cccgttgaag caggaaccca
     2161 ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc cacgtccttc agggcttgga
     2221 ggatgtcaac acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc
     2281 aaagagacaa tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac
     2341 tgccatccgg ctggcgggtg aacaggacct gttgattatt ccccgtatca atggttaagc
     2401 ccgtcaccac gccgctggcg ttctggcgga ttttcaccat ctgcccgttt tgcaaattac
     2461 tcagcggctt acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc
     2521 cgtggtcgcg gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt
     2581 gattatcaat tccgctatct ggctggaatg gttgcgtttg ggtagttttc ggctgttctt
     2641 ctggctggcc ttcctgtata ggttctggag cgaccggagc cacctgatca ggataatttt
     2701 gtggggtgac taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa
     2761 tatctaactg cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca
     2821 gaaatccgac caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca
     2881 gcgggtccat gaagcgaaaa ttgtccggtg cgtgccagac tttctccagg gttggtttta
     2941 gttcaaagcg cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct
     3001 atgaataagc taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga
     3061 tattgctatt gtttcttttt ccctgggatt tgtcatcatt cccgcgacaa agttttaccc
     3121 aaagaagtgt ggctgatatg ctgcccgcta ctttataccc taagaaagga aatacgatga
     3181 ccaccccaac ttttgacacc atcgaagcgc aagcaagcta cggcattggt ttgcaggtag
     3241 ggcaacaact gagtgaatct ggcctggaag ggctgctgcc agaagcactg gttgcaggta
     3301 ttgccgatgc gctggaaggc aaacatccgg ctgttccggt tgatgtggtg catcgcgcgc
     3361 tgcgtgaaat ccacgagcgc gccgatgccg ttcgtcgtca gcgtttccag gcgatggctg
     3421 ctgaaggtgt gaaatacctg gaagaaaacg ccaaaaaaga aggtgtgaat agcaccgaat
     3481 ctggcctgca attccgcgtg atcaaccagg gtgaaggcgc aattccggca cgtaccgacc
     3541 gcgttcgtgt tcattacacc ggtaaactga tcgacggcac cgtgtttgac agctccgttg
     3601 ctcgtggtga acccgctgaa tttccggtta atggcgtgat ccctggctgg attgaagcac
     3661 tgactctgat gccggtaggt tctaaatggg aactgactat cccgcaggaa ctggcatatg
     3721 gcgagcgcgg cgcaggcgca tccatccctc cgttcagcac cctggtgttt gaagtcgaac
     3781 tgctggaaat cctctaagca gcgcattctg ttcccctcga accagagggg agcaggcatt
     3841 cagcaataaa cccttcagtt tgccaaacgg cgctattttg tgttgcaaag accccgtaag
     3901 cgtgtatttt tgtgagctgt ttcgcgttat caccgtgata tgacactcac tttaaacata
     3961 aaattaacat tagatctaaa tcttagtatt catcccgcgt attgttacct aatatcgatg
     4021 agtcccgata cagattcgtc gtatcataga ctgactaaag gccgtagagc ctgaacaaca
     4081 cagacaggta caggaagaaa aaaacatggt agatcaggta aaagtcgttg ccgatgatca
     4141 ggctccggct gaacagtcgc tacggcgcaa tctcacaaac cgacatattc agcttattgc
     4201 cattggtggt gccattggta cggggttgtt tatggggtct ggcaaaacga ttagccttgc
     4261 cgggccgtcg atcattttcg tttatatgat cattggtttt atgctctttt tcgtgatgcg
     4321 ggcaatgggg gaattgctgc tttcgaatct ggaatacaaa tcttttagtg acttcgcttc
     4381 cgatttactc gggccgtggg caggatattt caccggctgg acttactggt tctgctgggt
     4441 tgtaaccggt atggcagacg tggtggcgat cacggcttat gctcagttct ggttccccga
     4501 tctctccgac tgggtcgcct cgctggcggt gatagtgctg ctgctgacgc ttaacctcgc
     4561 cactgtgaaa atgttcggtg agatggagtt ctggtttgcg atgatcaaaa tcgtcgccat
     4621 tgtgtcgctg attgtcgtcg gcctggtcat ggtggcgatg cactttcagt caccgactgg
     4681 tgtggaagcg tcattcgcgc atttgtggaa tgacggcggc tggttcccga aaggtttaag
     4741 tggcttcttt gccggattcc agatagcggt tttcgctttc gtggggattg agctggtagg
     4801 tacaacagct gcggaaacca aagatccaga gaaatcactg ccacgcgcga ttaactccat
     4861 tccgatccgt atcattatgt tctacgtctt cgcgctgatt gttattatgt ccgtgacgcc
     4921 gtggagttcg gtagtcccgg agaaaagccc gtttgttgaa ctgtttgtgt tggtaggttt
     4981 gcctgcggct gccagcgtga tcaactttgt ggtgctgacc tctgcggcgt cttccgctaa
     5041 cagtggcgtc ttctctacca gccgtatgct gtttggtctg gcgcaggaag gtgtggcacc
     5101 gaaagcgttc gctaaacttt ctaagcgcgc agtacccgcg aaagggttga ctttctcgtg
     5161 tatctgtctg ctcggcggcg tggtgatgtt gtatgtgaat cctagcgtga ttggcgcgtt
     5221 cacgatgatt acaaccgttt ccgcgattct gtttatgttc gtctggacga ttatcctttg
     5281 ctcgtacctt gtgtatcgca aacagcgtcc tcatctacat gagaagtcga tctacaagat
     5341 gccgctcggc aagctgatgt gctgggtatg tatggcgttc tttgtgttcg tggtcgtgtt
     5401 gctgacactg gaagatgaca ctcgccaggc gctgctggtt accccgctgt ggtttatcgc
     5461 gctggggctg ggctggttgt ttattggtaa gaagcgggct gctgaactgc ggaaataacc
     5521 gcattatcat gctggatggc gcaatgccat ccagctttta gatcactcac ccgccagcgc
     5581 ccttgggaac agtacattgt tttccaggct gatgtgatcc atcaggtcat caatcagttc
     5641 attaatgccg ttatacatcg ctttccaggt ggtgcaggct tccggcggcg gtgtgacgtt
     5701 attggtattg tgtttgatca cttccagcag ttcacccgct tcatcgtgct cgctttccat
     5761 tacgctgatt ggccccattg cctggctgcc catgccttgt ttgatcatcg ggaagaggat
     5821 ctgctcttct ttcatcatgt ggctggaaag ctcttcatgc agcatggtca ggtattttgt
     5881 cagccctttt ggcacgctcg gtttgtcggc gtgaacgcgc tcgactttag tcgcttgcag
     5941 aatcagctcc ggcagttgct cgcggtgacg agcgtggtag cgcacgatga tatggtcgat
     6001 gatttctgcc agcggggcgc tacgccagtc tttctcaatc ggttgttcag caagctttgc
     6061 cagctcagct tcaatgacct caacatccag ttctttacgt gccgccgcac gcgccagcgt
     6121 ttgcttaccg ccgcagcagt aatccatatc atatttacga aacagagccg aagcgcgagg
     6181 aatagagagc gccagttcac ctaaaggttg gtcgcgataa gccatagctg atacctcatt
     6241 cttaataaga taagatgtat tttaaatgca tctttaaggc aaaagctata acccttacgt
     6301 agcagaggct tatttaactc actgccagcg tgtcaacttt ctgagacaga ttcagttaaa
     6361 ggtttttctg gttttgctct aaccgcgatg accacgccaa tcactaacag cgcgataccg
     6421 ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa aggtgtacag caaacctgcc
     6481 agcgtttcga aaacaatcag cggcccgaga atcactgtcg gtaatcgctg gctggcgacg
     6541 ttccagcaga gtgcgccaac ccatgagcaa agcacggcta tcgcaatcat cagactaata
     6601 aacaccagcg gacgggggcc aaagggtagg gagaagtccg gcgtttgcgt attcagccag
     6661 taacaagcga cgagatagcc gataagtgaa accggcagtg tgaccagcgc ctgcgctgtt
     6721 gcccacatca tcggatgttt gtcgggattt tcccgcagcc agcgggcgtt gcgcagggca
     6781 taccacgccc agcagaccac ggaaactaac gccagcacga tgccagaggt ataacgtgcc
     6841 cagtcaaaat tggggagtcc gtggtttaac tcagcaatat tcacacacgc caggccgatg
     6901 ccaatacaaa tcagtgccgg ggcgagtttt ccccacgcga gtttgccgtc gcgctggcta
     6961 taaagcagat tggcgaagac gggaatcacc accggcaggg tgccgataat catcgtggaa
     7021 acaggcgcac cagtacgttg aatggcactg gcaaggcaga agtaatagat gaggttgccc
     7081 atcatagtga gcatcaaggc ggtaagccag tcgcgacgcg ccaactgacg cagacgcacg
     7141 cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg ccagataacg ccccatcgac
     7201 tgcaacatcg ccgggtattc cggcacgatc aacggcccga caaaaataag cccccacatc
     7261 aaccctgcta acagggcgta cagcacgccg ctaatcatta ctggcatcca ttgatctgtc
     7321 agaagaaagt tcaggaagca gacatgattg ctcccggcat cccgtcatta tcagagagga
     7381 tgcgggagga tgccacttta attaactagt tattaacatt aaaaagatgg cttacgcttt
     7441 cggctaacgt tgtcgttggg tggccaatca atttgctaag tgttttgctg tcatcaaaca
     7501 gaccgccttt cgatgcgcca acgtcagaat ccgccagcat atccgccagt ccgtcgggca
     7561 gtccgacgct tttcagtgcc gcggcgaaat cggcttcgct cagattttga taggtaacct
     7621 gtttgccgct ctgtttggtc agctctgccg ctaactgtgt caacgtccag gcactatcgc
     7681 ccgccagttc gtaaaccttg ccttcgtgac cggcttcgct aatcacgcgt gccgcagctg
     7741 ccgcataatc tgcccgcgtt gctgaggcga ttttgccatc gcccgccgca ccgataaata
     7801 cgccgtgttc cagtgctgcc ggggcgctgg cgaggtagtt ttcggtgtac cagccgttgc
     7861 gcagtagggt gtaaacgatg ccagaatcag ccagcatttt ctccgtctcg atgtgctcat
     7921 cggcgaggcc gagcggagat ttatccgcgt gaagcaggct ggtataagcg ataaatttca
     7981 cgccagccgt ctttgcggca ttaataacat tacgatgctg cggggcacgt tgacccactt
     8041 cgctggaaga gatcagcagt agtttttcca ctccctgaag tgcagatgtc agtgcggctt
     8101 catcgccgta gtcagcctga cgcacggtaa tgccttgtgc tgtcagtgcc tgggcttttg
     8161 ccggattacg aacgatagcc actatttggc tggcaggaac cgttttcatc aaggattcaa
     8221 taacatactg accaagttgg ccagtggcac cagtaatagc gatcatggga agtctccatc
     8281 gttttgtctt gtgtttaaat acgctaacac ctaaacttac ttttagtaag tacgcacaaa
     8341 aaggtaagta tgaaatgagt caggttagcc tgtcgcaaca actgaaagag ggtaatctct
     8401 ttgcggaaca gtgcccgtcg cgcgaggtgt tgaaacacgt caccagccgt tggggggtgt
     8461 tgattctggt ggcgctacgc gaaggtactc atcgctttag cgacctgcgg cgcaaaatcg
     8521 gcggggtgag cgaaaagatg cttgcgcagt cgttacaggc gttagaacag gatggttttc
     8581 ttaaccgtat cgcgtatccg gtggtgccgc cgcatgttga atacagcctc acgccgctgg
     8641 gcgagcaggt gagcgaaaag gttgctgcac tggcggactg gattgagttg aatttgcccg
     8701 aggtgttggc ggtgcgggat gaacgtgcgg cataactgcc ggatgcgcgg cctacggtgt
     8761 atgacttgta gggcggataa gatgcgccag catcgcatcc gccaataaat gccggatgcg
     8821 attgatgatt tataggccga aaaagaagtg ttttacttac tcaaatccac ctgataaatc
     8881 gcaaacccga tatcatcggt cgcgacttta ttcatcggat actgcccttt ctctttaata
     8941 aacgctgcgg ctttatctga cggggaagtt tcgaaacgga tatccagttt cttatcggca
     9001 gctatcggtg ctaaacgcca gttgttatct gccgccgggt gaatttcccc cgcacgtttc
     9061 gactcatcag caatccacgc tgccagcacc gagcggttct catccggtga agcaaaagcg
     9121 atatggctgt cgcccgtccc ggcaaatttg ccgccgtaag cgcgatagtt attggtggca
     9181 acgaggaaca tagcgttcgg atcaatcggc ttgccattaa aggtcaggtt cttaatcctt
     9241 tccgcattgg cattaatcat ctggcactcg ccgtcataac gggcgggctg ggtaacatca
     9301 atctgataat tcacaccatc aataacatca aagttataag tgcggaaacc atcccagttg
     9361 atgagtgact gtggtttcgt gctgttggga tcaatctggt taaactgtcc ggcggagcac
     9421 tccagccact ctttcacctc tttaccgctg gctttcacca caatcagcgt attgggatag
     9481 agataaagat cggcggcatt acggaaggtc aactggcctt tttccacctc cacatagctt
     9541 gccgggtcat ttttgcgacc accgacttta aacggtgcgg cagctgaaag caccggcagt
     9601 tttgccagat ccggatcgcc ctgaatgtaa tgctcgacat acgctttttg cgcgttgttc
     9661 actacttgca cggtcggatc gtcctgcacc agcgccagat agctatacat attgtcggca
     9721 gatttaccga ttggcttgct gacgaactgg cgtgtggcat cgtgatcggc tttgagtgtt
     9781 tctaccagct tgctgtcttc cgccgcgagg gattttttat tggcgatgtc gtaaatcggc
     9841 cgtgcttccg ctttcgcctg cgtcacctgc catttaccgc tgtcattact gagttgtaag
     9901 tcgaccaccc caagatgatc gccccacatg cccggcatta ccgccggaac accattcagc
     9961 gtgcctttgg tgatatcagc cccttcgata tcagcaaaat ctttacccgg gaaaacggcg
    10021 tgagcatggc caaacataat ggcgttaacg cccggaattt cactgaggta ataaactgag
    10081 ttttccgcca ttactttata cggatcggca gacagcccgg aatgcgccag aacgacaacg
    10141 acatcggcac ctttctcgcg catttcaggc tcgtatttgc gcacggtttc ggtaatatca
    10201 ttcaccgtca cttttccgga taaattagct ttatcccagc ccatgatttg cggcggcacg
    10261 acgccaatat agccaatctt cagcgtctgt ttttttccgt ctttatcgac cacttcggtg
    10321 tctttaatta aatacggtgt aaacattggc tgtttggttc tggcgtcaat gacgttggca
    10381 tttacataag ggaatttcgc tcctgccagc gcatttttca ggtaatccag accgtagtta
    10441 aactcgtggt tgccaagcgt tccgacggta tagtccagcg tatttaatgc cttatagacc
    10501 gggtgaatat cacctgcttt taatcctttc gccgacatgt aatcggccag cggactcccc
    10561 tgaatcaaat cgccgttatc gaccagtacg ctgtttttca cttcattgcg ggcattgtta
    10621 atcaggcttg ccgtacgtac cagtccgaat ttttccgtgg cggtgtcttt gtaataatcg
    10681 aaatccatca tgttgctatg cagatcagtg gtttccatga tacgcagatc gaccgtcgct
    10741 gcattcacac tggcggcaat cagcgtggcc aggagcgttg cgctaaactt aatcatcagg
    10801 gacatccttt tatcatcggg aatacgaaag aaaagggaga ataaacgtct tacttataga
    10861 acagtgaaga atgccacaat tttacgcttt gaaaatgatg acactatcac agttggcgca
    10921 ttcattaatg atagggtata agtaaaacaa taagttaaca ccgctcacag agacgaggtg
    10981 gagaaatgtt agatcaagta tgccagcttg cacggaatgc aggcgatgcc attatgcagg
    11041 tctacgacgg ggcgaaaccg atggacgtcg tcagcaaagc ggacaattct ccggtaacgg
    11101 cagcggatat tgccgctcac accgttatca tggacggttt acgtacgctg acaccggata
    11161 ttccggtcct ttctgaagaa gatcctcccg gttgggaagt ccgtcagcac tggcagcgtt
    11221 actggctggt agacccgctg gatggtacga aagagtttat taaacgtaat ggcgaattca
    11281 ccgttaacat tgcgctcatt gaccatggca aaccgatttt aggtgtggtg tatgcgccgg
    11341 taatgaacgt aatgtacagc gcggcagaag gcaaggcctg gaaagaagag tgcggcgtgc
    11401 gcaagcagat tcaggtccgc gatgcgcgcc cgccgctggt ggtgatcagc cgctctcatg
    11461 cggatgcgga gctgaaagag tatctgcaac agcttggcga acatcagacc acgtccatcg
    11521 gctcttcgct gaagttctgc ctggtggcgg aaggacaggc gcagctgtac ccgcgtttcg
    11581 gaccaacgaa tatttgggac accgccgctg gacatgctgt ggctgcagct gccggagcgc
    11641 acgttcacga ctggcagggt aaaccgctgg attacactcc gcgcgagtcg ttcctgaatc
    11701 cggggttcag agtgtctatt tactaaattc agatggcaga aacagtgtat ttcctgattc
    11761 tgccatcctg atttctccca acctaaaaag ttataaataa aaagagattg tatttaaagt
    11821 gcaaaaattc aattgctaat aagttacatt ttaata
//
LOCUS       DAZFTN010000135        11745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFTN010000135 DAZFTN010000000
VERSION     DAZFTN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(87..410)
                     /locus_tag="ACHKV2_004221"
     CDS             complement(87..410)
                     /locus_tag="ACHKV2_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO6941993.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(418..603)
                     /gene="ynbE"
                     /locus_tag="ACHKV2_004222"
     CDS             complement(418..603)
                     /gene="ynbE"
                     /locus_tag="ACHKV2_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO6941994.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(600..3239)
                     /gene="ydbH"
                     /locus_tag="ACHKV2_004223"
     CDS             complement(600..3239)
                     /gene="ydbH"
                     /locus_tag="ACHKV2_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO6941995.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3447..4436
                     /gene="ldhA"
                     /locus_tag="ACHKV2_004224"
     CDS             3447..4436
                     /gene="ldhA"
                     /locus_tag="ACHKV2_004224"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6941996.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4547..4969
                     /gene="hslJ"
                     /locus_tag="ACHKV2_004225"
     CDS             4547..4969
                     /gene="hslJ"
                     /locus_tag="ACHKV2_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO6941997.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4966..5232)
                     /gene="ydbJ"
                     /locus_tag="ACHKV2_004226"
     CDS             complement(4966..5232)
                     /gene="ydbJ"
                     /locus_tag="ACHKV2_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO6941998.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5506..9030
                     /gene="nifJ"
                     /locus_tag="ACHKV2_004227"
     CDS             5506..9030
                     /gene="nifJ"
                     /locus_tag="ACHKV2_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO6941999.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9396..10509
                     /gene="ompN"
                     /locus_tag="ACHKV2_004228"
                     /pseudo
     CDS             9396..10509
                     /gene="ompN"
                     /locus_tag="ACHKV2_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            10650..11084
                     /gene="uspF"
                     /locus_tag="ACHKV2_004229"
     CDS             10650..11084
                     /gene="uspF"
                     /locus_tag="ACHKV2_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO6942000.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(11665..>11745)
                     /locus_tag="ACHKV2_004230"
     CDS             complement(11665..>11745)
                     /locus_tag="ACHKV2_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO6942001.1"
                     /translation="SIAVSMIVGRDNCAFDPDRGNFVLKN"
BASE COUNT         3009 a         2990 c         2789 g         2957 t
ORIGIN      
        1 cccacggtaa ttaagcatgc taacgtcagt ttaaatactg taaaagatca ttacattaat
       61 agatacattt tgtagccaac tataatttat ttgcgcaacc atttaccatt aactccctga
      121 acatattgcc ccgacttcgc gcgagcaaca agcttttgtc cagccagttt ggctatctct
      181 ttagtggata cattattttg ctttgccagt tgttggtaac tatggttacg agcttcatta
      241 atatctttta ctaatgcctg tgtttcggca tccgtttgta gcgccaccag gtaaccgtta
      301 agcgtttcac ctacccgccc ctgacttctg gcttcgttca gggttaaagc cattgcactg
      361 tcgctcatca ggccaatggc ccatacagct atgagtaatg ttcttttcat catcacctca
      421 aaatagatcg ctacgggttt tgagcaggtc ttcaacgtcc ttatccgctt taatgatgat
      481 ttcatgctca attttaacgt tcatattaat ggtgattggc tctttcggcg cagccacttc
      541 aatgcgaggc gtgcaaccaa ccaacataaa agaggacgca aacgcggcta tcactatttt
      601 cattgttttt cctcacactc ttttcctgat gggcagccag tttccggtag ctgggcattc
      661 tgctccagcc atacctggag attatcgcca aagcgtaaac tgcgccataa cgtaaaaata
      721 ttctcttcat ggtgataatt aaggtttacc gtaccgcttt taccatcgac gcgactggtg
      781 cccgaaatgt tggcctgcat ggttaacaca ccgagattat ctaaattaat ccttgttgat
      841 gagcgactga tttccatata acgcaaccag ttgatggcag ttccagcggt catattgtct
      901 ttggccaccg catctgcagt gtctttatcc agacgcagcg tcatcggccc cgcgtttgcc
      961 aaccagccat ctttgacaat ccatttttca ttgttcagcc atagtggcaa cgccccacta
     1021 aatgccccag acatagcaaa ctgtttcgga ttaaccgcgc taaccagttc gctggatgag
     1081 agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc
     1141 acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaatggttc ttcttcagtc
     1201 cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact
     1261 tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa
     1321 cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg
     1381 cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca
     1441 acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc
     1501 tggaatacag tcagtgattg tttaggccac caggcgttac cgcgcagacg aataccgtcc
     1561 cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg
     1621 aaatacgtcg gatcgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg
     1681 ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc
     1741 tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc
     1801 atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg
     1861 tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct
     1921 tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca
     1981 tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa
     2041 cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg
     2101 atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc
     2161 agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg
     2221 tccgcctgtt tcgcttcacc ggtcagctgc agaggcagct ggctgttatc catgcttaat
     2281 tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg ggtcagcaca
     2341 ctcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc gactttgaca
     2401 cccagtcggc cactcaaagg aaaacctgca tacggccagc tccagcgacc atcgcttacg
     2461 gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt
     2521 gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgac
     2581 ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc
     2641 atgagcggca tagtaaattc ccccaccagt ttaaccgacg gctgattttc aaacgcgacg
     2701 acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt
     2761 tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc
     2821 cattcctgcc agggagaaaa aatcagttta tcaatattga tccaggtgtt aggcagcatg
     2881 gactgccatt gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc
     2941 gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt
     3001 gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg
     3061 ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg
     3121 agcggtagcc agatgcccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc
     3181 aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg
     3241 ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat tgtggcatgt
     3301 ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat
     3361 atcgccatag ctttcaatta aatttgaaat tttgtaaaat atttttagta acttaaatgt
     3421 gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg
     3481 acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact
     3541 ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg
     3601 taaacgatga cggcagccgc ccggtgctgg aagagctgaa aaagcacggc gttaaatata
     3661 tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga cgcggcaaaa gaactggggc
     3721 tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta
     3781 tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt gacgctaact
     3841 tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc gttatcggta
     3901 ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg
     3961 cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc
     4021 caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca ccggaaaact
     4081 accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata
     4141 ccagtcgcgg tgcattgatt gattctcagg cagcaattga agcgctgaaa aatcagaaaa
     4201 ttggttcgtt gggtatggac gtgtatgaga acgaacgcga tctgttcttt gaagataaat
     4261 ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt
     4321 ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct cagactacgc
     4381 tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt
     4441 gccgctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc tttcattctc
     4501 tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga agaaagtagc
     4561 cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca aaattgctgt
     4621 tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg gtaagcccgt
     4681 gacctgcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga tttccggcag
     4741 catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga cagccaaagg
     4801 gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg ataacaccat
     4861 tagcgaaatg ctgaaagaag gtgcacaagt ggatctgacc gcgaaccagt taacgctggc
     4921 gaccgcgaaa cagacattaa cttataagct ggcggattta atgaattaat agctgccaca
     4981 gctcccggcg gcaagtgact gttcgctaca gcgtttgccg ttgggtaatg cacacatccc
     5041 aatcgccgta ccatccagtt gacgggcaac agaaagcgaa ccgccgatca ttgcacaatt
     5101 tgcttctcca ctactggaca tcgacgcttt taaacctggc gctacgtgcg ccgcagtcgc
     5161 ctgctgaaca ggctcactac tacacgccga caacaataaa gcggcacacc ctacccaaaa
     5221 cgctgctcgc atctcttctt cctctgatct tcaagccaaa cgacaccgcc ataaataata
     5281 ggcagcacag agggcgacgt cgagagctgt cctgcgcgtt gccccgcatt tttacttttt
     5341 tatggctatt tttttgccct ctgtttgatc aaaacattca ttacgctgat gtgggggaca
     5401 caaaagcgaa aatgcagaag aaagccattt gctaaaattg aaagattact actgggcgcg
     5461 cagcaatttc gtgcgcccct catttgcgca atgtaagggt gtcatatgat tactattgac
     5521 ggtaatggcg cggttgcttc ggtggcgttt cgcaccagtg aagttatcgc catctaccct
     5581 attaccccca gttccacgat ggcagaacag gctgatgcct gggccggaaa cggcttgaag
     5641 aacgtttggg gagacacacc acgcgtggtt gaaatgcagt cggaagcggg tgctatcgct
     5701 accgtgcatg gcgctttgca gacgggtgcc ctttcaacat cgtttacgtc atcgcagggt
     5761 ttgctgctga tgatcccgac gctgtacaaa ctggcaggcg aactcacacc gtttgtcctg
     5821 catgtagcgg cacgtaccgt tgccacacat gcactctcta tttttggcga tcattccgac
     5881 gtcatggcgg tgcgccagac gggttgcgcg atgttgtgtg cggcaaacgt ccaggaagcg
     5941 caagactttg ctctcatttc gcacatcgcg acgctgaaaa gccgcgtgcc atttattcat
     6001 ttctttgatg gtttccgtac gtcccacgaa atcaataaaa ttgtcccgct ggccgatgac
     6061 actattcttg atctcatgcc gcaggccgaa attgatgctc atcgcgcccg ggcgctcaac
     6121 ccggaacatc ccgtgatccg cggtacgtca gccaatcctg acacttattt ccagtctcgc
     6181 gaagccacca acccatggta caacgcggtc tatgaccatg ttgaacaggc gatgaatgat
     6241 ttctctgccg cgacaggtcg tcagtatcag ccgtttgaat attacgggca tccgcaagcg
     6301 gaacgggtga ttatcctgat gggctctgcc attggcacct gtgaagaagt ggttgatgaa
     6361 ttgctaaccc gtggcgaaaa agttggcgtg ctgaaagttc gcctgtaccg tcccttctcc
     6421 gccaaacact tgctgcaagc tctgccggga tccgtacgca gcgtggcggt actggacaga
     6481 accaaagaac ccggtgccca ggcagaaccg ctctatctgg atgtaatgac cgcactggca
     6541 gaagccttta ataatggcga gcgcgaaact ctgccccgtg tcattggtgg gcgctatggt
     6601 ctttcatcca aagaatttgg cccggactgt gtactggcgg tatttgccga gctcaacgcg
     6661 gctaaaccga aagcgcgctt tacggttggt atttacgatg atgtgaccaa tctgtcactg
     6721 ccgttgccgg aaaacaccct gccaaactcg gcgaaactgg aagccttgtt ttatggcctt
     6781 ggtagtgatg gcagcgtttc cgcgaccaaa aacaatatca agattatcgg taattccacg
     6841 ccgtggtacg cacagggcta ttttgtttac gactccaaaa aggcgggcgg cctgacggtt
     6901 tctcaccttc gcgtgagcga acagccgatt cgttccgctt atctcatttc ccaggctgat
     6961 tttgttggct gccaccagtt gcagtttatc gataaatatc agatggctga gcgtttaaaa
     7021 cctggcggca ttttcctgct caacacgccg tacagcgcag atgaagtgtg gtcgcgcttg
     7081 ccgcaggaag ttcaggccgt gctaaatcag aaaaaagcgc gcttctatgt gattaacgcg
     7141 gcgaaaatcg cccgcgaatg tggcctggcg gcccgtatta ataccgtcat gcagatggct
     7201 tttttccatc tgacacaaat tctgcctggc gatagcgccc tcgcagaatt gcagggtgcg
     7261 attgccaaaa gttacagtag caaaggtcag gatctggtgg aacgcaactg gcaggctctg
     7321 gcgctggcgc gtgaatccgt agaagaagtt ccgttgcaac cggtaaatcc gcacagcgcc
     7381 aatcgaccgc cagtggtttc cgatgccgcc cctgctttcg tgaaaaccgt aaccgctgcg
     7441 atgctcgccg ggcttggtga tgccctcccc gtttcggcgc tgccgccaga cggcacctgg
     7501 ccgatgggca ccactcgctg ggaaaaacgc aatatcgccg aagagatccc catctggaaa
     7561 gaggaactct gtacccaatg taaccactgc gtcgccgctt gcccacactc agctattcgc
     7621 gcaaaagtgg taccgcctga agcgatggaa aacgcccctg ccagcctgca ttcgctggat
     7681 gtgaaatcgc gtgatatgcg cgggcagaaa tatgtcttgc aggtggctcc ggaagattgc
     7741 accggttgta acctgtgcgt cgaagtttgc ccggcgaaag accgtcagaa tccagagatt
     7801 aaagccatca atatgatgtc gcgcctggaa catgtcgaag aagagaaaat caattacgat
     7861 ttcttcctca acctgccaga aatcgaccgt agcaaactgg aacgtattga tattcgcaca
     7921 tcgcagctga ttacaccgct gtttgaatat tccggtgctt gctccggttg tggcgagacg
     7981 ccgtatatta aattgctgac tcagctctat ggcgaccgga tgttgatcgc taacgccacc
     8041 ggctgttctt caatttatgg cggtaacctg ccctctacac cgtataccac cgatgccaac
     8101 ggtcgtgggc cggcatgggc gaactctcta tttgaagata atgccgaatt tggccttggt
     8161 ttccgcctga cggtcgatca acaccgtgtc cgcgtgctgc gtctgctgga tcaatttgcc
     8221 gataaaatcc cggcggaatt actgacggcg ttgaaatcag acgccacgcc agaggttcgt
     8281 cgtgaacagg ttgcagcttt acgccagcaa ctcaacgatg ttgccgaagc acatgaactg
     8341 ctgcgtgacg cagatgcact ggtggaaaaa tcaatctggc tgattggtgg cgacggctgg
     8401 gcttacgata tcggctttgg cggtctggat catgtattga gtttgacgga aaacgtcaac
     8461 attctggtgc tggatacgca atgctattcc aacaccggtg gtcaggcgtc gaaagcgaca
     8521 ccgctgggtg cagtaactaa atttggcgag cacggcaaac gtaaagcgcg taaagatctt
     8581 ggcgtcagta tgatgatgta cggtcatgtt tatgtggctc agatttctct cggcgcgcag
     8641 ctgaaccaga cggtgaaagc gattcaggaa gcggaagcgt atccgggacc atcgctgatc
     8701 attgcttata gcccgtgtga agagcatggt tacgatctgg cactcagcca tgaccagatg
     8761 cgccaactca cagctaccgg cttctggccg ctatatcgct tcgatccgcg tcgtgccgat
     8821 gaaggcaaac tgccgctggc gctggattca cgcccgccgt cagaagcact ggaagaaacg
     8881 ttacttcacg agcaacgttt ccgtcgtctg aattcgcagc agccagaagt ggcagaacag
     8941 ttatggaaag atgctgcagc tgatttgcaa aaacgctatg acttcctggc acaaatggcc
     9001 ggaaaagcgg aaaaaagcaa caccgattaa atgctctgga taaggattat ccaattctaa
     9061 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
     9121 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc gcgcagaata
     9181 acgtatagaa aattaaaagt ataattttta ttttgtatgt attatcttcg atacattcac
     9241 cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag gcgtaacaaa
     9301 tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc gaaatgggga
     9361 taaaaggcaa ataaaataac taaaggattt attcaatgaa aagcaaagta ctggcacttt
     9421 taattcctgc cctgctcggc gcaggtgctg cacatgcagc cgaagtttat aataaagacg
     9481 gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct gataattcag
     9541 cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc caaattaacg
     9601 atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac actgaatctt
     9661 caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaattttca gattacggtt
     9721 ctttcgatta cggacgtaat tatgggctgg accgatatgc tgcctgaatt tggcggtgac
     9781 tcttatacca atgcagacaa ctttatgact ggtcgtgcca atggcgtcgc gacttatcgt
     9841 aatactgatt tcttcggtct ggtaaatggt ctgaacttcg cggtgcagta tcaaggtaac
     9901 aacgaaggtg ccagtaatgg tcaggaaggc accaacaacg gacgtgatgt tcgccatgaa
     9961 aacggtgacg gctggggtct ttccacaaca tatgatttag gcatgggctt tagcgctggt
    10021 gcggcataca cctcttctga ccgcaccaat gaccaggtta accatactgc ggcgggtggt
    10081 gataaagcag acgcgtggac tgctgggcta aaatacgatg ctaacaatat ttacctggca
    10141 accatgtatt cagaaacgcg taatatgacc ccgtttggcg acagcgatta tgctgttgca
    10201 aacaaaaccc agaactttga agtcactgca cagtaccagt ttgattttgg tctgcgtccg
    10261 gcagtctctt tcctgatgtc taaaggccgt gacctgcacg ctgccggtgg tgcagacaac
    10321 ccggcaggtg ttgatgataa agatctggtt aaatacgccg atgttggcgc gacttactat
    10381 ttcaataaaa acatgtccac ttatgttgac tataaaatca acctgttgga tgaagatgac
    10441 agtttctacg ctgccaacgg catctctacc gatgatattg tcgctttagg tctggtttat
    10501 cagttctaaa tcctcctgcc cgctgttatg gcgggctttt tctgcttatt cttcctcctt
    10561 tgatctaaat taaaaatgtg aactccgtca ttacacaaaa agtgtcatct ggcgttacac
    10621 tttatgcgga tactaaaaca ggaggtttta tgaacagaac gattcttgtc cctatcgata
    10681 tttccgattc agaattaact caacgcgtga ttagccacgt tgaggcagag gcaaagattg
    10741 atgatgcaga ggttcatttc ctgacggtaa taccttcact gccctactat gcctctctgg
    10801 gtttagcgta ttccgcagaa ttaccggcaa tggatgacct gaaagcggaa gccaaatcgc
    10861 aactggaaga gatcattaaa aaatttaaac tgccaaccga cagagtgcat gtccatgttg
    10921 aggaaggctc gcccaaagac cgcattctgg aattggcgaa gaagatcccc gctcatatga
    10981 tcatcattgc ttcccatcga ccggatatca ccacttatct gctcggttcc aacgccgcag
    11041 ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg ctgacactaa cgcccgcaca
    11101 ttgctgcggg ctttttgatt catttcgcaa atgtgctgac attttcccct ctaatccgta
    11161 ccatacacgc cacagttttt atatcagatt tcttatgctg ggcgttccgg catgacgcat
    11221 actcttctga tgccatataa cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt
    11281 agcaagaaag aacaatacaa cctgaacaag tcagagtaaa aaccgcaact atctgacgaa
    11341 caataacttt tatgtttttc aatatgttag gcaggattaa caactacaat aaagcacaat
    11401 aatctacact ttttttgccc catttttgcc ccttttgcgg ccttttgtcc catttttgcc
    11461 acagtaaaaa tctataacta atcaatctgg cgcccatgat ttctttccaa tagaatcagt
    11521 tccggctgga aatcattaga tactcgcact atcgaaaagc ttaccagcca accgcggcac
    11581 gttcttgcat acgaggtgcc gcgttttaca tgccgtaccg agaattaatt tcattttacg
    11641 aaaagttaac ccgccattgc taccttaatt ttttaaaacg aagttacctc tgtcagggtc
    11701 aaaagcacaa ttatctcttc ctacaatcat tgaaactgcg attga
//
LOCUS       DAZFTN010000136        11731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFTN010000136 DAZFTN010000000
VERSION     DAZFTN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            212..4195
                     /gene="ehaA"
                     /locus_tag="ACHKV2_004231"
     CDS             212..4195
                     /gene="ehaA"
                     /locus_tag="ACHKV2_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO6942002.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4337..5425
                     /locus_tag="ACHKV2_004232"
     CDS             4337..5425
                     /locus_tag="ACHKV2_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6942003.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5467..6398)
                     /locus_tag="ACHKV2_004233"
                     /pseudo
     CDS             complement(5467..6398)
                     /locus_tag="ACHKV2_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6490..6997)
                     /locus_tag="ACHKV2_004234"
                     /pseudo
     CDS             complement(6490..6997)
                     /locus_tag="ACHKV2_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7255..7860
                     /locus_tag="ACHKV2_004235"
     CDS             7255..7860
                     /locus_tag="ACHKV2_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6942004.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7900..8763
                     /locus_tag="ACHKV2_004236"
     CDS             7900..8763
                     /locus_tag="ACHKV2_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6942005.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8753..10300
                     /gene="fdrA"
                     /locus_tag="ACHKV2_004237"
     CDS             8753..10300
                     /gene="fdrA"
                     /locus_tag="ACHKV2_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6942006.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10300..11718
                     /locus_tag="ACHKV2_004238"
     CDS             10300..11718
                     /locus_tag="ACHKV2_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6942007.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3101 a         2726 c         3169 g         2735 t
ORIGIN      
        1 atgataaagt taagagtgtt aacttaaaat aagtttttag tagtaacttg ctattttatt
       61 accctctgaa ctacatactc agatatgtgg ttgccaatgc tatgcaaaat ttttgaattt
      121 ttatatgcga tgaacagtaa ccggaatggc atttaatgcg ttgctgttta tgcaatcttg
      181 gttttatttg gatgtattgc tggagattgt gatgaataaa atatatcggc taaagtggaa
      241 caggtcccgt aactgttgga gcgtctgctc ggagctgggg agcagagtaa aaggaaaaaa
      301 gtcccgggct gttttaatta gcgcgataag tttatattca tctctggtat tcgccgatga
      361 tgtcatcgta aaccaggata aaactattga ttttggcaaa gagaaccaga gcatcgatta
      421 ccgtattacg gtgacagaca atgccaatct ggtaatcaat gcgacagata cttcccgtcc
      481 gcgtctgact ctcgcttctg gtggtgggtt ggatattacc ggaggaaagg taacaatcaa
      541 tggcccactt aactttttgc tgaaaggtac ggggttcctg aatgtctcca atgctggcag
      601 cgagttatat gctgatgatt tgtatgaatc aaactcaggc atgagacacg atcgcggcta
      661 ttttaatgtc tccaacggcg gcaaaatcca tgttaagggc accagccgtc tgacctattt
      721 gcagggaaat gtcagtggtg aaggtagcca ggtaaattcc gaaaccttct ttatgggcgt
      781 ttacggcagt tacggtggta atcagtacct gtcagttaat aacggcggtg aagttaatgc
      841 caggaagcaa attagcctgg gctattatga tcaagtctcc gatacaacac ttgctgtttc
      901 ggaaggtggt aaaatttctg cgcctactat tagtttaagc accaactctg agttagcgtt
      961 aggggcacag gaaggaagcg cagcgaaggc agcagggatt attgatgccg aaaaaattga
     1021 gtttgtgtgg gcaaagacat ccgagaagaa aatcacctta aaccacacgg ataaagacgc
     1081 gactatttcc gcggatattg tcagtggcag cgagggcctg ggctatatca atgcgctcaa
     1141 tggcacgact tacttaaccg gtgataactc tgcctttagt ggtaaagtca aaattgagca
     1201 aaatggcgct ttagggatca cccaaaatat aggtacagca gagatcaaca accgcgggaa
     1261 attacacctg aaggctgacg atagcatgac ctttgccaat aagatctctg gcaacggtac
     1321 aataagtatc gacagtggga cggtggagtt gaccggcaat aactatgcat tcagcggata
     1381 tattgatgtt gcttctggtg ctgtcgctgt tatttctgaa gacaagaata tcggtcgtgc
     1441 agagctggat gtcgatggca aattgcaaat taatgccaac aaagattggg tatttgataa
     1501 cgatcttgaa ggtagaggca ttgttgaaat aaacatgggg aatcacgaat tctccttcga
     1561 tgagtttgct tatacagact ggttccaggg ttcactggcg ttccagaaca cgacatttaa
     1621 tctggaaaag aatgctgagt ttctgcagaa aggcgggatc actgcgggtc agggaagcct
     1681 ggtaacagtg ggtaagggcg ctcactccat tagcactttg ggattctccg gcggaaccgt
     1741 tgattttggt gccctgacag caggtgcaca gatgacagaa gggacggtca acgttagtaa
     1801 aacgctggat ttgcgcggcg agggtgtgat tcaggtttct gacagtgacg ttgtccgctc
     1861 agtatctcgt gatattgact ctgcgttatc gctcactgaa gtcgatgatg gtaacagcac
     1921 cattaagttg gttgatgcgc aaggtgcgga agttctgggc gatgcgggca atctgcaatt
     1981 gcaggataaa aatgggcaaa tcctctccag cagcgcccaa cgtgatattc agcagaatgg
     2041 gcaaaaagcg gccgtcggca cttacgacta tcgtctgacg agtggggtaa acaatgacgg
     2101 tctgtatatt ggttacggcc tgacccagct tgatttacac gctaccgaca gcgatgctct
     2161 ggtgctgagc tctaacggta aaagcgagaa tgccgccgat ctcagcgcaa agattaccgg
     2221 cagtggtgac ctggcattca gcagccagaa gggtcagacc gtatcgcttt ctaacaaaga
     2281 caacgactat accggtgtta ccgatctgcg cagtgggacg cttttgttga ataacgataa
     2341 cgtgttgggt aatacccatg aactgcgtct ggcggcagag actgaactgg acatgaatgg
     2401 tcacagccag accgtgggca cgctcaatgg cagcgccgat tcactgctga gcttaaatgg
     2461 cggcagtctg acggttacca acgggggcac ttcaaccggt tcgttaacgg ggagcggaga
     2521 gctgaatatt cagggcggca cgctggacat cgcgggcgat aacagcaacc tgacggcgaa
     2581 tgtgaacatt gctaattcgg ctaatgtcct ggtaagtcat gcgcagggat tgggtagcgc
     2641 aaacgttgag aacaacggta ccctggcgtt gaataatagc gctgaaaaaa gagcggctgc
     2701 gtctgtgaat tacgccctgg gcggcaatct gaccaacaac ggtacgctga tgaccggaat
     2761 gtcaggacag caagctggca atgtgttagt ggtgaagggg aactaccacg gtaataacgg
     2821 tcaactagta atgaatacgg tactgaatgg cgatgactca gtaaccgata aattggttgt
     2881 cgagggcgat actagcggca cgactgccgt tacggtgaat aacgctggcg gtacaggtgc
     2941 gaaaaccctt aacggtatcg aacttatcca tgtagacggt aagtctgagg gcgaatttgt
     3001 tcaggctggg cgtatcgttg cgggggcgta tgactacact ctcgcgcgtg gacaaggggc
     3061 aaatagtggt aactggtatc tgaccagcgg cagtgattct cctgaactgc agccggagcc
     3121 agacccgatg ccgaatccag agccaaaccc gaatccagag ccgaacccta acccgacacc
     3181 tacgccgggt ccggatctga atgtggataa tgacctgcga ccggaggcgg gtagctacat
     3241 tgcgaacctt gcagcagcga ataccatgtt caccacgcgt ctgcatgagc gtctgggtaa
     3301 tacgtactat accgacatgg tgacgggtga gcagaaacaa accactatgt ggatgcgcca
     3361 tgaaggtggt cataataaat ggcgtgatgg cagcggccag ctgaaaaccc aaagcaatcg
     3421 ctatgttctg caactgggag gcgatgtcgc gcagtggagc caaaacggca gcgaccgctg
     3481 gcatgttggg gtcatggcgg gatatggcaa cagcgacagc aaaaccattt cctcgcgaac
     3541 cggttatcgt gcaaaagcga gtgtgaacgg atatagcaca ggcctctatg ccacctggta
     3601 tgccgatgac gagtcgcgta atggcgcgta tctcgacagt tgggcgcagt acagctggtt
     3661 tgataacaca gtgaaagggg atgacttaca aagtgaatcc tataaatcaa aaggatttac
     3721 cgcttcactg gaagctggat acaaacacaa attagctgaa tttaatggca gccagggaac
     3781 gcgtaatgaa tggtatgttc agccgcaagc acaggttacc tggatgggag tcaaagccga
     3841 taagcaccgc gaaagcaacg gaaccctcgt tcatagcaac ggtgatggca atgttcaaac
     3901 ccgacttggc gtaaaaacct ggctgaagag ccaccataaa atggatgacg gtaaatcccg
     3961 cgagttccag ccgtttgtag aagtgaactg gctacataac agtaaggatt tcagcaccag
     4021 tatggatggc gtgtctgtca ctcaggatgg agcccgaaat attgctgaga taaaaaccgg
     4081 ggtggaagga cagctaaatg ccaacctgaa tgtctggggg aatgtgggcg ttcaggttgc
     4141 cgatagggga tataatgaca cctctgcaat ggttggcatt aagtggcaat tctgatctga
     4201 ataatgagta aaaggagaga gagccgaacg ggagataatt cggctctctg tgtcattttc
     4261 ttgcagccag ctttatccgt cagagtgaag actcgcagat ttagctggcg cacaagattt
     4321 aatcaaggtg tgttcaatgg cttcgcatga tttgagtgtt tttttggaag agttcggggc
     4381 aacggtgaat ttgacgttgc ctggtatcgt gtccgaaaaa gaacggcttt tactcaagct
     4441 gctaatggag ggaatgtcgg ttacagaaat ctcacagtac agaaatcgca gtgcaaagac
     4501 aatttcacat caaaagaagc agctgtatga aaaactaggc atccagagtg atattacctt
     4561 ctggcgggat atcttttttc aatatcatcc gcaagtcatt agtggcacgg gaaataaaaa
     4621 taatttttat atccctgata accgttgtca ccatatcgtc acgcctgaag ccatcagtct
     4681 ggcgttggaa aaccatgaat ttaaaccgtg gatccaaccg gttttctgcg cgcagactgg
     4741 ggtactgacg ggctgtgaag tgcttgtccg ctgggaacat ccacaaacgg gaatcatccc
     4801 gccggatcag tttattcctc tggcggagtc atccggtctt attgtcataa tgacccgcca
     4861 actgatgaaa cagactgcgg atattctgat gccggtaaaa catttgctgc cggataattt
     4921 ccatattggt atcaacgtct cggcgggttg ttttttggcc gcgggatttg aaaaagaatg
     4981 cctgaacctg gtcaagaaat taggaaacga taaaatcaaa ctggttctcg agctaacgga
     5041 acgtaaccct attccggtaa cgccagaagc cagagcgata tttgacagcc ttcatcagca
     5101 taacattacc tttgcgctgg atgactttgg tacgggttat gcgacctatc gttatctgca
     5161 ggcgtttccg gtcgatttta ttaagatcga taagtcattt gtgcaaatgg cgggtgtcga
     5221 cgaaatatcc ggtcatattg tggacaatat tgtcgaactg gcgcgtaagc ctggtctgag
     5281 tatcgtggcg gaaggggtag aaacccagga gcaggcggat ttaatgatcg gtaaaggcgt
     5341 tcactttttg cagggctatt tgtactctcc gccagtaccg ggtaataaat ttatctctga
     5401 atgggtaatg aaagcaggtg gttgatgtaa accgctattc acagcgcatc gggaggttgg
     5461 cagcgattaa ttctcccgat gcagtaaatc ctgatagatc cccgttaata tgccatttgg
     5521 cgcaaattgt tttttaatcc actgcgtcac ctgacccgta gcagaatgct gagtcgccag
     5581 taacatgcgt gaatcctggc gcgggttatg gatttgtcgg gtaaccagca gggattgcgc
     5641 catcgcctca cggaccatgt aatctggcaa aaaaacaatc ccttcaccta aaatctggca
     5701 ctgacatttg gtgttgaaat ctggcaccag aattgactcc tgcccgtgca gcaaccagcc
     5761 cactttttta ttaatcgtat gcgcggtgtc ctccaccata atattagggt agagacgcag
     5821 ttggctttcg gcgatggttc cggcacaaag gctaacgggt gatccggggc gatagcaaaa
     5881 gcccagcgaa tcgcgccaat ttcagtgtaa tcaataccgc cgccgtccag cagcgtgtcg
     5941 ggtgcgccga tagcgatatt tgcctgatta ttaataatcg catcccagac gccgttatac
     6001 acttcggtgg tgacggtaat ctggcaggta ggaaactgct ttttcagcac ctgtaataag
     6061 cgtgcggtgt gtttgggcgt ataaagcaac tgattaatac aaatgcgcac acgcgcttct
     6121 atgccctgcg aaatagtatc gatgctgcgt ttgatggcgt gaaaatcatt cagcaggtcg
     6181 gtagctttgc ggaaaaaata acgcccggac tccgttaact caatgctgcg ggtactgcga
     6241 gtgaacagca ccacatccag ccctgtttcc attcgcttga tggtgtagct aatggcggaa
     6301 gtggttaaac ccaactcttc tgcggcttta ctgaaactgc caaaacgcgc agcggtagta
     6361 aatgccaaca gattctcttc ggtaaaaatt gagttcatta aaccattccc gccattatct
     6421 aatatgaatt tctccaagca taactgtaga ggtatcaatt agagatgggt aatcattttt
     6481 gaataacttt taatacccgt cgcgtttagt cgtcgcttcg cttaattttt gtccgccata
     6541 ttctgaaatt atccccacgc agttacccgc cagaaaaccc agtaacgtca ccgtccagtt
     6601 catcccgctg gcaaaaaaca gcgtcatgcc gagaaagcca ccgggaataa atgacagtaa
     6661 ccagaaacgc ccttgccaga ccaccaccgc cgagaagggt acggtagtaa ttacgctcgc
     6721 ccagaacgcg gacagtccac ttacagaggc cagccaaccg gcagccagcg cggcgaaaaa
     6781 cgcccacacc atcccggaat aattaaccga cagacttctg ataaaccccg atttaccgcc
     6841 gcctgcggca taaaaactgc aaaaggcgac gaagccaatg gttcctaata attcctaata
     6901 attcccatcc ctgagagagc ccgacatgtg aagacactaa ctgccaaagc cctgcacaaa
     6961 taccgacggt gacacccgtt gccgtgagtc cgttcatctt gttcccctct ggttagacat
     7021 ggacgaaagc tactcttaca tgtgcagact atagagaaat aaaacctgat tgagtgaaag
     7081 ggtatcgggt caaagaaaca aatattgcac taccgcacac tgcgaaaaga ttgttgaaca
     7141 tcattcaaca aaaaggcgaa atacggcgaa ttaaatttaa gcgggttctt ttatcagatt
     7201 atcgttgcta ttctcaattc atcgaaaaca gccacaaaaa ccaggggaat gtgaatgagt
     7261 ataaaaaatc tacctgccga ctatttattg gctgcacaac agggtgatat cgataaagta
     7321 aaaacctgcc ttgcgctggg tgtcgatatt aatacctgcg atcgtcaggg gaaaacggca
     7381 attacgctgg caagtttata tcagcaatat gcttgcgttc aggcattaat tgatgccgga
     7441 gcggatatta ataagcaaga tcatacctgt ttaaatcctt ttttaattag ctgtctgaac
     7501 ggtgatttaa cgctgctacg aattatttta ccggctaaac cagaccttaa ttgcgtcacc
     7561 cgttttggcg gtgtcggcct gacgcctgcc tgtgaaaaag gccatttaag tattgtaaaa
     7621 gagcttttgg cgcatacgga aattaacgtt aaccagacca accatgttgg ctggacgcca
     7681 ctactggaag cgattgtgct taacgatggc gggattaaac agcaggcgat tgtgcagtta
     7741 ttgctggaac acggtgccag cccgcatctg accgataaat atggcaaaac gccactggaa
     7801 ctggcgcggg aacggggctt tgaagagatt gcgcagttac tgattgccgc aggtgcataa
     7861 accgggaggc ttgctatcaa cacaccagaa aaacggtgtg tgtgggcgct aactgcggat
     7921 gcggattttc tggcgcagcg agggcaagga caggttgaac aggtctttgc cagagcggta
     7981 aatatcgcac tcccggctcg ccagcagttg ctgacgctgc tttgtgaaga gtacgacaat
     8041 gcgccaaaca gttgtcggtt ggcactcact cactttgatg atctgttccg gcatggtgat
     8101 aaggttcagt ttgacgatca aggtattacg gttggtcaac atcttcatat agagatgagt
     8161 cgttgtcggc gttggctgtc cccaaccttg caaatgaccg ctgtgaattt tcgccttatc
     8221 gcctggcaac agtggcacga catcattcat cagcacctgg gggaaaatga aaccctgttt
     8281 aattatcgcg gcgataatcc gttttatcag gcgttaaata aagaattaca tattaaacga
     8341 cgggcagtta tacaggcagt aatcgataaa caaaatatcg cctcagcggt cgccagtatg
     8401 atgggcttag ggattggcct tacgccatca gccgatgatt atttaacagg tctggcactt
     8461 attttattta tttccgggca tccggcggga aaatacaaag aggaatttta tctcggcttg
     8521 caacgcggca gaaataatac cacgttatta agtgccataa cgctggaagc cgcattacaa
     8581 caacgctgcc gggaaaatat tcatcgtttt attcacaaca ttatttatga catccctggg
     8641 aacgcaactc aggcaataga aaaaattaaa catattggct ccagttccgg ctgcgacatg
     8701 ctgtatggca tggccgatgg ttgtgcgctg agccaaacct acggagggaa ttatgtcagt
     8761 taaaatagtc attaaaccga atacctattt tgattctgtc tcgctgatgt ctatctccac
     8821 gcgtgcaaat aaactcgacg gcgtcgagca ggcatttgtg gcgatggcga ccgaaatgaa
     8881 caaaggtgtg ctgaaaaatt taggactgct gacgccggag ctggagcagg cgaaaaacgg
     8941 cgacctgatg attgtcatca atggtaaatc gggggcggac aacgagcagt tgctggtgga
     9001 gattgaagaa ctgttcaaca ccaaagcgca aagcggctcg cacgaggcgc gttacgccac
     9061 tattgccagc gccaaaaagc atatcccgga aagtaacctg gcggtgattt cggtcaacgg
     9121 tctgtttgcc gctcgcgaag cgcgtcaggc gctgcaaaac gatctcaacg tgatgctgtt
     9181 ttccgataac gtctcagttg aagatgaact ggcgctcaag caactggccc acgaaaaagg
     9241 gctgctgatg atggggccag actgcggcac ggcgattatc aacggtgcgg cgctctgctt
     9301 tggtaacgcc gtgcgtcgcg gcaacatcgg tattgttggc gcatccggca ccggcagtca
     9361 ggagttgagc gtccgcattc atgaatttgg cggcggcgtt tcgcaactga ttggcaccgg
     9421 cgggcgcgac ctgagcgaga aaatcggcgg cctgatgatg ctcgacgcca tcgggatgct
     9481 ggaaaacgat ccgcaaactg aaatcattgc gcttatctcc aaaccgcctg cgcctgcggt
     9541 ggcccgcaaa gtgctggaac gtgcgcgcgc ctgccgcaag ccggtggtcg tttgcttcct
     9601 cggtcgtggc gaaacgccag tggatgagca ggggctacag tttgcccgcg gcaccaaaga
     9661 ggcagcgcta aaagcggtga tgctctccgg cgtgaaacaa gaacatctcg acctgcatac
     9721 cctgaaccag ccgttgatta ctgacgtgcg cgctcgcctg aagccgcagc agaaatacat
     9781 tcgtggcctg ttctgcggtg gcacgctgtg cgatgaaacg atgttcgcgg tgatggaaaa
     9841 acacagcgat gtctacagca acatccagcc cgatccggaa ttccgcctga aggatatcaa
     9901 ccgcagcatc aaacacacct tcctcgactt tggcgatgac gacttcacca acggcaagcc
     9961 gcacccgatg attgacccta ccaaccgcat cagccgcttg atcgaagagg cgcgcgatcc
    10021 agaagtggcg gtgatcgtga tggattttgt gctcggattt ggatcgcatg aagatccggt
    10081 cggttccacc atcgaggcga tcaaagaagc gaaagcgatc gccgctgccg aaggacgcga
    10141 gttgatcatt ctcgcctatg tgctgggtac cgatcttgat acgccatcgt tagaacaaca
    10201 aagccagatg ctgcttgatg ccggcgtgat tctggcgagc agcagcacca ataccggatt
    10261 gctggcgcgt gaatttatct gcaaagggga ggaagcctga tgagccagtc actgtttagc
    10321 caaccattga acgttattaa cgtcggcatc gccatgttta gcgatgacct gaaaaagcag
    10381 catgtagaag tgactcaatt cgactggacg ccgccggggc agggcaatat gcaggtggtg
    10441 caggcgctgg ataacattgc cgattcgccg ctggcagaca aaatcgccgc cgctaaccag
    10501 caggcgctgg agcgcattat ccagtcgcat ccggtgctga ttggttttga tcaggcgatt
    10561 aacgtggtgc cgggcatgac gccgaaaacc attcttcacg ccgggccgcc gatcacctgg
    10621 gaaaaaatgt gcggcgcgat gaaaggcgcg gtcaccggag cgctggtgtt cgaaggactg
    10681 gcgaaagatc tcgacgaggc ggctgaactg gcggcttccg gggagatcac cttctcgccg
    10741 tgtcacgagc acgactgcgt gggatcgatg gcgggtgtga cctcggcctc gatgtttatg
    10801 cacatcgtaa aaaacaaaac ctacggcaac atcgcttata ccaacatgag cgagcagatg
    10861 gcgaagattt tgcgtatggg cgctaacgac cagagcgtga ttgaccgcct gaactggatg
    10921 cgtgatgtgc agggaccaat actgcgcgac gcgatgaaaa ttatcggcga aatcgatctg
    10981 cgcttaatgc tggcgcaggc gctgcatatg ggcgatgagt gccataaccg caataacgcc
    11041 gggacgacgc tgctgattca ggcgctgacg ccggggatta tccaggcggg ctattccgtc
    11101 gcgcaacagc gcgaagtgtt tgagtttgtt gccagtagcg attacttctc cggcccgacg
    11161 tggatggcga tgtgtaaagc ggcgatggat gcggcgcacg gcatcgaata cagcaccgtc
    11221 gttaccacca tggcgcgtaa cggcgtcgag ttcggcctgc gggtgagcgg cctgccgggg
    11281 cagtggttta ccggcccagc gcagcaggtc atcggcccga tgtttgctgg ttataagccg
    11341 gaagattcgg ggctggatat cggcgacagc gccatcacag aaacctacgg tattggcggg
    11401 tttgccatgg cgaccgcgcc tgcaatcgtc gcgctggtgg ggggcacggt ggaagaggct
    11461 atcgatttct cccgtcagat gcgcgaaatc acccttggcg aaaaccccaa cgtcaccatt
    11521 ccgctgctcg gttttatggg cgtgccgtcg gcagtcgaca tcacccgcgt gggtagcagc
    11581 ggcattctgc cggtgatcaa caccgccatc gcccataaag atgcgggcgt cggcatgatt
    11641 ggcgcgggca ttgtgcatcc accattcgcc tgcttcgaga aagccattct tggctggtgc
    11701 gaacgttacg gcgtttgacc tacctgttca t
//
LOCUS       DAZFTN010000137        11523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFTN010000137 DAZFTN010000000
VERSION     DAZFTN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..140
                     /locus_tag="ACHKV2_004239"
     CDS             <1..140
                     /locus_tag="ACHKV2_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFO6942008.1"
                     /translation="YPRKTSLYEKLSIPFIYLLFQWSFPHLHSDGMTVVIRPLQTHHT
                     N"
     gene            469..1536
                     /gene="pilL"
                     /locus_tag="ACHKV2_004240"
     CDS             469..1536
                     /gene="pilL"
                     /locus_tag="ACHKV2_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFO6942009.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSTPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESSTTANSQN
                     KPRNPFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1536..1973
                     /gene="pilM"
                     /locus_tag="ACHKV2_004241"
     CDS             1536..1973
                     /gene="pilM"
                     /locus_tag="ACHKV2_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO6942010.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RLKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1987..3669
                     /locus_tag="ACHKV2_004242"
     CDS             1987..3669
                     /locus_tag="ACHKV2_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO6942011.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3662..4957
                     /gene="pilO2"
                     /locus_tag="ACHKV2_004243"
     CDS             3662..4957
                     /gene="pilO2"
                     /locus_tag="ACHKV2_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO6942012.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTA
                     EEIAARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRLLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4944..5396
                     /gene="pilP"
                     /locus_tag="ACHKV2_004244"
     CDS             4944..5396
                     /gene="pilP"
                     /locus_tag="ACHKV2_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO6942013.1"
                     /translation="MRPGKSLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVNLSSETVSGFSGMTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5407..6960
                     /locus_tag="ACHKV2_004245"
     CDS             5407..6960
                     /locus_tag="ACHKV2_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO6942014.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLNLY
                     PDIIPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     VMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSED
                     DRAYLERHCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDA"
     gene            6973..8058
                     /locus_tag="ACHKV2_004246"
     CDS             6973..8058
                     /locus_tag="ACHKV2_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO6942015.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPVLSKI
                     SSPDSWSGALGFLYGLSLFVDNYGAICAVLFAVVTGVISWSLKNWKSRSLADNIMPWS
                     IYKDIQGAAFLLNMAALLKAKMTTLNSLNVLQDFASPWLSTRLDSIIYRVRQGDHLGL
                     ALRQCGYQFPSREAANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLI
                     FLVMSLLLLVMAVVDIQSIGDNSMGNL"
     gene            8075..8689
                     /locus_tag="ACHKV2_004247"
     CDS             8075..8689
                     /locus_tag="ACHKV2_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6942016.1"
                     /translation="MLIQNIDVNALKKNEKETSHDKGWGIMEQGAIALVVIIVLALVF
                     GGLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGAKMTGALIQMGGVPKTM
                     TVRGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTG
                     LTNGITLNSTTHNDGKVTTEEASAQCTADNGSTGTNKLIFTING"
     gene            8699..9259
                     /locus_tag="ACHKV2_004248"
     CDS             8699..9259
                     /locus_tag="ACHKV2_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6942017.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIK
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKVWRIYRDMKGICLPGQGGRQCRQS"
     gene            9244..9900
                     /locus_tag="ACHKV2_004249"
     CDS             9244..9900
                     /locus_tag="ACHKV2_004249"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO6942018.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWY
                     KTHTAVLSGIWLYSIAVALIILSPSQLLYRIHAVLILSFMLQMAVTDALTGLLPGTFT
                     RRFLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI
                     "
     gene            9900..11192
                     /locus_tag="ACHKV2_004250"
     CDS             9900..11192
                     /locus_tag="ACHKV2_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO6942019.1"
                     /translation="MKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGWQAEA
                     RLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAILSCQSGTWGTIGGKLKVTQLSTTGYLGQFDFCAIARM
                     GNAEDAHYCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
     gene            complement(11189..>11523)
                     /locus_tag="ACHKV2_004251"
     CDS             complement(11189..>11523)
                     /locus_tag="ACHKV2_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin, shufflon protein A"
                     /protein_id="HFO6942020.1"
                     /translation="TSGSLNGSYTNLGSHRGSFSGRNSGGSTLFIYASGGNGGSAGGA
                     CANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITSYPTENTSCGAGV
                     FSVFGYQT"
BASE COUNT         3327 a         2746 c         2616 g         2834 t
ORIGIN      
        1 catacccacg gaaaacaagt ctgtacgaaa aattatcaat tccttttata tatctcttgt
       61 ttcagtggtc tttcccgcat ttgcattcgg acggaatgac agtcgtcatt cgtcctctcc
      121 agacccacca caccaattaa aaaatacatt tagatataaa aagaatgctc ataagcagga
      181 tttttatttt cacaaaaaat cccaaaacac tggcttaatt ggctatccac aacctcacct
      241 ttaaaataac atgcaacatt aaaattcaaa agatagaata atgaaaatac taatcaacaa
      301 tattaaagac ccaagcagac ttctgaatgc gactaaaacg ggacattcag gagtaattct
      361 ctcaataccg gtatacatta cccatccaat tgcacataaa ataatgaacc cttacttaaa
      421 gtccgcatta actaacaaac acgtatatac gaacacggag tctccgttat gaaaaaaaac
      481 tatctgacac ttatactgcc gtcattattg tccggctgtg ccggaatttc cggcggcaat
      541 aacgtggcac catcagatac gcctgctctg gtttttgttg acggacaaat cagtgagagt
      601 gctggcgtcc tggcacttac gcaacaacga ctttctccac ctaaaataac tccgcccccg
      661 agagtcagcg tgccagagac agtaaaaaca gcacagccag taagcgaatc ccgaccgtta
      721 ccttcaccaa caataagtca gaatttctcc cggttaatca ttacaggcaa gcctggtagc
      781 acccctgtaa cactatcgcc gacggcccgt aaccggaccg tcagtcagtg gataaaaaca
      841 ttactaccgg aaggatggaa attcagacaa gagaatgcag ccacgcccaa actgaatacc
      901 aaactggttt catggtctgc caatgatcag tggacccgtt ctctgaaccg gttgctggaa
      961 gaacaacacc tctggggcca tctggactgg gataataaaa cgttgaccgt cacaacatcg
     1021 gctgctgcac cggcacagga cacggcaacc actatctcgc cagcagccat ggtaaccaca
     1081 tccacttccc cggagtcttc aacgacagca aatagccaga acaaaccacg aaacccattc
     1141 aggggaaaca gcgtatctcc gtctacgccc gcagcaatcc cgactccgac cggctctaca
     1201 gtcaagtcta tcccactcat gaccgggaca cctgtcaaac cggtatctca gggtaaagag
     1261 tggcgtgcgc cggcaggcac tacattaagg gaaaacatta ttaagtgggc agaagaaaca
     1321 aaatgcgaaa gcatggcatc aacgcactgg atggtcatat ggccactttc tgtaaccgat
     1381 taccgtcttg atgcgccttt gatgttccgg ggatcatttg aatcagccat ggagcaggta
     1441 tttgagttat atcggactgc gcaaaaacct ttatacgcac aggcttctcg catgcagtgc
     1501 atcattacag tttctgatac aagggatggt cgctaatggg atggctggtc atggccgtcg
     1561 gcctgaccat tctgattatc actggtagct accagaatca gaagatgtct gaaaccacaa
     1621 acgcccagca atatgcaagt gcgtctgtat gggcaagtca gatactaatg attgctaaca
     1681 gaatcaatga tatcagatac gtatcaggcc agcaggacgg agtaatttcc tcagataaac
     1741 tggctctccc tgtcactccg gactcccgaa ttaaacatca gttacaacag gggcgtctgt
     1801 gggtatggat gcctgaacaa ccaggacttg ttgaaacttt gcgtttaaaa agtcgtggtt
     1861 ctgcactaat agggatcttc caaaacgggc aactcacctg gctatcagga actgcaaccg
     1921 gtctgacacc cccggcaggc attactgctg gttctgtcgt ctacgttaac taatctggat
     1981 atattcatga aaaaatcaca ccagcgttca atgaagctgg cggtgctccc ctgcatgatc
     2041 gccgtcgctc tttccatctc cggatgtact ttcagcgaaa tcaacaaaat gcagaaaaaa
     2101 gcacaggaag actcagcaca tgcacgggaa aaggtatcag ccctttcggc ccgtaaatcg
     2161 caggctctta cctggctcga taatcaatgg ataaaccctg ttccggtcgc tcaggtatca
     2221 agagagaaaa aacaaacagc tccagcctgc tacatcacgc aggcaagaaa aggagagatc
     2281 actctgcagg aactggggca acgtattact gccgtatgcg gcatccctgt gatcatcacg
     2341 cctgacgcag ccaattcaac tcttgaagga ggcgctaccc gccagatgac aggaacacta
     2401 ccagcaccag atgaaaatgg acgtctaccg ttaagcagcc tgggcagcac aacaatgact
     2461 acctccactc agccattaac gctgaataac ctcatgtggc agggagatat caatggtctt
     2521 ctggatctga tggccagccg gagtggtctg tactggcgca tggataatgg tcggattgta
     2581 ttctatctga ctgaaaccag aacgtatcca cttcatatgc tgaacaccaa aaccagcagc
     2641 agttccagtg tcagctctgg ctcaacaagc acaatggggg caacaggagg ccaggataac
     2701 tcagcatccg gtgatgcaac gtcctctcag agcacaaccg ttggtcagga atacgatctg
     2761 tatgaagata tccggaaaac tattgaagca atgctgacac cagaaaaagg ccgttactgg
     2821 ttatctgcat cgagctcaac gctgacagtc actgatactc cagctgtcca ggaagccgtc
     2881 gcacgatatg tagacgaaca aaacagtatt atgaaccgcc aggtagccct gaacgtacag
     2941 gttctgagcg tcagcaatac cagaaacgaa cagttcggtc tggactggaa ccttgtttat
     3001 aaatcgctac attccgccgg agcaacgttg aacaatgcaa gcggagattt tacaggcgct
     3061 acatctgcag gcgtatcaat tctggataca gcaacaggga atgccgccaa attcagcggt
     3121 tccagccttc tgattaaagc actgagtgaa caaggcgatg tcagcgttgt aacttcacaa
     3181 gaaagcactg tcacaaacct gacgccggta cctatccaga tggcagatca gacagtttac
     3241 gtcgcccagt cagcaacaac tacgactacg gatgtaggag caacaacaac attaacaccg
     3301 ggcatgatca ccactggatt caatatgacc ctgctgcctt taattcagaa aacaggcaat
     3361 ctccagttgc agatgaactt taatctgtct gatcccccaa ctatccgtag ctttacgtca
     3421 aaagacggaa acagttacat cgaaatgcca tataccaaac tgcgttcact gagccagaag
     3481 gtcaatctga aagaagggca atcacttgtc gttactggtt tcgatcagaa caatacgacg
     3541 acaagtaaag ccggtacatt tacgccagca aatccattat ttggtggttc acaaaccggg
     3601 aaaaatgaac gcagcacgct tgtgatcatc attaccccga ctttcccgtc aggaggcaat
     3661 aatggctgat gaagatatca acccggtagt gcttctcgca gatcctaaag taaatcatcg
     3721 cgtgtgggct gcctgtctta aatggactcc tgtcgtaaaa aaacagcgcg tcccctcaca
     3781 tcataaacac aagtctcatg taaaacccag acggcttacc tccctgaaag tgactgtagg
     3841 cagcagaaat tcccgtggaa agatcagccg ccttaccggc acaggaatac tgaccaggcc
     3901 agaacgcaat cattattttt ccctggcact ggctttctgc tcatgggtca gaaatggata
     3961 cggtgtcttc cggtacagcg ataaggaact cttattcctt gcttccatta atggccagcc
     4021 tgccgttatg gctgacttat caggaaatga tgccgatgtt gcccaaaagg tcagcctttt
     4081 tctggcgatg aacgaagagc cccccgaaaa atggcaggtt gtatcgtctc tggaacatcc
     4141 tgataactgg gaaagtatta ttacccggtt atcatcagca gacctccgcc gttgcaaact
     4201 tactgtcggc aatcgcagca aattcacact gcctgccgta ctgtttttag tagcagccgg
     4261 cgcaggcaca gtattctgga tgactcagcc agagcctgat gtcggaccga ccgccgagga
     4321 gatagctgca cgggcaagat tacagtttaa aaagcccgaa ccaccaccgg agctcccgca
     4381 tccatgggcg tcacaacctg taatcagtga ttttctgaaa gcctgcgcgg accttcgaaa
     4441 accatcaccg gtcgcgctag aaggctggaa acttaccggt ggaacatgta ctcccgagac
     4501 attcacactg atttacgaac gacagccagg aggaactatt gaaggctttc tggcaagaag
     4561 caaagaaatc tttaatgtta ttcctgactt taaccttaaa gatggtgcac gcttggcttc
     4621 tgtaacccgg ctactgcctt ccctgccccg cagggatgag gcagtccctg ccccctcaga
     4681 acaactgatg cgggtgttca cgtggtttca gaaaaaacag ttaacgccgg ccattaatga
     4741 aatagccata ccggaaccat taccaggtaa cgatggtgaa ccagcccctg ttcagaaatg
     4801 gaaagaatat cagttcagtt tatcaacgcc tgtaaatcct gatgagttgt tccctttatt
     4861 tcaggatacc ggcgttcgtc tgagcaatat ccattttgaa ttaaacggcg gtactttcag
     4921 ttattcctca gaaggtcata tttatgcgtc caggtaaatc actcattatt ccatccatat
     4981 ttttcttctc cggtttttca tttgcaacaa cacaacctct tgtcacaata ggagaacttg
     5041 aagcacaaca aaaccggaat attcttttac aggcaaaagt tcagggcgca cagctacaga
     5101 aacaactcga agaatcagat gttaatctgt catctgaaac tgtttcaggt ttctctggta
     5161 tgactgcctc tttgccatca gtttcagagc agccgacatc taaaaacaga aaggaattgc
     5221 ccgttattat ggaaataaac ggcaaagata aacgtcttca tgccgttctt cgcatggctg
     5281 atggacgtca gacaagcgtc accactggta gccagcttcc gggtacatcc gtcacggtga
     5341 agtcgatctc attgtctggc gtcacgttaa gtgatggcac cacccttact ttctgagtga
     5401 tgtgatatgg acaaatataa tttgaaggat gctctccttt ttatatcccg gggagacacc
     5461 catgaaattc tcatcgaaac aaatcagcga acaagacctg acgtacagag taatctccag
     5521 gaactgctaa acttataccc tgacatcatc ccaaaagtag tctcgctgtc tgagttacag
     5581 gaagccggtc aggatgatga aaataaaggt ccgaaagaac ggattattca tctgaaagat
     5641 cttgcagatg tcagtgaaag tgagaagaaa gttctctcct attttgaaac ggccagaaaa
     5701 cttggtgctt cagatattca ttttttaatt tcagagtcta ttttcaaagt tcgcatgaga
     5761 atattcggtg aactgcaaac tgtagatgaa gatcaacctg cgttgggtta ttcactttgc
     5821 gcaacagcca ttctgtcaat ggctgatgtg actgaaacca gtttcttccc tcaaagggaa
     5881 caggatgcgc gcctgtcccc acaattaatg cgaaaaatag gaatatttgg tgcacgatac
     5941 agtcacagac ctacgggaga tggtctgatt gcagttatgc gcctgatacc cgatgacgga
     6001 gacaaagttc ccacgtttaa gcaactgggc tttataccgg agcaaatccg gttgctgaac
     6061 gtcatgctgc gccgcccgga agggaaaata gttctgtctg gtccaacagg ttcaggtaaa
     6121 tcaacaacgt tacgcagtgc ctgccgtgta tatcttgacg ataatcaggg gcgacacctg
     6181 ctgacgatcg aagatccgct ggaaggacag attctggggg caacacaaac cccgattatc
     6241 tgtgacaaat ccgacgaaga ggccgtcaaa ctggcatgga gtcgtgctat ttcatcggca
     6301 atgcgacttg accccgatgc catcatggaa ggggaaatgc gcgatcttgt ctcgatgatg
     6361 tcaacaacct acgcggctca gaccggtcac atcgttctga cgacattaca tacaaactca
     6421 gctctgggta taccggagcg gatgattacg atgggtatga atgccgatct tatttgcgat
     6481 gcgcaattac tgattgggat gatcagccag cgtcttgtac caactctgtg tccctcctgc
     6541 cgtattccat gggaaaagcg agtacctgaa cttagcgaag acgaccgcgc ttaccttgag
     6601 cggcactgca ataaagattc actctgcagt acagataata tctggttccg aaatcctcac
     6661 ggatgctccg actgtaatca cgacgtaatt attaacggca gaaagcgcgg tgaaataggt
     6721 aaaggcctca ccggcaggac cgtaattgct gaagtgatag aacctgataa tcgcttattt
     6781 cagattctga aaacacgcgg caaagttgca gcacgtaaat actggcttga aaacatgcat
     6841 ggcatcagtc gtgtggagca tctgcttcgc cgtatcaatg aaggcctggt ggaccctctt
     6901 gaagccgaca gaatcatccc tctggacgaa gacgaacgcc tcagcatcga tgatgcctaa
     6961 caggagaaca acatgcgtga aatgaatttc agtcaacggc tgagacgttt cattgtcaga
     7021 aaaacgttca gcgcccccta cagggtgcag ttttacgaag ctctgcgttt tctgctggaa
     7081 aataaacagc cattaaagac cgcactggaa cagatgcggg atgcctggac agactttgga
     7141 cgaaaatggc atccctttgc tgaactggcc acagactgta ttgagtctct tcgtgaaaac
     7201 agtggcgaaa actctctgga atatactctt ggtttatggg ttccccagga agaggctgca
     7261 gtaatcagtg ccggaataag aagtggttcc attgtggatg cattacaatt tgccaccaca
     7321 cttacagatg ccaaaaagca actccatcag gccatatggc aaatggctat ttacccggtg
     7381 gggttgttaa tcatgatgac ggggacatta tatgttctta atacagagct aatacctgta
     7441 ctgagcaaaa taagctcccc tgattcatgg agtggtgctc ttggttttct ctatggatta
     7501 tctttgtttg tcgataatta cggcgctata tgtgccgttc tttttgcagt ggtcacaggt
     7561 gtaatatcat ggtctctgaa aaactggaaa agccgctctt tggctgacaa tataatgcca
     7621 tggagtattt ataaggatat acagggggca gcattcctcc tgaatatggc tgcacttctg
     7681 aaagccaaaa tgacaacgct caactcactg aacgttcttc aggactttgc atcaccatgg
     7741 ttaagcacac gccttgacag cattatttac cgcgtacgcc agggggatca ccttgggctg
     7801 gctttgcgtc agtgcggtta tcagttccca tccagggaag cagccaactt cctgtcttta
     7861 ttacagggag atggcgcaac ggagctaatc ggcaattatg gccaacggtg gcttgtacaa
     7921 acgctggagc gagtaaaaaa acgagcagct gttgtgcgtt taattatgct gatatttctt
     7981 gtcatgtcac tcctgttact ggtcatggcc gttgtagaca ttcagtctat tggcgacaac
     8041 agtatgggta atctttaaat aaaacgaggt atttatgtta atccaaaata ttgatgttaa
     8101 cgctctgaag aaaaatgaga aagaaacgtc tcatgacaaa ggctggggta ttatggaaca
     8161 gggtgcaata gcacttgttg taattatcgt acttgccctt gtttttggtg gattgtatat
     8221 gttgcgttca cgaaccggtg tggcaaatga aagttcgaac atacagacaa tcatcacaag
     8281 tactcagggg ctgctaaaag gtagcgatgg atatacgttt accagtggag ccaaaatgac
     8341 gggtgctctc attcagatgg gtggcgtacc caagaccatg acagtgcgtg gtactccatc
     8401 atcaggtaca gcaacgctct ataactcatg gggcggtgcc gtaactgttg ctccggcttc
     8461 aacatcaggt ttcaacaatg gttttaccgt cacctatgaa aaagtcccac aggatgcctg
     8521 tatccagatt gccacacaaa tcagtcgtac tggtttaact aacggtatta ccctgaacag
     8581 tacgactcac aatgatggaa aagtcactac tgaagaagcc agtgcgcaat gcacagctga
     8641 taatggcagc accggtacaa acaaactaat cttcaccatc aatggctaag gagttttaat
     8701 gtccgggggt aaagtgcccc tcagacttat ttcattaatc ctgctttgtt ctcttattta
     8761 tcccgcccga gcttcataca tcgcccccaa acaaatctgg actaacaagt gggacaattg
     8821 ctttgccgcc gccggcgcac gctatcaaat tgaaccatta ttgttgaagg ccatttctgc
     8881 cggggaatct tcattgaagc cgggcgcaat taacataaat aaggacagaa aaacaggcaa
     8941 agccagtagt acggattatg ggttgatgca aataaactca acccatattc caaagcttat
     9001 aaagatgggc gtaatcaaaa agtctgagga tttaattacc aaaccatgtc ttaatatcca
     9061 tataggaagc tggatactgg caagacattt tcagatatgt ggcgtcagct ggaattgcct
     9121 tggatcatac aatgccggat tccgtaagga cagacatgaa acaagagagc aatatgctaa
     9181 taaagtatgg agaatttatc gtgacatgaa agggatatgc cttcccgggc agggaggcag
     9241 acaatgcaga caatcgtagg cataagtctc tcccctgttt atatcctccc attgatgttc
     9301 atattttcca ttattggacg aactctgtta ttcagagttc gttatttttt atctgataca
     9361 gaacatctct ggtataagac acacactgca gttctgtcag gaatatggct atatagtata
     9421 gctgtggcat taattatttt atcgccatcc caacttctct accgtattca tgctgttctt
     9481 attctttcgt tcatgctgca aatggcagta acggatgcac taacaggcct cttacctggt
     9541 acattcacca ggcgtttttt aatcgctgga atattatcac aaatcacaac tgatatatgg
     9601 tggctccgtg caacagaaat tgtctctgcc gcaattgtct tattctgtct gcataaactg
     9661 gttaaccgcc atcgtcttaa tattggcaca ggagacctgt ggcttattgc aggtattacc
     9721 gcatggtcag ggctatataa cgccatatgg agtgtgttgc tgggcgcagg aggatttgtc
     9781 ttatggcatt caacatggtg cataaaagga cataaagaag gcccactggg tccatggctg
     9841 tgtcttggcc atgtcctttt gctactggat aatctttatc aaccactatg ggtaatctga
     9901 tgaaaaaata tgaccgaggc tgggcatcgc tggaaattgg cgcggcttta ttgattgtta
     9961 tgatcatagt tgcctggggt gcaggcatat ggcaggacta tttaaaaacc aaaggttggc
    10021 aggctgaagc tcgtcttgtc agcaactggg ccagtgcagc ccgctcttat atagggaaaa
    10081 actacacaac tctccaggcc agtagtacca caacaacccc tgccgttatc acgacaacca
    10141 tgctgaaaaa taccggcttt ttatccagcg gctttactga gacaaacagc gaggggcagc
    10201 ggttacaggc atatgtagtt cgaaacaccc aaaacccgga attactacag gcaatggttg
    10261 tatccagtgg tggcacccct tatccagtga aagcacttat ccagatggct aaggatatta
    10321 ccactggtct tggtggatat atccaggacg gcaaaacagc cacaggtgca ttacgttcct
    10381 ggtcagtagc tttaagtaat tatggtgcca aaagcggtaa cgggcatatt gccgtactgt
    10441 tatcgacaga tgaacttagt ggtgcagcag aggacactga ccgtctttac agattccagg
    10501 tcaatggtcg ccctgactta aacaaaatgc acacggccat tgatatggga tcaaataacc
    10561 tgaataacgt tggggcagta aatgcccaga ctggtaattt cagcggcaat gtgaatggtg
    10621 taaatggcac tttcagcggt caggtaaaag gcaatagcgg aaactttgac gtaaatgtca
    10681 ccgctggcgg tgatatcaga agtaataatg gttggttaat tactcgtaac agcaaaggct
    10741 ggctcaatga aactcacggt ggcggatttt atatgtccga tggatcatgg gttcgaagtg
    10801 taaacaacaa gggcatctat accggcggtc aggtgaaagg cggtactgtt cgtgctgatg
    10861 gtcgccttta tactggtgaa tacttacaac tggaaagaac tgccgttgct ggcgcatcat
    10921 gttcgcctaa cggccttgta ggccgcgata atacaggggc aatactttcg tgccaatccg
    10981 gtacgtgggg gacaataggt ggaaaactca aagttactca gctttccacc acaggttatc
    11041 tggggcaatt cgacttctgt gccattgcca gaatgggcaa cgcagaggat gcccactact
    11101 gccaggtagt tgagagccca gcaggttcac gcaaatggta caaatacgag cataagacag
    11161 gttgtattgc gtcgtgtgta acgctcaatt aagtttgata tccaaatact gaaaataccc
    11221 cggcaccaca tgatgtattt tctgttggat aggatgttat ctgataggaa gtacctgcag
    11281 gtacagcaaa gctgataaag gctgtttttc catatgcagg gttattgctg gcattcacgc
    11341 taattagcgt cccaccaaca tatccctgca gtcgggatgt atttgcacat gcacctccgg
    11401 cagatcctcc attacctcca gatgcataaa taaacaatgt actgccccct gaattccgcc
    11461 ctgagaatga acctctatgt gaacctaagt ttgtgtaaga accattgagc gaaccagaag
    11521 tct
//
LOCUS       DAZFTN010000138        11523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFTN010000138 DAZFTN010000000
VERSION     DAZFTN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..210
                     /locus_tag="ACHKV2_004252"
     CDS             <1..210
                     /locus_tag="ACHKV2_004252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942021.1"
                     /translation="RKIRNHASEFTDLIILILIYFEKGIGIYAVFLKILIIGMDFRAR
                     KGFYMDTLLIISSSHHQQFTSLFRV"
     gene            303..2726
                     /gene="pgaA"
                     /locus_tag="ACHKV2_004253"
     CDS             303..2726
                     /gene="pgaA"
                     /locus_tag="ACHKV2_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO6942022.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2735..4753
                     /gene="pgaB"
                     /locus_tag="ACHKV2_004254"
     CDS             2735..4753
                     /gene="pgaB"
                     /locus_tag="ACHKV2_004254"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO6942023.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4746..6071
                     /gene="pgaC"
                     /locus_tag="ACHKV2_004255"
     CDS             4746..6071
                     /gene="pgaC"
                     /locus_tag="ACHKV2_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO6942024.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6073..6486
                     /gene="pgaD"
                     /locus_tag="ACHKV2_004256"
     CDS             6073..6486
                     /gene="pgaD"
                     /locus_tag="ACHKV2_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO6942025.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6536..7324)
                     /gene="phoH"
                     /locus_tag="ACHKV2_004257"
     CDS             complement(6536..7324)
                     /gene="phoH"
                     /locus_tag="ACHKV2_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO6942026.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7944..9215)
                     /gene="efeB"
                     /locus_tag="ACHKV2_004258"
     CDS             complement(7944..9215)
                     /gene="efeB"
                     /locus_tag="ACHKV2_004258"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO6942027.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9221..10348)
                     /gene="efeO"
                     /locus_tag="ACHKV2_004259"
     CDS             complement(9221..10348)
                     /gene="efeO"
                     /locus_tag="ACHKV2_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO6942028.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10406..11236)
                     /gene="efeU"
                     /locus_tag="ACHKV2_004260"
     CDS             complement(10406..11236)
                     /gene="efeU"
                     /locus_tag="ACHKV2_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO6942029.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3034 a         2830 c         2706 g         2953 t
ORIGIN      
        1 cgaaaaatcc gaaatcatgc atcggaattt actgatttaa ttattttaat cctaatttat
       61 tttgaaaaag gcatcgggat ttatgccgta ttcctgaaga tcctcatcat tggaatggat
      121 tttcgggcga gaaaaggatt ttatatggac actctgctca tcatttcttc ttctcatcat
      181 caacaattca cgtctctctt ccgcgtttaa taacggatta tgaggtgcaa aaatatcttt
      241 cttttcagtt acctgtaatt agatacagag agagattttg gcaatacatg gagtaataca
      301 ggatgtattc aagtagcaga aaaaggtgcc cgaaaaccaa atgggctttg aaacttctta
      361 ctgccgcatt tttagcagcg agtcccgcgg cgaagagtgc tgttaataac gcctatgatg
      421 cattgattat tgaagctcgc aagggtaata ctcagccagc tttgtcatgg tttgcactaa
      481 aatcagcact cagcaataac caaattgctg actggttaca gattgcctta tgggccgggc
      541 aagataaaca ggttattacc gtttacaacc gctaccgtca tcagcaatta ccagcgcgtg
      601 gttatgcagc tgtcgccgtc gcttatcgta acctgcaaca atggcaaaac tcgcttacac
      661 tgtggcaaaa ggcgctctct ctggagccgc aaaataagga ttatcaacgg ggacaaattt
      721 taaccctggc agatgctggt cactatgata ctgcgctggt taaacttaag cagcttaact
      781 ctggagcacc ggacaaagcc aatttactcg cagaagccta tatctataaa ctggcggggc
      841 gtcatcagga tgaattacgg gcgatgacag agtcattacc tgaaaatgca tctacgcaac
      901 aatatcccac agaatacgtg caggcattac gtaataatca acttgctgcc gcgattgacg
      961 atgccaattt aacgccagat attcgcgctg atattcatgc cgaactggtc agactgtcgt
     1021 ttatgcctac gcgcagtgaa agtgaacgtt atgccattgc cgatcgcgcc ctcgcccaat
     1081 acgctgcatt agaaattctg tggcacgata acccagaccg tactgcccag taccagcgta
     1141 ttcaggttga tcatcttggc gcgttattaa ctcgcgatcg ttataaagac gttatttctc
     1201 actatcagcg attaaaaaag acggggcaaa ttattccgcc ctgggggcaa tattgggttg
     1261 catcggctta tctcaaagat catcagccga aaaaagcaca gtcaataatg accgagctct
     1321 tttatcacaa ggagaccatt gccccggatt tatccgatga agaacttgcg gatctctttt
     1381 acagccacct ggagagtgaa aattatccgg gcgcgctaac tgtcacccaa cataccatta
     1441 atacttcgcc gcctttcctt cggttaatgg gcacgcctac gagcatcccg aatgatacct
     1501 ggttacaggg gcattcgttt ctctcaaccg tagcaaaata tagtaatgat cttcctcagg
     1561 ctgaaatgac agccagagag cttgcttata acgcaccagg aaatcaggga ctgcgcattg
     1621 attacgcgag tgtgttacaa gcccgcggtt ggcctcgtgc agcagaaaat gaattaaaaa
     1681 aagcagaagt gatcgagcca cgtaatatta atctggaggt tgaacaagcc tggacagcat
     1741 taacgttaca agaatggcag caggcagctg tcttaacgca cgatgttgtc gaacgtgaac
     1801 cgcaagatcc cggcgttgta cgattaaaac gtgcggttga tgtacataat cttgcagagc
     1861 ttcgtatcgc tggctcaaca ggaattgatg ccgaaggccc ggatagtggt aaacatgatg
     1921 tcgacttaac caccatcgtt tattcaccac cgctgaagga taactggcgc ggttttgctg
     1981 gattcggtta tgccgatgga caatttagcg aaggaaaagg gattgttcgc gactggcttg
     2041 cgggtgttga gtggcggtca cgtaatatct ggctcgaggc agagtacgct gaacgcgttt
     2101 tcaatcatga gcataaaccc ggcgcgcgcc tgtctggctg gtatgatttt aatgataact
     2161 ggcgtattgg ttcgcaactg gaacgcctct ctcaccgcgt tccattacgg gcaatgaaaa
     2221 atggtgttac aggcaacagt gctcaggctt atgttcgctg gtatcaaaat gagcggcgta
     2281 agtacggtgt ctcctgggct ttcactgatt tttccgacag taaccagcgt catgaagtct
     2341 cacttgaggg tcaggaacgc atctggtctt caccatattt gattgtcgat ttcctaccca
     2401 gtctgtatta cgaacaaaat acagaacacg ataccccata ctacaaccct ataaaaacgt
     2461 tcgatattgt tccggcattt gaggcaagcc atttgttatg gcgaagctat gaaaatagct
     2521 gggagcaaat attcagcgca ggtgttggtg cctcctggca aaaacattat ggcacggatg
     2581 tcgtcaccca actcggctat gggcaacgca ttagctggaa tgacgtgatt gatgctggcg
     2641 caacgctacg ctgggaaaaa cgaccttatg acggtgacag agaacacaac ttatacgttg
     2701 aattcgatat gacattcaga ttttaaggat aaatatgtta cgtaatggaa ataaatatct
     2761 cctgatgctg gtgagtataa ttatgctcac cgcgtgcatt agccagtcaa gaacatcatt
     2821 tataccgcca caggatcgca aatctttact cgccgagcaa ccgtggccgc ataatggttt
     2881 tgtagcgatt tcatggcata acgttgaaga cgaagctgcc gaccagcgtt ttatgtcagt
     2941 gcggacatca gcactgcgtg aacaatttgc ctggctgcgc gagaacggtt atcaaccggt
     3001 cagtattgct caaattcgtg aagcacatcg aggaggaaaa ccgctaccgg aaaaagctgt
     3061 agtgctgact tttgatgacg gctaccagag tttttatacc cgcgtcttcc caattcttca
     3121 ggccttccag tggcctgctg tatgggcccc cgtcggcagt tgggtcgata cgccagcgga
     3181 taaacaagta aaatttggcg atgagttggt cgatcgagaa tattttgcca cgtggcaaca
     3241 agtgcgagaa gttgcgcgtt cccggctcgt tgagctcgct tctcatacat ggaattctca
     3301 ctacggtatt caggctaatg ccaccggcag cttattgcct gtatatgtaa atcgtgcata
     3361 ttttactgac cacgcacggt atgaaaccgc agcagaatac cgggaaagaa ttcgtctgga
     3421 tgctgtaaaa atgacggaat acctgcgtac aaaggttgag gtaaatccac acgtttttat
     3481 ttggccttat ggcgaagcga atggcatagc gatagaggaa ttaaaaaaac tcggttatga
     3541 catgttcttc acccttgaat caggtttggc aaatgcgtcg caattggatt ccattccgcg
     3601 ggtattaatc gccaataatc cctcattaaa agagtttgcc cagcaaatta ttaccgtaca
     3661 ggaaaaatca ccacaacgga taatgcatat cgatcttgat tacgtttatg acgaaaacct
     3721 ccagcaaatg gatcgcaata ttgatgtgct aattcagcgg gtgaaagata tgcaaatatc
     3781 aaccgtgtat ttgcaggcat ttgctgatcc cgatggtgat gggctggtca aagaggtctg
     3841 gtttccaaat cgtttgctac caatgaaagc agatattttt agtcgggttg cctggcaatt
     3901 acgtacccgc tcaggtgtaa acatctatgc gtggatgccg gtattaagct gggatttaga
     3961 tcccacatta acgcgagtaa aatacttacc aacaggggag aaaaaagcac aaattcatcc
     4021 tgaacaatat caccgtctct ctcctttcga tgacagagtc agagcacaag ttggcatgtt
     4081 atatgaagat cttgccggac atgctgcttt tgatggcata ttgttccacg atgatgcttt
     4141 gctttcagat tatgaagatg ccagtgcacc ggctatcacg gcttatcagc aagcaggctt
     4201 tagcgggagt ctgagcgaaa ttcgacaaaa cccggagcaa tttaaacagt gggcccgctt
     4261 taaaagtcgt gcgttaactg acttcacttt agaacttagt gcgcgcgtaa aagccattcg
     4321 cggtccacat attaaaactg cacgaaatat ttttgcactt ccggtaatac aacctgaaag
     4381 tgaagcctgg tttgcacaga attatgctga tttcctaaaa agctatgact ggaccgctat
     4441 tatggctatg ccttatctgg aaggtgtcgc agaaaaatcg gctgaccaat ggttaataca
     4501 attgaccaat caaattaaaa acatccctca ggctaaagac aaatctattt tagaattaca
     4561 ggcacaaaac tggcagaaaa atggtcagca tcaggctatt tcttcgcaac aactcgctca
     4621 ctggatgagc ctattacaac tgaatggagt gaaaaactat ggttattatc ccgacaattt
     4681 tctgcataac caacctgaaa tagaccttat tcgtcctgag ttttcaacag cctggtatcc
     4741 gaaaaatgat taatcgcatc gtatcgtttt ttatattatg tctggtgtta tgcatacccc
     4801 tatgcgtagc gtactttcac tctggtgaac tgatgatgag gttcgttttc ttctggccgt
     4861 tttttatgtc cattatgtgg attgttggcg gcgtctattt ctgggtctat cgtgaacgcc
     4921 gctggccgtg gggagaaaac gcaccagctc cccagttgaa agataatccg tctatctcca
     4981 ttatcattcc ctgttttaat gaggagaaaa acgttgagga aaccatacac gccgctttag
     5041 cacagcgtta tgagaacatt gaagttattg ccgtaaatga cggttcaaca gataaaaccc
     5101 gtgccatcct ggatcgcatg gctgcacaaa ttccccattt gcgggtcatt catctggcgc
     5161 aaaaccaggg gaaagccatt gcgcttaaaa ccggagctgc cgcggcgaaa agtgaatatc
     5221 tggtgtgcat tgatggcgat gcgttattag accgcgatgc ggcggcatat attgtggaac
     5281 cgatgttgta caacccgcgt gtgggtgccg taaccggtaa tcctcgtatt cgaacacgtt
     5341 ctaccctggt gggtaaaatt caggttggcg agtattcctc aattattggt ttgatcaagc
     5401 gaacccagcg tatctatgga aacgtattta ccgtttccgg tgttattgcc gcatttcgtc
     5461 gcagcgccct ggcagaagtg ggttactgga gtgacgatat gatcaccgaa gatattgata
     5521 ttagctggaa gctgcagttg aatcagtgga cgatttttta cgagccacgg gcactgtgct
     5581 ggatattaat gcctgaaacg ttaaaagggc tgtggaaaca gcgcctgcgc tgggctcagg
     5641 gcggtgcaga agtattcctc aaaaatatga caaggttgtg gcgcaaagaa aactttcgaa
     5701 tgtggccgct gttttttgaa tacagcctga cgacaatatg ggccttcacc tgcctggtcg
     5761 gtttcattat ttacgcagtc caacttgccg gtgtaccgtt aaatattgaa ttgacacata
     5821 tcgctgcgac acatactgcc ggaatattat tgtgtacgtt atgtttactg caatttattg
     5881 tcagcctgat gatcgagaat cgctatgagc ataatctgac ttcatcgctt ttctggatta
     5941 tttggttccc ggttattttc tggatgctga gcctggcaac gacattggta tcatttacac
     6001 gagtcatgtt gatgcctaaa aagcaacgcg cccgttgggt aagtcccgat cgcgggattc
     6061 tgagaggtta atatgaacaa tttaattatt acgacccgac aatcaccagt acgtttactg
     6121 gttgattatg ttgccacaac catcttgtgg acattatttg cgttgttcat attcttattc
     6181 gccatggatc tgctgacggg ttattactgg caaagcgagg ccagaagccg acttcagttc
     6241 tattttttgc tggcagtggc gaatgccgtc gtgttaattg tctgggcgct gtacaataag
     6301 ctgcgttttc aaaaacagca gcatcatgca gcctaccaat atacgccgca agaatatgca
     6361 gagagcttag caatacctga tgagctctat cagcaactac aaaaaagcca caggatgagc
     6421 gtacacttca ccagccaggg gcaaataaaa atggttgttt cagaaaaagc gctagtcagg
     6481 gcataaacac ccaaaacaaa gcccggttcg cccgggctct gcaccgataa tacacttaac
     6541 tgtatgcatg cagcgtacgt tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc
     6601 ggacgatccc aaccatttca tcttcttcga aacgttccag cgcgtcactt aatccggagc
     6661 acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt gacgataacc gtcacgttct
     6721 ccccgaggcg ggttaaaaac attttcattt gcgcggcagt cacattctgc gcctcgtcaa
     6781 gaatgacgac tgcattttca aaggtacgtc cacgcatata ggcgaacggc gcaatttcca
     6841 ccttccctat ttccggtcgc aggcagtact gcataaagga agcccctaag cgccggacca
     6901 gcacgtcgta gaccgggcga aaatagggag caaacttttc tgcgatatct ccaggtaaga
     6961 agccaagatc ttcatcggct tgcagaactg gacgggtgac gataatcctg tcgacatcct
     7021 tatgtatcag ggcctctgcc gcttttgctg cgctgatcca ggtttttccg cacccggctt
     7081 cgcccgtggc gaatatcagc tgcttactct caatagcctt cagatagtgc aattgcgctt
     7141 catttcgcgc gaggatgggc gaagtatcgc gactgtcgcg ggccatacca atggcttcta
     7201 cgccgcccat ctgcacaagc gaggtgaccg attcttcttc acgctgctta tggctgcgcg
     7261 aatcccgtct cagcacacgt tttgcctcgc gacgagcttt gatcactgct ttttgtcttc
     7321 ccatggagag caccttgagt tgtttgtatt catcacacgc gccgttggca gcgcgattat
     7381 gcgcacgaac atcagagggt tggcttcctt gtaagccata gtttgctttt ggataaaatg
     7441 ccgaaaaacg gctacgcgca ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag
     7501 gttgaaaatg caaagtgacg agatgactac cggaggagaa aactccgcga gtggtggcgc
     7561 gttgattatc taaaacatgt ccagtacagg acgttaccat ccgcgatctc catagtgact
     7621 gactatcact gccgggaact tccgctgcta cttaataagt acaacagttc tcgcatttat
     7681 tgcaacaata tatttactta tatttaacta taaaacacca tttcagtgac attagtttct
     7741 actggaaaga tgacagagtg atgacagata tgaaaaaagc tgtgtgcttt cagcaggatt
     7801 tgcatcaggc atcagtgcac gatcgccgta tgcggcgtga acgccttatc cgcctaccga
     7861 acccgcaccg attgtaggca taagaagacg cgcaagcatc gcatcaggca tctgcgcaag
     7921 aacgccttat ccgtctaaaa acattaaacc cgcaataacg cgcttccgag ataatcgttc
     7981 gcgtccttca ctcccggcag cgcaaaaaaa taaccgccgc cgataggttt aacgtattcc
     8041 tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc cagatcgtgt
     8101 tggtaacaga caaacagtag ccccatatcc agttgcccgg agttggtgac gcccagtgaa
     8161 taactgtagc cacgacgcag catcaggctg gactcactct ccgccgtgcg gggattcgcc
     8221 agccggatat ggctgtccag cgcaatcccc ttcccttccg ggtcgctggc gtaatcaggc
     8281 acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg gccaaaaatc
     8341 gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt
     8401 actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc cgttacccac
     8461 accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc gtctttgaaa
     8521 cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc ggaaataaac
     8581 ccttcccgct tccagcgcac gctgagcaaa tccggcgtat gtttgatgat atcgcgcagt
     8641 gcatggatca ccgtgtcctg ggtgttggcg caaatttgta gcaacacatc accatgacat
     8701 aacgccgcat ccagcgagtc gttgggaaaa cgcgtcatct tctgcaactt tttcggcatc
     8761 tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg
     8821 agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg caggcgcgga
     8881 tttggcgttt ctggtgccgc tccgccctga gtcagaaaag caaaacgctg agtcaacaag
     8941 cgaaacaacc gttcaagatc ggctttatcg ctggcaagca catcaaacgc caccagcatc
     9001 attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa cggctgtttc
     9061 tcattgcgtg caaccggtga aagcgtaccc ggcgcacttt gcgttttttg tgcatgagcg
     9121 accggacaac ttcccgccag cgccagcgca ccaatgcctt tcagtaaacg tcggcgtgac
     9181 ggttcattca cgccgttttt atcttcatac tgcataatgc ttaatccaat cccagcacac
     9241 cgcgaagttg cgccagatct tccgccagcg cagtaatcgg tcctttcagt gcattccggt
     9301 cggcatcggt caatttgtcg taggtttcaa aaccgtcttt agtacggtat ttcgccagaa
     9361 tggtatcgac ctttttaaag ttggcatcga cttttgccaa cagttccggg ttagcttttt
     9421 gcagttgtgg acgcagcaga tcgacaattt tctgcgagcc ttcaacgtta gcctggaaat
     9481 cccacagatc ggtgtggctg tagcgatctt cttcaccgct gattttgctg gctgccactt
     9541 cctcaatcag tccggctgca ccgccgacca cttttgaagg tgggaaagcc agttcactga
     9601 tgcgtttttg caaatcgacc acatcggtat aaagctggtc agcgtactga tccatgcctt
     9661 tggtggtgtt gtcgccaaac aatgcttttt ccagacggtg gaaaccggtg aattttggat
     9721 cagcggcttt ttgctcgtaa tcatcttcac gggcgtcaat gctgccatcc agatcggaga
     9781 acagttcagc aatcggttca atgcgctcat agtgctggcg cgtcggcgca tacagtgctt
     9841 tcgctttttc gatatcgcct gctttaatcg cgtcggtaaa ggctttggtg tcggtcacca
     9901 gctgtgtggt ttccgccatg acatacgctt tatatgcagt aattgcacca ccaagactta
     9961 acagcgcatc actttgcgcc gcatccgccg ttgcctcacc tttgacgatc aacttccctt
    10021 tcgggttagt cagcagaccg caggtcatat cgtattcgcc aggctgtaaa ttcgccgtca
    10081 ttttctggct aaagccaggg gcgatatttt cccgctcttc caccaccatc acgcctttga
    10141 ggatctccca ttccagcgcc ttctggctgt ggttctgaat aatgaactgt gttttcccgg
    10201 cgttaaccgt aatggtcatc ggttcgcact gcttatccgt cacggtcact ttgacctgcg
    10261 gcacatcagc ggcgttagcc ataaaagcag aagaaaacag cgcagccacg ctcaactgca
    10321 atgcgttacg gcggaagtta atggtcatga cgagactatc cctttaaagt atgttgtaac
    10381 taagcaagag tttgcgtcgt atttgttacg cggagcgaga cgctgtcgcc cctgcgcgtg
    10441 gtggcagagc aaatgccacc agcgccggga tgagataaat aaaccagacg gcgacttcgc
    10501 tgacgctcgg cgcttcctga tagccaaaaa tcccttccat cagcgtgcca aacagcgagt
    10561 gagttgagag caccgcactc atatcgaagg cgatttcctg aaagtggttc cacaatccgg
    10621 cttcatgaaa tgcgcgaatg gcaccagctg ccagccctgc ggcgacgaag agaataaaca
    10681 ggctggtcca tttaaaaaat gcaccaagat tgaggcgaat accgccccag tagagcagga
    10741 agcctagcac cacggcagta gcaagaccga gcattgcacc cagcggcggc cagatcccga
    10801 catcttgttg aaatgccgcc agcaggaaaa agaccgactc cagcccttcc cttgcaacgg
    10861 caaaaaagac catcatcacc agcgcccagc catgatgatt tccacgctgc aatgcgctat
    10921 cgacagcctg ctccagttgt actttgacgt tgcgcgacac tttgcgcatc cagaaaacca
    10981 tccaggtaag gatcaccacg gcgatcaccg ccacgatacc ttcaaacagt tcctgctctt
    11041 tttgcggaaa ttcgccggtg gtttcgttaa tgaagatccc caagcccagg cacaacgcag
    11101 cggcaagcaa cacgccaatc cacatcacac caatccatcg gcctcgctgg gtacgcttaa
    11161 gatagctggc aatcaaactg acaatcagcg cggcttcaag tccttcgcgc aacataatga
    11221 gaaacggaac aaacatgcca cccaccctta aacgtttttc tgagtcaatc tatgcaaaga
    11281 aaagtaaatc gcagtgatag cgattatcat tacgctaaag aaaaatacaa gcggaatatt
    11341 tataaaatga tgttggaatg taaaaatgag gagatcaaat ccggagaaaa aagcgattat
    11401 tttaaggtat ttagctgacg tttcgcccgg cagatgaagc ttgtacgaat gttggtggcg
    11461 acaaactcgc tgcagcgtgc ccgaagggtt gaattttcag ttagtcgttt cacaaaagaa
    11521 cgt
//
LOCUS       DAZFTN010000139        11457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFTN010000139 DAZFTN010000000
VERSION     DAZFTN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            364..1752
                     /gene="narU"
                     /locus_tag="ACHKV2_004261"
     CDS             364..1752
                     /gene="narU"
                     /locus_tag="ACHKV2_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO6942030.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1834..5574
                     /gene="narZ"
                     /locus_tag="ACHKV2_004262"
     CDS             1834..5574
                     /gene="narZ"
                     /locus_tag="ACHKV2_004262"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO6942031.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5571..7115
                     /gene="narH"
                     /locus_tag="ACHKV2_004263"
     CDS             5571..7115
                     /gene="narH"
                     /locus_tag="ACHKV2_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6942032.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7115..7810
                     /gene="narW"
                     /locus_tag="ACHKV2_004264"
     CDS             7115..7810
                     /gene="narW"
                     /locus_tag="ACHKV2_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6942033.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7807..8487
                     /gene="narI"
                     /locus_tag="ACHKV2_004265"
     CDS             7807..8487
                     /gene="narI"
                     /locus_tag="ACHKV2_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6942034.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8566..9459
                     /gene="yddE"
                     /locus_tag="ACHKV2_004266"
     CDS             8566..9459
                     /gene="yddE"
                     /locus_tag="ACHKV2_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO6942035.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9555..10399)
                     /gene="nhoA"
                     /locus_tag="ACHKV2_004267"
                     /pseudo
     CDS             complement(9555..10399)
                     /gene="nhoA"
                     /locus_tag="ACHKV2_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10698..11141
                     /locus_tag="ACHKV2_004268"
     CDS             10698..11141
                     /locus_tag="ACHKV2_004268"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO6942036.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11145..11372)
                     /gene="pptA"
                     /locus_tag="ACHKV2_004269"
     CDS             complement(11145..11372)
                     /gene="pptA"
                     /locus_tag="ACHKV2_004269"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO6942037.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2699 a         2974 c         3014 g         2770 t
ORIGIN      
        1 accgatagac tttctggaca gatcgttttc ctccagctca tggccttttt atcactaatt
       61 ctataagtgt ctggtcttgt taaaaatata gttaatatac aaccctttgg attcatcatc
      121 tgaagggttg aatcatgaca tgcgtacata gcttaacccc gtttaatggt tagcttgtta
      181 gttacattta gtaacacata actggctgct taatccccat tctgcccccg cggttacgct
      241 ttcttatcta ttctttcaat cagcacttca cccatttcat ccttaattct aataactggc
      301 tattgcgcct tacctcctgt tttgtctttt tttcattacc aatgtgtaca tgtgaggaac
      361 aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg gaaaccagaa
      421 aatccggcct tctggaaaaa taaaggaaaa catattgctc gaagaaacct ctggatatca
      481 gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt taccgttaat
      541 ctcaataaaa tcggttttaa tttcactacc gatcaactct ttttattaac cgcattaccc
      601 tccgtttctg gcgcattatt acgtgtcccc tactccttta tggtgcctat attcggtgga
      661 cgtcgatgga cggtttttag tactgcaatc ctgattattc cttgcgtctg gctcggaatt
      721 gccgtgcaga atccgaatac tccttttggg atatttatcg ttatcgcttt gctatgcggt
      781 tttgcaggtg cgaactttgc ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc
      841 aggcaaggta gcgctcttgg gattaatggc ggattaggaa acttaggtgt aagtgtaatg
      901 cagctggttg caccgctggt catttttgta cccgtatttg cctttctcgg cgtcaatgga
      961 gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat ttgggtaccg
     1021 ctactagcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc cagttcacgc
     1081 gcgtcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct gctgagcctg
     1141 ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggtttcgc catgctggca
     1201 aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc atttatcggt
     1261 gccatcgcgc ggtcggtggg tggtgctatt tccgataaat tcggcggcgt gcgggtgacg
     1321 ttgatcaact ttatttttat ggcgattttc agcgccctgc tgttccttac cttaccgggc
     1381 acaggctccg gtaacttcat cgccttttac gccgtattta tggggctgtt tctgaccgcg
     1441 ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca gataaccatt
     1501 tatcggatga agatgaaagg cggtagtgat gagcaagctc agagagaagc cgtcaccgaa
     1561 acggcggcgg ctctgggctt tatctcagcc atcggcgcag tgggcggctt ttttattccg
     1621 caggcatttg gcatgtcact caatatgacc ggctctccag tgggcgcgat gaaagtgttt
     1681 ttaatcttct acatcgtttg tgtgctactg acctggctgg tttatggtcg gcggaagttc
     1741 agccaaaaat aagagatcga tgcctgatgt acaaccgtgt cgcatccgac aaggcatcag
     1801 atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga tcgctttcgc
     1861 tacttcaaac aaaagggcga tacctttgcc gatggtcacg gacaggtgat gcatagcaac
     1921 cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt gcgttccacc
     1981 cacggtgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa tggtctggtg
     2041 acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc caatcatgaa
     2101 cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc taaccgcctg
     2161 aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct caaacaacac
     2221 agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagag cctgagctac
     2281 aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggccggaact aaaccagctg
     2341 attgccgccg ctaacgtctg gactatcaaa acctacggcc cggatcgcgt tgccggtttc
     2401 tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacacgtta tctgtcacta
     2461 ttaggtggca cctgtttaag cttctacgac tggtattgcg acctgccgcc cgcctcgccg
     2521 atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa ctccagttac
     2581 atcatcgcgt gggggtctaa cgtaccgcag acacgtacgc cggacgccca tttctttacc
     2641 gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc tgaagtggcc
     2701 aaattgtgcg accagtggct ggcaccaaaa caaggcactg atagcgccct ggcgatggca
     2761 atgggccatg tgattttaaa agagtttcat ctcgataatc ccagcgacta ctttatcaac
     2821 tactgccgcc gctatagcga catgccgatg ctggtaatgc tggaacctcg cgacgatggt
     2881 agctacgtac ccgggcgcat gatccgcgcg tctgacctgg tagatggact aggcgaaagc
     2941 aataatccgc agtggaaaac cgtggcggtt aataccgcag gtgaattggt agtgccgaat
     3001 ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc cattgccgcc
     3061 ggtaaggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgccgt tgcaggcgtg
     3121 gctttcccct actttggcgg catcgaaaat ccgcattttc gcagcgtcaa acacaatcct
     3181 gtcctggtgc gtcaattacc cgttaaaaac ctgacattag ccggtggcag cacctgtcca
     3241 gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg gctggaagat
     3301 gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg gggtgagcaa
     3361 attaccggcg tgccgcgcca gtatattgaa accatcgctc gtgaatttgc cgatactgcc
     3421 cataaaacgc atgggcgctc gatgattatc ctcggcgcag gtgttaacca ctggtatcac
     3481 atggacatga actaccgggg aatgatcaat atgctgatct tctgcggttg tgtcggacaa
     3541 agcggtggcg gctgggcaca ctatgtcggt caggaaaaac tgcgcccgca aaccggctgg
     3601 ttgccgctgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa cagcacctcg
     3661 tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgcgca ggagttactt
     3721 tcaccgcttg ccgatgccag taagtacagc ggtcatctga ttgattttaa cgttcgcgcc
     3781 gaacgcatgg gctggctacc ttctgcgccg cagttggggc gtaacccgct cgggattaaa
     3841 gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc gctgaaatcg
     3901 ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc gcgtaatttg
     3961 tttgtctggc gttctaacct gcttggctcc tccggcaaag gccacgagta tatgcagaag
     4021 tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag cgacgggatc
     4081 aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga cctgctggtg
     4141 acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct gcccaccgcc
     4201 acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat tcatccgctt
     4261 tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta caaaggtatt
     4321 gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga cgtggtatta
     4381 caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt gctcgactgg
     4441 cgcaaaggcg aatgcgatct gagcccgggt aaaaccgcgc cgaatattgt ggcggtggag
     4501 cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat ggacaaactt
     4561 ggcaacggcg gtaaagggat ttcgtggaat acgcaggatg aaatcgattt cctcggtaaa
     4621 ctcaattaca ccaagcgtga tggcccagcg caggggcgtc cgctgattga caccgccatt
     4681 gacgcttcag aagtgattct ggcactggca ccagaaacca acggtcatgt tgcagttaaa
     4741 gcgtggcagg cgctgggcga gatcaccgga cgcgaacata cccatctggc gctgcacaaa
     4801 gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat tatctccagc
     4861 cccacatggt ctggtctgga aagcgatcac gtctcctata atgcgggata caccaacgtt
     4921 catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca ggatcatccg
     4981 tggatgcgtg cttttggtga aagcctggtg gcatatcgcc cgcctatcga cacccgtagc
     5041 gtcagtgaga tgcgccagat cccgccaaac ggcttcccgg aaaaagcact taacttcctg
     5101 acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct aatgctgacg
     5161 ctctctcgcg gtggaccgat tgtctggatc agcgaaaccg atgcccgtga actaaccatt
     5221 gtcgataacg actgggtgga agtgtttaac gccaatggcg cgctgacggc ccgcgcggtg
     5281 gtcagccaac gtgtaccgcc gggtatgacc atgatgtatc acgctcagga acgcattatg
     5341 aatattcctg gttcggaagt aactggcatg cgcggcggta ttcataactc ggtcacccgc
     5401 atttgcccga aaccaacgca tatgattggc ggttacgcgc agctggcctg gggctttaac
     5461 tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa gatgaagaac
     5521 gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa atgaaaatcc
     5581 gttcgcaagt cggcatggtg cttaatctcg ataaatgtat cggctgccat acctgttcgg
     5641 tgacctgtaa aaacgtctgg accggacgcg aaggcatgga gtacgcatgg tttaacaacg
     5701 tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag
     5761 gcggctgggt ccgcgatgtg aatggcaaga tacgcccgcg tctgggcagc aagatgggtg
     5821 taataaccaa aatcttcgcc aacccggtgg tgccgcagat tgatgattac tacgaacctt
     5881 tcaccttcga ctacgaacat ttgcatagcg caccggaagg caaacatatc cctactgctc
     5941 gcccgcgttc gctgattgac ggtaagcgga tggacaaagt gatctggggg ccaaactggg
     6001 aagaactgct gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc gaggccatgc
     6061 aaaaggagat gtacgggcag tttgaaaaca ccttcatgat gtacctgccg cgcctgtgcg
     6121 aacactgcct caatcccagc tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg
     6181 aagaagacgg cattgtgctg attgatcagg ataaatgccg tggctggcgt ttgtgcatta
     6241 gcggttgtcc gtacaaaaaa atctacttca actggaaaag cggcaagtca gaaaaatgta
     6301 tcttctgtta cccacgaatt gagtccggtc aaccgaccgt gtgctcagaa acctgcgtgg
     6361 gtcgcatccg atatctgggc gtgctgcttt acgacgctga ccgcatcgag gaagcggcga
     6421 gcaccgagcg cgaggttgac ctctatgaac gccagtgcga agtgttcctc gatccgcacg
     6481 atccctcagt gatcgaggaa gccctgaaac aaggtattcc acaaaatgtg attgacgctg
     6541 cccagcgttc gccagtctac aaaatggcga tggactggaa actggcacta ccgctgcacc
     6601 ctgaataccg caccctgccg atggtctggt acgttcctcc gctgtcaccg attcagtcct
     6661 acgcagatgc gggcggtttg ccgaaaagcg aaggcgtgct gcccgccatc gaaagcctgc
     6721 gtattccggt gcaatatctc gccaatatgt tgagtgccgg cgataccggt ccggtactgc
     6781 gggcgctgaa acggatgatg gcgatgcgcc actatatgcg ttcacaaacc gtggaaggcg
     6841 ttactgatac tcgtgccatc gacgaagtag gcctgagcat cgcccaggtc gaagaaatgt
     6901 atcgctatct cgccattgcc aactatgaag atcgttttgt tatcccgacc agccatcggg
     6961 aaatggcggg cgatgccttc gcagaacgca atggctgcgg ttttaccttt ggtgacggtt
     7021 gtcacggctc ggacagcaaa ttcaacctgt tcaacagtag ccgtatcgat gccatcaaca
     7081 tcaccgaagt gcgcgacaaa gcggagggcg aataatgcag atcctcaaag tgatcggcct
     7141 gttgatggag tatccggacg agatgttgtg ggaatgcaag gaggacgcgc tggcgttgat
     7201 ccgccgcgat gcgccgatgc ttacggattt cacccgcaac ctgcttaacg cgccgctgct
     7261 ggataaacag gccgaatggt gcgaagtgtt tgaccgcggg cgcaccacgt cgctgctgct
     7321 gttcgaacat gttcatgccg agtcccgcga tcgcggccag gcaatggtgg acctgctggc
     7381 ggagtatgaa aaggtcggcc tgcagctgga ttgtcgggaa ctgcccgatt atctaccgct
     7441 gtatctggag tatttaagcg tactgcctga cgatcaggcg aaagaaggat tgctcaacgt
     7501 tgcgccgatc ctcgccctgc ttggcggtcg cttaaaacaa cgcgaggcac cgtggtacgc
     7561 gttgtttgat gctctgctgc aactggcggg aagtacactt tcaagtgaca gtgtcacaaa
     7621 acaagtaaac agcgaagagc gcgatgacac ccgccaggcg cttgatgcag tgtgggaaga
     7681 ggaacaggtg aagtttattg aagataacgc cacggcgtgt gacagctcgc cgcttaatca
     7741 atatcagcga cgctttagtc aggatgtcgc gccgcaatat gtcgacatca gtgcgggagg
     7801 tgggaaatga ttcagtatct gaacgtcttt ttttacgata tctacccgta catttgtgcg
     7861 acggtgtttt tcctcggcag ctggctgcgc tacgactacg ggcagtacac ctggcgcgcc
     7921 tcctcaagtc agatgctcga taaacgcggg atggtgatat ggtcgaatct gttccatatc
     7981 ggcattttgg ggatcttctt cgggcacctg ttcggcatgt taacgccaca ctggatgtac
     8041 gcgtggtttt tgccagtggc agcgaaacaa ctaatggcga tggtgctcgg tggtatttgc
     8101 ggtgtgttga cgttgattgg cggcgcagga ctgctgtggc gcaggctgac aaatcagcgc
     8161 gtgcgtgcca cttccactac gccggatatc atcatcatga gcattttgct gatccagtgt
     8221 ctgttggggc taagtaccat accgttctca gcacagtatc ctgatggtag cgaaatgatg
     8281 aagctggtgg gttgggcgca aagcattgtc actttccgtg gtggatcgtc agagatgctt
     8341 aacggtgtag cgttcgtctt ccgcctgcat ctggtgttgg gaatgaccat cttcttgctc
     8401 ttcccattca cccgactggt gcatgtgtgg agcgccccgt ttgagtactt tactcgtcgg
     8461 tatcagattg tgcgctcgcg gcgataattt caccctggcc ggatagccct tcgctctccg
     8521 gccacttgca ggctatgata gttctccagt cacaggagaa cgttaatgaa accgcaggtt
     8581 taccacgtcg atgcttttac ctctcaacca tttcgcggca attctgccgg tgttgttttc
     8641 cccgccgata atcttagcga agcgcaaatg cagcttatcg cccgcgaatt aggtcattcg
     8701 gaaaccgctt ttctgctgca cagcgacgac agcgatgtgc gcatccgcta ctttacgcca
     8761 acagttgaag tgcctatttg cggtcacgcg acagtagctg cgcactatgt acgtgcgaag
     8821 gtgttaggtt taggaaattg cacggtctgg caaacatcgc tggcaggaaa acatcgcgtg
     8881 actatcgaaa agcaccacga tggttatcgt atttcgctgg aacaaggtac gccaggcttt
     8941 gagccaccgc tggaaggtga aacacgtgcg gcgattatca acgcgcttca tctcactgag
     9001 gacgatattc tgccaggctt gccaattcag gtggcaacca caggacactc aaaagtgatg
     9061 atcccactga aaccggaagt ggatatcgac gccctttcgc ctgaccttaa tgcgctgacc
     9121 gctatcagca aacagattgg ctgcaatggt ttcttcccgt tccagatccg cccaggcaaa
     9181 aacgaaaccg atggtcgcat gttctcgcct gcgattggta ttgtggaaga tccggtgact
     9241 ggcaatgcca atggcccgat gggtgcatgg ttggtacatc acaacgtatt gccccacgat
     9301 ggcaacgtgt tgcgtgttaa aggccatcag gggcgcgcat tggggcgtga cggtatgatt
     9361 gaagtgacgg tgacaattcg tgataaccaa ccggaaaaag tcaccatttc tggcgcggcg
     9421 gtgattttgt ttcatgccga atgggcaatt aaactctgaa accgttgcct gatgcgcttc
     9481 gcctgtcagg tctacaaccg ggctaatgta tgccggataa ggcgtttacg ccgcatccgg
     9541 caacctgaca taaattattt tcccgcctcc gggtgcgtat caaacgccgc catcaccagc
     9601 gctaactcat ccacggtaaa gccatgtttc gcatcatcca cgcccagacc aaactgttct
     9661 tgcatcacag catataacga cgccacatcc ggtagatttc gctgctccac cgcgtgccca
     9721 ttttcataat gggaaaatga aaattggtca gtgtcagctt gccgccgtcc ggcaaatggc
     9781 ggcacatcag caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac cagaaattac
     9841 ccatcacata atcgctttgt tgctgctcgc agagatcaaa acggtacatc gactgccagt
     9901 gctgatgatg attaaactgc aacacccaat catcaccctc ctgcaacaac cgatactctc
     9961 cgtgtggcgt ggtctgcaca agatcgggaa ctaaacgaat cggcgcggtt agcgtctgcc
    10021 caccgaaacc gacatcagca atccattttt cctcttccag ttccaccaac agcaaacgat
    10081 gggtgcgcgg cggtaatgct ggcggatttg ataacactac ccgccctaac aagctgcgaa
    10141 cgttaaaccc cagctcgcgt aacacccgct caaaaacgcc attctgctca aaacagtaac
    10201 cgccacgacg ggctatcacc agtttctctt ccagcgattg atcatcaagc tgcatttccc
    10261 tcggcagcaa aacgtcgagg ttttcaaacg gaatggtgca attgtgtttc aggtgcaatg
    10321 cacgaagcgt atcaatattg accgcagcag ctcccgacca gttaatacgg gcaaaatagt
    10381 gattcagaat gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt ataacctgga
    10441 atagacgccg caccggctat tttgtgcgat cgcggacatg catcacgttc gcaaatcgcc
    10501 aatcccgtta atttcgttac ttattgcgtt acggactacg cttaaaaaaa ccttcttcgc
    10561 gaggcgatac catgagccga ttcatcccca tagaattaca ccatgccagc cggctgttaa
    10621 atcatggccc aactgtcatg atcaccagtt tcgatgagca atcccagcgg cgtaacatta
    10681 tggctgcagc ctgatcaatg ccggtggagt ttgaacctcc acgcgtgacg attgtggtag
    10741 ataaatcgac atggaccagg gagttgattg aacgtaacgg taaatttggc atcgttatcc
    10801 cgggcgttgc agcaactaac tggacgtggg cagtgggaag tgtgtcgggt cgtgaagaag
    10861 ataaatttaa ttgctatggc attccggttg tgagaggccc ggtatttggt ttgcctctgg
    10921 tcgaggaaaa atgtctggcg tggatggagt gtcgattgct acctgcgact tctgcgcaag
    10981 aagaatacga cacgctgttt ggcgaagtag tatcagcagc ggcagacgca cgggtatttg
    11041 tcgaaggccg ctggcagttt gatgatgata agctcaatac gttgcatcat ttaggtgctg
    11101 ggacgtttgt taccagcggc aagcgtgtta cggcgggtta agcattacat gctgtaaccg
    11161 ggtttcttta tcaaagcctc catttggggc gcgatttcgg catcccagat agcttgccaa
    11221 gattctggtt gaatctgttg tagagcaatg cttatcgaac tgtctttact gttcagatgg
    11281 cgaataataa cgtcggtaat atctgcagca agtgctgctt tttgttgttc gtccagttca
    11341 cgcggaaaac atttaatgtc tatgtgcggc atgattacgc tccttttgaa agatgtcagt
    11401 aaaattaaca cacattgtga atgtcgtgtg tccggtaaag cgtttttacg acaataa
//
LOCUS       DAZFTN010000140        11254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFTN010000140 DAZFTN010000000
VERSION     DAZFTN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..134
                     /locus_tag="ACHKV2_004270"
     CDS             3..134
                     /locus_tag="ACHKV2_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6942038.1"
                     /translation="MYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            113..217
                     /gene="speFL"
                     /locus_tag="ACHKV2_004271"
     CDS             113..217
                     /gene="speFL"
                     /locus_tag="ACHKV2_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO6942039.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            528..2726
                     /gene="speF"
                     /locus_tag="ACHKV2_004272"
     CDS             528..2726
                     /gene="speF"
                     /locus_tag="ACHKV2_004272"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO6942040.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2723..4042
                     /gene="potE"
                     /locus_tag="ACHKV2_004273"
     CDS             2723..4042
                     /gene="potE"
                     /locus_tag="ACHKV2_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO6942041.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4099..5739)
                     /gene="pgm"
                     /locus_tag="ACHKV2_004274"
     CDS             complement(4099..5739)
                     /gene="pgm"
                     /locus_tag="ACHKV2_004274"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO6942042.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5765..6310)
                     /gene="seqA"
                     /locus_tag="ACHKV2_004275"
     CDS             complement(5765..6310)
                     /gene="seqA"
                     /locus_tag="ACHKV2_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO6942043.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6495..7259
                     /gene="ybfF"
                     /locus_tag="ACHKV2_004276"
     CDS             6495..7259
                     /gene="ybfF"
                     /locus_tag="ACHKV2_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6942044.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7399..7692
                     /gene="ybfE"
                     /locus_tag="ACHKV2_004277"
     CDS             7399..7692
                     /gene="ybfE"
                     /locus_tag="ACHKV2_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO6942045.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7832..8362
                     /gene="fldA"
                     /locus_tag="ACHKV2_004278"
     CDS             7832..8362
                     /gene="fldA"
                     /locus_tag="ACHKV2_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO6942046.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8572..8658
                     /gene="uof"
                     /locus_tag="ACHKV2_004279"
     CDS             8572..8658
                     /gene="uof"
                     /locus_tag="ACHKV2_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO6942047.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8651..9097
                     /gene="fur"
                     /locus_tag="ACHKV2_004280"
     CDS             8651..9097
                     /gene="fur"
                     /locus_tag="ACHKV2_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO6942048.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9181..9507)
                     /gene="chiQ"
                     /locus_tag="ACHKV2_004281"
     CDS             complement(9181..9507)
                     /gene="chiQ"
                     /locus_tag="ACHKV2_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO6942049.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9557..10962)
                     /gene="chiP"
                     /locus_tag="ACHKV2_004282"
                     /pseudo
     CDS             complement(9557..10962)
                     /gene="chiP"
                     /locus_tag="ACHKV2_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2739 a         2775 c         2801 g         2939 t
ORIGIN      
        1 tgatttatta tctggggtat gatcgttttt tgttgatctt cttcacagat tatagccatt
       61 tcatggatag aataactcta ccttcaactg acacagcaag aggtaaaggt aaatggaaaa
      121 taataaccgc ttaatgcctc atataaggcg gacaacccat atcatgaagt ttgcccatcg
      181 taatagtttt gactttcatt tctttaatgc ccgctagtct tctgactaaa gggcacccca
      241 gcgtacaggt ctccctgact ttaagcatta caggttaata cctgtatccc tcggtgctca
      301 tatactgcta acccttttaa actctacata attcgagtcg cagcatatgc aacttgaagt
      361 atgacgagta tagccagtta ccgggctggt ctgggttatt gcatctgcaa aaagcaaact
      421 actgatttat ttatcagcgg tggagctttg ctttttttcc ggcgtgatcg atttctcctt
      481 tgagaaattg aggacctgct attacctgaa ataaagagat gaacaaaatg tcagaattaa
      541 aaattgccgt tagtcgttct tgcccggatt gtttttccac tcatcgtgca tgcgtgaata
      601 tagacgaaag taattatatt gacgttgccg ccattatttt atcagtcagt gatgttgaac
      661 gtggaaaact cgatgaaata gacgctactg gctatgacat tcctgttttt attgcaacgg
      721 aaaatgaaga acgtatacca gcagaatatc tttcacgtat ttctggtgtc ttcgaacatg
      781 gagaagcacg caaagaattt tatggtcgtc agttagaaac cgctgccagc cattatgaaa
      841 ctcaactgcg cccacctttc tttcgcgcac tggtcgatta tgtcaatcag ggcaacagcg
      901 catttgactg ccccggtcat cagggcggcg aatttttccg tcgccatccg gcggggaatc
      961 agtttgtgga atactttggt gaggcgctgt tccgtgccga cttgtgcaac gccgacgtag
     1021 cgatgggcga tctgctgatt cacgaaggcg cgccatgcat tgcacagcaa catgcagcaa
     1081 aagtgtttaa tgccgataaa acctacttcg ttttaaatgg cacttcatct tctaacaaag
     1141 tggttttaaa cgccctgctg acaccgggtg atctggtgct atttgaccgc aataaccata
     1201 agtctaacca ccacggggcc ttgctacagg ctggtgcaac accggtttat ctggaaacag
     1261 cacgtaaccc gtatggtttt atcggtggta ttgatgcaca ctgcttcgaa gaaagttatc
     1321 tgcgtgaact gattacagaa gttgcaccgc aacgggcaaa agaggcgcgt ccgttccgcc
     1381 tcgctgtgat tcagctaggc acctacgacg gtacgattta taacgcccgc caggtggtgg
     1441 ataaaattgg tcatctgtgt gactacatcc tgtttgattc cgcatgggtc ggctatgaac
     1501 agtttattcc gatgatggct gactgttcgc cgctgttgct ggatcttaat gagaacgatc
     1561 cgggtattct ggttacgcaa tccgtacata aacagcaggc tggtttttct caaacttcac
     1621 aaatccataa aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca
     1681 tgaacaacgc ctttatgatg catgcctcca ccagcccgtt ctatccgctg tttgccgcac
     1741 tggatatcaa cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg atggattgtg
     1801 tggtaaatgg cattaatgcc cgcaaactga tcctcgataa ctgtcagcat attcgtccgt
     1861 tcatacctga actggtggat ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg
     1921 ttgatctgcg cttcttccag tttgtaccag gtgagcactg gcattctttt gaaggctatg
     1981 cagagaatca atactttgtt gatccgtgca aactgttgct gacaacccca ggtattgatg
     2041 cacgtaacgg cgaatatgaa gcgttcggtg tacccgcgac gattcttgct aacttcctgc
     2101 gcgaaaatgg cgtagtgccg gaaaaatgcg atcttaactc catcctcttc ctgctgactc
     2161 cggcagaaga tatggccaaa cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac
     2221 tgctggaatc tgatgcgccg ctggcggaag tgctaccttc catctacaaa cagcatgaag
     2281 agcgctacgc cggttatacc cttcgtcagt tgtgccagga aatgcatgat ttgtatgccc
     2341 gccacaacgt gaaacaactg caaaaagaga tgttccgtaa ggaacacttc ccacgcgtca
     2401 gcatgaatcc gcaagaagct aactacgcct atttacgcgg tgaagtggag ctggttcgtc
     2461 tgccggatgc agaaggccgt atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc
     2521 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
     2581 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
     2641 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
     2701 ccctgctgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
     2761 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
     2821 gcttgccgaa gttgggacaa tctcaattat ctcctggctg gtgacagccg taggctcaat
     2881 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg
     2941 cggttatgcc gagtatgctt tcggtaaatc cggtaacttt atggcgaact atacctacgg
     3001 cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
     3061 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
     3121 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
     3181 tgtgtgggga gtcattattc cggtcgtcgg tctgtgcatt attggctggt tctggtttag
     3241 cccgacgctg tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcggtagg
     3301 ttcttccatc gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt gtgcaaatac
     3361 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
     3421 tgcggcggtg atttatatcg tctccaccaa cgtgattgcc gggattgtgc caaatatgga
     3481 gctggcaaat tcaacggcac catttggtct ggccttcgcg cagatgttca cgccggaagt
     3541 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
     3601 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
     3661 ctcccatgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
     3721 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
     3781 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
     3841 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
     3901 tgttggcgcg atgtacagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
     3961 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
     4021 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgccct
     4081 ttttcttatt taatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc
     4141 tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga tcttatatgc
     4201 gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc
     4261 gccaatagaa gcaccgttgc ccggagcagc agtcaggcgc gcggtgatcg ggtcaccagc
     4321 cagggtgctg gcactcacca tttccggaga cagcttagac agcgccgctt tttgtgcgga
     4381 agtcgcagct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt
     4441 gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca gcagacacat
     4501 gatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc
     4561 tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa caaaccattt
     4621 gaaacctacc gggacttcta ccagtttacg gcccaagtcg ttgaccacac ggtcgatcat
     4681 cgcagatgaa accagcgttt taccgacggc aacagctttg ccccactgcg gacgatgctg
     4741 gaacaggtag ttgattgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt
     4801 gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg
     4861 cagtgccagc aggcccgcca tcgcacactc ggaggagcag tccatacgga tcgcgccgtc
     4921 tttatcaagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga tggtcaggtt
     4981 gaggttgtaa tactcgccaa tacgcttcca gtattcgata ccggaaccgc ccagcggatc
     5041 aacgccaagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat cggccagccc
     5101 ttccacaaac ggctgcacca gatcctgctc tttcacatga ccggatgcca tcgcttcgtc
     5161 gagggagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg ctctgtcttc
     5221 caccacttta gtgacgttgg tatcagccgg gccaccattt ggcggattgt acttgatacc
     5281 accatcttcc ggcgggttat gggacggtgt aataacgata ccgtctgcca gcgggccacc
     5341 ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggggtga agccattgtt
     5401 ttcctgcaca ataacatcta cgccgttcgc tgccagcact tccagaacgg aaataaatgc
     5461 aggttcggac agggcgtgag tatctttacc cacatagcaa gggccagtga tgccgttttt
     5521 cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg
     5581 gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct ccgcattccc
     5641 tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact
     5701 ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa
     5761 cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc atcgactgca
     5821 tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg
     5881 gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat
     5941 ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct
     6001 gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt
     6061 gctctgcata ttcatccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg
     6121 ttttgatcgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg
     6181 gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc
     6241 tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa
     6301 tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca ggagtatatg
     6361 aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct
     6421 tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa
     6481 aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata
     6541 attctcccat cgttcttgtc catggtctgt ttggcagcct cgacaacctt ggcgtactgg
     6601 ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt
     6661 caccgagaga tccggtaatg aattacccgg cgatggcgca ggatcttgtt gatactctgg
     6721 atgcacagca gatcgacaaa gcaacattta tcgctcactc catgggcggt aaagcggtaa
     6781 tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc gatatcgcac
     6841 cggtcgacta tcacgtacgc cgtcatgatg agattttctc ggctatcaac gcggtcagtg
     6901 aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat cttaatgaag
     6961 aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc
     7021 cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc
     7081 accctgccct gtttatccct ggtggtaatt ctccgtatgt tagcgagcag taccgtgatg
     7141 atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg
     7201 tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac
     7261 gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc
     7321 atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc
     7381 cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc
     7441 gcgcacgagc gtcgaccggg acgaccgaaa acaaatccgc tttcgcgcga tgaacagctg
     7501 cgtattaata aacgcaacca actaaaacgc gacaaagtac gtggcctaaa gcgtgtcgaa
     7561 ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc gcgcaatatg
     7621 agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag
     7681 ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat
     7741 tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca
     7801 tttcaataag tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag
     7861 cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt
     7921 tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt atgacattct
     7981 gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg atgacttctt
     8041 cccgactctc gaagagattg atttcaatgg caaactggtt gcgctgtttg gttgtggtga
     8101 ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg acatcattga
     8161 accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt tcgaagcatc
     8221 aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc
     8281 ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct
     8341 cgacgaaatt ctcaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat
     8401 agataatgcc aatcaaaata attgctacaa atttgtaact tttgccgttg tacctgtaca
     8461 atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta
     8521 acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc
     8581 attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt agtaacagga
     8641 cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct
     8701 tcctcgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga
     8761 agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg
     8821 cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg aaggcggtaa
     8881 atccgtattt gaactgacac agcaacatca ccacgatcac ctgatctgcc tcgactgcgg
     8941 caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa
     9001 acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgcg ccgaaggcga
     9061 ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agtctgaacg agaaaagcca
     9121 acctgcgggt tggctttttt atgcaaggga aataagaaaa gcgagagtta cagctctcac
     9181 ttatttgtta ttactgcgaa tttcctgcca gaccttatca caatcggctt tcaccgcctg
     9241 atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc ggacactttc
     9301 ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca cgctctggca
     9361 ggcttcaatt ttttccggcg aaccttgtgc ggtgttgata caggcgctgt atgcctcttt
     9421 cagacggctg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc ccgatgccat
     9481 catggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc aggcacaaaa
     9541 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat tttacgtcac
     9601 gctcatcctg gaagatgttg ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
     9661 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
     9721 agaccgcatc caggctgtat gcagactctt caatagtccg gtttttgtcg tagtacgcat
     9781 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
     9841 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
     9901 tttcgccgtt ggcgttgaag tcagaacggt tatcccacca gatatccagg cgaccgttcg
     9961 aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg tcagctttaa
    10021 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
    10081 cttgcagcca ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
    10141 gcgtaccgta gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
    10201 tggctttggc aaagtattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
    10261 cgaagttatt tttgaagtcg tatttcgccc caagggagtg cagataatca actttggtgg
    10321 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggtgctttg tattcgttgg
    10381 tccacatgta ggagaaactc aacgcaccag catcgccgta atcaaaattc gccccggcct
    10441 ccgcaccctg ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccag
    10501 ttggctgaat ataacctgcc ctcgccacac cggaccgtat ttaaatttgg ccgctgcttt
    10561 atacaggctt ataccgcttt tatcgccgga ccagtcttcg tcataggctt tattactttt
    10621 tgaaaacgcg atttcgttcg ggtgagagct gtcgccgttt tccgccattt caatcgccgt
    10681 aaacgcggca atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt
    10741 ggcgttccag gtggagtgag aaaggttggt tttgtattta tcgccgtcgg taacatcttt
    10801 acggtcgcgt tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcaatgaa
    10861 tccttcggcg cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcgat
    10921 agccagcgcc agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac
    10981 gtataaattg ctgcaccaca ggtgaacagc gaaaaaaata aaaattaaaa aagtagcgtt
    11041 gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat tatcaatctc
    11101 tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga tttttgttac
    11161 aaggcttaag tttgcggttg aaaggcttgc agaagaataa aagcgtgaaa ttccagcaag
    11221 atgaaagcgg ttacatttta tctaattatg tttt
//
LOCUS       DAZFTN010000141        11241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFTN010000141 DAZFTN010000000
VERSION     DAZFTN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(274..540)
                     /locus_tag="ACHKV2_004283"
     CDS             complement(274..540)
                     /locus_tag="ACHKV2_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO6942050.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(537..1304)
                     /gene="yjiL"
                     /locus_tag="ACHKV2_004284"
     CDS             complement(537..1304)
                     /gene="yjiL"
                     /locus_tag="ACHKV2_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO6942051.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            complement(1314..2465)
                     /locus_tag="ACHKV2_004285"
     CDS             complement(1314..2465)
                     /locus_tag="ACHKV2_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO6942052.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2581..3852)
                     /locus_tag="ACHKV2_004286"
     CDS             complement(2581..3852)
                     /locus_tag="ACHKV2_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO6942053.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3893..5125)
                     /gene="mdtM"
                     /locus_tag="ACHKV2_004287"
     CDS             complement(3893..5125)
                     /gene="mdtM"
                     /locus_tag="ACHKV2_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO6942054.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5592..6374
                     /locus_tag="ACHKV2_004288"
     CDS             5592..6374
                     /locus_tag="ACHKV2_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6942055.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDDCRANS"
     gene            6349..6549
                     /locus_tag="ACHKV2_004289"
     CDS             6349..6549
                     /locus_tag="ACHKV2_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942056.1"
                     /translation="MTIAERIHNDGWLLGREEGEQRILRLLLQNGADPEWIQKITGLS
                     AEQMQALRQPLPERERYSWLKS"
     gene            complement(6792..8204)
                     /locus_tag="ACHKV2_004290"
     CDS             complement(6792..8204)
                     /locus_tag="ACHKV2_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6942057.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8381..8545
                     /locus_tag="ACHKV2_004291"
     CDS             8381..8545
                     /locus_tag="ACHKV2_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO6942058.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8642..9523
                     /locus_tag="ACHKV2_004292"
     CDS             8642..9523
                     /locus_tag="ACHKV2_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO6942059.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            9571..10734
                     /locus_tag="ACHKV2_004293"
     CDS             9571..10734
                     /locus_tag="ACHKV2_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO6942060.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(10781..11122)
                     /gene="symE"
                     /locus_tag="ACHKV2_004294"
     CDS             complement(10781..11122)
                     /gene="symE"
                     /locus_tag="ACHKV2_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO6942061.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT         2940 a         2800 c         2838 g         2663 t
ORIGIN      
        1 aaaataatca gggggtgggg aaagataagc tttgcacaga aacttatctt ttaacattac
       61 aacacccggt aatcatttta tttctctatg catgattatg ggtttaagta taattcaatg
      121 tatattgtat tttagacgcg caataggaag gtgctatcaa aaatcgctca ttttgtggat
      181 gggaaatatt tcacggcagg cgagatattt actacaactt ccttcatgca ggattatgcg
      241 cagccaatta tccggttggt atatttaaag atcctattgc ctggttgacg cttctgcatc
      301 gtaatgcacc acgcactgcc agtcgctgcc ctgctggcaa tatatcgctt cggtttgccc
      361 agggatcacc cattgtattt cctcgccggg gggacaggtc agccaaatgc ataacccgca
      421 gccgccgcgt aaatcacggg gaatatcgct gacgcgaaaa gtcatgcccg ctgcctgcaa
      481 cgccttgcgg gtttgtatga cgccgaccgt ggagtgaaat aaaaataaaa actctttcat
      541 cggcgtgtcc tcactcgttg accaattacc gccgcgccaa ttgtgccagc aaattgcgca
      601 tcaggatggg tatttaccgg cattcgcagg tgagattcca gcatccgggc aaacgtctgg
      661 caatgactaa cgccaccagt aaacagaatc ggcgcttcac aggagagacg agcaatgaaa
      721 ttggcactcc gccgcgccat cgcgttaatc actccggcga gaatcgcttc tggcgcgacg
      781 cccgctgagc gcaggctgat cacttctgat tcagcaaaca ctgtgcacat actcgtgatg
      841 gcgtgcggcg tgacattttc ggtaatgcag tcgagttgct cgacgctggt gccaagcgtg
      901 cgcgagatca cctccaggaa acgcccggtg cccgccgcgc atttgtcatt catcaggaaa
      961 tcgcacaggt taccgtcatc atcaagctga atcactttgc tgtcctgacc accgatgtcg
     1021 attaccgcgc gcgttgctgg cgcaagaaac cgtgcgccca gcccgtgaca ggagatttcc
     1081 gtcacttgtt tatcggcaaa atccaccagt tgccgcccgt agccggtgag cgtcagaaac
     1141 ggcgttgtct ctaacccttc gcgcagagtt tcccaggctt cagtaattgc tgttgccggt
     1201 cgaaagggtg ttggaacgag gaaacggcgc gtaatcacgc cgtctgcgag taagatccct
     1261 ttggtggcgg tagaaccaga atcaatgcca atcgaatatg tcactgctac tccttacagc
     1321 atctcaataa aggccgcgac acgggtactg agctgcccaa catccgaggt ggagtagtct
     1381 gtttcaatag cgatataagg aatgttgtgc tgctgacgca catgacgttt aattgccagc
     1441 gattccaccg cgtaggtatg gcacgcctgc aaaatcacat caactacgcc atcgacctga
     1501 tattcttcca ccatctggct gagcattttc aggcgctgat cgttcggcga aacacaggag
     1561 cagccaatcg ccagatattt atccgccagc gcgtcgtaga catcgcccgt ttctgccacg
     1621 cattgctcgg tcgctttcgc cccggtgcag ttttcataac cgacaaccca gccgccattc
     1681 tcttcaatcg cgcgcaccac tttctctgct gcgccgccaa tcgggcagcc ggtgattaaa
     1741 atgcgcggac gcgggtccag tcgctggcct tcttcccact gctgacgaat gcgggcggtc
     1801 atcgcgtcca gttcattgat caacgcctct ttatcgaacc ggaaggttgc gccgtaaacc
     1861 actttcagaa tgtcgctgcc gctaagcgcc ggaggattga actgcccaag atgataaaaa
     1921 ttggccagtg cgcgacgttc gcggtttttc agcgcaatgg catcgcgcag agcatcttcg
     1981 ctaatttcgt gcccaaaacg ttcttccacc gctttttgta agcgcagcat ctcggctttc
     2041 cataacgcac gcgaggcatc gtctttaacg ctgtttggca gctgcatcac atgaacgggc
     2101 ttaaactccg ccatgtattc atacattttc tttttgccgt cgcaggtggt ttcaccgacc
     2161 accagatccg aaaagtagaa gtaggggcat ttatcggttt tgccgaagcc gtagctactt
     2221 ttaatcagcg ggcagaggtt gcgcggcaga tctttctccg cttcttcaat ggtttcatca
     2281 gaggtggaac agagcgaaac cacaaccgca ccggctgcca tcgggatctc ttgcggcata
     2341 aaggtgcagt aagtgccaac cagcggaatg ccgcgcacct tgagatccat gacggtgaga
     2401 aagcctttct ggcgagcttc agagaactga tcgaaaatgg cgggtagatc ggtgacaagt
     2461 gacatgattt tccttccccg taccacggga gataatgaaa agaggccaca ttattaccat
     2521 tcttttagta tggtttattt gatctctctc gcggttagcc acttagtttt tcatggattt
     2581 ctaaaaattg ccgaggggga acaaggccgg gagctgtgac agcaaatata aaattagccc
     2641 aatacaaccg ccaaccagcg taccgttgac acggataaac tgcagatctt tgccgatatt
     2701 taactctatt tgccgcgaca tatcccgggc atcccagctt tttaccgtat cgctgatatg
     2761 gcgcgtcagg aatgcagaaa actcaggcgc gacgcggtgc gcggcttgtt ccaggtgacc
     2821 atttaacgac gcccgcaagg catcatcggc aattaacgtt tcgccaaacc attgacctgc
     2881 ccgcgcgata cgttctttca cacgagaatc ttcactgttg atatccgctt tcagccattc
     2941 ccgtaaatcc ccccacaatt cactgagaaa ggcttcatcc tctttcagat agctttttac
     3001 ggcatcagct cgcgctgtca tttcaggatc gtttttcaac ttgtcgatca gggcaaaagt
     3061 ggcgcgatca aatgcatggc ggatctgatg cgcacgatcg cggctgatat catcaagcaa
     3121 agaattcacc gcgtcagaaa ccaactccgc gctatgttcg cccagccatt cggtggggag
     3181 aattttggct ttcagtggat gctcactctc cagccagcga acaatctgct gggcgataaa
     3241 cttgcgcgat ttatcgcgct ggagaagggc gatcaactgt gcgatcagcg tatccagcag
     3301 cacctgatga cgatcgtttt tggtcatact ctccagcatc aacgcactgg tgccggaaag
     3361 atcgacctta tcaatcgccc gatggaccgc gcgcttaagc aggcgctgaa tacgtgcatc
     3421 atcggtcagt tcaagaaaac cactcatgat ctgcaacaga tgctgaccaa cgcggcgggc
     3481 gttttctggc tggctaaacc agttgccaat cagcaacgcc ggttcgtgac gtcgaatcaa
     3541 tgccaccagc gattgggtat cgagaaattt tttctgcacg aactgaccga gattttcgcc
     3601 aatccggtct ttattacgcg ggataatcgc cgtatgacga gaaatgatcg gaatcggcac
     3661 gcggcgaaac agcgccacca ccgcaaacca gtccgccagc gcgccgacca tcgccgcttc
     3721 agcaatcgcc ttcacgccgc tcacccaaaa attgggcggc aaaaacagcg taacgacaaa
     3781 ggtagcagcg gcgataagca gtaaagagag cgccaacatt ttggcgcgtc tgagttcaat
     3841 gagtttattc ataacgttaa gaatagagcc tgacggcagg atcgcgcaaa aatcactgct
     3901 cctctgccag ctcggcagcc tgatgttggc gcacgcgatt aagcaatccg gccagggtga
     3961 aaacgacgat aacgcccgcc acaacggcta acagatgaaa cggcaagcga ccgccgttaa
     4021 accacagcca gcggccaatt tcgaccgaga cagacatcac catcaggatc accatattca
     4081 gcgatgcgga gacggtccct ttcggtaagt tattggaaaa cagcgtaaag cggaataagg
     4141 tcgggaagat caaaccaatc ccgaaagcat acagactggt gcccagcacc gaccacagcc
     4201 agacgtgcgg tgacagcaga ttgccgataa tcaacagcgc gaggccgacc agttgaatgg
     4261 gtacggcacg ccagataaag cgcggttcgg tcggatcttt aacgaaacgc gccacgatgg
     4321 cattcgcgac aatcaccgcg ccgaacaccg gaacctgtgt ccaggcgaac tgcgaggtcg
     4381 ttaagccgcc tgcgtcaatc aggatcaccg gcgagacagc cacccaactc atcatgggga
     4441 tatagcttaa agagatggtt gctgcgccaa agaggaacag ccgattgcaa aagacattac
     4501 gaaaatcgcg caagacgctt ttggcgctaa atggaaccgc gccgcgcttc accgtctctg
     4561 gcatcgccag cagtaagcca acaaatgaga taaaacccat aaccgcaatg atggcaaaaa
     4621 ggactttcca gtgcacaaag tgcatcagag ctgcgccgga aagcgggccg ataatcggcg
     4681 caatcagtac gatggaggtg ataatcgcca tcaacttgat cccttttgtc tggccgaacg
     4741 cctcctgcac cgtgacataa ccaacggtgg caataaaaca gatactggtg ccctgaattg
     4801 cacgcgcgat aagaaactgt gtcatagacg ttgtgaacat tgtcgcagcg caggcaaggg
     4861 taaatattag cgccccggta atcagcactg gcttgcggcc aatcctgtcg gaaagcggcc
     4921 ccagcagcca ctgcaacgcc atgcctccag cgagatagag actgacggca gccggagcca
     4981 gactgacatc ggcattaaaa tcacggacca cattaatgat cccaggctgg atcaggtccg
     5041 tcgaaagata ggcggcaaag tcatacaaaa tcaacgccat cgggaaaaac agcgtggcgg
     5101 catggcgggc aaaaaaacgt ggcataacaa acaactcctt gtccggagaa ccccaacggg
     5161 gaaaacgaaa ttcaataccg tcggtaatga caaaaggtag gcgagaagga caaatgtact
     5221 gaatttcatg taatcaacca cactgcctgt gaacacacag acagagaaat accttctgac
     5281 ttcgggtcag aatgtataac gattacaaat agtcatcagc acattacacc tcttgaattg
     5341 atgggttgca tagaaaatat acaccttaag tgtaattaaa atttgcgatt aatcaaaaga
     5401 gcgcggtgga agggggaata tctgccgggt acaggacatg taaaacgcga gtcagtttcc
     5461 ggaaaaatgt atcgtttgca gacgaacaaa aatagcctgg aaagcgcctc gggaaacgag
     5521 aaattgccgg gtgagaatgg ttttgttagt cgctaaagtc gggccatctt atcaacaggt
     5581 gacggatcgc catgacaaac ttcacgacca gcacgccgca tgacgcatta tttaaatctt
     5641 ttctcatgca ccctgacacc gcgcgggatt ttatggagat ccacttaccc aaagatttac
     5701 gtgaactgtg cgatctcgac agcttaaaac tggaatccgc cagctttgtc gatgaaaaat
     5761 tgcgggcgct acactccgat attctgtggt cggtaaagac ccgggaaggc gatggctata
     5821 tctatgtggt gattgaacat cagagccgtg aggatattca tatggccttt cgcctgatgc
     5881 gctattccat ggcagtgatg cagcgccata tagagcatga taaacgccgg ccactaccgc
     5941 tggtcatccc gatgctgttt tatcacggta gccgtagtcc ttatccctgg tctttgtgct
     6001 ggttggacga atttgccgac ccgactactg cacggaagct ttataacgcc gcgttcccgc
     6061 tggtggatat cactgtcgtg ccggacgacg agattgtgca gcaccgcaga gtcgccctgt
     6121 tggagttgat ccaaaagcat attcgccagc gcgatctgat ggggcttatc gatcaactgg
     6181 tagtattact ggttacagag tgtgctaatg acagccagat aactgcgctg ttaaattaca
     6241 ttttactgac tggcgatgaa gagcgtttta atgagtttat cagtgaacta accagtcgaa
     6301 tgcctcaaca cagagagcga ataatgacga ttgcagagcg aattcataat gacgattgca
     6361 gagcgaattc ataatgatgg atggctgttg ggaagggaag aaggggaaca gcgcattctt
     6421 cgattgttgt tgcagaatgg ggcagatcct gaatggatac agaagattac cggactttcg
     6481 gcagagcaaa tgcaggcatt aaggcagccc ttgcctgagc gtgagcgcta ttcatggctc
     6541 aagagctaac cagagacgga tgacaaacgc aaagcagcct gatgtgctac gcttatcagg
     6601 tctacaaagc ccattcaata tattgaactt taaagatttt gtagacctgg taaggcgttc
     6661 acatggcatc cggcatgaac aaagcgcact ttacctaagc catgccggaa cgtggctatg
     6721 atcctgcaaa gataaaacga gtcacaggcg attcgtgaga ataaatcagg tgcttcgcca
     6781 ctaattctaa tttattccat tgcccgatac acggcctcgc caatttgctt cagcgcttcg
     6841 cgataagttt cgctgagtgg caaagcacag ttgatgcgca ggcagttacg gtatttgccg
     6901 gaagctgaga aaatcgagcc tgccgccacc tggattttca tgcggcacag ctgccgcgtg
     6961 acgcagacca tatcgacctg ttcaggcaat tctatccaca gtaaaaatcc gcctttcggg
     7021 cgcgtaatac agatttcgca gggaaaatat tcccgtatcc agcaggtata aagcgccaaa
     7081 ttgcgctgat agatctgccg catccgccgg atatggcgat gatagtgacc ttccagcaca
     7141 aacgttgccg ccgccatttg cgtggacggc acattaaagc tgctgatggc gtatttcata
     7201 tgcatcagtt tatcgtgata acgccccggt gcgacccaac ccacgcgcag gcctggagca
     7261 atacttttac tgaacgagct gcacaacagc actcgcccgt cgatatccca ggaatgaatg
     7321 gtccgcgggc gcggatactc cgttgccagt tcgccgtaga catcatcttc aaaaatcaca
     7381 atatcatgac gctgagcgag agagagaacg gcccgtttgc gcgcgtccgg cataataaat
     7441 cccagcggat tattacagtt tggcaccaga atgatgcctt taatcggcca ctgttccagc
     7501 gccagttcca gcgcttcaac gctgatgcct gtttctggat cggttgggat ttcaatcact
     7561 ttcacgccca tgccgcgcag catctgcatt gaaccgtaat aacaggggga ttcgaccgcg
     7621 acaatatcgc ccggtttaca caccgccatt aacgctagcg acatcgagtt atggcagccg
     7681 ctggtgatga tgatgtcatc ggcagtgacc accgagccgc tgtcgagcat caggcgggca
     7741 acctgctcgc gcaatacccg ctgaccggct agcaagtcat aaccgagaac ggtttgcaga
     7801 ttatgctgca ccacccggct tagctcccgc cacagcggtt tcaggctggg cgtttcgaca
     7861 tccggcgtgc ttttgcttaa cggaacaatg gaactgtcgc tatgcgccac cagcatatcc
     7921 agcaccagat cccactgggt aatttccact gggcgctgca ccggacgcgt catcggcggc
     7981 actggcggct gggctttacg ttgtgcgaca aaataacccg aacgcggctg cggagtgatg
     8041 agcttcatcg tctccagcgt ctggtacgcc tgctgcacgg tgctgatgct gacgccgtgc
     8101 tcctgactta agctgcgcac cgacggcaat ttctccccgt gacgatacag cccttgctca
     8161 atccgctcgg caagtagagt cgccagatgt tgataacgcg tcatgctgta ttccttattt
     8221 ggaccataca gaaagaaaaa ccggtacaga tgaggctaaa aaaccggatg cagatgctgt
     8281 tttaacctgt ctgtatgtta aacaaaacta atatttgcat ctgtattgtg aactgccaga
     8341 agacggatga tgaagcctct gaaatatgag gaggcagcag atggaatttc acgaaaacaa
     8401 agccaaaacg ccgtttatca gacttgtgca actctggcag gcagtgaggc gttggcggcg
     8461 gcaaatgcag gcccgacggg tgttacagca gatgagtgat gaacggttga gggatattgg
     8521 gttacgcagg gaggatgtgg agtgaggggt atgttcgtcg cggataaaca ggatttctct
     8581 ataacctcca taaggaagtg gttattattg cgaaatcatc gtttcacgga aagacgtcag
     8641 aatgaaagct acagaagctc gcctactcga ttttttaaaa cgttcgcagc agtttgttat
     8701 tcccatttat caacggactt attcatggac agaacaacaa tgtcggcaac tttgggacga
     8761 catcattcgt gcaggaaagc gtgacgatat atcagcgcat tttatcggtt cggttgttta
     8821 tatagagcag ggattgtatc aggtttctgg tatttctccg ttgctggtca ttgatggtca
     8881 gcagcggctg acgaccgcaa tgttgctgat tgaggcttta tcgcgccatc ttggcgaaga
     8941 cgaagttttt gatggctttt cggcaatgaa attgcgtaat tattatttgc tcaatcctta
     9001 tgagtccggc gagaaaggtt ttaaattact gctgaccgag actgataaag acagtttact
     9061 ggcgttaata aaacaaagac caatgccaga aaactattcc catcgaataa tggaaaactt
     9121 tactttcttt gatgaacaaa ttgccaaact cggtgatgac ttgatcccct tatgtcgtgg
     9181 gttagcaaag ttattaattg tcgatgtggc gcttaatcgt ggtcaggata atccgcaact
     9241 gatttttgaa agtatgaact ccaccggtaa ggcgttaagt caggccgatc tggtgcgcaa
     9301 ttttattctg atgggcctcg aaccagagca tcaaacccgg ttgtatgaag atcactggcg
     9361 tccaatggaa gtcgcttgtg gtcagcaagg ttacagcgaa tattttgaca gttttatgcg
     9421 tcattatctg acggtaaaaa cggggagatc cctcggacag atgaagtcta tgaggcattt
     9481 aaactccatg cccgcagcca gagtgttgct gaaaaaggcg tagatcggct ggttgaagat
     9541 attcatatct acgcggagta ttactgtgca atggcattgg gaaaagaaag tgacaaatcg
     9601 cttgctacgg cttttcagga tttgcgcgag ttaaaggttg atgtggcgta tcctttctta
     9661 ctggcgcttt atcatgacta taaaaatgac gatttgtctc acgaagattt cctgagcata
     9721 attcgtttaa ttgaatctta tgttttccgc cgtgcagtat gtgcgattcc gacaaattct
     9781 cttaacaaga cgtttgctac tttttataag gtcattaata aagaaaaata tctggaaagt
     9841 attcaggtac attttttgaa tctaccttca tatcgtcgtt tccccaacga tgatgaattt
     9901 aaacgggaat taaaagttcg cgatctctat aacttccgta gtcgtagcta ctggttacga
     9961 cgactggaaa acgataaacg cagagagcgc gtggaagagt ttacgattga acatattatg
    10021 ccgcagaacg aaaatttgtc ggctaaatgg cgcgaagagc tgggaagtga ctggcagcgt
    10081 attcataaag aactgttgca tacgttgggg aatctcactt taacgcgcta taactcccgt
    10141 tacagtgaca gacctttcgc agaaaaacgc gatattgaag acggctttaa gcatagcccg
    10201 ctctatttga atatcggtct tggacagtgc gaaaaatggg atgaagctgc cattcacgcc
    10261 cgagccgatc gtctggcgga gcttgcggtt caggtctggc aagcgccttc tcttcctgaa
    10321 gaggttttag ctgtttatcg gggacagcct gagaacaaaa ccagttacag cctgagtgat
    10381 tatccttttc ttgctgatgg ttcgcatagc cgggtgttat tcgatcatct tcgcgatgaa
    10441 attatgcgcc tggacgcagg gatcacgcag gaagtattga agctctatat tgcgtttaaa
    10501 gcggaaacga attttgttga tgttgtgccg caaaaaagcc gactgcgatt gtcgcttaat
    10561 atgcagtttc atgaactggt cgatccgaaa ggtattgcca aagatgtgac aaatgttggg
    10621 cgatggggca atggtgatgt ggaaattggt ttcagtgacc tcgcacaact tccttacatt
    10681 atggggttaa ttcgtcaggc atttgaaaaa cagatggaga gcgcgttggt ataacgcagc
    10741 gaaaaatgcc cggtagtcct ggcgctaccg ggtcaatgta tcaggcaact ttctgtttac
    10801 ccgcaatcac cccaataaac tcctgcacct gcctttgttt acgcgccgac agcttgcaca
    10861 cctggcgcag cgactgcatc agttcgctct cggcggcggc gggtggttgg gcggtgagga
    10921 caatacagcc ttccatcact ttgacatcta ccaccgtgcc ggtggcaaaa ccggcggctt
    10981 ccagccactg acctttcagg gtgatggcgg gaatacggct gtaatccggg taacgactcg
    11041 cataactcac ggttaattga cggttatttg ccggggagac ttctgcttcg aacggttgtg
    11101 caatagaatg cgtatcagtc ataactgcta ttccctataa atagtgattg tgattagcgg
    11161 tgcgggtgtg ttggcgcaca tccgcaccgc gctaaatacc tggatatatc atcagtaaat
    11221 atggggaaag tccagctaaa a
//
LOCUS       DAZFTN010000142        11186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFTN010000142 DAZFTN010000000
VERSION     DAZFTN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..358
                     /locus_tag="ACHKV2_004295"
     CDS             32..358
                     /locus_tag="ACHKV2_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6942062.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            511..660
                     /locus_tag="ACHKV2_004296"
     CDS             511..660
                     /locus_tag="ACHKV2_004296"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942063.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            682..3216
                     /locus_tag="ACHKV2_004297"
     CDS             682..3216
                     /locus_tag="ACHKV2_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6942064.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            3228..4298
                     /locus_tag="ACHKV2_004298"
     CDS             3228..4298
                     /locus_tag="ACHKV2_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942065.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4326..5408
                     /locus_tag="ACHKV2_004299"
     CDS             4326..5408
                     /locus_tag="ACHKV2_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942066.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRLINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5620..6660
                     /gene="pstS"
                     /locus_tag="ACHKV2_004300"
     CDS             5620..6660
                     /gene="pstS"
                     /locus_tag="ACHKV2_004300"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO6942067.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6747..7706
                     /gene="pstC"
                     /locus_tag="ACHKV2_004301"
     CDS             6747..7706
                     /gene="pstC"
                     /locus_tag="ACHKV2_004301"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO6942068.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7706..8596
                     /gene="pstA"
                     /locus_tag="ACHKV2_004302"
     CDS             7706..8596
                     /gene="pstA"
                     /locus_tag="ACHKV2_004302"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO6942069.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8779..9552
                     /gene="pstB"
                     /locus_tag="ACHKV2_004303"
     CDS             8779..9552
                     /gene="pstB"
                     /locus_tag="ACHKV2_004303"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO6942070.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9567..10292
                     /gene="phoU"
                     /locus_tag="ACHKV2_004304"
     CDS             9567..10292
                     /gene="phoU"
                     /locus_tag="ACHKV2_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO6942071.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            10717..10851
                     /locus_tag="ACHKV2_004305"
     CDS             10717..10851
                     /locus_tag="ACHKV2_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942072.1"
                     /translation="MAISSYSTVTDKFANLTKMPDENNGLQKNQKNFVCLLVMRRSIQ
                     "
     gene            10818..>11186
                     /locus_tag="ACHKV2_004306"
     CDS             10818..>11186
                     /locus_tag="ACHKV2_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO6942073.1"
                     /translation="MSVGNEEKYSITHKDITNLIAGDRQSFLKLTLWEFILDLFPMYH
                     IKEAKETIVEFVTKTEDKVKRFERIKSLAIPEEKWRFSTTTEFTVDENKDIIVSRVFK
                     ININSKDNHNNAEENTMRSVL"
BASE COUNT         2948 a         2589 c         2774 g         2875 t
ORIGIN      
        1 ttttgctgtt actttgatta cgcagatgtc gatggctacg gctggtattg ttgttggggg
       61 aactcgcgtt attttttatg gtgacaaacc tgataccaca atctctattt ttaataaaga
      121 aacgaacctt ccttatctat tgcagacatg ggttgatcca tttaataaag aggataaaag
      181 caaaccgcca ttttctgtga tacctcccgt ttctcgcatt gaaccacagc aagagaaaat
      241 attacgcatt ttaaaaacgg ctggcgagtt accaaaagat cgggagtccg tattctggtt
      301 gaacgtgaaa aacatccctc cttcaattga taaaaaaata ccaatgttct tgagatagcc
      361 ataaaaacaa gaattaagtt attctggcga ccagccgcgg taaagataat ccctgagcag
      421 gctattaaaa atgttaaatg gtcgcggcag ggtaataatc tcatcgtcga aaatccaaat
      481 ccgatccata ttaatgtaat gagtgtgacg gtggatgata aagacgtgtc gctaaatatg
      541 atccgccctt ttgaaatatt gacattacca attccggctg gcgtggcagg aaaatcgctc
      601 gtttggcgct ttattaatga ttacggtgca attagtcaac cactaaagaa aaacctttaa
      661 gcgccggata aggatatatc gatggcctct tcgcatcttt tcattacgct tgcatcgggc
      721 atatgtctgc tctgttccat atctgctttt gcccgggata gcttgttcaa ccccagatta
      781 ctggaactgg atcatcctgc ggataatatt gatattcacc agttcaaccg ttcgaatacc
      841 ttacctgcgg gaacatacaa agttgatgtg atgatcaacg gcatgctctt cgaacgccag
      901 gaagttaaat tcgcccagga taaccctgat gctgaactcc acccatgcta cgtggcgata
      961 aaaaacgtgc tggcgaccta tggtataaaa gttgatgcga taaaatctct ggcgaatgtt
     1021 gatgacaaaa catgcgtaaa tccagttccg ctgatcgacg gggctacctg gttactggac
     1081 gccagtaaac ttgcattgaa tattactatt ccgcaaattt atctcaacaa tgcagttaat
     1141 ggttatatca gcccttcccg ttgggatcag gggatcaatg ccatgatgat gaattatgat
     1201 ttttcggcat cgcataccat ccggtcaaat tatgacgacg acgatgacag ttattatctg
     1261 aatttgcgta atggtattaa tttaggcgca tggcgttttc gtaattacag caccctgaat
     1321 tcttatgacg gtaatgtgga ctaccattcc gtcagtaatt acattcagcg cgacatcatg
     1381 gcattacgta gccagattat gattggcgat acctggacgg caagcgatgt atttgatagt
     1441 acacaggtgc gtggcgtgcg gctgtatacc gatgacgata tgttgccctc cagccagaac
     1501 ggctttgcgc cagtggtaca tgggattgcg aaaactaacg ccacggtgat catcaaacaa
     1561 aacggctacg ttatttatca atcagccgta ccacagggcg catttgccct caccgactta
     1621 aacacgacca gtagcggcgg cgatctcgat gtcactatca aagaagaaga tggcagcgag
     1681 cagcacttta ttcagccatt tacttcactg gccattctca agcgtgaagg tcagaccgat
     1741 gtagacctta gcattggaga agtgcgcgac gaaagcggct ttacgcctga ggtcttgcag
     1801 ttacaagcaa tgcacggttt ccctttggga ataactttgt atggcggaac acaattggca
     1861 aatgattacg cttctgccgc gctgggtatt ggtaaagata tgggggcgct gggcgcgatt
     1921 tcttttgacg tgactcatgc ccgctcgcag tttgactacg acgataatga gagtggtcaa
     1981 tcgtatcgtt ttctctattc caaacgtttt gaagacacca ataccacctt tcgtctggtg
     2041 ggttatcgct actctatgga ggggttctac accctcaatg aatgggtgtc gcgacaggat
     2101 aatgattctg atttctgggt aacgggcaac cgtcgcagcc gcttcgaagg cacctggacg
     2161 caatctttca cgccaggctg gggcaatatt tatttaacat tcagtcgaca ggaatactgg
     2221 cagaccgatg aggtcgaacg tttattacag ttcggctata acaacaactg gcgaaacatc
     2281 tcctggaacg tttcctggaa ctatacggac tcgatcaagc gctcattggg caaccatcat
     2341 gatgataaca atgatgattt cggcaaagaa cagattttca tgttctcaat gtcgataccg
     2401 ctatcgtgct ggatggaaga cagctacgtc aactattcgt taacgcaaaa caaccaccat
     2461 gaaagcacga tgcaggtcgg tctgaacgga acgatgctgg aagggcgtaa cctgtcttat
     2521 aacgtacagg aatcgtggat gcactctcct gatgactcct acagcggcaa tgccggaatg
     2581 acctatgacg ggacttatgg ctcggtcaat ggtagctatt cctggagccg tgactcccaa
     2641 cattttgatt atggcgccag aggcggcgtg ctggtgcata gtgacggagt gaccttctcg
     2701 caggaactgg gcgaaacggt ggcattggtc aaagcgccgg gcgcagaagg cctgtccatt
     2761 gaaaacgcca ccgggatttc taccgactgg cgtggttata ccgtaaaaac gcagcttagc
     2821 ccgtatgacg aaaaccgcgt ggcattgaac agcgactatt tctccaaagc caatattgaa
     2881 ctggaaaaca ccgtcatcaa cctggtacca acgcgcggtg cggtggtgaa agccgaattt
     2941 gtcacccatg tcggttatcg cgtgctattt aacgtccgcc aggtcaacgg taaaccaata
     3001 atgtttggcg cgatggcaac cgcctctctc gaaacgggca cagtcaccgg gattgtcggt
     3061 gataacggcg aactgtatct ctccgggatg cctgaaaaag gcgagttttt attgagttgg
     3121 ggacaagctg cggatgaaaa atgtaaggcg gcctatcaca tcacccataa acctgatgat
     3181 accagcctgg ttcaaatgga tgcgatttgt cgctaaagga agcatgaatg aaaaaaatat
     3241 tatcagggtt gattctgctg ctttgctgtc cttatggttt cgccgctaat ggtgatggcg
     3301 caacgcacat gtcaaattta tcatttggtc cgctgacggt ggcagcggcg aataaccact
     3361 ccggatacaa tattttcgag gcactgagca acacgactgg aacatacccg gtgcgctgtc
     3421 actgtgatga cacgcatggc ggaccgggcc aacaaacagc attttttcct atcttctaca
     3481 cgggagatgc cgcaccgggg cttgtgcttg agcgcactct taatgggtta aattactatg
     3541 ctctgaatga ttatttatcg gtcggcgtga cgatttttat tattaataac caatatgcgg
     3601 ccattccttt tgaacactta tccaaccaat ccacctcacc gcaacatacc tgtggagcag
     3661 gtaataatgg gagcactgta aatctggatt cagggcgctc ggcaaaatta tctttctatg
     3721 ttcggcattc tattactggc acggtgacaa tacccacaac ggaagtcgcc tggttgtacg
     3781 cgggcatgtc cgatcatttt cccaaaacga cccccgtttc taaagtgaca attcgcggac
     3841 aactaacggc cccgcagaac tgtgagttaa cgccaaatca gagcatcgat gtcgattttc
     3901 aaaaaattaa tagcgctgag ttctcctcaa cggcgggttc aattattgca gaaagaaaga
     3961 ttaaaaccga agtcacagta tcctgtaccg ggatggaaga cgtaaggtcc acggaggtgg
     4021 tgagtgcgtc gatgattgcg gcaaacagaa gtgccgatgc caccatgatc gtgacgagta
     4081 atccggatgt gggaattaag atttttgata agaacgaccg tccagtgaat gtggatgggg
     4141 gcaacttacc cgctgatatg ggtgctatta gtcgattagg aaaaaccgat ggtagcgtaa
     4201 cgttttattc agcgcccgcc agtctgacgg gcgcaaaacc agcgcctgat aatggattta
     4261 ccgctacagc cacgctggtt attgaattta ctaactaatc ttgtgttagt aaaaggaata
     4321 atttcatgaa aaaatggcat tatatatttt gcataattct ctttcattta gggttaccgt
     4381 gcgggtatgc ggcaaatgat ggcacgtgtg caacaagagg cggcacacat acattaagcc
     4441 ttaattttcc tctgacaacg gtcagtgcag caaacaatgt gcctggaaat acattaatag
     4501 atattgctaa tgcaacatct tctgaaaatt atagcgttct gtgtaactgt gattcaaaac
     4561 atagcaatgg cgcttatcac gaaatatatt ataccgcaga ccctgctccc ggtatggttt
     4621 atagcaccac cgcaagtggt cttgcttttt actatcttaa cgaatatgtc gatgtgggaa
     4681 caaaaatatc tgtgctaaat gcggggtata cggcagttcc ttttgaacat gtttccaacc
     4741 aggcaactac aacagatcac acttgtcagg gaaacaaaac tacagcggtt ggcgtgagcc
     4801 tgaaaactgg agcagatgcg aagatttcat ttcgtattaa acgtttaata aatggaacgg
     4861 tagtaatacc tatcaccgat attgcattgc tgtatgccaa catatccagc accacgaccc
     4921 gtggtgaggc gattgcaaaa gttcgaattt caggcagttt gaccgcacca cagtcttgtc
     4981 agataaatgc aggacaggtg atttattttg attttgatac tattcctgcg tccgaatttt
     5041 catctaccgc cgggcaagcc attacttcac gaaaaatcac taaaacagtg agtattgagt
     5101 gtacggggat ggggtatgag cgtacgcaga aagtcgatgc ttcttttacg gggacgaacc
     5161 gaagcagtga cgatacgatg gtggcgacag acaatgctga tgtcgggatc aaaatttaca
     5221 ataaatcgaa tgctgaagtt agcgtcaaca acggcaagtt acccgcagac atgggcaaca
     5281 cgaccatttt tggtcgtaaa aatggttcgg taactttttc ggcagcacct gccagcttta
     5341 ccggtgcccg gcctcagccc ggcgttttta acgctaccgc gaccttaacc attgaatttg
     5401 taaactaacc ttttgcggct gttttatgac agccgttcat atttcctctc ctttcatgaa
     5461 aaaatgctga tatttttccc cttcaatatc aataagtaat aactttcctt tgggtgtcat
     5521 caaactgtca cattccttac atataactgt cacctgtttg tcctattttg cttctcgtag
     5581 ccaacaaaca atgctttatg aatcctccca ggagacatta tgaaagttat gcgtaccacc
     5641 gtcgcaactg ttgtcgccgc gaccttatcg atgagtgctt tctctgtgtt tgcagaagca
     5701 agcctgacag gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg ggctgacact
     5761 taccagaaag aaaccggtaa taaagttaat taccagggta tcggttcttc cggtggcgta
     5821 aaacagatta tcgccaatac cgttgatttt ggtgcttctg acgcgccgct gtctgacgaa
     5881 aaactggctc aggaaggtct gttccagttc ccgaccgtga ttggcggcgt ggtgctggcg
     5941 gttaacattc caggtctgaa gtctggtgag ctggtgctgg atggtaaaac cctcggcgat
     6001 atctacctgg gcaaaatcaa gaagtgggat gatgaagcca tcgccaaatt gaatccgggt
     6061 ctgaaactgc cttcacagaa cattgctgta gtgcgccgcg cagatggctc cgggacttcc
     6121 ttcgtcttca ccagctacct ggcgaaagtg aatgaagagt ggaaaaacaa cgttggtact
     6181 ggctctaccg taaaatggcc gatcggtctg ggtggtaaag gtaacgacgg tatcgctgcg
     6241 ttcgttcagc gtctgccggg tgcaattggt tacgttgaat atgcttatgc gaagcaaaac
     6301 aacctggcgt acaccaaact gatctccgct gatggtaaac ctgttttgcc gaccgaagaa
     6361 aacttcgcta atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca ggatctgacc
     6421 aaccagaaag gcgaagatgc gtggcctatt acctctacca cgttcattct gatccacaaa
     6481 gatcagaaga aaccagaaca aggcacagaa gtgctgaaat tcttcgactg ggcgtacaaa
     6541 accggggcta aacaggcgaa cgacctggat tacgccagcc tgccggatag cgtagttgaa
     6601 caggttcgcg ctgcgtggaa gaccaatatt aaagacagta gcggcaagcc gctgtactaa
     6661 taaaactcca ggccggataa ggcattaaac ggcttatccg gcattacaaa atgactttgt
     6721 aaacgcgttt aactgaagag taacttatgg ctgcaaccaa gcctgctttt aacccaccgg
     6781 gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg attgtgctat
     6841 tgatgttggg tggcattatt gtctctctga tcatctcctc atggccgagc attcagaaat
     6901 ttggtctggc tttcctgtgg accaaagagt gggatgcgcc gaacgatatc tacggggcgc
     6961 tggtgccgat ctacggtacg ttggtgactt cgtttatcgc gctgctgatc gccgtcccgg
     7021 tgagtttcgg tatcgccctg ttcctgactg agcttgcgcc tggctggctg aaacgcccgc
     7081 tgggtatcgc cattgagctg ctggcagcca ttccaagtat cgtttacggc atgtggggcc
     7141 tgtttatctt tgcgccgctg ttcgccgttt acttccagga gccggtcggc aatatcatgt
     7201 cgaatatccc gattgtcggc gcgctgttct ctggcccggc atttggtatc ggtatcctcg
     7261 cggcaggcgt gatcctcgcc atcatgatta ttccgtacat tgcggcggta atgcgcgatg
     7321 tgttcgaaca aaccccggtg atgatgaaag agtcggccta cggtattggc tgcaccacct
     7381 gggaggttat ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc ggcggcatta
     7441 tgctggggct gggccgcgcg cttggtgaaa ccatggcggt gacctttatt atcggtaaca
     7501 cctaccagct cgacagcgcc tcgctgtaca tgccgggcaa cagtatcacc tctgcgctgg
     7561 cgaacgaatt tgccgaagcg gaatccggtc tgcacgttgc cgcactgatg gaactgggcc
     7621 tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg attatgcgcc
     7681 tggctaagaa tgagggggct cgctaatggc tatggttgaa atgcaaacca ctgcggcgct
     7741 ggcggaatcc cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta ttgcactgac
     7801 gctctcaatg gcgacgatgg ccttcggtct gttctggctg atctggattt taatgtccac
     7861 catcacccgc ggtatcgacg gtatgtcgct ggcgctgttc actgaaatga cgccgccgcc
     7921 caatacggaa ggtggtggtc tggcgaacgc tctggcaggt agcgggctgt taattttgtg
     7981 ggccacggta ttcggtacgc cgctgggcat tatggcgggg atttatctgg ctgaatatgg
     8041 tcgtaaatcc tggctggcag aagtgattcg cttcattaac gacattctgc tctctgcgcc
     8101 gtcgattgtg gttggtctgt ttgtttacac catcgtggtg gcgcagatgg agcacttctc
     8161 cggttgggcg ggtgtgattg cgctggcgtt gttgcaggtg ccgattgtta tccgcaccac
     8221 cgagaacatg ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg cgctgggtac
     8281 accgaagtgg aagatgatct ccgcgattac gctgaaagcg tcggtgtccg ggatcatgac
     8341 cggtatcctg ctggcgattg cccgtattgc tggtgaaacc gcgccgctgc tgtttaccgc
     8401 gctctccaac cagttctgga gcacggacat gatgcagccg atcgccaacc tgccggtgac
     8461 gatctttaag tttgcgatga gcccgtttgc ggaatggcag caactggcct gggccggggt
     8521 attgatcatt accctgtgcg tactgctgct gaacattctg gcgcgcgttg ttttcgcgaa
     8581 gaataaacac ggttgatatt gctgacacgg ttttcccctc accctaaccc tctccccaga
     8641 ggggcgaggg gaccgaccga gcgccttttt gactctgtac acggttaaca ctttgccgga
     8701 tgcggcgtga acgcctgatc cggcctacgg taagcctgat tcgcgaagtg catcaggcac
     8761 gatgaggaaa agattgcaat gagtatggtt gaaactgccc cgagtaaaat tcaggttcgt
     8821 aatttgaact tctactacgg caaattccat gccctgaaaa acatcaacct ggatatcgcc
     8881 aaaaaccagg taacggcgtt tatcgggccg tccggctgcg gtaaatcgac gctgctgcgt
     8941 accttcaaca aaatgtttga actgtacccg gaacagcgtg cggaaggtga aattctgctc
     9001 gatggcgaca acatcctgac caactctcag gatatcgccc tgctgcgtgc gaaagtgggc
     9061 atggtgttcc agaaaccgac gccgttcccg atgtccatct acgacaacat cgcctttggc
     9121 gttcgtctgt ttgagaagct ctctcgtgcc gacatggacg agcgcgtgca gtgggcattg
     9181 accaaagccg cattgtggaa cgaaaccaaa gataaattgc atcagagcgg ttactctctc
     9241 tctggtggtc agcaacagcg tctgtgcatt gcgcgtggta tcgccattcg cccggaagtg
     9301 ctgctgctcg acgaaccatg ttcggcgctc gaccccatct ccaccgggcg tattgaagag
     9361 ctgatcaccg aactgaagca ggattacacc gtggtgatcg tcacccacaa catgcagcag
     9421 gctgcgcgtt gttccgacca cacggcgttt atgtacctgg gcgaattgat tgagttcagc
     9481 aacacggacg atctgttcac caagccagcg aagaaacaaa cagaagacta catcaccggt
     9541 cgttacggtt gattcaggag tgcgttatgg acagtctcaa tcttaataaa catatttccg
     9601 gccagttcaa cgccgaactg gaaagtatcc gcacgcaggt gatgaccatg ggcggcatgg
     9661 tggagcagca gctttctgat gcaatcaccg cgatgcataa ccaggacagc gatctggcga
     9721 agcgcgtgat cgaaggcgac aagaacgtca acatgatgga agtggcgatc gatgaagcct
     9781 gcgtgcgcat tattgccaaa cgccagccga cggcgagcga cctgcgattg gttatggtga
     9841 tcagtaaaac cattgccgaa ctggagcgta ttggcgacgt ggcggacaaa atctgccgta
     9901 ctgcgctgga gaaattctcc cagcagcatc agccgttgct ggtaagtttg gagtcgctgg
     9961 gccgtcatac tatccagatg ctgcacgacg tgctggacgc gttcgcgcgg atggacattg
    10021 acgaagcggt acgtatttat cgtgaagata aaaaagtcga tcaggagtac gaaggtattg
    10081 ttcgtcaact gatgacctac atgatggaag attcgcgcac cattccgagt gtacttaccg
    10141 cgctgttctg tgcgcgttct atcgaacgta ttggcgaccg ctgccagaat atttgtgagt
    10201 ttatcttcta ctacgtgaag ggtcaggatt tccgtcatgt aggcggtgat gagctggata
    10261 aactgctggc gggaaaagac cccaaagagt agtgctaact gtaggccaga taaagctttt
    10321 tgccgcatct ggcaatcagc ccggtcatac atagttaccg ggctgataca ctttctcact
    10381 gaaatattgc cacacaaact cacctcgaaa ctctccccag tacattgaat atacattatt
    10441 tcatttgaat tattttactt ttaatgtagt gcctcgctat aaaaacatat ttgttattct
    10501 tttcatttat atcgctacct gcacagagaa tggaaaagaa aacaaaaaac agggtaaaag
    10561 ccctatatcg agacataaaa aatgagcgat tttttacagt attcataggt ggtttagtca
    10621 taatcgtagc aaaccaacac ccaggtatgc tttttaagac aaatcggcaa tgtattatgc
    10681 tcgggcgaat aaatattgtg ttcaggagaa atgattatgg ctatatcatc atatagtaca
    10741 gttacagata aatttgctaa tctgactaaa atgccagatg aaaataatgg attgcaaaaa
    10801 aatcagaaaa attttgtttg tctgttggta atgaggagaa gtattcaata acccataaag
    10861 atataacaaa ccttatagct ggtgatagac aatcttttct taaactaact ctttgggaat
    10921 ttatactcga tctctttccc atgtatcata ttaaggaggc taaagaaaca attgtagaat
    10981 ttgtaacgaa aactgaggac aaagttaaga gatttgagag aattaaatct ctcgctattc
    11041 cagaagaaaa atggcgcttt agtacgacga cagaatttac agtagatgag aataaagaca
    11101 ttattgttag tagagtattt aaaataaata ttaactcgaa agataatcat aataatgctg
    11161 aagaaaatac aatgcgttct gtatta
//
LOCUS       DAZFTN010000143        11175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFTN010000143 DAZFTN010000000
VERSION     DAZFTN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..810
                     /gene="csgD"
                     /locus_tag="ACHKV2_004307"
     CDS             160..810
                     /gene="csgD"
                     /locus_tag="ACHKV2_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO6942074.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            815..1204
                     /gene="csgE"
                     /locus_tag="ACHKV2_004308"
     CDS             815..1204
                     /gene="csgE"
                     /locus_tag="ACHKV2_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO6942075.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1229..1645
                     /gene="csgF"
                     /locus_tag="ACHKV2_004309"
     CDS             1229..1645
                     /gene="csgF"
                     /locus_tag="ACHKV2_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO6942076.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1672..2505
                     /gene="csgG"
                     /locus_tag="ACHKV2_004310"
     CDS             1672..2505
                     /gene="csgG"
                     /locus_tag="ACHKV2_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO6942077.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2569..3060)
                     /gene="ycdZ"
                     /locus_tag="ACHKV2_004311"
     CDS             complement(2569..3060)
                     /gene="ycdZ"
                     /locus_tag="ACHKV2_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6942078.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3162..3716)
                     /gene="ycdY"
                     /locus_tag="ACHKV2_004312"
     CDS             complement(3162..3716)
                     /gene="ycdY"
                     /locus_tag="ACHKV2_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO6942079.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3740..4477)
                     /gene="ycdX"
                     /locus_tag="ACHKV2_004313"
     CDS             complement(3740..4477)
                     /gene="ycdX"
                     /locus_tag="ACHKV2_004313"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO6942080.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4532..5470)
                     /gene="ghrA"
                     /locus_tag="ACHKV2_004314"
     CDS             complement(4532..5470)
                     /gene="ghrA"
                     /locus_tag="ACHKV2_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO6942081.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5704..5791
                     /locus_tag="ACHKV2_004315"
     tRNA            5704..5791
                     /locus_tag="ACHKV2_004315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5738..5740,aa:Ser,seq:gga)
     gene            complement(5941..6783)
                     /locus_tag="ACHKV2_004316"
     CDS             complement(5941..6783)
                     /locus_tag="ACHKV2_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6942082.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6868..7065)
                     /locus_tag="ACHKV2_004317"
     CDS             complement(6868..7065)
                     /locus_tag="ACHKV2_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6942083.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7077..7568)
                     /locus_tag="ACHKV2_004318"
     CDS             complement(7077..7568)
                     /locus_tag="ACHKV2_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6942084.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7565..7939)
                     /locus_tag="ACHKV2_004319"
     CDS             complement(7565..7939)
                     /locus_tag="ACHKV2_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6942085.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7986..8360)
                     /locus_tag="ACHKV2_004320"
     CDS             complement(7986..8360)
                     /locus_tag="ACHKV2_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6942086.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8523..8744)
                     /locus_tag="ACHKV2_004321"
     CDS             complement(8523..8744)
                     /locus_tag="ACHKV2_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6942087.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8807..9283)
                     /locus_tag="ACHKV2_004322"
     CDS             complement(8807..9283)
                     /locus_tag="ACHKV2_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6942088.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9299..9784)
                     /locus_tag="ACHKV2_004323"
     CDS             complement(9299..9784)
                     /locus_tag="ACHKV2_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6942089.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9875..10693)
                     /locus_tag="ACHKV2_004324"
     CDS             complement(9875..10693)
                     /locus_tag="ACHKV2_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6942090.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11033..>11175)
                     /locus_tag="ACHKV2_004325"
     CDS             complement(11033..>11175)
                     /locus_tag="ACHKV2_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942091.1"
                     /translation="MLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAMAR
                     IK"
BASE COUNT         2815 a         2797 c         2775 g         2788 t
ORIGIN      
        1 tagattgaaa tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat
       61 tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc
      121 tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt
      181 attcatggtc atacattatt gttgatcact aaaccttctt tgcaggcgac agctctcttg
      241 cagcacctta aacaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg
      301 gacgatatct cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
      361 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      421 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      481 ttttatgcca tggaggatca agaacgcgtt gtcaatggat tgcaaggcgt cctgcgcggc
      541 gaatgctact ttacgcaaaa gcttgccagt tacctgatta cacattctgg taactatcgt
      601 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataagctg
      661 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      721 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      781 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      841 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      901 attgttaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      961 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
     1021 gggaagctgg atcacaataa cggtcaatca ggacgttatt ttccagactt ttttattccc
     1081 gttgaaaaga gacttcgaaa aaactgtcgt ctttgcactg attcaaactg aagaagcact
     1141 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
     1201 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
     1261 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1321 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1381 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1441 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1501 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1561 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1621 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1681 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1741 gccgccagac cgacattaat gcctcgtgct cagagctaca aagatttgac ccatctgcca
     1801 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1861 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcgatgctg
     1921 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgccaggg cttacaaaac
     1981 ctgcttaatg agcgcaagat tattcgtgcg gcacaagaaa acggcacggt tgccattaat
     2041 aaccgaatcc cgctgcaatc tttaactgcg gcaaatatca tggttgaagg ttcgattatc
     2101 ggttatgaaa gcaatgtcaa atctggcggg gttggggcaa gatattttgg gatcggtgcc
     2161 gacacgcaat accagctcga tcagattgcc gtgaacctgc gcgtcgtcaa tgtgagtacc
     2221 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     2281 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctcg
     2341 aacgaacccg ttatgctgtg cctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2401 aatgatggta ttgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2461 attctggtga aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaatgccg
     2521 gaattaataa tccggcattt actgtttaag cgaaagcgtg agtgcccctc acgctttatt
     2581 tttgatttcc tgctcacgga gagccgtctt tgcactacgc gccgccagcc acaggccgct
     2641 gtttttcatt gcgtaaccaa atatcagccc aagcgccagc gaaggtaaca ccagtttcca
     2701 gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag
     2761 cagcagctgt ttggcctgaa tacacatcag aaacgcgaca ataccggtta tgacataact
     2821 gagaatttcc agatgtggcg ccagtgcact accgtaaata atgaccatcg cccacaccac
     2881 gccactaagc agagttgcag cggagatcgc cagccctttc agcccacctt gcgggcaggc
     2941 aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac
     3001 ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat
     3061 gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac
     3121 agcaaattat ctgttgcagt tatgagtgac gcatatcaca tttactcttc agaatcttct
     3181 tccagctcgt cccacattgc actaatggca tcgcgggtta gcggtgccat ggtgcgccag
     3241 aaaggcgtgg ttgcatgggc ctccactttg ccaaggaacg caccacacca gggtaacaga
     3301 tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc
     3361 caggaagccg caagcagcaa tgtgccgatg tgatcggcag gcgtatccgc taatggcatc
     3421 cctcgttcag aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta
     3481 cgatatggcg gcacggcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact
     3541 tgcgtcatat cacagctttt ctgcaaacgt gtcagcaact catcctgctc cagtggccag
     3601 ttcgcagcca gtttcccctc acgaatcagg gtaaacagcg gcaccagtaa aggatcttgc
     3661 ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat
     3721 atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag
     3781 attcaaggaa gttcagtaag cggcgcggag aaacattcaa aatgcgctct ggcggaaaat
     3841 ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac gcggtgtgag
     3901 aatccgagcc taatgccacc caacctccag catcacgtac cgctgcggct accgcacgac
     3961 agttgtcttc actgccctta cgtgagtgta aaaatgagga attattgatt tccagcgcca
     4021 cctgatgttt cgcggctgct tcagcaacgg ctttaacatc tatctcatat ttcggatttc
     4081 cgggatggct tattatatgc acattgccgc tggcgatagt ggcgatcatc gcttgtgtat
     4141 tggtcgcttt gtcatgtggt gcaaaaaccg gctcatgaaa accggcaata atcagatcca
     4201 gcgagtcaaa cattttaccg ctgcaatcaa tttcaccatc aacattttta atgttagctt
     4261 cgatgccgcg caggatccct accccatcaa ccactcgcgg ccagatacgc atgttaatga
     4321 agtgccagtg atgcggcgca tcttccatgt ccgggccatg atcggtaatc gcaaaaagtt
     4381 taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc
     4441 tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc
     4501 cgccaaggat agcaggaatc ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc
     4561 cgcagaccct ctcccctttt tcgagctggg caatagtgcg agaaatgtac tccacagctt
     4621 cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga
     4681 gcggactttc aggcggtaag ggttcacgat taaaaacatc caacattgcg cctttaactt
     4741 tgccgctatc cagcgccgcg agcaggtcat cttccacgac atgaacacca cgcgccaggt
     4801 tgaggagata cgcgccatcc ggtaattttt cgagtaattg ttgattaata atgccgacgg
     4861 tttcaggggt attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca
     4921 gttcttccca tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc
     4981 aacgcagcgg aaagcgccag gtttgcagac tctgagcaac tttactgccc agtacgcctg
     5041 cgcccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg
     5101 aactattttg ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga
     5161 cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag
     5221 aaggcttcag catttcaggg tgtgcctgta gcttgctcaa aatagaatca acaccggccc
     5281 cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat ttcaacagga ggatgccagg
     5341 ctaaagcata atcagcagag tcattatctc cgcttttcca tgctctgact cttgcctgag
     5401 gaatagcttt gcgcagtgcc tctatccacc attgggtatc gaacgttggg tgataaaaga
     5461 tgatatccat actgactccc gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg
     5521 atctacaaag accaccagca taacaaatcc gtggtcggtg gccaaaaaag cagattttgc
     5581 ttattaaaac tgcatattga ttgaagtttg aataaacgcg cgatttttta aaaaagtttg
     5641 ttgacctcag gtcatgattt ccctaaatta gcgcccgttc cagcaagaca ggaacgacaa
     5701 tttggtgagg tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat acggcaacgt
     5761 atcgggggtt cgaatccccc cctcaccgcc agattataag cgcatgttat tgtttaacat
     5821 gcgctttttt attttccctt ccagacagta tgccatcggg tatgccatct gaaaccggtt
     5881 tatatgcccc gcgatcatat cttatcggac aatttccgat ctttgagcat actggcaaag
     5941 ctatactgcc agcatatcag gatagtgttt tgcgataata tcattcagtc tgtcaaccag
     6001 ttctggtttt ctgaacgtga tatgcgcgga gcccttctta aagtatctga tgctgaacat
     6061 ctcatcttca tagctctctt taccctgaac agactgaatg tgatcatcca gacgaagggt
     6121 gatgtttccc cggttgtcgg ggattggttt accgctgaac aggtgcaaca ttctttccag
     6181 atcggcgagt cggtcagcct gttgtccggt gataagatga aatccccacc ggtcccatct
     6241 caccagattg ttgacgataa ttttactgcc aaatttacag gggcaattgg ttttgtaatt
     6301 ccagttcagc cctctgaaga cgttgatcac ccctcgctca aacacctcat ccttattctg
     6361 gtgcagttgt tcaaatgtgc tcagaatgtt cgcttcgctg atttccggaa aattatcgta
     6421 ctccagtgac cggtaccatg tatcacgggc ctgcgtatcc attaatgaca gcatcccgga
     6481 acgttgcatc aggtcacgcc agatttcgcg atcaagattc cgggtaatgg ctttcattgc
     6541 tgtttcgggt ttctccatca gccagcagcc gcagcgaaaa tcctgtttca tggcccagtc
     6601 cggggcagtt ttacctccga tgctttccgt cagcaccgag atttcagcca gttgtcggat
     6661 aagtgtgtcg atttgcaaca gtgtactctt tcgtacagcc agaatgtggc gaatgctggt
     6721 tgagcagata acgtccgtat gcccgttgac cacttccgga cgggagattg tgatatctga
     6781 cataatcgat attccttttt gcagataaat gaaaacgcta gccctgtgca ggctggcgtc
     6841 agaggaatga agtctggtga gtgagcttca ccggttctgg tgcagaagct gcagatgacg
     6901 cagggtacgg acatcctccc gggcctgttc gatacagggc aacagtgctg ctgaatccgt
     6961 tttatcctca tcgttgtcaa agataattcc cgatccgcag tcgatattgt cctgcagcca
     7021 cgcaatcaga atatccagcg ctgtttccgt ggttaatgat ttcatgttgt tcctggttac
     7081 cagtcgaaag tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg
     7141 acgccataac gccggatgag tgtgacgacc agacgacgga acgtttttga cagacccagg
     7201 cgtttaagcc gcagaaccgg gtacgaccac gcatcggtac gcaacagata gccgttaccg
     7261 gtaaaatgaa tccattctga ttcaccgaag tcactggtct ggtgtgacag cgagtacagc
     7321 caggcgttgt ccgtttccgt gatatgtgcg gtactgcagc gtatgccggt aagggtagca
     7381 aatggtggcg tgcattctgc tgaggtctgg tcagattcat caacaacacg aagggagtag
     7441 ccgttatcgc agaccatgtt aatcagttca gcgagctcaa taccatcgac gtcaacgaca
     7501 atgcgcccca tgttcagtgc ctgtacgtta acgctgtcgg cttccagtgt cagggccagt
     7561 ttcatcattt cgcctccgga tacttaccca gggtaatgtt atttaccgtt ctgtaattgt
     7621 cgcgggtcat caggccggtc gcccggcgag cccggaggat atcaatactg tttattaact
     7681 gagagcgggg acaggtgctg aatcccggct ggtcggtacg caccagcgcg tatttttcca
     7741 cgagaaaatt aactgcatca cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac
     7801 gttcatcggc aaacggtgtg tcattcagtg tgaggccata atgctggtcc agcagtcgga
     7861 tgagcagtgt ctgccagatg gtgacgggag acgggcagcg agatacctcc cgtacatgtg
     7921 tgtcggataa tgttttcatc ttaagttctc tgtaactggt agtgctaaag gtgagagctt
     7981 cggggttatg aggtggttgc gggtgctggt gtcggataaa cagccagata aacgtaacca
     8041 caggagccga gggtgtcggc ttcgcaggtc agcccttttg cattcagcgt gacggtatgc
     8101 tgatatcggg gattcagttc accgctggtg agcatgagtt ccagttgttt catcagcagc
     8161 ggaaaggcct ggtccagatg gtatgcatct gcgtcgctga agcggcctct gataccggcg
     8221 cggtcggcaa ggtaatgcag gcggttaccc tcttgcacca gacgagcgcc aaaacagggc
     8281 ctcactgtgc agggaagccc ccaccagggg cggtcgtgat tgtcatcggg atgagttatc
     8341 ccggagagtt tgtctgacac gataaaatcc tctctgaaaa gtggttaaga atggtttaac
     8401 gatgacaggt cagccggcgc gctgaccggt ttcggtgaag atgtatgcat tgatgagaat
     8461 ggcctcatcc acagcggcgt ctgaggtcat ctggtcgtac tcgtttcccg ggtgacggaa
     8521 catcagttca gggtgacact catcagccgg acatacgggc caaaactgtc cttacggcgt
     8581 tctgcaaaca cggcaagaac gccgggaata tcctgcactt cacgaccagt atacgcttca
     8641 gcactgccgt gccagcgata cttaccggta cagaacggaa aaagacggga tgcaggatgc
     8701 tgttggtgaa tacgcatggc ttcaccacgg gtgatgattc tcatgatgtg atacctctga
     8761 agacagaaga taaaagtgaa aacaggtgtg atgtggttgt gacgggttaa agcagaccat
     8821 gttcggcaaa ggagaaaacc tggttgccac cgactatcag atggtccggc acccggatat
     8881 ccaccaggcc cagtgcctgt accagacgtt cggtgataag ccggtctgcc ttactgggtg
     8941 tgacttcacc ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg
     9001 cgcgtttaat cacttcccgg ggatgaactt ccgtacggtt gatggtgccg gtgaagaggg
     9061 tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaac tcttcccgct
     9121 ccagtcccgc catgttcaga atcagccatt cccgtgccgc acgggtggag gtgaaggcca
     9181 cgccgggttc atgaagatgg cggtccaggg tttgcagggc ccgcagaatg agactgcgct
     9241 cgccgggcgt catctctccg ggcagaaagg aaagctgctg cattgtccct gcctccattc
     9301 agtcaatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc
     9361 ggtaatagtg gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca
     9421 tcagacaggc agcaataccg gcggcttccg ggctcatttc agcgcggttg ccgttcatgg
     9481 cattgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc
     9541 cgccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttcg caaagacggt
     9601 ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaaatgct
     9661 gcggccagaa gctgatgcgc tgttcatcag ggacgggagt ggcaaccagc tgaggtgggt
     9721 tattccctga tgcagtggtt acggaaacag aaggtgtggt ggaattatgc gaaacggttg
     9781 tcatgaggtt attccttata aaaagtaaat gaatggaaga agccccgggg caggaacaga
     9841 tgtgagtcag gactgcactt tcagtgaaac ggcatcagcg catgctctcc agcagcgttt
     9901 ctgccatcac ccacaatgcg cgattgagct taatgtcggt atcaatgctg tgaatggccc
     9961 gggtatggat acgttttcct tttgcactgc gaccggaaat cccgcctttc agcatattct
    10021 cctggatggt ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggtg
    10081 tcagaatgtc ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagtgccg
    10141 cctgtgccag cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct
    10201 tctcctctat ccggtcaaaa acgcccacca cctcgtaagc cccttcaatg actctgtcca
    10261 ccacatttcc ccggtgtgga acacgcaatt ctcccagaga ctgaccgcag accagtccat
    10321 tggtacaaat ggcacgaaaa tatccgggta acatctggta gctggatgaa ccgtcatggg
    10381 agttgagcag aataatttca gggacatgct gaccggttat ctgtccggcc cgccgcagac
    10441 gcagcatgtg ttttgtgtat tcccggcggc tctggtcgcg cacgcgggtc tggcaggcaa
    10501 agaacggctg aaagccttcc tgctgcagat tttccaggac ggtgatagtg ggaatgtacg
    10561 catagtgttc actgcgagag gtatgtcggt tttccccaaa aatactcggt acatggcgca
    10621 tcagttcttc gtgtgtcagc ggacgatcac gacgtatctg gttcgcataa ccaaaacgac
    10681 tggctaatct catgatttgc tccttatgaa tgattaagcg atataaatga aaaagccgcg
    10741 tctcccggag aagacgcggc ctgatgggtg aaatgaatga ggtttattgt ctgagaagcc
    10801 cttcactggc gaaccacgta ttaagctgta ttccggcatc gccagtgcaa ctgaccttca
    10861 gcattgcgga taacccgccg gaaatatgtt cactggttgt ttttgtatct tcccccaaaa
    10921 ttaaataaat tcatcttaac catcacccag ataatatata tctgaaaata ttttcaataa
    10981 caccagacag gaaaaacacc accacaggcg gtggcgtttc tttggggcgt ctttatttaa
    11041 tccgggccat cgcgtttctc agtgccaaat ctgcctcttc cgttatatcc aggcttttct
    11101 gcaataccgc ctctgaccgg gtcagtgcct gacgtgctgc cagcggtttc cggatatgct
    11161 ccatcgccag catcg
//
LOCUS       DAZFTN010000144        11155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFTN010000144 DAZFTN010000000
VERSION     DAZFTN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(193..2022)
                     /gene="glmS"
                     /locus_tag="ACHKV2_004326"
     CDS             complement(193..2022)
                     /gene="glmS"
                     /locus_tag="ACHKV2_004326"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO6942092.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2184..3554)
                     /gene="glmU"
                     /locus_tag="ACHKV2_004327"
     CDS             complement(2184..3554)
                     /gene="glmU"
                     /locus_tag="ACHKV2_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO6942093.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3906..4325)
                     /locus_tag="ACHKV2_004328"
     CDS             complement(3906..4325)
                     /locus_tag="ACHKV2_004328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO6942094.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4346..5728)
                     /gene="atpD"
                     /locus_tag="ACHKV2_004329"
     CDS             complement(4346..5728)
                     /gene="atpD"
                     /locus_tag="ACHKV2_004329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO6942095.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5755..6618)
                     /gene="atpG"
                     /locus_tag="ACHKV2_004330"
     CDS             complement(5755..6618)
                     /gene="atpG"
                     /locus_tag="ACHKV2_004330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO6942096.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6669..8210)
                     /gene="atpA"
                     /locus_tag="ACHKV2_004331"
     CDS             complement(6669..8210)
                     /gene="atpA"
                     /locus_tag="ACHKV2_004331"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO6942097.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8223..8756)
                     /gene="atpH"
                     /locus_tag="ACHKV2_004332"
     CDS             complement(8223..8756)
                     /gene="atpH"
                     /locus_tag="ACHKV2_004332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO6942098.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8771..9241)
                     /gene="atpF"
                     /locus_tag="ACHKV2_004333"
     CDS             complement(8771..9241)
                     /gene="atpF"
                     /locus_tag="ACHKV2_004333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO6942099.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9303..9542)
                     /gene="atpE"
                     /locus_tag="ACHKV2_004334"
     CDS             complement(9303..9542)
                     /gene="atpE"
                     /locus_tag="ACHKV2_004334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO6942100.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9589..10404)
                     /gene="atpB"
                     /locus_tag="ACHKV2_004335"
     CDS             complement(9589..10404)
                     /gene="atpB"
                     /locus_tag="ACHKV2_004335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO6942101.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10413..10793)
                     /gene="atpI"
                     /locus_tag="ACHKV2_004336"
     CDS             complement(10413..10793)
                     /gene="atpI"
                     /locus_tag="ACHKV2_004336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO6942102.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2678 a         3028 c         2762 g         2687 t
ORIGIN      
        1 acaaagtaca tcaattatgg caattgtttg tcaatgattc gaggtattta attactacta
       61 cggttaaaac aagtgttcaa cggtgcccca aataatagct gaagttaatt atatggaatg
      121 aggttagatt attaatgctg gtaagtaaaa gggccgccaa aaggcagccc ttcgttcagc
      181 aaatgaataa tattactcaa ccgttaccga ttttgccagg ttacgcggct ggtcaacgtc
      241 ggtgcctttg atcagcgcga cgtgataagc cagtaactgc agcggaacgg tgtagaagat
      301 cggtgcaatc acctcttcca catgcggcat ctcgatgatg tgcatgttat cgctacttac
      361 aaaacccgca tcctgctcgg cgaagacata caattggccg ccacgcgcgc gaacttcttc
      421 aatgttggat ttcagttttt ccagcaattc gttgttcggc gcaacgacga taaccggcat
      481 atcggcgtca atcagcgcca gcggaccgtg tttcagttcg cctgcagcgt aggcttcagc
      541 gtgaatataa gagatctctt tcagcttcaa tgcgccttcc agcgcgattg ggtactgatc
      601 gccacggccc aggaacagcg cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat
      661 gcgtttgtcc tgagacagca tctgctcaat acggctcggc agcgcctgca gaccatgcac
      721 aatgtcatgt tcaatggagg catccagacc tttcaggcga gacagcttcg ccaccagcat
      781 caacagcaca gttaactgag tggtgaatgc tttagtggat gccacgccga tttccgtacc
      841 cgcgttggtc atcagcgcca gatcggattc gcgcaccaga gaagaacccg gaacgttaca
      901 gattgccagc gaaccaaggt aacccagctc tttcgacaga cgcaggcccg ccagggtatc
      961 cgcggtttcg ccagactgtg acaaggtgat catcaggctg ttacgacgca cggcagattt
     1021 acgatagcgg aattcagagg cgatttcgac gtcgcacgga atacctgcca gcgattcaaa
     1081 ccagtaacgg gaaaccatac cggagttata agaagtacca caggcgagga tctgaatatg
     1141 ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc
     1201 gtggctgatg cgcccggtaa gggtgttttt gatcgcgttc ggctgttcgt agatctcttt
     1261 ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca tattgcagat tggattcgat
     1321 atcctgacgt tttacttccg cgccagtttt atcgaagatg tttaccgaac ggcgagtgat
     1381 ttccgcaata tcgccctctt caaggaagat aaagcgacgg gtcaccggca gcagcgccag
     1441 ctggtcagaa gcgataaagt tttcacccat acccagacca atcaccagcg gactaccaga
     1501 acgtgctgcc agcagggtat ccgggtgacg ggagtccatg atcactgtac cgtacgcacc
     1561 acgcagttgc gggatagcac gcagaacagc ctcacgcaga gtaccgccct gtttcagctc
     1621 ccagttcacc agatgggcaa tcacttcggt gtcggtttca gaaacgaagg tatagccacg
     1681 cgcttttaac tcttcgcgca gcggttcatg gttttcgatg atgccgttat gcaccaccac
     1741 aatgtgttca gaaacatgcg gatgcgcatt cacttctgaa ggttcaccgt gggtcgccca
     1801 gcgagtgtga gcaataccgg tgccgccatg cagaggatgt tcttccgctg cctgagccag
     1861 catctggact ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat caacaacggc
     1921 cagaccggca gagtcatatc cgcggtattc cagacgacgt aaaccttcaa gaaggatttc
     1981 tgctacatca cgttgcgcga tcgcgccaac aattccacac atagtttttg attccgattt
     2041 atatcgttgt cggtcaacct gtatgcccgt tttttcgggc gccccgagcc ttgtagagag
     2101 tggggttatt tttatagtta ctgcttgtgg gagggagatt atgttatctc ctcatcccat
     2161 gtgaccggat tacccggcca gaatcacttt ttctttaccg gacgacgcca gccttctttc
     2221 tgagtctgcg gcacacggct gatagctaat gcattttcgc cgacattacg cgtcacagtt
     2281 gtacccgcag caatggtcgc gcctttgcct actgttaccg gggccaccag ctgagtgtcg
     2341 gaaccgacaa acacatcgtc gccgataatg gtcttaaatt tattcgcacc atcgtagttg
     2401 caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc gccaaggtaa
     2461 gtcagatgac cagctttcga gcctttaccc agacgtgctt ttttcatctc aacaaagtta
     2521 ccgacgtgtg caccttccaa caactcagca ccaggacgca aacgggcaaa cgggccaatg
     2581 gtacaggccg ccgccagatt cgcgtcttcc acgacggtat atgggctaat ttcgcaatca
     2641 tcgccaatca cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg atggccgaga
     2701 gtcacgttgc cctcgatgat aacgttagta tcaatttcaa catcgcgccc gtgagtaagc
     2761 gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa cagcagtttt
     2821 tcagcctgtt cggactggta aacgcgctcc agacgggaga gttgcaggcg gttattcacg
     2881 ccttctactt cgcttaaacg ttgcggatga acggcgacga tttcacgccc ttcctgatac
     2941 gccagcgcaa taatgtcggt gatgtagtat tcgccctgcg cattattgtt ggtcagcttc
     3001 gccagccagc gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga
     3061 atctgacgct gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac tttgccgttt
     3121 tcacgggtga tacgtccata accggtcgga tcatccagtt tcaccgtcag cagaccaatg
     3181 ccaccctgcg gtttagcatc acgcagacgc tggagtgttt cgacagagat cagcggcacg
     3241 tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatg
     3301 gcatgacccg tacccagctg ctctgcctga agcacccagt tcaggttgtc gtctttcagc
     3361 gcctgtttta gcagatcgcc accgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat
     3421 tcattcgcag catcaatgac atgctgaacc atcgctttcc cggcaagggt atgcagcact
     3481 ttcggaagat cggaatacat gcgcgtgcct ttacctgcgg caaggatcac tacgctcata
     3541 gcattattca acatacgcgt cctgactgta atttgagaac gaatttaaac cgcttcacct
     3601 tgaaaaaact acattttttt catcgtgaaa tggacagagg ataaattgtt caataacgga
     3661 ttatccccgc tgacgaaagc gccattttcg accatcgtca cgccttttgt ctgcagaaaa
     3721 ttaaggcagt accttcattt aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg
     3781 aataaacggg aaaacagggg gttttatatc agcaggatct atgtgaacgc tattcaggac
     3841 gggtcacacg cgcaaaaaaa agccagcctg tttccagact ggcttttgtg cttttcaagc
     3901 cggtgttaca tcgctttttt ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc
     3961 agttccgcag acgcctgagc gtaatctacg tcgccgtgag agctgctaat gtgctcttca
     4021 gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg
     4081 tcggccagaa cggtcacgtt gccaggctgc acttcaagaa tgccgccaga cagatagata
     4141 aactcttcgt gaccgtgctg tttcacgatg cgaatcatac caggcttaat ggcggtgagc
     4201 agcggtgcgt ggccagggta gatccccagt tcaccttcgc tacccgttac ctggattttc
     4261 tcgaccagac cagagaacat ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt
     4321 gccatatcac cctccgatta aggcgttaaa gttttttggc tttttccaca gcttcttcga
     4381 tggaaccgac catgtagaac gcctgctccg gcaggtgatc gtattcgcct tccatgatgc
     4441 ctttaaagcc acggatggtg tctttcaggg agacgtattt acccggagaa ccggtgaata
     4501 cttctgccac gaagaacggc tgggacagga agcgctggat cttacgagca cgcgctacca
     4561 ccagtttgtc ttcttcagac agttcatcca tacccaggat ggcgatgatg tctttcagtt
     4621 cctgataacg ttgcaggatg gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa
     4681 ccaccagcgg gtccagctga cggctggtgg agtccagcgg gtcaacggcc gggtagatac
     4741 ccagagacgc gatctgacgg ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg
     4801 ccggagacgg gtcagtcaag tcatccgcag gtacgtatac tgcctgtacg gaggtgatag
     4861 aaccggtttt ggtggaggtg atacgttcct gcagaacgcc catctcttcc gccagggtcg
     4921 gctgataacc taccgctgaa ggcatacggc ccagcagtgc ggatacttcc gtaccggcca
     4981 gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt
     5041 tctcagccat ggtcagaccg gtcagagcta cgcgcagacg gtttcccggc ggctcgttca
     5101 tctggccata caccagggat actttatcga taacgttgga gtcggtcatt tcgtggtaga
     5161 agtcgttccc ctcacgagta cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct
     5221 cgatcgcgat gttacgaatg agctccatca tgtttacggt tttacctaca cccgcaccac
     5281 cgaacagacc aactttaccg cccttagcga acggacacat caggtcgata actttgatac
     5341 cggtttccag cagttcctga gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa
     5401 tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc gaccggttca cccagtacgt
     5461 tcatgatacg gcccagagtc gctttaccta ccgggacttc gatcgggtgt tcgaggtctt
     5521 ttacatccag accgcgacgc agaccgtcgg aggaacccat tgcgatggta cgcacgatac
     5581 cgccgccgag ctgctgctga acttccagca ccagacgctc attaccattt tgcacctcaa
     5641 gagcatcgta cacgcgcggt acggcatcct gagggaattc gacgtcaact acggcgccga
     5701 ttacctggac aatctttcca gtagccatct taaatcctct acgaaataac ctgtttaaac
     5761 cgcggcggcc cccgagacga tctcggtgag ttcctgagta atgctggcct gacgagcttt
     5821 gttgtatacc aactgcagct ctttaatcag gctgccgcca ttgtcggtcg cggctttcat
     5881 cgccaccata cgggcggcct gctcgctggc caggttttca accacgccct gataaacctg
     5941 agattcgaca taacgacgca gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag
     6001 gtaatcccag gatttatgtt tcagatcatc atcatctgat gccggtaacg gcagcagctg
     6061 gctgatggtc ggaacctgag acatggtgtt aataaatttg ttgctgacaa tgtaaagctt
     6121 gtccagacgg ccttcgtcgt aggcctgcaa catcactttt accggaccga tcagttcgga
     6181 cagggaaggg ttatccccca tgccggtgac ctgggcaaca acattgccgc ccacggagtt
     6241 gaagaacgac acgcctttcg agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt
     6301 ccaggtcttc atttccgcca gcagtttttt gaacaggtta atgttcaaac caccgcacaa
     6361 accacggtcg gtcgacacca ccaggtagcc cacgcgttta acgtcgcggt cttccaggta
     6421 agggtgctta tattccagat taccgtgtgc aaggtgacca atcactttgc gcatggtttc
     6481 tgcataagga cggctggccg ccatgcgatc ctgcgattta cgcattttgg aagcggcgac
     6541 catctccatc gctttagtga tcttttgcgt gttctggacg cttgcgatct tactacgtat
     6601 ctcttttgcg ccggccatga gcttctcctc aatgccttgc ggcctgccct aaggcaagcc
     6661 gccagacgtt accaggattg ggttgctttg aaggaatcga ggatgccttt cagcttgcct
     6721 tcgatttcgt cgttgtagcc accggtctgg ttgatctctt gcatcaacgg agcgtgatca
     6781 cggtcgacgt aagccagcag agcggcttcg aagctgccga ttttcgacag ttcaacatcc
     6841 gccaggtaac cacgttctgc tgcgaacaga accagagact gctgcgcaac ggacatcggc
     6901 gcatactgtt tctgtttcag cagttcggtc actttttgac cgtggtcaag ctgcttacgt
     6961 gttgcatcgt caaggtcgga tgcaaactga gagaacgctg ccagttcacg atactgtgcc
     7021 agagcggtac ggataccacc ggacagtttt ttcatgatct tggtctgtgc tgcaccacca
     7081 acacgggata cggaaatacc cgggttaacc gcaggacgaa taccggcgtt gaacaggttg
     7141 gtttccagga agatctgacc atcggtaatg gagattacgt tggtcggaac gaacgcagaa
     7201 acgtcacccg cttgagtttc gataatcggc agcgcggtca gtgaaccggt tttccctttc
     7261 acttcacctt tggtgaaagc ttcaacgtat tcggcgttaa cacgcgcagc acgctccagc
     7321 agacgagagt ggaggtagaa tacgtcgccc gggaatgctt cacgtcctgg cggacgacgg
     7381 agcagcagcg agatctgacg gtaagcaaca gcctgtttag acagatcatc gtaaatgatc
     7441 agcgcatctt caccgcggtc acggaagtat tcgcccattg cgcaaccggc atacggtgcc
     7501 aggtattgca gtgcagcgga ttcagacgcg gttgctacca caacgatggt gttagccagt
     7561 gcgccgtgct cttccagttt acgtaccacg ttagaaatgg tggacgcttt ctggccgata
     7621 gcgacataga tacatttgat accggaatcg cgctggttga tgatggcatc gatagccagt
     7681 gcggttttac cggtctgacg gtcaccgatg atcaattcac gctgaccacg accgattggg
     7741 atcatggagt caacggcttt ataaccggtc tgtaccggct gatctacgga ctgacgttcg
     7801 ataacgcccg gagcgattgc ttctacagca gagaagccgt cgtgatccag cggaccttta
     7861 ccgtcgattg gtgcacccag agtgttaacc acacggccca gcaggccacg gccaaccgga
     7921 acttccagga tacggccagt acacttaact ttcatgcctt cggcaaggtc agcatacgga
     7981 cccataacaa ccgcaccaac agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta
     8041 cccggcaggg agatcatttc accctgcata caatcggcca ggccgtgaat gcggataaca
     8101 ccgtcactta cagaaacaat agtaccttcg ttgtgagctt cactcacaac attgaactga
     8161 gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat tcagttgcat gctccagtcc
     8221 ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg
     8281 accatatcac ccgctcggat gataacgcct gccattacag acttatcgat tttgcaattc
     8341 agcttaactt tgcgtgacag acgtttttcc atcgcagcag aaattttcgc gagctgttgt
     8401 tcactcagtg cggcagcgga aatgacgtct acctcagcgg tagcctcact cacggcacga
     8461 aggtgaataa actgctccaa aacatccggg agcgcgttaa gacgaccatt ttcagccatt
     8521 acccgaataa ggttctgacc gttttcgtcc agttgctcac cacaaactgc gataaacgac
     8581 tcggcaagcg tttctggcgc aagcgcgcca gagagaagct ctgccatttg ttcgtttttg
     8641 gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg
     8701 gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg taataaattc agacatcagc
     8761 ccctccctcc ttacagttca gcgacaagtt tatccacgat gtcgctgtta gcagcttcat
     8821 ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc caggatagca acttgcttac
     8881 gcagctcttc acgggcacgt ttacgctcgg cttcaatttc cgcctgcgcc tgggccacga
     8941 ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc cagaatctgc gagcggcgtt
     9001 tgttcgcctg ctcgatgatt acctgggctt ccgctttcgc ttttttcagc tggtcggtcg
     9061 cgctggcctt tgcaaggtca aggtccttat gtgctcgttc tgcggaagca aggccgtcag
     9121 caatttcttt ttgacgtttt tcgatggctg ccattaatgg cggccatacg tacttcatgc
     9181 agaacagaac gaacaggaca aacgcgatgg cctggccgag gattgttgcg ttaagattca
     9241 cagcacaatg cctctattta gttaacgttc tgatattgct ctttaaataa aagcaacgct
     9301 tactacgcga cagcgaacat cacgtacaga cccagaccta cagcgatcat cgggatagca
     9361 tccaccagac ccataacgat aaagaactga gtacgcagca gaggaatcag atcaggttga
     9421 cgcgctgcgc cttccaggaa tttacccccg aggatgccga taccgatcgc agcaccgatt
     9481 gccgccagac ccatcatcac agcggcagcc atgtacagca gatccatatt caggttttcc
     9541 atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc
     9601 agacgccatc gacagataga cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat
     9661 gatcaggatg tggaaaatgg cccacggcac attcaggatc cactgtgacc accacggcaa
     9721 cagaccagca atcagaatga aaatcagctc accggcatac atgttaccga acagtcgcaa
     9781 accgagtgaa actggtttgg acagcaggct taccccttca aggattaagt tgacaggaat
     9841 gaacgcccag tgattgaacg gctgcagcgt caactctttc gtgaagccgc cgatgccttt
     9901 cattttgatg ctgtagaaca gaatcaggat aaatacgccc agtgccatag acagcgttac
     9961 gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga cccagtacat gttcagcaat
    10021 gtacggcagc aggtcgatag gcagtaaatc catcaggttc atcaggaata cccagacgaa
    10081 gatcgtcagg gccagcggag caatcagctt gcttttgcca tggtacatgt ctttcacgct
    10141 accattaaca aagccgatca ccagctcaat cgcggtctga aacttacctg gcacaccgct
    10201 ggtcgccttt ttggctacgc tacggaataa aaccaggaac aacagaccca gcaccaccga
    10261 gaagaacatg gagtcaatat tgattgtcca gaaggtggct ggggggtttt gtggatccac
    10321 cagcgagaat gtacgcaggt ccagctgaag gttattcagg tggtgtccta tgtaatcctg
    10381 cggcgtcata ttttctgaag ccatgatgcc ttttaccctt tgttgttaat tacagccggt
    10441 gccagtatct gaaccaccag caccaaaacc cacgtaacga tcagcggcaa gaataccgcc
    10501 tttaaaaccg ccaacgccac caccagtaac accaacatcg ccagaacttt gaaagcttca
    10561 ccaaatgcga atgtccaggc cacccggcct ttcgctggtg tatgcgcctg gtgacgccag
    10621 gcaaatatca taaacaaaac gttaggcaga aagactgcca ggcccccgct tattgcagag
    10681 acgccccaga aggggtcttt gaggctgaac agcaatccac ttgctatcac caccagtaac
    10741 tgaacgagca gaagcttccg agcaacgttt cgactcacga gcgacacaga catcacgttt
    10801 ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata aaactttctt taaggcttag
    10861 agtcaagcat caaaaagcgg tcaaattata cggtgcgccc ccgtgatttc aaacaataag
    10921 tagccaaaag gtgaataaat gtttaaatat ttttccagtg catacaattg cgacttttct
    10981 actaaccctg ttcgatcatg aaaaactgta aataacgcgt aaacactggt gataaagcgt
    11041 gcttcagatc acatattgcg catgttcgcg cacagcatat ttatttactt ggcaaatgat
    11101 gcctttgcaa gtttatgata tttcagtcta aaaacagata ctgttttaat aaatg
//
LOCUS       DAZFTN010000145        10849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFTN010000145 DAZFTN010000000
VERSION     DAZFTN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..815
                     /gene="adiY"
                     /locus_tag="ACHKV2_004337"
     CDS             54..815
                     /gene="adiY"
                     /locus_tag="ACHKV2_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO6942103.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            952..2289
                     /gene="adiC"
                     /locus_tag="ACHKV2_004338"
     CDS             952..2289
                     /gene="adiC"
                     /locus_tag="ACHKV2_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO6942104.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2393..4036
                     /gene="eptA"
                     /locus_tag="ACHKV2_004339"
     CDS             2393..4036
                     /gene="eptA"
                     /locus_tag="ACHKV2_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO6942105.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4033..4701
                     /gene="basR"
                     /locus_tag="ACHKV2_004340"
     CDS             4033..4701
                     /gene="basR"
                     /locus_tag="ACHKV2_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO6942106.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHKV2_004341"
     CDS             4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHKV2_004341"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO6942107.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHKV2_004342"
     CDS             complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHKV2_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO6942108.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHKV2_004343"
     CDS             complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHKV2_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO6942109.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7745..8623)
                     /gene="rdcB"
                     /locus_tag="ACHKV2_004344"
     CDS             complement(7745..8623)
                     /gene="rdcB"
                     /locus_tag="ACHKV2_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO6942110.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8620..10848)
                     /gene="crfC"
                     /locus_tag="ACHKV2_004345"
     CDS             complement(8620..10848)
                     /gene="crfC"
                     /locus_tag="ACHKV2_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO6942111.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2708 a         2850 c         2684 g         2607 t
ORIGIN      
        1 atcgctttgc tttagcgaga actggtcttt tattcgcact caggagtaca tgtatgagga
       61 tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtta
      121 atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata
      181 atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca
      241 tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta
      301 gcgctacgcc cattctcacc ctgccatgcc tgacaccaga cgtctttcgc gttgccgcgc
      361 aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat
      421 tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt
      481 tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact
      541 ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagc
      601 tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg
      661 taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca
      721 acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgaca ccgctgcatt
      781 atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata
      841 ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta
      901 tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg
      961 gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt
     1021 atggggtcag gtgtttttct gttacctgca aacctggcct ccaccggcgg gattgccatt
     1081 tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg
     1141 tcgttcctcg acccaagtcc tggtggttct tacgcttacg cccgccgctg ctttggcccg
     1201 tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgcc
     1261 atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg
     1321 gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt
     1381 ccgaaaatga tcacccgtgt gcaggccgtt gccaccgtgc tggcgctgat ccccatcgtc
     1441 gggattgccg tatttggctg gttctggttt cgtggtgaaa cctatatggc ggcatggaac
     1501 gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac gctgtggtcg
     1561 ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaac
     1621 gtccctatag ccaccattgg tggggtattg attgccgccg tttgctatgt gctctccacc
     1681 accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcctc gccattcggt
     1741 gatgccgcac ggatggcgtt gggtgacacc gccggggcca tcgtttcctt ctgcgcagct
     1801 gcgggttgct taggttcact gggcggctgg acattgctgg cgggtcaaac ggcgaaagcg
     1861 gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc gggtacgcca
     1921 gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag cagcatttca
     1981 ccaaacgcga ccaaagaatt cggtctggtt tcttccgtgt cggtcatctt tacactggtg
     2041 ccatatcttt acacctgtgc ggcgttactg ctgctcggac acggtcactt tggtaaagca
     2101 cgcccggcat atctggcggt tactaccatt gccttcctct actgcatctg ggccgtggtg
     2161 gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat caccgccatg
     2221 tatgcgctga attacaaccg gctgcataaa aacccgtatc ccttagatgc accaataagc
     2281 aaagattaat tccccttaat ccagcaaaga taaaagccaa ccttaagaac ttaaggttgg
     2341 cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa
     2401 gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta
     2461 tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga
     2521 ctcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtagccttca gcgtgattaa
     2581 tattgtcctg acactaagct ctttcttatg gcttaatcga ccgctggcct gcctgtttat
     2641 tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc
     2701 gatgattgcc aatattattg ataccactcc ggcagaaagc tatgcgctga tgacaccgca
     2761 aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct gctggattaa
     2821 aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt
     2881 ttctgtactg ctgattttgc tggtcgccgc actattttat aaagactacg cctcgttgtt
     2941 ccgcaacaac aaagagctgg tgaagtcctt aagcccctct aacagcattg ttgccagctg
     3001 gtcatggtac tcccatcagc gactggcaaa tctgccgctg gtgcgaattg gtgaagacgc
     3061 gcaccgcaac ccattaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg
     3121 cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa ctaacccgcg
     3181 gttggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc
     3241 agtttcagta ccgtgcatgt tctcggatat gccgcgagag cactacaaag aagagctggc
     3301 acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa
     3361 tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc
     3421 gctgaatctg cctgatcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg
     3481 gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg
     3541 cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac
     3601 ctgcgacacc aatgagatcc agacctgtac caaagagcaa ctggtgaaca cttacgacaa
     3661 cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga aagaacatca
     3721 ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa
     3781 tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc
     3841 gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc agaactgcct
     3901 gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg
     3961 attaactggc gttgagacga agtattacca ggctgcggat gatattctgc aaacttgcag
     4021 gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggactg
     4081 attctggcgg cgcaaaccga aggctacgcg tgcgatggcg tgacaaccgc gcggatggcg
     4141 gaacaaagcc ttgaggcagg tcattacagc ctggtggtac tggatttagg gttacccgac
     4201 gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatataccct gccggtactg
     4261 atcctcaccg ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt cggtgccgac
     4321 gactatctgg tgaagccttt tgcgctggaa gagttacatg cccgtatccg cgccctgcta
     4381 cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaacatg
     4441 ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct
     4501 ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat tctctacaac
     4561 gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat
     4621 ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctt
     4681 gtcgcgaatg aggaaaacta attgaatctg atgcgttttc tgcgccgacc aatatcgctg
     4741 cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc
     4801 ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac
     4861 aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt
     4921 gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc ggtacgccgc
     4981 atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc cgacaactta
     5041 acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttgaac
     5101 gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttatttac cgctgacgtc
     5161 gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa
     5221 acgcatcaca ttgatgtagc accgttagtg gcacggcttg atcagatgat ggagagcgtc
     5281 tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat
     5341 gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac
     5401 cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat
     5461 gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagcccg
     5521 caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat ggccgttgaa
     5581 gatgaaggac cgggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt
     5641 atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag
     5701 ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg
     5761 gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct gctgaccacc
     5821 agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc
     5881 gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc
     5941 ctgataagac agcgttacat caggccatcc gtttcagctt attcatcaat tcgcggatgt
     6001 tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatatcatcg
     6061 attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct
     6121 gacgccgccg gtgttgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg
     6181 gtgattaaac caaccaccgc cactaccatc aggtaatagg ctggcatcat cagattctgc
     6241 gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata
     6301 ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta
     6361 gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa
     6421 atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagt
     6481 gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc
     6541 accggctgga caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct
     6601 tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa
     6661 gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca gtatttagtg
     6721 gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc
     6781 agtttatcaa catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc
     6841 ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag
     6901 ttcgcttcgc cgacaatggt cgaaattaac accaccacgc ccgcacccag cacaaacccg
     6961 gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc aggggagtat
     7021 tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc ttgtgccatc
     7081 ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag
     7141 ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga
     7201 ccatatttat cgcccaacat accaaagaag agtccgccaa gcggtcgaat cagaaaggga
     7261 acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa
     7321 actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca ttccattgca
     7381 ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca
     7441 tcacgaaggg taatcggttt tacttttttc cttttcagca tagctttcct cgcagagttg
     7501 gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt
     7561 gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat
     7621 gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagc
     7681 gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga
     7741 ttatttacac caaatttaca gagaagattt cctcgagcat tgcccccgcc agacgctgcg
     7801 ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc actgaccagc
     7861 aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa tcagcatgat
     7921 ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtggtcg
     7981 gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa tcacccagca
     8041 acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatgcc ccccaacgca
     8101 actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac
     8161 gccagcgggt aaaaatttgc tcgctctgtt cacccgcctg caccagtagg cctcgttgcg
     8221 ttaacgcctt ttccttccag cgacaacggg ctgaaaacca ctcatacaag gcggcaattt
     8281 gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca
     8341 gatgctgcgc gatctgcgtc agtgccagat gaccgggatc cagcatcgac gccagttttt
     8401 ccaccaatgt cagacgcaga ctaagcggcg catttaacat cccgccgttc gtccatgtct
     8461 gaagctgcac gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg
     8521 cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg ggataaaccg
     8581 actccagcac gcgaccgggg ccgtccagta acgtcttggt caatatcgtt ctgccgtgaa
     8641 aagtgtttga atatcatcgc gtaacagctg agcgtcggtg taaatccagc cgtgagtcat
     8701 cacagtctgc tgcaattgct gttgcatcag cctgaccacc gattcatttt gttgacgcag
     8761 agccagactt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga actcagcgaa
     8821 aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat
     8881 ttgttggcaa taaagatcga cttctgcgct taatgctcgt tgcaacacac tgtaatcaac
     8941 cgtttctgtc ggggatttct catttccccg tccccagtca ggctgattca accagcgcga
     9001 aaaagtctca cgcacaacgc ctaatcgcgt gctctgctcg tccgttgctt cctggcgcga
     9061 aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actacgccgt gaaaaacagg
     9121 aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat
     9181 ggggcgaata acatcggtta acacccggca aagggtggat tcaagctcgg caaaacgcag
     9241 cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcgggtacg
     9301 aatctcgctt aacggctccg aatcatcctg aaacaaggcg gctaactgca ccttcagcgc
     9361 atcttgctgt tgacgcagaa agtggttggc ggaggtcagg gccagctcga tttcatgttt
     9421 aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgatctg
     9481 gtggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag
     9541 gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcggcagatc gcagagcata
     9601 caacgaggcg ttagcgtaag cggcatgaag caacatctga attggctggg cgaacagcga
     9661 atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg
     9721 acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg
     9781 cggtggtaac ttaccgttgt tggctaactc atggcgcgcc cgatttgcca ggtagcccca
     9841 catcgacgac accggaaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga
     9901 aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt
     9961 gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc
    10021 atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc
    10081 cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt
    10141 atccagtaac gtcaattgac cgggataact ttccagcccc gccagatgga caaactccac
    10201 ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag
    10261 cgccttcgcc agtcgcacca aatcattcaa acttttcaga caatgaaaaa taggctgggc
    10321 acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat
    10381 atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg
    10441 ttgtaatttt tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca gtaccggttc
    10501 cttttggccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattgcgatt
    10561 aggcagtacc tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatggt
    10621 accgacaatc gccagcacca tttccagtcg ggaaatttta cgcaactcat tattcagcat
    10681 cgcgtgacgc tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcatcgtc
    10741 tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa
    10801 cagccgttca gcctcctgac ttaactcata cagggtctgt gtgtacata
//
LOCUS       DAZFTN010000146        10657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFTN010000146 DAZFTN010000000
VERSION     DAZFTN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..153
                     /locus_tag="ACHKV2_004346"
                     /pseudo
     CDS             19..153
                     /locus_tag="ACHKV2_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(258..328)
                     /locus_tag="ACHKV2_004347"
     tRNA            complement(258..328)
                     /locus_tag="ACHKV2_004347"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Met,seq:cat)
     gene            complement(480..1022)
                     /locus_tag="ACHKV2_004348"
     CDS             complement(480..1022)
                     /locus_tag="ACHKV2_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6942112.1"
                     /translation="MIYPTNTGKSGEHLRLNTLESIWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLTSKKLTKTAINEALRRLKKAGIDKPELEAFLREMIEGKQKSWLTHCTDAEAL
                     KIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWCFSTCEKRIANWL
                     AVAEYVLYIPMRESFAQKIA"
     gene            complement(1019..1309)
                     /locus_tag="ACHKV2_004349"
     CDS             complement(1019..1309)
                     /locus_tag="ACHKV2_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6942113.1"
                     /translation="MVDLRKAAKGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWREKGIL"
     gene            complement(1309..1908)
                     /locus_tag="ACHKV2_004350"
     CDS             complement(1309..1908)
                     /locus_tag="ACHKV2_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO6942114.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPNGGAITPRERELVSGFVDFLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1980..2192)
                     /locus_tag="ACHKV2_004351"
     CDS             complement(1980..2192)
                     /locus_tag="ACHKV2_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942115.1"
                     /translation="MEKIKKLFSCKYAVIRRDDLSVIVEMDYFPETPKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
     gene            complement(2468..2623)
                     /gene="hokD"
                     /locus_tag="ACHKV2_004352"
     CDS             complement(2468..2623)
                     /gene="hokD"
                     /locus_tag="ACHKV2_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6942116.1"
                     /translation="MKQQKAMLVALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2803..3924)
                     /locus_tag="ACHKV2_004353"
     CDS             complement(2803..3924)
                     /locus_tag="ACHKV2_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942117.1"
                     /translation="MELFDSIGNYRGDCLKLARQAKGVTGEELATALGVTRQFINKLE
                     RGYPPSSQMLTALADYLDVKESFFFTGRKSPLDSENCHFRSKRSRTQSLTNSILARAE
                     ILEQFVKCLETEIVLPELNFPDTSGLPLNSDEDIERIAEACRRYWGLGLGPISSMVNF
                     VESIGVVVAHVSGVDDRIDAFTINSKRPLIIRNSAKKSVCRFRSDLGHELGHLVLHEG
                     IITGDKITEHHADYFSSAFLVPRVSFVNEFPKMRGSHLDWNALINFKERWKISLRMCI
                     YRATVLGLITPQQMRTGFIHLNKRGTIKGEMGDELIPEEKPRLLSCAVELLDISSWKQ
                     ILDMSGVRERFVSKMFGIRRTHDDISSNIVPLYRYKDFG"
     gene            complement(3911..4561)
                     /locus_tag="ACHKV2_004354"
     CDS             complement(3911..4561)
                     /locus_tag="ACHKV2_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942118.1"
                     /translation="MPSEQNLQPCFEINPCLDEKSVINLLQQFANVLGELWFHRHEYD
                     DNWINGCRAYGWIKNFLVKNHKKLKGVAILSTPLNFVFTLNRVPLQFVTDKLDSPKKK
                     HRLCKNDVECRQLSLFEDDVNFEHDVTWRVLAEKLPSANKKDEDIEPPTWLLALIGFD
                     SSNNVVSRYELVSKVHAPVQAANTDVLPEAVETPPLNLQRRVKNAKKADSDKYGTI"
     gene            complement(4716..4910)
                     /locus_tag="ACHKV2_004355"
                     /pseudo
     CDS             complement(4716..4910)
                     /locus_tag="ACHKV2_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4943..5365)
                     /locus_tag="ACHKV2_004356"
     CDS             complement(4943..5365)
                     /locus_tag="ACHKV2_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6942119.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRQLEEKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRANLKKTSVDDPDLIWSLPDG
                     EIRRYDRRQNIICSECRKSEVMQRALAFYQGNFQEVAQ"
     gene            complement(5381..6142)
                     /locus_tag="ACHKV2_004357"
     CDS             complement(5381..6142)
                     /locus_tag="ACHKV2_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6942120.1"
                     /translation="METVFGALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPMQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PACVALPVQEAATQEDIKTETVADIVQSLPSFTATRSDDLILSSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDFSRRVASEK"
     gene            complement(6165..6911)
                     /gene="ydaV"
                     /locus_tag="ACHKV2_004358"
     CDS             complement(6165..6911)
                     /gene="ydaV"
                     /locus_tag="ACHKV2_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6942121.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(6918..7706)
                     /locus_tag="ACHKV2_004359"
     CDS             complement(6918..7706)
                     /locus_tag="ACHKV2_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942122.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRISFMAGFADALIQVGWLVENDVGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAAGVTQTDQKALPEEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEL
                     VIRNSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(7784..8206)
                     /gene="ydaT"
                     /locus_tag="ACHKV2_004360"
     CDS             complement(7784..8206)
                     /gene="ydaT"
                     /locus_tag="ACHKV2_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6942123.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITDEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            complement(8203..8445)
                     /locus_tag="ACHKV2_004361"
     CDS             complement(8203..8445)
                     /locus_tag="ACHKV2_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942124.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            8542..8961
                     /locus_tag="ACHKV2_004362"
     CDS             8542..8961
                     /locus_tag="ACHKV2_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942125.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRLKDNPPPE"
     gene            9268..9420
                     /locus_tag="ACHKV2_004363"
     CDS             9268..9420
                     /locus_tag="ACHKV2_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6942126.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKSFKTEAGARRW
                     LAKNTS"
     gene            9832..>10657
                     /locus_tag="ACHKV2_004364"
     CDS             9832..>10657
                     /locus_tag="ACHKV2_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6942127.1"
                     /translation="MLDVAIENQNGWNYSAPAPHKTGAGICTPMITRAHNRAKAVFLC
                     VKRSHIQIMVGRMGPFSGGPDSLVTGSANPVRLTTHEICTSGGEFTNLSLEAAIMATI
                     PTLSHPDVTIENGRAVTTSVAVAEFFRKLHKNVIQKIEALECSSEFTELNFKPSEYTD
                     STGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTTPTNKII
                     PGDGRTLVVRFDKFGNVEFTETVPDGALVCTLDTFRLYLEKQGWTLVNRSAIKNMTV"
BASE COUNT         2702 a         2749 c         2276 g         2930 t
ORIGIN      
        1 gcccctccgg agaggggcat gtttgcatgc acattctttt tcttgcatgg tgccgggtgc
       61 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
      121 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
      181 ccagacaaca gtcaaccgcc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
      241 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagcgacct ggcgattatg
      301 cgtcgctcgc tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat
      361 taattccgca ataaaaaacc cgctcaatgg cgggttctgg taaagttcat gcgcttggtt
      421 cgcctcgcga tacagctttg cgaagcgtac cggaattgaa gcagtttatg cgtaaaaaat
      481 caagctattt tttgagcaaa tgattctcgc atgggaatat ataggacata ctcagcaact
      541 gccaaccaat tagcaattcg cttttcgcat gtgctaaaac accactctgg gtactgttcg
      601 tttaataact cggccatcct tctcttactc atccctcgcc ccacataacg ctgactcagg
      661 acatttagta gtcctggatg atccgccaga acttcaccta taacactatc aatttttagt
      721 gcctctgcat cagtgcagtg agttaaccag cttttttgct ttccttcgat catctcacgc
      781 aaaaatgctt ccaactctgg tttatcaatt cccgcttttt tcaatctgcg cagggcttca
      841 ttgatggctg tttttgtcaa ttttttggat gtcagcaact gattaaacat attccctgac
      901 ctgccgccac caatgtacga ccagcgcccc cacatacgca gtttcccctg tatccagatg
      961 ctttccaacg tattgagtcg aagatgttct ccgctttttc cggtatttgt tgggtaaatc
     1021 atagaatgcc tttctccctc cagatttcct gtgtgcggaa aacgccctcc gcgtgcatca
     1081 ggcgcaattc ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc
     1141 acgagctaca ggcaattgcc gcctgcatat cgtgtggttt tgtcgctgtt ccacacgtcc
     1201 ccgccagcct gtaatgcgcc agcacggacg tttcgggatt gtgattgcag tagccaggaa
     1261 ttctgactgt acacatctga ccttttgccg ctttacgtaa atccaccatt acgcaaactc
     1321 cagtagctgc gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacaggat
     1381 ccagttccac agtacattca gcacagattt ataaacctgc tgaaactcgg tttcgtccat
     1441 attcgcaaac gcgatggatt tcgccctgcg cccacggcta ccgtccggat aaaaatgctc
     1501 ggtgtaaaat ccggcctgaa tggttaccca ctcgcggaaa gcctcaaacg actttagcaa
     1561 tgccgtatcc cgggttctgc gtgtcgcaac ggtattcaga tattgctctg cggcatcact
     1621 cagggctgga gtatgttccc gacctactga ttcgcacagg aaatcaacga aaccggacac
     1681 cagttctcgt tcgcgaggcg tgatcgcccc accgttcgga gtccagtaat cgaatcccag
     1741 ttgcaggagt ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc
     1801 gtgtatccac tcaccgattt tgatttgatg caaaaaatcg caactctccg gcgtcgccgg
     1861 gagaagtaat ccggaagagg tttgtttgac cagttgtata tgcgccatcg tagttctccg
     1921 ctggcgcagt agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac
     1981 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
     2041 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttggggtt
     2101 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
     2161 ttgcaactaa atagtttctt tattttttcc acgatgcctc cgagtttata agtacaaacg
     2221 gttatatcca catagagaca aaaatattaa tctgaaaaat atttatttca cgccgtatat
     2281 ttgattattt aatgtgcagg tacaatgact tttatttttt gttgtgtata taatcaaata
     2341 tatggttatt tttcaccctg cgcattcagc gcgcaacaaa aaacccgccg aagcgggtta
     2401 agtgcgggtg cgttgaggat gcctgacaca tcagaggtgg cgagggattt ctcccccgcc
     2461 aggtctctta ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc tggccggttc
     2521 ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc cgtcactatg acggttaaac
     2581 agatgacgat cagggcgact aacatcgcct tttgctgctt catagcctgc ttctccttgc
     2641 ctttcggcac gtaagaggct aacctacgtt tgtgaagcat agattgggcc tcagattaat
     2701 gttaagcgtc ttgcaggacg cgtaatgtta actggggctt ttctctatct gccttttggt
     2761 gttcatgcct gaggcagata gcctcaagca cccattatga ttttaaccaa agtctttgta
     2821 tctataaaga ggcacaatat tactcgatat atcatcatgt gtgcgcctta ttccaaacat
     2881 tttggaaaca aagcgttccc tcactcccga catatccagt atctgcttcc aagaagatat
     2941 atccagcaat tcaactgcac aggaaagtaa gcgaggtttt tcctcaggga ttaactcatc
     3001 ccccatctct cctttaatcg tccctctttt atttagatgg ataaatcccg ttctcatttg
     3061 ttgtggcgta atcagcccca aaaccgtagc acgataaata cacatgcgaa gactgatttt
     3121 ccatcgttct ttgaaattaa ttagtgcatt ccagtcaaga tgcgatcctc gcattttagg
     3181 aaattcatta acgaacgaaa ctctaggtac aaggaatgcg ctcgaaaaat aatccgcatg
     3241 atgttctgta attttatccc ctgttataat cccttcatgc aaaacaagat gccccagttc
     3301 atgaccgaga tctgagcgaa atcgacacac actctttttc gcggaatttc taattatcaa
     3361 tgggcgttta ctattaatag taaacgcatc aatacgatca tccacgccag atacatgagc
     3421 aacaacaacc ccaatacttt ccacgaaatt aaccattgaa gatatcggtc ccagccccaa
     3481 accccaatac cttctacagg cctcggcaat tctttcaata tcttcatcgg agttcaaagg
     3541 taatccggac gtatcaggaa agttcaattc tggaagaact atttctgttt ccaaacactt
     3601 tacaaactgc tccaaaattt cagctcgagc cagaatacta ttagttaagg actgcgtcct
     3661 tgatctcttg cttctgaaat ggcagttctc gctgtctaat ggtgatttcc ttccagtgaa
     3721 gaaaaacgac tctttaacat ccagataatc tgcaagagct gtcaacatct gagatgaagg
     3781 gggataacca cgctctaact tgtttataaa ttgccgagtc acccccaaag cagtagcaag
     3841 ctcttcgccc gtaacccctt tagcctgacg agctaatttt aagcagtcac cacgataatt
     3901 acctatagaa tcaaatagtt ccatatttat cactatctgc ttttttcgca ttcttaacac
     3961 gccgctgcaa attcagcggt ggtgtttcca cggcctctgg caatacgtca gtattagctg
     4021 cttgtactgg agcatggact ttagacacca attcgtatct actcactaca ttatttgatg
     4081 aatcaaaacc tatcagggct aaaagccacg ttggaggttc aatatcttca tcttttttat
     4141 ttgcagatgg taatttttca gccagaacac gccatgtaac atcatgttca aaattaacat
     4201 catcttcaaa aagagataac tgacgacact caacatcatt tttacataag cgatgtttct
     4261 tttttggtga atccagttta tcggtcacaa actgtaacgg caccctgttc agggtaaaca
     4321 caaaattcag aggtgttgaa agaatcgcca ctcctttgag ttttttatga ttttttacaa
     4381 gaaagttttt tatccatcca taggccctac atccattaat ccagttatca tcatactcat
     4441 ggcgatggaa ccataattcg ccaagtacgt tagcaaattg ctgaagaaga ttgataacag
     4501 atttttcatc caaacaagga ttgatttcaa aacacggttg gagattctgt tcagacggca
     4561 taaggtatcc ctctctctta aaactccgct catctttacg caaaatttta tttttgtaaa
     4621 cctaatcttt aggggatttc acgaattcgt caaccagtcg aacaaacttt aaccacaata
     4681 catatttatc tacattgata tgtgtatcgc taacactatt gtgggcattc agctagtatt
     4741 accaagcctt caggtgcgcg aagttgttcc gcacagtgca gcagcgcatc agtcgcttcc
     4801 ttaagcgtca cagtgtcgcc atcacccagt cctgcaattt ttgcgtgcct gacaaacgcc
     4861 gcgcaaaggt cgttaaacgc ccctgcccgc acatccgcca ggaaagcgtt ggcagcagcc
     4921 aaatcctggt agtcaatccc gctcactgtg ccacctcctg aaaattaccc tgataaaacg
     4981 ccagtgcgcg ctgcataact tcgctcttcc ggcactcgct acagattatg ttctgacgcc
     5041 tgtcgtagcg gcgtatttct ccgtctggta atgaccagat aaggtccgga tcatccacag
     5101 atgttttctt cagatttgcc cttgagagtt ttttgcgggc gttttgccag tccttacgcg
     5161 cctgttcaga cgggaataac ccgtaaccag agttgtatac atcgccactg gcaaccagct
     5221 ctctggcgag aacactcatc agatatcttg tcgcacctgt tttctcttcc agttgccgta
     5281 acgtctcgcg gccgctcaga cgtacaagtt caacaacctg ccctttaatt ttttcccgct
     5341 cttctggtgt aaatactttt gccatgggcg cctccggcaa tcacttttcc gatgcaacac
     5401 ggcgggaaaa atcagtaatc tgtcgaacaa tatcccgatg cttgttcagc tcccgcagcg
     5461 cggcgcagac acgctcccac ttctggacat gatttttcgc ccgacgcagt tcacggtttg
     5521 ccatatgcag cgatgacaaa atcaaatcat cagatcgcgt tgcagtaaac gatggcagcg
     5581 actgcacaat gtccgccaca gtttctgttt taatatcttc ctgtgttgca gcctcctgta
     5641 ctggtaacgc aacacatgca ggctgaggaa aggctttacc atcagtttcc gctaccgatg
     5701 ctgctttcgg ctctgctggt aaattatcgc ccggtatgca gtaacgaaat ttaccgttct
     5761 ggtttacgcg aatcaggcgt cctttgctga ttgccattgc cagcgttgaa gcaactttgc
     5821 gggatgttgt accgaacagc gtagccagtt catccgccgt ttgtggtccg cgttgttcaa
     5881 tcgtcgcggt taaatcgcac tctgagattt tcgctactgt tgccgtggtg gtttcttccg
     5941 gcagttctgc ctgcattggc tgttcctgct gaacgttgtt atcggccaca cgccaggtgt
     6001 atacgctttt atcaacgaat ccagcctttt tcagttccca aagctcgttc agcacttctt
     6061 cacgactgat atcaagtcgc gcagccagtt ctaccgacgt ggcttttccc attgctttca
     6121 gtgcaccaaa aacggtttcc attaaaattt cctcccggta aaaatcactt cgcaattcct
     6181 ggctggacga cattcggacg ccagctctcc cagttaaaat tcacccatcg cccgccgttc
     6241 atggtcatgc gatccataat cctctcgccg agcaatgttt tcatggcctc atagttcagg
     6301 tttgtcagca tccccacgct gcgcatcgac gctgtccggc gatcaacaat ctggtgcagc
     6361 accacctgct cgttttttgt ctcgcgctga atgccaattt catcaagaac cagcagatcc
     6421 acttcgcaca gttcccgcaa aaatttttcg cctgactgcc cgtcgtcata gctggcgtgc
     6481 agggcgctca taacatcagc cacggtaacc acaatcactg tctgaccgtc tttcagcagg
     6541 cgattcccga tagctgccgc taagtgattc ttcccggtac caggttttcc gctgaacgca
     6601 aaatttgtac acccggtcat cagttcatcg gcgatggatt tcgcctggct taacgcgtat
     6661 cgctggccgt cgttctgcac ctggtaattc gcaaacgagc atttacggtg caacggctgg
     6721 atgcctgagc gattcagaat tttttccacc cgcaactgac gattcaggcg gttgatctcc
     6781 tcgctacgtt tctggccttc agcaagttgc cactcgcgcc actccgcaac cgttctgaat
     6841 ggggcggtta catgtggtgg ggccagtctg cggatacgct ccagaacgcc tcctgtcgca
     6901 atatttttca tggtctgtta ccccctgaag cctggcggga tcgcactgtc cggcaacgag
     6961 acggtgttaa cctgtcggag caacgtctca ggccgaacac ctttcggcgc gaacaggccc
     7021 tggtattcat tggcgatgct gtttcgaatc accagctcag gtgtaaaacc ctgctgacgg
     7081 aatttttcca gttcccgtat cgccccgtta gcgccctgct ccgttcgaat cggttttcgc
     7141 aatgcctgtc tgaaccggac ccactcatgc cagagtgttt ccggcaacca atcgggcagc
     7201 tcaatagcct ccggctcgaa ttttttagac gctcgttttt ggcgaggggg atttaggggg
     7261 agatcagtat ttagatcttc ctcttcctct tcctctggta acgctttttg atccgtttgt
     7321 gtaacgccgg cagcgttacc ttttcgtttc agttcacgta tttttgtaac tcgctcgttt
     7381 gtaaccgccc gttttttaga gctttttccg ttatggcgtt caaagttagg tagagaaagc
     7441 ccaacgtcat tttcgaccag ccatccaacc tgaattaacg catcagcaaa accagccata
     7501 aaagagatgc gatctattgc actttttgta acgccgcgag cgttacaatc tgcattaccg
     7561 tctatcattt gttgatccgc ccatgcccag aagcgaataa ccttccctaa tgcggcatct
     7621 ggatcaatat tcagaatctc agcaagcctg aatatttccg gcttatccgg cgtaataacc
     7681 tcgagcttta tccagtttga agccatttgt tttcaccttg taacgctcgc agcgttacat
     7741 ttaactgata ccgaacaaaa caatccggca cgattaattt caatcaatgc actacgacag
     7801 aatcaccggg cgacccacca ccgctgaaat gtgctttccg gtaaacggcc tggactgcat
     7861 catcatgcgc atcaattgcc gtactcaacg cttcctgcgc cgccagtaat gcacggcgtt
     7921 ccagggtatc gaagatgcag agtcggtgac gcagctcgcg cggaagaatt gccagaaccg
     7981 cagggatcag tttctgaatt ttttcccttt gcgctttcgt ttcacctttc aaccaacggt
     8041 gatagatatt ctgctgattg ttccagtcct tgcctggtac caggggcaat tcgccgcccc
     8101 cctggcgcag atattcatca gtaattgcgt tagcgaccca cgcctgccct ttttcggctg
     8161 ccagggctaa cagcactgat tcgatgtgct catgcctgat tttcatgaat caaccgctcc
     8221 tatgctgttt tcgctatgct taccgtctgg ggggaataca tcgtcaagtc cacaatgagc
     8281 gccaagccga ttaagggtag aaacaatttt tctgcactcc tctagtccag gggtacgaaa
     8341 atttgcttcg taatttgcca gtcggctttg tatccaccct aactgaacag caagttgtct
     8401 ttgagacagc ccaagctgtt ttcgatatgt tgaaattttg ttcattgaaa acctccgatg
     8461 acaattttaa acacatcttg tgttatatgg tcaagctgtt ttgtgttttg tgtaaatcac
     8521 gattcgtgat acaaggatgc aatggaaaaa gaaaacgaaa aaattgccgc tagtaggctc
     8581 aatgacaaaa ttgcaatgcg tcttaaagag cgcaggcaga agcttggttt atctcaagga
     8641 aaacttgctg aaatctgcgg atggacgcaa tcgcgtatag gtaactatga ggcgggtagc
     8701 agaaatgttg gagtgcatga cgctgtcgta ttgggaaagg cacttggcat atctcctcct
     8761 gagctcctct ttggagaaca ggaatcttct gaattgtggt taaatgaatc ccaacgaaaa
     8821 cttcttgagt tgtttaacca gctaccgggc tcagaacaac aacgaatgat tgagctattt
     8881 gaagtccggc taaaagaaat cgatgagtat gtagaaaaat atttgagagg caggcttaaa
     8941 gataatcccc caccggagta atgatcttgc tatcacagta atatgccaat cagcccgcta
     9001 tcagcgggct ttttttgtac catcatcata tgacacccac cacaaaacac atttcgtgtt
     9061 gacacaagaa aacgcattgt gtttaataag catatccaaa caacgcccca ccagagaacg
     9121 gcaggacaat acctcgagtt atccagccac tgaacagggc taagtagcca gcctgaggca
     9181 tacgaacatg acggcagttg ttgattgata caaagcgcag tagataaaac gttccgccac
     9241 ccggcgttaa ggggaaatga ggtcaacatg gatactatcg atcttggcaa caacgaatct
     9301 ctggtatgtg gcgtgtttcc caatcaggac ggcacattca ccgccatgac gtataccaaa
     9361 agcaaatcgt ttaaaaccga agctggcgca cgtcgctggc ttgccaaaaa taccagctaa
     9421 accatttatt ggattaattc aatattcttg ctgtaggggt atagccgagg ccaccaaagc
     9481 ccggaggtgg tgaaataaaa ccgggcacaa cacgaaggcg catttccgat atccataaag
     9541 agtcggtctt gtctgttaaa tttaaatggt gggagtgcgc ctccggttgt aaataacgac
     9601 attgctgtgt gtagtcttgg cggcatcagt tctactccgt ggctgccctg ccgccccttt
     9661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctgagcgc acgcggcaca gttaaaagca
     9721 tcagtgttat gggtggatta tccggcgtta attgttaact ggttaacgtc acctggaggc
     9781 accaggcacc gcatcaacaa agttcacttc agtgatgaaa ggtgagagaa aatgttggat
     9841 gtagctattg aaaaccagaa cgggtggaat tatagtgcac ctgcacctca taaaacgggt
     9901 gccgggattt gcaccccgat gatcactaga gcgcataacc gcgccaaagc ggttttttta
     9961 tgcgtaaagc gcagccacat tcagattatg gtggggcgta tggggccgtt ttcgggcggg
    10021 ccggattctc tagtgaccgg tagtgcaaac cctgtacgtc tcaccaccca tgagatttgc
    10081 acctccggtg gtgagtttac caacttatca ctagaggctg ccatcatggc tactatccct
    10141 accctttctc accctgacgt aaccatcgaa aatggtcgtg ctgtcactac gtctgttgca
    10201 gttgcagagt ttttccgcaa gctgcataaa aacgtcattc aaaaaattga agctctggaa
    10261 tgttcctcag aattcactga gcttaatttt aagccaagtg aatacaccga ctcaaccggg
    10321 cgcaaactcc caatgtacca aatcaccaaa aacggcttcg ttttcctggt gatgggtttc
    10381 actggcaaaa aagccgctgc attcaaagag gcctacatcg ctgagttcga ccgcatggaa
    10441 gcagagctac gccagaataa taccactccc acaaacaaaa tcattccggg cgatgggcgc
    10501 actctggttg ttcgcttcga caaattcggc aacgtcgaat tcactgaaac cgttcctgat
    10561 ggcgctctcg tctgtaccct ggacactttc cgcctttatc tggagaaaca gggctggact
    10621 cttgtaaacc gaagcgcaat taaaaatatg accgtcg
//
LOCUS       DAZFTN010000147        10146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFTN010000147 DAZFTN010000000
VERSION     DAZFTN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            177..329
                     /gene="hokA"
                     /locus_tag="ACHKV2_004365"
     CDS             177..329
                     /gene="hokA"
                     /locus_tag="ACHKV2_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO6942128.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(306..377)
                     /locus_tag="ACHKV2_004366"
     CDS             complement(306..377)
                     /locus_tag="ACHKV2_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942129.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(517..729)
                     /gene="cspA"
                     /locus_tag="ACHKV2_004367"
     CDS             complement(517..729)
                     /gene="cspA"
                     /locus_tag="ACHKV2_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO6942130.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1010..1300)
                     /gene="yiaG"
                     /locus_tag="ACHKV2_004368"
     CDS             complement(1010..1300)
                     /gene="yiaG"
                     /locus_tag="ACHKV2_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO6942131.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1734..2444
                     /gene="yiaF"
                     /locus_tag="ACHKV2_004369"
     CDS             1734..2444
                     /gene="yiaF"
                     /locus_tag="ACHKV2_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO6942132.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2494..3468)
                     /gene="ghrB"
                     /locus_tag="ACHKV2_004370"
     CDS             complement(2494..3468)
                     /gene="ghrB"
                     /locus_tag="ACHKV2_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO6942133.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3572..4231)
                     /gene="yiaD"
                     /locus_tag="ACHKV2_004371"
     CDS             complement(3572..4231)
                     /gene="yiaD"
                     /locus_tag="ACHKV2_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO6942134.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4384..6717
                     /gene="bisC"
                     /locus_tag="ACHKV2_004372"
     CDS             4384..6717
                     /gene="bisC"
                     /locus_tag="ACHKV2_004372"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO6942135.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6686..7126)
                     /gene="yiaC"
                     /locus_tag="ACHKV2_004373"
     CDS             complement(6686..7126)
                     /gene="yiaC"
                     /locus_tag="ACHKV2_004373"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6942136.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7123..7686)
                     /gene="tag"
                     /locus_tag="ACHKV2_004374"
     CDS             complement(7123..7686)
                     /gene="tag"
                     /locus_tag="ACHKV2_004374"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO6942137.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7844..8542
                     /gene="yhjY"
                     /locus_tag="ACHKV2_004375"
     CDS             7844..8542
                     /gene="yhjY"
                     /locus_tag="ACHKV2_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6942138.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8771..9973
                     /gene="yhjX"
                     /locus_tag="ACHKV2_004376"
     CDS             8771..9973
                     /gene="yhjX"
                     /locus_tag="ACHKV2_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6942139.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2429 a         2449 c         2596 g         2672 t
ORIGIN      
        1 acaaacttat taagctagaa tagccacggg tgcttgagac tgtttgtctc aggtattcac
       61 cgaaaggcag acagagaaaa gccccacctg actataaatc aaagtgaggc ccctctatgt
      121 atagacacca taaggttagc ctcttactcg ttggaaatca acacaggggg ctgggaatgc
      181 cgcagaaata tagattgctt tctttaatag tgatttgttt cacgctttta tttttcacct
      241 ggatgataag agattcactg tgtgaattgc atattaaaca ggggagttat gagctggcgg
      301 catttttagc ctgcaatttg aaagagtaag agtcttcggc ggaaagttat tcccgccttt
      361 cttacggcgt tgcgcattct cattgcaccc aaatttattt ttcacaaaaa taatactaga
      421 ttttattacg cgatcgatta tttatttcct gaaaaacaaa taaaaaaatc cccgccaaat
      481 ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta cgttgccagc
      541 tgccgggcct ttagcgccgc tttcgatggt gaaggacact ttctgacctt cgtccagaga
      601 tttgtaacca tcgttctgga tagcagagaa gtgtacgaac acgtctttag agccatcgtc
      661 aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac cagtcatttt
      721 accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata tggcgtgctt
      781 tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga taactcttga
      841 agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg atgtgcatta
      901 agccacgcat tggcgggtga tgcaacaatt atttttcata tttatgatta atcggttgga
      961 aaacggtcct gtcatcagga ccgtaaacag caataaagtg gataaaagtc tattccatca
     1021 actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt tcggcacttg
     1081 aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg cccaaaaccc
     1141 gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa ctgctcaatt tcggtacagg
     1201 acgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca atctgttcca
     1261 ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc tcaaatcaca
     1321 ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag cagacacagc
     1381 ggggtgaagt cagggcatgc tctggctatg attcgattca gccagtcatt tagcgtgact
     1441 tatagccacc taacgatctg ataaatgccg atatatctct aaccactcct ggtttgtaaa
     1501 gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa aaggcaacgg
     1561 caatatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc tccatgattt
     1621 taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta tccttgtcac
     1681 gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga
     1741 caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg gtagttagcc
     1801 tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaga
     1861 atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag aaaaaacagt
     1921 ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag gtgaatcagg
     1981 cgatggattc cggtctgcgc ccggttgtag acagcgttaa cgcaattcgc gtaccgcagg
     2041 attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt gtgctggcgc
     2101 aacaactgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta aaacagagtg
     2161 atgacctgaa accggtcttt gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg
     2221 cattgcaacc gttgattccg gcggcacaaa ctttcacgca acaactggtg atggttggag
     2281 actacattgc ccaacaaggt actcaggtga gctttgtagc aaatggcatc cagttcccga
     2341 cttcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca caacatcagg
     2401 cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta aaagcccgag
     2461 cgattatcgg gctttactcc acgcagtcgc ggcttagtcc gcgacgtgcg gattcacaca
     2521 gttcttctca acctttcctt gtaacgcatc aatcaaatta tccacggcac aggcggccat
     2581 gccataacgc gtctcatggg tggcagatcc aatatgcggt actgcgacga cgttggccat
     2641 tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg aagacatcca gcccggcggc
     2701 gtgaatttcc cctttctgca atgctgcgat cagtgcattt tcgtcaacca ccgggccacg
     2761 tccggcatta atgaaaatgg cggaggattt cattttggcg aattgttctg cgccaaacag
     2821 atgatgcgtc tcatcagtta acggcaggat caggcaaacg aaatctgact cttgtaacag
     2881 tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat ggtggcggcg
     2941 cgcgttatag aggatgggca tgttgaagcc aaagtgcgca cgttgtgcca gcgccatacc
     3001 gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc
     3061 cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct ccacaacccg
     3121 acgagcggta gacaacacca gcgccatcag cgtatcggcg acggtttctg ttaagacggt
     3181 tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa aattgtcata
     3241 gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca atgcggcatc
     3301 aacattctcg tttgaaccca gtaaaccttc agcttcggca aaaattgctg cattttgttc
     3361 gacggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgctctt gcaggcgttg
     3421 cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc ttctctccat
     3481 tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga agaatgccag
     3541 gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta atttctacac
     3601 ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta gccgggccaa
     3661 ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg
     3721 aatccgcacg ctgctgggag agacgcatgt tcagatcgtg accacccgtg ctgtcagtat
     3781 aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc acgccggtca
     3841 gggtgttagc gcccgccggt ttcagggttg cgctgctgct gtcgaaggtc acattgttcg
     3901 gcatattgag gataatgtta tccccgctgc gggttacgct aacaccagtg ccgcgcattt
     3961 tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg cccagagctg
     4021 cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg
     4081 caccaatacc cgcgcccacg agagagccca gacctgcgcc gatagcagat ttacctgctt
     4141 cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg ccactcacta
     4201 cggcggcaat aagataaaca cgtttcttca ttgttaatcc ttaataacct ttttattctt
     4261 tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat tcctgggaat
     4321 actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc aggagtcttc
     4381 ggtttgacca actcatcctc acgatattcc gttctgactg ccgcccattg ggggcccatg
     4441 ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc cacaggaatg
     4501 gaaaactcct tgcagagcgc ggttcgtgac caggttcaca gcaatacgcg ggtacgattt
     4561 ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat tcgtgggcag
     4621 gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca acaacataaa
     4681 cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg ctggcgttca
     4741 aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct ggcaggcggt
     4801 tataccgggc atctggggga ttattcgacc ggcgcggcac aggcgatcat gccgtatgtc
     4861 gtgggtggta gtgaagttta tcaacagcag accagttggc cgctggtgct ggaacatagc
     4921 gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc tgaaaattgc gtggaatgca
     4981 tccgatgagc aggggctttc ttacttttct gcactgcgtg acagcgggaa aaagctgatc
     5041 tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg
     5101 gcaccgcaca tgggcactga tgttgcgctg atgctgggga tcgcccatac gctggtggaa
     5161 aatggttggc acgacgaagc gtttctggcg cgttgcacca ccggttatgc cgtcttcgcc
     5221 tcttatttgc tgggcgagag tgacggaata gcgaaaaccg ccgaatgggc agcagagatt
     5281 tgtggtgtta acgcagagaa aatccgcgag ctggcggcta ttttccacca aaataccacc
     5341 atgctgatgg ctggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg
     5401 atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt
     5461 tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg ctgcggtact ctcttccatg
     5521 cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa tccctgtcgc ccgcattgtt
     5581 gaagcactgg aaaaccccgg tggcgcatat caacacaacg gtatggaccg acatttcccg
     5641 gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca ggataccaat
     5701 cgcctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg cttctggacg
     5761 gcggcggcaa aacacgcgga tatcgttctg cctgcgacta cctcttttga gcgtaatgat
     5821 ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg
     5881 ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttaagtga acgctgggag
     5941 aagggcggtt atgcacgttt tacggaagga aaaagtgagc tgcaatggct ggaaacgttt
     6001 tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc atttactgag
     6061 ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt
     6121 cgcttcgctg atttttgccg cgatccgcag gcgcatccat taaaaaccgc cagcggtaag
     6181 attgaaatct tctcgcagcg tattgccgat tacgcttatc cggattgccc ggggcatcca
     6241 atgtggctgg tgccggacga atggcagggc aatgccgaac cggaacagtt acaggtactt
     6301 tctgctcatc cggcgcatcg tctgcacagc cagctgaatt acagttctct gcgcgaattg
     6361 tacgcggtgg caaaccgtga gcctgtcacc attcatcctg acgatgccca ggcgcgcggc
     6421 atacaagatg gcgatactgt tcggctgtgg aacgcacgcg ggcaaattct tgccggagcg
     6481 gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat
     6541 ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat
     6601 ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc atggctagaa
     6661 aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc
     6721 aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa ccctgcgcct
     6781 ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatagc
     6841 gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc ttcggtgcga
     6901 caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga agcttaccgt
     6961 cttcttccca gacccagttt ttcgcattgg caagatacgc atcccgcacc agcggaatgc
     7021 actcgcgcca gtaattcact tttataaagg gatgccccca ggttgtactt tccagccaca
     7081 gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt tatttcccgg
     7141 atagcaacag cagccaacca catgatcatt caccagccca catgcctgca taaaggagta
     7201 acagattgtg gtaccgacaa acttaaaacc acgttttttc agtgccttag atagggcgtc
     7261 ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg gcttgtgtca cctgtggctg
     7321 atgatttaca aacgaccaga caaagtcggc aaacggttcg ccgttctgtt ccatttgcag
     7381 gtacgcccgc gcattaccaa taattgcctg aattttcccg cgatggcgga taatcccggc
     7441 gtcctgtacc agtctttcga catcctcttc ctgcattgcg gcaaccttca ccggatcgaa
     7501 ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataaccc
     7561 agcctgctgc ccttccaggc agatcatttc gaacagtttt ttcctgtcag tttcaggcac
     7621 gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc agccgcaacg
     7681 ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc
     7741 atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatacaatg
     7801 acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag
     7861 cggtggtcgc tggcagctaa gcctgctggc gagtgtggta atcagtgcct tttttctcaa
     7921 cacagcttac gcctggcaac aagaatatat cgttgatacg caacccgggc attccacaga
     7981 gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt cgcaacgtat
     8041 tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa cccctgtgga
     8101 tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg ttacaactgg
     8161 cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga
     8221 cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtacct taggctggcg
     8281 tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt
     8341 tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa
     8401 cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgccgc
     8461 ctatgccgcg ctatctcagg cagaaaatac cactaacaat agcgattatc tgtatacgat
     8521 gggggttagt gccagatttt aacgtaacaa tcacaattga aaccattgaa taacaatagt
     8581 tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat tgtcgcgtca
     8641 ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc gtttttcccc
     8701 aggcataaag tgcacttcgt tatggttgtc ggcagagatt tttccttttt attactgcag
     8761 gaatactgcc atgacacctt caaattatca gcgtacccgc tggttgacac tcatcggtac
     8821 tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta acggcgcgct
     8881 ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct
     8941 ggggctggca atatcgtctt ctgttgcggg caaattgcag gaacgttttg gtgttaaacg
     9001 cgtcaccatc gcttccggca ttttgctggg attagggttc ttcctgacag cgcattctga
     9061 caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg
     9121 ttacctgctg acgctctcta actgcgtgaa atggttcccg gagcgtaaag gtctgatctc
     9181 cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca
     9241 gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt
     9301 gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag
     9361 caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg
     9421 gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga ttggggtagc
     9481 gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tctgcagcca atgcagtcac
     9541 tgttatttcc atcgccaacc tttcaggtcg tctggtgctg ggcattctgt ctgacaaaat
     9601 cgcccgtatc cgtgttatca ccattggtca ggtaatttcg ctggtgggta tggcggccct
     9661 gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa
     9721 ctttggcggc actattaccg tctttccgtc actggtcagt gagttcttcg gcctcaataa
     9781 cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca tttgtggttc
     9841 gattatcgcc tcactgtttg gcggcttcta tgtgaagttc tacgtcatct ttgctctgct
     9901 gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaag tattgcgtga
     9961 ggcgcatatt tagccagaaa ctacaggttt tgaacgaaag ggggaggaaa tcctcctttt
    10021 ttattgcatt ttaaagaata aaaatgaaaa tattttatct tattatttgc gtggtgaatg
    10081 tttttgtttt gtttaattgg tattttatat ttatttattc ctaaaaataa acgatagctt
    10141 tttatg
//
LOCUS       DAZFTN010000148        10143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFTN010000148 DAZFTN010000000
VERSION     DAZFTN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..164)
                     /locus_tag="ACHKV2_004377"
                     /pseudo
     CDS             complement(39..164)
                     /locus_tag="ACHKV2_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(440..874)
                     /locus_tag="ACHKV2_004378"
     CDS             complement(440..874)
                     /locus_tag="ACHKV2_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6942140.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(867..1061)
                     /locus_tag="ACHKV2_004379"
     CDS             complement(867..1061)
                     /locus_tag="ACHKV2_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO6942141.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(1058..1621)
                     /locus_tag="ACHKV2_004380"
     CDS             complement(1058..1621)
                     /locus_tag="ACHKV2_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO6942142.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(1629..2078)
                     /locus_tag="ACHKV2_004381"
     CDS             complement(1629..2078)
                     /locus_tag="ACHKV2_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO6942143.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            complement(2078..3049)
                     /locus_tag="ACHKV2_004382"
     CDS             complement(2078..3049)
                     /locus_tag="ACHKV2_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO6942144.1"
                     /translation="MRTRDAAVGHWSRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMNLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVIDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSCNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            complement(3039..4559)
                     /locus_tag="ACHKV2_004383"
     CDS             complement(3039..4559)
                     /locus_tag="ACHKV2_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO6942145.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYTREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAN"
     gene            complement(4553..4930)
                     /locus_tag="ACHKV2_004384"
     CDS             complement(4553..4930)
                     /locus_tag="ACHKV2_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942146.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTILESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            complement(5138..5407)
                     /locus_tag="ACHKV2_004385"
     CDS             complement(5138..5407)
                     /locus_tag="ACHKV2_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942147.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            complement(5462..5665)
                     /locus_tag="ACHKV2_004386"
     CDS             complement(5462..5665)
                     /locus_tag="ACHKV2_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6942148.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGTVEAEEVRPFPAFVRGDD"
     gene            5761..6474
                     /locus_tag="ACHKV2_004387"
     CDS             5761..6474
                     /locus_tag="ACHKV2_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO6942149.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            6569..8038
                     /locus_tag="ACHKV2_004388"
     CDS             6569..8038
                     /locus_tag="ACHKV2_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFO6942150.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGLGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSTIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            8035..8988
                     /locus_tag="ACHKV2_004389"
     CDS             8035..8988
                     /locus_tag="ACHKV2_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFO6942151.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            9617..>10143
                     /locus_tag="ACHKV2_004390"
     CDS             9617..>10143
                     /locus_tag="ACHKV2_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6942152.1"
                     /translation="MGPTSVGLVPLIASCSNPVQSTASELATSSGGYQSPIKEAAIMA
                     TIPVLSQPEITISNGRAVTTSFAVAEYFCKRHDNVLRAIANIECSEKFNALNFEDVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEH"
BASE COUNT         2492 a         2735 c         2352 g         2564 t
ORIGIN      
        1 gcgcaggata ataacggtac gtaactaatc ctgcaatatc atccgttctg actgactaaa
       61 tcctgaactt ccctgaccgt ctgctcaaaa cgttcagtct ccagctcaac gccaattgca
      121 cgacgcccca gcgacattgc tgctttgacg ctacggacat aaaaaagcca gccactgggg
      181 gaggctggca aactcgtaga gcaaaatgct gttacgcaaa cttcgttaca gggtcatcct
      241 gcaatacaaa aaatacacaa tatttagaaa actaatagtg ccatgtgcaa tttttaagat
      301 tttgttatta attgtggtcg caccttcctt tctgtgtact ttccgtatag ctcacaggat
      361 tctgggtaca aaaaaacccg cgcatcggcg ggttaagcag cgtggcaatg taaccactct
      421 tatcatgata tgcagatttt tacgatcgta aactattttt tcgctgataa aatacagagg
      481 ttctccctcc cggcaattca cgctcaacat accgatccat ctcaagcctc actcccagca
      541 tcatcagcat gccttcaaca atcccctccg ctttgtgaag gcgtttacct atacaggtgt
      601 cagagcaccc atgtttccgt gccagcgcca tgaacgtctc ccccaacacg taataatcaa
      661 ccagcaagtc atgcagatcg ctgttgttcc tgttaaggcg agccatacac ccgcatataa
      721 tcatcgcgtc atcgtcacaa cactgtggac gtgattttac tttttcgggg atcagcctct
      781 taaatccggc agcaatgggc gaccatgtaa catcctcatg gttatttgcc gcccatgccc
      841 cccagcgctc aagaacctgc cggatatcac gcatcagtat ctttacccca tccgcggtga
      901 accataagaa caccgttgac gatagcgtgt cttttccctt ctttatcgcc aatgtatttt
      961 ctgactgtgg cacgattgca gttcagtatt cgggccactt cggtctgatt tccatatgcc
     1021 tcaacaagca tgtcaggaat ggtttttacg gtgaacgtca tgcggcctca cttctgctgt
     1081 ttcgcaggtc tttaagtttc tgttgatact ctgccttgat cgccttgcac tcttcgacag
     1141 tccagcggtg gcgactatgg tttgattcga tttcgtctac tgcttcctgc ccgatgcggt
     1201 taatcagttc gacacgatac ggaacgagat ttccgctttt gtactggtta cacaccacgc
     1261 attgcttatg gatattgcgt tcatcaaatc tgagctgagg tgccgcagct gttgtacggt
     1321 aatggcctgc atcccactgg gctgaatcga acgtcccgca tgaaacacac ggcaaatgac
     1381 ggtcccgctc cctgatataa gcattcacag cctgttgtgc ctgcttaatc cagtaactgc
     1441 ggggctgtaa gtccctcctg cgcgctttca gcttgtctgc ctgctctttt gctgaagcta
     1501 tcgccttttc ttttctgacc agttccagtg cgcactgacc acagcaaact ttctgatatg
     1561 agcggaacgg cacaaacgag gagccgcaca ctttgcattt ttttggttta cgttgcttca
     1621 tcatcacctc agaaaaacga cagcaggcgg ttattcagtt caggattgtt tgagcgcccg
     1681 aacacatgtt ttaacgcggc attaatcatt gcgttgtagc aacgttcaaa ctcatcctga
     1741 tccatattcg cgtaactcag gctttttgcc cgatatctca cctcgcctct tatggttgtg
     1801 accacatcat agaacccggc aagaattgtc agatttttac gaaactcatc aaactgcgtc
     1861 gcttcatcag agaattcgta tccagcatgt tcagcgcacc agtactgaaa gcagaaatta
     1921 agaaaagcga acatcttccg gtgaaaagat gggttacggg taagttttgc ttccagtgta
     1981 tacagctcac cgtttttaaa ttttgccagt cggggtaaat cgcgctcata cgccggtaca
     2041 aatacgccat tagccgcctt gatcatctct atttccatca ggcagcctcc gctttgaatt
     2101 tgcgcgtcaa cacatgttca cgggcttcac acccctgaat gagcatgtcg ttaaaatccg
     2161 gcaagtcagg ccagcgaatg ctgactttct caacgtcatt gcaactcatg agattcttat
     2221 gcccacattt aaaggcagcc gccagacctg cgccgtgcgc atcattgtca gcaaagataa
     2281 tcaggtgatt aacgccaggt ggcgcaatga acttctccat aaaaccggag ttcattgttg
     2341 accacacatt gcagcggaag atttgacggc atgaaagcgc agtttcgata ccctcggcta
     2401 tccccagagt ggacgacacc ggatacaggc gtattgccac cgatttggca tgctgcaaac
     2461 caggcaactc ctgtagcgcg gtcatttttt tagccgcctc tacattcgcc ttgcgatcac
     2521 catccagcaa cgtacgatgc agatagcaaa gagaaccttt gtcatctgtt gcaatggcgt
     2581 aaattgcctg atattcgcgc ccgctggcga tctgacggtc acaaaaacgc acggattcgg
     2641 tcggtaactg gagtattccc ctcccctgca ggtacgcctc acctgttgta ccccgcagac
     2701 acgggagtcc ggcaaactta tctatgacca gctcccgctt tctgcttatc tctgttacag
     2761 gctggctgac tttctccctc ttccaggtat ttccgatcag ccgatcgatt tcatcacaca
     2821 acgtcaccca gggcttaccc gtggcaagtt gcaacagatt catcccgtcc ccgtgaccac
     2881 agacgcaaat ccatgaaccg gaaccatcct tatcatcaca gcgaaattta cctctggcac
     2941 cacatatcgg gcagggtccg ttgtaatgtt tcacaccagt aacaggaggc atgccgtaat
     3001 attcaaaaat ccggctccag tgccccacgg ctgcatctct agttcgcatg ctggcctcct
     3061 gtcatggctt tcttgcgccc tttggcatag gcaatggtct ttgacttgat gaagttgtac
     3121 acttcaactg aggtttgcag cggcgcatta ctgaagcctt taggccaaac cccgaacttc
     3181 tccttgtagg tatgagcaca ccatccgtca ctcagtggct tacctgtcgc gttgcggtaa
     3241 ttctgatacc ccttgatctc gctccaccaa cgctgttttt cctcacgggt gtattcgcgt
     3301 ttccctttgt tgacgcgtga aagcttacga tcgcgatctg tcgccacgtc atcaccacca
     3361 agaggacgaa atccacattt gggacacata tgcacgccag caggcttcat gaagtggcat
     3421 ttggggcatt ccctgggaag tttctcagcc ttaacctcac tcccgccagc agacgctttc
     3481 attccgtcat tttttcccgg aagttcgtca tactcgatat cctcggggaa gcccagccga
     3541 tgaaccgtgc cggaatggtc gaaaatcaat gcgcgttttt taccagacgc agtacgtaac
     3601 gctctcccga tgcactgcaa ccagcggatt tctgatttgg tcggacgggc gtagatcagg
     3661 cagcgaacat cgctgtcaaa tcccgcaacc agtacgccaa cgttgacaat tattttggtc
     3721 gcaccctctt caaaacggcg aatgatgtcc tgtcgttcat catggggggt atctgccgtc
     3781 atcacctctg cgccaatgcc agactgtaaa aactcacggg taacaaagtt cgcatgagcc
     3841 acattcacgc agaagcagat cgtcggtaaa tcctcgccat tctcaagcca gttgctgacg
     3901 atatcgccaa ccagatccga actccccatg atggatgcga gctgttcctc gttgtaatcc
     3961 cgtccaaata cggtgttact ggtttttacg ccagccagat caggcattga gggggcaaaa
     4021 aactcataat cgctcagatc accacgctga ataagttcac gaatcgttgt aggcttgatc
     4081 aggcattcgt agtacttccc catccaggct gcgaacgggg tgccggaaag cccaagaacc
     4141 ctgatatctt tgtcccggat aacctcaagt aacgcacgac gcttcatgtg tgcttcatcg
     4201 atgatcagca gatcgatgtt gtcaggaaac tcacgacgga tcagcgtgtc ggcactggca
     4261 atctgaatca gtcgtgacgg gtcatagtta gggtgatcac gccatacaaa actgatttct
     4321 tcccacggca aaccgtattc ggtgaaacgt tccgctgtct ggttcagcag gatggtgtac
     4381 ggacagacaa acatcacccg cataccacgt tcaaccattc cggcagtgac gaatgctgca
     4441 agaccagttt tgccagagcc agtgggtgca tacatcaggt acgtgcgatg ctgtttccac
     4501 tggtgccgca gctggtttaa accgcgctcc tgagcaaagt ttggggtgat tttcagcatg
     4561 acgccccctt gccattcgcc gggatgatat cgatcccagc atcgctataa ctggtcagtt
     4621 tctttggcat tcgaagcaac acaaagcctt cagtgccacg acgtgccaat tcacgcaatt
     4681 tcatgactga gcggggtcgc ggagagctac gatcgatctc gatagccaca ctcccaccag
     4741 tttttgctgt tgcgagaata tcaacacgga aatttctgcc atcgattctt actgtgacat
     4801 tgcttgccac atgcatcccc tgtgactcaa ggatcgttac aacatcagcc agaaagctgt
     4861 aacgctcctc tgttctcagt ggtacagtgc tcagtgtacg gatcagtgcc gatttggttg
     4921 ctgccgacat agtttatcac tcaatagtta gctcgataat catttcacca gaaactatcg
     4981 cgcgcttata cagtgatcta cctaacctat gtacctgcct tctggcctag gccaagggat
     5041 acaggcctta ccaacgactc caccccctta ccccctcctc gcttcctttc aggaaattag
     5101 ttgcacttga aattatctct agcgcaacta accgccatca tggtgtcggg ggaaacttcc
     5161 gtaccgctac tggcgaaagc atccagccac ggctggctct cgcgtacttc tggacataca
     5221 cccgtaaccg ggtgttggcg ctccgtcttg ccctgttgcc cttcctgaac gatacaggct
     5281 ccttgtccca ctcttcctgg tacactctcg cgtactctgc tgcaattttt acccgggtgg
     5341 tcgggtctag ctgtaacaat tgctcctgta tccagtcccg atcagcatca cagtactgct
     5401 cgggcataac cgttttgatt tgcctgttca ctcacacctc cgatgggctg tgtgtccagg
     5461 attaatcatc gcctcttacg aacgcaggga acggacgaac ctcttccgcc tcaactgtgc
     5521 cgtcatcaaa ggttttgacg tatatttctc gtccgacgag aagcgcttta ctgattgcac
     5581 tctgcgccac gccaaagtcc ttagcggctt tcttttggcc gaacctgtat acgtaatcag
     5641 ccagggtctc ttttttcatc gccataactg actccttcaa aatgcccata tcattacgca
     5701 cagttattaa aatgtcaaca ccgcagatat ttcattacat ataaccaaag gtgatatggt
     5761 atgaacatga aaaagaaacc gttaacccct gagcaactgg aagacgccaa gcgtctgaaa
     5821 agtattttta acgctaaaaa gaaagagtta ggcctgtcgc aggaatcatt agcctatgaa
     5881 ttaggtgtta cccaaagcgc tgttaatcag ctaatggctg gcatcaacgc cataaatgcc
     5941 agccacgcag cacagcttgc taagatactg aatgtaaagg tcggagattt cagtcccagc
     6001 ctggcaaaat ctattgcaga aatggcgctc gcaattgaag agccattaac tcgtgtcccc
     6061 gcttacgaat atccgttact ctcctgcgta caagctggcg cattctcaat ggatgatatt
     6121 tcgtacaccg caaaagatgc gattaaatgg atctccacca ccacaaaagc cagtgacagg
     6181 tcgttctggc tggaggtcaa ggggcattca atgaccgcac cgcagggtgg taaacccagc
     6241 ttccctgaag gtatgctgat actcgttgac cctgaacggg aaatcgagga tggcgatttt
     6301 tgcgtggccc gaatgaacgg cgatgaattt accttcaaac gattcattcg tgagagcggt
     6361 aaagcgtacc tggagccgct caacccacga ttcgacatga ttgagtgtaa cgaaaactgc
     6421 cagtttgtcg gaaaggtcat caaatcgcag tggaatgatg agacttttga ttgagctcac
     6481 agagtgattt gacatccttc acgcctgaag aatgtggagg atgtcaaaag cacgttggca
     6541 tcatcatgcc acgtaaagag gattagttat gcttcaaaag ctagacgtcg cagacagcgt
     6601 gcgtcgcgag ctgtctcccc gtaccgcgcg taaacacaaa gcagagttcg gccagttcat
     6661 gacgccttct ggcgtggccc gttttatggc ctccttgttt ccaccgagca caatgaaaac
     6721 ttgccgtcta ctggacgcag gcgctggttt aggcgcactg tcttgcgcct tcctcgaccg
     6781 ttggctggtt ggcgagtttg gctttgagtc tgtcgaggca acagcctacg aaatcgacaa
     6841 aaagctgtgt ggtcaccttg cgaagcactt aaccgggtac aggagagtga cgccccgcat
     6901 catcgaaggg gattatattg agctggcaac cgccgaaggt ctacaggatc gaggttatac
     6961 ccatgcaata cttaatccgc cttacaagaa gatcaatagc cagtcagccc accggcaggc
     7021 tcttcgcact gtcggtatcg agacggtaaa tctgtattcc gcctttgttg cactggctgt
     7081 aggtgaggtt gcacctggcg ggcagatagt ggccataatc ccacgtagtt tctgcaacgg
     7141 accgtattac cgtccgttcc gtaatttcat acttgagcgg gcagcaatcc gccatattca
     7201 tttgttcgaa tcacgtagca aagctttcag ggatgacgat gtgttgcagg aaaatattat
     7261 catccgcctg gagcgtggag gccgtcagga ggctgttaca gtcacaactt cgaccgatga
     7321 tagctttttt gacctagtca cctacgaaca cccatttgac cagattgtgt acccggacga
     7381 ttcggagcgg tttattcatg tgccgaccac gctcgagaaa agcaccattg agttatcatc
     7441 tgccgtacaa tgttcgctgg ctgatattgg cgtgaaggta tcgaccggac cgatagttga
     7501 tttccgactg aaagctcatt tgcgcagcat gccagaagaa ggaactgttc ccctgatcta
     7561 cccgagccat ttgagtatga gtagcactgt ctggcctgtg gaaggtttga agaaacccaa
     7621 cgcaatcatg cgcaacgacg agacagaaaa gtggctttac ccgaacggtt tttattgtgt
     7681 ggtgcgtcgt ttctcgtcga aggaggaaaa gcgccgagtg gtagcaagcg tcgttgatcc
     7741 tgctaccttc agtgagtatt cagtgttggg atttgaaaat cacatgaacg tgtttcatga
     7801 gaacaaacat gggttacccg aggcgctggc ccgtggcttg gccgtgttcc tgaacactac
     7861 cgcagtggat aagtatttcc gtcgcttcaa cgggcataca caggtcaatg caaccgacct
     7921 caagatgatt aagtacccaa gccgtgacac tctgatagag cttggcaagt gggctatgca
     7981 acaagaaact ctcacgcaag aacatattga cgctaagcta ggagctctga ctgcatgaac
     8041 aaccaaaatg attatattga ggccgctcag caaattatag catccttagg cctgcccaga
     8101 gcgcagcaga atgagcgttc tgctttgtgt ttgctggcac tcctgaacct cacaccggga
     8161 aaagcatggg ccgacgcgga aaatccgctt gtgggcatta caccaatcat gaattgggtt
     8221 cgggagcact acggcaaggt ctacgcaccc aatactcgtg agacattccg ccgccagtct
     8281 atgcatcagt tctgcgctgc tggagtagcc ctctacaatc cggacaagcc cgatcgccca
     8341 gtgaacagcc cgaaggctgt ttatcagatc gaacctgctg ctctgtccat gctacgcact
     8401 ttcggcagcc cggcatggca cgatagcctt gcaacctatt tagccgagcg tgaaacgctg
     8461 gttacccgct atgctaagga gcgcgaacag aatcgtatcc ctgttgaaat tgctgcgggc
     8521 cagcaaataa ccctcagtcc cggcgagcac agcgaactta tccgtgccat aatagaggac
     8581 ttcgccccac gttttgctcc gggtagcgtg ctggtctatg ctggcgacac tggcgaaaag
     8641 tggggctact tcgacgctcc actgctggcc gggctaggtg ttgatgtgga ttcacacggt
     8701 aaaatgccag atgtggtgct acattttact gcgaaaaact ggctgctact ggttgagtct
     8761 gtcaccagtc atggaccggt tgatgggaag cggcatgctg agcttgccag gctgtttgcc
     8821 ggatcaacag ccggacttgt ctatgtgacc gcttttccaa atcgctccat catggggcga
     8881 tatcttggtg aaatcgcatg ggaaacagag gtatgggtgg ctgatgcacc ttctcatctt
     8941 attcacttca acggagtgcg atttcttggc ccctactcta ctgaatgaga acaggaccag
     9001 ataaatagcc cggcccatgc gccgggtttt tctttgcata tcccctcctg ttatctgccc
     9061 ccttaaaaaa taaatcactt taaaaatcag ttaagtaata atttctcgcc cttaaaaagc
     9121 accaacggta tttacatgaa aatcaccgca agttatattt atttcaagcc agaaacatcg
     9181 cgcccttaca cagggctaca taaatcagtc gtacggcgcg acttaacccg ccgcaaaatg
     9241 ctctttaaca atctggagct ttacagcgtc aatgacctgt ttagacccct acacgtaaac
     9301 gtgctgtatc atcgggtgcg atccggtcga tgagagagta tccccgcgcg agagcgagaa
     9361 cggcgtgaga acgggcaaca ctggcaggaa gttggcgctg accaatacag ggaatgtttt
     9421 ggggtgcagg cggcaccgcc aaagcgtttc tgagaaagaa cggtattgac tgcgtgtgat
     9481 gtcggggcta tatttggcga acacctcata aaacgggtgt cgggattgct accccgctga
     9541 caatcgaggc gatacagacg cgccagcgtc tttttttgta tcgacataca cgcacatctt
     9601 atcaatggtg ggctgtatgg ggccgacttc ggtcgggctg gttcccttga ttgccagttg
     9661 tagcaaccct gtacagtcca ccgccagcga gcttgctacc tccagcggtg gttatcaatc
     9721 accaatcaag gaggctgcca ttatggctac tatcccagtc ctttctcaac ctgaaatcac
     9781 catttccaat ggtcgcgctg ttactacgtc ctttgccgtt gccgaatatt tttgcaagcg
     9841 acacgataac gtattgcgcg ccattgcaaa tattgaatgc tcagagaaat ttaacgccct
     9901 caattttgag gacgttacct acaccgacgc caaaggcgaa caacgcccaa tgtaccaaat
     9961 caccaaaaac ggcttcgttt ttctggtgat gggcttcacc ggcaaaaaag cagctgcatt
    10021 caaggaagcc tacattgctg aattcgatcg catggaaaaa caactatggg aacagaaaca
    10081 actggccctg tcttctcctg cgatgaatat caatttccct ttgtcgtggt tttcagagca
    10141 tca
//
LOCUS       DAZFTN010000149        10079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFTN010000149 DAZFTN010000000
VERSION     DAZFTN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..821
                     /locus_tag="ACHKV2_004391"
     CDS             <1..821
                     /locus_tag="ACHKV2_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942153.1"
                     /translation="ESPKQSLLNDFSHRLKMEGEEQLFHCVNELKYGLSSNNEYDLFK
                     NRETSLWKRKVPESAALAMTGTVYKLFGSRLLNARNQLDKNLLEMAMQMVYGKDMVTK
                     SGDILIDIQLNKDGSLQSKHYTFDVGEVINSYNIDLFVNASSINQTYLHDKNPGDVIS
                     LYGTLDNIPLVVKECTGKNHQINVKAKLPQERSEKVEDMCERMRGGISMFNHTTKTAG
                     NIEHNLQLSFLVDSRPSITNKYSAKDVAPDILVLPVNVYHADFKIKINNAVEKT"
     gene            828..1742
                     /locus_tag="ACHKV2_004392"
     CDS             828..1742
                     /locus_tag="ACHKV2_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6942154.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2436..2654
                     /locus_tag="ACHKV2_004393"
     CDS             2436..2654
                     /locus_tag="ACHKV2_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6942155.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2871..3113
                     /locus_tag="ACHKV2_004394"
     CDS             2871..3113
                     /locus_tag="ACHKV2_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6942156.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3443..4372)
                     /gene="fdhE"
                     /locus_tag="ACHKV2_004395"
     CDS             complement(3443..4372)
                     /gene="fdhE"
                     /locus_tag="ACHKV2_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO6942157.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4369..5004)
                     /gene="fdoI"
                     /locus_tag="ACHKV2_004396"
     CDS             complement(4369..5004)
                     /gene="fdoI"
                     /locus_tag="ACHKV2_004396"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6942158.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5001..5903)
                     /gene="fdoH"
                     /locus_tag="ACHKV2_004397"
     CDS             complement(5001..5903)
                     /gene="fdoH"
                     /locus_tag="ACHKV2_004397"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO6942159.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(5916..8966)
                     /gene="fdnG"
                     /locus_tag="ACHKV2_004398"
     CDS             complement(5916..8966)
                     /gene="fdnG"
                     /locus_tag="ACHKV2_004398"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8379..8381),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6942160.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQENYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9160..9993
                     /gene="fdhD"
                     /locus_tag="ACHKV2_004399"
     CDS             9160..9993
                     /gene="fdhD"
                     /locus_tag="ACHKV2_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO6942161.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2547 a         2422 c         2569 g         2541 t
ORIGIN      
        1 atgaatcacc gaagcaatcg ctattaaatg atttttcaca tcgccttaaa atggaaggtg
       61 aggaacaact gttccattgt gttaacgaac tgaagtatgg tttgtcgagt aataatgaat
      121 atgacctgtt taaaaataga gaaacctcac tgtggaaacg caaagtacca gaaagcgcag
      181 cgctggcaat gacgggaaca gtatataaat tatttggtag tcgcttactc aacgcccgca
      241 atcagttaga taaaaatctc ctcgaaatgg ctatgcaaat ggtttatggc aaagatatgg
      301 tgacaaagtc cggggatata ttgattgata tacaattaaa taaggatggc agcctccaga
      361 gtaagcacta cacatttgat gtaggtgaag ttataaacag ttataatatt gacctctttg
      421 tgaatgcttc gagtattaat caaacttact tacatgataa aaatcctggt gatgttatta
      481 gcctgtatgg cactttagat aacattcctc ttgtagttaa ggaatgcacg ggtaaaaatc
      541 atcaaattaa cgttaaagcc aaattgccac aagaacgaag tgaaaaagtt gaggatatgt
      601 gtgaaagaat gagaggtggc atctctatgt ttaatcacac cacaaaaaca gctggtaata
      661 tagagcataa tttgcagcta agttttctcg tggattcacg gccgtcgatc accaataaat
      721 actcagccaa agatgtagcg ccagacatac ttgtattgcc cgtcaacgta tatcatgcgg
      781 attttaaaat taaaatcaat aatgcagtag agaagactta gtcaattgtg ttatgtgctg
      841 tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg gataataggt aatgtaccaa
      901 aaaataataa accaaaaaca atcatttttg atttacgttt tcttagtgca attaagcaga
      961 aagttttttt gggcagtgaa caaccgttgt ttgtaaagca taccgcaatg atgagggagg
     1021 catgtaagga attaccgcag ctagtggagt atgttccttt agcctgtgaa gcgcatagcc
     1081 atcgcagcgt cgcccttgcc aaaaccgtgg ctgcacccat catggcgaca ggtgtgggcg
     1141 cggctttaat gtatgcaaca tcatcaggac cgtattatgc gcaaagtctt tctggttcgc
     1201 cggatatcga gatgataaaa aaacgcatgg ctgaattgtt taaccagtcg gatacttctg
     1261 tgcgcaacaa atatcgcccg gcttttgata aatggaataa actggtagat aaattaaatc
     1321 acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat tatagcgaca gcgataaatg
     1381 aaactcctga gggggatacg aatgttgccg ttgtgatggg gtgtaaatct gcgaaagatc
     1441 gcacgatatc cattgtcctt ggcaactcga tgttgcaaac actctttgaa aaacgcctcg
     1501 ccgatgatgg agaaatagaa aacctttttg atcaacaggg atattttagt tgcgattcat
     1561 taacagccga agaagtaatg atgttaaaag acctttttga tattcgagtt cttcatctct
     1621 ctaacaaatt taatgttggg ttgcaaggta atatcaacac ggatgtttta caagattcgt
     1681 tttttaaaaa tgtcgatttc attcatgaat ctaactcgta taccgtaggg atgggagtct
     1741 gaagagtaaa actcattagg attatcagaa ttcggccaat gcattgcaaa aatgaaatac
     1801 aaagagagat gtgtgcaggc gcttagccgc gaggggcatg gcgaagccat acagaaagca
     1861 ccggcattca gaactcctgt gaaagcggca tagtgccgct gctcaggagt tttgttatga
     1921 cccgtatcgt gctgaacgtt gccaccctat gatgcgatta aaaaaactca ccgtcgagcc
     1981 gatgattgac gtcaatgcta tcggatgatg ccgggggtgt tccggaagga attccttgtt
     2041 tcccgaactg ttaccaacaa aattataacg atgagcaagt aaagaccggt tggtggcagt
     2101 actgaccggt ctgtgatgga agattaattt caccttaaat cctggtgctg ttaggatgct
     2161 gacgtttttc gggtacacca ttcagattat gcaaataaag attcaatgaa aaaataaata
     2221 tccctgaata taatatattc gtagcaccca taactcatcc tgggcgataa ctaaaccatt
     2281 tttgagtaac aaatgaaaca actgcaacgc tcttattatt tctggaaaac gtctcgcaaa
     2341 aacaaaacaa catgcaaaaa tccgctttcg ttaattaatt tttcggcgta attttcgcac
     2401 atacattaat gagtaatata tgtaaggtgg tggtaatggc gatgaatacg gtttttcttc
     2461 atttatcaga agaggcaatt aaacggctga acaagcttcg gggctggcgt aaggtttcac
     2521 gatctgcaat tttacgcgaa gcggtagagc aatatctgga acgtcagcaa ttcccggtgc
     2581 gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg tgttgatgat cagtgtaagg
     2641 agcataagga atgacaaaaa tttttcattc ttgaatataa aaaacagatg cccttattct
     2701 ggtattaata gaaggctgtt ttacttgaac ttataataac tgcaactgtt acatcatatc
     2761 tggaaaacgc ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcga
     2821 caagggtact tttctctcca tagatatcaa atgatattgg tcaaatattt atgaactctc
     2881 tggcaggtat tgatatgggc agaattatac tcgatttatc gaatgaggtg attaagcaac
     2941 ttgatgatct tgaggtgcag cgtaatcttc ctcgtacgga gctattacgg gaagcggtag
     3001 atcaatactt ggtaaatcaa tcgcaaacag taagaaccag tgcttttggc atatggcaag
     3061 ggtgtgagga agatggtgtc gaataccagc gtaagctgcg cgaggaatgg taatgggaaa
     3121 aaatgatatc aatcagattg ctgacaacgt gcgcgttgtt catgccggat gcggcgtgaa
     3181 cgccttaccc ggcctacaaa gtcgtataaa ttcaatgtat tgcagtttat tggtaggcct
     3241 aataagcgcg gcgcatcagg caatcttacg cttatcatca gtctcatacc cataacctta
     3301 ttgatgtggg ggtgggtaaa ttgaggcaaa acaggcactg gcggcatagc ttgaggtggt
     3361 aacgcggaat acgagagatg gtgtcgtcac accctatgag ctgtaatccg ggcgaccagc
     3421 gccacccgga aaacgtcaga gattactccc cttcacccgg aaacaggaac gggttgatgg
     3481 aactgcgggc atagccttct tgctccattc gcgcgtccag taccagagag gcgaggtcat
     3541 ctgccacggc ttcaactttc ggttcttttt cctgatagag aattttcagg taagtgccgc
     3601 agtcatcgca gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca
     3661 gtttgccgct ctgctcgcag ttgctgcatt ttacgcgcac aacgtgccat tcagtttcac
     3721 acaggttgca gtgcaggtaa cgtagcccct gagtggtgcc aatttgcacc atgctggaca
     3781 ccggcataga accacaaacc gggcaatatt gacgttgctc gccgtattca gcgcgggctt
     3841 tgccggggat cagattggcc atctgcgccc agtagagcga cagtgcagcc cagataaacg
     3901 gcgctttatc gctgctgacg gacgagaaat cagaggcaaa cagtgcgctg gccatatctt
     3961 ccagctcctg agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca
     4021 tttcaggttt cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg
     4081 gcagaacgtg aatatccagc gggggcttgc cttgtgcgct ggcttcttta atgcgcgcag
     4141 tcagatccat ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg
     4201 cagcaaagcg caggtaatca cccagcggat tattttctgc cagctcgcgc agacgctcgg
     4261 cgcggcggtt gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg
     4321 tacgtttctc gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt
     4381 cttttccgtt gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaagc
     4441 gctggtaacc catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta
     4501 gatatgcacc atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc
     4561 gaatcggatc accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc
     4621 gctcaccagc agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa
     4681 gttataacgc ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc
     4741 ccaaaagata tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat
     4801 gaacgaggca aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt
     4861 gcccatgatt tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc
     4921 cgccaggatg aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg
     4981 cacgatggtg tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc
     5041 tcttcctcat ccgcatggtt cggaccgaca cccacgtagt ggaagatact ggctgcgaag
     5101 gtagccgcaa agccaacagc tgcgagcggt ttccagatgc ctttccagaa tttcacggtt
     5161 tcgctgattt ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc
     5221 agcacgtaca taacgtgtgt accaccgacg cctgccggat cgtacaggcc cgcattgtcg
     5281 taaccgcggg ttttcagctc cgccacgcgc tcgctcgcca gcgttttcat cgactctttc
     5341 gtaccgaagt gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc ttgcccaacc
     5401 accacgcggt caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc
     5461 ggaatgtcga acggacagcc cgcaatgcaa taaccgcagc caatgcactg ctcggactgg
     5521 aagtcgacga taccgttggc atactgaata attgcacctt ccgccgggca cgctttcaga
     5581 cagcctggat cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg
     5641 ttctgctcca cttccgagaa gcgcatcacc gtccacgatt tggcgcttaa atcattgggg
     5701 ttgtcgtaca ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac
     5761 gccacctgac aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct
     5821 tcctggaagt cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg
     5881 atatcttgag attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg
     5941 aaggacttaa actccggcgt ctgcgtgttc gcatcaccga cgaatggcgt caacgtattg
     6001 gcaataaagc cttttttcgc aacaccttca tagccccagt gaataggaat accgatggta
     6061 tcgatatctt tgccgttcgc tttcagcgtg cgaatacgtt tggtcaccac cgctttggct
     6121 ttgatatagc cacggttgga ggagactttc acggtatcgc cctgggcaat gccaagttta
     6181 ttcgccagcg actccccgat ttccacaaac tgctctggtt gcaaaatcgc gttcaacagc
     6241 gcgtgtttgg tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg
     6301 aacttatcgg ctttacccaa tgcttcggcg tcgtctttaa agatacgtgc agccgggttc
     6361 gagataacgt ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt
     6421 tccgggaacg gaccttccgc catcttatcg agggcaaaca gacgccccat gccttcctgc
     6481 tgcatgataa acggcccgac gccgctaccc ggaggcgctg cgctgtagtc cggaatatcc
     6541 cagccggtcc acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta
     6601 ccctgcggat ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat
     6661 gcccaaccca gcgtgttacc gaggccagac ggatcggcgt tatcacggcg tgccatctgg
     6721 ttgccttccg gcgtccagct accggcgaaa atccagcaac cacaggaagt tgtaccgtca
     6781 tcgcgcagtt gggcaaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt
     6841 gccggatcgg taatatcggc cagcgcctta ccgttgctct ccatcgctac ttcttccgac
     6901 ttcggctcat gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggttcgcg
     6961 ccaccctgtt cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgcca
     7021 tcagtcagcg caatccccgg ggcgtccgca cctttccagt gccactgcaa ccagcgacct
     7081 gagttaacga ttgaaccgtt ctcttctgcg aagcaggtcg atggcagacg gaacacttcg
     7141 gtctggatct tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag
     7201 gtttcagtgt tcagcgggtc gatggtgacg aggaacttca gtttcgacag acaactgatc
     7261 actttgtttt tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg
     7321 accttgcctt ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc
     7381 ggcaaccagt caaagcccca gctattttct gccgtcgctt tatcaccaaa gaaggccttc
     7441 atcatagaga cgaagaattt cgggtagttg ccccagtagt tttcctggcc ttccagcagc
     7501 ggttttggcg tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc
     7561 gtcatgtaac ctggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg
     7621 gagtgaccgc gcagggcgtt aacgccgccg ccagccatcc ccatgttacc gagcagcagc
     7681 tggatcatcg ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt ccagccgagg
     7741 gcatacagga acgaggcagt tttatcgtga gcactggttt ctgcgatgta ttcgcagact
     7801 ttcaggaacg cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg
     7861 gaaacgtgct gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt
     7921 ttggcgaagc cgttttcgtc cagttcataa gtccaggagg atttatcgta cttgcgtttt
     7981 tccgcgtcgt agccggtgaa caggccatct tcaaagccgt aatcctcacg cacgatcagg
     8041 ctggcgttgg tataggcttc agtgtattcg cggttgaatt tttcattgtt cagcaggtac
     8101 agcaatacgc ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca
     8161 gccaccgcag ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccattgtga
     8221 attttggctt ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc
     8281 atcaccacga cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga
     8341 ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg
     8401 accgcgagca tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc
     8461 gacgcggaag cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg
     8521 ttttgcgcaa tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac
     8581 gcctcttccc aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac
     8641 ttcagacggc tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca
     8701 ccgcggttga ccgggtgatc cggatcgcct tcgatatgga agatagatgc tttggcgttt
     8761 tttgctccgt caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag
     8821 gtattacggg tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagtgctacg
     8881 ctgggtgcaa aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta
     8941 aagaactgcc ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca
     9001 tttacattcg cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc
     9061 gcaaaggtcc agaattggct caatttccct ccatccagga agggtttgta acagaatacc
     9121 ataatgttgg tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa
     9181 gaaatcgaaa atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat
     9241 gatttacaac acccacggtt agatgaggtc gcggaagaag ttcccgttgc gctggtctac
     9301 aacggcattt cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc
     9361 ggtttttcgc tttccgaggg gataatagaa agtccgcgcg atatcttcgg catggatgtc
     9421 gttccttcct gtaatggtct tgaagtacaa attgagcttt ccagccgccg ctttatgggg
     9481 ttgaaggagc gccgccgggc gctggcggga cgtacgggct gtggcgtatg cggcgtggag
     9541 caacttaatg acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc
     9601 aacaaactgg atgatgcatt acgtcatctc aacgattttc agccagtggg acaactgacc
     9661 ggttgtactc acgccgccgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa
     9721 gacgtgggtc gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa
     9781 agctggcagc aaggtgcggt actggtttcc agtcgtgcca gttatgaaat ggtgcaaaag
     9841 tcggcgatgt gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac gcttgctgta
     9901 gaagtggctg agcgctgtaa tctgacactg gtaggttttt gtaaaccggg tcgggcaacg
     9961 gtttataccc atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatatatta
    10021 aattgcagga cttattcatt tcgtgaattt tattatttta tttataaaca aaatgcgtg
//
LOCUS       DAZFTN010000150        10023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFTN010000150 DAZFTN010000000
VERSION     DAZFTN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            381..1358
                     /gene="glaH"
                     /locus_tag="ACHKV2_004400"
     CDS             381..1358
                     /gene="glaH"
                     /locus_tag="ACHKV2_004400"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO6942162.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1378..2646
                     /gene="lhgO"
                     /locus_tag="ACHKV2_004401"
     CDS             1378..2646
                     /gene="lhgO"
                     /locus_tag="ACHKV2_004401"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO6942163.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2669..4117
                     /gene="gabD"
                     /locus_tag="ACHKV2_004402"
     CDS             2669..4117
                     /gene="gabD"
                     /locus_tag="ACHKV2_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6942164.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4131..5411
                     /gene="gabT"
                     /locus_tag="ACHKV2_004403"
     CDS             4131..5411
                     /gene="gabT"
                     /locus_tag="ACHKV2_004403"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO6942165.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5649..7049
                     /gene="gabP"
                     /locus_tag="ACHKV2_004404"
     CDS             5649..7049
                     /gene="gabP"
                     /locus_tag="ACHKV2_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO6942166.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7070..7732
                     /gene="csiR"
                     /locus_tag="ACHKV2_004405"
     CDS             7070..7732
                     /gene="csiR"
                     /locus_tag="ACHKV2_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO6942167.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7733..8182)
                     /gene="kbp"
                     /locus_tag="ACHKV2_004406"
     CDS             complement(7733..8182)
                     /gene="kbp"
                     /locus_tag="ACHKV2_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO6942168.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8266..8424)
                     /gene="yqaE"
                     /locus_tag="ACHKV2_004407"
     CDS             complement(8266..8424)
                     /gene="yqaE"
                     /locus_tag="ACHKV2_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6942169.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8607..8906
                     /gene="ygaV"
                     /locus_tag="ACHKV2_004408"
     CDS             8607..8906
                     /gene="ygaV"
                     /locus_tag="ACHKV2_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6942170.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8916..9434
                     /gene="ygaP"
                     /locus_tag="ACHKV2_004409"
     CDS             8916..9434
                     /gene="ygaP"
                     /locus_tag="ACHKV2_004409"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO6942171.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9487..9891)
                     /gene="stpA"
                     /locus_tag="ACHKV2_004410"
     CDS             complement(9487..9891)
                     /gene="stpA"
                     /locus_tag="ACHKV2_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO6942172.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2296 a         2561 c         2732 g         2434 t
ORIGIN      
        1 ttttagggaa aacaggagaa ccgctgaact atctctattt gtaatttaat gttatatctg
       61 ccccgctaaa acggggcaga taatatattt agtttactag cggtcatttt gcagtgaagc
      121 catttaccgt tttttatcga ccagataatc tgttctctga tggtaatccc cccctaacct
      181 gttgctttag ttattcattt cctgtctcac tttgccttaa taccctacgt taaatgttac
      241 caatttgttg ctttttatca caataagaaa acaatatggc gcatttgtgc gcatttttca
      301 gaaatgtaga tatttttaga ttatggctac gaaatgagca tcgccatgtc accctacatc
      361 tcataagagg atcgcttctg atgaatgcac tgaccgccgt acaaaataac gctgtcgatt
      421 cagaccagga ctatagcgga ttcactctca tcccgtcggc gcaatccccg cgtctgctgg
      481 aactcacctt caccgaacag acgaccaaac agtttctcga acaggttgcc gaatggcccg
      541 tgcaggcgct ggagtacaaa tcgtttctgc gttttcgggt aggtaaaatt cttgacgatc
      601 tgtgtgcgaa ccagctgcaa ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg
      661 cgctgttgat caatgcggtg ggtgtcgatg atgtgaagca agcggatgag atggtgaagc
      721 tggcgacggc ggtggcgcat ctgattggcc gttccaattt cgatgcgatg agcggtcagt
      781 attacgcgcg ttttgtagtg aaaaatgtcg ataactcaga cagctatctg cgtcagccgc
      841 accgcgtaat ggagctgcac aacgacggca cctacgtcga agagatcact gactatgtgc
      901 tgatgatgaa aatcgacgag cagaacatgc agggtggaaa ttcgttgctg ctgcatctcg
      961 atgactggga acatctggac cactatttcc gccatccaat ggcgcgtcgc ccgatgcgct
     1021 ttgccgcgcc gccgagcaaa aacgtcagca aagatgtttt ccatccagtg ttcgacgttg
     1081 atcaacaggg tcgcccggtg atgcgctata tcgaccagtt cgtccagcca aaagacttcg
     1141 aagaaggcgt gtggttgagc gagctttcgg acgccattga aatcagtaaa ggcattcttt
     1201 ctgtacccgt tcccgttggc aaattcctgt tgattaacaa cctgttctgg ctgcacggtc
     1261 gcgaccgctt tactccgcac ccggatctgc gccgtgaact gatgcgtcag cgtggctatt
     1321 tcgcttacgc cactcaccac tatcagacgc atcagtaagc gcaaaggaat tgagcggatg
     1381 tatgattttg tgattattgg cggcggcatc atcggcatgt cgaccgccat gcaactgatt
     1441 gatgtctatc cggatgcccg cattgcgttg ctggaaaaag agtccggccc ggcctgtcat
     1501 cagacgggcc acaacagcgg cgtgatccat gccggggttt attacacgcc cggtagcctg
     1561 aaggcgcagt tttgcctggc gggaaaccgc gccactaaag ccttttgcga tcaaaacggc
     1621 attcgctatg acaactgcgg caagatgctg gtcgccacct ctgaactcga aatggaacgg
     1681 atgcgcgcgt tgtgggaacg cacggcggca aacggtatcg agcgcgagtg gttaaacgcc
     1741 gatgaactgc gcgagcgcga accgaatatc accgggcttg gcggtatttt tgtgccgtcc
     1801 agcggcattg tcagctaccg cgaagtcacg gcggcgatgg caaaaatttt ccaggccaga
     1861 ggcggcgaga ttatctataa cgccgaagtc agcggcctta gtgagcataa aagcggcgtg
     1921 gtgatacgta cccgtcaggg cagcgactat gaagcatcga cgctgattag ctgttccggg
     1981 ctgatggctg accggctggt gaaaatgctc ggcctcgaac cgggctttat tatctgcccg
     2041 ttccgcggcg agtatttccg cctggcgccg gagcataacc agattgttaa ccatctgatt
     2101 taccccattc ccgacccggc aatgccgttt ttgggcgtgc atctcacccg catgatcgac
     2161 ggcagcgtca ccgtcgggcc aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag
     2221 cgcgacttct catttagcga cacgctggag attttgggct cgtcggggat tcgccgggtg
     2281 ctgcaaaacc atctacgctc aggactgggc gagatgaaaa actcgctgtg caaaagcggc
     2341 tatctgcggc tggtgcaaaa gtattgtccc cggctttcgt taagcgatct ccagccctgg
     2401 cccgccggtg tgcgggcgca ggcggtatcg ccggacggca agctgattga cgattttctg
     2461 tttgtcacca ccccgcgcac gatccacacc tgcaatgcgc cctccccggc agcgacatca
     2521 gcaattccta ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc aagccagagt
     2581 aaccccggac gcacgctgcg agcggcacgt agtgtggatg ccttacacgc cgcgtttaat
     2641 caataacctt tgaaaacagg atgtagcgat gaaactgaac gacagtaact tattccgcca
     2701 gcaggcgttg attaacgggg aatggctgga cgccaacaat ggtgaagtca tcgacgtcac
     2761 caatccggcg aacggcgaca agctgggtag cgtgccgaaa atgggcgctg atgaaactcg
     2821 cgccgctatc gacgccgcca accgcgccct gcccgtctgg cgtgcgctca ccgccaaaga
     2881 acgcgccaac attctgcgca actggttcaa tttgatgatg gagcatcagg acgatttagc
     2941 gcgcctgatg accctcgaac agggtaaacc gctggctgaa gcgaaaggcg aaatcagcta
     3001 cgccgcctct tttattgagt ggtttgccga agaaggcaaa cgcatttatg gcgacaccat
     3061 tcccggtcat caggccgata agcgcctgat tgtgatcaag cagccgattg gcgttacagc
     3121 ggccatcacg ccgtggaact ttccggcggc aatgattacc cgcaaagccg gtccggcgct
     3181 ggcggcaggc tgcaccatgg tgctgaagcc cgccagtcag acgccgttct ctgcgctggc
     3241 gctggcggag ctggcgatcc gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg
     3301 ttcggcgggc gcggtgggta atgaactgac cagcaacccg ctggtacgca aactgtcgtt
     3361 taccggctcg accgaaattg gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa
     3421 agtgtcgctg gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga
     3481 taaagccgtg gaaggcgcac tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg
     3541 cgccaaccgt ttatacgtgc aggacggcgt gtatgaccgc tttgccgaaa aattgcagca
     3601 ggcggtgagc aaactgcata tcggcgacgg gctggataaa ggcgtcacca tcgggccgct
     3661 gatcgatgaa aaagcggtag caaaagtgga agagcatatt gccgatgcgc tggaaaaagg
     3721 cgcgcgcgta gtttgcggcg gtaaagcaca tgaacgcggc ggcaacttct tccagcccac
     3781 cattctggtg gacgtgccgg ccaacgccaa agtgtcgaaa gaagagacct tcggccccct
     3841 cgccccgctg ttccgtttta aagatgaagc cgatgtgatc gcgcaagcca atgacaccga
     3901 gtttggcctt gccgcctatt tctatgcccg tgatttaagt cgcgtcttcc gcgtgggcga
     3961 ggcgctggag tacggcatcg tcggcatcaa taccgggatt atttccaatg aagtggcccc
     4021 gttcggcggc attaaagcct cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga
     4081 ttacttagaa atcaaatata tgtgcatcgg tctttaactg gagaacgcga atgagcagca
     4141 ataaagagtt aatgcagcgc cgcagtcagg caattcctcg tggcgttggg caaattcacc
     4201 ccattttcgc tgatcgcgcg gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc
     4261 ttgatttcgc gggcggcatt gcggtgctca ataccgggca cctgcatccg aaagtggttg
     4321 ccgcggtgga agcgcagttg aaaaaactgt cgcacacctg cttccaggtg ctggcttacg
     4381 agccgtatct ggagctgtgc gagattatga atcagaaggt gccgggcgat tttgccaaga
     4441 aaacgctgct ggttacgacc ggttccgaag cggtggaaaa cgcggtgaaa atcgcccgcg
     4501 ctgccaccaa acgtagcggc accatcgctt ttagcggcgc gtatcacggg cgcacgcatt
     4561 acacgctggc gctgaccggc aaggtgaatc cgtactctgc gggcatgggc ctgatgccag
     4621 ggcacgttta tcgcgcgctt tatccttgcc cactgcacgg catcagtgaa gatgatgcta
     4681 tcgccagtat ccaccgaatt tttaaaaatg atgctgcgcc ggaagatatc gccgccatcg
     4741 tgattgagcc ggttcagggc gaaggcggtt tctacgccgc gacgcctgcg tttatgcagc
     4801 gtttacgcgc gctgtgtgac gagcacggga tcatgctgat tgccgatgaa gtgcagagcg
     4861 gcgcggggcg taccggcacg ctgtttgcga tggagcaaat gggcgtggca ccagatctca
     4921 ccacctttgc gaaatcgatc gcaggcggct tcccactggc gggcgtcacc gggcgcgccg
     4981 aagtgatgga tgccgtcgct ccaggcgggc tgggtggcac ctatgccggt aatccgattg
     5041 cctgcgtggc ggcgctggaa gtgttgaagg tgttcgagca ggaaaatctg ctgcagaaag
     5101 ccaacgatct ggggcagaag ttgaaagatg gattgttggc gatcgccgaa aaacaccctg
     5161 agatcggcga cgtacgcggg ctgggggcga tgatcgccat cgagctgttt gaagacggcg
     5221 atcacaacaa gccggacgcc aaactcaccg ccgagatcgt ggctcgcgcc cgcgataaag
     5281 gcctgattct tctctcctgc ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca
     5341 ccattgaaga cgctcagatc cgtcagggtc tggagatcat cagccagtgt tttgctgagg
     5401 caaagcagta gcgccgctcc tatgccggag gcgacgctgc gcgtcttgtc cggcctacgg
     5461 ggatccaggt cggataaggc gtttacgccg catccgacaa tctgtacgtg aacaggaata
     5521 aatctatgtt ggccgggtaa ggcggagccg ctctccggca aaaagaatca ataacaatta
     5581 tacgcgtgac ccgacgcggg aaatgtcggg gcgctctccc aagtgacaca ctttcgagag
     5641 gattcaggat ggggcaatca tcgcaaccac atgagttagg cggcgggctg aagtcacgcc
     5701 atgtcaccat gttgtctatt gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg
     5761 tcgccatcgc cgaagcgggc ccggcggtat tactggccta tctgttcgcc gggctactgg
     5821 tggttatgat tatgcggatg ttggcggaaa tggcggttgc cacgcccgat accggttcgt
     5881 tttccaccta tgccgataaa gctattggtc gttgggcggg ttataccatc ggctggttgt
     5941 actggtggtt ttgggtactg gttatcccgc tggaagccaa catcgccgcc atgatcctgc
     6001 actcatgggt tccaggcatt cccatctggt tattttccct cgtcattacc cttgccttaa
     6061 ctggcagtaa tttattaagc gttaaaaact acggcgaatt tgagttctgg ctggcgctgt
     6121 gcaaagtcat cgctatcctg gcctttattt tccttggtgc agtcgcaatt agcggttttt
     6181 acccgtatgc cgacgtgagc gggatctcaa gattgtggga tagcggcggc tttatgccca
     6241 acggcttcgg tgcggtatta agcgcgatgt tgatcaccat gttctcgttt atgggcgcag
     6301 aaattgtcac cattgccgcc gcggaatccg acacgccgga aaaacatatt gtccgcgcta
     6361 ccaactcggt tatctggcgt atttctatct tctatttgtg ctctattttt gtcgtagtgg
     6421 cgttaatacc gtggaatatg ccggggctga aagccgttgg atcttatcgc tcggttctgg
     6481 aattgctcaa tataccccat gcgaaattaa tcatggactg cgtgatatta ctttccgtaa
     6541 ccagttgcct gaactcggcg ctgtataccg cgtcaaggat gctctactcc ttaagccgtc
     6601 gcggtgatgc gcccgcggta atgggcaaaa tcaaccgcag taaaaccccg tatgtggcgg
     6661 tgttactctc caccggagcg gcatttttaa cggtcgtggt gaactattac gcacctgcga
     6721 aagtgtttaa attcctgatc gacagctccg gtgctatcgc cctgctggtt tatttagtca
     6781 tcgccgtttc acagttgcgg atgcgtaaaa ttctgcgagc agaaggaagc gaaattcgct
     6841 tgcgcatgtg gctttacccg tggctcacct ggctggtaat cggctttatt acctttgtgt
     6901 tggtggtgat gttattccgc ccggcgcaac agttagaagt gatctctacc ggcttattag
     6961 cgatagggat tatctgtacc gtgccgatta tggcgcgctg gaaaaagctg gtattgtggc
     7021 aaaaaacacc cgttcataat acgcgctgat aatgatcagg agtcacacca tgaccattac
     7081 gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga
     7141 tgaaaaatta cgaatgagtt tgctgacatc gcgttatgca cttggcgttg ggccgttacg
     7201 ggaagctctt tcgcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta
     7261 tcgggtggcg tctatgtcag agcaggagct gctcgatatt ttcgacgccc gcgccaatat
     7321 ggaagcgatg ttagtgagtc tggcgattgc ccgcggtggc gatgagtggg aggcagacgt
     7381 tctcgcaaaa gcgcatctgc tgagtaagct tgaggcctgt gacgccagcg agaaaatgct
     7441 tgatgagtgg gatctgcgtc atcaggcgtt tcatactgca attgtggcgg gctgtggttc
     7501 tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat
     7561 ctggctgcgg cgaacggtgc tttcggtgga aatgctggag gataaacacg atcagcacca
     7621 gaccctgact gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca
     7681 gcatttactg acgccaattc ccattatcca gcaggcgatg gctggcaatt aactactctt
     7741 ccggaatacg caacacttgc cccggataaa ttttatccgg gctttttagc atcggtttat
     7801 tcgcttcgaa gattttattg tacagattag cgttaccgta gacctgtttg gaaatggcac
     7861 tcagagtgtc gccagactta acggtataaa actggctggc agtggctggt gtcgccgttt
     7921 tcacctgatc atcaacactg gcaataccgg aaatattccc caccgcaaca aggattttct
     7981 ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc tttgccatcg gcaatttgaa
     8041 tgttcacttt atcggcatca ggtataccgg ttttgctcag atgctcctgc accttcttcg
     8101 cctgatcatc tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct ccggcatctt
     8161 tcacaaaatt gaacagaccc atagctacct cattcgttaa cggaaacact cagaaagtgt
     8221 agtagaggaa cgcggcgcgt taacgccgcg gggatgagca caggtttaat cgcgggtttg
     8281 cacccagaac gcgtgaatca aaccggggat atacccaagc agtgtcaaca gaatattaat
     8341 aatgaacgcc caaccgaacc ctttaccgag cagcacgccg agcggcggca gaatgatggt
     8401 gatgacgatt ctccagaaac ccatatgtac tccctataag aaaattactt attgtttaaa
     8461 aagagatttt atctcttaag cgtagtaatt ttagcggagg ctgccagttt cagcttacgc
     8521 ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt tagagtttac
     8581 ttaatttaga aaatacttaa ataactatga ccgaactcac gcaattacag gccagtgctg
     8641 aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg attctgtgca
     8701 tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc ggactgagcg
     8761 cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc aacagccaac
     8821 gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc attatcgcca
     8881 ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc atttcgccgc
     8941 atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt gacgctgatg
     9001 aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg ctggaacagt
     9061 caggtctccc ggctaaatta cgtcgcgatc aaattatatt ccactgccag gcaggtaaac
     9121 gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa atctttttac
     9181 tcgaagatgg gattgatggc tggaaaaaag caggattacc agtagcggta aataaatctc
     9241 aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata ttaatcggcg
     9301 ttatactggg ttataccgta aatagcggtt tcttcttatt aagcggcttt gttggtgccg
     9361 ggttactgtt tgcaggaatt agcggttttt gtgggatggc aaggttgtta gataagatgc
     9421 cgtggaacca atgagcttga gaagcgacgc cggacgcgcc ctagcagcga catccggcct
     9481 cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct gagcgattgg
     9541 cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat cggtgaattt
     9601 atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag cagagctatt
     9661 acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc aggtgctaat
     9721 tttttcctgg cgctctgcca gttcacgctg ctgctgttct tcttcttcac gtctttcttt
     9781 agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga attcgcgagc
     9841 catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca taataaagaa
     9901 aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta
     9961 tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag ctgttatttt
    10021 ctg
//
LOCUS       DAZFTN010000151         9975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFTN010000151 DAZFTN010000000
VERSION     DAZFTN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..142)
                     /locus_tag="ACHKV2_004411"
     CDS             complement(<1..142)
                     /locus_tag="ACHKV2_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942173.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFF"
     gene            220..1314
                     /gene="ddlA"
                     /locus_tag="ACHKV2_004412"
     CDS             220..1314
                     /gene="ddlA"
                     /locus_tag="ACHKV2_004412"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6942174.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1338..1550)
                     /gene="yaiZ"
                     /locus_tag="ACHKV2_004413"
     CDS             complement(1338..1550)
                     /gene="yaiZ"
                     /locus_tag="ACHKV2_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO6942175.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1810..2118
                     /gene="yaiY"
                     /locus_tag="ACHKV2_004414"
     CDS             1810..2118
                     /gene="yaiY"
                     /locus_tag="ACHKV2_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO6942176.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2177..3271)
                     /gene="yaiW"
                     /locus_tag="ACHKV2_004415"
     CDS             complement(2177..3271)
                     /gene="yaiW"
                     /locus_tag="ACHKV2_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO6942177.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3284..4504)
                     /gene="sbmA"
                     /locus_tag="ACHKV2_004416"
     CDS             complement(3284..4504)
                     /gene="sbmA"
                     /locus_tag="ACHKV2_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO6942178.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4856..6013
                     /gene="ampH"
                     /locus_tag="ACHKV2_004417"
     CDS             4856..6013
                     /gene="ampH"
                     /locus_tag="ACHKV2_004417"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO6942179.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6014..6637)
                     /gene="iprA"
                     /locus_tag="ACHKV2_004418"
     CDS             complement(6014..6637)
                     /gene="iprA"
                     /locus_tag="ACHKV2_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO6942180.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6725..9667)
                     /gene="ehaB"
                     /locus_tag="ACHKV2_004419"
     CDS             complement(6725..9667)
                     /gene="ehaB"
                     /locus_tag="ACHKV2_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO6942181.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2596 a         2461 c         2347 g         2571 t
ORIGIN      
        1 aaaaaaactc accgcatggc catttaagcc aggaataaat tagcttcccg ttcatcattt
       61 ttgtgatctt gcgcacatct ttgagataac gcactttctt caccagtacg ttgcacgctt
      121 tgtcagcaga agattgcaac aatctttcag aaggccgcga atcccccgtt ttgtcgtggt
      181 aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga
      241 atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt
      301 gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg
      361 caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt
      421 gcgttgcgcc cttcggcgac cagcctcgcc caggtgccag gtaaacatga gcatcagctt
      481 atcgacgcgc aaaacggtca gccgttgccg acggtggatg tcattttccc gattgtccac
      541 ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt
      601 gtaggttctg atgttctggc ttcagcagcc tgtatggata aagatgtcac caaacgtctg
      661 ctacgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc taatcgtcac
      721 aacatcagtt ttgccgaagt ggagtctaaa ctggggttac cactgtttgt aaaaccggct
      781 aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg aagaacaata cgcaattgcc
      841 gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt
      901 gagatcgaat gcgcagttct gggcaacgac aatccgcaag ccagcacctg tggcgagatc
      961 gtactcacca gcgatttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa
     1021 gtggtagttc cggcagccat tgcgccagaa atcaacgata agatccgggc gattgccgtt
     1081 caggcttatc agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca
     1141 gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg
     1201 tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac acgtttgatt
     1261 gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt
     1321 aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc
     1381 aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa
     1441 ccttttgctt caaatcccag taaccccacc acgccattaa gaatgaatat aaggccaatg
     1501 gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat ggctgctccg
     1561 ttaacgaaaa acaacatcct tgcatattct gcaacaaccg tctgtggtgt agacaacatt
     1621 ttcgcaattg ttaataaatt taacttaaag ttgctatttt gtgactcgca acgaagattc
     1681 acgcttcggg aatattccca acatcaattt accatgattc tgatattgac aatctaaatg
     1741 acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg
     1801 gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat
     1861 gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct
     1921 ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcag
     1981 gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg
     2041 ctgatcccgt ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg
     2101 cgacatcgcc gccagtaaga tttacgttgc cagatacggc ctaaaacgcc ttatctggca
     2161 tgacattcct gtttgttcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg
     2221 caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca
     2281 acatttcgcg ggggagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt
     2341 acagcgccgt ctcctcaaaa gagaagctgt cacctttctc tagctgacga cgaatttcgc
     2401 tgtcgctcat ccccagtttt gccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt
     2461 ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac
     2521 tgaccgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa
     2581 aacggtatat cggtgcgcta taactggcgg gataattcag taaatggtaa gtaccaaacc
     2641 acaacccgcc gcggcgacta aagacttcct gacggactgt accgtccatt ttccacggat
     2701 accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca
     2761 ccggattaag cgaaccaaac aacgtctgcc ccattggcac catgctgatc agatcgtcaa
     2821 aaatcccgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc
     2881 cgtttggcga tttgattttt agcgcagtat gaaccagaaa agcaggaatg tgcatccgtt
     2941 cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg
     3001 cctgataatt tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg
     3061 gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt
     3121 tcacgctcgc aggcatcttc tgccgcacca cgctagccac atctatcgct ttttcgccct
     3181 ttttcaacgg ctgtggcgcc tgactgctac accccgctaa aaccagaacc gccaaaaatg
     3241 aaagcgaaga cagatgattg actcgcgaca ttacttctcc tttttagctc aaggtatggg
     3301 ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga
     3361 tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa
     3421 cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa
     3481 ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcgag
     3541 cgatgttgaa atacatatag tgaaaataga ggcggaaata gtttttccgt acggcgctaa
     3601 acagctcgcg taccgttggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct
     3661 ctttacggta ggcagcctct acacgctggt ttttaaactc cagccccggc agtttgatcc
     3721 ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac
     3781 catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccaaca
     3841 ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacaccc atattctcca
     3901 gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcgcct tcgatatgac
     3961 gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacgt
     4021 agtgactgac aaagaagtta ttcagcacac tgatcactac cgcgatcagc gcaatcccca
     4081 gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg
     4141 cggtttgaat cagatcatag aacggtgcat accaggcgtt gacggcaacc ccaacttcca
     4201 ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag tattgaccag tattgccaac
     4261 ggtgcgggct gtagataaac cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag
     4321 cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg
     4381 aagcgccggt gatacgcgcc acccagtccc ccccaccggc ttgccagaag ataacggcaa
     4441 tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa
     4501 acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga
     4561 ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga ctcatagcaa ggcgcttagt
     4621 tcgccagcct ggccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat
     4681 aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt
     4741 gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat
     4801 caatgagtag tataaatacg ctcagttacc ttcattcaat ttatggacac caccgttgaa
     4861 acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc
     4921 gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg ccgatcatat
     4981 tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt
     5041 ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt
     5101 cgtgcgtatt gcttccctca ccaagctaat gaccagtgag atgctggtga aattgctcga
     5161 ccaggggacc gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt
     5221 gccaacctac aatgggacgc ccattacgct ggtcaatctg gcaacccata ccagcgccct
     5281 gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga
     5341 gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc
     5401 gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc
     5461 ttatacccag ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta
     5521 cactccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg ccagtccgtg
     5581 caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat
     5641 gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc aggcagatcg
     5701 catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca tggatgtccc
     5761 cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc
     5821 ggggattatt cagaagacag gtggtggtgg cggtttcatt acctatatgg cgatgatccc
     5881 gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa
     5941 tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac cgttggttat
     6001 ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat
     6061 tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg
     6121 atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat
     6181 tccagtcaat cattgataat aatgttgcgc ggatttgttc gtaggaatta tgcccaatga
     6241 gctgcacgtc gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc
     6301 gccagagttg attttgttca ataagcgtaa tggtttgttt ggctggtaga tgatatcccg
     6361 tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca
     6421 gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct ctacgcagag
     6481 agataactcc ctctaaaata acgaaagtat cttcgttatt gacatcactg gaaaatataa
     6541 cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct
     6601 taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt gcagtaatgc
     6661 ccgtcaaatc cttgacgggc attatttaga ttaaattacc agtatttctt cggagtgaag
     6721 aatattacca ggtatattta acacccacgt tcgcggacca gtcttgatct acgtcaccac
     6781 caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg aactgagtac
     6841 ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca ccgttcacat
     6901 cgttatcgtt gttagagtcg tcgtagacgt aagccagttt gaagtacgga gtcagagcct
     6961 gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa cgcatgctgt
     7021 cgtaagactg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca ccagactgga
     7081 acagaccaga aatgctgccg taaggagtca cgtaaccagc atcacccagt ttgaagtcgt
     7141 aaccggcttt caaaccgaag ccccaagcgt cggagttggt gctaccgtca acgtaagtac
     7201 cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag ctaccatcaa
     7261 caaagacgtt gttcgcgaag tgagcagaag agtagatgta ggcagtctgg ctgtcttgat
     7321 ccacctgacc agaacggtca ttcatgtcac ctttagcgaa gcctgcagcc gcaccgacga
     7381 tccacttagc gttgttaccg tcaattttgg tatcaacacc gaccatgatg ccgttaacat
     7441 cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt accaccgaag tagcttaccc
     7501 atgcgccgcc gttatcagcc aggccatgac gagagttggt caaacgagta ccaacggtgt
     7561 cttgttccag gttccagata ttggtgttcg cagatgggat gctcagcgcc atgttagcgt
     7621 agtcggtcaa ctccatctgt tgcagaacaa cggtgttacc gcgctgttca gcctgatagg
     7681 tgtatgcacc caggtcagct ttgttagcag cagagaaggt cgcgttgctg tttttgtcgt
     7741 tgacgtagat cagctcgttg ccgttgtagt cagcgataga accagcacca gtagcgttgt
     7801 cgatacgaac tttgtagtta ccggtcgcgg ctgcaacatg gtcaccgttg ttatcaactt
     7861 cgttctgacc agcttcgata gaagcagtgt tgtcgttatc gccgttaccg ttaatagtca
     7921 ggtgaccatc agagttcatt gcgattaagc cataaccgta gtttgacttg gtagtatcgt
     7981 tggtacgatc gttgaccaga tcggtatcca ttacgtaatc actgctatgg atatcgaata
     8041 caccagcgtg aatgttgccg ttagtatcag tggtactggt cagattcagg gtatcggcct
     8101 ggaacgggtt agcatcgaac tgatcaacgt taatatccaa cagaccgtcg ttagttacgg
     8161 tgatggtatt tgcgtacagt gcagcatctt ctaaacccca gcccgcagaa acatcatcag
     8221 acattttaac ggtactattg tcgatggtca gggtattagt cgctacatga ccgtcttcac
     8281 cgatgttcag gttagaaccg ccagtcagag agacagtgtc agagatcaga cgagagtctg
     8341 caacattaac ttgggaaccg ctgttaatac ttaaagtatc aatcagagaa gattttgttg
     8401 tatcccactc tgaaccaccg ttcaaagtca cattaaacaa accgctctgg taaacttcgt
     8461 ttcctacgat atggccattt tcgtcatatt gagtattgat gttatcaaca ccgacagttg
     8521 atgaaggcca caggctgtta gctgcaatat cgagcagagt tgcagttgct tcagtaccaa
     8581 cagcatatcc gtcgtaagaa ccatcaccat cttcatcaac catatgaaca gacattgcag
     8641 cgccaaccca cttgctgccg ttgttcagag tcacatccat acggtctgtg ccatcccaac
     8701 cgttggtatc tacatcacca tcagcatcgc gatagctgtt agcaccttgc gggaagaagt
     8761 tttcatcgaa attactggag aaaacaacat cacccatcag tgttgagttg tctaaagtta
     8821 ctgtagtctg catcgcatta tcagcatacg gatttgcgat tgcggcaatt gcaacatcgt
     8881 ctgcagtcag cgtgttgcta tagttgctgg cattaccagt atggccaaac caaccagtag
     8941 taccttcatc agtccatgaa ccagaggtca cagtagaatc tttaacagta atagtgttat
     9001 taaacacttc gctgctgttt acgccagtgc tgactgtgtt gtcgtcaata tcttcccact
     9061 cataaccctg agtcagcgta atacctgcta catgagagtt attagtgatg gacagatcaa
     9121 cttcctgatc cagtgtaata gcagtaccca tatcgtaaac atctacaaca tgagtgtcag
     9181 cggcattatt ataggtaccg ttgtaagtat aatgctcgta gttgtcatcg atcgttgaat
     9241 tatcaacgct cagtgtcaga cgatcataaa cataaccagt agcacggtca tcagcacaat
     9301 cagtagtcat gcactcagag gtaatcatac cgtgaatagt gctgtttttg attgtcagac
     9361 tattggcgtt ggtattggta gaaataccgt cgtccagata gtaggtagaa atcacgccgt
     9421 taacggtagc gttattaatg accgggtaga tatcgccgtt gtaatagcta tcagcggcat
     9481 agttgttcca gcctacataa ccatcatagt aaacgtcatc agcataggtg gcgtcgttgt
     9541 agtgatggaa agtattgtaa gtttgaccag aaatatcgtt ggtcgctgca ttagcctgag
     9601 aggtgatagc cagagtgcaa gccaatgcta attgtgatac tataagtttc tttttccagg
     9661 agtgcatttg tcatccctcc tcagggacgt aataagttga tccatcaatt atcaatgcat
     9721 agaaaacatc ttatgtttcg cgttgacgat tatgcggcgt tatagaggaa tggttcaatt
     9781 attctttccc ctaagtccga ttgcggacat atataaaaat atctatacgc acaaagaata
     9841 agttgacaaa aacactgcat ttcaacacgt taatgaaatc attaattttg aattatagat
     9901 cctaaaaata ccaaaacaac aaatcagaca aataatttat caataagcaa tgaactttaa
     9961 gtaattcaat ttttg
//
LOCUS       DAZFTN010000152         9721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFTN010000152 DAZFTN010000000
VERSION     DAZFTN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..83
                     /gene="tnaC"
                     /locus_tag="ACHKV2_004420"
     CDS             9..83
                     /gene="tnaC"
                     /locus_tag="ACHKV2_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO6942182.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            304..1719
                     /gene="tnaA"
                     /locus_tag="ACHKV2_004421"
     CDS             304..1719
                     /gene="tnaA"
                     /locus_tag="ACHKV2_004421"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO6942183.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1810..3057
                     /gene="tnaB"
                     /locus_tag="ACHKV2_004422"
     CDS             1810..3057
                     /gene="tnaB"
                     /locus_tag="ACHKV2_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO6942184.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3189..4364
                     /gene="mdtL"
                     /locus_tag="ACHKV2_004423"
     CDS             3189..4364
                     /gene="mdtL"
                     /locus_tag="ACHKV2_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO6942185.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4339..5298
                     /gene="yidZ"
                     /locus_tag="ACHKV2_004424"
     CDS             4339..5298
                     /gene="yidZ"
                     /locus_tag="ACHKV2_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO6942186.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5454..6203
                     /gene="yieE"
                     /locus_tag="ACHKV2_004425"
     CDS             5454..6203
                     /gene="yieE"
                     /locus_tag="ACHKV2_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO6942187.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6225..6791
                     /gene="yieF"
                     /locus_tag="ACHKV2_004426"
                     /gene_synonym="chrR"
     CDS             6225..6791
                     /gene="yieF"
                     /locus_tag="ACHKV2_004426"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO6942188.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6845..8182)
                     /gene="adeP"
                     /locus_tag="ACHKV2_004427"
     CDS             complement(6845..8182)
                     /gene="adeP"
                     /locus_tag="ACHKV2_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO6942189.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8348..9013
                     /gene="yieH"
                     /locus_tag="ACHKV2_004428"
     CDS             8348..9013
                     /gene="yieH"
                     /locus_tag="ACHKV2_004428"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO6942190.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9150..>9721)
                     /locus_tag="ACHKV2_004429"
     CDS             complement(9150..>9721)
                     /locus_tag="ACHKV2_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO6942191.1"
                     /translation="QIKESHKESATNVTFDYKELAPIFEGVEEKQIKALSNQLEHIKS
                     FKTDYDSRLNKYARDFYYFSSAFAINWEDLLKNYQEIRASIKDYDDLLREIKELIISR
                     NKSLEDKRTLFDNKRDNWNSIEVQDEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYK
                     DDKNISDAMRNILDWFERYPDIVQNITQA"
BASE COUNT         2407 a         2396 c         2368 g         2550 t
ORIGIN      
        1 agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaat attgacaaca
       61 agattgtcga tcaccgccct tgatttgccc ttctgtagcc atcaccagag ccaaaccgat
      121 tagattcaat gtgatctatt tgtttgctat atcttaaatt tgccttttgc aaaggccatc
      181 tctcgtttat ttacttgtgt tagtaaatga tggtgcttgc atatatacat ggcgaattaa
      241 tggatattgc agatgcaata ttcacaggga tcactgtaat taaaataaat gaaggattat
      301 gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat tgagccagta
      361 aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat gaacccgttc
      421 ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac cggggcggtg
      481 acgcagagta tgcaggccgc gatgatgcgc ggcgacgaag cttacagcgg cagccgcagc
      541 tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac cattccgact
      601 caccagggcc gtggtgcaga gcaaatctat attccagtac tgattaaaaa gcgcgagcag
      661 gaaaaaggcc tggatcgcag caaaatggtg gcgttctcta actatttctt tgataccacg
      721 cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa agaagccttc
      781 gatacgggcg tgcgttacga ctttaaaggc aactttgacc ttgagggatt agaacgcggt
      841 attgaagaag ttggtccgaa taacgtgccg tatatcgttg caaccatcac cagtaactcc
      901 gcaggcggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa
      961 tatgatattc cggtggtaat ggactccgcg cgctttgctg aaaacgccta tttcatcaag
     1021 cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga aacctacaaa
     1081 tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat gggcggcttg
     1141 ctgtgcatga aagacgacag tttctttgat gtgtacaccg agtgcagaac cctttgcgta
     1201 gttcaggaag gcttcccgac gtatggcggc ctggaaggcg gcgcgatgga gcgtctggcc
     1261 gtaggtctgt atgacggcat gaatctggac tggctggctt atcgtattgc gcaggtgcag
     1321 tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg cggtcacgcg
     1381 gcattcgttg atgccggtaa actgcttccg catatcccgg cagatcagtt cccggcacag
     1441 gcgctggcct gcgagctgta taaagtcgcc ggtatccgcg cggtagaaat tggctctttc
     1501 ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga actgctgcgt
     1561 ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat tgaagccttc
     1621 aaacatgtga aagagaacgc gtcgaatatt aaagggttaa cctttaccta cgaaccaaaa
     1681 gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactact gagtggctat
     1741 aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc ttcctctaaa
     1801 ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg tgttatggtt
     1861 atagcaggta cagtaatcgg tggaggtatg tttgctttac ctgtggatct tgccggtgcc
     1921 tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct tcattccggg
     1981 ttattgttat tagaagcaaa tttaaattat cccgtcggct ccagttttaa caccatcacc
     2041 aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc cttcgttctc
     2101 tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga aacgatatca
     2161 atgaatttgg gttatcacgc taatccacgt attgtcggga tctgcacagc cattttcgtt
     2221 gccagcgtat tgtggataag ctcgttagcc gccagtcgta ttacctcatt gttcctcggg
     2281 ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttcctggt cgattactcc
     2341 attctgcgcg atgccaccag ctccactgcg ggaacgtctt acttcccgta tatctttatg
     2401 gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag cctgattatt
     2461 tgctatggca aacgcaaaga taagctaatc aaaagcgtgg tcttcggttc gctgctggcg
     2521 ctggtgattt atctcttctg gctctattgc accatgggga atattccgcg agaaagcttt
     2581 aaggcgatta tttcctcagg cggcaacgtt gattcactgg tgaaatcgtt cctcggcacc
     2641 aaacagcacg gcattatcga gttttgcctg ctggtgttct ccaacttagc tgtcgccagc
     2701 tcgttttttg gtgtcacgct gggattgttc gattatctgg cggacctgtt taagattgat
     2761 aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc cgcgttgttg
     2821 tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccggact gtgcgccact
     2881 atttgggcgg tcattattcc cgcagtgctg gcaatcaaag ctcgcaaaaa gtttcccaat
     2941 cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct ctttggtata
     3001 accgtaattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc
     3061 cttcaagaag ccagtcattc gctggcttct tgcctctcag gaaatcactt atgtccaaat
     3121 ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact atcaggacgc
     3181 tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt tatcccgctg
     3241 ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag
     3301 cgcagttaca tattgcgttc tccgtttatc tggcaggtat ggcagctgcg atgttatttg
     3361 ccggtaaagt ggccgatcgt tcagggagaa aaccggtcgc catccccggc gcggcgctat
     3421 ttattatcac ttcggtgttc tgttcactgg ctgaaaccag cacgttattt cttgcagggc
     3481 gctttctaca ggggttgggc gcgggctgtt gttacgtggt ggcattcgcc attttgcgcg
     3541 acacgctgga tgatcgacgt cgggcaaaag tgctgtcgtt actcaacggc attacctgca
     3601 tcattccggt gttagcgccg gtgctcggtc atctgattat gcttaaattc ccgtggcaga
     3661 gtctgttctg gacgatggca ataatgggca tcgcggtact gatgttgtct ttgtttattt
     3721 taaaagaaac gcgccctgcc gcccccgcag cttcggacaa atcacgagaa aatagcgagt
     3781 cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc gtttcggtga
     3841 tcctcacctt cgtcaataca tcgccggtat tgctgatgga aatcatgggt tttgagcgcg
     3901 gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt tcattttcca
     3961 cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctca caggtgttat
     4021 tcctggcggc gggcatcact cttaccgttt caccttccca tgcggtttct ctgtttggta
     4081 tcacgctgat ttgcgccggt ttctcggtag gttttggtgt ggcgatgagt caggcgttag
     4141 ggccgttttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg caggtttgcg
     4201 gttcgtcact gtggatttgg ctggcagcgg tggttggtat cagcgcatgg aatatgctga
     4261 tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc gtcgcgcctg
     4321 gacgcccggt taccgctcat gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc
     4381 tgtctgcagc tgctgatgca ggagcgcagc gtaaccaaag cggcgaagcg gatgaacgtg
     4441 acaccttcgg cggtgagtaa gtcgctggca aagttaagag cgtggtttga cgacccgctc
     4501 tttgtgaact caccgttggg gctgtcgccc acaccgctaa tggtcagcat ggagcaaaat
     4561 ctggcggagt ggatgcaaat gagcaacctg ctgctggata aaccgcacca ccagacaccg
     4621 cgcggcctga agtttgagct ggcggcagaa tcaccgctaa tgatgatcat gcttaatgcg
     4681 ctgtcgaaac agatctacca acgttacccg caggcgacca tcaaattacg taactgggat
     4741 tacgattcct tagatgccat tactcgtggt gaagtggata tcggtttttc cggtcgcgaa
     4801 agtcatcccc gttcgcggga gctgttaagc tcgctaccgt tagccattga ttatgaagtg
     4861 ttgtttagtg atgtgccctg cgtctggtta cgcaaagatc atccggcact gcatgaaaag
     4921 tgggatctcg ataccttttt acgttatccg catatcagca tttgctggga acagagcgat
     4981 acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgtacgat tgcaatgagc
     5041 ctgccggaat tcgagcagtc actgtttatg gcagcgcagc ccgacaatct gctcctggcg
     5101 accgcgccgc gctactgtca gtactacaat caactccacc aactgccgtt ggttgctctt
     5161 cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag tcccttttac cctgctgtgg
     5221 cataaacgga acagccataa tccgaagatc gtctggttac gggataccat taaaaatctt
     5281 tacgcgtcga tggcataacc gaatctatga aacgcgaccc gatttcacaa taaaatgtaa
     5341 aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg tccatcattc
     5401 cagtaaatga ttactcttgt attcataatg gaccattaag catggagcga aaaatggcga
     5461 ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt ctcccctccc
     5521 agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt ctggcgtcca
     5581 gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg ccggaaatcg
     5641 tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg ttttctattt
     5701 cctatgccgg gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt ggcctcgata
     5761 tggaactgca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat aaccacacat
     5821 tttccagcaa tgaatcgcta tgggtcagta aacaaaatga tccaaacgaa gcgcgggcgc
     5881 agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg aatgacgatc
     5941 cgcgcgatct gcaactgctg cccattgccg gacgcctgaa atgtgctcat gtaaatcatg
     6001 tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc acgcccacga
     6061 ttgaaaagct cagtgtctgg gagttagatg gcaaacacgg ctggaaaagc ctgccggata
     6121 ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag ctctctacca
     6181 tgaaggcttt ttcgccaaat tgatagacaa ccacaggagt cattatgtct gaaaaattgc
     6241 aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg gttgcacgta
     6301 ccctaccgaa aattgctccg gcgagcatgg aagtcaatgc gttaccttcc attgccgaca
     6361 ttcccctgta tgacgctgac gtacagcagg aagaaggttt tccagcaacg gttgaagctc
     6421 tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa tataactatt
     6481 cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg gatcaaccgc
     6541 tggccggtaa accggtgttg attcagacca gctcaatggg cgtgattggc ggcgcgcgct
     6601 gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg aacaagccgg
     6661 aatttatggg cggcgtgatt cagaacaaag ttgatccgca aaccggagaa gtgattgatc
     6721 agggtacgct ggaccacctg accgggcaat tgaccgcatt tggcgagttt attcagcgag
     6781 ttaagatcta aataaaaaac ccgccagcaa tcatgcatgg cgggttttta acgcgctatc
     6841 gattttagtg agcgtcgata aatacaatct tcaggataaa cagcagcgca acgatgatta
     6901 cgcacgggct aaggtcacga atacgcccgg taccaatctt catcacgcag taggagataa
     6961 agcccagcgc aataccttcg gtaatcgaga agctgaacgg catcatcaca gcggtaataa
     7021 acgccggaac agattcagta agatcctgcc agttcacgcg agcaagactt gaggtcatca
     7081 acacgccaac gtaaatcagc gcgccagctg cagcgtagcc tggcaccatt cccgccagcg
     7141 gcgacagaaa gataaccagc aggaacagca gaccaacaac cactgccgtc agaccggtac
     7201 gaccgccaac cgatacgccg gaagaggact caatataagc cgtaacggaa gaagtaccga
     7261 taaacgaacc ggtcacggaa gagatactgt cgacatacag cgcctgcttc atgcgcggga
     7321 atttcccctt ctcatctgcc agaccggctt tatcggtcac gccaatcaac gtgccggagg
     7381 agtcaaacag gttgaccagc atgaaagaga aaatcacccc tgccagcccg aggttaaacg
     7441 acccggctaa atctacatga cccacgacag tcattacgct cggcggcgca gaaacgatgc
     7501 cattgtagtg gacatcaccc agcatccagc ccagcagcgt tgtcaccacg atagaaacca
     7561 gcaccgctgc gtgaatgttg cgtgaggcca gaatagcaat gatgaagaag ccgaggatcc
     7621 ccagcagcac gctgtgagaa gtcagattac cgatgctgac cagcgtttcc gggttagcga
     7681 caatcacacc tgcgtttttc agccccatca tgccaatgaa cagaccgata ccgctggtaa
     7741 tgcccacacg cagactcacc ggaatgttgg caatcatcca gtagcgaacg cggaaaatcg
     7801 tcagtaacag cagaccaatc gcgccccaga agattgcgcc catcccgact tgccacggca
     7861 agcccatcgc ctgcacaacg acaaaagcga agaacgcatt caggcccata gcgggtgcca
     7921 gtgcaactgg caggttagca aacagcccca tcataatact gccgaatgca gcaatcagac
     7981 aggtagtgac gaagacggcg ctggtatcca tgccagcaac gccaagaatt tgcgggttaa
     8041 caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact tcggtccgtg
     8101 ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg ccagaagcct
     8161 gggttgtgtg ttgttgactc attatctatt tccgaacaag gagggaaaat ccgtcgctat
     8221 cgtataccaa aatgagacaa tatggacggc tgaagataac ttttttcatt gtatttgctt
     8281 atacggcaac gattgcgtca tgcgaattgg catgacgaaa acgttaaact aacgataaag
     8341 gaaagacatg tcccagatag aagcggtatt tttcgactgc gacggtacgc tggtcgatag
     8401 cgaggtcatt tgctctcgcg cctatgtcac gatgttcagg gaatttggca ttcacgttga
     8461 cctgaaagag atttttaccc gttttaaagg cgtaaaactc tacgaaatca tcgatatcat
     8521 cagtaaagaa cagggcgtga cgatggtcca ggccgatgcc gagcacgttt accgcgcaga
     8581 agtagcacgt ctgtttgata ccgaacttga ggacatcgca ggagcgaacg aattgctggc
     8641 gagcattaac gtgccgatgt gcgtcgtctc taacggtccg gtcagcaaaa tgcagcattc
     8701 gcttggtaag ctcaacatgc tgcactactt cccggaaaaa ctgttcagcg gatatgacat
     8761 tcagcgttgg aagcccgatc cggcattaat gttccatgcg gcaaaagcga tgaatgtgaa
     8821 cgttgaaaac tgcattctgg tcgatgactc cagcgctggc gctcaatcag ggatagatgc
     8881 cggaatggaa gtgttttact tctgcgctga cccgcacaat aagccgatcg ttcacccgaa
     8941 agtcaccacc tttaccgagc tgtcgcagct gcctgcgctc tggaaggcac gcggctggaa
     9001 tataacgcga taatcgcctc cttacttcaa cacttacaca tcagtcacgc tggtttgaga
     9061 tacagaacac caggtgtctg atgagtaagc gtctattccc tatgaataat ttatgtcgtt
     9121 attcgtcctg tcatccaccc tctgtatggt taagcctggg ttatattttg tacaatatcg
     9181 ggataccgtt cgaaccaatc cagaatatta cgcatcgcgt cactgatgtt tttatcgtct
     9241 ttatattgat tctcaagtct attatttcga acaatagtaa gcttagaacg tattttatca
     9301 tcacaatcga caattttcgc attcaatgca tttacttcat cctgcacttc tatactattc
     9361 caattgtccc ttttgttatc aaataaagtc cttttatcct ctaatgattt attacgactt
     9421 atgattaact ctttaatttc tcgcaataag tcatcgtaat ctttaatgga agctcttatt
     9481 tcctgatagt tttttagtaa atcctcccag ttaattgcaa atgcactgga aaaatagtaa
     9541 aaatcgcgag catacttatt gagccttgag tcgtaatctg tcttaaacga ttttatatgt
     9601 tccagttggt ttgatagtgc tttaatttgt ttttcttcaa caccttcaaa aatgggtgca
     9661 agttccttat aatcaaatgt tacatttgta gcagattctt tatgagactc ctttatttgc
     9721 t
//
LOCUS       DAZFTN010000153         9695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFTN010000153 DAZFTN010000000
VERSION     DAZFTN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            198..617
                     /locus_tag="ACHKV2_004430"
     CDS             198..617
                     /locus_tag="ACHKV2_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6942192.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            617..1885
                     /gene="umuC"
                     /locus_tag="ACHKV2_004431"
     CDS             617..1885
                     /gene="umuC"
                     /locus_tag="ACHKV2_004431"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO6942193.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1931..2461)
                     /gene="dsbB"
                     /locus_tag="ACHKV2_004432"
     CDS             complement(1931..2461)
                     /gene="dsbB"
                     /locus_tag="ACHKV2_004432"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO6942194.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2607..4148)
                     /gene="nhaB"
                     /locus_tag="ACHKV2_004433"
     CDS             complement(2607..4148)
                     /gene="nhaB"
                     /locus_tag="ACHKV2_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO6942195.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVALG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4370..5089
                     /gene="fadR"
                     /locus_tag="ACHKV2_004434"
     CDS             4370..5089
                     /gene="fadR"
                     /locus_tag="ACHKV2_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO6942196.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5141..6673)
                     /locus_tag="ACHKV2_004435"
     CDS             complement(5141..6673)
                     /locus_tag="ACHKV2_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO6942197.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7036..8334
                     /gene="dadA"
                     /locus_tag="ACHKV2_004436"
     CDS             7036..8334
                     /gene="dadA"
                     /locus_tag="ACHKV2_004436"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO6942198.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8344..9414
                     /gene="dadX"
                     /locus_tag="ACHKV2_004437"
     CDS             8344..9414
                     /gene="dadX"
                     /locus_tag="ACHKV2_004437"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO6942199.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT         2412 a         2392 c         2601 g         2290 t
ORIGIN      
        1 tctatttttt agccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
       61 ccgcgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
      121 tagcctgaat cagtattgat ctgctggcaa gaacagacta ctgtatataa aaacagtata
      181 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
      241 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggccgca gattacgttg
      301 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
      361 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
      421 atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
      481 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agcgcgtact
      541 cgcccattac catcagtagt gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
      601 ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga
      661 gacggtgttt cgccctgagt tatggggtaa accggtggtt gtgctatcga ataatgacgg
      721 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgcttggc gttaaaatgg gcgatccctg
      781 gttcaaacaa aaagatctgt ttcgtcgctg tggcgtggtt tgctttagca gcaattatga
      841 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
      901 cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga
      961 tctgactgac tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     1021 tggtgtgggg atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa
     1081 atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt
     1141 aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca gcaaaaaact
     1201 ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc ggtttatccg
     1261 taaacatttt aatgtcgtgc tcgaaagaac ggtgcgtgaa ctgcgcggtg aaccctgttt
     1321 gcaactggaa gaatttgcac cgacaaagca ggaaattatc tgttcccgct cgtttggtga
     1381 acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg cccgggcggc
     1441 ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta ttaagacgtc
     1501 accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac tgctgacgcc
     1561 cactcaggac agcagggata tcattaacgc cgctacgcga tctctggatg ccatctggca
     1621 agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt
     1681 cgcgcagctc aatttattcg atgacaacgc tccgcgcccc gggagtgagc aattgatggc
     1741 ggtaatggat acactgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg
     1801 gatccagcaa caatggcaga tgaagcgagc catgctttca ccacgttata caacgcgaag
     1861 ttctgattta ctgcgggtca aataaacaca gcggcaggaa aaaagcgctc ccgcaggagc
     1921 gccgaatgga ttagcgaccg aacaggtcac gttttttcgc tttaaacggc tgggaaatca
     1981 ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac tgcggcattt
     2041 ccagacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca aacacttgcg
     2101 gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca caggtggcaa
     2161 acggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca ccgcggaacg
     2221 cactatacaa ccagataacc atcgctacat aacgcagtgg cgtttttggg gcgatcgcgc
     2281 caatcagcgc agcacccaga acgccgaata atgcgcagcg ttcataaata cagagcacgc
     2341 aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt gccagagcag
     2401 taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa aatcgtaaca
     2461 taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat tcgctgcgcc
     2521 accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa ggcaaaccgg
     2581 gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat ttgcataaac
     2641 cattcggtta caggggcaag cgtaaactcg acgcagagca gtccgacgag tgtcaggacg
     2701 agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat caatggcgcg
     2761 agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc gacagagggc
     2821 agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa cgtgatagcg
     2881 ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa gacgttatcc
     2941 gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc atgttccgat
     3001 gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat caccgcgact
     3061 accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa ggctttaccg
     3121 atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac tgacaaacca
     3181 atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac gccaataatc
     3241 gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct ttgatcgtca
     3301 aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc aaaccaacgc
     3361 agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac ggtcaccggc
     3421 gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat gatcaggttc
     3481 tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc ggcatgcatc
     3541 atcaggctac gcagaaagcc acggaactgt tccagaacca ctttgtaatg cttgtcgata
     3601 tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg atgataaata
     3661 ccataaaaac cgagggcaac gctgatcacc accgccacga cggttaaggc atcgaggaac
     3721 gcggagagga acgctgccgc tacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg
     3781 cttaacagca aacgggtaaa tatgaacagc aatagctgtt tcataaaata gatacccgcc
     3841 accataaaca tcagtaacag caagacttca agatttgccg ccacctcttc acggacgtgt
     3901 tccgcgctgg tcatgccgat gaataccgct tcgatagcca acaggccacc ggggagcagc
     3961 gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag caaccagcca
     4021 gcgacgaaag ggctgatgag gaaaattaac ggatttacga ttaagaaaat gatgagggcg
     4081 agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg gccccaggag
     4141 atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat gtttaaacgc
     4201 aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga aagagatgcg
     4261 gaaaatgaag ccttgatccc ttttttcttc tttttgtctg ctatcagcgt agttagccct
     4321 ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta tggtcattaa
     4381 ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct ggaataaccg
     4441 cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa ttggcgtaac
     4501 gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt tgaccattca
     4561 acatggcaag ccgacgaagg tgaataattt ctgggaaacc tctggtttaa atatccttga
     4621 aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt tgctgtcggt
     4681 gcgtaccaat atttcaacta tttttattcg caccgcgttt cgtcagcatc ccgataaagc
     4741 gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct ttgccgagct
     4801 ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt acggtctgat
     4861 ccttaacggg atgaagggac tgtatacgcg cattggtcgt cactatttcg ccaatccgga
     4921 agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca gtgaaggcgc
     4981 gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg agatttggca
     5041 ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat cccttccgtt
     5101 taaaaaacaa acccctcaaa cgaggggttt tttgttgttt ttacagattt cccattctcg
     5161 gcgggcaacg ttccagcaac tcgacgctgc cgtcttcgtt ttgctgttcc agcatcacat
     5221 caaatcccca caggcgatgc acatgcttca gtacttcttt gcgcccccga tccagcggtg
     5281 cgcgattatg tggaatgtaa cgcagcgtca gcgaacggtc gccgcgcaaa tccacgttcc
     5341 agatctgaat attcggctcc agattactta agttatattg cgacgatagc cggttacgga
     5401 tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta tgccgatcat
     5461 catccagcac ggtgaagaag cggaaatcac gcatcacttt cggcgacagg aactggctga
     5521 taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc cagtcggaac
     5581 cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa atccgtttaa
     5641 tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac cacgggctgt
     5701 tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc ataaaacgtt
     5761 ccgttacttt cccttcatca tacagatggt taaggatggt gtagtgccag aaggtggccc
     5821 agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc accttacgca
     5881 caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc tccataaaat
     5941 agagcaggtt ttcttgtggt tcggacggat aacggcgcgc ttcagcaacc gttttctctt
     6001 cctcgcgctt cggcagggta cgccagagca tattgacctg actttgcaaa tactcttcgc
     6061 gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt ttgtaccggt
     6121 ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact tcatcaacgc
     6181 cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa tcgacaatcg
     6241 aactggcgtc ggtccagcta cggaataagt aattgttttt gaagaaagag ttatgcccat
     6301 agcaggcatg agccatcacc agcgcttgca tggtaatggt gttctcttcc atcaggtaag
     6361 cgatacacgg gttagagtta atgacgattt cataggccag tccttgctga ccgtgcttat
     6421 acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag ttaattggca
     6481 tgccgacgct ggagtaggca tccatcatct gttctgaggt tatcacttca atctggtgcg
     6541 ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga taaacatcca
     6601 gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta ttcatagaat
     6661 cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga gcacctcatt
     6721 gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc
     6781 gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgtt
     6841 tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca
     6901 tattgccgca gagtcaggga gatgtgagcc agctcaccat aaaaaagccg tatgttgaat
     6961 aatattttca actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc
     7021 gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc
     7081 gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc
     7141 gccctggaaa ccagtgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg
     7201 gcggcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgccgctg
     7261 gcggttcgtc tcgacggtac gcagttccag ttgaaatgga tgtggcaaat gttacgtaac
     7321 tgcgacacca gccactacat ggaaaacaaa gggcggatgg tgcgtctggc ggaatacagc
     7381 cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt
     7441 gggacgctgc aactgttccg taccgaacaa cagtatgaaa atgcgacccg cgatatcgcc
     7501 gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg
     7561 gagcccgcgc tggcagaagt agcgcacaaa ctgacgggtg gtctgcaatt gcccaatgat
     7621 gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcgga gcaggcgggg
     7681 gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac
     7741 ggcgtgaagt gtggcgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct
     7801 tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac
     7861 tcgctgacca ttccaattgc gcaggaagat ggtgcgccgg tatccaccat tcttgatgaa
     7921 acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgttggcgg aatggcggaa
     7981 attgttggtt ttaataccga gctgttgcaa ccgcgtcgtg aaacgctgga gatggtggtt
     8041 cgcgatctct atccacgcgg cggtcatgtc gagcaggcga ctttctggac gggtctgcgc
     8101 ccgatgacgc cagacggcac gccggttgtc gggcgtacac gctttaaaaa tctgtggcta
     8161 aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc
     8221 gatctgctct ctggtcgcac gccagcgatc ccatatgagg atctaagcgt agcgcgctac
     8281 agccgtggat ttacgccatc acgtccgggc catttacatg gcgcacacag ctaaggaaac
     8341 gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc
     8401 attgtccgcc aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc gaacgcttac
     8461 gggcacggta ttgagcgtat ctggagcgcg ctcggggcta ccgatggctt tgcattactt
     8521 aacctggaag aagcaataac gttacgtgag cgcggctgga aaggaccgat cctgatgctg
     8581 gaaggatttt tccatgctca ggatctggaa atgtatgacc agcaccgcct gaccacctgc
     8641 gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat
     8701 atttatctta aagtgaacag tgggatgaat cggttgggct tccagcccga tcgcgtgctt
     8761 accgtctggc agcagttgcg ggcgatggcg aatgttggcg aaatgaccct gatgtcgcat
     8821 tttgccgagg cggaacatcc tgatggaatt tccggcgcga tggcgcgtat tgagcaggcg
     8881 gcggaagggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcaccca
     8941 gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt
     9001 cagtggcgtg atatcgccaa tactggatta cgtccggtga tgacgctaag cagtgagatt
     9061 attggtgtcc agacgctaaa agcgggcgag cgtgtgggct acggcggtcg ttatactgcg
     9121 cgcgatgagc agcgaatcgg cattgttgcc gcaggatacg ccgacggtta tccgcgccac
     9181 gcgcctaccg gtacccctgt tttagtggac ggcgtgcgca ccatgacggt ggggacagtc
     9241 tcgatggata tgctggcggt cgatttaacg ccttgcccgc aggcgggtat tggtacgcca
     9301 gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg
     9361 ggctatgagt tgatgtgcgc gctggcgtta cgcgtcccgg ttgtgacggt gtaacttgtt
     9421 gtaagtcgga tcggaggcaa cgtcttctgg ttgcaaaaaa aatcatccat ccggctggtc
     9481 agcaactgta gttgttaatg tgacagagcc attgcccatg atagtgccca ttaaaaggat
     9541 ggacactatt tccccggaac ctgaactcac cgcacaggcg ttctacataa aacgcttacg
     9601 cttcattgtt gactcgaact cgacttcaga taaatcacgc ttcacccttg atgcagattc
     9661 gggttcatgt tcagaatgtt gattaacagg ctgcg
//
LOCUS       DAZFTN010000154         9577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFTN010000154 DAZFTN010000000
VERSION     DAZFTN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..253
                     /locus_tag="ACHKV2_004438"
     CDS             <1..253
                     /locus_tag="ACHKV2_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6942200.1"
                     /translation="KNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDL
                     TPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            225..2154
                     /locus_tag="ACHKV2_004439"
                     /pseudo
     CDS             225..2154
                     /locus_tag="ACHKV2_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2138..2344
                     /locus_tag="ACHKV2_004440"
     CDS             2138..2344
                     /locus_tag="ACHKV2_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO6942201.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2341..3933
                     /locus_tag="ACHKV2_004441"
     CDS             2341..3933
                     /locus_tag="ACHKV2_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6942202.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3923..5428
                     /locus_tag="ACHKV2_004442"
     CDS             3923..5428
                     /locus_tag="ACHKV2_004442"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6942203.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5507..5812
                     /locus_tag="ACHKV2_004443"
     CDS             5507..5812
                     /locus_tag="ACHKV2_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6942204.1"
                     /translation="MFAGNDPAHTATGSSGISSATPALTPLMLDEASGKLVVWDGQKA
                     GSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKANAFAGSALSHAALP"
     gene            5870..6121
                     /locus_tag="ACHKV2_004444"
                     /pseudo
     CDS             5870..6121
                     /locus_tag="ACHKV2_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6118..6858
                     /locus_tag="ACHKV2_004445"
     CDS             6118..6858
                     /locus_tag="ACHKV2_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942205.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6872..7303
                     /locus_tag="ACHKV2_004446"
     CDS             6872..7303
                     /locus_tag="ACHKV2_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6942206.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7330..7743
                     /locus_tag="ACHKV2_004447"
     CDS             7330..7743
                     /locus_tag="ACHKV2_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6942207.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7724..>9577
                     /locus_tag="ACHKV2_004448"
     CDS             7724..>9577
                     /locus_tag="ACHKV2_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6942208.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQ
                     QAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAALAKAT"
BASE COUNT         2215 a         2343 c         3032 g         1987 t
ORIGIN      
        1 aaaaaatgag gtgtcaaaag gaaaactgat tgacaccggg ttctgtattt ttgccctcag
       61 taagctggca atggcgttat ccagtacgct tgattccatc cctttatcca tgcagcgaca
      121 gtttcctgat ttaacaccgc gccatcttga ccatctgaaa acccttattg ctaagggggc
      181 aaatcagtgt gcgcgggcag gggataaatt accggattta cttgatgaat atatcagagc
      241 aacaactgaa taatatgatg agcgctgtca caactgcatt acagcccctg ataagggcat
      301 tgccggtgac gccagttgaa tgggctgatc aaaattatta tctgcctaaa gaatcttcat
      361 atggtgaggg cgaatggaaa acgctgccat tccagatcgc catcatgaac agtatgggga
      421 atgatcagat ccgcactgtt aatctgatta aatctgcccg tgttggctat acaaagatgt
      481 tgctgggggg ggggcgggta ttttattgag cataaatccc gaaacagtct gctttttcag
      541 cccacggatt ctgccgctga agattttatg aagtctcacg tggaggcgac gattcggaac
      601 gtgccatgcc tgaaagacct ttccccatgg ctgggtcgta aacatcgtga caatactctc
      661 acgctgaaac gcttttcatc gggcgtcggt ttctggtgcc tgggcggcgc tgccgccaaa
      721 aactaccgtg aaaaatccgt tgacgtggtc tgctatgacg aactttcctc gttcgagccg
      781 gatgtcgaaa aagagggctc gccaaccctg ctgggggata agcgtattga ggggtcggtg
      841 tggccaaaat ccattcgcgg ctcgacgcct aaaatcaaag gcacctgcca gatcgaaaaa
      901 gccgctaacg agtcggcgca ttttatgcgt ttttatgtgc cctgcccgca ctgtggggag
      961 gcgcagtatc tgaaatttgg cgatgagtcc acgccttttg ggcttaaatg ggagaaggac
     1021 agccctgaaa gtgttttcta cctctgtgaa catcatggct gcgtgatcca tcagtctgaa
     1081 ctggaccaga gcaacgggcg gtggatctgt gaaaacacgg gcatgtggac ccgtgacggt
     1141 ctgacgtttt tcagcgcccg gggtgatgaa attccgccgc cgcgctccat catgttccat
     1201 atctggacgg cgtacagtcc gttcaccacc tgggtacaga ttgtctatga ctggctggat
     1261 gcactgaaag atcccaacgg cctgaaaacc tttgtgaaca ccacgctggg cgagacctgg
     1321 gaagaggccg tgggcgaaaa actcgatcac caggtactga tggataaggt ggtgcgttac
     1381 acggcggcgg tgcctgcccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac
     1441 cgttttgaga tgtatgtctg gggatgggct ccgggagagg aagcctttct ggtggataaa
     1501 atcatcatta tggggcgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc
     1561 aacaaaaaat accgccatgc ggatggcacc gaaatgacta tttcccgtgt ctgctgggac
     1621 accgggggga tcgatggtga aattgtttat cagagatcaa aaaaacacgg tgttttccgg
     1681 gtgctgccgg taaaaggcgc atctgtctat ggcaagccgg tgatcaccat gccaaaaacc
     1741 cgcaatcagc ggggcgtgta tctgtgtgaa gtgggaacgg acaccgcaaa agaaattctc
     1801 tatgcccgta tgaaagccga tccctcgcct gcggatgaag ccacgtcgta tgccatccgt
     1861 tttcctgatg atccggagat tttttcgcag acagaggcgc agcaactggt ggcggaagag
     1921 ctggtggaga agtgggaaaa aggaaagatg cgtctgctgt gggataacaa aaagcggcgt
     1981 aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat tacgtgtgtc cgtgcaacgc
     2041 tggcagcttg atctggctgt actggcaaaa tcccgggaag aagagacgac ccggccaacc
     2101 ctgaaagaac tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca
     2161 ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa cgggtggtgt ctgtccagaa
     2221 agatggtcgc agaattgaat atacggcggc ttctctggat gagcttaacc gggcgatcaa
     2281 tgatgcggag tcggtactgg ggacaacccg gcgtcgccgt cgtccgctgg gagtgaggtt
     2341 atgaaacgaa cgcctgtcct gattgatgtg aacggcgttc cgcttcgtga gagtctcagc
     2401 tacaacgggg gcggtgcagg atttggcggg caaatggcgg agtggttgcc accggcgcag
     2461 agtgccgatg cggccctgct gcccgcgttg cgtctgggga atgcccgggc agatgatctg
     2521 gtgcgcaata acggaatagc ggccaatgcg gtggcactgc ataaggatca cattgtcggg
     2581 catatgtttc ttatcagcta ccgtccgaac tggcgctggc tggggatgcg ggagaccgca
     2641 gcaaaaagct ttgtcgatga ggtggaggcg gcctggtcgg aatacgccga agggatgtct
     2701 ggcgagatcg acgtggaagg aaaacgcacg ttcacggaat ttatccgtga aggtgtgggc
     2761 gttcatgcgt ttaacggcga aatctttgtg cagccggtct gggatacgga aaccacgcag
     2821 ttattccgta cgcgttttaa agccgtgagt ccgaaacggg tggacacgcc aggacacggt
     2881 atggggaacc gttttctgcg ggccggggtg gaggtcgatc gatatggccg tgccgttgcg
     2941 taccatatct gtgaggatga ttttcctcgc tccgggagtg gacgatggga acggatcccg
     3001 cgtgaacttc ccaccgggcg tccggccatg ctgcatattt tcgagccggt ggaggacggg
     3061 cagacccgtg gggccaacca gttttacagc gtcatggaac ggctgaagat gctcgattcc
     3121 ctgcaggcaa cacagcttca gtcggccatt gtgaaagcca tgtatgcagc gacgattgaa
     3181 agtgaccttg ataccgaaaa ggcctttgaa tatatcgccg gtgcgccgca ggggcagaag
     3241 gataatccgc ttattaatat tctggagaag ttctccagct ggtatgacac gaataacgtg
     3301 acgctgggtg gtgtcaaaat tccgcacctt ttccccgggg atgatctgaa actacagact
     3361 gcgcaggatt cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatatcgcc
     3421 gccggtcttg gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttattca
     3481 agtgccaggg cctctgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt
     3541 gcggcccggc tggccacgca gatgttttcc tactggctgg aagaggcact tcttcggggg
     3601 attatccgtc cgccacgggc gcgttttgat ttttatcagg cgcgatcagc ctggtcacgg
     3661 gcagagtgga ttggtgccgg aagaatggcc attgacgggc tcaaggaggt tcaggaatcg
     3721 gtgatgcgca ttgaggccgg actgagcacg tatgagaaag agccggcgct gatgggcgag
     3781 gattatcagg acattttccg ccagcaggtc agggaatctg cagagcggca aaaagccgga
     3841 ctctcacgtc cggtgtggat agcgcaggcg tatcagcagc agatagcgga gagtcgcagg
     3901 ccggaagagg agacaacacc ccgtgagacg taatctttca cacattattg ccgcagcatt
     3961 caatgaaccg ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg
     4021 cgagatgggg gcagcaagtc tttcggtacc acagcagcag gtacagcttg atgctcccgg
     4081 aatgctggct gaaacggacg agtacatggc cggaggtaaa cgaccggccc gtgtttaccg
     4141 ggtggtgaac ggtattgctg tactgccggt gaccggcacg ctggtgcacc ggctgggggg
     4201 tatgcggcca ttttccggaa tgacaggcta tgacggcatt gtcgcctgtc ttcagcaggc
     4261 aatggcggat agccaggtgc ggggcgtact gctggacatt gacagtccgg gcgggcaggc
     4321 cgccggcgcg tttgactgcg ctgacatgat ttaccgcctc cgtcagcaga agccggtctg
     4381 ggcactgtgc aatgacacgg cctgttctgc agccatgctg ctggcgtcgg cctgctcccg
     4441 acggctggtt acccagacat cccgtatcgg ctccattggc gtgatgatga gccatgtcag
     4501 ctatgccggt catctggcgc aggccggtgt ggatatcacg ctgatttact caggggcgca
     4561 caaggtggat ggcaatcagt ttgaagcctt accggcagag gttcgccaga acatgcagca
     4621 gcgcattgat gcggcgcgcc ggatgtttgc cgaaaaagtg gccatgttta ccggtctgtc
     4681 tgttgatgcc gtcacgggaa cagaggccgc cgtttttgaa ggtcagtccg gcattgatgc
     4741 cgggctggcg gatgaattag tcaatgcgtc ggatgccatc agtgtgatgg ccacggcgct
     4801 gaacagtaat gtcagaggag gcactatgcc gcaattaact gcaacggaag ccgccgcgca
     4861 ggagaaccag cgagtgatgg ggatcctgac atgccaggaa gcgaaaggac gtgaacagct
     4921 tgccacgatg ctggcaggac aacagggcat gagcgttgaa caggcccggg cgattctggc
     4981 cgcggcggca ccgcagcagc cggtggcatc cacgcagagt gaagccgatc gcattatggc
     5041 gtgtgaagaa gcgaacggtc gtgaacaact ggcggcaacg ctggcggcga tgccggagat
     5101 gacggtggaa aaagcccgcc cgatcctggc tgcttcaccg caggcggatg ccggaccctc
     5161 actccgtgat cagatcatgg cactggatga ggcaaaaggg gctgaggcgc aggctgaaca
     5221 gctggctgcc tgcccgggaa tgactgtgga gagcgcccgg gctgtgctgg ctgcgggatc
     5281 aggtaaggca gaaccggtct ctgcatccac aaccgccctg tttgaacgca tcatggcgaa
     5341 ccattcaccg gctgcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga
     5401 cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga ccatcaacag gaggttttta
     5461 caatatggta acgaaaaaca tcactgaata gcgtgcggaa gtacgtattt ttgccggtaa
     5521 tgatccggct cataccgcca caggcagcag cgggatttcc tcggcaacac cggcactgac
     5581 gcccctgatg ctggatgaag ccagcgggaa actggtggtc tgggacggac agaaagccgg
     5641 tagtgcagtt ggcatactgg tactgccgct tgaaggcaca gagacggtac tgacctatta
     5701 caagtcgggg acctttgcga cggaggcaat ccgctggcct gaaagtgtgg atgaacacaa
     5761 aaaggcaaat gcctttgccg gcagtgccct gagtcacgcg gcgctgccgt aacacgttat
     5821 caggccaccg cggtggcctg actgatttct gaatgaaagg aactgattta tgggattgtt
     5881 tacgacccgc cagttactcg gttataccga acaaaaagtg aaatttcgtg cgctgtttct
     5941 ggagctgttt ttccgccgta cggtgaattt ccataccgaa gaggtgatgc tggacaaaat
     6001 taccggaaaa acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aagtgctgcg
     6061 tcatcgcggt ggtgaaaccc gcgtgttacg tccgggctac gtcaagccga aacacgaatt
     6121 cccctggagc cggtaaaagg agccggtacc accctgtggg tttataacgg tcagggtgac
     6181 gcctatgcaa acccgttgtc agacgatgac tggcagcgac tggctaaggt gaaggatctg
     6241 acgccgggcg agatgacggc agaatcctac gatgataact acctggatga tgaagacgcg
     6301 gactggaccg cgaccgggca ggggcagaaa tctgcaggtg ataccagttt tacgctggcc
     6361 tggaaaccgg gagaggaagg ccagaaaggg cttataggct ggtttgaaag cggcgatgtc
     6421 cgggcctata aaatccgttt tccgaatggc acggtggatg tgtttcgtgg ctgggtcagc
     6481 agtatcggta aggccgtgac ggcgaaagaa gtgatcaccc gcacggtgaa agtcactaac
     6541 gtgggtaaac cttctgtagc ggaagaacgc agcaaaatta cgccggtcag tgcgattaag
     6601 gtgacgccga catccggtac ggtggcaaaa gggaaaacaa ccaccctgac ggtttctttt
     6661 gagccggaaa gtgcaacaga caagacgttc agagcggttt ccgccgatcc gtcgaaagcc
     6721 accattagtg tgaaagatat gacaattacg gtaaacggcg tggcgacagg taaggtgcag
     6781 atccctgtgg tgagcggaaa tggtcagttc gccgcagtgg ctgaagtcac cgttactgaa
     6841 gcgggcgctg cagggtaaac ggaggtcata catgtttctg aaaacagaac aatttgaata
     6901 taacggtgtg tctgtcacgc tttccgaatt gtctgcgctg cagcgtatcg agcatcttgc
     6961 cctcctgaaa cggcgtgcag aacaggcaga atccagcggc aacctgcagg taagcgtgga
     7021 agatctcgtc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccaca
     7081 gaaaacgcag tcaccgtcaa tgaatgaggc cgtgatgaag atagagcagg aagtgctcac
     7141 cacctggcct gccgatgcca ttgcccgggc ggaagacgtg gtgttgtgcc tgtccgggat
     7201 gagcggggct gttcgtccgg atactgatat tactgaagtg gcgaaaaata acacgctgac
     7261 tgatgatgat ttttctgcgg gaaagtcttc gacggcgagc tgaactttgc cctcagactg
     7321 gcgcgtgaga tggggagacc cgactggcgc gccatgcttg ccgggatgac atccaccgaa
     7381 tatgccgact ggcaccgttt ttaccgcacg cattattttc aggataccca gctggatatg
     7441 catttttccg ggctgacgta cgctgtactc agcctgtttt tttgcgatcc ggatatgcat
     7501 ccctctgatt tcagtctgct tgtcccccgg catgaggaag agcaggtgga gaggccggat
     7561 gaggacaaaa tgctgatgca gaaagcggca ggacttgccg gaggcgtccg gttcggtggg
     7621 gacggagggc gcgatatttt atcgtctgcg gatgtggcgg atgtcatggt ggatgatgcc
     7681 gcattaatga tggcttcagc ggggattccg ggaggtgtga gatatgtccc agccggttgg
     7741 tgatcttgtt attgacctga gtctggatgc tgtccgtttc gatgagcaga tgagccgggt
     7801 aaggcgtcat ttttcaggtc tggataccga cgtcagaaaa accgccagtg ctgttgaaca
     7861 gggcctgagc cgccaggcgc tggctgcaca aaaagccggg atttccgtcg ggcagtataa
     7921 agcggccatg cgaaccctgc ccgcacagtt tacggatatc gccacgcagc ttgccggtgg
     7981 tcagaatccc tggctgatcc tgctgcaaca gggcggtcag gtgaaggact ccttcggcgg
     8041 gatgatcccc atgttcaggg ggcttgccgg tgcgatcacc ctgccgatgg tcggggtcac
     8101 ctcgctggcg gtggcgacag gtgcgctggt gtacgcctgg taccagggag attccacgct
     8161 ttcagcgttt aataaaaccc tggttctttc cggtaatcag tccggactga ctgccgatcg
     8221 catgctgacg ctctcaagag ccgggcaggc agcagggctg acgtttaacc aggcgagaga
     8281 gtcactggca gccctggtga atgccggtgt gcgtggtggt gaacagtttg atgccatcaa
     8341 ccagagtgtc gcgcgttttg cttctgcatc cggtgtggag gtggacaagg ttgcagaggc
     8401 tttcggaaaa ctgaccaccg accctacgtc ggggctgatt gcgatggtgc gccagttccg
     8461 taacgtgacg gcagagcaga ttgcgtatgt tgcgcagctg cagcgttccg gtgatgaggc
     8521 cggggcctta caggcggcga acgatatcgc cacgaaaggc tttgatgagc agacccgtcg
     8581 cctgaaagaa aacatgggga cactggagac ctgggcggat aaaaccggga aggcattcaa
     8641 atcgatgtgg gatgccattc tggatatcgg tcgtcctgag tcctcagcgg atatgctcgc
     8701 cagtgcacag aaggcatttg atgaggcgga taaaaaatgg cagtggtacc agagccggag
     8761 ccagcgccgg ggaaaaaccg cctctttccg ggccaacctt cagggcgcat ggaatgaccg
     8821 ggaaaatgcc cgtctggggc tggcagcggc cacgctgcag tcggatatgg aaaaagccgg
     8881 tgaactggcc gccagggacc gggccgaacg ggacgcatca cagctgaagt ataccggaga
     8941 ggcgcagaag gcgtatgagc gtctgctgac gccgctggag aaatataccg cccgtcagga
     9001 agaactgaat aaggccctga aagacgggaa aatcctgcgg gcggattaca acacgctgat
     9061 ggcggcggcg aaaaaggatt atgaatcgac gctgaaaaag ccgaagtcgt caggagtcaa
     9121 agtgtcagcc ggtgagcgtc aggaagacca ggcgcatgct gccctgctgg cgcttgaaac
     9181 cgagctcagg acgctggaaa aacacagcgg tgcgaatgag aaaatcagcc agcagcgtcg
     9241 cgatttatgg aaagcggaaa atcagtatgc ggtcctgaaa gaggctgcca cgaaacggca
     9301 gttatctgag caggaaaaat ccctgctggc gcataaagac gagacgctgg agtacaaacg
     9361 ccagctggct gagctgggcg acaaggttga ataccagaaa cgcctgaatg agctggcaca
     9421 gcaggcggtg cggtttgaag agcagcagag cgcgaagcag gccgccatca gcgcaaaagc
     9481 ccgcggtctc actgaccgtc aggcgcagcg ggagtctgaa gcgcagcgtc ttcgggacgt
     9541 gtacggtgat aatccggctg cgctggcgaa ggccaca
//
LOCUS       DAZFTN010000155         9414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFTN010000155 DAZFTN010000000
VERSION     DAZFTN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(49..360)
                     /locus_tag="ACHKV2_004449"
     CDS             complement(49..360)
                     /locus_tag="ACHKV2_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942209.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(353..580)
                     /locus_tag="ACHKV2_004450"
     CDS             complement(353..580)
                     /locus_tag="ACHKV2_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942210.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            complement(577..858)
                     /locus_tag="ACHKV2_004451"
     CDS             complement(577..858)
                     /locus_tag="ACHKV2_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942211.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            complement(891..1607)
                     /locus_tag="ACHKV2_004452"
     CDS             complement(891..1607)
                     /locus_tag="ACHKV2_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6942212.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(1641..2102)
                     /locus_tag="ACHKV2_004453"
     CDS             complement(1641..2102)
                     /locus_tag="ACHKV2_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6942213.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(2095..3148)
                     /locus_tag="ACHKV2_004454"
                     /pseudo
     CDS             complement(2095..3148)
                     /locus_tag="ACHKV2_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
     gene            complement(3217..3642)
                     /locus_tag="ACHKV2_004455"
     CDS             complement(3217..3642)
                     /locus_tag="ACHKV2_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6942214.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(3626..3868)
                     /locus_tag="ACHKV2_004456"
     CDS             complement(3626..3868)
                     /locus_tag="ACHKV2_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6942215.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            4260..4598
                     /locus_tag="ACHKV2_004457"
     CDS             4260..4598
                     /locus_tag="ACHKV2_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6942216.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            4891..5043
                     /locus_tag="ACHKV2_004458"
     CDS             4891..5043
                     /locus_tag="ACHKV2_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6942217.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            5055..5693
                     /locus_tag="ACHKV2_004459"
     CDS             5055..5693
                     /locus_tag="ACHKV2_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942218.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(5694..5903)
                     /locus_tag="ACHKV2_004460"
     CDS             complement(5694..5903)
                     /locus_tag="ACHKV2_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942219.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            6468..6656
                     /gene="dicB"
                     /locus_tag="ACHKV2_004461"
     CDS             6468..6656
                     /gene="dicB"
                     /locus_tag="ACHKV2_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6942220.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            6653..6841
                     /locus_tag="ACHKV2_004462"
     CDS             6653..6841
                     /locus_tag="ACHKV2_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6942221.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            6934..8172
                     /locus_tag="ACHKV2_004463"
                     /pseudo
     CDS             6934..8172
                     /locus_tag="ACHKV2_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            8160..8711
                     /locus_tag="ACHKV2_004464"
                     /pseudo
     CDS             8160..8711
                     /locus_tag="ACHKV2_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8889..9131
                     /locus_tag="ACHKV2_004465"
     CDS             8889..9131
                     /locus_tag="ACHKV2_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6942222.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(9128..9262)
                     /locus_tag="ACHKV2_004466"
     CDS             complement(9128..9262)
                     /locus_tag="ACHKV2_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFO6942223.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
     gene            complement(9293..>9414)
                     /locus_tag="ACHKV2_004467"
     CDS             complement(9293..>9414)
                     /locus_tag="ACHKV2_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO6942224.1"
                     /translation="LVKTRAAHPLSRESIAVSMIVGRDNCAFDSDRGNFVLKN"
BASE COUNT         2458 a         2411 c         2075 g         2470 t
ORIGIN      
        1 tttctgattt aaatcttcag tatttagttg ttagttggtt tatcgccttt atgcttcagc
       61 cttatttcgc aaccagacac aaaccgggcc atcttccgta tcatgaatgg aaccaataaa
      121 ccagccatcg ccctctggtc gttccggttc ccatgcagaa atatcagcat cacacgcatc
      181 aaggtcagca catccttcat ctctgaagca gaggacgtat tgaagattat tttcctccat
      241 ccaggcgtta aactcttccg ttgaaatata ttcccgacca tcacagaatt tttcatattc
      301 aggatgtgtc cagtagccat atccgtcacg aactaccggc atttctttaa tttcactcac
      361 tgttaacctc ctgcaacgct acacgatacg ccttctttat ccacgcctta ctgccatata
      421 atttcgtctt cataataaac acacctgcac gactcgccga tatccccgga caggttaaca
      481 gcacagcatc caccacacgg ttatgcttcc gaaactccat tacagtactg ctgataacca
      541 cctgccccac cgggccgtaa tcctgataca ggattttcac gcagacaccc tcctgtcgaa
      601 ataaacgtag ttattcacta tgcgcagcgg catgcctaat tttctggcaa tttcccttct
      661 ttgcatgcct ctctgatgca gttgccgcgc cagctcaata tcacgctgag aatattttgc
      721 cgacgggtga aaatcaccac gtaacatcat gctgatgccc agctcccgtg ccttcgttct
      781 cactgccgct tcagttcgtc cgataagtgc gccaacgctt tttaccttca tcgttcccgc
      841 acactgccgg agtatcatga tttccgcccg gcaccacgtc ttccacccac tcaccgctgc
      901 tgttctctgg tggcggtaat atcccggaga atatcccggc acttgttcag ctcccgcagc
      961 gcggcgcaga ctcgctccca cttctgaacc tgaccttttg cccggcgcag ctcgcggtta
     1021 gccacatgca gcgatggtaa aatcagacca tccggatgct ttctggtgaa cgacggctgt
     1081 gactgcactg tgaccgccac actttccgtt tttatttctt cctgtgtttc cgcttccccg
     1141 actggtaacg caacacctgc tggctgagga aaggctttac catccgtttc cgttatggat
     1201 gcggctttcg gctctgccgg taaatcagcg cccggtatgc agtaccgaaa tttaccgccc
     1261 tgattcacgc gaatcagacg ccctttgctg attgccatgg ccagtgatga attcgcccgg
     1321 cgggaggtaa tcccgaacat cagtgccagc tcatccgccg tttgtgggcc atgatgttca
     1381 atcgcctcag tcagcatttg cgctgtcact ttcggtaccg gtgacactgg ttcactttca
     1441 ccagtctgag tcagccacca catcgacccc ttgttatccg cttcaccacg gcgcttcagt
     1501 ttccacagtt cgttgacagc atcttcgcgg ctgattccaa ggcgcgatgc cactacctgt
     1561 gaagaggctt ttttcagtgc tttcagtgcg tcaaatacgg tttccattaa tatttcctcc
     1621 gacaaaatcg tttctcagat tcaaataaaa ccagctgcct tccggcgttc gtattcctgt
     1681 ttcagccgtt caattggcgt tggcccttgc gggtgtttcg ccccttccag ttgtcgtcgc
     1741 actggcggaa cactcatccc gttaccaaca tgctttgccc atttcgtcag ttgccgttcc
     1801 gcaagtcgtt ttaactcacc ttgcgtcatc tggcgctcaa tccctctggt acgcatttcg
     1861 aggcagatgt ggtacagcac aggctgtggc cacgggtatt tatcactccc gtcgtatcgc
     1921 caggattcat tgcgccagcg ccggtactct tccatcacgg catccaccgt aagaccaaat
     1981 ggatttgccc cactctccga aatcagcgca acaaactcag ccaggtccgg gggccacgtt
     2041 tcacccgccc ggcagcggtc catgcactga cggcacacca gacggatttg ctgttcagtc
     2101 atcgcaccaa tctgggcaat ccagagcttc gaaggtgcgg ccccgttctt ctgagtccag
     2161 cggttcgaat acacctcccc catgagctcc cacagcttcc aggccgtttc cgttgctgat
     2221 aaatccgttg tcacgttccc actgttcgcg tgcagcccgg atttcctgaa ctgcccgtga
     2281 tgccgtgcca cctgatgctg catggcttcc ccccttgctg actggtttta cctgtgccct
     2341 gacgtgctgc acgtggcggg caaatttctg ctcccactga acctgcgtga aaaccttccc
     2401 ctccgccatc cagtaatccc ggaatgcggc aagctcagca ggtgtaaatt ccggctcagg
     2461 cagagccata ccccacactg ctgcccgttg tcgaaaatcc ggcgacggct gccagacagt
     2521 agtcatcgaa aatttcccga tcggttcgct caggccgtcc aggtattcag gttcggctgt
     2581 ctgcaacggc gcaccattcg actcactggt cggaatactc tcgcgcgcgt tatgtgtggg
     2641 gtttaattct gtatctgtat ctttatctgt atctttatct gtcgtgactt gtcgtgactt
     2701 gtcgtgacag atgcgtgaca cgtcgtgact catcgtgaca atcagcatta tgtttccgca
     2761 gcttttcccg ctcccgctgc gctctcttgc gctctgccgg ggattttgcc gtttgcgaaa
     2821 cgttaccatt gtcctctttc agcacctgac gtttttccca tccggaaata aggtcaccat
     2881 ccagaacccg cccctgcatt gcatgcaaaa ttgaatcaat tacgtcttcc gtcacatcaa
     2941 gcgcacttgc taaatcttcc gtcgtgacat caatgtgacc acgtagtgac acgccgtgac
     3001 atgtcgtgac atttcgtgac gcgctcacca gaagatggat atacactgcc atcactgttg
     3061 cgattggctg tcctgatacc ctggcaatcg ttcgccactt ggggtcattt ggcatgtcat
     3121 gccacaatct gagccaggca ttagccatac tcacctcttc tgataccgaa ctttaccctc
     3181 gaacatccgg aagaaatccg gcatgaatat tgttggtcaa tgcacgacaa cagcattacc
     3241 aggctgaccc ccactgttag tcagggtgcc ccaggcgatc gccgctgcga caaaatcatc
     3301 cacatctttc accagccgat ccctccgttc gacgatctca cggtaatatt cagagctgtg
     3361 actgcgcata cgggccacca gcagaggcgg cattgccttt tcgatcgccg gtaacagagc
     3421 ctgcattttt tcaactgcat caggggtgtc tttctctatc cagcggaaaa ttttctgggt
     3481 attgcgagcc agggcttccg gatggctgtc gtcgtacagt tcaggaaacg tcatacccaa
     3541 ctcaaaataa gcctgggtta ttccagctgc cggaactttt tcgccatcag gacgcgccca
     3601 ggcattcatc gccatgcgga tgtgttcatg cttgattttc atgaatcccc ccttggttag
     3661 aaggcggatt atgatcagaa tcgggaatga caaccgtcgg tatgtgtaac tcatatttga
     3721 gcgccccggc agtgactgcc tgaattagca acgcccattt ccacggaacg tcttcccccc
     3781 acatgctgac tgtggttttt gacgttccta gagcggcggc tgttttaaca actccgccaa
     3841 aatagcctaa tacttctgat tttttcatga gtcgctccat aaaactgaac gccaaaagtt
     3901 taataatcaa aaccaaagaa agtcaagaaa caaaaccatc tgtgttttaa aatcaaaaca
     3961 tgaacaagca aacaatatct gaacgcataa cccaacgtat gcatgcgcta aacttgaaag
     4021 gcaaagacct tgtcaatgcc actggcgcat caaaaggctc cgtaagtcaa tggatgaacg
     4081 gtggaggagc gccgtcctcg cgttacataa gttcactggc aaagatattg aaagtaaacg
     4141 aaaattggct tcttaatgga ggagagttaa atacaggtga ttcgcttgat ctatctttac
     4201 cgccgataaa aacggttccg ctactatcac ttcagcaggc agcaagctgg agtgattata
     4261 tgaaaaattc ctcaataacc tcttgtgtgc agcttgtcgg agaaatcccg gccaatacct
     4321 ttgctgttgt tctagagagt gacagtatgt cgacatctgg tggtggagtt tccatcccaa
     4381 atggttcaac agtttttgtt gatcccgatc gaatcgtaca accaggaaat attgtccttg
     4441 ccttacccaa agggaccaca acgcctgtca ttcgcaaact ggagatagaa gggccggata
     4501 ttcttttagt ccccacgaat cctcgctacc cttcaattat gctggatgat ctatcttgca
     4561 tattgggagt atgctttaaa attcaacaag atatttaacc aacctcatct atttgattaa
     4621 ctgtatgcca tcgtagtgat ggcgtaacag ctgcctgctt aaaatgtttt gataaaaaaa
     4681 cattgacctt aaatgttcgt ttttctaaac ttcattcatt ccctcacccc gccccacaga
     4741 atgcagggca atacttcgag ttaccaggca gtggtcaggg gttaagtagc cagcccgagg
     4801 cgtaagaaca tgacggcagg gttcaacttt aataactatg cagcaggttt ttgttccgct
     4861 accccggcgt taaggggaaa tgaggtcaac atggatacgc tcaatcttgg caacaacgaa
     4921 tctctggtat gtggcgtgtt ccccaaccag gacggtacgt tcaccgcgat gacgtatacc
     4981 aaaagcaaaa cgtttaaaac tgaagctggc gcgcgtcgct ggttagccag aaacactgac
     5041 tgatgaggtt gacgatggaa tttaaagatt tacctccttc aatccaggag attgcagcac
     5101 acacacttcg tcatcgtctg aacgaacttg aattggaatc ggtaacgaaa aaagacactg
     5161 ataatatggc tcgtaatgtg cgcgatgcgt ttaccggatt gtatttctgt gcgtctataa
     5221 ataaacacga ctcagagagt gtggcaaata aaattgcaga aacgacagcg caaaacatca
     5281 atacgaaacc aacggaagaa gaaattgatc agtttgctca tgatgctggt ttaaaaaaca
     5341 agaaagaaaa atcgccatat gcggggaaca tgtttgttta tgacaatctc atcagaattc
     5401 gtggcgaaat tccggcggaa tacctggcaa gagtccatca ggcattgctt aaaaatttgg
     5461 aaacagaatt atttgatggc aacactaacg gtttcttcat ggtatcaggc cttgagaaag
     5521 actgggatgc agaaaaacgc tggaatgttg ctacatggtt attcagtaac agagccgctg
     5581 cactggaagc ttcggcatgt atttgcggcc tgttcttaac agaccacaaa tataatctgg
     5641 atgtgtacag ttatatttac gctgaacacg gtccactctg gattgactgg taattataag
     5701 gaaacaccag cagggccgcg gcgaccaaca gaacgattaa aatcaataat gccattataa
     5761 aggacattat ttaatttatc gtcgaatgct gattctgtga gcctcaactc tgaatgagtt
     5821 tttaataacc ctgattgcct gagttgattt accaggcatt caatctgttt ttcaataagc
     5881 ggatttcttt ttttgtttgg cattttatcc tccattgagg ttctgggtta aaaatggaga
     5941 ccaacacgct gtcacgtgtg gtcgtgcgcc ggacacggat aagaatccgg tactgacagt
     6001 ttactgaaag gatatttccc tgaaaagtca gtgcataacg cgaaagcgta cggcgaagct
     6061 ctttccctta gaaggcttgt cgttagattt cttcgaacgt gcgcttccgg ttgtggcact
     6121 ccgcgaaatg gcgcggcggt aagtatggcg gggttattcc ttccccattg aggacaccgg
     6181 gttgtcaggt tgaccatacg cttaagtgac aaccccgctg caacgccctc tgttatcaat
     6241 tttctggtga cgtttggcgg tatcagtttt actccgtggc tgctctgccg ccctttttaa
     6301 agtgaatttt gtgatgcggt gaatgcggct aagcgcacgc ggaacagtta aaaccaaaaa
     6361 cagtgttatg ggtggattct ctgtatccgg cgttaattgt taactggtta acgtcacctg
     6421 gaggcaccag gcactgcatc acaaaattca ttgttgagga cgcgataatg gaaacgttat
     6481 taccaaacgt taatacgtct gaaggttgtt ttgatattgg tgttctgctc agtaaccggg
     6541 agtttactga agatgccatt aacatgagga aatatgagcc ttatctgctc aatgataatt
     6601 ccatactttc ccgaattgct cttcttgaac ttggtatttt cggagaacgt caatgacttc
     6661 agcatttgca ctgatgatga cggtttttct tataacgggt gaatcacaga atgtgattac
     6721 cggaatttat gccagtaaag aatcctgcct ccaggcaaga gacgagcaaa aaatttctgg
     6781 tgaatgcctc ccggtaaaaa aagtatcgct gtacctgaat aacgaaacac cggctggata
     6841 accctccagc catattaaca ccataccaac ggattaaaaa tgccagcaat ggcagggatt
     6901 tgttcaccct taaatctgta atgaggttta tcaatgagca ctgataaaga agaatttgcg
     6961 ctatattgcg aagcaaaaaa tgacaaagtc agaaaacgtc tgggaattaa aggtggtttt
     7021 tactggacta cagcaaaaaa attatctgtt gccatctccc gctgcattac cgcaatggat
     7081 gacaacgatt atgatgaaga cgactttaaa aaacccgttc gcgtccattt acccgttgtg
     7141 aatgaccttc cacctgaagg cgtgtttgat accgaattct gcaaccgata cgaaaaaggc
     7201 ggggaagatg gcattacaat ggtatttatc gcgccctctc cctccgtgca ggagaaacca
     7261 gccagtactg acaataccaa cgtcaacggc gaagacatga cggagattga ggagaatatg
     7321 ctcctgccgg tttctggtca ggagctgccc attcgctggc ttgcgcaaca cggcagcgaa
     7381 aaaccagtaa cgcacgttgc acgggaagaa cttcaggcat tacatatagc acgggcggaa
     7441 gaactgccgg ctgttactgc cctggccatt tctcacaaaa caaagctgct cgacccgctg
     7501 gagattcgcg accttcacaa actggtacgc gacactgaca aagttttccc taatcccggt
     7561 aattcagacc tgggactgat aactgctttt ttcgaggcat acctggacgc tgactacact
     7621 gatcggggtc tgctgacaaa agagtggatg aaaggaaatc gtgtttcgcg tatcacccgt
     7681 acggcttccg gtgcaaatgc cggtggcggg aacaaaaccg atcgcaatcc gaatttagta
     7741 cacaccttcg atacgctgga tgtggagatt gcagcagcca cacttccgat ggattttaat
     7801 atttatgaaa ttccgggcag cgtttatcgt cgcgcaaaag aaatcgtcct gaaaagagaa
     7861 agtccgttca aagaatggtc cgcagcactt cgcgcaaccc cgggtattct ggactattcc
     7921 cgcgccgcta tttttgcact tatccggagc gctcaccctg agttttatca ctacccggga
     7981 cgccttcagg ggtatatcaa cgcctactta acggagactg atcacgagaa ccccagcaag
     8041 gaaactctca ctgctgcacg acatacaccg gaaaaagata tcctggaaga agttaaccgc
     8101 gaactgtctg ctaagcagga aacagaagaa gaaaatgatg aagaaaaacc gcaaccatct
     8161 tgcgcaatgg cagaatgata atcagaagaa atctggcaaa agaccgtttt acagtaccaa
     8221 aaacacaggc gggaaccaat cgtgtgattc accttattaa gcccgcaatc gacgctctcc
     8281 ggagtcagat ggcactaacg agactgagca aagagcatat catcgatgtt cacctcagag
     8341 aattcggcag aacagagaaa caaaaatgta cctttgtttt tcaacctgaa gtgtcagcga
     8401 aagtaaaaaa ttatggcgac cattttaccg ttgactcaat aaggcagatg tgggacgcag
     8461 cggtaaagcg tgccggaatc cgccatcgca aatcgtatca gtcgagacac acttatgcct
     8521 gctggtcgtt aacagcagga gctaacccgg cattcattgc aaaccagatg ggccatgcag
     8581 atgcgcaaat ggtgtttcag gtgtacggga aatggatgtc agaaaataat aatgcgcagg
     8641 taacgctgtt aaatacacag ttaagcgagt ttgccccaac catgccccat aacgaagcga
     8701 tgaaaagtta attttttatt tatcaattag ttagattgaa tgactcttga aatccataat
     8761 tcatagatgt tttttactat tctgtgggtt tttgggtgtt ttctaagttt tttcagatgg
     8821 ttgtattttt tctaaaaatc cctaatctcg attttgctgt ttatttgagg cctttttatg
     8881 tcccatatat gccccacaga taccccgcag ccaaaatcaa caaaatgcca aaaggttctg
     8941 ttcctgccct gcaacaagaa atgctgcgac gtgtcagtaa acgttatgac gatgtagaag
     9001 tgatcatcaa atccaccagc aacgatggcc tttcagttac tcgcaccgcc gataaagatt
     9061 ctgcaaaaac ttttgttcag gagacgctga aagatacctg ggagtctgct gacgagtggt
     9121 ttgttcgcta attaacgagt aaaatcagta acggctggaa atcattcaat actcgcacta
     9181 tcgaaagttc accagccaac cgcagcacgt tcttgcatac gaggtgccgc gttttacatg
     9241 ctgtaccgag aattaatttc attttacgaa aagttaaccc gccattgcta tcttaatttt
     9301 ttaaaacgaa gttacctctg tcagagtcaa aagcacaatt atctcttcct acaatcattg
     9361 aaactgcgat tgattccctg ctcaaaggat gagccgccct tgttttaaca agat
//
LOCUS       DAZFTN010000156         9340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFTN010000156 DAZFTN010000000
VERSION     DAZFTN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(423..953)
                     /gene="yodB"
                     /locus_tag="ACHKV2_004468"
     CDS             complement(423..953)
                     /gene="yodB"
                     /locus_tag="ACHKV2_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6942225.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1296..1946)
                     /gene="zinT"
                     /locus_tag="ACHKV2_004469"
     CDS             complement(1296..1946)
                     /gene="zinT"
                     /locus_tag="ACHKV2_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO6942226.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2203..2838)
                     /gene="msrQ"
                     /locus_tag="ACHKV2_004470"
     CDS             complement(2203..2838)
                     /gene="msrQ"
                     /locus_tag="ACHKV2_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO6942227.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(2839..3843)
                     /gene="msrP"
                     /locus_tag="ACHKV2_004471"
     CDS             complement(2839..3843)
                     /gene="msrP"
                     /locus_tag="ACHKV2_004471"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO6942228.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(3951..4364)
                     /gene="hiuH"
                     /locus_tag="ACHKV2_004472"
     CDS             complement(3951..4364)
                     /gene="hiuH"
                     /locus_tag="ACHKV2_004472"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO6942229.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4497..5168
                     /gene="hprR"
                     /locus_tag="ACHKV2_004473"
     CDS             4497..5168
                     /gene="hprR"
                     /locus_tag="ACHKV2_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO6942230.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5168..6526
                     /gene="hprS"
                     /locus_tag="ACHKV2_004474"
     CDS             5168..6526
                     /gene="hprS"
                     /locus_tag="ACHKV2_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6942231.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6634..7485)
                     /gene="hchA"
                     /locus_tag="ACHKV2_004475"
     CDS             complement(6634..7485)
                     /gene="hchA"
                     /locus_tag="ACHKV2_004475"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO6942232.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8077..9263)
                     /gene="ompC"
                     /locus_tag="ACHKV2_004476"
                     /pseudo
     CDS             complement(8077..9263)
                     /gene="ompC"
                     /locus_tag="ACHKV2_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2585 a         1942 c         2160 g         2653 t
ORIGIN      
        1 tttcgagcga aaatgtgcag ttattcagtc gttttcttat cgctcaccat tattctttta
       61 gacattcatc ctatgaaact gccgcaaagt tggtggtggg aactgaagtt gcgtagagaa
      121 ggggtcaaga attgagtcaa aaaaggaacg cggccgtcag attaaagccg cgggacaaag
      181 tccataattt ccaagagttg ttgaatttcc cagctttttt gaaagccttt gtatcatact
      241 aacaagtaaa aaaggtttaa ataccaaaat taacctgtat ttttgataag tcttaccatt
      301 gtacgattta cacattttct gctcctcctt gttgatagtg gaaagagtgc ggtatgcact
      361 tgcgtaaaca tttatgatgc gttgatgtcc atggttcaca tttcctttgt cagaggatcg
      421 acttatttgc ggcgaggcat cattcgtaac agagtattat ctttctgaat gtagtgatga
      481 aagagtgctg cgccagcgtg agctacgatt aaaaagtagc ctatatttgc ccaggtttcg
      541 tgaatatttt ttatcagtgc tttaatttcg ctgtttgggg taacaaaggg agacacattg
      601 aaaccaagga aactccacga ttttccactg taagccatca aagcaatccc cagcagagga
      661 agcgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt catttgccag
      721 gcaggtggcg gtggcacaat agaaggatca ctataacggt gttttataat caggcgtgaa
      781 aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat cagcagataa
      841 gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt tattgcaacg
      901 aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt catttttgta
      961 gctcctgtaa ttttcacagt caagctatcc cacgaacctt aaaaattcct tatacagatg
     1021 tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca gccaatataa
     1081 gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg aatccgttat
     1141 tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg atgagcgtgt
     1201 ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt atgatgacat
     1261 actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat ttcctcgacc
     1321 acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat ttcatttaat
     1381 aaagattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt tcgtggtgca
     1441 attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc tttacattcg
     1501 aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta tccatcgtaa
     1561 tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc gtcctcaatg
     1621 ccaatcatct cgatatctgt tgcatagcct ttgtgataat aatctttaat ttcagcaaat
     1681 gttttagttt tatctgcatc cgctttcttc tgaaagacgg gggcaagttt gccactttgc
     1741 agtaaaggat aaacggattg ccagactcca tcccagtcac tgagcgttcg gttttgtaca
     1801 ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa gggtttgccg
     1861 tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc taaagcaaca
     1921 gccagtttgt gaagacgaat cgccaaaaca gtttcctcca gagtcattgt atatgatgtg
     1981 taatgttata ttgtaacata tgacaaaatg caacaactct taggagatga gagcatagtt
     2041 ttctcaggtg tacttatcta tcttattgaa ataaaatgaa ttatgttgat aatttgaggt
     2101 tgatgacaaa cgatacataa cctgatgtga tctgcgtagg ccggataggg cgctcgcgcc
     2161 acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag ataatttgtt
     2221 gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcgac ttcttatacc gtaaggctaa
     2281 aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct tcactgacca
     2341 cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca actgttgcca
     2401 gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag caagcaaaat
     2461 aatccagctg ataattccta acgttaaata aggccgggta attaactcct tgcctaataa
     2521 cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca gtgtcgccca
     2581 ggcaaagcac cataagccta acaggcggcg tgtgcgtatc aataacggct gttttgcgta
     2641 gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag tgcgaccggt
     2701 aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga tcgcccagac
     2761 cagccagaga aacggcaaca atccggcaag atgcaggcaa actttcagcc atgtcacctg
     2821 ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat acagcgatgc
     2881 cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgtacat cgaggatgcc
     2941 gcctgaacca ataaatcgtt cggtagcctg tgaccagcgt gggtgatcaa catgcggatt
     3001 aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg ttggcggacg
     3061 ctcgcgggtc agcttaatac tgacgatcga tttaatccct ttaaagccat atttccacgg
     3121 cacaatcagt cgcaccggcg cgccattttg tggcggtaac gccttgccat aaacacctac
     3181 ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga cataaggata
     3241 tttcagcccg ccgccgataa agcggtcctg ctggcctggc atctgttccg gtgcataaat
     3301 tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg
     3361 cagcggaaaa ccaatccacg gcaccaccat cgaccacgct tccacgcagc gcatacgata
     3421 aatacgctct tccagcggga agcgacgggt taaatcatcg tgatcgaggg tcaatggttt
     3481 tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac cagcattagc
     3541 ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg agactttatc
     3601 tgctggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact ccagcgcttt
     3661 tccggcgggg gcgggtgggc gatcgttccc tttaaaccag ctaagcagat cggcatgcgc
     3721 agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca cctgccgacg
     3781 cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt gattcttttt
     3841 cataagccat cacacttttt gtcgttagcc agcaagagcc gtttactgtt aatctgtcag
     3901 ggagtgtcct ccctaacagt agcagagatc tgcttttaca tatttatgca ttaactgcca
     3961 cgataggttg aatacccata ttggctaagt aataaaggca catgataatg ctcgttcact
     4021 ttattaatat gaaattcaac ggggatctca gggaagaaac tttcaagatt ttgtttcttg
     4081 aaatagtccc cggttttaaa tacgacacgg taatcgcccg tagttgcagt ttgctcgggc
     4141 cacagtgcct taattcgtcc atccttatct gttttggcgg tattaagttg taaccagccg
     4201 ttgtccgctt tcttttcaag agtgactgtc acgtcggcag cgggttttcc tgtttgctgg
     4261 ttcaaaatgt gcacgctaag aatgttttgt tgtgcagcat agaccaaaga aggtaatgaa
     4321 aatgcagccg ttactacgga gagtactaaa taacgcttta acatgtttat atccttgtca
     4381 tgtgaatgag ttttgaggat agcgaaaaag cgtccagggt aaccttacag cagcattaca
     4441 attttgtaat gaaccaggct gtttctatga catatgattt atggcatatt attttcatga
     4501 agattttact tattgaagat aatcaaagga cccaggagtg ggtaacgcag gggctttccg
     4561 aagcgggtta tgtcattgat gccgtttctg atggcagaga tgggctttat cttgcgctga
     4621 aggatgatta tgcattgatc attctggata ttatgcttcc gggtatggat ggctggcaga
     4681 tcttacaaac gttaagaacg gcaaagcaaa cccctgttat ttgccttact gcaagggatt
     4741 ctgtcgatga cagagtcaga gggctggaca gtggagcaaa tgattatctg gtaaaacctt
     4801 tttcattttc tgagttgctg gcaagggttc gggcacaatt aaggcaacat cacgctttga
     4861 attcaacatt agaaatcagc ggcttaagaa tggactctgt tagtcaaagt gtgagcaggg
     4921 acaatatcag tattacactg acgcgcaagg agtttcagtt actttggcta ctggcctcca
     4981 gagctggcga aattataccc agaacggtta ttgcgagtga aatttgggga atcaactttg
     5041 atagtgatac caatacggtg gatgtcgcca ttcgcaggct ccgcgcaaaa gttgatgatc
     5101 cttttcctga aaagctaatt gccacaatcc gtgggatggg atattcgttc gtagcggtaa
     5161 aaaaataatg aaaagattat ctataaccgt ccgtttaacc ttgcttttta tattgctgct
     5221 gtctgttgct ggcgccggaa ttgtctggac tctctataat ggcctcgcaa gtgagttgaa
     5281 atggcgcgat gatacaacac tcattaaccg gacagcgcag atcaagcagt tattaattga
     5341 tggggtaaat ccagatacgt tacctgtata ctttaaccgg atgatggatg ttagtcagga
     5401 tatcttgatc attcatggtg atggcatcaa taaaattgtt aaccggacaa atgtcagtga
     5461 tgacatgtta aataacatac ctgctagtga gacaatcagc gcagctggca tttacagaag
     5521 cattattaat gatacagaga tagatgcttt acgaattaat attgatgaag tttcgccatc
     5581 attaacggtt actgtggcta aattggcttc agccagacat aacatgcttg aacagtataa
     5641 aatcaatagc attataattt gcattgtcgc cattatactg tgctcagtat taagtccgct
     5701 gttaatccga acggggttac gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa
     5761 ttataacgat agccgggagc ctgttgaggt tagcgcatta ccgagagaac taaaacctct
     5821 tgggcaggcg ttgaataaaa tgcatcatgc tttagtcaaa gattttgagc gtctaagtca
     5881 gtttgctgac gatctcgctc atgaacttag aacgccaatt aatgcattac tgggtcagaa
     5941 tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa
     6001 cattgaagag ctggaaaata tttcgcggtt aacagagaac atactgtttc ttgccagggc
     6061 agataaaaac aatgttttgg tgaaactgga ctcgctttct ctcaataagg aagtcgaaaa
     6121 tttgttggac tatcttgaat atctttcaga cgagaaagag atttgcttta aggtcgagtg
     6181 caatcagcaa atctttgcgg ataaaatttt actgcaacga atgttatcga atcttattgt
     6241 taacgctatt cgatattcgc cagaaaaatc gcgtattcat ataaccagtt ttcttgatac
     6301 caacagctat cttaatattg atatcgccag ccctggagcg aaaattaatg agcctgaaaa
     6361 actcttccgt agattttggc ggggagataa ttcgcgtcat tccgtaggtc agggacttgg
     6421 tctttcttta gtcaaagcga ttgccgaatt gcatggggga agtgctacgt atcactatct
     6481 caataagcat aatgtgttcc ggattacgtt gccgcaaaga aattaacata gggcttcagt
     6541 acgccataaa ttaaccgtga aaaaacgcag ccacgtcgtg agtactaacg cggctgcgat
     6601 tgattatgcg cttacattca aacgtaacag ggattaaccc gcgtaagctg ccagcatttc
     6661 ctgcgccgcc agtttaccca acgcattcgc tgcaaaagga ctgtcgccgg tgagaagttt
     6721 acggtcctta tgtactcgcc cggtgatgtc gtcattaatg atgttcatgc ccattttctt
     6781 cagttcttcg ccgaagtacc aggtgagatg acccggcata tagccaatct ctggcgtttg
     6841 tttgtctgcg gcgtctggga atgcgcaaat ggaataacca ttcagtggat tatcgccgtg
     6901 gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag ataacaaaac ggtcattttt
     6961 gattgcccac tgtaaagcgg cagctacgtc ctggctttca ggtaaaccaa taagtgcgcc
     7021 atgaccacca ggaacaaaga ttgctgcata ttcgctatca gcgttgaggc tggcgacaac
     7081 atccgcgagt ttcttcggat tgcggaacaa cgatttatgc tgctcaaaga atggcattac
     7141 tttttcatct ttctgcggca tagcccagta ttcaaacttg gtcatcagac cggaaatggt
     7201 cgccacttcg aattcgaaac ctgcagcatg gagatgatac aacggcagca acgtttcaat
     7261 cggatggtta ccggtcgaga acagttttcc gttatcggtc ggcaaataac gttcgtccgc
     7321 ggcgatcacc agaattttat gtttaccgcg ataaggtttt ggatagtcca cgccatcaag
     7381 atcagagaca ggactggtat attggctaag cgaatattct gaagggaaga atgcattatc
     7441 ttcagcaata tcgacctgcg gatttttact ggtttgaaca gtcatagtgt attccttatt
     7501 gttgcttagt tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg
     7561 ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg ggtgtaagta tgtaatgatg
     7621 atgctgcact ggtgttgaat attattcacg ccaaattttc cttattgagc aacttcgtcg
     7681 caagtggcct caatgatttt agaccattat cagaggtacg gggcggcggg gagagattta
     7741 gtgctgtgga atattgtggt gctgatgaat atttatatcg cggattggta gagaaaaagc
     7801 ttatttcatg cactattttt gcaaatattt ctatatggga agcgtgtttg ttacggatta
     7861 attcataaag aaatgcattt gggtgttggc ttgaagagtc gctggttgat gttattgtgc
     7921 caggaggggc agggtaaggg ggtgtatcga taagctttgg caattgcgtg tgccgcagat
     7981 ggagatgaaa tgccaataaa aaagcctgag ttcgatgagg gacgaactca ggctagtgtt
     8041 cagttttaca ataacacgcc gcggcgcgtt ggctgattag aactggtaga ctaagcccac
     8101 agccacgata tcatcggtag cgatgccgtt ttctttgtag aagtcgtcgt cgttgtccag
     8161 caggttgatt ttgtaatcaa cataagtgga catgtttttg ttgaagtagt aggtcatacc
     8221 aatatcaaca tatttaacca gatctttgtt ggtgtaatga tgattaccct ggctgtcgaa
     8281 gttatcacta tttacaccgc ctaaatcctt acctttagat tgcagataag cgatggacgg
     8341 gcgcagaccg aagtcgaact gatactgagc aacaacttca aagttttggg ttttgttagc
     8401 gatggcatca gcatcaccgt aaccagtcat gttacggggt tcagcataca tagctgctaa
     8461 gtaaacgtta ttagcgtcgt atttagcacc aacggtccat gcttctgctg tttcaccgcc
     8521 agcagttgca tcgaagcgat ctccataacg agtgttatga gtacctttgt gaacctgatt
     8581 gtcggtacgg tcagagttag agtaagcagc gcccagactt aaaccaaagt caaagtcata
     8641 ggaagtggac atactgaacc gtcgccgttc tctttagcaa gtttacgtcc attaccgtta
     8701 cctgaacctt cttgatcaaa taatccgctt ttctcgttgt taccctgata ctggagtgca
     8761 aagttcagac catcaacttg accaaagaaa ccattgttac gataggttgc tacgctgtta
     8821 gcacgaccgt tcatgaagtt gtcagcgcca gcataggtat ctccaccaaa ttctggcagc
     8881 atatcggtcc atgcttctac gtcatatacg acaccgtagt tacgaccata gtcgaaagta
     8941 ccattctgac caaagccgaa acctgcaaat gccagacgcg ttgcggtatt acccttgtcg
     9001 ctttcaggac cgtttgcgcc aatgttaaat tcccactgac cataaccagt gaattggtca
     9061 gcgatctgag tttcaccttt gaaaccgata cgggcatatg acatgtcgcc atcgctgcta
     9121 gcatcatcag agaagtagtg caggcccgct acttttccgt acaaatccag tttattgcca
     9181 tctttattat aaatttcagc cgcatttgct gcgccagcaa ctaataacgc cgggaccagc
     9241 attgccagaa cttttctttt cattatgtat tcccttgtga ttataatctt catgaatata
     9301 tcaataagtg ccgttatcca aaaaaaagca catttggata
//
LOCUS       DAZFTN010000157         9199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFTN010000157 DAZFTN010000000
VERSION     DAZFTN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            164..526
                     /locus_tag="ACHKV2_004477"
     CDS             164..526
                     /locus_tag="ACHKV2_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942233.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(705..1163)
                     /gene="ybbJ"
                     /locus_tag="ACHKV2_004478"
     CDS             complement(705..1163)
                     /gene="ybbJ"
                     /locus_tag="ACHKV2_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6942234.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1160..2077)
                     /gene="qmcA"
                     /locus_tag="ACHKV2_004479"
     CDS             complement(1160..2077)
                     /gene="qmcA"
                     /locus_tag="ACHKV2_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6942235.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2223..2900
                     /gene="fetA"
                     /locus_tag="ACHKV2_004480"
     CDS             2223..2900
                     /gene="fetA"
                     /locus_tag="ACHKV2_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO6942236.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2887..3666
                     /gene="fetB"
                     /locus_tag="ACHKV2_004481"
     CDS             2887..3666
                     /gene="fetB"
                     /locus_tag="ACHKV2_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO6942237.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3729..4583)
                     /gene="cnoX"
                     /locus_tag="ACHKV2_004482"
     CDS             complement(3729..4583)
                     /gene="cnoX"
                     /locus_tag="ACHKV2_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO6942238.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4644..5453)
                     /gene="ybbO"
                     /locus_tag="ACHKV2_004483"
     CDS             complement(4644..5453)
                     /gene="ybbO"
                     /locus_tag="ACHKV2_004483"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6942239.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5443..6066)
                     /gene="tesA"
                     /locus_tag="ACHKV2_004484"
     CDS             complement(5443..6066)
                     /gene="tesA"
                     /locus_tag="ACHKV2_004484"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO6942240.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6037..6723
                     /gene="ybbA"
                     /locus_tag="ACHKV2_004485"
     CDS             6037..6723
                     /gene="ybbA"
                     /locus_tag="ACHKV2_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO6942241.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6720..9134
                     /gene="ybbP"
                     /locus_tag="ACHKV2_004486"
     CDS             6720..9134
                     /gene="ybbP"
                     /locus_tag="ACHKV2_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO6942242.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2096 a         2369 c         2534 g         2200 t
ORIGIN      
        1 atttcgtgct ctttcaatgt aaaaaagagt aaagacttgt tttgataagc gaaataaatt
       61 ctgtttctgg actaaacgtg aagcggttcc tgatgtgtgc taaaatgcgt ttctttgata
      121 aatctcaatt tttaagaggt ttacgccagg caggaaaatt taaatgaatg aatttgagaa
      181 gatttttaat gaaatgaatc tggaccgggc attgctaccg atcctttttc gttcgaaccg
      241 gtcgacagtg tggaagtatt taagcggcga tagtacggct cctgcctctg ccatgtcgct
      301 gattatgctg ttgcagttga tacagaaacg aaaccccgat ctgttagccg aatggttaac
      361 gctttctgat ttcaccattc cgccagaagt ttacctggac cagcctgatt actggaaagg
      421 gtgggtgtat acgcaacaca aggtcaataa aaacgtcctg gaatatctaa aaaaacacta
      481 tccggatgaa gaccaaaaaa gtatgagtaa aggccgggaa gagtaaattg ccaatgccag
      541 ttaacattta acatggcgga ggcggcctac ggttttggcg tgatatgacg cgcccgcgtc
      601 gcatcaggcg tcggtgcacg actgccggat gcggcgtaaa cgccttatcc ggcctacgat
      661 tcgggcgcag acttccgggc atgataagac gcgtcagcgt cgcatcaggc gatgaccgca
      721 cggatgatca gcgttatccc ttctatcgca atgacttcaa catgcgtacc tgcgccgaga
      781 tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc gcatatgacc gcgcccgttg
      841 accagcggag attccagcac aaaacgtcgg ccaatcagct gctgcccgcg ctggtttaaa
      901 tgactgtcgc tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca ccacagccag
      961 gcggcgagca gcgtcaggac ggcaaacatc accccttgcc actcccaacc cagcggcacc
     1021 agccagacca ccaggccagt aatcactgct gccacgccac tccacaacaa ataaccattt
     1081 ccgcccagca tctcggctgc cagcagcaaa ccgccgagac tgagccagaa aatatgtgga
     1141 tgaacgacta ttaactccat catggctgag tccgcttgtt ggcgctgtct ttcaccagct
     1201 cggcaatccc ggcaatcgac cccatcaggc tgctggcctc taatggcatc atcactactt
     1261 tgctgttact ggaggaaccg atctgctgta acgcttcggt gtatttctgc gctacgaagt
     1321 agttcaccgc ctgaatatca ccggaggcga tggcttcaga caccattttg gtggcgcggg
     1381 cttctgcttc agcggaacgt tcacgcgctt cagcctgtaa aaacgccgac tgacgttcgc
     1441 cttccgcttt caggatttgc gactgttttt caccttcggc tttgaggatt tccgcctgac
     1501 ggatcccttc cgcttcaaga atgtaagcgc gtttggtacg ttccgctttc atctgcgcgt
     1561 tcattgaaga gataagctct gccggtgggc gcacgtcgcg aatttcaata cgggtgactt
     1621 taatccccca cgggttggtg gcctcatcga caatacgcag caggcgtgag ttgatgctgt
     1681 cgcgctgaga gagcatttcg tcaagttcca ttgaacccaa cacggtacgg atgttagtca
     1741 tggtcaggtt gatgatcgcc agctccagat tgctgacttc ataagccgcg cgtggcgcgt
     1801 caatcacctg aataaaacag actgcgtcga tggtaacgtt ggcgttatct ttcgagataa
     1861 cttcctggga agggatatcg agcacttgct ccatcatatt gatcttgcga ccaatgcgat
     1921 ccataaacgg caccaccaga ctgagccccg gctgtaacgt tttggtatag cgaccaaaac
     1981 gttctaccgt ccattgatag ccctgcggca cgattttgac gcccgcgccg acaatgacca
     2041 gcgcgacaaa aatgagaatc gggataaaga taagcatcgg aaaaacctcc tgttgtaccg
     2101 tccataatca gcaaaattgc tgcttgatta aacaaattat acctgattac tgaaagagag
     2161 ttccccctta ttcctgcgaa ggataaactg tttttagtaa aaatcagaaa aagggaacag
     2221 cgatgcagga aaatagtcct ttgcttcagc tacaaaacgt aggatatctg gcgggtgatg
     2281 cgaagattct taataacatc aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg
     2341 gtccttctgg ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg atcagcccaa
     2401 ccagcggaac gttactgttt gaaggtgagg atgtcagcac actaaagcca gaaatctacc
     2461 gtcagcaagt ctcttactgc gcccagacgc cgacgctgtt tggcgatacg gtatacgata
     2521 atctgatctt tccctggcag atccgtaacc agcagcctga cccggccatt tttctcgatt
     2581 ttcttgaacg cttcgccttg ccggacagca ttttgacgaa gaatatcgcc gagctatctg
     2641 gtggtgaaaa acaacgcatc tcattgattc gtaacctgca atttatgccg aaggttttat
     2701 tgctggatga aatcaccagt gcgctggatg aaagtaataa acataacgtt aatgagatga
     2761 tccatcgtta tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac gataaagacg
     2821 aaattaatca tgcggataaa gtgattacac tgcaaccgca tgccggagaa atgcaggaag
     2881 cacgctatga actcgcataa tattactaac gaatcattag cactggcatt aatgctggta
     2941 gtggtggcaa tcttaattag ccataaagaa aaactggcgc tggagaaaga tattctctgg
     3001 agcgtcgggc gcgcgataat tcagctgatt attgttggct atgtgctgaa gtatattttc
     3061 agcgtggatg atgccagcct gacattattg atggtgttat ttatctgctt taatgcggcg
     3121 tggaacgcgc aaaaacgcag taaatatatt gctaaagctt ttatctcatc gtttattgcc
     3181 attacggtcg gggcgggaat taccctggcg gtgctgattc tctcagggtc gattgaattt
     3241 atcccgatgc aggtgatccc tatcgccggg atgattgccg gtaacgccat ggtagcggtg
     3301 gggttgtgtt acaacaattt agggcaacgg gtcattagcg aacagcaaca gattcaggag
     3361 aaactgagtc ttggcgcgac gccgaagcag gcttcagcga tattgattcg cgacagtatt
     3421 cgcgcggctt taattccgac ggtcgattca gcaaaaacgg ttggcttagt gagtttacca
     3481 ggaatgatgt ccgggctgat atttgccggg attgatccgg tgaaggcgat taaatatcag
     3541 attatggtga cctttatgct gctctcaacc gccagcttgt cgaccataat tgcctgctat
     3601 ttaacctatc gtaagtttta taattcgcgc caccagttgg tggtgacgca attgaagaag
     3661 aaatgatgcg atgccggatg cgcagcatcg catccggcgt tgtggttcat gtgccgatca
     3721 acggaatatc aatacaacaa tgcatacagc tggcggcgat acttcgacgc cagtgcatca
     3781 cccgtaccca gcgcagcgag gatctcctgg aacgttttac gcgtctgacc gtctgcggcg
     3841 gtgagatctt tacgcagatg cccgaacagc aactccagcg cctcttcatt gcgcccaacc
     3901 tgatgcagtt gcagcgccag ttgcgtcgcc agtgcggcat cttctggatt ctccgccacc
     3961 tgctgttgca actgttgaat ttccggcgta tcagccgcct gcttcagcag ttcgatttgc
     4021 gccaccagcc cctggtagcg ggtgtcctga tcctgcaacg gaatggtttt cagcaccgct
     4081 tccgcatctt cagaacggtt cagcgcaatc agcgtttctg ccagcagcag gccgatctcc
     4141 ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg gcagggcatc ggtgtaattg
     4201 ccttcctgca tcagttgcat cgcctgctgc gctttcagct cttcttcgcg cggcagcact
     4261 ttatccagca gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc atctaccggt
     4321 tgcccgttct ggaacagata cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc
     4381 atctgctccg cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta ctgcgccgca
     4441 aggctttcca gaattggggt taactgcaaa cagtgctggc tacgttcaga ccaaaaatag
     4501 aacagcaccg gagtggtcat cgactgttca agaacctgtt gcaggttaga ttcgttaatg
     4561 ttgacaatat tttctacgga catggagtcg ctctctgttg tcgatatttt ctttgacatg
     4621 ggggcttaag cgcgcgcttc aactcacccc tgcaatattt tgtccatcac gcgccccggc
     4681 agcaggcgct taagcaccat taccgcccag gtcaccagcg ttaccggata gcgcatcttc
     4741 ggcttctcgc taataaaagc atggcgtact ttgtccacca ccgcttccgg tcccaacgta
     4801 aagcgggcgg cgatgccggg attttcgact ggtttatcac tttgcgtctg gttgacgttg
     4861 tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc tgactttaat tccgctgcgg
     4921 cgcagctcca tgcgcagcgc gtctgaccac gcctccagcg catatttact ggccgcgtaa
     4981 gcgccacgac ccggtgtgga gattaatccc atcaccgatg aggtcatcac aatacgccct
     5041 tcaccgtgcg gcaacatcgc gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag
     5101 ttggcggaaa actgctgttc catctgcgca cggctgatgg tggaaagggg gccatacatg
     5161 ccgaatccgg cattgttaaa gatcccatac agacaattat cggtcagggc gatcacctcg
     5221 tcggctgcgc gatcaacact ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc
     5281 atgctgttca tgcgctcaac atcatccggt ttccggcaac ctgccagcac atgaaaaccc
     5341 tggcgtttta attcgagcgc gctttccagg ccaattccac tggaacatcc ggtaattaag
     5401 accgattttt gcataacttt acctgtcagg atctccgttg ctttatgagt catgatttac
     5461 taaaggctgc aactgcttcg ccatccagtc ggcaataaac ggctgggcgt cgcggttggg
     5521 atgaataccg tcatcctgca tccattgtgg cttgaggtag acctcttcca ttaaaaaggg
     5581 cagcagcgga acatcaaact ctttggcgag tttggggtaa atggcgctaa aggcttcatt
     5641 ataacggcga ccatagtttg caggcagacg tatttgcatt aacaatggtt cagcgttggc
     5701 ggctttgaca tcctgcaaaa tctggcgcag cgtttgctcg gtttgctgtg gctgaaaacc
     5761 acgcaaaccg tcattgccgc ccagttcaac cagcacccaa cgcggctgat gctgtttcag
     5821 cagagccgga aggcgcgcca gcccttgttg cgaggtgtcg ccgctgatgc tggcattgac
     5881 taccgacgtt ttactctgcc acttatcatt caacaaggca ggccaggccg cgctggcaga
     5941 cattcgatac ccggcgctca ggctatcacc cagaatcaat aacgtgtccg ctgcggcagc
     6001 acggaaggtt aacaggacca gaaacaggaa gggcaaatgc cagcggaaaa cattgttgaa
     6061 gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg aactctccat cctcaccgga
     6121 gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg tgggcgagtc gggatcgggt
     6181 aagtcaacct tgctggcgat cctcgccggg ctggatgacg gcagcagtgg cgaagtgagt
     6241 ctggtgggac aaccgctaca taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag
     6301 cacgtcggct ttgtttttca gtcatttatg ttaattccta cccttaacgc gctggaaaac
     6361 gtcgagcttc cggcgctgct gcgcggtgag agtagcgcgg aaagtcgtaa cggagcgaaa
     6421 gcgttgctcg aacagttagg gctgggtaaa cgtctggatc atcttccggc acagctttcc
     6481 ggcggtgaac agcaacgagt ggcgctggca cgagccttta acggtcgacc tgatgtgctg
     6541 tttgccgacg aacccaccgg taaccttgac cgccagacgg gcgataaaat tgccgacctg
     6601 ctgttttccc tcaaccgtga gcatggcacc acgttgatta tggtgaccca cgacctgcaa
     6661 ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg ggcagttgca ggaggaagca
     6721 tgattgcacg ctggttctgg cgcgaatggc gctcgccgtc gctattaatt gtctggctgg
     6781 cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga
     6841 agggcttaag ccaacaaagc cgggagttta tggcgggcga tcgggcgttg cgcagttcgc
     6901 gcgaagtgcc gcaagcgtgg ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc
     6961 agctgacttt cgccaccatg acatttgcag gcgacacacc gcagctggcg aacgttaaag
     7021 cggtggatga tatctacccg atgtatggcg atctgcaaac caatccccct ggcctgaaac
     7081 cgcaggcggg cagcgtattg ctggccccac gcctgatggc actgcttaac ctgaaaacgg
     7141 gcgacaccat tgacgtgggc gatgccacct tgcggattgc cggagaagtg attcaggaac
     7201 cggattccgg ttttaacccc ttccagatgg ctccgcgtct gatgatgaat ctggcggatg
     7261 tcgataaaac cggagccgtg cagccgggga gtcgggtcac ctggcgctat aaattcggcg
     7321 gcagcgagaa ccagctcgac agctatgaga aatggttgtt acctcagctt aaacccgaac
     7381 aacgctggta cggtctggaa caggacgaag gcgcgctggg gcgatcgatg gaacgctcgc
     7441 aacagttcct gctgctttcg gcacttctga ccttgctgct ggcagtggca gcagtggcgg
     7501 tagcgatgaa tcattactgt cgcagtcgct acgatctggt ggcgatcctc aaaacgctgg
     7561 gggcagggcg agcgcaactg cgtaagctaa tcgtcggtca gtggttgatg gtgctgacgc
     7621 tttcagccgt taccggtggg gcgataggcc tgttgttcga aaacgtgttg atggtgctgc
     7681 tcaagcccgt tctgcctgct gcactaccgc cagccagcct ctggccatgg ctatgggcgc
     7741 tgggcaccat gacggtcatc tcgctgctgg tggggctacg accataccgc ttgttgctgg
     7801 caacgcagcc tttacgcgta ttacgtaatt atgtggttgc gaacgtctgg ccgctgaagt
     7861 tttatctgcc gattgtcagt gtggtggttg tgctgctgct cgccggatta atgggtggca
     7921 gcatgctgct ttgggcggtg ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc
     7981 tgggctggat gctgctgaat gtacttcgcc gcatgacgct gaaatcgctg cctctgcgcc
     8041 tggcggttag ccgcctgtta cgtcagccgt ggtcaacgtt aagccagctt tcggcatttt
     8101 cgctctcctt tatgctgctg gcactgctac tggtgctgcg tggcgatctg ctcgaccgct
     8161 ggcagcagca gttaccgcca gaaagcccga actacttttt aatcaacatc gccacagaac
     8221 aggttacgcc gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa tcgttttatc
     8281 cggtggtgcg agcgcggctg acggcgatta acggcaagcc gacagaaggc aatgaagatg
     8341 aggcgctcaa ccgcgaactc aatcttacct ggcaaaatac gcggcccgat cataatccga
     8401 ttgtcgccgg taactggccg ccaaaagctg atgaagtttc gatggaagag gggctggcga
     8461 aacgcttaaa cgttaccctc ggcgataccg tgacttttat gggcgatacc caggagttcc
     8521 gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt
     8581 ttattttccc tgaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct
     8641 gggagaatgg caacggcatg ttgacgcaac tcaaccgcca gttccctacc attagcctgt
     8701 tagacattgg cgcgatttta aaacaggtcg gtcaggtgct ggagcaggta agtcgggcgc
     8761 tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc
     8821 aggtgggaat gcgtcagcgt catcaggagc tggtggtgtg gcgcacactc ggtgcgggga
     8881 aaaaactgct gcgtaccacg ttgtggtgtg agttcgccat gcttgggttt gtttccggcc
     8941 tggtggccgc aattggtgcg gaaacggcac tggcagtgtt gcaggcgaaa gtgtttgatt
     9001 ttccgtggga gccagactgg cgattgtgga ttgttctgcc gtgcagcggt gcgctgctgc
     9061 tgtcgctttt cggcggctgg ctgggcgcga gactggttaa gggtaaggcg ctgttcaggc
     9121 agtttgcggg gtgatgagag taaataaggg gcgttctggt tgaatggaac gccttgttta
     9181 aattaattct acaatatgt
//
LOCUS       DAZFTN010000158         9150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFTN010000158 DAZFTN010000000
VERSION     DAZFTN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(345..1538)
                     /gene="tyrB"
                     /locus_tag="ACHKV2_004487"
     CDS             complement(345..1538)
                     /gene="tyrB"
                     /locus_tag="ACHKV2_004487"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO6942243.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1791..2870)
                     /gene="alr"
                     /locus_tag="ACHKV2_004488"
     CDS             complement(1791..2870)
                     /gene="alr"
                     /locus_tag="ACHKV2_004488"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO6942244.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2923..4338)
                     /gene="dnaB"
                     /locus_tag="ACHKV2_004489"
     CDS             complement(2923..4338)
                     /gene="dnaB"
                     /locus_tag="ACHKV2_004489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6942245.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4421..5404
                     /gene="qorA"
                     /locus_tag="ACHKV2_004490"
     CDS             4421..5404
                     /gene="qorA"
                     /locus_tag="ACHKV2_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO6942246.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5570..5812)
                     /gene="pspG"
                     /locus_tag="ACHKV2_004491"
     CDS             complement(5570..5812)
                     /gene="pspG"
                     /locus_tag="ACHKV2_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO6942247.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5946..6983)
                     /gene="dusA"
                     /locus_tag="ACHKV2_004492"
     CDS             complement(5946..6983)
                     /gene="dusA"
                     /locus_tag="ACHKV2_004492"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO6942248.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            7072..8169
                     /locus_tag="ACHKV2_004493"
     CDS             7072..8169
                     /locus_tag="ACHKV2_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6942249.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8231..8479
                     /locus_tag="ACHKV2_004494"
     CDS             8231..8479
                     /locus_tag="ACHKV2_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6942250.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(8490..8609)
                     /locus_tag="ACHKV2_004495"
                     /pseudo
     CDS             complement(8490..8609)
                     /locus_tag="ACHKV2_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8640..>9150)
                     /locus_tag="ACHKV2_004496"
     CDS             complement(8640..>9150)
                     /locus_tag="ACHKV2_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6942251.1"
                     /translation="VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGS
                     LTSREVTVLREIEMLENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTL
                     CEPEDGVFMRNSMNSNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDL
                     DRGNFVLKN"
BASE COUNT         2479 a         2305 c         2141 g         2225 t
ORIGIN      
        1 ctttaacttt agaatttaat gatactgaaa ttgaatgtca gaaagtcaca ggcaataagg
       61 caaagcatat tcttgaggat atagaaaaat tctatcgtaa caaacccgat acttattatt
      121 aaatttaata atggttagat gtgcagaaat tattactcca cacgaaaaaa acagggaaag
      181 gaattccaga taattgggat aaacttaaca ataaaagaac taacaacgtt tattatctct
      241 cacgtagaac gatggtatca aaaaaatcat ctataaagaa agagtgaaag cgacgacagt
      301 ttaatttcac tgcaggctgg gtaattccag cctgctttcc tgcattacat caccgcagca
      361 aacgcctttg ccacacgttg cacatttgcc gtatttaacc cggcgacaca catgcgaccg
      421 ctggcgatga gatagacacc aaattcttca cgtagtcggt caacctgagc ggcacttaaa
      481 ccggtataac tgaacatgcc gcgctgatta agcagataat cgaaattgcg ttctggcatc
      541 tctgtgctta ataccttcac cagttcctga cgcattgcca gaatgcgagt acgcatctct
      601 tctacttccg ccagccagct ggctttcaat gcctcgtcat tcagcactgc agccaccacc
      661 tgcgcaccaa aattcggcgg gctggagtag ttgcggcgaa ctgttgcttt caattgcccc
      721 agtacgcggc ctgcagcttc ggcatcttca cacagaacag aaagtccgcc gacgcgctcg
      781 ccgtaaaggg agaaaatttt cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg
      841 gcaatggcgc gaatggcgta ggcatcctct tccataccgg caccaaatcc ttgataggca
      901 atatcaagga atgggataag ctcgcgggct ttgagaattt caatcaccgc atcccactgg
      961 tcattagtga gatcggcacc cgttgggttg tggcaacatg gatgcagcaa cacaatactg
     1021 cgggcaggta atgttttcag catcgccaac aggtcattaa agcgcacgcc gttagtcgct
     1081 tcgtcatacc aggggtaagt gcttacttcg aatccagccc cggcgaatat tgctacgtgg
     1141 ttttcccagg taggatcgct gacccagacg cctgattccg ggaagtagcg tttcaggaaa
     1201 tccgcgccca ctttcaatgc ccctgagccg ccaagggttt gaatggttgc tacgcgctgt
     1261 tgttgcagta ccggatggtc ggcaccaaac agcagcggcg caatggcatg gcgatagctg
     1321 ttaagccctt ccatcggtaa ataaagcgaa gcgccatgag gcaccgcatt caggcgcgct
     1381 tccgcctccg ccacggcttt caattgtgga ataattccgt cttcgttgta gtacagaccg
     1441 atacttaaat tcactttgtc gctgcgaggg tcttctttaa aacgctccat aagcgtaaga
     1501 atcgggtcgc cagcgtaggc gtcaactttt tgaaacacgc gatggttctc caggtttacg
     1561 ggcaggtggt taaaacacaa taaaccggaa gaaggcgaag atcgagtgga tgttcagggg
     1621 cgaacggcaa ttagcaacag agtgagactc atgacaaacg tacatccgcc agaggcacga
     1681 cctttataag agcatgaaag aatatcaact tattgaattg gtaggatttt attggccgga
     1741 taaggcattc acgccgcatc cggcacagac aatcaaatat tacagaacga ttaatccacg
     1801 tatttcatcg cgacccttga agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc
     1861 atttcagcga tacgttctac gggcaaacct tcaccccaca aaatgaccgg gtccccggct
     1921 ttatcctgtg cctgtggacc taagtctacg cagatcatat ccatcgccac gcgcccgaca
     1981 atcggcactt cgcgaccgtt caccagcact ggcgtaccgg acggcgcggc gcgcggataa
     2041 ccatcgccat agcccatcgc aactacacca agacgagtat cacgttcgct tatccaggtt
     2101 ccaccataac cgacaggctc tccgacttta tgctcacgca cggcaatcag gctggaggtt
     2161 agtgacatca ctggctgaca gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag
     2221 acgccataaa gaatgatgcc cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga
     2281 atgccacccg acgcggcaat ggaacgttga cctggtttgc cttcgcaaaa ggtattaaag
     2341 atagcgagtt gtttctcggt tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg
     2401 acgatattca ccggctgccg aacgtttttg cactgggtca ggcgatgata aaacgcctca
     2461 gcctgttccg gccttacgcc cagacggtgc ataccggtat cgagtttcat ccagacggta
     2521 accggctcgt ccaggctagc ctcttccagc gcagccagct gttcttcgtt atgcacggcg
     2581 gtatgaaaat gttgcgcaga aatcgtcggc agatctctgg catcaaaaaa gccttcgagt
     2641 aacagtacag gtttggtgat tccccccgca cgcagtcgca gagcttcttc gagacgggct
     2701 acgccaaagg cgtcagcatc ggggagcgtt cgcgcggtct caagaagacc gtgaccataa
     2761 gcgttcgctt tcaccaccgc aaccatttta ctggcaggtg ccagttcacg aagacgttgc
     2821 aggttgtgtc gcagagcgcg gcggttaatc acaacagttg ccgcttgcat ttgtgttcct
     2881 tgataagtgt ttgctttaat tacctaattc ataaaataat tattattcgt cgtcgtactg
     2941 cggccccgca tagttgtcga agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc
     3001 gattgggccg ttacgttgtt taccgataat aatttccgcg atgcctttta aatcactgtt
     3061 ttcgtgatac acctcatcac gatagataaa catgatcaag tccgcatcct gctcgataga
     3121 gccagattca cgcaggtcgg agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg
     3181 gttcaactgg gacagcgcca ccaccggcac gttcagttct ttcgccagtg ctttcagcga
     3241 gcgggagatt tctgcaattt ccagcgtacg gttatcggaa agtgccggta cgcgcatcag
     3301 ttgcaggtag tcgatcatga taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc
     3361 gcgggaacgc acttccgttg gcgtcaggcc ggaggagtca tcgatataga tattgcgttt
     3421 ttcgagcaaa atacccatgg tgccggaaat gcgcgcccag tcttcatcat cgagctgccc
     3481 ggtacggatt ttagtctggt caacgcgcga cagcgacgcc agagaacgca tcatgatctg
     3541 ttctgatggc atctccagcg agaaaataag taccggttta tcctgcaaca tcgccgcgtt
     3601 ttcgacgagg ttcatcgcaa atgttgtttt acccatcgac ggacgcgcgg cgacgatgat
     3661 caaatccgac ggctgcaagc cagcggtttt tttgttgaga tcatcataac cggtgtttac
     3721 cccggtaacg ccatcgtgtg gctgctgaaa taactgctca atacgcgcca cggttgcgtc
     3781 gagcacatcg gcgatgttct tcggcccttc gtctttgttc gcacgacttt cggcaatttt
     3841 aaagacgcgg gattcagcca gatccagcag atcttcgctg gtacgcccct gcggatcaaa
     3901 accagcttcg gcaatctcat tcgcaaccga gatcatctca cggacaacgg cacgttcacg
     3961 cacgatgtcc gcataggaac tgatgttcgc agcacttggc gtattttttg acaactctgc
     4021 cagataagca aaaccaccga cgctatcgag ttgcccctgg cgttccagcg attccgcaag
     4081 ggtaatcaga tcgataggac taccgctttc ctgcaaacgc gccatttcag taaagatatg
     4141 acggtgtggg cgggtgtaaa aatcgtctgc taccacacgc tcggctacat catcccagcg
     4201 ttcgttatcc agcattaaac cgcccaacac cgactgctcc gcttcgatcg agtgcggagg
     4261 cactttcagc ccggcaactt gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa
     4321 gggtttattt cctgccatag tgaatggagt taccgagata aagaatgggt cgaaacttta
     4381 ccatatgaag cagaccctga cgatacgttc tggaggacac atggcaacac gaattgaatt
     4441 tcacaagcac ggtggcccgg aagtacttca agccgtagag ttcactcctg ccgatccggc
     4501 ggagaatgaa atccaggtcg aaaataaagc catcggcatc aattttatcg acacgtatat
     4561 ccgcagcggc ctttacccgc cgccatcact acccagcgga ttaggtaccg aagcggcagg
     4621 catcgtgagt aaagtcggca gtggtgtaaa gcatattaag gcaggcgatc gtgtagtcta
     4681 tgcgcagtcg gcgttaggcg cttacagctc tgtgcataac attaatgcgg ataaagcggc
     4741 gattctgcct gcggcaattt cttttgagca agctgcagca tctttcctga aaggcttaac
     4801 ggtttattat ctgctgcgca aaacctatga aattaaaccc gatgaacagt tcctgttcca
     4861 cgcagcagct ggcggcgttg gcttgattgc ctgccagtgg gcaaaagccc tgggcgcgaa
     4921 acttatcggc accgtaggaa ccgcgcaaaa agcgcagagc gcgctaaaag cgggcgcgtg
     4981 gcaggttatt aactatcgtg aagagaatct ggtcgagcgg ttaaaagaga tcaccggtgg
     5041 taaaaaagtg cgcgtagtgt acgattccgt gggcagagac acctgggaac ggtcgctgga
     5101 ttgcctgcaa cgccgcggct taatggtgag ttttggcaac tcatcaggtg cggttaccgg
     5161 tgtgaactta ggcattctca atcagaaagg ctcgttgtat gtgacacgcc cttccctgca
     5221 aggctatatc accacgcggg aggaattaac cgaggccagt aatgaactgt tctctttgat
     5281 tgccagcggt gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc
     5341 gcagcgtgcg catgagattc tggaaagccg cgcgacgcaa ggttccagcc tgttgattcc
     5401 ataaaagaaa tagggcttcc acctgggaag ccctttcttt ttatagttcg gctgtatgta
     5461 gggtacagca cgatgaatct gttagaggcg caatagtgac agatttgatt atcaattcct
     5521 attttgttct aaggataaaa ccttaggttg tgatcatccg cacaatccct tagtaacgcc
     5581 agcggtcata acgctgatat ttcggcactt ttggtgcttt aatcgcctta ataacccaca
     5641 ccaccgcaat cgccaggagt aaccacggca gcaacttaat catcaatgcc agcataccgc
     5701 cgaggaacat aatggccgtc gccacaacca gcgcggcgat aatgcccagc aacgaaacgc
     5761 cggtgaccat caacatgaca aaaaagccaa tcacaaaaag tagttccagc atgatgctct
     5821 cccaaatatg aaatctcttg caggcattac aagaatcatg ccaaaaataa tctattgatt
     5881 taacagcaaa acgccccgcg acggtgcgca gggcgtggtg aatttgacta ctttttggtg
     5941 aaaagttaac gcttatccgc caccagtttg agcgcgtgtt ccagcacgtt aatgtctgca
     6001 cccgctttat gggcattttc acttaaataa cgccgccact gccgcgcgcc aggaataccc
     6061 tggaacaagc ccaacatatg ccgggtaata tggccaagat acgtcccctg gctgagttca
     6121 cgctcaatgt acggatacat ggcgcgcact accgccaccg gatcggcatc ggtatccgag
     6181 gaaccaaaga tctctcggtc taccgccgcc agaatacccg gattctgata cgcttcgcgc
     6241 ccgaccatca cgccatccat atgttgcaaa tgcgctttag cctcttccag cgacttgata
     6301 ccaccgttaa tcgacatcgt cagatgcgga aagtcacgct tcagttgata cacacgcgga
     6361 taatcgagcg gcgggatttc acggttttct ttcgggctta accccgaaag ccaggcttta
     6421 cgtgcgtgga tgatgaacat ctcacactcg cctttgccgg aaacggtgtt gatgaaatcg
     6481 cagagaaatt catagctgtc ctggtcatcg atgccaatac gcgttttcac cgtcaccgga
     6541 atcgacacca catcgcgcat cgctttcacg cagtcggcaa ccagctgcgc attacccatc
     6601 agacacgcac caaacatgcc gttctgcacc cggtcagacg ggcagccgac attcaggttg
     6661 atctcatcat atccacgcgc ttctgccagc ttcgcacact gcgccagcgc cgccggatcg
     6721 ctaccgccga gttgcaacgc taccggatgt tcttcttcac tgtacgccag gtaatcacct
     6781 ttaccgtgaa taatcgcccc tgtagtcacc atttcggtat acagcaacgt attgcgggaa
     6841 agcagacgca agaaatagcg gcaatgtctg tccgtccagt cgagcattgg tgcaacgcta
     6901 aaccgagaat tccagtaaac accagttttt tcaggcatca cgctggtttg attaattttt
     6961 tgtgcttcat gattatcgtg catttttgaa catttcaggc tatttttctc gcgttaggtt
     7021 cccgcacagg ttcccacgtt ttatgggaac ccgaaataac gaggtcgtgt aatggcgtac
     7081 tataacatag agaaacgact aaaatccgat ggcacaccac gctatcgctg taatgtgatt
     7141 atcaaagaaa aaggtgttat cacttacagg gaaagcaaaa cattccctaa acatgctcat
     7201 gccaaaacat ggggcacaca gaaagtgatg gaattagatc tatatggcat tccatcatca
     7261 aatgcagttg acggacttac agtccgtgac ttactacaca aatatttaaa tgacccaaat
     7321 gccggaggta aagcaggccg tactaaaaga tatgtgctgg aactgcttat ggatagtgac
     7381 atatccgcga tcaaactatc tgaactgaca gaaaatgacg taattgaaca ttgcaggcta
     7441 agaaacaacg ctggtgcagg cccagcaaca gtcagccacg atgttagtta tcttggcagt
     7501 gttctggatg cggcaaaacc tgtatacgga atcaattaca catcaaaccc ggcgaaaagc
     7561 gctcgtccat atctacttaa actcggtttg attggtaaat caaaccgtcg taatcgtaga
     7621 ccagcatctg atgaacttga catgctcatt gaaggccttc aacaacgatc tactcataaa
     7681 tgctcaaaaa ttccgttcgt tgatatcctc aaattttctg tgtggtcctg tatgcgaatc
     7741 ggagaagtat gccggttacg atgggaagat ctcgaccaag aacaaaaatc tatactagta
     7801 agagatagga aagatccacg taaaaaggaa ggtaaccata tgaaagttgc cttgcttggg
     7861 gaagcctggg atatcgtcca gcgacaacca aaaaaatcag aattcatttt tccatataac
     7921 agcacttctg ttaccgcagg atttcagagg gtaagaagca aattaggtat taaagatctg
     7981 cgatatcatg atttgcgtag agaaggggca agtcgcttat ttgaggctgg ttttagtatt
     8041 gaggaagtcg ctcaagttac agggcatcgt tcattaaacg tgctatggca ggtatatacc
     8101 gaactgtatc cgaaatcttt acataatcgt tttgaagaac tccaaaagag cagaaacaag
     8161 acctcttgac actgtttatt tatacagata aaaataacac tgtatacaaa cacagtatag
     8221 agggattttt atgcgtattg aaatctgcat agccaaagag aaaatgacta aaatgccaaa
     8281 cggtgctgtg gatgcgttaa aggaagaatt aactcggcgc atcagtaagc gttatgacga
     8341 tgtagaagtg atcgtaaaag ccaccagcaa tgatgggctt tctgttacac gcactgcaga
     8401 taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg aatcttctga
     8461 cgagtggttt gttcactaat aaacacgtaa atcggtaacg gctggaaatc attcaatact
     8521 agcactatcg aaagttcacc agccaaccgc agtacgttct tgcatacgag gtgccgcgtt
     8581 ttacatgtcg taccgagaat taatttcatt ttacgaaaag ttaacccgcc attgctatct
     8641 taatttttta aaacgaagtt acctctgtca aggtcaaaag cacaattatc tcttcctaca
     8701 atcattgaaa ctgcgattga ctccctgctc aaaggatgag ccgcccttgt tttaacaaga
     8761 tgtattaatg ccattttatc atacaacata caaacatttg aattcattga attcctcata
     8821 aataccccat cttctggttc acataatgta acagggcatg ttcgtaagct ctctggcact
     8881 tgcaattgtg catgctccac attaaatgca catacttcaa ttttatctaa caactcctct
     8941 aactggtagt tagaaagcaa atccatattt tcgagcatct caatttcacg cagtactgta
     9001 acttcacggc ttgtcaaact accatgatta agtgtacgtt caatatgttc tatatattga
     9061 aggccaatct ggatattatc attcgataaa ccatcctggt taagacttcc cataaaatat
     9121 ccatttacct gcataggtcg tattgacacc
//
LOCUS       DAZFTN010000159         9106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFTN010000159 DAZFTN010000000
VERSION     DAZFTN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(142..837)
                     /gene="aqpZ"
                     /locus_tag="ACHKV2_004497"
     CDS             complement(142..837)
                     /gene="aqpZ"
                     /locus_tag="ACHKV2_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO6942252.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1263..2921
                     /gene="ybjD"
                     /locus_tag="ACHKV2_004498"
     CDS             1263..2921
                     /gene="ybjD"
                     /locus_tag="ACHKV2_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO6942253.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2918..3874)
                     /gene="ybjX"
                     /locus_tag="ACHKV2_004499"
     CDS             complement(2918..3874)
                     /gene="ybjX"
                     /locus_tag="ACHKV2_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO6942254.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4025..5140
                     /gene="macA"
                     /locus_tag="ACHKV2_004500"
     CDS             4025..5140
                     /gene="macA"
                     /locus_tag="ACHKV2_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO6942255.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5137..7083
                     /gene="macB"
                     /locus_tag="ACHKV2_004501"
     CDS             5137..7083
                     /gene="macB"
                     /locus_tag="ACHKV2_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO6942256.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7156..7380)
                     /gene="cspD"
                     /locus_tag="ACHKV2_004502"
     CDS             complement(7156..7380)
                     /gene="cspD"
                     /locus_tag="ACHKV2_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO6942257.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7530..7604
                     /gene="yljB"
                     /locus_tag="ACHKV2_004503"
     CDS             7530..7604
                     /gene="yljB"
                     /locus_tag="ACHKV2_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO6942258.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7785..9008
                     /locus_tag="ACHKV2_004504"
     CDS             7785..9008
                     /locus_tag="ACHKV2_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6942259.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRSGTGRQISPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLDV
                     LAGNHENIVMLKVAK"
BASE COUNT         2316 a         2174 c         2414 g         2202 t
ORIGIN      
        1 gtaagacgcg ccctcagccc acatccatgt gggctgcccc ggccttcggg gaacgcttca
       61 gcgattttga cgccaccaat acccgtgcgg tttgtaatgt cgaagataaa acaaccaggg
      121 cagataaaat gccaatgcta attaattacg cttttccagc agagtccggt aaatcagacc
      181 accgataatg ccaccgacaa ttggcaccac ccagaagaac cacagttgtt ctaatgccca
      241 gccgccctgg aagatggcaa ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac
      301 cggaatactg attaagtgaa tcagggttaa ggccagacca atagcgatcg gcgcaaaacc
      361 tgccggagcg aatttgtcgg ttgcaccgtg gatcactaac aggaagcccg cactcaatac
      421 cagttcaact accagcgcgg aaagcatgga ataaccgcct ggtgaatgct cgccataacc
      481 gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg caattaaata
      541 cagcagcgct gccgcaacaa taccgccgac aacctgggca attacgtagc caacgacttc
      601 ttttgccgga aaacgtccgc cagcccataa accaatagtg accgccgggt taaaatgacc
      661 accagaaata tgaccaacag caaaggccat cgtcagaacg gtcagaccga acgccaacgc
      721 cacgccggca aaaccaatgc ctaattccgg gaagcctgcg gccagtacag cactaccaca
      781 gccaccaaaa acaagccaga aagtaccaaa acattcagct gctaattttc tgaacatatc
      841 cacctcaatt aaaaattgac cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa
      901 aagtgacgac ggaaataatg cgcggctatt ttaaaaacga aggcgagtca ttcaccagat
      961 aaataaatcc agtaaatttg atttagggca acagcgggtt gccccatata gtcatttgtc
     1021 tgattgacag tgtagtgcaa gcaaaagatt taatccttta ggcgtaataa aaaataattt
     1081 atcatgctaa ttatttgatt ttgttgtttt tgcagactta tcagcaagag ggagtataac
     1141 gcgattattc gctcattttt cagacatttg ccatgcttaa atgtgatgtc atcacgtatt
     1201 agcaaggcct ttcccgttat actgccagcg taaaggataa gtcacatatt tctggagggg
     1261 atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga
     1321 tgctggaaca aaacaacgtc ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg
     1381 acgccttaac cctgctgcta tcgccagaat cagatctcta ccattttgag cgcgacgatt
     1441 tctggttccc gccgggagat atcaacgggc gagaacatca tctgcatatt attttgacct
     1501 tccgcgaatc gctgccaggt cgacatcggg ttcgccgtta tcggccgctg gaagcgtgct
     1561 ggacgccatg caccgatggc tatcaccgta ttttttatcg tctggaaggg gagagtgcgg
     1621 aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa agacggacat ccgattgatg
     1681 ttgaggatat taacgatcag gcacaccatc tggtgcgttt aatgccggtg ctgcgcttgc
     1741 gtgatgcccg ttttatgcgc cgtattcgta acggcacggt gccaaatgtc cccaatgtgg
     1801 aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt gtcctcacat ccgcaaaatc
     1861 tctctgatgg acagattcgt cagggacttt ccgcaatggt gcagttgctt gagcattatt
     1921 tctctgagca gggggctgga caggcgcgat atcgtttaat gcggcggcgg gccagcaatg
     1981 agcaacgaag ctggcgctat ctggacatca tcaaccggat gattgaccga cctggagggc
     2041 gctcgtatcg ggttattttg ctcggcctgt ttgctacttt attgcaggca aaaggcacat
     2101 tgcgactgga taaagacgcc cgtccattgt tgctgatcga agatccagaa acgcgtttac
     2161 accccattat gctttcagtt gcctggcatc tgttgaatct tttgccttta caacgtattg
     2221 ctaccaccaa ctctggcgag ctgctttcgt taactccagt ggaacatgtt tgccgcctgg
     2281 tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc aagtggcttg agcaccgaag
     2341 acagccgccg catctctttt cacattcgtt ttaatcgtcc gtcatcgctg tttgctcgct
     2401 gctggttgct ggtggaaggg gaaacggaaa cctgggttat caatgaactg gcgcgtcagt
     2461 gcggacatca ttttgatgcc gaagggatca aggtcattga gtttgcccag tccgggctaa
     2521 agccactggt taaatttgcc cgccgaatgg ggattgaatg gcatgtactg gtcgatggcg
     2581 atgaagcagg gaagaaatat gccgctacgg tacgcagcct gttgaataat gatcgggaag
     2641 ccgaacgaga acatttaacg gcgttaccgg cgctggatat ggaacatttt atgtatcgcc
     2701 agggattttc cgatgtgttc caccgcgtgg cgcaaatccc ggaaaatgtc ccgatgaatc
     2761 tgcgcaaaat tatctcgaaa gcgatccatc gctcttccaa acccgatctt gccattgaag
     2821 tggcaatgga ggccggacgt cgtggtgtgg actccgtacc gacgctgctg aaaaaaatgt
     2881 tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta accgcgaaac atcgtggcca
     2941 tttgtggctg aatagcgtcg agcatctcat agcgccgacg gtattcagcc cgttttttac
     3001 tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg aaggcggtaa tggcgttcag
     3061 catcacatac gccgccaacc gattcccaga aagcgttgta atcggcatgg atcttgcctt
     3121 ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc attgctgacg gcaataatct
     3181 gctctacctg caaacgttgg gcaaacagac aggccgcttc catcacgagg cgtttgggaa
     3241 atagcccgtg gcaggctttc gtcgcattct ggatttcctg atgtggaatt tcccattttg
     3301 cgccttgcag tccaccaata aacatcgttc ttttcccctg atattcacac agggtaaacg
     3361 tgatttctgc cagaggaata ccttcgctgt tgcggaacag gattgtgctg tcaccttctt
     3421 tatccattga gatcatcatg gtcagctcaa gcgtgaactg ctcgccgttt ttgccttcca
     3481 gcttcgccag ttgcagcccg ggggtattca aatataagct gaattcttcc gccgacatac
     3541 atccacggag taacgcataa tggtaacgta acgcctccag caattgctta cggctaagat
     3601 tcgccgcaag gtaagggcga tgcagacgca caggcagtcg cggctggcgc gttaacaata
     3661 cattgagatt aggccagtgg gaaagttcgt tcatccactc aacgcttaaa cgcggcataa
     3721 tcaacgagcg cagcaaaaat ttctggcgaa aactacggcg atgccagaat ttacccggcc
     3781 gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct gctgagagat tcagatgacg
     3841 taaaggtccg ttcggttagc tgcgacatat tcatgaaatc aatggttgta catgacgtcg
     3901 atttcaccat tgcgtatctt aaccaaacat caatagtgtg attactaacg taaattttag
     3961 ggttttgttg atatttcgtt gaagttaatg acccggattg gcatatggag tattcagaat
     4021 atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt attgcgctgg tgatagtcat
     4081 cgccggactg attacgttat ggagaattct taacgcaccc gtgccgactt atcagacact
     4141 gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg accggaaagc tggacgcgct
     4201 gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg aaaacgctgt cggtggcgat
     4261 tggcgataaa gtaaaaaaag accagctttt aggggttatt gatcctgaac aggctgaaaa
     4321 ccagatcaag gaggtcgaag caacgctgat ggagctacgc gcgcagcggc agcaggcgga
     4381 agcggagctg aaactggcgc gggtgacgta ttcccgtcag caacgtctgg cacaaacgca
     4441 ggcggtttca ctgcaggatc tcgacaccgc cgcgacggag atggctgtga aacaggcgca
     4501 aattggcacc attgacgcgc aaatcaagcg caatcaggct tctctcgata cggctaaaac
     4561 caatctcgat tacactcgca tcgttgcccc gatggccggg gaagtcacgc aaatcaccac
     4621 tctgcaaggc cagacggtga ttgccgcaca acaagcaccg aacattctga cgctggcaga
     4681 tatgagcacc atgctggtaa aagcgcaggt ttctgaagcg gatgtaatcc acctgaagcc
     4741 ggggcaaaaa gcctggttta cggtacttgg cgatccactg acgcgctacg aggggcaaat
     4801 caaggatgta ctaccgacgc cggaaaaggt taacgacgct attttctatt acgcccgttt
     4861 tgaagtcccc aaccccaatg gtttgctgcg gctggatatg actgcgcaag tgcatattca
     4921 gctcaccgat gtgaaaaatg tgctgacgat ccctctgtcg gcgttaggcg atccggttgg
     4981 cgataatcgt tataaagtca aattgttgcg taatggtgaa acacgcgagc gtgaagtgac
     5041 gattggcgca cgtaacgata ccgatgttga gattgtcaaa gggctggaag cgggcgatga
     5101 agtggtgatt ggtgaggcca aaccaggagc tgcacaatga cgcctttgct cgaattaaag
     5161 gatattcgtc gcagctatcc tgccggtgat gagcaggttg aggtgctgaa gggcatcacc
     5221 ctcgatattt atgccggtga gatggtagcg attgttggcg cttcgggttc cggtaaatcg
     5281 accctgatga atattctcgg ctgtctggat aaggccacca gcggcaccta tcgcgtcgcc
     5341 ggtcaggatg ttgccacgct ggacgccgat gcgctggcgc aactgcgccg cgagcatttc
     5401 ggctttattt tccagcgtta ccatttgctt tcgcatttaa ccgccgagca gaacgttgaa
     5461 gtaccagccg tctatgctgg tcttgagcgg aaacagcggc tgcttcgcgc ccaggagttg
     5521 ctgcaacgac tggggctgga agaccgtaca gagtattatc cggcacagct ttcgggtggt
     5581 cagcaacagc gcgttagcat cgcgcgggca ttgatgaacg gcggtcaggt aattcttgcc
     5641 gatgaaccga ccggcgcact ggacagccat tcaggcgaag aggtgatggc gatcctgcat
     5701 cagctgcgcg atcgcgggca tacggtgatt atcgtcactc acgatccgca ggtcgctgct
     5761 caggccgagc gggtgatcga aattcgcgac ggcgaaattg tgcgcaatcc tcccgccatt
     5821 gaaaaagtga atgttgctgg cgggacggaa cctgttgtca acacggcgtc tggctggcgg
     5881 cagtttgtca gcggttttaa tgaggcgctg acgatggcat ggcgggcgct ggcagcgaat
     5941 aaaatgcgta ctttactgac catgctgggg attataatcg gtattgcgtc ggtggtttcc
     6001 attgtcgtgg tgggcgacgc cgccaaacaa atggtgctgg cggatattcg ttctattggt
     6061 acgaatacta ttgatgtcta tcccgggaaa gattttggcg atgacgatcc gcaatatcaa
     6121 caggcgctga agtacgatga cttaatcgcc atccaaaaac aaccgtgggt cgcttcagcc
     6181 acacctgccg tctcgcaaaa cctgcgcctg cgttataaca atgttgatgt tgctgccagt
     6241 gccaatggcg tgagcggcga ttattttaat gtctatggca tgaccttcag tgaaggaaac
     6301 acctttaacc aggaacagct gaacggtcgt gcgcaggtcg tggttctcga cagtaatact
     6361 cgccgccagc ttttccccca taaagcagat gtggttggcg aggtgattct ggtcggcaat
     6421 atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt cgatgtttgg tagcagtaaa
     6481 gtgctgcgtg tctggctacc ttacagcacg atgtccgggc gagttatggg ccagtcgtgg
     6541 ctgaactcca ttactgtcag ggtgaaagaa ggatttgaca gcgccgaggc ggaacagcaa
     6601 ctcacgcgtt tactttcact gcgccacggt aagaaggatt tctttacctg gaacatggac
     6661 ggcgtcttga aaaccgttga aaagaccaca cgtactttac aactgtttat gacgctggtg
     6721 gcggtgattt cgctggtagt gggcggtatt ggtgtaatga atattatgct ggtgtcagtg
     6781 accgagcgga cgcgggaaat tggcattcgc atggctgtag gtgcgcgagc aagcgatgtt
     6841 ttgcaacagt tcctgatcga agccgtactg gtttgcctgg tcggtggcgc gttgggaata
     6901 acactgtcac tgttaattgc tttcaccttg cagcttttct tacccggctg ggagattggt
     6961 ttttcaccgt tggcactgct gctggcgttt ctctgctcga cggtcaccgg gattttattt
     7021 ggctggttac ccgcacgaaa tgcggcacga ctggatccag tagatgctct ggcacgagag
     7081 taatttttga gataaaaatg ccagccgatc gggctggcat tttgccttta ggatgtacac
     7141 aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa taacactggc
     7201 gtgattgcct tttggcccct ggtggacatc aaactgaacg gattgtccag cttttagcgt
     7261 tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc cgccgccttc
     7321 agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac ccttttccat
     7381 gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg gtcagagagg
     7441 gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg gcgagccgtc
     7501 aagcatttga caggggacag ggggcaggta tgaatcaaaa atttgaagca gttaacgcta
     7561 ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta tttatcagtt
     7621 agtgttcgtg ggtgttcgtc atggtgtggg catctaattt atttctatga ttataaagga
     7681 taatttcgtt tttcttgttc gcccttgttc gtcacagttc gcccttgtac gatgcacggt
     7741 gtagtcaatg gtgtagttaa ttttgatgac cggaaacacg aaacatgagc agggcaattc
     7801 atagacttag cgacactctt ttacgaaaat taagcggatc accaaccaca aaaagcacct
     7861 tttttaatga tggtggcaac cttagcgtaa ggcattcaac caatggcctg ttaacctggt
     7921 atttcactta caggtccgga actggtaggc agatatcacc tgaacgtctg aggctgggaa
     7981 attatccaga tttgagcctg aaagcagcca gggaaaaagc gtcgcaatgt cgcgcatggc
     8041 tggctgaggg gaaaaatccg cgctatgaac ttaatcatgc tgtacaggac gcgttagccc
     8101 ccgtgacggt taaggaagcg ctcacttact ggcttgaatc gtacgcaaag gaaaagcgca
     8161 ctgactacga atcactgaaa agccggatca ataaacacat aattagccag attggagcta
     8221 tgccactgga aaaatgcgag ctacgccact ggctggcctg ttttgaccag cttgctaagc
     8281 gtaatccggt atcagccgga tttttgctac aggtatgcaa acaggcgctt aagtactgcc
     8341 gaaaacgacg atacgcaatc agcaacgttc ttgatgatat ggtggtcggt gatgttggga
     8401 aaaaagcaga aataagcgag cgcgtactaa caaacaagga gcttggggaa ttactacgcg
     8461 cccttgatga aaaaatttac ccaccgtact acagcgccct gattcgcctc ctgattgtct
     8521 tcggatgtcg taccactgag ctaagacgct ctgaggtcca ggaatgggat tttaaagaaa
     8581 tgctctggac cgtgccgaaa gaacacagca aaacgaaggt agcaatattc aggccaatac
     8641 cggaagcgat cttgccgttt gtcatgaagc tggtggagca gaacaggcac acgggcttat
     8701 tgctcggaga actgaaaggg caatcatccg tatcagagta cggaagaacg gcacacagac
     8761 gcattaatca agccccctgg acgttgcacg acatccggca cacgtttaca acgatgctga
     8821 acgatttagg cgtggaaccg catgttgtgg agcagcttac cgcgcaccag ttgccaggaa
     8881 tgcaacgagt ctataatcac tcccgttatc ttgatgcaaa acgtgacgct cttaatctat
     8941 gggttgagcg cctcgatgtg ctggctggaa atcatgaaaa tattgttatg ctgaaggtgg
     9001 ccaaatgaaa ttacctaaaa gactctatta tccgttacct gaagcggcag aaaagttagg
     9061 ctgtgcaata aaagatcttt atcattacgc atatataggt gctctt
//
LOCUS       DAZFTN010000160         9091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFTN010000160 DAZFTN010000000
VERSION     DAZFTN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHKV2_004505"
     CDS             complement(<1..643)
                     /locus_tag="ACHKV2_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942260.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFF"
     gene            complement(659..1063)
                     /locus_tag="ACHKV2_004506"
     CDS             complement(659..1063)
                     /locus_tag="ACHKV2_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6942261.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(1153..1290)
                     /locus_tag="ACHKV2_004507"
     CDS             complement(1153..1290)
                     /locus_tag="ACHKV2_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942262.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            1362..1565
                     /locus_tag="ACHKV2_004508"
     CDS             1362..1565
                     /locus_tag="ACHKV2_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942263.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            1587..1937
                     /locus_tag="ACHKV2_004509"
     CDS             1587..1937
                     /locus_tag="ACHKV2_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6942264.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            1948..2226
                     /locus_tag="ACHKV2_004510"
     CDS             1948..2226
                     /locus_tag="ACHKV2_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942265.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            2238..2480
                     /locus_tag="ACHKV2_004511"
     CDS             2238..2480
                     /locus_tag="ACHKV2_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO6942266.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            2477..2590
                     /locus_tag="ACHKV2_004512"
     CDS             2477..2590
                     /locus_tag="ACHKV2_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942267.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            2683..3099
                     /locus_tag="ACHKV2_004513"
     CDS             2683..3099
                     /locus_tag="ACHKV2_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942268.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            3123..3326
                     /locus_tag="ACHKV2_004514"
     CDS             3123..3326
                     /locus_tag="ACHKV2_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942269.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            3323..3589
                     /locus_tag="ACHKV2_004515"
     CDS             3323..3589
                     /locus_tag="ACHKV2_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6942270.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            3586..3885
                     /locus_tag="ACHKV2_004516"
     CDS             3586..3885
                     /locus_tag="ACHKV2_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942271.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            4208..4438
                     /locus_tag="ACHKV2_004517"
     CDS             4208..4438
                     /locus_tag="ACHKV2_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6942272.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            4511..4876
                     /locus_tag="ACHKV2_004518"
     CDS             4511..4876
                     /locus_tag="ACHKV2_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942273.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            4883..7705
                     /locus_tag="ACHKV2_004519"
     CDS             4883..7705
                     /locus_tag="ACHKV2_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6942274.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            7782..8741
                     /locus_tag="ACHKV2_004520"
     CDS             7782..8741
                     /locus_tag="ACHKV2_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6942275.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            8746..9060
                     /locus_tag="ACHKV2_004521"
     CDS             8746..9060
                     /locus_tag="ACHKV2_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6942276.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         2498 a         2465 c         2192 g         1936 t
ORIGIN      
        1 taaaaaacgc cctagaaatg acaccgccta ccaaaacccc tacgcagata aagaatatta
       61 tttcttttgg ataaagtcgg attaattctg aagcacgaag tcggacttct ggtaaggtcg
      121 aactctctat gtggcttgat gccgattgtt gctctaacca cgacaatgca gactgtaact
      181 gagaacgagt aagatcgttt aaacgtcctg taccgaaatt gatatggcaa taccgcagaa
      241 gtttttgtcg aagtccacag tcttcactgt tacgtagtaa taaacttaca agagccttac
      301 aggcatcatg atctttacat cgctcaagca ttgcatgcag aaaactctcc gctgttttat
      361 attgatttac tgtcatatca tcaataccag ctacaccaat ctccgcatgt actttttgcc
      421 aaataataaa cgcttcagta ttgctagctt ctgcaatagc agcaaccaag ctatttagct
      481 ccttacgctg agccttaagc aaagggcgat cgtcatcatc attattcgaa gggattgcga
      541 tattgacggt atgagaacca tcatatcgct ctatctgaat attcttttcg tgaaaatcac
      601 gcccagcaac gcgattgttt gaaccgtttg agttgacggc catgtcacct ccctactatc
      661 acctaccctt agtttcgtta tagtcacgac cagcgatacg gttattacca ccagaaatat
      721 ttaactcacg tcctgatggc tgagtttcct tttcactgat cgcacctttt aaagccccaa
      781 tcaccgcgtt tttcacatct aacgaagctg ctcgaaagcg agtaatcaat tcctgctcat
      841 catcgttata agtttcaggt gagtgaattc ccaacacaac atactgaaca tcaaggccaa
      901 aacgagacag cgctgccaaa tacgcagcat caggaaagct atctcctttc tcatatctaa
      961 gctgagttag ctttttgact ccaccaatgt cgctcatggc aacttgacta agtcccaatc
     1021 tttccctttc ctcacgcaac cgccgaccaa tatcattttt catacaaaaa ccttgacagg
     1081 tatctttttt gataccaaaa tgatttcacg agctattaga tgatcacaat ataccactat
     1141 gaaacaagtt cttcacgata ccagatcacg cattccgcgt aacaccgcca caggtccaag
     1201 actggcactt cggctgtccc tcgaggagcg agccgtcatt gatgaaatgg cagctaaaga
     1261 acaacgctca tcctctaaca tggcgcgcat gatctccctt cgcggcctag agctaaccca
     1321 gaaagaacaa aacaaatctt cctgatcagg aggctagtgg gatgtcaggt ataaccatca
     1381 atatcaatgt gaatgccccc tatgtatccc tgcagaaata tgcagagata acaggtatcc
     1441 ctcttaatac atgcaaaaag atgttggctg acggtcgaat tattatccgc cccaaacgcg
     1501 ccaaaatgga aaagcctgaa gtaaaccttg tggcgatgtt aaaagacgct ttggctaaca
     1561 gctaaaacaa tgaacagagc accatcatga aaaaaaacgc taataatcca tactccaaat
     1621 ttcgtaatgg cgtagaacgc catgtacacc acgtcgctac cagtgcatca cgtagtaaca
     1681 gtcgctataa cctgaacgag acgcatgcaa caccggatgg ccacgctgta aaacaaatcg
     1741 gcgaacatgc ctggctgatt gagaaagctg gaatcgtggt ccacaaatgc ccacgcaatc
     1801 cgtttaccgg gaaccgcatt tttgcattga actgcggcga caatcacttc gggcaggatt
     1861 tcacattata cgaagcactt cgcacggttg atcgtctgct tcgcgggcaa agttttatta
     1921 aacaggctga tttataacag gtgctttatg accaaagagc atgcacaagg tgtatttatc
     1981 cgttttattg attttcgcgg tgaactgtta ttacgcgcat cagccattga cggaattgtt
     2041 ccatccgaaa aaaacgcagc tacttacgtt tatctgaacg gtacgcgcct gactgtggag
     2101 cttccgtacc agaccgtacg agaaatcatt agcgaagctg aaaaggctcg tcaggttaat
     2161 ggcgatgaac cctatatcga aattatttgt atggattcag aagctgaaat tcagaaagca
     2221 gattaaaggg cgttgcgatg ggcaaagaat ataaaactct cattaacaaa gcacctgaac
     2281 gattttattt tcgcttaagt gcatcaggcg ctcatgctga acgtgcagcc cgtgactcat
     2341 tgaccagggc aatccggagt ttgtatgacg ttgcttttta cgctgatgat ctggatgcac
     2401 ttaacgaact ttccgagctg atctgtgccg cagaatgcgg ggaacacatt gaaccatata
     2461 agctggggaa tatcgcatga gtatatttat ctcatggctt gttctgatta tttcggtggc
     2521 ctgcgccatt gggattatgc gaattattca ttcagtaaaa aagattgaac gctttttcac
     2581 tgacaaataa cagcttaaac agaacaccag attaaacccg aaaacctgaa aactatccgc
     2641 attcgcggag gtattcgcac acgtaaataa cggagatata aaatgaacgc aaaagaaaaa
     2701 aatattatca atacattaaa aatagtgtca gcagagcaag acaagctgtc cagggcagcc
     2761 cagaaagata atcaacatat ggcagcgctt tacgcactga ccatcgcaat agccacccct
     2821 gaagcagcca aagttattga ggaacagagc aaagaaatcg acactcttaa aacacagtca
     2881 acagttgcag ccatgaatcc gtccagcatt ggacgctgca tttatattct tggttcagca
     2941 atgatgctgc aatacaccat tattgccgaa ctgcacggca aatacctgat aacgccttac
     3001 cacacaaaag agtcagagct tctgacaaat ctccgcctga tagaacgctc tcaagctgta
     3061 ttcattgatg acgcgcaacg tgccgtattt aacgcatagg gttactggac aaagggggcg
     3121 caatggcaat taagcatttt cccgtcgttc gctttacctc cagagggcgc gaatacgagg
     3181 tcgacgaacg cctgattacc actatcgaca aacatcgttc ggaaaaggat gcacaccaca
     3241 tctacctcac tgacggcact tacttctgcg ccaccaacgt ggcgcgagta aatcttatcc
     3301 gacaggtaca ggagccacgc agatgaccat tctggactac atcgctactc atccggggtg
     3361 tagcggcgga gagatcgccg cagcactgaa taccccaacc acagccatta atgctgagtt
     3421 acgccgactt tggcgcggcg gcttagtcat cagaacaaac cgcagcacag gtggtcgcgc
     3481 tcgcaaaaca ggaggccagg cttcttacca cgtaaacccg atgccgttcg ggtgtagcaa
     3541 tccacttact cacatgttta accagctact gaaggaagcc agagcatgag caccatcaac
     3601 caccagaagc tacgcgaact ggcatttgcc ctgcaacgaa tggcaacgcc tcaaaaatta
     3661 ctggcatttc gcgcaatgct ctcgccgtct gctgtgctgg cactgctgga tcagctggag
     3721 cacgccagaa ccacggctcc tgccattcgc ctcacgctcc atcatgaaat cgctgatttc
     3781 tgcgcaacgc tgggggcacc tggcgaaccg gaaacgccgg aagcaatgca gcaagagctg
     3841 ctgcaacgca tcgataacgt ttttgatttt tttctgaacc agtaagaaac cagaacatgc
     3901 acacacaaaa aaaccgcttg ccatgccgca atcggtcagg ttacatttcc gctgcacctc
     3961 ataaaacggg tgccgggatt ctcaacccga tacagagcaa agcgcataac cgcgctagcg
     4021 gtttttttgt gcgtactgta ttgccacgtc tttttcgcgt cagaattatg gcggggcgta
     4081 cggggccgac ttcggtcggg ccggattctt tgctctccgg tgttgagaac cctgtacgtc
     4141 tcgccacccc gagattctca actctggatg gtgagttatt tctatcaccg agcaaagagg
     4201 ccacaccatg gcagaccgca aacagcaccg cgctatcgcg gagcgtcgtc acatccagac
     4261 tgaaatcaac cgcagacttt cccgcgcatc acgcgtcgcg caaatcatgc acatcaatat
     4321 gctgcatgag cgcagccacg cactatcaaa catttattcc gcctctgttt tcagctatct
     4381 ggcggatgat ctgcacgagt ttcaacagct catccagcag caaaacaaac tccattaatt
     4441 cctgttccgg gcctttcctg caccttgcgg cgggaggcct tcgcacatct gtaacaagag
     4501 gattgccgca atgattctcg ccaacgactt tcttgaatac ctgctcaaca cagaacgtga
     4561 tcttgccgct cgcgtgcgtg atcgttatga catgtacctg aaatccctgc ctgtaccgca
     4621 gctcgctgac ggaaagattg ttattgatgg tcgctacatg attgacagcc acgagggaaa
     4681 ttacaggctt taccgcattg aaggtggcac cccgtccgtt attggcattt accagcgccc
     4741 atcctctgca atcgtcgatg tgattgccga cagcatccgc atcacacatc gccatgccga
     4801 cacagaagac accgtgctgg aaattcagcg gctggctaca gcctgccgcg acaccctgaa
     4861 tggcatgacg aagtaaatca ctatgacggc agagtacatc agggactggc aacaaccgcg
     4921 ccacgcagtg gggcgtgaag gaacggggat ccccgctcct gaatccgcgc tttcctcctg
     4981 gctggatgcc taccgggtag agaacgagcg ccgccaggaa atggctgatg cggcgttctc
     5041 cgcaacgccg ctgggcaacc tgattaataa aagcctggac gcacaggaaa aacaggacaa
     5101 aaccatcaca ctggcaggag acgccagaaa acaggcacgc ggtgcggtgg atgaagccat
     5161 ggcctcgctg cgcctgctgc cgtcctatct gcgcgatccg cttattcgcc acctctcctt
     5221 cctgcgcaaa aaacaggaag ccgatcgtca gaaaggcaaa aagagctggc aggctgaacg
     5281 ctacgcgcgc ggaaacctgc gcaaaatatt cgaacgtctg gagcgcaccg atcaccgctg
     5341 gctgacacag ggttatcgct cccttgccgg acgcgaacgc ctggacgatt tgctttacct
     5401 gccgcagctc aacaaacacc agatacagac gctggccacc atgacggcgg cgatgttcag
     5461 cagcaccttc gaaaaactct gcgatggctt tggcgcgacc gatggcgaac tgaccatgga
     5521 tgtaacgctg aaggcgtatc agatgctggc ccgcatggcg ttacacctgc acgccatgcc
     5581 tccacattat gacgcactga caacagacaa agaccggagg aacgaaccgg acacggagct
     5641 gctgccgggc gcaatccttc gcctgacctg tgcggaatgg tggaaacgca aactgtggct
     5701 gttacgttgc gagtggagag aagaacaact ccgcgccgcc tgtctggttt ccagaaaaac
     5761 atcgccctat ctgagccagg acgcgttaag cgagtttcgc gcacagcgcg agaaaacacg
     5821 cgatttcctg aaaagtttca tgctggaaaa cgaagacggg ttcacgattg atctcgagac
     5881 agtgtattac gcgggagtaa gtaacccggt tcaccgtaag gcagaaatga tggccaccat
     5941 gaaggggctg gaacttctgg ccgaagcccg tggcgacaaa gcggtgtttc tgactgtcac
     6001 ctgcccgtca aaataccacg ctacaacaga gaacggtcat ccgaatccca aatggaacgg
     6061 ggccaccatg cgcgactcca gcgattacct ggttaacacg ttttttgcgg cggtccgcaa
     6121 gaaactgaac cgcgacggcc tgcgctggta tggcatccgc acggtggagc ctcaccatga
     6181 cggcaccgtg cactggcata tgatggtctt tgctcatccg gaagaaatcg acaccattgt
     6241 gtcccacacc cgcgatattg ccattcagga agatcgtcac gagctgggcg atgatattac
     6301 tccgcgcttt aaggcggagt atgtcgacgg ctcaaaaggc acgccaacca gctacatcgc
     6361 cacctacatc ggaaaaaacc tggacagccg cgccgtggat ggcatcgacc cgaaaacagg
     6421 caaaccacgc gttgaccacg aaaccggaaa atcaatgacc gagagcgtgg aacgcgccat
     6481 tggctgggcg cgccttcacc gggtccgcca gttccagttc tttggcatcc cctcccgtca
     6541 ggtgtggcgt gaactgcgtc gccttgccag ccagatggca cgcaacccgg aaggcccgca
     6601 acggctgaag gatgacgcaa tggatgcggt tcttgctgcc gctgatgccg gatgttttac
     6661 cacctacatt gagaaacagg gaggcgtact tgttccacgc aaggactacc tgattcgcac
     6721 cgcctacgac ctcgcagatg agctgaacga ttacggcgaa cagagcgtac agatttacgg
     6781 gatctggtca ccactcatcg gggaatcctc ccgtgtgtgc acgcacccgg ataactggaa
     6841 gctggtaaga cgtaaaccgg gagtagaaga cagcgcccgc gaaaatggtt ttgaccttca
     6901 gggcggccct gccgcccctt ggactcgtgg caataactgt ccccgtgtac aggaaacgga
     6961 caacaacggg acagaacagc cggaagaacg gccagcaccg tggccgcagc ttcctgacgg
     7021 cgttgacgtg aacgaatgga tgcgctcact gaaacggcac gaacgccggg cgctgatgcg
     7081 ttcgcttcgt gacaaacagg caaaaaacag cagtgatgaa atgcagagct ggacacagag
     7141 ccgcaaacag cagcggcctt tgcctgataa ccacgaatta ctcgctaaag aatggcggga
     7201 gtctgctgaa tctctcggcc tgcatatcgg tgaacaacag atgcagcacc tgttacgggg
     7261 cggcagtctg tacgttgacg gcagcatcat tgcaccgcag ggatttgaaa ttgtacgcaa
     7321 accggatacc cgcccggaca gccgaatcac gcagctctgg cagcgcctga gccgtaatca
     7381 tggcgtaagc agcacggaga tccgccataa cccggtcgcc agctatctgg cacagctggg
     7441 ggcatcagac cctgaagccg ccgcacgcct ggcatccaca cttcagcagg accagaacac
     7501 catgaaaaca cccgttaccg tgctttctga catgctgcgc gccatccgcg acgcagagca
     7561 cgcacagaga atcagtgaaa ccactgaacg cgccagccgc aaagcagacc tgctgcgggg
     7621 tggcctgacc agtggaaaca aaaaacagac agaaacggga ctcacaaatc ccgtaaatga
     7681 gcaaaaaacg cgccgcgata tatgaagcgc gcacaaaaca ggcaaaagcg ggatttaaaa
     7741 atcctgtaac cgattaatta atcaacataa ggaaaagcga catgaaaatt tgtatcgacg
     7801 acggctccac caacatcaag ctggcatgga ctgagaacgg cgaacgccgc aacgccatca
     7861 gcccgaacag cttcaagtcg gaatggtctg cgccattcgg tggcacgcag cccgcgaact
     7921 acatgcttga tggcgtgcgc tatggttttg atccggtcag cgatcgcttt gtccagacga
     7981 ccgacacgca ataccagtac agcgatgtga atgtaattgc cattcatcac gcgctggtca
     8041 aatcaggcat cacaccacag gaagtggatg tggttgtcac cctgccactg agcgagtatt
     8101 tcgatacaaa cgcacagccg gacatggcca acatcaaccg caaaaaagcg aacgtcatgc
     8161 gcccggtgga gtaccagaac ggcgaagcat tcactatccg taacgtacgg gttatgcctg
     8221 aatccattcc tgctggcttt aaggcactgg ctgacatgag tccgtttgaa tccctgctga
     8281 ttgtggattt gggcggaacc acgctggatg tggcaaaggt tcaggggcaa ctggcaggta
     8341 tcagccaggt gttttgcgat ccacacgtag gcgtttctct gatggccgat gccgtactgt
     8401 cggtgatggc cactaacggt atgcgcacca gtcaccacat cgccaatacc attatcgaac
     8461 atcgccatga tgaagcctgg ctgcgccagc acatccacaa tgacgcgcat tacgccagcc
     8521 tgatggcggt tattcgtgaa aaggaagaaa cactgaaaca acgcgtgatc cgcgcgctgg
     8581 cggttttttc gggttacggg cgggtgatgg ttgtcggtgg cggggcggag attgtggcaa
     8641 ccgctatccg cgaagcctgc ggagttaatg cgactttcat cgcggacggg gtgccacagt
     8701 ttgctctggt taatgggttg tacgcaatga acaaggagta aaccaatgac gacaccaacc
     8761 agacggataa gtttctatct gaagcccgcc gccgtcaaga gtgaacagga ggcgtgcaat
     8821 tacctcgata gcctgccagc ctccgaacgc agccgcgcgc aacgcgcggc ctttctggct
     8881 gggctagcac tcataaagcg caacccagca tttgcctact ggatggccga atggccagag
     8941 gacaacctcc ccttcaatac cgttagcatc aaggaaacat accaggctaa tcagcctagc
     9001 ggagttgaat gcaattttta taaaataaaa aagaatattc agaccttatt cccagaataa
     9061 tctgataatt gtatgtgaac taataaaata c
//
LOCUS       DAZFTN010000161         8863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFTN010000161 DAZFTN010000000
VERSION     DAZFTN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..572)
                     /locus_tag="ACHKV2_004522"
     CDS             complement(<1..572)
                     /locus_tag="ACHKV2_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6942277.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
     gene            complement(666..857)
                     /locus_tag="ACHKV2_004523"
     CDS             complement(666..857)
                     /locus_tag="ACHKV2_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6942278.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(854..1042)
                     /gene="dicB"
                     /locus_tag="ACHKV2_004524"
     CDS             complement(854..1042)
                     /gene="dicB"
                     /locus_tag="ACHKV2_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6942279.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1616..1801
                     /locus_tag="ACHKV2_004525"
     CDS             1616..1801
                     /locus_tag="ACHKV2_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942280.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1988..2377)
                     /locus_tag="ACHKV2_004526"
     CDS             complement(1988..2377)
                     /locus_tag="ACHKV2_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942281.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(2389..2517)
                     /gene="ydfB"
                     /locus_tag="ACHKV2_004527"
     CDS             complement(2389..2517)
                     /gene="ydfB"
                     /locus_tag="ACHKV2_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6942282.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(2519..2674)
                     /locus_tag="ACHKV2_004528"
     CDS             complement(2519..2674)
                     /locus_tag="ACHKV2_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6942283.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2952..3239)
                     /locus_tag="ACHKV2_004529"
     CDS             complement(2952..3239)
                     /locus_tag="ACHKV2_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6942284.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(3239..3430)
                     /locus_tag="ACHKV2_004530"
     CDS             complement(3239..3430)
                     /locus_tag="ACHKV2_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO6942285.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(3458..3859)
                     /locus_tag="ACHKV2_004531"
     CDS             complement(3458..3859)
                     /locus_tag="ACHKV2_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942286.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3968..4240
                     /locus_tag="ACHKV2_004532"
     CDS             3968..4240
                     /locus_tag="ACHKV2_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6942287.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            4224..4649
                     /locus_tag="ACHKV2_004533"
     CDS             4224..4649
                     /locus_tag="ACHKV2_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6942288.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4856..5311)
                     /locus_tag="ACHKV2_004534"
     CDS             complement(4856..5311)
                     /locus_tag="ACHKV2_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942289.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            5390..6481
                     /locus_tag="ACHKV2_004535"
     CDS             5390..6481
                     /locus_tag="ACHKV2_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942290.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            6488..7234
                     /locus_tag="ACHKV2_004536"
     CDS             6488..7234
                     /locus_tag="ACHKV2_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6942291.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            7256..8026
                     /locus_tag="ACHKV2_004537"
     CDS             7256..8026
                     /locus_tag="ACHKV2_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6942292.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            8042..8455
                     /locus_tag="ACHKV2_004538"
     CDS             8042..8455
                     /locus_tag="ACHKV2_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6942293.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2369 a         1897 c         2276 g         2321 t
ORIGIN      
        1 tctgcctgaa tttcaggcca tttagcgcca ggctcacatt tatgcttaac ccacccgatg
       61 gcatgcagtt taagttcagg atacatggcg ttaacttctg gcattttcat caacgcttca
      121 acgatatgtc cgccgaatgt tgccatgtct tcctgtagta attcctgtgc gctaatcacc
      181 atatcaacgg tgatgttttc gcatgtgtcg aatttaacca ggactgcgtt ctgtacttca
      241 ggggacagct tgtcaaaatt gacgttcatc ggatcggatt ctggttcgac cggtacaaag
      301 gaagctgact cctcatccca gcggttttcc tgcatatatt cggtatccca ggaatcgagg
      361 gcagggcggg gtatgccggg tttatcctcg cagacaataa atttataagc gcagtcctga
      421 gctgccggga attgctccag aaattgccag tgaaattttg cgcgtgcgcg acgctcatca
      481 ccggcttcaa tggcagtggc caccgcaaca gcgctatctt cttttatggc ctgttcgtca
      541 ggaatagcag cgcaaataaa gattttactc attgtgtttt aacctcatta cagatttaag
      601 ggtgaacaaa tccctgccat tgctggcata taaaaatgaa accggatatt aattacggtg
      661 ctgttttaaa gtcctgccgg tatttcgtta ttattagtgt gagtagtttt atctaccgga
      721 taacagttac cgggaatttt ttgttctgct gctgcagcca tgcattcttt cattgaaccg
      781 tataagccag tcaccagctc aagagattcg ccggaaacaa gataaactgt cagaacgagt
      841 gcaaatgttg tattcattgt ttatatcctt tttgcagcag gtccagacga gccagcattg
      901 aaggaatgca tacttcattt aacaggtcct gctcgagttt tctctgctta atggcgtctt
      961 caataaatgt tttgtctcca gtgataacgc caatttcgaa acgaagttca gacgtgctgg
     1021 cattacatga taacttttcc attatcgcgt cctcaacaat gaattttgtg atgcggtgcc
     1081 tggtgcctcc aggtgacgtt aaccagttaa caattaacgc cggatacaga gcattctcgt
     1141 tactcagcaa atgaccgctt taccgattta actgttccgc gtgcgctgag ccgcattcac
     1201 cgcatcacaa aattcacttt taaaaaaggg cggcagagca gttacggagt aaaactgata
     1261 ccgccaaatg tcaccagaat attgataaca gagggcgttg cagcggggtt gtcacttaag
     1321 cgtatggtca acctgacaac ccggtgtcct caacggggaa agaataaccc cgccatactt
     1381 accgccgcgc catttcgcgg attgccacaa ccggaagcgc acggtcgaat taaatttaac
     1441 gacggctcta agggagaagg acttcgccgt gcactttcgt gttatgccct gacttttcag
     1501 ggatatatcc tttcagtaaa ctgtcagtgc cggattctta tctgtgtccg gcgcacgacc
     1561 acacgtagca gcgtgttggt ctccattttt aattcagccc ttaatggagg ataaaatgcc
     1621 aaacaaacca tgccctgcct gtaacgctct ttctggcttt ttagagaagg gcggttatta
     1681 catttttaac tgtccagaac atgttgaatt tcatatatca aaacttgaca gtatcattac
     1741 aaagccaaat cagtatcagt ctgatttact gaataaagag ttaaacgccg cccgagatta
     1801 agggcgtagt ggcccattaa aaatattgca aagaccaact attgttgaac tggattaaac
     1861 cagtaattct ctattgctct tattgttacc atcggatgat tgccgggggt tattttaagt
     1921 tcgggaggaa aaggctctcc atcagcagcc atcattgagt atgtgaaatc cgccgacgca
     1981 gtccctttca tgataattcc gaatttttca agtaatgaga gcagttccgg taatcctgtg
     2041 tcatctgaat aactttcctc atagaaagaa gcgattgccg caggagatga cagttctcgc
     2101 tctgcaattt ttttgtagtt ttccagaatt ctttttttga atacttttcc tcgacatgaa
     2161 aacccatcac cgaggcaatc cgctttgcag tttcttcagt attctggttg tttgttaccg
     2221 aaacagagta caatccggta aacgcatcgc gcacattacg agccatatta tcaatgtatt
     2281 tcttcgttgc agattctaat tcaacttcgt tcagacgttg gtgaagtgca tgtgctgcaa
     2341 tttcctggat ttcttttagt aaatctttaa attccatctt taactccgtt agtcgttaga
     2401 ttttgccgaa ctggaaagcg cctgtttaaa ctcactgaag ctgagagctt cctcgccttc
     2461 ggcaaggcct tcgaagtatt cttcgtaagc cttttccatg attgtgtcga aatccatatc
     2521 actcacctga gtttctttcc agccagcgac gggcaccatt ttcggtttta aacgttttgc
     2581 ttttggtata cgtcatcgcg gtgaatgtac cgtcctggtt gggaaacacg ccgtacacca
     2641 gagattcgtt gttgccaaga tcgatagtat ccatgttgac ctcatttccc cttaacgccg
     2701 ggtagcggaa caaaaacctg ctgcatagtt attaaagttg aaccctgccg tcatgttcat
     2761 acgcctcggg ctggctactt accccctgac cactgcttgg taactcgaag tattgcccgg
     2821 cgttctgtgg ggcggggtgg gtggttggtg tatgtaatct acaataaaaa actgtttttg
     2881 tgtcaacagt ttttaattgt tgttttgggc aaaaaaatcc ctcgaaagag ggagtatgaa
     2941 aattgttcaa ctcagataga gaagggaaat tgtcgccgag aatgtgtcac gcttacaatc
     3001 tcaatgcttg aagcagctac tctgtacagg attatgtagt tagggtgggt cacgatctct
     3061 ctcaatccag aaacccgttc gcttggtgga tataagtacg gatgctcaga tagaggtaat
     3121 accgatgttt caatgcgtat ttttagtcta cgtgctgccg gtgggttctc cttggcgatg
     3181 taagttatga tctggcgcaa atcatcgcga gcagacggta gccataaaat gggtaacatt
     3241 actcgctctt gttagttaca gcaatttgag caataagatt ttccatttca gccattacct
     3301 cgtcatgtgg aattgcgggg cgagtgtctg caaggcttga cgttacttta gtgcgcaacc
     3361 attcgttgta actgttttct tgttcggtag tttcgaattc tgaaactatc ggagaaaggg
     3421 ctgtacccat ggcataactc ctcttcttgt actgtggtca cgcccggcgg cttttttgtg
     3481 ccgccagcca cctagcaatg gtttcttcca ttgatttttt cttgtctttg atttcttgaa
     3541 gcattttttc ttggtcttcc tcaggaaacg cactaaaagc ttggagcagt tcgcgttggc
     3601 gaggaccaat tttcatcgtg tcaggggtga aaatttgctc acactcttca ggaggcaata
     3661 aaaaccaatg caatggatgc cctgaaacct caaccagttt atccaaactt gaggctttag
     3721 gtgtagcctt accgctgacc cattgttgaa cagtttgttg tgtcacacca attctacggg
     3781 caagctcagc ctggctccat ccagtttcct gaagaagctt gctgattctg tacatagata
     3841 cttctagggc gctcatcatt attcaatttt acaggtaaat actgttaaaa gcatcacaat
     3901 aaaaaactgt tgattgcata cagtttttta ttgtaggctt tgcttatagt ttttagagga
     3961 gggcaaaatg ctagatagca ctcgcgaaaa aattaggcag aaatacactc aggctgaaat
     4021 aggtcgttat atgggggtcg ctcaacagac tgtttggcaa tggtttagct ttggcgttcc
     4081 cccaaagcag gtaattccgt tatgccaact aatgaagtgg gaagttaccc cgcatgaaat
     4141 ccgcccagat atttatccta acccaaccga cggtttacct gttggatgta aggttaacac
     4201 atcaaatgcg ccggagttga ttcatgaaaa tcaagcatga acacatccgc atggcgatga
     4261 atgcctgggc gcatccggac ggcgaaaaag taccggctgc gaaaattacc aaagcgtatt
     4321 tcgagctggg aatgacgttc ccggaactgt atgacgacag ccatccggaa gccatggctc
     4381 gcaatactca gaaaattttc cgctgggtgg agaaagacac tcctgatgcg gttaaaaaaa
     4441 ttcaggcgtt gttaccagcg atcgaaaaag cgatgccgcc tccgctggtg gcccgaatgc
     4501 gcagccacag ttccgcttat tttcgggagt tggtagagac gaaggaacgg ctggtgaaag
     4561 atattgatga tttcgttgca tcagcgatcg ttctgttcga tcagatgaat cgtggtggcc
     4621 cggcaggaaa cactctggct gtgcattaat tgggtaataa atatgagtaa tgacaaaaaa
     4681 ttgacactga gcgtttacga aaacagtccg cacatctggc gtggcggttt atctgatgtg
     4741 gagctggcag agtggttgat acataaagct aatgcgctgc tctggcgttt gtcagccaga
     4801 gaacagcgca aggaaaccag aataaagctg gctgatgcag aagcgtgtgc cgggcttatt
     4861 gaggattata caaatcttgg tatttcttca gcagagagtg atcccattca gcctctgagc
     4921 agggagtcaa tccagcacgc tggttgtatg gcacatcttg taactgctcg tcaacatgag
     4981 gtgggtattg gatcacttcc ggcgggatat tcgctgattc cagagctggt tgaagcaaga
     5041 aaatcagttc agaaaaagag agatgacgca cttcaattat tgagagagca ctatggcgcg
     5101 ataccagaat gcgaacagcg tcgataccct gaaggttatg aatggatgca gtctcttttt
     5161 gaagttcgct aatcaatatg tcgagacgaa ggtatgtttc ggcgcgcagc caggctctgt
     5221 aatccgggag catttcgggg ctgttacacc agcggtttgt tgctgcaaca tttaatacat
     5281 gagcctgata aaggcttttc aaaaaataca tgtcgaacct cctctggttc tgtcgattgg
     5341 gaaccacaga ttatatccgg aggaaggttc ggcaccagat gaggtagcca tgcgtgatta
     5401 cgcaaaagtt tctccgcgat tctggctggg agaaacgggg agagaactta gaaaggcggg
     5461 tgcagaagcg caagttgttg ctttttacct gatgacatcc cctcacgcaa atatgctggg
     5521 tttgtattac ctgccagttt tataccttgc tcatgaaacc gggcttggtc tggaaggggc
     5581 ttcaaagggg cttaaaaggg ctgttgaagc tggtttttgt agctatgacc atgatgcaga
     5641 gatggtctgg gtccatgaaa tggcagcctg gcaggttggg gaaacgttga agcctggcga
     5701 taaccgttgt gcaggtgtca ggaatgagta tgcatcatta cctgaaaacg cttttctgtc
     5761 agtgttttac gacagatata aaacggattt ccatctggat gtgaggcgga ataatagccg
     5821 aaattcggta aggggcttcg aaggggcttt taaggggctt cgaagccaag aacaggaaca
     5881 ggagcaggag aaagaacagg aacaggacaa aaacactatg gttcatggca aaaaaaacac
     5941 cacgaaccag gcaggggatg ttcagaccgt caatcctggt cagccagcag gcacgacacc
     6001 ggaagccgat tcgggcgctg tgcagcaggt gatgaccgca gggtcggagc aatcacacca
     6061 actgcagcag cctgaagccg attccgccat tcagcgggaa gccgatcggg tagtcccgga
     6121 aagcaccggg cagtctgtgg gacgagtgga ttatccggat gtgttcgaac aggtctggcg
     6181 ggaatacccg ttgcgtgctg gggcaaaccc gaagaaatcc gctttcagtg cctggaaggc
     6241 cagattgcgc gagggggtgc caccagagac catgctggat ggtgtgaggc gttacgcgag
     6301 atacctggcg gcgaccggga aagcgggaac ggaatttgtt cagcgagcga cgacgttttt
     6361 tgggccggac cggaattttg aaaacccctg gttgctcccg gtaagcggca cgaacaacca
     6421 gcgttgtgtg aatcatattt ctgaaccgga taccgaaatt ccgccgggat tcagggggtg
     6481 atgtggcatg aaaaacattg cggcagccgg ggttcttgaa cgtattcgca gacttgcacc
     6541 acaggcgtcg gttccaccgt accggacggt ggaggagtgg cgggaatggc aacttgctga
     6601 aggacgaaaa cgcagcgagg agattaaccg ccagaatcac cagttgcggg tggaaaaaat
     6661 cctgaatcgt tcgggcatcc agcctctgca cagcaaatgc tcgtttgcga attatcaggt
     6721 gcagaacgac gggcaaaaat acgcgctgag ccaggccaaa tccatagctg acgaactgat
     6781 gaccgggtgc acgaattttg tgttcagcgg taaaaccggc accgggaaaa atcaccttgc
     6841 agcggcgatg ggtaaccggc tgatggcgaa ggggcgcagc gtgattatcg tcaccgtgtc
     6901 tgatgtcatg agcgtgttgc atgacagcta cgacaacggc aaatccgggg aaaaattttt
     6961 acaggagctt tgcagtgttg atttgctggt cctggatgaa ataggcgttc agcgggagac
     7021 gaaaaacgag caggtggtat tacaccagat aattgatcgc cggacagcat cactgtgcag
     7081 tgtcgggatg ttaacaaacc tgaatcatgc cgcaatgagt acacttcttg gtgagaggat
     7141 tatggaccgc atgaccatga acggtggtcg atgggtgacg tttaactggg atagctggcg
     7201 tccaaatgtc agcaatatga gggttgtgaa gtaattttgt ccggaggaaa ttttaatgga
     7261 aaccgtatct gacgcactga aagcactgaa aaaagcctct tcacatgtgg tggcagctcg
     7321 ccttggaatc agtcgtgaag aggctgtcaa cgagctgtgg gaactcaaaa taaatggcgt
     7381 cgttgataaa actggtcaca cctggtttct ggctggcgaa ggtgaatccc gggtaaccga
     7441 agagcggcca gtaaaatctg aagcacagga tatgctgacc ggggaggtcg aacaaaaagt
     7501 taccgcagac atgatgattg agtttatcgg tcaggatggg gctaaaacgt gtgaggaact
     7561 ggcgggtaag ttcggtgtca gtactcgcaa ggttgcttcc acgctggcgg tggtaaccgc
     7621 aacggggcgg ctggcacgcg ttaatcagaa cggtaaattt cgttactgca tgccgggcga
     7681 taatttacca gcagagccga aagccgcgct ggtaacggaa agtgatggta aggcctttcc
     7741 tcagccagca ggtgctgcgt taccagtccg ggaagccgca acacaggaag aaattaaaac
     7801 agaaactgtg gcggacattg tgcagccgtt gccatcgttt accgaaacgc aagcagatga
     7861 gctgattttt ccgtcccttc gcagggcaaa cctggcgctg cgcagggcga aaagtgatgt
     7921 tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagcacc gggatattgt
     7981 tcgacagatt actgattctt cccgccgtgt tgtatcggaa aagtgattgc cggaggcgct
     8041 tatggcaaaa gtatttacac aggaagagcg ggaaaaaatt aaagggcagg ttgttgagct
     8101 agtacgccgg agtgggcgcg agacgttacg gcaactggaa gccaagacag gtgcgacaag
     8161 atatctgatg agcgttcttg ccagagagct ggttgccagt ggcgatgtat acaactctgg
     8221 ttacgggtta ttcccatctg aacaggctcg taaggactgg caaaacgccc gcaaaaaact
     8281 atcgagggca aaactgaaga aaccggttgt ggttgatcct gaccttatct ggtcattacc
     8341 agacggagaa atacgccgct atgacaggca attgaacata atttgtagtg agtgccgtaa
     8401 cagtgaagtg atgcagcgag ttttgatatt ttacacagga gtaatgatgg aatagtgaaa
     8461 ataacatgaa acctttggat tctggtgttt cagtggatgg gaaaagagca gatacataga
     8521 aaatgaatag caataattca cagtctggat gttgtttgta tgcttataaa atgatcctag
     8581 tatgcattgc cggagttgaa tcagctccaa tgaagaaact gagaggagta tttagaagat
     8641 agctaaatgg atgtaattat tagtatctaa aaaatagtat ttttgaaatg ggtctaagaa
     8701 cacaacaatg tacaaatgag aagttatctt ttaatatgtt caaagaccca ttaggattga
     8761 tatcagttat gttagctaga ttgataaagt gattacatat tttctgttat atgctgaaat
     8821 gaataatgct attccagagt gcagcagaga aagtctcctg atg
//
LOCUS       DAZFTN010000162         8642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFTN010000162 DAZFTN010000000
VERSION     DAZFTN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(272..535)
                     /gene="rpsT"
                     /locus_tag="ACHKV2_004539"
     CDS             complement(272..535)
                     /gene="rpsT"
                     /locus_tag="ACHKV2_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO6942294.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            638..862
                     /locus_tag="ACHKV2_004540"
     CDS             638..862
                     /locus_tag="ACHKV2_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO6942295.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            870..1811
                     /gene="ribF"
                     /locus_tag="ACHKV2_004541"
     CDS             870..1811
                     /gene="ribF"
                     /locus_tag="ACHKV2_004541"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO6942296.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1854..4670
                     /gene="ileS"
                     /locus_tag="ACHKV2_004542"
     CDS             1854..4670
                     /gene="ileS"
                     /locus_tag="ACHKV2_004542"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO6942297.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4670..5164
                     /gene="lspA"
                     /locus_tag="ACHKV2_004543"
     CDS             4670..5164
                     /gene="lspA"
                     /locus_tag="ACHKV2_004543"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO6942298.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDMVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5252..5701
                     /gene="fkpB"
                     /locus_tag="ACHKV2_004544"
     CDS             5252..5701
                     /gene="fkpB"
                     /locus_tag="ACHKV2_004544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6942299.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5703..6653
                     /gene="ispH"
                     /locus_tag="ACHKV2_004545"
                     /gene_synonym="lytB"
     CDS             5703..6653
                     /gene="ispH"
                     /locus_tag="ACHKV2_004545"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO6942300.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6719..7633
                     /gene="rihC"
                     /locus_tag="ACHKV2_004546"
     CDS             6719..7633
                     /gene="rihC"
                     /locus_tag="ACHKV2_004546"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO6942301.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7800..8621
                     /gene="dapB"
                     /locus_tag="ACHKV2_004547"
     CDS             7800..8621
                     /gene="dapB"
                     /locus_tag="ACHKV2_004547"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO6942302.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1969 a         2165 c         2450 g         2058 t
ORIGIN      
        1 tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt ctttgtaggg
       61 gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg ttctacgatt
      121 cttaagccac gaagagttca gatagtgcaa cggcatgtct cttttgacta tctggcaacc
      181 ggcagtgtgt tctctcacgc atcacaaaag cagcaggcat aaaaaaaccc gcttgcgcgg
      241 gctttttcac aaagtttcag caaattggca attaagccag tttgttgatc tgtgcagtca
      301 ggttagcctt atgacgtgca gctttgtttt tgtggatcag acctttagca gcctgacggt
      361 ccacgatcgg ttgcatttcg ttaaatgctt tctgtgcagc agctttgtcg ccagcttcga
      421 tagctgcgta tactttcttg atgaaagtac gcatcataga gcgacggctt gcgttgtgct
      481 tacgagcctt ttcagactga atggcgcgct tcttagctga tttgatatta gccaaggtcc
      541 aactcccaaa tgtgttctat atggacaatt caaaggccga ggaatatgcc cttttagcct
      601 tcttttgtca atggatttgt gcaaataagc gccgttaatg tgccggcact cgttacgtag
      661 tgatggcgca ggattctacc agcttgcggg gtgtgaatac agcttttccg cgataaaaat
      721 tgcagcaggc agtcagtttc ttcccgtgat ttgcgccatg accatgacaa tgaaaagcca
      781 cttctttctg atttcggtac tcaatcgccg gttaaccttg accgctgtac aaggtatact
      841 cggacgattt tcactgtttt gagccagaca tgaagctgat acgcggcata cataatctca
      901 gccaggcccc gcaagaaggg tgtgtgctga ctattggtaa tttcgacggc gtgcatcgcg
      961 gtcatcgcgc gctgttacag ggcttgcagg aagaagggcg caagcgcaac ttaccggtga
     1021 tggtgatgct ttttgaacct caaccactgg aactgtttgc taccgataaa gccccggcac
     1081 ggctgacccg actgcgggaa aaactacgtt accttgcaga gtgtggcgtt gattacgtgc
     1141 tgtgcgtacg tttcgacagg cgtttcgcgg cgttaaccgc gcaaaatttc atcagcgatc
     1201 ttctggtgaa gcatttgcgg gtaaaattcc tcgccgtggg ggatgatttc cgctttggcg
     1261 ctggtcggga aggcgatttc ttgttattac agaaagctgg catggaatac ggcttcgata
     1321 tcaccagtac gcaaactttt tgcgaaggtg gcgtgcgtat cagcagcacc gccgtgcgtc
     1381 aggcccttgc ggatgacaat ttggctctgg cagaaagttt actggggcac ccgtttgcca
     1441 tctccgggcg tgtagtccac ggtgatgaat tagggcgcac tataggtttc ccgacggcga
     1501 atgtaccgct acgccgtcag gtttccccgg tgaaaggggt ttatgcggta gaagtgctgg
     1561 gcctcggtga aaagccgtta cccggcgtgg caaacatcgg aacacgccca acggttgccg
     1621 gtattcgcca gcagctggaa gtgcatttgt tagatgttgc aatggacctt tacggtcgcc
     1681 atatacaagt agtgctgcgt aaaaaaatac gcaatgagca gcgatttgct tcgctggacg
     1741 aactgaaagc gcagattgcg cgtgatgaat taaccgcccg cgaatttttt gggctaacaa
     1801 aaccggctta agcctgttat gtaatcaaac cgaaacacgg aaccgagaat ctgatgagtg
     1861 actataaatc aaccctgaat ttgccggaaa cagggttccc gatgcgtggc gatctcgcca
     1921 agcgcgaacc gggaatgctg gcgcgttgga ctgatgatga tctgtacggc atcattcgtg
     1981 cggctaaaaa aggcaaaaaa accttcattc tgcatgatgg ccctccttat gcgaatggca
     2041 gcattcatat tggtcactcg gttaacaaga ttctgaaaga cattatcgtg aagtccaaag
     2101 ggctttccgg ttatgactcg ccgtatgtgc ctggctggga ctgccacggt ctgccgatcg
     2161 agctgaaagt agagcaagaa tacggtaagc cggatgagaa attcaccgcc gccgagttcc
     2221 gcgccaagtg ccgcgaatac gcggcgaccc aggttgacgg tcaacgcaaa gactttatcc
     2281 gtctgggcgt gctgggcgac tggtcgcacc cgtacctgac catggacttc aaaactgaag
     2341 ccaatatcat ccgcgcgctg ggcaaaatca tcggcaatgg tcacctgcac aaaggcgcga
     2401 agccggtgca ctggtgcgta gactgccgtt ctgcactggc agaagcggaa gttgagtatt
     2461 acgacaaaac ttctccgtcc attgacgtcg ctttccaggc ggtcgatcag gatgcgctga
     2521 aagcgaaatt tggcgtaagc aacgttaacg gcccaatctc gctggtgatc tggaccacta
     2581 cgccgtggac tctgcctgcg aaccgcgcaa tctctattgc acctgatttc gactatgcgc
     2641 tggtgcagat cgacggtcag gccgtgattc tggcgaaaga tctggttgaa agcgtaatgc
     2701 agcgtatcgg cgtgaccgat tacaccattc tcggcacggt aaaaggtgcg gagcttgagc
     2761 tgctgcgctt tgcccatccg tttatgggct tcgacgtccc ggcaatcctc ggcgatcacg
     2821 ttaccctgga tgccggtacc ggtgccgttc acaccgcgcc tggccacggc ccggacgact
     2881 atgtgatcgg tcagaaatac ggcctggaaa ccgctaaccc ggttggcccg gacggcactt
     2941 atctgccggg cacttatccg acgctggatg gcgtgaacgt cttcaaagcg aacgacatcg
     3001 tcgttgcgct gctgcaggaa aaaggcgcgc tgctgcacgt tgagaaaatg cagcacagct
     3061 atccgtgctg ctggcgtcac aaaacgccga tcatcttccg cgcaacgccg cagtggttcg
     3121 tcagtatgga tcagaaaggt ctgcgcgcgc agtctctgaa agagatcaaa ggtgtgcagt
     3181 ggatcccgga ctggggccag gcgcgtatcg agtcgatggt cgctaaccgt cctgactggt
     3241 gtatctcccg tcagcgtacc tggggcgtac cgatgtctct gttcgtgcac aaagacacgg
     3301 aagagctgca tccgcgtacc ctcgaactga tggaagaagt ggctaaacgc gttgaagttg
     3361 atggcatcca ggcgtggtgg gatcttgatg cgaaagagat cctcggcgat gaagctgatc
     3421 agtatgtgaa agtgccggat acgctggatg tatggtttga ctccggctct actcactctt
     3481 ctgttgttga cgtgcgcccg gaatttgccg gtcacgctgc ggacatgtat ctggaaggtt
     3541 ctgaccaaca ccgtggctgg ttcatgtctt ctctgatgat ctccaccgcg atgaagggca
     3601 aagcgccgta tcgtcaggtg ctgacccacg gctttaccgt agatggtcag ggccgcaaga
     3661 tgtctaaatc catcggcaac accgtgtcgc cgcaggatgt gatgaacaaa ctgggcgcgg
     3721 atattctgcg tctgtgggtg gcatcaaccg actacaccgg tgaaatggcc gtttctgacg
     3781 agatcctgaa acgtgctgcc gacagctatc gtcgtatccg taacaccgcg cgcttcctgc
     3841 tggcaaacct gaacggtttt gatccggcaa aagatatggt gaaaccggaa gagatggtag
     3901 tactggatcg ctgggccgta ggttgtgcga aagcggcaca ggaagacatc ctcaaggcat
     3961 acgaagcata cgatttccac gaagtggtac agcgtctgat gcgcttctgc tccgttgaga
     4021 tgggttcctt ctacctcgac atcatcaaag accgtcagta caccgccaaa gcggacagcg
     4081 tggcgcgtcg tagctgccag actgcgctgt atcacatcgc agaagcgctg gttcgctgga
     4141 tggcaccaat cctctccttc accgctgatg aagtgtgggg ctacctgccg ggcgaacgtg
     4201 aaaaatacgt cttcaccggc gagtggtacg aaggtctgtt tggtctggca gacagtgaag
     4261 caatgaacga tgcgttctgg gacgagctgt tgaaagtgcg tggcgaagtg aacaaagtca
     4321 ttgagcaggc gcgtgccgac aagaaagtgg gtggctcgct ggaagcggca gtaaccttgt
     4381 atacagaacc ggaactggcg gcgaaactga ccgcattggg cgatgaatta cgatttgtcc
     4441 tgttgacctc cggcgctacc gttgcagact ataacgacgc acctgctgat gctcagcaga
     4501 gcgaagtgct caaagggctg aaagtcgcgt tgagtaaagc cgaaggtgag aagtgcccac
     4561 gctgctggca ctacacccag gatgtcggca aggtggcgga acacgcagaa atctgcggcc
     4621 gctgtgtcag caacgtcgcc ggtgacggtg aaaaacgtaa gtttgcctga tgagtcaatc
     4681 gatctgttca acagggctac gctggctgtg gctggtggta gtcgtgctga ttatcgatct
     4741 gggcagcaaa tacctgatcc tccagaactt tgctctgggg gatatggtcc cgctgttccc
     4801 gtcgcttaat ctgcattatg cgcgtaacta tggcgcggcg tttagtttcc ttgccgatag
     4861 cggcggctgg cagcgttggt tctttgccgg tattgcgatt ggtattagcg ttaccctggt
     4921 tgtgatgatg tatcgctcga aggccacgca gaagctaaac aatatcgcct acgcgctgat
     4981 tattggcggc gcgctgggca acctgttcga ccgcctgtgg cacggcttcg ttgtcgatat
     5041 gatcgacttc tacgtcggcg actggcactt cgccaccttc aacctcgccg atactgccat
     5101 ctgtgttggt gcggcactga ttgtgctgga aggttttttg ccttctaaag cgaaaaaaca
     5161 ataataaacc ctgccggata aggcctacag attgctgcga aatcgtaggc cgcatccggc
     5221 aaaaatcctc aaaatataag agcaaacctg catgtctgaa tctgtacaga gcaatagcgc
     5281 cgtcctggtg cacttcacgc taaaactcga cgatggcacc accgccgagt ctacccgcaa
     5341 caacggtaaa ccggcgctgt tccgcctggg cgatgcttct ctttccgaag ggctggagca
     5401 acacctgctg gggctgaaag tgggcgataa aaccactttc tcgctggagc cagatgcggc
     5461 gtttggcgtg ccgtcaccgg acctgattca gtacttctcc cgccgtgagt ttatggatgc
     5521 aggcgagcca gaaattggcg caataatgct ttttaccgca atggatggca gtgagatgcc
     5581 tggcgtgatc cgcgaaatta acggcgactc cattaccgtc gatttcaacc atccgctggc
     5641 cgggcagacc gttcattttg atattgaagt gctggaaatc gatccggcac tggaggcgta
     5701 acatgcagat cctgttggcc aacccacgtg gtttttgtgc cggggtagac cgcgctatca
     5761 gcattgttga aaacgcgctt gccatttacg gcgcaccgat atatgtccgt cacgaagtgg
     5821 tgcataaccg ctacgtggtc gatagcctgc gcgagcgtgg agctatcttt attgagcaga
     5881 tcagcgaagt gccggacggc gcgatcctga tcttctccgc acatggtgtt tctcaggcgg
     5941 tacgtaacga agcgaaaagc cgtgatttga cggtattcga cgccacctgt ccgctggtga
     6001 ccaaagtgca tatggaagtc gcccgcgcca gccgtcgtgg cgaagagtct attctcatcg
     6061 gtcacgccgg gcacccggaa gtggaaggga cgatggggca gtacagcaac cctgaagggg
     6121 gaatgtatct ggtcgaatcg cctgacgatg tgtggaaact gacggtcaaa aacgaagaga
     6181 agctctcctt tatgacccaa accacgctgt cggtagatga cacgtctgat gtgatcgacg
     6241 cgctgcgtaa acgcttcccg aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca
     6301 cgactaaccg tcaggaagcg gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg
     6361 tcggttcgaa aaactcctcc aactccaacc gtctggcgga gctggcccag cgtatgggca
     6421 aacgcgcgtt tttgattgac gatgcgaaag atatccagga agagtgggtg aaagaggtta
     6481 aatgcgtcgg cgtgactgcg ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg
     6541 cacgtttgca gcagctgggt ggtggtgaag ccattccgct ggaaggccgt gaagaaaata
     6601 ttgttttcga agtgccgaaa gagctgcgtg tcgatattcg tgaagtcgat taagtcatta
     6661 gcagcctaag ttatgcgaaa atgccggtct tgttatcggc attttttatg gagaaaacat
     6721 gcgcttacct atcttcctcg atactgaccc cggcattgac gatgccgtcg ccattgccgc
     6781 cgcgattttt gcccccgaac tcgacctgca actgatgacc accgtcgcgg gtaatgtctc
     6841 ggttgagaaa actacccgta acgccttgca actgctgcat ttctggaatg cggagattcc
     6901 gctcgcccag ggagccgctg tgccgctggt acgcgcaccg cgtgatgcag catctgtgca
     6961 cggcgaatcg ggaatggctg gctacgactt tgttgagcac aaccgaaagc cactcgggat
     7021 accggcgttt ctggcgattc gggatgccct gatgcgtgca ccagagcccg ttaccctggt
     7081 ggccatcggc ccgttaacca atattgcgct gttactttca caatgcccgg aatgtaaacc
     7141 gtatattcgc cgtctggtga tcatgggtgg ttctgccgga cgcggcaact gtacgccaaa
     7201 cgccgagttt aatattgctg ccgatccaga agctgctgcc tgtgtcttcc gcagtggtat
     7261 tgaaatcgtc atgtgcggtt tggatgtcac caatcaggca atattaactc ctgactatct
     7321 ctctacactg ccgcagttaa accgtaccgg gaaaatgctt cacgccctgt ttagccacta
     7381 ccgtagcggc agtatgcaaa gcggcttgcg aatgcacgat ctctgcgcca tcgcctggct
     7441 ggtgcgcccg gacctgttca ctctcaaacc ctgttttgtg gcagtggaaa ctcaaggcga
     7501 atttacctca ggcacgacgg tggttgatat cgacggttgc ctgggcaaac cagccaatgt
     7561 acaggtggca ttggatctga atgtaaaagg cttccaacag tgggtggctg aggtgctggc
     7621 tctggtgccg taacctgtca catgttatcg gcatgcagtc attcatcgac tcatgccttt
     7681 cactgatatc cctccctgtt tatcattaat ttctaattat cagcgttttt ggctggcggc
     7741 gtagcgatgc gctggttact ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta
     7801 tgcatgatgc aaacatccgc gttgccatcg cgggagccgg ggggcgtatg ggccgccagt
     7861 tgattcaggc ggcactggca ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag
     7921 gatcttcttt actgggcagc gacgccggtg agctggctgg tgcagggaaa acaggcgtta
     7981 ccgtgcaaag cagtcttgat gcgataaaag atgattttga tgtgtttatc gattttaccc
     8041 gtccggaagg tacgctgaac catctcgctt tttgtcgcca gcatggcaaa gggatggtga
     8101 tcggcactac ggggtttgac gaagccggaa aacaggcaat tcgtgacgcc gccgccgata
     8161 ttgcgattgt ctttgccgcc aattttagcg ttggcgttaa cgtcatgctt aagctgctgg
     8221 agaaagcagc caaagtgatg ggtgactaca ccgatattga aattattgaa gcacatcata
     8281 gacataaagt tgatgcgccg tcaggcaccg cactggcaat gggagaggcg atcgcccacg
     8341 cccttgataa agatctgaaa gattgcgcgg tctacagtcg tgaaggccat accggtgaac
     8401 gtgtgcctgg taccattggt tttgccaccg tgcgtgcagg tgacatcgtt ggcgaacata
     8461 ccgcgatgtt tgccgatatt ggcgagcgtc tggagatcac ccataaggcg tccagccgca
     8521 tgacatttgc taacggtgcg gtaagatcgg ctttgtggtt gagtggtaag gaaaatggtc
     8581 tttttgatat gcgagatgtg cttgatctca ataatttgta accacaaaat gtttatgatg
     8641 at
//
LOCUS       DAZFTN010000163         8636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFTN010000163 DAZFTN010000000
VERSION     DAZFTN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..236)
                     /locus_tag="ACHKV2_004548"
     CDS             complement(<1..236)
                     /locus_tag="ACHKV2_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6942303.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWYSAAIFLIPICISFDT"
     gene            complement(302..796)
                     /locus_tag="ACHKV2_004549"
     CDS             complement(302..796)
                     /locus_tag="ACHKV2_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6942304.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            complement(796..1203)
                     /locus_tag="ACHKV2_004550"
     CDS             complement(796..1203)
                     /locus_tag="ACHKV2_004550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO6942305.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(1213..2019)
                     /locus_tag="ACHKV2_004551"
     CDS             complement(1213..2019)
                     /locus_tag="ACHKV2_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6942306.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(2089..3036)
                     /locus_tag="ACHKV2_004552"
     CDS             complement(2089..3036)
                     /locus_tag="ACHKV2_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6942307.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            3384..4256
                     /gene="rluF"
                     /locus_tag="ACHKV2_004553"
     CDS             3384..4256
                     /gene="rluF"
                     /locus_tag="ACHKV2_004553"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO6942308.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(4257..4529)
                     /locus_tag="ACHKV2_004554"
     CDS             complement(4257..4529)
                     /locus_tag="ACHKV2_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO6942309.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4782..6131)
                     /gene="lysC"
                     /locus_tag="ACHKV2_004555"
     CDS             complement(4782..6131)
                     /gene="lysC"
                     /locus_tag="ACHKV2_004555"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO6942310.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(6223..6419)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6656..8305
                     /gene="pgi"
                     /locus_tag="ACHKV2_004556"
     CDS             6656..8305
                     /gene="pgi"
                     /locus_tag="ACHKV2_004556"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO6942311.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         2159 a         2220 c         2075 g         2182 t
ORIGIN      
        1 gtatcaaatg aaatgcaaat cggtatcaaa aaaatcgccg cgctatacca gccgagagct
       61 atcaattcca gcgtaccacc gagtattatt ccggttttta aatcaccgag aattaaacca
      121 atcaccgtac aggcaattaa cgggcgatga gtctgaaatt catccagcac gctgcccatt
      181 ccggcaatac aggaaaaaag aaatatagca atgatttgta gggtacttat ttccatgatt
      241 tgtcaccttt taagtgtcgt catccctgcc tgttaaagca tatgtacaca ggcgctatta
      301 tttaatttgc gattaactgt tcgtttattt tgtagataat attgattgag ggatctgacg
      361 ctacgacgcg taaatccagt atgacgccta gattatttaa ctcatgaaat gcgttaatat
      421 catcatcatc taatgaaacc gctttggtta actgtttttt accgggccgc caggccatgc
      481 cgccaatatt cagggtgcct atttttaccc cctggcgaac catcgctaag gcatcctgtg
      541 ggcgagtgaa aagataaaaa acggtttcgt cctgatattg cgggttgtga taaaccgcga
      601 cggctttttc aatattgacc acgttaacct tcattcccgg tggcgcagcc tgacgcaata
      661 atgtccggcg aacttcatcg ttataaactt cgtcattgca gataataatg cgctgggcgt
      721 tagccacttt cgaccagacg gtagtgacct gaccgtggat caggcggtca tcaatgcggg
      781 cgagcgtaat gttcatcaga aatcctcctc tgtcgttgcc agttgtttcc agacaacgca
      841 ggtttgcttt gcgatttgcg tcaggtgttc acacagttca tcaacattca tgctgtcctg
      901 gttatcaacc agttcgagcg ccagcggcag agaaagtccg ctgattaccc gcagacgagg
      961 attgcgcatc gcaagcatcg ctgccgcatt ccacgggctg ccgcactgta aatccacggc
     1021 aatcagccac tcattttgat tgtgaatgct tactaatttt tccagctttg ctacgatgtc
     1081 tccggcgttt tcaccgggca caaattcaac ggcttcaaca ttggcgttgc cgtaaaccat
     1141 ctgaaccgat tccagcatgg cgcaagctaa tttgccgtgg gcacaaaaaa tggcgttgac
     1201 cataaagcct ccttagccgc gcgttttgcc gcccgcaatg ttagtggtta ctccggtgat
     1261 atagctggcg cgttcagaca gcagataaca aacaaaatca gcgatttctg ctaatcttcc
     1321 ggcgcgccca ataggaatgg cgtttttggt atagccttca cgcagctgct cgacggtgat
     1381 attgcgcgtc cacgccagcg cctcttcata ttccggcgtg cgtagcccgg ttttttccag
     1441 aatccccggc gcgataccga ccacacggat accgtgttta cccagctctt tcgaccagga
     1501 gcgcgtgaag ctattgagcg cggctttggt agcggcgtaa cagctttggc cttctgagcc
     1561 ttccagccca ctttctgagg aaacattcac aatcacgcca tcatgttgtt tgaccatctg
     1621 tcgcgccacc gcctgcgaca tcagaaaaac gcctttctgg ttgatattga ccattttttc
     1681 gaatgcagct tcgttgagtt catactgccc ggcaggcgct ttctcatcga ccagcagacg
     1741 cgggaaattg accccggcgt tattgaccag accgtcgatg cgaccaaaac gctggataat
     1801 ttccgctacc gtatgattta cctctttggc gctggaaata tcggtcggcc aaaactgata
     1861 gcctttatga ccttcatatt gtccatcgcc accgtgaata tctaccatct gtacgtttgc
     1921 gccttgtgct aataattcct ctacaatcgc cagaccaata ccggatgcgc cgccggtgac
     1981 gataatgatt ttatcctgca aatttaacca cgtttgcata acagactcct gattttagat
     2041 aatagggacc cgccgtaatt tttattacga taaacatcgg gaagattttt atttcagtaa
     2101 tagttcagct gtgctgctat tcgttaccag acaattaata tattttccac gcagtgcacc
     2161 aataattccg ctgtattttt cttcactcat ggcaatgcca atggaatatc gtgcctgctt
     2221 taatttattc atttcgatag agagtgtttt ttcgctcata ttcgtttcaa ccatttcgcc
     2281 gtgaatatca aaaaagcgcg agcaaatatc gccagcaacc tggcgggcat tcaggtcgtc
     2341 actctcttca ccaccataaa acgcatgcca gttagcgccg tcgcgaatgg ccggtgagcc
     2401 aattcccacc agggcgacat ccagattatc ccagtaggca gagatggttt taaagtgctg
     2461 agactgcatg atcccattac gaattaatgg gttatccagt agagccggaa aatccgcgag
     2521 atgcgattcc cctttcagct tcgctgccgc gctgtaggtt aatgtgttta cgtgatagcg
     2581 gctttcgagt ttaccggacg gtccgccaat aattggtacg cagattaact gccgcgattg
     2641 ccccgcctgc ggcaagtttt caaccagtgc gctcaccgcg cgaccccagg aaaaaccgac
     2701 aatatcgcca ggctccagca agcgatccag cagttgcgcg ccgtgtaacc ccatcatcgc
     2761 cagttgagtc tcttcatcct catcatttcc cgacaccacc acaacgtctt tcaggccaaa
     2821 cttttgcttc agttgctgct ccagccagag attttcgttg tagtcatagt tgatggcgat
     2881 ggtgacaatt ccctgatcgc ggcctcgttt aagcaagcgg ctgatgttgg tgcggtaaat
     2941 acccagttcg cgcgcgattt gcgcctgcgt catatcctgt tcgtaataga gttgggcaat
     3001 cttcacaatc aaacggatat cgtcactgtt ttccattgcc gcctcctggt tctgcacatt
     3061 tgtgcaaata cgctttctca ccgcttgatc tgatataacc ccatcatgat ggcctttcaa
     3121 agcgattttt cactctttgc atggggagaa atagcgcttt ctcttgcaca tttgtttctc
     3181 tgcgcagtag ttatcagcac aggtgtgcag cgattcgtaa tatgttgatt atccttattt
     3241 tgccacgcaa atttgcattg tacttccgga gacacttttg tgataaaagg cgtggcagat
     3301 cacatttaat ttcgacgttg ccgcataacc ctgtttttcc ccgctaagag gtacggtata
     3361 ttccgcgttt tacaggagaa tttatgctgc ccgactcatc agtccgttta aataaataca
     3421 tcagcgaaag cggaatttgc tcacgccgcg aagcggatcg ctatatcgag caaggcaatg
     3481 tgttccttaa tggcaagcga gccaccattg gcgatcaggt gaaacccggc gacgttgtga
     3541 aagtaaacgg tcagttgatt gagcctcggg aagccgaaga tttggtactt atcgccctga
     3601 acaagcccgt aggcattgtc agcaccaccg aagatggcga gcgcgataac attgtcgatt
     3661 tcgttaacca cagcaaacgc gtgttcccga ttggccgcct ggataaagac tcccaggggc
     3721 tgattttcct caccaatcac ggcgatctgg tgaataagat cctccgtgcg ggtaacgatc
     3781 atgagaaaga gtatctggtg acggtcgata aaccgattac cgatgaattt attcgtggta
     3841 tgggggcggg ggtgccgatc ctcgggacgg tgactaaaaa gtgcaaagtc aaaaaagaag
     3901 cgccgtttgt cttccgcatt accctggtgc aggggctgaa ccgtcagatc cggcgcatgt
     3961 gcgagcattt cggctatgaa gtgaaaaagc tggaacgtac gcgcattatg aacgtcagct
     4021 taagcggcat tccgctgggg gagtggcgcg atttaaccga cgatgagtta atcgacctct
     4081 ttaagctcat cgaaaattcc tcttccgagg caaaacctaa agcgaaggcc aaaccgaaaa
     4141 cagcgggcat caaacgtcca gtcgttaaga tggaaaaaac ggcggaaaaa ggcggtcgcc
     4201 cggcgtccaa cggtaagcgt tttacctcgc cggggcgaaa aaagaagggg cgctaatcag
     4261 cgcctgccgc gcgtcgacgt atttgctgac cagctaaatg acgcttcagg atccggttta
     4321 taagcctgct ttttctttaa ctggcgggct tttttcgctt cagcctcacg ctcgaccatt
     4381 aatttatcga tgtactcttt cttaatgctg ttggtttcgg agttggtcag tacgcgacca
     4441 tgcgcaatcc gcgcccgatc cagcagtgtt ttaagttcac gctgctcgcg ttcagtcatc
     4501 tctttttggg taatacgggg gagtgccatg atggtgccct cagtgagcca gagagttcag
     4561 tgtacgggaa ttcgtttgcg agcagaactg ataatattca ttttgccttc tcttaactat
     4621 ttatctcttt cgataattaa ttagagatgc agataaaaaa attaaaggca attattctcc
     4681 gataaagacg ctggttaaca gagtacaggc tcgtttattg atgagcatag tgacaagaaa
     4741 atcaatacgg cccgaaatat agcttccagg ccatacagta tttactcaaa caaattacta
     4801 tgcagttttt gcaccacctg ctcggcatct tcgccgggca ccaggaagca caggttatgg
     4861 ctggatgcgc cgtaacaaat catgcgaatg ttgaacggtt ccagtacgcc gaatacctct
     4921 ttgccaacgc cgcaggcttt tgacaggtca ttgccaatca acgcgaccag cgccagacct
     4981 tcttccacct ccacccggca cagtgcggaa agctccatca gcagagattg cgtcagcaac
     5041 gtatcgccag tggaggttga accggtggta tcaagggtta atgccacgct cacttctgac
     5101 gtggtgatta agtctaccga aatattatgc cgcgcgagga tgccgaaaac ttccgcgagg
     5161 aaaccgcgag aatgcagcat attcaggctg tgcaaagtga gcagagtctg attgcgacga
     5221 agcgccagcg cgcggaacag cggcggattt tcagttttat tgcacaccag cgtaccacct
     5281 gcgcgtgggt ctttgctgga gccgacaaag actgggatat cgctgcgtac tgcgggtagc
     5341 aacgttgccg gatgcagtac ttttgcacca aaagttgcca tctctgccgc ttcggcaaac
     5401 gcgatttcat caatgcgttt tgctgcggaa actacgcgtg gatcggtggt gtagatgccc
     5461 gggacgtcgg tccagatatc aacacgagat gcgtgtaaag cctccgccag caaggctgcc
     5521 gtataatcgc tgcctccacg gccaagcgtc gttgtacgac ctttattttc gctaccgata
     5581 aatccctggg tgatcactaa gccttcattg agacgtggga gcagctgcag cgcggccagt
     5641 tccgccagcg cggctacatc tggctctgca cgaccaaatc ggtcgttggt acgcatcact
     5701 ttacgtacat caaaccactg tgcctgaaca tcgcgttcgc gcaggatctc gacaaacagc
     5761 agggtcgaca tcagctcgcc gtggctgacc agttcatctg tcagcgccgg agacgttgcc
     5821 agcgccgccg cttctgccag aacagtaatg ttctccagca gacgttcaat ctcttcacgg
     5881 ataacgttcg ggtaacgcag acgttccaga atggcaaact ggatgttgcg aatagcgtcg
     5941 agtttttcga atcgctcgcc aggttccagt ccttccgcta aagcgaccag cagattagtg
     6001 ataccagcag aagccgagag gacaactaaa cgcacgttgg catcagaaag cacaatatca
     6061 gcgctgcggt tcatggcgtc aaaatcagct acgctggtac cgccaaattt ggagacaaca
     6121 atttcagaca taactacctc gtgtcagggg atccattttc agccttggca caagggaaga
     6181 gcggaagacg ggtgggcgca gagcgatact tcgctactat tttcacccag aagtgctcca
     6241 ccacttgcga aacgcccgac tgcgaacgct tctggtgaca acccagggga ttcagcccct
     6301 gtagccgatg atgaacgtgg ccagccgttc aatcacctcg gcgatgcacc ccctcaggtg
     6361 ttatcacagg actggctcct ccaacaccgt tacttgggca acgcgcctct tctggcctgc
     6421 gctagcgcag gtagtacatt tataaataaa gggtgagcgg ggcggttgtc aacgacgggg
     6481 tcatgcggat ttttcatcca ctcctggcgg tcagtagttc agctaataaa tgcttcactg
     6541 cgctaagggt ttacactcaa cattacgcta acggcactaa aaccatcaca tttttctgtg
     6601 actggcgcta caatcttcca aagtcacaat tctcaaaatc agaagagtat tgctaatgaa
     6661 aaacatcaat ccaacgcaga ccgctgcctg gcaggcacta cagaaacact tcgatgaaat
     6721 gaaagacgtt acgatcgccg atctttttgc taaagacggc gatcgttttt ctaagttctc
     6781 cgcaaccttc gacgatcaga tgctggtgga ttactccaaa aaccgcatca ctgaagagac
     6841 gctggcgaaa ttacaagatc tggcgaaaga gtgcgatctg gcgggcgcga ttaagtcgat
     6901 gttctctggc gagaagatca accgcactga aaaccgcgcc gtgctgcacg tagcgctgcg
     6961 taatcgtagc aataccccga ttctggttga tggcaaagat gttatgccag aagtcaacgc
     7021 ggtgctggag aagatgaaaa ccttctcgga agcgattatc tccggtgagt ggaagggtta
     7081 taccggcaaa gcaatcactg acgtagtgaa catcgggatc ggcggttctg acctcggccc
     7141 gtacatggtg accgaagcac tgcgtccgta caaaaatcac ctgaacatgc actttgtttc
     7201 taacgtcgat gggactcaca tcgctgaagt gctgaaaaaa gtaaacccgg aaaccacgct
     7261 gttcctggta gcatctaaaa ccttcaccac tcaggaaacc atgaccaacg cccatagcgc
     7321 gcgtgactgg ttcctgaaag cggcaggtga tgaaaaacac gttgcgaaac actttgcggc
     7381 gctctcaacc aatgccaaag ccgttggcga gtttggtatt gatactgcca acatgttcga
     7441 gttctgggac tgggtcggcg gtcgttactc tttgtggtca gcgattggcc tgtcgattgt
     7501 tctctccatc ggctttgata acttcgttga actgctttct ggcgcacacg cgatggacaa
     7561 gcatttctcc accacgcctg ccgagaaaaa cctgcctgta ctgttggcgc tgattggcat
     7621 ctggtacaac aatttctttg gtgcggaaac tgaagcgatt ctgccgtatg accagtatat
     7681 gcaccgtttc gcggcgtact tccagcaggg caatatggag tccaacggta agtatgttga
     7741 ccgtaacggt aacgttgtgg attaccagac tggcccgatt atctggggtg aaccaggcac
     7801 taacggtcag cacgcgttct accagctgat ccaccaggga accaaaatgg taccgtgcga
     7861 tttcatcgct ccggctatca cccataaccc gctctctgat caccaccaga aactgctgtc
     7921 taacttcttc gcccagaccg aagcgctggc gtttggtaaa tcccgcgaag tggttgagca
     7981 ggaatatcgc gatcagggta aagatccggc aacgcttgac tacgtggtgc cgttcaaagt
     8041 gttcgaaggt aaccgcccga ccaactctat cctgttgcgc gaaatcactc cgttcagcct
     8101 gggtgcgttg attgcgctgt atgagcacaa aatctttact cagggcgtga tcctgaacat
     8161 cttcaccttc gaccagtggg gcgtggaact gggtaaacag ctggcgaacc gtattctgcc
     8221 agagctgaaa gatgataaag aaatcagcag ccacgatagc tcgaccaatg gtctgattaa
     8281 ccgctataaa gcgtggcgcg gttaatcatc gttcatgcct gatgccgcta tgtaggccgg
     8341 ataaggcgtt tacgccgcat ccggcaactg gtgcctgatg cgacgcttta cgcgtcttat
     8401 caggcctaca ggtcgatgcc gatatgtaca tcgtattcgg caattaatgc atagcacgat
     8461 taattaaata accttaaaaa caatgccgac tataagtcgg cattgtttta tcagataaat
     8521 ccccttgtct gtgatttaac ggaaatcata ccgtgaggtt aatcctaaaa tagattttta
     8581 atcgtgattg atttcggaaa atactcagat taattacttt tacttttatt ttaaca
//
LOCUS       DAZFTN010000164         8593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFTN010000164 DAZFTN010000000
VERSION     DAZFTN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..260
                     /locus_tag="ACHKV2_004557"
     CDS             <1..260
                     /locus_tag="ACHKV2_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942312.1"
                     /translation="IGYNIINTDLVIGEKIHFRTLSNRSLYFNLTHPPLIATIKDDVF
                     CTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
     gene            complement(1132..1272)
                     /locus_tag="ACHKV2_004558"
     CDS             complement(1132..1272)
                     /locus_tag="ACHKV2_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6942313.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1574..1837)
                     /locus_tag="ACHKV2_004559"
     CDS             complement(1574..1837)
                     /locus_tag="ACHKV2_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6942314.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2262..2387
                     /locus_tag="ACHKV2_004560"
                     /pseudo
     CDS             2262..2387
                     /locus_tag="ACHKV2_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            2452..2712
                     /locus_tag="ACHKV2_004561"
                     /pseudo
     CDS             2452..2712
                     /locus_tag="ACHKV2_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2762..3001
                     /locus_tag="ACHKV2_004562"
                     /pseudo
     CDS             2762..3001
                     /locus_tag="ACHKV2_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3049..3666)
                     /gene="ureG"
                     /locus_tag="ACHKV2_004563"
     CDS             complement(3049..3666)
                     /gene="ureG"
                     /locus_tag="ACHKV2_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO6942315.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3678..4352)
                     /locus_tag="ACHKV2_004564"
     CDS             complement(3678..4352)
                     /locus_tag="ACHKV2_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO6942316.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(4353..4817)
                     /gene="ureE"
                     /locus_tag="ACHKV2_004565"
     CDS             complement(4353..4817)
                     /gene="ureE"
                     /locus_tag="ACHKV2_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO6942317.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4827..6530)
                     /gene="ureC"
                     /locus_tag="ACHKV2_004566"
     CDS             complement(4827..6530)
                     /gene="ureC"
                     /locus_tag="ACHKV2_004566"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO6942318.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(6523..6843)
                     /locus_tag="ACHKV2_004567"
     CDS             complement(6523..6843)
                     /locus_tag="ACHKV2_004567"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO6942319.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6852..7154)
                     /locus_tag="ACHKV2_004568"
     CDS             complement(6852..7154)
                     /locus_tag="ACHKV2_004568"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO6942320.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(7245..7943)
                     /locus_tag="ACHKV2_004569"
     CDS             complement(7245..7943)
                     /locus_tag="ACHKV2_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO6942321.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            8324..>8593
                     /locus_tag="ACHKV2_004570"
     CDS             8324..>8593
                     /locus_tag="ACHKV2_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942322.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDII"
BASE COUNT         2159 a         2163 c         2102 g         2169 t
ORIGIN      
        1 aaataggcta caatataata aacaccgatc tggtcattgg cgaaaaaatt catttcagga
       61 cactaagcaa tcgttcttta tattttaacc tcacccaccc cccattaatc gccacaataa
      121 aagatgatgt gttttgcact cttcatgatt actataacca caataatgaa acgaaatcca
      181 ctattgacaa ttacatatca aaaataaagg ataatcataa taccccatgg ttattaaaca
      241 acgaaaatgt tcaggaataa aaaaaaagag acaattcccg agataaaaca aaagagcata
      301 aacatatgaa ccggaaattc tatttcaaaa atatcctgtg gggaatgtgc cccattataa
      361 tatccatatt gcatctgata tatgacacac caacagaagc atcgataaaa gttgacttat
      421 ttctgatact cagtggcctg gcccttcctt gttcaatata cctgacaaaa acaatcacac
      481 taaaatacac atccatatca ttctggaaaa aacctctttt ctggtcttat ggtatatatc
      541 attttcatct tgtttatata atgatatgta tagttttagc aataccttct gcaattatcg
      601 ttttattctt tcacttttat caaaacatca ccagaaactg gaagtgacaa ccgcaataat
      661 gaacataaaa aggcaaatca gaatggcagt aaatcaataa agtaccattt catatgaatc
      721 atcagaaaaa cggtggacgc gacatccagc acgaggcaaa acacagcaac aaaataagcc
      781 ggatatcgcg ccctgtactt ttctaactca actcccggca ttgtgcaagt tttaatcgca
      841 ggtggattca gagccacgcc cccattgatt cagcctgaag acacgtatat ccttggagaa
      901 agcaggaatc tcattgctgt gaaattcctt acagaatctt cagccccatt ttatggggct
      961 ttttttggtg cccccacgct gttaccacac tttcgggatg cgaataaaaa agcccacagt
     1021 ttttacactg cggacttttg ttttttcttt gtgatttgtg tgcaagggtg cgactttcga
     1081 ccgaaccttt ggcaaccgga tagcggggac tgttgtcccc gctgcggttt cttacggttt
     1141 acactgcaag aacgccgcaa tttccgacca catgaagctt accctgaatt cacacggtga
     1201 tgcgtgtaac acccagacgg tcagcaacat ggtcaggcat aacatgccca caatgatcgt
     1261 tttttgcggc atagcacttg ctccttttac agagaggcgc taaccttcca cttgctgaag
     1321 gtagatggtc agggcctcgg gttaacgttt aagttgactc ggggcctttc cacatccggc
     1381 cttcaggtaa tccttccagc cgtcagccga aaggcacccg cgcgtaatgt actggttttc
     1441 agagaggtcc gcaatcttcc ggtgacgaga tcatctgcac acgccccggt gggacgggct
     1501 aacggtatat tcaggtgcag gatctctctg tctggtgcgt ttttaaattg tcaaacgcac
     1561 cctggcggca aaatcagcca ccttttttcg catcaacaaa acgcccaaaa cgctgggtaa
     1621 agcgtgcaac attaccttct gaagcgacag ttctcagttt acctgtataa aacggatgcg
     1681 acttagaaga aacgtcaatt gtaacatatg ggtaagttac accatccagt tcaattaccc
     1741 ggtcagtttt gatcgtcgaa ccgatcctga agtactcatc aatactggta tcgtgaaata
     1801 ctactgtacg gtattccgga tggatattcg gtttcacaac agttttcccc ctttaagtga
     1861 aaattccgca taaaggtaca tattcagacg gtcagattca actgtctgtt cttataacct
     1921 cactctcttt cagtattaca atatgttata taattataaa ctggacttcc cgaaaaaatc
     1981 cattaaataa gaagcgagcc gttgcggagg cttgctctct cctttaacct catgtgcaat
     2041 atccgtaacg cgctcacata aatcatcatc tgataatata acaacaccca ttattttatt
     2101 ctgacctaaa aacttcttat gaaataaacg tttacccgac gtatctgatc ccgctgtttt
     2161 tacctgtatc ctttaacata cttgagaagc ccgctgaaaa aatataccgt aaaggcggtt
     2221 tctctgtctt ccataaacct gtcggataag aagggcatta aatgaaaacc aaaaaagtta
     2281 tgatgattgc actggtcagc tctgcgctgg ctctttcagg atgtggagcc atgggcacag
     2341 cgatcaaaaa acgaaatctg gaagtaaaaa cccagatgag tgaaacctga ggtagcctga
     2401 gtttaacgga cactccttcc tgaaatagaa tggcatcaga aggagctaat aatgagcaga
     2461 aaaacccaac gttactctaa agagttcaaa gccgaagctg tcagaacggt tcttgaaaat
     2521 caactttcga tcagtgaagg cgcttcccga ttatctcttc ctgaaggcac tttaggacaa
     2581 tgggttaccg ccgccagaaa agggctcggt actcctggtt cccgcacggt ggctgaactg
     2641 gaatctgaaa ttctgcaact gcgtaaggcg ttaaatgaag ctcgccttga gcgagatata
     2701 ttaaaaaaag cagctgtaga ttcaatctgt caatgcaaca cccctttcaa ttatctcttt
     2761 cactgcccgc gacggtatcg gttatcagtt tgacctgctg gctaacggtg aaaccgtact
     2821 gcgtgatgca tccggcgaaa aacgcatcaa gggtgaaaaa gacggtcgta tgctcatgaa
     2881 tgttggcctg aatgcggaaa ttcgcgacaa catccgcttc ggtctggagt ttgaaaaatc
     2941 ggcatctggt aagtacaacg tggataacgc ggtgaacgcc aacttccgtt attccttctg
     3001 ataaaaacaa ggggccgcct gccggccctg atacagagga tgttgtgatt acttacccag
     3061 catgccctgc tcttcgataa aggcgatgat gttttgcaga ccttcaccac ttttcaggtt
     3121 agtgaacccc catgggcgtt ctccgcgcat acgtaaagta tcacgctcca ttacctcgag
     3181 ggaggcgccc acgtatggtg caaggtcagt tttattgatc acgaggaagt cggatttggt
     3241 gatccccgga ccgcctttac gcgggatctt ttctccttct gccacatcta tgacatatat
     3301 ggtaaggtcc gccagttcgg ggctgaaggt cgcactcagg ttgtcgccgc cgctttccac
     3361 aaagatcagc tcgagattac caaatttctc actcagtgct tccacggctg ccagattcat
     3421 tgatgcgtct tcacgaatgg ctgtatgagg gcagcccccc gtttcgacac cgacaatgcg
     3481 ttccgcttcc agtgctccgg cctcagtgag aatgcgttga tcttctctgg tgtagatgtc
     3541 gttggtgact acggctaatt gccaggtgtc acgcatggat ttgcagaggg cttccagaag
     3601 cgcggtttta cctgagccga ccggaccgcc gacgccgaca cgcagcggtt gcttgattat
     3661 gttcatttta ttttctccta agaacggaat aatcgggaat actgggtttc atgtcgcgcg
     3721 gaagcgatag atgcaagtgg cgttgctgaa ccaattgcgt gatccggggt tgcaagggca
     3781 ctgtccatgt tcgccgcata gcgggcacac aggcgcataa ttaactgctg ggcagccagc
     3841 tggccgtagg gtaccagcct gacgcccgcc atcactgcgc tttcaatcca gctgtagcca
     3901 aggctcaggg ccaggtctga aacggatatt tgccagcgta cgccgagcca tgccataccg
     3961 gttaactggc tttgctggca tagtttgcgc cattcggccg ggcagtcggg ctgccagtcg
     4021 accagaagtc tggtgaaggc tgcaccgcgg ttacgttctt catcgcgtag ctcacgggtt
     4081 tccctgcagg ccagcagata agcggtccag cgcccggcac tgtcaggatc gcccgcttca
     4141 caggcccggt atagccgggc aagcagtggc aggtcgacgg caaaaaaact ctgttccatc
     4201 tgcaactgct gccagtgttc aaaggcggcg ctatcttcta cccagcctgc ttccactgcc
     4261 cactccagtc cctgggacca gctgtagcct ccaacaggga ggctgttact ggcaagctgc
     4321 atcagccgca gacgtttttc cggagtcggc attcagtggc tgtgctcctg atcgtgatta
     4381 tgggcgtgcg acttgctggc gtaagcgccg gcttccggct caaacggaag atgtgcaaaa
     4441 tcaacatcca gcccaaactg gcgcagcata tcatcgagga cgtgatcgtg atggtagcgc
     4501 agctctccgg gcattatttg cagcggaacg tggcgattgc cgagatgcca gcaggctttc
     4561 gcgagcataa aaggatctgc gcagcgcact acagaaacgg cttcatcagc ggcgatcacc
     4621 ttaatgaatt catcaccgtt ttcgttactg agaatatcac catcgcgcag cagcagaccg
     4681 cgtggcagca acagtccagc ctgacgtcca tcgttaaggg tgactttaat acggcttttt
     4741 acccgcatgt caacagggag ggtaacgctg gcagttgtct gagccggggt ctccacccgc
     4801 cgggtcaaat aaagcatttg tgctccttaa aacaggaaat aacgttgcgc cattggcaga
     4861 atgtccgccg gttcactggt tatcagttcg ccgttgatac gcacttcgta ggtttgtgaa
     4921 tccacggtta tatcaggcaa caggctgttg tggcgcatat cggctttttg tactgtgcgg
     4981 cagcctttta ccacagcagt tgtgctgtgc aggttaagct gttcagcgac gccatttgct
     5041 gctgctgcct gcgacaggaa agtcacacga cagcggtgac gggcactgcc caatgcagcg
     5101 aacattgggc gatagtgcac cggctgcggt gtggggatag agccgttgat atcacccatc
     5161 ggcgccatgg ctatcattcc gcctttgacg atagtcgccg gttttacgcc aaagaacgcc
     5221 ggggaccaga gcaccagatc cgccagtttt cccacttcaa tcgagccgac ttcatgagca
     5281 ataccgtggg ttaatgccgg attaatggtg tatttagcga tatagcgctt cacgcggacg
     5341 ttgtcgttat caccactttc ttccggtaac gggccgcgct gaactttcat ccggtgtgcc
     5401 acctgccagg ttcgtaacac tacttctccg acgcgtccca tggcctgcga atcggacgag
     5461 gtgagggaga acgcgccaag gtcgtgcagg acgtcttccg cggcaatggt ttcctggcga
     5521 atgcgcgatt cggcaaaggc tacgtcctcg gcgatatccg ggtcaagatg atggcaaacc
     5581 atcagcatgt ccagatgctc atcaatagtg ttgacggtat agggtagcgt cggattggtt
     5641 gaggaaggca gaatattggg gtgcgcgcag gcggtgataa tatccggagc atgaccacca
     5701 cctgcacctt ctgtatggaa ggtatggata gtgcgcccgc cgatggcagt cagagtatcc
     5761 tcaacgaatc ctgattcgtt aagcgtatca ctgtgtagcg caacctgaac gtccatttcg
     5821 tcggctacag tcagtgcgca gttgattacc gcaggtgtag ctccccagtc ttcgtgaatt
     5881 ttgaggccga taaccccggc cgcgacctgc tcacgaagcg catccggatt ggagcaattg
     5941 cctttaccca gcaacccgat attgaccggc aggctgtctg cagcctgcag catttgataa
     6001 atgtaccatg gtcctggggt acaggttgtg gcgttagaac ccgctgtcgg gccagtaccg
     6061 ccaccgatca tggtggtaat gccggatgtc agcgcttctt cagcctgctg tgggcagatc
     6121 cagtgaatat gcgtatcgac gccacctgcg gtaacgatcc tgccttctgc ggcaataatt
     6181 tccgtggata cgccgattgg gatagtgacg ttgggttgta tatcaggatt accggctttg
     6241 ccgatagcaa atatccttcc atctttgacg ccgatatcgg ctttaacgat cccccagtaa
     6301 tcgatgatca gggcattggt cagcaccaga tcagcgcagc cggcggagag catttgcccc
     6361 tgtcccatac cgtcgcggat tactttaccg ccgccgaatt tgacctcttc gccgtaggta
     6421 gttaaatcat cttcgacctc gatccacagc tcagtgtctg ccagacgaat tttatcaccg
     6481 gtggtagggc cgaacatgtc agcataggcc tggcgtgaaa tattactcat catttgcctc
     6541 cagctcaccc attatatctc cgcggaaacc gtgcacgata cgggcaccgc tcagcgccac
     6601 cagttcaacc tcacgctttt ggcctggttc gaagcgcact gcagttccgg cggcgatatt
     6661 gagacgatag cctcttgcct tctggcgatc gaacttcagt gccgggttaa cctccgcgaa
     6721 gtgataatgg gaaccgacct gaattggtcg gtctccgtga ttttcaacga taatactgca
     6781 ggtagcccgg ccgatgttaa gctctatata gccaggttta accttatatt ctccagggat
     6841 catctgttac ctcagattat cggattatgg acggtaacca gcttggagcc gtccggaaag
     6901 gtggcttcga cctggatatc ggggatcatt tctggtatgc cttccatgac ctgctcgcga
     6961 ctcaggacat gccgtccttc ttccatcagc gcagcgacgc ttttgccgtc gcgagcgccc
     7021 tccattataa aagcgctaat cagggccacg gattcgggat agttaagttt caggccgcgg
     7081 gccagacgac gctctgccag cagtgcagcg gtaaacagta atagtttgtc tttttctctt
     7141 ggagtcagtt ccatagcgtt ctcttaagtc agccagatcc ggggaagtac cggtgatttg
     7201 tcagtcagtt gcggccgcat aaattgccag atatcacgca ttacctactg gcaacgcaga
     7261 ttatcatcac ttaaaaagcg aactgtcagt agaccatcgg tgagcgtggc tccggcgtag
     7321 ttttccagtg gtgtgagttt ttcgcgtatg tcttccagtt gcgtttcatt ccccaggtag
     7381 aacagcatcg ttcccaccca tggtttccgg gcaacgcagg taagttctcc attggccacg
     7441 tgcagacgct caatcaacag cggagaaccg tcgacccata tctccagacg gttagcgagt
     7501 gttccgtggc tgaaggtttc tccgatgacc ggacgtccaa ggcacagaag atcccaggcc
     7561 agcagtgtac tggaggacga cagatgaaac acggtatgca acgtggcgtg agcgccggga
     7621 aaaaaaatcg aatcctgtgg caaccactcc agcgttgcgt tattgcacag cgtcaggttt
     7681 tgctgaaggt gcgcctgcgc gccgctgctg cggtaaaatt tactggcacc aggcatggtg
     7741 atcagcgtat ggcaatcaga gtcaattatt gcgttgatga tgagttcgtc accgccgacg
     7801 ataccaccgg gtggatgaag cagacagagg tggcaggttt cttcttcggg ataaaaagga
     7861 cgctgaacag tgagcagacc gacatgatga cgggtggtga gcgtggtttt cccgtgcaga
     7921 cgctgaaaac ggagatccag catggcctgc cagccttttt tattttgtgg taacgcggta
     7981 ctgagcaatc ttacgcccct gataaataaa gataaaacga cagtaaagag ccggatttcc
     8041 ttgttatata aaagatgatg cctttccagt gagttttcgt ctgaataata cgaatgtttg
     8101 ttttcgtcat gaagcgttcc ggctcccggg gctatacatc gccagaaaag caatgcatat
     8161 tgtcgggatt atccagagaa tcaggcagaa agatccgaca atgggctgtg attatgccat
     8221 ctggctcaga atgatatcaa ggtgttatgg ggataacttg ctgatacaat agcagtatga
     8281 cagattcatt aatgcggaaa ctgctcactg aaacagagtg ttcatgactg agcactggtc
     8341 gggtcatgca gatgataatt tccccttttc ttggggaagc gacagtattt tcatgcatgc
     8401 ctgtcttcag tcatacgggg agagcatctg tgatacagtc catacatttg ttgcggacgt
     8461 cgctcctgag tcactgactg gtctgcaaac agtttatctg tacacggcag ggcttctgtg
     8521 tgatatcgtt tttcatatcg agggggggcc tccagagatg aatggaataa taatatttcc
     8581 tgatgatata ata
//
LOCUS       DAZFTN010000165         8473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFTN010000165 DAZFTN010000000
VERSION     DAZFTN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1743
                     /gene="espL4"
                     /locus_tag="ACHKV2_004571"
     CDS             <1..1743
                     /gene="espL4"
                     /locus_tag="ACHKV2_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO6942323.1"
                     /translation="PQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKK
                     CKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVDNHALTVRL
                     RIKNTTEGCIHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDH
                     VINQSVAIIPALPKEQLLMLKGTVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKM
                     PELHRTEMLTAYNSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILE
                     AKDKNGFSGLFLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVL
                     YHVMANGDADMLKIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSD
                     IVKVILEALPSLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALP
                     TLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTA
                     EDIMDFWQHKAPQKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNY
                     MEALLKKASPHTVR"
     gene            complement(1712..3448)
                     /gene="aceK"
                     /locus_tag="ACHKV2_004572"
     CDS             complement(1712..3448)
                     /gene="aceK"
                     /locus_tag="ACHKV2_004572"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO6942324.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3632..4936)
                     /gene="aceA"
                     /locus_tag="ACHKV2_004573"
     CDS             complement(3632..4936)
                     /gene="aceA"
                     /locus_tag="ACHKV2_004573"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO6942325.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4966..6567)
                     /gene="aceB"
                     /locus_tag="ACHKV2_004574"
     CDS             complement(4966..6567)
                     /gene="aceB"
                     /locus_tag="ACHKV2_004574"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO6942326.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6836..7765)
                     /gene="metA"
                     /locus_tag="ACHKV2_004575"
     CDS             complement(6836..7765)
                     /gene="metA"
                     /locus_tag="ACHKV2_004575"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO6942327.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7922..8365
                     /locus_tag="ACHKV2_004576"
     CDS             7922..8365
                     /locus_tag="ACHKV2_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6942328.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2157 a         2079 c         2086 g         2151 t
ORIGIN      
        1 ccccaaaacc gtatagtatg ccgacacatt gctgcacaat atattaatga tatttatcag
       61 aatgttgact acaaacccca tcaagatgat tactcttcag ctgaaaaatt tctcactcac
      121 ttcaacaaaa aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc tgaggggcgt
      181 tgcgttgctg cctgcggtga tttcgggcta gttatgaagg catattttga caagatggaa
      241 tcaaatgacc tcagtgttat ggctgccata ttattggtag ataaccatgc tttgacggtc
      301 cggctaagaa taaagaacac aactgaagga tgtatccatt acgtggtttc ggtttatgat
      361 cctaatgtaa ctaacgataa aataagaatc atgagcgaaa gtaaagagga tattaaacac
      421 tattcactga tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat
      481 gttattaacc aatctgttgc aataattcca gcacttccaa aagaacagct attgatgcta
      541 aaaggaactg tggatgaaat aacccctcca ttatcacctg caacgatgaa tttgctaatg
      601 gcaattggtc agaatcacca acttaagcaa ctgatgattc aactccagaa aatgccagaa
      661 ctacatagaa cagaaatgtt gactgcctat aatagtatta acctccccgg tttatatttg
      721 gctataaatt atggtaatgc ggatatcgtt gagactattt ttaattcatt gtcagagcca
      781 ggatatgaag gattactctc gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa
      841 aatggttttt ctggattatt tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg
      901 attctgaacg ccttacctaa actggccgca acacatcatt tagataacga acaagtgtat
      961 aaattcctga gtgccaaaaa tagtacgtcc agccatgttt tataccatgt tatggcgaat
     1021 ggtgatgccg acatgctgaa aattgttttg gacgcgttat ctttgttaat tcgcacatgt
     1081 catttgacta aagaacaggt actcgatctc ctgaaggcaa aggattttta tggttgccca
     1141 ggactatacc tggcgatgca aaatggacat agcgatatcg tgaaagttat tcttgaagca
     1201 ttgcccagcc tggcccagga aattaacatt tcagcttccg atattgtaga tcttctgacc
     1261 gctaaaagtc ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg acatatgaac
     1321 gttattaata ctatttttaa cgcattaccc actctgttta atacgtttaa attcgataaa
     1381 aaaaatatga agcccctcct cctggcaaat aattctaatg aatacccagg tttgttttca
     1441 gcgatacagc ataaacagca aaacgttgta gagatggttt atcttgcttt atctgaccat
     1501 gcacgcctgt ttggatttac cgctgaagat attatggatt tttggcaaca caaagcgcca
     1561 caaaaatact ctgcctttga gttggcttgt gaattgggtc accgggttat tgctgaatta
     1621 atctttaata cattaaataa gatggctgaa agctttggct ttacggataa ccctcgatac
     1681 attgcggaga aaaattatat ggaagcttta ctcaaaaaag catctcccca taccgtacgc
     1741 taaatctttg cctgcgccga tacgcataaa catcttccac atgcccttca cgtatgcggt
     1801 tttgtagtgc gcgccagtaa tcagcgcgga acaggtcggc gtgcatctct tcaaacagcg
     1861 gaccaatacg cgggtcggcg catagccaat ggcgaaactc ttccgggaaa acatcgcccg
     1921 gcgagacgct gtaccacggt tcgctggcaa gttcgtcttc cgggtagcgc ggcggcggga
     1981 tatcgcggaa attcacttcc gtcatgtagc aaatttcatc gtaatcataa aaaaccacac
     2041 gcccgtgacg ggtgacaccg aagtttttaa acagcatgtc gcccgggaaa atgttagcag
     2101 cggcaagctg gcgaatggca ttgccgtact cttcaatggc gtcgcgcagc tgctgacctt
     2161 ccacttgttc cagccagata ttgagcggca ccatccgccg ctcaatataa agatggcgaa
     2221 tcacaatttg ttcgccgaga tcggtgattt tttccgctgc ttcctggagc agtaattcca
     2281 ttaatgccgg ggaaatatgc cgcttctcca gcacaaagtt ttcaaactcc tgggtatccg
     2341 ccattcggcc cacgcgatcg tgctctttca ccagttgata gcaggcacga acgtgagcgg
     2401 cagacatctc tttctgcggc gcgaacttgt ctttgatgac tttaaataca cggtcaaagc
     2461 ccggcagcgt gaacaccaac atcaccatgc cacgaatacc cggcgcttca atgaactgct
     2521 cattacagcc ctgtagataa acgagatatt cgcggtagct ttcggttttg gcgtgcttct
     2581 ggcagccgat agccatatac aattcagcgg tggttttacc tggcagaatt tcccgtaacc
     2641 actcgaccag cgctgcgggc agcggcgcat aaaccataaa ataagaacgc gcaaagccaa
     2701 aaacaatgct cgcttcggcg gtcgtcgtca gacaggtatc aataaacagt tcgccgtcat
     2761 ccgtctggtg gatcggcaat aaaaatggca atgtgccgga aggcgttatt agtttgccca
     2821 ccagccaggc ggctttatta cggtaaaaca gttcgttcgc cacctgtaaa tgacttttgg
     2881 agaggttttc tggccccaac gtttccgtca gatggcgaat aatgtaatgg atgtcacggc
     2941 ttttattctg ccagtgcaga cgcaacggca ggtcgctgat aacgcgcatc agcagagatt
     3001 cccagccgtg atcggggtga aagtctttcg ccagcgggcg gggaatggta cgaaagcggc
     3061 gctctggctg ggagctaaag ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata
     3121 accgacagta cacggagtta aaaaagctct ccgcaatctc gaagcgcggg taatccggca
     3181 acagccgggt gtaatgctct ttgacgcgca gtaaaaattc cgcgtccgtg ctttggccgt
     3241 tggtaatgca gcgcagttgc tccacgacca gaccaacgtg atgatcgtaa agatggatac
     3301 ggtttttcat cgcctgctgg acagcatgcc agtcggcttg ttcgaaacgc tgctgcgccc
     3361 cggaagtcac ttcgaggaat cgaccatact gagcgtcgaa gccttgcaaa atggtttgag
     3421 caatcagtaa ttccaggcca cgcggcattt agcgccctca tcaggagcag agaattgccg
     3481 gatgcggcgt aaacgcctta tccagcctac gttcggcaac ggttgtaggc atgataagac
     3541 gcgcaagcgt cgcatcaggc atcgtgcacc gattgccgga tgcggcgtat atgccttatc
     3601 cggcctacag tcagcaacgt ttgtaggttg cttagaactg cgattcttcg gtggagccag
     3661 tcagcgcggt aaccgacgac gtgccgccct gaataatggt cgtcacttta tcgaagtaac
     3721 ctgtacccac ttcctgctgg tgagatacga aggtgtagcc atctttcgcg gcggcgaatt
     3781 ccggctgctg cactttctca acatagtgct tcataccctc gccctgggca taggcgtttg
     3841 ccagatcaaa catgttgaac cacatgctgt ggatacctgc cagggtgatg aactggaact
     3901 tgtagcccat atccgacagc tgctgctgga agctggcaat agtgttgtcg tcgaggtttt
     3961 tctgccagtt gaacgacggc gagcagttat aagccagcag tttgcccgga tatttcgcgt
     4021 ggatagcttc cgcaaagcga cgcgccagtt ccagatccgg cgtggaggtt tcacaccata
     4081 ccaggtcagc atatggcgca tacgccaggc cacggctgat cgcttgctca atgcccgcat
     4141 gagtacggaa gaagccttca ctggtacgct cgccggtaat aaattcgctg tcatacgggt
     4201 cgcaatcgga ggtgatcaga tccgccgcat cagcatcggt acgtgcaacc agcagggttg
     4261 gaacgcccgt cacgtcagct gccagacgcg ccgcgaccag tttctgaata gcttcctgag
     4321 ttggcaccaa aactttgccg cccatgtgac cgcatttctt cactgacgcc agctgatctt
     4381 cgaagtgaac tgccgctgca ccggcttcaa tcatcgcttt catcagttca aaggcattca
     4441 ggacaccgcc aaaaccggct tccgcgtcgg caacgatcgg caggaagtaa tcgacatagc
     4501 gcggatcgcc cggctcaatg cccgcggacc attggatctg atccgcacgg cggaaagtgt
     4561 tgttgatccg ctccaccaca gctggcaccg agtttgccgg atagagcgac tgatccggat
     4621 acatgctggc cgccaggtta gcgtccgccg ctacctgcca tcccgacaga tagaccgctt
     4681 caatacccgc tttcgcctgt tgcagcgcct gaccgccagt cagtgcaccg aggctgttga
     4741 tgtagccttt tttcgactcg ccatgcagca gacgccacat tttcgccgcg cccagttgcg
     4801 ccagcgtgca ttcaggattg actgaaccgc gtaatttcac cacatcttcc gcgctgtatg
     4861 ggcgagtaat tccttcccaa cgcggttgag tccactcttt ctgtaattct tcaatttgtt
     4921 gtgtacgggt tttcatgtgc agatgctcca tagttatgtg gtggtttacg ctaacaggcg
     4981 gtagcctggc agggtcagga aatcaattaa ctcatcggaa gtggtgatct gttccatcaa
     5041 gcgtgcggca tcgtcaaaac gcccctggga gaaacgttct tcgcccagtt cgctggcaat
     5101 gactttcatc tcttcgccca gcatctggcg gaacaaggct ttagttaccg gtttgccatt
     5161 gctcaacgtt ttttgatgat ggatccactg ccagattgag gtacgggaaa tttcagccgt
     5221 cgccgcatct tccatcaggc cataaatcgg cacgcagccg ttgccggaga tccatgcttc
     5281 gatgtactgc acagccacgc gaatgttggc gcgcatacct tcttcggtgc gttcaccatc
     5341 acaaggtgcc agcagctgat cggcagtaat cggcgcgtct tgttcgcgca tcacttcaag
     5401 ctgattttta cgggagccga gaatgtcgtt gaataccgcc attgccgtgt ccgcaaggcc
     5461 tgggtgagcg atccatgtgc catcgtgacc gttattggct tccagcgatt tatccgcttt
     5521 tactttgttg agcacctggt tattgtgctc ttcatctttg ctcggaataa acgccgccat
     5581 gccgcccatc gcaaaagcac cgcgtttatg gcaggtttta atcaacaggc gtgagtaagc
     5641 attcaggaat ggtttatcca tcgttactgc ctgtctgtct ggcaggacgc gatcgggata
     5701 gtttttcaac gttttgatat agctgaagat gtaatcccaa cgaccgcagt tcagaccaac
     5761 aatatggtca cgcagcgcgt gaaggatttc atccatctgg aacacggcgg gcagcgtttc
     5821 aatcagcaac gtcgccttga tggtgccgcg cggcagatta aagcgatctt ctgcatagct
     5881 gaagacttcg ctccaccagg ccgcttcctg ccaggactgg gttttcggca gatagaaata
     5941 gggaccactg ccctttgcca acagtgcctg atagttgtgg aagaaataga gcgcaaaatc
     6001 aaacaggctg ccggggattg cctcaccacg ccaggtgaca tgtttttccg gcaagtgcag
     6061 accgcgtacc cgacaaatca aaaccgctgg attgggcttg agctggtaaa ttttgcctgc
     6121 ttcattggtg taactgatgg tgccgttaac cgcatcacgc aggttaattt gcccgtcgat
     6181 cactttgttc cagtctggtg ccagtgaatc ttcgaaatcg gccataaaga ctttcacatt
     6241 ggcgttgagc gcgttgatca ccatcttgcg ctctacggat ccggtaatct ctacgcggcg
     6301 atcttctaag tccgcaggaa tcccgcgaat tttccagtca gcatcacgaa tggaagctgt
     6361 ttccgaaata aaatcaggca acgttccgtt atcaatatct tgctgctgct gaatgcgcgc
     6421 tgccagaagt ttattgcgtt gtggcgtaaa atgcgtcacc agctcagtca gaaattctac
     6481 cgcttcggca gtaagaattt gcttctcctg ctcgccatac ggccttatga aagccagttc
     6541 atcggttgtt gttgcctgtt cagtcatcgt gcagctcctc gtcatggatc cgaaaacttc
     6601 cccactgtga acgaaggatc gttgtgcgct tttcgttcag cacaactaag actactcatt
     6661 taaaaatcaa aatcaaaaac aatttccatt ttaattaaaa atacttgata acttattgat
     6721 aacaatatta ataaaattta ttcacctact gaggtgtgtt tcggaaataa aaaaggcacc
     6781 cgaaggtgcc tgaggtaagg tgctgaatcg cttaacgatc gactatcaca gaagattaat
     6841 ccagcgttgg attcatgtgc cgtagatcgt atggcgtgat ctggtagacg taatagttga
     6901 gccagttggt aaacagcaaa ttaccgtgac tacgccagct cgctcgcggt ttgttttgcg
     6961 gatcattgtg cgggaaatag ttatacggta cttccgggtc tagtccggct tccacatcgc
     7021 ggaaatattc ctgcgccagc gtttgcgcat catattcggg atggcccgtc acaaaggcaa
     7081 tgcgcttatc tttactggca aacagatatg catccccttc ttccgtctct gccagaattt
     7141 ccagatcggt gtaatcacga atcaacgctg ccggaaagtc agcatagcgc gaatgcggtg
     7201 ccaagaatga atcatcaaag ccacgcgtca gaagcgcatg aggatggaga atatgatgct
     7261 cgtaaacgcc agagagtttg tcggtgcgag tttgcttagg aatgccgtag aggatattga
     7321 gcgctgcctg taccgcccag cagacaaaca gcgtcgaggt gacgtgatct ttcgaccact
     7381 ccagcacctg tttgatctgc ggccagtaag cgacatcatt aaactccacc aggcccagcg
     7441 gcgcaccggt tacaatcaga ccgtcaaagt tctgttcctg aatatcttca aagttacagt
     7501 agaagttgtt cagatgctct gcgggcgtgt tacgcgattc acgggaatcg atgcgcaaca
     7561 gctgaatatc gacctgcaaa ggtgagtttg aaagcaggcg cagaaactga ttttcagttt
     7621 caatcttctt cggcatcagg ttaaggatca gaaccttaag tggacgaatt tcctgaccag
     7681 acgcacgaga agttgtcatc acaaagacgt tttcttcacg caagaaattg acggcgggta
     7741 gctcgtccgg cacacgaatc ggcataacct gattacctca ctacatacgc ttatacgttt
     7801 agacatccag atagctgaag ataaccagaa aatttgccaa tgtcgagcct gcatgttgaa
     7861 ggtgagaaag tttcaaggaa aagactacgt tagaatataa gaatgactaa aaggagagaa
     7921 aatggttatt agtattcgcc gctcacggca tgaggaaggg gaggaactcg ttgcgatttg
     7981 gtgtcgatct gtcgatgcca cccacgattt tttatcagca gagtatcggg ccgagctgga
     8041 agagctggtt cgttcctttc tgccggaagc accgttgtgg gtcgcggtta atgaacggga
     8101 tcagccggtt gggtttatgt tgttaagtgg gcagcatatg gatgcgctgt ttatcgatcc
     8161 tgatgtgcgc ggctgcggcg tgggccggat gctggtgaag catgcgctct caatggcccc
     8221 ggaactgaca accaatgtta atgagcaaaa tgagcaggcg gttgggttct ataagaaggt
     8281 gggttttaag gttacgggac gctctgaggt ggacgatttg gggaaaccgt atccgttgct
     8341 ggatctggcg tatgtggggg aataagctgc cccctatcgt gcctgcgtaa gctggataaa
     8401 acgcgccagc gtcgcatccg gcattttttt gcaccaacgc aaaaaggcca tccgtcagga
     8461 tggcctttcg ctt
//
LOCUS       DAZFTN010000166         8471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFTN010000166 DAZFTN010000000
VERSION     DAZFTN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..331
                     /gene="dinJ"
                     /locus_tag="ACHKV2_004577"
     CDS             71..331
                     /gene="dinJ"
                     /locus_tag="ACHKV2_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO6942329.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            334..610
                     /gene="yafQ"
                     /locus_tag="ACHKV2_004578"
                     /pseudo
     CDS             334..610
                     /gene="yafQ"
                     /locus_tag="ACHKV2_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            766..1506
                     /gene="dpaA"
                     /locus_tag="ACHKV2_004579"
     CDS             766..1506
                     /gene="dpaA"
                     /locus_tag="ACHKV2_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO6942330.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1477..2244)
                     /locus_tag="ACHKV2_004580"
     CDS             complement(1477..2244)
                     /locus_tag="ACHKV2_004580"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO6942331.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2349..2927)
                     /gene="lpcA"
                     /locus_tag="ACHKV2_004581"
     CDS             complement(2349..2927)
                     /gene="lpcA"
                     /locus_tag="ACHKV2_004581"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO6942332.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3167..5611
                     /gene="fadE"
                     /locus_tag="ACHKV2_004582"
     CDS             3167..5611
                     /gene="fadE"
                     /locus_tag="ACHKV2_004582"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO6942333.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5654..6100)
                     /gene="ivy"
                     /locus_tag="ACHKV2_004583"
     CDS             complement(5654..6100)
                     /gene="ivy"
                     /locus_tag="ACHKV2_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO6942334.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6281..7051
                     /gene="yafV"
                     /locus_tag="ACHKV2_004584"
     CDS             6281..7051
                     /gene="yafV"
                     /locus_tag="ACHKV2_004584"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO6942335.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(7169..8341)
                     /locus_tag="ACHKV2_004585"
     CDS             complement(7169..8341)
                     /locus_tag="ACHKV2_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFO6942336.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLNLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         2061 a         2118 c         2084 g         2208 t
ORIGIN      
        1 tagattgtcg ctgttgctca tttgagctac aattcaagct gaataaatat acagcatagg
       61 agatatccca atggctgcta acgcatttgt tcgcgcccga atcgatgaag atctgaagaa
      121 tcaggcggcg gacgtactgg ccgggatggg gctgaccatc tctgacctgg ttcgcataac
      181 cctcacaaag gtcgcgcgtg aaaaggcatt gccgtttgat ttacgcgagc ctaatcaatt
      241 aaccattcaa tcaatcaaaa acagcgaagc tggcgttgat gttcataagg ccaaagacgc
      301 cgatgattta tttgataaat taggagttta aatatgattc aaagggatat tgaactcggg
      361 acaattttca aaggatgtaa aacttgcaca aaagcgtcat aaggatatga ataaattgaa
      421 atatcttatg acgcttctta tcaataatac tttaccgctt ccagctgttt ataaagacca
      481 cccgctgcaa ggttcatgga aaggttatcg cgatgctcat gtcgaaccgg actggatcct
      541 gatttacaaa cttaccgata aacttttacg atttgagaga actggaactc acgcggcgct
      601 ctttgggtaa agcccgctgc cgatgaaatt tgtaaaccct gcgccgacag gcttgcgtaa
      661 aactcttttg gtgataatgt gagccttccc aatccagact attccgattt gggatccatt
      721 ttgccgtcct ggcgtgtgta accgttttat caaggaataa gcagtatgcg taaaatcgca
      781 ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg ccggtttgct gggcagcagt
      841 agctcgacca cgccagtgag caaagagtat aagcagcagt tgatgggatc ccctgtctac
      901 atccagatct tcaaggaaga acgtacgctc gatctctacg tcaaaatggg cgagcaatat
      961 caactgctcg acagctataa aatctgtaaa tattccggcg gcttagggcc aaaacagcgt
     1021 cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca gttaaaacca
     1081 gacagccgtt actacaaagc gattaatatt ggtttcccca atgcctatga ccgtgcgcat
     1141 ggttatgaag ggaaatacct gatgatccac ggcgattgtg tttccatcgg ctgctacgcg
     1201 atgaccaatc agggcattga cgagattttc cagttcgtta ctggcgcact ggtatttggt
     1261 cagccgagcg tgcaagtgag catttacccg ttccgcatga ccgacgccaa tatgaagcgc
     1321 cataaatatt ccaactttaa ggacttctgg gagcaactga agccaggcta cgactacttt
     1381 gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg gccgttacgt ggtcagcaag
     1441 ccgttaagcc acgaagttgt gcaaccacaa ctggcatcaa actacacgct ccccgaggca
     1501 aaataagcgc cattcgcctg gcataatctt ttgccaggtt tcattgcccg tcagcggctg
     1561 tgtcgcaatc accgtgacca catcattcgg tgtggtctgc gagctgaagt cgatttccac
     1621 atcctgatcc agcaacgttg ccacgccaaa cggcgcgcgg cgggtgatcc agtgcagatt
     1681 ggtcgagcaa tacgccatta catagcgccc atccgaaagc agcatgttga aaacgccctt
     1741 ctgccgcagt tcatccgcca gtgaggcgat atatttaaat accgccgcca tattgcccgg
     1801 cgtacgcgga taacgctgcg ttaatttata cagcagccag caaaaggctt tttcgctgtc
     1861 ggttttacct accgggcgga agttgccggt ttccagtgat ttatagcccg tcagttgtcc
     1921 gttatgggca taagtccagt tacgtcccca taactcgcgg gtaaacgggt gagtattttc
     1981 caacgccacc tcgccccgat ttgcctggcg aatatgagcc acaaccgaac aggattttat
     2041 cgggtagtcc tgaacaagtt tggcaatggg ggaattaaag ctgggttgtg gatctttaaa
     2101 tgtgcgacag cctttacctt cgtaaaaggt aatgccccag ccatctttat gtggcccggt
     2161 tccaccacca cgctgcacca gcccggtgaa actaaagcaa atatcggtag ggacgttggc
     2221 gctcatcccg agcaattcgc acatcggtaa cccccacaac agcgggagcg catggccccc
     2281 ggcgaagatt gacttaagaa ttttcaggcc ggatgcggcg taaacgcatt attcggccta
     2341 cgccagactt acttaaccat ctctttttca attaactgga tcaggatatg gatcacttta
     2401 atgtgaattt cctgaatgcg gtcggcataa ccaaaatgtg gaacgcgaat ttcaatatcc
     2461 gccgtaccag ccattttgcc gccatctttg ccggtcaggg taatcacttt cattcccttc
     2521 tcacgcgccg ccgcgatcgc tttgatcacg tttgcagagt taccagacgt ggagatcccc
     2581 agcagtacat cgccttcgcg gcctaccgct tcaacatagc gggagaaaat atcattgaaa
     2641 ccaaaatcgt taccgacgca ggaaatatgg ctgacgtcag agatggcaat cgccgggtag
     2701 cccggacggt tttcacggta gcgaccggtc agctcttcgg caaagtgcat ggcgtcgcaa
     2761 tgggagccgc cgttgccgca ggaaagcacc ttgccaccgg ctttaaagct gtctgctaac
     2821 aggaccgccg ctcgctgaat ggcgtgaata ttggcgtcat cttttaaaaa gttagccagc
     2881 gtttccgccg cttcgttcag ttcgttacga ataagatcct ggtacatgag gatatccttc
     2941 agcataaatg taatagacaa aatgcagtgt accggatacc gccaaaagcg agaagtacgg
     3001 gcaggtgcta tgaccaggac tttttgccct gaagtgtaga taaaaacagc aacaatgtga
     3061 gcttcgttgt aattatattg taaacacatt gctaaatgtt tttacatcca ctacaaccat
     3121 atcatcacaa gtggtcagac ctcctacaag taaggggctt ttcgttatga tgattttgag
     3181 tattctcgct acagttgtcc tgctcggcgc gttgttctat caccgcgtga gcttatttat
     3241 cagcagtctg attttgctcg cctggacagc tgccctcggc gtcgctggtc tgtggtcggc
     3301 gtgggtactg gtgcctctgg ccattatcct cgtgccgttt aactttgcac ctatgcgtaa
     3361 gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag gtgatgccgc cgatgtcgcg
     3421 cactgagaaa gaagcgattg atgcgggtac cacctggtgg gaaggcgact tgttccaggg
     3481 caagccggac tggaaaaagc tgcataacta tccgcagccg cgcctgaccg cagaagagca
     3541 agcgtttctc gacggcccgg tagaagaagc ctgccggatg gcgaatgatt tccagatcac
     3601 ccatgaactg gcggatctgc cgccggagtt gtgggcgtat ctgaaagagc atcgcttctt
     3661 cgcgatgatc atcaaaaaag agtacggcgg cctggagttt tcagcttatg cccagtctcg
     3721 tgtgctgcaa aaactctccg gcgtcagcgg gatcctggcg attaccgtcg gcgtgccgaa
     3781 ctcattaggc cccggcgagc tgctgcaaca ttacggcact gacgagcaga aagatcacta
     3841 tctgccgcgt ctggcgcgtg gtcaggagat cccttgcttt gcactgacca gcccggaagc
     3901 gggttccgat gcgggcgcga ttccggacac cgggattgtc tgcatgggcg aatggcaggg
     3961 ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc tacattacgc tggcaccgat
     4021 tgcgactgtg cttgggctgg cgtttaaact ctccgacccg gagaaattac tcggcggtgc
     4081 agaagattta ggtattacct gtgcgctgat cccgaccacc acgccgggcg tggaaattgg
     4141 tcgtcgccac ttcccgctga atgtgccgtt ccagaacgga ccgacgctcg gtaaagatgt
     4201 cttcgtaccg atcgactaca ttatcggcgg gccgaaaatg gccgggcaag gctggcggat
     4261 gctggtggag tgcctctcgg taggccgcgg catcaccctg ccttccaact caaccggcgg
     4321 cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac attcgccgtc agttcaaaat
     4381 ctctatcggt aagatggaag ggattgaaga gccgctggcg cgtattgccg ggaacgctta
     4441 tgtaatggac gctgcggcat cgctgattac ctacggcatt atgctcggcg aaaaacctgc
     4501 cgtgctgtcg gctatcgtta agtatcactg tacccaccgc gggcagcagt cgattattga
     4561 tgcgatggat attaccggcg gtaaaggcat tatgctcggg caaagcaact tcctggcgcg
     4621 tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg gctaacattc tgacccgcag
     4681 catgatgatc ttcggacaag gagcgattcg ttgccatccg tacgtgctgg aagagatgga
     4741 agcggcgaag aacaatgacg tcaacgtgtt cgataaactg ttgttcaaac atatcggtca
     4801 cgtcggtagc aacaaagttc gcagcttctg gctgggcctg acgcgcggtt taaccagcag
     4861 cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac ctgaaccgcc tgagcgccaa
     4921 cctcgccctg ctttctgatg tctcgatggc agtgctgggc ggcagcctga aacgtcgtga
     4981 gcgtatctcg gcacgtctgg gtgatatttt aagccagctt tacctcgcct ctgccgttct
     5041 gaagcgttat gacgacgaag gccgtaatga agccgatctg ccgctggtgc actggggcgt
     5101 acaggatgcg ctgtatcagg cagaacaggc gatggacgat ttactgcaaa acttcccgaa
     5161 ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg accggacgtc attatctggc
     5221 accgtctgac aagctggatc ataaagtggc gaagatttta caagtgccga acgccactcg
     5281 ctcacgtatt ggtcgcggtc agtacctgac gccaagcgag cataacccgg ttggtttact
     5341 ggaagaggcg ttggtggatg tgattgccgc cgacccgatt catcagcgga tctgtaaaga
     5401 gctcggtaaa aatctgccat ttacccgtct ggatgaactg gcgcacaacg cgctggcgaa
     5461 ggggctgatt gataaagatg aagccgctat tctggtgaaa gcggaagaaa gccgtctgcg
     5521 cagtattaac gttgatgact ttgatccgga agagctggcg acgaagccgg taaagttgcc
     5581 ggagaaagtg cggaaagttg aagccgcgta attgaataac ggagccgaaa ggctccgttt
     5641 ctttatccgc taattattta aaattaaagc catccggatg gttttccagg ctgccggtca
     5701 acgccgcgaa cagcaccgtt ttaccatcaa tcgaaagcgc atcgttcaca ttcaaccagg
     5761 tcagtttctc ttgcgacgtt ttctcatcaa tagtcgagaa cagccccgtc atctgattag
     5821 atttctcgga ccacatcaca gcgatacgtt gcgagccaca gtcatgcggt ttgcacgcgc
     5881 tcatcacctg atacgtctca tctcccaacg ttacggtttg tgcaggggta taagtaccgc
     5941 ctttcatcac ccaggcaggc agcttatgcc cttgcaccat ctgattaaat gcagctttgg
     6001 tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc ctgcgccatt gcactggtgg
     6061 cgatgaccag tgcggcgact gtcgttattg ccttaaacat cattcctccc gagcttatcc
     6121 tgcccatgtt attaacctcc taaagcctgg cgataacacg ataatttgca aatcagatat
     6181 ttccaatatc agcatttgtt atcaatttat tctgcgctaa ggcaactgcc tgataaggtg
     6241 ttaaagttat aaaacccttc gcataaagga gttgtacacc gtgcctggtt tgaagattac
     6301 gcttttgcag caaccactgg tgtggatgga tggtcctgcc aacctgcgtc attttgatcg
     6361 tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta ccggagatgt ttaccagcgg
     6421 ctttgccatg gaagcggctg cttcgtcgct agcacaagat gacgtagtga actggatgac
     6481 agccaaagcg cagcagtgca atgcgctgat tgcaggcagt gttgcattac aaacggagtc
     6541 tggttcggtt aaccgctttt tgctggttga gccgggcggc acggtacatt tttatgataa
     6601 gcgtcatctg ttccgcatgg cagatgagca tctgcattat aaagcgggca atgcgcgagt
     6661 gattgtggaa tggcgcggct ggcgtatttt gccgttggtg tgctacgact tacgttttcc
     6721 ggtgtggtcg cgcaatctca acgattatga cctcgccatc tacgttgcca actggcctgc
     6781 tccgcgctct ctgcactggc aggcattgct gacggcccgc gcgattgaga atcaggcgta
     6841 tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc tgccattatc gcggtgacag
     6901 ccgggtgatt aatccacaag gcgagattat cgctactgcc gacgcgcatc aggcaacgcg
     6961 cattgatgcg gagctgtcga tggtggcatt gcgggaatat agagaaaagt ttccggcatg
     7021 gcgggatgcg gatgagttta ggttgcggtg agtgggggct gtatttaggg cggggaattc
     7081 agaaagggat gtttagtgtg ctgagcgaga gttaaataat aaaaaactgg gaagatttaa
     7141 taatcccccc gcagggcaag agcatgaatt aaatctgtgt taaatttttc attttgtcag
     7201 ttcgaaaccc aagctctgag caggatgcaa gcacttcttc tatataacat gtgtttaacg
     7261 caaccttctt tcttttcctc ttcaccccac ccttaatgtc tttgcaatct cttaataaat
     7321 tcagtgccat cttctttatt ccagatatta tttcggctgc gtttcctctt cttatccggc
     7381 tggcatcttc attcattttt acatctaaca cccaatgaag actgtgctcg atcagccagt
     7441 gcgctctgat agcatgtgca aattctttag catccatatc ctttgatgaa atataatatc
     7501 ggatgcttac accttctgct gatttatctt ctttcttctg cctgaatgac aatgctacac
     7561 aaagcttttt taatcccttc cattcgaatt caaaatcaca aaattcaggc gttacgttac
     7621 tgacaatatg caaacgtgtt tcttttcttc catgacttat ctcctgcgta ctaaacgaat
     7681 cgcctttata attagaaaac acatttacag gaaatttttc ctcgaatgca tgatgtaatt
     7741 tcccctgatt gccttttact gccagaagat aatctgcttt tttatctttg atcttcgaag
     7801 cgatatcttt ctgacagccc atagcatcaa tggttatcaa atttttcttt aaattcagta
     7861 ggttaagcaa ctctggaatg gctgtaatct cattactttt ggcttccgtt tttacctgcc
     7921 ccagtacaac accattttcg ttcgagaatg cactcaccat atggattgct ccttttcttt
     7981 ttcccttatc aaaggagcct cttatggtct ttccatctat tgctataatt tcaccatcag
     8041 tgatttcatg gcactcctgc atccattcaa taaatatctt ttcaaaggcc aaactgtcaa
     8101 tgttactcac aacgcgtgca atggtgtcat cgaccggaat gccattatca aaatcaccat
     8161 atttctttag ccattcaagt ctttcatgtc caaaatcttc aatttcctgc cactcatcgg
     8221 cacctgcaat tactgcacat acggtgagaa acaaaatatc agataattta tgtttaacct
     8281 ttccttgttg tcgtatatca gggatcactg aaatataatc aagcaaactt tgaatactca
     8341 cctggcgtcc tttttatctt tttagttatt ccaaaggtta aattttgatc acctgtaggc
     8401 gttaaagcca ggatgaaaat cgtgatttac atcacgattt tcatgatctt gccctgcccc
     8461 gtcccttgct c
//
LOCUS       DAZFTN010000167         8392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFTN010000167 DAZFTN010000000
VERSION     DAZFTN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(171..247)
                     /locus_tag="ACHKV2_004586"
     tRNA            complement(171..247)
                     /locus_tag="ACHKV2_004586"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Asp,seq:gtc)
     gene            complement(380..1111)
                     /gene="dnaQ"
                     /locus_tag="ACHKV2_004587"
     CDS             complement(380..1111)
                     /gene="dnaQ"
                     /locus_tag="ACHKV2_004587"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO6942337.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1176..1643
                     /gene="rnhA"
                     /locus_tag="ACHKV2_004588"
     CDS             1176..1643
                     /gene="rnhA"
                     /locus_tag="ACHKV2_004588"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO6942338.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1640..2362)
                     /locus_tag="ACHKV2_004589"
     CDS             complement(1640..2362)
                     /locus_tag="ACHKV2_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6942339.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2396..3151
                     /gene="gloB"
                     /locus_tag="ACHKV2_004590"
     CDS             2396..3151
                     /gene="gloB"
                     /locus_tag="ACHKV2_004590"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO6942340.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3223..4581
                     /gene="mltD"
                     /locus_tag="ACHKV2_004591"
     CDS             3223..4581
                     /gene="mltD"
                     /locus_tag="ACHKV2_004591"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO6942341.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4629..5399)
                     /locus_tag="ACHKV2_004592"
     CDS             complement(4629..5399)
                     /locus_tag="ACHKV2_004592"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6942342.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5477..6277)
                     /locus_tag="ACHKV2_004593"
     CDS             complement(5477..6277)
                     /locus_tag="ACHKV2_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6942343.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6518..7432
                     /gene="yafC"
                     /locus_tag="ACHKV2_004594"
     CDS             6518..7432
                     /gene="yafC"
                     /locus_tag="ACHKV2_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO6942344.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7429..8232)
                     /gene="dkgB"
                     /locus_tag="ACHKV2_004595"
     CDS             complement(7429..8232)
                     /gene="dkgB"
                     /locus_tag="ACHKV2_004595"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO6942345.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2238 a         2000 c         2048 g         2106 t
ORIGIN      
        1 gaatggttcg atatcattat tgaattttaa tgaatgagac aacaagcgag aatcacgcac
       61 ttacgccaga gtaaaatcat tgaacagaga aatggtggaa ttcaaataca aaaaaaaccg
      121 caaaattaaa aatcttgcgg ttctctgaac tcattttcat gaggtgaatt tggcggaacg
      181 gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta
      241 ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt acggattgcc tcacccttcg
      301 tcaacggttt ttctcatctt ttgaatcatt tgctgcaaaa atcgcccaag tcgctatttt
      361 tagcgccttt tacatgtatt tatgcccgcc agaggcagct tccgcccttt ttctgcacca
      421 gatcgagacg ggcttcatgt gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact
      481 tacttgcctg acgtacaagg cgctggattg ttgcttcacc ttgttgctgt tgtgtctctc
      541 cttccatcgc aaaagccatc gacgtttgac caccggtcat cgccagataa acttccgcaa
      601 ggatctgggc atcgagtaat gccccgtgca gcgttcgttt actgttatct atttcgtagc
      661 gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa cattttcctc gccaccgcaa
      721 ggctatcggt gaccttacag aaggtattag tcttcggaat atcgcgctta agcaacgaga
      781 actcgtagtc cataaagccg atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc
      841 ggatatagtc catgaactca tcggctactt cggcaaacgt gggcttatcg agcaaaaatt
      901 catcggcaat accatgtacg ccaaaggctt ccggatccac cagccgatcg ggtttgagat
      961 aaacatggaa gttattgccc gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa
     1021 tgatcttgtg gccttcatag tgcgcaccaa tctggttcat accggtggtt tcggtatcga
     1081 gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat ttatgtcaga cttgtcgttt
     1141 tacagttcga ttcaattaca ggaagtctac cagagatgct taaacaggta gaaattttca
     1201 ccgatggttc gtgtctgggt aatcctggac ctgggggtta cggcgctatt ttacgctatc
     1261 gcggacgcga gaaaaccttt agcgcaggct acacccgcac caccaacaac cgtatggagt
     1321 tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca ttgcgaagtc attttgagta
     1381 ccgacagcca gtatgtccgc cagggtatca cccagtggat ccataactgg aaaaaacgtg
     1441 gctggaaaac cgcagacaaa aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg
     1501 ctgcattggg gcagcatcaa atcaaatggg aatgggttaa aggccatgcc ggacacccgg
     1561 aaaacgaacg ctgtgatgaa ctggctcgtg ccgcggcgat gaatcccaca ctggaagata
     1621 cgggctacca agtcgaagtt taagcctgtg gtttacgaca ttgccgggtg gctccaaccg
     1681 cctggcgaat tcgtggcttg tttttactct gtttcatcgg atttagcgtt aaaggaatag
     1741 tccgtttccg ggcaacaata agttgtaagc aaccaagcgc aggaatatgc gcattcaaaa
     1801 gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg gctggcgtgt agcacttcaa
     1861 aattcaacaa agagagccag tccagttgcc gcatcagagt aaacatccgg ctgttatagg
     1921 gcgaggtttt gcgcaatacc ggcacaagtt tgcgtaatcc cattaaacta atgggattga
     1981 agccactaat gaccagccag ccatcatcaa tcaatacccg atcggcttca cgcaataaac
     2041 gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag acaaacatca acggatttat
     2101 cggcaaaagg aagatgaagt gggtccgcct gcacctggac aggcattcct tgcgcagaaa
     2161 cattcacttg atgagaaacc gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa
     2221 tcttaagcag ataaaaacca tacattttag tgaaccacgg gttgagctgg cgctccagcg
     2281 ccttgcgata aagctctccc cagggcaaat cgccccagcg atcaggagcc acgacagttt
     2341 gagggactct tgccggtttc atcacaacct tccgtttcac actgagaggt aatctatgaa
     2401 tcttaacagt attcccgcct ttgatgacaa ttacatctgg gttttgaatg atgaagcagg
     2461 tcgctgcctg attgtcgatc ccggagacgc agagccagta ttaaacgcca ttgccgccaa
     2521 taactggcaa ccggaggcca tatttctcac ccaccatcat cacgatcacg ttggcggcgt
     2581 aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat ggtccacaag agacacaaga
     2641 taagggaaca acacaggtag tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt
     2701 tagtgtaatt gccacgccgg gtcacacttt aggacatatc tgttacttca gtaaacctta
     2761 tctattttgc ggcgacacgc tgttttctgg tgggtgtggt cggttgtttg aagggacagc
     2821 atcacaaatg tatcaatcac ttaaaaagtt aagtgcgtta cctgacgata cattggtatg
     2881 ttgtgctcat gaatatacgt tatcaaatat gaagtttgct ttgagtattc ttccgcacga
     2941 tttgtccata aatgattatt atcgtaaagt taaggagtta cgggcaaaaa atcaaataac
     3001 actacccgta attctgaaaa atgagcggca aattaatgtt tttttaagaa cggaagatat
     3061 tgatttaatt aatgtaatta atgaagaaac attattgcaa caacctgaag agcgttttgc
     3121 atggttaagg tcaaagaaag ataggttctg ataaaacttt cttgtcatcg gctccgttcg
     3181 ccgttatgat cggtcgtctt ttaagcaact attgacgcac acatgaaggc aaaagcgata
     3241 ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg gcaacgttca acagcacgca
     3301 cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt ttacaagtca ggcacgatgg
     3361 atggacgatg ggacgtctat cgcgccagat ggtgacttgt gggctttcat tggcgacgag
     3421 ctaaagatgg gaattccgga aaatgaccgg attcgcgaac agaaacagaa atatttacgc
     3481 aataagagct atctccacga tgtaacttta cgggcagagc cgtatatgta ctggatagcc
     3541 gggcaagtta aaaaacgtaa catgcctatg gaactggtac tactacccat agtggagagc
     3601 gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag gcatctggca gatcattccg
     3661 agcacggggc gcaattatgg tttgaaacag acccgcaatt atgacgcgcg tcgcgatgtt
     3721 gttgcttcaa caactgccgc gctgaatatg atgcagcgtc tgaacaaaat gtttgatggc
     3781 gactggcttc tgaccgtagc ggcttataac agcggcgaag gtcgggtcat gaaggcaatt
     3841 aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt cgttaccgtt gccgcaggaa
     3901 acgaagcagt acgtgcctaa aatgctggca ttgagtgata ttctcaaaaa cagcaagcgt
     3961 tatggcgtac gtctgccaac gaccgatgaa agccgtgctc tggcgcgtgt gcacctgagt
     4021 agcccggttg aaatggcgaa ggttgcagat atggcgggga tttccgtcag caagctgaag
     4081 acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa gtggtccgca gtacgtgatg
     4141 gtgccaaaga agcatgcaga tcaactgcgt gaatctctgg cttcaggcga aattgctgct
     4201 gtacagtcga cgctggttgc cgacaatacg ccgcttaaca gccgtgttta caccgtacgc
     4261 tctggcgaca cgctttcaag tatcgcttca cgtctcggcg taagcaccaa agatttgcag
     4321 cagtggaaca aactgcgcgg ctctaagctg aaaccaggcc aaagtctgac gattggcgca
     4381 ggtagtagcg cacagcggtt ggcaaacaac agcgatagca ttacgtatcg tgtgcgcaaa
     4441 ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga acatcaaaga tgtgatgcgc
     4501 tggaacagcg atactgcgaa tctgcaacca ggcgataagc tgacgttgtt tgtgaaaaac
     4561 aacagcatgc cagactcctg acaaaccaga taatagaaag gcaccgattc ccccggtgcc
     4621 ttttttattt atgccgcttt atgtgcatct accatgatga tatcactggt gaaagagcca
     4681 tcattctgca aggcaaaata cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga
     4741 atagcgtcta ctaacgcttc tggcgtacgc attctcgcga cccatgaaga aaattccagc
     4801 ggtaacttat ctgtaattaa attatcaact atcagattgg cttcattgat taacctcaac
     4861 cactcaccgc tggcgtagtt tcgtacgtga gaggtatcgc ataatgcttc taccgtctgt
     4921 aaccagatgt cgcgcactgg gtggcccgga gacattacgt ccatcacaat cagcctaccg
     4981 ccaggtttca atatccggtt cacttcacgc agtgctgcac caacatcatg ccagtgatgg
     5041 gcagaataac ggctgataac aatatcaaat gcgttatcgg caaatggcag actttcggca
     5101 tacccctggc gggtggtgat atttttcagt tgccggactt cggcagcttg tgccacgaca
     5161 tccagcattt gggcagataa gtcatacgcc accaccgtgc tcacattttg cgcagcgaca
     5221 aagctggcat gccctgctcc gcagcccata tcaagtacac ttgcatcagg ataatcagcc
     5281 agacacaccg ccaggagctg taaatctcgt ccggatgcat gtacggtact ggtcagatat
     5341 tcgctggctt gagagctaaa ctgcttttct acgtggtcat ggtgggattg tgttgtcatt
     5401 gcactgtcct tttgttgttt gagtataaaa gggcagctcc aaacgttggt ctgccctgtg
     5461 gcagacctga cataccttat ttatcaggct tgccgggact gaattcaacg agtagcggat
     5521 tgtgatcgga cgcgcgcgta accagtacag aagcttcgct gacgttcaga ccacggtaga
     5581 aaacaaaatc gagcgggcga ccaaacgccc gacggcgctg atcatcggta aaacgcacct
     5641 gacgcagcga catttcccgc gcaaaacgat ataacgcgtt catccttcta cggctccaag
     5701 cattgaaatc tcccgccata atgaccgggc cgctgtggtg agctatctga tcaccaatag
     5761 gaagtaactg cttactataa acatccacgc ccagactgaa gttgacggcg tgtatattaa
     5821 ccaccattaa caggcgggtg tcaggtaatg gatagaccgt caccagtgcc gacttcgcca
     5881 gacgcaaaat gggttctcgt tcacgtaacg ggcagcaata cactggatgt gccgccgaaa
     5941 gggtcattac gccagaagga tgttgtggca gcacgaaagc gggtacctga tcggcggcaa
     6001 gatagttagc agtcgcaaac tgtaccaact ctggcgttgt ttgcgcttcc tgcaataaca
     6061 ccagatgtgc gtctttgccg taattcttta ataccgacaa ccattcagcg cgttgctgtt
     6121 tgtatatgtt ccacaccagg atccgaatac gctcttcggt acttaacggt tctccgggtg
     6181 gtaatgcctg gccgatgctc gcaaaagacc ccggcggtaa gatcctctcc gcaggttgtc
     6241 cggcaacata gcgcatggca taggtgtttt ttcgcacttt tgactttgaa acctctatta
     6301 cgtcaaccct tccgcactcg ggagggttct actgttatag ggattttagc tcactctttc
     6361 aacgagcaat tgtatattgt tatgtaagca agtgcttaca ccggtcagaa ttttgctcat
     6421 tattgagaac attaaatcgg atgacatatc acgcatcctg atggtttaat gacaaataaa
     6481 ttcaaaatat ctcatctatt ttgcaacagg agcaaaaatg aaagccacgt cggaagaact
     6541 cgccattttt gtttcggtcg tcgaaagcgg cagctttagc cgggcagcgg aacaattagg
     6601 gcaagcaaac tcagcggtaa gccgggcggt gaaaaagctg gagatgaaac ttggcgttag
     6661 cctgcttaat cggaccacgc ggcaacttag cctgacggaa gaaggcgagc gttattttcg
     6721 tcgcgtacag tcaattttgc aggagatggc agcggcagaa tcagaaatta tggagacgcg
     6781 taatacaccg cgtggactgt tgcgcatcga tgccgcaact ccagtggtgc tgcactttct
     6841 gatgccgttg attaagcctt ttcgtgaacg ctatccggaa gtcactttat cgctagtctc
     6901 ctccgaaacg attattaatt tgatcgaaag aaaagtggat gtcgcgatac gcgccggtac
     6961 gttaacggat tccagcttac gtgccaggcc gttatttaac agttatcgaa aaattatcgc
     7021 ctcccccgat tatatttccc gctacgggaa gccagaaacg atcgacgatt taaagcaaca
     7081 tgtttgcctg ggattcactg aacccgcttc cctcaatacc tggccggtag cctgtagcga
     7141 tggacaatta catgaggtga agtacggttt gtcatccaat agtggggaaa cactgaaaca
     7201 gctttgcctg agtggcaacg ggattgcgtg tttgtccgac tatatgatcg acagagaaat
     7261 cgctcgcgga gaattggtgg agttaatggc agataaagtg ttgccagtgg aaatgccatt
     7321 cagtgccgtc tattacagcg accgtgcggt aagtacgcgc atccgggctt ttatcgattt
     7381 ccttagcgag catgtaaaaa cagctcccgg aggagctgtc agagaggctt aatcccattc
     7441 aggagccaga ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt
     7501 tttatcttcg gcatcaagct gtaaattttg tgccttaaga ttactctcca ggttttcacg
     7561 tttagtagaa gaaggaatta ctgagtaacc ttcccccata gcccacgcca gaatcacttg
     7621 tgccggagtc gcattgtgtt tagctgcgat acgagcaata acctcatctt tcagggcctt
     7681 accatacgcc agcgtcatat aggaagtaat atggatgccg tgctgtttag cccaggcaac
     7741 cactttacgg ttttgcagat aaggagagag ttcaatctgg ttagtagcga tgttttcagc
     7801 gccaacagca gcaatcgcct tttccatcaa tgggatcgtg aagttggaaa taccgatctc
     7861 acgcgtcaac ccttcttttt tggcttccag cagcgcctgc ataaactctt caacagagac
     7921 ttcatcgttt ggtgacggcc agtggattag cgtcagatca acataatcgg tacgcaattt
     7981 ttgcaggctc tcttttagac tcgggatcaa tttgtctttg ctgagatttt caatccagat
     8041 tttagtggtg atgtagagtt catgacgtgg cacgccactt tctgcaatcg ccagacctac
     8101 tgcggcttcg ttatcataga tttgtgcagt atcaattgcg cgataaccaa gttcaagcgc
     8161 cgttttcaca gatgaaataa caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc
     8221 agggatagcc ataatatgcc tcttttaatg ctattttcgt taactgaggt gattatgggc
     8281 tttagaatgt tgcggaaaaa gggcgtaaaa cgcagaggat ttttgcgatt ttagcaataa
     8341 tagaaatgta gacgaaaaaa aacctcgagc gataactcga ggcttcttaa ta
//
LOCUS       DAZFTN010000168         8366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFTN010000168 DAZFTN010000000
VERSION     DAZFTN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(96..302)
                     /locus_tag="ACHKV2_004596"
     CDS             complement(96..302)
                     /locus_tag="ACHKV2_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO6942346.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            615..704
                     /gene="kdpF"
                     /locus_tag="ACHKV2_004597"
     CDS             615..704
                     /gene="kdpF"
                     /locus_tag="ACHKV2_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO6942347.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            704..2377
                     /gene="kdpA"
                     /locus_tag="ACHKV2_004598"
     CDS             704..2377
                     /gene="kdpA"
                     /locus_tag="ACHKV2_004598"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO6942348.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2400..4448
                     /gene="kdpB"
                     /locus_tag="ACHKV2_004599"
     CDS             2400..4448
                     /gene="kdpB"
                     /locus_tag="ACHKV2_004599"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO6942349.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4457..5029
                     /gene="kdpC"
                     /locus_tag="ACHKV2_004600"
     CDS             4457..5029
                     /gene="kdpC"
                     /locus_tag="ACHKV2_004600"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO6942350.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5022..7706
                     /gene="kdpD"
                     /locus_tag="ACHKV2_004601"
     CDS             5022..7706
                     /gene="kdpD"
                     /locus_tag="ACHKV2_004601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO6942351.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVEPPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7703..>8366
                     /gene="kdpE"
                     /locus_tag="ACHKV2_004602"
     CDS             7703..>8366
                     /gene="kdpE"
                     /locus_tag="ACHKV2_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO6942352.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGY"
BASE COUNT         1753 a         2186 c         2436 g         1991 t
ORIGIN      
        1 ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg ccagcgtttc
       61 cgctggcggt tttcaatgta ttcaaaactc agcaatcagt aaaaatcacc ggttgccttt
      121 tcggcctgat ccatccaaac cggtttatcg ctggttttcg accagacgcg atgcaggtag
      181 ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag aacgcccgct
      241 gcaaccgcat aagtacggcg taaaaagata agccatgcag gatattctct gtagagttcc
      301 atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat gaatttgttt
      361 atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac cacttatttt
      421 tgcttattga tggtttattt acattcaccc tgtaattaag ttacacaaaa gttaaattaa
      481 tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta caccccgtcc
      541 gcagattttt gcgaaatctt tgcagccaga attctaccct tccggtatca cttttaggcc
      601 actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg tttttattac
      661 tgggttattt ggtttatgcc ctgatccatg cggaggcatt ctgatggctg cgcaagggtt
      721 cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtccct taggtagcgg
      781 gctagcgtgg ctgattaatg atattcctct tcctggtacg acgggcgttg aacgcgtact
      841 ctttagcgcg cttggcgtct ctaaccatga gatgaactgg aagcagtatc tctgtgccat
      901 tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg gtcagcacta
      961 tctgccgctt aatccacaac agttgccggg gctgtcgtgg gatctggcgc tgaataccgc
     1021 cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca cgttgagcta
     1081 tttcagccag atggcgggct taacggtgca aaactttctt tctgccgcca gcgggattgc
     1141 ggtgattttt gccctcattc gtgcatttac ccgccagagc atgagcacac tcgggaatgc
     1201 ctgggtcgat ctgctacgca tcacgttatg ggtgctcgtc cctgtggcgt tggtgatcgc
     1261 actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg cagtgaatac
     1321 cgttgaagga gcgcaacagc tgttaccaat gggtccggtg gcttctcagg aagcgatcaa
     1381 gatgctcggc actaacggcg gtggtttctt taatgccaac tcgtcgcatc cgtttgaaaa
     1441 cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc caacagcgct
     1501 gtgctttgcc tttggtgaag tgacgggcga tcgccgtcag gggcgcatgt tgctgtgggc
     1561 gatgtcagtg atttttgtca tctgcgtagg cgtggtgatg tgggcagaag ttcagggtaa
     1621 tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta aagagagccg
     1681 tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc
     1741 ggtgattgca atgcatgatt cgtttaccgc tctcggtggc atggtgccga tgtggctgat
     1801 gcaaattggt gaagtggtat tcggcggtgt cggttctggt ctttacggca tgatgctgtt
     1861 tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg aatatctggg
     1921 taaaaaaatc gacgtacgtg agatgaaact gactgcactg gcgattctgg tcaccccgac
     1981 gctggtgctg atgggcgcgg cgctggcaat gatgaccgac gccggacgta gcgccatgct
     2041 caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcatccg ccgctaacaa
     2101 caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact gtttactggc
     2161 gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa ttgccggttc
     2221 gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc acggcccgct
     2281 gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct ttatccctgc
     2341 cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagtga gcactgaata
     2401 tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg ctgaaagaag
     2461 cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga
     2521 tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggcgcg atgcctggca
     2581 atgcgctgtt tagcgtggcc attagcggct ggctgtgggt caccgtactg ttcgctaatt
     2641 tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagcctg aaaggggtga
     2701 aaaaaacggc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg gcggacaaag
     2761 ttcccgccga tcaactgcgt aaaggcgata tcgtactggt agaagcaggc gatattatcc
     2821 cctgcgatgg tgaagttatt gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg
     2881 aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc
     2941 gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggcgag acatttctgg
     3001 atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacgccgaac gagattgccc
     3061 tgaccatcct gctgattgcc ctgactatcg tctttttact ggcaaccgcc acgctgtggc
     3121 cgttttccgc gtggggcggt aatgcagtca gcgtaacggt actggtggcg ctgctggtct
     3181 gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc gggatgagtc
     3241 ggatgctggg cgcgaatgtg gttgccacca gcggacgtgc agttgaagcg gcaggtgacg
     3301 ttgacgttct gctgctggat aaaaccggca ccatcacact cggtaaccgt caggcatcgg
     3361 agtttatccc tgcgcagggc gtggatgaaa aaacgctggc tgacgccgca caactggctt
     3421 cgctggctga tgaaacgccg gaaggccgca gtattgtgat cctcgccaag cagcgtttta
     3481 acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa
     3541 gccggatgag cgggatcaat atcgacaacc gcatgatccg taaaggttct gtcgatgcca
     3601 ttcgccgcca tgttgaggct aacggtggtc acttccctgc cgatgttgat caaaaagtcg
     3661 atcaggttgc gcgtcagggg gccacaccgc tggtagtagt agaaggttct cgtgtgctgg
     3721 gcgttattgc gttgaaagat atcgtcaaag gcggcattaa agaacgcttc gcccaactgc
     3781 gcaaaatggg tattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     3841 tagctgcgga agcgggcgtc gatgattttc tcgccgaagc gacaccggag gccaagctgg
     3901 cattgatccg tcagtatcag gcggaaggcc gtttggtagc aatgaccggc gacggcacca
     3961 acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc ggtactcagg
     4021 cggcgaaaga agcgggcaat atggtggatc tcgactctaa cccgaccaag ttgatcgagg
     4081 tggtacatat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     4141 ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc attcgcggta acgtatccac
     4201 agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg
     4261 tcattttcaa cgccttgatt atcgtctttt tgattcccct ggcgttaaaa ggcgtgagtt
     4321 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     4381 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actactgacc gtttgcggtc
     4441 tggtgtgagg tttaccatgc gtggattacg tccggcatta tcgacattta tctttctgtt
     4501 attgattact ggcggtgttt acccgctgct gaccaccgcg ctggggcaat ggtggtttcc
     4561 ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcggggtt cggcattaat
     4621 cgggcagaat tttaccgaca acggctattt tcatggtcgc ccgtcggcaa cggcagaaat
     4681 gccttataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc ctgagctgga
     4741 taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccgaatg ccagcacgag
     4801 cgttccggtt gaactggtga cggcgtcggc aagcgggctg gacaataaca tcaccccgca
     4861 agcggcggcc tggcagatcc cacgcgtagc gaaagcgcgt aatctcagcg ttgaacagct
     4921 cacgcaactg atcgcaaaat acagccaaca accactggtg aaatatatcg gccagccggt
     4981 cgtcaacatt gtcgaactca atctggcact agataaactt gatgaataac gaacccttac
     5041 gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     5101 ttttcttcgg tgcctgcgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     5161 gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac gggcggaaag
     5221 ataccgccgc catgctggaa gggctggctg ttctgccgcc aaaacgcctg gcgtaccgtg
     5281 ggcggcacat cagcgagttt gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct
     5341 taatggacga actggcgcac agtaatgcgc caggttctcg tcatcctaaa cgctggcagg
     5401 atatcgaaga actgctggaa gctggcattg atgttttcac aaccgtcaac gttcagcatc
     5461 tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc
     5521 ccgatccttt tttcgatgcc gccgacgacg tggtgctggt ggacttgccc ccggacgatc
     5581 tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag cgcgccattg
     5641 aacatttttt ccgcaaaggc aatctgatcg ccctgcgcaa actggcactg cgccgtactg
     5701 ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct
     5761 ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggcagc gaaaaactgg
     5821 tccgcgcggc ggcgcggctg gcgtcacggc tggggagcgt ctggcacgcg gtgtatgttg
     5881 aaccccctgc cctgcatcgc ttaccggaga aaaaacgtcg ggcaattctc agcgccttac
     5941 gtctggcgca ggagctgggc gcggagacgg caacactttc tgatccagcg gaagagaaag
     6001 cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt cgcccagcct
     6061 cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc gcccccgatc
     6121 tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac aacgcgccgg
     6181 atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg gttgccgccg
     6241 cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt gatgccgcca
     6301 acctggtgat gctgtatcta cttggcgtgg tggtggtggc gctattttat ggacgctggc
     6361 cttcggtggt tgctaccgtc attaatgtgg tgagtttcga tctctttttt atcgccccac
     6421 gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg atgttaaccg
     6481 tcgggctggt gatcgggaac cttactgcgg gtgtgcgtta tcaggcgcgg gtagcccgtt
     6541 accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg gtgggccgca
     6601 gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt catgcccgca
     6661 gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat ccgcaaggaa
     6721 tgacgccgtg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg cctgcgggtg
     6781 cgggcaccga cacgttaccc ggcgtgccgt accagatttt gccgttaaaa agcggtgaga
     6841 aaacctacgg gctggtggtg gtggaaccgg ggaatctgcg tcagttgatg atcccggaac
     6901 agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcgcttgag cggctgacgc
     6961 tgaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     7021 tgctggcggc gctttcgcat gatttacgca ctccgcttac ggtgctgttt ggtcaggcag
     7081 aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag gccagcgaga
     7141 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     7201 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     7261 gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     7321 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     7381 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg
     7441 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca ggccaggagc
     7501 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcagtaccg ggggtagggc
     7561 ttggactggc aatttgtcgg gcgatagtgg atgtacacgg gggcactatt accgcgttca
     7621 accggccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaact gcccctgaac
     7681 ttgaagaatt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     7741 tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctatg aggccgaaac
     7801 gctgcaacgc ggcttgctgg aagcggctac ccgtaagcca gatttgatta ttctcgatct
     7861 cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcgcggt
     7921 gccggtgatt gtgctttccg cacgcagcga agagagcgac aaaatcgccg cgctggatgc
     7981 cggagcggat gattatctaa gtaagccgtt tggcattggc gaattgcagg cccgtctgcg
     8041 cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga aattttccga
     8101 tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac
     8161 cccgattgag tttcgcctgc tggcagtgct gctcaacaac gccggaaaag tgctcaccca
     8221 gcgccaactc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc attatttgcg
     8281 tatttatatg ggacatctgc gacaaaaact ggaacaggat cccgctcgcc ccagccattt
     8341 cattactgaa accggtattg gttatc
//
LOCUS       DAZFTN010000169         8120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFTN010000169 DAZFTN010000000
VERSION     DAZFTN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..269
                     /locus_tag="ACHKV2_004603"
     CDS             <1..269
                     /locus_tag="ACHKV2_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942353.1"
                     /translation="RNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYK
                     SRHEMLPVNLTPETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            492..989
                     /gene="ubiC"
                     /locus_tag="ACHKV2_004604"
     CDS             492..989
                     /gene="ubiC"
                     /locus_tag="ACHKV2_004604"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO6942354.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1002..1874
                     /gene="ubiA"
                     /locus_tag="ACHKV2_004605"
     CDS             1002..1874
                     /gene="ubiA"
                     /locus_tag="ACHKV2_004605"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO6942355.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2029..4452)
                     /gene="plsB"
                     /locus_tag="ACHKV2_004606"
     CDS             complement(2029..4452)
                     /gene="plsB"
                     /locus_tag="ACHKV2_004606"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO6942356.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4623..4991
                     /gene="dgkA"
                     /locus_tag="ACHKV2_004607"
     CDS             4623..4991
                     /gene="dgkA"
                     /locus_tag="ACHKV2_004607"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6942357.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5101..5709
                     /gene="lexA"
                     /locus_tag="ACHKV2_004608"
     CDS             5101..5709
                     /gene="lexA"
                     /locus_tag="ACHKV2_004608"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO6942358.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5782..7107
                     /gene="dinF"
                     /locus_tag="ACHKV2_004609"
     CDS             5782..7107
                     /gene="dinF"
                     /locus_tag="ACHKV2_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO6942359.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7223..7432
                     /gene="yjbJ"
                     /locus_tag="ACHKV2_004610"
     CDS             7223..7432
                     /gene="yjbJ"
                     /locus_tag="ACHKV2_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO6942360.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7474..7989)
                     /gene="zur"
                     /locus_tag="ACHKV2_004611"
     CDS             complement(7474..7989)
                     /gene="zur"
                     /locus_tag="ACHKV2_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO6942361.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1823 a         1992 c         2235 g         2070 t
ORIGIN      
        1 cacgaaacga taatatcttt atcttgttct cctctgtcga tgatgggcca gttatggtgg
       61 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      121 cgtatatcta taaatccaga catgagatgt tgcctgttaa tcttactccg gaaacacttt
      181 tcggctccaa atcttatgat aaacatgcgc ttttccccat ttttactgcg agttggcgag
      241 ctaatcgtat aaagaataaa ggtatttaag taaaggaaaa catcaccgct cttggcatca
      301 tgggcggtga tgcccctatg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt
      361 caacagcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa
      421 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      481 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattgtaaag
      541 agatccctgc cctggagccg caactgctcg actggctgtt gctggaagat tccatgacaa
      601 aacgttttga acagcaggga aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg
      661 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
      721 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
      781 tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac
      841 gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg
      901 gactgtgggg gcgacgttcc cgcttgcgat taagcggtaa accgctgttg ctaacagaac
      961 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1021 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1081 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt ccccagctct
     1141 ggatcctggc ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga
     1201 atgattatgc tgaccgcaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1261 ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt
     1321 cgtttttact ggtgctgacg ctgaatacga tgaccattct gttgtcgatt gccgcgctag
     1381 cgctggcgtg ggtgtacccg tttatgaagc ggtataccca tctaccgcaa gtggtgctgg
     1441 gcgcggcgtt tggctggtcg attccaatgg cttttgctgc tgtgagtgag tcggtgccat
     1501 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1561 atgcgatggt tgaccgtgat gatgatgtga agattggcat taaatccacc gcaatcctgt
     1621 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcgctgatgg
     1681 cgattatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt ctggtggctg
     1741 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgcgaa gcctgcttta
     1801 aagcatttat gaataataac tatgttggtc tggtactgtt tttagggctg gcaatgagtt
     1861 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     1921 tgatgcgctg cgcttctcag gcctacacaa cagatcgcaa tttattgaat ttgcagatta
     1981 tggaaggccg gataaggcgt ttgcgccgca tccggcaatt ctctctgatt acccttcgcc
     2041 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2101 ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc
     2161 acgcagcgta agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2221 gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg
     2281 gttgatcgac gggttggcgc tcaacagcca gaaggtgatg gcataacgtt gcagcgtttc
     2341 gcgcgcgcct gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc
     2401 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2461 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2521 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc
     2581 cgccatcagc gaaggtagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     2641 caccgcttgc tcgcgcggca gaatgatgat gtcgccgata gtgtctttct caacttcaaa
     2701 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     2761 gtccgtggag taaggcacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     2821 ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat
     2881 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     2941 cgtcggcgtt aaccatgccg gacgcactgc ttcgatggga tcgatagatt cacgccagtc
     3001 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaaat tgacgtaacc
     3061 ctggccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3121 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3181 gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3241 catcgacagc gtaccggttt tcggatccag caaacgcccc gtacgggaac gaccgccttc
     3301 tacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3361 ggtggaatag agcttattgc ctttaaaagt acggcgaata aagaacgcac ccagacggcg
     3421 gaaaatcggc ccggcaggcc agaaattcag gttgatcccg gcggcgatat gcggcggcac
     3481 tagcccctgg tggtacagca cgtaagaaag cagcaggtag tccatgtgac tgcggtggca
     3541 aggcacatat accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     3601 gacgttgatg ccctggtaaa ggcggttcca ggtaaagccc agaatacggt cagtcaggcg
     3661 gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     3721 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     3781 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg gtcctacggc
     3841 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gcgcgatagt
     3901 tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac
     3961 aaaactgtcg cgaccgagcc acagtacagc gaaaaatttc tgtacgccgt taagcatacg
     4021 cagcggcggg ttcacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgacac
     4081 tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa
     4141 cagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat
     4201 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4261 gtcatgtgcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa
     4321 aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat
     4381 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4441 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4501 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4561 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     4621 caatggccaa taataccact ggattcaccc gaataatcaa agctgctggc tattcctgga
     4681 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     4741 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc gattacccgc gtgctgctta
     4801 tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg
     4861 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg
     4921 ccgcggtgct gattgcgatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc
     4981 attttggata acccttccag aattcgataa atctctggtt tattgtgcag tttatggttc
     5041 caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga
     5101 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5161 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5221 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgtaattga aattgtttcc
     5281 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc actggtaggt
     5341 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5401 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5461 aaagatatcg gcattatgga tggcgacttg ctggcagtgc ataaaactca ggatgtacgt
     5521 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     5581 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     5641 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gcgttattcg caacggcgac
     5701 tggctgtaac atatctctga gaccgcgatg ccgcttggcg tcgcggtttg tttttcatct
     5761 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actttggcat
     5821 ctcgccttac ccatgatttt ctccaatatc actgttccgt tgctgggact ggtcgatacg
     5881 gcggttattg gtcatcttga tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg
     5941 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6001 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaacctttg
     6061 ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacgccgat tatcgatctg
     6121 gcgctgcata ttgttggcgg tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa
     6181 atccgctggt taagcgcacc ggcgtcgctg gcgaatctgg tattacttgg ttggttactc
     6241 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6301 gtgctggatg tctggctggt gatgggactg catatgaacg tgcggggcgc ggcgctggcg
     6361 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6421 aaactacgcg gaatctccgg cgaaatgctg aaaacttcct ggcgaggaaa cttccgtcgc
     6481 ttgctggcac ttaaccgcga tatcatgctg cgctcgctgt tgttgcaact ctgtttcggc
     6541 gcgatcaccg tacttggcgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg
     6601 atgacgctac tcacctttac cgcctatgcg ctggacggtt ttgcctacgc ggttgaagca
     6661 cattccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca
     6721 gcatgccgcc agtcggggat cgtagcgtta ctgttttcgg tggtttattt gctggcaggg
     6781 gaacacatca ttgcgttact ggcgtcgtta acccagattc agcagctggc cgaccgctat
     6841 cttatctggc aggtgatttt gccgttggtc ggcgtctggt gttatctgct ggacggcatg
     6901 tttataggcg caacgcgtgc tgctgaaatg cgtaacagta tggcggtggc cgccgcaggt
     6961 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gttggcgtta
     7021 accgtctttc tggcgttgcg cgggctttct ctggcagcta tctggcggcg tcactggcgc
     7081 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc
     7141 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7201 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7261 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7321 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7381 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7441 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7501 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7561 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     7621 cgtatgcatg atgtcttcca cgccttcagc acattcttct ttcaccgcgc cgcagcgatc
     7681 gcaaataaac atagctgacg tatgggtggg ctggtcgaac agatggcaga gcacataact
     7741 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     7801 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca gatcataagc
     7861 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     7921 gcgcacatta cgctgcgcgc agattttgtc agcctgcgct aataactcct gcgttgtggt
     7981 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8041 gaaaaacacc gagaaccctt attgttgccg taatgttgct tttctgtttt gtaggtaaga
     8101 tgttgtgttg cctggcagta
//
LOCUS       DAZFTN010000170         8109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFTN010000170 DAZFTN010000000
VERSION     DAZFTN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1733
                     /gene="adeD"
                     /locus_tag="ACHKV2_004612"
     CDS             <1..1733
                     /gene="adeD"
                     /locus_tag="ACHKV2_004612"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO6942362.1"
                     /translation="SRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKG
                     RYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTT
                     VICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQML
                     AWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAG
                     IENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNP
                     WEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLL
                     SDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQPVSASDFALQFTP
                     GKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRLAPACGLLGGFGL
                     NEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAG
                     LMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEK
                     FAFTTLEVTE"
     gene            complement(1779..3170)
                     /gene="uhpT"
                     /locus_tag="ACHKV2_004613"
     CDS             complement(1779..3170)
                     /gene="uhpT"
                     /locus_tag="ACHKV2_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO6942363.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3308..4627)
                     /gene="uhpC"
                     /locus_tag="ACHKV2_004614"
     CDS             complement(3308..4627)
                     /gene="uhpC"
                     /locus_tag="ACHKV2_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6942364.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4637..6139)
                     /gene="uhpB"
                     /locus_tag="ACHKV2_004615"
     CDS             complement(4637..6139)
                     /gene="uhpB"
                     /locus_tag="ACHKV2_004615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO6942365.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6139..6729)
                     /gene="uhpA"
                     /locus_tag="ACHKV2_004616"
     CDS             complement(6139..6729)
                     /gene="uhpA"
                     /locus_tag="ACHKV2_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO6942366.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6891..7994)
                     /locus_tag="ACHKV2_004617"
     CDS             complement(6891..7994)
                     /locus_tag="ACHKV2_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942367.1"
                     /translation="MNSYIKIDQTGSNKFKITDLYREFNGETKCYDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDTQTERRMIELIRE
                     QKDLIPEKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKINSTISELL
                     SKKITDLTHPTESFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNY
                     HQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
BASE COUNT         2104 a         2219 c         1997 g         1789 t
ORIGIN      
        1 ttagccgggc ggaataccag gaattgttag ccgtttcccg tggcgacgct gttgccgatt
       61 atattattga taatgtctct attctcgacc tgattaatgg cggagaaatt tccggcccaa
      121 ttgtgattaa agggcgttac attgccggtg ttggcgcaga atacactgat gctccggctt
      181 tgcagcggat tgatgcccac ggcgcaacgg cggtgccagg gtttattgat gctcacctgc
      241 atattgaatc cagcatgatg acgccggtca cctttgaaac cgccaccctg cctcgcggcc
      301 tgacaactgt tatttgcgat cctcatgaaa tcgtcaacgt gatgggcgaa gccggtttcg
      361 cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag gtcagctctt
      421 gcgtacccgc tctggaaggc tgcgatgtta acggtgccag ttttaccctt gaacagatgc
      481 tcgcctggcg ggaccatccg caggttaccg gccttgcaga aatgatggac taccctggcg
      541 taattagcgg gcagaatgcg ctgctcgata aactggatgc atttcgccac ctgacgctgg
      601 acggtcactg cccgggtttg ggtggtaaag aacttaacgc ctatattgct gcgggtattg
      661 aaaactgcca cgaaagttat cagctggaag aaggacgccg gaaattacaa ctcggcatgt
      721 cgttgatgat ccgcgaaggg tccgctgccc gcaatctcaa cgcactggca ccgttgatca
      781 acgaatttaa cagcccgcaa tgcatgctct gtactgatga ccgtaacccg tgggagatcg
      841 cccatgaagg acacatcgat gccttaattc gccgcctgat cgaacaacac aatgtgccgc
      901 tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg ccactttggt ctgaatcacc
      961 tcggcttact ggcacctggt aagcaggctg atatcgtcct gttgagcgat gcgcgtaagg
     1021 tcacggtgca gcaggtactg gtgaaaggcg aaccgattga cgcgcaaacc ttacaggcgg
     1081 aagagtcggc gaaactggca caatccgctc cgccatatgg caacaccatt gcccgccagc
     1141 cagtttccgc cagcgacttt gccctgcaat ttacgcccgg aaaacgctat cgggtcattg
     1201 acgtcatcca taacgaattg attacgcact cccactccag cgtctacagc gaaaatggtt
     1261 ttgagcgcaa tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa cgtctggctc
     1321 cggcttgtgg tttgctcggc ggctttggtc tgaatgaagg cgcgctggct gcgacggtca
     1381 gccatgacag ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg gcgctagcgg
     1441 tcaatcaggt gattcaggat ggcggcgggt tgtgcgtggt acgtaacggc caggtccaaa
     1501 gccatctgcc gttacccatt gccgggctga tgagcaccga cacggcgcag tcgctggcgg
     1561 agcaaattga cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc gatgagccgt
     1621 ttattcagat ggcgtttctt tccctgccag tgatccccgc gctgaaacta accagccagg
     1681 ggctgtttga tggcgagaag tttgccttca ccacgctgga agtcactgaa taataaaaaa
     1741 agcccggcgt catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga
     1801 atttttttct cgcggcggat tttgcgttct tccatcaccg ccactatcgc catcaggcag
     1861 atacaaccaa tcgcggcgat atccagcgca gcgaaggtgc ctgcccagcc ggtaaggccg
     1921 aatactggcg tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc
     1981 aggtaagcga aggtgccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag
     2041 ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag
     2101 agagaagcca gatagatata ttcgttgctg gcatgttggt acacaccgag cgtggcgata
     2161 atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgctag gtcagagagc
     2221 cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc
     2281 gccaccgctt tagagagttt cagttcctgg aacgcgtaca cagttgacca ctggtcgata
     2341 ccaatacgca ccacatagag gaaaatgttg gcgaagcaca gcagccagat cactttgttt
     2401 ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctctttg
     2461 tcctcttcgc tgatctcctc gccgaacagc tcttcagctt tgccgaggcc ataagattcc
     2521 ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat aatcgacggg
     2581 aagataaaca tgccgatgac atggccatcg aacaggtaat ttgcgccgaa cagtgccaca
     2641 ccagctgcgc ctgcaccgcc aaggttgtga gaaatgttcc agaaaccgag gaacgtcccg
     2701 cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc cccggtactc
     2761 tggaaaaagc cacttaaggc gtagaaggca atcatcagga acaggctaac cgagccgctg
     2821 cccatactgg cgctaaagcc cagcatacaa atggcagaca ggatcagcat gaacggcagg
     2881 aattgtttgg tgtttttgcc gtcggcgtag taggaaacca gcgttttacc cacgccataa
     2941 gtgatggaga aacccaggcc gatcatcccc agctgcgtca tgctcaaccc gtaggtcgaa
     3001 atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt caggtagccg
     3061 ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg
     3121 agcggaaggt ccagggtcgg cttgcggacc tggtttaaga aagccagcat gggttactcc
     3181 tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga
     3241 tagcgtccag gcaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg
     3301 agatgcatca cgcttcgcgc ggtgtctggg cgttcaaaaa gggcagtaac agcagtgcgg
     3361 aaatcccggc ggcaatggcg ataaccacaa aaaatccgct ccagtgccag gtatcgagca
     3421 ctttcgccag cggccaacca gcaagcgacg cccccagata agcaaacaag ccgacaaacc
     3481 ccgtcgccgc ccctgccgcc tctttgtggg aacattctgc cgccgccatg ccgattaaca
     3541 tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc atcacgtagc
     3601 tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggca gcgaaaatca
     3661 aattcatcgg cccgcggttg ccgttaaaca gtttgtccga gccccaaccc gctaccagcg
     3721 caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg accagatcga
     3781 cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gatcgccgcc cgaaccacat
     3841 agaccagcac atagcaaaac gaaagcagcc agatatacgg attcaacaac acatacttgg
     3901 tgaggatctc tttacgcgtc agccctgccc cttcttgttg ttgagcaatc tccagcgcgt
     3961 cgtgtcgcca ttcaccgacc gctggtaaac ctaacgcctg cgggcgatcg cgcagccgcc
     4021 agcagagaaa aatccccacg acgatcgcca tacaaccagc aatcatcatc ccggcacgcc
     4081 agccgtaatg cagcgcagct gctgccatca caatgggaat gagcgcaccg ccaacgttat
     4141 gcgccgtgtt ccataatgcc caccaaccac cgcgttcggt acgagaatac caggccgtta
     4201 acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc acccagagca
     4261 cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttgatgatg cccgtggcga
     4321 taagccctat ccccataaaa taacgggcat ttgagcggtc gctgacaatg ccggagacaa
     4381 acttcgacac gccgtaggta atgtaaaaca gggtcgctaa caggccgata tcgctacggc
     4441 tgagcacgcc attagcaagg atttctggta cggcggcgtt aaaactttta cgcgtgaagt
     4501 aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag tagcgataac
     4561 gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg
     4621 gcaacatcat caaaccttag acatagcgtt gaggtagaga aacgctgaca cgcgtgccgt
     4681 gcagacagga aatggtcaag gtgccgccca gcgccgttac gcgctcgcgc attccggtga
     4741 ggccaaaacc ttgttgcccg gaacccggcg gtaaaccgct gccatcgtct tcaatcacca
     4801 gcatcaaccg ttcatcctgc agccagcctt gcagggtgac cgcgctggca tcagcatgtt
     4861 tcacaatgtt attcagccct tcctggcaga cacgaaacag cgtcacgcgc tggttttcgc
     4921 ttaacgctga ctcttcgatt cgccattcga gatggctgac aatgccgcgc ccttccagct
     4981 ccatttcccg catcagtgag cggatggcct gctctagggt gagatcatcc aactggcgcg
     5041 gacgtaaccg ccctaacaaa cggcgcaccg cgtcgtacac gcccagcgac agttgttcga
     5101 tgagctgccc gctctgcttc acactggcgt tatctgccgc cagtcgctgg acaatgcccg
     5161 cttgagtacg aatggcagtg atggtctggc cgatatcatc atgcagctca cgcgccacat
     5221 cacggcgcac gctctcttca gtttctaaca aacgttcagc gagatgctga ttgcgcgcca
     5281 gttccttttg cagcgactgg ttaagttcac gcaaccgctg gatgccagcg cccagtaaca
     5341 accctgtcag actttgcacc agcagcgaga gcaataaatc cacaggatga tcgcgccagg
     5401 tttgactggc gatcagcgcg atggcgttca tcaatgtcgc tatcagcgcc ccttgccagc
     5461 cgtagtgcca ggccagcgcg ataatcggca gcgccaggca gaatggcgta aagcgcgaca
     5521 gttcgtccgg caagcccaac tggagccaga gactgataac aaacagcagc aagtaccaga
     5581 ccagatgccg cccgcgccag ttgatcggtt gagaaactaa cgacggcccg agcggcagcc
     5641 aggtgttatt ggcgagataa tgccagaaca ccagacatat cggggccagc gtcaggccgc
     5701 cagttaaagt taacagcagc gcattccacg actctttgcc gtgccaaagc cagggcagcg
     5761 actgcaacaa cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg cgccagtcac
     5821 gttgatggcg atagcgcgag atcagcgcca cgggcagtaa cgtcagtaaa ctaccgatca
     5881 tcaataagga gaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc agcagccact
     5941 ccgctcccag cagcaccggc cagtatccgc gcgggcattg cagcattagc cctagccgca
     6001 gaccaaacgg aaataacagc accgccatat cagggcgctc aaccaggtgc aggctgatac
     6061 tccacaggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg gtaattaagc
     6121 gggagaacaa cgtcttcatc accagccatc aaacatacgg cgcgccagtt caacgtcgtt
     6181 actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt tcggtgacaa
     6241 gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt ccgccacctg
     6301 ccgttcacgt ttggttagtg ggtcctggcg accggatgcc agtttaatgg caatatccgg
     6361 cgtcagataa cagccgcctg tggcaaccgt atgcaccgca gcaatcagtt cgtcagggct
     6421 acaacgcttg gagagaaagc cacgcgcccc cgcgttaagc gcctgctcaa ccagcgccgg
     6481 actgtcatga acggagagca taattgtcgc catacctttc ggcagctggc ttagcagctc
     6541 cagaccggag atatcgggca tggagatatc gcaaatacac acctgcacac cgcgccccgg
     6601 cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacttgca aatcaggttc
     6661 cagccccagc aactgcgcaa agccggagcg gacgatgagg tgatcgtcta taagggcaac
     6721 ggtgatcatg attgggtcct ggcggataaa aaacgcgcat accttaacga taagcgcgat
     6781 gttgttcaag ttggatatgg ggtatgtatt ggcttaatga tgttgagctt atcgtcaaaa
     6841 cgcttacctg agaggtaagc gtttggtgcg tgttattgag aaaaagcgaa ttatttgtag
     6901 gttgcaatcg taatattgcc tgagaaattt ctccttagat tgcggatatc attatccctt
     6961 gcccaataat catctttgtg tctataactc tcatcggaat gtacataggg agaatcatct
     7021 tcagcgtcgt tatggataat attttcgcct ttagtggtat agatataatt agcaacatca
     7081 aaggtgtcat tcgcgttatt tacgactgcg cctaagacgc aaagggtttg agtgaatccg
     7141 tattctgaga aatcgacatg ctgatggtaa tttctaccat tcaaagcaac aaaactcgga
     7201 caaacatcta ttgtatcgcc aacagacgta gcggcgcgac gcacatcaag tgcagcgtta
     7261 tcgttagcaa catgaaatgc ttgtgaacag gcataaaaca aatttctaat cccggtacga
     7321 ctggataaac aagaaataat ttttgctatt ttttctggct ttataccatc ctgtactaaa
     7381 gaatctctca ccagatcaca ttcaccaggt gtgaaaacaa ctttcttgaa tatttcctca
     7441 taaacatcat aagaaaaata ttcagagaga gtgaaagatt cggtgggatg tgttaaatct
     7501 gtaatttttt tgctaagtaa ttcacttata gtgctgttta ttttttcacc gcaacataaa
     7561 acaaatgaac ttccctccag aacttgcctt tctgcatcta caaggagtga gctaaattca
     7621 tcctcatgaa gcttaagttt ttttatacct gattgatgta aatatttttc tggaattaaa
     7681 tctttttgtt cccttataag ctctatcatt cggcgttcgg tttgcgtatc aaaagagtat
     7741 ttcatgttat agttattttt agcaacttcc agcttaagag catttgcagc aactgttaat
     7801 tctgagattt gatctttttc aaattgatcg tgatctgata ataaccttat aaactctttt
     7861 acatttcgac cactaaaatc catatcccta cactttttat cataacactt tgtttcgcca
     7921 ttgaactcac gatacagatc agtgatttta aatttattac ttccggtctg atcaattttt
     7981 atatagctat tcatgcctgt aacggcaatc tatgtcttat aattcgcatt ctccgattca
     8041 ggcgtaacat gaaaataagg aaccaattta tcactaatat catgccgata gatatgaaat
     8101 gaatctaaa
//
LOCUS       DAZFTN010000171         8106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFTN010000171 DAZFTN010000000
VERSION     DAZFTN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..2129
                     /gene="iha"
                     /locus_tag="ACHKV2_004618"
     CDS             39..2129
                     /gene="iha"
                     /locus_tag="ACHKV2_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO6942368.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2347..2497)
                     /locus_tag="ACHKV2_004619"
                     /pseudo
     CDS             complement(2347..2497)
                     /locus_tag="ACHKV2_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2709..3029)
                     /locus_tag="ACHKV2_004620"
                     /pseudo
     CDS             complement(2709..3029)
                     /locus_tag="ACHKV2_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(3452..4027)
                     /locus_tag="ACHKV2_004621"
     CDS             complement(3452..4027)
                     /locus_tag="ACHKV2_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO6942369.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(4096..4674)
                     /gene="terD"
                     /locus_tag="ACHKV2_004622"
     CDS             complement(4096..4674)
                     /gene="terD"
                     /locus_tag="ACHKV2_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO6942370.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4723..5763)
                     /gene="terC"
                     /locus_tag="ACHKV2_004623"
     CDS             complement(4723..5763)
                     /gene="terC"
                     /locus_tag="ACHKV2_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO6942371.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(5786..6241)
                     /locus_tag="ACHKV2_004624"
     CDS             complement(5786..6241)
                     /locus_tag="ACHKV2_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6942372.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(6264..7421)
                     /locus_tag="ACHKV2_004625"
     CDS             complement(6264..7421)
                     /locus_tag="ACHKV2_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO6942373.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(7421..8002)
                     /locus_tag="ACHKV2_004626"
     CDS             complement(7421..8002)
                     /locus_tag="ACHKV2_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO6942374.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2061 a         2058 c         2037 g         1950 t
ORIGIN      
        1 tattgtcttg ccggttaaca tgatcggaga ttagtaatat gcgaataacc actctggctt
       61 ccgtagtcat tccctgtctc ggattttcag ccagcagcat agctgctgca gaggatgtga
      121 tgattgtctc ggcatccggc tatgagaaaa agctgactaa cgcagccgcc agtgtttctg
      181 tgattagcca ggaggaattg cagtccagcc agtaccacga tctggcggag gctctgagat
      241 cagtagaggg tgtggatgtt gaaagtggta cgggtaaaac cggagggctg gaaatcagca
      301 tccgaggaat gccagccagt tacacgctga tactgattga tggtgttcgt cagggcggaa
      361 gcagtgacgt gactcccaac ggtttttctg ccatgaatac cgggttcatg ccccctctgg
      421 ccgccattga gcgtattgag gttatcaggg ggccgatgtc cacactgtat ggctctgatg
      481 cgatgggcgg tgtggtgaat atcattacca gaaagaatgc agacaaatgg ctctcttccg
      541 tcaatgcagg gctgaatctg caggaaagca acaaatgggg taacagcagc cagtttaatt
      601 tctggagcag tggtcccctt gtggatgatt ctgtcagcct gcaggtacgc ggtagcacac
      661 aacagcgtca gggttcatcg gtcacatcac tgagcgatac agcagccacg cgtattcctt
      721 atcccacgga gtcacagaat tataatcttg gtgcacgtct tgactggaag gcgtcggagc
      781 aggatgtgct ctggtttgat atggatacca cccggcagcg ttatgataac cgggatgggc
      841 aactggggag tctgacgggg ggatatgacc ggaccctgcg ctatgagcga aacaaaattt
      901 cagctggcta tgatcatact ttcaccttcg gaacatggaa atcgtatctg aactggaacg
      961 agacagaaaa taaaggtcgt gagcttgtac gcagtgtact gaagcgcgac aaatgggggc
     1021 ttgccggtca gccgcgggag cttaaggaat cgaaccttat cctgaattca ttactgctta
     1081 cccctctggg agaatctcat ctggttacgg tggggggcga gtttcagagc tcgtccatga
     1141 aagacggagt tgtccttgcc agcacaggtg aaactttccg gcagaaaagc tggtcggtat
     1201 ttgctgagga tgagtggcat ctcacggatg cacttgcgct gactgcgggc agccgctatg
     1261 aacatcatga gcaattcggg ggacacttca gtccgcgtgc atatctggtc tgggatgtgg
     1321 cagatgcctg gacgctgaaa ggcggtgtga ccacgggata taaggcaccc agaatggggc
     1381 agctacataa agggattagt ggtgtgtccg ggcagggaaa aacaaatcta cttggtaacc
     1441 ccgacctgaa gccggaagag agcgtcagtt atgaggctgg ggtgtattac gataaccccg
     1501 ccggtctgaa tgccaatgtc acaggtttta tgactgactt ctccaacaag attgtctctt
     1561 attccataaa tgataacacc aatagctatg taaacagcgg aaaggcccgg ttgcacggtg
     1621 tggaatttgc cggcacattg ccgctgtggt cagaggatgt cacgctgtca ctgaattaca
     1681 cctggacccg aagtgaacaa cgtgatggtg ataacaaagg tgcgccgctg agttataccc
     1741 ctgaacacat ggtgaatgcg aaactgaact ggcagatcac cgaagaggtg gcatcatggc
     1801 tgggtgcccg ttatcgcggg aaaacaccac gtttcaccca gaattattcg tcactgagcg
     1861 ctgtacagaa gaaagtgtat gatgagaaag gagaatacct gaaagcctgg acggtggtgg
     1921 atgcaggtct gtcgtggaag atgacggatg ccctgacgct gaatgctgcg gtgaataacc
     1981 tgctcaacaa ggattacagt gacgtgagcc tgtacagtgc cggtaagagt acgctgtatg
     2041 ccggtgatta cttccagacg ggatcatcaa caacaggata tgtgatacct gagcgaaatt
     2101 actggatgtc gctgaactat cagttctgat aataacaaaa cgctatcact gacggtagaa
     2161 tacgttgcca ctgcaactcc tggcggaaca gtggcaacgt attaggttaa gtgcatttcc
     2221 gatccgctaa tgagatttcg ttaccaacaa ctaatatcgt cacaggaaat gcacggatta
     2281 tttttaactt atcatttaca tacttgtcca gagtgtaagc gcaccgtgaa ggacgtgggg
     2341 taaaaattag tttacagaga gagtgacgtt ccagggcaac aactctttca tgcggttggc
     2401 aggccaggtg ttgattacac tgatcacgtg gcgtagccac gtttccggct cgattccgtt
     2461 aagttttgca gctaccgatc aggctgtaca tcactgccgc actatcgctc gtcatctcaa
     2521 agtcctgtct cgtcagcagg aaggtatcat tctctcccgc catttttcca ggggcccggt
     2581 cagataagtc cctttgtcta tcgctgactc ctgactcata acccggttag cagaatgcag
     2641 ggtcaccacc tcgccacgac caaatccaaa taagtcaatt gcaccttctc aatcgccatt
     2701 ttgtcagtaa gcgtacagcc tgaactgatg gtatcttcac catcaatgac aacggtgatc
     2761 gcaattttac tgacgttcgc cggaacacga tccagtgcta tctcaatgct ggcctgctgc
     2821 gaaccggtaa cgagcctgac tgccccctca ggagaagaca aattattata aaagataaag
     2881 tcagaatcgc cactgacctt tccctgagca ttaagcatga acaggcaggt atcgggttcg
     2941 cctttaaagc cggatttggc agggtactga agattcagcc tgatcgtaga ttgctgaagg
     3001 ggtatgtttt gtccggattg taaattcata ttaactctcc tgatttttga ttattattaa
     3061 tgcgcagcgt ttatatatgt tccctaggct tagttctgga cgctggatat tcggtgaggc
     3121 gtaaatatgg tatgacacca tttttcataa cgctgaagtt tctatacctg ttgaatttga
     3181 attttcattg accgggtatc ttattttcca gggcccgttc cttcataagt cgcaaaagta
     3241 acatatatcc gaagggcatg ctgttgatat cagacacgga atactggctt taaccagcaa
     3301 ccacagataa acccggggcc tgcataaaag gttcatgcca gaattaacat agctctgctt
     3361 tctgcccccc ccctttccca tattcacccg ttgatagcgg atcgataccc aaaaaaaacc
     3421 ccggcattgc cggggtcaga ctcagcgtct tttagacatt gacgccgtgc tgggaagcaa
     3481 gcgctgacag accaccggca aaaccctggc caacagcttt aaacttccac tcagtaccat
     3541 ggcgatacag ttcaccgaag accattgcgg tttcggttga ggcgtcttca gacagatcga
     3601 aacgggcaat ttccgtcccg ttgtcgttgt tgtaaacgcg catgaagctg ttgctcacca
     3661 tgccgaagtt ttgtttacgc gcttctgcat catagatggt aacggcaaat accagttttt
     3721 tgatgtctgc tgagactttg gtcagatcga ttttgacctg ctcatcgtcg ccgtcgcctt
     3781 caccggtacg gttgtcgccc tggtgctcta ctgcgccatc agggctggtt ttattattga
     3841 agaaaatgaa atgggcatct gacagtactt taccgtcttc acctactgcg aatacggaag
     3901 cgtccagatc aaaaccctga ccatcggtta cacgggcatc ccagcccagg ccaaccatag
     3961 cgacattcat ggttggtgct tctttggtca gagatacgtt gccgcctttt acgagagaaa
     4021 ctgccatttt tagctcctgc aaacagttga atgaggctga attaacagcc ccagatatga
     4081 aaagttactt ttcgatcagg acgcgttaat gccgtactga gcacatacag atgccagacc
     4141 accagcataa ccctggccta ctgcgcggaa tttccactca ccattgtggc gatacagctc
     4201 gccgaacagc atggcagtct cagtggacgc atcttcggtc agatcgtagc gagcgacttc
     4261 agtctggtta tcgtcattaa ccagacgaat aaacgcaccg gatacctgac caaagctctg
     4321 gcgacgagcc tgagcatcgt ggatggtcac aacgaagatg atcttgtcaa cttcagacgg
     4381 gacggcgtcc agtttaattt tcagcgattc atcatcacca tcgccctcac cggtgcggtt
     4441 atcgccggtg tgcgttacgg aaccgtcgga tgacgtcagg ttgttataga agatgaaatc
     4501 tgaatcgccg cgcactttgc cgtttgaggc cagcaggaat gctgaagcat ccaggtcaaa
     4561 gtcctgaccg tctgttgaac gcgcatccca gccaaggccc accaggacat ttttcattga
     4621 cggagctgct ttactcaggg agacgttccc gcctttggaa agagaaacac tcataaaata
     4681 acctcttcga ttagtaattg ttcaggttaa cacttaaggg gattaactcc ccttttcctc
     4741 agattcaggt ttgcccggga acatgacgct tgcgagaatg cccagcgcca gtacacccag
     4801 tacaacatac aggctggttg ttgccgcgat gctgtaacca tgatgccaga tgtgatcggt
     4861 cgcattcagg ccgagttttg ccgcgatgaa gaacagcagc acgataacgg ccttctccag
     4921 atgaaccagg tactgtttca gtgcctcaag gacaaaatac agagtacgca gacccaggat
     4981 agcaaacatc atggcactat agacgataag cggttcacga ctgacggcaa tgattgccgg
     5041 taccgagtca aacgcgaaca ttacgtccga gagttcaacc acagccacac acaggaacag
     5101 cggggtcgca tacagcgccg cttttttacc acggccaatg gtgacatcgc tgttttctgg
     5161 tttcgccagt tcagcatcca cttccttctg gttaagtagg aaggcatgcc ctctgagctt
     5221 cggccagata gggaagaagc gtttaaccat acggtaagcc agatgctggg agtaatcctc
     5281 aatttcatca tcgtcatcac cgcttttaag catcatgacc gccgtccagg caacgataat
     5341 agcgaagaca acttcaacgt acggccccag actcagcagg ctcgtaccga tggcgacaaa
     5401 gatgcccctg aagacgatgg caccaatgat cccccagtag agaacacggt ggcgataacg
     5461 atccggaacg gcgaaccagg agaagattgc catcatgacg aacaggttat cgaccgacag
     5521 cactttctcc agcgcataac ccgtgacaaa cagactggca acctcagcac cgtggtggat
     5581 atagaggaaa ccggcaaatg ccatcgcaac cacaacccag aatacggacc agagcgcagc
     5641 actcttcagc gagattggct tgtcatcacg gtgcataaac aggtcaataa agatggcacc
     5701 gactgaaagc gcaataaaaa caatgaccgt ttcagtcggg aagccgatgt gtgtggaaac
     5761 catattttac ccttagggat gtggatcaga ggccaaattc agccgggtcg aagcccaacg
     5821 cgatagcgat ctcgcgggag gccagtttct cgtcctgatc gaagttaccg tcacttttcg
     5881 caacggcaat accaacacgt aaggccagct gagcggcctc aggctgatct ttcagcgcca
     5941 ggatgtattt catggtttcg cccttgccaa tttcaacatc gaaatcgaag cttgaaacca
     6001 gtttattgaa gaactcaatc acctcattgg tatcgaagac cttcagctct tctgaagagc
     6061 gcagaaagcc catcattttc tgcttttctt ccgaacttac gccgtcactc gatacggcaa
     6121 tacgggcaca tacagcaacg gtgccctgca tgaatttttt gtttttgaaa cggccaacct
     6181 ggcgggtcag ttcgtcacgg cctgagttaa tggcaccttt aactttgtcg aaaaagctca
     6241 tacctgtttc ctttgttgct gtgttattta gaaccggcgc tccagcgaaa tccccagcca
     6301 aatgcccggt ccatttcgtc ctggcctttg aagtactggt taattcgctc gactttaatt
     6361 gcgccgtttt cgtttacgag tctggcaatg gcgcacaatg tgcgacggtt ctcaccttcg
     6421 gtcaggcggg tctcaatggg cggttgatcc ggcacatgaa tggtgactac accatcagtc
     6481 ttgtcccagc tgggaacacc ttcataaata aaggcgtaaa tcagcacttc gcggatatgc
     6541 ttccattcac gtccattgat atgcagccac tcgccgtctg atacatcacc cgtccggtca
     6601 tcgcccttga gctgaacata cggttcatcg cgataatcac cgaatgcatt accgagcgcc
     6661 tgaatgaccg atttataccc gtcctgaagc tcaacaaagg cccccagatc caggtcgatt
     6721 cctttggagc cgaacatacc tgcaaagcct gatttgctac tgccccggtg ccagttcagg
     6781 ttgatccgga tttcaccgaa gttatcccgt ttggtcaggc tgattgcagg cttctctttt
     6841 gtcagcgata ccttgctcag gctgattttt gtctcaactg gcggaggggt tacaaccgga
     6901 ggagttgggg ctgcgggagc cggttcgtcg gcaatattta cgccgaaatg ctctgccagc
     6961 ggtttaagtc caccgttgaa cccctgcgca acgaagcgga atttccagtc attattacga
     7021 cggtaaaatt cccccagaat taaggctgct tcctgacggc cgctcagttc gacactgccg
     7081 ctaaccaggc cggtagcccc ttgctccacg tcgatggaaa gattacgaag accggacacc
     7141 gtctgcccac cgtcacaggt cacggtgaac gcaattttct ggacatcagg tttcagccgg
     7201 ttgagcgcga cagtaaaggt tgagtactgg ccttcactga ccaggctgac ggtaccatca
     7261 tcgttacgtg gctgaccata aaacaccatg tccgcatcac cctgcacctt cccgtcggca
     7321 tacaggcgaa acgccgaagc atccacctgg ccgcccgagg tgatccggac ccgcaactcc
     7381 tgggctggaa cgggggcatt gccccccggc gtcaggttca ttagcgaata accgctgaaa
     7441 cgatatcgga gtgcatatcg tcaattgtgc ggccgcgaca ggcatggcca agggcggtaa
     7501 agtcccagtt gccgttatta cggcgcagcg aagaaataac gatcccggta tgtgagccct
     7561 gctcagtcag cttgtagcga gccagctctt tgccggtttg atcgacaacg cggcagaaag
     7621 cgttttcgac gtcattgaac gactgaccac ggaagctgtt taccgtaaac gccaggtatt
     7681 caacgtttgc aggcaggcgg gagaggttga ccttaatcac ttcatcgtca ccgtcaccct
     7741 caccggtcag gttatcgcca ctgtgcacaa cagcgccgca ggtggatttc agtttgcgga
     7801 accagatggt gtcaattgtt ttacctgtgc tgtccattaa tacacagcca gcatcgagat
     7861 cgattgagtc gttgcctcca aacaatcctc caagaagacc ttttttctta accggatccc
     7921 aaccgagacc gaaatgaagc tggcttaatg cagatgattc tttgctgagt gataccgtct
     7981 ggttcttaac taatgaaacc atgttttatt ccttaaaatg gtaatgaccg agacaatcaa
     8041 aggtatattt agtaactgac gaagaaaatc atatcatgat gaagttctct gacaagggaa
     8101 attttt
//
LOCUS       DAZFTN010000172         8094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFTN010000172 DAZFTN010000000
VERSION     DAZFTN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            172..756
                     /locus_tag="ACHKV2_004627"
     CDS             172..756
                     /locus_tag="ACHKV2_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6942375.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(781..873)
                     /locus_tag="ACHKV2_004628"
                     /pseudo
     CDS             complement(781..873)
                     /locus_tag="ACHKV2_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            874..1620
                     /locus_tag="ACHKV2_004629"
                     /pseudo
     CDS             874..1620
                     /locus_tag="ACHKV2_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1992..2258)
                     /gene="minE"
                     /locus_tag="ACHKV2_004630"
     CDS             complement(1992..2258)
                     /gene="minE"
                     /locus_tag="ACHKV2_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO6942376.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2262..3074)
                     /gene="minD"
                     /locus_tag="ACHKV2_004631"
     CDS             complement(2262..3074)
                     /gene="minD"
                     /locus_tag="ACHKV2_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO6942377.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(3098..3793)
                     /gene="minC"
                     /locus_tag="ACHKV2_004632"
     CDS             complement(3098..3793)
                     /gene="minC"
                     /locus_tag="ACHKV2_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO6942378.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4313..4681
                     /gene="ycgJ"
                     /locus_tag="ACHKV2_004633"
     CDS             4313..4681
                     /gene="ycgJ"
                     /locus_tag="ACHKV2_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO6942379.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4784..5185)
                     /gene="pliG"
                     /locus_tag="ACHKV2_004634"
     CDS             complement(4784..5185)
                     /gene="pliG"
                     /locus_tag="ACHKV2_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO6942380.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5256..5414
                     /locus_tag="ACHKV2_004635"
     CDS             5256..5414
                     /locus_tag="ACHKV2_004635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942381.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            5426..5719
                     /locus_tag="ACHKV2_004636"
     CDS             5426..5719
                     /locus_tag="ACHKV2_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO6942382.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5791..6450
                     /gene="ycgM"
                     /locus_tag="ACHKV2_004637"
     CDS             5791..6450
                     /gene="ycgM"
                     /locus_tag="ACHKV2_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6942383.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6542..6988
                     /gene="ycgN"
                     /locus_tag="ACHKV2_004638"
     CDS             6542..6988
                     /gene="ycgN"
                     /locus_tag="ACHKV2_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO6942384.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7195..>8094)
                     /gene="hlyE"
                     /locus_tag="ACHKV2_004639"
     CDS             complement(7195..>8094)
                     /gene="hlyE"
                     /locus_tag="ACHKV2_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO6942385.1"
                     /translation="VADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELS
                     RFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNE
                     KKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSS
                     YFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFF
                     TTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAANK
                     MINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         2114 a         1774 c         1935 g         2271 t
ORIGIN      
        1 tgtgcgtcac tgtatccagg actgttatgt gccaaataag gaagttgaag ttaccagctc
       61 ttactctaaa gctataacgt atagaaaaaa tgcatttgag acgtgtaaaa tcatttattt
      121 aatcgcaatt tatgtaatgg cataatcagg tcggtgaaag aggggagaag aatggataaa
      181 agagctaaaa atcagatagt cgatagcgat attgcacgtt tgttactcaa gctcagaaag
      241 tctcgaaact tgactgttac agagcttgcc caacgatctg gtgtcagtca ggcgatgatc
      301 agcaaagttg aacgaggcac ttcaagcccc agtgcaacaa tacttagtcg gttagctaat
      361 gcactgaata ttacgctatc aaagcttttt gcagagcttg agatgcaaca gaattcactt
      421 gtacttttgg ctgaccagca acaacattgg attgatgaag aaacaggcat tacccgctgg
      481 tctttgtccc cagcgggtgc atgtccggaa ctaataaaag ttgaaatccc ccctatgggg
      541 caattgacta tccctgcctc tgcaaatgag catctttctg gacaaactct atggatgtta
      601 agcggcagcc ttgattttcg agtcaatcat cagactcatc atttacaagc cggtgattgt
      661 cttgctctta cctttgccag cgagtaccaa atgagaaacc cagatagcgg ccagagttgt
      721 tcttacattg tcgctttcag ccaaaaaaat ccatgatgta attctcctaa aattatcatg
      781 tcacattgcg ttataaagat gtaatagagg gtagggtctg ccttgattgt cccgctcgga
      841 acgtcctgaa agtttaaagc ccatatgttg ataaatgcgg gtgccgggtt tgagcagacg
      901 ctgaccggaa tgacagtggg gatcgacagc cgtaatgata ttcctgaggg gattgcgacg
      961 ctgggcgctt ttatgggtta ttcccattca catatcggtt ttgatcgtgg aggacatggc
     1021 agtgtggaca gttattctct gggcggctat gccagttggg aacatgaaag tggtttctat
     1081 ctggacggtg tcgtgaagct gaaccgtttt gaaagtaacg tagccggtaa aatgagcagc
     1141 ggtggagccg ccaatggcag ttaccatagc aacgggctgg gcggtcacat tgaaaccggg
     1201 atgcgattta ccgatggtaa ctggaacctg acgccgtatg cctcgttaac ggggttcacc
     1261 gctgataacc ccgaatatca tttatccaat ggcatggaat cgaaatcagt cgatacccgc
     1321 agtatatatc gtgaactggg tgcaacgctg agttacaaca tgcgtctggg gaacggtatg
     1381 gaagttgagc cgtggctgaa ggcggctgtg cgcaaagaat ttgtcgatga taaccgggtg
     1441 aaagtgaata gtgacggtaa tttcgtcaat gatttgtcgg gcagacgtgg aatataccag
     1501 gcaggtatta aagcctcatt cagcagtacg ttaagcgggc atcttggggt ggggtatagc
     1561 aacggtgctg gtatggaatc cccgtggaac gcggtggctg gtgtgaactg gtcgttctga
     1621 ccatcaacga ttaaactgcg cttcggcgca gttttcgttt acaggatgtt aaaagagaaa
     1681 attctggggt aaaaaaagcc cgccagttac ggcgggaaac ctcatcctat gggagaacaa
     1741 tgaataatga aattgcgggg ttatcatctc ccagtatatc aacactaaca ataaggttat
     1801 ttactcaacc aggcataaat attttgtttt gtgcgtggga acagccttaa ggtgtaaagg
     1861 gggaggtgga aatagcaatg aggagtatca gcaagaatac tcgccgcttt accacaacgt
     1921 ggatgagagg gatgaaaaac tcaaggcaga gataactctg ccttgaagat aaatgcgctt
     1981 ttacagcggg attatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata
     2041 gaaatatcgc catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtacg
     2101 tatttacaaa tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca
     2161 tcgctgcgac ggcgttcagc aacaataatc tgcagccgtt cttttgcaat gttggctgta
     2221 tttttcttcc gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt
     2281 ttgaggaagc ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga
     2341 cgttctacgg tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt
     2401 tcaccctggt tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg
     2461 atgcgcagga tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct
     2521 gggttatagc gcgttaacag caggtgctct ttaataggct cttcgccatt ttctgcgcgg
     2581 cgtgattttg acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc
     2641 gggttggtgg taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca
     2701 atccctgccg gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga
     2761 actttagcga ccccttcacg ggtgagggca tctttatcgc gtgtttgcga tgccggcaga
     2821 atatagagat tttcagtacg cttatcttta attaacgcct gatttagcgt tgcatcgccc
     2881 tgaatgacgt tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga
     2941 ttacgcaggc cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca
     3001 gtggcgatgg ccgcgctgga ggttgtctta ccaacacccc ctttgcccga agtaacaaca
     3061 ataatgcgtg ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca
     3121 aagcgttttc gactaactgc agtcgcgccg ctttgccata aaattctgct gggatttgat
     3181 cactcagcca gtattcacct gcgatggaca ccaattccgc catcaggttc gtacaaaata
     3241 tttgcgtttc ccggtcacca cttgcccctg ccagcgcacg accgcgcatc atgccataga
     3301 catgaatgtt cccatcggca atcaattcgg ccccagcgct aacgtggctt gtaacaatca
     3361 gatcacattg tggagcataa atacgctgac cggaacgcac cggggtatct attaaacgcg
     3421 tttttgtgac cggcgttgta ttttgcgctg gagcctgcgg tgcgggagct ggacgtggcg
     3481 ctttttcctt tccttccgtc aggataggca gccccatttt ttcaatttcg gctttaagtt
     3541 gcgcatcttt gcagccactt acgccaataa cccgcaaacc ggttgccgaa accgccttat
     3601 gcatcgctga ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat
     3661 gttttaaaaa tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag
     3721 gttctgcctc atgcagatga accacagata aagtgaagct actgccttta agctcgattg
     3781 gcgtgtttga catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat
     3841 gttccgaaga ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca
     3901 tttattcaga gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata
     3961 cccttgtcaa tcaacccatt gccgtcgtac ttttgattct tcttatttac gcttcctttt
     4021 tccgcaccct aatcgtgaca gcatctccag acctgtttaa atatccagta ttgaggcaag
     4081 tgtatttttt caccacgcca ggctttctta actttccatt ttttgcagca tcattaaaga
     4141 acagataccc gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag
     4201 acgtctgata cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga
     4261 ttgatgatga aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat
     4321 gatgcgtatt ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc
     4381 tggacatgac gctggcggac ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat
     4441 ttgtgtgaac tcagatggca tttccccaga gttaacaagg aaatatctca gcgaaaaagc
     4501 cgctgaaaac ttacaatcat tacaaggcta cgatcccagc gaatttacat tcgctaacgg
     4561 tgtattttgc gatgttaaag aaaaattatg ccgtgatgat cgttattttg gtgtagatgg
     4621 taagcggagt ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg
     4681 atgtcttgtt tacattatgt tctattcgtc attaacctct tgagcgacag agaggagtgg
     4741 ggcgtcaacg tttatgacgg ggagactccc cgtcggttga catttactta atttgaatat
     4801 cgacgttata ttttttagtt ttgtttttac gggcgtcgtt tcgggtttgc agcactcgca
     4861 gctcatattt gccagaagca gggagtgagt attgtccgtg actatcgagt tcaggggaat
     4921 atctggataa atcaacagaa tcatcgattc ctggcccgaa gaggtaagta tcagcgcctt
     4981 cattagagat actcacatgc actttctgac cttttttggc atagaaggta tatgtatcgt
     5041 aatcgtatcc ctttatttcg ccggagtact gagcgctgct atggcctttt ctgaactcaa
     5101 cattgacatt cttacccgca gcaaagctcg tggaggttag cagtgcaagc agcaatacag
     5161 ccttactgat gctcttgatt ttcattggta gtcttctgtt cagaatgagt acgatgaact
     5221 gttataatat aacaatccct tacgggagaa ttccagtggc tttcctgcgt tcggtaaaat
     5281 cccgtcgcgg catttttgcc ggggggatta cttataaagg ggcatggact caacaaaccg
     5341 tagaaattta cagctggctg gcttgtggcg cgggtttcat tgatgttagc ttatgccaaa
     5401 acctggcatc ttaaagagta aaagtatgtt ttgtgtgatt tatcgaagca gcaagcgtga
     5461 ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg aggaactgat
     5521 gaaaggtttt ggtcagcctc agttagcgat gattctgccg ctggatgggc ggaagaaact
     5581 ggtcaatgcc gatattgaaa aggttaaaca ggcattaacc gagcaaggtt actatttgca
     5641 gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg ggcagaaaac
     5701 agacgacact aacaaataac cgatatccgg cggtggcatt atctttgtcg gcgcgggttt
     5761 tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg gcaaggtgcg
     5821 ctgctggatt atccggtgag taaagtagtt tgtgttggca gtaactatgc caaacatatt
     5881 aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc agaaacggca
     5941 ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt tcatcatgaa
     6001 gtcgaactag cggtgttgat tggcgcgacg ctgcgtcagg ctacggaaga gcatgtccgc
     6061 aaagccattg ccggttacgg cgtggcgctc gatctgacgt tgcgtgatgt tcagggaaaa
     6121 atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc ttgtccgctt
     6181 tccgggttta ttcccgcggc ggaattcacc ggcgatccgc aaaatacaac gctgggcctg
     6241 agcgtaaacg gcgaacaacg ccagcaaggt acgactgcgg acatgatcca taaaatcgtt
     6301 ccgctgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt tgtgctgaca
     6361 ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt cactttcgat
     6421 gggcattctt tgacaactcg cgttttgtaa tactttttgc cgcctgaaag cggcggcaaa
     6481 acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg cggaacacct
     6541 gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg atgcggaatg
     6601 ggagtcgttg tgtgatggtt gcggtcagtg ttgcctgcat aaactgatgg atgaagacac
     6661 cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa cctgtcaatg
     6721 tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa cccgtgaaaa
     6781 tctgccaaca ttcgaatggc taccaatgac ctgcgcttat cgtttgctgg cggaaggtaa
     6841 agatttacct gcgtggcatc cgctgcttac tggttcgaaa gcggcaatgc atggtgaacg
     6901 tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc aggatcatat
     6961 cttaaataaa cctgactggg cgcagtgata aatttaatag atgttgatgg gggctttctt
     7021 aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct ctgtcataat
     7081 gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa aacgtctgta
     7141 tttttaaatg tgataaactt tataccttga gtacatggag agaataattg ctgatcagac
     7201 ttcaggtacc tcaaagagtg tcttcttacc gtgtcttttc tgatactcat tacaggtgtt
     7261 aatcatttta ttggccgctt cttttagcaa agaaagcatt aaatcatcat aatcaacata
     7321 gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt cggtggttaa
     7381 tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag acagggtggt
     7441 aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt tcccttcaac
     7501 tacgcccgca gcaatagaat aggaaatgat taaaccaaat ggaccggcga cgacaccggc
     7561 tgcggcaccg gcatacgctt ccttcctgat tttatctacc tgtgactgga aatagctgct
     7621 tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc cggaagcgtt
     7681 gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct tcgtgatgcc
     7741 gtcatccagt accttaatga gaatgtcttt ctgggcggat gctttcttct cattatactc
     7801 atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc attcatacac
     7861 tgtttgggtt gcttcaaaat acttatcctg gctatccatt agtaaggttt taatattgcc
     7921 aactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact cttttatggt
     7981 ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa gatccaatgc
     8041 tccatctgcg gtttcgattg cgtttttaac tacctctacc gttttatctg caac
//
LOCUS       DAZFTN010000173         7972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFTN010000173 DAZFTN010000000
VERSION     DAZFTN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..853
                     /locus_tag="ACHKV2_004640"
                     /pseudo
     CDS             117..853
                     /locus_tag="ACHKV2_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1032..1328
                     /locus_tag="ACHKV2_004641"
     CDS             1032..1328
                     /locus_tag="ACHKV2_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942386.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1636..1752
                     /locus_tag="ACHKV2_004642"
     CDS             1636..1752
                     /locus_tag="ACHKV2_004642"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942387.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1971..3356
                     /locus_tag="ACHKV2_004643"
     CDS             1971..3356
                     /locus_tag="ACHKV2_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6942388.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
     gene            4177..4740
                     /locus_tag="ACHKV2_004644"
     CDS             4177..4740
                     /locus_tag="ACHKV2_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6942389.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            4895..7255
                     /locus_tag="ACHKV2_004645"
     CDS             4895..7255
                     /locus_tag="ACHKV2_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO6942390.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7417..7593)
                     /locus_tag="ACHKV2_004646"
     CDS             complement(7417..7593)
                     /locus_tag="ACHKV2_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942391.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(7731..7972)
                     /locus_tag="ACHKV2_004647"
                     /pseudo
     CDS             complement(7731..7972)
                     /locus_tag="ACHKV2_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2218 a         1581 c         1902 g         2271 t
ORIGIN      
        1 atcaatattt tctgaacagg gtaatagtac acgtattaac agattatgtc agaaggcccc
       61 ggtgatgcct tctgacgata gattaatgtt gtattctatt ggtatctgga gaccatatgg
      121 caacagttac agtatattct ccccgctgta attcctataa agtgtaccgt tatgatcaca
      181 gcacctcaga tcatgagcgt ttccagtgct aatcctgcaa acgtgtattt caactgaatt
      241 acatctacga aacccgaaaa ccaggcatta aagagcaaat tgttgaaatg gttcataacg
      301 gagccggggt tcgcgatagc tccagaacgc tgaaggtgga tatcaacacc gtcattctca
      361 ctttaaaaaa cgctcaccac gtaaagtaac catatctcca gccgccagtg tcgatgtggc
      421 acttatctgt gaacttgatg aacaatggag ttttgtcgaa aacaaagctc gtcagcagtg
      481 gcactggtac gcgtataaga ccaaagctga cggtgtgctg gcttacactt ttggtcctcg
      541 cactgatgaa acatgccgtg agttgccgga attcctgaag ccattctccg caggcatgat
      601 aaccagagac aaccggagca gttataccag agaaatgcca caggataagc atcttgtcgg
      661 caagatattt acccggcgca ttgagcgcaa caatctgaca cttcgtactc acatcaaacg
      721 acctgcccgg aaaacgattt gctttttacg ctccctggaa attcacgaaa agccattggt
      781 tcatttatcg aaaacacatg tactactgac aggagtcatt accagggcct cttttgctgt
      841 ttttttgcca tgaatgataa tgagaatcga cagcgataag ggaaaagtaa aataacacta
      901 atgctgtcgt tttctatcat attgattttt aattatttat tgtctttttt ctcaacccat
      961 ttttttacgc agacaaattt acccggggaa tacaaactgt aacgacagtt caccacacta
     1021 tgggggataa aatgccagat caatccggac actgcaggac tccttcacgc atcttacgga
     1081 tgcctgaact gagccaattg ttaggtatat ctcgttcgac aatttacgaa aaaatgaatc
     1141 cgttatcgaa atactacgat gcaaccttcc ccaggccggt acgtcttggt agtggttctg
     1201 tgggctggcg ttcatcagct attgacgaat ggcttacttt acatactgta cctgccagat
     1261 cagcagtcaa aggtgttaat gacgaagtga gacatacgct ttcttcagat atgaccatga
     1321 gcagataagt ttttgtcatc acgtgatcac ctcaggagga aaaagaagca taagaacaat
     1381 ttagccagaa gtttaattga taaacagaat ttccagcatt cattgttgaa agagcagcta
     1441 ttgtcgctat tgtagcggta attgctgcta taaccgaaac gtattacgaa aaaggaagca
     1501 tttatgcctt acatcaaacc ggatatgtcg tattcagtat atgcatgacc atcaatgata
     1561 gggaaggcaa taattaaagc tcaatacaat aaacaagtac ctctgactct ggttgcaatg
     1621 tcagtattaa cagcgatgtc aattgcctgt cagaatcagg ttgacgtatg cagtcccgga
     1681 aatttacgtg gggccgttaa tatttacact atggttctgg agaaagggaa aagaccgggg
     1741 gaaaggtatt agtgaaataa tttccggagg tattaagaat gagaaatatg aatgatattt
     1801 cgtggtgaat aatacagtct tcactgaatt aaccttatcg gtttattaaa catcagatct
     1861 acaaatgctc tgtatttctc agagcatcag gtctgaatag cagctatttt cgctattgta
     1921 gcgggatttg ctgctatttt actaacccat taatgaaaag agaggctttt atgtgcttac
     1981 ttgcaccaga aaatccttat ccgatatatg cactaccacc gctggtgaga aatgcaataa
     2041 ttgaaactca aaaaaataca caggcacctt tggctatggt ggcaacatcc gcattaacag
     2101 cgatctcaat tgcctgtcag aatcagattg acgtgtgcag acctggaaat ttacatgggc
     2161 ctgttaatct ttactctctg attctggctg attccggtga aaggaaaacg accgtggata
     2221 aagtgtttat gaaagcattt tatctcaggg atgaagccct ggcggatgaa tacgcgaaac
     2281 tggttgagaa ttacagtaca gaaaaggaaa tatgggagca aaaacaaaaa gcgctggaat
     2341 caaaatttca taaagagatt cgtgccggta aagattacaa ggcaacagaa tcagagcttg
     2401 aaacgcatct gaataaacct cctgttccgc cgcagatacg tcgaacaatt tttaatgaga
     2461 caacgataga gggaatgtta aaatattact ccgatagcaa tcgctctttt gctcttgtat
     2521 ccagtgaagg gggggtaatt tttgacagcc gggccatgag taaactggga attattaaca
     2581 ctttgtggga tggaggttct cttttcatcg acaggaaatc atctcccgga attaatttga
     2641 aggagccaag actgacgata tcggtgatga ttcagcctga tgtttaccac aaaggttttt
     2701 gtacgcgaaa aaaagaaatt gtgaaaacgt caggacatca tgcaaggttt ttgatgtgtc
     2761 aaccaacatc aacgcagggg acaaggataa tgatcaatga gcttattgat gaaagtctgg
     2821 cgatgagtgg tgaacgacgt tgccttcact tttctcctca ggctgccaga atctggacgg
     2881 attattacaa tgatgtggaa tcgaagctgg ggggattggg gcctttaaga cattgccggg
     2941 aatatgctgc taaaaatgca gagtatatgg caagactggc aggacttatt taccattcga
     3001 gcggtgaaga gggggagatt tccccttaca ctgcagaaat ggcgagagaa ttagcaatat
     3061 ggtacggtaa tgagtatgtg cggttgtcta atccgttaac ttttgacaac tctgccctga
     3121 ccgtacctgt gcgactcatt ccagaggagc ttgaactttt caactggata aaaagctatt
     3181 gtattgagaa ggggatcctc tgtatgaaaa aaaatgacat tttacagcgt ggtccgaacc
     3241 gtttccggaa aaaggataaa atcaactggt tactggattt actgtatgaa caaaacagag
     3301 ttgtaccggt tattgaggga aaaacgttgt gtgtcgcacc taactttgac ctctgaacga
     3361 cttaatcgtt gagtacatat agtctcctct gctacttgcg gaggagacta atgtctttac
     3421 catgtcagcg ttgtattaga aaactctggt acaggtcagt cagtaaatat aactggtgta
     3481 accactattt actattggat agagtgttgc tcaggatatt gtcggcttta gtctgaggtg
     3541 aggtggggcc ttcattgttt tcgtgttcaa taaataattt aggttaatta gtattattag
     3601 tggaagttat gtgatctgtt atgttgtctt gtacgcactt aacactggtt acgtaccgga
     3661 acactattag agtatgttat ggctctgtta cagtttatag gtatgaaaaa atataatctt
     3721 tcctgtatga aataatattc ttatggtgtg tggggtatgg tctgtatacc atcattttta
     3781 tggtgaagtt aaatatctgt agaggcctta agctgggtcg ctgtttttac ttaatggtac
     3841 agaagtatta atagcggtgt tgcctgtggc tacccgatac tggcttttgt gaatgtcaga
     3901 ttacttcctg taagtgattg aatggtactt ccttaaactc aattctatga ggtgtgtttt
     3961 taccattgta attcgtactg gtcctcagtt taagtgagtc ttactgtact ctgaaatatt
     4021 acctctttgg agtaatgagg taatacgaaa cagttttatc aggttgtctg tgctgctggt
     4081 tacctcgttt attaatgccg cggtacggcg tttctgtact ctcagtattt atgtaattcc
     4141 accttcctgt aacttttata aagtaaggac tataaaatgt ataatgctaa tcccaattac
     4201 gaaatggact tcatgattct gaaagacgtg aatgaacaca tggaaggtct gtttcaacgt
     4261 ttttctaaac tgttaccttt cagaattgat tttgcctaca ggaaagacac gcccagtttt
     4321 ggtcattcct gtaggcacag tatgtgtatt gaaatgcacc gcttacttag tgaaacccaa
     4381 acgatgctgg cgggatatta ctgggtaatg gaatatacgt caaacaaggg acttcatatt
     4441 cattttatcg gctacctgga tgggcaacgt cacaacaaat cttatcggat atccaggcaa
     4501 ctgggagata tctggaggcg gatcacggaa ggggagggtt attttcacct gtgcagggct
     4561 aaagacaaat acccggtgcg tatcgatcac gtaattcact atagtgataa atctgcggta
     4621 gacgacttac gttacgccct tagctatctg gccaaacagg atcagaaaga acacggtatt
     4681 attctgaggc gtagcaggct tcccgaaaaa agtaaccggg gccgaccacg acataactga
     4741 attacagctg tattaggtat tagaattaag tttctgtaag ttgttgggag gtgcttgagt
     4801 taataggcag aagagggcgg ggcgctttgt catcagtgta ggggacccga atttctgccg
     4861 tggtgcctca gtggatcgga tacaggtaac gaatatggtt cataaatctg acagtgatga
     4921 attagctgca ttgagggcag aaaatgcccg tctggtctca ttacttgaag ctcatgggat
     4981 tgaatggcga cgtaaaccgc agactcctgt gcagcgcgtt tctgtattat ccaccgacga
     5041 gaaggttgca ttatttcgtc ggttgtttcg tgggcgtgat gatgtatggg cacttagatg
     5101 ggaaagtaaa accagcggca aatcagggta ctctcctgcc tgcgctaacg aatggcaggc
     5161 gagaatatgc ggtaaacccc ggataaaatg tggggactgc gctcaccgtc agttgattcc
     5221 tgtatccgat ctcgtcatct accaccatct ggccggtacc catactgtcg ggatgtatcc
     5281 gttgctggaa gatgattcct gttattttct ggcagttgat tttgatgaag ctgaatggca
     5341 gaaggatgca tccgcattca tgcgatcctg tgatgagctg ggtgtacctg ctgcgctgga
     5401 aatatcccgt tcacgtcagg gggcgcatgt ctggatattt tttgcctcac gagtttcggc
     5461 ccgcgaagct cgccgtctgg ggactgctat tattagctat acgtgtagtc ggacccgaca
     5521 gctgcgattg gggtcttatg accgattatt tcctaatcag gatactatgc caaaaggggg
     5581 atttggtaat ctcatagcgt tacctctgca aaaaagaccg cgtgaattgg ggggaagcgt
     5641 ttttgttgat atgaatctcc agccttatcc tgatcagtgg gcttttcttg tatcggtgac
     5701 cccgatgaat gtgcaggata ttgaaccgac gatattacgg gctacaggga gtatccatcc
     5761 tctggatgtg aattttatca acgaagaaga cctgggtacg ccgtgggaag agaaaaaatc
     5821 atcaggaaac agactgaata tttctattgc agaaccgctg aaaatcacgc tggcaaacca
     5881 gatctatttc gaaaaagcgc aattacctca ggtgctgatt aaccgactta ttcggctggc
     5941 agcatttccg aaccctgagt tttataaggc tcaggcaatg cgtatgtcag tctggaataa
     6001 gccccgtgtt ataggttgtg cggagaatta cccgcaacac attgcgttgc cccggggatg
     6061 tctggacagc gtattatctt tccttaggga caacaatatt gctgcagaat taatcgataa
     6121 acgatttgcc gggacggaat gtaatgccgt ttttatggga aacctcagag cggagcagga
     6181 agaggccgtt tcggcattac tccgttatga cactggtgtg ctttgtgcgc caacggcttt
     6241 tggtaagaca gttaccgcag cggcagtgat tgccaagcgg aaagtgaata cactgatact
     6301 ggtacaccgg actgaattgc tgaagcagtg gcaggagcgt ctcgcggtgt ttctgcaggc
     6361 cggtgacagt attggtatta tcgggggagg taaacataaa ccctgtggca atattgatat
     6421 tgcggtggtg cagtccatat ccagacaagg agaagttgaa cctctggtca ggaattatgg
     6481 gcaaatcatt gtggatgagt gccatcatat tggcgcggtt tcattttctg cgattctgaa
     6541 ggaaacgaat gccagatatc tgcttggcct gacggcaaca ccaatccgac gggatggtct
     6601 gcatcccatt atttttatgt actgtggtgc cattcgccat acagcggtcc gcccgaagga
     6661 aagcccacat aatctggagg tactgatccg ttcccgtttt acatctggtc atttaccatc
     6721 ggatgcgaga atccaggata ttttcagaga aattgctctg gatcatgaca gaacggtggc
     6781 gatagctgaa gaagccatga aagctttcgg gcaggggcga aaagttctgg tactgactga
     6841 acgtacagat catctggatg agatagcatc agtgatgaat tcactgaaat tgtctccctt
     6901 tattctccat ggtcgactat cgaaaaaaaa gcgtgcgatg ctgatatccg ggctgaatgc
     6961 tcttcctccc gattctcctc gaattttgtt gtcaacaggc agacttattg gtgagggatt
     7021 tgaccaccct ccgctggata cgctgattct tgccatgcct gtgtcatgga aagggacatt
     7081 acagcagtat gcagggcgtc ttcacagaga gcataccggt aaaagcgatg tcaggatcat
     7141 tgattttgtg gataccgcgt atcctgtgct gctcagaatg tgggataaac gtcagcgggg
     7201 ttataaagcg atggggtaca ggattatagc tgacggcgat gaatcagtga tttaatttta
     7261 aggagtactt tacccttttt aaataatttg agctccccgg gttgttcggg caacaaagag
     7321 tcccagtata caggcttcag gaatatgttg tagtatcagg cttttcatcg ctttaaaatg
     7381 actaccggca gtgagaacgt catcaaaaat aacaatctag ggcctgcacc cttccagtaa
     7441 ttgttcatcc agttggtagt ttgccataat cgcttctggt aagggccggt ttgttgcggt
     7501 atgagaggct attgttgata cgttttgcac aaccagttcc cagaaatcca gccatggttc
     7561 gtaggtttgc gcaattcgca gaatttctgt catacggtca tcgtaagcgg catctgtacg
     7621 gcatttggat ggtggtacgg gaacaaatgt gtattcctca agattaccga tatttgatat
     7681 gagcatctga gcaacatact caatagcagc ctttttatag tggtaatcag gctacgaaag
     7741 tacagcgttt tataaattgt ctcaggtgat attcgcagcg ttttttgacg tggttttgtt
     7801 cgccttaacc atcctgatat ttgctctgga gaccatttca tctccagctt ttccagaaca
     7861 agctttcgca atggtaaatt ttgatccagt aagcacggtt ttggcctttt cgccattctg
     7921 ttggctcggt tattagcatc aacagctttg taatagcgtc tgccccgatt ac
//
LOCUS       DAZFTN010000174         7949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFTN010000174 DAZFTN010000000
VERSION     DAZFTN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(122..1609)
                     /gene="yeeO"
                     /locus_tag="ACHKV2_004648"
     CDS             complement(122..1609)
                     /gene="yeeO"
                     /locus_tag="ACHKV2_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6942392.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            1770..1845
                     /locus_tag="ACHKV2_004649"
     tRNA            1770..1845
                     /locus_tag="ACHKV2_004649"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1803..1805,aa:Asn,seq:gtt)
     gene            complement(1883..2833)
                     /gene="cbl"
                     /locus_tag="ACHKV2_004650"
     CDS             complement(1883..2833)
                     /gene="cbl"
                     /locus_tag="ACHKV2_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO6942393.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2935..3852)
                     /gene="nac"
                     /locus_tag="ACHKV2_004651"
     CDS             complement(2935..3852)
                     /gene="nac"
                     /locus_tag="ACHKV2_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO6942394.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4178..4253
                     /locus_tag="ACHKV2_004652"
     tRNA            4178..4253
                     /locus_tag="ACHKV2_004652"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4211..4213,aa:Asn,seq:gtt)
     gene            complement(4309..5244)
                     /gene="ldtA"
                     /locus_tag="ACHKV2_004653"
     CDS             complement(4309..5244)
                     /gene="ldtA"
                     /locus_tag="ACHKV2_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6942395.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5306..6385)
                     /gene="cobT"
                     /locus_tag="ACHKV2_004654"
     CDS             complement(5306..6385)
                     /gene="cobT"
                     /locus_tag="ACHKV2_004654"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO6942396.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6397..7140)
                     /gene="cobS"
                     /locus_tag="ACHKV2_004655"
     CDS             complement(6397..7140)
                     /gene="cobS"
                     /locus_tag="ACHKV2_004655"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO6942397.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7137..7682)
                     /gene="cobU"
                     /locus_tag="ACHKV2_004656"
     CDS             complement(7137..7682)
                     /gene="cobU"
                     /locus_tag="ACHKV2_004656"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO6942398.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCSAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7834..>7949)
                     /locus_tag="ACHKV2_004657"
     CDS             complement(7834..>7949)
                     /locus_tag="ACHKV2_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO6942399.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2194 a         2162 c         1723 g         1870 t
ORIGIN      
        1 taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat gcccacccac
       61 aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg cattttgtcg
      121 tttattccga cacagctggc tttttttcac acttttgcgg ttcgggtcga gggtatttcc
      181 acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca gcccagtcgg
      241 caaacattcc catccagaca ccaaccacac cccagccaag catgatgccc agcacataac
      301 cgacgacaac ccgacaaccc cacatgctca acatagaaac ccacatagcg taacgggcgt
      361 cacgagcccc cttaaatcca gcgggtagca cccatgaagc ggaccaaata ggcataaata
      421 aagcatttag ccaaatcaga atcacaacga catgtttaac ctgtggatcc tgggtgtaaa
      481 acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt aatccaagag
      541 tggtcagcca aaacacatgc cgcaactgaa tctctgcttg cgctatctgc cctaccccca
      601 accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt aagttgataa
      661 gagccgcaat tgaaaacgcg ataaaatttc cggcaataac actggtcccc attccggcaa
      721 cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca ctcgctggaa
      781 taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa tagctcttta
      841 acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt gcaactgcgc
      901 caatataacg agaaatggtt aaacccaacc cagccccgac aaatcccagt cccggccagg
      961 agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt
     1021 taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta ccaattagag
     1081 tgatggcagc tgctggataa ctgagtaccg tcagctccag ataagtcaac gccagtgctt
     1141 taacttctgt cgtggcatca cccgcgacga aatcaataat ttgctcgcca aaatgatgaa
     1201 taagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca
     1261 ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc cacaacgaca gtagtaccaa
     1321 gatcgatagc agcaaaaaaa gccataatga ccatattgaa gctgtccgcc aatcccacgc
     1381 cggccatcgc atcttttccc agccagctga ccagaaaagt gctcagaacc cccatcaaca
     1441 ggacacaggc attctccatg aagataggaa cagcaaacgg ggttatctcg cgccagaaca
     1501 acactttgta gctcttgcgt ttagcgtgcc agcgagtgcc gtgaacaacc tggcgtaaag
     1561 cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaataatg
     1621 agggagaatc agcaaagctg caaagatttt cgccatcaaa ttgtctgcaa atgcaacaaa
     1681 ctgttgatag aaacggcaaa cagttgggaa aattaaaaat cgggtttgac aaaagatttt
     1741 tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga
     1801 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc
     1861 cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca
     1921 tcacctgccg cttgatatcc tcaactgaaa gtcctgcatt gcaaagttcc agaaagcgcc
     1981 agacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga
     2041 aaagatgccg cgtatccagg cggattaaat tcttttcctc ttgttcaccg ctggattgtt
     2101 cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct
     2161 gcgcacttaa tataatatct gccagcaaac ctttgcgggc aaatgcgtca tcaatacgtg
     2221 agcgccccgt aatcccctgt cggtaagtga ttaacggcca cttcgctatt gattccagcg
     2281 tcaatggtgt aatttgcgtc aagggatgat cgtgtggaac aagcaaacta tggtgccaac
     2341 gaaaccacgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa
     2401 tatcagcttc accattatgc aacaacgtcg caatttcctg tggcgtcccc tggattagct
     2461 cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac
     2521 gtgcctgagt atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg gtaaacaggt
     2581 ctgcaagccg acgaacatta ctggcttcat tgagaatacg ttctgcaatg accagtaatg
     2641 ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat atttcgatgc
     2701 caagttcatc ctccagttcc cgaatatgac ggctgacgcc tgactgtgag gtaaaaagca
     2761 tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ttcgcggatt atctttagtt
     2821 gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaac
     2881 gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct
     2941 caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc
     3001 tttcactgcc tgtgcctgtg gcgataagtt cgctctggcg ggtaaattta atgacaaaga
     3061 gagactcatg gaaggcgtgg taatgcgtga catccaccca tttactgcgc cacataacga
     3121 acgcgcggcc gattcgggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag
     3181 cgtagcaata gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaacgc
     3241 ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgtgc
     3301 aatagcatta acatcaacgc tttgccccgg gcaatcttga gttcctacta ggaaaagatc
     3361 ttctttcagc aaagcctgac tggatacacc agccacaggg gaatgctcat aaatcaccgc
     3421 catatcgagt tggtgattta tcagtttttc gttaagcact gcaccgctat tttcatgaag
     3481 atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga
     3541 tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg cctgaccaac
     3601 gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgcg catgggtata
     3661 gagaattttt ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagttgttg
     3721 atttaactca ccttcgagtg tggcaacctg ctggctgagc gctggttgtg cgatatgcaa
     3781 tacttcagca gcctgagtca ggctaccaat atctacaatt tttacgaagt atttcaggcg
     3841 tctgaagttc atgttgcctc cggtttttga gaatcggccc aagtgccgcc attacttaca
     3901 accagattgc aagatgcttg ccagttttat tttggcgttg atgtacaagc taaccaactg
     3961 tcaaataaga gattatgata gattcgtcat tggctctttt caccagcagt cgcgttcccc
     4021 tgccctataa aaggagggta tgcaccacga tggttcatta ccaataagat tgaaatctca
     4081 tcactttgtt gaaattgaca gcaaacaaac aaaaaaatgc atttcaccct ttgacatcac
     4141 catgcactgc cattaatatg cgccccgttc acacgattcc tctgtagttc agtcggtaga
     4201 acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcaa
     4261 aaaagcctgc tttctagcag gctttttgct ttctaattac caacgctctt aaaacatctg
     4321 tcttgaacca gaactaattt gcacaggcat tcccgatcga cgttgcaacg cagcatttgc
     4381 gcgattcaca tcaacttctt gcccgttgat aaacgcccgc aaagatgggg ttaccggcaa
     4441 tggcactttt cggtcagact catattcagc acgattgcgc gacagcggct catgaacttc
     4501 cagccacttt gaaccatccg gttccgttgt gtatttcact ggctgatcaa taatttgcac
     4561 acgagtacct acaggaacat tatcaaacag atatttgata tcgtcattgc gcagacgaat
     4621 acagccctga cttacccgga gcccaatacc aaaattggca ttggtaccgt ggatggcata
     4681 tagcctgcca atataaatcg cgtacagccc catgggatta tcgggccccg caggaacaaa
     4741 tgcgggcaaa ctctcccctc gtttcgcata ttcgcgccgg gtgttcggcg ttggcgtcca
     4801 ggttggtgct tcttgtttac gttcaacggt agtcacccag ttacgcgggg tttctcgccc
     4861 agcctggccg ataccaatag gaaagacttc cacagtatta ctgtctggtg ggtagtaata
     4921 aagacgcatc tcagcgacgt taaccacaat ccctttacga acagtgacgg gcaaaatcag
     4981 ttgctgcgga atggtgagtt gcgaacctga cttcggtaag aagacatcag cgcccggatt
     5041 cgcttccagc atgttactta acccttgccc gtattgtgcg gcaaaagtct ccagcggctg
     5101 ggtattgtga tcaggaacag ttacagtaag cgactgcccc actaaacggc taccctctgg
     5161 aggtaatgga taagttaccg ccaggctagt atggctggca aaaagcagag caaatgagca
     5221 aagaatattt acacgacgca tcatgtccct ttcctaagtc gcgaaagcta tcggttaagt
     5281 atagctttta tcagactttt cgtttttaac tgttcaaatc agaagtcgta ttccccggta
     5341 gaacaatttt actggcagca agttcgccca tgttgttgta tatcgcacaa gcagcttcga
     5401 tgatgggcat cgccagagct gcgccactcc cctcacctaa acgcatatcc atattgagaa
     5461 aaggctccag ccccaaatgc gacagcgcta tacgcgcccc tttttctgcc gacaggtgag
     5521 aaggaatgag atacggtttg attgcaggag acatctggca ggctgcgagc gcagcagcat
     5581 aagaaagaaa tccatccagc aacacgggta aaccgcagga agcagcgcct aacatcacgc
     5641 cagccattcc gaccaaatca aatccaccca cttttgccag aacattaacg ccatcctgcg
     5701 gatttggctg attcaacgta atcgcccggc gcacaacatc aattttatta gccagtttat
     5761 ctgtcggcag gtttgcgcca atcccaacca cttcttcagg atcccggcca gtgattgtgc
     5821 tgactattgc tgctgccggt gtcgtgtttg ccatccccag ttcacctaca ccaaacagcg
     5881 tgacaccgtt ttttgccagc tcccgcgtat aacatatgac gtccaaaagt aacttttcag
     5941 cctgacggcg actcattgcc ggagctgaag caatattgcc gctacctcgt gcgacacgca
     6001 tgttgataag cccggggata ggctcagcag tatcaatacc aacatcaatt acgtggacgt
     6061 tagcgcccgc ttgtgctgcc agcacacaca cgccggttgt tccgcgggtc atattttcag
     6121 cctgaatggc tgtcacttct tttggggaaa tagcgacccc ttcctcccag acgccgtgat
     6181 cggcacacat aaccagtacc gcttttttgc ccacatgcgg tatgccattc aaccccggca
     6241 ttcccgccag ttgtacgcca agcgcctcca gccttcccag gctaccaaca ggtttgagta
     6301 acccgtcaat atgccgttgt gcacgcgaca tagcggcagg atcgatggca gggatcgtat
     6361 tcagtaaatt ggcaagtgtt tgcatgtcac gtctctttat agcagtgcca gcaggaagac
     6421 cagttcacca agctctatgg ctgctcccag cgtatcgccg gtttgcccgc ccagcgtacg
     6481 tttgagaagc tgaccgagga tgaaaatcgc caccatcgtc accaccatag cagccacacc
     6541 atgcataccc ggcaataata cggcagcaaa aatagcggct aagccgagtg tgacgcaggt
     6601 ttgtcgccca tcaattttgc caatgaacac attgccaagc ccttcttccc gggcataacg
     6661 atgacgatac atcaataatg cggcagtccc gcggctgacc gcacatgctg ccgccagcga
     6721 tgcaaggatc ggttcgccac gcagagccaa ctcgcttaac accaaaatct ttgccagtac
     6781 aacaaaaatc aatgccagac cgccgtgggt acctaaacgg ctatcacgca tgatttccaa
     6841 catgcgatcg cggctacgtg cagaaaatac gccatcacag gtatcagcga ggccgtccag
     6901 atggaatccc ccggtcatca gcaccagcac aagtacgcta aacagtgccg ccagtggtgc
     6961 gccacaccat gcctgcagca ccatgaagac cagcccgcta atcgcgccaa gcaataatcc
     7021 aatcaaagga aaagtaataa taccgcgaga ataatgctcg aaatccagtc cctgggacca
     7081 gcgacgcggt acaggcagac gcgtaataaa tgagagcatc gcccaaaata atttactcat
     7141 ttgattttta ctccaatacc cgaaaccacc agccatactt catttgccgc agcggccaac
     7201 tgctgattta cccgcccggc aatatcacga aaatgtcgtg ccagacgact ctccggcaca
     7261 atccccattc ccacttcgtt agtcactaat acaacctttg cggagcaacg ttggcaggca
     7321 gcaatcaacg actgaatctc agcattaatc gcctgttcca tcgcctgata atcccattca
     7381 tcagggtctt tatcgccgcc ataatcaaac aacagattag tcaccattgt ggtaacgcat
     7441 tcaagcaaca caacctcatt cgggttaatg tctgcatgaa ttaattcatc aagatgttgc
     7501 cagcgctcca ctgtgcgcca gtgctccggg cggccttgcc gatgatgttc tatccgtgca
     7561 gccatctcat catcaaggat ttgcgaggta gcgatataca gaacctgtga agagtcccca
     7621 ataagcgcct ctgcgtggcg actcttcccg ctccgtgcgc cccccgtcac caaaatcatc
     7681 ataccgactc ctgattcaaa tgtatgatgg tattcattga acagaacaat ggatccccca
     7741 ccgttgctga ccgcaaaaca tttacgcgat taatactgcg cgtaatataa ttaaaaatcc
     7801 agcattctca atccattacg aaataagatg gcattacgga atagccagac tttctcgtgt
     7861 taacgtccga caaaatgcgt cattgacagc ggtttctgtg gattgacaca tttcattcag
     7921 ataatgaatt aatgctactg ccggaacaa
//
LOCUS       DAZFTN010000175         7797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFTN010000175 DAZFTN010000000
VERSION     DAZFTN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(204..1451)
                     /locus_tag="ACHKV2_004658"
     CDS             complement(204..1451)
                     /locus_tag="ACHKV2_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO6942400.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1523..2437)
                     /locus_tag="ACHKV2_004659"
     CDS             complement(1523..2437)
                     /locus_tag="ACHKV2_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6942401.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            2653..4086
                     /gene="cscA"
                     /locus_tag="ACHKV2_004660"
     CDS             2653..4086
                     /gene="cscA"
                     /locus_tag="ACHKV2_004660"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6942402.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(4094..5089)
                     /locus_tag="ACHKV2_004661"
     CDS             complement(4094..5089)
                     /locus_tag="ACHKV2_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6942403.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            5332..5550
                     /locus_tag="ACHKV2_004662"
                     /pseudo
     CDS             5332..5550
                     /locus_tag="ACHKV2_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            5568..6896
                     /gene="dsdA"
                     /locus_tag="ACHKV2_004663"
     CDS             5568..6896
                     /gene="dsdA"
                     /locus_tag="ACHKV2_004663"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO6942404.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(7004..>7797)
                     /gene="emrY"
                     /locus_tag="ACHKV2_004664"
     CDS             complement(7004..>7797)
                     /gene="emrY"
                     /locus_tag="ACHKV2_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6942405.1"
                     /translation="ISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIV
                     LMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRVLVTFSFLMYAVC
                     YYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFF
                     RTLLGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSSSQIMDKYYGSLSGVLNE
                     INNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
BASE COUNT         2192 a         1923 c         1828 g         1854 t
ORIGIN      
        1 tgatacacgg gacaggtcgc agaatgacga caatgtcctg gaaatcagcg aaccgtgtat
       61 ccggagtaca tttgagcgac tgtaccagaa catgaatgag gtgtttggat taggcgatta
      121 ttagcagggc taagcatttt agtattatta ttttccggtt gaaggatatg gagatatcga
      181 caacaaccgg aaaaagttta cgtctatatt gctgaaggta caggcgtttc cataactatt
      241 tgctcgcgtt ttttactcag gaagaaaatg ccaaatagca acatcaggca gacaataccc
      301 gaaattgcga agaaaactgt ctggtagcct gcgtggtcaa agagtatccc agtcggcgtt
      361 gaaagcagca caatcccaag cgaactggca atttgaaaac caatcagaaa gatcgtcgac
      421 gacaggcgct tatcaaagtt tgccacgctg tatttgaaga cggatatgac acaaagtgga
      481 acctcaatgg catgtaacag cttcactaat gaaataatcc aggggttaac gaacagcgcg
      541 caggaaagga tacgcaacgc cataatcaca acaccgataa gtaatgcatt ttttggccct
      601 acccgattca caaagaacgg aataatcgcc atgcacagcg cttcgagtac cacctggaat
      661 gagttgagat aaccatacag gcgcgttcct acatcgtgtg attcgaataa acctgcataa
      721 aagacaggaa aaagttgttg atcaaaaatg ttatagaaag accacgtccc aacaataaat
      781 atgacgaaaa cccagaagtt tcgatccttg aaaactgcga taaaatcctc tttttttacc
      841 cctcccgcat ccgccgctac gcactggtga tccttatctt taaaacacat gttgatcatc
      901 ataaatacag cgccaaatag cgagaccaac cagaagttga tatggggact gatactaaaa
      961 aatatgccgg caaagaacgc gccaatagca tagccaaaag atccccaggc gcgcgctgtt
     1021 ccatattcga aatgaaaatt tcgcgccatt ttttcggtga agctgtcaag caaaccgcat
     1081 cccgccagat accccaggcc aaaaaagagc gcccccagaa ttagacctac agaaaaattg
     1141 ctttgcagta acggttcata aacgtaaatc ataaacggtc cggtcaagac caggatgaaa
     1201 ctcatacacc agatgagcgg tttcttcaga ccgagtttat cctgaacgat gccgtagaac
     1261 atcataaata gaatgctggt aaactggttg accgaataaa gtgtacctaa ttccgtccct
     1321 gtcaacccta gatgtccttt cagccaaata gcgtataacg accaccacag cgaccaggaa
     1381 ataaaaaaga gaaatgagta actggatgca aaacgatagt acgcatttct gaatggaata
     1441 ttcagtgcca taattacctg cctgtcgtta aaaaattcac gtcctattta gagataagag
     1501 cgacttcgcc gtttacttct cactattcca gttcttgtcg acatggcagc gctgtcattg
     1561 cccctttcgc cgttactgca agcgctccgc aacgttgagc gagatcgata attcgtcgca
     1621 tttctctctc atctgtagat aatcccgtag aggacagacc tgtgagtaac ccggcaacga
     1681 acgcatctcc cgcccccgtg ctatcgacac aatccacaga cattccagca aaatggtgaa
     1741 cttgtcctcg ataacagacc accacccctt ctgcaccttt agtcaccaac agcatggcga
     1801 tctcatactc ttttgccagg gcgcatatat cccgatcgtt ctgtgttttt ccactgataa
     1861 gtcgccattc ttcttccgag agcttgacga catccgccag ttgtagcgcc tgccgcaaac
     1921 acaagcggag caaatgctcg tcttgccata gatcttcacg aatattggga tcgaagctga
     1981 caaaacctcc ggcatgccgg atctccgtca tcgcagtaaa tgcgctggta cgcgaaggct
     2041 cggcagacaa cgcaattgaa cagagatgta accattcgcc atgtcgccag cagggcaagt
     2101 ctgtcgtctc taaaaaaaga tcggcactgg ggcggaccat aaacgtaaat gaacgttccc
     2161 cttgatcgtt cagatcgaca agcaccgtgg atgtccggtg ccattcatct tgcttcagat
     2221 acgtgatatc gactccctca gttagcagcg ttctttgcat taatgcacca aaaggatcat
     2281 cccccacccg acctataaac ccacttgttc cgcctaatct ggcgattccc accgcaacgt
     2341 tagctggcgc gccgccagga caaggcagta gccgcccgtc tgattctggc aagagatcta
     2401 cgaccgcatc ccctaaaacc catactttgg ctgacatttt tttcccttaa attcatctga
     2461 cttacgcata gtgataaacc tctttttcgc aaaatcgtca tggatttact aaaacatgca
     2521 tattcgatca caaaatgtca tagttaacgt taacatttgt gatattcatc gcatttatga
     2581 aagtaaggga ctttattttt ataaaagtta acgttaacaa ttcaccaaat ttgcttaacc
     2641 aggatgataa aaatgacgca atctcgattg catgcggcgc aaaacgcact agcaaaactt
     2701 cacgagcgcc gaggtaacac tttctatccc cattttcacc tcgcgcctcc tgccgggtgg
     2761 atgaacgatc caaacggcct gatctggttt aacgatcgtt atcacgcgtt ttatcaacat
     2821 cacccaatga gtgaacactg ggggccaatg cactggggac atgccaccag cgacgatatg
     2881 atccactggc agcatgagcc tattgcgcta gcgccaggag acgagaatga caaagatggg
     2941 tgtttttcag gtagtgctgt cgatgacaat ggtgtcctct cacttatcta caccggacac
     3001 gtctggctcg atggtgcagg taatgacgat gcaattcgcg aagtacaatg tctggctacc
     3061 agtcgggatg gtattcattt cgagaaacag ggtgtgatcc tcactccacc agaaggaatc
     3121 atgcacttcc gcgatcctaa agtgtggcgt gaagccgaca catggtggat ggtagtcggg
     3181 gcgaaagacc caggcaacac tgggcagatc ctgctttatc gcggcagttc attacgtgaa
     3241 tggactttcg atcgcgtact ggcccacgct gatgcgggtg aaagctatat gtgggaatgt
     3301 ccggactttt tcagccttgg cgatcagcat tatctgatgt tttccccgca gggaatgaat
     3361 gccgagggat acagttaccg aaatcgcttt caaagtggcg taatacccgg aatgtggtcg
     3421 ccaggacgac tttttgcaca atccgggcat tttactgaac ttgataacgg gcatgacttt
     3481 tatgcaccac aaagctttgt agcgaaggat ggtcggcgta ttgttatcgg ctggatggat
     3541 atgtgggaat cgccaatgcc ctcaaaacgt gaaggctggg caggctgcat gacgctggcg
     3601 cgcgagctat cagagagcaa tggcaaactt ctacaacgcc cggtacacga agctgagtcg
     3661 ttacgccagc agcatcaatc tatctctccc cgcacaatca gcaataaata tgttttgcag
     3721 gaaaacgcgc aagcagttga gattcagttg cagtgggcgc tgaagaacag tgatgccgaa
     3781 cattacggat tacagctcgg cactggaatg cggctgtata ttgataacca atctgagcga
     3841 cttgttttgt ggcggtatta cccacacgag aatttagacg gctaccgtag tattcccctc
     3901 ccgcagcgtg acacgctcgc cctaaggata tttatcgata catcatccgt ggaagtattt
     3961 attaacgacg gggaagcggt gatgagtagt cgaatctatc cgcagccaga agaacgggaa
     4021 ctgtcgcttt atgcctccca cggagtggct gtggtgcaac atggagcact ctggcaactg
     4081 ggttaacata atatcaggtg gaacaacgga tcaacagcgg gcaagggatc cgcgtcacac
     4141 ttcccccttc acgaccttca ataatatgca atgcagcttc ccgcccgata atgtcatgtg
     4201 gaagctgaat tgtggtcagc ggcggtaaaa acagatgccc gacgccaacc agattatcaa
     4261 agcccattac ggcgacatcc tgcgggatac gtaccccctt agccaggaga acctgataag
     4321 ccacaaaggc tgcgcgatcg ttaccacata tcagaacatc aaaatctggt ttgcccgatt
     4381 tgaagtggtc attgagtaaa cttgcgagat cggtgtagtg atcatcacct gttgccatgt
     4441 gaaattgttt cacctcagcc agatctcgtc cagcatcacg ccaggcctgc tcaaatccct
     4501 gccgacgata ccctgttgcc aacgcacttt ccggtagcca gaagcataac ggttgacgat
     4561 agcccgccgc gagcaaatgc tgtgttgatt catattgtgc agtgtaatca tcagggatat
     4621 aactgggtaa cgctgggtca tccgccacac agttcgccaa tacaatattt tcaccataca
     4681 gagactcagg tagcgtgata tgtcgcagcc ccattgtagt atagataatg ccatccggac
     4741 ggtgggcaag cagctgacgt gccgcgcggg cagcgtcatc ttcagaaaaa atattgatta
     4801 aaaaactatt ccagccgaac tcgctggcgg tttgctcaat ggcaagcaga atatcaacag
     4861 agaaaggagt ggtagcagtg tcctgcgcca gcacggcgag agtcgacggc ttacgtcctt
     4921 gagcgcgcat cttacgggcg gaaagatcag gaacataatt cagggtctgg attgcctgca
     4981 atacgcggtc acgcgttgca ggacgcacag attctgcatt atgcatcacc cgggagactg
     5041 tcatcatcga cactcccgcc aggcgtgcga catcctttaa tgaagccata cccaagccgt
     5101 ttgccgtaaa acgggcactg tagcagaaac agacgtcact ggcgagatcc aacgaaattc
     5161 tcgacgccct atcacccgac acggcaatac aataaaaaat aacaataatt gccggataat
     5221 tgtacccaat tccgcctctg ttctcgccaa cgagtgacca tataggcaag taagaaaaac
     5281 atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgccg
     5341 ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg cgcgattggc
     5401 tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg cgctacgctc
     5461 aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg
     5521 ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta
     5581 aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa
     5641 ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga
     5701 ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa
     5761 aagcatttcc tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc
     5821 cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg caactgttac
     5881 tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc gggatttatg
     5941 aagtcctggc acatgcagaa aaactggctc tggaagcggg gttgctgacg cttgaagatg
     6001 actacagcaa actgctttct ccggagttta aacagttctt tagccagtac agcattgctg
     6061 tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta
     6121 aggtgacagt gcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc
     6181 atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta
     6241 aagcagcgca gtctgacccg aactgtttct ttattgatga cgagaattcc cgcacgttgt
     6301 tccttgggta ttccgtcgca ggccagcgtc ttaaagcgca atttgccgag caaggtcgta
     6361 ttgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg
     6421 gtggcgtcgc attcggactt aagctggcgt ttggcgatca tgttcactgc ttttttgccg
     6481 aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt
     6541 ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg
     6601 catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg
     6661 atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac
     6721 cttcggcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac
     6781 agatgcacgg tttcagcgca gaacaactgc gtaatgccac tcatctggtg tgggcgacgg
     6841 gaggtggaat ggtgccggaa gaagagatgg agcaatatct ggcaaaaggc cgttaataac
     6901 gtttcaacgc agcatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact
     6961 taagatcccc agtcttaccc gctataaccc cctgctttta ccatcaccca acgcctttcg
     7021 ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg
     7081 ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa
     7141 tttcattaag aactcctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat
     7201 taaacacggg gttaaattga tcgatggttt ctgtcaactg actatggtgt aacgattcgc
     7261 gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctaacaag gtacgaaaaa
     7321 aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg
     7381 ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg aaaaaactgc ggcaaaatga
     7441 tacctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat
     7501 acatcaaaaa actgaatgtc accaacaccc gcatgtctat tttgttgcca taacgtccta
     7561 tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc ataagcaagt ccggcccata
     7621 tcgcattata ccccatcgtt tcctggagta actgcggcat aaggacgatc gctccagagt
     7681 aaaataaata cgcgcatgtg atactcacaa taccaatggt gaagttacgg gacttaaaca
     7741 aactgagatc aagaatcggg ttctctgagg tcgactccca aatgactaaa gagatca
//
LOCUS       DAZFTN010000176         7740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFTN010000176 DAZFTN010000000
VERSION     DAZFTN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1386
                     /locus_tag="ACHKV2_004665"
     CDS             <1..1386
                     /locus_tag="ACHKV2_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFO6942406.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
     gene            1406..2152
                     /gene="traX"
                     /locus_tag="ACHKV2_004666"
     CDS             1406..2152
                     /gene="traX"
                     /locus_tag="ACHKV2_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO6942407.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2211..3071
                     /locus_tag="ACHKV2_004667"
     CDS             2211..3071
                     /locus_tag="ACHKV2_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6942408.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3174..3734
                     /locus_tag="ACHKV2_004668"
     CDS             3174..3734
                     /locus_tag="ACHKV2_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO6942409.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3867..4079
                     /locus_tag="ACHKV2_004669"
     CDS             3867..4079
                     /locus_tag="ACHKV2_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6942410.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4324..4785
                     /locus_tag="ACHKV2_004670"
     CDS             4324..4785
                     /locus_tag="ACHKV2_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6942411.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4831..5040
                     /gene="hha"
                     /locus_tag="ACHKV2_004671"
     CDS             4831..5040
                     /gene="hha"
                     /locus_tag="ACHKV2_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6942412.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5078..5416
                     /locus_tag="ACHKV2_004672"
     CDS             5078..5416
                     /locus_tag="ACHKV2_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942413.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5656..5910
                     /locus_tag="ACHKV2_004673"
     CDS             5656..5910
                     /locus_tag="ACHKV2_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO6942414.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6146..6220
                     /gene="tap"
                     /locus_tag="ACHKV2_004674"
     CDS             6146..6220
                     /gene="tap"
                     /locus_tag="ACHKV2_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO6942415.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6213..7070
                     /gene="repA"
                     /locus_tag="ACHKV2_004675"
     CDS             6213..7070
                     /gene="repA"
                     /locus_tag="ACHKV2_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO6942416.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         1986 a         1818 c         2206 g         1730 t
ORIGIN      
        1 cggattgccg gtatcagcag ggatgacggg ctgataactc tgcaggatgc ggaaggtaac
       61 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacactgta ccccccggac
      121 accatcaggg tggggaccgg tgaccggatg cgcttcacga agagtgaccg ggggcgtggt
      181 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacgctgtcg
      241 ggcggacagc agacccgggt gattcgcccc gcccaggagc gggcagagca acatattgac
      301 ctggcctatg ccatcaccgc tcacggtgcg cagggggcaa gtgaaacctt tgccatcgcg
      361 cttgaaggta cggaaggcag ccggaaactg atggccggct ttgagtcagc ctacgtggcc
      421 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
      481 gccattaaca atgccgtaca gaaaggaaca gcccacgatg tgtttgaacc gaaaccggac
      541 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
      601 gcagggcgtg ccgttcttcg tcaggcgggg cttgccgggg gagacagtcc tgcacggttt
      661 attgctccgg gacgtaaata tccacagccg tatgtggcac tgccggcgtt tgaccgtaac
      721 ggcaagtctg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
      781 ggattcagtg gtgaaggacg ggtgaaaggc agcggggatg cgcagttcgt ggccctgcag
      841 ggcagccgta acggagagag cctgctggct gataatatgc aggatggtgt ccggattgcc
      901 cgtgataatc ctgacagcgg tgtggtggtg agaatcgccg gtgaaggtcg tccgtggaat
      961 cccggtgcca tcaccggtgg tcgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     1021 ggagccggaa atggcgagcc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     1081 gccatccgcc gtgaaacgga acgccgcgca gatgaaattg tccgtaaaat ggcagagaac
     1141 aaacctgacc tgccggacgg cagaacagag caggctgtca gggagattgc cgggcaggag
     1201 cgtgaacggg ctgtcacttc tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     1261 ccacaacggg agcgggaggc ggtccgtgag gttgtccggg aaaacctgct gcaggagcga
     1321 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cctgggcgga
     1381 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     1441 ttcactggct gcccggaccg atacctggtt gcagtcgttg ctggtctggt cacccggaca
     1501 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccacg caaaccgcat
     1561 tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagcctttc cgttgtttgc
     1621 tctggtgtgg gggctgaatc tgtcccgcca tgcgcatatc aggcagtcgg ccattaaccg
     1681 gctgtgggga tggggaatta ttgcccagtt cgcgtattat ctggcaggct ttccgtggta
     1741 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     1801 gcgcagtggg tggcgtacag cagcggccat tctcctgatg gcactgtggg ggcctttgtc
     1861 cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc ggctgtaccg
     1921 ggcggaagac agaacggaac gtctggtgct ggttgcatgc ctgctggccg tgattccggc
     1981 acttaacctt gcctccagtg atgcggcggc tgtagctggt cttgtgatga cggtgctgac
     2041 cgttggtctg gtgtcctgtg cagggaaatc attaccccgt ttctggcccg gagatttttt
     2101 cccgacgttc tatggctgtc atctggctgt gctgggcatt ctggcgttgt gactggtatg
     2161 atactgacgc tgattctgct tattaataaa caacataaca caggatattt atgaaaatta
     2221 cggatcacaa actctctgaa ggtattgccc tgacatttcg tgttcctgaa ggaaatataa
     2281 aacatccttt gattatctta tgccatgggt tttgcggtat caggaatgtt ctgctccctt
     2341 gctttgcgaa tgccttcaca gaagcaggat tcgcaacgat tacttttgat tatcgaggat
     2401 tcggtgagag tgatggagaa cgagggcgtc tggttcctgc aatgcagacc gaagatatta
     2461 tttcagttat caattgggca gaaaagcagg aatgtattga taatcagcgt attgggttat
     2521 ggggaacatc gctgggaggg gggcatgtct tcagtgcagc tgcgcaggac caacgcgtaa
     2581 aatgtattgt cagccagctg gcttttgctg acggtgatgt tctggttacc ggagagatga
     2641 atgaatcgga gagagcgtct tttctatcca cgctgaataa aatggcagag aagaagaaaa
     2701 acaccgggaa agagatgttt gtgggggtta cacgggtact aagtgatgat gaatcgaagg
     2761 tattcttcga gaaaatcaaa gcccggcatc ccgaaatgga tataaaaata cctttcctta
     2821 cggtcatgga aacgttgcaa tataaaccgg cagagagtgc agccagcgtc cagtgcccag
     2881 ttttggttgt tattgccggc caggatagcg ttaatccacc ggaacaggga agagcattat
     2941 atgatgctgt tgcttctggt actaaggaat tatacgagga agctgatgca tgccattatg
     3001 atatttatga gggcgcgttt tttgaacgtg tagtagctgt gcagacacaa tggtttaaac
     3061 aatatcttta attgtcagta tttatacgtt aaaggtcgtt gaaattaact tcctgttacg
     3121 atgagccgtt tcaacggcct gcagggcata ttttcacgaa ggtcagagta agcatggcag
     3181 agcaaaaacg accggtactg acactgaagc ggaaaacgga aggagagacg cctgtccgca
     3241 gccggaaaac catcatcaat gtcaccacgc caccaaaatg gaaggtgaaa aagcagaagc
     3301 tggccgagaa agcagcccgg gaagcggagc tggcggcaaa aaaagcgcag gccagacagg
     3361 cgctgtccat ttatctgaac ctgcccacgc aggatgaggc cgtgaacacc ctgaaaccct
     3421 ggtggccggg attatttgac ggtgacacgc cccggcttct ggcctgcggt atccgggacg
     3481 tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaag ctgcgcaggg
     3541 cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc ggtgcctgcc
     3601 ggtatgacac ggaagggtat gtgacggagc atatttctca ggaggaggaa gcgtatgcgg
     3661 cagcgcgtct ggataaaatc cgccgccaga accggataaa ggcagaactt caggccgtgc
     3721 ttgatgagaa ataaaaaaag cctccctcag agagaggagg cagggaaata aggcttaaaa
     3781 ggaaataact tccgtacccg aaaggaagcc aattcagtag attatttaac accataatac
     3841 tgacgggtta aagaggagtg ataaagatga atggattcag aaacagttca cggaacggtc
     3901 aggtctggcg ttaccagcgt gccggaagcc gggccgttat tctggaagtc agtggacgct
     3961 ggatggaagc ggcagaagca tggcgacggg ctgccggtgt tgcaccccgg actgactggc
     4021 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgagga agggggtaac
     4081 gaaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca tgagatttgc
     4141 tttttttaat atccggtcgg gcagtatgac cggccagttc tgagtagcac agagtttttt
     4201 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tgttattaag
     4261 tgtgtgctgc aatattcagg atgcctgaaa tacaggtgct atttatctga tacggagaat
     4321 aacatgagga aatatattcc actggtatta tttatctttt catggccggt attgtgtgca
     4381 gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca
     4441 cggaaggcgg tgcggatcag gctggtaaat attgatgcgc cggaaaagaa acaggattat
     4501 ggccggtggt cgacagacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca
     4561 tattttcagc gagatcgtta tggtcgtatg ctcgggcagg tttatgcacc agacggaatg
     4621 aatgttaatc agtttatggt ccgggccggg gccgcctggg tatatgagca gtacaacaca
     4681 gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca
     4741 gatgctgatc cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt
     4801 atcattcatc tgttctcagc gggaataaaa atggaaaaaa caaaacagga atggctgtat
     4861 caattacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac
     4921 agtttatcga ccagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcagaa
     4981 ttaatcaccg gaaagttata tgacaggata ccaaaagaaa tatggaaata tgtaaggtaa
     5041 tagttcagca taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct
     5101 cattgttctg attattgcag cgttacttat ccggattatc ctcagaagtg taaatcagca
     5161 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     5221 actgtccggc ggtgaatatg atgccagcca tttacgacct gaacgcagac gccgggatat
     5281 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
     5341 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgctc agcagtcacc
     5401 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
     5461 gcaagttttc cccgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
     5521 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa
     5581 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttaag
     5641 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa
     5701 cgagcataca gaaaggggaa tccgctttct gatgcagaga aacaaagatt atcagtggcc
     5761 cgtaaaagag cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta taaggccatg
     5821 ctcatgcaaa tgtgtcatga agatggcctg actcaggctg aagttctgac cgcactgata
     5881 aaaagtgaag cgcaaaaacg atgtgtgtga tgatgggctt acattcttga gtgttcagaa
     5941 gattagtgct agattactga tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg
     6001 cagggaactg gttctgatgt ggatttacag gagccagaaa agcaaaaacc ccgataatct
     6061 tcagaaagtt tggcgactga gaagattacc ggggcccact taaaccgtat agccaacaat
     6121 tcagctatgc ggggagtata gttatatgct cggaaaagtt caagacttct ttctgtgctc
     6181 gctccttctg cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc
     6241 gccaggtaaa gaacccgaat ccggtgttca caccccgtga aggtgccgga acgctgaagt
     6301 tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc
     6361 atgtggcaca tgcccgttcg aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc
     6421 gggctattga tgcgctgctg caggggctgt gttttcacta tgacccgctg gccaaccgtg
     6481 tccagtgctc catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg
     6541 gaaaactctc catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga
     6601 ttacctacca gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt
     6661 tcacaccggc tctgtttgct gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc
     6721 gcagccgtgt tgaatgggaa aacaggctgc gcaaaaagca ggggctggat accctgggta
     6781 tggatgaact gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga
     6841 cagagcttaa gtcacgggga ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac
     6901 gtcaggatat cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct
     6961 tcactgctaa tcgtgaggcg gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga
     7021 ttctgtcacg taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc
     7081 ctcagaataa tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa
     7141 aaaatcagca ccacatccca aaaacaacct catcatctac cttctggtgc atccggtccc
     7201 ccctgttttg atacaaaaca cgtctcacag acgggcaatt ttgtttatcc acattaaact
     7261 gcaagggatt tctctgtaag attacaaccg ttcatgtcat aaagcaccag ccgccaggct
     7321 tacagggtgc aatgtatctt ttaaacaccc gtttatatct cctttaaact acttaattac
     7381 attcatttaa aaaaaagact tattcactgc ctgtcctgtg gacagacaga tatgcacctc
     7441 ccaccgcaag cggcgggccc caaccggagc cactttagtt acaacacaca aaaacaacct
     7501 ccagaaaaac cccggtcccg cgcagaactg aaaccacaaa gcccctccct cataactgaa
     7561 aaacggcccc gccccggccc aaagggccgg aacagagtcg cttttaatta tgaatgttgt
     7621 aactaagcat catcatcgct gtcagtcttc tggctgaacg ttctcagtac acgctcgtaa
     7681 gcggccctga gggctcgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg
//
LOCUS       DAZFTN010000177         7712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFTN010000177 DAZFTN010000000
VERSION     DAZFTN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(159..302)
                     /gene="ypaB"
                     /locus_tag="ACHKV2_004676"
     CDS             complement(159..302)
                     /gene="ypaB"
                     /locus_tag="ACHKV2_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO6942417.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            430..2715
                     /gene="nrdA"
                     /locus_tag="ACHKV2_004677"
     CDS             430..2715
                     /gene="nrdA"
                     /locus_tag="ACHKV2_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6942418.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2942..4072
                     /gene="nrdB"
                     /locus_tag="ACHKV2_004678"
     CDS             2942..4072
                     /gene="nrdB"
                     /locus_tag="ACHKV2_004678"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6942419.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4072..4326
                     /gene="yfaE"
                     /locus_tag="ACHKV2_004679"
     CDS             4072..4326
                     /gene="yfaE"
                     /locus_tag="ACHKV2_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO6942420.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4380..5030)
                     /gene="inaA"
                     /locus_tag="ACHKV2_004680"
     CDS             complement(4380..5030)
                     /gene="inaA"
                     /locus_tag="ACHKV2_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO6942421.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5111..6301)
                     /locus_tag="ACHKV2_004681"
     CDS             complement(5111..6301)
                     /locus_tag="ACHKV2_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6942422.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6453..7331
                     /locus_tag="ACHKV2_004682"
     CDS             6453..7331
                     /locus_tag="ACHKV2_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6942423.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2059 a         2000 c         1820 g         1833 t
ORIGIN      
        1 aaccatttcg acatcaacaa tcgcttacat tcgcttatat attgaccaca actgatacat
       61 cagattatgt gatgactcgt gcttagatca atttttgcaa tcattagcaa aaagattaat
      121 aaaccatcta tatcaattta tccaacctat tctgccgttc aagaaatcgc cgaacagtta
      181 tttataacaa atttttctct tcccattgac tttcccggac accttgtctg acctaaggtg
      241 cgcgaaagcc actttttcct tcctgagtta tccacaaagt tatgcacttg caagagggcc
      301 attttcacac tatcttgcag tgaatcccaa acataccccc tatatatagt gttctaagca
      361 gcttcccgta ctacaggtag tctgcacgaa actattgcgg aaagaattcc aaaaacaggt
      421 acgacataca tgaatcagaa tctgctggtg acaaagcgcg acggtagcac agagcgcatc
      481 aatctcgaca aaatccatcg cgttctggat tgggcggcag aaggactgca taacgtttcg
      541 atttcccagg tcgagctgcg ctcccacatt cagttttatg acggtatcaa gacctctgac
      601 atccacgaaa ccattatcaa ggctgccgca gacctgatct cccgtgatgc gccggattat
      661 cagtatctcg ccgcgcgcct ggcgatcttc cacctgcgta aaaaagccta cggccagttt
      721 gagccgcctg cgctgtacga ccacgtggtg aaaatggtcg agatgggcaa atacgataat
      781 catctgctgg aagactacac ggaagaagag ttcaagcaga tggacacgtt tatcgatcac
      841 gaccgtgata tgaccttctc ttatgctgcc gttaagcaac tggaaggcaa atatctggta
      901 cagaaccgtg tgaccggcga aatctacgaa agcgcccagt tcctttatat tctggttgct
      961 gcgtgcctgt tctcgaacta cccgcgtgaa acgcgcctgc aatatgtgaa gcgtttttac
     1021 gacgcggttt ccacatttaa aatttcgctg ccgacgccaa tcatgtccgg cgtgcgtacc
     1081 ccgactcgtc agttcagctc ctgcgtactg atcgagtgcg gcgacagcct ggattccatc
     1141 aacgccacct ccagcgcgat tgtgaaatat gtttcccagc gtgccgggat cggcatcaac
     1201 gccgggcgta ttcgtgcgct gggtagcccg attcgcggtg gcgaagcgtt ccataccggc
     1261 tgcattccgt tctacaaaca tttccagact gcggtgaaat cctgctctca gggcggcgtg
     1321 cgcggcggtg cggcaacgct gttctacccg atgtggcatc tggaagtgga aagcctgctg
     1381 gtgttgaaaa acaaccgtgg tgtggaaggc aaccgcgtgc gtcatatgga ctacggggta
     1441 caaatcaaca aactgatgta tacccgcctg ctgaaaggtg aagatatcac cctgttcagc
     1501 ccgtccgacg taccggggct gtacgacgca ttcttcgccg atcaggaaga gtttgaacgc
     1561 ctgtatacca aatatgagaa agacgacagc atccgcaagc agcgtgtgaa agccgttgag
     1621 ctgttctcgc tgatgatgca ggaacgtgcg tctaccggtc gtatctatat tcagaacgtt
     1681 gaccactgca atacccatag cccgtttgat ccggccatcg cgccagtgcg tcagtctaac
     1741 ctgtgcctgg agatagccct gccgaccaaa ccgctgaacg acgtcaacga cgagaacggt
     1801 gaaatcgcgc tgtgtacgct gtctgctttc aacctgggcg caattaatag cctggatgaa
     1861 ctggaagagc tggcaattct ggcggttcgt gcacttgacg cgctgctgga ttatcaggat
     1921 tacccgatcc cggccgccaa acgtggagcg atgggtcgtc gtacgctggg tattggtgtg
     1981 atcaacttcg cttactacct ggcgaagcat ggtaaacgct actccgacgg cagcgccaac
     2041 aacctgacgc ataaaacctt cgaagcgatt cagtattacc tgctgaaagc gtctaatgag
     2101 ctggcgaaag agcaaggcgc gtgcccgtgg tttaacgaaa ccacttacgc gaaagggatc
     2161 ctgccgatcg atacctataa gaaagatctg gatgccatcg ctaatgaacc gctgcattac
     2221 gactgggaag ctctgcgtga gtcaatcaaa acacacggcc tgcgtaactc cacgctttct
     2281 gctctgatgc cgtccgagac ttcttcgcag atctctaacg ccactaacgg tattgaaccg
     2341 ccgcgcggtt acgtcagcat caaagcgtcg aaagacggta ttctgcgcca ggtggtgccg
     2401 gactacgagc atctgcacga cgcctatgag ctgctgtggg aaatgccggg taacgatggc
     2461 tacctgcaac tggtgggtat catgcagaaa tttatcgatc agtcgatctc tgctaacacc
     2521 aactacgatc cgtcacgctt cccgtcagga aaagtgccga tgcagcagtt gctgaaagac
     2581 ctgctcaccg cctacaaatt cggggtcaaa acgctgtatt atcagaacac ccgtgacggc
     2641 gctgaagacg cgcaggacga tctggtgccg tcaatccagg acgatggctg cgaaagcggc
     2701 gcatgtaaga tctgatattg agatgccgga tgcggcgtaa acgccttatc cggcctacgg
     2761 ctcggtttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtga
     2821 acgcctaatc cggcctacgg ctcggatttg taggcctgat aagacgcgcc agcgtcgcat
     2881 caggcacagg atgcggcgta aaatgcctta tccggcatta aactcccaac aggacacact
     2941 catggcatat accacctttt cacagacgaa aaatgatcag ctcaaagaac cgatgttctt
     3001 tggtcagccg gtcaacgtgg cccgttacga tcagcaaaaa tatgacatct tcgaaaagct
     3061 gatcgaaaag cagctctctt tcttctggcg tccggaagaa gttgacgttt ctcgtgaccg
     3121 tatcgattac caggcgctgc ctgaacacga aaaacacatc tttatcagca acctgaaata
     3181 tcagacgctg ctggactcca ttcagggtcg tagcccgaac gtggcactgc tgccgctgat
     3241 ctcgatcccg gaactggaaa cctgggtaga aacctgggcg ttctcagaaa cgattcattc
     3301 ccgttcctat actcatatca ttcgtaatat cgttaacgat ccgtctgttg tgtttgacga
     3361 tatcgtcacc aacgagcaga tccagaaacg tgcggaaggg atctccagct actacgatga
     3421 gctgatcgaa atgaccagct actggcacct gctgggcgaa ggcacccaca ctgttaacgg
     3481 taaaaccgtg accgttagcc tgcgcgagct gaagaaaaaa ctgtatctct gcctgatgag
     3541 cgttaacgcg ctggaagcga tccgcttcta cgtcagcttt gcttgttcct ttgcctttgc
     3601 agaacgcgaa ctgatggaag gtaacgccaa aattattcgc ctgattgccc gcgacgaagc
     3661 cctgcacctg accggtactc agcatatgct gaatctgctg cgcagcggtg cggatgatcc
     3721 ggagatggcg gaaattgccg aagaatgtaa gcaggagtgc tatgacctgt ttgtccaggc
     3781 ggcacagcaa gagaaagact gggcggatta tctgttccgt gacggttcga tgattggtct
     3841 gaataaagac attctctgcc agtacgttga atacatcacc aatatccgca tgcaggcagt
     3901 cggtttggat ctgccgttcc agacgcgctc caacccgatc ccgtggatca acacctggct
     3961 ggtgtctgat aacgtgcagg ttgctccgca ggaagtggaa gtcagttctt atctggtcgg
     4021 gcagattgac tcggaagtgg acaccgacga tttgagtaac ttccagctct gatggctcgc
     4081 gttaccctgc gcatcactgg cacacaactg ctgtgccagg atgaacaccc ttcccttctg
     4141 gcggcgctgg aatcccacaa tgtggcggtt gagtaccagt gccgcgaagg ttactgcggc
     4201 tcctgtcgca cacgcctggt tgcaggccag gtggactgga ttgccgaacc gttagccttt
     4261 attcagccgg gggaaatttt gccctgttgt tgccgagcaa aaggcgatat tgaaatcgag
     4321 ttgtgatcgg gtactagcgc cagatacgac gcttacgcgt attatctggc ctacaacgat
     4381 tacatggcgt agtaatacgc ttttacctgc tcccagtccg ctttggggat cggctccaga
     4441 tatttttcca gctggcggaa gtcatgatta atcgctttat cgcgacgcaa gcggcgacga
     4501 cttttctcca gatcaagaaa accagcttct gccttacctt ctgttttcac atagatatgg
     4561 cgaacataac aacagccatg ctgacgatta acgctatgca ttttcttaaa cgccagcgcc
     4621 actgctttca acatggcttg ccgtacttcg tcagaatagg gcgttactgc atgctgggca
     4681 taccagtcag caatgctgat gaaccccgcc atatcttcag tcaccagcaa cgcgcgccat
     4741 tccccctcaa ttttcaccgc ttcaccaaaa acgatcttcg gcacgatgac accggcccgt
     4801 tccagttctt taattaccgc aacctcacgg acaatcgttg gtcggccgaa aggataacgt
     4861 acggaatgaa acagatgatg cgtcatgcgc tttacatata gctttttgcc gttgcgctcg
     4921 acgcattgca ccccgctcat accattacgg cgatagttag gctcttcaac ccagtcgccc
     4981 tctgttgccc accagtgatt aaattcgtcg tgctttgctg aaactgccat accctatcgc
     5041 ctgtcatttt ttattaacga caatgactat aggtggttac ctgcgaaaaa tcttaatgaa
     5101 acgtgtcgta ttaatgaatt tcttttgcca ctactagcgc gacaccgctt tttcccttca
     5161 tttgcagcgc atagactcca ccagccacta ccactaatag tgcgcccagc agataaatca
     5221 ttcggtagcc agtatgtgca accatcatcc ccatcacaca ggctcccagc gccaggcctg
     5281 catccatcgc attgaagaag agggagtttg cgacaccgat tttatgcggt tccactgaac
     5341 tgataatctg cgtctgaaaa actggcgtca cggaaccata cccgatacca atcaacgcgc
     5401 cagaaacgac catcattacg ctgccctggg tgtagccgag aaccaccagc ccaaccgtaa
     5461 agaacagcag gcaggggtaa accacatatt tcgggccttt tttgtcgcaa acattgccgg
     5521 tgaaggtacg gcagatcatc aggaagatgg cgtagcagag caggaaatta ctggccgccg
     5581 acatcagatt aagttcgcgg gcgtaaagcg ccagaaaggc agaaaccccg gcatacgaaa
     5641 aggtcataaa gaatgtaacc atggcaaacg gcaatgccgc gcgatcgaac atggcggcga
     5701 agcccatctt tggctttgaa ccgtcggcat ggctgataac cggcggcacg gtcataatca
     5761 gcgacaggat aatccccacc gccgcaacgc cagtacataa ccagaaagcc atattaaagg
     5821 cctcccagcg cgccatattc aggccaatcc acggcccgac caccattgcc agccccattg
     5881 ccagagagaa aaaactgatg ccctccccac gtcgggaggc cgggatcagc cgcgcggaaa
     5941 tcgttccctt taccgttgtg ataaccccga aggtaatacc atgaagcacc ctaataaaaa
     6001 gcagtgattc tattgagtgg caaaaaggat acagcgcagt gaccaccaaa aaggccagag
     6061 aagagagcac cagaatagtt ttattcgaat atttacccac ccattgcccg gcaaagggtc
     6121 gaataacaat cgccgcaatt aaaaataaag tcaccaacaa acctgcttta tcaggagagg
     6181 catgcaagcg gtcagagata taaattggca acaatgttag taaaacataa aagacgaaga
     6241 aaataataaa gctgatgatg gttattgccc aaaaatcctt cgtccataac ttctctttca
     6301 tactctaccg cctgtcagga tgacgcacgc agacaacact cccgttactg cggttgcgtc
     6361 aaattgtgat gtactttgca gaacagaagt ataaatggcg gcatgctatt agagaaatta
     6421 atcgttttaa tattattgat tagcaaactc taatgactct tacccaaata aattctttac
     6481 tcgccgtgct ggattacggt ggctttactg aagccagcaa gcgattgttc atgacgcagt
     6541 cggcggtaag tcaggcaatt gctgccctcg aacaagaact tggtgtgaat atactgttac
     6601 gcgatcgccg taaagctata caactgacca cagcaggtca ccgcattgtg caacatctgc
     6661 gcgccattaa tcgggaagtt aacgcagtaa aagagatagc cgagcaggaa aagcagaacc
     6721 ctcaaagaac gctgagactt ggttgtttcc ccagcgtctg cgcctgtatc ttaccggttg
     6781 tgattcgcta ctttgaaatt catcatccga atataaaaat tattcctttt gaggaaaata
     6841 gcacagcgat tattgattcg cttcagaatg aatccattga tgccggtttt gttcattttc
     6901 cggtgagcgg aatgtactcc gtgcccattt atcaggataa attcaccgta gtgttaccgc
     6961 ccaaccatgc tctggcaaaa aacagcacta ttacagtgga agaattaatg ggtgaaccat
     7021 taatcattag taaaggacgc tatgaattaa gcattatggc actatttaaa gaaaagaata
     7081 ttaccccaca gataaaatat gaatttaatc acccggatac agcgatcagt tttattcgac
     7141 agggattagg catcgcctta ttaccagagt taacgctgaa aaccattgca gatgaattgt
     7201 gttccgttcc tctcgaacca accttctatc gacagatttc gttgctggct aaagaaaagc
     7261 cggtagaagg cagtccactg tttttactac aaatgtgtac ggaacaacta gtggtgagtg
     7321 gaaaaatttg atgtaagaca tgagattgat gacaaatgca agactgcctg attcgccaca
     7381 cttagttgta ctatagcctt taccaggcgt aataattcgt tttcacctga ttttggccca
     7441 ctttgtgaat tggcgatata tttatccagt tgggagaagt gacaatgact ccaggtagaa
     7501 accggtatga agcgcccgac tcactgtcaa aaccgattgt tgagagtaat gacactccgg
     7561 cgaacagtac acagtttact ctggagccat tctcaatctt caagtgggat acctaataac
     7621 ttgtcaggga agttatcaat atctgtttgt tacttattac cgtatttatt aacaataatg
     7681 atactttcta acatgcgtaa aggtcttcgt aa
//
LOCUS       DAZFTN010000178         7683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFTN010000178 DAZFTN010000000
VERSION     DAZFTN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..534
                     /gene="alaE"
                     /locus_tag="ACHKV2_004683"
     CDS             85..534
                     /gene="alaE"
                     /locus_tag="ACHKV2_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO6942424.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(571..915)
                     /locus_tag="ACHKV2_004684"
     CDS             complement(571..915)
                     /locus_tag="ACHKV2_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO6942425.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1067..1396
                     /locus_tag="ACHKV2_004685"
     CDS             1067..1396
                     /locus_tag="ACHKV2_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO6942426.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1546..2880)
                     /locus_tag="ACHKV2_004686"
     CDS             complement(1546..2880)
                     /locus_tag="ACHKV2_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6942427.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            2968..3399
                     /locus_tag="ACHKV2_004687"
     CDS             2968..3399
                     /locus_tag="ACHKV2_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6942428.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="ACHKV2_004688"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="ACHKV2_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO6942429.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="ACHKV2_004689"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="ACHKV2_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO6942430.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="ACHKV2_004690"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="ACHKV2_004690"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6942431.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="ACHKV2_004691"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="ACHKV2_004691"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6942432.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
BASE COUNT         1905 a         1971 c         1910 g         1897 t
ORIGIN      
        1 ctatgcgaaa atgcagatgg caatgagatc cactgctttc atctccatta acatcccatt
       61 acgcttttat taaggagcat tagcatgttc tcaccgcagt cacgcttgcg tcatgcagtt
      121 gcagatacgt tcgcgatggt tgtttactgt tctgtcgtga acatgtgtat tgaagttttc
      181 ctctccggaa tgagcttcga acagtctttt tattccagat tggtagcgat tccggtgaac
      241 atcttaattg catggccata cggtatgtac cgcgatctgt ttatgcgcgc ggcacgcaaa
      301 gttagcccgt cgggctggat aaaaaatctg gcggatatcc tggcttatgt gacgttccag
      361 tcaccggtgt atgtggcgat cttgttagtg gtgggcgcag actggcatca gattatggcg
      421 gcggtcagtt caaacatcgt tgtttcgatg ttgatggggg cggtttatgg ctacttcctc
      481 gattattgcc gccgactgtt taaagtcagc cgttaccagc aggtaaaagc ctgactcttc
      541 ttttcgcgac tggcatcgcc agtcgcgtct ttaactggct tcgccaaata aaccattcaa
      601 ataacgttca agcgcaacac gcgaactaaa gccatgcggg ataccgtaat gctcgtgtcg
      661 ctcgcctgct aaatagagcg gaaattgctg gtaatgatcg caagttttaa tatcggaagc
      721 gggttcagcc agtgtcgaac tgcgttcttt taatgttgga tgaattacca gagcggtacg
      781 tcccattcgc gcttcgcgat taacatagac ataattttcc ccacggcggt aaccatacgc
      841 tttctgggtt accacatcga cagtaaaacc gactttttca agtacgcgcg ccacctcgtc
      901 tggtcgtaaa tacatatttg atcctcgtta ttatcaaccg cgggcctacc ttacctgatt
      961 gcgcattcac aacgctttca gaaaagtcca taaagccgcg atgaacctca gttaagaaaa
     1021 ttatggtcta cactgaaaat tacatcgaat tctaatgggg gatcatatgt ttaaccgacc
     1081 gaaccgcaac gacgttgatg atggcgtgca ggatatccag aatgatgtca atcaattagc
     1141 tgacagcctc gaatctgtat tgaagtcctg gggcagcgac gccaaagggg aagctgaagc
     1201 cgcacgcagt aaagctcagg cattgctgaa agaaaccagg gcgcgaatgc atggtcgtac
     1261 tcgcgtccag caagccgcgc gcgatgccgt aggctgcgcg gattcttttg ttcgtgaaag
     1321 accctggtgt agtgtgggaa ctgctgctgc ggtaggtatt tttatcggtg cgttgttgag
     1381 catgcgtaaa tcgtagtgag acgatgacaa gcagtagtct gacttctgaa aagtactcag
     1441 tgcgtcaacg ccttgtccgc ctacaggcct gaaaaacata gcgcatcagg ggattttgcc
     1501 ctggtcacca gtcttatagg accccgaagc ctggcttcgg ggttattaat ttaaagcact
     1561 gcgcaattgc tgtgctacct gtcgcgccat actttcgcta acgatattcg taaacgagag
     1621 caataatcct ccgtcctcgc ttgagcggat acgccagtcg ctaagtgcct gtacggcaag
     1681 accagcagca ttggctttat gcacgaatgt cacgtcatta cctgctagtt tgatcaccat
     1741 ctggataccg cctttttgcg gcacaacctg aaatccctgc tgcgtaagtg cctgctcaat
     1801 ccactgccgt cgctgggcat aatgctggcg cattttcttc agatgccgcc agaaatgccc
     1861 ctcacggatg aaatctgcca gtgtgttctg ccacagtacg ggtacagcac atggttccag
     1921 tgacgcctgc tgccggaatt gcgaaatctg ctccaccggc accaccagcc acgcacagcg
     1981 caatgcagga aatagcgctt tgctgaatgt tccggcataa atcacccgct gcggcgcgtc
     2041 gagacttttt agtgccggta acggcttacc gtgatagcga aactcactgt cgtaatcatc
     2101 ttcaataatc catgcctgac tacgctctgc ccattccagt atctgatacc tacgcgctaa
     2161 agagagtgcc acacccagcg ggctttggtg tgccggagta atcagggcaa aacgcgcatc
     2221 aggataattt tgtattccgc ttgtgacatc cagtccgttg tcatcaaccg gcacaggtaa
     2281 aatttccaca ccgtggcgag tgacaatcgg gcgaatcagc ggaaagccgg gatcttccat
     2341 ccacatcccg tctcccggtt gcgccagagc gtgcagaatt aaagccattg aggccgcata
     2401 accgtgagtg ataaagacct gctctggctg acaatcaatc ccacgtgaaa cgcgtaaata
     2461 atcaacaata gcctcgcgca aggccgcctc gccgcagaca tcgcctaatg ccaaatcaaa
     2521 gcgcgtctgg gtacgcagac ggcggcccat cacccgcgcc cacaattcac gcggaaaaag
     2581 atcaagtgca ggtagcccca tctgaaaagg cagcggctgc tgactttcac cagcaaaagc
     2641 cacgacatcc acagcagact gttgcggatg tatctgctca ctgacaaagg tacccgcctg
     2701 tccccgcctg accagccatc cctgcgccac cagctcagca taagcatttt cgacagtgga
     2761 tcgagagaca cccagctcct gcgaccaggt acggctcgaa ggtagccttg cgccaggttt
     2821 cagttttcct tgctcgataa ccgtttttaa ctgacgggcg atatcctgat aacgtggcat
     2881 atggtctgct tttttaggta atttatggct ctctttagca gtccattata gtgctacatt
     2941 gcgggcgtta ataagaggag aaactgtatg actacattac gtcagcctta ctacgaactt
     3001 agcccggcag tgtataacgc actggtgcag gccaaaacgg cactggaaaa tagcacgctg
     3061 gataccacgt tgatggagtt aatttatttg cgcgtctcgc aaatcaacgg ctgcgcattt
     3121 tgtctggaga tgcacagcaa agcattgcgc aaatccggtg tgccacaaca caaactggac
     3181 gccctggcag gctggcgcgt aagccatcat tttgatgaac gcgagcgagc ggcgctggca
     3241 tgggcggaat ctgtgaccga tattgccaga acccatgcgg aagacgaggt ttatcagcct
     3301 ttgcttgagc atttcagcgc agcagaaatc agcgacttaa cgtttgccat cgggctgatg
     3361 aattgtttta accgtctggc cgtttccatg cggatgtaat ttgctgttgc cgggtatgcg
     3421 cccggcaaaa aaacacaaaa tcccctaccc cgttgagcca ttcaccggtg gcgtaatcaa
     3481 tctaaaatta ccatatcttg tgtggttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcattacta tttacactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcggcagaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt cgggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcagaaaac gatggattac cacgcgctga atgcgatgct taacctctac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttatggcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgacag
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcaac tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgctggaag acgcattttc ctatgccaac caactcggcg
     4981 ctcgtcaggg ggctggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgcttggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccttatga cgtagagcga gtttatggca agccgtttgc cgatgtggcc atcagccaac
     5221 actatgacga actggttgcc gatgaacgca ttcgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacggtaaa ccgtgctaac cctgtcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ctcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 attttgcccg cacggtagag actgccgtgc gcggtttaac ggcagtatca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacacggc tatctggcgc gagaaggcat cgcttatggt tcgccggaag
     5701 cactggattt caccaatctc tatttctatg ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc acgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata ttttagccaa tatctgcaag ggaactggca gccgaaaacg gggaaagttg
     5881 gcgaactgtt tgcccgtagc ggtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatttaca accagaatct tcaggcggtg ccgccaacag
     6001 gttctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 agaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccggctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcctgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cactcacgcc tcatgaagaa gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcgccgttgc
     6781 agcgaaaagc acagattatt cagcaacatt accgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacgcc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaatatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcgac
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattttgtgg tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac cacaatattc aggcgttttt tcgctatctt
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg ggaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccatc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt tccattgggt aatatatcga cataaacaaa
     7681 taa
//
LOCUS       DAZFTN010000179         7629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFTN010000179 DAZFTN010000000
VERSION     DAZFTN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..4844)
                     /locus_tag="ACHKV2_004692"
     CDS             complement(<1..4844)
                     /locus_tag="ACHKV2_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6942433.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFGGID
                     INDFR"
     gene            complement(4864..5325)
                     /locus_tag="ACHKV2_004693"
     CDS             complement(4864..5325)
                     /locus_tag="ACHKV2_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO6942434.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5353..7254)
                     /locus_tag="ACHKV2_004694"
     CDS             complement(5353..7254)
                     /locus_tag="ACHKV2_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6942435.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1478 a         2279 c         2013 g         1859 t
ORIGIN      
        1 cgaaagtcgt taatatcaat cccaccaaac acatctgaat atatatccac tgctgatggt
       61 tttgttttat taagtgttat atcaaatcct tcaataactg gatgtaatcc atattggttt
      121 gtaggagatg tgatatcaat aataccggca tctagacgtt tggaaccacc tgtataatga
      181 gtgccggatg tccacttacc atttacttta aatggtccat tcgttgtcgc aggaactcgc
      241 tctccatttt ttatggctgg ttgaatctca actctaaatt tactacctgt taattctagt
      301 cttttattta tcatgtttat gtatttattg aatgcagcct ctgattttat ccaatcacca
      361 ttagcatatt taagtgctct attccctaat gagttcaaaa catcagatat gttgctttgc
      421 tcactccccc atttcgtcca gcgggctaac tgacatatag ccagccccaa aggatcgata
      481 tacccacaag gattcggcac ataccgataa gtctgctccc ctccctgcaa ccccagcgga
      541 tcactcacca ggtacatccc ggtttccggc tcgtagtacc ggaaccggtt atagcacagc
      601 cccgattctg catcctgcca ctgtcccgca tacagcaggc cggggtccgc ttccgggtcc
      661 cgctccccgc gcgggtccgg gtagtctgtg tctgcgggca ggctgagtgc cagcccccac
      721 aggctggtct gccccggccg ccagacggtt ttaccttcac tgttaaagag catcagcggg
      781 cggcccgtca ggtcgctcac cgcatgattc gttcgcgtca cccactgcgg ggccacggac
      841 ggatgcggct gtcgtacccg gctgaactgc tggcttatca gctcaaagcc gttaaacacc
      901 aggtgcctta cgctgtacag tttatcatcg cggtattccc ggatttccgc aatactgtcg
      961 ccgtcccaca ggtacgtaaa acggattttc ttccggtcgc agcgtttttc cgtccgtctg
     1021 ccgctggcgt cgtggcggta ttcccactgc tccccctgtg cgctgcgata cccggtcagc
     1081 tggtcccggc tgtcccaccg gaaccgctct gtttctgggc ggtagccgtc acggtgtttt
     1141 gtgcggctga ccatccggcc tgcggcgtca tagtcatact gtgtgtttcc ggcctgtttc
     1201 agccggtggc ctgcggcata ctggtcagtc tctccgctta tccggtgtct gcccgcagac
     1261 tgcgccttca ggtagccgca gctgtcatag ccataagtct cgtcgccttc gccatagccc
     1321 agcccggtgc ctgaggctgt caccgccgtg acctggccgt tccggtcaag ccggtactcc
     1381 cggtgtgttt cccgtccgta atcttcccgg ctggtgacgc ctgatacgtt acccgcgctg
     1441 tcgtaacggt attcccgtag cagtgaaggg agcagtccgc cccatgtggc ttccgccggg
     1501 aaaagtccgg cgcgctggct gacaggctga ccggctgcat tccagccctg ttcacaggca
     1561 aaaccccggt tactgtgccg gagcacttca cgtcccgcct catcacggct gaacgtcagc
     1621 gtctcatccc ccggcaggat aacctccgtc agttctccgg cggcgttgta gcggtatgtg
     1681 gttttcagca gggatccgtc ggggtgtctg tcgcgttcat cttccggtgg caggatacag
     1741 cggatgacgg tgtgttcgtc ggggtagtgg tattcaatgg cccgtcggcc ctgagtggtg
     1801 acattcaccc gcccgcgttc atcatagccg gtgcgctgtg atgtgccgtc cgggctgaag
     1861 atacccgtca gcctgccgga tttgtcatac gcatactgcg ttttgccttc cggagtgatg
     1921 acttctgcca ggcggcctgc ggcatcccgg ctgtagagga tggtttcctc caggccgttg
     1981 accctggcgg tacacaggcc atcagcgtca taacggtaac gacagaccac gccgccccag
     2041 tcacgttctt ccgtcacccg gccacagttg tccagaatat aaagataaga ctcgccctgc
     2101 gggttaatga cttctgtcag ttgcagcgtg tctttatcgt agcggtactg ccagcggtgg
     2161 ccttcaccgt ccgttcgcgc caccggcaaa tcaaacgggc cgtactccat tgtccagaca
     2221 acgccgttac cgtccgtgta cgccagcagg ttgccgtggc ggtcgtagcg gaatccttcc
     2281 gcgccggttc ctgcccgctc aaggccactc agtttatggt gctgctgcca ctgcaaccgc
     2341 actgactgac cgccggggcc gcgcatcttc accggcaggt cgtgaccgct gtattcggtg
     2401 tggtgttcgc ggcccatcac gtcggtgagt gtttccggcc agttgtggtg gtcgtactct
     2461 atgcccagac gccgttcatc ctggcggaat atccccgaca gcagcccctg aggggtgtac
     2521 gccagccggg tctcttcgcc ctgtggcgat accagactca ccggcaggcc gcgttcatca
     2581 cactgctgcg tccacgtctg cccggcaggg gcggtgatgt tgctgagcag gccgcgctca
     2641 tcatagcagt actgccaatc cgcgccgtct gccgccgtgt aacggcttaa ctgaccgtgt
     2701 gcggcgttgt attcaaactt ctccacccgc ccggcagggg agtggcgggc cagcaggtga
     2761 tggaattcat cccattcata cgtggtggtg ctgccgtccg gcgcttcttc ccgcagaata
     2821 ttgccgtcag gatcatagta ataacggtga atgcccttgc cgtccgtcac ggtggtgcag
     2881 ccgtcaccat agtccagcgt ggcgttgtaa tagccctccg ccccggtgac ggtcacgcac
     2941 cggccctgtg catcgtaggt gaaacggctc cacgtctggt cgttatcgga ccagcggatg
     3001 atccggtccg cagcgtcgta ctcataaaac aggtggtagt ccagccgcgc atcctgttcg
     3061 taggtcgcca gcgtctgtat gccgttatcc gtgcggtgaa tggctttcag gtagccgctg
     3121 gcgtggtacg tcagtttaag ccggatcccg tcggtgtgac tgacacccgt gatccggtac
     3181 tgggtgtcgc gctcaaacgt gatgcggttg ctggcggtgt cgctcatccc gcccagcatg
     3241 tacatccggt ctgcggggct gtgtgccagg cggtcatcgc tggtatcgtc agtcaccggg
     3301 tggcgcagac gcattcccgg aaaggcgtca taaaagaagc ggctgctcag tgcatccctg
     3361 tcgaacagct ccagataatc atcacggcgg cgcagaatgt actgggcgtg atacgggtta
     3421 accgccgtat cactttcacc gtcgaaggcg aagttcagcg tggctcccag gctgatgaca
     3481 tccacccggt ttccctgttc acgcacccag gcgtattcgc tccaggagtc gctccagccc
     3541 acgcccgtca ggccatgcag cggcagtaca gaactgtgga aacgggtgaa ggagaggggg
     3601 agtgtctggc ccagcgtaaa gtcggtacgt tcgtcgcagt atgcgccggt taccggatcg
     3661 acaggatctg ttacccatct gatgacttta ttggcaatac tttttaacga attacctgtt
     3721 ttccccagca acgcgccgag ctttgcgccc agcagcttac cgcttttccc cagcaggttt
     3781 cccagtttgc ccagtttcgc cagattcccc agttttccca gcagtcctgc tggcggcaga
     3841 agatagctgg ctatcgttat tcctcccagg atcattctct gccatggcgg gaactccggc
     3901 tggatatcca gataggtgac ctgctcaccc ccaataaaga cgtccggcga accttccacg
     3961 attgccgcgc tgcactccag tttgtcgccc ttgcgcgcgg caggctgatt gttgataaag
     4021 acgttgctgc tgccctgggc tatcctcggt ggtttgttct ccttgtcgca gagcgccgtg
     4081 tcctcaccgg cttccgggaa atccacatct tttttgattt cgccacgccc gcccaccatg
     4141 gtggcaaact ccattaatac cggaccggcg ggaaacagaa gcgatgcgcc ggcgctgacg
     4201 cgatccataa aaccggcatt gctgtccgtc agggtgataa cagcgttccc ccaggccttc
     4261 gccagtggta ctgctttttc cgctgccgat tcaagcaggt cagtggcaat atcaccccat
     4321 gagggttccc cctgtgggac ttcaggtatt gctccaggtg ggggaggagg gggaagggtg
     4381 acggctgcac gggctgctgg ctttccgtta atttttacat ttttagaacc ggtattgatg
     4441 gcgccgtccg tgtcacaaaa gctgtttatc catgattcaa gctggttctg tttctcctcc
     4501 acataatcac tcgccttata gctggcatac atcagcgcag gcaccagaac cagcgcataa
     4561 gggccggtgg cacaggcggc cataatcagg caaccttctg ctatggtcac tatcgccccc
     4621 agcacggccc ctgccagcgc ccccaaaaaa ctcttgtgct gaatttcatc gcccagcctg
     4681 gcggcggggg ggccagactt ttttgatgcc aacatctgtg caatggcttt gtcgttggct
     4741 gcccgtttca tctggctggc ggccatcgcc cgctgcgaca gcatccgcat tgccagcgta
     4801 ccgcctgcgg tcgctccctg tggcccgcct ggtccttcac tcatgtttta tccctcgttt
     4861 atgtcagcgc ggctggtcct tttggatgtc attctccggt ggcaacgggg tgaagctctt
     4921 tatcacctcc agcagcatcg tcttttgact gttcttcagg gataaggagg tgctgaaagt
     4981 gaaggtcagc agcgtatctc cacgccgctg gaatacggct atctggctaa tccgcccctg
     5041 attcccttca tcttccggac cgttccagga ataatccagc agccaggccg gggcctctgc
     5101 cagagtgatt tcccggcagg catgttcggt gtacccatca agtttgccct ggtacagcgc
     5161 cagcgtttcc tggtacagct ctggtgtcag ggttccctgt gaaacgggtt cgcgggtaat
     5221 gaccaggttg atgccactgt tatccgtgga taccaggacc tgcatcgact gatcctgcca
     5281 ttgcgatggc agttgaatat gaccttcatt aaaagtgaac tgcataaaat actccctgtt
     5341 aatctgagtg aatcagttga tgaaaacaga accgcccacg atggtgatgc catccttcga
     5401 cagcctgatt accgttgatt ccccgacctg tagctgaatg ccatccggcg tcagcgcaat
     5461 tttgctttcc tccacggcca gcataatttc cttaattgcc gtataagcct gtataccgtt
     5521 gccttcgcca ccattgttta cagcgtcaac atgcaccgaa ccggcaatcg tgcctctgtc
     5581 ctgaaagaca tcctcagata aagacccttt cagtactccg gtgatcttgt ggcctttttc
     5641 caccgtaaga aggcggttac cttcctggat cgtgaccgtc tggtcatttt ttacggtttc
     5701 gcgatgatcg tgttttacgg tggtggtacg gtcgttgagc acttcggtat ccatgttctt
     5761 ctgcgcgtgg atgtagacct gttctctccc tgtcgcatca tcaaacttca gctcattaaa
     5821 cccgctgccc atatacgttt tggaacggat ggtcatctgc gtcttcgtcc ccggcaggct
     5881 gccgggggtg cggttttcct ggtggtaggt gcgccccata atgatcggct ggtccggatc
     5941 gccgttgagg aagtccacta tcacctcctg acccacacgc ggtatcgcca ggttgccaaa
     6001 accggtgcct gcccacgcct gtgccacacg gatccagcac gagctgtcct ggtctgccgg
     6061 gttataacgg tcccagttga atttcacccg cactcggcca tgttcatcac agaagatttc
     6121 ctcaccctcc ggccccgtca cgacggcgct ctgcggaccg tccaccagcg gttttagccg
     6181 cggctggggt cgccatgtcc ggtctgccgg gatcaccgca aaatggttct ccagcgccgt
     6241 ccctgctccc cgccttcccg gcaccgcctg cggctgctcg ccgtgcagtt cacttgccac
     6301 cacctgccat tcccggttca ggttcgcctg cggatgcccc gtcagcacaa ttcgccgtcc
     6361 cggccatatc tccggcgagc gactcattcc ccgcgcggtt tctgcattgt ttcgccagcc
     6421 ctccatctgc cagcgggcaa agttctgccc gtgggcgccc ttgaaacgtc cggggtagtc
     6481 atacacctca tactgcgtgc gctggtaatc ctggtgctgg ccttcctgat caaaacgtcc
     6541 tgcccagccg gggcgtttaa aggtgtagtc tttggtcacc acggaagaag ggcggatttg
     6601 tgcgctgtag cgaaactggc tgatgcagag ggtgctcacc tcggtacggg tgttcgggtt
     6661 ccaggggatt tcaaaagatt cgggcagatg gcggaccgtg tcgcacagca ccaggctctg
     6721 gtcggtactt ttgtaagcat gctcctcata aaagaagatg ccttcctccg ccgccatccg
     6781 gcacaggaaa tcgtaatcag tctcaccgta ctggacacaa aactcacggg aaggatgcgg
     6841 ttcactgaac agcggactcc actcggtcac cccgttttcc tgcaatatcg tgccgaggat
     6901 gcttttgatg tcctcgttct ggaagatacg gaagttctgg cgcagaccgg cacgccacag
     6961 cggcgggtgc accttcatgc tgtacagcat ctggtttttg tcgttctccc ccagttcaaa
     7021 ccaggtcacc acacctttca cccggcgctg tacatcatca ccctgccata ttgtcaggta
     7081 ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc tgaccaagga
     7141 gagatcgagg gaaaagagcg aagagagtga ctgggtgaga tgaaaggaaa ccaccgcaaa
     7201 agcgtccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg acatagcaac
     7261 ctccggtaaa atgaataata atccgcaccg tacaactgcg gataatgaga taagcgggac
     7321 gacttaagac gttattaggg aaggaacaaa aaatgctatg attatgtgag gattttgttt
     7381 aaatattact ttacagaaac gggtgtctgt tcagtcaaaa aaataatcac aagctcaact
     7441 tattgtttcc tgacgttatt caggacaggt gcgcacatca cagcgagtaa caaaaacgca
     7501 aagttaaacc aatattcata atatgttttt attcacaact aaataaccca attaacatca
     7561 ttaaaatgaa tgattgctta aaaaatatat gctaccgctc caccctgata acatgatctg
     7621 cacattgca
//
LOCUS       DAZFTN010000180         7547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFTN010000180 DAZFTN010000000
VERSION     DAZFTN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            151..1779
                     /locus_tag="ACHKV2_004695"
                     /pseudo
     CDS             151..1779
                     /locus_tag="ACHKV2_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
     gene            complement(1779..1901)
                     /locus_tag="ACHKV2_004696"
                     /pseudo
     CDS             complement(1779..1901)
                     /locus_tag="ACHKV2_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2129..3199
                     /gene="dhaK"
                     /locus_tag="ACHKV2_004697"
     CDS             2129..3199
                     /gene="dhaK"
                     /locus_tag="ACHKV2_004697"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO6942436.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3210..3842
                     /gene="dhaL"
                     /locus_tag="ACHKV2_004698"
     CDS             3210..3842
                     /gene="dhaL"
                     /locus_tag="ACHKV2_004698"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO6942437.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3853..5271
                     /gene="dhaM"
                     /locus_tag="ACHKV2_004699"
     CDS             3853..5271
                     /gene="dhaM"
                     /locus_tag="ACHKV2_004699"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO6942438.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            5591..7275
                     /gene="treA"
                     /locus_tag="ACHKV2_004700"
                     /pseudo
     CDS             5591..7275
                     /gene="treA"
                     /locus_tag="ACHKV2_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         1996 a         1865 c         1873 g         1813 t
ORIGIN      
        1 gattttgccc ggtaatagag aattattctc agagaagctc atcatgattg caccatacaa
       61 atatcatgat gataagagga tattattcat tgacttaaac aaaatgacaa aatccataga
      121 cccatagcct gggtactata taggagtggt atgggcatca aacaacacaa tgggaatacc
      181 aaagccgatc gtctcgctga attaaaaatc cgttcgccct caattcaact gataaaattt
      241 ggcgctattg gtttgaatgc aattctcttt tcccccctgc tgatagctgc tgatacagga
      301 agtcaatatg gcaccaatat tactattaat gatggtgaca gaattactgg agataccgcc
      361 gatccatcag gaaacctcta tggtgtaatg accccagcag gaaacacgcc tggcaatatc
      421 aacctgggta atgatgtcac cgtcaatgtc aacgacgcct ctggatatgc aaaaggaatc
      481 attattcagg gcaaaaacag ctccctgaca gctaaccgac tcacagtaga tgttgttggt
      541 caaacctctg ccatcggcat taatttaatt ggtgactata cccatgctga cttaggcaca
      601 ggcagcacca ttaagagtaa cgatgacggc atcattattg ggcatagctc aacactaaca
      661 gccactcaat tcaccattga aaactcgaac ggtataggcc taaccatcaa tgactatggc
      721 accagtgtcg atcttggaag cggaagtaaa atcaagaccg atggaagtac aggtgtttat
      781 atcggtggtc tcaacggcaa taacgccaat ggtgctgcgc gttttacggc gacagacctg
      841 acaatcgatg ttcagggcta cagcgccatg gggataaacg tacagaaaaa ctctgttgtc
      901 gatctcggaa caaacagttc cattaaaacc agtggcgata atgcacacgg cctctggagc
      961 tttggccagg ttagcgcgaa tgcactcact gttgatgtaa ctggagccgc ggccaatggc
     1021 gtcgaagttc gtggtggtac aaccactatc ggtgcagata gccatatttc ttccgcgcag
     1081 ggcggtggtc tcgtcaccag tggttcagac gcgacaatca atttttctgg cacggcagcg
     1141 caacgaaaca gcatcttttc cggcggttct tatggtgcct cggcccagac ggcaacggct
     1201 gttatcaaca tgcaaaatac cgatattacg gttgatcgta atggcagtct ggcgctgggt
     1261 ttgtgggcgc tcagcggcgg tagaataacc ggagacagtt tggctatcac cggcgcggca
     1321 ggagccaggg ggatttatgc catgaccaac agccagatcg acctcacgag cgatctggtc
     1381 attgatatga gtacacccga ccagatggcc atcgcaacgc aacatgacga tggttatgcc
     1441 gccagccgca tcaacgcctc gggtcgtatg cttatcaacg gtagcgttct ttccaaaggt
     1501 gggctaatca atctggatat gcaccctggg tcggtttgga caggttcctc cctcagcgat
     1561 aatgtcaatg gcgggaaact ggacgttgca atgaataaca gcgtctggaa cgtaacaagt
     1621 aattctaatc tcgacacgct ggcgctgagc cattcaactg tcgattttgc cagccacggg
     1681 tcaactgccg gcacatttac cacattaaac gtagagaacc tgagcggtaa cagtaccttt
     1741 attatgcgtg ctgatgttgt tggcgagggt aatggcgtta agccctgggc ctgatgtggt
     1801 acgttccatg tctcccgttt catcagctta ttgcatcgct cccaggaggt tgcaattaag
     1861 ggagatatgc cccgaccatc gttgttaaaa gcgccactca tatcccgcat cccttatgac
     1921 aaaaggtgtt ccggctcgca attttaagta ttaatacacg taaaagtttc aatatggaac
     1981 attcattaaa gcatatgttt cactatgaaa cataatttgg gctaattttc ttttcccttg
     2041 cctgatgcac aatggattca tcggcagtca gtggtcgccg tgtcgttgaa catcatccat
     2101 gccctaccgt aattgctgga gcaaaataat gaaaaaattg atcaatgatg tgcaagacgt
     2161 actggacgaa caactggcag gactggcgaa agcgcatcca tcgctgacac tgcatcagga
     2221 tccggtgtat gtcacccgag ctgatgcccc tgttgcagga aaagtcgccc tgctgtcggg
     2281 tggcggcagc ggacacgagc cgatgcactg tggttatatc ggtcagggga tgctttcggg
     2341 ggcctgtccg ggcgaaattt tcacctcacc gacgcccgat aaaatctttg aatgcgccat
     2401 gcaagttgat ggcggcgaag gtgtactgtt gattatcaaa aattacaccg gcgatattct
     2461 taactttgaa acagcgaccg agttactgca cgatagcggc gtaaaagtga ccactgtggt
     2521 cattgatgac gacgttgcag taaaagacag tctttatacc gccgggcggc gcggcgttgc
     2581 caacaccgta ttaattgaaa aactcgtagg cgcagcggcg gagcgtggcg actcactgga
     2641 cgcctgtgcg gaactggggc gtaagttgaa taatcaaggc cactcaatag gtatcgctct
     2701 cggtgcctgt accgttcctg ccgcgggcaa accttctttt accctggcgg ataatgagat
     2761 ggagtttggc gtcggcattc atggtgagcc gggtattgac cgtcgcccct tctcttccct
     2821 tgatcaaacc gtcgatgaaa tgttcgacac cctgctggaa aatggctcat accatcgcac
     2881 tttgcgtttc tgggattatc aacaaggcag ttggcaggaa gaaccacaaa ccaaacaacc
     2941 gctccagtct ggcgacgggg tgattgcgct ggttaacaat cttggcgcaa ctccactttc
     3001 tgagctgtac ggcgtctata accgcctgac cacacgttgc cagcaagcgg gattgactat
     3061 cgagcgtaat ttaattggcg cgtactgcac ctcactggat atgaccggtt tctcaatcac
     3121 tttactgaaa gttgatgacg aaacgctggc actctgggac gccccggtcc acaccccggc
     3181 ccttaactgg ggtaaataag gagaaagcaa tgtcactgag cagaactcaa attgttaact
     3241 ggctcactcg ttgtggcgat attttcaaca ccgagagcga gtatcttacc ggactggatc
     3301 gcgagattgg cgacgctgac cacgggctaa atatgaaccg aggctttagc aaagtggtgg
     3361 aaaaactccc tgctattgca gataaagata tcggtttcat tctcaagaat accggtatga
     3421 cgctgctttc ctgcgtcggt ggtgccagtg gtccgctgtt cggtaccttc tttatccgcg
     3481 ccgcacaggc gacccaggca cggcaaagcc tgacgctgga agagctttat cagatgttcc
     3541 gcgatggcgc ggacggcgta atcagtcgcg ggaaagccga acctggcgat aaaaccatgt
     3601 gtgatgtgtg ggtgccggtg gtggaatcgt tacgtcagtc cagcgagcaa aatctctctg
     3661 ttccggcggc gctcgaagct gccagtagca tcgccgaatt cgctgcacaa agtacgatta
     3721 cgatgcaagc ccgcaaaggc cgcgccagtt atctcggtga acgcagtatt ggtcaccagg
     3781 atcccggcgc aacctcggtg atgtttatga tgcaaatgtt ggcgttagcc gcaaaagagt
     3841 aaggaattgg tgatggtaaa cctggtcata gtttcacata gcagccgact gggagaaggt
     3901 gtcggtgaat tagcccgtca gatgttaatg agtgataatt gtaaaatcgc cattgctgcg
     3961 ggaattgacg atccacaaaa tcccattggt accgatgccg tcaaagtgat ggaggccatc
     4021 gaatctgttg ctgatgccga ccatgtgctg gtcatgatgg atatgggtag cgcattattg
     4081 agtgctgaaa ctgcgctgga attgctggct cccgagatcg ccgcaaaagt acgtttgtgt
     4141 gctgcgccgt tggtcgaagg tacactggca gcaactgtca gcgcggcctc gggggcggag
     4201 atcgacaaag ttatctttga cgccatgcat gcgctggaag ccaaacgtga acaactgggt
     4261 ttaccgtcct ccgacactga aatctctgac tcatgtcctg cgtacgatga agaagcccgt
     4321 tctctggcgg tggtcattaa aaaccgtaac ggcctgcacg tccgtccggc ctcccggctg
     4381 gtttatacct tatcgacatt taatgccgat atgttgctgg aaaaaaacgg caaatgcgtc
     4441 acaccggaga gtattaacca gattgcgtta ctacaagttc gctataacga tacgctgcgc
     4501 ctgattgcga aagggccaga agctgaagag gcactgatcg ctttccgtca gctggctgaa
     4561 gataactttg gtgaaacgga ggaagttgct ccacctactc tgcgtcccgt tccgcctgtt
     4621 tcgggtaaag ccttttatta tcaaccagtt ttatgtacgg tacaggcaaa atcacccctg
     4681 accgtggaag aagaacagga acgattacgc caggccattg acttcacgtt attagatctg
     4741 atgacgttaa cagcaaaagc agaagccagc gggcttgacg atattgccgc aatcttttct
     4801 ggtcaccata cactgttaga tgatccggaa ctgctggcgg cggcaagcga actccttcag
     4861 cacgaacatt gcacggcaga atatgcctgg cagcaagttc ttaaagaact tagccagcaa
     4921 taccagcaac tggatgatga atatctacaa gctcgctata ttgatgtgga cgatcttctg
     4981 catcgcaccc tggtccacct gacccaaacg aaagaagaac tcccgcagtt taactcgcca
     5041 actattctac tggcggagaa catttatccc tccccagtac tgcaactgga tccggcggtt
     5101 gtaaaaggta tctgccttag cgccggaagt ccggtatccc acagcgccct gatcgcccgt
     5161 gaactgggga ttggctggat ttgccagcag ggtgagaaac tgtatgcgat acaaccagaa
     5221 gaaaagctaa cgctggacgt taaaacgcaa cgtttcaacc gtcagggtta agccaccgtt
     5281 cggatggcat cgttctgatg tcatccggca atttactcag cactaatgtt ttttaactac
     5341 ctgttatatt tttcaccaat ttatgatgat ttttcttcgc taatcgcgaa ccaaacgacg
     5401 ctggctggct aataaacaga cgttatttga tcactctatt tcgccgttat ttatcccttt
     5461 aattcccttt tctaaaatgc ctgacagttc gcagaatgag atttcgatca tgcagctagt
     5521 gcgatcctga actaaggttt tctgatactt gaataccgtt ttattcggtt tcgcctaagg
     5581 agaatgattg atgaaatccc ccgcaccttc tcgcccgcaa aaaatggcgt taattccagc
     5641 ctgcatcttt ttgtgtttcg ctgcgctatc ggtgcaggca gaagaaacac cggtaacgcc
     5701 acagccgcct gatattttat tagggccgct gtttaatgat gtgcaaaacg ccaaactttt
     5761 tccggaccaa aaaacctttg ccgatgccgt gccgaacagc gatccgctga tgatcggatg
     5821 cagcaaaacc agagcggatt tgatctgcgc catttcgtta acgtcaattt caccctgccg
     5881 aaagagggcg agaaatatgt tccgccagag ggacagtcac tgcgcgaaca tattgacgga
     5941 ctttggccgg tattaacgcg ttctaccgaa aacaccgaaa aatgggattc tctgttaccg
     6001 ctgccggaac cttatgtcgt gccaggcgga cgttttcgcg aggtatatta ctgggacagt
     6061 tacttcacca tgttaggact tgccgaaagc ggtcactggg ataaagtcgc ggatatggtg
     6121 gccaattttg ctcatgaaat agacacttac ggtcatattc ccaacggcaa ccgcagttac
     6181 tatttaagcc gctcgcagcc gcccttcttt gccctgatgg tagagttact ggcgcagcat
     6241 gaaggcgatg ccgcattgaa gcaatacctg ccgcaaatgc aaaaagaata tgcttactgg
     6301 atggacggtg ttgaaaacct gcaagccgga caacaggaaa aacgcgttgt aaaacttcag
     6361 gatggtaccc ttctcaaccg ctactgggac gatcgcgata cgccacgacc agagtcatgg
     6421 gtggaagata ttgccaccgc taaaagcaat ccgaatcgac ctgccactga aatttaccgc
     6481 gacctgcgct ctgctgctgc gtctggctgg gatttcagct cgcgctggat ggacaatccg
     6541 cagcaactaa ataccttacg caccaccagc atcgtaccgg tcgatctgaa cagcctgatg
     6601 ttcaaaatgg aaaaaatcct tgcccgcgcc agcaaagctg ccggagataa cgcgatggca
     6661 aaccagtacg aaacactggc aaatgcccgt caaaaaggga tcgaaaaata cctgtggaac
     6721 gatcaacaag gctggtatgc cgattacgac ctgaaaagtc ataaagtgcg caatcagtta
     6781 accgcggccg ccctgttccc actgtacgtc aatgcggcag cgaaagatcg cgccaacaaa
     6841 atggcgacgg cgacgaaaac tcatctgctg caacccggcg gcctgaacac cacgtcggtg
     6901 aaaagtgggc aacaatggga tgcgccaaat ggctgggcac cgttacagtg ggtcgcgaca
     6961 gaaggattac aaaactacgg gcaaaaagag gtggcgatgg acattagctg gcacttcctg
     7021 accaatgttc agcacaccta tgaccgggag aaaaagctgg tggaaaaata tgatgtcagc
     7081 accaccggaa cggggggcgg cggtggcgaa tatccattac aggatggctt tggctggacc
     7141 aatggcgtga cgctgaaaat gctggatttg atctgcccga aagagcaacc gtgtgacaat
     7201 gttccggcga cgcgtccact cagtgaatca acaacacagc cggtaaaaca aaaagaggcg
     7261 gaacctacgc cttaactttg ctgtatatcg agcaatgcca ttatgtgcat aaaattggca
     7321 ttgctctttt ttcgccacga aatgcccccc attctacggt ctatgctttt attttcttat
     7381 atcagagaag attttaattt tattttattt tatcaatttg ttaatcagta cacctctatt
     7441 tatatttttc gccactccgc cattctccct gacaattttc ttgtggaaca aagtgttatt
     7501 taagcaacaa gcaaatcatt ctgtctcttt agacaaattt tgacatc
//
LOCUS       DAZFTN010000181         7430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFTN010000181 DAZFTN010000000
VERSION     DAZFTN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..681)
                     /locus_tag="ACHKV2_004701"
     CDS             complement(<1..681)
                     /locus_tag="ACHKV2_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6942439.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSG"
     gene            complement(692..1693)
                     /gene="ltaE"
                     /locus_tag="ACHKV2_004702"
     CDS             complement(692..1693)
                     /gene="ltaE"
                     /locus_tag="ACHKV2_004702"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO6942440.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1730..3448)
                     /gene="poxB"
                     /locus_tag="ACHKV2_004703"
     CDS             complement(1730..3448)
                     /gene="poxB"
                     /locus_tag="ACHKV2_004703"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO6942441.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3581..4549)
                     /gene="hcr"
                     /locus_tag="ACHKV2_004704"
     CDS             complement(3581..4549)
                     /gene="hcr"
                     /locus_tag="ACHKV2_004704"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO6942442.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4561..6213)
                     /gene="hcp"
                     /locus_tag="ACHKV2_004705"
                     /gene_synonym="priS"
     CDS             complement(4561..6213)
                     /gene="hcp"
                     /locus_tag="ACHKV2_004705"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO6942443.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6357..7256)
                     /gene="lysO"
                     /locus_tag="ACHKV2_004706"
     CDS             complement(6357..7256)
                     /gene="lysO"
                     /locus_tag="ACHKV2_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO6942444.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSRMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1789 a         2007 c         1961 g         1673 t
ORIGIN      
        1 accgctcacc gccataaaat gttcaaactg ttgctgataa ctgagcacct ctggtccggc
       61 ggcttcgaag atgcggtgtt cgttggctgg atggtctaac agcgccacca gatagtgcag
      121 caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc cagcgtggcg gcgttaacac
      181 tggcaggttg tagaccatat cgcgcatgac ttcgaacgct gctgaacctg caccaacgat
      241 tattccggca cgcaattcgg tcactggtac attcgcttca cgaagaatgt ccgccgtagc
      301 ctgacgcgcg cgcagatggt ccgactgctc atgtggcggg gcctgcaacg aactgagaaa
      361 gattaattgc ttaactggta cttcacgtag cgcatcgcgg aagttgagag ccacctggcg
      421 ctcctgagcg ataaaatcgc cgccttcgcc catgctatgc accagaaaat agaccgtatc
      481 gatatcctgt aacagggccg gaaggttatc cggccagctg agatcgactt tatggcaact
      541 gacgtttgcc agttgcagct ttgcaagcct gtcgacatga cgtgccgccg ccaggatctg
      601 atgcccttgc tggctgagtg tgcgcaccag atgctgacca atgtagccac tggcaccaag
      661 aactaaaatg cgttgcggca cgtctccctc cttaacgcgc caggaatgcg cgccagtggg
      721 cggcgacttc tgccagttgt tcgcgcgaga cgtcaagatg ggtcaccagg cggacaatcg
      781 gcgaggcgtt aatcagtacg tttctcgctt tcatgtattc gcctaacgcg gcagcatttt
      841 cttcgccgac gcgaacaaac agcatattag tgtcctgacg catcacatcc gcgcctgctt
      901 cacgcagttg ctccgccatc caggcggcgt tgtcgtgatc ttcctgcaac cgtgcgacgt
      961 tatttttcag cgcatacatc ccggcggcag ccagaatgcc ggactggcgc atcccgccac
     1021 ctgtcatttt ccgccagcga atggcacgtt taatgtaatc acgattaccg acgagtaatg
     1081 aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt gaacgaatca caatattgcg
     1141 tgagctcttt cagttcgctg ccgtaagcca ccacggcatt aaagatgcgc gcaccgtcaa
     1201 catgcagcgc cagattgcgc tcgcgggtaa attcccatgc atctttaagg tattcacgcg
     1261 gcaacacttt gccgttgtgg gtgttttcca gactgagtaa tttggtgcgg gcgaaatgga
     1321 tatcgtcggg tttgattttc atcgccactt tatccagcgg tagcgtgccg tcggcagccg
     1381 cgtcgatggg ttgcggttga atactgccca gcaccgccgc gccaccggct tcaaacagat
     1441 agttatgcgc ggcctgaccg acaatatact cttcgccgcg ttcgcagtga ctgagcagag
     1501 cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc ggcttcttta ccggaaagct
     1561 ctgctgcata gtcctgcaga gcattaacgg tagggtcgtc tccgtaaacg tcgtccccaa
     1621 ccggggcggc catcatcgct tcgagcatgg cgcggctcgg tcgggttacg gtatcactgc
     1681 gtaaatcaat catggcatgt ccttattatg acgggaaatg ccaccctttt taccttagcc
     1741 agttcgtttt cgccagttcg atcacttcat caccgcgtcc gctgatgatt gcgcgcagca
     1801 tatacaggct gaaacctttg gcctgttcga gtttgatctg cggtggaatg gctaactctt
     1861 ctttggcgac caccacatcc accaataccg gaccgtcgat ggagaaggcg cgttgcaggg
     1921 cttcatcgat ttcagaggct ttttctacac gaatacccgt aattccgcac gcttcggcaa
     1981 tgcgggcaaa gttggtgtcg tgcagctcag taccgtctgt caggtagcct ccggctttca
     2041 tctccatcgc cacaaagccc agcacgctgt tattaaagac gataattttc accggcagtt
     2101 tcatctgcac taccgagagg aaatcgccca tcaacatgct aaaaccgcca tcgccgcaca
     2161 tggcgaccac ctgacgttcc ggctcggttg cctgcgcgcc cagcgcctgc ggcatggcgt
     2221 tagccatcga accgtggtta aacgaaccta acaggcgacg cttgccgttc attttcagat
     2281 aacgcgccgc ccacaccgtt ggcgtgccga cgtcacaggt aaagatggcg tcatcggcgg
     2341 caaaatgact aatttgctgc gccagatatt gcgggtgaat ggctttctcg ctcggtttag
     2401 ctaaatcgtc cagccctttg cgggcatcgc ggtaatcttc cagcgcttta tccagaaact
     2461 tgcgatcggc tttttcttcc accaatggca gcaatgcacg cagggtcgac ttgatatcgc
     2521 cgaccagcgc catatccacc ttgctgtgtg cgccgatgct ggctgggttg atatcaatct
     2581 gaataatttt ggcatcggtc gggtagaacg cacggtaggg gaactgagta ccgagtagca
     2641 ctaacgtgtc ggcgttcatc atggtatgga aacctgacga gaatccgatt aacccggtca
     2701 ttccaacatc atacggatta tcgtattcga catgctcttt accgcgtagg gcatgaacaa
     2761 taggtgcttt aattttcccg gcaaactcaa ctaactcttt atgcgccccc gcgcagccgc
     2821 tgccacacat cagagcgata ttgctggaat aacgcagcag ttgcgccagt ttgcgtaact
     2881 cttcttcttc cggcgtcacg actggctgtg gcgcatgata ccagtgggta gttgcccctt
     2941 ctggcgcagg ttttaacgcc acgtcgcccg gtaacacgac aacggaaacg ccacggttaa
     3001 gcaccgcttt gcgcatggca atcgccagca cttgtgggat ctgctccggg ctggaaacca
     3061 gctcgcaata gtgactacat tcgcggaata gctcttgtgg gtgggtttcc tggaaatagc
     3121 cgctgccaat ttcgctggaa ggaatatgag cggcaatcgc cagtaccgga acgtgattgc
     3181 ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc ggggccgcac gatccagcac
     3241 agaccgccag ttctcctgaa agttgtgctt cagcgccagc ggcaaacgcc gccacttctt
     3301 catggcgggt ggacatccac tcgatggtgc ccatgcgatt aagactgtcg ctaagaccgt
     3361 tcagagagtc gcccgtgact ccccagatgc gtttcacgcc tgccgattcg agtgttttgg
     3421 cgatataagc tgcaaccgtt tgtttcatgg ttctccatct cctgaatgtg ataacggtaa
     3481 caagtttagt tcatctgacg gagggggaag ggatgggaga gaaaggaggc actaacggtt
     3541 aaatagcccg atgaaaggaa tatcatcggg cataaggcga ttatgcgaga accaaatccc
     3601 cctgcggatg gcaggagcag gccagtacgt aaccttcagc gatttcggcg tcggtcagcg
     3661 tcattgtact gctcaccgta tattcaccgg aaaccacttt cgtcttacag cagccgcaaa
     3721 cacccgcgcg gcaggcggcg acaaccggaa cgttattgct ttccagcgcc tccagcagcg
     3781 tggtgccaac aggagcgtaa aattcttgcg ccggttgcag tttggtgaat ttcagaccgc
     3841 tggtcgcagc ttccgctact ggggtgaaga atttctcttt aaagaaacgc gtcacgccga
     3901 gtgctttcac ttcctgctct acccaatcca tatacggagc cgggccgcag gtcatcacgg
     3961 tacgtgaagc taagtcagga acgcgagtca ggagttcgcg agtgagacga ccagcgataa
     4021 agccttcggt aacgttattt tccgccacca gcgttaccgg atagttacgc cactcatcgg
     4081 cgaaaataac atcctgcggc gtacgcacgt tgtagatcac ccgcacatcg gcctgtggac
     4141 ggttcttcgc cagccagcga cgcatcgaca taatcggcgt gacgccgcag cctgcagcca
     4201 gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa ttcccccatc gcgtctgaaa
     4261 gccagagata atcaccgcgt tttacatcgc gggtcagcca ctgggagccg acaccgtcat
     4321 caatccgccg cacggtcagg gtgatatatt cactcacgcc tggcgtggag gaaatggtgt
     4381 aagcacgcag cgtttccgct gagttacgca cgctgaccag tgcatattgc ccggcgcggt
     4441 atgggtagta atcgtggcaa atcagggaaa tcgtccacac atccggcgtt tcttgcttaa
     4501 tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg catcgtcatc gacaaactcc
     4561 ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg gtgatagaac gcaggccgaa
     4621 tttctcgttc agcaccgcca gcagatctgg tgtcaggaaa ccaggtgcag tcgggccggt
     4681 gacgatattt ttcacgccca gagaaagcag cgtcagcaga atgacaatcg ctttctgttc
     4741 aaaccaggag agcaccagcg acagcggcag atcgttgaca ccgcagccca gtttttctgc
     4801 cagcgtaacc gccagaataa ttgctgagta agcatcgtta cactgaccgg catccaccag
     4861 acgaggcaga ccttcgatat cgccaaactc aagtttgtta aagcgatatt taccacaggc
     4921 gagagtcagg atcaggcagt catctggcac gctggtggcg aaatcggtga agtagtgacg
     4981 ttcgccgcgt gcgccgtcac agccaccaag caggaagata tgacgcagtt tttcacggct
     5041 caccagatca attagcgtat ccgctgcgcc aagcagcgtc tggcggccaa agcccacggt
     5101 gatcaggtgc ggaatttcgc tgtatgggaa gcctgccatt tgttgcgcct gggcaatgac
     5161 cgcagagaaa tcttcgccat ccagatgacg cacgccaggc cagccaacga tgctgcgggt
     5221 ccagatacga tcgtcataag cgcctacggt tgggtcgatg atgcagttcg aggtcatcac
     5281 gatggggcca gggaaacgag cgaactccac ttgctgattc tgccagccgc tgccgtagtt
     5341 accgaccaga tgcttgaatt tacgcagctc cgggtagcca tgcgcaggca gcatttcgcc
     5401 gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc agcaggttgt agagatcttt
     5461 gagatcgtga ccggaaatca ggatgcattt acccgccgtc gctttgacgt tgacctgggt
     5521 tggcgtcggg tgaccgtatt taccggtttc gcctgcatcc agaatgctca tcactttgaa
     5581 gttcatctgg ccgatttcca ttgaacactc aagaagcgcg ttcatatcgg caggccaggt
     5641 ccccagccac gccatgattt tatgatactg ggcataaata tcgttgtcgt attgaccgag
     5701 aacgtgtgcg tgttccatat aggccgccgc acctttcagg ccatacaggc acagcagacg
     5761 caggccgaga atgttttcgc caatcgccgc tttatctttg ttaggggtaa attctgctgc
     5821 ctgacgttgc agttcgccga gatcgtcgct caccagttgc aggtcagcca tcgggttatc
     5881 gacgcgggcg ttggcatcta cagccaggca ttgtgctttc agcgcctcgc gcaaggcaat
     5941 cgcttcacga gcgtagccga caatacgcgg agaatcgaag ttaacgttgg tcagggttga
     6001 gaaaaaggca cgtggcgcga agctgtcgac atcgtggttg atgatgccgt attcacgcgc
     6061 ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg agtaaatcct gaaggtcaga
     6121 agtttccgcc gttttaccac acatcccctg cgcgtatgag cagccgtttc ctgccggagt
     6181 acggatagtt tgttcacatt gcacacaaaa catgatcaca ccttttaaag ttatatttaa
     6241 tatacatgtt taaggttaag acgcttaacg cggggataaa agggattttt catgcaactt
     6301 taagggagat tgatttagcg caattttggc ggcagggatc taccgccaga gaggtattac
     6361 gcggagaaaa aggcgatgag gatcggcact aacaggctaa gaataaaacc gtgaacaatt
     6421 gctgccggga ccatatccag cccgccagta cgttgaagaa cgggcagggt gaaatccatc
     6481 gacgtcgcac cgcataagcc cagtgcggta gagcggctgc ggcgaatcag cccagggatc
     6541 aacataattg caatcagctc acgggccaga tcattaaaaa acgccgcgct cccgattacc
     6601 ggaccaaaag attcggtcaa taaaataccg gaaagagaat accagccgaa accagaggcc
     6661 attgccagcg cggtattgat gggtagatca agaataaagg cgttaattaa accaccaatt
     6721 aatgaactgg caaccaccac cacggcgaca atcattcccc ggcgattaag gacaatctgc
     6781 tttaaggtca tgccattatt gcgcaactga ataccgacga ggaaaagtag cagaattaac
     6841 gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca gtccactcag gccaatggca
     6901 aaaccaatca ctactacgcc gcacagtttt agcgactcaa gcgccatcgc aatacgcgac
     6961 gggagttttt cttgctgatg gtggttgcgc cacggcagac cgcgctccag ccacataagg
     7021 gcggcaatat tacacagtaa aataacggta atactgacgg cagaataatg cagaatcgcc
     7081 aacaggttac tggcgaggtt atcgagaaac gccagactga tacccataaa aaagagaata
     7141 aggtaaacca tcctgcttaa tagctgatta ataactttta acgcagcttg ttggcgaagc
     7201 ggaatgaggt aacccacaat caggggaacc agaatgatta acaacccaga aaacatgaaa
     7261 aaccagccct tgcaaagaaa gaagtcgaaa tgcgcgatga cacactactg aaagcggaag
     7321 gacgagtaaa gttgcaatta aaaggaaatg ttatgcatca gagggggatt tttaattcta
     7381 tatgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
//
LOCUS       DAZFTN010000182         7430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFTN010000182 DAZFTN010000000
VERSION     DAZFTN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..1030
                     /locus_tag="ACHKV2_004707"
     CDS             8..1030
                     /locus_tag="ACHKV2_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO6942445.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
     gene            1030..2010
                     /locus_tag="ACHKV2_004708"
     CDS             1030..2010
                     /locus_tag="ACHKV2_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6942446.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2007..2765
                     /locus_tag="ACHKV2_004709"
     CDS             2007..2765
                     /locus_tag="ACHKV2_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6942447.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2775..3490
                     /locus_tag="ACHKV2_004710"
                     /pseudo
     CDS             2775..3490
                     /locus_tag="ACHKV2_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            3487..4341
                     /gene="modD"
                     /locus_tag="ACHKV2_004711"
     CDS             3487..4341
                     /gene="modD"
                     /locus_tag="ACHKV2_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO6942448.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4367..6337
                     /locus_tag="ACHKV2_004712"
     CDS             4367..6337
                     /locus_tag="ACHKV2_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6942449.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(6387..6641)
                     /locus_tag="ACHKV2_004713"
     CDS             complement(6387..6641)
                     /locus_tag="ACHKV2_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6942450.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6842..>7430
                     /locus_tag="ACHKV2_004714"
     CDS             6842..>7430
                     /locus_tag="ACHKV2_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO6942451.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
BASE COUNT         1797 a         1974 c         1906 g         1753 t
ORIGIN      
        1 ataacttatg tcactaacca gacggaggtt tacacagatt cttgcgtcga cgttgttcct
       61 gcatcatctg ccttcttttg cacaatcagt caaattctgg gcctcactga cgcttcccga
      121 agcccaaaac attacacgca tcgtcagcgc aggtgcgccc gccgatttgc tattgctggc
      181 tgtcgcgcca gaaaaaatgg tcggtttttc ctcttttgat tttgcccgtc aggcattaat
      241 tccattgtca gagcacattc gccagttacc caggctggga cgactcgccg ggcgcgccag
      301 cacactctcg ctggaagggc ttatggcgtt acatcccgat ttggtcgttg attgcggcaa
      361 tacggatgaa acctggatct cccaggcacg gcaggttagc gaacagacac aaataccctg
      421 gttattgctt aacgggaaac tggcgcaatc agcagaacag ttaacaacgc ttggcaaaac
      481 gttaggcgaa gagcatcgcg ccgcagaaca agccaatctc gccagccgct tcgttggtga
      541 agctcaggca ttcgccgcct cacccgccgc taacctccgc ttttatgctg cgcgcggccc
      601 tcgagggctg gagacgggct taaagggttc gttgcatacc gaggcggcgg aattattagg
      661 tttgcacaac gtcgcgcaaa tagccgatcg ccaaggcctg acacaggttt ccatggaaaa
      721 tctcctgcgc tggcagccgg atattattct ggttcaggag gccgttactg cagattttat
      781 tcgtcgtgat cctctctggc atggcgtgaa agccgttgca gaacaacgca tcttattttt
      841 aagtggcttg ccctttggct ggctggatgc cccaccggga atcaaccgtc ttctgggatt
      901 acgcagactt cacgcctggc tggatcccgc catcaatcgc cagtttaaaa gtgacatgca
      961 gcattacgcc caactgttct ggcattgttc actcagtgac gccgactatc aaaaattggt
     1021 ggcgacctaa tgagaatcgt aaacggatgt attttgctgc tggcagctat cagtatcact
     1081 tttgccgccg taagtggggc ttatcatctc gatatgcagc aactgctggc gctaattctt
     1141 cgtcaagaaa atgttcctgt gcaggaacaa attgtcttct ggcaaatccg cgtgccgcgt
     1201 attcttgctg cgctgttact gggcgcggcg ctggcaggag ccggaaccac ctatcaggga
     1261 atgttgcgta atcccttagt ttcaccggat attttaggcg tctccgccgg tgccggactt
     1321 ggtgcctgca cggcaattct gtggggatta tccattgttt acatccagct gtacgcattt
     1381 tgtggcgggc tgatggtggt cgcgggcgtc tggttgatta cgcgtcgggt gacccgccac
     1441 gatccgatct taaccctggt gctggtaggc atcgcgttag gaacgttgtg cggagcgggg
     1501 atatcgctaa ttaaaacgct ggccgatcct tacacccagt taccgtcaat taccttctgg
     1561 ttgctggggg gactgtccac cgtgacctta cgcgatctct gctatgctgc accgatcatt
     1621 ctcataggct ccttgcccct gttttttctc cgctggcgca tgaatttact cacgctttcc
     1681 gacgacgaag cccgttcgct gggactgaac gtgacgcgtc tgcgctttgt attgattgtc
     1741 tgcgccacac taatcaccgc cagtacggta gcaatagcgg gcataattgg ttgggtcggc
     1801 ctggtcgttc cgcacattgc ccgcttgttg accggtcata accatcagca gctgttgccc
     1861 atggcaatgt gtactggcgc gattctgctc ctgcttaccg atacgctggc acgcagcatt
     1921 ggaacaacag aaatcccact tggcattctg accgcttttg ttggcgcacc gtttttcctg
     1981 cttttgttgt tacgcggagg tcgccagtga ctttgcttgc cgtccgtcat gcgtctctgg
     2041 gttactcccg ccatccggtg ctacgcgacg tgtccttcac cctgtcgcaa ggaacaattt
     2101 gttgtctgtt aggagccaat ggttgcggca aaaccacact catgcgctcc attcttggcg
     2161 ttataccgtt attaaaaggc gaggtcttac tggatagcct ccctgtgcag acactcagcc
     2221 atcgccagcg cgcgcaggct attgcctggg tgccgcaggc gcacgacggt atttttgctt
     2281 ttagtgtgct ggatatggtg ttgatgggcc tggcccccac cataggcgct ttttccgttc
     2341 ccggtaagca agaacgcctg aaagccacgg agcaactgga aaagctgggc attttgcatc
     2401 tggcggcacg acgctggaat acgcttagcg gcggtgaacg tcagctggct ttaatcgccc
     2461 gtgcactcgt ccagcaaccg cgcttgttgt tactggatga gcctgcctcc agcctcgatt
     2521 tcggtcatca gatccaacta ctggacacgc tggcacagct caaaaataac ggcatgacaa
     2581 tgttaatgtc gactcatcat ccgctccatg ccaacgccat tgccgacagc attatccagg
     2641 tagagccaga tggacgagta acacaaggct taccgaccga acagttaaca acaaacaaac
     2701 tggctgccct gtatcgggtt tctgccgacc agattcatca tcacctctct gcaattagcc
     2761 actaaggaaa aatcatgctc attgatgata ttgattttgc agacctttat ctgcagcaac
     2821 tcaagctggc ccaccgcaca gaaaaaacgc cggatcactg ggatcaacgg gcggaaaaaa
     2881 tggcggaaaa ttgcgcctcg cctacggaca gctacctgca acaactgatc gctaaaattg
     2941 acctacaagg cgctcagacc ttattcgata tgggctgcgg accgggtacc gtatcgctgg
     3001 cgctggctga caaactcacg acgatttatg gcgttgatta cagtcagggg atgctgaacg
     3061 ttgccgcccg tcgcgccgcc gcgctgaagg cagacaatgt ccacttgatc cagcgcgcct
     3121 gggaagagga ctggagtgac ctgccacgct gcgatattgc cgtggcttcc cgctccactc
     3181 tggtggctga tatgcgccag gcaatgagca aactgaataa tcaggcacgg cttcgcgtct
     3241 atacgactca tctggtcagc acctcgttcg tctctccagc cattcagcgg gcggctggtc
     3301 gggaagtgat cgaagcacct aggaacgttt tgagcagaac gtcagctgga gtcttggcgc
     3361 gcttaacgac gatgagcgtg agcggttata tcgctggtat caacaacaag atgctcgtgc
     3421 cctcgcccct gccagccgcg actgggcgct catctggtgg gatagcgtac cgcaggaggc
     3481 actgcgatga ttttcctttc tcaggcacaa atcgatgcgt tacttctgga agatatccag
     3541 ggcggtgacc tgaccacgag agcattaaat attggacacc agcatggcta tatagagttt
     3601 tttctccgtc agggcggctg cgtcagcggc gtttctgtcg cgtgtaagat gttaactacg
     3661 ctgggattaa ctattgatga cgcggtcagc gacggttcac aagcgaacgc gggtcagcgg
     3721 ctaatccgtg cgcagggtaa tgccgcagca cttcatcagg gatggaaggc aatccagaat
     3781 gtgctggagt ggagttgcgg tgtttctgat tatctcgatc aaatgctggc gttacttcgt
     3841 gaacgttatc ctgatggcaa tatcgcctgc acccgaaaag caattccggg cacccgctta
     3901 ctggcctcgc aggcaattct ggctgccgga ggactgattc atcgtgccgg atgtgcggaa
     3961 accatattat tgtttgccaa ccaccgccat tttcttcatg acaatcagga ctggtcaggc
     4021 gcaatcaatc agttacgtcg ccacgcaccc gagaagaaaa ttgttgttga agccgacacg
     4081 ccgaaagagg caatcgccgc gttacgcgcg caaccagacg tacttcagct cgacaaattt
     4141 agtccgcaac aggcaactga aattgcgcaa atagcgccgt cgctggctcc ccactgcacg
     4201 ctggctctga ccggcggaat taatctgacg acactcaaaa attacctcga ctgcggcatt
     4261 cgcctcttta ttacctccgc gccttattac gcggcaccgg ctgacattaa agtcagtctg
     4321 caacccgcag cctctattta attctttgtt aaacggaatt tattctatga gactcaaaaa
     4381 acattatctc tgcacagcat tatcacttgc ctttacccag caggccgtag cggctcagga
     4441 gagcgactcg ctgaccgtat ggtccagtcc ggtatcatcg acgacgacca ccgttctcga
     4501 tcaacccacc atgaaggccc tggataaaca gaatgtcgct caggcattaa gtgtcgtccc
     4561 cggcgtggtg ctgcaaaagt caggcagccg caacgaagaa caggtaaaag ttcgtggctt
     4621 tgatagtcgg caggtgccgg tctatttcga cggtgtgccc atttatgttc cctatgacgg
     4681 caacctcgat ctggcgcgga ttctgaccaa caatctgggg gcagtcgaag tttccaaagg
     4741 gtattcgtcg ctgcttcagg gacctaatca gatgggcgga gccattaata tcaccaccca
     4801 gaagccaaca aaacctctgg aagcaagtct gggatatcgc cagggatgga gccgtagcca
     4861 ggacaatgcc tatgatatgc atgcttcatt tgccgccagc agcgaactgg ggtatttgca
     4921 agtcagcggt agccagctaa agcaggattt tctcggcctg ccgcatggtg taaataatga
     4981 tattgcaggc aaacacggca agatgattaa ttcatcggct gatgataaac gcggcattgt
     5041 gaagctcgga tttacaccac gtgaaaacga tgaatacaca ctgacttaca ttaagcagga
     5101 tggtgaaaaa gataacccgc catacagcgg aaatagtggt caaaaatcac gctactggca
     5161 gtggccagag tatgacaaag aaagttttta ttatcaggga acgacccaac taaacgatcg
     5221 ttttaccctg aaaagtcggc tgtatcgcga cacctttgaa aatacgctga tgatgtacaa
     5281 ctcgctggct gatttgaaaa ataaaaaagg cagctacagc cattattccg attacagcga
     5341 cggtgccgga ttacaactgg cagccgatgt gcgtgaaaac gatctgctga cgtttgccgt
     5401 taactggaaa gatgacgtac accgggaaaa aggtgcgccg cacgccgctt acgatcgcta
     5461 tgaagatcgt acctggtcgc tcgccagtga atatcaatgg gctgctgccg ataatgtcga
     5521 tgttgtggct ggaatcagct atgactggcg cgatagcgta gaagcgaaaa aacacgagaa
     5581 agatggcagc atcacccatt atgacgacaa caatcagtca gcttttaact ggcaggtgat
     5641 ggggaaatac cactttgtca atgaagacac gctggcgctt tcgtactatg accgcaaacg
     5701 ctttccgacg ctgaaagaac gctataccac atccaaacct gcgtataacc agatagcgat
     5761 tgttaacccg cagctcaagc cggaacgcgc tcgcggggtg gatttaacct ggaatggtgc
     5821 cttcacgcac gactggggat ttgaggtcag cgtttactat aaccgggtga gtgatgccat
     5881 cctctcgcac aatatcgatg ccgataccat tcaaaatcag aacagcggca cggtggatta
     5941 cagcggtctg gatgccggta ttaaggggaa aatcagcaat atactggatg taggattgag
     6001 ctacgccctg atccacgctg acgccaaacg taaagacatc ggcaagataa ccgatctgcc
     6061 aacgcagaca atgaccgcat ggatgactct caaaccgtgg gagccgttaa gcgtaacgct
     6121 gtcggaagag gcgcgttcct ccagctacag taacagtgac ggttcacaaa aagccgccgg
     6181 ttttgcggtg acccacattc gagccgatta caccttaggt catggcttca gcgtcaatgc
     6241 gtcggtcaat aacctgtttg atacccaata tgcctacagt gaagggtttg ttgaagaagg
     6301 tcgcaatttc tgggcaggtg tcgagtatac gttctgatgt gaaagcggat agcggcatct
     6361 gtatcgctat ccgccttatg aaaatcttat tctcttcgca ggaggcggaa tacgcccaga
     6421 acgagaatag ctcccaccac cgccaccagg aagctgtgca aattaaaacc gctgatggag
     6481 ccgccaatgc caaacatggt cgccagccag ccgccgacca ccgcaccgac tatcccgaga
     6541 atacaggtca ggaaaaatcc accaccatca cgccccggca tgattagctt ggcgataatg
     6601 ccggctatca ggccaaaaat aatccaggca ataattccca tgttcaggcc ctcgtttcga
     6661 tgaatcggac gcaagaaatg cttacgtcaa cgctctaagt ataggcaagg gcgggatcga
     6721 tgctgggcgt atgtcggctg atttaagaac aacatgtaaa aaatattcac gcatttgtat
     6781 taagttttgt taactgtgac cgataaacca aagacagttt gtcagtcagg agtttttccg
     6841 cgtgagtcat taccatgagc agttcctgaa acaaaatccg ttagccgtcc tgggcgtgtt
     6901 acgcgatttg cacaaagccg caattccttt gcgtctcagt tggattggcg ggcagctgat
     6961 cagcaaaata ctggtaataa ccccggataa actggtgctg gatttcggca gtcaagccga
     7021 agacaacatc gccgtgctaa aggcacagca cattaccatt accgccgaaa ctcagggtgc
     7081 gaaagtcgag tttactgttg aacaactaca gcagagtgaa tacttgcagc ttccggcatt
     7141 tattaccgta ccgcctccca ccttatggtt tgtacaacga cgccgatatt tccgcatctc
     7201 cgccccactc catccgcctt atttttgcca gaccaaactg gcggataaca gtacgttacg
     7261 tttccgcctg tatgatttgt cgttaggcgg catgggcgca ttactggaaa cagcaaaacc
     7321 tgccgggttg cacgaaggta tgcgcttcgc tcagattgaa gtcaacatgg ggcaatgggg
     7381 tgtttttcac tttgacgccc agttaatctc catcagcgag cgcaaagtga
//
LOCUS       DAZFTN010000183         7377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFTN010000183 DAZFTN010000000
VERSION     DAZFTN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(679..1485)
                     /locus_tag="ACHKV2_004715"
     CDS             complement(679..1485)
                     /locus_tag="ACHKV2_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6942452.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMQESDAVAMLKQLS"
     gene            complement(1486..1791)
                     /gene="ccdB"
                     /locus_tag="ACHKV2_004716"
     CDS             complement(1486..1791)
                     /gene="ccdB"
                     /locus_tag="ACHKV2_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO6942453.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1793..2011)
                     /gene="ccdA"
                     /locus_tag="ACHKV2_004717"
     CDS             complement(1793..2011)
                     /gene="ccdA"
                     /locus_tag="ACHKV2_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO6942454.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2554..3153
                     /locus_tag="ACHKV2_004718"
     CDS             2554..3153
                     /locus_tag="ACHKV2_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942455.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3150..3770
                     /locus_tag="ACHKV2_004719"
     CDS             3150..3770
                     /locus_tag="ACHKV2_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942456.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3767..3967
                     /locus_tag="ACHKV2_004720"
     CDS             3767..3967
                     /locus_tag="ACHKV2_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942457.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4375..5352
                     /locus_tag="ACHKV2_004721"
     CDS             4375..5352
                     /locus_tag="ACHKV2_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO6942458.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5637..6377)
                     /locus_tag="ACHKV2_004722"
     CDS             complement(5637..6377)
                     /locus_tag="ACHKV2_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6942459.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6498..6728)
                     /locus_tag="ACHKV2_004723"
     CDS             complement(6498..6728)
                     /locus_tag="ACHKV2_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO6942460.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
     gene            complement(6897..7181)
                     /locus_tag="ACHKV2_004724"
     CDS             complement(6897..7181)
                     /locus_tag="ACHKV2_004724"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6942461.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGATLQMMKSKHLK"
     gene            complement(7211..>7377)
                     /locus_tag="ACHKV2_004725"
     CDS             complement(7211..>7377)
                     /locus_tag="ACHKV2_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6942462.1"
                     /translation="LSLKIVNGMDSIKLLDKVIIYQQQNEQLYPAQEHLLLQLCMRVT
                     KKLTDILMPP"
BASE COUNT         1886 a         1883 c         1821 g         1787 t
ORIGIN      
        1 gacaattttt tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt
       61 gaaaaaacag cttcttctga gggcaatttg tcacaacacc ttctctagaa ccagcatgga
      121 taaaggccta caaggcgctc taaaaaagaa gatctaaaaa ctataaaaaa ataattataa
      181 aaatatcccc gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa
      241 gcagaatata taagtgctgt tccctggtgc ttcctcgctc actcgagggc ttcgccctgt
      301 cgctcgactg cggcgagcac tactggctgt aaaaggacag accaaacatc atggttctgt
      361 gttcattagg ttgttctgtc cattgctgac ataatccgct ccacttcaac gtaacaccgc
      421 acgaagattt ctattgttcc tgaaggcata ttcaaatcgt tttcgttacc gcttgcaggc
      481 atcatgacag aacactactt cctataaacg ctacacaggc tcctgagatt aataatgcgg
      541 atctctacga taatgggaga ttttcccgac tgtctcgttc gcttctcagt ggataacagc
      601 cagcttctct gtttaacaga caaaaacagc atatccactc agttccacat ttccatataa
      661 aggccaaagc atttattctc aggataattg tttcagcatc gcaaccgcat cagactcctg
      721 catcgcaaac tgcacccggt gccgggcagc cacatccagc gcaaaaacct tcgtgtagac
      781 ttcctttgaa ctgatggact tatgtcccat caggctttgc agaactttca gcggtatacc
      841 agcatacagc atgtgcatcg cataggaatg gcggaacgta tgtggtgtga ccggaacaga
      901 gaacgtcaca ccgtcagcag cagcggcggc aaccgcctcc ccaatccagg tcctgaccgt
      961 tctgtccgtc acttcccaga tccgcgcttt ctctgtcctt cctgtgcgac ggttacgccg
     1021 ctccatgggt attttcagtg ttgccaccat cgtctgcagc tggctgacgt accaggagtc
     1081 agagagcgga accagccggt gagtctgctg accggcgggc attctccccg ccgtcctggc
     1141 agctttttcg gtccgttgtt tcagggtcgc aagctgcaca aacggatacg gaggcgcaag
     1201 cgaaaaatcc ccccgcgtca gcgccagtgc ttcattaatg cgtgctccgg tgttccacag
     1261 tgtggccagc agcatcttgc ggtgcagatt cgggacgtaa tggagcaggg cactcacttc
     1321 cggagccagc agatattttg gcagttcatc atggaccatc gacatctggc gaagtgccag
     1381 agctgccgga taatcaatgg caacaggcag cgatgcaggc tgtccggcag aatacactgc
     1441 cggtaccatg actgcagact ggctgtgtat aacggagcct gacatttata ttccccagaa
     1501 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
     1561 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
     1621 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
     1681 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
     1741 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagt
     1801 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
     1861 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
     1921 ttgtgcttac cagaccggag atattgacat catatgcctt gagcaactga tagctgtcgc
     1981 tgtcaactgt cactgtaata cgctgcttca tagcacacct ctttttgaca tacttcgggt
     2041 atacatatca gtatatattc ttatgccgca aaaatcagcg cgcaaatacg catactatta
     2101 tctggctttt agtaagcctt atgtatttta cctttcgtta tgttaaccaa taaaaattaa
     2161 aatctgcctt ataaaaacaa agcgtaatta ccgcattccc gtttcgtatg gatccgactt
     2221 tatttgaata aacgactttc cggaagtgta ccacctgaga aaatcatcat atcgctgttt
     2281 tccgcccctg cgcggcgcat cacagtccag agatacaaca caacatatag tgccatgggc
     2341 aactgaatga ggcactgcat gctgtgctga cgggatgtaa cgctgtgcgg agggagagga
     2401 agatccgtaa ccaccagaaa ccggatctga ccaggaaaag agaaagagtg tatgtaagtt
     2461 ctgtactgta tgtatgaatt gtattgtatg tattgtgtat ttttcatacc gggcatacga
     2521 tggcgccatt ccctgagaaa ccggagccag accatgacat ccctctcccc ggacaccgtc
     2581 cgccgcattg aagacgccgc cgccgccctg atcgccgccg gcaccccgaa cccgaccaac
     2641 gagcaggtcc gtcagcacct cggcggcggc tccctgtccc acatctcccc ggtgatgcgc
     2701 gcgttccgcg cccgccagcg cgagcaggcc acgcccctgc cgccggagct ggcgcagctg
     2761 ctgaccggcc agctggggct gctgtggcag gcggccgtga aacaggctga agccggggcc
     2821 ctggcggcgc gcgagcaggc cgacgacgac attgcccggg ccgacaagga gcgggatgag
     2881 gcgctggcga acgtggccgc gctggaaagc gagctggcgg tgctgcgcga ggtggtggcc
     2941 gaacgtgacc gcctgctgca ggaggtacgc gagctacgcg cggaggcgct gccgctgcgc
     3001 gagcaggtgg cccgcctgac cgccaccggc gaacacctgg cggcgcagct gcaggacacg
     3061 aaggcggagc tgaaggaagc ccgggaggac ggccgccagc ttcagaccga actgctggct
     3121 ctggcccgcc aggacgggaa ggtgaagaaa tgagacagga aagagtatta cgtataaagg
     3181 catttcgacg ggcactggag cttggtggcg gtgaacgggc tgacgggtgt cgtcattttc
     3241 cccgctggaa aacggaactg aggaatttcc cgcacagcag ctgcgattta gccagcaata
     3301 ctctggcgta ctacctcaaa gaacgagaga gatgccatcc gtgcatcatt tttatggaag
     3361 ggaacgctgg ctttcatgaa gcggaaaact caacagttca cgggcatgtc atcgtattgc
     3421 tggacggaga gtacatcgat ttgacgctgg atcaattcga cgaatatccg gactacattc
     3481 cttacgaacc tgtcgaatct gacgggcaga ttggtaagct gttgcgtaat atcatgaagc
     3541 atgaggaccc ggtaaaaacc cgccagattg atctcgatgg tggcgaagag ctgtatgcct
     3601 ggttacgcga taccgccgac gaagtgctgg cagcagatcc ggaatggcag gcccgggaac
     3661 ggtcgataga agaagcaagg gaagccgcta ttacggtgtt cccgtttctg tctgacgtgc
     3721 aaaaaactga gtgcgaacag ggcccggaca gtcaggaggc tgccagatga agatttatca
     3781 ttacactgac ctgaacggcc tgaagggcat catcgaatca ggctcactgt gggcgacgca
     3841 cttttccttt ctcaatgaca gtaatgagct gactcacggc atgaactgcc ttgagaatgc
     3901 cctgcagtac ctgagggacg actttaaccc gaaaaccctt aagttcatcg aacaggcacc
     3961 cttataagcg gttgtgtatg ctgtttccct ggaatgattt atccacatat ccggaggctc
     4021 gatccagtaa acagatccat gaatgatcaa caaaggatcc attaaagatc cccataccgc
     4081 tgcaaacctt gtcactcatg ggccgggacc acgatcacat aagcagtggc atgttactga
     4141 taaactgtaa catgctaatg ataagctgta ttcagtaatc catatactga agtaagttaa
     4201 tgacataaac tatggtcagt acgccagact cagctgttaa atacaggctg caggtttttc
     4261 ttcagtcagt tagcggggct ctgacacacg atttgctgtt tattctttta ctgtccacag
     4321 gcaggaggct ttctggaaaa cgaaaattca gacatcaaaa aactgttcgg cgaggtggat
     4381 aagtcgtccg gtgagctggt gacactgaca ccaaacaata acaacaccgt acaacctgtg
     4441 gcgctgatgc gtctgggcgt ttttgtaccg acccttaaat cactgaagaa cagtaaaaaa
     4501 aatacactgt cacgtactga tgccacggaa gagctgacac gtctttccct ggcccgtgct
     4561 gagggattcg ataaggttga gatcaccggc ccccgcctgg atatggataa cgatttcaag
     4621 acctgggtgg ggatcattca ttcctttgcc cgccataacg taattggtga caaagttgaa
     4681 ctgccttttg tcgagtttgc aaaactgtgt ggtatacctt caagccagtc atcacgcagg
     4741 ctgcgtgagc gcatcagccc ttccctgaaa cgcattgccg gtaccgtgat ctccttttcc
     4801 cgcaccgatg agaagcacac ccgggaatac atcacccatc tggtacagtc agcctactac
     4861 gatactgaac gggatattgt tcagttacag gctgatcccc gcctttttga actgtaccag
     4921 tttgacagaa aggtccttct ccagcttaag gcgattaatg ccctgaagcg acgggagtcc
     4981 gcccaggcac tctacacctt tatagagagc ctgccccggg atccggcacc ggtatcgctg
     5041 gcgcggctgc gtgcacgcct caatctgaag tctcctgtat tttcccagaa ccagacggtc
     5101 agacgggcaa tggagcagct gcgcgagatt ggatatcttg attacacgga gatccagcgg
     5161 gggcggacaa aactcttctg cattcactac cggcgtcccc ggttaaaagc accgaatgat
     5221 gagagtaagg aaaatccgtt gccaccttca cctgcggaaa aagtcagtcc ggagatggcg
     5281 gagaagcttg ccctgcttga gaaactgggc atcacgctgg atgacctgga aaaactcttc
     5341 aaatcccgct gaacataaac tgtagtcagt gaagagtgtt cctttactga ctacagctta
     5401 tattatcagg tgcagtgagt ggtctgctca ctgcagttta tattcagttt cctgcagtgc
     5461 tgcctgtagc tgagctgtca tctgccggtc ccttacgtga gtcaccccgt aacctgatgc
     5521 tgaggcattg ctcccttcat aaaacatgac ttactcacta cagcttatat acatgctcca
     5581 gcttatgtta tgtctgttct gctgaccaca gcttatataa ggaagcgcgt atgccgtcac
     5641 ctcaggggag gcagtgtacg caggatctcc gcagcatccc gcccgtcacc tgtgaaaggc
     5701 actgccagcg tggcagccat atccagcgca aacactctgg tataaacctc catcgaacgt
     5761 ggatccctgt gacctgccag ggcctggatg actttccggg gctggcggtg atagagcata
     5821 tgcatgatat agctgtgccg gaaggtgtgt ggtgtgaccg gaatcgaaaa gtgtactccg
     5881 tcagcttcgg cccgtctgac agcctgcttc agccagttgc gcatggtttc gtcggtcacg
     5941 gcccataatg gttcacgacg acggggccgg gtggtgatca tccagctttc catctgcctg
     6001 acatagctta tatccgtcag cggaaccagg cggacttcat cttttggcgg gcgtccgcgt
     6061 cgcgcacgca ctttttcgga caggatccgc acaaacggcc ttgctccatc caggtcaaat
     6121 gattccggcg tcagcatccg ggcttcgcca atacgcattc cggtattcca gagggtggcg
     6181 aacagcatat ggtgacgctg atccggcata tagaaaagaa gggcactcac ttccggggcc
     6241 agcaggtagg ccggtgtggc ccctgtggaa gtggccattc ttctcaggga gagcgctgtt
     6301 gcaaaatcca cccccggcgc aatgggtaac aggaagacgc gttctggtga attctgcagg
     6361 ggaatgacat tgttcatgag ttatttttca cgtagcacac gcactgttta tggatacagg
     6421 tattctgacc tgaacgcccg gatccttcca cgacaataaa tcgcagcgtg cggatccttt
     6481 tgtttgctta tcacgcatta ttgcgggtaa aaccaaatac gtaaaggtac gttttgcagc
     6541 agggatcccg tattaagcca gtcgtggcgc gggctgaggg ctggtttgcc gtttttggga
     6601 gaattggggt aatgccagga cgctaatgat ctatcaatgg atggatgtaa gtgacaattg
     6661 aaaactgaaa aatgcgctat gtagattctg acagatttat gcacctgaaa tacgttcaaa
     6721 atccacatag cgtaactctg attttcctta ccagaaaaaa gagggggaat aaccagtatc
     6781 tatcccccct acacaattgc agaataataa attataaaaa taagaactca ggatttatat
     6841 acatgattac atgtaatatt actgagaaat attttgagat aaaacctttt tgctgattat
     6901 tttaaatgtt ttgatttcat catctgtaag gttgctccat atttttttaa atagcgtcca
     6961 tatatccttg tggtccgctg taaatacaaa ttaccgtctg aagtcagata aatattctta
     7021 ctacgtcgat cttccttact ttccatacgt ttaataaaac caaatttttc cagagaatca
     7081 caagctctgg tgatatatgt tctggaaaca tctaatcttt cactgagttc agtcgaagat
     7141 aagcagaaat tatcagtact ggataacaag gctagaagca taagcgttgt atcgtttata
     7201 ccatccccct tcagggaggc attaatatat cagtaagttt tttggtaact cgcatacaaa
     7261 gttgtaaaag aagatgttcc tgtgctggat ataattgctc attctgttgt tgatagataa
     7321 taactttatc aagtaattta atactatcca ttccattaac tatttttaaa gataaaa
//
LOCUS       DAZFTN010000184         7374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFTN010000184 DAZFTN010000000
VERSION     DAZFTN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..546
                     /locus_tag="ACHKV2_004726"
     CDS             <1..546
                     /locus_tag="ACHKV2_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6942463.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..4020
                     /locus_tag="ACHKV2_004727"
     CDS             607..4020
                     /locus_tag="ACHKV2_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6942464.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            4091..4690
                     /locus_tag="ACHKV2_004728"
     CDS             4091..4690
                     /locus_tag="ACHKV2_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6942465.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4750..6066
                     /locus_tag="ACHKV2_004729"
     CDS             4750..6066
                     /locus_tag="ACHKV2_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6942466.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGTDGTLITG
                     LTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            6068..6337
                     /locus_tag="ACHKV2_004730"
     CDS             6068..6337
                     /locus_tag="ACHKV2_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6942467.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            6339..6446
                     /locus_tag="ACHKV2_004731"
                     /pseudo
     CDS             6339..6446
                     /locus_tag="ACHKV2_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6514..7374
                     /gene="nleB2"
                     /locus_tag="ACHKV2_004732"
                     /pseudo
     CDS             6514..7374
                     /gene="nleB2"
                     /locus_tag="ACHKV2_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
BASE COUNT         1892 a         1656 c         2271 g         1555 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacaccggg ctattttcag ataaccgcca cgccgcatct tgcggtttat
     2821 gatccgacgg tacagtttga gttctggttc tcggaaacgc ggattgcgga tatcaggcag
     2881 gttgaaacca gcgcgcgtta tcttggtacg gcgctgtact ggatagccgc cagtatcaat
     2941 atcaaaccgg gccatgatta ttacttttat atccgcagtg tgaacaccgt tggcaaatcg
     3001 gcattcgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct ggattttttc
     3061 aaaggcaaga taaccgaatc tcatctcggc aaggagctgc tggaaaaagt cgatctgacg
     3121 gaggataacg ccagcagact ggatgagttt tcgaaagagt ggaaggacgc taacgataaa
     3181 tggaatgcca tgtggggcgt caaaattgag cagaccaaag acggcaaaca ttatgtcgcg
     3241 ggtattggcc tcagcatgga ggacacggag gaaggcaagc tgagccagtt tctggttgcc
     3301 gccaatcgta tcgcgtttat tgacccggca aacgggaatg aaacgccgat gtttgtggcg
     3361 cagggcaacc agatattcat gaacgacgtg ttcctgaagc gcctgacggc ccccaccatt
     3421 accagcggtg gaaatccacc ggtattttcc ctgacatcag acggaaagct gaccgctaaa
     3481 aatgcggata tcagtggcag tgtgaatgcg aactccggga cgctcaacaa cgtcacggta
     3541 aatgaaaact gtacgattaa gggcatgctg gaggcgactc aggtcagagg tgacttcgtt
     3601 aaagctgtat ccaaatcatt tccgaaacag gctggtacgt ggggtaacac ggaaacacca
     3661 aacgggacgg ttacagtcac catcagcgat gatcataact ttgaccgtca aatcattatt
     3721 ccgcccatta tctttaacgg aatagcgtat agcgatccgg gaagtggtaa taacccggga
     3781 ggtacaagat acacgggtta tggttttgaa gttcgcaaaa acggtgtatt aatcgcatcc
     3841 agagaaacta aaggggccat tcccggtagc tacagtgcgg ttattgatat gccgagtggc
     3901 aggggaagcg tcactctgga gtttaaggtt ttccataaag gcaatcagcg ggcaggtaat
     3961 atcaccgact gtacggtgat tgtgaccaaa aaagcggctt ccggcatcag tatccgttga
     4021 aatatttata accctaataa cgggcgccag aaatggcgcc ttttttattg cagaaaatcg
     4081 ggaggtaatt atgcgtaaag tttgtgcagc aattttgtcc gcagccattt gtctggccgt
     4141 atccggtgtg cctgcatggg cgtctgagca tcagtccacg ctgagcgcag gatatcttca
     4201 tgccagtacg gacgctcccg gcagcgatga tctgaacggg attaacgtga aataccgtta
     4261 tgagtttacg gacacactgg ggctgattac gtccttcagt tatgccaacg ccgaagatga
     4321 gcaaaaaacg cattacagcg atacccgctg gcatgaggat tacgtgcgta accgctggtt
     4381 cagcgtgatg gcagggccgt ctgtgcgtgt gaatgaatgg ttcagtgcgt atgcgatggc
     4441 gggtgtggct tacagccgtg tgtcgacttt ttccggggat tatttccgcg taactgacaa
     4501 caagaggaaa acgcacgacg tgctgaccgg aagtgatgac gctcgctaca gcaacacatc
     4561 tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tcagtggccg ttgatgtcgc
     4621 ttatgaaggc tccggcagcg gtgactggcg cactgacggt ttcatcgtgg gtgtcggtta
     4681 taaattttga ttagtaacac agcgttatga cagcccgccg gttcaggcgg gcttttttgt
     4741 ggagtgggta tggcagcagt acaaatatca ggcgtgctga aagatggtgc gggaaaacca
     4801 atacagaact gcaccattca actgaaagcc agacgtaaca gcaccacggt ggtggtgaac
     4861 acggtggcct ctgaaaatcc ggatgaggca gggcgttaca gcatggacgt cgagtatggt
     4921 cagtacagcg tcactctgtt ggtggaggga ttcccgccat cacatgccgg gaccatctcc
     4981 gtgtatgaag attctcaacc gggtacgctg aatgattttc tcggtgccat gacggaggat
     5041 gatgcccgtc cggaggcact gcgtcgtttt gaacagatgg tggaagaagc agcgcgtcac
     5101 gcagaggagg cgaagaagaa tgccggagaa gcagagacgt ccgcgaggaa tgccggcata
     5161 tcagccagta aggcggaagc gagcgccgca aatgctgata cttcagcaga ggatgcatcg
     5221 gagtcagccc ggcaggcggc agaaagtgca gcctctgcaa agaagtcaga ggaagcgtcc
     5281 tcgtcctcag cctctgaggc cgctcaaaaa gccagtgagt cattacaaag tgcaacagat
     5341 gccgagttgt caaaaaagac ggcagaaagt gcagccggta atgcagccag ggatgcaacg
     5401 accgcaacag aaaaagcccg ggaatcagca gaaagcgcac agtcagcgga acaaagcaga
     5461 atagcggcgg aagacgccgt aaacagaatt cccaccgtgg tggggcctcc cggaccaaag
     5521 ggggaaccgg gtcccgcggg tcctcagggg ccgaagggag ataaaggaga gcgtggagac
     5581 accggtccgg caggggcaac cggtgaacgg ggaccgggag gagatacagg tccggcaggt
     5641 ccgcaggggc cgaaaggcga caggggagag cggggagaga ccggtctgac aggaagtaca
     5701 ggtccacagg ggccaaaggg agataccggg gcaacaggtc cggcaggacc gcagggaccg
     5761 aaaggggaaa caggtgcggc tggcccggtg ggggctaccg gacctcaggg ggcgaagggc
     5821 gacccggggg agacacaaat acggttccgt ctggggccga tgagaattat tgagacaaac
     5881 agctatggct ggttcccggg tacagatggt acgctcatca ccggactgac ctttcttgac
     5941 cccaaagatg ccacacaggt tcaggggatg tttcagcatt tgcaggtcag atttggtgac
     6001 gggccatggc aggatgttaa gggactggat gaagtgggca gtgatacagg cagaactgga
     6061 gaatgacatg aatattttga gaaagcttat gcagagtctg tgcggttgcg gaaagcatga
     6121 tgactgtgaa aacggacggt cacttacagc acaattgcga ctgggaccgg cagacattct
     6181 ggagtcagat gagaatggca ttattccgga gcaggacagg gtaatcacac aggtggtgat
     6241 actggatgca gataaaaagc agatacagtg tgtggtaaga ccgctgcaaa tcctgcgtgc
     6301 tgacgggacg tgggaaaata ttggcgggat gaagtaaccc gacagcttca caaaaccgga
     6361 gtccggctcc ggtttttgtt gtcatgtccg gtggatgttt gttaggaaag caaagatggc
     6421 aaaactgctg gaggttttgt ggttgagtat gccaatataa ttaatagatt aaagagttag
     6481 ttgtgaagaa aatatggata aacaggacga cgaatgcttt caccgataag gacaactttc
     6541 cataactcag taaatatagt gcagagttca ccctgtcaaa cggtttcttt tgcaggaaag
     6601 gaatatgagt taaaggtcat tgatgaaaaa acgcctattc tttttcagtg gtttgaacct
     6661 aatcctgaac gatataagaa agatgaggtt ccaatagtta atactaagca gcatccctat
     6721 ttagataatg tcacaaatgc ggcaaggata gagagtgatc gtatgatagg tatttttgtt
     6781 gatggcgatt tttcagtcaa ccaaaagact gctttttcaa aattggaacg agattttgaa
     6841 aatgtaatga taatctatcg ggaagatgtt gacttcagta tgtatgacag aaaactatca
     6901 gatatttatc atgatattat atgtgaacaa aggttacgaa ctgaagacaa aagagatgaa
     6961 tacttgttga atctgttaga gaaagagctg agggaaattt caaaggcgca ggattctttg
     7021 atttctatgt atgcaaagaa aagaaatcat gcatggtttg atttcttcag aaatttagcc
     7081 ttattaaaag caggagagat attcaggtgc acatataata caaagaatca cggtatttca
     7141 ttcggggagg tgtatctatc ttgatatgga tatgatactt acaggtaagc ttggtacaat
     7201 atatgctcct gatggaattt caatgcatgt ggatcgtcgt aatgatagtg taaatattga
     7261 aaatagtgca ataattgtta accgtagtaa tcatcctgct ctacttgagg gactttcttt
     7321 tatgcatagt aaagtagatg ctcatccata ttatgatggt ttggggaaag gagt
//
LOCUS       DAZFTN010000185         7295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFTN010000185 DAZFTN010000000
VERSION     DAZFTN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..1482
                     /gene="adeQ"
                     /locus_tag="ACHKV2_004733"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             149..1482
                     /gene="adeQ"
                     /locus_tag="ACHKV2_004733"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1535..1987
                     /gene="yicN"
                     /locus_tag="ACHKV2_004734"
     CDS             1535..1987
                     /gene="yicN"
                     /locus_tag="ACHKV2_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO6942468.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2103..2426)
                     /locus_tag="ACHKV2_004735"
     CDS             complement(2103..2426)
                     /locus_tag="ACHKV2_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942469.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2410..2763)
                     /locus_tag="ACHKV2_004736"
     CDS             complement(2410..2763)
                     /locus_tag="ACHKV2_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6942470.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2997..4187
                     /gene="nepI"
                     /locus_tag="ACHKV2_004737"
     CDS             2997..4187
                     /gene="nepI"
                     /locus_tag="ACHKV2_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO6942471.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4228..4521)
                     /gene="yicS"
                     /locus_tag="ACHKV2_004738"
     CDS             complement(4228..4521)
                     /gene="yicS"
                     /locus_tag="ACHKV2_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO6942472.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4743..5561
                     /gene="nlpA"
                     /locus_tag="ACHKV2_004739"
     CDS             4743..5561
                     /gene="nlpA"
                     /locus_tag="ACHKV2_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO6942473.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5565..6488)
                     /gene="yicL"
                     /locus_tag="ACHKV2_004740"
     CDS             complement(5565..6488)
                     /gene="yicL"
                     /locus_tag="ACHKV2_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO6942474.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6599..6772)
                     /locus_tag="ACHKV2_004741"
                     /pseudo
     CDS             complement(6599..6772)
                     /locus_tag="ACHKV2_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6952..7057
                     /locus_tag="ACHKV2_004742"
                     /pseudo
     CDS             6952..7057
                     /locus_tag="ACHKV2_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            7068..7133
                     /locus_tag="ACHKV2_004743"
                     /pseudo
     CDS             7068..7133
                     /locus_tag="ACHKV2_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT         1793 a         1609 c         1819 g         2074 t
ORIGIN      
        1 ttgattgtta tttatcataa agaaatgtcg caagattatt aaagaatatc cattaatgtg
       61 caattgaaat gtgataatta tcactgaacc gttgttgtca tttgttttac aaaagcaagg
      121 gattaaatct cctccaatgg acaaaaaaat gaataatgac aataccgatt acgtgagtaa
      181 tgaatcaggg acgctttcgc gattatttaa actacctcag catgggacca ccgtccgcac
      241 agaattgatt gcggggatga ccactttttt aaccatggtg tacatcgttt ttgtgaaccc
      301 gcaaatcctc ggcgcggcac aaatggaccc gaaagtggtg tttgttacca cctgtttgat
      361 tgccggtatc ggcagtattg cgatggggat atttgctaac ttacccgtgg cgctggctcc
      421 ggcaatgggg ctgaacgcct tctttgcgtt cgtggtcgtg ggggcgatgg gtatctcctg
      481 gcagaccggg atgggcgcga tattctgggg cgcaattggg ctatttttgc tcacgctatt
      541 tcgtatccgg tactggatga tctccaacat tccattaagt ttacgtattg gtatcaccag
      601 cggaatcgga ttatttatcg ccttaatggg attaaaaaat actggcgtta ttgtcgccaa
      661 taaagacact ctggtgatga ttggcgattt aagttctcac ggcgtgttgt taggtatttt
      721 agggtttttt attataaccg tgttgtcatc acgtcatttt catgccgcgg tgcttgtttc
      781 tattgtggtg acgtcttgct gtggattatt tttcggtgat gttcatttta gcggcgtcta
      841 ttccattccg cctgatatta gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac
      901 acttgaactc gccggtatca ttttctcctt tatgctgatc aacctatttg attcatcagg
      961 aacattaatt ggtgtaactg ataaagctgg cttaatagat agtaacggta aattccccaa
     1021 tatgaataag gcgctatatg ttgatagcgt agttcggtgg cgggcgcgtt tatcggcacc
     1081 tcgtctgtta ccgcctatat tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg
     1141 ctgactgcgg ttgtggtcgg cgttatgttc ctgttggtta tgttcttctc accgctggtg
     1201 gcgatggttc ctccttacgc aaccgccgga gcgttaatct ttgttggcgt gttgatgacc
     1261 tcaagtctgg cgcgcgttaa ctgggatgat tttaccgaat cggtgcctgc gtttgttacc
     1321 acggtgatga tgccctttac tttctcgatc accgaaggga ttgcacttgg ctttatgtcg
     1381 tactgcatca tgaaagtgtg caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg
     1441 gtcgcagctc tgtttgcact gaagattatt ctggtggatt agtaactggc gagggtttcc
     1501 agatatcatg agttctgatt acgcaggaga actcatgatc tggataatgc tcgccacgct
     1561 ggcggtagtg tttgtggttg gttttcgggt gctgacatcc ggggccagaa aagcgattcg
     1621 ccgtctcagc gatcggctga atatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat
     1681 gggaaagtca gccggtgacg aatttttacg ttatttgcat cgtccggatg agtcgcacct
     1741 gcaaaacgcc gcgcaggtgt tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca
     1801 gaacctgctg caatggcatc ggattttaca aaaagcccgc cttgccgctc ccattactga
     1861 tgctcaggtc aggctggcgc tgggttttct gcgcgaaacc gaacctgaaa tgcaggatat
     1921 taatgctttt cagatgcgct ataacgcgtt ctttcagcct gccgagggcg ttcactggct
     1981 gcattaaaac cgcttcgtct tatcagccta cagaccgcgc ctgacctgta gggtggataa
     2041 ggcgttcacg ccgcatccgg tatcggaact gacagtaggt gaacgcatta tctacgttct
     2101 atttacagca gcacctccaa cccgtgtttt tgcacaatgc tgagcagttt taatgaaatg
     2161 ccattggggc gtttagcgcc actttcccat ttttgtacag ttgaaacgct ggtattgaga
     2221 tagtgagcga agacgggctg actcactttt aatttttccc gcagtgcttt tatctcaaca
     2281 ggttgtaagt cttgcacgct ggtcaggcag gctttgtcaa aagtacgcat cgtttcagca
     2341 gaaatagcat tgacgctgga caatccagcc gctgcgctgt gaatagcctc aaatgcagga
     2401 cttttaaatt tagtttttgc agtcatagca gatctctgtc agttctttag tgttaatcat
     2461 tgctgttagt tgggctttcg tcaggaaggc ataatgcttt gcaatttcac gaaacccagc
     2521 cagttcctga ctattgatgt tcgacatatc ctgttttgca tataaaaacg tgtaaaacca
     2581 attgcttacc cctttcgcca gaataattgc acgctcacga ttttggtgaa ggcgcttttt
     2641 aaaaacgcca ccgccaagtg catcagcttt accttgttct gtttctaaaa tggcccggca
     2701 caactcttca tctgttattg catgagacct ggcttccctt gcaaaccatt tggttttaaa
     2761 cacgcgtata tcgttgaggt cctgttatta aagtatatca ccaagtgcaa tactttatcc
     2821 cttgttccca acacaatcgc gaaaatacat atcaccttgt cctatgattt ttatttcttc
     2881 gagcagcctc gagcttttcc cacaacaccg taaaagttgc aaaaacgtta aatgcgtcac
     2941 acatttcaat gttaaagtgg tcggcttttc ccctgaaaca tgccacgggt aacaccatga
     3001 gtgaatttat tgccgaaaac cgcggcgcgg atgccatcac ccgaccgaac tggtcagccg
     3061 ttttctcggt ggcgttttgc gtcgcctgtc tgattatcgt tgagtttttg cctgtcagtt
     3121 tgttgacgcc aatggcccag gatttaggca tttcggaagg ggttgccggg caatcggtga
     3181 ccgtgaccgc ctttgtggca atgtttgcca gtttgtttat tacccagaca attcaggcca
     3241 ctgaccgccg ctacgttgtt attttgtttg ccgttttgct gacgctctcc tgcttgctgg
     3301 tttcctttgc taactcattc agtttgcttt taatcggtcg tgcctgtctg gggctggcgc
     3361 tgggcgggtt ctgggcgatg tcggcgtcgc tgaccatgcg tctggtgccg ccgcgtacgg
     3421 tgccgaaggc gctgtcggtg atcttcggcg cggtttctat tgcgctggtg attgccgcgc
     3481 cgttgggctg ttttttaggc gagcttatcg gttggcgcaa tgtctttaat gcggcggcgg
     3541 cgatgggtgt gctgtgtatt ttctggatta tcaaatcact gccttcgctg ccaggtgaac
     3601 cttcgcatca gaaacaaaat actttccgct tattacaacg tccgggagtg atggccggga
     3661 tgatcgccat ctttatgtcc tttgccggac agtttgcttt tttcacttat attcgcccag
     3721 tgtatatgac tctggcggga ttcggtgttg atggcttaac cctggtgctg ctgagttttg
     3781 gtatcgccag ctttgtcggg acatcgcttt cgtcgtttat tctcaaacgt tcggtgaaac
     3841 tggccttagc gggcgcaccg tttgtgctcg ccctgagcgc gctggtactg acgctgtggg
     3901 gaagcgataa aattgttgct actggtgtgg cgattatctg ggggctgacc tttgctttaa
     3961 ttcccgttgg ttggtcaacg tggatcactc gctcattggc cgatcaggca gaaaaggctg
     4021 gatctattca ggttgccgtt attcagcttg ccaatacctg cggtgcggca atcggcggtt
     4081 atgcgttgga taacattggt ctgacttcgc cgctgatgtt gtccggcaca ttgatgttgc
     4141 tgactgcatt gttggtgact gcaaaggtga aaatgaagaa atcctgatat caggtaaaga
     4201 agcgttggtc agtggttgtt gccgttctta catatccggg cattctacct tacccaacgc
     4261 ttcccaataa ttgctctgat tattacgttc catcgccacg atggcctcgc gtatatgttg
     4321 tttattgttt tcatctgaca acattaactt ttcccacgct tcatctgata atgacgcctg
     4381 tggcgtgcaa attttacgta aatcttgcaa taccgtggtt ttctcttttg ccgactgtag
     4441 agaactgaat ggcgactcgg cctgagaaag ggctggaaaa atcaggaata cggtaatcat
     4501 gagtagcatc gttggcttca tagcggcaac cttctgcaaa tgtgcatcga atgaagcata
     4561 agtgtagttt gcttttgccg ctctcatcat gctgtacgaa aggtgtttgc gtggtcagta
     4621 agaagtgcca gactttatat tccactttta ttccttttta ttcttatcga tagcgttttg
     4681 ttttttaaac tgcagcgacc ttaccgctat agtccggtaa tcattaataa aaggataaaa
     4741 aaatgaaact gacaacacat catctacggg cgggggccgc attattactg gcaggagttc
     4801 tgctggcagg ttgcgaccag agcagcagcg atgaaaagca tattaaagtt ggcgttatta
     4861 atggcgcaga acaagatgtc gcagaagtcg ctaaaaaggt agcaaaagag aaatatggcc
     4921 tcgatgttga actggtagga tttagcggtt cgttgctacc gaacgatgcg acaaataatg
     4981 gcgagcttga tgcgaacgtc ttccagcatc gaccctttct tgaacaagat aatcagacgc
     5041 atggttataa actggtagcg gtgggaaata cgtttgtctt ccctatggcg ggctattcca
     5101 aaaaaatcaa aaccgtggcg caaataaaag aaggtgcgac agtggcgatc cccaacgacc
     5161 cgactaacct tggtcgcgcg cttttactgc tgcaaaaaga gaagctaatt actctgaaag
     5221 agggaaaagg gttattacct accgcgttgg atataaccga taatccacgc catttgcaga
     5281 taatggagct ggaaggggca cagttgccac gtgtactgga cgacccgaaa gtggatgtag
     5341 cgattatcag caccacttac atcgagcaaa caggactttc tccggttaac gacagcgtat
     5401 ttattgaaga taaaaattcg ccgtatgtga atattctggt ggcacgggaa gataataaga
     5461 atgcagaaaa cgtgaaggaa tttctgcaat cttatcaatc acccgaagtc gctaaagcgg
     5521 cagaaaccat ctttaacggt ggtgcggtgc ctggctggta attctcactc atgccgcgcc
     5581 ggacgattta tttttctggc gcggcggcgg gaatccattg aaatcaaaat caccgatgac
     5641 agaatcagca gcgttcccag ccagtccggc aatgtgaagg tgatccccag caacagcaaa
     5701 gagagcagcg cgctacttaa tggttcagca cagcttaaaa tgctggcttt tggaccgcca
     5761 attaattgtg ctcctttcag gtacagacta aatgtcaggg acgtaccaat gaccaccaaa
     5821 taaaaaaacg ccagaatcaa acttccgtta acgataaagt ttgtcccttg tccggcataa
     5881 aagggcagca ggatcagacc gccaatgagc atactccagc cgacgaccgg taacgtgcca
     5941 tagcgggcaa tcagcgttga gggataggtg gtatagaatg cagcagtaaa tgccgaggca
     6001 atgccccaga ataacgcggc aggagagatc gataatgacg tcggattacc gtgtgtcacc
     6061 aataaaaaag tcccgatcag cgatgtcaaa atagcgcaga aaaccagaat gcccgggcgg
     6121 gatttacgca ccagtgagaa ccaggcgacg ataatagtcg gtgagaggaa ttgcagcacc
     6181 gttgccgtgg ctgcgttcga tttttcgatg gttagcaaaa aagtgagctg tacggttagc
     6241 gcgccaacca cggaaaaaat cagtaggcta atggcatctt tgtgattgtt aataatagaa
     6301 aagattttat caccatgaac aaatgatagc gtcagcagaa tcaaaccggc gaatatcaaa
     6361 cgcgtcatag tcaaaaactg cgacgacatc tggctttgct ccatgatgta ttgcgcgcag
     6421 acccctgaac ttccccacaa cacggcggca atcagaacgt tcagcatccc ctttctggtg
     6481 gaacccattt ttccctctgc atactgtttt gtcgtttatg tatgcaggca tgatagcaaa
     6541 aatggcgagg atggctcagt ttgcgccacg ccgatttttc ttagcccaga ctggtgagct
     6601 aaatatcttt aataaacagc aagcaaatca ttgcaatacc caacatccct atcgccagcc
     6661 agaacaacgt gtggtagctc cagatttcaa ccataatttc gtcaacagag ccggcgataa
     6721 tctagccgaa gcgtgaggta tttgcatata acgttgttgc ggaacctatt cttatttatc
     6781 ggagtgcctt gccagtaatt tactgacgag gatcctcata atagcgttac ccatctgttg
     6841 cattgagact ttacaggccg ttattctaaa tgaccaaaat taaatcagtg cataagggga
     6901 agtattctgt agtgatgtct ccctgagatg caggttccac tggagaaaat tatgagcagt
     6961 ttgtttcaga atgaaataaa ggataaattt aaaactggac ttgcatatgt gcgggggcca
     7021 gaaaaaagcg gatcttccgc tcaaaatgca ggtagcgaag gtggtaaaaa atgcgccggg
     7081 agaagcagaa gattgtcccg cgatgtccgt ttgagaaaca cgagtttgat taaacgcgtg
     7141 agcatgatga tattagtagc tgaccagaga aattatatcc gtcgtaagca ataaaaatca
     7201 gaagtgatgt tatggatatt tttaacctga ataactccgt aagtacgggg ttattcaggt
     7261 ttttgctagc ctgtgctttt ctataccaga tgtgg
//
LOCUS       DAZFTN010000186         7268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFTN010000186 DAZFTN010000000
VERSION     DAZFTN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..675
                     /gene="ehxC"
                     /locus_tag="ACHKV2_004744"
                     /gene_synonym="hlyC"
     CDS             160..675
                     /gene="ehxC"
                     /locus_tag="ACHKV2_004744"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO6942475.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            677..3673
                     /gene="ehxA"
                     /locus_tag="ACHKV2_004745"
     CDS             677..3673
                     /gene="ehxA"
                     /locus_tag="ACHKV2_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO6942476.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3723..5843
                     /gene="ehxB"
                     /locus_tag="ACHKV2_004746"
                     /gene_synonym="hlyB"
     CDS             3723..5843
                     /gene="ehxB"
                     /locus_tag="ACHKV2_004746"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO6942477.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5847..>7268
                     /gene="ehxD"
                     /locus_tag="ACHKV2_004747"
     CDS             5847..>7268
                     /gene="ehxD"
                     /locus_tag="ACHKV2_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO6942478.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINE"
BASE COUNT         2347 a         1118 c         1625 g         2178 t
ORIGIN      
        1 aggcaatata ttattatcgc taatgttgtt ttggttggga gagattctcc ttgcggtagc
       61 tttctgtttt tagatgcttc ttgcttaaaa ggatataatt tctgttcttt tatatagggt
      121 tctttacatt tactgagttt tcttatttat ttaaacatta tgaaatctaa tgcttttgac
      181 gtcatgggga aggttgcatg gctatgggcc tgttctcctc tgcataaaaa atggccatta
      241 tctgtttttg ctattaatgt catacctgct attcagacaa atcaatttgc tctcttaata
      301 aaggatgagc ttcctgtagc tttttgtagc tgggccagtt tagatctgga atgtgaggta
      361 aaatatataa atgatgtaac atctttgtat gcaaaagact ggatgtcggg agaaagaaag
      421 tggtttattg actggatagc tccatttgga cacaacatgg agttatataa atacatgagg
      481 aaaaagtatc cttatgagtt attcagagca ataaggttag atgagagcag taaaacagga
      541 aaaatagctg aatttcatgg aggggggata gataaaaaac tggcatcaaa aatattcagg
      601 caataccatc atgaacttat gtctgaagta aaaaacagac aagattttaa ttttaatatt
      661 gagaaagaaa actaatatga cagtaaataa aataaagaac attttcaata atgcgacatt
      721 gactacaaaa tcagcattta atacagcatc atcaagcgta cgttccgctg gaaaaaaact
      781 catattatta atacctgata attatgaagc tcagggcgtg ggtattaatg agttggtcaa
      841 agctgctgat gagcttggaa tagaaataca ccgtactgaa cgagatgata cagcgattgc
      901 aaaccagttt tttggtgcag cagaaaaagt tgtaggatta actgaacgtg gtgttgcaat
      961 attcgcacca caacttgaca aacttctgca gaagtatcag aaagttggga gtaaaatagg
     1021 aggaaccgct gaaaatgtag gtaataatct gggaaaagcc ggaacagttc tctcagcact
     1081 acagaatttt acggggattg ctttatcagg catggctctt gatgaattgc tgagaaaaca
     1141 acgggaagga gaggatataa gtcagaatga tattgccaaa agtagtattg aacttattaa
     1201 tcagcttgta gatacagtat caagtataaa cagtaccgtt gattcatttt ctgagcagct
     1261 taaccagctt ggctcatttt tatccagtaa acctcgatta agttctgttg gtgggaaatt
     1321 acaaaattta ccagacctgg gccccctggg ggatgggctg gatgttgtct ccggaattct
     1381 ttctgctgta tcagcaagct ttattctggg aaacagtgac gcacatacag gaacaaaagc
     1441 tgcagcgggt atcgaactga caactcaggt tcttggaaat gttggtaaag ctgtttcgca
     1501 atatattctg gctcagagaa tggcacaggg gttatcgaca acagctgcaa gtgcgggtct
     1561 gatcacatcg gctgttatgc tggctatcag tcctctttct ttcctggctg ctgcagataa
     1621 atttgagcga gctaagcagc ttgaatcata ttctgaacga tttaaaaaat tgaattatga
     1681 aggggatgct ttactcgcag cctttcataa agaaaccgga gctatagatg cagccctgac
     1741 aacaataaat actgtcctga gttctgtatc tgcgggagtt agtgcagcct ccagtgcatc
     1801 cctcataggg gccccgataa gcatgctggt gagtgcatta accggtacga tatctggcat
     1861 tctggaagca tcaaaacagg ctatgtttga gcacgttgca gagaaattcg ctgctcggat
     1921 caatgaatgg gaaaaggagc atggcaaaaa ttattttgag aatggatatg acgcaagaca
     1981 tgctgcgttt ttagaagact ctctgtcttt gcttgctgat ttttctcgtc agcatgcagt
     2041 agaaagagca gtcgcaataa cccagcaaca ttgggatgag aagatcggtg aacttgcagg
     2101 cataacccgt aatgctgatc gcagtcagag tggtaaggca tatattaatt atctggaaaa
     2161 tggagggctt ttagaggctc aaccgaagga gtttacacaa caagtgtttg atcctcaaaa
     2221 agggaccata gacctttcaa caggtaatgt atcaagtgtt ttgacattta taacaccaac
     2281 atttacccca ggagaagaag ttagagaaag aaaacagagt ggtaaatatg aatatatgac
     2341 atctcttatt gtaaatggta aggatacatg gtctgtaaaa ggcataaaaa atcataaagg
     2401 tgtatatgat tattcaaaat tgattcagtt tgttgaaaag aataacaaac actatcaggc
     2461 gagaataatt tctgagctcg gagataaaga cgatgtggtt tattctggag caggctcatc
     2521 agaagtattt gctggtgaag gttatgatac cgtatcttat aataagacgg atgttggtaa
     2581 actaacaatt gatgcaacag gagcatcaaa acctggtgag tatatagttt caaaaaatat
     2641 gtatggtgac gtgaaggtat tgcaggaagt cgttaaggaa caggaggtgt cagtagggaa
     2701 gcgaacagag aaaatacaat atcgtgattt tgaattcaga accggtggaa ttccttatga
     2761 tgtaatagat aatcttcatt ctgttgaaga gctcattggc ggaaaacatg atgatgaatt
     2821 caaaggcggt aagtttaatg atatattcca tggcgcagat gggaacgatt atatcgaagg
     2881 taattatggt aatgatcgac tatacggcga tgatggggat gattatatat ccggaggaca
     2941 gggagacgac cagttatttg gtggtagtgg aaacgataaa ttgagtggag gggatggtaa
     3001 taattatctg acaggaggaa gcggtaatga tgagcttcag gcacacggag cttataatat
     3061 tctgtcaggt ggtactggtg atgataaact ttatggtggt ggtggtattg atcttctgga
     3121 tggaggggaa ggtaatgact atctgaatgg tggttttggt aatgatattt atgtttatgg
     3181 gcaaaactat ggtcatcata caattgcaga tgaaggaggt aaaggagatc gtttgcactt
     3241 atctgatatt agctttgatg atatcgcatt taagagagtt ggaaatgatc ttatcatgaa
     3301 taaagccatt aatggtgtac tttcatttaa tgagtcaaat gatgtcaatg ggataacatt
     3361 taaaaactgg tttgcgaaag atgcctcagg agcagataat catcttgttg aggttataac
     3421 agataaagat ggtcgagaga taaaagttga taagatacct cataataata atgaacggtc
     3481 aggttatata aaagccagta atatagcatc tgaaaaaaac atggttaata tcaccagtgt
     3541 tgccaatgat attaataaga ttatttcttc agtttcaggg ttcgattcag gtgatgaacg
     3601 attagcatct ttatataatt tatccttaca tcaaaacaac acacactcaa caactttaac
     3661 gacaactgtc tgagactaaa agaggaggtg atggccattc caggaggaat ggtcatcgtt
     3721 atatgatgag taaatgtagt tctcataata gtctgtatgc actgatattg cttgcacaat
     3781 atcataatat aactgtcaat gctgaaacta taaggcatca gtataatacc cacacacaag
     3841 attttggggt gactgaatgg ttactggcag cgaaatctat tggcttaaaa gcaaaatatg
     3901 tagaaaaaca tttttccaga ttgtcaataa tttctttacc tgcgttgata tggcgggatg
     3961 acggtaagca ttatatattg tctcgtatta ctaaagattc atcacgctat cttgtttatg
     4021 atccagaaca acatcagtca ctaactttta gtcgggatga gtttgaaaaa ctgtatcagg
     4081 gaaaagtcat tctggttacg tcaagagcaa cagtagtcgg agagttagct aaatttgatt
     4141 tttcttggtt tatcccctct gttgtgaaat acaggaggat tttacttgag gtgttaactg
     4201 tttctgcttt tattcagttt cttgcgttaa taacacctct tttttttcag gttgtaatgg
     4261 ataaggtttt agttcaccgg gggttttcaa cgttaaatat tatcacaata gcatttatta
     4321 tagtgatact ttttgaagtg atattaaccg gagccagaac ttatattttc tctcatacta
     4381 caagtcgtat tgacgtcgaa ctgggtgcta agttattcag acatttgctt gcattgcctg
     4441 tttcatattt tgaaaatcgc agggtcggag agaccgttgc cagagtaagg gaactggagc
     4501 aaattcgtaa ttttttaacc ggacaagcgt tgacatcagt tcttgatcta tttttttctg
     4561 taatattttt ttgtgtcatg tggtattaca gccctcaatt aacactggtt atattattgt
     4621 cactaccttg ttatgttata tggtcattgt ttatatcacc cttattacgt cgacgtcttg
     4681 atgataagtt tctcaggaat gcagaaaatc aagcttttct tgtcgaaacg gtaacagcaa
     4741 taaatacaat caaatccatg gcagtatcac cacagatgat tgctacatgg gataaacaac
     4801 tggccgggta tgttgcttcc agtttcaggg taaatctggt tgcaatgaca gggcagcagg
     4861 ggatacagct gatacagaaa agtgtaatgg taattagtct atggatggga gcacatcttg
     4921 tcatatcggg agagataagc atcgggcagt taatcgcgtt taatatgctt gcaggtcagg
     4981 tcattgctcc tgttatcaga ctggctcatc tttggcagga ttttcagcag gttggtattt
     5041 ctgttgagcg ccttggtgat gtattaaata caccggtaga gaaaaagtca ggcagaaata
     5101 tactgccgga aattcagggg gatatcgaat ttaaaaatgt cagatttcgg tattcttctg
     5161 acggtaatgt tattttgaat aatattaatt tatacatatc aaagggggat gttatcggta
     5221 tagttggccg ttctggttca ggaaaaagta cattaacaaa actgcttcag cgcttttata
     5281 taccagagac cggacagatt ttaattgatg ggcatgattt atcacttgca gatccagaat
     5341 ggttacgacg ccagattggt gttgtattgc aggaaaatat actattaaat cgtagtatta
     5401 tcgataatat tacattagct tctccggctg tatctatgga acaggctatt gaggcagcca
     5461 gacttgcagg tgcccatgat tttattagag aactaaaaga agggtacaat actattgttg
     5521 gagaacaggg cgttggtctt tcaggagggc agcgccaacg gatcgcaata gcccgggctc
     5581 ttgttacaaa tcctcgaatt cttatttttg atgaagcaac cagtgctctt gattatgagt
     5641 cggaaaatat aataatgaaa aatatgtcaa gaatatgtaa gaacagaacc gtaattatta
     5701 ttgcacacag gttgtcaact gtaaaaaatg caaacagaat aattgttatg gataatggct
     5761 ttatttctga agatggcaca cataaagagc ttatctccaa aaaagacagt ttatatgcat
     5821 atttatatca gttgcaggca taacgtatgc gcttttacat gaaaggttta tgggatttgg
     5881 tttgccgtta taagacggtt ttttctgatg tctggaaaat tcgtcacaca cttgatgctc
     5941 ctgtgagaga aaaagatgag tatgcatttc ttcctgctca ccttgaactc attgaaacac
     6001 cagtatccag acgttctcat tttgttgtat ggagtatttt attatttgta attatatctc
     6061 ttctcttatc tgttcttggg aaagttgaag tggtttcagt agcaaatggt aagtttactc
     6121 atagtggaag aagcaaagaa ataaaaccga tagagaatgc gattgtagaa aaaataatgg
     6181 taaaagatgg ctcttttgta aaaaaaaatg atccattagt tgaattgacg gtgcctggtg
     6241 ttgaatctga tattttaaaa tcagaagcat ctttgttgta tgaaaaaaca gaacaatatc
     6301 gctacgcaat tctttctgaa tcaatacaac ggaatgagct tcctgaaata aggataacag
     6361 attttcctgg cggagaagat aatgcgggag gtgaacattt tcagagggtt agctcattaa
     6421 taaaagagca attcatgaca tggcagaaca gaaaaaatca aaagcagctc acattaaata
     6481 aaaaaatagt tgaacgggat gcagcgcttg ctcgtgttag cctgtatgag catcaggtat
     6541 cacaggaagg aagaaaactc aatgatttta agtatttgtt gaataaaaaa gctgtttctc
     6601 aacattcagt tatggagcag gaaaatagct atattcaggc aaaaaatgaa catgcagtct
     6661 ggcttgcaca ggtttctcaa cttgaaaaag aaatagaact tgtgcgggaa gaactggcac
     6721 tggagacgaa tatcttcaga agtgaaatta tcgagaagca cagaaaatca acagataaca
     6781 ttgtgttgct ggagcatgag cttgaaaaaa acaggcagag aaaagcatca tcttttatta
     6841 aagctcctgt gagtggtact gttcaggagt taaatataca tacagaaggt ggtgttgtca
     6901 caacagcaga aacgctgatg attattgtcc ctgataatga tattctcgaa gtaacagcat
     6961 ctgtacttaa caaggatatc ggttttatac aacctggaca ggaggtcgtt attaaagtag
     7021 atgcatatcc atatacacgt catggttacc ttacagggaa agtaaaaaat ataactgcag
     7081 attctgtttc tgttccggat acagggcttg tatttaacgt gattatatct gttgatcgga
     7141 atgatataca gggagaaaga aaaaaaatcc ctgttacagc tggaatgact gttatggcag
     7201 aaataaaaac cggggtgcgt agtgttatca gctatcttct tagtccatta aaggaaacta
     7261 ttaatgaa
//
LOCUS       DAZFTN010000187         7242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFTN010000187 DAZFTN010000000
VERSION     DAZFTN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(438..1574)
                     /locus_tag="ACHKV2_004748"
     CDS             complement(438..1574)
                     /locus_tag="ACHKV2_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6942479.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1577..1939)
                     /locus_tag="ACHKV2_004749"
     CDS             complement(1577..1939)
                     /locus_tag="ACHKV2_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6942480.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2476..4389
                     /gene="mtlA"
                     /locus_tag="ACHKV2_004750"
     CDS             2476..4389
                     /gene="mtlA"
                     /locus_tag="ACHKV2_004750"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO6942481.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4484..5632
                     /gene="mtlD"
                     /locus_tag="ACHKV2_004751"
     CDS             4484..5632
                     /gene="mtlD"
                     /locus_tag="ACHKV2_004751"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6942482.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5632..6219
                     /gene="mtlR"
                     /locus_tag="ACHKV2_004752"
     CDS             5632..6219
                     /gene="mtlR"
                     /locus_tag="ACHKV2_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO6942483.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6231..6440)
                     /gene="yibT"
                     /locus_tag="ACHKV2_004753"
     CDS             complement(6231..6440)
                     /gene="yibT"
                     /locus_tag="ACHKV2_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO6942484.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6725..7087
                     /locus_tag="ACHKV2_004754"
     CDS             6725..7087
                     /locus_tag="ACHKV2_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO6942485.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1861 a         1800 c         1818 g         1763 t
ORIGIN      
        1 tatattaata actcactctc caggagtttt ttaacttagt tttataatga atacgataat
       61 taaaagtgaa gaagaaaata gttgtataca tatattccat gaccctacat tgtgatctat
      121 agattaaata ttttttaaat gtgactgtca tccttcagat aataaagcca tcatcgcaat
      181 agatggaaaa gcactcctgc tcttttttga ttagagccgt cgcaggagga acaactcata
      241 tcatcattgc gatctcgacg atgtatagtc tcatcctgcg gcagaacaag acggataaaa
      301 aatccaatga gagcacactc atccagaact tattaacata atgaatatta aagaaaaatc
      361 atcgcagcta atgaagtaat atgcaaaaat aattagtcct tacaaggagc aaagttccat
      421 aatctccccc ctccccctca atgatccaaa taaagataat gcatccagct ggtcattctt
      481 agcagcactt tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg
      541 gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc aatggttccc
      601 agcacaccgt ccgtgccggg cacgaccgtt aatgattgca ataccccctg cgcctgataa
      661 gaaccgcctg gcacgacagg taaaatacta gtcagtttgc cgtgaaacac ctgcccaggt
      721 agcgcgttaa acaccacttc cgcatcatcg ccgggtttca gacgtaacag cgagttttgc
      781 cgaaattggg cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc
      841 ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct gagtaacgta gccattgctc
      901 ggcgcgcgaa tgacagtctg ctcaaggtta tattttgctt cagtaagttg cgctcttaag
      961 ctcacaatct gcgattgctc gccgttaacc atactgtcga gctggctctg gatctgcgcc
     1021 tgctccgcca ccgagccttt caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg
     1081 tcgatgtcac gttccgagaa cggattcacc gccgcctggc tgcctttcaa gtaacgttga
     1141 taatttttaa acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca
     1201 gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt
     1261 ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttttga
     1321 ataagctgat tattcttgtc agtgacttca gtaacaattc ccgtcacctg tggcgtgata
     1381 gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaat
     1441 aaaatcaaac cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgtccactga
     1501 tttaccggaa tgcggaagat tttaaagacc gcccacgcca gcgccacgta agttaaaaca
     1561 atcaatagat ccataattag atctccggga acgtggaata gtcagtcttt ttctcggcgg
     1621 cgagttgatg ctccagccgg gaaattcggt cagaaagtga ggcgatttcc gggtcagtcg
     1681 ctttctcctg cgacgcaaca tgtgactgca taccccagcc acgctccggt tgatagagcg
     1741 tcgcccagat ccacagaaac ggccagataa catgcagagt aaacaggctc acccagcccg
     1801 ccgtatgaat agcgtcggca tgggggtgat tgcgcttttt ggcaatcaga taagggatgt
     1861 catgtatcgc gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg
     1921 cgaaatagtt tagaaacata tctgcctcgg attcacgttt atcagtgttg tttttgggct
     1981 ggcagccaga agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc
     2041 ataacaccaa agaataattt ttagaggtga tgagttgctt agttacataa cgattgtatg
     2101 acgaaggcat aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga
     2161 ttcagatcac aaagattcaa caaaccatca aaacaaaaat gtgacactac tcacatttaa
     2221 atgccatttt tagcgaaaat cgccgccttg ttgctttttt acacaagcgt tttgtgatga
     2281 acgtcacgtc aattacctct ctacccccta tatttatgtg attgatatca cacaaaaggc
     2341 cgtcgactgg acagttaacc gattcagtgc cagatttcgc agtatctaca aggtccggct
     2401 acctctgccg ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata
     2461 agaaggggtg tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc
     2521 ctcagcaaca tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg
     2581 ttatttattc caacagggtg gttaccgaac gagacgctgg cgaagctggt cgggccgatg
     2641 atcacttatc tcctgccgct gctgatcggt tataccggtg gtaagctggt aggcggcgaa
     2701 cgtggcggcg tagtcggtgc catcaccacc atgggcgtta tcgtcggcgc agacatgccg
     2761 atgttcctcg gttctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac
     2821 cgctgggtag acggtaagat caaatccggt tttgagatgc tggtgaataa cttctccgca
     2881 ggcatcatcg ggatgatcct cgctattctg gcattcctcg gcattggccc gattgttgaa
     2941 accctgtcca aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga catgctgccg
     3001 ctggcgtcta tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac
     3061 ggtatcttct cgccgctggg tattcagcag tcccatgaac tgggtaaatc aatcttcttc
     3121 ctgattgaag ctaacccagg tccaggtatg ggcgtgctgc tggcgtacat gttctttggt
     3181 cgtggtagcg ctaaacagtc tgcgggcggt gcggcaatca tccacttcct gggtggtatc
     3241 cacgaaatct acttcccgta tgtgctgatg aatccgcgtc tgatcctcgc agtcatcctc
     3301 ggcggtatga ctggcgtgtt cacgctgact atcctgggcg gtggtctggt ttctccggca
     3361 tctccgggtt ctatccttgc tgtactggcg atgacaccaa aaggtgctta tttcgctaac
     3421 atcgcgggtg tgtgtgcggc gatggctgtc tccttcgttg tctctgctat tttgctgaaa
     3481 accagcaaag tgaaagaaga agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg
     3541 aaagctgagt ctaaaggtgc atctccgctg tctgcgggcg atgtgactaa cgacctgagc
     3601 cacgtacgta aaatcatcgt tgcctgtgac gccggtatgg gttccagtgc gatgggcgca
     3661 ggcgtgctgc gtaagaaaat tcaggatgca ggtctgtcac agatttcggt cactaacagc
     3721 gcgatcaaca acctgccgcc agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa
     3781 cgcgctatgc gccaggttcc tcaggcacag catatttcgc taaccaactt cctcgacagc
     3841 ggcctgtaca ccagcctgac tgaacgtctg gttgctgccc agcgacatac tgaaaacgaa
     3901 gtgaaagtga aagacagtct gaaagacagc tttgacgatt ccagtgctaa cctgttcaaa
     3961 ctgggcgcgg agaacatctt cctcggtcgc aaagcagcaa ccaaagaaga agcgattcgt
     4021 tttgctggcg agcagctggt gaaaggcggt tacgttgagc cggagtacgt tcaggcgatg
     4081 ctggatcgtg aaaaactgac cccgacttat ctgggtgagt ctatcgcggt gccacacggt
     4141 acggttgaag cgaaagatcg cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa
     4201 ggcgtgcgct tcggtgaaga agaagatgac attgctcgtc tggtgattgg tattgctgcc
     4261 cgtaacaacg agcacattca ggttatcacc agcctgacca acgcactgga tgatgagtcc
     4321 gtcatcgagc gtctggcaca caccaccagc gtggatgaag tgctggaact gctggcaggt
     4381 cgtaagtaat ccaatcctac cctctcctca cggagagggt ttgggtgagg gaaaagcctc
     4441 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4501 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4561 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4621 tcaggttcat gtggtcggtg aaaccgagca ggtagatacc gtttccggcg tcgatgctgt
     4681 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc
     4741 cgttggcccg gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg
     4801 taaagaacaa ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg
     4861 taccacgcag ctgaaaggtc atgtgatgaa cgccctacca gaagacgcca aagcgtgggt
     4921 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc
     4981 ggcaactaac gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa
     5041 aacgcagttc aaaggcgcac tgccgaacat cccaggcatg gagttaaccg acaacctgat
     5101 ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct
     5161 cggaaaactg gccggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc
     5221 ggtggtaaaa ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga
     5281 cgcagacaaa catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct
     5341 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5401 tctgatcaag ccattgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca
     5461 ggggattgct ggtgcaatgc acttccgcag tgaagacgat ccgcaggctc aggaactggc
     5521 agcactgatc gctgacaaag gtccgcaggc ggcgctggca cagatttccg atcttgatgc
     5581 caacagcgag gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac
     5641 caggcgcagg acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     5701 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     5761 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     5821 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     5881 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac
     5941 cgccaggaat acgaagatgc ggagctgctg atggcattgc gtgaagaact aaatcacgac
     6001 ggcaacgagt acgcctttac cgacgacgag atccttggac cctttgggga actgcactgc
     6061 gtggcggcgt taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg
     6121 caaattcagc gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg
     6181 atttccagaa tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc
     6241 tctacctgct tcggccgata aagccgacga taatactcca gacgttgtag atataacggc
     6301 acgctttcat cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac
     6361 tcccggaacg cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6421 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6481 cgccaatttt agtttaggtt cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc
     6541 aataatcatc cgctgctgtt tattatcttt tttctataga tttcttaatt aatcaataaa
     6601 ttgcgtagtt tttaagcatg ttaatgatta cgttacagaa ctattgcaat gtcatcagtt
     6661 gtgaacaaag cacctggtcg cgcatactag gggctataaa tttatcttta tcagaagcca
     6721 tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc agcgatcgtc tggacgccat
     6781 ccgccaccag caggccgatc tgtcgctggt tgaagccgca gacaaatatg ccgagctgga
     6841 aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg cgtgaagttc atagccaaaa
     6901 actgagtaaa gaagcacaga agctgatgaa gatgccgttt cagcgcgcaa ttaccaaaaa
     6961 agagcaggct gatatgggca agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc
     7021 aatgaccgcg ctgggccgtg aaatgggcct gcaggagatg actggattct ctaagactgc
     7081 gttttaagcg cctcagacga ctggttcgat catatcaacc cgcatctggc ttgccttagc
     7141 tggtcaatgc ggggactctc ctgtctactc gcctaattac caaacaattc actcccttta
     7201 cgtcgattta ccgctggttg atattctgaa aatgcatttt ta
//
LOCUS       DAZFTN010000188         7058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFTN010000188 DAZFTN010000000
VERSION     DAZFTN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1037)
                     /locus_tag="ACHKV2_004755"
     CDS             complement(<1..1037)
                     /locus_tag="ACHKV2_004755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO6942486.1"
                     /translation="MKTWGCGGLELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKP
                     HERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAV
                     DANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRIS
                     PETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSR
                     NIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLS
                     LPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPK
                     KTCLAQYTQHEL"
     gene            complement(979..1173)
                     /locus_tag="ACHKV2_004756"
     CDS             complement(979..1173)
                     /locus_tag="ACHKV2_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942487.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRFGTMEERNRLQ"
     gene            complement(1194..1475)
                     /locus_tag="ACHKV2_004757"
     CDS             complement(1194..1475)
                     /locus_tag="ACHKV2_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942488.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(2014..2100)
                     /locus_tag="ACHKV2_004758"
     tRNA            complement(2014..2100)
                     /locus_tag="ACHKV2_004758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2064..2066),aa:Leu,seq:taa)
     gene            complement(2113..2186)
                     /locus_tag="ACHKV2_004759"
     tRNA            complement(2113..2186)
                     /locus_tag="ACHKV2_004759"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Cys,seq:gca)
     gene            complement(2240..2315)
                     /locus_tag="ACHKV2_004760"
     tRNA            complement(2240..2315)
                     /locus_tag="ACHKV2_004760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2280..2282),aa:Gly,seq:gcc)
     gene            complement(2467..3015)
                     /gene="pgsA"
                     /locus_tag="ACHKV2_004761"
     CDS             complement(2467..3015)
                     /gene="pgsA"
                     /locus_tag="ACHKV2_004761"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO6942489.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(3072..4904)
                     /gene="uvrC"
                     /locus_tag="ACHKV2_004762"
     CDS             complement(3072..4904)
                     /gene="uvrC"
                     /locus_tag="ACHKV2_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO6942490.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(4901..5557)
                     /gene="uvrY"
                     /locus_tag="ACHKV2_004763"
     CDS             complement(4901..5557)
                     /gene="uvrY"
                     /locus_tag="ACHKV2_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO6942491.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            5699..5815
                     /locus_tag="ACHKV2_004764"
     CDS             5699..5815
                     /locus_tag="ACHKV2_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6942492.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            5853..6029
                     /gene="yecU"
                     /locus_tag="ACHKV2_004765"
     CDS             5853..6029
                     /gene="yecU"
                     /locus_tag="ACHKV2_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO6942493.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            6016..6240
                     /gene="yecF"
                     /locus_tag="ACHKV2_004766"
     CDS             6016..6240
                     /gene="yecF"
                     /locus_tag="ACHKV2_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO6942494.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(6308..7030)
                     /gene="sdiA"
                     /locus_tag="ACHKV2_004767"
     CDS             complement(6308..7030)
                     /gene="sdiA"
                     /locus_tag="ACHKV2_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO6942495.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         1845 a         1792 c         1577 g         1844 t
ORIGIN      
        1 agttcatgtt gagtatattg ggcaagacat gtctttttag gaaagtactg ccgaattagc
       61 ccatttgtgt tctcatttgt tccccgctgc caaggactct gaggatcgca gaagtaaact
      121 ttaacgccgg tgctgacagt aaattctaga tgtctggcca gttccattcc tctgtcccat
      181 gtcagtgatt ttctgagttc tgacggtaaa ctcaggaatt tgtcggtaag agcctgattt
      241 actgagacag aatctttgcc cctgagtctg aggatgatcg tataacgtga ttttcggtct
      301 acaagtgtgg ctatatgaga gttttttgta cctgagacta aatcgccctc ccaatgccct
      361 agagagcgtc tgttatcgat atttcgggaa cgttcgtgaa ttggtgttcc gttcactatg
      421 ttaatcgtac ctctttcgcc tttgcgggta tgacgcctgc catggcgaag gctatgcgac
      481 cgtcgcagat gctgtatatt caggtggtgt agcgcttcac ggctacgaaa gtacagcgtt
      541 ttataaattg tctcaggtga tattcgcagc gttttttgac gtggttttgt tcgccttaac
      601 catcctgata tttgctctgg agaccatttc atctccagct tttccagaac aagctttcgc
      661 aatggtaaat tttgatccag taagcacggt tttggccttt tcgccattct gttggctcgg
      721 ttattagcat caacagcttt gtaatagcgt ctgccccgat tacgctgaac ttcacgtgag
      781 atcgtcgaag gactgcgatt cagcgcagta gctatcgcac gaatgctcat tttggctgac
      841 aaaccagctc gtatctcctc gcgctcagac agtgtcaggt gagctacagc ccgcttacgc
      901 tcatggggtt ttatgccgcc agtatccctt aacatagtga agatcgttcc gggttttgaa
      961 cccaggatat tcgctatttc actgaagcct gttccgttct tccatagttc caaacctccg
     1021 caaccccatg ttttcacata actgttgcgt tgaccaattg aatctacact tacattgttg
     1081 atgaaatcgc tcacgaagga ccaccggaca ggctttcagc cagaagcgca gattttcggg
     1141 atgtgaaccg ttaacggcac ataatgctcg cacaacaaac ctgttaaaag agattactca
     1201 gcattctcct gcttccattg ccggatcatt tcatcggtaa catcaccctc ataacatacc
     1261 acgtcatacc ctccattacg gctatatgca ctgcgtccac cacatctact accattcctt
     1321 gcatgattat aaggacacgc acaattgcct ggataagatt caatggattc tttaattatt
     1381 tcttttttga tctgagcatc agactttcct gttgctgcat atccactaaa agatactaaa
     1441 caaaggcaga ttgccattaa taaaccacat cgcataccga tacactcaat tactaaccaa
     1501 tcatagccca catcataaaa tcgagatgag gagcacaata cctttaacaa gaaggtgtca
     1561 taacgataat atgtcgtgca attcatcaaa aaaaagatct atatcaaaga gatagaactc
     1621 ttatcagtgc ttcagacatc ggcattcgag gcagcagaaa aacagtaaat ctgacgaatt
     1681 tgccttttcc caagatgttg tcagattaaa aagatagatt agcgcaggta cctgttggtg
     1741 gattgcagga ggaaaaataa agtcgagtgg aaaaatccca gaaaaatttt tgtttaacag
     1801 aggtcctggt tcactatgcg aaatacggag gagctgcaga agaattctgc ttcatcctta
     1861 gagtttaaac agcacgtgca ctaccattgt ttctgcatat cgccacacca tcgccactct
     1921 accgccattg cacaaaatac aaatacgaaa aaaccacccg aaggtggttt cacgacactg
     1981 cttattgctt tgattttatt cttagctttc ccatggtacc cggagcggga cttgaacccg
     2041 cacagcgcga acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc
     2101 tcgggaagaa agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg
     2161 ctacataacc gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctg
     2221 acgccgatgt cttttaaact ggagcgggaa acgagactcg aactcgcgac cccgaccttg
     2281 gcaaggtcgt gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac
     2341 ttgattttat tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc
     2401 tggcgcgatg agtcaacgat atttttcacc acttttgatc gtttgctgaa aattgcgccg
     2461 aaacgatcac tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca
     2521 cagagtcagt accgcagcca caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt
     2581 cggacgccac agcagccatg ccaacgccac catctgggca gtggttttca ctttcccaat
     2641 ccaggagaca gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga
     2701 aataataatt tcacgggcga tcatcgttgc cgccggtaag gtcacccacc agctgtggta
     2761 atgctcggtt accagcacca tggcgatagc cacgagaact ttatctgcta cagggtcaag
     2821 gaaagcacca aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc
     2881 agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccaag tgacaggcag
     2941 ataaaagacc aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg
     3001 gatattaaat tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg
     3061 ctacagagac atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata
     3121 cccggcactt ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata
     3181 tatttcaaca acatttgccg gcgttttggc ccgacgcctt caatggtttc cagggaactg
     3241 gtatttttga ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc gtgtgattca
     3301 tcgcgaatat gctggataac atgcagcgcg ggagaatctg gcggcaaact gaatccctca
     3361 ccttccggct caaagaacag cgtttccagc ccagccttac gatctgctcc tttggcaaca
     3421 ccaagtagca gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc
     3481 gcctgcgcaa gctggccttt gccgccgtcg ataagtatca catccgggat cttactgtcg
     3541 tcgatggctt taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc
     3601 ggcgtgatgc cagtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca
     3661 aacaccacac aggaagcgac ggtttgttca cccatggtat ggctgatgtc aaagcactcc
     3721 atccgcttca cttccggcaa tttcaacacg ctggcaagtg ctgtaagccg ctggtgaacg
     3781 gtagattgct gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc
     3841 aggtaacgcg ccctatcgcc acgaggtttg gtttgaacat taatcttgcg tcccgccagt
     3901 tctgaaaggg aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca
     3961 cccggtaagg tgcgcatctg gctgccttgt aaatagaact ggcctacgaa ggtttctacc
     4021 acctcgctca gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact
     4081 ttgccctgac gaatgaacaa tacgtggaca caagccatac ccgcatcgaa cgccacacca
     4141 ataacgtcga ggtcgtcgcc ggtattggaa acgaattgtt tttcggtgac gcgtcgcacc
     4201 gcctgaattt ggtcgcgaat acgtgccgct tcttcaaact ccagattctg actggcagtt
     4261 tccatacggc taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc
     4321 acatactcga cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc
     4381 agacagcgcc ctatctgata ttgcagacac ggacgcgagc gattgcgata aacactattt
     4441 tcgcactggc gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag
     4501 ccattcggga acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc
     4561 agacgcgggt gggtatcgcc actcaggaag ataaaaggat atgatttatc atcgcgtagc
     4621 aaaacgttgt aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg
     4681 gtttctgtat gagtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt
     4741 ttgcgcgaag cgaggttgct acggaaatag ctggaaagcc gttttttcag gtctttcgct
     4801 ttgccgacat agataaccgt accaccagca tcgtacatgc gataaacgcc aggctggctg
     4861 gttacggttt ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct
     4921 ccgcattaca cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt
     4981 ttagtttact gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct
     5041 gttctgagat ctcattgacc ttctggccct tcgtgatcat cagcataatc tgcaattcac
     5101 gttcagacaa actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaatgcca
     5161 tttgttgagc gatgtcagaa gcaatgtaac gctgcccaga atagacagaa cggatcgcac
     5221 tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc cgcagcaccg gcctgcatga
     5281 ctttcgctgg taaagggttt tctgtatgga cggtaagcat gatgattttt acatcagctg
     5341 tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca
     5401 ttagcaccac gtcaacggca tttgcccggc accacttaac ggcgtcttca ccgcacgatg
     5461 cctcaccgac gacttttata ccctttatat cttccagaat gcgtcgtatc cctgcgcgca
     5521 ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga
     5581 taacgctact gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt
     5641 taaaaatggt gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat
     5701 ggtcacgccc gtcaccaacg gcttaccgga tcgtttcaca gtaagccctg cgacgaaatg
     5761 tgacaaaaat tacctttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta
     5821 attaacccga acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata
     5881 ttaacgcatt taatagcacc gttgacggca cgctcaaaaa acaaccactg ctatttttag
     5941 caaagcttat ggtatactcc gccgccttaa catttttcac cgcaaatttt cattgcaaca
     6001 tgacgaggaa aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg
     6061 gcaaatgaag tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa
     6121 gctgacgcgg gccgcgtgat gttaaagatg gaacaacagc ttgcgctgat cgaagacgaa
     6181 acccaggctg cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa
     6241 tataaaaccg gctgatagcg tgcctttcag ccggtttttg catctggcac gcaggacaga
     6301 aaagagatca aattaagcca gtagcggccg cgtaacaggc aacctgggtc ttatttggtg
     6361 cattaatttt tttctgcatg tttttctggt ggaaattgac cgtattctca gagattgaca
     6421 aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccacctcaga atttcttttt
     6481 cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag
     6541 ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg
     6601 gtatttcgcg cgcgctgcaa cgggaaaagg acaaaaagcc cagcgcccgg tttggcagca
     6661 ttaaatactg agtgacaccg cggcgtaaac catgtgcgcg cgcggcttcc cataacggct
     6721 gtgcttcgtt gaataagtca tcattccaca ttagatggcc ctgactaaag ttttcagggt
     6781 tcagcaccgg atcaattgcg agaaagtttt ttgcctgata ataactaacc cacgcctcag
     6841 ggtaattggt gtagaaagcc actttaggtc gagtgaatgg taccgggtgg cggacacata
     6901 acgaatagta atcgtactcc agctgctgag cctgaagctc aatttcatgg tagacctctt
     6961 ctgcggtctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg
     7021 tatcctgcat agtaaaccgc aacgcccctg agagtgag
//
LOCUS       DAZFTN010000189         7056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFTN010000189 DAZFTN010000000
VERSION     DAZFTN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHKV2_004768"
     CDS             complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHKV2_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO6942496.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHKV2_004769"
     CDS             complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHKV2_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO6942497.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHKV2_004770"
     CDS             complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHKV2_004770"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO6942498.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4504..4788)
                     /locus_tag="ACHKV2_004771"
     CDS             complement(4504..4788)
                     /locus_tag="ACHKV2_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO6942499.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5160..6500
                     /gene="fadL"
                     /locus_tag="ACHKV2_004772"
     CDS             5160..6500
                     /gene="fadL"
                     /locus_tag="ACHKV2_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO6942500.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6865..>7056
                     /locus_tag="ACHKV2_004773"
     CDS             6865..>7056
                     /locus_tag="ACHKV2_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942501.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRGNDDP"
BASE COUNT         1804 a         1878 c         1646 g         1728 t
ORIGIN      
        1 gtaaaaatac attacaactt agtctcattt cgtcagaaat taatagggaa gcagaggaaa
       61 aagtgcgatg aattctgtat cgaagcgtat caactccgtg aagggttttg agtgaatttc
      121 tactattgca aaaagacaaa tgcccgatgt acagcttaca tcgggcattt tttcaaacga
      181 atcagatagc tttcgccatc ttcaaattac atgggctcat ctgccagttg aactcgcctt
      241 ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt
      301 cgcccggaca taactcggcg accagatacc caactaacgg taagtgggag ataaccaaca
      361 ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc
      421 cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga caatccccaa
      481 cttcctccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca
      541 ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaaccg ttagtggtca
      601 gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa
      661 cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag
      721 agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt
      781 tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca atccgccatt
      841 cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa
      901 aaactttccc cacgcgcacc catctcgact aaacgctcgc aaggggtaaa acgtgaacca
      961 tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata
     1021 tagcgaaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc accaatatcc
     1081 ccgtcacgca cgctacggat aacctgctca tcaacacaac gtacagcttc attcagcatc
     1141 aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca
     1201 atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt ctgaccataa
     1261 agatagaaac ctcgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca
     1321 acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta
     1381 gtcccggtgt cgattcctac ctcatccaaa agttggattg gacctaccgg aaaaccaaat
     1441 ttcactagcg cggcatcaat gtgcgctacc cgttcacctt cggtcagcat gcggatagct
     1501 tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca
     1561 atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgtgctgat
     1621 gtacccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact gaagaaatgc
     1681 aggccgataa cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat cggtaaagat
     1741 gacgtattcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc cgccaccatc
     1801 tgttgtttca attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag
     1861 ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg
     1921 agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt
     1981 atgccctgcg ggttgatatc tttaattctg accggaatcc ccgctttaca agcagtgaca
     2041 taagcaatac cgccgcccat caagccacca cctaaaatcc ccacgctgtt taatggcgca
     2101 ggcggcgcat cactgccggg atctttcttc acttccgtac tggcaaaaaa gatactacgc
     2161 aaagcttgcg attgtggcgt catcgccagt tcgccaaacg cccgtgcttc agcgtcatag
     2221 ccactgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc
     2281 ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca
     2341 cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga
     2401 tccttctttg ccagctcaac agcggcttcc agcagaatgg agtgcggaac aacatcatcc
     2461 accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag gatcatctct
     2521 aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac
     2581 aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt acaaacgcga
     2641 ccgtggcacg ccagcgccaa ctccagccca ccacccaggc aagcgccatg aatagccgcg
     2701 ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccaga
     2761 gcttccgctt cttgcgccgt tttgcaattg ccgatcatgt tgatgtctgc gccagcaatg
     2821 aagttgtccg gtttagcgga gacaaacacc acgcctcgca actctttgtt ttcacggagt
     2881 tgcttaataa tggcgcgcac ctgcgaggca aactccgcct tcagggtatt cattttctca
     2941 cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct
     3001 gatgtcattt ccattattcc gcctccagaa ccattgccgc gccaagccca ccggcagcac
     3061 aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa
     3121 tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg ccaagcacgt
     3181 taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagcgct tcacgagcaa
     3241 aacgttcact acccagtagc tgaatattgg ccaacgtctg ggcggcaaag gcttcgtgca
     3301 tatcaatcaa tgtcagatct gccattgtca aaccggcacg ctccagcgcc agcggtgttg
     3361 accaggctgg accgagcaac atgtcctgcc agacatcgat agcggtaaat gcgtagctgc
     3421 gcagatatcc cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca
     3481 ccgccgctgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt ccgtgtttac
     3541 gatcaaacgc cggacgcagc tttgcgaaat cagcaagcga ggaattaccg cgaatattgt
     3601 tgtcttccgc gagcagttgt ttataaggag ggataaaggc agtcatcacc tcttctttga
     3661 gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt
     3721 gctgttctcg ggtgatgccg taggttttcg ccatttgctc tgcagtgtcg cccatccgca
     3781 agccggtaga atattctgct accgcaggtg gtacgggcat taagtcgcgc aaacgcaggc
     3841 gagagaagag tttcagacgc tggctcattg tgcgggcttt gttgacatca accagcacgc
     3901 gcgccagttt tttactgacg ccaattggca ataccgaaga ggaatctgcc ccaccggcaa
     3961 tccccgctcg gatagtcccc gccatcaggc tttctacgac gtttgcaacc gcctggaaac
     4021 tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt ccagtaccga
     4081 gaacaatttc acgcgcaatg ttgggagctt caggcatttg tacgacctga ccaaagacca
     4141 gttgttcaat cacttcggcg gggatctcgc tgcgtgccag cagttcgcct accaccatct
     4201 tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac
     4261 gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac
     4321 ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat agtcttaacc
     4381 atttttttac atttagccaa gcggagaaaa gggaaattgg gagctatgac acagtgaaga
     4441 caggagaaag aaaagaaaac gcccctacct gttgctggca ggagcgtact tatccgagag
     4501 gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc
     4561 gatatccagg cgcacaccat cagactcttc agtgaaagtc ggggagattt tgcacggttc
     4621 ggattccacg ctacgggctt tctcagtcag cgccgccagc gtttgttctg cttctgcgcg
     4681 gtttgcaaac acgcggctgt aagacgctgt gcagtcggag ttgtccataa tggtgccaac
     4741 atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct
     4801 ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg
     4861 ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga
     4921 tctaaaacaa aattcacccg aatccatgag tgcgccacct ccaaattttg ccagctggat
     4981 cgcgtttctt agatcatatt tgaaaaaaga tagaaacata cttgcaacat tccagctggt
     5041 ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg
     5101 cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca
     5161 tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca
     5221 cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg
     5281 cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgtcagccgt aaccccgcat
     5341 tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gacccggatg
     5401 taaatatcag cggaacgtct ccatctggtc gtagcctgaa agccgataac atcgcgccta
     5461 cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt ggttggggcg
     5521 cttctattac ctctaactat ggcctggcaa cagagtttaa cgatacttat gcaggcggct
     5581 ctgtcggggg tacaaccgac cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct
     5641 taaataatgc atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg
     5701 aacgtttcgc aggcgatctg gggcagctgg ttgctggtca gattatgcaa tctcctgccg
     5761 ggaagactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac agtaatacca
     5821 aaatcgctca tctgaacggc aaccagtggg gctttggatg gaacgccggt atcctgtatg
     5881 aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa attgacttca
     5941 aaggtaacta cagcagcgat cttaatcgtg tgtttaataa ctacggtttg ccaattccta
     6001 ccgccacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct gaaatgtggg
     6061 aagtgtcggg ttataaccgt gttgatccgc agtgggcgat tcactatagc ctggcttaca
     6121 ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac acgctgttcc
     6181 agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact tattactacg
     6241 atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt ccggcacaga
     6301 atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg
     6361 cgtttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt cagagcgtga
     6421 aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta
     6481 actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc tatgcaggtg
     6541 actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc tgagttttgc
     6601 aaacagcctg atggcagcta gccctttcaa atcagtaagt ggtatccaca gacaaacctg
     6661 aagggaaacg catattttca ggcgttctgc tcgtccttct caaagagttt acttttctga
     6721 atttccagga tactcaaccc cctggctatt gtgcgctcat acacgcaaat taaagatagg
     6781 ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat atttgtaaca
     6841 aatcaatcaa catggaataa aatcatgcta tcatctattt caatcaacaa taccagcgca
     6901 gcttacccag aatccatcaa tgaaaataac aatgatgaag ttaatggatt agtacaagag
     6961 ttcaaaagcc tttttaatgg taaggaagga ataagcacct gtattaaaca tctacttgag
     7021 cttataaaaa acgccatacg aggaaacgac gatcct
//
LOCUS       DAZFTN010000190         7053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFTN010000190 DAZFTN010000000
VERSION     DAZFTN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(74..2857)
                     /locus_tag="ACHKV2_004774"
     CDS             complement(74..2857)
                     /locus_tag="ACHKV2_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6942502.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2914..5286)
                     /locus_tag="ACHKV2_004775"
     CDS             complement(2914..5286)
                     /locus_tag="ACHKV2_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO6942503.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5324..7009)
                     /locus_tag="ACHKV2_004776"
     CDS             complement(5324..7009)
                     /locus_tag="ACHKV2_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO6942504.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1825 a         1612 c         1587 g         2029 t
ORIGIN      
        1 tttataaaat tattacataa aaatagcgaa tattgctaaa atccccgcca acgatgtgtt
       61 gacggggctg ttattatttt ggcaataata ctccggtata agtatttacc ggatgagaaa
      121 gatattgttt aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct
      181 gctcagtcca tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta
      241 attgttgaac gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcgt
      301 tcagttcttg ctcactgatc cctttagtca aacgcttaac catcacttca ttcgctaacg
      361 ttaacagttc atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt
      421 tagcctgagg atcaaccgag aggcgagaag aaacgctgta tgccccagat gcctgttcac
      481 gaatattaac gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg
      541 tcgccagatt aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag
      601 gttcattttg ttcctttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac
      661 ctgcggctaa tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca
      721 cgagtttgtc ttctgcgaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa
      781 acaactggcg atcggcagcc agcgcatctg cggcagtaaa ctgtgcaatc tgattttctt
      841 gcagtaattt cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct
      901 ccgctggacg ctggtcgagc gttttcaatg cctgaatttg agcattttgt agcgatgccc
      961 aaatattgtc gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt
     1021 cagggttatt agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc
     1081 tcatggttac cgagttttcc gcactccagc gtttcaggct ggaagaggag agttcgccaa
     1141 cgccgcttcc gctaactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga
     1201 aacttaaatc gcctttattc gatacggcaa taatttgtag cttttgctct tcacccgcgg
     1261 attttgccag aataaccctt gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca
     1321 gcgtttcttt gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc
     1381 tgcctgggaa gatgtaagcc gccagctttt tgttggcgta ctccttttcc agcgccagga
     1441 tagccgcagg agacaatgct tttttggcgg caacctcatt gtttaccatt tgctcccaaa
     1501 atgcgtcctg gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt
     1561 gctgccacag acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat
     1621 ttaatgagct ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat
     1681 caaccgcatt tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag
     1741 aaaaaccatg ctgatcaatg gttgccaact cttccattaa tgcattcgca gcatcctgca
     1801 tattatcgtc tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa
     1861 cgctgcgcgc agtgccgcca gaaatagtct tcaactcgcc cgactgtata cgttcctgca
     1921 gacgttgatt gaacagctga actaacatgc tccattcagc ttgttcgata aagctttgct
     1981 catcgttcac ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc acccggtttt
     2041 ctttatcatt aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat
     2101 tttcagctgc tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt
     2161 ctttactgtc gatatcgcca accacgataa aggtcatatt atttggttga taccagcgtt
     2221 gataaaattg gagcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag
     2281 gttcacggtc taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc
     2341 gccatttcgc atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta
     2401 cttcgagttt ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt
     2461 gcagattttg tttctgcgta gttggcaaac tcacctgata caccgtttcg tcatagctgg
     2521 tataggcatt aacatcgcga ccaaaacgca ggcccattga ctcaaatgtt tcgatgactt
     2581 tattacccgg ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc
     2641 cgcgctcatt gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct
     2701 gatcctttgg atgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg
     2761 taattaactt ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag
     2821 ttgccactaa cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg
     2881 gcgaatttac ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca
     2941 gaaagtacgg ccactggcgt aggtcgccac accggtattc gttgtatcaa ccgcggtttt
     3001 gctatccagt acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg
     3061 tggcgtccag gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata
     3121 ctgcttgtaa tctgaatatt cgctgatgta ttgcgcattt gtcttaccca ggataatgcg
     3181 agctttacgc gcttcttgcc aggccagcgt attcgcccac accaacccgc tcggttgatg
     3241 cgtgaaatcc atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacgct
     3301 atcgtaagag acaagattac cgttataaac cacctggtta tcaccggtat tgctctcctc
     3361 ataaccattg tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgcgg
     3421 gttaatatct acctggctga tatgcagggg ttcggccagt tcaaaactga ggttgaacga
     3481 atgcgttttg gttttgccgt cgttgttata ttcagtaatg gtggttttag tcgcgctgtc
     3541 ggtacgactg cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcgcgcgc
     3601 aataacgttc ttaccgattt tttgctgcaa tcccattgcc agttcatcgt tataaggcgt
     3661 tttcaaatcc tgataacgcg tcagggtttt attacctgat accgattccg tccagctatt
     3721 gcggatatca cgtaatccca tatcaagaat attcccgcca tagtaacggt tataacctgc
     3781 tgtaatcatt gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat
     3841 attgtggttt gacagatagt tgtcatagtc ataccgcacg ccgggcatta atgacacatt
     3901 acgccagcta atgcgatccg ccatatacag tgtgtagttg tcaattccca ggctgccttt
     3961 acctttatgg taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc
     4021 agactggtta tggcgttcag tccacgcatc ggaatagatg tattccgcgc cgaagtaggg
     4081 ttgatgcgaa acattaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta
     4141 attatctaca gcctgggaaa tgtgtcctaa tccgccacgg gtgcaacgcc ctgtaatatc
     4201 accatatgta catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact
     4261 aatatgatcc caaccaacgg tggtacgtag tttggcccat gcgagctgcg tatccatatc
     4321 ccatgccata ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt
     4381 attataatca cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct
     4441 ggcaaaccag gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc
     4501 ggcgacaatg ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga
     4561 taaaccggcg gtaacgccaa agttatcagc gagttcctga ttaaacgaca aggtataaaa
     4621 gttcttttta aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct
     4681 cttgttattc tcattgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccag
     4741 tttcacgctg ctatcatcag cgttgaagcg tttgaacttt gcatcaatta ccccgccatt
     4801 gaagcgaccg aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct
     4861 gacatcaaga taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga
     4921 ctcattcgct gggttcaggt tattagtggc actaataccg tcaatcaaat aggcattctg
     4981 atagggcgac gcaccgtgaa tagagatttt ctccgggcga atatctccct ggttcaacga
     5041 ggtactttgc gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt
     5101 gccattcccg gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac
     5161 gggtacaggg acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt
     5221 gtcatcgacg gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg
     5281 cttcatttat ttatatctcc gtacagcagg gcttttattg tttttataaa accgcgctaa
     5341 tgtcacaaat atcatcggcc aggttccaga caccgggttg atgtgtaacc ataatgacgc
     5401 cgcttgtggg tagtttttca cgcactagac gcagtaagcg gatagcctct tgttcctcaa
     5461 gatgagaggt agtttcgtca agaaatatcc atttcggacg tcgtaaaatt aatcgtgcca
     5521 gggcgatacg ttgtttttcg ccgctggaaa gaatatctcc ccagcgatcg tggtcatgaa
     5581 tacgcgcagc caatttccca agaccaacct gatgcagtac ttcgctcaat gatttatcgt
     5641 ctacgggcag gggaagtgct ttacaaataa tctctttcag taagccggtt ttgattaacg
     5701 gtgtttgtga cacataccaa ctgtcggcag gagaagaaat atcaccttta aaccacggcc
     5761 agcagtggga taatgtttta agcagtgtgg tttttcccgc accagagtaa cctttcagta
     5821 atagccattt gcctggcgaa acatgaaagt tcaggttctc taatatgatc ttattatcag
     5881 gagtacgaat actcgcgtca gccacttgta ccgcatgttg gcaattttta ggcttattcg
     5941 tagggcgctg ttcggtgagt tgatggaact catacaagcg atcgataacc gcagccagtt
     6001 cagcaagttc atcatattta taaataaacc agcttagatt gttcgatacc agcataaatg
     6061 cctggcgcga tttcatcagt ccgcccagat tgatctgccc gctaataaac tgcggcaata
     6121 acagaaagta gggaagaacg ctaagcgagc gcgaataaat attctgccag taatcaagcc
     6181 accgctgacg attcattaaa cgatgccagt tctctttaat ggtatgaaaa ttgtcgctca
     6241 actcctggcg ttgtagagac tcagcattgc ttagcgcaat cagttctgcc tgcttattat
     6301 gctgcacaag attagttcga aatgtcgctt cgctacgttg tttttccaca ttaagcggac
     6361 gaatacgttt acccacctta tgggtaaata aagttccacc gatcacaatg agcacaacgg
     6421 tatagaccat atacccctgg atattccatt ctgttccacc aacagtgaat gagagcatac
     6481 ccgcgctttg ccaaagaata acggtaaagg tgatcagcat gctaagtgac tggatgaagc
     6541 caaatgaaag actgagcgtt ttgctgatca gtaagagaat gtcttcagcg atacgttggt
     6601 cagggttatc tgtattttta tgctcgccgt agatctgcgt gaagtagtag tttttatctg
     6661 caaaccagcg attaaggtag taatctgtta accattcgcg ccagcgaata gtcagtagtt
     6721 taattaacca tgttttgttt acggatatta atacaaagat ccccagcagg gcagggaacc
     6781 aaaggacaag ctgccaaagc ttatcggttt ctttctggct tagcgcattg aaaaaatcat
     6841 tattccagtc gtttaaccag acctgaattt taaccacgcc aaggatcatg gcgaggataa
     6901 taatgattaa caacactgaa gttttattat tcttacgcag ccaaaagggc ttcagcagac
     6961 agagatactt tgctatcaac atacaaaacg taatgggaat ggttatcatt agcgaaaatt
     7021 gattgtgcca tccaatgatt atctatggca atg
//
LOCUS       DAZFTN010000191         7045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFTN010000191 DAZFTN010000000
VERSION     DAZFTN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..673)
                     /locus_tag="ACHKV2_004777"
     CDS             complement(<1..673)
                     /locus_tag="ACHKV2_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942505.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSCALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQ"
     gene            complement(684..950)
                     /locus_tag="ACHKV2_004778"
     CDS             complement(684..950)
                     /locus_tag="ACHKV2_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942506.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            1107..1718
                     /locus_tag="ACHKV2_004779"
     CDS             1107..1718
                     /locus_tag="ACHKV2_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942507.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            2076..2255
                     /locus_tag="ACHKV2_004780"
                     /pseudo
     CDS             2076..2255
                     /locus_tag="ACHKV2_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2227..5292
                     /locus_tag="ACHKV2_004781"
     CDS             2227..5292
                     /locus_tag="ACHKV2_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6942508.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5292..5621
                     /locus_tag="ACHKV2_004782"
     CDS             5292..5621
                     /locus_tag="ACHKV2_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942509.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            5631..6329
                     /locus_tag="ACHKV2_004783"
     CDS             5631..6329
                     /locus_tag="ACHKV2_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6942510.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            6335..>7045
                     /locus_tag="ACHKV2_004784"
     CDS             6335..>7045
                     /locus_tag="ACHKV2_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6942511.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIC"
BASE COUNT         1779 a         1622 c         2023 g         1621 t
ORIGIN      
        1 actgcgccag aaattcgggc agccagttaa tatcttcggc ttttatttcc tggcgcttac
       61 ccggtgccag taaaagcagg cgttcagcgg cgttctgcgc cttgttaaag gccgcaatcc
      121 agcgtttcag cgttatttca ctcaacgcac agctgtttcc ctttttggcg ttcgccgtct
      181 caaccattgc cacaatgcgc tcgtccagtt gcgaacgtgc caggttgtca acgataaacc
      241 ggatagcttt cgcacagctg aaaccgggtt gttgcgcgac tttcatcact tcgctgacga
      301 tcgcgattcg tgcatcagcc acctggcgct ggttttcagt cagggcatta aggcgttcaa
      361 ccatcagttg tggtgatccg cgatatgcct ccaccgcatc aaccacggca ggcgagcgtc
      421 tggcctttgt caccaccgga gccggtgatt catcagcttt ttgcgtcatc agttgaaggg
      481 cataacgttc acgcaatact tgttgcgcgc cagaaggcaa acaatcgata ttaaattcga
      541 ttgcttttgt ccccttacgt ttgcgtaaca tggctgcatt tcccgcagct gcttttgtga
      601 gtcgatggcg aatgttatgt tccatcattg gtaaatcagg aagaccaaca cattcttttg
      661 ctgttaccca catacatccc ccatcaagcc acttctctgg cgtccacctg gtatctgctg
      721 ggccagatta cagcaggttc aacccctatt ttttcggcga tggcggcttc gtatttacgg
      781 cactggcggt aaaacacatt gcgcattgat ccgcttttta atcctaactc cctttccagt
      841 tcagggaggt ctgtgccttt gttttcaagg gctgcatata ccgctttagc agaccagtca
      901 cacccctctc gaacaaacaa attattcgat gcttgtctgg ataccctcat tgtgtgatcc
      961 tctcatgttt acctgaaccg ataatcaaaa ccggttatcg atttaggtaa atcataatcg
     1021 caaaataatt cgatatcaac agttttttgc gagaatatcg aaattaaatg cgatgtcgat
     1081 aatttctttg ttttataggg ggttatatgg ataaggcaga aaaaaccgta tccagtgcgg
     1141 aacggtttat tgatatggaa ggaataaacc gattttcaga acgcttagct atggctatgg
     1201 gaaacatgac aaacgtcgaa ctggcgaccc tttgtggact atctgaagcc acagtaagaa
     1261 attacaggaa ggggaaaaac ttccctcctt tggataaact ggccttaata gctaaagcct
     1321 gtaattgttc actcgaatgg ttggccacag gcaagaacga agatgaatgc ggaaaaatcg
     1381 aaaacaattg cgattcaggt tcgggtattg atcaggcgct tagctctcta agcccccaag
     1441 agaagcaatt aatccttaca ttcatcaggc gagaaggtac taacaacctt gtcaggctgg
     1501 cagcaatgaa tacatctaca atcagccagg atgcggtaga aagcatcatt gatgccttgc
     1561 cattacgtcc cgtactaaaa aatgcaataa agataggaat ggcgggaagt gaagaaacag
     1621 acaaagagat tttgcgcgtt attgagaagt acaactcgag caataacgcc acccaggaat
     1681 caggggcagt gacagtaaag aaaaatgttg ttggttaagc gccatcgcta tcatgaggtg
     1741 gtgctgtaaa tacatataga gatcatatga ctcatcccaa gataatcacg ctattgaata
     1801 gtaattcatg atgattatca tactgattat agttagatcg attatgtgag cattttggca
     1861 gtttgaagat gttttgaaga agattttaag ggtaaattag cagtatcaaa tgtgtcgccg
     1921 ttttttgatc ttttgactat ttgttgagca gtcggtatca aaaagagttt tccgtccgaa
     1981 gtatacatca cctgttttat ttcagcatct tacaggtgat ttcacctcct ttcactaaaa
     2041 cccttgtcgg tatcaaatga ttcacctggt tacactcgct gaaggcattg atcgtgagaa
     2101 tggtgtccgg tagcagtgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg
     2161 ggataccgga gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg
     2221 tgcgttatgg accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt
     2281 aaaaatgaaa ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg
     2341 tcttctgccc gtatgcagcg ttttatggag cgtcagactc aggccgcccg gcagacaatg
     2401 caggcggctt cttcggctgc aacagccgca tccgtccatg cgcagacggt ggagaagagc
     2461 gcacaggctc atgaacgcat ggcccgcgag gtggagcaaa cccgccagcg tatggaggca
     2521 ctgagccaga aaatgcgcga ggaacaggcg caggccatgg ctctggcgga ggctcaggat
     2581 aaagcggctg ctgcgtttta tcgtcagatt gacagtgtga aacaggccgg tgcggggctg
     2641 caggaattac agcgtattca gcaacagatc cgacaggcca gaaacagtgg cgggattggt
     2701 cagcaggatt atctggcgct gatttctgag gttacggcga aaacccgtgt tcttacacag
     2761 gctgaggaag aggctacccg acagaaagtg gcgtttatcc gtcagcttaa agagcaggca
     2821 acccgccaga atctttcttc ttctgagttg cttcgtgcta aggctgccca gctgggggta
     2881 agcagtgctg cagaagtgta tatccgcaaa atggagcagg caggaaaagc cacgcattcg
     2941 ctgggtctga aaagtgcagc agcccgtcag gagataggcg ttctgatagg tgaactggcc
     3001 cgcggcaatt taggtgcgct gaggggatcc ggaataacgc tggctaaccg tgccgggtgg
     3061 atagacacac tgatgtcacc gaaaggcatg atgccaggag cggttattgg cggtattgcc
     3121 gcggctgtct atggtctggg taaagcctgg tatgacggtc agaaggaggg ggaagaattt
     3181 aaccgccagc tgtcgctgac ggggcattat gccggagtca ctgccgggca gctgtggacg
     3241 ctcagtcgtg ctatttccgg gaatggtatc acgcaacatg ctgcagccgg tgcgctggct
     3301 caggtggtgg ggagtggtgc atttcgtgga aacgatatcg gtatggtggc gagagctgcc
     3361 gcacagatgg agcgatcggt tggccagtcg gtcagcgata ccataaatca gtttaagcgg
     3421 ctgaaggatg atcctgtaaa tgccgcgaag gctctggaca atgagctgca ttttcttact
     3481 gccactcagc ttgagcagat acgcgtcctt ggggagcagg ggcggtccag tgatgctgca
     3541 cggatagcca tgtctgcact ggcagaggaa accggtcggc gtactgcgga tattgataat
     3601 aacctcaatg cgcttggcag tacgctgaag tatctgtctg atttgtggag tcgtttctgg
     3661 gatgcggcca tgaatattgg tcgtgaagac tcgctggatg aacagattgc cgctttacag
     3721 gagaaagtgt cgcgggcgaa aagactcccc tggacggcat catcttctca ggttgaatac
     3781 gatcagcagc gtcttaacga tcttcaggag aaaaaacgcc agaaggattt gcaggatgca
     3841 aaagagcagg cagagcggaa ttatcaggag caacagaaac gccgtaatgc tgaaaatgct
     3901 gcactgaacc ggatgaatga aacggaagca gcacgacatc agcgtgaaat agcgcgtatt
     3961 aatgccatgc agtacgccga tcaggctgtc agggatgcgg cgatacaacg tgaaaatgaa
     4021 cgttacgaga aagccctggc atccggtaag aaaaaaacac gcgaaacccg taatgatgag
     4081 gccacccggt tattgctgca gtacagtcag caacaggcac aggtggaagg acagattgct
     4141 gctgccagac agtcagcagg cattgccacg gaaaggatga cagaagcgcg taaacagctt
     4201 ctggctctgc agcagcgcat cagcgacctg gacgggaaaa aactgacggc agatgaaaag
     4261 agtgtgctgg cccgtaaaga tgaactgatt caggcactga cgctgctgga tgtaaaacag
     4321 caggagcttc agaaacagac ggcactcaac gagctgaaga aaaaaacaat tcagctgacc
     4381 agtcaactgg ctgaagaaga gcgcgctcag cgtcagcaac atgacctgga tatcgccacg
     4441 gtgggtatgg gtgatcagca gcgacagcga tatcaggtac aactgagtct tcgccagaaa
     4501 taccagcaac agctggagca gttgaggcgg gatagtgagc agaaagggac atataacacg
     4561 gatgactaca gaaaggccga gcaggcgctg acggagagcc tgaaccgaca actgaatgag
     4621 aatcgccgtt actggcaaca gcttgaagtt gtacagggta actggaaaaa cggagtcctg
     4681 cgtgcatttc aggattttac cgtggatgca gataatacgg cagaaacagc agaacaggtg
     4741 ttctcgtcag ccttcagcaa catgggaaat ggcctggcaa cttttgtcac taccggcaaa
     4801 ctcaatttca aatccttcac ctcttctgtg ctgtcagata tggcgaaaat cctggcgcag
     4861 gcaaccatga tgaaatcgat aaaagggatt ggcagtgtac tgggatttga tctcagcagc
     4921 ctttccctga atgccaatgg ggggatttat cagtctgctg atttgagtcg ttacagtggc
     4981 acggtggtta accgtccgac gttttttgct tttgcaaaag gcgcgggtgt gatgggggaa
     5041 gcgggacctg aagccattct gccattgcgt cgtggtgctg acggtaagct gggggttgtg
     5101 gcggatattg ggggttcagg tatggcgatg ttttccccgc agtacaacat cgagatcaat
     5161 aacgatggca cgaacgggca gataggtccg gctgccctga aggcggttta tgacctcggg
     5221 aaaaaagcgg cagcggactt tatgcaacag caggcccgtg atggtggtcg gttaagtgga
     5281 gcatatcggt aatggagacg tttcactgga aagtgcgccc ggatatgaat gtggtatcag
     5341 agccgaaagt ggtgacagtg aagctgggcg atggttatga acagcgtcgt gcggcgggac
     5401 tgaataacca gttgtcgact tacagcgtga cgatacgtgt tcgtaaatgt gaacacccat
     5461 ctttaaaagc ctttctggaa cggcacggtg gcgtccgtgc atttcagtgg acgccacctt
     5521 atgactggaa accgatcagg gtggtttgtc gtaaatggtc ggcaagcgtg ggggcgctgt
     5581 gggtaaccat aacggcagat tttgaacagg tcgtggcata ggaggctctg atgcaggata
     5641 ttccacagga aacacatcat gagacgacac gcctcactca gtcagcccag gcggtgctct
     5701 gggaaatcga tctgacagag gtcggtggtg aacgttattt tttctgtaat gagcagaacg
     5761 aaaaaggtga gccggttacc tggcaggggc ggcagtatca ggcatacccc attcagggga
     5821 cgggatttga actgaacggc aagggcagtt ctgcccgtcc gacactgacg gtttctaacc
     5881 tgcacggcat ggtcacgggg atggcggaag acctgcagag tctggtcggc ggaacggtgg
     5941 tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac ggaaacagcg
     6001 acgccgatcc ggagcaggag gtgatcagcc gctggcgcat tgagcagtgc agcgaactga
     6061 gcgcggtgag tgcctctttt gtactgtcca cgccgacgga aacggacggc gctgtttttc
     6121 cgggacgtat catgctggcc aacacctgca cctggaccta tcgcggcgat gagtgcggtt
     6181 atcacggtcc ggcggtcgcg gatgaatatg accagccaac gtccgatatc acgaaggata
     6241 aatgcagcaa atgcctgagc ggttgtaagt tccgcaataa cgtcggcaac tttggcggct
     6301 tcctttccat taacaaactt tcgcagtaaa tcccatgaca cagacagaat cagcgattct
     6361 ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgcggc ttcgtggtaa gcacgccgga
     6421 gggggaaaga tatttcccct gcgtgaatat ctccggtgag ccggaggcgt atttccgtat
     6481 gtcgccggaa gactggctgc aggcagaaat gcagggtgag attgtggcgc tggtccacag
     6541 ccaccccggt ggtctgccct ggctgagtga ggccgaccgg cggctgcagg tgcagagtga
     6601 tttgccgtgg tggctggtct gccgggggac gattcataag ttccgctgtg tgccgcatct
     6661 caccgggcgg cgctttgagc acggggtgac ggactgttac acgctgttcc gggatgctta
     6721 tcatctggcg gggattgaga tgccggattt tcatcgcgag gatgactggt ggcgtcacgg
     6781 tcagaatctc tatctggata atctggaggc cacagggctg tatcaggtgc cgttgtcatc
     6841 agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca tcggtgccga atcatgccgc
     6901 catttactgt ggtgatggcg agctgctgca ccatattcct gaacaactga gcaaacgaga
     6961 gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc gggcatggcg
     7021 cgcatctgcc tttacgggga tttgc
//
LOCUS       DAZFTN010000192         6990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFTN010000192 DAZFTN010000000
VERSION     DAZFTN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..554
                     /locus_tag="ACHKV2_004785"
     CDS             <1..554
                     /locus_tag="ACHKV2_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942512.1"
                     /translation="TFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQTNS
                     VVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDASTG
                     VMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGARYF
                     VIDSAAATAGKITAVAEYTLSYL"
     gene            640..1341
                     /locus_tag="ACHKV2_004786"
     CDS             640..1341
                     /locus_tag="ACHKV2_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6942513.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1394..3916
                     /locus_tag="ACHKV2_004787"
     CDS             1394..3916
                     /locus_tag="ACHKV2_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6942514.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAP
                     VAGAAVKVIFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            3932..5266
                     /locus_tag="ACHKV2_004788"
     CDS             3932..5266
                     /locus_tag="ACHKV2_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942515.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5263..5952
                     /locus_tag="ACHKV2_004789"
     CDS             5263..5952
                     /locus_tag="ACHKV2_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6942516.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6098..6880)
                     /locus_tag="ACHKV2_004790"
     CDS             complement(6098..6880)
                     /locus_tag="ACHKV2_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6942517.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAG
                     VCNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1885 a         1722 c         1639 g         1744 t
ORIGIN      
        1 ctactttcaa aaaagcagct ctgctggcag ctattgcatt acctttttca actatggctg
       61 cgcctacagt cacttttcag ggtgaagtaa ccgatcagac ctgttccgta aatatcaacg
      121 gtcaaaccaa ttcagtagta ttgatgccga ccgtagccat ggctgacttc ggtgcaactt
      181 tagctgatgg tcagagcgca ggccagacgc cgtttacggt ttctgtgtct aactgccagg
      241 ctccaactgg tgcagatcag gcaatcaaca ccaccttcct gggctacgac gttgacgcta
      301 gcacgggtgt tatgggaaac cgtgatacca gcagcgatgc ggcgaaaggc tttggcattc
      361 agttaatgga ttccagcact tctggtaacc cagtaactct ggctggcgcg actaacgtac
      421 cgggtctgac cctgaaagtt ggcgataccg aagccagcta cgacttcggt gcgcgttact
      481 tcgttatcga tagcgctgct gccactgccg gtaaaattac cgctgtcgca gaatacaccc
      541 tgagctacct ctaattaatc tactggctgg cagtaaatgc cagccagtat ttcttcgcac
      601 aattaatagt gatttttcat ggacatggaa aagataagaa tgaacatttt tgcatattta
      661 ctggtacttg tattttccat gagcatgagc agcagcgcgt ttgccagcgt ggtaatgacc
      721 ggaacccgta ttattttccc tggtgacgca aaggaaaaaa ccatccagtt gcgaaatacc
      781 agcgatcagc cctatatcat taatatccat gttgaggatg aacgtggttc tgacaagaat
      841 gtaccgttta tgccaacccc gcagacattt cgcatggaag ctgccgcagg tcaggcgtta
      901 cgcctgctct acactggtaa taatttaccg caggatcgcg agtctgtttt ctggtttagt
      961 ttcagtcaac taccttatct gaataagaat gataaaagtc agaaccagct catcctggcc
     1021 ctgactaatc gagtcaaaat tttctatcgt cccagctcga ttgtcggtaa atccagtgac
     1081 gcacccaaaa acctgactta ccaggtaaaa cagaaccgca ttgaagtgac gaatcccacg
     1141 ggctattacg tcacaattcg cgccgctgaa ctgcttaata atggtaaaaa agtccccctc
     1201 gcgaattcgg taatgattgc tcctcaaagc acaactgaat ggacactacc ctctggcatc
     1261 agtgtcgctc ccggtgcgca gatccattta gtgaccgtca acgactatgg cgtaaatgtt
     1321 acgtctgagc atgccttata acctctttct ccttttttga gcggtatcct ttttcacgca
     1381 cgagtacgtc gagatgaata aatccgttgt gtcaatttct gcggcaatgt tggttttact
     1441 ttgccaaccg gtcatgggga gcgaaatctc acccgcaaca ccgtcagatg aagacaacta
     1501 cacctttgac ccgcaactct tccgcggcag cagatttagt cagtcgtcat tagcaaaact
     1561 gacaacacgt gagtctgttg caccgggcaa ttataaaatg gatatctaca ccaacaataa
     1621 gttgtcaggc agttggaatg tcacgtttaa agaagccgct gatggtcgcg ttctgccctg
     1681 cctgacgcct gaagtcgcgg acgcgatagg cctcaaaaca ggggaagata agggggaaaa
     1741 agatcctgtc tgtacgtttg ctaaggaact cgctcccggc atcaccagcc agacacagtt
     1801 gtcacaattg cgcctggact tatcggtgcc acagagtcaa ttgattagtc gccctcgcgg
     1861 ctatgttccc cccagcgagc tggataccgg agcatcgctg gcgttcatga attatattgc
     1921 caactattac aacgttgcct attcagggca gaatgctcat agccagcgtt cgctatgggc
     1981 atcatttaat ggtggcatca accttggtgc ctggcaatat cgtcagttat ccaacatgac
     2041 ctgggataat gacaaaggga atcagtggaa caatattcgt agctatttgc aacgcccgct
     2101 gcccgccata aatagccagt taatgatggg gcagcttatc accagcggaa gatttttctc
     2161 tggactcagt tatcacggcg ttagtctcgc gaccgatgaa cgtatgctgc cggactccat
     2221 gcgcggctat gcgccgacta ttcgcggcgt ggccgcaaca aacgccagag tctcggtaat
     2281 gcaaaacggt catgaaatat atcagaccac cgtggctcct ggccctttcg agataaacga
     2341 cctatacccc accagctaca gcggcgatct ggatgtcacc gttacggaag ctaacggcgc
     2401 agtcagtcgt ttcagtgtcc ccttttcagc cgtaccagaa tcgatgcgtc caggaacttc
     2461 ccgttataac gtggaagtag gtaaaacgca ggatagtggt gatgactcga tgtttggtga
     2521 ccttacctgg cagcacggga tgactaatac gctgacattt aacagtggtt cgcgtatcgc
     2581 tgatggctac caggcgctga tgctgggcgg agtctatggc agttcgctgg gggcatttgg
     2641 ggcaaacctc acttggtccc atgcgcgtgt tcccgaaagc gaagcgcaga gtggttggat
     2701 gtcgcaatta acctggagta aaactttcca gcctacttca accaccgtct ccctggcagg
     2761 ttatcgatac tctaccagcg gctatcgtga tctggctgat gtgctgggag agcgtcatgc
     2821 tgccagcaat aaacagtcat gggactccag ccagtggcgt caacagtcgc gcttcgatct
     2881 tacgttaagt cagagccttg cgaattacgg caacctgttt gtgtcaggtt caacacagaa
     2941 ctaccgtggc ggcaagagcc gtgatacaca gcttcagtta ggttacagca atagctttag
     3001 ccatggcatc agtatgaacc tttccgtcgg acgccaaaga atgggcggct ataaagacaa
     3061 ttctgatgat atgcagacgg taacatccct ttcattctca ttcccacttg gcggcaatgg
     3121 acctcgtgta ccaagtctta gcaacagctg gacccattca actgacggta gctcgcaatt
     3181 acaaagctcg ctaaccggaa tgcttgatga agcacagacc accaactaca gcctgaacgt
     3241 catgcgcgat caacaatata agcagacgac gcttagcgga aacatgcaaa aacgtttttc
     3301 acaaactacc gtcggattga acgcatcgaa gggccaggat tactggcagg cttcaggtaa
     3361 cgtacaaggc gcgatggctg tgcatggtgg cggcattact ttcggacctt atctgggtga
     3421 aacgttcgcc ctggtcgaag ctaaaggcgc agaaggtgca aaagtctata actccagtca
     3481 gctggaaatt aatgacagtg gctatgcgct tgttccggca gtaacgccct atcgctacaa
     3541 ccgtatatct ctcgatccac aaggaatgga tggcgatgcc gagttggtcg acagtgaaag
     3601 acaggtagca ccggttgcgg gtgcggcggt gaaagtaatt ttccgtaccc gtcctggtaa
     3661 agcgttgctg attaaatccc gcatggcaga tggttcggaa ctgccaatgg gagccgatgt
     3721 gctggatgag aataatacag tcgtcggtat agccggtcag ggggggcaaa tttacctccg
     3781 cacagaacag acaaaaggcc acttgtcagt tcgctggggt gaaggtgcta acgatagctg
     3841 ccaattgccc tttgatatca gcgggaagga cagcaatagc cctatcatcc gcctgaatga
     3901 aacctgtcag tcttgataca gagaaaaccc gatgtctaaa tcgacttttt tacacattct
     3961 gattagctcc atcatattgg tggcactaat acaatcatcc gcttgggcta actgtacaaa
     4021 cacacagata ggtcaaactg aagatggtcg gacagcgctt attgagttcg gaaaaattaa
     4081 tatgaccgac acctattttg cgccagcagg ttcactcctc gcgacaacgg tcgtacctcc
     4141 cactaactac acatcaggtg gcgcgacagg aagctccgta ttgtgggaat gtgatgcaac
     4201 agatttgcca aacatctatt ttctggtcgc taccaatggc gacgaccgcg tggggggctt
     4261 ttacgatgca ggcgggcctg atggtctgag tgatgtctat gccacctggt tcgcttttgt
     4321 cggtctcaag cagaccatgg cgggcgtgac gcttggtcgt tactggaaga aagtgcccat
     4381 caccagttat gccactcagg gaactaaaat ccagattcgc ttacaggata tccctcctct
     4441 tcatgctgag ctttatcgca tcagtacgct acctgataca tcagcaacaa caagttggtg
     4501 cggtaataat aatacagata gtagtggagt cggattcgca aaaccttccg gtacaatcta
     4561 taactgtgtt cagcccaatg cctatattca gctttccggt accagcggca ttttatttgg
     4621 tcatgatgag cccggcgaag atagttctgt tcattgggat ttctggggtg ctgataatgg
     4681 ttttggttac ggaatgcgtt cggccaatcg actctacaac aatgccacct gcgttgcccg
     4741 cagcgccacg ccgttagtat tgctgccgac aattgcagaa gcacaactga atgcgggcat
     4801 ggaaagtacc ggtaatttta atgtccgcgt cgagtgtagt aactcggttc aatcagggat
     4861 tagcgatact cagacagcat taggaatcca ggtgtctgaa ggtgcatata cagcggcgca
     4921 aaaactgggg attatcaata gcaacggcgg cgtcagcgcc ctggtctctg ataattatga
     4981 cgcagcagag atggcaaagg gcgttgggat ctacatttct aacagtgctc accccgatac
     5041 ggcgatgacg ctggttggtc aaccgggcat cgcgaagtta acccccggag gaaatgcagc
     5101 ggggtggtat cctgtatttg aaggggcaac attagaaggt gcgactcacc ccggatactc
     5161 cagctatagt tactctttta tcgcccggtt gaagaaactg ccaaatcaga cagtcagtgc
     5221 gggaaaagtg cgggcaacgg cttatatatt ggtgaaaatg caatgaaatc cgttttgctg
     5281 ctcttaacca tcttgttcgt acagtcagtg gaagccggca tcatgccggc ttcttcacgg
     5341 attatttatc gcgatggcga tcgggaaaag acactgatgc tggtcaatac caatgactat
     5401 ccggtactgg tgcaaagttg ggtagataat ggtgaaggtt ccccagaagc tgcttctccc
     5461 ccctttgtgg tgctgccacc ggttttccgc ctggctgcga gacgaaccca ggggttgcgg
     5521 attatctata atcatgaccc acttccccag gatcgcgaat ctgcattctg gttaaatctt
     5581 tatgaaatgc cgccagaaac tccccatcaa gatcccacac atttaacgct ggcaatgaat
     5641 acgcaactta aaatctttta caggccaaaa tctgtaacta tgacgcccca agaagctatg
     5701 cgtaaaataa gttggcatct gggtaacgac ggcagcagta gctatattga gcttaataac
     5761 ccaacaccac tgaatatctc tttcacctcc atcagcattg gggctacaaa ggtgcagcaa
     5821 gaaagcgata tgatgataaa acccttcagt caacgacggt actggttagc aagtaataac
     5881 gttagtggtg tggttgcggc gacatatctc gatgataatg gtgcacagat aaaacaacaa
     5941 ttagcgcaat aatgctctga gtggtatgta aactcatcgc gatggttcaa cgctaacgaa
     6001 taaccactat acccacctga agtaccggaa gtggagaaat gacaaaatct ctccactcca
     6061 ttttccagtc aaaacacctc aacgcagcca aaaatcgtta acgaattgaa tatccaccat
     6121 caatgactaa tagttgtcca ttgacataat ctgaagcggc actggcgagg aacaccattg
     6181 cgcccataag atcctgtgcc cttccccagc gttgagccgg tatacgccct tttattaact
     6241 cttttatttt agggttatta taaataatag cggtcatttc actcacataa tatcctgggg
     6301 cgataccatt aatctgaata ttatattccg caaaatcatc ggcatacgct ttggtcagcc
     6361 ccgctaaagc atgtttagtc gccgcatacc ctggcgacca acgtccccca tgaaatgaca
     6421 atacggaaca catattaata attttccctt ttctttgcgg gacaaatata ttcaatgcgg
     6481 cctggctcat atcgaatgca gcattcaggt tgagattaat catcggatcc cagtcgtttc
     6541 gattgaaatc gataggacga ttaatgttac acacgcccgc gttattgacc agaatatcta
     6601 actgaccgaa acgctccaga cacctattaa taatggtgca tgatgcatca ggttttgtta
     6661 tgtctattgt cataaactca acttcaacgc cttcatgagt taaatgcttc aaatatctat
     6721 tatcaggctc tttgatgtca accaacaata gattagcccc tgctttggcc agagccgagc
     6781 ataagcctct ccccagctca cccgcggccc cagttacgat ggccaccttg ttatgtaatg
     6841 aaaaaaaatc caatgaaaaa tcttcaatag aaaagctcat atcacacatt gctccttatg
     6901 agtaagtaat atttaaggga taatttaagt aatttttaga tttcaataaa aaaatataaa
     6961 cattaaaact cagcatgaat aataaacatc
//
LOCUS       DAZFTN010000193         6822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFTN010000193 DAZFTN010000000
VERSION     DAZFTN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            159..1397
                     /locus_tag="ACHKV2_004791"
     CDS             159..1397
                     /locus_tag="ACHKV2_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6942518.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGALKANPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDIYNRTIYLEQRKIMMQWVGDFFDD
                     ARKGVINRSGGMKGLRVVNG"
     gene            1411..1614
                     /locus_tag="ACHKV2_004792"
     CDS             1411..1614
                     /locus_tag="ACHKV2_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942519.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            1674..2255
                     /locus_tag="ACHKV2_004793"
     CDS             1674..2255
                     /locus_tag="ACHKV2_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO6942520.1"
                     /translation="MNKNIAVTGKGDARHVKKICDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCVALEISNSRDALKALDADEKKTVALSYGIRGNPNHSLISESGFYKLIARS
                     RKAVTPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRCKEQYQLSASQKGRE
                     LQACKRKKRELEEEEKRLIREYQPEFYFGNRIQ"
     gene            complement(2376..2555)
                     /locus_tag="ACHKV2_004794"
     CDS             complement(2376..2555)
                     /locus_tag="ACHKV2_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6942521.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKEAE"
     gene            2752..2943
                     /locus_tag="ACHKV2_004795"
     CDS             2752..2943
                     /locus_tag="ACHKV2_004795"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6942522.1"
                     /translation="MRNHTTHPQGRDLHNLNKFTWRFIALSTAQPRVITIEATSEQEA
                     CQQSPDGCVMVFAARIRQG"
     gene            2947..3312
                     /locus_tag="ACHKV2_004796"
     CDS             2947..3312
                     /locus_tag="ACHKV2_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942523.1"
                     /translation="MHNLSISDLNCIQFDEKFTGQLLVHVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPLTNHHDDDLHFTGRMVSHYENGVEVSRERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
     gene            3305..3538
                     /locus_tag="ACHKV2_004797"
     CDS             3305..3538
                     /locus_tag="ACHKV2_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942524.1"
                     /translation="MRDDRFNSLKREFDGAQEDAAGALLSVAGMMKAAYFLINTSGYK
                     SEGEMILSIASDYAEYVAETRYRRKSTEDVSHA"
     gene            3531..3731
                     /locus_tag="ACHKV2_004798"
     CDS             3531..3731
                     /locus_tag="ACHKV2_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942525.1"
                     /translation="MHNHEAHVPVVLNVPDDFTGRVLVYLDKGKVKSQCRLKSNEIVG
                     SPEFFSELCIRAEIKPELLTGK"
     gene            3736..4026
                     /locus_tag="ACHKV2_004799"
     CDS             3736..4026
                     /locus_tag="ACHKV2_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6942526.1"
                     /translation="MKKKNSGFTASGLSRPEIRHGDIYRDTRRGGRVVIRHVTPGNIT
                     YRREAYEYDCVMPRRQFERDFILVENKQQAVARRAATNIKKIRAMLVAGGKK"
     gene            4023..5828
                     /locus_tag="ACHKV2_004800"
     CDS             4023..5828
                     /locus_tag="ACHKV2_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO6942527.1"
                     /translation="MKLAPNLKKQPHDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGDSQLDELADLKIIDDGRYCVRLYKAGHIKPSNINAIGQKLAVAGVRDANYY
                     PEGMHSQKRENWHEYLERERQNLSDGLVIQLPVKKKTEDSAAPLALNQMGASQRGEVL
                     LARYGGELAINADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIKSAVD
                     TMKLSLPVMGNAARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFSPPAEGETLA
                     THAPNFWKWLRRSVAENDRKADRVLAALFMVLANRCDWQLFLEVTGAGGSGKSVMAEI
                     CTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGDKVA
                     IDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDPMLAE
                     KIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLMSSVM
                     CDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYGREYM
                     KRKTKHGLRSNVTLTEDSEDWMPSCASVTNDDGKN"
     gene            6116..6361
                     /locus_tag="ACHKV2_004801"
     CDS             6116..6361
                     /locus_tag="ACHKV2_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942528.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            6358..6780
                     /locus_tag="ACHKV2_004802"
     CDS             6358..6780
                     /locus_tag="ACHKV2_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6942529.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVMAFGRQAEALERHRKGELLSVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RTARLGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         1946 a         1378 c         1830 g         1668 t
ORIGIN      
        1 acatttctta cctgtttgta tgttctggtg tcgattcata gtcttttcaa tgagttgtga
       61 ttttatgagt ttcctctctt tgcttgataa tgagttagtt tatcgcttgt tattggcttg
      121 aatggactac atgacggact aaaaaatgag ggcgatagat gtcggtaaag ccattaaccg
      181 tgactgaagt taaggggatg aaaccacgtg aaaaggacta tgccgtttat gatgggttcg
      241 gtttattgct gaatgtgagt aaagccggtg ggaaagtgtg gcgtttccgt tatagccatc
      301 cgataacgaa gaaacggcag acatacacga taggacgttt tcctgaattc tcactcgcgg
      361 aagcacggga agtacgtgat gaacttcggc gaatgattgc acgtggagtt gatccagtga
      421 cggagaagaa aaatcgtaaa attgagatgt cactaaaaaa tctacagaca tttgaagctg
      481 ttgctaatgc atggttcgct tttaaaaagg gatctgaatt gcggaaacct acgctgtata
      541 atatcgaata tgaagtatac aaatatcttg ttcctttctt tggtaagtac agtatagaaa
      601 aaattacagc accagtagct attaatgctc tggatgccgt atccgataag aatgcgttgc
      661 aaaaaaaatt aatatcaaga ttaaatgaaa ttatgaatta tgctgtaaat tgtggagcat
      721 tgaaagcaaa tccattactt aagataaaga ctgcattcac aggaaagaaa aataaatcat
      781 tagcagcact acctgttgaa agattgcctg aatttctgag ctggtgggat agtgtgcctc
      841 atacctatca aatagctcat aatgcacttt tattccagat attgacaatg gtcaggccag
      901 gtgaggcgat taaagcagag tggtcagaga ttgattttga ttctggcttg tggattatcc
      961 ccgcgcataa aatgaaatgc catcgtgaac atgttgttcc cttgtcatca caggctatta
     1021 gtatcctcag aacaatgcag gaaataaaaa gagggcgtta tgtgtttttt tcctccagaa
     1081 caaaagatgc gcctatgggg aggaatacta tcaagacccc aattgctgcc agtaagttca
     1141 aagggattgt aacgttacat ggttttcgtt caatgtggag tacgctttta aatgaggagg
     1201 gatttaaccc cgatgtaatc gaggctgcat tggcgcataa aagtggtgat aaaataagag
     1261 atatttataa tagaactatt tatctagaac agcgtaagat catgatgcaa tgggtcggtg
     1321 atttttttga tgatgcgaga aaaggggtaa ttaatagatc cggtggtatg aaaggtttaa
     1381 gagtagtaaa tggttgagga ggttcagcaa atgaatacca atgaagatat tttatttact
     1441 aaagacgtaa tgaaaatctt gcgatatgga gcaatgagtg cattcatcaa tttctggaaa
     1501 gatgagaata atggttttcc tcagccgttc agaattggac gacgacatac ctggcaccgt
     1561 agagatgtag aagcatggtt agataaacaa cgagaacaag ccaatcccca ctaataatat
     1621 ctttcatacc ccgcgtgcaa tgcggggttt tttgtatgtg aggtaaaaaa gaaatgaata
     1681 aaaatattgc cgtgacgggc aagggtgacg cacgtcatgt aaaaaaaatc tgtgattttc
     1741 gtgatctggt cgttctgcgc tttgatagtg tgaacgttcg cgtggtgtat ctgaacggcg
     1801 atccgtggtt tgttgcaaag gatgtttgtg ttgctctgga aataagtaac tcacgcgatg
     1861 ctctaaaagc actagatgcg gatgaaaaga aaaccgtcgc tttaagctac ggtattcgag
     1921 gaaatccaaa tcatagcctc atttctgaat ccggttttta caagctgatc gccagaagtc
     1981 ggaaagctgt tactcctggc acgttcgccc atcgtttcag taactgggta ttcaggaatg
     2041 tgataccagg aatcagaaaa acgggggctt atggtatccc gtggggcgca ttacaggact
     2101 tttctcgctg taaagagcaa tatcagttaa gtgccagtca gaaggggagg gagctacagg
     2161 catgtaagcg caaaaagcgt gagctggagg aagaagaaaa aaggctgata cgtgaatatc
     2221 agcctgagtt ttactttggc aaccgcattc agtgacaaaa caaaggcgac ccggatcaga
     2281 ggtcgccaat gggaaaacac taaacataag cccaaacatc atagcgattt gttggctggt
     2341 gggcaatgtg atcagtgtgc tggcttgctg gtggactatt ccgcttcttt ggccttgcgg
     2401 cgctggcggc gtttgatctc gcctcgcatg gctgtaacga taaattgcgc agtgctctcg
     2461 cctgtttctt ttacttgctc catagcctca acaacttcat gcggggcgcg agcctgtaat
     2521 ttctgtgatt tgttattgat atggtctctt tccatttctg gttccctttg tgattactgg
     2581 aatccagtat acacgaaaaa gaccaaaaca aaacgcttga agtggattcc acttagtgat
     2641 tatactggaa tccagttgag tgtttatcac tcaatgcaaa cgaggctgaa aaggattgcc
     2701 gtcctttcgc agcctctgac caccaacgat agcaacagta tcgaggtagc tatgagaaat
     2761 cataccacac acccgcaagg gcgggacttg cacaacctga ataaattcac ctggcgtttt
     2821 atcgccctga gcacggcaca accgcgcgtg attaccattg aggccaccag cgaacaggaa
     2881 gcatgccagc aatctcctga tggctgcgtg atggtattcg ctgcccgtat tcgtcagggg
     2941 taagtcatgc ataacctgtc aatttctgac cttaactgca ttcagtttga cgagaaattt
     3001 accgggcagc tactggtcca tgtggagaac gggcgcatag tgcgtaatta ccacctgccg
     3061 gatggtgcaa ttgccggaag cgttgaagca ttgctggaac tggcagaacg tgcgcgactg
     3121 attaagccgt taacgaacca tcacgatgat gatctgcatt ttaccggacg catggtgagt
     3181 cactacgaaa acggcgtcga ggtatcccgc gaacggctgc gtgatgattg ctgtttcggc
     3241 acactgccgg aatttatcga gttgctgacc agttgcgggt atcaggtcat tcaggggggt
     3301 aaacatgcgt gatgatcgtt ttaattccct gaaacgggaa tttgatggcg cacaggaaga
     3361 tgcagcgggc gcattgttga gcgttgctgg catgatgaaa gctgcatatt ttcttatcaa
     3421 taccagtggc tacaagtcag agggtgaaat gattcttagt attgcgtcag actatgcgga
     3481 atatgtggca gagacgcgtt acagaagaaa atccacggag gatgtaagcc atgcataatc
     3541 atgaagcgca tgtacccgta gtgcttaatg tgccagatga tttcaccgga cgcgtactgg
     3601 tttacctgga taaagggaaa gtgaaatcac aatgccgact gaaaagtaat gagattgttg
     3661 gttctcctga atttttttct gaactttgta ttcgtgcgga aataaaaccg gaactgctga
     3721 caggaaaata aaaccatgaa aaagaaaaat tctggcttta ctgccagcgg tctctctcgg
     3781 cctgaaatca gacacggaga tatttaccgc gacaccagac gtgggggacg agtggttatt
     3841 cgtcacgtta cgccaggcaa tatcacctac cgccgtgagg cttacgaata tgactgcgta
     3901 atgccgcgcc gtcagtttga gcgtgatttt attctggttg aaaacaaaca acaggcggtg
     3961 gcgagacgtg cagccacgaa tattaaaaaa atccgggcaa tgttggttgc agggggtaag
     4021 aaatgaaact ggcaccgaac ttaaaaaaac agccacacga caaaatgacc gaagtcatta
     4081 tttttgcggg tagtgatgcc tgggcgcacg cgaaacaatg gcaggaacag gacgggcgac
     4141 ttgctggcga caacgtgcca cctgtatggc ttggagacag ccagcttgac gaactggcag
     4201 acctgaaaat tatcgacgat ggtcgctatt gtgtccggct gtacaaggca ggccacatca
     4261 agccgtcaaa tattaatgcc atcgggcaaa agctggcggt ggcaggtgta cgggatgcga
     4321 attattaccc tgagggaatg cacagccaga agcgggagaa ctggcacgaa tatctggagc
     4381 gtgagcgcca gaatctttct gatggtctgg ttattcagct tcctgttaag aaaaaaacag
     4441 aagacagcgc cgcaccactg gcgcttaacc agatgggagc aagccagcgc ggcgaagttc
     4501 tcctggcacg ttatggcggt gaactggcaa tcaatgcgga ctctgacacc gttcaccact
     4561 acaacggcgt tgtatgggag ccagtacagg ataaagagtt acagcgggct atggcgcaga
     4621 tttttattga tgcggagatc agttattcgc agaacgccat caaatcggcg gtcgatacca
     4681 tgaagttaag tttgcccgta atggggaatg ccgcccgtaa cctgattgga ttcagtaacg
     4741 gtgtttttga tacccggaca ggcgattttc gggagcatga caaaaacgac tggctgttaa
     4801 ttgccagtga attaccgttc agcccgccag cagaggggga aacgctggca acgcatgcgc
     4861 cgaatttctg gaaatggttg cgccgttcgg tggcagagaa tgaccgtaaa gcggatcgcg
     4921 tactggcggc gttattcatg gtgctggcga accgatgcga ctggcagtta tttcttgagg
     4981 taacgggggc gggtggaagc ggtaaaagcg taatggcaga gatttgcacc atgctggcgg
     5041 gtaaggccaa cacggtatcg gcaagcatga aggcgctgga agatgccagg gaacgcgcgt
     5101 tagtggttgg cttttcgctg attatcatgc cggatatgac ccgctacgct ggtgatggcg
     5161 caggaattaa ggccattaca ggcggtgaca aggtggcaat cgacccgaag cataaagccc
     5221 cctactcaac acgcattcag gcggtcgtgt tggcggtgaa caataacgcc atgtcattca
     5281 gtgaccgcag cggggggatc tcacgtcgtc gggtgatatt caatttttcg gaggttgtac
     5341 cggaaaacga acgcgacccc atgctggcag aaaaaataga aggagagctg gcggttgtga
     5401 ttcgtcacct gcttactcgt ttttctgacc aggacgaagc taaacgcctg ctgtatgagc
     5461 agcagaaatc agaagaagct ctggtgataa aacgcgaggg cgattcgctg gtggacttct
     5521 gcggctatct catgtcgtcg gtaatgtgtg atggtctgtt agtgggtaat gccgaaatta
     5581 ttccgttcag tccgcgcagg tatctttatc acgcctatct ggcatatatg agggcacatg
     5641 gattcggtaa acctgtaaca ctgacgcgct tcggtaaaga tatgccgggg gcaatggcgg
     5701 aatatggcag ggagtatatg aaacggaaaa cgaagcacgg tttgcgttca aatgtgaccc
     5761 tgacagagga ttcagaagac tggatgccat catgtgcatc ggtcacaaat gacgatggaa
     5821 aaaattaaac ttatggaata actgttcacc actgttcacc ctgcaataaa tgtcttttat
     5881 atcagtatat tatagggtga gcagttattt atgaactgtt caccaaacta ttcactgttc
     5941 acctttttga ttctttattg agctttaagg gtgaacagtg gtgaacagtt ggtgaatagt
     6001 ttttgtgaaa ctgttcaccc cttaacatta tgaataaaaa gggaaaattg caaaaggtga
     6061 acaggtgaag ggttgaaatg caaaaatttt attttattgc tgtgaggtaa agcctgtgac
     6121 aacgaagcac gccaaaaaac cacaatctca cgcccttgat ttgacagaac actggctgag
     6181 ggtggcgata aaaatcatcg accgcaacgc cggggaagga tacgcgaaag cacatcccga
     6241 actgataagc gcattcatga ccacgacggc ggcaaacttt gccacgctga cagaacggga
     6301 gattgccgaa gcggaacagg taacaaccat caacgttaaa accggagagg tggaatcatg
     6361 acagcacaga tagcagctta cgggcggctg gtggacgacc cgcaggtaaa acagaccagc
     6421 aagggcacac caatgacgct ggcacgtatg gcggtatcgt tgccatgtag tcaggcgcag
     6481 gatgggcagg ctacgttatg gctatcggtg atggcatttg gcagacaggc cgaagcactg
     6541 gaaaggcacc gcaagggtga actcctgagc gtggcgggta acatgcagat cagccagtgg
     6601 actgggcaga acggagaaac gcggcagggc tggcaggtta tcgcagacag tgtaatcagt
     6661 gcgcgaacgg cgcgactggg cggcaaaaaa ggtcaacagg gccaggctac tgacgcgctg
     6721 aacagggcaa aacaacaggc ggggaatgat gatccgtacg gggataacat accgttttaa
     6781 gcgacgagtg acagaagccg gagcaatccg gcttttttgc gg
//
LOCUS       DAZFTN010000194         6678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFTN010000194 DAZFTN010000000
VERSION     DAZFTN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..1712
                     /gene="mppA"
                     /locus_tag="ACHKV2_004803"
     CDS             99..1712
                     /gene="mppA"
                     /locus_tag="ACHKV2_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO6942530.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1763..2794)
                     /gene="ynaI"
                     /locus_tag="ACHKV2_004804"
     CDS             complement(1763..2794)
                     /gene="ynaI"
                     /locus_tag="ACHKV2_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO6942531.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3038..3295
                     /locus_tag="ACHKV2_004805"
     CDS             3038..3295
                     /locus_tag="ACHKV2_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO6942532.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3345..4295)
                     /gene="uspE"
                     /locus_tag="ACHKV2_004806"
     CDS             complement(3345..4295)
                     /gene="uspE"
                     /locus_tag="ACHKV2_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO6942533.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4447..5199)
                     /gene="fnr"
                     /locus_tag="ACHKV2_004807"
     CDS             complement(4447..5199)
                     /gene="fnr"
                     /locus_tag="ACHKV2_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO6942534.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(5394..5909)
                     /gene="ogt"
                     /locus_tag="ACHKV2_004808"
     CDS             complement(5394..5909)
                     /gene="ogt"
                     /locus_tag="ACHKV2_004808"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO6942535.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(5920..>6678)
                     /locus_tag="ACHKV2_004809"
     CDS             complement(5920..>6678)
                     /locus_tag="ACHKV2_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFO6942536.1"
                     /translation="SQRFGLRIAGVVSLLFIAAIALMVIPENGILRDPINHTVMPSPF
                     IKGIVPLIILFFFVVSLAYGIATRTIRRQADLPHLMIEPMKEMAGFIVMVFPLAQFVA
                     MFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSILAPIF
                     VPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGTYYSLV
                     LPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1848 a         1558 c         1602 g         1670 t
ORIGIN      
        1 atgcttcctg tgtcactttc tgatggaagt tgtttgcatt tctttaaggc gaaacaaata
       61 attacgcatc aattttaatg tcggttagag ggaaacttat gaagcactct gtttcagtca
      121 cgtgttgtgc gctgttggtc agcagcattt ctctttcgta tgctgcagaa gttccgagcg
      181 gcacagtact ggcagagaag caggagctgg tgcgccacat taaagatgag cctgcgtcgc
      241 tggatcccgc taaagccgtg ggcctgccag agattcaggt cattcgcgat ctgtttgaag
      301 gactggtgaa tcagaacgag aaaggggaga ttgtccccgg cgttgcgact cagtggaaaa
      361 gtaatgacaa ccgtatctgg acttttaccc tgcgcgataa cgcaaaatgg gcggatggca
      421 caccggtaac ggcgcaagat tttgtctaca gctggcagcg tctggtggac ccgaaaacgc
      481 tatcgccatt tgcatggttt gccgcgctgg cgggaatcaa caacgcacag gcgattattg
      541 atggtaaagc tacacctgac cagcttggcg tcaccgcagt tgatgtccat actttgaaaa
      601 ttcagcttga taaaccgttg ccgtggtttg tgaatttaac cgctaacttt gccttcttcc
      661 cggtgcaaaa agccaacgta gaaagcggta aagagtggac gaaacccgga aatctgatcg
      721 gcaatggcgc ttatgttctt aaagagcgcg tagtcaatga aaaactggtc gtggtaccga
      781 atacccatta ttgggataac gccaaaacgg tactgcaaaa agtgaccttc ctgccaatta
      841 atcaggaatc cgcagccact aagcgttacc tcgcggggga tattgatatc accgaatcct
      901 tcccgaaaaa tatgtatcag aagctgttga aggatattcc ggggcaggtt tatacgccgc
      961 cgcagctcgg gacctattat tatgcgttta acacgcaaaa agggccgacg gcagatcagc
     1021 gcgttcgtct ggcattaagt atgacgatag atcgccgcct gatgaccgaa aaagtattag
     1081 ggacgggcga aaagccagcg tggcatttta caccagatgt taccgccgga tttacgccgg
     1141 aaccttcgcc gtttgaacaa atgagtcagg aagaactgaa tgcgcaggca aaaactttgt
     1201 tgagcgcagc tggttatggt ccgcaaaaac cgctgaagct gacgcttttg tataacactt
     1261 cagaaaacca tcaaaaaatt gcgattgctg tagcatcgat gtggaaaaag aaccttggcg
     1321 tagatgttaa attgcaaaat caggaatgga aaacctatat cgatagccgt aacaccggca
     1381 attttgatgt tatccgcgcc tcatgggtgg gggattataa tgaaccctcc actttcctga
     1441 cattattaac gtcaacgcat tcaggaaata tttcacgctt taacaatccg gcatacgaca
     1501 aagttctggc ccaggcatcg acggaaaata ccgttaaagc gcgtaatgcc gattacaacg
     1561 cggcagaaaa aatcctcatg gagcaagcac cgattgcacc aatttatcaa tataccaatg
     1621 gacgattaat caaaccgtgg ctgaaaggtt atcccattaa taaccctgag gatgtggcgt
     1681 acagtcggac tatgtatatt gtgaagcatt gatgtgatgg gaactggcgt tacccttgtg
     1741 gataacgcca gtgatgtctg atttagcgac cctgttccgg cggtgtaatg ttatccatat
     1801 acagcgtctg gctggggaag gcaaagtccg cgccgtgtga ctgtacaata tcgataatct
     1861 tcaaataaac gtcttgctgt gcagcaagcc actcagccca taccgtggtt ttggtaaagc
     1921 aataaaccat aatattcaat gacgagtcag caaactggtt gaaataaacc agtaaggttt
     1981 gtcgctggtc gatggccggg tgatttttca gcatctcacg tacggcttcg acgataacgc
     2041 ccacttttgc cgcatcctca taacgtaaac caatggtcgt ggtaatgcgg cggttggtca
     2101 ttcgtcctgg gttttctacg ctgatcgatg aaaacagcga gttcggtacg tacaatggac
     2161 gattatcaaa ggtcgtaatt ttggtaattc gccagccaat ttccgctact gtaccttcga
     2221 tatttctgtc cggtgaacgg atccagtcgc caatactgaa aggacggtcg aaatagagca
     2281 tgatcccgga aaagaaatta ctcagaatat ctttgccagc cataccgaca gccagaccac
     2341 caataccacc aaaggtcagc aagccagaaa ggctcatgcc gaaatgttcg ccataaagca
     2401 gaacaagcac cacaataatg gtgattttga taatgcgcga cataatccgc gcactggtga
     2461 tatcgcgacc ctttttaatc tgctgttttt caaactgatt aatcagcaaa aatagcttaa
     2521 tcgtcagaat aaccgcaatc agggacgtac agataaaatc gataacgcct ggagtgataa
     2581 atttgagttt atagttttct ataacataat taataatgct accaacagca ctgataatta
     2641 tggtgtagat taaaaattgc accgcatgga ataaaaagcc ttttctttta cgatttccgc
     2701 ggcgaaacca aaagctcatc agaatcaatg ctgcgcagct accgaaaata atgaccagat
     2761 taagcgcatt atttgtaaac agttcagcga tcattgtttt atcaggctcc tccagataat
     2821 tgtcgtcatg ccggaaaccc ctggcggggc tattttaccg cgacaattca ttcagatcat
     2881 caatagtcag ggaaggaagt agcaacatta gctaactaat caatgcgttg attgtaaatc
     2941 cagctaagag gtgaggtttt cagagcagac aatggtgaaa tgtcatggta ttgttacgtt
     3001 taggtaacaa gaaatttgtc tgcacaagga ttacatcatg attatggcga aactgaagtc
     3061 agcgaaaggg aagaaatttc tctttggttt gttggcggtt ttcattattg cggcgtcggt
     3121 tgtgactcgc gcgactatcg gcggcgttat agaacagtac aatattccgc tgtcggagtg
     3181 gacgacatca atgtatgtga ttcagtcatc gatgattttt gtttatagcc tggtctttac
     3241 tgtgttgctg gcaatcccgt tgggaattta tttccttggc ggcgaagagc agtaagtaaa
     3301 aaataggccc gataactcgg gccttgtcag ttattgagga gtcgttaatc gtcttcttcg
     3361 tcatccagtt caacgggtgt ctgatactgg tcaggtttaa tgaccaacag atcgcagcga
     3421 agatgatcaa tcacctgttc cgccgtgttg ccgaggaatg ctgctgaaat accggtgcgt
     3481 cctaccgtgc ccagaaccac aatccccgcc tgtaagtgct ccgccaaatc aggaatcacc
     3541 tcttctggca gacctttttc tacgtgcgtc atgttttcat taatgccgaa tttctgccgc
     3601 agggctttca ttgccagcaa atgttgccca cgaatggcat cgttataaac gctcggatca
     3661 aattccggca gttcaatcgc gatattaatt ggcgttaccg gataagcgcc aaccagatga
     3721 acttcggtat ggttgacttg ttctgccagt tcgatcgtct ctttgaccag tttttcattg
     3781 agcgcattat gatacggctc ttcactggcg agattcaccg ccaccagcgc cttacctcct
     3841 tccggccacg gctggtcttt caccatccac accgggcttg ggcatttgcg taacaggtgc
     3901 cagtccgttg gcgtaaaaat caccgcttcc agacggtcat gttggtgcgc catttttagc
     3961 accaaatcgt gcccgccgct gatcacttcc tgaatgatgg cttcgaaagg acggttatgc
     4021 cagaccactt taatttcaat gggaacgcca gcattgagat aatattttgc ctgctcgtgg
     4081 atccaggctg tacgctggct gatgacgccc tgacgcatag cggtacgttc gtccggggag
     4141 agcagggtgg tcatttcgta tgagaagtca tagatcggca aaaaggcttt aattttgcca
     4201 ccaatccgtt gatgtaaata aacagctcgc cgcaatgctg gttggtcgtc ctggttagga
     4261 tcgataacaa cgagcatgtt ctgatacata gccatacagg gtctccttac aacaactgtc
     4321 aacgcagttt gtaattaaaa gattaaccca tatctggtga atgaaacagt gatgaacctt
     4381 ctgccagatc aataaatcag aaaaatttaa tgatatgaca gaaggatagt gagttatgcg
     4441 gaaaaatcag gcaacgttac gcgtatgacc agcaagctgg gccagcgcat cgttattttc
     4501 gatggtgatg tatttacctt tgactgccag cataccgctt ttctggaagc gacccagcag
     4561 acggctgatg gtttctaccg tcaggcccag atagttaccg atatcgccac gagtcatcgt
     4621 caggcggaat tcacgagggg agaagccgcg ttgggcaaaa cgacgggaca ggttgtagat
     4681 gaatgcagcc agacgttcct cggcattttt ctttgacaac agcaggatca tgtcctgatc
     4741 gcctttgatt tcaccgctca tcagacgcat catctgctga cgcagattcg gcattttacc
     4801 ggacaaatcg tccagcgttt cgaacgggat ttcacatacc atcgaggttt ccagcgcctg
     4861 cgcgaagctc gggtgatggc cgctgccgat ggcgtcaaat cccaccaggt cgcctgctaa
     4921 atggaaacca gtgatttgct cgtcgccttg ctcagtgatg gtataacttt taatcgtacc
     4981 ggagcggatg gcataaagcg atttaagttc atcaccagcc ttaaacagcg tctggccttt
     5041 ctgaataggc ttcttccgct caatgatatt atcaagctga tcaagctcat gttcgttgag
     5101 tgtgaacggg atgcaaagct ggctgatgct gcaatcctgg caatggatag cacaaccgcc
     5161 agactgaatg cgccgtataa ttcgcttttc cgggatcata ggtctgctca agccgtaatt
     5221 gatatttgtc aattttaaca tctttttagg gagcaagtaa gtctaagcaa accttaacag
     5281 cagagaattc cgatattaga tgtaaatata tgtctatcta tttgaaaacc cttaagttgt
     5341 taagggtaac tttacataaa agtgtgaaca agctggcaca aattgtttaa tgtttacagc
     5401 aaaagataac cttcgtggcg caataaccac tcttttcgct gaactccgcc tgcatatccg
     5461 gtcatggtgc cgtttcggcc aataacccga tggcaaggta cgacgatgct gatgggattc
     5521 gatccgtttg ccgcaccaac ggcacgcgcc gcgccaggac ggcccaattg ctcagccagt
     5581 tcgccgtaat gcattacctg cccgcagggg atagtgcgta gtgttttcca gacttcgcgc
     5641 tgaaatggcg tcccccccgt agcagtggga agcgtatcaa taatgctaag attaccggca
     5701 aaatattcac gaagcttgtc gcttaaaccg cctggattgg tggcagaaat gcgctcatag
     5761 ccttctttgc gataatggat gtccagcagc tgcaccatgc gttcgctgta ctcttcccat
     5821 tcaaccgccc gcaggcgaaa ttgctcatcg caaatcaccc acagtggacc cagtggtgtg
     5881 gcaatttttt cttcaagtaa tctcagcatc cgttctctct taagacaaac gtgggtaaat
     5941 acccggacct atcggcaggc ccacaagata ccacgccagc aacatcagca gccataccac
     6001 caaaaagata agcggatagg gcaagactaa cgaatagtaa gtacccagtt tcgcgtctgg
     6061 tttgtagcgt tgcaggaatc caagaaacag tggaacaaaa ggagatactg gcgctaaagg
     6121 caatacggat gagtcggcaa tacgaaagag gatttgcgca aatgccgggt gaaagccaag
     6181 tagcataaac attggtacga aaatgggggc cagaatcgac cagattgcgg aaccgctggc
     6241 gataaacatg cataagaaag aggaaagcaa cgccagaccg acaaacgccg ggatgccgct
     6301 aagccctgaa ctttccagga tatcggtcag ccccacggcg atgaatttcc ccatgttgct
     6361 ccagttaaac atggcgacaa actgggcgag gggaaaaacc atcacgataa atcccgccat
     6421 ctctttcatc ggttcaatca ttaaatgcgg taaatccgcc tgacgtcgaa ttgtgcgggt
     6481 agcgatgcca taagccagcg agacaacaaa gaaaaaaaga atgatcagtg gcacgatacc
     6541 tttaataaag ggtgatggca tcacggtgtg attaatcgga tcgcgcaata tcccgttttc
     6601 cgggatcacc atcagcgcaa tcgcagcaat aaaaagtagc gatacgacac ctgctatgcg
     6661 taaaccaaaa cgctgact
//
LOCUS       DAZFTN010000195         6585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFTN010000195 DAZFTN010000000
VERSION     DAZFTN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(323..2356)
                     /gene="betT"
                     /locus_tag="ACHKV2_004810"
     CDS             complement(323..2356)
                     /gene="betT"
                     /locus_tag="ACHKV2_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO6942537.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2485..3072
                     /gene="betI"
                     /locus_tag="ACHKV2_004811"
     CDS             2485..3072
                     /gene="betI"
                     /locus_tag="ACHKV2_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO6942538.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3086..4558
                     /gene="betB"
                     /locus_tag="ACHKV2_004812"
     CDS             3086..4558
                     /gene="betB"
                     /locus_tag="ACHKV2_004812"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO6942539.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4572..6260
                     /gene="betA"
                     /locus_tag="ACHKV2_004813"
     CDS             4572..6260
                     /gene="betA"
                     /locus_tag="ACHKV2_004813"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO6942540.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1651 a         1807 c         1785 g         1342 t
ORIGIN      
        1 atccgggaac tcaaatcaac tcaaaatata aacaaaagca ggagaaatac atttaatata
       61 cagccggtaa aagtggtaac tcttattagg tatccaaata taagaatcgt cttattaata
      121 aatatataac cctataggac tgaatccatt cagctaatac aaaataaaat cgttaattaa
      181 taaaatataa aatcatgaag tgatacaaga ggaatttcag gatatgacat gacaatcata
      241 tataatatat gcataatttt ttacagcgag tcaatgattc ccgcgccata tgcagacatg
      301 gcgcggtttt atgcaataac aatcacgcgt ccgggaacat cacgctatgg cccggcgctt
      361 cacgatggag atgaataaag ttaaggtgcc gctcgtactg gtcaagaata tcggtgatca
      421 cctgctcttt gctgtagtcc atcaggtcgt tgccctggct gccttctaac aggaaggttt
      481 ccagccggta gtaggtcgat ttcccgctac gtgcgcggta ggtaaagccc ggcaccgagt
      541 attgctgcgg ccaaatctga tagacaaagt tttgctcttc gcccatatgc accaacaaat
      601 ccagatgccc caactgttgt ccctcttccg gcggcaggct ttttagctcc acgtacgcgc
      661 cgcgcaaccg caactcctgc gccacttctt ccattgccgg gtaacagacc gtctccatca
      721 tctgtttagt gtaacgcgtg cccggataat tcatcaggcg cgagagacgt tttttccagc
      781 tcaggcgatc ctgaagcccc agcggtcgcg gtgcggtatc gcggttggca ctttcacggc
      841 ggtaatcttc taccttcaga gatttataca accccgccat cacgaagaag atcacaaagc
      901 tgaacggcag ccccataatc accgtggtgt tttgcagcgc ggatatcccg ttggtcatca
      961 gcatgccgag cgtcagcagg ccaatcgcca ccgaccagaa gacgcgcagc cagccggggg
     1021 cgtcgctgtt gatatcttta agctgcgagg tgaaattccc cagcaccagc gccccggagt
     1081 ccgccgaggt cacataaaac agcaggccag taatggtggc gacggaggcg ctaaaggtaa
     1141 acgccggata ctgcgccagc aggctgtaga agccgcgctc cggatggacc atcgcttcct
     1201 cggcaaatgc cgcgccgccg tggatgattt catacagcgc gctattgccg aacaccgaga
     1261 gccataacag cgtgaaggta aacggaataa tcaacgtgcc cagcacgaac tggcgaatgg
     1321 tacgcccacg cgagatacgc gccaggaaca agccgacaaa cggcgaccat gccacccacc
     1381 atgcccagaa gaagagcgtc cagttattca tccactcaac cgggcggtcg aaggcaaaac
     1441 tgttgagcgt catgcccata aagcgattca catagtcgcc aacattcagc accagtgcat
     1501 taagcaggaa cgaagtgtcg cccataaaca acacgaacag gatcaatccc agcgccagcg
     1561 cgacattaag ctccgataac acgcgaatgc ccttatcgac accggatgtc accgagatag
     1621 tggcgattat caccgacaag gcgatcagtg ccgcttttgc cgccatcgaa tcgggaatat
     1681 caaacagtac gctcaagcca tagttaagct gcaccacacc gataccgagc gtagtggcaa
     1741 taccgaagat agtgccgatc accgctgcaa tatccactga gtgacctatc ggcccgttaa
     1801 tccgtttacc gaagatcggg tacagcgccg agcggatggt gagcggcaaa ttataacgat
     1861 agctaaagta tccgagcgcc atgcccatca gcgcatacat cgaccagccg gttaagccgt
     1921 agtgaaacag cgtccagacc atcgcctgac gcgcggcctc aatcgtctgt cccgcgcctt
     1981 ccggcggctg catatactgc gttaccggtt cggctacgga aaagaacatc agatcgatac
     2041 cgatcccggc agcaaacagc atcgccgccc aactcagcag gctgaattcc ggtttggatt
     2101 gttctggccc gagcttcacc gaaccaaaac gcgaacaagc gatacagacc acaaagacaa
     2161 tatagagcgt tgccgccagc agatagtacc aaccgaaggt tttagaaacc cagtccagcg
     2221 tgcggccaat ccacagggcc gagaagtcgc gaaacaggat cgttgtcagg gaaaacaaca
     2281 aaatcagtcc ggcggaggtg taaaacacca ccggattgat tttgtccttt tccctgctgt
     2341 gtgaaaggtc tgtcatccag tatccccact gttattgtta ccatttaaaa tcaaattcgt
     2401 aacaattaag acacatttta tattgaacgt ccaatcaata accgctttaa tagataaaca
     2461 acgctgatga atggagtggc aaaaatgccc aaattgggga tgcagtcgat ccggcgcaga
     2521 caactgatcg acgccacact ggaagcaata aatgaagtgg gcatgcacga tgcaacgatc
     2581 gcgcagatcg cccgccgtgc aggcgtttct acggggatca tcagccacta tttcagggac
     2641 aaaaatggtc tgctggaagc aaccatgcgc gatatcacca gtcagctgcg tgacgcggtt
     2701 ttgaatcgat tacatgcact tccgcagggc agtgcagagc agcgattaca ggcgattgtt
     2761 ggcggaaact tcgatgaaac gcaggtgagc agtgcggcga tgaaagcctg gctggcgttc
     2821 tgggccagca gtatgcatca gccgatgctc tatcgtttac agcaggtcag cagccgccgc
     2881 ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc cgcgccacca ggcacaggaa
     2941 gcgggctacg gccttgccgc gctgattgac ggattatggc tgcgcgcggc actgagcggc
     3001 aaaccgctgg ataaacctct cgcacactcg ctgacccgtc actttattac tcagcattta
     3061 cccaccgatt aaccgaggag acgtgatgtc ccgaatggca gaacagcagc tttatataca
     3121 tggtggttat acctccgcca ccagcggtcg caccttcgag accattaacc cggccaacgg
     3181 taacgtgctg gcgaccgtgc aggccgccgg gcgcgaggat gtcgatcgcg ccgtgaagag
     3241 tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct atggaacgct cgcgtattct
     3301 gcgtcgggcc gtcgatattc tgcgcgagcg caatgatgaa ctcgccaaac tggaaaccct
     3361 cgacaccgga aaagcgtatt cggaaacctc aaccgtcgat atcgttaccg gtgcggacgt
     3421 gctggagtac tacgccgggc tgatcccgtc gctggaaggc agccagatcc cgttgcgtga
     3481 gacatcattt gtctacactc gccgcgaacc gctgggcgtg gtggcaggga ttggcgcatg
     3541 gaactacccg atccagattg ccctgtggaa atccgccccg gcgctggcgg cgggtaatgc
     3601 gatgattttc aaaccgagcg aagtcactcc gcttaccgcg ttaaagctgg ctgaaattta
     3661 cagcgaagcg ggcctgccgg acggtgtatt taacgtgttg ccgggcgtgg gcgcggagac
     3721 cgggcaatat ctgaccgagc atccgggcat tgccaaagtg tcgtttaccg gcggcgtcgc
     3781 cagcggcaaa aaagtgatgg ctaactcggc agcctcttcc ctgaaagaag tgaccatgga
     3841 actgggcggt aaatcaccgc tgatcgtttt cgacgatgcg gatctcgatc tcgccgccga
     3901 tatcgccatg atggcaaact tcttcagctc cggtcaggtg tgtaccaatg gcacccgcgt
     3961 cttcgttccg gcgaaatgca acgccgcatt tgagcagaaa attctggcgc gcgttgagcg
     4021 cattcgcgcg ggcgacgttt tcgatccgca aactaacttc ggcccgctgg tcagcttccc
     4081 gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa gaggaaggcg cgcgcgtact
     4141 gtgcggcggc gatgtactga aaggcgatgg cctcgataac ggcgcatggg ttgcaccgac
     4201 cgtgttcacc gattgcagcg acgagatgac catcgtgcgt gaagagatct tcgggccggt
     4261 gatgtccatt ctgacctacg agtcggaaga cgaagtcatt cgccgcgcca atgataccga
     4321 ctacggcctg gcggcgggta tcgtgacggc ggacctgaac cgcgcgcatc gcgtcattca
     4381 tcagctggaa gcgggtattt gctggatcaa cacctggggc gaatccccgg cagagatgcc
     4441 cgttggcggc tacaaacact ccggtattgg ccgcgagaac ggcgtgatga cgctccagag
     4501 ttacacccag gtgaagtcca tccaggttga gatggctaaa ttccagtcca tattctaacc
     4561 aggaggttta tttgcaattt gactacatca ttattggtgc cggctcagcc ggcaacgttc
     4621 tcgctacccg tctgactgaa gatccgaata cctccgtgtt gctgcttgaa gcgggcggcc
     4681 cggactatcg ctttgacttc cgcacccaga tgcccgctgc cctggcattc ccgctacagg
     4741 gtaaacgcta caactgggct tacgagacgg aacctgaacc gtttatgaat aaccgtcgca
     4801 tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct gatcaacggc atgtgctata
     4861 tccgtggcaa cgcgatggat ctcgacaact gggcaaaaga acccggtctg gagaactgga
     4921 gttatctcga ttgcctgccc tactaccgca aggccgagac ccgcgacgtg ggcgagaacg
     4981 actaccacgg cggcgacggc ccggtgagcg tcaccacctc caaacccggc gtcaatccgc
     5041 tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta cccgcgcacg gacgatctca
     5101 acggttatca gcaggaaggt ttcggcccga tggatcgcac cgtcacgccg cagggccgcc
     5161 gcgccagcac cgcgcgcggt tatctcgatc aggccaaatc gcgcccaaac ctaaccattc
     5221 gtactcacgc catgaccgat cacattattt ttgactgtaa acgcgcggtg ggcgtcgagt
     5281 ggctggaagg cgacagcacc attccgaccc gcgcgacggc gaacaaagaa gtgctgttat
     5341 gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc tggcgtcggc aacgctgaac
     5401 tgttggccga gtttgatatt ccgctggtgc atgatttacc cggcgtcggt gaaaatcttc
     5461 aggatcatct ggagatgtat ctgcaatatg agtgcaaaga accggtttcc ctctaccctg
     5521 ccctgcagtg gtggaatcag ccgaaaatcg gtgcggagtg gctgtttggc ggcaccggcg
     5581 ttggtgccag caaccacttt gaagcaggcg gatttattcg cagccgagag gaatttgcgt
     5641 ggccgaatat tcagtatcac ttcctgccgg tagcgattaa ctataacggc tcgaatgcag
     5701 tgaaagagca cggcttccag tgccacgtcg gctcgatgcg ctcgccaagc cgtgggcatg
     5761 tgcggattaa atcccgcgac ccgcaccagc atccggcaat tctgtttaac tacatgtcgc
     5821 acgaacagga ctggcaggaa ttccgcgacg caattcgcat cacccgcgag atcatgcatc
     5881 aacccgcgct ggatcagtat cgtggccgcg aaatcagccc cggcacggaa tgtcagacgg
     5941 atgagcagct cgatgagttt gtgcgtaacc acgccgaaac cgccttccat ccgtgcggta
     6001 cctgcaaaat gggctacgac gagatgtccg tggttgacgg cgaaggccgc gtgcatgggc
     6061 tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca gattatcacc gggaatttga
     6121 acgccacgac gattatgatt ggcgagaaaa tggcggatat gattcgcggg aaggaagcgt
     6181 tgccgaggag cacggcggga tattttgtgg caaatgggat gccagtaaga gcgaaaaaaa
     6241 tgagtcgtga tttgaactga ctcaggaacc gatcagattg agagttaccg ttccaggtat
     6301 ggggacagaa tcgttatata aaacataggg gtaactctca tgcttcatag tgacaacctc
     6361 gccagcaaat tctaagctga ctcgttcaat ctcgctgatg aaggtactat gaatgcactg
     6421 ataaaccgca ggtgaaatgc cactcagctt aagaattacg ccgctagtgt tcctggctat
     6481 ggtgacttgt tcactcccgc tcacggttag catatacgaa aagggttctt gtaacgagga
     6541 caaaaataag tcagaccaaa acaacgtgga gctttaaaca caatt
//
LOCUS       DAZFTN010000196         6505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFTN010000196 DAZFTN010000000
VERSION     DAZFTN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHKV2_004814"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHKV2_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO6942541.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHKV2_004815"
     CDS             complement(399..1022)
                     /locus_tag="ACHKV2_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942542.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHKV2_004816"
     CDS             complement(1025..1306)
                     /locus_tag="ACHKV2_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6942543.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHKV2_004817"
     CDS             complement(1299..1625)
                     /locus_tag="ACHKV2_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO6942544.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3737)
                     /locus_tag="ACHKV2_004818"
     CDS             complement(1713..3737)
                     /locus_tag="ACHKV2_004818"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6942545.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(3682..5184)
                     /locus_tag="ACHKV2_004819"
     CDS             complement(3682..5184)
                     /locus_tag="ACHKV2_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6942546.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(5184..5396)
                     /locus_tag="ACHKV2_004820"
     CDS             complement(5184..5396)
                     /locus_tag="ACHKV2_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942547.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..>6505)
                     /locus_tag="ACHKV2_004821"
     CDS             complement(5393..>6505)
                     /locus_tag="ACHKV2_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO6942548.1"
                     /translation="PAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYL
                     PRSATEQRKSELLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGE
                     RWIVDRYNIRHSLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLM
                     AMAVDTGGEAGVTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSA
                     RRARASGDVALWLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGS
                     DGKWRKPGRGANEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGET
                     VSPELTAGAEKRRRRKKKLRTERAEDNPWITSGGWL"
BASE COUNT         1380 a         2067 c         1547 g         1511 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagacgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgctcagc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccagt gcattcagtt cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ccgcgtcacc cggcccccca
     3661 gcttgcatcc tgaaccagga tttattcttc atgctggctt tcggtgtcgc gctgatactg
     3721 tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg gggtcagtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccaccg gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ctctttgatc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tgatgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggccgctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccccgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacccggggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaagatga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccgcagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ccagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctgg
//
LOCUS       DAZFTN010000197         6441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFTN010000197 DAZFTN010000000
VERSION     DAZFTN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..343
                     /gene="ydjO"
                     /locus_tag="ACHKV2_004822"
                     /pseudo
     CDS             1..343
                     /gene="ydjO"
                     /locus_tag="ACHKV2_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(347..1738)
                     /gene="tcyP"
                     /locus_tag="ACHKV2_004823"
     CDS             complement(347..1738)
                     /gene="tcyP"
                     /locus_tag="ACHKV2_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO6942549.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1871..2461)
                     /gene="ydjM"
                     /locus_tag="ACHKV2_004824"
     CDS             complement(1871..2461)
                     /gene="ydjM"
                     /locus_tag="ACHKV2_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6942550.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2624..3292)
                     /gene="hxpB"
                     /locus_tag="ACHKV2_004825"
     CDS             complement(2624..3292)
                     /gene="hxpB"
                     /locus_tag="ACHKV2_004825"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO6942551.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3439..3975
                     /gene="yniB"
                     /locus_tag="ACHKV2_004826"
     CDS             3439..3975
                     /gene="yniB"
                     /locus_tag="ACHKV2_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO6942552.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4016..4876)
                     /gene="yniA"
                     /locus_tag="ACHKV2_004827"
     CDS             complement(4016..4876)
                     /gene="yniA"
                     /locus_tag="ACHKV2_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO6942553.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4982..5272)
                     /gene="ghoS"
                     /locus_tag="ACHKV2_004828"
     CDS             complement(4982..5272)
                     /gene="ghoS"
                     /locus_tag="ACHKV2_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6942554.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5373..6302)
                     /gene="pfkB"
                     /locus_tag="ACHKV2_004829"
     CDS             complement(5373..6302)
                     /gene="pfkB"
                     /locus_tag="ACHKV2_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO6942555.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1672 a         1595 c         1571 g         1603 t
ORIGIN      
        1 tttttgctgc gattcaaaaa aatgagagtc tcctaaagtt tccttcgata atatcaatgc
       61 ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt ggtatacttg
      121 aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat gaggttttaa
      181 gcttctcgct acaggcacat tcattgagcc aggaatgtat tgataagttg gacatttttt
      241 cgctgacgat ctgtttaaat atgagtcagt atgcatcgcc gcgatggaac atttaaaaag
      301 taaggcaacg gcccctttac aaaacggacc gttgccagca taagaattaa tggtgtgcca
      361 gttcggcgtc gtcttcgcta tccagaatgg ctttatcggt ttgcttcagc cactggctgg
      421 tcagcgtgcc agctgtcatc gagccactaa cgtttagcgc cgtacggccc atgtcgataa
      481 gcggttcaac ggagattaac agcgccacca gggttactgg caggcccatc gcaggcagca
      541 caatcagtgc ggcgaaagtt gcaccaccac cgacaccggc aacgcctgcg gagctaacgg
      601 taacaatacc gaccagcgtc gcaatccaca tcgggtccag cgggttaatg ccaaccgtag
      661 gcgcaaccat caccgccagc attgccggat acaaaccggc gcagccgttc tgaccaatgg
      721 tcgcaccgaa agaggcggcg aaactggcga tggattcagg aacaccgagt cgacgcgttt
      781 gtgcttccac attcagtggg atagacgcag cactggaacg gctggtaaag gcaaacgtca
      841 gcacaggcca taccttacgg aagtacttca gcggactcac gccattaatg cccagcagaa
      901 tgccatgcac tgcaaacata atcagcagac cgaggtagga cgcgacaacg aaacttccca
      961 gtttgatgat gtcttgcagg ttagaacctg caaccacttt ggtcattagt gccagaacgc
     1021 cgtaaggggt caactgcatg accaggcgaa ctagtttcat cacccagctt tgcagggtat
     1081 cgatagcggt taagacgcgt tcacctttcg gcgcatcatc cttcagcagt ttcagcgcag
     1141 ctacaccgag gaatgcggca aaaattacca cgctgataat tgacgtcgga ttggctccgg
     1201 tcagatcggc aaacgggttt ttcgggataa aggacaagac cagctgcgga acgctcaggt
     1261 cagagacttt accaacatag ttactttcga tggcgttcag acgtgcagtt tctgcaccac
     1321 cctgaaccag accttcagcc gtcaaaccaa acaggttggt taccagcaca ccgaccagcg
     1381 ccgcaatcag cgtggtaaac aaaagcgtac caatggtcag aaaactgatt ttgcctaact
     1441 gagatgcgtt atgcagacgg gcaaccgcgc tcagaataga ggcgaagact aacggcataa
     1501 cgatcatttg cagcagttga acatagccgt taccaacaat gttaaaccac tgtacagaat
     1561 ctttaagtac ctggctgtca gaaccataaa tggtatgcag ggcaaggcca aaaaccacac
     1621 ccatcaccag acccaccagc acttttttcg ccagactcca ctgtttatgg cgggtctgag
     1681 ccagcgcaaa cagcagtaca acgaacacca cgatgttcgc aattaatgga aagttcatcc
     1741 ccgttctcct gatcttatta tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat
     1801 taacagaact gtgagctggc tcttatattc aaatggaatt gcttattaca gaaaggcgat
     1861 ttttgccttt ttagtattca acctgatgct taataagccg atgaaactgg atctgcaagg
     1921 tattgatcat ttgcgatgac caacgaacag cgctgttttc gggtaatgaa tgaggcatcc
     1981 ataccgacca gacaaataat gccatgcaga taaaacgttc cagttggtta cccttttggg
     2041 gaaccaggat aggcaagcgg aaacgccagc ggcatggcca gagcagggga acaccggcgg
     2101 gtgtcagcat atcggcaagt atgtggctta aataacccag caccattcct tgtagcgcat
     2161 cagccggaat gaaccagctt tcaggaacct taaggtaaaa ggttgccagc agcgcaaata
     2221 ccgccagcag actgtgggta aaaccacgat gcccaaaagc gcgggcgatc ggttttgata
     2281 tccactttaa tcgctgccca agaaacgact ttgggtgatc gatgtccggt aacaaacacg
     2341 tcaggattgc ggaagggaca atatgccacc agtcaccctg tgccagcacg ggcgtcagct
     2401 cggcattttt ggcaaatacc gcacaagcaa tagaaaagag aaggtgacct tccgccgtca
     2461 tgataaaact cactaaactg tcgatacgta cagtatagga tttttataca gtgcacggaa
     2521 gtggtgacgg tgtaactctt tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat
     2581 tcagataaag aaccaggggc agcgtgatca ctgcccctgg tcattaaccg agaaggtctt
     2641 ttgcggtgag ttctgtcagc gatgagagtt tgacgtttgc taatacaaaa cgtggatcat
     2701 tttgcgcttc tggcgcagga acgacgatgg aacgcatgcg ggctgcttta gaggcgatca
     2761 tgccatttac cgaatcctcc agcgctacgc aggtcagtgg gtcaacgccc agttttgctg
     2821 cgcagtcgag atatacttgc ggatgcggct tgctgtaagg cagtttttcg gccgaggcga
     2881 gggcatcgaa actgtcgcgt aagtcaaaca tggtcaacac tttttccagc atatgtagtg
     2941 gtgacgcgga ggccagtccc accaataaac cttgttcttt gcataacgca acggcttcgc
     3001 gcacgcctgg taataatgga cgtgtctctt caaccagtga aatggcacgg gcaataaccc
     3061 gttctactac ttcctgacgg cttggcccat tccacggttg ccgggcgtac caaagatcga
     3121 ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc gttacgacgg gagatatcca
     3181 cccccaggct tgccatcaca tccagttcgg ctcgatccca taaaggttct gagtcgataa
     3241 gtaatccatc catatcaaaa attgcagcaa gaatctgacg cggggttgac attacaacct
     3301 ctcctttaga gcattatgtg taaggtatta agccgtgctt atgattaagc gcctatcata
     3361 tcgcctttaa agaatgggcg aaaacggata tctacaggta aactaagtga aacgacgcgt
     3421 cttaatcaag gagaactcat gacgtatcaa caagctggac gcattgctgt tttgaaaagg
     3481 attatgggtt gggtgatttt tatacctgca ttgatctcta cattgatttc tttgctgaaa
     3541 tttatgaata cccggcagga aaaccaggaa ggcattaatg cggtcatgct cgactttact
     3601 catgtcatga tcgatatgat gcaggcgaat acgccttttt tgaatctgtt ctggtataac
     3661 tccccgacgc ccaattttaa tggtggcgtg aacgttatgt tctgggtgat tttcatcctg
     3721 atttttgttg gactggcgtt gcaggattcc ggtacccgga tgagtcgcca ggcgcgcttc
     3781 ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag ccaaagggga agaggggcta
     3841 acgcgtgaac aaattgagtc acgtattgtt gttccgcacc atactatttt cctgcagttt
     3901 ttctcactgt atatcctgcc ggttatttgt attgctgcag gttatgtgtt cttttctctg
     3961 cttgggttta tttaatcaaa aggccgcacg gagcggccat tatcttcaac ccatatcacg
     4021 cagctaataa tctatccagt gactgctgag caataaccaa atgctgaccg ccaaataatc
     4081 ttgcacgatt taacagcgtg tagagttggt aaaccggttg acgttcaagg aaatcggcag
     4141 gtagcgggga tactgactga tagccgtcat agatttgtgg cggctgttca gtatgcagcg
     4201 gtaacatcgc caggtcgcac tctcggtcac cccagtagca ggccgggtcg aaaatgtacg
     4261 ggccatccgg acccagcgca cagttgccgg accataaatc gccgtgcaac agagaaggct
     4321 gcggttgatg tgaggcgaga cgctgctgaa tatgctcgac gatggcatcg atattgccga
     4381 aagcgatccc tttttctgct gccagttcca actgccagcc aatccgttgt tcagcaaaaa
     4441 acgttgacca gcgacgttgc caggtgttgg gctgtggagt tgtggagagc gagttatcaa
     4501 aatcgagtcc aaattgtggt tggtcgctcc attgatgtaa acgcgcaatt tgctgaccaa
     4561 gaataaatgc gctatgcgca tccagcggac gaggtgggag ataatccatc accagaaaac
     4621 tgtagtcacg gtcagcgcca actgcccaaa ccttaggcac ggtgacggtt ttactacgcg
     4681 acagtaactc cagttggtcg gcttcggcgg taaaaccggg aagcagttcc ctttcatcac
     4741 atttgacgaa aaagtcatgt cctgcatagc gcaaatgcca tgcggcatgg acttctccgc
     4801 caggcagttc attacgcagt tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac
     4861 tgattgcctg ccacatgatg tctctcccat gttgtctgcc agatcataag gttagcgcaa
     4921 gaatgcggtg taaaaacacg agctgacgca caccacagac tgattattgc ccaaaaacca
     4981 tttattttct gttgctattc cattcctccc aggtcgtgat ggtgatttca ggatctttgc
     5041 cggttgcact ttgggtaagc cccgaaacca gttctctaat ttcttcttca ctttgggtac
     5101 tgataagtcc aaaagtgtta gtccccagct catggatatt tccctcatca tccgtcatgg
     5161 tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc gttaatttca gtcaatgtat
     5221 cctcatgcaa catgacggtt atgacataac ggacaagatc gccactggcc atagttcacc
     5281 cctttgttat cacatgactt ttgagcatag tcggagaaac gcgttgccga caggttggtg
     5341 atgattcccc caatgctggg ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt
     5401 tgcgtatcgt catgggagca cagacgtgtt ccctgattga gtgtggctgc actccccgca
     5461 gctacgccaa aacgaaccat ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc
     5521 gcgccgacca tgctatcacc agcgccaacg gtactctggc ttttcaccgg tggtggcacc
     5581 acctgaatgc agttttcact atcaacaccc agcgctcctt gtggacccag ggaaacgaca
     5641 acccgtttgg ccttgccgct attaacgatt tcctgcgcgg ctttgcggac atcgtctggc
     5701 tgggtgagtt cgcgattcac cagcgcactg agttcttttt ggttaggctt aaccaactcg
     5761 atgttaccaa tcgccagtgc tgcacttaac gcttcgccag aactgtcgat gatgcagcgg
     5821 atcccttggt tttgcgcagc ggaaatcagt tgggttaatt tttccagctt cacacctggc
     5881 ggcaggcttc cgcttatgac caggatggcc ccggattcaa tttccagaac ttgctcttca
     5941 agctggcgaa actcatcttc atttaatgcc gcgcctggca taacaaaacg atactgctca
     6001 ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc agtctttggc ttctacagta
     6061 gcgacgggga cattttcatc cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc
     6121 gccgggaaga tcgctgtggc actgcctcca agatgggcaa tggcgcgggc gacgttgatg
     6181 ccgccgcccc cgggttcgaa caccggtgcg gtacagcgca gttttccttc gggataaatt
     6241 tgcggggtaa ttgttgcgct atcgagagag ggcgcaagtg tcaacgtata gatacgtacc
     6301 atcatttcct cctataggct gatttcagtc tggcaccgaa tcagcggaaa gtgaagaaat
     6361 taacaatatg atttattgga gctttattta aaattttgca gataaatata tataaataaa
     6421 aatctgtttt cgccacatgc t
//
LOCUS       DAZFTN010000198         6394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFTN010000198 DAZFTN010000000
VERSION     DAZFTN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..355)
                     /locus_tag="ACHKV2_004830"
     CDS             complement(71..355)
                     /locus_tag="ACHKV2_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO6942556.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            633..3065
                     /gene="hsdR"
                     /locus_tag="ACHKV2_004831"
     CDS             633..3065
                     /gene="hsdR"
                     /locus_tag="ACHKV2_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO6942557.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3132..4601
                     /locus_tag="ACHKV2_004832"
     CDS             3132..4601
                     /locus_tag="ACHKV2_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6942558.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4601..6355
                     /locus_tag="ACHKV2_004833"
     CDS             4601..6355
                     /locus_tag="ACHKV2_004833"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6942559.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFS
                     RQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQS
                     AQQTQLHLADALTDAAVN"
BASE COUNT         1930 a         1487 c         1453 g         1524 t
ORIGIN      
        1 attagtcttg gttcatctgt ttcatcagaa taaacatcaa taggcagaac tcttttttgc
       61 aaatcatctt ttatcccctg aatcgggatg atttttttag ctcggagaca ataatgctta
      121 acgttgtaag acgttgttgt ccatctacaa cttcatactg attttgccct ttattgatac
      181 aaatcatact cccaataaaa tatccattct cttctgattg agagctttta taaatgtcat
      241 caatgagttg ctctgcatta tctaccgtcc aactgtaagg acgctgataa gaaggaataa
      301 taaacttttt gttttcagta aggatatcct gcaccttaca ttctttagct tccatttcat
      361 atacccaatc aaacaatcaa caataacatt atctttcttg ctcgaaagaa acgcaactat
      421 aagattttta tatattttgt tagagttgat atggttcctg taacttctgt ttatctgtct
      481 ctgatatgcc cagattaatt caagcaagat ataacagaga tatcaatgtg atctctgcta
      541 cgtactttac gttgaaacac cgccctaaat catagccagg ctattgattt tatgtacaat
      601 cagaatcgtc ctgataaaag cgagaatacc ccatggcgga actgaaccta agtaacctga
      661 cggaagcaga catcattacc aaatgcgtta tgccagccat tctcaatgca ggctgggaca
      721 acacaacgca aatcagacag gaggtcaaac tccgggacgg taaagtcatc gtacgtggta
      781 aagttgcggc acgcagaacg gtaaaatctg ctgacatcgt gctgtatcac aaacctggca
      841 ttcccttagc cgtgattgaa gcaaaagcca acaaacatga aattggcaaa gggatgcaac
      901 agggcattga atatgcgcgc ctgctggacg ttccttttgt ttttgccacc aacggtgatg
      961 gctttatctt ccgcgatgcc accgcagccg aaggtgaatg ccttgaaaag caaataacgc
     1021 tggatgactt cccctccccc gctgaactct ggcaaaaatt ctgcctctgg aaaggataca
     1081 cacaagctca gcttccggtg attactcagg actattacga cgatggcagc ggtaaatcgc
     1141 cacgttatta ccagcttcag gcaatcaaca aaaccattga agccgtctcc aacgggcaaa
     1201 accgcgttct gctggtcatg gcgaccggaa cagggaaaac ctataccgca ttccagatca
     1261 tctggcgcct gtggaaatca aaaaataaaa aacgcatttt gttccttgcc gatcgcaata
     1321 ttctggtcga ccaaaccaaa aataatgatt tccagccatt tggtacggca atgaccaaag
     1381 tcagcggacg caccattgat cccgcttatg aaattcacct cgcgctctat caggctataa
     1441 ctggcccgga ggaagaccaa aaagcgttta aacaagtcgc accagatttc ttcgatctga
     1501 tcgtgatcga cgaatgccat cgcggcagcg catccgaaga cagcgcctgg cgagaaatcc
     1561 ttgattattt tagttccgcc acccaaattg gcttaaccgc cacgccaaaa gagacgcatg
     1621 aagtctccag cacggattac ttcggcgatc cggtttacgt ctactcacta aaagaaggga
     1681 tcgaagacgg cttcctcgcc ccttataaag ttgtccgtgt tgatattgat gttgatctgc
     1741 aaggctggcg cccaaccaaa gggcaaactg acttaaacgg cgaagtgatc gacgatcgta
     1801 tctataacca gaaagatttc gatcgcacga tggtaatcga cgaacgcaca gaactggttg
     1861 ccagaaccat taccgactac ctcaagcgta ccaatccgat ggataaaacc atcgtcttct
     1921 gtaacgacat cgatcatgca gaacgtatgc gccgcgccct ggttaatctc aacccggagc
     1981 aggtgaaaaa gaacgacaaa tacgtcatga aaatcaccgg cgatgatgaa attggcaaag
     2041 ctcagttgga taacttcatc aacccgaaaa aaccgtaccc ggttatcgcg accacttcag
     2101 agctgatgac caccggtgtg gatgctaaaa cctgcaaact ggtagtactg gaccagaaca
     2161 tccagtcgat gaccaaattc aagcagatta tcggtcgtgg tacacgcatc gacgaacgtt
     2221 acggcaaact ctggtttacc atcctcgact ttaaaaaagc caccgaactg tttgccgatg
     2281 agcgtttcga tggcattccc gaaaaagtca tggataccac accagaggat atcgccgatc
     2341 cagaatctga ttttgaagag aaactcgaag aaatcagcga acatgacgac gaacaggtaa
     2401 caggcgttga tgaaccgcct gcgccaccat accaggttaa agataccgat gatgtcggcc
     2461 cacttccgga agaagacgag aagaaaatcc gcaagtttca cgtcaacggt gtagcagtgg
     2521 gcgttattgc ccagcgtgtt cagtattacg acgccgacgg taaactggtt accgaatcct
     2581 ttaaagatta cacccgcaaa acactgctca aagaatatgc ctcgctggat gactttaccc
     2641 gcaagtggca ggacgccgat cgcaaagaag cgatcattca cgagctggag caacagggga
     2701 tcatctggga agtactggca gaagaagtcg gtaaagatct cgacccgttc gacatgcttt
     2761 gccacgtagt gtatggtcag ccgccgttaa cccgcaaaga gcgcgccgag aacgtgcgca
     2821 agcggaacta cttcacaaaa tactctgaag cagcgcaagc cgtgctcgat aatctgctgg
     2881 ataaatacgc cgatgcgggc gtacaggaga tcgaaagtat tcaggtgctg aaacttaagc
     2941 cattcgacag catgggcacc ttaccggaga ttattaaaac cggatttggc gaccgtaacg
     3001 ggtataatca ggcgctcagc gagctggaaa acgaaatcta ccaattaccg ccccgctctg
     3061 cttaacaact ctcttttgcg ccttctgcaa ccatcatgtt gcagaaggcc ttgtttttca
     3121 tggaatacaa catgtctatc agctcagtaa tcaaatcatt acaagatatt atgcgcaaag
     3181 atgccggtgt ggatggcgat gcgcagcgtc tcggtcagct ctcctggctg ctgtttttga
     3241 aaatcttcga tgcccaggaa gaggcgctgg aactggagca ggataactat caatatccga
     3301 tcccacagcg ttatttatgg cgcagttggg ccgccaacgc gcagggcatt accggtgatt
     3361 ccctgctgga attcgttaat gatgatctgt tcccggcgtt gaaaaacctc actgcgccta
     3421 tcgataaaaa cccacgcggc tacgtggtga agcaggcgtt cagcgatgcc tataactata
     3481 tgaaaaacgg tacgctactg cgtcaggtga tcaataagct gaacgaaatt gactttacca
     3541 gcgccagcga acgccatctg tttggtgata tttacgaaca gatccttaaa gatctgcaat
     3601 ctgcgggcaa tgcgggcgaa ttctatactc cacgcgccgt cactcgcttt atggtggatc
     3661 gcgttgatcc gaaactcggc gaatccatta tggacccggc ctgcggtacg ggcggttttc
     3721 ttgcctgcgc atttgatcat gtaaagaaca aatacgtgaa gagcgtcgcc gatcatcaga
     3781 cgctgcaaca acagatccac ggtgttgaga aaaaacagct tccgcacctg ctggcgacca
     3841 ccaatatgct gctacacggc attgaagtgc cagtgcaaat tcgtcacgac aacactctga
     3901 acaaaccgct ttcctcccgg gatgagcaac tggatgtcat tgttaccaac ccgccgtttg
     3961 gtggcacgga agaagacggt attgagaaga actttccggc agagatgcaa acccgcgaaa
     4021 cggcggattt gttcctgcaa ctgattgtgg aagtactggc gaaaaatggt cgcgcggcgg
     4081 tggtattgcc ggatggcaca ctatttggcg aaggcgttaa aaccaaaatc aaaaagctac
     4141 ttaccgaaga gtgcaacctg cataccatcg tgcgcttacc gaatggtgtg tttaacccct
     4201 ataccggcat taaaaccaac ctgttgttct ttaccaaagg tcagccaacc aaagagattt
     4261 ggttctatga gcatccgtat ccggcgggcg taaaaaacta cagcaaaact aagccgatga
     4321 agtttgaaga gtttcaggcg gagatcgact ggtggggtaa cgaggccgat ggttttgcca
     4381 gccgcgtaga gaatgagcag gcgtggaaag tcagcattga tgacgtgatt gcgcgtaact
     4441 tcaatctgga tattaaaaac ccacatcagg cggaaaccgt cagccatgat ccggccgagc
     4501 tgttagcgca gtatgcaaaa cagcaggcgg agatccagac gctgcgtaat caactgcgcg
     4561 atattcttgg cgctgcgctg tctggcaagg aggttaacta atgagtgtgg aaaagctgat
     4621 cgttgatcat atggaaacct ggacctcggc gttgcaaacc cgttccaccg ccgggcgcgg
     4681 cagttccgga aaaattgatt tatatggcat taagaaatta cgtgagctga ttctggaact
     4741 ggctgtgcgc ggtaaactgg tgccgcagga cccgaacgat gaaccggcgt cggagctgct
     4801 gaagcgtatt gcggcagaaa aagcagagct ggtgaagcaa ggtaaaatta aaaagcaaaa
     4861 accactgccg gaaattagtg aggaagagaa gccgtttgaa ttgccggagg gatgggagtg
     4921 ggtgcgtatt agtgaaattg gacatgactg gggacaaaaa actccagata aagattttac
     4981 ttacattgat gttggttcaa taaataaaga atatggaatt attgaagagc tatctattct
     5041 ttcagcgaaa gatgccccat cgcgagcgag aaaaattgtt cagcaaggga ctatcatata
     5101 ctctactgtt cgcccgtatt tgttaaatat agctattatt gaaaacgaaa tactacctga
     5161 accgatcgct agtactgcct tcgctattat tcatccatat acggcgatgg atgctaattt
     5221 catttattat tatctacgtt ctccggtatt tgtttgttat gttgaaaatt gtcagacagg
     5281 ggttgcctat ccagcaatca atgacaaaca atttttttct ggaataactc cagttcctcc
     5341 atccttggaa caagttcgca tcgcaaacaa aatcaaagaa ttaatgtccc tctgcgacca
     5401 actggaacag caatccctga ccagtctgga cgcacatcaa caactggttg aaaccctgtt
     5461 gggaacgctt acagacagcc aaaccgccga ggaactggct gaaaactggg cgcgtattag
     5521 cgagtatttc gacactctat ttaccaccga agccagcgtg gatgcgttaa aacagaccat
     5581 tctgcaactg gccgtaatgg gcaaacttgt gccgcaggat ccgaatgatg aaccagcctc
     5641 tgaactgctc aaacgaattg cgcaggaaaa agctcaactg gtgaaagaag gaaaaataaa
     5701 aaaacaaaaa ccgttgccgc caattagcga tgaggaaaaa ccgtttgaac ttccggaagg
     5761 gtgggagtgg tgtttatttg aagatattat tgatattcaa agtggtatca ctaaaggaag
     5821 aaatttatca aatagaactt tggtaaaagt tccttattta cgtgttgcaa acgtccaacg
     5881 cggatatctt gatcttacgg aaattaaaca gattgaaatc cctattgagg aaaaagaaaa
     5941 atatcaagta gtcaagggag atttattgat aacagaaggc ggcgactggg atacagtcgg
     6001 gagaactaca gtatggtgtc atgactggta tatagcaaat caaaaccatg tattcaaagg
     6061 acgaaatata gggcaagatg ttgatccata ttggttagaa acatatatga atagcccatt
     6121 ctcaagacaa tattttgcta acgcaagtaa gcaaaccact aatttagctt ctattaataa
     6181 aacccagctc agaggttgtc ctgttgctat tcctcctagc tcagaagcaa aaaaaataat
     6241 gagtaaacta catatttttt ataaactatg tgaagaatta aagaatcata tccaatccgc
     6301 ccagcaaacc caactacacc ttgcagatgc actcactgac gcggcggtaa actaatttac
     6361 ctgccctcga aaaaccaata aagcggctaa atag
//
LOCUS       DAZFTN010000199         6350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFTN010000199 DAZFTN010000000
VERSION     DAZFTN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..131)
                     /locus_tag="ACHKV2_004834"
     CDS             complement(<1..131)
                     /locus_tag="ACHKV2_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942560.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTED"
     gene            460..1332
                     /locus_tag="ACHKV2_004835"
     CDS             460..1332
                     /locus_tag="ACHKV2_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6942561.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1704..4553
                     /gene="cah"
                     /locus_tag="ACHKV2_004836"
     CDS             1704..4553
                     /gene="cah"
                     /locus_tag="ACHKV2_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO6942562.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4664..>6350
                     /locus_tag="ACHKV2_004837"
     CDS             4664..>6350
                     /locus_tag="ACHKV2_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO6942563.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1599 a         1604 c         1785 g         1362 t
ORIGIN      
        1 ttatcctcag tattatcata gccgaaaact tctcccggac acgggcgttc acgataatag
       61 tcatcgtcat agttttcatc gtcagataat gatatgtcgt attctgaata tgattcacca
      121 caattataca tgaaatcatc tcctttgggg ggggcaataa ttagtgagtt tattcgaaaa
      181 aatgatgagc gccagaccaa tccacccaac ttttgattat aatcatgaag atacttgtct
      241 cagtacaaat tgaatcaaac tcaatgcagc atgattcacc tttatattct ctcagggaaa
      301 ccaaatacca ctactcaact attaagaaca aaattcggga tgtatgatta tcagaagata
      361 cctgcatcct aaataatttc agacagatat tatcgttgtg aatcccccgc cacctttccg
      421 gtgcgcgggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc tgatgccatt
      481 gaggcaattg aaaaaccgct ctcctccctg ccttactcgc tttcccgtca catcctggaa
      541 catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa aagcggggcc
      601 ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt cagtgatgtt
      661 cacgccggca cccgggaagt gcggcgcttc cgtctgagtg gccatggtca cagcatggtt
      721 atcactgacc tgcccggggt gggcgagagc cgggacaggg atgcagagta tgaagccctg
      781 taccgtgaca ttctgcctga actggacttg gtactgtggc tgattaaagc cgatgaccgt
      841 gccctgtctg tggatgagta tttctggcga cacatactgc accggggaca tcagcaggtg
      901 ctgtttgtgg tgacgcaggc cgacaaaaca gagccctgcc atgaatggga tatggcaggt
      961 attcagcctt ctcccgcaca ggaacagaac attcgcgaaa aaacagatgc ggtattccgg
     1021 ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg ggaactggat
     1081 acgctggtca gtgcgctcat gacagcgctt cccgaccatg ccgccagtcc cctgatgacc
     1141 cgcctgcagg acgagctgtg cacggaatct gtctgggggc aggcccgtga acagtttacc
     1201 ggtgcggtgg accggatatt tgacacggtg gagagcgtct gtgttgcctc tgttgcacgc
     1261 acagttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgcggt atggaactgg
     1321 attttcttct gaacagtctg ccaggggaaa tgtctgatta cattgcgtta accaactgaa
     1381 aacatgatac aaacacctca tgcccctcct gtttctcttg cgtgactgct ctactgttaa
     1441 tagaataaaa cgatcgataa aacaggtatc gatcgtttat atcgatcgtt tatagtggtt
     1501 aatggcctgc gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaaca
     1561 gactgatact tctctgtctg atgaaggggg agagcgttta cgcccgggac gtatcgctgt
     1621 gcccgataac tctgttttgt acctgccggt atccactttt gtgggtaccg gcttttttat
     1681 tcaccctctg taaggaaaag ctgatgaaac gacatctgaa caccagctac aggctggtat
     1741 ggaatcacat tacgggcacc ctggtggtgg cctccgaact ggcccgctca cggggaaaac
     1801 gcgccggtgt ggcggttgcg ctgtctcttg ctgctgtcac atcagtcccg gcactggctg
     1861 ctgacaaggt tgtacaggcg ggagaaaccg tgaacgatgg aacactgaca aatcatgaca
     1921 accagattgt cttcggtacg gccaacggaa tgaccatcag taccgggctg gaactggggc
     1981 cggacagtga agaaaacacc ggtgggcaat ggatacagaa tggcgggata gccggaaaca
     2041 ccactgtcac cacaaatggt cgtcaggtcg tgctggaggg gggaacagcc agtgatacgg
     2101 ttattcgtga cggcggggga cagagcctga acggactggc ggtgaacacc acactgaata
     2161 acagaggcga gcagtgggtg catgagggcg gggttgccac cggtacaatt atcaaccgcg
     2221 acggttacca gagcgttaaa agtggcgggc tggcaacagg aaccatcatc aacaccggcg
     2281 cagaaggcgg ccctgattct gacaactcgt atacgggtca gaaggtccag ggaacagcag
     2341 aatccaccac catcaacaaa aatggacggc agattatctt attttccggg ctagcccgtg
     2401 acactctcat ttacgcaggt ggtgaccagt cggtacacgg aagggccctg aataccacac
     2461 tgaatggcgg ttaccaatat gtgcacaggg acggacttgc gctgaacacg gtaattaacg
     2521 aggggggctg gcaggttgtt aaggcaggtg gcgctgccgg taacaccacc ataaatcaga
     2581 acggtgaact gagggtacat gccggcgggg aagccactgc agtcacccag aacacgggcg
     2641 gtgcactggt taccagtact gctgcaactg tcatcggcac aaaccgtctg gggaatttca
     2701 cggtggaaaa cggtaaggct gacggtgttg ttctggaatc cggcggtcgt ctggatgtac
     2761 tggagagcca ttcagcacag aataccctag tggatgacgg cggtaccctg gcagtgtctg
     2821 ccggcggtaa ggcgacaagt gtcaccataa catccggtgg tgccctgatt gcagacagtg
     2881 gtgccactgt tgaggggacc aatgccagcg gtaagttcag tattgatggc acatccggtc
     2941 aggccagcgg cctgctgctg gaaaatggcg gcagctttac ggttaatgcc gggggacagg
     3001 ctggcaacac cactgtcgga catcgtggaa cactgacgct ggctgccggg ggaagtctga
     3061 gtggcagaac acagctcagt aaaggcgcca gtatggtact gaatggtgat gtggtcagta
     3121 ccggcgatat tgttaacgca ggggagattc gctttgataa tcagacgaca ccgaatgccg
     3181 cgctgagccg tgctgttgca aaaagtaact ccccggtaac gttccataaa ctgaccacca
     3241 cgaacctcac cggccagggc ggcaccatca atatgcgtgt tcgccttgat ggcagcaatg
     3301 cctctgacca gctggtgatt aatggtggtc aggcaaccgg caaaacctgg cttgcgttta
     3361 caaatgtcgg aaacagcaac ctcggggtgg caaccaccgg acagggtatc cgggttgtgg
     3421 atgcacagaa tggcgccacc acagaagaag gtgcgtttgc cctgagtcgc ccgcttcagg
     3481 ccggcgcctt taactacacc ctgaaccgtg acagcgatga agactggtac ctgcgcagtg
     3541 aaaatgctta tcgtgctgaa gtccccctgt atacatccat gttgacacag gcaatggact
     3601 atgaccggat tctggcaggc tcccgcagcc atcagaccgg tgtaaacggt gaaaataaca
     3661 gcgtccgtct cagcattcag ggcggtcatc tcggtcacga taacaacggc ggtattgccc
     3721 gtggagccac gccggaaagc agcggcagct atggcttcgt ccgtctggag ggtgacctgc
     3781 tcagaacaga ggttgccggt atgtctctga cgacaggggt gtatggtgct gcaggccatt
     3841 cttccgttga tgttaaggat gatgacggtt cccgcgccgg cacggtccgg gatgatgccg
     3901 gcagtctggg cggatacctg aatctggtac acacatcctc cggcctgtgg gctgacattg
     3961 tggcccaggg aacccgtcac agcatgaaag cgtcatcgga caataacgac ttccgcgccc
     4021 ggggctgggg ctggctgggc tcactggaaa ccggtctgcc cttcagtatc actgacaatc
     4081 tgatgctgga gccacaactg cagtacacct ggcagggact ctccctggat gacggccagg
     4141 ataacgccgg ttatgtgaag ttcgggcatg gcagtgcaca acatgtgcgt gccggtttcc
     4201 gtctgggcag ccacaacgat atgacctttg gtgaaggcac ctcatcccgt gacaccctgc
     4261 gcgacagtgc aaaacacagt gtgagtgaac tgccggtgaa ctggtgggta cagccttctg
     4321 ttatccgcac cttcagctcc cggggtgaca tgagcatggg gacagccgca gccggcagta
     4381 acatgacgtt ctcaccgtcc cggaatggca cgtcactgga cctgcaggcc ggactggaag
     4441 cccgtatccg ggaaaatatc accctgggcg ttcaggccgg ttatgcccac agcgtcagcg
     4501 gcagcagcgc tgaaggctat aacggtcagg ctacgctgaa tatgactttc tgattatacg
     4561 gtatcccgga aatacgattt caggaaaatt catgcagtaa tgggaaagga cgctcattgc
     4621 tgttcaccgc agttctgaat tattgaattt ataaaggtga atgatgcacg aaaagaacat
     4681 cgccctgctt tgtgatgaag ccgaccggct tttgcaactg aatatcaatc tgctccggca
     4741 aatggttgat gagccggatg tgttatctga cagtaagaac gaaaacgggc tgctttttga
     4801 taaacggaaa gcgctgaaaa gaattgagga gctggagggc gaacaaatca aaaccgcccg
     4861 cagggagatg gtgctggctg tggtcggcac gatgaaggcg ggcaaatcaa ccaccatcaa
     4921 cgccattgtg gggcaggaaa ttctgcccaa ccgtaaccgc cccatgacct cggtaccgac
     4981 attgatccgc cacgttcccg gaaaaacgga accggtcctc catctggaac atattcagcc
     5041 cgtccgcaat ttattaatca cactgcagga aaaactcgcc accccggcag ggcagcaagt
     5101 cgcacagtcc ctgcagcaaa ccggggatac ccgcgaacta ctggatattc tggcggatga
     5161 tgtctggctt aaaaatgaat accacgggga agacgaaatc tttaccggac tggcatcgtt
     5221 aaatgacctg gttcgtcttg ctgcggcaat ggggactgaa tttccttttg acgaatacgc
     5281 agaagtgcag aaactaccgg tgatcgacgt ggcgttcagc cacctggtgg gaatggatgc
     5341 atgtcaggga acactcacac tgctggatac ccccggccct aatgaagccg gacaaccaca
     5401 gatggaaatg atgatgcggg atcaactaca gaaagcctct gcggtgctgg cggtgatgga
     5461 ttacacccag atgaactcga aagccgatga ggaggttcgt aaagagctta atgccattgc
     5521 tgacgtctca gccggtcgtc tgtttgtgct ggtcaataaa ttcgatgaaa aagatcgcaa
     5581 tggtgatggt gcggatgccg tacgccagaa ggtgccagca atgctgaaca gcgatgtgct
     5641 gcccgcctcc cgcgtttatc ccggctcatc acgtcaggcg tacctggcta accgtgcgct
     5701 tcatgagtta cgaaaaaacg gtgcgcttcc ggttgatgaa gcctgggtcg atgattttat
     5761 cagggaagct ttcggaccta tggtggaaga ggacgactgg aaagacagca ctaaggtaaa
     5821 taaaaaggca gaaaaactct ggaatatatc tctgatcgat caactgataa cggaagtcat
     5881 acagagctcc cattccagag cggcggcact ggcggttgac tctgccgccg caaaactgat
     5941 gcagaatgca gaaaatgtca gcgaatatct gtcattacgt catcaggggc tgcagcaaag
     6001 cattcagtca ctacaggcgc atattaccag cctgctggcg gatatccagg agattgagga
     6061 atgtcagaat caggtgaccg gtgatgtcag aatggccatg gaggacatca ataccaaaac
     6121 cggggaatta ctgacgaagg tctgtgcctc actggaggag gagctgaacg actatttcag
     6181 aagcggtaaa cgcaaagaac agcaaatgct ggaggaagaa aattcagcgc agccccggga
     6241 gagaaacgca ttcgctttct ttcacgatat tttcggaacc ggaaatcagc atgacaggat
     6301 gcgggacttt gatccggaca gccccgaaat aaaattcagt gatcgcaggg
//
LOCUS       DAZFTN010000200         6343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFTN010000200 DAZFTN010000000
VERSION     DAZFTN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..186)
                     /locus_tag="ACHKV2_004838"
     CDS             complement(<1..186)
                     /locus_tag="ACHKV2_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6942564.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFF"
     gene            complement(183..617)
                     /gene="psiB"
                     /locus_tag="ACHKV2_004839"
     CDS             complement(183..617)
                     /gene="psiB"
                     /locus_tag="ACHKV2_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6942565.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(672..2630)
                     /locus_tag="ACHKV2_004840"
     CDS             complement(672..2630)
                     /locus_tag="ACHKV2_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6942566.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            complement(2689..2922)
                     /locus_tag="ACHKV2_004841"
     CDS             complement(2689..2922)
                     /locus_tag="ACHKV2_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO6942567.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(2980..3507)
                     /locus_tag="ACHKV2_004842"
     CDS             complement(2980..3507)
                     /locus_tag="ACHKV2_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6942568.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLCKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(4255..4755)
                     /locus_tag="ACHKV2_004843"
     CDS             complement(4255..4755)
                     /locus_tag="ACHKV2_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO6942569.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRLARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(5217..5813)
                     /locus_tag="ACHKV2_004844"
     CDS             complement(5217..5813)
                     /locus_tag="ACHKV2_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942570.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(5810..>6343)
                     /locus_tag="ACHKV2_004845"
     CDS             complement(5810..>6343)
                     /locus_tag="ACHKV2_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6942571.1"
                     /translation="APALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAGHRLF
                     PSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPATVGS
                     WLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRGAPVQ
                     VRALEQWMIPNKLENVI"
BASE COUNT         1321 a         1987 c         1726 g         1309 t
ORIGIN      
        1 aaaaaagcgg agatcggtca gcgaaatccg gcgactgcca ttcaggcagc gaaagaaagc
       61 ggtggcataa ggccatgctg aaagtgtgcg tccctgacga cgacgttgtt ctgtgacagc
      121 caccgcctgc atggcggcct gttgttctgc gctgaggggg atcagtgccc gtgaacgtgc
      181 gctcatgcct gacctccttc tgccatcagg atatggatga tgtcgttaac actgtatccc
      241 tgcgcatcca gtgtcgcggc aagcgccagc gtgtgactga tcgcggcagg cgtgaagata
      301 tgttgcacct gaacgacggc gaaaggctgt ccaccaccat tcaccagaac cagcatccag
      361 agctgagaga cgtctcccgg cgaacataat gccaggtgga aacgttcgtg ggctggcgtg
      421 aaacgaacct ggacggggaa aaagcccccg aactcgctgc catactctcc gttcatcatc
      481 cagttagccg gtgcgtccac tttacgcatc acctcgtgag tgagattacg gcgcatatcg
      541 cttcccgcag cacggatttc ttcatattcc tgggcattca tggactgcag ggcattcagt
      601 gtcagttcgg tattcatcat cgtatttctc cctctgccgc cccgtctccg gcggggcggt
      661 gtggttgttc atcaggcggc atcagccagg ttatcggcgc gttcagtggc atccgtctgt
      721 ggacgtggtg aacacatcca gccaggcacc cagcggttgt ctttcatatg gaactctgca
      781 tgttcagcgg catcgccttt cttcatcttc tctgcgtccc gtgcggcacc ggacagtccg
      841 gcatcattca gggctgcgat aatctgcggc tttttcaggt gtccgaagaa gtttgctttc
      901 gtcggctgcc accagtcgcg catgtgaaaa ccgatggcgc tctccagtga gtcaagcgga
      961 ctgcgtgacg tgcgaccaca ctcgcgggtc tggacaccgt taaggctgca tgcggtgcag
     1021 aaactgagca gggataacag cacctcctga ctgagtgaca ggaacgtcgt catgtcccgt
     1081 gaccatccct ccggtaacag ggccgcaaga cgggcttttt ctgccatcag cgccaggaat
     1141 gcggcacctt ccttccctga cggcgcatcg ctggtcagcg aataatgttt acattccagg
     1201 ctgatttgtg ctggtttact gtacgctccg ctgtcaaaca cattcaggca gagcgtccat
     1261 gccagcagtg ccagagattt gtccggttgc tgcatcagtg ctgcctggac tgccagcgtg
     1321 cgttcggaag acattttcgt cagcaacggc agactgattt cctccactga cgctttctcc
     1381 tgcacctgtt ccgtgcggtc agcgtcatca gtcgcgtcat cttcactgcg caactgcaca
     1441 ccacgctgga cacatacgtt tccataacgc caggacacca ccacgccact tccggcacgc
     1501 atctccggcg tccacgccct gaccttcgcc atgcagcgca tcagcttcat ttctgcttcc
     1561 agcaggtcgg attcctcaca ctggttttcc agcgcgtcgt aacgcgtcat cagttcgttc
     1621 aggcgttctt cttctgcctc cgtcagcacc gcttccggct ccggcagaca gcggtatgtt
     1681 ccggcatcct cacggcactc accgacaggc tccatgcgtc cggcgcacca ttcccagcct
     1741 tcggcttccc gaaggtgttc agcgacagcc tggagttttt ccagcagggc agcatcgagc
     1801 gccacgcggt cgacataacc gtccccgtca tcgctgaaca aatcagtacg cagttcgtca
     1861 ggtgagaaag catcagcccc cacgaagcgg aatttactgt tacctgccac cgccacttca
     1921 ctttcggtca ccagacgacg aatggtctgt acttccggtt taccgcccca tcccgactgg
     1981 caggcggctt caaacacctg cacctgacgc gcggtgtcgt tctccagcgc cagcgcctga
     2041 cagtgctctg tggtgatgcg gtcttctgcc agcgcatcga ggatgacagg cgcaaggtct
     2101 gccagtttca gcattcgctg aacgtggcgg ggcgaatagc ccagcaaatc accgatttgt
     2161 gcaggcgttt tgccttcctg cgccatcgca cggaatccgg caatctgttc ggcagggtgc
     2221 atatcccgac gatgaccgtt ttcagtcatt gatgcagccg tcgccagctc ctgcggaata
     2281 accttcacgc gtacaggcca gtcagccgga atgatgccac gctctgccag catgttgagt
     2341 gcagccagtc ggcgaccacc tgcggcgaca ccgtaacggt caccaggcag ggtatgcacg
     2401 accagattct gcagcagtcc gacgccctta atggactccg ccagttcgct gacggactcc
     2461 gcagaatacg gcaccgtgcg cacattcagc ggtgatttaa tcagcgaggc cagcggcacg
     2521 ctcacttcct ccgtctgcgc cagcagggca gacaggacag tttcctgcgt ttttacagat
     2581 ttacggcttg ctttgcgggt ggtttctggt tcacacttcg ttacaggcat aatcatcccc
     2641 ttataattgc ttatgtcctg ccggacgttc gcgcgtccgg cacctctctt acttaagaat
     2701 cccgaaatcc ctgatgtact ggttcatcca gtacccggca cagtcctcaa aattccagga
     2761 gcgccagatc aacgtgccat cctgacggac aaccaggcga aaatgcgttc cctgatcatc
     2821 ctcgataaag acgttccggt actgtgcggc aacggcttcc gcctgttcgc gggtaaagga
     2881 gccgtccggc agtccctgaa gtattctgaa atattcgctc atcctcatat ctccggtgat
     2941 gccccgcaca gggcggggcg gttgtcacag tcagcccgat cagaacggga tgtcgtctga
     3001 aaacccgtaa tcctccccct ccggcggttg cggctgaggc tctggctgcg cggccttacg
     3061 tccacggcct ttcgttttcg cgccaccttt tttcgtcgcg tcagcactct gtggctgccc
     3121 attctgctga ggcttcggtt gcgcctgagc gttctgctgt ggtgcacgtc ccagcatctg
     3181 cacggtgccc gtggtcttaa caagaatttc agtgacgtaa cgggtgatac cgttatcttc
     3241 ccagctacgg gtgcgaagct gaccttcgat gtagacctgc gcacccttgc acagatattc
     3301 acctgccact tccgcgagct tgccgaacag caccacgcgg tgccattccg tctgctcccg
     3361 catctccccc gtctgtttgt cacgccagct ttctgatgtg gccacctgca ggtttgccac
     3421 tgcgcccccg ttggggatgt aacggacttc cggatcattg cccagacgcc cgacgaggat
     3481 gaccttgttg ataccacgtg cactcataag aacctcctga ctgaaagatg tgagataaac
     3541 cggataccgg cgacgggacg ttcatgtgcc ccttcagagg agcgaaagct cctcctgccg
     3601 tccacttcac aggggtttct ccccgcagtg gacctttccg atgggggccc gtttaccgcc
     3661 tcacgggaca gctgtccggg ggcggacgca aaatttttag ccgggttcat ggctaaaaat
     3721 tttgtggccg gtgcatctca ccctggacag atggaacggg agggggttac gtctgccccc
     3781 cggaaagtcc acagcgggaa acaggccgca ggggtggcgg caggagggaa gactgctgcg
     3841 ccatcaggcg cagtttcctc acacaccgtc aggtgtgcag ggagcgcagg tcacggcgcg
     3901 acaggggcgg cgcgaccgga acggtcgctg caagccgggt tttcacgcat cggaccgtag
     3961 cggcagcccg aaaccgcgcc agcgggttcg gcggagactg gcgggtgcgc cagcagctgc
     4021 ctgtcggagt cgggcctgac gaccggatgc cgccaggcag ccgggagccg aaggtgtccc
     4081 cgcctccgga aatcagaaca acagaaacga cataacagaa aaatgcctca caccatgacg
     4141 ggcaggaagg gcagcgccgc aggcgcggcc ctcctgcatt tcaggcaggc gtaagcctgc
     4201 cagggttttc cggcggcctg gcaggccata cccccgccgg agcgggggag taaatcaccg
     4261 acggaacaca tgaccgtcaa taaaggtgaa ggagtccagg aataaatccc gcgcatacgc
     4321 ctcatagtca aaatagagcg ccaccgtctc cggcacgtca gccagcaggc cggtatcact
     4381 ggcgaactca accgcaaaag cctcctcact gtcagcctcg ccccaccagg catcgcggaa
     4441 ggtgtcaaaa tccgtctcac cggtatcctc cacccagaga cgataagcct cttcgcagcc
     4501 ttcatcccgt gccaggcgga agccttcaac ccaggcccag ttgatatggc attcagaggc
     4561 catattcccc gggaatccct cataatcctg aaacatcagt tcaggatcgg cttcatcctg
     4621 gtgaagagca cggcaggcgg cgaaaaagtc gcgctcatca tcaaacgtgg tcaggtcaaa
     4681 ccagcgtccg gcgatgcttc cacagttgta tttgtgccag gttccaacgt atacagcagg
     4741 tgcaacaaca gacattcccg aatcctccat agtcacatgt tctgcgacac cgggcggtgc
     4801 cgtgcctcca taaaagccgg gcgataaggc ggggatgcag gggcgcaagg gaagccgcag
     4861 cctgacgcac acggctgacg ggagttgcag ggtgaggccg cgaaacctga gcggacgaac
     4921 gaccggaaaa tcacggcaga cgggaaggag ggaccgggtg accgcagcgg cgaagcgtac
     4981 ccttgcatcc ctgccagccc ggctaagatg gaggcacggc acagcccgca cacgacgctg
     5041 aggccacaga gctgaaaccc cacaaggcca tgccccgcat ggccgggtaa aagcggagcc
     5101 gccggcgact tccttattcc ggcgagtgca gaggacacgc cgcatgacct ccagaccacc
     5161 cctgatatga aacgatcatg atcggcgtgt ccctgcgtaa aatatacatg cctggttcac
     5221 cggagaatat ctgtgatatc cacaagtttt ctgtcgcgca gccagtcctg cgcttcacgc
     5281 accacacaac tgtccggttt caccgctttc agcgatgatg ccagtcgctt gatcaacatc
     5341 accagttccc gtgtctgtat gtccggcatt gtctccggcg gagcaggggg actctcattt
     5401 ccgacgctca tcccatgcgc ttccagtgtg gcacggacaa accaggcggc atcgaccagt
     5461 tgctgtccaa tccaggccag atcggcattt ctgtcacggg ctgccattcg ctcgcccgac
     5521 agcgccttgc tgatatggct gcgcaccaga ttatccagga gtgcctgcgc ttcttccggt
     5581 gaaaagccac ctggtatctc tgtgcgcaac agccctttcc tgtacagaag ctgcagcaga
     5641 tcaacctgcg accgcacagc aactatcagt acataacgga caacattcgc actggcagtg
     5701 gtttctgtat cattcagcag ttcttccgga tcgttccccc cggtgttgca gagcaacggc
     5761 aacagaggtc gcatccacgc gggaacctgg tatttttcgt ctgatttcat catattacgt
     5821 tctccagctt gttcgggatc atccactgct caagtgcacg tacctgtacc ggtgcaccac
     5881 gaccggcctc accggcttca aaaatcagcc gccagagtgg ttcctcctgc cagaaaaacc
     5941 ggtcaaatga tgacagtgat ggaaaacgcc cgcaccaccc ccagaaaatc gtttccagat
     6001 catgctcctc aacaacacca gaccagcggg acaaccagct tccgaccgtg gcgggagtat
     6061 gaaacgcaag gtcaatctgc gcctgagcaa aacatgtctg gtattccagt tcccgtcgtt
     6121 ctgcctcacg ttcccgctgg cggctgtatt tctgctctgt cagcgtggtt ttctgacgac
     6181 gacgctcacc ttcacgaaaa ggcacggacg ggaacagacg gtgccccgca tcggatggca
     6241 ggggaagaac acagacttcc ccaaaacttt caatcagttt atccacggca gccagccaca
     6301 ggagacggtt gccatgaaat tcctccttcg tcagcgcagg ggc
//
LOCUS       DAZFTN010000201         6220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFTN010000201 DAZFTN010000000
VERSION     DAZFTN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..1476
                     /locus_tag="ACHKV2_004846"
     CDS             16..1476
                     /locus_tag="ACHKV2_004846"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6942572.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1512..1715)
                     /gene="ydfZ"
                     /locus_tag="ACHKV2_004847"
     CDS             complement(1512..1715)
                     /gene="ydfZ"
                     /locus_tag="ACHKV2_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO6942573.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1892..2578)
                     /gene="rspR"
                     /locus_tag="ACHKV2_004848"
                     /gene_synonym="ydfH"
     CDS             complement(1892..2578)
                     /gene="rspR"
                     /locus_tag="ACHKV2_004848"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO6942574.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2667..3413)
                     /gene="ydfG"
                     /locus_tag="ACHKV2_004849"
     CDS             complement(2667..3413)
                     /gene="ydfG"
                     /locus_tag="ACHKV2_004849"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO6942575.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3550..5595
                     /gene="dcp"
                     /locus_tag="ACHKV2_004850"
     CDS             3550..5595
                     /gene="dcp"
                     /locus_tag="ACHKV2_004850"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO6942576.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5640..6158)
                     /locus_tag="ACHKV2_004851"
     CDS             complement(5640..6158)
                     /locus_tag="ACHKV2_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO6942577.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1539 a         1495 c         1590 g         1596 t
ORIGIN      
        1 atttaaggaa ttgttatggg aaataatttg ttatcagcaa aagcgacact ccctgtttat
       61 gatcgtaata acctggcccc aagaattgtt catttaggct ttggtgcatt tcaccgtgcg
      121 catcagggcg tgtatgccga tattcttgct acagaacatt tcagtgactg gggatattat
      181 gaggtcaact taatcggcgg cgaacagcaa attgccgatt tacatcagca agataatctt
      241 tataccgttg cggaaatgtc ggccgatgcg tggacggctc gcgtcgttgg cgtcgttaaa
      301 aaagccttgc acgtacagat agatggctta gaaaccgtgt tggctgcgat gtgtgaaccg
      361 caaatcgcga ttgtctctct gacaatcacc gaaaaagggt atttccactc tccggcgacc
      421 ggacagttaa tgctcgatca cccgatggtc gctgccgacg tgcaaaatcc ccaccagccg
      481 aaaacagcaa caggggtgat tgttgaggcg ctggctcgcc gtaaagcggc aggacttccc
      541 gcatttaccg tcatgtcatg tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt
      601 gtcacttcct acgcacaagc cgttgatgta aaactagcac aatggatcga agataacgtg
      661 actttcccat caacaatggt ggaccgtatt gtgcccgcag tgacagagga tacgctggcg
      721 aaaatcgaac agcttaccgg tgtgcgcgat cctgctggcg ttgcctgtga acctttccgc
      781 cagtgggtaa tcgaagataa ctttgttgcc ggacgtccgg aatgggaaaa agcgggagcc
      841 gaactggtta gtgatgtgct gccttatgaa gagatgaagt tgcgcatgct caacggcagt
      901 cattcattcc tggcgtatct gggttatctt gccggatatc agcacattaa tgactgtatg
      961 gaagatgaac attatcgtca tgcggcgtat gccttgatgt tgcaggaaca agcgccgacg
     1021 ctgaaagtgc agggcgttga tttgcaagat tacgctaacc gattaattgc acgctatagc
     1081 aacccggcgt tacgtcatcg aacctggcag attgcgatgg atggcagcca gaaattgcca
     1141 cagcggatgt tggattctgt tcgctggcat ctggcgcatg acagcaagtt cgatctgctg
     1201 gcgctgggcg tcgcgggttg gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg
     1261 atagaaatca gtgacccact gttacctgtt attcagaagg ctgtacaaag tagtgccgaa
     1321 gggaaagcgc gcgtccagtc attgctggcg attaaggcga tttttggtgg tgatttgcca
     1381 gacaatagct tgtttactgc aaaagtgacg gaagcgtact tgtctttatt agcgcatggt
     1441 gcgaaagcga ccgtggcgaa atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg
     1501 cattcacacg cttagttcat gccgagacga atcaggtcca gtggtgccag tttctcttca
     1561 caaccttcaa caactacggt ttttccgcgg cggatttgct cagcggtcag accttcagta
     1621 tcaatcgaca ggatcttgcc agtgtgaccg gtgccgctaa ccataacacg gctgccagtg
     1681 gtgattgcgt tacggttacg atcgtaggtc gtcataggtg ttttctcctt tctgatttac
     1741 agtcgaagcc cacgtaacgg gcgcaatata aatactcctg tcgagtgaaa tattttttgt
     1801 ttttgatcaa gctcacatct tttttgatgc atgcatgagc cataaggcgg gggaaaagac
     1861 tctcaccccg gatgggatga gaggaaatga attactcttc gctaaaccag tcgctatttt
     1921 cctggcggat ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc attgcacgtt
     1981 ctaccgcatc gccatcacgt ttttgcaggg cagagaatat atcaagatgc tggcgtaaca
     2041 gcatttctgg tggagaaaca tggtcgaaac tcatatagcg cacgcgatca acggtcgctt
     2101 tcaggttctc aatggtatcc cacgccagtt gacagtcggc aatctgcgtc aggagttgat
     2161 ggaagttgtc atcaagttca aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt
     2221 gctggtgaag attttgttcc agttggtagc actggctttc ggtaatcata ctcgccgccc
     2281 gacgcgccac cgcgcactca atggcctgac ggataaaact gccgttgcgc acctgggcca
     2341 tggaaatttt gttgacgtag ctgccacgct gcggacgaat ttgaatcagg ccgttttccg
     2401 ccagtttaat aaaggcttca cgaaccggct ggcgtgacac attgaaacga acagaaactt
     2461 ctttttccga caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa
     2521 tacgataaat ctgctgattg acaggctgtg tgggattaag ttgcgtttcg acggtcattc
     2581 gttcttgctt gtgagtgagt taactgcatg agtctaccac ttttttgtga caagcaataa
     2641 cccggcagtt acaccgggta taaaaattac tgacggtgga cattcagccc ggcatagctt
     2701 tgggtaaccg gcatcatttc cagggtattg atattgacgt gagcaggcag cgttgacacc
     2761 caccagacgg cttcgctgac atcttctggc gttaatgcaa cggtattttg ataggttttt
     2821 tccgctttac cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc acccaccaga
     2881 cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca ggtccgtacg cagattcagg
     2941 ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc atacggccag
     3001 ctacctgccg ttgagccaat gttaataata tgaccatgat tacgttcaac catacccggt
     3061 aagacggcgc gcgtcatata taccaggcct ttgttgttgg tatcaatcat cgtttcccag
     3121 tcttcaatgc tggctttatg cgcaggctcc atgcccaacg ccagaccggc attatttacc
     3181 aggatatcaa tattgcacca ctcggcagga agcgatgcca gcatctcttc aatagcggcg
     3241 cggttgcgaa cgtccagttg ggcgatgtac agattatctc ccagttcgtc ttttaactcc
     3301 tgcaaccgtt cctggcgacg gccagtggcg ataactttat gcccttgttg aataaaacga
     3361 cgagtaatgc attcaccaaa acctgccgtt gctccagtta ctaaaacgat catctcactg
     3421 ttcctcaacg cttttgtgtg tcaataacat agcacgtgat gcttaagagc gggtaatact
     3481 ggtttgcaag attgcactcc gttatcagta agcctacgct atggaacacc tcgcgttcag
     3541 gagaagaaaa tgacaacaat taatcctttc cttgtgcaaa gcacactgcc gtatctggct
     3601 cctcattttg atcaaattgc caatcatcac tatcgcccgg cattcgatga gggaatacag
     3661 caaaagcggg cagaaattgc tgccatcgcg cttaacccgc aaacacctga tttcaacaat
     3721 actattctgg cactggaaca aagcggagaa ttacttaccc gcgttaccag cgtctttttt
     3781 gcgatgactg cggcgcatac caatgatgaa ttacagcgtc ttgacgagca gttttccgct
     3841 gaactggcgg aactggctaa tgatatctat ctgaacggtg aattattcgc gcgggtagat
     3901 gctgtctggc aacgccgtga atctctgggg cttgatagtg aatccatccg cctggtggag
     3961 gtgattcatc aacgttttgt cctagccgga gccaaacttg cacaagctga taaagcaaaa
     4021 ttaaaagtac tgaatacaga agctgcgaca ctgaccagtc agtttaacca gcggttactg
     4081 gcagcaaata aatccggcgg tctggttgtg aacgatatcg cgcagctggc aggaatgagt
     4141 gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg
     4201 ctgattccgc tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg
     4261 acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat
     4321 acccgcgcta tcattcaacg tctggtggag atccgtgcac aacaggctac attactcggt
     4381 tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg caaaaacacc tgaagcagca
     4441 ctcaacttta tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc
     4501 tccatacagg cggttattga taagcagcag ggcgggttta gcgcgcagcc gtgggactgg
     4561 gcattttatg ccgaacaggt ccggcgagag aaatttgatc ttgatgaggc gcagctcaag
     4621 ccatattttg aattaaacac ggtgttgaat gaaggtgtat tctggaccgc gaatcagctc
     4681 ttcggtatta agtttgtcga acgttttgat attcctgtct accatcctga cgttcgggtg
     4741 tgggaaattt ttgatcataa tggcgtgggg ctggcgttat tttacggtga tttcttcgcc
     4801 cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc aacgcttaat
     4861 gaaacgcatc cggtaattta taacgtctgc aattatcaga aacccgctgc cggtgagcct
     4921 gcgttgttac tctgggatga tgtcataacc ttattccatg aatttggtca tacgctgcac
     4981 ggcctttttg cccgccagcg ttatgccacg ctctccggca ccaacacgcc gcgtgatttt
     5041 gtcgaatttc cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt attcgctcgc
     5101 tacgcccggc attatcagag cggggcagca atgcctgacg aactgcaaca gaaaatgcgt
     5161 aatgccagcc tgttcaacaa agggtatgag atgagcgaac tgcttagcgc cgcacttctc
     5221 gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa
     5281 ttgcgggcgc tggtggcaga aaatatggat cttcctgcta taccgccacg ctatcgcagc
     5341 agttattttg cccatatttt tggtggcgga tatgccgcag gttattacgc ttatctgtgg
     5401 acgcaaatgt tggccgatga tggttaccag tggtttgttg agcagggcgg attaacgcgt
     5461 gaaaatgggc tgcgttttcg cgaggcgatc ctttccagag gtaacagcga agatctggaa
     5521 cgcctgtatc gacaatggcg cggtaaggct cctcagatta tgccgatgct gcaacatcgt
     5581 ggcttgaaca tataaacacc accaatggct gtcctggaga tatcgccagg ccagcctggt
     5641 tatagcagta actgcagcag ctgggcgagg gcaaacctca ccgctaaagc taataccgtt
     5701 tcgcagtcgc cagcaaaatg cataaccgca gtgtagttct ggcctttaat atgccacgcg
     5761 aaccagacgg tacccgctgg cgtaccatct tcaccgccct ccggtccgcc gtagccggta
     5821 atggcaatac tgacatcagc atccgcacgc tctatggcac cggttgccat ttctgctgcc
     5881 actttctcac tcaccgcaga atatcgttca agagattgct ggcttacgct gaggattttc
     5941 atctttgcct gatcggtgaa agtaacaaag cctgcaccgt aaaatttggg tgtatcttca
     6001 gctgcacaca gggcgctagc caacttaccg ccggtgcatg attcggcggt agttaagcgt
     6061 aattgatccg cgataagtct ttgactcaac gcggatgtca ggttttcaat agtgtcggta
     6121 tctgcaagtt gtacaatttt atttctatta atgttcactg ttttttgtcc acgcttttat
     6181 atgtaaaggt gatttgcttt ttctgtatgc tcgtccaata
//
LOCUS       DAZFTN010000202         6210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFTN010000202 DAZFTN010000000
VERSION     DAZFTN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..99
                     /locus_tag="ACHKV2_004852"
     CDS             <1..99
                     /locus_tag="ACHKV2_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942578.1"
                     /translation="DLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(107..373)
                     /locus_tag="ACHKV2_004853"
                     /pseudo
     CDS             complement(107..373)
                     /locus_tag="ACHKV2_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            531..1715
                     /locus_tag="ACHKV2_004854"
     CDS             531..1715
                     /locus_tag="ACHKV2_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6942579.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            1715..2227
                     /locus_tag="ACHKV2_004855"
     CDS             1715..2227
                     /locus_tag="ACHKV2_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6942580.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2282..2647
                     /locus_tag="ACHKV2_004856"
     CDS             2282..2647
                     /locus_tag="ACHKV2_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942581.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2656..2811
                     /locus_tag="ACHKV2_004857"
     CDS             2656..2811
                     /locus_tag="ACHKV2_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6942582.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            2798..5601
                     /locus_tag="ACHKV2_004858"
                     /pseudo
     CDS             2798..5601
                     /locus_tag="ACHKV2_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5614..6102
                     /locus_tag="ACHKV2_004859"
     CDS             5614..6102
                     /locus_tag="ACHKV2_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942583.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
BASE COUNT         1461 a         1506 c         1830 g         1413 t
ORIGIN      
        1 gatctggttg ctgctcagct aaacaacaga ccgagaaaga cactgaagtt caaaacaccg
       61 aaagagataa ttgaaagggg tgttgcattg acagattgaa tctacaagtg cctttttctg
      121 tcagcggttc tcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt ggcggtagct
      181 gcaactgacc atctgcatca tccagcgtga tggtgagctg gtccgcctca aatccccggt
      241 tgtcggtcag tgacaggctc atcaggcgct ctgccacgcc tgacagcgtt ttaccctccg
      301 cgagaatatc aaaatccggc actttcacgt ggtctgtgcc ctgactgagc aattgcatgg
      361 tggtgtcggt catctgttcc ctccctgtgc ggcatggtcg catgtgcgtg cggagggggt
      421 tactgctttt tgttgtcgcc gtggcgggag aacggcgcag gggtgagatt acgcgcgtgg
      481 tgggtgatga ttgttgccga atcatttaac ggatacaagg ggctgaagct atgagtgaaa
      541 ctcgttttca tggtgcccgt gttacggaaa gtaccgacct ggtaacagcg attaatgatg
      601 ttgattccag tgttatcggt atcgtggcaa cggcagatga tgcggacgcg gagctgttcc
      661 cgctgaacaa gcccacactg ctgacccgcg tcaatgacgt gctgggaaaa tgcgggacta
      721 cggggacgct ttatcgtgcg cttaaggcta tcgcagacca ggtgagcaca aaagtgatcg
      781 tcgttcgcgt ggctgaacac aaagaagaag gcggtaagac gcaggatcaa ctggttatcg
      841 gtggttctga atctgacggc agctatacgg ggatgtatgc gctgcttgtt gcagagcagg
      901 atgaaagcat cggataccgt ccgcgtattc tggccgcgcc ggagctggac acggaggcgg
      961 taacaaaatc cctgtgcgtg attgcgggta aactgcgcgc gtttgtgtat gccacatgtc
     1021 atggttgtaa cacgatggct gaggcgatta cctaccgcca gaaattcaac gaacgtgagg
     1081 tgatgctctt atggcctgac ttcatcgcct acaacctgaa aagtggcaaa aacgaaacgt
     1141 tccccgcgcc tgcttatgcg tgcggccttc gtgcgtacat tgaccatgag cagggctggc
     1201 acaaatcgct gtccaacgtt ccggttaaaa atgtgcttgg gatgtcgagg catgtgttct
     1261 ggtcgttgca ggccgaagac agtgatgcca acagcctcaa caacaaagaa atcacgacca
     1321 ttattcgtcg caacgggttc cgcttctggg gcaaccgcac accggaaacg aacgcctaca
     1381 tctttgaggt gtatacccga accgcacagg tgctggctga ttcaattgcg gaagcgcagt
     1441 ttgaaaccat cgacagtcca ctgacacctg cgaacgtgaa ggatgttatc agtgccatca
     1501 gggcaaaact ggattcactg gttactgccg ggaaactgat tggcgcggag tgctggtatg
     1561 acatcgagga taacagcacc acgaatttac gtcaggggcg tgtgcgtatt cgctacaaat
     1621 atacgcccgt tcctccgctg gaagacatgg agctttacca gtcgtttact gatgaattct
     1681 ttggtcccgc atttgcggtg ctgggaggtg cctgatggct gtaccaaaac atcttcgctt
     1741 ttttacgctg tttgtggatg gtgaaaacga agtgggtaag gtgacgtccg tcaccctgcc
     1801 taaactgacg cgcaaaaccg acagctaccg gggtggtggc atgatgggtg cggtaagtat
     1861 tgacctcggc ctggacgact ccgcgcttga tgcgagcttt gtcatggggg gcgcagttcg
     1921 tgagctgttc ctgaagtatg gcggcacgat tgacggcacg ctactgcgtt ttgcggggga
     1981 atactacacc gatgatgaaa gcgacctgta tgaagtcgaa atgcgcggac gtgtgacgga
     2041 aattgatatg ggggaagcca aacagggcga agccacatca cacacttacg ccattaaaaa
     2101 cacctactac aagctgagtg ttaacgatcg cccgttgtgg gagattgacc tgctgaactt
     2161 catttaccgg aaggacggca aggacattgt gcccgatcgc atccgttccg cgcttgggct
     2221 tggctgataa gtaatatgca ggcggcgcag tgcgtcgcct ctgactgaaa ggagtttcct
     2281 gatgaaagag acgaaaaaca tcgataccga aaacacggta gttactgaca ctgtgaaaga
     2341 aaccagtgag cgtggtgtaa aacttaccca accaattgag cgaggcggcg aaaaaatcac
     2401 gtatgtggag atcaccgggg ctattgagca ggctggatct ctgcgagatt tgtcgctgtc
     2461 tgatgtgctg aatctgaaag cggaatccat gtttacgctg ctgtcacgcg tgacatcacc
     2521 gcgactggat gaagtgacga tcaaaaaaat ggcatcccgt gactttattc agttatgtgt
     2581 ggttgccgta aattttttga gcggtgcgga ctctggcggg aagaacgaac aggcgacgga
     2641 agcctgatca cggttgtgtg ctttgagcac atagaagact ttgtggcaga tattgccgtt
     2701 atttttaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt ggttagctgg
     2761 cgtgagcggg cggcacttcg cagcgggaat gcagacaatg aagactcttg atatccgggt
     2821 cgctttcagc gccgttgaca ggctgacccg acctgccgaa aacgcacgcc gcctgatggg
     2881 gcagtttggt gactccatcc agcgaacgca gggggcgatc aaaaatctcg agcgtcaggc
     2941 gcgatcattt gagcgcgccc gcgacgctgt cagtaaagcg gatgctggca tcgtgaaagc
     3001 acgacgccag cttaacgccc ttaatcagtt acaacgcacg ggtacagtgc tcagcgaaaa
     3061 acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg aatcgcgcac
     3121 acgggaaatt cagaaaatga gggaacttgg cggagagctg aaacgccacg gcatttccct
     3181 gacaggcagc gataacacca ttcagcaggc catcagacgc accgaacaat acaacaacca
     3241 gcttgaacgc gaacggcagg cgcttgcgcg tgtaacgcag gcgcgtgagc ggtattcgca
     3301 cgcgcaggaa accgcgggaa aactgaaaac aggtggtgcg ctggcaattg gtgcggcagc
     3361 ggcgggcggc tatgctgccg ggcgtttttt gcagcctgcg atcgggttcg gcaaagagat
     3421 gtcccgcgtt caggcactga cgcgaatcga caaaaacagc ccgcagttta aggcgctgcg
     3481 tgagcaggcg ttaaaacttg ctgaaacaca gtttactgcg agtgatgccg ccagtgggca
     3541 gagctttctg gcaatggctg gttttactcc gcaggccatt caggccgcat tgcccggtgt
     3601 tcttaatatg gcgctggcag gtggcgtcga actcggcgag acggctgata taggctccaa
     3661 tatcctcaca cagttcaacc tgacagccga tcaaatggac cgggtgggcg atacgctgac
     3721 agcagcattt acccggacca atactgattt acgcgcgctg ggcgaaacca tgaagtatac
     3781 cggtccggtt gccgcaaaac ttggtatcag tcttgaagaa gcggcggcca tggccgggat
     3841 gcttgccaat aatggtcttc gcggaagcga tgctggcacg gccatgcgcg caagtctgtc
     3901 ccgccttgca tcaccgccaa aagctgcggc tgatgcactg aaagagctgg gggtgtcagt
     3961 tgctgacgcc agaggcaaaa tgcgcccgat ggaggatgtg ctgcttgatc tctataaggc
     4021 gacacaaaaa tacggacagg tggaccaggt ctccttcttc aaggacatcg ccggagaaga
     4081 ggcgttcgtt ggtttgcaga cgcttgttgc ggcggctggt tcaggagagc tgcaaaaact
     4141 gaccagagaa ttgcaggggg caaggggaga ggccgatcgc gtcgcaaaag taatggctga
     4201 taatcttgat ggggacctga aaaatctcga cagcgcatgg gaaggtcttc gtattcgcat
     4261 cagtgatctg gttgacggtc cgctgcgttc tgtcacgcag tggctcacgc gggtgcttga
     4321 aaaaatcacc tcgctggcgc aggcccatcc ggtactgacg cgccagctac tgatagcagg
     4381 cggtgcgttg ctggcaatga ctgcaacgat tggctcgttg tcgctggtta ttggggtgct
     4441 ttacgggaag ctggccaccc tgcgtcttgg ttttgacatt cttacccggt caatgaatgt
     4501 cgtcagggtg ttacctgcgc tatggggaat ggtgacgggt tccgtttctt tactgggagg
     4561 cgttatcggg gcgttgttca gtccggttgg tcttatcgtg gctgcgcttg ccggagctgc
     4621 cgttcttatc tggaaatact gggatcctat cagggctttc tttgccgggg tgttcagcgg
     4681 gattatggaa aggctgaccc cgttgcgcga cacctttgaa cggtttggcc ctgtttttga
     4741 cgtaatcgga agcggttcag ccaggtgttt aactggttta aatcgctgct gtcaccgatg
     4801 gagtccagca aggaaacgct ggataaatgt accagtgctg gcgaggtatt cggtaacgtt
     4861 cttggtggtg cgttacagct tgttctgacg cctgcaaaaa tgttgctgga tacgctggcg
     4921 tggatacttg aaaaactcgg tgtgcttccg gatgaagcgg aaagggcgcg caagaaaatc
     4981 gaagacgcac aacgtgcggc cattcttcag gacaaggttg ctctgtttca gggagacatt
     5041 gcgaaaatca atccgccgaa gtctgcggga aatagcaatg gcaccggaga tgataaaccc
     5101 aaagacaaca aacctctcac agacagcaat accggcacgc tacgcagact cagcaaaatt
     5161 gctgataaca caggtaagct ggttgatgag acaaaaaaac gcattggccc cggcgatatt
     5221 gtctttaaga acctgccccg cgcacttgct gttcgtgggg agtggcagga gcggaagatt
     5281 gcgcaggtca gtaagcctgc ccccgcaatt aatatcacac ccgtggtccc ggctccgctg
     5341 cctccggcgc tggtccctgt tgttgcggcc agctcccgcc cggtggtgga ggccatacga
     5401 tcgccagtgg catcagttcc tgcaacttcc cgtaaccggg agcctgttgc ctccggattt
     5461 ggcggtgaaa ttcatgttca tctgcataac gttgttacgc agaatccccg cgaactggcg
     5521 aaactggtcg gcgaaatggt cagggcagaa atggaacggc gcgcccgtgc caggcgtggc
     5581 agtttttacg ataaagattg aggagtcatg gccatgatga tgatctacgg catgtttgtt
     5641 tttgagctgc gcacgttgcc gcatcagcag ttacagcaaa acaaaagctg gcgacatgtg
     5701 aaaaatgaac gcgttaaccg ttcagcaagc tggcagtata tcggtgcagg tgatgatcgc
     5761 atcgttcttt ctggtgttct ttatcctgag attactggtg gcgaagtgtc gctgtcgttg
     5821 ttgaccacgc aggcatatac aggacgtcct tggcctttga ttgatggcgt cgggcagatt
     5881 tacggcatgt atgtcctgac cggaacgaat acgacccgct ccgagtttga tcgctacggt
     5941 aaggcgaaaa agatagaatt ttcactgact cttgaacgct gtgatgagga tttgcgggag
     6001 cgcctgcaat cctcatcgtt cagcgatatg ctgtccggct tcaaagataa ggtgacatca
     6061 tcccttaata gcgcggccag ctcagttaag gggctgctct gatttaacgt atgtcgccaa
     6121 tttcctgatg aaggtgactg gcgacttgct gttgtatgtc cttctcagaa aattgttttt
     6181 gaataacaaa taacaggatt ttataatctc
//
LOCUS       DAZFTN010000203         6113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFTN010000203 DAZFTN010000000
VERSION     DAZFTN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..294
                     /locus_tag="ACHKV2_004860"
     CDS             <1..294
                     /locus_tag="ACHKV2_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6942584.1"
                     /translation="GYMGTSATMTSGEQSGAVIRGVFDDPENISYAGQGVRVEGSSPS
                     LFVRTDDVRQLRRGDTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            306..884
                     /locus_tag="ACHKV2_004861"
     CDS             306..884
                     /locus_tag="ACHKV2_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942585.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            881..1276
                     /locus_tag="ACHKV2_004862"
     CDS             881..1276
                     /locus_tag="ACHKV2_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6942586.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1284..2024
                     /locus_tag="ACHKV2_004863"
     CDS             1284..2024
                     /locus_tag="ACHKV2_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942587.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            2040..2462
                     /locus_tag="ACHKV2_004864"
     CDS             2040..2462
                     /locus_tag="ACHKV2_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6942588.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2444..2878
                     /locus_tag="ACHKV2_004865"
     CDS             2444..2878
                     /locus_tag="ACHKV2_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6942589.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2871..5420
                     /locus_tag="ACHKV2_004866"
     CDS             2871..5420
                     /locus_tag="ACHKV2_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6942590.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5417..5746
                     /locus_tag="ACHKV2_004867"
     CDS             5417..5746
                     /locus_tag="ACHKV2_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942591.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAELGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5746..>6113
                     /locus_tag="ACHKV2_004868"
     CDS             5746..>6113
                     /locus_tag="ACHKV2_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6942592.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1420 a         1499 c         1999 g         1195 t
ORIGIN      
        1 gggtacatgg gaacgtcagc caccatgaca tccggtgagc agtccggcgc agtaatacgt
       61 ggtgtttttg atgaccctga aaatatcagc tatgccggac agggcgtgcg tgttgaaggc
      121 tccagcccgt ccctgtttgt ccggactgat gatgtgcggc aactgcggcg tggagacacg
      181 ctgaccatcg gtgaggaaaa cttctggata gaccggattt cgacggatga tggcggaagc
      241 tgtcatctct ggcttgggcg gggcgtaccg cctgccgtta accgtcgtcg ctgaaggggg
      301 gatgtatggc cataaaaggt cttgagcagg ccgttgaaaa cctcagtcgt atcagcaaaa
      361 cggcggtgcc tggtgcgtca gcaatggcca ttaaccgcgt tgcttcatcc gcgatatcgc
      421 agtcggcgtc acaggttgcc cgtgagacaa gagtacgccg gaaactggta aaggaaaggg
      481 ccaggctgaa aagggccacg gttaaaaatc cgcaggccag aatcaaggtt aaccgggggg
      541 atttgcccgt aatcaagctg ggtaacgcgc gggttgtcct gtcccgacgc aggcgtcgta
      601 aaaaggggca gcgttcatcc ctgaaaggtg gcggcagcgt gcttgtggtg ggaaaccgtc
      661 gtattcccgg cgcgtttatt cagcaactga aaaatggccg gtggcatgtc atgcagcgtg
      721 tggccgggaa aaaccgttac cccattgatg tggtgaaaat cccgatggcg gtgccgctga
      781 ccacggcgtt taaacagaat attgagcgga tacggcgtga acgtcttccg aaagagctgg
      841 gctatgcgct gcagcatcaa ctgagaatgg taataaagcg atgaaacata ctgaactccg
      901 tgcagccgta ctggatgcac tggagaagca tgacaccggg gcgacgtttt ttgatggtcg
      961 ccccgctgtt tttgatgagg cggattttcc ggcagttgcc gtttatctca ccggcgctga
     1021 atacacgggc gaagagctgg acagcgatac ctggcaggcg gagctgcata tcgaagtttt
     1081 cctgcctgct caggtgccgg attcagagct ggatgcgtgg atggagtccc ggatttatcc
     1141 ggtgatgagc gatatcccgg cactatcaga tttgatcacc agtatggtgg ccagtggcta
     1201 tgactaccgg cgcgacgatg atgcgggcct gtggagttca gccgatctga cttatgtcat
     1261 tacctatgaa atgtgaggac gatatgccaa caccaaatcc tctggcaccg gtgaaagggg
     1321 ccgggaccac actgtgggtt tataacggga acggcgaccc atatgcaaac ccgctttcag
     1381 acaatgactg gtcgcgtctg gcaaaggtta aagacctgac gcccggcgaa ctgaccgctg
     1441 agtcctatga cgacagttat ctcgatgatg aagatgcgga ctgggccgcg accggacagg
     1501 ggcagaaatc cgctggagat accagcttca cgctggcgtg gatgcccgga gagcaggggc
     1561 agcaggcgct gctggcgtgg tttaatgaag gtgatacccg tgcctataaa atccgcttcc
     1621 cgaacggcac ggtcgatgtg ttccgcggct gggtcagcag tatcggtaag gcggtgacgg
     1681 cgaaggaagt gatcacccgc acggtgaaag tcaccaacgt gggacgtccg tcgatggcag
     1741 aagatcgcag cacggtaaca gcggcaaccg gcatgaccgt gacgcctgcc agcacctcgg
     1801 tggtgaaagg gcagagcacg acgctgaccg tggcattcca gccggaaggc gcaaccgaca
     1861 agagcttccg tgcggtgtct gcggataaaa caaaagccac cgtgtcggtc agtggtatga
     1921 ccatcaccgt gaaaggtgtt gctgcaggca aggtcaacat tccggtcgta tccggtaatg
     1981 gtgagtttgc tgcggttgca gaaatcaacg tcaccgccag ttaatccgga gaatcagcga
     2041 tgttcctgaa aaccgaatca tttgaacata acggcgtgac cgtcacgctt tctgaactgt
     2101 cagccctgca gcgtattgag catctcgccc tgatgaaacg gcaggcagaa caggcggagt
     2161 cagacagcaa ccggaagttt actgtggaag acgccatcag aaccggcgct tttgtggtgg
     2221 cgatgtccct gtggcataac catccgaaga agacgcagat gccgtccatg aatgaagccg
     2281 ttaaacagat tgagcaggaa gtgcttacca cctggcccgc ggaggcaatt tctcatgctg
     2341 aaaacgtggt gtaccggctg tccggtatgt atgagtttgt ggtgaatgat gcccctgaac
     2401 aggcagagga cgccgggcct gcagagcctg tttctgcggg aaagtgttcg acggtgagct
     2461 gagttttgcc ctgaaactgg cgcgcgagat ggggcgaccc gactggcgtg ccatgcttgc
     2521 cgggatgtca tccacggagt atgccgactg gcaccgcttt tacagtaccc attattttca
     2581 tgatgttctg ctggatatgc acttttccgg gctgacgtac accgtgctca gcctgttttt
     2641 cagcgatccg gatatgcatc cgctggattt cagtctgctg aaccggcgtg aggctgacga
     2701 agagcctgaa gatgatgtgc tgatgcagaa agcggcaggg cttgctggag gcgttcgttt
     2761 tggcccggac gggaatgaag ttatccccgc ttccccggat gtggcggaca tgacggagga
     2821 tgacgtaatg ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac
     2881 cggtaggcga tctggtcgtt gatttaagtc tggatgcggc cagatttgac gagcagatgg
     2941 ccagagtcag gcgtcatttt tccggtacgg aaagtgatgc gaaaaaaaca gcggcagtcg
     3001 ttgaacagtc gatgagccgg caggcgctgg ctgcacagaa agcgggaatt tccgtcgggc
     3061 agtataaagc cgccatgcgt atgctgcctg cgcagttcac tgacgtggcc acgcagcttg
     3121 caggtgggca aagtccgtgg ctgatcctgc tgcaacaggg ggggcaggtg aaggactcct
     3181 tcggcgggat gatccccatg ttccgggggc ttgccggtgc gatcaccctg ccgatggtgg
     3241 gggccacctc gctggcggtg gcgaccggtg cgctggcgta tgcctggtat cagggcaact
     3301 caaccctgtc cgatttcaac aaaacgctgg tcctttccgg caatcaggcc gggctgacgg
     3361 cagatcgtat gctggtcctg tccagagccg ggcaggcggc agggctgacg tttaaccaga
     3421 ccagcgagtc actcagcgca ctggttaagg cgggggtaag cggtgaggct cagattgcgt
     3481 ccatcagcca gagtgtggcg cgtttctcct ctgcatcagg cgtggaggtg gacaaggtcg
     3541 ctgaagcctt cgggaagctg accactgacc cgacgtcggg gctgacagcg atggcgcgcc
     3601 agttccataa cgtgacggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg
     3661 aagaagccgg ggcattgcag gcggcgaacg aggccgcgac gaaagggttt gatgaccaga
     3721 cccgccgcct gaaagagaac atgggcacgc tggaaacctg ggcagacagg acagcacggg
     3781 cattcaaatc catgtgggat gcggtgctgg atattggtcg tcctgatacc gcgcaggaga
     3841 tgctgattaa ggcagaggcc gcgtttaaga aagcggacga tatctggaat ctgcgcaagg
     3901 atgattattt tgttaacgat gaagcgcggg cgcgttactg ggatgatcgt gaaaaggccc
     3961 gtcttgcgct tgaagccgcc cgaaagaagg ctgagcagca gagtcaacag gacaaaaatg
     4021 cgcagcaaca gagcgatacc gaagcgtcac ggctgaaata taccgaagag gcgcagaagg
     4081 cttacgaacg cctgcagacg ccgctggaga aatataccgc ccgtcaggaa gaactgaata
     4141 aggcactgaa agacgggaaa atcctgcagg cagattacaa cacgctgatg gcggcggcga
     4201 aaaaggacta tgaagcgacg ctgaaaaagc cgaaacagtc cggcgtgaag gtgtctgcgg
     4261 gcgatcgtca ggaagacagt gctcatgctg ccctgctgac gcttcaggca gaactccgga
     4321 tgctggagaa gcatgccgga gcgaatgaga aaatcagcca gcagcgccgg gatttgtgga
     4381 aggcagaaag tcagttcgcg gtactggagg aggcggcaca acgtcgccag ctgtccgcac
     4441 aggagaaatc cctgctggcg cataaagacg agacgctgga gtacaaacgc cagctggctg
     4501 tacttggcga caaggtcacc tatcaggagc acctgaacgc gctggcacag caggcggata
     4561 aattcgcaca gcaacaacgg gcaaaacggg ccgccattga tgcaaaaaac cgggggctga
     4621 ctgaccggca ggcagcgcgg gaagccacgg aacagcgcct gaaggaacag tatggcgata
     4681 atcctctggc gctgaataac gtcatgtcag agcagaaaaa gacctgggca gctgaagacc
     4741 agcttcgcgg gagctggatg gcaggcctca ggtcaggctg gagtgagtgg gaagagagcg
     4801 ccacggacag tatgtcgcag gttaaaagtg ctgccacgca gacctttgat ggtattgcac
     4861 agaatatggc ggcgatgctg accggcagtg aacagaactg gcgcagcttc acccgttccg
     4921 tgctgtccat gatgacagaa attctgctta agcaggcaat ggtggggatt gtcggaagta
     4981 taggcagcgc cattggcggc ggcgcatcag cgtcaggcgg tacagccatt caggcagctg
     5041 cggcgaaatt ccattttgcg gccggagggt ttacgggaac cggcggcaaa tatgagccag
     5101 cggggattgt tcaccgtggt gaatttgtct tcacgaagga ggcaaccagc cggattggcg
     5161 taggaaatct ttaccggctg atgcgcggct atgccaccgg cggttatgtc ggtacaccgg
     5221 gcagcatggc ggacagccgg tcgcaggcgt ccgggacgtt tgagcagaat aaccatgtgg
     5281 tgattaacaa cgacggcacg aacggtcaga tagggccaca ggcactgaag gcggtttatg
     5341 acgtagcccg taaggcggca atggatgttg tgaccgggca gatgcgcgat ggtggtctgt
     5401 tctccggagg tggacgatga aaaccttccg ctggaaagtg aaacccggta tggatgtggc
     5461 ttcggcccct tccgtaagaa aggtgcgctt tggtgatggc tattcccagc gagcgcctgc
     5521 cgggctgaat gccgacctga aaacgtacag cgtgacgctt tctgttcccc gttgggaggc
     5581 cacggcgctt gagtcgtttc tggctgagct cgggggctgg aaggcctttc tgtggacgcc
     5641 gccttatgag tggcggcaga taaaggtgac ctgcgcaaaa tggtcgtcgc gggtcagtat
     5701 gctgcgtgtt gagttcagcg cagagtttga acaggtggtg aactgatgca ggatatccgg
     5761 caggaaacac tgaatgaatg cacccgtgcg gagcagtcgg ccagcgtggt gctctgggaa
     5821 atcgatctga cagaggtcgg tggagaacgt tattttttct gtaatgagca gaacgaaaaa
     5881 ggtgagccgg tcacctggca ggggcgacag tatcagccgt atcccattca ggggagtggt
     5941 tttgaactga atggcaaagg caccagtacg cgccccacgc tgacggtttc taacctgtac
     6001 ggtatggtca ccgggatggc ggaagatctg cagagtctgg tcggcggaac ggtggtccgg
     6061 cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cag
//
LOCUS       DAZFTN010000204         6052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFTN010000204 DAZFTN010000000
VERSION     DAZFTN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            223..741
                     /locus_tag="ACHKV2_004869"
     CDS             223..741
                     /locus_tag="ACHKV2_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6942593.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            951..3092
                     /locus_tag="ACHKV2_004870"
     CDS             951..3092
                     /locus_tag="ACHKV2_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6942594.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3168..>6052
                     /locus_tag="ACHKV2_004871"
     CDS             3168..>6052
                     /locus_tag="ACHKV2_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6942595.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1388 a         1601 c         1942 g         1121 t
ORIGIN      
        1 ttgggtatgg ctatccagat atatattatt aagttattgc tgaataagag atttaatgat
       61 tttaaaaata gtttttacga caaccgtaaa ttctagctcg gtttgtatgt aaaaaatact
      121 gaaaaatgat gcggttccgt gaagtgagct gcatctgcct gtctgggatt aacggcaggt
      181 cattgtaagt agtaattaac cacgttaaca aggagtttac aaatgccaac accgtgttat
      241 atctccatca ctggtcagac ccagggaaat attactgcag gtgcatttac cgctgattct
      301 gtcggcaaca tctatgtgca ggggcacgaa gacgagatgc tggtgcagga attcctgcat
      361 aacgttactg tcccgaccga tcctcagtcc ggtcagcctg cgggtcagcg tgctcacaag
      421 cctttcattt ttaccgtggc gctgaacaaa gcggttccgc tgatgtacaa cgcactggcc
      481 tccggcgaaa tgctgccaac cactgaactt cactggtggc gtacctctgt ggaaggcaaa
      541 caggagcatt atttcactac ccgcctgact gattcgacga ttgtcgatat gaaactgcat
      601 atgccgcatt gccaggaccc ggcgaaacgc gagttcaccc agttgctcga agtttcactg
      661 gcctaccgca aaattgaatg ggaacacgtc aaatctggca cctccggtgc cgacgactgg
      721 cgcgcaccgc tggaagcata agttaagcca acagcatccg gctatcaggc cggatgctgt
      781 ttttgtatta cgccgtagaa agaaaggttg tcagtaaggg gttacgttca cgggagatgg
      841 cggctgccgg aaacttcggc ggtagcgtgc ctgaacgcaa gcgtagcact gtactgtcac
      901 agccgcttta acagcgaact gtctgttttt ccttaacccc ggaggttgct atgtcaaccg
      961 gattacgttt cacgctggaa gtggacggcc tgccaccgga tgcttttgcg gtggtctcct
     1021 ttcatctgaa ccagtcactc tcttcgcttt tttccctcgc tctctctctg gtcagccagc
     1081 agtttctctc ccttgaattc cagcagatcc tcgacaaaat ggcctacctg acgatatggc
     1141 agggcgatga cgtacagcgc cgggtgaaag gtgtggtgac ctggtttgaa ctgggggaga
     1201 acgacaaaaa ccagaagctg tacagcatga aggtatgccc gccgctgtgg cgcacagggc
     1261 tgcgccagaa cttccgtatc ttccagaatg aggacatcgc aagcatcctc ggcacgatat
     1321 tgcaggaaaa cggggtgacc gagtggagcc cgctgttcag cgagccacat ccttcccgtg
     1381 agttttgtgt ccagtacggt gagactgatt acgatttcct gtgccggatg gcggcggagg
     1441 aaggcatctt cttttatgag gagcacgcgc aaaaaagtac cgaccagagc ctggtcctgt
     1501 gcgataccgt gctttatctg ccggagtcct ttgagatccc ctggaatccg aacacccgta
     1561 ccgaggtaag caccctctgc atcagccagt ttcgctacag cgcacaaatc cgcccttctt
     1621 ccgtggtgac caaagactac acctttaaac gccccggctg ggccggacgt tttgaccagg
     1681 aaggccagca ccaggattac cagcgcacac agtatgaagt gtatgactat cccggacgtt
     1741 tcaagggcgc ccacgggcag aactttgccc gctggcagat ggacggctgg cgaaacaacg
     1801 cagaagtggc gcgcggaaca agccgttcgc cggagatatg gccgggacgg cgaattgtgc
     1861 tgacggggca tccgcaggcg aacctgaacc gggaatggca ggtggtggca agtgaactgc
     1921 acggtgagca gccgcaggcg gtgccgggac gcaggggttc aggcaccacg ctggataacc
     1981 attttgcggt aataccggca gacagaacat ggcgaccaca gccgttgctg aaaccgctgg
     2041 tggacggccc gcagagcgcc gtcgtgacgg gaccggcagg cgaggaaatc ttctgtgatg
     2101 aacatggccg cgtgcgggtg aaatttaact gggaccgcta taacccgtca aaccaggaca
     2161 gttcatgctg gatccgtgtg gcacaggcgt gggcaggcac cgggtttggt aacctggcga
     2221 taccgcgtgt gggtcaggag gtcattgtgg acttcctcaa cggcgatccg gaccagccga
     2281 tcattatggg gcgtacctac caccaggaaa accgcacacc cggtagcctg ccggggacga
     2341 agacgcagat gaccattcgt tcgaaaacct ataagggcag cgggtttaat gaactgaagt
     2401 ttgacgatgc gacagggaaa gaacaggtct acatccacgc gcagaagaac atgaacaccg
     2461 aggtgctgaa taaccgcacc acggatgtga taaacaacca tgccgaaaaa ataggtaaca
     2521 accaggcgat caccgttacc aataaccaga tcctgaacat tggcgttaat cagatacaga
     2581 cggttggtgt caaccaggtg gaaacggtag gcagtaacca gattatcaaa gtgggatcaa
     2641 accaggttga aaaggtgggg atcattcgtg cgctgacggt gggtgtggcg tatcagacga
     2701 cggtaggcgg cattatgaat acctcggtgg cgttgttgca gtcctcacag gtagggctgc
     2761 ataaatcact gatggtggga atgggctaca gcgtcaatgt agggaataac gtcaccttct
     2821 cggtgggcaa gacgatgaag gaaaacaccg gacaaacagc agtttattct gccggtgagc
     2881 atcttgaact ctgctgtggt aaggcaaggc tggtgctgac gaaggacgga agcatatttc
     2941 ttaatggtac gcacattcat ctggaagggg agtcggatgt gaacggtgat gcgccggtga
     3001 ttaactggaa ctgtggtgcc acacaacctg taccggatgc gcctgtgccg aaagatttac
     3061 cccccggaat gccggatatg cggcaatttt gagtcaggtg caaaaaatca aaacggaact
     3121 ggtgcagtga ccagataaac catattaaaa gcagaaggaa ttgattcatg ggaggaaaac
     3181 cggcggcgcg gcagggtgac atgacccgca agggactgga tattgtgcag ggttcagcag
     3241 gggtgctgat aggtgcgccg acgggcgtgg cctgctcggt gtgtccggga gggattacct
     3301 atgctaaccc ggtgaacccg gtgctgggtg cgaaggtgct gccgggcgag acggaccttg
     3361 cgctgcccgg cccgctgccg tttattcttt cccgcgccta cagcagctac cggaccagaa
     3421 cacccgcgcc ggtgggggtg tttggtcccg gctggaaagc gcccttcgat atccgcttac
     3481 agatacgcga tgaaggcctg atactcaacg acaacggcgg caggagcatt cactttgagc
     3541 cgctgtttcc cggcgagata agctacagcc gcagcgagtc gttctggctg gcgcggggcg
     3601 gggtggcgga gcagcacagt tcgcagccgc taagcgcgct ctggcaggtg ctgccggaag
     3661 atgttcgcct gagtccgcat atgtacctgg cgacaaacag ccttcagggg ccgtggtgga
     3721 tactcaactg gccggagcgg gtgccggggg cggacgaggt gctgccgccg gagccgcccg
     3781 cataccgggt gctgacgggc gtggtggatg gcttcgggcg gacgctggcc tttcaccggg
     3841 cggcggaggg tgatgtggcg ggcgcggtga cgggggtgac ggacggcgcg ggccgccgtt
     3901 ttcacctggt gctgaccaca caggcacagc gggcggaagt attccgtaaa cagcgcgcca
     3961 cgtctttatc ttcccctgcc ggtccccgtt ctgcttcctc ttctctggtt ttccccgaca
     4021 cgctgcccgc cggtacagaa tacggtgccg ataacggtat ccggctggag gcggtatggc
     4081 tgacacacga cccggcatac ccggatgaac tgcccgccgc gccgctggcg cgctacacgt
     4141 acacggccag cggagaactg cgggcggtgt atgaccgcag cgggacgcag gtgcgcgggt
     4201 ttgcttatga tgcggagcac gccgggcgga tggtggcgca ccattatgcg ggtaggccgg
     4261 agagccgcta ccggtatgat gataccggcc gggtgacgga gctggtcaac ccggaggggc
     4321 tggactaccg ctttgagtac gggcaggacc gtgtgaccat cacggacagc ctgaaccggc
     4381 gggaggtgct gtacacggaa ggcgagggtg gcctgaaacg tgttgtgaag aaggaacatg
     4441 cggacgggag catcacccgc agcgagtatg atgaggcggg gaggctgaag gcacagacgg
     4501 atgcggcggg acggcggacg gagtacagcc tgcatatggc gtcgggtgcg gtgacagcgg
     4561 tgacggggcc ggacggcagg acggtgcggt atggctataa cagccagcgg caggtgacgt
     4621 cagtgacgta cccggacggg ctgcgcagca gccgggagta tgatgagaag ggaaggctgg
     4681 cggcggagac ctcgcgcagc ggagagacaa cgcggtacag ctatgatgac ccggcgagtg
     4741 agctgccgac agggatacag gacgcgacgg gcagtacaaa acagatggca tggagccgtt
     4801 acggtcagct gctgaccttt acggactgct cggggtacac gacgcggtat gagtatgacc
     4861 ggtacggtca acaaatcgcc gttcaccggg aagaaggcat cagcacttac agcagttata
     4921 acccgcgtgg ccaactggtc agtcagaagg atgcgcaggg gcgtgaaacc cgttatgagt
     4981 acagcgccgc aggcgacctc accgctatcg ttgccccgga cggcagccgc agtgagatac
     5041 agtatgatgc gtggggaaag gccgtcagca ccacgcaggg cggtctgacg cgcagcatgg
     5101 ggtatgacgc tgccgggcgc atcaccgtgc tgaccaacga gaacggcagc cagtccacgt
     5161 tccggtatga cccggtggac aggctgactg aacagcgcgg ttttgatggc cggacgcaac
     5221 gttaccacta tgacctgacc ggaaaactca cgcagagtga agacgagggg cttgtcaccc
     5281 tctggcacta cgatgcgtcg gaccgcatca cgcaccggac ggtgaacggc gacccggcag
     5341 agcagtggca gtatgatgag cacgggtggc taaccaccct cagccatacc agtgaaggcc
     5401 accgggtgtc ggtccactac ggctatgacg ataaaggccg cctgacgggt gaacggcaga
     5461 cggtggagaa cccggagacg ggggagatgc tgtgggagca tgagacgggg cacgcgtaca
     5521 gcgaacaggg gctggcgacc cgtcaggagc cggacggtct gccgccggta gagtggctga
     5581 cgtatggcag cggttatctt gcggggatga agctgggcgg aacgccactg gtcgagtaca
     5641 tgcgggaccg gctgcaccgt gagacggccc gcagcttcgg cggggaggca tatgaacttg
     5701 ccaccgcctg gaataccagc ggccagctcc ggagcaggca cctgaacctt ccgcagcttg
     5761 accgtgacta cgactggaac gacaacggac agctaatccg catcagcggc ccgcaggaga
     5821 gccgggagta ccgttacagt gacacgggaa ggctgacggg cgtccacacc acggcagcga
     5881 acctggatat cgatatcccg tatgcaacgg acccggcagg aaaccggctg ccggacccgg
     5941 aactgcatcc ggacagcacg ctcacggcgt ggccggataa ccgcatcgcg gaagatgcgc
     6001 actatatcta tcgctacgat gaatacggca ggctggcgga gaagacggac cg
//
LOCUS       DAZFTN010000205         5975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFTN010000205 DAZFTN010000000
VERSION     DAZFTN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..242
                     /locus_tag="ACHKV2_004872"
     CDS             108..242
                     /locus_tag="ACHKV2_004872"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942596.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            844..1665
                     /locus_tag="ACHKV2_004873"
     CDS             844..1665
                     /locus_tag="ACHKV2_004873"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6942597.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1665..2771
                     /locus_tag="ACHKV2_004874"
     CDS             1665..2771
                     /locus_tag="ACHKV2_004874"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6942598.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2861..4582
                     /gene="cptA"
                     /locus_tag="ACHKV2_004875"
     CDS             2861..4582
                     /gene="cptA"
                     /locus_tag="ACHKV2_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6942599.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4656..5654
                     /gene="lpxM"
                     /locus_tag="ACHKV2_004876"
                     /gene_synonym="msbB"
     CDS             4656..5654
                     /gene="lpxM"
                     /locus_tag="ACHKV2_004876"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6942600.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5707..5895
                     /locus_tag="ACHKV2_004877"
                     /pseudo
     CDS             5707..5895
                     /locus_tag="ACHKV2_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1529 a         1307 c         1504 g         1635 t
ORIGIN      
        1 cagcttttta aactaatatc gactttcaat aaataaggac aggtaatgga caacaaaaat
       61 atctgaaccc gggtgagatt cataagttgc acagtgctgt cttcaaagtg ccacacccgg
      121 aacgtaatca ctgcctgctc cttatggggt atcttcatgg tgtccaagca tcagagttat
      181 taggtataaa actgtctgat attgatttgc aggccggtaa cctgaatatc cggcggctct
      241 aaacggtttg tcaactcagc ccctcggctg aaacaggagg tacagtccat tcgtcgctgg
      301 ctgaaacttc gggatgaatg ggtagaagag ggatatgaat ggttatttaa cctctatggc
      361 aggtctgcta caggtattac tgttgttctg gggggcactg gtgtggttaa tatctacgat
      421 gcaagagatt ctcacgatgg cccggtccat cagtgcatgc ggcgcgatat tgtgcgagta
      481 tttttcaggg ggagtggtat ccggatgcag aaaattccgc tactcgactg gttttatgca
      541 ataaatcagg atgataatct tctgattttt tactgaggcg gatatctctc gctgtgttta
      601 ctgaaattgc ggtattctgt tttttctgaa aaacagaaaa tctgattaaa aagagcatat
      661 gcgttaaggg aaatttaagt tctggttgat taactgataa aaatattaaa aaatccctgt
      721 tgtcggtaat ctccggacca ttccgggatt acacaataat atcaccatgt gtgatatcta
      781 tggatcctca ctgttgttta cgcgaggata ttttcagaat gcttaacaga cgattatttt
      841 tttatgttga atgcaaggca tcttcccgta ctgatgtatc atcatgtcag tcgttgtccg
      901 gggcttgtga cactctcacc tgaaacattt tgtgaacaga tgaagtggct cgcagataat
      961 aactggcgga cggtaacatc tgctgagctt gaatattttt atcagggggg aacgcttcca
     1021 cgaaagagtg tcatgctcac attcgatgat ggttatcttg ataactggct cagagcatgg
     1081 ccggtcctga aggaatataa ccttcatgca catatttttc tcattacggg gctgattggt
     1141 aagggaaacg tacgtagcag gcaggagcag gaatattctc accgcgactg tgaacggttg
     1201 attgccgata accgttcaga tgaggtcatg cttcgctggt ctgaagtccg ggaaatgcgt
     1261 gattccggac tggttgaatt tcatgtgcac acccattcac ataaacgctg ggacaggttg
     1321 tctgtatcac gtgctgaaca gtgccggctc atgaaagaag atattctggt gggaaaacaa
     1381 tgtctgacgg aaaagctggg gttctgtagt tcccatctgt gctggcctga aggatattat
     1441 aatcgggatt atattaatct cgccggtaag cttggtttct cttatttata tacaaccgaa
     1501 agaagaatga actgtcctga aaatggttca ttacgcatag ggcgtatcag caccaaagag
     1561 cgggaacatt ccggctggct gaaacgacgc cttttttatt atacaacgcc acttttttct
     1621 tcagtactgg ctcttcataa ggggccaaga ttaccagata attaatgatg aaaattttat
     1681 tcactgaatc ttcgtcagat attgggggcc aggagctgca ggcactggcc cagatgactg
     1741 ctcttcaaaa gcagggacat tccgttttac ttgcctgcag agaaaaaagt aaaattgctc
     1801 cggaagccag aaaaagaggg catgatgtca cttttattcc tttcaggaac agccttcacc
     1861 ttccttccat cctcagactt cgacggatta tcggggaatt taaacctgat ctggtcatct
     1921 gccatagtgg ccatgacagt aatatagcag gactgagccg tctgatatgc tgtcaccgct
     1981 tcagtattgt cagacaaaaa acctacatca cccgtaagac cagaacgttt tccctgaatt
     2041 atctgtgtga tttcattgtt gtgcctagct cagccatgat ggcgcatctg atggcggaag
     2101 gtgtcaggac accggtaacg gttattccac cgggtttcga ctggcccgca ttgcataatg
     2161 aagccatgcg accgttaccc ctgcatatcc acgcctgggc tgcatcagca gacaatgttc
     2221 cgcttatcgt tcaggtgggg atgctacgtc ccgaaaaagg acatgaattt atgctgcgtg
     2281 tattgtatca gctgaagatg gaagggaaat catttcgctg gctcgtggtg ggcgccgggc
     2341 gtgaagaata cgaagcacgt ctgcgtcagc agacagaaca cctgggaatg agcggagatg
     2401 tgttaatggc aggtgccctt ttcccagcgt taccggtcta ccggattgcc agtgtggtgg
     2461 tcatgccttc ggaaaatgaa gcgtttggta tggtactggc tgaagcgtca gtctccggcg
     2521 tgcccgttat cgccagtgag acgggcggga tccctgatgt cattcagaag aatgtgacag
     2581 ggacgctgtt acccgtgggg gatgtgtctg catggaccgg cgcactgcgc gattttttat
     2641 cccgaccgga acgcttccgg atgatggcag ccagcgccag agaagatatt gagtaccggt
     2701 ttgatattaa cagaacggca cagatcattg tgtctctggc tagtcaggct aaggggaaat
     2761 gcaacagata acggcggatc ctcttttcag cccgaatttt gttcagcggt tttccggtaa
     2821 cggagtaatt tatcacattg tttaaatacg gggacggtca atgcatttaa ataccggaca
     2881 gaacaggcct actttcagct ggagcgcgct gggatgggca attttttatt ttggcttttt
     2941 ttccactctc ctgcaggtca tcatcttcag cagtgggtac agcggaacga atggaatacg
     3001 ggactcactg ttattcagtt gtctgtggtt gatcccggtg tttctctatc ctgatcggat
     3061 aaaaataatt gcagctgttg tcggtttcat tctctggggc acgtcgctgg cagcactttg
     3121 ttattatttt ctctatggtc atgaattctc tcaaagtgtt cttttcgtta tgtttgaaac
     3181 gaatgccaga gaggctggtg aatatttcag ccagtatttc agccttaaac tgttgcttat
     3241 ctcgctggta tatactgcgg tgtccgtttt tctgtggaca cgtctgcgtc ctgtatatat
     3301 tcctttgcca tggcggagaa ttgtctcttt cctgctgctt tatgctctgc ttctgcatcc
     3361 ggttgttctg aaatcgttaa tcagacagga gccgctgaat gatactctag gcaaactggc
     3421 atcccgaatg gagcctgctg ccccctggca gtttgtatcc agttattatc agtaccatca
     3481 gcaactgaat gcactgacaa ccttcctgaa tgaaaatagc gcactgccac cactgggtaa
     3541 tctcagggat gaatcagggg agagaccacg cacactggtc ctggtgattg gtgagtcgac
     3601 acagcgcgaa cgcatgagct tatacgggta tctacgtgaa acgacgccgg agctggatgc
     3661 actgcgtaaa accgatccgg gtcttactgt gtttaataat gtggtggcat cgcgtccgta
     3721 caccattgaa gcattgcaac aggcccttac tttcgccaac gaaaagaatc ctgatctgta
     3781 tctgacgcag ccgtcgctga tgaacatgat gaagcaggca ggctataaaa cattctggat
     3841 taccaaccag cagacaatca cagcccgtaa caccatgctc actgtatttt cgcgccagac
     3901 ggacaggcag tactacatga atcagcagcg aacacaaagt gcgcgtgaat atgacactaa
     3961 cgtgctgaag ccgttccggg aagtgctgaa tgaccctgca ccaaagaaac tgatcatcgt
     4021 gcatctgctg ggtacgcaca ttaagtataa ataccgttat ccggaaggtc aggggcggtt
     4081 tgatggcatt acagggcata ttcccactgg attaaatgcg aaagagctgg aagtgtataa
     4141 cgattacgat aatgccaatc tgtttaacga tcatgtggtg gccagtctga taaaggactt
     4201 cagagcaact gcgccggacg gctttctgct ttatttttca gaccatggtg aagaagtata
     4261 tgatactccg ccatataaaa cgcagggacg gaatgaagat aaccccacac gtcccatgta
     4321 cactgttccg ttcctgctgt ggacctcgga aaagtggcat gctgcacatc cgcgagattt
     4381 ttcgcagtat gttgaccgca aatacagtct ggctgaactg atccacacct ggtcagattt
     4441 ggcgggactg acatacgatg gttacgatcc gacccgttct ctggtgaatc cgcaattcag
     4501 ggaaaccacc cgctggattg gaaatccgta taaaaagaat gggctcactg atttcgacac
     4561 tcttccgtat ggtgagccat agtattttct tatcttcggt tccgttctga aggcaggagg
     4621 atgtgttttc ctctgctgtc cggtggcctc cagggatggt accaccttct gctgtactct
     4681 gttatcataa tgagataagc aggcagatac cggtaaatat gaaaaatatc agaactgagt
     4741 ttattccccg ctttaacctt acattatgct tcccccgtta ctggatgacg tggacaggta
     4801 tcggtattat ctgtgtgttt gcaatggtcc cccccgcact gagagatcca ttgttgggaa
     4861 aattgggcat gctggttggg cgtctgggga aaagcgcacg tcaacgggcc ttgattaatt
     4921 tatcgctttg ttttccggag tacagtgata aagaaaagga gaatatcgtt gatgccatgt
     4981 ttgcaacagc ctccatggcc gttgtgctta tggctgaatt agcactgtcc gggcctgata
     5041 aaatttcgca tcgtatacgc tggaatggtc tggagattgt ggagaagatg gcacagaaca
     5101 atgaaaaggt catttttctt gtgccacatg cctggggagt ggatattccc gcaatgctga
     5161 tggctgcgtc gggcagaaaa atggcagcga tgtttcataa ccagagaaat ccggtggtgg
     5221 attatgtctg gaacagtgtt cgtcgtcgtt tcggaggaaa actgcacgca cgtaatgacg
     5281 gtattgcctc ctttgtccgc tcagtccggc agggatactg gggatattat cttcctgatc
     5341 aggatcatgg cccggaattc agtgagtttg cagatttttt tgcgacgtat aaagcaactt
     5401 taccggttat cggacggctc agcaggatca gtggtgcacg gataataccg cttttccctg
     5461 tttatgacgg aaagacccat catctgacta ttcatgtcag cccgccactt gctatacgac
     5521 agaagagtga tgctcatatt gcgcgacaga taaacgaagt ggttgaaaat ttcgttcgtc
     5581 cacatcctga gcagtatacg tggatcctca agttgctgaa aacgcgtaaa gaaggggaag
     5641 aggatcctta ctgaggcagc tttttcagga taatgagctt acccagcaat agtggacaca
     5701 cgctaagtga gtaaactctc agtcagaggt ggctcatatg acaaaaaaac agtaccaacc
     5761 agtaaaaaac cccgtaaata gcattcgcct gaatttcgca gtgaagccct gaagcttgct
     5821 gaacgcatcg gtgttgctgt cgcagctcgt gtgctcagtc tgtatgaatc acagcttgta
     5881 agcggctcgt cagaactgta ttgatattta ctgagcggtc agttcaacgt aagcgagtgt
     5941 tatgcgccat ggaagcttac ccaggaatat gttat
//
LOCUS       DAZFTN010000206         5806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFTN010000206 DAZFTN010000000
VERSION     DAZFTN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2667
                     /locus_tag="ACHKV2_004878"
     CDS             <1..2667
                     /locus_tag="ACHKV2_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6942601.1"
                     /translation="GTLNDFLGAMTEDDVRPEALRRFELMVEEVARNASAVAQNTAAA
                     KKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASK
                     SAAAAESSKSAAATSAGAAKTSETNAAVSQQSAATSASTATTKASEAASSARDASASK
                     EAAKSSETSAASSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASAAAGSKTA
                     AALSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAK
                     TSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTK
                     ATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSAT
                     NSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNSTSEMLAATPKSV
                     KAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSA
                     RKVNGKALSSDITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGA
                     GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAI
                     GNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTG
                     ASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVA
                     IGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2667..3248
                     /locus_tag="ACHKV2_004879"
     CDS             2667..3248
                     /locus_tag="ACHKV2_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6942602.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3368..4258)
                     /locus_tag="ACHKV2_004880"
     CDS             complement(3368..4258)
                     /locus_tag="ACHKV2_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFO6942603.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(4277..4783)
                     /locus_tag="ACHKV2_004881"
     CDS             complement(4277..4783)
                     /locus_tag="ACHKV2_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6942604.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(4820..5320)
                     /locus_tag="ACHKV2_004882"
     CDS             complement(4820..5320)
                     /locus_tag="ACHKV2_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6942605.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(5399..5581)
                     /locus_tag="ACHKV2_004883"
     CDS             complement(5399..5581)
                     /locus_tag="ACHKV2_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6942606.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1571 a         1373 c         1389 g         1473 t
ORIGIN      
        1 ggtacgctga atgattttct tggtgccatg actgaggatg atgtccgtcc ggaggcactg
       61 cgccgttttg agctgatggt ggaagaggtg gcgcgtaacg cgtccgcggt ggcacagaac
      121 acggcagccg cgaagaagtc agccagcgat gccagcacat cagcccgtga ggcggcaacc
      181 catgcgactg atgctgcgga ctcagcacgc gcagccagca cgtcagccgg acaggccgcg
      241 tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat caacaaaggc tactgaagca
      301 tcaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg cggctaccag tgccggtgcg
      361 gcgaaaacgt cagaaacgaa tgcggcagtg tcacaacaat cagccgccac ttctgcatcc
      421 accgcgacca cgaaagcgtc agaagctgcc tcctcagcca gggatgcgtc ggcttcaaaa
      481 gaggcggcaa aatcatcaga aacgagcgca gcctcgagcg ccagtagtgc agcctcctcg
      541 gcaacggcgg caggcaattc cgcgaaggcg gccaaaacgt ctgagacaaa cgctaagtcc
      601 tctgaaacgg cagcagaaca gagtgcctcc gcagcagcag gctcaaaaac agcggctgca
      661 ttatctgcca gtgccgcgtc aacaagtgcc gggcaggcct cagccagtgc caccgccgcc
      721 ggaaaatcgg cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa ggctggcgaa
      781 gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac atccgaaacg
      841 aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc atcgtcagcc
      901 agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc gaccagacaa
      961 gcgtcagcag cgaagagcag cgccacgacg gcatccacga aggcgacaga ggcagctggt
     1021 agtgcgacgg cagcagctca gagcaaaagt acggcggaat ctgcagcaac gcgcgctgag
     1081 acagcggcaa aacgggcaga ggatattgca tccgccgtgg cgcttgagga tgcgagcacg
     1141 acgaaaaagg ggatagtaca gctcagcagt gcgactaaca gcacttccga gtcactggcg
     1201 gcaacgccaa aagccgttaa ggccgcgtat gagctggcta acgggaaata caccgcacag
     1261 gatgcaacga cagcacagaa agggatagtt cagcttagca acgcgaccaa cagcacatct
     1321 gaaatgctgg cggcaacgcc aaagtcggta aaggcagcct atgaccttgc taacgggaaa
     1381 tatactgctc aggacgctac gacagcacaa aaaggaattg tccagctcag tagtgcaacc
     1441 aacagcgcat ctgaaacgct tgccgcgaca ccgaaagcag tgaaagcagc taatgataat
     1501 gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta aggcgctttc atcggatata
     1561 acactgacgc cgaaagatat tggtacgctt aactcaacaa caatgtcatt cagcggtggt
     1621 gctggttggt tcaaattagc aacggtaacc atgccacagg cgagttctgt tgtttcaatt
     1681 acgttgattg gtggcgcggg atttaacgtg gggtcacctc aacaggcagg tatatctgaa
     1741 cttgttttgc gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt atggcagcgc
     1801 acatcgacag ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac ttacgatatt
     1861 tacgttgcaa tcggaaatta tgcgactggt gtaaatattc aatgggatta taccagtaat
     1921 gccagcgtga cgattcatac gtcaccagca tattctgcta ataagccgga agggttaacg
     1981 gacggtacag tttattcact ctatacgcca tcagagcagt tttatccgcc tggcgcacca
     2041 atcccgtggc catcagatac cgttccgtct ggctatgccc tgatgcaggg gcagactttt
     2101 gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
     2161 cgtggctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
     2221 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     2281 accacatcgt cgtttgatta cggcactaaa tccacgaata acaccggggc gcacacgcac
     2341 agtgtgagcg gtacagccgc aagtgccgga aaccatactc atagtgtcac aggcgcatca
     2401 gcagtcagcc agtggtcaca aaatgggtca gtacataagg tagtgtctgc ggccagtgtg
     2461 aatacaagtg ctgcaggagc gcacactcat agtgtcagcg gcacagctgc atctgcaggt
     2521 gctcacgcac atactgtcgg tattggtgct catacgcact ctgttgcgat tggctcacat
     2581 ggacacacca tcaccgttaa cgctgcgggt aacgcggaaa acactgtcaa aaacatcgca
     2641 tttaactaca ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2701 taaaaattta taatctgctg accggaacta atgaatttat tggtgaaggt gacgcatata
     2761 ttccgcctca tacaggtctg ccagcaaaca gtacctatat tgcaccgcca gatattccgg
     2821 ctggcttcgt ggctgttttc aacagtgatg agtcatcgtg gcatctcgtt gaagatcatc
     2881 ggggtaaaac ggtttatgac gtggcttccg gcgacgcgtt atttatttct gaactcggct
     2941 cattaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3001 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3061 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3121 atctggaaat cgcaacggag gaagaaacct cattgctgga agcctggaaa aagtatcggg
     3181 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct gtaatacctg
     3241 aagtttaaaa taaaagcccg cttaaaatat cgcgggcttc gaaatataaa tgtattttca
     3301 gaggctagag cttaggatat ctcagcggca attaacttat gagggatagc taaataccag
     3361 aagataatta gtttttacta tttatctttg tttgtggttc tccttcagca agctcagcgc
     3421 cagtgacagg attgatgtct tcatgggatt tcaaccttgt tgccatagct tttatcatag
     3481 cttcttgctt ggtcgaaaga tttatcgagg ccttcccatc cccaccgtca accgctgaaa
     3541 ctggatcgga aacatagtca aagttttcat cactgttcca actacctctg acgtcttcac
     3601 cttctgacat gttgtaatat acgtttttgt atttctcaag tggtggtaat tttcctggtg
     3661 gaaaggtgtt acgaattgaa tgtaatgctt tttcaaatga aagcatgtgt gctgcttccc
     3721 gggtcattaa aaatgccaga gtgtctttta ctccaggatc atcagtaaca ttaatgagac
     3781 gttcgtaaat gatctttgcc cgagcttcag ctgcaatatt tgaacgaaga tcggccgtga
     3841 cttcgccaat agtatcaaca taagctgcag tccagggtac tccagctgaa ttagttaatg
     3901 ctgggcctcc tccgtagagg agagaagtta tatggctatc attgccattt tgagttaagg
     3961 aacggtaaag ctcagcttca ttctcagttc cttcagcaag tgctcctttt gcacctttgt
     4021 tgagcatacc aacaagagaa ccaataattt cgagatgact tagttcttct gttgctatgt
     4081 ccatcagcat atcccttcgg cctgcatctt catcacttaa gccttgagtg aagtatcggc
     4141 atgctgctgc aagctcaccc tgtggcccac cgaattgttc taagagtaga ttagccaagc
     4201 ctgggtttgg ctcacttaca cgtactgtat attgaagttt tttcacgtgt ctaaacataa
     4261 tgcctcctca aaagaattat tttttggctt caactccatc aatttcagat ctgaccatga
     4321 attgctcagt ggtgtcagga atatggttaa gaagccattc agccatttgt ttttcttcat
     4381 taagaattgc ttcaataatg ggtactgaag ctgtatcacc cgcatttttg gctgctgcga
     4441 gaagagatgt gtaacacgca atttcaaatt gttcgaacac atagccactg atcgaacctt
     4501 tgacaatttc atcagatgga aaaattccac caatggattg tccaaacgcg gccattttac
     4561 tcatagagtc tttaatgaca gagcgcgaaa tattattacg atcaagtatg ctttcaagct
     4621 gtgaaagctg gttctttgtt tcgctaatgt gctgctcaat tctggaacgt aactcaggat
     4681 agttttctat ccggctggcc atagactcaa gcattttttc agcttgtttt tccatcgcat
     4741 gtgcatcacg aagccagtca tggtaatgtt caattctatt cataatctgt ttatcctaaa
     4801 agaaattgaa gtctatagtt caggagttat cctgagcttt tttattaacg ttattaaatg
     4861 ccaggtctgt taatttgaca tcagtagctt tttcctcttc cagtgtttct ttcaggagtt
     4921 tagccgcttt tttatatcct aattgctcag cgagtgttac caaagtgcca taactggcaa
     4981 tttcatagtg ctctactttt tgtgctgcag caatcaatgc agcatcacgt acttcatttt
     5041 tatcagtgct ttcaattacc tcattcgcct cttcgataag tccttccatt gcggcacact
     5101 taattctttt caatttaaga ccatcttcag aatcaacaac ctgatcgatg cgttcaattt
     5161 gaccatgtgt ctcatcaaga tgtgactgaa aagcagcggt taatttatca ctatatgcag
     5221 agcgactaag tttagacagt gcctttgtta gttgtttctc agcgctgtat gtatccgata
     5281 ataagtggat gaagatatct tcaacagatt taatttgcat aatcactcca gagttttcac
     5341 tgcgagcccg taggctcgca gttagcttca gttattcacg aatgtagggg gtgataaatt
     5401 aagagtcaga tttacggccg ccaccatggc tattctgacc accttttttc cctgcttctg
     5461 atgctctttg tggatcgttt ttaaaattac cgccactatg ttggccgcct ttacgaccag
     5521 cgtcagaagc tttttgtcga tcttcagcga aattacctgc accgccacga tgttcggtca
     5581 tatcatttct cctctgattt tcaaattgta aataacatgt gccttgtcag cacccaatta
     5641 actctagtaa caaataagaa agtgtcaaat aagagattgg tatgcgcatc ttaatgtgag
     5701 aaatagcaat tgtaaaaatg tgtactcagt gagtataatc tgttgatatc ttggtgatta
     5761 acggaaagtt taggttgtaa ctcaaaagat taataaatct aaattt
//
LOCUS       DAZFTN010000207         5751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFTN010000207 DAZFTN010000000
VERSION     DAZFTN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(246..854)
                     /locus_tag="ACHKV2_004884"
     CDS             complement(246..854)
                     /locus_tag="ACHKV2_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO6942607.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1280..1348)
                     /locus_tag="ACHKV2_004885"
                     /pseudo
     CDS             complement(1280..1348)
                     /locus_tag="ACHKV2_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1607..1954
                     /locus_tag="ACHKV2_004886"
     CDS             1607..1954
                     /locus_tag="ACHKV2_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942608.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2159..2860)
                     /locus_tag="ACHKV2_004887"
     CDS             complement(2159..2860)
                     /locus_tag="ACHKV2_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6942609.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2857..3876)
                     /locus_tag="ACHKV2_004888"
     CDS             complement(2857..3876)
                     /locus_tag="ACHKV2_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6942610.1"
                     /translation="MTGLKMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQNGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3873..4412)
                     /locus_tag="ACHKV2_004889"
     CDS             complement(3873..4412)
                     /locus_tag="ACHKV2_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO6942611.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4443..4665)
                     /locus_tag="ACHKV2_004890"
                     /pseudo
     CDS             complement(4443..4665)
                     /locus_tag="ACHKV2_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
     gene            4776..5468
                     /locus_tag="ACHKV2_004891"
     CDS             4776..5468
                     /locus_tag="ACHKV2_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6942612.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
BASE COUNT         1523 a         1353 c         1270 g         1605 t
ORIGIN      
        1 atgtaggaac tgaaaacaat attggatatc attttcggta tttctctgca aattccagcc
       61 cacaggatct cggtgtacaa tctgtcgctg atcgcgcaaa tttagggtca atcacttcat
      121 tttttgcatc aaaccctcac taacatcgaa cgattatagt acaagtagtg ttcttgactt
      181 ctcattaaac cgtcagcatt caattaatta caaaatacta gcggcatttc tctcccgcaa
      241 ctttattaca gccgttcaga acgtaaatgc gtcaatactc gactccactt atcatcctgc
      301 cacggccgga attttacatg tgccgtttct ctggcaagga ttgcctgtgc cttattgagt
      361 atccggggat attcttgttc gattgaagtg aagcgaccgg cttcgcggtg ttctgctacc
      421 tgaagaagcg gagtgacgtt ttggcaagcg gttaacattg tgtcacttgc ccgccataac
      481 caagcaagtg tgcaaagttc gttatcagtg aattgttttg tgattgggga ttgttgaatt
      541 tctcgatcaa gaatatccag cacccagcgg cggaattctt tggccacagg agtgcgggcg
      601 aacatggcga tcagatgggc gccacggagg gagaaaatgc gagtcttctt acgataattt
      661 cctgaggtcg ttgattcgac gacctgagac attccgtcag taaactcatc aatatttttg
      721 ttgtagatgt cggttactgc tttggtgctg gcatatttta atgctgccgc caattctttg
      781 ctggtaaacc agatctggtt gttgtgattt ataggagtga attttacatc gcggaatgcg
      841 agctgagtga ccatagcgat aatctcgttt tgaatttgag ctatcaccac cgacgacgcc
      901 aatcgactgg tggtggactg tgcaaggttg gcgtaaccgg tcaaaacgaa atccggcgct
      961 tccgaagaag ccctcacaca gcccaccata atttttgtgt agacgtgcac tgcgcataaa
     1021 aaaaccgcga acgcggttat gcgtcgtttt gatgtccggg acgccaatcc cgtgtgccga
     1081 ttttgcggca acgcacagaa tatagcgccg gataaataat gtcgtcaacc cttgcatatg
     1141 gggggcgcga tgatcactac taccaccata atcgctccaa ctgttgcaac tactttaggc
     1201 taagacatca cgatttccat atcaccttta gccgccagac aaacagagac tccctgttct
     1261 tggctgccaa aaacaccgaa tgcaaaaaat cgcaggtgta tttctgcatc tgtggaggga
     1321 gaagagagtt tggattgtat gtgcgcataa acgtccccgt ttagcgcaga agtcaccgta
     1381 gttgttcata ttctgatgac atgataattt cctattgaat agaaaaaatt aacacgactc
     1441 attcttatga ctggaaaaag caatcatgct aataatcatt gttgttccag ttccaaatgc
     1501 agcgaaataa tcaccaatat tttttctacc ggtttcgact ttgatgctat attctgctaa
     1561 cacctaacta acactacgtt tcacacagca attacatgga aataacatga ctatcaaagc
     1621 gactgacata gagattcttc atcgttatgc tgaaggggta atggaacgtt ctaaccatca
     1681 tgcaaaaaat gttggtgcag ccgctcttac attgctagga ggtgttatct ggaaagccct
     1741 gcccggatca atcgagataa gaacgtataa cggaagtctt gcaaatatgg tgtggtggca
     1801 aagcgaaaga acattaaaaa attatgcaat ctcatacaac cataattcat gtgaaattga
     1861 aatgagagat gaatcagtca aaggtgctgt attatttagc atttccaacg aaaccactcc
     1921 agaaaaaata ttatcacagc tttctgagct ttaattaaag gcggtgctaa caccgcccat
     1981 accattactc cacatcttac gcaacttgat aattatgctc tattaaatac ccacaatatt
     2041 tcatcaatgc atgggtcagc tacaaaatgt ttaccgtggt aattcctgtt ttcgcaacat
     2101 ctcaaaagaa caataaacac tgtatttctt caagtaacga agaatctttc tttgcccgtc
     2161 atacacttgc tcctttcagc ccaaacttag ctttgatttc tgcgatcttc gccagagcct
     2221 gtgcacgatt tagaggtcta ccgcccataa caggaagttg ttttactggt tcaggtatcg
     2281 tctcaccacg gttaattcgc gctgtcatac aggtcagttc atcggcagcc ttgcgccgta
     2341 attccgcgtc agtcagcgca ttggcccgca tgttctggta caggttggta accaaccagt
     2401 aatgcgcgtt cgatttccac ggataagact ctgcatccgg atacaggcca cgcttccggc
     2461 aatactcgta aaccatatca accagctcgc tgacgtttgg cagcccggcg ttaacggatg
     2521 cttcttcccg gcaccatgca acaaactgcc cgggtgatgg cagaaatggt ctattctgcc
     2581 gacgggcaac acgcattccg gcgctcacct gttccatcgt ggtgatcccg ttttcccgga
     2641 aagccagaac ccactggcgg cggatttcgt tcagttcgtt ctggtcacga ttagccaggc
     2701 tcgccgggaa agttgccagt aactggctga atacaccgtt gattatctgc gctacctgct
     2761 gtacctgcgg cttttcgtcg tactgttccg gcatgttgtt ggcgatccga cgcatctgct
     2821 cacggtcaaa gttaaccatc tgtgcggcga tgtttttcat aaatccaccc cgtaaatcca
     2881 gtcagtgttc gtcaggtcga gttttggttt gccggctgtc acgccagcct gttgcttgtt
     2941 tcggttgatt tcgagctggg tccacttgtc gcggagtttg gccggactca gcacgttacc
     3001 ggaccagaag ttgtcctggc aggcccagcg gaacagtaca cacatgtcgc ggtggttacg
     3061 tccatcacgt tcacgcatca gacggatatc gttagcccac cctgcaaaat tcggttttct
     3121 ggctgatggc gcgatggtct tcaccatgtc aaacatccac tctgcggcgg tcaggtcttc
     3181 tgctgtcccc cacttgctgc cgttctgaat cgcagcatcc gctttcacca caggaaggtc
     3241 gttttctggc aggtcagagg attcgccaga attctcggac gaataaggtt ttatattgtc
     3301 ttttgttagt ttgtcttttg tgtttacctg attcgggtaa gtgcctttac ctgatttggg
     3361 taaacttttc ttacctgatt caggtaaatt tacctctttc aggtaaactt tatttttctt
     3421 acctgattcg ggtaatgttg accattcact gaccacatta ttaatgccta tattccgccc
     3481 gctctgaata aaaatcccac gctttaccag aacacttttt gcagcagaac acttgtgcgg
     3541 caatatcccg gtcaactcgg aaagttgctc gttgctcacc caatccagtt ttttattaaa
     3601 gccatatgtt ttgcgcatga cagccaggaa gaccagaagc tggtgctgtg ttaatccggc
     3661 cagcattaca gcttccagca actcatttgc aatgcgcgta taaccatcat cgagatctgc
     3721 cacgcgcggc tccttttgtg ccgcatccgg cacaggaaaa ttgaatatct cagcagtgtt
     3781 tgccataatt cctcccgcaa tgagtgtgtt acgatttgca cctgaaagtc ggttctgttc
     3841 ccgcagaccg actttcgcca tttttaaacc tgtcatattg cccccaacat ggtggtaacc
     3901 atcgccatca atggaccagc cagatccggg tccacacgaa acatcgacac aataccttca
     3961 ctcatctcct tcagtttctg gtggcgtggt gcgttgagaa tgacagcctg ttttgcctca
     4021 ctgagttcct tttccatttc agccaaccta gccatgaagc tatcctgctc aaccaggtaa
     4081 ccgcgatatt ccagcggtag taccgccaga attgccgggg tcagttcacg cacgttattt
     4141 cggtattttt cagaatcgaa tttgttatcg aggaagcgga acagcttctg gcgtgtacgg
     4201 ctgacatcat cagggaaatc gatggtgccg ccgccctgct cccgatactc attcacaatg
     4261 agtgtggcaa cgacatcctg attatctaca gccgaccagg cgcggacggc atcacggatt
     4321 ttttcgtggc ctggcacctg ttttatttga gaacgattta tcaccgcagt cgggctaaat
     4381 ccgctagtct gttggtatgt aagtggttgc ataatcattg ccttatcagt taacgccgca
     4441 gtttaggcgg cagaattact cgcgttaaac aatggtgcaa ggtcgggacg aatatctgct
     4501 ggtttaatct ttccaccagt ggctgagaca attttcatta catagcgggc atcaattccg
     4561 ccaccgtgta gccaacgcca aacagtgggc tacaccgcat agatctgcca gtcgtttttg
     4621 actacctgta atactgattg cgagttgaat ggtttgattt gtcattatca attcctattg
     4681 gtattacaat gaatggataa tagcaatgcg tattaatcca atcaatagca aaacgtgttt
     4741 tgaccatcaa tacgcaagcg tataaattaa aacttatgaa aaaagaaact cttgctgatc
     4801 gcttaaacct agcgatggaa caatctggaa tgtctcaagg cgctcttgca aaggcgtctg
     4861 gcgtagctca acccacaatc tggagactga caagcggcaa cgcacgcggc tcaacaaaaa
     4921 ttgttgaaat agctaatgca ttgggtgttc gaacagagtg gctctcatca ggcataggcc
     4981 cgatgagaaa tgacggtcaa caatcaggga agcctgctgt caaccattcc aaatacttca
     5041 agattgacgt tcttgatata gaagtcagtg ctgggccggg tgtcatcaac cgtgagtttg
     5101 tagaagtcct acgctcggtt gagtactcgt ttgacgatgc tcgtcacatg ttcgatggca
     5161 ggaaggcaga aaatatccgc atcattaacg tacgcggtga cagcatgtca ggaacgatcg
     5221 aaccaggtga tcttctgttc gttgatatca ctgttaaatc tttcgacggt gatggtatct
     5281 atgcgtttct gtacgacgac acagcccatg taaagcgcct gcaaatgatg aaggataagc
     5341 tgctggttat ctctgataac aagagctact caccgtggga cccgatcgaa aaagacgaaa
     5401 tgaatcgggt atttatattt gggaaagtta ttgggagcat gccacagaca tatagaaaac
     5461 atgggtagat tattaatgac agatttcatc aagataacca gatgatgttt tggtgagaca
     5521 tgctgacatc ctcataattt atagaatgga tgtttcaagc agagcttaag gtatatggat
     5581 atgtcaaaaa aaaattttga attcaaaaat tacccaatag tctttattgg ctcaggcatt
     5641 tcaaaaagat atttagagaa ctacccaacg tgggaagagc tattaaacga gtattggaaa
     5701 ataacaaatc ccacaaatga tttctatagt tatcttttaa caataaaaga a
//
LOCUS       DAZFTN010000208         5703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFTN010000208 DAZFTN010000000
VERSION     DAZFTN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..570)
                     /locus_tag="ACHKV2_004892"
                     /pseudo
     CDS             complement(37..570)
                     /locus_tag="ACHKV2_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(575..739)
                     /locus_tag="ACHKV2_004893"
     CDS             complement(575..739)
                     /locus_tag="ACHKV2_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942613.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(736..1416)
                     /locus_tag="ACHKV2_004894"
     CDS             complement(736..1416)
                     /locus_tag="ACHKV2_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6942614.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1504..1965)
                     /locus_tag="ACHKV2_004895"
     CDS             complement(1504..1965)
                     /locus_tag="ACHKV2_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6942615.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1958..2998)
                     /locus_tag="ACHKV2_004896"
     CDS             complement(1958..2998)
                     /locus_tag="ACHKV2_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO6942616.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2970..3521)
                     /locus_tag="ACHKV2_004897"
     CDS             complement(2970..3521)
                     /locus_tag="ACHKV2_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6942617.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3505..3732)
                     /locus_tag="ACHKV2_004898"
     CDS             complement(3505..3732)
                     /locus_tag="ACHKV2_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6942618.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3809..4216
                     /locus_tag="ACHKV2_004899"
     CDS             3809..4216
                     /locus_tag="ACHKV2_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942619.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4480..4779
                     /locus_tag="ACHKV2_004900"
     CDS             4480..4779
                     /locus_tag="ACHKV2_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942620.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            4851..5069
                     /gene="ydfC"
                     /locus_tag="ACHKV2_004901"
     CDS             4851..5069
                     /gene="ydfC"
                     /locus_tag="ACHKV2_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6942621.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5638..>5703
                     /gene="dicB"
                     /locus_tag="ACHKV2_004902"
     CDS             5638..>5703
                     /gene="dicB"
                     /locus_tag="ACHKV2_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6942622.1"
                     /translation="METLLPNVNTSEGCFEIGVTIS"
BASE COUNT         1386 a         1511 c         1281 g         1525 t
ORIGIN      
        1 taaggaaaat tatacgcata ttgaagagta taaaccttac atgttgatta catttttgta
       61 atcaacatcc tgtttggaat agccagcctt taatggataa ctatttctga caatgcaatg
      121 agtataatca agtccatctt ccactgagaa ttagaggcgg catgcttttt ccggctcttg
      181 ccggatatcc gtaattgtcc ataatctgca gatttatacc ttctgcatga cctgtcagcg
      241 aaaatttgtc cggtgtttct acgggaatga catcaaaagt tacacgcact cgcgttaccg
      301 tgtagaccta ctttcctgca cttgcaagat cacagtggtg taaccgtaac aggaatttat
      361 tctctggacc ggcagtaaat ccctgagtgg cgtggttccc atatcaattt cccgccaggc
      421 agcctccatt gccagcgtac aggctggagc catgacctgc cctttaaatc tggcccgacc
      481 atcccaccgg acgtgttctt ctcccctgaa cttaggtaca gtcatctcca gtggcacaaa
      541 agtgtcagcg ccataatttt tgaccgttat cgcgctacgg atattttgtt gactggtgaa
      601 aatcaccccg cagaatcagg cttattccct taacctgggc tttcatcctg accgccgcct
      661 cactacgacc aatcagactg ccgatgcatt ttaccttcat tgttagtatc atgatttcag
      721 gcctgcacca tccgctcatt gcccggactt ccgacaaatc ccggcaacca tatcccggtg
      781 cttgttcagc tcccgcagcg cggcgcagac tcgctcccac ttctggacat gattcttcgc
      841 cctacgcagt tcgcgatttg ccatatgcag cgatggcaaa atcaaatcat cttctcgcgt
      901 tgcagtaaac gatggcagcg actgcacaat gtccgccaca gtttctgttt taatatcttc
      961 ctgtgttgca tctttctgta ccggtaacgc aacaccggct ggctgaggaa aggctttacc
     1021 atcggtttcc gctaccgatt caactttcgg ctctgctggt aaattatcac ctggtatgca
     1081 gtaacgaatt ttaccgttct ggtttacgcg aatcagacga cctttgctga ttgccattgc
     1141 cagcgttgaa gcaactttgc gggatgttgt accgaacagc gtagccagtt catccgccgt
     1201 ttgtggtccg cgttgttcaa tcgtcgcggt taaatcgcac tctgagattt tcgcttctgt
     1261 tgccgtggtg gtttcttctg gcgctggctg ttcctgctga acgttgttat cagccacacg
     1321 ccaggtgtat acgcttttat caacgaagcc agcctttttc agttcccaca gctcgttcag
     1381 tacttcttca cgactgatat caagtcgcgc agccagctat accgacgtgg cttttcccat
     1441 cgctttcagt gcgtcaaaaa cagtctccat aaatttcctc ccggtaaaaa atcacttctc
     1501 aactcaaaca aaaccagccg ctttccggcg ttcatattcc tgtttcagca actcaattgg
     1561 cgttggcccc gacgggcgtt tgggggccgc cagttgtcgc cggactggcg gaacgctcag
     1621 gccgttacta acatgctttg cccatttcgt cagttgccgt tctgcaagcc gttttaattc
     1681 cccttcggtc atctggcgct caatcccctt tgaacgcatc tcgaggcaaa tgtgatacag
     1741 cacaggctga gaccacgggt acttatcact tccgtcgtat cgccaggact cattgcgcca
     1801 gcggcggtac tcctccatca cagcatccac cgtcagaccg aatggattgg ctccgctttt
     1861 cgaaatcagt gccacaaact cagccaggtc cggaggccat gtttcacccg cccggcagcg
     1921 gtccatgcac tggcggcaga cctgccggat ttgctgctca gtcatcgcgc caatctgtgc
     1981 aatccagagc ttcgaaggtg cggccccgtt cttctggatc cagcggttcg aataaaccac
     2041 ccccatgagt tcccacagct tccaggccgt ttccgtcgct gataaatccg ttttcacgtt
     2101 cccactgctc acgtgctgcc cgaatttcct gaactgcccg tgatgcggtg ccacctggtg
     2161 ctgctgcatg gtttacccct ttgctgactg gtttaacctg cgccctgacg tgatttacgt
     2221 gacgggcgaa tttctgctcc cactgaatct gcgtaaacac tttcccctcc gctgcccagt
     2281 agtcccggaa ggcggcaagt tcagcaggtg taaattctgt ctccggcaaa gccatccccc
     2341 acaacgcagc ccgtcgtcga aaatcccgtg acggatacca gctatcgctc atcgggaatt
     2401 ttccgatggg ttcgctcagg ccatacagga atacaggggg ggctgcctgt aacgacaaaa
     2461 cttcctgctc actggtcgga gcactctcgc gtgcgttatg tgtggggttt agatctttgg
     2521 gttcctttgg gttccgtgat ccgtttttgg gtgtctttga tggaaaattt gggtgtcttt
     2581 ggttattttc catgcagcta agagttctgt ttttgggtct gttttgtgct gaaacataac
     2641 cattttcggt actgttttta ttaacagcac caattttacc cacctttaaa gactcccgtt
     2701 tttgggtgta ttcaggctcg gcaacacttt cttctacacc gataagtcgg tacaccacaa
     2761 tttgctttgt tctgcctttt ctctcaccgg tatcaacaat taacccaatc tccatcaggt
     2821 gtcgtaagct gtcctgcaca gtctttttgt tcagttccgt tacttctgcc agtgcagata
     2881 cagacgggta tgcacacaaa tcggcaccgc acatatcagc aagccaggtc aatactgact
     2941 tactggatga actgccggtt ttcacctttt tagcccatcg tagtgcatcg atactcatac
     3001 gcccccctgg cagacatttg tttatctgca aagtaatatt ggtattgctg acgatacgcg
     3061 tgcttgaaag caatagcttt ttctataagc tcgtcagtct cacgttccac aacaactgga
     3121 tccgcaaaaa gcagcccgga ctccaccaca tcgccatatt ctttgtttaa cccggcgatc
     3181 atgtacgtaa tgctttttcc gtcagtaatt tcacaataca atctgaaatc gctgatccgg
     3241 atagcctcca taattgccgg aatcagcgcc gtgaatttgt cacgcttatc tctggtgtcg
     3301 atagctttcc agcgttcgaa tatcttcacc cggttaacgc ccagcgcccg ttgatcaacc
     3361 tcgccatcat taaacgtgac gcgttgaaca tcgatgttcg ggcgttcttt cagagcccag
     3421 aatgcttccg tgattaatat cgtcgcctgc tcctgtgtca ttcctggtcg gcatacccag
     3481 gtatccagag cctcacaaac ctgttcagtg gtgattttca ttgttcaacc gccccgcccg
     3541 ctttgcctta cgatattcgt cataaacttt ggggtcgtac tgaagttccc cgccggatgc
     3601 ctcttgcagg cgcatcgcgc gaccttcagg aaccagttcc ccccatgcag caacgcttgc
     3661 cagcctaact cctgcgacat tggcaagctt tgttttgctg ccaaaaaacg ctatagcatc
     3721 aattttcaac atatcgaaac ccttagattt tcctaaggaa actagatcgt agagaaacct
     3781 aagtcaagaa aaattagaat tacctaatat gaaaaacgaa accttcggtg ctcgcctctt
     3841 atacaggcga aaaaaattaa aactgtctca ggccgcatta ggtaagctgg tcaaagtggc
     3901 tcacgtaaca atttctcaat gggaaagaga tgaaacacag ccagcgggga agagattatt
     3961 cgcactgagc caggcgcttc agtgctcgcc gacttggctt ctttttggag atgaagataa
     4021 gcaaccaggc gaaccgatcc cagataatca gccagtcaat ctgacagaag atcaaaaaga
     4081 gttgcttcaa ctgttcgacg cactgcctga gtcagagcaa aaggctctgt tgtcagagat
     4141 gcgtgctcga gttgagaatt tcaacaaact ttttgaagaa ctactcaaag ctcgcaaaag
     4201 aagcgcaaat aaataacccc cctttttttc gcaactctct gtaataaaaa gcacaaactt
     4261 tcaaatactt gtgtttttta catcaagaag cttaggtttt tctacacaaa aagcttgacc
     4321 ataactctta ggctattcta aattccactc atcaagacac cgcacggtgt tctcagcaaa
     4381 cagttccgct acccggcgtt aaggggaagc agaggatttc tcagtgggcg agtcaaacat
     4441 cagaatggaa ggcgtccagg gatcagcaaa gaaacagcga tggcgcttta tattgatatc
     4501 agcgccattg ccggacaggt aagagttatc agagcggtaa ctaagcggta tgcgccttta
     4561 cttcagaaag tctctggtga gtgcaccgaa gatattgtca acgatttcgt catcgaactg
     4621 cgaggactca tcttcagtta caaggtgacc acaatttttg cagatggctc ccgcgaaact
     4681 gtcagagccc tgcggcttaa aggatgtgtc aaagacttcg ccaccacatt ctgggcaaga
     4741 aaacttgatt gtattcataa ccaatttcct ctcgagtaac agacccctca gaggatacca
     4801 cctcgcctga cgtggttaaa agcaggcaac gctaaccaca aggagccgac atgcagaaac
     4861 gagaacccgt catcatcgcg ccagactata ccgatgatga actttatgag tggatgcacc
     4921 agaaaattaa tgcagcgcag gatctgaaat gggccaatga agccagggct aagcaggctg
     4981 aaaatctgtc cgctctggag caggatatca ccaatctgga aaaagcagcg gcattaagca
     5041 ttgccagaat gattacatac ccgcgttaat agctaaccaa cgaagctaag gttggtaatt
     5101 aaggagttct ccacgggtga ggtggagtgc gtgcgccgga cacgggtgag catacggcac
     5161 tgacagttta ctgaaaggat atttccctga aaagtcagac cataacgcga aagcgcacgg
     5221 cgaggtagct ggttcataga tagcctgtcg ttaaattttc gtcgaccgtg cgcttccggt
     5281 tgtggcaatc cgcgaaatgg cgcggcggta agtatggagg ggttattcct tcccccgttg
     5341 aggacaccgg gttgtcaggt tgaccatacg cttaagtgac aaccccgctg caacgccctc
     5401 tgttatcaat tttctggtga cgtttggcgg tatcagtttt actccgtgac tgctctgccg
     5461 ccctttttaa agtgaatttt gtgatgcggt gaatgcggct gagcgcacgc ggaacagtta
     5521 aaaccaaaaa cagtgttatg gggggattct ctgtatccgg cgttaattgt taactggtta
     5581 acgtcacctg gaggcaccag gcaccgcatc acaaaattca ttgttgagga cgcgataatg
     5641 gaaacgttat taccaaacgt taatacgtct gaaggttgtt ttgaaattgg tgtcactatc
     5701 agt
//
LOCUS       DAZFTN010000209         5438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFTN010000209 DAZFTN010000000
VERSION     DAZFTN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..379)
                     /locus_tag="ACHKV2_004903"
     CDS             complement(<1..379)
                     /locus_tag="ACHKV2_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942623.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="ACHKV2_004904"
     CDS             complement(436..831)
                     /locus_tag="ACHKV2_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6942624.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="ACHKV2_004905"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="ACHKV2_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="ACHKV2_004906"
     CDS             complement(1642..2382)
                     /locus_tag="ACHKV2_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6942625.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="ACHKV2_004907"
     CDS             complement(2389..3351)
                     /locus_tag="ACHKV2_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942626.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVAPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="ACHKV2_004908"
     CDS             complement(3374..3799)
                     /locus_tag="ACHKV2_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6942627.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="ACHKV2_004909"
     CDS             complement(3796..4098)
                     /locus_tag="ACHKV2_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6942628.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="ACHKV2_004910"
     CDS             4196..4567
                     /locus_tag="ACHKV2_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942629.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="ACHKV2_004911"
     CDS             4588..4779
                     /locus_tag="ACHKV2_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO6942630.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="ACHKV2_004912"
     CDS             4781..5059
                     /locus_tag="ACHKV2_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6942631.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT         1313 a         1498 c         1217 g         1410 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacggggcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gtttagtttt tacttgactt aagtttaatg
     5161 tttattaaac taaaaatacc accccgcccc acagaacgca gggaaatact tcgagttacc
     5221 cggcagtggt caggggttaa gtagccagcc cgaggcgtat gaacatgacg gcagggttca
     5281 actttaataa ctatgcagca gttttttgtt ccgctacccc ggcgttaagg ggaaacagag
     5341 ggtttctcag tgggcgaagt caaacatcag aatggaaggc atcccgggat cggcaaagaa
     5401 gcagcaatgg cgctttatat tgacatcagc gccattgc
//
LOCUS       DAZFTN010000210         5430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFTN010000210 DAZFTN010000000
VERSION     DAZFTN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..384
                     /locus_tag="ACHKV2_004913"
     CDS             <1..384
                     /locus_tag="ACHKV2_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6942632.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHKV2_004914"
     CDS             419..1117
                     /locus_tag="ACHKV2_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6942633.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHKV2_004915"
     CDS             1139..1363
                     /locus_tag="ACHKV2_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942634.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHKV2_004916"
     CDS             1360..1716
                     /locus_tag="ACHKV2_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFO6942635.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHKV2_004917"
     CDS             1749..1901
                     /locus_tag="ACHKV2_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942636.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHKV2_004918"
     CDS             1898..2209
                     /locus_tag="ACHKV2_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942637.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ACHKV2_004919"
     CDS             2336..2899
                     /locus_tag="ACHKV2_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942638.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ACHKV2_004920"
     CDS             3009..3113
                     /locus_tag="ACHKV2_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942639.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ACHKV2_004921"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ACHKV2_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6942640.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ACHKV2_004922"
     CDS             3680..3958
                     /locus_tag="ACHKV2_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942641.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="ACHKV2_004923"
     CDS             3960..5009
                     /locus_tag="ACHKV2_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6942642.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            5022..5381
                     /locus_tag="ACHKV2_004924"
     CDS             5022..5381
                     /locus_tag="ACHKV2_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6942643.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVPGERLGIKITELECA"
BASE COUNT         1444 a         1226 c         1521 g         1239 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 taccttaccc gccgacggtg aacacctact ggcgacgtcg tggcagcaca tattttgtat
     5101 caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag cagcggctga
     5161 aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg gataagcgcc
     5221 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
     5281 taatggacga tgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg
     5341 agcggctggg gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt
     5401 tgtgcgtgag ctgctcatta tcgctaccgc
//
LOCUS       DAZFTN010000211         5328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFTN010000211 DAZFTN010000000
VERSION     DAZFTN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..283
                     /locus_tag="ACHKV2_004925"
     CDS             <1..283
                     /locus_tag="ACHKV2_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO6942644.1"
                     /translation="IHIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREIT
                     FQQYRENLAKAGVFRWVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            575..868
                     /gene="bor"
                     /locus_tag="ACHKV2_004926"
     CDS             575..868
                     /gene="bor"
                     /locus_tag="ACHKV2_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO6942645.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(959..1141)
                     /gene="rzoD"
                     /locus_tag="ACHKV2_004927"
     CDS             complement(959..1141)
                     /gene="rzoD"
                     /locus_tag="ACHKV2_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO6942646.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1358..1834)
                     /locus_tag="ACHKV2_004928"
     CDS             complement(1358..1834)
                     /locus_tag="ACHKV2_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO6942647.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(1821..2126)
                     /locus_tag="ACHKV2_004929"
     CDS             complement(1821..2126)
                     /locus_tag="ACHKV2_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6942648.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(2448..3137)
                     /locus_tag="ACHKV2_004930"
     CDS             complement(2448..3137)
                     /locus_tag="ACHKV2_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6942649.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(3134..3274)
                     /locus_tag="ACHKV2_004931"
     CDS             complement(3134..3274)
                     /locus_tag="ACHKV2_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6942650.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(3271..3633)
                     /gene="rusA"
                     /locus_tag="ACHKV2_004932"
     CDS             complement(3271..3633)
                     /gene="rusA"
                     /locus_tag="ACHKV2_004932"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6942651.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(3630..3920)
                     /gene="ybcO"
                     /locus_tag="ACHKV2_004933"
     CDS             complement(3630..3920)
                     /gene="ybcO"
                     /locus_tag="ACHKV2_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6942652.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3913..4083)
                     /gene="ninE"
                     /locus_tag="ACHKV2_004934"
     CDS             complement(3913..4083)
                     /gene="ninE"
                     /locus_tag="ACHKV2_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6942653.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(4083..4538)
                     /gene="ybcN"
                     /locus_tag="ACHKV2_004935"
     CDS             complement(4083..4538)
                     /gene="ybcN"
                     /locus_tag="ACHKV2_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFO6942654.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(4535..4633)
                     /locus_tag="ACHKV2_004936"
     CDS             complement(4535..4633)
                     /locus_tag="ACHKV2_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942655.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            complement(5040..>5328)
                     /locus_tag="ACHKV2_004937"
     CDS             complement(5040..>5328)
                     /locus_tag="ACHKV2_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6942656.1"
                     /translation="ELRRALVSLKTLKSKGVNSFSDFYAIDLTSKNGRELCRTLAYKI
                     FEKIIINTDNKTCDIYFMNGIVFKHYPLMKVISAQQAISALKYMVDGEVYF"
BASE COUNT         1390 a         1290 c         1186 g         1462 t
ORIGIN      
        1 tattcacatt gtgttagaaa acgataacac tgtgttaata aaaggactta aaaaggttgt
       61 gaatgttaaa ttctcaagaa atacgcatct tatagaaacg tcctatgata ggttgaaatc
      121 aagagaaatc acatttcagc aatacaggga aaatcttgct aaagcaggag ttttccgatg
      181 ggttacaaat atccacgaac ataaaagata ttactatgcc tttgataatt cattactatt
      241 tactgagagc attcagaaca ctacacaaat ctttccacgc taaatcatca cgtccggttt
      301 cttccgtgtc agcaccgggg cgttggcata atgcaatacg tgtacgcgct aaaccctgtg
      361 tgcatcgtta ttaattattc ccggacactc ccgcagagga gtttccctgt cagggctgcg
      421 gacatagtta atccgggaat acagtgacga tccttcgcat ctgacataca ttaataaata
      481 ttaacaatat gagatttcaa ctcattgttt agggtttgtt taattttcta cacatacgat
      541 tctgcgaact ttaaaaagca tcgggaataa caccatgaaa aaaatgctac tcgctactgc
      601 gctggccctg cttattacag gatgtgccca acagacgttt actgttcaaa acaaaccggc
      661 agcagtaaca ccaaaggaaa ccatcaccca ccatttcttc gtctctggaa ttgggcagaa
      721 gaaaactgtc gatgcagcta aaatttgtgg cggcgcagaa aatgttgtta aaacagaaac
      781 ccagcaaaca ttcgtaaatg gattgctcgg ttttattact ttaggcattt atactccgct
      841 ggaagcgcgt gtgtattgct cacaataatt gcatgagctg cccatcgata tgggcagctc
      901 tatctgcact gctcattaat atacttctgg gttccttcca gttgtttttg catagtgatc
      961 agcctctctc tgagggtgaa ataatcccgt tcagcggtgt ctgccagtcg gggggaggct
     1021 gcattatcca cgccggaggc ggtggtggct tcacgcactg actgacagac tgctttgatg
     1081 tgcaaccgac gacgaccagc ggcaacatca tcatgcagag catcattttc agctttcgca
     1141 tcagctaact ccttcgtgta ttttgcatcg agcgcagcaa catcacgctg acgcatctgc
     1201 atgtcagtaa ttgccacgtt cgccagcttc agttctctgg catttttgtc gcgctgggct
     1261 ttgtaggtaa tggcgttatc acggtaatga ttaacagccc atgacaggca gacgatgatg
     1321 cagatgagca gagcggagat aatcgcggtt attctgctca tacctcactc tctctgaccg
     1381 ttccgccagc ttctttgaat tttgcaatca ggctgtcagc cttatgctcg aactgaccat
     1441 aaccagcgcc cggcagtgaa gcccagatat tactgcaacg gtcgattgcc tgacggatat
     1501 caccgcgatc aatcataggt aaagcaccac gctctttaat ctgctgcaat gccactgcgt
     1561 cctggctttt gggggagaag tctttcaaac caagctgttt acggtaagca tcccaccagc
     1621 gtgaaagaag ctgataacgt ccggcggctg ttgatttgag tttggggtgt agcgtgacaa
     1681 gtttgcgagg gtgatcggag taatcagtaa acagttcgcc gccaacaata acatcataac
     1741 cgtggttacg tgtcggttgt cgcccgttat ccgttccttc tgaccacgcc aacatatcga
     1801 ggaaggcttt acgctgagga ttaagatttt gcatttttca cccctgtcag tcgttcccag
     1861 aagtacgtca gtgcaaccga acccatcgca ccactaatcc ccgctgtcgc gagaatcatg
     1921 taaatactga atccactttc gatgctgatc aggccaccaa taacaccggt gaatcctgat
     1981 accactattt gagccagagc atttatccaa ctccacgttg ctttactctg cttcacatct
     2041 atcaggtagc ggaccagacc gccccaacct gcgatgatca gcaaaacgag ccagaacgct
     2101 ccggcaaggc tctctttttc gtgcatatga atagccaatg tttcgccgcc gacaaaaggc
     2161 cgggacgtta aatgtcagaa atcaggctca cggggtaatt taacgacaaa gcacggagtt
     2221 gatgctcccc acaagcctgg aataaaaaag ccagcatgta gctggcaaca gagggctaag
     2281 caatatcaac tcaacagctg aagaccccct ggctggggta ggttggaagg ctactcaccg
     2341 ttcagaaaca gaaaagccca aggctttaaa cctcgggctt gaatttggat tactgccagt
     2401 gcgtacaaca ttggcaaaat atcagattta cataaaatat atgattttta atccagtttt
     2461 gcaatatctt gctgtgaaaa tgtggtcttt tgttttgaac gtgttttcgt taaaagcaat
     2521 aaagcttggc tatcaagctg tagaaaaatg tgtttcattg caacccagcg ttcagtaaat
     2581 gtctcagacc agttttttga tgtcactccc accagtgatg ccagctcctg gtattcatag
     2641 gtcttacgcc ctgccagctc gttcttcaca tcctgtgccg ccagccagat caacttcttc
     2701 aaacgttcca gtgtcttacc tgcaattttc ctggtaccca acagagtctt aaactcgctc
     2761 catgcccact gcgttatggt gacctgatgt tcccagcgaa cactttcgct gtaactccac
     2821 agcaaccacg ctttctgatg ttcttcgaga gacagaaccg cgcggcgcca tgacgaggtt
     2881 gagaactcaa cctggctgac cagtgcaatg gatgaacctt ttgcgtacga ctgcttaccg
     2941 gaaatcggcg gattatccag cgtaatcatc ctgccagtta cctcatccag aatgcgcggc
     3001 ttctttcgtt tgtatgtacc agtatcaaat tgtgcatgct ccagccaggc ttcaagctgt
     3061 cctttcgttg ctccgctcaa atcagcggta gccacaatga gttgctcgcg gacatactgt
     3121 aaatattggg tattcatgcg gcagctcctt tcagtgtttt ggcgtaattc ttcagtatcc
     3181 ggtaatcggt caaaacagaa ccaggaaaac gatataagcg caggcgcatc cagcggtggc
     3241 gaagacgttc tgccatataa gactcaaaca tcattcattc cccagttcgg tgatggtcag
     3301 ctccagcttc ccacctttgg taacgggcat cttcacaaca cggtaatcaa cgacctgagc
     3361 atcatccagc cagaaacctg ctttggtgag tgcgtcaaaa gcggcttttt gcagattatc
     3421 caggtcacgg cgacggcgat ccggcatgtg gcactcaata cggattttca ctggcatagc
     3481 caggccgata tccagcatgg agcctttgat gattcgggcg acgttatcac ggtatgcctg
     3541 cccttctgcg ctgatgtgcg tgcgcccgcg attatgccgg tagtagcgat tattgctcgg
     3601 aggccagggt aatgtgatgc tgtaggtatt cacgccttaa taaccccctc tttcagccag
     3661 ataacctgtg ttctcgccat accttccagc gcgcattctt ttgcatatgc agcatcgaca
     3721 aaatgtgtgc ggcggtcgat ttcgtcgtgg caggcagaac atgcaatggt ggcaatcaag
     3781 tctggcggtt tggtaccggt gccgcacaat ccagtcagcc ggatatgtgc cagtacagac
     3841 gtttcagggt tgccattaca tacgccaggg attcttactt ggcattcccg actacgcgct
     3901 gcttttctca aatcagccat gattcctcct tgctgccagt cgcaaccatt ttttatcaac
     3961 caggctggcg gtatatccga gcagtgttgg tatttcggat ggcttcagct caggtttacg
     4021 cttacgacga tttggtactt tgtagatgtg tccgttcatg acacgaataa gcggtgtagc
     4081 cattacgcct cctgcttgtc acgcagcagc tggaactcgc agctctgtgg aatagtcagg
     4141 tggcagccaa tattcatcgc ccaggcttca accttacaca ggaagacata catctctccg
     4201 gtaccaagat cggaggtatg gcgtaacgac tggatagtag tgatttcgcc ggttacgaca
     4261 tcaaccaggt ccttggtttc ataaccgagg tatgtgtgtt tgagagcatc ttttacccat
     4321 gctgaagtag cgaacgattt ccccctgctg atgaggtatt cactgatttc gctgtaccac
     4381 atgtggctga gtgcattctg ggaaagactg cgtctctcgc gccacggttt aagcaccatg
     4441 cgaaagcatt tgccgtcttc cagataagtc tggatctgct gaccgacagc ggtgaagtta
     4501 ccgcgatgta atttgatgcc atcttgtggg aggttcacgc ttcacctccg cagagatcaa
     4561 acgctggatg caagacatcg caggtgcatt tctgcatctg tgacaaggta aggagttcag
     4621 attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg ttcaagctcc
     4681 ggtgacatta ttatggaagg ttgatactaa aaaatcaaaa attgaatatc attggcgata
     4741 gtctagcttg tgatatccac aagctacttc aatgaagcga cgagtaacat tttgtgtcat
     4801 ccatgttttt tcagttaagt gccacaccca gttcaaaatt tgctgatgtg ttgaacatgt
     4861 actaagcggg atgttgtatt cataagcaac attgattccg caaaatcggt gtcgtgtcat
     4921 gaccgattca cgttgacctg cgccctgttg agtgcctggg gatattcttg ctcgatagtt
     4981 aatatcatag tagttacttt actttcatac catagcacgg ttgaaaaagt gattattact
     5041 caaaaataaa cctcaccatc aaccatatat ttgagagcac ttatcgcctg ctgggcggat
     5101 attactttca ttaaaggata gtgtttaaaa acaatgccat tcataaaata gatatcacag
     5161 gttttattat ccgtattaat tatgattttt tcgaatattt tataggcaag tgtacggcat
     5221 aactctcgtc catttttact ggttaagtca atagcataaa aatcactgaa tgaatttaca
     5281 cctttactct tcaaagtttt caatgatacc aaagctcttc gtaattcc
//
LOCUS       DAZFTN010000212         5287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFTN010000212 DAZFTN010000000
VERSION     DAZFTN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            287..445
                     /locus_tag="ACHKV2_004938"
                     /pseudo
     CDS             287..445
                     /locus_tag="ACHKV2_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            669..788
                     /locus_tag="ACHKV2_004939"
     CDS             669..788
                     /locus_tag="ACHKV2_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO6942657.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            853..4689
                     /gene="ypjA"
                     /locus_tag="ACHKV2_004940"
                     /pseudo
     CDS             853..4689
                     /gene="ypjA"
                     /locus_tag="ACHKV2_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
     gene            4690..4794
                     /locus_tag="ACHKV2_004941"
                     /pseudo
     CDS             4690..4794
                     /locus_tag="ACHKV2_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4894..5100
                     /locus_tag="ACHKV2_004942"
     CDS             4894..5100
                     /locus_tag="ACHKV2_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942658.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
BASE COUNT         1547 a         1148 c         1327 g         1265 t
ORIGIN      
        1 tgattgagta aacgggatta ttcttcacac tacatccatt ttagtgtcac taccaaagaa
       61 aaaggggcta cgttttcatg taaccccttg atttatttgg tgggctggca gtgactgaat
      121 tggtgctgta attggttgaa atatttcatc tattgtctgg ttcaactttc cttagctgcc
      181 tgaaagtttt ttgatctcac ccagcaataa cagcgttacg gtgcaccacg cctgactgaa
      241 gaactgcgtg ctcagggtta ccgcttcaac gtaaaaccgt ggcaggaggt ccggactgaa
      301 aaaactgctc aggacatcat cggcagggaa agcactgcta gtctgtaaac tgaaccgact
      361 ggggcgcagt atgtggcatc ttgtcgtgct gttggaggag ctgtgcgaac gtggtattaa
      421 ctttcgtgct ctggcccaat ctatatttgc ccaacaatgg ggggacgaat gctgtaaaag
      481 taaaacaatc tgcgatctca aagttattgt gtgattttta tgtgagcaga agatattcat
      541 cagaaacgat tatgtaaatc attttatttt gccaacggcc tcattgtcga aagaaaagaa
      601 tacgcccgta tttcctgaat ggattgactt ttttatccaa ccacacttca gcgcactgcg
      661 tttaaaaaat gcctcattct tatgcggaat atcatcattt catcatgatg tctttgatga
      721 gcggtgaaca caatacactt gcgctgtctc ttcaggatga atcccctggt ctggtgcctg
      781 tgggctgatg ttgcagcaaa gctaaggtcg cttaaacgct actcagtatt cacttttcag
      841 aggatgaaat ttatgaacag gaccagtccc tattattgtc gtcgctcagt actttcctta
      901 ttgatatctg ccttgatata tgccccgccc gggatggctg ccttcactcc tgatgttatt
      961 ggtgtggtaa acgatgagac tgtagatggc agccaacgag tagatgaacg aggtacaaca
     1021 aataacactc atattatcaa ccatggccag cagaatgttt atggcggggt atctaatgga
     1081 agtcttattg aatctggtgg atatcaagat gtaggaaggc ataacaatta tgtggggcag
     1141 tctaataata ccaccattaa cgggggcaga cagtcaattc atgacggggg tatttccaca
     1201 ggtacgataa tcgagagtgg caatcaggac gtttataaag ggggtatcag caatggaacg
     1261 acaattaagg gcggtgcttc acgcgtagag ggagggagtg cgaatggaac actcattgat
     1321 ggtggtagcc agatagtaaa agttcaaggg catgctgatg gtacaacgat aaataagtct
     1381 ggctctcagg acgtagtaca aggaagtctg gcaacgaaca caaccataaa tggtggtcga
     1441 cagtatgttg aacagagcac agtagaaaca accaccatca aaaatggcgg tgagcaaaga
     1501 gtatatgaga gccgtgcgct ggacacgacg attgaaggcg gaactcagtc tctgaatagt
     1561 aagtcaacgg caaaaaatac tcagatctat tctggtggta cgcaaattat tgataacacc
     1621 agctcctcgg atgttattga agtttattcc ggtggcgtgc ttgatgttag tggtggtacg
     1681 gcaacaaatg ttacccagca cgatggtgca attttaaaaa ctaacactaa cggtacgacg
     1741 gtgagcggta cgaatagtga aggtgcattc tccatccaca atcacgtggc agacaatgtg
     1801 ttgctggaaa acggtggtca tttagacata aacgcatatg gttcggcaaa caagacgatt
     1861 attaaagata aaggaacaat gtcagtttta accaatgcta aagctgatgc gacccgaata
     1921 gataatggcg gggttatgga tgttgcagga aacgcgacaa ataccataat taatggtggc
     1981 acacagaata ttaataatta tggcatagcc acaggcacca atatcaacag cggaacgcaa
     2041 aatatcaaaa gcggcgggaa agctgacaca acaattatat cctccgggag ccggcaggtt
     2101 gttgagaaag atggtacggc aattggcagc aatattagcg ccggaggctc gctgattgtc
     2161 tataccggcg gtattgcaca tggggttaac caggagacgg gcagtgcttt agttgccaac
     2221 acgggtgcag ggactgatat cgaaggatac aacaagctct ctcacttcac tattaccgga
     2281 ggggaggcta attatgttgt gctggaaaat accggcgaac tgacggtagt ggctaaaacc
     2341 tcggcgaaaa atactaccat tgatgctggc ggtaagctga ttgtccagaa ggaggctaaa
     2401 acagatagca ccagacttaa taatggcggc gttctggagg ttcaggacgg tggtgaggct
     2461 aagcatgttg agcaacaatc cggcggcgca ttaattgctt ccacgacctc cggaacactt
     2521 atcgaaggaa ccaacagtta tggtgatgct ttctacatca ggaattcaga agctaaaaat
     2581 gtagtgctgg aaaacgctgg ctcattaaca gtcgtcactg gttcccgggc agttgacacg
     2641 attattaatg ccaacggcaa aatggatgtt tatggaaaag atgttggcac tgtactcaat
     2701 agtgctggca cccaaacaat atatgccagt gccacttctg ataaagcaaa tatcaaaggt
     2761 ggcaagcaaa cggtatatgg tttagccact gaagcaaata tcgaaagtgg tgaacaaatt
     2821 gttgatggtg ggtcaacaga gaaaacacac atcaatggtg gcacgcaaac cgttcagaat
     2881 tatggtaagg cgatcaatac cgatatcgtc tctggcctac aacaaattat ggcaaacggg
     2941 acagcggaag gttccattat taatggcggt tcacagatag ttaatgaggg cggtctggct
     3001 gaaaactcgg tgcttaatga tggcggcaca ctcgatgtgc gggagaaagg cagcgcaacg
     3061 gggatacagc agagtagcca gggcgcgttg gttgcaacca ccagggcgac gcgggtcaca
     3121 ggaacacgcg cggatggcgt cgcgttcagc atcgagcagg gtgcggcgaa caatatcctg
     3181 ctggcaaatg gcggagtgtt aaccgtggag tcagacacct cttctgacaa aacacaggtc
     3241 aatacgggcg gacgggagat cgtcaaaaca aaagccactg cgacaggcac gacgctcacc
     3301 ggcggtgaac aaattgtcga gggtgtggcg aatgagacaa caattaacga cggcggaata
     3361 caaacagttt cagctaacgg agaggcaata aaaacaacga tcaatgaagg cggtacgctg
     3421 acagtcaacg ataatggcaa agcgacagat atcgtccaga acagcggtgc cgctctccag
     3481 acgagcacgg ctaacggtat tgaaatcagc ggtactcacc agtacggcac tttttccatt
     3541 tccggcaatt tagcgaccaa tatgttgctg gaaaatggcg gtaatttatt ggtattagca
     3601 ggtaccgaag ctcgcgactc cacggttggc aagggggggg caatgcaaaa ccagggtcag
     3661 gactccgcca caaaggttaa ctctggtggg caatataccc ttgggcggtc aaaagatgag
     3721 tttcaggctc tggcccgggc agaagatctc caggttgctg gcgggacagc aatcgtctac
     3781 gcaggtacgc tggcggatgc atcggtcagt ggcgcgacag gaagcctgtc gttaatgacg
     3841 ccacgggata atgttacgcc agttaaactc gaaggggcga tccggattac cgatagcgcg
     3901 acattaacta tcggcaatgg cgttgatacg acgcttgccg acctgacggc tgccagccgg
     3961 ggcagtgtct ggcttaacag caataattcc tgtgcaggca ccagcaactg cgagtataga
     4021 gtaaacagtt tgctacttaa cgacggtaat gtttatttat cagcacaaac agcagcgcct
     4081 gccacaacta acggtatata caatacgctg acaaccaatg aactttccgg tagcggtaat
     4141 ttctacctgc ataccaacgt tgcaggctct cggggcgatc aactggtcgt caacaacaac
     4201 gccactggta attttaaaat ctttgttcag gataccggcg tcagtcctca gtctgacgac
     4261 gcgatgacgc tggtgaaaac agggggaggg gatgcttcgt tttcgctggg caatactggc
     4321 ggtttcgttg atcttgggac ctatgagtat gtcctgaaaa gcgatggcaa cagcaactgg
     4381 aacctgacca atgatgtcaa acccaacccg gatcccaacc caaatcccaa cccaaatccg
     4441 aagccggatc caaaaccaga cccaaaaccg gatccgaaac cagacccgac tcccgagcca
     4501 acgccgacac ccgttccgga gaaacgcatc acgccttcta ccgcagccgt actcaatatg
     4561 gcagcaacat taccgttggt atttgatgct gagctaaaca gtattcgcga gcggttgaac
     4621 ataatgaaag cgagtccaca caacaataat gtctgggggg cgacgtataa cacccgtaat
     4681 aatgtcacgg cgagtatacc ttttattcgc cagtttaatc tgttcatcaa caagaacagc
     4741 catttgaaaa agatcagagt ttcagacgtg atgaaaatcg ctgattctga ctaagaccaa
     4801 cctccattag ctctggcgtt cccagcttcg gtctttttcc tgctgattca tttgtatagt
     4861 catttccatc aaaacggcac agaggaaacg actatgaatc agcacaacac ccgccttatt
     4921 cgtttaccag aggttctgca tcgaactgga tacggtaaag cttggatcta tcacctcatc
     4981 aaccaaaatc gttttccttc ccccgttaaa atcggtgcgc gctctgttgc tttcattgaa
     5041 agtgaagtag acgaatggat tcaattaact atcgaaaatt caagaaagca tgttgcctga
     5101 aataaaatga cagtcttctt atttatgagg attaattctc agatttgtaa agtttaaatc
     5161 ttaaaaaaat ctatatttaa cgtaattctt tacgtttttt ctggcagagc ttttgctata
     5221 aggcttagag atggttttct taatatcggg tatggataca gcccactagc cgtacctgtt
     5281 tatttga
//
LOCUS       DAZFTN010000213         5162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFTN010000213 DAZFTN010000000
VERSION     DAZFTN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..3983
                     /gene="espP"
                     /locus_tag="ACHKV2_004943"
     CDS             81..3983
                     /gene="espP"
                     /locus_tag="ACHKV2_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO6942659.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4239..4720)
                     /locus_tag="ACHKV2_004944"
                     /pseudo
     CDS             complement(4239..4720)
                     /locus_tag="ACHKV2_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1552 a         1133 c         1167 g         1310 t
ORIGIN      
        1 gattacaatg ttcacgactg tcagagacac acctctgata cattagaaaa accaatgttt
       61 cccttaaaaa tggagcttat atgaataaaa tatactctct taaatacagc catattacag
      121 gagggttaat cgctgtttct gaattatccg gcagagtatc atcaagagca actggtaaga
      181 aaaaacacaa acgcatactt gcattatgtt ttttaggctt attacaatcc tcatattctt
      241 ttgcgtcaca gatggatatt tcaaatttct acatccgtga ctatatggat tttgcacaga
      301 acaagggcat atttcaggct ggcgcaacaa atattgaaat agtgaagaaa gatggctcca
      361 ccctgaaact accggaagta ccatttcctg acttctcacc ggttgcaaac aaagggtcaa
      421 ccacatctat tggtggtgca tacagtatca cagccacaca caatacgaaa aaccaccact
      481 cagttgcgac gcaaaactgg gggaacagca cgtacaaaca aactgactgg aatacttcac
      541 atcctgattt tgcagtatcc cgacttgaca agtttgttgt tgagacccga ggtgcgactg
      601 aaggcgcaga tatttcgtta tcaaaacagc aggcacttga acgttacggg gttaattata
      661 aaggagaaaa gaaacttatc gcattcagag ccggctctgg tgtggtatcc gttaaaaaaa
      721 atggacgcat aactccattt aatgaggttt cttataagcc agaaatgtta aatggctctt
      781 tcgttcacat tgatgactgg agtggatggc tgatattaac caacaaccag tttgatgagt
      841 ttaataacat tgcctctcag ggtgacagcg gttcagcact gttcgtctat gataaccaaa
      901 agaaaaagtg ggttgtcgct ggaactgtct gggggattta taattacgcc aatggcaaaa
      961 accacgcagc atacagtaaa tggaaccaga caaccattga caacctgaag aacaagtatt
     1021 cttacaacgt ggatatgtca ggggctcagg ttgcaaccat tgaaaatgga aaactgacag
     1081 gcactggctc agacaccacc gatataaaaa ataaggactt aatatttact ggcggtggag
     1141 atatcctcct gaaatcctct tttgataatg gtgctggcgg tcttgtcttt aatgataaaa
     1201 agacctatcg agtaaacggg gatgatttca cctttaaagg tgccggtgtt gatacaagaa
     1261 acggcagcac cgttgagtgg aatatccggt atgataataa agacaacctt cacaaaattg
     1321 gtgatggcac attagatgtc cgaaaaaccc agaacaccaa cctgaaaaca ggtgagggtc
     1381 ttgtcattct tggagctgaa aaaacattca ataatatcta cataaccagt ggtgatggaa
     1441 ctgtccgact gaatgcagaa aatgcactgt ctggcggtga atacaacggt attttctttg
     1501 cgaaaaatgg cggaactctt gacctgaacg gatataatca gtctttcaat aaaattgctg
     1561 caactgattc aggtgctgta ataaccaata cgtcaaccaa aaaatccatt ttatccctga
     1621 ataatactgc tgactatatc tatcacggta acataaacgg gaatctggac gtacttcagc
     1681 atcatgagac gaaaaaagag aaccgtcgtc ttattcttga tgggggcgtg gacacaacaa
     1741 atgatataag cctgcgtaat acacaactgt ccatgcaggg acatgccact gaacatgcca
     1801 tttatcggga tggagctttc tcttgttcac taccagctcc tatgcgcttt ttgtgtggca
     1861 gtgattatgt tgcaggaatg caaaatacag aagctgatgc tgtaaaacaa aacggaaatg
     1921 cctataaaac caacaatgct gtctctgatt tatcgcagcc agactgggaa accggaacat
     1981 tcagatttgg aacgctacat cttgaaaatt ccgatttttc tgttggtcgt aatgcaaatg
     2041 taatcgggga cattcaggcc agtaaatcaa acattactat tggtgacact acagcatata
     2101 ttgatttgca tgctggtaaa aatattaccg gtgatggttt tggcttccgc cagaatattg
     2161 tgcgtggaaa ctcacaagga gaaacgctgt ttacaggagg gatcacagca gaagacagca
     2221 ctatcgttat taaagataaa gcaaaagcat tattttcaaa ttatgtatac ctgctgaaca
     2281 caaaagcaac catagagaac ggtgctgatg tgacaactca aagtggtatg ttctccacga
     2341 gcgatatcag catctctggt aatctgtcca tgacaggcaa tcccgacaaa gacaataaat
     2401 tcgagccctc aatatatctg aatgatgctt cttatctact gactgacgac tccgccagac
     2461 tcgttgccaa aaataaagca tctgtggtgg gagatataca ctccactaaa agtgcatcca
     2521 tcatgtttgg tcatgatgaa agcgacctct cgcagttgtc tgacagaacc tcaaaagggc
     2581 ttgcacttgg tcttttaggt ggctttgatg tctcatatcg cggttcagtc aatgccccgt
     2641 cagcatctgc cactatgaac aacacctggt ggcaactaac cggagattct gcgctgaaaa
     2701 cactgaaaag tacaaacagc atggtctatt tcactgacag cgcaaacaat aagaaattcc
     2761 atacgctgac ggtcgatgag ctggcaacca gcaacagcgc ctatgcgatg cgtacaaacc
     2821 tttctgaatc agacaaactg gaggtcaaaa aacacttgtc tggtgagaac aatattttac
     2881 tcgttgattt ccttcagaaa ccaacgcctg aaaaacaact gaatattgaa ctggtaagcg
     2941 cgccaaaaga caccaatgaa aatgtcttta aagccagtaa acaaaccatt ggtttcagtg
     3001 atgtaacgcc ggtcattaca accagggaaa ccgatgacaa aataacatgg tcactgacag
     3061 gctataacac ggtagcaaac aaggaagcaa cccggaatgc cgccgccctg ttctctgttg
     3121 actataaagc gtttctgaac gaggtcaaca acctgaacaa acgtatgggt gacctgcgtg
     3181 atatcaacgg cgaagccggt gcatgggcac gcatcatgag cggtaccggc tctgccagtg
     3241 gtggtttcag tgacaactac acgcacgttc aggtcggggt cgacaaaaaa cacgagctgg
     3301 acggactgga tttgtttacc ggtttcactg tcacacacac tgacagcagt gcctccgccg
     3361 atgttttcag tggtaaaacg aagtctgtgg gggctggcct gtatgcttcc gccatgtttg
     3421 attccggtgc ctatatcgac ctgattggca agtatgttca ccatgataat gagtacactg
     3481 caacctttgc cggactcgga acccgtgatt acagcacgca ttcatggtat gccggtgcag
     3541 aagcgggcta ccgctatcat gtcactgagg atgcctggat tgagccacag gctgagctgg
     3601 tttacggttc tgtatccggt aaacagtttg catggaagga ccagggaatg catctgtcca
     3661 tgaaggacaa ggactacaat ccgctgattg gccgaacggg tgtggatgtg ggtaaatcct
     3721 tctctggtaa ggactggaaa gtgacagccc gtgccggtct gggctaccag ttcgacctgc
     3781 tggctaacgg cgaaaccgta ttgcgggatg catctggtga aaaacgcatc aaaggtgaaa
     3841 aggacagccg tatgctgatg tccgttggcc tgaatgcaga aatcagggat aacgtccgct
     3901 ttggactgga gtttgagaaa tccgcctttg gtaagtacaa cgttgataat gctgtcaacg
     3961 ctaatttccg ttactcgttc tgatactccg tattcaaaag ggcctgcggg ccctttacgc
     4021 tcagtgcctg ccgccctgat atgtctgaga gacatttccg ggatgtcagt cgactggttt
     4081 acttaactgc gatgactaaa gctggcagta atgccggctt ttttacggcc acatacgtct
     4141 tcctctcatc cggaactgtg atacaaatcc tgcatacagg attgaccgcc ggttcctgca
     4201 aatggcacat tttcagtaag atagcgaagg ttacatttgt cactggttaa cataccgatg
     4261 gtaaaagggg caagcagcgc cagcaattca tcagcccggg ggccaaaagt gtaggccaga
     4321 acgccaccag ttttcgtgtt gtacgcgtac cagagcctgt gctgtcacgc tttactgccg
     4381 ataaaactcc attgctcatc aagttcgcag atgagtgcca tatcagcatg agcgaccggg
     4441 gatgacgtta ttcttcgtgg cgagcgtttt ttaaagtccg aatgacagta ttaatgccga
     4501 ttttcagcgt acgggcagta tcgcgaactc cggcaccgtt gaaaggcatt tcagtgattt
     4561 ggtcttgaac tcccggttta cgggcctcgt aacggtaagt gagctgaaac actcgatgac
     4621 agtcacggca gcgaaatcga tcatgtcctt ttgggttctt cccgtgacgg taaacctgaa
     4681 cggactggca acgggggcaa tgaaggttaa ctgtgaccat taaagaacct taaaagtgac
     4741 tattataccc ctaattcaac taattggagg cattaccatg cattagtgca agcgccttgt
     4801 ggattctttt gctaatggcg gaaacatatt gtacaatatt gcatttatcc ctcaataaat
     4861 aaatgagtta tcagattgca aaaatctgtt atcaaactaa ttcaaataaa aagagattga
     4921 tacgaccaac gtacacaaaa attttcgata ttcgaactaa ataacagctc ctaataccgc
     4981 tacactttag ctatccagtc tgcgcctcag aaatggacga tgccgttgaa ggactggtgt
     5041 atggcaatga gccgctttat tatcgaattc ggtgaccgct tggacggtca cttcggagaa
     5101 aaggcattta cacagcatcc taaacaggtt cagcataggt gatataccgc cttttcattt
     5161 tc
//
LOCUS       DAZFTN010000214         4956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFTN010000214 DAZFTN010000000
VERSION     DAZFTN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..887)
                     /gene="kduD"
                     /locus_tag="ACHKV2_004945"
     CDS             complement(126..887)
                     /gene="kduD"
                     /locus_tag="ACHKV2_004945"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO6942660.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(917..1753)
                     /gene="kduI"
                     /locus_tag="ACHKV2_004946"
     CDS             complement(917..1753)
                     /gene="kduI"
                     /locus_tag="ACHKV2_004946"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO6942661.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2040..3221)
                     /gene="yqeF"
                     /locus_tag="ACHKV2_004947"
     CDS             complement(2040..3221)
                     /gene="yqeF"
                     /locus_tag="ACHKV2_004947"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO6942662.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3476..4705
                     /gene="yqeG"
                     /locus_tag="ACHKV2_004948"
     CDS             3476..4705
                     /gene="yqeG"
                     /locus_tag="ACHKV2_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6942663.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1307 a         1248 c         1069 g         1332 t
ORIGIN      
        1 aattcataaa gttcattaat tgataattga tatggattat ttcataacca tgatatggat
       61 tatgatgatc tagagatata aaaaaccctg ccatgcggca gggtcataaa agtaagagga
      121 atgaattaac gcgccagcca accgccatcc acagcaatgg tataaccatt tacataatct
      181 gaagcgctgg aggcaagaaa cactaccggc cccatcaggt cactcggcag tccccaacgg
      241 ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg cagttgttgg
      301 gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
      361 cattcattcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa
      421 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
      481 ttgccttgcg cgatgaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
      541 ttcaggttca tgacatcgtc ccagtccttt tcgctgaact cgagagcatc ttcgcggcga
      601 atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc
      661 agcagtccag gaataccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
      721 cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg
      781 tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca
      841 gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat
      901 tcgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
      961 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
     1021 gaatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
     1081 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     1141 aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacccggag
     1201 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
     1261 tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     1321 ccggtgtgac ttttttggta ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     1381 tcgccggagt gccggtatca atactagcaa agacaacttc ttttgcgcct ttacccacat
     1441 acagggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     1501 cgccaatatt gataacacct aactcgcgac gttcaaggaa ataacttacg ccgagttgtt
     1561 tacccacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaataataa
     1621 tacggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     1681 aaaattcatt gcgcagcccc tgggtttcca gcgtttttgc gtgcgcactg tggatgctct
     1741 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     1801 aaacactgtt tcgttttctc tataagggga tgatatgccc atgggatcgg gttttcaatt
     1861 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
     1921 gaatctgtca tgagaatgga aaaatcaggg agcccggaaa atgtatgtcg acttgccagc
     1981 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
     2041 tattcgtcac gttcaatggt caatgccaca ccctggcccc cgccgataca aagcgttgcc
     2101 agtcctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
     2161 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
     2221 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     2281 tcgataagat caacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
     2341 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
     2401 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
     2461 gctgcgccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag actatcaaat
     2521 gaaggattta aacgggctaa accttctgca ctggcgtcag tgtgtggctg ttcatcggta
     2581 tcaacaacca acgtctgacc gttactttgg gtcattaccg ggacgatctc atctttaaat
     2641 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     2701 aattgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     2761 tcattgaagg catcccataa cccatcatgc acaagactgt caaccagctg gctattgcca
     2821 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     2881 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     2941 gctttaagcc cggaaccgca gacgtcatta atcgtgattg cagaaacgct attaggcaga
     3001 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     3061 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
     3121 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccgcg aaagcagccg
     3181 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     3241 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
     3301 tttatcacaa aggaaatata cctgagcagt agtcagagtc ataactggca cgtaaggttt
     3361 gcaaccacta ccccaccaat agaggggtag atagggcgtt aatctcccat acttaacctg
     3421 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     3481 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     3541 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
     3601 tactgctctg gtcgcctggc ctttaacata ctggccacat aaagctttat gccagttcat
     3661 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
     3721 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     3781 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
     3841 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
     3901 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
     3961 ctatttctta tttctttcca tttaccttgt aggtagttgg caacctgatc tattaacaac
     4021 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
     4081 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
     4141 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
     4201 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
     4261 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     4321 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     4381 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
     4441 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
     4501 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
     4561 cggcccgctc attgccatga tacttttcat catgcctacg ctatcaacat atctcatccc
     4621 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     4681 cgtatcggta atgttcttta gctaatatcc attgtgaatg gttcaaaatt gctacaattt
     4741 tgaaccattc cagttttcat aatgtgcatt tgttattatt ccgctctatc acttctctcg
     4801 tcatatccta gtattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
     4861 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
     4921 gcgatataga tattctcata ccatgagata gttatc
//
LOCUS       DAZFTN010000215         4951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFTN010000215 DAZFTN010000000
VERSION     DAZFTN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            194..757
                     /locus_tag="ACHKV2_004949"
     CDS             194..757
                     /locus_tag="ACHKV2_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942664.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            839..3478
                     /locus_tag="ACHKV2_004950"
     CDS             839..3478
                     /locus_tag="ACHKV2_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6942665.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            3501..4259
                     /locus_tag="ACHKV2_004951"
     CDS             3501..4259
                     /locus_tag="ACHKV2_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6942666.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            4270..4770
                     /locus_tag="ACHKV2_004952"
     CDS             4270..4770
                     /locus_tag="ACHKV2_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942667.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            4767..>4951
                     /locus_tag="ACHKV2_004953"
     CDS             4767..>4951
                     /locus_tag="ACHKV2_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942668.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVP"
BASE COUNT         1267 a         1235 c         1305 g         1144 t
ORIGIN      
        1 aaatataatt tgaaacaata aagacaaaat atcaccatcc gcccacacca aagaatatct
       61 tcttatgcga cctattttct tgttagttag atcggatagc ttattcaatg gctgggtata
      121 aataattgct attgactgaa tcaatgtatt gcaatgttta tatttttaat tatttatggt
      181 ttaaggacaa ataatgagta agtttgtaaa aacagctatt gctgcaacaa tggtaatggg
      241 tgcgttcgct tctacttcaa caatcgccgc tggcaacaat ggtacagcac gtttctacgg
      301 caccattgaa gattccccgt gctctatcgt tcctgatgat cacaaactgg aagttgatat
      361 gggtgacatt ggttcaggga tcctgaaaaa taacgggact tctacaccga aagctttcca
      421 gatccatctg caagactgtg tgtttgacac ccagacaacg atgaccacta ccttcaccgg
      481 taacgcgtct tctaccaaca gcggcaatta ctacaccatt tacaataccg atactggtgc
      541 ggcatttaac aatgtcagcc tggccattgg tgacgctcag ggaacctctt ataaaagcgg
      601 cgcgggtatc gaacagaaaa tcgtaaacga tacggcgacc aacaaaggca aagcgaagca
      661 gacgctggac tttaaagcct ggctggtggg cgctgctgat gcgccagatt taggtaattt
      721 tgaagccaac acgaccttcc agattactta tctctaagtg atacccccat agctttcggg
      781 ctatggggtt tttaaatgga attcgcgcaa attataataa ttacggatgg atacgtgtat
      841 gcctgaccat tctcttttcc gactgcgggt acttccctgc tgcgttgcat tggcaatgtc
      901 cgggagttat gtcaatgcct gggctgaaaa tgaaattcag tttgattccc gttttctgga
      961 gttaaaaggc gacacaaaaa tcgatctgaa gcgattttcc agccagggtt atgtcgaacc
     1021 tgggaaatac aatttacagg ttcaactaaa taaacagccg ctgacggaag aatacgatat
     1081 ttactggtac gcctctgaga acgatgccag taaaacctat gcctgcctga cgcctgaact
     1141 ggtcgcgcag tttggcttaa aagaggatgt ggcaaaaaac ctgcaatgga tccacgacgg
     1201 caaatgcctg aaacccggtc aactggaagg cattgatatt aaagctgacc tgagtcagtc
     1261 agcgttagtc atttcattac cccaggctta ccttgaatat accgacatca actgggatcc
     1321 gccttcacgc tgggatgacg gtatatctgg tttaattgct gactacagta ttaccgccca
     1381 gacacgacat gaagaaaatg gcggggatga cagcaatgaa attagcggta acgggacggt
     1441 tggggtgaac ctcggcgcat ggcgtcttcg tgccgactgg cagactgatt atttgcatag
     1501 taaaagcaat gatgacgatg ttatcaacgg tgatgacacg caaaaaaact gggagtggag
     1561 ccgctactac gcctggcgag ccttaccgtc gctaaaagcc aaacttggcc ttggcgaaga
     1621 ctacctgaat tctgatattt tcgacggctt taactacgtg ggtggcagta tcagcaccga
     1681 cgatcaaatg ttgccgccga atctgcgcgg ctatgcgccg gatatctccg gcgtggcgca
     1741 caccaccgcg aaagtgaccg tcagccagtt gggccgcgtc atctacgaaa cccaggtccc
     1801 ggcggggccg ttccgcatcc aggatcttgg cgattcggtc tccggtacgc tgcatatccg
     1861 cattgaagaa cagaacggtc aggtgcagga atatgacatc aacaccgcct cgatgccgtt
     1921 cctgactcgc cccggccagg tgcgctataa actgatgatg ggccgcccgc aggagtgggg
     1981 gcaccacgtg gaaggcggtt tcttctccgg cggcgaagct tcctggggga ttgccaacgg
     2041 ctggtcgcta tacggcgggg cgctggcaga tgaacactat cagtcggcgg cgcttggcgt
     2101 cggtcgcgac ctgtctgtgt ttggtgcggt ggcctttgat atcacccact cgcatacccg
     2161 tctggataaa gagaccgcct acgggaaagg ttcactggac ggcaactcgt ttcgcctgag
     2221 ctattccaaa gacttcgatg aactgaacag ccgcgtcact tttgccggat accgcttctc
     2281 ggaagagaac ttcatgacca tgagcgagta tctcgatgcc agcgacagcg aaatggtgcg
     2341 caccggcaac gacaaagaga tgtacaccgc cacctataac cagaacttca gggatgccgg
     2401 tgtgtctgtt tatctcaact acacccgcca tacctactgg gatcgcgacg aacagaccaa
     2461 ctacaacgtc atgctctcgc actacttcaa cctgggcagt atccgcaaca tgagcatttc
     2521 catgaccgga taccgctacg agtatgacaa ccaggccgat aaaggtgtgt acatatcgct
     2581 cagtatgccg tggggtgaca gcagcaccat cagctataac ggcaactacg gcagcggttc
     2641 ggacagcagc caggtggggt atttcagccg tgtcgatgac gcaacccatt accagttgaa
     2701 cgtaggcacc agcgacaatc actccagcgt tgacggttat tacagccacg acggatcgct
     2761 ggcgcaggtc gatctcagcg ctaactacca tgaagggcag tacacctcgg cgggtatttc
     2821 cttacagggc ggcgcgacgc tcaccgcaca aggtggcgcg ctccaccgta cccagaatat
     2881 gggcggtacg cgtctgctga ttgatgccga cggtgtggct ggtgttccgg tggaaggaaa
     2941 tggcgcggcg gtttacacca atatgttcgg taaggcagtg gtggcagacg tcaacaacta
     3001 ctaccgcaac caggcgtata tcgacctaaa caacctgccg gaaaacgccg aagccaccca
     3061 gtccgtggtg caggccacgc ttaccgaagg ggccattggc taccgtaagt tctcggtgat
     3121 cagcgggcaa aaagcgatgg cggtgctgcg tctgcaagat ggcagttatc cgccgtttgg
     3181 cgcggaagtg aaaaacgaca gcgcgcagaa cgtcggtctg gttgacgatg acggcaacgt
     3241 ctacctcgcg ggcgtaaaac ctggcgagca tatgatcgtt tcatggggcg gtgtggccca
     3301 ctgcgatatt catctgcctg acccgctgcc agccgatctg ttcaatggcc tgttattacc
     3361 atgccagcaa acaggggcga tatctccttc gatgcctcat gaaattaagc cggtgatcca
     3421 ggagcagacc cagcaggtga tgccaacgga agcgccagta tcggtatcag ccaattaata
     3481 acgtgattaa ggaatgatcc atgacgaatc tattttgctc tgccagaacg gccgcaacga
     3541 cgctggcgtt actgataact gccgccagcc tgtcagtaca ggcctctgtc acgccggacc
     3601 gtacacgcct gatttttaac gaaagtgata aatcaatcag cgtcacgctg cgcaataacg
     3661 acccgaaaat gccgtatctg gcacaaagct ggatggaaga tggacagggt aacaggatta
     3721 gctcgccact gacggtcctg ccgccagtgc agcgcatcga ttccatgatg aacggtcagg
     3781 tgaaggtaca gggcatgccg gatatcaaca agctgcctgc cgaccgcgag agcgtgttct
     3841 atttcaacgt gcgtgagatc ccgccgaaat cgaacaaacc taacacgcta cagatagcgc
     3901 tgcagacgcg cattaagttg ttctggcgac caaaggcgct ggaaaacgtc agcatgaaaa
     3961 acccgtggca gcataaggtg accttaaccc tcagtggtca ggagtttacc gtcaataacc
     4021 cgacgccgta cttcgtggtt atcagtaacg ccagtgcgca gaaaaatggc aatccggcag
     4081 cgggattttc ccccatggtg atatcgccaa aaacctctgt gccgttaaaa gtcaaaatgg
     4141 atagcgtccc ggtgctgacc tatgtgaatg attatggtgc gcgtttgccg ctgttcttcc
     4201 gctgtacggg aaatacctgt caggtggacg aagagcaaaa cagcaaaggc cagggatagg
     4261 agcaatagca tgaaaggagc ttattcggcc tgggcgtggt tattgctgtg tagcattgtg
     4321 agcacgcctg taatggcggg gagtaaatcg gttgcgatgg tcttgcgtgt gctcgttgat
     4381 gccccgccgc cctgtacggt aacgggtgcg agcgtggagt ttggtaatgt cttcatttcc
     4441 aagatcgatg gggtaagtta caaacgcccc attgactact cgcttgtgtg caacaatctg
     4501 gcaatggacg acctgcgctt aaatatgcag gcaaccacaa ccgtaattaa cggagaaacg
     4561 gtaatcgata cgggtattgc cggttttggt atccgtattc agaaagtaag cgatcactcc
     4621 attttggatt taacgcctgg cgcatggtta ccgtttaact tttcatctgg cgcactggct
     4681 ctggaagcgg ttcctgtcgt gcagagcggt gtatcgctaa cggcggcgga atttagcgcc
     4741 agtgccacaa ttgtggtgga ttatcaatga aacggatctc tctcttaatg ttgtggagct
     4801 ttagctttat ggcgctctcg aatgtctctt ttcatggtta tttagtgcag ccacccaatt
     4861 gctctattag taatgggcaa aatatagagt tgacgtttcg ggacgtgaat attgatgaca
     4921 ttaacggtag caattacgaa caggtcgtgc c
//
LOCUS       DAZFTN010000216         4945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFTN010000216 DAZFTN010000000
VERSION     DAZFTN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHKV2_004954"
     CDS             <1..882
                     /locus_tag="ACHKV2_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6942669.1"
                     /translation="AQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLLQITENIR
                     SEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSGTGQRRWE
                     ELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDLLTVLPTR
                     LDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDFDTPWCQP
                     ESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSPTDDDELP
                     EVTGPAWIVDKVAHYGG"
     gene            879..1190
                     /locus_tag="ACHKV2_004955"
     CDS             879..1190
                     /locus_tag="ACHKV2_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942670.1"
                     /translation="MKYDGDGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSERRRLRYSTVGLTRYRT
                     R"
     gene            1266..1949
                     /locus_tag="ACHKV2_004956"
     CDS             1266..1949
                     /locus_tag="ACHKV2_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6942671.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            1950..2171
                     /locus_tag="ACHKV2_004957"
     CDS             1950..2171
                     /locus_tag="ACHKV2_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942672.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            2185..2619
                     /locus_tag="ACHKV2_004958"
     CDS             2185..2619
                     /locus_tag="ACHKV2_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6942673.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            2665..3441
                     /locus_tag="ACHKV2_004959"
     CDS             2665..3441
                     /locus_tag="ACHKV2_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942674.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLAMLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRSVSCRVAEDAQRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            3854..4279
                     /locus_tag="ACHKV2_004960"
     CDS             3854..4279
                     /locus_tag="ACHKV2_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6942675.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYMFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            4326..4748
                     /locus_tag="ACHKV2_004961"
     CDS             4326..4748
                     /locus_tag="ACHKV2_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6942676.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLPD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSHQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            4745..4936
                     /locus_tag="ACHKV2_004962"
     CDS             4745..4936
                     /locus_tag="ACHKV2_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942677.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT         1044 a         1337 c         1519 g         1045 t
ORIGIN      
        1 gcacagatcg ctgagattaa acgactggcc agcggtgcag tcacaccgct ttatcgccgc
       61 gccacaaatg aaggtattca gctgtttctg gccggaagtg ccggacttct gcagatcact
      121 gaaaatatcc gctctgagca gtgccccgga gtgacggctg ccggacgtgg tgctgtatca
      181 ccggaaaata tcgcgttcac ccgctggctg acacacctgc agaacggtgt actgctggat
      241 gagcaaaact gcctgatgct gcatgaactc tggctgcaga gcggtactgg acagcgtcgc
      301 tgggaagagt taccggatga tgtcagggaa actatcactg tgcatttcac cgcaaaaaga
      361 ggtgactggt gtgacatctg gggtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt
      421 gacaacgtac tgccggaaaa aaccatgccg tttgacctgc tgacggtttt gccgacccgc
      481 ctggatattg aagtgaatgg ctttaacggc ggtgttctga acggtgttcc ttctgcatat
      541 cactggtata cagaacggta tggcgtgaag tggccttgtg ggtatgacct gaatatcagt
      601 agtcagggag acaacttcat tcaggttgat ttcgacacgc cgtggtgtca gccggaaagc
      661 gacgttattg cagaattaag ccgccgtttc agctgcacgc tggagcactg gtatgccgaa
      721 cagggctgtg atttctgtgg ctggcagttg tatgagcgcg gagagcttgt tgatgtgctg
      781 tggggggagc tggaatggtc ttccccgaca gatgatgatg agctgccgga agtcaccgga
      841 cctgcgtgga tagtcgacaa ggtggcgcat tacggtggat gaagtatgac ggagacgggc
      901 gggcaaccgc ccgtttcttt tccggtaaag gatgtcgcca tgctcctctt tttactgcgc
      961 cggctgacgc ggcgcggcat aaacgctgcc tgtggtcagt gtcccgcgtc cggcgggcac
     1021 gggacgggcg cttttaccgc tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc
     1081 ccttttctga tgaacgccct tcaagccgct ttcgcggcat aaccttgccg tcagaaagac
     1141 gacggctgcg gtattccacg gtcggcctga cccgttaccg gacgcggtga acagcccaca
     1201 ggcagcgggg aacgggcacc acaggggtgc cctcccggtg ccctctgtaa gagaaggagt
     1261 ttcgtatgtc ccgttttgtc ctcggtaact gcatcgatgt tatggcccgt attcctgata
     1321 acgccattga tttcatcctc accgacccgc catatctcgt cggtttccgt gaccgttccg
     1381 ggcgcaccat cgccggcgat aaaaccgatg agtggctgca accggcctgt aatgaaatgt
     1441 tccgcgtgct gaaaaaagac gcgttaatgg tgagcttcta cggctggaac cgcgtcgatc
     1501 gctttatggc cgcctggaaa aatgcgggat tcagcgttgt tggtcacctg gtcttcacca
     1561 aaaactacac atcgaaggcc gcatatgtgg gctatcgcca cgaatgcgcc tacatcctgg
     1621 caaaaggccg tccacgtctg ccacaaaacc cgctgccgga cgtgctgggc tggaaatatt
     1681 cgggcaatcg ccatcacccg acggaaaagc ccgttaccag cctgcaaccg ctgattgaga
     1741 acttcacaca cccgaacgca attgtgctgg acccgtttgc aggcagcggc tcaacctgcg
     1801 tcgccgccct ccagtccgga cgccggtata tcggtatcga gctgcttgag cagtatcacc
     1861 gtgccgggca gcaacgcctt gccgccgtgc aacgggccat gcagcagggg gccgcgaatg
     1921 atgactggtt tatgccggag gctgcgtaaa tgaactatgc aggacacgaa aaactgcgcg
     1981 ccgaagtggc ggaggtggcc aatgccatgt gcgacctgcg tacaaccatg aatgagatgg
     2041 agcagcggta cagctttaat gccgacaccc tgccggaacg tctggtgcgt cagacgctgt
     2101 ttcgcgcaaa ccgcctcctg atggaggcat ataccgaaat tctggaactg gaagcgtgct
     2161 ttaaagattg agaaggagac gagaatgtac ggaacatgcg aaacgctatg ccgggagctg
     2221 gcagtaaagt atccgggaga catgccgctg atgctggtta tctggtcccc ggaagagatt
     2281 caggccctcg ctgacggaat ggatatttcc ctgtccgatc atgaaatcag aaccgtcctg
     2341 gcgcgcctgg aggacatccc ggaagaccag cggactgaat ccggtatttc ttccggcgtg
     2401 gcgatggaga tcatcaataa cgtgagcgaa aaccgccagg tgaccgtccc tgctgaactg
     2461 ctggcgtccc tgattcagac cgctgaacag gcattgtgga aacgtgaatg ggccgcccgg
     2521 gatcatggcc tcgccgtccc ggaatgcgtc acccgccgtc aggcggtgat taatcaggcc
     2581 cgcaccctgc tgaaaaacaa tagacacgaa aacgactgat gttatcgccg cctccgggcg
     2641 gcgattcagg gagaacgatt aatcatgaac gaaaacacaa cactgaacgc actgatttgt
     2701 cgtcacgccc gcaacctgct gctggcgcag ggctggccgg aagagacgga tgttgaccag
     2761 cggaacccga agtatccggg ctggatcagc atttatgttc tgctggatgc gccccggctg
     2821 gcgatgttac tcatcaaccg tcacggcggc gtactgccgc cgctcctggc ctccgccatt
     2881 cagaaactga ccggaaccgg ggcggaactg gtactgtctg gcagtcagtg gcagtcgctg
     2941 ccggtacttc cggcagacgg aacacaggtg tttttcccgt atgccggaga atggctgacc
     3001 gaagacgaaa tcagggcagt tcttgatgca gtgcgcgatg cggtacgcag tgtcagttgc
     3061 cgggtggcag aagatgcgca gcgtatcagg gcggcgctga ccacaaccgg tcagacgttg
     3121 ctgacccgcc agacgcgccg ctttcgcctg gttgtgaagg aaagcgatca cccctgctgg
     3181 ctcgatgaag acgacgaaaa cctgcccgtg gtgctcgatg ccatcctgaa ccggggagca
     3241 cgtttttcgg cggtggaaat gtatctggtc agtgagtgtg tggagcatat cctgtccagt
     3301 gggctggcct gcgatgtgct gcgtataccg gatgaaccgc cccgccggtg gtttgaccgt
     3361 ggtgttctgc gggaggtggt ccgggaagcc cgggccgaaa tccgcagcat ggcggatgcc
     3421 ctggcaaaaa tcaggggctg atgatggcgg ggaaaatccc cgcctttttc ctgccggtcc
     3481 cggacagagc acaaaggacg tcgccgccgg ctccgcttta cccggccatg cggggcatgg
     3541 ccttatgggt tttcagttat gtggcctcag cgccgtgtgc gggctgtgcc gtgcctccat
     3601 cttagccggg ctggcgttga tgcaagggta cgcttcgccg ctgcggtcac ccggtccctc
     3661 cttcccgtct gccgtgattt tccggtcgtt cgcccgctca ggtttcgcgg cctcaccctg
     3721 caactcccgc cagccgtgtg cgtcaggctg cggcttccct tgcgcccctg catccccgcc
     3781 tgttcgcccg gcttttatgg aggcacggca ccgcccggtg tcgcggaata tgtgaacaac
     3841 ggaggaaaac atcatgcaat atgcgaaacc tgtcactctg aacgttgaag agtgcgaccg
     3901 tctctccttt ctgccgtata tgtttggcaa cgattttctg tatgccgagg cgtatgtgta
     3961 cgcgctggcg caaaaaatga tgccggaata tcagggcggc ttctggcact tcatccgcct
     4021 gccggacggt ggcggttaca tgatgccgga cggtgaccgt ttccacatgg tgaacggtgc
     4081 aaactggttt gaccgtaccg tgagtgctga tgcctgcggc atcattctga cttcccttgt
     4141 gattaaccgc cagttgtggc tgtaccacga cagcggtgat gcaggactga cccagcttta
     4201 ccggatgcgc gatgcgcagt tgtggcgtca catcgaattt caccctgaat gcaacgcgat
     4261 ttacgcggct ctggactgat taacggacgg ggcggcaatg ccgcccctgt aaaggagacg
     4321 agaaaatgta ctgtactgtt aaagaaatta tccgcgaagt gctggataca gacgtgccgg
     4381 acagtgaatg cgtttttgcc gtggtgctga cccgtgggga tgtgcgtcac atagcccagg
     4441 actggaacct gccagacgat gagctggaaa ccgtcatgca gcggctggac gatgcctttg
     4501 agcatggtgc ggatgtcagc gttgttcacg acgttgttcg tgaactgatg gaagaaaagc
     4561 gcgtcagcca tcaggtgaca gtcccggcgg tgatgctgga aaaagtgatg gcgctggcag
     4621 gcagtgaaat gaagcgcctg tatgccgtcg ggagtgagaa cgggggcgac ggtgacgcat
     4681 tcgtcaggga agaacgcgaa gcgatggacg ttgtgttaca ggcgctggac ggggagcaca
     4741 tgtcatgaat atcagcacag aaacccgcga aattctgcgc aattacaaag ccgtgattaa
     4801 tgcgcggcgt cgtgaaatgg ggcagaaacc gctcaccact gcgcagattg ttgatgaaat
     4861 ctgcgatttt gtggtgaatc agcaggcggt tttcctcggt ggtcactata tccttcaggg
     4921 cagcagaaac aggtgataca gggca
//
LOCUS       DAZFTN010000217         4902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFTN010000217 DAZFTN010000000
VERSION     DAZFTN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..1416
                     /gene="garP"
                     /locus_tag="ACHKV2_004963"
     CDS             82..1416
                     /gene="garP"
                     /locus_tag="ACHKV2_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO6942678.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1432..2202
                     /gene="garL"
                     /locus_tag="ACHKV2_004964"
     CDS             1432..2202
                     /gene="garL"
                     /locus_tag="ACHKV2_004964"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO6942679.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2232..3122
                     /gene="garR"
                     /locus_tag="ACHKV2_004965"
     CDS             2232..3122
                     /gene="garR"
                     /locus_tag="ACHKV2_004965"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6942680.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3219..4364
                     /gene="garK"
                     /locus_tag="ACHKV2_004966"
     CDS             3219..4364
                     /gene="garK"
                     /locus_tag="ACHKV2_004966"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO6942681.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4397..4773
                     /gene="rnpB"
                     /locus_tag="ACHKV2_004967"
     ncRNA           4397..4773
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKV2_004967"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1155 a         1180 c         1401 g         1166 t
ORIGIN      
        1 tcgttacggc ataaatgaaa ctcataaata acatcttccc cataataata aaaatcagca
       61 taagtacccg aggtaaataa aatgattctg gacaccgttg acgaaaaaaa gaaaggcgtg
      121 catacccgct atttaatatt actgattatt tttattgtta ccgccgttaa ctacgccgat
      181 cgtgcaacgc tgtctattgc tggtaccgaa gtggcaaaag agttgcagtt aagtgcggtt
      241 tcgatgggtt acatcttttc tgcttttggc tgggcctact tgctgatgca aatccccggc
      301 ggctggctgc ttgataagtt tggctcgaaa aaagtttaca cctacagcct ctttttctgg
      361 tcgctattca ccttcctgca aggctttgtt gatatgttcc cgctggcctg ggcagggatc
      421 tccatgttct ttatgcgctt tatgctcggc ttctcggaag cgccatcatt cccggcgaac
      481 gcccgtattg tcgccgcctg gttcccgacg aaagaacgtg gtactgcctc cgccatcttt
      541 aactcggcgc aatatttctc gctggcgctc ttttcgcctc tgcttggctg gctgactttc
      601 gcctggggct gggagcacgt ctttaccgtt atgggggtga ttggttttgt gctgacggcg
      661 ctgtggatca aattgattca taacccgaca gatcacccac gtatgtctgc ggaagagctg
      721 aagtttatct ctgaaaatgg cgcggtggtc gatatggacc acaaaaagcc gggcagtgcg
      781 gcagcaagcg gacccaaact gcattacatc aagcaattgc tctctaaccg catgatgttg
      841 ggcgtatttt tcggacaata ttttatcaac accatcacct ggttcttcct cacctggttc
      901 ccgatttatc tggtgcagga aaaaggtatg tcgattctga aagtgggtct ggtcgcctcg
      961 attccagcac tgtgtggttt tgcgggcggc gtgctgggag gtgtcttctc ggattatctg
     1021 atcaaacgcg gtttatccct gaccctggca cgtaagctac cgattgtgct gggaatgttg
     1081 ctggcttcca ccatcatctt atgtaactac accaacaaca ccacgctggt ggtcatgctg
     1141 atggcgctgg ctttctttgg taaaggattt ggtgcgctgg gctggccggt gatttctgac
     1201 accgcgccta aagagattgt tggcctctgc ggcggcgtct ttaacgtctt tggcaatgtt
     1261 gcctccattg tcactccact ggtgattggc tacctggtaa gtgaactgca ctccttcaat
     1321 gcagcactgg ttttcgtggg atgttcagcg ctaatggcga tggtctgcta cctcttcgta
     1381 gttggcgaca ttaaacgtat ggaattgcag aaataagcaa aggtataagc gatgaataac
     1441 gatgttttcc cgaataaatt caaagccgca ctggctgcga aacaggtaca aattggttgc
     1501 tggtcagcac tctctaaccc gattagcact gaagttcttg gtttggctgg gtttgactgg
     1561 ctggtgctgg atggcgaaca tgcgccaaac gatatttcaa cgtttattcc gcagttaatg
     1621 gccctgaaag gcagcgccag cgcgccagta gtgcgagtgc cgaccaacga gccggtaatt
     1681 attaagcgtc ttctggatat cggtttctat aacttcctga ttccttttgt agaaacaaaa
     1741 gaggaagcag agcaggcggt ggcatcgacg cgttatccgc cagaaggcat tcgcggcgtc
     1801 tccgtttctc accgcgccaa tatgtttggc accgtggcgg attatttcgc tcagtcgaac
     1861 aagaacatca ctattctggt ccagatagaa agtcagcagg gcgtagataa cgtcgatgct
     1921 attgccgcta ccgaaggcgt agacggcatc ttcgtcggcc ccagcgatct ggccgcggca
     1981 ttaggccatc tcggcaatgc atcacacccg gatgtacaaa aagcaattca gcacattttt
     2041 aaccgtgcca gcgcgcacgg caaacccagc ggtatcctcg cgccggtcga agccgatgcg
     2101 cgtcgttatc tggaatgggg cgcgacgttt gtggctgtcg gcagcgatct cggcgtcttc
     2161 cgctctgcca ctcagaaact ggctgatacc tttaaaaaat aaccaccacc gcaacaagag
     2221 agatgattga tatgactatg aaagttggtt ttattggcct ggggattatg ggtaaaccaa
     2281 tgagtaaaaa ccttctgaaa gcaggttact cgctggtggt tgctgaccgt aacccagaag
     2341 ctattgctga cgtgattgct gcaggtgcag aaacagcttc tacggctaaa gcgatcgctg
     2401 aacagtgcga cgtcatcata accatgctgc caaactcccc tcatgtgaaa gaggtggcgc
     2461 tgggtgagaa tggcattatt gaaggcgcga agccaggtac ggtattgatc gatatgagtt
     2521 ctatcgcacc gctggcaagc cgtgaaatca gcgaagcgct gaaagcgaaa ggcattgata
     2581 tgctggatgc tccggtgagc ggcggtgaac cgaaagccat cgacggtacg ctgtcagtga
     2641 tggtaggcgg cgacaaggct attttcgaca aatactatga tttgatgaaa gcgatggcgg
     2701 gttccgtggt gcataccggg gaaatcggtg caggtaacgt caccaaactg gcaaatcagg
     2761 tcattgtggc gctgaatatt gccgcgatgt cagaagcgtt aacgctggca actaaagcgg
     2821 gcgttaaccc ggacctggtt tatcaggcaa ttcgcggtgg actggcgggc agtaccgtgc
     2881 tggatgccaa agcgccgatg gtgatggacc gcaacttcaa gccgggcttc cgtattgatc
     2941 tgcatattaa ggatctggcg aatgcgctgg atacttctca cggcgtcggc gcacaactgc
     3001 cgctcacagc tgcggttatg gagatgatgc aggcactgcg tgcagatggt ttaggaacgg
     3061 cggatcatag cgccctggcg tgctactacg aaaaactggc gaaagtcgaa gttactcgtt
     3121 aatgacgtcg tgcccggatg gcgtattgca atccgggcct ggaatccagg ccgaataaga
     3181 gaaacgccct ccggcgtcat gtggtaacag gcataggtat gaaaatcgta atcgccccag
     3241 actcttataa agaaagttta tctgccagcg aggttgcgca ggcgatagaa aaaggatttc
     3301 gggaaatttt tcctgatgca cagtacgttt ctgttcctgt tgccgacggt ggcgaaggaa
     3361 cggtggaagc gatgattgca gccacccagg gttccgaacg tcacgcctgg gttacagggc
     3421 cgctgggcga gaaagtgaat gccagttggg ggatctccgg cgatggcaaa accgcgttta
     3481 ttgaaatggc ggcggccagt gggctggagc tggtacctgc ggaaaaacgc gatccactcg
     3541 tgaccacttc acgcggcaca ggcgagttaa tcctgcaggc gctggagagc ggcgcgacaa
     3601 acattattat cggcattggc ggcagcgcta caaatgatgg cggcgcaggc atggtacagg
     3661 cgctgggggc gaaattatgc gacgccaacg gcaatgaaat tggttttggc ggcggtagtc
     3721 ttaatactct gaatgatatt gatatttccg gcctcgatcc gcgcttaaaa gattgcgtca
     3781 ttcgcgtcgc ttgtgatgtc accaatccgc tggtgggcga taacggcgca tcgcgcatct
     3841 ttggcccaca aaagggagcc agtgaagcga tgattgttga gctggacaat aacctctctc
     3901 actatgccga ggtcattaaa aaagcgctgc atgttgatgt gaaagatgtc cccggcgcag
     3961 gagctgcggg tggtatgggc gcggcgctaa tggcgtttct tggtgcggaa ctgaaaagtg
     4021 gtattgaaat cgtcactacg gcgctgaatc tggaggaaca tattcacgat tgtacgctgg
     4081 tgatcaccgg tgaagggcgt attgacagcc agagtattca cggcaaggta ccgattggtg
     4141 tcgcaaacgt ggcgaagaag taccataaac cggtgattgg cattgcgggt agcctgaccg
     4201 atgatgttgg cgttgtacat cagcatggca ttgatgcggt cttcagcgta ttgaccagca
     4261 taggtacgtt ggacgaagca ttccgcgggg cttatgacaa tatctgccgt gcttcacgta
     4321 atatcgccgc gacactggcg attggaatgc gcaacgcggg gtgacaaggg cgcgcaaacc
     4381 ctctatactg cgcgccgaag ctgaccagac agtcgccgct tcgtcgtcgt cctcttcggg
     4441 ggagacgggc ggaggggagg aaagtccggg ctccataggg cagggtgcca ggtaacgcct
     4501 gggggggaaa cccacgacca gtgcaacaga gagcaaaccg ccgatggccc gcgcaagcgg
     4561 gatcaggtaa gggtgaaagg gtgcggtaag agcgcaccgc gcggctggta acagtccgtg
     4621 gcacggtaaa ctccacccgg agcaaggcca aataggggtt cataaggtac ggcccgtact
     4681 gaacccgggt aggctgcttg agccagtgag cgattgctgg cctagatgaa tgactgtcca
     4741 cgacagaacc cggcttatcg gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg
     4801 gtttttgctt ttggaggggc agaaagatga atgactgtcc acgacactat acccaaaaga
     4861 aagcggctta tcggtcagtt tcacctgatt tacgtaaaaa cc
//
LOCUS       DAZFTN010000218         4806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFTN010000218 DAZFTN010000000
VERSION     DAZFTN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..724
                     /gene="slp"
                     /locus_tag="ACHKV2_004968"
     CDS             158..724
                     /gene="slp"
                     /locus_tag="ACHKV2_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO6942682.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            880..1410
                     /gene="dctR"
                     /locus_tag="ACHKV2_004969"
     CDS             880..1410
                     /gene="dctR"
                     /locus_tag="ACHKV2_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6942683.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1471..2499)
                     /gene="chuS"
                     /locus_tag="ACHKV2_004970"
     CDS             complement(1471..2499)
                     /gene="chuS"
                     /locus_tag="ACHKV2_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO6942684.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(2548..4530)
                     /gene="chuA"
                     /locus_tag="ACHKV2_004971"
                     /gene_synonym="shuA"
     CDS             complement(2548..4530)
                     /gene="chuA"
                     /locus_tag="ACHKV2_004971"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO6942685.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1279 a         1171 c         1086 g         1270 t
ORIGIN      
        1 tcatgccacg ccccaaaata acggaattga actaaacaga agggatataa acatcagaca
       61 ggtttacgtt actatcaggc atatcgcctc agaatcagat gaaaactata aagaaatatc
      121 tattatggtt ttaatatttg ttgataagga tagtaacatg aacatgacaa aaggtgcact
      181 catactcagc ctttcatttt tgcttgcagc atgtagttca attccgcaaa atatcaaagg
      241 aaataaccaa cctgatattc aaaaaagttt tgttgctgtt cataaccagc cggggttata
      301 tgttggtcaa caagcgcgct ttggtgggaa ggttatcaac gttatcaatg gcaaaacgga
      361 tacgttgtta gaaatcgctg tattaccgtt ggatagctat gcgaagcctg atattgaagc
      421 caactatcag ggccgactgc tcgccagaca aagcggcttc cttgatccag tgaactatcg
      481 taatcacttt gttaccatcc tcggcaccat tcagggtgaa caacctggct ttatcaataa
      541 agtcccgtat aacttcctgg aagtgaatat gcagggcatc caggtgtggc atttgagaga
      601 agtggttaat accacctata acctgtggga ttacggctat ggtgcattct ggccggaacc
      661 gggctggggt gcgccttact acaccaatgc ggtgagtcag gtaacacctg agctggtcaa
      721 ataacaccac cgaaagatgc agatgctttc tcagcatctg catcatgcat tacatcaaat
      781 taatacacag taagctaact attattatta taagccctgt cctgttaatt acctttggca
      841 aactgattat aaagttaatg tccgcaccag gagtcggtta tgtttcttat aattaccagg
      901 gatacgatgt tcttcaccgc gatgaaaaac attctgagta aaggtaatgt cgttcatata
      961 cagaacgaag aagagatcga cgtaatgttg catcagaatg ccttcgtcat tattgataca
     1021 ttaatgaata atgtatttca ttctaatttt ctcactcaaa ttgaacgatt aaaacctgtc
     1081 catgtcatta ttttctcccc ctttaatatt aaacgctgcc tggggaaagt gccggtgacc
     1141 tttgttccgc ggactatcac tatcattgat tttgtcgcac tcatcaatgg cagttactgc
     1201 tctgtgcctg aagcgaatgt gtcactttcg cgcaagcaac atcaggttct gagctgcatt
     1261 gcgaatcaaa tgacaacgga agatattctg gagaaactga aaatatcgct aaaaacgttc
     1321 tactgccata aacacaatat catgatgatc ctcaatctta agcggatcaa tgagctggta
     1381 cgccatcagc atattgatta tctggtgtga acgattgatc aatataaaga ggcccagcaa
     1441 taaatagcca gacctcccgt taattatacg ttatgcagca acgccttccg gtatcagcga
     1501 agcaatttgc ttacgccatt gcgcttgctc ctgttcgcct tctgtacgtt gaccataaag
     1561 ttgcgctatc tgcgtaccat catgggcaaa caattccaga ctggttacgt agccatcgct
     1621 ggtcggttta cgggtaaccc aggcttcagc aatgctctct tctaatagat gaagagtaaa
     1681 cgtcgggttg aaaatattca gccaaccttt cattggcacc actttttcta ccacaccggt
     1741 gaaaatctgt acgcagccac ggttgccaac aaacaccatg atttcattac catcctgctg
     1801 tgcagattca agaatttgcg ccaacgcact gttggatact ttgcaggcca aatcgtctgc
     1861 caccagattg aacgcctgtt ggcgcgtcag gttgtggcgc ttgagcaacg taaaaaactg
     1921 atgaacgtcg gtcatcgccc gccactcttg ctcgaccaca gtggcatcgg ctcgcgtttg
     1981 aacaactggc gcatcaacgg cttttaactc aagcggcgta ttctcatcgg tgataaaccg
     2041 tgccagaagc tcactccatg ccgccatatc ggtattgtcg gtggcataaa cttttagtaa
     2101 tgcatcgccc tgatgatcaa agaactgaat actctggcgt tcgccacgag ccgtgttttc
     2161 tttgatgtgg aaaacactgg cccattgatt gagaaacaga cgtaaatcca gcgcgcgcgg
     2221 attgaggatc aatccggcat gtccgttcaa atgctggttt gtgaacgtac caacttgctc
     2281 atggactgca tattcattac gacaaataca tttggtttcg ccaacacttt cgagcgccgc
     2341 cagaatttca cggatatcgc cgtgcatccg ccacgcatcg tgcgtgacgc gtgcaaatgc
     2401 cagttctgct tctctaatat tcattaaccc tgcgatgtca cgcgcgtact ttccgggatt
     2461 ttgttctttt aactcaagcc agcgtgtgta gtggttcatt gtctctcttc cttccagata
     2521 aatgccccga aatatcgggg cagatgatta ccattgataa ctcacgaaaa tttttccgtt
     2581 acgaccatcc tgtgggatgc cttgcggcga ccagtactct ttgtcgaaag cgttacccaa
     2641 caccaaagta gtggtcatac ctttgagcgc ctgttgtcct tgataactga cgtagaaatc
     2701 attcacgcca tagcctggtt gtttgctgta actgctgctg atatgtgttg agcgatcggc
     2761 aaacgtacca acccacccaa cagagaagcc actgtgagcg atcggaatat tcagagtgct
     2821 ggtaacagta tccgggttaa tgctggagat gtattcgccg gtatcggtgt ctttgccgcg
     2881 ggtacggtta taggccacat caaggctaaa cagatcagtg gtatatttcg tcatcacatc
     2941 ccagccccag attttggcgt tcgggacgtt atacgacata gtcgtcgccg ccgcgaaatc
     3001 gacggtcgtg gagatgtaat ccttcgcttt ggtatcaaag tagctggctt taaattccag
     3061 agcatcattg gacaacatca ggtcatcaaa acgcagccca aaaccgtact cctgagtttc
     3121 gttagtttcc ggacgtaagt tcgggtttgg cacccaatag ttggtataga agcgaccaat
     3181 cgagaagtgc ttagaatcgt tatacatttc gcccatcgtc ggggcgcgga atgcctgggc
     3241 atatgagcca aataacatca gccagttagt cggattgata gtcatccccg cacgagatga
     3301 ccatttgtcg gcatcaacat ctttgtaacc gtcactgcta ccgcgataac tgtcatagcg
     3361 ggttccgcca agcagggtaa tcggcagatc gcgtaaggtg atctcatcct gtagccagcc
     3421 ggagctaaaa tcgatttttg cttgcgggaa gcccgtcgtc gcgccgcccg gatgttgttc
     3481 ctgacgataa tactcaccgc catatgtcag taagtgagaa gcgaaactgt cggcaaagag
     3541 agtggaacgg ttctccagcc tggctccttt tgttatctgt tcacgatact cgccggaact
     3601 ccccgtgttt tgcgcattaa tacggacttc cgaccaataa atttttgcat ctgcatttaa
     3661 ccagtcgttg ccctgcgggg cgagtttata agaaagctgc gcatcgcgtt gaattgttga
     3721 acgatcaacc atcgggttgc tgctttcaga agcctcaacg gtctgcggat tttttggttc
     3781 acgcgcgtcg ttgttgtagt aacgcactaa accgctcaga gactgggctg aatcaatttg
     3841 ccaggtccct ttcgccagca tgttattaat ggactcgtca ttcggcgcgg tttcaccatt
     3901 gctctggcgt aaatcacccc gatcgcgact ggaccaggcc acaataccat ccagattttc
     3961 agttcgccca aacgcgctcg cgcctaatcc caggctatgg tcccccgtgc cgccagtacc
     4021 aaagacacga aaaccactgc tttgtccttc ctgcaataaa tcttttgcat cgaccgtatc
     4081 gtaggagatc actccaccca gcgcgccact gccatacagt aatgctgaag gtccacgaac
     4141 aatctcaaca cgcttgatca gcgccggatc gagaaaagtg ccattcaggt gtccggtatc
     4201 cgttccctga cgaacaccat cgacaagaac cagcacgccg cgatgatcat agccacgcat
     4261 atttacatcc tgaccgttgg ttcgtccggt accatccaga gtaattccag gaacatgacg
     4321 cagcagatcg gtggctgaag tagccgtttg attttcagga gcggaagtgt cgatgacgct
     4381 gaccatcata ggcgcttcga aggaactacg ggcattcccc gttgccgtaa cggtcatggt
     4441 ttcagtagca aaagcaaacg ttggcaaggt ggcagaaaca gctaaggcca ataaactcaa
     4501 acgcaacgag gtaaattgcg gacgtgacat agcgattctc catgaggaaa ataaaaaatg
     4561 ctggctgcct gggttaatac cggggtggct ggcgaaaaat aaatttattt aaaattcaga
     4621 gcgataatat tcgccgggtg agtttacaag cgtttatatc ccatgaaatt gcctgataac
     4681 tcggcaatta cgcaaaagca cgccctcttt gtcaggcaaa acgatatgcc ccatcaggag
     4741 tgaagagagc tgtgcgttat cccttgcaaa tgctatggtt ttgttatcca tatggtcgtg
     4801 ttttgt
//
LOCUS       DAZFTN010000219         4707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFTN010000219 DAZFTN010000000
VERSION     DAZFTN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..220
                     /locus_tag="ACHKV2_004972"
     CDS             77..220
                     /locus_tag="ACHKV2_004972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942686.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(439..1977)
                     /gene="aldB"
                     /locus_tag="ACHKV2_004973"
     CDS             complement(439..1977)
                     /gene="aldB"
                     /locus_tag="ACHKV2_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO6942687.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2085..3380)
                     /locus_tag="ACHKV2_004974"
     CDS             complement(2085..3380)
                     /locus_tag="ACHKV2_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO6942688.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3510..4661)
                     /gene="yiaY"
                     /locus_tag="ACHKV2_004975"
     CDS             complement(3510..4661)
                     /gene="yiaY"
                     /locus_tag="ACHKV2_004975"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO6942689.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1131 a         1252 c         1150 g         1174 t
ORIGIN      
        1 ttctgaagtg tgctgcgtgg ctttttaaca ggtcgaggct tatcatattg aaataatgga
       61 actatcagcc aaactaatga ataacttcag taaaacatcc acaatgcaga ttaagcgtat
      121 taaaccttca ataatctacc gtttattgct gattgtcctc ggaataccaa tggtgattta
      181 cggtctggtt tgcccgctca ccatcgaaac gcgagattaa actcctggca aaacacgccc
      241 cagaaacgca cttttgttgg cgtagtctgg gttattgcac acgtaggttt aaaacgtaaa
      301 ttgtaacaac gtgcgctttg tttatgccgg atgcggcgtg tacgccttat ccggcctacg
      361 gtcaggtccc cataggcatg ataagacgcg taagcgtcgc atcaggcaat taatacccga
      421 tgcgaccagc ttcttacatc agaacagccc caacggttta tctgagtagc tcactagcag
      481 gcacttggtt tgctggtaat gctccagcat catcttgtgg gtttcgcgac cgatacctga
      541 ttgtttgtag ccaccaaacg ccgcatgtgc cgggtaagca tgataacagt tggtccacac
      601 gcgcccagcc tgtatgccgc gccccatctt ataggccaga ttaccgttgc ggctccagac
      661 gcccgcgccc aggccatatt gcgtatcgtt cgccagctcc agcgcttctt ccatcgtttt
      721 gaaggtagtc accgccagca ccgggccaaa aatctcctcc tggaacacgc gcatattgtt
      781 ctgaccaaac agaatcgtcg gttcgaggta gtagccgtct ttcagttcac cttccagcag
      841 cttgcgccgc ccgccggtga gcacatcagc gccctctttt ttaccgatat caatgtagtt
      901 gaggatggtt tccagttgcc cgtgagaaac ctgcgcaccc atttgcgtca cgctgtcgag
      961 tgggttaccg ctgcgaatgc tttcgacacg gcggatggcg cgttccataa agcgttcgta
     1021 gattgactcc tgcactaagg cacgactcgg acaggtgcaa acttcgccct ggttaaaggc
     1081 aaacagtgca aagccttcca gcgctttatc gaaaaaggca tcttcttcat ccatcacatc
     1141 agcaaagaag atatttggcg acttaccgcc cagctccagc gtcaccggaa taatgttttg
     1201 cgtggcgtat tgcataattt gttgacccac ttccgttgag ccggtaaacg ccactttggc
     1261 gatgcgtttc gaggtcgcca gatattcgcc aatttcccca cctgcaccgt tgaccacgtt
     1321 caccacgccc ggcggcagta aatcaccgac gatttccatt agcagcagta cagaaagcgg
     1381 ggtaagacgt gcgggtttca gcaccacaca gttgcccgcc gccagcgcgg gagccatttt
     1441 ccagctcgcc atcagcagcg ggaagttcca cgggataatc tgccccacca cgcctaacgg
     1501 ttcatggaaa tgataggcca cggtttcgct atcaacttca ctgatcccac cttcctgcgc
     1561 ccgaatacac gaggcgaaat agcggaaatg gtcaatcgcc agcggtacat ccgcagcact
     1621 ggtttcgcga atgggtttgc cgttatccca ggcttcagct gtcgctaaca gctcgaggtt
     1681 ttgttccatt cgatcggcaa tcttaaacag aatcgccgcg cgatcctgca ccgaggtgtg
     1741 cgcccattta tctttcactt tgtgcgcagc atccagcgcc agatcgatgt ctcgtttgcc
     1801 cgaagacgcc acttcgcaca gcagctgccc ggtcaccggc gtcaggttct ggtaatactc
     1861 accatcggca ggggctaccc attcgccgcc aataaagttg tcatagcggt tttttaactt
     1921 gagggggaaa ccatactcgc cgggcttaat ctgtgctgaa gggggattat tggtcatgat
     1981 cgtctccttg cggtgtgagg tataacaagg gtagacgtgg ctggcgaaat cttcgccagt
     2041 cggtaacagc ttttcgacgg ctatcacgaa tttacgggca agggttacag cggataatgc
     2101 gcgaagtaca cggcttgctg gtcattaatt ttcttctctt gcgcaaaccc ttctttaaca
     2161 attgtccgta aatcagctcg aaccgtatta tcagagacgc ccaggctcgc gctaatttca
     2221 gcaactgtgt actctttccc cggattggcg agcatcacat tcaggagcgt tacctggcgc
     2281 ggattcaggc gtttaagcac accagagcta aacagcacac tatcgagttt tgcgcttcgt
     2341 tgtgtaattt catcgatatg ctgctgccag ttttgcagag cacgtttaat gacacccgcc
     2401 tgatattcaa ggaaataagt cagatccata ccatctgatt cagtgtactg gtagctggcg
     2461 gcatatttga ccggtgcagc atgaagcaaa cggctgatgg aaatatattt aaagacatcg
     2521 tagcccgatt tcagcatata ccagtaaaac agcgcccgac tggtgcgccc gttgccatcc
     2581 cgaaacggat gttcatgggc cagcataaaa tgcagaatgc aggcacggac caacggatga
     2641 atataccctt catggctgtt taaccactgg caaacttttt ccagccgttc cggtaacaat
     2701 gccgcagcgg gcggttgatg cactatattg ccatcatagt cggcgatcac aacatcatct
     2761 gtttcacgaa attctcccgg acgatatttc gcgtcattga ttccacccat accaatggca
     2821 tgtaactggc gaataagcgc aggtgtcagc ggttctgcga gtaagtgcgg aatttccgcc
     2881 atcagccggg catttcccgc aatcatatgc tcgtcttcag tgcgtggcga ccgtcctgtt
     2941 tccagcattt ttctcgccac cagcgttgta gtattcgcac cttccagctg ggagctggtg
     3001 atcggctctt ccaggcgtaa ctggcttaac tcggctccgg ccccgcgtaa ccgttcagtc
     3061 tgatctttca gcgccagcgc ggtagcacgt ttgtcgacaa gctcgcacgc ttctgcaatc
     3121 accggggtaa tgttgaatcc ggcctgttca cccgcttcat tgcaataatt aattcgttgg
     3181 atcgccgcgt tgcgcgccag acgggtaagc gtccacgcca catcgacgtt ttctcctttt
     3241 ttgatgcggt actgcaactc atcaaaaggc agataacgtc ctttttcatc ggtaacccgg
     3301 ttaaagcgca ggtgggcctg gagttcctca cccgataagt gtgataaagg aatcagcgtg
     3361 ggaggtgatt tgatagccat ttgcttatct caaaaaagcg caatattgag atagtgtata
     3421 gcaggaaaga gaaactatct caaaaaaagc aagttttgag atagttgaaa aaatggctga
     3481 caatttgcca gccgttgtgg aaatgatgat tacatcgctg cgcgataaat cgccacaatt
     3541 tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag ggcattagcc
     3601 gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc cgggatatcc
     3661 actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc cgcgtcgttt
     3721 ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc agcggcgact
     3781 ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc gtgtggcagg
     3841 ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga agcattattg
     3901 aacgccatac cggcgaggaa ctgggcataa gccattgctt cacgcgcttt cgcattactg
     3961 ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt cagtgcacaa
     4021 gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg cgttaaggca
     4081 tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga ggagtcattg
     4141 acagaaagca gcggagtgac atgtttatca acaatcgcca ttttgatatg acgcgcttcg
     4201 tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt attgatggcg
     4261 atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg aatatcgccg
     4321 ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc accgcctaag
     4381 gagatcacgc tatcgcaatt attctcttta agtaatttca aacctgcggc gacgttttcc
     4441 gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg ttcttccagt
     4501 gctttttgca catcgcccgc catacctaat ttcgttaaca tactgtcagt gacaattaag
     4561 gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga atcagcgccg
     4621 atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc ctgaaagtgt
     4681 aaaaaagggt gggcagctta ctgagga
//
LOCUS       DAZFTN010000220         4616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFTN010000220 DAZFTN010000000
VERSION     DAZFTN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            181..1413
                     /locus_tag="ACHKV2_004976"
     CDS             181..1413
                     /locus_tag="ACHKV2_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6942690.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1398..2036
                     /locus_tag="ACHKV2_004977"
     CDS             1398..2036
                     /locus_tag="ACHKV2_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO6942691.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2115..2384
                     /locus_tag="ACHKV2_004978"
     CDS             2115..2384
                     /locus_tag="ACHKV2_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6942692.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2405..3049
                     /locus_tag="ACHKV2_004979"
     CDS             2405..3049
                     /locus_tag="ACHKV2_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942693.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            3365..3704
                     /locus_tag="ACHKV2_004980"
                     /pseudo
     CDS             3365..3704
                     /locus_tag="ACHKV2_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3747..3962)
                     /locus_tag="ACHKV2_004981"
                     /pseudo
     CDS             complement(3747..3962)
                     /locus_tag="ACHKV2_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4049..4474
                     /locus_tag="ACHKV2_004982"
     CDS             4049..4474
                     /locus_tag="ACHKV2_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6942694.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            4471..4545
                     /locus_tag="ACHKV2_004983"
                     /pseudo
     CDS             4471..4545
                     /locus_tag="ACHKV2_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         1225 a         1003 c         1229 g         1159 t
ORIGIN      
        1 catggatttt ccaggtgtgt taccagtaag aagcaggtgg ataaaggatg tgataacaaa
       61 tccaacaacc aggttaatat ctgaagattt ttgttgttat tatttatccc caaagcggtg
      121 agttattaat gaagtttgcc tgaatgtata aacagaatta ttcgacataa agccaggaaa
      181 atgagtgata cgcttttaac cgaaaaaatc cttaccggag aaaatgtttt gcgtgctgca
      241 atcgcccgca ttgagtggat ttttgaaaca tttccgtcgg tatgtctgtc tttttcaggt
      301 ggaaaggatt ccaccgtgct tttccatctt gtggcagagg tggcccgcag gagaaaacgt
      361 catttctctg ttctgttcat tgactgggag gcccagtatc ggtgcaccat tgaacatatt
      421 cagaagatgc gggaaatgta ccatgatgtg acggaaacct tttactgggt ggcacttccc
      481 ctgaccacgg taaacggcgt ctcgcagttt cagccggagt ggatatgctg ggagccagga
      541 gtgacctggg ttcgccagcc accggaagag gccattacgg atatgacata ttttcccttt
      601 ttccggtatg ccatgacgtt tgaagaattt gttccggcat tttcttcctg gtttgccggt
      661 aaccggtgtg gagtggcagt gctgaccggt gttcgtgcgg atgaatccct caatcgcttt
      721 atggggctgg tgtctcagcg caaactgaga tatgccgatg ataagccctg gaccacggca
      781 tcgcctgaag ggttttatta caccatgtat ccgttgtatg actggaaagc ccgcgatatc
      841 tggatatata acgccagagc ttgtgccatt tacaatcctc tgtatgacct gatgtaccgt
      901 gccgacgtgc cgttacgcaa catgcgtgtc tgtgaacctt ttggtcctga acaacgtaag
      961 gggctgtggc tttaccatgt tctggagccg gaaacctggg ccaggatgtg tgagcgggtg
     1021 tcaggcgctg ccagcggggc gctttatgcc aatgaaagcg gtgcctattt tgccctgcgt
     1081 aaacgtatat caaaaccagc ccatcatacc tggcgcagct atgcgatgtt cctgctggat
     1141 gtgatgccgg aaagaacggc agaacattac cgtaataaaa ttgctgtcta cctgcgctgg
     1201 tatcagacgc ggggcttccc ggatgacatc ccggatgaac aggagaatga cctggggagc
     1261 cgggatattc cgtcctggcg gcgtatctgt aagacactta taaagaatga tttctggtgc
     1321 cggcccctct ccttcagtcc gaacaaaccc cggcactatg aacgttatct gcagcgtatg
     1381 aaagaaagga ggaaggaatg ggggattctg tgacaccgga gataattggc aacatgattc
     1441 gtcagtactt cagccaggaa cgaccagaag aagagaccat acaggccctt aatcacctgc
     1501 gcagagtgct tcatgaagtc agtccttttg cacatgagcc agtggactgt gtgctgtggg
     1561 taaaagcaga tgaggtggtg gccaatgatt acaatcctaa tgtcatggcg ccgggtgaga
     1621 agaaactgct gaagcagtca ctggagaaag atggtttcac acaaccggtg gtggtgtctg
     1681 aggaaaagaa ccattacctg gtggtggatg gttttcatcg ccaggtgctg ggcagggaag
     1741 caggtacggg aaaacgtctg aagggctggc tgccggtggt ctgtattaat ccggagagaa
     1801 agggacaggc ctcacgtatt gctgcgacca tccgacataa ccgggccagg ggcaaacatc
     1861 agataacctc aatgtctgat attgtcaggg acctgtcgcg cctgggctgg acgaatgaac
     1921 gcattggtac ggagctgggt atggaccagg atgaagttct cagattgaaa cagattagcg
     1981 ggctgacgga gctgtttcag gaagaggatt tcagcccggc atggacggtt cgctgagacc
     2041 gtcagatatg tatattattt cagtgagtta atgttttgag caggaaaaag agtaccccgg
     2101 aagttgtgga agacatggtt gcgcagaaac tggaggctgc tggttgctgg cgcagagctt
     2161 ctgaccgctg gctttttgtt atgggaaatg ttgaatgtac agaagcacaa cgggagtggt
     2221 tgttgttgcg ccggaattat tgtctggcgc agatatcgtc cccgccgcta cctgaaacgc
     2281 tggatatcag cgaggtggcg aaagctgcag atgccacgct gaggcgaatg gggattgcca
     2341 ctccgtccgg ggaggtattc agaaaaggta caccggtctg ttgatattca ggtaacaggt
     2401 attaatgatg aagcaaggaa tggcaaacag tatatcaatg acatggcaac ttttatgcga
     2461 tgaacatggt tttacccggg agcactacaa tcagctgaag aagttcagcc cggagacatt
     2521 acgtgaaatt attgctgaaa tagccagctg tcatccatca accagtgtat tgctcaggaa
     2581 taagtggctg acaccgccgg aggatattct ggagcagatt acccgtgaat acgaacgaag
     2641 aatacaaaac tgtccaccgt tcaggagcga gaaggaagcg gaatcctggt taaatgaact
     2701 atcttttgct gtgattgctc cgctataccg tgttgccccc gaacaaacag aacaggttga
     2761 gtcctttgtt ctgcagctta ttgccgagca ggagcgggtt tgggagctta ttttaaccaa
     2821 tgatggttat tcatggcatt gcgcacttta tgatatatta tttcatctgg ttttacgaaa
     2881 tatggaagac cagcctgaac atcagaaaca gcgaataaca aaaatatttt atgagcccga
     2941 tgcaagactg gttgcagaag agattaaatt ccggattcaa tctctgtatg atgatgaaca
     3001 aaagagagct ttaagtgaac tggtaaatga ttttacttca aaagaataaa atgcggcgag
     3061 gtgattttta ctatatgtta ataaaacggg gggtagatga aagtgaatgg tgttactaca
     3121 tcaaaatatg aaggatggcg agggacgttt cagttatata gatataaaac ggtagttctg
     3181 gtgcacccca aaggatatct ggcaggggaa atgattcatc gccaggccct aacgtcgtct
     3241 tgtcaagatt aatgctatag acatagctaa attttttatg gataaggata tgaactgtgc
     3301 ctcaggtccg gaacatcctg tagggacttt ttaagtcggg attgagctga actaatgcca
     3361 aattatgaac agggaaattc agcagcggct acagtgggta aaactctatg aaacgtccgg
     3421 tgatgcggga tttgtatgcc gtcgctgtgg catttctcgg cccacattgc gtaaaatggt
     3481 ggcgcagata tctggctcag ggcatcgctg gtctggaaag ccacagccgt cgccccaagc
     3541 gttcaccatc aacgaaaacc ggcacttgcg aagtcgctct gattctggag ttacgttctc
     3601 agcgaaatct gggggcaaga cgtattcaaa gcgagttaaa acgacttcat tcaatctcac
     3661 tggcaatagc aacaatccac aaagtccttt tccagaatca ggtttgaacc gccccgggtt
     3721 tcctggagag tgttttatct gtgaactcag gctgccagat catcgtttcc gatggaagca
     3781 taataagctt tttctgcttc tgccggagga gtatggccca gcctttccag caatcgtcga
     3841 ttgttatacc agtccaccca cgtgagtgtg gccagttcca cttctgcacg gtttttccag
     3901 ctcttacggt gtattacctc cgctttgtaa agaccattga tgctctccgc catcgcgttg
     3961 tcagtaagcg taaactgacc gccgtatgta gccatcagac gaaaattggt aacttagacg
     4021 cccatctgat atagacggac atctaagtat ggaattacag gactggcgaa aagaacctcg
     4081 taaaaactat tcgaatgaat tcaaacttcg tatggtggaa ctggcatcac aaccaggagc
     4141 ctctgttgcc cgtattgccc gggaacacga tatcaatgat aacctcctgt tcaaatggct
     4201 caggctctgg cagaacgaag ggcgtatatc acggcgtctt caggtaacga cctcttctga
     4261 cactggcgtt gaattattac ctgtagaaat aacgccggat gagcagaaag aaccggtggc
     4321 ggccattgcg ccgtctttat ccacttccac tcagaccagt gtcagtgccg gctcctgcaa
     4381 ggtggagttc cgtcacggta acatgacgct ggaaaatcca tcgccagagc tgctcacgct
     4441 gttgatccgt gaactgaccg ggaggggacg atgatctcac tcccgtcagg tacccgcatc
     4501 tggctcgttg ccgacgttac cgatatgcgt aaatccttca acggatacga catatgctga
     4561 aggaggagca gtaatggatt aagtgataac aaatacccag aaatagaggt tcaaat
//
LOCUS       DAZFTN010000221         4613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFTN010000221 DAZFTN010000000
VERSION     DAZFTN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..515
                     /gene="ftnA"
                     /locus_tag="ACHKV2_004984"
     CDS             18..515
                     /gene="ftnA"
                     /locus_tag="ACHKV2_004984"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO6942695.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(552..791)
                     /gene="yecH"
                     /locus_tag="ACHKV2_004985"
     CDS             complement(552..791)
                     /gene="yecH"
                     /locus_tag="ACHKV2_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO6942696.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            983..2194
                     /gene="tyrP"
                     /locus_tag="ACHKV2_004986"
     CDS             983..2194
                     /gene="tyrP"
                     /locus_tag="ACHKV2_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO6942697.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2256..2921)
                     /gene="yecA"
                     /locus_tag="ACHKV2_004987"
     CDS             complement(2256..2921)
                     /gene="yecA"
                     /locus_tag="ACHKV2_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO6942698.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3278..4279)
                     /locus_tag="ACHKV2_004988"
     CDS             complement(3278..4279)
                     /locus_tag="ACHKV2_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6942699.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(4285..>4613)
                     /locus_tag="ACHKV2_004989"
     CDS             complement(4285..>4613)
                     /locus_tag="ACHKV2_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO6942700.1"
                     /translation="LLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEVQLQCLDG
                     NPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSLGDVTKAA
                     FDLCDK"
BASE COUNT         1163 a         1151 c         1005 g         1294 t
ORIGIN      
        1 ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat gagcagatga
       61 acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc agctatcata
      121 ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc
      181 agcgtctgtt tgattacctg actgataccg gcaatttacc gcgtattaat accgttgaat
      241 ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat aaacacgaac
      301 aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat caggactacc
      361 caacatttaa tttcctgcag tggtatgttt ctgagcagca tgaagaagag aaactgttca
      421 aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg tattttatcg
      481 acaaagaact ctctaccctc gacacacaaa actaatgctc tccggcggca ggtttctgcc
      541 gccatttcaa atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa
      601 ggtataaatt tgccttttgc tgccagaaac gctaccagct ctcccgctgt catcccttct
      661 gccgagcaag tgtgaaaacg tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga
      721 ctggcatgcg tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga
      781 atagagtcca tcgttcctcc tcaaaaaaaa gcctagcgta gcgattgccg cttatgaaga
      841 ctttgcgcca gcgcaggact gaatgctttt tattgtacat ttatgtttac accatatgta
      901 acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat cactcataaa
      961 gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg ggaagtgttt ttatcgtggc
     1021 gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggccg gtgttggttt
     1081 tagcgttacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca cggcgctatt
     1141 actgctggag gtgtaccagc atgttccggc agataccggt ctgggcacgc tggcaaaacg
     1201 ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct taatgtatgc
     1261 tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca tcagcgactg
     1321 gacaggtatt tcaatgtcgg caaccgctgg cgtgttgttg ttcacttttg ttgccggtgg
     1381 cgtggtttgt gtcgggacat cgctggtcga tttatttaac cgttttctgt tcagcgccaa
     1441 aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca aagtgaatct
     1501 tttaaccctg ccgttgcaac aggggctggc actgtctgca atcccggtga tttttacgtc
     1561 gtttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca acattcgtaa
     1621 gctacgctgg gtgtttataa ccggtagtgc gatccccctg gtggcatata ttttctggca
     1681 ggtggcgacg cttggcagca ttgattcaac aacctttatg ggattgctgg ctaatcatgc
     1741 cggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc atgttgagct
     1801 ggcagtgcat ttatttgctg atttagccct tgccacgtca tttctcggcg ttgcgttagg
     1861 cttatttgat tatctggcag atttgtttca gcgttcaaat accgttggtg gacgattgca
     1921 aactggagca attacctttc tgccaccgtt ggcgtttgca ctgttttatc cacgaggatt
     1981 tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga ttatcccttc
     2041 gctgttgacc tggcaaagca gaaagcacaa tcctcaagcg ggttaccggg tcaaaggtgg
     2101 tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg tgcaattttt
     2161 gattgcggca gggttgttac cagaagtggg gtgattagat agcctcaaat tccttattgg
     2221 gtgccagaat taacgctgac acccaatttg acttcttaat gcaggcagca ctgcttaaat
     2281 tttttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc tgctttgatc
     2341 ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag atcaagcgcc
     2401 gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatctt ctctacccgc
     2461 tcgaagtttt cctcagtacc gtgcagcgct atcgcctcca gcgctggttt taacgaatcg
     2521 ggcaatgttg accagtcaga aagtgccacg ccccgcatat agccaaagca ccactcctca
     2581 acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc aaactgctcc
     2641 gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag gttcataaag
     2701 cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc ccacacggca
     2761 accagccact gtgccggttc aatctcttgc ggagaactca acaccgccgt caataaaccg
     2821 tccagctccg ccacatcaag gatggcgtgg tcagtgttgt atttggtcag aatatcgtcc
     2881 agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc cttgtggatc
     2941 tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt ttgagtggct
     3001 ttgccggtga ttaaaaatca aggagggtgt aacgacaagt tgcaggcaca aaaaaaccac
     3061 ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct ttcccatggt
     3121 acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa aaactatcaa
     3181 ccaccattta taaatcataa ccttatgatt ttattaactt tgaaaatggc tctatacaac
     3241 gccatttgaa tctattgtca ctttttatcg ccacttttta ccctctattg tcaaaagggt
     3301 tcaattcaac agcagcctct aaatgacctg gagcaaaatg cgcataacgc atagtcattt
     3361 ttatatcgct atgccccagt attttttgca acacaagaat atttccaccc cgcatcataa
     3421 aatgactggc aaacgtgtga cgtagcacat gagttaattg cccatcagga agctcgatct
     3481 tcgctctctt aattgcagcg tcaaaagcct cataacatgg tgaaaatagc gctcctcgtt
     3541 ttttaggaag catagcttgc aattgaggtg aaatcggtac agtgcggttc ttctttcctt
     3601 tagttttaac aaatgtgatt cgaccgggca gtacttgaga ttgctttaat ccttctgctt
     3661 cactccaccg agcaccagtc gcaagcccaa tacggacaac aacccccaaa tctttattcc
     3721 gtgactcatc acacgcaatc agaaggcgtt caatctcatc tacatacaga aacgccagtt
     3781 ccttttcttc ctcacgaaac ttgcgaatac cagtcagggg gttttcacca gaccactccc
     3841 caaggcgctt cagttcggca aaaacagcat gtagatatga ctgctcgcga ttaacggttg
     3901 cttcactaag ttttttcttc cccttgggat tccattctcc tgatagcctt ctttcccgat
     3961 aagtagcaaa catatttttg tcaaactgag aagcaaatgg atctcccagc ctggaacaaa
     4021 tcgcctcaag tttgactttg cgctctgcac cagaggacaa ggttttaccg tacatctcaa
     4081 accaacgagc aatcaactca gaaagacgag gaccagaacc atcttgaaac tcgtctccaa
     4141 ctctactatt cattaaacgg cgctcataag agagcgcctc actttttgtc gcaaactgtt
     4201 tacgaatgcg ttttcccgat gccccgtagg gataacattc acaaagccat ttacctgatg
     4261 gaatcttacg aaccgacatt ttagttactt atcacataaa tcaaatgcag ccttagtgac
     4321 atcccccaga ctctttttta accctggggc ggcatcatta tctagccaaa atggattatt
     4381 gttatctaac ggtaacgcac caaatgtttt accttttatt cgagccaaac ctgtaagtgc
     4441 atataactta ttatcgtcaa aattcatcac ataaggatta ccatcaagac actgtaattg
     4501 aacctcatca gtattaaatg gccatacccc attgaaactc tcacgttcaa tagttttaaa
     4561 aggcattgcg acggcggaaa aagaaaacat agataaaaaa gtaactaata gtt
//
LOCUS       DAZFTN010000222         4596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFTN010000222 DAZFTN010000000
VERSION     DAZFTN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..805
                     /locus_tag="ACHKV2_004990"
     CDS             <1..805
                     /locus_tag="ACHKV2_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO6942701.1"
                     /translation="AKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTD
                     EELNDREFIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFY
                     EESKDNEPLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGR
                     VNINEYNDNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLA
                     PDILSKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
     gene            1167..1271
                     /locus_tag="ACHKV2_004991"
                     /pseudo
     CDS             1167..1271
                     /locus_tag="ACHKV2_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            1418..1951
                     /locus_tag="ACHKV2_004992"
     CDS             1418..1951
                     /locus_tag="ACHKV2_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942702.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            2148..2321
                     /locus_tag="ACHKV2_004993"
     CDS             2148..2321
                     /locus_tag="ACHKV2_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942703.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(2369..2650)
                     /locus_tag="ACHKV2_004994"
     CDS             complement(2369..2650)
                     /locus_tag="ACHKV2_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFO6942704.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(2687..3037)
                     /locus_tag="ACHKV2_004995"
                     /pseudo
     CDS             complement(2687..3037)
                     /locus_tag="ACHKV2_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(2995..3192)
                     /locus_tag="ACHKV2_004996"
     CDS             complement(2995..3192)
                     /locus_tag="ACHKV2_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942705.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            complement(3339..3812)
                     /locus_tag="ACHKV2_004997"
                     /pseudo
     CDS             complement(3339..3812)
                     /locus_tag="ACHKV2_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(3809..4159)
                     /locus_tag="ACHKV2_004998"
     CDS             complement(3809..4159)
                     /locus_tag="ACHKV2_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFO6942706.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            complement(4150..>4596)
                     /gene="yfdR"
                     /locus_tag="ACHKV2_004999"
     CDS             complement(4150..>4596)
                     /gene="yfdR"
                     /locus_tag="ACHKV2_004999"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6942707.1"
                     /translation="LSNICRFAGHLSHFYSVAQHAVLCSQLVPQEFAFEALMHDATEA
                     YCQDIPAPLKRLLPDYKRMEEKIDAVIREKYGLPPVMSTPVKYADLIMLATERRDLGL
                     DDGSFWPVLEGIPATEMFNVIPLAPSHAYGMFMERFNELSELHKCA"
BASE COUNT         1270 a          936 c         1007 g         1383 t
ORIGIN      
        1 tgcaaaaata gatttattta ttcaaagaat cactcataat ttatggactg cacgtgaaaa
       61 aaatgtgacg ttaattaagc aaataaacga tctcaaaatg tgtgtcaatg agtatattgg
      121 agattgtact gatgaggaat taaacgatcg tgaatttatt gcatcagttg ttgacagggc
      181 tatttttcat ttcgcgatta atagtatatg taatcccgag gataataaag atgcgacact
      241 catagaacga tgtacttttg atgtagaaac caagaatggt cttccctcca cggttcagct
      301 attttatgag gaatctaaag ataatgaacc tttagcgaat atacatttgc aagcaatagg
      361 ttctggtttc ttaacgtttg ttaatgcctg ccaggaacat gatgacaaca gcttaaaatt
      421 atttgcttcg ctgttaattt ccctttcata ttctagtgtc tactcagatt ttgcaggaag
      481 agtgaatatt aatgaatata atgataacta cctgaaagct cagtttgaag aattatctca
      541 acgtgatatg aagaagtacc tgggagagat gaagcgtctg gcagatgggg gagaaatgaa
      601 ttttgatggc tatctggata agatgtcaca tctggtgaat gaaggaacgc tcgcccctga
      661 tattttaagc aaaatgcgag atgctgcacc gaaattaatt gactttgcta agtcgtttga
      721 cccaaactca aaggaaaaga ttaaaatata tacaggcact tctaaattaa tttatgattt
      781 attcggggtt aaatcggaga aataatatgt gaagttcttc gatggtatga aaggtattac
      841 ataaaagaac ccaatgttta ttgggttctt tttttctcta tcagtgctat tagcagggag
      901 agatatcacc ggagtttaat gtgtgatttt tatttatcgt cgaatctgta ttgtttatca
      961 ttgcttttca caacgctaac atctaataag attagatatt cacttcgtga gagcttcatg
     1021 ccttgagagg atctcaattt tcttttgcaa tgagacaggc gcttcctgtt gttatggtat
     1081 agtaccccgt tattgagcct cctgaacagt gatgctgaat aacataacca catgatatat
     1141 cgataaaata atctctacat ttgaaaatgc acggtaattc tgaaatgcaa aaaatcaacc
     1201 aaaccagcgc aatgcctgaa aaaactgacg ttcactggag tggtcggttt agcgttgcac
     1261 caatgctcga taggatgtac cgtttttgaa aaacaaatag ttatatactt tgtgggagcc
     1321 tattgggaac ccggcgtttt catttcaagg tgtaatccat acgcggctta agaatgagat
     1381 ataatgcgac ttttagtgtt ccgcttgaga ggccatgatg cttaccctgg acgagatagg
     1441 tcaatcagta cgtaacaata tccagttgat tattgatcat gtcggcttac ctcttgctgt
     1501 tggtccgctc agtgatgatg attacaagat tctgtgtggt ggctatggtg agcttgaatg
     1561 ggactatgcg ttaagtacct atggcaactc cagagaaaag tatgagttct gcataaaact
     1621 tgttcagcaa ggtcgggttc agggaatacc atcaggagca gcaatttgtg tttatggggt
     1681 tgaagaaaac atctttcgta tccatatgat cgaaaggttt tctagagaag atgaatctca
     1741 cccattgaaa gggcgcatgg ttttactcac tcttatgagt gcttttatat tttgtaaagc
     1801 tgttgaatgt aaagttgtcc acattgtaga gccagtacca gaactggtgc agtattacga
     1861 gtcttttggt ttccgcatgg aacagtgcgg ttatgtgatg tcggcagtca ttgatgagct
     1921 gcaggatatc tttcttaaat ttgctcagta ggtatagacg agaagggtct acaaattgta
     1981 ggatacccgt ccagattacc ttaaaggtac atctatggca gtcgttttgt gcttaaacta
     2041 ctaagaaacg atgtcaccaa tcgacatgat cgattggcat aagttagcga aacaagctag
     2101 ctttaaagaa agggttagag acgcctttac tgtctcggga gttttctatg aaagatcaaa
     2161 aagcaaccaa gccacaggtt aagttcgaca caatgaaagc attcgcaggt atgggtgctg
     2221 ctgttgaagt tctgatgaag gctgctccta atgcgttcac tcacgctact gtctctggta
     2281 aagagcagca gggtaagctt cgtcgtcgca aagcagcatg atcatagctg gtgctttttg
     2341 aaaacccgcc ttcaggcggg ttttttcttt agtgatgttc tttgtccttc tgtttgactg
     2401 ttctgacctg ttcccactcg atacgtcctt cttctcgcct tttgtctatg tattccgcaa
     2461 gatcttgaat attgatgcaa cgttttgctt tttgtgatgt gccgatgcga tatgttggaa
     2521 cgggcaactt acaagcgttt gcttttgctt ctgccgtggc tggactcata ccaaagtact
     2581 tttggctaac tgctgagagt tcaatgtttg gggtattgaa ttcagccatc agtaaaaaca
     2641 aggtgttcat aattttctcc atcaaaaccg gctgcacccg ggaaaatcat aattctgtgc
     2701 tggtggcagg aattaatttc tgccagatag cggaaacata ttttgcctga tgacgggcat
     2761 cagccagggc gttgtgccgt tcgccatcga aaggcatgtc cattttgggg tcgaatccga
     2821 tggaacgccc aagcgtaacg atcgtgcgta catcgtggtc attccagtac gcccacgggc
     2881 agatttgtcc tgctcgctcg taagctccac gtaaaattac gttgtcgaag gtggccccgt
     2941 taccccagac ttttaaatat ttcgtattgt ctgcgtgccg gttaatgaaa tggtttagtt
     3001 ctgagagagc atcgctgatc gacaaagtat catcaatact tccagcgata ccagcgcgac
     3061 tttaaatgcg gtaagtacgt ttttaaccac atcgattttg aatactattt catcacatac
     3121 aaacgattta tcgtctacta ccgtttcaat tccggtaatc gtgttttgta gccatttggt
     3181 taattcagcc atttttcatt accgcccttt cgggcggtct cctgatgttc tgagggtgca
     3241 ggaatccctc cggttaagga ttaaatttta tttacagaac tgaatttaat tattcagata
     3301 tacgtatctg taaccttacg aacctactca ctggatgcct atttcataaa aataatccag
     3361 tgggttttat cgttttttcc tgttcgttga ccgataacag gttttctgtc ggtcagtacc
     3421 aatatctggc gaacaggtat ttgcgtttca ttccatttaa aaatcagaac gccggatgga
     3481 cgcaacacac gaaaggcttc tttaaatccc tgccgcaaat catcacgcta ggtatcttta
     3541 ttcagccgtc catatttctt tcccatccag gcgttatcac caacacgctc aagatgcgga
     3601 gggtcgaata taaccatcga aaaagatgcg tctgcaaatg gtagtgcacg aaaatcagct
     3661 atcagatcag gactgataat caggcgtcgt ccatcacaca atgtgtgctc ttcctttctg
     3721 atatcgctaa atatcgcccg gtcgtcattc ttatcgaacc agaacatgcg actgccacag
     3781 cacatgtcga ggattgctgc atgtccagtc actggctgcc tcctttgcga agccgttccg
     3841 cccattcttc aagggatttc tctgcatatt caccggacag gccatcaatc ggatgcgatt
     3901 cattagctaa ctcttctttt gctgacagaa tcatgcgtgt aacgtcgaaa acttcagcca
     3961 aaggcttatt gataaatccg tgattgaaag cagcagcaag acggcttgcg gtatagttaa
     4021 taccctcgtt gcgagcctca gcacgtactt catcgaattt acgcaccaga tactcagcat
     4081 ttgtttcatt cactttcaga tctcgcggta cacatttccc gcgaagaaac ccttccattt
     4141 cgaaaacatt catgcgcatt tgtgtaactc cgataactcg ttaaaacgtt ccataaacat
     4201 cccgtaggca tggctaggtg ccagtggaat cacgttgaac atctctgttg ccgggatgcc
     4261 ttccagtaca ggccagaaag agccatcatc aagcccgaga tcgcggcgtt cggttgccag
     4321 catgataaga tcggcatatt tcacgggcgt actcataact gggggtaacc cgtatttctc
     4381 acggattacg gcgtcaattt tttcttccat ccgtttatag tcaggaagaa ggcgtttcag
     4441 tggagcgggg atgtcctggc aatacgcttc tgttgcatca tgcattaatg cttcaaaagc
     4501 aaattcctgc ggtaccagct ggctgcaaag aaccgcatgt tgggcgacgc tgtagaagtg
     4561 cgaaagatga ccggcaaagc gacagatatt tgaaag
//
LOCUS       DAZFTN010000223         4567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFTN010000223 DAZFTN010000000
VERSION     DAZFTN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(50..448)
                     /locus_tag="ACHKV2_005000"
     CDS             complement(50..448)
                     /locus_tag="ACHKV2_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6942708.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(490..1515)
                     /locus_tag="ACHKV2_005001"
     CDS             complement(490..1515)
                     /locus_tag="ACHKV2_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6942709.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1571..1903)
                     /locus_tag="ACHKV2_005002"
     CDS             complement(1571..1903)
                     /locus_tag="ACHKV2_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6942710.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1913..3232)
                     /locus_tag="ACHKV2_005003"
     CDS             complement(1913..3232)
                     /locus_tag="ACHKV2_005003"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6942711.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(3213..>4567)
                     /locus_tag="ACHKV2_005004"
     CDS             complement(3213..>4567)
                     /locus_tag="ACHKV2_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6942712.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDG
                     QTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQ
                     EQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLL
                     RYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLE
                     EAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTY
                     EKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA
                     "
BASE COUNT          912 a         1424 c         1242 g          989 t
ORIGIN      
        1 gcaatggcag catcgaacag gttatcgaaa tcagccacag cgcctcccgt tattgcattc
       61 tggccaggcc gcgctctgtc atttcggctg ccacaccggc agagacacga aacgccgttc
      121 ccggcagcac aaatgccaca ggttcatccc gcgtgacgtg aagtgcatca gtatgcagct
      181 tcaccagtgc cacgaccgtg accagttcag acgtatccag aatcacggta tccggctgcg
      241 ctgatcccac ctcattttca tgtccggtca gcacattttc ccggctgaga ggggtgtcct
      301 gaccggcagt ttcatccgtg tcatcaagct cctcttccag ctctgccaca cggagcgcca
      361 gttcttcttt cgtccccgtc aggctgacat cacggttcag ttgttcaccc agcgagcgga
      421 gacgggcaat cagttcatct ttcgtcatgg actcctccac agaaaaacaa tggccccgaa
      481 gggccatgat tacgccagtt gtacggacac gaactcatca gggtcagcca gcagcatcag
      541 cggtgctgac tgaatcatgg tgaactcacg cgccggatcg ccggtggtca cccagttttt
      601 cgggtaacgg gcagaggcgt taatgccttc gcgctgtgcg tccgcatcct gaatgcagcc
      661 ataggtgcgc agaccgcgtg cctgagtgtt ccccagcacc atcgtgttgt ccggcaggaa
      721 gttctttttg acgccgtttt ccacgtactg tccggaatac acgacgatgg ccacatcgcc
      781 atacattccc ttataagaca ccgctttgcc caggtctttt accgctgtct ccagttcgga
      841 atgagagccg cgacgggtat ccagcttctc cctgacggct ttgaaggaac ggaacagcgc
      901 ccagcctttc gggtcaaaca cgatgatatt caccacgccg ctggcgttca gcgcgtaggc
      961 ttcgatatcg tcggtcgggt catacgtgga cttgtcacgc ttgctccact ccgtgccgcc
     1021 ggactgcgtg atgttgttcg ccgcactgcg gcccatatcc acctcaaccg gatcgaaggc
     1081 ttcaccggtc atggtgtatt tgcccttgag cacggcagaa actgcctgca tctcttcgac
     1141 ctgagcaatg gccagctctt cgtcacgcat gttctgcatg atgatgcgac ggcggcggta
     1201 agccgggtcc gccagattct gcggatcttc atccggcagg cgacgcaggg tcatctgcgg
     1261 attcacttca tgcttcggct tgacatatcc cggcgtaaat tcagaggtgg agccgccacg
     1321 ggaacggata acctcaccag aaacaatcgg cgaaacgtac agcgccatgt ttaccagtcc
     1381 cggaatttgt gagagataga ctttctccgt ggtgaaggga tagctctcac ggaaaaagag
     1441 acgcagaaac agcggatcaa acttaaattt ctgctcattt gccgccagca gttgggcggt
     1501 tgtgtacatc gacataaaaa aatcccgtaa aaaaagccgc acaggcggcc tttagtgatg
     1561 aagggtcagg ttaaacgatg ctgattgccg ttccggcaaa cgcggtccgt tttttcgtct
     1621 cgtcgctggc agcctccggc cagagcacat cctcataacg gaacgtgccg gacttgtaga
     1681 acgtcagcgt ggtgctggtc tggtcagcag caaccgcaag aatgccaacg gcagcaccgt
     1741 cggtggtgcc atcccacgca accagcttac ggctggaggt gtccagcatc agcggggtca
     1801 ttgcaggcgc tttcgcactc aatccgccgg gcgcggttgc ggtatgagcc gggtcactgt
     1861 tgcccagcgg ctggtaatgg gtaaaggttt ctttgctcgt cataaacatc ccttacactg
     1921 gtgtgttcag caaatcgtta acggcatcag atgccgggtt acctgcagcc agcggtgccg
     1981 gtgccccctg catcagacga tccagcgcag tatcactgcg cgcctgtgca ctctgtggtg
     2041 ctgcggccag aatgcggcgg gccgtttcca cggtcatacc gggggtttct gccagcacgc
     2101 gggcctgttc ttcgcgtccg tgagcctcct cacagttgag gatccccata atgcggctgt
     2161 tttctgccgc aaccgcagcg gtgatctgcg cgttcacgtc cggctgcgcc gcgctggcgt
     2221 tttcgccctc cgtcgctggc accacgccag taacgtcagc ctgcgaagca gtggctgaaa
     2281 cagttgttga ttgagtctct ttggtcattc gccctcctga gagacgggat ttacgtgcat
     2341 ccagtgcatc acgcataacg gtgatcgcat cggtgctgtt gacaagttca tcagccagtc
     2401 cggcatcaat ggcctcctga ccgctgtaca ctgcagcctc ggtatccagc acagcctgca
     2461 cggacaggcc ggtatatgcc gacaccttct gcgcaaacat ccggcgggtt gcatccatcc
     2521 gggactgcag tgtttcccgg acatcacccg gtagatggct gtaggggttg ccatccacct
     2581 tatggctgcc gctgtaaatc agcgtgattt ccacgccctg tttctccagg gcagcaccgt
     2641 aattactgtg agccatcatg acgccgatgg agcctgtccg ggcggtctgc gtgaccagac
     2701 gccgggaggc ggcgctggca agcagctgac ctgcactgca gttcatgtcg ttggccagcg
     2761 cccataccgg ttttatgtct cgcacacggg cgatgatgtc agcacagtca aatgctcccg
     2821 ccaccatccc gcccggtgtg tccatatcga gcagaatgcc gtccaccatc ggatcgctgg
     2881 cagcctgttg cagacgggcg ataatgccgt tgtaaccggt cattcccgaa tacggctgca
     2941 gcgcccgcgt ccggctgacc agcgtaccgg acaccggcag cacggcgatg ccgttcatga
     3001 cctgataact ccgggcctgt cgtggtccgt catcatcacc ggataacgcc agcgccgcgg
     3061 gtgcctctcc ggcagtcagg ctgtcgccgg atactgcatc cgtcaggcgg ctgatcccaa
     3121 gctggcctgc aagcgcacaa aagaaaaccc gcgcataggc gggttcaagc atcagcggct
     3181 cattaaaagc catgctggca atatgcggga gattacgcag ctctgctgtc actcttctcc
     3241 tcctctgttg attgtcgcag cccggattca aatgccgcag ccgcccaggc gggcggttta
     3301 agaccagctg cgcggcgctc catcgtttca cggacctgct gggcaaaaat ttcctgatag
     3361 tcgtcaccgc gtttcgcgca ctctttctcg taggtgctca gtccggcttc tatcagcatc
     3421 accgcttcct gaacttcttt cagaccatcg atggccatac gaccggagcc tatccagtcg
     3481 cagttccccc aggcactgcg ggcttcctga aagctgaagc gcgcttttga aggtaacgtc
     3541 accacgcggc gaacgatggc ctcttccagc cagcacagaa acatctggct cgcctgacgg
     3601 gatgcgacga attttcgccg ccccataaag tacgcccacg actcgttcgc actggcccgt
     3661 gccgtggagt agctcatctg ggcgtaattc cgggaaagct gctcatacga gacacccagc
     3721 ccggcagcga tataccgcag cagtgactgc tcaaacacgg agtagccgtt atccgtgtcc
     3781 tgagccgtct gcaggttcag tgagtccccc ggcatcaggt gcggcacttt tgcgcctccc
     3841 agacggaccg gtgctgcggc gtaatacgcg gcaatttcac caatccagcc ggtcagcctt
     3901 tcccgctgct cctgactgtt cgcgcccaga ataaaatcca tcgctgactg cgtatccagc
     3961 tcactctcaa tggtggcggc atacatcgcc ttcacaatgg cgctctgcag ctgcgtgttc
     4021 tgcagcgtgt cgagcatctt catctgctcc atcacgctgt aaaacacatt tgcaccgcgg
     4081 gtctgcccgt cctccacggg ttcaaagacg tgaatgaacg aagcacgacc gccgggtaac
     4141 tcgcggggta tccatgtcca tttctgcggc atccagccag gatacccgtc ctcgctgacg
     4201 taatatccca gcgccgcacc gctgtcatta atctgcacac cggcacggca gttccggctg
     4261 tcgctggtat tgttcgggtt gctgatgcgc ttcgggctga ccatccggaa ctgtgtccgg
     4321 aacagtcgcg agggacgggt atcccaggtg gcctgaacga acagttcacc gttaaaggcg
     4381 tgcatggcca caccttcccg aatcatcatg gtaaacgtgc gttttcgctc aacgtcaatg
     4441 cagcaacagt catcttcggc aaactctttc catgccgctt caacctcgcg ggaaaaggca
     4501 cgggcttctt cctccccgat gcccagatag cgccagcttg ggcgatgact gagccggaaa
     4561 aaagacc
//
LOCUS       DAZFTN010000224         4541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFTN010000224 DAZFTN010000000
VERSION     DAZFTN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..230
                     /locus_tag="ACHKV2_005005"
                     /pseudo
     CDS             129..230
                     /locus_tag="ACHKV2_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(640..875)
                     /locus_tag="ACHKV2_005006"
                     /pseudo
     CDS             complement(640..875)
                     /locus_tag="ACHKV2_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1034..1684)
                     /locus_tag="ACHKV2_005007"
     CDS             complement(1034..1684)
                     /locus_tag="ACHKV2_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6942713.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            1916..2972
                     /locus_tag="ACHKV2_005008"
                     /pseudo
     CDS             1916..2972
                     /locus_tag="ACHKV2_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3191..3781)
                     /gene="espM1"
                     /locus_tag="ACHKV2_005009"
     CDS             complement(3191..3781)
                     /gene="espM1"
                     /locus_tag="ACHKV2_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO6942714.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            3965..>4541
                     /gene="nleG"
                     /locus_tag="ACHKV2_005010"
     CDS             3965..>4541
                     /gene="nleG"
                     /locus_tag="ACHKV2_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO6942715.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKD"
BASE COUNT         1367 a         1041 c          845 g         1288 t
ORIGIN      
        1 tgttgaactc tttttttgtt ttgcaggagt tactcctctt accttattgt ccactcgatg
       61 accaacacat tcacaaccca aaccagaata tcctatctat gaagcatgta tccgcacgca
      121 gattaattat ggtttacatt gttctaccac aatagtcgca tcctaaatgg gtgcggttga
      181 ggatcaccat ccttgaagag gatgcccgcc actttaactg catgacaggg ctatttaaac
      241 aatcactaaa aatcgcccaa gagcattctg tttctataat gtattccaag agattaaaaa
      301 ataatatcat acgttattcg gcagagttcg tatgtactac ggcgtcccaa ccatgtccca
      361 aaacaaaaat ttctgcccta tacacgttcc aaaaaattca acagcaaaga gagtctgctg
      421 acgagttgtt gattcactga ttaatacgta aaattgataa ctgctggaaa tcatacaata
      481 ctcgtacttt cgaaagttcg ctaaccaggt gctacacgcg aggtgttgca gttttcttta
      541 cgatttatca ttcaatccta atccatgcat gacatgtggt gcctgtgggg tgatcctacc
      601 tacgtaatgt ggacacagcc ctaatcaagg ttctgttttt caaaacagtt ccgtgctgag
      661 acagccacaa gtactgtgtc ggcaccaccg attgtaatca cactagatct aattaaacac
      721 agccgtctgc attatttccc gactcataaa atattagccg taaatgcatt cgacttttag
      781 tgagtggaag aaactttccg ccctagcatt atcgtaacag cgcccctttg cactcatcct
      841 gtcatgcagg tatgccgctt cagaagcgcc tgataatgcg ttgaaatcag gacggtggct
      901 acttgctatt cagttcacga tacatccctt aaggaccagc gaatagcaat gagtcacttt
      961 attatcaagt ttgatgaccg ctttgatgct aacttttaat aagagtctct tcacacaaaa
     1021 tcgtgtaccg agtctacact gaataacaat cactcctcac cgtaaagtga ccgatactgc
     1081 aatcaaactc acactgctct ctggccacaa gcattgataa tgttattggc tctctgctta
     1141 gtgggtgatg catcccatca ttaattatac gagaaagagc catttcatca tataaactac
     1201 atactctgga accttcagca tttcttaaaa aaacacctcg ttgaggaatt gccagcgtaa
     1261 tcgggcactg taatgcttca tctatgtaag acatttcttc aatattaact aaaaatgaac
     1321 atgaatgtaa tcgccgctgt aacaattcaa ttctgttatc attactatgc ataaattcag
     1381 aaataacctg ccccatatat gtactgaata catcaagaaa tgaattgcct ccattcaatt
     1441 gattttcaac agaatcagcc aatctaatga tatcggctag ttctgtatgg cttaaacccg
     1501 aatatagttc cccttgaaca ggatctacaa taaaagcgtc catagcactc tgatagcgaa
     1561 cagaaaacga tctttctcct attgtgagtc tttccatttg atgatctctg gtcgcacgtt
     1621 gtaaaacttc aacatcttgt gttgacaagt aatttccaga agacaaatta aatgaaaatg
     1681 gcataatttt atccctaaag catatatctt acttttcgat aattaacaaa aagccatcct
     1741 ccacagcaat actccatcag aataattgta ggattatttt aatctaattt ataagcataa
     1801 aatatatact cactaaatag ctgcgcagaa tagtagatca tttagaggga actcaacccg
     1861 attgtgcgat ctgatcaatc gccaaatcaa tacaaatcac caaccggact gagcaatgtc
     1921 gatcatacca ccaatttccc gtgacgaacg acgcctaata cagaaagcca tccataaaag
     1981 gctgacagcc atgctgatgc tgcaccgggg cgaccgtgtc agcgacgttg cccgcacact
     2041 atgctgcgcc cgttcctctg ttgggcgctg gattaactag ttcacacagt cgggtgttga
     2101 gggactgaaa tcattacctg ccgagcgagc ccgccgctgg ccgtttgcgc atatctacac
     2161 actattacgt gaacaggtaa aatattcact cgcgactttg gctacaatta ttcacgctgg
     2221 agtacagaac tgctgacaat aaaaatcaat gagataaccg gctgcctgtt acatgctgga
     2281 accattcgcc gctggttgtc gtctgtgggg cttgtgtggc gaagggctgc gccaactcta
     2341 cgtatccgtg ccccgcctaa agataaaaag atggcaacaa tccataacgc actggacgaa
     2401 tgcagcacag agcatccggt cttttatgaa gatgaagtat ttatccatct taatcccaaa
     2461 atcggtgcgg actggaaact gctcgggaag caaaaacgag gggtcacgcc ggaacaaaat
     2521 gaaaaatatt ctctggacgt agcgctgcac agcgggacag gttaagtcag ctgtgtgggc
     2581 gccaacagca aaagttcggc gctgttcatc agactgctga agcggcttaa agctacatac
     2641 tgtcggacga aaaccatcac gctgctcgag gacaactaca ttatccacaa aggccgggaa
     2701 acccagcgct ggctgaagga taaaccgaag tgcagagtca tttatcagcc ggtttaatct
     2761 ccatgggtga atcattttga atagctatgg cagatacttc acgccacaat aacgcgtaat
     2821 catcagtgcc gctcaatgtg gcaactattg aaaaagttcg ccattttatg gaatccgtca
     2881 gtccattccc cgaaggcaaa catgggctgg caaaaatgtg cgatattagg cgcagctatt
     2941 tagttattcc gtcatttctc ccgcccgtgt aaacactact gaatcttgtc gttgtgacaa
     3001 agctataaat agtcaatcac agcacgtttc tgcgtacgac gcgctgcaat tttctttacg
     3061 atttataact gtacaggtga actatgagtt atttggatac atacatatag tgtaacgcct
     3121 gttgtatatt ttactcatat ctttttacag gatgaaaacc aatgatactg gcgcatagcc
     3181 tcctccaacc ttacccctgt ataacacgac tcatataacc tgggatttca tttcgtagtt
     3241 ttacaatatc aacacccggg gcgatatgtt tctgataaaa cttgtctgca agcacgtttg
     3301 ctatgtaatc tgttgtactg ttttttacag ctcggctcat accatcgcaa agagcatcca
     3361 tttttttgcc aaaagaagca tttcctatta ttatatgatt aatagaactt tgtgccgctt
     3421 taacatcaag agttagttca aactccttac ttacaagaga aaagaccatt ttcctctctt
     3481 cagaagaaat ttttgcattt ttttgaaggc agcaattatc aatctcttgg ttgataaccc
     3541 ttgacggata tactgtccgt tcatcactta cccatccatg aatatcttta gtactctgcc
     3601 tgcttaccgt ctccagtata cagccgctaa atttgttaac attaaagcgg atagagcgct
     3661 ctcccattga acacttcacg acctcatcat tcttcccacg gatggtgccc cggaaagaat
     3721 tatctttatg ataactgata ccaaaagagc tgaaggacac acctgtcgca tttactggca
     3781 taacaaatcc ttttcattaa aatttgctta caaaaattgt atacaatcat tttaattaaa
     3841 aaaatacatg tgtaaatagc attcgttcaa aatttaattt tttgtttgaa tcctatcaca
     3901 tatttagtat cttgactcct gtcaccaata tggttcaata acatagacta aagagggaga
     3961 aaatatgcct gtcatattaa acttttcgag tgaaagagta ttgtctgaaa gtgaactgga
     4021 ggctttacgt catgtcggaa gagttagtca gagtgaacag cttgttgtga gaggccgcac
     4081 tatgagactt catcatattt catttatgga cagttttagc gtggaaccgg tttccggcgg
     4141 actgttggat cgccttagtg caagagggca tcgccttctc gcagaaaatc tggagataca
     4201 gcttaacaga ggccatactt ttttgcaggc attccgttta tatatggaac agagccgtgc
     4261 aaccccatgt acgcgccaaa acgtcagtag tgcaatacaa aacaaaatta actctcatgc
     4321 atttactgtt agtcatcaag acttttcctg tcatgaacaa cacctgaatt gcccgataac
     4381 attatgcatt cctgaaactg gtgtttttgt cagaaatgca aaaaactcag aaatatgttc
     4441 attatatgac cacaatgcac tcactgaact tatccgacgc aacgctccac accctctcag
     4501 ccgtgaaccc tttgttccgg aaatgattgt cagcaaagat g
//
LOCUS       DAZFTN010000225         4449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFTN010000225 DAZFTN010000000
VERSION     DAZFTN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..149
                     /gene="safA"
                     /locus_tag="ACHKV2_005011"
     CDS             <1..149
                     /gene="safA"
                     /locus_tag="ACHKV2_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO6942716.1"
                     /translation="EIMSVIVVILLLTLTLIAIFSAIDQLGISEMGRIARDLTHFIVN
                     SLQD"
     gene            223..984
                     /gene="ydeO"
                     /locus_tag="ACHKV2_005012"
     CDS             223..984
                     /gene="ydeO"
                     /locus_tag="ACHKV2_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO6942717.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
     gene            1388..3070
                     /gene="ydeN"
                     /locus_tag="ACHKV2_005013"
     CDS             1388..3070
                     /gene="ydeN"
                     /locus_tag="ACHKV2_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6942718.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            3122..4279
                     /gene="ydeM"
                     /locus_tag="ACHKV2_005014"
     CDS             3122..4279
                     /gene="ydeM"
                     /locus_tag="ACHKV2_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6942719.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4245..4367)
                     /locus_tag="ACHKV2_005015"
     CDS             complement(4245..4367)
                     /locus_tag="ACHKV2_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942720.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT         1364 a          910 c          877 g         1298 t
ORIGIN      
        1 aagaaatcat gtctgtaatt gtggttatct tattactgac ccttacgttg atagccattt
       61 tttcggcaat tgatcagctg ggtatttcag aaatgggtcg cattgcaaga gatcttacac
      121 atttcattgt caatagtttg caagactgaa aataaacagc aaattataaa tatgaaatgt
      181 taaaaaagta tcgataaaac tttattgttt taaggagata aaatgtcgct cgtttgttct
      241 gttatattta ttcatcatgc cttcaacgct aacattttag ataaagatta cgccttctct
      301 gacggcgaga tcctgatggt agataacgct gttcgtacgc attttgaacc ttatgagcgg
      361 cattttaaag aaatcggatt taatgaaaat accattaaaa aatatctaca atgcactaac
      421 atccagacag tgacgatgcc tgttcctgcg aagtttttac gtgcttcgaa tgtaccgact
      481 ggattgctta atgaaatgat tgcttatctc aactcggaag aacgcaatca tcataatttt
      541 tcagaacttt tgcttttttc ttgcctgtct atttttgcca catgcaaagg tttcattaca
      601 ctattaacta acggtgtgct atccgtttcg gggaaagtga gaaatattgt caacatgaag
      661 ctggcgcacc catggaagct gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg
      721 ttgaagaaaa aactaaagca agagcaaacg acattctcac agattctttt agatgccaga
      781 atgcagcacg caaaaaattt gatacgcgta gaaggttcag tcaataaaat tgccgaacaa
      841 tgtggttatg ccagtacatc ttattttatt tatgcgttcc gcaaacattt cggcaacagt
      901 ccaaagagag tttctaagga gtaccgttgt caaaggcaca cgggtatgaa tacgggcaac
      961 acgatgaatg ctttagctat ttgattattt gctaacgagt agtcaaccac gcaccctacg
     1021 taaaaattaa aaatggggca gtcattccct gccccgcgtt gtttttaggc gatatattta
     1081 ttgaaataaa taagtgacat tcatcacata tttatgcatt tgcataactt gttgcatgat
     1141 tatttatgat ctcaattcgg cattttgtta gtaaaataca ataatttatt aaatatcaat
     1201 agattaattg tttatcgtcg agaaattact tagtagaata aaaagtaatg tcaacgcttt
     1261 atggactgtt atttcccttt ttttagctaa atctgctatc tctttatgtg actaacttca
     1321 cttacatcca cttatttctc ttcgtaaaat tactttggaa ttaagtacaa taagaagagg
     1381 aacatttatg aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact
     1441 ggcatctggt atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac
     1501 caaaacaaac gttgctttct cagactttac gccgactgaa tacagtacca aaggaaagcc
     1561 aaatattatc gtactgacca tggatgatct tggttatgga caacttcctt ttgataaggg
     1621 atcttttgac ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat
     1681 agataaagcc attgaagctg cgcaaaaatc aacgccgacg ctcctttcat taatggatga
     1741 aggcgtacgt tttactaacg gctatgtggc gcacggtgtt tccggcccct cccgcgccgc
     1801 aataatgacc ggtcgagcgc ccgcccgctt tggtgtctat tccaatactg atgctcagga
     1861 tggtattccg ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac
     1921 tgcagcagta ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa
     1981 acaaacacgt gactatcatg acaacttcac cacattttct gcggaagaat ggcaaccgca
     2041 aaaccgtggc tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc
     2101 cccttcactg ttcaagaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt
     2161 aaccgatgag gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct
     2221 ttacctggct tataatgctc cgcacctgcc aaatgataat cctgcaccgg agcaatatca
     2281 gaagcaattt aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt
     2341 tgatcagggt gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac
     2401 aattattctc tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg
     2461 ggcgcaaaaa ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat
     2521 gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt
     2581 ctacccgaca gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg
     2641 cgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac
     2701 ctggataacc tcttattctc actggtttga tgaggaaaat attccattct gggataacta
     2761 ccacaaattt gtccgtcatc agtcagacga ttacccgcat aaccccaaca ctgaggactt
     2821 aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa
     2881 caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc
     2941 caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca
     3001 gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga
     3061 agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga gcgagatagc
     3121 gatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt
     3181 ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa
     3241 agagttcatc aaacaatata tcgcagcgtc tggcaatcag gtctatttta cctggcaagg
     3301 cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg
     3361 ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa
     3421 tgaatggtgt gcctttctca aagaacatga gtttctggtt ggtatctcga tcgatggacc
     3481 ccaggagcta catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt
     3541 gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat
     3601 taataacgtt aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag
     3661 taaacatatg caatttatcg aattgctcga aaccgggacg ccgaatattg atttcagtgg
     3721 tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa
     3781 gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcatccg
     3841 tcagtttgaa agctttgtca gccgtttttt ggggaatggg cataccagtt gtattttcca
     3901 ggagtcctgc aaggataatc tggttgttga aagtaatgga gatatttacg aatgcgatca
     3961 ttttgtctat ccacagtaca aacttggaaa cattaataaa tctgaactta aaacgatgaa
     4021 cagtgtacaa ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata
     4081 taaacctatc tgcaatggcg gttgccctaa gcatcgtatt actaaagtaa acgatgagac
     4141 tgtttcctat ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa
     4201 cgccatggtg gagttagcca agaacagagt accgctttac cacattatgg atgttgcaaa
     4261 acaaatggag aataattaat aacgctctct tttagctcca ttcttagcct ccttcttatg
     4321 accttaacaa ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct
     4381 tgcgaaaatc ttggcttacg tggtatgcgt cagggcatta gcaataccct tgattatcaa
     4441 tagcataac
//
LOCUS       DAZFTN010000226         4395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFTN010000226 DAZFTN010000000
VERSION     DAZFTN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..636
                     /locus_tag="ACHKV2_005016"
     CDS             <1..636
                     /locus_tag="ACHKV2_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6942721.1"
                     /translation="TVARLMAVMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERP
                     DQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWQVSSSMETTFVLDALEQALWARRP
                     SGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRK
                     SWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNNDLAA"
     gene            679..822
                     /locus_tag="ACHKV2_005017"
                     /pseudo
     CDS             679..822
                     /locus_tag="ACHKV2_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(824..1435)
                     /gene="emtA"
                     /locus_tag="ACHKV2_005018"
     CDS             complement(824..1435)
                     /gene="emtA"
                     /locus_tag="ACHKV2_005018"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO6942722.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1535..2449
                     /gene="ldcA"
                     /locus_tag="ACHKV2_005019"
     CDS             1535..2449
                     /gene="ldcA"
                     /locus_tag="ACHKV2_005019"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO6942723.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            2545..4281
                     /gene="cvrA"
                     /locus_tag="ACHKV2_005020"
     CDS             2545..4281
                     /gene="cvrA"
                     /locus_tag="ACHKV2_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO6942724.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
BASE COUNT          959 a         1094 c         1189 g         1153 t
ORIGIN      
        1 actgtggcac gtctcatggc agttatggga cttgccggtg ttctccgggg taaaaaggtc
       61 cgcactaccg tcagccggaa agccgttgcc gcatgcgacc gcgtaaaccg tcagttcgtg
      121 gcagaacgtc cagaccagtt gtgggtggct gattttacct gggtaagcac atggcagggc
      181 ttcgtttatg tggcgttcat cattgatgtg ttcgccggat acatcgtggg atggcaggtc
      241 tcatcatcca tggaaacaac attcgtgctg gatgcactgg agcaggcgtt gtgggcccgt
      301 cggccgtccg gcacaatcca tcacagtgat aaaggttctc agtatgtatc gctggcctac
      361 acgcagcggc ttaaggaagc cggattactg gcatcaacag ggagtactgg cgactcgtat
      421 gacaacgcga tggctgagag catcaatggt ctttacaaag cggaggtaat acaccgtaag
      481 agctggaaaa accggacaga agtggaactg gccacactaa cgtgggtgga ctggtataac
      541 aatcgacgat tgctggaaag gctgggccat attcctccgg cagaagcaga aaaagcttat
      601 tatgcttcca tcggaaataa tgatctggca gcctgagttc acagataaaa tactctccag
      661 gaaacccggg gcggttcaga tggcaagaat gaaaccatca ccactccccg tctgagcttc
      721 cgttttctta acgtcagccc gacggtggag cggcaattac agcggattat tttctctctc
      781 gagcgggaag cccgggagaa agcggacaaa gtacgcgact gatttacatc gcgtccagtg
      841 cctgctcaag tttgtagata tagcgcggag cctgcggcgc aggatgattt cgcgctacgt
      901 gatcgaggaa ctcgtcagca tccagatcgt tgattttgct gattgccttt ttccgatctg
      961 acgagaaagt ccgtagcagc gcacctgccc cgttagcata tgacaccacc agcgcatatt
     1021 gcagtacctt cggatcttca atgcctgcca gcgggccggt ttccagaata ttcaggtaag
     1081 ccgcccccat agaaatatta cgctccggat ttttcagctc gctggtcgtc ggctcaccac
     1141 tccagcccat acgccgataa acatcacgtc cggaggttga agcttttaac tgcatcaaac
     1201 caatggcatt cgatttactc accgcgttcg gattaccacc cgattcgata gcgataatcg
     1261 ccgtgatcaa ttgtggatcg acgccccagg ctgcaccggc tttctggctt attggcatcc
     1321 actgcatcgc acgttgcacc ggaactttcg cgttccacgg cgggttcgta tagtcatgct
     1381 ttgatgaaca acccgctaat aacacaatca aaaaggcaaa ccatctcaat ttcacttctt
     1441 ctatccttat agcctggaat cgccgttgag gtgacaacgg cacacctaag cggcatgata
     1501 tacatttcgt atctgttgtc agcaaggaat tgccatgtct ctgtttcact taattgcccc
     1561 atcgggttac tgcattaaac agcacgccgc gctgcgtggt atccagcgcc ttaccgacgc
     1621 ggggcatcaa gtcaataacg tcgaggttat tgcccgtcgt tgtgagcgtt ttgcgggtac
     1681 ggaaacggag cgtctggaag atctcaattc ccttgccaga ctgactaccc ccaacaccat
     1741 cgtgctggct gtacgcggcg gttacggtgc cagtcgttta ctggcagata ttgactggca
     1801 ggcactggtg gctcgccaac aatatgaccc gttgctcata tgcgggcata gcgattttac
     1861 cgccattcag tgcggtcttc tggcgcaagg caatgtcatc acctttagcg gccccatgct
     1921 ggtagcaaac tttggcgcgg atgaactgaa cacctttacc gagcaccatt tctggctggc
     1981 gttacgcaat gaaaccttta ccattgaatg gcaaggggaa ggcccgacat gtcgggccga
     2041 aggcacgttg tggggaggca atcttgcgat gctgatttca ctgatcggta caccgtggat
     2101 gccaaaaatt gagaacggta ttctggtgct ggaagatatt aacgagcacc ctttccgcgt
     2161 cgaacgtatg ctgttgcagc tctatcatgc gggaattttg ccacgtcaga aggcgattat
     2221 tctcggtagc tttagcggca gtacgcctaa tgattatgac gcaggttaca acctggagtc
     2281 agtgtacgca tttttgcgtt cccgcctgtc gattccactc attaccggcc tcgattttgg
     2341 tcatgaacaa cgcacggtta cgctgccgtt gggcgcacat gccattctta ctaacacccg
     2401 ggaaggcact cagttgacga ttagcggtca tcctgttctt aaaatgtaaa aaaaatcacg
     2461 cttcagggct aagtaaaacg ctgtctctgc cgctaatatg gtagggttta tataagagac
     2521 agcgtaatca ggagtaaccg accgttggat gccacaacaa taattagcct tttcatctta
     2581 ggatccatcc ttgttaccag cagtatatta cttagttcat tttcttctcg tcttggcatt
     2641 cctattctgg ttatttttct ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt
     2701 atcccatttg ataattaccc cttcgcctac atggtaagta acctggcgct ggcgattatt
     2761 ttgctcgatg gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt aggaccggcg
     2821 ctgtcgctgg cgacgctggg cgtacttatc acctctggtt taaccggcat gatggcggcg
     2881 tggctgttta atcttgattt gattgaaggc ttattaatcg gcgctatcgt cggctccacc
     2941 gatgctgcag cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg
     3001 acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg tctttctgac gattaccctg
     3061 attgcgatga tccagcagca tgaaagcagt gtcagttgga tgttcgtggt cgatattctg
     3121 cagcaatttg gcctcgggat tgtcatcggt cttggcggtg gttatttact gctgcaaatg
     3181 attaatcgca tcgccctgcc cgccggttta tatccattgc tggcattaag cggcggtatt
     3241 ttaatttttg ctttaaccac ggcgctggaa ggtagcggta ttctggctgt ttatctgtgc
     3301 ggttttctgc tgggtaatcg gccgattcgc aaccgctacg gcatcctgca aaatttcgac
     3361 ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc tggggctgtt ggttaaccca
     3421 agcgatctgc tgcctattgc cattccggcg ctcattttgt ccgcatggat gattttcttc
     3481 gcccgtcctc tttcggtatt tgccggattg ctaccattcc gtggcttcaa tctgcgtgag
     3541 cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc cgattattct ggccgtgttc
     3601 ccgatgatgg cggggctgga gaatgcacgt ctgttcttta atgtcgcctt ctttgtggtt
     3661 ctggtttcac tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg
     3721 gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc tggatattca tccggaaaat
     3781 ccgtgggagc agtttgttta tcaattgagt gccgataaat ggtgcgtggg cgcggcactg
     3841 cgcgatttgc atatgccaaa agagacgcgt attgcggcac tgtttcgtga taaccagttg
     3901 cttcatccta ccggcagcac ccgactgcgc gaaggcgatg tgttgtgtgt aattggtcgg
     3961 gaacgcgatc tcccggcgct cggtaaactg ttcagccagt cgccgccggt cgcgctggat
     4021 caacgcttct ttggtgactt cattctcgaa gccagcgcca aatatgcaga tgtggcgctg
     4081 atatatggcc tggaagacgg gcgagaatat cgtgataagc aacaaacgct gggtgaaatt
     4141 gtccagcagt tgttaggcgc ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg
     4201 atctggacgg tggccgagaa agaagataat gaagtgttga agattggcgt tcgagtggct
     4261 gaagaggaag ctgaatctta aatttaatga agcatctatc gccggttacg atgctttgct
     4321 taacgcagcc tgagcctgta tataaacttg tcatttcctg attttgatat gcacattcca
     4381 acatcagaag aaggt
//
LOCUS       DAZFTN010000227         4370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFTN010000227 DAZFTN010000000
VERSION     DAZFTN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..570
                     /locus_tag="ACHKV2_005021"
     CDS             <1..570
                     /locus_tag="ACHKV2_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6942725.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..4029
                     /locus_tag="ACHKV2_005022"
     CDS             631..4029
                     /locus_tag="ACHKV2_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6942726.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            4096..>4370
                     /locus_tag="ACHKV2_005023"
     CDS             4096..>4370
                     /locus_tag="ACHKV2_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6942727.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSV"
BASE COUNT         1024 a         1103 c         1343 g          900 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac caggctattt tcagataacc
     2821 gccacgccgc atcttgcggt ttatgatccg acggtacagt ttgagttctg gttctcggaa
     2881 acgcggattg cggatatcag gcaggttgaa accagcgcgc gttatcttgg tacggcgctg
     2941 tactggatag ccgccagtat caatatcaaa ccgggccatg attattactt ttatatccgc
     3001 agtgtgaaca ccgttggcaa atcggcattc gtggaggccg tcggtcgggc gagcgatgat
     3061 gcggaaggtt atctggattt tttcaaaggc aagataaccg aatctcatct tggtaaagag
     3121 ctactggaaa aagttgacct gacggaggat aacgccagca gactggatga gttttcgaaa
     3181 gagtggaagg atgctaacga taagtggaat gccatgtggg gcgtcaaaat tgagcagacc
     3241 aaagacggca aacattatgt cgcgggtatt ggcctcagca tggaggacac ggaggaaggc
     3301 aagctgagcc agtttctggt tgccgccaat cgtatcgcgt ttattgaccc ggcaaacggg
     3361 aatgaaacgc cgatgtttgt ggcgcagggc aaccagatat tcatgaacga cgtgttcctg
     3421 aagcgcctga cggcccccac cattaccagc ggtggaaatc caccggcatt ttccctgaca
     3481 ccggacggaa agctgactgc taaaaatgcg gatatcagtg gcagtgtgaa tgcgaactcc
     3541 gggacgctca acaacgtcac gattaatgag aactgtcaga ttaaggggaa actgtcagcc
     3601 aatcagattg aaggcgatat tgtcaaaacg gtcagcaagt ctttcccccg cacgagcact
     3661 tatgccagtg gcaccatcac ggtaagaatc agtgatgatc agaaatttga ccggcaggtc
     3721 atgataccgc cagtgttatt ccgcggtggt aagcatgaga atttcaacag taataaccaa
     3781 cagtcatact ggtattcaac ctgccggtta agagtgaccc gcaatggcca ggagattttt
     3841 aatcagtcca cgacggatgc tcagggcgta ttttcctcag ttatagatat gcctgccgga
     3901 caggggacgc tgacactgac attcaccgta tcttcatcag gagcgaataa ctggacacca
     3961 acaaccagta tcagcgatct gctggttgtg gtgatgaaaa aatccacagc aggtatcagt
     4021 atcagctgaa ttttataacc cagaacgggc gtcagaaatg acgccttttt tattgcagaa
     4081 aagcgagagg taattatgcg taaactttat gccgccattt tgtccgcagc catttgtctg
     4141 gccgtatccg gtgcgcctgc atgggcatct gaacatcagt ccacgctgag cgcggggtat
     4201 cttcatgccc ggaccaacgt tcccggcagt gatgatctga acgggattaa cgtgaaatac
     4261 cgttatgagt ttacggatac gctggggctg gtgacgtcat tcagctatgc aggagacaag
     4321 aatcgccagc ttacccgtta cagcgatacc cgctggcatg aagattccgt
//
LOCUS       DAZFTN010000228         4335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFTN010000228 DAZFTN010000000
VERSION     DAZFTN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..2812
                     /gene="eae"
                     /locus_tag="ACHKV2_005024"
     CDS             8..2812
                     /gene="eae"
                     /locus_tag="ACHKV2_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFO6942728.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3076..4296)
                     /gene="escD"
                     /locus_tag="ACHKV2_005025"
     CDS             complement(3076..4296)
                     /gene="escD"
                     /locus_tag="ACHKV2_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO6942729.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT         1271 a          847 c          940 g         1277 t
ORIGIN      
        1 ccataacatg attactcatg gttgttatac ccggacccgg cacaagcata agctaaaaaa
       61 aacattgatt atgcttagtg ctggtttagg attgtttttt tatgttaatc agaattcatt
      121 tgcaaatggt gaaaattatt ttaaattggg ttcggattca aaactgttaa ctcatgatag
      181 ctatcagaat cgcctttttt atacgttgaa aactggtgaa actgttgccg atctttctaa
      241 atcgcaagat attaatttat cgacgatttg gtcgttgaat aagcatttat acagttctga
      301 aagcgaaatg atgaaggccg cgcctggtca gcagatcatt ttgccactca aaaaacttcc
      361 ctttgaatac agtgcactac cacttttagg ttcggcacct cttgttgctg caggtggtgt
      421 tgctggtcac acgaataaac tgactaaaat gtccccggac gtgaccaaaa gcaacatgac
      481 cgatgacaag gcattaaatt atgcggcaca acaggcggcg agtctcggta gccagcttca
      541 gtcgcgatct ctgaacggcg attacgcgaa agataccgct cttggtatcg ctggtaacca
      601 ggcttcgtca cagttgcagg cctggttaca acattatgga acggcagagg ttaatctgca
      661 gagtggtaat aactttgacg gtagttcact ggacttctta ttaccgttct atgattccga
      721 aaaaatgctg gcatttggtc aggtcggagc gcgttacatt gactcccgct ttacggcaaa
      781 tttaggtgcg ggtcagcgtt ttttccttcc tgcaaacatg ttgggctata acgtcttcat
      841 tgatcaggat ttttctggtg ataatacccg tttaggtatt ggtggcgaat actggcgaga
      901 ctatttcaaa agtagcgtta acggctattt ccgcatgagc ggctggcatg agtcatacaa
      961 taagaaagac tatgatgagc gcccagcaaa tggcttcgat atccgtttta atggctatct
     1021 accgtcatat ccggcattag gcgccaagct gatatatgag cagtattatg gtgataatgt
     1081 tgctttgttt aattctgata agctgcagtc gaatcctggt gcggcgaccg ttggtgtaaa
     1141 ctatactccg attcctctgg tgacgatggg gatcgattac cgtcatggta cgggtaatga
     1201 aaatgatctc ctttactcaa tgcagttccg ttatcagttt gataaatcgt ggtctcagca
     1261 aattgaacca cagtatgtta acgagttaag aacattatca ggcagccgtt acgatctggt
     1321 tcagcgtaat aacaatatta ttctggagta caagaagcag gatattcttt ctctgaatat
     1381 tccgcatgat attaatggta ctgaacacag tacgcagaag attcagttga tcgttaagag
     1441 caaatacggt ctggatcgta tcgtctggga tgatagtgca ttacgcagtc agggcggtca
     1501 gattcagcat agcggaagcc aaagcgcaca agactaccag gctattttgc ctgcttatgt
     1561 gcaaggtggc agcaatattt ataaagtgac ggctcgcgcc tatgaccgta atggcaatag
     1621 ctctaacaat gtacagctta ctattaccgt tctgtcgaat ggtcaagttg tcgaccaggt
     1681 tggggtaacg gactttacgg cggataagac ttcggctaaa gcggataacg ccgataccat
     1741 tacttatacc gcgacggtga aaaagaatgg ggtagctcag gctaatgtcc ctgtttcatt
     1801 taatattgtt tcaggaactg caactcttgg ggcaaatagt gccaaaacgg atgctaacgg
     1861 taaggcaacc gtaacgttga agtcgagtac gccaggacag gtcgtcgtgt ctgctaaaac
     1921 cgcggagatg acttcagcac ttaatgccag tgcggttata ttttttgatc aaaccaaggc
     1981 cagcattact gagattaagg ctgataagac aactgcagta gcaaatggta aggatgctat
     2041 taaatatact gtaaaagtta tgaaaaacgg tcagccagtt aataatcaat ccgttacatt
     2101 ctcaacaaac tttgggatgt tcaacggtaa gtctcaaacg caagcaacca cgggaaatga
     2161 tggtcgtgcg acgataacac taacttccag ttccgccggt aaagcgactg ttagtgcgac
     2221 agtcagtgat ggggctgagg ttaaagcgac tgaggtcact ttttttgatg aactgaaaat
     2281 tgacaacaag gttgatatta ttggtaacaa tgtcagaggc gagttgccta atatttggct
     2341 gcaatatggt cagtttaaac tgaaagcaag cggtggtgat ggtacatatt catggtattc
     2401 agaaaatacc agtatcgcga ctgtcgatgc atcagggaaa gtcactttga atggtaaagg
     2461 cagtgtcgta attaaagcca catctggtga taagcaaaca gtaagttaca ctataaaagc
     2521 accgtcgtat atgataaaag tggataagca agcctattat gctgatgcta tgtccatttg
     2581 caaaaattta ttaccatcca cacagacggt attgtcagat atttatgact catggggggc
     2641 tgcaaataaa tatagccatt atagttctat gaactcaata actgcttgga ttaaacagac
     2701 atctagtgag cagcgttctg gagtatcaag cacttataac ctaataacac aaaaccctct
     2761 tcctggggtt aatgttaata ctccaaatgt ctatgcggtt tgtgtagaat aattccataa
     2821 ccaccccggc taaaatatgt attgttttag tcggggcata attatttctt cttaagaaat
     2881 aacctcttat aatcaaatct actactggtc tttttatctg cttaataggt ctctttcaaa
     2941 gagacacatt cacgttttct catgtaggtt gatccaacca cgctgatacc aaagctgaat
     3001 cacatcaagc aacaactatg ctcacaacat ccacacaata aaaaaccctc cgaagagggg
     3061 gaagaggata agaaattaat acgacagtgg aatatgtata tattcgtctg ggcgggagat
     3121 gttgatacca tctttaggat caatcgcgtc tatgacatag cctcctttca gaatgctacc
     3181 aatccccagg ctggtgccat tatcaagagc aattgttgga ttcttcatcg taccgctgat
     3241 tctgagtatt tttccgggga aaatatctgc tgaaatgtac ggctgaatgt tctgaaagag
     3301 tatcttggcg ttactatttt tgttcattgc atttataatg gcaaggatct tactctcatt
     3361 ctgttgagaa acttcccccg caacaatgat gacatcattc tttttgaaaa tattgacata
     3421 gtttattagt ttattctttt taaactctga tgccagttca ttgattttat tgttactgtt
     3481 gttgtgtatt tcccatcctg caactcctgg cgtattcagt aacagatttt caacctcgct
     3541 ccatttgggt gaagggggaa tgtatcccga taacagaaag aatccaggct tattaccttt
     3601 tgtaataatg atatctttgt aaccgtattc cgttaagaca tcattaattg cggatatgat
     3661 ttggttattg caaatgatat tattgtaata aaaaatattt ttttctttta ggtaattaaa
     3721 aaatggagtt aaatccgtgg ttgattcgca acgcccataa agcgcgatat ttttcccatg
     3781 ccataccaga gtgattgcat gaagtttgtt ttcttttagc tgctgatata catcagctct
     3841 ggtaaacgtt gggggttcag cttttgggga aagcaaaacg taactgccga tggcaccaga
     3901 caacgcaaaa gtagcaccaa tcacgctcgc caggataagg cgttttttgc gtcgactttt
     3961 ggcagagaac ctttctggca catcagacaa tggcaaggaa tggtcaatat caccaatgat
     4021 gaagcaacat cccgctatat caataacctt tccgacaggt aatggtttat ctgcttcata
     4081 gggcgtgggt tgcccatcaa tccaaaattc aaccggagat aaaaggaaaa caccttcctc
     4141 acgtatatca agtaaaaact gattgaggcc ttgttcaagg ggaaagtaaa caatatcgtt
     4201 atcttccgta cctagcgtta ggtttcccat cggaagttgt aattcccgat tacgcatcgc
     4261 accattgaga agttttattt tatatgagga taacattttt aaattttatt catcctggtg
     4321 gttgaacaat gagaa
//
LOCUS       DAZFTN010000229         4285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFTN010000229 DAZFTN010000000
VERSION     DAZFTN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..606
                     /locus_tag="ACHKV2_005026"
     CDS             <1..606
                     /locus_tag="ACHKV2_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFO6942730.1"
                     /translation="FSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDS
                     VINQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRIT
                     IEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEK
                     LNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(675..1631)
                     /gene="yjiA"
                     /locus_tag="ACHKV2_005027"
     CDS             complement(675..1631)
                     /gene="yjiA"
                     /locus_tag="ACHKV2_005027"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6942731.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1642..1845)
                     /locus_tag="ACHKV2_005028"
     CDS             complement(1642..1845)
                     /locus_tag="ACHKV2_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6942732.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(1895..4045)
                     /gene="btsT"
                     /locus_tag="ACHKV2_005029"
     CDS             complement(1895..4045)
                     /gene="btsT"
                     /locus_tag="ACHKV2_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO6942733.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
BASE COUNT         1110 a         1081 c         1117 g          977 t
ORIGIN      
        1 ttttctcaat ttattaccgc ctttgagaag gtatatatgc atgggtggtt aaaaaaacaa
       61 attaaaagcc aaagagaaat ggtttgttat tcagctctgg tagcaataaa taatgatatg
      121 ccttttgatt ctgtgataaa tcagataaac caacatgcag ataattcagg ttttatagct
      181 gctctggatg aagatttata tgagcctcgt ccgaatcaag tgaacttaat aaaagcaatt
      241 ttgcttcgct tagatatgga gcaacaagat gagagcgtga ttaaaaccta tacggggaga
      301 atcacaattg agcatattct tcctcaagca ttagttaatg aatactggat taatagattc
      361 caacctcaag agcatgtcta ctggctgcat aaaataggta atttaacact cataagtggt
      421 tccaaaaatt ctgaagcaca acactatgat tttatcaaaa agaaatctat ttatgagaaa
      481 cttaatagta aaagctcttt tgatttaaca aaagatgttt gtaattcttc agaatgggga
      541 ttggctgaac tgaaaatgag acatgaaaaa atgaaaacgc aattgaagaa gctttggttg
      601 gtctgacata tctaaagagt tccactcgta ataatgttgc ggtggaactc tcaaaaagtt
      661 gatctttccc gaacttactt cctcaacccc gcaaacgctg cccgaatctc ttcctccggc
      721 aactgaatcc cgataaaaac cattgtgcta tgcggttttt catcgcccca cgggcggtcc
      781 cagtcggcgc tgtagaggcg ctggacgccc tggaacagca ggcggttagg ttcgccgtca
      841 atccacagca tccctttgta acgcagcagt ttatccgccg attccagcag caggttttcc
      901 atcacgcggg aaacttcgct gatatctacc gggtaatcca gttctaccac aatcgacgaa
      961 atatcgtttt gtttatccgc gataaagtgg aaacgcggtt tggtgctgac gacgttttct
     1021 tccagcataa aaccgttggt gttgaacagc aggcccaggt cgatgtcgcc gtgggtaacg
     1081 gtgtagaccg gtgcacgggc gttgatgcgc gccaggcgtt cgcgcagttt ttctgcttcg
     1141 cctgcgacgt cggttttggt cagcagaata cggtcggcgt agccaacctg ggactgggcg
     1201 atggtgaact ggttcatctg ctcatcggca tgtaccgcat ccaccagcgc aatcacgccg
     1261 tccagcaggt aacgctggca taaaatctca tgggagaaaa aggtctgaat aatcgggccg
     1321 ggatcggcca tgccggtgca ttcaatgacc agacgatcga actgaatatt gcccttatcg
     1381 agattatcca gcaggtcgag tagtgcgtct tccagctcgt tggagcgcga acagcagatg
     1441 cagccgttgg tcagcgtttt gatctgcgtg gcgcggtcgc caatcagttg atcatcaacg
     1501 gagacttcgc caaattcgtt ttcaatcacg gcaatcttgt agccgtgttg ttcgttaaga
     1561 atatggcgca gcagggtggt tttgcccgcg cctaaaaaac cggtgagtag ggtaactgca
     1621 atcgggttca tgctctctcc attaacaaca gcgcataccg cctttaccat cgccgccgta
     1681 gcgcgcgttc tggcgttcgc ggaagaattc ttcatagctc atgtacggct tgtcgggatg
     1741 gttggtcttc atatgctcga cgtagttgtc gtagtctgga atgccaatca acatcttcgc
     1801 cgcctggccg agatattttt ttgcctgtcc taagttacca aacatagttc actctgataa
     1861 gaacaaagcc ccgccgaagc ggggctaaac acggttagtg gtgcgaagag atcttcacgc
     1921 cgccttccgg gattggaacg tacggtgttt ctttgtcagt acgtttgtcg ctgttacgta
     1981 ccgcaagcca ggttttgaaa ccgtagaaga tgatgctgta caccacaatc aggaacagaa
     2041 tactcaggcc tgcgttggtg tagttgttca caacgatgtg gttcatgttg gcaatctgct
     2101 gcgccgtaag gtcagtaccg ttagcaatct tctctttgta ctggcttgcc atgtagaaga
     2161 agccttccat ctgcgggttg gtgctgaaca gtttcagacc cagcgcccag gtggtgcaga
     2221 taagcagcca tacagccgga acaacagtta cccagatgta ttgggtgcgc ttcatcttaa
     2281 tcagcacaac ggtgcccagt accagcgcta cggctgccag catctggttg gagataccga
     2341 acagcggcca caggctctta acgccgccca gcggatcgac cacgccctga tacagcaggt
     2401 agccccacag acccacacag cccgcagtac cgatgatacc agcaaccaga gagtcggttt
     2461 ttttcaggaa cgggatgaag ttacccagca ggtcttgcag cataaagcgg ccagaacggg
     2521 tacccgcatc cagcgcggtc aggatgaaca gggcttcgaa cagaataccg aagtgatacc
     2581 agaagcccat gtcagccatc ggcagcactt tgtggaacac atgagcgata cctaccgcca
     2641 gcgtcggcgc accacctgca cggttcagga cagaaggctc accaatgtct ttcgcggttt
     2701 gcaggatctg ctctggcgaa atcacgaagc cccaagagct gacggtcgct gccgcgtgtg
     2761 cggtaacgtc tttcagctgc gccatgatga tcggcgcgtt ctcgccaccc atttcatgca
     2821 ggttaggcat ggtgatgcca aggcccgcgg gcggggtgtt catcgcgaag taaagacccg
     2881 gttcgatgat ggatgcagca accagcgcca taatcgccac gaaggactcc atcagcattg
     2941 cgccgtagcc gatgaaacgc gcgtcggttt cgttagccag cagtttcggc gtcgtaccgg
     3001 aagagatcag cgcgtggaag ccagataccg caccacaggc gatggtgatg aacaggaacg
     3061 ggaacagagc gcctttccac agcgggccag taccgtcaat gtactgggtc atcgcaggca
     3121 ttttcagttc cgggttcagc accacgatac ccagcgccag gccgacgata acgccgatct
     3181 tcaggaaggt tgccagatag tcgcgtggtg cgaggatcag ccacaccggc agcagcgcgg
     3241 aaacaaacgc atagccaatc agcgcgaagg taatggtggt gtctttaaag gtcagtgccg
     3301 gaccccagta cggatcgtga gcaatcacgc caccgaagta gatagaggca accagcagca
     3361 cgataccaat aacagagact tcacccacac gccccggacg gataaagcgc atgtagatac
     3421 ccataaacag cgcaattggt acggttgagc aaacggtgaa gacaccccac ggactttctg
     3481 ccagggcttt aaccacaatc agcgccagga cggcgaggat gatgatcatg attaagaaac
     3541 agccaaacag cgcgatagtc cccggtactg gccccatctc ttctttgatc atctcaccga
     3601 gagatgcgcc gttacggcga gaggagataa acagcaccat aaagtcctga accgcaccgg
     3661 ccagcactac ccccgccagc agccacagcg tgccaggcag gtagcccatc tgcgcggcga
     3721 gaaccggtcc caccagcgga ccagcaccgg cgatagcggc gaagtggtga ccaaacaaca
     3781 cgtaacggtt ggtcggaacg tagttcagac cgtcgttgtt aataaccgca ggcgtcgcgc
     3841 gcgtggggtc gagtttcatc accttctggg cgatgtacag actgtagtag cgatacgcca
     3901 ccagatacac cgacacagag gcgaccacga tccacagggc gctgacgtgc tccccccgac
     3961 gtaatgcaac taccgcgagg cagaatgcac cgatgattcc gagaatcacc cagggtatgt
     4021 gcttgaatag ttttttcgta tccatagtaa aacctggcat gtattgatta atagttggcc
     4081 gaagccgttc taggtttgcg ttgcgtttga ggaggtaaat tgaccgctat cctgcaatga
     4141 tgctgccaga gttacgcgcg cgtaaagtaa ggtaaataac tgagtggtta ttttagggac
     4201 gtaagcggtc agttttgcgg ttgagcggtt ttgagcaagg tttatgaagg ttatttatgt
     4261 gatgatgatc acaaccgaag cgatt
//
LOCUS       DAZFTN010000230         4236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFTN010000230 DAZFTN010000000
VERSION     DAZFTN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..583
                     /locus_tag="ACHKV2_005030"
     CDS             <1..583
                     /locus_tag="ACHKV2_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942734.1"
                     /translation="NKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSDK
                     KIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDN
                     GTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGIV
                     NVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            663..1358
                     /locus_tag="ACHKV2_005031"
     CDS             663..1358
                     /locus_tag="ACHKV2_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6942735.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1387..3903
                     /locus_tag="ACHKV2_005032"
     CDS             1387..3903
                     /locus_tag="ACHKV2_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6942736.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3914..>4236
                     /locus_tag="ACHKV2_005033"
     CDS             3914..>4236
                     /locus_tag="ACHKV2_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942737.1"
                     /translation="MKRAPLITGLLLISTSCAYASSEGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKT"
BASE COUNT         1214 a          999 c          967 g         1056 t
ORIGIN      
        1 gaataaagtt acaaaaacag ctattgcggg cttacttgcg cttttcgcag gtaatgccgc
       61 tgcaaccgat ggtgaaatag tttttgatgg cgagatttta aaatccgctt gtgaaatcaa
      121 tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac agtttcgttc
      181 tgtaggcgat cgtagcccaa aaattccatt cactattcct ttagttaact gcccagtgac
      241 tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtttatggc tggaaacgcg
      301 ggataatggt accgtcccta acttccctaa cttggcaaaa gtgggctctt ttgctggcac
      361 agcagcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaacc ttatgccact
      421 aaatgctatg ggcaatgata acacagttta tcagatccct gcggactccg ctggaattgt
      481 caatgttgac ctcatcgctt actacgtatc aaccgttgaa gcatccgaaa tcaccccagg
      541 ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta cggcgttatc
      601 tgacctttca gataacgccc ttttccttcc tatttctcgt tgtatcaggt tgaaaaataa
      661 ctatgtcaaa acgaacattc gcgatgatat ttaccttgtt gtgtagcttc tgtatcggcc
      721 aggcgcttgc agggggtatc gttttacagc gaacgcgagt gatctatgat gccagccgca
      781 aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac accttattta ctgcaatcat
      841 gggtagataa tatagatggt acaagccgtg ccccatttat tataacccca ccgctaattc
      901 gtcttgaagc tggcgatgat tcatcactgc gaattattaa aacagctgat aacctgcctg
      961 aaaataaaga gtcgctgttc tacattaatg ttcgtgccat tccagcaaag aaaaaatcag
     1021 atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa atgttttatc
     1081 gccccgcaca cctgaaggga cgggtaagcg atgcgtggaa atcactggaa tttaaacgta
     1141 gtgatcattc actcaatata tataacccaa ctgaatatta cgtcgtattt gccggactag
     1201 cagtcgataa agccgatctc acaagcaaaa ttgaatatat cgcgcccgga gaacataaac
     1261 agttaccact tccagcatct ggcggaaaga acgtgaaatg ggctgcgatc aatgattatg
     1321 gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa cacaggtcat
     1381 cagggaatgc cacaacgaca ccaccaggga cataaacgca caccgaaaca gttggcgctc
     1441 attatcaaac gctgtttgcc gatggtgctc actggcagcg gcatgctttg cactaccgct
     1501 aacgccgaag agtattattt cgaccccatt atgctggaaa ccacaaaaag tggtatgcaa
     1561 acaaccgatc tgtcacgttt ttcaaaaaaa tacgcacaac taccaggaac ttatcaggtt
     1621 gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa ttacatttac cgccaatgca
     1681 gagcaacttc tgcagccaca gtttacggta gaacaactac gtgagctggg tattaaggtg
     1741 gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg tgatcaactc gcttgaacaa
     1801 atcattcccg gtacagctgc tgaatttgat ttcaatcatc agcgacttaa tttgagcatt
     1861 ccccaaattg cactgtaccg tgatgcaaga ggttacgtct ccccttctcg ttgggacgat
     1921 ggtataccaa cgctgtttac caactactcg tttacaggtt ctgataaccg ttaccgccag
     1981 ggcaatcgta gccaacgaca gtacctaaat atgcaaaatg gtgccaattt tggcccctgg
     2041 cgattacgta actattctac gtggacacgc aacgatcagg cgtcaagctg gaacactatc
     2101 agtagttatt tacaacgtga tatcaaggcg ttgaagtctc agttgcttct gggagaaagc
     2161 gccaccagcg gcagtatttt ttccagctac aactttactg gcgtgcaact cgcttccgac
     2221 gataatatgt tgccaaacag ccagcgcgga tttgccccaa cggtacgcgg tatcgcaaac
     2281 agtagtgcaa tcgtgactat caggcaaaat ggttatgtga tctatcaaag caacgtgcca
     2341 gcgggtgcct ttgaaattaa cgatctctac ccctcttcca acagcggcga tttagaagtc
     2401 acgattgaag aaagtgacgg tacgcaacgt cgctttatcc agccttattc ttcattaccc
     2461 atgatgcagc gacctgggca tctaaaatat agcgcgaccg ctggacgcta tcgcgctgat
     2521 gcaaacagtg atagcaagga acccgaattt gctgaagcca cggcaatata tggtttgaat
     2581 aatactttta cgctgtatgg cggcctgctc ggttctgaag attattatgc gctggggatc
     2641 ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga tggatatcaa cagagctgac
     2701 acccaattcg ataaccagca ctcttttcat ggctatcaat ggcgtacgca gtacatcaaa
     2761 gatatcccgg aaaccaacac caatatcgct gtcagctact atcgctatac caacgatggc
     2821 tattttagtt ttgatgaagc caatacccgc aattgggact ataacagtcg ccaaaaaagt
     2881 gaaattcaat tcaacatcag ccagacaata tttgatgggg taagtctgta tgcctccggt
     2941 tcacagcaag actattgggg caataacgag aaaaacagga atatctctgt tggggtttcc
     3001 ggccagcaat ggggaattgg ttacagcctg aattatcaat acagccgcta cactgatcaa
     3061 aataatgacc gcgcactctc tttgaatctc agtattccgt tagaacgctg gttaccgcgt
     3121 agccgggttt cctatcagat gaccagccag aaagatcgcc caacccaaca tgaaatgcgt
     3181 cttgatggct cactgctgga tgatggtcgc ctgagctata gtctggaaca aagtctggat
     3241 gacgataaca accataacag tagcgtgaac gccagttacc gttcacctta tggaaccttc
     3301 agtgccggat acagttacgg taatgacagt agccaataca attacggcgt taccggcggc
     3361 gtggttatcc atcctcatgg tgtgacgctc tcgcaatatc tgggcaacgc ttttgcgctt
     3421 attgatgcta acggggcatc tggcgtgagg atacaaaact atccggggat tgctactgat
     3481 ccctttggct atgcagtggt tccttatctc acaacttatc aggaaaaccg tctctcggta
     3541 gatactacgc agctgcccga taacgtcgat cttgaacaaa caacacagtt tgtggtgccc
     3601 aacagaggtg caatggtagc ggcgcgtttc aacgccaata tcggttatcg cgtacttgtt
     3661 acagtcagcg atcgcaacgg taaaccgttg ccctttggcg ctcttgccag caacgatgat
     3721 acggggcaac aaagtatcgt cgatgagggc ggcatactat atctctctgg gatatcgagt
     3781 aaatcacaaa gctggactgt acgctgggga aatcaggcag atcaacaatg tcagtttgct
     3841 tttagtacac cggattcaga accaacaacc tctgtattac aaggcacagc gcagtgccat
     3901 taaggataaa aaaatgaaaa gagcgcctct tataacagga cttttgttga tatccacatc
     3961 ctgcgcttat gcctcctcag aagggtgtgg agctgacagc actagcggtg cgacaaatta
     4021 cagcagtgtg gttgatgatg ttacggtgaa ccagacagat aacgtgacag gacgggagtt
     4081 tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct ctgcgggtaa
     4141 ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac atcagacagg
     4201 atattacaaa ctcaatgaca gcctggatat taaaac
//
LOCUS       DAZFTN010000231         4217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFTN010000231 DAZFTN010000000
VERSION     DAZFTN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHKV2_005034"
     CDS             complement(<1..209)
                     /locus_tag="ACHKV2_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6942738.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKV2_005035"
     tRNA            complement(324..399)
                     /locus_tag="ACHKV2_005035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKV2_005036"
     tRNA            complement(406..480)
                     /locus_tag="ACHKV2_005036"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKV2_005037"
     tRNA            complement(597..681)
                     /locus_tag="ACHKV2_005037"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKV2_005038"
     tRNA            complement(690..765)
                     /locus_tag="ACHKV2_005038"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKV2_005039"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKV2_005039"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO6942739.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKV2_005040"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKV2_005040"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO6942740.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKV2_005041"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKV2_005041"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO6942741.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1083 a         1047 c          944 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaagaccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZFTN010000232         4160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFTN010000232 DAZFTN010000000
VERSION     DAZFTN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..2392)
                     /locus_tag="ACHKV2_005042"
     CDS             complement(59..2392)
                     /locus_tag="ACHKV2_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO6942742.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(2406..2729)
                     /locus_tag="ACHKV2_005043"
     CDS             complement(2406..2729)
                     /locus_tag="ACHKV2_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO6942743.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            complement(2729..2950)
                     /locus_tag="ACHKV2_005044"
     CDS             complement(2729..2950)
                     /locus_tag="ACHKV2_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942744.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            complement(2947..3504)
                     /locus_tag="ACHKV2_005045"
     CDS             complement(2947..3504)
                     /locus_tag="ACHKV2_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6942745.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            complement(3501..3761)
                     /locus_tag="ACHKV2_005046"
     CDS             complement(3501..3761)
                     /locus_tag="ACHKV2_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942746.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
BASE COUNT         1033 a         1122 c         1021 g          984 t
ORIGIN      
        1 tattttgaga taatttcctg tagcggattt aagaccggca cagccggtct taggtcagtt
       61 atttcgctgc aaactcatcg cattttggca accagtcagc attgctttcc tctcttaggg
      121 ccagattagt ctgcattcct tgattggtcc gtcgtttctc atactgcagc ccatattcct
      181 tgagcattaa cggtagcccc ttgccaaaca tggtcagact gagcgtattt ttatagccgt
      241 tagcctccat gtagaccaga taggcatggt agaggtaagt tcgaggctga cgtggaacaa
      301 tattagcgtt ccccataaac atgcccgtgg tgtcaggtag gacctcaagg tagccgcaaa
      361 aatcaaaagc tgaatcagca tcacgtttga tggtgagcgc ttcatcggag ttctgctgtg
      421 actgaagcaa tgtcctggca ctcattgggt cgctgaaacg ctgcataagc tggcgaacaa
      481 tcaccgctag ctcactggca attttctctt tgagctgggt atcgcgttcc tccggggcta
      541 tctggtcggg gaaatgcagg atcacccttc ggcgtgaaac tccaccacta cgatcagtga
      601 agcgcatcgg attattgttc acagccagga tgaccgccgg gatgtgggtt gaataggcgt
      661 tctgatattt gggatcaacg gataccgcat cgccgccagt gatggctttt agtccggccc
      721 cgtcaccgct ccacttttcc tggtcgggaa gtcgaatcag tgaaaaacct attaacgcag
      781 ctcgttctct tggcgactca agcatttcaa tggttgccga ggtcgcgtta tcttcacccg
      841 ccagcatggt tgctatttca gcaagaatac tctttccact tccgccaggg ccagtgacct
      901 ccagaaacag ctgccagtca taacggttag ccagcaccat aaacaatgca gccagaataa
      961 tgtcccgttt ttcaggatta aaacctgcgg cacgatccag ccagcgccag aatgccgggg
     1021 catgggtttc cagtgactca ccgtcaacgg gctgcgtgta atccacctcg cacagcgtac
     1081 gtaaccagtg cttcttatcg tgcgggctga acaatcctgt ccgggtatcg agcacaccgt
     1141 tgcgaaatcc gataagttgc cgcgccggat tctgctgttg cggaacgatc agtttgaggg
     1201 tttccacgag agacgcaatt ttcgcggatg aaaaaggtgc gtcgaggcgc tgaaacagcg
     1261 cagcgacatc acgggcaaaa tcggcgtaat agataacttt ccaggccccc gcctcatagc
     1321 gtgaaaggag ctgcccgttc gggtctactg ccagtgaatt tttatagtga tccactaccc
     1381 tctgcgcttt ttcgctgacg ctcagcgcgg taaattcagc ttcgctcatt gtgtcgaagg
     1441 ggctggcaac agctggtttt attacttcat gaattgcatg ccgggtggct tcttcgccgt
     1501 tttgcgtgaa tgcatcgttc cagtcaccaa acacaggcgg gagcgccatt gtgcaattgc
     1561 aggccccggt aacagctgcg ccccttgttt gccccgcacc gttgagatcg cggtcagcag
     1621 caataattat ctggtgcgtt gggtactcac tgcaggcaat gctcgccagg gaaaggaaat
     1681 tgacggacga aaaggccacc atgacagcat cgccagtcag atagttgata gtaagtgcgg
     1741 tggcatatcc ttccgctatc cagagccgtt tggctgcttt ggcagtacct tcaaccaggt
     1801 aaaaggcatt cttaacctga ccgcctttaa ggaagcattt gcccccatta gcgttgatta
     1861 actgcagatt taccagctct ccatctgcat acaatggaac gacaagatct cccgcgcgaa
     1921 atgccacacc accgacttta tgcatagcgg ttaattcccg gcaaggcagt gcaggaaacc
     1981 ctttactcgt cagataggca ttgccagtgg tctggcggga cttatcaaac aaacgggcag
     2041 ccagcactgc tgcagctttt ctcccgtcct ctttttcagg agaaccagat tcaagcattc
     2101 cctgagatac tggcagcagg tttccgataa tgccgtttat tctgtctgcc gcttcactaa
     2161 tgcctacatc cagtacttta cttacaagcg ccaggccatc acctgccccg cactggttac
     2221 agaaccacgt tccccgccct tcctggtcat caaatcgaaa gcggtctttc ccggcacaga
     2281 ccgggcaggg ctggtgccga tttttcagta catttacgcc cagcgcaggt aaaattcttg
     2341 accagtgacc ccgagctgct ttctcagcct ggctaacttt cattcctgac attgtgcggc
     2401 cctccttagt gcagcgctgg tttgttgagg tgacgggaac aaagttcatc catgaccgtc
     2461 attccgagca gtgagagaac cggacaggct ttaagcggcc ccgactccat cagatccgaa
     2521 agcaacgcgc aggcaatttc cagccctctt tgctgcccat gctggcgcag atagaaccct
     2581 tccagctcgc gggctatggc atcttcaagt tcagcgagcg tcaggccgga ataacgttcc
     2641 tgatgattgc ttagagccag ccaggcacag gcaacggcac ggcgatacag agcggcacga
     2701 agtgccagaa gagattcaca gcatatcatc aggccacctc cgcattcatc aggtcgtcat
     2761 ggcagcgttg caccactcca tccagttgct ctgtcatcag ataaatgagg gaaaccagtt
     2821 gttcgcattg ggcacctgct ggcttttcat aacagtcctg cagggctgcc atgccagtta
     2881 catactcacc cacattgcgt aaatgtttca gccttacaac atcgtcgtag gaaatctcaa
     2941 actggttcat agcgtcacct ccccggcagg cagccgtgca gaaagggaga gtacataatc
     3001 tcggactagc aaacggcgcg cggaacgctc atcgcaggag acagtgcgaa gcatacagat
     3061 gcgggggtga gtgtcagcac ggcggattgc ggcaaacaca aagacatatt gcgggtgtga
     3121 cggggtgagg attgtagcca tgatggcagc ctccaataag tagcggttat tgctaccacc
     3181 ggaaacgcca atttcactgg tggcagcccg aacggggttg gcgtaaccgg ccttattgga
     3241 aaccggccag cccgaaggct gccccgcccg gactaccatt atctcgatag aaccatggtg
     3301 taagcataaa caccacagcc ctgaaaatgg ttgtgcctga gctacgacgt aaaaaaagac
     3361 gcaaggggcg tcaattgtcg ccaataagtt atacggaacg ccaattccgg ctgccgattt
     3421 tgcgacagcg gaaaaactat acctggaaat gacgacagta agcaagccag aataaaggta
     3481 tttaatgtat gcaggtatca tcatgcatca catccccgcc cacgggcgct gatgcggtcc
     3541 gccatccatg cgctgacctc agactgtgcc caggcgacat ttttaccgcc aagcgggatc
     3601 tgcttcggaa acgcatcgcg gctgatcagg tcgtaaacgg tcgaacggga caacccgcat
     3661 aagtgcatca cctcaggcag gcgtaaaaat cgctcatgat ggttcgatac aggaaataat
     3721 ggcgcagcag gagctggcgc agacacagaa atattacgca tttatctacc ctctttaatt
     3781 tccacaacag tccggatgaa tttatccggg ttcaggtagt tccttattat gtgtatataa
     3841 accacctgcg cacgcatttt ttatttagcg tcacatattg attttatgga atttaaagcc
     3901 cgattaaacc atataaaatg acataataaa taccatgcct gcggactata aatacaggag
     3961 cattaatagg aactaccatt cacccagggt ttactatcct atgggtcatt gcacctgata
     4021 gtgctaaccc caccgccaat acagcaaggc agtacaactt ctacacgagg ccttgcattg
     4081 caggccccaa tttccgggca tatttttgaa tcgcatccaa cttataaaca ttgggcctgc
     4141 ataaaaacct tgatattaaa
//
LOCUS       DAZFTN010000233         4139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFTN010000233 DAZFTN010000000
VERSION     DAZFTN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            227..835
                     /locus_tag="ACHKV2_005047"
     CDS             227..835
                     /locus_tag="ACHKV2_005047"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO6942747.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHKV2_005048"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHKV2_005048"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO6942748.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4139
                     /gene="selB"
                     /locus_tag="ACHKV2_005049"
     CDS             2321..>4139
                     /gene="selB"
                     /locus_tag="ACHKV2_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO6942749.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT          938 a         1062 c         1204 g          935 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtgacgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     1381 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc ggaaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt agacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggttctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg
     2821 cgagcacgcc agccaacata gtttccgcct cgcgattgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gccaatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact
     3241 ggtcttcgac cccccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc
     3301 tgcccgcaac acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaaacca gagtatctgc aatgcctggc ttccctcgcc agggcacaga atgatagcga
     3421 agcgttagcc gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcg
//
LOCUS       DAZFTN010000234         4107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFTN010000234 DAZFTN010000000
VERSION     DAZFTN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..517
                     /locus_tag="ACHKV2_005050"
     CDS             <1..517
                     /locus_tag="ACHKV2_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6942750.1"
                     /translation="ADNHTLSAGYAQSKVQDFKNIKGVNLQYRYEWDSPVSVVGSFSY
                     MKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFYGLVGISHTKAKGDYE
                     WRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNVGYEGTKADIYGKHSV
                     NGFTVGVGYRF"
     gene            653..1444
                     /locus_tag="ACHKV2_005051"
                     /pseudo
     CDS             653..1444
                     /locus_tag="ACHKV2_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1449..2819)
                     /locus_tag="ACHKV2_005052"
                     /pseudo
     CDS             complement(1449..2819)
                     /locus_tag="ACHKV2_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2839..3186)
                     /gene="tnpB"
                     /locus_tag="ACHKV2_005053"
     CDS             complement(2839..3186)
                     /gene="tnpB"
                     /locus_tag="ACHKV2_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6942751.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3183..3857)
                     /gene="tnpA"
                     /locus_tag="ACHKV2_005054"
     CDS             complement(3183..3857)
                     /gene="tnpA"
                     /locus_tag="ACHKV2_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO6942752.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          922 a          987 c         1112 g         1086 t
ORIGIN      
        1 ggctgataat catacgctat cggctggtta tgctcagagt aaggtacagg actttaagaa
       61 tattaaagga gtgaatctgc aatatcgata tgagtgggat tctccggtca gtgtcgtcgg
      121 gagtttttcg tatatgaaag gtgattgggc ggacagtcat cgcgatgaag ctgacgattt
      181 ttacagacat caggctgata ttaaatatta ttcatttctc gcaggcccag cttatcgtct
      241 gaatgactat atcagttttt atggtttggt ggggatatct catactaaag cgaagggaga
      301 ttatgagtgg agaaacagtg tgggtgctga tgagtctgat ggatatttaa gtgagtctgt
      361 atcaaaaaaa tccactgatt ttgcttatgc agctggtgtt attattaatc cgtggggcaa
      421 tatgtctgta aatgttgggt atgagggaac taaagcagat atatatggaa aacattcggt
      481 caatggattt actgttggag ttggttatcg tttttaatca ttagacggga ctggataatt
      541 gcacttgcgt agtgaagttt actaaatgtt tgtattttaa tgaagtattc gacatattcc
      601 cttcagacgg aatatgtcga cggttatggt tttactggag catgagtatg ttatggcgaa
      661 actgataaat atatcagcgt ccactgagga gagaatgacg ccaggggaga ggagggtggc
      721 ttcacgactg gagagctttc tgaatgatga ttgctttgtc tggtacgata tcccggtggg
      781 acggaaaaac cgacatccgg attttgtcat tattgacccg gataacgggc tggtatttct
      841 ggaggtcaag gactggactg tatccacatt acgtaaagca aatcaggagc aggttacact
      901 ggaaaccgac ggtcttctga aatcagaaat aaatccgctg gtacaggttc gtcgttatgc
      961 ctgtgatacg gttaatgcct tacctgctga cccgtgctta cggcagaatg acggtcagta
     1021 taaggggcgg ctgaacctgg cctgggcgta tggtgtggtg tttacccgca ttacccgtca
     1081 gcaactgaag gcactgacag ggaataatga gaatgcggtg gaaaagattt ttccttcagc
     1141 acagacaatc tgtcaggatg aaatgacgca gtctgtttta cctgaggtat tccggcaaaa
     1201 aattgccggg atgtttacca cggggttcag aacccgggta acaccccgga tgagggatat
     1261 tctgcgcgcc catctgtttc ctgaagtgac cgtgaaacag aactcacaaa tcaaaattat
     1321 ggatattcag caggagattc tggccagaaa tattggtgac gggcatcgtg tcattcatgg
     1381 tgtggccggt tccggaaaaa ccatgattct tttgttccgt tgcctttacc tggcagaaac
     1441 cacgtccggt agttacgccg ccccagtgca actacccgga gggcgttttc acacaggtta
     1501 ttgtcgatct ccacccagcc attgcggcag tattcgttca gagcgtccca ttgcttcagc
     1561 agataggtga acgctttcgc tgtatccgag tggcgcgaca gcgtgttcat ctgcccctgg
     1621 agccactcat acaacgactg cattagcggt accgttctgg cttttctgac cgccagtcgc
     1681 tcttctgccg gactgccgcg gatctcagcc tcgatagcgt acagttcccc gatacgacgg
     1741 agcgcttctc ccgttactgt cgttggatgg cggacatgta catcgtggat cttgcgtcgg
     1801 gcatgtgcca tacaagccgc ttcggttgcc tgaccgcttt cgtaaagagc attgtaaccc
     1861 gcatatgcat cggcctgcag gatacctctg tagtccgcca gatgttgctg tgggtggatg
     1921 cctttgcggt cgggagagta tgagaaccac accgctgccg gcatggtcga accggcgttg
     1981 cgatcgtcac gaacatagac ccacagccgt ccggtacggg ttttaccctg acccggctcc
     2041 agtacattta ccggcgtgtc atcggcatgc actttacccg gcatcagcac atagtgcttc
     2101 agttcatcat acagcgggcg aagctgctct cccatgatgt caacccagcg ccccatcgta
     2161 ttgcggtgca gctccacgcc ctggcgggca tagatttccg actgacggta cagcggcaga
     2221 tgctcggcga acttagccat gattatgcgg gccagcagag ccggactggc gtaactgcgc
     2281 tcgatgggtt ttggtggctg cggagcctga actatacagt cgcaccggct gcaggcccgt
     2341 tttgggcgaa ccgtttcgat taccctgaac gcggtgttga tgatatccag ttgttcagag
     2401 atgctttctc ccagcggttt cagtttaccg ctgcagacgg ggcattcggt ttctgccggg
     2461 gagataacct gcctgtcacg gggaagtgtt gccggaagtg ctttgcggac gggagagtct
     2521 gatgttttcg gcgctgtctc tccggccatt gaggtgagtt gcaactgcgc ctcaccaagc
     2581 ctgttctgga gctcggttat acgcgtttct gcccgtgcga tcttcttttc tatcttctcg
     2641 cggcttttct cgctgctgcg accgaacaac attctctgca gtttagcgac cagcgctctg
     2701 agtgagctga tctcgcgggc atagccggtt atttcaccag acagacggac gataacagcc
     2761 tgctgtgcga tcagcagggc cttcagttgc tcgatgttgt cggggagtgt gttgttcatt
     2821 cccctgtttt atcacgggtt atatccggat gccaggccgt tctgtccgtt tgggatgttg
     2881 ccatgcgatc ccctccagta gcatggataa ctgagctggc gtcaggtgca ctttcccttc
     2941 ccgggttacc ggccagacga agcggccccg ttccaggcgt ttggcgaaca ggcataaccc
     3001 gtcacgatcg gcccacagta ttttcaccat tttgccactg cggccccgga agacgaagat
     3061 atgcccggag aacggggcat ctttcagcgt gttctgcacc ttcgaagcca ggccgttgaa
     3121 gccacaacgc atatctgtga tgccagcgat gatccagatt ctggtaccgg ttggcagcgt
     3181 tatcatcggg tacctccttt catttcgcgg attagcgccc gtaacatttc cggagtgaga
     3241 gggtcaaaca gttttaccac acctgattta agatgcagct cgcaccgtgg gacgtttccg
     3301 ggatcactct cagggcactc atcaggcttg ttacgccaga agggatttgt aactggtctg
     3361 gtcggctctg gcgtatcagt cagagccacc gggacaggca tgcattcctg tatgtcatca
     3421 tcgctcagta agccgtcctc gtactggctt ttccatttta acagcaggtt atcattgata
     3481 ccgtgctctc tggcgatccg ggcaacaaca gcaccgggct gtaatgcctg ttttaccagc
     3541 ctgattttaa attcacgact gtaggaggtt cgtcgttctt ttcgccatgt gccttcgctg
     3601 atttgaggct ctgttaattc cttctttctg ttggcataaa ggatggcgtc aagctgagct
     3661 aatgaaactg aatcgggcaa tggccatgcg ataccggatg caataaatcg ctgaaaaagc
     3721 gtatgtattg tggaatgact gagacctaga cgctgagcga tggcccggat ggtcagttta
     3781 tcttcaaatc ttaaacgcag agcatcaggc aaataagaac ggaagcaggg aatatctttt
     3841 tttgtctggg aattcatcgt tcgtgtccat ctatatagat gggcgcgatt gttgccagac
     3901 aggacaattt tcacaagacg tcgcagatgg ggcgcttaca caacaatggc gcgagggaaa
     3961 gctgctatta ccttcttcag agagccccca gctacttcct gtgactctcg atgcagctgc
     4021 cgaacagcca gaatcgctcg cagaggaccc ggaaaccctc agtatcagct gttgtcaacg
     4081 acggatgact attgcaccgt tcgtgac
//
LOCUS       DAZFTN010000235         4071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFTN010000235 DAZFTN010000000
VERSION     DAZFTN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            93..809
                     /gene="nanC"
                     /locus_tag="ACHKV2_005055"
     CDS             93..809
                     /gene="nanC"
                     /locus_tag="ACHKV2_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO6942753.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            829..1935
                     /gene="nanM"
                     /locus_tag="ACHKV2_005056"
     CDS             829..1935
                     /gene="nanM"
                     /locus_tag="ACHKV2_005056"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6942754.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2000..2980
                     /locus_tag="ACHKV2_005057"
     CDS             2000..2980
                     /locus_tag="ACHKV2_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6942755.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            3103..3798
                     /locus_tag="ACHKV2_005058"
                     /pseudo
     CDS             3103..3798
                     /locus_tag="ACHKV2_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1145 a          925 c          957 g         1044 t
ORIGIN      
        1 taagcctggc aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat
       61 tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg
      121 tattattact gtgcttttcg tcaccattaa tttctcaggc tgcgacactg gacgtacgtg
      181 gtggatatcg tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc
      241 aaaatggatg gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa
      301 aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg
      361 atcaatggac ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg
      421 gaccctacgt aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc
      481 gttacgactg gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg
      541 ttcatcgttg ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc
      601 aaacgacgct atacagcaaa cagaacgatt atcgctatgc aaaccataag aaatgggcga
      661 cggaaaatgc atttgttcta caataccata tgacgcccga tattacgcca tacatagaat
      721 atgactacct ggaccgtcag ggtgtttaca acggcagaga taatttatct gaaaacagtt
      781 atcgcattgg tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaaca
      841 ataacggcgc ttgctatcct aatggcttca tttgccgcaa acgcgtctgt attaccggaa
      901 actcctgtac catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt
      961 ttaggtagcg caggtacggc atggtacaag ctggagacac aggccaaaga taaaaaatgg
     1021 acagcgttag ctgcattccc tggtggacca agagatcaag caacctcggc atttattgat
     1081 ggcaatctgt atgtgtttgg cggcattggc aaaaacagcg agggcttgac tcaggtattt
     1141 aatgacgtac acaaatacaa ccccaaaacc aatagctggg ttaaattgat atcgcacgcg
     1201 ccgatgggca tggcgggcca tgtgactttt gtacacaacg gcaaggctta tgttactggc
     1261 ggtgttaacc agaatatctt caatggctat tttgaagatc tcaacgaagc tggaaaagat
     1321 tcaaccgctg tagataaaat caatgcacac tattttgaca aaaaagcaga agattatttc
     1381 tttaataagt ttctgttgtc ttttgatccc tctacacagc aatggagtta cgctggcgaa
     1441 tctccctggt acgggacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt
     1501 attaatggcg aagccaaacc cggattgcga acggatgccg tatttgaact tgatttcacc
     1561 ggtaataatt taaaatggaa taggcttgct cccgtctcat caccagatgg cgtcgctggc
     1621 ggttttgcgg ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt
     1681 tcacgagaaa attaccaaaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat
     1741 agcactgata ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa
     1801 ggtcgggcct acggagtatc attgccctgg aataatagtc tattgattat tggcggtgaa
     1861 actgcaggcg gcaaagcggt gacggattca gttttgatct ctgtgaagga taataaagtc
     1921 acagtacaaa actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt
     1981 tcaattacgc gaaatttata tgaacgcaat aatatcgcct gattattact atgttcttac
     2041 tgttgctggt cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga
     2101 agatgcgcct catcccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg
     2161 cccgtcatgt cactttaacg acattattcc actgactcac tgcccacacg atgttcagga
     2221 tatgcagagt tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg
     2281 ccaggcactg catattgcac ggaaattact gcccttcatt cctgataatg cagggattct
     2341 catcgttccg tgttgccgtg gcggatcggc ttttaccgca ggcagcgagg ggacatattc
     2401 agaacggcac ggagccagcc atgatgcttg tcgttgggga acggatactc cgctatacca
     2461 ggatttagtc agcagaacgc gagccgcact ggtaaaaaat ccgcagaaca aattcctcgg
     2521 cgtatgctgg atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacatcctca
     2581 acactttaat catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct
     2641 taataatatt actgacgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa
     2701 tttccctcat gcgtatgaag ctatttatgg caattatcaa aataatattt tagctaatat
     2761 tattttcgtc gacttccagc aacaaggtgc aagagggctg acgaacgcgc ctgatgaaga
     2821 tccggacgat ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac
     2881 gacggcactg cgaagcagtc atttcagctc agcagcccgt cgggggatta tttctgacag
     2941 gtttgtagaa gcaattttgc agttttggcg cgaaaggtga gcgttcatta atttgattta
     3001 ttcgcatcca tcagcgcttt taactcctcc ggcgtgcggc actataacga cttccacaac
     3061 aaggagccat tttcttatac cactttcagt gaccatccgc ttatgccacc accctctacg
     3121 caactgtttg cagagcacct tcccaccgag tggatacagc acttcctgac gctttctgct
     3181 catgcgaccg ttcgtcgtct taacctgagc gtggatggcg aagcggggat gaacctgctg
     3241 gcgcccgcag cgctgtcacc caggcgcgtc agcgcgtggg ggccgccccg gtggaatggc
     3301 tcttccgcca gaccacacag acatggggcg cggaacgtta cctgaaggat gactggcacg
     3361 gcctgcaact ttttgccatt gatggcgcac agttcaggac acctgatgaa cccgagctgc
     3421 gtgaatatta tggctctgcc aacacatcca ctgagcggca gagcgcctac cccgtcatgc
     3481 gtctggtggc gttaatgaat ctgggaatca cattctactg aatgccatga ccgcgcctac
     3541 cggcggagcg atacggcgtg aggcaacaaa agcggcgtca gagatcagct ttaagttcgc
     3601 gttccagttt atcgtgacag aaatgatagt gctgggaaac acggcgtcac cggggactat
     3661 cccgaaacga ctggagcatc tgcgggggct ctggaagtgg tgttcataac aaaacgcccc
     3721 cgaccatcaa gggcgagggc ggttaagata tcaaaaaccc gttatcctgt gaaacacagt
     3781 gccgccccgc ttaagtgaac ggcattgtgt ttctgaggga ccttaatttt ccctgattct
     3841 ctgacgttcc ttaccattga tatttccttc attcctcacc agagaaataa aaaatcacta
     3901 tactcatcaa aatatgagat tacctttatt caggtgatca ccttactatt ttttacggaa
     3961 aataaattat gaataacaag aaatcactga caaaccacct gtaaaatgtc aatcatttta
     4021 gttcaattgc ctttgtcatg attatctttt tatcgtcctg acgatactat t
//
LOCUS       DAZFTN010000236         4065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFTN010000236 DAZFTN010000000
VERSION     DAZFTN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(436..1413)
                     /locus_tag="ACHKV2_005059"
                     /pseudo
     CDS             complement(436..1413)
                     /locus_tag="ACHKV2_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1791..2465)
                     /gene="nleE"
                     /locus_tag="ACHKV2_005060"
     CDS             complement(1791..2465)
                     /gene="nleE"
                     /locus_tag="ACHKV2_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO6942756.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKIE"
     gene            complement(2514..3503)
                     /gene="nleB"
                     /locus_tag="ACHKV2_005061"
     CDS             complement(2514..3503)
                     /gene="nleB"
                     /locus_tag="ACHKV2_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO6942757.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            complement(3744..3929)
                     /locus_tag="ACHKV2_005062"
     CDS             complement(3744..3929)
                     /locus_tag="ACHKV2_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO6942758.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
BASE COUNT         1140 a          852 c          750 g         1323 t
ORIGIN      
        1 tgataaaaaa aatatttatc tcgatgctac ccaacatacg actcatttat ttttctcggt
       61 ttctccttgt atatatatgt tgctttataa ttttactacg cacgtcaggc gtatctgtaa
      121 catagctccg cattatgcaa tgcgactttg catacaaata ggaataaatc taatagctgt
      181 agatttgctc ctgtatttcc tgaaccaagc gagccagtac atgatgcata tgtctggaaa
      241 ataattcaga gtaaggataa gtccagcttt gttgaataaa tcaggtttgg aataagcccc
      301 ctcagttggt agctactgtc aggtaatacg cacgtattat gacattcgtg ccctcgtcat
      361 tttgtttagt acacgcactg tagtggcaca gaacttctgc gacctgacca tcctaaatag
      421 ctgcgccgaa taccgctaca cttttaacag cccatattta ccaccgggga atgggctcac
      481 ggtttccata aaataatgaa catttttaag cagttaccac attgaacggc actgatgatt
      541 gcgcgttatc gtatcgtgaa gcgcctgcta taaccgctca acatgattca cccacggcga
      601 gtaaactggc tgataaatca ctctgaactt cggattttct ttcagccagc gctgtgtttc
      661 ccgactttta tggatgatgt agttgtccac tatgagcgtg ataggtttat cccgtcggta
      721 tgtcaatttc agatgtttca acagactgat aaataaggct gagcttttgc tgttgccacc
      781 cacatagctg actttgcccg ttccactatg taaagcacca gccagatagt atttttcatt
      841 ctgccccggt gttaccacat gtttttgctg tctgcgaagt tgccagtccg caccggtttt
      901 gggattaaga tagatataca cttcatcttc ataaaagacc ggatgctccg cactacattc
      961 gtccagtgct ttgtgaattg ctgctttatg cgggttacgg gtacgcaggg ttggcgcagc
     1021 cctgcgccac acaagcccga ctaatggcaa ccagcggcga acggttccgg catgtaactg
     1081 gcactcggtt atctcgttga tttttattgc cagcagttct gtactccagc gtgaacgctg
     1141 atagccaaaa tatccgggag agtgttttat cagttcacga aacaatgtgc agatatgctc
     1201 aaacggccag cggcgggaac gtcctgcggg taatgatttc agaccttcaa cactcgacag
     1261 cgtgaaccag ttaatccagc gcctgacgga ggaacgggcg cagcagagcg ttctggcaac
     1321 gtcactgacg cgagcgctcc ggtgcagcat cagcatggcg gtcagcctgc gggcataatt
     1381 tttatcgtga gttttataga ttgctttttg catcaggcgt cattcgtcgc ggggaattgg
     1441 tgctatgatc ggcatcgctc agtccggtta gggggggatt tttggcgatt gatcagatcg
     1501 cacaatccgg gctgagttcc ctttcattga tctactattc ggcgcagcta tttagttggt
     1561 gattttgaac ttctgctttg ctactaagca gcctgcgttt tcaggaaaat acgtgatgtg
     1621 atcctgcaac tcagcaacag ttcggttgat tcaacaaagc gttttttaac aataattctt
     1681 cattgtaaaa agcatcttca gttgtgatca aatattatta ttcttattgg tgttctcata
     1741 taatgaaata gaattaattt tagcccccct acacaagtgg ctgagcttct ctactcaatt
     1801 ttagaaagtt tattatttat gtatttcata taactgtcta tttccccagg catgtagcta
     1861 aagatttctc ccatttcacg tgcgataatc atttcagatt ttactgcctt taactctggt
     1921 aactcataat ttaattcctg catgatatct ctttttatat tatataattc tataaatcta
     1981 tttgcccgtt gttctcctag agggctgtca tttctgtaca caacaactat gtataaaaaa
     2041 tcattatttt ctttagggaa gatcttacaa caaatgtccg gaagctcaca taatgcatgt
     2101 aaatcaggat tgtataaact atctacagaa aactgcccca ttgggcgttt tccggatata
     2161 actgttagcc agcttccttc cggtgtactg gcttcatttc tggaaatagg ttcaaacgct
     2221 tctggacata cagaatacat agtcttcata taagaaaacc gtctggcttt ctcgttaaca
     2281 aattcttcct gttttagttc agctactcct ctggttatac cggatatata cttcttatca
     2341 tatatattgg gtgattcatt aaagtaatca tgtttaattt ccgggtaaat atttttcacc
     2401 acatgttcag catatttagt atttataggg gacacgccct gagtattagt aacaggatta
     2461 atcatctgaa tctataccta aagaacgata tttttcaacc acaaccggaa gacttaccat
     2521 gaactgcagg tatacatact ggtattcggt ataatatttt catgcttaaa ttcaataaaa
     2581 tcacaaaaag cattataatc gtgtaacgat gaatagttaa aatgccgctt gatacccttt
     2641 cctagaccat cataatatgg atgagcgtca actttactct tcataatatc gaggcctgca
     2701 agtaatgctg gatgattact acgattgaca actatcgcac cattttcaag actttttatc
     2761 tcatcattac aatctacatg cacagcgata ccatcaggag catacaggac tcctaattta
     2821 tcggtaataa tcatgtcggc atcaagatat atacatccgc tacagggcga tatgttatgg
     2881 catccagttt ttcccgtctc tgtaaacaaa cttccagcct tcaacatggc caaattcctg
     2941 aaaaaatcaa accaagtatg ttctcttttt tctgcatatg actcaatcaa agagtcctta
     3001 ccttctggga tattttttaa ctcttctctt aataagccta acagataatt atccctctca
     3061 ctggcagggt atgattcttg tttatggata ttttcaaggt aaatatcaga tagtttttta
     3121 tcatacatgc tgaaatccac atctgctcgg tagattattt ttatattttg atattcattt
     3181 tcaagactga gaaattcctt tttttgttca taagtaaaat ttccatctac cagcacaccg
     3241 attatacgct cattttctat ttttgcagca tttataatat tgctcaaata cggatgttct
     3301 ttggtattca atattggtac ttctcctttt tcgtatcgct ctggccttgc ttcaaaccac
     3361 tgaaaaagaa taggggtttt ttcatcaata ggttccagag gatattcttt tccagcaaaa
     3421 gaaactttct ggagaagtgt actatttgat aaagctgtct ttcctctgaa gctggattga
     3481 aggacattta atgaagataa cataaaattc ctcttgttct aatcatgtta atatcgcccc
     3541 gtcatttaga cggaacacta cgtcagctta attgtttttc taaaataaat tcatagcatt
     3601 gttgacatta acaaaaagtc tcattaacga tgacatcctt tcccctccct gttctattgt
     3661 atagaatcgt aaccctgaat acaggttaga ttttaacaat cagcagagta atgttgtcaa
     3721 taagaaaaag gtgatggcaa taattaatag aatatatgtt ttttgataaa agtaccgatg
     3781 acttttttat tacgtactac agtgcgtgag aaatagatga ttctgcgggc cagacgttta
     3841 atccgggttc tctgagtcag attgtttcgc ccaatgagct tttgcaggaa aatgagtgat
     3901 ctgatcctta aactcagcaa aagctcgatt tattcaacaa agcctgtctg cgtttctccg
     3961 gcccaaaaat atcaatcatc tgttctaaaa aacagcatta agattatcac ttatttaagt
     4021 aatattggtt gtctgtagac tcagggggct aaaatacatg tgcct
//
LOCUS       DAZFTN010000237         4037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFTN010000237 DAZFTN010000000
VERSION     DAZFTN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..82
                     /locus_tag="ACHKV2_005063"
     tRNA            6..82
                     /locus_tag="ACHKV2_005063"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Arg,seq:tcg)
     gene            96..172
                     /locus_tag="ACHKV2_005064"
     tRNA            96..172
                     /locus_tag="ACHKV2_005064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Arg,seq:tct)
     gene            263..1222
                     /locus_tag="ACHKV2_005065"
     CDS             263..1222
                     /locus_tag="ACHKV2_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO6942759.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1234..1503
                     /locus_tag="ACHKV2_005066"
     CDS             1234..1503
                     /locus_tag="ACHKV2_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO6942760.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            1989..3926
                     /locus_tag="ACHKV2_005067"
     CDS             1989..3926
                     /locus_tag="ACHKV2_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6942761.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAAN
                     QYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGT
                     FGAFDNEWHMLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRN
                     ATYPVLIDSIAVEVNNADAAA"
BASE COUNT         1018 a          920 c         1121 g          978 t
ORIGIN      
        1 acataccgcc attagctcat cgggacagag cgccagcctt cgaagctggc tgcgcggggt
       61 tcgagtcctc gatggcggtc cattatctgc attatgcgtt gttagctcag ccggacagag
      121 caattgcctt ctgagcaatc ggtcactggt tcgaatccag tacaacgcgc catatttatt
      181 taccaggctc gcttttgcgg gcctttttta tatctgcgcc gggtctggtg ctgattactt
      241 cagccaaaag gaacacctgt atatgaagtg tatattattt aaatgggtac tgtgcctgtt
      301 actgggtttt tcttcggtat cctattcccg ggagtttacg atagactttt cgacccaaca
      361 aagttatgtc tcttcgttaa atagtatacg gacagagata tcgacccctc ttgaacatat
      421 atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca gttattttgc
      481 tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc gtctgattat
      541 tgagcaaaat aatttatatg tggccgggtt cgttaatacg gcaacaaata ctttctaccg
      601 tttttcagat tttacacata tatcagtgcc cggtgtgaca acggtttcca tgacaacgga
      661 cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa tgcaaatcag
      721 tcgtcactca ctggtttcat catatctggc gttaatggag ttcagtggta atacaatgac
      781 cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag ccttacgctt
      841 caggcagata cagagagaat ttcgtcaggc actgtctgaa actgctcctg tgtatacgat
      901 gacgccggga gacgtggacc tcactctgaa ctgggggcga atcagcaatg tgcttccgga
      961 gtatcgggga gaggatggtg tcagagtggg gagaatatcc tttaataata tatcagcgat
     1021 actggggact gtggccgtta tactgaattg ccatcatcag ggggcgcgtt ctgttcgcgc
     1081 cgtgaatgaa gagagtcaac cagaatgtca gataactggc gacaggcccg ttataaaaat
     1141 aaacaataca ttatgggaaa gtaatacagc tgcagcgttt ctgaacagaa agtcacagtt
     1201 tttatataca acgggtaaat aaaggagtta agcatgaaga agatgtttat ggcggtttta
     1261 tttgcattag cttctgttaa tgcaatggcg gcggattgtg ctaaaggtaa aattgagttt
     1321 tccaagtata atgaggatga cacatttaca gtgaaggttg acgggaaaga atactggacc
     1381 agtcgctgga atctgcaacc gttactgcaa agtgctcagt tgacaggaat gactgtcaca
     1441 atcaaatcca gtacctgtga atcaggctcc ggatttgctg aagtgcagtt taataatgac
     1501 tgaggcataa cctgattcgt ggtatgtggg taacaagtgt aatctgtgtc acaattcagt
     1561 cagttgacag ttgcctgtca gactgagcat ttgttaaaaa aatttcgcat ggtgaatccc
     1621 cctgtgtgga ggggcgactg gtgaaaaatc cttgcttgtg attcattatc gacacgggtt
     1681 cggtggtacc aggccgaact caccgggagg cacccggcac catgcagtat acagagatta
     1741 ggcatatacc aaggcctctc atagcagggg cctttttaca tgtaaaaaag cccgagtggg
     1801 ttcgggcaat tgcatgagat actcgtttta ataatcgaaa gcattttaac caggattcat
     1861 aaggctgcgc aactgcgcgg cctttttcgt atttcgggct gtagtcttcc ttctgccatt
     1921 gtcctgtaac ttccggactt cagcccgctc ctcatctgac tcacacatta tcccgaccgg
     1981 gaggattcat ggcatttaaa cactatgacg tggtcagggc ggcgtcgccg tcagaccttg
     2041 cggaacgaat aactcaaaaa ctgaaggaag ggtggcagcc ttatggtagt gcgctgattt
     2101 cgacagctgg ttatggtgcg gagttcatcc agccagttgt gagtgagggg agcatctcat
     2161 caccagagga gccaggcaac cgtccgacga cctcagcgcc ttctgttgcg ccagaatatt
     2221 actatgtgat cgcgcttgct ggtcagtcca atggtatgtc atacggtgag ggactgccat
     2281 taccggatac attcgacagt cctgatccac gaattaaaca gttagcgcgt cgcagtacgg
     2341 tgacaccggg aggtgccgcc tgtaagtata acgacatcat tccggcggac cattgtctgc
     2401 atgatgtgca ggacatgagc cgccttaacc atccgaaagc ggacctgtca aaggggcagt
     2461 acggaaccgt ggggcaaggg ctgcatatcg ccaaaaaatt gctgccgttt ataccggcga
     2521 atgcgggcat tctgctggtt ccgtgctgtc gtggtggttc agcgttcacc accggagccg
     2581 atggcacata cagtgacgcg agtggtgcct cggagaattc aacccgctgg ggtgtggaca
     2641 agccgctgta taaggacctt atcggtcgaa caaaagcagc actgaagaag aatccgaaaa
     2701 atgtgctgtt tgccgtggtg tggatgcagg gggaatttga ttttggcggt acgccggtaa
     2761 atcacgcagc acagtttggt gcgctggttg ataaattccg tgcagacctg gcggatatgg
     2821 caggtcagtg cgtcggtggc tctgctggcg gtgttccctg gatatgcggg gacacgacgt
     2881 atttctggaa gcagaagaac gaatccacgt accagacggt gtacggcagc tacaaaaaca
     2941 aaacggaaaa gaatatccat ttcgtaccgt tcatgacgga tgagaacggg gtgaatgtgc
     3001 cgacgaacaa accggaagaa gacccggaca ttccgggtat cggatattac ggttcgaaat
     3061 ggcgtgacag ctcagccacc tggacgtcac aggacagggc gagccatttc agttcatggg
     3121 ctcgccgcgg gattatttcc gaccgtctgg caacggcgat tttgcgccat gcgggaagag
     3181 tggcgctaaa cgcgggggca tcatcgacag tatcagaggt gcgcccgtca tcgccttccg
     3241 gtgcagaagc cacaggcgtc acaacactgc tctcttacct tgccagcgag tcagagggaa
     3301 gcctgaaagt acagggatgg tcagccagtg gcggcagggc agaagtggtc agcgatgcgg
     3361 agggaaccgg aggtaaggca gtgaagctga ccaaggaagc cggtaaaagc agctgggtgc
     3421 tggagtacgc cgcgggcaac ggtgcggctc tgttacagaa aggggggcag attcgctgcc
     3481 gctttaaggt ttcgggagcg ctggctgcga accagtatgt tatggcgttt tactggccgg
     3541 tatcttcact gccacagggc gttgccctga ccggagacgg ggggaataac ctgctggcag
     3601 cgttctacat ccagacagat gcaaaagacc tgaatgtgat gtaccacaat gcgaaagtag
     3661 cgacaaacaa cctgaaactg ggaacctttg gcgcatttga taacgaatgg catatgctgg
     3721 ctttccgctt tgccgggaat aacagccttc aggtgacgcc ggttattgat ggtcaggatg
     3781 gcacaccgtt cacgctgacg cagtcaccgg tcagtgcatt tgcggcggat aaactgcatg
     3841 tgacagacat taccaggaat gcgacttacc cggtgctgat tgacagcatt gcggtggaag
     3901 tgaacaacgc ggatgccgcg gcatgataaa aaaagccgcc agttgcctga gacctctggc
     3961 ggtgtgagat tcatggagaa tcaaggaaag atactaccac tttcgtcatc ggcattttta
     4021 aatgaaaact gtttact
//
LOCUS       DAZFTN010000238         4017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFTN010000238 DAZFTN010000000
VERSION     DAZFTN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..631
                     /gene="espA"
                     /locus_tag="ACHKV2_005068"
     CDS             53..631
                     /gene="espA"
                     /locus_tag="ACHKV2_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO6942762.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            644..1768
                     /gene="espD"
                     /locus_tag="ACHKV2_005069"
     CDS             644..1768
                     /gene="espD"
                     /locus_tag="ACHKV2_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFO6942763.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1789..2727
                     /gene="espB"
                     /locus_tag="ACHKV2_005070"
     CDS             1789..2727
                     /gene="espB"
                     /locus_tag="ACHKV2_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO6942764.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2734..3141
                     /gene="cesD2"
                     /locus_tag="ACHKV2_005071"
     CDS             2734..3141
                     /gene="cesD2"
                     /locus_tag="ACHKV2_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO6942765.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3177..3398
                     /gene="escF"
                     /locus_tag="ACHKV2_005072"
     CDS             3177..3398
                     /gene="escF"
                     /locus_tag="ACHKV2_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO6942766.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3404..3682
                     /gene="escG"
                     /locus_tag="ACHKV2_005073"
     CDS             3404..3682
                     /gene="escG"
                     /locus_tag="ACHKV2_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFO6942767.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALEDCMDDETSQLLYSLVHENGSGWVRGF"
     gene            3767..>4017
                     /locus_tag="ACHKV2_005074"
     CDS             3767..>4017
                     /locus_tag="ACHKV2_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO6942768.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSRPAPPPPTSG"
BASE COUNT         1169 a          758 c          974 g         1116 t
ORIGIN      
        1 ttactcaata atttttttgt tttcctgaga aaaattatca agaggtatat agatggatac
       61 atcaaatgca acatccgttg ttaatgtgag tgcgagttct tcgacatcga cgatctatga
      121 cttaggtaat atgtcgaagg atgaggtggt taagctattt gaggaactcg gtgtttttca
      181 ggctgcgatt ctcatgtttt cttatatgta tcaggcacaa agtaatctgt cgattgcaaa
      241 gtttgctgat atgaatgagg catctaaagc gtcaaccacg gcacaaaaga tggctaatct
      301 tgtggatgcc aaaattgctg atgttcagag tagcactgat aagaatgcga aagccaaact
      361 tcctcaagac gtgattgact atataaacga tccacgtaat gacataagtg taactggtat
      421 tcgtgatctt agtggtgatt taagcgctgg tgatctgcaa acagtgaagg cggctatttc
      481 agctaaagcg aataacctga caacggtagt gaataatagc cagctcgaaa ttcagcaaat
      541 gtcgaataca ttaaatctct taacgagtgc acgttctgat gtgcaatctc tacaatatag
      601 aactatttca gcaatatccc ttggtaaata accggagata actatgctta acgtaaataa
      661 cgataccctg tctgtaacgt ctggggttaa taccgcctcg ggtacttctg gtattactca
      721 atctgaaacg ggtttatcgc tggatttaca actggttaaa tccatgaact cgtcagcagg
      781 ctggacagaa agtagccctt taccgacgcc gccggcaggt cactcattag tgacgccctc
      841 tgctgctgag gatgtcctta gtaaattgtt tggtggtatt agtggtgagg ttacaagtcg
      901 cactgaggag gcagagccac agcgcacaag ctatccctat ctctctcagg tgaataccgt
      961 tgaccctcag caaatgatga tgatggtcac tctgttatcc ctggatactt ccgcgcagaa
     1021 agtctcgagt ctgaaaaact ctaacgagat ttatatggat gggcaaacta aagcgctgga
     1081 gaataaaacg caggagtata aaaaacagct cgaagaacaa cagaaagccg aagagaaatc
     1141 acaaaaaagt aaaattgttg gccaggtctt tggttggttg ggcgtcgcat taacagccgt
     1201 tgccgctgtt tttaacccag cactctgggc tgttgttgcc attggtgcaa cagcaatggc
     1261 actgcaaacg gcagtcgatg taatggggga aaatgcccct cagggattaa agactgcagc
     1321 acaggtcttt ggcggaatat ctatggccgc aagcattctg acagccggcg ttggcggggt
     1381 gtcttcactg ttatctaaat ttggtaatgt tgctaacaaa attggctcaa gcgttgtaaa
     1441 agtcgttgag aaggcggcag aagcgctggt taaaaacgtt tttgcaaaaa tttcgacagt
     1501 ggctgagggc gttacgaacg gtattcgttc tgccgggaca actgcgttga ataatgaggc
     1561 tgcgcaactc caaatgttgt ctcagttagc tgctttcgcg gtgcaaaact taactcgaca
     1621 gagtgaaagc ttaggtgaga gtgcgaagct cgagctggat aaagcggcaa gcgagttaca
     1681 aaatcaggcg agctatttac aaagtgtttc tcaactgatg tccgattcag cacgggtaaa
     1741 tagtcgtatt gttagtggcc gaatttaatt attaaagaga attttattat gaatactatt
     1801 gataatactc aagtaacgat ggttaattcc gcttcggaga gtacgaccgg cgcttccagt
     1861 gcagttgccg catctgcttt atcaattgat tcatctctgc ttactgatgg taaggttgat
     1921 atttgtaagc tgatgctgga aattcaaaaa ctcctcggca agatggtgac tctattgcag
     1981 gattaccaac aaaaacaatt ggcgcaaagc tatcagattc agcaggccgt ttttgagagc
     2041 cagaataaag ctattgagga aaaaaaagcc gcggcaaccg ctgctttggt tggcgggatt
     2101 atttcatcag cattggggat cttaggttct tttgcagcaa tgaacaacgc ggctaaaggg
     2161 gctggtgaga ttgctgaaaa agcaagctct gcatcttcaa aggctgctgg tgcggcttct
     2221 gaggttgcaa ataaagctct ggtcaaggct acggaaagtg ttgctgatgt cgcagaggag
     2281 gcatccagtg cgatgcagaa agcgatggcc acaacaacga aagcagccag ccgtgcatct
     2341 ggcgttgcag atgatgttgc gaaagcctct gactttgctg aagatcttgc agacgccgcc
     2401 gagaagacaa gcagaatcaa taagttgttg aattccgtag ataaactgac caataccaca
     2461 gcatttgttg ccgtgaccag tcttgctgaa ggtacgaaaa cgttgccaac aacaatatct
     2521 gagtccgtca aatcgactca tgaggttaat gaacaacgtg cgaagtcgct ggaaaacttc
     2581 cagcagggga atctggagct gtataaacaa gacgttcgca gaacgcagga tgatatcacg
     2641 actcgtctgc gtgatataac gtccgctgtc cgcgatctcc ttgaggtcca gaatcgtatg
     2701 gggcaatcgg gtcgcttagc tgggtaattg atcatggtcg atacgtttaa tgatgaagtg
     2761 tttaattact atcttgaaca aaaagggtac acaatacaga aggagtttct ttgtggcagt
     2821 gcctttttta tcggatggcg gattgagaca cctttttttt cattagcgta cagactggat
     2881 gaacaagaac tgattttgtg ctcttttgaa gcacgtaacc aaacagggct taacggccct
     2941 gttttatcgc tgactcattt gctcgaagag ttgtatcacc atttttcggg tattaagaaa
     3001 atcagcgcga tgaaatctaa gattggttca gattcagaac gtcaaaagcg cgaagagttg
     3061 tttaattact tcatcagaaa gggtgccgtg cagcaagaaa cagaagacgg aatttggttc
     3121 gtaatgaacg taaatagtta aaagttttta ttttattaac ttctgaggga aatttaatga
     3181 atttatctga aattactcaa caaatgggtg aagtaggtaa aacgctgagc gattctgtgc
     3241 cagagttact taatagcacc gatttggtta atgaccctga aaaaatgtta gagttgcagt
     3301 ttgcggttca gcaatattct gcttatgtta acgtagaaag tggaatgttg aaaacgataa
     3361 aagatctggt ctcaaccatt tctaaccgta gtttttaatt ttaatggtta atgatatttc
     3421 tgctaataag atactggtgt gggcggctgt agctgcggca aaccataagc ttcccaaata
     3481 tgcggaagct atcctgaatg tattcccaca aattatacct gataaaaaag atatcgcaca
     3541 tttagaattt attatcttat ttggattaaa tagaaaaaat gatgcggtaa aggctttgga
     3601 ggactgtatg gatgatgaga cgagtcagtt attgtacagt ttggtccatg agaatggtag
     3661 tggctgggta cgaggatttt aatttatatt gatttaaaaa tcattttatt attcgttttg
     3721 atatatatga gagttagcca agcttagata taaagaggca taaattatgc ttaatggaat
     3781 tagtaacgct gcttctacac tagggcggca gcttgtaggt atcgcaagtc gagtgagctc
     3841 tgcgggggga actggatttt ctgtagcccc tcaggccgtg cgtcttactc cggtgaaagt
     3901 tcattcccct ttttctccag gctcgtcgaa tgttaatgcg agaacgattt ttaatgtgag
     3961 cagccaggtg acttcattta ctccctctcg tccggcaccg ccgccaccga caagtgg
//
LOCUS       DAZFTN010000239         3727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFTN010000239 DAZFTN010000000
VERSION     DAZFTN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..825
                     /locus_tag="ACHKV2_005075"
     CDS             <1..825
                     /locus_tag="ACHKV2_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6942769.1"
                     /translation="SDWVEVGPFGNGEKLKVKIESLGKTSDTIGKSSNAQAVLPYVVK
                     IARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLTSKLA
                     GDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLKCDNL
                     FGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKISAN
                     GDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALEATALINVKYD"
     gene            complement(907..1245)
                     /gene="rcnB"
                     /locus_tag="ACHKV2_005076"
     CDS             complement(907..1245)
                     /gene="rcnB"
                     /locus_tag="ACHKV2_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO6942770.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1463..2314)
                     /gene="rcnA"
                     /locus_tag="ACHKV2_005077"
     CDS             complement(1463..2314)
                     /gene="rcnA"
                     /locus_tag="ACHKV2_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO6942771.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHEHHHHHEHGDNEEYQD
                     AHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATL
                     VVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVH
                     GFMGIMR"
     gene            2435..2707
                     /gene="rcnR"
                     /locus_tag="ACHKV2_005078"
     CDS             2435..2707
                     /gene="rcnR"
                     /locus_tag="ACHKV2_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO6942772.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2817..3131)
                     /locus_tag="ACHKV2_005079"
     CDS             complement(2817..3131)
                     /locus_tag="ACHKV2_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO6942773.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3141..3488)
                     /locus_tag="ACHKV2_005080"
     CDS             complement(3141..3488)
                     /locus_tag="ACHKV2_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942774.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1057 a          764 c          862 g         1044 t
ORIGIN      
        1 agtgattggg ttgaagttgg gccttttggt aatggcgaaa aattaaaggt taaaatagag
       61 tctttaggta aaaccagcga cacaattggg aaatccagca atgcgcaggc agtattacct
      121 tatgtggtta aaatagccag aggcacacct gattttactg gagaaagaaa atctacctgg
      181 tttatttcag ataccgtgat tgcaaatatt ggcggtgagt catcgtcatc catcgatttt
      241 tggttgggta tttgtaaggc attgaagttt aactggtgtg tgaattatct caccagcaaa
      301 ctggcggggg atacatttac gcttgggtta aatatttcct attatcctaa aaatacgacc
      361 tgtaagcctg aaaacaccgt tataaaagta gatgatatcg ccttgttcca gctcagaaat
      421 cagggaaaga ttgcggcgaa cagtaaggaa ggaacaatta cgttgaaatg tgataatctt
      481 ttcggcgaca aaaaacaagc atcgcggaat atggttgtat atctttctag cagtgactta
      541 gttaaaggaa gtaatactat tttgcgtggt aaaacagata atggtgtagg gtttgtgttg
      601 gatctaacag aaccaccaaa agggactgag gctgccatta aaatttcggc caacggcgat
      661 cagggcgcgg cgacatcatt atggaaaaca gataaaccag gagtttcatt aaatagcaac
      721 attattaata taccagtcat ggccagttac tatgtatatg atgaaaaaaa agttaaatct
      781 ggcgcactgg aagcaaccgc attaatcaac gtgaaatacg attaataaca agcaagatga
      841 gcaagtggct aataataaga aataataagg tgtctgcatt tccccctctc tccgaggggg
      901 ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt taccgtcggt
      961 gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg catccggcgc
     1021 gggcaggtta cgcaactgcc actgcttaat gttgtactca tcggtacggt acatctctgg
     1081 tacggtgtcc cctggtttgt aatgcttaaa gtcagcaatg aaactgctta actcatactt
     1141 agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg cggcactggt
     1201 aaccagcaaa agcgcaccca gcaacatctt atttttaatg gtcataaact ctccactttg
     1261 gggatctgta atatcagtcg catatctttc ctgccataca atataactga caagcgatat
     1321 tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgtag caaaagcatc
     1381 ctcattgagc gagaagtgag cggaataagc tgatttaaga atcatcctgg gctaaaagga
     1441 tagcgaagcc caggtgattg ttttatctca taatacccat aaagccatgc actcccatat
     1501 acaccccgac taaaccaatt aacagactgg aaaaataggg ggcgcgttta gcgagagtgt
     1561 taaatccgct ccagcgtttt gcaacttgtt gaacactgat tgccgctcca acgcctactg
     1621 taaccagcgt tagtgccaga ccgatgctga aactgacgac cagtgttgcg cccagtgtca
     1681 gggctttcaa ctgaatgcaa atcaacagca cggtaattgc tgccgggcag ggaataaggc
     1741 cacctgttaa gccaaataac aaaatttgcc agttggtgac ctctctacca tcaaagcgtc
     1801 gtttaatgtc attggcatgg gctcgtgcat gggcatcctg atactcttcg ttgtcgccat
     1861 gttcatgatg gtgatgatgt tcgtggtcgt ggtcgtggtc gtggtcgtgg tcgtggtctt
     1921 gatgatgttc gtgatcgtgg tgatgatgct catgcatatt ctccagccag ttgcgttcgc
     1981 cgcgccaggt acgccagaac atccagaaag cggtgctaat aatgatcact gcggaaatca
     2041 actggagcca cggttctgct gattgagcag taaagcgctt gctgatcacc atcccgccaa
     2101 aggcaatcaa ccagaccact gcggtatgcg aaatggttgc tgccagtccg agcatcaccg
     2161 cctgtttaat ggtgcctttg atggcgatga taaacgccgc catcatcgtt tttgagtgcc
     2221 ctggttccag gccatgaagc gcaccaagta agatggcgct ggggatgaag aaccaggcgt
     2281 ttccttgctg aagaagagtt gtaaattcgg tcatgataat aattcttagt attaattcgg
     2341 caatctgatt ctactccccc ccagcacctg atactacccc ccagtagatt aatagtgcta
     2401 tgatttttca tgttcttgtt aaccaggtgt tgccatgtct catacaatcc gtgataaaca
     2461 gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca agaaaatgct
     2521 cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc gtggcgcggt
     2581 aaacggtctg atgcgggaag tgattaaagg tcatctaacg gaacatatcg ttcaccaggg
     2641 ggatgagcta aaacgtgaag aagatctgga tgttgttctg aaggtgctgg attcttatat
     2701 caaatgaaag tgcctaatat ggccgtagtt gaggcaataa atagagggac aagtgacgtt
     2761 aagctgaatt agagtaaatg gcgtgaggac acacgccatt cgaggttata gtttgattaa
     2821 ttttcgaaaa attgcttcac agcccagcca ccatgaccac ctggagttat gaatgaaaca
     2881 aaacctttgt catctacgct ctggatttct accgtcattg attcatctaa cacgcgacat
     2941 ttattggatg taatgagact cagcatagcc tgttggtcat tgtcctggat gtaactgatc
     3001 atctcgtaaa agtcagttaa ctttttacag ttaaatgtac cttctttaag tgttgcgctg
     3061 tctccgtgac ttagtgcgaa tgcatttggc atcattgcca caaagatgac ggtaacgagt
     3121 agttttttca ttttagcctc ttataattca gaacattcac tgatgttgta agtgcctttt
     3181 ctaccatcat cgtttacgag atgtagcacg aagatgatgg gattggcatc aatgaaagat
     3241 acagagacgt tcccgctttc ggtgctatac atctttgcaa tctgtttttc accaatatat
     3301 gagagatccc aactcactcc gttcaagcta aaatgcatct gtttgccgtc aatatcgatg
     3361 gaggattgtg ccgtaacggt tttcccattg ctgccaattt gaccctgctg aagagcatca
     3421 catttatagg agcttgcata agtagataga ggtagtagca gaagtgcagc aaaaatttta
     3481 ctcttcatat tttccctttg cttggatttg ggttacctat tttggggtag tcattataaa
     3541 actattaatt agtaaaacga acaagttttc atttgtttag caatggtatt gtttaggttt
     3601 gaaattatta cgtattttca tagcaatagg ttggataaac agcagttcag ggaacaagtt
     3661 gcactatcta catacttttc cgctttccat tgcatgttag gtaatagata gcctttgccc
     3721 gtaagac
//
LOCUS       DAZFTN010000240         3718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFTN010000240 DAZFTN010000000
VERSION     DAZFTN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1156)
                     /gene="rhsD"
                     /locus_tag="ACHKV2_005081"
     CDS             complement(<1..1156)
                     /gene="rhsD"
                     /locus_tag="ACHKV2_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO6942775.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLPGAPLARYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSY"
     gene            complement(1223..3331)
                     /locus_tag="ACHKV2_005082"
     CDS             complement(1223..3331)
                     /locus_tag="ACHKV2_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6942776.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          769 a         1127 c          978 g          844 t
ORIGIN      
        1 ggtaactcag gcctgccggg ttcagctgct ccaccacccg cccggtatcg tcgtagcggt
       61 agcgcatctc cggccttccc gcgtaacggt gcgccaccat ccggcccgga tgctgcgcgt
      121 catacgtgaa agcgcgcacc tgcgtattgc tgcggtcata taccgccagc agttcaccgg
      181 cttccgtata cgtgtaccgc gccagtggcg caccgggcag gctttccggg tatgccgggt
      241 cgtgcgtcag ccacaccgcc gaaaggcgga tacctctgtc gggaccgtat tcggtaccgg
      301 gcagtgtgtc ggggaacgct gagtctgaaa gagggcgggg ggtgtcaggg gaagataaag
      361 aagcggtgtg ctgtttacgg gcctcttccg cccgctgcgc ctgcgtggtc agcaccagac
      421 ggaactcccg cccggcaccg tccgtcacgc cggtgatttc cccggccagg tcaccggcgg
      481 cctcacgcct gtacgtcagc gtccgcccga agcggtccgc catcccggta agcacccggt
      541 acggcggcag cggcgctggc agtacgtcct cagcacccgg cacccgctct gaccacccca
      601 gtatccacca cggcccctgt gcgctgttgg tcgccaggta aagatgcggg cttaaccgga
      661 tatccggcgg cagcgccccc cacagccgcg ccagcgtgtg gccgtccggc tgcgctgcct
      721 taccaccgcg caccagccac attgactcgc tgcggctgta caccgcctcc cccggcagca
      781 gcggctcaaa gtgaatgctc cgcccgccgt tgtcattgag taccagtgca tcatcgcgta
      841 gctgtaagcg gatatcagaa ggcgctttcc agccggggcc aaaaatcccc accggcgcag
      901 gcgtccgggt ccggtagctg ctgtaggtgc gggagagaat gaacggcagc gggccgggca
      961 gcgcaaggtc cgtctcgccg ggcagcacct tcgcccccag cagcggattt accgggttgc
     1021 ccgaagtcat cccgcccggg cacaccgagc acgccacgcc agttggcgcg ccaattctta
     1081 cacctgccga accctggaca atgggaccgc catactgagt catatctccc tgacgcgccg
     1141 ctggttttcc gctcatgatt caattccttc ttctgtttat ttttaatgct tgtttgttac
     1201 tgaataccga acaggtacgg gatcagtact ctctcatatc cggcgtatcc tgcttttcat
     1261 caggggtctt cggtggggat ttcgaggctg cacagttcca gttaatcaac agtgagtcac
     1321 cattaacctg ctccttaccc tgcaaatgaa tttttgtgcc gttgagaaaa atttgtccgt
     1381 ccttcgtcag caccagcctt gccttaccac agcagagctc cagatgctca ccggcggagt
     1441 aaatcgcggt ctgcccggta tcatcctttt tcgttttacc aacggtgaag gtaacgttat
     1501 ttccgacttt gacgtcataa cccagcccga ccctcaacga tttatgcaaa ccgatctgcg
     1561 aggactgcat cagagccacc gaagtgttca taatgccacc taccgtcgtc tggtacgcca
     1621 cgcccacggt cagcgcacga acaagtccaa tcgtttccac ctgaacagaa cccacgttaa
     1681 tgatctggtt actgcccacc gtctctatct ggttaacgcc caccgtctgt atctgattgt
     1741 tggtaaccgc aatcatctga ttgttgccaa tggtctcagc atggttgttt atcacatccg
     1801 tggtgcggtt attcagcacc tcggtgttca tgttcttctg cgcgtggatg tagacctgtt
     1861 ctttccctgt cgcatcgtca aacttcagtt cattaaaccc gctgccctta tagtttttcg
     1921 aacgaatggt catctgcgtc ttcgtccccg gcaggctgcc gggtgtgcgg ttttcctggt
     1981 ggtaggtacg ccccataata atcggctggt ccggatcgcc gttgaggaag tccacaatca
     2041 cctcctgacc cacacgcggt atcgccaggt tgccaaagcc ggtgcctgcc cacgcctgtg
     2101 ccacacggat ccagcatgaa ctgtcctggt ttgacgggtt atagcggtcc cagttaaatt
     2161 tcacccgcac gcgaccatgt tcgtcgcaga agatttcctc gcctgccggt cccgtcacga
     2221 cggcgctctg cgggccatcc accagcggtt tcagcaacgg ctgtggtcgc catgtccggt
     2281 ctgccggtat taccgcaaag tggttattca gcgtggtacc tgaacccctg cgtcccggca
     2341 ccgcctgtgg ctgctcgccg tgcagttcac ttgccaccac ctgccattcc cggttcaggt
     2401 tcgcctgcgg atgccccgtc agcacaattc gccgtcccgg ccatatttcc ggagaacggc
     2461 ttgttccgcg cgccacttct gcgttgttgc gccagccatc catctgccag cgggcaaagt
     2521 tctgcccgtg ggcacccttg aaacgtccgg ggtagtcata cacttcatac tgtgtgcgct
     2581 ggtaatcctg gtactggcct tcctgatcaa aacgccctgc ccagccgggt cgtttaaagg
     2641 tgtagtcctt ggtcaccacg gaagaagggc ggatttgtgc gctgtagcgg aactggctga
     2701 tgcatagggt gctcacctcg gtacgggtgt tcgggttcca ggggatctca aaggactccg
     2761 gcagataacg cacggtgtcg cacaggacca ggctctggtc gatacttttt tgcgcgtgct
     2821 cctcataaaa gaagatgcct tcctccgccg ccatccggca caggaaatcg taatcagttt
     2881 cgccgtactg gacacaaaac tcacgggaag gatgcggctc gctgaacagc ggactccact
     2941 cggtcacacc gttttctttc aggatcgtag cgagtatgct ttcgatgtcc tcattctgga
     3001 agatacggaa gttctggcgc agccctgtgc gccacagcgg cgggcacacc ttcatgctgt
     3061 acagcatctg gtttttgtcg ttctccccca gttcaaacca ggtcaccaca cctttcaccc
     3121 ggcgctgtac gtcatcgccc tgccataccg tcaggtaggc cattttgtcc agcacctgcg
     3181 caaattcaag ggaaagaaac tgctgactga ccagagaaag gtcgagggaa aaaagtgaag
     3241 agagtgactg gttcagatga aaggagacca ccgcaaacgc gtccggtggc aggccgtcca
     3301 cttccagcgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
     3361 cgcacatatt gcatgtacaa catgtcgggc ggatgaaata agtgagtcga catctggcgt
     3421 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
     3481 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
     3541 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
     3601 tcaaaccaat attcataatg tctttttatt cacaacaata aataatttaa taacaatcat
     3661 tgaaatgaat gatgatttgt ataaggaata tacgctaccg ccccaccctg ataacatg
//
LOCUS       DAZFTN010000241         3680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFTN010000241 DAZFTN010000000
VERSION     DAZFTN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..233)
                     /gene="escO"
                     /locus_tag="ACHKV2_005083"
     CDS             complement(<1..233)
                     /gene="escO"
                     /locus_tag="ACHKV2_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO6942777.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDS"
     gene            complement(293..1633)
                     /gene="escN"
                     /locus_tag="ACHKV2_005084"
     CDS             complement(293..1633)
                     /gene="escN"
                     /locus_tag="ACHKV2_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO6942778.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1617..3644)
                     /gene="escV"
                     /locus_tag="ACHKV2_005085"
     CDS             complement(1617..3644)
                     /gene="escV"
                     /locus_tag="ACHKV2_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO6942779.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT         1118 a          863 c          676 g         1023 t
ORIGIN      
        1 gaatctagca atgaacgctt ttccttcgaa agtgaagatt ttttctcata caatcgctgc
       61 ttttgctcat caaatgcatt gtttttaatc tgatatttat acaacgacac tttatccaaa
      121 ttcaccagcg atgcacaata tgcctgctta gaggcaatcg actctttaat ggattgctcg
      181 cagtcatcga gtttaccatc cacctctttt atctgagagt taatcttagc cattgattct
      241 ctcaatcggt tcgctctgct tttacgaata gataaaattc tgtccaacat actcaggcaa
      301 ccactttgaa taggctttca atcgtttttt cgtaactact gatatctttg gtgctttgtt
      361 gtataaagtt ctgaattaat ttcctatttt ttattgcctt atctgcttca ggatcttgcc
      421 ccatcgtgta ctcaccaata cgaataagca gctcaacatt tttataagtt gcaataagct
      481 ttttgcattc tgccgccgca cgcaaatgct ccgatgtaac aacgttatgc ataacacgac
      541 tggcacttaa accaatatca attgcaggga aatggttttc ctctgcaagt tctcgggtaa
      601 gtacgatgtg cccatcaaga atagagcgga cttcatcgcc aataggatca ttaacattat
      661 cgctttctaa caacaccgta taaatcgcgg ttattgaacc ttttggcgca ggccccgctc
      721 gctctaataa tttgggtaac gaagaaaaaa cactcggagg gaatcccccc cttacatcag
      781 gttctcccga tgcaagccca acatcacgtg cggctcgggc ataacgagtt acagaatcca
      841 tcattaataa tacattttta ccttgatcgc ggaagtactc tgcaatagtc gtcgccgtaa
      901 atgcggcttt catcctttcc agcgcggggc ggtctgacgt tgtgacgacc agtacacatt
      961 tagaaagcgt ggattgaggt aagagcgcga ggaattcatt tacttcgcga ccacgttcgc
     1021 cgataagagc aaggacaata atatctgcgg atgcgccatt acaaatcatc cccaaaagcg
     1081 tacttttacc aacgcctgaa ccagcaaaaa taccgatacg ctgcccaata ccgcaagtta
     1141 gtagcccatc aattgccctg acgccaagta taaatggctg atcaataacc tgccttaata
     1201 aaggatctgg tggttcagca tacaagctgc gttcaaacgg aagatagggg gcagcaatat
     1261 tactctccat tggtctgcct atgccatcaa caagccgccc aagtagtgcg tctccaacac
     1321 gaatcttgaa ctcatctccc tgataactta accactgacc acagtacatg ccggaaacat
     1381 gctcaaaagg caacaagaaa acctcatctt catcaatagc gatcacctcg gctaaacgtt
     1441 gactgggttc tatcttataa aaggcgccaa tacgcgcttt tggtaaacgc gccttaataa
     1501 tcgtcccgcc gatattgata atcttgcctt catatctggt agacgaattt aatgataatg
     1561 ccggcacagt gctattgagc actttctgaa tacgtgggta tttttccaat acagaatcat
     1621 gctctgaaat catttaccgt tcctaatact ttaagttcga tattattccc aacctcctga
     1681 aaagagagca cggggactga cgggaacgaa ccttcaatca ttttcttcac atagcgcctg
     1741 atatctaaag cggtcagaat gacgccgttc cccgttggat tcatgatatt ttttaagaag
     1801 ccaattatct gctcagttcg ctccggtgag atattcagat acgaacctga tgacgtttgt
     1861 ctgatcgact ctcgtagctc attttcaata tcagagccaa taagccaaac gttcagaagt
     1921 gtaccgctaa cgctatagcg acctaaaata tgccgacgca gggcaatacg aacgtattcg
     1981 cacaagataa ccacatcctt ttcttttgtt gaccaaaaaa taagcgtctc aaagatagtt
     2041 ctcaggtctc taattgagac attttcctct acgagacgtt gtaggatgtc aacaattttg
     2101 ctcaaaccaa gctggcgctg cagctctttc acaagctcgt tatattttct ctccatgatg
     2161 tccatcaaat aacgcgtttc ttgtacgccg atgaactctt tggcattaga caatacaagt
     2221 ttctttagca aaaacgttat cttatcattt gttgttgcat aaacatccat acctgtaagg
     2281 tgagcgagct gttgattagt cttattcgtc ttatatacga tagagttagt cgaaagcttt
     2341 tcctggacaa attcgaatga tatctctgca tgcccagcct caaaatagac agtatcatct
     2401 ataagagtat ctttgtatat tgactcttga tagagcaaaa attctattgc gctatcatta
     2461 tttttaaccg gattaacaat aatcttaggc aatggaattc cactttcctc aaagaaattc
     2521 caacgcatga cctcaatttg ctgtgtaatg tctgaactat aaatattact acttaagttt
     2581 aaaatcaatg gctcagcgcc cggtgagatg ttttggctat tagacaaatc agctcccata
     2641 gcatccttat tattgccagt tccaaatgtt cttttcttct gtaatttcca ggctatcccc
     2701 aacaggcaaa ccgccagaaa taagaagacc aatgcaggaa agcccggaat aatcgccatt
     2761 acgagcatta gtgcggcact gattaaaaaa gggcgactat taacagaaac ctgttgaaca
     2821 atatcacctg caaggttttc ttctttttcg ctttcaccgg gcacacgagt aacaaccacg
     2881 cctgcggtga cagaaataat aagcgcaggg atctgggcaa ccaaagcatc accgacagac
     2941 aatacagaaa acaggctaag tgccgcacta aatggcatgt caaattgcca cataccaatg
     3001 agcacgccac caaaaaggtt aactaaaaca ataatgatac cagcaatagc gtcccctttt
     3061 acaaacttca tcgccccgtc cattgaacca taaaactggc tttccttttg cactcgggag
     3121 cgaagaactt ttgcctcaag cgggtcaata acgcctgcgc gcatatcacc atcaatactc
     3181 atttgctttc ctggcatacc atcaagggaa aaacgagcac tcacttcagc aacacgctcg
     3241 gccccttttg taataaccat aaattgaaca atggtgataa tagtaaaaat aaccaggcca
     3301 acaacaatat taccgccaac aacaaagtta ccgaaggagt aaataatgtc acccgcatcg
     3361 tgatgcagca gaataagtct ggttgtacta acactgagcg acaggcgcat caacgtcgta
     3421 atcaacagga ttgtggggaa agaagttagt tcaagaggat tttttatata gattgagagc
     3481 attaataaaa gtaaagctgt cgaaatatta atcgcgataa tgatatccac cacaactgtt
     3541 ggtagtggaa taatcatcat cattacagcc ataaagaaga agagagccag aataagatcg
     3601 tgatatgact ctgctttttt aaatatattt aagagtttat tcatgatgtc atcctgcgaa
     3661 cgcgctcaat aatctgcaca
//
LOCUS       DAZFTN010000242         3674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFTN010000242 DAZFTN010000000
VERSION     DAZFTN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..224
                     /locus_tag="ACHKV2_005086"
     CDS             <1..224
                     /locus_tag="ACHKV2_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6942780.1"
                     /translation="DQEDVALPFPELLANVGDEVTPVDVVAERAGQPVPEVVTQLLEL
                     ELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            196..669
                     /gene="smg"
                     /locus_tag="ACHKV2_005087"
     CDS             196..669
                     /gene="smg"
                     /locus_tag="ACHKV2_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO6942781.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            698..1240
                     /locus_tag="ACHKV2_005088"
     CDS             698..1240
                     /locus_tag="ACHKV2_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO6942782.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            1245..1817
                     /gene="tsaC"
                     /locus_tag="ACHKV2_005089"
     CDS             1245..1817
                     /gene="tsaC"
                     /locus_tag="ACHKV2_005089"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO6942783.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            1822..2640
                     /gene="aroE"
                     /locus_tag="ACHKV2_005090"
     CDS             1822..2640
                     /gene="aroE"
                     /locus_tag="ACHKV2_005090"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO6942784.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            2637..2894
                     /locus_tag="ACHKV2_005091"
     CDS             2637..2894
                     /locus_tag="ACHKV2_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO6942785.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(2870..3424)
                     /locus_tag="ACHKV2_005092"
     CDS             complement(2870..3424)
                     /locus_tag="ACHKV2_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO6942786.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT          941 a          851 c          933 g          949 t
ORIGIN      
        1 cagatcagga agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg
       61 taacacctgt tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc
      121 aactactcga actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat
      181 tgaggagggc atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca
      241 gaagctgagt tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt
      301 gatcgagaag atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa
      361 ggactggcag aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct
      421 gaggagtgtg aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcaaatt
      481 caggtgctta accttgaaac ccgtgaaatg gtgatagagc gagtgctcgc gctggatacc
      541 gcagagttcg acctggaaga cctgaaatgg gtaatcctga tggtgttgtt caatattccg
      601 ggctgcgaaa atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg
      661 ctgcattaat catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac
      721 ggtgcgtaat aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa
      781 acacggtccg tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa
      841 atcttcagcg gatggacata tcgtcaaagt tctggagggg caggtttgcc ctgcatgtgg
      901 cgcaaatctg gtattacgcc agggacgctt tggtatgttt attggttgca ttaactaccc
      961 tgaatgcgaa cataccgaac ttatcgataa accggacgaa acagcaatta catgtcccca
     1021 atgccggacg ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg
     1081 tgatcgctac ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc
     1141 tgagtgtcat tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg
     1201 tgccagtaaa caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc
     1261 aaggagacgc tatcgcagct gcgatagatg ttctcaatga agaacgtgtc atcgcctatc
     1321 caacggaagc cgttttcggt gttgggtgcg atcctgatag cgaaacagca gtgatgcgac
     1381 tgttggagtt aaaacagcgt ccggttgata aggggctgat tttaatcgca gcaaattacg
     1441 agcagcttaa accctatatt gatgacacca tgttgactga cgtgcagcgt gaaaccattt
     1501 tttcccgctg gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct
     1561 ggttgacggg ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg
     1621 ctttgtgcca ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc
     1681 caccttgtcg aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc
     1741 ctggtgaaac gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac
     1801 tgtttcgaca ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac
     1861 agcaaatcgc cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat
     1921 gggcgcgtgt tggcacccat caatgatttc gtcaacacac tgaacgcttt ctttagtgct
     1981 ggtggtaaag gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat
     2041 gagcttactg aacgcgcagc gttggctggt gctgttaaca ccctcaagcg gttagaagat
     2101 ggacgcttgc tgggtgacaa taccgatggt gtaggcttgt taagcgatct ggaacgtctg
     2161 tcttttatcc gtcctggttt acgtattctg ctcatcggcg caggtggagc atctcgcggc
     2221 gtactactgc cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc
     2281 cgagcggaag agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg
     2341 gacgaactgg aaggtcatga gtttgatctc attattaatg ccacatccag tggcatcagt
     2401 ggtgatattc cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg
     2461 ttctatcaga aaggaaaaac tccttttttg gcatggtgtg agcagcgagg ctcaaagcct
     2521 actgctgatg gtttaggaat gctggtggca caggcggctc atgccttcct tctttggcat
     2581 ggcgttctgc ctgatgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga
     2641 atcaggccat ccagtttccg gacagagaag agtgggtcga gaataaaaaa tgtgtatgtt
     2701 ttcccgctct cgtgaatggc atgcaactga catgtgcgat ctctggcgat agcctggcgt
     2761 atcgctttac aggagatacg ccagagcagt ggttagcgag ttttcgtcag catcgctggg
     2821 acttggaaga agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg
     2881 tctggttacc ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc
     2941 gtaacccagc cttctcttca tcacttaacg ggcggatctg tttgacggga ctaccgagat
     3001 acagatatcc gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca
     3061 catcatcttc tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat
     3121 tgccaatggt gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg
     3181 ttaatgggtt gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat
     3241 cctggatatt ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga
     3301 gcggccagat ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt
     3361 cgatcattac gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag
     3421 acatacttac ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt
     3481 gcaagtcttt tagtatgcaa aaaagcaccg ttttgtgtgc gaatgcagca aaaagggtga
     3541 aaaaacaaca aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta
     3601 taatgcgcct ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacgaa
     3661 gagaaaaaat cctg
//
LOCUS       DAZFTN010000243         3632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFTN010000243 DAZFTN010000000
VERSION     DAZFTN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(625..822)
                     /locus_tag="ACHKV2_005093"
     CDS             complement(625..822)
                     /locus_tag="ACHKV2_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942787.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(888..1158)
                     /locus_tag="ACHKV2_005094"
                     /pseudo
     CDS             complement(888..1158)
                     /locus_tag="ACHKV2_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1463..1651
                     /locus_tag="ACHKV2_005095"
     CDS             1463..1651
                     /locus_tag="ACHKV2_005095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942788.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1979..2173
                     /locus_tag="ACHKV2_005096"
     CDS             1979..2173
                     /locus_tag="ACHKV2_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942789.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2516..2644
                     /locus_tag="ACHKV2_005097"
     CDS             2516..2644
                     /locus_tag="ACHKV2_005097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942790.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2954..3289)
                     /locus_tag="ACHKV2_005098"
     CDS             complement(2954..3289)
                     /locus_tag="ACHKV2_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942791.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1103 a          803 c          788 g          938 t
ORIGIN      
        1 atcttctggt agagattcag gatacagttt gtaggagctt tcttttttga acaatgattt
       61 gcaggacagt tatgctaaac tagcggaatc agtatcacaa caataaatga ctgatcatac
      121 ctgaaatttg aattaacagg catcatgaat gtcaatagtc taagcggtga ctacttatat
      181 cgcttgcata aatactaaac actgactaga aaagtattgg ataaccccgt cactaaaagt
      241 aacgcttcgg ttatttgctc ggaatgtatt aacgccaaga ttgttagccg ttacgcctta
      301 tatcactggc gctgacaaat gtttcttttc taatttactg aaccggcgca gacacgcagg
      361 aaagcacgcc ggcaccggtg taatgccagt tctaatcaat aagaatggaa tgacgaatca
      421 gtcaggaagg gataacaacc cgctgctgaa cggaaggtat cagcaggaag atgaacggga
      481 atatcgccag caccaagggc agggccgggt tccacaatac cagcgctggc attttgcaca
      541 ttgccagcgt accggcatta caacacgttt catgctgctg ccccccagga ttcctcgatt
      601 cgttgctaca tccactgctc tacttcactt ccactgctcc acttcacttt tgtaccagcg
      661 gctgctgcgc cccagtttga tgggtttagg gaaatggcct tcactgatca acttgtaaaa
      721 ccatttgtcg gtcataccgg tgaaactggt gatatagttc atgtcgatca gggaattatc
      781 ggcacgcaga aatacgctgt cgtggcttgt tgtagtcaac atggtcagaa tgtcctgtga
      841 gatttatgaa gattgtgcga atgcgggaaa tctactgagc tgtgctttca gaactggcct
      901 gttacgggat accagggatt accggcgggg taacgggctt ccggatcata cacaccacga
      961 ttatcgcgga caaaatcact gaacgcccat atcacctctt taagtatgtc ttcgcagtcc
     1021 ggtaaatgac gatccagcgc cacatcccgg gtggtacagc tttgatgcgc ccggtgacac
     1081 aaagcccgga ttgttccaga catcctgaat caaacgcccc agattagggg cgtcgaaata
     1141 tgcctctctg accattatat tccggcgtat aggtagcagg tctgaagtga caatgcgtca
     1201 cctgacgtta aaagtcaccc aagatgaccc cagtgggggc aacaagtata aaaccgtttt
     1261 ttgattaagt ggtcggatat gaaaagggga aggtttatgt gaaaaatacc tgtgttttct
     1321 ttgaaaacac acaacagtat caggttattt atgtttatac agtgcgttcc aaactgacct
     1381 gaataattac gcaagttgca acaataaaac atttttgccg tagtgtgagg tactccggta
     1441 aattgcttcc caggtgcaga caatgactaa cgaagaacta caggcaatcg cattatccat
     1501 gcttgagcgc cagcgcgaaa aagtacacca gcaacgagag tctttgctta acccatctcc
     1561 attcaccagg ccaaaacgat tggagcttga agcattcttg gacaaatacc cacggcggct
     1621 taaatcaggc aaaaccagac caccaggctg atagctcaca gtttgttctc cgattcaata
     1681 aaacggataa gaaccatcag cgcggtagcg taaggcacta cagcgcattg ctttacgagt
     1741 aataacaatg gatttatcac cagaagacgc aaagttttta cgagaactgc aacaccgtat
     1801 gctcagagaa gccattatcg aacagcaaac gggcatgagc gccatagacc cattgcgata
     1861 caaacgggcc tcatctcctt tagttgacga caattccaat gcggataaaa cacgtccacc
     1921 acactgatta catttttcat tagcacagtg gtgtgatgca gcatacgagg aaaaccggat
     1981 gaatgagaac aaaataaaaa ggctggagca actgttacag gcacgccaac gggagtttgc
     2041 cactaaatgg ggacgtgccg cgctgccaca tgaacgccta acctattttc cacttgaaga
     2101 cctcattaaa aataatggca aaaaaatcag gtctgataag aaacacttct tagaatgcaa
     2161 tgaagacaaa taacaacaat ctgcatattg ccaccaacca cagatgtggc atcgttatca
     2221 gcgcacttga ttttctttat atccactgac gagtttgccg gtcattctgg tatcatgcag
     2281 atatttacgg cgtacaagca cactagcatg acattgtaat aaggcctgag cctaacgctt
     2341 taatgtgtgt catacactca gtggcgtaat cgttgacaat atggtgcatg taatcgtaag
     2401 cgtccagcga aatccatctg gtcataatct gaagcatccg acaaagtggt gtccaccaaa
     2461 taagtgatct gcgcccaata tcctgagccg gaactttgac aaaggccaac gaaaaatggg
     2521 agcggatgtt acagagttct cggtacaggg gaaaagtggt acctgtcgcc agttctccga
     2581 tctgtttaac cgggaaatta tctcctacag cttgtcagaa aggtcggtga tggagcatcg
     2641 ttaatacgat gctatacgat gcattcacag tgcccggcca gaggatgccc cgtcgctgca
     2701 tatggatcag ggttggcaat atcgaattgc aggctatagg caaagttaaa gccccatgga
     2761 gtagcacaaa atatgccgca caaaggaaac ggtctgaata acgcagtgat gaagaacttc
     2821 ttcagcacac tgctaaaacg catagtgatc gagcgctgat tctggcgaac aactgaacta
     2881 atacatcaga atctgcatta tgttaaataa atataaaaag atggtttaaa taccccgtta
     2941 cttttgactt acactatacg gtatcgcatc gtttaatatt cgcaccggcc agatttttat
     3001 ttctattagt tgtcacaata ctgaatgcgt acgaccacag tattctggct cctgtgtggt
     3061 tatgctttaa ttctgcgttc cgggcagata agcagttgct tgcaggaatc cttcttgtgt
     3121 taatgtcagt tcccctttta ccagtgctga tttccacatt ccgtccaaca gagcttatag
     3181 cctttccctg gattatagca ttgtccggct gaagttcttt ttgaataata atagaagcac
     3241 tgctggcaga tccagtccgt ttttcataac ccactgtact gataaccata atctaatcag
     3301 tagaaattga gtcgaaaata agcactactc catacaggat aattagaggt cagtttgatt
     3361 attcacaatt catcatcagc attttctatt tctgacgaaa tcaatatgaa aataaccata
     3421 tatgataatt attataataa cggctttaat tggaatacat atattacaac gtattatata
     3481 taattggtat tctgggaact atattctcaa aatacagtag aaacgaggta tgtttctggt
     3541 ggaaaggaca gtgggattaa aaagtaagaa ttgataaaaa aacgccagca acacatagtg
     3601 ccggcggagg gaatacccca tggagaaaat gt
//
LOCUS       DAZFTN010000244         3612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFTN010000244 DAZFTN010000000
VERSION     DAZFTN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(120..1277)
                     /gene="intS"
                     /locus_tag="ACHKV2_005099"
     CDS             complement(120..1277)
                     /gene="intS"
                     /locus_tag="ACHKV2_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO6942792.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1439..1513)
                     /locus_tag="ACHKV2_005100"
     tRNA            complement(1439..1513)
                     /locus_tag="ACHKV2_005100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Arg,seq:cct)
     gene            complement(1589..2521)
                     /gene="yfdC"
                     /locus_tag="ACHKV2_005101"
     CDS             complement(1589..2521)
                     /gene="yfdC"
                     /locus_tag="ACHKV2_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO6942793.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2815..3570
                     /gene="mlaA"
                     /locus_tag="ACHKV2_005102"
     CDS             2815..3570
                     /gene="mlaA"
                     /locus_tag="ACHKV2_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO6942794.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          856 a          882 c          857 g         1017 t
ORIGIN      
        1 aacatataat aattgcattt tgttattgtt gatggtttct tggtgtaaga aatcctgaga
       61 agtgaaaagc tttgctatat gtgcaaggct tttctttttt ataagaatcg ttgtaataac
      121 tactccacct tcccatcaag ccagtccgcc caccactgca tcatttctct gcgcttatcg
      181 agatactgag catggttgta aatcccacgc acagatccgc cgttggcatg tgctagttgc
      241 acttcaatag cgtcagcagg ccattcgtgc tcgttcataa tcgtgctgaa ttcatgcctg
      301 aatccgtgac cgctttccag accttcatag ccgatttgtt tgatcacaag cagtacagcg
      361 ttctcacaga ttgacttctt cttatcgttg cgcccggcaa aaacaaactc tgatactggt
      421 ttagtgatgg agcttagcgt agtgagaagt tcaacaacct ggtccgacat cggaaccaca
      481 tgaatcttgc gtcccttcat cacactggcc tcgatggtga taatcctgtt ttcaaaatcg
      541 acgttcttcc attgcatgga acgaagctct ttcgttctta gtgctgtgta gcgtaaaact
      601 ttggtcgcaa tgagagatac gatacttcct gaaaatgttg caagtgcttt gttgaatgcc
      661 gggatctggt cggcaggtaa aaacgggaag ttcttcttgc ggtatccctt catggcttca
      721 gcaaggtcag gtgccgggtt atatttagcc cttccggtga caatagcgta acggaaaacc
      781 tcgccgcatc ttctgcgtgc tttgttggct cgctccattg ccccgcgatc ttcaaatctg
      841 cggattactt ccagcagttg catcggctca atatcctgaa tttcaaggcc gccgatgata
      901 ggtaaaatgt cgtcatcaaa catttttgca agttcagtcg cataggcggc tgaccagact
      961 tgcctcttat gctcgtacca ttccttgtaa atcgcactaa aggaattgtt gttagacgaa
     1021 gcctttttcg cctttaccgg atcgatgcca accgagatgt ctttcctcgc agtccatgct
     1081 ttatcccttg cctcttgcaa agtcataagc ggatattttc cgacggtcag gattttctcc
     1141 ttaccgtcaa tcttgtagcg aagctgccat acctttttcc ctgatacagg gacataaagg
     1201 tacaggccat taccatcgag taggcggtat ggtttttctt tcggctttgc tgcttcaatc
     1261 tgcttaacgg tgagcatggg taaaaatccg gtgggtaaaa ttattttatc cactttttac
     1321 ccgtcatggc gtgcggctgt caacgatctg acgcgaacca tgacgaactg tgaatctacg
     1381 gaaggcttga tattcagggg attttgcgga ctggtacgga tggaggcgaa ctgataaatg
     1441 gtgtcccctg caggaatcga acctgcaatt agcccttagg aggggctcgt tatatccatt
     1501 taactaagag gacaatgcgg catgagtata cccgctaatg gactgcgggg taagtacgct
     1561 gccgctcgat tgcttaaacc ctcgccattt atgccgggtt tttatcattt ttcttaatgt
     1621 tttccgcacg ttctgctttt tggcgtgctt ctgctttgcg cttgttgctc atgtcgttac
     1681 ggatctgtgc atgactcatt aacgcgaaga taaaggtgcc gccgcagatg ttccccgcta
     1741 aagtaggtag tgcgaagggc cagatgaaat cgctccagtg cagcgtaccg ttaaacacca
     1801 gatagaggat ttcaacagaa ccgacaacga tgtgagtggt gtcacccagg gcaataagcc
     1861 aggtcatcaa tataatcacc acaatctttg ccgcacccgc tgcaggaaac atccatacca
     1921 tagtggcgat cagccagccg gaaatgatcg cgttggcaaa catctcgctg ggggtgttct
     1981 tcatcacatc catgccgatt ttgacaaatg catcgcgagt ttcttcattg aagattggca
     2041 tatattcaaa tgcccacgcc gcaatacctg tcccgagaat attacccagc agcacgacgc
     2101 cccataaccg cataagtaag ccaacgttgc tcattgtcgg tttttgcatg acgggtagta
     2161 ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc cataatgacg ataataaaac
     2221 caaaggtata accgagattc tccagcaaga agctgcccgg cacaccttcc agttcgacat
     2281 gaaatatccc ttttgccagt aatgaagcgc ccatcgacag acccgccgca atggctgacc
     2341 acagtagtgc cattgcgtcg cgttccagct ctttttcacc atcctggcgg atatgctcat
     2401 gaattgccat cgcccgggag gggagtcgat cttcatctat ttctattttt ttgccacgct
     2461 ctttttcttc gctctcaact tcaatttcgt cgctgtgttg atcaattttg tcgttgtcca
     2521 tggtctcttc tcttgaatta gcacgtatag ctaaagcgta gcggcttttt tgctcgcaac
     2581 tggcgggagt tactttgaaa atgtagaaaa ggccgcgttt gcctttttct gtttctatag
     2641 aatcaagtag cctacagggc ggcgattacc aggctatgat caaatcagca aatcagggcg
     2701 tctggacatc agttgacgtg ctgttacaat cgcccacacc taaacaggcg gatacggtat
     2761 cgttccgtca tggatggcaa actgcataag ccataaaaaa acagggagac atttatgaag
     2821 cttcgcctgt cggcgcttgc tctgggaact acgcttctgg tggggtgtgc gagttccggt
     2881 acagatcagc aagggcgttc tgacccgtta gaagggttca accgcaccat gtacaacttt
     2941 aacttcaatg tattagaccc gtatattgtt cgaccggtcg ctgtcgcctg gcgtgattat
     3001 gttccgcaac cggcgcgtaa cggtttgagc aattttaccg gcaaccttga agaacctgcg
     3061 gtgatggtta actacttctt gcagggcgac ccttatcagg ggatggtcca ctttacccgc
     3121 tttttcctga acaccatttt ggggatgggc ggctttattg atgttgcagg gatggcgaac
     3181 ccgaaactgc aacggactga acctcaccgc ttcggtagta cgcttggtca ttatggcgtg
     3241 ggttatggcc cgtacgttca attaccgttc tacggtagct tcacgctgcg tgatgacggt
     3301 ggtgatatgg cggatggtct ttacccggtt ctttcctggc tgacctggcc gatgtctgtg
     3361 ggtaaatgga cgcttgaagg tatcgaaacc cgcgcacagc tgctggattc cgacggtctg
     3421 ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag cgtacttcca gcgtcatgat
     3481 ttcatcgcta atggcggcga actcaaaccg caggaaaatc cgaacgcgca agcgattcag
     3541 gatgatttaa aagatatcga ttctgaataa gaaacaaata aaaaaggtga gtcgcaaaac
     3601 tcaccttttt tg
//
LOCUS       DAZFTN010000245         3608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFTN010000245 DAZFTN010000000
VERSION     DAZFTN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..390)
                     /locus_tag="ACHKV2_005103"
     CDS             complement(<1..390)
                     /locus_tag="ACHKV2_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO6942795.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVI"
     gene            complement(390..575)
                     /locus_tag="ACHKV2_005104"
     CDS             complement(390..575)
                     /locus_tag="ACHKV2_005104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942796.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            752..2962
                     /gene="katP"
                     /locus_tag="ACHKV2_005105"
     CDS             752..2962
                     /gene="katP"
                     /locus_tag="ACHKV2_005105"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO6942797.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3006..3395
                     /locus_tag="ACHKV2_005106"
     CDS             3006..3395
                     /locus_tag="ACHKV2_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6942798.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3452..3608
                     /locus_tag="ACHKV2_005107"
                     /pseudo
     CDS             3452..3608
                     /locus_tag="ACHKV2_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3508..>3608
                     /locus_tag="ACHKV2_005107"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1028 a          760 c          922 g          898 t
ORIGIN      
        1 tatcacatcc gcagcaatgc ggctcatctc tgccagtaac caccggttgt ggaacaccag
       61 ggaccagtac tggcagggaa gtgtgaacac aatatgctgc cacggacagt cgggaaccag
      121 actcagcaga tactgtatcc actgtgcgcc agccttcact ccgcagtgcg ggcaggagcg
      181 acttttacac cggaagcaga ccttttttat gtggctgcag tccggagatg aacagcacca
      241 ctgtgtgtac cccatcagcg tggtcccgca ggccatgatt tttgtcacag actcaatgac
      301 taccggacgc actgaacctt ccggttgttt ctccagccag ttaagccagc ggtttccctg
      361 ctgaaaaatg tcggcaaaac ggggaagcat cagaagggcg ggggaactcc gtccggccag
      421 tgaaccgtgc cacactccgg gcagtacatg ccgccggcgc tgataccggc aagaatggtc
      481 gcaaactccc gctccgtgca gcgggctatt tcaggatacc cttcgtcatc aacacgtaca
      541 aaccagaaga ccagcttttt gtttctgaca tccacaaaga agggaatatt caggtctgcg
      601 cagcactcaa cggcatcgtc agttgcggct tggaacccct tagtattttt tgtctgtagt
      661 atctatccca gcaataggta tatcctgttg catcaataaa gttgactttt gtatacaaca
      721 tgcgaatttc ccttaatccg gagctattcg tatgataaaa aaaactcttc ctgttctgat
      781 tcttctggcg ctatcgggga gcttttctac cgctgtagcc gctgataaaa aagagactca
      841 aaatttctac tatccagaaa cactggattt aactcctctg agattacaca gccctgaatc
      901 aaatccctgg ggggctgatt ttgattatgc caccagattt caacagctgg atatggaggc
      961 tctgaaaaaa gatatcaaag atttgctgac aacttcccag gattggtggc ctgcggatta
     1021 tggtcattat ggtcctttct ttattcgtat ggcttggcac ggtgccggaa catacaggac
     1081 atatgatggc cggggaggcg ccagtggtgg tcagcaacgt tttgaaccgc tgaacagctg
     1141 gccggataac gttaatctgg ataaagcccg tcgattgctg tggccagtca agaaaaaata
     1201 cggctccagt atttcctggg gagacctgat ggtcctgact ggtaatgttg cccttgaatc
     1261 catgggattt aaaacgctgg gatttgctgg cggaagagaa gatgactggg agtcggacct
     1321 ggtatactgg gggcctgaca acaagcctct tgcagataac cgggataaaa acgggaaact
     1381 tcagaaacct cttgccgcca cgcagatggg acttatttat gtcaatcctg aaggccccgg
     1441 tggaaaacca gatcctctgg cttccgcgaa agatatcagg gaagcttttt cacgtatggc
     1501 catggatgat gaggagactg tggccctgat cgcgggaggg catacatttg gtaaagcaca
     1561 tggtgcagcg tctcctgaaa aatgtattgg cgcagggcct gatggtgcac ctgtggagga
     1621 gcagggactg ggatggaaaa ataaatgtgg tacaggaaac ggcaaatata ccatcaccag
     1681 tggcctggaa ggagcctggt cgacatcgcc aacccagttc acaatgcagt atctgaagaa
     1741 tttatataaa tatgaatggg agctgcacaa gagtcctgcc ggtgcttatc agtggaagcc
     1801 taaaaaagcg gcaaatatag ttcaggacgc gcatgatccg tctgtcctgc atccgttgat
     1861 gatgtttacg acggatattg ctcttaaagt tgatcctgaa tataagaaaa taaccacccg
     1921 tttcctgaat gatccaaaag cttttgagca ggcattcgca agagcatggt ttaaactgac
     1981 ccaccgggat atgggaccgg cagcccgata tcttggtaat gaagttcctg cagaatcatt
     2041 tatctggcag gatcctcttc ctgcggcgga ttatacaatg attgatggta aagacattaa
     2101 gtcgctgaaa gagcaggtta tggatttggg tatccctgca tctgagctga taaagacagc
     2161 ctgggcttca gcttccacat ttcgtgtgac tgattatcgt gggggaaata atggtgcccg
     2221 catcaggtta cagcccgaaa ttaactggga agttaatgag cctgaaaaac tgaagaaagt
     2281 actggcatcc ctgacctcat tacagcgtga atttaacaaa aaacagtctg acggaaagaa
     2341 agtgtcgttg gctgatttaa ttgttctttc gggtaatgct gcaatcgaag atgcggccag
     2401 aaaagccggg gtggaacttg agattccctt tactccggga agaactgacg cctctcagga
     2461 gcagacggat gttgcctcat tcagtgtact ggagccgaca gcagatggat tcagaaatta
     2521 ttactcaaaa agcagaagtc atatatcgcc ggttgaaagc ctcattgata aagccagtca
     2581 gctggatctc accgttcctg aaatgacggc attactgggt ggtctgcggg taatggatat
     2641 taatacaaat aattcttcgt tgggagtgtt taccgatacc cctggtgttc tggataacaa
     2701 gttttttgtt aatctgctgg atatgtcaac acgatggagt aaagcagata aagaagatac
     2761 atacaatgga ttcgatcgta aaacgggagc attaaaatgg aaagcatcct ctgttgattt
     2821 aatcttcagt tcaaatcctg aattacgtgc ggtggcagaa gtatatgcct cggatgatgc
     2881 gagaaataag tttattcatg attttgttaa atcgtggaat aaagttatga atagcgatcg
     2941 gtttgattta aacaataaat gataaaactg atgagggagg gcacctccct catgaaggag
     3001 aaattatgca gaaaagcaat aaagttataa tcagctgtgg tatggcatta tattttttgt
     3061 gtggggggat tacagtggca gaagaacttg atgaaagcat gcaagttctt gcaaggaata
     3121 tgagtgtact tcagtcatca atggataaaa aggagcttct tgaagctctt gacagaatgg
     3181 atgatgctgt agatgagtca atgaagcgtc ttcctgagag catttcaccg gctgatgaag
     3241 aagggaagaa tgactatata aaagaacttc aaaagcttga aaaagagatt atgctggcga
     3301 aacaaaaagt cttatcggga cagttatcag atatacccga ttcggtaaac aagatgaata
     3361 aaatcagagt tgaggggcat gataaattca gataaatcag ttggttgatt cctgcgggac
     3421 gaatacatac gtcaggaaca taactttatg gctggaacgt gaaaatcgtg aactgcgccg
     3481 cagtaacgat atccttcgcc aggcttccgc ttattttgcg aaggcggagt tcgaccgcct
     3541 ctggaaaaaa taatgccact gctggataag ctgcgtgagc agtacggggt cggaccggta
     3601 tgcagtga
//
LOCUS       DAZFTN010000246         3593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFTN010000246 DAZFTN010000000
VERSION     DAZFTN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..472
                     /locus_tag="ACHKV2_005108"
     CDS             <1..472
                     /locus_tag="ACHKV2_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO6942799.1"
                     /translation="SEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRGA
                     LGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNFKLA
                     LSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNILKTI"
     gene            504..1796
                     /gene="sctN"
                     /locus_tag="ACHKV2_005109"
     CDS             504..1796
                     /gene="sctN"
                     /locus_tag="ACHKV2_005109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO6942800.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1786..2244
                     /locus_tag="ACHKV2_005110"
     CDS             1786..2244
                     /locus_tag="ACHKV2_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO6942801.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            2222..2455
                     /locus_tag="ACHKV2_005111"
     CDS             2222..2455
                     /locus_tag="ACHKV2_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942802.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            2528..3097
                     /locus_tag="ACHKV2_005112"
     CDS             2528..3097
                     /locus_tag="ACHKV2_005112"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO6942803.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            3057..>3593
                     /locus_tag="ACHKV2_005113"
     CDS             3057..>3593
                     /locus_tag="ACHKV2_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFO6942804.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIGFSKTCYRT"
BASE COUNT         1205 a          648 c          811 g          929 t
ORIGIN      
        1 ttcagaggta attcagcgtc tgatccagga gcgaatttca gtgcgcaata tgcggttagt
       61 tatggaggcc ttagcattat ggagcccacg agagaaagat ataatcacac tcgtcgagca
      121 tgtacgaggt gcattaggtc gctacatttg ccataagttt tcatattctg gagaaattaa
      181 agcaattgtg atttcgcctg aaatagagga cagaatcaga gatggtgtca ggcctaccgc
      241 tggcggtact tttcttaatc tcgatgcatc cgaagctgag atgattctgg ataattttaa
      301 actggcactt tctggaatca atattcctat taaagacatt attttactgg gatctgtgga
      361 tattcgtcgt tttatcaaaa aacttattga atctagttat cgagacctcg aagtactttc
      421 gtacggggaa ctaacggaaa atgttcccgt taatattctg aaaactattt aggataatgt
      481 cttgcgatat ggagcatgct ggtatgaaaa aattaaaatt attaaataaa tacagctact
      541 tacatagcat aaacggctct ctgattgaag cagaacttga cgacgtttct gtgggtgaag
      601 tatgcgaaat ttatgcgagc aggcaagcaa atgaacgtat agccagggct caggtcgttg
      661 gctttcgcaa tgggaagaca ttattaaacc ttataggcag ctctgtgggg cttacacgta
      721 ctgcagtttt gaaaccaacg ggagagcagt taaccataca gatcagtgat gcctttcttg
      781 gttcggtttt gaatgcctct ggtcaaatta tggaaaggtt tgtcccggac actccaggcg
      841 ataggggaaa tttacgcctg attgatgaac tgccaccgtc gtatcaggaa cgtcgagtta
      901 ttaatacacc acttgagaca cggataagag tcattgatgg cgtactgaca tgtggcattg
      961 gtcaacgtgt tgggattttc gcctcggctg gctgtggcaa aacggtttta atgcatatgc
     1021 tcgtaaacaa tactgaagct gatgtgtttg ttattggctt gattggcgaa cgtggtaggg
     1081 aagttacgga atgcgcggaa tcgctcaaaa aatcagtaaa tgcagcgaaa tgcgtattgg
     1141 tctatgccac ctctgatttt tcgtcagtag accggtgtaa tgccgccctg atggcgacaa
     1201 ccgtggcaga gtattttcga gatcgaggaa agagagttgt actttttatc gactcaatga
     1261 cgcggtatgc acgagcacta cgtgatatga aactggcagc gggtgaacca cctgccagac
     1321 gtggttatcc tgcttctgtt tttgacagtt tgcctcgctt actggagagg ccgggaccaa
     1381 cactaaaagg aagtattacc gagttttata ccgtgttact ggaaggggaa gatgaatcag
     1441 acccactggg tgacgaaatt cgctcaatcc ttgatggcca tatctatcta agtcgcaaac
     1501 tcgcgggtca aggtcactat ccggcaatag atgttctaaa aagtgtgagt cgagtatttg
     1561 gtcaggtcac tgatgaaaaa cacagagata atgctgcacg ggtaaggaag aacctcacaa
     1621 ctttggaaga tctacaggtg tttattgact taggggaata tcgcgcaggg caaaatgctg
     1681 aaaatgattt tgctatgaat gcccggccta aactcacaaa ttggttgaaa cagtcagtta
     1741 atgaaaaaat gccaatgagt gaaacgctaa aagagcttga acgaattgtt aagtaaagtt
     1801 aatcgactta tcagaagaac agcgcagagc ctggctgcat gtgaagcatc attacaaaag
     1861 ttaaatgcag aaaaagaaaa gttagccgaa aaggaacgcc tgtatgatat gcaacttaaa
     1921 aacctgcagt cactgttaga tatgaaggag ttactcggcg aagttgtatt tcgccaggat
     1981 atattttact cgttacgtaa agttacagtc attcagcagc agatcgctga gataaatctt
     2041 gaaaagcaaa aaatagccga gcggcgaaaa atacttaaca aggaaatagt tcaacagcaa
     2101 gcacaaagaa aacactggtg gctaaaagga gaaaaatacg accgactaaa aaagcgcatc
     2161 aaaaaacaac ttttaaatca gatgctttat caagacgagc ttgaacagga ggaaaaatat
     2221 aatggacgaa gtcaagaaaa ttgaacattg taaaatttta aacacagaca cacccgataa
     2281 ttctgcttcg gatttagaca gtttcttaaa gaaaaacaaa aaaaataaag aaatgagtga
     2341 gattgtcgct ggaaccattc tccagttatt acgtcagact caggataacc aattttctac
     2401 gttggggaaa aagaatgggc acatgaagtc tattgacaac aaatcgtctc tctaggttga
     2461 caggaatcca tctctttctt tgcaaacaaa aggagccgag caattttcta caaagaaact
     2521 tctcaatatg acttcacgag atcagggtat taattctgaa ctatctaatc gaacgataca
     2581 atttaaggaa aaaatccata atggtatcca tacagaatac atcacagatc aaaaacacag
     2641 taataataaa gatcgtgaaa aaaaataccg cgatggagac aaaataaatg gaccgcaggc
     2701 ccattcatta gacattacta acgaaagaag gtttgctgat aacagaacca tgtttactca
     2761 acatatcgaa aagcaaagga atgttaatac gctcaatcag aatgatataa ataattctgc
     2821 taataatgcc aatgtgcgcg aaaatgaatt gacatatcag tttcagcgtt ggggacaaaa
     2881 tcatacggtc cggattctgg aaagtagtga agggatacga ctaaaaccat cagacacact
     2941 tgtctccgac agactccatg aagcccaaca caatgatgtt acagcacaac gttgggtatt
     3001 gactgagcaa gatgaacgac aaggacaacg acatcagcct catgaggagc aagaaaatga
     3061 aggcaaattc gaaaacgatc agaaggatga aagttaatgt tcggtctgag gaaagtaaat
     3121 cgaaacactc tacgtttgaa accacttttc aaaattggaa agaaaatggc gaagatgttg
     3181 cattattaat gcccgaattt agtgctaaat ggctaccgat agcagaagag agtggcagct
     3241 ggtctggatg ggtgttgctt cgagaaatat ttcccttaat ttctgccgag ctcgctggga
     3301 tggctttaat gccagaaacg gaaaggttaa taggggaatg gctcagttta tccagttccc
     3361 cgttaaatct aaagtatcct gaactaaaat ataatcgttt gtgcgtaggt aaagtatttg
     3421 atggagtatt gagtccagct caaccactga taagaatatg gacaggggaa ttgaatcttt
     3481 ggttagataa agtcacagtc tgccaatacg aaaacgctcc aactttagac aaaaaatcat
     3541 tatattggcc cattcatttt gtaattggat ttagtaaaac atgttataga act
//
LOCUS       DAZFTN010000247         3566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFTN010000247 DAZFTN010000000
VERSION     DAZFTN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..182
                     /locus_tag="ACHKV2_005114"
     CDS             <1..182
                     /locus_tag="ACHKV2_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO6942805.1"
                     /translation="AGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEAR
                     RSADEWLCRYDLPKV"
     gene            217..645
                     /locus_tag="ACHKV2_005115"
     CDS             217..645
                     /locus_tag="ACHKV2_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942806.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASVLVPDDVADRVRRAVCEIRACEAGADPR"
     gene            629..847
                     /locus_tag="ACHKV2_005116"
     CDS             629..847
                     /locus_tag="ACHKV2_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942807.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            858..1085
                     /locus_tag="ACHKV2_005117"
     CDS             858..1085
                     /locus_tag="ACHKV2_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6942808.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            1066..1374
                     /locus_tag="ACHKV2_005118"
     CDS             1066..1374
                     /locus_tag="ACHKV2_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO6942809.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            1371..1661
                     /locus_tag="ACHKV2_005119"
     CDS             1371..1661
                     /locus_tag="ACHKV2_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO6942810.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            1664..2245
                     /locus_tag="ACHKV2_005120"
     CDS             1664..2245
                     /locus_tag="ACHKV2_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO6942811.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            2245..>3566
                     /locus_tag="ACHKV2_005121"
     CDS             2245..>3566
                     /locus_tag="ACHKV2_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942812.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKW"
BASE COUNT          768 a          964 c         1087 g          747 t
ORIGIN      
        1 gggccggtga aacgaacttt cgcaactcca cgcttctgcg cctgatcaat gccgggcaga
       61 tgcccgcgtc ctgtaagcag tatatccgct ggatttattc aaagggggtg aaactccccg
      121 gccttgaggc ccgccgttcg gcggatgaat ggttatgtcg ctacgacttg ccgaaagtct
      181 gaaccgcttc tggcgaccgc tcatgatagc gctgctgtgt gcggtactgc tgttacgggg
      241 tgtcctgtgg ctgcggtgaa taatgcctta ccggccctga ttgccggggc gtgcatggcg
      301 gcgctgggga ttatcgctgt actggttgcc gctatatggg gaatgcacca gaagacgcag
      361 cgccttgaag acaataatca ggtgcttgtg cgtgagcggg atgaggcgcg tcaggtgctg
      421 gcaaatcagc agcacaccct gcaactcatt tcacaaatca gtgaggcggc cacgaatgaa
      481 aaacaacaga acattcagca cagcgagggg cagcagagcg ttgtccgccg gtcgctggcg
      541 gcagtgcctg cggcctctgt ccttgttcct gatgatgtgg ctgatcgggt gcgccgggcc
      601 gtctgtgaaa tacgtgcctg tgaagccggt gccgatcccc gctgaatggc tggctgactg
      661 cctggtccct cctgcgccgg agccgttcac gtttggggca tcggtcactt acaacctgca
      721 actgctggcg gtgatcaaga actgcaacgt ggacaaggcc agtattcgtc gtctggaggc
      781 gcggcgacaa catgaattta ctgatatggc cggaacgcct gctgttccgg caggaaagac
      841 gaagtaagga aaaaggtatg gatgattcag atcgtgctca ggctgtgatg gagcggggat
      901 cagaacgcgc cctttgtaac cggctgacac gtaagcgccg ggcaatggat gctccgggca
      961 gacgtgtctg cgcggactgt ggcggagaga tccccgccgc acgtcttgtt gctgtgccgg
     1021 atgccatccg ttgtgtgaac tgccagaaca tcatggaggc ccgtcatgtg ggtcagcatc
     1081 gttaaagatt atgttgtgcc gatcctttcg gcgacggcaa ccgctggcgg gatattcatg
     1141 gcgctgatgc gcaaaacgtt tgtcccccgc gaggcctttg aaaaactctc cgaccgcgtt
     1201 gagaaggtgg aaacccgcct gtcgtcactg ccgacagaag ccgaggtcaa ccgtctgaat
     1261 gtggaaatcg tgaccctgcg gggtgaactg aaaaccacga acgccacgct ccgctctgtc
     1321 tcctatcaga acgaactgct gctggagcag gctgtaagga aaaaaacgca atgagtgatt
     1381 tcattactga agatcagcgt cttgtcattc tgcggtcgct ggcggattac aacggcgaac
     1441 tgggtgaatc cgtgctccag gactgtctgg atgattacgg tcaccgcgtg tcccgcgaca
     1501 ccgttcacac ccacattgcc tggcttgccg agcagggact ggtgcgtaag cgcacgctcg
     1561 ttaacggtta tttcatcgcc gaactgaccg gccggggtca ggatgtggcg gaaggtcgcg
     1621 ccagcgttcc gggtgtgaag aaaccacgcg caagggggta acgatggaca agccgacgcg
     1681 tggacgcgtg cgcaaggtgg atttgctccc cgacagcatc cgtaagccac tgctggaaat
     1741 gctgcgtgaa aaacgtctga cgcaggtcca gatacgcgag gaaatcaacc gtctgatccg
     1801 tgaggcgggc ctgccggaag agcagcagct ttcaccggcg gccatcagcc gggaagcctc
     1861 ccgtaatgag ctgattgccc gtaacctgcg tgatttacgc gagcagacaa aagccatgat
     1921 ggccgaactg ggagacaagc cgaccggtga aaccacagcg ctgattctgg agatgtcccg
     1981 cgccctgatg taccgccgtc tgcgtgccgc gaccgaatcc ctgaacagtg acagcgacgt
     2041 ggatatgcgt cttattaagg acatcctcct gtcagcacag cgtgcggaaa gtgccgccga
     2101 gcgcagcatt aagcgtgaaa aagaaatccg ggccgcattt gctgaagaga tggcaaacgc
     2161 cgtcaccgac gagctgcgcg gcgtggacgg gatgagtgaa cagcttgagt cccgtattaa
     2221 ggggattctg ttgggtaaag cctgatgaat aacgccgaag aactctcccc cttactgacg
     2281 aataccgtct ccacccgcaa aattgatctg gccggtgaaa aagccctgct gggcgtggat
     2341 gtgccggaca gccttgattt gcccggtgat atgccggtat ttctggatta tcaggcccgc
     2401 tggtttgagg atgaaagcga ggtctgcatc gcggaaaaat cccgccgcac cgggcttacc
     2461 tgggcggaag ccgggcgtaa cgtcattacc gccgcaaagc ctaagcggcg tggtggccgc
     2521 aatgtgtttt atgtggggtc aaagcaggag atggcgctgg aatatatttc tgcctgcgca
     2581 ctgttctccc gtgcctttaa ccagctggca gatgctgatg tgtatgagca gaccttctgg
     2641 gatcgggata aaaaagaaga aattctgacc tacatgatcc gctttccgaa cagtggattc
     2701 aaaattcagg cactgtcttc ccgtccgtca aacctgcgcg gccttcaggg ggatgtggtg
     2761 attgatgaag ccgcgttcca cgagtcactg gatgagcttc tcaaggcggc aatggcgctc
     2821 accatgtggg gcgcgcgcgt gcgtattatc tccacgcata acggcgtcga taatctgttt
     2881 aatcagtaca ttcaggaagc ccgcgagggg cgcaaggatt acagcgtcca ccgcattacc
     2941 ctggatgatg ccattgcgga cgggctgtac cgtcgtatct gttatgtcac cggccgcgaa
     3001 tggtcaccgg aaagcgagca gaagtggcgt gatgatctct acaaaaacgc cccgacccgt
     3061 gaggatgccg acgaggaata cggctgtatc ccgaaaaaat ccggcggtgc ctatattcct
     3121 cacgcgctta ttgaaatggc gatgctccgc gacatcccga ttctgacgtt tgaagccccg
     3181 gacgacttca tcagccgtgc cgcatggctg cgtgaatcgg aggttttaac ctggtgtgaa
     3241 gaacatttaa aaccgctttt agaggcgtta aatccccgtt cccgcttcag cttcggggaa
     3301 gactttgccc gcaccggtga cctctcctgc tttgtgctgc tggaaatcac tgaatccctg
     3361 gcaaaacgcg aggtgtttcg cgtggagctg cgtaacctgc catatgccca gcaggagcag
     3421 gtgatgatgt acatcctgac ccgcgttccg gcgctggtcg gtgcggcgtt cgacgccacc
     3481 ggtaacggcg gctatctggc cgaagccgcg ctgctggcct ttggcccgga catcatcgac
     3541 tgcgtgatgt tatcgccgaa gtggta
//
LOCUS       DAZFTN010000248         3503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFTN010000248 DAZFTN010000000
VERSION     DAZFTN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..844)
                     /locus_tag="ACHKV2_005122"
     CDS             complement(<1..844)
                     /locus_tag="ACHKV2_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO6942813.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIIS"
     gene            complement(854..1534)
                     /locus_tag="ACHKV2_005123"
     CDS             complement(854..1534)
                     /locus_tag="ACHKV2_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO6942814.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1521..1988)
                     /locus_tag="ACHKV2_005124"
     CDS             complement(1521..1988)
                     /locus_tag="ACHKV2_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO6942815.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(1988..2524)
                     /locus_tag="ACHKV2_005125"
                     /pseudo
     CDS             complement(1988..2524)
                     /locus_tag="ACHKV2_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2588..3235
                     /locus_tag="ACHKV2_005126"
     CDS             2588..3235
                     /locus_tag="ACHKV2_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6942816.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT          963 a          843 c          822 g          875 t
ORIGIN      
        1 ttgaaataat accaccatta aacgaaaaca ctaatattga aaaggccatt gatggtataa
       61 aaagcgcatc atactgtaat ccattgctat tttgactttt tgccaccata tagacagaca
      121 ttaggataaa gactacagat gatatttcag caaatgatag aactaaatat gacggctttg
      181 acttaaactc aaaatctccg gcctgattgc tgcggattgt ctctgccagc ctgcctcggt
      241 caatctcacg accagccatc ttgtcctgaa cattgaagta gtcaatcgga ccgagagcag
      301 ccatcccaag gtgataaaca aactcaccaa accctgaagg attctgctga tacatctgag
      361 caacgttgtt agggtcaaca ccgacgcgcg ccagttcctt ggcgttgttt tgcagccatg
      421 attgcattgc ttctggagac gaggccgcaa ggcgtgcgcc agccgctaag gtgccgatag
      481 aattgcgctg gtcttcgtct gcccacttca taccagactg aatcttctct aattgaccag
      541 gatatttggt catcagatct cgaacctgct gtcgatcgcc ggactggatg gctgccgcat
      601 attctttttg gaatgcagca tccgcttcct gttgctttgc ggcttgagat gtttgagcga
      661 cactaccaag tccctgcaac gcctgaaggc cgatgttatt gcgacctgaa cgctccattt
      721 cgttgttctg gcgaatatag gccaacgcct cacttacatc acttgccttt ggcgcatttg
      781 agttttgccc accgatacca gcaagaaaac cgcctgagtt gattccttgt tgccaagtag
      841 ccatattccc accttaaaac aatgatccaa gaccaccgat aacaccgcca gcaagagcac
      901 caataccagt cccaataaca ggaactgcac taccaatcat tgcacctgat gcggccccac
      961 caatggcacc tgtagcaaaa ctctgtaggc ctgaaggctt attcgcattt gctgccgatg
     1021 ctgccgcctg ctgttgatac aattggctga cattgttagc gtagttctgt ccggcgtttg
     1081 cctgacctgt aagagcacca aggccgatat ttgccagatt gttgtagttg ttcatctgac
     1141 ctgacagcca gttttgaccg agtgtaggtg cgattgctgc taactggttt cctgttgctg
     1201 tagagcctaa tccacccgtt gcctctgctg ctgccagact ctgatagcgc gcctgccctg
     1261 caaggtcttt atactgctga gagttgtaat actggttaag cgcctgacct tgcccctgaa
     1321 gagaggaaag attctgcaac tgtgatacgt actgctgagc gagtggcgtg aacggtgcaa
     1381 ggttttgcat gttcgtctgc cacatttcac gctgcagttc gatgcccttt tcagttgcgc
     1441 gtgcctgggc tttagatccg gagtcgctgc cgcctttcat atacccattc atgggaagca
     1501 atttattctt gaagctttcg ctaagtacta acatttaata gctcctcata tttcgaacga
     1561 ggtaattgat agagggtgat tcctaccggt tttccgttac tcatgtacgc atcatcaagg
     1621 tgaccaacac gggtagcgcc aagcaaacgg ataattgccc gtccgtattt ggtggtgtca
     1681 ggaaccatgg tgatgctgtt aaggaatggt gagttttcga gaagccattt gcagaataat
     1741 cgatgccctt gtagtgcata ttcgccacgg aatccggggt catacaccgc atggcattca
     1801 acaacgctat gccagaagtt acgcacttca tgaactccag ccagcactaa tccttcgtag
     1861 atgccgagat ataccgcatc aggcttgatg tagtatttat ctccactgtc tacgatattc
     1921 cccgtgttta ccgggttgtt aaggaattct gcaagtttca ccgggttatc gatgagcttt
     1981 atttccatca ctgctccgca atgattttga tggttgtggc agtaaacgcc gcgccattcg
     2041 actgaatggt taacgtgctg ccatttgtgg caagaaagcc gtctttatcc acgctgaaga
     2101 acgtagctaa caggatgttg tcggttgttg ttgccgagtt gcgactgctt actagcgtgt
     2161 caggaacaga accggagaat gttagctgca ttgacctgtt ggcggttccg ctgggccacg
     2221 tcccgacaat cgacagcttg aagaacaagg ttttgttctc gttgaacaca accatcttgt
     2281 tgttaacggt gtcgaagaat ggtgccaacg tcccggatga cggcgtaagc gttttcagca
     2341 ggctaacaag gttggtcggc gctgtcggga tggttactga tacgccagag taaacaacct
     2401 ctgacttctt gcgcgtagtg gcatactcca gagcattaat gcgcgtttca tggtctgaaa
     2461 cctgcgattc cagcgactga actctggtat caagcgatgc aatatcgctt tcattcaagg
     2521 caatcagtgg gtttacgtca tggagtgggc tcaacaccag tcagagccgc atgtacacgc
     2581 aataatcatg gtgaacgaaa gaagcattaa tgggcaagct ggcaaaattg tcagcaaatg
     2641 ggtaacactg gcaaacaatg caatcccatc aaatgttcct ccccagtggg atctagattt
     2701 gaaaggtcag caatattcag agcctacaaa gtgtataggt aaacagattg attacatggc
     2761 aaagcccgaa actaagttaa atgactttaa tagagaggcg agcaagagcc tatatgattg
     2821 gtcagatgcc aacgtttggg gttattcgaa tggctgggcg cggcatgaga taaaaaaaga
     2881 aacactttct atcactggct ttcatgctgt aagacgcata ctaaaagccg ttcaggcatc
     2941 aaggaaagac aactacaaag ctaagcgaca gatacagcgt acattgaaac gacaagacat
     3001 aaatcgatca ccaatcaccc cgttccaagc accgttaatt agcaaacacg attgggattt
     3061 atcagtgata gcggtcacca aagaggcaga gacagagcgc agggaattac tatcctcaca
     3121 gataataaac tcgaacaaag tacaaaccca cccactgtta aggatgttaa gaagagcacg
     3181 agtgagagca tccagagcca aaagagaaag cggaggttat gaccattact actagtagtg
     3241 acagatcgtg acaaggcggt gcaaaaaacg accaataata agagagatat gcctgccaaa
     3301 ccgccaaaag cgcggagcgc ggcaaacgcc gcgagaggcg caagaaggcg aacctatcca
     3361 ttgaaacggt cagaagcgca tcgcgctcga cgaccgaagt ccccactaac accgttaacg
     3421 aaagatcaaa gtcttagaaa aaaatgacgt ttctttgatg gacttccatc cgtaaaaatc
     3481 aaattttgag aaaaagtact tat
//
LOCUS       DAZFTN010000249         3501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFTN010000249 DAZFTN010000000
VERSION     DAZFTN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..81)
                     /locus_tag="ACHKV2_005127"
     CDS             complement(<1..81)
                     /locus_tag="ACHKV2_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942817.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSEL"
     gene            367..573
                     /gene="ybcW"
                     /locus_tag="ACHKV2_005128"
     CDS             367..573
                     /gene="ybcW"
                     /locus_tag="ACHKV2_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO6942818.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(738..932)
                     /gene="ylcI"
                     /locus_tag="ACHKV2_005129"
     CDS             complement(738..932)
                     /gene="ylcI"
                     /locus_tag="ACHKV2_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6942819.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            1321..1866
                     /gene="nohD"
                     /locus_tag="ACHKV2_005130"
     CDS             1321..1866
                     /gene="nohD"
                     /locus_tag="ACHKV2_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO6942820.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1841..>3501
                     /locus_tag="ACHKV2_005131"
     CDS             1841..>3501
                     /locus_tag="ACHKV2_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6942821.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
BASE COUNT          883 a          779 c          937 g          902 t
ORIGIN      
        1 tagttcagaa taaaacaatt cacagtttaa atcttttcgc acttgatcga atatttcttt
       61 aaaaatggca acctgagcca ttggtaaaac cttccatgtg atacgagggc gcgtagtttg
      121 cattatcgtt tttatcgttt caatctggtc tgacctcttt gtgttttgtt gatgatttat
      181 gtcaaatatt aggaatgttt tcaattaata gtattggttg cgtaacaaag tgcggtcctg
      241 ctggcattct ggagggaaat acaaccgaca gatgtatgta aggccaacgt gctcaaatct
      301 tcatacagaa agatttgaag taatatttta accgctagat gaagagcaag cgcatggagc
      361 gacaaaatga ataaagaaca atctgctgat gatccctccg tggatctgat tcgtgtaaaa
      421 aatatgctta atagcaccat ttctatgagt taccctgatg ttgtaattgc atgtatagaa
      481 cataaggtgt ctctggaagc attcagggca attgaggcag cgttggtgaa gcacgataat
      541 aatatgaagg attattccct ggtggttgac tgatcaccat aactgctaat cattcaaact
      601 acttaacctg tgacagagcc aacacgcagt ctgtcactgt caggaaagtg gtaaaactgc
      661 aactcaatta ctgcaatgcc ctcgtaatta agtgaattta caatatcgtc ctgttcggag
      721 ggaagaacgc gggatgttca ttcttcatca cttttaattg atgtatatgc tctcttttct
      781 gacgttagcc tccgacggca ggcttcaatg acccaggctg agaaatttcc ggaccctttt
      841 tgatcaagag cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt
      901 gttgttctgc gggttctgtt cttcgttgac atgaggttgc cccgtattca gtgtcgctga
      961 tttgtattgt ctgaagttgt ttttacgtta agttgatgcg gatcaattaa tacgatacct
     1021 gcgtcataat tgattatttg acgtggtttg atggcgtaga tgcacgttgt gacatgtaga
     1081 tgataattat tatcattttg cgggtccttt ccggcgatcc gacaggttac ggggcggcga
     1141 cctcgcggtt tttcactatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc
     1201 gccgtaacct aatgttttta tttaaaacac cccctgaaaa gaaaggaaac gacaggtgct
     1261 gaaaacgggc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca
     1321 atggaagtca acaaaaagca gctggctgac attttcggcg cgagtatccg taccattcag
     1381 aactggcagg agcagggaat gcccgttctg cggggtggtg gcaagggtaa tgaggtactt
     1441 tatgactctg ccgccgccat aaaatggtat gccgaaaggg atgctgaaat tgagaacgaa
     1501 aagctgcgcc gggaagttga agaactgagg caggacagcg agacagatct ccagccaggg
     1561 actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca ggaactgaag
     1621 aatgccagag actcagctga agtggtggaa accgcattct gtactttcgt gctgtcgcgg
     1681 atcgcaggtg aaattgccag tattctcgac gggatccccc tgtcggtgca gcggcgtttt
     1741 ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa agccatgaac
     1801 aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat cgaacagtca
     1861 ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc
     1921 acagaccgcc gttgaatggg cggatgccag ttactatctc ccaaaagaat ccgcatacca
     1981 ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga tgggcagtga
     2041 ctacatccgc gaggtgaatg tggtgaagtc tgcccgtgtt ggttattcca aaatgctgct
     2101 gggtgtttat gcctacttca tagagcataa gcagcgcaac acccttatct ggttgccgac
     2161 ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgactattc gtgatattcc
     2221 gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac cgggataaca cgctcaccat
     2281 gaagcgtttc accaatgggc gtggcttctg gtgcctgggc ggtaaagcgg caaaaaacta
     2341 ccgtgaaaag tcagtggatg tggcgggtta tgatgaactt gctgcctttg atgaggatat
     2401 tgaacaggaa ggctctccga cgttcctggg cgataagcgt attgaaggct cggtctggcc
     2461 aaagtccatc cgtggctcca cgcccaaagt gagaggcacc tgccagattg agcgtgcagc
     2521 cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg gggaggagca
     2581 gtaccttaaa tttggcgata aagagacgcc gtttggcctc aaatggacgc cggatgatcc
     2641 ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgtcagc aggagctgga
     2701 ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg atggcattct
     2761 ctggttttcg tcatccggtg aagagattga accgcctgac agtgtgacct ttcacatctg
     2821 gacggcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga tgaagacgaa
     2881 aggggatacg ggaaaacgta aaaccttcgt gaacaccacg ctcggtgaga catgggaagc
     2941 gaagatcggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc attattcagc
     3001 gtccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc tggatcgcta
     3061 cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg atcggcagat
     3121 tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg ccatcaataa
     3181 aacctatacc cgccggaatg gtgcagaaat gtcggtatcc cgtatctgct gggatactgg
     3241 cgggattgac ccgactattg tgtatgaacg ctcgaaaaag catgggctgt tccgggtgat
     3301 ccccattaaa ggggcatccg tctacggaaa gccggtggcc agcatgccac gtaagcgaaa
     3361 caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc agatttataa
     3421 ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc acttcccgaa
     3481 taacccggat atttttgatc t
//
LOCUS       DAZFTN010000250         3466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFTN010000250 DAZFTN010000000
VERSION     DAZFTN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..706
                     /locus_tag="ACHKV2_005132"
     CDS             <1..706
                     /locus_tag="ACHKV2_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO6942822.1"
                     /translation="SVWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIA
                     FDDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQV
                     VNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPE
                     KQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIF
                     RGMAKRIARLAEQSTD"
     gene            805..1818
                     /gene="ybjS"
                     /locus_tag="ACHKV2_005133"
     CDS             805..1818
                     /gene="ybjS"
                     /locus_tag="ACHKV2_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6942823.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1815..2645)
                     /gene="amiD"
                     /locus_tag="ACHKV2_005134"
     CDS             complement(1815..2645)
                     /gene="amiD"
                     /locus_tag="ACHKV2_005134"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO6942824.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2642..2965)
                     /gene="ybjQ"
                     /locus_tag="ACHKV2_005135"
     CDS             complement(2642..2965)
                     /gene="ybjQ"
                     /locus_tag="ACHKV2_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO6942825.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3176..>3466)
                     /locus_tag="ACHKV2_005136"
     CDS             complement(3176..>3466)
                     /locus_tag="ACHKV2_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO6942826.1"
                     /translation="MNAGNNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARM
                     IFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          817 a          932 c          873 g          844 t
ORIGIN      
        1 ttcggtgtgg tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat
       61 tcaggggctg aaacacgatc tgctggcgga tgacaccgcg ctacgtgcgc tcatcccaca
      121 acggctgatc gctttcgatg acgcggtacg tcgcaccctg aaagaagaag aaaagctggt
      181 caactccagc gactggggat acgacactca ggcctttgcc cgctggcgac cagagtatgg
      241 ttattttgcc aaacaggcgg gatttaccgt taaaacgtcc gccagccttg cggctttatg
      301 gcaggtggtg aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca
      361 gacacgggcg ttgatggacc gtgcgatcgg tcataaatta gcgaaaggcc gtccggagcg
      421 cgaatatttg caaactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga
      481 aaaacaactt acgttgttat ttggcatgaa agcaccgggg ctgggacgac tgtgttttac
      541 cctggaagat aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg
      601 tatgccgggg ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg
      661 aatggcaaaa cgaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc
      721 gttattcttt catgtttccc attgcatcaa gggggaaatc acggaagaat gcgcacgtca
      781 ttttctcaac aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta
      841 ggtcgaaacg cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc
      901 aacgaggcaa tgggtaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg
      961 accgagctgg tctcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac
     1021 tgctccagct ttacctctcc ctgggggaca caacaggctt tcgatctggc taacgttcgc
     1081 gccactcgcc gcctaggtga atgggctgtc gcctggggtg tacgtaactt tattcatatc
     1141 tcttctccct ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc
     1201 cctcaccgct tcgccaacga gtttgcccgc agcaaagcgg ccagcgaaga agtgatcaat
     1261 atgctttcgc aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc
     1321 ggaccgcacg ataaagtctt tatcccccgt cttgcgcata tgatgcacca ctacggcagc
     1381 attctgttac cgcatggcgg cagtgcgctg gtggatatga cctactatga aaatgccgtg
     1441 cacgccatgt ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac
     1501 aacatcacca acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag
     1561 ttgaatattg actgtcgtat tcgttccgtc ccttacccga tgctggatat gatcgcccgc
     1621 agcatggagc gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc
     1681 tcgaagctta attttgactt tacgctggat attacgcggg cacaggaaga gttaggttat
     1741 cagccggtca tcaccctgga tgaaggtatc gagaaaactg ccgcctggct gcgcgaccac
     1801 ggaaaactgc cgcgctaatc ctgcccgtat ttctccagca acgcttcggc aatcgcctga
     1861 gtttctgcat ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaacgccata
     1921 atcacgcgcc gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc
     1981 aaaagctcca gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa
     2041 aagttcaccc gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag
     2101 ggaaataatg gccccggatc gtctttgcgc tgcggggcga tatccgcatg tgccactacg
     2161 ttttccggct tgatgtgata acgggcaata atatctttcg ccagcggaat aagcgcctga
     2221 atctgtgccg gttcaaacgg ggcaaaatat ttcacccccg ccgatttttg ccagccacgg
     2281 ttttccagct caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta
     2341 atccccgcat gccaggccag ttcttgttcc ggcaccaatt gccagatgcg cggtttaccg
     2401 ttgtatcgcg gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt
     2461 gtcgccagcg agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc
     2521 ggatacgccg cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg
     2581 cctttttcgc ctgcgcaccc tgccagtaac agagcgacag cgatcagcca gaagactctt
     2641 ctcatcgacg cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt
     2701 gcccgaccgt ttcgtagtcg atatcaatac cgactacggc atcggccccc agcgcccgcg
     2761 cctgggagcc aagctcctca aaggcgatct cccgcgcttt acgtagctct ttttcgtagg
     2821 caccggagcg tccgccaacg atatcgcgaa taccagcaaa gaaatcacgg aaaatattcg
     2881 cgcctaaaat cgcttcgcca gtcaccacac cgcaatattc aacgatggtc tggccttcca
     2941 gagttggagt tgtggaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct
     3001 aaagcattat cggactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc
     3061 atctcattgt gtcaaaaaca gctacataag cctgtacaat tatctgtttc gtaaccggat
     3121 attggtttca ttatggagca ccgcttacct gccctattat gttgtgccgg caacattaat
     3181 gagtgggaag agtcaactta tattgtaaat gattcctaaa gtcttcccca tcttgcgcct
     3241 gaggtatata ccgctggatt gctttttggt ccgccgccgc aaaacaagaa agtaataaga
     3301 tctcaatgct tttgtcatta atataagcga agatcattct tgcaaattcg tatataaacg
     3361 cccgatcctg agcgcttaat agtttaatac aatatttatt tttaagagaa taaagtattt
     3421 caagaaaagc atccgcagca accttcacgt tattcccggc gttcat
//
LOCUS       DAZFTN010000251         3412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFTN010000251 DAZFTN010000000
VERSION     DAZFTN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..470
                     /locus_tag="ACHKV2_005137"
     CDS             9..470
                     /locus_tag="ACHKV2_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6942827.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRDRGIERQMTEGELKRLAE
                     RQLTKWAKQVGNGMSIPPIRRQLASPKCPQGPTPIELLKQEYERRKAAGFV"
     gene            504..1220
                     /locus_tag="ACHKV2_005138"
     CDS             504..1220
                     /locus_tag="ACHKV2_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6942828.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1217..1534
                     /locus_tag="ACHKV2_005139"
     CDS             1217..1534
                     /locus_tag="ACHKV2_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942829.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1531..1833
                     /locus_tag="ACHKV2_005140"
     CDS             1531..1833
                     /locus_tag="ACHKV2_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6942830.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1823..2140
                     /locus_tag="ACHKV2_005141"
     CDS             1823..2140
                     /locus_tag="ACHKV2_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO6942831.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2094..2411
                     /locus_tag="ACHKV2_005142"
     CDS             2094..2411
                     /locus_tag="ACHKV2_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942832.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2398..2835
                     /locus_tag="ACHKV2_005143"
     CDS             2398..2835
                     /locus_tag="ACHKV2_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6942833.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2837..3028
                     /locus_tag="ACHKV2_005144"
     CDS             2837..3028
                     /locus_tag="ACHKV2_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942834.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            3031..>3412
                     /locus_tag="ACHKV2_005145"
     CDS             3031..>3412
                     /locus_tag="ACHKV2_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942835.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          936 a          718 c         1023 g          735 t
ORIGIN      
        1 ttggcgcgat gactgaacag caaatccggc aggtctgccg tcagtgcatg gaccgctgcc
       61 gggcgggtga aacgtggcct ccggacctgg ctgagtttgt ggcgttgatt tcggagagcg
      121 gggcaaatcc atttggcctg acggtggatg ccgtgatgga ggagtaccgt cgctggagga
      181 atgaatcctg gcggtacgac gggagtgata aatacccgtg gccacagccg gtgctgtacc
      241 acatttgcct cgagatgcgt gacagaggga ttgagcggca gatgacggaa ggtgagttaa
      301 aacgacttgc agaacggcaa ctgacgaaat gggcaaagca agttggtaac gggatgagta
      361 ttcccccgat ccggagacaa ctcgcatcgc cgaagtgccc gcaagggcca acgccaattg
      421 aattactgaa acaggaatac gagcgccgga aggcggctgg ttttgtttga atctgagaaa
      481 cgattttgtc ggaggaaatt ttaatggaaa ccgtatctga cgcactgaaa gcactgaaaa
      541 gagcctcttc acatgtggtg gcagctcgcc ttggaattag tcgtgaagag gctgtcaacg
      601 aactgtggaa actgaaacgc cgtggagaag cggataacaa gggggcgata tggtggctga
      661 ctcaggctgg tgaaagtgaa ccggtgtcac cggtaccgaa agtgacggcg caaatgctga
      721 cagaggcgat tgaacaacat ggcccacaaa cggcggatga actggcattg atgttcggaa
      781 ttacctcccg ccgggcgaat tcatcgctgg caatggcaat cagcaaaggg tgtctgattc
      841 gcgtgaatca ggatggtaaa tttcgttact gcataccggg cgttgattta ccggcagagc
      901 cgaaagcagc atccgtagct gaaacggagg gtaaagccct tcctcagcca gctggtgttg
      961 cgttaccagt ccaggaaacg gctgcacagg aagaaattaa aacagaagcg gtggaggaca
     1021 ttgtgaagtt gcagcaatcg ttcactgaag cgaaagcaga tgacctgatt ctaccatcgc
     1081 tgcatgtggc taaccgcgag ctgcgccggg cgaaaagtaa tgttcagaag tgggagcgag
     1141 tctgtgctgc gctacgggaa ctgaacaaac acagggatat tctccgggat attaccgcca
     1201 ccagagagca gcagcggtga gtggctggaa gaagtggcgc tgggctgaaa tcctgatact
     1261 ccggcagtgt gcgggaacga tgagagtcga aagcatcggt tatctgattg gccgtagtga
     1321 gtcagccgtc aggacgaaag cgcgggaact gggtatcagc atgatgttac ggggtgatta
     1381 tcaccagtca gccaaatgtt cacagcgtga tattgagctg gcgtggcaac tgcatcagcg
     1441 tggtgtaccc cgacgggaaa ttgccgaaaa gtttgggatg aagttgggcg cagtgaataa
     1501 ctacgtttat ttcgacagga gggttcagga gtgagggtga gggtttatat cgccggtccg
     1561 atgacgggat atgaaaattt taaccgcgag gcatttcaca aaacggaaga ggtgctgaaa
     1621 cgggaagggc ataccgtttt aaacccggca gtacttccgg acgggctgac tcagccacac
     1681 tacatggata tttgcatggc aatgctccgt tgcgtggatg cggtttacat gctgaaaggc
     1741 tggcagcagt cggcaggtgc aggggctgag ctggcactgg aggagaaacc gggccatgcg
     1801 gtgatttttc aggaggtggg cagtgaatat tgacccggcg ataacgattg atatggccct
     1861 gaacgccggc ctggcacttc ttggttattt ctacattatg ttctgcagcg gacgatggct
     1921 gtcactgttg ttcatgaaaa aatggaataa acgccgtaag caggagcaac gccagaaggc
     1981 aatggatgca tttttcgaag ccttcggaat tgacggcatg gaaccagggg atccagctcg
     2041 cgcaatcagc agagggggtg tagtaatcct tgtatatcgg agtgaagaga aaaatgacga
     2101 tcacaaaaca acgtgtagaa gaaatcatat cccgcattga aatgtatgga catggtgcag
     2161 ggtatatcgc agacgaggtt aatgatctgg ctatactggc gctgaattta tcaaatatcg
     2221 cgaacctgaa gcgatacgag cttgatatgg gaggttgcga ctcgtgcggt caggattgtg
     2281 gcgctgatat gactgaagat cctgatggtg attatgtcct gtttgatgac gtggttaagt
     2341 tgtttgaatt tgatacaacc actcaaaagt tagaaatccc ggcaaaggag gcagccagtg
     2401 ggcaagattg actatcaggc actgcgtgag gcagcacaaa actatcaatc gacgctggcg
     2461 tggtatcagg ctaccccgga cagcccaaat gctgaacggg attgtgatgc ggctcttgct
     2521 gcgtttaagc gtcacatccg tcatcgggaa gcggatatta tcgctgattt gctggatgga
     2581 ctggaagaag caaaatcaca actcaacgag cagcgtgagt attacgaagg cgttatctct
     2641 gatgggagca agcgtattgc tgaactggaa gcgcgggaag ttcaattacc gactcgctac
     2701 gaccttcgat atggacaccc gataaatgca gatgagcgac atgtcatgat acctaaagaa
     2761 aatggcagtt ggctttacct gattgaccta gaacacgcat tacgcgtcgc tggcattcgc
     2821 atcaaaggag agtgagatga acggacaaat atcaattgtt cgaccaggag catgtgacga
     2881 tcgcgaaata cgaatgatta ttcgtctggc gatggggaaa acaataactg ctctcattac
     2941 tccagaaaat ctcgcattag cattaacagg aaagtcagac atgccagtag agctaaagct
     3001 gcgaaatgtt gagattaagg tgaaatagct atgaccacta ttaccaaaga tcgactgctg
     3061 acaatccagc attggcgcga aacatacgga ccgggtagca acgttgtgct tccagcagaa
     3121 gaagcggaag agctggcacg gattgcgctg gcatcactgg cagcagtatc ggatgaacga
     3181 gcagcctatg aattatttat ggagaagcgt ttcggagaat ctgtagatcg ccgcagagca
     3241 aaaaatggcg atagagatta catggtatgg gatatggcgc ttggctggat tatctggtgt
     3301 caccgcgccg ccatgcttca ggctggaaac tttcgggaaa ataagggttc gtcaaccaat
     3361 aattttcggg aaatctcgga aacgtcaacc aactatccgg taactccgga tg
//
LOCUS       DAZFTN010000252         3395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFTN010000252 DAZFTN010000000
VERSION     DAZFTN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..767)
                     /gene="sctW"
                     /locus_tag="ACHKV2_005146"
     CDS             complement(<1..767)
                     /gene="sctW"
                     /locus_tag="ACHKV2_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HFO6942836.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQS"
     gene            complement(772..2475)
                     /gene="sctC"
                     /locus_tag="ACHKV2_005147"
     CDS             complement(772..2475)
                     /gene="sctC"
                     /locus_tag="ACHKV2_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO6942837.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARMPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2472..3221)
                     /locus_tag="ACHKV2_005148"
     CDS             complement(2472..3221)
                     /locus_tag="ACHKV2_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6942838.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          967 a          735 c          559 g         1134 t
ORIGIN      
        1 gactgaagag acaatagttg tgacaactta ttgagaaaca taccaaattc caatgatgaa
       61 caactcgcat caagtgcatt tatatctgag aaaagcgtct ctttgataaa ttttgtcacc
      121 aacattctat tttgatagcc ataactacca atccattcga cataagtatc tacagcatcg
      181 tcttctgcca ttaaaaattg ccgataactc gcccttagta attttggatt taatgacagc
      241 gcctggccaa ataactttgc ttttaatgcc gagttaattc cgctattaat gaatttttta
      301 gtagtctctt gattaacttt tctaagtaat gcttcagcgt ttagccggac attttctgat
      361 atttgttttt tacgtaaaat tgcacgtaac acaactacca gatcgctttc atctgggaat
      421 aattttttag caaagctata cacttcatag tcatgggtag ttgcagcctt aagcgcctcc
      481 ataactttgt cggttttttc tattggctgg tcttccagga catattcttc tccgtccaga
      541 aaattggcca gttgttcttc gtaaattttt ttcttcataa actgcgttaa cgctgctgac
      601 atctcgtcag tagcttgtaa gaaccgagct tctttacctg cgggatcatc cctcatttta
      661 ggcatttcag cttcaatgtc ttcattatct tctgtgataa tgtcctctaa gcgagaaact
      721 tccctggctc tttctaatac accgacatgc tctacatgaa tcgccatttc actatcctcc
      781 gacgacttca cgattcaggt aagtttttat ccatttctgc agtaattcat cgttgatttc
      841 tttatttttt gccatttgct gtgtaatcgc tctggcatca accgcagcct cattcgcatt
      901 attcatcatg cgttcatcga tctctcttgg ctcaataaga aagacacgaa cgatgttatt
      961 cgcatttgtt ccttcataac caaataattt accaataaat ggaatgctgc ctaatattgg
     1021 gatcttgcga ctttcatagg tattcgtatc gcgtgtatat cccccaatca aaagactttt
     1081 cccttggggc atgcgtgcaa tagtacttat aagtgttcta ccaacctgag gcagatcatc
     1141 aacattggtc ttgtcggaat tgatttcatt tccatcttca atattcaaga gtaattcaat
     1201 ttgatttcgc gcagcaaatc gtggtagcac gctaatcatc gtaccatagg ttacttcatc
     1261 caattccgca gtacgctcgc cgactaattt agtatagaaa gtgcggttat tatcaaatat
     1321 agcaggaata ttctcttgtg ttaaaacgac gggacgagca acaacggcgg ctcttctttt
     1381 ttgtgcaagt gcctgtattg ttgcaataaa ctgtgtccca tcaagggtac taattgaacc
     1441 tgaattatta aatgatgcac ttaagctgga tccaattttt atcgtgcccg accagtcagc
     1501 acctagctgt tctagatcag tcttatcaat atctattatc cataaagata attcaatgtg
     1561 tcgctttgga atatcaagcg tagctactaa cttctcgata aaatcaacct gagatactgt
     1621 tcctttaaca agtaaactgt tggtatcagg atatgcaata atttgtatat cgtcagtctc
     1681 tggcgctcct gctgtcgata ttattttctc tacttgtagg tcatcatctt cttcagcccc
     1741 aggaaaagga ggcatacgtg atacgttttt tagttgttga cgacttgaaa tttcagaaag
     1801 tacgttcacc cctgtgcttt gcttaatgtt attatttaac aacgtcgata gtacttttgc
     1861 catgccagga ataactatct tttcgccacg taactcataa gtccggtcat taacgaatgt
     1921 gttgaccaga tgaattattc ctactttatt tcgaccgatc tcaatgccat cgctgttttg
     1981 ctccattaat ttcgcagcat taacgaccag gtcaacataa accggtggcc ccgatacata
     2041 gaaagtacca ttgccgtccc ctttaatttc ataacgagag ttatacagac ccgatttttt
     2101 taagaaatta ttaaactcat tcgtcgatac ctttctcaga ttaatcaatg cattacgcat
     2161 ctctgatgag tcatagatat aaatagcttt gccatcatca taccaaataa gccccatctg
     2221 tgccgacagg cgttcaatca aggcataggg ttctgttaaa tcaaaatttc cgctgatctg
     2281 tttcttcatg gcaagcttac taactattgt aggcttaccc gcatatgatg aaaccgtttc
     2341 aaaaaaacta cgtagatttt ctttatttgc tacgtatcca ttcgctgctg cggcatatgc
     2401 accaggagtc aataatccat taaaaatgca taagactgaa attaatataa tagtaatgcg
     2461 taattttatt ttcataactt tttacatagt tctctcggtg tttttcctaa ccggcttttt
     2521 acttcggctg aaaagtgtga tgaggacgag taaccatatt tatgcgcaat agttgtcata
     2581 tcactgtttc cttcaatgat ttccaacacg gctctggcaa ctctccagcc acataattcc
     2641 gttttaactt taccacccaa tgcataacta cataaacggc gaaaatgtga atacgatacg
     2701 ccgtaaagct caccaagatc atagagtgaa gtctgtatga gcgattgcga taataaataa
     2761 cgaaccagtt tatagcattc tgtttttctt agatgctgaa acaggatttc attaatatta
     2821 tcgctgctca gataagtggc taaaaaccag gcttcacttg caactttact gaatagcaca
     2881 acttctgatg caaagggcaa ggcatcagca aacaattcat ggtgttccct ttccacactc
     2941 cccgttgatt catctataaa agctaataat ttactcagga tatcaatgcg aagtggatga
     3001 taaattactt tccctgcagt tacctcaatt tggcttcggt caatgagcag taatatatct
     3061 tccactacca ttaatttttg atggttatta ccaatcgtaa taacaagggt tacatccttg
     3121 ttttcatttc cccgtaagaa ccacatttca gggtccagta gggttaactt ctgtccttga
     3181 agcaaaacat tcattggaag taacagccct tcttcaatca tgtttcatct ccttattcca
     3241 tattctcatt ctccataaac ttgctataaa aacatgatta aaaagtgatg attactattt
     3301 ataaaataat gattttgtta tgagcgaata gctatcagcc acttacacta gtacgtcatc
     3361 ctgtccatat acagatttgt aactgttgat atgtt
//
LOCUS       DAZFTN010000253         3364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFTN010000253 DAZFTN010000000
VERSION     DAZFTN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1542
                     /locus_tag="ACHKV2_005149"
     CDS             <1..1542
                     /locus_tag="ACHKV2_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO6942839.1"
                     /translation="EMQSAVLDIPQIASRYIEHPASGITPNRAAQCLRGAERGDLIAQ
                     SDLAADIEEKDTHLFAELGKRRLAIQGVSWSIEPPPNASANEKKDAEMLDEYLHSADW
                     FDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGHFCLNPDDLSELRLRDG
                     SHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNYSVRDLAEFLEVYGLPM
                     KVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQAAANGQADPFETMISWG
                     ERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLRQLAATLNRDLVYPLYA
                     LNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIPDPWVREQTGIPQPAPG
                     EAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKSSPRDELDDMGDAVPAR
                     RLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLMTLLSDAMFAAEMKGML
                     DGTGD"
     gene            1526..2857
                     /locus_tag="ACHKV2_005150"
     CDS             1526..2857
                     /locus_tag="ACHKV2_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO6942840.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            2979..>3364
                     /locus_tag="ACHKV2_005151"
     CDS             2979..>3364
                     /locus_tag="ACHKV2_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6942841.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGAR"
BASE COUNT          763 a          890 c         1042 g          669 t
ORIGIN      
        1 gagatgcaga gtgccgtgct ggatattccg cagattgcca gccgttatat tgaacatccg
       61 gcctcgggta tcaccccgaa ccgggcggca cagtgccttc gcggagctga acgtggcgat
      121 ctgattgccc agtccgatct ggccgctgac attgaagaaa aggataccca cctttttgca
      181 gagctgggca agcgacgcct tgccattcag ggggtgtcct ggagtattga gccgcccccg
      241 aacgccagcg cgaatgagaa aaaggacgcg gaaatgctcg acgaatatct gcattccgcc
      301 gactggtttg atgccatgct gtttgatgcc accgatgcca tcctgaaggg ctattcctgt
      361 atggagattg agcacgggat gctcggtaaa atgcacatca tccgcgccat ccgctggcgt
      421 gacagcgggc atttctgcct taacccggat gatttgagcg aactgcggct gcgtgacggc
      481 agccatgccg gggtggcgtt tcagcccttt ggctggatag tgcatcagtc gcgttcacgc
      541 actggctacg gtggtgcgac ggggcttgtc agaacgctta tctggccgtt cattttcaaa
      601 aactattccg tgcgcgatct ggctgaattt ctggaggtgt acggcctgcc gatgaaggtc
      661 ggtaaatacc cgtccggggc aacaccggag cagaaaagcg ccctgatgcg ggcggtgatg
      721 gatatcgggc gacgtacagg cgggatcatc ccggccggga tgtcgctgga gtttcaggcg
      781 gcagcgaacg gtcaggccga tccgtttgaa accatgattt cgtgggggga gcgttccatc
      841 tccaaagcta tcctcggcgg cacgctgacc acggaagccg gagacaaggg cgcacgttcg
      901 ctgggtgaag tgcataacga ggtgcgccgg gaaatcaggg attctgattt acgtcagctg
      961 gccgccacgc tgaaccgcga tctggtgtat ccgctgtatg ccctgaacac cgcccacgcc
     1021 attgatatcc gtcgcctgcc gcgtatctgt ttccagacaa aagagccggg ggatatcacc
     1081 aaaatcacca gtgcggtgat gcagctcagt acggggatgg atattcctga tccctgggtg
     1141 cgggagcaga ccggtattcc acagcctgcc cccggtgaag ccatcttccg tgtccgtcag
     1201 agtggcaatg aacctgctca gacagacaaa gagatgccac cggaaaaaca ggagaagaca
     1261 gagcagacag cgctggctgc cagactgccg gaagcgaaga gcagcccccg agatgaactg
     1321 gacgacatgg gggatgcggt gcctgcccgc cggttacagg aggccatcga cccggtactg
     1381 gagccggtca ttgaagccat cagaacgcgg gggctggcgg aggcactggc agacctgcct
     1441 gccctttacc gtgaaatgga tgattcccgc ctgatgacgc tgctcagtga tgccatgttt
     1501 gctgcggaaa tgaaggggat gctggatggc acaggggatt gatttaggtt atgccgccac
     1561 gcttccctca aaagaggcgg tggcatactt ccgcgccaaa ggggcgcata tcagctggaa
     1621 ctggttcgaa acagacgcgg atgttcatgc ccgttcattc acggcggcaa aagtggcacg
     1681 cctggacgta ctgaccacac tacaggcgga agtgcagcgt gctgttgatg aggggatttc
     1741 acagaaagca tttatccgca cactgacacc ccgcctgcaa aagctgggat ggtggggaaa
     1801 gcagattgtg gtggacagcg ccggtaacgc agaagaagtg caactgggca gtccccgccg
     1861 tctggcgctg atttacaacg tgaacacccg cgtggcttac aatgccgggc gttacacgca
     1921 gatgatgaac aacacggaca cgcatccgtt ctggcagtat gtggcggtga tggacagccg
     1981 cacccgcccg gcgcattccg cccttaacgg gcaggtattc cgctatgacg atccgttctg
     2041 gaatacacac tacccaccca atggctggaa ctgccgctgc cgtgtccggc cattgtctca
     2101 gacccgtctg gatgcaatgg ggttatccgt ttcatccggt caggatcatc tttccacccg
     2161 caatgttgag gctggcgtgg ataagcagac cggagaagtc agagaaatgc cggtgaccac
     2221 atattcagat ggcaccagaa ccatgacacc ggatgtgggc tggtcatata accccggctc
     2281 ggcggcgttc ggcacagacc aggcgttgat ccgtaaactg atcgaggtga aaagcccggc
     2341 gctgcgggaa atggtagtgc aggagatgaa caacagcccg gagcggcagc tggcgttccg
     2401 catctgggca aaaaacatca tgaaaacccg gcgaggcggt aacgatatcc gcacgctggg
     2461 ctttatgacc gaaagcattg cgcaggcggt ggaaagccgg acgggaacgc caccggcccg
     2521 cctgctggcg atgagcggta aaaatgtgct ccatgcggac agcatgaaac atcagaatga
     2581 cggtatcgcc ctgacgccgg aggatttcgg tcgattaccg gcaatgctgg caaagccaaa
     2641 agcggtgctg tgggacaaac gccacaacaa tctgatgtat atcgtggaaa gtaaggatag
     2701 cagtgtgcag attgccgtta atgcgccgta cagcctgaag cgtcagccgg ataaactgga
     2761 tgtgattgtg aatgcttacc gggttatcaa tatggataag ctgaaatcgg atatccgggg
     2821 tggaatgctt gaggtactgg aaggagatat tgattaacgg tcagtggcgg gggttgaacc
     2881 cgcatacatg atccacccgg aggcaggcac cgactttacc agttaagcgt acactgaccg
     2941 cctgtttatt ttatattcat cctttatgcg gaggcaatat gtcatccatt gatgccgctg
     3001 tcgttgttga cgttacgcgt ctccagcggg tctttgcccg gcttcagttt gtgggtggag
     3061 ggaaagacct ggcccgcagt gtggcggcga gtcttctgtc atcgtcagaa atggcgtttg
     3121 aacaggaaaa agagccggac ggcgaacgct ggcatgactg gtcagatcct taccgcaagt
     3181 ggcgtacccg taagggatac atgcccggca aaatcctgac gctgaacggc gatctggccc
     3241 gacgcctgac cacggattat ggcgatacct gggcgctgat tggatcaaat gagccttatg
     3301 cggccattca tcagtggggt ggcctgcctg gtatgccacc gggaccggcg gccattggcg
     3361 cacg
//
LOCUS       DAZFTN010000254         3362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFTN010000254 DAZFTN010000000
VERSION     DAZFTN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..390
                     /locus_tag="ACHKV2_005152"
     CDS             31..390
                     /locus_tag="ACHKV2_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6942842.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHKV2_005153"
     CDS             399..929
                     /locus_tag="ACHKV2_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6942843.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHKV2_005154"
     CDS             1171..1368
                     /locus_tag="ACHKV2_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6942844.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ACHKV2_005155"
     CDS             1519..2577
                     /locus_tag="ACHKV2_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6942845.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ACHKV2_005156"
     tRNA            2618..2693
                     /locus_tag="ACHKV2_005156"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ACHKV2_005157"
     tRNA            2774..2850
                     /locus_tag="ACHKV2_005157"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
BASE COUNT          861 a          731 c          954 g          816 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aa
//
LOCUS       DAZFTN010000255         3352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFTN010000255 DAZFTN010000000
VERSION     DAZFTN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1542
                     /locus_tag="ACHKV2_005158"
     CDS             <1..1542
                     /locus_tag="ACHKV2_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO6942846.1"
                     /translation="EMQSAVLDIPQIASRYIEHPASGITPNRAAQCLRGAERGDLIAQ
                     SDLAADIEEKDTHLFAELGKRRLSIQGVPWSIEPPPNASANEKKDAEMLDEYLHSADW
                     FDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGHFCLNPDDLSELRLRDG
                     SHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNYSVRDLAEFLEVYGLPM
                     KVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQAAANGQADPFETMISWG
                     ERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLRQLAATLNRDLVYPLYA
                     LNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIPDPWVRDQTGIPQPAPG
                     EAIFRVRQSGNEPAQTDKEMPPGKQEKTEQTALAARLPEAKSTPRDELDDMGDAVPAR
                     LLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLMTLLSDAMFAAEMKGML
                     DGTGD"
     gene            1526..2851
                     /locus_tag="ACHKV2_005159"
     CDS             1526..2851
                     /locus_tag="ACHKV2_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO6942847.1"
                     /translation="MAQGIDLGYAATLASKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKAARLDVLTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGNPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFAQLPAMLAAPDAVLWDHVHQNLLYITETRDG
                     TAKIAVNAPYSVKRQPDKLDVVINAYRVSRNTLIDAVQGGKMEILEGKL"
     gene            complement(2851..2938)
                     /locus_tag="ACHKV2_005160"
     tRNA            complement(2851..2938)
                     /locus_tag="ACHKV2_005160"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2977..>3352
                     /locus_tag="ACHKV2_005161"
     CDS             2977..>3352
                     /locus_tag="ACHKV2_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6942848.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAA"
BASE COUNT          749 a          898 c         1032 g          673 t
ORIGIN      
        1 gagatgcaga gtgccgtgct ggatattccg cagattgcca gccgttatat tgaacatccg
       61 gcctcgggta tcaccccgaa ccgggcggca cagtgccttc gcggggctga acgtggcgat
      121 ctgattgccc agtccgatct ggccgctgac attgaagaaa aggataccca cctttttgca
      181 gagctgggca agcgacgtct ttccattcag ggcgtgccct ggagtattga gccgccaccg
      241 aacgccagcg cgaatgagaa aaaggacgcg gaaatgctcg acgaatatct gcattccgcc
      301 gactggtttg atgccatgct gtttgatgcc accgatgcca tcctgaaggg ctattcctgc
      361 atggagattg agcacgggat gctcggtaaa atgcacatca tccgcgccat ccgctggcgt
      421 gacagcgggc atttctgcct aaacccggat gatttgagcg aactgcggct gcgtgacggc
      481 agccatgccg gggtggcgtt tcagcccttt ggctggatag tgcatcagtc acgttcacgc
      541 accggctacg gtggtgcgac ggggcttgtc agaacgctta tctggccgtt cattttcaaa
      601 aactattccg tgcgcgatct ggctgaattt ctggaggtgt acggcctgcc gatgaaggtc
      661 ggtaaatacc cgtccggggc aacgccggag cagaaaagcg ccctgatgcg ggcggtgatg
      721 gatatcgggc gacgtacagg cgggatcatc ccggccggga tgtctctgga gtttcaggca
      781 gccgcgaacg gtcaggccga tccgtttgaa accatgattt cgtgggggga gcgttccatc
      841 tccaaagcta tcctcggcgg cacgctgacc acggaagccg gagacaaggg cgcgcgctcg
      901 ctgggtgaag tgcataacga ggtgcgccgg gaaatcaggg attctgattt acgtcagctg
      961 gccgccacgc tgaaccgcga tctggtgtat ccgctgtatg ccctgaacac cgcccacgcc
     1021 atagatatcc gtcgcctgcc gcgtatctgt ttccagacaa aagagccggg ggatatcacc
     1081 aaaatcacca gtgcggtgat gcagctcagt acggggatgg atattcctga tccctgggta
     1141 cgggaccaga ccggtattcc acagcctgcc cccggtgaag ccatcttccg tgtccggcag
     1201 agtggcaatg aacctgctca gacagacaaa gagatgccac cgggaaaaca ggagaagaca
     1261 gagcagacag cgctggctgc cagactgccg gaagcaaaaa gcacgccccg tgatgaactg
     1321 gacgacatgg gggatgcggt gcctgcccgc ctgttacagg aggccataga cccggtgctt
     1381 gagccggtca ttgatgccat cagaacgcgg gggctggcgg aggcactggc agacctgcct
     1441 gccctttacc gtgaaatgga tgattcccgc ctgatgacgc tgctcagtga tgccatgttt
     1501 gctgcggaaa tgaaggggat gctggatggc acaggggatt gatttaggtt atgccgccac
     1561 acttgcctca aaagaggcgg tggcatactt ccgcgccaaa ggggcgcata tcagctggaa
     1621 ctggttcgaa acagacgcgg atgttcatgc ccgttcattc acggcggcaa aagcggcacg
     1681 cctggacgta ctgactacac tacaggcgga agtgcaacgg gctattgatg aggggatttc
     1741 acagaaagca tttatccgca cactgacccc ccgcctgcaa aagctgggat ggtggggaaa
     1801 gcagattgtg gtggacagcg ctggtaacgc ggaagaagtg cagctgggta atccccgccg
     1861 tctggcgctg atttacaacg tgaacacccg cgtggcttac aatgccgggc gttacacgca
     1921 gatgatgaac aacacggaca cgcatccgtt ctggcagtat gtggcggtga tggacagccg
     1981 cacccgcccg gcgcattccg cccttaacgg gctggtattc cgctatgacg atccgttctg
     2041 gaatacacac tacccgccca atggctggaa ctgtcgctgt cgtgtccggc cattgtctca
     2101 ggcccgtctg gatgcgatgg ggctgtctgt ttcatccggt caggatcatc tctccacccg
     2161 caatgttgag gctggcgtgg ataagcagac cggagaagtc agagaaatgc cggtgaccac
     2221 atattcagat ggcaccagaa ccatgacacc ggatgtgggc tggtcatata accccggttc
     2281 ggcggcgttc ggcacagacc aggcgctgat ccgtaaactg atcgaggtga aaagcccggc
     2341 gctgcgggaa atggtggttc aggagatgaa caacagcccg gagcggcaac tggcgttccg
     2401 catctgggca aaaaacatca tgaaaacccg gcgaggcggt aacgatatcc gcacgctggg
     2461 ctttatgacc gaaagtattg cgcaggcggt ggaaagccgg acgggaacgc caccggcccg
     2521 cctgctggcg atgagcggta aaaatgtgct ccatgcggac agcatgaaac atcagaatga
     2581 cggtatcgcc ctgacgccgg aggatttcgc gcagcttccc gccatgctgg ctgcccccga
     2641 tgccgtgctg tgggatcatg tccatcagaa cttgctttac atcactgaaa cccgtgacgg
     2701 aacggcaaag attgccgtca atgcgcctta tagtgtgaaa cgtcagccgg ataagctgga
     2761 tgtggtgatt aatgcctata gagtgagcag gaatactctg attgatgctg ttcagggagg
     2821 taagatggag atcctcgaag ggaaattgta agtcggtggt gggggtcgaa cccacataaa
     2881 catccttcgg gtgaagatat ccctcaggtg ccgctttacc agttaagagt acaccgacct
     2941 gcttttattt taaccaggta gtttcacgga ggcaatatgt catccattga tgccgctgtc
     3001 gttgttgacg ttacgcgtct ccagcgggtc tttgcccggc ttcagtttgt gggtggtgga
     3061 aaagacctgg cccgcagtgt ggcggcgagt cttctgtcat cgtcagaaat ggcgtttgaa
     3121 caggaaaaag agccggacgg cgaacgctgg catgactggt cagatcctta ccgcaagtgg
     3181 cgtacccgta agggatacat gcccggcaaa atcctgacgc tgaacggcga tctggcccga
     3241 cgcctgacca cggattatgg cgacacctgg gcgctgattg gatcaaatga gccttatgcg
     3301 gccattcatc agtggggtgg cctgcccggt atgccgccgg gaccggcggc ca
//
LOCUS       DAZFTN010000256         3350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFTN010000256 DAZFTN010000000
VERSION     DAZFTN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(66..1472)
                     /gene="gndA"
                     /locus_tag="ACHKV2_005162"
     CDS             complement(66..1472)
                     /gene="gndA"
                     /locus_tag="ACHKV2_005162"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO6942849.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1673..2338)
                     /gene="perB"
                     /locus_tag="ACHKV2_005163"
     CDS             complement(1673..2338)
                     /gene="perB"
                     /locus_tag="ACHKV2_005163"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFO6942850.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            complement(2382..3313)
                     /locus_tag="ACHKV2_005164"
                     /pseudo
     CDS             complement(2382..3313)
                     /locus_tag="ACHKV2_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          939 a          774 c          659 g          978 t
ORIGIN      
        1 aattggcaaa aaaaagcccg gtacaatata cgccgggcct caattttatt gttgattaaa
       61 tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatgcg cttataagta
      121 tgcgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcggcgcgg
      181 tagctgtcat aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtacc
      241 gcataagcga cgacatcgcg cagcgcctgc tggtagtcat cggcaatttg cttgaagtaa
      301 ggagccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc
      361 aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc
      421 agatcccagt tgtactcttc agacgccgca cgcagttgag agaacccctg agcgtaagaa
      481 acgattttgc ccagatacag tgcacggcga actttttcga tgaactcagc cttgtcgcca
      541 gctggctgcg cttgtgggcc agagagaact ttagacgcag caacgcgctg atctttcaga
      601 gaagagatgt atcgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
      661 agtgcgctct ggctggtcca tttgcccgta cctttgtttg ccgcttcatc caggatcaca
      721 tcaaccaggt agttaccgtc ttcatctttt ttagtgaaga tgtctttggt aatgtcgatc
      781 aggtagctgc tcagttcacc gttattccac tcggtaaaga tctgcgccag ttcttcgttg
      841 gtgaggttca gaccaccttt aagcagagaa taggcttcag caatcagctg catatcgccg
      901 tattcaatac cgttgtgaac catcttcaca tagtgacctg cgccatcggc accaatatag
      961 gtaacgcatg gctcaccgtc ttcagccact gcggcgattt tggtcaggat cggcgcaact
     1021 agttcatagg cttctttctg cccaccaggc ataatggaag gaccttttag tgcgccctcc
     1081 tcaccaccgg aaacaccggt accgatgaag ttaaagcctt ctgcagaaag ctcacggtta
     1141 cgacgaatgg tgtcctggaa gaaggtatta ccaccatcaa tgatgatgtc acctttatcg
     1201 aggtatggct taagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac
     1261 aagatgcgac gaggcgtttc cagagattca acaaattctt tcaccgtata gtaaggaacc
     1321 agtttcttgc ctggattttc ggcaatcact tcttccgtct tttcacggga acggttgaaa
     1381 atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc
     1441 ataccgacta cgccgatctg ttgctttgac attgtttact cctgtcaggg gttaccgcta
     1501 gatatgcggc ttgaataacg tataaaaatg tatcagagta ttataaattt catgctgtat
     1561 cagtaataac taagtaaagg atagcttaaa aagaagccat aatttagtaa attagcgaaa
     1621 ttatatatat tttaaacatt agtcccattt ggaacgtgtt ttcgcattcc cattaaatag
     1681 atgttggcga tcttttcatt tctcttgctg gatttccgca cacagttata ccggcaggaa
     1741 cacttttagt gacaacagcc cccataccta taatggctcc cgcgccaata ataagtgggc
     1801 gattaggaac accctgctta attactgcac ccgagcctat atatgcattg tcttcaataa
     1861 caacatatcc attacattta gccccaggag caaatgtaac atagtctcct atttgacaat
     1921 catgtgcaac gtatgagtat atgtttgcat gaaaaaacct ccctatatga gtattagtag
     1981 atattgtaac aaagggagaa ataatagcgc cactacctat catagtatga tcataaacaa
     2041 cgctatttgg atgttttata gttattggtt caaccccgtg taataatatt gactcagaca
     2101 ctctctgtcg tatcttatca ttagcaatag caacattaaa atactttttt aaataagggg
     2161 cttttagaaa gcagttggtt gaaagcactt caaaaccatt aactttcttt cctgccaaaa
     2221 catcatccac aaaaaccaga gcatagtcag aaccacattc ttgctttatt tgttgattta
     2281 gaatgggtat tgtttctcta ccataacttc cagcaccaaa aataccatac aaattcattt
     2341 tcccatctcc aaagaaatat cttatcaaaa ccaatgcaag attatgaaag cccgcacgct
     2401 gcaatgcctg acgctaggaa gtttctgtcc atgactgctt ttcgcatctt acatgataat
     2461 cctgccttga atacattatc attgtcagat attaatagcg atgtgccgca ttctagaaaa
     2521 tcattcgaat gcaactcgcc ttcttatatt atagtcgatt tcattcatta ccacatcagg
     2581 ctactgtgca acttattctc catgcgccag tgatttcgat ttactgtggc gaacttctct
     2641 gcggttaaag cagcagatct aatataatat ttgatcgtca tttcggattc tttcttttgc
     2701 tctgctatta ttgagagaaa gtggactgcc attcataaat tctgcagccc tttccattca
     2761 aacgtgaaat caataagctc atcaggagca tctcaaacaa tatgaagacg gacatcgtct
     2821 ctgccgtgcc tcttttcact aattgcgtaa ctgtcatgtt ttggattatt taattctttc
     2881 agcgtaaata tctcctcaaa gactctatta agccgactct tatttccttt tacagcgaat
     2941 aaataatcac atctctgttt ttatatcttc tctgcaatat ctttctggca agccatcgca
     3001 tcagttatga ttatttttcc tttaataccc atcattttag aaagttcatg aataactgtg
     3061 aacgcattag gtttctcatc cgtcttgatc tatccgagga ccagactgtg cattgctgaa
     3121 acgcactaat gacatgaacc actcctctgc gacgactctt gtcataagtg tgccgaagtg
     3181 ttttaccatc aattgcgatg acgtcgttat catctggaaa atggcagaca tgtattcagt
     3241 taacaaagca ctcgtgaaat attgcaggac tgatgcagga tacaactttg gctataatat
     3301 cgtaaacagg gataataaaa atctattgtc tcaattattt taagttccat
//
LOCUS       DAZFTN010000257         3344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFTN010000257 DAZFTN010000000
VERSION     DAZFTN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            22..696
                     /locus_tag="ACHKV2_005165"
     CDS             22..696
                     /locus_tag="ACHKV2_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6942851.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNN
                     VVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHGN
                     YISDKI"
     gene            712..3192
                     /locus_tag="ACHKV2_005166"
     CDS             712..3192
                     /locus_tag="ACHKV2_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6942852.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            3203..>3344
                     /locus_tag="ACHKV2_005167"
     CDS             3203..>3344
                     /locus_tag="ACHKV2_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO6942853.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SFS"
BASE COUNT          981 a          621 c          818 g          924 t
ORIGIN      
        1 ataaatataa gaggcattaa gatgaaaggg ttattatctt tactcatctc ttctatgata
       61 ttccccgtgc atgccggaat ggttattttc gggacccgta tcatttaccc ggcagagcag
      121 aaagaggtca tggtacaact gatgaatcag ggaaaccgct cttcgctgat gcaggcgtgg
      181 attgatgatg gcgacacgtc tctgccgcca gaaaagattc aggttccttt catgttaacg
      241 ccaccagtag tgaaagtagc ggccaattct gggcaacaaa taaaaattaa aatcatgcct
      301 aataaattac ccagtaataa ggaaagtatt ttttatctta atgttctgga tattccaccg
      361 aatagcccag aagatgaagg aaagaatgcg ttaaaatttg ccatgcaaaa cagaatcaag
      421 ttgttttacc gacctgctgg tattgctccg gtaaataaaa cgacttttaa aaaattacga
      481 gtgagtcgta gcggtaataa cgtagtcata aaaaatgact cgccaaattg ggtaacgatt
      541 tctgatgtta aggctaataa cgtcaaagtg aattatgaaa caatcatgat tgcaccacaa
      601 gaaagtcaga atgttagtga aaaaaataat aatgcgaatg gctggcaact gaccattatc
      661 gatgatcacg gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga
      721 atgaccccgc ttgcatcagc aatagtcgcg ttattgctcg gcattgaagc acatgcagca
      781 gaagaaacct ttgacaccca ttttatgatg ggaggaatga aaggtgagca agtaacaaat
      841 ttgcgtcttg atgataatca gcctttacct gggcagtacg acattgatat ttatgtcaat
      901 aaacaatggc gtggaaaata tgagattatc gtcaaagata acccacatga aacttgcctg
      961 acgcgtgaaa ttgtaaagcg gttaggtata aatagcgata attttgcacg cgaaaatcag
     1021 tgtttaacat ttgagcaact tgttcagggc ggaagctact cctgggatat tgggattttt
     1081 cgtttggatc tcgctgttcc gcaagcctgg gttgaagagc tggaaaatgg ttacgttccg
     1141 ccagaaaact gggagcgggg aatcaatgcg ttttatacgt cttattacgt aagtcagtat
     1201 tacagtgact ataaagcctc gggcaatagt aagagcactt atgtgcgttt taacagtggg
     1261 ctaaatttgc tgggatggca gctgcattca gatgccagtt ttagcaaaac ggataataat
     1321 cctggtgagt ggaaaagcaa cacgctgtat ctggagcatg gattttctca aattctcggt
     1381 acgctgcgta ttggtgatat gtacacctca gccgatatat ttgattccgt tcgcttcacc
     1441 ggggttcggt tatttcgtga tatgcagatg ttgccaaact cgaagcaaaa ttttacccca
     1501 cgggtacagg ggattgccca gagtaacgcg ttggtaacta ttgaacagaa cggttttgtc
     1561 gtttatcaga aagaggttcc accaggtccg ttttcgatta gtgatttgca gttagcgggc
     1621 ggaggggcgg atcttgatgt tagtgttaag gaagccgatg gttctgtgac tacgtatctg
     1681 gtgccttatg cggcagtacc taatatgctg caacctggtg tatcaaaata tgattttgcg
     1741 gcaggccgta gtcatattga aggtgcgagc aagcaaagtg attttgtcca ggcgggttat
     1801 cagtatggtt ttaataattt attgacgctg tatggcggca cgatggttgc taataattac
     1861 tatgccttta ccctcggaac aggttggaac acgcgtattg gcgcaatttc agtcgatgca
     1921 actaaatcgc acagtaagca agacaatggt gatgtgtttg acggacaaag ttatcaaatt
     1981 gcctataaca aatttttgag ccaaacatca acacgttttg gtctggcggc ctggcgttat
     2041 tcgtcgcgtg attaccgcac atttaacgat tatgtgtggg caaataataa agataattat
     2101 cgtcgtgata aaaacgatgt ctatgatatt gccgattatt accagaatga ttttggtcgc
     2161 aaaaatagct tttccgccaa tatgagtcag tcattgccag aaggctgggg atctgtgtca
     2221 ttaagtactt tatggcgtga ttactggggg cgaagcggca gtagtaaaga ttatcaactg
     2281 agttattcca ataactggcg gcggataagc tataccctcg cggcaagcca ggcgtatgac
     2341 gagaaccacg ccgaagagaa acgctttaat atttttatat cgattccctt tgactggggc
     2401 gatgacgtta cgacgccgcg acggcaaata tatatgtcta actcaacgac ctttgatgat
     2461 cagggttttg cttcaaacaa taccggatta tcgggaaccg ttgggaaccg cgatcagttt
     2521 aactatggta tcaacttgag ccatcaacat cagggaaatg aaacgacagc tggggccaat
     2581 ttgacctgga ccgcaccggc cgcaacagtg aatggcagct atagtcagtc gagtacttat
     2641 cgacaggtcg gagccagtgt ttcaggggga ctggttgcct ggtctggtgg cgttaatctg
     2701 gcgaaccgtc tttccgaaac gtttgctgtg atgcatgcgc cgggaatcaa agatgcttat
     2761 gtcaatgggc aaaaatatcg tacaacaaac tgtaatggtg tggtggtgta cgacggactg
     2821 acaccttatc gggaaaatca cctgatgatg gatgtgtcgc aaagcgatag cgaaacagaa
     2881 ttacgcggga accgtaaaat gaccgcccct tatcgcggcg cggtcgttct ggttgatttt
     2941 gataccgatc agcgtaagcc ctggtttata aaagcattaa gatccgatgg acagccatta
     3001 acgtttggtt atgaagtcaa tgatatgcat ggtcataaca ttggtgttgt cggccagggc
     3061 agtcagatat ttattcgcac caatgaaata ccgccagcgg ttaatgtggc aattgataag
     3121 caacaagggc tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag taaaaattat
     3181 atctgtcggt gagaggtgaa atatgatgtt cagaaataga atattactaa tatttatatt
     3241 gtgggctaat tttacctggg ctgggtgtcg tactactgca tcattaaata ttacagatgg
     3301 tattaatgtt ggggagattt tagcgaatga aacttccttt agta
//
LOCUS       DAZFTN010000258         3312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFTN010000258 DAZFTN010000000
VERSION     DAZFTN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..171
                     /locus_tag="ACHKV2_005168"
     CDS             <1..171
                     /locus_tag="ACHKV2_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942854.1"
                     /translation="DLLYNESRPGGLVLPSSFWRAVAGVKGLEDFEVRSPLKSVMAGD
                     TELLTVGEITWL"
     gene            162..722
                     /locus_tag="ACHKV2_005169"
     CDS             162..722
                     /locus_tag="ACHKV2_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO6942855.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            722..1633
                     /locus_tag="ACHKV2_005170"
     CDS             722..1633
                     /locus_tag="ACHKV2_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942856.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNTWRTVSSV"
     gene            complement(1668..2189)
                     /locus_tag="ACHKV2_005171"
     CDS             complement(1668..2189)
                     /locus_tag="ACHKV2_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6942857.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            2546..2731
                     /locus_tag="ACHKV2_005172"
     CDS             2546..2731
                     /locus_tag="ACHKV2_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942858.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISTLKGFSTSF"
     gene            2745..3305
                     /locus_tag="ACHKV2_005173"
     CDS             2745..3305
                     /locus_tag="ACHKV2_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6942859.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
BASE COUNT          853 a          792 c          826 g          841 t
ORIGIN      
        1 gacctgcttt ataacgaatc gcggccgggt ggacttgtat tgccttcatc attctggcgg
       61 gctgttgcag gggtgaaagg actggaggat tttgaagttc gcagtccgct gaagtccgtg
      121 atggccggag atacagagtt gctgaccgtg ggggaaatca catggctgta accctgaccc
      181 cgcatcagcg cgccctgttg cagttgctgc ctgacgggct ggcatgggat aagcggccgt
      241 catccgttct tgcggctttg tgcctgggcc tcagtcattc cacggagcgt gtttcctgga
      301 ccggtaacca gatgctggca gaacgttttc ctgattcatc ccgtctgctg ctggaagact
      361 gggagcgtta tctggggtta ccggaatgtg atatgaccgg cgcaaccatt caggaacgtc
      421 agcgttatgc agggaataaa taccggatga aaccctctct taaccgtgaa ttttatatcc
      481 ggtttgcggc agagtttggt tatgaaatag atattcagcc atcaccggat tcacagtggg
      541 tcagtattgt cacgataaac agtgaaaccg gctaccggaa tatgaatgtg ctggatgata
      601 ttctcacccc gctgcgtatt tatgagggtg gtgcgctgga atgtattctg aaccgttata
      661 agcctgcatg gcagacgttt atttacgtgt atgcaaacag tcacaaagag gagaatattt
      721 aatgttccat gttgataata attccggcgt ggcgaatatg cctgaactgg ctccggcgca
      781 gagtaatacc accacctggt ttaccgaagg tgacggacaa aaaggtatca gctggattgg
      841 tcaggactgg ctgaatattc tccaggccga actgctgaat attctggctg aagccagtat
      901 tcagccggat aaggcgcagt taaaccagct tacgctgtcc attaaagcca ttatcgctgc
      961 gaatgccttt tcccggaaaa acaacctgaa agaaattgct gatgccggtg cggaagccca
     1021 gcgtctggcc cgtggttatc ttggtctggg gacgcttgcc acaaaaaaca gtcttggtcc
     1081 cgctgacgtt aatgccctgg cgaaggatca gaaccttgcc gacctggaga atgcggaaac
     1141 cgcccgtaat aatctggatg tttacagcag gagcgaaggt gataaccgtt acctgcgcag
     1201 ggaccagaat ggcgcggata tccaggacaa aggaaccttt atcgataacc tcggtttacg
     1261 ggaaacggta aataaggctg ctgatgccct gccatcggct ggaaccgccg tggcagcaaa
     1321 cagactcgcc accccaagaa atattaatgg tgttcctttt gacggaacgc tggatatcaa
     1381 catcacgtca ggaatgacgc agtcaaccgc agatggccgg tatgtacaga atgttcagct
     1441 tggagcacag agctatcatt cacccggagg caatgaaatg tcatggaatt acagcgcacc
     1501 ttccggttgt atgctttccg gtattaacgt gcaggaaacc ggctcccggt ctgcggacaa
     1561 tatcggtggg gtctattatc gcccggttca gatttatatt aataatacct ggagaacggt
     1621 aagttcagtg taaaccacag aaaagggtgc tgaatgcacc ctctgaatta ttccggtttt
     1681 tccggccagg gaatcgcctg gtaatgcccc tcactggcaa tatcattaaa gtgcatttct
     1741 ttaagctgtc ggatatacgc catccagcgc gtcagctgta atttattttc atcgctaatc
     1801 atgtccagtt ttaattctgt tttccagtca tccacggttt cgttggcttc ctggagcagc
     1861 gatgagcgac gcttttcagc aaccgtcttc cagtttaccg gcacctttga tatcaggcca
     1921 tcagaaaact gccagttacc gtcaatattc acaccttccg gcagttcgtc cacttcaaca
     1981 acagtaaaac ctgccggata taaagcagag acatcttcag caacagagca gataataccc
     2041 gttacaggcg aaatacagag tttgtatttc tttgtaaaaa gcgggataga ttcataaaaa
     2101 tcccttccgt cctcactctg gaaatactga acatcctcac catagggttt gtctttcggg
     2161 taatatcgtt tgacattaat cagttgcata aacatcacca tgtaaatcaa tgataaaaac
     2221 aggaataata ctatcgccat atacaggcaa tgcttgtctg aaataacgca tatttcagat
     2281 gctggctgct gtgtgctatg cccccgttaa ttaaatactt gcagcggata cccaatgccc
     2341 attaatacaa tattgtatct gtctgtaata aacacggtca tcaccaccac gcatttccca
     2401 gtctccgtca gtcttaaaac cggtaatcac atttcctgat gacagggagt aattatattc
     2461 cagtccgccg cttaatgcct gcgctcccag tcttacccct gtaacataac gctgattggc
     2521 cgttgattcc ggcattcctg acgtaatgtt tatatcctgc gtgccgtcaa aggcgacacc
     2581 attgattctg cgggcatttg ccagcctgct tgctgcaaca gccgtaccgt ttgatggcag
     2641 ggcattcgcg gccctgttta ccgtatcccg taaaccgagg tttcagggat gccgtatatc
     2701 tactttaaaa ggcttttcca cctcttttta aggaaggtaa atacatgctg attggctata
     2761 tacgcgtatc aacaaatgac cagaacaccg gtttacaaag aaatgcgctg gaatgtgcag
     2821 gatgtgagca gatttttgaa gacaaaatga gtggtaaaac gacacagcgg ccgggactga
     2881 agcgggcact gaagaaacta aaacccggtg acacgctgat ggtctggaaa ctggacaggc
     2941 tggggcgcag tatgcgccac cttgttgcgc tgacagatga attacagcaa caggggataa
     3001 attttcgtag tcttactgac aacattgata cgtcaacccc catggggcgc ttcttttttc
     3061 acataatggg tgcgctggca gaaatggagc gtgaattaat tgtcgaacgg acacgggctg
     3121 gtcttgcagc tgcccgtgaa aggggacgaa ccggcggcag accccgccgt ctcacgcagg
     3181 aacaatacga gcaggtcggg cgtttactgg ccgggggtgt cagtcgaaga caggtggcac
     3241 ttatttttga tatcggtctt tcaacccttt atcgttatta tcctgcgagc gtttcggaaa
     3301 aataagacgc tc
//
LOCUS       DAZFTN010000259         3275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFTN010000259 DAZFTN010000000
VERSION     DAZFTN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            232..1164
                     /gene="rfaD"
                     /locus_tag="ACHKV2_005174"
     CDS             232..1164
                     /gene="rfaD"
                     /locus_tag="ACHKV2_005174"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO6942860.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHKV2_005175"
     CDS             1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHKV2_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO6942861.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHKV2_005176"
     CDS             2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHKV2_005176"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO6942862.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3213..>3275
                     /locus_tag="ACHKV2_005177"
     CDS             3213..>3275
                     /locus_tag="ACHKV2_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942863.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRA"
BASE COUNT          830 a          801 c          862 g          782 t
ORIGIN      
        1 cattcaaatg cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac
       61 atgcaaaacc aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt
      121 agcatccttg cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca
      181 gacattcgtg tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc
      241 gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc
      301 atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg
      361 gatctgaata tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc
      421 gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag
      481 tgggacggca agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac
      541 tgcctggagc gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc
      601 acctccgact ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca
      661 aaattcctgt ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt
      721 ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc
      781 gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt
      841 agcgagaact ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg
      901 ttcctggaaa atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc
      961 caggctgtag ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg
     1021 ttcccggata agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg
     1081 cgcgcggcgg gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg
     1141 gcctggctga atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct
     1201 tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc
     1261 caggcgataa tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg
     1321 gaagttaacg aagctattcc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc
     1381 cgcaaactgg gtcatagtct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac
     1441 tccttcaaat ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc
     1501 ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg
     1561 ctaatggtgg aacgctatgt cgcgctggcc tatgacaaag gcattatgcg cacagcacaa
     1621 gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat
     1681 acctgtaatc aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg
     1741 gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg
     1801 attgatgaag gttatcaggt ggttctgttt ggctctgcga aagatcatga agcaggcaat
     1861 gagattcttg ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa
     1921 acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat
     1981 tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtccg
     2041 agtagcccgg acttcacacc gccgctatcc cataaagcgc gcgtgatccg cctgattacc
     2101 ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac
     2161 attactcccc agcgcgtact gaaagaactc aacgcgctat tgttacaaga ggaagcctga
     2221 cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc
     2281 gcactcactg atgcccagca ggcaatccca gaaattaagt ttgactgggt ggtggaagaa
     2341 gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata
     2401 cgtcgctggc gtaaagcctg gttctcggcc cctataaaag ctgaacgcaa agcgtttcgt
     2461 gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc
     2521 gcggcgctgg ttacgcgtct ggcacatggc gtaaagcatg gcatggactg gcaaaccgct
     2581 cgcgaacctt tagccagcct gttttacaat cgtaagcatc atattgcaaa acagcagcac
     2641 gccgtagaac gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc
     2701 cagggcgatt atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa
     2761 tatgccgtat ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg
     2821 cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg
     2881 ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg
     2941 ccgaagatga gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg
     3001 gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat
     3061 ggaccaaccg atccgggatt aattggtggg tatggggaga atcaagtaga gtgtcgttct
     3121 acaagtatgt ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa
     3181 ataataacca ataagttgac atcggagata agatgacctc aacattattt ttctctctcg
     3241 agaaaaaaaa ctggatagcg tactggaaca gagct
//
LOCUS       DAZFTN010000260         3274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFTN010000260 DAZFTN010000000
VERSION     DAZFTN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..820
                     /locus_tag="ACHKV2_005178"
     CDS             77..820
                     /locus_tag="ACHKV2_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6942864.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYSISKVAKVCGYASVSYFT
                     YVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            complement(862..1296)
                     /locus_tag="ACHKV2_005179"
                     /pseudo
     CDS             complement(862..1296)
                     /locus_tag="ACHKV2_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1783..2964
                     /locus_tag="ACHKV2_005180"
     CDS             1783..2964
                     /locus_tag="ACHKV2_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6942865.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            2968..3274
                     /locus_tag="ACHKV2_005181"
                     /pseudo
     CDS             2968..3274
                     /locus_tag="ACHKV2_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          896 a          621 c          760 g          997 t
ORIGIN      
        1 tttatactga agtttggcaa agtgaacttt atatacatat acttcatcct ggtttcagtt
       61 aaattgggtg gatgatatgg caactacatg ttcagttata ttgattttgg agtcctttga
      121 tgtttatttc ggaaaagaga gtgtgtttct ggagagaggt tcatctgtac ttgtcgactc
      181 tagctctaga gattttttcc tgacatatcc tgaaagagtg atagtggcgg attttggcgc
      241 tgagtttatt agtcgctatt tgaaagctaa taacttaagg gatatttctg attgtaggga
      301 atatccatct tatttaaaaa taaactttgc tgacttcagt ttaattaaag gattaattag
      361 ttgggctaat cactgtgctg aatacataga aatttttgat gagtctattg cttttacatg
      421 tctctctgca ttttcttctg aaaaacaatt tggagtattt ctgtttggat gtttgaaaag
      481 cacaggggct aaagttaaaa cgattattca tacggattta tctgcaccat ggcgtcttaa
      541 ggatatatca tcaagattat atctcagcga aagtttacta aagaggaaat tgaaagaaga
      601 gggggtatca ttcagtaaga tcatacttga tgagaggatg caaatggctg aatatttact
      661 cagcactcgt tgttattcta ttagtaaagt agctaaggtc tgtggttatg ccagtgtctc
      721 atactttact tatgtattta gacgttattt tggtgtttct ccaagtcaat actctcagag
      781 gagttcagaa agtaaaattc ttactcacca gggaatctga tcattgttct tgccccctta
      841 tttccagaca gggggtgtat cttaagttaa cgttacccgc tgacgtcgat attctcgcgg
      901 agagcgataa cccaacgcac tgtgcggatg gttttcattg taatgttcga tcgccactgc
      961 aagattatgc aatgccgttc ttacattcgg tttcggcatg aacgcgatat agtcttcctt
     1021 catcgttttc acgaaccttt ctgcaattcc attactctga ggactgctga ttgccgttgt
     1081 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgcgctc tatacgctga
     1141 accgttatct gtcagccact ggatggactg ttccggtact ttatcgccaa agcgtttttc
     1201 tatcgctcct aacatgacat cctgcactgt cgctttatcg taacctcccg tgcttgcggc
     1261 ccagtctatg gcttcacgat cgcaacagtc cagggcgatt catctgggcc aatcctcgct
     1321 cataacaggc attcacttca gtcatggcag aaaggtatgc atgctggaga agtcgtgaaa
     1381 gaaaagaaga ctgctgcgcc gtttgtcgtc acgtttatct tcattggcta tgcaagtcgt
     1441 aatacaaggt gggacaaaac tgagacacat aaggcctcgc aatggcttgc aaggctttac
     1501 atgttttgat gtggtgggac gtgtgagcgc agtgttgatg gggtaatgct ttgaattaga
     1561 agcggattct tataattcgt aatgcgaagg tcgtaggttc gactcctatt atcggcacca
     1621 tctcaacttc ctcaaacgtc cgtattagtc cataaaatct ctgatttata acatttttta
     1681 ttcttttaag tccatagtag tccgtaacca tccagtagaa tccggtactg aatgtgtata
     1741 ggattgtgta tatgttcctg ttcggtctgg attcctatac acatgccttt aaacgatatg
     1801 cagattcgcc gcgctaagcc tgaagctaaa gcctatacat ttggagatgg gctagggttg
     1861 tcattactta tagaacctaa tggaagcaag agttggcggt tccgctatcg ctatgccggc
     1921 aaacccaaaa tgatctcgct tggtgtttac ccaacgatca cccttgccga tgctcgttcc
     1981 cgtcgtgatg aagctcgaaa acttgtggca gaaggaaaga accctagtga ggttcgaaaa
     2041 gagcaaaagc tagctatgca aacagagtca gagaacgcct tcgaaaagat agccagagag
     2101 tggcatcaac ttaaatctgc taaatggtcg gcgggatatg catcagacat catggaagcg
     2161 tttaagaacg acatttttcc ttatgtcgga acaaggcctg tgggagagat taaaccgcta
     2221 gagctgctga acgttctgcg taaaattgag aaacgtggtg cgttggagaa aatgcgcaaa
     2281 gtgcggcagc gttgctccga agtgtttcgc tacgcaattg caacgggtag ggcggagtac
     2341 aatcctgcgg ctgatctctc cagcgctctc gaagtacacc aatccaatca tttcccattc
     2401 ctaaaagctg atgagatacc tgattttcta cgtgccttag agggttactc cgggagtaag
     2461 cttgtccaga tagccacgaa attactgatg attacgggtg tgagaaccat cgaattacgc
     2521 gcggcattat ggcaagaatt tgatctggat aacgctattt gggaaattcc tgctgaaagg
     2581 atgaaaatgc gtaggccaca tcttgtgccc ttatcatctc aagcggtaga tttactcaat
     2641 gaactcaaga tcatgacagg gaactatcgt tatgtttttc cagggcggaa cgatccgaat
     2701 aggccaatga gcgaagcgag tataaatcaa gccattaagc gtattgggta tggaggaaaa
     2761 gtcactggac atggttttcg tcataccctt tctacaatcc tgcatgagca aggttttgag
     2821 agtgcttgga ttgaaatcca gttggctcat gtagataaaa attctattag ggggacttat
     2881 aaccatgctc aatattttag tggaaggaag tctatgatgg actggtacag taatttgata
     2941 tttgaaagac taaaaaggag ttaatttgtg tctcaaccac tttcggaaat attgacatgg
     3001 gatgatgaac atgggaggta tttgtccatg attggcttat tgtctgtaaa tcagatgatt
     3061 acccgtggag cgaacgtttg ggaggagctg gagataaagg tagagacgtt gttggatata
     3121 aatcggatcc taacgtagaa ggttattctt gggataatta tcaatgcaaa ctgtacaaaa
     3181 aaagtttagg gttctctgat gttgtagttg agtttggaaa acttatctat tttactctga
     3241 atggtgatta tcccatccct cagaagtact tttt
//
LOCUS       DAZFTN010000261         3235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFTN010000261 DAZFTN010000000
VERSION     DAZFTN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            683..1849
                     /gene="sopA"
                     /locus_tag="ACHKV2_005182"
     CDS             683..1849
                     /gene="sopA"
                     /locus_tag="ACHKV2_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO6942866.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            1849..2820
                     /locus_tag="ACHKV2_005183"
     CDS             1849..2820
                     /locus_tag="ACHKV2_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO6942867.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
BASE COUNT          787 a          693 c          797 g          958 t
ORIGIN      
        1 caatgcgcct ctcatacatt gagaaaaaga aaggccgcca gacgactcat atcgtatttt
       61 ccttccgcga tatcacttcc atgacgacag gatagtctga gggttatctg tcacagattt
      121 gagggtggtt cgtcacattt gttctgacct actgagggta atttgtcaca gttttgctgt
      181 ttccttcagc ctgcatggat tttctcatac tttttgaact gtaattttta aggaagccaa
      241 atttgagggc agtttgtcac agttgatttc cttctctttc ccttcgtcat gtgacctgat
      301 atcgggggtt agttcgtcat cattgatgag ggttgattat cacagtttat tactctgaat
      361 tggctatccg cgtgtgtacc tctacctgga gtttttccca cggtggatat ttcttcttgc
      421 gctgagcgta agagctatct gacagaacag ttcttctttg cttcctcgcc agttcgctcg
      481 ctatgctcgg ttacacggct gcggcgagcg ctagtgataa taagtgactg aggtatgtgc
      541 tcttcttatc tccttttgta gtgttgctct tattttaaac aactttgcgg ttttttgatg
      601 actttgcgat tttgttgttg ctttgcagta aattgcaaga tttaataaaa aaatgcaaag
      661 caatgattaa aggatgttca gaatgaaact catggaaaca cttaaccagt gcataaacgc
      721 tggtcatgaa atgacgaagg ctatcgccat tgcacagttt aatgatgaca gcccggaagc
      781 gaggaaaata acccggcgct ggagaatagg tgaagcagcg gatttagttg gggtttcttc
      841 tcaggctatc agagatgccg agaaagcagg gcgactaccg cacccggata tggaaattcg
      901 aggacgggtt gagcaacgtg ttggttatac aattgaacaa attaatcata tgcgtgatgt
      961 gtttggtacg cgattgcgac gtgctgaaga cgtatttcca ccggtgatcg gggttgctgc
     1021 ccataaaggt ggcgtttaca aaacctcagt ttctgttcat cttgctcagg atctggctct
     1081 gaaggggcta cgtgttttgc tcgtggaagg taacgacccc cagggaacag cctcaatgta
     1141 tcacggatgg gtaccagatc ttcatattca tgcagaagac actctcctgc ctttctatct
     1201 tggggaaaag gacgatgtca cttatgcaat aaagcccact tgctggccgg ggcttgacat
     1261 tattccttcc tgtctggctc tgcaccgtat tgaaactgag ttaatgggca aatttgatga
     1321 aggtaaactg cccaccgatc cacacctgat gctccgactg gccattgaaa ctgttgctca
     1381 tgactatgat gtcatagtta ttgacagcgc gcctaacctg ggtatcggca cgattaatgt
     1441 cgtatgtgct gctgatgtgc tgattgttcc cactcctgct gagttgtttg actacacctc
     1501 cgcactgcag tttttcgata tgcttcgtga tctgctcaag aacgttgatc ttaaagggtt
     1561 cgagcctgat gtacgtattt tgcttaccaa atacagcaat agtaatggct ctcagtcccc
     1621 gtggatggag gagcaaattc gggatgcctg gggaagcatg gttctaaaaa atgttgtacg
     1681 tgaaacggat gaagttggta aaggtcagat ccggatgaga actgtttttg aacaggccat
     1741 tgatcaacgc tcttcaactg gtgcctggag aaatgctctt tctatttggg aacctgtctg
     1801 caatgaaatt ttcgatcgtc tgattaaacc acgctgggag attagataat gaagcgtgcg
     1861 cctgttattc caaaacatac gctcaatact caaccggttg aagatacttc gttatcgaca
     1921 ccagctgccc cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc tcgcggtaat
     1981 gccattactt tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt gctccggggt
     2041 gatagtgttg agaagacctc tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt
     2101 actgaggacg cactggatga tctcatccct tcttttctac tgactggtca acagacaccg
     2161 gcgttcggtc gaagagtatc tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa
     2221 gctgctgcac ttaccgaaag tgattatcgt gttctggttg gcgagctgga tgatgagcag
     2281 atggctgcat tatccagatt gggtaacgat tatcgcccaa caagtgctta tgaacgtggt
     2341 cagcgttatg caagccgatt gcagaatgaa tttgctggaa atatttctgc gctggctgat
     2401 gcggaaaata tttcacgtaa gattattacc cgctgtatca acacagccaa attgcctaaa
     2461 tcagttgttg ctcttttttc tcaccccggt gaactatctg cccggtcagg tgatgcactt
     2521 caaaaagcct ttacagataa agaggaatta cttaagcagc aggcatctaa ccttcatgag
     2581 cagaaaaaag ctggggtgat atttgaagct gaagaagtta tcactctttt aacttctgtg
     2641 cttaaaacgt catctgcatc aagaactagt ttaagctcac gacatcagtt tgctcctgga
     2701 gcgacagtat tgtataaggg cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca
     2761 actgagtgta tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc agcaccctga
     2821 tgcgaccacg ttttagtcta cgtttatctg tctttactta atgtcctttg ttacaggcca
     2881 gaaagcataa ctggcctgaa tattctctct gggcccactg ttccacttgt atcgtcggtc
     2941 tgataatcag actgggacca cggtcccact cgtgttgtcg gtctgattat cggtctggga
     3001 ccacggtccc actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta
     3061 ttgtcgatca gactatcagc gtgagactac gattctatca atgcctgtca agggcaagta
     3121 ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct
     3181 gtggattgct gctgtgtcct gcttatccac aacattttgc gcacggttat gtgga
//
LOCUS       DAZFTN010000262         3203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFTN010000262 DAZFTN010000000
VERSION     DAZFTN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            142..774
                     /gene="yqeH"
                     /locus_tag="ACHKV2_005184"
     CDS             142..774
                     /gene="yqeH"
                     /locus_tag="ACHKV2_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6942868.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1108..1917
                     /gene="yqeI"
                     /locus_tag="ACHKV2_005185"
     CDS             1108..1917
                     /gene="yqeI"
                     /locus_tag="ACHKV2_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6942869.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRG"
     gene            1914..2393
                     /gene="yqeJ"
                     /locus_tag="ACHKV2_005186"
     CDS             1914..2393
                     /gene="yqeJ"
                     /locus_tag="ACHKV2_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO6942870.1"
                     /translation="MIIKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIFN
                     DQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTSK
                     KVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(2426..2506)
                     /locus_tag="ACHKV2_005187"
     CDS             complement(2426..2506)
                     /locus_tag="ACHKV2_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942871.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(2542..2967)
                     /gene="yqeK"
                     /locus_tag="ACHKV2_005188"
     CDS             complement(2542..2967)
                     /gene="yqeK"
                     /locus_tag="ACHKV2_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO6942872.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT         1107 a          560 c          546 g          990 t
ORIGIN      
        1 ttttaaaatg tcttaatcag aattatagca aatacagatt aatcgcaatt tttcccagca
       61 aaattacaaa atcatggtgt attgacatca tcaatcacca atgaaattat gcaattatat
      121 acggataggg aggttcttaa catggggctt tgtagtcgtt ataaaagtct tacatgcaat
      181 agttgctcaa tgcattgcca aataatgcca gaagagtcac cgcgtttgca gtactgcgca
      241 aactcgtgtt tttgtatgtg gaccgaagaa agttcatatt ttaatcgtgg tgtggtagaa
      301 ggtatcttaa caaaaaacca caatgcgaga ttaagcggat atatttttgt cgatttttca
      361 gtaagttttt tacgtctatt ccttgaaaaa gactggattg actatcttgc aagtactgac
      421 atggggattg ttttagtcag cgaccgcaat atgcaatcgt tagctaatta ctggcgaaaa
      481 cacaactctg caatatctgc tgtcatatat aatgatgatg gtcttgatgt tgccaatgaa
      541 aagatcagac aactatttat tggtcgttat ttatcattta ccagaggtaa cacattaact
      601 cagatggaat tcaccataat ggggtatatg gtttctggtt ataatccata tcagattgct
      661 gaagttctgg atatggatat ccgtagcatc tacgcgtaca agcaacgaat cgaaaagaga
      721 atgggtggta aaataaacga attatttatt cgttcacatt cggtccaaca ttgatactca
      781 acaatctgcc agaatcatac tctgtttata cgggcattta ttgaaatgcc cgtattttat
      841 aaataggccg tcccctcaag agtaaacacc atagtattta aaaccatcac acgaaaaatt
      901 cagaagcatt acgaaatatg aacttacaaa caatagtggc ataaatgtta accatgttaa
      961 tttacataaa attttacgtt gcgacattaa agcctcattt caatcatcat gataaatata
     1021 aaattaatat atatttatgc cgtaaatacc aatatactta gcaaactatg tgatttccat
     1081 ttcgattgat ttagtgttta ttgacgtatg tactggatta ttaacgataa tatcgagttc
     1141 tggcctgagc accgaaaatt aatatcggta cataacgccg atcttaacgt cgttctgaca
     1201 acgccagcca gtcgttgttt atcactgcta cttgaagctt ttcctgatgt ggttgcacaa
     1261 caagattttt tcaccagagt ctgggaagaa gaaggtatgc gtgtgcctac taacacgtta
     1321 tatcagaaca tatccattat cagacgcgga tttcgcgctg ttggtgatac tacccactcg
     1381 cttattgcaa cagtgccgag aagaggattc aagatccata atgacattaa catacaaaat
     1441 catgtaataa actcatcaac agacgcacat acacacaatg ccccacctgc cataaaagtt
     1501 aatgcgggat acaaagagag cattggtggc gcaaagaatt tcaataacaa aatcctcaaa
     1561 catataaaaa gttatctaat tatgttgagc gcatttgtca taggcgcata ttctgcatat
     1621 tggctatgga ataataatca accgaagcca tttttcaagg attacaagac tgttgcagaa
     1681 attaatggct gccattttaa tgtaacagaa gatacaattg atgggttgaa agagttcgat
     1741 aaatataaaa cacggatact ggattcgggg attaactgca aaaaatatcc gtggttatac
     1801 ttccctcttg caaaatcctc accggggatg attgttatgg catgcaataa aaactataac
     1861 caacatgaag tggcaggctg tttaaccctg tcttaccgtg aggttaatcg tggttgatta
     1921 taaaaaaaaa cttattattt attttagttt ttatcagtgg gtttattctc ttcactgtct
     1981 attcttacac tgcagaaaaa atgatatata acgaaacatg cactgcaaat tgggtaattt
     2041 ttaatgacca gggacgagca aatcttacca tagactttat gtataacaaa aaaaataaaa
     2101 caggaactgt tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt aaatcaatac
     2161 ggcgaaatat tgaatacaca tgggttgaaa actatgacac agcccattta acatcaaaga
     2221 aagttaataa gtttgagatt atggatcaag tcgatgatga tagacttgca gaacttattc
     2281 ctgattttta tgtctttcca gaaaaaagtg taagctataa tatactaaag caaggtaagc
     2341 atgcttttat tttgagcatt ggtaacagag caataatgca ttgtgcaagg taaactaaaa
     2401 tagataacaa aatgatggaa cctcgttaat cataaaacaa taaaattaaa tattctcgca
     2461 gtatatggca gtctaaagca tcaaagtttt gatcaacatc tttcatttta gacatctcct
     2521 tacaatgtaa aatatcatgt atcaacaaca tctggtgcaa atccatgagt cgaactcgac
     2581 attcatctta gctcgattat tattccttcg tacagtcgat gtaaacaata cagaaagagg
     2641 attattaaga accagtttac tcagtgaatc aatagaggaa aggactaacg tttctttaaa
     2701 agaattgatt tcatcatctg ttaaactaaa ctcatcattg acagatcgtg agatataact
     2761 gtttttaact ttactcttta cgttgacttt attgacagaa ttaacattca caaatcttga
     2821 atttaatgcc catgatgttg tttgcgtgag aaccttctca gcattaagga atttttttaa
     2881 cggaatcctt ggtttctttt tcgagtccga atttacaata tcatgcatat agaccatttc
     2941 atgaatctgc gaaaactcaa tgtccattat atacctcaca tttttaccgt gactcgatgt
     3001 tactgttcaa taatcacctt ccatcaatac taaaattaat acccctaatg tgccgataac
     3061 aaatatagtc attctacgta acgtctccat aaggtgataa ccttgatcaa atgaatttcg
     3121 aatataataa aggaaatctt ttgagatatt tatcacgaat aaagctattt atgttaccaa
     3181 tatcatttcg agtacgcaca cta
//
LOCUS       DAZFTN010000263         3184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFTN010000263 DAZFTN010000000
VERSION     DAZFTN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..79
                     /locus_tag="ACHKV2_005189"
     CDS             <1..79
                     /locus_tag="ACHKV2_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942873.1"
                     /translation="VCREWSVTDNARYSDFSCTIEQVVN"
     gene            79..777
                     /locus_tag="ACHKV2_005190"
     CDS             79..777
                     /locus_tag="ACHKV2_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6942874.1"
                     /translation="MQNIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRSMV
                     ANFGGFLSINKLSQ"
     gene            complement(794..1048)
                     /locus_tag="ACHKV2_005191"
     CDS             complement(794..1048)
                     /locus_tag="ACHKV2_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO6942875.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            1158..1268
                     /locus_tag="ACHKV2_005192"
     CDS             1158..1268
                     /locus_tag="ACHKV2_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO6942876.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1571..2449
                     /locus_tag="ACHKV2_005193"
     CDS             1571..2449
                     /locus_tag="ACHKV2_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HFO6942877.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2503..>3184
                     /locus_tag="ACHKV2_005194"
     CDS             2503..>3184
                     /locus_tag="ACHKV2_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6942878.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTH
                     SAWRHRHWH"
BASE COUNT          826 a          690 c          896 g          772 t
ORIGIN      
        1 tgtgtgccgg gagtggagcg tcacggataa cgcccggtac agtgatttca gctgtacgat
       61 tgagcaggtg gtgaactgat gcagaatatt catgaagaaa gcctgaacga gtcggttaag
      121 tcagagcagt caccgcgggt ggtgctctgg gaaatcgacc tgacggtgca gggcggtgag
      181 cggtattttt tctgtaatga gctgaatgaa aaaggggagg cggtcacctg gcaggggcgg
      241 caatatcagg tatacccgat tgacggcagt ggctttgaga tgaacgggaa gggcagcagt
      301 gcccgcccgt cgctgacggt gtcgaatctg ttcggtctgg tcaccggaat ggcggaggac
      361 ctgcagagcc tggtgggggc cacggtggtc cgccgccggg tgtatgcccg ttttctggat
      421 gcggtgaatt ttgtggcggg caatccggaa gcggacccgg agcaggagct gagagaccgc
      481 tgggtggtgg agcagatgtc agagctgacg gccatgacag cctcgtttgt gctggcaaca
      541 ccgacggaga cggacggagc gctgtttccc ggtcgcatca tgctggcgaa cacctgtatg
      601 tgggattacc ggggagatga atgcggttat cacggtcctg cggtggcgga tgagttcgac
      661 aaccccacca cggatatccg taaggacaga tgcagcaagt gcatgcgcgg gtgtgagatg
      721 cgcagcatgg tggctaattt tggcggtttc ctttccatta ataaactttc gcagtaatgg
      781 attatgccca ccatcaggtg ggttttttat cttcgccgta catgctgctg agagtcttaa
      841 gaagagcctc cctaaatttt tcagattctt gttgagcaaa accttctatt gatcttggtg
      901 atttttcttc atcaatagca gcttgcaaga tcataactat ttctgaattt aatgagcgtc
      961 cgtttttcga tgctcttatg gtcaggtcgc gcttgaggct atctggcatt cttacgctat
     1021 atggggctat gtctctaacc ttcgtcatac ttacaccgtg ataatcacat tgatatcaca
     1081 gtgtattcaa aaaaactttg acttgatata gtcacagtga tacgtttgtg attcataata
     1141 actcacgcgg tggcgatatg gaaaaagaag ttagcagaat tttggtgaga atacctcagt
     1201 cgctaaaaga tgcgattaca ggaaaggcca aagaagaatg ccggtcgttt aactcggaag
     1261 tgatgtgatt gctggacagc ctgaagagag aggggataac ggtatgagta aagaatgttg
     1321 ttttttgcgg cattagcgaa tcagacgctg atcaaacata catttactct aaagaaacag
     1381 gtcggatgct gtgtagtgac tgcgtgtttg acatcataag atacaagtat cttggatgtt
     1441 ctgccagcat tagcaatata ggtgaagtgt acgaagggaa agatataact gatagagcag
     1501 aaagttgaag ccccaactgc tgtaacagtc agggcttcgt tatcaacaaa tcggcttagg
     1561 aaatattgac atgaaaagta tagcaaaggc acaaaacgat ttcactatct tcaaattcgg
     1621 cgacagtgaa atccgcgtca tcaacaagtg cggtgagccg tggtttgtag ctaaagatgt
     1681 ttgtgatgct ttagctttga ctaactcacg caaggcgctt actgcacttg atgacgatga
     1741 aaagggagta actttaagtt acacccttgg tggtgagcag aatctaagca ttgtgagcga
     1801 atcaggtatg tatacattgg ttctgcgctg ccgcgatgca gtcaataaag gttcagtccc
     1861 gcacaaattc cgcaagtggg taacagcaga agttctgcct tcaattcgca aacatggcga
     1921 gtatgtaaaa ggaaagaaaa ccactgttga ggaaagaaca ccgctacgcg atgcagtaaa
     1981 catgctggta ggaaagaaag gacttcgcaa tgacgatgca tacaatatgg ttcatcagcg
     2041 ttttggtatt gacagcattg atgagctttc aattgaacaa atcccgcttg ccgtagagta
     2101 catccacagg gtagtgcttg aaggtgaatt cattggcaaa caagagaaga gcaccaacga
     2161 gctttccgca aaagaagcaa acagccttgt atggctatgg gattatgcca accgctcaca
     2221 ggcattattc cgcgaactgt atccggcgct aaaacaaatt caatcgaact attccggcag
     2281 atgctacgac tacggtcatg agttctcgta tgttattgga atggcgagag acgttttaat
     2341 taatcacaca cgagatgttg atatcaatga gccagacgga ccaacgaatc tttccgcatg
     2401 gatgagactt aagaataaag aattacctcc ttcaccgcat cactactaac agattgccaa
     2461 cgaaatgacc cagcttcggc tgggttttta tcaggagttc tcatgctcta tagcaatata
     2521 ttggcgcacg cccggcgatg tgcaccagcg gagtcgtgcg gcttcgtggt gagaacgccg
     2581 gaaggggaaa gatattttcc ctgcgtgaat atctccggtg agccggaaga ttatttccgg
     2641 atgtcgccgg aggactggct gcgggcagaa atgcaggggg agattgtggc actggtccac
     2701 agccaccccg gtggtctgcc ctggctgagc gaggccgacc ggcggctgca gataaaaagt
     2761 gcactgtcct ggtggctggt ctgccggggg gaaattcata aattccgctg tgtgccacat
     2821 ctgacaggac ggcgctttga gcacggggtg acggactgtt acacgctgtt ccgggatgcc
     2881 taccatctgg cgggaattga tatgccggat tttgagcgtg aggatgactg gtggcgcaac
     2941 ggtcagaacc tttacctgga caatatggcg gtcaccggct tttaccgggt gcccctgtcc
     3001 tctgcacagg cgggcgatat cctgctgtgc tgctttggcg catcggtggc caatcatgcc
     3061 gccatatact gcggcaacgg tgaactgctt caccatctgc ctgaacaact gagtaaacgg
     3121 gagaggtatt ccgaaaaatg gcaacgacga acgcattcag cctggcgtca ccgccactgg
     3181 cacg
//
LOCUS       DAZFTN010000264         3137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFTN010000264 DAZFTN010000000
VERSION     DAZFTN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..606)
                     /gene="dsbA"
                     /locus_tag="ACHKV2_005195"
     CDS             complement(<1..606)
                     /gene="dsbA"
                     /locus_tag="ACHKV2_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO6942879.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVK"
     gene            complement(623..1609)
                     /gene="srkA"
                     /locus_tag="ACHKV2_005196"
     CDS             complement(623..1609)
                     /gene="srkA"
                     /locus_tag="ACHKV2_005196"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO6942880.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1686..1955)
                     /locus_tag="ACHKV2_005197"
     CDS             complement(1686..1955)
                     /locus_tag="ACHKV2_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO6942881.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2025..2609
                     /gene="mobA"
                     /locus_tag="ACHKV2_005198"
     CDS             2025..2609
                     /gene="mobA"
                     /locus_tag="ACHKV2_005198"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO6942882.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2591..3118
                     /gene="mobB"
                     /locus_tag="ACHKV2_005199"
     CDS             2591..3118
                     /gene="mobB"
                     /locus_tag="ACHKV2_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO6942883.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          820 a          765 c          759 g          793 t
ORIGIN      
        1 tttcactgta tcagcatact gctgaacaaa aacatccata ttgctggtat ccataccctg
       61 cggattcagc tgatatttac cgttaacaaa catcgccgga acaccacgca attgcacgtc
      121 agctgcagct ttttcctgct gagcgaccag agatttcacc acgaagctgt tccacgccgc
      181 gtcgtactct tcacctttaa tacctgcgtt gataaataca tcgcggatat cagatgctga
      241 acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt tgtcttccac
      301 gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt caccacccat
      361 gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct tcacattatc
      421 agaaatatgc agaacttctt caaactgata gcagtgcggg cagaagaaag agaaaaactc
      481 cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt taccatcttc
      541 atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca gccaaatctt
      601 tttcatgatc tactctctcc gattaataca taggtgttaa ttgcaaaggg ggttcttgta
      661 gaacttttgc ctgttctata aaagtcgccg tctgtcgtag ccagtaatct tccccggtta
      721 accacgggaa atttttcggg aacgcgggat cagcccaacg ccgcattagc caggcaagat
      781 aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact
      841 cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc tcggctttat
      901 cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca tcatccagat
      961 caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct ccatgtagcc
     1021 gcagaacggt gaaatcttcc cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg
     1081 ctttcaggaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca aacagcttgc
     1141 gtggctcaat gagatattca ttcagaccga tggtcgggcg atggataaaa agctgtttgc
     1201 gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga tcgatattat
     1261 cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat
     1321 tcaataaagt ctgaccgtta aaagccacag gtgctgcaac cggaacttca tcatttacca
     1381 gctgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt tcaggacgat
     1441 aaaatttgac gacaaaacgt cgacgatctt cgtcctgaaa ttgatagaca cggttttcat
     1501 agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaac agagcgtcca
     1561 tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc ccatcatccg
     1621 gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc gccgcttaca
     1681 agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca
     1741 taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat tttcaggtga
     1801 tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga ctctttcgcc
     1861 agtttctgca aaaattgcaa caggttaagg tctggctctt tttgccaggc tggctggagg
     1921 agttcaataa cttcattcag acgtttacat ttcatagtaa tgctccttac tcttgagaca
     1981 gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat ctgatgacga
     2041 cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggcggc gtagataaag
     2101 gattgctgga cttaaatggc aaaccgctgt ggcaacatgt cgctgacgcg cttatgacgc
     2161 agctctctca cgtcgtggtt aatgctaatc gtcatcagga aatctatcag gcaggcggtc
     2221 tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcaggg atgctttcag
     2281 taatgcagca ggaagctggt gagtggtttt tgttttgtcc gtgcgatacg ccttacattc
     2341 cccatgattt agccgcccgg cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc
     2401 atgacggtga acgcgatcac ccgactattg ctatggtaaa ccgcgctatt gagcctttat
     2461 tactagaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt ctggctggcg
     2521 gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat acgccagagg
     2581 agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg cgtggagtgg
     2641 taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca gagggatccg
     2701 tccagggctg attaagcata cgcaccatga tatggatgtt gataagccag gcaaagatag
     2761 ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc aacgatgggc
     2821 cttgatgacg gaaacaccag acgaagaaga gctggatcta cagtttctcg caagtcgaat
     2881 ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag agatcgcaaa
     2941 gattgtgctg tttcgcgatg gagccggaca tcgacctgaa gaattagtga tagacaggca
     3001 tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac tggatattaa
     3061 tgatgttgag gggctggctg attttgtggt ggagtggatg caaaatcagg acggataagc
     3121 aagagtaggc tggagca
//
LOCUS       DAZFTN010000265         3076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFTN010000265 DAZFTN010000000
VERSION     DAZFTN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..240)
                     /locus_tag="ACHKV2_005200"
     CDS             complement(<1..240)
                     /locus_tag="ACHKV2_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO6942884.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGY"
     gene            complement(310..819)
                     /locus_tag="ACHKV2_005201"
     CDS             complement(310..819)
                     /locus_tag="ACHKV2_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO6942885.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTFVLLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHSALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            complement(816..1112)
                     /locus_tag="ACHKV2_005202"
     CDS             complement(816..1112)
                     /locus_tag="ACHKV2_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942886.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            complement(1102..1299)
                     /locus_tag="ACHKV2_005203"
     CDS             complement(1102..1299)
                     /locus_tag="ACHKV2_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942887.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            complement(1292..1624)
                     /locus_tag="ACHKV2_005204"
     CDS             complement(1292..1624)
                     /locus_tag="ACHKV2_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942888.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEEAEKIVSFMDKECDKRDMPEI
                     IMDDMILDSAVALSPLWIVHSLAEIAKGADKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(1640..1990)
                     /locus_tag="ACHKV2_005205"
     CDS             complement(1640..1990)
                     /locus_tag="ACHKV2_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942889.1"
                     /translation="MRSYIDNEKLETISDCLSLLAKIKETIEEIKFQLEYAPCGDDAW
                     RNAARKALAVFQKQRRTVEYHLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            complement(1987..2316)
                     /locus_tag="ACHKV2_005206"
     CDS             complement(1987..2316)
                     /locus_tag="ACHKV2_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942890.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREVNA"
     gene            complement(2313..2864)
                     /locus_tag="ACHKV2_005207"
     CDS             complement(2313..2864)
                     /locus_tag="ACHKV2_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO6942891.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTRISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            complement(2868..>3076)
                     /locus_tag="ACHKV2_005208"
     CDS             complement(2868..>3076)
                     /locus_tag="ACHKV2_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942892.1"
                     /translation="EYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGV
                     KPAKQKMTEILSDEFYAAEKRVRG"
BASE COUNT          767 a          831 c          660 g          818 t
ORIGIN      
        1 ataaccccgt ccgcccatat aggctgcctg agccgcagcc accttgcgtg ccagtttacc
       61 ggccagtacc ggctcaatcc cctgacgctg aagttctgcg ccggtgactt caaccagttc
      121 tgacagggtc cgcggccagt tctttttgag aacatcatca gggatgtcat caaggcggtc
      181 gataagggcg tgcagttgct cactgtcacc accaaacatg ctcatctgag tttctgccat
      241 atcagcctcc ggttctcgcg ttcccggtaa gtttaaaata aaaatcccgc gttgtggcgg
      301 gatttggggt taacggggaa gtgatttttc atactgcctg caaacggcgt cataccccat
      361 cagcgtgcgt tcgggcattc ccatcgcggc cagcattttg cggcggtgcc atcgcttgag
      421 acgctccagc acatctgagg ccagggcgga gtgttgctcc agccactgcc agtttgccac
      481 accttcgccg ccgttctgcg cggccgtctg cgatttcacc cagcggttaa gagcggtttc
      541 cgcaccatca gagataaagc cctgccggtg catcaccttc cagatggcac gaattttggc
      601 ggtcaccgta cccggtttta aggcccggtt aaccggcttc tggcgcactt taaaaccgcg
      661 ttttttgaaa acatccagca cgcgggataa ctcatccggt gacatatccc ggcagctggt
      721 tttgccggta gctgccagca gcacgaatgt ataagtctca tcatccaggg caagctcgcg
      781 ctttgccaca tgaatggcgg atatcagttt tcctctcata ccgtatttac ctttttacgg
      841 acatggaaag ccttttctgc ctcatcccac cgggcatggt gtgttggcgg aataatcatt
      901 atgtgccagc catctctggt tgttattcgg cagaaaaatt caccgctata taacacaatg
      961 gcctgctgaa tgagcgcatc agccgcccgt tcaaccggat gtcttttatc aaaaaagcag
     1021 atgatttttc gacataacca ccgggtaata acaaatgcgc cgaaaaagga tgtgatatat
     1081 aagcaccaga acaacagaga actattgtcc attatctttc tccttattca ctcttctgcg
     1141 gcattcggca cagtcatcag ggctttcaaa tgtatccggc tcgcgattat gagccagata
     1201 taaccaccgt ccacatatac tgatattttc gcctgtttta aaaaaatgat gcttcctggc
     1261 cattagtggc cgcgcccatc cagcgggtaa actattcatt tgctttatcc tccatactgg
     1321 agagaatcat atgtattaat gtcgggcgtg gagatatacg catttcatta agcgtctgaa
     1381 gggcagcaac agctgcctgt ttatcggcgc ctttggcaat ctctgcaaga gaatgcacaa
     1441 tccataacgg acttaacgcc acggctgaat ccagaatcat gtcatccata ataatttctg
     1501 gcatatcccg cttgtcacat tctttatcca taaaagaaac gattttttct gcttcttccg
     1561 gtttcatgcc tccggcgatg agaattttac gatattcata agacgatctt aattcaatac
     1621 tcatcattca cctgcctgtt taatcagttt ccagtgcttt gctacgggca acagcctcac
     1681 actcaaaaaa taccgactcc ggcacacgct cctttaattc acgaacaaga aaatcattaa
     1741 cgagttcatg acgtcttata tttctttcct tttcctcctg gcgcaataca gcaaggtggt
     1801 attcaacagt ccggcgttgt ttctgaaaga cagccagtgc ttttctggca gcattacgcc
     1861 atgcatcatc gccacatggt gcgtattcaa gctgaaattt aatctcttca atagtttctt
     1921 tgatttttgc cagcaagcta agacagtcgc ttattgtctc cagcttttca ttatcaatat
     1981 aacttctcac gcattcacct cccgtaattc cggtaaagcc gaaatatgac accagtgcgt
     2041 cacctgtccg tcttcatctt caaggcaggc tccggtttct tcatcaacaa aaaagccttc
     2101 ataccccata tggccgatgc gcagctcctg acggtgacca tactgaacaa tcagcacgac
     2161 agcctccata tcaggtggga aagcatcatt cagtgaatgc cagggataac cgcgtaaatc
     2221 cccgaaataa acaccggtca gcgttccgcc acagagcggc attccggcgg cacacacaag
     2281 aggttcatct gattcaacaa ataaggtcgt tatcatggcg ttaactcccg taatacagcg
     2341 tcctcggcag tccggcgacg gtaataaact gcgtgagcag aatgttcaga tattccaaac
     2401 ttttcgccaa tttcccggaa tgacagacgg cgtgggtaat cggaatcacg cagcgcacgt
     2461 ataagacgta catcatcatc agatatgcgc gtgccggggc gtaactctcc gcactttgtt
     2521 aatccagcac caatcattcc tgcccgacat ctgacagaat ctttattacg tccgagatat
     2581 tctcccattt gctggcaggt cattgttctg gcattttttc gaataaactt atcttcatca
     2641 ggtgtaaatc ttgggtgttt atatttcagt aactccgggt atgaactgcg caatacacca
     2701 gtccggttat atacagccca gacagttctt tttattctgg cagcaatatc cttaaccggg
     2761 gtggatgaat aaagcgcaat cagcagcgca tcttcttcag gtgtccacgg acggacatga
     2821 gcgggggcgc gaccttttag tcccatgggt tgtaatttca tcatgcatca ccccctgact
     2881 cgcttttcag cagcataaaa ctcatcagac agtatttcgg tcattttctg ttttgcgggt
     2941 tttacgccgc actcaaggta aatcacatcg tcaacgcaga accacttcac agggccaaac
     3001 atgattgccg agaaatcaat acccagccag aacaacagtg catcggttct ggcatacgtg
     3061 acgggcgaat attcac
//
LOCUS       DAZFTN010000266         3055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFTN010000266 DAZFTN010000000
VERSION     DAZFTN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..781)
                     /locus_tag="ACHKV2_005209"
     CDS             complement(<1..781)
                     /locus_tag="ACHKV2_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO6942893.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLRQYGDFENAIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAPPELLN
                     ILDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCEVPDELIDFTFEWKGLKKL"
     gene            complement(1527..2090)
                     /locus_tag="ACHKV2_005210"
     CDS             complement(1527..2090)
                     /locus_tag="ACHKV2_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO6942894.1"
                     /translation="MDNSKHSDEAEKLLAELSARKGEGEGEGEGEGEGEGEGEGEGEG
                     EPKSTVSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANN
                     GHATAQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEAL
                     QWAYKLKASKQPLDADRLIEELTKEQK"
     gene            complement(2159..>3055)
                     /locus_tag="ACHKV2_005211"
     CDS             complement(2159..>3055)
                     /locus_tag="ACHKV2_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6942895.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          832 a          718 c          528 g          977 t
ORIGIN      
        1 ataatttctt cagccctttc cattcaaacg tgaaatcaat aagttcatca gggacttcgc
       61 aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactaatt gcgtaactgt
      121 catgctctgg attatttaat tctttcagcg gaaatttttc ctcaaaggct ttatttagcc
      181 gcccctggtt tccttttacc gcgaataaat aatcacctcc ctgtttttgt atcttctctg
      241 caatatcttt ctggcacccc atcgcatcag ttgtgatgat ttttccttta atatccagta
      301 tgttaagaag ttcaggggga gctgtaatct cattagattt ctcatccgtc ttgatctgtc
      361 cgatgaccag actgtgcatt gttgagaacg cactaatgac atgaatcgct cccctgcggc
      421 gactcttgtc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg tctttatcat
      481 ctgaagaatg gcagtcacgc atccagttaa taaagcactc atgaaatttt gcaggactga
      541 tacaggatac aactctggca atggtatcgt ggacaggaat agcattttca aaatcaccat
      601 attgcctcaa aaaatcgaga tgcgtttcac caaaatcctc tatatcttcc caaccttctg
      661 caccagaaat gacggcacaa atagtcaaca gtagaatatc tgacaattta tgttctactt
      721 tccaggcttg tctgtaatcg gggataatag aaatatgttc catcaatttt ttaagttcca
      781 ttttgttctc cttatttatg caaggagtat ttgatcatat gtaagctgtg aaaaacagtt
      841 ttagataata aggaacatct caattattga acatttagtg cgaattattt agtacaaaaa
      901 agcggcgtta ggtgatcttt ccctgaggtg aggagatatt aacatggaca aaaacaagaa
      961 acttatgaca gaagaaatat aaggaattat tatacaagct attaaatcaa caataaaaga
     1021 tcttttaaaa aataacaaaa aacagaacac tatcaaaaca taaaaagcaa acaaaaaaca
     1081 ataagcatgt tgattgaata ttttaaatac atgttctttt acaataaaaa aatgaatggt
     1141 tatcgaacaa gccagcaata ataaccttat tattttcata agctccatcc actaaaatgc
     1201 tggagatata aaaaatgaac caaacggctt ctttggccca tacttaggtt tggggtgtag
     1261 aatcttagtc ataagatcta tgccgagtca tgtgctggcc acatacttaa tgatacaaaa
     1321 agctatattt tcatcttttc tcttgcgact tttgatgcat ctgccaagta catcgtaatg
     1381 ataaccggcg acgaagatgc cagttttggt ttcaacttgc gctttgatga gcttgttgca
     1441 gtcgtcgtaa acgtaatttt gtgtgtaggt gttgtagcga ctggtaaagt ttttctactc
     1501 tccatttttc tgttacctgt ttaaagctac ttttgttctt tcgttaattc ttcaattagt
     1561 cgatctgcat caagcggctg tttactcgct ttgagtttat atgcccattg caatgcttct
     1621 tttttatttc cctggttttt ataacaattc accaagaacc atattgctga actgtcatta
     1681 ttactggcag ccaatttaat tttttgtaac cattcattag cttccttggt ttcaccttgt
     1741 tcttgtaact ctacatataa ccgatactga gctgtcgcat gaccattatt agccgctatt
     1801 tttaaccact gactactttt ttcttcatca taattggaca acttgtagta caagaacaac
     1861 ctaaaacccg cctcgccatc gcctcgtttt gcctggtgtg ataaagtatt tacttcttct
     1921 ggctgcaaat aaaagacgct tactgttgat tttggttcac cttcaccttc accttcacct
     1981 tcaccttcac cttcaccttc accttcacct tcaccttcac cttcaccttt tctagcagaa
     2041 agctccgcca ataatttttc cgcctcatca gaatgttttg agttatccat agcagccctc
     2101 actccaaagt tataaagaca taccatgata atcaacaaaa aagtaaatac tttcattact
     2161 atttgcttcc tgtaggtgta aacttatgaa agtcccctgg ttgtttctcg tctggcacca
     2221 attcaaacac cccgttgtcc ttaccgccta ttgttatcct agctgtttgc cccataatag
     2281 catagtttcc atccggccaa atccatatta tttcatcagg agcaatcact gtagcatcaa
     2341 gttcgttagc tagtttctgt gcaaatgaat ttttgccttt tcctgtttcg caggaaaaca
     2401 agactacagg cataccttca tgatatttag gattcgctct tatgtcgcta gctaattgtt
     2461 ttattgtcag atcacttcct tttttgattc tgtcatcact catagatgta ggcgaaccat
     2521 gacctccaac tgtaaaaccc gttggaaatt cccttacatt aagagaacct ttatatatag
     2581 gatctgacgg agaataataa ttcaaatcaa gtgctaaccc caacggatct atatgttcaa
     2641 taggattgag tgggtactga tacaggttcc atcccccctc cagccctatc gggtcctggg
     2701 tgatatatct cccctgcaac ggatcatagt accggttgcg gttgtagtac agccccgact
     2761 cctcatcata ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc gcgctggttt
     2821 cgcccagcag gtttccccac tcgtcatact ccccctgcca cgccgtttcc ccttccgggc
     2881 tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgaagt ttcctctccg
     2941 ggatgtattc cgcctccagc tgcgcggcca tctgctccgc cgtcagcccg cactgcgcaa
     3001 gccactgctg gctttcttca ctcacgctgc cctgccgcag ctcccgctcc agcct
//
LOCUS       DAZFTN010000267         2998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFTN010000267 DAZFTN010000000
VERSION     DAZFTN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..153
                     /locus_tag="ACHKV2_005212"
     CDS             <1..153
                     /locus_tag="ACHKV2_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6942896.1"
                     /translation="KSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDNKDI
                     ICLMRQ"
     gene            135..1583
                     /locus_tag="ACHKV2_005213"
     CDS             135..1583
                     /locus_tag="ACHKV2_005213"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO6942897.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIV
                     QTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENP
                     GIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            1587..2957
                     /gene="cpsG"
                     /locus_tag="ACHKV2_005214"
                     /gene_synonym="manB"
     CDS             1587..2957
                     /gene="cpsG"
                     /locus_tag="ACHKV2_005214"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6942898.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
BASE COUNT          866 a          619 c          720 g          793 t
ORIGIN      
        1 aagtcacaac ataatgctta ttgctggcta tcgcgagcaa agctgataaa tgatgacgat
       61 gtgcattata attgtcgcgc atattttaac aataaaacaa atgatgcgat tggcttagat
      121 aataaggata taatatgtct gatgcgccaa taattgctgt agttatggcc ggtggtacag
      181 gcagtcgtct ttggccactt tctcgtgaac tatatccaaa gcagttttta caactctctg
      241 gtgataacac cttgttacaa acgactttgc tacgactttc aggcctatca tgtcaaaaac
      301 cattagtgat aacaaatgaa cagcatcgct ttgttgtggc tgaacagtta agggaaataa
      361 ataaattaaa tggtaatatt attctagaac catgcgggcg aaatactgca ccagcaatag
      421 cgatatctgc gtttcatgcg ttaaaacgta atcctcagga agatccattg cttctagttc
      481 ttgcggcaga ccacgttata gctaaagaaa gtgttttctg tgatgctatt aaaaatgcaa
      541 ctcccatcgc taatcaaggt aaaattgtaa cgtttggaat tataccagaa tatgctgaaa
      601 ctggttatgg gtatattgag agaggtgaac tatctgtacc gcttcaaggg catgaaaata
      661 ctggttttta ttatgtaaat aagtttgtcg aaaagcctaa tcgtgaaacc gcagaattgt
      721 atatgacttc tggtaatcac tattggaata gtggaatatt catgtttaag gcatctgttt
      781 atcttgagga attgagaaaa tttagacctg acatttacaa tgtttgtgaa caggttgcct
      841 catcctcata cattgatcta gattttattc gattatcaaa agaacaattt caagattgtc
      901 ctgctgaatc tattgatttt gctgtaatgg aaaaaacaga aaaatgtgtt gtatgccctg
      961 ttgatattgg ttggagtgac gttggatctt ggcaatcgtt atgggacatt agtctaaaat
     1021 cgaaaacagg agatgtatgt aaaggtgata tattaaccta tgatactaag aataattata
     1081 tctactctga gtcagcgttg gtagccgcca ttggaattga agatatggtt atcgtgcaaa
     1141 ctaaagatgc cgttcttgtg tctaaaaaga gtgatgtaca gcatgtaaaa aaaatagtcg
     1201 aaatgcttaa attgcagcaa cgtacagagt atattagtca tcgtgaagtt ttccgaccat
     1261 ggggaaaatt tgattcgatt gaccaaggtg agcgatacaa agtcaagaaa attattgtga
     1321 aacctggtga ggggctttct ttaaggatgc atcaccatcg ttctgaacat tggatcgtgc
     1381 tttctggtac agcaaaagta acccttggcg ataaaactaa actagtcacc gcaaatgaat
     1441 cgatatacat tccccttggc gcagcgtata gtcttgagaa tccgggcata atccctctta
     1501 atcttattga agtcagttca ggggattatt tgggagagga tgatattata agacagaaag
     1561 aacgttacaa acatgaagat taacatatga aatctttaac ctgctttaaa gcctatgata
     1621 ttcgcgggaa attaggcgaa gaactgaatg aagatattgc ctggcgcatt gggcgtgcct
     1681 atggcgaatt tctcaaaccg aaaaccattg ttttaggcgg tgatgtccgc ctcaccagcg
     1741 aagcgttaaa actggcgctt gcgaaaggtt tacaggatgc gggcgtcgat gtgctggata
     1801 tcggtatgtc cggcaccgaa gagatctatt tcgccacgtt ccatctcgga gtggatggcg
     1861 gcatcgaagt taccgccagc cataacccga tggattacaa cggcatgaag ctggtgcgcg
     1921 aaggggctcg cccgatcagc ggtgataccg gactgcgcga tgtccagcgt ctggcagaag
     1981 ccaatgactt ccctcctgtc gatgaaacca aacgtggtcg ctatcagcaa atcaatctgc
     2041 gtgacgctta cgttgatcac ctgttcggtt atatcaacgt caaaaacctc acgccgctca
     2101 agctggtgat caactccggg aacggcgcag cgggtccggt ggtggacgcc attgaagccc
     2161 gatttaaagc cctcggcgca ccggtggaat taatcaaagt acacaacacg ccggacggca
     2221 atttccccaa cggtattcct aacccgctgc tgccggaatg ccgcgacgac acccgtaatg
     2281 cggtcatcaa acacggcgcg gatatgggca ttgcctttga tggcgatttt gaccgctgtt
     2341 tcctgtttga cgaaaaaggg cagtttatcg agggctacta cattgtcggc ctgctggcag
     2401 aagcgttcct cgaaaaaaat cccggcgcga agatcatcca cgatccacgt ctctcctgga
     2461 acaccgttga tgtggtgact gccgcaggcg gcaccccggt aatgtcgaaa accggacacg
     2521 cctttattaa agaacgtatg cgcaaggaag acgccatcta cggtggcgaa atgagcgctc
     2581 accattactt ccgtgatttc gcttactgcg acagcggcat gatcccgtgg ctgctggtcg
     2641 ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaaat ggtgcgcgac cggatggcgg
     2701 cgtttccggc aagcggtgag atcaacagca aactggcgca acccgttgag gcaattaatc
     2761 gcgtggaaca gcattttagc cgcgaggcgc tggcggtgga tcgcaccgat ggcatcagca
     2821 tgacctttgc cgactggcgc tttaacctgc gctcctccaa caccgaaccg gtggtgcggt
     2881 tgaatgtgga atcacgcggt gatgtaaagc taatggaaaa gaaaactaaa gctcttctta
     2941 aattgctaag tgagtgatta tttacattaa tcattaagcg tatttaagat tatattaa
//
LOCUS       DAZFTN010000268         2975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFTN010000268 DAZFTN010000000
VERSION     DAZFTN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..253)
                     /locus_tag="ACHKV2_005215"
     CDS             complement(<1..253)
                     /locus_tag="ACHKV2_005215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942899.1"
                     /translation="MFEKYRHDFPRKIFINSENPRGRRPVTGRIAGKDPQNDNNYHLH
                     VTTCIYAIKPRQIINYDAGIVLIDLHQINVKATSDNTNQQ"
     gene            367..912
                     /locus_tag="ACHKV2_005216"
     CDS             367..912
                     /locus_tag="ACHKV2_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6942900.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            887..2812
                     /locus_tag="ACHKV2_005217"
     CDS             887..2812
                     /locus_tag="ACHKV2_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6942901.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLAEYARALSGEDE"
     gene            2809..>2975
                     /locus_tag="ACHKV2_005218"
     CDS             2809..>2975
                     /locus_tag="ACHKV2_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO6942902.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT          742 a          701 c          848 g          684 t
ORIGIN      
        1 gttgctgatt tgtattatct gaagttgctt ttacgttaat ttgatgcaga tcaattaata
       61 cgatacctgc gtcataattg attatttgac gtggtttgat ggcgtagatg cacgttgtga
      121 catgtagatg ataattatta tcattttgcg ggtcctttcc ggcgatccga ccggttacgg
      181 ggcggcgacc tcgcgggttt tcgctattta tgaaaatttt ccggggaaaa tcatgtcggt
      241 acttctcgaa cataactatt tgttttttct aatatcgaat ccgtaaaggt ccgacatgaa
      301 aacgcctgaa aaagtcattt tcgggcactt tcatgtcgga ccctgtattt gttgtgagac
      361 tgtttcatga aggttaataa aaagaaactt gccgaaattt tcaacgtgga tccgcgaacg
      421 attgaacgct ggcagtctca ggggctccct tgcgtctccg gaggtggtaa gggcgttgaa
      481 tctgtatttg ataccgccac ggcaattcag tggtatgcgc agagggaagc tgatatcgaa
      541 aatgaaaaac tccgtaaaga ggttgaggat tacagggctg ccagcgaggc agatctccag
      601 cctgggacta ttgagtacga acgccatcga cttacgcgtg cgcaggctga cgcccaggag
      661 ctgaagaatg ccagagactc cgcagaagtg gtggaaaccg cattctgtac tttcgtgctg
      721 tcacggatcg caggtgaaat tgccagtatt cttgacggga tccctctctc agtacagcgg
      781 cgttttccgg aactggaaaa ccgacatgtt gatttcctga aacgggatat tatcaaagcc
      841 atgaacaaag cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa
      901 cagtcagatt gatattctgc ggcgtgatgt acgcgccggg ctgcgagccc tgttcaggcc
      961 ggagccacag actgccgttg aatgggcgga tgccagttac tatctcccga aagaatccgc
     1021 ataccaggaa gggcgctggg aaacactacc ctttcagcgg gctatcatga atgcgatggg
     1081 cagcgactac atccgcgagg tgaatgtggt gaagtctgcc cgtgttggtt attcaaaaat
     1141 gctgctgggt gtttatgcct acttcataga gcataagcag cgtaacaccc ttatctggtt
     1201 gccgacggat ggtgatgccg agaactttat gaaaacccac gtcgagccta ccatccgcga
     1261 tattccgtcg ctgctgtctc tggccccgtg gtatggcaaa aagcaccggg ataacacgct
     1321 cactatgaag cgtttcacca atggtcgtgg cttctggtgc ctgggcggta aagcggcaaa
     1381 aaactaccgt gaaaagtcgg ttgatgtggc gggttatgat gaacttgctg cctttgatga
     1441 ggatattgaa caggaaggct ctccgacgtt cctgggtgac aagcgtattg aaggctcggt
     1501 ctggccaaag tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg
     1561 tgcagccagt gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga
     1621 ggagcagtat cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga
     1681 tgacccctcc agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga
     1741 gctggacttt actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg
     1801 cattctctgg ttttcgtcat ccggtgaaga gattgaaccg cctgacagtg tgacctttca
     1861 catctggacg gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa
     1921 gacgaaaggg gatacgggaa aacgtaaaac cttcgtgaac accacgctcg gtgagacgtg
     1981 ggaagcgaaa atcggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta
     2041 ttcagcgtcc gttcctgatc gtgtggctta cctgaccgcc ggtatcgact cccagctgga
     2101 ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgaccg
     2161 gcagattatt atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat
     2221 caataaaacc tatactcgcc ggaatggtgc agaaatgtcg gtatcccgta tctgctggga
     2281 tactggcggg attgacccga ccattgtgta tgaacgctcg aaaaaacatg ggctgttccg
     2341 ggtgatcccc attaaagggg catccgtcta cggaaagccg gtggccagca tgccacgtaa
     2401 gcgaaacaaa aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat
     2461 ttataaccgc ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt
     2521 cccgaataac ccggatattt ttgatcttac cgaagcgcag caactgactg ctgaagagca
     2581 ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa
     2641 tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg
     2701 gcagctggat ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa
     2761 caagaaaaca ctggcagaat acgcccgtgc cttatccgga gaggatgaat gacgcgacag
     2821 gaagaacttg ccgctgcccg tgcggcactg catgacctga tgacaggaaa acgggtggca
     2881 acggtacaga aagacggacg gcgagtggag tttacgacca cttccgtgtc tgacctgaaa
     2941 aaatacattg ctgagctgga agtgcagacc ggcat
//
LOCUS       DAZFTN010000269         2918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFTN010000269 DAZFTN010000000
VERSION     DAZFTN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2539)
                     /locus_tag="ACHKV2_005219"
     CDS             complement(<1..2539)
                     /locus_tag="ACHKV2_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFO6942903.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNA"
     gene            2751..>2918
                     /locus_tag="ACHKV2_005220"
     CDS             2751..>2918
                     /locus_tag="ACHKV2_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942904.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGAL"
BASE COUNT          899 a          627 c          529 g          863 t
ORIGIN      
        1 gagcatttag taataaatca tttgtctgca atacaggtag catccattta attactggtg
       61 caatgcgagc tgtaccatca agttttccca caattaacca tggactatcc tgaacattca
      121 gagctgaaag atcataagca aactcatctt tttttaacaa tgatatgtgt aagccaggat
      181 tcttaagaga aatagcatca acaaccagtt cgtctattct atttgcagga aaaacaacag
      241 aattttttat atcaaccagt aacccttgat cgtgttcaat aaaactacca tcgtagcgtt
      301 taatattgag tgttgcttta tcctttccaa atctgtcaga gtaaaaatca acacataatg
      361 tactattgct gttccatgca catgccagca tagcatttag caaattatag ttctcataaa
      421 gtgaaatttg ttgtaatttg tcattgcgtc ctgataaaag aggaaggtcg gcatgtaaat
      481 agcggaattt ttcagctact ggattttcat ccaataactc gatattaaga tatcctcttc
      541 cagcacatga tatggttaat aactcagcat ctattccatg caaatgtgct atagatgcca
      601 caccatcaaa atgaacagaa ccatcattag ttacaaatcg gccaccgcta accggcttag
      661 gtaataatag tttaagatta gaaccatctt tccaggagac ccgtaaatca acggtatttt
      721 ccagcatcag cgatgaatta taataaatag cacactcgtt ttgtgaataa tcggacttaa
      781 gtgttacttt ttcattgttt gaatatttat caattctagt atccgttata ccagaaagca
      841 taataatgcc ttgctgagca ctctcaggta taatattaat atcgaagtct gctggtaaaa
      901 ctacaacttt accgctaaat aaaacctcat cattaactat tcgccggact tctatctgtc
      961 cttttggcat ttctgatgcg acagaatacc aggcattatt acctgaacga atggaacgcc
     1021 aaaaaagttc tttctccggt acaatgccat attgcagatc ctttttccaa ccgatcttcg
     1081 gccaggctcg atggacagga taatccgatt taaccagttc aacttctgtc gatttaatat
     1141 aatattcaat cgcagaatca taaagttgct gggtgcgaat tgtacaaaca gcaccatcat
     1201 gtaaaacaac gctaaatact ccacttattt tagttaagct gcgttcactg tttttcagca
     1261 accttgggat atcaaactca ccttctccac taatatctaa atggctattt ttcggtagcg
     1321 aaataaacag agcatttagc ctggaactca cagaccccat accaacaagt tttagctgcg
     1381 attcactctc attcatcgct tcaaaaaccc agggaagttc ttcagtaagt tcataaccgc
     1441 cttttggaat tgtgtgacct aacagaattg gaccttcatg aagcgacaat gagatttcgg
     1501 ccattgcatc tgcaccagag agtttttgca tcgcaatagg taataactcg acccgccaat
     1561 cttgctgctc ataacggctt agcattgcca accttgcccc gccatttttc cattttcctg
     1621 aaatgatgag tcgggtctgc tccggctgaa tatggcattc aaacaaagag gttaactgtt
     1681 ctgtacgcat tgtggccggg aatcggaacc gggcatcgca ataccaactg tcatcaacat
     1741 cgacccagat tctttcaacc tgaagactac ttgcactgcg tatttcagaa gattgagaaa
     1801 gtaatcgcct tacaatctct gcagcattcg cttcggggag aacgagtgga agttgaagat
     1861 gccagtctgg agagagctta cgtaatgcag aaacgggatc actgctatga ggaggatgct
     1921 cactaagtaa tcggcagaga gtctcgcaga attcaccggc tatttcatag accagttcgt
     1981 tttgcattgt gacaggaatc gtatcgccta attcttgtgc aattttagcg gcaggatatt
     2041 gagatgattg cccacgaagc gcctgatata tccttctgaa ataggtaatc aaataaccgc
     2101 tctcattttc aatcatgcgg ataggcaaac caccttcaca agccagtgta tgcagataac
     2161 ccgaagcttg tcccaggttt cttatctttc ttttccagta gcgaatgcca tcattaacca
     2221 gcttctgtcg atgaacatac ggaactttcc agttaataga atctaaaatt gtgtcccatt
     2281 tagggtgtcc gaccgtatga gtccggcgga taaactctgc ggcatatatg ctaaatagaa
     2341 catcgctata ggaaatgaat atcgttcttg gataactacg gggggcatat gtacgcagta
     2401 gttctgccaa actttcgtac tctgtatcag tacaacggta agcatataac gcccgtccat
     2461 cagctccgga taatgaacgg cgactaaaaa acatgttaag ccattgcaaa agtgggttaa
     2521 ctggagtttt cacaagcaac ctgccttcct ttgatacaat tcgtaacagg ttactatcat
     2581 cataaaaaag ctcaacccga tgaactcgct aaaaatgaga caaatcattt atatctcgaa
     2641 aaaacttgtt acaatcatga gcgctacacc gaacttaacc atataaatta tgtgtgtttt
     2701 gtttattttt taaacgatta caactatcca ttatttacac aggtatcaaa atgttagcgc
     2761 agctttttga gcagttgttt caatcgatag actctacact gatcaccaat attttcatct
     2821 gggctgttat attcgtattt ttatcagcgt ggtggtgtga caaaaaaaat atacatagta
     2881 agtttagaga atatgctcca accttaatgg gggcatta
//
LOCUS       DAZFTN010000270         2881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFTN010000270 DAZFTN010000000
VERSION     DAZFTN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..1951
                     /locus_tag="ACHKV2_005221"
     CDS             14..1951
                     /locus_tag="ACHKV2_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6942905.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVPGTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTY
                     DRPDPRIKQLARRSTVTPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLAD
                     MVGQCAGGSADGVPWICGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     ANVPTNKPEEDPDIPASGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILL
                     HAGRTAELVGGEQVVMPPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWG
                     AEGGRALVVDDAGAAGGKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDG
                     TLTANQYALALYWPVSSLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNT
                     KLGSFGAFDNEWHTLGFRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTD
                     ITSGATYPVLIESITVEVNNP"
     gene            2099..2281
                     /locus_tag="ACHKV2_005222"
     CDS             2099..2281
                     /locus_tag="ACHKV2_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6942906.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2319..2588
                     /locus_tag="ACHKV2_005223"
     CDS             2319..2588
                     /locus_tag="ACHKV2_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6942907.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2664..2879
                     /locus_tag="ACHKV2_005224"
     CDS             2664..2879
                     /locus_tag="ACHKV2_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6942908.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          716 a          672 c          868 g          625 t
ORIGIN      
        1 cgggagaaat tcgatgtcga ttaaacatta tgatgttgtc agggcggcgt cgccgtcaga
       61 tcttgcggaa aagctgacac acaaactgaa agagggctgg cagccgtttg gtagtcctgt
      121 ggccataacc ccttataccc tgatgcaggc gattgccgcg gagggggatg tgaccacgcc
      181 agtggttgtg cccggcacgg gggatggtgg ctatccggga gtggtcacca cggagccaga
      241 ttattactac gttattccac tggccgggca gtcgaacggc atggcttacg gtgaggggct
      301 tcctctgccg cagacatatg accgtcctga cccgcgtata aagcaactgg ctcgtcgcag
      361 cacagtgacg ccggatggcg ctccctgtaa atataacgac attattccgg cagaccactg
      421 tctgcatgat gtacaggaca tgagccgtct taaccatccg aaagctgacc tgtcgaaagg
      481 tcagtacgga accgtggggc aggggctgca tattgccaaa aagctgctgc cgtttatacc
      541 ggcgaatgcg ggtattcttc tggttccgtg ctgccgtggt ggttcagctt tcaccaccgg
      601 ggcagatgga acatacagtg acgtgaccgg tgcctcagag agttctaccc gctggggtgt
      661 gggcaggccg ctgtataagg atctcatcgg tcgtacaaaa gccgcgctgg caaagaaccc
      721 gaaaaatgtg ctgcttgccg tggtgtggat gcagggggaa tttgactttg acggaacgcc
      781 agcaaatcac acagcccgtt ttacagaagt agtggaacaa tatcgtacgg accttgcaga
      841 tatggtggga cagtgcgctg gtggttctgc tgacggtgtt ccctggatat gtggagacac
      901 aacttatttc tggaagcaga agagcgaatc cacttaccag acggtgtacg gcagttacaa
      961 aaacaaaacg gaaaagaata ttcactttgt gccgttcatg accgatgaga acggagcaaa
     1021 tgtcccgacg aacaaaccgg aagaagaccc ggatattccg gcatcaggat attacggtgc
     1081 ggcctcccgg acgtcggcaa actggacgtc agcagaccgt gcgagccatt tcagctcatg
     1141 ggcacgcagg gggattattt ctgaccgtct tgcctcagcg attcttctcc atgcaggacg
     1201 gacggctgaa ctggtgggtg gggaacaggt tgtgatgccg ccggatgaga agccgtcacc
     1261 ggacacacca tcaacaccgt caacggacgg gaaatcagtg acaacgctgc tttattaccg
     1321 tgcaacagag tcaggtggtt tactgaatcc gcagggatgg ggagctgaag gagggcgtgc
     1381 attggtagtt gatgatgcag gtgctgcagg aggtaaggcg ctgaggtgga ccaaacagac
     1441 aggaagttcc tcgtggttta tgcagcatga tgccggtaat ggcgcagacc tgctggagaa
     1501 gggcgggctt atcagttgtc gttttaaagt tgatggcaca ctgacagcta atcagtacgc
     1561 actggcgctg tactggccgg tttcttcact gcctcagggc gtcacactgg aaggtaatgc
     1621 cggtcataac ctgctggcgt cgttttacgt acagagcgat gccacagacc ttaatgtgat
     1681 gtaccacaag ggaaatgctg gtcagaacac gaagctgggg tcattcggcg catttgataa
     1741 cgaatggcat acgctgggct tccgttttgc cggtaacaac agtattgagg tgacgccggt
     1801 cattgatggt aaggacggga cgccgttcat gctgtcacag tcaccggtcg gcacgtttac
     1861 ggcagacaaa ttgcgcgtga ccgatatcac tagcggtgcg acatatccgg tgctgattga
     1921 aagtataaca gtggaagtga ataacccgta agcaggaaaa aaaggccgcc ggggcaggga
     1981 aaacaaggag ccagaaccgg cggcaaatgt cgttatatcc aaagcaaaac atgcaggaca
     2041 cttttttaac caacaggtat taacgatgtc aacaccatat caataaccgg gagggataat
     2101 gagatttgta cagcttattt tattgtattt ctgcacggtg gtgtgcacgt tatatctggt
     2161 aagtggcggg tataaggtta tccggaacta tatacgcaaa aagattgatg ccgcggcggc
     2221 ggaaaaaatc agcgccagcc agtcagccgg aacaaaaccc gaagagcctc tcatttcgta
     2281 gcaactttct taacaacacc tttcaacgag aaaatcccat gtcagaaata aaatctctgg
     2341 tcactgctga agcagtgaag gacgtcctgc gctctgaaga agtcagaagc gcactgaaac
     2401 agcaactccg ccagaatctt gaggcgcgtc ttgatgctga agtggatgcc attctggatg
     2461 agctgctggg gggaccggct gctcctgagc ctgaagacgg cgcgggtgac agtgctgttt
     2521 cagatggcgt tgtgtctcag cctgacggta gcagtgagcc tcagcctggc ggcgaaatga
     2581 tgatgtaacc atacgcaggg gctgtcggtg tgagctgatg cccctcctgt tgttgtgagc
     2641 ttccggattg cgggagacgg ggtatgtacc agatggaaaa aatcacaacg ggtgtgtcat
     2701 acaccacgtc agcggtgggg acgggatact ggttactgca gctgctggac aaagtctctc
     2761 cgtcccagtg ggtggcgata ggtgtgctgg ggagtctgct gtttggcctg ctgacgtatc
     2821 tgactaacct gtatttcaaa atcagagagg accgtcgtaa ggcggcgcgg ggagagtaaa
     2881 g
//
LOCUS       DAZFTN010000271         2872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFTN010000271 DAZFTN010000000
VERSION     DAZFTN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(437..613)
                     /locus_tag="ACHKV2_005225"
     CDS             complement(437..613)
                     /locus_tag="ACHKV2_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942909.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            788..991
                     /locus_tag="ACHKV2_005226"
     CDS             788..991
                     /locus_tag="ACHKV2_005226"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO6942910.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(923..1396)
                     /locus_tag="ACHKV2_005227"
     CDS             complement(923..1396)
                     /locus_tag="ACHKV2_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942911.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1316..2056
                     /locus_tag="ACHKV2_005228"
     CDS             1316..2056
                     /locus_tag="ACHKV2_005228"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO6942912.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2290..2853
                     /locus_tag="ACHKV2_005229"
                     /pseudo
     CDS             2290..2853
                     /locus_tag="ACHKV2_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          783 a          703 c          664 g          722 t
ORIGIN      
        1 atttgaacac actgacaaaa aaccaagcat ggtaataaac cgaactcccc acaaacttct
       61 gttgcctcag atactccccc ccctttcctc aggcacaaat gttgttccat aacgaaccag
      121 ccctgttcct ttaaactctg catcaatggc ataatgtata ctcaccagac tgagtttgct
      181 cctgcatcat tatttcgata cgttaatgca ttgttcattg catccggcat tccccatcga
      241 atatttactt tgttatatca gtcagcccat aaccaggtaa atttttaagg cttactgact
      301 gaatattcaa aatgtgttac atgttatcaa acctttaaag aataacctgc tcttcttcat
      361 tggccggatt tagctcaatc ttgtcagcaa aactaatata aagccgttca taaagagaag
      421 gatgtagctt cgttatttat cttttacatg taacagtgcc aggggatatt ccgcacatct
      481 ctgtggctat catccccacc acgtctccag tggcagcacc tgcggcactg gcacgggcgt
      541 tatttccctg taccaccgcc agtgcagcat tcaccacggc atgagctgcc acatttgcag
      601 tcttattcac cacacctgta acctgtctct tatacacaaa tccccacccg gggatttgtg
      661 atgtctgtca gtctgcttta gggggactct ttccatccgg tagtttcctt cctccccggc
      721 agcgattttt tgaacttttt cttcacccgt tgatcttctc ttatatttca ttttcttcgg
      781 gtctgttatg gtactttctg attgttattc actggctaac gagcagtctg gacatgcccg
      841 tctcggtgat ccgcgtcgta cacgacgcct tgtgtcactg acctcttctc tcgctcagca
      901 tgcaggtctt tctattgtga aatcatccca cttcactgct caggttgaag gtgcgtaccg
      961 cctgatacgt aacccgtcgg tgtccccgta agccattgcc gaagccggat ttactgccac
     1021 tgcaagggca tgtgaggcac atccgctgct cctcgcgctg gaagatacca ccaccattaa
     1081 cttcggtcac agtacggcat ttgatgacct ggggaatacc accactaacc cgaagacgca
     1141 cgggctgctg gctcactctg tcctgatgta cgcccccgac agcgccctgc cagtcgatct
     1201 tatcgaacag cagtgctgga gtcgtgccac ggacacctac ggtgttaagc accagcgaaa
     1261 agaacgtccc tgtgaagaaa aagaaagtta ccgctggcag caggcttcgg agcgcatggc
     1321 cgaacggctg agggagatac agaaacaggt aatcactgtc tgtgaccggg aagcggatat
     1381 ctggcattac ctgcattata aagtcagccg cgggaagcca cacgctgaat gtgatgcaaa
     1441 aaggcgggcg ggcagcccgc caggcccgga tgctcatcag ttacagcgaa gtcagcataa
     1501 aaaatcccga caacagcggc caggcgctct cgctcacgta tgtctgttgc ggggagcagg
     1561 agaggacgga gcctgctggt atccgctgac atcagaaaaa gtggcgagtg ccgccgatgc
     1621 gcgacgtatc gtcagccatt acgagcaacg ctggctgata gaggaatacc acaaagcgtg
     1681 gaaaagtggt ggtacatgtg tggaatcgct gcgaatgcag acccgggata accttgagcg
     1741 catggtggtt atcgcggtgc gggtgctggg cctgcggcag ggaggtatca gtgaagaaac
     1801 acaaaatgac agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa
     1861 gctggaagga aagcagcttc cggcgcaaac accgaccctg aaatgggcct gtttgaaact
     1921 ggctaaactg gggcgatggc atgacagcaa acgcacaagc agccccggtt gggtagttat
     1981 gtgggatggc tggttcagac atcaggatat ggctgagggt tacctggtga tgaagtctct
     2041 tgatcaggag atctgatcaa gagacaggtc aaagatgggg gacaacgtct ggtgctgtat
     2101 aacagcacag gcctcacact cttgacgccc gtggaatata ttagagaaca aaaatttcaa
     2161 tatgtggtgg acgcatacag aggattgaca gcattcccat actaattgtt aagattcaaa
     2221 ttgaagtata tctatctgtc attttgcaaa tgatatgcgg tagctttacc taactatcat
     2281 gaacatttcg aaaaatcatc cacaaagccc cgaagaataa agcactatcg cccgataaag
     2341 acctgtaagc tcagtggcat ggatactatt gagctcagaa tggcgagctg taccgttatt
     2401 tcattaccat gctcgacgaa cacagcaact actctctggc cgtactatcg cttaaaagtg
     2461 atatcatcag gcatttcttc tgctgtgtag cccagttgtt ccttgttggt atcaaccaga
     2521 tcatcactga tgatgggcaa agagttctcg ggaaaattcg acaaaacgct acaggaagcc
     2581 accgtcgggt tcgtatggac ttatctctat acgccgaaaa taaatgccat aagtgagcat
     2641 tttaaccgaa cactgaggga acaatttatt gagtttaatg aagaattgct ctttgaatag
     2701 ctgaatatgt tcaatctgaa actggctgag tatctggtac tgtacaacag taaaattctt
     2761 cataaatcgt ttaaactaca aaaatcagta gaatatatta tttccactgt caggcggaaa
     2821 atttatagaa aggatacaaa catatgccaa taataaacaa atcggcatca aa
//
LOCUS       DAZFTN010000272         2852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFTN010000272 DAZFTN010000000
VERSION     DAZFTN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..260
                     /locus_tag="ACHKV2_005230"
     CDS             <1..260
                     /locus_tag="ACHKV2_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6942913.1"
                     /translation="KKIMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENGIIPE
                     QDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            374..949
                     /locus_tag="ACHKV2_005231"
     CDS             374..949
                     /locus_tag="ACHKV2_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6942914.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            1660..2310
                     /gene="nleG7"
                     /locus_tag="ACHKV2_005232"
     CDS             1660..2310
                     /gene="nleG7"
                     /locus_tag="ACHKV2_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO6942915.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            2460..>2852
                     /locus_tag="ACHKV2_005233"
     CDS             2460..>2852
                     /locus_tag="ACHKV2_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO6942916.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          875 a          551 c          641 g          785 t
ORIGIN      
        1 taaaaaaaat tatgcagcgt ctgtgcggtt gcggaaagca tgatgaccgt gaaaacgggg
       61 agttacttac agcacagctg cgactgggac cggcagacat tctggagtcc gatgagaatg
      121 gtattatccc ggagcaggac agggtaatca cgcaggtggt gatactggat gcggataaaa
      181 agcagataca gtgcgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa
      241 atattggcgg gatgaaatag cccgacagct tcacaaaaac cggagtccgg ctccggtttt
      301 tgttgtcatg tatgggggct gtttgttatg actccctgtg tttggaatga atatttaaat
      361 agggagtttt gtcatgccgt taacatcagc tattgcatcc aattcatttt ccaccggaat
      421 gcaggttctt cgtgctcaaa tggccgccag tggcggtgga gaaattacag taggcgggca
      481 gacggtccgt atcacatata gtgaaacgga tggtcgcttt ctggcgagtg ggggcaataa
      541 cagtttgctt tctggattat tacttacagg gcttaatggt ggtcctgaag cgctcaggga
      601 tataatgtta agaatggttt caggttcagg taacacacaa tcacatggtg atattgaggg
      661 gaaaatatca caatgtaagt tttctgttaa tacggagagc cttcagtgtc catccgaggc
      721 ggttcgatgc ccaattatac tggataaacc agaagaaggt gtgtttgtta aaaattcaga
      781 aggttctttg gtttgtacct tatttgattc ggtttctttt tctcatttgg ttcgcgacgg
      841 tgggaagcac ccgctaacac gagaaccaat aacgtcatca atgattgtaa gtcaagaaca
      901 atgtatttat gaccaaacca aaggaaactt tgtcataaag gataagtgaa ataaatatta
      961 cccaagctat atgttaactg ccagttgctt atatgaaatg ctacagatgt tcagggtata
     1021 aggatgtggt aattggtgta ctggatgcag ctaaaaagca tatacagtga gtggtgaggc
     1081 cgctgcaaat ctggcgtgct gacggggaaa tattggcggg atgaaatagc cgacggattc
     1141 acaaaaaccg gagtccggct ccggtttttg ttgtcatgtc agggagatgt ttgttatgaa
     1201 gcccagagga aatatttatc tgtatgaagg aatatggtaa tgcctggatt agtatcatat
     1261 atatcatcga cttcattcgc gaatgagatg gcggagatgc gtcagcaggt aatggaaggg
     1321 cagattggtg gatttctcct gggaggggag agagttagag tttcttattt atttcaattg
     1381 cattaatatc ttgaatggat tacatagagt taatgtatcc agtactccct attctcttgc
     1441 atataataca tgttgcaact tacatctcag cgctatgaaa aaacaccacc tctctcttta
     1501 tgaaattctg gatttaccaa gcgctaattt atcatttcaa tctaccttta agtattgcat
     1561 ttatctccct acgaggtcat actttaggaa gttaaatatg aatgataata tacctacagc
     1621 gcgaaatcac aaacaatcga cttgtattac agaaaaaaca tgcctatatt tttaaacttc
     1681 acagcaggaa gtatcttacc tgagaatgag ctagcatctt tacgttatat tgtgcagcaa
     1741 aatcaaaatg atactgtaat cataaaagaa cgttataaaa tggatatccg ttatatcgaa
     1801 tcagtcaatg gttttacagt aaatcctgta tgcagtaatc atttctccat atttatggcg
     1861 agacaaaaca ctattgctcg caacctggaa cagcagatca acaacggacg aagttttgca
     1921 caaatatctc aggattttat gcttcaatta tcttcaaata taggatggaa aaaaggggcc
     1981 gaaaacgccc ttaaaaataa aatccattct cattcatttg ttgtaaatcc tgatgaattc
     2041 tcttgcgaca cacaatttct taagtgccca ataacattat gcgttccaga aaaaggggtt
     2101 tttgtcaaga atgcactgaa ctccaacata tgcactcttt atgataagtc tgcgttcatg
     2161 aatctcacaa gagaacatct accccaccct ctcagcaggg aaaagatagt aaaagaaatg
     2221 attattgaaa ggaatatgtg ttattttgac accataagtc agcatttcat aattatggat
     2281 gcagaccaac agaaacagca ttgtaaataa aatgtaataa ttacatacta ttagtgattc
     2341 tcatgcatcg taagcggctc gccagaaccg tattgatatt tactgaggag cctgtacata
     2401 gatttgtgta attgcctgat tttgatatgt tcaatccaac atcaaaagca ggttaattta
     2461 tggacgaaaa acagttgcag gctctggcta acgaactggc caaaaatctc aaaacccctg
     2521 aagatctcag ccagttcgat cgcctgctga agaaaatcag cgttgaggca gctctcaacg
     2581 ctgaaatgtc ccaccatctg ggctacgata aaaaccagcc taaaccgggt gccaactccc
     2641 gcaatggcta ttccacaaag accgttatca ccggtgatgg ccacctggaa ctacgcactc
     2701 cgcgcgatcg tgatggctct tttgaaccgc aacttgtgaa gaaaaaccag acccggatca
     2761 ccgggatgga taaccagatc ctgtcgttgt acgccaaagg cctgacaacc cgcgaaatag
     2821 cggctgcgtt taaagagctg tatgacgctg at
//
LOCUS       DAZFTN010000273         2846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFTN010000273 DAZFTN010000000
VERSION     DAZFTN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHKV2_005234"
     CDS             complement(<1..296)
                     /locus_tag="ACHKV2_005234"
                     /EC_number="3.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6942917.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFS"
     gene            complement(299..1264)
                     /locus_tag="ACHKV2_005235"
     CDS             complement(299..1264)
                     /locus_tag="ACHKV2_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFO6942918.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(1267..2385)
                     /gene="gmd"
                     /locus_tag="ACHKV2_005236"
     CDS             complement(1267..2385)
                     /gene="gmd"
                     /locus_tag="ACHKV2_005236"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6942919.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(2405..>2846)
                     /locus_tag="ACHKV2_005237"
     CDS             complement(2405..>2846)
                     /locus_tag="ACHKV2_005237"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6942920.1"
                     /translation="ATQDYRFPGYFNELMVLAKKLGIESKIKILGHIPKLEQIELIKN
                     CIAVIQPTLFEGGPGGGVTFDAIALGKKVILSDIDVNKEVNCGDVYFFQAKNHYSLND
                     AMVKADESKIFYEPTTLIELGLKRRNACADFLLDVVKQEIESRS"
BASE COUNT          874 a          601 c          492 g          879 t
ORIGIN      
        1 gaaaagtctc cccccatact attgtcttcg tagaagtgct gccagatacc ataaaactta
       61 cccacagaga gaggcaaacg aattcctagt tcaatttctg tcaatcgttc aaaggctgtc
      121 tgcaattttt catctttcaa caccctacca ccaggaacga accaatagcc ctgtgccggg
      181 cggttgattc gttttcctag caaaatttcg ccaaactcgt tttccacaat caaatctata
      241 gaaataagag gagtagacct tacaattgtg gcaaagtctt gggaatgtaa aaacattatt
      301 acccccgata ttgaagttgg ttttcaagaa accagttgta tgtattttca agacccttgt
      361 gaagggtaat tttatgattc caacctagtt gatgaagaag cgttacatca agtagttttc
      421 gaggggctcc atcgggcttt gttgtatcga acgtaatatg ccctttataa cctacaactt
      481 ttgctattgt ttccgcaagc tcacaaatcg tgcagtcaat acctgttcca atattgatat
      541 gagacaacat tactttagta tttttttgcc atatatcgta tggcatctcc atgacataaa
      601 tgcttgcaga agccatatca tctacatgta agaattcacg ctttggagta ccacttcccc
      661 aaacaacaac attcggagaa ttgttttcca cagcatcatg aaagcggcgc aaaagcgccg
      721 gaatcacatg agaattactt ggatgaaaat tgtcatttgg accataaaga ttggttggca
      781 ttactgaacg gtaatcacgc ccaaactgac ggttataaga ttcacataat ttaatacctg
      841 caatttttgc gatagcataa ggttcatttg ttggctcaag tttcccttgt aataattcgt
      901 cttccataat cggttggtgt gctaacttag gataaataca cgacgaaccg aggaaaagca
      961 gtttatttac attatttttg tgggcagcat gaatgacatt cgcctctatc attatattct
     1021 catatataaa atcggcagga taagaactgt tagctaaaat acctccgact tttgctgctg
     1081 ccaaataaac ctggtcgatt ttctgtgaag aaaaaaaatc caaaacagcg ctactatcca
     1141 acaagttcaa ttcatcccga gtacgtaaaa ccaactcaac atcatcacgt tgtttgaggc
     1201 gtcgggtaat agctgatcca accattcctt ggtgaccagc aataaaaata cgttgcttat
     1261 tcatcatcat tccagagcta agcttacaga aaaaccatgc gattttaaaa gagaatgttt
     1321 tttagcggct tcaagatctt tggcaaccat ttcagaaatc atttcagcaa gagtaatttc
     1381 tggtctccaa ccaagtttga gattagcttt gctcggatct ccaagcaaag tatcaacttc
     1441 agctggtcgg aaataacgag gatcaacagc aacaatgaca tcacctggtt tcacacctgg
     1501 agcatcctgt ccttcaaccg aatctacaat gcctttttct tcgattcctt taccaacaaa
     1561 gctcatctta ataccaagtt gtgctgctgc catttcgaca aactgacgga ctgagtattg
     1621 gactcctgtt gcaatcacaa aatcttcggg ttgctcctgt tgtaacatca accattgcat
     1681 tctaacataa tcttttgcat gtccccaatc tcgtaacgaa tccatattcc ctaaatacaa
     1741 acatgattcc aagccttgtg caatatttgc aagtcctcga gtaattttcc ttgttacaaa
     1801 cgtttctcca cggcgtggag attcatgatt gaacaatata ccattacatg cataaatacc
     1861 atatgactct cgataattta ccgtgatcca atatgcgtaa agttttgcaa ctgcataagg
     1921 ggaacgagga taaaaagggg tggattcttt ttgagggatt tcctgaacaa gtccatataa
     1981 ttctgaggtt gaagcttgat agaaacgcgt tttgttttcc aatcctaaaa agcgaattgc
     2041 ttccagtaaa cgtaatgtac caattgcatc gacatcggct gtatattctg gagactcaaa
     2101 agaaactgct acgtgactca tagcagctaa attatatact tcatctggct gtacctcctt
     2161 tagaattcta gtgaggttag atgaatcagt cagatctcca tagtgcaagt gaaaatttgg
     2221 gttagaacca tgtggatctt gataaatatg gtctatgcgt tctgtattaa aagatgaggc
     2281 tcggcgtttg ataccatgaa cttcataccc tttatcaagc aaaaactcag ctagataaga
     2341 tccatcttgt ccagttacac ctgtaataag agcgacttta gtcattatat acctcttgaa
     2401 tatattaaga tcgggattca atttcttgtt tcacaacatc taaaagaaaa tctgcacacg
     2461 cattgcgtct tttgagaccc aattctatca gagttgtagg ttcataaaaa attttagatt
     2521 catcagcttt taccatcgcg tcatttaatg aataatggtt ttttgcctga aagaaatata
     2581 catcaccgca attaacttct ttattgacat ctatgtcaga tagtataact tttttcccta
     2641 atgcaatagc gtcaaatgtt accccccctc caggcccgcc ttcaaataag gttggttgta
     2701 ttacagcaat gcaatttttg attaattcaa tttgttcaag tttaggtata tgccctaata
     2761 tcttaatttt cgattcaatt ccgagctttt ttgccaaaac catcaattca ttaaaatatc
     2821 cagggaatcg ataatcttga gtagct
//
LOCUS       DAZFTN010000274         2832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFTN010000274 DAZFTN010000000
VERSION     DAZFTN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(167..1759)
                     /gene="lsrK"
                     /locus_tag="ACHKV2_005238"
     CDS             complement(167..1759)
                     /gene="lsrK"
                     /locus_tag="ACHKV2_005238"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO6942921.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1838..2791)
                     /gene="lsrR"
                     /locus_tag="ACHKV2_005239"
     CDS             complement(1838..2791)
                     /gene="lsrR"
                     /locus_tag="ACHKV2_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO6942922.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
BASE COUNT          720 a          775 c          644 g          693 t
ORIGIN      
        1 taatgcttta agaaaaatag caatttcccc ttgaatatag tactggtgat gaaacgatga
       61 atctgcaagc tggcatttaa caagccagct ctaaaagaag ggaaataaga ataactatac
      121 tcaaaaacta acagccacgg tcatcatgat gtggctgtca atgaaactat aacccaggcg
      181 ctttccataa cgacgtcgtc agtccatgat caaccagccc cagctgatcc tgataaactg
      241 cctgccactt atcgcgtgaa tcctgataaa gttcatgctt ttccgggtct ggtgtgtgcg
      301 tccgttccca gcgaaccagg cgttctccgg tttctgccat tgatgaaaaa attccggcac
      361 cgacgccagc tgcaatggca catcctaatg cagtggcttc tttgaccacc ggaatattga
      421 cgggtaatcc cgagacatca gcgagaattt gactccataa tttccctttt gaacctccgc
      481 ctgcaaagac taacgatgaa ggatgaatag tcgaaaaatc agcaatttgc tgcaagttac
      541 acgctgatac aatcgccgca ttttcttcca gcgcacggaa caatgtcgct ttgttacatt
      601 tatccggatc aatggacaaa ttaataaagg aaggcgcagc gtgataccag gttttaaagc
      661 gcattctgtc ggagaagatc ggcattacgc cccacgaccc aggcggcacc cgactggcca
      721 tctcttccag cagcgtatag gtgtcgatgc ctaaacgttc ggcaatcagt ttttcttcgg
      781 cacagaaagc atcgcggaac cagcgcatgg tgagtccggt aaaaaagctt atagattcag
      841 cttgtaccat gccaggaata acatgaggat taacgcgcac gttcatttct gggtctgtca
      901 ccggcgcggc taaatttaca acttgctgcc agaatgtgcc gccaagaacc gcggtttgtg
      961 ccggacgcac aacgcctaac cccaggcaac caagctgcac gtcgcctcct ccaacgacca
     1021 ccggagtgcc caccttcaga ccgcagagtt ccgccgcttg tgaacttacc acgcccagca
     1081 atgtgccggt ttctttgaca ggagaaagaa tatcggcacg caggccagcc atatccagca
     1141 atgcaggttt ccagtcacgg atggttagat caagaagtcc cgtggtgcca gcgttagagg
     1201 gatccaccgc aagttcgccg ctgagcatat aggccagcca gtcgctgatc atggtgatgg
     1261 ttgatgcctg acggtaaata tcggaacgat ggtgcgccag ccaaagtaat ctggggatgg
     1321 cacttaaagc cagtgtttgt ccggtcgcgc gataaacttc gttttcaaag gtattgttgt
     1381 gcagttcttt aagttcgcta acttcgcgcg ccgctctggc atccacattg gcgcaggccc
     1441 agatcggtgt tccttcattg ttatataaaa caatgccttc acgcatcgaa catgccgaaa
     1501 cggcagcgat atactccggg gctatgccgg cgttgtgcag cgcctggcgc atacactcac
     1561 acgccagttg ccagtttttg ttgagatcaa attccataga accaggaacg tccggtactg
     1621 ccagatgccg ccactccgcc tgtcccactg ctatttgatt gccttccagg tcgaatatca
     1681 cagcccgaat acttccggtg cctgcatcca gcgccatcag gtagtacttt gattctgaag
     1741 gggtaaagag tcgagccatt agattatcct cggcttatgc gttctgtctg ttcctctata
     1801 cgttctccat cattcccggt aataaggtca tgcaaattta actacgtaaa atcgccgctg
     1861 ctgtgtcctg atcggtaacc agtgcgttga tataaccgcc tttcattgca gcggcaattg
     1921 cttcggcttt attttctccc cctgccacgc caacccggac gggtatggtc ttcagcgcgc
     1981 ttaaaggtaa gccaatcagc tcgttatgta ttttaatgtc cgtgacaacg tcaccttttg
     2041 catcaaaaaa gtagcctaaa atgtcgccaa ccgccccttt tcggccaatc attaactgtt
     2101 cgccctggct gatataaccg gagcgaatga tcgtcgcgtc gtcttgttga ctaacggccc
     2161 caatgccgac aatcgccaca tccgctgctt gcgcggctaa cagaacatct ttgacacaat
     2221 tttcattttt tagcgtacgg gcaatgtcag cggaggatgc ccgcaacgga gccggaataa
     2281 tattcacact gcacgccgca ttaagctgtc cgatacccgt catataagaa ccgacgccac
     2341 cggagagcgt gaccaggcga atttgctgtg acgaaataaa accacttaag cgttgcagcg
     2401 tattcatggt tgcctcgcca aaaccaatcg ccagcatctg ttgtggttga agtaaactca
     2461 tcaacatatg cgccgcgcct atccccagtc gcccgccgac atcagcatcc gcaagcccag
     2521 ggatcacccg gacatgttgc agcgaaaact gacgacgtaa ttgtgtttca tattccagac
     2581 agccttcaaa gcgagaatta atctgcacgc gaatgatgcc ggactgatgc cctttctcca
     2641 gtaatcgcga cactttcaaa cgggtcaggc cgagacgatc gctgatctca ctctgggtca
     2701 gcccgtcgtg gtagtaaaac cacgcgatcc gcgcgacctg ttcttcttca cacattccct
     2761 gttctgaaat taccgaatcg ttgattgtca taattcattc ttcactttga acatatttaa
     2821 atctttaatg ca
//
LOCUS       DAZFTN010000275         2813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFTN010000275 DAZFTN010000000
VERSION     DAZFTN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..803
                     /gene="paa"
                     /locus_tag="ACHKV2_005240"
     CDS             45..803
                     /gene="paa"
                     /locus_tag="ACHKV2_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO6942923.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1139..1294
                     /gene="hokD"
                     /locus_tag="ACHKV2_005241"
     CDS             1139..1294
                     /gene="hokD"
                     /locus_tag="ACHKV2_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6942924.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1515..1772
                     /locus_tag="ACHKV2_005242"
     CDS             1515..1772
                     /locus_tag="ACHKV2_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942925.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1842..2120
                     /locus_tag="ACHKV2_005243"
     CDS             1842..2120
                     /locus_tag="ACHKV2_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942926.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2122..>2813
                     /locus_tag="ACHKV2_005244"
     CDS             2122..>2813
                     /locus_tag="ACHKV2_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6942927.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          739 a          655 c          739 g          680 t
ORIGIN      
        1 tgtttataac tttatttcat gataaccaat aaaaggagtt ttttatgagg aacataatgg
       61 caggtttttt aatattcctg tcttctgctg cttatgctga tatcaatctg tatggtcctg
      121 gtggcccgca tacagccttg cttgatgcag ccaaacttta tgccgaaaaa acaggtatta
      181 tagtgaacgt tcattacggc ccacagaaca aatggaatga agatgccaaa aaaaatgcag
      241 atatcttgtt tggcgcatca gaacaatctg ctctggctat cattcgggac cataaagaca
      301 gcttcagtga aaaagatatt cagcctcttt atctgcgaaa aagtatttta ctggtaaaga
      361 aaggtaatcc taaaaatatc cggagtattg acgacctgac cagacctggg attggcgtaa
      421 ttgttaatga tggtggtggt accagtaata catcaggcac tggcgtctgg gaagatattg
      481 ccggacgtaa agggaatata gaaactgtcg ccgcaatccg aaaaaatatt attttatatg
      541 cgcccaatag cggaactgca cgtaaggctc ttgagaatca gcctggagca gatgtctgga
      601 taacctgggc tgactgggca gccagtaatc cagaaattgg tgatgtcgtg gaaatagcgc
      661 cagactacgt gatatggcgt gatatgaaca ttacagtacg tcaggatgca aatgatgaaa
      721 cccgtcgatt tgcagaatgg ctacaaaccg atgaagcggc gcctgcattc aaaaaatatg
      781 gctggaccag gaaaggcact tgacatcctc gtccttcagg acgtggattc tttttccgga
      841 tgccgcgcca gcggcatgta ggggcagctc acaaaacgga aaaaattgta cgctaagcct
      901 cgccaggtga actgaattca ttccgatatg ggaattccca tatcgggcga aaacggtttg
      961 ctgtaacggc agagttaagt aggattgctg cgggtgcttg aggctatctg cctcgggcat
     1021 gaacaccaac ggcagataga taaaagcccc acccgactat aaatcgaagt gaggccccta
     1081 tatgctcgtc acatatagat tgcctcttac ggaccgaaag gtcaaggaga agcaggctat
     1141 gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc tgtttaatcg tcatagtgac
     1201 ggcactggta acgaggaaag acctctgcga ggtacgaatc cgaaccggcc agacggaggt
     1261 cgctgtcttc acagcttacg aacctgagga gtaagagacc aggcggggga gaaatccctc
     1321 gccacctctg atgtgtcagg catcctcaac gcacccgcac ttaacccgct tcggcgggtt
     1381 tttgttttta ttttcaacgc gtttgaagtt ccggacggcg ccggaataga atcaaaaata
     1441 cttaagtagc gcgcagggag aagagggatg gaccccgaac aggggagtgc tatttatctg
     1501 gaaggattct gttgatgaga atcgaagaat tacgtgaaat ttttagtgaa gatggcctct
     1561 atactgtgcg cgttgagaag ggcgctattg tcagccactg ccgtattaaa tgtttacagt
     1621 ctcaacaaag gaagagtgga gctgcgttaa ttcattttgt ggatgggctt gtgacggatg
     1681 gttttatttt gcgtgcaaat gaatttgtca catcgttgcc gtctctgaaa gaagctggga
     1741 ttaaggctgg tttttctgct tttgaagatt agtgaattca tctacaattc agcgcagggc
     1801 tgaacccctg ttgagtaaca ctgtgccacc ggagaaagcc gatggcgcaa aattccagac
     1861 tacacaattc tgataattca gccgtctttg ccagcaggca cgggcggcgt tctcatgcat
     1921 tcaaatctga ctggttccgg cacgccccat gcactgaaga acaggccgaa tggctgattc
     1981 agaactaccg cagacgtggg tatgagttta ggaaagccct cagcctcgat tatcgtcact
     2041 ggataatcta cgtcaggctg ccgtactccg agcgcccacc gcgtccgtcc cgcacattcc
     2101 agcaacgcat ctggaggtaa cgtgcgggta ttacttcgac ctgttccggt accggaactt
     2161 ggggtggtgg tccttaaacc aggccgtgaa tccatgcagg tattccataa ccctcgagtg
     2221 cttgtggagc cggagccgaa aagcatgcgc ggtctgccgt ccggcatcgt ccctgccgtt
     2281 cgccagccgc tggcggagga taaatcatta ctgccgtttt tcagcaatga gcgtgtgatt
     2341 cgtgctgttg gtggcgctgg tgcactgtct gactggctgt tgcgtcatgt caaatcctgc
     2401 cagtggcctc atggtgacta tcatcacagt gaaaccgtta ttcaccgtta tggtaccggc
     2461 gcaatggtgt tgtgctggca ctgcgacaac cagctgcgtg accagacatc cgaatcactc
     2521 gagcaacttg ctcatcaaaa cctgtcagca tggatgattg acgtcatcgg tcacgcaata
     2581 agcggtacgc aggagcgtga attatctctg gctgaattat cctggtgggc ggtccgcaat
     2641 caggtggcgg acgcgctacc ggaagcggta ttacgtcgtt cgctggggtt gcgtgcggaa
     2701 aaaatccgct caatgtaccg tgaaagcgac atcgtaccgg gagagcagac cgccaccatc
     2761 atactgaagc agcgcacaaa aaatcttgcg ccgctgcctc acgcccacca gca
//
LOCUS       DAZFTN010000276         2781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFTN010000276 DAZFTN010000000
VERSION     DAZFTN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            191..1144
                     /gene="ompT"
                     /locus_tag="ACHKV2_005245"
     CDS             191..1144
                     /gene="ompT"
                     /locus_tag="ACHKV2_005245"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO6942928.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1331..>2781
                     /locus_tag="ACHKV2_005246"
     CDS             1331..>2781
                     /locus_tag="ACHKV2_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6942929.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENI"
BASE COUNT          913 a          589 c          554 g          725 t
ORIGIN      
        1 aaaatttcca gcatattaaa ccacaacaat aatacaacac ttggcaagct gcccataaag
       61 aatgaataaa acgaaacaat aattcaaact taaacaaacc ataaacagtt aaacaatata
      121 caattgactc attaagttag atataaaaaa tacatattca atcattaaaa cgattgaatg
      181 gagacctttt atgcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag
      241 ctcttttgct tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag
      301 tcttggaact ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg
      361 aaaagtcagt caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa
      421 ttgggatttg atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg
      481 aggtggcaat atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga
      541 tgaaagtaga caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa
      601 aggctggctc ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag
      661 ccgttatagc tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag
      721 agatgatatc ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa
      781 aatgccctac attggcttga ctggaagtta tcgttatgaa gattttgagc taggtggcac
      841 atttaaatac agcggctggg tggaagcatc tgataacgat gagcactatg acccaggaaa
      901 aagaatcact tatcgcagta aagtcaaaga ccaaaattac tattctgttt cagtcaatgc
      961 aggttattac gtaacaccta acgcaaaagt ttatgttgaa ggcacatgga atcgggttac
     1021 gaataaaaaa ggtaatactt cactttatga tcacaatgat aacacttcag actacagcaa
     1081 aaatggtgca ggcatagaaa actataactt catcactact gctggtctta agtacacctt
     1141 ttaacgaagt taaccagatt ttctccccgg tggtattgta taaccccggg gaatttgttt
     1201 acagttacaa gtagacaata tccactaatt ttgtaagcat ttaacctatg tcataacgtg
     1261 taatctctta ccgcaacaca agacctcaat atattcaaat tttatcttta ttccgatatt
     1321 cagagacaca atgcgaaaaa taattactca tttcaaagtt gttttaacgt tacttctacc
     1381 agtaaccgta tctgcccagc agatacagtg gcaatcctgt atggccagtc aattcaacca
     1441 ctggtttggt gaggaaaaac cgtctcctga cttactatgt ggttatttgt ctgttccatt
     1501 aaaatataca gacacaggcg gagatgcttc ttatgaaaaa aaatcacaag tcaaactagc
     1561 gttgacaaaa ttgccggcaa aaagcaagca taaaggaagt atcctgataa taagtggtgg
     1621 tcccgggtta ccaggcataa atccttatat taactttgac tggccagtca caaatcttcg
     1681 tgagtcatgg gatattattg gatttgatcc tcgaggcgtc gggcagtcca ctccgacaat
     1741 aaactgccgg caatcagata cagagactca ggaaaacata accgaaaagc aacaagtatt
     1801 aaataaaatt aatgcctgta tccataatac cggagccgaa gtcattcgcc atatcggctc
     1861 taacgaggct gtatacgata ttgatcgtat taggcaagcc ttgggggata aacaactgac
     1921 agccgtggcg tattcgtatg gaactcaaat tgcagcctta tatgcagaac gttttcccta
     1981 caacgtaaga tctatcgttc ttgatggagt cgtcgatatc gatgacctgg aggacaactt
     2041 cacatggcaa ctcaaacagg cacagagtta tcaggaaacg tttgatcgct ttgcatcctg
     2101 gtgtgcgcgt acaaaaagtt gcccgctttc ttcagacaga gataaggcaa taactcagtt
     2161 ccatgagcta ttatcaaaat tacatcacaa acctttatta gacagtaagg gagaaaatat
     2221 atcttcagat gaactcatat cattaacaac agaccttctg ctatggcgtt catcatggcc
     2281 aacccttgca actgccatac gccagttctc tcaggggatt gtcagtaatg aaattgaaac
     2341 tgcgctcagt gctccgatag cctcagaaga gtcaagtgat gcttcggggg taatcctttg
     2401 tgtagatcag ggggatgagc aattaacacc agaagagcga aaattccgaa aagaggctct
     2461 tgcgaatgcc ttcccggcta ttaactttga caatggacgt tccgattcac ctgatttttg
     2521 tgaattatgg ccaatacata gcgacctgaa caaaactcgc ctgaaaaata ctgttctgcc
     2581 ctctggttta ctgtttgtag cacacaaata cgacccaaca acgccctgga ttaatgcccg
     2641 taagatggca gagaaatttt ccagcccgtt actaacaata aatggtgatg ggcatacatt
     2701 agctctcacc ggagttaatt tatgtgtaga taaagcagtt gtacatcacc tgatcactcc
     2761 acaaaaaata gaaaatatat a
//
LOCUS       DAZFTN010000277         2763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFTN010000277 DAZFTN010000000
VERSION     DAZFTN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            23..241
                     /locus_tag="ACHKV2_005247"
     CDS             23..241
                     /locus_tag="ACHKV2_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO6942930.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            243..800
                     /locus_tag="ACHKV2_005248"
     CDS             243..800
                     /locus_tag="ACHKV2_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6942931.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1091..1246
                     /locus_tag="ACHKV2_005249"
     CDS             1091..1246
                     /locus_tag="ACHKV2_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6942932.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1366..1710
                     /locus_tag="ACHKV2_005250"
     CDS             1366..1710
                     /locus_tag="ACHKV2_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO6942933.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1832..2104
                     /locus_tag="ACHKV2_005251"
     CDS             1832..2104
                     /locus_tag="ACHKV2_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942934.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2106..>2763
                     /locus_tag="ACHKV2_005252"
     CDS             2106..>2763
                     /locus_tag="ACHKV2_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6942935.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     T"
BASE COUNT          725 a          636 c          748 g          654 t
ORIGIN      
        1 ccgcccgaaa gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag
       61 cagaacaaca gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc
      121 gctattactg gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga
      181 tgtgacatgg gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg
      241 atatgagcac tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga
      301 gcgactcggc gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg
      361 cgctggcatc actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc
      421 ttacctgcca tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg
      481 tcgccatgct tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc
      541 gggaaatctc ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta
      601 ctgagcgaat gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg
      661 ctgggccatt aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag
      721 cagttagtcc gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc
      781 cgcaggaggt gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc
      841 ggtggcgctg gtggtgtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg
      901 atagttaagt agaattgctg cgggtgcttg aggctatctg cctcgggcat gaacaccaac
      961 ggcagataga gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc
     1021 tgaggctcaa tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga
     1081 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
     1141 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg
     1201 tcagacggag gtcgctgtct tcgtagacta cgaatctgag aagtaagaga ccaggcgggg
     1261 gagtaatctc ccgccacctc tgatgtgtca ggcatcctca acgcacccgc gctttaccat
     1321 actgaaaatg ctgtttgaat gttcatctct gaaagaggac tatgaatgaa aaaggtattg
     1381 attgcagcac ttatttccgg tgtgtctttt ggcgcttttg cacagcaggg tggtttccag
     1441 gggccagaag cagagcgttc aacagtagcg caggcaaaag aactgaagga tgatgcatgg
     1501 gttatccttg aagggagcat cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac
     1561 aatagcggga caattgtcac ggatattgat gacagcgtat gggccgggca gaatgtttct
     1621 ccgaaagaca aagtaagaat tgagggtgaa attgataaag acctgagcag tgttgaagtt
     1681 gatgtaaagg cactgaaatt attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg
     1741 acgggttttc tgtttgtaca ttccggcgta ttgccttaca attcgcgcag tcagcctgaa
     1801 caactgacac ctgctgtcac cggagaatcc gatgacacaa cacataaaat cccacaattc
     1861 tgaagccgac ccggaaatta agcaggggag gcgttttcgt gcgcctcagt atggctggtt
     1921 tcactatctg ttctgtacga tcgatgaggc agatatgctt caagaggcgt atctgcgtcg
     1981 cggtgtccgt gtggagcgga gtctgaacgc tgatcgtctg acctggaccg tttctgtata
     2041 tcttcctgtt cgtgcacatc tgccacggac acatgcctgc taccgtcagc gcgtctggag
     2101 gtaatgtgcg ggtattactt cgacctgttc tggttccgga actcgggctg gtggtcctta
     2161 agcccggtcg tgaatcattg ccagtttttc atcgcggcag ggtgctggtg gagccggaac
     2221 cgaaaaacat gcgggcgctg ccatctggag cggttcctgc tgttcgccag ccgctggcgg
     2281 aagataaatc actgctgcca tttttcagcg atgagcgggt gattcgtgca gctggcggcg
     2341 ctggtgcact gtctgactgg ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg
     2401 attatcatca cagcgaaacc gttattcaca gttacggtgc tggcgcaatg gtgttgtgct
     2461 ggcactgcga caaccagctg cgcgaccaga cctccgaatc acttgagcaa cttactcaac
     2521 aaaatctgac agcctggatg attgacgtca tacgccatgt aatgaatggc acgcaggagc
     2581 gggaattatc gctggctgaa ttatcctggt gggcagtctg caatcaggtg gtggacgcat
     2641 tacctgaggc agtatcgcgt cgctctctgg gattaccggc ggaaaaaatc cgctccgtat
     2701 accgtgaaag cgacatcata ccgggagaac agaccgccac cagcatactg aagcagcgca
     2761 caa
//
LOCUS       DAZFTN010000278         2661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFTN010000278 DAZFTN010000000
VERSION     DAZFTN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..407
                     /locus_tag="ACHKV2_005253"
     CDS             <1..407
                     /locus_tag="ACHKV2_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942936.1"
                     /translation="NSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAE
                     FLSRKGNPFIGFVVLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVI
                     LENKGPTFQIKINDANKIHNLNKNGSSGFYPV"
     gene            complement(817..1089)
                     /locus_tag="ACHKV2_005254"
     CDS             complement(817..1089)
                     /locus_tag="ACHKV2_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6942937.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(1095..1637)
                     /locus_tag="ACHKV2_005255"
     CDS             complement(1095..1637)
                     /locus_tag="ACHKV2_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO6942938.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMED
                     YARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTSALA
                     PFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQSSY
                     TPAQQLRFQQRLDYINGMQE"
     gene            complement(1634..2386)
                     /locus_tag="ACHKV2_005256"
     CDS             complement(1634..2386)
                     /locus_tag="ACHKV2_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO6942939.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
BASE COUNT          653 a          620 c          615 g          773 t
ORIGIN      
        1 aaaattctaa agataacaaa gtgcgatttg acgaagatac atatgacttt aatatcaatg
       61 ggaaacgccg aggaatgttt ggtaaaggta cccgtgcagt aatgcatgct atatttacca
      121 tttgctttgc ggaattttta tcaagaaaag ggaatccatt cattggattt gttgtgcttg
      181 atagtccatt agtgacgcac tttgataaag atagaggggg ttctctatcg gatgttaata
      241 gcgtcagtct cagtgattca ttttatcacg cattaatcaa aagggactat aatttccaga
      301 ttgtaattct tgagaataaa ggtccgacgt tccaaataaa aattaatgat gcaaataaaa
      361 tccataactt aaataagaat ggaagctctg gtttttatcc tgtttaatac attaaaaata
      421 taactgctaa ttggggtgct cgaaattata tttcgtttcg agcgctcagt tcatttctaa
      481 ataatttgca aaattatttt gggtactttc gcctctacac ttccttcggg tggttccaca
      541 ataatggatc tcaccggcgt ttgatatttt ataatctaac cccttagcgc gcgcagcccc
      601 ccacgcctgc tcgcttcgct taacagactg gttttcatgc attccgcaaa tcgtctcaga
      661 agccaccaca caagggcttt cgcgtcaaaa atggtgcatg agaatcatgc gttttcatgc
      721 gctatagaca tgcactcatg cgctctcagg ccagccaggg aaaaggcgta aaaaatccca
      781 gtactggacc gggattacgt gggctttttt tgctaatcag acaggaattt gctgacggct
      841 tgacagcttt gacgcggagc catagcgatt gagagtttgc tgttgttttt ccggcatttg
      901 tgctttctct gattcctgtt cattgcgccg gggcttcatg attatcgtat ccacactttc
      961 cagtgcggta aatgtgtagg aacattcaag attctggcac tgataccatg agcgtttaac
     1021 cgatggtgct tcataggcgg aggttctggc gtgtgccgtc gtaccgcatt cgggacattt
     1081 aagtgccatt aatattactc ctgcatgccg ttaatgtaat ccagacgttg ctgaaagcgc
     1141 agttgttgtg caggggtgta agagctctgg caatcacgaa ccatcatggg gtcaggcatc
     1201 acaccggatt catccagaat catacgataa tccccggcaa tttcaggcgg tgttattgcc
     1261 agctggcgaa ccagagcatt gcgtaatata cgggacgcaa cgtcaacccc tccgcgcccc
     1321 ttcaggaacg gagccagtgc agatgtcagg gctgctccat tcgcctgcat aaaattgctg
     1381 agagcgtctt ctgtgcaggc ctccatcagt tgatgctgcg agtaaatgca ttcgcgcgca
     1441 gcacagttaa tttgccattt cagcacatcc agatgttccc gctgtaattc gagctgtcga
     1501 gcataatctt ccatggcagt gaggagtgat tctatctgtt gttcaacggc ttttcggctc
     1561 tcactatgtc cagcccacag aatacgggca tttctaaaac tgataagcgc ttggtctgga
     1621 gtgactcggg ccatcatact gctttctcct tctcagcacg ttcctgacga accttcattt
     1681 tatgcatagc tgctggagag agtttattac tgaaatccag acgggccgca taatccgggg
     1741 caactcccgc cagtttaaac acggggtcct gttcgggcat cgcatcgtta gtcagagccg
     1801 gtctggtgat atgtttatga ataaaatctt tcagcagtgt gtccgggtca ttaactgaat
     1861 gcaccacacc gacaactgcg ctggcttccc tgcccattgt tgatttgagc aggcttagtg
     1921 tctggattag tgcttttcca tgcgactgca taaaatcttc ccagatttgt tttgcgcgaa
     1981 tacccacaag ggtgtcgtgc gcgtggatat actttccggc taattccgca gcctcctgcg
     2041 gcaaaagtgc attttcgctt tcctgagccg ccagcagctc atcaaaatcc tcaagcgttt
     2101 ctcgtcccag cgcaatttcc gtgcgcagtt ttttcatttc ctttgagact acgccctgac
     2161 tttcacgaaa tagcgtgcgc cactcctcat tcagggcgtt cgtcgtggct tccatttcag
     2221 cgcggcgctg gcggattgtc gcaatattat ccgctgccgc tttttgctga cgacgtgctt
     2281 caagccatgc agatttggtg gcattcactt tctcaagcgc ctgttgcgtc gaaacaggga
     2341 tagaaatttc aacgatcggg ttattgttct gttctgggat tgtcatgtca gcacctagtg
     2401 gtttgtcgtg gtgtcaattg tgctgtgatg catacaaagc tgccattgaa cgccattgtg
     2461 cgagcgacaa gacaaagact gaaccgcccc ggacctcttt tggctagcca gaaaaggcct
     2521 cgtttaacta aggctgtttc agacttacct taactattca ctactattca cttaggtaaa
     2581 aaaaataggt aatacagaag gttataaggt gaagggttgg catggcagta ttcatcgact
     2641 gttcataact gttcacatgg g
//
LOCUS       DAZFTN010000279         2598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFTN010000279 DAZFTN010000000
VERSION     DAZFTN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..230)
                     /locus_tag="ACHKV2_005257"
     CDS             complement(<1..230)
                     /locus_tag="ACHKV2_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO6942940.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDILKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGG"
     gene            complement(300..809)
                     /locus_tag="ACHKV2_005258"
     CDS             complement(300..809)
                     /locus_tag="ACHKV2_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO6942941.1"
                     /translation="MRGKLISAIHVAKRELALDDEAYTFALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMEMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            complement(806..1102)
                     /locus_tag="ACHKV2_005259"
     CDS             complement(806..1102)
                     /locus_tag="ACHKV2_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942942.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHVRWDEAEKAFHVRKKVNTV"
     gene            complement(1092..1289)
                     /locus_tag="ACHKV2_005260"
     CDS             complement(1092..1289)
                     /locus_tag="ACHKV2_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942943.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            complement(1282..1614)
                     /locus_tag="ACHKV2_005261"
     CDS             complement(1282..1614)
                     /locus_tag="ACHKV2_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942944.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(1653..1838)
                     /locus_tag="ACHKV2_005262"
     CDS             complement(1653..1838)
                     /locus_tag="ACHKV2_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942945.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            complement(1835..2386)
                     /locus_tag="ACHKV2_005263"
     CDS             complement(1835..2386)
                     /locus_tag="ACHKV2_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO6942946.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            complement(2390..>2598)
                     /locus_tag="ACHKV2_005264"
     CDS             complement(2390..>2598)
                     /locus_tag="ACHKV2_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942947.1"
                     /translation="EYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGV
                     KPANQKMTEILSDEFYAAEKRVRG"
BASE COUNT          666 a          685 c          557 g          690 t
ORIGIN      
        1 ccgcccatat aggctgcctg agccgcagcc accttgcgtg ccagtttacc ggccagtacc
       61 ggctcaatcc cctgacgctg aagttctgcg ccggtgactt caaccagttc tgacagggtc
      121 cgcggccagt tctttttgag aatatcatca gggatgtcat caaggcggtc gataagggcg
      181 tgcagttgct cactgtcacc accaaacatg ctcatctgag tttctgccat atcagcctcc
      241 ggttctcgcg ttcccggtaa gtttaaaata aaaatcccgc gttgtggcgg gatttggggt
      301 taacggggaa gtgatttttc atactgcctg caaacggcgt cataccccat cagcgtgcgt
      361 tcgggcattt ccatcgcggc cagcattttg cggcggtgcc atcgcttgag acgctccagc
      421 acatctgagg ccagggcggg gtgttgctcc agccactgcc agtttgccac accttcgccg
      481 ccgttctgcg cggctgtctg cgatttcacc cagcggttaa gggcggtttc cgcaccatca
      541 gagataaagc cctgccggtg catcaccttc cagatggcac gaattttggc ggtcaccgta
      601 cccggtttta aggcccggtt aaccggcttc tggcgcactt taaaaccgcg ttttttgaaa
      661 acatccagca cgcgggataa ctcatccggt gacatatccc ggcagctggt tttgccggtg
      721 gcggccagca gcgcgaatgt ataagcctca tcatccaggg caagctcgcg ctttgccaca
      781 tgaatggcgg atatcagttt tcctctcata ccgtatttac ctttttacgg acatggaaag
      841 ccttttctgc ctcatcccac cggacatggt gtgttggcgg aataatcatt atgtgccagc
      901 catctctggt tgttatccgg cagaaaaatt caccgctata taacacaatg gcctgctgaa
      961 tgagcgcatc agccgcccgt tcaaccggat gtcttttatc aaaaaagcag ataattttcc
     1021 ggcataacca ccgggtaata acaaatgcac cgaaaaagga tgtgatatat aagcaccaga
     1081 acaacagaga actattgtcc attatctttc tccttattca ctcttctgcg gcattcggca
     1141 cagtcatcag ggctttcaaa tgtatccggc tcgcgattat gagccagata taaccaccgt
     1201 ccacatatac tgatattttc gcctgtttta aaaaaatgat gcttcctggc cattagtggc
     1261 ctcgcccatc cagcgggtag actattcatt tgctttatcc tccatgctgg acagaatcat
     1321 atgtattaat gtcgggcgtg gagatatacg catttcatta agcgtctgaa gggcagcaac
     1381 agctgcctgt ttatcggtgc ctttggcaat ttccgcaaga gaatgcacaa tccataacgg
     1441 acttaacgcc acgactgaat ccagaatcat gtcatccata ataatttctg acacatcccg
     1501 cttgtcacat tccttatcca taaaagaaac gattttttcc acatcttccg gtttcatgcc
     1561 tccggcgata agaaatttgc gatactcata agacgatctt aattcaatac tcatattcat
     1621 ctcctgtaac tctgacaagg acgtgatttt tctcatttat gttttccagt aatacggata
     1681 tatatgtcga taagaagatt cataataaac aggaagagcg acatcctgat aaagaaccaa
     1741 atatctggca tcatggacag gtttcctgta atcgcaaata taaaaccaac ccccaacacc
     1801 ggaacgaaaa ttgcgaaaaa tacaagtctc tttatcatgg cgttaactcc cgtaatacag
     1861 cgtcctcggc agtccggcga tggtgataaa taaaattcac cgtactctcg gataattcaa
     1921 atttctcgcc tatttcccgg aaggtaagac gtcgtggtga ttcagcatcc cgtaattcac
     1981 gaataagatg aacatcatcg tcaggtatac gggtgaaggg cagcaattct ccgtattttt
     2041 tcatgcttac gccaatatat cgcgcccgat tgtggacaga atcccggtct cgccccagat
     2101 attctccgat ttgcttaccg gtcatcgttc tggcattttt tcggataaat ttatcttcat
     2161 catgtttaaa tcttggacgt ttgcatttga gcaactccgg gtaagtaccg cgtaataaaa
     2221 caatccggtt atgtacaccc cagaaacttc tttttaccct gacagcaata tccttaaccg
     2281 gggtggatgg ataaagcgca atcagcagcg catcttcttc aggtgtccac gcacggacat
     2341 gagcaggtgc ctgacctttt cgccccatgg gttgtaaaat catcatacat caccccctga
     2401 ctcgcttttc agcagcataa aactcatcag acagaatttc ggtcattttc tgatttgcgg
     2461 gttttacgcc gcactcaagg taaatcacat cgtcaacgca gaaccacttc acagggccaa
     2521 acatgattgc cgagaaatca atacccagcc agaacaacag tgcatcggtt ctggcatacg
     2581 tgacgggcga atattcac
//
LOCUS       DAZFTN010000280         2552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFTN010000280 DAZFTN010000000
VERSION     DAZFTN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..633
                     /locus_tag="ACHKV2_005265"
     CDS             <1..633
                     /locus_tag="ACHKV2_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6942948.1"
                     /translation="GVPGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADAL
                     GIDYTRANKGALSRAIRRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIG
                     NPRGLFKGGRRAFDDLSRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAI
                     AEKPGALRVLTHTLNQAWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            636..857
                     /locus_tag="ACHKV2_005266"
     CDS             636..857
                     /locus_tag="ACHKV2_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942949.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            870..1124
                     /locus_tag="ACHKV2_005267"
     CDS             870..1124
                     /locus_tag="ACHKV2_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942950.1"
                     /translation="MNIQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            1126..1407
                     /locus_tag="ACHKV2_005268"
     CDS             1126..1407
                     /locus_tag="ACHKV2_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO6942951.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            1404..1676
                     /locus_tag="ACHKV2_005269"
     CDS             1404..1676
                     /locus_tag="ACHKV2_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942952.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFTELVAMKASALMKDMDMSSGVIKGNEFNQQG"
     gene            1681..1974
                     /locus_tag="ACHKV2_005270"
     CDS             1681..1974
                     /locus_tag="ACHKV2_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942953.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            1986..2516
                     /locus_tag="ACHKV2_005271"
     CDS             1986..2516
                     /locus_tag="ACHKV2_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6942954.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
BASE COUNT          694 a          569 c          735 g          554 t
ORIGIN      
        1 ggcgtaccgg gtgtgggtaa aacggccact gcccgtaatt actgccagga gcaaccaaac
       61 acctggatga tcaccctgtc acccgcgcac tccagcgtca cggagtgtct gctggagctg
      121 gccgatgcgc tggggattga ttacacccgc gcgaacaaag gggcattatc ccgcgccatc
      181 cgccgtcgcc tgatgggaac gcgtggactg gtgattgtgg atgaggcgga tcatctgggt
      241 attgacggtc tggagcaact ccgggcaatt caggacgcca cggggatcgg gatggtgctt
      301 attggtaacc cgcgcggatt gtttaaaggt ggacgccgcg cctttgatga tttatcgcgc
      361 ctgttcagcc gtcttgcccg tacaaaacaa cttcgcaagg ccaaaaaggc ggatgtgctg
      421 gccattgcca gggcgtgggg gatcagtggt gaggccgagc tggccgtcat gcaggctatc
      481 gctgaaaagc cgggagcgtt acgcgttctg acacatacgc ttaaccaggc gtggctcacc
      541 gccagcggtg aaggcgcggc gctgacagaa aaacatatta atgcggcctt taaagaggtt
      601 tataccaacc ctgaattact ctcacaggtg tgattatggc ggtatttaat attcctgata
      661 tttacggacg cttttacctg gttaatttcg ataacgtgaa ggtgatttca ctggccgaaa
      721 ataaagaatg tggcgattta ctttttgaat ttaatgaccg cacacgaatg gtgatatctg
      781 ccggacttga tcgcgaaggt gcgacagacg tttacagcgg aatatgccgt tctgttggtg
      841 cgaaacaagt cagctaaatg aggtgttata tgaatattca atcctgcggt aacaaaatga
      901 atttattcga ctccctgaac agcgcgcgcc gtctgaccga acttgccggt gcggtactgg
      961 aacgcagtaa gcgctatccg caacgttttg cactgaaaac cacgccgccg gtaggcaacg
     1021 tgcagggaac cggtgaaatt gaaatcacca tacagaccaa cggcctgcgt cgccgtgtga
     1081 aggccacccg catcagcggc tgcacggttt actgggaggt atgaggtgaa aaaaaatctc
     1141 attgcatggg cgtggtcgag tggtcttatt gagtttggtt acgtcctgcc ggaaggtgca
     1201 ttaccgatag ttgccggaaa gcctgccacg gtacggcatg tgattgaggt tatggcgcgt
     1261 catggacgtg atgaacagga gcagttactg gttccgggga taccggaagc ggtgacggag
     1321 gaagaagcct ttaatgccat gattcggttc tgccgtgagg tcagacgccg ggtcagttat
     1381 ccaaacagaa cgaggaccag aggatgagta aagttgtacg cattattttc gaatacaagg
     1441 agcacgttat ccataaaaac gcggatggaa cagtgcgcat gggggtaagt ctggacatac
     1501 gttcaaccgg gataaagcag aaaggtgatg gacctgccat gatttttggg gtggttatgc
     1561 tcgcggaaag cagagacttt actgaacttg tggcaatgaa agccagtgcg ctcatgaaag
     1621 atatggacat gagttccggg gttattaaag gtaatgaatt taatcagcag gggtaattcc
     1681 atgagcaaag tacgcgttat ttttgaattt gaccacgtaa tgcatgaagt aaagccagcc
     1741 ggtaatgaca gtgaggaaat cacggagggg gttactgcca cggtaaaaat cgaacgtgat
     1801 acagagaaca gaccggcagg accctgtgat gtttatgcac aaattcttaa atatcacagc
     1861 cccacaatca ttcagtttct gacagatgaa ctacagggtt ccatgcaggc catgggggta
     1921 agcagctccg ttgaacgccg ttctgttcag aacggaccag atacattgca ataaggaaaa
     1981 acaaaatggc aaaacgcgtt acaaaattaa aggccgcagc agaggcggca ccgcagaccc
     2041 gcgaagaagt cagccgcgat atccgcaccc tgggcgatat tcagcgagag gcgctgcgcc
     2101 tggaaacagc gatgaatgac gaggtggcag aaatcaccgc ccgttatacg ccgcagattg
     2161 aaaaccttaa aaaagaaatc aaagtgcttt ttaaggggat tcaggactgg tgcaaaacca
     2221 accgcgatga gctgacgaac ggcggcaaaa caaaaactgc caatctgacc accggaacgg
     2281 tgtcatggcg gctgggaacg ccatcatgca gcgtcagccg tgatgtggaa ggtgtgattg
     2341 aaatgctgcg ccgtatgggt cttgagcgat tcatccgcac gaaagaggaa gtgaacaagg
     2401 aagccgtcct ggcggagccg gatgcggtga aagggattgc cggtattaag gtaaataaag
     2461 gcgctgaaag tttttatgtc gagccttttg aacaggacgc cggactgaac aaataacatc
     2521 gcattaaatc tttaaatatc acatcgtttt aa
//
LOCUS       DAZFTN010000281         2543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFTN010000281 DAZFTN010000000
VERSION     DAZFTN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            547..738
                     /locus_tag="ACHKV2_005272"
     CDS             547..738
                     /locus_tag="ACHKV2_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6942955.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(791..1024)
                     /locus_tag="ACHKV2_005273"
     CDS             complement(791..1024)
                     /locus_tag="ACHKV2_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942956.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1120..1743)
                     /locus_tag="ACHKV2_005274"
     CDS             complement(1120..1743)
                     /locus_tag="ACHKV2_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6942957.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1832..2341)
                     /locus_tag="ACHKV2_005275"
     CDS             complement(1832..2341)
                     /locus_tag="ACHKV2_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942958.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          746 a          648 c          530 g          619 t
ORIGIN      
        1 ctcctgacga tacctcccac catgttttgc gatggcacgc taccgccctt ccggtattta
       61 cccgcatcaa ccggtaccag aatgctgagt aacaaaccag ctagaattga cttaattttg
      121 atcattatag aataatgatc tgaactacag caacatgaca gatttatcga tcgatataac
      181 acaaatgaac agttctttat tctgacaaat atcgcaaaaa cagcacatta caaaatttat
      241 aaccataatt actggaaaag tgcagaaaat tattccgctg aatgctttta atacagttgg
      301 ttagcttaaa aaatagcaat ttttgatttc agtcattgaa agttgcaatg tttgacgatt
      361 caacagcatt taagtgagtt tcaggatatg ggaaggacat gaaaggagga gcgcaataaa
      421 atacacagga agaaggggaa aatgtttcgt aaaaacagaa aattaacagc gcacatcagg
      481 aaacgcagtc tcctgattca gtgctaccac atgaaaattt agttaactgt taccgtacta
      541 aaatttatgt cagaagatgt tccattaccg aaagtcaatc agcgatacaa agacgatcat
      601 ggtgcgcttg tcaccctgac cagtgttgag gaaacccggg tggtgtttat gcaggacggc
      661 tatccgcatc cctgcatgcg accgatgtac aactttctgg gaaaattcaa acccgaaccc
      721 cgggaagaaa cagaataaaa cgaaatccct gccggcaatc acactacctg atagcagcat
      781 aatccactta ttaattctca tccccggaga caagcccaga aaaccactcc gggtgttcac
      841 tgtatgccgt gttcagcgca tccctggaaa cgtttatata aaagctgtcc tgcaatttgc
      901 gggtaacaac ttctgccagg cgattccggt tatccaccag tccggcataa cctctctctg
      961 ccagccactc attcagttca tgttccagag acagattgat aatatcataa tcggaccttg
     1021 ccattcccgt ttccttagca catgttttca ggcacgggga taaagtataa agaatgtcat
     1081 actgctgctg ccagagcaat attccccctg atggttgtat cagagatggt atgccggtac
     1141 tttgcgggtt gttcccgtca ggttatcgtg cacctccacg gtcgtggtca ccaccggcat
     1201 tccggccttt ctcagcctca gaacatcagc cgcaatacgc tgactgccaa acctgaacag
     1261 gccgtccagt gcagtcacca gtaaacccgc ctccagtgca tacttcagcc gttcacgggg
     1321 cgctttcact tcccgggcaa cctgctgata tggcaatgat gtgttttcat tcccaaccac
     1381 gcgacgaata cggtgagaca gatgataccg gtatgtatcc ggcacaccag aaaggctggc
     1441 cttcaggctg tacacgcagc caaaccgttt atcgttaaac accacatttt tctggctgat
     1501 gccccattct tcacgcagcg cggcaatcaa ttgcggtgta cgggtaagca gcaagcgaaa
     1561 aggcagttca aaactggtga cgtaatccac attcagcagt gcaatacgca accgttcttc
     1621 tggcccggcc tctgtctgcc ggcactcctc cagaacatcc tgccactgcc ggcgaagacg
     1681 gggggattcg ttccgttctg taaaacagta tttatccgcc agatagtcaa ttcgtgtacg
     1741 catactgaaa ggaattccgt ataaagattc agcagacaaa actttatcag ccagtaaaaa
     1801 ttaacggaag cattgatatt cctcccgaca atcaccggat gattgttgca ataacctgcg
     1861 acataaagga ctgaacagcc gtttttaacg ccacgtattg ccctgatgaa ccaggccggg
     1921 ccacccgaaa acatctttcc acccggcatt gtccgccagc acttatcacc gaccaggtct
     1981 gttgcagtaa atccaccagt tgcgcacatt cttcatcagc catgacgccg actcgcggat
     2041 ggcaaatact cgtctggctg agcagcaaca gacatcgtat ctgtgctcct tcatgttgtt
     2101 cccgcaacca gcgtaatacc accggcgctg atgggctgac cgtgtgatta tgctccgtaa
     2161 tacgttcgtg tacccgtcgg tgaaaggaac tacaaaatgt ctgaatctgt tgaccataga
     2221 ttccttctgc cgaatagagc gtgaagtgaa atgccaacag atttggccag tggtccaccg
     2281 cctgctgaac aaaacgccgg atttcccccg gctctgaaat taaggcttcg gttatttgca
     2341 cgaaatgttc cttttcaacc cctgttgaat tttgtcaagc cggtgcagac acagtaaagc
     2401 gcgcatcaac acctgaaagg tacgatacaa acacaagtta caaacaataa gaacggaagg
     2461 acaattaatc agtgaggaat aataactaaa ttaaccaaaa gaatgttact gacaatacta
     2521 ccaatactta aatagcaata tat
//
LOCUS       DAZFTN010000282         2530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFTN010000282 DAZFTN010000000
VERSION     DAZFTN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1118)
                     /locus_tag="ACHKV2_005276"
                     /pseudo
     CDS             complement(1..1118)
                     /locus_tag="ACHKV2_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            complement(1359..2405)
                     /locus_tag="ACHKV2_005277"
     CDS             complement(1359..2405)
                     /locus_tag="ACHKV2_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6942959.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT          679 a          631 c          527 g          693 t
ORIGIN      
        1 gactttcagt atcatgaagt ttaacaacaa atcaattctg tttctgaatc caatccgcct
       61 gtcagaaagg taatgaatat aagccatcag gtcggttgtt ttttgtgagg aaaatcggta
      121 tttgtgagaa aaaagctcct ccggtcgaag attctgactc tgccattgct gcaggtattt
      181 atactcaata ttcagctcat taatttctgc ttttaacgaa gcgacctggt taatggcatt
      241 caggtgtttt ttcacttgtt gaagtacatc atcaatcgca tttgcagacg gtgagtcagg
      301 taagacttcc tcagaacgcg aaatcgacgt tgaaaaaaac tgttgcctgt tctccgtact
      361 ccccagtcgt gccatcacat atcccagttg ctgtttgtcc atcttttcat aaacattact
      421 gacagctgag ttattattgg acaaaattgc cactgtttta ttctgaatta atatattagc
      481 aacaatattg agtatagtct gtgttttacc tgttccaggt ggtccttcaa taactgaaat
      541 ctgtgaggaa aatgcccgtt cgacagccag caactgactt tcattaagac caaacggaaa
      601 aatcaaaccc catggcgaat cacgttttta ctctgtcctg aacagtatgc atgcaaggcg
      661 gtatccggat gaggaactat tttctttatc tgcctaagga tattctcatc gatatttctg
      721 ttattaccac gggcataaaa aatacgttca ttagccaccc tacacaagta attaaacacg
      781 gcatgctctg taaatgctga acactgttga aagtcaaggc cggtcgtttt catgatgtag
      841 ggtcgttcac cctcgcggta ataaacagca gcatatttat caccatatat aataaccagt
      901 tcagcccggg atgttacagc gttccctttc ctttcgagga aaacatttcg caaatttaca
      961 gttggcgtta tctgacatac agaaagcgga cgagtatatt ttctcccgct gcgaaaatga
     1021 caggtcagca taacctctct gtcgtcattt ccagcccaca gcgtccagtc ggtaatttta
     1081 tgagtcttat tttcaccgtc aacacaaatc gtgaccatat tcttactcag aaattatcat
     1141 ttaacttacg gaaaattgtt aaggcactcc cgatattagc tccggaggat tttccacaac
     1201 tccgggactg ttaacatatt tctgcctgcc atatatacat tgaatttagt cataacagtc
     1261 aataccagaa ttacaggaat gcgatgccga tacgctggtt attgaatact ggtagcccct
     1321 gagcactaat tcatcacgat actctgtatt tactgttttc actgcttttt acaaccattc
     1381 ccccaggtaa aatgctgctc ctccggtgat ggctgtgtca gaaactgcca tcccggaata
     1441 gccacgctga ccgccagcaa tgccgcacag acaccagcca caaacggttt cagaaacgtg
     1501 ggcggggatt tctgaaggga gggtgactgc tccgcctgca ctttcccctt gcgattatca
     1561 aggctcagtg catccagacg caccgcgcca tttgccgcaa gcagcccttc ccggttatcc
     1621 acgcttcccg gcagactcag cgaaaaatcc cctgttcccg tctgaatcag actgccttta
     1681 cgatttgaca ggctttgtgc cgatatctgc agtgtgtcgg catttatcgt accgccatta
     1741 ctgctgaact gcccggcagt ccgggcggaa agcgtatcgg cactcagttg cgcattctgt
     1801 gtagacagat ttccactggt tgcattcagc tcagtatgtt gtccctgtat ccggctgtta
     1861 cctaaatcca gcccctgctc aatgtcccgt aaatccagca gagaaccttc gtgtgccggg
     1921 aatgaggaat acaactgaat atagtcatca ctgtccggtg tcagacgcac atgtcgcaca
     1981 acaccgggaa taataaccag ccggaggata ccgcttttta aatcctgcga aggtgccaga
     2041 acacgggtgg tgacataacc atgatcgacc agacggttct gcaatgtact catcagcaga
     2101 ttaattcctt tcgcccccag gcaatgcccg accgccccgt tggcgatttt ttgtaaaggc
     2161 agccagtgtg gtaacgcatc agcccccttc agttccgtct gtttaatctg aaaacagggc
     2221 gtttcaacag gaaaattaat cttatgggca aaagagccag gtgcagaaag gcgaacatca
     2281 ggggctgaag gcgtcagttg ttgctcaagg gcttcctgac gctgctgctg aatgataaat
     2341 gcttcatctc cgctacgccg gacatccgct gcctgaagag ataaaggcga taataaagca
     2401 gacatcagtg tcagtgacag caaggacgga cagcgccgga ataagctttg ttcctcagtc
     2461 ctctcgttcc tgcaacataa ttttccagtc aaacgaaaaa ccttttattc atgtcatacc
     2521 aacaatcacc
//
LOCUS       DAZFTN010000283         2512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFTN010000283 DAZFTN010000000
VERSION     DAZFTN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..379
                     /gene="yiaW"
                     /locus_tag="ACHKV2_005278"
     CDS             56..379
                     /gene="yiaW"
                     /locus_tag="ACHKV2_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6942960.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFMLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            385..1521
                     /gene="yiaV"
                     /locus_tag="ACHKV2_005279"
     CDS             385..1521
                     /gene="yiaV"
                     /locus_tag="ACHKV2_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6942961.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1518..2492)
                     /gene="yiaU"
                     /locus_tag="ACHKV2_005280"
     CDS             complement(1518..2492)
                     /gene="yiaU"
                     /locus_tag="ACHKV2_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6942962.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          622 a          579 c          631 g          680 t
ORIGIN      
        1 catggcattt cataatatgc gttaattgtt cttttttata ttctctgagg gaaatatgtt
       61 cctggactat tttgcactgg gagtgcttat ttttgtattt ctggtgatat tctatgggat
      121 tattatttta catgatattc cctacctgat tgctaaaaaa cgtaatcatc ctcatgccga
      181 cgccattcat gttgctggtt gggtgagtct ttttatgctg catgttatct ggccattttt
      241 gtggatttgg gccacgcttt accgcccgga gcggggatgg ggaatgcaaa gccatgattc
      301 atccgttatg caactgcaac agcgcattgc cgggctggag aaacagctcg ccgacatcaa
      361 atcctcttct gccgagtaat acttatggat ttactgatta ttttgaccta tgtggctttt
      421 gcatgggcaa tgtttaagat cttcaaaatt cccgtaaata aatggaccat tcccacagcg
      481 gccctgggtg gaatatttat tgtcagcggt ctaattctgt taatgaacta taaccatccg
      541 tataccttta aagcgcaaaa agcggttatt tctattcctg ttgtcccaca ggtgacaggc
      601 gtggtgatag aagtgacgga taagaaaaat acgctgatta aaaaaggtga ggtgctattt
      661 cgactggacc cgacgcgtta tcaggcgcgg gtggatcggc tgatggcgga tatcgttacc
      721 gcagaacata aacagcgggc gttgggcgca gagttagatg agatggcggc gaatactcag
      781 aaggcaaagg ccacgcggga taaattcgct aaggagtacc agcgttacgc acgcggcagc
      841 caggcgaaag taaacccgtt ttcagaacgc gatattgatg tggcacggca aaattatctg
      901 gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa aacagatcca gagccagctg
      961 gatagcctgg tgttgggtga acattctcaa atcgccagcc tgaaagcgca gctcgcggaa
     1021 gcaaaatata accttgagca gacgatagtg cgtgcgccga gcgatggtta tgtgacccaa
     1081 gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc tacgtccggt gatggtgttt
     1141 atacccgatc agaaacgaca aatcgtggcg cagttccgtc agaactcctt gctgcgcctg
     1201 gctcccggcg atgatgcgga agtggtattt aacgctctgc caggcaaagt gttcagcggt
     1261 aagctggcag ccattagtcc agctgttccc ggcggagctt atcagtcgac cggcacctta
     1321 cagacgttaa acacagcgcc gggttcagat ggcgttatcg cgaccattga actgaatgag
     1381 cacactgatt tgagcgcttt accagacggt atttacgctc aggtggcggt ctactctgat
     1441 catttcagcc atgtctcggt gatgcgcaaa gtgttattac gcatgaccag ctgggtgcat
     1501 tacctttatc tcgatcatta atcttcgctt tccgccgctt gttcaggccg cagttgctgc
     1561 gactgagcaa gctggtggcg aatttcacgc agcatttcat ccagcacata ctggacccgc
     1621 cagggctggt attcccgttt acggaagata gccaccagat ccagccacca ttcatacggg
     1681 tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc tctgcacact tttgtctggc
     1741 gcaaacacaa tccccaggtg atttcgtgcc agctccagcg ccgattgcgt gttatcgcaa
     1801 acgtaatttc ccttcacgcg ataatcccgc acctctttac tcccggcgac gttgaagcgc
     1861 cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa tacaggagtg atgttcgagt
     1921 tcgtcaggac ggctaatagg atgtttctca agatagcgct ggctggcata agcggttacg
     1981 gcatattggg taataaaact ggcaaccagc gattcatctt ttggtggggc atagctgatt
     2041 aaaacatcac aatcatcagg aaattcgacg ccttcataaa aggcattacg ctcaagattg
     2101 caggttttta gcgataaggt gatatcaccg atatctttaa ttttatcgat tacatgtttg
     2161 gataaatagg taattatgcc ggttggggcg tagatggtga cccgaccacg tttctcatgc
     2221 ttataatctg caataaaatt attaagctgc tcgtttctgt ccagcatgtc gttgatgtac
     2281 ggtaatagcg cggtgccaaa aagtgtgagc atcagttgtc gggtggttcg gtcaaagact
     2341 tttaaaccca cttttgattc aaaatcagca agatatttgc tgacgttggc ctgtgcgatg
     2401 ccaagtacag tcgcggcatg gctgatattt tcactggcag cgattaccga gataattttt
     2461 aactcccggt acttaagttg taattttgtc atcgcaacag cccaattata ta
//
LOCUS       DAZFTN010000284         2502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFTN010000284 DAZFTN010000000
VERSION     DAZFTN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..1318
                     /locus_tag="ACHKV2_005281"
                     /pseudo
     CDS             90..1318
                     /locus_tag="ACHKV2_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1329..1484
                     /locus_tag="ACHKV2_005282"
                     /pseudo
     CDS             1329..1484
                     /locus_tag="ACHKV2_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
     gene            complement(1861..>2502)
                     /locus_tag="ACHKV2_005283"
     CDS             complement(1861..>2502)
                     /locus_tag="ACHKV2_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO6942963.1"
                     /translation="QVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYD
                     EDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTT
                     GIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREH
                     YLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          713 a          563 c          594 g          632 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataatgaaa aacataaaat
     1381 tttatctgca tggcaaagag ataccagcag aaagaatatt agatacacca gagtggaaag
     1441 actaccgtcc aaaatactcc ggttccacat ataaatattc ttaatgatag caaaaaatat
     1501 attttcgata taatcaatgt tatgattaaa gagtatttca tcagggcagg taaaaacaga
     1561 gtaaatcagc aaaagaagct gatcttcagc gatactgaca ctaactgacg gtttaagcgg
     1621 tcgtatgaag cagcagcttt ccgacggact gccatgcgga tcgtttacct tttgggctat
     1681 tccgcccgtc atcaagcggc tcacgagtac tgagtttatc aggatgatat tgctgacaat
     1741 ggtaattcgt tgaccgatgt gtacttcact acatatcggt caacaccgta ctgccgtgca
     1801 acatctccaa gatgtagcat cgtttttacc agaggaggca ggacatagat tgtaaaaaaa
     1861 ctatttcgat gcatttacca ttgagatatt ttccttcata ccaaaagttc tatcaattat
     1921 cgcaagtggt tcaacaggtg cggtcttatc tataacccaa ccactctctg taacacttcc
     1981 tcgtgtgata tattttgcct ttactttaac aactattaga taatgctcac gactaaaccg
     2041 atcagcaacg ctaagatccg atgtgtattc aggcaatatt cttccaatac gtacctgctg
     2101 ttgtgccata tattcatctg gtgcttttac atttggattt gaatcaggaa gccctccagc
     2161 agttttatat tcaacgatat tttcagcttg aataccggtt gtgccgcgac acaatatgat
     2221 gtctgaatcg gacagttcta tgtgcccctc cttaattttc gaataaattt gtttaatact
     2281 atgttctgga gttggtacta tttcagaaga taaagcaggc aacgtgtctt caagcaccgg
     2341 agaatcatcg tatatatcca acaaatcctc atcataccca atatctcctt ctcttaactg
     2401 agaatacggt ttatctatgt atctaataac tcccccacca tgaagacctt ctgatttata
     2461 aaatgtacca tcagcgtcac gaaagaaata aacattaacc tg
//
LOCUS       DAZFTN010000285         2490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFTN010000285 DAZFTN010000000
VERSION     DAZFTN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..172
                     /locus_tag="ACHKV2_005284"
     CDS             <1..172
                     /locus_tag="ACHKV2_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO6942964.1"
                     /translation="TNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSA
                     DEWLCRYDLPKV"
     gene            207..635
                     /locus_tag="ACHKV2_005285"
     CDS             207..635
                     /locus_tag="ACHKV2_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942965.1"
                     /translation="MCSTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            619..837
                     /locus_tag="ACHKV2_005286"
     CDS             619..837
                     /locus_tag="ACHKV2_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942966.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETRRQHEFTDMAGTPAVPAGKTK"
     gene            847..1074
                     /locus_tag="ACHKV2_005287"
     CDS             847..1074
                     /locus_tag="ACHKV2_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6942967.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRQAVDTPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARNVGQHR"
     gene            1055..1363
                     /locus_tag="ACHKV2_005288"
     CDS             1055..1363
                     /locus_tag="ACHKV2_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO6942968.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            1360..1650
                     /locus_tag="ACHKV2_005289"
     CDS             1360..1650
                     /locus_tag="ACHKV2_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO6942969.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRACVPGVKKPRARG"
     gene            1653..2234
                     /locus_tag="ACHKV2_005290"
     CDS             1653..2234
                     /locus_tag="ACHKV2_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO6942970.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            2234..>2490
                     /locus_tag="ACHKV2_005291"
     CDS             2234..>2490
                     /locus_tag="ACHKV2_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942971.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKP"
BASE COUNT          550 a          674 c          769 g          497 t
ORIGIN      
        1 aacgaacttt cgcaactcca cgcttctgcg cctgatcaat gccgggcaga tgcccgcgtc
       61 ctgtaagcag tatatccgct ggatttattc aaagggggtg aaactccccg gccttgaggc
      121 ccgccgttcg gcggatgaat ggttatgtcg ctacgacttg ccgaaagtct gaaccggctc
      181 tggcgaccgc tcatgataac gctgctgtgt gcagtactgc tgttacgggg tgtcctgtgg
      241 ctgcggtgaa taacgcctta ccggccctga ttgccggggc gtgcatggcg gcgctgggga
      301 ttatcgctgt actggttgcc gcgatatggg gaatgcacca gaagacgcag cgccttgaag
      361 acaataatca ggtgcttgtg cgtgagcggg atgaggcgcg tcaggtgctg gcaaatcagc
      421 agcataccct gcaactcatt tcacaaatca gtgaggcggc cacgaatgaa aagcaacaga
      481 acattcagca cagcgagggg cagcagagcg ttgtccgccg gtcgctggca gcagtgcctg
      541 cggcctctgc ccctgttcct gatgatgtgg ctgatcgggt gcgccgggcc gtctgtgaaa
      601 tacgtgcctg tgaagccggt gccgatcccc gctgaatggc tggctgactg cctggtccct
      661 cctgcgccgg agccgttcac gtttggggca tcggtcactt acaacctgca actgctggcg
      721 gtgatcaaga actgcaacgt ggacaaggcc agtattcgtc gtctggagac gcggcgacaa
      781 catgaattta ctgatatggc cggaacgcct gctgttccgg caggaaaaac gaagtaagga
      841 aaaggtatgg atgattcaga tcgtgctcag gctgtgatgg agcggggatc agaacgcgcc
      901 ctttgtaacc ggctgacacg taagcgccag gcagtggata cgccgggcag acgtgtctgc
      961 gcggactgtg gtggagagat ccccgccgca cgtcttgttg ctgtgcctga tgccatccgt
     1021 tgtgtgaact gccagaacat catggaggcc cgtaatgtgg gtcagcatcg ttaaagatta
     1081 tgttgtgccg atcctttcgg cgacggcaac cgctggcggg atattcatgg cgctgatgcg
     1141 caaaacgttt gtcccccgcg aggcctttga aaaactctcc gaccgcgttg agaaggtgga
     1201 aacccgcctg tcgtcactgc cgacagaagc cgaggtcaac cgtctgaatg tggaaatcgt
     1261 gaccctgcgg ggtgaactga aaaccacgaa cgccacgctc cgctctgtct cctatcagaa
     1321 cgaactgctg ctggagcagg ctgtaaggaa aaaaacgcaa tgagtgattt cattactgaa
     1381 gatcagcgtc tggtcattct gcggtcgctg gcagattaca acggtgaact gggtgaatcc
     1441 gtgctccagg actgtctgga tgattacggc caccgcgtgt cccgcgacac cgttcacacc
     1501 cacattgcct ggcttgccga gcagggactg gtgcgcaaac gcgttctgat taacggttat
     1561 ttcatcgcgg aactgaccgg tcgtgggcag gatgtggcgg aaggccgggc ctgcgttccg
     1621 ggtgtgaaga aaccacgcgc aagggggtaa cgatggacaa gccgacgcgt ggtcgtgtac
     1681 gcaaggtgga tttgctcccc gacagcatcc gtaagccgct gctggaaatg ctgcgtgaaa
     1741 aacgtctgac gcaggtccag atccgcgagg aaatcaaccg tctgatccga gaggcgggcc
     1801 tgccggaaga gcagcagctt tcaccggcgg ccatcagccg ggaagcctcc cgtaatgagc
     1861 tgattgcccg taacctgcgt gatttacgcg agcagacaaa agccatgatg gccgaactgg
     1921 gggacaagcc aaccggtgaa accacagcgc tgattctgga aatgtcccgc gccctgatgt
     1981 accgccgcct gcgtgccgcg accgaatctc tgaacagcga cagcgacgtg gatatgcgcc
     2041 tcatcaaaga catcctcctg tcagcacagc gtgcggaaag tgccgccgag cgcagcatta
     2101 agcgtgaaaa agaaatccgg gccgcatttg ctgaagagat ggcaaacgcg gtcaccgacg
     2161 agctgcgcgg cgtggacggg atgagtgaac agcttgagtc ccgtattaag gggattctgt
     2221 tgggtaaagc ctgatgaata acgccgaaga actctcaccc ttactgacga ataccgtctc
     2281 cacccgcaaa attgatctgg ccggtgaaaa agccctgctg ggcgtggatg tgccggacag
     2341 ccttgatttg cccggtgata tgccggtgtt tctggattat caggcccgct ggtttgagga
     2401 tgaaagcgag gtctgcatcg cggaaaaatc ccgccgcacc gggcttacct gggcggaagc
     2461 cgggcgtaac gtcattaccg ctgcaaaacc
//
LOCUS       DAZFTN010000286         2425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFTN010000286 DAZFTN010000000
VERSION     DAZFTN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(96..1508)
                     /gene="pykF"
                     /locus_tag="ACHKV2_005292"
     CDS             complement(96..1508)
                     /gene="pykF"
                     /locus_tag="ACHKV2_005292"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO6942972.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1516..1710)
                     /locus_tag="ACHKV2_005293"
     CDS             complement(1516..1710)
                     /locus_tag="ACHKV2_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942973.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2065..2274
                     /gene="fumD"
                     /locus_tag="ACHKV2_005294"
     CDS             2065..2274
                     /gene="fumD"
                     /locus_tag="ACHKV2_005294"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO6942974.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          636 a          522 c          583 g          684 t
ORIGIN      
        1 tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg cccatcaggg
       61 cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac agatgcggtg
      121 ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacaacgtc acctttgtgt
      181 gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc agtagaagtg
      241 atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg agccgttttt
      301 tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac tgcgcgagca
      361 gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc agcagtttca
      421 acggcaccac ggcataccgc ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg
      481 agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct
      541 tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc agtaccgtcg
      601 aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg
      661 gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca tttttcgatc
      721 atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag gtcgccacgc
      781 gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag gccttcctgg
      841 ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg
      901 atttcgataa cgtcagaacg cttacgaata aaggaagcag cgacaaagtc tacgccttgt
      961 tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag ggcaatggaa
     1021 acgccaggca ggttcacacc tttgttttca cccaggtcgc cgttgttcag cactttacag
     1081 ataactttgt taccttcaat ggcagtaact tccataccga tcagaccatc gtcaaccagt
     1141 acggtgttgc caacagacag gtcagttgtg aaaccttcat acgttaccgc aaccatttcg
     1201 ctgttgccga taacagattt atcagtagtg aaagtaaagg tctgaccagc tttcagagaa
     1261 acgtcgttac cgccttccag tttcatggtg cggatttccg gacctttggt atcaagcagg
     1321 atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat gcgctgaccg
     1381 tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc agcgtccagc
     1441 attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac aattttggtc
     1501 tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg ctttctgggc
     1561 gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc taaggatagg
     1621 tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct
     1681 gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga gtcaattggc
     1741 gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata
     1801 caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa aggttacgca
     1861 aaaacttcgc tgtttgcgca acgtctggtg ccggggttgt cactgtgaag ataagtgaaa
     1921 gttcatccgg tgtataaata catcatcgct caattgctac agttaaaata atttatcgcg
     1981 ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata tgctgatagt
     2041 tggcatgata aataggtata aagaatggga aatcggacaa aagaggatga actgtaccga
     2101 gaaatgtgca gagttgtcgg taaagttgtg ctggaaatgc gcgatctggg gcaggaacca
     2161 aagcatattg ttatcgcagg cgtattacgt acagcattag ccaataaacg cattcagcgc
     2221 agtgagctgg aaaagcaggc gatggaaacc gtcattaacg cactggtaaa atgaccattc
     2281 aggatgaggc gataagcttc atcctgatat tcttgcttca gttgcggtcc agttaatcat
     2341 tctaaataac aactgctgaa attatacgaa tttcgatatg tgttttaatt tgtaacgata
     2401 atattatttt tattttctgc aatga
//
LOCUS       DAZFTN010000287         2390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFTN010000287 DAZFTN010000000
VERSION     DAZFTN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..819
                     /locus_tag="ACHKV2_005295"
     CDS             join(122..371,371..819)
                     /locus_tag="ACHKV2_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFO6942975.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            836..1096
                     /locus_tag="ACHKV2_005296"
                     /pseudo
     CDS             836..1096
                     /locus_tag="ACHKV2_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(1238..2362)
                     /locus_tag="ACHKV2_005297"
     CDS             complement(1238..2362)
                     /locus_tag="ACHKV2_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO6942976.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          667 a          560 c          507 g          656 t
ORIGIN      
        1 taaaaatgca actataaaaa acagtcatca gtcggctgct tttcaatgtt taccttatca
       61 ttttctggta atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
      121 cgtggcttcc atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa
      181 cggcaaaagc actgccggac atcagcgcta tctctgctct ccttgccgta aaacatggca
      241 actacagttc acttacaccg cctctcagcc cggtaagcac cagaaaatca ttgatatggc
      301 catgaatggc gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt
      361 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca accgggcagt
      421 gatgtgattg tctgcgctga aatggacgaa cagtggggct acgtcggtgc taaatcacgt
      481 cagcgctggc tgttttacgc gtatgacagg atacggagga cggttgtggc gcacgtcttc
      541 ggtgaacgca ctctggccac actggagcgt cttctgagcc tgctgtcggc ctttgaggtc
      601 gtggtatgga tgacggatgg ctggccgctg tatgaatcac gcctgaaggg aaagctgcac
      661 gttatcagca agcgttacac tcagcgcatt gagcgacata atctgaatct gagacaacat
      721 ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct gcatgacaag
      781 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacctgagaa
      841 taatacctcc tgggtaagtg agcagatact ttcagcgcct ccgaaagatg taaatattcg
      901 acttggagtc ggttctttag aaggtacaac cgtctcacat gttcagcgat tgcatcattc
      961 gttaatcgca gctggtttgg aaagtaacct cactgtctat accggtggtc acgattatgc
     1021 ctggtggcgc ggagcaatta ttgatgaatt agcaaattat aattgcagga aggtatcaaa
     1081 taataactct gtgtaaaata tcgttttatc gtcagataac tctttctgca atattattat
     1141 gggagactat ctcatttatt tagtgatgtc cttcatttat gtgatgaagg acattatatt
     1201 actcataaca tttctcgttg aaaatttcag caatacgcta caattgtttc ccgttgtttt
     1261 ttaattttct catcaaaacc tctgcaatct ctgcaggact gggttgttca gccatttcag
     1321 cggctacctg atctgaacag aagcgtagtg aatcatcata aagaaggcga tttaccaaat
     1381 cactggtcag tccatgcttg tccggaataa tcccacaccc acgcttcgca acaatttctg
     1441 cattaacaga gcgatctgca ccttcgccaa acacaatctg tggtattcca ctatacagcg
     1501 ctgtcagggt attacctgcg ccaccgtgat gaataaatcc atcagctcca ttaaggaata
     1561 cacccatagg tatccagtca accagacgta catttgatgg tagttttcgt aaaccagtac
     1621 gagcatttat tgcaagttgc aaaatgatat cagcatcaac ttcatttgca gaatccataa
     1681 cccatgaaat cagctccaga ccatcaacca ttggttttac agtgccaagg ctgataagta
     1741 aacgtttccg atcagaatcc ctgtcccacc atgtttcctt tacagcacct ccgttataag
     1801 gaatatatct cattgagata accggttctc cagcattttt aagaatgctc atacttgggg
     1861 gagcaacatc aatccatgct aaatcacata gtggaccgct gactccatgc cgcctgtaag
     1921 cattgcttaa agaacggttt aacatctgaa tatgggcaga tgtatgcgcg aatccaacag
     1981 ccagcatcac tgaaggaatt ctatatttag cagcaaccaa tgggcctgcc ggatcaagag
     2041 ggggatagac tattaaatct ggcctccatt ttcctgcaaa atcgatgagg ttatctgcca
     2101 tttcatcgct aaaaaaagag aaatgaccaa taatattact ttttttcctc aactcttcct
     2161 gatggcgata tccagcctct gaatctaaac ctggaactgc atcaaaaaca accagtccag
     2221 cttctgctgc tttattcgcg aatttgccag cgctactaat tactacatca tgtccaatta
     2281 cacgaaaggc ctgagccaga gaaatcaatg ggtataacaa accgtacagc ggtgggccaa
     2341 taaaaagaat acgttttcgc attaaaaaag tccgtcttct gcttacctgt
//
LOCUS       DAZFTN010000288         2380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFTN010000288 DAZFTN010000000
VERSION     DAZFTN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..383
                     /locus_tag="ACHKV2_005298"
     CDS             9..383
                     /locus_tag="ACHKV2_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO6942977.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEQDAPRKKTGNKAEQARA"
     gene            380..799
                     /locus_tag="ACHKV2_005299"
     CDS             380..799
                     /locus_tag="ACHKV2_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO6942978.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            796..1356
                     /locus_tag="ACHKV2_005300"
     CDS             796..1356
                     /locus_tag="ACHKV2_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO6942979.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1357..1602
                     /locus_tag="ACHKV2_005301"
     CDS             1357..1602
                     /locus_tag="ACHKV2_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO6942980.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1599..>2380
                     /locus_tag="ACHKV2_005302"
     CDS             1599..>2380
                     /locus_tag="ACHKV2_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6942981.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWG"
BASE COUNT          528 a          635 c          727 g          490 t
ORIGIN      
        1 gggcaaatat gaatgaacat cacactgtgg cagcaacaga tgattcaggt cttcaggtca
       61 gtggtgacaa ttctgtcacg gtgctggcag aagtgcgttg cagtcgttcg gcgtttcgtc
      121 gtgcggggtt cctgttcacg cgtggacgtc agcaggttga ggtcaccccg gagcagcttg
      181 cccgactgga ggaggagcca tgcctcactg tgcgaatact acagacgtct gctgatgatg
      241 cggggagcgt ggcgggtgtg gttcatgcag tggccggtac agatttagcc gaagccgaag
      301 ccgaagccga agccgaagcc gaagccgaac aggatgctcc ccggaaaaag accggtaaca
      361 aagcggaaca ggcccgtgca tgaattacgc cactgaaacc gatatgcggg cgcgttaccg
      421 cgaggatttg ctcaggccgt tactggctgt gccccgttcg gatgagccgg acacgcgcaa
      481 gctgaaccgg gcgctgacag atgcgtcagc ccttatcgac agctatctgt ccgcccgtta
      541 cacgttgccg ctggaggtta ttccggccgt tcttgttcag cactgttgcg cgattgcctt
      601 ttattacctg tgcgatcagc gagcctccga tcaggcgcgt gaccgttacc gtgaagcgct
      661 ggcctggctg aaagatgtca tgaacggcaa tgtgccggtc ggtgtggata cgaacggtgc
      721 ggcccctgaa tccggggatt taccacaggt tcagtctgat gcagcggtat tcgggcgcaa
      781 ccagaagggc ttcatatgat tacggaaacc gaacaggcat acatcgcccg tatccgtgag
      841 tatttcggga atgaactggt gtctgttgac acgcatcccg gcgactggag cgacagcgta
      901 ctgcgcacca tgctgattaa cgccccggcg atctacgttg cctggctggg tgccggtgaa
      961 ggccgtaccc gtggtcgcct ggtcagtcac tgggtgttct acgtcatcgg cgacatgctc
     1021 aacgggcgtg aggccagccg tcccggactg tatcagattg tggcccggct gattgccgtg
     1081 cttaacggct tcagaacaga aaaaacctca ccgctttact ttgaaaaggc ggtcaacggt
     1141 tacaccgaaa cccaggcgga cagtggtgcg gtgatgtatg cgctttattt ctcctgcgag
     1201 gaaatgatcg caccgctgac cgataccagc tcgctggacg acttcctgcg tcattacgaa
     1261 acctttgtgg agccgcccgg tacgccgccg tttgaggcac atattgattt gccggggaac
     1321 acgacaacgg cggtcgaacc gccggaggaa ctgtaaatga aaaccatctt tatcaaaccc
     1381 gcgccgggac gcctgattcg tgatcccgac acaatgcgcc cgctggcaca ggagggggag
     1441 gaaaagccct ttacgccgtt ctggtgtcgg cgtcttgatg acggcgatgt cattcaggct
     1501 gaaaaagccg ctgaagaggc tccggctgta tctgcggatg caacgactgc gacagaaaaa
     1561 ccggtcgccc aaccggcatc cgataaggag aaaccgcaat gattaatttt gacgggatcg
     1621 gtaatgaccg ccgtataccg ctgattgagg ttgagtttaa taactccatg gctgtcaccg
     1681 gcactccggc acagcgtcag tgtgtgctgc tgtttggtca ggcgagaatg aaagagaatg
     1741 ccgtggacgg tgccggtgtt ctggatgttc cggtcaggat cacccgtgcg tctcaggcaa
     1801 cagaactctg ggggcgcggc tccatgattg ccctgatggt ggcggaattt atcgccatca
     1861 atcctgatgc tgaactgtat gccattgctc aggggaacgg caccggacag gcaacggctg
     1921 cggccatgaa tctgaccgga acggtcaccc gtgacgggat tgtttatgcc tgcgtggggg
     1981 ggcggcgtta tacactcccg gcaccaaaag gtaagaaagg caaagaactg accgacgagc
     2041 tggccgcgct gatcaatgcc gatcctgacg cgcccttcac ggcctcctct ggtgcaggaa
     2101 gtggtgataa tggcgcaggt ctgaaagggt ctctggggat caccgcccgc tttaccggtg
     2161 aatgctccgt tcatgatgtg cgcctgaact atcacgacgg ggaagcaacc ccggaaggaa
     2221 tacaggtcgc tatagcctat ccgaaacaaa aagcggctaa cccggatatc acccgaagcg
     2281 tggcgggtat gggcgatcgc cagtacaact atgtcgtcat gccctataag gatgatgcaa
     2341 acctgaaaat catcagtgat gagcttctga aacgctgggg
//
LOCUS       DAZFTN010000289         2378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFTN010000289 DAZFTN010000000
VERSION     DAZFTN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..430
                     /locus_tag="ACHKV2_005303"
     CDS             50..430
                     /locus_tag="ACHKV2_005303"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6942982.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            427..774
                     /gene="tnpB"
                     /locus_tag="ACHKV2_005304"
     CDS             427..774
                     /gene="tnpB"
                     /locus_tag="ACHKV2_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6942983.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            824..2362
                     /locus_tag="ACHKV2_005305"
     CDS             824..2362
                     /locus_tag="ACHKV2_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO6942984.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          552 a          657 c          693 g          476 t
ORIGIN      
        1 agacaaagtg gtgtccacca aataagtagt gggaaccaaa gtatcagata tgcagaaaaa
       61 tgtgactccc ggcaggcgaa aaggctgccc taattatcct cccgaattta aacagcagct
      121 cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa cttgctcttg aaaatggcat
      181 taacgccaat ctgttgttca aatggcgaca acaatggcgc gagggaaagc tgctattacc
      241 ttcttcagag agcccccagc tacttcctgt gactctcgat gcagctgccg aacagccaga
      301 atcgctcgca gaggacccgg aaaccctcag tatcagctgt gaggtaacgt tccggcacgg
      361 gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgctga tacaggaact
      421 gaagcgatga tcccgttacc ttccgggacc aaaatttggc tggttgccgg tatcaccgat
      481 atgagaaatg gcttcaacgg cctggctgcg aaagtacaaa cggcgctgaa agacgatccc
      541 atgtccggcc atgttttcat tttccggggc cgcagcggca gtcaggttaa actgctgtgg
      601 tccaccggtg acggactgtg cctcctgacc aaacggctgg agcgtgggcg cttcgcctgg
      661 ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc agctggcgat gctgctggaa
      721 ggtatcgact ggcgacagcc taagcggctg ctgacctccc tgaccatgct gtaaatctct
      781 ttatcctggt tgtcacagaa taagcccggt aaaatacggg cttatgaacg acatctcttc
      841 tgacgacatc ttcctgctga aacagcgcct ggccgaacag gaagcgctga tccacgccct
      901 gcaggaaaag ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa
      961 actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat
     1021 ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg ctgactggta gggtgtatga
     1081 cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact
     1141 accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc
     1201 actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg
     1261 ggttatccgg acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc
     1321 acctgcacct tcgcggccca tcgagcgggg tatcgccgga ccggggctgc tggcccgcgt
     1381 gctgacctcg aagtatgcag agcacacccc gctgtatcgc cagtcagaaa tatacggccg
     1441 gcaaggtgtg gagctgaggc gttcactgct gtcgggctgg gtggatgcat gctgccggct
     1501 gctgtctccg ctggaagagg cgcttcatgg ctatgtcatg actgacggca aactccatgc
     1561 cgatgatacc ccggtccagg tactgctgcc gggtaataag aagacgaaga ccgggcggtt
     1621 gtgggcgtat gttcgtgatg accgcaatgc agggtcagcg ttggcacctg cagtgtggtt
     1681 cgcttacagc ccggacagaa aaggcatcca tccgcagact catcttgcct gcttcagcgg
     1741 tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg tatcgcaatg gtgggataac
     1801 ggaagctgcc tgctgggctc atgcccgccg aaagatccac gatgtgcacg tccgcatccc
     1861 gtcagcactg acggaagaag ccctggagca gatcggtcag ttgtacgcca tagaggcgga
     1921 tataagggga atgccggcag agcagcggct tgctgaacgt cagcgaaaaa cgaaaccgtt
     1981 gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag accctgtcgc gacactcaga
     2041 gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa
     2101 cgatggctgg gtggaaatcg acaacaacat cgctgaaaat gccctgcggg cggtcagtct
     2161 gggtcgtaaa aacttcctgt tcttcggctc tgatcatggt ggtgagcggg gagcgctact
     2221 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     2281 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     2341 catagcactg ccagctgaat aacacatccc cgtcaata
//
LOCUS       DAZFTN010000290         2376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFTN010000290 DAZFTN010000000
VERSION     DAZFTN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(596..1495)
                     /locus_tag="ACHKV2_005306"
     CDS             complement(596..1495)
                     /locus_tag="ACHKV2_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6942985.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1594..2316
                     /locus_tag="ACHKV2_005307"
     CDS             1594..2316
                     /locus_tag="ACHKV2_005307"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6942986.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT          671 a          531 c          540 g          634 t
ORIGIN      
        1 acgaaatgat ttttacaaca taccattatc gctaaacaaa atcgcaccca tcaaatcaac
       61 ttacgttaca atcactacaa agcgattgca tttaaattca aaacagtttt ctgttgggcg
      121 cattttttgt tgtactggac taaagagaaa aaatggggta aggaacaaat accttccgtg
      181 ctcaaaaatt aggtttttat gcacttatta atcagtcaag gtgaacagta aagtgttcac
      241 ttcttatcaa tcgttaattt catatctttt tgaatattaa tgaaaaaaaa cacaatgagt
      301 agtatgaaca gttttccccc aaaaaaagtt ttttctgcct tgagaatgcg cggaaagcga
      361 acgccttcac caatgtgtat aggggtgtgt gtagtttaaa tcttctattt taatttatgt
      421 attaaaatca ttgtgttggt ggcgattatt ggttcctatt atcgcaccat ttaaatcaag
      481 tagttacccc atatttaaat acaccacgtt tcctcctgtg ccgcatttgt gccattgtaa
      541 ccctggcaat taatcaaaat actgttctga catcaggcaa tgcaggtgca gacatttaag
      601 ccaattgctg ccgccattcc ttaacgtggt caatcagagc gcggagcttt ggtgcaatat
      661 tgcgacgctg tgggaaatac agatagaagc ccggaaattg tggaagaaag tcatcaagca
      721 gagatacaag cttaccgctt tcaatatacg gcctgaaagt ttcctgagtg gcaattgtta
      781 ttcctccgcc ggcaagagcc agcctcaaca tcagacgcag atcattagtc gtaatctgcg
      841 gttcaatcgc aaggtcgaaa gctctcccgt tttcttcaaa tggccagcga taaggcgcaa
      901 cctccgggga ctgacgccag ccgatacact tatgattcac aagttcacgc ggatgaacag
      961 gagtgctgtt ggctgcgaga taggagggag aggcaacgac catttcacgc tgcctgccgg
     1021 taaggggcac agcaatcatg tctttttcaa tgacctctcc gagccttacg ccagcatcgt
     1081 aacctgcggc cacgatatcg aattcctcat ccgtgacaac aatatcaagc gtaacggctg
     1141 gattggctgc tgcaaacgaa gcgattagcg gacctgaaag aaattcttca gctattgacg
     1201 tcaccgcgat tctgagaagt ccgcgtggca catggtcaga aaccatctct tcgaacgctg
     1261 cctcaatacc ggataaaggt aatgacagcg atttatgcag ccgttctccc gcttctgtca
     1321 aattaactga gcgcgtggtc cgcattacaa gcattgtccc gaatgcatct tcaaggcgtc
     1381 ttattccctg actgaccgct gaacgggtaa cgcccagccg tgcggcggct ttactaaaat
     1441 tactctcttc tgctaccgcg ataaaaatgg gtaaaagatt aagatcgatt ttcattgttt
     1501 aatgccgcta accactgagt acattgaaag caggatactg gaaccaatca taaagttcta
     1561 ttctctctcc gaacacattt gaaggagaac gtaatggata aggtcatatt aattaccggc
     1621 gcatcaagtg gtattgggga aggtattgcc agagagcttg gaatgacagg tgcaaaggtt
     1681 ttactgggag cacgcagagt tgagcgtatc gaagccattg caaccgaaat ctgccgcgca
     1741 ggaggaattg ctaaagcgcg ggagttggat gtcacagacc gacagtccat ggccgatttc
     1801 gtgcaagcag cgctggatag ctgggggcga gttgatgttc ttatcaataa tgcgggcgtt
     1861 atgccgcttt caccgcttgc agcaggaaaa caagatgaat gggcgctcac tattgacgtg
     1921 aatatcaaag gtgtactgtg gggaattggc gctgtacttc cggtgatgga agcacagggt
     1981 tccggtcaga taatcaatct tggttctatt ggtgcccttt ctgttgtgcc cacaggcgca
     2041 gtctattgtg catcaaaatt tgcagtacgg gctatttctg atggcttacg gcaggaaagc
     2101 tcgaaaatcc gtgtgacctg cgtaaatcct ggcgttgtcg aaagtgaact ggcttcaacc
     2161 attacccatg tggaaaccat gaaggcaatg gacgcctacc gttctgttgc acttcaaccc
     2221 gcggatattg cccgtgctgt aaggcatatt attgaatctc ccgaaagcgt tgatacaacc
     2281 gaaattacca tcagacccac tgcatctgca aactgaagaa taacttttac attcacgttt
     2341 tattaaatag gtcagaagga atatttatat atccct
//
LOCUS       DAZFTN010000291         2374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFTN010000291 DAZFTN010000000
VERSION     DAZFTN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            274..468
                     /locus_tag="ACHKV2_005308"
     CDS             274..468
                     /locus_tag="ACHKV2_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942987.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGGWL"
     gene            465..2354
                     /locus_tag="ACHKV2_005309"
     CDS             465..2354
                     /locus_tag="ACHKV2_005309"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6942988.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNVSE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITSMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYSTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGITLFDKKHSNLIDAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT          695 a          554 c          603 g          522 t
ORIGIN      
        1 atcactggaa cgttcccgaa aacatttaca aaaaacaggc gcaaaaaaag cgcccccgat
       61 tgctgttacc ggaggcgctt ttacacgaca aaggagtttt tatcgccagg atgacgagtc
      121 ttaatactgc ttcaatggca aaaatgcgtc aataactttg cctttctgag aataatcaga
      181 aaaatcataa tctgattttc aggtagaaaa tgatttatct attactttta tcgatcaata
      241 atgatgcccg ttaatcaaaa cggaggcgga tttatgccag agaacaacac cagaaagccg
      301 gataaaagtg ccacggtaca catagacgcc ggaactatgg agaagatcga acgctatcag
      361 cagttcatca aagataatca cccgggtatg ccagtaccca cgaaaggaca aatcacacgc
      421 agcgcggttg aatactggta cagggcaacg ttagggggct ggctatgaaa acatggtttt
      481 ccattaaggc tatggcagat gttgtctatg tgcgcattta tgacgagatc ggcgggtacg
      541 gtgtaaaagc atcggcactt actgacgaga tcaacgcgtg tggtaatgtg tctgaaatcc
      601 atcttcgcat ccattcacct ggtggcgaca tctttgaagg gctggctatc tataacgccc
      661 tgaaaaatca tccggcaaag aaaattgtac acattgaagg catggcggct tctatggcct
      721 cgtttattgc catgtgtggc gatcacatcg ttatgcctga aaacgcgatg atgatgatac
      781 atgcgccccg tggtgttact gccggagtgt cgggcgacgt tcgccgcttt gctgacctga
      841 tggacaagct gggcgacacg atggcggaaa cctacgccgg aagaacgggc aggagcaaac
      901 aggagatcac ctcaatgatg gaggcggaaa cctggatgga tggcaatgag tgtaaggcga
      961 acggcttcgc agatgaggtt atacccgcga ttacagcaat ggcccgaatt gaatcaaaac
     1021 gaatcggaga tttttcaaat atgccggaaa aaattaaaag catgatcagc cagaaaactg
     1081 gcagtggcga acaggaacga cttaacggaa tccgtgaatt gtttggcacc ttcaacggaa
     1141 gatataacga cctggctata agttgtcttg cggattcaga atgtagcgtt gagaatgcac
     1201 gcgaacgcct tttactcgct atgggtaaag aatcaacgcc aacaaacaaa accacccccg
     1261 caaatcttta ctacgcgtac acggataacg gcaatataac cggcgatgcc atgcgtcagg
     1321 ggcttaatgc gcgtcttggt cacgaacggg ccgaacgcgg taatccttac gccatgatga
     1381 gccttttcga tatggcacag gcatcattaa cccatcgtgg tataagcacg ggcagctaca
     1441 gcacacgctc gcagatagta aacgcggcat tcacccacag cagcagcgat tttaccgata
     1501 tccttgctgg tggcgctgaa aaatcagtgc ttgcaggctg ggagcacagc ggcgaaacat
     1561 tccgccagtg gacgaaaaaa ggttcccttt caaacttccg ggaagcccgc cgcgttggta
     1621 tgaatggctt ctcaacgtta aacaaagtgc cggaaggggc agaatataaa tacatcacca
     1681 ccagcgatcg cggtgaaccc atcgcgctgg ctacttacgg gaatattttc agcattaccc
     1741 gccaggcgat aatcaatgat gaccttgatc agttatcaac ggtgccaatg gctatgggcc
     1801 gtgcagcatc aagaacggtg ggaaatctgg ttaatctggt gcttacaggc aacgtaaaac
     1861 tttctgacgg aataacgctg tttgacaaaa aacacagcaa cctgattgac gcaggactga
     1921 caacaccggg acttagtgca gcacgtcacc tgatgcgcac acagaaggac aaaaatggcg
     1981 aagtgctgaa tattgcgcct aaattccttt tagttccggc agcactggaa gatcgcgcgt
     2041 tgcagatgat taactcaacc gcacctttcg gggctgataa aaacagcggg atctttaacc
     2101 cgtatcacaa gctacttgat atcatcgtcg atccccgcct tgatgatatc agcgaaaaac
     2161 aatggtacat gctttccgca cagggaacgg acacaatcga ggtggcttat cttgatggca
     2221 atgacgagcc ttaccttgaa cagcaggaag gttttatcgt tgacggcgtg gcctggaaag
     2281 tccgtattga tgcaggtgtg gcagctctgg attatcgcgg tatggtcaaa tcaggcggga
     2341 cagattcact atgacaacaa ggcggcacca gccg
//
LOCUS       DAZFTN010000292         2306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFTN010000292 DAZFTN010000000
VERSION     DAZFTN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..1504
                     /locus_tag="ACHKV2_005310"
     CDS             146..1504
                     /locus_tag="ACHKV2_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6942989.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1602..>2306
                     /locus_tag="ACHKV2_005311"
     CDS             1602..>2306
                     /locus_tag="ACHKV2_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFO6942990.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGGLLSFAMSVFQMNRDTTITAGILLKQLENVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDF"
BASE COUNT          688 a          370 c          490 g          758 t
ORIGIN      
        1 taaagcactt aggcaataat aattacattc agcaactatc atcatcggta ttgtttgtgg
       61 gcggaatttc atattgacaa cagtacagtt cttatatcta ttaataatag aaagggatct
      121 acaacctaca gattggtgta gctttatgga aaaagactat ttgagaatta gtagtactgt
      181 attagtgagc ttattgtttg ggcttgcttt ggttttggtg aatagttggt ttaatcagcc
      241 aggcgttgag gaagttgttc cacgatcaac ctatctgatg gtaatgattg ctttattttt
      301 tatcgatact gttgcattta tttttatgca gttgtatttc atttatgacc gtaggcaatt
      361 ttcaaactgt atacttagtt tggcttttct gagttgtttg atttactttg ttaaaaccgt
      421 cattatcatt cagcaaatta ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat
      481 tgcaatctat tatttgtttc gtcagatgag tttgtgcata ttaatatttc tggcattggt
      541 gaataaagtt agtgaaaaca caaaacagcg caatttattt tccaaaaaaa tgactttgtg
      601 cataagtttg ttttttgttt ttgggggggc gatagttgct cacatacttt ctagtcatta
      661 tgagagctac aatttacaca ttgccgagtt aaccaatgaa aatggtcagg tggtttggaa
      721 agcctcatat gttaccataa tgattttcat gtggttaacc ttgctttcgg ttaatctata
      781 ctttaatggt ttacgctatg atatctggaa tggagtgacg gtgattgctt tttgtgccgt
      841 actgtacaat atctctctgt tatttatgag taggtatagc gtgtccactt ggtatattag
      901 tcgcactatt gaagtagtca gtaagttaac cgttatggta atatttatgt gccatatatt
      961 cagtgcgcta cgagtaacaa agaacattgc acatcgcgat cccttaacca atatatttaa
     1021 cagaaattat ttttttaatg aactgacagt tcaatcagca tcagccaaaa aaacgcctta
     1081 ttgcgtcatg attatggata tcgaccactt caaaaaagtc aacgacacct gggggcatcc
     1141 ggttggcgat caggtgataa aaacagtggt gaatatcatt gggaaaagca tacgaccaga
     1201 tgacctttta gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg acatcgatac
     1261 tgaacgtgcg aaagctttag cggaaaggat tcgggaaaat gttgagcgtt taactggcga
     1321 taatcctgaa tacgctatac cacaaaaagt gacgattagt attggcgctg ttgtgactca
     1381 gggaaatgcg ttaaacccga atgagatcta tcgactggct gataacgcac tatacgaggc
     1441 gaaagagacc gggcgtaata aggtggttgt gagggatgtg gtgaattttt gtgagtcacc
     1501 ataaagcggc attttagcgg gtgattttgg taagcgctaa atagctattc gcagacatgc
     1561 gtaacaattt tcagaaatat catagtacta tatgatctta aatgcttatc attgatcagt
     1621 ctgctatcac ccgcttttac tggcaattgt actataattt cttaggttgc atcaccctga
     1681 tgcgtgggga aacatgtatg gtattcaaca aaaaaatgtt tgtactcatt ataataccag
     1741 gcattctggg ggggttactg tcgtttgcta tgtcagtatt tcaaatgaat cgagatacga
     1801 caattaccgc cggtattctg ttaaaacagc ttgaaaatgt tacgcaaatt gtcaaacata
     1861 cgacaaagct tacttctatt ctggtgatga aaccctgcaa agatatcctg gaacaattga
     1921 ttgctaacgg agcattgacc ccctatgttc gaacaacggg tcttatagaa aataatttcc
     1981 aaatatgttc ttcagttagc ggtttcaaaa aaatgaatgt taacgatgtt tacggtacga
     2041 gctttcacaa taaaaataaa gaatcacgca tagtatcaat tagcggtaca agttttgtac
     2101 caggtaaaac agccatagtg ttccttatgc caatagggaa tgatatgaca gcttttagta
     2161 ttgtagagtc acgatatatc tatgatctga tggatgttct tgatgatgag aatgatgatt
     2221 cattttctct tcgctttact gaagggcctg caattattag tggtgtaaat aataatgaca
     2281 ggttatatat gttaaaaaaa gatttt
//
LOCUS       DAZFTN010000293         2272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFTN010000293 DAZFTN010000000
VERSION     DAZFTN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            181..387
                     /locus_tag="ACHKV2_005312"
     CDS             181..387
                     /locus_tag="ACHKV2_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6942991.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(643..915)
                     /locus_tag="ACHKV2_005313"
     CDS             complement(643..915)
                     /locus_tag="ACHKV2_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942992.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1075..1608
                     /locus_tag="ACHKV2_005314"
     CDS             1075..1608
                     /locus_tag="ACHKV2_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6942993.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1829..1942
                     /locus_tag="ACHKV2_005315"
     CDS             1829..1942
                     /locus_tag="ACHKV2_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942994.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1944..2207
                     /locus_tag="ACHKV2_005316"
                     /pseudo
     CDS             1944..2207
                     /locus_tag="ACHKV2_005316"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2164..>2272
                     /locus_tag="ACHKV2_005317"
     CDS             2164..>2272
                     /locus_tag="ACHKV2_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6942995.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAP"
BASE COUNT          584 a          461 c          622 g          605 t
ORIGIN      
        1 aagatattgt gtcatttata acctgaatca ggggaggccg gaatgttatc tggcattttt
       61 agcagagcct gaatgccata atcacggctc ccggagttgg ccgtcagtgg gtgacactgg
      121 cggctttttt gtttttcttt actttcattt tctgtcggcg gtgacggaga catacatcag
      181 atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtggggac ggaatactgg
      241 ttactgcagc tgctggacaa agtctctccg tcccagtggg tggcaatagg tgtgctggga
      301 agtctgctgt ttggcctgct gacgtatctg actaacctgt atttcaaaat cagagaggac
      361 cgtcgtaagg tggcgcgggg agagtagtcg atgaataaac aatacgaact ggttgtaaaa
      421 tgaatatttc taactgaaaa aacgttccat gaggtaagaa aaggtcacag gcaatcaata
      481 acaggacgtg atgaaagacc cttgcatttg tgcgctttct ctttagatag cagcagatac
      541 tgaaaatctg agttgtcggg gagtcaggga tacagctgtg caagagttgg tcattgtgat
      601 tccattgaaa tcctgtatgc catgaagggc aggattttat ggctacctga gctttggtga
      661 tagtaagttg aaaattcgca ttttttgctg acatgcgtaa cgagaatccc ataagcaggg
      721 aggacttaat tcttcattaa cccatgcgtt gatattatgt ttcagccgtt gaagcatcag
      781 cggtgttaat gttgtggtaa taatatccag cgttttatgt gagatcttac cgtaagggtc
      841 tgcaagaatg ctgcttgttg cttcgttatt atctgccatc agaagaagta actctgattt
      901 aacgttttct gtcattagtt gtaaaaatct tctgcgcaaa ctttctttac tgttcattta
      961 tatggcttca tttgttgtaa tctgctgcgt ctcaagggat atgtttatga gagcgaccat
     1021 gagtgttgga ttatatacct aacatatcaa gggattagaa atcgataaat ccccatgaac
     1081 gaaaaaataa aatacggcct gtcggctgcc gttctggcgc tgattggtgc aggtgcttct
     1141 gcgcctgaaa tcctcgacca gtttctggat gaaaaggaag gtaaccacac cacggcatac
     1201 cgtgatggtg cggggatctg gaccatctgc cgtggtgcca ttctggtgga tggtaagcct
     1261 gttattcctg gcatgaagct gtcaaaggaa aaatgcgacc gggttaatgc catcgaacgt
     1321 gacaaggcgc tggcatgggt ggagaaaaac atccgggtgc cgctgaccga accccagaaa
     1381 gcggggatcg cgtcattctg tccgtacaac attggccccg gtaagtgctt cccgtcgacg
     1441 ttttataaac gaattaatgc aggcgatcga aaaggtgcct gtgaggcgat tcgctggtgg
     1501 attaaggacg gtggcagaga ctgccgtatc cgttcaaata attgctacgg tcaggtctca
     1561 cggcgtgacc aggagagcgc gctggcgtgc tgggacatcg acagatagca gaatattttc
     1621 ctgaaaaatg acgttggcca acgcgggtgg ataacacgaa atcctgaaaa ctggtaaaac
     1681 ctaagtgaat aaaagtaaaa accccgtttg ttggcagcaa gcggggtttt gtgttttctg
     1741 accttgagta aggcaaggga gaaattatgg gtagggaggt actttccctg tgaggaagta
     1801 taaaagattc tttctgaggt tgtccattat gaaaggcatt gaagtggaga cgccagccag
     1861 tctggattta acaagagcgg cagcttttgc cattcgtatt gtggccattg ctgttctggt
     1921 ctgggcaatc cgttggtggt gatatgaacc gtgttctgtg tgtggttatc attgtcctgc
     1981 tggtggcctg tggtgcgctt agtctggggc tgaatcatta ccgtgatcac gccatcatct
     2041 acaaagagca gcgcgataaa aaagccagag agctggaaca ggcgaatgcc accattactg
     2101 acatgcaggt gcgccagcgt gatgttgctg cgctcgatgc aaaatactcg agggaattag
     2161 ccgatgcgag agctgaaaat gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc
     2221 tgcggatcaa cgccacctgc cccggtaccg tgcgtgaagc caccggcacc tc
//
LOCUS       DAZFTN010000294         2265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFTN010000294 DAZFTN010000000
VERSION     DAZFTN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..633
                     /locus_tag="ACHKV2_005318"
     CDS             <1..633
                     /locus_tag="ACHKV2_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6942996.1"
                     /translation="LWEIDLTVQGGERYFFCNELNEKGEAVTWQGREYQAYPIDGSGF
                     EMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPE
                     ADPEQELSDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDEC
                     GYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            644..1387
                     /locus_tag="ACHKV2_005319"
     CDS             644..1387
                     /locus_tag="ACHKV2_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6942997.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            1384..1962
                     /locus_tag="ACHKV2_005320"
     CDS             1384..1962
                     /locus_tag="ACHKV2_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6942998.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          478 a          551 c          762 g          474 t
ORIGIN      
        1 ctctgggaaa tcgacctgac ggtgcagggt ggtgagcggt attttttctg caatgagctg
       61 aatgaaaaag gggaggcggt tacctggcag gggcgggaat atcaggcata cccgattgac
      121 ggcagcggtt ttgagatgaa cgggaagggc agcagtgccc gcccgtcgct gacggtgtcg
      181 aatctgttcg gtctggtcac cggaatggcg gaggacctgc agagcctggt gggggccacg
      241 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaattttgt ggcgggcaat
      301 ccggaagcgg acccggagca ggagctgagc gaccgctggg tggtggagca gatgtcagag
      361 ctgacggcca tgacagcctc gtttgtgctg gcaacaccga cggagacgga cggagcgctg
      421 tttcccggtc gcattatgct ggcgaatacc tgtatgtggg attaccgggg agatgaatgc
      481 gggtataacg gtcctgcggt ggcggatgag ttcgacaacc ccaccacgga tatccgtaag
      541 gacagatgca gcaagtgcat gcgcgggtgt gagatgcgcg gcatggtggc taattttggc
      601 ggtttccttt ccatcaataa actttcgcag taaatcctgt tttatgacac agactgaatc
      661 agcgattctg gtgcatgccc ggcggtgtgc gcctgcggag tcgtgcggct tcgtgatagg
      721 caccccggag ggcgaacggt accagccctg cgtgaatatc tccgcagagc cggaggcgta
      781 ttttcgtatt gcgccggaag actggctgca ggcagagatg cagggggaga ttgtggcgct
      841 ggttcacagc caccctggtg gtctgccctg gctgagcgag gccgaccggc ggctgcagat
      901 aaagagtgcc ctgccctggt ggctggtctg ccggggggaa attcataaat tccgctgtgt
      961 gccgcacctg accgggcgtc gttttgagca cggggtgacg gactgttaca ccctgttccg
     1021 ggatgcatac catctggcgg ggataacgct gccggatttt gagcgtgagg atgactggtg
     1081 gcgcaacggt cagaaccttt acctggacaa tatggcggcg actggttttt accgggtgcc
     1141 cctgtcctct gcacaggcgg gcgatatcct gctgtgctgc tttggcgcat cggtggccaa
     1201 tcatgccgcc atatactgcg gcaacggtga actgcttcac catctgcctg aacaactgag
     1261 taaacgggag aggtattctg aaaaatggca acgacgaacg cattctgtct ggcgtcaccg
     1321 ccactggcac gcatctgcct tcacggggat ttgcaacgat ttggccgccg cctcagcctg
     1381 tacgtgaaca cggcagcgga agccatccgt gccctgtcgc tgcagatgcc gggattccgc
     1441 cgtcagatga acgaaggctg gtaccagata cgtattcgcg gtgaggacac ggcaccggag
     1501 gcggtgtacg cccgtcttca cgaacctctg ggtgaggggg cggtcatcca tattgtgccg
     1561 cgactggccg gggccggaaa gggtggactg cagattgtgc tgggggcggc agccatcgtg
     1621 ggctctttct tcacggccgg ggcatcaatg gcgttatggg gttcagccct ggcagccggt
     1681 ggtttttctg ccaccacgat gctgttctca ctgggtgcca gcatgatact gggcggtgtg
     1741 gcccagatgc tggcaccgaa ggcaaaaaca ccggagtaca gggcgacgga taacggtaaa
     1801 cagaacacgt atttttcgtc actggataac atgattgccc aggggaaccc gatgccggtg
     1861 ccttatggtg aaatgctggt tggttcacga cggatatccc aggacatcag cacccgtgat
     1921 gagggcggag acgggaaagt ggtggttatc gggcggggat gaaaataaaa aaatcccgca
     1981 gagttagcgg agctgcggga gagaacgatg aagattaacg ttatggagtt atttttcagg
     2041 catcaaaaaa gtaatgcagc gtcattattg cggctacagg caattgccgg aaatgtgaag
     2101 agtttcagaa attttattcc gtcatgacac aggcaccctc cggggtgcct gttgttttct
     2161 ggcataaaca gattcagaca tcagacagga gagggggaca gagtgggtaa agggggcggc
     2221 aaggggcaca cgccggtaga ggcaaaggac aatcttaagt ccacg
//
LOCUS       DAZFTN010000295         2253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFTN010000295 DAZFTN010000000
VERSION     DAZFTN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(140..784)
                     /locus_tag="ACHKV2_005321"
     CDS             complement(140..784)
                     /locus_tag="ACHKV2_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO6942999.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            882..1109
                     /locus_tag="ACHKV2_005322"
     CDS             882..1109
                     /locus_tag="ACHKV2_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6943000.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            1106..1531
                     /locus_tag="ACHKV2_005323"
     CDS             1106..1531
                     /locus_tag="ACHKV2_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6943001.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            1600..>2253
                     /locus_tag="ACHKV2_005324"
     CDS             1600..>2253
                     /locus_tag="ACHKV2_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO6943002.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
BASE COUNT          615 a          485 c          578 g          575 t
ORIGIN      
        1 gtattgcccg acgttctgtg gggcgaggta gggtggtatg taataaatct aaaagtattt
       61 agtttttcag tcaaggtaaa tctagattaa tttaaaaaaa aggtcggcat tgccgacctc
      121 aagtaaagga cgtggcgggt tataagttga actgtacacc ttttgctacg gcaacaattt
      181 tacactctgg tgtaagtaag gatgactgat agcgtggatt gagagggctt aaatacacaa
      241 gcttcccatc aataactaat ttctttatag tcatagatgg ttcattttta agcggatccg
      301 gaactattac agcgacgatg ctgccatttt tataactttc tcctggcctt aggatcacag
      361 tagcaccaac tgggatactc ggtgatcctg atgggttatg cattgtgtca tcaggcattg
      421 aaacggcaaa atcaccttcc tcgacatcaa agaatgtggt gattctatcg acatttccca
      481 ttgttttctc tccttttaag attaggaaag aaatcgcctc accccacgaa aggtagggga
      541 tcttggtgcc tgaggtactt tgtacaaaag ataattcagg agacgatact ccatatagga
      601 gataggactc agtagttcct aatgcctggg ctaacttact tagagctttg ctacctggtt
      661 cgtttagatc tttctcccag taccctatag taaccccagt cacgcctgaa agcttaccca
      721 gttctacttg ggtcagcccc ttatcttttc taagtctctt aagcctgaag ccaaggcttt
      781 ccatcatttt ctcctgcaaa ttgaatataa attattttag attgcattga cctaaaaaaa
      841 attatcttgt aatctaaaaa tacttagatt tgggaggtaa aatgcgagtt gatgaacttg
      901 tccagttttt tggttctgtt cagagggttg ccgattttta tgggataacc cgcgaagcta
      961 tttacatgtg gcgtaagcgt cctggtgaaa tagttccgaa ggggagggct gcggaagctg
     1021 ctgcatactc caaggggaaa ttatctttga acccagaact ttacaaaaag aaggatacca
     1081 ctcagggcga aggaaagggg gattcatgaa aatcaaacat gagcacatcc gcatggcgat
     1141 caatgcctgg gcctatccgg acggtgagaa agttcctgca gctgaaatag cccggactta
     1201 tttcgagctg gggatgacgt tcccggaact gtacgacgac agccatccgg aagccctggc
     1261 tcgtaatacc cagaaaattt tccgttggct ggataaagac acccctgatg ctgttgaaaa
     1321 aattcaggct ctgttaccgg cgatcgaaaa ggcaatgccg cctctgctgg tggcccgtat
     1381 gcgcagtcac agctctgaat attaccgtga gatcgtcgaa cggagggatc ggctggtgaa
     1441 agatgtggat gattttgtcg cagcggcgat cgcctggggc accctgacta acagtggtgg
     1501 tcagcctggt aatgctgttg tcgtgcattg accaacaata ttcatgccgg atttcttccg
     1561 gatgttcgag ggtaaagttc ggtatcagat gaggtgagta tggctaatgc ctggctcaga
     1621 ttgtggcatg acatgccaaa tgatcccaaa tggcgaacca ttgccagggt ctcaggacag
     1681 ccaatcgcaa cagtgatggc ggtgtatatc caccttctgg tgagtgcgtc acgaaatgtc
     1741 acgacatgtc acggcgtgtc actacgtggt cacattgatg tcacgacgga agatttagca
     1801 agtgcgcttg atgtgacgga agacgtgatt gattcaattt tgcatgcaat gcaggggcga
     1861 gttctggatg gtgatcttat ttccggatgg gaaaaacgcc aggtgatgaa ggaggataac
     1921 ggtaatgttt cgcaaaccgc aaaatccccg gcagagcgca agagagcgca gcgggagcgg
     1981 gaaagactgc ggaagcagaa cactgattgt cacgatgagt cacgacgcgt cacgcatatg
     2041 tcacgacaaa tcacgacaga tacagataca gataaagaat taaaccccac acataacgcg
     2101 cgcatgcgcg agagtgctac gaccggtgag ttgaatgacg cgtcgttgca gacagccgaa
     2161 cctgaatacc tggacggcct gagcgaaccg atcgggaaat ttccgatgac tggagtctgg
     2221 caaccgtcgc cggattttcg acagcgggca gca
//
LOCUS       DAZFTN010000296         2241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFTN010000296 DAZFTN010000000
VERSION     DAZFTN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1207)
                     /locus_tag="ACHKV2_005325"
     CDS             complement(<1..1207)
                     /locus_tag="ACHKV2_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6943003.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAAGRTSAFISGKA
                     PEIKPSPGGN"
     gene            1476..1592
                     /locus_tag="ACHKV2_005326"
                     /pseudo
     CDS             1476..1592
                     /locus_tag="ACHKV2_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1697..1773)
                     /locus_tag="ACHKV2_005327"
     tRNA            complement(1697..1773)
                     /locus_tag="ACHKV2_005327"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Arg,seq:tct)
     gene            complement(1787..1863)
                     /locus_tag="ACHKV2_005328"
     tRNA            complement(1787..1863)
                     /locus_tag="ACHKV2_005328"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1827..1829),aa:Arg,seq:tcg)
     gene            complement(1871..1946)
                     /locus_tag="ACHKV2_005329"
     tRNA            complement(1871..1946)
                     /locus_tag="ACHKV2_005329"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1911..1913),aa:Ile,seq:cat)
     gene            complement(1975..>2241)
                     /locus_tag="ACHKV2_005330"
     CDS             complement(1975..>2241)
                     /locus_tag="ACHKV2_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6943004.1"
                     /translation="TLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLC
                     SRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          529 a          620 c          568 g          524 t
ORIGIN      
        1 tgttgccgcc gggcgagggt ttgatttccg gtgccttacc actgatgaag gcggaggtgc
       61 gcccggctgc gttcagaata gcggttgcca gacgatccgg aataatgctc ctgcgcgccc
      121 atgaactgaa atgtgtcggg cggtttgatg atacctggtt tccattcgtt ctcgatgccg
      181 caccgtaata tcctgatgcc ggaatatccg gatcttctgc cggcgcgtta gtggcggtat
      241 tgacgccgtt accgtctgtc atgaagggca caaaataaac gccctcactc tccctgtttt
      301 tatacccgcc gtacacggtg tcgtactggg tagcgtatgt atttttccag taatacgtcg
      361 tatcaccaca aatccacggc acatctgcag cactgccacc atggcactgc gcgttaaaca
      421 cggagaggtc agcacgaaac tgtgtcagca tggctgtaaa cagcgcaggt tgctgtgcgt
      481 gggtggcggc gctcatgtca aactctccct gcatccagca caccgccagc aacacatttt
      541 tcgggttctt ctgtaatgca gctttagtgc gcgcaatcag gtcctgatat aacggtttac
      601 ccacacccca gcgtgccgaa tcctggctgg cccccgtgtc cgcactgaat gtcccctcag
      661 cgccctgggt gaatgcagaa ccaccacgac agcatggtac cagcaggatc cccgcgttat
      721 tcgggatata cggaagcagt tttttggcaa tatgtaagcc ctggccgaca cagccgtact
      781 gccctttgct caggtctgcc ttcggatgat tcagcgtact catatcctgc acatcatgca
      841 ggcagtggtc ggccggaatg atgtcgttat acgtacaact ctctccaccc ggagttaccg
      901 tgctgcggcg cgccagctgt ttaatgcgcg gatccggagc atcgtaagaa tccggtaacg
      961 gaagcccttc accgtaggcc atgccgttgg actgtccggc aagcacaacc acgtagaacc
     1021 agtccggctt agatgaaggg ccgacctgtg gatctccttc aatagccacc gcctgcatca
     1081 gtgtgtacgg cgtaatggca accggtccgc cgtatggctg ccagccctct ttcagtttgt
     1141 gtgtcagctt ttccgcaagg tctgacggcg acgccgccct gacaacatca tagtgtttaa
     1201 atgccatgaa tcctcccggc cgggataata ttgtgagtaa aatgaggagc gggctgaagt
     1261 ccggaagtta caggacaatg gcagaagaga gacaacagcc cgcaatacga aaaaggccgc
     1321 gctattgcgc agagtgatta ctgtcggata ttattcgcca gctgaaatat tacttcacgt
     1381 tttgttgttt attccttgcc gcccgcgtct ccctgcgcgg gctttttttg tccataagaa
     1441 agcccctccg gagaggggct ggagagtggc gctatgtgcc attgcatggt gccgggtgcc
     1501 tcccggtgaa ttcagtacca gcacctgaat ccgcgattat cccatatacc tactcgctga
     1561 ttgcccctcc gcacaggggg attcaccatg ccagtttctt ttaacaaact ccccgcaaac
     1621 cagacaacag tcaaccgcct gaattgtgag acatttaaaa aaaaggcccg caaaagcgag
     1681 ccagggaaaa taagtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa
     1741 ggcaattgct ctgtccggct gagctaacaa cgcaggatac agataatgga ccgccttcgg
     1801 ggacccgaac tccgcgcaac cagcttcgaa agctggcgct ctttcctgat gagctaatgg
     1861 cggtatgtga tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
     1921 cgctctcacc actgagctaa agggctgggt caaaaaataa taatcagatg aaaatcaata
     1981 atcaagccct tgcctggata catatctgtc tggcgggaag ccataatagc ggtgaaatac
     2041 agaaataaag taggaccagc ttgaataacc gcatttttct gctacagcct gtccatatcc
     2101 atgccgggaa cataacatat tgacagcaac acgcatccgc tcttccagca acaagcgact
     2161 gaacatgtgc ccttcatttt tcagttttat ctttaacaaa ctctcactca tatgcaggcg
     2221 taacgcaatc gcaccaagcg t
//
LOCUS       DAZFTN010000297         2102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFTN010000297 DAZFTN010000000
VERSION     DAZFTN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..233)
                     /locus_tag="ACHKV2_005331"
     CDS             complement(<1..233)
                     /locus_tag="ACHKV2_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO6943005.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLS"
     gene            complement(868..1329)
                     /gene="yibG"
                     /locus_tag="ACHKV2_005332"
     CDS             complement(868..1329)
                     /gene="yibG"
                     /locus_tag="ACHKV2_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO6943006.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1341..>2102)
                     /locus_tag="ACHKV2_005333"
     CDS             complement(1341..>2102)
                     /locus_tag="ACHKV2_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6943007.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          593 a          460 c          367 g          682 t
ORIGIN      
        1 gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag tccgagttca
       61 gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgtcc ctctttagta
      121 ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc tctctgaaat
      181 tgtcttgcag taactgcact cgcagaaata gagaaaaaaa tacaattgtt aataaccttg
      241 ccatattttt tagtccgtaa ctgttaatgt gctatctggc tctgaaaata ttaactcatt
      301 taatttttgc atgtcattag ataaaccagt tatacaaccc tcggaacaga atcgaggtcc
      361 taaagacgga ccaaaagcgt ttaaacaact atgtattgaa aatccattgc gattataagt
      421 tcctgtatta actgacggta ctaatcgtat gccgtcaggt tttctattgc ttgctcccgg
      481 gtcattaaca ttccaactcc ataggccttg agatattggc catctattta taaatgtgta
      541 caacggggat tatttttaca ttgcatatta ttaccagaag cgactggaat actaagcaca
      601 tcataaaaat tatcatcaca tggtatatct gaaacacacg aaagcatacc taaagttatg
      661 ctgtataaac aggtggctaa tcccaagggg atcgattcca gaaatcgggt tcaacggata
      721 ctgataaaaa ttccacctac cttccagccc aatcggagcc tgagtgttat accgcccctg
      781 ctgcggttaa tgttaccgat aatagttata gtacagccct gattcatcat catactactg
      841 ctgcggcagt ctgatgaact attgctttta cggattctcc ttattcttcc ttctatccat
      901 tcgctttagc tttttgagct cttgaagttt gctctttgca tcaatattac cgttatttac
      961 agcgaattcc atccaataaa tagctctatt caaattataa tattctgata atgttggatt
     1021 tgtatctgat aaaaagaccc cataattaaa ctgcgctgta acattcccct gagatgctga
     1081 tttttctaag tatcgcaatt gtttgtaaat gttattttta gtaaagcagt aatattgata
     1141 tagtcgaaat gatgcctctg aatcaccatg ttgtgattta tacttcagtt tcatcaactt
     1201 ttcttcactt aaatcatagg tcattgctgt agaccccatc atactttcgt tttgttttat
     1261 tttcacatca gcaccatgca attgacaaat aaaagagaaa taaaaaaata gtaacaagca
     1321 tgctttcata catctacaac ctatcttata tcactgggtt tttctggcgt accttgcata
     1381 ttagaaatgg ggtgcccaga tggcccatat gttatccaat taccaaggtc ttttgttgtt
     1441 atagccatta gatccttatt tgaattaagg ttcgttggtg ctgttagcac tgtatccatt
     1501 tttaacttac cattagttcc tcgcccccac cacgtccata gcgtgtcagg tcctattaca
     1561 tttgtatgta actctttagc taattggcta gcaaatgaat tttgtccttt acctgtatta
     1621 caagaaaata accaaactgt catcccatct ttataattcc catcaaattt aattagatat
     1681 gctagatctt tagctgtcat gatactgcgc gttgcggatt caatagatgt gggggtacca
     1741 tgccccccga ttgtgaaaac gcctgggata ttaatctcat cagctacaga atggataaga
     1801 tcactttcgg ggtataaatt tatatcaacc aaaccttgcg gatctacatt tatgaccgga
     1861 ttcaacggat actgataaaa attccatccc cccttcagtc caatcggatc ctgagtgata
     1921 tatcgcccgt gcagcgggtc ataatagcgg tggcggttgt aatacaggcc ggactcctca
     1981 tcatactgct gccccggcag gcggataagc tgctgcagct gatgcgggtt ctcttcattc
     2041 agcaggttgc cccattcatc gtattctgcg caccattctg ttgccccttc cgcactgata
     2101 ag
//
LOCUS       DAZFTN010000298         2086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFTN010000298 DAZFTN010000000
VERSION     DAZFTN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..2086
                     /locus_tag="ACHKV2_005334"
                     /pseudo
     CDS             2..2086
                     /locus_tag="ACHKV2_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          732 a          408 c          386 g          560 t
ORIGIN      
        1 tatgttttct ataaaaccag gaccaagaaa attacctatc gacaacccct cattgttatc
       61 atggaacatt actgacgggg atctaaattc caaattaaat acattagaat atctaaactg
      121 tgtaacaaat attattaatg cttgtggagt ttatcctcag gatttaaaag acagagaaat
      181 tatatcaact tttcacgcag aaaaagtcat taatgatctg ttaaaaaacg attataaaat
      241 ttccctttct ccagatacaa cttaccgaga gctgaataaa gctgcacagc gtagcattac
      301 agcgccagac aggataggag aagggaaaat atgggtttat caacgagata caatggttga
      361 aagaggtgat aacagcggtg ttcatcagta tggtccagct gaacatttca ctcacattat
      421 atctgacaaa ccgtccccaa aagataagta tgttgcatat gctattaaca ttcctgacta
      481 tgagctagca gccgatgtat ataatattaa cgtgacgtca ccttccggac agcaagaaac
      541 atttaaaata ttaatcaatc cagaacatct acggcaaaca cttgagcgta aatcccttac
      601 tgctgttcag aaatcacaat gtgaaatcat cacccccaaa aaacctggcg aagcgattct
      661 tcatgctttt aatgccacct accagcaaat cagggaaaat atgtctgagt ttgcacgttg
      721 ccattatggg tatatacaaa tccctccagt gacaactttc cgcgccgacg gaccagaaac
      781 tcccgaagaa gaaaaaggtt actggtttca cgcttatcaa cccgaagatc tttgtaccat
      841 ccataatcca atgggagatt tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt
      901 tggtatcgat atcattcctg attatacctt taactttatg ggaatcgggg gtagtggtaa
      961 aaatgacctg gattatccct ctgctgatat acgagcgaag atcagtaaag atatagaagg
     1021 tggtatccct ggctattggc aaggtcaggt tttgattcca tttactatag atccagtaac
     1081 aaaagaacgt aaacaaatcc atccagaaga tatacatctc acagcaaaag acttcgaagc
     1141 aagtaaagat aatatttcta aggatgaatg ggaaaacctc catgcattaa aagaaaagcg
     1201 tttaaatgga atgcctaaaa ccccccccaa aagtgactag gttattatgt tgcaaaatca
     1261 atacgttcgt gaaatgcgaa aatatggcgt acgaggttta cgttacgatg cagcaaaaca
     1321 ctcaaaacat gaacaaatag aaagatcaat aaccccaccg cttaaaaatt ataatgagcg
     1381 attacacaat actaacttat ttaacccaaa atatcatgaa aaagccgtta tgaattacat
     1441 ggaatatctg gtaacttgcc agttggatga agaacaaatg tcatcgctgc tttatgaaag
     1501 agatgattta agcgccattg atttttcatt gctcatgaag acgataaaag ctttttcatt
     1561 tggtggcgat ctccaaaccc ttgcatcaaa accgagttcc acaatctcaa gcatcccatc
     1621 aaaaagacgg atattgatta acattaacca cgattttcct aacaatggca atcttttcaa
     1681 tgactttcta tttaaccatc aacaagatga acaattagca atggcatata tggccgctct
     1741 cccgttcagc aggcctttag tttactggga tggccaagta ttaaaatcaa cgactgaaat
     1801 taaaaattat gatgggtcga cgcgtgtcgg cggtgaggcg tggcttaata aaggttgctc
     1861 tacctatcag cagctctaca atgaattcca cgcattatat atagataaag caggaatatg
     1921 gagcgcattt gagggtgtat ttgcaactaa aaacgttctg gcctttagtc gtggggattc
     1981 ggtgaacatt aatcactctc ctcatgatgg actagttata ataaataaag gaaacgaaga
     2041 agttgaaggt gcctggccta acaaattgca acctggaaaa tataaa
//
LOCUS       DAZFTN010000299         2080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFTN010000299 DAZFTN010000000
VERSION     DAZFTN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            110..625
                     /locus_tag="ACHKV2_005335"
     CDS             110..625
                     /locus_tag="ACHKV2_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO6943008.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELM
                     KAVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRR
                     ATGWQRVETRA"
     gene            730..1272
                     /locus_tag="ACHKV2_005336"
     CDS             730..1272
                     /locus_tag="ACHKV2_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943009.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVL
                     QAIEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDL
                     QIRGLSIDQLVKITGYLKQL"
     gene            1269..1517
                     /locus_tag="ACHKV2_005337"
     CDS             1269..1517
                     /locus_tag="ACHKV2_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943010.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYD
                     AVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNET"
     gene            1507..1734
                     /locus_tag="ACHKV2_005338"
     CDS             1507..1734
                     /locus_tag="ACHKV2_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6943011.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQ
                     NSQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
     gene            1827..>2080
                     /locus_tag="ACHKV2_005339"
     CDS             1827..>2080
                     /locus_tag="ACHKV2_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943012.1"
                     /translation="MHQGGVNSLALALTAAFCGANQHNHGLLVSWALQGEGMACAPWY
                     VRQRLRRASGRCGSCAAQVFSFAPSIRLRGLKNVITITYRV"
BASE COUNT          675 a          386 c          512 g          507 t
ORIGIN      
        1 aaaaaaacgg gagaaaactt gcaaatacga cttaaaagtc gtattgttat ttcatcgaaa
       61 ggggaatggc ccctctcaca atagcccaac agggcaggag attaaaaaaa tgaatatcca
      121 ggaagcatta aacgtttttg gattatccgg cgaattaact gaaaaagata tcaaagcagc
      181 atacagaaaa gccgctttaa aatatcatcc agatcgtaac ccgttagggg ctgaactgat
      241 gaaagcggta aatgcagctt ttgatgtatt gatggcaaat attgataaaa taaatcagtt
      301 ccagagcact gatgaacatg cacgatataa ttacggtgat gacctggaaa aagtattaaa
      361 cgttctttct ggtttatctg gtctggtatt tgaagtgata ggtaactggg tctggattag
      421 tggagaaacc attacacata aggaaacttt aaaagaaatc gggtgtaaat gggcggcaaa
      481 gaaaaaacaa tggttttatc gtccagacga acataaaagt tactggaatc gtgaagaaca
      541 cacgatagaa gaaatccgcg caaaatacgg tacaaccgga cagcgcaggg cgacagggtg
      601 gcaacgcgtg gaaaccagag cgtaaccaga acgggggcga gaagcccccg atattatagc
      661 cagcccgtct tttggggcat cgaacccaaa aagacgggaa cagcagctac gtagcaaaaa
      721 gggaaacaga tgaactcttt ttttgaacaa tatcaccctg tctttgaagt tgtctgccgt
      781 atcctgggga acggctggcg cgtgaacaaa cttgatgatt gttcgtctcg tataaaactg
      841 acgtcaccgc agtttaaaaa ttactctgtg catattcgga tggaaaaaga ccggttttct
      901 gttgtgggga gtgtggatag tcgctcctgg cgtagtccgc atcatgtttg cacgttatcc
      961 agaaaacgga atcccgttga tattgcagcc gatatcgagc gaaaaattct ggtaaatgct
     1021 tcgcaggagg tgttacaggc gattgagtat gagaaacacc aagtggaaaa aaaggacgaa
     1081 attctgatcc tgaaaggtat gttatcgcaa cttgtccagc ttgaaagctg gtatggggca
     1141 ttgacaggct ttaaagctga aaacggattg aacggtaaag tcaccgaaca gggcgacagt
     1201 tatgatttac agattcgggg cttgagtata gatcaacttg ttaaaattac agggtatttg
     1261 aaacagttat gagaaaaaat aaaggcgatg tgacttattt tcttgaaaaa gaaggtgaca
     1321 attacaggct tacaaaaagg ataaaagcca gaacaaatgt taaaatcggg aacaaaacta
     1381 cgaaaattac attgtatgat gccgtactga atgagaatga attacaacat attgatttta
     1441 cgtgtgccgg attaagggaa gacgatgaga cgccagttaa aaatttaatt aaggagttta
     1501 tgttaaatga aacctgataa tacacctgaa aatgtaaaaa aattacgttt aaaagcagga
     1561 ttaacacaaa aggaatgcag tcatatttat ggtgttgggc taagaacctg gcagaaaaaa
     1621 gaggaagtta atactcaaaa cagtcaaagt ctgtcactgg ttgagtttga gtttcttttg
     1681 ttacttgcag gagaacatcc tgaatacgtg ctctgcaaaa gggagtcaaa ataaaatgac
     1741 ctatgatggt tttatattag tacctggagt tgcgcccgta gctcgctgac gctcacacgc
     1801 gcagtacagc gccccgccat gctgcaatgc accagggcgg ggtaaactcg cttgcgctcg
     1861 cgctaactgc ggcattctgc ggcgcaaatc agcataatca tggcctgctg gtcagttggg
     1921 ctttgcaggg tgaagggatg gcctgtgcac cctggtacgt tcgtcagcgg ctgcgccgcg
     1981 cttcggggcg atgtggctcc tgtgctgccc aggtattttc gttcgcccct tccatccgct
     2041 tacgcggatt aaaaaacgtg ataacaatca cttatcgagt
//
LOCUS       DAZFTN010000300         2060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFTN010000300 DAZFTN010000000
VERSION     DAZFTN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            171..1532
                     /gene="espK"
                     /locus_tag="ACHKV2_005340"
     CDS             171..1532
                     /gene="espK"
                     /locus_tag="ACHKV2_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO6943013.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            1593..1868
                     /locus_tag="ACHKV2_005341"
     CDS             1593..1868
                     /locus_tag="ACHKV2_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO6943014.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(1948..>2060)
                     /locus_tag="ACHKV2_005342"
     CDS             complement(1948..>2060)
                     /locus_tag="ACHKV2_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO6943015.1"
                     /translation="YFNFTTLHDYNHFCDFIEFKHKNIIMNTSQYTGSSW"
BASE COUNT          756 a          436 c          360 g          508 t
ORIGIN      
        1 actggagaaa gtattcgcaa aagttaaaat caacagtctg ttggttttga gatataactc
       61 tcttttctaa tattgacacc ttggctttgc tggttgttaa aattagcttg ttgattagta
      121 gtatggatag aacttactct gttatttatt tctaaaaatg gaatttatca atgcttccta
      181 catcgcaatt acgaccgacc gggacattct gctcctattc cgctgaaaca tcagcagaca
      241 tcaaaagcga aatcacacca attcagatag aagaagcgcg ggccagtggt cgtttatata
      301 tcaaagattg tgatattgag tatctgccac agttaccaaa cgaaataaca tcagttacaa
      361 tcgaaaactg caacaacctg acaaccctta caggattgcc ggttaataca caaaacctct
      421 ccgtcattaa ctgtgaaaaa ttacaaatca cagacatgcc atcaaccgta aaaaatctac
      481 atattgaatt aactgattca ccatttatac atttcatatc tgaaggcatc gagtgcctga
      541 cggtttgcca ctgctatata tctggagtgc cagagagtgt ccgctacctt gaaataaaag
      601 gtagcgccac agacagcata aaaaatgttc caaacgggtt atcatctctc agcatcaata
      661 gctataaccc ggagaatcag gccagaattg acaacctgat atcaccgtca ctgaagacgc
      721 tatcgctgac tggatgtagc aatattatac tgccggagaa acttccggag agtgtgacat
      781 cggtaaccat tcatgcggag cagaaaacca cgtggaacat cggtgttgaa gggatgcctg
      841 atgggctgga tcttgattta caaaatgtac tactctctcc agatgtagtt aaagcaaaaa
      901 acatcacctt tcagggcaac gctctggatg tggccttaca ctttcgcgag ggagacattg
      961 tctatggact atcttcaccc agagaaaaac ttgtcaacag cattaaacta gttaacgact
     1021 tttccaaaaa agatattata actcagaata cgttaacaaa cgcagtatgg gaccccagaa
     1081 cacctcgcaa atataagcaa gatccactta tcaaaagagc attaaatgaa cacgaaagag
     1141 gaataaaatt taaacaacac ttaaagaatc acaataatta taatgttacc atggccgacc
     1201 tttccgtata caatcgcgac aaattatggg caaaaacaag caaggccggc ctagagtttc
     1261 agacattaac acgcaataaa acggttattt tttgtgcgga tgagcttgtc aactcactca
     1321 aactcatagc taacaagtca gagggctatg gccagagtat taccgccagc gaattacgat
     1381 ggatttaccg taataaagac aacaaccaaa taatgaaaaa cataaaattt tatctacatg
     1441 gcaaagagat accagcagaa agaatattag atacaccaga atggaaagac tatcgtccaa
     1501 aatactctgg ttccacatat aaatattctt aatgatacca acttataacg gaatagcata
     1561 aaaacacttt tcatggagca aaggagaaaa caatgccatt ttcaatcaaa aacagatttt
     1621 caagttcaca agtacattac ccggaaatat ccggtcccat aaaagacaag ccagcgtcaa
     1681 agaactgcat acttacatca acaacatgta atgtagatag ctatacagtg taccaaaaaa
     1741 aagcctgtag ttttgacatg cgcccacccg gcgcaggaga aagaacccca aaactaaaac
     1801 tctcagttac tgagatgaca tggctatcta aaactataga aacagagata cacaacacaa
     1861 aagaatagca accacttacc agagaacaca aaaagccaca gacccgaaac acctgactgc
     1921 aagcaaccac ctccacagga ggtggtttta ccatgaactt cctgtgtact gacttgtgtt
     1981 cataataata tttttgtgtt taaactcaat aaagtcacaa aaatgattgt aatcatgcaa
     2041 tgtagtaaaa ttaaaatatt
//
LOCUS       DAZFTN010000301         1975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFTN010000301 DAZFTN010000000
VERSION     DAZFTN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1183
                     /gene="escC"
                     /locus_tag="ACHKV2_005343"
     CDS             <1..1183
                     /gene="escC"
                     /locus_tag="ACHKV2_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO6943016.1"
                     /translation="SLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLS
                     ESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLP
                     STGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQIEIS
                     VSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRVNAL
                     QQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRIL
                     DDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGE
                     SLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASS
                     E"
     gene            1197..1652
                     /gene="cesD"
                     /locus_tag="ACHKV2_005344"
     CDS             1197..1652
                     /gene="cesD"
                     /locus_tag="ACHKV2_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO6943017.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
BASE COUNT          658 a          373 c          379 g          565 t
ORIGIN      
        1 ctcactgcta aaatatctga gtgatacaat ctcagtaaac aaaaactctt gcaatgtgag
       61 aaaaattacg acatttaact cgattgaagt tcggggtgtt cctgaatgta taaaatatat
      121 tacatcttta tcagaaagtc ttgataaaga ggcacagtct aaagccaaaa ataaagatgt
      181 tgttaaggtc tttaaactga actatgcgtc agcgacagat ataacataca aataccgtga
      241 ccaaaatgtt gtcgtcccag gtgttgtctc cattctgaag acaatggcaa gtaatggttc
      301 acttcccagt accggtaaag gtgccgtcga gcgaagcggt aacctgtttg ataacagtgt
      361 aacgatttcc gctgatccac gtcttaatgc agttgttgta aaagaccgcg aaataacgat
      421 ggatatctat cagcaattaa tttctgagct tgatattgaa caaaggcaaa ttgaaatttc
      481 tgtctcaatt attgatgttg atgcaaacga tcttcagcag ctcggcgtaa actggtccgg
      541 tacgttaaat gcaggacaag gtacgattgc ctttaatagc tctaccgcac aggctaatat
      601 cagctcttct gtgataagta atgccagtaa ttttatgatt agggtgaatg cgttacaaca
      661 gaactcgaaa gctaaaatac tctctcagcc ttcaattata accttaaata atatgcaggc
      721 gattcttgat aaaaacgtaa cgttttatac aaaagtctcc ggagaaaagg tggcgtctct
      781 ggaaagtatt acaagcggaa cattgctgag agtgacacca cgtatacttg atgacagctc
      841 gaatagcctt accggaaaac gtcgggaacg agttcgtttg ttacttgata ttcaggatgg
      901 caatcaatcg actaaccaaa gcaatgctca ggatgccagc agcaccttac ctgaagtaca
      961 aaattcagaa atgacaacag aagcgacatt aagtgcagga gaaagtttat tacttggcgg
     1021 atttatccaa gataaagaaa gttcaagtaa agatggtata ccgctgttaa gcgacattcc
     1081 tgttattggt agtttattct ccagtacggt aaaacaaaaa catagtgttg ttcgactctt
     1141 tttaattaaa gcaaccccga taaaatctgc atctagcgaa taataaatga ggctttatga
     1201 gcaggaaatt tagctctcta gaggatattt atgatttcta ccaggatggt ggcacattag
     1261 cgtcattaac aaatctgaca caacaagatc tcaatgacct tcattcttat gcctatacag
     1321 catatcaatc tggtgatgtt ataaccgcaa gaaatctatt ccatttgctc acatatctgg
     1381 aacactggaa ttatgactac accttatctc tgggcttatg tcatcagcgt ttatcaaatc
     1441 atgaagatgc acaactgtgt ttcgcacgct gtgcaacttt agttatgcaa gatcccaggg
     1501 catcttatta ttctggaatt agctacttac tcgtcggaaa taagaaaatg gccaagaaag
     1561 cctttaaggc ttgtttaatg tggtgtaatg aaaaagaaaa atacactaca tataaagaaa
     1621 atattaaaaa attgttaggt aatacagagt aaaaactacc atcagctata ccaatcccca
     1681 ggctgactgg ggattttcta tcggaaataa taaagctgct atagtgaagt gctcccaaag
     1741 ttgagcaccg accactggta gatttatcta caggtgtggg gttattccat taaccaggag
     1801 tttatgccct gaactatctt ctgctgtatt taatatcatt tccgtttatg aaacaatcag
     1861 tatatcttaa cttagcatta gaaatctgat ctttccaatc atctatagct cagagtatat
     1921 ttgtcaggaa atgaccttca aactttagcc gagaaaaagg cttaccctgg aaaac
//
LOCUS       DAZFTN010000302         1940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFTN010000302 DAZFTN010000000
VERSION     DAZFTN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..171
                     /locus_tag="ACHKV2_005345"
     CDS             <1..171
                     /locus_tag="ACHKV2_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943018.1"
                     /translation="DLLYNESRPGGLVLPSSFWRAVAGVKGLEDFEVRSPLKSVMAGD
                     TELLTVGEITWL"
     gene            162..722
                     /locus_tag="ACHKV2_005346"
     CDS             162..722
                     /locus_tag="ACHKV2_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO6943019.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            722..1633
                     /locus_tag="ACHKV2_005347"
     CDS             722..1633
                     /locus_tag="ACHKV2_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943020.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(1668..>1940)
                     /locus_tag="ACHKV2_005348"
     CDS             complement(1668..>1940)
                     /locus_tag="ACHKV2_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6943021.1"
                     /translation="GNWQFSDGLISKVPVNWKTVAEKRRSSLLQEANETVDDWKTELK
                     LDMISDENKLQLTRWMAYIRQLKEMHFNDIASEGHYQAIPWPEKPE"
BASE COUNT          471 a          464 c          517 g          488 t
ORIGIN      
        1 gacctgcttt ataacgaatc gcggccgggt ggacttgtat tgccttcatc attctggcgg
       61 gctgttgcag gggtgaaagg actggaggat tttgaagttc gcagcccgct gaagtccgtg
      121 atggccggag atacagagtt gctgaccgtg ggggaaatca catggctgta accctgaccc
      181 cgcatcagcg cgccctgttg cagttgctgc ctgacgggct ggcatgggat aagcggccgt
      241 catccgttct tgcggctttg tgcctgggcc tcagtcattc cacggagcgt gtttcctgga
      301 ccggtaacca gatgctggca gaacgttttc ctgattcatc ccgtctgctg ctggaagact
      361 gggagcgtta tctggggtta ccggaatgtg atatgaccgg cgcaaccatt caggagcgtc
      421 agcgttatgc cgggaataaa taccggatga aaccctctct taaccgtgaa ttttatatcc
      481 ggtttgcggc agagtttggt tatgaaatag atattcagcc atcaccggat tcacagtggg
      541 tcagtattgt cacgattaac agtgaaaccg gctaccggaa tatgaatgtg ctggatgata
      601 ttctcacgcc gctgcgtatt tatgaaggcg gtgcgctgga atgtattctg aaccgttata
      661 agcctgcatg gcagacgttt atttacgtat atgcaaacag tcacgaagag gagaatattt
      721 aatgttccat gttgataata attccggcgt ggcgaatatg cctgcgctgg caccggcgca
      781 gagtaatacc accacctggt ttaccgaagg tgacggacaa aaaggtatca gctggattgg
      841 tcaggactgg ctgaatattc tccaggccga actgctgaat attctggctg aagccagtat
      901 tcagccggat aaggcgcagt taaaccagct tacgctgtcc attaaagcca ttatcgctgc
      961 gaatgccttt tcccggaaaa acaacctgaa agaaattgct gatgccggtg cggaagccca
     1021 gcgtcttgcc cgtggttatc ttggtcttgg ggcgcttgcc acaaaaaaca gtcttggtcc
     1081 cggtgacgtt aatgctctgg cgaaggatca gaaccttgcc gacctggaga atgcgggaac
     1141 cgcccgtaat aatctggatg tttacagcaa aagcgaaggt gataaccgtt acctgcgcag
     1201 ggagcagaac ggcgcagaca ttccggataa aggcgctttt atcgataacg tcggtttacg
     1261 ggaaacggta aataaggctg ctgatgccct gccatcgggt ggaaccgccg tggcagcaaa
     1321 cagactcgcc accccaagaa atattaatgg tgttcctttt gacggaacgc tggatatcaa
     1381 catcacgtca ggaatgacgc agtcaaccgc agatggccgg tatgtacaga atgttcagct
     1441 tggagcacag agctatcatt cacccggagg caatgaaatg tcatggaatt acagcgcacc
     1501 ttccggttgt atgctttccg gaattaacgt gcaggaaacc ggctcccggt ctgcggacaa
     1561 tatcggtggg gtctattatc gcccggttca gatttatatt aataatgcct ggagaacggt
     1621 aagttcagtg taaaccacag aaaagggtgc tgaatgcacc ctctgaatta ttccggtttt
     1681 tccggccagg gaatcgcctg gtaatgcccc tcactggcaa tatcattaaa gtgcatttct
     1741 ttaagctgtc ggatatacgc catccagcgc gtcagctgta atttattttc atcgctaatc
     1801 atgtccagtt ttaattccgt tttccagtca tccacagttt cgttggcttc ctggagcagc
     1861 gatgagcggc gcttttcagc aaccgtcttc cagtttaccg gcacctttga tatcaggcca
     1921 tcagaaaact gccagttacc
//
LOCUS       DAZFTN010000303         1929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFTN010000303 DAZFTN010000000
VERSION     DAZFTN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..969
                     /locus_tag="ACHKV2_005349"
     CDS             <1..969
                     /locus_tag="ACHKV2_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO6943022.1"
                     /translation="NHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASP
                     ENFRFSEERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDP
                     GLTGMNPVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAA
                     LSALTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEY
                     VALSAQHGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAAL
                     TGLQTDTVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            969..1916
                     /locus_tag="ACHKV2_005350"
     CDS             969..1916
                     /locus_tag="ACHKV2_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO6943023.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
BASE COUNT          433 a          546 c          550 g          400 t
ORIGIN      
        1 aaccatgcag ccgttgagcg catggtctcc cgtgttgtgg cgctcaatca gccggtgaaa
       61 attgactaca accaccagac cctgattgag ggacatccgg caccggctgc cggttttgtg
      121 atggcctcac cggagaattt tcgcttcagt gaagagcgag gtttcgaggt gcgcccgaag
      181 tggaaccctc cggcccttga acatctgcgt aataacgaat tcccctggtt ttcaccggtg
      241 attggctatg acgagagcac cggcgaacct gtcgaacttc ggatgctggc tattaccggt
      301 gaccccggtc tgaccggcat gaatcctgtc gccgcactgt cggcggatga cctttataac
      361 gccttaaacc ctcctttaaa ggatacctcc atgaatgagc aattacgcca gttgctgacg
      421 gcgctcggtc tgaccgtggc tgacggtgac gaatttacgc cggagctggg cacggcagcg
      481 ctgtctgccc tcaccgggat caaaacccgt gcggatgcac acgacaacct gaaaacacag
      541 gtcgccagtc tgtcggcaga gctggaaact gcaaaaggca caccggctgg cggcagtatt
      601 gacctgacga aatacgtgcc cgttgaaacg tataacgccc tgcgtaccga atacgttgcg
      661 ctgtcggcgc agcacggcag caccacgctg gagcagttgc tggacaaggc cgagtccgaa
      721 ggacgcatct tcaaaagtga acgcggctat ctggaacaac tgggcgggca gattggcgtg
      781 gcggcacttt ctgcacagct tgacgcccgc cagccggttg cggccctgac cggccttcag
      841 accgacaccg tgaccgtgcc ggataaaaag actgccaccg ctgtgctgtc ggctgaagat
      901 atcgcggccg ccaaactgct gggtaaaacc gaagccgagt tcctgaaaat gaaagaggaa
      961 atgcaataat gcctaccccg attacaccgg cgatgatcac cgccctgatg acgggctacc
     1021 gttctgattt tcaggccggg atgtccatgg ccccgtcgca gtacaaaaaa attgcgatga
     1081 ccgtgccctc cacatcgaaa tccaacacct acggctggct ggggcagttc ccgcagttcc
     1141 gtgagtggat cggctcccgc gtcatcgaga agatgaaggc ctatggctat gccatcgtga
     1201 acaaaacctt tgaaggcacc gtcgccatta accgcgatga cttcgaagac gataacctcg
     1261 gtatttattc cccgttgttc caggagatgg ggcgcgctgc tgcggcacaa ccggatgaac
     1321 tggtctttgc tgctcttcgt gacgggatta atgcggcctg ctatgacggc cagaacttct
     1381 ttgataccga acatcctgtt taccccaaag tggatggctc cggggatgcg cagatggtca
     1441 gcaatatgtt tgtggcaaaa accggctctg tcggtgcaca ggcggattac agcggtcctg
     1501 cctggtatct gctcgactgt tcacgcgcaa tcaaaccgct gatttatcag gatcgccgta
     1561 aggctgaact ggttgcccag accaaagtcg atgaaggtcg cgcattcacc gataacgaat
     1621 ttgtgttcgg tgcttccgcc cgtcgcaatg tgggctacgg cttctggcag atggcctaca
     1681 tgatgcagtc accgctgacg ctggatgcac tgtggcacgg ctggtcagcc atgcgcgaat
     1741 ttaccgctga cggtggccgc aaactcggca ttaaacccac ccatattgtt gtccccacct
     1801 cgctggaaaa acaggcggtg cagttgctgg agcgtgaact gttcgcagac ggaaacgcaa
     1861 ccgtctctaa cgagatgaag ggcaagctgg agctggttgt cgcggattat ctgtaaacgg
     1921 tgagccggg
//
LOCUS       DAZFTN010000304         1929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFTN010000304 DAZFTN010000000
VERSION     DAZFTN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..969
                     /locus_tag="ACHKV2_005351"
     CDS             <1..969
                     /locus_tag="ACHKV2_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO6943024.1"
                     /translation="NRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASP
                     ENFRFSEERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDP
                     GLTGMNPVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAA
                     LSALTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEY
                     VALSAQHGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAAL
                     TSLQTDTVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            969..1916
                     /locus_tag="ACHKV2_005352"
     CDS             969..1916
                     /locus_tag="ACHKV2_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO6943025.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
BASE COUNT          430 a          543 c          557 g          399 t
ORIGIN      
        1 aaccgtgcga ccgttgagcg catggtatcc cgggttgtgg cgctcaatca gccggtgaaa
       61 attgactaca accaccagac cctgattaag gggcatcagg caccggctgc cggttttgtg
      121 atggcctcac cggagaattt tcgcttcagt gaagagcgtg gcttcgaggt gcgcccgaag
      181 tggaaccctc cggcccttga acatctgcgt aataacgaat tcccctggtt ttcaccggtg
      241 attggctatg acgagaacac cggcgaacct gtcgaacttc ggatgctggc tattaccggt
      301 gaccccggtc tgaccggcat gaatcctgtc gccgcgctgt cggcggatga cctttataac
      361 gccttaaacc ctcctttaaa ggatacctcc atgaatgagc aattacgcca gttgctgacg
      421 gcgctcggtc tgaccgtggc tgacggtgac gaatttacgc cggagctggg cacggcggcg
      481 ctgtctgccc tcaccgggat caaaacccgt gcggatgcac acgacaacct gaaaacacag
      541 gtcgccagtc tgtcggcaga gctggaaacc gcaaaaggca caccggctgg cggcattatt
      601 gatctgacga aatacgtgcc cgttgaaacg tataacgccc tgcgtaccga atacgttgcg
      661 ctgtcggcgc agcacggcag caccacgctg gagcaggtgc tggacaaggc tgaatccgaa
      721 gggcgcatct tcaaaagcga acgcggctat ctggaacaac tgggcgggca gattggcgtt
      781 gcggcacttt ctgcacagct tgacgcccgc cagccggttg cggccctgac ctctcttcag
      841 accgacaccg tgaccgtgcc ggataaaaag actgccaccg ctgcgctgtc ggctgaagat
      901 atcgcggccg ccaaactgct gggtaaaacc gaagccgagt tcctgaaaat gaaagaggaa
      961 atgcaataat gcctaccccg attacaccgg cgatgatcac agccctgatg acgggctacc
     1021 gttctgattt tcaggccggg atgtccatgg ccccgtcgca gtataaaaaa attgcgatga
     1081 ccgtgccctc cacatcgaaa tccaacacct acggctggct ggggcagttc ccgcagttcc
     1141 gtgagtggat cggctcccgc gtcatcgaga agatgaaagc ctatggctat gccatcgtga
     1201 acaaaacttt tgaaggcact gtcgccatta gccgcgatga cttcgaagac gacaacctcg
     1261 gtatttattc cccgttgttc caggagatgg ggcgcgctgc ggcggtacaa ccggatgagc
     1321 tggtctttgc tgccctgcgt gacgggatca gtgcggcctg ctatgacggt cagaacttct
     1381 ttgataccga acatccggtt taccccaaag tggatggctc cggggatgcg cagatggtca
     1441 gcaatatgtt tgtggcaaaa accggctctg tcggcgcaca ggctgattac agcggtccgg
     1501 cctggtatct gcttgactgc tcccgcgcgg taaaaccgct gatttatcag gatcgccgta
     1561 aggctgaact ggttgcccag accaaagtcg atgaaggtcg cgcattcacc gataacgaat
     1621 ttgtgtttgg tgcatccgcc cgtcgcaatg tgggctacgg cttctggcag atggcctaca
     1681 tgatgcagtc accgctgacg ctggatgcac tgtggcacgg ctggtcagcc atgcgcgaat
     1741 ttaccgctga cggtggccgc aaactcggca ttaaacccac ccatattgtt gtccccacct
     1801 cgctggaaaa acaggcggtg cagctgctgg agcgtgaact gttcgcagac ggaaacgcaa
     1861 ccgtctctaa cgagatgaag ggtaagctgg agctggttgt cgcggattat ctgtaaacgg
     1921 tgagccggg
//
LOCUS       DAZFTN010000305         1906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFTN010000305 DAZFTN010000000
VERSION     DAZFTN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(218..385)
                     /locus_tag="ACHKV2_005353"
     CDS             complement(218..385)
                     /locus_tag="ACHKV2_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6943026.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(382..813)
                     /locus_tag="ACHKV2_005354"
     CDS             complement(382..813)
                     /locus_tag="ACHKV2_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO6943027.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(983..1059)
                     /locus_tag="ACHKV2_005355"
     tRNA            complement(983..1059)
                     /locus_tag="ACHKV2_005355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1023..1025),aa:Arg,seq:tct)
     gene            complement(1160..1235)
                     /locus_tag="ACHKV2_005356"
     tRNA            complement(1160..1235)
                     /locus_tag="ACHKV2_005356"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Ile,seq:cat)
     gene            complement(1264..>1906)
                     /locus_tag="ACHKV2_005357"
     CDS             complement(1264..>1906)
                     /locus_tag="ACHKV2_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6943028.1"
                     /translation="LKPGSVVLATKCIRELFLMHYGKVKIVDISESVVSQYLESQHKL
                     TRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFL
                     SGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEER
                     MRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          507 a          528 c          421 g          450 t
ORIGIN      
        1 cgcgccatgc gcagccggaa ataaagggat aacgatgata gtttgagaaa aacagaaaca
       61 acacttttgt ggcaaagcat ggtgccgggt gcctcccggt gaattcagta tcagcacctg
      121 aatccgcgat tatcccatat acctggttgc tgatcgcccc tccgcacagg gggattcacc
      181 atgcagaagt gtttttaata aacagcaaac aaaaaaatca agcattatgc aggctgtttc
      241 tttttatcac cggccacagc aataccacaa tgccgcagac cagcacccca tccgccagca
      301 ccgacatgat tctgctggtg aaatccacca tcaccaccag aaacagcagg agtgcagcca
      361 cagccaggcg cagttttacc gtcacaggtg attctccaga cgaagaccca gaacaccggc
      421 aatctcttcc agcaccttgc gctcttccgg ctcaatttcg ccgtctgcct ccgcaatggc
      481 caccgccaca tccagcacat cttccgcttc acgcgtatcg tgtttcacat cctcaatctc
      541 acgtaacgcc gcacgacgac cagttttaaa gttcgtatcc agctgaccga taatggttgc
      601 gctaatcgca ttaatttctg acgtaaacgc ggacagcgca ggctgattac gcagtacctg
      661 ttcgatcttc gctttctcgg atgcctcaca ttcaccatct gcacaggcca ccaggtatgc
      721 ggcgttaatc accacctgtg ccagatcgcg tttttcaaac tttttaattt ccgttgccgc
      781 tctgcgggtt ttctttttga agattccaaa catcgtgacg ttcctttggg tgggtgagcc
      841 aacgcccggg agcgatctgc ccacagagaa agtcacactg accactccgt aagctcaccc
      901 ccgaaaggct ctgtggttgg tatgcgccgg gcgtggcgca gatacaaaaa aggcccgccg
      961 aagcgagcct ggaaaataag tgtggcgcgt tgtagtggag tcgaaccact gaccgattgc
     1021 ttagaaggca attgctctgt ccggctgagc taacaacgca gaataccgat aatggaccgc
     1081 catcggggac ccggccccgc accaacaacc ctgttatggt gtcgtctgct cttcctgata
     1141 agctaatggc ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta
     1201 tgagtcgctc gctctcacca ctgagctaaa gggccgagcc aaaaaataat aatcagatga
     1261 aaatcaataa tcaagccctt gcctggatac atatctgtct ggcgggaagc cataatagcg
     1321 gtgaaataca gaaataaagt aggaccggct tgaataacca catttttctg ctacagcctg
     1381 tccatatcca taccgggaac ataacatatt tacagcaaca cgcatccgct cttccagcaa
     1441 caagcgactg aacatgcccc cttcattttt cagttttgtc tttaacaaac tctcactcat
     1501 atgcaactgt agagcaatcg caccaagcgt ccagcttgct gatatatctg tctgaattat
     1561 cgccctgact ttggcactta tgctggataa acatccactt aaaaacaatg acatccgttc
     1621 atctgtttca aacagagaca ggcaggccat cataagaaac atatccgtga cctctccgga
     1681 aaatccctgg ctggtaatta aagccgcagc caacgcagga ttgttgggtt ccagcaacag
     1741 gtaaagcgga atgtcagtca gacgagtcct cgtcagctta tgctgacttt ccagatattg
     1801 acttacgacg gattcgctta tatcgacaat tttaactttg ccataatgca taaggaaaag
     1861 ctccctgatg catttggtgg ccagaacgac tgagcctggc ttaagt
//
LOCUS       DAZFTN010000306         1895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFTN010000306 DAZFTN010000000
VERSION     DAZFTN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            136..390
                     /gene="bssS"
                     /locus_tag="ACHKV2_005358"
     CDS             136..390
                     /gene="bssS"
                     /locus_tag="ACHKV2_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO6943029.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            505..1623
                     /gene="solA"
                     /locus_tag="ACHKV2_005359"
     CDS             505..1623
                     /gene="solA"
                     /locus_tag="ACHKV2_005359"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO6943030.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            1671..1784
                     /locus_tag="ACHKV2_005360"
     CDS             1671..1784
                     /locus_tag="ACHKV2_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO6943031.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          490 a          460 c          446 g          499 t
ORIGIN      
        1 aagcagaaat ttatctgttc ccccctttaa aaattcggtg attaccctta tttattggta
       61 tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg
      121 aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg
      181 tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc
      241 ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat
      301 gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat
      361 ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc
      421 tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta
      481 aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc
      541 gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct tatgaccgac
      601 gcccatatgc caccacatca acacggcagc caccacggcg atacgcgatt aattcgacat
      661 gcttatggtg aaggcgaaaa gtatgtcccg ctggtcctcc gcgcacaaac gctgtgggat
      721 gacctctccc gccacaacga agatgatccc atttttgtac gctctggtgt cattaacctt
      781 ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc
      841 aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg
      901 gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga actggcgatt
      961 aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc
     1021 accgcaattc gtcatgacga tgatggcgta actattgaaa cggctgacgg tgagtatcag
     1081 gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagctgcct
     1141 gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta tagcgtgaag
     1201 aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta tggttttccg
     1261 gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg
     1321 gatgaacgtg ttccgtttgc ggaagtggtc agcgatggtt cggaagcctt cccgttcttg
     1381 cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac ctatgataat
     1441 tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt
     1501 accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat
     1561 tttgcgcaag acaaaaaaag cgattttgat ttgacgccat tcagcctttc ccgcttccaa
     1621 taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc
     1681 tgttgcacta tctcatcaat aatattcgcg agcatctgat gctatatctt tttctctggg
     1741 gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc gcactgacat
     1801 aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc
     1861 tgcttctaca gcactcacca gacatctctg atgct
//
LOCUS       DAZFTN010000307         1879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFTN010000307 DAZFTN010000000
VERSION     DAZFTN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..377
                     /gene="espZ"
                     /locus_tag="ACHKV2_005361"
                     /gene_synonym="sepZ"
     CDS             78..377
                     /gene="espZ"
                     /locus_tag="ACHKV2_005361"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFO6943032.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
     gene            461..838
                     /gene="sctI"
                     /locus_tag="ACHKV2_005362"
     CDS             461..838
                     /gene="sctI"
                     /locus_tag="ACHKV2_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO6943033.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            841..1413
                     /gene="escJ"
                     /locus_tag="ACHKV2_005363"
     CDS             841..1413
                     /gene="escJ"
                     /locus_tag="ACHKV2_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO6943034.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1419..1874
                     /gene="sepD"
                     /locus_tag="ACHKV2_005364"
     CDS             1419..1874
                     /gene="sepD"
                     /locus_tag="ACHKV2_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO6943035.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
BASE COUNT          630 a          364 c          372 g          513 t
ORIGIN      
        1 tataatagtt gcctatggga taatttggtt atttataacc aggtaaatgt ctaaattaga
       61 caaaaggaga aagatcaatg gaagcagcaa atttaagtcc ttctggtgca gtaatgccgc
      121 tggcgacctc actcagtgga aataactcag tggatgagaa gacaggagtg attaaaccag
      181 aaaatggaac aaatcgcacc gttagagtta tagccggatt agcacttacc actacggctc
      241 tggcagctct aggtacaggt attgcagcgg catgctcgga gacgagcagc acagaatact
      301 tagccctggg tattacttct ggcgtactag gtactcttac tgcggttggc ggtgcattag
      361 cgatgaaata tgcctaaccg gcccatcgca taaataaaag atagaggtaa tggatgtatt
      421 atgcccttgc ctctttcata aaaagaggct aacagtaaat atgaacaaca ttaatcaatc
      481 ggaaaacatt aatattcaat taaataaagc gccccaaaca aatttcgttg atgagcatac
      541 ctcgttagcc tcagccccct ctgcggcagg tgcggctcag tttcttgatc aattactccc
      601 taaaacagcg ggagtgtctt ctccagaaca agtgttgatt gaagaaatta aaaagagaca
      661 tcttgcaaca atgaacagcg atctcagttt cgatgctcta tctgcaggtg ggctctcgcc
      721 agaagacgtg ctcaccttac aaaagaatgt gctcaacgca aacgtcaacg ttgatgtcgt
      781 atctaagtta gcaagcctcc tttcaacatc ggttacaaaa ttagtttcga tgcaataaaa
      841 atgaaaaaac acattaaaaa ccttttttta ttggctgcta tttgtttgac agtggcatgt
      901 aaagagcaat tgtatacagg attaacagaa aaagaagcta atcagatgca agcactgttg
      961 ctatccaatg atgtcaatgt ttccaaagaa atggacaaaa gtggaaatat gacactcagt
     1021 gtggaaaaag aggattttgt tcgcgcaata acgattttaa ataataacgg cttcccgaaa
     1081 aagaaatttg cagatatcga ggttattttc ccacctagcc agttagtggc atcgccatca
     1141 caagaaaatg caaaaatcaa ctatctcaaa gaacaagaca tcgaaaggtt gctaagtaaa
     1201 atacctggtg ttatcgattg cagcgtttca ttaaatgtta acaataacga aagtcaaccc
     1261 tcctcagcag ccgttttagt catcagctcg ccagaggtta acttagcacc atcggtcatt
     1321 cagataaaaa acctagttaa gaatagtgtt gacgatctca aattagaaaa tattagtgtt
     1381 gttatcaagt catcctcagg acaggacggg taatatgtat gaacaataat aatggcatag
     1441 caaagaatga ttgtgactgg ctaacggccc ttgattttgt aaaggatgtc aacggttcac
     1501 caacgcatct cactttttat atctaccaaa aaaatgcttt cttgcacgat tttggaaact
     1561 actgggttct atatattgag cttagtggtg attttcgtca ggttccaaca gatacattta
     1621 tcagactctg caatatcctg gccgttagta acgaatataa acaaatgggt attttcctgt
     1681 ctaataagaa gtggtatctt tgccaaattt ttcataaaga taataatcat cgcgcaaaca
     1741 tgtcaaaagc aataatgcaa catactctcg cctctttgct cgataagcaa tttgataaac
     1801 tagagcaatt atcttcatcg gatacaatga tgccgccaac acacttgttt tctgatatag
     1861 gacgaattgt gtaatgaaa
//
LOCUS       DAZFTN010000308         1869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFTN010000308 DAZFTN010000000
VERSION     DAZFTN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..191
                     /locus_tag="ACHKV2_005365"
     CDS             90..191
                     /locus_tag="ACHKV2_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943036.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            188..643
                     /gene="ybcN"
                     /locus_tag="ACHKV2_005366"
     CDS             188..643
                     /gene="ybcN"
                     /locus_tag="ACHKV2_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO6943037.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            643..813
                     /locus_tag="ACHKV2_005367"
     CDS             643..813
                     /locus_tag="ACHKV2_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6943038.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            806..1096
                     /locus_tag="ACHKV2_005368"
     CDS             806..1096
                     /locus_tag="ACHKV2_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6943039.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1093..1455
                     /gene="rusA"
                     /locus_tag="ACHKV2_005369"
     CDS             1093..1455
                     /gene="rusA"
                     /locus_tag="ACHKV2_005369"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6943040.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1452..1592
                     /locus_tag="ACHKV2_005370"
     CDS             1452..1592
                     /locus_tag="ACHKV2_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6943041.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1678..>1869
                     /locus_tag="ACHKV2_005371"
     CDS             1678..>1869
                     /locus_tag="ACHKV2_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6943042.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNN"
BASE COUNT          477 a          455 c          485 g          452 t
ORIGIN      
        1 gacttttgat tttcaataat caacttgtca taattaagtc accggagttt gaactcctcc
       61 ggtgacttct gcgctaaacg gggacgttta tgcgcacata caatctaaac tatcttctcc
      121 cgtcacagat gcagaaatgc acctgcgatt ttctttatcc agcgtttgac ctctgcggag
      181 gtgaagcgtg aacctcccac aagacggcat caaactgcat cgcggtaact tcaccgctat
      241 cggtcagcag ctccagcctt atttggaaga cggcaagtgc tttcgcatgg tgcttaaacc
      301 gtggcgcgag agacgcagtc tttcccagaa tgcacttagc cacatgtggt acagcgaaat
      361 cagtgaatac cttatccgca gggggaaatc gttcgctacc gcagcatggg taaaagatgc
      421 tctcaaacac acatacctcg gttatgaaac caaggacctg gttgatgtcg taaccggcga
      481 aatcactact atccagtcgt tacgccatac ctccgatctt gataccggag agatgtatgt
      541 cttcctgtgt aaggttgaag cctgggcgat gaatattggc tgccacctga ctattccgca
      601 gagctgcgag ttccagctgc tgcgcgacaa gcaggaggcg taatggctac accgcttatt
      661 cgtgtcatga acggacacat ctacagagta ccaaatcgtc gtaagcgtaa gcctgagcta
      721 aaaccatccg aaataccaac actgctcgga tataccgcca gcctggttga taaaaaatgg
      781 ttgcgactgg cagcaaggag gaatcatggc tgatttgaga aaagcagcgc gtggtcgcga
      841 atgccaggta agaatccctg gcgtatgcaa tggcaaccct gaaacgtctg tactggcaca
      901 tatccggctg gctggactgt gtggcaccgg tattaaaccg ccagacctta ttgccaccat
      961 tgcctgttct gcctgtcacg acgaaatcga ccgccgcacg cattttgtcg atgctgagta
     1021 cgcaaaagaa tgcgcgctgg aaggtatggc gagaacgcag gttatctggc tgaaagaggg
     1081 ggtaatcaag gcgtgaatac ctacagcatc acattaccct ggcctccgag caataatcgc
     1141 tattaccgcc ataatcgcgg gcgcacgcac gtcagcgcag aggggcaggc ataccgcgat
     1201 aacgtcgccc gaatcattaa aaacgcaatg ctggatatcg gcctggctat gcctgtgaaa
     1261 atccgcattg agtgccacat gccggatcgc cgtcgccgtg acctggataa tctgcaaaaa
     1321 gccgcttttg acgcactcac taaagcaggt ttctggctgg atgatgctca ggtcgttgat
     1381 taccgcgttg tgaagatgcc tgttaccaaa ggtgggaggc tggaactgac catcaccgaa
     1441 atggggaatg aatgatgttt gagtttaata tggcagaact tcttcgccac cgctgggggc
     1501 gtctgcgctt atatcgtttc cccggttctg ttttgaccga ttaccgaata ctgaagaatt
     1561 acgccaaaac cctgacagga gcaggagtat gaagtcagag ataacaatca actaatactg
     1621 ttttgttgat ttttgcttgt aattggcgtt ctggtctgat ttttgtggag taagttgatg
     1681 cgtgatattc agatggttct tgagcgttgg ggagcgtggg cggctaataa tcatgaagat
     1741 gtgacctggt cgtccattgc cgccggtttt aagggactga tccccgaaaa agtaaaatca
     1801 cgcccgcagt gctgtgacga tgatgcgatg gtgatatgcg ggtgtatagc ccgcctttac
     1861 cggaacaat
//
LOCUS       DAZFTN010000309         1848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFTN010000309 DAZFTN010000000
VERSION     DAZFTN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..272
                     /locus_tag="ACHKV2_005372"
     CDS             <1..272
                     /locus_tag="ACHKV2_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6943043.1"
                     /translation="DHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKI
                     DLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(365..724)
                     /locus_tag="ACHKV2_005373"
     CDS             complement(365..724)
                     /locus_tag="ACHKV2_005373"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6943044.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1410..1700
                     /locus_tag="ACHKV2_005374"
     CDS             1410..1700
                     /locus_tag="ACHKV2_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6943045.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1724..>1848
                     /locus_tag="ACHKV2_005375"
     CDS             1724..>1848
                     /locus_tag="ACHKV2_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6943046.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNR"
BASE COUNT          555 a          345 c          371 g          577 t
ORIGIN      
        1 ctgaccatgg agaatctctg ggagaaaatg gattatactt acatggaaca ccatataatg
       61 ttgctccagc tgagcaaacg catgtgcctc ttattacctg gatgtctccg gggttcgttt
      121 cctctaaaaa aattgatttg aattgtttgt cagaacatgc tctaaacaga acagtaagtc
      181 atgataatat attttcatcg ttactggggt tatggaatgt aaatacttca gtttataata
      241 aagaagatga tattatatcc tcctgcagat agatactgac atttttctga ggataagagg
      301 tgaatacctc ttatccttaa gtaaatatta accaggtaac tgtgaataat ttagatgttg
      361 ggcgttatga aagtaataaa actccccata taatgatagc aactaaaatc gtaatcaaca
      421 ctgcggctga acccatatct tttgctctgc ctgataattc gtgatgttct gaaccaattc
      481 tgtcaacaac tgcttcaatt gcactgttaa ttatttcaac aataattacc atcattgccg
      541 aaccaatgag taatactcga gtaacagagt caacatccag ccaaaaggat attatcagtg
      601 ctgacagaac cagaatgcac tcctgacgaa aggctgcttc atagcgccat gccgcacata
      661 agcctttcca tgaataaccc gctgctttaa taattctggt aacaccagtg gtcgtattag
      721 ccataaatac ctcttattta attatatgtc tgaatatgca ttataaaata tcagttaaca
      781 ttaacaggat tttgtaataa gccacattta ttctttcggg aattaaaaaa cataacgaaa
      841 ttttgcctca ttctgcattt cattgaaata gtatgtttct catcgaaagc aagtttgtca
      901 atataaattt gtaacagaaa tgactttccg gggataaaca aaactgttca tggcaatggc
      961 tttctctgtc gccaacatgg atacattgtt ccggataaag cactgatgtc attgactgac
     1021 gcagactatc aggccatctg atgctttctt ttccttcacc ggtattgatg atatgcctta
     1081 tgaaggggga cttattcctg taacccaatc acaggcactg tgattactgg tttatgattg
     1141 atgctgattt gagcagatat gcacagcagt tgtggattca ggggatattt tctgccagat
     1201 attcatacca gattgatgac ctctttgctc agatgctctt cgttctgagc cagccaggat
     1261 tttgtggcca catacattgt tcgccgatgg ggaatcgaga caggcgattg gctgtgatgc
     1321 gattttgccg ctaactgagg atgagtccgc ccctcccggg gaattcctgt aagttaagcc
     1381 gtacctgttg attagcacta aactgggtaa tgaatttatc tgccagtatg aatgatgccg
     1441 gggacggttt tgaactcctg atgtcgggta atatgcagga ttttaatatt tgttgtcgtc
     1501 gggataatca ttatcctgaa cataaactga tacctttatc agagttattt gacgttaatt
     1561 atggactgaa tcttgagctg aataaactgg agaaagattc ttcaggaatt aattttgttt
     1621 caagaacgtc caaaaataat ggcgtctccg ccagggttaa attaaaagat ggtattagtc
     1681 catgccagca gctgtgttaa ctgtagcagc aggaggttct gttatggaat cgtttgtaca
     1741 ggattcacca ttttatagtg gaagagatct ttattggtta cgtcctaaag ttgaactgac
     1801 tctggaagag aaattgtact actgctcttg tataagaaga aacagaca
//
LOCUS       DAZFTN010000310         1844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFTN010000310 DAZFTN010000000
VERSION     DAZFTN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..110)
                     /locus_tag="ACHKV2_005376"
     CDS             complement(<1..110)
                     /locus_tag="ACHKV2_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943047.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYG"
     gene            complement(94..>1844)
                     /locus_tag="ACHKV2_005377"
     CDS             complement(94..>1844)
                     /locus_tag="ACHKV2_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6943048.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          356 a          569 c          448 g          471 t
ORIGIN      
        1 ccataaaata cgatgtcaga atttgaattg ttatcaaacc caaggtaaac aacaaataaa
       61 caaagggtaa ttaaaaaaat gacaagtttg cctctaataa tttgaagcat ctgaactccc
      121 ttgctttgct tttattggat tatctgcttt tataccatct aacccgaaga gttgcccaat
      181 gttatatttt tcaatacatg catcgctaca tttggcagaa aaatcgccac aagcaatacc
      241 gacagtgtta tttttaagat ccatgattct ttctttagct ggctgacctt gtctatcccc
      301 agccgcctca tgatttttac ctatcacgtc agctattgtt gatccaaatg tcttagtcat
      361 tcgacacatc aagtaacaat atctcattgc atcggcggcc ccattatgag ctccgccaac
      421 aatagcatcc atatcataac cgaactggta agcactgtta gtaatgctgt ttgcaacaat
      481 caatccaggt acaccagata cggcataaat tagtccacct gatgtcatat tctcaatcgc
      541 atctaacccc attgggtcta tcacttgtat gggattcaac ggatactgat acatattcca
      601 tccacctctc aacccaatcg ggtccggggt gatataccgc ccctgcaacg gatcgtagta
      661 ccggtgacgg ttatagtaca gccccgactc ctcatcatgc tgctggcccg gcagacggta
      721 cggctggtgc aggtgatgcg ggttctcctc attaagctgg ttgccccatt catcatattc
      781 cgcgctccac gccgtattgc cgtcttcgct gataagcgcc agcggcagtc cccggtggtc
      841 acagtggtac agatgcagcg tgcgcgccgg cgtgtattcc ggctccacct gtttttccag
      901 ttgctccacc gtcagtccgc actgcgcaag ccacgcccgg ctttcactgc tcacgcggtc
      961 tgcccggatt tcttcctcca gcctgtccag catcctcacc agttctgccg ggaacaccac
     1021 accgtgaccg tcctcactcc cttcctgctg gagtttctcc gccaggctgc ggcgctgcgc
     1081 tttctcccgc tcgccgttct ccgtttcgat gcggatgagc ggtgtgaagc tccccggccg
     1141 gtataccgtc tggatacgtg tggtgccggt ctgtaccgtc gtcagcctgt cgccgtccca
     1201 cccgtaccag gtcacctccg gtttacgcga cagcgacatc caccccgtca ggtcacgctc
     1261 ccgccgccac acccgtttcc ccgtccggcg gcccagcggg tcatagaggt agcggctctc
     1321 gaccagcggc tcgccgtatt gtatccgcgt gtagaacacc aggcggtgct ggttgtcgta
     1381 atggtagtgg tgggtccgct cgtcgtgcat ccggataacc ccttccggaa tgcggtccgt
     1441 cttctccgtc agcctgccgt attcatcata gcggtagaca tagtgcgcat cttccgcgat
     1501 gcggttatcc ggccacgccg tgagcgtgct gtccggatgc agttccgggt ccggcagccg
     1561 gtttcctgcc gggtccgttg catacgggat atcgatatcc aggttcgctg ccgtggtgtg
     1621 gacgcccgtc agccttcccg tgtcgctgta acggtactcc cggctctcct gcgggccgct
     1681 gatgcggatt agctgtccgt tgtcgttcca gtcgtagtca cagtcaagct gcgggaggtt
     1741 caggtgcctg ctctgtagct gccctgtgag ggtgtacgcc gtgacctgtt cgtaccccgc
     1801 tgtactgccc gccccgccga agctgcgggc cgtctcacgg tgca
//
LOCUS       DAZFTN010000311         1824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFTN010000311 DAZFTN010000000
VERSION     DAZFTN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1466
                     /locus_tag="ACHKV2_005378"
     CDS             <1..1466
                     /locus_tag="ACHKV2_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6943049.1"
                     /translation="NNLNSKECLDDSFINFAKAYMLHVHSFNKSKTKHSTLSMLKIVE
                     FVLLKINMEANVNYCNNSIYDECIRIASEKYSKAHAFAIGKELEKLSSFLNDNRMTNS
                     FYLFWVNPIRYRITQSWTGYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTT
                     SVLALLMCAPSRISEILALPADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMI
                     PVAKKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKENIPPDLFWYDREKKIKYSNA
                     LCLLTEGQLNQNKKEMSDKLFRPTTNFFKTDIIDSDYIKGYFNVFKRHGYINEDGSPY
                     LLRTHQLRHLLNTFAQINGMDEFSIARWSGRKLISQNVSYDHRSHLQMSKAIREKKLS
                     VCVNEHRIKDIPVVDLNEFDSLSSGAVLVSKHGYCKHSYAFKPCDNYPIKNSGLDNET
                     ISNIHDKILKRTLYDKNDGNINADKWYEFHKKIKKGE"
     gene            1467..>1824
                     /locus_tag="ACHKV2_005379"
     CDS             1467..>1824
                     /locus_tag="ACHKV2_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943050.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQIT"
BASE COUNT          675 a          258 c          338 g          553 t
ORIGIN      
        1 aaaacaacct aaacagcaag gagtgcttag atgatagctt tattaatttt gctaaagcat
       61 atatgttgca tgtgcattca ttcaataaat ctaagacgaa acactcaaca ttatcaatgt
      121 tgaagattgt cgaattcgtt ttacttaaaa tcaatatgga agctaatgta aactattgca
      181 acaattcaat ctatgatgaa tgcataagga tagcctctga aaagtattct aaagcacatg
      241 catttgctat tgggaaagaa cttgagaaat tgagttcatt tttaaatgat aataggatga
      301 ctaactcatt ttatttattt tgggtaaatc caattaggta taggattact cagtcttgga
      361 ctggttatga ttcttcactg gaaggccatt ctagattgcc tgatatcaaa tcagttattg
      421 cgattgctga gattttttca aagcgggatg aacaattatc gtcaagagat atatttacta
      481 catctgtgct tgctttactt atgtgtgcac cgagtaggat atcggaaatt ttagctttgc
      541 cagcggattg tgaaatcaca gaatgtgatg gaaagggcat tcaaagatac ggtttaagat
      601 ttttttcagc aaaagggtat gaaggcaata taaaatggat tccaacttta atgatacctg
      661 tagctaaaaa ggctattagc agattaaaag aattatcaag tcaagcgagg ttattggctg
      721 ctgaaattca aaagaattac tctaattcaa cgaagggaac ccttaaagaa aatatacctc
      781 ctgatctctt ttggtatgat agagagaaga aaatcaaata ttctaatgcg ctttgcttgt
      841 taactgaagg acagttaaat caaaataaaa aggaaatgtc agataaatta ttcagaccta
      901 caacgaattt ttttaaaact gatatcattg attctgatta tataaaaggg tattttaatg
      961 tttttaaaag acatggttat ataaatgaag atggtagccc atatttgcta agaacacatc
     1021 aactaaggca tcttctcaac acatttgctc aaataaatgg tatggatgaa tttagtattg
     1081 ctcgctggtc tggacgtaag cttatttctc aaaatgtttc ttatgaccac agatcgcatc
     1141 ttcaaatgtc taaagcaata agagaaaaaa agttatcagt atgtgttaat gagcacagaa
     1201 taaaggatat tccagtagtg gatcttaatg agtttgactc acttagtagt ggtgcagtac
     1261 ttgtatcaaa acatggctac tgcaagcact catatgcgtt taagccgtgt gataattatc
     1321 caattaagaa ctctggttta gataacgaaa cgatttcaaa tatccacgat aaaattttaa
     1381 aaagaacact gtatgataaa aatgatggga acataaatgc tgataaatgg tatgaattcc
     1441 ataaaaaaat aaaaaaagga gaataaatgg ctaagcatat aaatggttat catgagaaga
     1501 agattgtaga tgttattaac acatggtcta tagatgaaaa actaacctgg aatgctttgt
     1561 gcgataggtt agtcagggtt attgggaaaa gaccatcaag acaatcctta agtagtcatg
     1621 taaggattgc ggaaagcttc aatgtgaaaa aaaccacaat caaatctggt gtaatacaca
     1681 ctgtaaaacc tgctaattta aaagtggctt ctcaacgaat taaaagactt gaagcagaaa
     1741 atgaagcttt gaaggcatta aatgaacgct atttagagca gttcaaagta tggctttata
     1801 atgctcacct taaacaaata acga
//
LOCUS       DAZFTN010000312         1822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFTN010000312 DAZFTN010000000
VERSION     DAZFTN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..351
                     /gene="ydfC"
                     /locus_tag="ACHKV2_005380"
     CDS             133..351
                     /gene="ydfC"
                     /locus_tag="ACHKV2_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6943051.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            374..781
                     /locus_tag="ACHKV2_005381"
     CDS             374..781
                     /locus_tag="ACHKV2_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943052.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(759..992)
                     /locus_tag="ACHKV2_005382"
     CDS             complement(759..992)
                     /locus_tag="ACHKV2_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943053.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            1551..1739
                     /gene="dicB"
                     /locus_tag="ACHKV2_005383"
     CDS             1551..1739
                     /gene="dicB"
                     /locus_tag="ACHKV2_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6943054.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            1727..>1822
                     /locus_tag="ACHKV2_005384"
     CDS             1727..>1822
                     /locus_tag="ACHKV2_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6943055.1"
                     /translation="MLAMNTAFALVLTVFLVSGEPVDIAVSVHRTM"
BASE COUNT          522 a          387 c          422 g          491 t
ORIGIN      
        1 gccaccacat tctgggcaag aaaacttgat tgtattcata accaatttcc tctcgagtaa
       61 cagacccctc agaggatacc acctcgcctg acgtggttaa aaagcaggca acgctaacca
      121 caaggagccg acatgcagaa acgagacccc gtcatcatcg cgccagacta taccgatgat
      181 gaactttatg agtggatgca ccagaaaatt aaggcggtgc aggacctgaa atgggccaat
      241 gaagccaggg ctaagcaggc tgaaaatctg tccgctctgg agcaggatat caccaatctg
      301 gaaaaagcag cggcattaag cattgccaga atgattacat acccacgtta atagctaacc
      361 aacgaggcta ataatggaat ttaaagattt accaacgcca ttacaggaaa tggcatcgaa
      421 tatagttcgt tcacaactgg ctactcttga ccttagtacc gcagaaaaag aaaccatcga
      481 taatatggtt cgtaatgtgc gcaatgcttt ttctgggcta tatggttctg ataatcaaaa
      541 gcaggaaagc gatgttaata aacgggtaat ttctgtttgc gtgaatggcc atgttctttc
      601 atcaatcaaa acagaaacgg cgacagtctt cgattgcctt tgcattgtac agagccttgt
      661 tgatgccctg tttcgttcag tgaatttaga aaatgatgca aatctgcgag ggcgcataat
      721 agcacatcca tatgcacata ctttaggctc tgtggatatc aaagatccca caaatcttta
      781 atgaaatagt taacgcgaat tgtacttgct ctttcggttg ctttcagaat acgcgttgaa
      841 actgctggcg gtaatttggt attccattta ttaaaatcat gcccgggaaa gtactcttcg
      901 aaaatacttt taactgcaga ctcgcctatt gaaatgctgc ttaccatgcg attttgataa
      961 aggcatttag caataagagt tgattttaac attcaccctc ctgagggttg gtaattaagg
     1021 agttctccac gggtgaagtg gagtgcgtgc gccggacacg ggtgaacatc cggcactgac
     1081 agtttactga aaggatattt ctctgaaaag tcagagcata acgcgaaagc gcacggcgag
     1141 gttgctggtt catagatagc ctgtcgttaa attttcgtcg accgtgcgct tccggttgtg
     1201 gcactccgcg aaatggcgca gcggtaagta tggcggggtt attccttccc cgttgaggac
     1261 accgggttgt caggttgacc atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta
     1321 tcaattttct ggtgacgttt ggcggcatct gtttgcccat gaactgatgt ccgccctttt
     1381 taaagtgaat tttgtgatgc agtgaatgcg gctaagcgca cgcggaacag ttaaagctaa
     1441 aaacagtgtt atgggtggat tctctgtatc cggcgttaat tgttaactgg ttaacgtcac
     1501 ctggaggcac caggcaccgc atcacaaaat tcattgttga ggacgcgata atggaaacgt
     1561 tattaccaaa cgttaatacg tctgaaggtt gttttgaaat tggtgtcact atcagtaacc
     1621 ctgtatttac tgaagatgcc attaacaaga gaaaacacga acgggagtta ttaaataaaa
     1681 tatgcattct ttcaatgctg gcccgtttac gtccgataca aaaaggatgc tggcaatgaa
     1741 tacagcattt gcacttgttc tgacagtttt tcttgtttcc ggagagccag ttgatattgc
     1801 agtcagtgtt cacaggacaa tg
//
LOCUS       DAZFTN010000313         1803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFTN010000313 DAZFTN010000000
VERSION     DAZFTN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..642)
                     /gene="nleH1-2"
                     /locus_tag="ACHKV2_005385"
     CDS             complement(<1..642)
                     /gene="nleH1-2"
                     /locus_tag="ACHKV2_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO6943056.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKI"
     gene            complement(749..820)
                     /locus_tag="ACHKV2_005386"
                     /pseudo
     CDS             complement(749..820)
                     /locus_tag="ACHKV2_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            814..1803
                     /locus_tag="ACHKV2_005387"
                     /pseudo
     CDS             814..1803
                     /locus_tag="ACHKV2_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          539 a          437 c          379 g          448 t
ORIGIN      
        1 tattttattc attctaatac caataacatt tccattatca ttatatattt tctctgcact
       61 cccggaacca taatactgat tgaaacaacg aacttcgctt gtcacctctt catggctttg
      121 agatatagta aacatcttca acacttttgt tgtatcttcc atatcttcat acacgacagc
      181 attaccacct ttaccaataa cattacctgg cacgggtgat ttattgttgc taaccctcgg
      241 caactctgat tgagtataat ccgctggagg taactccggc tgcgcataat ccactggagg
      301 taaatcaggt ctgttcgaat gaacagagcc tctttctata gtcgtgctca ctggcgatgt
      361 attcagcata gcctcaattt ttctgctaat ctctccctta ggccatccca acctgtgcaa
      421 cagattagta aaacaaccac tatgactttc tcttgtaacg caaaacttat tatcagtgac
      481 cacaacacga tatgttctgt tgccaaccgt tacttgcgtc ccgctatcag agtgaacagc
      541 agcatccctt acagaggata aaacacgatt atcaggcgaa gtcaggtttc tggttaaaga
      601 attccatgaa catcccaaat ttatagaaga gggcgataac atacatttca accttcaaaa
      661 taaacctatc taattattcc taacagacat cccccataca tgacaacaaa aaccggagcc
      721 ggactccggt ttttgtgaag ctatcgggtt acttcatttc gccaatattt tcccacttcc
      781 cgtcagcacg caggatttgc agcggtctta ctacacactg acccgaggac gaaaaaagag
      841 ttcgggctgg gaagagacag aagagtagca atttcagaag cattcacacc aacattgagc
      901 ttttcagcga aagtgggcac gaatcattgc tggacagggt taaaggcact gactcaatca
      961 ctcttcatac gtggcttgat cgatatgaaa caatcctcag ctagaggggg agcaagccga
     1021 aaacattact cgactacgcc agcaaaatca gggcaattcg aagaaaattg ccggacaaac
     1081 cgctcactga catatcaacg aaagaggtgg cagcaatgct aaacacctac gtcgcagaag
     1141 gtaaagcggt ttccgcaaga gtaatcaggt caacccttgt tgacgttttt cgaggggcaa
     1201 tagccgaggg gcatgtggca acgaatccag taacaacaac ccgtgcagca aagtcagaag
     1261 taaggcgctc aaggctgaca gctaatgagt atgtcgctat ttaccatgct gctgagcacc
     1321 tccccatctg gctgaggctg tcaatggatt tagctgtcgt tacagggcag agagtgggcg
     1381 atttgtgcag aatgaagtgg tcagacataa acgatggtca tcttcacatt ggacagagta
     1441 aaacaggagc caaaattgcc attccgctgg ctctaaccat tgacgcactc gacatctcac
     1501 tggttgatac actacagaaa tgcagggagg ccagcagcag tgaaacaata atcgcatcaa
     1561 cctatcacga accactttct ccagccacag tatcacggta tttaacaaag gcgcgaaatg
     1621 catccgggat ctcgtttgat ggagacccac cgacatttca tgaactacgt agtctgtccg
     1681 cgaggctata tcggaaccag attggctaca agtttgcaca acgtcttctt ggacataaat
     1741 ctgattcaat ggcggcgcat tatagggaca gtcgcgggcg ggagtgggac aaaattgaaa
     1801 tcg
//
LOCUS       DAZFTN010000314         1802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFTN010000314 DAZFTN010000000
VERSION     DAZFTN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1524
                     /locus_tag="ACHKV2_005388"
     CDS             <1..1524
                     /locus_tag="ACHKV2_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO6943057.1"
                     /translation="TSLQLSYYEKNIREKQISLNEYEEAFKKSDFKILLGRLTSWSML
                     YLKQHLRRNVSTRSSFSAETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIID
                     EASQQDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSV
                     CMLFRNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNH
                     TRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGREC
                     DEIVFSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIA
                     ALIRYIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACL
                     LRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTP
                     LGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT          517 a          330 c          415 g          540 t
ORIGIN      
        1 acttctcttc agctttctta ttatgaaaaa aatatccggg aaaaacaaat ttctctgaat
       61 gaatatgagg aagcatttaa aaaatctgat tttaaaattt tactggggcg cctgacatca
      121 tggtctatgc tttatttaaa gcaacatctg cgaagaaatg tttcaacaag aagttcattc
      181 tcggcagaga catacaggga tgaatttgac cgttttataa agcgttttcc gattattggt
      241 agcagtacgc attcgattat taactctatc ggcaaggggg cccttcttga ttatgttatt
      301 attgatgaag cctcgcagca ggatattgtt ccgggcattc tgggactggg atgtgcccgt
      361 aatgtaatcg ttgtagggga tcgtaagcaa cttccacatg ttcctgtatt actcccgaac
      421 agcccatctc caccggcaga atactataat tgtgagaaat atagtctact tgattcggtt
      481 tgtatgttgt tcaggaacat ggtgccagta acccttctta aagagcatta tcgttgtcat
      541 cctaaaatca ttcaattttg taataagcag ttttatgata atgctctgat accgttaacg
      601 gttgattccg gtgaagcctc attatctctg gtcatcacgg caaaaggaaa ccatacccgt
      661 aatttttcaa atctcaggga gcttgaatct ctggaaggtc attactggga tgaagaaagt
      721 agcaggggat acattgcccc atataatgca caggttaatc tggctgaaaa agtgctgcct
      781 gctgattttg tgaaatcgac ggtgcataaa tttcagggac gggagtgcga tgaaattgtg
      841 ttttccactg tgctggataa gaagcgcagc agccagcaca gtcggaacat cgcgtttgtt
      901 gataatcctg aactggtgaa tgtcgctgtt tcaagggccc gaaataaatt tacgctggtg
      961 actggtaatg atgtatttga acgccacgct ggacatattg cggcgctgat tcgttacatc
     1021 aaatactacg ctgatgacgg agagattttc gagagcccgg tcatatcagc atttgatttg
     1081 ctgtacagtg agtatgacaa atcactggaa cgactgaata gtagattgaa ttcaaatgat
     1141 tcacacttta agtcagaaca gattgtggcc tgcctgttgc gggatatcct gtcgcaggat
     1201 agttatcgga gtatgatgtt tcattcacaa atagcgctga atcagttggt gttgctggag
     1261 cggggggatt ttacacaccg tgaacagtta ttcatgcgta accgggccag ttgtgacttt
     1321 gttgtttact ataaggtcgg gaagactcca ttaggggtta ttgaagtgga tggaggttat
     1381 catctgacca gtgttcaggc tgaacgtgat gaacttaaaa acagtatact gaagaaatgc
     1441 ggcctgccat tgctaagatt acgtactata gacagtgata tagaagggaa gttgggagcg
     1501 tttctgtcag gcctgacagg ctaaaatctg aataaaaaat attgcagcgg gaggtctcaa
     1561 acctgatttg gtcatgtgcc acacacaatt gtacgaaccc catggacaca ggcttctttg
     1621 ttttggttac atcaacgtac gtattcctga tacatataaa aataagtgct attgctcaaa
     1681 gctaacaggg aatgtttctg ttgaataatt ccttagtgaa taaactttat cagggggtaa
     1741 ttctattaca gtgaatactt cctgcaaaat gatatttgaa ttgttcacca tctgcatcat
     1801 at
//
LOCUS       DAZFTN010000315         1764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFTN010000315 DAZFTN010000000
VERSION     DAZFTN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1553
                     /locus_tag="ACHKV2_005389"
     CDS             <1..1553
                     /locus_tag="ACHKV2_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6943058.1"
                     /translation="EYGYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDS
                     TLTAWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVF
                     YTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLT
                     TVQTDTTRIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPA
                     ELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHR
                     GLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRY
                     YDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSS
                     IDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSET
                     KSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACR
                     TWQECWVN"
     gene            1565..1750
                     /locus_tag="ACHKV2_005390"
     CDS             1565..1750
                     /locus_tag="ACHKV2_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943059.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKNLST"
BASE COUNT          459 a          396 c          537 g          372 t
ORIGIN      
        1 gggaatatgg ctacagtgcc acgggcaggc tggagagcgt gcgcaccctt gcatcagacc
       61 tggatatccg catcccgtat gcgaccgacc cggcgggaaa ccggctgccg gacccggagc
      121 tacacccgga cagcacgctc acggcgtggc cggataaccg catcgcggag gatgcgcact
      181 atgtctaccg acacgatgaa tacggcaggc tgacggagaa gacggaccgc atcccggcgg
      241 gtgtgatacg gacggacgac gagcggaccc accactacca ctacgacagc cagcaccgcc
      301 tggtgttcta cacgcggata cagcatggcg agccactggt cgagagccgc tacctctacg
      361 acccgctggg acggcgaatg gcaaaacggg tctggcggcg ggagcgtgac ctgacggggt
      421 ggatgtcgct gtcgcgtaaa ccggaggtga cgtggtatgg ctgggacgga gacaggctga
      481 cgacggtgca gactgacacc acacgtatcc agacggtata cgagccggga agcttcacgc
      541 cgctcatccg ggtcgagaca gagaacggcg agcgggaaaa agcgcagcgg cgcagcctgg
      601 cagagacgct ccagcaggaa gggagtgaga acggccacgg cgtggtgttc ccggctgaac
      661 tggtgcggct gctggacagg ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa
      721 gccgggcgtg gcttgcgcag tgcgggctga cggtggagca actggccaga caggtggagc
      781 cggaatacac accggcgcga aaagttcatt tttaccactg cgaccaccgg ggcctgccgc
      841 tggcgctcat cagcgaagac ggcaatacgg cgtggcgcgg ggagtatgat gaatggggca
      901 accagcttaa tgaagagaac ccgtattacc tgcaccagcc ataccgtctg ccggggcagc
      961 agcatgatga ggaatcaggg ctgtactata accggaaccg gtactatgac ccgctacagg
     1021 ggaggtatat tacacaagac cccattgggc tggcgggggg atggaatctg tataattacc
     1081 cactgaatcc gataataagg atggatcctt tgggtttgta taatttatat caattattat
     1141 atgatgtttg gcatgatgat tcatatggaa catcatcaat tgatattact ggcagtggag
     1201 atctaatatc attaggtggt catgcaggac ttggcgttgc gtttgctaaa aagaaaggtg
     1261 aaatgttatc tgatatttgt atttatgcta cagcatgcgg acatgcagga attggtggtg
     1321 ggataaatgc ggctatcaca tattctgaga ccaagtcttt acctacatcg ggagtcagca
     1381 attcagtagg tgtaacggtt ggcggcggag ttggggggca ttttgcgtat acttatgtag
     1441 tggatgttga taatccagaa tcatcgacag aatctgttgg tatcggtgca ggtgttgacg
     1501 cttcagttat gactctggct tgtagaacgt ggcaagaatg ctgggtcaat taacaggagg
     1561 aaatatgaaa aaatatatat ttttagtttt atttttattg tatggttgtt ctccagtgga
     1621 catagagcca gctagacaag aagttaatat ctttcgtggt ttatatcaga cagggaaata
     1681 cagtgatata tataaaatca cttcaagtga ttttcagact gccacatctg aaaaaaattt
     1741 atcgacataa tgaacgaagc aaaa
//
LOCUS       DAZFTN010000316         1763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFTN010000316 DAZFTN010000000
VERSION     DAZFTN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..249
                     /locus_tag="ACHKV2_005391"
     CDS             <1..249
                     /locus_tag="ACHKV2_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943060.1"
                     /translation="NNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLFINNEQLLEI
                     RECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            242..>1763
                     /locus_tag="ACHKV2_005392"
     CDS             242..>1763
                     /locus_tag="ACHKV2_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6943061.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYIL"
BASE COUNT          518 a          274 c          370 g          601 t
ORIGIN      
        1 aacaatgcac tggtgaccct tcccgcagat gctaattata aagacaaaac gttactcctg
       61 aaggcgattt acagtggatg tacaaatctt ccattttcac tttttattaa taatgaacaa
      121 ttattagaga ttagggagtg ttgtaaacag gcaataaaag taacatttgc agcagagctc
      181 tttgatacac aaaactgtaa caaaaaacaa aataaaaaac catggaagaa ggtgatgttc
      241 aatgtttaac aaagttttgg tagggttaag gagtcatcca gaattaataa ttcttggatt
      301 aatggtgatg attatcgcca tgctcataat tccattgccc acctatttaa tcgacttttt
      361 gattgggctt aacttaacat tggcaatact tgtattttta ggatcattct atgttgatag
      421 gatcttaagc ttttcttcat ttccatcaat cctccttatc acaacgttat ttcgtttggc
      481 gctggcgatc agtaccagtc gacttatact tcttgaggcg gatgctggag aaattataac
      541 gagttttggt gagttcgtta ttggagatag cctggtagtt ggttttgtta ttttctccat
      601 tgttactatt gttcagttta tcgtgattac taaaggttcg gaacgtgtgg ctgaagttgc
      661 tgcccgcttt tcgcttgacg gtatgccagg taaacaaatg agcattgacg ccgatctacg
      721 cgccgggatc attgatgcag atttagcaaa agaacgtcgt agtgtattag aacgagagag
      781 ccagttgtat ggttcctttg atggtgcgat gaaatttatc aaaggtgatg ctattgcaaa
      841 cattattatt atctttgtta atattatcgg tgggctatca gtaggagtag gccaaaatgg
      901 aatggatttc tcaacagcgt tgactgtcta cacaattctt actgtcggtg atggtcttgt
      961 ttctcaaatt ccagctttat taattgctat tagcgcagga tttatcgtta cgcgcgtaaa
     1021 tggcgatagt gataatatgg ggcaaaatat aatgtcccag ttattaagta attcttttgt
     1081 cattgttgtg acatgtgtcc tcgcgctaag tattgggttg cttccaggtt ttccccttct
     1141 agtgttttta tgtttggcag taatcttagg aatttacttt tactttaaat tcaaaaagaa
     1201 agggactgaa gaaacggttg ttgagggaga tattaccgtt gggttggaac cctataatca
     1261 agatgatgat atctcattag gtatcattaa taaacttgat caggttatta ccgaaacggt
     1321 acccttggtc ttgattatga atagtgtaca ggctaaaaaa tacactgaaa tcaatcttgc
     1381 tgataggatt cgtagtcagt tttttattga gtatggtatc cgcattcccg gtattgtcat
     1441 tcgtgagggg gaaggtctta atgatgaaga tgtcatatta atgctaaatg aagtcagagc
     1501 ttcacaattt aaaatttatc atgatcttgt cttgttggtt gaatattcag atgaagtggt
     1561 ttcgactttg atcaagaaac ctgtcattgt aaatagtaat ggtgaacaat attattgggt
     1621 tacgaaaagt gatgctcaaa aattaacaaa aattggctgt tatactcgca cggccatgga
     1681 tgaaatgtat aatcatttat ctgtctgtct ggctcataat attaatgagt actttggtat
     1741 acaagaaaca aaatatattc ttg
//
LOCUS       DAZFTN010000317         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFTN010000317 DAZFTN010000000
VERSION     DAZFTN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..759)
                     /locus_tag="ACHKV2_005393"
     CDS             complement(<1..759)
                     /locus_tag="ACHKV2_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO6943062.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLTEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSA"
     gene            complement(746..>1756)
                     /locus_tag="ACHKV2_005394"
     CDS             complement(746..>1756)
                     /locus_tag="ACHKV2_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO6943063.1"
                     /translation="KVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYLATRALKM
                     ARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFINFLTGVN
                     PAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLPPSPVPPS
                     PSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAA
                     PVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTE
                     AWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYG
                     LW"
BASE COUNT          369 a          583 c          476 g          328 t
ORIGIN      
        1 ggcggatgtc gtccggccga tactgccacc gggccgggta ttcaggcctc ctgaagacag
       61 tgacggcagc gatggcaggg tgatcagggc actgaaaagc cccgcaaaag atgaggccat
      121 gcgggaaggt gctgtcagta atgccgtggc attccctttc agcgccgtga acgtggcagt
      181 aaaggcactg atatcctgta caatccccat gccggaaacg gcattttcca gggcggacac
      241 cttatcactg atggtttcgg tcattgcctg gacatcgtgc attccgtcag agatcaccgt
      301 ccagcccgct tccagggttt caaaaacctg cccgagcgcg gtggtactct tctgctccag
      361 aatggcggcc gtgtcctgtg tgacagcagg tgcggtatca tcacttaccg gcgtgacatt
      421 aatggtgaac tcaaccacgc cctgttcagc ggcgttataa cgactttcaa agctgtttat
      481 cagcacattt aacgtgccgt aatccgggtg aagcagctcc ccggcaccgg gagcacgaag
      541 ggcatcacgc agacgatttc gctgtgtctg aacatcatcc cccatcacaa gtacactgaa
      601 cgtaaattca gtcagtttag gtcccagatc gtctgcccca ccggtttccc gtaacggata
      661 ttcccgacgg acaatgtttc gcccaccacg ttcgcgctgc tcacgccaga cgaggaacgg
      721 cacaccacgg aacgcaccac gaccgtcacc agaggccata attccctccg ttccagacat
      781 tcacatccag tccgtgaccg gacgtgtcat caatatcaat actgcgagcc tgccaccctt
      841 ccggggcaac cagctccaca cgggcagagg ctttttgtac cgcctgtcca ctgctttcat
      901 catcactgcc ccgtaatttc tgccaggctt cagtcagcca tccccccaga tagttaccga
      961 gatagctgcc caccgtagaa ccaatggcaa tccccacagg tccggctgcc gcccccagcg
     1021 caccaccggc aatactgcct gcaagagagc cgaccgcttc tcccttgtct gtggcactgg
     1081 cgctgccatc cagcagaacc ggggctgcca tcgcgccagc ccacagggca cctccaccca
     1141 gccggccggc cagtccaccg gcactgcgga aaaagccgga gaaacgcccc agccccatgc
     1201 ggctaccgac agacgaaagt aacccaccgg cgcgccccat catgcgcccc cagaaccctg
     1261 ccccaccaga agagggagac ggtggcactg gggacggtgg caggggaggc gtgaccggtc
     1321 ttccgcgccc cggtccgcgt ttcctgcggc cccgcccctg acgtccgcct gaggatgtat
     1381 cggtcgcgcc ggtgccggac agtcctcctg tgggccagtt tgttacaagt actggctgaa
     1441 cggctgccgg atttaccccg gtaagaaagt ttataaaacg ttgccctcgt ccctgagatg
     1501 tgcctggtgg tgttccggcc ggaggagagc gccggaaagg tgaggtcagg aaggccgccg
     1561 ctgataatcc gtatcccagc aatgcagcac ccggacgtat aatccccgtt ccgacagccc
     1621 gcgccatttt cagggcacgg gtggcgagat acatgtacag caggtatttc gcggcggtct
     1681 gtgccccctg accaatgcga tccagcgcat caccatagcc cgcatcccgc agagcctgaa
     1741 gcgtgtcgcg tacctt
//
LOCUS       DAZFTN010000318         1751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFTN010000318 DAZFTN010000000
VERSION     DAZFTN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..301)
                     /locus_tag="ACHKV2_005395"
     CDS             complement(<1..301)
                     /locus_tag="ACHKV2_005395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6943064.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFS"
     gene            complement(439..635)
                     /locus_tag="ACHKV2_005396"
                     /pseudo
     CDS             complement(439..635)
                     /locus_tag="ACHKV2_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(922..1740)
                     /locus_tag="ACHKV2_005397"
     CDS             complement(922..1740)
                     /locus_tag="ACHKV2_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO6943065.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          625 a          402 c          310 g          414 t
ORIGIN      
        1 cggaaaatct ctggctggta attaaagcct cagccaacgc agggttgttg ggttccagtg
       61 acagataaag cggaacgtca gtcagatgag ttcttgtcag cttatgctga atttccagat
      121 attgacttac tatggaatgg tttatatcga aaattttaac tttgccataa tgcataagga
      181 aaagcgccct gatgcatttg gtggccagaa cgactgagcc gggcttaagt gacaacgtat
      241 ccttttcaag aaaaatatta attggggagc aaaccatgat aactgaacag acaacaacca
      301 ttataatttt actttcatta gcaattggtt agttcaatta tagccccaaa aggtaaatta
      361 tcatcaacac ataagcaaag gactgacagg tgccgctaac acccaccagc cgcccattta
      421 ccacaaataa aaaagccttc aggactgaag gcgtctgtaa caaccgaact gatagtctgc
      481 cagacccgca taaccagctg ggtcagtatt aactggcagc gctcgcgtga aaggtacgta
      541 ttctgcgcaa tctccccgac cgtcgccggt tcggtgacgc ttaattcatc aaacacaact
      601 ctggcggttt ctgtcatatc ctgctgtttc agcatgtctt tttacccttt ccggttaacg
      661 tgacacacca ataactcttg tcgaaaaagc cagcaagctg aaagacctgt attaataact
      721 accagcacat ttaacgcact gcgctacttt gcgggcacaa aaaacccgct cagaggcggg
      781 gtcaagctat gcggcgaaat aaccactctt aacagcatac ctgatttttt acgtacgtaa
      841 atgctttgcc gtgcatattt ttcatgcaaa tgtcacgtcc tgctattttt cagtcttata
      901 aatttaaaac catagaaaaa atcaattatg ttttaaaaat ggataggtaa agaataacaa
      961 gtgacacaga ttcaaaccaa aatggaaaag ggtggcaacc cacaaacgcc cactccacat
     1021 ccatgacaat ccatacacaa caccagataa cgtggcaaat aatacaagta aagcaccacc
     1081 tgaatagtga taaaaaccaa acaacaaagc cgccacaatt aatgcaacca atggagacgt
     1141 tacttctgat agccgtgatt gaatataccc tctaaataat gattcctctg ccagagacac
     1201 aaaaaacaaa ttagccaata taaactctgg caaccactca ggaaaatgaa tctctggctt
     1261 taatccacca aaaaaaacag ccaaaaacag gataagagga acagagagcg acagagcccc
     1321 ccacttccac aaagacactt ctgattttac ttctttttta aacaaagaag atgtacacag
     1381 gactaacaaa aatggcacca gcgctttatc aaaattaaaa tacattgtat agggagtact
     1441 ttgagggcca acagtaacag aatttagcac tacaggattg tgaaaccctg gccatagatg
     1501 gaaaaataat gctatggctg ataaaactat gccaacttca tatatagatt tagcccaggc
     1561 gttatatttc cagttgaact tcaaaataat aaaaaaaacg attgtagcaa caaaaaataa
     1621 caccgaccaa tcaataatat cattaagcac agccaggaca acagacaccg tcaacaatga
     1681 aaaagccact accttatgcc aactgagagt tgacagtgac agtactaaca caatccacat
     1741 aagcactcct t
//
LOCUS       DAZFTN010000319         1743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFTN010000319 DAZFTN010000000
VERSION     DAZFTN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1660
                     /gene="tir"
                     /locus_tag="ACHKV2_005398"
     CDS             <1..1660
                     /gene="tir"
                     /locus_tag="ACHKV2_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO6943066.1"
                     /translation="GHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTP
                     VRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVF
                     RVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGGAGHAMV
                     TVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSS
                     LRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATETATRDQ
                     LTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQA
                     QKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQTTTTTT
                     TTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQN
                     PYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGNPSAG
                     IQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          483 a          381 c          466 g          413 t
ORIGIN      
        1 tggtcataat cccaatgtga ataattcaat tcctcctgca cctccattac cttcacaaac
       61 cgacggtgca ggggggcgtg gtcagctcat taactctacg gggccgttgg gatctcgtgc
      121 gctatttacg cctgtaagga attctatggc tgattctggc gacaatcgtg ccagtgatgt
      181 tcctggactt cctgtaaatc cgatgcgcct ggcggcgtct gagataacac tgaatgatgg
      241 atttgaagtt cttcatgatc atggtccgct cgatactctt aacaggcaga ttggctcttc
      301 ggtatttcga gttgaaactc aggaagatgg taaacatatt gctgtcggtc agaggaatgg
      361 tgttgagacc tctgttgttt taagtgatca agagtacgct cgcttgcagt ccattgatcc
      421 tgaaggtaaa gacaaatttg tatttactgg aggccgtggt ggtgctgggc atgctatggt
      481 caccgttgct tcagatatca cggaagcccg ccaaaggata ctggagctgt tagagcccaa
      541 agggaccggg gagtccaaag gtgctgggga gtcaaaaggc gttggggagt tgagggagtc
      601 aaatagcggt gcggaaaaca ccacagaaac tcagacctca acctcaactt ccagccttcg
      661 ttcagatcct aaactttggt tggcgttggg gactgttgct acaggtctga tagggttggc
      721 ggcgacgggt attgtacagg cgcttgcatt gacgccggag ccggatagcc caaccacgac
      781 cgaccctgat gcagctgcaa gtgcaactga aactgcgaca agagatcagt taacgaaaga
      841 agcgttccag aacccagata atcaaaaagt taatatcgat gagctcggaa atgcgattcc
      901 gtcaggggta ttgaaagatg atgttgttgc gaatatagaa gagcaggcta aagcagcagg
      961 cgaagaggcc aaacagcaag ccattgaaaa taatgctcag gcgcaaaaaa aatatgatga
     1021 acaacaagct aaacgccagg aggagctgaa agtttcatcg ggggctggct acggtcttag
     1081 tggcgcattg attcttggtg ggggaattgg tgttgccgtc accgctgcgc ttcatcgaaa
     1141 aaatcagccg gtagaacaaa caacaacaac tactactaca actacaacta caagcgcacg
     1201 tacggtagag aataagcctg caaataatac acctgcacag ggcaatgtag atacccctgg
     1261 gtcagaagat accatggaga gcagacgtag ctcgatggct agcacctcgt cgactttctt
     1321 tgacacttcc agcataggga ccgtgcagaa tccgtatgct gatgttaaaa catcgctgca
     1381 tgattcgcag gtgccgactt ctaattctaa tacgtctgtt cagaatatgg ggaatacaga
     1441 ttctgttgta tatagcacca ttcaacatcc tccccgggat actactgata acggcgcacg
     1501 gttattagga aatccaagtg cggggattca aagcacttat gcgcgtctgg cgctaagtgg
     1561 tggattacgc catgacatgg gaggattaac gggggggagt aatagcgctg tgaatacttc
     1621 gaataaccca ccagcgccgg gatcccatcg tttcgtctaa atatatccat aatcatttta
     1681 tttagaggga gggagggggg aagtctaact aacgtcaatt tttcagggaa taacattgct
     1741 gca
//
LOCUS       DAZFTN010000320         1696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFTN010000320 DAZFTN010000000
VERSION     DAZFTN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1401
                     /locus_tag="ACHKV2_005399"
     CDS             <1..1401
                     /locus_tag="ACHKV2_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943067.1"
                     /translation="LIDNASLTAVERTLGDILVKNPDTINGDLVAFENLIQAILFYDT
                     LICVDNYKKEYRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGEFVDD
                     DFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELSDASD
                     TKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAYKSIY
                     YSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSEIVDH
                     GRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTYDESG
                     ISAGNKKVTKLLSDLDKISGDIKRSYGVPSNQGIQGSFLIKCINSLTGLVGIPGLPDK
                     EFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDDSYYTEPKT
                     EDPRFRYVSSNWKQPM"
BASE COUNT          565 a          264 c          336 g          531 t
ORIGIN      
        1 ttgattgata atgcttcgtt aacagcagtc gaaagaacac tgggtgatat tcttgttaag
       61 aatccagata ctattaacgg agatttagtc gcttttgaaa atcttattca agcgatactt
      121 ttttacgata ccctgatctg tgtagataat tataaaaaag aatatagaga taaacgcatt
      181 gctaaatttg actttattaa atttgtatct gaaagtgatt ttcaactcag tgaacttgat
      241 cagttagcac aagttgaatc tcggcaaatt acttccgaaa ttagaggtgg ggagtttgtt
      301 gacgatgatt ttaggcaact tgtagagatg cttaagctga atatgatttg cacatgggat
      361 cttcggtcta gcgtgtatta cttaacaatg aaaatgcttg gtcagcccgg tactcctgaa
      421 tatgcgaaat atagtgaact aagtgcatcg atttttaatg aattatcaga tgcttctgat
      481 acgaaagggt actggtcaac cgatgtgaag ctagtgagtt catctggaca tgaatatact
      541 gagcgtgaat ttgaactaga aagggaaaaa tctactcgcg gtaaaggagg tatgactcgt
      601 tcacttgaaa tgtttatcgc atcgctaaat tggttggcat ataaatctat ttattacagc
      661 gtcattgcta agcattttaa agcagactca tttattcatc caataaggca tgcatatcaa
      721 ttgcattgga tgaaaaaaac aggtgctttt ggtcatgatt atactgcaag gttggtgcaa
      781 tcgttgtctg ataaaatcag tactgcaaga tctgaaattg tagatcatgg acgcacatct
      841 actatttcat tagatttacc aatattttct gcatggctag caaacgaaac aggaaatgtc
      901 tctcaagtta tcaattcagc tttagagctt agaacacatg atcattttcg tacgtgtcga
      961 gatgtaatcc gagagattca tgtgacctac gatgaatcgg ggataagtgc aggaaataaa
     1021 aaagtaacaa aattactaag tgatttagat aaaatatctg gagatattaa acgaagctac
     1081 ggtgttccat caaatcaagg tattcaaggc agttttttga taaaatgtat aaattcattg
     1141 acaggactcg tgggtattcc aggtttgcct gataaagagt ttgcactgag cactcctgaa
     1201 tttatgaagt ctaaacaaca taaagcattt tctaccgttt tcaaggatgt tactaacgaa
     1261 ctaacaagca ttgaacgttt aggtgggctt cgggataaat tagcgtcgaa ctttactatt
     1321 gatgattctt attataccga gccaaaaaca gaggatccga gattccgtta cgtaagtagt
     1381 aattggaagc agccaatgta gatatagtta aatgtataat tataagtaat aacaatttac
     1441 agacattatt gaattaaaaa tattgcgtta gggtataata tctaacgcaa ttagattgaa
     1501 aagttaatta aatagttagt cagagctaac ttcttgtatt gttagttaca ctgcctaacc
     1561 tacagacaaa aagcccctca gaattgtgct tacgcgctca aatttcactg ctggggattt
     1621 ttacgtacat tcaacccaaa aagtgaccaa aaaagttctt acagagttga cgttacgttg
     1681 acgttgtttt ctgaaa
//
LOCUS       DAZFTN010000321         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFTN010000321 DAZFTN010000000
VERSION     DAZFTN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..858
                     /locus_tag="ACHKV2_005400"
     CDS             <1..858
                     /locus_tag="ACHKV2_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6943068.1"
                     /translation="GIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRSAFTA
                     LQQGKRLNVRQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYSPAEFR
                     KKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGRKVLI
                     SYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAEEKIR
                     DAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGLQLLEA
                     KLKENDPLA"
     gene            930..>1692
                     /locus_tag="ACHKV2_005401"
     CDS             930..>1692
                     /locus_tag="ACHKV2_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6943069.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRTFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAEL"
BASE COUNT          433 a          407 c          500 g          352 t
ORIGIN      
        1 ggcattatcg aacggcttaa ccgcacatta ccgatgcgca tagcccgtaa ataccgcacc
       61 tatttcggga aaggtgcaga tcgcgagacg ttacgcaaaa ccaaccgcga tttacgctcg
      121 gcatttactg ccctgcaaca gggcaaacgg ctgaacgtcc ggcagcagtc agcgatgcgt
      181 gatttaccgt cctggtctga actgattgat gccattcgtg acggtgttga gtggtacaac
      241 aaccggccgc acgatgaatt accggtgaag ccgaacggca agcattacag cccggcggag
      301 ttcagaaaaa aacgcctggc ggaagaggac acggaaattg aatggctgtc cgatgttgag
      361 ctgcgggata tgttccggcc gatggtggaa cgccctgtaa gacgctgtga aatacgctgg
      421 ctgaataata tttactacgc gcccgagctg cgtgacgagc atggtcgtaa ggtgcttatc
      481 agctatgaca ttcatgatgc cgaacgaatt accgtacgtc gcctggatgg cagcgtgatt
      541 tgcgaggcgg tatgggacgg taataaacgc gaagccttcc cggttagcgc ggaatactac
      601 aaacagcagc aacgccttaa aggcatgcgt aaacgcgcag aggaaaaaat ccgtgatgcc
      661 gaggatgagg ttgtcaacgt gctggagcac aagccgcagg agccctggct ggaaaacata
      721 taccgccctg tgggtaatac ggtgaccgtt cagcaaccgg tcgctgacga tgaacctgat
      781 gaagaatacg agcgtaattt ccagcgggga ttgcagttgc tggaagcgaa attaaaagaa
      841 aatgacccgc tggcctgaaa taaaaaaata acccgagcgg cgactcaggt tatttgatta
      901 aacaaggtat caaatgagag gttaataata tgactgatat taacgatgta atcaagacca
      961 ttgatgaact tattgatggc ggcgtactga cgcagtatgc catcgccaga gaggcgggaa
     1021 tttccgacgg cacattatcg gctttccgta agggaaaata taaaggcgat aacgctgctg
     1081 tggctgcttc cctgcgttcc tggtatgaga actggaataa acaaagcgca ctgccggaac
     1141 cgccgcagtt tgtggaaacg cagacagtcc aggagctgcg cgcactgttt caggcggttc
     1201 gtctgatggg ctgtattaac gttattgtgg gcgtaccggg tgtgggtaaa acggccactg
     1261 cccgtaatta ctgccaggag caaccaaaca cctggatgat caccctgtca cccgcgcact
     1321 ccagcgtcac ggagtgtctg ctggagctgg ccgatgcgct ggggattgat tacacccgcg
     1381 cgaacaaagg ggcattatcc cgcgccatcc gccgtcgcct gatgggaacg cgtggactgg
     1441 tgattgtgga tgaggcggat catcttggta ttgacggtct ggagcaactc cgggcaattc
     1501 aggacgccac ggggatcggg atggtcctta ttggtaaccc gcgcggattg tttaaaggtg
     1561 gacgccgcac ctttgatgat ttatcgcgcc tgttcagccg tcttgcccgt acaaaacaac
     1621 ttcgcaaggc caaaaaggcg gatgtgctgg ccattgccag ggcgtggggg atcagtggtg
     1681 aggccgagct gg
//
LOCUS       DAZFTN010000322         1690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFTN010000322 DAZFTN010000000
VERSION     DAZFTN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1690
                     /locus_tag="ACHKV2_005402"
     CDS             <1..>1690
                     /locus_tag="ACHKV2_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6943070.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DAZFTN010000323         1689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFTN010000323 DAZFTN010000000
VERSION     DAZFTN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            297..458
                     /locus_tag="ACHKV2_005403"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHKV2_005403"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHKV2_005404"
     CDS             442..507
                     /locus_tag="ACHKV2_005404"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO6943071.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHKV2_005405"
     CDS             623..880
                     /locus_tag="ACHKV2_005405"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6943072.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHKV2_005406"
     CDS             complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHKV2_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO6943073.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1562..>1689)
                     /locus_tag="ACHKV2_005407"
     CDS             complement(1562..>1689)
                     /locus_tag="ACHKV2_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO6943074.1"
                     /translation="EFNPIDISSYNVSDISWSEHQVMQSYHGGKLDLISVVLSKI"
BASE COUNT          524 a          378 c          317 g          470 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga ataaagattt gctgaagaac cacttgttgg taacatatca
     1501 aaaccccctt aacaaataaa tccataaata ttattgcaac taatatatct ggataaaaat
     1561 attatatctt acttaatact acactaataa gatccagctt tcctccgtga taagattgca
     1621 tgacttgatg ttcactccat gaaatatcag aaacattata agatgatata tctattggat
     1681 taaattcat
//
LOCUS       DAZFTN010000324         1686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFTN010000324 DAZFTN010000000
VERSION     DAZFTN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..280
                     /locus_tag="ACHKV2_005408"
     CDS             <1..280
                     /locus_tag="ACHKV2_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO6943075.1"
                     /translation="YNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQF
                     FSEHPEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(341..1462)
                     /locus_tag="ACHKV2_005409"
     CDS             complement(341..1462)
                     /locus_tag="ACHKV2_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO6943076.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDEQLSSEDKYIEGEDTKSENNDNNLKN"
     gene            complement(1471..>1686)
                     /locus_tag="ACHKV2_005410"
     CDS             complement(1471..>1686)
                     /locus_tag="ACHKV2_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6943077.1"
                     /translation="ASPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLILI
                     IYFGPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          554 a          316 c          271 g          545 t
ORIGIN      
        1 atataatagt aaaatcataa taaccatcgc gctgatatcg ttgctctctg catgcaccag
       61 agaaaattca ttaaaggaga atcaaaataa aagaagtggc gaatcccaag cgatagtaga
      121 tgcaagacag ttctttagcg aacacccgga atatatatca tcagcagaac tggaggaaaa
      181 gctatatcgc gaatttgcac aggttactac agttccagct tataaagaga tgagtatgta
      241 tcaattgctt gtcatttcgc aagatcgttt aactcattaa ataaaaatct acacgttttt
      301 tgcgtgtaga ttcttataaa tttttgacta cgatacatgt ttaatttttt aaattattgt
      361 cattattctc gctttttgtg tcttcaccct caatatattt atcttctgaa gagagctgtt
      421 catctggaac aggttgtcca gcattctcca catcttcaag ccacagcaga agacgtaata
      481 tttcatctat attttcgaag ctaacataat cgtagcgacg atgggtagca taaatttttc
      541 gtgctaattt tttatctgta ataattggta tcccgatttc ctttgcatat ttccttacag
      601 ctaatgctac ctcattagtt tctcttacag aaatcaatgg aataggtgac agatgtggct
      661 taaagtatat ccctattgca atgtgagtag ggttggcaat catcaaacgg ctattactga
      721 catcagattt caattgctca gaaagaattt cctgatggcg ctctctgcgt ttagacttaa
      781 tttcaggatt tccttcttgt tccttgtatt ctcttttaac ttcttgttta tccattttca
      841 tatctttcat aaataaaaaa tattcagcga taaaatcaaa aattaagaca attatcatac
      901 tgccgagaca atacagtatg agaaggaata gcatctctcc ccaatctgat aataaagata
      961 tgatatctcc atcaagagtt ttaaaaagca gtgatttatg attactccaa aatactttga
     1021 tctccaatgc aaaaaaaata atataaagaa ttgctttaac aaattctttt acggttttta
     1081 agccaaatat acgctttaaa ccatttactg gattcaatga atcaaacttt aattttacag
     1141 cttcagttgc taattgcatt ttactttgaa accaggagac aagtattgtt gtgaacatgc
     1201 aaaccagcaa aaaaggaatg atcgttttca accaaccaat ccccatagct ttaaaatagt
     1261 cccaaatatc cggtttagcg ttatgatcaa ggatatattc aagaatcgac ataatgtgat
     1321 gcacatcaaa aacatatccg agatataaag tacccacaag cattattaca gaaacggtta
     1381 agtctctact ttttagaatt tgcccttttt tagaagcatc ctgcagtttc ttttgtgttg
     1441 gcttttcagt tttatttgcc atgaaacact ctatttctca atgtatagtt gcaattgttc
     1501 tgggaaaaag gataacggca tcactcgttc agccagtatt gggccaaaat aaataattaa
     1561 tattaaaaat gccagcgcac ttttgacagt cagtgaaatg gcaaaagcat ttagttgtga
     1621 agcatagcgt gccagcaatc ccaggacagc ctcaccaccc agcataacgg caattacggg
     1681 gcttgc
//
LOCUS       DAZFTN010000325         1674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFTN010000325 DAZFTN010000000
VERSION     DAZFTN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..246
                     /locus_tag="ACHKV2_005411"
     CDS             <1..246
                     /locus_tag="ACHKV2_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943078.1"
                     /translation="QSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKTTPP
                     VGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            248..529
                     /locus_tag="ACHKV2_005412"
     CDS             248..529
                     /locus_tag="ACHKV2_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO6943079.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIDVVA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMVQFCREVRRRVSYPNRTRARG"
     gene            526..798
                     /locus_tag="ACHKV2_005413"
     CDS             526..798
                     /locus_tag="ACHKV2_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943080.1"
                     /translation="MSKVVRIIFEYKEDVIRKNADGTVHMGVSLDIRSTGIKQKNDSP
                     AMIFGVVMLAESRNFAELVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            803..1096
                     /locus_tag="ACHKV2_005414"
     CDS             803..1096
                     /locus_tag="ACHKV2_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943081.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            1108..1638
                     /locus_tag="ACHKV2_005415"
     CDS             1108..1638
                     /locus_tag="ACHKV2_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6943082.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRRFGDMQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
BASE COUNT          481 a          365 c          472 g          356 t
ORIGIN      
        1 caatcctgcg gtaacaaaat gaatttattc gactccctga acagcgcgcg ccgtctgacc
       61 gaacttgccg gtgcggtact ggaacgcagt aagcgctatc cgcaacgttt tgcactgaaa
      121 accacgccgc cggtaggcaa cgtgcaggga accggtgaaa ttgaaatcac catacagacc
      181 aacggcctgc gccgccgtgt gaaggccacc cgcatcagcg gctgcacggt ttactgggag
      241 gtgtgaggtg aaaaaaaatc tcattgcatg ggcgtggtcg agtggtctta ttgagtttgg
      301 ttacgtcctg ccggaaggtg cattgccgat agttgccgga aagcccgcca cggtacggca
      361 tgtgattgat gttgtggcgc gtcatggacg cgatgaacag gaacagctac tggttcctgg
      421 aataccggaa gcggtgacgg aagaagaagc ctttaatgcc atggttcagt tctgccgtga
      481 ggtcagacgc cgggtcagtt atccaaacag aacgagggcc agaggatgag taaagtcgtg
      541 cgcattattt tcgaatacaa ggaggacgtt atccgtaaaa acgctgatgg aacagtgcac
      601 atgggggtaa gtctggatat acgttcaacc ggaataaagc agaaaaatga tagccccgcc
      661 atgatttttg gggtggttat gctggcagaa agcagaaact tcgcagagct tgtggcaatg
      721 aaagccagtg cattcatgaa agacaggggc atggcttccg gggttattaa cggtaatgaa
      781 tttaatcagc aggggtaatt ccatgagcaa agtacgcgtt atttttgaat ttgagcatgt
      841 ttcgcatgac gaaaaaccgg caggcaatga cagtgttgaa gtgcatgaaa agattggtgt
      901 ggatgtaaaa acagaacgtg atacggataa caggccgacg tcactctgtg acgtttatgc
      961 aagtattctc cagtatcaca gtcctgaaat tattcagttt ctctcagcgg aatttcaggc
     1021 atctgtacag gcttttgggg cggatgccat cattaaacgc caccgcgtgc ataaagcatc
     1081 aggcacactg caataaggaa aaacaaaatg gctaaacgcg ttacaaaatt aaaggccgca
     1141 gccgaggcag caccgcagac ccgtgaagag gtcagccgcg atatccgacg ctttggggat
     1201 atgcagcgtg aggcactacg cctggaaacg gcgatgaatg acgaagtggc agaaatcacc
     1261 gcccgttata cgccgcagat tgaaaacctt aaaaaagaaa tcaaagtgct ttttaagggg
     1321 attcatgact ggtgtaaaac caaccgcaat gagctgacga acggcggcga aaccaaaaca
     1381 gccaatctga ccaccggaac ggtgtcatgg cggctgggaa cgccatcatg cagcgtcagc
     1441 cgtgatgtgg aaggcgtgat tgaaatgctg cgccgtatgg gccttgagcg cttcatccgc
     1501 acgaaagagg aagtgaataa gcaagctgtc ctggcggagc cagatgcggt gaagggcatt
     1561 gccggtataa aggtgaataa aggcgctgaa agtttttatg tcgagccttt tgaacaggac
     1621 gccggactga ataaataaca ccgcattaaa tctttaaata tcacatcgtg ttaa
//
LOCUS       DAZFTN010000326         1657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFTN010000326 DAZFTN010000000
VERSION     DAZFTN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1657
                     /locus_tag="ACHKV2_005416"
     CDS             <1..>1657
                     /locus_tag="ACHKV2_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO6943083.1"
                     /translation="VTDLQGRLYRVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSV
                     IGLVQYARMIKMNDNISAIDHAGAVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASL
                     EGFISSGLEVCASRMGGTAGRYLSNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQI
                     EYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKL
                     WLEAEKYLDNGSAKVLSINKATGIIDLSNNQVLGNIYLDMRENPPILHGEKSYNSGKN
                     IGSHPDMTDREIMESRAYNFACTKLSDAGEPDIFGWGDKEICNSMELSESQLANGYSN
                     RQWPSQIPVIPEGIYNTVYLGYGETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLT
                     VCNQHSHVIGGKEPLTIIIPAIEHGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGI
                     GDYNIECTPGVRNIISFEQLSRDFNLDLDLSDGRKQNFNFHHPSGFYSGKVMSITQKG
                     INAVVGTKLGYDKIRGNNLDNTFSLGSGGGVIYSGGGSNTYFVP"
BASE COUNT          550 a          245 c          344 g          518 t
ORIGIN      
        1 agtaacagat ctgcaaggaa gactttatcg tgttgatata gatacaagag ttattggctt
       61 aacttttaaa gaggggataa atagtttatc cgaagcactc gaacacatga atatcgatgc
      121 gattatgtct gttattggct tggttcaata tgcacgaatg ataaaaatga atgacaatat
      181 tagtgcaata gatcatgcag gagcagtatc agatataaaa aatattgtag acaaattttt
      241 aggcggaatt ttgactctta ctaataatcg cgtatataat cctggcggtg tatctggtgc
      301 ctcgcttgaa ggatttatat cttctgggtt ggaggtgtgt gcttcaagaa tgggtggcac
      361 cgcagggcgt tatcttagca atgtggcaaa agtaattaaa ttacctttgc tagatatagg
      421 cataaatatt tggtcattat atgattcttc attaaatcat gctaaagcca ccacacaaat
      481 tgaatatata tcaacagcaa tagatgtatc tttttcatct ataaatacag ctttgtcaat
      541 aggcgcaatc gcatatcctc ctttggcaat agctatagtt ccgataacta ttttttctca
      601 tgaagtaaaa aactatgctg tttatgtgaa tcaaattaat gagcgccata agttatggct
      661 cgaagctgaa aagtatctgg acaatggaag tgcaaaggtt ctaagcatta acaaagccac
      721 aggcataatc gatttatcaa ataatcaggt gttaggtaat atttatttag atatgagaga
      781 aaatcctcca atattacacg gtgagaaatc atataatagc ggaaagaata ttggtagtca
      841 tcctgatatg actgacagag agattatgga gtctagagca tacaattttg catgcacaaa
      901 actctcagat gcaggtgaac ccgatatttt cggttgggga gataaggaaa tatgtaattc
      961 aatggaatta tcagaatcac aattagctaa tgggtattct aaccgccaat ggccttctca
     1021 gattccagtt attccagaag gaatttataa tacagtatat cttggatatg gagaaacatt
     1081 gcgagctaat actgaagtta cattgagtag cactggttac ttttacgaga ttgcaagggc
     1141 atatactgat gatgaactat ctgagccatt attaacagta tgtaaccaac atagtcatgt
     1201 tattggtgga aaagaaccat taacaattat tattcccgct attgaacatg gtatgcttgg
     1261 ctctaatcta catatgattg aacgttttaa aaactataat ttctcgatat caggagggaa
     1321 gggagggata aaattaatgg ttggtggtat tggtgattac aacatagaat gtacccccgg
     1381 tgttagaaat attatatcgt ttgaacaatt atcacgtgac ttcaatctgg atttggattt
     1441 atcagatggg aggaaacaaa attttaattt tcatcatccc tcaggttttt attcaggaaa
     1501 agttatgagc ataacgcaaa agggtataaa tgcggttgtt ggtactaaac tgggatatga
     1561 taagattcgt ggtaataatc ttgataatac cttttctctt gggagtggag gaggtgttat
     1621 atattctgga ggagggagca atacctattt cgttccc
//
LOCUS       DAZFTN010000327         1646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFTN010000327 DAZFTN010000000
VERSION     DAZFTN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..1124
                     /gene="ompC"
                     /locus_tag="ACHKV2_005417"
     CDS             24..1124
                     /gene="ompC"
                     /locus_tag="ACHKV2_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6943084.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1287..>1646
                     /locus_tag="ACHKV2_005418"
     CDS             1287..>1646
                     /locus_tag="ACHKV2_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO6943085.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNASLVNINLSFNKINTI"
BASE COUNT          490 a          336 c          343 g          477 t
ORIGIN      
        1 gataattaaa aggattctgt taaatgaaat taaaaatagt tgcggtggtt gtaactggtt
       61 tgttagctgc gaacgtagca cacgctgccg aagtctataa caaggatggt aataaactcg
      121 acctttatgg caaggttacc gctctacgtt attttactga tgataagcgt gacgatggtg
      181 ataaaactta tgcccgtctc ggctttaaag gagaaacgca aatcaatgat caaatgattg
      241 gttttggtca ctgggaatat gattttaaag gctataacga tgaagccaac ggctcgcgcg
      301 acaacaagac ccgtctggcc tatgctggtt taaaaattag tgaatttggc tctctggact
      361 atggccgtaa ctacggtgtc ggctatgaca ttggttcatg gactgatatg ttgccagaat
      421 ttggtggcga tacctggagt cagaaagatg tcttcatgac ataccgtacc accggtgtag
      481 caacctatcg caactacgat ttctttggct taattgaagg gctgaacttt gccgcgcaat
      541 atcaaggcaa aaatgaacgt actgacaaca gtcatcttta tggtgctgac tacacgcgtg
      601 ccaacggtga cggtttcggt atctcctcaa cttatgttta tgatggcttt ggtatcggag
      661 cggtgtatac caaatccgat cggacaaatg cgcaggaaag agccgctgct aatcctctca
      721 atgcctccgg taagaatgca gaactgtggg ctacaggtat aaaatatgat gccaacaaca
      781 tctactttgc agctaattac gctgaaacat taaacatgac cacctatggc gatggttata
      841 tttctaacaa agcacaaagt tttgaagtgg tggcgcaata tcaattcgac ttcggcttgc
      901 gcccctcact cgcttacctg aaatcgaaag gcatagatct gggccgctac ggcgatcagg
      961 acatgattga gtatatcgac gttggtgcga cgtatttctt caacaaaaat atgtcgacct
     1021 atgttgatta taaaatcaac ctgattgatg aaagcgactt tacccgtgcc gtagatattc
     1081 gcaccgataa catcgtcgct acgggcatta cctatcagtt ctaacatcct ctcctgacca
     1141 gcccttctct gggctggttt ttgatttaag tgctgctgta tgactcctta agtaaaaatg
     1201 agcaattttt gatatttctg gtcattcacc tcaattaata ttattccata cccccgggga
     1261 catcttacta taatatttat tgatgagtga ttatgaccaa tattaataca gcttgtgtaa
     1321 aaaataatgc cagttatcaa tttaataacg cattacctaa caaagaaacc atctccagca
     1381 atttttgtga acgactagaa caatggggta ataagtcgct taataatggt gaagaaagag
     1441 caattgccgt ggagcgaatt aaagaagctt acaattcgaa catggcatct cttgatttgt
     1501 catatcttga tttgagtgag ttaccgccta tcccctcgac agttaatacg ttaaatctgg
     1561 aaaacaattg tctaacttgt cttgacttta ctgacaatgc cagtctcgtt aatatcaacc
     1621 tcagctttaa taaaattaac acgata
//
LOCUS       DAZFTN010000328         1633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFTN010000328 DAZFTN010000000
VERSION     DAZFTN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            51..>1633
                     /locus_tag="ACHKV2_005419"
     CDS             51..>1633
                     /locus_tag="ACHKV2_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO6943086.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGN"
BASE COUNT          471 a          373 c          375 g          414 t
ORIGIN      
        1 ataattatca ttacagcaat catttgtact ttgtattaat gagggatgaa atgttatata
       61 atataccttg tcgaatttat atcctttcca ctctgtcatt atgcatttct gggatagttt
      121 ctactgcaac cgcaacttct tcagaaacaa aaatcagcaa cgaagagacg ctcgtcgtga
      181 ccacgaatcg ttcggcaagc aacctttggg aaagcccggc gactatacag gttattgacc
      241 aacaaacatt gcagaactcc accaatgcct ccatagccga taatttgcag gacatccccg
      301 gagtagagat aacagacaac tccttggcag gccgtaaaca aatccgcatt cgtggcgaag
      361 catcctcccg tgttttaatt ctcattgatg gtcaggaggt aacttatcag cgcgccggag
      421 ataattatgg tgtgggactg ttgatagatg agtctgcgct ggagcgtgtt gaggtagtga
      481 aaggtccata ttccgtactg tacggttcac aggcaattgg cggtattgtt aacttcatca
      541 ccaaaaaggg aggtgacaaa cttgcatctg gagttgtgaa agctgtttat aattccgcaa
      601 cagcaggctg ggaagaatca atcgcggtcc aggggagcat cggtggattt gattatcgca
      661 tcaacggtag ttattctgat cagggcaatc gtgatacgcc ggatggacgt ctgccgaata
      721 ccaactatcg taacaatagt cagggtgtat ggttgggtta taactccgga aaccatcgtt
      781 ttggcctctc gcttgatcgc tacagactcg cgacacaaac ttactatgag gatccagacg
      841 gaagttatga ggcatttagt gtcaaaatac ctaaacttga acgagagaaa gttggggtat
      901 tctatgacac agacgtggac ggtgactatc taaaaaaaat tcatttcgac gcgtatgagc
      961 agaccatcca gcgccaattt gccaacgaag taaaaacgac acagcctgtt cccagtccga
     1021 tgattcaggc tctgaccgtt cataacaaga ctgacaccca tgataagcaa tacactcagg
     1081 cggtcacatt gcagagtcac ttttcgctgc ctgctaataa tgaacttgtt accggtgcac
     1141 agtacaaaca agatagggtc agccaaaggt ccggtggcat gacctcaagc aaatctctga
     1201 ccggcttcat taataaggaa acacgaactc gctcctatta tgagtcagag caaagtacag
     1261 tctcactatt cgcacaaaat gactggcaat tcgccgatca ctggacatgg acaatgggag
     1321 ttcgccaata ctggctttct tcaaagttga cgcgtggtga cggagtatca tataccgcag
     1381 gcattataag cgatacctct cttgccagag agtctgcgag tgatcacgaa atggtaacat
     1441 ctacaagcct gcgctattca ggtttcgata acctggagtt acgcgctgcg ttcgcgcaag
     1501 gctacgtatt tcccacactc tcccagcttt ttatgcagac atctgcgggc ggcagtgtca
     1561 catacggaaa tcctgatctt aaggctgaac actccaataa ctttgaatta ggtgcacgat
     1621 ataatggtaa tca
//
LOCUS       DAZFTN010000329         1589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFTN010000329 DAZFTN010000000
VERSION     DAZFTN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(92..268)
                     /locus_tag="ACHKV2_005420"
     CDS             complement(92..268)
                     /locus_tag="ACHKV2_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943087.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            571..1197
                     /locus_tag="ACHKV2_005421"
     CDS             571..1197
                     /locus_tag="ACHKV2_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6943088.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
BASE COUNT          493 a          357 c          354 g          385 t
ORIGIN      
        1 atctactgat tttgatggtg aaatttatta gaaggtatgc gtagtgctac gggagaaatg
       61 agaaacgcgc ccagcaatgg cgcatccttt cttaagcaag ttggctagta tctatagcat
      121 ccatccaatc aggagcaatt cccaaacgtt ccgcgaactc ttcattggtc acgtcttcca
      181 ttgagagact agccagacaa tcgttaattt cagaatagac gggggaaagt aataggtatt
      241 cccgcaactc tttcaggtct ttttccatct taacactcct tgttaataga ccgtcgaaca
      301 atggtacaga tttgtaccat tccaagtact ggcaagcaac cccaccagca caacaaatta
      361 tctcatacgg taaaaacggt gtcgggctgt ttctttcggc tagctcaata tatcacctta
      421 aacaccagcc gttatcattg tctcaattgg gtataccgta atgacaaggt gatacaaagt
      481 ctcaataggg ttgtatagga acattttgac tacacaaact gcattatgta tcatttgcac
      541 ttatattcac aacccttttg agacaatgca atgaacgtta gaatttactg ccgagcatca
      601 actgaggggc aacacgctga tcgagcactt accagcctac gtgaattttc aaaatcaaaa
      661 ggctggcaga ttgcaggaga gtatatagaa aatgctagcg gggcgaagct tgagcgcgta
      721 gagcttatgc ggctattgtc tgaagcccag tccggcgatc tgttgctagt ggaagctata
      781 gatcgtctga gtcgcctaga acatagtgca tgggtcgaac tgaaagatac gctcaaccga
      841 aaagggttaa tcatcgtatc aatggattta ccaacgagct ggcagatggt cgagatggca
      901 ggcaatgatc taaccagcgg catcctgaga gccgtaaatg ccatgttgat tgatatcctt
      961 gccacaatgg cacggcagga ttacgaaaca cgccgtaaac gccaacagca aggcattgaa
     1021 cgagctaaaa gcgaaggcat atacattggg agggcgaaaa atcaggaagc ccgagagatc
     1081 gttagagaga tgttggagca aggagtaaaa cctgaactca tcatgaaggc agcaggaatc
     1141 agccgagcga cttactaccg gataaaaaac gagctattga tcgtaaaatc agaataaaat
     1201 ttgaaaggtc gtcaggcttg tttcatcagg taaaaaccag catttctgat cacaacgctt
     1261 gggggcacct attggggcac tcaccaaaat cgaaagagaa ataaactaca attcaaattg
     1321 ttagctaatt tttcgattcc tgcagggaca ccagataccc ttcaaaccaa atctaccttc
     1381 atcccgtaaa cgatgggttt ggcagcacac ttgccctata tctactcatt tttactgcaa
     1441 caggttgaaa tctcagcact gtcagaaagc gctgatgact aaacagccca gggccgggcg
     1501 atgtaaccat cacacagaat cctgatagcg aaatacggcg tgactcgata cttcactccg
     1561 caatgcattc cttgatgaat tcgcaggcc
//
LOCUS       DAZFTN010000330         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFTN010000330 DAZFTN010000000
VERSION     DAZFTN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1587
                     /locus_tag="ACHKV2_005422"
     rRNA            <1..>1587
                     /locus_tag="ACHKV2_005422"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          402 a          355 c          498 g          332 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagtccctc
      481 gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg
      601 ttaattgatg gggtcagcgc aagcgaagct cttgatcgaa gccccggtaa acggcggccg
      661 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa
      721 tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga
      781 agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca
      841 ctgaacattg agccttgatg tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc
      901 tgcatggagc cgaccttgaa ataccaccct ttaatgtttg atgttctaac gtggacccgt
      961 gatccgggtt gcggacagtg tctggtgggt agtttgactg gggcggtctc ctcctaaaga
     1021 gtaacggagg agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg
     1081 gcataagcca gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt
     1141 gatccggtgg ttctgaatgg aagggccatc gctcaacgga taaaaggtac tccggggata
     1201 acaggctgat accgcccaag agttcatatc gacggcggtg tttggcacct cgatgtcggc
     1261 tcatcacatc ctggggctga agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg
     1321 tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg
     1381 gagaactgag gggggctgct cctagtacga gaggaccgga gtggacgcat cactggtgtt
     1441 cgggttgtca tgccaatggc actgcccggt agctaaatgc ggaagagata agtgctgaaa
     1501 gcatctaagc acgaaacttg ccccgagatg agttctccct gaccctttaa gggtcctgaa
     1561 ggaacgttga agacgacgac gttgata
//
LOCUS       DAZFTN010000331         1574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFTN010000331 DAZFTN010000000
VERSION     DAZFTN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1574
                     /locus_tag="ACHKV2_005423"
     CDS             <1..>1574
                     /locus_tag="ACHKV2_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6943089.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGV
                     RFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTF
                     RINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLG
                     TGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKL
                     HLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQKDT
                     NDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTT
                     PLFVAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAK"
BASE COUNT          372 a          413 c          478 g          311 t
ORIGIN      
        1 accctgcctg atggtgtgga gacatacggt gtatggggac tatccctgcc gtcactgcgc
       61 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      121 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      181 cagtcaggca ccctgaacag cgtcatccct ccggcagtgc agcacctgac ggtggaggtg
      241 agcgcagctg acggccagta tctggcgcag gtgaaatggg acacgccgcg ggtggtgaag
      301 ggcgtgcgct tcagtctgcg cctgaccagc ggaagcggag aaggcagccg tctggtgacc
      361 accgccatca ctgcggatac agagcatcgt tccagtggtc tgccgccggg ggaatacacc
      421 ctgacggtca gggcgattaa cagttatggc cagcaggggg aaccggccac caccacgttc
      481 aggattaatg cacctgcggt acccgccacg attgagctga caccgggcta ttttcagata
      541 acagcggtcc cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt ctggttttcg
      601 gagacaaaaa tcgcagatac atctcaggtg gaaacctctg cccgttatct ggggaccggc
      661 agtcagtgga gtgtatccgg cccgcacatt aagcccggga aggatttctg gttttacgtg
      721 cgcagcgtca acctggtggg gaaatctgct tttgtggaag ccagtggccg ggccagcaat
      781 gatgcagaag ggtatctggg gctgtttcgg gaaaaaatag gaaaactgca tctggctcag
      841 gggctgtggg agctgataga caacagccag cttgcggatg agatggcgga gatgaagacc
      901 tccatcaccg aaacccgcaa tgaaatcaca cagacggtca gtaaaacgct ggaggaccag
      961 agcgccatca tacagcagat acagcgcgtg cagaaggaca caaatgatga ccttgctgca
     1021 ctttacatgc tgaaggtaca gaaaacaaaa aatggcatac cctatgttgc cggtattgga
     1081 gcggggattg aggatactga tggccagccc ctgagcaaca tactgctgct ggctgaccgt
     1141 attgcgatga ttaacccgga ggacggcaac accacgccgt tatttgtggc gcaggggaat
     1201 cagttgttca tgaacgatgt gttcctgaaa cgactgtttg cggtgagtat cacgtcatcc
     1261 gccaatcccc cgacgttttc cctgacgccg gagggcaggc tgaccgcaag aaatgctgat
     1321 atcagcggta acgtgaatgc gaattccggg acgctcaaca acgtcacgat taacgagaac
     1381 tgtcgggttc tgggaaaact gtccgcgaac cagattgaag gcgatctcgt taaaacagtg
     1441 ggcaaagctt tcccccggga ttcccgtgca ccggagcggt ggccatcagg gaccattacc
     1501 gtcagggttt atgacgatca gccgtttgac cggcagattg ttattccggc ggtggcattc
     1561 agcggcgcta aaca
//
LOCUS       DAZFTN010000332         1568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFTN010000332 DAZFTN010000000
VERSION     DAZFTN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            107..583
                     /locus_tag="ACHKV2_005424"
     CDS             107..583
                     /locus_tag="ACHKV2_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6943090.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            580..>1568
                     /locus_tag="ACHKV2_005425"
     CDS             580..>1568
                     /locus_tag="ACHKV2_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6943091.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSR"
BASE COUNT          361 a          378 c          461 g          368 t
ORIGIN      
        1 aagatgatga ttgtggttgt tttttgttcg acatctttat atggcggcat ttctttacaa
       61 aaaaacagag tcacttctgt tctgcggttt atgtggaggg atgtaaatgg acggcgagct
      121 gaaaaatatg aagttaaata ttaatcaact ggcagccctt tcaggtctgc accggcagac
      181 tgttgccgcc agaatggcgg atgttcctct tgcaccaggc agtaatgaaa agaaaaaact
      241 gtatctcctg acggatttga taacttcgtt gctggaaaaa ccaccatctt ccgaagatga
      301 ggatatggat cctcacgctc gtaaggcatg gtatcagtcc gagcgcgagc gtcttaaatt
      361 tcagcatgaa actgttcagc ttgtgccagt cagtgatgtc aggcggtcct tttctgtcgt
      421 ggtgaaagcg atagttcagg tactggaaac ctggcctgac cggctggaga gggacagggg
      481 gtggactgca tcacaactga atgaagtaca gattgtggtt gatgagatcc gcgacacact
      541 ggaaaaggca gtcattgact gttgtgatga ggccgatatg tgaatcaggt gaacgagagc
      601 catagccgcg catccgatat ctggcgcgaa gtggcctcgc tgtttcgccc acccagccgg
      661 ttaccagtag cggaagccat caggcgttat atgcgggttc cacggggagc caatacttcc
      721 ggtccgtggg agtcatcgct gacgccctat atgattgacc ccattaatac attatcagcc
      781 cgtgaatatg acgcggtggt gtttgtggga cctgcgcgaa ccgggaaaac cgaagggctg
      841 attgatggct ggattgtgta cggcatcatc tgtgatccgg cggatatgct ggtggtgcag
      901 atgactgaga cgaaggcgcg tgagcattcc agaacgcgtc tttccaggac gtttcgccac
      961 agtccggagg tcagcaagcg cctcagtcct tcccgtaatg acaacaacgt ccacgataaa
     1021 atgtttcttg acggctcctt cctgaagatt ggctggccgt cgatcaccgt cttttcctct
     1081 tcggattacc gtcgtgtggc gctgacggat tatgaccgtt tccctgaaaa tgtggacggg
     1141 gaaggggatg ccttcacgct ggcctcaaag cgtaccacca cctttatgtc ctcggggatg
     1201 accctggtcg agagttcacc ggggcgggat atcaccgata ccaaatggcg ttgtggtggc
     1261 gcacatgagg caccgccaac aacggggatc ctgtcactgt ataaccgggg agaccgccgc
     1321 cggtggtact ggccgtgtcc gcactgcggg gaatattttc agccggtgat ggataacatg
     1381 accggatacc ggaataaccc ggattttgtg gctgccggtc aggctgcccg tctgatgtgt
     1441 ccgcattgtc gcgggctgat tgcccctgag cagaaacgcg aactgaataa ccaggggatc
     1501 tggcttcgtg aaggtgaacg ggcggcggcg gacggcagta tcaccggaac gccacgaaac
     1561 tcccggat
//
LOCUS       DAZFTN010000333         1538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFTN010000333 DAZFTN010000000
VERSION     DAZFTN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1538
                     /locus_tag="ACHKV2_005426"
     CDS             <1..>1538
                     /locus_tag="ACHKV2_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6943092.1"
                     /translation="EYGYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDS
                     TLTAWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVF
                     YTRIQHGEPLVESRYLYDPLGRRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLT
                     TVQTDTTRIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPA
                     ELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHR
                     GLPLALISEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRY
                     YDPLQGRYITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAW
                     DILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNL
                     FGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKALQ
                     DAGY"
BASE COUNT          400 a          369 c          495 g          274 t
ORIGIN      
        1 gggaatatgg ctacagcgcc acgggcaggc tggagagcgt gcgcaccctt gcatcagacc
       61 tggatatccg catcccgtat gcgaccgacc cggcgggaaa ccggctgccg gacccggagc
      121 tacacccgga cagcacgctc acggcgtggc cggataaccg catcgcggag gatgcgcact
      181 atgtctaccg acacgatgaa tacggcaggc tgacggagaa gacggaccgc atcccggcgg
      241 gtgtgatacg gacggacgac gagcggaccc accactacca ctacgacagc cagcaccgcc
      301 tggtgttcta cacgcggata cagcatggcg agccactggt cgagagccgc tacctctacg
      361 acccgctggg ccgccggacg gggaaacggg tgtggcgtcg cgggcgtgac ctgacgggat
      421 ggatgtcgct gtcgcggaaa ccggaggtga cgtggtacgg gtgggacggc gaccggctga
      481 cgacggtaca gaccgacacc acgcgtatcc agacggtata cgagccggga agcttcgcgc
      541 cgctcatccg catcgaaaca gacaacggtg agcgggagaa agcgcagcgc cgcagcctgg
      601 cggagaagct gcagcaggaa gggagcgagg acgggcacgg tgtggtattt ccggctgaac
      661 tggtgcggct gctggacaga ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa
      721 gccgggcgtg gcttgcgcag tgcgggctga cggtggagca actggccaga caggtggagc
      781 cggaatacac accggcgaga aaagttcatc tttaccactg cgaccaccgg ggcctgccgc
      841 tggcgctcat cagcgaagac ggcaatacgg cgtggagcgg ggagtatgat gaatggggca
      901 accagctgaa tgaggagaac ccgcatcacc tgcaccagcc gtaccggctg ccggggcagc
      961 agtatgataa ggagtcgggg ctgtactaca accggaaccg gtactacgat ccgttgcagg
     1021 ggcggtatat cactcaggac ccgatagggc tggagggggg atggagtctg tatgcgtatc
     1081 cgctgaatcc ggtgaatggt attgatccat tagggttaag tcccgcagat gtagcgctaa
     1141 taagaagaaa agatcaacta aaccatcaaa gagcatggga tatattatct gatacttatg
     1201 aagatatgaa gagattaaat ttaggtggga ctgatcaatt tttccattgt atggcatttt
     1261 gtcgagtgtc taaattaaat gacgctggtg ttagccgatc ggcgaaaggg ctgggttatg
     1321 aaaaagagat tagagattac gggttaaatc tgttcggtat gtacggcaga aaagtaaagc
     1381 tatcccattc tgaaatgatt gaagataata aaaaagactt ggctgtaaat gaccatgggt
     1441 tgacatgtcc atcaacaaca gattgctcag atagatgtag tgattatatt aatccagagc
     1501 ataaagaaac gataaaggct ttacaagatg ctggctat
//
LOCUS       DAZFTN010000334         1494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFTN010000334 DAZFTN010000000
VERSION     DAZFTN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..376
                     /gene="escR"
                     /locus_tag="ACHKV2_005427"
     CDS             <1..376
                     /gene="escR"
                     /locus_tag="ACHKV2_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO6943093.1"
                     /translation="FFQKVDEKILSPYRGFLEKNTEKDNVEFFERAAQKKLGNETILK
                     KDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPF
                     KILLFILVGGWQKLFEFLLVVN"
     gene            376..645
                     /gene="escS"
                     /locus_tag="ACHKV2_005428"
     CDS             376..645
                     /gene="escS"
                     /locus_tag="ACHKV2_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO6943094.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            645..1421
                     /gene="escT"
                     /locus_tag="ACHKV2_005429"
     CDS             645..1421
                     /gene="escT"
                     /locus_tag="ACHKV2_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO6943095.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1414..>1494
                     /locus_tag="ACHKV2_005430"
     CDS             1414..>1494
                     /locus_tag="ACHKV2_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO6943096.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEE"
BASE COUNT          448 a          248 c          268 g          530 t
ORIGIN      
        1 tttttttcaa aaagttgatg agaaaatatt atcaccatat cgcggttttt tagaaaaaaa
       61 tacagagaaa gacaatgtag agttttttga acgtgcagct caaaaaaaat tgggtaatga
      121 aactatctta aaaaaagact ctctatttat actgttaccg gctttcacga tggggcagct
      181 tgaagctgca ttcaagatag gttttttgct ctatttaccc tttattgcga tagatttgat
      241 catttccaat atcttattgg ccttgggtat gatgatggta tcgccagtaa caatttcgat
      301 tccttttaag atcttattat tcattcttgt tggaggctgg caaaaattat tcgaatttct
      361 gttggtggtg aattgatgga tactggatat tttgttcaat tatgtgtgca aacgttctgg
      421 ataatattta tcctctcatt gcctacagtc atagcggcct ctgttatcgg tattattatt
      481 agtctggtcc aggctataac gcagttacag gatcaaacat tgcctttttt gctaaaaata
      541 atagcagtgt ttgctacgct tgccctgact tatcactgga tgggaacaac aatcatcaac
      601 ttctcgtcga taatatttga aatgattccg aaggtgaacg gctaatgaat gagataatga
      661 cggtcatagt atcatcattt tattgtatac tcaggccgct ggggatgttt attatcctac
      721 caatattttc aacaggtgtt ttactcagta actttattcg caactcaatt atgattgcgt
      781 ttacgcttcc gatcatagta gagaactata cattttcgga aaaattgcca tctggaatct
      841 ttcagttgac aggaatagcg ctaaaagaaa taagtattgg ttttttcatt gggttatcat
      901 ttactattct tttttgggca atagatgcgg ctggacagat tattgatact ctaagaggct
      961 caacaatatc ttcaattttt aacccgtcca taagtgattc atcttctatc actggcgtta
     1021 tcttgtacca atttatctct gtgatctttg ttattcatgg tgggatacaa agcatcctag
     1081 ataagctata tttatcctac gagatattac cattacaagc cgatattgca ttcaatcgtg
     1141 ctttaataga ttttttgttt tctctatggg attcatttat taaactgatg ttatcatttt
     1201 cagttcccat gattatcggt atattcttat gtgatatggg gtttgggttt cttaacaaaa
     1261 cagcacctca gctaaacgta ttcacattat cactgccagt aaaaagtttg atagcaatct
     1321 ttatattgct attagttatt catgtttttc ctgattttat tacggcaaat attcattctg
     1381 acataattga tagatcgtta ccgagcatga ttaatgagtg aaaaaacaga aaagcccaca
     1441 cccaaaaaac taagggatct aaaaaagaag ggcgatgtaa caaaaagtga agag
//
LOCUS       DAZFTN010000335         1488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFTN010000335 DAZFTN010000000
VERSION     DAZFTN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..441
                     /locus_tag="ACHKV2_005431"
     CDS             <1..441
                     /locus_tag="ACHKV2_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943097.1"
                     /translation="TNTYLLGRVIFLYNSMIYKFINCQEHETNNIHSAMINNLLQEVD
                     IALGKINDIIDSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITF
                     LIHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
     gene            complement(628..>1488)
                     /locus_tag="ACHKV2_005432"
     CDS             complement(628..>1488)
                     /locus_tag="ACHKV2_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6943098.1"
                     /translation="GWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVW
                     LTLSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQL
                     PLFVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKA
                     RIQHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGR
                     VATAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWA
                     DYVMSQIVNK"
BASE COUNT          455 a          350 c          277 g          406 t
ORIGIN      
        1 acaaacacat atttattagg aagagtaata tttctgtata actcaatgat ttataagttc
       61 ataaactgtc aggaacatga aaccaataac attcactcag ctatgataaa taacctatta
      121 caggaagttg atatagccct tggtaaaata aatgacatta tagacagcag aaacatatca
      181 gccccccatg aactggcaaa tattcttacc cgcgaaaaaa tacttacaac acgggaaaaa
      241 aaaggaaacc tgataagcct gtttgatgga ttcactttat tccattgtgt tggaatgata
      301 acctttctta tccattatct cagaacacct gaagaaaaag ttgaaaatat atttatgtta
      361 tatggtgcag ataaaaacaa taaactacgc agaagactga tttatgacgc actaggaata
      421 attcagtctc agcaggagtg aagagttaaa caggcaaatt attttttata aaaaggggat
      481 tgttaagtaa tcccctgtta atcaaaatac gctttcctga cgatctgata attatcagct
      541 caatacattt gacataaaag cggtttctta attactactg ctacgatcac atatcaaatc
      601 atgatcgtac aatatacatt ttcactatta tttattcaca atttgagaca tcacataatc
      661 cgcccatgcc tgcatcatcg gtacacgctg ctccagtaga tcagtacgat gatatgccgc
      721 ctcaacctta tttttcagcg tatgagcgag cgccctttcc gccagatccc gcgaataccc
      781 ctgttcgcta caccagtccc tgaatgttga gcgaaaacca tgtgccgtgg caactcgccc
      841 cggaatgtca ctgacggctt tctttttacg cagaaaactt gtcaacacca tatcggaaag
      901 gatctgctgc tttctgggtg aagggaacac cagttcatca tgcaggccac gtatattttc
      961 cagaatgtaa atagcctgcc gggataaagg aacacgatgc tgtatcctgg ctttcattct
     1021 ttctgcaggt atagtccata cccgcttatg aaaatcaatt tcagcccagc gcattcccct
     1081 ggcttcgccc gagcgagttg ctgtaagtat caccattaat aacagtgcgc gggtaacatt
     1141 ataaggttca tcggtataca cactggtcgc cacaaaaagc ggtaactgcc tccagggcat
     1201 tgcgggttgg tgttcatcac gtcctcttgt ctgctgagga agcaaatggt caaccacatc
     1261 aacaggattt gctacacaaa aaccgtgcgc ccatccccac tgcataacaa catgaatgcg
     1321 ctgtttaacc cggcttgccg tttctgacaa ggttaaccag actggacgca gtgtttctgc
     1381 cacgtccgca gccgtaatcg aatccagcgt ttttgctccc agttgaggaa acgcgtaatt
     1441 ctcaagcgtc gataaccact gccttacatg ctttggattt tcccatcc
//
LOCUS       DAZFTN010000336         1468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFTN010000336 DAZFTN010000000
VERSION     DAZFTN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            201..305
                     /locus_tag="ACHKV2_005433"
                     /pseudo
     CDS             201..305
                     /locus_tag="ACHKV2_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(425..1444)
                     /gene="nleC"
                     /locus_tag="ACHKV2_005434"
     CDS             complement(425..1444)
                     /gene="nleC"
                     /locus_tag="ACHKV2_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO6943099.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
BASE COUNT          402 a          319 c          285 g          462 t
ORIGIN      
        1 agcctttaat tatcagtttt atttattttc cagcaattga ttaatcaatt gtttatatac
       61 aaattattcc ttttctttct ctcacgcaaa attcacaaaa tattgttgct gcaatggacc
      121 tgtacaatag tgaatcacgg tacttttctg tgcacaacgt actgcagttt ctttacgatt
      181 tatagctgta ctgttgaatt atgtacaatt tgaatccatg catgacgtgt gatgcctatt
      241 gtgcattttt ccgagcctct ttttactggg ccgaagccga tgatgctgat agtctcgttc
      301 cggccatgtt cacttactac agtaaaatta ggggaattgc ctttacatac aatgttgcac
      361 gattacccat catcaactcc gttaaaatgt atgaatagta accttatgtc actgcaaaga
      421 cgaatcattg ctgattgtgt ttgtccacat ccccaaatgt aaaaatataa ggggcgccat
      481 gatacagttg gatctgattt gcatcctgta aattatcgtt gatagccaat ccaggaaaat
      541 catgttgctg gataaatccg tatcctatgt tagacacagc ggaatactct gtgaaatcag
      601 gactcgcctc atatcggtca ctgatcgtac ccagcctttc gaagaaagcc ctctcattct
      661 cttcatgcct catggcagcc tgtcgaaggg cattcaggtt acgtagccta agatgagctt
      721 cacgttctgg ctctgcatat cctttgaagt cgggaacact ccatcccagt tcttgagcga
      781 cacgacgtgc gagaatctcg gtgggtccta actctatatt actatctcca gatggatcgc
      841 tagatccagt aacatgatga ataatctcgt gaatcagtcc ttcctgccac gatggcatct
      901 cataagagtc agtatctggc gcaacactaa aactgacata aggctcttcg ttatcgtttt
      961 ctcctgcttc gcaaatgggg aaaattggct cttgcccgac atcatattca tataattcgt
     1021 tacaggttag tgattgaata tcatcaattt tgacagaaga ttcttcgtta agctcgtatt
     1081 cgtttctgta tttaatgcaa ccaatgtgta ccttctcatt atgaatgcca tagcttacgg
     1141 catcacgaaa tgtctgtgat cggctcaaag catcaagtac agtgttgccg atcatatcga
     1201 ctgtatgctg atcgataagc ctgctacggc tgtcatggac ggcgcgtatt acactttcgt
     1261 atacgttgct taaatctgct gcggaaagtg gtattcgctt acctatatcc aaaacgtaat
     1321 ccgcataggc attttcagca cgattaggag caatgggagc agagtatcct gccggggcgg
     1381 aaaagttgaa gttggactgt aatgagggaa ttttcatgtt tatatctaat accctgcaaa
     1441 tcatttatta caacaaaata aactatat
//
LOCUS       DAZFTN010000337         1463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFTN010000337 DAZFTN010000000
VERSION     DAZFTN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..176
                     /locus_tag="ACHKV2_005435"
     CDS             <1..176
                     /locus_tag="ACHKV2_005435"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6943100.1"
                     /translation="RDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQPVSGGRLGV
                     KIYPIMLEGQVKK"
     gene            173..994
                     /locus_tag="ACHKV2_005436"
     CDS             173..994
                     /locus_tag="ACHKV2_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6943101.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1232..1463
                     /locus_tag="ACHKV2_005437"
                     /pseudo
     CDS             1232..1463
                     /locus_tag="ACHKV2_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
BASE COUNT          394 a          288 c          418 g          363 t
ORIGIN      
        1 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
       61 taatggacga tgagcagttt gatgaaatca atatcgttcg tgctcagcca gtatctggtg
      121 gacgtctggg ggtgaagatt taccccataa tgcttgaagg gcaggtcaaa aaatgaaact
      181 ggaagattta ccgaaatact actccccaaa atcccccggc ctgactgatg catcggcctc
      241 aacgtcgaaa gatacgctga gtatcactga tgtgatggcc gcgcagggca tgacacagaa
      301 ttgggctgag atggggtttt ctgcgttcct tgggaaaatg ggcattagta tgaatgacag
      361 agagcgggca acagaattgc tgacagaata tgcactcagt cggtgtgatc gcgtggcggc
      421 gttaagaaaa ctcccggcag aaataaaacc ggcagtgatg cgtattatgg cttcgtatgc
      481 gtttgaagat tatgcccgta gcgcggcgag caaaaaacag tgcccctgtt gtcacggaaa
      541 aaaatttatt gaaagcgagg tttttacaaa caagatccag tatccggatg gtaagccgcc
      601 agtgtgggca aagtgcacaa aaggcgtgta tccgtcttac tgggaggaat ggaaaaaagt
      661 cagggaggtg gtaaaagttg cctgtccgga gtgtggaggg aagggggagg tttccaccgc
      721 ctgtaaagat tttcgtgggc gcggtgttgc cattcatcgt gaagagtcgg taaaacgtgg
      781 tatgcctgtt atcagagact gccagcgttg tggtggtcgt ggctatgaaa gattaccttc
      841 aacggaggca tttaatgcca tatgtaatgt aaccgatgcc atatctcttg atacatggaa
      901 aaaaacagtt aaacgttttt acgatacgct ggtggtgcag tttgatattg aagaagcatg
      961 ggcagaacaa caactgaaaa aggtgaccag atagctttgt tgatttttcc cgaatctgtg
     1021 gtaaatttgc cctaacgatg ggcgttttat gcctgacgtt agaagatttt ttacacccgt
     1081 cgccaggcgg gtttttttat gactgaaatc acgccagtac agtaaacgcg ctggtggttg
     1141 tgaataccgg tctttctgct tgctggcttt ttcgacaaga gttattggtg tgtcacgtta
     1201 accggaaaaa ggaaagtttg agaaacgcga tctggcacag gcggtgatta acgccgcata
     1261 cctggtggcc tgtgcagatg gtgaatgtga ggcttcctag aaagcgaaga tcgaacaggt
     1321 actgcgtaat cagccagcgc tgtccgcgtt tacgtcagaa attaatgcga ttagcgcaac
     1381 cattatcggt cagctggata cgaactttaa aactggtcgt cgtgcggcgt tacgtgagat
     1441 cgaggatgtg aaacacgata cgc
//
LOCUS       DAZFTN010000338         1459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFTN010000338 DAZFTN010000000
VERSION     DAZFTN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(240..395)
                     /gene="hokD"
                     /locus_tag="ACHKV2_005438"
     CDS             complement(240..395)
                     /gene="hokD"
                     /locus_tag="ACHKV2_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6943102.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            1000..>1459
                     /locus_tag="ACHKV2_005439"
     CDS             1000..>1459
                     /locus_tag="ACHKV2_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6943103.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCL"
BASE COUNT          416 a          317 c          311 g          415 t
ORIGIN      
        1 aaaacaccgt ccgcgcctgc tggcaaagac ggcagaatca tcagaattgt ataatttggt
       61 atcgtgcgcc atcggttgtc tctgctggcg cagcaggtgc cagttgttca ggctggcgtg
      121 cgaattgtaa accagaatgc caggaaaaaa caaaacccgc cgaagcgggt taagtgcggg
      181 tgcgttgagg atgcctgatt catcagaggt ggcgagggat ttctcccccg ccaggtctct
      241 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggactcgt
      301 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
      361 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc
      421 acgtaagagg ctaacctaga tttgccgttc atagattgag cctcagatta atgttaggcg
      481 tcttgcagga cgcgtaatgt taactggggc ttttctctat ctgccgttgg tgttcatgcc
      541 cgaggcagat agcctcaagc acccgcagct attctactta acttcccatt acctcgccaa
      601 tatgaaatcg gtcagaaaga cagacgtccc ataaggacaa caacggaaaa agagattttg
      661 cttgatgcta tttacctctg caccatgaag ttgcgccaaa atcccagtgg tgataacagg
      721 aatttggttg ttggtgattg gggaaagaag tacgttagac tgtgggttag ttcattgaca
      781 tgatagttac ctgccttcaa tttcctaagc aagcgatgta aacgataatc aatatcaaat
      841 gtattgacag gtaaaataat aacgatgtac ctttgctgat aacatatatc agtcttaaat
      901 cataatggag gtagataaaa taactaaaca cacacaacaa attttttatc agtgacatag
      961 catatcttaa gcatccttta atgctcatgg ggagaacgca tgactaaaca tatggacatt
     1021 cattgtcaaa cacgcatatt taactcaatt aaccatgaca ccaaatataa aatatgtatt
     1081 tttactccaa aaaacattaa aaaaaataat gcaactcttt atatacttga tgggaatagt
     1141 gccaacaatt acatttctga tattctgcct gttattgatg cactacccaa tccaccagtg
     1201 ttagttacac ttggttatga atcttggaat aaccttagca ttcatcgccg tgcttacgat
     1261 tatactcctg atggtgaaaa tgctattgtt gataactcta aaccagcatg gatctacttt
     1321 actggtggag gaagccagtc tttccgagaa ttattactaa ctcagatcat gccatgggtt
     1381 agtactattg ctccgaacag ttccagaatc ggtatatggg gacactcgct aggtgctatt
     1441 tttgtgcttg attgcttaa
//
LOCUS       DAZFTN010000339         1428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFTN010000339 DAZFTN010000000
VERSION     DAZFTN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1428
                     /locus_tag="ACHKV2_005440"
     CDS             <1..>1428
                     /locus_tag="ACHKV2_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6943104.1"
                     /translation="SGLPLGEYTLTVRAINSYGQQGEPAITTFRINAPAKPATIELTP
                     GYFQITAVPVLAVYDPTVQFEFWFSEKRITNTAQVEKSARYLGSGSQWTVQGSRIKPG
                     TDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQL
                     ADEMAEMKTTITETRNEITQTVSKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKT
                     KDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDV
                     FLKRLFAVSITSSGNPPTFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHEN
                     FNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSS
                     SGANNWTPTTSISDLLVVVMKKSTAG"
BASE COUNT          386 a          342 c          410 g          290 t
ORIGIN      
        1 cagtggtctg ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca
       61 gcagggggaa ccggccatca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat
      121 tgaactgacg ccggggtatt ttcagataac ggcggtaccg gtgctggcgg tgtatgaccc
      181 gacggtgcag tttgagttct ggttttcgga aaaacgcatc acgaacacgg cacaggtgga
      241 aaaatctgcc cgttatctgg ggagcggcag tcagtggact gtccagggaa gccggattaa
      301 gccggggacg gatttctggt tttacgtgcg cagcgtcaac ctggtgggga aatctgcgtt
      361 tgtggaagtc agcgggcagc ccagcaatga tggtgaaggg tatctggaat ttttccggga
      421 aaaaatagga aaactgcatc tggctcaggg gctatgggag ctgatagaca acagccagct
      481 tgcggatgag atggcggaga tgaagaccac catcacggaa acccgcaatg aaatcacaca
      541 gacggtcagt aaaacgctgg agaaccagag cgccactata cagcagatac agcgcgtgca
      601 gaaggacaca aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga
      661 cggcattccc tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact
      721 gagcaacata ctgctgctgg ctgaccgtat cgcgatgata aatccggaga gcggcaacag
      781 cacgccgtta tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg
      841 actgtttgcg gtgagtatca cctcgtccgg caatcccccg acgttttccc tgacgccgga
      901 cgggcgactg acggcgaaaa atgcggatat cagtggcagt gtgaatgcga actcagggac
      961 gctcaacaac gtcacgatta atgagaactg tcagattaag gggaaactgt cagccaacca
     1021 gattgaaggc gatattgtca aaacggtcag caagtctttc ccccgcacga gcacttatgc
     1081 cagtggcacc atcacggtaa gaatcagtga tgatcagaag tttgaccggc aggtcatgat
     1141 accgccagtg ttattccgcg gtggtaagca tgagaatttc aacagtaata accaacagtc
     1201 atactggtat tcaacctgcc ggttaagagt gacccgcaat ggtcaggaga tttttaatca
     1261 gtccacgacg gatgctcagg gcgtattttc ctcagttata gatatgcctg ccggacaggg
     1321 gacgctgaca ctgacattca ccgtatcttc atcaggagcg aataactgga caccaacaac
     1381 cagtatcagc gatctgctgg ttgtggtgat gaaaaaatcc acagcagg
//
LOCUS       DAZFTN010000340         1373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFTN010000340 DAZFTN010000000
VERSION     DAZFTN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            527..718
                     /locus_tag="ACHKV2_005441"
     CDS             527..718
                     /locus_tag="ACHKV2_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943105.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(788..928)
                     /gene="ykgO"
                     /locus_tag="ACHKV2_005442"
     CDS             complement(788..928)
                     /gene="ykgO"
                     /locus_tag="ACHKV2_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO6943106.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(928..1191)
                     /gene="ykgM"
                     /locus_tag="ACHKV2_005443"
     CDS             complement(928..1191)
                     /gene="ykgM"
                     /locus_tag="ACHKV2_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6943107.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          405 a          280 c          300 g          388 t
ORIGIN      
        1 ttcataaggt tattggcaat ataacgttca attcttactt gcattttata ttagccagga
       61 actttgccct gaggtgttag gaatagtctt aatgtgttgt gtaatggact agtaaaaaaa
      121 caagtctaaa tgataagaaa tagaatatga aactatcatg agtcataatt aaagatgatt
      181 tgccaatcag taaggtgttt taatggatta acatgccgca caaaatggtt atgggtgttc
      241 atcccaataa gtgatgaaaa attaaacagg tgtaagtacg tcatctgtcc ggtaagcccc
      301 ctaagagcca gattaagtga agttaaacga agactgccag ttgtcaccgg aacgatggcc
      361 tctttgccag ggcactgcat tcagtcatct gtgcgcgtta ctctaactgg cactcgaacg
      421 tcggcgacgg ggttaccggt ggatcaggcc gttcctgtgg cgtaaacgtt tttatgtcag
      481 taccaacaga tttaccacat ttatcatctt aacagtttga gtgtaaatgc aggctgcgtc
      541 gcaaagggag gaaactgagt ggcgtgttca gtctaaaaga gggttgatgc cagcatacag
      601 gggcgaagct ggtcaacaag tcaacattaa tataatggaa tattcggaga gaaacgtcag
      661 gcagttgacc ttaaatgaac aggaagatac atccccagga aaattaatgc tggtgtaata
      721 aatacaccaa cattaatccg gtctgattat taatcagaca aatatcgtta attcgtcgaa
      781 ttttaaatca acgttttttc ttacgaccct gaacggcctt aaagcgtgga ttagatttac
      841 aaatcacata tagccgtcct tttcgcttca caatctgaca gtctggatgg cgttcttttg
      901 cggtacgcag agagttaagg actttcatca cgcccccttt ttcgtgctaa caaaacgacc
      961 aaaacgttgg gtgaatcgtg caacatttcc ttctgatgcc actgttctca gcttccctgt
     1021 atagaacggg tgcgatttcg aagagacatc aattgttacg tacggatacg ttacgccatc
     1081 cagctcaatc tcacggtctg ttttgatagt cgagccgatt ttaaagtact catcaacact
     1141 ggtgtcgtgg aacaccacag tacgatactc aggatggata ttgggcttca tcatttttac
     1201 ctgttatgtt ataacataac cacaaagata catgctcatc cggatcattt caacacccaa
     1261 tccctgtgac cattagtggt atgtggaatt tcctcaacgg ggaagtctgg cattgttaac
     1321 agccattggc gacgctccca taaacgcctg gatgagtgat tggtaagcgt aca
//
LOCUS       DAZFTN010000341         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFTN010000341 DAZFTN010000000
VERSION     DAZFTN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..679
                     /gene="espJ"
                     /locus_tag="ACHKV2_005444"
     CDS             26..679
                     /gene="espJ"
                     /locus_tag="ACHKV2_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFO6943108.1"
                     /translation="MSIIKNCLSLINNALNIQKTSYSLTKMEQAGKLLNRKITPENTP
                     PMLLSYRNADLTQEKNITERVLSIFKIKRDFVAVRIQNNQFTDLKNKKIQGHQNTVAS
                     VMDWYNPQKNALGITMGTPRKSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQKS
                     YSKTEDSSWVAASVGSLLDKGAKVYPDTSCSLRLGEPFIFTLPESVRVDVDIYPLKK"
     gene            1004..>1365
                     /gene="tccP"
                     /locus_tag="ACHKV2_005445"
                     /gene_synonym="espF(U)"
     CDS             1004..>1365
                     /gene="tccP"
                     /locus_tag="ACHKV2_005445"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFO6943109.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQ"
BASE COUNT          502 a          271 c          223 g          369 t
ORIGIN      
        1 tttatatgag aaaaaggcat ttataatgtc aattataaaa aactgcttat cattaattaa
       61 caatgcatta aatatacaaa aaacatcata ttctctgacc aaaatggagc aggcagggaa
      121 gttgttaaat agaaaaataa ctccagaaaa tactcctcca atgttgttat catacagaaa
      181 tgcagattta acgcaagaaa aaaacattac agaaagagtt ttatccatat ttaaaataaa
      241 acgagatttt gttgctgtaa gaatccaaaa caatcagttt actgatttga aaaacaaaaa
      301 aattcaaggc catcaaaaca cagttgccag tgtcatggat tggtataatc cccaaaaaaa
      361 tgctttgggt ataactatgg gtacaccaag aaaatcggcc gatatagcaa aagaagagca
      421 cagaaatgca ctaaatttca tgataatgga aaaaaacaca tttcatgaaa aaatcttaaa
      481 cagcaatgac aatttgcaaa aatcatacag caaaacagaa gacagtagtt gggtcgcagc
      541 ttctgtcggc tctcttctag ataaaggagc aaaagtatat cccgatacat catgctctct
      601 gagactggga gaaccattca tctttacgct gccagaatca gttagagtcg atgttgacat
      661 atatcctctc aaaaaataat cttaaaggta ttgacatata agaaataatt aagtgtattc
      721 acattagact agtcgcttaa gagtagattt ttggcatttt gggaagatca gcgggacata
      781 agagacatac aacacaggag gaatggctgt tttttaatag tgctttgaat tccgcatgaa
      841 acctttctta gcagtcgaaa agcaaaacat acgttttctt tttaacaata attgatgagt
      901 attgcatcga gtgtcattta tacatcgccg catttatttg ccactataaa aaggctgatt
      961 acatatctat cggagaacta aaattactta taaggtaatc tgcatgatta acaatgtttc
     1021 ttcacttttt ccaaccgtca accgcaatat tacagctgta tataaaaaaa gcagcttctc
     1081 tgtatcacca cagaaaatca cattaaatcc tgtaaaaatc agctcacctt tttcaccaag
     1141 cagtagctcc atcagcgcaa caactctctt tcgagcccca aacgcccatt cggcatcatt
     1201 tcatcgacag tctactgctg aaagttcgtt acatcaacaa cttcctaatg tgaggcagcg
     1261 cctgatacaa catcttgcag agcatggcat taaacctgcc cggagtatgg ctgaacatat
     1321 tcctccggca cctaactggc ctgcgccacc accgccagta caaaa
//
LOCUS       DAZFTN010000342         1336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFTN010000342 DAZFTN010000000
VERSION     DAZFTN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..580)
                     /gene="ybfC"
                     /locus_tag="ACHKV2_005446"
     CDS             complement(38..580)
                     /gene="ybfC"
                     /locus_tag="ACHKV2_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO6943110.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLPNK
                     DESYECLTKIQHELLRSEEK"
     gene            complement(605..>1336)
                     /locus_tag="ACHKV2_005447"
     CDS             complement(605..>1336)
                     /locus_tag="ACHKV2_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6943111.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          336 a          300 c          258 g          442 t
ORIGIN      
        1 tttccgaatg gttaatttat tttctcagga agatgtttta tttttcttca ctacgaagca
       61 gttcatgctg tatttttgtt aaacattcgt aagactcatc tttgttcggg agatgtgctg
      121 tcattcgagc ataagttggc ttctcatctt taaaatattt aaccatgatt gaattatcaa
      181 ggactgccaa caactgagct ggatcactgc tctttttttg atagataccc gctcttatat
      241 aacttctaaa ccgatcgccg agaaacgccg ttttttctgc tgtatttatc aactcctgca
      301 acgttccttt gatggcatga ttttcattaa ggcgtagtga cccgacacga attaagagtc
      361 tcgccccgct tggtgtgaat ttattatttt tacatgtacc cagttctgtt tgccaataat
      421 actcaccgat gtcctcattc tgataccatc tccacatggc tttattattt aagacaggaa
      481 acgcggaaaa aatagtaccc ttagcgaaaa tagtaaaaca atcatgatct ttagcttgta
      541 tttcgcaatc agctataaca ccaaaactaa caaatatcat cagcaaaaaa aaacaaaact
      601 cttttcattt tcttattcct ctcgatgttc tctgtttcat tttagctaaa atttcaccca
      661 aatcatctat ccctgctgtt gctggcccat cgggtttatt aatgtcaggg aataaatagg
      721 gttctgcaca agtatatcct ggtggtaatt gagttgttgg ccaatttgtc tgtggcaggt
      781 aatcacttgc tgtctttgta tccatccctg gatactgggg atcatcctgt ggcgtacaag
      841 tcagatttgg ttttacccac ttcctgcaat acagtgtgta gtcgtaaggg cttaatgctc
      901 tgaagaatgt tttgaaaaca ttaggcgcac tactggcctg gtcaatagga tatggcagtc
      961 ttaattgttt cgctatgtct ggaaatttac ttggatcggg cgtaggcggt ttgttgggtc
     1021 tcgctccata tccgccatta tttaagtgtc catattttga tgccagtccc attgaatcta
     1081 tatactgaac cggattcaac ggatactgat aaaaattcca tccccccttc agtccaatcg
     1141 gatcctgagt gatatacctc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
     1201 ggccggactc ctcatcatac tgctgccccg gcaggcggat aagctgctgc agctgatgcg
     1261 ggttctcttc attcagcagg ttgccccact catcgtattc tgcgcaccat tctgttgccc
     1321 cttccttact gataag
//
LOCUS       DAZFTN010000343         1318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFTN010000343 DAZFTN010000000
VERSION     DAZFTN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            36..>1318
                     /locus_tag="ACHKV2_005448"
     CDS             36..>1318
                     /locus_tag="ACHKV2_005448"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6943112.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPVEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRG"
BASE COUNT          295 a          349 c          365 g          309 t
ORIGIN      
        1 ttttattgcc ttcaaataaa tttaaggagt tcgaaatgga ccagaagctg ttaacggatt
       61 tccgctcaga actactcgat tcacgttttg gcgcaaaggc catttctact atcgcggagt
      121 caaaacgatt tccgctgcac gaaatgcgcg atgatgtcgc attccagatt atcaatgatg
      181 aattatatct tgatggcaac gctcgtcaga acctggccac tttctgccag acctgggacg
      241 acgaaaacgt ccataaattg atggatttgt cgatcaataa aaactggatc gacaaagaag
      301 aatatccgca atccgcagcc atcgacctgc gttgcgtaaa catggttgcc gatctgtggc
      361 atgcgcctgc gccgaaaaat ggtcaggccg ttggcaccaa caccattggt tcttccgagg
      421 cttgtatgct tggcgggatg gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag
      481 gcaaaccaac ggataaacca aacctggtgt gcggtccggt gcaaatctgc tggcataaat
      541 tcgctcgcta ctgggatgtg gagctgcgcg agatccctat gcgccccggt cagttgttta
      601 tggacccgaa acgcatgatt gaagcctgcg acgaaaacac catcggcgtg gtgccgactt
      661 tcggcgtgac ctacaccggt aactatgagt tcccgcaacc gctgcacgat gcactggata
      721 aattccaggc cgacaccggt atcgacatcg acatgcacat cgacgctgcc agcggtggct
      781 tcctggcacc gttcgtcgcc ccggatatcg tctgggactt ccgcctgccg cgtgtgaaat
      841 cgatcagtgc ttcaggccat aaattcggtc tggctccgct gggctgcggc tgggtgatct
      901 ggcgtgatga agaagcgctg ccgcaggaac tggtgttcaa cgttgactac ctcggtggtc
      961 agattggtac tttcgccatc aacttctccc gcccggcggg tcaggtgatt gcgcagtatt
     1021 atgaattcct gcgcctcggt cgtgaaggct ataccaaagt acagaacgcc tcttaccagg
     1081 ttgccgctta tctggcggat gaaatcgcca aactggggcc gtatgagttc atctgtacgg
     1141 gtcgtccggt cgaaggcatc ccggcggttt gcttcaaact gaaagagggt gaagatccgg
     1201 gatacaccct gtacgacctc tctgaacgtc tgcgtctgcg cggctggcag gttccggcct
     1261 tcactctcgg cggtgaagcc accgacatcg tggtgatgcg cattatgtgt cgtcgcgg
//
LOCUS       DAZFTN010000344         1315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFTN010000344 DAZFTN010000000
VERSION     DAZFTN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..730
                     /gene="ais"
                     /locus_tag="ACHKV2_005449"
     CDS             128..730
                     /gene="ais"
                     /locus_tag="ACHKV2_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO6943113.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKV2_005450"
     CDS             complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKV2_005450"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO6943114.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          359 a          286 c          283 g          387 t
ORIGIN      
        1 aaaattttga ggcattttgc cagcatcgta ggatttttaa tattacacca actgctaatt
       61 atcgccagaa atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg
      121 agtttacgtg ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt
      181 actggcgctc gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt
      241 gccacgtatc gacaataaaa cgctgggcag actggcacag cagcacccgg ttgtcgtttt
      301 gtttcgccat gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg
      361 tattacggtt aaaggtaccc aggatgcccg tgaactgggc aacgctttta gtgctgatat
      421 ccctgatttc gatctttatt ccagtaatac cgtccggacc attcagtccg cgacctggtt
      481 ttcagcgggt aaaaaattga cggtagataa acgacttctt cagtgcggta atgagattta
      541 tagtgcgatt aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca
      601 taatcattgc ctgacatata ttgctaaaga taagcgtgat gcgacattta aacctgatta
      661 tctggatggt ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt
      721 taatcactaa attttcaatc tgacagccag taatggctgt catcattgtt acagaagacc
      781 tttcaaacgt aacgtttttc gggtggcgac attcaaatca tagtgcgcca gatcttcagg
      841 ttttacccac gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac
      901 gcaatcaaaa atcaggtaaa tcatataaat ttcttccttg cgaccatctg catacgtctt
      961 ggtgcgaata tcatcgctga aggtccaggg cgtgatttct gtcaaaagca gctgttctcc
     1021 cagttcttcg cgaatttcgc ggcgtagagc ctcttcaatt cgttcgccgg gctccacgcc
     1081 gccacccgaa agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag
     1141 caaataagca ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt
     1201 attccttata aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga
     1261 cagtaatctc ttttgcgatg ataactgcac ttattattat ttttgtgtgt atcgg
//
LOCUS       DAZFTN010000345         1312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFTN010000345 DAZFTN010000000
VERSION     DAZFTN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(40..>1312)
                     /gene="ydfJ"
                     /locus_tag="ACHKV2_005451"
     CDS             complement(40..>1312)
                     /gene="ydfJ"
                     /locus_tag="ACHKV2_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO6943115.1"
                     /translation="LYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFI
                     FGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGA
                     EISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWR
                     IPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLR
                     FGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRI
                     PYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFG
                     CKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKM
                     PEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
BASE COUNT          385 a          335 c          282 g          310 t
ORIGIN      
        1 ataaaaatcc cctctccggt aagagaaggg attgaggatt tacagacttc tggaaggttg
       61 cgcagctctt acaacacgcg gttgatcttc cgcagcgtct tccagcgcac ttaaatcacg
      121 gtctttcact tctggcattt tcagcgcaga gattaaacca atcactgaat atgccatgat
      181 cataatggcg atcggatacc aggattccgt catggtgcag aaaatacccg ccaggatagg
      241 accaaaaccg gaagcgataa gaccaccaat ttctttagaa atagccatcc gggtaaagcg
      301 gtttttacag ccgaacattt ctgccatggt aatgttttcc agagcaaata atcccagcac
      361 cgcacagtta tgaatcacaa tcagtgcaac cataatggtg ctcggggcat agcttttatc
      421 tacgatgata gaaagcattg gccatgccag cacaatcgcg gaggtattca taataatata
      481 cgggatccgg cgaccaattt tatcggataa ccaaccaagg aacggaatgg tcataaagcc
      541 gggaatcgaa ctgatcatca atgcatctgt tggaattgct ttgttaaaca ataacgtctg
      601 tactaaatag cctgcaagga aagtctgaat taacccggag ttacctgcct gaccaaaacg
      661 cagccctgtt gccagccaga aggatttgct ctggaatatg ctaccagcag gtgcaggttt
      721 tgctgtcggt tggttgctgt cgttaacctt ctcaaagacc gggctttctt tcagattcat
      781 acgcaaccag atagcaaaga ccatcacgac aacgctcgcc aggaacggta tacgccatcc
      841 ccacgccagc agttcctctt tactgagaat gaagaacata aaggcccaga ttgccgttgc
      901 gctcaaggtt ccgcagttag ttcccatagc cacaaatgag gagataattc cgcgcttacc
      961 ttttggcgca tattccgcca gcatcgtacc ggcaccggaa atttccgcac ctgcacccaa
     1021 tccctgaata atacgcagcg tcaccagcaa gatgggggca aaaacaccaa tctgtgcata
     1081 ggtcggtaac actccaatta aggtggtaca gatccccatc atggtgatgg taataaagag
     1141 cactttttta cgccctattc tgtcgcccat tttgccgaaa ataaatgctc cgacaatacg
     1201 cgccacataa cctgcaccgt aggttcccat tgccagaatt aacgccattg ccgttgatga
     1261 ttcaggaaaa aatatttcat gaaacactaa cgctgcgccg agcgaatata ac
//
LOCUS       DAZFTN010000346         1300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFTN010000346 DAZFTN010000000
VERSION     DAZFTN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..815)
                     /locus_tag="ACHKV2_005452"
     CDS             complement(<1..815)
                     /locus_tag="ACHKV2_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6943116.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGG"
     gene            792..934
                     /locus_tag="ACHKV2_005453"
                     /pseudo
     CDS             792..934
                     /locus_tag="ACHKV2_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1129..1242)
                     /locus_tag="ACHKV2_005454"
     CDS             complement(1129..1242)
                     /locus_tag="ACHKV2_005454"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6943117.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          322 a          376 c          326 g          276 t
ORIGIN      
        1 ctgccaccat ggcactgcgc gttaaacacg gagaggtcag cacgaaactg tgtcagcatg
       61 gctgtaaaca gcgcaggttg ctgtgcgtgg gtggcggcgc tcatgtcaaa ctcaccctgc
      121 atccagcaga cggccagcag aacgtttttg ggatttttct gcaatgccgc ttttgtgcgg
      181 gaaatcagat cctgatataa cggcttgccc accccccagc gtgccgaatc ctgactggcc
      241 cccgtggact cgctgaatgt cccctccgcg ccctgggtaa atgccgaacc accacgacag
      301 catggtacca gcaggatccc cgcgttattc gggatatacg ggagcagttt tttggcaata
      361 tgtaaaccct ggccgacaca gccgtactgc cctttgctca ggtcagccct cggatgattc
      421 agcgtactca tatcctgcac atcatgcaga cagtggtcag ccggaataat atcgttatat
      481 ctgcaggcag ccccgcccgg cgtcactgta ctgcggcgcg ccagctgttt aatgcgcgga
      541 tccggagcat cgtatgaatc cggcagcgga agcccttcac cgtaagccat ggcattggac
      601 tgcccggcca gtacgatgac gtagtaccaa tccggctcag atgaagggcc gacctgtggc
      661 tctccttcaa tagccaccgc ctgcatcagt gtgtacggcg taatggcaac cggtccgccg
      721 tatggctgcc agccctcttt cagtttgtgt gtcagctttt ccgcaaggtc tgacggcgac
      781 gccgccctga caacatcata atgtttaatc gacatcgaat ttctcccgtg tagaggaaca
      841 gagttaaaaa gccggaagcg gaatcaaatc acaggaagac catctgccag tggctggtcg
      901 taaaaaaagg ccgcgccatg cgcagccgaa aataaaggga taacgatgat agtttgagaa
      961 aaacagaaat aacacttttg tggcaaagca tggtgccggg tgcctcccgg tgaattcagt
     1021 atcagcacct gaatccgcga ttaccccata ttccttcttg ctgattgccc caccgcacag
     1081 ggggattcac catgcagaag tgtttttaat aaacagcaaa caaaaaaatc aagcattatg
     1141 caggctgttt ctttttatca ccggccacag caataccata atgccgcaga ccagcacccc
     1201 atccgccagc accgacatga ttctgctggt gaaatccacc atcaccacca gaaacagcag
     1261 gagtgcagcc acagtcaggc gcagttttac cgtcacaggt
//
LOCUS       DAZFTN010000347         1297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFTN010000347 DAZFTN010000000
VERSION     DAZFTN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1297
                     /gene="nleA"
                     /locus_tag="ACHKV2_005455"
     CDS             <1..>1297
                     /gene="nleA"
                     /locus_tag="ACHKV2_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO6943118.1"
                     /translation="QPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVVNIP
                     DDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNVTQM
                     ALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPLKNA
                     LSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVKTEN
                     GWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLDNP
                     NTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPGLKP
                     DTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGADIRA
                     MEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFD"
BASE COUNT          411 a          275 c          273 g          338 t
ORIGIN      
        1 ttcaaccgac catacaatct ggaatcacct cacaaaacaa tcaacatcat caaacagaac
       61 aaataccctc tacacaaata ccgcaatccg aattacctct aggatgccaa gctggatttg
      121 ttgttaatat tccagatgat atacagcaac atgcaccgga atgcggtgaa acaacagctc
      181 tactgagctt gataaaagat aaaggtctgc tctcagggct agacgaatat atagctcctc
      241 accttgaaga aggatccata ggaaaaaaaa cattggatat gtttggttta ttcaatgtta
      301 cccaaatggc attagagata cctagttccg tttcaggcat ctctggtaaa tatggtgtcc
      361 agctaaacat tgtaaaacca gatattcatc ctacatcagg taattatttt ttacagatat
      421 tccctctgca tgatgaaata ggttttaatt ttaaagacct tcctggcccg ttaaaaaatg
      481 cattaagcaa cagtaatata tcaaccactg cagtgtcgac tattgcatcg actggaacat
      541 cagccactac ttcgacggta accaccgagc caaaagaccc aataccatgg tttggattaa
      601 cagctcaagt ggttcgtaat catggtgtag aacttcctat agtcaaaact gaaaatggat
      661 ggaagcttgt tggagaaaca ccacttactc ctgatgggcc gaaagcaaat tacacggagg
      721 agtgggttat cagaccggga gaagcagatt ttaaatatgg tgcatctcca ttacaggcaa
      781 ctctagggct ggagtttggc gcacatttca agtgggattt agataaccct aatactaaat
      841 atgccgttct taccaatgct gccgcaaatg cgcttggtgc tttaggggga tttgcagtat
      901 ccagatttgc tagtacagat ccaatgttaa gtcctcatat cggtgcaatg gttgggcaag
      961 cagcagggca tgccatacag tataataccc ctggattaaa gccagacact attttatggt
     1021 gggctggtgc gacactgggg gctgccgatt taaacaaggc cgagtttgaa gtagctagat
     1081 tcactgacta tcctcgtata tggtggcacg caagagaagg agctattttc cccaataaag
     1141 cagatattga acatgccaca ggtgctgata tacgcgcaat ggaagaaggt atccctgttg
     1201 gacagcggca tccaaatcca gaggatgtgg taatcgatat cgaaagcaat ggcttaccac
     1261 atcataatcc atcaaatcat gttgatatct ttgatat
//
LOCUS       DAZFTN010000348         1296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFTN010000348 DAZFTN010000000
VERSION     DAZFTN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            174..>1296
                     /gene="espG"
                     /locus_tag="ACHKV2_005456"
     CDS             174..>1296
                     /gene="espG"
                     /locus_tag="ACHKV2_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO6943119.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAAS"
BASE COUNT          410 a          268 c          245 g          373 t
ORIGIN      
        1 atcaattatc catcattaga ttaatatttt cacttatttt tcagaaggta aaaataactt
       61 tctatgcgtt taaaacaccc gctataaata tcttgtatat tattgacact atatgatgct
      121 atatgatact tgttgccaaa ttgttcatta caaaccagat aggagaatct ctcatgataa
      181 atggacttaa taatgactcc gcatctttag ttttagatgc tgcaatgaaa gttaattctg
      241 ggtttaaaaa aagctgggat gagatgtcat gcgctgaaaa gttatttaaa gtacttagtt
      301 ttggtttatg gaatccaacg tacagtcgta gtgaaagaca atcatttcaa gagttgttaa
      361 ccgttttaga gcctgtatat ccacttccca atgaattagg cagagtatct gctcgttttt
      421 cagatggttc atccttaaga atttccgtca ctaacagcga acttgttgaa gccgagattc
      481 gcacagcaaa taatgaaaag attactgtgc tcctggagtc aaacgaacaa aataggttat
      541 tacaatcttt acccatcgat cgccacatgc catacattca ggttcatcgt gccttatctg
      601 agatggacct gactgatact acctcaatgc gcaatctact tggttttacg tcaaaactat
      661 caacaacctt gattcctcat aatgctcaaa cagatccgct ttccgggcct acaccattca
      721 gctctatctt tatggataca tgtcgaggac tcggcaatgc aaagctttca ctcaatggtg
      781 ttgatatacc tgcaaatgca caaaaattgc ttcgcgatgc actaggactt aaagacacac
      841 attcatcacc aacccggaat gttatagatc atggtatttc tcgccatgat gcagagcaaa
      901 tagcaagaga aagcagcggc agtgataaac agaaagctga agttgtggaa tttttatgcc
      961 atccagaagc agcaacggcc atatgctcgg ctttctatca atctttcaat gtgccagcct
     1021 taacgttgac acatgaaagg atctctaaag ccagtgaata caatgcggaa agatcattag
     1081 atacacctaa cgcttgcatt aacatcagta tctctcaatc atcagatgga aacatttatg
     1141 ttaccagcca tactggggtt ctgataatgg cgccagaaga ccgccccaac gagatgggca
     1201 tgttgacgaa caggacttct tatgaagtgc cgcaaggtgt gaaatgtata atcgatgaaa
     1261 tggtaagtgc gctacaacca aggtatgccg catctg
//
LOCUS       DAZFTN010000349         1292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFTN010000349 DAZFTN010000000
VERSION     DAZFTN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1292
                     /locus_tag="ACHKV2_005457"
     rRNA            <1..>1292
                     /locus_tag="ACHKV2_005457"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          324 a          305 c          406 g          257 t
ORIGIN      
        1 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
       61 gatgtgccca gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc
      121 tagctggtct gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg
      181 ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg
      241 tatgaagaag gccttcgggt tgtaaagtac tttcagcggg gaggaaggga gtaaagttaa
      301 tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt gccagcagcc
      361 gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc
      421 ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat ctgatactgg
      481 caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga
      541 tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc tcaggtgcga
      601 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcgac
      661 ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc gaccgcctgg
      721 ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga
      781 gcatgtggtt taattcgatg caacgcgaag aaccttacct ggtcttgaca tccacagaac
      841 tttccagaga tggattggtg ccttcgggaa ctgtgagaca ggtgctgcat ggctgtcgtc
      901 agctcgtgtt gtgaaatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctttgtt
      961 gccagcggtc cggccgggaa ctcaaaggag actgccagtg ataaactgga ggaaggtggg
     1021 gatgacgtca agtcatcatg gcccttacga ccagggctac acacgtgcta caatggcgca
     1081 tacaaagaga agcgacctcg cgagagcaag cggacctcat aaagtgcgtc gtagtccgga
     1141 ttggagtctg caactcgact ccatgaagtc ggaatcgcta gtaatcgtgg atcagaatgc
     1201 cacggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccatgg gagtgagttg
     1261 caaaagaagt aggtagctta accttcggga gg
//
LOCUS       DAZFTN010000350         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFTN010000350 DAZFTN010000000
VERSION     DAZFTN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..156
                     /locus_tag="ACHKV2_005458"
     CDS             <1..156
                     /locus_tag="ACHKV2_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6943120.1"
                     /translation="TIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEACRRRLSTERS
                     GMNYIIK"
     gene            complement(286..426)
                     /locus_tag="ACHKV2_005459"
     CDS             complement(286..426)
                     /locus_tag="ACHKV2_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943121.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(783..1007)
                     /locus_tag="ACHKV2_005460"
     CDS             complement(783..1007)
                     /locus_tag="ACHKV2_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943122.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1031..>1285)
                     /locus_tag="ACHKV2_005461"
     CDS             complement(1031..>1285)
                     /locus_tag="ACHKV2_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6943123.1"
                     /translation="LADARAENETLRADVAAGRKRLRINATCPGTVREATGTSGVGND
                     AAVELSPVAGRNVLGIRDGIISDQAALRMLQEYIRTQCIN"
BASE COUNT          388 a          317 c          270 g          310 t
ORIGIN      
        1 acaatccgca atatccgttt ccccaatcac atgattgaac agatcaatat cgcccttgaa
       61 cataaaggtt ccggtaactt ttcagcgtgg gttattgaag cctgcagaag aaggctgtca
      121 acagagcgtt cgggtatgaa ttacataatt aagtaacatg gtgttcacag aacacacagt
      181 taccggacac atcagttttc cattcgtccc ccggcagtac aggcttcccg tctgacggga
      241 tagcctgaaa aaacacagaa aattatttgt tataattaat ataacttact caaaaaaaag
      301 cgacgagaaa atcagcatca acgagcaata agcgccaata cgtgataaca aatggcagcc
      361 atatttatct gcagtataag caatggacag gataaccaca ccagaaaccg tcagcataaa
      421 atccatttga acttccccgg acaaaatcga ctcatctaaa gatttacagc tctttttatt
      481 atcaatatgt taaaagtaaa ataaacagat gttcaataat acgaatacaa aaacgtgctg
      541 aaaatcaatg aatccatttc tgtgttatca attaatagtg ataaacatcc ggcttcttcc
      601 accatcgcac cggaccagcg accatgaggg gacaacgccg cgctccgtta acgcggtaaa
      661 ccccggtgtg tatcgttttt gattatcccc gcacactcgc gcagaggatt ctcccggtcg
      721 ggctgcggtc tctgttaatg caggaatacg gcgacaatac cgcgcatgaa taataaggtc
      781 gctcaacaca ctggctgtaa tgcagcggat accatgcggc atttagcggc attcatcgta
      841 cactcaacgg ttagctcttc attcgtggca ttcacctgaa aggtcctgga gtgtaattgc
      901 gtacatttac cactgaacga accttcaaca agaacacgac cacgctgcaa aatacggaac
      961 ggaattgttc cctgaaaagg ctttacggtt accaataatt tcttcatgca ttctccgaat
     1021 aacaaaaata ctagttaata cactgagtgc ggatatattc ctgaagcatt ctcaatgctg
     1081 cctggtcgct gatgattccg tctctgatac cgagaacgtt tcgtccagca accggagaga
     1141 gttcgacggc ggcatcattg cccacgccgg aggtgccggt ggcttcacgc acggtaccgg
     1201 ggcaggtggc gttgatgcgc aggcgcttac gaccagcggc aacatcagca cgcagagttt
     1261 cattttcagc tctcgcatcg gctaa
//
LOCUS       DAZFTN010000351         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFTN010000351 DAZFTN010000000
VERSION     DAZFTN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(223..729)
                     /gene="espH"
                     /locus_tag="ACHKV2_005462"
     CDS             complement(223..729)
                     /gene="espH"
                     /locus_tag="ACHKV2_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFO6943124.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(760..>1285)
                     /gene="escQ"
                     /locus_tag="ACHKV2_005463"
     CDS             complement(760..>1285)
                     /gene="escQ"
                     /locus_tag="ACHKV2_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO6943125.1"
                     /translation="NRLNVIILETTVQSLQYLINDNWQLVPHSNALFFDGYIVPGWTD
                     YPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNLSVTDVLAADLLSALS
                     NETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIAYGSIVK
                     INEDFYFTVSIVCD"
BASE COUNT          406 a          269 c          215 g          395 t
ORIGIN      
        1 cagacctaca gattttttac cttgataatt tctgcgctgg atgggatcat ccattccgtt
       61 taacatactc ttcatcacag ggacctctga tttctggccg ctaacgtctc tgaagattca
      121 gcctgtatta tctaaaagca atgtaatatc agttaaaaag cggaaaaact ccagaattat
      181 aactattctg gagttaagtc aggaagaaaa ctcaaatgct aatcataata cgctataaga
      241 ggaagctctt gttgctatag ccctattatc aagacgttcg gtagactgta cacgacgtaa
      301 caaagaagat aatcctcttt cagctaagat cccctccagg ggagtacgag gaaaccaggt
      361 tgaagaaagt ggagttatat gtctctcatt taattttctt tccaaagcct tagtcacacc
      421 gccagaaaac acgctaaata gcgttgagaa acatccattt tccggtgtta ttctttgtac
      481 tttgtacaca taatcatctt ttgggccatc tttaataaag tgaacagcat agtctttgcc
      541 tctaaaagag acaatggtgg aatcccctat ttctggatat acccgatgat ggcgttgttg
      601 cattcgggct ttaagctgaa tttcatctac gatatcttca ttgggtatat gaagccttct
      661 gttgaacctg ttccatgacg catgttcact tgtcagaaat gtcttgaata ccgctcctga
      721 taacgacatg atacatctcc ctatataacg tattcctgat taatcacata ctatgctaac
      781 agtaaaataa aaatcttcgt tgatttttac aatactcccg tatgctatcg tcgtattatt
      841 gcgtattaac tgacaaccac caaaaagatg gtatccatct gaattaataa tgtcatcttt
      901 tttaacatta cgcaacatgt gtatatcaat atggactgta ccgatcgcac aataaatgcg
      961 attcatgaca gtctcattgc taagagcaga taacagatcc gcagccagaa catccgtgac
     1021 agataaatta ttatcactca gatttactgt tgctaaaggg gtattaataa caatccagca
     1081 ccttctctct tgactatcat caacatgata gagtctcaga ctatcaccaa ctctcaactc
     1141 tgtaactgga taatcagtcc acccaggtac gatatagcca tcaaaaaaaa gagcattaga
     1201 atgtggaact aattgccaat tatcattaat cagatattgt aaactttgta ccgttgtttc
     1261 gagtataatt acattaagtc tgttt
//
LOCUS       DAZFTN010000352         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFTN010000352 DAZFTN010000000
VERSION     DAZFTN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..441
                     /locus_tag="ACHKV2_005464"
     CDS             <1..441
                     /locus_tag="ACHKV2_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6943126.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHKV2_005465"
     CDS             597..779
                     /locus_tag="ACHKV2_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943127.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHKV2_005466"
     CDS             792..923
                     /locus_tag="ACHKV2_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943128.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..1224
                     /locus_tag="ACHKV2_005467"
                     /pseudo
     CDS             931..1224
                     /locus_tag="ACHKV2_005467"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1166..>1285
                     /locus_tag="ACHKV2_005468"
     CDS             1166..>1285
                     /locus_tag="ACHKV2_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO6943129.1"
                     /translation="MKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPVP"
BASE COUNT          317 a          282 c          377 g          309 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc cgctggtcgt
     1201 aagcgcctgc gcatcaacgc caactgtcca ggctccttgc gtaaagcccc catcacctcc
     1261 ggcgtggata atgcaaccgg tcccc
//
LOCUS       DAZFTN010000353         1279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFTN010000353 DAZFTN010000000
VERSION     DAZFTN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..168
                     /locus_tag="ACHKV2_005469"
     CDS             <1..168
                     /locus_tag="ACHKV2_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6943130.1"
                     /translation="RQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGG
                     DGKVVVIGRQA"
     gene            complement(359..886)
                     /gene="sodC"
                     /locus_tag="ACHKV2_005470"
     CDS             complement(359..886)
                     /gene="sodC"
                     /locus_tag="ACHKV2_005470"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6943131.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1020..>1279
                     /locus_tag="ACHKV2_005471"
     CDS             1020..>1279
                     /locus_tag="ACHKV2_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943132.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGA"
BASE COUNT          313 a          299 c          370 g          297 t
ORIGIN      
        1 agacagaaca cgtacttttc ctcgctggat aacatgattg cccaggggaa cccgatgccg
       61 gtgccttacg gtgaaatgct ggttggctcc cggcgaatct cccaggacat cagtacccgt
      121 gatgaaggcg gtgacgggaa ggtggtggtt atcgggcggc aggcataaaa gcgaaaaaat
      181 cccgcagtgc tcacggacag gaactgcggg agcgttacga agattgagtg taaggaatta
      241 ttcttatgtc acgacaaaaa cattaactca gagagggagg atgtgccgtt cttttcaggg
      301 agaaaggatt tatcgtcctg aggaataaag gtaaggggcc cgccccttac ctgactgatt
      361 attgaatgat gccgcaggcc attctcgcac caccaccgcc caggggctcc ggatggtcat
      421 gatggttatc accgccagca tgaagcatga gagaacgccc tttaatctct tttaatgagt
      481 tcagtctcgg ggccaggacc gggtagttcg cttttccgtc atgcgtcacg aacagcgcag
      541 ggaggtcgcc caggtgtcca tccggagacc aggggccaag atgtttgccg gtgtttttcg
      601 ggtcaaagtg accgccagcc gataatgctg cgaccggttt tccgtctttc agtgccgggg
      661 cgcaatttcc tttttcgtgc acatgaaaac catgaatgcc ttcagacaga gagtgaaggg
      721 ctggtgtgaa cagcagaccg tagggggtct cctgaatggt tatttttcca atgctgactt
      781 cttttccgtc agcactgaca aggttcattg ggacttcctg ttctgctgcg tatccgcatg
      841 atgctgctgt cagcatggca atggcagcaa tgattttaca tttcataaaa ccctcattaa
      901 ttccgttaac agactgagct tgctggttac agggtaacaa acagcgttct gatgatatcg
      961 cgcaatagct gtgcaatatc ctatcactgc gattaataat accaattgag aggaacatta
     1021 tgggtaaagg tggcggcagg gcgcacacgc cggttgaggc aaaggacaat cttaagtcca
     1081 cgcagatgat gagcgtgatt gatgccattg gtgaagggcc gattgaaggt ccggtgaagg
     1141 ggctgcagag tatcctggtg aacaaaaccc cgctgacgga cacggacggt aatcctgtga
     1201 tacatggtgt gacagcggtc tggcgcgccg gggagcagga gcagacacca cctgaaggct
     1261 ttgagtcttc cggagctga
//
LOCUS       DAZFTN010000354         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFTN010000354 DAZFTN010000000
VERSION     DAZFTN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            185..367
                     /locus_tag="ACHKV2_005472"
     CDS             185..367
                     /locus_tag="ACHKV2_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6943133.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            403..648
                     /locus_tag="ACHKV2_005473"
     CDS             403..648
                     /locus_tag="ACHKV2_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6943134.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(687..1153)
                     /locus_tag="ACHKV2_005474"
                     /pseudo
     CDS             complement(687..1153)
                     /locus_tag="ACHKV2_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          375 a          261 c          290 g          339 t
ORIGIN      
        1 gcattgtggt ggaagtgaaa aaagcggtaa cagaataaaa aaatcccgcc ggtcatgata
       61 tggctggcgg gagctaccta acccataaag gaaaataaaa atactcccga aggagtggtt
      121 atcagtcgct tcgatgtttt tacccgataa cgaattatgt agtcaatatc aaaggtaagg
      181 ttatatgaca ttcgttcata caatgctgct ttatttctgc gctgtggtca gtgcgttata
      241 tctggtgagc ggcggttata aggtcatcag aaattatatt cgccgtaaaa ttgatgatgc
      301 ggcagctgaa aaactcagta aaacagcaca ggcaccgtca tccccaaacg acccgacccc
      361 gctctgataa cggggcaata tacctgcaaa aggagaatat tcatgccaga gattaaaggc
      421 acggttactg aagagcttgt caaacaggca ctttattctg aagaagtgaa ccgcgtgctg
      481 aaggcgcagg ttcgcaagga ttttgaggca caaatcgacg catatgttga tgaagtgctg
      541 gcccgaatgg ttggccgttc tccggctgaa aacagcacgg aaaatgatcc tcaacccgta
      601 gagcaaccag agccggttca gcccggaact gacggtacta tgatgtaaca ataccgaccg
      661 gcaggtttcc ctgccgggtg agtttgttaa tgcacgtttt gtgtattttc cctcctggga
      721 gaacaggcag gacatatcag catcctgaaa caggaagcat ttttgggttt gtatgtcttg
      781 ccattttgag atcgagaacg gcgatagatg agctgtaagg gagtcttaca ggcagggcaa
      841 acaggctctt tcatccatga taaagatctc ctttttatgc atctctgctt tacaggagac
      901 tggggaatat cataggatac gccacgagct cgaagtaaag cattgatgca aaattgtcta
      961 aatcggccca gaatagttcg cgctatctgg gtcgctcttt atgtatgtag tatcccattg
     1021 atttgaaaca tactacatat acgcgcgatc ctaccttcac tatataaaaa gtcaataaaa
     1081 tgatcatttt atgatcgttt tcaccaccta actcttcgtt ttctttaagt aaataatcac
     1141 ctacctgaat cattgttgat gacttttcaa tctatttgtt gtatcaatta aacaaatcga
     1201 atcgatcgtt tttgtcgatc gattgcatga gaaaggcatc aacaacaaag caggaggtta
     1261 tgcgg
//
LOCUS       DAZFTN010000355         1264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFTN010000355 DAZFTN010000000
VERSION     DAZFTN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..184
                     /locus_tag="ACHKV2_005475"
     CDS             <1..184
                     /locus_tag="ACHKV2_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO6943135.1"
                     /translation="LSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLALISVATCWLL
                     WKYRAILTNLVRLKIK"
     gene            200..781
                     /locus_tag="ACHKV2_005476"
     CDS             200..781
                     /locus_tag="ACHKV2_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO6943136.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYNPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILS
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            774..983
                     /locus_tag="ACHKV2_005477"
     CDS             774..983
                     /locus_tag="ACHKV2_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943137.1"
                     /translation="MIKRKISDGLKEIVSLKEKILLETTAKIQSIEEKREEKFIQGYY
                     DGYTKGIIDVMDNFIPLISLAMLRT"
     gene            1000..>1264
                     /locus_tag="ACHKV2_005478"
     CDS             1000..>1264
                     /locus_tag="ACHKV2_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943138.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWVTKLPSISGPVNLHIPTS
                     FKDKSLEVESYFVDKSIWNVHIAYHDDKRFVFFTDQFIAEFSPQ"
BASE COUNT          431 a          203 c          218 g          412 t
ORIGIN      
        1 attgtcaaaa cgaagagaca ttattgagca agcaccaaca tatgaaatta gcgaacctgt
       61 ttttgcttat gataaggcta tgccggtttc tatcttactt gcattaatat ccgtagcgac
      121 ttgctggctg ttatggaagt atagggccat tctaactaat ttagtcaggt tgaaaattaa
      181 gtagacatat gagaacgtta tgaatcttgc cctaagaaag attatatatg ctccaattag
      241 ttatattcat cctcaacgag taagcttgaa taatacacct attaacaatc cggtgctgag
      301 atctattacc aatgaaatga ttctcctgca gtataatctt tcagttgaac attttaatct
      361 gaatagttca cttatttact acataaacaa ttggaattta cttccattga tctgtttact
      421 cagtggttgt cacttttatc gagaaaggtt tgcagaacgc gggttttttt ataaggttcc
      481 tgatgtacta agggattatt tatcagctat accgctggaa ataaatgaaa aggcacgata
      541 caaccccggc atcgctaact atcacaatat catcacatgt ggtttttcaa cgttgttacc
      601 ctatataagg caacaaccat tggcaatgca gcaacgattt aatttattat ttcctgattt
      661 tgttgaccac atactatcgc cgttaccact tgcatcaaca cttttagaga gaattacatt
      721 ttatgcgaaa aaaaatagag atgagcttga taaaatctcc tgcaaatggt gttgtgatta
      781 aaagaaaaat ttctgatgga ttgaaagaaa tagtttctct taaagaaaaa atccttcttg
      841 aaaccaccgc aaaaattcag agtattgagg aaaagagaga agaaaaattt attcaaggtt
      901 attatgatgg ctatacaaaa ggcattattg atgtgatgga taattttata cccttgataa
      961 gcttagctat gctcagaact tgaaaaaaaa agaataaaca tgataaacga tctgaagagt
     1021 atattgttaa aatcttcaga agaagttgat gtttttatca aaatttttga aagttgggta
     1081 acaaaattac catcaatatc aggtcctgta aatctccata tacctaccag tttcaaagat
     1141 aagtctcttg aagtagagtc atattttgtc gacaagtcaa tatggaatgt ccatattgca
     1201 tatcatgatg ataagcgttt tgtgtttttt accgatcaat ttattgctga atttagtccg
     1261 cagg
//
LOCUS       DAZFTN010000356         1261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFTN010000356 DAZFTN010000000
VERSION     DAZFTN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..88)
                     /locus_tag="ACHKV2_005479"
     CDS             complement(<1..88)
                     /locus_tag="ACHKV2_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943139.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAV"
     gene            complement(216..632)
                     /locus_tag="ACHKV2_005480"
                     /pseudo
     CDS             complement(216..632)
                     /locus_tag="ACHKV2_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            620..1261
                     /locus_tag="ACHKV2_005481"
                     /pseudo
     CDS             620..1261
                     /locus_tag="ACHKV2_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
BASE COUNT          325 a          308 c          300 g          328 t
ORIGIN      
        1 aaaccgcgat atcgttaata tcgatgtcgt ctttatttat cctgtcataa taaaaatgct
       61 tcccggaaaa agttttaata aatgacattt tgttctccac gtatatgcgc tgcaccgcgc
      121 tgaattctgg taaaaagaat ccctcaccat ccggcgatta ttgagtaaat tacgtttcca
      181 taaatgcccc ctcaggggca tttgcagtaa tgaaatcagg cggtgaaagt accaataaag
      241 gtttctactt tgctgtcctt gaatttctca acaagcagat cacgaaattc gttagccatt
      301 tcttcctgca ccgcctccag ctgaataatg cgcagaacca gtaccggacg atcgccagtg
      361 ataatgctga ggcgtaattt aaacggacgt tctttcagac cttcaaacgg aatgcattta
      421 aattcaaatg ccactggcat aatgtctttg gtcttcgctt cgacagactc catcagggag
      481 cgtttgccgc tgaagtcatt atcttcaaaa tcagcggtct ggtttgcttc aatcgtgatt
      541 ttacggaccg ccgcagccgc ttttgttgcc tgaatggtgt caccattagc atcaaagccc
      601 acaaggtagt cggcccagtc ttcaatccat tctggttgcc gccaatcgta tcgcgtttat
      661 tgacccggca aacgggaatg aaacgccgat gtttgtggcg cagggcaacc agatattcat
      721 gaacgacgtg ttcctgaagc gcctgacggc ccccaccatt accagtggtg gaaatccacc
      781 ggcattttcc ctgacaccgg atggaaagct gactgctaaa aatgcggata tcagtggtaa
      841 cgtgaatgcg aactccggga cgctcaacaa cgtcacgatt aacgagaact gtcgggttct
      901 gggaaaactg tccgcgaacc agattgaagg cgatctcgtt aaaacagtgg gcaaagcttt
      961 cccccgggac tcccgtgcac cggaacggtg gccatcaggg accattaccg tcagggttta
     1021 tgacgatcag ccgtttgacc ggcagattgt tattccggcg gtggcattca gtggcgctaa
     1081 gcatgagaga gagcatactg atatttactc ctcatgccgt ctgatagtgc ggaaaaacgg
     1141 tgctgaaatt tataaccgta ccgcgctgga taatacgctg atttacagtg gcgttattga
     1201 tatgcctgcc ggtcacggtc acatgacgct ggagttttcg gtgtcagcat ggctggtaaa
     1261 t
//
LOCUS       DAZFTN010000357         1259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFTN010000357 DAZFTN010000000
VERSION     DAZFTN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(246..524)
                     /locus_tag="ACHKV2_005482"
     CDS             complement(246..524)
                     /locus_tag="ACHKV2_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO6943140.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            952..1098
                     /locus_tag="ACHKV2_005483"
     CDS             952..1098
                     /locus_tag="ACHKV2_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943141.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          384 a          223 c          244 g          408 t
ORIGIN      
        1 ttttcgtgtc tttaagtgat accagatggt attgcgctaa atgtaccaat gggtatacca
       61 agagcactaa aaacaaaggg gcaaccactt tgtaacccct tgattatttg cagaagcgca
      121 gaaaatcgaa ctctggaacc ctttcggggc atcctttttc aagaatgaag aaaaaaataa
      181 ctaaaaaatc aagttgttag gaagttattc atggctagtg taccaactct tttctgggct
      241 aatagctatg atttgtttgt attatttgtc tctgtctcta ttatttttga catccatgcg
      301 acttctgata ttgataacct cagttgtgga gttctgtctg cgcttccggg aggacgcata
      361 tcaaaactac aggatcttct gttaaacact gtaaagccat ctacattgca tgttgttgag
      421 tctaagatac aatttttagt agatgattta tctgctatag gcctagatat ttcaggttga
      481 tgccaagtgt gtcctgaaaa aatacttttt attgaaaatg gcatttttat cctcacatta
      541 tgtctttctc ttatagtgca atttaatgta tttagaaaca ttaaactatg aatctaaaaa
      601 ggctacgcag agtactgtta cactttaaca aatcatattt accactctgg aaatgggatg
      661 gctggttcat aaaataatga gtctttttca acggttgtta cattggtgta ctgatgatta
      721 tgccttgtcg ctaagagcta ccataaccgt acagcgtgat tcatccatgg cgaataaatc
      781 gactgataaa tccccctgag tttcagctct tgctgttgga ccttgcccgt ttcgctatgg
      841 aacgcgtacc agctagagaa tattattcgt tctcctccaa tgttacgcaa gttttgtaaa
      901 cggtaaccaa tcgtgattag tcctgcatgt aacggacatc aagctgtctg gttgattttt
      961 attgccagta gttcgagaga gcagacaact gcgtccgatg gaaaaatggg cacgacaggg
     1021 tattcaggca acctcgctga ggaggcttat ggtacagatt gtaatgtgaa gtataaaacg
     1081 ttaaaaattc cattttagaa actaaatagc tgtaatataa gttcgatcta atctactatt
     1141 ttgacagaca gattgtatca agaattaggg agtatgaaat agatggcaat aactagtaaa
     1201 attaattttg caacatttaa atcgaaccat acagtcaaat tctagtgcat atattttgt
//
LOCUS       DAZFTN010000358         1250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFTN010000358 DAZFTN010000000
VERSION     DAZFTN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1250
                     /locus_tag="ACHKV2_005484"
     CDS             <1..>1250
                     /locus_tag="ACHKV2_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6943142.1"
                     /translation="LIDEQASRVKSLQEKAQSIQDVLAGLEDRRVALIRQQAAEQNKV
                     YQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTEREL
                     ALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGGK
                     SETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTALGELKTLTEMQKQELLRNAT
                     LIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRDSFRQ
                     KDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQAVFYAESDVQRADWQKGLQEG
                     FSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLMN
                     AAIVNGIKTAANGMSGAGGFLGSIG"
BASE COUNT          340 a          276 c          409 g          225 t
ORIGIN      
        1 ctgattgatg aacaggccag tcgggtgaaa tccctgcagg aaaaagcgca gtccattcag
       61 gatgtgcttg ccgggctgga agaccgtcgt gtggcgttaa ttcgtcagca ggcggcagag
      121 cagaataagg tgtaccagtc catgctggtt atgaacggtc agcatacgga attcaaccgt
      181 ctgctggggc tggggaatga actgcttcag cagcgtcagg gactggtgaa tgtaccgtta
      241 cggctgccgc aggccaccct ggatgataaa cagcagagtg ccctgacaaa aacagagcgt
      301 gagctggccc tgtccagact gaaaggggaa gaaaaagagc gtgtccgact ggggtatgcg
      361 gcggatgacc tcggttttgt gggtgatccg tatcaggagg cgagacaacg ttatatcagt
      421 aatgccctgg aagcctggcg caataacgag gcgaataaac ccaaatcccg gggtggaaaa
      481 tcagagacgg aaaaagcgga agacagtttt tcccggctgc tgaagcagca gaaagagcaa
      541 ctggcactgg tggggcagaa tacagagctg gcgaagctga aataccagac agcgctgggt
      601 gaactgaaaa ccctgacgga gatgcagaag caggaactgc tgcgtaacgc gaccctgatt
      661 gaccagcaaa aaatccggga acagttgcga tcccgggaag agacactgaa gaatgagaat
      721 gcggctgcgc gtgcgtcgaa tgatgctgaa ctgctggggt acgggcaggg ggagcgagcc
      781 agagaacgca tgcgggagtt gcagcagatc cgcgacagct tccgccagaa ggatgcggac
      841 cttcagtctc agtatcagac cggggatatc agtgaggatt tttacagaca ggctctggca
      901 cagaacgcgc agtatctgag cgaacgcctt aaggaccagg cagtctttta tgccgaatcg
      961 gatgtgcagc gtgcggactg gcagaaaggg ctgcaggagg gattcagtaa ctgggtggat
     1021 aatgcgtccg attacgcctc acaggcagca cagctggcga cggagggtat ctcagggatg
     1081 gtgaataaca tcacggagat gctgaacgga aataaagtgg aatggcgcag ctgggcctca
     1141 tcagtgctgc aggaaatatc aaaagttctt atgaatgccg cgattgtcaa cggaattaag
     1201 acggcggcaa acggtatgtc cggtgcggga ggatttctcg gcagcattgg
//
LOCUS       DAZFTN010000359         1250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFTN010000359 DAZFTN010000000
VERSION     DAZFTN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..80
                     /locus_tag="ACHKV2_005485"
     CDS             <1..80
                     /locus_tag="ACHKV2_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943143.1"
                     /translation="RGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(116..>1250)
                     /locus_tag="ACHKV2_005486"
     CDS             complement(116..>1250)
                     /locus_tag="ACHKV2_005486"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO6943144.1"
                     /translation="KIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPG
                     YSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVV
                     HNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADI
                     EIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAA
                     HKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADL
                     VVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSD
                     ILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          392 a          248 c          212 g          398 t
ORIGIN      
        1 tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc ggcttattat
       61 tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata atgcggcctt tttatttatt
      121 caaaccaatt atgaataacc tcttcgaatc tttgagttac gccatcccaa ctgtagtgat
      181 caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa ttcaggattc gctaacgttt
      241 ttaaaatatc gctgcttatg gagtctgcag tcataggttc ttttaaatgg aatcccgtag
      301 tattttcttt gacaaattcc gtcatccccc cacgcgtact cactaaaaca ggcttaccag
      361 cacccatagc ttcaattgcc accatgcaaa atggttcctg gaactgggaa ggaatgacga
      421 ctaaatctgc cagtgggtaa taacaatgca tcttttcagg cggtatgctt cctaacataa
      481 tgcaacgatc cttcaaacgt tttgcaattt ccctgacatt gcgttgatat gcacctttgt
      541 cacttttact catttctgta taatcaccaa tcacaactaa ttttaaattt ttgtgagcag
      601 cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg acgatacgcc
      661 cagcataaaa aataattttc tcttcagggg agatattaag ttcgcttcta gtgatcggct
      721 gaaaattaga ttgataggtt tctaaatcaa ttccatttgg tacaatctca atatctgcat
      781 ttgctaaata agattggtaa tactttttca agtataaact aggcactatc attttaacat
      841 tttgctcaag caattttggt tcaaatgcat tatgcatatg tataaccact cttgcctgag
      901 gagctcgctt acgaatttga gtgtatagct tcatgctatt atgaactaca ataacacttt
      961 ctttcgttat aggaaaatca tgtgcaatat tcaatattct ctgtgaatat ggaagaggat
     1021 caagtcttgt ccatttttga aataaccgct tatatatgcg actgaatcca accctatgaa
     1081 tactacaatt atcattaaca aaggtgtaat ttgagtatcc tggatttttg atacaaacaa
     1141 tacgatttgg aaaattggta cgctgagcaa cctgatacat ccacgtttca acagcagccg
     1201 ctccgcgagg aggaattgaa aaaataggag taaccgtaaa tattatttta
//
LOCUS       DAZFTN010000360         1236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFTN010000360 DAZFTN010000000
VERSION     DAZFTN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..490
                     /locus_tag="ACHKV2_005487"
     CDS             14..490
                     /locus_tag="ACHKV2_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6943145.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            617..>1236
                     /locus_tag="ACHKV2_005488"
     CDS             617..>1236
                     /locus_tag="ACHKV2_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO6943146.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQM"
BASE COUNT          291 a          307 c          394 g          244 t
ORIGIN      
        1 caggaaaaat aaaatgacca cacgtaagaa aaaaacggca gtttccgagg cagccgtgat
       61 ggaggcaatc cgggaagcac ttgagggggc tgatccacgc actgccgggc tgacagagca
      121 actggcaaag ggatatgtgg atctgcttga tggtctgccg tttggtgaga cccgtgaata
      181 tcgtgtcacg ttccgggaac tgacagcgaa agacagtatt gacgcagaag cagaagccga
      241 gcgcgtggtg gagacaaaca atggcccgat gctgatagcg tctccgtcgt tacgcggtgt
      301 tgcactgctg cgccgtcaga ttgccgcagt gggagacatt gaaggtccgt tgtcaccccg
      361 tcagattgga cagttaagcg agcgcgatct ctcccgcctg atggcagcgg tcagcctgct
      421 ggataccgcg cttgccggaa agctggcagc tgaccggggg cgatcaggcg cagtgtcggg
      481 atcagattga agaagcggca attatcctgg ggatggtgac aaaaagcggt ccggaatggg
      541 cgcttaacct ccccctgtcg caactttacc ggcactgccg acagacagaa aaaatcatca
      601 gaacgaagca gtaagtatgg cccgaaatct cagagcatct ctgatagttg atttgctcgg
      661 caatatctca gccaaatccc gccagtggtc acaggagctg ggggcattct cacgctccgg
      721 ccaggcgggg ctgggtggcc tgggaaatgc tgcccgccgt gccgggcagg aaacagatgt
      781 actcggaagc aggatgcagc gcacgctggc cggggtgcgg ggcagtatcc gccatgtcac
      841 ttctgatttt gaccgtcttc agggcagtat taccggcacc atcgggcgga tcagcaatct
      901 ttacggaatg ctggccggtg gtgctgctgt atatggcttt aaccggggat ttattcgccc
      961 ggcagcggag atggaaacct atatgattcg cctgaactcc ctttacaaag gggatcgggc
     1021 gaaaactgat gatgtccgca ggtgggcaat acagaatgcg aaggaaacca cctgggggct
     1081 ggctggcgtc atgcaggaat atacgtccag tctgggcttt ggtatgagtg acagggaggc
     1141 ccgaaatttt gtcaccatgc ttcaggatca gggtgccgtg ggcggctggt cactgtccga
     1201 tgcacagggg gcatccctgc aactgaaaca gatgta
//
LOCUS       DAZFTN010000361         1232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFTN010000361 DAZFTN010000000
VERSION     DAZFTN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1006..1194
                     /locus_tag="ACHKV2_005489"
                     /pseudo
     CDS             1006..1194
                     /locus_tag="ACHKV2_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          375 a          224 c          281 g          352 t
ORIGIN      
        1 tctgactcgt gtcagtcggt ctctggttgt atccacggac cgcactcttg acataagagt
       61 gcggtccgtg gaaaacggag ttgaaaaaat ggtgtctgaa agaaggtacc atcattgtga
      121 tgacaaatac tttggcgtgg ataatgtaat taattgctga aattgaccgt atatgcggta
      181 agactcatat cagtatcagg tccggttaat tgctgatact aatataaact ttttacaaaa
      241 ggatgaatgc agaaggttac tctaatggcc agagtgatga caatgatggt cagacgttga
      301 cctgcagaaa ctgcatttta attggtctat aaagtctgca agaggaatca gggtcagatc
      361 agtgagtctt atcgcggccc gaagctatga ctgaaagaag gctggataca gaattgcaac
      421 aatttggcaa tgtcagaaca cagaagcttt gcaagcaggt gaaaggttat gtctagcaaa
      481 aggagtatat cagaatatat ctactgatgt acaaaacaca acaatagctt catgatatat
      541 aaatacgtga tgacgagtgg tactccgcat ggttatttaa cttctaagca ccactaatga
      601 tcctgagcaa cttcgtattt gggccattac catggagtag aaagctgtgt ctgagaatgc
      661 agaatttgag aaaagaattc gctttcttgg agagattctg aaaatggcac gctaacaacg
      721 ttttgttaaa aaatgtgaat tacttgccgg ggctgcattt tctattcgag gagaatgttg
      781 acgtagatat tacagaaatc tcagcgcatc aggataaact gctaccttag gtcaaccacc
      841 aaaagaaaat acaatacgtc cagtttgtat gcctttaata acacatcttc taaatgttga
      901 agaattatcc cgcctgctga agaacgttgc tctgagtgta ataaaccttt ttattcttga
      961 tgatgtcagc gagaagatgg aatatatccc cgactgggtc gtggttaccc ggttaaacat
     1021 ctggtggtgc taataaatct ggcaagtcac attctgttga atgctgtgac agtgccttac
     1081 cgagagagcg aaattgtggt gttctagtgc atagtcgcca ccattccgga taattctatt
     1141 acgttgtttt acaagttctt ttacagcgca gatctgtcgc tgacgttgaa tcaatagcct
     1201 ggatttcaga acacaatgcc cgtttgctgg tt
//
LOCUS       DAZFTN010000362         1226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFTN010000362 DAZFTN010000000
VERSION     DAZFTN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..696
                     /locus_tag="ACHKV2_005490"
     CDS             64..696
                     /locus_tag="ACHKV2_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6943147.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(715..1218)
                     /locus_tag="ACHKV2_005491"
     CDS             complement(715..1218)
                     /locus_tag="ACHKV2_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6943148.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
BASE COUNT          395 a          196 c          233 g          402 t
ORIGIN      
        1 aataataatg aaagggatga ttataaatga atgcgttaaa gcatttacac tcaggatgaa
       61 tatatgggaa aaattaaaat tgtagtttca gatcagcagc cgtttatgat tgatgggata
      121 attggattcc tcggacatta tcccgattta tatgaggttg ttgggggcta taaagatctg
      181 aagaaagcta tagcggagtg taacaaatct gcagcccaaa tatttatttt aggagaattt
      241 gcaggtggaa tgatgggggc cgaactcgta aaatgggtta aatcgcataa aatagatgct
      301 catattatta catttgtagc aaaaatgccg tatattgatt caataaaatt gcttgaagca
      361 ggtgcgaaag gatgcgtatg gaaaaccagt cacccggcga aacttaatcg cgctattgat
      421 tcgattagta acggctacac ttattttgat agtgtacata tggattgtga gaaaatatct
      481 tccagatatt cctctgataa tcaacttaca aatcgtgagt ctgaaatatt acaactcata
      541 gctgatggaa aaacaaacaa agaaatcgct aatttcttgc agttaagcag gaaaacagtc
      601 gaaactcata gactcaatat catgaagaaa ttagatgttc acagtggtat cgagttgatc
      661 aaaacagctt tacgtatggg tgtgtgcact atataaaaaa aagaggcttt aggctcattt
      721 tgctaaagcc ttacgattgt ctagttcatt aagcctggta tatacaatat atattttttt
      781 ggcatattgc agtcgctttt tttggttatc cattgcaaaa cccgcattat aagtccccag
      841 gcattgccac gtcattccac aacgggaaaa atgcttatat aaaatctcgg ttcctatttt
      901 aatgttcagg cagggattat ctaacaaatc tcgttcatct tttattattc cacgtttttt
      961 taacatggga atgtgcatct ggtttatctg cattaaacca taatcaaaac tctttatatt
     1021 gtttttctta tctctgttct tccctatgct atcctttttt agattgcttt caacaagagc
     1081 gatagcttta atcaaatttg gttcgattct gaacattgtt cctgcttcat taaagcaagc
     1141 gctactggcg aaagacattt ttgtaattaa tatagaaagc gtgaagatgg ttaagaattt
     1201 aacttttggt gagcgcatat atcttt
//
LOCUS       DAZFTN010000363         1221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFTN010000363 DAZFTN010000000
VERSION     DAZFTN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..294
                     /locus_tag="ACHKV2_005492"
     CDS             <1..294
                     /locus_tag="ACHKV2_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6943149.1"
                     /translation="GYMGTSATMTSGERSGAVIRGVFDDPENISYAGQGVRVEGSSPS
                     LFVRTDDVRQLRRGDTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            309..842
                     /locus_tag="ACHKV2_005493"
     CDS             309..842
                     /locus_tag="ACHKV2_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943150.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            839..>1221
                     /locus_tag="ACHKV2_005494"
     CDS             839..>1221
                     /locus_tag="ACHKV2_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6943151.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          290 a          285 c          373 g          273 t
ORIGIN      
        1 gggtacatgg gaacgtcagc caccatgaca tccggtgagc ggtccggcgc agtaatacgt
       61 ggtgtttttg atgaccctga aaatatcagc tatgccggac agggcgtgcg cgttgaaggc
      121 tccagcccgt ccctgtttgt ccggactgat gatgtgcggc aactgcggcg tggtgacacg
      181 ctgaccatta acggcgagat gttctgggtg gaccgtgttt ctccggatga cggagggagt
      241 tgttatctct ggctcaaccg tgggcaacca ccggcagtta accggcgacg ataaacgcag
      301 ggtgaattat ggcgataaaa gggcttgatc aggcgattga aaatctgagc cgggttcgta
      361 aaaacgccat tccggcggct tcagcaatgg ccattaaccg cgtggccaca acggcgatta
      421 atcagtcttc atcacaggtt gcccgggaga caaaggtacg ccggaaactg gtaaaggaac
      481 ggtccagact gaaacgggcc acggtcagaa atccgaatgc cagaattatc gttaaacgcg
      541 gtgatctccc tgtgattaag ctggggatca ggatgctggg ccgtcgtccg gacagcatac
      601 ttaaagctgg tcagcatcgg tatcagcggg catttatcca gcgattaaaa aatggtcgct
      661 ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg gtgaaaatcc
      721 cgatggcggc cccactgaaa caggcgtttg atgagaatat tgaccgtatc cggcgtgaac
      781 gtctgcccgg agaactggca tacgcgctga aacaacaact taggattgcg ataaaacgat
      841 gaaacatact gatatccgtg cggcagtgct ggatgcactg gagctgcatg aacacggggc
      901 gacgctgttt gatggtcgcc ccgttgtttt tgacgaagag gattttccgg ccgtcgcggt
      961 ttatctgacg gatgcagagt ataccggtga agagctggat gcagatacct ggcgggccac
     1021 actgcatatt gaggtgtttt taccggcaca ggtaccggat tcggagcttg atcagtggat
     1081 ggaaagccgg atttatccgg cggtgactgc gatcccggca ctggcagacc tgattacgac
     1141 gatggttacg cagggctatg agtatcgtcg tgatgacgat atggcgttat ggagttctgc
     1201 ggatctgact tattccatta c
//
LOCUS       DAZFTN010000364         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFTN010000364 DAZFTN010000000
VERSION     DAZFTN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..139)
                     /locus_tag="ACHKV2_005495"
     CDS             complement(<1..139)
                     /locus_tag="ACHKV2_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6943152.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQ"
     gene            complement(364..918)
                     /locus_tag="ACHKV2_005496"
     CDS             complement(364..918)
                     /locus_tag="ACHKV2_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6943153.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(981..>1220)
                     /locus_tag="ACHKV2_005497"
     CDS             complement(981..>1220)
                     /locus_tag="ACHKV2_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6943154.1"
                     /translation="TYFVSKAGERYRRDVALIVRQQRLKLNLSGRLAIKIIAEPPDKR
                     RRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRG"
BASE COUNT          297 a          339 c          234 g          350 t
ORIGIN      
        1 gctgggtcag tattaactgg cagcgttcgc gtgaaaggta agtattctgc gcaatctccc
       61 cgactgtcgc cggttcggta acgcttaatt cattaaacac cactctggcg gtttctgtca
      121 tatcctgctg ttttagcatg tctttttccc ttttccggtt aacgtgacat accaataact
      181 cttgtcgaaa aagccagcaa gttgaaagac cggtattagc aaccaccagc gcgtttaacg
      241 ccccgtgccg tttttcagtc ataaaaaaac ccgcaaaaag cgggctcttt caaatgtcca
      301 tgtctgctat tcgcctcgcg gtacagcttt gcgaagctta ccggaattga agcagttttt
      361 acgtcaaaaa gcaataactt ttttctctat accaaaagcc ataaccattg gtttgtacaa
      421 aataaattct gccaccttta gccaatgctc aatgcgtctt tcacaggttc ttaaactcca
      481 ttccggatgt gcatcattca gcaattcagc cattttgcgc ttagtcatcc cccgccccac
      541 ataacgctga ctcagaacat tgagcagccc gggataacct gccagtactt caccaataac
      601 cctatcgatt attaacgcct ctgaatcggt acaatgtgcc agccagcttt tttgattgcc
      661 gttgatcata tcccgcaaaa aagcctcaag ttcaggtttg tccagacccg cttttttcat
      721 cctccggagc gcctcgttaa ttgccgtttt tgtcagcttt ttagaggtca gtaattggtt
      781 gaacatattt cccgtcttac cgtcgccaat atacgaccaa cgcccccaca tacgcagttt
      841 cccctggatc cagacacttt ccagcgtttt caggcgtaaa tgctcaccgc ttttgcctgt
      901 aatttccggg tatatcatat ttatgctcac tcactttcaa ttttgtaaat cttcacgccc
      961 agccgcccac cagaaacgag ctagccgcgc acaatattga tttcatcaaa ctgctcgtcg
     1021 tctatgagaa gtccggcatg cgtcagcgca tccagtggtg ctttcaggat attgtccagg
     1081 tcacgacggc gcttatccgg tggctctgca ataatcttta tcgccagcct tccggacagg
     1141 tttaatttca gccgctgctg gcgaacaata agtgccacat cccggcgata acgctcaccg
     1201 gcttttgata caaaatatgt
//
LOCUS       DAZFTN010000365         1208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFTN010000365 DAZFTN010000000
VERSION     DAZFTN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..>1208
                     /locus_tag="ACHKV2_005498"
     CDS             168..>1208
                     /locus_tag="ACHKV2_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO6943155.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPA"
BASE COUNT          399 a          220 c          224 g          365 t
ORIGIN      
        1 cccctcgttg acgtcgcggt ttacccacct cattaccagg ttgatgacct gatggggaaa
       61 tgacgtaaaa agacaaataa ctctataaat gagcgaaatt tgatagttga tttttaaata
      121 gtaaccttat tattactaca actttcatat ggatgagggg aaagaaaatg gttgataacg
      181 taactgttag cagagtatgt atacaatctc cttctttcgt gcctgatttg gatggagaaa
      241 aaaataaatc tcaattattc gttgacgata tagttgcgta tcttaaaagt ccttcagttt
      301 attcacttga aaaagagggg ccgttaaatc attttgttaa tcattgttct gaagttgagt
      361 taggtttcta tagcgatggt gcatattcaa ttcttgtctc cagatctaag caacaacctg
      421 aaggtatgat tttaaccgta agcgatgcag acgcaatcaa tatagtacat atttccgtat
      481 ctccagtgct tataaaattc ctggatgata tttttacttg ccttcatacg taccctgatg
      541 atgagagttt tacaaaagag cagataaaag ctaatagcaa atatgatatt gtagattata
      601 attgcctgtt gcattttact ggaaaaccaa aaagtttaat agaatgtaga cattttgctc
      661 tgcaatactg tatagattca atgaatgagc atacagggaa agttccatta aaggcttact
      721 attcatctcc ggaagatata caaaaacata ttcctttcga gcttgagcag caatttaaca
      781 atctacaaaa aaatccacca cccggtacat gcgtcgttgc cagtgataag tttggggagg
      841 cattatctgt cttttttcac agaatggaaa aagagaagtt aacgcatatg acggcaatcg
      901 ttcaatctca aacacacgct atggccgtcc gcttgaggat caaaaaaacg cctgctggtg
      961 aaacagaata tgttgtatcc ttttatgatc ctaatgcaac caatactgca gtacgctata
     1021 aagcaaacaa ctgtgattct tttgggtcat tgcaatcgtt tataaatatt caacaggcaa
     1081 aacaaaaatg ggtaataaca gatatttgct ccgagtgtgt aggaataacc ccttatctcc
     1141 ctcgggaaca agcccattta ttaagtggta ttgaaaatga gttgcaacct ccattatcac
     1201 caccagca
//
LOCUS       DAZFTN010000366         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFTN010000366 DAZFTN010000000
VERSION     DAZFTN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..555
                     /locus_tag="ACHKV2_005499"
     CDS             <1..555
                     /locus_tag="ACHKV2_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6943156.1"
                     /translation="ALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGGGTVVVVGRQG"
     gene            898..>1190
                     /locus_tag="ACHKV2_005500"
     CDS             898..>1190
                     /locus_tag="ACHKV2_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943157.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKA"
BASE COUNT          289 a          271 c          380 g          250 t
ORIGIN      
        1 gcgctgtcgt tacaggtgcc gggctttcgc cgtcagatga acgaaggctg gtaccagata
       61 cgtattgccg gttatgacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg
      121 ggtgagggaa cggtcatcca tattgtgccg cgactggccg gggccggaaa gggtggactg
      181 cagattgtgc tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
      241 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
      301 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca
      361 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
      421 atgattgccc aggggaaccc gatgccggtg ccttacgggg aaatgctggt tggctcccgc
      481 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaacggt cgtggttgtc
      541 gggcgacagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
      601 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
      661 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga atttttatgc
      721 cattaccggt tttaaccaac aggattatcg gtgggcatga aagaaaaccc cggtatctgc
      781 tgataccggg gtttctcttt agcatggcag aaatgtgttt catgcttttc gggcgaagga
      841 tatccgactt ctgtacggaa tggcaagtgg cggttaattt attcagggga aggctgtatg
      901 ggaaaaggtg gcggtaaggc acacacgcct cgtgaggcga aggataatct caaatccacg
      961 cagatgatga gtgtgattga tgcgattggt gagggaccga tagaaggtcc ggtgaaggga
     1021 ctgcagagta ttctggtgaa caaaacccca ctgacggaca cggacggcaa tcccgtgata
     1081 cacggtgtga ccgcggtctg gcgtgccggg gagcaggagc agacaccacc ggaaggcttt
     1141 gagtcctccg gagctgaaac cggactgggc gtggaagtga cgaaggcaaa
//
LOCUS       DAZFTN010000367         1184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFTN010000367 DAZFTN010000000
VERSION     DAZFTN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..261
                     /locus_tag="ACHKV2_005501"
     CDS             109..261
                     /locus_tag="ACHKV2_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6943158.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            742..930
                     /gene="dicB"
                     /locus_tag="ACHKV2_005502"
     CDS             742..930
                     /gene="dicB"
                     /locus_tag="ACHKV2_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6943159.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            927..1115
                     /locus_tag="ACHKV2_005503"
     CDS             927..1115
                     /locus_tag="ACHKV2_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6943160.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
BASE COUNT          332 a          263 c          283 g          306 t
ORIGIN      
        1 ttaagtagcc agcccgaggc gtatgaacat gacggcggga acactttgta taacagcgca
       61 gcaggttttt agttccgcga cccggcgtta agggtaaatg aggtcaacat ggatatgctc
      121 aatcttggca acaatgaatc tctggtgtgc ggagtattcc ccaaccacga cggcacgttt
      181 accgcgatga cgtataccag aagtaaaacg tttaaaaccg aagctggcgc gcatcgctgg
      241 ttagcaagaa acgctaactg attagcgcca gtaaaaacag gtttccacag gttaatttac
      301 cctgaaaagt cagggcataa cacgaaagcg cacggcgaag ttagtctctc tgtataggtc
      361 gtcgttaaat ttaattcgac cgtgcgcttc tggttgtggc aatccgcgaa atggcgcggc
      421 ggtaagtatg gcggggttat tccttccccc gttgaggaca ccgggttgtc aggttgacca
      481 tacgcttaag tgacacccca gccacaacaa cctctgttat cacttttctg gtgattcggc
      541 ggaaatggat atccgccctt tttaaagtga attttgtgat gcggtgaatg cggctatgcg
      601 cacgcggaac agttaaagca gtaaggcggt cttttactgg cgtaacgagc atcaactaac
      661 ccgacgttaa ttgttaactg gttaacgtca cctggaggca ccaggcactg catcacaaaa
      721 ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac gtctgaaggt
      781 tgttttgata ttggtgttct gctcagtaat aaagcgttta ctgaggatgc cattaatatg
      841 cggaaatatg agccttatct gctcaatgat aattccattc tttcccgaat tgcccttatt
      901 aaacttggta ttttcggaga acgtcaatga cttcagcatt tgcactggtg atgacggttt
      961 ttcttataac gggtgaatca cagaatgtta ttaccggaat ttatgccagt aaagaatcct
     1021 gcctccaggc aagagacgag caaaaaattt ctggtgaatg cctaccggta aaaaaagtat
     1081 cgctgtacct gaataacgaa acaccggctg gataacccgc cagccatatt aacgccatac
     1141 caacggatta aaaatgccag caatggcagg gattcgttca ccct
//
LOCUS       DAZFTN010000368         1174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFTN010000368 DAZFTN010000000
VERSION     DAZFTN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..137)
                     /locus_tag="ACHKV2_005504"
                     /pseudo
     CDS             complement(1..137)
                     /locus_tag="ACHKV2_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            287..955
                     /locus_tag="ACHKV2_005505"
     CDS             287..955
                     /locus_tag="ACHKV2_005505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6943161.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          353 a          249 c          220 g          352 t
ORIGIN      
        1 gctactgatg tatcaacacg attcaacaat acccgatatt tattccatgc ctccagcaac
       61 gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg cgcaatatac
      121 tcactgaatt cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc
      181 tgtatcgcag cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt
      241 gtaattcatc gccataattg atttaattca caaataaaac tataacatgg tgaaattaat
      301 gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga
      361 ctggtgggtt ttgggagtat caaatcgtct tgcatgggga tgtcctacaa aggaacacct
      421 tcttccacac tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg
      481 gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc
      541 tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca
      601 tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt
      661 ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc
      721 ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg gcgatggagt
      781 tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc
      841 aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt
      901 taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc
      961 aaccgcagca cgttcttgca tacgccgtgt ctgcggttta atttttactc tcaaatattg
     1021 tagttaagta caataatgat tcctgtggct atatagatgt ttttgtaatt agtccttaag
     1081 ataaaatttc tctatttatc caagaaacag acttcattag gaagagtcgt aaaaacatta
     1141 acaactaccg ccgtgtaatt aaattatttt ttag
//
LOCUS       DAZFTN010000369         1167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFTN010000369 DAZFTN010000000
VERSION     DAZFTN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..858
                     /locus_tag="ACHKV2_005506"
     CDS             <1..858
                     /locus_tag="ACHKV2_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6943162.1"
                     /translation="GIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRSAFTA
                     LQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYSPAEFR
                     KKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGRKVLI
                     SYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAEEKIR
                     DAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGLQLLEA
                     KLKENDPLA"
     gene            929..>1167
                     /locus_tag="ACHKV2_005507"
     CDS             929..>1167
                     /locus_tag="ACHKV2_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6943163.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTV"
BASE COUNT          327 a          274 c          330 g          236 t
ORIGIN      
        1 ggcattatcg aacggcttaa ccgcacatta ccgatgcgca tagcccgtaa ataccgcacc
       61 tatttcggga aaggtgcaga tcgcgagacg ttacgcaaaa ccaaccgcga tttacgctcg
      121 gcatttactg ccctgcaaca gggcaaacgg ctgaacgccc ggcagcagtc agcgatgcgt
      181 gatttaccgt cctggtctga actgattgat gccattcgtg atggtgttga gtggtacaac
      241 aaccggccgc acgatgaatt accggtgaag ccgaacggca agcattacag cccggcggag
      301 ttcagaaaaa aacgcctggc ggaagaggac acggaaattg aatggctgtc cgatgtggaa
      361 ttgcgggaca tgttccggcc gatggtggaa cgccctgtaa gacgctgtga aatacgctgg
      421 ctgaataata tttactacgc gcccgagctg cgtgatgagc atggccgcaa ggtgcttatc
      481 agctatgaca ttcatgatgc cgaacgaatt accgtacgtc gcctggatgg cagcgtgatt
      541 tgcgaggcgg tatgggacgg taataaacgc gaagccttcc cggttagcgc ggaatactac
      601 aaacagcagc aacgccttaa aggcatgcgt aaacgcgcag aggaaaaaat ccgtgatgcc
      661 gaggatgagg ttgtcaacgt gctggagcac aagccgcagg agccctggct ggaaaacatc
      721 tatcgcccgg taggtaatac ggtggccgtt cagcaacctg ccgttgatga tgagcctgat
      781 gaagaatacg agcgcaattt ccagcgggga ttgcagttgc tcgaagcgaa attaaaagaa
      841 aatgacccgc tggcctgaaa taaaaaataa cccgagcggc gactcaggtt atttgattaa
      901 acaaggtatc aaatgagagg ttaataatat gactgatatt aacgatgtaa tcaagaccat
      961 tgatgaactt attgatggcg gcgtactgac gcagtatgcc atcgccagag aggcgggaat
     1021 ttccgacggc acattatcgg ctttccgcaa ggggaaatat aaaggcgata acgccgctgt
     1081 ggctgcttcc ctgcgttcct ggtatgagaa ctggaataaa caaagcgcac tgccggaacc
     1141 gccgcagttt gtggaaacgc agacagt
//
LOCUS       DAZFTN010000370         1165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFTN010000370 DAZFTN010000000
VERSION     DAZFTN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..1106
                     /locus_tag="ACHKV2_005508"
     CDS             147..1106
                     /locus_tag="ACHKV2_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO6943164.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          416 a          219 c          217 g          313 t
ORIGIN      
        1 taaaagataa taaaaaccgc aataaaaaac gaataaatca attgtctcac gcaattataa
       61 aacatcatat tgattacgca ccttgcatta caaactcatg tatgtaaaat acgcgcacca
      121 ttcaaaaaaa aggaagacaa taacatatga aaaaaagtgt catcgctggc gtctttattg
      181 ctctgtcatt taccacgtgt tcagctatcg cgaacagcct tgcattatca ttagcaaatg
      241 atgatgcagg gaagtttcaa ccaatactta atgatattta tggcaataaa catgaaaaca
      301 gagatgatta ctcacaaggc ttatttctgg gatatagcca cgatatctca gactcgagcc
      361 aattatctct ccatattgcg caagatattt actctccatc aggcagtaat aaaagacaca
      421 acacagctgt aactggagac agagctttta gtgcatacac tcacactggt attgaatgga
      481 actcccttgc gaatgactgg attcgctatc gattaggtac tgacataggt gttgttggcc
      541 ccgacgcagg cggtcagaaa gtacaaaata aagctcatga gattattggg gcagaaaaat
      601 atcatgcatg ggatgatcaa atagagaatc gctacggtta tactgtaaaa gggatgctat
      661 ccatgacacc aagtatggat attttaggtg ctaatgttgg attataccct gaagtttctg
      721 ctgttactgg aaacttattt caatatgtag catatggcgc aaccattgcc attggtaatg
      781 ataaaacctt caattcggat aatggctttg gtctgctggc tccccgtggt ttaatgcata
      841 tgtccgatac aagcggattc aaatacaaga tttttgcagg tatggaaaga cgagatgtca
      901 atcgcaacta tactctcgaa ggaaaaacaa tacagacgaa acaaacaaca gtatcgctaa
      961 acaaaactgt tgatgaatat caagttggcg caacaattgg gtatgcacct gtagccttca
     1021 cactagcatt taataaagta acatcagaat tcaagacagg ggatgactat tcatttataa
     1081 atggagcaat caccttcttt ttttaactga attgaattca atcaaaataa cataagtcca
     1141 acaaaaacat aaagtgcgaa atgaa
//
LOCUS       DAZFTN010000371         1155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFTN010000371 DAZFTN010000000
VERSION     DAZFTN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            650..1119
                     /locus_tag="ACHKV2_005509"
                     /pseudo
     CDS             650..1119
                     /locus_tag="ACHKV2_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          333 a          220 c          248 g          354 t
ORIGIN      
        1 cggcatgcca gttaacagct atccgtatgg cgtgttcggt tttgcgaata aaaaatggtt
       61 actgtagaag cggatttcca ccacatgtca accatgaatg gtcagtgatg cgagacgact
      121 gggatgattt ggtcgatgac ttcttccggc gaatactgat agaaacattg aaatattgta
      181 agagaaagcg ttcaaaattc tgtggcacgt gaaaaattct acagcgtcat caccttattc
      241 agtcgacctt caaaataaac gtttaacaga gacaatcacc atttggattg tccgctgagg
      301 aggcgaacaa tttcctagac agctttatca tgtcagccca cattgttggc gctggcaata
      361 ctaaggacaa acgatgggga agtagctttt tagcgttatt ttcgggagta aaaaacgtgg
      421 tatctttccg tatgtcagtt gtatcttgct ggtattcgta gtcgtgataa ggcatacaac
      481 gatatttatc ttgacgttag tgaagaataa gttgttttgt gatctatgtt aatctgggat
      541 tacaatccgg gcttgataca tcgcatcacg acttttattc tcttcagaat agaatatgtt
      601 actccccgct ttacaggctc aggatttgaa ttacaaatta tacttttaag tgaacaataa
      661 ccaagggtat ttcttatttc ctgaaaccag gcttggccca tttcgggatg agtttgaaaa
      721 aactcttgac gcatacgata gtgatgaaat aaacgaaaca aattacgtca acaaactcag
      781 gcgtcttgcc tgacatgctc ctgacatcat tgatattcac gctcatctgg catatgcatt
      841 tgcgctcttt atgctgcaat tctggcgaat gttcatttac agcgtcatca ggatgccgtt
      901 atactcattg ataaaatact aacatataac cctgagaata atcagggaac acgctggtta
      961 ctgggggcgg aattgttacg tgctggcaag catggcaggg ctctgtacgt attgacagga
     1021 catgtcaatg aatttccccc ctactggtac gagcttgctc ttttacattt tatgaatgag
     1081 gcatatatta atgctgcaac ggcatttcga tatggatttt taacaagtat atattgttga
     1141 aatgctttgt tagaa
//
LOCUS       DAZFTN010000372         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFTN010000372 DAZFTN010000000
VERSION     DAZFTN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1022)
                     /locus_tag="ACHKV2_005510"
                     /pseudo
     CDS             complement(1..1022)
                     /locus_tag="ACHKV2_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          375 a          210 c          177 g          391 t
ORIGIN      
        1 tttgtttaat tcactgtcgc ttaacgtacc aatgatattg aaatacacat tgttagctgt
       61 tttatatttt tcataagaaa gacctattgc agacaatgtt tcaatagcct gactttccaa
      121 tacattatca tctaataccg caatacttat attgctggca tatggcatag tttgtagtaa
      181 ccctttaata agattgtcga ttagttgctg tgtgatagca ttccgttgct tactcacggt
      241 cagtctggga ttactggcat ctaatagctg aagtttaaaa tattgtaaat cagggaaatt
      301 atcagcaagc catgaaaaaa ttcgtatcga ttcatcatca ggatttatca cccgtactgg
      361 ctttcctggt tgattttttt ccagtgcttg tttgacccag actgcttcat ccaaactatt
      421 ggtattgata tagatgacat gatctctacc atataaaatt tcataattcc tgtttttgtt
      481 tacaagaatc ttatcgatat gtctcagctg cggatcatta ctggcattaa caatataaaa
      541 agcaatgaaa atgataacaa aaagaaaaaa aagcattaca tactttaaca gtttactgct
      601 aaagaaatgt tgttttgtag aaataatgct attttcaata atgcctggca atgaatactg
      661 ccaaacttca ttttttcttt tacagcaaat ttgagaccta aaacttctat ttgctgattc
      721 aatgttaacg ttctatcttc aggttcttgt gctgttaatt ctctgattgt tacaaccaga
      781 tcagggtcgg tagtgattat aatctcaaaa ctacctgtaa gctcgccata aggaacgact
      841 attgtatttt ctggtaactc agaaaaagca tcacttcttc ctaacgaaac atcattacca
      901 atgataacaa gtagacgccc attaagtatt tcatactcgc atccattcta tggtccgctc
      961 aacaaacgaa tggcataaga ttccaataag tcttgtgata agaatttatc attattttcc
     1021 atgaatacca cccgctaatt ccataagctc tagtttagag tagtgtaaca agacggaaat
     1081 ttcatgacca aattatgatg agacagaata aaaaaattat aattttgagg accattttaa
     1141 atgcctctat gca
//
LOCUS       DAZFTN010000373         1151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFTN010000373 DAZFTN010000000
VERSION     DAZFTN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..605
                     /gene="espM2"
                     /locus_tag="ACHKV2_005511"
     CDS             15..605
                     /gene="espM2"
                     /locus_tag="ACHKV2_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO6943165.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            839..991
                     /locus_tag="ACHKV2_005512"
                     /pseudo
     CDS             839..991
                     /locus_tag="ACHKV2_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          371 a          209 c          271 g          300 t
ORIGIN      
        1 aaaaaggaat tataatgccg atgaatacta caggtatgtc tttcagctct tttggtataa
       61 gttgccatag agagaactcc ttcaggaact ctttccgagg gaagaatgat gaggtcgtaa
      121 aatgttcaat tggagaacgg actattagct tttctgttcg caaatttagc ggcaatatac
      181 tggaaacggt gagaaggcag agtactaaag atattgatga gtggataaaa gatgaacgga
      241 tagtatatcc ctcaagggtg atcaaccagg agattgataa ttactgtttt caaaaaaatg
      301 caaaaatttc cactgaagag aggcaaagag tcttttttct tgtgagccag gagaatcaac
      361 taacccttga tgttaaggca gcacaaagtt ctattaatca tgtaataatg gggagtgctt
      421 cttttggcaa gaaaatggat gctctttgtg atggtatgag ccgggatgta aaaaatcgta
      481 catcagatac catagcaaac ttgcttgcgg acaagtttta ccagaaacat atcgattcgg
      541 atattgatat tgtaaaacta cgaaatgata tcccagatta tttgatgcgt gctatacagg
      601 gatgaggcta gaggagagga ctgcttcagc atcatcggct tcggaccagt aaaagagacg
      661 cagaaaatgc acaacggcac cacacgccat gcatggattc agattactca tggctcacca
      721 gtacagctat aaatcgtaaa gaaaactaca gtacgttata cacagaaacg taccgtgatt
      781 cattatttat tgtacaggtt catcgcagcg acaatatttt gtgatgtttg cgtggtgcgc
      841 cccacttcag gatgctgcag atctggaaat tgcaacggag gaagaaacct cattgctgga
      901 agcctagaaa aagtatcggg tgttgctgaa ccgtgttgat acatcaactg cacctgatat
      961 tgagtggccg gaagaaccag acacaatgta agcgaaaaag aaaaaccgca gacacgacgt
     1021 atgcaggacg tgctgcggtt ggctggtgaa ctttcgatag tgcgagtatt gaatgatttc
     1081 cagccgttac cgattttacg tgttaattag tgaacaaacc actcgtcagc agacttccag
     1141 gtatctttca g
//
LOCUS       DAZFTN010000374         1151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFTN010000374 DAZFTN010000000
VERSION     DAZFTN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..361
                     /locus_tag="ACHKV2_005513"
     CDS             122..361
                     /locus_tag="ACHKV2_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO6943166.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            381..713
                     /gene="sctI"
                     /locus_tag="ACHKV2_005514"
     CDS             381..713
                     /gene="sctI"
                     /locus_tag="ACHKV2_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO6943167.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            710..>1151
                     /gene="sctJ"
                     /locus_tag="ACHKV2_005515"
     CDS             710..>1151
                     /gene="sctJ"
                     /locus_tag="ACHKV2_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO6943168.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHIS"
BASE COUNT          375 a          203 c          240 g          333 t
ORIGIN      
        1 agatttaatg tgaatttgaa aaatcaggac acaaataata gttcatttag ctacggagat
       61 aaccgattcg agaagtctca aggtagcaag tggacatttc aggaataatt gaggaggact
      121 tatggcagat tggaatggtt atattatgga catcagcaaa caatttgatc agggcgttga
      181 tgatctgaac cagcaagttg aaaaagcgtt ggaggattta gcaaccaatc cttccgaccc
      241 gaaattcctt gccgaatatc aaagtgcatt agctgagtat acattatatc gaaatgcgca
      301 atctaacgtt gtaaaagcgt ataaagatct tgattcggca atcatacaaa acttccgcta
      361 aactggataa ggattttatt atgtcagtaa gcaatatgcc acctattgat agagctgagc
      421 aatcaactgc tcatgaaata cagcaagcga aagtgattga tttaaatgac cgggttctca
      481 atcttgacaa cccggatgat aaaatgatct ccgcttttgc taactatgct gtacaaactg
      541 aaaattggca gcaaaatgcg ttacaagcat taacgtctga taaaaaagga ttaactcctg
      601 aaaaattatt agtcttgcag gatcatgtgc ttaattataa tgtcgaggtt tcattggtgg
      661 gaacattagc aagaaaaatt gtcgctgcag ttgagacgtt gacccgctca tgaaatatat
      721 atcattatta ttgtttatcc tgctgttgtg cggctgcaag cagcaggaat tactgaacca
      781 tcttgatcaa cagcaagcta atgatgtcct tgctgtttta caaagacata atattaatgc
      841 tgaaaagaag gatcaaggca aaactggatt ctcaatctat gttgaaccaa cggattttgc
      901 ttctgcggtg gattggctaa aaatctacaa tctcccgggt aagcctgata ttcaaatatc
      961 gcagatgttc cctgcagatg cactcgtttc atctccacga gcggaaaaag caaggctata
     1021 ttctgctatt gagcagcggt tagagcaatc tctcaaaatt atggatggta ttgtttcttc
     1081 gagagtacat gttagttacg atgttgatac tggcgatagt ggtaaaactg ctttaccaat
     1141 tcatatttct g
//
LOCUS       DAZFTN010000375         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFTN010000375 DAZFTN010000000
VERSION     DAZFTN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            132..239
                     /gene="yniD"
                     /locus_tag="ACHKV2_005516"
     CDS             132..239
                     /gene="yniD"
                     /locus_tag="ACHKV2_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO6943169.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(292..1050)
                     /locus_tag="ACHKV2_005517"
     CDS             complement(292..1050)
                     /locus_tag="ACHKV2_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO6943170.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          311 a          280 c          268 g          271 t
ORIGIN      
        1 tttcataaaa cgcagaaaca gtgatcaggt gtacaacgcc acccgacggc tgtaataaca
       61 aaaaatccct tgatgcctgt cccttttgtt acactccgtt atcacgcaca agagatatgc
      121 aggacactgg tatgccgact aaacgctttg ataaaaaaca ctggaagatg gtggtggtgc
      181 tactggcaat ctgtggcgct atgttgttgc tacgttgggc agcaatgatt tggggctgag
      241 cagtgacaaa cgaagacagc cagaccgcat aacacggtcc ggcagataat tttacatgct
      301 gtagcccagc gataaggttg tccgacggtc ggtatgttcc ggtgcagact ctggaggttc
      361 agagttccat gtgacgttgt aagccacctt cagtccaaaa tgctcattaa tagcaacatt
      421 taatgcgctt tcggagttca gcgttgtgtc ttccgcgcca aagacggaaa caccctgcgt
      481 aaatttagcg ttgtcagtaa gctgccatgc ataagcaccg gaggcatagc ccagcggctg
      541 agtttcactg gcattgtcgg tatatttgtc gtaacgcaca cctggaccga attcaaagcg
      601 gaaactgtgt accgggccat tcaaaaactg acgaccataa cccgcggtca acacatcgcg
      661 ctctcgatag ccgttataac ggtctgtcag ccagcttgcc tgaccaaata aatagtcata
      721 atcagttaaa ttaaaacggc tacgcccgcc cgccgcatat ttttctgaag aacgctcatc
      781 attagaagaa gtattactgg cgttccccca cagcgaccag gcagtggttt gtccatacca
      841 ggtcatggtg gtgtcagccg taagtgagga gcttttcgta ttgcctgatt gtgcaagata
      901 ccctgcgttc agattacctt cgaaaggttt tttagcgctg gcaggatcgt ccatgacagt
      961 aaaaacggaa tcatcggcag ctgcattcag tgacgcaaac atgccccccg ccaacataac
     1021 gatggcagga actgtcttca aaagcttcat ttatcaagag tccgtacaac aaaaaaagag
     1081 accatcgcgg tcccggaaac tttcttaagg atcaaagatt agcgtccctg
//
LOCUS       DAZFTN010000376         1119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFTN010000376 DAZFTN010000000
VERSION     DAZFTN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..447)
                     /locus_tag="ACHKV2_005518"
     CDS             complement(76..447)
                     /locus_tag="ACHKV2_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6943171.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(437..808)
                     /locus_tag="ACHKV2_005519"
     CDS             complement(437..808)
                     /locus_tag="ACHKV2_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6943172.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(821..>1119)
                     /locus_tag="ACHKV2_005520"
     CDS             complement(821..>1119)
                     /locus_tag="ACHKV2_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6943173.1"
                     /translation="SFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          312 a          311 c          210 g          286 t
ORIGIN      
        1 cacaatacat gaccatcaaa aaataaacat gttaattata aacacagaaa atataaccct
       61 catcactata tatccctacc gcatatccat atctaaccgg acattcagag ccataagcat
      121 tccttcaata ataccttccg ctttataaag ccttttacca ataagcccat cagaacatct
      181 atgcttacgt gcaagagcca taaatgtcat tccacctacg taataatcca ccaacaaatc
      241 gtgcaaatac tgattattct tgtttaatcg tgccatacaa ccacaaatta tcatggcgtc
      301 atcatcagaa cacttaggac gtgatttcac tttagtcggg attaatcctt taaaaccagc
      361 agctatcgag ggccatgtta catcttcatg attatttgct gcccatgccc cccatcgctc
      421 aagaaccatc tggatatcac gcgccatcgt taccacctct gatttcgtaa atcttcacgc
      481 ccagccgtcc acctggcaca ggctgaccgc gcacaatatt gatttcatca aactgctcgt
      541 catcaatgag cacttccgca tgcgtcagcg catccagcgg tgctttcaga atgttgtcca
      601 ggtcacggcg gcgcttatcc ggtggttctg caataatttt tattgccagc cgtccggaca
      661 ggcttaattt cagccgctgc tggcgaacaa taagcgccac agcccggcga taacgctcac
      721 cggcttttga tacaaaatat gtgctgccac gacgtcgcca gtaggtgttc accgtcggcg
      781 ggtaaggcaa aacaaactct atacgcatca gtaacctctt ttacccgagc acgccggttg
      841 caaaagcgtg atcaagaaaa cgaaaaatta aatcaacctg ggaaccatgc ttttcttcga
      901 acgccagcgg atccgcatga agctcgttgt gatgctcccg acacagcggt agcgtgaaaa
      961 tatcgtggga ttttgttccc attccaccct gaccatgacc aatcagatga tgaggatcgt
     1021 ccgctggctt accacaacac gcacacggct gtgtctttac ccagcgtgtg tatttctcat
     1081 ttacccagcg gcgacgttta ggtcgtttca tgaaggatt
//
LOCUS       DAZFTN010000377         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFTN010000377 DAZFTN010000000
VERSION     DAZFTN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..274
                     /locus_tag="ACHKV2_005521"
     CDS             <1..274
                     /locus_tag="ACHKV2_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO6943174.1"
                     /translation="KLAQVIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPD
                     LYGSRVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
     gene            557..778
                     /locus_tag="ACHKV2_005522"
                     /pseudo
     CDS             557..778
                     /locus_tag="ACHKV2_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
     gene            complement(775..>1107)
                     /locus_tag="ACHKV2_005523"
     CDS             complement(775..>1107)
                     /locus_tag="ACHKV2_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943175.1"
                     /translation="TSGSSNGSYSNLGSHRGSFTGRNTGSGTLFVYASGGNGGSAGGD
                     CANTSRLQGYVAGALISTNASNNPSYGKTAFISFAVPAGATYQITSYPAQNYSCGSGV
                     FSVFGYQT"
BASE COUNT          293 a          267 c          268 g          279 t
ORIGIN      
        1 aaaattggcg caggtgatag ccaaattgtt accgcttcag ctactgcgtg gcgttggcct
       61 ggagcaacgg caacatgtcc atctggcaag aaagtcattg gtggaggcgg tcaatgcaga
      121 tcaaataccg gatttatatg gctcacgcgt tccatgcctt caggcaacaa tgcatggact
      181 gccagttgcg acacgaccga agatcaaaat ggttcaatta cagtttacgc catttgccag
      241 tgacgatact tatcactggc aaaccacgat gtaactacat ttccccgaca ggtcagttcg
      301 gtgagtaccg ccataaaagt tgtattcgtc atagaagccg catttcacaa ttcgattggc
      361 atcataggtt gcaactgacc ccgcaaaata attcggcttc cacccgtgcg gattttctgc
      421 attactgaca ctccaggtac actggctggc agaaaaacca gatggcaatg ggatctgttg
      481 tccatttgct atttttcctg tcagaacagt gctacctgat gcccttctcc acgtaccgga
      541 ttggcacgga ggccagtgcc aatccggtgt gtggagggca ttaggtggaa agctcaaggt
      601 tactcaactt tccagcactg gttacttggg gcaatttgac ttttgtgcca ttgccagaat
      661 gggaaatgct gaggattctc attactgcca ggtagtcgag agtccatctg gttcacgaaa
      721 atggtacaaa tacgagcaca agacaggttg tattgcgtcc tgtgtaactc tcaattaagt
      781 ctggtatcca aatactgaaa atacccctga gccacaagag tagttttggg ccggataaga
      841 ggtgatctga taagttgcgc cagcagggac agcaaaagag atgaatgctg tcttcccata
      901 agatgggttg ttgctcgcat ttgtgctaat caatgcccct gcaacgtatc cctgcaatct
      961 ggatgtgtta gcgcaatctc ccccggcgga accaccattc cctccagagg cgtaaacaaa
     1021 cagcgtacca cttcctgtat tgcgaccagt aaatgatcct ctgtgtgagc caagattact
     1081 gtaactaccg ttagaggaac cggaagt
//
LOCUS       DAZFTN010000378         1094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFTN010000378 DAZFTN010000000
VERSION     DAZFTN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1094
                     /locus_tag="ACHKV2_005524"
     CDS             <1..>1094
                     /locus_tag="ACHKV2_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6943176.1"
                     /translation="DALAQKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVV
                     KLTGGEGAVISATRDPEYYFIVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRST
                     VTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALK
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSADGVPW
                     ICGDMTYFWKQKNEATYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIP
                     GIGYYGSKWRDSSATWTSQDRASHFSAWARR"
BASE COUNT          268 a          258 c          342 g          226 t
ORIGIN      
        1 gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat acggtgggcc gttttcttcg
       61 tatacggatg atggcgcagc acttattcag gcgattgtcg cagaaggtga tgtgagcaca
      121 cctgttgtgg tgaagctgac aggtggagaa ggtgcagtaa tcagcgccac cagagacccg
      181 gagtattact ttattgtggt tctggcgggg cagtcaaaca gcatggcata tggtgaaggc
      241 cttccgctgc cggagacata tgaccgtccg gacccgcgta ttaagcagct ggcgcgccgc
      301 agtacggtga caccgggcgg tgtcgcctgt aaatataacg acatcattcc ggcggaccat
      361 tgtctgcatg atgtgcagga catgagccgc cttaaccatc cgaaagcgga cctgtcaaag
      421 gggcagtacg gaaccgtggg gcaggggctg catatcgcca aaaaattgct gccgtttata
      481 ccggcgaatg cgggcattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc
      541 ggagctgatg gcacatacag tgacgcgagt ggtgcttcgg agaattcaac ccgctggggt
      601 gtggacaagc cgctgtataa ggaccttatc ggtcgaacaa aagcagcact gaagaagaac
      661 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
      721 ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      781 gatatggcag gtcagtgcgt cggtggctct gctgacggtg ttccctggat atgcggggac
      841 atgacgtatt tctggaagca gaagaacgaa gccacctacc agacggtgta cggcagctac
      901 aaaaacaaaa cggaaaaaaa tatccatttc gtaccgttca tgacggatga gaacggggtg
      961 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
     1021 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagc
     1081 gcctgggcac gccg
//
LOCUS       DAZFTN010000379         1087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFTN010000379 DAZFTN010000000
VERSION     DAZFTN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..152)
                     /gene="cesF"
                     /locus_tag="ACHKV2_005525"
     CDS             complement(<1..152)
                     /gene="cesF"
                     /locus_tag="ACHKV2_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFO6943177.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCY"
     gene            418..1029
                     /gene="map"
                     /locus_tag="ACHKV2_005526"
     CDS             418..1029
                     /gene="map"
                     /locus_tag="ACHKV2_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFO6943178.1"
                     /translation="MFSPMTMAGRSLVQATVQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          360 a          194 c          231 g          302 t
ORIGIN      
        1 aaataacaat aaaaaaacag aatttcagat tgagatgcac actccagtat ccattcatgg
       61 ccatcgttat cgtggaaaga gagcacgtca gtttgctctt caagagctat cccaaacgta
      121 tgtgcaaggt ctgtgcgaaa tttctcattc atattgtagt cctgccattc ttttcatgtt
      181 gatatactca aacgtaatta atccgagagt tctgatacaa ataataaggc gtagatataa
      241 gattacggga atagtgttgc atattcatga tagcatatga taaatgtagt gcaactacct
      301 gtagataagg ccggaggata attcaaatat aaatacaaga taattattct ttaaagttat
      361 tttttaatgg gaagcaattt ttttactgtc gaaatataat ttataaaggt ttatggtatg
      421 tttagtccaa tgacaatggc aggcagatcg ttagttcagg cgactgtaca gacgcttaaa
      481 cctgcggtta ccagagctgc aatgcaagcg ggtacgggag ccacgggtat gaggttcatg
      541 cccgtgcagt cgaactttgt gattaatcat ggcaaactga ctaaccaact cctgcaggct
      601 gtagccaaac aaactcgtaa tggcgatacc caacaatggt tccagcagga gcagacgact
      661 tatatatcca gaacagtaaa tagaactctg gatgactatt gcaggagtaa taattcggtc
      721 attagtaagg agactaaagg tcacattttt agagctgttg aaaatgcgtt acagcaacct
      781 cttgatatga acggggcaca atcatcaatt ggtcacttct tgcagtccaa taaatacttt
      841 aatcagaaag ttgatgagca gtgcggcaaa agagtggatc caataacacg ttttaacacc
      901 cagacgaaga tgatagaaca agtctcacaa gaaatatttg aacgcaactt tagcggcttc
      961 aaagtcagtg agataaaagc gattactcag aatgcgattt tagagcatgt acaggatacc
     1021 cgattgtagg gataaccttg tcaggtataa attaagcttc ctggtgtata gcatggcctt
     1081 tgttgat
//
LOCUS       DAZFTN010000380         1069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFTN010000380 DAZFTN010000000
VERSION     DAZFTN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..415
                     /locus_tag="ACHKV2_005527"
     CDS             50..415
                     /locus_tag="ACHKV2_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6943179.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
     gene            705..>1069
                     /locus_tag="ACHKV2_005528"
     CDS             705..>1069
                     /locus_tag="ACHKV2_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6943180.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRL"
BASE COUNT          254 a          262 c          317 g          236 t
ORIGIN      
        1 gatgacattt tctttcctct ggtgacaagc tatatggcga ggattttaca tggctgtgct
       61 tcgtacgtta ccgggcagaa tcaaaactct gaacacccgg cggataaatg tcctgagggg
      121 ggaacagcgt cgtgtcagtg gcagtgcccg ggtttccctc aaacgtcgta tctggcggag
      181 ggatgccgga cactgttgtc tctgtagacg cgtggttgac ctttgtgaca gtgaactcga
      241 tcaccgcatt gcacttcagt tcggtggtgg taatgaggag acgaacctct ggaccctctg
      301 tactgaatgc catcgccaga agtcagcgag tgaagcggcg agtggtatgc ctgatccgac
      361 gttgcctgag cttcctgatg gcacgctcag ggccgacgga atcactggcc tgtgaccaga
      421 cccggggggg atcatccggc gaaaaaaaac gatcgccctg gacaccgcgc cccctctcac
      481 gcagagaaaa aattcccgtt tcagggcagt taacatgtta actggctgcc cgggcatttt
      541 tgcggttttt atctttatta ttcagtttgt tgtgcggaaa aaatgttaac aggctttttc
      601 agcaaatgtt aaccaggcag cagttaacat ttgcggcatg agacgccggg aaaaatgggc
      661 tgaaccatac ccggctgagt gcgttctgga cccgggagga ggctgtgctg acaacgcaaa
      721 aacgaaaatt tgcgctggcg ctcatgtccg ggaaaaacaa aacagcgtca gcccttgccg
      781 ccggttattc ggcgaagaca gccagggtta aaggctcgca gctggcaaaa gatccggagg
      841 tgcttgcgtt tatagcccgt aaacagtgcg agacggtgga ggtggatgag gttcctgttt
      901 accggcagaa aaaatcagag ccggaggata aaccccgtcg ccgtgaggcg gctgcaatac
      961 cacagccgga cgaaacaaat ccggagatgc caccgcccgt ggtgatatct cctggtattg
     1021 agtatatgga ggacggtctt cccgatccgg tgaaagcgat ggggcgtct
//
LOCUS       DAZFTN010000381         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFTN010000381 DAZFTN010000000
VERSION     DAZFTN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKV2_005529"
     CDS             <1..615
                     /locus_tag="ACHKV2_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6943181.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
     gene            826..901
                     /locus_tag="ACHKV2_005530"
     tRNA            826..901
                     /locus_tag="ACHKV2_005530"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:859..861,aa:Ile,seq:cat)
     gene            909..985
                     /locus_tag="ACHKV2_005531"
     tRNA            909..985
                     /locus_tag="ACHKV2_005531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:943..945,aa:Arg,seq:tcg)
BASE COUNT          293 a          219 c          282 g          265 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg gtgaatgcgc tggtggagga agcaagggta atttttaacc aggtgattct
      781 tgaatgcttg caacattgat ttcgtaacgt tattatcctg cgcccggccc tttagctcag
      841 tggtgagagc gagcgactca taatcgccag gccgctggtt caaatccagc aagggccacc
      901 atcacatacc gccattagct catcgggata gaacgccagc cttcgaagct ggtttcgcgg
      961 ggttcgagtc tccgatggcg gtccattatc ggtattctgc gtttttagct cagccggaca
     1021 gagcaattgc cttctaagca atcggtcact ggttcgaac
//
LOCUS       DAZFTN010000382         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFTN010000382 DAZFTN010000000
VERSION     DAZFTN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1059
                     /locus_tag="ACHKV2_005532"
                     /pseudo
     CDS             1..1059
                     /locus_tag="ACHKV2_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
     misc_feature    252..368
                     /locus_tag="ACHKV2_005532"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          248 a          250 c          332 g          229 t
ORIGIN      
        1 gtccgtcagc gggcgattcg tatggttctg gaaagtcagg atgaatatga ctcacagtgg
       61 gcggcaattt gttccattgc cccaaagatt ggctgtacgc cggagactct gcgtgtctgg
      121 gttcgccagc atgagcggga taccgggggc ggtgatggtg ggctcaccag cgctgaacgt
      181 cagcgtctga aagagctgga acgtgaaaat cgtgaactgc gccgcagtaa cgatatcctt
      241 cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa aaaatgatgc
      301 cactgctgga taagctgcgt gagcagtacg gggtcggacc ggtatgcagc gaactgcata
      361 ttgccccgtc aacgtattac cattgtcagc aacagcgaca tcatccggat aaacgcagtg
      421 cccgtgcgca gcacgacgac tggctgaaga gagagataca gcgcgtatac gatgaaaatc
      481 atcaggtgta cggtgtgcgt aaagtctggc gtcagttgtt acgggaagga atcagggtgg
      541 ccagatgtac agtggcacgt ctcatggcgg ttatgggact tgccggtgtt ctccggggta
      601 aaaaggtccg tacgaccatc agccggaaag ccgttgccgc aggcgaccgc gtaaaccgtc
      661 agttcgtggc agaacgacct gaccagctgt gggtggctga ttttacttac gtcagcacat
      721 ggcagggctt cgtctatgtg gcgtttatca ttgatgtgtt tgccggatac atcgtggggt
      781 ggcgggtctc atcgtctatg gaaacgacat tcgtgctgga tgcgctggag caggcgttgt
      841 gggcccgtcg tccgtctggc accatccatc acagcgataa aggctctcag tatgtgtcac
      901 tggcctatac ggagcgacta aaagaagccg gattactggc atcaacaggg agtacaggcg
      961 actcgtatga caacgcgatg gctgagagca tcaatggtct ttacaaagcg gaggtaatac
     1021 accgtaagag ctggaaaaac cgtgcagaag tggaactgg
//
LOCUS       DAZFTN010000383         1029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFTN010000383 DAZFTN010000000
VERSION     DAZFTN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..344
                     /locus_tag="ACHKV2_005533"
     CDS             <1..344
                     /locus_tag="ACHKV2_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6943182.1"
                     /translation="AGPAGPAGPQGPKGETGVAGPVGAAGPQGPKGDPGETQIRFRLG
                     SGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDVKGMD
                     EVGSDTGRTGE"
     gene            346..615
                     /locus_tag="ACHKV2_005534"
     CDS             346..615
                     /locus_tag="ACHKV2_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6943183.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            728..>1029
                     /locus_tag="ACHKV2_005535"
     CDS             728..>1029
                     /locus_tag="ACHKV2_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO6943184.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQ"
BASE COUNT          269 a          203 c          318 g          239 t
ORIGIN      
        1 cggcaggccc ggcaggcccg gcaggcccac agggaccgaa aggagaaaca ggtgtggctg
       61 gcccggtagg ggcagccgga cctcaggggc cgaagggcga cccgggagag acgcaaatac
      121 ggttccgtct ggggtcggga aacattattg agacaaacag caatggctgg ttcccggata
      181 cagatggcgc actcatcacc ggactgacct ttcttgaccc caaagatgcc acacgggttc
      241 aggggttttt tcagcatttg caggtcagat ttggcgacgg gccgtggcag gatgtcaagg
      301 ggatggatga agtgggcagt gatacaggca gaacaggaga atgacatgaa catactaaaa
      361 aaacttatgc agcgtctgtg tggttgcgga aagcatgatg accgtgaaca cggggagtta
      421 cttacagcac aactgcgact ggggccggca gacatcctgg agtcagatga gaatggcatt
      481 attccggagc aggacagggt aatcacacag gtggtgatac tggatgcgga taaaaagcag
      541 atacagtgcg tggtaagacc gctgcaaatc ctacgtgctg acgggaggtg ggaaaatatt
      601 ggcgggatga aatagccgac gggttcacaa aaaccggagt cctgctctgg tttttgttgt
      661 cacgtatggg ggatgtttgt tacagctatt taagtctgga gttcaaatta aaatagggag
      721 ctttgttatg ccattaacct cagatattag atcacattca tttaatcttg gggtggaggt
      781 tgttcgtgcc cgaattgtag ccaatgggcg cggagatatt acagtcggtg gtgaaactgt
      841 cagtattgtg tatgattcta ctaatgggcg cttttcatcc agtggcggta atggcggatt
      901 gctttctgag ttattgcttt tgggatttaa tagtggtcct cgagcccttg gtgagagaat
      961 gctaagtatg ctttcggact caggtgaagc acaatcgcaa gagagtattc agaacaaaat
     1021 atctcaatg
//
LOCUS       DAZFTN010000384         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFTN010000384 DAZFTN010000000
VERSION     DAZFTN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(185..985)
                     /locus_tag="ACHKV2_005536"
     CDS             complement(185..985)
                     /locus_tag="ACHKV2_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943185.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          287 a          222 c          185 g          330 t
ORIGIN      
        1 tagcaattct ttccattgca gatgatacta ttttaattta tgaagaatta aaaaaataac
       61 tcacatgcaa tacactggta atatatatta taactttata aaaacaatca tatttacatt
      121 atagaaatac tcaactcctg tttgctgcca ccctaaacta caggcggcag cataacaata
      181 acgattactg gaatcgactc accaagtctt tcaccatcga tacatatgtt ttttgtttca
      241 aattaaacag ttgcgcttcc gtaacatggt catttgatag ttctttattg ctcgtttttg
      301 ttattccagc cggcggaaaa attactctcg attcataatt caccggcgac caggtagccc
      361 actcacctgt tgccacatcc accagcgtga aacgtaccag gtagcgcagg gagatagcgt
      421 cagtgagttt ttcattttta tagtcggacc atactgttga tttcagcgca gagtcatatt
      481 ttcctgtctg caacatatcc tggtaaacaa taatcgcttt ctgatgtcct tttgcagcca
      541 caaaacgcag tgcctgcatc cagttcatat tctcagcacg ggcaacatcc tcgttctcat
      601 ttttatcttt gtttttgtta caagtcagag gcttttgcct gtccgggata ccagagaatg
      661 tggaaacagt ataatatttt cgcatctcct cctgcataat ggtttccggt gcgcggttgc
      721 cggattgaac aagtattaca ggagaattaa ggggaacgcg gaactcatct ccatcaagcg
      781 ctgcttgaat atcctcttcg gatacggcca gagtggtttc attaccgaat aaatctttgt
      841 ctgtcagttg ctggggagtg tttttattaa tattactttg tgtgacttga gcttttttct
      901 tcttttcatc aatagaagat tcagaggcac atcctgcaag cagcgagaat aacacacata
      961 tgcaacaaag cttttttgaa ttcataattg gacactccct cgcctgtcat aatgattaga
     1021 atat
//
LOCUS       DAZFTN010000385         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFTN010000385 DAZFTN010000000
VERSION     DAZFTN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(49..498)
                     /gene="sctT"
                     /locus_tag="ACHKV2_005537"
                     /pseudo
     CDS             complement(49..498)
                     /gene="sctT"
                     /locus_tag="ACHKV2_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(500..760)
                     /locus_tag="ACHKV2_005538"
     CDS             complement(500..760)
                     /locus_tag="ACHKV2_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6943186.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(771..>1018)
                     /locus_tag="ACHKV2_005539"
     CDS             complement(771..>1018)
                     /locus_tag="ACHKV2_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6943187.1"
                     /translation="LLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMS
                     PVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          360 a          219 c          169 g          270 t
ORIGIN      
        1 tttaggaaaa ttaaaacttc ctgaaggcca taaattataa ctttgctaaa gagtttccag
       61 aataaagttc aatccaccgt tagtaagata aaccgctgca gagaaaaggt taaataatct
      121 tgctaattcc gaggtatcga cacctgtagc cggatccaaa gtactactta acgttgcacc
      181 gcgttggtta tcgataaagc tacctatagc aaggaaaatc caaaatggag atgctaataa
      241 gcaagcaatg aacagcccta tgataatttc tcttgcagct aacaaagcga tatcgatttc
      301 atttaaggtc gataattcat agtggtaatg ctgaactaat gcacatgata ccactattat
      361 tattggatga cggactactg ttggtatatt cccactggcg aaaaatggta acaaatagaa
      421 agttggtgcc aatcggcaaa aaccgagcgc tgtagcagcc agtagactat gcaactgata
      481 gagtatcacc tcccccatgc tatcctctcg caaatgctaa attcagcatt tcaataccaa
      541 aagaatataa tttttcacca taccatccgc tcattaaaaa aaagcaaata cttacgcaca
      601 ataatttcac gccaaatggc aacgtctgtt cttgcaactg cgttaccgtt tgaaaaagcc
      661 ctaccaataa accaacaaat gtcgctactg caataggtcc tgcagacata actaaaatca
      721 gatacagcgc cctattccct gcaaaaacaa tgtcatccat atttttttcc tcagggatta
      781 atggataggt caaaatattg taatatgagt ccttttgata acattgtcca accgtccatc
      841 gcgacaaaca gaattaattt tatcggtgta gatatggtta ccggactcat catcatcata
      901 cctaacgtta acagaacgct ggatataacc agatccacta caacaaaggg taaatagata
      961 taaaaaccta taataaatgc actttttatc tcacttaacg catatgccgg gagtaacg
//
LOCUS       DAZFTN010000386         1017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFTN010000386 DAZFTN010000000
VERSION     DAZFTN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(112..801)
                     /locus_tag="ACHKV2_005540"
     CDS             complement(112..801)
                     /locus_tag="ACHKV2_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6943188.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="ACHKV2_005541"
     CDS             complement(816..938)
                     /locus_tag="ACHKV2_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6943189.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          267 a          260 c          194 g          296 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aactacctgt aattgccccc cctttttttg taacagt
//
LOCUS       DAZFTN010000387         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFTN010000387 DAZFTN010000000
VERSION     DAZFTN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            264..866
                     /gene="fimB"
                     /locus_tag="ACHKV2_005542"
     CDS             264..866
                     /gene="fimB"
                     /locus_tag="ACHKV2_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO6943190.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          308 a          176 c          208 g          315 t
ORIGIN      
        1 cattaatgat gagggttatt attagattcg tatccgattg ataaatatat aaaggtacat
       61 agcatgcaag agcatagcgt ttgtatggca acgttattat aattaactgt tgctactcca
      121 tttaagttca ctcagaagaa ctggtccact tacgttagtt attaagcaaa cgttcgcttt
      181 tataaacata atcaggataa aaatgttgga ttattgctaa cctagcacag ctagtgcgcg
      241 tctgtaatta taagggaaaa acgatgaaga ataaggctga taacaaaaaa aggaacttcc
      301 tgacccatag tgaaatcgaa tcactcctta aagcagcaaa taccgggcct catgcagcac
      361 gtaattattg tctgactttg ctttgtttta ttcatggttt ccgggcgagt gaaatttgtc
      421 gattgaggat ttcggatatt gatcttaagg caaagtgtat atatatccat cgattaaaaa
      481 aaggcttttc aacaacgcac ccgctattga acaaagaagt tcaggcttta aaaaactggt
      541 tgagtatccg tacttcttat ccgcatgctg agagcgagtg ggtattttta tcgcgtaagg
      601 ggaatccgct ttctcggcaa caattttacc atattatctc gacttccggt ggtaatgccg
      661 ggttgtcact ggagattcat ccgcacatgt tacgccattc gtgtggtttt gctttggcga
      721 atatgggaat agatacgcga cttatccagg attatcttgg gcatcgcaat attcgtcata
      781 ctgtctggta tactgccagc aatgcagggc gtttttacgg catctgggat agagccagag
      841 gacgacagcg tcacgctgtt ttatagagat agttgagata ccagggatgg tgttaaaaac
      901 tacattattc ttacgatata tcaaaaatga ttaaccattt attaattgat gttattgtaa
      961 gtatgattct taattatcat aataaacatt aagttaacca tatccat
//
LOCUS       DAZFTN010000388         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFTN010000388 DAZFTN010000000
VERSION     DAZFTN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..568)
                     /locus_tag="ACHKV2_005543"
     CDS             complement(8..568)
                     /locus_tag="ACHKV2_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6943191.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(582..761)
                     /locus_tag="ACHKV2_005544"
     CDS             complement(582..761)
                     /locus_tag="ACHKV2_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943192.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISALKGLSTSF"
     gene            790..993
                     /locus_tag="ACHKV2_005545"
     CDS             790..993
                     /locus_tag="ACHKV2_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943193.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
BASE COUNT          252 a          250 c          235 g          270 t
ORIGIN      
        1 gagcgtctta tttttccgaa acgctcgcag gataataacg ataaagggtt gaaaggccga
       61 tatcaaaaat aagtgccacc tgtcttcgac tgacaccccc ggccagtaaa cgcccgacct
      121 gctcgtattg ttcctgcgtg agacgacggg gtctgccgcc ggttcgtccc ctttcacggg
      181 cagctgcaag accagcccgt gtccgttcga caattaattc acgctccatt tctgccagcg
      241 cacccattat gtgaaaaaag aagcgcccca tgggggttga cgtatcaatg ttgtcagtaa
      301 gactacgaaa atttatcccc tgttgctgta attcatctgt cagcgcaaca aggtggcgca
      361 tactgcgccc cagcctgtcc agtttccaga ccatcagtgt gtcaccgggt tttagtttct
      421 tcagtgcccg cttcagtccc ggccgctgtg tcgttttacc actcatttta tcttcaaaaa
      481 tctgctcaca tcctgcacat tccagcgcat ttctttgtaa acctgtgttc tggtcatttg
      541 ttgatacgcg tatatagcca atcagcatgt atttaccttc cttaaaaaga ggtggaaagg
      601 ccttttaaag cagatatacg gcatccctga aacctcggtt tacgggatac tgtaaacagg
      661 gccgcgaatg ccctgccatc gaacggagcg gctgttgcag cgaataaact cgccactcca
      721 agaaatatta atggtgtccc atttgacgga acgcaggata taaatattac atcggggatg
      781 acgcagtctg tggctgactc acgttatgtt caaagcattc gaagaggttc aaggtcaacg
      841 attggaatgc aatataatat ttttgaggtt cctgacggat gcgtacttac agggcttgat
      901 gtcgctggtg atggtaatgc aacggtaacc gcatattatc gacctgttca gtttctgata
      961 gatggttcgt ggaaaacagc ttccagcgca taaaagtgca gtagttc
//
LOCUS       DAZFTN010000389         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFTN010000389 DAZFTN010000000
VERSION     DAZFTN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1007)
                     /gene="nleL"
                     /locus_tag="ACHKV2_005546"
     CDS             complement(<1..>1007)
                     /gene="nleL"
                     /locus_tag="ACHKV2_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO6943194.1"
                     /translation="NEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGGCYIDL
                     SDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRPY
                     VKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMY
                     FERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCD
                     GSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGE
                     VQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKEKFIEALNSNK
                     S"
BASE COUNT          350 a          203 c          140 g          314 t
ORIGIN      
        1 tctgatttat tggaattaag tgcctcaata aatttctctt tgagttcatt atcagaaatg
       61 agtatatcca gaataccaga aacgaaattc tgggaaaaag ctttatgata aggaatactg
      121 aaaattggaa atgattcaga aaaaagattg ctcaatgaat acccaaatgt attttgaact
      181 tctccatcct tatataaaaa aaaggacatc caattagttg atgtattagg agataacata
      241 tcaaccatat gactcaaaga acacatcatt gccataccat catcctgtga tgataacaaa
      301 acccagttat aagccaaatc ggaatcatca tcccagtcag gcttcccaga gccatcgcac
      361 aatccaaaat cactctgctg tgtgacagga taaacttttt cattttttaa atatacttca
      421 tagagttccc taaatcgttc ctgcattcca tcacgccgtg aaccattcat tacaatttgt
      481 ataaaggaac tattataaga taccatcgca cctggatgtc gctcaaaata catccctgcc
      541 tgcaataaac taccattctc catatacatc attattgaat tattaaacca agacattata
      601 gtatcaaaaa agtcatcaca tataggctcg agccattgct gtatatttgc gttgttcaaa
      661 taaggatgaa tactaaatga atcagccaaa gacatcttaa catagggccg gaacgccgct
      721 aattcgtcaa gagatatttt tttgataagt tcttcagcgg cccttacttt atcatggtta
      781 tacttatcat cagatgtatt catagtcaat aaaattgatt tatttgattt tgtatagtta
      841 tccaacacag ataatatatt ttcctgtatg tcagatagat ctatgtaaca ccctcctaga
      901 ttaatggtat tacttttttc actaaagagc tcctttacat cacctaagat agcaccactt
      961 aaaaccattc caggaattat aatagctcct gttaaatcag cctcgtt
//
LOCUS       DAZFTN010000390         1001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFTN010000390 DAZFTN010000000
VERSION     DAZFTN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..613
                     /gene="wzx"
                     /locus_tag="ACHKV2_005547"
     CDS             <1..613
                     /gene="wzx"
                     /locus_tag="ACHKV2_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO6943195.1"
                     /translation="FFFFILQLGTLATWSGDNFIISITLGVTYVAVFSITQRLFQIST
                     VPLTIYNIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVVNI
                     WTEGKIQVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILIAIPAKY
                     IIVSHFGLTVMLYCFIFIYIVNYFIWYKCSFKKHIDRQLNIRG"
     gene            616..>1001
                     /gene="rfbE"
                     /locus_tag="ACHKV2_005548"
                     /gene_synonym="perA"
     CDS             616..>1001
                     /gene="rfbE"
                     /locus_tag="ACHKV2_005548"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFO6943196.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNETWQMSVSDIEQKITNKTKAIMCV"
BASE COUNT          335 a          149 c          176 g          341 t
ORIGIN      
        1 ttttttcttt tttattttac agttaggcac tctggcaaca tggagtggtg ataactttat
       61 aatatctata acattgggtg ttacttatgt tgctgttttt agcattacac agagattatt
      121 tcaaatatct acggtccctc ttacgattta taacatcccg ttatgggctg cttatgcaga
      181 tgctcatgca cgcaatgata ctcaatttat aaaaaagacg ctcagaacat cattgaaaat
      241 agtgggtatt tcatcattct tattggcctt catattagta gtgttcggta gtgaagtcgt
      301 taatatttgg acagaaggaa agattcaggt acctcgaaca ttcataatag cttatgcttt
      361 atggtctgtt attgatgctt tttcgaatac atttgcaagc tttttaaatg gtttgaacat
      421 agttaaacaa caaatgcttg ctgttgtaac attgatattg atcgcaattc cagcaaaata
      481 catcatagtt agccattttg ggttaactgt tatgttgtac tgcttcattt ttatatatat
      541 tgtaaattac tttatatggt ataaatgtag ttttaaaaaa catatcgata gacagttaaa
      601 tataagagga tgaaaatgaa atatatacca gtttaccaac cgtcattgac aggaaaagaa
      661 aaagaatatg taaatgaatg tctggactca acgtggattt catcaaaagg aaactatatt
      721 cagaagtttg aaaataaatt tgcggaacaa aaccatgtgc aatatgcaac tactgtaagt
      781 aatggaacgg ttgctcttca tttagctttg ttagcgttag gtatatcgga aggagatgaa
      841 gttattgttc caacactgac atatatagca tcagttaatg ctataaaata cacaggagcc
      901 acccccattt tcgttgattc agataatgaa acttggcaaa tgtctgttag tgacatagaa
      961 caaaaaatca ctaataaaac taaagctatt atgtgtgtcc a
//
LOCUS       DAZFTN010000391          993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFTN010000391 DAZFTN010000000
VERSION     DAZFTN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHKV2_005549"
     CDS             complement(<1..203)
                     /locus_tag="ACHKV2_005549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943197.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
     gene            complement(361..>993)
                     /locus_tag="ACHKV2_005550"
     CDS             complement(361..>993)
                     /locus_tag="ACHKV2_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6943198.1"
                     /translation="LGETEVVSNLIKLGVDLDARGDLGRTALNEAVSNGYFDIVKLLV
                     ESGADLTIKDEFGKTALELAEVHQNYKIVEWLSHYRDHNPIPDFSVLINIYGERYFGG
                     EIIDTDARTELGEGVLHIAVRKRRQLDVRCFIQHGADINAKANNGFDFTPLHYSIGIG
                     DFDMMKLLLKLGADIYLESEMGYSPMDLAILMGDKRIIFYLYGYISHVSE"
BASE COUNT          291 a          252 c          157 g          293 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agcgtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 ttattcagaa acatgagaaa tataaccata taaataaaaa ataattctct tatcccccat
      421 caaaatagcc aaatccatag gggagtatcc catctcactc tccaaataaa tatcagcacc
      481 taatttcaac aaaagcttca tcatatcaaa atcccctatc cctatcgaat aatgcaaagg
      541 agtgaagtca aagccattat ttgccttagc atttatatcg gcaccatgtt gaataaaaca
      601 tctgacatct aattgtcttc ttttcctaac agctatatga agaaccccct cgccaagttc
      661 tgtccttgca tctgtatcta taatctctcc accaaaatat cgctcaccgt agatattaat
      721 aagtacagaa aaatcgggta tgggattatg atctctataa tgtgatagcc attcaactat
      781 tttataattc tggtgtacct cagccaattc taatgccgtt ttcccaaatt catcctttat
      841 agtaagatcg gctcctgatt ctactaaaag cttaacaata tcaaaatacc cattacttac
      901 ggcctcatta agtgcggtac gccccaaatc cccacgagca tccagatcca cacccaattt
      961 aattaaatta cttacaacct cagtctcacc taa
//
LOCUS       DAZFTN010000392          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFTN010000392 DAZFTN010000000
VERSION     DAZFTN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..582
                     /locus_tag="ACHKV2_005551"
     CDS             <1..582
                     /locus_tag="ACHKV2_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6943199.1"
                     /translation="NELNEKGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSN
                     LFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMS
                     ELTAMTASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTD
                     IRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            593..>992
                     /locus_tag="ACHKV2_005552"
     CDS             593..>992
                     /locus_tag="ACHKV2_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6943200.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRLFEHGVTDCYTLFRDAYHLAG"
BASE COUNT          193 a          236 c          352 g          211 t
ORIGIN      
        1 aatgagctga atgaaaaagg ggaggcggtc acctggcagg ggcggcaata tcaggtatac
       61 ccgattgacg gcagcggttt tgagatgaac gggaagggca gcagtgccag accgtcgctg
      121 acggtgtcca atctgtttgg tctggtcacc gggatggcgg aggacctgca gagcctggtg
      181 ggggccacgg tggtccgccg ccgggtgtat gcccgttttc tggatgcggt gaattttgtg
      241 gcgggcaatc cggaagcgga cccggagcag gagctgagcg accgctgggt ggtggagcag
      301 atgtcagagc tgacggccat gacagcctcg tttgtgctgg ccacaccgac ggagacggac
      361 ggagcgctgt ttcccggtcg cattatgctg gcgaatacct gtatgtggac ctaccgctct
      421 gatgagtgtg gttacacggg cggggctgtg gcggatgagt tcgataaacc caccaccgat
      481 atccgtaagg acagatgcag caagtgcatg cgcgggtgtg agatgcgcgg catggtggct
      541 aattttggcg gtttcctttc cattaataaa ctttcgcagt aaatcccggt ttatgacaca
      601 gactgaatca gcgattctgg cgcatgcccg gcggtgtgcg cctgcggagt cgtgcggctt
      661 cgtggtgaga acgccggagg gggagcggta tatcccttgt gtgaatatct ctgcagagcc
      721 ggaggcgtat tttcgtattg caccggaaga ctggctgcgg gcagagatgc agggggagat
      781 tgtggcactg gtccacagtc atcccggtgg tctgccctgg ctgagcgagg ccgaccggcg
      841 gctgcagata aaaagtgcac tgtcctggtg gctggtctgc cggggggaaa ttcataaatt
      901 ccgctgtgtg ccacatctga caggacggct ctttgagcac ggggtgacgg actgttacac
      961 gctgttccgg gatgcatacc atctggcggg aa
//
LOCUS       DAZFTN010000393          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFTN010000393 DAZFTN010000000
VERSION     DAZFTN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..582
                     /locus_tag="ACHKV2_005553"
     CDS             <1..582
                     /locus_tag="ACHKV2_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6943201.1"
                     /translation="NELNEKGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSN
                     LFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMS
                     SLTAMTASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDNPTTD
                     IRKDRCSKCMRGCEMRSMVANFGGFLSINKLSQ"
     gene            593..>992
                     /locus_tag="ACHKV2_005554"
     CDS             593..>992
                     /locus_tag="ACHKV2_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6943202.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAG"
BASE COUNT          191 a          237 c          352 g          212 t
ORIGIN      
        1 aatgagctga atgaaaaagg ggaggcggtt acctggcagg ggcggcaata tcaggcatac
       61 ccgattgacg gcagtggctt tgagatgaac gggaagggca gcagtgccag accgtcgctg
      121 acggtgtcga atctgtttgg tctggtcacc gggatggcgg aggacctgca gagcctggtg
      181 ggggccacgg tggtccgccg ccgggtgtat gcccgttttc tggatgcggt gaattttgtg
      241 gcaggcaatc ctgaggcaga ccctgagcag gagctgacgg accggtgggt ggtggagcag
      301 atgtcatcgc tgacggccat gacggcctcg tttgtgctgg cgacaccgac ggagacggac
      361 ggagcgctgt ttcccggtcg cattatgctg gcgaatacct gtatgtggac ctaccgctct
      421 gatgagtgtg gttacacggg cggggcagtg gcggatgagt tcgacaaccc caccacggat
      481 atccgtaagg acagatgcag caagtgcatg cgcgggtgtg agatgcgcag catggtggct
      541 aattttggcg gtttcctttc cattaataaa ctttcgcagt aaatcccggt ttatgacaca
      601 gactgaatca gcgattctgg cgcatgcccg gcggtgtgcg cctgcggagt cgtgcggctt
      661 cgtgataagc acgccggagg gggagtggta tatcccttgt gtgaatattt ctgcagagcc
      721 ggaggcgtat tttcgtatcg caccggaaga ctggctgcgg gcagagatgc agggggagat
      781 tgtggcactg gtccacagtc atcccggtgg gctgccctgg ctgagcgagg ctgaccggcg
      841 gctgcagata aaaagcgcac tgccctggtg gctggtctgc cggggtgaca ttcacaaatt
      901 ccgctgtgtg ccacatctga caggacggcg ctttgagcac ggggtgacgg actgttacac
      961 gctgttccgg gatgcttatc atctggcggg ga
//
LOCUS       DAZFTN010000394          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFTN010000394 DAZFTN010000000
VERSION     DAZFTN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..69
                     /locus_tag="ACHKV2_005555"
     CDS             <1..69
                     /locus_tag="ACHKV2_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943203.1"
                     /translation="EWSVTDNARYSDFSCTIEQVVN"
     gene            69..431
                     /locus_tag="ACHKV2_005556"
                     /pseudo
     CDS             69..431
                     /locus_tag="ACHKV2_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            694..>986
                     /locus_tag="ACHKV2_005557"
     CDS             694..>986
                     /locus_tag="ACHKV2_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943204.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKA"
BASE COUNT          249 a          198 c          332 g          207 t
ORIGIN      
        1 gagtggagcg tcacggataa cgcccggtac agtgatttca gctgtacgat tgagcaggtg
       61 gtgaactgat gcaggacatt catgaagaaa gtctgaacga gtcggttaaa tcagagcagt
      121 caccgcgggt ggtgctctgg gaaattgacc tgacggtgca gggcggtgag cggtattttt
      181 tctgtaatga gctgaatgaa aaaggggagg cggtcacctg gcaggggcgg gaatatcagg
      241 catacccgat tgacggcagc ggttttgaga tgaacgggaa gggcagcagt gcccgcccgt
      301 cactgacggt gtccaatctg ttcggtctgg tcaccggaat ggcggaggac ctgcagagcc
      361 tggtgggggc cacggtggtc cgccgccggg tgtatgcccg ttttctggat gcggtgaatt
      421 ttgtggcggg cggtcgtggt tatcgggcgg cagaggtaaa aagaataaaa aaatcccgca
      481 gagttgcggg gacagacaaa gattaacgtt aaggagttat ttttgtttct ggtgctcggg
      541 caaaaaaaca ttaacgcaga gaaattatta gcaccacagt cagtttgtga aaatgtgaag
      601 atattcagaa gttttattca gtcatgatac aggcatcctc cgggatgcct gttgtttttg
      661 tgcgtaacag ttatcacagt aaagggtgag acaatgggca aaggtggcgg caaggcgcac
      721 acgccggtag aggcaaagga caatcttaag tccacgcaga tgatgagcgt gattgatgcc
      781 attggtgaag ggccgattga aggtccggtg aaggggctgc agagtatcct ggtgaacaaa
      841 accccgctga cggacacgga cggtaatcct gtgatacatg gtgtgacagc ggtctggcgc
      901 gccggggagc aggagcagac accacctgaa ggctttgagt cctccggggc ggaaaccgca
      961 ctgggcgtgg aagtgacgaa ggcaaa
//
LOCUS       DAZFTN010000395          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFTN010000395 DAZFTN010000000
VERSION     DAZFTN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..650
                     /locus_tag="ACHKV2_005558"
     CDS             <1..650
                     /locus_tag="ACHKV2_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6943205.1"
                     /translation="TKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNK
                     SDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMG
                     LITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEP
                     RRRKAVVIKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLKQLTRICMR"
     gene            817..>974
                     /gene="ygeI"
                     /locus_tag="ACHKV2_005559"
     CDS             817..>974
                     /gene="ygeI"
                     /locus_tag="ACHKV2_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO6943206.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDKVQIQAF
                     IVSTWMAS"
BASE COUNT          366 a          155 c          173 g          280 t
ORIGIN      
        1 taactaagca ggaccaagga tataagaatc attatttatc agatgaaatg ttagccggaa
       61 agaaagaact ttacgagttc acccctgaaa gcatttacag ggccatgact atatttgatg
      121 gattacaaaa taaaagtgat atacagacgc taaaaacaga atgttattgc cttctagcgg
      181 aatgccatat gtctttggca cttcatggaa aaagtgaact tgaacttgct gctcaaaaag
      241 cattagagct tttagattat gtatcagaca taaccactgt cgatggaaaa attttagcta
      301 ttatgggact gataactggt ctgtctggac aagcaaaagt atctcatatc ttatttgaac
      361 aggctaagat acactcaact gatatagcct ctctctacta ctatagggca cttgtcaact
      421 ttcataatga aaaaattgaa gaggcaagga tttgtataga caaatcacta caactcgaac
      481 ccagaagacg aaaagcagtt gtgataaaag aatgtgtaga tatgtatgtg cctaacccgc
      541 tcaaaaaaaa catgaaactc tactataaag aaactgagag tggaagccat cgagttataa
      601 ttgacaacat tttgaaatta aagcagctga cgagaatttg tatgcgataa atttttattg
      661 acacaatttt attatttaga tgtatgcaac ttgttattta aaaaacaaag atcaaaatat
      721 ctaagatatt taccttattg caagatattt aaaatgctct agagttaaaa gtatgatgta
      781 aagaccattg gcctggacaa ttaaaaaggg agctttatga ctaatccaat cggtatcaat
      841 aatttatctc aaagttcaaa tatagcgaat gcaacgggtg atgaagtcgt ttcattagat
      901 aaacacatca acaccagtgc tactgataaa gttcaaatac aagcatttat agtttcaaca
      961 tggatggcgt cttt
//
LOCUS       DAZFTN010000396          965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFTN010000396 DAZFTN010000000
VERSION     DAZFTN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..413)
                     /locus_tag="ACHKV2_005560"
     CDS             complement(<1..413)
                     /locus_tag="ACHKV2_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO6943207.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARP"
     gene            complement(397..>965)
                     /locus_tag="ACHKV2_005561"
     CDS             complement(397..>965)
                     /locus_tag="ACHKV2_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HFO6943208.1"
                     /translation="YRTLSSLRYVLDEQDNPQTLIGLTPAAQKNIRLIRAVMQSAAVV
                     AQAQTVGKLLDQVLSQETLPDSDAAHRTWPVWLESSVDLQRINRDLSEALERQVMTLS
                     GQGYTATALTLRDARLALTEDLNTRGVQLPGATVVTVRTTEPALVTLYRATGNSTGWQ
                     RFVRRNGIVDPLFIPGGHSVEVISEQQG"
BASE COUNT          216 a          299 c          258 g          192 t
ORIGIN      
        1 aagggccggg ctgccgttgc atcatttacc tgccagcgca ccgtgacccc gaacggagca
       61 cacaaatctg aagcaatgtg ctccagcgtg cggttcctcc actggctgcc cggatgaatg
      121 gctgaacagt ccaccaggtc accggtttta tcccgtccgc tgagcgtgat ctgaaaacgc
      181 gtggcgctga tgcgctgccg gacctgatcc agccagccag tgatcacagg ctgaccgtca
      241 atgcgcaatt ccagagactg accggcacgg acggatgacg gaaggcgtac accgggcatc
      301 attaccccca gttcaaagga gcccgccaga tgttcaagag agcgacgaac actcaccgtc
      361 agccagccgg aaaaaatctc accgcccaga tacagttcaa ccctgctgct cactaatcac
      421 ctccactgaa tggcctccgg gaatgaacag cggatcaaca ataccgttac ggcgcacaaa
      481 acgttgccag ccggtactgt tcccggtggc acggtacagg gtcaccagtg caggctcggt
      541 ggtacgtaca gtcactactg ttgcaccggg tagctgaact ccccgtgtat tcagatcctc
      601 tgtcagtgcc agtctggcat cacgaagcgt cagcgccgtg gcggtatacc cctgcccgga
      661 cagtgtcatc acctgccgtt cgagcgcttc gcttaagtca cggttaatgc gctgaagatc
      721 aactgaactt tccagccaga caggccaggt acggtgggct gcgtcactgt ccggcagggt
      781 ttcctgactg agaacctgat ccagcagttt tcccacggtc tgtgcctggg ccaccacggc
      841 agcactctgc attaccgccc gtatcagacg aatattcttt tgtgctgccg gtgtcagacc
      901 gatgagagtc tgcggattat cctgctcatc cagcacgtaa cgcagggaag ataatgtcct
      961 gtaca
//
LOCUS       DAZFTN010000397          958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFTN010000397 DAZFTN010000000
VERSION     DAZFTN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..100
                     /locus_tag="ACHKV2_005562"
     CDS             <1..100
                     /locus_tag="ACHKV2_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6943209.1"
                     /translation="RIPTASQNRSPAPVFVVMSGEYLLDKKEEIIQ"
     gene            113..202
                     /locus_tag="ACHKV2_005563"
     CDS             113..202
                     /locus_tag="ACHKV2_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943210.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            222..>958
                     /gene="nleL"
                     /locus_tag="ACHKV2_005564"
     CDS             222..>958
                     /gene="nleL"
                     /locus_tag="ACHKV2_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO6943211.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLEN"
BASE COUNT          285 a          161 c          210 g          302 t
ORIGIN      
        1 acgtattccg acagcttcac aaaaccggag tccggctccg gtttttgttg tcatgtccgg
       61 ggaatatttg ttagataaaa aagaggagat aattcaatag ggagttaaat taatgccgat
      121 aaatctgaca tcttatttgt gggtacaggg acagaaagtt gtcccggcag ttgtgttttc
      181 taattttact ttagtggact aagtaaaaag gagtgagata aatgctgccc actacaaata
      241 tctctgtaaa ttctggagta atatcttttg aaagtcctgt agattcacca tctaacgagg
      301 atgttgaagt tgccctcgaa aagtggtgtg ctgagggaga atttagcgaa aatcgtcatg
      361 aggttgcatc aaaaatactt gatgttataa gtactaatgg agagacttta tcaatcagtg
      421 agccaataac aacattacca gacttgcttc caggttctct gaaagaactg gttttgaatg
      481 gatgtacaga gcttaaatca ataaactgct taccccccaa cttatcttca ttaagtatgg
      541 ttggatgctc atcattagag gttataaatt gcagcatacc tgaaaatgtc attaatttat
      601 ctttatgcca ttgtagttct ttgaaacata tagaaggttc ctttcctgag gcactcagaa
      661 attccgtata tttaaatggc tgtaattcat taaatgaatc gcaatgtcaa ttccttgcat
      721 atgatgtcag tcaaggccgt gcctgcctga gcaaagctga gcttactgct gacttaattt
      781 ggttgtcagc taaccgaacg ggtgaagagt ctgctgaaga attgaattac tctggatgtg
      841 acttgtcagg tctaagtctt gtagggctga atttatcatc agtaaatttt tctggagcag
      901 tgcttgatga tacagatctc aggatgagtg atttgtctca ggctgtattg gaaaactg
//
LOCUS       DAZFTN010000398          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFTN010000398 DAZFTN010000000
VERSION     DAZFTN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..132
                     /locus_tag="ACHKV2_005565"
     CDS             <1..132
                     /locus_tag="ACHKV2_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6943212.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            83..784
                     /gene="traT"
                     /locus_tag="ACHKV2_005566"
     CDS             83..784
                     /gene="traT"
                     /locus_tag="ACHKV2_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO6943213.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(832..>956)
                     /locus_tag="ACHKV2_005567"
     CDS             complement(832..>956)
                     /locus_tag="ACHKV2_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6943214.1"
                     /translation="YSYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
BASE COUNT          276 a          233 c          228 g          219 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      181 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      241 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      301 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
      361 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
      421 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
      481 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
      541 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
      601 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaaac
      661 cggcaaccag cataaatacc agacccgggt tgtatccagt gccaaccagg ttaatctgaa
      721 gtttgaagaa gctaaacctc atctggaaga ccagctggca aaatcaatcg ccaatatact
      781 ctgatataaa tatcccggtg gccattcacc tggcctccgg ccctcttagt ctcaacgttc
      841 agacaattca gatattattt tccctgtcac accatatacc catgccggaa cagaatctaa
      901 tgacggtacc agtaaattct tcagcgttcg gtttgcctga cgcccgtagc tgtatt
//
LOCUS       DAZFTN010000399          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFTN010000399 DAZFTN010000000
VERSION     DAZFTN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>955
                     /locus_tag="ACHKV2_005568"
     CDS             <1..>955
                     /locus_tag="ACHKV2_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6943215.1"
                     /translation="TITYEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVES
                     LDRLSREKVGEAIERLKLILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANE
                     ESEIKSSRVKLSWKKKRQDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIF
                     KLRMERRSLNAIAKYLNDHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGK
                     GISEIAGYYPRVISDDLFYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMI
                     VHAVSGSLHGYYVCPMRRLHRCDRPSIKRDLVDYNIINELLF"
BASE COUNT          316 a          142 c          216 g          281 t
ORIGIN      
        1 accataacat atgaagattt aggattaagt gcattcaaag gaaagcatgc acaatcagga
       61 gctttttcgg aatttttaga tgctatagag catggttata tattgccagg aactacattg
      121 ttagttgaaa gtctggacag actttcaaga gaaaaagtcg gtgaagcgat tgaacgtctg
      181 aaattgattt tgaatcacgg tattgatgtt ataactcttt gcgacaatac agtctataat
      241 attgactctt tgaatgagcc atattcatta ataaaagcca tacttatagc acaaagggca
      301 aatgaagaaa gcgagataaa gtcaagtcgg gttaaattat catggaagaa aaaacggcag
      361 gatgcactgg aatcaggtac gattatgacg gcgtcttgtc cgagatggct ctccttagat
      421 gacaaaagaa cggcttttgt tccagacccc gacagggtga aaactattga gctaattttt
      481 aaactcagga tggaaaggcg ctcattgaat gcaatagcca agtatttaaa tgatcatgct
      541 gtaaagaatt tctcaggaaa agaaagtgca tgggggcctt ctgtaattga aaaattatta
      601 gcgaataaag ctctgatagg tatatgcgta ccttcatatc gtgcaagagg gaaagggata
      661 agtgaaatcg ctggctatta tcccagagtc atatcagatg atttgtttta cgctgtacag
      721 gaaattcggt tggcaccttt tggtattagc aatagtagca agaatcctat gctaataaat
      781 ctacttcgaa cagttatgaa gtgtgaggct tgtggtaata ccatgattgt tcatgcggta
      841 tctggaagtt tgcatggcta ttatgtttgt ccgatgagaa gattacatcg atgtgacagg
      901 ccatcaataa agagagattt ggttgattat aatatcatta atgaattgct tttta
//
LOCUS       DAZFTN010000400          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFTN010000400 DAZFTN010000000
VERSION     DAZFTN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..>936
                     /locus_tag="ACHKV2_005569"
     CDS             71..>936
                     /locus_tag="ACHKV2_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6943216.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDI"
BASE COUNT          220 a          216 c          268 g          232 t
ORIGIN      
        1 ttaacgcaaa atggcgttta tcggtctgtg gatatgataa ttcagtttat tcaggaacag
       61 gctgattatt atgacgtcca aatgggttca actatcatca atgcccggaa actttactgt
      121 taaagtttcc ggtggtacag cggcatttct tgaggctccc tttcctccgg ccgaaacaaa
      181 aggaggaatg acatttgctg actgtctcat cagttttaat acacgggatt gcctgtgggt
      241 aaggccagta tccggtgatc cgagtgtgga aattaccgga gcgggtattg gtgcggtcat
      301 tccgttaagt gctgatgttg ccggtactgc tgaaccgtca gactgggata atgctgaaac
      361 acatacccgt ccgtcaggaa atgaaactgc ttccagctcc ccttcctggt attacgtggt
      421 ggttcttgct ggtcagtcaa acggtatggc ttacggtgaa ggtctgccac tgccggagac
      481 atatgaccgc cccgaaccgc gtattatgca gttagcccgt cgcagtacag tcacgccggg
      541 aggtaaagcc tgtcagtata acgacatcat tctggccgac cactgtctgc atgatgtgca
      601 ggatatgagt ggaaaaaatc acccgaaagc ggatgtggct aaagggcaat atggcaccgt
      661 gggtcagggg cttcacattg ccaaaaaact gctgccgttt attccggcag atgcggggat
      721 tctgctggtt ccctgctgcc gtggtggttc tgcattcacg gcgggtgctg acggcacgta
      781 cagtgacagc acgggtgctt cagaggactc agcccgctgg ggtgtggata agccgctgta
      841 taaggactta atcagccgga caaaagcggc actggcgaag aacccgaaaa accgcctgct
      901 tgctgtggtg tggatgcagg gcgagtttga tattga
//
LOCUS       DAZFTN010000401          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFTN010000401 DAZFTN010000000
VERSION     DAZFTN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..271
                     /locus_tag="ACHKV2_005570"
     CDS             71..271
                     /locus_tag="ACHKV2_005570"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO6943217.1"
                     /translation="MALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWKIEGKQPKNS
                     GGNFTMLIAKKDGQVLNVIHTK"
BASE COUNT          327 a          153 c          193 g          262 t
ORIGIN      
        1 attgtttaca atatcacact cagtatttgg aaaagaaaat ccagtggaaa tttaagtaaa
       61 tagtcgtgaa atggctttgg aattatcata tgtttacatt aaatatgtat acgggaaaga
      121 aaaagcagaa tttcagaaac catatagtat tacagatgac aataattgtt ggaaaatcga
      181 gggaaaacaa ccgaagaatt caggaggaaa ttttacaatg ctaatagcga aaaaagatgg
      241 gcaagtgttg aatgtaatac acacaaaata atccactgta cagtcgattc aaatatttta
      301 ccaaggtgtg tttcgtaaca attttgtgta caatatgact ctacatatcg acgaaaaaac
      361 cacttgattt gacaggaata cattcaaaaa atttcttttt tcatttcagg agcgattttc
      421 atctgttact tgaagtatct gtgagattaa gtgactcccc ctaacgtcgt actaaatagt
      481 aattactggc cataccggga gcagtattcg gcgctcccca catgcagcag aataaccgca
      541 tcagcgaaga tgtattttta ctatcacaat gtgtacggac ggtttatgga aaagaatgaa
      601 cattagatac gcgatgcggc ggcaggttac acggttatca ctacgatagc caatactggc
      661 tggtgcatta ccgccgtgaa caataggacg ccgtactgct ggaaatttct tacctttata
      721 actccccgcg ccgccgtatc gggaaacggg aagcaatcaa gatgaggctg atataatgtg
      781 cggaatgcct gagtattttt agaatagggg ggtaatatgt ttgatagaat atatcttggt
      841 gatcgagcaa taaaaaaatt gaatttgatt tatggagtaa acaaattaga attcaggtta
      901 atttaatctc aaggatagcc tatggtacaa cagaa
//
LOCUS       DAZFTN010000402          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFTN010000402 DAZFTN010000000
VERSION     DAZFTN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            280..587
                     /locus_tag="ACHKV2_005571"
                     /pseudo
     CDS             280..587
                     /locus_tag="ACHKV2_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(827..>916)
                     /locus_tag="ACHKV2_005572"
     CDS             complement(827..>916)
                     /locus_tag="ACHKV2_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO6943218.1"
                     /translation="PPPTGPSGLPPLAQALKDHLAAYEQSKKG"
BASE COUNT          283 a          205 c          176 g          252 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcgg
//
LOCUS       DAZFTN010000403          913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFTN010000403 DAZFTN010000000
VERSION     DAZFTN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..913
                     /locus_tag="ACHKV2_005573"
                     /pseudo
     CDS             1..913
                     /locus_tag="ACHKV2_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          276 a          169 c          275 g          193 t
ORIGIN      
        1 aggctggagc gggaactgcg gcagggcagc gtgagtgaag aaagccagca gtggcttgcg
       61 cagtgcgggc tgacggcgga acagatggcc gcgtagctgg aggcggaata tatcccggag
      121 cggaaacttc atctttacca ctgcgaccac cggggactgc cgcaggcgct catcagcccg
      181 gaaggggaaa cggcgtggca gggggagtat gacgagtggg gaaacctgct gggcgaaacc
      241 agcgcgcagc acctgcaaca gccgtaccgt ctgccgggac agcagtatga tgaggagtcg
      301 gggctgtatt acaaccgtca ccggtactat gacccgctac aggggaggta tatcacccag
      361 gatccaatag atataaaagg aggatggaat ttatattctt atgcgcttaa tccggtaagt
      421 tggattgacc cattaggatt gacgcagtgc gattccgaag ggtgtaataa tgatatatta
      481 tttacaggag gaagtggtcc cgataataaa atacttaatg aattaggtcc gagagatggc
      541 attgacggtt tgggttcaca aaacatgaaa atgtatagtg ggctattagg gggcgatatt
      601 ttaaaaccgg ggattctggg cggtttgacg cttggtagtg tgacacaacg accatcgcga
      661 acggctgagg aggaggtgca agctcaaata gaatatttag catataagaa gcgttgtgag
      721 caaaaacctg atagtaattt aagtcgttgt gctgccgcga tctttcaaaa gagcagaaaa
      781 gaagattgtt taagaatgag acaagaatgg gatgataaat ggtggcctgg caaacatgcg
      841 gatgaaataa aaaatgtgaa agcatcgttg aaaaacacac ttattgatgt taaaaaaaag
      901 atgcatgcca act
//
LOCUS       DAZFTN010000404          912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFTN010000404 DAZFTN010000000
VERSION     DAZFTN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          268 a          165 c          185 g          294 t
ORIGIN      
        1 ttcgtaagct atatcactta ctcaattcat tttacccaga gtcataaaat agagtgtctg
       61 ttgatctcat tttcatattc ctttcaggct gaagtgcgtt ttcatcaccc gatgagaaag
      121 aactgaagtg atgtttgcca tagattgcaa gatgccctgt ctcattgtct atcgtggtgc
      181 acctttatac ctgttatacc agatcgaaaa tcacgcaatc catacaacaa aaccagattt
      241 gcaattcgtg tcacaaaata tgtcgatctt tttctaagaa gaagatgcca tgtgaagcca
      301 gacgaacact tgcggtggtc ttcaaaaact aaagatcttc gtttaactat ttgttttata
      361 aataatttat ttagagtcta aacaagggaa gctttgcaag ctaactcagt gagcttggtg
      421 taaatcagtg tttacccgcc atcaggctga gcataattct catcatgaaa tatgtttcct
      481 ggtttgtggc ttgtaactgg tcacttctga agtcgatctg gagaggctta ttgatgttgg
      541 tgttttcagg atgatgtttc acttagtttg tttgccgtat cgcccggcga atggctgtga
      601 ttgaggaagg tttaagtcgt agtgaccaaa gctatattta ccaacgaatg tagatgaaaa
      661 aatcatctcc tgcgttcccc catatctcca ggataaaaag gaatgtaaca atctcatggc
      721 gtaagctgac gaatcagcca ggaataatcg ctagggacct aagaattagc ttgataatag
      781 ccactaagaa attactgtgt tccatgaaat agccattttg tggcaaatgg agttgactaa
      841 taatgtcata tgtgagacgg ctagttgaac gaatattaaa ttttgctgaa ttttttatgt
      901 tgattttact tg
//
LOCUS       DAZFTN010000405          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFTN010000405 DAZFTN010000000
VERSION     DAZFTN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>895
                     /locus_tag="ACHKV2_005574"
     CDS             <1..>895
                     /locus_tag="ACHKV2_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943219.1"
                     /translation="PPEGFESSGSETALGVEVTKAKPVTRTITSANIDRLRVTFGVQS
                     LVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFN
                     IRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNY
                     HIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAA
                     DVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVW
                     NGQTLTFVQDRPSDVVWPYTN"
BASE COUNT          196 a          257 c          276 g          166 t
ORIGIN      
        1 caccgccgga aggttttgag tcatccggct ctgaaaccgc actgggcgtg gaagtgacga
       61 aggcaaagcc ggtgacgcgc accattacgt ccgcgaacat tgaccgcctg cgggttacct
      121 tcggggtgca gtcactggtg cagaccacct cacagggtga ccgtaacccg gcatccgtcc
      181 gcctgctgat tcagctgcag cgtaacggta actgggtgac ggaaaaggat gtcaccatta
      241 acggcaagac cacctcacag ttcctcgctt cggtgattct ggataatctg cctccccgtc
      301 cctttaacat ccggatggtc cgggagacgg cggacagcac cacggaccag ctgcagaaca
      361 gaacgctgtg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
      421 cgattgtggg gctgcaggtg gatgcggagc agtttggcgg tcagcagatg acggtgaact
      481 accatatccg cggtcgcatc atccaggtac cgtcaaacta tgacccggaa aaacgcacgt
      541 acagcggcat ctgggacggc agcctgaaac cggcatacag caacaacccg gcctggtgcc
      601 tgtgggacat gctgactcac ccgcgctacg gcatgggaaa acgcctgggg gccgcggatg
      661 ttgacaagtg ggcgctgtat gccatcgggc agtactgcga ccagacggtc ccggatggtt
      721 tcgggggcac agagccgcgg atgaccttta atgcgtacct gtcacaacag cgtaaggcgt
      781 gggacgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacggccaga
      841 cgctgacgtt cgttcaggac cgcccgtcgg atgtggtgtg gccgtacacc aactg
//
LOCUS       DAZFTN010000406          893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFTN010000406 DAZFTN010000000
VERSION     DAZFTN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>893
                     /locus_tag="ACHKV2_005575"
     CDS             <1..>893
                     /locus_tag="ACHKV2_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6943220.1"
                     /translation="LWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETS
                     QRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQV
                     AVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITD
                     VVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRD
                     ELALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKT
                     RSAGEREEEQAREALLALEAEL"
BASE COUNT          231 a          193 c          301 g          168 t
ORIGIN      
        1 ttgtggaaaa tggccggggt gatcagcgat gaagccggtg gtggtatcag acaggcggca
       61 gaaaatctgg cccgtctggc agaaagcggg aaatataccg ccgggcagct acggatcatg
      121 ggggaaacct ctcagagatg gctgcagacg gtgggggacg atgccgggaa ggtggaaaaa
      181 gcctttgaag ggattgcagc agatccggtg aaggcgctgg cctccctgaa tcagcagtat
      241 aacttcctga gcgtttccca gttacgccat attgatgagc ttgagcgcac gaaaggtaaa
      301 caggttgcgg tgacggaggc gatgtccctg tttgcggatg tcatgaatgc acgtctggag
      361 caacttgata aagcggccac gccggtggaa aaaatctggg acgatgttaa aacctggact
      421 tctgacgcat gggcatggat aggtgatcat acactggggg cactcagtct gatcactgac
      481 gtggtggccg gaaccgttga acaagtgaag ctgctgcttg tgcaggggga tctggcgctg
      541 gctgaattta ttcagtcagc ctgggaaacg acaaagaatg tgcccggcgt tggtgcgttg
      601 tttggtgaac tggcagaaga gaaccgcgta tttattgaga aaacaaaacg cgatgaactg
      661 gcgctgagaa aatccattgc ggaacgggat gcgcgtatac gccaggggga aatggggtac
      721 atcaaccgct cgcgtgcaac aggcgtcagc aaaggtccgg ggcagcagga agccgtcagc
      781 cgtctggctg aagagctgac aggtaaaaag catacatcac cgaaaacgcg ctctgccggg
      841 gagagggaag aggagcaggc aagagaggct ctgcttgccc ttgaagctga gct
//
LOCUS       DAZFTN010000407          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFTN010000407 DAZFTN010000000
VERSION     DAZFTN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..604)
                     /locus_tag="ACHKV2_005576"
     CDS             complement(<1..604)
                     /locus_tag="ACHKV2_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6943221.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EVGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISSSKAEASAANADTSAGDALESAR
                     QAAESAAAAKQSEDASSSSASAAAQKASESSQSAAEAELSR"
     gene            complement(669..>885)
                     /locus_tag="ACHKV2_005577"
     CDS             complement(669..>885)
                     /locus_tag="ACHKV2_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6943222.1"
                     /translation="GDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTESV
                     AIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          172 a          288 c          208 g          217 t
ORIGIN      
        1 ttcttgacaa ttcagcttct gctgcacttt gtgatgactc actggctttt tgagcggccg
       61 cagaagccga ggacgaggac gcatcctctg actgctttgc agcggctgca ctttctgccg
      121 cctgccgggc tgactccaat gcatcccctg ctgaagtgtc agcatttgcc gcgctcgctt
      181 ccgccttact ggatgatatg ccggcattcc tcgctgatgt ctccgcctct ccggcattct
      241 tcttcgcctc ctcagcgtga cgcgccgctt cttccaccat cagttcaaaa cgacgcagtg
      301 cctccggccg gacgtcatcc tccgacatgg caccgagaaa atcattcagc gtccccggtt
      361 gagaatcttc atacacggtg atggtcccgg catgtgacgg cgggaagccc tccaccaaca
      421 gaatgacgct gtactgaccg tactcaacgt ccatgctgta acgaccgact tcatccggat
      481 tttcagaggc caccgtgttc accaccaccg tgctgctggt ccgtctggct ttcagttgaa
      541 tggtgcagtt ctctaccggt tttcctgtgc cgtctttcag tacacctgaa atctttactg
      601 ccatattccc cccacaaaaa agcccgcctg aaccggcggg ctgtcataac actgtgttac
      661 ctggctaatc agaatttata accgacaccc acgatgaaac cgtcagtgcg ccagtcgcca
      721 ctgccggagc cttcataagc aatatcaatg gccacggatt cggtcgggtt aaactgcacg
      781 ccagctcccc acgccagaga cgtgttgctg tggcgaccgt catcacttcc ggtcagcaca
      841 tcgtgcgttt tccccttgtt gtcagttacg cggagataat ccccg
//
LOCUS       DAZFTN010000408          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFTN010000408 DAZFTN010000000
VERSION     DAZFTN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..855
                     /locus_tag="ACHKV2_005578"
     CDS             <1..855
                     /locus_tag="ACHKV2_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943223.1"
                     /translation="NIIKSDFHVGSLKPGSMNGVILEMPPNVCMEPRNSYENKIDEVS
                     SLSESEEHPIDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRAQPQDKI
                     DSYNSALQAFSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKI
                     NITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIFNTPANH
                     ESYEKSLKPVSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPEDAPIENS
                     PVVNSPLV"
BASE COUNT          293 a          132 c          173 g          284 t
ORIGIN      
        1 aatataataa aaagtgattt tcatgttggt tcacttaagc caggtagtat gaatggtgtt
       61 attttagaaa tgccaccaaa tgtctgtatg gaaccacgta attcatatga aaacaaaata
      121 gatgaggttt catctttgtc agagtcagag gaacacccca tagatattca aaaaataaca
      181 gatgcgtttg tgaaggagtt caaggggata ttatttgata aaaatggaag atcttcagag
      241 cttctgttta atttttatga atgttgctat acgtttttac caagagcgca gcctcaagat
      301 aaaatcgata gctataattc agcactgcaa gctttttcca tcttttgttc atctacgttg
      361 acacataata atgtaggctt tgatttcaaa ttatttccag aagtcaagct gtctggagaa
      421 catcttgaaa cggtattcaa atacaaaaat ggcgatgatg tccgggagat agccaaaatt
      481 aacattactc tccaaaaaga agagggtggc ttatataatc tacgtggatt ggattttaag
      541 ggatgcttct tttctggaca gaacttcagt aactatgata ttcaatatgt gaactgggga
      601 atgtcattgt ttgatgttga tactccgtgt atttttaata cgcctgctaa ccatgagagt
      661 tatgaaaaat cattaaaacc tgtaagcgaa aacggtttaa atggagtctt atctgatcgt
      721 aataaaaaaa taaaaatgat cacgggtgtg gcaccattcg atgatatttt atttatggat
      781 gatgactttg atgataactc ccctgaggat gctcccattg agaatagtcc tgttgtgaat
      841 agtcctcttg tataaagttc taaaagcagt tttcaatgat aa
//
LOCUS       DAZFTN010000409          870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFTN010000409 DAZFTN010000000
VERSION     DAZFTN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..641
                     /gene="ipbA"
                     /locus_tag="ACHKV2_005579"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             235..641
                     /gene="ipbA"
                     /locus_tag="ACHKV2_005579"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          268 a          173 c          168 g          261 t
ORIGIN      
        1 aaaaatggct tgtagaaaag cagcacaata aatagttcat atttagtcta ttgcgtagaa
       61 ctactattca aaaaatctta ttaatgagat aatgagatta catcgataac gttctgattg
      121 caggcatact tatctgggtg tgcctctctt ttttacaact gtacacggtg gcgtatgacg
      181 agaaaatatc tcacacagga tgaagtctac aggctgatgg atgcagccca gagcatgtcc
      241 tttcctgaaa gaaatcgctg tctaattatg atggctttca ttcacggttt tagggccagt
      301 gaacttcttg atttacgttt atccgatatt gatgcatcag gaaaacaact taatattcgg
      361 cggattaaaa atggtttctc gacaacacat cctcttcttc ctgatgaata taatttgatc
      421 aagttgtggc ttaagcagag gaagttaatt gaaaacggag ttgaaggaga ctggcttttt
      481 ctctcccgga aacgccgccc tatcagcaga caacattttt ttctatcatt cgtgaggctg
      541 gaagacgtgc aggattagct gtaaaagcac atcctcatat gttacgtcat gcctgtggtt
      601 ttgctctggc tgacaatggt gtcgataccc gactacttca ggattatctg ggtcatcgaa
      661 atatccagca tactgtcaga tacacagcca gtaatgctgc tcgatttaaa ggggtgtgga
      721 aaaaaaagcc tcgttaaaac agtaccttct gattcaaaat gtcagattcc tacgttttcc
      781 tcaattcttc cataaaaaaa cagcccattc aattcaataa aacacataac atgttgatat
      841 ttctcgctaa agtaaaataa ccccatgatt
//
LOCUS       DAZFTN010000410          867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFTN010000410 DAZFTN010000000
VERSION     DAZFTN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>867
                     /locus_tag="ACHKV2_005580"
     CDS             <1..>867
                     /locus_tag="ACHKV2_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6943224.1"
                     /translation="LPDSYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQ
                     DMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEG
                     TFSADAGASQDSARWGVGKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATH
                     AQQPALFTAMLAQFRADLSVFNAQCHGGSAADVPWICGDTTYYWKNTYGTQYNTIYGA
                     YKNRESEGVYFVPFMTDGNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTH
                     FSSWARRSIIPDR"
BASE COUNT          204 a          224 c          258 g          181 t
ORIGIN      
        1 ctgccggatt catacgatgc tccggatccg cgcattaaac agctggcgcg ccgcagtaca
       61 gttacgccgg gtggggctgc ctgcagatat aacgatatta ttccggccga ccactgcctg
      121 catgatgtgc aggatatgag tacgctgaat catccgaagg cagacctgag caaagggcag
      181 tacggctgtg tcggccaggg cttacatatt gccaaaaaac tgcttccgta tatcccgaat
      241 aacgcgggga tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcgcg
      301 gaggggacat tcagtgcgga cgcgggggcc agccaggatt cggcgcgctg gggtgtgggt
      361 aaaccgttat atcaggacct gattgcgcgc actaaagctg cattacagaa gaacccgaaa
      421 aatgtgttgc tggcggtgtg ctggatgcag ggagagtttg acatgagcgc cgccacccac
      481 gcacagcaac ctgcgctgtt tacagccatg ctggcacagt ttcgtgctga cctctccgtg
      541 tttaacgcgc agtgccatgg tggcagtgct gcagatgtgc cgtggatttg tggtgacacg
      601 acgtattact ggaaaaatac ctacggcacc cagtacaaca ccatttacgg ggcgtacaaa
      661 aacagggaga gtgagggcgt ttattttgtg cccttcatga cagacggtaa cggcgtcaat
      721 accgccacta acgcgccggc agaagatccg gatattccgg catcaggata ttacggtgcg
      781 gcatcgagaa cgaatggaaa ccaggtatca tcaaaccgcc cgacacattt cagttcatgg
      841 gcgcgcagga gcattattcc ggatcgt
//
LOCUS       DAZFTN010000411          857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFTN010000411 DAZFTN010000000
VERSION     DAZFTN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..857
                     /locus_tag="ACHKV2_005581"
                     /pseudo
     CDS             40..857
                     /locus_tag="ACHKV2_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          209 a          237 c          283 g          128 t
ORIGIN      
        1 ataatagata tttcttggta aggaaaatat tccccgaaag aggccacatg gggaatactg
       61 ctcccctcac tgatacggga gtaccgttac gacagcgcgg gtaacgtatc aggcgtcacc
      121 agccgggaag attacggacg ggaaacacag cgggagtacc ggcttgaccg gaacggccag
      181 gtcacggcgg tgacagcctc aggcaccggg ctgggctatg gcgaaggcga tgagtcctat
      241 ggctatgaca gctgtggcta cctgaaggcg cagtctgcgg gcgggcaccg gataagtgaa
      301 gagactgacc agtatgccgg aggccaccgg ctgaaacagg ccggaaacac gcagtatgac
      361 tatgacgccg caggccggat ggtcagccgc accagacacc gtgacggcta tcgcccggaa
      421 acagagcggt tccggtggga cagccgggac cagctgaccg ggtattgcag cgcacagggt
      481 gagcagtggg aataccgcca cgacgccagc ggcagacgaa cagagaaacg ctgcgaccgg
      541 aagaaaatcc gcttcacgta cctgtgggac ggcgacagta ttgcggaaat ccgggaatac
      601 cgcgatgatg aactgtacag cgtacggcac ctggtgttta acggctttga gctgataagc
      661 cagcagtgca gccgggtacg gcagccgcac ccgtccgtgg ccccgcagtg ggtgacacga
      721 acgaatcatg cggtgaacga cctgacgggc cgtccgctga tgctctttaa cagtgaaggt
      781 aaaaccgtct ggcggccggg gcagaccagc ctgtgggggc tggcactcag cctgcccgca
      841 gacacagact acccgga
//
LOCUS       DAZFTN010000412          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFTN010000412 DAZFTN010000000
VERSION     DAZFTN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..819
                     /locus_tag="ACHKV2_005582"
     CDS             <1..819
                     /locus_tag="ACHKV2_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO6943225.1"
                     /translation="DVSPALVSKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQ
                     DSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVD
                     GLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQ
                     QALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIRKVIYTTNAIESLNSVIRHA
                     IKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT          214 a          227 c          239 g          172 t
ORIGIN      
        1 gatgtctcac cggcgctggt atcgaaggtc accgatgccg ttatggagca ggtagtcgaa
       61 tggcaaaacc ggccactgga tgcggtctat cccattgttt atcttgactg tattgtcctg
      121 aaggttcggc aggacagtcg cgtaatcaat aaatccgtat tcctcgcact gggtatcaat
      181 atcgaaggcc agaaagagtt actgggtatg tggctggccg aaaacgaggg agcgaagttc
      241 tggctcaatg tgctgactga actaaaaaac cgcggcctga acgatattct catagcctgt
      301 gtcgacgggc tgaaaggctt cccggacgct atcaacacgg tgtatccgga agcccgcatc
      361 cagctgtgca tcgtgcatat ggtgcgcaac agcctgcggt tcgtttcctg gaaggactac
      421 aaagccgtca cccgcgacct gaaagccatc taccaggccc ccacagaaga agcaggccag
      481 caggcgctgg aagcgttcgc cagcgcctgg gacagtcgct acccgcagat aagccggagc
      541 tggcaggcaa actggaccaa cctggcgatg ttcttcgctt acccggcaga tatccgcaaa
      601 gtcatctaca caaccaacgc catcgagtcg ctgaacagcg taatccggca tgccatcaaa
      661 aaacgcaagg tgttcccgac ggatgactca gtgaaaaagg tcgtgtggct ggcaatccag
      721 gcggcttcac agaaatggac gatgccactg cgggactggc gtatggcaat gagccgcttt
      781 attatcgagt tcggtgaccg cctggacggt cacttctgag aaaaggcatt tacacagaat
      841 cgtgtacagg gt
//
LOCUS       DAZFTN010000413          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFTN010000413 DAZFTN010000000
VERSION     DAZFTN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHKV2_005583"
     CDS             complement(<1..143)
                     /locus_tag="ACHKV2_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO6943226.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIAL
                     ERR"
     gene            complement(91..657)
                     /locus_tag="ACHKV2_005584"
     CDS             complement(91..657)
                     /locus_tag="ACHKV2_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6943227.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPESL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          242 a          201 c          135 g          271 t
ORIGIN      
        1 cttcgtcgtt cgagtgctat cttggttcca caaatctcat tacaccaata ttgattttga
       61 tattttggta taaaccattc attgcaacat ttacatttcc ttcgatagat tcgcataagt
      121 gctcctttcg ttgccggaaa aatcaccgta atacttatct cgggcttctt cagcaactag
      181 taccgctaac tccagatcat caaagcatcc gaagtgttta ctcttgccat ggaatcctag
      241 cctaacattc cattttttct gtcgtttgtg ccaagtaacc cctctgcaac ctgatttgct
      301 attctttcgg atccttatat ttcttgaatt ttctattggc aggcattctc ttaaactttc
      361 tggcctattg tcggtcctaa ttccattaac gtggtcaatt tgaccagcag gccaacgatt
      421 atgagttatg taaaaaacta agacgtgagt tttatatcta cgcccatcta tcatgatcat
      481 tgaataaccg ttggaatcaa aagctccagc aacactattt aatgctatcc ttccctgagt
      541 gggaactttc catctaaata ccccggtaga tttatcgaaa cttagtaact caaatatcct
      601 tttaacagtt aaatcttctc ttttacggtt acatcgtctt cgcgctggtt tagccatcgc
      661 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
      721 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      781 aaaagggaag acactacccc cgataattca gaaacaaatc gaaatacatg aactaaagaa
      841 aaaatcaca
//
LOCUS       DAZFTN010000414          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFTN010000414 DAZFTN010000000
VERSION     DAZFTN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>834
                     /locus_tag="ACHKV2_005585"
     CDS             <1..>834
                     /locus_tag="ACHKV2_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6943228.1"
                     /translation="CAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQFLEHYPA
                     AQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPMNVAFDK
                     LAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPE
                     LKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARVHRQH
                     TENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVID
                     N"
BASE COUNT          230 a          205 c          221 g          178 t
ORIGIN      
        1 tttgcgccgc cattccggac gaacaggcaa taaaggaaga aggtgcagtc gctgtagcca
       61 ctgccattga agccggtgat gaacgtcgcg cccgcgcaaa atttcactgg caattcctgg
      121 agcattatcc ggctgctcag gactgcgctt ataaatttct tgtttgcgag gataaacccg
      181 gtataccccg ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg
      241 atgagggggc tgcttccttt gtcccggttg agactgaatc cgatccgatg aacgtcgctt
      301 ttgacaagct ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa
      361 acatcaccgt tgatatggtg attagcgcgc aggaattgtt gcaggaagac atggcaacat
      421 tcgacggaca tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtatccgg
      481 agcttaagct gcatgccatc gggtgggtta agcataaatg taagcctggt gccaaatggc
      541 ccgaaattca ggcagaaatg cgcatctgga aaaaacgtcg cgaaggtgaa cgcaaggaaa
      601 ccggaaaata cacgtctgtt gttgatctcg cccgcgccag agtccaccga cagcacactg
      661 aaaactcagc agaaaaaatc ccccctgtca ctgcagtcat tcgtcgcgaa tataagcaga
      721 catggaaaac actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg
      781 gaaacattga cggcagcatc catcgctggg caaaaaatga agttatcgac aacg
//
LOCUS       DAZFTN010000415          829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFTN010000415 DAZFTN010000000
VERSION     DAZFTN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            187..789
                     /gene="lpfA2"
                     /locus_tag="ACHKV2_005586"
     CDS             187..789
                     /gene="lpfA2"
                     /locus_tag="ACHKV2_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO6943229.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          253 a          160 c          171 g          245 t
ORIGIN      
        1 taacgatata aatttagttt tgcttaacac ctaaattaat ttatctaaat gtataaaata
       61 aaaaccactt gcgccaaagt atattggtag tactatttgg tttctgatca aataccgatc
      121 aggtttatcc aaatttaata aatggataac acacaggaat aatatcaaat taatggagtt
      181 atatttatga aaccaaatat gattgtagga gcattagcgt taacttctgt gtttatggca
      241 ggtcacctac aggcggctga tggaacagtc catttccgtg gtgaaattat tgacagtact
      301 tgcgaagtca ctcctgaaac taaagatcag gtcgttgatt taggcaaagt aaaccgtaca
      361 gcctttagtg gcgtcgatga tgtggctgcc ccgacggctt tttctatcga tctgactcaa
      421 tgcccggaaa cctttaagtc cgccgcaatt cgtttcgatg gtaatgaaga tgctcatggt
      481 aatggcaacc tggcaattgg taccccgctg gataactcta acgatgctgc cgctggtatt
      541 agcccgagtg ataacagtgg ggattatact ggtgcgggtg ccgttagtgc agcgaaaggc
      601 gtagctattc gtttatataa ccgtgcagat aacactcagg tcaagttata tgaaaattct
      661 gcatcaactc cgatttctaa tggtaatgca tccatgaagt tcatggctcg ttatattgct
      721 acggaaacga ctattgaccc tggtacagct aacgccgact cgcagtttac agttgaatat
      781 ataaaataaa tcattcttaa cataatacct taaataaaat ggcgctcgc
//
LOCUS       DAZFTN010000416          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFTN010000416 DAZFTN010000000
VERSION     DAZFTN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..428)
                     /locus_tag="ACHKV2_005587"
     CDS             complement(<1..428)
                     /locus_tag="ACHKV2_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO6943230.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDN"
BASE COUNT          208 a          178 c          205 g          235 t
ORIGIN      
        1 tggttatctg agaaccacga gagagggtag ttgatagata gcgttgattc tggaagctga
       61 agttgttctg ggattttttg actgaaatag cagtcttcca gtttttcgaa cacttcccac
      121 gcccgatcgg tttcgagcat ttttgcgtga cgggctgcgc cgcgttctgt ccagaggatg
      181 agggagcgaa cgttacgggc gattttcaca gaaggtctta aagacatgct gtgcttaaat
      241 tcccgcaact cctcattctc aagtaaaaag tagtgcttac cgataacaaa tcgtccagaa
      301 ttccgattga aattttttcg gatgtaatct ggttctgtgc catataagct ggcaagaagc
      361 tctgtagtca ccacaggtat attactaaat gaaattatgg agagggactc cacggagatg
      421 gttttcatgc tttgcgtcct acttgatatt ttgatttacc ccttttgaga gggtgaccgg
      481 gcgctcaaaa ccgcaagtag tcggcgggca tattcccatt gctggtattg tattagccgc
      541 acgcccggtc ataaaccaag aagactggac acaaaaaaac cgcatgtctg tcgggcgcgg
      601 taaccgctac ttgctaaggt gttttgagca ccatgaggca gaatatatcc ccgttaatgc
      661 agcattgtca aacgatctgt tctttaaaga cttaacgaga agccttttcg ggaatttcga
      721 tctgacagtc ttgaaatatc ctgtagcgtg cggcgcattt cttccgtcag atgcttgaag
      781 gcgttaaatt cggcgacagc ggcgtcaacg ttatagcctt tgctgt
//
LOCUS       DAZFTN010000417          825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFTN010000417 DAZFTN010000000
VERSION     DAZFTN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..268
                     /locus_tag="ACHKV2_005588"
     CDS             <1..268
                     /locus_tag="ACHKV2_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943231.1"
                     /translation="TRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQ
                     SSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            265..>825
                     /locus_tag="ACHKV2_005589"
     CDS             265..>825
                     /locus_tag="ACHKV2_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO6943232.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          203 a          208 c          233 g          181 t
ORIGIN      
        1 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
       61 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
      121 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
      181 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
      241 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
      301 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
      361 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
      421 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
      481 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
      541 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
      601 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
      661 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
      721 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
      781 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcg
//
LOCUS       DAZFTN010000418          815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFTN010000418 DAZFTN010000000
VERSION     DAZFTN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..582
                     /locus_tag="ACHKV2_005590"
     CDS             40..582
                     /locus_tag="ACHKV2_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943233.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            586..>815
                     /locus_tag="ACHKV2_005591"
     CDS             586..>815
                     /locus_tag="ACHKV2_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943234.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAA"
BASE COUNT          197 a          174 c          226 g          218 t
ORIGIN      
        1 ctgcctgcgc ctgtaaactg aaaataagga acaggatata tggcatattt ttatttcaaa
       61 ttagaccgtg tgcagacaaa taaatatttc accaaatatc aacagactgt tttaccgcta
      121 cgcaacagta ttcttcgggc attactgaaa aatacaggcg ctgccggatt gcgcttaaag
      181 ccgtttgcca tggacgtaat cagtgagttt tatttttctg gtgctctgcc tgcgggctgg
      241 cgtaagcgcg atgatgtggc ttttatcggg gacggaccgt gctttattgc cagacctgat
      301 gagtcatgcc ctgaaggacc ggcgattgcc gcaatgattg aagccgctga acgcgagtta
      361 agaaagcgtc ctgatttcct tgtctggctc tgtgaaaagc tgggggtaat gagaatcccc
      421 tccatgttta acatggactc ctggtggacc ccgtcgctct cccgtgatgc cctgtgcgtg
      481 gtgtttaaag taggcgctta tggcagggaa ataaaagggg gtattcctga agaatgccag
      541 gagattaaac attctgaata tgtggcgctg acggaggaat aattcatgat tgatgcaaaa
      601 gtgcttgaag gggttaaaaa ctggctgagt atttacgggc gtctgacctg cggcattctg
      661 gctgaaaaaa tgaatatgcc gccatcctcg atggtttatt ttctgcgtga tgcggtcgat
      721 gccggggtgc tgacggaatg taacggtttt tatgatattc cgcgtccccg cccggtgcag
      781 ccggttcgtc gcaaatgcag ccaggaaggt gcggc
//
LOCUS       DAZFTN010000419          811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFTN010000419 DAZFTN010000000
VERSION     DAZFTN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(134..676)
                     /gene="fimA"
                     /locus_tag="ACHKV2_005592"
     CDS             complement(134..676)
                     /gene="fimA"
                     /locus_tag="ACHKV2_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6943235.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          248 a          168 c          157 g          238 t
ORIGIN      
        1 ttttcattta ataaagcggc atgatacaaa gtgaacatga tttcgattta cattgccacc
       61 ataccgcttc agacaaccgg tacattactt tgttataaga cgtgatatat aattcaaaac
      121 aacgtggttt tgattattcg tatctcataa taaaagtcgc gtcagcattt gcttgccctg
      181 ctgatgcact tgtaccggtg cctttataac gtgcagaaaa atgaagaacg ttggtcccgg
      241 ggatcaagtt ctggttggtt gagaagctat tcccatcagg tttgagaatt gcggatgtat
      301 tatcaagtat ttcaataccg acacccgttg ctgttgttgt attggtactg gatgcaatgg
      361 ccaataaatt atcattgatt gcatcagcct gaccagaaaa tgcaacggca gcattagcgg
      421 caacaacagg atcgcagtcg ttcaactgaa tggtgaatgg aattaatgca gaggtattac
      481 caacagcatt gaaaatatcg gtacgatatt gaccgagtgt gacaacctgg tctgccgagt
      541 cagtattcac tgaacaggca gcattcacca gttcaccttc aaagtgaatt gtaccgccat
      601 ctacaacggc agcataacta cccgtagcgg cgaatagtgc ggcagccagc gcagacgaaa
      661 taaatcttaa tttcatatat attccttcaa tctcatttat cgactccaca tccgtatata
      721 accgattact ttatttaaga cactgatagt agtaaattcc tttttatcct ctaagaatgt
      781 cttaattgaa aatatgcact ctattctaaa a
//
LOCUS       DAZFTN010000420          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFTN010000420 DAZFTN010000000
VERSION     DAZFTN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            217..292
                     /locus_tag="ACHKV2_005593"
     tRNA            217..292
                     /locus_tag="ACHKV2_005593"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:250..252,aa:Ile,seq:cat)
BASE COUNT          232 a          147 c          185 g          246 t
ORIGIN      
        1 gtatcattga atcgatgata actatagatt gtgctaatta ttaacatttg cttgttgact
       61 gcgagtatcg actttgcgga atggaggtgg tgtcgggttt tatgtcgcat gtattgacga
      121 cccaggatgg atgagaacgc ataaaaatct gtgatttaca tcataagatc atcatgttta
      181 ttgcgtgggt tataagtatt gttattgcat acttaaggcc ctttagctca gtggttagag
      241 caggcgactc ataatcgtcc ggtcgctggt tcaagtccag caagggccac caaatcatca
      301 ttcatgtatc aggaagagtt aagggtacaa gggttactcg atggcggtct atatttcacg
      361 attaacgcca tttcaaggaa gaagtgcggt gggtggtttc gaatgagggc tggtgttttg
      421 gtacgtggca cgccactgca atgtatttat ctatcagtac tcattcaaaa gtatttggaa
      481 aaaatgcctg ctatgccaag cgggcaaatt gcttacatgt ggaattaagt aattaacgat
      541 aaagccagcg tattatcatt tattataccc attcatttaa tcaggttaat tactcaaatc
      601 actgaactat tctctgttcg ctcactgatg ataagggagt accacaacca atgtatgtag
      661 aacaatgccg tagagtaagg atgaaatgtc tctgccacgc cttaaacatc tactactgac
      721 tagaacaccc gccgcaggta gatgagtttt gtgagcgtta caggagcgtg gctatgctca
      781 ttaatcaacg ctatttagat ataaatttct
//
LOCUS       DAZFTN010000421          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFTN010000421 DAZFTN010000000
VERSION     DAZFTN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..>809
                     /locus_tag="ACHKV2_005594"
     CDS             54..>809
                     /locus_tag="ACHKV2_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943236.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTI"
BASE COUNT          303 a          160 c          135 g          211 t
ORIGIN      
        1 aattatccaa tttaaaaatc ttagcataaa acagataatg aataaggaat gttatgtcaa
       61 ttgactttac cccaggtatg ataaatacat atcacggcga tatttataac cgcacaacag
      121 ataccgacaa tgttaaaaca ccagacacac cgatgtggcc ttgtgataat cgggaagaac
      181 aacaacccat taattccaca ttctctggag aaggatacaa ccctgaacaa tatgatttag
      241 cgcggcaaca cctcccacaa atttatgcgt gccgcacgaa caccaaatat acagatgcag
      301 actactcaaa agttgtggct caacttgtaa gtcttattac taacattgaa tcgataagtt
      361 cgacaccact tacgcggcag gcacaggaaa tacttaatca aattaataac attagatacg
      421 aaaagaacaa aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg
      481 caatcataac taaaattagc gtagaagagg gaataccagt aagattcagt gtccaaacca
      541 tgttttcaga tactaatttt attgctgaac aacgagctga cttaccgact aatataaaag
      601 atattcaaag cctgtatcaa aaaatgacga agttatatat tgagcacagt gaaaataaaa
      661 acaggatgaa agtctttgcc ggaaccaact ttattgattt caatatgaca ggacagaacc
      721 tgtccgggtt cgttctaacc ttgtcaaggt tttatttcga agatctactt aatataaatt
      781 tcactgacgc aaatttaggt gacactatt
//
LOCUS       DAZFTN010000422          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFTN010000422 DAZFTN010000000
VERSION     DAZFTN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            153..434
                     /locus_tag="ACHKV2_005595"
     CDS             153..434
                     /locus_tag="ACHKV2_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943237.1"
                     /translation="MTEAEILGLIRRVAGISQQADEQATQPDSMTADNYVRVVVEVMR
                     GDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
BASE COUNT          212 a          156 c          203 g          232 t
ORIGIN      
        1 catcatcatc atgtgtgtaa cctgttgttt ttaatgtggt tgatgcaaaa aagatgatga
       61 ttgtggttaa tttttgttcg acatctttta gcgtgacaga ttctttacaa aaaatctgag
      121 cttgttttct tcaccagcgc gatgggggca caatgacaga agccgaaata ctgggattaa
      181 tccgccgcgt cgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagca
      241 tgaccgcaga taattatgtg cgtgtagtgg tggaggtgat gcgcggtgat ggtatccagc
      301 ttaatgatgt ggatatgcgc aatatacgaa ccagagtcct tgagttgctg gcataccgtc
      361 gccgttctca acaacggagg gagagcgcga aaaatactta ccagtggaag acgccggaac
      421 ggctgcggag gtaacttgtt gatattcccg ataacgcaaa attgcgttgg ctggtgggtg
      481 agttgcagat ctgcaactcg atcatgaaat tacggaaact acccgtagtt tgggtagtaa
      541 gagcaacacc cagattttgg ggcttacttg cgatacccaa ataaagggta tcggtggaag
      601 aaatatcgtt tctcatatgt gagttccgag agcggaattc cgcctctgat ttgtcattgt
      661 gatcatgcat agcggcaata attaatattg cttccttttt tgctttttta tcgcttaagc
      721 aggtactaaa tctgggacta tgttgtaggg ggcataactc aatagtacct tataatcatt
      781 tagttacctt ttatttactt ctt
//
LOCUS       DAZFTN010000423          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFTN010000423 DAZFTN010000000
VERSION     DAZFTN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..>793
                     /locus_tag="ACHKV2_005596"
     CDS             103..>793
                     /locus_tag="ACHKV2_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6943238.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQK"
BASE COUNT          263 a          136 c          171 g          223 t
ORIGIN      
        1 ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca catgttaacg
       61 atttaccagt aatgtaaata aattttcgag gagatcattc ctgtgggacg taaatgggcc
      121 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      181 ggtgtagaaa tctatgctgc cgctaaacaa ggtgaacccg atccagaatt aaacacatct
      241 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      301 gcaattgata aagccaaagg cggcggagat gaaacattcg tgcagggccg ttatgaaggc
      361 tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt taaccgtacg
      421 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      481 gtcagctata tgtttgacaa tactggtgtg attgtattta aaggatcaga ccctgaccat
      541 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga agaagaaggt
      601 aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc tctaaaagca
      661 gctggaatca gtgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      721 ctttcaccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      781 gatgtacaaa aag
//
LOCUS       DAZFTN010000424          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFTN010000424 DAZFTN010000000
VERSION     DAZFTN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>788
                     /locus_tag="ACHKV2_005597"
     CDS             <1..>788
                     /locus_tag="ACHKV2_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO6943239.1"
                     /translation="TNSTSFADISIPLLQTICPSITTIIKKTEYYGHQLTNAMTVASV
                     VKPYDFSNLGAINSIDKSVSDVPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHK
                     STNIEFHPQSLLFDRDSKGKCLGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYND
                     NLKLSNRDDFFLRKTQRIITMSNELGNNRLKNAQLEVLELKDPILTEGILYQRRISSL
                     LITTEYHSLALQQISSFWRVTDPNFGHCDFHSLAQALTFIKNIT"
BASE COUNT          285 a          137 c          124 g          242 t
ORIGIN      
        1 aacaaatagt acttcatttg cagatatttc tataccatta ttgcagacca tatgtcccag
       61 cataacaaca ataataaaaa aaacagaata ttatgggcat cagttaacaa atgctatgac
      121 tgttgcttca gtggttaaac catatgactt tagcaatctt ggtgccataa atagtataga
      181 taaatccgtt tctgatgtgc ctgcattgca tacaattgtt gaacaagcaa aatataattt
      241 actttcatgg aatgattttt ataacactca tgcgtcaata tgggatacaa tagcaagaca
      301 gcataaaagt acaaatatcg aatttcaccc acaaagtctt ctttttgata gagattcgaa
      361 aggaaagtgc cttggtttat ctcttctgta tcttgataca ggggggtacg gaggcggata
      421 tcaaaaactg aggcacaata ttgacactgc cagcacatta tatcaaacaa aatacaatga
      481 caatctaaaa ttatctaacc gtgatgattt ctttttaaga aaaacacagc gtatcattac
      541 aatgtccaat gagttaggta ataacaggtt aaaaaatgca cagttagagg ttctggaatt
      601 gaaagaccct atactaactg aaggcatatt ataccagcgt cgtatatcat ctcttttaat
      661 aacaacagaa tatcattcac ttgcattaca acaaatatct tctttttgga gagtaactga
      721 tcctaatttt ggtcattgtg actttcattc cttagcacag gctttaacgt ttataaaaaa
      781 tataacga
//
LOCUS       DAZFTN010000425          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFTN010000425 DAZFTN010000000
VERSION     DAZFTN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>788
                     /locus_tag="ACHKV2_005598"
     CDS             <1..>788
                     /locus_tag="ACHKV2_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943240.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPE
                     KEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFM
                     LRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRI
                     AAPAAPSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTG
                     LYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASG"
BASE COUNT          159 a          219 c          256 g          154 t
ORIGIN      
        1 gatggcgtgg agacatacgg tgtgtggggg ctctccctgc cgtcactgcg ccgtcgcctg
       61 ttccgctgtg tctccgtccg ggaaaacacg gacggcacct ttgccatcac ggcggtgcag
      121 cacgtaccgg aaaaagaagc catcgtggat aacggtgccc gctttgagcc gcagtcaggc
      181 accctgaaca gcgtcatccc tccggcagtg cagcacctga cggtggaggt gagcgcagct
      241 gacggtcagt atctggcgca ggcgaaatgg gacacgccga aggtggtgaa gggcgtgagc
      301 tttatgcttc gcctgaccgt ggccgcggat gacggcagtg agcggctggt cagcacggcc
      361 cggacgacgg aaaccacata ccgcttcacg caactggcgc cggggaacta caggctgaca
      421 gtccgggcgg taaatgcgtg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt
      481 gccgcaccgg cagcgccgtc acagattgag ctgacaccgg gctattttca gataacagcg
      541 gtcccgcgtc ttgcggtgta tgacccgacg gtacagtttg agttctggtt ttcggaaacg
      601 cggattaccg atatcaggca ggttgaaacc acagcccgct accttggcac ggggctgtac
      661 tggatagccg ccagtatcaa tatcaaaccg ggccatgatt attactttta tatccgcagt
      721 gtgaacaccg ttggcaaatc ggcatttgtg gaggctgttg gccagccgag tgatgatgca
      781 tccggcta
//
LOCUS       DAZFTN010000426          787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFTN010000426 DAZFTN010000000
VERSION     DAZFTN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>787
                     /locus_tag="ACHKV2_005599"
     CDS             <1..>787
                     /locus_tag="ACHKV2_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6943241.1"
                     /translation="VAVATAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKP
                     GIPRPALDSWDAEYMQENRWDEASASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDT
                     CENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKP
                     GAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAI
                     HREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDN"
BASE COUNT          219 a          193 c          202 g          173 t
ORIGIN      
        1 gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac
       61 tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt tcttgtttgc
      121 gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
      181 gaaaaccgct gggatgaggc gtctgcttcc tttgtcccgg tcgagactga atccgatccg
      241 atgaacgtca cttttgacaa cctggcccct gaagtacaga acgctgtcat ggttaagttc
      301 gacacatgtg aaaacatcac tgttgatatg gtgattagcg cacaggaatt gttgcaggaa
      361 gacatggcaa cattcgacgg ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac
      421 gctatgtatc cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct
      481 ggtgccaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
      541 gaacgcaagg aagccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat
      601 caacagcaca ctgaaaattc aacaggaaaa atcaacccgg tcattgctgc cattcatcgc
      661 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
      721 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc
      781 gacaacg
//
LOCUS       DAZFTN010000427          777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFTN010000427 DAZFTN010000000
VERSION     DAZFTN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..86)
                     /locus_tag="ACHKV2_005600"
     CDS             complement(<1..86)
                     /locus_tag="ACHKV2_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6943242.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQS"
     gene            complement(154..>777)
                     /locus_tag="ACHKV2_005601"
     CDS             complement(154..>777)
                     /locus_tag="ACHKV2_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943243.1"
                     /translation="MNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVI
                     DMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          186 a          210 c          183 g          198 t
ORIGIN      
        1 gactgatgtt ctgctgcact cgctggcgta gcagcggcga cctgccagac tactgcggac
       61 aaaataacag cacataattt acgcataatt acctctcgct tttctgcaat aaaaaaggcg
      121 ccatttctgg cgcccgtatc tgggttataa aattcagcta atcgtgatgc ctgcagtggc
      181 tttcttcatc accacaacca gcaaatcgct gatacttgct gtgggatacc agttatttac
      241 cagccatgct gacaccgaaa actccagtgt catgtgaccg tgaccggcag gcatatcaat
      301 aacaccactg taaatcagcg tattatccag cgcggtacgg ttataaattt cagcaccgtt
      361 tttccgcact atcagacggc atgaggagta aatatcagta tgctctttct catgtttagc
      421 gccgctgaat gccaccgccg gaataacaat ctgccggtca aacggctgat cgtcataaac
      481 cctgacggta atggttcctg atggccaccg ctccggtgca cgggagtccc gggggaaagc
      541 tttgcccact gttttaacga gatcgccttc aatctggttc gcggacaatt ttcccagaac
      601 ccgacagttc tcgttaatcg tgacgttgtt gagcgtcccg gagttcgcat tcacgttacc
      661 gctgatatcg gcatttttcg ccgtcagccg cccgtccggt gtcagggaaa atgccggagg
      721 attaccgccg ctggtaatgg tgggagccgt cagatatttc aggaacactt cattcat
//
LOCUS       DAZFTN010000428          770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFTN010000428 DAZFTN010000000
VERSION     DAZFTN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(371..>770)
                     /locus_tag="ACHKV2_005602"
     CDS             complement(371..>770)
                     /locus_tag="ACHKV2_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO6943244.1"
                     /translation="WNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFC
                     KYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFT
                     CTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          284 a          174 c           97 g          215 t
ORIGIN      
        1 gcaaaacaaa atcaattatc acattcaaaa aaatactgac aacaaagata ctcttcagac
       61 aaagttgtca tatgtaaatt ttcccttaaa acgttgctca agaaaaatat aaaaatcata
      121 tgccctaatc aacatcctca agcactacga tatatgcagt atcctattca tatattacca
      181 ccatatcaga caaatcgaat cacaacgagt tctatcgtac tctttcatct tcgacactgt
      241 aaattttaag aacattcggc tcatgccact aaaccaagac ataataacaa cggagcaata
      301 aatgttccca ctgaacatgt gcaaacctaa aaagacagtg ttacaataat attcatccat
      361 ggtctctaaa tcaacgccac gcaacaggat aatacaatga aaatatttca ggaaaagatt
      421 cttttgcatg ctccgttagc atatcagtca gcacagcggt acaagtaaaa acattatttc
      481 ttccttgtag cctgtctaaa atatcattgt aataataaac tgagccaaat acctgaggat
      541 ctgttttata agcttgttca atcaatccat ttgcatatct ccgtagaatc tcaggagaat
      601 catactctgt accaaacata tcactcgatg agtatttaca gaaaaccccc cctaaacaaa
      661 ataatatttc agcctgtttt cttagtggca tatctttcaa tacatggctc cccataatca
      721 tatctacatg ctgagcgttc agttcccaac catcaagaaa gggattccag
//
LOCUS       DAZFTN010000429          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFTN010000429 DAZFTN010000000
VERSION     DAZFTN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..411
                     /locus_tag="ACHKV2_005603"
     CDS             <1..411
                     /locus_tag="ACHKV2_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943245.1"
                     /translation="AIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVRLTATVR
                     HWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILAPRDGFI
                     VPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            401..>756
                     /locus_tag="ACHKV2_005604"
     CDS             401..>756
                     /locus_tag="ACHKV2_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6943246.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGR"
BASE COUNT          157 a          204 c          244 g          151 t
ORIGIN      
        1 gccatccacc gttacagacc gaaaattgtg ctggcggatc atgctgttga tgcagacggt
       61 gcacgccaga gggctgtccg ggaaatgcgc cgggcggttg cccgctctgt gcgcctgaca
      121 gccaccgtgc ggcactggtt tcgggagaac ggccagttgt gggatatcaa cctgctgacg
      181 gctgtcacgg ctccccgcac cggagtggaa gagcgtgatc ttcttgtctg tcaggtggag
      241 ttttcgctgg atgcaaatca cggcgaaacc acccgtctga ttctggcacc ccgtgacggc
      301 tttattgttc cggcagagcc gggaaacagc ggaagcggaa atgcgggtga cgtggacgcc
      361 ttcgtgcggg cacagatgaa aaaacagggg attaaattca atgatgaatg acgaagtcat
      421 cagccgcctt ctggcccccg tgatgcgggg tgttcgtctg ctgttcgggc gtggtgtact
      481 gaccggcaca acggacacgc tgaaaatcca gaatgtgcaa atcaccggta tggatggtga
      541 aacctttgat gacgttgaac gcccccagca gtacgggcaa atcagcgtcc ccctgcctgg
      601 tgcggaagtt tttctggcct gtgctggcgg acaacgggat caggccgtgg tgcttgtggt
      661 ggaagaccgc cgcagtcgcc cgaccggact taccgccgga gataccggcg tgtatcacca
      721 tgaggggcac cgtatacgtc tgacaaagaa cggccg
//
LOCUS       DAZFTN010000430          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFTN010000430 DAZFTN010000000
VERSION     DAZFTN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHKV2_005605"
     CDS             complement(<1..293)
                     /locus_tag="ACHKV2_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6943247.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQR"
     gene            355..750
                     /locus_tag="ACHKV2_005606"
                     /pseudo
     CDS             355..750
                     /locus_tag="ACHKV2_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          201 a          190 c          176 g          183 t
ORIGIN      
        1 ttacgctgaa cttcacgtga gatcgtcgaa ggactgcgat tcagcgcagt agctatcgca
       61 cgaatgctca ttttggctga caaaccagct cgtatctcct cgcgctcaga cagtgtcagg
      121 tgagctacag cccgcttacg ctcatggggt tttatgccgc cagtatccct taacatagtg
      181 aagatcgttc cgggttttga acccaggata ttcgctattt cactgaagcc tgttccgttc
      241 ttccatagtt caaaaacaga ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa
      301 acctccgcaa ccccatgttt tcacataact gttgcgttga ccaattgaat ctacaacagc
      361 gtattttgca caggagtcgc tgaaaaatac gcgttaatcg aacaatggcg acaacaattt
      421 cccattgaag cgatgtgtca ggtatttggt gtatccagga gcggttatta caactgggta
      481 cagcatgaac cctcagacag aaaacaaagt gatgagcggc taaaactgga gattaaggtg
      541 gcacatatcc gcactcgcga aacatatgga acccggcggc tccagacgga gctggcagag
      601 aatggcatca tcgttggtcg tgaccgactg gcacgtcttc gtaaggagct aaggctacgc
      661 tgtaagcaga aacgcaagtt cagggcgact acgaactcga accacaatct gccagttgcg
      721 ccaaatctgc tgaaccagac gttcgctcct
//
LOCUS       DAZFTN010000431          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFTN010000431 DAZFTN010000000
VERSION     DAZFTN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>745
                     /locus_tag="ACHKV2_005607"
     CDS             <1..>745
                     /locus_tag="ACHKV2_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO6943248.1"
                     /translation="NACLKLYNLAHTATKHPLKSHDSDNLLFKKLFSPSKLMAIIGED
                     IPLISEKQSLSKVLLNDKNNELSDGTNFWDKNRQLTTDEIACYLKKIAANAKNTQVNY
                     PTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIVNEKNNT
                     QQIKCIIFNSLRALGHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPEIIFL
                     HADLQQYLSQSCGAFVCMAAQEVIEQMESN"
BASE COUNT          280 a          157 c          115 g          193 t
ORIGIN      
        1 aaatgcctgt ttaaaattat ataatttggc acataccgca accaaacacc ccctcaaatc
       61 acatgattca gataatctcc tgttcaaaaa actattctcc ccttcgaaat taatggcaat
      121 tatcggtgag gacattcctc tcatatcaga aaaacagtca ctttcaaagg tacttttaaa
      181 tgataagaat aatgaactga gcgatgggac aaacttctgg gataaaaatc gtcaattaac
      241 cacagatgaa atagcttgct atcttaaaaa gatcgccgcc aatgcaaaaa atactcaagt
      301 caattatcct actgatttct acctccccaa ttccaacagc acttacctgg aagtcgctct
      361 caatgataat attaagagcg atccatcatg gccgaaagaa gtccagttat tccccataaa
      421 tactggcgga cactggatat tagtttcgct acagaaaata gttaatgaaa aaaacaacac
      481 acaacaaata aaatgcatca tattcaattc attacgtgca ctaggccatg aaaaagaaaa
      541 ttcacttaag cgtatcatta acagtttcaa tagtttcaat tgtgacccca cgagagaaac
      601 gccgaataat aagaatataa cagatcattt aactgaacca gagataatat ttttacatgc
      661 cgatcttcag caatacttaa gccaaagttg cggtgcattt gtgtgcatgg cagcccagga
      721 agtgattgaa caaatggaaa gcaat
//
LOCUS       DAZFTN010000432          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFTN010000432 DAZFTN010000000
VERSION     DAZFTN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..463)
                     /locus_tag="ACHKV2_005608"
     CDS             complement(<1..463)
                     /locus_tag="ACHKV2_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO6943249.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGE"
     gene            complement(496..573)
                     /locus_tag="ACHKV2_005609"
                     /pseudo
     CDS             complement(496..573)
                     /locus_tag="ACHKV2_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(575..>739)
                     /locus_tag="ACHKV2_005610"
     CDS             complement(575..>739)
                     /locus_tag="ACHKV2_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6943250.1"
                     /translation="DILESDENGIIPEQVRVITQVVILDADKKQIQCVVRPLQILRAD
                     GTWENIGGMK"
BASE COUNT          208 a          219 c          117 g          195 t
ORIGIN      
        1 cttcaccagt caaacgagaa aatgcagcgg catcaaataa agtacataca tctgaaccat
       61 ctgaattctt cacaaaaaca cctttttcag gctgctccag tgtaattgga cattgaagcg
      121 catcaccagg acattgaagt ttttcaatat cgacagtaaa ggcacactcc gatatttttt
      181 cctgcaaaga gtttcgccct cccattccag ggagcgcact taatatcctg tttctgagca
      241 cattaactcc atcattaaaa gcaatattca ataattctgc ataaacccgt ccctgccctt
      301 cactttctgc taaaaaacgg cctgtatctg gcatataaga aactctaacc ctctcccctc
      361 ccaggagaaa tccaccaatc tgcccctcca ttacctgctg acgcagctcc atcatctcat
      421 tcacgaatga agccgatgac acatatgata ttaatccagg cattaccatg ctccttcata
      481 cggataaata ttccctcaag tcttcattaa caaacatcca ccggacatga caacaaaaac
      541 cggagccgga ctccggtttt gtgaagctgt cgggttactt catcccgcca atattttccc
      601 acgtcccgtc agcacgcagg atttgcagcg gtcttaccac acactgtatc tgctttttat
      661 ccgcatccag tatcaccacc tgcgtgatta ccctgacctg ctccgggata atgccattct
      721 catctgactc caggatgtc
//
LOCUS       DAZFTN010000433          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFTN010000433 DAZFTN010000000
VERSION     DAZFTN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            137..706
                     /locus_tag="ACHKV2_005611"
     CDS             137..706
                     /locus_tag="ACHKV2_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO6943251.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          213 a          154 c          190 g          182 t
ORIGIN      
        1 tttctgcgca tctcacgcgc atctcacaac acagaacctt tcaggatgac ccttgaggat
       61 accggtttgg ctgtcggtgc ctttctgtgg gctggattcc tgtgagacaa ggttcatcac
      121 taaaaggaaa taaccgatga atatgatggc cgtgccgttt cacggcaact ctctttatgt
      181 agttaaccat aatggcgaac catacgttcc catgaaacct gtcgttgcgg ggatggggct
      241 ggcctggcaa tcacagttgg ctaagttaag acagcgtttt gcgtcaacta taacggaaat
      301 cgttatggtt gctgaggatg ggaaacaacg caatatggtg tccatgccac ttcgaaaact
      361 tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa cccgaaatcc gcgataaggt
      421 catccggtat caggaagagt gcgacgatgt tctttacgag tactggacga agggttttgt
      481 cgttaatccc cgtaaaatga gcgtgatgga agaactcaac caggcttgtg ctgacatgaa
      541 acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga aacaggttaa
      601 agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa gcgaatatgc tgattgattt
      661 tgtcctggct gatacaggca aagggaaaat aacaaaggcg gattgatggg gtggctaatg
      721 atatcagata aactcataa
//
LOCUS       DAZFTN010000434          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFTN010000434 DAZFTN010000000
VERSION     DAZFTN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..411)
                     /gene="yhaB"
                     /locus_tag="ACHKV2_005612"
     CDS             complement(<1..411)
                     /gene="yhaB"
                     /locus_tag="ACHKV2_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO6943252.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKY"
     gene            complement(667..>734)
                     /locus_tag="ACHKV2_005613"
     CDS             complement(667..>734)
                     /locus_tag="ACHKV2_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO6943253.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          217 a          143 c          136 g          238 t
ORIGIN      
        1 atacttagaa atcgtctcct taatacattc cagctctttt ttattgacgg ctgagcaagt
       61 acatttttcg ccaaaaggtg caaaagtgcc tacatctaat gtttgtatga tataatcgtc
      121 atgttctcct gtagaaatat ctatgatgaa tggactcata ttttccggtt ggattcggat
      181 catgacagaa ggaacatcgc cttgatagaa attcaatgca gtaattgcag ataatactct
      241 ccgcagttca ttacttatac tgtaaatttt attaacgtca ttggtggaga caatttctgc
      301 aaactttctt tttaccgtcc aataatcaat atttctattg tgattgttaa ccactgcgtg
      361 cattggtggg attgaagggt gaaaaatatg tgcaattggg aagcctttca ttgatgcctg
      421 ccttgtcatc atgacaaaca ataatagatg cttattttcc tcgcagggtt ctttcgcact
      481 atcaaaaaac gctcatttac ccggcgttac agtaaacgaa gcaacattct gtggcctacg
      541 ccgatcaaaa gctctgtgaa aatttccctt gttgtctttg tctgacctta tcgcggcgat
      601 cagcaattat tcgttttgtg atcctcaaca caaaatgcta ttcgtcgcct atgaaggaag
      661 aagaatttat ggaaaacgat tgcattcaca aaatgacgtc ctgtcagaag agaaaaagtt
      721 gacgttaatc aatt
//
LOCUS       DAZFTN010000435          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFTN010000435 DAZFTN010000000
VERSION     DAZFTN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..576)
                     /locus_tag="ACHKV2_005614"
     CDS             complement(34..576)
                     /locus_tag="ACHKV2_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6943254.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT          247 a          141 c          116 g          229 t
ORIGIN      
        1 ttactgaaaa aacaagatga ttattttaaa tatttaatcc tgggcaacac gcataaccaa
       61 tgaagagtta actgtaccag atgcaacacc actgtcagtc aacttcagat attttgcctt
      121 aaacggtatt gcatattgtc catcagcagt tttataactt tcaatattaa atggtgcgga
      181 aatatccagt acgtcactgc tatctcccaa atagagatag tatccaactg tgtcattact
      241 actggatatt gccttatcat taccaagaat tttctcgttg gtgatcaacg aaagttttac
      301 gccatcaata gtcttcggac aagtaatttt cagcatactg ctggttacac caaccgcgcc
      361 atcataagtt gaatatttcg ctttaccatc aaatacagtg gtgggtacag tcggcatatt
      421 aatactgact gaatcaccgc cagcgacaaa tgtacatggt gattctgcaa tattaccttt
      481 aatggttaaa gttccggtat ctacttcagc agcgaaaata ccagcactca taaatacaga
      541 agataagaca gcgacagcaa taattgaatg tttcatgatg cattccttaa taagtttatt
      601 ttgttttgtt aagagtctgt atctcttgga aacaaatttc tcacatcaaa aaggttgccg
      661 caatgtaaac atattgtttt gttgatttaa aacaaattaa ctaatgcaat ataaatacaa
      721 caaataattc gct
//
LOCUS       DAZFTN010000436          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFTN010000436 DAZFTN010000000
VERSION     DAZFTN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..202
                     /locus_tag="ACHKV2_005615"
     CDS             2..202
                     /locus_tag="ACHKV2_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6943255.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            156..>732
                     /locus_tag="ACHKV2_005616"
     CDS             156..>732
                     /locus_tag="ACHKV2_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO6943256.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHL"
BASE COUNT          205 a          171 c          182 g          174 t
ORIGIN      
        1 catgtatcga aacatacggt gccgacactg caataagcta ctggcacggg ccagtttcag
       61 ttatctggaa gtaaaatgtc cgcgctgtaa aaccctcaat caaatcacat ctccgagcgc
      121 cacagagcac cccacataca caaggaaatc ctgtcgtggg gaacagaaag caagtcacat
      181 cccgaatcat cagcacgcct gaacttattc gctataacga caatatcgtt ggttatggct
      241 cccgtgagtt gcgggttgag acaataagtt gctggctggc cagactggtc atcgtcaata
      301 aacattacag ccaccgtttc gtaaataact cctaccttca tctgggcata ttttcagaac
      361 gggaacttgt tggcgtaatg caatggggtt acgcccttaa cccaaacagt ggtgcacgcg
      421 tcgtaacggg tacgcagaac cgagaatata tggagcttaa ccggctatgg ctgcatgact
      481 gtatgccgag aaactccgaa tcaagggcaa tcagttacgc gctcaagcta atcaggcagc
      541 tttatccgca ggtacagtgg gttcagtcgt ttgcagatga gcgttgtggc tgtcttggtg
      601 tcgtgtatca ggcaagcaat tttgattatg taggcagcca tgaatcaata ttctatgaac
      661 ttgatggcga gtggtatcac gagatttgca ggaatgccat taaacgaggc ggacaacgag
      721 gtgaacatct ga
//
LOCUS       DAZFTN010000437          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFTN010000437 DAZFTN010000000
VERSION     DAZFTN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..324
                     /locus_tag="ACHKV2_005617"
                     /pseudo
     CDS             37..324
                     /locus_tag="ACHKV2_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          266 a          148 c          109 g          208 t
ORIGIN      
        1 caagagtctc tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac
       61 aatcatccca ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacacc
      121 aaaactacta caaaacttga atatttaaac ttaagcaata acaatttatt gccaaccaat
      181 gacattgatc aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac
      241 aatgattcac ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact
      301 aatgaagtga ccattgattt atcttataat ctggcaatca caaatatcga taccagtgat
      361 gaacatcttg tagaagtaag cgataattca gaaggtaatt atataaaaga caatgactca
      421 atgtctattc gttatagatc aaaatattat tccagagatt ccgccttaat agaagaagaa
      481 acaatatttt ctgacgcaga actaaaagct attctgccta tgcgtcgcat gtacggggtt
      541 agtgactata agtcaaattc ctcttctcta tcctcacact cgggactcaa ggacccaacg
      601 ggcacacccg tctgttatta tattcataat gagagtaaac cttccttagg ttatggtcca
      661 acatccaata attggttaag ccaatccttt acaacagagt tataaaacac atcctggttt
      721 atcaagggtg a
//
LOCUS       DAZFTN010000438          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFTN010000438 DAZFTN010000000
VERSION     DAZFTN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>731
                     /gene="rfaL"
                     /locus_tag="ACHKV2_005618"
     CDS             <1..>731
                     /gene="rfaL"
                     /locus_tag="ACHKV2_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO6943257.1"
                     /translation="PIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIM
                     PFADYRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLS
                     VLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANG
                     TQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTG
                     LLGLVSLMMLYCAILKECIKNGVKN"
BASE COUNT          217 a          119 c          146 g          249 t
ORIGIN      
        1 ccgatcatat tgagagacga gaaaagagaa gatgtcgaaa aaatcgtttt cttctcattt
       61 attagtgcgt tgggcttacg ctgtttttct gaattgatta cctattataa ggactatcaa
      121 caagggataa tgccattcgc agattataga caccgtagca tttctgactc gatggtcttt
      181 ttattccctg cattgttaaa tctctggctt atcaaatcag caaaataccg catttctttt
      241 gtggttctaa gcgttatttt tatttttctg atattaggaa ctttatccag aggggcctgg
      301 ctttccgtgt tagtcattgg attaatatgg attctgatgt ttaaacaatg gaagttacta
      361 ttagtaggag taatggttgc catcattgca ttgtcggtta ttttcacaca taaggagatg
      421 accgcaaagc taacgtataa acttcaacaa actaatagtt cttatcgcta tgcaaatggt
      481 actcaaggca gcgcactcga tctaatatta gaaaatcctg ttattggtta tggttacggt
      541 aacgttgcat ataaagatgt ctataataaa cgtgtcattg attatccaga atggaccttt
      601 agacaatcaa tagggccaca taattttgcg ctattcatct ggtttggcac tggtttatta
      661 gggctggtaa gtcttatgat gctatactgt gcaatattga aagagtgtat aaaaaatggc
      721 gtcaagaata a
//
LOCUS       DAZFTN010000439          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFTN010000439 DAZFTN010000000
VERSION     DAZFTN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..208
                     /locus_tag="ACHKV2_005619"
                     /pseudo
     CDS             1..208
                     /locus_tag="ACHKV2_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            259..561
                     /gene="bamE"
                     /locus_tag="ACHKV2_005620"
     CDS             259..561
                     /gene="bamE"
                     /locus_tag="ACHKV2_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6943258.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
     gene            complement(637..>731)
                     /locus_tag="ACHKV2_005621"
     CDS             complement(637..>731)
                     /locus_tag="ACHKV2_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943259.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
BASE COUNT          233 a          163 c          149 g          186 t
ORIGIN      
        1 tgaagcgcta caccacctga atatacagca tctgcgacgg tcgcatagcc ttcgccatgg
       61 caggcgtcat acccgcaaag gcgaaagagg tacgattaac atagtgaacg gaacaccaat
      121 tcacgaacgt tcccgaaata tcgataacag acgctctcta gggcattggg agggcgattt
      181 agtctcaggt acaaaaaact ctcatataga ttcgtttttc tgaccttggc aggttgttct
      241 tcagtaggca accagtcatt gaaaaatgag acgcaggaaa gtgtgaaaac caaaattgtt
      301 aaaggcaaaa ctacaaaaca ggacgtgtta gcatcgttcg gtgaacctga cagccgttct
      361 ttgatcgatg gtgaagaaca atggtcatac actatgtata acagccagtc caaagcaacc
      421 tctttcatcc ccgttgtggg actgcttgca ggtggcgcag actcacaaac taaatctctg
      481 acagtttctt tcaaaggcga aaaagtcagc acatacatct ttaatgctgg aacaagcaac
      541 gtgaagactg gcatttttta gataaaagac aaaaccagca tcaagggaaa atttagcccg
      601 cagttgatac ctacgggctt tttacagaaa cataaatcac tttttaaaga aagtgattat
      661 ctttttcagc ctcccccaaa ctttactgtt catttctaca gtctcagtat tttcctttaa
      721 agaggatctt a
//
LOCUS       DAZFTN010000440          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFTN010000440 DAZFTN010000000
VERSION     DAZFTN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..100
                     /locus_tag="ACHKV2_005622"
     CDS             <1..100
                     /locus_tag="ACHKV2_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6943260.1"
                     /translation="CVKPPAPPAWIMQPAPDWQTPLNGIISSSENG"
     gene            61..162
                     /locus_tag="ACHKV2_005623"
                     /pseudo
     CDS             61..162
                     /locus_tag="ACHKV2_005623"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            complement(525..>722)
                     /locus_tag="ACHKV2_005624"
     CDS             complement(525..>722)
                     /locus_tag="ACHKV2_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6943261.1"
                     /translation="TTRNIRFPNQMIEQINIALDQKGSENFSAWVIESCRRELAADIK
                     YARQLTIKKNDTQYALRWLFI"
BASE COUNT          190 a          157 c          179 g          196 t
ORIGIN      
        1 gtgcgtgaag ccaccggcac ctccggcgtg gataatgcaa ccggcccccg actggcagac
       61 accgctgaac gggattattt catcctcaga gaacggttga tgacaatgca gaagcagctg
      121 gaaggggcac aggactatat ccgcactcag tgcctgaact aagttttgct gatgcgccgt
      181 atcgtcgccg tattcctgca ttaacagaga ccgcagcccg acagggagac tcctctgcga
      241 gagtgtgcgg ggataatcaa aaacgataca caccggggtt taccgcgtaa acggagcgcg
      301 gcgttctccc ctcatggtcg cccgtccggt gcgatggtgg aagaaactgg aatctgttca
      361 ataaaaaaac tgccgtgttg gagtcacagc agtaatgtac tgattgggta gaagattatt
      421 attgttatgc tttattttta ttctatatgg ctgattattt caattcggaa ttaatacagc
      481 taatgtctgt gagtttttat aaattcagca atataaagaa atagttatat gaacagccat
      541 cgcagagcat actgtgtatc attctttttt atagtcaact gacgggcata ttttatgtct
      601 gctgccagct cccggcggca agattcaatg acccacgcag aaaaattttc tgaacctttc
      661 tggtcaagag cgatgttaat ttgttcaatc atctggtttg gaaatcggat gttgcgggtt
      721 gt
//
LOCUS       DAZFTN010000441          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFTN010000441 DAZFTN010000000
VERSION     DAZFTN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(30..680)
                     /locus_tag="ACHKV2_005625"
     CDS             complement(30..680)
                     /locus_tag="ACHKV2_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6943262.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          201 a          138 c          142 g          235 t
ORIGIN      
        1 aatatgcttc ttatattgtg cttttatttt taaatactgt tttgttgaag tgggtatatg
       61 ggtaatgcgt aaaaacattg ttttgttata ttaaaatgac acttgtcttt gcttataatc
      121 atttctggag caaaggggtc acggctgagg gggtggagag cgttacgcag gataagttca
      181 gtcaatgctt cctgatcata taatgcacat atttctgaat ttcgagcatt tctgacaaaa
      241 acacctgtct caggaatatg tagcgttatc ggacaattaa gatgttcctc agtacaggaa
      301 aagtcttgta aactaacggg aaatgcattc gaatttattc gatctagtag tgtttttatg
      361 acgctctcct gcacaagcgg ggcagcttgc ctctgctcca gatataagct gtatgcctgc
      421 agaaaagtgt tacctccgtt aagttgtctt gtcaggcggt ctgcgagatg atgagcctct
      481 cttgccccaa gatggtccca aagtccacca agaataggct caacactaaa accatccata
      541 aactggatat aatgaagcct catattacgg cctcctatag taatagtgtc attctggttg
      601 cttcttgcta tatgtcgtaa agcctccagc tcattttccg gcaatacact tccattagaa
      661 aaatttaata tgactggcat attattatcc tttttaatcc atattgtgaa ctatat
//
LOCUS       DAZFTN010000442          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFTN010000442 DAZFTN010000000
VERSION     DAZFTN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..>706
                     /locus_tag="ACHKV2_005626"
     CDS             29..>706
                     /locus_tag="ACHKV2_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFO6943263.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPEN"
BASE COUNT          278 a          129 c          130 g          169 t
ORIGIN      
        1 aaataatttt ttaagcggaa atgtaattat gagactgcca gagaaagttc tttttcctcc
       61 tgtcactagt ggcctgtcag ggcaggaaaa acaaaaaaaa ccgaagagca ttaccggatt
      121 tcaggaaaat tatcaacgca atatcaggcc aatcaaaaca gcatcagaag cccgactacg
      181 cttctttgat aaaatggttt cgaaagaaaa ctctctggaa gatgttgttt ctttaggtga
      241 aatgattcag aaggaaattt atgggcatga acaaagaaca ttttcaccag ttcatcatac
      301 aggtaactgg aaatcatcat tgttacacaa cgcgctcctt ggtctggcaa atgtttataa
      361 tggcttacgg gaaacagaat accctaacac tttcaacaga gatggtataa aaagtactaa
      421 ctcttttaga gataacttat tgacaaaaac aagaactccc agagataatt ttgaggaagg
      481 aataaaacat cctgaacatg caacaatacc atatgacaac gacaatgaaa gtaataaatt
      541 gctaaaagca ggaaagatag ctggtaacaa taacgagctg ttgatggaaa taaaaaagga
      601 atcccaaagc gaccatcaaa tccccctgtc agataagttc ctgaaaagga aaaaacgatc
      661 tcctgtagct gaagataaag ttcaaaactc gttaacacca gaaaat
//
LOCUS       DAZFTN010000443          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFTN010000443 DAZFTN010000000
VERSION     DAZFTN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            255..470
                     /locus_tag="ACHKV2_005627"
     CDS             255..470
                     /locus_tag="ACHKV2_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6943264.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            475..>702
                     /locus_tag="ACHKV2_005628"
     CDS             475..>702
                     /locus_tag="ACHKV2_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO6943265.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVAPEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGD"
BASE COUNT          169 a          132 c          213 g          188 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgggtgt taaagggata aataattacc cggataagat tactgttact
      541 gtggcaccgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc gattagtctt
      601 gaccgtactg aaggtgccac gctggagttt tacgaagctg aggcgaaaaa gcaggcgaag
      661 cagtttttca tggatgttgc tgccgggtta tgtgaagggg at
//
LOCUS       DAZFTN010000444          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFTN010000444 DAZFTN010000000
VERSION     DAZFTN010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>698
                     /locus_tag="ACHKV2_005629"
     CDS             <1..>698
                     /locus_tag="ACHKV2_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6943266.1"
                     /translation="ISLYPYVISNVFYILPLSILLSFILYFLWQRWISRKMSLAEEIK
                     KGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNISPVVFIRAAEEENLIIPL
                     TKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVADVLHIKNAISDKFNIVLEI
                     TERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGLLQKLSVDYLKIDKSFIDT
                     LTTAEDETPVLDII"
BASE COUNT          210 a          121 c          139 g          228 t
ORIGIN      
        1 tgatttcatt atatccctat gtcattagta atgtatttta cattcttcca ttatctattc
       61 ttctttcttt tatcctgtac ttcttatggc agcgctggat atctcggaaa atgtccctgg
      121 cagaagaaat taagaaagga atgtcatccg gagaattttc tgtgcattat caaccagtat
      181 gtgacacgac aaccaaagcg tgtctggggg ttgaggcgtt aatgcgttgg caacgcgaag
      241 atggtaaaaa tatatctccg gtagttttta tccgcgctgc agaagaggaa aatttaatta
      301 taccgttaac taagcatctc tttgaactga ttattcagga tgtacaaagc tggaaagtga
      361 aaaagccgtt tcaccttggg ataaatattg ccgctgaaca tcttgcacat cctgattttg
      421 ttgctgatgt tttacatata aaaaatgcaa tatctgataa atttaacatt gtccttgaga
      481 ttaccgaacg caatctggtt gaagatactg atcatgcatt acagaaaata aatgaattaa
      541 ggagccatgg ctgtgaattt gcagttgatg attttggcac gggatattgt tcccttggcc
      601 ttctgcaaaa gttgtctgtt gattatctta aaatagataa aagctttata gacacgctca
      661 ctacagcgga agacgaaacg cctgttttag atattatt
//
LOCUS       DAZFTN010000445          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFTN010000445 DAZFTN010000000
VERSION     DAZFTN010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>684
                     /locus_tag="ACHKV2_005630"
     CDS             <1..>684
                     /locus_tag="ACHKV2_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6943267.1"
                     /translation="SKGIHAKYEVDVFIESIRAHIPRNSSLNMNEISEKSLNEEEIKV
                     LFRLLEIGGIENPFHKEVQVRNRLIFTLLLNLGLRAGELLNLKIDDFDLRDNTLSVVR
                     RHDSKEDGRSYQPLVKTGERVIPLSDELAREIFDYISNSREKMTKRKKHNFLFVAHCT
                     GKNAGEPLSISAYEKVISTLKRASPELYNLSGHRLRHSWNYMYSKGIEGAELEYNRRK
                     DLRNYLMGWS"
BASE COUNT          254 a           92 c          148 g          190 t
ORIGIN      
        1 gtcaaagggg attcatgcaa agtatgaagt agacgtattt attgaaagta tcagagcaca
       61 tattccaaga aacagttcat tgaatatgaa tgaaatatct gaaaaatcgt tgaatgaaga
      121 agagatcaaa gtgcttttcc gtctattgga aattggcggg attgaaaacc ctttccataa
      181 ggaagtacag gtcaggaaca gactaatatt taccttgctc ttaaatttag gtttaagggc
      241 tggagagttg ctcaatctta aaatagatga ttttgatctc agagacaaca ctttaagtgt
      301 agtcagaaga catgattcaa aagaagatgg aagatcttat caaccattag taaagactgg
      361 agaaagggtt attccgttat ctgatgagtt agcccgtgaa atttttgatt acataagcaa
      421 tagcagagaa aaaatgacaa aaaggaaaaa gcataatttc ttatttgtgg ctcattgcac
      481 cggaaaaaac gcaggagaac ccttaagcat ttcagcttat gagaaggtga tatcaacact
      541 gaaacgagca agtccagagc tatataatct ttcaggccat aggttaaggc actcttggaa
      601 ttatatgtat tctaaaggaa tagaaggtgc tgaattagaa tataatagaa ggaaagattt
      661 aaggaattat ttaatgggtt ggtc
//
LOCUS       DAZFTN010000446          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFTN010000446 DAZFTN010000000
VERSION     DAZFTN010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /gene="escU"
                     /locus_tag="ACHKV2_005631"
     CDS             <1..>683
                     /gene="escU"
                     /locus_tag="ACHKV2_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO6943268.1"
                     /translation="FAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIIVLI
                     FYFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGL
                     KKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAI
                     CLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARTLYKNIHKGQYIT
                     EDFFEPVAQ"
BASE COUNT          228 a          110 c          130 g          215 t
ORIGIN      
        1 tctttgcggt tgaaaaaata aaaccatcgg ctcagaagat tagtgtaaaa aataacctga
       61 aaaatatttt ttctgtaaag agcatttttg agctacttaa atcagtattt aagttagtga
      121 taattgttct cattttttat tttatggggc attcatatgc aaatgagttt gctaatttca
      181 caggactgaa cgcatatcaa gctcttgtcg ttgttgcctt ttttgttttt cttttatgga
      241 aaggcgtgct attcggatat ctactctttt cagtatttga tttctggttc cagaagcatg
      301 agggactgaa gaaaatgaaa atgagtaaag atgaggtgaa acgagaagcc aaggatactg
      361 atggtaaccc tgaaattaaa ggggagcgcc gtcgccttca ttccgagata caaagtggaa
      421 gtttggctaa taacatcaaa aaatcaaccg ttattgttaa aaacccgact cacattgcga
      481 tttgcctata ctataaactt ggggagactc cattaccttt agttattgaa acaggaaaag
      541 atgccaaagc tctacagatc attaaactgg ctgaactcta tgatattcca gtgattgaag
      601 atattccttt agcaagaact ctctataaga atatacataa aggacaatat ataacagaag
      661 acttttttga acctgtggca caa
//
LOCUS       DAZFTN010000447          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFTN010000447 DAZFTN010000000
VERSION     DAZFTN010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>680
                     /locus_tag="ACHKV2_005632"
     CDS             <1..>680
                     /locus_tag="ACHKV2_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943269.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAW"
BASE COUNT          149 a          171 c          193 g          167 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggtg
//
LOCUS       DAZFTN010000448          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFTN010000448 DAZFTN010000000
VERSION     DAZFTN010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            36..407
                     /gene="ler"
                     /locus_tag="ACHKV2_005633"
     CDS             36..407
                     /gene="ler"
                     /locus_tag="ACHKV2_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO6943270.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            422..640
                     /gene="escE"
                     /locus_tag="ACHKV2_005634"
     CDS             422..640
                     /gene="escE"
                     /locus_tag="ACHKV2_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO6943271.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
BASE COUNT          266 a          101 c          144 g          154 t
ORIGIN      
        1 gcttaaaata ttaaagcatg cggagattat ttattatgaa tatggaaaat aattcacata
       61 caacaagtcc atacattcag cttatagagc aaattgcagt tctacagcag gaagcaaagc
      121 gactgcgaga gcaggaagtt caaagtgtaa ttgagtcgat tcagaagcag attacttatt
      181 acaatataac cttacaagag ctgggatata ctaatgtgcc tgatgatgga ctcgctcgcc
      241 ggaactcatc gaaaggtgtt tactaccgca atgaagaagg gcagacctgg tcgggcgtag
      301 gccgacagcc acgctggctt aaagaagcac tgttgaatgg aatgaagaaa gaagattttc
      361 ttgtgaagga cactgaagaa gaaataatac cgctgaaaaa tatttaacat gaaataatta
      421 aatgataacg ataactgagc tggaagatga aataataaaa aataaagaag ccgcaaatgt
      481 ttttattgaa aaaataaacg acaaaaagaa cgaaatccat gaaaaaatga aacacccttt
      541 ggataaagtt acctacaatg aagcaaagga actgctcatt gcgtgtgatg cggcaattag
      601 gactatagag ataatgcgaa tcaggattaa taataaatag aggatgagta ttgtgagcca
      661 aacaa
//
LOCUS       DAZFTN010000449          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFTN010000449 DAZFTN010000000
VERSION     DAZFTN010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..610
                     /locus_tag="ACHKV2_005635"
     CDS             14..610
                     /locus_tag="ACHKV2_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO6943272.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
BASE COUNT          270 a          121 c          112 g          162 t
ORIGIN      
        1 tataataaaa gccatgctct ggactgaaaa cgatgcggaa aatacttctc agtggaatgg
       61 ttatccatta caaattggtc gttttcggaa agataaagcg atgccagctt taatctctgg
      121 agaaaaatct acagcccttg ttaccccacc acaatggaga aacaaagcat tcaatgggtt
      181 aaaagatcca gaaagaaatt actgggcaaa agaacaaata actggctctc cagaagagaa
      241 tatcaaagca gcaattactt acttaatgat gaaactttct aatacaaagg aagaatcaac
      301 tatcgatcaa tatgactcaa ctctatatag cgccattgtc caaaaaggcg atttggctga
      361 caacatcaga aaagagagaa aaacgaccat acctaactta acgaaaaaca atcctggaaa
      421 aaatctcgat aaaatacacc cgggagatat tttatattat caaaaagcat ccatgaaagt
      481 aataattact ggatggaaac ctatcacaat aaaaaatgta gcgatgaatt ataatggtgg
      541 tggcgatccc aagtatgcaa taaaacttca gttcgtttat acattactca caaaaaacag
      601 agtattatga gaatgcgaaa aataacaatt attctactgc tgacattctc atgtacaaaa
      661 ggtct
//
LOCUS       DAZFTN010000450          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFTN010000450 DAZFTN010000000
VERSION     DAZFTN010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..339
                     /locus_tag="ACHKV2_005636"
     CDS             <1..339
                     /locus_tag="ACHKV2_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6943273.1"
                     /translation="EKGNVTDAEIYYKLLCIYAFENHEYLKGFASVCQSKKKYQQAYD
                     LYKLSYNYSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDASVKSKAQAYIE
                     LLTDNSEDNG"
BASE COUNT          243 a          100 c          105 g          200 t
ORIGIN      
        1 gaaaagggta atgtaacgga cgcagaaatt tattataaat tattgtgcat ttatgcgttc
       61 gaaaatcatg aatatctaaa aggttttgca tcagtatgtc agtcaaaaaa gaaatatcaa
      121 caagcatatg acctttacaa gctaagttac aattactccc cgtatgatga ctattcagtt
      181 atttatcgta tgggtcaatg tcaaattggg gctaaaaata tcgataacgc aatgcaatgt
      241 ttctatcata ttattaacaa ttgtgaggat gctagtgtta agagtaaagc gcaggcatat
      301 attgaactct taaccgataa ttcagaagat aatggctaag cattacaatc atttggttaa
      361 aaatgctggt tatagtgtag taaccactat gaccagcacc tcttatgctt tcttaaatca
      421 acatcttaaa acagttaaga acacactaaa aaatattatt tttcaacgcg ttagagaaca
      481 atctatttta tcaaaattga ataaggctat ttcagattac atcagtgaat tcctaagata
      541 aattagtgaa cacaaccata gatatgtcga atgtaaactc ctgtagcgtt aatgacacat
      601 aaatatgaat agccataatt tctattgctt atgcaggatg caagaaac
//
LOCUS       DAZFTN010000451          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFTN010000451 DAZFTN010000000
VERSION     DAZFTN010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..328)
                     /locus_tag="ACHKV2_005637"
     CDS             complement(<1..328)
                     /locus_tag="ACHKV2_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943274.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKN"
BASE COUNT          202 a          114 c          103 g          224 t
ORIGIN      
        1 catttttaac accacctctt ggtttcacat caatttttaa aagtgatata accggagcag
       61 gtggcgaatt atgttttctt tcgtcatttg aagtatgatt gtttttatat cttggatcat
      121 gaataccaca tccatcctta tgtttatcgc catatctgaa atatgattga ttaacatgtt
      181 tcatagtaaa actacaaggt gtggctttaa ttttacaagg ctcgtatggg cattcaaaac
      241 tcgttgtctt aatgtaaaaa tcatccattc cacggagatc atttccatga atgatttcat
      301 cagtcgaact atccttcgca atcgtcatat cttaacccta tgaaaatcta tgtccttcta
      361 aggattctag ctttttctta ttgacgatcc aatcaaaaaa ttgtactcca acttaccaaa
      421 atacacgatc aagcagaagt tattaactta aggggtagaa ttagtatttt tcaaagaaat
      481 taaatctttg acagaatgat gctgaataac atcgctaacc ttagtaaggt cagggagttg
      541 ttatacacaa ctcccctcta ttatcgtttg ttaaaggttc tgggtatgta tgcatggtaa
      601 cagtaaaagc atcttagagg atgcttttaa gcattaatta gat
//
LOCUS       DAZFTN010000452          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFTN010000452 DAZFTN010000000
VERSION     DAZFTN010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..161)
                     /locus_tag="ACHKV2_005638"
     CDS             complement(<1..161)
                     /locus_tag="ACHKV2_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6943275.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..567)
                     /locus_tag="ACHKV2_005639"
     CDS             complement(142..567)
                     /locus_tag="ACHKV2_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6943276.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          143 a          211 c          145 g          143 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccggaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccgactc agtcgcttct gctttaccgg tt
//
LOCUS       DAZFTN010000453          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFTN010000453 DAZFTN010000000
VERSION     DAZFTN010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHKV2_005640"
     CDS             complement(<1..194)
                     /locus_tag="ACHKV2_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943277.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
     gene            complement(244..546)
                     /locus_tag="ACHKV2_005641"
                     /pseudo
     CDS             complement(244..546)
                     /locus_tag="ACHKV2_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          138 a          204 c          141 g          150 t
ORIGIN      
        1 gccagcgcct gtcggctcag actctgctca gcagcatcag cctgttttct gaaagactga
       61 ctgactttcc cggacatcaa atcagcaagc gcactggttt cacccagctc tttttttacc
      121 cgcgctgcct cttcagaaaa acgggcagaa tccagtgtca gcacggctgt cagatcggca
      181 aaattacctg ccatcgtggt cacctcctga aatgtcctct gataccatca ataactgtca
      241 caacctcctt ccctcctccc cgaaacggac tccaccggcg aggcccgccg ccttctgcat
      301 cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc tctgcctccg gagcgaacag
      361 gctgaaatcc gccggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacat
      421 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc agtaaaaacg
      481 tcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcggctct
      541 ccccatctca cgcgccagtt tcaggacaaa gttcagctcg ccttcgaatg cttttttgat
      601 gttaccggct cagtcgcttc tgttttcccg gtt
//
LOCUS       DAZFTN010000454          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFTN010000454 DAZFTN010000000
VERSION     DAZFTN010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(14..343)
                     /locus_tag="ACHKV2_005642"
     CDS             complement(14..343)
                     /locus_tag="ACHKV2_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6943278.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(340..>624)
                     /locus_tag="ACHKV2_005643"
     CDS             complement(340..>624)
                     /locus_tag="ACHKV2_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6943279.1"
                     /translation="KEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQ
                     NNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          126 a          232 c          131 g          135 t
ORIGIN      
        1 tgaatatcct gcatcagttc accacctgct taaactccgc gctgaactca acgcgcaaca
       61 tcccgacccg cgcagaccac ccggcacagg tcacctttat ctgccggtat gcatagggtg
      121 gcttccacaa aaatgccttc cagcctccgt gctctgccag gaacgcttcc agatgccggg
      181 cctcctcccg ggtcacggaa agcgtcacac ggtatgtttt caggtcagca ttcagccctg
      241 ccgccatacg ctgtgagtac ccgtcaccaa aacgcacttc acgcaccgat ggctgcgagt
      301 tcacctccat atccggcttc actttccagc gaaaggtttt catcgcccgc tccccgataa
      361 cataccgcca tcacgcaact gcagccggag ctcatcctgc gcccccttgc gggccatgtc
      421 atacaccgcc ttcatcagct gcggccccgc ctgtccgttg gtgccgtcgt tctgaatcac
      481 cacgtgattg ttctgattaa aattaatacc ttccgcccgc cgcatctgcg ccggacttcc
      541 ggcaccaccc acataaccac cttccgcata gccgcgcatc agacggtaaa gattccccac
      601 acctatccgg ctggttgcct cttt
//
LOCUS       DAZFTN010000455          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFTN010000455 DAZFTN010000000
VERSION     DAZFTN010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>623
                     /locus_tag="ACHKV2_005644"
     CDS             <1..>623
                     /locus_tag="ACHKV2_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO6943280.1"
                     /translation="NYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYL
                     LKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVD
                     ESGQVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLA
                     VDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLI"
BASE COUNT          190 a          119 c          137 g          177 t
ORIGIN      
        1 aactatcaac tggtatcgga acgcgcggta aaacgtgccg atagccgctt tgaacttatt
       61 cagaaaaacg ttggctattt ctttaaagat attgaacgtt cggccctgac attaaaggac
      121 tcactgtatt tattaaaaaa tacagaggag attcaacgcg ctgtgatcct gaaaatggaa
      181 atgatgccat ttttagactc ggtgggactg gtacttgatg ataataaata ttatctcttt
      241 tcgcggaggg cgaacgataa aatcgttgtt tatcatcagg aacaagtaaa tggaccgctt
      301 gtcgacgagt cagggcaggt tatttttgcc gattttaacc catcgaaacg accgtggtcg
      361 gtggcttcag atgactctaa caacagctgg aatccggcct acaattgctt tgatcgtccg
      421 ggtaaaaaat gtatctcttt tacgctacgc atcaacggca aagatcacga tttgttagcg
      481 gtggataaaa ttcatgtcga tttaaactgg cgatatctga acgagtatct tgatcaaatc
      541 agcgctaatg atgaagttct atttttgaaa caaggccatg agatcattgc caagaatcaa
      601 ctcgctcgtg aaaaactgat tat
//
LOCUS       DAZFTN010000456          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFTN010000456 DAZFTN010000000
VERSION     DAZFTN010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>619
                     /locus_tag="ACHKV2_005645"
     CDS             <1..>619
                     /locus_tag="ACHKV2_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO6943281.1"
                     /translation="LDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDR
                     LTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPET
                     GELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRL
                     HRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNG"
BASE COUNT          147 a          177 c          206 g           89 t
ORIGIN      
        1 cgcttgaccg gctggtacag cagcgcggct ttgacgggcg gacgcaacgt taccactatg
       61 acctgaccgg aaaactcacg cagagtgaag atgaggggct tgtcaccctc tggcactacg
      121 acgaatcgga ccgcctcact caccgcacgg tgaacggcga accggcagag cagtggcagt
      181 acgacgagca cggctggctg acagaaatca gccacctgag cgaaggccat caggtggcgg
      241 tgcattacgg ttatgatgat aagggccgcc tggccgggga gcgccagacg gtgcataacc
      301 cggagacggg ggaactgctg tggcagcatg agacagagca cgcatacaac gaacagggtc
      361 tggcaaaccg cgtcacgccg gacagcctgc cgcgggtgga gtggctgacc tacggcagcg
      421 gttatcttgc ggggatgaag ctgggcggga cgccgctggt ggagttcacg cgcgacaggc
      481 tgcaccgcga gacggtgcgc agcttcggca ataacgcata cgaactgacc agcacataca
      541 ctcccgcagg ccatttacag agccagcgcc tgaacagcca ggtgtatgac cgtgactacg
      601 actggaatga caatggcga
//
LOCUS       DAZFTN010000457          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFTN010000457 DAZFTN010000000
VERSION     DAZFTN010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..126
                     /locus_tag="ACHKV2_005646"
     CDS             <1..126
                     /locus_tag="ACHKV2_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943282.1"
                     /translation="AVAEMFHNAAWGVRTLWFELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(226..441)
                     /locus_tag="ACHKV2_005647"
                     /pseudo
     CDS             complement(226..441)
                     /locus_tag="ACHKV2_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(485..>616)
                     /locus_tag="ACHKV2_005648"
     CDS             complement(485..>616)
                     /locus_tag="ACHKV2_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6943283.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          166 a          148 c          142 g          160 t
ORIGIN      
        1 gccgttgcgg aaatgtttca taacgcagca tggggagtgc gaacattgtg gtttgaactc
       61 gtcacaaaaa tagatataga tatacataaa aagaacagat atgcgagtta tgatcttgat
      121 cgataggaat ttaaatccca aaaagattaa aaaaacaccc aaaaacggat gtttattcaa
      181 cactgcctgc gtgccatgag tgcaccgccg tcaattaaat ggatctattt catcctgacc
      241 agcatagtgc tccagacggc tcatcggcac cggcggtttt ttcaggtaag agccgaagta
      301 caccgccaca tgtgtggcgt tatccatcac ccgggatacg ttgacattcc agccccgccg
      361 gtaatgactg tccaggaagc gattccagtc ccgtttactg cttccttctg ctgccagcgc
      421 atccggtatc accaggtccg gtgaggtgta ctggcaatag cggacactac catttgttct
      481 ttttttaagc agccatctga tgatattttt ccctgaaggc tgccggggag atattcccca
      541 gacgagagtg acgacgctga cgattgtaga aaatctcaat gtattcccgt attactgaga
      601 tggcttcatc ccggtt
//
LOCUS       DAZFTN010000458          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFTN010000458 DAZFTN010000000
VERSION     DAZFTN010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..531)
                     /gene="fimE"
                     /locus_tag="ACHKV2_005649"
     CDS             complement(<1..531)
                     /gene="fimE"
                     /locus_tag="ACHKV2_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO6943284.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAA"
BASE COUNT          182 a          156 c          129 g          147 t
ORIGIN      
        1 agcagcatta ctggcggtat aacgtacagt atggcgaata tttcgatgcc cgagataatc
       61 ctgaattaaa cgagtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
      121 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcac gaataatgcg
      181 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatgg cgtcggtccg
      241 gtcagcgcct ttccagttag cacgttctag ggtccagcgt tccacagctt cacgctcatc
      301 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcggcc
      361 ttcattaagg tcaagatcct gataatgcag atcaagcagt tcactaatac gcatcccatg
      421 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
      481 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
      541 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
      601 gcccaataga taaa
//
LOCUS       DAZFTN010000459          610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFTN010000459 DAZFTN010000000
VERSION     DAZFTN010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>610
                     /locus_tag="ACHKV2_005650"
     CDS             <1..>610
                     /locus_tag="ACHKV2_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943285.1"
                     /translation="HFYDYSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNND
                     FTEESFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHY
                     NDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLINLKTLLNDGCFI
                     KYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENGSLGDV"
BASE COUNT          203 a           86 c          118 g          203 t
ORIGIN      
        1 ttcattttta tgattatagc aggcgatatg caattaatga aaatattgca aaagacgctg
       61 taacaaacaa tatcgcattg atgagcactt tttatgacag tgataatacg tcaggtgagg
      121 ttttaaataa tgattttaca gaagagtcat ttgaaactgc gttagatgta gagcatacaa
      181 tagtgcttgg atttgctgat gataattttg aaacaaaacc tgttcattat caggatttat
      241 taacacgatt tagtgccttt caagataccg tgtttaatct gtttcctgaa atgcactctt
      301 cgcattacaa cgatatttgt agcgtaagtg tggatatgac aaagggaacg caatgcatga
      361 ttcacttgat ggtgaatgaa gaagttttca tgtctctgcc tgttccagtt gccacgatgg
      421 tacgtgaaga tgcaagtaat ttaatcaatc ttaaaacact actcaatgat ggttgcttta
      481 ttaaatatag tcattttaat gacgtggcat taataaagca aaacataagt aatctctatt
      541 tatcacacac tgttgtgaat gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt
      601 taggtgatgt
//
LOCUS       DAZFTN010000460          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFTN010000460 DAZFTN010000000
VERSION     DAZFTN010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          208 a          104 c          123 g          173 t
ORIGIN      
        1 ctgaaacaga gaggttcaag ataaagtatc aacaagagaa tacacttaat gatgtagttc
       61 aaaatatcag gacttggcta aaagaaaatc aaaatatggt gaaagctatt aagaaaattg
      121 atactgaata acatggcgaa taaataaacg gatcaagtcg ttcctatgac aatgaggtta
      181 tacgaaaaga cgcttactca tcagaaatct ggtatgcttt aattaaacca gtgtcgctga
      241 tgtgtaagcg ttgacgttgg gagacgatta aatggttata ccactcctac gtcttttcca
      301 tacggcgtat aagagtgcaa catgttgagt tttccgagtg aatggtgcat tttactaacc
      361 gagccatttg aatcaaccaa cgcaataaaa aagactggaa taataagcct gtatttatta
      421 tttatcgcaa tcgcacttta cggttttatc caactgaaaa gagactgctt ccagcggttg
      481 tggattatcc ctgggcgata gtcatagtct ggtgtcagga ttcacaaatg acctgtttcc
      541 ttacatggat ataaacacaa catagagtgc ggtagttaat ctgaataatc taatttcggc
      601 tattcagc
//
LOCUS       DAZFTN010000461          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFTN010000461 DAZFTN010000000
VERSION     DAZFTN010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>603
                     /locus_tag="ACHKV2_005651"
     CDS             <1..>603
                     /locus_tag="ACHKV2_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6943286.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHC"
BASE COUNT          136 a          176 c          199 g           92 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cga
//
LOCUS       DAZFTN010000462          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFTN010000462 DAZFTN010000000
VERSION     DAZFTN010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          193 a          128 c          127 g          153 t
ORIGIN      
        1 aattcgagtt acaggaaggc ggcaagcgag tgaatcccca ggagcttaca taagtaagtg
       61 attggggtaa gcgaacgcag tcgcagcaca ttcaacttga agtatgacga gtatatatat
      121 ctattagctg tttcccggac ataagctaca gccagcacta acctggaacg ggtgcattat
      181 aaaaaggaac aagggacaaa tagctgaaca gtaaccgcgt tagtctcttc ccgcataacc
      241 gtaccattcg tattaagaag tattgcccat atccattcgg cgaaagagga tctgccatac
      301 atcaagtccg ttttacttgg catcagatca tcgccgtcat taactctgtt aaagtcggac
      361 aaacgattag caagatctgt gattcaaaag tgcttagcgc taaggtggga ctggttacag
      421 attcattaac cctgttcttt gtcgcaccta tcgatcggtg taatgccaca acaggtttat
      481 gttaatcaga acaatgcgcc cctctataaa gagaattctt tgataaggca acttgaaaag
      541 ggaaagctaa ctgatatcaa tgtaccactt tagtaatcat cacattatat acaggccaaa
      601 a
//
LOCUS       DAZFTN010000463          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFTN010000463 DAZFTN010000000
VERSION     DAZFTN010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..532
                     /locus_tag="ACHKV2_005652"
     CDS             2..532
                     /locus_tag="ACHKV2_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6943287.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
BASE COUNT          168 a          125 c          162 g          143 t
ORIGIN      
        1 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatacattc cgcctcatac
       61 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggc
      121 tgttttcaac agtaatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
      181 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa
      241 tgttacctgg ttgtcgccgg atggagagtg tcagaagtgg aacggcacat cctgggcgaa
      301 agatgcagaa gcagaaaaac tgtttcgggt acgggaggca gaagaaacaa aaaacagcct
      361 gatgcaggta gccagtgagc atattgcgcc acttcaggat gccgtagatt tggatattgc
      421 gacggaggaa gaggcatcgt tactggctgc atggaagaca tatcgggtat tgttgaatcg
      481 tgttgataca acagtagcag cggatgttga gtggccagtc gccccacaat aaaaagaaaa
      541 agccatcgac agaaatatcg atggctttat gtactctatt tatacaatac aacaccac
//
LOCUS       DAZFTN010000464          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFTN010000464 DAZFTN010000000
VERSION     DAZFTN010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..581
                     /locus_tag="ACHKV2_005653"
                     /pseudo
     CDS             61..581
                     /locus_tag="ACHKV2_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448642.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          163 a          101 c          136 g          181 t
ORIGIN      
        1 ggcagatgtt tgttaaagct atttaagtct ggagtttaaa ttaaaatagg gagttttata
       61 atgccgttaa attcggagat tagatcaagc tcatttttaa tggattgaat gtccttcgag
      121 ctcaagtagc atctagcggt cgaggggagt ttacattagg taatgagact gtcagcattg
      181 tatttaatga aaccgatggg cgttttctat ccagcggcag tagtggggga ttgcttactg
      241 agttattcct ttatgggttt aataacggcc ctgaagctct tcgcgatagg atgctcagta
      301 tgctttcgga ctcaggtgaa gcacaatcgc aagagagtat tcaggacaaa atatctcaat
      361 gtaagtttcc tgttagttca ggaaatttcc agtgcccgcc agagtctatt cagtgtccaa
      421 ttacactaga gagacccgaa gaaggagtgt ttgtcaaaaa ttcagatagt tcggcagtat
      481 gctgcttatt tgattttgat gcattttctc gtttagctag tgaaggctca tatcatccac
      541 tgacccgaga accaataacg gcatcaatga ttataagtcc t
//
LOCUS       DAZFTN010000465          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFTN010000465 DAZFTN010000000
VERSION     DAZFTN010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..214
                     /locus_tag="ACHKV2_005654"
     CDS             <1..214
                     /locus_tag="ACHKV2_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943288.1"
                     /translation="SLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSPII
                     PLNWSKASMTLMLKEMSNLINDEGIK"
     gene            281..460
                     /locus_tag="ACHKV2_005655"
     CDS             281..460
                     /locus_tag="ACHKV2_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO6943289.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT          205 a          100 c          118 g          155 t
ORIGIN      
        1 atcattggcc caaataacag gcgagaatat gattggtgca aatggcagga gcgtaccaga
       61 aatggcactt cccgaaagct ataactatat ccacaagtcg ggaactttgc atgaagcgcc
      121 aagccctatc attccgctaa attggtcgaa agcttcaatg actctcatgt tgaaagagat
      181 gtccaacctt atcaatgatg agggaattaa ataattttca taatgaggcg gtcctggatt
      241 aaaaggaccg ctcaaagaga aatcgaaagg ggaggtaaaa gtgatcaata aattactgtt
      301 agcttacctg ataggtctgg ttgtgacatc gactttaata tttatcttct cggaagaaaa
      361 agtaacatat cggttgtttg ctgcaataat tacaggttta acctggccgc ttagtctcat
      421 tccttccatt ataagtctca tgattcgtaa atccgactaa caagaaaatt atatgatcga
      481 ttaagaaaaa tagaatcgaa caagagaata aaatatatgc ggggagtaac aaaacactcg
      541 cagtgtgtta atgaagaact aacaaatatg atttgtat
//
LOCUS       DAZFTN010000466          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFTN010000466 DAZFTN010000000
VERSION     DAZFTN010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>576
                     /gene="espR1"
                     /locus_tag="ACHKV2_005656"
     CDS             <1..>576
                     /gene="espR1"
                     /locus_tag="ACHKV2_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO6943290.1"
                     /translation="SIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIHRPEC
                     RNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNYDRLV
                     KLSLNSNTLESINIHQGRNVSITHISMNNNCLRNIDIDRLSSITYFSAAHNKLEFVQL
                     ESCEWLQYLNLSHNQLTDIVTGNKEELLLLDL"
BASE COUNT          222 a          108 c           85 g          161 t
ORIGIN      
        1 tcaatattta acaaaataaa accacaatcc atacagcaac atccagaaaa aaatcaactt
       61 aactggatgc tcgaattaaa taaatggaaa gaagaacgta tacttacagg tgaaatccat
      121 cgtccggaat gtcgaaacga agccgctaaa aggataaact gtgctttttt gtcgaaacag
      181 aatgacattg atttatcagg acttaattta tctactcaac caccagggct gcaaaacttc
      241 acctctatca atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga
      301 ctcgtaaaac ttagtctgaa tagtaacact cttgagtcaa taaatattca tcaaggcaga
      361 aatgtaagca ttacacatat atctatgaat aataattgtc tcagaaatat tgatatagat
      421 aggctttcat caattactta ttttagtgcg gcacataata aactagagtt tgtgcaatta
      481 gaatcttgcg aatggctgca atacctgaat ctcagccata atcaattaac tgatattgtt
      541 acaggaaata aagaagaact cttactgctg gatcta
//
LOCUS       DAZFTN010000467          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFTN010000467 DAZFTN010000000
VERSION     DAZFTN010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHKV2_005657"
     CDS             complement(<1..203)
                     /locus_tag="ACHKV2_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6943291.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..>571)
                     /locus_tag="ACHKV2_005658"
     CDS             complement(355..>571)
                     /locus_tag="ACHKV2_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6943292.1"
                     /translation="GDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTESV
                     TIDLAYEGSGSGDWRSDAFIVGIGYRF"
BASE COUNT          141 a          167 c          123 g          140 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgcacgccag ccccccacgc
      481 cagagacgtg ttgctgtggc gaccgtcatc acttccggtc agcacatcgt gcgttttccc
      541 cttgttgtca gttacgcgga gataatcccc g
//
LOCUS       DAZFTN010000468          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFTN010000468 DAZFTN010000000
VERSION     DAZFTN010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            41..325
                     /locus_tag="ACHKV2_005659"
     CDS             41..325
                     /locus_tag="ACHKV2_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO6943293.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            325..>550
                     /locus_tag="ACHKV2_005660"
     CDS             325..>550
                     /locus_tag="ACHKV2_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6943294.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELN"
BASE COUNT          146 a          131 c          146 g          127 t
ORIGIN      
        1 gtaatcacta tgattaaata tgattaacga ctaatcggat atgcaaatga aaaacaatac
       61 cgcacaagca acaaaagtca ttaccgcgca tgtgccatta cctatggctg ataaagtcga
      121 ccagatggcc gccagactgg aacgctcccg gggttgggtt atcaaacagg cgctttctgc
      181 atggcttgcc caggaggagg agcgtaatcg cctgacgctg gaagccctgg acgatgtgac
      241 atccggacag gttattgacc atcaggctgt acaggcctgg gcggacagcc tcagtactga
      301 caatccgtta ccggtgccac gctgatggaa ctgaagtgga gcagtaaagc gctttctgat
      361 ttggcgcggt tatatgattt tctggtgcta gccagtaaac ctgctgccgc cagaacggta
      421 cagtccctga cacaggcacc ggtcattctg ttaactcatc cacgtatggg ggaacagtta
      481 tttcagtttg aacccaggga ggtcagacgg atttttgctg gcgagtacga aatccgttac
      541 gaacttaatg
//
LOCUS       DAZFTN010000469          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFTN010000469 DAZFTN010000000
VERSION     DAZFTN010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..496
                     /gene="espW"
                     /locus_tag="ACHKV2_005661"
     CDS             <1..496
                     /gene="espW"
                     /locus_tag="ACHKV2_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO6943295.1"
                     /translation="AKPNLGERDVRMFENISSDDTDKITGRKFSELGEYLYSGNVIKL
                     SQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNIKT
                     MLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTWDP
                     LVRN"
BASE COUNT          211 a          101 c           95 g          143 t
ORIGIN      
        1 tgcaaaacca aacttaggag agcgagacgt aagaatgttc gaaaatatta gttctgatga
       61 tacagataag ataacaggaa ggaaattttc agagttaggt gagtatcttt attcagggaa
      121 tgtaataaaa ctcagtcagt tatcaattcg ttacttaccc aatatcagct caatctcatt
      181 aatagagaca aaacagagtt tgttgctaca tcgattgtat tcagatgaag tacttcagag
      241 aaatggaacg cttatcccga caccactaca tgaagaaaaa tcaattccag ctgacaatat
      301 aaaaacaatg ctcaacaaca taccgactta caaaatgtta ccgccattca cagaaacaca
      361 aggtaattgt tcttctggcg cagccacgtt tttacgcaaa tcaggcgccg aagaaaaaga
      421 tattcttgca tgtagccccc gaaattatgg gctgcatcat aacataaaaa catgggaccc
      481 cttggttaga aattaaggat ccaaatatta tatatttcat ataaagcaag aaaaaaatat
      541 tgtctgaata
//
LOCUS       DAZFTN010000470          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFTN010000470 DAZFTN010000000
VERSION     DAZFTN010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            278..>537
                     /locus_tag="ACHKV2_005662"
     CDS             278..>537
                     /locus_tag="ACHKV2_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943296.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQL"
BASE COUNT          148 a          101 c          149 g          139 t
ORIGIN      
        1 gatggcgtag atgcacgttg tgacatgtag atgataatta ttatcatttt gcgggtcctt
       61 tccggcgatc cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat
      121 tttccgggat ccatgtccgg tttctcttca agttaactat atgaaaaata taaaaacagg
      181 tcttctgtga accggacatg aacaaaaaac agacatgtaa accggacatg accggttttg
      241 ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg gctacgcaga ctgaagttgc
      301 caggcattta agtctgaccg atcgccagct tcgcagattg cagaaattgc cgggtgcccc
      361 gatatcgaat aagcgagggc aactggatct ggatgcctgg cgcgattttt acatatcgta
      421 tctgaggaga agtaaaaacg atgtgcctga tggcgatagc gaagacgact atgaggagaa
      481 attgcttatt gccagatggg aactgacagc agaacaggct gttacacagc agttaaa
//
LOCUS       DAZFTN010000471          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFTN010000471 DAZFTN010000000
VERSION     DAZFTN010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>530
                     /locus_tag="ACHKV2_005663"
     rRNA            <1..>530
                     /locus_tag="ACHKV2_005663"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          120 c          167 g          100 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt
//
LOCUS       DAZFTN010000472          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFTN010000472 DAZFTN010000000
VERSION     DAZFTN010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>530)
                     /locus_tag="ACHKV2_005664"
     CDS             complement(<1..>530)
                     /locus_tag="ACHKV2_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943297.1"
                     /translation="KPVVDYYINNSNHNEQLSIPPYGTRSYKDTFQLIFYKNNIRYYK
                     PAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEIDVSDWNKSQKDVFWKEYE
                     KQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFLFKTNHFKLVYVAFLGKFESS
                     YNDSNYYIENDLSVSS"
BASE COUNT          168 a           93 c           77 g          192 t
ORIGIN      
        1 atgatgatac agacagatca ttttcaatgt aataattact atcattatat gagctttcaa
       61 atttacccaa gaaagccaca tatactaatt taaagtggtt tgtcttaaat agaaacttat
      121 cattttcatc cggcatccca aaaaagaaaa ccgtcaagta ttttttagca ttccttttat
      181 ctttaagccc cttataatac ctatcagctt ccttcctttg cttttcatat tccttccaaa
      241 acacatcctt ctgactttta ttccagtcag aaacatcaat ttcaagcgta aatggttttt
      301 gggttttctt atcccttgcg aggaaagtaa tacaatgttt atctgaatct tcatgcagac
      361 gaatattaga ctgggctaca ccataataaa tagcaggttt gtaataacgt atgttgtttt
      421 tgtaaaaaat aagttgaaat gtatctttat aagatcgcgt gccatatgga gggattgata
      481 gctgttcatt atgattgcta ttatttatgt aatagtcaac gactggctta
//
LOCUS       DAZFTN010000473          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFTN010000473 DAZFTN010000000
VERSION     DAZFTN010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>528)
                     /gene="espX2"
                     /locus_tag="ACHKV2_005665"
     CDS             complement(<1..>528)
                     /gene="espX2"
                     /locus_tag="ACHKV2_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO6943298.1"
                     /translation="KILRMKIELTEGSGFSKSLSDSQIEMHILRTVNSLNVGEKLNDD
                     NLADHSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSKVKDIVDDMSNHDVES
                     EKDIFVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGMELFALRDIVAFPSTI
                     IAKLIKSPLNSDHELV"
BASE COUNT          157 a          104 c           83 g          184 t
ORIGIN      
        1 acaagttcat gatcgctatt aagtggggat tttattaatt ttgctattat ggttgatggg
       61 aatgccacta tgtcccgcag cgcgaagagc tccatgccat cagttaattg cgttaacaga
      121 tcttttactg tttgtatgtt atcagtacta tcattgaaag attcgtctgc cagagaattc
      181 agtttttcta tcatcccccc gattacaaat atatcttttt cgctctccac gtcatgatta
      241 gacatatcat cgacgatatc tttaacttta ctatcacttt caaatagttt ttctctcaca
      301 atattattag cgtttactgc agaagttgca tcataatagg atggcggaat aataacagag
      361 aattcgttag tgaatataga atgatctgca agattatcat cattcaattt ctcgccaaca
      421 tttagcgaat taacagtcct cagaatatgc atctcaatct gactgtcaga aagactttta
      481 gaaaaaccac taccttcagt cagttcaatt ttcatcctga gtatttta
//
LOCUS       DAZFTN010000474          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFTN010000474 DAZFTN010000000
VERSION     DAZFTN010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..425
                     /locus_tag="ACHKV2_005666"
     CDS             <1..425
                     /locus_tag="ACHKV2_005666"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO6943299.1"
                     /translation="NCMNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALK
                     ACIAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKD
                     WSEQFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
BASE COUNT          171 a           79 c          105 g          160 t
ORIGIN      
        1 ataattgtat gaataaaaac gaccttgaag cattatctga cactaggctt aatgaggcca
       61 aatgcttgct tgatcatggc ttttttcatg gtgcatatta tctttgtggg tatgcagttg
      121 aatgtgcatt gaaggcctgt attgccaagt catttttaca acatgagttt ccaaacaaaa
      181 aagtcgtaaa tgattcatat actcatgatt tgtcgcaact tctcaaaatt gccaacttac
      241 atcaaatttt gattgctgac gcaaaaaatg atgtttcgtt ggagattaac tggtcggtcg
      301 ttaaagactg gagcgagcaa tttagatacg acaataacat aagtaaagct atggccgaac
      361 aattgtttga tgctgtaggt gaccaaaatt ctggagtttt gaaatgggta aaagcacact
      421 ggtaatcggc agagagttga caaaagatat ggaattctca ggtcaatttt tgttaaaaaa
      481 actcaagtta cagaatttaa ctattgatgc tgcaa
//
LOCUS       DAZFTN010000475          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFTN010000475 DAZFTN010000000
VERSION     DAZFTN010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>506
                     /locus_tag="ACHKV2_005667"
     CDS             <1..>506
                     /locus_tag="ACHKV2_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6943300.1"
                     /translation="ALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTYFWKQKN
                     EPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGSTWRNSA
                     ATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVPSPSETE
                     AVTTTLLS"
BASE COUNT          140 a          128 c          127 g          111 t
ORIGIN      
        1 gcgctgtttc tggcgatggt ggaaaaattc cgcgcagacc tggctgaaca ggcggaacag
       61 tgtaccggtg gcagtgctgc tggcgttccg tggatttgtg gcgataccac gtatttctgg
      121 aaacagaaaa atgaaccagc ataccaggca atctatggcg gctacaaaaa caaaacagat
      181 aaaaacattc atttcgttcc attaatgaca gatgaaaatg gcgtgaatgt acccaccaac
      241 aatccggcag aagacccgga tattgaatcc attggttatt acggctcaac gtggcgtaac
      301 agtgccgcta cctggacatc tgcggaccgg gccagccatt tcagttcatg ggcacgtcgt
      361 gggattattt ccgaccgtct ggccacggca gttctgactc atgcggggcg aaccacagta
      421 aaagccgatg ttccatcttc tgaaactgaa gcaccagtgc cgtcaccttc agaaactgaa
      481 gcagtaacca caacactgct gtctta
//
LOCUS       DAZFTN010000476          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFTN010000476 DAZFTN010000000
VERSION     DAZFTN010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>506
                     /locus_tag="ACHKV2_005668"
     CDS             <1..>506
                     /locus_tag="ACHKV2_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6943301.1"
                     /translation="ALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTYFWKQKN
                     EPAYQAIYGGYKNKTDKNIHFVPLMTDENDANVPTNNPAEDPDIESIGYYGSTWRNSA
                     ATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVPSPSETE
                     AVTTTLLS"
BASE COUNT          138 a          131 c          128 g          109 t
ORIGIN      
        1 gcgctgtttc tggcgatggt ggaaaaattc cgcgcagacc tggctgaaca ggcggaacag
       61 tgtaccggtg gcagtgctgc tggcgttccg tggatttgtg gcgataccac gtatttctgg
      121 aaacagaaaa atgaaccggc ataccaggca atctatggcg gctacaaaaa caaaacagat
      181 aaaaacattc atttcgttcc attaatgaca gatgaaaatg acgcgaatgt acccaccaac
      241 aatccggcag aagacccgga tattgaatcc attggttatt acggctcaac gtggcgtaac
      301 agtgccgcca cctggacatc tgcggaccgg gccagccatt tcagtgcctg ggcgcgccgg
      361 ggtattattt ctgaccgtct ggccacggct gttctgactc atgcagggcg aaccacagta
      421 aaagccgatg ttccatcttc tgaaactgaa gcaccagtgc cgtcaccttc agaaactgaa
      481 gcagtaacca caacactgct gtctta
//
LOCUS       DAZFTN010000477          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFTN010000477 DAZFTN010000000
VERSION     DAZFTN010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..302
                     /locus_tag="ACHKV2_005669"
     CDS             <1..302
                     /locus_tag="ACHKV2_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO6943302.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHKV2_005670"
     CDS             299..>504
                     /locus_tag="ACHKV2_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO6943303.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT          114 a          117 c          169 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcagggac
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZFTN010000478          495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFTN010000478 DAZFTN010000000
VERSION     DAZFTN010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHKV2_005671"
     CDS             <1..375
                     /locus_tag="ACHKV2_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6943304.1"
                     /translation="KADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRV
                     NDISGLAFNNESLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVT
                     LPDESIYVASEPDILAKFVPNK"
BASE COUNT          166 a           96 c           89 g          144 t
ORIGIN      
        1 aaagcagatg atcggatgct gtttgtcgca aaagaaagac gcccaagtaa aatatttata
       61 tatcaacttt ctccagacct gctgagtgta aagcaagcaa ctattccaga ggcactaaat
      121 gatatacgtg ttaacgatat atccggcctg gcctttaata atgaaagtct gatgattctg
      181 tccgatgaat cgcgtaaatt actcaagttt aacctgacag agatgtcttt cattgagatg
      241 cttgacctga caaaaggaaa tcattctctg accagtgatt tgccccaacc tgaaggaatt
      301 gtaactctgc ctgatgaaag catttatgtt gccagtgaac ctgatatcct cgcgaaattt
      361 gtccctaata agtaagtctg tcactggcct tattatttat cgaccaaacc tggcaatctc
      421 ataaagttat aaaaatacga gtataatttc acgctaaata aacacattaa tagttgaaaa
      481 ggatatcagt tatta
//
LOCUS       DAZFTN010000479          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFTN010000479 DAZFTN010000000
VERSION     DAZFTN010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>481
                     /locus_tag="ACHKV2_005672"
     CDS             <1..>481
                     /locus_tag="ACHKV2_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO6943305.1"
                     /translation="LIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLV
                     AHGANNFYESLSPLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGG
                     YSGMKIVPKYNRLIRPSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKIIQISSTVKKF
                     "
BASE COUNT          160 a           72 c           94 g          155 t
ORIGIN      
        1 ttgataaaat atgtgaactt aagtttcgat attgttgcat ctgtaggaca ggtcgtaggc
       61 ggggttggcg cgttgaaatt tggtaaggta gtgcattcaa accgaataaa aggtataggc
      121 gtcacattag ttgctcatgg ggcgaataat ttttatgaaa gcttatcacc tttatttttt
      181 aacgagtatg attcaggtcc aatacgtgaa ctctacagag taatagcaaa aaaaatggga
      241 ggtgatatta attctgctga ttatgcatac agcatcgtgg acttttcgat tactgcatat
      301 ggtggatatt caggcatgaa aatcgtacct aagtataata gattgattcg tccttcatta
      361 ggaaatagac ctggtacagg acgattattt cattacacat cagttgattt taaaaataaa
      421 ttctcattaa aacctacccc tttaaaaatc atacaaatta gttctactgt aaaaaaattc
      481 a
//
LOCUS       DAZFTN010000480          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFTN010000480 DAZFTN010000000
VERSION     DAZFTN010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..335)
                     /locus_tag="ACHKV2_005673"
     CDS             complement(<1..335)
                     /locus_tag="ACHKV2_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6943306.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELV"
     gene            complement(325..>476)
                     /locus_tag="ACHKV2_005674"
     CDS             complement(325..>476)
                     /locus_tag="ACHKV2_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6943307.1"
                     /translation="RIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSG
                     HNNVK"
BASE COUNT          162 a          105 c           66 g          143 t
ORIGIN      
        1 accaactccg gttcagaata ttttatcaaa tatgatttat aaccactcat gcctgtctca
       61 acaaaattaa caaccgacgc tacattattg aatgaaagat tctcattctg actgttataa
      121 tatatttcct tcccaaccgg catcattaca aacattgaca atagcaaagc aaccccatta
      181 agtgtcatat ttgatggcac ttgctgaagt cccaatgcgt ttcggacaat aacaaaaaca
      241 atcgagaatt taataaaaca tgtcccggat gctattataa acggcaatag tgtaaataat
      301 gatagtatcg ctatcagcga aatgctattt gacattatta tgccctgata accaagaatg
      361 aatctctacg cccaatttat catccacttc aacaagttcg ccatagccag ttagcgcgcc
      421 atttacacgt atctctatat ttttctcaga ttcaggaagc agagatatta ttcttt
//
LOCUS       DAZFTN010000481          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFTN010000481 DAZFTN010000000
VERSION     DAZFTN010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..462
                     /locus_tag="ACHKV2_005675"
     CDS             <1..462
                     /locus_tag="ACHKV2_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HFO6943308.1"
                     /translation="EERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYL
                     DVETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEW
                     VELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
BASE COUNT          105 a          111 c          136 g          120 t
ORIGIN      
        1 gaagagcgta acagtctgct gtggggaggg attgccacgg tcagtgtggc tgccggtgat
       61 gtggttcaga tcgaacgtca ggtgaccatg tatcgccaga acgtgtacgg cgaaagtgat
      121 cccagctatc tggatgtgga aaccatctac accctctcct ggctgcgtta ttccctgcgt
      181 acttttatca cacaacgttt tccgcgccat aaactggctg atgacgggac gcccgttcgt
      241 gccggtcaga atatcgtgac gccggagatc atgaagcttc agttaatttc gctgggtgaa
      301 gagtgggtgg aactcgggct ggtcgaaaat ctggacacct ttaaaaagaa tctgcttgtg
      361 gagcgtaatg cgtcagatcg aaaccgtctt gacgtgttat gcacgccgga tctggttaac
      421 cagttccgct tcctggcagc acagatccgt ttcattttgt gaggtaaggc at
//
LOCUS       DAZFTN010000482          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFTN010000482 DAZFTN010000000
VERSION     DAZFTN010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            186..>472
                     /locus_tag="ACHKV2_005676"
     CDS             186..>472
                     /locus_tag="ACHKV2_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO6943309.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRD"
BASE COUNT          147 a           81 c           98 g          146 t
ORIGIN      
        1 tcatttcagc ttattgatag aaagattgat tgccagtctg tttagagccg tacagcagca
       61 aactttcata accaggttga tcctgaattt gcagtacgaa aagttaaaaa tgagcgattt
      121 ctgtaagact ccccattttg ccaggcttat gattcgcaga tcattcaagt gcaaaggagc
      181 gatgtatgga ttgttcaaaa tgcaatggtt atgcgacgat gttattaaat atggtgcagg
      241 gttctgaccc ggttaattta ctggaattac atggtttcct tgaacatttc gcatattatg
      301 tttcatttgg caaatttaat gctggccacc agcgctataa tgcatttaaa aaattcgtca
      361 gtgagatttc ggagatcagc gcaaatgata tcaatatgac gattaaaacc gggcaatcca
      421 gacatgaaaa tgtgatctct ataaatatga atgatgcaat tcctcgtgat ga
//
LOCUS       DAZFTN010000483          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFTN010000483 DAZFTN010000000
VERSION     DAZFTN010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..330
                     /locus_tag="ACHKV2_005677"
     CDS             112..330
                     /locus_tag="ACHKV2_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943310.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          150 a           58 c           95 g          166 t
ORIGIN      
        1 gccaaatatt attgcaaaat attctctaaa ttatgaatta aatatccata agcattattg
       61 cctgggaact ggacatataa ttactcttct gtgctaaagg aacgttctga aatgtttaag
      121 tatgcagaga tgttgatggc ggaagaagat gataaagtat gtgaacttat ttttaaacaa
      181 caagataatt tgattttaaa aataaatgtt agatttccca gaataattat aaagaaatac
      241 cgaacattac aaggtaaagt cgtatggcta tggatattgc catttgttat tgaatggtta
      301 attgtgtgta ctttgggtgt aaacagttag cttggtaaaa gtaaatatcc tgattgttgt
      361 ttgttaagga gtgagatgcc tgtgcgtctg gtgtctatta tattcccaat attattggcg
      421 ctattcttgc tggcgtaggc atcgccatac tatgaatatt gtgtttgtc
//
LOCUS       DAZFTN010000484          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFTN010000484 DAZFTN010000000
VERSION     DAZFTN010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>468
                     /locus_tag="ACHKV2_005678"
     CDS             <1..>468
                     /locus_tag="ACHKV2_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943311.1"
                     /translation="TYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCL
                     SGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKE
                     KIISDLDKVQVACYYLTILLAFERRTSLTSTLATLYKMLISEREVSEYECQLL"
BASE COUNT          169 a           84 c           77 g          138 t
ORIGIN      
        1 acacatatcg cccggataat gagatggcat tttactatca tattcattct tttctcactc
       61 agataccgga taatgaggat gatatatatc atgctgcaag gacatatctg cgaaattacg
      121 ttaagttatg tttatccgga tacacgccag cgaatgcgca tttcaaagat atctttgatg
      181 gcgtatatga attcattcgt aatattcgca aaaacagtac accaggaaaa acaaaactta
      241 tcgcaactat caacacatgc aaagaaacct gtaaacatct gctttattta agtaatgaag
      301 acaaggaaaa aataatttct gacttagata aagttcaggt tgcatgttat tatctcacta
      361 tattactggc tttcgaaaga cgaacttcat taacaagcac cctggcaact ttatataaaa
      421 tgctgataag cgaaagagaa gtttcagaat atgaatgcca gttattat
//
LOCUS       DAZFTN010000485          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFTN010000485 DAZFTN010000000
VERSION     DAZFTN010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..82
                     /locus_tag="ACHKV2_005679"
     tRNA            6..82
                     /locus_tag="ACHKV2_005679"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Arg,seq:tcg)
     gene            96..172
                     /locus_tag="ACHKV2_005680"
     tRNA            96..172
                     /locus_tag="ACHKV2_005680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Arg,seq:tct)
     gene            complement(301..444)
                     /locus_tag="ACHKV2_005681"
                     /pseudo
     CDS             complement(301..444)
                     /locus_tag="ACHKV2_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          117 a          120 c          118 g          106 t
ORIGIN      
        1 acataccgct attagctcat cgggacagag cgccagcctt cgaagctggc tgcgcggggt
       61 tcaagtcccc gatggcggtc cattatcggt attcagcgtt gttagctcag ccggacagag
      121 caattgcctt ctaagcaatc ggtcactggt ccgaatccag tacaacgcgc cacaccacac
      181 ttatctgccc tgactctctt ttgcgggctt tttattacag gaaagacacc ggacagtgaa
      241 atgttaaatg cctcacaatt caggcagttg aatgttgcct gacatgctga gcgtttgtta
      301 aaaaaatttc gcatggtgaa tccccctgtg cggaggggca atcagcaacc aggtatatgt
      361 gataatcgcg gattcaggtg ctgatactga attcaccggg aggcacccgg caccatgcaa
      421 gaaaaagaat gtgcatgcaa acatgcccct ctccggaggg g
//
LOCUS       DAZFTN010000486          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFTN010000486 DAZFTN010000000
VERSION     DAZFTN010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..382
                     /gene="espX1"
                     /locus_tag="ACHKV2_005682"
     CDS             <1..382
                     /gene="espX1"
                     /locus_tag="ACHKV2_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO6943312.1"
                     /translation="GEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKH
                     LGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTS
                     PEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          187 a           82 c           71 g          119 t
ORIGIN      
        1 tggagaaata aacaacctct tcattaaaaa aagcaacata tttggaacat cgttcactaa
       61 taccatgata aaaaatttgc gctgtgaaga tattatgcct ggccgatgga ccactcaact
      121 tgtcaataag cacttaggtt acagatacac aggagtattc aaaaccctgg caagtattga
      181 tgacaaaccg tcccgtttcg aaattttgat accattggta caaactttag ttcgcgacaa
      241 tgtaaagcta aataatgatg tgtataaaga actgaaaaag tttatgcacg attacgacaa
      301 aacatcccca gaaatgagaa aataccttca gagcatcaac gagtctatgt tccttatgaa
      361 aaaaatatct catcaagatt aaaacacccc aaaaaagaag taatttcaga atgaaattac
      421 ctgtgataat tattaattaa ctgcagagaa ataaaaaat
//
LOCUS       DAZFTN010000487          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFTN010000487 DAZFTN010000000
VERSION     DAZFTN010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.493"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>449
                     /locus_tag="ACHKV2_005683"
     CDS             <1..>449
                     /locus_tag="ACHKV2_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943313.1"
                     /translation="DTDHITIGIDNQEPIKLPASYKDKELVRTIINDNIVEKTHDDIN
                     NAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIKSPANDENKKVGQTIINENIVENTH
                     DINNKKMIFSAIKEIYDEDPSFIFDKISHKLRHTVTEFDESGKSEPTG"
BASE COUNT          187 a           77 c           80 g          105 t
ORIGIN      
        1 gatacagacc atattactat tggtatcgac aaccaagaac caataaaatt acctgcgagt
       61 tataaagaca aagaactcgt ccgtactatc attaatgaca acatagttga gaagactcat
      121 gatgatataa ataatgctga atttactgat ggtggtattg atgatcgtag tgatattggc
      181 attgactcca atgatacaga cgatattgat attgataatc aagaaccaat aaaatcaccc
      241 gcgaacgatg aaaacaaaaa agtcggccaa accatcatca atgaaaacat agtagagaat
      301 actcatgata tcaataacaa gaaaatgatc ttcagcgcaa taaaagaaat atatgatgaa
      361 gatccaagtt ttatcttcga taaaatatca cacaaactca gacatacggt aacggaattc
      421 gatgagagcg ggaaaagcga accaacggg
//
LOCUS       DAZFTN010000488          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFTN010000488 DAZFTN010000000
VERSION     DAZFTN010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..440
                     /locus_tag="ACHKV2_005684"
     CDS             <1..440
                     /locus_tag="ACHKV2_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943314.1"
                     /translation="KRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMI
                     LSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETI
                     SERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          128 a           86 c           82 g          147 t
ORIGIN      
        1 gaaaacgtat tcctgaactt tcagcacttc caccttcatt aagaaagaaa aattttactg
       61 acgcatataa tgcagcatct tctcatatcg aatactggat aggtgctggc gtctcattta
      121 tctcgatgat gattttatcc agagtatatg actttctttt acccgcccaa gatactttcc
      181 cgggtgatat catcagaagt ttatgtgttg tgtgtccatc catattaatc tggtttcagt
      241 tttcggtata tgtcatgagg aaatattaca gacatattct ggtgcgagga aaagaaaccg
      301 aaaccatttc agaaaggttg atacgtgagg ctgatacacg agaatatgaa ctatggcgtc
      361 cggtccgtcg ctttttctcc attgttttcc tcattgttct attaggttgc attcattcac
      421 tgataacaac tataaaataa aag
//
LOCUS       DAZFTN010000489          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFTN010000489 DAZFTN010000000
VERSION     DAZFTN010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(47..151)
                     /locus_tag="ACHKV2_005685"
     CDS             complement(47..151)
                     /locus_tag="ACHKV2_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943315.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(267..>440)
                     /locus_tag="ACHKV2_005686"
     CDS             complement(267..>440)
                     /locus_tag="ACHKV2_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6943316.1"
                     /translation="DGQHVIILCDGAFVLYAQYRDGEFFDVVRNGDEFFETQSRNVTD
                     WMPLPEPPQEVRQ"
BASE COUNT          136 a          123 c           77 g          104 t
ORIGIN      
        1 gcaaaccgtt tttacccgat atgggaattc ccatatcgta atgaattcag ttccctagtc
       61 gatccatcaa aaacacaacc aggcagtaaa cgcccacaac agcaataaca gccagcgcac
      121 cttccattgc cagtgaaata tcatccgaca tattccctcc tttggtgtga atcccggcga
      181 acgtttttac ccccaccgac aaataacata tactaaaaaa tcaatagcta tagcaacgcc
      241 tgtaattgca aaggcttcag gccagatcat tggcgcacct cctgcggcgg ttctggtagc
      301 ggcatccagt cggttacatt gcggctctgt gtttcgaaaa attcatcacc attacggact
      361 acatcaaaaa actcaccgtc tcgatattgc gcataaagaa cgaatgcgcc atcacataaa
      421 ataattacgt gctgaccatc
//
LOCUS       DAZFTN010000490          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFTN010000490 DAZFTN010000000
VERSION     DAZFTN010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.496"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            327..431
                     /locus_tag="ACHKV2_005687"
                     /pseudo
     CDS             327..431
                     /locus_tag="ACHKV2_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          114 a          112 c           79 g          126 t
ORIGIN      
        1 taagcaataa aaaacagctt caggtaatga ggagtatctc aatttttaaa cataaaatgc
       61 gaattattta gtacaaaaag tgaacttttg atgatctttc cctaatctca cataggatca
      121 gatctcgtct gatcgttccc cctgcgctca ctaccatggc tctttaccac agcagccggg
      181 gttgtttggg accgactttc gacagcagat cccggtgggc ctgccaccat ctcttcacaa
      241 gcttatgctt attacatgca aaacgtcccg ttttgcatct ttgtgcctac ggcacaccga
      301 tgatctttcc ctgtgatctt tccctgaatc tgcccctgag aacctgaatc aaacgtctga
      361 ctcgcagaac catctgtttc tcgcgctctg tagaacttca tgaaaaagtc atcggtgctt
      421 ttatcgaaaa a
//
LOCUS       DAZFTN010000491          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFTN010000491 DAZFTN010000000
VERSION     DAZFTN010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.497"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>419)
                     /locus_tag="ACHKV2_005688"
     CDS             complement(<1..>419)
                     /locus_tag="ACHKV2_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO6943317.1"
                     /translation="LNGPVFENCFFEECNFESVSLVGFDKVTCESYDVLCNVKPNNKI
                     PIYGMFKGCFLYQCEMKNFKIETSKIYSINQDPQRGDKKVGAYLFMQSFVYASNLQDG
                     VCKEASVIASSLLSCNIAKLNGVGMDFIETSFYGKTRG"
BASE COUNT          137 a           89 c           53 g          140 t
ORIGIN      
        1 ccgcgagttt ttccataaaa cgaagtttct atgaaatcca ttccaacacc attaagcttt
       61 gctatattgc aagataataa actgcttgca ataacacttg cttctttaca cactccatct
      121 tgaagattac ttgcatatac aaatgactgc ataaacagat atgcaccgac tttcttatcc
      181 cctctttggg gatcttgatt tatagagtat atttttgatg tctctatctt gaaatttttc
      241 atttcgcatt gatacaaaaa acacccttta aacataccgt aaatcggtat tttattattt
      301 ggttttacgt tacaaagaac gtcatagctt tcacaggtca ctttatcaaa gccaacaagg
      361 gacacactct cgaaattaca ttcctcaaaa aaacaatttt caaatactgg gccattaag
//
LOCUS       DAZFTN010000492          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFTN010000492 DAZFTN010000000
VERSION     DAZFTN010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..389)
                     /locus_tag="ACHKV2_005689"
     CDS             complement(<1..389)
                     /locus_tag="ACHKV2_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943318.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPTEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGILSVES"
BASE COUNT          122 a           62 c           81 g          154 t
ORIGIN      
        1 ttgctttcga cactcaaaat accttcgttg ttttcacctc gggtaaaggt aagggtatag
       61 ggttcataat ccgctgcaaa ttcattttta tacgttatga catgttcgac aaaatgtctt
      121 attggtacga aaccataact gtgaacgtca attttttggg tcttgatact atcaattgca
      181 tctgaggctt tcgtgaaaaa agccaataaa ttagctatgc tgtctgttga ggttgataac
      241 ccgtatctac tttgagatat ttttagaaac tcatcgcaaa aaaactcaag tttggcggtt
      301 tgttgtgatt ctttgtaaat ttctgttggt tgcaatttat tattttgtaa aatattacag
      361 cgagataagt gaatagatgt atttggcata aatgattcct tgtattgtga gttaacaag
//
LOCUS       DAZFTN010000493          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFTN010000493 DAZFTN010000000
VERSION     DAZFTN010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>413
                     /locus_tag="ACHKV2_005690"
     CDS             <1..>413
                     /locus_tag="ACHKV2_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFO6943319.1"
                     /translation="EQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGF
                     LDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYE
                     VYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEI"
BASE COUNT          126 a           61 c          109 g          117 t
ORIGIN      
        1 gggagcagac ttaccggtat gtgccgaatc cttgtgggtg ggttgatccg ctgggattgg
       61 ctgcaagttc taaaatcagc agtttgatgg actatattgg cgatggtcgt cgtgttagtg
      121 ggcatacggg tttcctggat ggggttcgtt tatcacgtag tcaaataaac aatattgcta
      181 aagaaatgga gaagctagga attaaagtaa taaggaaagc agataaatat ttgccaccaa
      241 atgctagggc agcttttgat tatggtcttc gcaatattta tcttaggaaa aatgctacct
      301 tatatgaggt gtatcatgaa gtgattcatg ctaagcaatt tgcgaaaatt ggacgagaag
      361 catacgaagc actaggacgt ttatctaggg aggaacacgt tctaaatgaa ata
//
LOCUS       DAZFTN010000494          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFTN010000494 DAZFTN010000000
VERSION     DAZFTN010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>412
                     /locus_tag="ACHKV2_005691"
     CDS             <1..>412
                     /locus_tag="ACHKV2_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO6943320.1"
                     /translation="GETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          123 c          133 g           59 t
ORIGIN      
        1 gcggggagac agtacgctac cgctacgatg atgcgtacag tgagttaccg gcgacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacggtcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgct atgaatacga ccgcttcggc cagatgacgg
      181 cggtccaccg cgaggaaggc atcagccttt accgccgcta tgacaaccgt ggccggttaa
      241 tctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggaatacgac ct
//
LOCUS       DAZFTN010000495          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFTN010000495 DAZFTN010000000
VERSION     DAZFTN010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>412
                     /locus_tag="ACHKV2_005692"
     CDS             <1..>412
                     /locus_tag="ACHKV2_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO6943321.1"
                     /translation="GDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          117 c          131 g           67 t
ORIGIN      
        1 gtggggacgt catccggtat gcttatgata atccgcacag tgaattaccg gccacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacgggcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgtt atgaatacga ccgctttggt cagatgacgg
      181 cggtccaccg tgaggaaggt atcagccgtt accgccgcta tgacaaccgt ggccggttaa
      241 cctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcactccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aagcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtatgac ct
//
LOCUS       DAZFTN010000496          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFTN010000496 DAZFTN010000000
VERSION     DAZFTN010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>402)
                     /locus_tag="ACHKV2_005693"
     CDS             complement(<1..>402)
                     /locus_tag="ACHKV2_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943322.1"
                     /translation="ADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPAAP
                     SRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLGSALYWIAA
                     SINIKPGHDYYFYIRSVNTVGKSAFVEAVGRA"
BASE COUNT           89 a          117 c          112 g           84 t
ORIGIN      
        1 cgcccgaccg acagcctcca cgaatgccga tttgccaaca gtattcacac tgcggatata
       61 aaaataataa tcatggcccg gtttgatatt gatactggca gctatccagt acagcgccga
      121 gccaagatag cgggctgcgg tttcaacctg cctgatatcc gcaatccgct tttccgagaa
      181 ccagaactca aactgtaccg tcgggtcata aacggcaaga tgcggcgtgg cggttatctg
      241 aaaatagccc ggcgtcagct caatccgcga cggtgctgcc ggtgcggcaa tccggaaggt
      301 ggtggtggca ggttcaccct gctggccata gctgtttatc gcccgcaccg tcagggtgta
      361 ttccccgagc ggcaggccgc tgaaacggtg ctccgtgtct gc
//
LOCUS       DAZFTN010000497          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFTN010000497 DAZFTN010000000
VERSION     DAZFTN010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..397
                     /gene="tccP2"
                     /locus_tag="ACHKV2_005694"
                     /pseudo
     CDS             3..397
                     /gene="tccP2"
                     /locus_tag="ACHKV2_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT          119 a          114 c           66 g           98 t
ORIGIN      
        1 tgataaatag cattaattct ttttttccgg ccttcctcgc agtatatcaa gtgctatacg
       61 cagcagtacc ttcactgtct cgcagcacaa aagcacacct aacacggtaa aaaccagctc
      121 acctttttct cccagcaaca gtcctgccag tgcaacaact atttttaagg taaaaaattc
      181 ttatacagaa tcaggacttc aacgtcccac atcttatact cagtcaagta tagaaaaaaa
      241 cgctttacat cggcctctac ctgatgtagc tcagcgtctg gtgcagcatc ttgcagagca
      301 tggcattcaa ccagcccgga gtatggctga acatattcct ccggcaccta actggcctgc
      361 gccaccaccg ccagtacaaa atgaacaatc aagacct
//
LOCUS       DAZFTN010000498          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFTN010000498 DAZFTN010000000
VERSION     DAZFTN010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..165
                     /locus_tag="ACHKV2_005695"
     CDS             <1..165
                     /locus_tag="ACHKV2_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943323.1"
                     /translation="EEQAEWLIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRP
                     SRTFQQRIWR"
     gene            167..>396
                     /locus_tag="ACHKV2_005696"
     CDS             167..>396
                     /locus_tag="ACHKV2_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943324.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAG"
BASE COUNT           89 a          111 c          110 g           86 t
ORIGIN      
        1 gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga ggttaaaaaa
       61 gcccttagcc tcgactaccg tcactggata atctccgtca ggctccctta ctccgaacgg
      121 ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaatgtgc gggtattact
      181 tcgacctgtt ctggtaccgg aactcggtct ggttatcgtt aagccaggcc gtgaatcaat
      241 gtcagcattc cataacggca gaatactggt ggagccggaa ccaaaaagca tgcgagctct
      301 gccgtccggg gttgtacctg ccgttcacca gccgctggcg gaagataaat cactactgcc
      361 atttttcagc gatgagcggg tgatccgtgc tgcggg
//
LOCUS       DAZFTN010000499          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFTN010000499 DAZFTN010000000
VERSION     DAZFTN010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          135 a           88 c           55 g          117 t
ORIGIN      
        1 acccccggta gagttgcccc tactccggtt ttcgagacct atgctatggg ttaataaaat
       61 caatatatta tgtgttttat ttggaataaa tattctatat ttaggattga aaaatcagat
      121 ggttagcatc aaacaacctc agaatattcc aagcaaacag gttaaaaata aaactgcacc
      181 cgaacaattg ataacgacag aaaacgcctt ttccgagcca ccaaaattac ttcgcatcct
      241 atttattgct cacgttaacg ccttgtataa ctcgagctct ccacggtatt taacctctct
      301 tctgtttaac tataattcca ataaatctcg tcactgagct caatcacttc gaaagtcaag
      361 cacaatatga atttcacata agctatcaat gaaaa
//
LOCUS       DAZFTN010000500          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFTN010000500 DAZFTN010000000
VERSION     DAZFTN010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>390
                     /gene="wzx"
                     /locus_tag="ACHKV2_005697"
     CDS             <1..>390
                     /gene="wzx"
                     /locus_tag="ACHKV2_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO6943325.1"
                     /translation="ISLSVISSLISKILSLLSLILTVSLTLPYLGQERFGVWMTITSL
                     GAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLAGLSFVITAICYITSG
                     MIDWQLVIKGINENVYAELQHSIKVFV"
BASE COUNT          116 a           58 c           77 g          139 t
ORIGIN      
        1 ttatatcctt gtctgttatt tcaagtttga ttagtaaaat actctcacta ctttctctta
       61 tattaactgt aagtttaact ttaccttatt taggacaaga gagatttggt gtatggatga
      121 ctattaccag tcttggtgct gctctgacat ttttggactt aggtatagga aatgcattaa
      181 caaacaggat cgcacattca tttgcgtgtg gcaaaaattt aaagatgagt cggcaaatta
      241 gtggtgggct cactttgctg gctggattat cgtttgtcat aactgcaata tgctatatta
      301 cttctggcat gattgattgg caactagtaa taaaaggtat aaacgagaat gtgtatgcag
      361 agttacaaca ctcaattaaa gtctttgtaa
//
LOCUS       DAZFTN010000501          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFTN010000501 DAZFTN010000000
VERSION     DAZFTN010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(16..339)
                     /locus_tag="ACHKV2_005698"
     CDS             complement(16..339)
                     /locus_tag="ACHKV2_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943326.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGESGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEISKFDNIYCDQLECLFNEQTG
                     LATRL"
BASE COUNT          122 a           81 c           68 g          118 t
ORIGIN      
        1 cccccatatt aaaaattaca atctggttgc aagtccggtt tgttcattaa ataaacattc
       61 aagctgatca caataaatat tatcaaattt tgatatttct tttactgtag ctccacggac
      121 acgggaaaat gtcaccaggt atgtatcaga gaaggttaat ttgattttaa ccaaattaat
      181 tcccttcatt gcaaaatttg aaggtaaacg aaagcaaaga ccagactcac cattttcatc
      241 aaaacaggca aagttctttg cgcctgtcat agcaataaag cggtttccac caagctgatt
      301 aagaatttca gtagcaacaa atttggcgtg gttttccatt tttaagctcc tgccgctgag
      361 cggctttggt gattggggac catcccctc
//
LOCUS       DAZFTN010000502          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFTN010000502 DAZFTN010000000
VERSION     DAZFTN010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..264
                     /locus_tag="ACHKV2_005699"
     CDS             <1..264
                     /locus_tag="ACHKV2_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6943327.1"
                     /translation="AVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLGIHLKQAGSDFK
                     LHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            261..>384
                     /locus_tag="ACHKV2_005700"
     CDS             261..>384
                     /locus_tag="ACHKV2_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFO6943328.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVI"
BASE COUNT          100 a           91 c           99 g           94 t
ORIGIN      
        1 gctgtccgtg accctcaaat ggaaatgacg ttgacctgga gtggtacgca gagtgacttt
       61 gacgattcgg ccgtagccac ggatctcaat ggattaggaa tacacctcaa acaagccggg
      121 agcgatttta agctgcatac gcccttagtg gtgaatgaaa catcccttcc ggtactaacg
      181 gcagtacccg taaaaaagag cggtgtcgac ttacccgagt cagactttga agcatgggct
      241 acgttacagg tggattacca atgaagaata accgtgcgtg ggcgcttatc agtggtctga
      301 tattgttcag cggaacggcc ccagctgccg ataacctgca ttttaccggt aatttgcttg
      361 gtaaatcctg tactcctgta atca
//
LOCUS       DAZFTN010000503          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFTN010000503 DAZFTN010000000
VERSION     DAZFTN010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>383)
                     /locus_tag="ACHKV2_005701"
     CDS             complement(<1..>383)
                     /locus_tag="ACHKV2_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943329.1"
                     /translation="GIKIQETVSGNDRLFFYPTPLKNGCFILHNPLYSNSFPSLYSHE
                     LFDLIEAYRNPTYSYLQNNIINRYIHTVSEYAGKDGIANMSLSIYAYSTCRFWSKESV
                     AVGDTIKLSNYEHIEFSFNSFSKDY"
BASE COUNT          135 a           64 c           57 g          127 t
ORIGIN      
        1 gtaatcctta ctaaatgaat taaagctaaa ctcaatatgc tcataattag acaattttat
       61 tgtatctcct acagccacac tttcttttga ccagaaacga catgtcgagt atgcgtatat
      121 agataatgac atattggcaa taccgtcttt tccagcatac tcagaaacag tatgtatata
      181 tctattgata atattgttct gtaaatacga atacgttgga tttctatatg cttcaatcaa
      241 atcaaataat tcatgtgaat ataaagaagg gaatgagtta gagtaaagtg gattatgcaa
      301 gataaaacat ccatttttta atggagtagg gtaaaaaaac aacctgtcat ttccactgac
      361 tgtttcctgt atctttatcc cca
//
LOCUS       DAZFTN010000504          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFTN010000504 DAZFTN010000000
VERSION     DAZFTN010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>382)
                     /locus_tag="ACHKV2_005702"
     CDS             complement(<1..>382)
                     /locus_tag="ACHKV2_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO6943330.1"
                     /translation="DAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADWT
                     ATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVSS
                     IGKAVTAKEVITRTVKVTNVGKPSV"
BASE COUNT           70 a          130 c           84 g           98 t
ORIGIN      
        1 ccacggaagg tttgcccacg ttagtgactt tcaccgtgcg ggtgatcact tctttcgccg
       61 tcacggcctt accgatactg ctgacccagc cacggaacac atccaccgtg ccatttggga
      121 aacggatttt ataggcccgc acatccccgc tttcaaacca gcctataagc cctttctgac
      181 cttcttctcc cggtttccag gccagcgtaa aactggtatc tcctgcagac ttctgcccct
      241 gcccggtcgc ggtccagtcc gcgtcttcat catccaggta gttatcatcg tagggttctg
      301 ccgtcatctc gcccggcgtc agatccttca ccttagccag tcgctgccag tcatcgtctg
      361 acaacgggtt tgcataagca tc
//
LOCUS       DAZFTN010000505          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFTN010000505 DAZFTN010000000
VERSION     DAZFTN010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..316)
                     /gene="escQ"
                     /locus_tag="ACHKV2_005703"
     CDS             complement(<1..316)
                     /gene="escQ"
                     /locus_tag="ACHKV2_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO6943331.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGDVSVDD
                     VQF"
     gene            complement(306..>381)
                     /locus_tag="ACHKV2_005704"
     CDS             complement(306..>381)
                     /locus_tag="ACHKV2_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO6943332.1"
                     /translation="DYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          119 a           90 c           69 g          103 t
ORIGIN      
        1 taaattgaac atcatcaact gacacatcac cttccggtaa ggcagtctgc attacccata
       61 agatcatgag ttttatcgta tcttccggta tcagccggct ttcgctatac gggaataact
      121 cttggcacca ctgaatgaac tgttcttctg atatccagaa acgcccctca tagcccgagt
      181 atgtcaacgt gaaacgaata ccatgaccgc tgacctcacg aaaatatgca tggcaagcat
      241 gacctttgga cgtaatatag aggcgaccgg ttccgataac tggcaacaac ggcaaataaa
      301 aatcgttaat tttcatattc aattgtgaac tcaatggctt catcaggaaa gatttctttt
      361 aacttatcga aaacatagtc t
//
LOCUS       DAZFTN010000506          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFTN010000506 DAZFTN010000000
VERSION     DAZFTN010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..143
                     /locus_tag="ACHKV2_005705"
     CDS             <1..143
                     /locus_tag="ACHKV2_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6943333.1"
                     /translation="NLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(225..>381)
                     /locus_tag="ACHKV2_005706"
     CDS             complement(225..>381)
                     /locus_tag="ACHKV2_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFO6943334.1"
                     /translation="QINIALDQKGSGNFSAWVIEACRRRLINEKHAQFVPNKDKHDQS
                     TCSDRFT"
BASE COUNT          118 a           66 c           87 g          110 t
ORIGIN      
        1 tgaacctgac agaaataaaa caagcggtca acagactaca ttctgagttg ggaatgggat
       61 ttgaagagcg gcgggtattc cgacgatata aagggacagg agaacagaat acgtccggaa
      121 acgcgcggtc aaaaaaatgc taaaaaatat ctgagagcgt tattgcctgt taccataaga
      181 aaaagcgact ttagtggtcg ctttttgtgt catatataag tcgtttaagt aaacctgtct
      241 gaacaggtgc tctggtcgtg tttgtctttg ttgggtacaa attgagcatg tttttcatta
      301 attaatcttc ttctgcaggc ttcaataacc cacgctgaaa aattacctga acctttctga
      361 tcaagagcga tgttaatttg t
//
LOCUS       DAZFTN010000507          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFTN010000507 DAZFTN010000000
VERSION     DAZFTN010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..314)
                     /locus_tag="ACHKV2_005707"
     CDS             complement(<1..314)
                     /locus_tag="ACHKV2_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943335.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGV"
     gene            complement(314..>377)
                     /locus_tag="ACHKV2_005708"
     CDS             complement(314..>377)
                     /locus_tag="ACHKV2_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943336.1"
                     /translation="ITLPDGSRHPMIFYADMKGE"
BASE COUNT           72 a          103 c          107 g           95 t
ORIGIN      
        1 acgccttccg tgatccctgc atccgtggtc agcatcagtt gtaccggtcc gtcagcgcgg
       61 gaagccggtt tacggatgac accccagaat gcgcagtgtt tcaggagttc ggcttcatca
      121 gcttctgtcg gaattatctg ccgggagatc caggccagat gttcatgctc ctgtgctgac
      181 agacccgcca gtgcatacgc aatggcattc agggtggttt cattcacgcc cggctgcgaa
      241 ccgggcaggc gctggctgat atcctgttgt gtctgtgtga tcagttgcgc cagtggtaag
      301 ggctgataag gcatcattcc cccttcatat cagcataaaa aatcatcgga tgtctgcttc
      361 cgtccggcag ggtgatt
//
LOCUS       DAZFTN010000508          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFTN010000508 DAZFTN010000000
VERSION     DAZFTN010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.denovo.515"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>377)
                     /locus_tag="ACHKV2_005709"
     CDS             complement(<1..>377)
                     /locus_tag="ACHKV2_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6943337.1"
                     /translation="GADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAW
                     CKPEWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTIS
                     VFKLTNKPVAFEIRIVPKTPENQA"
BASE COUNT           81 a          129 c           83 g           84 t
ORIGIN      
        1 gcctgatttt ccggcgtttt gggtacaatc ctgatctcaa acgccaccgg cttattggtc
       61 agcttaaaca cgctgattgt cggcccaagt ggctgaccaa cgggctggcc cgtcgccgga
      121 tcatcatgac tgcggatata atccgccacc cgcttcacat caccttctcc ggggaaaatg
      181 tcagggttat tatcctgaac aaaagtcaca ccaacactac ccgcctcagg ccattcaggc
      241 ttacaccatg cccgcgtcac gcccggtact tcccgtgccc agcgttcaaa atcatactgt
      301 gttccccctg atggcgggtt ctgtacccgg aataccagcc tggacagcaa ctccggcacc
      361 gtttccacat ccgcacc
//
LOCUS       DAZFTN010000509        17380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.guided.094, whole genome shotgun sequence.
ACCESSION   DAZFTN010000509 DAZFTN010000000
VERSION     DAZFTN010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.guided.094"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKV2_005710"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKV2_005710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6943338.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKV2_005711"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKV2_005711"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6943339.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHKV2_005712"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHKV2_005712"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6943340.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKV2_005713"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKV2_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6943341.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHKV2_005714"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHKV2_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6943342.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHKV2_005715"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHKV2_005715"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6943343.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHKV2_005716"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHKV2_005716"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6943344.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHKV2_005717"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHKV2_005717"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6943345.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHKV2_005718"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHKV2_005718"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6943346.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHKV2_005719"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHKV2_005719"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6943347.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /locus_tag="ACHKV2_005720"
     CDS             12901..14034
                     /locus_tag="ACHKV2_005720"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6943348.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14122..14655
                     /gene="blc"
                     /locus_tag="ACHKV2_005721"
     CDS             14122..14655
                     /gene="blc"
                     /locus_tag="ACHKV2_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6943349.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="ACHKV2_005722"
     CDS             complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="ACHKV2_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6943350.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="ACHKV2_005723"
     CDS             complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="ACHKV2_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6943351.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="ACHKV2_005724"
     CDS             complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="ACHKV2_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6943352.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15579..16145)
                     /gene="efp"
                     /locus_tag="ACHKV2_005725"
     CDS             complement(15579..16145)
                     /gene="efp"
                     /locus_tag="ACHKV2_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6943353.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16187..17215
                     /gene="epmB"
                     /locus_tag="ACHKV2_005726"
     CDS             16187..17215
                     /gene="epmB"
                     /locus_tag="ACHKV2_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6943354.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4312 a         4566 c         4484 g         4018 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaattt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 agcaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgtttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctgaccg atgctgttac
      481 cgcaaatcgg cgatttaccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagcca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgaacagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gcctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatttagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcgc
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccaga agtcgtagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggga cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 ctgaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattgaaca
     3721 gccaacgcca gcttgaggca gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gatacgctac tgctggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaagatgcg ctgaaagaag tgaacgaagc gacccaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtcacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgctac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc tcgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccaacgacgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcacatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcag tccttgcggc tagtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaatc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtaccggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtga caattcgcga tcttactggt agcgtgacga
     5881 aaattaatac ccgcgcaacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gctgaataat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag aacgacaacc accgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatagcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtgctcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tgatcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggt tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaaac tgcggcacag ctggaatacc ggcccgcaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcatccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggttgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggtgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggctccaa ctccctagcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ttccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcacca agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctacc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcaacaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 gctggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggg
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacactgct ggcggcctgc cattgcaggt gccggatgaa
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggcaagg cagttcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcgtt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca
    13861 actcctgcag tacgagcatc atgggtgcat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
    14161 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgaccgt agtgaataat
    14221 ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgcttt
    14281 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
    14341 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
    14401 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
    14461 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggaccg
    14521 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
    14581 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
    14641 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
    14701 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
    14761 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
    14821 atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc
    14881 gtcaaacgac taaagccgtg ggtatatttc aggcctacgg cccataccac ttccagcaga
    14941 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
    15001 gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
    15061 ggtggcgggg gaatttcaac tattaccttt gtgactatcg cagaaaaaac aaacggcacg
    15121 acacagctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc
    15181 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
    15241 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
    15301 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
    15361 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgggag
    15421 atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg
    15481 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
    15541 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
    15601 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
    15661 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
    15721 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
    15781 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcgttg
    15841 tcgccaattg cttttgcgtc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
    15901 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
    15961 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
    16021 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
    16081 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
    16141 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
    16201 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
    16261 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
    16321 caacgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
    16381 aggcaatccg gacgatccgc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
    16441 cgcgcccgga ttctccaccg acccgctgga agaacagcac agcgtagtgc ctggtttgtt
    16501 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
    16561 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
    16621 aactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
    16681 cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat
    16741 cccgcatatc aaacgtctgc ggattcacag tcgtctgccg attgtgatcc cggcacgcat
    16801 cacagatgcg ctggttgaac gcttttcaca ttctacgctg caaatcttgc tggtgaatca
    16861 catcaaccat gccaatgaga tagatgaaac attccgtcag gcgatggcta agttgcgccg
    16921 ggcgggcgtt actttgctga accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
    16981 aacgctggca aacctgagta atgcgttgtt cgatgcaggc gtgatgccgt attacctgca
    17041 tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
    17101 gattatgcgt gagttgctga cactggtgtc gggatatctg gtgccgaaac tggcgcgaga
    17161 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
    17221 taaacaggga agtttaatct ttgcccgtta cataaccttt aattattcat atacattgct
    17281 gttgttactc tttatataac caacaccaat aattaacctt tcttttctgt ttatatagag
    17341 tgagtacggc tatttatgtt attttaacaa ctattactac
//
LOCUS       DAZFTN010000510        12626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.guided.128, whole genome shotgun sequence.
ACCESSION   DAZFTN010000510 DAZFTN010000000
VERSION     DAZFTN010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.guided.128"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..161
                     /locus_tag="ACHKV2_005727"
     CDS             <1..161
                     /locus_tag="ACHKV2_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943355.1"
                     /translation="RTLWFELVTKIDMDMHKKNGYASYDLRRKIEMQHEEFQKMTERE
                     QVPLLKSP"
     gene            complement(356..622)
                     /locus_tag="ACHKV2_005728"
     CDS             complement(356..622)
                     /locus_tag="ACHKV2_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943356.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQCLLLTRNISGSVMKK"
     gene            complement(622..1116)
                     /locus_tag="ACHKV2_005729"
     CDS             complement(622..1116)
                     /locus_tag="ACHKV2_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943357.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLKLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKHILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(1172..1774)
                     /locus_tag="ACHKV2_005730"
     CDS             complement(1172..1774)
                     /locus_tag="ACHKV2_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6943358.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            2128..3543
                     /gene="yagA"
                     /locus_tag="ACHKV2_005731"
     CDS             2128..3543
                     /gene="yagA"
                     /locus_tag="ACHKV2_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO6943359.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMEYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFRGLTLSGTKTHVKQR
                     FLPLTPLLMVSDVLWSPVLPA"
     gene            complement(3467..6472)
                     /locus_tag="ACHKV2_005732"
     CDS             complement(3467..6472)
                     /locus_tag="ACHKV2_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFO6943360.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            6636..7193
                     /locus_tag="ACHKV2_005733"
     CDS             6636..7193
                     /locus_tag="ACHKV2_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6943361.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            7376..8236
                     /locus_tag="ACHKV2_005734"
     CDS             7376..8236
                     /locus_tag="ACHKV2_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO6943362.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            8708..10588
                     /locus_tag="ACHKV2_005735"
     CDS             8708..10588
                     /locus_tag="ACHKV2_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO6943363.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLSHSGLDYKRNILNDQNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLSGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFW
                     GI"
     gene            complement(10610..10945)
                     /locus_tag="ACHKV2_005736"
     CDS             complement(10610..10945)
                     /locus_tag="ACHKV2_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HFO6943364.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            11074..11421
                     /locus_tag="ACHKV2_005737"
     CDS             11074..11421
                     /locus_tag="ACHKV2_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO6943365.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCVMWFTYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(11441..12031)
                     /locus_tag="ACHKV2_005738"
     CDS             complement(11441..12031)
                     /locus_tag="ACHKV2_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943366.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(12031..12285)
                     /locus_tag="ACHKV2_005739"
     CDS             complement(12031..12285)
                     /locus_tag="ACHKV2_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6943367.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDNQSARCISELKASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
BASE COUNT         3316 a         2984 c         3026 g         3300 t
ORIGIN      
        1 tgcgaacatt gtggtttgaa ctcgtcacaa aaatagatat ggatatgcat aaaaagaacg
       61 ggtatgcgag ttatgatctt agaaggaaaa tcgaaatgca gcatgaagag tttcagaaaa
      121 tgacagagcg cgagcaagtg cctttattga aatcgccata ataatagaaa cagtacaagt
      181 acattgctgt attgtgtttt ccttgaatat tgaatcgcgg tgcccatgta ctacgattcc
      241 tttcgtgtca tttatcattg cacactttcc ctgactgagc gtaaaacact gactactttc
      301 ccctcaaact cttggacgga gaatgcaaaa ttggtctgtt ccagcataga atatgttact
      361 ttttcatgac cgaaccagaa atattgcgcg tcaataagag acattgtgta aactcagtcc
      421 ttttatctgg caggttaacc cctatcaatc ttaggatgaa accacgccat cctctgtttg
      481 cctggttatt aatttcataa agacgattgt tttcctctcg tagttttgtc cactctaaac
      541 aaagtgttat atcatccagt tctgtgatgt gtttcacttt tccattgaca gttacagtgt
      601 ttattccatt tcctgtgatc atcatcttat gcctttttta tcttcatttg ggaccgtgct
      661 tttcttcgga gtatcactat tcgttaactg ctgtttatca ataaaattcc tgatgttatc
      721 aagtgcttcc tttgttttta gaatgtgctt gatatccatt tcatcccgcc cggtcattct
      781 ttttccggac aaaagacgct caagaagctc ctgtttcgca tcaggagaat ctttggcgag
      841 atccatcagt ttaagcaaat cttcacgatc gagaggaaag aagtatttta caaaatactt
      901 gaaaagcgaa agaaagtagt atgctgtcca tgcaatacag aaaccgattc tgagaaactc
      961 attgtcaact atacagaaat acaagtatac aatcaggaga ataggtaaaa tccataatac
     1021 tatagctaaa aaaatatata cggggatcag gcgtctgtat gcccgttcat gtttattaag
     1081 gatgaataaa atcttctccg cttctgaatc acacataaca ttctcttccc attttttctg
     1141 atacgtaatg tccacctgtt atcaggagtg tttattgccc agaaatattc tcctgctgtg
     1201 cctgcgtcct gagccacttt tgttctaatg tcgccagtct gcgaccggca ctctgtgctt
     1261 cagagtctga gacctgccct ttggggtgac catccaggcc ataacgtggg acgccggcga
     1321 cagctgtccg ctgcagatat acgcggcggg atgtgatgca ggacactgca cagagccatt
     1381 cgtccatggt cagttctgtc tccgggtgtt ctgccagaaa tgccgaaata tcattgctta
     1441 tgccaatttt caggggaata aatccaccac cttcagcacg aagatgagga aacagggcct
     1501 ttattttttc cacgcactca tgagtatact cacgggtcca gtgggaggga cgtggaaaac
     1561 ggtgtctttt ctttttcttc ttcctggctg gcgcttcggg gatatgtgtt tcgacggcag
     1621 atgaatttat tccggcgggg gctgattctg ccgtctgttc agtaaataca ggtgcgataa
     1681 tatctgtttt ttcggataag gacggtggcg aaaaagttcg acgttttttc accacaatga
     1741 cgggagtttt cctttcttcc tgattttttc ccataaatac ctcggctggc tggttttact
     1801 ctttaataat atcacaatcc ataaattagt aaatttccca tcaggtgatt tttcatggct
     1861 aataagtcca ttctctgaat atttatactc gaggtagtta cagttatggc gtgacagtcg
     1921 aattgatatt ctactgaacc ctgatagaaa catggttgat atattctctc actttaatgt
     1981 tgtcatgata taaataaaaa caattcattt gaaaggatat cagaagaaga tatcggggcg
     2041 cacagttcat ctctgccttt gtttcatggg gggttctttg tatattttac ttactgttta
     2101 agcatgtaca taaggagtgc tttcattatg ccaatgatac ttgtaaagaa gaatccccgg
     2161 ggcaaagtga ttcgggagct tagcagtgag gaagagactg cagtaaagac ggtctgtgga
     2221 ctgaaaaggc cagcaaccat ggcacaacat aacctggcca atgatcttct ccgggaaatg
     2281 cgtgagtatg atgcatggtt acagtgtgac tgtattccgg gagattcgcc ggccatgaat
     2341 tttgctgctc ttaaaaacaa tacgggtact ctttatctgt ccagttttaa tcatgagcat
     2401 gcacctgaat gccccatgta tcgtcagtta tccggaaatg aagaggaaac atccttcgga
     2461 gcaagccggc atcccgttag cacccgtata aattacagaa actttttacc acctgatgac
     2521 agtaattctg ttatcagact gcaggcccgc tctgcttacc ataatggtga gcgctcctcc
     2581 gttcgcaaga aacgtccgcg ccttggccgt ttactcctgt cgcttattga agatgcaggg
     2641 ctgaataaac tggattccct ggctaatccc cgtatccgga ctaattatcg tgagtgtctt
     2701 gatgccatac ggcaggttac actacagcag gagtatattc ggggaagagc cttatctgaa
     2761 attatccatt tccggccagg aatgtctgaa aggtctcagg agcggttaat ggaaacgctg
     2821 gagaattcag aacgacactg gcctgccaga agaaagcata tgttctttca gatttttatg
     2881 gcgcagcaca tctgtcgtga tgctgtggaa attcactggg cgaacggtaa tattcaggtc
     2941 atcagacctg tgcgggggat cagtattaat ggtgaagcgc agggcggaat acgtccccct
     3001 tactgggtta ttcttgcttt ttgccggagt gccgatggca gaatcatctg cagtgaaggg
     3061 tatgctcatg ctctttatca actgacatgt ccggtgcctg tggacagcaa actggaacga
     3121 aacacgctca ctgctcttct gaatgtggcc agctggctta aaagaaagcc aggtacgccg
     3181 gaattaagtc tggaaaggcc cctgtttgat acagaagttt atgttaatgg tgaaaagaaa
     3241 tatgtactgc cggatttcat tgtcacagca agggctcctg acggaaagac ggccagagtg
     3301 gtcatcgaaa cgatggaata tgaagacagt gattactgcg cgagaaaatc caggcagcat
     3361 accggcatga agcagattgg tgttctgcat accgatccac cgaaatggct ggataacgat
     3421 catccccctt ttaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gcaacgtttt
     3481 ctgcctctga cgcctctttt aatggtctca gatgtccttt ggtcaccagt tctgccagcg
     3541 tgaaggaata atggccgagc atattgatat gtccgtggca aagcggggag aggcgtgcga
     3601 tatcttcatc attcagtgtt tcaccctgcg cccggagatg atccagggct gcctgcatat
     3661 aaatagtgtt ccataacacg acggcgttag tgaccagccc cagtgtgccc agttgatctt
     3721 cctgaccgtc ggtatatcgt tttcttatct cacctttttg accgtgacag atggctctgg
     3781 caacggcatg gcgactttct ccccgattaa gctgggtcag aatgcgccgg cggtaatctt
     3841 catcatcaat ataattaagc agatacagcg ttttgttgat gcgccccact tcaatgattg
     3901 cctgagtcag tccggaagga cgttcacttt tcagcaatga acggaccagc actgaaacct
     3961 gtactttgcc cagcttcagg gagccagcgg tccggatcat ttcgtcccac tgaaggacta
     4021 tttttcgggg atctgattgc cctctggcaa tatcattcag cacgccatag tcggcatcat
     4081 ggtccattcg ccagaaaacc gaagcaccgg catcagccag gcgtggagaa aactggtatc
     4141 ccagcagcca gaaaaggcca aagacaagtt cgctggcacc tgctgtatcg gtcataattt
     4201 cggttggatt cagcccggtc tcctgttcca gaagaccttc cagcacaaag atagagtccc
     4261 tcagcgtccc cggtataacg atgccatgaa agccggaata ctgatcggac acaaagttgt
     4321 accaggtgat ccctctgtta ttaccaaagt atttgcggtt cggtccggca ttgattgttc
     4381 tgactggcgt aacaaagcgc attccatctg cagatgccac ttctcctcca ccccatatct
     4441 gtgccagtgg cagcgttgcc tgaaaatcaa ccagtctggc attagcgctg gtgatagttt
     4501 cagcccgcag atagttcgct tttgtccagt tcagccggtg tcgggtcagt gcaggaacat
     4561 ttgatctgat cagtggttcc agaccgatat tgcaggcttc agccatcagc acggcgctga
     4621 tgctgacggg cagatcatca actctggcac tggcttcact agcatggaaa aactcatcag
     4681 caaatccggt atgggcgtta atttcgagca gcaactccgt taaatccacc ggagggagta
     4741 gatcactgat cattttgctc agtcgtttca gactgtccgg ctcatcaaga ctggcgaggg
     4801 gagaaattgt caaccggggc ttcgggccag aaacatcgag ttcgacagcc tcattttcgc
     4861 aaagacgtgc agcaacctgt ctgtaacgac tatcaagctg atgacccaga gattttattg
     4921 cttcctgcgg gtctgtcggg tgccccaaag aacgataaac cttaatccgg tttgcctgcc
     4981 agtcagcacc ctgtagtaat cttgcacgag gatctcccca ccggttactg ccggtaacgt
     5041 agacatccct ccgcctcaga ctatcctgca gtttactgag aaagcagagc gtgtatcccc
     5101 tgcgggtgat atgtttttcc ttgttaatca ccagccgttt ccatgaccga ctgataattt
     5161 ccgttggtgc gtcgtcaaaa aactgccgcc gtgagctgaa ctcccggctg aggtagtcac
     5221 aggcattcag agtggtaacc ccggcaggtg cggatgaaaa tttaacggta ttcagcagat
     5281 ggggcaggaa acgacgaacg cgcccgtact gctccaccat ttcttcatga aaattatcgt
     5341 ctgagggccg ggcaatttca cggacaagcg tgatgatttc agccagcttt tgccttggga
     5401 tgtagctgaa cacctcagca cgaatcgatt cgtccggtgt ttcttctttc agcaggtacg
     5461 aacatgcgct ggcgagcgcc aatgcagatt tatccagatc cttcagcgag cggagccgtt
     5521 ttttctgccc aatctttctg gcgtcacgga tgataacggc cagcatggcg tccagaacgt
     5581 ccaatgcatc atccagcgcc agcgtttccc atgcaaggac aaaggcaacc agaaccgcca
     5641 tccttttctg cggtgacatc ctggcaatat tgaacaccga agtcatacca gcataacgtg
     5701 cgagattttt caggcgcaca gccgggagtg tactcaggtt ttcagcatgc aggccaaaat
     5761 cgttcagagt tttccagcgt tcaattgctt cattaaacgc cggaccactg atggtcacag
     5821 ggcccttttt cagtgattcc agtaaagaca ggcggctgca atcagttggc cccagcagca
     5881 tctccagctg tgaacgctgt tcggctgacg gtatcagtgc cagtttgttc cacaggcgca
     5941 acgtcgcctt ttcccttacc tctgaaatca accgggtcag cgtagtggct ccggggagaa
     6001 taatacgatg ttgcataagc caccctgtcg ccagatcgaa aagcaggcca ggacgttcgt
     6061 tgcttatcca gctccgggta tataaaagac gggtaaggcg aaatgtccag ggccaggcaa
     6121 attcacgata ctgatagtgc tgacgtatca gcgctgcatg ctcacggcgg gtattttccc
     6181 tctgaccgta ttctgcaaga acggtgatat cacgaatccc gagctgtctg gcggtaaaat
     6241 gccggacgcc ggaaggaata tgattcatat cggtgaggaa ggtgcccaga aaacggacac
     6301 atccaatttg cagggcaatg cccagacggt tgtgatcacc tctgcttttt ccgataaatt
     6361 ccttgtctgc ttcatcaagg tgaaaatatc gtgccagctg aagctcatcc ggttcaccgg
     6421 tgaatctgcc atagctttca gtctgctcag tggtcagaaa gtcaacgggc atatcggcct
     6481 ccctgcctga cgggcattta gtaacatttt tccaaccgta cgaaatgtta taaattatcg
     6541 gacatcgtaa aactgttaca ttaatatgtc tattaaatcg taaatttgta ataatagaca
     6601 tgagttgtcc gatattcgat ttaaggtaca tttttatgcg actttttggt tacgctcggg
     6661 tctcaaccag tcagcagtct cttgatcttc aggtcagagc actcaaagac gcaggtgtga
     6721 aagcaaaccg tatatttacc gataaggcat ccggcagttc aacagaccgg gaagggctgg
     6781 atttgctgag gatgaaggtg gaggaaggtg atgtcattct ggttaagaag ctcgaccgtc
     6841 ttggccgcga cactgccgat atgatccaac tgataaagga atttgacgct cagggcgtgg
     6901 cagtccggtt cattgatgac gggatcagta ccgacggtga tatggggcaa atggtggtca
     6961 ccatcctgtc ggctgtggca caggctgaac gccggaggat cctagaacgc acgaatgagg
     7021 gccgacagga agcaaagctg aaaggaatca aatttggccg caggcgtacc gtggacagga
     7081 acgtcgtgct gacgcttcat cagaagggca ctggtgcaac ggaaattgct catcagctca
     7141 gtattgcccg ctccacggtt tataaaattc ttgaagacga aagggcctcg tgatacgcct
     7201 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7261 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7321 gctcatgaga caataaccct ggtaaatgct tcaataatat tgaaaaagga agagtatgag
     7381 tattcaacat tttcgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7441 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7501 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7561 acgttttcca atgatgagca cttttaaagt tctgctatgt ggtgcggtat tatcccgtgt
     7621 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7681 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7741 tgctgccata accatgagtg ataacactgc tgccaactta cttctgacaa cgatcggagg
     7801 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7861 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     7921 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7981 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8041 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8101 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8161 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8221 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8281 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8341 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttttaag aaacatatgt
     8401 acggtgtttt tatgcatctc aggtactgag atattttgtg gctcagttct gtaacttttc
     8461 ccgtaacatt gtctgttgtt acgggaaagt ccggtttttg tattgcacca gagaataccc
     8521 agactgtgat gctgccacag cgtcagcagg ctttctgaac ggtgtaacat cacttcatgt
     8581 taatgataat cactatcatc aaatcttgac atgccatttt ctccttaata aattaatact
     8641 gtatatgtat ccatatgcgt aagcagttaa ttcatttgtt ttcctcagag gatgaaggag
     8701 ataccgaatg tctgaccctg tacgtattac aaatcccggt gcagaatcgc tggggtatga
     8761 ttcagatggc catgaaatta tggccgttga tatttatgta aaccctccac gtgtcgatgt
     8821 ctttcatggt accccgcctg catggagttc cttcgggaac aaaaccatct ggggcggaaa
     8881 cgagtgggtt gatgattccc caacccgaag tgatatcgaa aaaagggaca aggaaatcac
     8941 agcgtacaaa aacacgctca gcgcgcagca gaaagagaat gagaataagc gtactgaagc
     9001 cggaaaacgc ctctctgcgg cgattgctgc aagggaaaaa gatgaaaaca cactgaaaac
     9061 actccgtgcc ggaaacgcag atgccgctga tattacacga caggagttca gactcctgca
     9121 ggcagagctg agagaatacg gattccgtac tgaaatcgcc ggatatgacg ccctccggct
     9181 gcatacagag agccggatgc tgtttgctga tgctgattct cttcgtatat ctccccggga
     9241 ggccaggtcg ttaatcgaac aggctgaaaa acggcagaag gatgcgcaga acgcagacaa
     9301 gaaggccgct gatatgcttg ctgaatacga gcgcagaaaa ggtattctgg acacccggtt
     9361 gtcagagctg gaaaaaaatg gcggggcagc ccttgccgtt cttgatgcac aacaggcccg
     9421 tctgctcggg cagcagacac ggaatgacag ggccatttca gaggcccgga ataaactcag
     9481 ttcagtgacg gaatcgctta acacggcccg taatgcatta accagagctg aacaacagct
     9541 gacgcaacag aaaaacacgc ctgacggcaa aacgatagtt tcccctgaaa aattcccggg
     9601 gcgttcatca acaaatcatt ctattgttgt gagcggtgat ccgagatttg ccggtacgat
     9661 aaaaatcaca accagcgcag tcatcgataa ccgtgcaaac ctgaattatc ttctgagcca
     9721 ttccggtctg gactataaac gcaatattct gaatgaccag aatccggtgg tgacagagga
     9781 tgtggaaggt gacaagaaaa tttataatgc tgaagttgct gaatgggata agttacggca
     9841 aagattgctt gatgccagaa ataaaatcac ctctgctgaa tctgcggtaa attcggcgag
     9901 aaataacctc agtgccagaa caaatgagca aaagcatgca aatgacgctc ttaatgccct
     9961 gttgaaggaa aaagagaata tccgtaacca gctttccggc atcaatcaga agatagcgga
    10021 agagaaaaga aaacaggatg aactgaaggc aacgaaagac gcaattaatt tcacaacaga
    10081 gttcctgaaa tcagtttcag aaaaatatgg tgcaaaagct gagcagttag ccagagagat
    10141 ggccgggcag gctaaaggga agaaaatacg taatgttgaa gaggcattaa aaacgtatga
    10201 aaagtaccgg gctgacatta acaaaaaaat taatgcaaaa gatcgtgcag cgattgccgc
    10261 agcccttgag tctgtgaagc tgtctgatat atcgtctaat ctgaacagat tcagtcgggg
    10321 actgggatat gcaggaaaat ttacaagtct tgctgactgg atcactgagt ttggtaaggc
    10381 tgtccggaca gagaactggc gtcctctttt tgttaaaaca gaaaccatca tagcaggcaa
    10441 tgccgcaacg gctcttgtgg cactggtctt cagtattctt accggaagcg ctttaggcat
    10501 tatcgggtat ggtttactga tggctgtcac cggtgcgctg attgatgaat cgcttgtgga
    10561 aaaagcgaat aagttctggg gtatttaaat aaaatataag acaggctgtc tatcttacag
    10621 acagcctttt tataataaca gaaagaatat atatcatact taacggaaga gataatatga
    10681 aaacagtacc cgtataaacc gcaagcaacc ccatttttcc aggtgtatca gcaaaaaaac
    10741 cgctgttcca gaaatcaggc cgggtaaatt tcagagctgt atcttcaata taccattttg
    10801 caaccggata caaaaccatt ccgcagagag aaatacacca gaacagtaat ctgtatttaa
    10861 actcataatc ccatgacata tacagcatat atccccccgt cacccatccc caccacatat
    10921 tattaaaata atattttctg ttcatctgcc agttttcctt tcttttgcac ttttgtcagt
    10981 gtactgatgc atgacaacat aaccacagca tgataataat aatcaataac aataagctgt
    11041 gtcacgttta catcatggaa cggaggtgct cctgtgacct ttgagcagaa aaaagcccgg
    11101 gccattgccc tgatggacag caaaaagatg tggcggagca attatgcccc tcctcttctg
    11161 cgtattctat ggcggctggg tatccggctt ccgccattac cctttatgcc gttctggcag
    11221 gtcacggtgc tgacgggggg cttatggggc atttcctggg gatgtgtaat gtggtttacc
    11281 tactgggggc cgtcaggaat ggtggcaggt gaggccatta ttataagtat cacgggtggt
    11341 tttttgttcg gactgtgcat ggcctctttc cactggtggc gcaggaaagt taaccggctg
    11401 ccaccatggg atgatgtgta aaccgtgcac aggcgtgttc ttacggagcg gcactttcat
    11461 ttatctgtac gcgaacctct attcctgcgt catgacaggc ctgcagccac tgcgccactt
    11521 ccagcggatc gccctcccgg cgtaccaccc tgccttcttt attccataac tgcagacagg
    11581 tgctgccgtc gagacgcacc acaaaatccc cgcggcaggc ctgatacgtt atgccgttca
    11641 gctccaccgc cagatcagtg gcagaagtgc cgtctgcggg cagacctgct gccgggacca
    11701 gcggcagtac gacgacttct gccgcacgct gttctgcccg gaggcggtcc tgttctgcca
    11761 gcagcagagg ctgtgccacg gcccggccgg cgtccgtcag ctccactgcc agctgcaggt
    11821 tcggggcgcg cagggtacgc agccagccgg catcctccag acgacggcag gaggcgcgca
    11881 ggtttggccc gtagaccggg gcttccccgc ttcgctccag cacccgttcc aggtcccgcg
    11941 tcagcacggg gcccggccgt ttttcatcaa gcgcagccag cacaatcagc actctccgct
    12001 gcagtggcga cggtcggcgg ctctgagtca ttaactgtca ccctccgacc agatgagttt
    12061 tttgaggcgc tgataatcgg tatcgatgcg ttcccgggcc tgtgcgtgat tgttctgtgg
    12121 cgcaccatga atggccagtt cataccacaa ccacagcaga gctgaggcct tcagttcact
    12181 gatacacctg gcgctctggt tgtccagccc acattcacgg atattttttt ccgctattcg
    12241 ggttagttgc tcatagctga gtgaaaacac cctttcttca tccatcctgc acgctccatc
    12301 tgataatgtt ctgacaggta aaaataatat cctgtatgat ttgtgtgctc ctgatttctg
    12361 ataatatcag atatggtttt atcatcaatg atgaaaatca tatctgatat gattttgtgt
    12421 gtattgctca attaccatat cacttgcccg tttcccgccg cgcagatccg gtttccggcg
    12481 gttcaggatc ttcctctccc cccacataac gccacctcct gtcagcacaa catgtggtat
    12541 ctcattcagc tgcttatgac actatatgtt gtgtcatctc cctgacctgt gatgcgccgc
    12601 gcaggggcgg aaaacagcga tatgat
//
LOCUS       DAZFTN010000511         6077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.guided.206, whole genome shotgun sequence.
ACCESSION   DAZFTN010000511 DAZFTN010000000
VERSION     DAZFTN010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.guided.206"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..302
                     /locus_tag="ACHKV2_005740"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHKV2_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHKV2_005741"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHKV2_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHKV2_005742"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHKV2_005742"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6943368.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHKV2_005743"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHKV2_005743"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6943369.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHKV2_005744"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHKV2_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6943370.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHKV2_005745"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHKV2_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6943371.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..5944)
                     /gene="phoE"
                     /locus_tag="ACHKV2_005746"
     CDS             complement(4889..5944)
                     /gene="phoE"
                     /locus_tag="ACHKV2_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6943372.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1484 a         1496 c         1538 g         1559 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     5461 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
     5521 tgtcggtctg cgccgaggag tcgccaccaa attccgggaa catatcggtc caggcttcca
     5581 cgtcatacaa cgcgcccagg ttacggccat agtcgaaaga acccaaatct ttatacttca
     5641 atccggcaaa agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg
     5701 caaactccgc ttcccaacga ccataaccag tcagttgatc gttaatttgt gtttcgcctt
     5761 taaaaccaaa acggatataa ctctggtcgc catctttact gtcgttatca ctcatataat
     5821 gcatggcttt aactttgcca tagacatcca gtttattacc gtctttgtta tatatttctg
     5881 cggcctgtac ggatgcagat gccacaatgc ccatcaccac taatgccaga gtgctctttt
     5941 tcattttcat tcctgatttt aattaacgcg cgaatattct gcgggagagc cccgttgaaa
     6001 acaggaaagt ttttaacctg agattgttaa agatatatta cagattaatg atattcttaa
     6061 aatgtggtaa tttatta
//
LOCUS       DAZFTN010000512         1927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2309H31198
            SAMN43283879-rid23073483.guided.308, whole genome shotgun sequence.
ACCESSION   DAZFTN010000512 DAZFTN010000000
VERSION     DAZFTN010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283879
            Sequence Read Archive: SRR30325391
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351653.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351653.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:45:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,749
            CDSs (total)                      :: 5,653
            Genes (coding)                    :: 5,397
            CDSs (with protein)               :: 5,397
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 100 of 256
            Pseudo Genes (incomplete)         :: 143 of 256
            Pseudo Genes (internal stop)      :: 66 of 256
            Pseudo Genes (multiple problems)  :: 42 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283879-rid23073483.guided.308"
                     /serotype="O157:H 7"
                     /isolate="2309H31198"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-08"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..150
                     /locus_tag="ACHKV2_005747"
     tRNA            74..150
                     /locus_tag="ACHKV2_005747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:108..110,aa:Arg,seq:tct)
     gene            241..1200
                     /locus_tag="ACHKV2_005748"
     CDS             241..1200
                     /locus_tag="ACHKV2_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO6943373.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1212..1481
                     /locus_tag="ACHKV2_005749"
     CDS             1212..1481
                     /locus_tag="ACHKV2_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO6943374.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          498 a          410 c          482 g          537 t
ORIGIN      
        1 ggacagagcg ccagccttcg aagctggctg cgcggggttc gagtcctcga tggcggtcca
       61 ttatctgcat tatgcgttgt tagctcagcc ggacagagca attgccttct gagcaatcgg
      121 tcactggttc gaatccagta caacgcgcca tatttattta ccaggctcgc ttttgcgggc
      181 cttttttata tctgcgccgg gtctggtgct gattacttca gccaaaagga acacctgtat
      241 atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc ttcggtatcc
      301 tattcccggg agtttacgat agacttttcg acccaacaaa gttatgtctc ttcgttaaat
      361 agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg
      421 tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat
      481 gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg
      541 gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata
      601 tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa
      661 cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca
      721 tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt
      781 ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt
      841 cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc
      901 actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc
      961 agagtgggga gaatatcctt taataatata tcagcgatac tggggactgt ggccgttata
     1021 ctgaattgcc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga gagtcaacca
     1081 gaatgtcaga taactggcga caggcccgtt ataaaaataa acaatacatt atgggaaagt
     1141 aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa
     1201 aggagttaag catgaagaag atgtttatgg cggttttatt tgcattagct tctgttaatg
     1261 caatggcggc ggattgtgct aaaggtaaaa ttgagttttc caagtataat gaggatgaca
     1321 catttacagt gaaggttgac gggaaagaat actggaccag tcgctggaat ctgcaaccgt
     1381 tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat
     1441 caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc tgattcgtgg
     1501 tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt gcctgtcaga
     1561 ctgagcattt gttaaaaaaa tttcgcatgg tgaatccccc tgtgtggagg ggcgactggt
     1621 gaaaaatcct tgcttgtgat tcattatcga cacgggttcg gtggtaccag gccgaactca
     1681 ccgggaggca cccggcacca tgcagtatac agagattagg catataccaa ggcctctcat
     1741 agcaggggcc tttttacatg taaaaaagcc cgagtgggtt cgggcaattg catgagatac
     1801 tcgttttaat aatcgaaagc attttaacca ggattcataa ggctgcgcaa ctgcgcggcc
     1861 tttttcgtat ttcgggctgt agtcttcctt ctgccattgt cctgtaactt ccggacttca
     1921 gcccgct
//
